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    <name>D3DXL3_CUPMC</name>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
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    <gene>
        <name evidence="2" type="ordered locus">Rmet_6415</name>
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    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
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        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
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            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_6415"/>
    <dbReference type="EnsemblBacteria" id="ADC45033">
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        <property type="gene ID" value="Rmet_6415"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6415"/>
    <dbReference type="HOGENOM" id="CLU_2882715_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="1"/>
            <end position="41"/>
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    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADC45033.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="63" mass="6587" checksum="F97EBB47264D9CCB" modified="2010-03-23" version="1">MRARQSAKAKVGNVPKSLVAPPAKTGPVDRPVSPPPSSFGGFEPKSNRLGFFFVFCKGGPAKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-03-23" modified="2020-10-07" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>D3DXU3</accession>
    <name>D3DXU3_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_6505</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
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            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ADC45113.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_6505"/>
    <dbReference type="EnsemblBacteria" id="ADC45113">
        <property type="protein sequence ID" value="ADC45113"/>
        <property type="gene ID" value="Rmet_6505"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6505"/>
    <dbReference type="HOGENOM" id="CLU_2919460_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADC45113.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="61" mass="7005" checksum="E5CBC4F3F7499C5D" modified="2010-03-23" version="1">MRANPSQCTFFGKSGVWQAERWLRLARFLLTFDVPFGAVQADDRTNFGLPTFPVTARISRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-03-23" modified="2020-10-07" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>D3DY53</accession>
    <name>D3DY53_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_6623</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ADC45223.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_6623"/>
    <dbReference type="EnsemblBacteria" id="ADC45223">
        <property type="protein sequence ID" value="ADC45223"/>
        <property type="gene ID" value="Rmet_6623"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6623"/>
    <dbReference type="HOGENOM" id="CLU_2919465_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADC45223.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="61" mass="6780" checksum="93F50A7260C03851" modified="2010-03-23" version="1">MVANATRLHHLEEENDEPIQQDLPPETGVPHKQPYEQSYKLPYTPTVSPGKLAPPCTARSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-03-23" modified="2020-10-07" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>D3DYE4</accession>
    <name>D3DYE4_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_6735</name>
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    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ADC45314.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADC45314">
        <property type="protein sequence ID" value="ADC45314"/>
        <property type="gene ID" value="Rmet_6735"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6735"/>
    <dbReference type="HOGENOM" id="CLU_3011053_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADC45314.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="56" mass="6319" checksum="DAB9B2A906D48EBB" modified="2010-03-23" version="1">MTVGISVLADGRRHPSLRIGGLRLSLSYLKKDTLTKASNFSREEMFRVIPFLGARR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-03-23" modified="2020-10-07" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>D3DYH0</accession>
    <name>D3DYH0_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_6761</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ADC45340.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADC45340">
        <property type="protein sequence ID" value="ADC45340"/>
        <property type="gene ID" value="Rmet_6761"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6761"/>
    <dbReference type="HOGENOM" id="CLU_2510303_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADC45340.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="85" mass="9416" checksum="7AABC593DC0D1AD9" modified="2010-03-23" version="1">MLGPVGAIRSVCRSRSTVHGVRRKRNRHYLSRTSMRFANWKEGQVTVEVPVFPGCQSGGVLDLPHRGSVRHCINLPLVAMAAHGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="50" xmlns="http://uniprot.org/uniprot">
    <accession>Q1L9Q4</accession>
    <name>Q1L9Q4_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Rhs-related transmembrane protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">rhsA</name>
        <name evidence="2" type="ordered locus">Rmet_6263</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2 3">
        <name>pMOL28</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid pMOL28</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000355">
        <property type="protein sequence ID" value="ABF13122.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF13122">
        <property type="protein sequence ID" value="ABF13122"/>
        <property type="gene ID" value="Rmet_6263"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6263"/>
    <dbReference type="HOGENOM" id="CLU_312350_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid pMOL28"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045351">
        <property type="entry name" value="DUF6531"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031325">
        <property type="entry name" value="RHS_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006530">
        <property type="entry name" value="YD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20148">
        <property type="entry name" value="DUF6531"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05593">
        <property type="entry name" value="RHS_repeat"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01643">
        <property type="entry name" value="YD_repeat_2x"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <feature type="domain" description="DUF6531" evidence="1">
        <location>
            <begin position="187"/>
            <end position="266"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF20148"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF13122.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="939" mass="101050" checksum="795AD406CC8660D4" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="61" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LAK0</accession>
    <name>Q1LAK0_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Bactoprenol-linked glucose translocase (Flippase) GtrA</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">gtrA1</name>
        <name evidence="8" type="ordered locus">Rmet_5967</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="8 9">
        <name>pMOL30</name>
    </geneLocation>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="9">Plasmid pMOL30</plasmid>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the GtrA family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000354">
        <property type="protein sequence ID" value="ABF12826.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008650722.1">
        <property type="nucleotide sequence ID" value="NC_007971.2"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF12826">
        <property type="protein sequence ID" value="ABF12826"/>
        <property type="gene ID" value="Rmet_5967"/>
    </dbReference>
    <dbReference type="GeneID" id="60820433"/>
    <dbReference type="KEGG" id="rme:Rmet_5967"/>
    <dbReference type="HOGENOM" id="CLU_083873_6_2_4"/>
    <dbReference type="OMA" id="LASWWRF"/>
    <dbReference type="OrthoDB" id="1813832at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid pMOL30"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000271">
        <property type="term" value="P:polysaccharide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007267">
        <property type="entry name" value="GtrA"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04138">
        <property type="entry name" value="GtrA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0614">Plasmid</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="20"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="69"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="95"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="domain" description="GtrA" evidence="7">
        <location>
            <begin position="9"/>
            <end position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009399"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF04138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ABF12826.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="127" mass="13752" checksum="6092A75074C0DCB5" modified="2006-05-30" version="1">MNIPSRFVRFLGAGATATATHYATLITLVGFGVAALPASAAGSVAGLLTHYCISSRWVFTPVRPQRATFGRFILVSALAFFLNSLLMWAGLALGLHYLVAQILSTIFVMLVNYVLHANWTFMNGAMR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="99" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LAQ4</accession>
    <name>Q1LAQ4_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Methylated-DNA--protein-cysteine methyltransferase</fullName>
            <ecNumber evidence="7">2.1.1.63</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">6-O-methylguanine-DNA methyltransferase</fullName>
            <shortName evidence="7">MGMT</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="7">O-6-methylguanine-DNA-alkyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">ogt3</name>
        <name evidence="9" type="ordered locus">Rmet_5913</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="10">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="10">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) and O4-methylthymine (O4-MeT) in DNA. Repairs the methylated nucleobase in DNA by stoichiometrically transferring the methyl group to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 7">
            <text>a 4-O-methyl-thymidine in DNA + L-cysteinyl-[protein] = a thymidine in DNA + S-methyl-L-cysteinyl-[protein]</text>
            <dbReference type="Rhea" id="RHEA:53428"/>
            <dbReference type="Rhea" id="RHEA-COMP:10131"/>
            <dbReference type="Rhea" id="RHEA-COMP:10132"/>
            <dbReference type="Rhea" id="RHEA-COMP:13555"/>
            <dbReference type="Rhea" id="RHEA-COMP:13556"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:82612"/>
            <dbReference type="ChEBI" id="CHEBI:137386"/>
            <dbReference type="ChEBI" id="CHEBI:137387"/>
            <dbReference type="EC" id="2.1.1.63"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2 7">
            <text>a 6-O-methyl-2'-deoxyguanosine in DNA + L-cysteinyl-[protein] = a 2'-deoxyguanosine in DNA + S-methyl-L-cysteinyl-[protein]</text>
            <dbReference type="Rhea" id="RHEA:24000"/>
            <dbReference type="Rhea" id="RHEA-COMP:10131"/>
            <dbReference type="Rhea" id="RHEA-COMP:10132"/>
            <dbReference type="Rhea" id="RHEA-COMP:11367"/>
            <dbReference type="Rhea" id="RHEA-COMP:11368"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:82612"/>
            <dbReference type="ChEBI" id="CHEBI:85445"/>
            <dbReference type="ChEBI" id="CHEBI:85448"/>
            <dbReference type="EC" id="2.1.1.63"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="miscellaneous">
        <text evidence="7">This enzyme catalyzes only one turnover and therefore is not strictly catalytic. According to one definition, an enzyme is a biocatalyst that acts repeatedly and over many reaction cycles.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the MGMT family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.63" evidence="7"/>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12772.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518122.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5913"/>
    <dbReference type="EnsemblBacteria" id="ABF12772">
        <property type="protein sequence ID" value="ABF12772"/>
        <property type="gene ID" value="Rmet_5913"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5913"/>
    <dbReference type="eggNOG" id="COG0350">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_52_2_4"/>
    <dbReference type="OMA" id="LVVRPCH"/>
    <dbReference type="OrthoDB" id="1327994at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003908">
        <property type="term" value="F:methylated-DNA-[protein]-cysteine S-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006307">
        <property type="term" value="P:DNA dealkylation involved in DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06445">
        <property type="entry name" value="ATase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00772">
        <property type="entry name" value="OGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001497">
        <property type="entry name" value="MethylDNA_cys_MeTrfase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014048">
        <property type="entry name" value="MethylDNA_cys_MeTrfase_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036217">
        <property type="entry name" value="MethylDNA_cys_MeTrfase_DNAb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023546">
        <property type="entry name" value="MGMT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036631">
        <property type="entry name" value="MGMT_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01035">
        <property type="entry name" value="DNA_binding_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46767">
        <property type="entry name" value="SSF46767"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53155">
        <property type="entry name" value="SSF53155"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00589">
        <property type="entry name" value="ogt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00374">
        <property type="entry name" value="MGMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0227">DNA damage</keyword>
    <keyword evidence="6 7" id="KW-0234">DNA repair</keyword>
    <keyword evidence="4 7 9" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="9" id="KW-0614">Plasmid</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7 9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="DNA_binding_1" evidence="8">
        <location>
            <begin position="82"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile; methyl group acceptor" evidence="7">
        <location>
            <position position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001596"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00772"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ABF12772.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="182" mass="18877" checksum="398758E9F3A2048B" modified="2006-05-30" version="1">MSSHHIIDSPLGDMLLRADGGALTGVFFAGQKYYPGLARPTPVSAMPAADARVLEQAAMELGAYFDGALHGFSVPFRLVGSAFQQRIWQALCDIEFGETSTYGQLGAGLGLPPSHSRAVGGAVGRNPLSIIVPCHRVLGASGDLTGYAGGVDRKRALLTLEGVLYPGQLQATALGQAALALD</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LAR1</accession>
    <name>Q1LAR1_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Alpha-ketoglutarate transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">kgtP</name>
        <name evidence="7" type="ordered locus">Rmet_5906</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="8">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="8">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12765.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518113.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5906"/>
    <dbReference type="EnsemblBacteria" id="ABF12765">
        <property type="protein sequence ID" value="ABF12765"/>
        <property type="gene ID" value="Rmet_5906"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5906"/>
    <dbReference type="eggNOG" id="COG0477">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001265_39_0_4"/>
    <dbReference type="OMA" id="THTNDPT"/>
    <dbReference type="OrthoDB" id="955823at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004736">
        <property type="entry name" value="MHS_symport"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="SSF103473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00883">
        <property type="entry name" value="2A0106"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00217">
        <property type="entry name" value="SUGAR_TRANSPORT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0614">Plasmid</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="23"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="64"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="98"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="127"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="166"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="197"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="246"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="285"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="315"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="342"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="375"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="408"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="domain" description="MFS" evidence="6">
        <location>
            <begin position="25"/>
            <end position="431"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABF12765.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="437" mass="47270" checksum="02CC0937AB77A01D" modified="2006-05-30" version="1">MTDTASSVTTGTAPSPEEVKKRIFAIVAASSGNLVEWFDFYVYAFSAIYFASSFFPKADPTAQLLNTAGVFAAGFLMRPIGGWLFGRIADRTGRKHSMVISVTMMCFGSLMIACLPTYAAIGNLAPALLLLARLLQGLSVGGEYGTTATYMSEVALKGRRGFFSSFQYVTLIGGQLLAVLVIVILEQLLTEAELKAWGWRIPFVVGAVTAVVALLLRRTLHETTTAQSRNSKGAGTVGELFRHHKAAFFTVLGYTAGGSLIFYTFTTYMQKYLVNTAGMPIKTASYVMTGCLFLYMCMQPLFGMLSDRIGRRNNMLMFGALGAVATVPILTALKTVSSPEMALLLICVALAIVSFYTSISGIVKAEMFPPEVRALGVGLAYAVANAIFGGSAEYVALGLKSIGHESAFYWYVTAMMVVAFLVSLRLPRQAKYLHHEH</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="114" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LAT0</accession>
    <name>Q1LAT0_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">2-isopropylmalate synthase</fullName>
            <ecNumber evidence="3">2.3.3.13</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">Alpha-IPM synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="3">Alpha-isopropylmalate synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5" type="primary">leuA1</name>
        <name evidence="3" type="synonym">leuA</name>
        <name evidence="5" type="ordered locus">Rmet_5887</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="6">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="6">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>3-methyl-2-oxobutanoate + acetyl-CoA + H2O = (2S)-2-isopropylmalate + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:21524"/>
            <dbReference type="ChEBI" id="CHEBI:1178"/>
            <dbReference type="ChEBI" id="CHEBI:11851"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="EC" id="2.3.3.13"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Amino-acid biosynthesis; L-leucine biosynthesis; L-leucine from 3-methyl-2-oxobutanoate: step 1/4.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homotetramer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 2 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.3.3.13" evidence="3"/>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12746.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518093.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5887"/>
    <dbReference type="EnsemblBacteria" id="ABF12746">
        <property type="protein sequence ID" value="ABF12746"/>
        <property type="gene ID" value="Rmet_5887"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5887"/>
    <dbReference type="eggNOG" id="COG0119">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004588_3_0_4"/>
    <dbReference type="OMA" id="WPDKVID"/>
    <dbReference type="OrthoDB" id="840579at2"/>
    <dbReference type="UniPathway" id="UPA00048">
        <property type="reaction ID" value="UER00070"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003852">
        <property type="term" value="F:2-isopropylmalate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009098">
        <property type="term" value="P:leucine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd07942">
        <property type="entry name" value="DRE_TIM_LeuA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00572">
        <property type="entry name" value="LeuA_type2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013709">
        <property type="entry name" value="2-isopropylmalate_synth_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002034">
        <property type="entry name" value="AIPM/Hcit_synth_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005668">
        <property type="entry name" value="IPM_Synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036230">
        <property type="entry name" value="LeuA_allosteric_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039371">
        <property type="entry name" value="LeuA_N_DRE-TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000891">
        <property type="entry name" value="PYR_CT"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00682">
        <property type="entry name" value="HMGL-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08502">
        <property type="entry name" value="LeuA_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00917">
        <property type="entry name" value="LeuA_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110921">
        <property type="entry name" value="SSF110921"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00970">
        <property type="entry name" value="leuA_yeast"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00815">
        <property type="entry name" value="AIPM_HOMOCIT_SYNTH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00816">
        <property type="entry name" value="AIPM_HOMOCIT_SYNTH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50991">
        <property type="entry name" value="PYR_CT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="3" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="2 3" id="KW-0100">Branched-chain amino acid biosynthesis</keyword>
    <keyword evidence="3" id="KW-0432">Leucine biosynthesis</keyword>
    <keyword evidence="5" id="KW-0614">Plasmid</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 3 5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Pyruvate carboxyltransferase" evidence="4">
        <location>
            <begin position="33"/>
            <end position="307"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00572"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABF12746.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="567" mass="63097" checksum="AB4DC5349AD774AD" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LB51</accession>
    <name>Q1LB51_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_5766</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12625.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF12625">
        <property type="protein sequence ID" value="ABF12625"/>
        <property type="gene ID" value="Rmet_5766"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5766"/>
    <dbReference type="HOGENOM" id="CLU_2510305_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="62"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="69"/>
            <end position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF12625.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="85" mass="9387" checksum="03D8E6AFFF3D456F" modified="2006-05-30" version="1">MASTRADRTRFLSSTATTISPRDGLRISEKRIQAGLERPNAVANMPTPREARNQTEIASMVADPMSQSDRRANVASPQYGNIRNQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-10-07" version="51" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LB78</accession>
    <name>Q1LB78_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_5739</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12598.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5739"/>
    <dbReference type="EnsemblBacteria" id="ABF12598">
        <property type="protein sequence ID" value="ABF12598"/>
        <property type="gene ID" value="Rmet_5739"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5739"/>
    <dbReference type="HOGENOM" id="CLU_2106980_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF12598.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="115" mass="13223" checksum="ABD6AED73E61E27A" modified="2006-05-30" version="1">MHSQYAFLQCKAPFTECVPSYELPQEHRMTARTHQRSLYEMVFLYLNRRPGQRFAACQLARALNVRTIMILDAIDILSEQGRVVAEPLTPEQLGYFVAPAQTNQCSTCSNRIDKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="63" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LBN3</accession>
    <name>Q1LBN3_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_5584</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12443.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519992.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5584"/>
    <dbReference type="EnsemblBacteria" id="ABF12443">
        <property type="protein sequence ID" value="ABF12443"/>
        <property type="gene ID" value="Rmet_5584"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5584"/>
    <dbReference type="eggNOG" id="ENOG5032YBS">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_090266_1_0_4"/>
    <dbReference type="OMA" id="PAHFSAY"/>
    <dbReference type="OrthoDB" id="1899049at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004192987" evidence="1">
        <location>
            <begin position="21"/>
            <end position="170"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF12443.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="170" mass="19471" checksum="94D5D86CB14029A9" modified="2006-05-30" version="1">MQLRLPVWMGIAALVLSACASLQPVETGQKMIGSPQSAVSDTFGKPTETHQLADGTLRWIYSKQPYGYDVYAADFDGSGKLIAFRQMLTEAEIYKAQPNTWTKRDVMERWGMPREPIQYYPLMKREAWSYRMYLPGYVPAHFSAYFDDNGVLDRTMIIMDTYGADRSRSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LC78</accession>
    <name>Q1LC78_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Oxidoreductase FAD/NAD(P)-binding</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Rmet_5389</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="8">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="8">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12248.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519794.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5389"/>
    <dbReference type="EnsemblBacteria" id="ABF12248">
        <property type="protein sequence ID" value="ABF12248"/>
        <property type="gene ID" value="Rmet_5389"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5389"/>
    <dbReference type="eggNOG" id="COG0369">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG3182">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001570_17_4_4"/>
    <dbReference type="OMA" id="SEGGTTW"/>
    <dbReference type="OrthoDB" id="106626at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008333">
        <property type="entry name" value="Cbr1-like_FAD-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017927">
        <property type="entry name" value="FAD-bd_FR_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008254">
        <property type="entry name" value="Flavodoxin/NO_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001709">
        <property type="entry name" value="Flavoprot_Pyr_Nucl_cyt_Rdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029039">
        <property type="entry name" value="Flavoprotein-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039261">
        <property type="entry name" value="FNR_nucleotide-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001433">
        <property type="entry name" value="OxRdtase_FAD/NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005625">
        <property type="entry name" value="PepSY-ass_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017938">
        <property type="entry name" value="Riboflavin_synthase-like_b-brl"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00970">
        <property type="entry name" value="FAD_binding_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00258">
        <property type="entry name" value="Flavodoxin_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00175">
        <property type="entry name" value="NAD_binding_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03929">
        <property type="entry name" value="PepSY_TM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00371">
        <property type="entry name" value="FPNCR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52218">
        <property type="entry name" value="SSF52218"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52343">
        <property type="entry name" value="SSF52343"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63380">
        <property type="entry name" value="SSF63380"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51384">
        <property type="entry name" value="FAD_FR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50902">
        <property type="entry name" value="FLAVODOXIN_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="3" id="KW-0288">FMN</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0614">Plasmid</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="7"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="123"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="172"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="289"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="domain" description="Flavodoxin-like" evidence="5">
        <location>
            <begin position="334"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="domain" description="FAD-binding FR-type" evidence="6">
        <location>
            <begin position="485"/>
            <end position="595"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABF12248.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="732" mass="78312" checksum="25453489DD5DE675" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LC79</accession>
    <name>Q1LC79_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 8 10">FAD:protein FMN transferase</fullName>
            <ecNumber evidence="2 8 10">2.7.1.180</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Flavin transferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">apbE</name>
        <name evidence="11" type="ordered locus">Rmet_5388</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="12">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="12">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Flavin transferase that catalyzes the transfer of the FMN moiety of FAD and its covalent binding to the hydroxyl group of a threonine residue in a target flavoprotein.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 8 10">
            <text>FAD + L-threonyl-[protein] = AMP + FMN-L-threonyl-[protein] + H(+)</text>
            <dbReference type="Rhea" id="RHEA:36847"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11061"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
            <dbReference type="ChEBI" id="CHEBI:74257"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="2.7.1.180"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <cofactor evidence="9">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="9">Magnesium. Can also use manganese.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Cell inner membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8 10">Belongs to the ApbE family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.180" evidence="2 8 10"/>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12247.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519793.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5388"/>
    <dbReference type="EnsemblBacteria" id="ABF12247">
        <property type="protein sequence ID" value="ABF12247"/>
        <property type="gene ID" value="Rmet_5388"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5388"/>
    <dbReference type="eggNOG" id="COG1477">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_044403_0_2_4"/>
    <dbReference type="OMA" id="LINIWGF"/>
    <dbReference type="OrthoDB" id="2021063at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017013">
        <property type="term" value="P:protein flavinylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.520.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024932">
        <property type="entry name" value="ApbE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003374">
        <property type="entry name" value="ApbE-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30040">
        <property type="entry name" value="PTHR30040"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02424">
        <property type="entry name" value="ApbE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006268">
        <property type="entry name" value="ApbE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143631">
        <property type="entry name" value="SSF143631"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="10" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8 10" id="KW-0274">FAD</keyword>
    <keyword evidence="4 8 10" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="10 11" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="7 8 9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 8 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0614">Plasmid</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8 10" id="KW-0808">Transferase</keyword>
    <feature type="binding site" evidence="9">
        <location>
            <position position="165"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="279"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="283"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001219"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011955"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00016337"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRNR" id="PIRNR006268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR006268-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU363002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ABF12247.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="329" mass="34425" checksum="8D636AC4109EDE3D" modified="2006-05-30" version="1">MSRTSTNRSDTALELCRVSGATMGTRYTAAYYAPPGMDEAVLAERLALAVGAVDAQMSNWKADSDLSRLNRAAPGGWVPVSRNLATLLARALKIGSDTHNAFNIGVGDLVDRWGFGPSGAQCVSHPDLGEAAGASPRQPAGELIEVDERLCRVRKLGPVVLDLCGIAKGFGVDELGRVLSEHGIESWLVGIDGEMRARGNKPDGGPWAIALEAPDYERRAAMGVIELADAAIATSGDYRRWADVGGARISHTMDPRTGAPLAGGMASVTVVAKTCADADAYATALMVLGAQAGPDYARQRGLDVLFIVRDGEALRTIGTGCFADEAPSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="72" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LC85</accession>
    <name>Q1LC85_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Extra-cytoplasmic solute receptor protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">bug</name>
        <name evidence="4" type="ordered locus">Rmet_5382</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="5">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="5">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Periplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the UPF0065 (bug) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12241.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519788.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5382"/>
    <dbReference type="EnsemblBacteria" id="ABF12241">
        <property type="protein sequence ID" value="ABF12241"/>
        <property type="gene ID" value="Rmet_5382"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5382"/>
    <dbReference type="eggNOG" id="COG3181">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045683_0_0_4"/>
    <dbReference type="OMA" id="KWGPVVK"/>
    <dbReference type="OrthoDB" id="447734at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042597">
        <property type="term" value="C:periplasmic space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005064">
        <property type="entry name" value="BUG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042100">
        <property type="entry name" value="Bug_dom1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42928">
        <property type="entry name" value="PTHR42928"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03401">
        <property type="entry name" value="TctC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017082">
        <property type="entry name" value="YflP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-0675">Receptor</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004192996" evidence="3">
        <location>
            <begin position="31"/>
            <end position="331"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF12241.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="331" mass="34936" checksum="85E74256BF411EF8" modified="2006-05-30" version="1">MTFWNAARRRLLSHALISAALACGVPAAQAADSSFPTRPVRLVVGYAAGGATDVLARLVAQKMGEALGQPIVVENRAGANSNVGAEVVAKATPDGYTLYVFTIANTINATLYDKLGYDPVRDFEPVGMIAKIPNVLVVNPKLPVKTVADYVRLAKESPDGVTFASSGSGSSIHLSGEMFRSRSGANMLHVPYKGSAPAVTDLLGGQVQSMFDNAPSALPHVKSGKLRAIAVTSAQRMPQLPDVPTVAESGLPGFDVQSWFSIAAPAGTPKPVIDKLNTALNKALNASEVRERMRDLAATPEPGTPEQLRTFAASEIKRWHDVVKQSGARVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="97" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LCM2</accession>
    <name>Q1LCM2_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Transcriptional regulator, LysR family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_5245</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the LysR transcriptional regulatory family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12104.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519650.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5245"/>
    <dbReference type="EnsemblBacteria" id="ABF12104">
        <property type="protein sequence ID" value="ABF12104"/>
        <property type="gene ID" value="Rmet_5245"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5245"/>
    <dbReference type="eggNOG" id="COG0583">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039613_39_0_4"/>
    <dbReference type="OMA" id="FAYQQAW"/>
    <dbReference type="OrthoDB" id="1591366at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005119">
        <property type="entry name" value="LysR_subst-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000847">
        <property type="entry name" value="Tscrpt_reg_HTH_LysR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00126">
        <property type="entry name" value="HTH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03466">
        <property type="entry name" value="LysR_substrate"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00039">
        <property type="entry name" value="HTHLYSR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50931">
        <property type="entry name" value="HTH_LYSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH lysR-type" evidence="2">
        <location>
            <begin position="7"/>
            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF12104.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="303" mass="33206" checksum="3060813B9BFF6978" modified="2006-05-30" version="1">MDDLRRIDLNLLLTLHALLTEQHVTRAAVRLHKSQPAVSHALAQLRDIFDDPLLVRRENGLTLTARARDLLGPLDDALGQLNGLVGESTFDAAHARRCFRLALSDYGARSVLPALMRRLRREAPGIDVAVVQAGREAMLAQLADGETDLAIGVFPRLPSDIEVATLFEDHYTSVADRATLPRRGPLSVEGWLARPHVLVAMRPDAPSEIDLALAEAGMQRHVALVLPHWGAALDLVPGTDLILTVATRAVAAAPNRALRAFAPPIALAPVPFQQAWHARRRADPAHRWLRQTVWECSQQPEGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="98" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LCN2</accession>
    <name>Q1LCN2_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Rmet_5235</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="5">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="5">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12094.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5235"/>
    <dbReference type="CAZy" id="GT41">
        <property type="family name" value="Glycosyltransferase Family 41"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF12094">
        <property type="protein sequence ID" value="ABF12094"/>
        <property type="gene ID" value="Rmet_5235"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5235"/>
    <dbReference type="eggNOG" id="COG0457">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG3914">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001721_4_0_4"/>
    <dbReference type="OMA" id="HAYSEKL"/>
    <dbReference type="OrthoDB" id="370520at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029489">
        <property type="entry name" value="OGT/SEC/SPY_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_repeat"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13844">
        <property type="entry name" value="Glyco_transf_41"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="SSF48452"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0614">Plasmid</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="1">
        <location>
            <begin position="60"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="1">
        <location>
            <begin position="223"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="1">
        <location>
            <begin position="291"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="1">
        <location>
            <begin position="325"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="domain" description="Glyco_transf_41" evidence="3">
        <location>
            <begin position="366"/>
            <end position="554"/>
        </location>
    </feature>
    <feature type="domain" description="Glyco_transf_41" evidence="3">
        <location>
            <begin position="576"/>
            <end position="741"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="145"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="171"/>
            <end position="187"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF13844"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF12094.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="797" mass="86442" checksum="BFFABB897A02B452" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="99" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LD18</accession>
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            <fullName evidence="5">Transcriptional regulator, GntR family</fullName>
        </submittedName>
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        <name evidence="5" type="ordered locus">Rmet_5099</name>
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        </lineage>
    </organism>
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        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
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            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
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                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
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                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="6">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11958.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5099"/>
    <dbReference type="EnsemblBacteria" id="ABF11958">
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        <property type="gene ID" value="Rmet_5099"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5099"/>
    <dbReference type="eggNOG" id="COG1802">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017584_5_4_4"/>
    <dbReference type="OMA" id="LLHMFEA"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
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        <property type="evidence" value="ECO:0007669"/>
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    </dbReference>
    <dbReference type="CDD" id="cd07377">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.530">
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="GntR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008920">
        <property type="entry name" value="TF_FadR/GntR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000524">
        <property type="entry name" value="Tscrpt_reg_HTH_GntR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
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        <property type="entry name" value="WH_DNA-bd_sf"/>
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    </dbReference>
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    </dbReference>
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    <feature type="domain" description="HTH gntR-type" evidence="4">
        <location>
            <begin position="24"/>
            <end position="91"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABF11958.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="240" mass="27073" checksum="5E00A9172E8DB749" modified="2006-05-30" version="1">MQNGARWRRCQLPMVTNKPGLRHVTRAEAAADEIRRRILSGQYVDGYQLRQDALANELGISRIPLREALVQLESEGLVKILPHKGAIVSELSIEELTELFELRALLEPTLIRKSVKKLSVDDFKELDAILAEFSGELKAQAQDRWGDLNTMLHQLLLSKADQPKTVSIVASLLQQTDRYTRLQLSLSTASREIAEAEHRQLVDLCRKGDGRGAAALLKRHIEHAGEELRNFLLSRRRGTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="104" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LD93</accession>
    <name>Q1LD93_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_5021</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
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        <lineage>
            <taxon>Bacteria</taxon>
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            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
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        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
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    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
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                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
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        <property type="gene ID" value="Rmet_5021"/>
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    <dbReference type="KEGG" id="rme:Rmet_5021"/>
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        <property type="taxonomic scope" value="Bacteria"/>
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    <dbReference type="OMA" id="ALVIDDW"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06170">
        <property type="entry name" value="LuxR_C_like"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016032">
        <property type="entry name" value="Sig_transdc_resp-reg_C-effctor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000792">
        <property type="entry name" value="Tscrpt_reg_LuxR_C"/>
    </dbReference>
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        <property type="entry name" value="WH-like_DNA-bd_sf"/>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="HTH_LUXR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH luxR-type" evidence="2">
        <location>
            <begin position="784"/>
            <end position="848"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF11883.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="855" mass="91818" checksum="BA49BD573F8326B9" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LDB2</accession>
    <name>Q1LDB2_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Membrane efflux protein (Major facilitator superfamily MFS_1)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Rmet_5002</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="8">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="8">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11864.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519414.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5002"/>
    <dbReference type="EnsemblBacteria" id="ABF11864">
        <property type="protein sequence ID" value="ABF11864"/>
        <property type="gene ID" value="Rmet_5002"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5002"/>
    <dbReference type="eggNOG" id="COG0477">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_034180_11_2_4"/>
    <dbReference type="OMA" id="TSGKWAD"/>
    <dbReference type="OrthoDB" id="498436at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010290">
        <property type="entry name" value="TM_effector"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05977">
        <property type="entry name" value="MFS_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="SSF103473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0614">Plasmid</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="49"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="80"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="106"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="225"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="256"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="285"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="310"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="345"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="374"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="domain" description="MFS" evidence="6">
        <location>
            <begin position="1"/>
            <end position="399"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABF11864.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="441" mass="47536" checksum="03D15DBEB06B3902" modified="2006-05-30" version="1">MARTFRSLRNFNYRVWASGAIVSNIGTWMQRTAQDWLVLTELTHHNATAVGIVMSLQFGPQMLLLPLTGYAADHFNRRKLLFITQTAMGLLALGLGLMTVTGLVRLWHVYVFALLLGCVTAFDAPARQTFVSDLVGEEDLPNAVALNSTSFNGARMIGPAITGLLIASIGTGWVFLINALSFVPVIGALRMLRQHELHLKPPAQRSRGSFLEGFRYVWKRPDLKAALFMLFLIGTFGLNFPIFISTMSVSAFHAGAGQYGLLSSTMAIGSVTGTLLAARRARPRMAILVGAATIFGVGCMFGALMPNAVLFGLTLAVIGASTQTFTTSTNSLVQISTEPAMRGRVIAILLAIALGTTPLGAPVVGWVADRFGPRWALGVGAASGFAAALVGLFYLMRNRNLRFYIETGRLRYSIDKPRQTAFMAAPAASVPNVVMSKAEDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LDH7</accession>
    <name>Q1LDH7_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Siroheme decarboxylase</fullName>
            <ecNumber evidence="5">4.1.1.111</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Rmet_4937</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="8">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="8">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>2 H(+) + siroheme = 12,18-didecarboxysiroheme + 2 CO2</text>
            <dbReference type="Rhea" id="RHEA:19093"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:60052"/>
            <dbReference type="ChEBI" id="CHEBI:140497"/>
            <dbReference type="EC" id="4.1.1.111"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Porphyrin-containing compound metabolism.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the Ahb/Nir family.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.111" evidence="5"/>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11799.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519359.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4937"/>
    <dbReference type="EnsemblBacteria" id="ABF11799">
        <property type="protein sequence ID" value="ABF11799"/>
        <property type="gene ID" value="Rmet_4937"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4937"/>
    <dbReference type="eggNOG" id="COG1522">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_049427_0_0_4"/>
    <dbReference type="OMA" id="PGVSHNY"/>
    <dbReference type="OrthoDB" id="1844486at2"/>
    <dbReference type="UniPathway" id="UPA00164"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040523">
        <property type="entry name" value="AsnC_trans_reg2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17805">
        <property type="entry name" value="AsnC_trans_reg2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0456">Lyase</keyword>
    <keyword evidence="7" id="KW-0614">Plasmid</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AsnC_trans_reg2" evidence="6">
        <location>
            <begin position="64"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="domain" description="AsnC_trans_reg2" evidence="6">
        <location>
            <begin position="230"/>
            <end position="317"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023444"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023457"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF17805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABF11799.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="330" mass="37313" checksum="B22DFAD697AA539B" modified="2006-05-30" version="1">MFDDTQLYDRIQHDFPLDTRPYQRAASLLGLSERTVLSLLARDLGNGRISRIGAVFAPNSIGTSTLAALAVPPNEMKQVAERLNADPAISHNYARTGHRYNLWFVAGARDRTMLDAVLHHIADDVGYQPIDLPLEREYHIDLGFPLGKGREASKPTRRAPPVSVPKPEMDECDWRLVTALEPGLSLTPEPYHALAERCGLPLTETLQRLQRWRKNGVIRRFGVILHHRPFGYSHNAMCVWNVPDARVDAIGMRIASLPHVTLCYRRPRRLPNWQYNLFAMVHARGMHELEMTLSRFDAIGGLAAMPHAVLRASHCFKQRGTRYGNIPARV</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="102" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LDQ9</accession>
    <name>Q1LDQ9_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Histidine kinase</fullName>
            <ecNumber evidence="2">2.7.13.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Rmet_4855</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="9">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="9">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.</text>
            <dbReference type="EC" id="2.7.13.3"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.7.13.3" evidence="2"/>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11717.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519280.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4855"/>
    <dbReference type="EnsemblBacteria" id="ABF11717">
        <property type="protein sequence ID" value="ABF11717"/>
        <property type="gene ID" value="Rmet_4855"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4855"/>
    <dbReference type="eggNOG" id="COG0745">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG2205">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_114_75_4"/>
    <dbReference type="OMA" id="ACRYTER"/>
    <dbReference type="OrthoDB" id="1755994at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00082">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003661">
        <property type="entry name" value="HisK_dim/P"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036097">
        <property type="entry name" value="HisK_dim/P_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004358">
        <property type="entry name" value="Sig_transdc_His_kin-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00512">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00344">
        <property type="entry name" value="BCTRLSENSOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00388">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47384">
        <property type="entry name" value="SSF47384"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="SSF55874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="8" id="KW-0614">Plasmid</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="26"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="53"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="86"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="115"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="141"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="165"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="6">
        <location>
            <begin position="234"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="domain" description="Response regulatory" evidence="7">
        <location>
            <begin position="466"/>
            <end position="581"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="4">
        <location>
            <position position="515"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ABF11717.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="591" mass="64635" checksum="0314CBAEB8CD136A" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LDY2</accession>
    <name>Q1LDY2_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative aldo/keto reductase, oxydireductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_4782</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
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        <name>megaplasmid CH34</name>
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    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
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        <property type="protein sequence ID" value="ABF11644.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4782"/>
    <dbReference type="EnsemblBacteria" id="ABF11644">
        <property type="protein sequence ID" value="ABF11644"/>
        <property type="gene ID" value="Rmet_4782"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4782"/>
    <dbReference type="eggNOG" id="COG0656">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_064726_0_0_4"/>
    <dbReference type="OMA" id="DLLQIHN"/>
    <dbReference type="OrthoDB" id="1054546at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020471">
        <property type="entry name" value="AKR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023210">
        <property type="entry name" value="NADP_OxRdtase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036812">
        <property type="entry name" value="NADP_OxRdtase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006311">
        <property type="entry name" value="TAT_signal"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42686">
        <property type="entry name" value="PTHR42686"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00248">
        <property type="entry name" value="Aldo_ket_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51430">
        <property type="entry name" value="SSF51430"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51318">
        <property type="entry name" value="TAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193402" evidence="1">
        <location>
            <begin position="31"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="domain" description="Aldo_ket_red" evidence="2">
        <location>
            <begin position="51"/>
            <end position="289"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF11644.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="307" mass="33146" checksum="C52C69984A1D77FC" modified="2006-05-30" version="1">MSIDRRTFLGISASLAALPAGLTLGNTASAATVDAPMRRKIPGSTVQLPVIGMGTARTFNTGKSTEERAPLTEVMAVLLQRLPSAVVDTAPSYGSAEAVTGDLLQAADARRRVFLATKISANAASAPAQFASSLSDLHTDAVDLLQVHNLIDWRDNLKLLRQWKEQRKTRYIGITHYREDAQDAVAQIVRAERLDFVQINYSLGERGAERVLLPLCQERGVAVLINRPFQDGRLFAAVKGKPVPDWAAEVDCESWGQLFLKFIVSHPAVTAAIPATSNPHNMLDNAGAAFGRMPDTPERARIAAALA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-10-07" version="51" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LE36</accession>
    <name>Q1LE36_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_4728</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11590.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF11590">
        <property type="protein sequence ID" value="ABF11590"/>
        <property type="gene ID" value="Rmet_4728"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4728"/>
    <dbReference type="HOGENOM" id="CLU_2481057_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF11590.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="87" mass="10392" checksum="885C1B5C96355277" modified="2006-05-30" version="1">MTHRPKALRHWPRKCLRGDKRMLRKAVQDDSSFLEDFENAGRDWAPRLKPCGTCHACEQPVRRAELFCGITCQYSFEHFEMQLLRKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LEN4</accession>
    <name>Q1LEN4_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Spermine synthase</fullName>
            <ecNumber evidence="6">2.5.1.16</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Rmet_4527</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="7">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="7">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.16" evidence="6"/>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11392.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518985.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4527"/>
    <dbReference type="EnsemblBacteria" id="ABF11392">
        <property type="protein sequence ID" value="ABF11392"/>
        <property type="gene ID" value="Rmet_4527"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4527"/>
    <dbReference type="eggNOG" id="COG4262">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010122_1_0_4"/>
    <dbReference type="OMA" id="IFMGGMA"/>
    <dbReference type="OrthoDB" id="1613081at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004766">
        <property type="term" value="F:spermidine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006596">
        <property type="term" value="P:polyamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030374">
        <property type="entry name" value="PABS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="SSF103473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51006">
        <property type="entry name" value="PABS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2 3" id="KW-0620">Polyamine biosynthesis</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 3 6" id="KW-0808">Transferase</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="21"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="55"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="89"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="124"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="166"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="192"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="240"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="271"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="305"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="344"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="389"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="418"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="446"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="domain" description="PABS" evidence="5">
        <location>
            <begin position="464"/>
            <end position="707"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00354"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABF11392.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="844" mass="89584" checksum="D9946452D0B71B40" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="55" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LEQ2</accession>
    <name>Q1LEQ2_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Uncharacterized protein YtcA</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">Rmet_4509</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="11">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="11">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the YtcA family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11374.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4509"/>
    <dbReference type="EnsemblBacteria" id="ABF11374">
        <property type="protein sequence ID" value="ABF11374"/>
        <property type="gene ID" value="Rmet_4509"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4509"/>
    <dbReference type="eggNOG" id="ENOG50335UJ">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_157779_0_0_4"/>
    <dbReference type="OMA" id="GWAETVR"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031381">
        <property type="entry name" value="YtcA"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17090">
        <property type="entry name" value="Ytca"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0614">Plasmid</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="5 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="44"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="79"/>
            <end position="97"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008208"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00021237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ABF11374.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="102" mass="10745" checksum="251B51C703043DD2" modified="2006-05-30" version="1">MPGAFSPKTVARLGLRARMAIIGTLTCLLEGCAGAPSVGLFGAYFPLWLLSAVIGIFGAVVSYRVFVKTGWAETVRSQLIVNTAIGLIIAEIVWLIGTGHLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="118" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LEU5</accession>
    <name>Q1LEU5_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Sensor protein</fullName>
            <ecNumber evidence="5">2.7.13.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">czcS2</name>
        <name evidence="8" type="ordered locus">Rmet_4466</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="9">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="9">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Member of a two-component regulatory system.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.</text>
            <dbReference type="EC" id="2.7.13.3"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cell inner membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.7.13.3" evidence="5"/>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11331.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518933.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4466"/>
    <dbReference type="EnsemblBacteria" id="ABF11331">
        <property type="protein sequence ID" value="ABF11331"/>
        <property type="gene ID" value="Rmet_4466"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4466"/>
    <dbReference type="eggNOG" id="COG2205">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_89_6_4"/>
    <dbReference type="OMA" id="DIAMGHE"/>
    <dbReference type="OrthoDB" id="692375at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00082">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006290">
        <property type="entry name" value="CztS_silS_copS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003660">
        <property type="entry name" value="HAMP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003661">
        <property type="entry name" value="HisK_dim/P"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036097">
        <property type="entry name" value="HisK_dim/P_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004358">
        <property type="entry name" value="Sig_transdc_His_kin-like_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00672">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00512">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00344">
        <property type="entry name" value="BCTRLSENSOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00304">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00388">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47384">
        <property type="entry name" value="SSF47384"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="SSF55874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01386">
        <property type="entry name" value="cztS_silS_copS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50885">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4 5 8" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="8" id="KW-0614">Plasmid</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0902">Two-component regulatory system</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="20"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="179"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="domain" description="HAMP" evidence="7">
        <location>
            <begin position="199"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="6">
        <location>
            <begin position="260"/>
            <end position="474"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU364088"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ABF11331.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="480" mass="51404" checksum="9EB77E32DBCC90AB" modified="2006-05-30" version="1">MRLLSKLQAAMPASLTTRLALAYGLTTAAVLVGVATYLSSSLGNQLQARDEGELVGEVLLVRHLLREVGSEAEIRADTHRFADIAMGHEGLVLVVRTLGGDPLVTLNPRNERVPDLRVLPLATAPDTTQLQTWYPRNGVPSRGIVALGQIGDSGQTVEIVVLSDAGYRVALMREYHSRLLVAVLCGAIVAAALGFLLARRGLRPLRRMASDAAAVTTSRLATRLDVGSAPEELRDLAGAFNGMLARLQDSFTRLSTFSADLAHDFRTPISNLVGQTQVTLAQRRTAAEYESLLESNLEEYERLSRMIENMLFLARADNAQVALGARPLDARAELDKVAEYFEAVAADRDLTLSVTGAATVTADQPLLRRAIINLLDNALRHAPAGSTVQLAVSRGQPGHTAISVVNGGPGITPEALPHLFARFYRGDPSRRDSAKSTGLGLAIVDTIMRLHGGSVSVRSVDGQTTFELDFPDKAVSRAGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-12-02" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LEX1</accession>
    <name>Q1LEX1_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Endoribonuclease L-PSP</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_4440</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11305.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4440"/>
    <dbReference type="EnsemblBacteria" id="ABF11305">
        <property type="protein sequence ID" value="ABF11305"/>
        <property type="gene ID" value="Rmet_4440"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4440"/>
    <dbReference type="eggNOG" id="COG0251">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_100715_7_3_4"/>
    <dbReference type="OMA" id="YTAQIPI"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035959">
        <property type="entry name" value="RutC-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006175">
        <property type="entry name" value="YjgF/YER057c/UK114"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11803">
        <property type="entry name" value="PTHR11803"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01042">
        <property type="entry name" value="Ribonuc_L-PSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55298">
        <property type="entry name" value="SSF55298"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF11305.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="140" mass="15088" checksum="E32828162C5A972B" modified="2006-05-30" version="1">MPGQHRFPSHRYPQHTMKQIIDTGLPRSAAPVEWAVLGNGILFTTQIPTGADGNVVEGGMEAQARQTLQNLKQTLDAAGGSLADLTQVIVYVTDRADLAVFNRVYAEMIPAPYPNRAAIVTVGFGRPEMLVEILAYAALG</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LEX6</accession>
    <name>Q1LEX6_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Nitrilase/N-carbamoyl-D-aminoacid amidohydrolase</fullName>
            <ecNumber evidence="3">3.5.-.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">nit</name>
        <name evidence="3" type="ordered locus">Rmet_4435</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EC" id="3.5.-.-" evidence="3"/>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11300.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4435"/>
    <dbReference type="EnsemblBacteria" id="ABF11300">
        <property type="protein sequence ID" value="ABF11300"/>
        <property type="gene ID" value="Rmet_4435"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4435"/>
    <dbReference type="eggNOG" id="COG0388">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030130_1_2_4"/>
    <dbReference type="OMA" id="LFDSGYC"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016811">
        <property type="term" value="F:hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006807">
        <property type="term" value="P:nitrogen compound metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07572">
        <property type="entry name" value="nit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.110.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003010">
        <property type="entry name" value="C-N_Hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036526">
        <property type="entry name" value="C-N_Hydrolase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045254">
        <property type="entry name" value="Nit1/2_C-N_Hydrolase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00795">
        <property type="entry name" value="CN_hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56317">
        <property type="entry name" value="SSF56317"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50263">
        <property type="entry name" value="CN_HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CN hydrolase" evidence="2">
        <location>
            <begin position="4"/>
            <end position="249"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF11300.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="278" mass="29908" checksum="6EB9C7E8F6474779" modified="2006-05-30" version="1">MMRIKVAAIQIDSRQDREANLAALEHWILAAASDGAKLIVTPEYSDVRGDANALQAAASAVPGPVSEHISSLAQRTGCWIHLGSMHERLPGETRLGNSGITFAPDGGIAARYRKVHLYDAVVNGKPYRESADFAPGDGLHTVDAAGLTLGLSICYDLRFGELYRTLRARGANVLLVPAAFNVHTGRDHWETLLRARAIENQCYVIAAAQIGGPGPALPCLGRSMIIDPWGTVLACMPDTTGFITAELDTERVAGLREALPAWEHRRTDLYFGGIQNSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LF02</accession>
    <name>Q1LF02_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Taurine dioxygenase, 2-oxoglutarate-dependent</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">tauD</name>
        <name evidence="3" type="ordered locus">Rmet_4409</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11274.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518888.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4409"/>
    <dbReference type="EnsemblBacteria" id="ABF11274">
        <property type="protein sequence ID" value="ABF11274"/>
        <property type="gene ID" value="Rmet_4409"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4409"/>
    <dbReference type="eggNOG" id="COG2175">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036005_2_1_4"/>
    <dbReference type="OMA" id="LRWKVVF"/>
    <dbReference type="OrthoDB" id="1742732at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051213">
        <property type="term" value="F:dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.130.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042098">
        <property type="entry name" value="TauD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003819">
        <property type="entry name" value="TauD/TfdA-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02668">
        <property type="entry name" value="TauD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TauD" evidence="2">
        <location>
            <begin position="28"/>
            <end position="294"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF11274.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="301" mass="34527" checksum="EC938623316F5F98" modified="2006-05-30" version="1">MRTSDTKDTKDTKNAEDTRAWPFTLHRCTPTIGAEVSGVDLGNLDDTTYRALRQALLDHKVLFFRDQHITPAQHVAAARRFGELEVHPMIGHHPDHPELVVFGRGGDKRGRENLYHSDVSWRELPSMGSMLRCVECPEIGGDTIWVNMAAAYAALPEDVKTRIAELRAVHDAMPTFGQSMDGERYDEMRAKYPPMVHPVVRTHPETGEKILYVNEGFTTHFANYARMQPYRIGSDFRMAEMDLMQYLFRQAAAPEYQVRLRWRANTVALWDNRSTQHYAVQDYFPAVRHMNRATIIGDRPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LF67</accession>
    <name>Q1LF67_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Porin, Gram-negative type</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Rmet_4344</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="8">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="8">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11209.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518831.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4344"/>
    <dbReference type="EnsemblBacteria" id="ABF11209">
        <property type="protein sequence ID" value="ABF11209"/>
        <property type="gene ID" value="Rmet_4344"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4344"/>
    <dbReference type="eggNOG" id="COG3203">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038238_2_0_4"/>
    <dbReference type="OMA" id="ALFQHAN"/>
    <dbReference type="OrthoDB" id="1064531at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046930">
        <property type="term" value="C:pore complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015288">
        <property type="term" value="F:porin activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006811">
        <property type="term" value="P:ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00342">
        <property type="entry name" value="gram_neg_porins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.160.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033900">
        <property type="entry name" value="Gram_neg_porin_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023614">
        <property type="entry name" value="Porin_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002299">
        <property type="entry name" value="Porin_Neis"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13609">
        <property type="entry name" value="Porin_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00184">
        <property type="entry name" value="NEISSPPORIN"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0406">Ion transport</keyword>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-0626">Porin</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1134">Transmembrane beta strand</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193089" evidence="5">
        <location>
            <begin position="21"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="domain" description="Porin_4" evidence="6">
        <location>
            <begin position="7"/>
            <end position="334"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF13609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABF11209.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="371" mass="39012" checksum="2440DC9D67576FF3" modified="2006-05-30" version="1">MKCKYLAIAVAMLTASSVQAQSSVTLYGVVDAGIEYLNHAVASTGTVAPGAKGHSLVALQSGNQSGSRWGLRGIEDLGGGLRSLFVLESGFSIDTGTSTQSGRLFGRGAYVGLENQWGRLTLGRHTVPFYDFAVTYDPMAISSRYAIGAQDPFMGAARADNSVKYMGTFGGLSVTGLYSFNYNNQEVPGNFTNGREYSLGANYSGGSFSIGAVYDQINQSAATALQTNSLTQRAAVAGTYAYGNAKVYAGYRYAHAFNGASLPGSTVANTASNLAWAGVGYQLTPALSFAGSAYYQNLRNSRSGNPWQFVMVADYALSKRTDLYTAASYVLNKGKSSLGVNGFNSQLGTTSEAVQPGANQFGAVVGVRHKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="109" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LF91</accession>
    <name>Q1LF91_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Transcriptional regulator, AraC family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_4320</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11185.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518809.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4320"/>
    <dbReference type="EnsemblBacteria" id="ABF11185">
        <property type="protein sequence ID" value="ABF11185"/>
        <property type="gene ID" value="Rmet_4320"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4320"/>
    <dbReference type="eggNOG" id="COG2207">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_049704_5_0_4"/>
    <dbReference type="OMA" id="MLHVLMD"/>
    <dbReference type="OrthoDB" id="1785082at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035418">
        <property type="entry name" value="AraC-bd_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018060">
        <property type="entry name" value="HTH_AraC"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14525">
        <property type="entry name" value="AraC_binding_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12833">
        <property type="entry name" value="HTH_18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00342">
        <property type="entry name" value="HTH_ARAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01124">
        <property type="entry name" value="HTH_ARAC_FAMILY_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH araC/xylS-type" evidence="2">
        <location>
            <begin position="219"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="310"/>
            <end position="333"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS01124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF11185.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="333" mass="36246" checksum="C98C9CE4F2FDB4B5" modified="2006-05-30" version="1">MRAQSPLLIRNWSTSDVPQSQRYDYYAHALATAIFPIQLSGSAPSAFGVDMSAADLGGMTVIRQRGTPHRCFADRGNIARAEGRTFHLLTSTRSAWTLEHLTRERLGPGDVIMCDSSIPFNLEIGNDFNIVHVALSEAWIRQWLPAPSVLVGARIDANDSWGRALSAFLQSLEPARLADYPLPRSFIGDHIGSLLAFLAQSRSAVPPKPAKTEGAAVLDRIRDCMAGQSREATLCAADVAAAVSISVRSLHRHFARSSTTFGAVLMAMRCADAVRMLESSSFSRLTVAEIGRRAGFADAAHFSRVMRARVGHTPNQLRRAHAGPSSRDDPKEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="98" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LFA3</accession>
    <name>Q1LFA3_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Transcriptional regulator, TetR family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Rmet_4307</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="5">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="5">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11173.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518798.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4307"/>
    <dbReference type="EnsemblBacteria" id="ABF11173">
        <property type="protein sequence ID" value="ABF11173"/>
        <property type="gene ID" value="Rmet_4307"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4307"/>
    <dbReference type="eggNOG" id="COG1309">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069356_28_0_4"/>
    <dbReference type="OMA" id="MAVQATN"/>
    <dbReference type="OrthoDB" id="1204683at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023772">
        <property type="entry name" value="DNA-bd_HTH_TetR-type_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001647">
        <property type="entry name" value="HTH_TetR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036271">
        <property type="entry name" value="Tet_transcr_reg_TetR-rel_C_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00440">
        <property type="entry name" value="TetR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48498">
        <property type="entry name" value="SSF48498"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01081">
        <property type="entry name" value="HTH_TETR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50977">
        <property type="entry name" value="HTH_TETR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0614">Plasmid</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0678">Repressor</keyword>
    <feature type="domain" description="HTH tetR-type" evidence="3">
        <location>
            <begin position="6"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="H-T-H motif" evidence="2">
        <location>
            <begin position="29"/>
            <end position="48"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF11173.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="200" mass="21869" checksum="B5A491C043034F90" modified="2006-05-30" version="1">MARPRQFDAQTALSAAQDAFWAKGYNATSTRELTQAMGLTQPSLYNAFGDKRTLFLQALEDYLNHTLRERIARLEGTLPPAQALEAFFAESIERGVSDPQHRGCMLINTALDASADDPELAAIVAEELTTLGHFFERTIRAGQADGTVDPTLAARDVSATLLSVQIGLRVMSRVMPDRALLEASVRPVLALLERKPRGTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LFB9</accession>
    <name>Q1LFB9_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">2,3-dimethylmalate lyase</fullName>
            <ecNumber evidence="1">2.7.8.23</ecNumber>
            <ecNumber evidence="1">4.1.3.30</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">prpB</name>
        <name evidence="1" type="ordered locus">Rmet_4291</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EC" id="2.7.8.23" evidence="1"/>
    <dbReference type="EC" id="4.1.3.30" evidence="1"/>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11157.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518782.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4291"/>
    <dbReference type="EnsemblBacteria" id="ABF11157">
        <property type="protein sequence ID" value="ABF11157"/>
        <property type="gene ID" value="Rmet_4291"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4291"/>
    <dbReference type="eggNOG" id="COG2513">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027389_3_2_4"/>
    <dbReference type="OMA" id="QMIANID"/>
    <dbReference type="OrthoDB" id="1485205at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008807">
        <property type="term" value="F:carboxyvinyl-carboxyphosphonate phosphorylmutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046421">
        <property type="term" value="F:methylisocitrate lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd00377">
        <property type="entry name" value="ICL_PEPM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039556">
        <property type="entry name" value="ICL/PEPM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015813">
        <property type="entry name" value="Pyrv/PenolPyrv_Kinase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040442">
        <property type="entry name" value="Pyrv_Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51621">
        <property type="entry name" value="SSF51621"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0456">Lyase</keyword>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF11157.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="295" mass="31832" checksum="DCED3C0CD687DDB8" modified="2006-05-30" version="1">MTTLSAARRAAFRAKVNERRGLLVAGAFNAMSARVVEETGFEALYLTGAGVTNMSLGLPDLGFIGLHEIAEHTARVRDAVALPLIVDADTGFGNALNVRHTVRTLERSGADAIQIEDQVMPKKCGHFSGKEVIATSEMLGKIRAAVDAREDPNLLIMARTDAAAVHGMEAAIERGHRFIEAGADILFIEATESLADVERLPKLIAAPQLINIVIGGKTPVQSRETLASHGYALVLYANATLQGAVLGMQRALSTLRTNGRLDEDATLVAPFSERQRLVNKPLYDRLDREYAANDH</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="98" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LFC0</accession>
    <name>Q1LFC0_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Transcriptional regulator, AraC family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Rmet_4290</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="6">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="6">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11156.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518781.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4290"/>
    <dbReference type="EnsemblBacteria" id="ABF11156">
        <property type="protein sequence ID" value="ABF11156"/>
        <property type="gene ID" value="Rmet_4290"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4290"/>
    <dbReference type="eggNOG" id="COG2207">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047522_1_1_4"/>
    <dbReference type="OMA" id="TVNEGMW"/>
    <dbReference type="OrthoDB" id="1622875at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032687">
        <property type="entry name" value="AraC-type_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018060">
        <property type="entry name" value="HTH_AraC"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12625">
        <property type="entry name" value="Arabinose_bd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12833">
        <property type="entry name" value="HTH_18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00342">
        <property type="entry name" value="HTH_ARAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01124">
        <property type="entry name" value="HTH_ARAC_FAMILY_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0614">Plasmid</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH araC/xylS-type" evidence="4">
        <location>
            <begin position="234"/>
            <end position="332"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS01124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABF11156.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="336" mass="38137" checksum="E7838F9FC86E7C21" modified="2006-05-30" version="1">MRRSQTFSLDIGWQPLLKDFGLRPDQILRRAGLPEDMLSRGERGLTTDEWFRFWRALETEAADTMFPLRIVEVIAAESFDPPLFAALCSANLMQAVQRLAKYKQLVAPMSLEVDVGTSSELTVSPRWLSIQADVPYSLQVAEIAFFLRLTRLGTREPIKALRVVLPQLPPNAYVRRFEKFFGAPVTPGESPSVTFSAEDALRPFLTVNEGMWRVFEPDLRRRLSELDATAATAERVRAVLLELLPSNAATIEKAAERLGMSKRTLQRRLEDEGENFRALVNATRERLARHYLGNTKMSSGEIAFLLGFEDPNSFYRAFQEWTGQTPDSARRAMHLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="99" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LFK7</accession>
    <name>Q1LFK7_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Transcriptional regulator, LysR family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_4202</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the LysR transcriptional regulatory family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11069.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518692.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4202"/>
    <dbReference type="EnsemblBacteria" id="ABF11069">
        <property type="protein sequence ID" value="ABF11069"/>
        <property type="gene ID" value="Rmet_4202"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4202"/>
    <dbReference type="eggNOG" id="COG0583">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039613_6_4_4"/>
    <dbReference type="OMA" id="CIGYLEY"/>
    <dbReference type="OrthoDB" id="1439189at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005119">
        <property type="entry name" value="LysR_subst-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000847">
        <property type="entry name" value="Tscrpt_reg_HTH_LysR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00126">
        <property type="entry name" value="HTH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03466">
        <property type="entry name" value="LysR_substrate"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00039">
        <property type="entry name" value="HTHLYSR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50931">
        <property type="entry name" value="HTH_LYSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH lysR-type" evidence="2">
        <location>
            <begin position="1"/>
            <end position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF11069.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="295" mass="32178" checksum="8420E8362F4097B9" modified="2006-05-30" version="1">MDFRQLRYFVAVAEALSFSEAARRLHVSQPPLSIQIKALEDELGSPLLERSRHHVALTPAGELFLEKARAALANLDEARDVVRQVASGEAGEIRVGFTASVPMQDVFPRAVHAFREAHRHARLELVHMSTGQQLQQLESGEIDIGFLRPSPQFRPPARIEVREVLADRLVAVLPLGHLLARSNEQTEIDISELSGERFLLFPRGLGCGLFDHVTTLCNRAGFAPNVAQEAREATTIIGLVASGAGVSVLPAIYARTGIPGVAYRAIAGPDAGSRILMAHRADALSPIMKRFVGYF</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LFV6</accession>
    <name>Q1LFV6_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">N-ethylmaleimide reductase, FMN-linked</fullName>
            <ecNumber evidence="2">1.-.-.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">nemA</name>
        <name evidence="2" type="ordered locus">Rmet_4103</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EC" id="1.-.-.-" evidence="2"/>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10970.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518598.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4103"/>
    <dbReference type="EnsemblBacteria" id="ABF10970">
        <property type="protein sequence ID" value="ABF10970"/>
        <property type="gene ID" value="Rmet_4103"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4103"/>
    <dbReference type="eggNOG" id="COG1902">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012153_0_0_4"/>
    <dbReference type="OMA" id="RWFIANP"/>
    <dbReference type="OrthoDB" id="1064766at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001155">
        <property type="entry name" value="OxRdtase_FMN_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045247">
        <property type="entry name" value="Oye-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22893">
        <property type="entry name" value="PTHR22893"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00724">
        <property type="entry name" value="Oxidored_FMN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Oxidored_FMN" evidence="1">
        <location>
            <begin position="13"/>
            <end position="345"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00724"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF10970.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="369" mass="40471" checksum="D6CE9C6581401DAE" modified="2006-05-30" version="1">MGAQTEAFLTEALFQPIKLGRIELANRMAMAPLTRSRADDDLVPTEMVVEYYRQRASVGLIIAEATQVSETAQGYTNTPGVYTPEQIAAWKTVTDAVHAQGGKIFLQIWHTGRMSHSHFQPDNQAPVAPSAIAANAKTYINGQGYVECSLPRALEASEIPEIVNDFRVAAANAIKAGFDGVEVHGAHGYLLDAFLRDGTNKRTDAYGGSIENRARFLLEVMAAVTAEIGADRVGVRLAPVSPVNDAVESNPQPLFEYVVRELEKLHPVYIHVVEGYTGGPRDNVPFDYEALHRLYSGVWMVNNGYSKEMAEEAIRAGRADMVSFGRKMITNPDLPRRFRENQPLNSPFEDASLYGGTGPHGYVDYPALA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LFZ3</accession>
    <name>Q1LFZ3_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative periplasmic nitrate reductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">napE</name>
        <name evidence="2" type="ordered locus">Rmet_4065</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10933.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518560.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4065"/>
    <dbReference type="EnsemblBacteria" id="ABF10933">
        <property type="protein sequence ID" value="ABF10933"/>
        <property type="gene ID" value="Rmet_4065"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4065"/>
    <dbReference type="eggNOG" id="COG4459">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_200276_2_0_4"/>
    <dbReference type="OMA" id="WMSQLIS"/>
    <dbReference type="OrthoDB" id="2017834at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010649">
        <property type="entry name" value="NapE_TorE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004448">
        <property type="entry name" value="Nitrate_reductase_NapE"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06796">
        <property type="entry name" value="NapE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02973">
        <property type="entry name" value="nitrate_rd_NapE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="44"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF10933.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="54" mass="5950" checksum="83D8455AE65286FD" modified="2006-05-30" version="1">MQSGSVEDPQRKTEELRSFLFLTAVMVPVLSVIVVAGYGFIVWMSQLISGPPTH</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="60" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LG38</accession>
    <name>Q1LG38_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Nicotinamide riboside transporter PnuC</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">Rmet_4020</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="10">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="10">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Required for nicotinamide riboside transport across the inner membrane.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the nicotinamide ribonucleoside (NR) uptake permease (TC 4.B.1) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10888.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008648027.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF10888">
        <property type="protein sequence ID" value="ABF10888"/>
        <property type="gene ID" value="Rmet_4020"/>
    </dbReference>
    <dbReference type="GeneID" id="60823390"/>
    <dbReference type="KEGG" id="rme:Rmet_4020"/>
    <dbReference type="HOGENOM" id="CLU_182857_0_0_4"/>
    <dbReference type="OMA" id="WIGCAFG"/>
    <dbReference type="OrthoDB" id="1845941at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034257">
        <property type="term" value="F:nicotinamide riboside transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006419">
        <property type="entry name" value="NMN_transpt_PnuC"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04973">
        <property type="entry name" value="NMN_transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0614">Plasmid</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="13"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="38"/>
            <end position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002672"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006669"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00017522"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ABF10888.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="98" mass="10688" checksum="01CFFD2A666537E1" modified="2006-05-30" version="1">MELAAQIPNLLNGLEWSGCALAMSGSFLVALHNRASRWGWIAYICSNLVWSAFALATGTYGLFVQQLGFTATSLYGLYRHTRYYRELAAHTPTAAAAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="52" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LH83</accession>
    <name>Q1LH83_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_3621</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10493.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518152.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF10493">
        <property type="protein sequence ID" value="ABF10493"/>
        <property type="gene ID" value="Rmet_3621"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3621"/>
    <dbReference type="HOGENOM" id="CLU_2286824_0_0_4"/>
    <dbReference type="OMA" id="APPHWER"/>
    <dbReference type="OrthoDB" id="1764702at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="28"/>
            <end position="42"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF10493.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="107" mass="12134" checksum="C2648AD17244E6C0" modified="2006-05-30" version="1">MQLDFARAPTSPATDLARRLAPAVTKPTPRRRKKEAPPHWERGYRSHYYRNERGDKLGEVRLSDRGDLPVVYHWAAGNYSGAEGSLAHARARVEETVGFGMRQLSLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LHA5</accession>
    <name>Q1LHA5_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Short-chain dehydrogenase/reductase SDR</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_3599</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10471.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518014.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3599"/>
    <dbReference type="EnsemblBacteria" id="ABF10471">
        <property type="protein sequence ID" value="ABF10471"/>
        <property type="gene ID" value="Rmet_3599"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3599"/>
    <dbReference type="eggNOG" id="COG0300">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010194_2_1_4"/>
    <dbReference type="OMA" id="MDYADLE"/>
    <dbReference type="OrthoDB" id="1582456at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00061">
        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU000363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF10471.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="296" mass="31608" checksum="EB82B0A67B49D3FE" modified="2006-05-30" version="1">MKDFHNKVAAITGAGSGMGRCLAIQLAQSGCHVAISDCNEAGLAQTVAEVRRVAPMVQVTSHRVDVADRAAVHAWADDVARAHGRVNLIFNNAGVALSSTIEGMAYDDLEWIIGINFWGVVYGTKAFLPHLKASGEGHVVNTSSIFGLFAQPGMGAYNASKYAVRGFTESLRQELDLMDCGVSATTVHPGGIKTNIARASRVSSSVNGLLVRDAQQGREEFEKFFITTADKAARVILDGVRANKRRVLIGPDAYAADALSRVLPAAYQSLVVRDTRRKSKRLLSDTAGAPARGERA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="96" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LHJ4</accession>
    <name>Q1LHJ4_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Leucine/isoleucine/valine transporter subunit, ATP-binding component of ABC superfamily</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">livF11</name>
        <name evidence="9" type="ordered locus">Rmet_3510</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10382.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517934.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3510"/>
    <dbReference type="EnsemblBacteria" id="ABF10382">
        <property type="protein sequence ID" value="ABF10382"/>
        <property type="gene ID" value="Rmet_3510"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3510"/>
    <dbReference type="eggNOG" id="COG0410">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_2_4"/>
    <dbReference type="OMA" id="TGDMAME"/>
    <dbReference type="OrthoDB" id="1475841at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 7 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="1" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-1278">Translocase</keyword>
    <feature type="domain" description="ABC transporter" evidence="8">
        <location>
            <begin position="5"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="37"/>
            <end position="44"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ABF10382.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="247" mass="26621" checksum="383D80729509424D" modified="2006-05-30" version="1">MSLILEVKDLHVRYGKVEAVHGANLKVEAGKIVTVIGPNGAGKSTMLNAVMGALPVSGSSTGSVRFLGHDMAGIPVEGRVARGMCLVPEKRELFASMTVEDNLLLGAFRRKRAGERNYLDQKDVVYDLFPRLRERAKQEAGTLSGGERQMLAVGRALMAKPQLLMLDEPSLGLAPLIVKEIFHIIHNLRQTGVATLLIEQNARAALQVADYGYVIETGDMAMEGEASALASNPKVIETYLGLTKKAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="111" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LHM7</accession>
    <name>Q1LHM7_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">DNA-binding response regulator in two-component regulatory system with EnvZ</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">ompR</name>
        <name evidence="6" type="ordered locus">Rmet_3477</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10349.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3477"/>
    <dbReference type="EnsemblBacteria" id="ABF10349">
        <property type="protein sequence ID" value="ABF10349"/>
        <property type="gene ID" value="Rmet_3477"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3477"/>
    <dbReference type="eggNOG" id="COG0745">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_30_4_4"/>
    <dbReference type="OMA" id="FSLCRWV"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00383">
        <property type="entry name" value="trans_reg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001867">
        <property type="entry name" value="OmpR/PhoB-type_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016032">
        <property type="entry name" value="Sig_transdc_resp-reg_C-effctor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039420">
        <property type="entry name" value="WalR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48111">
        <property type="entry name" value="PTHR48111"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00486">
        <property type="entry name" value="Trans_reg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00862">
        <property type="entry name" value="Trans_reg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46894">
        <property type="entry name" value="SSF46894"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51755">
        <property type="entry name" value="OMPR_PHOB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 3 6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Response regulatory" evidence="4">
        <location>
            <begin position="28"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="domain" description="OmpR/PhoB-type" evidence="5">
        <location>
            <begin position="157"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="OmpR/PhoB-type" evidence="3">
        <location>
            <begin position="157"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="2">
        <location>
            <position position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABF10349.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="261" mass="29036" checksum="39F361FB7E7A8DDA" modified="2006-05-30" version="1">MGAPSGRGSAAVPIRKLAMDLSPALPIRLLIVDDDPQIRAMLAEYLATFGMEAEGAEGGTAMRAAMSKQEYDLVILDLSLPGENGLTLCREIRESSNLPVIMLTARAELADRVVGLEVGADDYVTKPFEMRELVARIHTVLRRSRADVRRPAPAAPGHEVRFAAWRLNTTLRQLVDAEDTVVPLSNAEFRLLLTFIEHPNRVLDREMLINTARGRDLDVFDRSIDLLVSRLRQKLRDDPRDSALIRTVRGEGYVFTGTVEH</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="68" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LI03</accession>
    <name>Q1LI03_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">NYN domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_3351</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10223.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3351"/>
    <dbReference type="EnsemblBacteria" id="ABF10223">
        <property type="protein sequence ID" value="ABF10223"/>
        <property type="gene ID" value="Rmet_3351"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3351"/>
    <dbReference type="eggNOG" id="COG1432">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1097832_0_0_4"/>
    <dbReference type="OMA" id="NCCDEIL"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004540">
        <property type="term" value="F:ribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021139">
        <property type="entry name" value="NYN_MARF1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01936">
        <property type="entry name" value="NYN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NYN" evidence="1">
        <location>
            <begin position="21"/>
            <end position="164"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF10223.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="253" mass="28598" checksum="18B8C82FCF4546E6" modified="2006-05-30" version="1">MILSVQLATAANAMKKQVLNIAVYVDMENVASIDFALEDLMSALLLAEDDHNCIFVIKAAYGSQGNAKKALKEQLIDHNFTLIDTPKIGVEKNRADLCISLDAFETLYLGNPRIDRYCFLTSDSDFTVIGDRLRKYGKEVWLACRRKDKDRAILAKAFDTMLFLEDFAQAKIRTFDDAIENLFVKALRNIERSKLPVNVSTANNKMKELDPSFDVSRTIYKNFMPLVKEMASRGHLRFITSPGGENRITDILS</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LI08</accession>
    <name>Q1LI08_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Universal stress protein, UspA family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">uspA8</name>
        <name evidence="3" type="ordered locus">Rmet_3346</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal stress protein A family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10218.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517790.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3346"/>
    <dbReference type="EnsemblBacteria" id="ABF10218">
        <property type="protein sequence ID" value="ABF10218"/>
        <property type="gene ID" value="Rmet_3346"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3346"/>
    <dbReference type="eggNOG" id="COG0589">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_049301_11_0_4"/>
    <dbReference type="OMA" id="EGIPHKT"/>
    <dbReference type="OrthoDB" id="2040681at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006015">
        <property type="entry name" value="Universal_stress_UspA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006016">
        <property type="entry name" value="UspA"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00582">
        <property type="entry name" value="Usp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01438">
        <property type="entry name" value="UNVRSLSTRESS"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Usp" evidence="2">
        <location>
            <begin position="6"/>
            <end position="150"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008791"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF10218.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="156" mass="16097" checksum="4C1AE65EC0DA239A" modified="2006-05-30" version="1">MASKAYSKIVVAVDGSSTSDLALQEAIRVAGGSGATILALYVVDNAMVLFDAGYYDPSQIERAFVESGTRALEAASKLLGDAGVKFETQLVTEPAVAGDIAGSINAAASEWGGDLLVIGTHGRRGVRRLVLGSVAEAVIRQSTMPVLLVRGTSSEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="104" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LI32</accession>
    <name>Q1LI32_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transcriptional regulator, AraC-family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_3322</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10194.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517773.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3322"/>
    <dbReference type="EnsemblBacteria" id="ABF10194">
        <property type="protein sequence ID" value="ABF10194"/>
        <property type="gene ID" value="Rmet_3322"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3322"/>
    <dbReference type="eggNOG" id="COG2207">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047930_0_1_4"/>
    <dbReference type="OMA" id="WSADNDQ"/>
    <dbReference type="OrthoDB" id="1785082at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035418">
        <property type="entry name" value="AraC-bd_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018060">
        <property type="entry name" value="HTH_AraC"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14525">
        <property type="entry name" value="AraC_binding_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12833">
        <property type="entry name" value="HTH_18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00342">
        <property type="entry name" value="HTH_ARAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01124">
        <property type="entry name" value="HTH_ARAC_FAMILY_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH araC/xylS-type" evidence="1">
        <location>
            <begin position="233"/>
            <end position="333"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS01124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF10194.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="337" mass="37173" checksum="EB91C9CA02B9EB24" modified="2006-05-30" version="1">MPQMPPTAPAMVPLAGALLHDTGRLVFDSDDLDTTRARVASVFKPHRLTLHGNALRARMHHVPVGAVSFNRLSYGGDVTIDPGPLGDFLLIQMPMAGQADIHCGGQHVVSDSECASVLTPSEPLRMRWSADNDQLIVRIERSALERVCAAYLGRRPNQPLRFQLGMAWRNTGWQSLVQYLADTLNHAPQTASHPLTACQLEQLAIGTLLTFQPHSLSDVLREHGKPLAPRHVKVVEEYIHAHADAAVTPALLAEMAGVSLRSLYAGFREHRGLSPMTYLRTVRLERVRQDLLNDASLSSVTAAALRWGFAHLGRFSVEYRRAFGECPGETLRRRGRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LI36</accession>
    <name>Q1LI36_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative flavin reductase (Conserved protein, domain typically associated with flavoprotein oxygenases)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_3318</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10190.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517769.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3318"/>
    <dbReference type="EnsemblBacteria" id="ABF10190">
        <property type="protein sequence ID" value="ABF10190"/>
        <property type="gene ID" value="Rmet_3318"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3318"/>
    <dbReference type="eggNOG" id="COG1853">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_059021_1_0_4"/>
    <dbReference type="OMA" id="FMVGRTS"/>
    <dbReference type="OrthoDB" id="1681849at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016646">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-NH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.110.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002563">
        <property type="entry name" value="Flavin_Rdtase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012349">
        <property type="entry name" value="Split_barrel_FMN-bd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01613">
        <property type="entry name" value="Flavin_Reduct"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00903">
        <property type="entry name" value="Flavin_Reduct"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Flavin_Reduct" evidence="1">
        <location>
            <begin position="24"/>
            <end position="167"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00903"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF10190.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="170" mass="17959" checksum="E154B58682AF7477" modified="2006-05-30" version="1">MDSTKIVASTPAGAPDPRALRRVCGRFATGVAIIGACAPQGRPVGLTVNSFASLSLDPPLIVWSLARHSPNAALFQSGEAFGVSILRAGHDELARRFARPQPDKFAGVPHWRCPRGVPYLDEALATLSCRVERADPLGDHLLIVGAVEDFAASDGDPLVFYGGDFVRVAA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2007-09-11" modified="2022-05-25" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LI59</accession>
    <name>RL36_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">50S ribosomal protein L36</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpmJ</name>
        <name type="ordered locus">Rmet_3295</name>
    </gene>
    <organism>
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the bacterial ribosomal protein bL36 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10167.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008642959.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q1LI59"/>
    <dbReference type="SMR" id="Q1LI59"/>
    <dbReference type="STRING" id="266264.Rmet_3295"/>
    <dbReference type="EnsemblBacteria" id="ABF10167">
        <property type="protein sequence ID" value="ABF10167"/>
        <property type="gene ID" value="Rmet_3295"/>
    </dbReference>
    <dbReference type="GeneID" id="60826622"/>
    <dbReference type="KEGG" id="rme:Rmet_3295"/>
    <dbReference type="eggNOG" id="COG0257">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_135723_6_2_4"/>
    <dbReference type="OrthoDB" id="2066169at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00251">
        <property type="entry name" value="Ribosomal_L36"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000473">
        <property type="entry name" value="Ribosomal_L36"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035977">
        <property type="entry name" value="Ribosomal_L36_sp"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42888">
        <property type="entry name" value="PTHR42888"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00444">
        <property type="entry name" value="Ribosomal_L36"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57840">
        <property type="entry name" value="SSF57840"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01022">
        <property type="entry name" value="rpmJ_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00828">
        <property type="entry name" value="RIBOSOMAL_L36"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <feature type="chain" id="PRO_0000302279" description="50S ribosomal protein L36">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00251"/>
        </source>
    </evidence>
    <sequence length="38" mass="4378" checksum="29FB999241065455" modified="2006-05-30" version="1">MKVLASVKRICRNCKIIKRNGVVRVICSSDPRHKQRQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LIV3</accession>
    <name>Q1LIV3_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_3051</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09923.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517554.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3051"/>
    <dbReference type="EnsemblBacteria" id="ABF09923">
        <property type="protein sequence ID" value="ABF09923"/>
        <property type="gene ID" value="Rmet_3051"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3051"/>
    <dbReference type="eggNOG" id="COG0697">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033863_5_0_4"/>
    <dbReference type="OMA" id="GLNWPVM"/>
    <dbReference type="OrthoDB" id="1515911at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000620">
        <property type="entry name" value="EamA_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00892">
        <property type="entry name" value="EamA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="35"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="67"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="97"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="123"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="151"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="182"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="213"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="241"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="268"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="domain" description="EamA" evidence="2">
        <location>
            <begin position="6"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="domain" description="EamA" evidence="2">
        <location>
            <begin position="152"/>
            <end position="284"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF09923.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="294" mass="31886" checksum="5262C0CC9C707AA9" modified="2006-05-30" version="1">MNLDRRGLILLVILTLAWGINWPIMKLGVAHFPAMAFRLLCMAGGLVALGLALTFRGDSLRVPRAAWGTVLKLAIPNMVIWHLFAICAVKMLSSGRAAILGYTMPIWAVVWGLVIFRDRIALSAWIGIGCALAGTVLLLSGEIAALTGSPAGTLLMLVAAAGWGFGTQLMKRTHTDVPIGAMTFWMLAITLPFLGAGTLLFESGWRMPTAIEWGAIAYNAVVVFAYCHLVWFALARSLPPVVSSLSIMFIPVVGVFSGMWFLGETPHWQDYVAIVLMFAALSSVMLAPLLRRRM</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="60" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LIY1</accession>
    <name>Q1LIY1_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Regulator of plasmid copy number (Ribbon-helix-helix)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">parG</name>
        <name evidence="2" type="ordered locus">Rmet_3022</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09895.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_003056186.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3022"/>
    <dbReference type="EnsemblBacteria" id="ABF09895">
        <property type="protein sequence ID" value="ABF09895"/>
        <property type="gene ID" value="Rmet_3022"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3022"/>
    <dbReference type="eggNOG" id="ENOG5032YZW">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_185285_0_0_4"/>
    <dbReference type="OMA" id="TREFPNT"/>
    <dbReference type="OrthoDB" id="1917231at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010985">
        <property type="entry name" value="Ribbon_hlx_hlx"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47598">
        <property type="entry name" value="SSF47598"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF09895.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="87" mass="9475" checksum="A2AF2A845554E1E8" modified="2006-05-30" version="1">MSTPASKRTGKRIGIGARPPANPHAEAWIRQGSADDLQKGDLYTARLTLDITPAMRARIKVSAFTQGVTVADLLRALLEREFPEKSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LJ84</accession>
    <name>Q1LJ84_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="9">ABC-type transporter, ATPase component</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">Rmet_2919</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09792.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517465.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2919"/>
    <dbReference type="EnsemblBacteria" id="ABF09792">
        <property type="protein sequence ID" value="ABF09792"/>
        <property type="gene ID" value="Rmet_2919"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2919"/>
    <dbReference type="eggNOG" id="COG1122">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_22_4"/>
    <dbReference type="OMA" id="PGVVRWK"/>
    <dbReference type="OrthoDB" id="1200451at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03225">
        <property type="entry name" value="ABC_cobalt_CbiO_domain1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015856">
        <property type="entry name" value="ABC_transpr_CbiO/EcfA_su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="1" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-1278">Translocase</keyword>
    <feature type="domain" description="ABC transporter" evidence="8">
        <location>
            <begin position="10"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="42"/>
            <end position="49"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ABF09792.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="229" mass="24961" checksum="F97329284C6574DF" modified="2006-05-30" version="1">MRTRTPAPLLTVRGLSRRVGARKLFDIAELDFPRATAIALTGVNGAGKSTLLRIVAGLEPAPGATASWTDADGTAHTVPLSPLPMSLRRRIAYMHQHPYLFRTSVRENIAYGLRHHGLPRDEIDRRVGAALGWAGVVHVQDTAPDKLSGGEIQRVALARAKVLEPDLLLLDEPTSSLDGHAREQVIALIGELAAEGRTVVMVCHDRELINLPGVVRWKLGEGRLDTRHK</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="68" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LJ97</accession>
    <name>Q1LJ97_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Transglut_core2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_2906</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0162 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09779.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517453.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2906"/>
    <dbReference type="EnsemblBacteria" id="ABF09779">
        <property type="protein sequence ID" value="ABF09779"/>
        <property type="gene ID" value="Rmet_2906"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2906"/>
    <dbReference type="eggNOG" id="COG2912">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_063810_1_0_4"/>
    <dbReference type="OMA" id="LWIAAEH"/>
    <dbReference type="OrthoDB" id="1023028at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032698">
        <property type="entry name" value="SirB1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13369">
        <property type="entry name" value="Transglut_core2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transglut_core2" evidence="2">
        <location>
            <begin position="42"/>
            <end position="198"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007100"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13369"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF09779.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="282" mass="31860" checksum="1A6C0ADCF777939A" modified="2006-05-30" version="1">MTSTKVLDYFASLVADDDAIPLTETALAIAQDAYPDLDLQAELAALDVLALRLKRRIAEGTAAIQRLRLLNHFFYRDLGFGPNANDYYDPDNSYLNMVLKQRRGIPISLAILYMELGQQIGLPLKGVAFPSHFLVRMTIPAGEVVLDPLTGETLSKEQLQEMLDPYLEREGITNSSEVPLGLFLQTASHREIIARMLRNLKAIYLQESRWQRLLAVQNRLVILLPDSIEEVRDRGLAYANLECFRPALEDLEAYVQARPDAADSTQIRDRMPALRMMSRSLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LJI2</accession>
    <name>Q1LJI2_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">2-hydroxy-3-oxopropionate reductase (Tartronate semialdehyde reductase)</fullName>
            <ecNumber evidence="6">1.1.1.60</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">garR</name>
        <name evidence="6" type="ordered locus">Rmet_2821</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EC" id="1.1.1.60" evidence="6"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09694.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517387.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2821"/>
    <dbReference type="EnsemblBacteria" id="ABF09694">
        <property type="protein sequence ID" value="ABF09694"/>
        <property type="gene ID" value="Rmet_2821"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2821"/>
    <dbReference type="eggNOG" id="COG2084">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035117_1_0_4"/>
    <dbReference type="OMA" id="AWVQSTT"/>
    <dbReference type="OrthoDB" id="1194694at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008679">
        <property type="term" value="F:2-hydroxy-3-oxopropionate reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016054">
        <property type="term" value="P:organic acid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1040.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002204">
        <property type="entry name" value="3-OH-isobutyrate_DH-rel_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008927">
        <property type="entry name" value="6-PGluconate_DH-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013328">
        <property type="entry name" value="6PGD_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006115">
        <property type="entry name" value="6PGDH_NADP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015815">
        <property type="entry name" value="HIBADH-related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029154">
        <property type="entry name" value="NADP-bd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14833">
        <property type="entry name" value="NAD_binding_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03446">
        <property type="entry name" value="NAD_binding_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000103">
        <property type="entry name" value="HIBADH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48179">
        <property type="entry name" value="SSF48179"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00895">
        <property type="entry name" value="3_HYDROXYISOBUT_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0520">NAD</keyword>
    <keyword evidence="1 6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAD_binding_2" evidence="4">
        <location>
            <begin position="4"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="domain" description="NAD_binding_11" evidence="5">
        <location>
            <begin position="165"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="active site" evidence="3">
        <location>
            <position position="171"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR000103-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF03446"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF14833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABF09694.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="289" mass="29794" checksum="7AD74A12A1DED7E4" modified="2006-05-30" version="1">MTTVAFIGLGAMGAPMVGHLIRAGHTVRAFVRRPEAADAARQLGAEPFFTPAEAAQGAAVVFTNVTSSEDVREVLLGRNGVVEGAAPGTICVDHSTISPIVTREIAQALAACGIEALDCPVSGGTVGAQAATLTIMVGGKAEVLEKVRPLLEQIGKTITHIGDSGAGQVAKLCNQIAQVVNIEGIAEAMRFAGAQGVDTAKVFQAMSTGMAGSRMLDMLGPKMVTRDFSAGIESRLHAKDFGLAADIATEIGLELPAMRATSAQLGELMDQGWGKDDTSSLLKVLELKG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2007-10-02" modified="2022-08-03" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LJM9</accession>
    <name>PANC_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Pantothenate synthetase</fullName>
            <shortName evidence="1">PS</shortName>
            <ecNumber evidence="1">6.3.2.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Pantoate--beta-alanine ligase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Pantoate-activating enzyme</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">panC</name>
        <name type="ordered locus">Rmet_2774</name>
    </gene>
    <organism>
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the condensation of pantoate with beta-alanine in an ATP-dependent reaction via a pantoyl-adenylate intermediate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(R)-pantoate + ATP + beta-alanine = (R)-pantothenate + AMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10912"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15980"/>
            <dbReference type="ChEBI" id="CHEBI:29032"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57966"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.3.2.1"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; (R)-pantothenate biosynthesis; (R)-pantothenate from (R)-pantoate and beta-alanine: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="miscellaneous">
        <text evidence="1">The reaction proceeds by a bi uni uni bi ping pong mechanism.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the pantothenate synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.3.2.1" evidence="1"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09647.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517345.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q1LJM9"/>
    <dbReference type="SMR" id="Q1LJM9"/>
    <dbReference type="STRING" id="266264.Rmet_2774"/>
    <dbReference type="EnsemblBacteria" id="ABF09647">
        <property type="protein sequence ID" value="ABF09647"/>
        <property type="gene ID" value="Rmet_2774"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2774"/>
    <dbReference type="eggNOG" id="COG0414">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047148_0_0_4"/>
    <dbReference type="OMA" id="CNHKLEP"/>
    <dbReference type="OrthoDB" id="1661843at2"/>
    <dbReference type="UniPathway" id="UPA00028">
        <property type="reaction ID" value="UER00005"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004592">
        <property type="term" value="F:pantoate-beta-alanine ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015940">
        <property type="term" value="P:pantothenate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00560">
        <property type="entry name" value="PanC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1300.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00158">
        <property type="entry name" value="PanC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004821">
        <property type="entry name" value="Cyt_trans-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003721">
        <property type="entry name" value="Pantoate_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042176">
        <property type="entry name" value="Pantoate_ligase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21299:SF1">
        <property type="entry name" value="PTHR21299:SF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02569">
        <property type="entry name" value="Pantoate_ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00125">
        <property type="entry name" value="cyt_tran_rel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00018">
        <property type="entry name" value="panC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0566">Pantothenate biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000305524" description="Pantothenate synthetase">
        <location>
            <begin position="1"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="1">
        <location>
            <position position="33"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="26"/>
            <end position="33"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="57"/>
        </location>
        <ligand>
            <name>(R)-pantoate</name>
            <dbReference type="ChEBI" id="CHEBI:15980"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="57"/>
        </location>
        <ligand>
            <name>beta-alanine</name>
            <dbReference type="ChEBI" id="CHEBI:57966"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="144"/>
            <end position="147"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="150"/>
        </location>
        <ligand>
            <name>(R)-pantoate</name>
            <dbReference type="ChEBI" id="CHEBI:15980"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="173"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="181"/>
            <end position="184"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00158"/>
        </source>
    </evidence>
    <sequence length="280" mass="31387" checksum="0381A0A2D54292FD" modified="2006-05-30" version="1">MKVISSIQALRDQLRGQNRVAFVPTMGNLHEGHLSLMRLARQHGDPVVASIFVNRLQFGPNEDFDKYPRTLQDDIEKLQSEGVYVLFAPTESDMYPVPQEYRVDPPHDLGDILEGEFRPGFFKGVCTVVMKLFCCVQPRVAVFGKKDYQQLMIVRRMAKQFALPVDIIPAETIRAEDGLALSSRNRYLGETERAEAPVLYKTLHEVRDTVLGGDGVDLLAVEADALARLQARGWKPDYVSIRKQDNLQPPTREEFAAGAPLVVLTAAKLGATRLIDNLEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="113" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LJQ4</accession>
    <name>Q1LJQ4_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Porphobilinogen deaminase</fullName>
            <shortName evidence="8">PBG</shortName>
            <ecNumber evidence="8">2.5.1.61</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Hydroxymethylbilane synthase</fullName>
            <shortName evidence="8">HMBS</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">Pre-uroporphyrinogen synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8 11" type="primary">hemC</name>
        <name evidence="11" type="ordered locus">Rmet_2749</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2 8">Tetrapolymerization of the monopyrrole PBG into the hydroxymethylbilane pre-uroporphyrinogen in several discrete steps.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 8">
            <text>H2O + 4 porphobilinogen = hydroxymethylbilane + 4 NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:13185"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:57845"/>
            <dbReference type="ChEBI" id="CHEBI:58126"/>
            <dbReference type="EC" id="2.5.1.61"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>dipyrromethane</name>
            <dbReference type="ChEBI" id="CHEBI:60342"/>
        </cofactor>
        <text evidence="8">Binds 1 dipyrromethane group covalently.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3 8">Porphyrin-containing compound metabolism; protoporphyrin-IX biosynthesis; coproporphyrinogen-III from 5-aminolevulinate: step 2/4.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5 8">Monomer.</text>
    </comment>
    <comment type="miscellaneous">
        <text evidence="8">The porphobilinogen subunits are added to the dipyrromethane group.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 8">Belongs to the HMBS family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.61" evidence="8"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09622.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517322.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2749"/>
    <dbReference type="EnsemblBacteria" id="ABF09622">
        <property type="protein sequence ID" value="ABF09622"/>
        <property type="gene ID" value="Rmet_2749"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2749"/>
    <dbReference type="eggNOG" id="COG0181">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019704_1_0_4"/>
    <dbReference type="OMA" id="LWQANHI"/>
    <dbReference type="OrthoDB" id="1450400at2"/>
    <dbReference type="UniPathway" id="UPA00251">
        <property type="reaction ID" value="UER00319"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004418">
        <property type="term" value="F:hydroxymethylbilane synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018160">
        <property type="term" value="P:peptidyl-pyrromethane cofactor linkage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006782">
        <property type="term" value="P:protoporphyrinogen IX biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00260">
        <property type="entry name" value="Porphobil_deam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000860">
        <property type="entry name" value="HemC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022419">
        <property type="entry name" value="Porphobilin_deaminase_cofac_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022417">
        <property type="entry name" value="Porphobilin_deaminase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022418">
        <property type="entry name" value="Porphobilinogen_deaminase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036803">
        <property type="entry name" value="Porphobilinogen_deaminase_C_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11557">
        <property type="entry name" value="PTHR11557"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01379">
        <property type="entry name" value="Porphobil_deam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03900">
        <property type="entry name" value="Porphobil_deamC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001438">
        <property type="entry name" value="4pyrrol_synth_OHMeBilane_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00151">
        <property type="entry name" value="PORPHBDMNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54782">
        <property type="entry name" value="SSF54782"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00212">
        <property type="entry name" value="hemC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00533">
        <property type="entry name" value="PORPHOBILINOGEN_DEAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8" id="KW-0627">Porphyrin biosynthesis</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 8 11" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Porphobil_deam" evidence="9">
        <location>
            <begin position="22"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="domain" description="Porphobil_deamC" evidence="10">
        <location>
            <begin position="242"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="modified residue" description="S-(dipyrrolylmethanemethyl)cysteine" evidence="8">
        <location>
            <position position="258"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000416"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002869"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004735"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005638"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00260"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF03900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ABF09622.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="334" mass="35032" checksum="D85BC44AE194F595" modified="2006-05-30" version="1">MQAPANPSSSSAAVSSSMPHKLVIASRESRLAMWQAEHVRAALQQYYPSCDVSILGMTTRGDQILDRTLSKVGGKGLFVKELEVAMSEGRADLAVHSLKDVPMELPEGFTLTAVMEREDPRDALVSAQFASLEEMPAGTVVGTSSLRREAALRSRFPHLVIKPLRGNLDTRLGKLDRGEYGAIILAAAGLKRLGLGDRIRALIAPESSLPAAGQGALGIEIRADRPELAEWLAPLNHLPTLLAVSAERAVSRRLGGSCQVPLAAHARWRDASLQLDAFVALPDGSRQVRAHATGPVATVADAEALGTAVAGDLLAQGADGVLAALSTDSAAGPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LJQ6</accession>
    <name>Q1LJQ6_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Uncharacterized enzyme of heme biosynthesis</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">hemY</name>
        <name evidence="13" type="ordered locus">Rmet_2747</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in a late step of protoheme IX synthesis.</text>
    </comment>
    <comment type="pathway">
        <text evidence="4">Porphyrin-containing compound metabolism; protoheme biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell inner membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09620.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517320.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2747"/>
    <dbReference type="EnsemblBacteria" id="ABF09620">
        <property type="protein sequence ID" value="ABF09620"/>
        <property type="gene ID" value="Rmet_2747"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2747"/>
    <dbReference type="eggNOG" id="COG3071">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037501_0_1_4"/>
    <dbReference type="OMA" id="RASAWRH"/>
    <dbReference type="OrthoDB" id="699592at2"/>
    <dbReference type="UniPathway" id="UPA00252"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042168">
        <property type="term" value="P:heme metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006779">
        <property type="term" value="P:porphyrin-containing compound biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005254">
        <property type="entry name" value="Heme_biosyn_assoc_TPR_pro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010817">
        <property type="entry name" value="HemY_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07219">
        <property type="entry name" value="HemY_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="SSF48452"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00540">
        <property type="entry name" value="TPR_hemY_coli"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0627">Porphyrin biosynthesis</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="40"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="domain" description="HemY_N" evidence="12">
        <location>
            <begin position="27"/>
            <end position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004429"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004744"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF07219"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ABF09620.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="396" mass="45448" checksum="BC77DC3F196123C5" modified="2006-05-30" version="1">MRLLFWVAVLFGGAVGLALFTQFNQSNVVLFYPPYRVEMSLNLTLALLLLAFFIVWTVLSTFRHLSEMPKRAAAYRERSRMSKAQAAMRESIENLFAGRFARAERAAHEAQAWPEQAQTAALVGARAAHRMQESERRDAWMAQVTDPEREQARLVSMAELLVDARDAEGALATIAQLQEKGGRQIHVQRIALRAHQHLKNWSEVLRLARSLEKRNALHPVLALRLKQMAVEAMLEERRHDAEALFHFWRSLTADERHAVRIAELGARYFSSLDKQNEARRIVEEALKVNWDSRLVQRYADCAEPGKALPQIQQAEKWLVDHPTDADLYYTLGVLCLGEQLWGKAQSNFERTLKYADRDAQRRICIRAHLALARLFEETDRAEDAQRHYREGAMLAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LJR2</accession>
    <name>Q1LJR2_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Bifunctional protein: pyrimidine operon regulatory protein/uracil phosphoribosyltransferase (UPRTase)</fullName>
            <ecNumber evidence="2">2.4.2.9</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">pyrR</name>
        <name evidence="2" type="ordered locus">Rmet_2741</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.4.2.9" evidence="2"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09614.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517314.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2741"/>
    <dbReference type="EnsemblBacteria" id="ABF09614">
        <property type="protein sequence ID" value="ABF09614"/>
        <property type="gene ID" value="Rmet_2741"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2741"/>
    <dbReference type="eggNOG" id="COG2065">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_094234_1_1_4"/>
    <dbReference type="OMA" id="PVSANYV"/>
    <dbReference type="OrthoDB" id="1725260at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004845">
        <property type="term" value="F:uracil phosphoribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd06223">
        <property type="entry name" value="PRTases_typeI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2020">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000836">
        <property type="entry name" value="PRibTrfase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029057">
        <property type="entry name" value="PRTase-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00156">
        <property type="entry name" value="Pribosyltran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53271">
        <property type="entry name" value="SSF53271"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Pribosyltran" evidence="1">
        <location>
            <begin position="27"/>
            <end position="138"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF09614.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="174" mass="18820" checksum="196BA584D2B47715" modified="2006-05-30" version="1">MTQTSTPDAEALYHALRDQAQALMPSSERARWSVAGIYSGGAWIAERLASDLQLGGHGVINVAFHRDDYAKKGLHSQAQSTTLPFEVEDRNILLIDDVLATGRTIRAAVNELFDYGRPARVALAVLADRGGRELPIAAEFAAVTVALPQDKTLVLSRQGEGADTRFAFATEVRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LJV7</accession>
    <name>Q1LJV7_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Aminotransferase</fullName>
            <ecNumber evidence="1">2.6.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_2696</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.6.1.-" evidence="1"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09569.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517268.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2696"/>
    <dbReference type="EnsemblBacteria" id="ABF09569">
        <property type="protein sequence ID" value="ABF09569"/>
        <property type="gene ID" value="Rmet_2696"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2696"/>
    <dbReference type="eggNOG" id="COG0436">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017584_4_3_4"/>
    <dbReference type="OMA" id="IHMEVGQ"/>
    <dbReference type="OrthoDB" id="554560at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008483">
        <property type="term" value="F:transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901605">
        <property type="term" value="P:alpha-amino acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009058">
        <property type="term" value="P:biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004838">
        <property type="entry name" value="NHTrfase_class1_PyrdxlP-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="SSF53383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00105">
        <property type="entry name" value="AA_TRANSFER_CLASS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 3" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aminotran_1_2" evidence="2">
        <location>
            <begin position="36"/>
            <end position="388"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU000481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF09569.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="393" mass="42745" checksum="A44FCAEAD0E60255" modified="2006-05-30" version="1">MDLQRLATASRLANIDAFHVMELAKQAAELERAGRHIIHMGIGEPDFTAAAPVIHAAEAAMRRGVTQYTGALGIHALREAIAGYYKTSYGIDVPARRVIVTAGASGALLLACAVLVEIGAEVLMPDPSYPCNRHFVAAFDGHAKMIPSGPEERFQLTAAQVIEHWGENTRGVLLASPSNPTGTSILPDELKAILAEVRKRQGFAILDEIYQGLSYDAPPVSALSLDDNVVTVNSFSKYFNMTGWRLGWLVVPDSLVPAFEKVAQNLFICASAVAQHAALACFTPEALAIYDERKAEFRRRRDAIVPALEALGLRVPVKPDGAFYVYANCRHVNHPAAGNADRLTQAILHDAGVVMVPGTDFGPHTANDYIRISYATSMQNIEEAMQRLHHFLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LK44</accession>
    <name>Q1LK44_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">2-nitropropane dioxygenase</fullName>
            <ecNumber evidence="2">1.13.12.16</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_2605</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EC" id="1.13.12.16" evidence="2"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09482.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517185.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2605"/>
    <dbReference type="EnsemblBacteria" id="ABF09482">
        <property type="protein sequence ID" value="ABF09482"/>
        <property type="gene ID" value="Rmet_2605"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2605"/>
    <dbReference type="eggNOG" id="COG2070">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038732_1_0_4"/>
    <dbReference type="OMA" id="VMGSRFM"/>
    <dbReference type="OrthoDB" id="275153at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051213">
        <property type="term" value="F:dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018580">
        <property type="term" value="F:nitronate monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd04730">
        <property type="entry name" value="NPD_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004136">
        <property type="entry name" value="NMO"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03060">
        <property type="entry name" value="NMO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NMO" evidence="1">
        <location>
            <begin position="16"/>
            <end position="324"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03060"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF09482.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="342" mass="35868" checksum="63A55430035E6A0F" modified="2006-05-30" version="1">MSPAASGHAAERIFQTRLAALLDIRYPILLGGMQLLGTSDVVAAVVNAGAMGFITARSFPTLEAFRADLRRCRTLTRGRPFGVNVTLSAREDRNQLAAAWIDVALDEGVRVFESAGMSPERLVAPIHAGGGLLIHKCPSVRHAVTAQRLGVDAVALVGLEEGGHPGANQLSAFVNGAFALERVEIPLVIGGGIGSGRQIAAALALGADGVVMGSRFMVAAEIATHRALKERIVAADEHCSIAILRSLNDTWRVLDNAAAREVQRLEAEGARSHAAFGDLILSSRTQERVYLGGDTDAGIVSLGPAGGFADAIESAGKIVERLVEGACAAWEVMTGRVERAWA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LK80</accession>
    <name>Q1LK80_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 7">Dihydrofolate reductase</fullName>
            <ecNumber evidence="3 7">1.5.1.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">folA</name>
        <name evidence="10" type="ordered locus">Rmet_2569</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6 7">Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>(6S)-5,6,7,8-tetrahydrofolate + NADP(+) = 7,8-dihydrofolate + H(+) + NADPH</text>
            <dbReference type="Rhea" id="RHEA:15009"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57451"/>
            <dbReference type="ChEBI" id="CHEBI:57453"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.5.1.3"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 7">Cofactor biosynthesis; tetrahydrofolate biosynthesis; 5,6,7,8-tetrahydrofolate from 7,8-dihydrofolate: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 7 8">Belongs to the dihydrofolate reductase family.</text>
    </comment>
    <dbReference type="EC" id="1.5.1.3" evidence="3 7"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09446.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517149.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2569"/>
    <dbReference type="EnsemblBacteria" id="ABF09446">
        <property type="protein sequence ID" value="ABF09446"/>
        <property type="gene ID" value="Rmet_2569"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2569"/>
    <dbReference type="eggNOG" id="COG0262">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_043966_5_1_4"/>
    <dbReference type="OMA" id="RDNQLPW"/>
    <dbReference type="OrthoDB" id="1920912at2"/>
    <dbReference type="UniPathway" id="UPA00077">
        <property type="reaction ID" value="UER00158"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004146">
        <property type="term" value="F:dihydrofolate reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006545">
        <property type="term" value="P:glycine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006730">
        <property type="term" value="P:one-carbon metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046654">
        <property type="term" value="P:tetrahydrofolate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00209">
        <property type="entry name" value="DHFR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.430.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012259">
        <property type="entry name" value="DHFR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024072">
        <property type="entry name" value="DHFR-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017925">
        <property type="entry name" value="DHFR_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001796">
        <property type="entry name" value="DHFR_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48069">
        <property type="entry name" value="PTHR48069"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00186">
        <property type="entry name" value="DHFR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000194">
        <property type="entry name" value="DHFR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53597">
        <property type="entry name" value="SSF53597"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00075">
        <property type="entry name" value="DHFR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51330">
        <property type="entry name" value="DHFR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0521">NADP</keyword>
    <keyword evidence="7" id="KW-0554">One-carbon metabolism</keyword>
    <keyword evidence="5 7 10" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DHFR" evidence="9">
        <location>
            <begin position="3"/>
            <end position="160"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004903"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009539"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012856"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00025067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR000194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU004474"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ABF09446.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="166" mass="18486" checksum="413E77B32C9DBD81" modified="2006-05-30" version="1">MTLLTLIVARARNGVIGRNNTLPWRLPEDLQHFKRTTMGAPIIMGRKTWDSIGRPLPGRRNIVVTRNRDLHIEGADVVGSIEDAQRLCIGAEQVFLIGGAQLYAEALPSADRLIVTEIDADVEGDAFFPAVDRTTWVETARELHHSETNGFDYAFVTYERPASGEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="52" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LKA3</accession>
    <name>Q1LKA3_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_2546</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09423.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517123.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF09423">
        <property type="protein sequence ID" value="ABF09423"/>
        <property type="gene ID" value="Rmet_2546"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2546"/>
    <dbReference type="eggNOG" id="ENOG5033A39">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_171090_0_0_4"/>
    <dbReference type="OMA" id="PAKRYGW"/>
    <dbReference type="OrthoDB" id="1836617at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="24"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="51"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF09423.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="82" mass="9770" checksum="0473570B9FE6CD39" modified="2006-05-30" version="1">MQTDPEYWFHAKRYGWGWGLPTCWQGWIAYGAYFALLALAVFFFRVDHRPVVFVFLVVILSLALFVVCWIKGEPPRWRWGDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="67" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LKE4</accession>
    <name>Q1LKE4_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">G:T/U mismatch-specific DNA glycosylase-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_2505</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09382.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517083.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2505"/>
    <dbReference type="EnsemblBacteria" id="ABF09382">
        <property type="protein sequence ID" value="ABF09382"/>
        <property type="gene ID" value="Rmet_2505"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2505"/>
    <dbReference type="eggNOG" id="COG3663">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_094865_0_1_4"/>
    <dbReference type="OMA" id="PETNDFA"/>
    <dbReference type="OrthoDB" id="2034230at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.470.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026353">
        <property type="entry name" value="Hypoxan-DNA_Glyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005122">
        <property type="entry name" value="Uracil-DNA_glycosylase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036895">
        <property type="entry name" value="Uracil-DNA_glycosylase-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03167">
        <property type="entry name" value="UDG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00986">
        <property type="entry name" value="UDG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52141">
        <property type="entry name" value="SSF52141"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR04274">
        <property type="entry name" value="hypoxanDNAglyco"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UDG" evidence="1">
        <location>
            <begin position="8"/>
            <end position="160"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00986"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF09382.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="164" mass="18270" checksum="32B5C546B041B5B3" modified="2006-05-30" version="1">MTTKRCFPPVVDIHTRVLVLGSLPGEVSLAQSRYYAHKQNRFWHLMSDVLGVDLVGLEYEARLQALLDHRVGLWDVVAEARREGSLDSSIRDHQGNDLIGLIATLPTLKAIAFNGGTAAKIGEKTLADHGDRHAILRLPSSSPAYTLAYAEKRKAWESLRDWLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LKL4</accession>
    <name>Q1LKL4_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Predicted methyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_2435</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09312.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517029.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2435"/>
    <dbReference type="EnsemblBacteria" id="ABF09312">
        <property type="protein sequence ID" value="ABF09312"/>
        <property type="gene ID" value="Rmet_2435"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2435"/>
    <dbReference type="eggNOG" id="COG0313">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_044779_4_1_4"/>
    <dbReference type="OMA" id="MSEAGCP"/>
    <dbReference type="OrthoDB" id="1059309at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.950.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1010.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035996">
        <property type="entry name" value="4pyrrol_Methylase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014777">
        <property type="entry name" value="4pyrrole_Mease_sub1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014776">
        <property type="entry name" value="4pyrrole_Mease_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008189">
        <property type="entry name" value="rRNA_ssu_MeTfrase_I"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46111">
        <property type="entry name" value="PTHR46111"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005917">
        <property type="entry name" value="MTase_YraL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53790">
        <property type="entry name" value="SSF53790"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF09312.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="243" mass="26346" checksum="539A515D254212E6" modified="2006-05-30" version="1">MAGNLFLIPNTLGKRDEFDPVMDVIPAGVQQIAARLDYLVAENAKTARAFLKKLSETTPLAQPIQQIEIRELNVNTRPDTLTALLDPIAAGRDGGLLSEAGVPAVADPGADLVRLAHTRGITVRPLVGPSSILLAVMGSGLNGQSFAFNGYLPVDADVRAQKLRELEQRSRKSHQTQVWIETPYRNGALLEALRQHCSGTTLLSIAVDLTLPTEAIVTLPISAWRPDRLDLHKRPAIFSLLAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-10-07" version="68" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LKN0</accession>
    <name>Q1LKN0_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_2419</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09296.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2419"/>
    <dbReference type="EnsemblBacteria" id="ABF09296">
        <property type="protein sequence ID" value="ABF09296"/>
        <property type="gene ID" value="Rmet_2419"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2419"/>
    <dbReference type="eggNOG" id="COG4969">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_149778_0_0_4"/>
    <dbReference type="OMA" id="FSYSREI"/>
    <dbReference type="OrthoDB" id="2013880at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032314">
        <property type="entry name" value="DUF4845"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16137">
        <property type="entry name" value="DUF4845"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="21"/>
            <end position="43"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF09296.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="130" mass="14314" checksum="4FE8E2E3D28DC5B3" modified="2006-05-30" version="1">MGQKRLGSQGRVRFDVIHRARGFSLGSLLVVFIIVGAVAVPALKVIPSLIEYFSIKRAVNYAKGTGTKRSEVAASFDKQATIDRISSIKGEDLDIEEDETGRIRAVGFEYRTEVQIYGPLSFLITYSGTQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="112" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LKR9</accession>
    <name>Q1LKR9_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Apolipoprotein N-acyltransferase</fullName>
            <shortName evidence="9">ALP N-acyltransferase</shortName>
            <ecNumber evidence="9">2.3.1.269</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">cutE</name>
        <name evidence="9" type="synonym">lnt</name>
        <name evidence="12" type="ordered locus">Rmet_2380</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Catalyzes the phospholipid dependent N-acylation of the N-terminal cysteine of apolipoprotein, the last step in lipoprotein maturation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>a glycerophospholipid + N-terminal S-1,2-diacyl-sn-glyceryl-L-cysteinyl-[lipoprotein] = a 2-acyl-sn-glycero-3-phospholipid + H(+) + N-acyl-S-1,2-diacyl-sn-glyceryl-L-cysteinyl-[lipoprotein]</text>
            <dbReference type="Rhea" id="RHEA:48228"/>
            <dbReference type="Rhea" id="RHEA-COMP:14681"/>
            <dbReference type="Rhea" id="RHEA-COMP:14684"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:136912"/>
            <dbReference type="ChEBI" id="CHEBI:140656"/>
            <dbReference type="ChEBI" id="CHEBI:140657"/>
            <dbReference type="ChEBI" id="CHEBI:140660"/>
            <dbReference type="EC" id="2.3.1.269"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="9">Protein modification; lipoprotein biosynthesis (N-acyl transfer).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cell inner membrane</location>
            <topology evidence="9">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the CN hydrolase family. Apolipoprotein N-acyltransferase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.269" evidence="9"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09257.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_003149811.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2380"/>
    <dbReference type="EnsemblBacteria" id="ABF09257">
        <property type="protein sequence ID" value="ABF09257"/>
        <property type="gene ID" value="Rmet_2380"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2380"/>
    <dbReference type="eggNOG" id="COG0815">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019563_3_0_4"/>
    <dbReference type="OMA" id="YVALVPW"/>
    <dbReference type="OrthoDB" id="1291198at2"/>
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        <property type="project" value="UniProtKB-KW"/>
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    </dbReference>
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        <property type="term" value="P:lipoprotein biosynthetic process"/>
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        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="Apolipo_AcylTrfase"/>
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        <property type="entry name" value="C-N_Hydrolase"/>
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        <property type="entry name" value="C-N_Hydrolase_sf"/>
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    <dbReference type="InterPro" id="IPR045378">
        <property type="entry name" value="LNT_N"/>
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        <property type="entry name" value="CN_hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 9 12" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="9" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="3 9" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="12" id="KW-0449">Lipoprotein</keyword>
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    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0732">Signal</keyword>
    <keyword evidence="4 9 12" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5005693586" description="Apolipoprotein N-acyltransferase" evidence="10">
        <location>
            <begin position="22"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="62"/>
            <end position="79"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="91"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="125"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="165"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="203"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="domain" description="CN hydrolase" evidence="11">
        <location>
            <begin position="237"/>
            <end position="499"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00010065"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
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    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
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    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
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    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
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    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
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    </evidence>
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        <source>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50263"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="ABF09257.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="532" mass="57343" checksum="DB0392767B256E2A" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="69" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LKT5</accession>
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        <submittedName>
            <fullName evidence="4">Relaxase</fullName>
        </submittedName>
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        <name evidence="4" type="ordered locus">Rmet_2364</name>
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        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
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            <taxon>Bacteria</taxon>
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            <taxon>Betaproteobacteria</taxon>
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            <taxon>Burkholderiaceae</taxon>
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        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="ABF09241.1"/>
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        <property type="component" value="Chromosome"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="ICE_relaxase_PFGI-1"/>
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        <property type="entry name" value="TraI_2_C"/>
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        <property type="entry name" value="Unchr_helicase_relaxase_TraI"/>
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        <property type="entry name" value="WH-like_DNA-bd_sf"/>
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    </dbReference>
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    </dbReference>
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            <end position="389"/>
        </location>
    </feature>
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    </feature>
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        <location>
            <begin position="494"/>
            <end position="540"/>
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    </feature>
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        <location>
            <begin position="17"/>
            <end position="32"/>
        </location>
    </feature>
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        <location>
            <begin position="494"/>
            <end position="510"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="526"/>
            <end position="540"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF07515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF09241.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="680" mass="74534" checksum="FBFF6A553F9236FC" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-02-05" modified="2022-08-03" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LL27</accession>
    <name>ACSA_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Acetyl-coenzyme A synthetase</fullName>
            <shortName evidence="1">AcCoA synthetase</shortName>
            <shortName evidence="1">Acs</shortName>
            <ecNumber evidence="1">6.2.1.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Acetate--CoA ligase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Acyl-activating enzyme</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">acsA</name>
        <name type="ordered locus">Rmet_2270</name>
    </gene>
    <organism>
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the conversion of acetate into acetyl-CoA (AcCoA), an essential intermediate at the junction of anabolic and catabolic pathways. AcsA undergoes a two-step reaction. In the first half reaction, AcsA combines acetate with ATP to form acetyl-adenylate (AcAMP) intermediate. In the second half reaction, it can then transfer the acetyl group from AcAMP to the sulfhydryl group of CoA, forming the product AcCoA.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>acetate + ATP + CoA = acetyl-CoA + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:23176"/>
            <dbReference type="ChEBI" id="CHEBI:30089"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.2.1.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="PTM">
        <text evidence="1">Acetylated. Deacetylation by the SIR2-homolog deacetylase activates the enzyme.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the ATP-dependent AMP-binding enzyme family.</text>
    </comment>
    <dbReference type="EC" id="6.2.1.1" evidence="1"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09149.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516974.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q1LL27"/>
    <dbReference type="SMR" id="Q1LL27"/>
    <dbReference type="STRING" id="266264.Rmet_2270"/>
    <dbReference type="EnsemblBacteria" id="ABF09149">
        <property type="protein sequence ID" value="ABF09149"/>
        <property type="gene ID" value="Rmet_2270"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2270"/>
    <dbReference type="eggNOG" id="COG0365">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000022_3_6_4"/>
    <dbReference type="OMA" id="DHWWHDL"/>
    <dbReference type="OrthoDB" id="141801at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003987">
        <property type="term" value="F:acetate-CoA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016208">
        <property type="term" value="F:AMP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019427">
        <property type="term" value="P:acetyl-CoA biosynthetic process from acetate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01123">
        <property type="entry name" value="Ac_CoA_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Ac_CoA_lig"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032387">
        <property type="entry name" value="ACAS_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025110">
        <property type="entry name" value="AMP-bd_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045851">
        <property type="entry name" value="AMP-bd_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020845">
        <property type="entry name" value="AMP-binding_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_Synth/Lig"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16177">
        <property type="entry name" value="ACAS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13193">
        <property type="entry name" value="AMP-binding_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02188">
        <property type="entry name" value="Ac_CoA_lig_AcsA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00455">
        <property type="entry name" value="AMP_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0007">Acetylation</keyword>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_1000065309" description="Acetyl-coenzyme A synthetase">
        <location>
            <begin position="1"/>
            <end position="660"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="197"/>
            <end position="200"/>
        </location>
        <ligand>
            <name>CoA</name>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="317"/>
        </location>
        <ligand>
            <name>CoA</name>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="397"/>
            <end position="399"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="421"/>
            <end position="426"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="512"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="528"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="536"/>
        </location>
        <ligand>
            <name>CoA</name>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="539"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="550"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="555"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="modified residue" description="N6-acetyllysine" evidence="1">
        <location>
            <position position="625"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01123"/>
        </source>
    </evidence>
    <sequence length="660" mass="72399" checksum="D4A0F2E570A400B8" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LL60</accession>
    <name>Q1LL60_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Nucleoside diphosphate hydrolase protein (NUDIX)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_2237</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09116.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516942.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2237"/>
    <dbReference type="EnsemblBacteria" id="ABF09116">
        <property type="protein sequence ID" value="ABF09116"/>
        <property type="gene ID" value="Rmet_2237"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2237"/>
    <dbReference type="eggNOG" id="COG0494">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_048013_1_1_4"/>
    <dbReference type="OMA" id="LQPECEY"/>
    <dbReference type="OrthoDB" id="1345242at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031804">
        <property type="entry name" value="DUF4743"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015797">
        <property type="entry name" value="NUDIX_hydrolase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000086">
        <property type="entry name" value="NUDIX_hydrolase_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15916">
        <property type="entry name" value="DUF4743"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00293">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55811">
        <property type="entry name" value="SSF55811"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51462">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nudix hydrolase" evidence="1">
        <location>
            <begin position="131"/>
            <end position="272"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF09116.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="283" mass="30345" checksum="56F1351C59835675" modified="2006-05-30" version="1">MAETVSRPADPADIVSVVTAGVAARSPFDPVSHLRLMVDGRHVGWLPRKHADILAGFDGVLGQPDGQGAITLLPGRTTVPARNAALATLATQLADAGHVRGWRNELFAVTPTLEAEPLAVIERAAARFLGLLTFASHMNGIVAGQRALWISRRSPRKAVDPGMWDNLVAGGMPAGSDPLETLVRECDEESGIPPALASQAEAHGVIDVLREIPEGVQWERVYVYDLMLPADFVPHNRDGEVAEHRHIDPEALLAIMADCAMTVDATLVTLDALRRRGWAGIQP</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2007-04-03" modified="2022-08-03" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LLI5</accession>
    <name>RLMD_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD</fullName>
            <ecNumber evidence="1">2.1.1.190</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">23S rRNA(m5U1939)-methyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rlmD</name>
        <name type="synonym">rumA</name>
        <name type="ordered locus">Rmet_2112</name>
    </gene>
    <organism>
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the formation of 5-methyl-uridine at position 1939 (m5U1939) in 23S rRNA.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-adenosyl-L-methionine + uridine(1939) in 23S rRNA = 5-methyluridine(1939) in 23S rRNA + H(+) + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:42908"/>
            <dbReference type="Rhea" id="RHEA-COMP:10278"/>
            <dbReference type="Rhea" id="RHEA-COMP:10279"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="ChEBI" id="CHEBI:74447"/>
            <dbReference type="EC" id="2.1.1.190"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class I-like SAM-binding methyltransferase superfamily. RNA M5U methyltransferase family. RlmD subfamily.</text>
    </comment>
    <comment type="sequence caution" evidence="3">
        <conflict type="erroneous initiation">
            <sequence resource="EMBL-CDS" id="ABF08991" version="1"/>
        </conflict>
    </comment>
    <dbReference type="EC" id="2.1.1.190" evidence="1"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08991.1"/>
        <property type="status" value="ALT_INIT"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q1LLI5"/>
    <dbReference type="SMR" id="Q1LLI5"/>
    <dbReference type="STRING" id="266264.Rmet_2112"/>
    <dbReference type="EnsemblBacteria" id="ABF08991">
        <property type="protein sequence ID" value="ABF08991"/>
        <property type="gene ID" value="Rmet_2112"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2112"/>
    <dbReference type="eggNOG" id="COG2265">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014689_8_2_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070041">
        <property type="term" value="F:rRNA (uridine-C5-)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01010">
        <property type="entry name" value="23SrRNA_methyltr_RlmD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001566">
        <property type="entry name" value="23S_rRNA_MeTrfase_RlmD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030391">
        <property type="entry name" value="MeTrfase_TrmA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002792">
        <property type="entry name" value="TRAM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010280">
        <property type="entry name" value="U5_MeTrfase_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11061">
        <property type="entry name" value="PTHR11061"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05958">
        <property type="entry name" value="tRNA_U5-meth_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51687">
        <property type="entry name" value="SAM_MT_RNA_M5U"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50926">
        <property type="entry name" value="TRAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01231">
        <property type="entry name" value="TRMA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0004">4Fe-4S</keyword>
    <keyword id="KW-0408">Iron</keyword>
    <keyword id="KW-0411">Iron-sulfur</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0489">Methyltransferase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0698">rRNA processing</keyword>
    <keyword id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000282058" description="23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD">
        <location>
            <begin position="1"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="domain" description="TRAM" evidence="1">
        <location>
            <begin position="17"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="1">
        <location>
            <position position="422"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="94"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="100"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="103"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="182"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="290"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="319"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="324"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="340"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="368"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="389"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="466" mass="51058" checksum="5892826115D51817" modified="2007-04-03" version="2">MSSQPNPTSHPEAASAASAASNDPVVKIDSLDMEARGVGRLENEDGTPGKVIFVEGALPGETVAYRSYRRKPSYEQAHLVEVKQASVMRVKPGCQHFGVCGGCSMQHLDSRAQLAIKQRVLEDNLWHLSKVKPDVVFRPIAGPDWGYRYRARLTVRHVVKKGGVLVGFHERKSSYVADMTHCEILPPHVSAMLVPLRELVGGLSIHDRMPQIELAVGAEVTALVLRVLEPLNDADKDLLRAFADQHQVQFWLQPKGPDTVYPFYPAEQELAYTLPEFGIRMPFKPTDFTQVNHQINRVLIGRALRLLDAKPTDRLLDLFCGIGNFTLPLATQGASVMGIEGSEVLTTRALANAEYNGLAAKTSFACRNLFEVTAEDIAALGRFDRWLVDPPREGALAVCKALAELSQAGSDVLPKRIVYVSCSPATLARDAGLLVHEAGYRLAGAGVVNMFPHTSHVESIAVFERD</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LLJ3</accession>
    <name>Q1LLJ3_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Ancillary SecYEG translocon subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Rmet_2104</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the YfgM family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08983.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516817.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2104"/>
    <dbReference type="EnsemblBacteria" id="ABF08983">
        <property type="protein sequence ID" value="ABF08983"/>
        <property type="gene ID" value="Rmet_2104"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2104"/>
    <dbReference type="eggNOG" id="COG2976">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_084785_1_1_4"/>
    <dbReference type="OMA" id="RFFANNG"/>
    <dbReference type="OrthoDB" id="1926596at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044877">
        <property type="term" value="F:protein-containing complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018704">
        <property type="entry name" value="TPR_21"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026039">
        <property type="entry name" value="YfgM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38035">
        <property type="entry name" value="PTHR38035"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09976">
        <property type="entry name" value="TPR_21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006170">
        <property type="entry name" value="YfgM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="SSF48452"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0143">Chaperone</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="24"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="domain" description="TPR_21" evidence="5">
        <location>
            <begin position="15"/>
            <end position="208"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00024197"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00024235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF09976"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABF08983.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="210" mass="23142" checksum="72CAD511BF3D29E5" modified="2006-05-30" version="1">MAYDLEEQEQLESLKAWWKQYGNAVTWALIVILLGLAAWSGWGYWQRVQARDASLLYEQVTKAAESRDADRVKRAATDLEEKFGRTAYGPMSALVAAKVLYEAGDLAGAKTQLQWAIDHGGDDYAPLARVRMAGVLLDEKAYDQGLALLKDEPAAPYAALFADRRGDLLAAQDKREEARTAYRSALDKLGSGEAAMRQIIQFKLDALGAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LLM9</accession>
    <name>Q1LLM9_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_2068</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08947.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516781.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2068"/>
    <dbReference type="EnsemblBacteria" id="ABF08947">
        <property type="protein sequence ID" value="ABF08947"/>
        <property type="gene ID" value="Rmet_2068"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2068"/>
    <dbReference type="eggNOG" id="COG3391">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009318_2_2_4"/>
    <dbReference type="OMA" id="KTLWVNN"/>
    <dbReference type="OrthoDB" id="302683at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019405">
        <property type="entry name" value="Lactonase_7-beta_prop"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011045">
        <property type="entry name" value="N2O_reductase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10282">
        <property type="entry name" value="Lactonase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50974">
        <property type="entry name" value="SSF50974"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193198" evidence="1">
        <location>
            <begin position="29"/>
            <end position="328"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF08947.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="328" mass="35848" checksum="8666ADCD273F9A2A" modified="2006-05-30" version="1">MLALRRVVAGAVSVGALALALVSAPARAEVVVILNSGDASVTLIDKDTQKVLETFPIGKEPHHLIATPDDKSLIVANAVGNDLVFLDPVTGKVQQRVPNIDDPYQIGFSPDQKWFIATGNRLDRVDVYRWDGRQLKIAKQFPLAKTPSHIAFTADSKIAFITLQDSNQIAAIDLTTLTVLWTMEAGSAPAGIWVTPDQKYLLVGMTGADYVAVIDWRTRTVVKQIQTGKGAHNFRAVGDKRHLFLSNRVSGSISIIDQQTLSKVGEITGLLPGPDDMELTADGKTLWVTFRWARSVGLIDVASRKMIKTIRVGKSPHGIYFRTRAPLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="67" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LLN1</accession>
    <name>Q1LLN1_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">C-5 sterol desaturase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_2066</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08945.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516779.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2066"/>
    <dbReference type="EnsemblBacteria" id="ABF08945">
        <property type="protein sequence ID" value="ABF08945"/>
        <property type="gene ID" value="Rmet_2066"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2066"/>
    <dbReference type="eggNOG" id="COG3000">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_857763_0_0_4"/>
    <dbReference type="OMA" id="WSDNRNH"/>
    <dbReference type="OrthoDB" id="2048011at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008610">
        <property type="term" value="P:lipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006694">
        <property type="entry name" value="Fatty_acid_hydroxylase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04116">
        <property type="entry name" value="FA_hydroxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="47"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="85"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="127"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="domain" description="Fatty acid hydroxylase" evidence="2">
        <location>
            <begin position="140"/>
            <end position="286"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF04116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF08945.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="331" mass="38068" checksum="E1EF711479CD3DD9" modified="2006-05-30" version="1">MWDALTNWLNLWVGQIEATVFQNVVLPVVYNLGFGGYAEDAFTGTEWLLVGLVQIAVMVLILGPLERLRPVEVVTDRAAIRTDILYTLIHRLGLFRLAMFFLLAPLMDGIEGHLRLLGWQRVNVEDWWPGVTSIPLISFSLYLALFDLLDYWYHRLSHTYRWWWSLHAVHHSQRQMTLWSDNRNHLLDDILRDFVFAAVALAVGVEPSQYVLLVALSQLLQSLQHANLRLHFGWLGERLLISPRFHRTHHAVGLGHEKRATAEKPARLGGCNYGVIFPWWDMLFGTADFTDVYHPTGIRDQMPPPAGRARSYGDGFLSQQWLGIKRLLRLG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-02-05" modified="2022-05-25" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LLR4</accession>
    <name>RIMP_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Ribosome maturation factor RimP</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rimP</name>
        <name type="ordered locus">Rmet_2033</name>
    </gene>
    <organism>
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Required for maturation of 30S ribosomal subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the RimP family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08912.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516747.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q1LLR4"/>
    <dbReference type="SMR" id="Q1LLR4"/>
    <dbReference type="STRING" id="266264.Rmet_2033"/>
    <dbReference type="EnsemblBacteria" id="ABF08912">
        <property type="protein sequence ID" value="ABF08912"/>
        <property type="gene ID" value="Rmet_2033"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2033"/>
    <dbReference type="eggNOG" id="COG0779">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_070525_1_0_4"/>
    <dbReference type="OMA" id="YIHVSLY"/>
    <dbReference type="OrthoDB" id="1595498at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042274">
        <property type="term" value="P:ribosomal small subunit biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01734">
        <property type="entry name" value="YlxS_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01077">
        <property type="entry name" value="RimP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003728">
        <property type="entry name" value="Ribosome_maturation_RimP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028998">
        <property type="entry name" value="RimP_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036847">
        <property type="entry name" value="RimP_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028989">
        <property type="entry name" value="RimP_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035956">
        <property type="entry name" value="RimP_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33867">
        <property type="entry name" value="PTHR33867"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02576">
        <property type="entry name" value="DUF150"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17384">
        <property type="entry name" value="DUF150_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF74942">
        <property type="entry name" value="SSF74942"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75420">
        <property type="entry name" value="SSF75420"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0690">Ribosome biogenesis</keyword>
    <feature type="chain" id="PRO_1000064753" description="Ribosome maturation factor RimP">
        <location>
            <begin position="1"/>
            <end position="162"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01077"/>
        </source>
    </evidence>
    <sequence length="162" mass="17857" checksum="F5041DE521855A84" modified="2006-05-30" version="1">MHLADLIETTLIGMGYELVELERAPAGLLRVYIDQPETGIVIEDCEKVSRQLTHVLTVENVDYERLEVSSPGLDRPLKKLADFVRFAGAEVRVTLRLPVNGQKNFVGILCEPAGDAGAEKIGLEFEGKDGPALLEFAISDVDRARLVPVIDFKGNQRKGNKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LLS8</accession>
    <name>Q1LLS8_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_2019</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08898.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516733.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2019"/>
    <dbReference type="EnsemblBacteria" id="ABF08898">
        <property type="protein sequence ID" value="ABF08898"/>
        <property type="gene ID" value="Rmet_2019"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2019"/>
    <dbReference type="HOGENOM" id="CLU_1894491_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF08898.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="134" mass="15184" checksum="7A836A2521AAFCF2" modified="2006-05-30" version="1">MSSIRCQELLRLYDIHISNALARQFAGAIGKAPDTGENVLLMLVQTDELLNKNGAVEADRLDPRWSAWDKRKEAAMFYGGLDEARAKNLPVHVYFGYRTSPNAPTKYVEPPTVSRLALWDGRRYRTEPVRATHP</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LM38</accession>
    <name>Q1LM38_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Zinc-containing alcohol dehydrogenase superfamily</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_1909</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08788.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1909"/>
    <dbReference type="EnsemblBacteria" id="ABF08788">
        <property type="protein sequence ID" value="ABF08788"/>
        <property type="gene ID" value="Rmet_1909"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1909"/>
    <dbReference type="eggNOG" id="COG0604">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026673_3_1_4"/>
    <dbReference type="OMA" id="FYRGQHT"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013149">
        <property type="entry name" value="ADH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020843">
        <property type="entry name" value="PKS_ER"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00107">
        <property type="entry name" value="ADH_zinc_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00829">
        <property type="entry name" value="PKS_ER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="SSF50129"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PKS_ER" evidence="1">
        <location>
            <begin position="15"/>
            <end position="327"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF08788.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="329" mass="34526" checksum="0599F35FD718120D" modified="2006-05-30" version="1">MPIIMNTIQVKRKAANIDELDLTLLGVPRPEAADGECVIEVTSAGVNPSDVKATLGLMPHAVWPRTPGRDYAGVVIDGPAALMGQQVWGSGGELGIRRDGTHGKYLRVNAQSVRAKPATLSLLEAGAVGVPFITAYEGLRRAGMPKAGSVVLVGGGNGKVGQATIQIATALGARVFAVERGAEPYRGHASGPVRMIDASREGIADVVRAETGGHGADIVYNTVGSPYFEQANLAMAIGATQIFISTIEKPVPFDIFAFYRGQHTYVGVDTLALDSQACANILDALAPMFASGALKPFPVLDDYTYALADAREAYRAVLQGASERVVLKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="68" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LMB5</accession>
    <name>Q1LMB5_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_1832</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08711.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516561.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1832"/>
    <dbReference type="EnsemblBacteria" id="ABF08711">
        <property type="protein sequence ID" value="ABF08711"/>
        <property type="gene ID" value="Rmet_1832"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1832"/>
    <dbReference type="eggNOG" id="COG3332">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047037_1_1_4"/>
    <dbReference type="OMA" id="TFTERNM"/>
    <dbReference type="OrthoDB" id="1721287at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008551">
        <property type="entry name" value="TANGO2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17985">
        <property type="entry name" value="PTHR17985"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05742">
        <property type="entry name" value="TANGO2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF08711.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="284" mass="31293" checksum="2FED28ED7EDDDC28" modified="2006-05-30" version="1">MCLILVAWQSHPDYPLVVAANRDEFYARPAAPADWWRDAPGVLGGRDLAEVIGDAGTWMGIAGAQHGLDSPVGARFAALTNYRAPSEKRTDARSRGELVAHFLRGDQTPADYLHDLAGDHGAYNGFNLLTSDLHDLWWYSNRSKSRVPQRLTPGLYGLSNALLDTPWPKVRSRVGAMCEVLAADRGQIGSNVESYLQLLADDRQAPDWELPSTGVAPEWEKLLSSAFIRSPNYGTRASTVLRVMHDGRFDFVERSFDADGQTGEVSYRGRLNLARDTGTVPAPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="106" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LMJ6</accession>
    <name>Q1LMJ6_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">DNA-binding response regulator in two-component regulatory system with AgrS</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">agrR</name>
        <name evidence="5" type="ordered locus">Rmet_1751</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08630.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516482.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1751"/>
    <dbReference type="EnsemblBacteria" id="ABF08630">
        <property type="protein sequence ID" value="ABF08630"/>
        <property type="gene ID" value="Rmet_1751"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1751"/>
    <dbReference type="eggNOG" id="COG0745">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_30_1_4"/>
    <dbReference type="OMA" id="VCMSKLR"/>
    <dbReference type="OrthoDB" id="1020672at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00383">
        <property type="entry name" value="trans_reg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001867">
        <property type="entry name" value="OmpR/PhoB-type_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006291">
        <property type="entry name" value="PcoR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039420">
        <property type="entry name" value="WalR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48111">
        <property type="entry name" value="PTHR48111"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00486">
        <property type="entry name" value="Trans_reg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00862">
        <property type="entry name" value="Trans_reg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01387">
        <property type="entry name" value="cztR_silR_copR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51755">
        <property type="entry name" value="OMPR_PHOB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Response regulatory" evidence="3">
        <location>
            <begin position="2"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="domain" description="OmpR/PhoB-type" evidence="4">
        <location>
            <begin position="123"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="OmpR/PhoB-type" evidence="2">
        <location>
            <begin position="123"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="1">
        <location>
            <position position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABF08630.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="224" mass="25594" checksum="227B74F936D71A3A" modified="2006-05-30" version="1">MKILVIEDEPKTGEYLRKGLTESSFVVDLASTGRDGLHHAIDGDYDLVILDVMLPGMNGWEVLKELREHKDTPVLFLTARDEVEDRVKGLELGGDDYLAKPFAFVELLARARTLLRRGPTREAERIEIEDLEIDLLRHRVSRGGIRIDLSPREFALLHFLARRHGEVLSRTQIASHVWDMNFDSDTNVVDVAIRRLRAKMDDTYAPKLIHSVRGIGYVLENRQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="100" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LMP7</accession>
    <name>Q1LMP7_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Two component response regulator, LuxR family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Rmet_1698</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08579.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1698"/>
    <dbReference type="EnsemblBacteria" id="ABF08579">
        <property type="protein sequence ID" value="ABF08579"/>
        <property type="gene ID" value="Rmet_1698"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1698"/>
    <dbReference type="eggNOG" id="COG4566">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_90_4_4"/>
    <dbReference type="OMA" id="DACEGGR"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06170">
        <property type="entry name" value="LuxR_C_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016032">
        <property type="entry name" value="Sig_transdc_resp-reg_C-effctor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000792">
        <property type="entry name" value="Tscrpt_reg_LuxR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00196">
        <property type="entry name" value="GerE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00038">
        <property type="entry name" value="HTHLUXR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00421">
        <property type="entry name" value="HTH_LUXR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46894">
        <property type="entry name" value="SSF46894"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50043">
        <property type="entry name" value="HTH_LUXR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Response regulatory" evidence="4">
        <location>
            <begin position="1"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="domain" description="HTH luxR-type" evidence="3">
        <location>
            <begin position="119"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="2">
        <location>
            <position position="41"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABF08579.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="191" mass="21298" checksum="69CA256237C51BB3" modified="2006-05-30" version="1">MRRALARVLGAHRYHTSCFDSAKRFLDNVELENSPICAVVDLQMPDMDGIALQQTLSQRVPVVILTGHADVSKAVQAMQAGAIDFLEKPVQDGLLLAAVERACERAREQFARKAELRVLRQKIERLTPREREVLGWIVSGLRNKQVASELGTVEKTIKVHRAHVMQKLEVDSLPALVRIADKMGMGIVART</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-10-07" version="66" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LMS0</accession>
    <name>Q1LMS0_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative transmembrane protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_1675</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08556.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1675"/>
    <dbReference type="EnsemblBacteria" id="ABF08556">
        <property type="protein sequence ID" value="ABF08556"/>
        <property type="gene ID" value="Rmet_1675"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1675"/>
    <dbReference type="eggNOG" id="ENOG5031WM6">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033800_1_0_4"/>
    <dbReference type="OMA" id="GEGPWYR"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025060">
        <property type="entry name" value="DUF3999"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13163">
        <property type="entry name" value="DUF3999"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1 3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193339" evidence="2">
        <location>
            <begin position="23"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="407"/>
            <end position="428"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF08556.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="442" mass="47285" checksum="F060D4AEF6D95D11" modified="2006-05-30" version="1">MIRSVGFCWLCMCLLLAPQAHAERFVVETFTAAPYFTLTLDARVYTHARGGLADLRVLNGAGEALPYALAVPRAEAVPMRTLHDVPWFPAPAAASNRAESGLGVVIGADGTLHAAGQAPGAKTTADWLLDLSRLTEPAAAVQLGLGDSDFEGAVAVQASDDLQAWQPAGTARILRVSRGDSQLLQERIALEARRTRYLRLQWQGAAPEVKGVQVETVTAQPAPPAPLQWSQGVPGVPDQAGGYSFDLAGRFPVEQVRVGLPQVNTITQATLWSRRDAQARWEHVSDLRLFRLSGPNGEQANESVTVPRNDDRYWRLSVDPRSGGLGDGRPLLFVGWQPAELTFVARGAPPFQVVVGEATEGTVRLSKADLLAGTTPAIGQARLGTMQASPAGLVSPQVPRREAWRKAVLWAALVAAVMVLGAMAWFLLRQSGRSDEKGGAGH</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-10-07" version="50" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LMT5</accession>
    <name>Q1LMT5_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_1658</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08541.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF08541">
        <property type="protein sequence ID" value="ABF08541"/>
        <property type="gene ID" value="Rmet_1658"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1658"/>
    <dbReference type="eggNOG" id="ENOG502ZRYD">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1433395_0_0_4"/>
    <dbReference type="OMA" id="LIAYPRR"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF08541.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="189" mass="20316" checksum="E9D5E02F795B4844" modified="2006-05-30" version="1">MSGGLRQSFPHPLTESIDLARQRRPSRYRARAFSVPPMYSRRQWQCREQGADMNRLIAYPRRMGALLLSICALPVASHAESGVIRFSGRIVAPPYEIRAVPAAIVRTAALETSGADLVFSSSPSNRPSAKLQVEGLPEHAPLTLRYLDAKGAATALLPGTSQRIGPNGGTLFVTSGPGFASALVSVTYN</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="54" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LMV0</accession>
    <name>Q1LMV0_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_1643</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08526.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516371.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1643"/>
    <dbReference type="EnsemblBacteria" id="ABF08526">
        <property type="protein sequence ID" value="ABF08526"/>
        <property type="gene ID" value="Rmet_1643"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1643"/>
    <dbReference type="HOGENOM" id="CLU_1822171_0_0_4"/>
    <dbReference type="OMA" id="TEYWSRA"/>
    <dbReference type="OrthoDB" id="2167556at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193342" evidence="1">
        <location>
            <begin position="20"/>
            <end position="149"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF08526.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="149" mass="16967" checksum="21CC1D24C87171EC" modified="2006-05-30" version="1">MSRLLAVMLLTLTATGAGAATAVTKCESSQDTLYTATPSCPAGYTNTTRTMRGSVVTVPRATPKARQAEMDYLQHQAQISQQIQAWDERDAEQDWRLMNVFWNQCRVLDYQIRGTEWAMEHTEYWSRADRYREAVNAARATQFQMGCFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LN09</accession>
    <name>Q1LN09_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_1584</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PrpF family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08467.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516319.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1584"/>
    <dbReference type="EnsemblBacteria" id="ABF08467">
        <property type="protein sequence ID" value="ABF08467"/>
        <property type="gene ID" value="Rmet_1584"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1584"/>
    <dbReference type="eggNOG" id="COG2828">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026443_2_0_4"/>
    <dbReference type="OMA" id="VPATYMR"/>
    <dbReference type="OrthoDB" id="1328531at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016853">
        <property type="term" value="F:isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019629">
        <property type="term" value="P:propionate catabolic process, 2-methylcitrate cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012709">
        <property type="entry name" value="PrpF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007400">
        <property type="entry name" value="PrpF_protein"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43709">
        <property type="entry name" value="PTHR43709"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04303">
        <property type="entry name" value="PrpF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02334">
        <property type="entry name" value="prpF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0413">Isomerase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF08467.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="397" mass="41637" checksum="99305DEA39E61A5F" modified="2006-05-30" version="1">MAHVPQIRIPATYMRGGTSKGVFFRVQDLPEAAQVPGKARDALLMRVIGSPDPYGKQIDGMGAATSSTSKTVLISKSTRPDHDVDYLFGQVSIDQPFVDWSGNCGNLSAAVGPYAISNGFIDAARIPENGTVTVRIWQANIGKTIISHVPITNGAVQETGDFELDGVTFPAAEVQLEFMDPAAEEGDDGGGAMFPTGNLVDDLEVPGVGTLKATMINAGIPTIFVNAEAIGYTGTELQEAINGDPKALAMFETIRAYGAKRMGLIRDIDEAARRQHTPKVAFVAKPATYTASSGKTVNAGDVDLLVRALSMGKLHHAMMGTAAVAIGTAAAVPGTLVNLAAGGGEREAVRFGHPSGTLRVGAQAVQSDGEWTVTKAIMSRSARVLMEGWVRIPGDAF</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LN11</accession>
    <name>Q1LN11_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">4HBT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_1582</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08465.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516317.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1582"/>
    <dbReference type="EnsemblBacteria" id="ABF08465">
        <property type="protein sequence ID" value="ABF08465"/>
        <property type="gene ID" value="Rmet_1582"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1582"/>
    <dbReference type="eggNOG" id="COG2050">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_089876_5_0_4"/>
    <dbReference type="OMA" id="NVTHGSI"/>
    <dbReference type="OrthoDB" id="1814691at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029069">
        <property type="entry name" value="HotDog_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003736">
        <property type="entry name" value="PAAI_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006683">
        <property type="entry name" value="Thioestr_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03061">
        <property type="entry name" value="4HBT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54637">
        <property type="entry name" value="SSF54637"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00369">
        <property type="entry name" value="unchar_dom_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="4HBT" evidence="1">
        <location>
            <begin position="57"/>
            <end position="133"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF08465.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="146" mass="14986" checksum="8EE17AA3B899E9C1" modified="2006-05-30" version="1">MSESANAVDPRNAAMIESVLIASPVAQALGIRLDALGAERVEMLLPFSMKNITVGDIVHAGVIATLIDVAGAAAAFSKVDLDVVKTGATGSLSIQYLAPARAASLRAVAEVIRRGKRQVATEVSVYAEGDAEAVLVAKALMSTALI</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="106" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LN80</accession>
    <name>Q1LN80_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 8">Transketolase</fullName>
            <ecNumber evidence="3 8">2.2.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">cbbT1</name>
        <name evidence="10" type="ordered locus">Rmet_1513</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Catalyzes the transfer of a two-carbon ketol group from a ketose donor to an aldose acceptor, via a covalent intermediate with the cofactor thiamine pyrophosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>D-glyceraldehyde 3-phosphate + D-sedoheptulose 7-phosphate = aldehydo-D-ribose 5-phosphate + D-xylulose 5-phosphate</text>
            <dbReference type="Rhea" id="RHEA:10508"/>
            <dbReference type="ChEBI" id="CHEBI:57483"/>
            <dbReference type="ChEBI" id="CHEBI:57737"/>
            <dbReference type="ChEBI" id="CHEBI:58273"/>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
            <dbReference type="EC" id="2.2.1.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <cofactor evidence="8">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
        <cofactor evidence="8">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <cofactor evidence="8">
            <name>Co(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:48828"/>
        </cofactor>
        <text evidence="8">Binds 1 Mg(2+) ion per subunit. Can also utilize other divalent metal cations, such as Ca(2+), Mn(2+) and Co(2+).</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>thiamine diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58937"/>
        </cofactor>
        <text evidence="8">Binds 1 thiamine pyrophosphate per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2 8">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the transketolase family.</text>
    </comment>
    <dbReference type="EC" id="2.2.1.1" evidence="3 8"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08396.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516256.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1513"/>
    <dbReference type="EnsemblBacteria" id="ABF08396">
        <property type="protein sequence ID" value="ABF08396"/>
        <property type="gene ID" value="Rmet_1513"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1513"/>
    <dbReference type="eggNOG" id="COG0021">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009227_0_0_4"/>
    <dbReference type="OMA" id="YALQQTD"/>
    <dbReference type="OrthoDB" id="188169at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004802">
        <property type="term" value="F:transketolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd02012">
        <property type="entry name" value="TPP_TK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009014">
        <property type="entry name" value="Transketo_C/PFOR_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005475">
        <property type="entry name" value="Transketolase-like_Pyr-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005478">
        <property type="entry name" value="Transketolase_bac-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020826">
        <property type="entry name" value="Transketolase_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033248">
        <property type="entry name" value="Transketolase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033247">
        <property type="entry name" value="Transketolase_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005474">
        <property type="entry name" value="Transketolase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43522">
        <property type="entry name" value="PTHR43522"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02779">
        <property type="entry name" value="Transket_pyr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02780">
        <property type="entry name" value="Transketolase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00456">
        <property type="entry name" value="Transketolase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00861">
        <property type="entry name" value="Transket_pyr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="SSF52518"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52922">
        <property type="entry name" value="SSF52922"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00232">
        <property type="entry name" value="tktlase_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00801">
        <property type="entry name" value="TRANSKETOLASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00802">
        <property type="entry name" value="TRANSKETOLASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0106">Calcium</keyword>
    <keyword evidence="6 8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 8" id="KW-0786">Thiamine pyrophosphate</keyword>
    <keyword evidence="4 8 10" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="TRANSKETOLASE_1" evidence="9">
        <location>
            <begin position="25"/>
            <end position="45"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007131"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU004996"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS00801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ABF08396.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="684" mass="73482" checksum="3BFEEA836D9D886F" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LNB5</accession>
    <name>Q1LNB5_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">ParB nuclease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">parB</name>
        <name evidence="4" type="ordered locus">Rmet_1478</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08361.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516227.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1478"/>
    <dbReference type="EnsemblBacteria" id="ABF08361">
        <property type="protein sequence ID" value="ABF08361"/>
        <property type="gene ID" value="Rmet_1478"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1478"/>
    <dbReference type="eggNOG" id="COG1475">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019174_1_0_4"/>
    <dbReference type="OMA" id="YEKPNPY"/>
    <dbReference type="OrthoDB" id="1375763at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003115">
        <property type="entry name" value="ParB/Sulfiredoxin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036086">
        <property type="entry name" value="ParB/Sulfiredoxin_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02195">
        <property type="entry name" value="ParBc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00470">
        <property type="entry name" value="ParB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110849">
        <property type="entry name" value="SSF110849"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ParB" evidence="3">
        <location>
            <begin position="30"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="652"/>
            <end position="680"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="321"/>
            <end position="348"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00470"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF08361.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="680" mass="73328" checksum="A3E1B707108EC2F9" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="70" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LND6</accession>
    <name>Q1LND6_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Predicted oligoketide cyclase/lipid transport protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">yfjG</name>
        <name evidence="3" type="ordered locus">Rmet_1457</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ribosome association toxin RatA family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08340.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516206.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1457"/>
    <dbReference type="EnsemblBacteria" id="ABF08340">
        <property type="protein sequence ID" value="ABF08340"/>
        <property type="gene ID" value="Rmet_1457"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1457"/>
    <dbReference type="eggNOG" id="COG2867">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_079653_3_1_4"/>
    <dbReference type="OMA" id="YIDGPFK"/>
    <dbReference type="OrthoDB" id="1691158at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048039">
        <property type="term" value="F:ubiquinone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045333">
        <property type="term" value="P:cellular respiration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006744">
        <property type="term" value="P:ubiquinone biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07813">
        <property type="entry name" value="COQ10p_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.530.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044996">
        <property type="entry name" value="COQ10-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005031">
        <property type="entry name" value="COQ10_START"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023393">
        <property type="entry name" value="START-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12901">
        <property type="entry name" value="PTHR12901"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03364">
        <property type="entry name" value="Polyketide_cyc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Polyketide_cyc" evidence="2">
        <location>
            <begin position="11"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF03364"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF08340.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="149" mass="17174" checksum="E87FE37F272EF63B" modified="2006-05-30" version="1">MADVHKSVLLGHSAEQMYDLVTRVEDYPKFLPWCGGVEVFEQTDTLLDAKIHIHFKGIKQFFHTRNAQERPTRIDMTFADGPFKVFNGSWRFTPLREDACKIEFHLHYEFTSVFLEKIIGPVFSVIANTFVDAFVKRAEVVYANNANNG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-02-05" modified="2022-08-03" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LNE1</accession>
    <name>PSRP_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Putative phosphoenolpyruvate synthase regulatory protein</fullName>
            <shortName evidence="1">PEP synthase regulatory protein</shortName>
            <shortName evidence="1">PSRP</shortName>
            <ecNumber evidence="1">2.7.11.33</ecNumber>
            <ecNumber evidence="1">2.7.4.28</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Pyruvate, water dikinase regulatory protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="ordered locus">Rmet_1452</name>
    </gene>
    <organism>
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Bifunctional serine/threonine kinase and phosphorylase involved in the regulation of the phosphoenolpyruvate synthase (PEPS) by catalyzing its phosphorylation/dephosphorylation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>[pyruvate, water dikinase] + ADP = [pyruvate, water dikinase]-phosphate + AMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46020"/>
            <dbReference type="Rhea" id="RHEA-COMP:11425"/>
            <dbReference type="Rhea" id="RHEA-COMP:11426"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43176"/>
            <dbReference type="ChEBI" id="CHEBI:68546"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.33"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>[pyruvate, water dikinase]-phosphate + H(+) + phosphate = [pyruvate, water dikinase] + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:48580"/>
            <dbReference type="Rhea" id="RHEA-COMP:11425"/>
            <dbReference type="Rhea" id="RHEA-COMP:11426"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:43176"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:68546"/>
            <dbReference type="EC" id="2.7.4.28"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the pyruvate, phosphate/water dikinase regulatory protein family. PSRP subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.33" evidence="1"/>
    <dbReference type="EC" id="2.7.4.28" evidence="1"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08335.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516201.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q1LNE1"/>
    <dbReference type="SMR" id="Q1LNE1"/>
    <dbReference type="STRING" id="266264.Rmet_1452"/>
    <dbReference type="EnsemblBacteria" id="ABF08335">
        <property type="protein sequence ID" value="ABF08335"/>
        <property type="gene ID" value="Rmet_1452"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1452"/>
    <dbReference type="eggNOG" id="COG1806">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046206_1_0_4"/>
    <dbReference type="OMA" id="YAQCEFE"/>
    <dbReference type="OrthoDB" id="980472at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043531">
        <property type="term" value="F:ADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016776">
        <property type="term" value="F:phosphotransferase activity, phosphate group as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006470">
        <property type="term" value="P:protein dephosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006468">
        <property type="term" value="P:protein phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01062">
        <property type="entry name" value="PSRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005177">
        <property type="entry name" value="Kinase-pyrophosphorylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026530">
        <property type="entry name" value="PSRP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31756">
        <property type="entry name" value="PTHR31756"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03618">
        <property type="entry name" value="Kinase-PPPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0418">Kinase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000316721" description="Putative phosphoenolpyruvate synthase regulatory protein">
        <location>
            <begin position="1"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="150"/>
            <end position="157"/>
        </location>
        <ligand>
            <name>ADP</name>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01062"/>
        </source>
    </evidence>
    <sequence length="270" mass="30653" checksum="B1817717F32397C4" modified="2006-05-30" version="1">MRTVFIVSDGTGITAETFSHSILAQFEMRFRKVRMPFVDTPDKAHVAVGKINEAFYNEGVPPIVFTTLVNAESNKAIRRAKAMILDMFQTFIEPLEKELGLKSTHAIGRFHQNADTEAYKNRIEAINFSLAHDDGQSHKNLEDADVILVGVSRSGKTPTSLYLAMQYGLKAANYPLIPDDFERGRLPSALYAYKQKIFGLSIDPQRLTEIRNERRPGSKYAAVENCRYEVNEAESMMRREGIKWLSSTHKSIEEIATTILQEIKVDRDNY</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LNL0</accession>
    <name>Q1LNL0_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">GGACT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">ytfP</name>
        <name evidence="2" type="ordered locus">Rmet_1383</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08266.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516144.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1383"/>
    <dbReference type="EnsemblBacteria" id="ABF08266">
        <property type="protein sequence ID" value="ABF08266"/>
        <property type="gene ID" value="Rmet_1383"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1383"/>
    <dbReference type="eggNOG" id="COG2105">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_083466_3_0_4"/>
    <dbReference type="OMA" id="LYDFGTY"/>
    <dbReference type="OrthoDB" id="1984556at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd06661">
        <property type="entry name" value="GGCT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009288">
        <property type="entry name" value="AIG2-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013024">
        <property type="entry name" value="GGCT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036568">
        <property type="entry name" value="GGCT-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06094">
        <property type="entry name" value="GGACT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110857">
        <property type="entry name" value="SSF110857"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GGACT" evidence="1">
        <location>
            <begin position="4"/>
            <end position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF06094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF08266.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="138" mass="14452" checksum="6AE4B900A272AAD4" modified="2006-05-30" version="1">MPRVFVYGTLRAGEVNDLNRAATRHGIEAPTLVGTGTIAGRLYDFGTYPGLVLDAAAGPVAGDIYDIPDALLPVLDEIEEVYPGQATLFVRETQPVALAGAATDCLLYPVADSAVTGLVCIDSGDWVAYRRARDAAAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LP87</accession>
    <name>Q1LP87_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Acetyl-CoA:acetoacetyl-CoA transferase, alpha subunit</fullName>
            <ecNumber evidence="3">2.8.3.8</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">atoD</name>
        <name evidence="3" type="ordered locus">Rmet_1153</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the 3-oxoacid CoA-transferase subunit A family.</text>
    </comment>
    <dbReference type="EC" id="2.8.3.8" evidence="3"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08039.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008650509.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1153"/>
    <dbReference type="EnsemblBacteria" id="ABF08039">
        <property type="protein sequence ID" value="ABF08039"/>
        <property type="gene ID" value="Rmet_1153"/>
    </dbReference>
    <dbReference type="GeneID" id="60822022"/>
    <dbReference type="KEGG" id="rme:Rmet_1153"/>
    <dbReference type="eggNOG" id="COG1788">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019942_2_0_4"/>
    <dbReference type="OMA" id="FVKAWKG"/>
    <dbReference type="OrthoDB" id="1660857at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008775">
        <property type="term" value="F:acetate CoA-transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012792">
        <property type="entry name" value="3-oxoacid_CoA-transf_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004165">
        <property type="entry name" value="CoA_trans_fam_I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004163">
        <property type="entry name" value="CoA_transf_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037171">
        <property type="entry name" value="NagB/RpiA_transferase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13707">
        <property type="entry name" value="PTHR13707"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01144">
        <property type="entry name" value="CoA_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00882">
        <property type="entry name" value="CoA_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100950">
        <property type="entry name" value="SSF100950"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02429">
        <property type="entry name" value="pcaI_scoA_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01273">
        <property type="entry name" value="COA_TRANSF_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005612"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF08039.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="232" mass="24953" checksum="C6EB936844A29E80" modified="2006-05-30" version="1">MNKVYASAAEALAGVVRDGQTIAVGGFGLCGIPEALIAALRDSGAKQLTCISNNAGVDGFGLGLLLATRQIRKMISSYVGENKEFERQYLAGELELEFTPQGTLAEKLRAGGSGIPAFFTKTGVGTIVADGKEIREFDGQQYVMERSLTADVALVKAKKADKAGNLVFRRTARNFNPVCAMAGKITVVEVEEIVETGEIDPDDVHLPGIFVHRIVLNTNPEKRIEQRTVRAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LPB9</accession>
    <name>Q1LPB9_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Efflux pump membrane transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">acrD</name>
        <name evidence="5" type="ordered locus">Rmet_1121</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 4">Cell inner membrane</location>
            <topology evidence="2 4">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 4">Belongs to the resistance-nodulation-cell division (RND) (TC 2.A.6) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08007.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515908.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1121"/>
    <dbReference type="EnsemblBacteria" id="ABF08007">
        <property type="protein sequence ID" value="ABF08007"/>
        <property type="gene ID" value="Rmet_1121"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1121"/>
    <dbReference type="eggNOG" id="COG0841">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002755_1_1_4"/>
    <dbReference type="OMA" id="APAMCAS"/>
    <dbReference type="OrthoDB" id="145346at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015562">
        <property type="term" value="F:efflux transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042908">
        <property type="term" value="P:xenobiotic transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2090.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027463">
        <property type="entry name" value="AcrB_DN_DC_subdom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001036">
        <property type="entry name" value="Acrflvin-R"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004764">
        <property type="entry name" value="HAE1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32063">
        <property type="entry name" value="PTHR32063"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00873">
        <property type="entry name" value="ACR_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00702">
        <property type="entry name" value="ACRIFLAVINRP"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82714">
        <property type="entry name" value="SSF82714"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00915">
        <property type="entry name" value="2A0602"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="339"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="365"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="391"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="440"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="472"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="536"/>
            <end position="553"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="867"/>
            <end position="886"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="893"/>
            <end position="913"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="919"/>
            <end position="943"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="964"/>
            <end position="984"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="996"/>
            <end position="1022"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004429"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU364070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABF08007.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="1044" mass="112058" checksum="5D0CAD4224E2319B" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LPQ9</accession>
    <name>Q1LPQ9_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">N-Formylglutamate amidohydrolase</fullName>
            <ecNumber evidence="1">3.5.1.68</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">hutG</name>
        <name evidence="1" type="ordered locus">Rmet_0981</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EC" id="3.5.1.68" evidence="1"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07867.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515785.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0981"/>
    <dbReference type="EnsemblBacteria" id="ABF07867">
        <property type="protein sequence ID" value="ABF07867"/>
        <property type="gene ID" value="Rmet_0981"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0981"/>
    <dbReference type="eggNOG" id="COG3741">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069318_1_0_4"/>
    <dbReference type="OMA" id="NHPYKGV"/>
    <dbReference type="OrthoDB" id="813848at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050129">
        <property type="term" value="F:N-formylglutamate deformylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007709">
        <property type="entry name" value="N-FG_amidohydro"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05013">
        <property type="entry name" value="FGase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF07867.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="290" mass="32905" checksum="46CB00AAFDF47266" modified="2006-05-30" version="1">MTEADRKTAESPYFLHMPEGECGPLFLDSPHSCTTYPADFRFAGNLPLPLLRQAEDTFVDELYADAPARGIPLLCAGFPRSYLDANRGLEEIDEALLDAPWPGAKQETSKTRLGKGLVWRVLDTGEPIYDRKLGVAELQARIDNYYLPYYAQLQRLSDQAVARHGRVWHINCHSMPSEAAQYATEHPGLQHPDFVVGDRDGTTCDTRFTDRVESVLKEMGYDVWRNHPYKGVEIVRVVGQPQANRHSLQLEVNRKLYMDEVGLTHSAGFDKLKRDLNALMDELLRFARTI</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-12-16" modified="2022-05-25" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LPW1</accession>
    <name>NUOC_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">NADH-quinone oxidoreductase subunit C</fullName>
            <ecNumber evidence="1">7.1.1.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">NADH dehydrogenase I subunit C</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">NDH-1 subunit C</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">nuoC</name>
        <name type="ordered locus">Rmet_0929</name>
    </gene>
    <organism>
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a quinone + 5 H(+)(in) + NADH = a quinol + 4 H(+)(out) + NAD(+)</text>
            <dbReference type="Rhea" id="RHEA:57888"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:24646"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:132124"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">NDH-1 is composed of 14 different subunits. Subunits NuoB, C, D, E, F, and G constitute the peripheral sector of the complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell inner membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the complex I 30 kDa subunit family.</text>
    </comment>
    <dbReference type="EC" id="7.1.1.-" evidence="1"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07815.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008643343.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q1LPW1"/>
    <dbReference type="SMR" id="Q1LPW1"/>
    <dbReference type="STRING" id="266264.Rmet_0929"/>
    <dbReference type="EnsemblBacteria" id="ABF07815">
        <property type="protein sequence ID" value="ABF07815"/>
        <property type="gene ID" value="Rmet_0929"/>
    </dbReference>
    <dbReference type="GeneID" id="60825416"/>
    <dbReference type="KEGG" id="rme:Rmet_0929"/>
    <dbReference type="eggNOG" id="COG0852">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_042628_2_1_4"/>
    <dbReference type="OMA" id="MERETYD"/>
    <dbReference type="OrthoDB" id="1735902at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008137">
        <property type="term" value="F:NADH dehydrogenase (ubiquinone) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048038">
        <property type="term" value="F:quinone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.460.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01357">
        <property type="entry name" value="NDH1_NuoC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010218">
        <property type="entry name" value="NADH_DH_suC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037232">
        <property type="entry name" value="NADH_quin_OxRdtase_su_C/D-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001268">
        <property type="entry name" value="NADH_UbQ_OxRdtase_30kDa_su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020396">
        <property type="entry name" value="NADH_UbQ_OxRdtase_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00329">
        <property type="entry name" value="Complex1_30kDa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143243">
        <property type="entry name" value="SSF143243"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01961">
        <property type="entry name" value="NuoC_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00542">
        <property type="entry name" value="COMPLEX1_30K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0997">Cell inner membrane</keyword>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-0520">NAD</keyword>
    <keyword id="KW-0874">Quinone</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-1278">Translocase</keyword>
    <keyword id="KW-0813">Transport</keyword>
    <keyword id="KW-0830">Ubiquinone</keyword>
    <feature type="chain" id="PRO_0000358175" description="NADH-quinone oxidoreductase subunit C">
        <location>
            <begin position="1"/>
            <end position="199"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01357"/>
        </source>
    </evidence>
    <sequence length="199" mass="23007" checksum="DB2BFBD1919724CD" modified="2006-05-30" version="1">MANLDTLKAALEKVLGKRVQNLIEATGELTLIVKAADYLEVARLLRDDPSLRFEQLLDLCGVDYSDYAEGAWDGLRFAAVSQLLSVTHNWRLRLRVFAPDDDFPVLPSVINVWNSVNWFEREAFDFYGIVFEGHPDLRRILTDYGFVGHPFRKDFPVSGYVEMRYDPEQKRVIYQPVTIEPREVTPRVIREENYGGTQH</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-04-29" modified="2022-08-03" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LPW7</accession>
    <name>PNP_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Polyribonucleotide nucleotidyltransferase</fullName>
            <ecNumber evidence="1">2.7.7.8</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Polynucleotide phosphorylase</fullName>
            <shortName evidence="1">PNPase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">pnp</name>
        <name type="ordered locus">Rmet_0923</name>
    </gene>
    <organism>
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in mRNA degradation. Catalyzes the phosphorolysis of single-stranded polyribonucleotides processively in the 3'- to 5'-direction.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>phosphate + RNA(n+1) = a ribonucleoside 5'-diphosphate + RNA(n)</text>
            <dbReference type="Rhea" id="RHEA:22096"/>
            <dbReference type="Rhea" id="RHEA-COMP:14527"/>
            <dbReference type="Rhea" id="RHEA-COMP:17342"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57930"/>
            <dbReference type="ChEBI" id="CHEBI:140395"/>
            <dbReference type="EC" id="2.7.7.8"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the polyribonucleotide nucleotidyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.8" evidence="1"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07809.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515744.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q1LPW7"/>
    <dbReference type="SMR" id="Q1LPW7"/>
    <dbReference type="STRING" id="266264.Rmet_0923"/>
    <dbReference type="EnsemblBacteria" id="ABF07809">
        <property type="protein sequence ID" value="ABF07809"/>
        <property type="gene ID" value="Rmet_0923"/>
    </dbReference>
    <dbReference type="GeneID" id="60825409"/>
    <dbReference type="KEGG" id="rme:Rmet_0923"/>
    <dbReference type="eggNOG" id="COG1185">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004217_2_2_4"/>
    <dbReference type="OMA" id="LHILDVM"/>
    <dbReference type="OrthoDB" id="122725at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004654">
        <property type="term" value="F:polyribonucleotide nucleotidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006402">
        <property type="term" value="P:mRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006396">
        <property type="term" value="P:RNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.70">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01595">
        <property type="entry name" value="PNPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001247">
        <property type="entry name" value="ExoRNase_PH_dom1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015847">
        <property type="entry name" value="ExoRNase_PH_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036345">
        <property type="entry name" value="ExoRNase_PH_dom2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004087">
        <property type="entry name" value="KH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004088">
        <property type="entry name" value="KH_dom_type_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036612">
        <property type="entry name" value="KH_dom_type_1_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012162">
        <property type="entry name" value="PNPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027408">
        <property type="entry name" value="PNPase/RNase_PH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015848">
        <property type="entry name" value="PNPase_PH_RNA-bd_bac/org-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036456">
        <property type="entry name" value="PNPase_PH_RNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022967">
        <property type="entry name" value="S1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003029">
        <property type="entry name" value="S1_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11252">
        <property type="entry name" value="PTHR11252"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00013">
        <property type="entry name" value="KH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03726">
        <property type="entry name" value="PNPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01138">
        <property type="entry name" value="RNase_PH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03725">
        <property type="entry name" value="RNase_PH_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00575">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005499">
        <property type="entry name" value="PNPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00322">
        <property type="entry name" value="KH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00316">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46915">
        <property type="entry name" value="SSF46915"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="SSF54211"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54791">
        <property type="entry name" value="SSF54791"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55666">
        <property type="entry name" value="SSF55666"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03591">
        <property type="entry name" value="polynuc_phos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50084">
        <property type="entry name" value="KH_TYPE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50126">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000329796" description="Polyribonucleotide nucleotidyltransferase">
        <location>
            <begin position="1"/>
            <end position="725"/>
        </location>
    </feature>
    <feature type="domain" description="KH" evidence="1">
        <location>
            <begin position="555"/>
            <end position="614"/>
        </location>
    </feature>
    <feature type="domain" description="S1 motif" evidence="1">
        <location>
            <begin position="624"/>
            <end position="692"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="702"/>
            <end position="725"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="488"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="494"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01595"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="725" mass="77832" checksum="9AC9A031A656DF18" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-12-02" version="106" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LQ57</accession>
    <name>Q1LQ57_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Transcriptional regulator (GntR family)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Rmet_0833</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07719.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0833"/>
    <dbReference type="EnsemblBacteria" id="ABF07719">
        <property type="protein sequence ID" value="ABF07719"/>
        <property type="gene ID" value="Rmet_0833"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0833"/>
    <dbReference type="eggNOG" id="COG2186">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017584_9_1_4"/>
    <dbReference type="OMA" id="ETDTTAW"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07377">
        <property type="entry name" value="WHTH_GntR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.530">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011711">
        <property type="entry name" value="GntR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008920">
        <property type="entry name" value="TF_FadR/GntR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000524">
        <property type="entry name" value="Tscrpt_reg_HTH_GntR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07729">
        <property type="entry name" value="FCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00392">
        <property type="entry name" value="GntR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00035">
        <property type="entry name" value="HTHGNTR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00895">
        <property type="entry name" value="FCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00345">
        <property type="entry name" value="HTH_GNTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48008">
        <property type="entry name" value="SSF48008"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50949">
        <property type="entry name" value="HTH_GNTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH gntR-type" evidence="5">
        <location>
            <begin position="40"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABF07719.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="272" mass="29927" checksum="C7A72C9B578285BC" modified="2006-05-30" version="1">MSASLHGQQPVIATGQDPRYLPAMSNAPLTSPAPLRRRGRTLAEEVVGSLTENIQQGQLKPGDKLPTESEIMARLGVSRTVVREALSRLQASGMVETRHGIGTFVLNRPAETQSPFRIDPSAIVTAMDVLAMLEFRVSLEAEAASLAATRRSDDQLRAIRRALDDIKHSALEGSDAVGADFEFHLSIARATGNRYFTDIMGHLGTMVIPRSRLQVSQPEREQYLERVNFEHESIYDAIERRDPEAAKAAMRMHLTNSRERLRKASAMAGSAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="100" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LR35</accession>
    <name>Q1LR35_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 4">N5-carboxyaminoimidazole ribonucleotide mutase</fullName>
            <shortName evidence="3 4">N5-CAIR mutase</shortName>
            <ecNumber evidence="3 4">5.4.99.18</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">5-(carboxyamino)imidazole ribonucleotide mutase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="3 7" type="primary">purE</name>
        <name evidence="7" type="ordered locus">Rmet_0505</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3 4">Catalyzes the conversion of N5-carboxyaminoimidazole ribonucleotide (N5-CAIR) to 4-carboxy-5-aminoimidazole ribonucleotide (CAIR).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3 4">
            <text>5-carboxyamino-1-(5-phospho-D-ribosyl)imidazole + H(+) = 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate</text>
            <dbReference type="Rhea" id="RHEA:13193"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:58730"/>
            <dbReference type="ChEBI" id="CHEBI:77657"/>
            <dbReference type="EC" id="5.4.99.18"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3 4">Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate from 5-amino-1-(5-phospho-D-ribosyl)imidazole (N5-CAIR route): step 2/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the AIR carboxylase family. Class I subfamily.</text>
    </comment>
    <dbReference type="EC" id="5.4.99.18" evidence="3 4"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07391.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008652146.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0505"/>
    <dbReference type="EnsemblBacteria" id="ABF07391">
        <property type="protein sequence ID" value="ABF07391"/>
        <property type="gene ID" value="Rmet_0505"/>
    </dbReference>
    <dbReference type="GeneID" id="60824102"/>
    <dbReference type="KEGG" id="rme:Rmet_0505"/>
    <dbReference type="eggNOG" id="COG0041">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_094982_2_2_4"/>
    <dbReference type="OMA" id="SNSIDGW"/>
    <dbReference type="OrthoDB" id="1701464at2"/>
    <dbReference type="UniPathway" id="UPA00074">
        <property type="reaction ID" value="UER00943"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034023">
        <property type="term" value="F:5-(carboxyamino)imidazole ribonucleotide mutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01929">
        <property type="entry name" value="PurE_classI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033747">
        <property type="entry name" value="PurE_ClassI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000031">
        <property type="entry name" value="PurE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024694">
        <property type="entry name" value="PurE_prokaryotes"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00731">
        <property type="entry name" value="AIRC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001338">
        <property type="entry name" value="AIR_carboxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01001">
        <property type="entry name" value="AIRC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01162">
        <property type="entry name" value="purE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3 4" id="KW-0413">Isomerase</keyword>
    <keyword evidence="7" id="KW-0456">Lyase</keyword>
    <keyword evidence="1 3 4" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AIRC" evidence="6">
        <location>
            <begin position="7"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3 5">
        <location>
            <position position="15"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3 5">
        <location>
            <position position="18"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3 5">
        <location>
            <position position="45"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRNR" id="PIRNR001338"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR001338-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM01001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABF07391.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="165" mass="17103" checksum="17B7B7DF47501BCD" modified="2006-05-30" version="1">MSNESKPVVGVVMGSSSDWDVMQHAVAMLKDFNVPFEAQVVSAHRMADDMFRYAEAARGRGIRAIIAGAGGAAHLPGMIAAKTIVPVFGVPVPSKYLRGEDSLLSIVQMPKGVPVATFAIGEAGAANAALHAIATLATTDDALASALEAFRAKQTEAARAMTLPV</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-01-15" modified="2022-05-25" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LR92</accession>
    <name>SYGA_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Glycine--tRNA ligase alpha subunit</fullName>
            <ecNumber evidence="1">6.1.1.14</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Glycyl-tRNA synthetase alpha subunit</fullName>
            <shortName evidence="1">GlyRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">glyQ</name>
        <name type="ordered locus">Rmet_0448</name>
    </gene>
    <organism>
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + glycine + tRNA(Gly) = AMP + diphosphate + glycyl-tRNA(Gly)</text>
            <dbReference type="Rhea" id="RHEA:16013"/>
            <dbReference type="Rhea" id="RHEA-COMP:9664"/>
            <dbReference type="Rhea" id="RHEA-COMP:9683"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78522"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.14"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Tetramer of two alpha and two beta subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-II aminoacyl-tRNA synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.14" evidence="1"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07334.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515322.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q1LR92"/>
    <dbReference type="SMR" id="Q1LR92"/>
    <dbReference type="STRING" id="266264.Rmet_0448"/>
    <dbReference type="EnsemblBacteria" id="ABF07334">
        <property type="protein sequence ID" value="ABF07334"/>
        <property type="gene ID" value="Rmet_0448"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0448"/>
    <dbReference type="eggNOG" id="COG0752">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_057066_1_0_4"/>
    <dbReference type="OMA" id="SYYQFQV"/>
    <dbReference type="OrthoDB" id="676868at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004820">
        <property type="term" value="F:glycine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006426">
        <property type="term" value="P:glycyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00733">
        <property type="entry name" value="GlyRS_alpha_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00254">
        <property type="entry name" value="Gly_tRNA_synth_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006194">
        <property type="entry name" value="Gly-tRNA-synth_heterodimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002310">
        <property type="entry name" value="Gly-tRNA_ligase_asu"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02091">
        <property type="entry name" value="tRNA-synt_2e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01044">
        <property type="entry name" value="TRNASYNTHGA"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="SSF55681"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00388">
        <property type="entry name" value="glyQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50861">
        <property type="entry name" value="AA_TRNA_LIGASE_II_GLYAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_1000047476" description="Glycine--tRNA ligase alpha subunit">
        <location>
            <begin position="1"/>
            <end position="317"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00254"/>
        </source>
    </evidence>
    <sequence length="317" mass="36365" checksum="54CE1AB6347075AB" modified="2006-05-30" version="1">MLTFQQMILTLQAYWDRQGCALLQPIDLEVGAGTSHVHTFLRAIGPEPWRAAYVQPSRRPKDGRYGENPNRLQHYYQYQVVLKPAPENILELYLGSLEALGLDLKQNDIRFVEDDWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGIDCKPITGEITYGIERLAMYLQKVENIYDLVWTEWQENGETRRLTYGDVYHQNEVEQSTYNFEHSNTEILFRHFAEHESEAKRLMGDTGEKAEGAQEKAAGTRLALPAYEQVLKAAHTFNLLDARGAISVTERAAYIGRIRNLSRQVAQAYYDSREALGFPMCKTEARA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-01-15" modified="2022-05-25" version="98" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LS94</accession>
    <name>SYR_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Arginine--tRNA ligase</fullName>
            <ecNumber evidence="1">6.1.1.19</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Arginyl-tRNA synthetase</fullName>
            <shortName evidence="1">ArgRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">argS</name>
        <name type="ordered locus">Rmet_0096</name>
    </gene>
    <organism>
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + L-arginine + tRNA(Arg) = AMP + diphosphate + L-arginyl-tRNA(Arg)</text>
            <dbReference type="Rhea" id="RHEA:20301"/>
            <dbReference type="Rhea" id="RHEA-COMP:9658"/>
            <dbReference type="Rhea" id="RHEA-COMP:9673"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:32682"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78513"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.19"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-I aminoacyl-tRNA synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.19" evidence="1"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF06982.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515017.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q1LS94"/>
    <dbReference type="SMR" id="Q1LS94"/>
    <dbReference type="STRING" id="266264.Rmet_0096"/>
    <dbReference type="EnsemblBacteria" id="ABF06982">
        <property type="protein sequence ID" value="ABF06982"/>
        <property type="gene ID" value="Rmet_0096"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0096"/>
    <dbReference type="eggNOG" id="COG0018">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006406_0_1_4"/>
    <dbReference type="OMA" id="NKPLHLG"/>
    <dbReference type="OrthoDB" id="1146366at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004814">
        <property type="term" value="F:arginine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006420">
        <property type="term" value="P:arginyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00671">
        <property type="entry name" value="ArgRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1360.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00123">
        <property type="entry name" value="Arg_tRNA_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001412">
        <property type="entry name" value="aa-tRNA-synth_I_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001278">
        <property type="entry name" value="Arg-tRNA-ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005148">
        <property type="entry name" value="Arg-tRNA-synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036695">
        <property type="entry name" value="Arg-tRNA-synth_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035684">
        <property type="entry name" value="ArgRS_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008909">
        <property type="entry name" value="DALR_anticod-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009080">
        <property type="entry name" value="tRNAsynth_Ia_anticodon-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11956">
        <property type="entry name" value="PTHR11956"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03485">
        <property type="entry name" value="Arg_tRNA_synt_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05746">
        <property type="entry name" value="DALR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00750">
        <property type="entry name" value="tRNA-synt_1d"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01038">
        <property type="entry name" value="TRNASYNTHARG"/>
    </dbReference>
    <dbReference type="SMART" id="SM01016">
        <property type="entry name" value="Arg_tRNA_synt_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00836">
        <property type="entry name" value="DALR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47323">
        <property type="entry name" value="SSF47323"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55190">
        <property type="entry name" value="SSF55190"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00456">
        <property type="entry name" value="argS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00178">
        <property type="entry name" value="AA_TRNA_LIGASE_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_1000018100" description="Arginine--tRNA ligase">
        <location>
            <begin position="1"/>
            <end position="602"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'HIGH' region">
        <location>
            <begin position="132"/>
            <end position="142"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00123"/>
        </source>
    </evidence>
    <sequence length="602" mass="65232" checksum="8B15650A3E944D6B" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-01-15" modified="2022-05-25" version="102" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LSF5</accession>
    <name>MINC_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Probable septum site-determining protein MinC</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">minC</name>
        <name type="ordered locus">Rmet_0035</name>
    </gene>
    <organism>
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Cell division inhibitor that blocks the formation of polar Z ring septums. Rapidly oscillates between the poles of the cell to destabilize FtsZ filaments that have formed before they mature into polar Z rings. Prevents FtsZ polymerization.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Interacts with MinD and FtsZ.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the MinC family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF06921.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011514963.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q1LSF5"/>
    <dbReference type="SMR" id="Q1LSF5"/>
    <dbReference type="STRING" id="266264.Rmet_0035"/>
    <dbReference type="EnsemblBacteria" id="ABF06921">
        <property type="protein sequence ID" value="ABF06921"/>
        <property type="gene ID" value="Rmet_0035"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0035"/>
    <dbReference type="eggNOG" id="COG0850">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_067812_0_0_4"/>
    <dbReference type="OrthoDB" id="1665744at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000902">
        <property type="term" value="P:cell morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000917">
        <property type="term" value="P:division septum assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051302">
        <property type="term" value="P:regulation of cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901891">
        <property type="term" value="P:regulation of cell septum assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00267">
        <property type="entry name" value="MinC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016098">
        <property type="entry name" value="CAP/MinC_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013033">
        <property type="entry name" value="MinC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036145">
        <property type="entry name" value="MinC_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007874">
        <property type="entry name" value="MinC_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005526">
        <property type="entry name" value="Septum_form_inhib_MinC_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34108">
        <property type="entry name" value="PTHR34108"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03775">
        <property type="entry name" value="MinC_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05209">
        <property type="entry name" value="MinC_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63848">
        <property type="entry name" value="SSF63848"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01222">
        <property type="entry name" value="minC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0131">Cell cycle</keyword>
    <keyword id="KW-0132">Cell division</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0717">Septation</keyword>
    <feature type="chain" id="PRO_1000047850" description="Probable septum site-determining protein MinC">
        <location>
            <begin position="1"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="103"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="105"/>
            <end position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="282" mass="29772" checksum="310E160AEF56F6A5" modified="2006-05-30" version="1">MSQKKSPRFELRSGNVDALLLALNTADLDAVRDDLLSRFESTPDFFSDDVVALDLRRLEGTGALALDRVIDTLATLKARAIGVVARADQRDWAGGFGLPLLDSQSRRGGKDEAPKEKAGKPEATAASGQTDAEAAGNTGKGKDSEGAAVNGKASEIAEIMAAANAASAPRAIPTLLIDKPLRSGQQIYAQGDVVILDLVSYGAEVIAEGNIHIYAPLRGRALAGVKGNPDARIFCTCLEPELISIAGIYRTAEQTLPADVLGKSAQVRLADEKLILEPLRMK</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-01-04" modified="2021-06-02" version="68" xmlns="http://uniprot.org/uniprot">
    <accession>Q5NUX9</accession>
    <name>Q5NUX9_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_6217</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="1 2">
        <name>pMOL28</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid pMOL28</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000355">
        <property type="protein sequence ID" value="ABF13076.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011239962.1">
        <property type="nucleotide sequence ID" value="NC_007972.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_161701.1">
        <property type="nucleotide sequence ID" value="NC_006525.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF13076">
        <property type="protein sequence ID" value="ABF13076"/>
        <property type="gene ID" value="Rmet_6217"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6217"/>
    <dbReference type="HOGENOM" id="CLU_2421977_0_0_4"/>
    <dbReference type="OMA" id="ACFGHEL"/>
    <dbReference type="OrthoDB" id="2043056at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid pMOL28"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF13076.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="94" mass="10673" checksum="15778F11D656D969" modified="2005-01-04" version="1">MSKVFVTAEVAEKLLPRRRKVHTFIRIFGWQGADVDREKLLEVFHAAKSVEVSQDAACFDHYLAVKIDGMVTYVETNLKALAKFGLLPPNRKLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-03-23" modified="2020-10-07" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>D3DXM4</accession>
    <name>D3DXM4_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Putative chromate transport protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_6427</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ADC45044.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_6427"/>
    <dbReference type="EnsemblBacteria" id="ADC45044">
        <property type="protein sequence ID" value="ADC45044"/>
        <property type="gene ID" value="Rmet_6427"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6427"/>
    <dbReference type="HOGENOM" id="CLU_3398081_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADC45044.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="31" mass="3482" checksum="18942D61752D0625" modified="2010-03-23" version="1">MLFMLLLTLVLYSETHISVLVSGALTSVWPF</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-03-23" modified="2020-10-07" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>D3DXS9</accession>
    <name>D3DXS9_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_6491</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ADC45099.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_6491"/>
    <dbReference type="EnsemblBacteria" id="ADC45099">
        <property type="protein sequence ID" value="ADC45099"/>
        <property type="gene ID" value="Rmet_6491"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6491"/>
    <dbReference type="HOGENOM" id="CLU_2882718_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADC45099.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="63" mass="7116" checksum="3D7CB919333316AD" modified="2010-03-23" version="1">MWADSPVLWVNWLLDARTLYSDPSETSDSFEVAAILANRGHKTLPSAITRIGYRPDVLQKLTQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-03-23" modified="2020-10-07" version="51" xmlns="http://uniprot.org/uniprot">
    <accession>D3DXY5</accession>
    <name>D3DXY5_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Putative sensory box protein/GGDEF family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Rmet_6548</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ADC45155.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_6548"/>
    <dbReference type="EnsemblBacteria" id="ADC45155">
        <property type="protein sequence ID" value="ADC45155"/>
        <property type="gene ID" value="Rmet_6548"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6548"/>
    <dbReference type="eggNOG" id="COG2202">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_597002_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00130">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001610">
        <property type="entry name" value="PAC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000014">
        <property type="entry name" value="PAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000700">
        <property type="entry name" value="PAS-assoc_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035965">
        <property type="entry name" value="PAS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013655">
        <property type="entry name" value="PAS_fold_3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08447">
        <property type="entry name" value="PAS_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00086">
        <property type="entry name" value="PAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00091">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55785">
        <property type="entry name" value="SSF55785"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00229">
        <property type="entry name" value="sensory_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50113">
        <property type="entry name" value="PAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50112">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="18"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="210"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="domain" description="PAS" evidence="2">
        <location>
            <begin position="247"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="domain" description="PAC" evidence="3">
        <location>
            <begin position="322"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="domain" description="PAS" evidence="2">
        <location>
            <begin position="375"/>
            <end position="419"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ADC45155.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="458" mass="50840" checksum="CA9F016D7708F608" modified="2010-03-23" version="1">MRRAGEGSLPAGEQVSSLIWPFVPVVVALALFCIASVDVLSAARSFVAGESLWTKGQKAAVLHLLNYSRSQLDTDYDAYLAAIAVPLGDRQARIELEKHSRYDHTRAVEGLLIGGNHTDDISGMIRLYRTFRGVAEIDEAIEIWAAADVDIARLDTLSRTLRGRFRAGDCDARCVAPIRREIVDIDNRLTPQEAAFSSTLGRASRRVRALLTWSSIAVAVTLLAWVTWVSRSMMIKERQLRAALSTSEERLQLAVAGSNDGLWDWHPHRGELYFSPRCAEMLGYRANELPHAVETLMALTHPDDVTELGRRLSGHMRKGTPYDIELRLRKKAGGYLWTRARGRMVRSAVGKAVRMAGSLSDISDRKRFEAQLHTEKERAQVTLQAIGEAVITTDVWGRIDSLNPVAEAMTGWTEAEAAGCFLPQVCPLTDEVTCDLVTDLVPRALRWPGRGRARLSRC</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-03-23" modified="2020-10-07" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>D3DY90</accession>
    <name>D3DY90_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_6662</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ADC45260.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_6662"/>
    <dbReference type="EnsemblBacteria" id="ADC45260">
        <property type="protein sequence ID" value="ADC45260"/>
        <property type="gene ID" value="Rmet_6662"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6662"/>
    <dbReference type="HOGENOM" id="CLU_2719422_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADC45260.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="72" mass="7741" checksum="9FC7763B5834D2B3" modified="2010-03-23" version="1">MAGLGAAIAAVVHAAGILPRRFSGVIGSPFPKDFLLCLSCFRSTSVQLRVCSRAVAYRNCGLTLRKTMITIW</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-03-23" modified="2020-10-07" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>D3DYE2</accession>
    <name>D3DYE2_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_6733</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ADC45312.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_6733"/>
    <dbReference type="EnsemblBacteria" id="ADC45312">
        <property type="protein sequence ID" value="ADC45312"/>
        <property type="gene ID" value="Rmet_6733"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6733"/>
    <dbReference type="HOGENOM" id="CLU_2846695_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADC45312.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="65" mass="7208" checksum="EDEFCADB6CA579F9" modified="2010-03-23" version="1">MYIGSTINRIVRGAQGAWHAPWREFAQQSGNDFHDNPTFVAEPGEVLLPLGGSPVLRHSRRAKPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-03-23" modified="2020-10-07" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>D3DYF3</accession>
    <name>D3DYF3_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_6744</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ADC45323.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_6744"/>
    <dbReference type="EnsemblBacteria" id="ADC45323">
        <property type="protein sequence ID" value="ADC45323"/>
        <property type="gene ID" value="Rmet_6744"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6744"/>
    <dbReference type="HOGENOM" id="CLU_2495635_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADC45323.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="86" mass="9698" checksum="9296F84FD043B635" modified="2010-03-23" version="1">MTHKWPSHYRIAPRVSGSDCAGFPQRGMLASASALQFLGVDQRVHKLAALPTILVGPLWYEHYRRYVQGSVPREFPVSQTRALYAT</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1994-10-01" modified="2022-05-25" version="101" xmlns="http://uniprot.org/uniprot">
    <accession>P37975</accession>
    <accession>Q5NUX1</accession>
    <accession>Q7B058</accession>
    <name>CNRR_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName>Nickel and cobalt resistance protein CnrR</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">cnrR</name>
        <name type="synonym">cnrX</name>
        <name type="ordered locus">Rmet_6206</name>
        <name type="ORF">RMe0087</name>
    </gene>
    <organism>
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid">
        <name>pMOL28</name>
    </geneLocation>
    <reference key="1">
        <citation type="journal article" date="1993" name="J. Bacteriol." volume="175" first="767" last="778">
            <title>Characterization of the inducible nickel and cobalt resistance determinant cnr from pMOL28 of Alcaligenes eutrophus CH34.</title>
            <authorList>
                <person name="Liesegang H."/>
                <person name="Lemke K."/>
                <person name="Siddiqui R.A."/>
                <person name="Schlegel H.G."/>
            </authorList>
            <dbReference type="PubMed" id="8380802"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC DNA]</scope>
    </reference>
    <reference key="2">
        <citation type="submission" date="2004-10" db="EMBL/GenBank/DDBJ databases">
            <title>Sequence and features of the Ralstonia metallidurans CH34 heavy metal plasmids pMOL28 and pMOL30.</title>
            <authorList>
                <person name="van der Lelie D."/>
                <person name="Monchy S."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="Benotmane M."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Mergeay M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference key="3">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <reference key="4">
        <citation type="journal article" date="2000" name="J. Bacteriol." volume="182" first="1390" last="1398">
            <title>Regulation of the cnr cobalt and nickel resistance determinant from Ralstonia sp. strain CH34.</title>
            <authorList>
                <person name="Grass G."/>
                <person name="Grosse C."/>
                <person name="Nies D.H."/>
            </authorList>
            <dbReference type="PubMed" id="10671463"/>
            <dbReference type="DOI" id="10.1128/JB.182.5.1390-1398.2000"/>
        </citation>
        <scope>CHARACTERIZATION</scope>
        <scope>INDUCTION</scope>
        <scope>SUBCELLULAR LOCATION</scope>
    </reference>
    <reference key="5">
        <citation type="journal article" date="2000" name="J. Bacteriol." volume="182" first="1399" last="1409">
            <title>Regulation of the cnr cobalt and nickel resistance determinant of Ralstonia eutropha (Alcaligenes eutrophus) CH34.</title>
            <authorList>
                <person name="Tibazarwa C."/>
                <person name="Wuertz S."/>
                <person name="Mergeay M."/>
                <person name="Wyns L."/>
                <person name="van Der Lelie D."/>
            </authorList>
            <dbReference type="PubMed" id="10671464"/>
            <dbReference type="DOI" id="10.1128/JB.182.5.1399-1409.2000"/>
        </citation>
        <scope>SUBCELLULAR LOCATION</scope>
        <scope>INDUCTION</scope>
        <scope>SUGGESTION OF NICKEL BINDING</scope>
    </reference>
    <comment type="function">
        <text evidence="2 3">CnrH alone is able to activate cnr expression, while both CnrY and CrnR (CnrX) are needed for nickel induction of CnrH (PubMed:10671463). Has been suggested (PubMed:10671464) to bind nickel.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 3">Periplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="induction">
        <text evidence="2 3">By nickel and cobalt.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">To A.xylosoxydans NccX.</text>
    </comment>
    <dbReference type="EMBL" id="M91650">
        <property type="protein sequence ID" value="AAA21966.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AJ276513">
        <property type="protein sequence ID" value="CAB82449.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="X90708">
        <property type="protein sequence ID" value="CAI30231.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP000355">
        <property type="protein sequence ID" value="ABF13065.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C47056">
        <property type="entry name" value="C47056"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011239970.1">
        <property type="nucleotide sequence ID" value="NC_007972.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_161709.1">
        <property type="nucleotide sequence ID" value="NC_006525.1"/>
    </dbReference>
    <dbReference type="PDB" id="2Y39">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="1.41 A"/>
        <property type="chains" value="A=31-148"/>
    </dbReference>
    <dbReference type="PDB" id="2Y3B">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="1.55 A"/>
        <property type="chains" value="A=31-148"/>
    </dbReference>
    <dbReference type="PDB" id="2Y3D">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.30 A"/>
        <property type="chains" value="A/B=31-148"/>
    </dbReference>
    <dbReference type="PDB" id="2Y3G">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="1.91 A"/>
        <property type="chains" value="A/B/C/D=31-148"/>
    </dbReference>
    <dbReference type="PDB" id="2Y3H">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="1.89 A"/>
        <property type="chains" value="A/B/C/D=31-148"/>
    </dbReference>
    <dbReference type="PDB" id="3EPV">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="1.74 A"/>
        <property type="chains" value="A/B/C/D=40-148"/>
    </dbReference>
    <dbReference type="PDB" id="3ZG1">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="1.85 A"/>
        <property type="chains" value="A/B/C/D=1-148"/>
    </dbReference>
    <dbReference type="PDB" id="4WWB">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="1.11 A"/>
        <property type="chains" value="A=31-148"/>
    </dbReference>
    <dbReference type="PDB" id="4WWD">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="1.30 A"/>
        <property type="chains" value="A=31-148"/>
    </dbReference>
    <dbReference type="PDB" id="4WWF">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="1.10 A"/>
        <property type="chains" value="A/B=31-148"/>
    </dbReference>
    <dbReference type="PDBsum" id="2Y39"/>
    <dbReference type="PDBsum" id="2Y3B"/>
    <dbReference type="PDBsum" id="2Y3D"/>
    <dbReference type="PDBsum" id="2Y3G"/>
    <dbReference type="PDBsum" id="2Y3H"/>
    <dbReference type="PDBsum" id="3EPV"/>
    <dbReference type="PDBsum" id="3ZG1"/>
    <dbReference type="PDBsum" id="4WWB"/>
    <dbReference type="PDBsum" id="4WWD"/>
    <dbReference type="PDBsum" id="4WWF"/>
    <dbReference type="AlphaFoldDB" id="P37975"/>
    <dbReference type="SMR" id="P37975"/>
    <dbReference type="EnsemblBacteria" id="ABF13065">
        <property type="protein sequence ID" value="ABF13065"/>
        <property type="gene ID" value="Rmet_6206"/>
    </dbReference>
    <dbReference type="GeneID" id="60825776"/>
    <dbReference type="KEGG" id="rme:Rmet_6206"/>
    <dbReference type="HOGENOM" id="CLU_147385_0_0_4"/>
    <dbReference type="OMA" id="QHLFAMR"/>
    <dbReference type="OrthoDB" id="1704773at2"/>
    <dbReference type="EvolutionaryTrace" id="P37975"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid pMOL28"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042597">
        <property type="term" value="C:periplasmic space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025961">
        <property type="entry name" value="Metal_resist"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13801">
        <property type="entry name" value="Metal_resist"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0170">Cobalt</keyword>
    <keyword id="KW-0175">Coiled coil</keyword>
    <keyword id="KW-0533">Nickel</keyword>
    <keyword id="KW-0574">Periplasm</keyword>
    <keyword id="KW-0614">Plasmid</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000089977" description="Nickel and cobalt resistance protein CnrR">
        <location>
            <begin position="27"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="topological domain" description="Periplasmic" evidence="1">
        <location>
            <begin position="28"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="54"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="sequence conflict" description="In Ref. 1; AAA21966/CAB82449." evidence="4" ref="1">
        <original>D</original>
        <variation>V</variation>
        <location>
            <position position="136"/>
        </location>
    </feature>
    <feature type="helix" evidence="5">
        <location>
            <begin position="41"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="helix" evidence="5">
        <location>
            <begin position="53"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="helix" evidence="5">
        <location>
            <begin position="96"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="helix" evidence="5">
        <location>
            <begin position="129"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="helix" evidence="5">
        <location>
            <begin position="132"/>
            <end position="144"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000269" key="2">
        <source>
            <dbReference type="PubMed" id="10671463"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="3">
        <source>
            <dbReference type="PubMed" id="10671464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="4"/>
    <evidence type="ECO:0007829" key="5">
        <source>
            <dbReference type="PDB" id="4WWF"/>
        </source>
    </evidence>
    <sequence length="148" mass="16633" checksum="1D1B57FE1FF6F36A" modified="2006-07-11" version="2" precursor="true">MMKSRTRRLSLSTLFGALLGVSVAAAWLYYSHRNEAGHGDLHEILHEAVPLDANEREILELKEDAFAQRRREIETRLRAANGKLADAIAKNPAWSPEVEAATQEVERAAGDLQRATLVHVFEMRAGLKPEHRPAYDRVLIDALRRGSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>Q1L9P9</accession>
    <name>Q1L9P9_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Endonuclease</fullName>
            <ecNumber evidence="6">3.1.30.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">Rmet_6268</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="10 11">
        <name>pMOL28</name>
    </geneLocation>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="11">Plasmid pMOL28</plasmid>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 6">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the DNA/RNA non-specific endonuclease family.</text>
    </comment>
    <dbReference type="EC" id="3.1.30.-" evidence="6"/>
    <dbReference type="EMBL" id="CP000355">
        <property type="protein sequence ID" value="ABF13127.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF13127">
        <property type="protein sequence ID" value="ABF13127"/>
        <property type="gene ID" value="Rmet_6268"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6268"/>
    <dbReference type="HOGENOM" id="CLU_055174_1_0_4"/>
    <dbReference type="OMA" id="NIGEWAY"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid pMOL28"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.570.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018524">
        <property type="entry name" value="DNA/RNA_endonuclease_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001604">
        <property type="entry name" value="DNA/RNA_non-sp_Endonuclease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044929">
        <property type="entry name" value="DNA/RNA_non-sp_Endonuclease_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020821">
        <property type="entry name" value="Extracellular_endonuc_su_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044925">
        <property type="entry name" value="His-Me_finger_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040255">
        <property type="entry name" value="Non-specific_endonuclease"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13966">
        <property type="entry name" value="PTHR13966"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01223">
        <property type="entry name" value="Endonuclease_NS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00892">
        <property type="entry name" value="Endonuclease_NS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00477">
        <property type="entry name" value="NUC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54060">
        <property type="entry name" value="SSF54060"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01070">
        <property type="entry name" value="NUCLEASE_NON_SPEC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6 10" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3 6" id="KW-0540">Nuclease</keyword>
    <keyword evidence="10" id="KW-0614">Plasmid</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004192986" description="Endonuclease" evidence="7">
        <location>
            <begin position="29"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="domain" description="Endonuclease_NS" evidence="9">
        <location>
            <begin position="56"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="domain" description="NUC" evidence="8">
        <location>
            <begin position="57"/>
            <end position="244"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU366055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ABF13127.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="304" mass="32465" checksum="7A64E3C901BDF197" modified="2006-05-30" version="1">MFNDALPAMIRQLTVALLLAVTAAAASAKDAAQCGNILEAGKPAASRPAANTKLLCYRAYAVLYSPQTRTALWSAERLTAAAADAARTLPRDSDFYEEDRLPAGERAQRRDFVRSGYDRGHLAPSGDFGDKASQAESFSLANVVPQNSTSNRRTWSHIETSTRKLARQYGAIHVVTGPAFTNAARMLNGRVRIPDYLWKAIYVPGVGAAAYVVRNDATQAYSVISISELSRFTGIDPFPALAPTKRGVALDLPPPTPHPGEKAARRVAFARLVGNDKAGDAAESLGGLVQHAGALATLMLAYAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="53" xmlns="http://uniprot.org/uniprot">
    <accession>Q1L9U6</accession>
    <name>Q1L9U6_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_6221</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2 3">
        <name>pMOL28</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid pMOL28</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000355">
        <property type="protein sequence ID" value="ABF13080.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF13080">
        <property type="protein sequence ID" value="ABF13080"/>
        <property type="gene ID" value="Rmet_6221"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6221"/>
    <dbReference type="HOGENOM" id="CLU_628025_0_0_4"/>
    <dbReference type="OMA" id="FAFNDWM"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid pMOL28"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043991">
        <property type="entry name" value="Gp3-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18897">
        <property type="entry name" value="Gp3-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="300"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="316"/>
            <end position="338"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF13080.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="417" mass="46128" checksum="4468BF6E9F15A24F" modified="2006-05-30" version="1">MRPPIIGKIRPGIKVLTKAARDKLEAVKIHDAGLARGDSFERIEGDITRATGIKTPLVPKNVPWFTCRASDFANPALAEDIVQRYGEDRNDGNGHRLWRFPVVFAFDDWLSNMPNQLVAWTKSGRQYFSEYGRDGRRYCKMYAPPVRDERANRAKRTWGGRTVILRQDNDIQDGLCDPQECPQYQGGHCNLSASFFFAIPHISGLGLIEMPTTSIYVLQKAHAAMQTVAMARGRLVGAKFWMSKQELEISRIDDTGKPVRQMQWLITLDAEIDIAALLDGSDRRPPALEMAEQAVSMLEGDREVRPDHGSVDDGTIEAPQTGTSESGVVQSAGSVPPSDTAGTEDLESEYLDLVKRLGLNSEDGARQLKAYAAATFGRGWSKREQDVRGFLAQLRTALTKPAAFREHVAAIAADVAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LAA5</accession>
    <name>Q1LAA5_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Plasmid replication-partition related protein ParB</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">parB</name>
        <name evidence="4" type="ordered locus">Rmet_6062</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4 5">
        <name>pMOL30</name>
    </geneLocation>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="5">Plasmid pMOL30</plasmid>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ParB family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000354">
        <property type="protein sequence ID" value="ABF12921.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011514773.1">
        <property type="nucleotide sequence ID" value="NC_007971.2"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF12921">
        <property type="protein sequence ID" value="ABF12921"/>
        <property type="gene ID" value="Rmet_6062"/>
    </dbReference>
    <dbReference type="GeneID" id="60825055"/>
    <dbReference type="KEGG" id="rme:Rmet_6062"/>
    <dbReference type="HOGENOM" id="CLU_023853_3_0_4"/>
    <dbReference type="OMA" id="RCLAYFK"/>
    <dbReference type="OrthoDB" id="653368at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid pMOL30"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004437">
        <property type="entry name" value="ParB/RepB/Spo0J"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003115">
        <property type="entry name" value="ParB/Sulfiredoxin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036086">
        <property type="entry name" value="ParB/Sulfiredoxin_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02195">
        <property type="entry name" value="ParBc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00470">
        <property type="entry name" value="ParB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110849">
        <property type="entry name" value="SSF110849"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00180">
        <property type="entry name" value="parB_part"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0614">Plasmid</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ParB" evidence="3">
        <location>
            <begin position="106"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="55"/>
            <end position="80"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00470"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF12921.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="369" mass="40140" checksum="FEFAFE0D298EEB67" modified="2006-05-30" version="1">MKQSRRLGHDAARPPAAEAPPQHIQGVVVPAEMPSANLNRPMSEILREQGILDEDPLAPPPPLQSTPAPAAAPTPSPSPRTAPVSGGASGVSPPPKALHIPIGSQQMLPMDQIDVSPHQPRLVFDQDALASLTSTIDLVGLNNPITVRRKDDGRFELIAGERRFRAFQLKHEAYIPGFVRDLGEKEAAILAMTDNDAREDLSDFERALGYQRLLKLGHCLNQMDISRCVGRSMATVSRCLAYFKLPQVVIDMLMQRPGLIGTKVVADFVAFHEQGCTELVIAAVVKVEKGMSQENALNWLKAEARKLTNPAAVVIPRRLQMSGKPVADAKIDGRNLILKCPKDVNPELLLRTIEKFLSEAAADDLDEAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="104" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LAU7</accession>
    <name>Q1LAU7_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Transcriptional regulator, GntR family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Rmet_5870</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="5">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="5">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12729.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518079.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5870"/>
    <dbReference type="EnsemblBacteria" id="ABF12729">
        <property type="protein sequence ID" value="ABF12729"/>
        <property type="gene ID" value="Rmet_5870"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5870"/>
    <dbReference type="eggNOG" id="COG2188">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_063236_2_2_4"/>
    <dbReference type="OMA" id="RGDRITM"/>
    <dbReference type="OrthoDB" id="1775418at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006744">
        <property type="term" value="P:ubiquinone biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd07377">
        <property type="entry name" value="WHTH_GntR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1410.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028978">
        <property type="entry name" value="Chorismate_lyase_/UTRA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000524">
        <property type="entry name" value="Tscrpt_reg_HTH_GntR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011663">
        <property type="entry name" value="UTRA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00392">
        <property type="entry name" value="GntR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07702">
        <property type="entry name" value="UTRA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00035">
        <property type="entry name" value="HTHGNTR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00345">
        <property type="entry name" value="HTH_GNTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00866">
        <property type="entry name" value="UTRA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64288">
        <property type="entry name" value="SSF64288"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50949">
        <property type="entry name" value="HTH_GNTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0456">Lyase</keyword>
    <keyword evidence="4" id="KW-0614">Plasmid</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0831">Ubiquinone biosynthesis</keyword>
    <feature type="domain" description="HTH gntR-type" evidence="3">
        <location>
            <begin position="6"/>
            <end position="73"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF12729.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="260" mass="29007" checksum="B5FA4B94501912AD" modified="2006-05-30" version="1">MSEAVVPLYHQIYVVLRQQIVEGRFGQGPLPGEIDLAKQFHASRVTMRRVFDRLVQEGLVRRHRGLGTFVNPHPVRPQVTEERATSLLGAIIDMGEKTAVKVISIDEVHATPEVAEALQIQVGDPVVKIVRVRHYRNRPLSHITVYLPIDLGRSLTRKDLENTPMLRLLEAAGVELGRASQVLTARLADVVVAPLLDVPVGGALLAVRRVVLDKNGRPVQLLMGQYRPDRYEYRMELSPMGGGDSANVWVENETRTGLRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="65" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LB64</accession>
    <name>Q1LB64_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_5753</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12612.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011520154.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5753"/>
    <dbReference type="EnsemblBacteria" id="ABF12612">
        <property type="protein sequence ID" value="ABF12612"/>
        <property type="gene ID" value="Rmet_5753"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5753"/>
    <dbReference type="eggNOG" id="ENOG5032SB1">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_142273_1_0_4"/>
    <dbReference type="OMA" id="CIALDMD"/>
    <dbReference type="OrthoDB" id="1836036at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="CDD" id="cd08026">
        <property type="entry name" value="DUF326"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044543">
        <property type="entry name" value="YHJQ-like"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF12612.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="116" mass="12841" checksum="95FC4C3A06A27F28" modified="2006-05-30" version="1">MIRPTVQENFSRYADCIAACNAAAAACLKCAAACLEEPDTRKMTRCIALDMDCAGIANLAASYMLRNSEFAPLVCEDCAEVCKWCKEECERYDHWHCQECAKACAACMEMCLKMTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LBC5</accession>
    <name>Q1LBC5_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_5692</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12551.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011520093.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5692"/>
    <dbReference type="EnsemblBacteria" id="ABF12551">
        <property type="protein sequence ID" value="ABF12551"/>
        <property type="gene ID" value="Rmet_5692"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5692"/>
    <dbReference type="eggNOG" id="COG0745">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_056502_0_0_4"/>
    <dbReference type="OMA" id="RRWMTPP"/>
    <dbReference type="OrthoDB" id="1456435at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF12551.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="406" mass="42746" checksum="9E58BC71A72A3DAD" modified="2006-05-30" version="1">MDSLITAAALALAAGDPLGALNRVALRDDPPALALRGIAMAQLGEFARAKALLRSAARAFGAKEAVSRARCIVAEAEVALASRDLGWRTKWLDAARVTLEAHGDHANAACARYLELRRQLLTGQLDAAEHALDAIDPTALPPALRVAHELVAAGVAMRRLRTQDARLALIRAEIFARDAGIPALLAEVQSATAQLDAPAARLIARGDIRSIGLDEVASLLASPTLVADACRLAVSKAGAVVSLASRPVLFALARTLAEAWPDDVARDTLIAHAFRTRHADETLRARLRVEIGRLRTVLSSMAGIHATKRGFALAPRQATEVAVLAQPVEDAHAMLLACLADGEAWSSSSLALALGASQRSIQRALETLAAAGKVQSIGRGRAQRWITAPMPGFTTTLLLHVPLPGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="114" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LBE5</accession>
    <name>Q1LBE5_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">DNA-binding response regulator in two-component regulatory system with CopS</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">copR2</name>
        <name evidence="6" type="ordered locus">Rmet_5672</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="7">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="7">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12531.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011520077.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5672"/>
    <dbReference type="EnsemblBacteria" id="ABF12531">
        <property type="protein sequence ID" value="ABF12531"/>
        <property type="gene ID" value="Rmet_5672"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5672"/>
    <dbReference type="eggNOG" id="COG0745">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_30_1_4"/>
    <dbReference type="OMA" id="WGGDTRV"/>
    <dbReference type="OrthoDB" id="1020672at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00383">
        <property type="entry name" value="trans_reg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001867">
        <property type="entry name" value="OmpR/PhoB-type_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006291">
        <property type="entry name" value="PcoR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016032">
        <property type="entry name" value="Sig_transdc_resp-reg_C-effctor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039420">
        <property type="entry name" value="WalR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48111">
        <property type="entry name" value="PTHR48111"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00486">
        <property type="entry name" value="Trans_reg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00862">
        <property type="entry name" value="Trans_reg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46894">
        <property type="entry name" value="SSF46894"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01387">
        <property type="entry name" value="cztR_silR_copR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51755">
        <property type="entry name" value="OMPR_PHOB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 3 6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="6" id="KW-0614">Plasmid</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Response regulatory" evidence="4">
        <location>
            <begin position="2"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="domain" description="OmpR/PhoB-type" evidence="5">
        <location>
            <begin position="125"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="OmpR/PhoB-type" evidence="3">
        <location>
            <begin position="125"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="2">
        <location>
            <position position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABF12531.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="228" mass="25518" checksum="23031F06FBF8E92F" modified="2006-05-30" version="1">MKLLVVEDETKTGEYLRQGLTEAGFIVDLVHSGLDGQHMALNESYDLLILDVMLPDIDGWRILQNIRAAGNPVPVLFLTARDSVADRVKGLELGADDYLVKPFAFSELLARVRTLLRRGAAQAPVDRIQIADLVLDLARRRATRAGRRIVLTGKEFALLELLARRRGEVLPRSLIASQVWDMNFDSDSNVIDVAIRRLRAKIDDDFDDKLIQTVRGMGYVLEAPEDLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="100" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LBH2</accession>
    <name>Q1LBH2_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Alcohol dehydrogenase, zinc-binding</fullName>
            <ecNumber evidence="4">1.1.1.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">adh</name>
        <name evidence="4" type="ordered locus">Rmet_5645</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="5">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="5">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the zinc-containing alcohol dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.-" evidence="4"/>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12504.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011520049.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5645"/>
    <dbReference type="EnsemblBacteria" id="ABF12504">
        <property type="protein sequence ID" value="ABF12504"/>
        <property type="gene ID" value="Rmet_5645"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5645"/>
    <dbReference type="eggNOG" id="COG1063">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026673_11_3_4"/>
    <dbReference type="OMA" id="CENCEKG"/>
    <dbReference type="OrthoDB" id="787596at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016616">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013149">
        <property type="entry name" value="ADH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013154">
        <property type="entry name" value="ADH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002328">
        <property type="entry name" value="ADH_Zn_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020843">
        <property type="entry name" value="PKS_ER"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08240">
        <property type="entry name" value="ADH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00107">
        <property type="entry name" value="ADH_zinc_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00829">
        <property type="entry name" value="PKS_ER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="SSF50129"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00059">
        <property type="entry name" value="ADH_ZINC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1 4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-0614">Plasmid</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="PKS_ER" evidence="3">
        <location>
            <begin position="11"/>
            <end position="351"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU361277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF12504.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="357" mass="37714" checksum="6897A8910B8E330F" modified="2006-05-30" version="1">MAGMQAAVFQGPGKIVLQEKPIPAVGPTDALIRITTTTICGTDIHILKGEYPVERGLTIGHEPVGIIEKLGSGVTGYHEGQRVIAGAICPSFYSYACQDGCASQDGQGHSHGYKPMGGWRFGNTIDGTQAEYVLVPDAQANLAPIPDGLTDEQVLMCPDIMSTGFSGAEHANIKIGDIVAVFAQGPIGLCATAGARLRGASLIIAVDGIDDRLNISRQMGADVTLNFRNTDVVQEILRITGGRGVDASIEALGTQATFESALRVLKPGGTLSSLGVYSSDLKIPLDAFAAGLGDHRIITSLCPGGKERMRRLMNVIETQRIDLGPLVTHRFKLADIEAAYDLFGHQRDGVLKVAITP</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LBI8</accession>
    <name>Q1LBI8_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Periplasmic tricarboxylate binding receptor (TctC)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">tctC</name>
        <name evidence="4" type="ordered locus">Rmet_5629</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="5">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="5">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Periplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the UPF0065 (bug) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12488.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008641542.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5629"/>
    <dbReference type="EnsemblBacteria" id="ABF12488">
        <property type="protein sequence ID" value="ABF12488"/>
        <property type="gene ID" value="Rmet_5629"/>
    </dbReference>
    <dbReference type="GeneID" id="60824444"/>
    <dbReference type="KEGG" id="rme:Rmet_5629"/>
    <dbReference type="eggNOG" id="COG3181">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045683_1_0_4"/>
    <dbReference type="OMA" id="MLRGIFM"/>
    <dbReference type="OrthoDB" id="988124at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042597">
        <property type="term" value="C:periplasmic space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005064">
        <property type="entry name" value="BUG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042100">
        <property type="entry name" value="Bug_dom1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42928">
        <property type="entry name" value="PTHR42928"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03401">
        <property type="entry name" value="TctC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017082">
        <property type="entry name" value="YflP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-0675">Receptor</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193298" evidence="3">
        <location>
            <begin position="27"/>
            <end position="333"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF12488.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="333" mass="36607" checksum="F6D6C2B97B8F1AEB" modified="2006-05-30" version="1">MTLRYAARALACASLLGVAWASPAFAWEPTKPVEVVVPFSAGGASDQMARSIQGIIAKHKLMNQPMIVVNKAGASGAEGLMDTKASKGDAHKLLVTSSALYTVPMISQLPFNWRDLTPVAMVAMDEFVLWTNAEAPYKTPADYIAEAKKQPGKLKMGGTSSKREDQIITAQVERKAGVRFIYIPYKGGGEAATQLSGKHIDSNVNNPSESIGQWRAGEHRALCVFAPDRMQYTNKITGTQSWGDIPTCKEQGLDVQYQMLRVFMLPGGVTPDQQKYYVDLMQKVVATPEWKEYLEKNALKNDFLTGQKLTEFLTADETRHKDIIKEAGFLAAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LBK3</accession>
    <name>Q1LBK3_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">CDP-diacylglycerol pyrophosphatase</fullName>
            <ecNumber evidence="6">3.6.1.26</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">CDP-diacylglycerol phosphatidylhydrolase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="16">CDP-diglyceride hydrolase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="ordered locus">Rmet_5614</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="19">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="19" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="19">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a CDP-1,2-diacyl-sn-glycerol + H2O = a 1,2-diacyl-sn-glycero-3-phosphate + CMP + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:15221"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:58332"/>
            <dbReference type="ChEBI" id="CHEBI:58608"/>
            <dbReference type="ChEBI" id="CHEBI:60377"/>
            <dbReference type="EC" id="3.6.1.26"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="4">Lipid metabolism.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3">Phospholipid metabolism; CDP-diacylglycerol degradation; phosphatidate from CDP-diacylglycerol: step 1/1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the Cdh family.</text>
    </comment>
    <dbReference type="EC" id="3.6.1.26" evidence="6"/>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12473.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011520019.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5614"/>
    <dbReference type="EnsemblBacteria" id="ABF12473">
        <property type="protein sequence ID" value="ABF12473"/>
        <property type="gene ID" value="Rmet_5614"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5614"/>
    <dbReference type="eggNOG" id="COG2134">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_077117_0_0_4"/>
    <dbReference type="OMA" id="SPFIMLA"/>
    <dbReference type="OrthoDB" id="1854066at2"/>
    <dbReference type="UniPathway" id="UPA00219"/>
    <dbReference type="UniPathway" id="UPA00609">
        <property type="reaction ID" value="UER00664"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008715">
        <property type="term" value="F:CDP-diacylglycerol diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046342">
        <property type="term" value="P:CDP-diacylglycerol catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008654">
        <property type="term" value="P:phospholipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.428.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003763">
        <property type="entry name" value="CDP-diacylglyc_Pase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036265">
        <property type="entry name" value="HIT-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02611">
        <property type="entry name" value="CDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54197">
        <property type="entry name" value="SSF54197"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="13" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="14" id="KW-0472">Membrane</keyword>
    <keyword evidence="18" id="KW-0614">Plasmid</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="17" id="KW-0732">Signal</keyword>
    <keyword evidence="10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="17">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193280" description="CDP-diacylglycerol pyrophosphatase" evidence="17">
        <location>
            <begin position="20"/>
            <end position="256"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00006435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00019608"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00032888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00032892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="ABF12473.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="256" mass="27968" checksum="4911F7DC46985522" modified="2006-05-30" version="1">MKRLLLWCLALIASGQACADAGLLERMVGGFSRDSLWKNVQERCLTPATPTHSDCVIVDRQRGFVLYKDMIGASHYLVIPVRPVSGVDDARVWSDREHNAWAFGWAARDIVGKAIGRALPDTLLGLAINARAARSQDQLHIHLDCISESARDFLASGAFDRQWRSLTFRGKPVRVRLIPSDDPVLAVDPFDVVREDLGADGKIADRGVFLGYVRPLQGQAGFVVVDEPVDKAGGANGHASDFLDRGCKLGRAQPGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="64" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LBP2</accession>
    <name>Q1LBP2_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF1338 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_5575</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12434.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519984.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5575"/>
    <dbReference type="EnsemblBacteria" id="ABF12434">
        <property type="protein sequence ID" value="ABF12434"/>
        <property type="gene ID" value="Rmet_5575"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5575"/>
    <dbReference type="eggNOG" id="COG5383">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_818046_0_0_4"/>
    <dbReference type="OMA" id="IPWTYAY"/>
    <dbReference type="OrthoDB" id="714422at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009770">
        <property type="entry name" value="DUF1338"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07063">
        <property type="entry name" value="DUF1338"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01150">
        <property type="entry name" value="DUF1338"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF1338" evidence="1">
        <location>
            <begin position="44"/>
            <end position="336"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM01150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF12434.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="339" mass="37115" checksum="C6A315D0BF2996E5" modified="2006-05-30" version="1">MISNVTSLVSLSLGTEAAARLSQLVAMPTLPAGKLGHPTRAEIAHALNLVLFAGILDRVPTGKAYTEDVAATGGKVHFDHGALRTVRWQENGALPAGEAAFTRILRPLGYRLNGTYPLDRIGMTGRSYAHADAPEEIAQFFLSEFHPERYSEDFQQAVSRVVGNSADPLTPRAQSLLWELERDGALPLADAGELIDLLARCFERQHATPHLNDYERLLAESSEMAWIATEGNAFNHATDRVEDVFAVSDEQKRVGRPMKEKVEVSQSGRVKQTAFRADPVRRAFVGASGDEVVREVPGSFYEFITRDRYVDEAQAITRTDLGFDAGNAQGIFKMTAAAC</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LBQ6</accession>
    <name>Q1LBQ6_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transcriptional regulator (Lambda repressor-like DNA-binding domain)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_5561</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12420.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519967.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5561"/>
    <dbReference type="EnsemblBacteria" id="ABF12420">
        <property type="protein sequence ID" value="ABF12420"/>
        <property type="gene ID" value="Rmet_5561"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5561"/>
    <dbReference type="eggNOG" id="COG1396">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG3093">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007145_0_0_4"/>
    <dbReference type="OMA" id="PCPFDEL"/>
    <dbReference type="OrthoDB" id="34961at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00093">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001387">
        <property type="entry name" value="Cro/C1-type_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010982">
        <property type="entry name" value="Lambda_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="SMART" id="SM00530">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47413">
        <property type="entry name" value="SSF47413"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50943">
        <property type="entry name" value="HTH_CROC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH cro/C1-type" evidence="1">
        <location>
            <begin position="11"/>
            <end position="42"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF12420.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="1346" mass="149026" checksum="42CF379440B37C18" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LC28</accession>
    <name>Q1LC28_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Medium-chain-fatty-acid-CoA ligase (Medium-chain acyl-CoA synthetase)</fullName>
            <ecNumber evidence="3">6.2.1.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">alkK</name>
        <name evidence="3" type="ordered locus">Rmet_5439</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EC" id="6.2.1.-" evidence="3"/>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12298.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519845.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5439"/>
    <dbReference type="EnsemblBacteria" id="ABF12298">
        <property type="protein sequence ID" value="ABF12298"/>
        <property type="gene ID" value="Rmet_5439"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5439"/>
    <dbReference type="eggNOG" id="COG0318">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000022_59_5_4"/>
    <dbReference type="OMA" id="LWSHTMA"/>
    <dbReference type="OrthoDB" id="377638at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016874">
        <property type="term" value="F:ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025110">
        <property type="entry name" value="AMP-bd_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045851">
        <property type="entry name" value="AMP-bd_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020845">
        <property type="entry name" value="AMP-binding_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_Synth/Lig"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13193">
        <property type="entry name" value="AMP-binding_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00455">
        <property type="entry name" value="AMP_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0436">Ligase</keyword>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AMP-binding" evidence="1">
        <location>
            <begin position="17"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="domain" description="AMP-binding_C" evidence="2">
        <location>
            <begin position="453"/>
            <end position="527"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF12298.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="545" mass="60026" checksum="705BE94C691C3D20" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LC36</accession>
    <name>Q1LC36_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Enoyl-CoA hydratase/isomerase</fullName>
            <ecNumber evidence="5">4.2.1.17</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Rmet_5431</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="6">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="6">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Lipid metabolism; fatty acid beta-oxidation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 4">Belongs to the enoyl-CoA hydratase/isomerase family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.17" evidence="5"/>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12290.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5431"/>
    <dbReference type="EnsemblBacteria" id="ABF12290">
        <property type="protein sequence ID" value="ABF12290"/>
        <property type="gene ID" value="Rmet_5431"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5431"/>
    <dbReference type="eggNOG" id="COG1024">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009834_7_0_4"/>
    <dbReference type="OMA" id="QEAFTWW"/>
    <dbReference type="UniPathway" id="UPA00659"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004300">
        <property type="term" value="F:enoyl-CoA hydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016853">
        <property type="term" value="F:isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006635">
        <property type="term" value="P:fatty acid beta-oxidation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.12.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029045">
        <property type="entry name" value="ClpP/crotonase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045002">
        <property type="entry name" value="Ech1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018376">
        <property type="entry name" value="Enoyl-CoA_hyd/isom_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001753">
        <property type="entry name" value="Enoyl-CoA_hydra/iso"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014748">
        <property type="entry name" value="Enoyl-CoA_hydra_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43149">
        <property type="entry name" value="PTHR43149"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00378">
        <property type="entry name" value="ECH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52096">
        <property type="entry name" value="SSF52096"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00166">
        <property type="entry name" value="ENOYL_COA_HYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0413">Isomerase</keyword>
    <keyword evidence="5" id="KW-0456">Lyase</keyword>
    <keyword evidence="5" id="KW-0614">Plasmid</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABF12290.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="280" mass="30163" checksum="B37FFB7509B966F5" modified="2006-05-30" version="1">MLYQREALGMTADNSQRIVVTIEEGVAEVRLNRPDKMNALDQAMFDALVDTGRQLAEMPGLRAVVLSGEGKAFCAGLDMGRMASLSEGTDPSLKRGRLTPRTHGMSNHPQYACMVWRELPVPVIAAVHGAAFGGGLQVALGADLRYVTADAKLSIMEIKWGLVPDMGGMLLTRGLVRPDVLNELIYTGRIISGTEAVELGLATRVCEDPRVAAMAVAREIAMKSPDAIRAGKRLMRVTEDGDALAILQAESVEQDALIGAPNQTEAVRANMEKRAPRFLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="72" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LC46</accession>
    <name>Q1LC46_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">NLPA lipoprotein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Rmet_5421</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="7">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="7">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NlpA lipoprotein family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12280.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519828.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5421"/>
    <dbReference type="EnsemblBacteria" id="ABF12280">
        <property type="protein sequence ID" value="ABF12280"/>
        <property type="gene ID" value="Rmet_5421"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5421"/>
    <dbReference type="eggNOG" id="COG1464">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_067080_3_0_4"/>
    <dbReference type="OMA" id="QPARDSI"/>
    <dbReference type="OrthoDB" id="1230456at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004872">
        <property type="entry name" value="Lipoprotein_NlpA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30429">
        <property type="entry name" value="PTHR30429"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03180">
        <property type="entry name" value="Lipoprotein_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0614">Plasmid</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004192994" evidence="5">
        <location>
            <begin position="30"/>
            <end position="271"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008973"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABF12280.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="271" mass="28559" checksum="3DFEC9DFC360BD01" modified="2006-05-30" version="1">MAEKTYLHRRGVLTALLAMALPSPGSATAASGSVQPRYTVRIGVVAEPDSELVGAASDAALREGVRLETVRFASVAQANAAVTTGRIEANIAQDGPSLARAGSGLLPVAHTVTYPMGLYSRTLSDLRGLPPRSVVAFPAEPSAQGRALLLLQGYGLIRISSDAGLSPRTTDIVENRRGLRWRAVAEARLAAALDNAALVALPFAAASAAGLAPARDALGMEDSRVPFANVLAIRARDRDAPWLTPTVRGLHSEPVKTFILARFNDSVRRPW</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="62" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LC73</accession>
    <name>Q1LC73_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_5394</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12253.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519799.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5394"/>
    <dbReference type="EnsemblBacteria" id="ABF12253">
        <property type="protein sequence ID" value="ABF12253"/>
        <property type="gene ID" value="Rmet_5394"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5394"/>
    <dbReference type="eggNOG" id="COG1618">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_106681_1_0_4"/>
    <dbReference type="OMA" id="WCRTACA"/>
    <dbReference type="OrthoDB" id="1798498at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018912">
        <property type="entry name" value="DUF2478"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10649">
        <property type="entry name" value="DUF2478"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF12253.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="191" mass="20307" checksum="6E08E926B49DB62A" modified="2006-05-30" version="1">MPQDSHRGSKPDHAGDSTAQVLPIAAIVHADHAGTDRLLTKFVDGLRHAGLRIHGLVQERAENGDKTSTMLVDQHSGQRYPLFQNLGSGSTSCCVDAGSIAAASVAMRRAVEARPDLCVANRFGGLEAQGGGLAAEMLNVAAAGIPLLTIFSNDYLRAWRAFTGDSGTELQPSIEAMYLWFAGLRARHARS</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LCH5</accession>
    <name>Q1LCH5_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Taurine dioxygenase</fullName>
            <ecNumber evidence="3">1.14.11.17</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">tauD</name>
        <name evidence="3" type="ordered locus">Rmet_5292</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EC" id="1.14.11.17" evidence="3"/>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12151.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519696.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5292"/>
    <dbReference type="EnsemblBacteria" id="ABF12151">
        <property type="protein sequence ID" value="ABF12151"/>
        <property type="gene ID" value="Rmet_5292"/>
    </dbReference>
    <dbReference type="GeneID" id="60826507"/>
    <dbReference type="KEGG" id="rme:Rmet_5292"/>
    <dbReference type="eggNOG" id="COG2175">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036005_2_1_4"/>
    <dbReference type="OMA" id="RHFGKPN"/>
    <dbReference type="OrthoDB" id="1742732at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000908">
        <property type="term" value="F:taurine dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.130.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042098">
        <property type="entry name" value="TauD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003819">
        <property type="entry name" value="TauD/TfdA-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02668">
        <property type="entry name" value="TauD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="1 3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TauD" evidence="2">
        <location>
            <begin position="3"/>
            <end position="273"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF12151.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="281" mass="31354" checksum="3B68FD58DB65C31F" modified="2006-05-30" version="1">MKIRQITPAIGAEISGVNIGEAARDPAVFAEIRAALLKHRVLFFRKQEITRADHVAFASGFGKLEDHPVVGSVPDYPGLVKVYRSDNPHSFENSYHCDGLWRPNPAMGAVLRCLECPEIGGDTIWVNMVKAYEELPDEIKSKIDGLRARASIEHSFGAVMTPENRAKLAQDHPPVEHPVVRIHPETGEKILFVGASFTTHFTNYSTPANVRHGIDKSPGASLLLNYLTSRATIPEYQVRWAWQEGDVAVWDNRSTQHYAVNDYYPAPRKMERAGIVGDIPY</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="92" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LCK5</accession>
    <name>Q1LCK5_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Flagellar motor switch protein FliG</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">fliG</name>
        <name evidence="13" type="ordered locus">Rmet_5262</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="14">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="14">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Bacterial flagellum basal body</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
            <orientation evidence="2">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the FliG family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12121.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519667.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5262"/>
    <dbReference type="EnsemblBacteria" id="ABF12121">
        <property type="protein sequence ID" value="ABF12121"/>
        <property type="gene ID" value="Rmet_5262"/>
    </dbReference>
    <dbReference type="GeneID" id="60826537"/>
    <dbReference type="KEGG" id="rme:Rmet_5262"/>
    <dbReference type="eggNOG" id="COG1536">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047835_2_0_4"/>
    <dbReference type="OMA" id="QVRPFEF"/>
    <dbReference type="OrthoDB" id="847822at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009425">
        <property type="term" value="C:bacterial-type flagellum basal body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003774">
        <property type="term" value="F:cytoskeletal motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071973">
        <property type="term" value="P:bacterial-type flagellum-dependent cell motility"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006935">
        <property type="term" value="P:chemotaxis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000090">
        <property type="entry name" value="Flg_Motor_Flig"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023087">
        <property type="entry name" value="Flg_Motor_Flig_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011002">
        <property type="entry name" value="FliG_a-hlx"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032779">
        <property type="entry name" value="FliG_M"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028263">
        <property type="entry name" value="FliG_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30534">
        <property type="entry name" value="PTHR30534"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01706">
        <property type="entry name" value="FliG_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14841">
        <property type="entry name" value="FliG_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14842">
        <property type="entry name" value="FliG_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF003161">
        <property type="entry name" value="FliG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00954">
        <property type="entry name" value="FLGMOTORFLIG"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48029">
        <property type="entry name" value="SSF48029"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00207">
        <property type="entry name" value="fliG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0975">Bacterial flagellum</keyword>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="13" id="KW-0966">Cell projection</keyword>
    <keyword evidence="6" id="KW-0145">Chemotaxis</keyword>
    <keyword evidence="13" id="KW-0969">Cilium</keyword>
    <keyword evidence="7" id="KW-0283">Flagellar rotation</keyword>
    <keyword evidence="13" id="KW-0282">Flagellum</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-0614">Plasmid</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FliG_N" evidence="12">
        <location>
            <begin position="12"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="domain" description="FliG_M" evidence="11">
        <location>
            <begin position="122"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="domain" description="FliG_C" evidence="10">
        <location>
            <begin position="224"/>
            <end position="329"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004287"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010299"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00021870"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022500"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022779"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023143"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF01706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF14841"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF14842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ABF12121.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="337" mass="37047" checksum="6F2FA6DA36BEF065" modified="2006-05-30" version="1">MTKANKGVPEKGLQKSAILMMAVGEDAAAEVFKHLSPREVQQLGSAMANLSSVTREEMSEVLSEFRNETEQYLALDVDSSTFIKSVLNKALGEERALSVIEDILESRDPGGGIDSLNWMDSTSVAELIKDEHPQIIATILIHLDRQKSSEVLGFFTERLRNDVILRIATFGGVQPGALQELTDVLTKLLAGQSLKRSRMGGVRTAAEIINLMSTAHEEAVIDGVRTHDADLAQKIIDEMFLFENLLEIEDRGIQRVLKEVATESLIVALKGAPQELRDKFIRNMSTRAGEMLREDLEALGPVRVSQVEAEQKAILQVVRRLAEAGEIQIGGGDDAYV</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="64" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LCL0</accession>
    <name>Q1LCL0_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Flg_hook domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_5257</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12116.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519662.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5257"/>
    <dbReference type="EnsemblBacteria" id="ABF12116">
        <property type="protein sequence ID" value="ABF12116"/>
        <property type="gene ID" value="Rmet_5257"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5257"/>
    <dbReference type="eggNOG" id="COG3144">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033226_1_0_4"/>
    <dbReference type="OMA" id="PGTPMEW"/>
    <dbReference type="OrthoDB" id="1388668at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021136">
        <property type="entry name" value="Flagellar_hook_control-like_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02120">
        <property type="entry name" value="Flg_hook"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Flg_hook" evidence="2">
        <location>
            <begin position="364"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="37"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="211"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="37"/>
            <end position="52"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF12116.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="447" mass="45918" checksum="1F91675005E2CBE7" modified="2006-05-30" version="1">MSIPIHSQPDAGQLARNDLLTQRTALAVDKLAALMPVQDTNETSSRDTSTDLGTAGRVPGARPGEASAGNTAGAVVTSTRETLSFAARTILDLFANADEAAAALKSTTPLATRTEMQSAPLLATKLASLLDQSGMFYEAHLAEWVSGARTLKDIAREPQAVLARATANGANPPAPDEPLRQLLALPYSPRAADAARATMLAAVDLPDAPDGDATGRAGNTAHGAGTANGTGSAGGMAANRAAEVSSFGRHPPVTRTITVGDPIPAPDGARTQSLQAAQSYASVADAGNAPRASLLATHASSSEPPAAPPPAQGQIVHPATEGLMRQQLELLAAQQFRWQGEAWSGTQMSWEVSPNPVDEDGSGTQHQARSWTTRLVVQMPGMGMVEASVTLSPSMLDVRVNAPLDDTVARLSAAIPDLHGRLAASGFVVGQITARADTAPTEAGVPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LCL1</accession>
    <name>Q1LCL1_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative flagellar protein FliT</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_5256</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12115.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519661.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5256"/>
    <dbReference type="EnsemblBacteria" id="ABF12115">
        <property type="protein sequence ID" value="ABF12115"/>
        <property type="gene ID" value="Rmet_5256"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5256"/>
    <dbReference type="eggNOG" id="ENOG5032ZWZ">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_155793_2_2_4"/>
    <dbReference type="OMA" id="VHLQEEY"/>
    <dbReference type="OrthoDB" id="2065730at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008622">
        <property type="entry name" value="FliT"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05400">
        <property type="entry name" value="FliT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0966">Cell projection</keyword>
    <keyword evidence="2" id="KW-0969">Cilium</keyword>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2" id="KW-0282">Flagellum</keyword>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF12115.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="114" mass="12941" checksum="5B357DC420EDB441" modified="2006-05-30" version="1">MYSKMPATPSVIQIYEELVLLSEQMLEAARHGNWDAMTEVQRVYVAQVETLRGRDTHPPATLQERMQRQALLAKLLAHDAAIRDLVMPQLQRLGELLGSARRRRDVNEAYGVSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="69" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LCQ6</accession>
    <name>Q1LCQ6_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">2-aminomuconate deaminase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">amnD</name>
        <name evidence="1" type="ordered locus">Rmet_5211</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12070.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008645543.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5211"/>
    <dbReference type="EnsemblBacteria" id="ABF12070">
        <property type="protein sequence ID" value="ABF12070"/>
        <property type="gene ID" value="Rmet_5211"/>
    </dbReference>
    <dbReference type="GeneID" id="60824889"/>
    <dbReference type="KEGG" id="rme:Rmet_5211"/>
    <dbReference type="eggNOG" id="COG0251">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_100715_7_3_4"/>
    <dbReference type="OMA" id="FLVNMND"/>
    <dbReference type="OrthoDB" id="1850770at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035959">
        <property type="entry name" value="RutC-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006175">
        <property type="entry name" value="YjgF/YER057c/UK114"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11803">
        <property type="entry name" value="PTHR11803"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01042">
        <property type="entry name" value="Ribonuc_L-PSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55298">
        <property type="entry name" value="SSF55298"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF12070.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="145" mass="15889" checksum="ACD8A153C922E042" modified="2006-05-30" version="1">MQANSASSVVAGKATPRGTFPHVRRAGDFLFVSGTSSRRADNTFEGVEVDAMGTTALDIRRQTRAVIENIRDILRSVGADLEDVVEITSFLVDMNDFGGYNAVYAEYFGYEGPTRTTVAVHQLPHPHLRIEIKAIAYRPLTSQAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LDD5</accession>
    <name>Q1LDD5_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Putative permease of the Major Facilitator Superfamily (Tn6048)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">mmfC2</name>
        <name evidence="2" type="ordered locus">Rmet_3003</name>
        <name evidence="3" type="ordered locus">Rmet_4038</name>
        <name evidence="4" type="ordered locus">Rmet_4979</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid</name>
    </geneLocation>
    <geneLocation type="plasmid" evidence="5">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="submission" date="2006-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of the chromosome of Ralstonia metallidurans CH34.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Copeland A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Barry K."/>
                <person name="Detter J.C."/>
                <person name="Glavina del Rio T."/>
                <person name="Hammon N."/>
                <person name="Israni S."/>
                <person name="Dalin E."/>
                <person name="Tice H."/>
                <person name="Martinez M."/>
                <person name="Goltsman E."/>
                <person name="Pitluck S."/>
                <person name="Schmutz J."/>
                <person name="Larimer F."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Kim E."/>
                <person name="Mergeay M."/>
                <person name="Benotmane M.A."/>
                <person name="Vallaeys T."/>
                <person name="Michaux A."/>
                <person name="Monchy S."/>
                <person name="Dunn J."/>
                <person name="McCorkle S."/>
                <person name="Taghavi S."/>
                <person name="van der Lelie D."/>
                <person name="Richardson P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">CH34</strain>
            <plasmid evidence="4">megaplasmid</plasmid>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="5">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2010-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Benotmane R."/>
                <person name="Lapidus A."/>
                <person name="McCorkle S."/>
                <person name="Monchy S."/>
                <person name="Taghavi S."/>
                <person name="van der Lelie N."/>
                <person name="Dunn J."/>
                <person name="Leys N."/>
                <person name="Mergeay M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">CH34</strain>
            <plasmid evidence="4">megaplasmid</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09876.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10906.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11841.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_005797954.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3003"/>
    <dbReference type="EnsemblBacteria" id="ABF09876">
        <property type="protein sequence ID" value="ABF09876"/>
        <property type="gene ID" value="Rmet_3003"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF10906">
        <property type="protein sequence ID" value="ABF10906"/>
        <property type="gene ID" value="Rmet_4038"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF11841">
        <property type="protein sequence ID" value="ABF11841"/>
        <property type="gene ID" value="Rmet_4979"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3003"/>
    <dbReference type="KEGG" id="rme:Rmet_4038"/>
    <dbReference type="KEGG" id="rme:Rmet_4979"/>
    <dbReference type="eggNOG" id="COG4705">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_070268_0_0_4"/>
    <dbReference type="OMA" id="WHRTLGS"/>
    <dbReference type="OrthoDB" id="1466669at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007136">
        <property type="entry name" value="DUF347"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03988">
        <property type="entry name" value="DUF347"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0614">Plasmid</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="43"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="72"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="98"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="163"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="192"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="224"/>
            <end position="243"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF09876.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF10906.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF11841.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="252" mass="26461" checksum="C075C2CC4B10BC14" modified="2006-05-30" version="1">MKTSTEHALAKVPEVTLGFWLIKIAATTLGETGGDAVSMSMNLGYLVGTAIFAAIFLAAVVAQVKAKGFHPFLYWTTIIATTTVGTTLADFADRSLGIGYAGGSSLLLALLLGSLFVWHRTLGSVSVSTVSSPKAEAFYWLTIMFSQTLGTALGDWTADTAGLGYTGAAVVFGGLLALVVAAYYWTSVSRTLLFWAAFILTRPLGAVVGDFLDKPLSAGGLALSRYSASAALLAFMLTAILLFRQRAARTAH</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LDE4</accession>
    <name>Q1LDE4_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Formyl-CoA transferase L-carnitine dehydratase/bile acid-inducible protein F</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_4970</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11832.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519391.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4970"/>
    <dbReference type="EnsemblBacteria" id="ABF11832">
        <property type="protein sequence ID" value="ABF11832"/>
        <property type="gene ID" value="Rmet_4970"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4970"/>
    <dbReference type="eggNOG" id="COG1804">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010587_0_1_4"/>
    <dbReference type="OMA" id="VENFTPR"/>
    <dbReference type="OrthoDB" id="969868at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1540.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10540">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003673">
        <property type="entry name" value="CoA-Trfase_fam_III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044855">
        <property type="entry name" value="CoA-Trfase_III_dom3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023606">
        <property type="entry name" value="CoA-Trfase_III_dom_1_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02515">
        <property type="entry name" value="CoA_transf_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89796">
        <property type="entry name" value="SSF89796"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF11832.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="823" mass="88383" checksum="7435FB78ACD0087B" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-10-07" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LDG3</accession>
    <name>Q1LDG3_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Extra-cytoplasmic solute receptor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">bugT</name>
        <name evidence="4" type="ordered locus">Rmet_4951</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="5">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="5">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Periplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the UPF0065 (bug) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11813.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4951"/>
    <dbReference type="EnsemblBacteria" id="ABF11813">
        <property type="protein sequence ID" value="ABF11813"/>
        <property type="gene ID" value="Rmet_4951"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4951"/>
    <dbReference type="eggNOG" id="COG3181">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045683_0_0_4"/>
    <dbReference type="OMA" id="AGHADFK"/>
    <dbReference type="OrthoDB" id="447734at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042597">
        <property type="term" value="C:periplasmic space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005064">
        <property type="entry name" value="BUG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042100">
        <property type="entry name" value="Bug_dom1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42928">
        <property type="entry name" value="PTHR42928"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03401">
        <property type="entry name" value="TctC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017082">
        <property type="entry name" value="YflP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-0675">Receptor</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193378" evidence="3">
        <location>
            <begin position="29"/>
            <end position="329"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF11813.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="329" mass="34754" checksum="71E4806D0EA3233D" modified="2006-05-30" version="1">MTLVSTLSRLGRMMAMVALFTAAAPTLAADNWPSRPIRFVVPFPAGGTADLIGRLISQQLAEILGQPVVVENRAGAGGNIGAALVASAAPDGYTLLQGTIGTHGINPTLYRHLNFDPARDFEPVARISSGTNVLVVNPNVPVKSVQELIALAKKEPGKLMMGSSGSGSSIHMSGELFQYMTGTRFTHVPYRGGAPAMTDLLGGRVQLMFDNSPTAIPHIQKGSLRPLAVTSAKRLPALPNVPTMIEAGVPDYVVSSWSGIFAPAGTPPAIVEKLNQAVATAFRSEKLRARYAEAGIEIEVMTVPEFRTFVQKEIARWATIVKASGASAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LDL8</accession>
    <name>Q1LDL8_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">AAA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_4896</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11758.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF11758">
        <property type="protein sequence ID" value="ABF11758"/>
        <property type="gene ID" value="Rmet_4896"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4896"/>
    <dbReference type="eggNOG" id="COG3910">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_079631_2_0_4"/>
    <dbReference type="OMA" id="MSHGESF"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140664">
        <property type="term" value="F:ATP-dependent DNA damage sensor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038729">
        <property type="entry name" value="Rad50/SbcC_AAA"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13304">
        <property type="entry name" value="AAA_21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13476">
        <property type="entry name" value="AAA_23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA" evidence="1">
        <location>
            <begin position="54"/>
            <end position="231"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF11758.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="264" mass="29499" checksum="3E0F32A44C40472F" modified="2006-05-30" version="1">MCKHCGRLNDFVAKHMLSSQFVSRVTLLRDKVPGFDRYPFSLPAVRELETLDLHPRVTFLVGENGSGKSTLLEGIAVALGFNAEGGSRNFSFATRASHSDLHEYLRIAKGVRRPSTGYFLRAESFYNVATEIERLDAEPGLGAPVINSYGGHSLHEQSHGEAFLTLLTERFQGKGLYILDEPEAALSPARQLAVISRLHDLVREGSQFVIATHSPILMAYPDACIYQCSARGIEPIAYQDTEHYQITRDFLVDPERMLRVLMED</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LDU4</accession>
    <name>Q1LDU4_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Assimilatory nitrate reductase (NADH) small subunit</fullName>
            <ecNumber evidence="6">1.7.1.4</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">nasB</name>
        <name evidence="6" type="ordered locus">Rmet_4820</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="7">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="7">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <dbReference type="EC" id="1.7.1.4" evidence="6"/>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11682.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519245.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4820"/>
    <dbReference type="EnsemblBacteria" id="ABF11682">
        <property type="protein sequence ID" value="ABF11682"/>
        <property type="gene ID" value="Rmet_4820"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4820"/>
    <dbReference type="eggNOG" id="COG1251">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003291_4_4_4"/>
    <dbReference type="OMA" id="YGLVEPA"/>
    <dbReference type="OrthoDB" id="1149616at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008942">
        <property type="term" value="F:nitrite reductase [NAD(P)H] activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.390.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023753">
        <property type="entry name" value="FAD/NAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016156">
        <property type="entry name" value="FAD/NAD-linked_Rdtase_dimer_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041575">
        <property type="entry name" value="Rubredoxin_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07992">
        <property type="entry name" value="Pyr_redox_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18267">
        <property type="entry name" value="Rubredoxin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="SSF51905"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0274">FAD</keyword>
    <keyword evidence="2" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-0614">Plasmid</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pyr_redox_2" evidence="4">
        <location>
            <begin position="12"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="domain" description="Rubredoxin_C" evidence="5">
        <location>
            <begin position="324"/>
            <end position="390"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF07992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF18267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABF11682.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="413" mass="44646" checksum="95FF6FBC90D5ADBF" modified="2006-05-30" version="1">MTPPVSSHAKPRLVVVGNGMAGMRTVEELLKLAPDLYDITVFGAEPHGNYNRILLSPVLAGEKTVDDIMLNTRAWYGQHGIELLAGDPVVAIDRPRRLVRSASGKEVRYDRLLLATGSKPFIIPVPGHTLDGVIAFRDIQDVETMLHAARNHRHAVVIGGGLLGLEAANGLMRQGMDVTVVHLADSLMERQLDKPAAELLKAALERKGLRFLLSAQTAAVLGSERVTGVLFKDGTQIPADLVVMTAGVRPNIELAASAGLRCERAIVVDDTLQTFDPRIYAVGECVQHRQATFGLVAPIWDQARVCAAHLAGAGHRRYVQQATATKLKVTGVDLYSAGDFIGGDNSEDLVLRDPRRGVYKRLVLQGGRLVGAVLYGDVQDGPWYFDLIQTGQPVAALRQRLLFGKAQCEALAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-10-07" version="65" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LE61</accession>
    <name>Q1LE61_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_4703</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11565.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4703"/>
    <dbReference type="EnsemblBacteria" id="ABF11565">
        <property type="protein sequence ID" value="ABF11565"/>
        <property type="gene ID" value="Rmet_4703"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4703"/>
    <dbReference type="eggNOG" id="COG2165">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_088953_0_1_4"/>
    <dbReference type="OMA" id="AIERYYL"/>
    <dbReference type="OrthoDB" id="2044924at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="164"/>
            <end position="203"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF11565.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="203" mass="22608" checksum="809240117B7D84C3" modified="2006-05-30" version="1">MSPMPSARLVRGCLRRRRGQGLVLLSLLIALLLMAIAMEGALDVWALQRQRQMEEELLFVGEQYRQAIQSYYLVGRALPASIDDLLEDKRFPVPLHHLRRAYPDPMTGKADWKLLIEGGRIYGVYSSSTAETIKRARFPRRYAEFANAKTYAEWQFFYVPPGRGIPGTNPGPQTPAPNTRGGKGAVPGNGNRTSPFAPLRPGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LEB5</accession>
    <name>Q1LEB5_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">RND efflux system, outer membrane lipoprotein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Rmet_4649</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="5">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="5">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the outer membrane factor (OMF) (TC 1.B.17) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11511.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519086.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4649"/>
    <dbReference type="EnsemblBacteria" id="ABF11511">
        <property type="protein sequence ID" value="ABF11511"/>
        <property type="gene ID" value="Rmet_4649"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4649"/>
    <dbReference type="eggNOG" id="COG1538">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012817_10_6_4"/>
    <dbReference type="OMA" id="NYEQSYA"/>
    <dbReference type="OrthoDB" id="1619477at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015562">
        <property type="term" value="F:efflux transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003423">
        <property type="entry name" value="OMP_efflux"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010131">
        <property type="entry name" value="RND_efflux_OM_lipoprot_NodT"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02321">
        <property type="entry name" value="OEP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01845">
        <property type="entry name" value="outer_NodT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2 4" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0564">Palmitate</keyword>
    <keyword evidence="4" id="KW-0614">Plasmid</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1134">Transmembrane beta strand</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5001442028" evidence="2">
        <location>
            <begin position="39"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="406"/>
            <end position="440"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU362097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF11511.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="495" mass="52805" checksum="768160394B8D4DAA" modified="2006-05-30" version="1">MASKALPGTHAQGLVKRTARAVALAAATLAALGMAACAVGPDYRTPDSEMPTQYQHAADVAAINDAREPVALDTWWAGFQDPVLTKIVERALEQNLDMAASIARVAQARAAANFAGAELLPQGNAYSDVTKLHQSVQSPLGKIGSTFPGYDRNQTLYSVGAAATWEIDIAGGLRRGAEAANAELQAAEANHMGVRILVAAEAADAYFRVRGAQARIAIAQQQVETNQRLLDLVRLRLADGIGAEREQAQAEAQLSQTRVTIPPLQIELETQLNRLDVLMGAQPGTYAAELRDSKHETQAVPQVALAQGPANLLERRPDVIAAERRLAASSARIGVATAEYYPKVSLSALLGFESLSAGKLFTAQAFQPAAAAGLRWRLFDFGRVNAEVAQAKGANAEALASYRKSMLQATEDVENALISLAQLESQNRELAVEIRAHERARNASEDAYKGGVVSLYEVLDEDRQLLAARDQQARVQADNARAAVSAFRALGGGWS</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="102" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LEB7</accession>
    <name>Q1LEB7_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Transcriptional regulator, LacI family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Rmet_4647</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="6">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="6">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11509.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519084.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4647"/>
    <dbReference type="EnsemblBacteria" id="ABF11509">
        <property type="protein sequence ID" value="ABF11509"/>
        <property type="gene ID" value="Rmet_4647"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4647"/>
    <dbReference type="eggNOG" id="COG1609">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037628_6_1_4"/>
    <dbReference type="OMA" id="CVAVDFR"/>
    <dbReference type="OrthoDB" id="1796334at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01392">
        <property type="entry name" value="HTH_LacI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000843">
        <property type="entry name" value="HTH_LacI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046335">
        <property type="entry name" value="LacI/GalR-like_sensor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010982">
        <property type="entry name" value="Lambda_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028082">
        <property type="entry name" value="Peripla_BP_I"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00356">
        <property type="entry name" value="LacI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13377">
        <property type="entry name" value="Peripla_BP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00354">
        <property type="entry name" value="HTH_LACI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47413">
        <property type="entry name" value="SSF47413"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53822">
        <property type="entry name" value="SSF53822"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50932">
        <property type="entry name" value="HTH_LACI_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0614">Plasmid</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH lacI-type" evidence="4">
        <location>
            <begin position="5"/>
            <end position="59"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABF11509.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="339" mass="36892" checksum="9E4AF3742A53C197" modified="2006-05-30" version="1">MTGKASVRDVAQAAGVSVGSVSRVLNGNGYASQELRGRVLAAVKALDYEPSFAARHLRTGRSRTVGYLTTDISNPLLAAHFSEVEQHMHAAGYSMLIGTTRNQHHRDRLLVELFETRRLEGIIASLSMESSDPAEDLFAKADLPIVIMDRETASPRDCVLIDHRTGLSLAVRYLKQLGHRRIAFFGPKESIRPGREKLLGYQEGLAEVGLLFDPALACTLPSMDSSRAAMKQMLALKQPPTAVIALGTRLLSGALYEARKAGLEVPHDLSVIALGTPETLELMYPPPTMLRFNTALSAKTAANLMLDRLEGHYAGEARRVVIPMDLMLGESCAPVRPGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LEC8</accession>
    <name>Q1LEC8_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Putative cytochrome c</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Rmet_4636</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="8">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="8">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11498.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519072.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4636"/>
    <dbReference type="EnsemblBacteria" id="ABF11498">
        <property type="protein sequence ID" value="ABF11498"/>
        <property type="gene ID" value="Rmet_4636"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4636"/>
    <dbReference type="eggNOG" id="COG2010">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_065353_0_0_4"/>
    <dbReference type="OMA" id="PFYLVWT"/>
    <dbReference type="OrthoDB" id="1469969at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.760.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009056">
        <property type="entry name" value="Cyt_c-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036909">
        <property type="entry name" value="Cyt_c-like_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00034">
        <property type="entry name" value="Cytochrom_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46626">
        <property type="entry name" value="SSF46626"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51007">
        <property type="entry name" value="CYTC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 4" id="KW-0349">Heme</keyword>
    <keyword evidence="3 4" id="KW-0408">Iron</keyword>
    <keyword evidence="2 4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0614">Plasmid</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193113" evidence="5">
        <location>
            <begin position="26"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="domain" description="Cytochrome c" evidence="6">
        <location>
            <begin position="197"/>
            <end position="285"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABF11498.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="290" mass="31412" checksum="5B43C326ECDE7B02" modified="2006-05-30" version="1">MPPWFAKLAGTLGCALMLLTGPAGAAGAADPATLTIVSGPQERSWTRQELLKDRRLVSVTVDDDNLKRRLTFKAIPLAALLHGLPVAADATATTAASDGYISHLPVRLLIADGADTPRAWLAVENPAAPWPKLKGQDIGPFRLIWTVPKGHAHNKISESSEINESLWTYSIVRIDVTVSPAERFAAIRPAADLPADSPVVRGFATFQRVCFSCHSMNRMGDATLGPDLNVPNSAVEYLGDEKLSALIRDPQSLRWWPGSRMSSIDQRTLSDADLKDLLAYLHHMAGRKAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-10-07" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LEG0</accession>
    <name>Q1LEG0_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_4604</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11466.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF11466">
        <property type="protein sequence ID" value="ABF11466"/>
        <property type="gene ID" value="Rmet_4604"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4604"/>
    <dbReference type="HOGENOM" id="CLU_2119074_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF11466.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="114" mass="12661" checksum="4FD24E6096D67008" modified="2006-05-30" version="1">MLVLALAPNTAVTFSRLGPIRVSRGSSRTSLSVRVIILPASLHVADIPRDWPTWVIWHEGSVIAPRNWMVPPHAGPAKEIEPIKASNKDSLVPCMITPERGSLFIHQRQPCWIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="58" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LEW8</accession>
    <name>Q1LEW8_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_4443</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11308.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518914.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4443"/>
    <dbReference type="EnsemblBacteria" id="ABF11308">
        <property type="protein sequence ID" value="ABF11308"/>
        <property type="gene ID" value="Rmet_4443"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4443"/>
    <dbReference type="eggNOG" id="COG0823">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_668760_0_0_4"/>
    <dbReference type="OMA" id="DETCFLH"/>
    <dbReference type="OrthoDB" id="821953at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF11308.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="431" mass="49253" checksum="629C84D73E2E8952" modified="2006-05-30" version="1">MHHDKIEHHPLPCRRLGDGELHHFFGYYNKSTWDRTDRYVLTQRVPMADADLHPGLIAEIGFFDTQDDDRFQKVGQTTAWNWQMGAQLQWLDGAPGRQLIYNCRTDDESAFYPCLGAVIHDVASGVSRPLPMPVYVVAPDSRYALCVDYRRLYVTHQTIGYSDEASLRALPQLDLAPASDGIHVMDLATGMSRLIVSYDALRNFHPVPSMDKAIHWVSHIEINPSSSRVLFLHRWTERVEDETCFLHRLITVNPDGTDMRLLECSDHPLPQLAADFDPNAVGTFDYEKSEYQISHPLWKDDTHIIVWGPHAGSIHYHLYEDRADGEVTVIGADVLTENGHMTFSRTHPDWLLSDTYPDAQTNERILFLYHVPSGVRHDIGSFYTSPTLKKENRCDLHPRWSRDGKRVCIDSIHDGNRQMYALDVASLVDPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LF84</accession>
    <name>Q1LF84_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Putative short chain dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_4327</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11192.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4327"/>
    <dbReference type="EnsemblBacteria" id="ABF11192">
        <property type="protein sequence ID" value="ABF11192"/>
        <property type="gene ID" value="Rmet_4327"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4327"/>
    <dbReference type="eggNOG" id="COG1028">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1833565_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF11192.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="140" mass="14231" checksum="6897E02F43B2773C" modified="2006-05-30" version="1">MPAQQQGTTTGRSGTVIGRSCEACSYRPVRRTILRSLAEGAAYKANAGASIVAALDGLARTLAVELAPIRVNVVSPGVVDSPTWDFLPSDARASVLHGIGAGLPVGRVGTASELAQAGRFLIANGFATGTVLQIDGGDVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="72" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LF95</accession>
    <name>Q1LF95_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Cupin 2, conserved barrel</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_4316</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11181.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518805.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4316"/>
    <dbReference type="EnsemblBacteria" id="ABF11181">
        <property type="protein sequence ID" value="ABF11181"/>
        <property type="gene ID" value="Rmet_4316"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4316"/>
    <dbReference type="eggNOG" id="COG1917">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_120069_1_2_4"/>
    <dbReference type="OMA" id="ELPGCHH"/>
    <dbReference type="OrthoDB" id="1928636at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013096">
        <property type="entry name" value="Cupin_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011051">
        <property type="entry name" value="RmlC_Cupin_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07883">
        <property type="entry name" value="Cupin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51182">
        <property type="entry name" value="SSF51182"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193092" evidence="1">
        <location>
            <begin position="33"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="domain" description="Cupin_2" evidence="2">
        <location>
            <begin position="61"/>
            <end position="129"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF11181.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="146" mass="15806" checksum="0C8426A08C9B880A" modified="2006-05-30" version="1">MTALAIRRNVLQVPLIAAMLLCTMAATRPALAHEAGHETTTPVMKQDIPEAKGRHVFMITVAYEPGQASEPHRHPGSIFAYVLDGEVESQLDGQPARVYKAGEAWYEPPGAKHLVSRNASSTKPAKLLVYGIAEGNMPVKLPLDAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LF97</accession>
    <name>Q1LF97_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">MFS efflux pump, membrane fusion protein, EmrA subfamily</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">emrA</name>
        <name evidence="6" type="ordered locus">Rmet_4314</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="7">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="7">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11179.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4314"/>
    <dbReference type="EnsemblBacteria" id="ABF11179">
        <property type="protein sequence ID" value="ABF11179"/>
        <property type="gene ID" value="Rmet_4314"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4314"/>
    <dbReference type="eggNOG" id="COG1566">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018816_15_0_4"/>
    <dbReference type="OMA" id="KGFASHA"/>
    <dbReference type="OrthoDB" id="1680841at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043602">
        <property type="entry name" value="CusB_dom_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032317">
        <property type="entry name" value="HlyD_D23"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00529">
        <property type="entry name" value="CusB_dom_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16576">
        <property type="entry name" value="HlyD_D23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0614">Plasmid</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="25"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="domain" description="HlyD_D23" evidence="5">
        <location>
            <begin position="62"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="domain" description="CusB_dom_1" evidence="4">
        <location>
            <begin position="292"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="349"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="386"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="389"/>
            <end position="413"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF16576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABF11179.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="423" mass="44920" checksum="1256FDC14A4E24AD" modified="2006-05-30" version="1">MTTTNPNPTDNPKAASGAPRTRKRLLALLGLGALVAAAGYGAYYYSVARFHEDTDDAYVNGNLVQLTPQVSGTVVAVNADDTEIVRAGDAIVTLDPADARIALANAEAALGQTVRQVSALYVNNNTLAATVQQRQVDLSRARDDLRRRTEASESGAVAAEDVAHARDTVKAAEAALETAKQQLASNHALTDQASVADHPSVQAAATRVRDAYLANARNTLPAPVTGYVARRSVQVGQRVAPGNPLMAIVPLDGVWVDANFKEVQLKHIRIGQPVELHADLYGGKVTYHGKVVGFSAGTGSAFSALPAQNATGNWIKVVQRLPVRIALDPVELRAHPLQVGLSMQVDVETRERGGELSPAKGTSTPIRTAYRTDVFERYGAEADSEIDRIIARNSNSNSDNSTRNETTRNARPVASPSALARAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="105" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LFG9</accession>
    <name>Q1LFG9_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Aconitate hydratase</fullName>
            <shortName evidence="12">Aconitase</shortName>
            <ecNumber evidence="12">4.2.1.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">acnA3</name>
        <name evidence="15" type="ordered locus">Rmet_4240</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="16">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="16">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Catalyzes the isomerization of citrate to isocitrate via cis-aconitate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11 12">
            <text>citrate = D-threo-isocitrate</text>
            <dbReference type="Rhea" id="RHEA:10336"/>
            <dbReference type="ChEBI" id="CHEBI:15562"/>
            <dbReference type="ChEBI" id="CHEBI:16947"/>
            <dbReference type="EC" id="4.2.1.3"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <text evidence="12">Binds 1 [4Fe-4S] cluster per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Carbohydrate metabolism; tricarboxylic acid cycle; isocitrate from oxaloacetate: step 2/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 12">Belongs to the aconitase/IPM isomerase family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.3" evidence="12"/>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11107.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4240"/>
    <dbReference type="EnsemblBacteria" id="ABF11107">
        <property type="protein sequence ID" value="ABF11107"/>
        <property type="gene ID" value="Rmet_4240"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4240"/>
    <dbReference type="eggNOG" id="COG1048">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013476_2_1_4"/>
    <dbReference type="OMA" id="ECPDSVV"/>
    <dbReference type="OrthoDB" id="363064at2"/>
    <dbReference type="UniPathway" id="UPA00223">
        <property type="reaction ID" value="UER00718"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003994">
        <property type="term" value="F:aconitate hydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047780">
        <property type="term" value="F:citrate dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009098">
        <property type="term" value="P:leucine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd01580">
        <property type="entry name" value="AcnA_IRP_Swivel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.19.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.499.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044137">
        <property type="entry name" value="AcnA_IRP_Swivel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015931">
        <property type="entry name" value="Acnase/IPM_dHydase_lsu_aba_1/3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001030">
        <property type="entry name" value="Acoase/IPM_deHydtase_lsu_aba"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015928">
        <property type="entry name" value="Aconitase/3IPM_dehydase_swvl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006249">
        <property type="entry name" value="Aconitase/IRP2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018136">
        <property type="entry name" value="Aconitase_4Fe-4S_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036008">
        <property type="entry name" value="Aconitase_4Fe-4S_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000573">
        <property type="entry name" value="AconitaseA/IPMdHydase_ssu_swvl"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11670">
        <property type="entry name" value="PTHR11670"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00330">
        <property type="entry name" value="Aconitase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00694">
        <property type="entry name" value="Aconitase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00415">
        <property type="entry name" value="ACONITASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53732">
        <property type="entry name" value="SSF53732"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01341">
        <property type="entry name" value="aconitase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00450">
        <property type="entry name" value="ACONITASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="5" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="10" id="KW-0100">Branched-chain amino acid biosynthesis</keyword>
    <keyword evidence="7 12" id="KW-0408">Iron</keyword>
    <keyword evidence="8 12" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="4" id="KW-0432">Leucine biosynthesis</keyword>
    <keyword evidence="9 12 15" id="KW-0456">Lyase</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-0614">Plasmid</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aconitase" evidence="13">
        <location>
            <begin position="84"/>
            <end position="553"/>
        </location>
    </feature>
    <feature type="domain" description="Aconitase_C" evidence="14">
        <location>
            <begin position="684"/>
            <end position="810"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004717"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU361275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="ABF11107.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="884" mass="94429" checksum="4EFD1669B4496000" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="96" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LFY2</accession>
    <name>Q1LFY2_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Putative short-chain dehydrogenase/reductase SDR</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_4076</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10944.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008644332.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4076"/>
    <dbReference type="EnsemblBacteria" id="ABF10944">
        <property type="protein sequence ID" value="ABF10944"/>
        <property type="gene ID" value="Rmet_4076"/>
    </dbReference>
    <dbReference type="GeneID" id="60823347"/>
    <dbReference type="KEGG" id="rme:Rmet_4076"/>
    <dbReference type="eggNOG" id="COG1028">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010194_1_2_4"/>
    <dbReference type="OMA" id="NMVVYVC"/>
    <dbReference type="OrthoDB" id="1209997at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF10944.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="264" mass="27562" checksum="578FC11F119814C3" modified="2006-05-30" version="1">MNIDLSGKTALVTGSTAGIGFATARGLAESGAHVIVNGRSQASVDNALASLKKSYPTAQATGFAGDLTDAAVCDTLVKTHPAVDILVNNLGIFEPKDFFDIPDSDWTRFFDVNVLSGVRLSRAYAKGMAERKWGRIVFIASESGINIPVEMIHYGFTKTAQLAVSRGLAKRMAGTGVTVNAVLPGPTLSEGVTAMLKDEIAKSGKSAEEVAAEFVKAHRSTSIIQRAASVEEVANMIVYVCSPQASATTGAALRVDGGVVDTIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LGJ3</accession>
    <name>Q1LGJ3_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Chromate transporter (CHR family)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">chrA2</name>
        <name evidence="8" type="ordered locus">Rmet_3865</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="9">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="9">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the chromate ion transporter (CHR) (TC 2.A.51) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10733.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518380.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3865"/>
    <dbReference type="EnsemblBacteria" id="ABF10733">
        <property type="protein sequence ID" value="ABF10733"/>
        <property type="gene ID" value="Rmet_3865"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3865"/>
    <dbReference type="eggNOG" id="COG2059">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018106_0_0_4"/>
    <dbReference type="OMA" id="WRAPPRC"/>
    <dbReference type="OrthoDB" id="1884249at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015109">
        <property type="term" value="F:chromate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014047">
        <property type="entry name" value="Chr_Tranpt_l_chain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003370">
        <property type="entry name" value="Chromate_transpt"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02417">
        <property type="entry name" value="Chromate_transp"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004810">
        <property type="entry name" value="ChrA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00937">
        <property type="entry name" value="2A51"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0614">Plasmid</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="15"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="83"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="119"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="148"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="193"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="222"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="257"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="285"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="319"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="346"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="372"/>
            <end position="388"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ABF10733.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="390" mass="41395" checksum="FB8C0A18A2807974" modified="2006-05-30" version="1">MNAPHDRPAYTLRQLVMYFVRLGALGFGGPVALAGHMRRDLVEARQWITDGDYKEGLALAQLAPGPLAAQLAIYLGYVHYRILGATLVGIAFVLPSFLMVVALGWAYVRFGGLTWMQSVFYGVGAAVIGIIAISAYKLTTRSVGKDKLLWAIYLLLCAVTVITESEVAWLFLASGVLVWLWRAPPKWLGHGRANAIAAIPAPAASALLGTIDWPLLSQIGLFFAKAGAFVFGSGLAIVPFLYGGVVTEYHWLNEKQFVDAVAVAMITPGPVVITVGFIGYLVAGLPGACVAAAGTFLPCYLFTILPAPYFKKYGKLPAILAFVDGVTAAAVGAISGAVIVLARRSIVDVPTVLLAVVTVALLLRFRKLPEPAIVAGAAALGLLIYPLLHH</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="92" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LGP0</accession>
    <name>Q1LGP0_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Transcriptional regulator, TetR family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Rmet_3816</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="5">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="5">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10686.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518332.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3816"/>
    <dbReference type="EnsemblBacteria" id="ABF10686">
        <property type="protein sequence ID" value="ABF10686"/>
        <property type="gene ID" value="Rmet_3816"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3816"/>
    <dbReference type="eggNOG" id="COG1309">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069356_46_3_4"/>
    <dbReference type="OMA" id="APREWMQ"/>
    <dbReference type="OrthoDB" id="1309950at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001647">
        <property type="entry name" value="HTH_TetR"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00440">
        <property type="entry name" value="TetR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50977">
        <property type="entry name" value="HTH_TETR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0614">Plasmid</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH tetR-type" evidence="3">
        <location>
            <begin position="31"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="H-T-H motif" evidence="1">
        <location>
            <begin position="54"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF10686.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="231" mass="25122" checksum="A1E17EC80B7026E6" modified="2006-05-30" version="1">MKAARPKAPTAPNPPSPSGARRRKPAQSRARATADAIRDAFFQLLAEKPYDRISIRQVIGLAGVGIGSFYEYFSCKDALAAVCIHLRVKAIAATMRASIDAHRTEPLPARVDALIDAQSAAPLAEPEHWAALFVVERKVSGIEAFRNMYAEFVQLWMETLSGGPEWPTDAPLGPTAFAVHAMTYSLVSQTLMTATRPVDAVALRRAVGNAVHGYLSIQAPLAYRMHRFEPG</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LGS7</accession>
    <name>Q1LGS7_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative monooxygenase, FAD/NAD(P)-binding domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_3779</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10649.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518295.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3779"/>
    <dbReference type="EnsemblBacteria" id="ABF10649">
        <property type="protein sequence ID" value="ABF10649"/>
        <property type="gene ID" value="Rmet_3779"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3779"/>
    <dbReference type="eggNOG" id="COG0654">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027335_1_0_4"/>
    <dbReference type="OMA" id="WRQFPVI"/>
    <dbReference type="OrthoDB" id="1033671at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002938">
        <property type="entry name" value="FAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01494">
        <property type="entry name" value="FAD_binding_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="SSF51905"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD_binding_3" evidence="1">
        <location>
            <begin position="111"/>
            <end position="321"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF10649.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="386" mass="42250" checksum="9AF513743E57642D" modified="2006-05-30" version="1">MKVAIIGGGPSGLFLSILLKERMAQVDIDVFEQNPEDATFGFGVVLADTGLSNLRAASPIVVDRLAKAMRFNDQHSIVSHEHPIVMRKPGAGGGAIPRISLLSVLQQRAKELGVRVTYNKRIADFDALDADLVVGADGIGSQLRTANEAAFGTTRRTLTNHFAWFGVAKPFASPALVFRKYEGGYFVAHYYPYSDSMSTFVAECDHQTWVDFGMEDMSAEARQALFETVFAPELTGHGLVSNNSIWRQFPVILNANWHVGKQVLIGDALTSAHFSIGSGTRIAMEDAMALADAIVTHPDDVPAALEQYEAVRKPEKAKLISASEASYNWYERIREWMDLPTPHEFVFRFMTRTGRIDAERLRAQFPALMAELAAGGVAAVAAEGQS</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2007-11-13" modified="2022-05-25" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LH07</accession>
    <name>Y3697_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">UPF0502 protein Rmet_3697</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">Rmet_3697</name>
    </gene>
    <organism>
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0502 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10569.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q1LH07"/>
    <dbReference type="SMR" id="Q1LH07"/>
    <dbReference type="STRING" id="266264.Rmet_3697"/>
    <dbReference type="EnsemblBacteria" id="ABF10569">
        <property type="protein sequence ID" value="ABF10569"/>
        <property type="gene ID" value="Rmet_3697"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3697"/>
    <dbReference type="eggNOG" id="COG3132">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_057831_0_0_4"/>
    <dbReference type="OMA" id="CNQKSSR"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01584">
        <property type="entry name" value="UPF0502"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007432">
        <property type="entry name" value="DUF480"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38768">
        <property type="entry name" value="PTHR38768"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04337">
        <property type="entry name" value="DUF480"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0614">Plasmid</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000309414" description="UPF0502 protein Rmet_3697">
        <location>
            <begin position="1"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01584"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="243" mass="26157" checksum="EF53C9FF0E074656" modified="2006-05-30" version="1">MTDTPDTPDTPMATGASSRPPLRALTPLEGRVVAVLLEKQFTVPDTYPLSLNALAAGCNQKTARSPVMSVGEDEILTAIDGLKSLSLVFEGSSSRVPRFEQNLARVLGVPSQSAALLSTLLLRGPQTAAELRLNTARLHAFADISSVEAFLDELAERDPALVVKLPRGPGEREHRWTHLLCGEVSLAEAPGLRTSASIEIAPSELEALRISHRELEDKVARLQALVEEMAEQLGISIDPDRLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="69" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LHG2</accession>
    <name>Q1LHG2_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Rmet_3542</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the porin LamB (TC 1.B.3) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10414.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3542"/>
    <dbReference type="EnsemblBacteria" id="ABF10414">
        <property type="protein sequence ID" value="ABF10414"/>
        <property type="gene ID" value="Rmet_3542"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3542"/>
    <dbReference type="eggNOG" id="COG4580">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_451805_0_0_4"/>
    <dbReference type="OMA" id="VQLNRRW"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046930">
        <property type="term" value="C:pore complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015288">
        <property type="term" value="F:porin activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034219">
        <property type="term" value="P:carbohydrate transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.170.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003192">
        <property type="entry name" value="Porin_LamB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036998">
        <property type="entry name" value="Porin_LamB_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02264">
        <property type="entry name" value="LamB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0406">Ion transport</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0626">Porin</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1134">Transmembrane beta strand</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="127"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="143"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="177"/>
            <end position="195"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABF10414.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="615" mass="63869" checksum="7CC720E1F56F20AD" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-04-14" modified="2022-05-25" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LHK6</accession>
    <name>ATPF_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">ATP synthase subunit b</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">ATP synthase F(0) sector subunit b</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">ATPase subunit I</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">F-type ATPase subunit b</fullName>
            <shortName evidence="1">F-ATPase subunit b</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">atpF</name>
        <name type="ordered locus">Rmet_3498</name>
    </gene>
    <organism>
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">F(1)F(0) ATP synthase produces ATP from ADP in the presence of a proton or sodium gradient. F-type ATPases consist of two structural domains, F(1) containing the extramembraneous catalytic core and F(0) containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation.</text>
    </comment>
    <comment type="function">
        <text evidence="1">Component of the F(0) channel, it forms part of the peripheral stalk, linking F(1) to F(0).</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">F-type ATPases have 2 components, F(1) - the catalytic core - and F(0) - the membrane proton channel. F(1) has five subunits: alpha(3), beta(3), gamma(1), delta(1), epsilon(1). F(0) has three main subunits: a(1), b(2) and c(10-14). The alpha and beta chains form an alternating ring which encloses part of the gamma chain. F(1) is attached to F(0) by a central stalk formed by the gamma and epsilon chains, while a peripheral stalk is formed by the delta and b chains.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell inner membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the ATPase B chain family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10370.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008650161.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q1LHK6"/>
    <dbReference type="SMR" id="Q1LHK6"/>
    <dbReference type="STRING" id="266264.Rmet_3498"/>
    <dbReference type="EnsemblBacteria" id="ABF10370">
        <property type="protein sequence ID" value="ABF10370"/>
        <property type="gene ID" value="Rmet_3498"/>
    </dbReference>
    <dbReference type="GeneID" id="60822604"/>
    <dbReference type="KEGG" id="rme:Rmet_3498"/>
    <dbReference type="eggNOG" id="COG0711">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_079215_4_5_4"/>
    <dbReference type="OMA" id="FAWKPIL"/>
    <dbReference type="OrthoDB" id="1999641at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045263">
        <property type="term" value="C:proton-transporting ATP synthase complex, coupling factor F(o)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046933">
        <property type="term" value="F:proton-transporting ATP synthase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01398">
        <property type="entry name" value="ATP_synth_b_bprime"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028987">
        <property type="entry name" value="ATP_synth_B-like_membr_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002146">
        <property type="entry name" value="ATP_synth_b/b'su_bac/chlpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005864">
        <property type="entry name" value="ATP_synth_F0_bsu_bac"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00430">
        <property type="entry name" value="ATP-synt_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81573">
        <property type="entry name" value="SSF81573"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01144">
        <property type="entry name" value="ATP_synt_b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0066">ATP synthesis</keyword>
    <keyword id="KW-0997">Cell inner membrane</keyword>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0138">CF(0)</keyword>
    <keyword id="KW-0375">Hydrogen ion transport</keyword>
    <keyword id="KW-0406">Ion transport</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <keyword id="KW-0813">Transport</keyword>
    <feature type="chain" id="PRO_0000368703" description="ATP synthase subunit b">
        <location>
            <begin position="1"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01398"/>
        </source>
    </evidence>
    <sequence length="156" mass="17273" checksum="63D0EFA8ABBCB4BC" modified="2006-05-30" version="1">MNLNATFFAQMVVFFILWWVVAKFIWPPLVKALDERAKKIADGLAAAEKGKAELELANKRVDQAMAEARTEGAQRVADAEKRAQLTADEIKQNAQAEAARIIAQAKAEAEQQVTRARESLRDQVAVLAVKGAEQILKREVNAQVHADLLNQLKAEL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-01-15" modified="2022-05-25" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LHS4</accession>
    <name>Y3430_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">UPF0102 protein Rmet_3430</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">Rmet_3430</name>
    </gene>
    <organism>
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0102 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10302.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q1LHS4"/>
    <dbReference type="SMR" id="Q1LHS4"/>
    <dbReference type="STRING" id="266264.Rmet_3430"/>
    <dbReference type="EnsemblBacteria" id="ABF10302">
        <property type="protein sequence ID" value="ABF10302"/>
        <property type="gene ID" value="Rmet_3430"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3430"/>
    <dbReference type="eggNOG" id="COG0792">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_115353_1_0_4"/>
    <dbReference type="OMA" id="RNWRCGR"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1350.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00048">
        <property type="entry name" value="UPF0102"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011335">
        <property type="entry name" value="Restrct_endonuc-II-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011856">
        <property type="entry name" value="tRNA_endonuc-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003509">
        <property type="entry name" value="UPF0102"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34039">
        <property type="entry name" value="PTHR34039"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02021">
        <property type="entry name" value="UPF0102"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52980">
        <property type="entry name" value="SSF52980"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00252">
        <property type="entry name" value="TIGR00252"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_1000009248" description="UPF0102 protein Rmet_3430">
        <location>
            <begin position="1"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00048"/>
        </source>
    </evidence>
    <sequence length="134" mass="14421" checksum="80A1A90BE51F662A" modified="2006-05-30" version="1">MPARPASSTTRQGALAEDRALAYLQRQGLVAVERNYRCKGGEIDLIMRAADDTLVFVEVRKRGGRGFGGAAASITLTKQRRVLRAASHYLATLDRLPPCRVDVVALDPGRLEWLRNAFDLGALDSEGGGEGPAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LHW8</accession>
    <name>Q1LHW8_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Type II secretion system protein I</fullName>
            <shortName evidence="5">T2SS minor pseudopilin I</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">gspI</name>
        <name evidence="7" type="ordered locus">Rmet_3386</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Component of the type II secretion system required for the energy-dependent secretion of extracellular factors such as proteases and toxins from the periplasm.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Type II secretion is composed of four main components: the outer membrane complex, the inner membrane complex, the cytoplasmic secretion ATPase and the periplasm-spanning pseudopilus.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cell inner membrane</location>
            <topology evidence="5">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="PTM">
        <text evidence="5">Cleaved by prepilin peptidase.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the GSP I family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10258.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517821.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3386"/>
    <dbReference type="EnsemblBacteria" id="ABF10258">
        <property type="protein sequence ID" value="ABF10258"/>
        <property type="gene ID" value="Rmet_3386"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3386"/>
    <dbReference type="eggNOG" id="COG2165">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_121289_2_1_4"/>
    <dbReference type="OMA" id="DCSQGNL"/>
    <dbReference type="OrthoDB" id="2071824at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015627">
        <property type="term" value="C:type II protein secretion system complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015628">
        <property type="term" value="P:protein secretion by the type II secretion system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012902">
        <property type="entry name" value="N_methyl_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045584">
        <property type="entry name" value="Pilin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003413">
        <property type="entry name" value="T2SS_GspI_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010052">
        <property type="entry name" value="T2SS_protein-GspI"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38779">
        <property type="entry name" value="PTHR38779"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07963">
        <property type="entry name" value="N_methyl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02501">
        <property type="entry name" value="T2SSI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54523">
        <property type="entry name" value="SSF54523"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01707">
        <property type="entry name" value="gspI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02532">
        <property type="entry name" value="IV_pilin_GFxxxE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00409">
        <property type="entry name" value="PROKAR_NTER_METHYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0488">Methylation</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="T2SSI" evidence="6">
        <location>
            <begin position="47"/>
            <end position="126"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008358"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU368030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF02501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABF10258.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="134" mass="14786" checksum="F04FACD3D3621B95" modified="2006-05-30" version="1">MSAPRLLQHRREGFTLIEVLVALTILAVALTAAMRAMGSMTESSISLQQRMLAEFSAENHLATLRLSKTWPEPGTRGYDCPQGGATLWCEESVTSTPNPSFRRVEISVFPSATNKSVRLAWLVTLVPNETRNVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LHX4</accession>
    <name>Q1LHX4_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Ca2+ sensor (EF-Hand superfamily)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Rmet_3380</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10252.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3380"/>
    <dbReference type="EnsemblBacteria" id="ABF10252">
        <property type="protein sequence ID" value="ABF10252"/>
        <property type="gene ID" value="Rmet_3380"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3380"/>
    <dbReference type="eggNOG" id="COG5126">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_091273_2_1_4"/>
    <dbReference type="OMA" id="GGGMWMK"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13202">
        <property type="entry name" value="EF-hand_5"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="SSF47473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193519" evidence="2">
        <location>
            <begin position="39"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="3">
        <location>
            <begin position="81"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="3">
        <location>
            <begin position="120"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="3">
        <location>
            <begin position="156"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="181"/>
            <end position="209"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF10252.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="209" mass="22775" checksum="C34FCEAC7D682D39" modified="2006-05-30" version="1">MPNVMKRSTDMQRNHKTHALKQFIAASAVFLVAGGAFAQTAAPAPSDGPQGMPMHRMGGPGEHRGGDWLMKLADTDKDGAISKAEFDALFNKIDTNHDGKLDKSELAAFHKAMWEQRRAEMKADFEAKFKAADKNNDGALTRDEFKAAFPRMANRFDRIDANHDGKVTMAEIEASMQKMHKERMDRMERHGPGGPGAAPAPAAPSSGGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="103" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LHX8</accession>
    <name>Q1LHX8_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Potassium:proton antiporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">kefC</name>
        <name evidence="8" type="ordered locus">Rmet_3376</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
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    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the monovalent cation:proton antiporter 2 (CPA2) transporter (TC 2.A.37) family.</text>
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        <property type="component" value="Chromosome"/>
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        <property type="term" value="C:integral component of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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        <property type="term" value="F:enzyme binding"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015503">
        <property type="term" value="F:glutathione-regulated potassium exporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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        <property type="project" value="InterPro"/>
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        <property type="term" value="F:toxic substance binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="P:response to methylglyoxal"/>
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        <property type="project" value="InterPro"/>
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        <property type="match status" value="1"/>
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    </dbReference>
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    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
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    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
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            <begin position="6"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
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            <end position="50"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
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    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="147"/>
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        </location>
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    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="187"/>
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        </location>
    </feature>
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    </feature>
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    </feature>
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        <location>
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            <end position="381"/>
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        <location>
            <begin position="406"/>
            <end position="528"/>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS51201"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="ABF10248.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="604" mass="65975" checksum="E223964FD8894D87" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="80" xmlns="http://uniprot.org/uniprot">
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        </submittedName>
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        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
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                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
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        <property type="taxonomic scope" value="Bacteria"/>
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    </evidence>
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        <source>
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    </evidence>
    <sequence length="493" mass="54137" checksum="72B7C3B6768BADEA" modified="2006-05-30" version="1">MKHAILWTCMFASFVANAETTIDPASIYSSHCAACHGADRLGGMGPALLPESLERLRPDDLLDVLRNGRPATQMQGFSKELGDATIKQMATWLRSAPAATPRWEQADIEASRIVYHAPGTLPARPVFSADPSNLFLVVEAGTHHVSVLDGDKLEPIHRFTTRFALHGGPKFSSDGRYVYMASRDGWVSKYDLWNLKLVAEVRAGMNTRNLAVSEDGEWVMVGNTLPQTLVLLRASDLSLQRIYPVTGAGGKSSRVSAVYDAAPRGSFIVALRDIPEVWEIPYIGKKTFEPRVIELTDVLDDFFFDQPYRHILGASRTATGQVIDLDAGRKVADLALTGMPHLGSGITWKRNGREVMASPNLKQGAISVIDMQSWKTVATIPTNGPGFFLRSHEATPYAWTDAMMSPKRDTLQVIDKQTLAIVGNVTPSPGRTAAHVEFTRDGRYALVSLMEADGAIVVYDARTLKEIKRLPMDKPIGKYNVFNKTTRSSGTSH</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LIX0</accession>
    <name>Q1LIX0_CUPMC</name>
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        <submittedName>
            <fullName evidence="4">Nuclease ParB</fullName>
        </submittedName>
    </protein>
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        <name evidence="4" type="ordered locus">Rmet_3033</name>
    </gene>
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        <name type="synonym">Ralstonia metallidurans</name>
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        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
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                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
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    </dbReference>
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    </dbReference>
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        <property type="gene ID" value="Rmet_3033"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3033"/>
    <dbReference type="eggNOG" id="COG1475">
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    </dbReference>
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    <dbReference type="OMA" id="REPYYIR"/>
    <dbReference type="OrthoDB" id="1375763at2"/>
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        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003115">
        <property type="entry name" value="ParB/Sulfiredoxin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036086">
        <property type="entry name" value="ParB/Sulfiredoxin_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02195">
        <property type="entry name" value="ParBc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00470">
        <property type="entry name" value="ParB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110849">
        <property type="entry name" value="SSF110849"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ParB" evidence="3">
        <location>
            <begin position="29"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="422"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="653"/>
            <end position="688"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="321"/>
            <end position="374"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00470"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF09906.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="688" mass="74753" checksum="82B4151DC6748AE0" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LIY4</accession>
    <name>Q1LIY4_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Thyroglobulin type-1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_3019</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09892.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3019"/>
    <dbReference type="EnsemblBacteria" id="ABF09892">
        <property type="protein sequence ID" value="ABF09892"/>
        <property type="gene ID" value="Rmet_3019"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3019"/>
    <dbReference type="HOGENOM" id="CLU_1947045_0_0_4"/>
    <dbReference type="OMA" id="HAPIQAC"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000716">
        <property type="entry name" value="Thyroglobulin_1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51162">
        <property type="entry name" value="THYROGLOBULIN_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thyroglobulin type-1" evidence="2">
        <location>
            <begin position="4"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="62"/>
            <end position="82"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF09892.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="129" mass="13772" checksum="6810F9FCB202476B" modified="2006-05-30" version="1">MSSRVQCERIRAALRAASGAAVPRAGVLPRTRLACGHCRVRRCAGCWCSLATGPQLPGSRTWGTGGGKCGRQDKRGRHLSARKASLHVGGRGTQRLCRRAAWGVRHAPIQACPRASHARTRQSLQGAQA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-10-17" modified="2022-05-25" version="95" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LJ33</accession>
    <name>PROA_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Gamma-glutamyl phosphate reductase</fullName>
            <shortName evidence="1">GPR</shortName>
            <ecNumber evidence="1">1.2.1.41</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Glutamate-5-semialdehyde dehydrogenase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Glutamyl-gamma-semialdehyde dehydrogenase</fullName>
            <shortName evidence="1">GSA dehydrogenase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">proA</name>
        <name type="ordered locus">Rmet_2970</name>
    </gene>
    <organism>
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the NADPH-dependent reduction of L-glutamate 5-phosphate into L-glutamate 5-semialdehyde and phosphate. The product spontaneously undergoes cyclization to form 1-pyrroline-5-carboxylate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>L-glutamate 5-semialdehyde + NADP(+) + phosphate = H(+) + L-glutamyl 5-phosphate + NADPH</text>
            <dbReference type="Rhea" id="RHEA:19541"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58066"/>
            <dbReference type="ChEBI" id="CHEBI:58274"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.2.1.41"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-proline biosynthesis; L-glutamate 5-semialdehyde from L-glutamate: step 2/2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the gamma-glutamyl phosphate reductase family.</text>
    </comment>
    <dbReference type="EC" id="1.2.1.41" evidence="1"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09843.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517506.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q1LJ33"/>
    <dbReference type="SMR" id="Q1LJ33"/>
    <dbReference type="STRING" id="266264.Rmet_2970"/>
    <dbReference type="EnsemblBacteria" id="ABF09843">
        <property type="protein sequence ID" value="ABF09843"/>
        <property type="gene ID" value="Rmet_2970"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2970"/>
    <dbReference type="eggNOG" id="COG0014">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030231_0_0_4"/>
    <dbReference type="OMA" id="PGVCNAM"/>
    <dbReference type="OrthoDB" id="223669at2"/>
    <dbReference type="UniPathway" id="UPA00098">
        <property type="reaction ID" value="UER00360"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004350">
        <property type="term" value="F:glutamate-5-semialdehyde dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055129">
        <property type="term" value="P:L-proline biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd07079">
        <property type="entry name" value="ALDH_F18-19_ProA-GPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.309.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.605.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00412">
        <property type="entry name" value="ProA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016161">
        <property type="entry name" value="Ald_DH/histidinol_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016163">
        <property type="entry name" value="Ald_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016162">
        <property type="entry name" value="Ald_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015590">
        <property type="entry name" value="Aldehyde_DH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020593">
        <property type="entry name" value="G-glutamylP_reductase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012134">
        <property type="entry name" value="Glu-5-SA_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000965">
        <property type="entry name" value="GPR_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00171">
        <property type="entry name" value="Aldedh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000151">
        <property type="entry name" value="GPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53720">
        <property type="entry name" value="SSF53720"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00407">
        <property type="entry name" value="proA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01223">
        <property type="entry name" value="PROA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0521">NADP</keyword>
    <keyword id="KW-0560">Oxidoreductase</keyword>
    <keyword id="KW-0641">Proline biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000252583" description="Gamma-glutamyl phosphate reductase">
        <location>
            <begin position="1"/>
            <end position="426"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00412"/>
        </source>
    </evidence>
    <sequence length="426" mass="45730" checksum="93B9E798FE2362ED" modified="2006-05-30" version="1">MTEFDLHAYMTRVGQQARAASRAMARASTADKNRALLTIAAAIRRDADKLKAVNARDVERARTNGQDAAFIDRLTLSDKAIATMAAGLEQIAALADPIGEISNMKFRPTGIQVGQMRVPLGVIGIIYESRPNVTIDAAALCLKSGNSTILRGGSEAIESNTALAALVAEGLAAAGLPSEAVQVIETTDRAAVGRLITMTEYVDVIVPRGGKSLIARLMEEARVPMIKHLDGICHVYIDDDADLEKAVRVCDNAKTQRYAPCNTMETLLVSREIAAKALPPLCRIYQEKGVELRVCPATRATLEAAGFSGLVDATEEDWRLEYLAPVLAIRTVDGLDAAIAHINTYGSAHTDSIITENYTTGMRFLREVDSASVMINASTRFADGFEYGLGAEIGISNDKLHARGPVGLEGLTSLKYVVFGHGEIRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="113" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LJC9</accession>
    <name>Q1LJC9_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 10">Quinolinate synthase</fullName>
            <ecNumber evidence="2 10">2.5.1.72</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10 11" type="primary">nadA</name>
        <name evidence="11" type="ordered locus">Rmet_2874</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Catalyzes the condensation of iminoaspartate with dihydroxyacetone phosphate to form quinolinate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>dihydroxyacetone phosphate + iminosuccinate = H(+) + 2 H2O + phosphate + quinolinate</text>
            <dbReference type="Rhea" id="RHEA:25888"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29959"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57642"/>
            <dbReference type="ChEBI" id="CHEBI:77875"/>
            <dbReference type="EC" id="2.5.1.72"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="10">Binds 1 [4Fe-4S] cluster per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1 10">Cofactor biosynthesis; NAD(+) biosynthesis; quinolinate from iminoaspartate: step 1/1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the quinolinate synthase family. Type 1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.72" evidence="2 10"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09747.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517426.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2874"/>
    <dbReference type="EnsemblBacteria" id="ABF09747">
        <property type="protein sequence ID" value="ABF09747"/>
        <property type="gene ID" value="Rmet_2874"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2874"/>
    <dbReference type="eggNOG" id="COG0379">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047382_1_0_4"/>
    <dbReference type="OMA" id="CFCSTMN"/>
    <dbReference type="OrthoDB" id="613347at2"/>
    <dbReference type="UniPathway" id="UPA00253">
        <property type="reaction ID" value="UER00327"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008987">
        <property type="term" value="F:quinolinate synthetase A activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009435">
        <property type="term" value="P:NAD biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10800">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00567">
        <property type="entry name" value="NadA_type1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003473">
        <property type="entry name" value="NadA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036094">
        <property type="entry name" value="NadA_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023513">
        <property type="entry name" value="Quinolinate_synth_A_type1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30573">
        <property type="entry name" value="PTHR30573"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02445">
        <property type="entry name" value="NadA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF142754">
        <property type="entry name" value="SSF142754"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00550">
        <property type="entry name" value="nadA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 10" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="4 10" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8 10" id="KW-0408">Iron</keyword>
    <keyword evidence="9 10" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="7 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="5 10" id="KW-0662">Pyridine nucleotide biosynthesis</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 10 11" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Iminoaspartate binding" evidence="10">
        <location>
            <begin position="157"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="region of interest" description="Iminoaspartate binding" evidence="10">
        <location>
            <begin position="244"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="65"/>
        </location>
        <ligand>
            <name>iminosuccinate</name>
            <dbReference type="ChEBI" id="CHEBI:77875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="86"/>
        </location>
        <ligand>
            <name>iminosuccinate</name>
            <dbReference type="ChEBI" id="CHEBI:77875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="131"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="174"/>
        </location>
        <ligand>
            <name>iminosuccinate</name>
            <dbReference type="ChEBI" id="CHEBI:77875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="218"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="261"/>
        </location>
        <ligand>
            <name>iminosuccinate</name>
            <dbReference type="ChEBI" id="CHEBI:77875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="315"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012669"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00567"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ABF09747.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="384" mass="41438" checksum="9B09C4BD4C736272" modified="2006-05-30" version="1">MTPQTIKSVEFEKPNLTDPDAAAGGSCVAHAWAKVPPALTPDERQALKSRIRKLLKERNAVLVAHYYVDADLQDLAEETGGCVSDSLEMARFGRDHDAKTLVVAGVRFMGETAKILSPEKTILMPDLDATCSLDLGCPADDFSAFCDAHPDRTVVVYANTSAAVKARADWMVTSSIGLKIVEHLHARGEKILWAPDKHLGSYIQQQTGADMLLWQGSCLVHDEFKGIELDLLRREHPKAKILVHPESPANVVAQADVVGSTSQLIAAAQQLDANEFIVATDNGILHKMRMAAPGKMFIEAPTAGNSATCKSCAHCPWMAMNALTNLAEVLETGRNEIHVDADIGRRAVTCINRMLDFAAQQKANVRPAADLAKESALFSGVGPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LJF3</accession>
    <name>Q1LJF3_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">N-Formylglutamate amidohydrolase</fullName>
            <ecNumber evidence="1">3.5.1.68</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">hutG1</name>
        <name evidence="1" type="ordered locus">Rmet_2850</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EC" id="3.5.1.68" evidence="1"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09723.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008644124.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2850"/>
    <dbReference type="EnsemblBacteria" id="ABF09723">
        <property type="protein sequence ID" value="ABF09723"/>
        <property type="gene ID" value="Rmet_2850"/>
    </dbReference>
    <dbReference type="GeneID" id="60820711"/>
    <dbReference type="KEGG" id="rme:Rmet_2850"/>
    <dbReference type="eggNOG" id="COG3741">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069318_0_0_4"/>
    <dbReference type="OMA" id="WHIPQLY"/>
    <dbReference type="OrthoDB" id="813848at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050129">
        <property type="term" value="F:N-formylglutamate deformylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010247">
        <property type="entry name" value="HutG_amidohyd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007709">
        <property type="entry name" value="N-FG_amidohydro"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05013">
        <property type="entry name" value="FGase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02017">
        <property type="entry name" value="hutG_amidohyd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF09723.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="271" mass="29955" checksum="E13FA72BBF9AA33F" modified="2006-05-30" version="1">MSFTTDIPAFTLHRGTRPLLVSMPHVGTHLPAKLAERLTPEARTVPDTDWHMDRVYECARELGASILMATHSRYVIDLNRPPDNQNLYPGQDTTGLCPVDTFDKTPIYADGTNGPDDAEVALRRGAVWHPYHNALQEELARLRAEHGTIGLWEAHSIRSMLPRFFEGKLPDFNLGTADGKSCDAGLAAELIAIADAVPNHTAVLNGRFKGGYITRQYGNPAQGVHAVQLEQTQSSYMEEVYPFAYSEAKAAAIQPAIRKMLETMLAFVEAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LJW1</accession>
    <name>Q1LJW1_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Alpha-methylacyl-CoA racemase</fullName>
            <ecNumber evidence="1">5.1.99.4</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_2692</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EC" id="5.1.99.4" evidence="1"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09565.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517265.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2692"/>
    <dbReference type="EnsemblBacteria" id="ABF09565">
        <property type="protein sequence ID" value="ABF09565"/>
        <property type="gene ID" value="Rmet_2692"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2692"/>
    <dbReference type="eggNOG" id="COG1804">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033975_5_0_4"/>
    <dbReference type="OMA" id="FHDSPFY"/>
    <dbReference type="OrthoDB" id="969868at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008111">
        <property type="term" value="F:alpha-methylacyl-CoA racemase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1540.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10540">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003673">
        <property type="entry name" value="CoA-Trfase_fam_III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044855">
        <property type="entry name" value="CoA-Trfase_III_dom3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023606">
        <property type="entry name" value="CoA-Trfase_III_dom_1_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02515">
        <property type="entry name" value="CoA_transf_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89796">
        <property type="entry name" value="SSF89796"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0413">Isomerase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF09565.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="344" mass="35968" checksum="E7D29B4CA4E9E65E" modified="2006-05-30" version="1">MTPPLQGIRIVEFEGIGPGPLAGRMLADMGAEVTVIARRQRVAVADQLGGDKPSPLRRGKVIVPLDLKQPDDLAEATRLVDGADALIEGNRPGVMERLGLGPADCAARNPKLVYGRMTGWGQSGPLAQTAGHDLNYVALTGMLSLSARPGEAPMIPPTVVGDAGGALGLAFGVVCALLDARASGRGRVVDGAIVDMVAMLGSIAVWVRNNGQLDGPTPSPFHQSPFYDVYGCADGGFITIGALEPQFYALLLERLGMTDVDPAAQYDHAAWPALKQRFTRLFASEPRAHWDALLGGTDVCYAPVLSIAEASRHPHNVARGVFIAQEDGALDVAGAPRFAPSGAA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-10-31" modified="2022-08-03" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LK07</accession>
    <name>PDXH_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Pyridoxine/pyridoxamine 5'-phosphate oxidase</fullName>
            <ecNumber evidence="1">1.4.3.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">PNP/PMP oxidase</fullName>
            <shortName evidence="1">PNPOx</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Pyridoxal 5'-phosphate synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">pdxH</name>
        <name type="ordered locus">Rmet_2642</name>
    </gene>
    <organism>
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the oxidation of either pyridoxine 5'-phosphate (PNP) or pyridoxamine 5'-phosphate (PMP) into pyridoxal 5'-phosphate (PLP).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>H2O + O2 + pyridoxamine 5'-phosphate = H2O2 + NH4(+) + pyridoxal 5'-phosphate</text>
            <dbReference type="Rhea" id="RHEA:15817"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:58451"/>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
            <dbReference type="EC" id="1.4.3.5"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>O2 + pyridoxine 5'-phosphate = H2O2 + pyridoxal 5'-phosphate</text>
            <dbReference type="Rhea" id="RHEA:15149"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:58589"/>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
            <dbReference type="EC" id="1.4.3.5"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
        <text evidence="1">Binds 1 FMN per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor metabolism; pyridoxal 5'-phosphate salvage; pyridoxal 5'-phosphate from pyridoxamine 5'-phosphate: step 1/1.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor metabolism; pyridoxal 5'-phosphate salvage; pyridoxal 5'-phosphate from pyridoxine 5'-phosphate: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the pyridoxamine 5'-phosphate oxidase family.</text>
    </comment>
    <dbReference type="EC" id="1.4.3.5" evidence="1"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09519.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517218.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q1LK07"/>
    <dbReference type="SMR" id="Q1LK07"/>
    <dbReference type="STRING" id="266264.Rmet_2642"/>
    <dbReference type="EnsemblBacteria" id="ABF09519">
        <property type="protein sequence ID" value="ABF09519"/>
        <property type="gene ID" value="Rmet_2642"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2642"/>
    <dbReference type="eggNOG" id="COG0259">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032263_2_2_4"/>
    <dbReference type="OMA" id="PEPNAMV"/>
    <dbReference type="OrthoDB" id="1542844at2"/>
    <dbReference type="UniPathway" id="UPA01068">
        <property type="reaction ID" value="UER00304"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA01068">
        <property type="reaction ID" value="UER00305"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004733">
        <property type="term" value="F:pyridoxamine-phosphate oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008615">
        <property type="term" value="P:pyridoxine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.110.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01629">
        <property type="entry name" value="PdxH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000659">
        <property type="entry name" value="Pyridox_Oxase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019740">
        <property type="entry name" value="Pyridox_Oxase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011576">
        <property type="entry name" value="Pyridox_Oxase_put"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019576">
        <property type="entry name" value="Pyridoxamine_oxidase_dimer_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012349">
        <property type="entry name" value="Split_barrel_FMN-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10851">
        <property type="entry name" value="PTHR10851"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10590">
        <property type="entry name" value="PNP_phzG_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01243">
        <property type="entry name" value="Putative_PNPOx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000190">
        <property type="entry name" value="Pyd_amn-ph_oxd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00558">
        <property type="entry name" value="pdxH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01064">
        <property type="entry name" value="PYRIDOX_OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0285">Flavoprotein</keyword>
    <keyword id="KW-0288">FMN</keyword>
    <keyword id="KW-0560">Oxidoreductase</keyword>
    <keyword id="KW-0664">Pyridoxine biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000255878" description="Pyridoxine/pyridoxamine 5'-phosphate oxidase">
        <location>
            <begin position="1"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="8"/>
            <end position="11"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="61"/>
            <end position="66"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="66"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="76"/>
            <end position="77"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="82"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="83"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="105"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="123"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="127"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="131"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="140"/>
            <end position="141"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="184"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="190"/>
            <end position="192"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="194"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01629"/>
        </source>
    </evidence>
    <sequence length="212" mass="24190" checksum="52389D9BD48D2084" modified="2006-05-30" version="1">MTQLADLRRNYMLSALSETDVAPDPIRQFQSWFDEAMQAKLPEPNAMTLATVGADGQPSARIVLLKGMDADGFTFFTNYESRKGVDLLANPRAALLFHWVQLERQIRVEGIVEKVEDAESDAYYASRPLGSRLGAWASEQSREVAGRDVIEAREADFRNKFGENPPRPPHWGGYRLKPTWIEFWQGRPSRLHDRIAYRQNADGNWQIVRLSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="95" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LK75</accession>
    <name>Q1LK75_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Oligoribonuclease</fullName>
            <ecNumber evidence="1">3.1.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1 3" type="primary">orn</name>
        <name evidence="3" type="ordered locus">Rmet_2574</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">3'-to-5' exoribonuclease specific for small oligoribonucleotides.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the oligoribonuclease family.</text>
    </comment>
    <dbReference type="EC" id="3.1.-.-" evidence="1"/>
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        <property type="protein sequence ID" value="ABF09451.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517154.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2574"/>
    <dbReference type="EnsemblBacteria" id="ABF09451">
        <property type="protein sequence ID" value="ABF09451"/>
        <property type="gene ID" value="Rmet_2574"/>
    </dbReference>
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    <dbReference type="eggNOG" id="COG1949">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_064761_2_0_4"/>
    <dbReference type="OMA" id="AFFHYRN"/>
    <dbReference type="OrthoDB" id="1390327at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000175">
        <property type="term" value="F:3'-5'-exoribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06135">
        <property type="entry name" value="Orn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00045">
        <property type="entry name" value="Oligoribonuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013520">
        <property type="entry name" value="Exonuclease_RNaseT/DNA_pol3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022894">
        <property type="entry name" value="Oligoribonuclease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11046">
        <property type="entry name" value="PTHR11046"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00929">
        <property type="entry name" value="RNase_T"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00479">
        <property type="entry name" value="EXOIII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="SSF53098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="1" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="1 3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0540">Nuclease</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Exonuclease" evidence="2">
        <location>
            <begin position="14"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="136"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00045"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF09451.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="205" mass="22979" checksum="0CB3A2C780E4BC9C" modified="2006-05-30" version="1">MTSPSASSVKSENNLVWLDMEMTGLQPDTDRIIEVAVVVTDSELNILAEGPVLVIHQPDAVLDAMDNWNKGTHGRSGLIEKVKASTLTEAQAEAELLAFLKRWVPANKSPMCGNSICQDRRFMARYMPKLESFFHYRNLDVSTLKELCKRWEPAIHKGFTKRQLHTALADILESVEELRYYRQHFIKTSASATPEAPEAPQAPAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="111" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LKB5</accession>
    <name>Q1LKB5_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative ATP-dependent transcriptional regulator, LuxR-family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_2534</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09411.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517110.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2534"/>
    <dbReference type="EnsemblBacteria" id="ABF09411">
        <property type="protein sequence ID" value="ABF09411"/>
        <property type="gene ID" value="Rmet_2534"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2534"/>
    <dbReference type="eggNOG" id="COG2909">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006325_3_1_4"/>
    <dbReference type="OMA" id="VEHWVIT"/>
    <dbReference type="OrthoDB" id="1377603at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06170">
        <property type="entry name" value="LuxR_C_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016032">
        <property type="entry name" value="Sig_transdc_resp-reg_C-effctor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041617">
        <property type="entry name" value="TPR_MalT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000792">
        <property type="entry name" value="Tscrpt_reg_LuxR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00196">
        <property type="entry name" value="GerE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17874">
        <property type="entry name" value="TPR_MalT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00038">
        <property type="entry name" value="HTHLUXR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00421">
        <property type="entry name" value="HTH_LUXR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46894">
        <property type="entry name" value="SSF46894"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50043">
        <property type="entry name" value="HTH_LUXR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH luxR-type" evidence="2">
        <location>
            <begin position="848"/>
            <end position="913"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF09411.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="925" mass="101439" checksum="1E22AECBE5C90929" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="96" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LLB4</accession>
    <name>Q1LLB4_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 7">Phosphate transport system permease protein PstA</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">pstA</name>
        <name evidence="9" type="ordered locus">Rmet_2183</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cell inner membrane</location>
            <topology evidence="7">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the binding-protein-dependent transport system permease family. CysTW subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09062.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516890.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2183"/>
    <dbReference type="EnsemblBacteria" id="ABF09062">
        <property type="protein sequence ID" value="ABF09062"/>
        <property type="gene ID" value="Rmet_2183"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2183"/>
    <dbReference type="eggNOG" id="COG0581">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033621_2_0_4"/>
    <dbReference type="OMA" id="GIARWKA"/>
    <dbReference type="OrthoDB" id="1763210at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005887">
        <property type="term" value="C:integral component of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005315">
        <property type="term" value="F:inorganic phosphate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035435">
        <property type="term" value="P:phosphate ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005672">
        <property type="entry name" value="Phosphate_PstA"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="SSF161098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00974">
        <property type="entry name" value="3a0107s02c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="36"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="90"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="128"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="159"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="214"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="275"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="8">
        <location>
            <begin position="91"/>
            <end position="294"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00016864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU363043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ABF09062.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="300" mass="32053" checksum="6BCD4CF9B78563C3" modified="2006-05-30" version="1">MNQASQAVSTSSIPAVRPDSDAVRTRLQGSRRRMNMYALTASLAAMGFGLFWLAWILWTTISLGVGGLSLDLFTQMTPAPNTAGGGLANAIFGSFVLVGMATLVGTPLGILAGIYLAEYGKKSPLASLIRFINDILLSAPSIVIGLFVYAIVVTRMGHFSGWAGVCALALLQVPIVVRTTENMLNLVPNALREAAVALGTPKWKMVMSITIKSSYAGIVTGVLLAVARIAGETAPLLFTALSNQFWSADLNKPMANLPVTIFRFAMSPFTEWQQLAWAGVFLITIGVLALNIIARMLFKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="109" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LLL1</accession>
    <name>Q1LLL1_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Sensor protein</fullName>
            <ecNumber evidence="14">2.7.13.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="primary">narX</name>
        <name evidence="19" type="ordered locus">Rmet_2086</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.</text>
            <dbReference type="EC" id="2.7.13.3"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell inner membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.7.13.3" evidence="14"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08965.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516800.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2086"/>
    <dbReference type="EnsemblBacteria" id="ABF08965">
        <property type="protein sequence ID" value="ABF08965"/>
        <property type="gene ID" value="Rmet_2086"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2086"/>
    <dbReference type="eggNOG" id="COG3850">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_20_10_4"/>
    <dbReference type="OMA" id="IKHSQGT"/>
    <dbReference type="OrthoDB" id="1755994at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.960">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003018">
        <property type="entry name" value="GAF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029016">
        <property type="entry name" value="GAF-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003660">
        <property type="entry name" value="HAMP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029095">
        <property type="entry name" value="NarX-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042295">
        <property type="entry name" value="NarX-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016380">
        <property type="entry name" value="Sig_transdc_His_kin_NarX/NarQ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011712">
        <property type="entry name" value="Sig_transdc_His_kin_sub3_dim/P"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13185">
        <property type="entry name" value="GAF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00672">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07730">
        <property type="entry name" value="HisKA_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13675">
        <property type="entry name" value="PilJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF003167">
        <property type="entry name" value="STHK_NarX/NarQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00065">
        <property type="entry name" value="GAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00304">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="SSF55874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50885">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 14" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="4 14" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="15" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="10 14 19" id="KW-0418">Kinase</keyword>
    <keyword evidence="13 14 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="9 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 14" id="KW-0808">Transferase</keyword>
    <keyword evidence="8 16 19" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 16" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="12 14" id="KW-0902">Two-component regulatory system</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="36"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="192"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="domain" description="HAMP" evidence="18">
        <location>
            <begin position="211"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="17">
        <location>
            <begin position="462"/>
            <end position="655"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="15">
        <location>
            <begin position="255"/>
            <end position="282"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004429"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRNR" id="PIRNR003167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="ABF08965.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="671" mass="73563" checksum="1E032B14D14B5940" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="72" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LLX4</accession>
    <name>Q1LLX4_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Phosphate transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">pitA</name>
        <name evidence="7" type="ordered locus">Rmet_1973</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08852.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008650677.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1973"/>
    <dbReference type="EnsemblBacteria" id="ABF08852">
        <property type="protein sequence ID" value="ABF08852"/>
        <property type="gene ID" value="Rmet_1973"/>
    </dbReference>
    <dbReference type="GeneID" id="60821421"/>
    <dbReference type="KEGG" id="rme:Rmet_1973"/>
    <dbReference type="eggNOG" id="COG0306">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015355_1_1_4"/>
    <dbReference type="OMA" id="RANPHKA"/>
    <dbReference type="OrthoDB" id="1329125at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005315">
        <property type="term" value="F:inorganic phosphate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006817">
        <property type="term" value="P:phosphate ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001204">
        <property type="entry name" value="Phos_transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11101">
        <property type="entry name" value="PTHR11101"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01384">
        <property type="entry name" value="PHO4"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="49"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="84"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="111"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="139"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="222"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="265"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="311"/>
            <end position="331"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABF08852.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="336" mass="35203" checksum="89C090E69F5B6D8E" modified="2006-05-30" version="1">MHTVQMSLWVIGLLVALALLFDFMNGFHDAANSIATVVSTGVLKPHHAVAMAAMCNVIAIFIFHLKVAATVGTGTIDVNIVDHYVIFGALVGAIAWNVITWLYGIPSSSSHALIGGLVGAAVAKAGTGALVGNGLLKTVAFILISPLLGFILGSIMMVIVGWTFFRTPPSRVDRWFRRLQLVSASLYSLGHGGNDAQKTIGIIWMLLIASGHVAVGGAAPPMWVIVSCYLAIGMGTLFGGWRIVRTMGQKITKLKPVGGFCAETGGAITLFIASALGVPVSTTHTITGAIVGVGSAQKMSAVRWGVAGNIVWAWVLTIPASAFMSAIAWWIGKQIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LM25</accession>
    <name>Q1LM25_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Thioredoxin reductase</fullName>
            <ecNumber evidence="2">1.8.1.9</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_1922</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EC" id="1.8.1.9" evidence="2"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08801.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516654.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="SMR" id="Q1LM25"/>
    <dbReference type="STRING" id="266264.Rmet_1922"/>
    <dbReference type="EnsemblBacteria" id="ABF08801">
        <property type="protein sequence ID" value="ABF08801"/>
        <property type="gene ID" value="Rmet_1922"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1922"/>
    <dbReference type="eggNOG" id="COG0492">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031864_5_8_4"/>
    <dbReference type="OrthoDB" id="692968at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004791">
        <property type="term" value="F:thioredoxin-disulfide reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018490">
        <property type="entry name" value="cNMP-bd-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023753">
        <property type="entry name" value="FAD/NAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07992">
        <property type="entry name" value="Pyr_redox_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51206">
        <property type="entry name" value="SSF51206"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="SSF51905"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="1">
        <location>
            <begin position="36"/>
            <end position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF08801.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="550" mass="57652" checksum="F44DBB5D7AF75509" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LMK8</accession>
    <name>Q1LMK8_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_1739</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08618.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516470.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1739"/>
    <dbReference type="EnsemblBacteria" id="ABF08618">
        <property type="protein sequence ID" value="ABF08618"/>
        <property type="gene ID" value="Rmet_1739"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1739"/>
    <dbReference type="eggNOG" id="COG1331">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_043770_0_0_4"/>
    <dbReference type="OMA" id="WVEGTAQ"/>
    <dbReference type="OrthoDB" id="1048867at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008928">
        <property type="entry name" value="6-hairpin_glycosidase_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48208">
        <property type="entry name" value="SSF48208"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193566" evidence="1">
        <location>
            <begin position="34"/>
            <end position="451"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF08618.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="451" mass="48573" checksum="A1149BA545C74D7B" modified="2006-05-30" version="1">MQRHTSKGLAAGGIRLATYLATSCILATSPTFAASSADTRTQLRDSGCQTLAQRVTAIPGSGPIWLASYEPAPGGEPLPAPLRQSAFVYDNALAGIALIACGQRDAARRIADGVLAGMSRDRHYHDGRLRNAYRAGAIPNGPVPLPGWWDDASKRWFEDRYQVGTATGNVVWAALLLLATYEATRAPAYLDGAKALMGWVDKETFNADSPAAYIGGYFGHEPNPERQGWKSTEHNVDAYAAFRWLARYSNDPRWNTDAERARHFVESMWQSREGRFAIGTRDDGRTLNGGPSALDASLWPLIAIPDAAGTWQRSLDWARTRHAVNGGFGFKADPDGVWTEGTGQAALVLLATNHADVAGPLWPLLLSQRAPDGMLFATPEKRIQTGLSIGPDSKTEDFYYFHLPHLGATAWAVLAATGWNPFQPRGCRANCAATTASVSGPSNGPANGPNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="96" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LMM3</accession>
    <name>Q1LMM3_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Acyl-CoA dehydrogenase, long-chain specific</fullName>
            <ecNumber evidence="8">1.3.99.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Rmet_1722</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the acyl-CoA dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.3.99.-" evidence="8"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08603.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516456.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1722"/>
    <dbReference type="EnsemblBacteria" id="ABF08603">
        <property type="protein sequence ID" value="ABF08603"/>
        <property type="gene ID" value="Rmet_1722"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1722"/>
    <dbReference type="eggNOG" id="COG1960">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018204_0_3_4"/>
    <dbReference type="OMA" id="NECAQCA"/>
    <dbReference type="OrthoDB" id="760677at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003995">
        <property type="term" value="F:acyl-CoA dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.540.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.110.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006089">
        <property type="entry name" value="Acyl-CoA_DH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006091">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046373">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036250">
        <property type="entry name" value="AcylCo_DH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009075">
        <property type="entry name" value="AcylCo_DH/oxidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013786">
        <property type="entry name" value="AcylCoA_DH/ox_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037069">
        <property type="entry name" value="AcylCoA_DH/ox_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009100">
        <property type="entry name" value="AcylCoA_DH/oxidase_NM_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00441">
        <property type="entry name" value="Acyl-CoA_dh_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02770">
        <property type="entry name" value="Acyl-CoA_dh_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02771">
        <property type="entry name" value="Acyl-CoA_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47203">
        <property type="entry name" value="SSF47203"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56645">
        <property type="entry name" value="SSF56645"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00072">
        <property type="entry name" value="ACYL_COA_DH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00073">
        <property type="entry name" value="ACYL_COA_DH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0274">FAD</keyword>
    <keyword evidence="3" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Acyl-CoA_dh_N" evidence="7">
        <location>
            <begin position="4"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA_dh_M" evidence="6">
        <location>
            <begin position="122"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA_dh_1" evidence="5">
        <location>
            <begin position="233"/>
            <end position="380"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF02770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF02771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ABF08603.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="388" mass="42853" checksum="6B38E8A9BBFAA46F" modified="2006-05-30" version="1">MLYTQDHRSIMDSIRRFVAAEIDPFVDEWEAAETFPAHALFRKMGELGFLGITKPVEYGGLGLDFSYAVAAAEALGHARAQGVGMGIGVQTDMATPALAAFGTDALKREFLAPAIAGEMVVSIGVSEVGAGSDVASLKTRARRDGDDYVISGSKMWITNGTQADWICLLCNTSDGPVHKNKSLIIVPLRENGRLVRGVEVQKIRKMGMWCSDTAQIFFDDVRVPARNRIGDEGMGFIYQMKQFQEERLNGAARRLACSALIEETADYLRQRVAFGKPLLDNQYIQFKLAELKTELESLRALVHVATRAYIQGEDVVELASMAKLKAGRLSREIADWCIQFQGGMGYTWDNHANRAYRDFRLGSIGGGADEVMLQVIAKQMGLMSRHDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-10-07" version="53" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LMR7</accession>
    <name>Q1LMR7_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_1678</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08559.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1678"/>
    <dbReference type="EnsemblBacteria" id="ABF08559">
        <property type="protein sequence ID" value="ABF08559"/>
        <property type="gene ID" value="Rmet_1678"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1678"/>
    <dbReference type="HOGENOM" id="CLU_1667953_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF08559.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="158" mass="17566" checksum="E58CAF2AECBD66F6" modified="2006-05-30" version="1">MAKSSRPRKPYRPRQTEAHLTGRLQPWVLDKLFAPLESMLQHIRDGGELEVDDDEQLWYYSTTMGGSFKCAELLESFGDIFARQRQRDTHAPDPEALYAMAASMTAEILAMREVEAAIACASEMRGYMAASSLGNMKDAVLLASLDSHMTTVYGQGDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LN61</accession>
    <name>Q1LN61_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7 15">NAD(P) transhydrogenase subunit beta</fullName>
            <ecNumber evidence="6 15">7.1.1.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">Nicotinamide nucleotide transhydrogenase subunit beta</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="primary">pntB</name>
        <name evidence="18" type="ordered locus">Rmet_1532</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2 15">The transhydrogenation between NADH and NADP is coupled to respiration and ATP hydrolysis and functions as a proton pump across the membrane.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 15">
            <text>H(+)(in) + NAD(+) + NADPH = H(+)(out) + NADH + NADP(+)</text>
            <dbReference type="Rhea" id="RHEA:47992"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="7.1.1.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cell inner membrane</location>
            <topology evidence="4">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5 15">Belongs to the PNT beta subunit family.</text>
    </comment>
    <dbReference type="EC" id="7.1.1.1" evidence="6 15"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08415.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516272.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1532"/>
    <dbReference type="EnsemblBacteria" id="ABF08415">
        <property type="protein sequence ID" value="ABF08415"/>
        <property type="gene ID" value="Rmet_1532"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1532"/>
    <dbReference type="eggNOG" id="COG1282">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007866_4_0_4"/>
    <dbReference type="OMA" id="NCRMLYG"/>
    <dbReference type="OrthoDB" id="788546at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008750">
        <property type="term" value="F:NAD(P)+ transhydrogenase (AB-specific) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029035">
        <property type="entry name" value="DHS-like_NAD/FAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012136">
        <property type="entry name" value="NADH_DH_b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034300">
        <property type="entry name" value="PNTB-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02233">
        <property type="entry name" value="PNTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000204">
        <property type="entry name" value="PNTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52467">
        <property type="entry name" value="SSF52467"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 15" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="8 15" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="14 15 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-0520">NAD</keyword>
    <keyword evidence="11 15" id="KW-0521">NADP</keyword>
    <keyword evidence="18" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12 15" id="KW-1278">Translocase</keyword>
    <keyword evidence="10 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="13 16" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="6"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="32"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="55"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="86"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="112"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="155"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="181"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="232"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="domain" description="PNTB" evidence="17">
        <location>
            <begin position="6"/>
            <end position="450"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00003943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004429"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00007919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00014581"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRNR" id="PIRNR000204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF02233"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="ABF08415.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="457" mass="47016" checksum="C999D0DAECE0CC63" modified="2006-05-30" version="1">MSDVIQLAYFAVAVVFILGLKAMSSPVSARKGIVWAGYAMVAATLITLLKPGMENYGLMITAIVLGGAVAWWSGKVVKMTDMPQMVAIYNGMGSGAAAAIAALEFARGSMHGGFAATLAVLGALIGSVAFSGSCIAFAKLQGIMTKAWRLPAQNVVNVALAGIALVLGGAIIFGSTPASEMVIAFFVVALALGAILTSPIGGADMPVVISLLNAFTGLAVGFEGYVLGNPALIVAGIVVGASGTLLTQLMAKAMNRPIKNIIFTPITGAAAGGGEAIGGSMKELSALDGASVMRYASKVIIVPGYGMAVAGAQHKVWEMAQLLEEGGVEVVFAIHPVAGRMPGHMNVLLAEAGVPYDKIFDLEEINADFPQADVALVIGANDVVNPVARTDKSSPIYGMPILNVDMAQNVIVVKRGQGAGYSGIENALFFKDNCRMLYGSAQQIIGEVITHVKALEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="65" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LND4</accession>
    <name>Q1LND4_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DUF4124 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Rmet_1459</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08342.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516208.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1459"/>
    <dbReference type="EnsemblBacteria" id="ABF08342">
        <property type="protein sequence ID" value="ABF08342"/>
        <property type="gene ID" value="Rmet_1459"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1459"/>
    <dbReference type="HOGENOM" id="CLU_108835_0_1_4"/>
    <dbReference type="OMA" id="ASAQWQW"/>
    <dbReference type="OrthoDB" id="2045232at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025392">
        <property type="entry name" value="DUF4124"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13511">
        <property type="entry name" value="DUF4124"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193359" description="DUF4124 domain-containing protein" evidence="3">
        <location>
            <begin position="24"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="domain" description="DUF4124" evidence="4">
        <location>
            <begin position="12"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="65"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="107"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF13511"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABF08342.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="179" mass="19253" checksum="21BEC11B118BF240" modified="2006-05-30" version="1">MNRSSVLYLLIAASLAVPGTAFSQWQWKDTNGRTVYSDTPPPPSVPDRAVIMAPGRAPGMYHAVEADAPPKGDAPAKADAPRMQPTSVKAGGAKPKDADTEFQERRDARLKADLEAATKEREQLARQQSCQELRNYAAGLNQGMRAAKAGPDGVPVRLNDDERAAEIARANEALQQCNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="95" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LNH6</accession>
    <name>Q1LNH6_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Cytochrome c, class I</fullName>
            <ecNumber evidence="13">1.1.99.3</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ordered locus">Rmet_1417</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>heme c</name>
            <dbReference type="ChEBI" id="CHEBI:61717"/>
        </cofactor>
        <text evidence="8">Binds 3 heme c groups covalently per subunit.</text>
    </comment>
    <comment type="PTM">
        <text evidence="9">Binds 3 heme groups per subunit.</text>
    </comment>
    <dbReference type="EC" id="1.1.99.3" evidence="13"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08300.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516171.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1417"/>
    <dbReference type="EnsemblBacteria" id="ABF08300">
        <property type="protein sequence ID" value="ABF08300"/>
        <property type="gene ID" value="Rmet_1417"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1417"/>
    <dbReference type="eggNOG" id="COG2010">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028594_0_1_4"/>
    <dbReference type="OMA" id="CHTPRGM"/>
    <dbReference type="OrthoDB" id="1297285at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033717">
        <property type="term" value="F:gluconate 2-dehydrogenase (acceptor) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.760.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009056">
        <property type="entry name" value="Cyt_c-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036909">
        <property type="entry name" value="Cyt_c-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014353">
        <property type="entry name" value="Membr-bd_ADH_cyt_c"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00034">
        <property type="entry name" value="Cytochrom_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13442">
        <property type="entry name" value="Cytochrome_CBB3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000018">
        <property type="entry name" value="Mb_ADH_cyt_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46626">
        <property type="entry name" value="SSF46626"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51007">
        <property type="entry name" value="CYTC"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="2 8 10" id="KW-0349">Heme</keyword>
    <keyword evidence="6 9 10" id="KW-0408">Iron</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 9 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 11" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193362" evidence="11">
        <location>
            <begin position="32"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="domain" description="Cytochrome c" evidence="12">
        <location>
            <begin position="44"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="domain" description="Cytochrome c" evidence="12">
        <location>
            <begin position="180"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="domain" description="Cytochrome c" evidence="12">
        <location>
            <begin position="310"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="binding site" description="covalent" evidence="8">
        <location>
            <position position="58"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" description="covalent" evidence="8">
        <location>
            <position position="61"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="9">
        <location>
            <position position="62"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
            <label>1</label>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <feature type="binding site" description="covalent" evidence="8">
        <location>
            <position position="203"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" description="covalent" evidence="8">
        <location>
            <position position="206"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="9">
        <location>
            <position position="207"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
            <label>2</label>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <feature type="binding site" description="covalent" evidence="8">
        <location>
            <position position="323"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
            <label>3</label>
        </ligand>
    </feature>
    <feature type="binding site" description="covalent" evidence="8">
        <location>
            <position position="326"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
            <label>3</label>
        </ligand>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="9">
        <location>
            <position position="327"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
            <label>3</label>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR000018-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR000018-51"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ABF08300.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="421" mass="44660" checksum="89E3687C113D6352" modified="2006-05-30" version="1">MTTIRWISALAAGSALLLAGCGSGSSDVPQAADLAPATKLTPAQQIERGRYLVRAADCAACHTAPNGAPFAGGVELASPFGKFYGTNITPDKDHGIGKWSAADFYKALHDGVAPDKHLYPAMPYTSYRGLSRTDTDAMYAYLMQLKPMAVQNREPELGFPYNQRFAMAGWNLLFRKDALPDASKGESPVWIRGRYLTNALGHCAECHTPRGKFGQMDGGRPLTGSALARVGAPDISPAGLAARGWTAADLQTFFSTGIAPQGSAYGEMFPVVHLSSRYLNAADVGAMSTYLLGDKPPVPQPLKPANADAGAMATGRQHYLAVCAGCHGREGEGKPHVAVAMLGNSTVRNADPRNLIVSMLDGIERQDFPGLESMQDMPGFATRMNDAELAALANYLRASYGGQPADVTADSVKVLRETPSH</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-05-26" modified="2022-08-03" version="102" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LNN2</accession>
    <name>RIMO_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Ribosomal protein S12 methylthiotransferase RimO</fullName>
            <shortName evidence="1">S12 MTTase</shortName>
            <shortName evidence="1">S12 methylthiotransferase</shortName>
            <ecNumber evidence="1">2.8.4.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Ribosomal protein S12 (aspartate-C(3))-methylthiotransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Ribosome maturation factor RimO</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rimO</name>
        <name type="ordered locus">Rmet_1361</name>
    </gene>
    <organism>
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the methylthiolation of an aspartic acid residue of ribosomal protein S12.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>[sulfur carrier]-SH + AH2 + L-aspartate(89)-[ribosomal protein uS12]-hydrogen + 2 S-adenosyl-L-methionine = 3-methylsulfanyl-L-aspartate(89)-[ribosomal protein uS12]-hydrogen + 5'-deoxyadenosine + [sulfur carrier]-H + A + 2 H(+) + L-methionine + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:37087"/>
            <dbReference type="Rhea" id="RHEA-COMP:10460"/>
            <dbReference type="Rhea" id="RHEA-COMP:10461"/>
            <dbReference type="Rhea" id="RHEA-COMP:14737"/>
            <dbReference type="Rhea" id="RHEA-COMP:14739"/>
            <dbReference type="ChEBI" id="CHEBI:13193"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17319"/>
            <dbReference type="ChEBI" id="CHEBI:17499"/>
            <dbReference type="ChEBI" id="CHEBI:29917"/>
            <dbReference type="ChEBI" id="CHEBI:29961"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:64428"/>
            <dbReference type="ChEBI" id="CHEBI:73599"/>
            <dbReference type="EC" id="2.8.4.4"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="1">Binds 2 [4Fe-4S] clusters. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the methylthiotransferase family. RimO subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.8.4.4" evidence="1"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08244.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516124.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q1LNN2"/>
    <dbReference type="SMR" id="Q1LNN2"/>
    <dbReference type="STRING" id="266264.Rmet_1361"/>
    <dbReference type="EnsemblBacteria" id="ABF08244">
        <property type="protein sequence ID" value="ABF08244"/>
        <property type="gene ID" value="Rmet_1361"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1361"/>
    <dbReference type="eggNOG" id="COG0621">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018697_0_0_4"/>
    <dbReference type="OMA" id="HYAYPTG"/>
    <dbReference type="OrthoDB" id="397139at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0103039">
        <property type="term" value="F:protein methylthiotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018339">
        <property type="term" value="P:peptidyl-L-beta-methylthioaspartic acid biosynthetic process from peptidyl-aspartic acid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006400">
        <property type="term" value="P:tRNA modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12160">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.30.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01865">
        <property type="entry name" value="MTTase_RimO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaB/NifB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005839">
        <property type="entry name" value="Methylthiotransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020612">
        <property type="entry name" value="Methylthiotransferase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013848">
        <property type="entry name" value="Methylthiotransferase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038135">
        <property type="entry name" value="Methylthiotransferase_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005840">
        <property type="entry name" value="Ribosomal_S12_MeSTrfase_RimO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041582">
        <property type="entry name" value="RimO_TRAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023404">
        <property type="entry name" value="rSAM_horseshoe"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002792">
        <property type="entry name" value="TRAM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43837">
        <property type="entry name" value="PTHR43837"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18693">
        <property type="entry name" value="TRAM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00919">
        <property type="entry name" value="UPF0004"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00274">
        <property type="entry name" value="ribosomal_protein_S12_methylth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00089">
        <property type="entry name" value="TIGR00089"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01125">
        <property type="entry name" value="TIGR01125"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51449">
        <property type="entry name" value="MTTASE_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01278">
        <property type="entry name" value="MTTASE_RADICAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50926">
        <property type="entry name" value="TRAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0004">4Fe-4S</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0408">Iron</keyword>
    <keyword id="KW-0411">Iron-sulfur</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000374961" description="Ribosomal protein S12 methylthiotransferase RimO">
        <location>
            <begin position="1"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="domain" description="MTTase N-terminal" evidence="1">
        <location>
            <begin position="11"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="domain" description="Radical SAM core" evidence="2">
        <location>
            <begin position="143"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="domain" description="TRAM" evidence="1">
        <location>
            <begin position="387"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="20"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="56"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="85"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="157"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="161"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="164"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01266"/>
        </source>
    </evidence>
    <sequence length="456" mass="49741" checksum="4EC34E3CEE955F4A" modified="2006-05-30" version="1">MSSDTNLSNPPKVGFVSLGCPKALVDSEQIITQLRAEGYSISGTYDGADLVVVNTCGFIDEAVQESLDAIGEALTENGKVIVTGCLGAKKDAAGHDIVSAVHPKVLAVTGPHALGEVMQAVHTHLPKPHDPFTDLVPAAGIKLTPKHYAYLKISEGCNHRCSFCIIPSMRGDLVSRPVAEVMLEAENLFKAGVKELLVISQDTSAYGVDVKYRTGFWNGRPLKTRMTELVAALGELASQYGAWVRLHYVYPYPHVDEIIPLMNQGNVLPYLDVPLQHAHPDVLKRMKRPANAEKTLDRIRAWREVCPDLTIRSTFIAGFPGETEEEFQTLLDFIAEAELDRVGCFAYSPVEGATANDLPGALPDEVREERRARFMEVAEEVSARRLQRKVGQTLRVLVDEVNQDGGIGRSSADAPEIDGLVYIDPAAKASQRYKTGDFVNVKITGADGHDLWGEVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="68" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LNQ1</accession>
    <name>Q1LNQ1_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Conjugal transfer TrbC transmembrane protein (CMGI-2)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">trbC</name>
        <name evidence="2" type="ordered locus">Rmet_1342</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08225.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516107.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1342"/>
    <dbReference type="EnsemblBacteria" id="ABF08225">
        <property type="protein sequence ID" value="ABF08225"/>
        <property type="gene ID" value="Rmet_1342"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1342"/>
    <dbReference type="eggNOG" id="COG3838">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_143299_2_0_4"/>
    <dbReference type="OMA" id="MTISAFR"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007039">
        <property type="entry name" value="TrbC/VirB2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04956">
        <property type="entry name" value="TrbC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="72"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="101"/>
            <end position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF08225.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="127" mass="13143" checksum="331CE27F7FE37C7A" modified="2006-05-30" version="1">MTQMTISAFRVSVNPALRLARLRSLARPAGQGLLLAALLLFLAGTAQAAGSSMPWEGPLQSILESIQGPVARIVAVIIIIATGLALAFGDTSGGFRKLIQIVFGLSIAFAASSFFLSFFSFSGGAVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="108" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LP70</accession>
    <name>Q1LP70_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Cytochrome c biogenesis protein, transmembrane region</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Rmet_1170</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08056.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515951.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1170"/>
    <dbReference type="EnsemblBacteria" id="ABF08056">
        <property type="protein sequence ID" value="ABF08056"/>
        <property type="gene ID" value="Rmet_1170"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1170"/>
    <dbReference type="eggNOG" id="COG0526">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG0785">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033708_0_0_4"/>
    <dbReference type="OMA" id="GQIRYHH"/>
    <dbReference type="OrthoDB" id="1617952at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017004">
        <property type="term" value="P:cytochrome complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003834">
        <property type="entry name" value="Cyt_c_assmbl_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013740">
        <property type="entry name" value="Redoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041017">
        <property type="entry name" value="Thioredoxin_10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02683">
        <property type="entry name" value="DsbD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08534">
        <property type="entry name" value="Redoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17991">
        <property type="entry name" value="Thioredoxin_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="SSF52833"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="6"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="40"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="70"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="123"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="161"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="domain" description="Thioredoxin" evidence="7">
        <location>
            <begin position="267"/>
            <end position="418"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ABF08056.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="596" mass="63150" checksum="41FB4B239A9BB776" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LPC4</accession>
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            <fullName evidence="2">Ornithine cyclodeaminase (OCD)</fullName>
            <ecNumber evidence="2">4.3.1.12</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">ocd</name>
        <name evidence="2" type="ordered locus">Rmet_1116</name>
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        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EC" id="4.3.1.12" evidence="2"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08002.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515903.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1116"/>
    <dbReference type="EnsemblBacteria" id="ABF08002">
        <property type="protein sequence ID" value="ABF08002"/>
        <property type="gene ID" value="Rmet_1116"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1116"/>
    <dbReference type="eggNOG" id="COG2423">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_042088_3_1_4"/>
    <dbReference type="OMA" id="YRMAGAN"/>
    <dbReference type="OrthoDB" id="1193876at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008473">
        <property type="term" value="F:ornithine cyclodeaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016639">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-NH2 group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019290">
        <property type="term" value="P:siderophore biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1780.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003462">
        <property type="entry name" value="ODC_Mu_crystall"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023401">
        <property type="entry name" value="ODC_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023866">
        <property type="entry name" value="SbnB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13812">
        <property type="entry name" value="PTHR13812"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02423">
        <property type="entry name" value="OCD_Mu_crystall"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001439">
        <property type="entry name" value="CryM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03944">
        <property type="entry name" value="dehyd_SbnB_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0456">Lyase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF08002.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="355" mass="39022" checksum="AFDEC6BE2F80ECDA" modified="2006-05-30" version="1">MTNSPTLTATTLPSASNPSHTKPELRYLSRDDLIALGAGHSDAFVQAITEGLAIHARGEHVQPLKPYLRWSGADHIADRIIAMPCYIGGDNPIAGIKWIGSRQHNPARFGLERASAVIVLNDATTNYPVAIMEGGLISGMRTAAITAVATRHLARSGFTDVACIGCGPIAQMQMQTLLEQFPQIRAVHLFDLSRDAMHAMRSMLGSRFPAVGWELCGSAELAVRAADVVVTCTVTDTPYLEHAWLKTGAFVCNVSIMDVHKDVYEKVDKVIVDDWDQSNREKKIINQLVLEGRFSREQLHAELGEIVIGRKPGRERDDEIILLNPMGMALDDMVCARHFYHLALERNAGTRLPLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="69" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LPD4</accession>
    <name>Q1LPD4_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative membrane protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_1106</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07992.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515893.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1106"/>
    <dbReference type="EnsemblBacteria" id="ABF07992">
        <property type="protein sequence ID" value="ABF07992"/>
        <property type="gene ID" value="Rmet_1106"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1106"/>
    <dbReference type="eggNOG" id="COG3182">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025664_2_0_4"/>
    <dbReference type="OMA" id="HFDLHYM"/>
    <dbReference type="OrthoDB" id="1656356at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005625">
        <property type="entry name" value="PepSY-ass_TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34219">
        <property type="entry name" value="PTHR34219"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03929">
        <property type="entry name" value="PepSY_TM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="159"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="203"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="357"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="404"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="435"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="464"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="495"/>
            <end position="520"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF07992.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="542" mass="58816" checksum="FCFFE432BFC6153B" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="67" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LPI0</accession>
    <name>Q1LPI0_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_1060</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07946.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515848.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1060"/>
    <dbReference type="EnsemblBacteria" id="ABF07946">
        <property type="protein sequence ID" value="ABF07946"/>
        <property type="gene ID" value="Rmet_1060"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1060"/>
    <dbReference type="eggNOG" id="COG2833">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035354_0_1_4"/>
    <dbReference type="OMA" id="WRFAGMP"/>
    <dbReference type="OrthoDB" id="1291114at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007402">
        <property type="entry name" value="DUF455"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009078">
        <property type="entry name" value="Ferritin-like_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011197">
        <property type="entry name" value="UCP012318"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04305">
        <property type="entry name" value="DUF455"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF012318">
        <property type="entry name" value="UCP012318"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47240">
        <property type="entry name" value="SSF47240"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF07946.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="283" mass="30996" checksum="77FC37B9D8089E0C" modified="2006-05-30" version="1">MPDIAPAEIADSPLATRRRALAVLCITDARAKAAAARALYTDLQAHGGTVTDVDEILVAEGSAAIPGRPERPELVPPQQVDRRRSLHTLAGRAVMIHALCHIEFNAINLALDAVWRFAGMPEAYYRDWLRVADEEALHFTLLADHLATLGATYGDYPAHNSLWEMTDRTSGDVLARMALVPRTLEARGLDASPPVRAKLAEVGDTAAAEIIDIILRDEVGHVAIGNHWYRWLCAQRGLDPIATYARLAEQYRAPKLRGPFNLEARRAAGFDEDELAWLEASAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LPK2</accession>
    <name>Q1LPK2_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Single-stranded-DNA-specific exonuclease RecJ</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">recJ</name>
        <name evidence="9" type="ordered locus">Rmet_1038</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the RecJ family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07924.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515829.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1038"/>
    <dbReference type="EnsemblBacteria" id="ABF07924">
        <property type="protein sequence ID" value="ABF07924"/>
        <property type="gene ID" value="Rmet_1038"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1038"/>
    <dbReference type="eggNOG" id="COG0608">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009736_5_1_4"/>
    <dbReference type="OMA" id="FIICDHH"/>
    <dbReference type="OrthoDB" id="491634at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008409">
        <property type="term" value="F:5'-3' exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001667">
        <property type="entry name" value="DDH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038763">
        <property type="entry name" value="DHH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003156">
        <property type="entry name" value="DHHA1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004610">
        <property type="entry name" value="RecJ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041122">
        <property type="entry name" value="RecJ_OB"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01368">
        <property type="entry name" value="DHH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02272">
        <property type="entry name" value="DHHA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17768">
        <property type="entry name" value="RecJ_OB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64182">
        <property type="entry name" value="SSF64182"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00644">
        <property type="entry name" value="recJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="4 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0540">Nuclease</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DHH" evidence="6">
        <location>
            <begin position="74"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="domain" description="DHHA1" evidence="7">
        <location>
            <begin position="319"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="domain" description="RecJ_OB" evidence="8">
        <location>
            <begin position="468"/>
            <end position="563"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005915"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00019841"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01368"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF02272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF17768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ABF07924.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="566" mass="61280" checksum="4F35EF1AA258DE34" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="97" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LPQ7</accession>
    <name>Q1LPQ7_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Transcriptional regulator, LysR-family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_0983</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the LysR transcriptional regulatory family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07869.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515787.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0983"/>
    <dbReference type="EnsemblBacteria" id="ABF07869">
        <property type="protein sequence ID" value="ABF07869"/>
        <property type="gene ID" value="Rmet_0983"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0983"/>
    <dbReference type="eggNOG" id="COG0583">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039613_37_1_4"/>
    <dbReference type="OMA" id="ETFYMCI"/>
    <dbReference type="OrthoDB" id="846433at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005119">
        <property type="entry name" value="LysR_subst-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000847">
        <property type="entry name" value="Tscrpt_reg_HTH_LysR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00126">
        <property type="entry name" value="HTH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03466">
        <property type="entry name" value="LysR_substrate"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00039">
        <property type="entry name" value="HTHLYSR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50931">
        <property type="entry name" value="HTH_LYSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH lysR-type" evidence="2">
        <location>
            <begin position="6"/>
            <end position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF07869.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="300" mass="33031" checksum="E9E321511A990A6F" modified="2006-05-30" version="1">MRRLCPSLTELQAFEAAARHNSFTLAARELHVTQGAVSKQVRSLEAFLGVELFERVRQRLMLTGAGERYLERIRPSLNEIEAASVELIARKGRGGILHIASMPTLGAKWLIPRLPQFFARHPEVTLEFVPHAQGYDFSQPDLDAAIRFGDGVWPGSLADYMTGREVLPVCRPGLLAQESGGAVASAEGLLRYPLLHHTTVPEAWPDWLSELGVSTRQAWSGARFDQFSLLTQAAVSGLGIALIPRCLIEEEIATGTLVVAHAARVLAKKGYYLCYPEQKAHLPALQTFRAWLMEGVDVVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="59" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LPX1</accession>
    <name>Q1LPX1_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Lipoprotein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_0919</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07805.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515742.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0919"/>
    <dbReference type="EnsemblBacteria" id="ABF07805">
        <property type="protein sequence ID" value="ABF07805"/>
        <property type="gene ID" value="Rmet_0919"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0919"/>
    <dbReference type="eggNOG" id="COG2913">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_090266_1_1_4"/>
    <dbReference type="OMA" id="GVWPSLM"/>
    <dbReference type="OrthoDB" id="1899049at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF07805.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="177" mass="20611" checksum="34B80671AEBA183E" modified="2006-05-30" version="1">MTSRYQRGSRHLLQILWAVLATLALGACTVLAPVDSGLKLIGQPQSAVQAAFGKPTDVYKLDDGTTRWFYSKQPLGEYSYAADFDRDDKLTNFRQMLTTTELYKAKVNAWTKKDVLEHFGAPREPIEYYPRLKQEAWSYRFLHEDQWYSMFNFMFDDTGVLRQTLVTPDPLRDRPER</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LQ17</accession>
    <name>Q1LQ17_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative transcriptional regulator, LuxR family (Domain CheY-like)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_0873</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07759.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515700.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0873"/>
    <dbReference type="EnsemblBacteria" id="ABF07759">
        <property type="protein sequence ID" value="ABF07759"/>
        <property type="gene ID" value="Rmet_0873"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0873"/>
    <dbReference type="eggNOG" id="COG2197">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1863542_0_0_4"/>
    <dbReference type="OrthoDB" id="1687028at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Response regulatory" evidence="2">
        <location>
            <begin position="2"/>
            <end position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF07759.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="137" mass="15343" checksum="453D09E1F08D8F83" modified="2006-05-30" version="1">MGAIVIESHPLTRLGMQRLLERMPGIGPVESIDPEGILLFAHSLEDVLVIYGMTVDAFDNWYLLRRLHQALPNARILLLSDNIWMHVPSSLRACGVQEHLPKSASIERMEAVLMRMLGDPDVAVNALMDDSMFGSHR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="65" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LQ35</accession>
    <name>Q1LQ35_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized stress-induced protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Rmet_0855</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07741.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008647044.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0855"/>
    <dbReference type="EnsemblBacteria" id="ABF07741">
        <property type="protein sequence ID" value="ABF07741"/>
        <property type="gene ID" value="Rmet_0855"/>
    </dbReference>
    <dbReference type="GeneID" id="60825339"/>
    <dbReference type="KEGG" id="rme:Rmet_0855"/>
    <dbReference type="eggNOG" id="COG1561">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_076609_2_0_4"/>
    <dbReference type="OMA" id="EIKSVNH"/>
    <dbReference type="OrthoDB" id="1509828at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005229">
        <property type="entry name" value="CHP00255"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013551">
        <property type="entry name" value="DUF1732"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013527">
        <property type="entry name" value="YicC-like_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30636">
        <property type="entry name" value="PTHR30636"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08340">
        <property type="entry name" value="DUF1732"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03755">
        <property type="entry name" value="YicC_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00255">
        <property type="entry name" value="TIGR00255"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="YicC_N" evidence="2">
        <location>
            <begin position="2"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="domain" description="DUF1732" evidence="3">
        <location>
            <begin position="227"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="282"/>
            <end position="311"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF03755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF08340"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF07741.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="311" mass="34582" checksum="97AA429DD7FC7E15" modified="2006-05-30" version="1">MIHSMTGYGLATGQAPLTNAQGEPSGRTASVSVEFRTVNSRFLDLLFRAPEECRAFEPALREMLMGELSRGKLECRINLQRTDTGGATLALNQTLLDQIRALEATVGATFASAGTMRMGEILRWPGVLVEPELSQEALRDAVMGAAREALNQLLEARRREGAALKATLEERIDAMLAIVERLTPMIPQLIAHHQEKLTERLKEAFNLVAPNGMPSMSRDEIAERIRQEATVYGIRIDIAEELARLQTHLNETRHILKKGGQVGKRLDFMMQELNREANTLGSKAAAKELADASMELKLLIEQMREQIQNLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="81" xmlns="http://uniprot.org/uniprot">
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    <name>Q1LQ95_CUPMC</name>
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        </submittedName>
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    <organism evidence="7 8">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
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        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07681.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0795"/>
    <dbReference type="EnsemblBacteria" id="ABF07681">
        <property type="protein sequence ID" value="ABF07681"/>
        <property type="gene ID" value="Rmet_0795"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0795"/>
    <dbReference type="eggNOG" id="COG1079">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_040769_1_1_4"/>
    <dbReference type="OMA" id="MIFGNWM"/>
    <dbReference type="OrthoDB" id="1215743at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001851">
        <property type="entry name" value="ABC_transp_permease"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02653">
        <property type="entry name" value="BPD_transp_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="33"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="60"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="92"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="135"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="184"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="212"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="238"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="265"/>
            <end position="283"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABF07681.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="306" mass="32707" checksum="70DC17BDF1D0BB0E" modified="2006-05-30" version="1">MEQLAPLIATAINAGTPLLLAALGLLINERAGVLNLGAEGMMLVAAAFGFMVGYQTQSPMLGFVAGAVAGMLMATLFSWLALVLATNQVATGLALSIFGTGLSAFIAQRFVGYAMPAQAKSVPYLSDLPFVGPAFFQHHWMVYFSLALCFAIMWFLFRTRAGLTLRAIGESPESAHALGYPVRWIRFCALLFGGACCGLAGAYLSLVYTPMWVENLVAGRGWIALALTTFATWRPGRVLIGAWLFGGVTILQFYLQGIGVSVPSQFLSMLPYAATIVVLALISRNPAWIRLNMPASLGKPFRPGNA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="64" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LQK8</accession>
    <name>Q1LQK8_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Putative membrane protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">Rmet_0682</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
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        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the UPF0126 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07568.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0682"/>
    <dbReference type="EnsemblBacteria" id="ABF07568">
        <property type="protein sequence ID" value="ABF07568"/>
        <property type="gene ID" value="Rmet_0682"/>
    </dbReference>
    <dbReference type="GeneID" id="60825160"/>
    <dbReference type="KEGG" id="rme:Rmet_0682"/>
    <dbReference type="eggNOG" id="COG2860">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_064906_2_0_4"/>
    <dbReference type="OMA" id="RAPRAWN"/>
    <dbReference type="OrthoDB" id="1665397at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005115">
        <property type="entry name" value="Gly_transporter"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03458">
        <property type="entry name" value="Gly_transporter"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="16"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="42"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="78"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="108"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
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            <end position="153"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="165"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="190"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="domain" description="Gly_transporter" evidence="8">
        <location>
            <begin position="20"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="domain" description="Gly_transporter" evidence="8">
        <location>
            <begin position="108"/>
            <end position="181"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF03458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ABF07568.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="215" mass="23453" checksum="DD63DBC62972916C" modified="2006-05-30" version="1">MQYHLPFDLLMGRLPLILHVMEVIGVLAFAVSGVVDARKQRLDVVGTFVVAFATAFGGGTLRDVLLDRRPFYWVDHEYYVLLIFVMSLGASFVLRVASRVASDRTMLVADAIGLGLFSVTGTSLALVAQMTPTVAVMMGIISAVFGGVVRDVLCNEVPMILRDRSPYATCSFVGCWIYVGLTWLRADQEVALLAGALVTMGMRLVSVQFGWKLPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="66" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LQM8</accession>
    <name>Q1LQM8_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative integral membrane prtein with MHYT domain</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_0662</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07548.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515519.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0662"/>
    <dbReference type="EnsemblBacteria" id="ABF07548">
        <property type="protein sequence ID" value="ABF07548"/>
        <property type="gene ID" value="Rmet_0662"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0662"/>
    <dbReference type="eggNOG" id="COG3300">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_061170_1_0_4"/>
    <dbReference type="OMA" id="VMGVAVC"/>
    <dbReference type="OrthoDB" id="1290978at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005330">
        <property type="entry name" value="MHYT_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03707">
        <property type="entry name" value="MHYT"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50924">
        <property type="entry name" value="MHYT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="17"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="58"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="86"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="119"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="150"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="185"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="225"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="domain" description="MHYT" evidence="2">
        <location>
            <begin position="17"/>
            <end position="207"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50924"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF07548.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="262" mass="27234" checksum="62732447E1654DC1" modified="2006-05-30" version="1">MFSGFAAVPGAVVPYSYNWLMVGLSFLISACGAYVALRWCRGIRKPNGRLDVDRLMCASVALGGGAVWAMHFIGMVAYQTPTHMEFSLLVTLASLLAVMVFAAAGLAKASSPTGNPLSNTIKGGVLTGTGVVVMHYTGMAAIHTNSVFEWDLFIVALSALIAIVVSAVGLWLATNVKTVAQQIGAALVMAVAVCGMHYTGMTAGTMICTGRTYSPTLLALEGDNMGYAVFALTAVVLIFILVIEATRSGSQSVSVASASGRH</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LQP8</accession>
    <name>Q1LQP8_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Flp pilus assembly ATPase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">cpaE1</name>
        <name evidence="4" type="ordered locus">Rmet_0642</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07528.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515499.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0642"/>
    <dbReference type="EnsemblBacteria" id="ABF07528">
        <property type="protein sequence ID" value="ABF07528"/>
        <property type="gene ID" value="Rmet_0642"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0642"/>
    <dbReference type="eggNOG" id="COG4565">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG4963">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033160_2_0_4"/>
    <dbReference type="OMA" id="PRATMEE"/>
    <dbReference type="OrthoDB" id="1370117at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025669">
        <property type="entry name" value="AAA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13614">
        <property type="entry name" value="AAA_31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Response regulatory" evidence="3">
        <location>
            <begin position="3"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="370"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="1">
        <location>
            <position position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF07528.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="397" mass="43828" checksum="035B75D5D6BD5433" modified="2006-05-30" version="1">MLKILIASDDTARLAEINRLATIAGNFQTTSLQEGLARLPFQASRLRMADLLVLDLSEIGPAQMFGVETLRQQHPELPCILITQSQDSSVLIQAMRAGVRDVLPWPLDKGQLVEALKRVEATHVPRMQEVAQVISMISCKGGAGTSFVAANLGDALARHFGKRVLVVDLNRYFGDLTYIVTDKAPPSTLSEICSQIDRMDAAFLEACLVHVDNGFDMLAGAADPVRASQIQKEKLEWILSVMQPNYDYVIFDVGQSIDPLSISMLDHSDRICVVTEPSIAFGRPSRRLLDILRALHYPDDKIRVVLNRVGRKNEVPRTTMEEIFGMKVAFTLPDDPAAVDEAVSHGEPVAKLSKRSSIARALQAMAMQLAPSHEAERRGRQEGVSPLRKLMLRARST</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LRC1</accession>
    <name>Q1LRC1_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Phospholipid-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">ybhB</name>
        <name evidence="1" type="ordered locus">Rmet_0419</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07305.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515294.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0419"/>
    <dbReference type="EnsemblBacteria" id="ABF07305">
        <property type="protein sequence ID" value="ABF07305"/>
        <property type="gene ID" value="Rmet_0419"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0419"/>
    <dbReference type="eggNOG" id="COG1881">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_083918_0_0_4"/>
    <dbReference type="OMA" id="CPPWNDS"/>
    <dbReference type="OrthoDB" id="1150223at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd00865">
        <property type="entry name" value="PEBP_bact_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.280.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008914">
        <property type="entry name" value="PEBP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036610">
        <property type="entry name" value="PEBP-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005247">
        <property type="entry name" value="YbhB_YbcL"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30289">
        <property type="entry name" value="PTHR30289"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01161">
        <property type="entry name" value="PBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49777">
        <property type="entry name" value="SSF49777"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00481">
        <property type="entry name" value="TIGR00481"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF07305.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="215" mass="23347" checksum="997B05208653570E" modified="2006-05-30" version="1">MKLWSKAFADNAPIPGEFAFCVPDSASHVALSSNRNPDLHWDDVPAATRSFVLICHDRDVPSKGDDVNQEGREVPESLPRVDFFHWVLIDVPPGLHTISAGSHSDGVIARGKPGPEALVGTATAGGLRHGLNDYTGWFAGDADMKGDYFGYDGPCPPWNDSIIHHYVFTVYALDIDRVPVEGKFTGADVRRAIQGHVLAEASLTGTYTLNPRLVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-10-07" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LRD1</accession>
    <name>Q1LRD1_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_0409</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07295.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF07295">
        <property type="protein sequence ID" value="ABF07295"/>
        <property type="gene ID" value="Rmet_0409"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0409"/>
    <dbReference type="HOGENOM" id="CLU_2119071_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF07295.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="114" mass="12788" checksum="7FFDEEFE1CE28777" modified="2006-05-30" version="1">MWLFVKMAGGQQRAIELDGQPASRFRWAALAKDEKKPKSVGLGFESTKGGGWRRHRAIDGAGLGGRCDERFGYVTDFRFRTLARSHAPSFDGLNYTYRHSRCKKICSAKCDTAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="65" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LRG4</accession>
    <name>Q1LRG4_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">CxxC_CXXC_SSSS domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_0376</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07262.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515254.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0376"/>
    <dbReference type="EnsemblBacteria" id="ABF07262">
        <property type="protein sequence ID" value="ABF07262"/>
        <property type="gene ID" value="Rmet_0376"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0376"/>
    <dbReference type="eggNOG" id="COG2331">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_136025_0_0_4"/>
    <dbReference type="OMA" id="TYQYQCT"/>
    <dbReference type="OrthoDB" id="1914830at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013429">
        <property type="entry name" value="Regulatory_FmdB_Zinc_ribbon"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09723">
        <property type="entry name" value="Zn-ribbon_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00834">
        <property type="entry name" value="CxxC_CXXC_SSSS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02605">
        <property type="entry name" value="CxxC_CxxC_SSSS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CxxC_CXXC_SSSS" evidence="2">
        <location>
            <begin position="1"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="63"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="64"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00834"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF07262.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="109" mass="10655" checksum="1439CF81B9E544C4" modified="2006-05-30" version="1">MPIYAYRCDACGYGRDVLQKMSDAPLTDCPSCGAAGTYKKQLTAAGFQLKGSGWYVTDFRGGSGGTSAPATSGNATAPAAPAASAESSTASTTSAAPAAGGCGDSCACH</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LRT4</accession>
    <name>Q1LRT4_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Extra-cytoplasmic Solute Receptor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">bug</name>
        <name evidence="4" type="ordered locus">Rmet_0256</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Periplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the UPF0065 (bug) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07142.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515149.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0256"/>
    <dbReference type="EnsemblBacteria" id="ABF07142">
        <property type="protein sequence ID" value="ABF07142"/>
        <property type="gene ID" value="Rmet_0256"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0256"/>
    <dbReference type="eggNOG" id="COG3181">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045683_0_0_4"/>
    <dbReference type="OMA" id="QAGFMVP"/>
    <dbReference type="OrthoDB" id="447734at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042597">
        <property type="term" value="C:periplasmic space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005064">
        <property type="entry name" value="BUG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042100">
        <property type="entry name" value="Bug_dom1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006311">
        <property type="entry name" value="TAT_signal"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42928">
        <property type="entry name" value="PTHR42928"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03401">
        <property type="entry name" value="TctC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017082">
        <property type="entry name" value="YflP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51318">
        <property type="entry name" value="TAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0675">Receptor</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193475" evidence="3">
        <location>
            <begin position="27"/>
            <end position="327"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF07142.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="327" mass="34439" checksum="86992DCD395BB98F" modified="2006-05-30" version="1">MPSRRHTLKALIATFAAGAVPAVAHADTWPAKPIRMIVPFPPGSSPDLIARIVTEKLATVLGQPVVVENRPGAGGNIGTGMVAKAAPDGYTLLFTINGPLVTAPSLYRHLSYDPMKQLAPVTLVATSPNVLVIDARLPVHTLREFVALAKSKPGALNYGSVGNGSAAHLAMEQLKAMAGIDLQHVPYPGFPQITTAIVGGQVQAGFMVPAIAMPMVNAGKLRILAVTTTGRTSLLPSVPTVAESGYPGFEAISWQAILAPAGTPAPVVDRLYRELVRIIGSDDIREKMRTQYFVPAGTAPASLRETMVSEKARWDKVIRAAGVQPEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="97" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LRX8</accession>
    <name>Q1LRX8_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">3-hydroxybutyryl-CoA dehydrogenase</fullName>
            <ecNumber evidence="8">1.1.1.157</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">hbdA</name>
        <name evidence="8" type="ordered locus">Rmet_0212</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the 3-hydroxyacyl-CoA dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.157" evidence="8"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07098.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515117.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0212"/>
    <dbReference type="EnsemblBacteria" id="ABF07098">
        <property type="protein sequence ID" value="ABF07098"/>
        <property type="gene ID" value="Rmet_0212"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0212"/>
    <dbReference type="eggNOG" id="COG1250">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009834_2_0_4"/>
    <dbReference type="OMA" id="SVMEVFY"/>
    <dbReference type="OrthoDB" id="862072at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008691">
        <property type="term" value="F:3-hydroxybutyryl-CoA dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070403">
        <property type="term" value="F:NAD+ binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044248">
        <property type="term" value="P:cellular catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006631">
        <property type="term" value="P:fatty acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901575">
        <property type="term" value="P:organic substance catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1040.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022694">
        <property type="entry name" value="3-OHacyl-CoA_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006180">
        <property type="entry name" value="3-OHacyl-CoA_DH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006176">
        <property type="entry name" value="3-OHacyl-CoA_DH_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006108">
        <property type="entry name" value="3HC_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008927">
        <property type="entry name" value="6-PGluconate_DH-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013328">
        <property type="entry name" value="6PGD_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00725">
        <property type="entry name" value="3HCDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02737">
        <property type="entry name" value="3HCDH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000105">
        <property type="entry name" value="HCDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48179">
        <property type="entry name" value="SSF48179"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00067">
        <property type="entry name" value="3HCDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0520">NAD</keyword>
    <keyword evidence="8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="3HCDH_N" evidence="7">
        <location>
            <begin position="5"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="domain" description="3HCDH" evidence="6">
        <location>
            <begin position="186"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="10"/>
            <end position="15"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="33"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="49"/>
        </location>
        <ligand>
            <name>CoA</name>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="56"/>
        </location>
        <ligand>
            <name>CoA</name>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="92"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="97"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4 5">
        <location>
            <position position="119"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="143"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="274"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="site" description="Important for catalytic activity" evidence="3">
        <location>
            <position position="140"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009463"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR000105-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR000105-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR000105-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF02737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ABF07098.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="284" mass="30199" checksum="AED14A8B0D4E5E98" modified="2006-05-30" version="1">MAIKTVGIVGAGTMGNGIAQACAVVGIDVVMIDISDAAVQKGIATVAGSLDRLIKKEKATEADKAAALARIHGSTSYDDLKRADIVIEAATENFDLKVKILKQIDGIVGPDVIVASNTSSISITKLAAVTSRADRFIGMHFFNPVPMMALVELIRGLQTSDAAHADVEDLAKRLGKYPITVKNSPGFVVNRILCPMINEAFCVLGEGLASPEEIDEGMKLGCNHPIGPLALADMIGLDTMLAVMEVLYTEFADPKYRPAMLMREMVAAGYLGRKTGRGVYVYSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="97" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LS31</accession>
    <name>Q1LS31_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Choline dehydrogenase, a flavoprotein</fullName>
            <ecNumber evidence="4">1.1.99.1</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">betA1</name>
        <name evidence="4" type="ordered locus">Rmet_0159</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the GMC oxidoreductase family.</text>
    </comment>
    <dbReference type="EC" id="1.1.99.1" evidence="4"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07045.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515071.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0159"/>
    <dbReference type="EnsemblBacteria" id="ABF07045">
        <property type="protein sequence ID" value="ABF07045"/>
        <property type="gene ID" value="Rmet_0159"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0159"/>
    <dbReference type="eggNOG" id="COG2303">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002865_7_1_4"/>
    <dbReference type="OMA" id="GWGWQDV"/>
    <dbReference type="OrthoDB" id="543793at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008812">
        <property type="term" value="F:choline dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012132">
        <property type="entry name" value="GMC_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000172">
        <property type="entry name" value="GMC_OxRdtase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007867">
        <property type="entry name" value="GMC_OxRtase_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11552">
        <property type="entry name" value="PTHR11552"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05199">
        <property type="entry name" value="GMC_oxred_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00732">
        <property type="entry name" value="GMC_oxred_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000137">
        <property type="entry name" value="Alcohol_oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="SSF51905"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00624">
        <property type="entry name" value="GMC_OXRED_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0274">FAD</keyword>
    <keyword evidence="2" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GMC_OxRdtase_N" evidence="3">
        <location>
            <begin position="267"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="232"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010790"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR000137-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS00624"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF07045.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="560" mass="60276" checksum="026B1A31FA2D5604" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="72" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LS71</accession>
    <name>Q1LS71_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">tRNA_edit domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_0119</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07005.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008642855.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0119"/>
    <dbReference type="EnsemblBacteria" id="ABF07005">
        <property type="protein sequence ID" value="ABF07005"/>
        <property type="gene ID" value="Rmet_0119"/>
    </dbReference>
    <dbReference type="GeneID" id="60822841"/>
    <dbReference type="KEGG" id="rme:Rmet_0119"/>
    <dbReference type="eggNOG" id="COG2606">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_094875_0_0_4"/>
    <dbReference type="OMA" id="VMLPATG"/>
    <dbReference type="OrthoDB" id="1935578at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002161">
        <property type="term" value="F:aminoacyl-tRNA editing activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.960.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036754">
        <property type="entry name" value="YbaK/aa-tRNA-synt-asso_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007214">
        <property type="entry name" value="YbaK/aa-tRNA-synth-assoc-dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04073">
        <property type="entry name" value="tRNA_edit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55826">
        <property type="entry name" value="SSF55826"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="tRNA_edit" evidence="1">
        <location>
            <begin position="34"/>
            <end position="149"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF04073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF07005.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="165" mass="17028" checksum="2897DDDCA4AFDEEB" modified="2006-05-30" version="1">MSESPTLPDAAQRVADLLVGIGHDRPVVMLPATGKTSAEAAAGLGCTVAEIAKSIIFRRVEDDVPVLVIASGSNRVDEAKVAARVGALGKADAKFVREKTGYAIGGVCPIGHAVAPVMLLDQDLFQYDSVWAAAGHPHAVFNLTPQQLQAMTGAEVADVAQVTSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LSD8</accession>
    <name>Q1LSD8_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Cell shape-determining protein MreC</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">Cell shape protein MreC</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">mreC</name>
        <name evidence="8" type="ordered locus">Rmet_0052</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the MreC family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF06938.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011514978.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0052"/>
    <dbReference type="EnsemblBacteria" id="ABF06938">
        <property type="protein sequence ID" value="ABF06938"/>
        <property type="gene ID" value="Rmet_0052"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0052"/>
    <dbReference type="eggNOG" id="COG1792">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_042663_2_0_4"/>
    <dbReference type="OMA" id="NPFTHRI"/>
    <dbReference type="OrthoDB" id="837894at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008360">
        <property type="term" value="P:regulation of cell shape"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.340">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.350">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042177">
        <property type="entry name" value="Cell/Rod_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042175">
        <property type="entry name" value="Cell/Rod_MreC_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007221">
        <property type="entry name" value="MreC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34138">
        <property type="entry name" value="PTHR34138"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04085">
        <property type="entry name" value="MreC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00219">
        <property type="entry name" value="mreC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0133">Cell shape</keyword>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="15"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="280"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="89"/>
            <end position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009369"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013855"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00032089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ABF06938.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="336" mass="36349" checksum="089A8D5FC1142A0E" modified="2006-05-30" version="1">MDYSPPPLFKQGTSAVARLVIFVTIALVLLIVDARFDAMRNVRQVAATVLMPVERLVLVPRDAIRGVFDYAQSSVELANENRELRRAALEQANASVRQAQLESENNQLRKLLNLTQQSTTPVTPAEILYDARDPYSQRIVIDRGSMQGLRAGYPVIDERGVIGQVTRVSPFQSEVTLLTDKDQAIPVQVVRNGLRSVAFGGSRAGLLDLRFMAASADLQQGDLLVTSGLDGTYPPGLPVAKIVQIERKADTAFSRVYCEPVAGVRSHRQLLVVRYESGLAPRPPEAATSQKAERGSRSAAARAGAEQKADKAATSADKPADTPTAAPAQKPSEPAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-04-26" modified="2022-01-19" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>Q58AF9</accession>
    <name>Q58AF9_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 6">Flagellar basal body rod protein FlgB</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">flgB</name>
        <name evidence="8" type="ordered locus">Rmet_6142</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="8 9">
        <name>pMOL30</name>
    </geneLocation>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="9">Plasmid pMOL30</plasmid>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5 6">Structural component of flagellum, the bacterial motility apparatus. Part of the rod structure of flagellar basal body.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">The basal body constitutes a major portion of the flagellar organelle and consists of a number of rings mounted on a central rod.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Bacterial flagellum basal body</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the flagella basal body rod proteins family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000354">
        <property type="protein sequence ID" value="ABF13001.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008642701.1">
        <property type="nucleotide sequence ID" value="NC_007971.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_145659.1">
        <property type="nucleotide sequence ID" value="NC_006466.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF13001">
        <property type="protein sequence ID" value="ABF13001"/>
        <property type="gene ID" value="Rmet_6142"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6142"/>
    <dbReference type="HOGENOM" id="CLU_125463_1_0_4"/>
    <dbReference type="OMA" id="NAMDFQT"/>
    <dbReference type="OrthoDB" id="1914857at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid pMOL30"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030694">
        <property type="term" value="C:bacterial-type flagellum basal body, rod"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071973">
        <property type="term" value="P:bacterial-type flagellum-dependent cell motility"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001444">
        <property type="entry name" value="Flag_bb_rod_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006300">
        <property type="entry name" value="FlgB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30435:SF12">
        <property type="entry name" value="PTHR30435:SF12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00460">
        <property type="entry name" value="Flg_bb_rod"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002889">
        <property type="entry name" value="Rod_FlgB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01396">
        <property type="entry name" value="FlgB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0975">Bacterial flagellum</keyword>
    <keyword evidence="8" id="KW-0966">Cell projection</keyword>
    <keyword evidence="8" id="KW-0969">Cilium</keyword>
    <keyword evidence="8" id="KW-0282">Flagellum</keyword>
    <keyword evidence="8" id="KW-0614">Plasmid</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Flg_bb_rod" evidence="7">
        <location>
            <begin position="19"/>
            <end position="44"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00014376"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023143"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00024934"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR002889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ABF13001.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="140" mass="15731" checksum="0ACF6A10FED50490" modified="2005-04-26" version="1">METAFIQNAARSAENAHELALRLRTRRLEFLAANIANADTPNYKARDIDFGAELERAMASGNAFVGMATTSRRHLESRPSAAEEALLYRVPMQSGIDGNTVEMDVERVAFAENAVRMRFSIQKTAGEYKDMLKLYQDMRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-04-26" modified="2022-05-25" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>Q58AN4</accession>
    <name>Q58AN4_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Alkylhydroperoxidase AhpD core</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">ahpD</name>
        <name evidence="2" type="ordered locus">Rmet_5993</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2 3">
        <name>pMOL30</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid pMOL30</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000354">
        <property type="protein sequence ID" value="ABF12852.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011229335.1">
        <property type="nucleotide sequence ID" value="NC_007971.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_145581.1">
        <property type="nucleotide sequence ID" value="NC_006466.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF12852">
        <property type="protein sequence ID" value="ABF12852"/>
        <property type="gene ID" value="Rmet_5993"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5993"/>
    <dbReference type="HOGENOM" id="CLU_137228_2_0_4"/>
    <dbReference type="OMA" id="VGEMWAL"/>
    <dbReference type="OrthoDB" id="1925754at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid pMOL30"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004601">
        <property type="term" value="F:peroxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051920">
        <property type="term" value="F:peroxiredoxin activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1290.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029032">
        <property type="entry name" value="AhpD-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003779">
        <property type="entry name" value="CMD-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02627">
        <property type="entry name" value="CMD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69118">
        <property type="entry name" value="SSF69118"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="2" id="KW-0575">Peroxidase</keyword>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CMD" evidence="1">
        <location>
            <begin position="27"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02627"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF12852.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="124" mass="13470" checksum="557988DB1E1390B3" modified="2005-04-26" version="1">MSQIDIDPALGLLAEFGKLTGNTTRHIKQMRQEAIFKDGALPARVKALSAMLWAISARCEPCFKFYVQQAVKLGATEMEMGEVLAVASTMGGCVGEMWALKAFKAYKDFTSGEAVPETEPSCCQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-01-04" modified="2021-09-29" version="92" xmlns="http://uniprot.org/uniprot">
    <accession>Q5NUY5</accession>
    <name>Q5NUY5_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">ParB involved in chromosome partitioning</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">parB</name>
        <name evidence="3" type="ordered locus">Rmet_6316</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3 4">
        <name>pMOL28</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid pMOL28</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000355">
        <property type="protein sequence ID" value="ABF13175.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008649477.1">
        <property type="nucleotide sequence ID" value="NC_007972.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_161626.1">
        <property type="nucleotide sequence ID" value="NC_006525.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF13175">
        <property type="protein sequence ID" value="ABF13175"/>
        <property type="gene ID" value="Rmet_6316"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6316"/>
    <dbReference type="HOGENOM" id="CLU_072315_0_0_4"/>
    <dbReference type="OMA" id="IRPNPWQ"/>
    <dbReference type="OrthoDB" id="653368at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid pMOL28"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003115">
        <property type="entry name" value="ParB/Sulfiredoxin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036086">
        <property type="entry name" value="ParB/Sulfiredoxin_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02195">
        <property type="entry name" value="ParBc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00470">
        <property type="entry name" value="ParB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110849">
        <property type="entry name" value="SSF110849"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ParB" evidence="2">
        <location>
            <begin position="70"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="39"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00470"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF13175.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="332" mass="36524" checksum="1C396564C2E64EA4" modified="2005-01-04" version="1">MSLREKLAAKAGNIKVTAEDLEKAAARGPQAPRTAPGQLMHMQGKVERQANEIAQLRAELESARVSGGAVDVPIDQLHEVPGRRRFMPPEKYVELRENLRHNKLVHPVIVCPRPAGGFEIVSGHHRTDAYRELGRDHIRCVLGELSSDEADTGAFYANLMQSDLTDFEKFRKFDELLLRSPDKTQAAIAEQAGVPVSTLSEILSFRNLPPEVLSLLDSRPDLLGSNAGAELARATKDGRGDRVVEAVKLLAEKKIDQQQAVRMTKAEQVKTRPAASTGFKIKAGKATWCDVRIAKKVMRIEFRSEEEAEAAQSAIREHLEGLAKAASEDAKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-03-23" modified="2020-10-07" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>D3DXQ6</accession>
    <name>D3DXQ6_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_6464</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ADC45076.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADC45076">
        <property type="protein sequence ID" value="ADC45076"/>
        <property type="gene ID" value="Rmet_6464"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6464"/>
    <dbReference type="eggNOG" id="COG3547">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2555803_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004803">
        <property type="term" value="F:transposase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006313">
        <property type="term" value="P:transposition, DNA-mediated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003346">
        <property type="entry name" value="Transposase_20"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02371">
        <property type="entry name" value="Transposase_20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADC45076.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="82" mass="9232" checksum="E80C694A1822D9F5" modified="2010-03-23" version="1">MPGIGPVLATTIMLGTGTISRFTEVGDFPSYCRCVDSRRESNRRKKGEVDTKDGNKYLAWAFVKAASFAMCLCLEARRFFER</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-03-23" modified="2021-09-29" version="49" xmlns="http://uniprot.org/uniprot">
    <accession>D3DXU7</accession>
    <name>D3DXU7_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">HTH cro/C1-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_6509</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ADC45117.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADC45117">
        <property type="protein sequence ID" value="ADC45117"/>
        <property type="gene ID" value="Rmet_6509"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6509"/>
    <dbReference type="eggNOG" id="COG1396">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_066192_10_2_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00093">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001387">
        <property type="entry name" value="Cro/C1-type_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010982">
        <property type="entry name" value="Lambda_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01381">
        <property type="entry name" value="HTH_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47413">
        <property type="entry name" value="SSF47413"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50943">
        <property type="entry name" value="HTH_CROC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH cro/C1-type" evidence="1">
        <location>
            <begin position="14"/>
            <end position="43"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADC45117.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="86" mass="9781" checksum="9697CB0168A731EB" modified="2010-03-23" version="1">MEIGLDETIASARISRYESGVHQPAVEIAQKLAKVLKVPTAYLYCDNDELAEFLLSWPYLIKADRKEIERIVENRLFSKGLLGKQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-03-23" modified="2020-10-07" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>D3DXY6</accession>
    <name>D3DXY6_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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            <taxon>Cupriavidus</taxon>
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    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ADC45156.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_6549"/>
    <dbReference type="EnsemblBacteria" id="ADC45156">
        <property type="protein sequence ID" value="ADC45156"/>
        <property type="gene ID" value="Rmet_6549"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6549"/>
    <dbReference type="HOGENOM" id="CLU_3256804_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADC45156.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="42" mass="4529" checksum="525EA6EF134C253B" modified="2010-03-23" version="1">MIVAAIARYLRGSVSGFGSQSIMVFNPDISRLVVASNMAKDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-03-23" modified="2021-06-02" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>D3DY16</accession>
    <name>D3DY16_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_6585</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ADC45186.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADC45186">
        <property type="protein sequence ID" value="ADC45186"/>
        <property type="gene ID" value="Rmet_6585"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6585"/>
    <dbReference type="HOGENOM" id="CLU_2828121_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="19"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="31"/>
            <end position="45"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADC45186.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="66" mass="7561" checksum="D009A10FE816C74D" modified="2010-03-23" version="1">MDESGGSAESVMASMRERWQRGKMTEATGGIQRLRQPSTIRPSLPLSPRIQAKRRHHNEAKRFWLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-03-23" modified="2022-08-03" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>D3DY22</accession>
    <name>D3DY22_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_6591</name>
        <name evidence="3" type="ordered locus">Rmet_6615</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid</name>
    </geneLocation>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="submission" date="2006-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of the chromosome of Ralstonia metallidurans CH34.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Copeland A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Barry K."/>
                <person name="Detter J.C."/>
                <person name="Glavina del Rio T."/>
                <person name="Hammon N."/>
                <person name="Israni S."/>
                <person name="Dalin E."/>
                <person name="Tice H."/>
                <person name="Martinez M."/>
                <person name="Goltsman E."/>
                <person name="Pitluck S."/>
                <person name="Schmutz J."/>
                <person name="Larimer F."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Kim E."/>
                <person name="Mergeay M."/>
                <person name="Benotmane M.A."/>
                <person name="Vallaeys T."/>
                <person name="Michaux A."/>
                <person name="Monchy S."/>
                <person name="Dunn J."/>
                <person name="McCorkle S."/>
                <person name="Taghavi S."/>
                <person name="van der Lelie D."/>
                <person name="Richardson P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CH34</strain>
            <plasmid evidence="3">megaplasmid</plasmid>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2010-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Benotmane R."/>
                <person name="Lapidus A."/>
                <person name="McCorkle S."/>
                <person name="Monchy S."/>
                <person name="Taghavi S."/>
                <person name="van der Lelie N."/>
                <person name="Dunn J."/>
                <person name="Leys N."/>
                <person name="Mergeay M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">CH34</strain>
            <plasmid evidence="3">megaplasmid</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ADC45192.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ADC45214.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_6591"/>
    <dbReference type="EnsemblBacteria" id="ADC45192">
        <property type="protein sequence ID" value="ADC45192"/>
        <property type="gene ID" value="Rmet_6591"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADC45214">
        <property type="protein sequence ID" value="ADC45214"/>
        <property type="gene ID" value="Rmet_6615"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6591"/>
    <dbReference type="KEGG" id="rme:Rmet_6615"/>
    <dbReference type="HOGENOM" id="CLU_2828122_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADC45192.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADC45214.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="66" mass="7188" checksum="F3C59121FB6B862D" modified="2010-03-23" version="1">MNALHNLVSPLQRNDGNVSEAATANRQSRALRFFNNQTTDKCGRLMANATVTSVAVMLQKLSVLAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-03-23" modified="2020-10-07" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>D3DY42</accession>
    <name>D3DY42_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_6613</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ADC45212.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_6613"/>
    <dbReference type="EnsemblBacteria" id="ADC45212">
        <property type="protein sequence ID" value="ADC45212"/>
        <property type="gene ID" value="Rmet_6613"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6613"/>
    <dbReference type="HOGENOM" id="CLU_2275138_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="36"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADC45212.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="102" mass="10513" checksum="B07C85AF09C3938E" modified="2010-03-23" version="1">MLHGHRRRGSRVPVGQRSGSRDDAHLRSRPLGRRDARLAITPAVVIAVATAFAIALSDAATVHDAAILVVTGEIAGNAIATITTTTAGAGLNLSRPHAVHIA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1994-10-01" modified="2022-05-25" version="120" xmlns="http://uniprot.org/uniprot">
    <accession>P37248</accession>
    <accession>Q5NV33</accession>
    <name>TRA8_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName>Transposase for insertion sequence element IS1086</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">IS1086</name>
        <name type="ordered locus">Rmet_2842</name>
        <name type="ordered locus">Rmet_4004</name>
        <name type="ordered locus">Rmet_6282</name>
        <name type="ORF">RMe0033</name>
    </gene>
    <organism>
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <geneLocation type="plasmid">
        <name>pMOL28</name>
    </geneLocation>
    <reference key="1">
        <citation type="journal article" date="1992" name="J. Bacteriol." volume="174" first="8133" last="8138">
            <title>Cloning and sequencing of IS1086, an Alcaligenes eutrophus insertion element related to IS30 and IS4351.</title>
            <authorList>
                <person name="Dong Q."/>
                <person name="Sadouk A."/>
                <person name="van der Lelie D."/>
                <person name="Taghavi S."/>
                <person name="Ferhat A."/>
                <person name="Nuyten J.M."/>
                <person name="Borremans B."/>
                <person name="Mergeay M."/>
                <person name="Toussaint A."/>
            </authorList>
            <dbReference type="PubMed" id="1334071"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC DNA]</scope>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid>megaplasmid CH34</plasmid>
            <plasmid>pMOL28</plasmid>
        </source>
    </reference>
    <reference key="3">
        <citation type="submission" date="2004-10" db="EMBL/GenBank/DDBJ databases">
            <title>Sequence and features of the Ralstonia metallidurans CH34 heavy metal plasmids pMOL28 and pMOL30.</title>
            <authorList>
                <person name="van der Lelie D."/>
                <person name="Monchy S."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="Benotmane M."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Mergeay M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid>pMOL28</plasmid>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Required for the transposition of the insertion element.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the transposase IS30 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">It is uncertain whether Met-1 or Met-15 is the initiator.</text>
    </comment>
    <dbReference type="EMBL" id="X58441">
        <property type="protein sequence ID" value="CAA41347.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09715.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10872.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="X90708">
        <property type="protein sequence ID" value="CAI30179.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP000355">
        <property type="protein sequence ID" value="ABF13141.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011239917.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_161657.1">
        <property type="nucleotide sequence ID" value="NC_006525.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="P37248"/>
    <dbReference type="EnsemblBacteria" id="ABF09715">
        <property type="protein sequence ID" value="ABF09715"/>
        <property type="gene ID" value="Rmet_2842"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF10872">
        <property type="protein sequence ID" value="ABF10872"/>
        <property type="gene ID" value="Rmet_4004"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF13141">
        <property type="protein sequence ID" value="ABF13141"/>
        <property type="gene ID" value="Rmet_6282"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2842"/>
    <dbReference type="KEGG" id="rme:Rmet_4004"/>
    <dbReference type="KEGG" id="rme:Rmet_6282"/>
    <dbReference type="eggNOG" id="COG2826">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035706_0_0_4"/>
    <dbReference type="OMA" id="RSVIWRE"/>
    <dbReference type="OrthoDB" id="1780129at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid pMOL28"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004803">
        <property type="term" value="F:transposase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006313">
        <property type="term" value="P:transposition, DNA-mediated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001584">
        <property type="entry name" value="Integrase_cat-core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025246">
        <property type="entry name" value="IS30-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001598">
        <property type="entry name" value="Transposase_IS30_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13936">
        <property type="entry name" value="HTH_38"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00665">
        <property type="entry name" value="rve"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="SSF53098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50994">
        <property type="entry name" value="INTEGRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01043">
        <property type="entry name" value="TRANSPOSASE_IS30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0233">DNA recombination</keyword>
    <keyword id="KW-0238">DNA-binding</keyword>
    <keyword id="KW-0614">Plasmid</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0814">Transposable element</keyword>
    <keyword id="KW-0815">Transposition</keyword>
    <feature type="chain" id="PRO_0000211894" description="Transposase for insertion sequence element IS1086">
        <location>
            <begin position="1"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="domain" description="Integrase catalytic" evidence="1">
        <location>
            <begin position="176"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="sequence conflict" description="In Ref. 1; CAA41347." evidence="2" ref="1">
        <original>A</original>
        <variation>G</variation>
        <location>
            <position position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00457"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="339" mass="38101" checksum="0CC50CC79AF79DE9" modified="2006-07-11" version="2">MTRTKYQQLQPEERMRIEIWKAEDVSLRAMARRLGRAPSTLMRELRRNATARGGYGAMSAQACRTQRLKASRPVAKLAPDGVLWGVVRHFLDQKWSPQEISATLKRAFPDQPDLNVSHETIYNAIYAYPRGELRRQLIACLRQARTKRLPRSRGTDRRGQIPDMVSIHVRPPEVNDRLMPGHWEGDLIKGAGNQSAVGVLVERMSRAVLLVKMPDATAASALAGFTGKLQSLVAPLRQTLTYDQGREMARHAELSAATGVRVYFCDPHSPWQRGTCENTNGLLRQYLPKGTDLSVYSQEELDAIADSLNGRPRKTLNWHSPLQVLAQVLANPTDRLPVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>Q1L9S6</accession>
    <name>Q1L9S6_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">ParB: chromosome partitioning protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">parB2</name>
        <name evidence="4" type="ordered locus">Rmet_6241</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4 5">
        <name>pMOL28</name>
    </geneLocation>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="5">Plasmid pMOL28</plasmid>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ParB family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000355">
        <property type="protein sequence ID" value="ABF13100.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF13100">
        <property type="protein sequence ID" value="ABF13100"/>
        <property type="gene ID" value="Rmet_6241"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6241"/>
    <dbReference type="HOGENOM" id="CLU_035398_0_0_4"/>
    <dbReference type="OMA" id="TGNCTNR"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid pMOL28"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002197">
        <property type="entry name" value="HTH_Fis"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004437">
        <property type="entry name" value="ParB/RepB/Spo0J"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003115">
        <property type="entry name" value="ParB/Sulfiredoxin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036086">
        <property type="entry name" value="ParB/Sulfiredoxin_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022396">
        <property type="entry name" value="PRTRC_ParB"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02954">
        <property type="entry name" value="HTH_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02195">
        <property type="entry name" value="ParBc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00470">
        <property type="entry name" value="ParB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110849">
        <property type="entry name" value="SSF110849"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00180">
        <property type="entry name" value="parB_part"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03734">
        <property type="entry name" value="PRTRC_parB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0614">Plasmid</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ParB" evidence="3">
        <location>
            <begin position="17"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="354"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="357"/>
            <end position="371"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00470"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF13100.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="574" mass="62385" checksum="7636F29795318A4C" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-10-07" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LAD6</accession>
    <name>Q1LAD6_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_6031</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="1 2">
        <name>pMOL30</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid pMOL30</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000354">
        <property type="protein sequence ID" value="ABF12890.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF12890">
        <property type="protein sequence ID" value="ABF12890"/>
        <property type="gene ID" value="Rmet_6031"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6031"/>
    <dbReference type="HOGENOM" id="CLU_1244486_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid pMOL30"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF12890.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="222" mass="23855" checksum="5161E3C593CDB085" modified="2006-05-30" version="1">MDRRIRRMTMEVKHHLLNPDGSYSPIIPDEPIGGTAAQYASFIQAENERDAEARALMMAGKVVLGAIAERMAAPNDRAQHEAEPLGMRIAEDLAVVFAATMTKTGSVSHQTVDQIKTIEAPVQVVNGDAPNTVDEEAKVLATSLPGVESVAGEDGSMLVRFSDGTEATVTRSFLAWTGDPTFELWLPRDERTGMAKMMPKLSRGEVLNPLGQLAAAAAKRAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LAE4</accession>
    <name>Q1LAE4_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_6023</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2 3">
        <name>pMOL30</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid pMOL30</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000354">
        <property type="protein sequence ID" value="ABF12882.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF12882">
        <property type="protein sequence ID" value="ABF12882"/>
        <property type="gene ID" value="Rmet_6023"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6023"/>
    <dbReference type="HOGENOM" id="CLU_836433_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid pMOL30"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="310"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="313"/>
            <end position="332"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF12882.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="332" mass="36410" checksum="1E62E84D29008C40" modified="2006-05-30" version="1">MQDEARMRQDRQDWCSPALVEFPPETPKGIYETTLKVLRGLIGGGASLSDASNADRFPVPEIWTLYWCLVHASHAAVKAAKVAKDENGISRDFLAKLESETRTSPICTRDGGKLQVAYNAIFEQSEPGMKEAAVGADILLIVAGQGLVPNGFARLFWIQAKKARSGQSPFTLNYAQENGDGLQVDALSKVHEPSKGAFGLYMQYSKDLPYVPSVLVGRLPRGKQQPLTAKLSDLGIRLPELLVGHAIVASEKFGAFADTSEILAYLDDVAEKKPLFIVSVTAERAMELSQELAGNQLLSTVTDHYLQRFGLSPEPENDRETPSQDRGMDFDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-10-07" version="51" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LB52</accession>
    <name>Q1LB52_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_5765</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12624.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF12624">
        <property type="protein sequence ID" value="ABF12624"/>
        <property type="gene ID" value="Rmet_5765"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5765"/>
    <dbReference type="HOGENOM" id="CLU_1814230_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="67"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="95"/>
            <end position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF12624.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="142" mass="14773" checksum="3B5C6BE050CAA1B2" modified="2006-05-30" version="1">MHGTAARRAGTTGSNDSRARGRHASPCATVGGHAIHCDPLRPVAARCGHRRWAPFLLPQEMIMKARIAAAAVAAIASLASFATVTSAAGLTPTTTAMPEGHAHAIGPRDPYTDGGKASRFDVYADTANVTDRRDPYTDGAHS</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LB91</accession>
    <name>Q1LB91_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Chloramphenicol acetyltransferase</fullName>
            <ecNumber evidence="1">2.3.1.28</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">cat</name>
        <name evidence="1" type="ordered locus">Rmet_5726</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EC" id="2.3.1.28" evidence="1"/>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12585.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5726"/>
    <dbReference type="EnsemblBacteria" id="ABF12585">
        <property type="protein sequence ID" value="ABF12585"/>
        <property type="gene ID" value="Rmet_5726"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5726"/>
    <dbReference type="eggNOG" id="COG0110">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_051638_5_0_4"/>
    <dbReference type="OMA" id="VVSGCEF"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008811">
        <property type="term" value="F:chloramphenicol O-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001451">
        <property type="entry name" value="Hexapep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011004">
        <property type="entry name" value="Trimer_LpxA-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14602">
        <property type="entry name" value="Hexapep_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51161">
        <property type="entry name" value="SSF51161"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF12585.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="241" mass="26179" checksum="D9B12E100070319D" modified="2006-05-30" version="1">MREWLLSAIYKGRIMPTENEAERQSADIRKRCAELGGRIAPTAQVSSAVSFDEAIDIGDGAVAYAPAFIGGFTYVNVGAVVFPHVRIGKFCSIGRNVQIGAARHPVEFLSSHPFQFSPALFNRVPGYPALQTEKWRFHAPTTVGNDVWLGTNCVIVAGVKIGDGAVVGAGAVVTKDVAPYEIVGGVPARRIRLRFDEDKVSRLLKTRWWDLPLDSLKDIPFGNIDQAIASIEAIRSSKESA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="102" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LBI1</accession>
    <name>Q1LBI1_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Sulfite reductase (NADPH) flavoprotein alpha-component</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Rmet_5636</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="9">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="9">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12495.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011520039.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5636"/>
    <dbReference type="EnsemblBacteria" id="ABF12495">
        <property type="protein sequence ID" value="ABF12495"/>
        <property type="gene ID" value="Rmet_5636"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5636"/>
    <dbReference type="eggNOG" id="COG0369">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001570_17_7_4"/>
    <dbReference type="OMA" id="QLFEMMP"/>
    <dbReference type="OrthoDB" id="707164at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.990.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017927">
        <property type="entry name" value="FAD-bd_FR_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001094">
        <property type="entry name" value="Flavdoxin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008254">
        <property type="entry name" value="Flavodoxin/NO_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001709">
        <property type="entry name" value="Flavoprot_Pyr_Nucl_cyt_Rdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029039">
        <property type="entry name" value="Flavoprotein-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039261">
        <property type="entry name" value="FNR_nucleotide-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023173">
        <property type="entry name" value="NADPH_Cyt_P450_Rdtase_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001433">
        <property type="entry name" value="OxRdtase_FAD/NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017938">
        <property type="entry name" value="Riboflavin_synthase-like_b-brl"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00258">
        <property type="entry name" value="Flavodoxin_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00175">
        <property type="entry name" value="NAD_binding_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00369">
        <property type="entry name" value="FLAVODOXIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00371">
        <property type="entry name" value="FPNCR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52218">
        <property type="entry name" value="SSF52218"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52343">
        <property type="entry name" value="SSF52343"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63380">
        <property type="entry name" value="SSF63380"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51384">
        <property type="entry name" value="FAD_FR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50902">
        <property type="entry name" value="FLAVODOXIN_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0249">Electron transport</keyword>
    <keyword evidence="2" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="3" id="KW-0288">FMN</keyword>
    <keyword evidence="8" id="KW-0614">Plasmid</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004192983" evidence="5">
        <location>
            <begin position="21"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="domain" description="Flavodoxin-like" evidence="6">
        <location>
            <begin position="70"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="domain" description="FAD-binding FR-type" evidence="7">
        <location>
            <begin position="223"/>
            <end position="394"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ABF12495.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="533" mass="57579" checksum="88E997C79BCF8813" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LBJ2</accession>
    <name>Q1LBJ2_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">L-carnitine dehydratase/bile acid-inducible protein F</fullName>
            <ecNumber evidence="1">2.8.3.16</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_5625</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EC" id="2.8.3.16" evidence="1"/>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12484.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011520030.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5625"/>
    <dbReference type="EnsemblBacteria" id="ABF12484">
        <property type="protein sequence ID" value="ABF12484"/>
        <property type="gene ID" value="Rmet_5625"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5625"/>
    <dbReference type="eggNOG" id="COG1804">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033975_2_1_4"/>
    <dbReference type="OMA" id="IRAGIWL"/>
    <dbReference type="OrthoDB" id="969868at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033608">
        <property type="term" value="F:formyl-CoA transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1540.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10540">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003673">
        <property type="entry name" value="CoA-Trfase_fam_III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044855">
        <property type="entry name" value="CoA-Trfase_III_dom3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023606">
        <property type="entry name" value="CoA-Trfase_III_dom_1_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02515">
        <property type="entry name" value="CoA_transf_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89796">
        <property type="entry name" value="SSF89796"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF12484.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="415" mass="44319" checksum="8A537430426BE3B7" modified="2006-05-30" version="1">MSDSPAIAPGALSGLRVLELAQIMAGPTCGMMLADLGADVIKIEKIDGGDDARGYREPTINGVSAPFLMLNRNKRGIALDLKTPEGREVFLRMVRNADVVIENFRKGTLEKLGLGYDTLSRENPGLIYCAISGYGRTGPFADKGGFDLIAQGYTGLMSITGEEGGPPLRTGNSIADINAGILAAFGILAAYQHRQKTGRGQIVETSLIEAGLQQLYWHAAIHFATGESPGPSGSAHVLATPYQAFPTQDGHIIVGGANEKNWARIAQVLGHPEWVEDPRFRRNADRMRHRDTLLPEMAGILMTRPSADWLAAFDAAGVPAGPVHSIGEALNHPQTLARGMVVRQQHAEAGPIRTLGLPIQLSETPAQYHRPSPRLGEHTRTLLAECGYGEAEIEAMLEAGVVTETAASAATVTEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LBP8</accession>
    <name>Q1LBP8_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transcriptional regulator, ArsR family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_5569</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12428.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519978.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5569"/>
    <dbReference type="EnsemblBacteria" id="ABF12428">
        <property type="protein sequence ID" value="ABF12428"/>
        <property type="gene ID" value="Rmet_5569"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5569"/>
    <dbReference type="eggNOG" id="COG0640">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_077964_0_1_4"/>
    <dbReference type="OMA" id="GRNRYYR"/>
    <dbReference type="OrthoDB" id="1948031at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00090">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011991">
        <property type="entry name" value="ArsR-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001845">
        <property type="entry name" value="HTH_ArsR_DNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00778">
        <property type="entry name" value="HTHARSR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00418">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50987">
        <property type="entry name" value="HTH_ARSR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH arsR-type" evidence="1">
        <location>
            <begin position="1"/>
            <end position="94"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF12428.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="235" mass="24934" checksum="D19BFCDBF0829845" modified="2006-05-30" version="1">MEFAPGISQLAGLLADPGRAAMLWALMDGSARPAGELALIAGLSASSTSGHLTRLSEGGLLVVETRGRNRYYRLATPEIGVAIEALATASLASQPPRMRAVPVSRTTPPALKQARTCYDHLAGELAVGLFERMSQAGWIAVDGTRVDLTGDGTQAMGQLGIDVDAARRKRRQFACTCPDWSERKPHLGGALGAALLQAMLAHGWIEPTSTSRALRVTPRGQNEIGKVAAGENRWR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LBW3</accession>
    <name>Q1LBW3_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Outer membrane protein (Porin)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Rmet_5504</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="7">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="7">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12363.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5504"/>
    <dbReference type="EnsemblBacteria" id="ABF12363">
        <property type="protein sequence ID" value="ABF12363"/>
        <property type="gene ID" value="Rmet_5504"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5504"/>
    <dbReference type="eggNOG" id="COG3203">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038238_2_0_4"/>
    <dbReference type="OMA" id="KNVALAF"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046930">
        <property type="term" value="C:pore complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015288">
        <property type="term" value="F:porin activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006811">
        <property type="term" value="P:ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00342">
        <property type="entry name" value="gram_neg_porins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.160.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033900">
        <property type="entry name" value="Gram_neg_porin_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023614">
        <property type="entry name" value="Porin_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13609">
        <property type="entry name" value="Porin_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0406">Ion transport</keyword>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-0626">Porin</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1134">Transmembrane beta strand</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193300" evidence="4">
        <location>
            <begin position="31"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="domain" description="Porin_4" evidence="5">
        <location>
            <begin position="15"/>
            <end position="342"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF13609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABF12363.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="379" mass="40739" checksum="7FB4106C41038769" modified="2006-05-30" version="1">MQKIGGDKVKRALLASTALGLLLCTGYAHSSVTLYGVVDSNIEYVNKLTNASGASGSVVRNSGGGFASNRWGVRGVEDLGGGLKGIFTVEGGFNVDTGTQFNANKLYDRQTFVGVSSDEYGQLTFGRQYSTIFRALANFSPSAYAPMYEPLSFLLGMNLRNDNSVNYVIQRGSIGARLHWTFGNGVFGAGETPGEIRANAGYGASLEYSAGEFGASIAYDQFYPTLTTLGDNGIGNFRKAAIGAGYNLGKFRFSGGYRWGRNEFSNGSVALRDDFLWFGVNYQVAPKVNLMLAYYYDKLHTLRAQYNGAELDRPNPYQVSFMASYDFSKRTNVYLTTAYSKNVALAFGGLATTAASGAYLLGAGKSSQVGAALGIRHIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LC50</accession>
    <name>Q1LC50_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Transcriptional regulator, LysR family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_5417</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the LysR transcriptional regulatory family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12276.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519824.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5417"/>
    <dbReference type="EnsemblBacteria" id="ABF12276">
        <property type="protein sequence ID" value="ABF12276"/>
        <property type="gene ID" value="Rmet_5417"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5417"/>
    <dbReference type="eggNOG" id="COG0583">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039613_6_0_4"/>
    <dbReference type="OMA" id="VSMWSDF"/>
    <dbReference type="OrthoDB" id="990666at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005119">
        <property type="entry name" value="LysR_subst-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000847">
        <property type="entry name" value="Tscrpt_reg_HTH_LysR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00126">
        <property type="entry name" value="HTH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03466">
        <property type="entry name" value="LysR_substrate"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00039">
        <property type="entry name" value="HTHLYSR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50931">
        <property type="entry name" value="HTH_LYSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH lysR-type" evidence="2">
        <location>
            <begin position="1"/>
            <end position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF12276.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="302" mass="33422" checksum="6D085786D4F6BD53" modified="2006-05-30" version="1">MKLHQLRALAAVSETGSMQEASRLLHVTQPALSKAIKELEASVGAALFVRTNKGVQLTPYGQRLVGHARLISENVRRARQDLEDMKGTVVSEITVAVTPVTALLRPAGACITAFRRDFPSAQLRVQEMRPAQMLEHLREGTIDFALTSQSLATAHGLDCAQACHVPTLIAGRKDHPLRKTRSLGALLEAEWLVLDPLSDTGTPFHQLFVINGMTLPSRVVECTSMTLALEMCRQSDLLVLLSEESRRSRYLVETMDFIDVDETMPDRTIALVSRDRHTLTWAAARLHDALLDALRQEFPTAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="106" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LC54</accession>
    <name>Q1LC54_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Aldehyde dehydrogenase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">calB</name>
        <name evidence="8" type="ordered locus">Rmet_5413</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="9">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="9">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 3 6">Belongs to the aldehyde dehydrogenase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12272.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5413"/>
    <dbReference type="EnsemblBacteria" id="ABF12272">
        <property type="protein sequence ID" value="ABF12272"/>
        <property type="gene ID" value="Rmet_5413"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5413"/>
    <dbReference type="eggNOG" id="COG1012">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005391_3_6_4"/>
    <dbReference type="OMA" id="RRITWAA"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016620">
        <property type="term" value="F:oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006081">
        <property type="term" value="P:cellular aldehyde metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.309.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.605.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016161">
        <property type="entry name" value="Ald_DH/histidinol_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016163">
        <property type="entry name" value="Ald_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029510">
        <property type="entry name" value="Ald_DH_CS_GLU"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016162">
        <property type="entry name" value="Ald_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015590">
        <property type="entry name" value="Aldehyde_DH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012394">
        <property type="entry name" value="Aldehyde_DH_NAD(P)"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43570">
        <property type="entry name" value="PTHR43570"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00171">
        <property type="entry name" value="Aldedh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036492">
        <property type="entry name" value="ALDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53720">
        <property type="entry name" value="SSF53720"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00687">
        <property type="entry name" value="ALDEHYDE_DEHYDR_GLU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3 6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-0614">Plasmid</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aldedh" evidence="7">
        <location>
            <begin position="4"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="active site" evidence="4 5">
        <location>
            <position position="208"/>
        </location>
    </feature>
    <feature type="active site" evidence="4">
        <location>
            <position position="242"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009986"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRNR" id="PIRNR036492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR036492-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ABF12272.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="477" mass="52078" checksum="D9DE108D775D8506" modified="2006-05-30" version="1">MERLFQAQRSAFAAAPYPALADRKQGLRRLRDAVRKHADSLAQAADRDFGGRAHAETMMVDVLPSVLHINHLLGGLRRWMRPSRRSVELLFLGNSAKVVYQPKGVVGIVVPWNFPVYLALGPLATALAAGNRCLIKTSEFAPRTSAALRALLAEAFTEDEVAIVEGDADVARQFTGLPFDHLVFTGSPEVGRHVMRAAAEHLTPVTLELGGKSPAIVSSSANLATAARRIAHGKTVNAGQICVAPDYALVPEALAARFATEVLNASAALFPADRDDYASIIHDRAHQRMNDLLDDARAHGAQVMRSAMPEDGRRVPLHVVLGVTPRMRIAREEIFGPILPVFTYRAFEDVVTHIRGGTRPLAMYYFGDDKAESTTLLQRTHAGGVTLNDWGWHVLNHDLPFGGTGTSGMGNYHGAEGFRELSHAKAVFSERKWFPIELFHPPYGTFIQRLTLRMFLGRPTAAPPVQTEASPVTASQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="95" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LCW6</accession>
    <name>Q1LCW6_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Transcriptional regulator, LysR family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_5151</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the LysR transcriptional regulatory family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12010.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519556.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5151"/>
    <dbReference type="EnsemblBacteria" id="ABF12010">
        <property type="protein sequence ID" value="ABF12010"/>
        <property type="gene ID" value="Rmet_5151"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5151"/>
    <dbReference type="eggNOG" id="COG0583">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039613_6_4_4"/>
    <dbReference type="OMA" id="MESMEDH"/>
    <dbReference type="OrthoDB" id="1439189at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005119">
        <property type="entry name" value="LysR_subst-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000847">
        <property type="entry name" value="Tscrpt_reg_HTH_LysR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00126">
        <property type="entry name" value="HTH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03466">
        <property type="entry name" value="LysR_substrate"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00039">
        <property type="entry name" value="HTHLYSR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50931">
        <property type="entry name" value="HTH_LYSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH lysR-type" evidence="2">
        <location>
            <begin position="1"/>
            <end position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF12010.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="302" mass="32726" checksum="DCBBC1DA1F93EFF2" modified="2006-05-30" version="1">MDLRRIRHFVVLAETLNFRRAAERLHIAQPALTVSIQKLEAELDTTLFLRDARGVALTASGRVALLEAKRILFHAGQFSERVHAAASGLGGTLRVGFVGSTTYQVLPRLVSRFREEYPGIELVLKEATSTSILRSLEEEVLDVGLIRTPLMSRGTATLISLQEDRFVAALPRRNPLAAKQPLTLADLAQERFVMYAQTEASGLHAAAMAACQTAGFLPRITQEATQIQTVLALVESGLGVALVPSIMRRFGSDKLVYSTLANYPNTTSIGIALAFSPHADGPAIRHFREVAIAEFAGDAPAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="97" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LD57</accession>
    <name>Q1LD57_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Transcriptional regulator, TetR family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_5057</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11919.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5057"/>
    <dbReference type="EnsemblBacteria" id="ABF11919">
        <property type="protein sequence ID" value="ABF11919"/>
        <property type="gene ID" value="Rmet_5057"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5057"/>
    <dbReference type="eggNOG" id="COG1309">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_105089_0_0_4"/>
    <dbReference type="OMA" id="HRMLIYY"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001647">
        <property type="entry name" value="HTH_TetR"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00440">
        <property type="entry name" value="TetR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH tetR-type" evidence="2">
        <location>
            <begin position="31"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00440"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF11919.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="214" mass="23682" checksum="EB071747E1D0DE41" modified="2006-05-30" version="1">MPGPRQPRAQRMPKAKTTAADRSSSEQAVQLLDAATEHVLAHGLGDLSLRNVADALGTSHRMLIYYFGSADGFWQALLFRVRHTEQQARMRAFSGGMDTGAAMEAAWDRYSAPGYLPVMQLLFEIYGRAIRDRDRFSGFLEDVVGSWTSMLAERLQQSMNISEEEARLYARVEVATMRGLLLDLITTGDRKGTTAALKYFASKMATPGKGRKGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LDD4</accession>
    <name>Q1LDD4_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Secreted protein (Tn6048)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">mmfD</name>
        <name evidence="2" type="ordered locus">Rmet_3002</name>
        <name evidence="3" type="ordered locus">Rmet_4037</name>
        <name evidence="4" type="ordered locus">Rmet_4980</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid</name>
    </geneLocation>
    <geneLocation type="plasmid" evidence="5">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="submission" date="2006-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of the chromosome of Ralstonia metallidurans CH34.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Copeland A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Barry K."/>
                <person name="Detter J.C."/>
                <person name="Glavina del Rio T."/>
                <person name="Hammon N."/>
                <person name="Israni S."/>
                <person name="Dalin E."/>
                <person name="Tice H."/>
                <person name="Martinez M."/>
                <person name="Goltsman E."/>
                <person name="Pitluck S."/>
                <person name="Schmutz J."/>
                <person name="Larimer F."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Kim E."/>
                <person name="Mergeay M."/>
                <person name="Benotmane M.A."/>
                <person name="Vallaeys T."/>
                <person name="Michaux A."/>
                <person name="Monchy S."/>
                <person name="Dunn J."/>
                <person name="McCorkle S."/>
                <person name="Taghavi S."/>
                <person name="van der Lelie D."/>
                <person name="Richardson P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">CH34</strain>
            <plasmid evidence="4">megaplasmid</plasmid>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="5">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2010-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Benotmane R."/>
                <person name="Lapidus A."/>
                <person name="McCorkle S."/>
                <person name="Monchy S."/>
                <person name="Taghavi S."/>
                <person name="van der Lelie N."/>
                <person name="Dunn J."/>
                <person name="Leys N."/>
                <person name="Mergeay M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">CH34</strain>
            <plasmid evidence="4">megaplasmid</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09875.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10905.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11842.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_005797953.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3002"/>
    <dbReference type="EnsemblBacteria" id="ABF09875">
        <property type="protein sequence ID" value="ABF09875"/>
        <property type="gene ID" value="Rmet_3002"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF10905">
        <property type="protein sequence ID" value="ABF10905"/>
        <property type="gene ID" value="Rmet_4037"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF11842">
        <property type="protein sequence ID" value="ABF11842"/>
        <property type="gene ID" value="Rmet_4980"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3002"/>
    <dbReference type="KEGG" id="rme:Rmet_4037"/>
    <dbReference type="KEGG" id="rme:Rmet_4980"/>
    <dbReference type="eggNOG" id="COG4705">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_148016_0_0_4"/>
    <dbReference type="OMA" id="ADCKAAM"/>
    <dbReference type="OrthoDB" id="1823800at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0614">Plasmid</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5013530854" evidence="1">
        <location>
            <begin position="24"/>
            <end position="149"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF09875.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF10905.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF11842.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="149" mass="15414" checksum="2C8C25A2ADCABDC0" modified="2006-05-30" version="1">MLIKHSIARPVAAISAAVLVVLAAGCSKSADQNNASSASTTPAQTVSALQVKTASKLGDLSAFRSIATDVAAIVDKGDLPAAKSRIKDLEVAWDSAEAGLKPRAADDWHLLDKAIDKSLATLRADTPSQADCKAAMDALLKTFNTLEGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LDE2</accession>
    <name>Q1LDE2_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Transposase (Tn6048)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">tniA</name>
        <name evidence="3" type="ordered locus">Rmet_4972</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11834.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF11834">
        <property type="protein sequence ID" value="ABF11834"/>
        <property type="gene ID" value="Rmet_4972"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4972"/>
    <dbReference type="eggNOG" id="COG2801">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017991_4_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001584">
        <property type="entry name" value="Integrase_cat-core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015378">
        <property type="entry name" value="Transposase-like_Mu_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09299">
        <property type="entry name" value="Mu-transpos_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00665">
        <property type="entry name" value="rve"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="SSF53098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50994">
        <property type="entry name" value="INTEGRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Integrase catalytic" evidence="2">
        <location>
            <begin position="174"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="510"/>
            <end position="562"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="510"/>
            <end position="527"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF11834.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="562" mass="62941" checksum="7E2B7B24BE3EA9A3" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="96" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LDJ5</accession>
    <name>Q1LDJ5_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 8 10">FAD:protein FMN transferase</fullName>
            <ecNumber evidence="2 8 10">2.7.1.180</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Flavin transferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">nosX</name>
        <name evidence="11" type="ordered locus">Rmet_4919</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="12">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="12">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Flavin transferase that catalyzes the transfer of the FMN moiety of FAD and its covalent binding to the hydroxyl group of a threonine residue in a target flavoprotein.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 8 10">
            <text>FAD + L-threonyl-[protein] = AMP + FMN-L-threonyl-[protein] + H(+)</text>
            <dbReference type="Rhea" id="RHEA:36847"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11061"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
            <dbReference type="ChEBI" id="CHEBI:74257"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="2.7.1.180"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <cofactor evidence="9">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="9">Magnesium. Can also use manganese.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Cell inner membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8 10">Belongs to the ApbE family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.180" evidence="2 8 10"/>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11781.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519341.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4919"/>
    <dbReference type="EnsemblBacteria" id="ABF11781">
        <property type="protein sequence ID" value="ABF11781"/>
        <property type="gene ID" value="Rmet_4919"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4919"/>
    <dbReference type="eggNOG" id="COG1477">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_044403_1_2_4"/>
    <dbReference type="OMA" id="SQVMDIN"/>
    <dbReference type="OrthoDB" id="2021063at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017013">
        <property type="term" value="P:protein flavinylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.520.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024932">
        <property type="entry name" value="ApbE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003374">
        <property type="entry name" value="ApbE-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30040">
        <property type="entry name" value="PTHR30040"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02424">
        <property type="entry name" value="ApbE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006268">
        <property type="entry name" value="ApbE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143631">
        <property type="entry name" value="SSF143631"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="10" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8 10" id="KW-0274">FAD</keyword>
    <keyword evidence="4 8 10" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="10 11" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="7 8 9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 8 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0614">Plasmid</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8 10" id="KW-0808">Transferase</keyword>
    <feature type="binding site" evidence="9">
        <location>
            <position position="182"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001219"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011955"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00016337"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRNR" id="PIRNR006268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR006268-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU363002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ABF11781.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="340" mass="36099" checksum="295753F1C89B65E9" modified="2006-05-30" version="1">MHALSPSGLARRRYLTAAPLLALGMFVLPALADSSVRHASRRLLGTRVDIVTDGVPADAAAQAIDAAFAEMGRLERIMSRYRPDSQVSALARAAGKHPVPIAPELMSALRLASEVSARSQGAFDITVGAFEGWNFDPGHEQVPGQAELTQERPLVNYRDVVLDSTRGSAYLRRPGMKLDLGGIAKLPILQAGMDVLRQQGISNAMLNGGGDVLVSGRLQGRDWRIGLRDPLAPERLLGVVAITDGVVASSGDYERCFARDGQRYHHILDPRTGLPTRGPHGVTLVARKTSDVNGLGAAIMVAGTAAGRSLLGPMANVDALIVGPGSHPWMSEGMATRMRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="102" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LDL1</accession>
    <name>Q1LDL1_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative transcriptional regulator, TetR family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_4903</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11765.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519325.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4903"/>
    <dbReference type="EnsemblBacteria" id="ABF11765">
        <property type="protein sequence ID" value="ABF11765"/>
        <property type="gene ID" value="Rmet_4903"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4903"/>
    <dbReference type="eggNOG" id="COG1309">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069356_28_2_4"/>
    <dbReference type="OMA" id="RTCDLGE"/>
    <dbReference type="OrthoDB" id="2047542at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001647">
        <property type="entry name" value="HTH_TetR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036271">
        <property type="entry name" value="Tet_transcr_reg_TetR-rel_C_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00440">
        <property type="entry name" value="TetR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00455">
        <property type="entry name" value="HTHTETR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48498">
        <property type="entry name" value="SSF48498"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50977">
        <property type="entry name" value="HTH_TETR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH tetR-type" evidence="2">
        <location>
            <begin position="10"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="H-T-H motif" evidence="1">
        <location>
            <begin position="33"/>
            <end position="52"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF11765.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="200" mass="21086" checksum="6DE93884B2574837" modified="2006-05-30" version="1">MARASRAVAEQHKAAIEAASARLFREHGLHGVSVAQVMADAGLTHGGFYGHFPSRDALVARACALAFEESATRWRQRIDQAQGDRKAARRNLVVQYLSTTHRDAAGRGCAASALAGDVAREASDKPVHDAYLHGLKSLIDIWQSTAPDGDDAAGDSALVDLATLVGAITIARATRGDALSDDILAAARDRLLASHKPTDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="63" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LDQ1</accession>
    <name>Q1LDQ1_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_4863</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11725.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519285.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4863"/>
    <dbReference type="EnsemblBacteria" id="ABF11725">
        <property type="protein sequence ID" value="ABF11725"/>
        <property type="gene ID" value="Rmet_4863"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4863"/>
    <dbReference type="eggNOG" id="COG3305">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_113251_2_0_4"/>
    <dbReference type="OMA" id="VEAYGLW"/>
    <dbReference type="OrthoDB" id="1920116at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021125">
        <property type="entry name" value="DUF2127"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09900">
        <property type="entry name" value="DUF2127"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="94"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="126"/>
            <end position="144"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF11725.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="154" mass="16513" checksum="C32C155723380FBA" modified="2006-05-30" version="1">MAHSGSPLGLKGIALFEAAKGALVILAGVGLMALLHHDAQEIAETIIRRIHINPASHYPTIFLSLLANPGDGRLWAIGGSAFVYALMRFAEAYGLWRGLAWGNWIGVWSGGIYIPVELYEAIRHPTWLHGGLAIANALVVVYLAQGLARHKVSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LDT9</accession>
    <name>Q1LDT9_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Putative threonine dehydratase</fullName>
            <ecNumber evidence="4">4.3.1.19</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">tdcB</name>
        <name evidence="4" type="ordered locus">Rmet_4825</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="5">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="5">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <dbReference type="EC" id="4.3.1.19" evidence="4"/>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11687.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519250.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4825"/>
    <dbReference type="EnsemblBacteria" id="ABF11687">
        <property type="protein sequence ID" value="ABF11687"/>
        <property type="gene ID" value="Rmet_4825"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4825"/>
    <dbReference type="eggNOG" id="COG1171">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021152_4_2_4"/>
    <dbReference type="OMA" id="QTQHLGQ"/>
    <dbReference type="OrthoDB" id="1876944at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004794">
        <property type="term" value="F:L-threonine ammonia-lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1100">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001926">
        <property type="entry name" value="PLP-dep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036052">
        <property type="entry name" value="Trypto_synt_PLP_dependent"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00291">
        <property type="entry name" value="PALP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53686">
        <property type="entry name" value="SSF53686"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0456">Lyase</keyword>
    <keyword evidence="4" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PALP" evidence="3">
        <location>
            <begin position="20"/>
            <end position="308"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF11687.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="322" mass="34539" checksum="AA8E1369D86BC47A" modified="2006-05-30" version="1">MTTLQLPTYADVEAAAKRIEGFANRTPVNTSRTLNEMLGAEVFFKCENFQRMGAFKFRGAFNALAKFTAEQRRGGVVAFSSGNHAQGIALSAKLLNMPATIVMPHDAPALKVAATRGYGATVVSYDRYKEDREAIGRDLAEKHGMTLIPPYDHPDVLTGQGTAAKELFEEVGALDAIFTPLGGGGLLSGTALTTRALAPQCQIYGVEPEAGNDGQQSFRSGSIVHIDTPQTIADGAQTQHLGQYTFSIIKRDVTDILTATDAQLVEAMRFYAERMKMIVEPTGCLGLAAVMQMKDALKGKRVGVVISGGNVDLERFCSLLSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="59" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LE35</accession>
    <name>Q1LE35_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_4729</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11591.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519165.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF11591">
        <property type="protein sequence ID" value="ABF11591"/>
        <property type="gene ID" value="Rmet_4729"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4729"/>
    <dbReference type="HOGENOM" id="CLU_1414152_0_0_4"/>
    <dbReference type="OMA" id="LQVEAMT"/>
    <dbReference type="OrthoDB" id="1531555at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF11591.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="216" mass="23191" checksum="6902D493F947F7A2" modified="2006-05-30" version="1">MSERLIMLDVPGVLYSDRSATRLGGTPNTGTLRDVRYFDPIALGYLRRLFGIAGARVVVSDAWRRGTPAAIFQQLDLQVEGMTPPVAGGHGAEIDAWFAQHPAPGAWVIISTAAPESLTDEQRAHLVQVNPAEGLTVDIFRKALDILGVACPSTITPDSRVDPDLKAKLRHLQQLAREAPERFMPAPAAAEARERAMAIAVASLTGKQLESGAIHA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="62" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LE56</accession>
    <name>Q1LE56_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_4708</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11570.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519141.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4708"/>
    <dbReference type="EnsemblBacteria" id="ABF11570">
        <property type="protein sequence ID" value="ABF11570"/>
        <property type="gene ID" value="Rmet_4708"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4708"/>
    <dbReference type="eggNOG" id="COG3167">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_115745_0_0_4"/>
    <dbReference type="OMA" id="SYPQIRG"/>
    <dbReference type="OrthoDB" id="1686143at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="21"/>
            <end position="42"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF11570.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="188" mass="20634" checksum="35D8D6F696E0B702" modified="2006-05-30" version="1">MTAAQVRDRLLTIRFALWRTLGTPGLLGIAVLAAAIVVWSMVPDLMADTKALHAQTWQARKVAGIRAAEAAQAKADISAPDKFPELFSTFSGSGEDLTVIFAQARESHLTLGSAQYQFSAEPGARFVRYQVTLPVKDQYGAIRRFIASVLNSVPNAALQEIHVERPAVDGNQIEARIRFALIYRTERP</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="69" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LET0</accession>
    <name>Q1LET0_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Microcystinase C</fullName>
            <shortName evidence="1">MlrC</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Rmet_4481</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="5">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="5">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in peptidolytic degradation of cyclic heptapeptide hepatotoxin microcystin (MC).</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="1">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase M81 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11346.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518944.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4481"/>
    <dbReference type="EnsemblBacteria" id="ABF11346">
        <property type="protein sequence ID" value="ABF11346"/>
        <property type="gene ID" value="Rmet_4481"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4481"/>
    <dbReference type="eggNOG" id="COG5476">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028172_1_0_4"/>
    <dbReference type="OMA" id="FKEFPHT"/>
    <dbReference type="OrthoDB" id="421877at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008237">
        <property type="term" value="F:metallopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009197">
        <property type="entry name" value="MlrC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010799">
        <property type="entry name" value="MlrC_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015995">
        <property type="entry name" value="MlrC_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07364">
        <property type="entry name" value="DUF1485"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07171">
        <property type="entry name" value="MlrC_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF012702">
        <property type="entry name" value="UCP012702"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="4" id="KW-0614">Plasmid</keyword>
    <keyword evidence="1" id="KW-0645">Protease</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF1485" evidence="3">
        <location>
            <begin position="2"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="domain" description="MlrC_C" evidence="2">
        <location>
            <begin position="297"/>
            <end position="478"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRNR" id="PIRNR012702"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF07364"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF11346.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="497" mass="52738" checksum="1C41B93015DC20CD" modified="2006-05-30" version="1">MKILIARLNHETNTYSPVATPLESFSPRYGAQGYRAAKGTRTAAGAFIDLAEAVGAEIVVPVIAGANPSGRVAADAYTHLTDTIVAAAAGCDAVMLDLHGAMVAENSDDGEGELLQRLRQVLPDAPIAVALDLHGNITQPLIDHADIAVSFKTYPHIDMYETGEHAGRLLLEMIAGRVRPVMAWRRPPLVTHTLRSRTDEGAMQRAVALAKEAEKGGMLAVSILAGFGLADIPAPCISVIVVGDGDRRKADEVAERIAAQIWEEREGFTYRPDPLPTSIAMAAAAANATAEHRPVLLLDHGDNCMSGGTCDDMAVLHEALAQGLDGIAVGPVCDPEAVAQLIAAGVGASVTLNVGDKVAAPQLSVYPESKPLSGKVTAISDGEYVISGPTYTGQRIRMGRTVLLDTGAAKVIVTETPQEHWDLGIFTHIGVDPHAARFLILKSRMYCRPVFVPIAKSVIECDSEGVTSSRYEMFPFRNVSRPVYPLDKDTAWSASAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-10-07" version="67" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LF74</accession>
    <name>Q1LF74_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Extra-cytoplasmic solute receptor, conserved protein UPF0065</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">bug</name>
        <name evidence="4" type="ordered locus">Rmet_4337</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="5">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="5">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Periplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the UPF0065 (bug) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11202.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4337"/>
    <dbReference type="EnsemblBacteria" id="ABF11202">
        <property type="protein sequence ID" value="ABF11202"/>
        <property type="gene ID" value="Rmet_4337"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4337"/>
    <dbReference type="eggNOG" id="COG3181">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045683_0_0_4"/>
    <dbReference type="OrthoDB" id="447734at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042597">
        <property type="term" value="C:periplasmic space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005064">
        <property type="entry name" value="BUG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042100">
        <property type="entry name" value="Bug_dom1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42928">
        <property type="entry name" value="PTHR42928"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03401">
        <property type="entry name" value="TctC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017082">
        <property type="entry name" value="YflP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-0675">Receptor</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193405" evidence="3">
        <location>
            <begin position="34"/>
            <end position="335"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF11202.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="335" mass="36714" checksum="7139431904FF255A" modified="2006-05-30" version="1">MKHSHQQRRKIGFNGVIFGMLVCLATWLPEANAAPTDAYPSKPIRVIVPYPAGGIADKVARDAADELGKRLSQTIVVENRPGAAGNIGFEWVARQPADGYTLLLAPASNLTVQPALFKHLSYDLDRDFTPVSLLVLTPQVLVVNPSFPVNSVRELIEYSRAYPNKVNFGASIGSYAHLAGEKLKTETGANFTSIPYQGSVPALNDLMGGQIQFMFTEMATALPYIQGGKLKPLAVAYKNRVSWLPNVPTLKELGLQNFEVTSWYAIVARSGTPPQIVQQLSRELNNIVQAPGFKKRYTDIGATTVGGRPEELGRFARQEAVQWIEEVKRVGIEPN</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LFL4</accession>
    <name>Q1LFL4_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Methyl-accepting chemotaxis sensory transducer</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">cheD</name>
        <name evidence="10" type="ordered locus">Rmet_4195</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="11">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="11">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the methyl-accepting chemotaxis (MCP) protein family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11062.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518685.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4195"/>
    <dbReference type="EnsemblBacteria" id="ABF11062">
        <property type="protein sequence ID" value="ABF11062"/>
        <property type="gene ID" value="Rmet_4195"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4195"/>
    <dbReference type="eggNOG" id="COG0840">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_107_16_4"/>
    <dbReference type="OMA" id="AAMEQFT"/>
    <dbReference type="OrthoDB" id="429006at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004888">
        <property type="term" value="F:transmembrane signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006935">
        <property type="term" value="P:chemotaxis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035440">
        <property type="entry name" value="4HB_MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004090">
        <property type="entry name" value="Chemotax_Me-accpt_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003660">
        <property type="entry name" value="HAMP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004089">
        <property type="entry name" value="MCPsignal_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003122">
        <property type="entry name" value="Tar_rcpt_lig-bd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00672">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00015">
        <property type="entry name" value="MCPsignal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02203">
        <property type="entry name" value="TarH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00260">
        <property type="entry name" value="CHEMTRNSDUCR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00304">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00283">
        <property type="entry name" value="MA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47170">
        <property type="entry name" value="SSF47170"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50111">
        <property type="entry name" value="CHEMOTAXIS_TRANSDUC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50885">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0145">Chemotaxis</keyword>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0488">Methylation</keyword>
    <keyword evidence="10" id="KW-0614">Plasmid</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0807">Transducer</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="188"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="domain" description="HAMP" evidence="9">
        <location>
            <begin position="212"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="domain" description="Methyl-accepting transducer" evidence="8">
        <location>
            <begin position="269"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="469"/>
            <end position="496"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004429"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022500"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00029447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ABF11062.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="515" mass="53568" checksum="E4C355D9C3EE8F58" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-12-02" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LFS0</accession>
    <name>Q1LFS0_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">ABC-type sulfonate/nitrate transporter (Permease component)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Rmet_4139</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="7">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="7">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cell membrane</location>
            <topology evidence="4">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the binding-protein-dependent transport system permease family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11006.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4139"/>
    <dbReference type="EnsemblBacteria" id="ABF11006">
        <property type="protein sequence ID" value="ABF11006"/>
        <property type="gene ID" value="Rmet_4139"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4139"/>
    <dbReference type="eggNOG" id="COG0600">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046113_1_4_4"/>
    <dbReference type="OMA" id="LWQQGWL"/>
    <dbReference type="OrthoDB" id="1531811at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="SSF161098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0614">Plasmid</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2 4" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="46"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="98"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="130"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="157"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="198"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="227"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="259"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="5">
        <location>
            <begin position="88"/>
            <end position="276"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABF11006.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="292" mass="31813" checksum="CBB60DDC96673BC7" modified="2006-05-30" version="1">MQAMSTANPPVRPEYEHLLAPFTAAPLSLPLPWYRQLWQQGWMRKALILVVLATLWEIIARIQDNDLLLPTFLATVRAFGEGVASGELPGKAAVSLTVLLQGYAAGIVLAFALTTLAASTRIGRDLLDTLTAMFNPLPAIALLPLALLWFGLGSKSLIFVLVHSVLWPLALNTYAGFRGVPETLRMAGRNYGLRGLRFVVLILVPAALPAILSGLKIGWAFAWRTLIAAELVFGASSGKGGLGWYIFQNRNELYTDRVFAGLATVILIGLAVEGLVFATLERVTVRRWGQQH</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LG02</accession>
    <name>Q1LG02_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Cytochrome c-type biogenesis protein heme lyase, CcmF subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">ccmF</name>
        <name evidence="11" type="ordered locus">Rmet_4056</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="12">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="12">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell inner membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the CcmF/CycK/Ccl1/NrfE/CcsA family.</text>
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        <property type="protein sequence ID" value="ABF10924.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="InterPro"/>
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    <dbReference type="GO" id="GO:0015232">
        <property type="term" value="F:heme transmembrane transporter activity"/>
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        <property type="project" value="InterPro"/>
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        <property type="term" value="F:lyase activity"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="P:cytochrome complex assembly"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="entry name" value="CcmF_C"/>
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        <property type="entry name" value="Cyt_c_assembly"/>
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        <property type="entry name" value="Cyt_c_biogenesis"/>
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    <dbReference type="InterPro" id="IPR003568">
        <property type="entry name" value="Cyt_c_biogenesis_CcmF"/>
    </dbReference>
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        <property type="entry name" value="PTHR43653"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16327">
        <property type="entry name" value="CcmF_C"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Cytochrom_C_asm"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="CCBIOGENESIS"/>
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        <property type="entry name" value="CCMFBIOGNSIS"/>
    </dbReference>
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        <property type="entry name" value="nrfE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0201">Cytochrome c-type biogenesis</keyword>
    <keyword evidence="11" id="KW-0456">Lyase</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-0614">Plasmid</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="44"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="96"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="121"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="175"/>
            <end position="197"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
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            <end position="229"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
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            <end position="266"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="273"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="312"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="352"/>
            <end position="373"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="393"/>
            <end position="413"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="425"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="449"/>
            <end position="468"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="489"/>
            <end position="509"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="618"/>
            <end position="637"/>
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    </feature>
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        <location>
            <begin position="89"/>
            <end position="295"/>
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    </feature>
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        <location>
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            <end position="640"/>
        </location>
    </feature>
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        <location>
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            <end position="680"/>
        </location>
    </feature>
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        <source>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00009186"/>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022748"/>
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    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
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    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01578"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF16327"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ABF10924.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="680" mass="73410" checksum="0CFE634DB819A1CA" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="106" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LG73</accession>
    <name>Q1LG73_CUPMC</name>
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        </recommendedName>
    </protein>
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        <name type="synonym">Ralstonia metallidurans</name>
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        <lineage>
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        </lineage>
    </organism>
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        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
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                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
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            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
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        </source>
    </reference>
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        <reaction evidence="1">
            <text>ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.</text>
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        <location>
            <begin position="29"/>
            <end position="54"/>
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            <end position="763"/>
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        <location>
            <begin position="918"/>
            <end position="1032"/>
        </location>
    </feature>
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        <location>
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        <location>
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        <location>
            <position position="967"/>
        </location>
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            <position position="1094"/>
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        <source>
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        <source>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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    </evidence>
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            <dbReference type="PROSITE" id="PS50894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ABF10853.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="1156" mass="124702" checksum="CC446A2A59342750" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="72" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LGE1</accession>
    <name>Q1LGE1_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Extra-cytoplasmic solute receptor protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">bug</name>
        <name evidence="4" type="ordered locus">Rmet_3917</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="5">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="5">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Periplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the UPF0065 (bug) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10785.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518432.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3917"/>
    <dbReference type="EnsemblBacteria" id="ABF10785">
        <property type="protein sequence ID" value="ABF10785"/>
        <property type="gene ID" value="Rmet_3917"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3917"/>
    <dbReference type="eggNOG" id="COG3181">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045683_0_0_4"/>
    <dbReference type="OMA" id="RDFVPIG"/>
    <dbReference type="OrthoDB" id="447734at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042597">
        <property type="term" value="C:periplasmic space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005064">
        <property type="entry name" value="BUG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042100">
        <property type="entry name" value="Bug_dom1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42928">
        <property type="entry name" value="PTHR42928"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03401">
        <property type="entry name" value="TctC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017082">
        <property type="entry name" value="YflP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-0675">Receptor</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193436" evidence="3">
        <location>
            <begin position="29"/>
            <end position="328"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF10785.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="328" mass="34430" checksum="E190E5A7FD98F0FE" modified="2006-05-30" version="1">MRSKLRRALLGMTLVLAGVCMTTGAVNAARVYPDRPIRLVVPFPAGGGTDVIGRMLAARLSNTLKTTVVVDNVVGATGTIGTAQVARAAPDGYTLVLGISATHAIAPSIFPKLPYDPLRDFVPIGRIAIGGNVLVANPSFPAHDLRALIAMAKQPNADLTYGSWGQGSGGHLAGESLNVSAGIHLRHIPYKGVAPMLTDVMGGTLPVAMSDLAGTLPLIRSGKVRALAVSGSERSVALPDVPTFAESGVRFRLDSWFALFAPSRTPAPIVEQLDRAMQEAMQDSGLQAQIASLGMRYAPVSRAQFTNQMREDTRTWAALVKSSGAALE</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-10-07" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LGF5</accession>
    <name>Q1LGF5_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_3903</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10771.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3903"/>
    <dbReference type="EnsemblBacteria" id="ABF10771">
        <property type="protein sequence ID" value="ABF10771"/>
        <property type="gene ID" value="Rmet_3903"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3903"/>
    <dbReference type="HOGENOM" id="CLU_1757323_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF10771.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="148" mass="16491" checksum="18ECBE6AF33CAF2D" modified="2006-05-30" version="1">MDRELTIDIVCPVMALHVIVMLTITGHLVVLFRSDGPLTMLVTHLTPFPHYVHDAVCNSPTQYRYDQYLRATRSVSTQQRLRAPSCNAPIARVRHIASNDCAHSKIMSTRPAGSRSSPPHNALTTIAATYRRVFRSTFSALALSNDEC</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LGI6</accession>
    <name>Q1LGI6_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Rmet_3872</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="9">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="9">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the UPF0324 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10740.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518387.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3872"/>
    <dbReference type="EnsemblBacteria" id="ABF10740">
        <property type="protein sequence ID" value="ABF10740"/>
        <property type="gene ID" value="Rmet_3872"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3872"/>
    <dbReference type="eggNOG" id="COG2855">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033541_2_1_4"/>
    <dbReference type="OMA" id="IIVKMIR"/>
    <dbReference type="OrthoDB" id="1856009at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018383">
        <property type="entry name" value="UPF0324_pro"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30106">
        <property type="entry name" value="PTHR30106"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03601">
        <property type="entry name" value="Cons_hypoth698"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0614">Plasmid</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="35"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="64"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="90"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="123"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="151"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="184"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="217"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="246"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="271"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="303"/>
            <end position="321"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ABF10740.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="328" mass="34398" checksum="30BE1803E42C6F60" modified="2006-05-30" version="1">MASRLLSARSTLPGLLLVLALTVPATLLGKAVPLLGGAVFGMLLGLLCRAFLKPSESCKPGIQFASKQLLQYSIVGLGFSLPLHEVVHTGLTSLPISLVTLTVAFTVALVAGHLLRIPEKLKVLIGAGTAICGGSAIAAITPVLQPDDHDMTYALSTIFMFNVVAVVIFPILGKMLGMSDAGFGLWAGTAINDTSSVVAAGYAWSSAAGEYATIVKLTRAMMIVPVTLVIAMLFQRSQPGGSMRRVIPWFIVLFVLASAINEFLPAGVTHVIQQLAPFLVIGALTGVGLSTDFNRLKRAGARPLLLGLIVWVSVALSSLVMQHMTGML</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LGN8</accession>
    <name>Q1LGN8_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">AMP-dependent synthetase and ligase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_3818</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10688.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518334.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3818"/>
    <dbReference type="EnsemblBacteria" id="ABF10688">
        <property type="protein sequence ID" value="ABF10688"/>
        <property type="gene ID" value="Rmet_3818"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3818"/>
    <dbReference type="eggNOG" id="COG0318">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000022_59_0_4"/>
    <dbReference type="OMA" id="NGWHHTG"/>
    <dbReference type="OrthoDB" id="377638at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016874">
        <property type="term" value="F:ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025110">
        <property type="entry name" value="AMP-bd_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045851">
        <property type="entry name" value="AMP-bd_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020845">
        <property type="entry name" value="AMP-binding_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_Synth/Lig"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13193">
        <property type="entry name" value="AMP-binding_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00455">
        <property type="entry name" value="AMP_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0436">Ligase</keyword>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AMP-binding" evidence="1">
        <location>
            <begin position="8"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="domain" description="AMP-binding_C" evidence="2">
        <location>
            <begin position="415"/>
            <end position="490"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF10688.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="501" mass="54277" checksum="15FC7A21662F5B0C" modified="2006-05-30" version="1">MNIVALLDSNVRKYPDKPFLRYAGQSITYAEFDALSRGAAIVLADHGVGAGDRVAVLCFNTPGMVAAMFGAWRLGAVAVPVNHKLQTPEVDYILEHAGVKVCVFDGSLASVVSRLQTPVKLLATSTDAAGFDSFDAQLPAAGTYNALPPADDAIAQILYTSGTTGRPKGCVLTHRAVCMAGMTAALALSMQRDERTLMAMPLWHSSPLNNWMLGTLYVGGTVILLREYHPLHFLQTAQDERATLYFGAPVSYLLPLQMKIDVTAFDLGSVRAWIYGGGPIGADTARQLAQLYRSDRFYQVFGMTETGPSGTVLYPEEQVAKAGSIGRVATPGLDMRVVRTGGADAGPGEIGEIWIKADSNMLGYLDNEAATREVMRDGWYRSGDLARLDADGFLFVVDRTKDMIITGGENVYSKEVEDVLGAHPDVQDVAVIGRPHDQWGETVVAVLVPRPGTQPDPEGIKAFAAERLARYKVPREFLIRDTLPRTPTGKLMKHVLRADFT</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LGP7</accession>
    <name>Q1LGP7_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">L-carnitine dehydratase/bile acid-inducible protein F (CoA-transferase family)</fullName>
            <ecNumber evidence="1">2.8.3.16</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_3809</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EC" id="2.8.3.16" evidence="1"/>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10679.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3809"/>
    <dbReference type="EnsemblBacteria" id="ABF10679">
        <property type="protein sequence ID" value="ABF10679"/>
        <property type="gene ID" value="Rmet_3809"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3809"/>
    <dbReference type="eggNOG" id="COG1804">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033975_1_1_4"/>
    <dbReference type="OMA" id="WCARHDL"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033608">
        <property type="term" value="F:formyl-CoA transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1540.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10540">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003673">
        <property type="entry name" value="CoA-Trfase_fam_III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044855">
        <property type="entry name" value="CoA-Trfase_III_dom3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023606">
        <property type="entry name" value="CoA-Trfase_III_dom_1_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02515">
        <property type="entry name" value="CoA_transf_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89796">
        <property type="entry name" value="SSF89796"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF10679.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="396" mass="42670" checksum="D92681879B94BB6A" modified="2006-05-30" version="1">MPPAVLPLEGVTVVALEHAVAAPFASRQLADLGARVVKIERPGVGDFARGYDKTVHGMSSFFVWANRGKESLSLDLKHPDAADILQQLLDEADVLIQNLAPGAADRLGLGFDALHTRFPKLIVCDISGYGDHGPYRDKKAYDLLIQAAAGLISVTGGEDAPSRAGISIADIAAGMYAYSGILSALLLRARTGVGERVEVTMLEALGEWMSYPLNFAHYGGTPPARHGLAHPSIAPYGHYRTGDGGSVIFGLQNDREWQRFCLNVLDDAALANDPRFTTNLERVNHREALDVEIGKHLNRMTRDELIERLDQGGIANAPLNDMHDVWNHPQLAARQRWRDVATPAGSIQALLPPATLTNAPARMGDVPDLGEHTAAILGKLGYTAAQLEALRQQGAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-10-07" version="52" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LGT1</accession>
    <name>Q1LGT1_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_3775</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10645.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3775"/>
    <dbReference type="EnsemblBacteria" id="ABF10645">
        <property type="protein sequence ID" value="ABF10645"/>
        <property type="gene ID" value="Rmet_3775"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3775"/>
    <dbReference type="HOGENOM" id="CLU_2424764_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="21"/>
            <end position="46"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF10645.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="91" mass="9863" checksum="539B5BC71FA8429C" modified="2006-05-30" version="1">MLSNNSGDLANCAAPERKVWREGDTPARRALADTSSRQRSEVVPPTNDGALRRATLVQGVRGRQVLVQTLMSPPIRLNLHIKALASAPLSH</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LGT9</accession>
    <name>Q1LGT9_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">O-6-alkylguanine-DNA:cysteine-protein methyltransferase</fullName>
            <ecNumber evidence="8">2.1.1.63</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">ogt2</name>
        <name evidence="8" type="ordered locus">Rmet_3767</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="9">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="9">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a 4-O-methyl-thymidine in DNA + L-cysteinyl-[protein] = a thymidine in DNA + S-methyl-L-cysteinyl-[protein]</text>
            <dbReference type="Rhea" id="RHEA:53428"/>
            <dbReference type="Rhea" id="RHEA-COMP:10131"/>
            <dbReference type="Rhea" id="RHEA-COMP:10132"/>
            <dbReference type="Rhea" id="RHEA-COMP:13555"/>
            <dbReference type="Rhea" id="RHEA-COMP:13556"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:82612"/>
            <dbReference type="ChEBI" id="CHEBI:137386"/>
            <dbReference type="ChEBI" id="CHEBI:137387"/>
            <dbReference type="EC" id="2.1.1.63"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>a 6-O-methyl-2'-deoxyguanosine in DNA + L-cysteinyl-[protein] = a 2'-deoxyguanosine in DNA + S-methyl-L-cysteinyl-[protein]</text>
            <dbReference type="Rhea" id="RHEA:24000"/>
            <dbReference type="Rhea" id="RHEA-COMP:10131"/>
            <dbReference type="Rhea" id="RHEA-COMP:10132"/>
            <dbReference type="Rhea" id="RHEA-COMP:11367"/>
            <dbReference type="Rhea" id="RHEA-COMP:11368"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:82612"/>
            <dbReference type="ChEBI" id="CHEBI:85445"/>
            <dbReference type="ChEBI" id="CHEBI:85448"/>
            <dbReference type="EC" id="2.1.1.63"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.1.1.63" evidence="8"/>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10637.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3767"/>
    <dbReference type="EnsemblBacteria" id="ABF10637">
        <property type="protein sequence ID" value="ABF10637"/>
        <property type="gene ID" value="Rmet_3767"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3767"/>
    <dbReference type="eggNOG" id="COG0350">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_52_2_4"/>
    <dbReference type="OMA" id="SACAMNA"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003908">
        <property type="term" value="F:methylated-DNA-[protein]-cysteine S-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006304">
        <property type="term" value="P:DNA modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06445">
        <property type="entry name" value="ATase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001497">
        <property type="entry name" value="MethylDNA_cys_MeTrfase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014048">
        <property type="entry name" value="MethylDNA_cys_MeTrfase_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036217">
        <property type="entry name" value="MethylDNA_cys_MeTrfase_DNAb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008332">
        <property type="entry name" value="MethylG_MeTrfase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036631">
        <property type="entry name" value="MGMT_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01035">
        <property type="entry name" value="DNA_binding_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02870">
        <property type="entry name" value="Methyltransf_1N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46767">
        <property type="entry name" value="SSF46767"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53155">
        <property type="entry name" value="SSF53155"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00589">
        <property type="entry name" value="ogt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00374">
        <property type="entry name" value="MGMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0227">DNA damage</keyword>
    <keyword evidence="5" id="KW-0234">DNA repair</keyword>
    <keyword evidence="3 8" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="8" id="KW-0614">Plasmid</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Methyltransf_1N" evidence="7">
        <location>
            <begin position="4"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="domain" description="DNA_binding_1" evidence="6">
        <location>
            <begin position="84"/>
            <end position="164"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001596"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF02870"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ABF10637.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="183" mass="20141" checksum="2EC6E28940A53542" modified="2006-05-30" version="1">MFLSQLDSPLGAMLLVTDARRVVRALDFADHKSRLYRGLREHYGEVELTEIPAPAEIADALARYFHGELEALKVLRTATSGSALQRRVWAALRRIPVGTTTTYGKLAKALGFDDPRAAVEIGAANGANPIAIVVPCHRVIASNGDLKGYAWGLHRKRWLLEHENAMVAKKMQPETQTAMLPGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="54" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LGV6</accession>
    <name>Q1LGV6_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_3748</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10620.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518268.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3748"/>
    <dbReference type="EnsemblBacteria" id="ABF10620">
        <property type="protein sequence ID" value="ABF10620"/>
        <property type="gene ID" value="Rmet_3748"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3748"/>
    <dbReference type="HOGENOM" id="CLU_2059453_0_0_4"/>
    <dbReference type="OrthoDB" id="2223949at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="40"/>
            <end position="67"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF10620.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="119" mass="13085" checksum="1CE133D09570FDD7" modified="2006-05-30" version="1">MRMIMFLLGLFAIVCVLYGISAGVQTIQRGLARLLNNGRNDEAPAVLPAPITSETPESGSAKEPAPSCQGYIGELRELYELHKQGALSNEEFQDWKHHLLSAMRSMPSVRGSGHFRDTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LGX9</accession>
    <name>Q1LGX9_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uracil-xanthine permease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">pbuX</name>
        <name evidence="8" type="ordered locus">Rmet_3725</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="9">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="9">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the nucleobase:cation symporter-2 (NCS2) (TC 2.A.40) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10597.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518245.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3725"/>
    <dbReference type="DNASU" id="4040696"/>
    <dbReference type="EnsemblBacteria" id="ABF10597">
        <property type="protein sequence ID" value="ABF10597"/>
        <property type="gene ID" value="Rmet_3725"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3725"/>
    <dbReference type="eggNOG" id="COG2233">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017959_8_0_4"/>
    <dbReference type="OMA" id="IKPFFPP"/>
    <dbReference type="OrthoDB" id="1419334at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015205">
        <property type="term" value="F:nucleobase transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006043">
        <property type="entry name" value="NCS2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017588">
        <property type="entry name" value="UacT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006042">
        <property type="entry name" value="Xan_ur_permease"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00860">
        <property type="entry name" value="Xan_ur_permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00801">
        <property type="entry name" value="ncs2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03173">
        <property type="entry name" value="pbuX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01116">
        <property type="entry name" value="XANTH_URACIL_PERMASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0614">Plasmid</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="12"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="50"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="79"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="104"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="129"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="163"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="195"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="237"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="316"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="344"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="375"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="405"/>
            <end position="423"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ABF10597.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="445" mass="44917" checksum="2458E03884E030B8" modified="2006-05-30" version="1">METLLYKVEDRPPILTTVLLAAQHLLAALGGIIAVPLVLGGALKLPPDQIVALVNAALLGSGIVTIIQCKGVGPVGIRLPCVMGTSFTFVGAGISVGLEHGVPGILGASLAGSLVMIVGSFFMPTIRKLFPHTVTGVVVTMIGLSLTPVAIDWAAGGAGSANYAAPGSLAIAGIVLLLVIGFVQWGKGIVSASAVVLGIMIGYLICLALGMVDFSQLHDAPVFALPQPASFGMKFPISGIAAMSLAFLVTIVETTGTFMALGSATRTPMPGKTLARGILCDGVGSAFAALLSSPPVSTFAQNVGVVSLTGVASRHVVALTGGMLVLAGLFPVLGALVVTIPQPVLGGAGLMMFAMIVSAGIRMLGHSAPTKRNSLIIAVSIGCGLAVTIRPDLLAKLPAFVKEVFGSGITVGALVAVVLNLCLPGREVDAIDEEVEGETLVQELA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="112" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LH74</accession>
    <name>Q1LH74_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Multidomain oxidoreductase, (2Fe-2S) and FAD/NAD(P)-binding (AntC-like)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Rmet_3630</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="8">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="8">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10502.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518160.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="SMR" id="Q1LH74"/>
    <dbReference type="STRING" id="266264.Rmet_3630"/>
    <dbReference type="EnsemblBacteria" id="ABF10502">
        <property type="protein sequence ID" value="ABF10502"/>
        <property type="gene ID" value="Rmet_3630"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3630"/>
    <dbReference type="eggNOG" id="COG0543">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG0633">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003827_7_0_4"/>
    <dbReference type="OMA" id="FDIYVCG"/>
    <dbReference type="OrthoDB" id="1834577at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00207">
        <property type="entry name" value="fer2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036010">
        <property type="entry name" value="2Fe-2S_ferredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001041">
        <property type="entry name" value="2Fe-2S_ferredoxin-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006058">
        <property type="entry name" value="2Fe2S_fd_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012675">
        <property type="entry name" value="Beta-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008333">
        <property type="entry name" value="Cbr1-like_FAD-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017927">
        <property type="entry name" value="FAD-bd_FR_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001709">
        <property type="entry name" value="Flavoprot_Pyr_Nucl_cyt_Rdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039261">
        <property type="entry name" value="FNR_nucleotide-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001433">
        <property type="entry name" value="OxRdtase_FAD/NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017938">
        <property type="entry name" value="Riboflavin_synthase-like_b-brl"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00970">
        <property type="entry name" value="FAD_binding_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00111">
        <property type="entry name" value="Fer2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00175">
        <property type="entry name" value="NAD_binding_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00371">
        <property type="entry name" value="FPNCR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52343">
        <property type="entry name" value="SSF52343"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54292">
        <property type="entry name" value="SSF54292"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63380">
        <property type="entry name" value="SSF63380"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00197">
        <property type="entry name" value="2FE2S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51085">
        <property type="entry name" value="2FE2S_FER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51384">
        <property type="entry name" value="FAD_FR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0001">2Fe-2S</keyword>
    <keyword evidence="3" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0614">Plasmid</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="2Fe-2S ferredoxin-type" evidence="5">
        <location>
            <begin position="3"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="domain" description="FAD-binding FR-type" evidence="6">
        <location>
            <begin position="98"/>
            <end position="197"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABF10502.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="334" mass="36448" checksum="51E46A208C2B50A5" modified="2006-05-30" version="1">MSYRVEIAETQQVFMVAPGESVLDAALRCGVNLAHECTFGGCGTCRVRVVDGAVTYEEFPMGLTEEEDAAGFALACQARPAGDLVISTARAAEPAIPARRTTATVQAITSLGPDVLHLRLALSDDEAFAFEPGQYMKILLEDGSHRSFSMASAPNGNTVDFHVRQIAGGRFTTGQLPQLRPGDKLDVEVPHGSFSLRKEDYRPLLMVATGTGLAPIKSMLEALMDDPDCPPVWLYWGMRSAADLYLHDEIARWGERLYDFQYVPVLSRADDTWQGRRGYVHDAVAADIGDLSEHAIYLCGSPNMIHDAKQTFMVLGAQAPFIYSDGFTFQHAPG</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="100" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LHD9</accession>
    <name>Q1LHD9_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Peptide deformylase</fullName>
            <shortName evidence="2">PDF</shortName>
            <ecNumber evidence="2">3.5.1.88</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">Polypeptide deformylase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="2" type="primary">def</name>
        <name evidence="3" type="ordered locus">Rmet_3565</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>H2O + N-terminal N-formyl-L-methionyl-[peptide] = formate + N-terminal L-methionyl-[peptide]</text>
            <dbReference type="Rhea" id="RHEA:24420"/>
            <dbReference type="Rhea" id="RHEA-COMP:10639"/>
            <dbReference type="Rhea" id="RHEA-COMP:10640"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15740"/>
            <dbReference type="ChEBI" id="CHEBI:49298"/>
            <dbReference type="ChEBI" id="CHEBI:64731"/>
            <dbReference type="EC" id="3.5.1.88"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
        <text evidence="2">Binds 1 Fe(2+) ion.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the polypeptide deformylase family.</text>
    </comment>
    <dbReference type="EC" id="3.5.1.88" evidence="2"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10437.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517980.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3565"/>
    <dbReference type="EnsemblBacteria" id="ABF10437">
        <property type="protein sequence ID" value="ABF10437"/>
        <property type="gene ID" value="Rmet_3565"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3565"/>
    <dbReference type="eggNOG" id="COG0242">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_061901_2_1_4"/>
    <dbReference type="OMA" id="VCIQHEI"/>
    <dbReference type="OrthoDB" id="1649129at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042586">
        <property type="term" value="F:peptide deformylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00487">
        <property type="entry name" value="Pep_deformylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.45.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00163">
        <property type="entry name" value="Pep_deformylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023635">
        <property type="entry name" value="Peptide_deformylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036821">
        <property type="entry name" value="Peptide_deformylase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10458">
        <property type="entry name" value="PTHR10458"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01327">
        <property type="entry name" value="Pep_deformylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004749">
        <property type="entry name" value="Pep_def"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01576">
        <property type="entry name" value="PDEFORMYLASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56420">
        <property type="entry name" value="SSF56420"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00079">
        <property type="entry name" value="pept_deformyl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0408">Iron</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" evidence="2">
        <location>
            <position position="135"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="92"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="134"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="138"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF10437.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="168" mass="19407" checksum="020E66EDE71CFAD7" modified="2006-05-30" version="1">MAKLDILTYPDPRLHKVAKPVEVVDDRIRQLVKDMAETMYEAPGIGLAATQVDVHERVIVIDISESRDELMVFINPEVTWASENRKVWEEGCLSVPEVYDRVERPDRVKVRALDEKGEAFELDADDLLAVCIQHEIDHLDGKVFVEYLSPLKLQRIKSKLQKRVRSRM</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-01-15" modified="2022-08-03" version="92" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LHJ8</accession>
    <name>MNMG_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Glucose-inhibited division protein A</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">mnmG</name>
        <name evidence="1" type="synonym">gidA</name>
        <name type="ordered locus">Rmet_3506</name>
    </gene>
    <organism>
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">NAD-binding protein involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA-cmnm(5)s(2)U34.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer. Heterotetramer of two MnmE and two MnmG subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the MnmG family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10378.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517930.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q1LHJ8"/>
    <dbReference type="SMR" id="Q1LHJ8"/>
    <dbReference type="STRING" id="266264.Rmet_3506"/>
    <dbReference type="PRIDE" id="Q1LHJ8"/>
    <dbReference type="EnsemblBacteria" id="ABF10378">
        <property type="protein sequence ID" value="ABF10378"/>
        <property type="gene ID" value="Rmet_3506"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3506"/>
    <dbReference type="eggNOG" id="COG0445">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007831_2_2_4"/>
    <dbReference type="OMA" id="FRPGYAI"/>
    <dbReference type="OrthoDB" id="146811at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002098">
        <property type="term" value="P:tRNA wobble uridine modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.570">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00129">
        <property type="entry name" value="MnmG_GidA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004416">
        <property type="entry name" value="MnmG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002218">
        <property type="entry name" value="MnmG-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020595">
        <property type="entry name" value="MnmG-rel_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026904">
        <property type="entry name" value="MnmG_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044920">
        <property type="entry name" value="MnmG_C_subdom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040131">
        <property type="entry name" value="MnmG_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11806">
        <property type="entry name" value="PTHR11806"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11806:SF0">
        <property type="entry name" value="PTHR11806:SF0"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01134">
        <property type="entry name" value="GIDA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13932">
        <property type="entry name" value="GIDA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="SSF51905"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00136">
        <property type="entry name" value="gidA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01280">
        <property type="entry name" value="GIDA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01281">
        <property type="entry name" value="GIDA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0274">FAD</keyword>
    <keyword id="KW-0285">Flavoprotein</keyword>
    <keyword id="KW-0520">NAD</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0819">tRNA processing</keyword>
    <feature type="chain" id="PRO_1000016656" description="tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG">
        <location>
            <begin position="1"/>
            <end position="654"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="13"/>
            <end position="18"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="274"/>
            <end position="288"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00129"/>
        </source>
    </evidence>
    <sequence length="654" mass="71749" checksum="5D91C285B4119178" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-01-15" modified="2022-08-03" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LHL3</accession>
    <name>DCUP_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Uroporphyrinogen decarboxylase</fullName>
            <shortName evidence="1">UPD</shortName>
            <shortName evidence="1">URO-D</shortName>
            <ecNumber evidence="1">4.1.1.37</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">hemE</name>
        <name type="ordered locus">Rmet_3491</name>
    </gene>
    <organism>
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the decarboxylation of four acetate groups of uroporphyrinogen-III to yield coproporphyrinogen-III.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>4 H(+) + uroporphyrinogen III = 4 CO2 + coproporphyrinogen III</text>
            <dbReference type="Rhea" id="RHEA:19865"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57308"/>
            <dbReference type="ChEBI" id="CHEBI:57309"/>
            <dbReference type="EC" id="4.1.1.37"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Porphyrin-containing compound metabolism; protoporphyrin-IX biosynthesis; coproporphyrinogen-III from 5-aminolevulinate: step 4/4.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the uroporphyrinogen decarboxylase family.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.37" evidence="1"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10363.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008650152.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q1LHL3"/>
    <dbReference type="SMR" id="Q1LHL3"/>
    <dbReference type="STRING" id="266264.Rmet_3491"/>
    <dbReference type="EnsemblBacteria" id="ABF10363">
        <property type="protein sequence ID" value="ABF10363"/>
        <property type="gene ID" value="Rmet_3491"/>
    </dbReference>
    <dbReference type="GeneID" id="60822597"/>
    <dbReference type="KEGG" id="rme:Rmet_3491"/>
    <dbReference type="eggNOG" id="COG0407">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_040933_0_0_4"/>
    <dbReference type="OMA" id="LWLMRQA"/>
    <dbReference type="OrthoDB" id="1104410at2"/>
    <dbReference type="UniPathway" id="UPA00251">
        <property type="reaction ID" value="UER00321"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004853">
        <property type="term" value="F:uroporphyrinogen decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006782">
        <property type="term" value="P:protoporphyrinogen IX biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00717">
        <property type="entry name" value="URO-D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.210">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00218">
        <property type="entry name" value="URO_D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038071">
        <property type="entry name" value="UROD/MetE-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006361">
        <property type="entry name" value="Uroporphyrinogen_deCO2ase_HemE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000257">
        <property type="entry name" value="Uroporphyrinogen_deCOase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01208">
        <property type="entry name" value="URO-D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51726">
        <property type="entry name" value="SSF51726"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01464">
        <property type="entry name" value="hemE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00906">
        <property type="entry name" value="UROD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00907">
        <property type="entry name" value="UROD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0210">Decarboxylase</keyword>
    <keyword id="KW-0456">Lyase</keyword>
    <keyword id="KW-0627">Porphyrin biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_1000023956" description="Uroporphyrinogen decarboxylase">
        <location>
            <begin position="1"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="27"/>
            <end position="31"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="77"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="157"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="212"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="333"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="1">
        <location>
            <position position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00218"/>
        </source>
    </evidence>
    <sequence length="367" mass="39923" checksum="9A092DBEE25B6E2B" modified="2006-05-30" version="1">MTALQNDTFLRALRRQPTEYTPLWLMRQAGRYLPEYNATRARAGSFLGLAKNPAYATEVTLQPLDRYPLDAAILFSDILTVPDAMGLGLSFAQGEGPRFAHPLRTEADVAKLAVPDMASLQYVFDAVSEIRKALVQDGRQRVPLIGFSGSPWTLACYMVEGGGSDDFRTVKAMMYARPDLMHRILDINATAVSAYLNAQIEAGAQAVMVFDTWGGALADGMYQAFSLAYMRKVLQGLKREHDGQQIPVIVFTKGGGIWLEEIAGIGPDAIGLDWTVNLAKARRRTEGRVALQGNIDPTVLFASESAIREQVRGVLDSYASAGGSDGHVFNLGHGISQFTPPENVSVLVDEVHNHSRKLRAGQVAAAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="98" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LI34</accession>
    <name>Q1LI34_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Putative short chain dehydrogenase/reductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_3320</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10192.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517771.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3320"/>
    <dbReference type="EnsemblBacteria" id="ABF10192">
        <property type="protein sequence ID" value="ABF10192"/>
        <property type="gene ID" value="Rmet_3320"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3320"/>
    <dbReference type="eggNOG" id="COG1028">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010194_1_0_4"/>
    <dbReference type="OMA" id="NAAHAHR"/>
    <dbReference type="OrthoDB" id="1356861at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00061">
        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF10192.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="262" mass="27406" checksum="CC1962DF1C43DE13" modified="2006-05-30" version="1">MQGLQDKVAIVTGGATLIGAGVARAFAEAGTRVAILDIDAAGGERVAALLGPMAMFVPTDITQDDQVRSAVAQVVSRFGGVDFLVNLACTYLDDGFRSSRADWLAALDVNVVSGVMLAQAVHPHMRTRGGGAIVNFTSISARVAQTGRWLYPVSKAAMAQLTRSMAMDLAPDRIRVNAVSPGWTWCRLMDEVSGGNRARTDAVAAPFHMLGRVGEPDEVAQVVLFLCSDHASFVTGADYAVDGGYAAMGPEQNVPAIGKLAG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2007-06-12" modified="2022-05-25" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LI51</accession>
    <name>RS8_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">30S ribosomal protein S8</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpsH</name>
        <name type="ordered locus">Rmet_3303</name>
    </gene>
    <organism>
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">One of the primary rRNA binding proteins, it binds directly to 16S rRNA central domain where it helps coordinate assembly of the platform of the 30S subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the 30S ribosomal subunit. Contacts proteins S5 and S12.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uS8 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10175.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517767.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q1LI51"/>
    <dbReference type="SMR" id="Q1LI51"/>
    <dbReference type="STRING" id="266264.Rmet_3303"/>
    <dbReference type="EnsemblBacteria" id="ABF10175">
        <property type="protein sequence ID" value="ABF10175"/>
        <property type="gene ID" value="Rmet_3303"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3303"/>
    <dbReference type="eggNOG" id="COG0096">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_098428_0_0_4"/>
    <dbReference type="OMA" id="FDQGYIL"/>
    <dbReference type="OrthoDB" id="1692188at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01302_B">
        <property type="entry name" value="Ribosomal_S8_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000630">
        <property type="entry name" value="Ribosomal_S8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035987">
        <property type="entry name" value="Ribosomal_S8_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11758">
        <property type="entry name" value="PTHR11758"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00410">
        <property type="entry name" value="Ribosomal_S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56047">
        <property type="entry name" value="SSF56047"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00053">
        <property type="entry name" value="RIBOSOMAL_S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <feature type="chain" id="PRO_0000290909" description="30S ribosomal protein S8">
        <location>
            <begin position="1"/>
            <end position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01302"/>
        </source>
    </evidence>
    <sequence length="131" mass="14107" checksum="09E1D10C0AA2C80C" modified="2006-05-30" version="1">MSMSDPIADMLTRIRNAQGVQKASVVMPSSKLKAAIAKVLKDEGYVEDFSVQEEGGKAQLTIGLKYYAGRPVIERIERVSKPGLRVYKGRSDIPHVMNGLGVAIISTPQGLMTDRKARATGVGGEVLCYVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LIA3</accession>
    <name>Q1LIA3_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative DNA-binding transcriptional regulator (BolA-like protein)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_3251</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the BolA/IbaG family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10123.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517725.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3251"/>
    <dbReference type="EnsemblBacteria" id="ABF10123">
        <property type="protein sequence ID" value="ABF10123"/>
        <property type="gene ID" value="Rmet_3251"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3251"/>
    <dbReference type="eggNOG" id="COG5007">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_109462_4_1_4"/>
    <dbReference type="OMA" id="HQLVYGA"/>
    <dbReference type="OrthoDB" id="2009812at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002634">
        <property type="entry name" value="BolA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036065">
        <property type="entry name" value="BolA-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01722">
        <property type="entry name" value="BolA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF003113">
        <property type="entry name" value="BolA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82657">
        <property type="entry name" value="SSF82657"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU003860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF10123.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="81" mass="9119" checksum="B1AB26F699D63FCD" modified="2006-05-30" version="1">MLPTPEQVRDYIAQGLPCEHLEVDGDGQHFFATIVSAEFDGKRLIQRHQRVYAALGERMRAEIHALSMKTLTPAEWQAGGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="111" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LIC4</accession>
    <name>Q1LIC4_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 18">Ubiquinol-cytochrome c reductase iron-sulfur subunit</fullName>
            <ecNumber evidence="5 18">7.1.1.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="primary">petA</name>
        <name evidence="21" type="ordered locus">Rmet_3230</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="22">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Component of the ubiquinol-cytochrome c reductase complex (complex III or cytochrome b-c1 complex), which is a respiratory chain that generates an electrochemical potential coupled to ATP synthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17 18">
            <text>a quinol + 2 Fe(III)-[cytochrome c](out) = a quinone + 2 Fe(II)-[cytochrome c](out) + 2 H(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:11484"/>
            <dbReference type="Rhea" id="RHEA-COMP:10350"/>
            <dbReference type="Rhea" id="RHEA-COMP:14399"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:24646"/>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
            <dbReference type="ChEBI" id="CHEBI:132124"/>
            <dbReference type="EC" id="7.1.1.8"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="18">
            <name>2Fe-2S cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49601"/>
        </cofactor>
        <text evidence="18">Binds 1 [2Fe-2S] cluster per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4 19">The main subunits of complex b-c1 are: cytochrome b, cytochrome c1 and the Rieske protein.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="miscellaneous">
        <text evidence="18">The Rieske protein is a high potential 2Fe-2S protein.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the Rieske iron-sulfur protein family.</text>
    </comment>
    <dbReference type="EC" id="7.1.1.8" evidence="5 18"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10102.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517712.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3230"/>
    <dbReference type="EnsemblBacteria" id="ABF10102">
        <property type="protein sequence ID" value="ABF10102"/>
        <property type="gene ID" value="Rmet_3230"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3230"/>
    <dbReference type="eggNOG" id="COG0723">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_055690_0_2_4"/>
    <dbReference type="OMA" id="KRTWLIA"/>
    <dbReference type="OrthoDB" id="1632945at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008121">
        <property type="term" value="F:ubiquinol-cytochrome-c reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019439">
        <property type="term" value="P:aromatic compound catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.102.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017941">
        <property type="entry name" value="Rieske_2Fe-2S"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036922">
        <property type="entry name" value="Rieske_2Fe-2S_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014349">
        <property type="entry name" value="Rieske_Fe-S_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005805">
        <property type="entry name" value="Rieske_Fe-S_prot_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006311">
        <property type="entry name" value="TAT_signal"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019470">
        <property type="entry name" value="Ubiq_cytC_Rdtase_Fe-S_su_TAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006317">
        <property type="entry name" value="Ubiquinol_cyt_c_Rdtase_Fe-S-su"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10134">
        <property type="entry name" value="PTHR10134"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00355">
        <property type="entry name" value="Rieske"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10399">
        <property type="entry name" value="UCR_Fe-S_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00162">
        <property type="entry name" value="RIESKE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50022">
        <property type="entry name" value="SSF50022"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01416">
        <property type="entry name" value="Rieske_proteo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51296">
        <property type="entry name" value="RIESKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51318">
        <property type="entry name" value="TAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0001">2Fe-2S</keyword>
    <keyword evidence="11" id="KW-0058">Aromatic hydrocarbons catabolism</keyword>
    <keyword evidence="7" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="16" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="18" id="KW-0249">Electron transport</keyword>
    <keyword evidence="13" id="KW-0408">Iron</keyword>
    <keyword evidence="14" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="15 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="21" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12 18" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="18" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="17"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="domain" description="Rieske" evidence="20">
        <location>
            <begin position="90"/>
            <end position="196"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002444"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012951"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00019816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022797"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00029351"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="RuleBase" id="RU004494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="RuleBase" id="RU004497"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS51296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="ABF10102.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="205" mass="21777" checksum="E342F5A09AE734A7" modified="2006-05-30" version="1">MSDQQDVKSVDKGRRNWLIATSVAGGVGGVAVAVPFVSTFAPSEKAKAAGAPVEADIGALKPGEMMTVEWRGKPVWILRRTPEMMASLKKTDAEVADPNSDVPFTMPTPEYCKNESRSRADHKDLLVVVGICSHLGCSPSGPFPAGANPQLGADPGFLCPCHGSTFDLAGRVFKNKPAPQNLDVPPYQFLTDTKLVIGKDEKGEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="107" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LIH1</accession>
    <name>Q1LIH1_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 8 10">Uracil-DNA glycosylase</fullName>
            <shortName evidence="8">UDG</shortName>
            <ecNumber evidence="4 8 10">3.2.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8 12" type="primary">ung</name>
        <name evidence="12" type="ordered locus">Rmet_3183</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2 8 10">Excises uracil residues from the DNA which can arise as a result of misincorporation of dUMP residues by DNA polymerase or due to deamination of cytosine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 8 10">
            <text>Hydrolyzes single-stranded DNA or mismatched double-stranded DNA and polynucleotides, releasing free uracil.</text>
            <dbReference type="EC" id="3.2.2.27"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 8 10">Belongs to the uracil-DNA glycosylase (UDG) superfamily. UNG family.</text>
    </comment>
    <dbReference type="EC" id="3.2.2.27" evidence="4 8 10"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10055.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517664.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3183"/>
    <dbReference type="EnsemblBacteria" id="ABF10055">
        <property type="protein sequence ID" value="ABF10055"/>
        <property type="gene ID" value="Rmet_3183"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3183"/>
    <dbReference type="eggNOG" id="COG0692">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032162_3_0_4"/>
    <dbReference type="OMA" id="PDNGYLM"/>
    <dbReference type="OrthoDB" id="1260295at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004844">
        <property type="term" value="F:uracil DNA N-glycosylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006284">
        <property type="term" value="P:base-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd10027">
        <property type="entry name" value="UDG-F1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.470.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00148">
        <property type="entry name" value="UDG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002043">
        <property type="entry name" value="UDG_fam1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018085">
        <property type="entry name" value="Ura-DNA_Glyclase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005122">
        <property type="entry name" value="Uracil-DNA_glycosylase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036895">
        <property type="entry name" value="Uracil-DNA_glycosylase-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11264">
        <property type="entry name" value="PTHR11264"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03167">
        <property type="entry name" value="UDG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00986">
        <property type="entry name" value="UDG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52141">
        <property type="entry name" value="SSF52141"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00628">
        <property type="entry name" value="ung"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00130">
        <property type="entry name" value="U_DNA_GLYCOSYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 8 10" id="KW-0227">DNA damage</keyword>
    <keyword evidence="7 8 10" id="KW-0234">DNA repair</keyword>
    <keyword evidence="12" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="6 8 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UDG" evidence="11">
        <location>
            <begin position="74"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="8 9">
        <location>
            <position position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001400"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002631"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00148"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU003780"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00986"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ABF10055.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="256" mass="27446" checksum="C87FF65FE284600A" modified="2006-05-30" version="1">MQADLFAPDTPETSSAAPQTVSLQEQANALPAAWRTLLAPCLGKAEWSSLCAFVDEERAAGKPVFPHAVFHALHLTPPDAVKVVILGQDPYHGTGVVDGLEIPQAHGLAFSVPEGVKVPPSLRNIFKEIGAEYGVAPTRASGNLEGWARQGVLLLNTVLTVEQGQAASHAKRGWEAVTDCVIHALAASRPNLVFLLWGSHAQAKRALLEGQTHCVLEAPHPSPLSAHRGFLGCGHFRQANDWLERHGRDGIDWQAT</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-01-15" modified="2022-08-03" version="100" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LIM7</accession>
    <name>MURC_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">UDP-N-acetylmuramate--L-alanine ligase</fullName>
            <ecNumber evidence="1">6.3.2.8</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">UDP-N-acetylmuramoyl-L-alanine synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">murC</name>
        <name type="ordered locus">Rmet_3127</name>
    </gene>
    <organism>
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Cell wall formation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + L-alanine + UDP-N-acetyl-alpha-D-muramate = ADP + H(+) + phosphate + UDP-N-acetyl-alpha-D-muramoyl-L-alanine</text>
            <dbReference type="Rhea" id="RHEA:23372"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57972"/>
            <dbReference type="ChEBI" id="CHEBI:70757"/>
            <dbReference type="ChEBI" id="CHEBI:83898"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.2.8"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cell wall biogenesis; peptidoglycan biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the MurCDEF family.</text>
    </comment>
    <dbReference type="EC" id="6.3.2.8" evidence="1"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09999.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517619.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q1LIM7"/>
    <dbReference type="SMR" id="Q1LIM7"/>
    <dbReference type="STRING" id="266264.Rmet_3127"/>
    <dbReference type="EnsemblBacteria" id="ABF09999">
        <property type="protein sequence ID" value="ABF09999"/>
        <property type="gene ID" value="Rmet_3127"/>
    </dbReference>
    <dbReference type="GeneID" id="60820497"/>
    <dbReference type="KEGG" id="rme:Rmet_3127"/>
    <dbReference type="eggNOG" id="COG0773">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028104_2_2_4"/>
    <dbReference type="OMA" id="DITYQLR"/>
    <dbReference type="OrthoDB" id="307881at2"/>
    <dbReference type="UniPathway" id="UPA00219"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008763">
        <property type="term" value="F:UDP-N-acetylmuramate-L-alanine ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007049">
        <property type="term" value="P:cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009252">
        <property type="term" value="P:peptidoglycan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008360">
        <property type="term" value="P:regulation of cell shape"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1190.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00046">
        <property type="entry name" value="MurC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036565">
        <property type="entry name" value="Mur-like_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004101">
        <property type="entry name" value="Mur_ligase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036615">
        <property type="entry name" value="Mur_ligase_C_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013221">
        <property type="entry name" value="Mur_ligase_cen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000713">
        <property type="entry name" value="Mur_ligase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005758">
        <property type="entry name" value="UDP-N-AcMur_Ala_ligase_MurC"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01225">
        <property type="entry name" value="Mur_ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02875">
        <property type="entry name" value="Mur_ligase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08245">
        <property type="entry name" value="Mur_ligase_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53244">
        <property type="entry name" value="SSF53244"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53623">
        <property type="entry name" value="SSF53623"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01082">
        <property type="entry name" value="murC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0131">Cell cycle</keyword>
    <keyword id="KW-0132">Cell division</keyword>
    <keyword id="KW-0133">Cell shape</keyword>
    <keyword id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0573">Peptidoglycan synthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_1000004391" description="UDP-N-acetylmuramate--L-alanine ligase">
        <location>
            <begin position="1"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="112"/>
            <end position="118"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00046"/>
        </source>
    </evidence>
    <sequence length="471" mass="50016" checksum="DC49203ECCDE61D5" modified="2006-05-30" version="1">MKHIVKNIHFVGIGGAGMSGIAEVLLNLGYKVSGSDVGSNAATRRLASLGARVAHGHDAENVTGANAVVVSTAVTNDNPEVLAARARRIPVVPRAVMLAELMRLKQGVAIAGTHGKTTTTSLVASVLAEGGLDPTFVIGGRLNSAGANARLGTGDFIVAEADESDASFLNLFPVMEVITNIDADHMDTYGHDFARLKQAFVEFTQRLPFYGIAVLCVDDPNVREILPFVSKPVVRYGFAEDAQIRAVNARAVDGQMHFTVLRQLNGHAEPPLDIVLNLPGIHNVQNALAAIAIATELEVPDASIVKALREFHGVGRRFQRYGEVATPDGSGTFTLVDDYGHHPVEMAATLAAARGAFPDRRLVLAFQPHRFTRTRDCFEDFVKVLGTVDALLLAEVYAAGESPIVAADGRALTRALRVANKVEPVFVEQIEDMPQAILNAVRPGDVVVTMGAGSIGAVPGQLVSHQQGGQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-10-07" version="64" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LIS2</accession>
    <name>Q1LIS2_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_3082</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09954.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF09954">
        <property type="protein sequence ID" value="ABF09954"/>
        <property type="gene ID" value="Rmet_3082"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3082"/>
    <dbReference type="eggNOG" id="ENOG50316GW">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_129297_0_0_4"/>
    <dbReference type="OMA" id="RANYMAC"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF09954.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="178" mass="19183" checksum="278015EA50B748CC" modified="2006-05-30" version="1">MASETQIIRPGLWSMHQTIAPFSSDAGIAASQYSEWTPLRRLAVTTLPMVALLAACSSSQPPAESATQAAAQESVQVAPAWQAERVKYMECVQVKVDDGVSSKAVPADVAGGALSACQDQLKVMHDAFQSYLMAGMSSAHGRSSAKQAADRVTTDTREKARVYLTRYVEVERYKAGQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-04-07" version="96" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LJ38</accession>
    <name>Q1LJ38_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7 15">NAD(P) transhydrogenase subunit beta</fullName>
            <ecNumber evidence="6 15">7.1.1.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">Nicotinamide nucleotide transhydrogenase subunit beta</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="primary">pntB</name>
        <name evidence="18" type="ordered locus">Rmet_2965</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="19" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2 15">The transhydrogenation between NADH and NADP is coupled to respiration and ATP hydrolysis and functions as a proton pump across the membrane.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 15">
            <text>H(+)(in) + NAD(+) + NADPH = H(+)(out) + NADH + NADP(+)</text>
            <dbReference type="Rhea" id="RHEA:47992"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="7.1.1.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cell inner membrane</location>
            <topology evidence="4">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5 15">Belongs to the PNT beta subunit family.</text>
    </comment>
    <dbReference type="EC" id="7.1.1.1" evidence="6 15"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09838.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2965"/>
    <dbReference type="DNASU" id="4039793"/>
    <dbReference type="EnsemblBacteria" id="ABF09838">
        <property type="protein sequence ID" value="ABF09838"/>
        <property type="gene ID" value="Rmet_2965"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2965"/>
    <dbReference type="eggNOG" id="COG1282">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007866_4_0_4"/>
    <dbReference type="OMA" id="AMTSMPE"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008750">
        <property type="term" value="F:NAD(P)+ transhydrogenase (AB-specific) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029035">
        <property type="entry name" value="DHS-like_NAD/FAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012136">
        <property type="entry name" value="NADH_DH_b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034300">
        <property type="entry name" value="PNTB-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02233">
        <property type="entry name" value="PNTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000204">
        <property type="entry name" value="PNTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52467">
        <property type="entry name" value="SSF52467"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 15" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="8 15" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="14 15 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-0520">NAD</keyword>
    <keyword evidence="11 15" id="KW-0521">NADP</keyword>
    <keyword evidence="18" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12 15" id="KW-1278">Translocase</keyword>
    <keyword evidence="10 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="13 16" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="6"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="36"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="76"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="106"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="149"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="191"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="216"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="domain" description="PNTB" evidence="17">
        <location>
            <begin position="10"/>
            <end position="487"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00003943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004429"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00007919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00014581"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRNR" id="PIRNR000204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF02233"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="ABF09838.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="490" mass="51213" checksum="428DB95737B5F4EF" modified="2006-05-30" version="1">MEVISMNLVTLLYLVASVCFIQALKGLSHPASARKGNAFGMIGMAIAVVTTLALIVKIKHEFLAVGAAQSSVGTGLALIFGALVVGGGIGAYVAKKVEMTKMPELVAAMHSLIGLAAVFIAVAAVAEPAAFGITPAGSHLIPLGNRIELFIGCFVGAITFSGSVIAFGKLSGRYKFRLFQGAPVVFPGQHWLNLLLAIGMIGFGVIFFLSQSWLPFLIMLAIAFVLGVLIIIPIGGADMPVVVSMLNSYSGWAAAGIGFSLNNPMLIIAGSLVGSSGAILSYIMCKAMNRSFFNVILGGFGADASAGATAGAQAQRNVKSGSADDAAFVMSNAETVIIVPGYGLAVARAQHALKELTEKLTEHGVTVKYAIHPVAGRMPGHMNVLLAEAEVPYDQVFEMEDINSEFGQADVVLVLGANDVVNPAAKTDPKSPIAGMPILEAYKAKTIIVNKRSMAAGYAGLDNELFYMDKTMMVFGDAKKVVEDMFKAVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LJ54</accession>
    <name>Q1LJ54_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Response regulaotr, CheY family belongs to the phcBSRQ operon that regulates phcA</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">phcQ</name>
        <name evidence="3" type="ordered locus">Rmet_2949</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09822.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517489.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2949"/>
    <dbReference type="EnsemblBacteria" id="ABF09822">
        <property type="protein sequence ID" value="ABF09822"/>
        <property type="gene ID" value="Rmet_2949"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2949"/>
    <dbReference type="eggNOG" id="COG3437">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_860124_0_0_4"/>
    <dbReference type="OMA" id="IKKPWDI"/>
    <dbReference type="OrthoDB" id="1755994at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039420">
        <property type="entry name" value="WalR-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48111">
        <property type="entry name" value="PTHR48111"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Response regulatory" evidence="2">
        <location>
            <begin position="12"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="1">
        <location>
            <position position="62"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF09822.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="332" mass="36144" checksum="CE7AD1C1E1B3EAEC" modified="2006-05-30" version="1">MSEPNTTQAPPAILFVDDEATAVKYFQRAIGQLAPVVTGGSVEEGKALLDAHGDSLAVLVSDQRMPGEYGNELLRYARERYPHIVRILTTAYSELDQTVEAVNQGQIHRYIKKPWDITALRMEMKQALELSGLRRERDQLVREKLMVLQKQTVATRVGMVHAICASLIGPGRFQPVETYLAGTSLAGARNAEPDWQRLDYADLVGAESQRCGVYGHAVGTKLAEFRARLAGRGAGDATAVLAEVLGAAVRRDGDVVTWAAPDALGEFVARPVADAVSDEHAAWLAALLWLEEAGGALQLQREGDAITARATTRTESFAKDRLAVWIEQLTEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LJC5</accession>
    <name>Q1LJC5_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Phosphoribosylglycinamide formyltransferase</fullName>
            <ecNumber evidence="2">2.1.2.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">5'-phosphoribosylglycinamide transformylase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="2">GAR transformylase</fullName>
            <shortName evidence="2">GART</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="2 4" type="primary">purN</name>
        <name evidence="4" type="ordered locus">Rmet_2878</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Catalyzes the transfer of a formyl group from 10-formyltetrahydrofolate to 5-phospho-ribosyl-glycinamide (GAR), producing 5-phospho-ribosyl-N-formylglycinamide (FGAR) and tetrahydrofolate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>(6S)-10-formyltetrahydrofolate + N(1)-(5-phospho-beta-D-ribosyl)glycinamide = (6S)-5,6,7,8-tetrahydrofolate + H(+) + N(2)-formyl-N(1)-(5-phospho-beta-D-ribosyl)glycinamide</text>
            <dbReference type="Rhea" id="RHEA:15053"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57453"/>
            <dbReference type="ChEBI" id="CHEBI:57454"/>
            <dbReference type="ChEBI" id="CHEBI:143788"/>
            <dbReference type="ChEBI" id="CHEBI:147286"/>
            <dbReference type="EC" id="2.1.2.2"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Purine metabolism; IMP biosynthesis via de novo pathway; N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide from N(1)-(5-phospho-D-ribosyl)glycinamide (10-formyl THF route): step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the GART family.</text>
    </comment>
    <dbReference type="EC" id="2.1.2.2" evidence="2"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09751.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517430.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2878"/>
    <dbReference type="EnsemblBacteria" id="ABF09751">
        <property type="protein sequence ID" value="ABF09751"/>
        <property type="gene ID" value="Rmet_2878"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2878"/>
    <dbReference type="eggNOG" id="COG0299">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038395_1_1_4"/>
    <dbReference type="OMA" id="TGITIHY"/>
    <dbReference type="OrthoDB" id="1815747at2"/>
    <dbReference type="UniPathway" id="UPA00074">
        <property type="reaction ID" value="UER00126"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004644">
        <property type="term" value="F:phosphoribosylglycinamide formyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd08645">
        <property type="entry name" value="FMT_core_GART"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01930">
        <property type="entry name" value="PurN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002376">
        <property type="entry name" value="Formyl_transf_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036477">
        <property type="entry name" value="Formyl_transf_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004607">
        <property type="entry name" value="GART"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00551">
        <property type="entry name" value="Formyl_trans_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53328">
        <property type="entry name" value="SSF53328"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00639">
        <property type="entry name" value="PurN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 2" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Formyl_trans_N" evidence="3">
        <location>
            <begin position="2"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="region of interest" description="5'-phosphoribosylglycinamide binding" evidence="2">
        <location>
            <begin position="12"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="region of interest" description="10-formyltetrahydrofolate binding" evidence="2">
        <location>
            <begin position="90"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="2">
        <location>
            <position position="109"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="65"/>
        </location>
        <ligand>
            <name>(6S)-10-formyltetrahydrofolate</name>
            <dbReference type="ChEBI" id="CHEBI:57454"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="107"/>
        </location>
        <ligand>
            <name>(6S)-10-formyltetrahydrofolate</name>
            <dbReference type="ChEBI" id="CHEBI:57454"/>
        </ligand>
    </feature>
    <feature type="site" description="Raises pKa of active site His" evidence="2">
        <location>
            <position position="145"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01930"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF09751.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="220" mass="23983" checksum="984A4AD8BA3D3B21" modified="2006-05-30" version="1">MKNIVILISGRGSNMEAIVRACAAEKWPARVAAVLSNRPDASGLQFASRHGIATGVVDHKQFSGRESFDAAMRDAIDAYQPDLIVLAGFMRILTPGFVEHYAGRMLNIHPSLLPSFPGLHTHKQALEAGVKLHGATVHFVTPELDHGPIVLQAALDVLPGDTPESLADRLLDSEHVIYPRAVRWFVEDRLHVQDGVVQVVQPAEPQWFMAISPQAAGKQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LK24</accession>
    <name>Q1LK24_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Cytokinin riboside 5'-monophosphate phosphoribohydrolase</fullName>
            <ecNumber evidence="2">3.2.2.n1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_2625</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>AMP + H2O = adenine + D-ribose 5-phosphate</text>
            <dbReference type="Rhea" id="RHEA:20129"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16708"/>
            <dbReference type="ChEBI" id="CHEBI:78346"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="3.2.2.4"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the LOG family.</text>
    </comment>
    <dbReference type="EC" id="3.2.2.n1" evidence="2"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09502.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008649826.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2625"/>
    <dbReference type="EnsemblBacteria" id="ABF09502">
        <property type="protein sequence ID" value="ABF09502"/>
        <property type="gene ID" value="Rmet_2625"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2625"/>
    <dbReference type="eggNOG" id="COG1611">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_058336_0_5_4"/>
    <dbReference type="OMA" id="MFVKYSQ"/>
    <dbReference type="OrthoDB" id="902542at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008714">
        <property type="term" value="F:AMP nucleosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009691">
        <property type="term" value="P:cytokinin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005269">
        <property type="entry name" value="LOG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031100">
        <property type="entry name" value="LOG_fam"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03641">
        <property type="entry name" value="Lysine_decarbox"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00730">
        <property type="entry name" value="TIGR00730"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0203">Cytokinin biosynthesis</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU363015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF09502.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="239" mass="26882" checksum="92EBFF994DF0E026" modified="2006-05-30" version="1">MIPSLRALADEDRATAKKARASWQMFTIMAEFIEATEYLSEIRPAVSIYGSARLREDSPYYQKTIEIARLFSDAGFAVISGGGPGIMEAANKGAHAGKSASVGLNIELPHEQQGNPYQDIAMRFRHFFTRKVTFVKNSDAFIVMPGGFGTLDELAEVLTLVQTGKSRSVPVVMYGSRFWKGLLDWFRFTLLPMGLIAEHDLDLMKIVDEPQEVLEAVYEYYEKRGGEHPIPPKEEMFYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="108" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LKD0</accession>
    <name>Q1LKD0_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">DNA topoisomerase 4 subunit A</fullName>
            <ecNumber evidence="5">5.6.2.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Topoisomerase IV subunit A</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5 8" type="primary">parC</name>
        <name evidence="8" type="ordered locus">Rmet_2519</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Topoisomerase IV is essential for chromosome segregation. It relaxes supercoiled DNA. Performs the decatenation events required during the replication of a circular DNA molecule.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 5">
            <text>ATP-dependent breakage, passage and rejoining of double-stranded DNA.</text>
            <dbReference type="EC" id="5.6.2.2"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="5">Heterotetramer composed of ParC and ParE.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cell membrane</location>
            <topology evidence="5">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the type II topoisomerase GyrA/ParC subunit family. ParC type 1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="5.6.2.2" evidence="5"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09396.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517095.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2519"/>
    <dbReference type="EnsemblBacteria" id="ABF09396">
        <property type="protein sequence ID" value="ABF09396"/>
        <property type="gene ID" value="Rmet_2519"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2519"/>
    <dbReference type="eggNOG" id="COG0188">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002977_4_1_4"/>
    <dbReference type="OMA" id="PRSNRID"/>
    <dbReference type="OrthoDB" id="217468at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019897">
        <property type="term" value="C:extrinsic component of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003918">
        <property type="term" value="F:DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007059">
        <property type="term" value="P:chromosome segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006265">
        <property type="term" value="P:DNA topological change"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00187">
        <property type="entry name" value="TOP4c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.268.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.199.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00936">
        <property type="entry name" value="ParC_type1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006691">
        <property type="entry name" value="GyrA/parC_rep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035516">
        <property type="entry name" value="Gyrase/topoIV_suA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013760">
        <property type="entry name" value="Topo_IIA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013758">
        <property type="entry name" value="Topo_IIA_A/C_ab"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013757">
        <property type="entry name" value="Topo_IIA_A_a_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002205">
        <property type="entry name" value="Topo_IIA_dom_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005742">
        <property type="entry name" value="TopoIV_A_Gneg"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03989">
        <property type="entry name" value="DNA_gyraseA_C"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00521">
        <property type="entry name" value="DNA_topoisoIV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00434">
        <property type="entry name" value="TOP4c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101904">
        <property type="entry name" value="SSF101904"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56719">
        <property type="entry name" value="SSF56719"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3 5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4 5 8" id="KW-0413">Isomerase</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0799">Topoisomerase</keyword>
    <feature type="domain" description="TOP4c" evidence="7">
        <location>
            <begin position="17"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="454"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="active site" description="O-(5'-phospho-DNA)-tyrosine intermediate" evidence="5">
        <location>
            <position position="128"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with DNA" evidence="5">
        <location>
            <position position="48"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with DNA" evidence="5">
        <location>
            <position position="84"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with DNA" evidence="5">
        <location>
            <position position="86"/>
        </location>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="5">
        <location>
            <position position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023029"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ABF09396.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="776" mass="84763" checksum="B3EEA7EE88B7C664" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="108" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LKF7</accession>
    <name>Q1LKF7_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Aconitate hydratase</fullName>
            <shortName evidence="12">Aconitase</shortName>
            <ecNumber evidence="12">4.2.1.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">acnA2</name>
        <name evidence="15" type="ordered locus">Rmet_2492</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Catalyzes the isomerization of citrate to isocitrate via cis-aconitate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11 12">
            <text>citrate = D-threo-isocitrate</text>
            <dbReference type="Rhea" id="RHEA:10336"/>
            <dbReference type="ChEBI" id="CHEBI:15562"/>
            <dbReference type="ChEBI" id="CHEBI:16947"/>
            <dbReference type="EC" id="4.2.1.3"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <text evidence="12">Binds 1 [4Fe-4S] cluster per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Carbohydrate metabolism; tricarboxylic acid cycle; isocitrate from oxaloacetate: step 2/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 12">Belongs to the aconitase/IPM isomerase family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.3" evidence="12"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09369.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517071.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2492"/>
    <dbReference type="EnsemblBacteria" id="ABF09369">
        <property type="protein sequence ID" value="ABF09369"/>
        <property type="gene ID" value="Rmet_2492"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2492"/>
    <dbReference type="eggNOG" id="COG1048">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013476_2_1_4"/>
    <dbReference type="OMA" id="NGGIMQY"/>
    <dbReference type="OrthoDB" id="363064at2"/>
    <dbReference type="UniPathway" id="UPA00223">
        <property type="reaction ID" value="UER00718"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003994">
        <property type="term" value="F:aconitate hydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047780">
        <property type="term" value="F:citrate dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009098">
        <property type="term" value="P:leucine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd01580">
        <property type="entry name" value="AcnA_IRP_Swivel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.19.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.499.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044137">
        <property type="entry name" value="AcnA_IRP_Swivel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015931">
        <property type="entry name" value="Acnase/IPM_dHydase_lsu_aba_1/3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001030">
        <property type="entry name" value="Acoase/IPM_deHydtase_lsu_aba"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015928">
        <property type="entry name" value="Aconitase/3IPM_dehydase_swvl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006249">
        <property type="entry name" value="Aconitase/IRP2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018136">
        <property type="entry name" value="Aconitase_4Fe-4S_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036008">
        <property type="entry name" value="Aconitase_4Fe-4S_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000573">
        <property type="entry name" value="AconitaseA/IPMdHydase_ssu_swvl"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11670">
        <property type="entry name" value="PTHR11670"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00330">
        <property type="entry name" value="Aconitase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00694">
        <property type="entry name" value="Aconitase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00415">
        <property type="entry name" value="ACONITASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53732">
        <property type="entry name" value="SSF53732"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01341">
        <property type="entry name" value="aconitase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00450">
        <property type="entry name" value="ACONITASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01244">
        <property type="entry name" value="ACONITASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="5" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="10" id="KW-0100">Branched-chain amino acid biosynthesis</keyword>
    <keyword evidence="7 12" id="KW-0408">Iron</keyword>
    <keyword evidence="8 12" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="4" id="KW-0432">Leucine biosynthesis</keyword>
    <keyword evidence="9 12 15" id="KW-0456">Lyase</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aconitase" evidence="13">
        <location>
            <begin position="70"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="domain" description="Aconitase_C" evidence="14">
        <location>
            <begin position="693"/>
            <end position="825"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004717"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU361275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="ABF09369.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="901" mass="97945" checksum="684172EB50F20396" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LKK9</accession>
    <name>Q1LKK9_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Ribonuclease E</fullName>
            <shortName evidence="5">RNase E</shortName>
            <ecNumber evidence="5">3.1.26.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5 8" type="primary">rne</name>
        <name evidence="8" type="ordered locus">Rmet_2440</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Endoribonuclease that plays a central role in RNA processing and decay. Required for the maturation of 5S and 16S rRNAs and the majority of tRNAs. Also involved in the degradation of most mRNAs.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>Endonucleolytic cleavage of single-stranded RNA in A- and U-rich regions.</text>
            <dbReference type="EC" id="3.1.26.12"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="5">Binds 1 Mg(2+) ion per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="5">Binds 2 Zn(2+) ions per homotetramer.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Homotetramer formed by a dimer of dimers.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Cell inner membrane</location>
            <topology evidence="5">Peripheral membrane protein</topology>
            <orientation evidence="5">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the RNase E/G family. RNase E subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.26.12" evidence="5"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09317.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517034.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="SMR" id="Q1LKK9"/>
    <dbReference type="STRING" id="266264.Rmet_2440"/>
    <dbReference type="EnsemblBacteria" id="ABF09317">
        <property type="protein sequence ID" value="ABF09317"/>
        <property type="gene ID" value="Rmet_2440"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2440"/>
    <dbReference type="eggNOG" id="COG1530">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003468_3_3_4"/>
    <dbReference type="OMA" id="MPQYASK"/>
    <dbReference type="OrthoDB" id="1209444at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009898">
        <property type="term" value="C:cytoplasmic side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004521">
        <property type="term" value="F:endoribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008995">
        <property type="term" value="F:ribonuclease E activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006402">
        <property type="term" value="P:mRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008033">
        <property type="term" value="P:tRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00970">
        <property type="entry name" value="RNase_E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019307">
        <property type="entry name" value="RNA-bd_AU-1/RNase_E/G"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028878">
        <property type="entry name" value="RNase_E"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004659">
        <property type="entry name" value="RNase_E/G"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022967">
        <property type="entry name" value="S1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003029">
        <property type="entry name" value="S1_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30001">
        <property type="entry name" value="PTHR30001"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10150">
        <property type="entry name" value="RNase_E_G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00575">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00316">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00757">
        <property type="entry name" value="RNaseEG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50126">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="2 5 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 5" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="1 5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0540">Nuclease</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="5" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="5" id="KW-0699">rRNA-binding</keyword>
    <keyword evidence="5" id="KW-0819">tRNA processing</keyword>
    <keyword evidence="5" id="KW-0820">tRNA-binding</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="S1 motif" evidence="7">
        <location>
            <begin position="39"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="region of interest" description="Required for zinc-mediated homotetramerization and catalytic activity" evidence="5">
        <location>
            <begin position="402"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="509"/>
            <end position="566"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="583"/>
            <end position="735"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="742"/>
            <end position="761"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="766"/>
            <end position="835"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="989"/>
            <end position="1018"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="513"/>
            <end position="529"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="600"/>
            <end position="679"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="695"/>
            <end position="729"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="301"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="344"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="402"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="405"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ABF09317.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="1037" mass="112882" checksum="AD3D5EFF82DA25CC" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="92" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LKL2</accession>
    <name>Q1LKL2_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Putative Rieske (2fe-2s) region harboring protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Rmet_2437</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09314.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517031.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2437"/>
    <dbReference type="EnsemblBacteria" id="ABF09314">
        <property type="protein sequence ID" value="ABF09314"/>
        <property type="gene ID" value="Rmet_2437"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2437"/>
    <dbReference type="eggNOG" id="COG2146">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_055690_4_2_4"/>
    <dbReference type="OMA" id="YLNQCAH"/>
    <dbReference type="OrthoDB" id="1632945at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019439">
        <property type="term" value="P:aromatic compound catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.102.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017941">
        <property type="entry name" value="Rieske_2Fe-2S"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036922">
        <property type="entry name" value="Rieske_2Fe-2S_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00355">
        <property type="entry name" value="Rieske"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50022">
        <property type="entry name" value="SSF50022"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51296">
        <property type="entry name" value="RIESKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0058">Aromatic hydrocarbons catabolism</keyword>
    <keyword evidence="3" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rieske" evidence="5">
        <location>
            <begin position="9"/>
            <end position="115"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022797"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABF09314.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="127" mass="13774" checksum="D2484F8EE74ED752" modified="2006-05-30" version="1">MPEASLEPRRLCAAEELVDGGAGVRFAVRLNTREIGAFVVRYDGAAHGYLNQCAHVPMELDWQEGQFFDSAGLYLMCATHGATYAPDTGLCVGGPCKGAALAKLRIEEREGAVFWLPEPPFFPLDAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="70" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LKV5</accession>
    <name>Q1LKV5_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative excisionase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_2344</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09221.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_003050245.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2344"/>
    <dbReference type="EnsemblBacteria" id="ABF09221">
        <property type="protein sequence ID" value="ABF09221"/>
        <property type="gene ID" value="Rmet_2344"/>
    </dbReference>
    <dbReference type="GeneID" id="56409852"/>
    <dbReference type="KEGG" id="rme:Rmet_2344"/>
    <dbReference type="eggNOG" id="COG3311">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_106726_1_0_4"/>
    <dbReference type="OMA" id="THRRVRY"/>
    <dbReference type="OrthoDB" id="1936441at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041657">
        <property type="entry name" value="HTH_17"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010093">
        <property type="entry name" value="SinI_DNA-bd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12728">
        <property type="entry name" value="HTH_17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01764">
        <property type="entry name" value="excise"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH_17" evidence="1">
        <location>
            <begin position="85"/>
            <end position="133"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF09221.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="156" mass="17246" checksum="AFA57A67943150A9" modified="2006-05-30" version="1">MTTATAQSKMTLPAAGEVKAAVQGQRALAAYLATQFETQHIQIFDDHKQAHQVELPTSALRLLVDILAELADGNAVKVVPVHAELTTQEAADLLNVSRPHFVKLLEDGVLAFHRTGKHRRVRFADLMQYKEARERASEQAMAELAQQSQELGMGYE</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LL20</accession>
    <name>Q1LL20_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="12">TonB-like protein, membrane spanning</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">tonB</name>
        <name evidence="12" type="ordered locus">Rmet_2277</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell inner membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
            <orientation evidence="1">Periplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TonB family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09156.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516982.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2277"/>
    <dbReference type="EnsemblBacteria" id="ABF09156">
        <property type="protein sequence ID" value="ABF09156"/>
        <property type="gene ID" value="Rmet_2277"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2277"/>
    <dbReference type="eggNOG" id="COG0810">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_076057_4_0_4"/>
    <dbReference type="OMA" id="ARTQPYN"/>
    <dbReference type="OrthoDB" id="1738032at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006260">
        <property type="entry name" value="TonB/TolA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037682">
        <property type="entry name" value="TonB_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03544">
        <property type="entry name" value="TonB_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01352">
        <property type="entry name" value="tonB_Cterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="6"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="domain" description="TonB_C" evidence="11">
        <location>
            <begin position="140"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="48"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="9">
        <location>
            <begin position="48"/>
            <end position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF03544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ABF09156.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="219" mass="23093" checksum="A3C5AC9770FF4295" modified="2006-05-30" version="1">MFDQRFFKITIAVLLFHAGLLYLIQSGLARKMTEAVIAPEIIARIIPMEPPQQPPAPEPPKQQPQTPPKQVKVVTPRPTPPKPQPTPTPVASLPPSPTAIEAPPAPPAPPAPAPEPAAPAAPANAAPRAVGIGEIACSPPQVTYPSQSRRMGETGKTVVRLTTDETGHVVKTAIVTSSGSTRLDTAAVNAVQAMRCKPYMDNGRAVAVTAQQPINFELN</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-01-15" modified="2022-05-25" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LLR7</accession>
    <name>RBFA_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Ribosome-binding factor A</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rbfA</name>
        <name type="ordered locus">Rmet_2030</name>
    </gene>
    <organism>
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">One of several proteins that assist in the late maturation steps of the functional core of the 30S ribosomal subunit. Associates with free 30S ribosomal subunits (but not with 30S subunits that are part of 70S ribosomes or polysomes). Required for efficient processing of 16S rRNA. May interact with the 5'-terminal helix region of 16S rRNA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Monomer. Binds 30S ribosomal subunits, but not 50S ribosomal subunits or 70S ribosomes.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the RbfA family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08909.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516745.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q1LLR7"/>
    <dbReference type="SMR" id="Q1LLR7"/>
    <dbReference type="STRING" id="266264.Rmet_2030"/>
    <dbReference type="EnsemblBacteria" id="ABF08909">
        <property type="protein sequence ID" value="ABF08909"/>
        <property type="gene ID" value="Rmet_2030"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2030"/>
    <dbReference type="eggNOG" id="COG0858">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_089475_5_1_4"/>
    <dbReference type="OMA" id="GDLQHCK"/>
    <dbReference type="OrthoDB" id="1971380at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030490">
        <property type="term" value="P:maturation of SSU-rRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00003">
        <property type="entry name" value="RbfA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015946">
        <property type="entry name" value="KH_dom-like_a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000238">
        <property type="entry name" value="RbfA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023799">
        <property type="entry name" value="RbfA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33515">
        <property type="entry name" value="PTHR33515"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02033">
        <property type="entry name" value="RBFA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89919">
        <property type="entry name" value="SSF89919"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00082">
        <property type="entry name" value="rbfA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0690">Ribosome biogenesis</keyword>
    <feature type="chain" id="PRO_1000000185" description="Ribosome-binding factor A">
        <location>
            <begin position="1"/>
            <end position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00003"/>
        </source>
    </evidence>
    <sequence length="123" mass="13895" checksum="1E35C834E02236E7" modified="2006-05-30" version="1">MAKKGNITSRNLRLSDQIQKELAEMIQREIRDPRLGLVTLQSVSLTPDYAHAKVYFTVLGAEPETAAAILKEKAGYLHSLLFKRLHIHTVPTLHFHHDTSVEHAIEMSKLINEANATRAKDDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="116" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LLZ2</accession>
    <name>Q1LLZ2_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Transcription-repair-coupling factor</fullName>
            <shortName evidence="8">TRCF</shortName>
            <ecNumber evidence="8">3.6.4.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8 11" type="primary">mfd</name>
        <name evidence="11" type="ordered locus">Rmet_1955</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Couples transcription and DNA repair by recognizing RNA polymerase (RNAP) stalled at DNA lesions. Mediates ATP-dependent release of RNAP and its truncated transcript from the DNA, and recruitment of nucleotide excision repair machinery to the damaged site.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">In the C-terminal section; belongs to the helicase family. RecG subfamily.</text>
    </comment>
    <comment type="similarity">
        <text evidence="8">In the N-terminal section; belongs to the UvrB family.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.-" evidence="8"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08834.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008651552.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1955"/>
    <dbReference type="EnsemblBacteria" id="ABF08834">
        <property type="protein sequence ID" value="ABF08834"/>
        <property type="gene ID" value="Rmet_1955"/>
    </dbReference>
    <dbReference type="GeneID" id="60821439"/>
    <dbReference type="KEGG" id="rme:Rmet_1955"/>
    <dbReference type="eggNOG" id="COG1197">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005122_1_3_4"/>
    <dbReference type="OMA" id="WAPPCRE"/>
    <dbReference type="OrthoDB" id="234717at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009380">
        <property type="term" value="C:excinuclease repair complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003684">
        <property type="term" value="F:damaged DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000716">
        <property type="term" value="P:transcription-coupled nucleotide-excision repair, DNA damage recognition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00969">
        <property type="entry name" value="TRCF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036101">
        <property type="entry name" value="CarD-like/TRCF_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003711">
        <property type="entry name" value="CarD-like/TRCF_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004576">
        <property type="entry name" value="Mfd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037235">
        <property type="entry name" value="TRCF-like_C_D7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005118">
        <property type="entry name" value="TRCF_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004807">
        <property type="entry name" value="UvrB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041471">
        <property type="entry name" value="UvrB_inter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24029">
        <property type="entry name" value="PTHR24029"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02559">
        <property type="entry name" value="CarD_CdnL_TRCF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03461">
        <property type="entry name" value="TRCF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17757">
        <property type="entry name" value="UvrB_inter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01058">
        <property type="entry name" value="CarD_TRCF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00982">
        <property type="entry name" value="TRCF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF141259">
        <property type="entry name" value="SSF141259"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143517">
        <property type="entry name" value="SSF143517"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00580">
        <property type="entry name" value="mfd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 8" id="KW-0227">DNA damage</keyword>
    <keyword evidence="7 8" id="KW-0234">DNA repair</keyword>
    <keyword evidence="6 8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0347">Helicase</keyword>
    <keyword evidence="3 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="9">
        <location>
            <begin position="623"/>
            <end position="784"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="10">
        <location>
            <begin position="805"/>
            <end position="959"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00969"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ABF08834.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="1150" mass="127872" checksum="8FF4725413E84C19" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="100" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LM17</accession>
    <name>Q1LM17_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Transcriptional regulator, IclR-family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Rmet_1930</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08809.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516661.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1930"/>
    <dbReference type="EnsemblBacteria" id="ABF08809">
        <property type="protein sequence ID" value="ABF08809"/>
        <property type="gene ID" value="Rmet_1930"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1930"/>
    <dbReference type="eggNOG" id="COG1414">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_062618_0_1_4"/>
    <dbReference type="OMA" id="RYPVRMY"/>
    <dbReference type="OrthoDB" id="370329at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029016">
        <property type="entry name" value="GAF-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014757">
        <property type="entry name" value="Tscrpt_reg_IclR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005471">
        <property type="entry name" value="Tscrpt_reg_IclR_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09339">
        <property type="entry name" value="HTH_IclR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01614">
        <property type="entry name" value="IclR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00346">
        <property type="entry name" value="HTH_ICLR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51077">
        <property type="entry name" value="HTH_ICLR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51078">
        <property type="entry name" value="ICLR_ED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH iclR-type" evidence="3">
        <location>
            <begin position="14"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="domain" description="IclR-ED" evidence="4">
        <location>
            <begin position="77"/>
            <end position="261"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABF08809.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="261" mass="28599" checksum="1725321FAA080D0B" modified="2006-05-30" version="1">MTTDDKERPDPLFNQSLEKGLEVLRAFSAVHRTLTLAELAALTGMTKSSAQRTIHTLERLGYVDKHPQTRRFRLTPRVMEIGYNYLAADALIPIASPYLSQLANASGETANLTEPVGLEMVYMAQLVTAKHMPVLTPVGMRIPMYCTSSGRAYLSTLPDERVRAMLEASARTPRTPVTLTDVETIFERVVACRTVGYACNEEELFLGDMGIAAPVVNSRGEAVGAVHVSPPASRWTMVDAQKKLGPLVVECAWAISRSIAH</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LM34</accession>
    <name>Q1LM34_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">ABM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_1913</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08792.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516646.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1913"/>
    <dbReference type="EnsemblBacteria" id="ABF08792">
        <property type="protein sequence ID" value="ABF08792"/>
        <property type="gene ID" value="Rmet_1913"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1913"/>
    <dbReference type="eggNOG" id="COG3224">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_075307_0_0_4"/>
    <dbReference type="OMA" id="VWLWMPI"/>
    <dbReference type="OrthoDB" id="1695606at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007138">
        <property type="entry name" value="ABM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038762">
        <property type="entry name" value="ABM_predict"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011008">
        <property type="entry name" value="Dimeric_a/b-barrel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40057">
        <property type="entry name" value="PTHR40057"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03992">
        <property type="entry name" value="ABM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54909">
        <property type="entry name" value="SSF54909"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="125"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="159"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="domain" description="ABM" evidence="2">
        <location>
            <begin position="12"/>
            <end position="84"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF03992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF08792.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="188" mass="21460" checksum="0EC0812885DD9468" modified="2006-05-30" version="1">MNTTTLNPQDIVTSIIVHEVEPGAAGQYETWLADIRVACSRFPGYLSTDVIRPVGKSREYATIVRFADFKSLNAWMESDERRQHIARMQSSLRQGDRYEIRTGLDFWFTPKHAPVKVPTAWKQWLVTWSAILPLSIVVPLGVVPMLARLGFARDSFADKAVVSAAIVFLMVYVVMPRYTRLVARWLFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="63" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LM45</accession>
    <name>Q1LM45_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_1902</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08781.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516635.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1902"/>
    <dbReference type="EnsemblBacteria" id="ABF08781">
        <property type="protein sequence ID" value="ABF08781"/>
        <property type="gene ID" value="Rmet_1902"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1902"/>
    <dbReference type="eggNOG" id="ENOG502Z8S1">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_086940_1_0_4"/>
    <dbReference type="OMA" id="DNVNINY"/>
    <dbReference type="OrthoDB" id="1342393at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024453">
        <property type="entry name" value="Peptidase_C92"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05708">
        <property type="entry name" value="Peptidase_C92"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="SSF54001"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF08781.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="209" mass="23050" checksum="8B0C51B9C57D1E2F" modified="2006-05-30" version="1">MQIDSNVLDLATTLQVGDIVFIRVRARPFREVATATASWTNHVGIVVANSGTGTSIAESTFPLSRTTSLKRFAARSEHGRIAIARLRQPLTASQQLRLSEAVRHRMGIWYDTGFNLHSRRQFCSRFVREVLAESADIGVGEAETFKALLARQPDAALGFWRLWYFGRIPWERETVTPASLLCSPALELLFDGTVQPAHAAANRSGGAQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LMC4</accession>
    <name>Q1LMC4_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">J domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_1823</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08702.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516552.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1823"/>
    <dbReference type="EnsemblBacteria" id="ABF08702">
        <property type="protein sequence ID" value="ABF08702"/>
        <property type="gene ID" value="Rmet_1823"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1823"/>
    <dbReference type="eggNOG" id="COG0484">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_049164_1_0_4"/>
    <dbReference type="OMA" id="QRTPTHY"/>
    <dbReference type="OrthoDB" id="1063977at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00625">
        <property type="entry name" value="JDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="SSF46565"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="J" evidence="2">
        <location>
            <begin position="3"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="74"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="128"/>
            <end position="145"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF08702.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="345" mass="37320" checksum="9DC6E20969653D0D" modified="2006-05-30" version="1">MHTHYDNLKVSRDAPAEVIRAAYKSLSQKYHPDRHADSEQANRIMRLLNAAYDVLSDPAKRAEHDQWIASQEKLDAERRQRQAQQAAASRTAAQPPRPQGRPARPAQSAQASARATGAAAPGQSAADSAQKKKRRKPRQDGQRPGEPLARKWSLLLYAGIGAAIVVLLGDWATPTQPAVDKPGAALVQPAAAASIVTPVPADLPREAQYLRPSLTPRGLPWPTNSGYLPGYPVLNTSGLSTLTVDNSRGPSDVLLKLVAVNGGIPVPVRTTFIPVGSSFTMDAITQGSYDVRYQSLDTGEIYRSERFVFEEMREAGSTSAGRNVLTLYAAPGKSRSVTITEREFP</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="63" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LMJ3</accession>
    <name>Q1LMJ3_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_1754</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
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    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
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    </dbReference>
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        <source>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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</entry>
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        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
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            <dbReference type="PubMed" id="20463976"/>
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        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="102" xmlns="http://uniprot.org/uniprot">
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            <fullName evidence="5">DNA polymerase</fullName>
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        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
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                <person name="Van Houdt R."/>
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                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
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                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
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                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <text>a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) = diphosphate + DNA(n+1)</text>
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        <text evidence="1 5">Belongs to the DNA polymerase type-B family.</text>
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        <source>
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        <source>
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        <source>
            <dbReference type="Pfam" id="PF03104"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="ABF08528.1"/>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="96" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LMV7</accession>
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        </submittedName>
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        <name type="synonym">Ralstonia metallidurans</name>
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        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the TPP enzyme family.</text>
    </comment>
    <dbReference type="EC" id="2.2.1.6" evidence="6"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08519.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516364.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1636"/>
    <dbReference type="EnsemblBacteria" id="ABF08519">
        <property type="protein sequence ID" value="ABF08519"/>
        <property type="gene ID" value="Rmet_1636"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1636"/>
    <dbReference type="eggNOG" id="COG0028">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013748_3_4_4"/>
    <dbReference type="OMA" id="GFPIKPQ"/>
    <dbReference type="OrthoDB" id="391134at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003984">
        <property type="term" value="F:acetolactate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030976">
        <property type="term" value="F:thiamine pyrophosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044281">
        <property type="term" value="P:small molecule metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029035">
        <property type="entry name" value="DHS-like_NAD/FAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012000">
        <property type="entry name" value="Thiamin_PyroP_enz_cen_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012001">
        <property type="entry name" value="Thiamin_PyroP_enz_TPP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000399">
        <property type="entry name" value="TPP-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045229">
        <property type="entry name" value="TPP_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011766">
        <property type="entry name" value="TPP_enzyme-bd_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18968">
        <property type="entry name" value="PTHR18968"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02775">
        <property type="entry name" value="TPP_enzyme_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00205">
        <property type="entry name" value="TPP_enzyme_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02776">
        <property type="entry name" value="TPP_enzyme_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52467">
        <property type="entry name" value="SSF52467"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="SSF52518"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00187">
        <property type="entry name" value="TPP_ENZYMES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0786">Thiamine pyrophosphate</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="TPP_enzyme_N" evidence="5">
        <location>
            <begin position="12"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="domain" description="TPP_enzyme_M" evidence="3">
        <location>
            <begin position="198"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="domain" description="TPP_enzyme_C" evidence="4">
        <location>
            <begin position="395"/>
            <end position="540"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007812"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU362132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF02775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF02776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABF08519.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="559" mass="59935" checksum="4358A9C225C2EE21" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="112" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LMW7</accession>
    <name>Q1LMW7_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Sensor protein QseC</fullName>
            <ecNumber evidence="4">2.7.13.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ordered locus">Rmet_1626</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.</text>
            <dbReference type="EC" id="2.7.13.3"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell inner membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.7.13.3" evidence="4"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08509.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516355.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1626"/>
    <dbReference type="EnsemblBacteria" id="ABF08509">
        <property type="protein sequence ID" value="ABF08509"/>
        <property type="gene ID" value="Rmet_1626"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1626"/>
    <dbReference type="eggNOG" id="COG2205">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_89_37_4"/>
    <dbReference type="OMA" id="SFERNMN"/>
    <dbReference type="OrthoDB" id="692375at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00082">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013727">
        <property type="entry name" value="2CSK_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003660">
        <property type="entry name" value="HAMP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003661">
        <property type="entry name" value="HisK_dim/P"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036097">
        <property type="entry name" value="HisK_dim/P_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004358">
        <property type="entry name" value="Sig_transdc_His_kin-like_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08521">
        <property type="entry name" value="2CSK_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00512">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00344">
        <property type="entry name" value="BCTRLSENSOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00388">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47384">
        <property type="entry name" value="SSF47384"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="SSF55874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50885">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9 16" id="KW-0418">Kinase</keyword>
    <keyword evidence="11 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 16" id="KW-0808">Transferase</keyword>
    <keyword evidence="8 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="164"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="domain" description="HAMP" evidence="15">
        <location>
            <begin position="188"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="14">
        <location>
            <begin position="248"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="459"/>
            <end position="478"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004429"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00017234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="ABF08509.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="478" mass="51991" checksum="68D94555E8742E63" modified="2006-05-30" version="1">MKTGSLRLQITLWLLIPLLGLLAFDAWLTDRRAMSAAHQAFDRTLAASLKSMREGISLRDGRVEVEIPNLALEVFDAEDGSRVYYQIRDERGTTITGYEGLPMPEGPVNSLYRTQFYDAEYLGVPVRMAAMPLPLHDVRSARTQMVWVLLAESTEPREQLASDILVGSLLQELMLISLALGIVWLGVRRGLLPLQRLSDAMSARGPDDLEPLEDTALPVEMKPLVAAVNQYVARLHRMIEGRKRFFADAAHQLKTPLAIIQAESELALREKDLPSVRAHVERLHGSVQQAAKEVAQLLSLSRLEPDSGYAPTLRHLRLDTLAQNVALEWAPLARRAGVDLGFEGRGPADIDGQTELLQELLGNLIDNAIRYAGAGAMVTVRVDGQRLAVEDNGPGVPAFERESIFKRFYRGESSASRDGSGLGLSIVREIARIHGAAVALESTPGGGLTVVVTFPAPALASGPGPASTDSPGRAAESR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="97" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LMZ5</accession>
    <name>Q1LMZ5_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">ABC-type transporter: periplasmic glycine betaine/choline-binding lipoprotein and permease components transmembrane</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Rmet_1598</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cell membrane</location>
            <topology evidence="5">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the binding-protein-dependent transport system permease family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08481.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516328.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1598"/>
    <dbReference type="EnsemblBacteria" id="ABF08481">
        <property type="protein sequence ID" value="ABF08481"/>
        <property type="gene ID" value="Rmet_1598"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1598"/>
    <dbReference type="eggNOG" id="COG1174">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG1732">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_577198_0_0_4"/>
    <dbReference type="OMA" id="FPRYDAV"/>
    <dbReference type="OrthoDB" id="770506at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043190">
        <property type="term" value="C:ATP-binding cassette (ABC) transporter complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007210">
        <property type="entry name" value="ABC_Gly_betaine_transp_sub-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04069">
        <property type="entry name" value="OpuAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="SSF161098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0732">Signal</keyword>
    <keyword evidence="1 5 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193350" evidence="6">
        <location>
            <begin position="34"/>
            <end position="516"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="322"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="353"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="379"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="445"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="484"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="7">
        <location>
            <begin position="318"/>
            <end position="502"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ABF08481.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="516" mass="54426" checksum="A53A6758A83E8133" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LNV2</accession>
    <name>Q1LNV2_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Rubredoxin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">hoxR</name>
        <name evidence="8" type="ordered locus">Rmet_1289</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 6">
            <name>Fe(3+)</name>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the rubredoxin family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08174.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516056.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1289"/>
    <dbReference type="EnsemblBacteria" id="ABF08174">
        <property type="protein sequence ID" value="ABF08174"/>
        <property type="gene ID" value="Rmet_1289"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1289"/>
    <dbReference type="eggNOG" id="COG1773">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_128747_2_1_4"/>
    <dbReference type="OMA" id="ECKICWW"/>
    <dbReference type="OrthoDB" id="2047418at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00730">
        <property type="entry name" value="rubredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024934">
        <property type="entry name" value="Rubredoxin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024935">
        <property type="entry name" value="Rubredoxin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018527">
        <property type="entry name" value="Rubredoxin_Fe_BS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00301">
        <property type="entry name" value="Rubredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00163">
        <property type="entry name" value="RUBREDOXIN"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00202">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50903">
        <property type="entry name" value="RUBREDOXIN_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0249">Electron transport</keyword>
    <keyword evidence="5 6" id="KW-0408">Iron</keyword>
    <keyword evidence="3 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Rubredoxin-like" evidence="7">
        <location>
            <begin position="23"/>
            <end position="74"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001965"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003820"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50903"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ABF08174.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="76" mass="8713" checksum="8B0C051CC67F5A50" modified="2006-05-30" version="1">MNGDRFEGFEGSYLGNRDVLKPGSRLECKICWWVYDPAVGDPQWQIPPGTPFAELPPHWRCPNCDCDAEQFLLLND</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LNY3</accession>
    <name>Q1LNY3_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Plasmid conjugal transfer transmembrane protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">traF</name>
        <name evidence="2" type="ordered locus">Rmet_1258</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08143.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516030.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1258"/>
    <dbReference type="MEROPS" id="S26.014"/>
    <dbReference type="EnsemblBacteria" id="ABF08143">
        <property type="protein sequence ID" value="ABF08143"/>
        <property type="gene ID" value="Rmet_1258"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1258"/>
    <dbReference type="eggNOG" id="COG4959">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_104604_0_0_4"/>
    <dbReference type="OMA" id="WVINKRE"/>
    <dbReference type="OrthoDB" id="1741894at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006465">
        <property type="term" value="P:signal peptide processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036286">
        <property type="entry name" value="LexA/Signal_pep-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019533">
        <property type="entry name" value="Peptidase_S26"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10502">
        <property type="entry name" value="Peptidase_S26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51306">
        <property type="entry name" value="SSF51306"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <feature type="domain" description="Peptidase_S26" evidence="1">
        <location>
            <begin position="21"/>
            <end position="190"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF10502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF08143.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="195" mass="20895" checksum="02D7BE96143420AA" modified="2006-05-30" version="1">MTAVSTASTAPRPRSRLRARLVLAGLSACGLAALAWASFVHPLPRLIYNPSDSVAVGWYRVDPLHHGTASLPRPLSVGSIVLTTLPPDAAVLAAQRGYLPTRVPLLKRVGAVAPQEVCITGRVVRIDGVLSAAVLPADRWGRPLPSWQQCRRLEPGELFLLSVTNQASFDSRYFGPVSTSAVIGVAHPVWLESRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="97" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LP10</accession>
    <name>Q1LP10_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Leucine/isoleucine/valine transporter subunit, ATP-binding component of ABC superfamily</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">livF6</name>
        <name evidence="9" type="ordered locus">Rmet_1230</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08116.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008646845.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1230"/>
    <dbReference type="EnsemblBacteria" id="ABF08116">
        <property type="protein sequence ID" value="ABF08116"/>
        <property type="gene ID" value="Rmet_1230"/>
    </dbReference>
    <dbReference type="GeneID" id="60822102"/>
    <dbReference type="KEGG" id="rme:Rmet_1230"/>
    <dbReference type="eggNOG" id="COG0410">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_2_4"/>
    <dbReference type="OMA" id="RVICMLE"/>
    <dbReference type="OrthoDB" id="1475841at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 7 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="1" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-1278">Translocase</keyword>
    <feature type="domain" description="ABC transporter" evidence="8">
        <location>
            <begin position="7"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="39"/>
            <end position="46"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ABF08116.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="238" mass="25593" checksum="99AD5253476E0776" modified="2006-05-30" version="1">MSHAAIIDADGVNAWYGSSHVLRGIDFRVGAGESVGLLGRNGMGKSTLLRTLLGHVREREGGIRVVGRDMSRARPHEVARLGIAYVPEGRGIFPNLSVRENLLMAARAGSQGRKDWDEARVLDLFPRLKERLSHGGQQLSGGEQQMLSIGRALMTNPDCLILDEATEGLAPKIVHEIWNVIATIRATGIASVVVDRNYRAVLAHVDRVVVLEKGLVVMSGPAADLAAAPAALDRYLGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="62" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LP21</accession>
    <name>Q1LP21_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF2384 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_1219</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08105.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515994.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1219"/>
    <dbReference type="EnsemblBacteria" id="ABF08105">
        <property type="protein sequence ID" value="ABF08105"/>
        <property type="gene ID" value="Rmet_1219"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1219"/>
    <dbReference type="eggNOG" id="COG5642">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_157727_0_0_4"/>
    <dbReference type="OMA" id="HRKEWEF"/>
    <dbReference type="OrthoDB" id="1850771at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024467">
        <property type="entry name" value="Xre/MbcA/ParS-like_toxin-bd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09722">
        <property type="entry name" value="DUF2384"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF2384" evidence="1">
        <location>
            <begin position="79"/>
            <end position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF09722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF08105.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="129" mass="13949" checksum="4E573023BCCAE925" modified="2006-05-30" version="1">MQSGQIPTPDPGSAPDPGVTLTKAVMRAASFLGISQAIVASVLGISTASVSRMASGGYVLDVHRKEWEFAVLFVRLFRSLDAILGHEEQARLWLTHDNLALGGKPLELIRTTEGIVRVVHYLDATRGRI</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-12-12" modified="2022-05-25" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LP26</accession>
    <name>BPT_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Aspartate/glutamate leucyltransferase</fullName>
            <ecNumber evidence="1">2.3.2.29</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">bpt</name>
        <name type="ordered locus">Rmet_1214</name>
    </gene>
    <organism>
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Functions in the N-end rule pathway of protein degradation where it conjugates Leu from its aminoacyl-tRNA to the N-termini of proteins containing an N-terminal aspartate or glutamate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>L-leucyl-tRNA(Leu) + N-terminal L-glutamyl-[protein] = H(+) + N-terminal L-leucyl-L-glutamyl-[protein] + tRNA(Leu)</text>
            <dbReference type="Rhea" id="RHEA:50412"/>
            <dbReference type="Rhea" id="RHEA-COMP:9613"/>
            <dbReference type="Rhea" id="RHEA-COMP:9622"/>
            <dbReference type="Rhea" id="RHEA-COMP:12664"/>
            <dbReference type="Rhea" id="RHEA-COMP:12668"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:64721"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78494"/>
            <dbReference type="ChEBI" id="CHEBI:133041"/>
            <dbReference type="EC" id="2.3.2.29"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>L-leucyl-tRNA(Leu) + N-terminal L-aspartyl-[protein] = H(+) + N-terminal L-leucyl-L-aspartyl-[protein] + tRNA(Leu)</text>
            <dbReference type="Rhea" id="RHEA:50420"/>
            <dbReference type="Rhea" id="RHEA-COMP:9613"/>
            <dbReference type="Rhea" id="RHEA-COMP:9622"/>
            <dbReference type="Rhea" id="RHEA-COMP:12669"/>
            <dbReference type="Rhea" id="RHEA-COMP:12674"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:64720"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78494"/>
            <dbReference type="ChEBI" id="CHEBI:133042"/>
            <dbReference type="EC" id="2.3.2.29"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the R-transferase family. Bpt subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.29" evidence="1"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08100.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515990.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q1LP26"/>
    <dbReference type="STRING" id="266264.Rmet_1214"/>
    <dbReference type="EnsemblBacteria" id="ABF08100">
        <property type="protein sequence ID" value="ABF08100"/>
        <property type="gene ID" value="Rmet_1214"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1214"/>
    <dbReference type="eggNOG" id="COG2935">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_077607_0_0_4"/>
    <dbReference type="OMA" id="MVEDSHV"/>
    <dbReference type="OrthoDB" id="1675133at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004057">
        <property type="term" value="F:arginyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008914">
        <property type="term" value="F:leucyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071596">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process via the N-end rule pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00689">
        <property type="entry name" value="Bpt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017138">
        <property type="entry name" value="Asp_Glu_LeuTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030700">
        <property type="entry name" value="N-end_Aminoacyl_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007472">
        <property type="entry name" value="N-end_Aminoacyl_Trfase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007471">
        <property type="entry name" value="N-end_Aminoacyl_Trfase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21367">
        <property type="entry name" value="PTHR21367"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04377">
        <property type="entry name" value="ATE_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04376">
        <property type="entry name" value="ATE_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037208">
        <property type="entry name" value="ATE_pro_prd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="SSF55729"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0012">Acyltransferase</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000263206" description="Aspartate/glutamate leucyltransferase">
        <location>
            <begin position="1"/>
            <end position="262"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00689"/>
        </source>
    </evidence>
    <sequence length="262" mass="29961" checksum="C25FAF6511759B60" modified="2006-05-30" version="1">MSKLKELPLSALQFYATAPYACSYLEGRMARSQVATPAHLINADVYSRLVRAGFRRSGIFTYRPYCDECRACTPCRVLVDQFRPDRSQRRAWRDHQGLQALVAPLTYVEEHYALYLLYQSMRHAGGGMDQDSRDQYEQFLLQSRVNSRLVEFREPPGSPEAGRLRMVSMIDVLDDGLSSVYTFYDPLIVGASYGTYNILWQINQTRELGLPHLYLGYWIADSRKMAYKARFQPLQVLTGNQWHTFKAPAEASGQPAPDPALE</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="105" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LP95</accession>
    <name>Q1LP95_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Trimethylamine-N-oxide reductase</fullName>
            <ecNumber evidence="4">1.7.2.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">torA</name>
        <name evidence="11" type="ordered locus">Rmet_1145</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mo-bis(molybdopterin guanine dinucleotide)</name>
            <dbReference type="ChEBI" id="CHEBI:60539"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Periplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the prokaryotic molybdopterin-containing oxidoreductase family.</text>
    </comment>
    <dbReference type="EC" id="1.7.2.3" evidence="4"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08031.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515933.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1145"/>
    <dbReference type="EnsemblBacteria" id="ABF08031">
        <property type="protein sequence ID" value="ABF08031"/>
        <property type="gene ID" value="Rmet_1145"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1145"/>
    <dbReference type="eggNOG" id="COG0243">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000422_13_3_4"/>
    <dbReference type="OMA" id="KPWVRHA"/>
    <dbReference type="OrthoDB" id="88184at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042597">
        <property type="term" value="C:periplasmic space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043546">
        <property type="term" value="F:molybdopterin cofactor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050626">
        <property type="term" value="F:trimethylamine-N-oxide reductase (cytochrome c) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd02793">
        <property type="entry name" value="MopB_CT_DMSOR-BSOR-TMAOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009010">
        <property type="entry name" value="Asp_de-COase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006658">
        <property type="entry name" value="BisC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041954">
        <property type="entry name" value="CT_DMSOR/BSOR/TMAOR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041460">
        <property type="entry name" value="Molybdopterin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006657">
        <property type="entry name" value="MoPterin_dinucl-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006656">
        <property type="entry name" value="Mopterin_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006655">
        <property type="entry name" value="Mopterin_OxRdtase_prok_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006311">
        <property type="entry name" value="TAT_signal"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00384">
        <property type="entry name" value="Molybdopterin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18364">
        <property type="entry name" value="Molybdopterin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01568">
        <property type="entry name" value="Molydop_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50692">
        <property type="entry name" value="SSF50692"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00509">
        <property type="entry name" value="bisC_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00490">
        <property type="entry name" value="MOLYBDOPTERIN_PROK_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00932">
        <property type="entry name" value="MOLYBDOPTERIN_PROK_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51318">
        <property type="entry name" value="TAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Molybdopterin_N" evidence="10">
        <location>
            <begin position="51"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="domain" description="Molybdopterin" evidence="8">
        <location>
            <begin position="95"/>
            <end position="562"/>
        </location>
    </feature>
    <feature type="domain" description="Molydop_binding" evidence="9">
        <location>
            <begin position="684"/>
            <end position="798"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010312"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF18364"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ABF08031.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="825" mass="91155" checksum="060D8340BD2F39A1" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-02-10" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LPE4</accession>
    <name>Q1LPE4_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Glyceraldehyde 3-phosphate dehydrogenase</fullName>
            <ecNumber evidence="6">1.2.1.12</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Rmet_1096</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell outer membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="1.2.1.12" evidence="6"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07982.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1096"/>
    <dbReference type="EnsemblBacteria" id="ABF07982">
        <property type="protein sequence ID" value="ABF07982"/>
        <property type="gene ID" value="Rmet_1096"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1096"/>
    <dbReference type="eggNOG" id="COG4775">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046092_2_0_4"/>
    <dbReference type="OMA" id="DASQWIL"/>
    <dbReference type="OrthoDB" id="364966at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009279">
        <property type="term" value="C:cell outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004365">
        <property type="term" value="F:glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000184">
        <property type="entry name" value="Bac_surfAg_D15"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039910">
        <property type="entry name" value="D15-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12815">
        <property type="entry name" value="PTHR12815"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01103">
        <property type="entry name" value="Omp85"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1134">Transmembrane beta strand</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193396" evidence="4">
        <location>
            <begin position="30"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="domain" description="Omp85" evidence="5">
        <location>
            <begin position="199"/>
            <end position="279"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004442"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABF07982.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="383" mass="42354" checksum="99F01665B41622C1" modified="2006-05-30" version="1">MRGIAFRRSSLLLAAISLLLPFIPICASAQSSSPSFRDPEDGAFDLSNYLLEHKGALPVPFVITEPAVGYGVGIGLLFFSESIAESEARAKELGQERVPPNITVLGGAYTENGTWAGVVGHFHSWDNDRYRYLGGVAKVDANLDYFGVANQARAYELDGVGLFQQLLVRIGNSRWFAGPRYIFFDANAIFKFGQNPSDVTGQGRDQRISAASLVIDYDSRDNIFFPNRGSFAEIETQFARDGIGSTQNYNMYAARGYTWLPLGKSFVLGLRADTRFSTGDVPFFVQPYIDLRGVKKARYQDRNAIATEVELRWDIKPRWSVLGFSGLGKAYGRQSFSDAENVVSVGMGFRYLIARRLGLAMGLDVAHSKDQNAIYVQVGTAWH</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-02-05" modified="2022-05-25" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LPT0</accession>
    <name>URE2_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Urease subunit beta</fullName>
            <ecNumber evidence="1">3.5.1.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Urea amidohydrolase subunit beta</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">ureB</name>
        <name type="ordered locus">Rmet_0960</name>
    </gene>
    <organism>
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>2 H(+) + H2O + urea = CO2 + 2 NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:20557"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16199"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="EC" id="3.5.1.5"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Nitrogen metabolism; urea degradation; CO(2) and NH(3) from urea (urease route): step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Heterotrimer of UreA (gamma), UreB (beta) and UreC (alpha) subunits. Three heterotrimers associate to form the active enzyme.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the urease beta subunit family.</text>
    </comment>
    <dbReference type="EC" id="3.5.1.5" evidence="1"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07846.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515768.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q1LPT0"/>
    <dbReference type="SMR" id="Q1LPT0"/>
    <dbReference type="STRING" id="266264.Rmet_0960"/>
    <dbReference type="EnsemblBacteria" id="ABF07846">
        <property type="protein sequence ID" value="ABF07846"/>
        <property type="gene ID" value="Rmet_0960"/>
    </dbReference>
    <dbReference type="GeneID" id="60825447"/>
    <dbReference type="KEGG" id="rme:Rmet_0960"/>
    <dbReference type="eggNOG" id="COG0832">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_129707_1_1_4"/>
    <dbReference type="OMA" id="FYEVNDA"/>
    <dbReference type="OrthoDB" id="1755809at2"/>
    <dbReference type="UniPathway" id="UPA00258">
        <property type="reaction ID" value="UER00370"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035550">
        <property type="term" value="C:urease complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009039">
        <property type="term" value="F:urease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043419">
        <property type="term" value="P:urea catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00407">
        <property type="entry name" value="Urease_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.150.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01954">
        <property type="entry name" value="Urease_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002019">
        <property type="entry name" value="Urease_beta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036461">
        <property type="entry name" value="Urease_betasu_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00699">
        <property type="entry name" value="Urease_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51278">
        <property type="entry name" value="SSF51278"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00192">
        <property type="entry name" value="urease_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_1000070765" description="Urease subunit beta">
        <location>
            <begin position="1"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01954"/>
        </source>
    </evidence>
    <sequence length="101" mass="10907" checksum="A25515EC093F7DA7" modified="2006-05-30" version="1">MIPGELMPLDGEIELNAGRPTVTVTVANTGDRPVQVGSHFHFYETNAALSFDREAARGYRLDIAAGTAVRFEPGQSRTVQLVALDGDRIVYGFNGRIMGAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LQ04</accession>
    <name>Q1LQ04_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Putative membrane protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Rmet_0886</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07772.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515712.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0886"/>
    <dbReference type="EnsemblBacteria" id="ABF07772">
        <property type="protein sequence ID" value="ABF07772"/>
        <property type="gene ID" value="Rmet_0886"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0886"/>
    <dbReference type="eggNOG" id="COG3748">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_058049_0_0_4"/>
    <dbReference type="OMA" id="YFNTRHD"/>
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        <property type="term" value="C:integral component of membrane"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="F:heme binding"/>
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        <property type="entry name" value="Urate_ox_N"/>
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    <keyword evidence="3 4" id="KW-0408">Iron</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="2 4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
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            <end position="33"/>
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            <end position="111"/>
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        </location>
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            <end position="172"/>
        </location>
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            <end position="197"/>
        </location>
    </feature>
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            <begin position="229"/>
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        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
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            <begin position="254"/>
            <end position="273"/>
        </location>
    </feature>
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        <location>
            <begin position="280"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="domain" description="Cytochrome c" evidence="6">
        <location>
            <begin position="312"/>
            <end position="398"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS51007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABF07772.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="402" mass="44324" checksum="16503E6048EA3FA2" modified="2006-05-30" version="1">MEGYILDWANLLLRWLHVITAIAWIGSSFYFVWLDNSLTRPTASDLKDKGVDGELWAVHGGGFYNPQKYLTAPKSLPENLHWFYWESYSTWMSGFALLVVLYLFNASTFLIDKNVYDMSPGAAVGFAVSYLVLGWVVYDSICRIFGKSDRTVGILVAVYVAVAAFVACHIFSGRAAFLLTGAMIATIMSANVLFWIIPGQRKVVASLKAGQPVDPIHGKRGKQRSVHNTYFTLPVLFAMLSNHYSMTYAAKNNWLVLIMVMLAGVLIRQFFILKHKGKINFAWPTAGVACLGVVAFMIAPQPRPQVASNAAGDAANAVSFVKVQEIINTRCVQCHAEQPKMMPTAAKGIKLDSADGIKAHAQLIYQQAVQQKAMPLGNVTQITDDERALLGQWFEGGAKTTN</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LQ37</accession>
    <name>Q1LQ37_CUPMC</name>
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        <submittedName>
            <fullName evidence="2">Serine/threonine protein phosphatase</fullName>
            <ecNumber evidence="2">3.1.3.16</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_0853</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
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        <property type="gene ID" value="Rmet_0853"/>
    </dbReference>
    <dbReference type="GeneID" id="60825337"/>
    <dbReference type="KEGG" id="rme:Rmet_0853"/>
    <dbReference type="eggNOG" id="COG0631">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_034545_5_0_4"/>
    <dbReference type="OMA" id="NNEDFHR"/>
    <dbReference type="OrthoDB" id="1835483at2"/>
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        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017018">
        <property type="term" value="F:myosin phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006470">
        <property type="term" value="P:protein dephosphorylation"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00143">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PP2C"/>
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    </dbReference>
    <dbReference type="InterPro" id="IPR001932">
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="2"/>
            <end position="243"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
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            <dbReference type="PROSITE" id="PS51746"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="ABF07739.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="304" mass="33750" checksum="1197BE13D6C33F57" modified="2006-05-30" version="1">MRFSVYQESRKGGRRINQDRMGYCFTRDALLMVLADGLGGHALGEVAAQQALQTLARQFQTQARPAIRNPADFLQDTIMLAHREIHRYAEANRLADIPRTTVVCCLIQHGQIHWAHAGDSRFYLMRKGALLTRTRDHSKIENLLQQERVLPMDVANHPERNKLYNCLGSPNLPLIDIGGPVRLNPGDVALLCSDGLWGSLEEDIIVDKVTHLSVVHAIPDLIERALANAGEGADNTTAIAMMWEADANVPNDDAVLTDTLPLNAFTTSILERTGSETDLLSEEEIERSIAEIRAAIDKTSNLMR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LQ84</accession>
    <name>Q1LQ84_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Putative MFS transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Rmet_0806</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07692.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0806"/>
    <dbReference type="EnsemblBacteria" id="ABF07692">
        <property type="protein sequence ID" value="ABF07692"/>
        <property type="gene ID" value="Rmet_0806"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0806"/>
    <dbReference type="eggNOG" id="COG2814">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001265_7_2_4"/>
    <dbReference type="OMA" id="FVWPIFG"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010645">
        <property type="entry name" value="MFS_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06779">
        <property type="entry name" value="MFS_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="SSF103473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="30"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="71"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="102"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="156"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="186"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="241"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="273"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="325"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="355"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="385"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="domain" description="MFS" evidence="6">
        <location>
            <begin position="235"/>
            <end position="423"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABF07692.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="423" mass="44302" checksum="CBFEA18A0E6769FB" modified="2006-05-30" version="1">MRRQPGFRHMPSANADISSGLLTRQTAGPWAVALAGLVSLALAMGIGRFAFTPLLPMMLHDGTVSLAEGSALATLNYIGYLLGALLCLFIRPDPVRMVRGALVLTVVLTLAMALPGGMVAWLAWRTLAGIASAVVMVYATAWCMQRLAELDHPTLGGLMFCGPGVGIVITGVSAFGMVAGHWLANWGWMTFAVLGAVMLALVWRVFGSPPPALHTPCGGSAPTLAPAPTPKLDLETWALTLAYGLAGFGYIITATFLPVIARQAMPGSVWADLFWPILGAGVMVGAYLVTRVGMHHDNRRMMMMLYTMQAIGVGVAAVWPTVAGFALSSLLVGLPFTALVSFAMREARRLWGQRASKLIGLMTAAYALGQIAGPPLATALVARTGGFSASLAVAAGALLLGALIYVWMWRALPVGGKPAHAGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LQG0</accession>
    <name>Q1LQG0_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Pyrimidine transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">rutG</name>
        <name evidence="9" type="ordered locus">Rmet_0730</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the nucleobase:cation symporter-2 (NCS2) (TC 2.A.40) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07616.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515577.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0730"/>
    <dbReference type="EnsemblBacteria" id="ABF07616">
        <property type="protein sequence ID" value="ABF07616"/>
        <property type="gene ID" value="Rmet_0730"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0730"/>
    <dbReference type="eggNOG" id="COG2233">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017959_1_1_4"/>
    <dbReference type="OMA" id="FGLCPKF"/>
    <dbReference type="OrthoDB" id="1115239at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015205">
        <property type="term" value="F:nucleobase transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006043">
        <property type="entry name" value="NCS2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006042">
        <property type="entry name" value="Xan_ur_permease"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00860">
        <property type="entry name" value="Xan_ur_permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00801">
        <property type="entry name" value="ncs2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01116">
        <property type="entry name" value="XANTH_URACIL_PERMASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="78"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="105"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="128"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="154"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="186"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="219"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="244"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="284"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="326"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="364"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="389"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="448"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ABF07616.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="482" mass="49167" checksum="08DC19BADD9864B6" modified="2006-05-30" version="1">MRNATADSGRRAVQSDKTIEQDQPTGVPGVNRAAAEKGENNMANGQGNAGGEGFFPKWRLKTEGVIGPDERLPAGQTLLAGIQHVVAMFGSTAIAPILMGFHPNIAILFSGIGTLLFFLFVRGRVPSYLGSSFAFIAVVIAATGYAGSGPNPNIPLALGGIIAAGALYTVIGLIVQAVGYGWVEKLMPPVVTGAIVAAIGLNLAPVAVKAVSAAPLDTWVGLLTVLAVGLIAVRAPGMLGRLPVLLGGLAGYLAYYIATNLMGLGKAIDFSGVAAANWFGMPEFTAPVFQANAMLLIAPVAIVLVAENLGHIKAIGVMTGRNLDPYIGRAFIGDGVATMLSAAGGGTGVTTYAENMGVMAVTKIYSTLIFVVAAAVAILLGFSPKFGALILTIPGPVIGGLTIVVFGLISATAGRIWVQNHVDFSSSRNLITVGATLTVAAGDLTLKLGGLTLGGIGTATFGAILLYHLLGKGRTQEQGLTH</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LQI9</accession>
    <name>Q1LQI9_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Glutamate and aspartate transporter subunit periplasmic-binding component of ABC superfamily</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">gltI</name>
        <name evidence="4" type="ordered locus">Rmet_0701</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07587.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515551.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0701"/>
    <dbReference type="EnsemblBacteria" id="ABF07587">
        <property type="protein sequence ID" value="ABF07587"/>
        <property type="gene ID" value="Rmet_0701"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0701"/>
    <dbReference type="eggNOG" id="COG0834">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019602_0_0_4"/>
    <dbReference type="OMA" id="QTETYAC"/>
    <dbReference type="OrthoDB" id="685252at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001638">
        <property type="entry name" value="Solute-binding_3/MltF_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00497">
        <property type="entry name" value="SBP_bac_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00062">
        <property type="entry name" value="PBPb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193345" evidence="2">
        <location>
            <begin position="30"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="domain" description="PBPb" evidence="3">
        <location>
            <begin position="43"/>
            <end position="273"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00062"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF07587.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="302" mass="33153" checksum="6AEEAE9D38DA3AD2" modified="2006-05-30" version="1">MKSLSVRPTPVTRLLAAAALLCAAAGAHAADGDTLKKIKDSGVISLGYRESSIPFSYSDGKEVMGYSHDYLLAIVDKVKSTLNMPNLQVKLTPITSQNRIPLMQNGTIDIECGSTTNNLERQKQVAFSNSLFIYGIRMLTKKDSGVTDFPQLKDKNVVTTAGTTGERLLVKMNGEGMNMNLTSAKDHGQAFLILESGRAAAFVMDEPLLYGERTKAKNAAEWVVAGKPLQTENYACMFRKDDPSFKKLVDGVVADIMTSGRGEKLYAKWFQSPIPPRNINMNYPLSPDMKQLFAEPNDKAFQ</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-10-31" modified="2022-08-03" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LQS4</accession>
    <name>CH60_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Chaperonin GroEL</fullName>
            <ecNumber evidence="1">5.6.1.7</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">60 kDa chaperonin</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Chaperonin-60</fullName>
            <shortName evidence="1">Cpn60</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">groEL</name>
        <name evidence="1" type="synonym">groL</name>
        <name type="ordered locus">Rmet_0616</name>
    </gene>
    <organism>
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Together with its co-chaperonin GroES, plays an essential role in assisting protein folding. The GroEL-GroES system forms a nano-cage that allows encapsulation of the non-native substrate proteins and provides a physical environment optimized to promote and accelerate protein folding.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + H2O + a folded polypeptide = ADP + phosphate + an unfolded polypeptide.</text>
            <dbReference type="EC" id="5.6.1.7"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Forms a cylinder of 14 subunits composed of two heptameric rings stacked back-to-back. Interacts with the co-chaperonin GroES.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the chaperonin (HSP60) family.</text>
    </comment>
    <dbReference type="EC" id="5.6.1.7" evidence="1"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07502.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008644493.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q1LQS4"/>
    <dbReference type="SMR" id="Q1LQS4"/>
    <dbReference type="STRING" id="266264.Rmet_0616"/>
    <dbReference type="EnsemblBacteria" id="ABF07502">
        <property type="protein sequence ID" value="ABF07502"/>
        <property type="gene ID" value="Rmet_0616"/>
    </dbReference>
    <dbReference type="GeneID" id="60824213"/>
    <dbReference type="KEGG" id="rme:Rmet_0616"/>
    <dbReference type="eggNOG" id="COG0459">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016503_3_0_4"/>
    <dbReference type="OMA" id="TDTDKME"/>
    <dbReference type="OrthoDB" id="265347at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016853">
        <property type="term" value="F:isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042026">
        <property type="term" value="P:protein refolding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd03344">
        <property type="entry name" value="GroEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.560.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.260.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.7.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00600">
        <property type="entry name" value="CH60"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018370">
        <property type="entry name" value="Chaperonin_Cpn60_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001844">
        <property type="entry name" value="Cpn60/GroEL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002423">
        <property type="entry name" value="Cpn60/GroEL/TCP-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027409">
        <property type="entry name" value="GroEL-like_apical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027413">
        <property type="entry name" value="GROEL-like_equatorial_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027410">
        <property type="entry name" value="TCP-1-like_intermed_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00118">
        <property type="entry name" value="Cpn60_TCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00298">
        <property type="entry name" value="CHAPERONIN60"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48592">
        <property type="entry name" value="SSF48592"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52029">
        <property type="entry name" value="SSF52029"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54849">
        <property type="entry name" value="SSF54849"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02348">
        <property type="entry name" value="GroEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00296">
        <property type="entry name" value="CHAPERONINS_CPN60"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0143">Chaperone</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0413">Isomerase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000256957" description="Chaperonin GroEL">
        <location>
            <begin position="1"/>
            <end position="547"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="30"/>
            <end position="33"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="51"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="87"/>
            <end position="91"/>
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        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="415"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="479"/>
            <end position="481"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="495"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00600"/>
        </source>
    </evidence>
    <sequence length="547" mass="57378" checksum="F080CCBA9C2A7EC6" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LQZ3</accession>
    <name>Q1LQZ3_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative membrane protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_0547</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07433.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515409.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0547"/>
    <dbReference type="EnsemblBacteria" id="ABF07433">
        <property type="protein sequence ID" value="ABF07433"/>
        <property type="gene ID" value="Rmet_0547"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0547"/>
    <dbReference type="eggNOG" id="COG2610">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_042638_1_1_4"/>
    <dbReference type="OMA" id="TAIWAVQ"/>
    <dbReference type="OrthoDB" id="1585405at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015128">
        <property type="term" value="F:gluconate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003474">
        <property type="entry name" value="Glcn_transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30354">
        <property type="entry name" value="PTHR30354"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="75"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="100"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="177"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="232"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="275"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="439"/>
            <end position="460"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF07433.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="463" mass="48614" checksum="4E7C723546230B31" modified="2006-05-30" version="1">MSFVIVLAALAFLMFAAYRGYSVILFAPIAALGAVLLTDPSAVAPVFTGIFMEKMVGFVKLYFPVFMLGAVFGKVIELSGFSESIVAAAIRYIGRSRANAVIVAVCALLTYGGVSLFVVVFAVYPFAAELYRQSNIPKRLMPGAIALGAFSFTMDSLPGTPQIQNIIPTTFFKTTSWAAPVLGVAGSLFIIIVGLSYLEWRRRVAAANGEGYGTNLLNEPQRVEGGKLPPPLLAILPLIVVGVSNFWLTRMIPLWYGEANSVSLPGLAKPVETKIASVTAIWAVEAALLLGIVVVLVTAFGAVRSRFAEGTKSAVSGALLASMNTASEYGFGGVIAALPGFLVVSDALRSIPNPLVNAAVSVSTLAGITGSASGGMSIALAAMSDVFIQGAHAAQIPLEVLHRVVSMASGGMDTLPHNGAVITLLAVTGLTHRQSYRDIFAITMIKTMAVFFVIFVFYATGLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="114" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LRS9</accession>
    <name>Q1LRS9_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Cytochrome c oxidase subunit 2</fullName>
            <ecNumber evidence="18">7.1.1.9</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="25" type="primary">coxB</name>
        <name evidence="25" type="ordered locus">Rmet_0261</name>
    </gene>
    <organism evidence="25 26">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="26" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="26">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="15 18">Subunits I and II form the functional core of the enzyme complex. Electrons originating in cytochrome c are transferred via heme a and Cu(A) to the binuclear center formed by heme a3 and Cu(B).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>4 Fe(II)-[cytochrome c] + 8 H(+)(in) + O2 = 4 Fe(III)-[cytochrome c] + 4 H(+)(out) + 2 H2O</text>
            <dbReference type="Rhea" id="RHEA:11436"/>
            <dbReference type="Rhea" id="RHEA-COMP:10350"/>
            <dbReference type="Rhea" id="RHEA-COMP:14399"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
            <dbReference type="EC" id="7.1.1.9"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="18">
            <name>Cu cation</name>
            <dbReference type="ChEBI" id="CHEBI:23378"/>
        </cofactor>
        <text evidence="18">Binds a copper A center.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="17">Cell membrane</location>
            <topology evidence="17">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Periplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 17">Belongs to the cytochrome c oxidase subunit 2 family.</text>
    </comment>
    <dbReference type="EC" id="7.1.1.9" evidence="18"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07147.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008642668.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0261"/>
    <dbReference type="EnsemblBacteria" id="ABF07147">
        <property type="protein sequence ID" value="ABF07147"/>
        <property type="gene ID" value="Rmet_0261"/>
    </dbReference>
    <dbReference type="GeneID" id="60822986"/>
    <dbReference type="KEGG" id="rme:Rmet_0261"/>
    <dbReference type="eggNOG" id="COG1622">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG2010">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036876_2_2_4"/>
    <dbReference type="OMA" id="HAFMPIA"/>
    <dbReference type="OrthoDB" id="1654242at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042597">
        <property type="term" value="C:periplasmic space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070469">
        <property type="term" value="C:respirasome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004129">
        <property type="term" value="F:cytochrome-c oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd13912">
        <property type="entry name" value="CcO_II_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.760.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.420">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045187">
        <property type="entry name" value="CcO_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002429">
        <property type="entry name" value="CcO_II-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034210">
        <property type="entry name" value="CcO_II_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001505">
        <property type="entry name" value="Copper_CuA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008972">
        <property type="entry name" value="Cupredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009056">
        <property type="entry name" value="Cyt_c-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036909">
        <property type="entry name" value="Cyt_c-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014222">
        <property type="entry name" value="Cyt_c_oxidase_su2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011759">
        <property type="entry name" value="Cyt_c_oxidase_su2_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036257">
        <property type="entry name" value="Cyt_c_oxidase_su2_TM_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22888">
        <property type="entry name" value="PTHR22888"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00116">
        <property type="entry name" value="COX2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02790">
        <property type="entry name" value="COX2_TM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13442">
        <property type="entry name" value="Cytochrome_CBB3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46626">
        <property type="entry name" value="SSF46626"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49503">
        <property type="entry name" value="SSF49503"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81464">
        <property type="entry name" value="SSF81464"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02866">
        <property type="entry name" value="CoxB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00078">
        <property type="entry name" value="COX2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50857">
        <property type="entry name" value="COX2_CUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50999">
        <property type="entry name" value="COX2_TM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51007">
        <property type="entry name" value="CYTC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13 18" id="KW-0186">Copper</keyword>
    <keyword evidence="10 17" id="KW-0249">Electron transport</keyword>
    <keyword evidence="5 16" id="KW-0349">Heme</keyword>
    <keyword evidence="12 16" id="KW-0408">Iron</keyword>
    <keyword evidence="14 20" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 16 18" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="25" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="26" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 17" id="KW-0679">Respiratory chain</keyword>
    <keyword evidence="21" id="KW-0732">Signal</keyword>
    <keyword evidence="9" id="KW-1278">Translocase</keyword>
    <keyword evidence="7 17 20" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 20" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4 17" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="21">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193734" description="Cytochrome c oxidase subunit 2" evidence="21">
        <location>
            <begin position="26"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="53"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="99"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="domain" description="COX2_TM" evidence="23">
        <location>
            <begin position="32"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="domain" description="COX2_CUA" evidence="22">
        <location>
            <begin position="128"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="domain" description="Cytochrome c" evidence="24">
        <location>
            <begin position="288"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="379"/>
            <end position="422"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007866"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00024688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU000456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="RuleBase" id="RU004024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS50999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="PROSITE" id="PS51007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="EMBL" id="ABF07147.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="422" mass="45654" checksum="2F0CB158F2DC7418" modified="2006-05-30" version="1">MKMWKKASAACLAGASLLVSQAASAVSDMPGGPAVRQLNLAEPVTKIAEQIHWLNWMMLIICTVIFVAVFGVMFYSIFKHRKSKGARPASFHESITVEVVWTIVPFLIVIAMALPATKTVVAMKDTTNSDITIKATGYQWKWGYDYLKGQGEGISFVSTLTTPREQINNQQEKSNTYLMEVDNELVVPVNKKIRIVTTANDVIHAWMIPAFGVKQDAIPGFVRDTWFRAEKTGVYRGQCAELCGKEHAFMPIVVRVVSDAEYTKWVDDKKKAMAAAADDPNKVWTLDEIKARGEKVYATNCAVCHQPTGKGGGPFPALDGSKVVNGPKAGQMHVLLEGRNAMPSWKQLSDTELAAVMTYTRNSWGNKTGEVIQPADFTGARTGKFPEGGGAQAGAGDAAPKAAAPSSTPDKQASLAGNRVAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LRX5</accession>
    <name>Q1LRX5_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Leucine/isoleucine/valine transporter subunit, ATP-binding component of ABC superfamily</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">livG8</name>
        <name evidence="10" type="ordered locus">Rmet_0215</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07101.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515120.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0215"/>
    <dbReference type="EnsemblBacteria" id="ABF07101">
        <property type="protein sequence ID" value="ABF07101"/>
        <property type="gene ID" value="Rmet_0215"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0215"/>
    <dbReference type="eggNOG" id="COG0411">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_2_4"/>
    <dbReference type="OMA" id="QHIRKTP"/>
    <dbReference type="OrthoDB" id="1179151at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032823">
        <property type="entry name" value="BCA_ABC_TP_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12399">
        <property type="entry name" value="BCA_ABC_TP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 7 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="1" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-1278">Translocase</keyword>
    <feature type="domain" description="ABC transporter" evidence="9">
        <location>
            <begin position="63"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="19"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="41"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="95"/>
            <end position="102"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ABF07101.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="313" mass="34376" checksum="BF3EE4C1B27DD671" modified="2006-05-30" version="1">MTVTQTAWQGGMARVDATGTLVPPGAIAPAGHVEGTHGDSPRPGSAREPESTSHVRTRGDVILDLQNISLSFGGVKALTDISFDVCEHEIRAIIGPNGAGKSSMLNVINGVYHPQQGRIVFRGQERRQMHPTAAARQGIARTFQNIALFKGMTVLDNIMTGRNTQFRTGVFAHALWWGPARAEEMRHRRKVEEVIDFLEIQAIRKTPVGRLPYGLQKRVELARALAAEPSMLLLDEPMAGMNVEEKQDMCRFILDVNHQFGTTIVLIEHDMGVVMDISDRVVVLDYGKKIGDGTPDEVKSNPDVIRAYLGTAH</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LRZ2</accession>
    <name>Q1LRZ2_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Co/Zn/Cd efflux system component</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">dmeF</name>
        <name evidence="7" type="ordered locus">Rmet_0198</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07084.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0198"/>
    <dbReference type="TCDB" id="2.A.4.3.9">
        <property type="family name" value="the cation diffusion facilitator (cdf) family"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF07084">
        <property type="protein sequence ID" value="ABF07084"/>
        <property type="gene ID" value="Rmet_0198"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0198"/>
    <dbReference type="eggNOG" id="COG1230">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013430_1_0_4"/>
    <dbReference type="OMA" id="REMDHPV"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008324">
        <property type="term" value="F:cation transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1510.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002524">
        <property type="entry name" value="Cation_efflux"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027469">
        <property type="entry name" value="Cation_efflux_TMD_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01545">
        <property type="entry name" value="Cation_efflux"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161111">
        <property type="entry name" value="SSF161111"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01297">
        <property type="entry name" value="CDF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="55"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="86"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="119"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="152"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="228"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="259"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="183"/>
            <end position="218"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABF07084.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="382" mass="41510" checksum="E34D77E00E1E0B5F" modified="2006-05-30" version="1">MKALSTAHAATLRAYFKPDPTHPDCTAMHTQNLSAWTHSHVFDAGNQAAERGTRLVMWITLAMMIVEIAAGLVFNSMALLADGWHMSSHALAIGLSAFAYAAARRLSQDGRFSFGTWKIEVLAAFASAIFLLGVAGLMVFGSVERLFTPQPIHYQEAMAITAIGLIVNLACALILGGAHHGHDHGHGHDHGHQAHGHDHHDHGHGHGHGHGGHDDHGHRHHDINLRSAYLHVVADAATSVLAIVALAGGWWLGWSWLDPVMGLVGAVLVGRWAIGLMRQSGTVLLDREMDHPVVEEVREVLAQFSHGEDGTRVADLHVWRVGREKFACIASLVTHDASLTPQRVRHALSIHDELVHVSVEINQCDDGRDGHDGHAAHRGHRH</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-01-15" modified="2022-08-03" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LS25</accession>
    <name>THIG_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Thiazole synthase</fullName>
            <ecNumber evidence="1">2.8.1.10</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">thiG</name>
        <name type="ordered locus">Rmet_0165</name>
    </gene>
    <organism>
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the rearrangement of 1-deoxy-D-xylulose 5-phosphate (DXP) to produce the thiazole phosphate moiety of thiamine. Sulfur is provided by the thiocarboxylate moiety of the carrier protein ThiS. In vitro, sulfur can be provided by H(2)S.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>1-deoxy-D-xylulose 5-phosphate + 2-iminoacetate + [sulfur-carrier protein ThiS]-C-terminal Gly-NH-CH2-C(O)SH = 2-[(2R,5Z)-2-carboxy-4-methylthiazol-5(2H)-ylidene]ethyl phosphate + [sulfur-carrier protein ThiS]-C-terminal Gly-Gly + 2 H(+) + 2 H2O</text>
            <dbReference type="Rhea" id="RHEA:26297"/>
            <dbReference type="Rhea" id="RHEA-COMP:12908"/>
            <dbReference type="Rhea" id="RHEA-COMP:12909"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57792"/>
            <dbReference type="ChEBI" id="CHEBI:62899"/>
            <dbReference type="ChEBI" id="CHEBI:77846"/>
            <dbReference type="ChEBI" id="CHEBI:90619"/>
            <dbReference type="ChEBI" id="CHEBI:90778"/>
            <dbReference type="EC" id="2.8.1.10"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; thiamine diphosphate biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homotetramer. Forms heterodimers with either ThiH or ThiS.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the ThiG family.</text>
    </comment>
    <dbReference type="EC" id="2.8.1.10" evidence="1"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07051.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008648498.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q1LS25"/>
    <dbReference type="SMR" id="Q1LS25"/>
    <dbReference type="STRING" id="266264.Rmet_0165"/>
    <dbReference type="EnsemblBacteria" id="ABF07051">
        <property type="protein sequence ID" value="ABF07051"/>
        <property type="gene ID" value="Rmet_0165"/>
    </dbReference>
    <dbReference type="GeneID" id="60822888"/>
    <dbReference type="KEGG" id="rme:Rmet_0165"/>
    <dbReference type="eggNOG" id="COG2022">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_062233_1_0_4"/>
    <dbReference type="OMA" id="PHNFQLI"/>
    <dbReference type="OrthoDB" id="784095at2"/>
    <dbReference type="UniPathway" id="UPA00060"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990107">
        <property type="term" value="F:thiazole synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009229">
        <property type="term" value="P:thiamine diphosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd04728">
        <property type="entry name" value="ThiG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00443">
        <property type="entry name" value="ThiG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033983">
        <property type="entry name" value="Thiazole_synthase_ThiG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008867">
        <property type="entry name" value="ThiG"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34266">
        <property type="entry name" value="PTHR34266"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05690">
        <property type="entry name" value="ThiG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0704">Schiff base</keyword>
    <keyword id="KW-0784">Thiamine biosynthesis</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_1000026034" description="Thiazole synthase">
        <location>
            <begin position="1"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="active site" description="Schiff-base intermediate with DXP" evidence="1">
        <location>
            <position position="113"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="174"/>
        </location>
        <ligand>
            <name>1-deoxy-D-xylulose 5-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:57792"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="201"/>
            <end position="202"/>
        </location>
        <ligand>
            <name>1-deoxy-D-xylulose 5-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:57792"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="223"/>
            <end position="224"/>
        </location>
        <ligand>
            <name>1-deoxy-D-xylulose 5-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:57792"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00443"/>
        </source>
    </evidence>
    <sequence length="282" mass="30408" checksum="D6C1E1503681AF7A" modified="2006-05-30" version="1">MTFEPTETLRDPFVLYGESFASRLLLGTARYPSPATLQAAVEASRPAMITVALRRQTAVGSGDGQTLGGETFWQMLRTLGVPVLPNTAGCFTAQEVITTAMMAREVFETDWIKLELIGDDYTLQPDTLNMPAVAETLVKEGFKVLPYCTEDLVLCRRLLDVGCQALMPWAAPIGTGRGAVNPHAMRTLRDRLPDTPLIVDAGLGLPSHAAQVLEWGYDGVLLNTAVAQSAYPVNMARAFALAVEAGRTAYLAGPMPEREVAQASTPVVGMPFWHAEGAEDRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LSH7</accession>
    <name>Q1LSH7_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Ribonucleotide pyrophosphatase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">mazG</name>
        <name evidence="1" type="ordered locus">Rmet_0013</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF06899.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0013"/>
    <dbReference type="EnsemblBacteria" id="ABF06899">
        <property type="protein sequence ID" value="ABF06899"/>
        <property type="gene ID" value="Rmet_0013"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0013"/>
    <dbReference type="eggNOG" id="COG1694">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_110454_1_0_4"/>
    <dbReference type="OMA" id="KMDTNRQ"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047429">
        <property type="term" value="F:nucleoside-triphosphate diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009143">
        <property type="term" value="P:nucleoside triphosphate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11537">
        <property type="entry name" value="NTP-PPase_RS21-C6_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025984">
        <property type="entry name" value="DCTPP"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12643">
        <property type="entry name" value="MazG-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF029826">
        <property type="entry name" value="UCP029826_pph"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF06899.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="118" mass="13048" checksum="47D9BD578E396ED5" modified="2006-05-30" version="1">MPVSDIHIAFTMALIDISNLQQAAAAFGEARGWGKYHSPKNLAMALSVEVAELVEIFQWQTEEESRGIMSTDERAHVEQELADITIYLTQLVTALGVDLDAAVQAKMEMNARKYPAPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-04-26" modified="2022-05-25" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>Q58AL0</accession>
    <name>Q58AL0_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Glycosyltransferase, family 39</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">gtrM3</name>
        <name evidence="11" type="ordered locus">Rmet_5962</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="11 12">
        <name>pMOL30</name>
    </geneLocation>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="12">Plasmid pMOL30</plasmid>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP000354">
        <property type="protein sequence ID" value="ABF12821.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011229353.1">
        <property type="nucleotide sequence ID" value="NC_006466.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_145608.1">
        <property type="nucleotide sequence ID" value="NC_006466.1"/>
    </dbReference>
    <dbReference type="CAZy" id="GT83">
        <property type="family name" value="Glycosyltransferase Family 83"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF12821">
        <property type="protein sequence ID" value="ABF12821"/>
        <property type="gene ID" value="Rmet_5962"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5962"/>
    <dbReference type="HOGENOM" id="CLU_019200_0_0_4"/>
    <dbReference type="OMA" id="DFWRFFF"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid pMOL30"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040845">
        <property type="entry name" value="Arnt_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038731">
        <property type="entry name" value="RgtA/B/C-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18583">
        <property type="entry name" value="Arnt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13231">
        <property type="entry name" value="PMT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="3" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-0614">Plasmid</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="87"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="113"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="137"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="168"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="208"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="257"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="296"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="343"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="383"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="409"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="domain" description="PMT_2" evidence="9">
        <location>
            <begin position="64"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="domain" description="Arnt_C" evidence="10">
        <location>
            <begin position="446"/>
            <end position="520"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF13231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF18583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ABF12821.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="561" mass="61914" checksum="8DDBF904A93BF5D9" modified="2005-04-26" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2005-01-04" modified="2021-09-29" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>Q5NUV3</accession>
    <name>Q5NUV3_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Diguanylate phosphodiesterase (EAL domain) from Tn4378(Mercury transposon)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">urf-2</name>
        <name evidence="2" type="ordered locus">Rmet_6186</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2 3">
        <name>pMOL28</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid pMOL28</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000355">
        <property type="protein sequence ID" value="ABF13045.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_003132006.1">
        <property type="nucleotide sequence ID" value="NC_007972.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_161724.1">
        <property type="nucleotide sequence ID" value="NC_006525.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF13045">
        <property type="protein sequence ID" value="ABF13045"/>
        <property type="gene ID" value="Rmet_6186"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6186"/>
    <dbReference type="HOGENOM" id="CLU_000445_70_50_4"/>
    <dbReference type="OMA" id="RIAGHAF"/>
    <dbReference type="OrthoDB" id="1635706at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid pMOL28"/>
    </dbReference>
    <dbReference type="CDD" id="cd01948">
        <property type="entry name" value="EAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.450">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001633">
        <property type="entry name" value="EAL_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035919">
        <property type="entry name" value="EAL_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021767">
        <property type="entry name" value="TnpM"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11809">
        <property type="entry name" value="DUF3330"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00563">
        <property type="entry name" value="EAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00052">
        <property type="entry name" value="EAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF141868">
        <property type="entry name" value="SSF141868"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50883">
        <property type="entry name" value="EAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="EAL" evidence="1">
        <location>
            <begin position="7"/>
            <end position="259"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF13045.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="329" mass="35688" checksum="CE09936CF1BA291C" modified="2005-01-04" version="1">MSAFRPDGWTTPELAQAVERGQLELHYQPVVDLRSGGIVGAEALLRWRHPTLGLLPPGQFLPVVESSGLMPEIGAWVLGEACRQMRDWRMLAWRPFRLAVNVSASQVGPDFDGWVKGVLADAELPAEYLEIELTESVAFGDPAIFPALDALRQIGVRFAADDFGTGYSCLQHLKCCPISTLKIDQSFVAGLANDRRDQTIVHTVIQLAHGLGMDVVAEGVETSASLDLLRQADCDTGQGFLFAKPMPAAAFAVFVSQWRGATMNASDSTTTSCCVCCKEIPLDAAFTPEGAEYVEHFCGLECYQRFEARAKTGNETDADPNACDSLPSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-01-04" modified="2022-05-25" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>Q5NV44</accession>
    <name>Q5NV44_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">VirB2 type IV secretion protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">trbC</name>
        <name evidence="3" type="ordered locus">Rmet_6303</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3 4">
        <name>pMOL28</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid pMOL28</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000355">
        <property type="protein sequence ID" value="ABF13162.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008649454.1">
        <property type="nucleotide sequence ID" value="NC_007972.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_161637.1">
        <property type="nucleotide sequence ID" value="NC_006525.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF13162">
        <property type="protein sequence ID" value="ABF13162"/>
        <property type="gene ID" value="Rmet_6303"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6303"/>
    <dbReference type="HOGENOM" id="CLU_2104939_0_0_4"/>
    <dbReference type="OMA" id="CAQSAMA"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid pMOL28"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007039">
        <property type="entry name" value="TrbC/VirB2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04956">
        <property type="entry name" value="TrbC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5013532130" evidence="2">
        <location>
            <begin position="36"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="59"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF13162.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="115" mass="11812" checksum="1CA51C8CE911049D" modified="2005-01-04" version="1">MTLTFNHRVRATARKYAPSALLMVGMMAVATSAVAADDAFLNNLGNFICGIATFINTKYLFVVGLIVIVLGAYAYANAESSLSKFISGACVGVGLAAAAPSILKSLGLIASCVGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-03-23" modified="2020-10-07" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>D3DXK2</accession>
    <name>D3DXK2_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_6404</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ADC45022.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADC45022">
        <property type="protein sequence ID" value="ADC45022"/>
        <property type="gene ID" value="Rmet_6404"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6404"/>
    <dbReference type="HOGENOM" id="CLU_2452426_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADC45022.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="89" mass="9467" checksum="54A0929800BCCB31" modified="2010-03-23" version="1">MVTAQPRVQYAPDFHAVPDSASPFCMAPTAIMRRQCCTAATADARNWSVPGPGAVLVDRENRPDCADGVTIGHPCPSEVERALRGICNA</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-03-23" modified="2020-10-07" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>D3DXN2</accession>
    <name>D3DXN2_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_6435</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ADC45052.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_6435"/>
    <dbReference type="EnsemblBacteria" id="ADC45052">
        <property type="protein sequence ID" value="ADC45052"/>
        <property type="gene ID" value="Rmet_6435"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6435"/>
    <dbReference type="HOGENOM" id="CLU_3347677_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADC45052.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="37" mass="4398" checksum="B227F2002616D46A" modified="2010-03-23" version="1">MPHNRHKLTTADPISAMSAKFYFYFFWHLTPLADREG</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-03-23" modified="2020-10-07" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>D3DY71</accession>
    <name>D3DY71_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 11">Heme exporter protein D</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">Rmet_6641</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="13">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="13">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 11">Required for the export of heme to the periplasm for the biogenesis of c-type cytochromes.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3 11">Cell inner membrane</location>
            <topology evidence="3 11">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 11">Belongs to the CcmD/CycX/HelD family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ADC45241.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADC45241">
        <property type="protein sequence ID" value="ADC45241"/>
        <property type="gene ID" value="Rmet_6641"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6641"/>
    <dbReference type="HOGENOM" id="CLU_3157000_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017004">
        <property type="term" value="P:cytochrome complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015886">
        <property type="term" value="P:heme transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007078">
        <property type="entry name" value="Haem_export_protD_CcmD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04995">
        <property type="entry name" value="CcmD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03141">
        <property type="entry name" value="cytochro_ccmD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="7 11" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11" id="KW-0201">Cytochrome c-type biogenesis</keyword>
    <keyword evidence="10 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-0614">Plasmid</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6 11" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="12"/>
            <end position="36"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002442"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004377"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00016461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU363101"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ADC45241.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="48" mass="5309" checksum="A4C1CCAC7548F9F6" modified="2010-03-23" version="1">MSTLNLDMLAGHAGYIASAFGMTFVLIAAELILLCARRRNSLRARSRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-03-23" modified="2020-10-07" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>D3DYC4</accession>
    <name>D3DYC4_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_6714</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ADC45294.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADC45294">
        <property type="protein sequence ID" value="ADC45294"/>
        <property type="gene ID" value="Rmet_6714"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6714"/>
    <dbReference type="HOGENOM" id="CLU_3047200_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADC45294.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="54" mass="6207" checksum="5552C2912FA2A1FA" modified="2010-03-23" version="1">MQVAIPGAQGTTHLKRRHYFIRLHFDKAHAKKARQIISTGRVPFFQRNGGINVH</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-03-23" modified="2020-10-07" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>D3DYC9</accession>
    <name>D3DYC9_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_6719</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ADC45299.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_6719"/>
    <dbReference type="EnsemblBacteria" id="ADC45299">
        <property type="protein sequence ID" value="ADC45299"/>
        <property type="gene ID" value="Rmet_6719"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6719"/>
    <dbReference type="HOGENOM" id="CLU_2882722_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADC45299.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="63" mass="7103" checksum="4C82200E8B52C927" modified="2010-03-23" version="1">MWTRAIGSQATEVAIRAEVLNRMVALARAPAIRSYRLSSASPRSPCFQSRFMQQRLTEATCMQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-04-07" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LA10</accession>
    <name>Q1LA10_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_6157</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="1 2">
        <name>pMOL30</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid pMOL30</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000354">
        <property type="protein sequence ID" value="ABF13016.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011514831.1">
        <property type="nucleotide sequence ID" value="NC_006466.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF13016">
        <property type="protein sequence ID" value="ABF13016"/>
        <property type="gene ID" value="Rmet_6157"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6157"/>
    <dbReference type="HOGENOM" id="CLU_1625716_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid pMOL30"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF13016.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="163" mass="18181" checksum="F9C90508ED43B62F" modified="2006-05-30" version="1">MNTIELQSIVFITLERVVFLTQEAWRHPNPSLQALVAPAQAWQCVSVDEMRPSLLLEVKHLDPSELPNWILSSSMEDVVDMLIHAPERIGAVQALVPSGLHKSGTWHLEPVVGIDEWVLGNRSGLECHTESGERYAFVPRPANSETHRQWKLPAAALCDGMTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LB74</accession>
    <name>Q1LB74_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">AMP-dependent synthetase and ligase, putative acetate-CoA ligase</fullName>
            <ecNumber evidence="4">6.2.1.1</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Rmet_5743</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="5">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="5">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EC" id="6.2.1.1" evidence="4"/>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12602.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011520144.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5743"/>
    <dbReference type="EnsemblBacteria" id="ABF12602">
        <property type="protein sequence ID" value="ABF12602"/>
        <property type="gene ID" value="Rmet_5743"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5743"/>
    <dbReference type="eggNOG" id="COG0365">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000022_59_10_4"/>
    <dbReference type="OMA" id="HAWSNLF"/>
    <dbReference type="OrthoDB" id="326708at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003987">
        <property type="term" value="F:acetate-CoA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025110">
        <property type="entry name" value="AMP-bd_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045851">
        <property type="entry name" value="AMP-bd_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020845">
        <property type="entry name" value="AMP-binding_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_Synth/Lig"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13193">
        <property type="entry name" value="AMP-binding_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00455">
        <property type="entry name" value="AMP_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0436">Ligase</keyword>
    <keyword evidence="4" id="KW-0614">Plasmid</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AMP-binding" evidence="2">
        <location>
            <begin position="52"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="domain" description="AMP-binding_C" evidence="3">
        <location>
            <begin position="457"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="543"/>
            <end position="567"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF13193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF12602.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="567" mass="63417" checksum="E2BABF8BAE556991" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="67" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LBB8</accession>
    <name>Q1LBB8_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_5699</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12558.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011520101.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5699"/>
    <dbReference type="EnsemblBacteria" id="ABF12558">
        <property type="protein sequence ID" value="ABF12558"/>
        <property type="gene ID" value="Rmet_5699"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5699"/>
    <dbReference type="eggNOG" id="COG1462">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_501437_0_0_4"/>
    <dbReference type="OMA" id="CCTIRID"/>
    <dbReference type="OrthoDB" id="286237at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030288">
        <property type="term" value="C:outer membrane-bounded periplasmic space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005534">
        <property type="entry name" value="Curli_assmbl/transp-comp_CsgG"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03783">
        <property type="entry name" value="CsgG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193033" evidence="2">
        <location>
            <begin position="23"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="27"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="412"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="29"/>
            <end position="44"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF12558.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="447" mass="46629" checksum="BABEDE365B2FE4DD" modified="2006-05-30" version="1">MISQARATLAATCAVALLVACGQQSPGDKPVAASPQNGAASNPTAQAGKPDVGVVERVHVSASGVGASAAEAVDQALRQAIMQVTGTTIDLSSTQFKTALGAAVGREAITLSSAGFAELVSQTTRGAVTNFKVVSLDAPGLMERVYKASIEADVAKFKAPADTGKLRIAIAPLRIGHVGGNNAERIAAELRQRITDALTQSGRFTVLERGDAPELYGEIERIASGEVSNDQFSKIGQGLGADLIWFGSVNAFETGRPTDADQTGRAGRWSVSQKFVNVTTREVLFSNTVDGGAAEQSGDRVDAMESAVVRKVVADILVRLYPVSIASRDGYNVVLSQGGQSVRQGATYQVVRLGKELTDPQTGRSLGFTEQECCTVVVDRVTASMSYGHLESVKINLDEVKSGELQLRAEAVSRNEPAGASSAAAKGGRKVVKKSAQLGPPTSDANW</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LBD0</accession>
    <name>Q1LBD0_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Type IV fimbrial biogenesis pilW-related transmembrane protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">pilW</name>
        <name evidence="2" type="ordered locus">Rmet_5687</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12546.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011520088.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5687"/>
    <dbReference type="EnsemblBacteria" id="ABF12546">
        <property type="protein sequence ID" value="ABF12546"/>
        <property type="gene ID" value="Rmet_5687"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5687"/>
    <dbReference type="eggNOG" id="COG4966">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_747788_0_0_4"/>
    <dbReference type="OMA" id="GDMTGTD"/>
    <dbReference type="OrthoDB" id="1697991at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043683">
        <property type="term" value="P:type IV pilus assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012902">
        <property type="entry name" value="N_methyl_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032092">
        <property type="entry name" value="PilW"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07963">
        <property type="entry name" value="N_methyl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16074">
        <property type="entry name" value="PilW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02532">
        <property type="entry name" value="IV_pilin_GFxxxE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00409">
        <property type="entry name" value="PROKAR_NTER_METHYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="21"/>
            <end position="43"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF12546.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="347" mass="36985" checksum="CB35ED2094EB94ED" modified="2006-05-30" version="1">MTRAHHAAVLHLRSRQRGFSLVELMVAITLTLIILTALSALYLNTSTTRNEFANSADQMENGRYVLDTMTRELELAGFFGPTNLSTKALVSSPDVCATDPSALGFSGSPVTMPLGVQGFAPGTIAPCLSNLLASSEILVVRRVSTTPVSAAVTGTPYLQASFCASDTSQMVFGASAAQFTLRTKTCDNTVPSALRQATVRIYYLAGCDQCGGSGDGVPTLKMAELSNGAFQIQSVAVGIQDMHLIYGVDLDSNGSPDCYVADPGSNNAAQCTTVTTYDWSVPLTNWKNVTAIRIGVLARTSRKTNGWTDTRTYDLGRAQAAGPFNDGYKRHIYAQTARLINVAGNRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="61" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LBF6</accession>
    <name>Q1LBF6_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_5661</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12520.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011520065.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5661"/>
    <dbReference type="EnsemblBacteria" id="ABF12520">
        <property type="protein sequence ID" value="ABF12520"/>
        <property type="gene ID" value="Rmet_5661"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5661"/>
    <dbReference type="HOGENOM" id="CLU_151799_0_0_4"/>
    <dbReference type="OMA" id="FREWNAF"/>
    <dbReference type="OrthoDB" id="1774931at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193295" evidence="1">
        <location>
            <begin position="34"/>
            <end position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF12520.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="121" mass="13396" checksum="BF317B44FE6A5125" modified="2006-05-30" version="1">MTRTFCTIHFARIRCAFRCLAVTGLLAFGNAHATDIDCDPSVVPARPVPAHRMICESALFSMGYQRIFADQQRQLKTGSITESEVAAFRKKRDACDSAACLDAVFREWKAFAAQAATLHKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="63" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LBG4</accession>
    <name>Q1LBG4_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_5653</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12512.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011520057.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5653"/>
    <dbReference type="EnsemblBacteria" id="ABF12512">
        <property type="protein sequence ID" value="ABF12512"/>
        <property type="gene ID" value="Rmet_5653"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5653"/>
    <dbReference type="eggNOG" id="COG3333">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022936_2_0_4"/>
    <dbReference type="OMA" id="LFFCCIG"/>
    <dbReference type="OrthoDB" id="735109at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002823">
        <property type="entry name" value="DUF112_TM"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01970">
        <property type="entry name" value="TctA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="26"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="63"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="111"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="140"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="171"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="208"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="324"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="357"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="393"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="419"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="467"/>
            <end position="492"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF12512.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="513" mass="54557" checksum="4FD76E92E96927C7" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LBL5</accession>
    <name>Q1LBL5_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">L-threonine dehydratase</fullName>
            <ecNumber evidence="3">4.3.1.19</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">Threonine deaminase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="3" type="primary">ilvA</name>
        <name evidence="5" type="ordered locus">Rmet_5602</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="6">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="6">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Catalyzes the anaerobic formation of alpha-ketobutyrate and ammonia from threonine in a two-step reaction. The first step involved a dehydration of threonine and a production of enamine intermediates (aminocrotonate), which tautomerizes to its imine form (iminobutyrate). Both intermediates are unstable and short-lived. The second step is the nonenzymatic hydrolysis of the enamine/imine intermediates to form 2-ketobutyrate and free ammonia. In the low water environment of the cell, the second step is accelerated by RidA.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>L-threonine = 2-oxobutanoate + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:22108"/>
            <dbReference type="ChEBI" id="CHEBI:16763"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:57926"/>
            <dbReference type="EC" id="4.3.1.19"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 3">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Amino-acid biosynthesis; L-isoleucine biosynthesis; 2-oxobutanoate from L-threonine: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homotetramer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the serine/threonine dehydratase family.</text>
    </comment>
    <dbReference type="EC" id="4.3.1.19" evidence="3"/>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12461.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5602"/>
    <dbReference type="EnsemblBacteria" id="ABF12461">
        <property type="protein sequence ID" value="ABF12461"/>
        <property type="gene ID" value="Rmet_5602"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5602"/>
    <dbReference type="eggNOG" id="COG1171">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021152_4_2_4"/>
    <dbReference type="OMA" id="PLQLNDH"/>
    <dbReference type="UniPathway" id="UPA00047">
        <property type="reaction ID" value="UER00054"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004794">
        <property type="term" value="F:L-threonine ammonia-lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009097">
        <property type="term" value="P:isoleucine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1100">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001926">
        <property type="entry name" value="PLP-dep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005787">
        <property type="entry name" value="Thr_deHydtase_biosynth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036052">
        <property type="entry name" value="Trypto_synt_PLP_dependent"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00291">
        <property type="entry name" value="PALP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53686">
        <property type="entry name" value="SSF53686"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01124">
        <property type="entry name" value="ilvA_2Cterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="3" id="KW-0100">Branched-chain amino acid biosynthesis</keyword>
    <keyword evidence="3" id="KW-0412">Isoleucine biosynthesis</keyword>
    <keyword evidence="3 5" id="KW-0456">Lyase</keyword>
    <keyword evidence="5" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2 3" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PALP" evidence="4">
        <location>
            <begin position="49"/>
            <end position="328"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU362012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABF12461.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="363" mass="38119" checksum="4E97F4E132876058" modified="2006-05-30" version="1">MPLTHPLAVPQAVAPGQSFDAEPFEAAHDAWSDADDYLRRILTARVYDVARETALEAAPTLSAHLGHRVWLKREDHQPVFSFKVRGAYNKMASLPADALARGVITASAGNHAQGVALAAARLGVRAIIVVPQSAPQVKVDAVRAHGSGMVEVVLAGESYSDAFDHALSLQQQHGLTFIPAFDDPDVIAGQGTVGMEILRQHRGPLDAVFVPIGGGSLAAGVAAYVKAVRPEVQVIGVQTEDSCAMARSLAAGERVTLDTVGLFSDGTAVKRVGEETFRLCQKYLDGVLTVDTDALCAAIKDIFQDTRSLVEPAGALAVAGLKQYAATAGNASLALVAITSGANMNFDRLRIVAERAEVVGAAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-10-07" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LBQ3</accession>
    <name>Q1LBQ3_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_5564</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12423.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF12423">
        <property type="protein sequence ID" value="ABF12423"/>
        <property type="gene ID" value="Rmet_5564"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5564"/>
    <dbReference type="eggNOG" id="ENOG503475D">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1509403_0_0_4"/>
    <dbReference type="OMA" id="IMATSSM"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF12423.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="178" mass="19758" checksum="B6D05D7A4D82672A" modified="2006-05-30" version="1">MPGNMPVLTCAIPKKKRHLLGSTYEKSNLGFGCIDLDEGRKLQTLRFQMGDLQFYFVADILDSTMWAAIDMWRTVGRLPFLFYVETDNSWDASFVVVDAITGPLRNEAFRGGPDAVPSASTAYELRDLVLSGQLQKAATSDIPGVPLRHVFVNIMATSSMAQALMPHVEVGRKARRYA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LBV5</accession>
    <name>Q1LBV5_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Enoyl-CoA hydratase/isomerase</fullName>
            <ecNumber evidence="4">4.2.1.17</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Rmet_5512</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="5">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="5">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Lipid metabolism; fatty acid beta-oxidation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the enoyl-CoA hydratase/isomerase family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.17" evidence="4"/>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12371.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519914.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5512"/>
    <dbReference type="EnsemblBacteria" id="ABF12371">
        <property type="protein sequence ID" value="ABF12371"/>
        <property type="gene ID" value="Rmet_5512"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5512"/>
    <dbReference type="eggNOG" id="COG1024">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009834_7_0_4"/>
    <dbReference type="OMA" id="CDVRYTT"/>
    <dbReference type="OrthoDB" id="1275789at2"/>
    <dbReference type="UniPathway" id="UPA00659"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004300">
        <property type="term" value="F:enoyl-CoA hydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016853">
        <property type="term" value="F:isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006635">
        <property type="term" value="P:fatty acid beta-oxidation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.12.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029045">
        <property type="entry name" value="ClpP/crotonase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045002">
        <property type="entry name" value="Ech1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001753">
        <property type="entry name" value="Enoyl-CoA_hydra/iso"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014748">
        <property type="entry name" value="Enoyl-CoA_hydra_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43149">
        <property type="entry name" value="PTHR43149"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00378">
        <property type="entry name" value="ECH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52096">
        <property type="entry name" value="SSF52096"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0413">Isomerase</keyword>
    <keyword evidence="4" id="KW-0456">Lyase</keyword>
    <keyword evidence="4" id="KW-0614">Plasmid</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF12371.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="286" mass="31293" checksum="20F349606C7C5967" modified="2006-05-30" version="1">MTWDSFTLSVEDHVAHLVLNRPEKRNALSRAFWTELPQAVQALDDGAQARVILISSTGPYFSSGIDIAMLGSLRPDMKGPDAERRARLIGPLTRYEGIVAFQRSFSSLEQCRLPVIAAVQGGCIGAGLDLLSACCIRYATSDAFFSVYEINVGMPADMGTLPRLTRFLPEGVVRELAYTGRRMDAREAHFRGLVNEVFDDHNSLLDKAKSVAREIASKAPIAIAGTKRLINYGRDHSTADTLDHVALWIAGMNPSEQILDAARANAGRASANFADLPVRTQFQKSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-10-07" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LBX3</accession>
    <name>Q1LBX3_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Extra-cytoplasmic solute receptor protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">bug</name>
        <name evidence="4" type="ordered locus">Rmet_5494</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="5">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="5">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Periplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the UPF0065 (bug) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12353.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5494"/>
    <dbReference type="EnsemblBacteria" id="ABF12353">
        <property type="protein sequence ID" value="ABF12353"/>
        <property type="gene ID" value="Rmet_5494"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5494"/>
    <dbReference type="eggNOG" id="COG3181">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045683_0_0_4"/>
    <dbReference type="OMA" id="YPEINIP"/>
    <dbReference type="OrthoDB" id="447734at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042597">
        <property type="term" value="C:periplasmic space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005064">
        <property type="entry name" value="BUG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042100">
        <property type="entry name" value="Bug_dom1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42928">
        <property type="entry name" value="PTHR42928"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03401">
        <property type="entry name" value="TctC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017082">
        <property type="entry name" value="YflP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-0675">Receptor</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004192991" evidence="3">
        <location>
            <begin position="30"/>
            <end position="329"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF12353.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="329" mass="34944" checksum="7A503C7ED89D07E9" modified="2006-05-30" version="1">MTTLPKLTRRALTAGISALLLSVSINALAQPEAFPTRPIRMIVPTAAGGNIDILARILAEHMSKAWGQQVVVDSKPGANSMLATTAVARSPADGYTVLMTLSGFVQNLVLQPNPPYKVNDLVPVSQVAWFPVALAANAKLPATDVASVVKLAKAQPDKLSFGSYGVGSGGHIIGEGLNNLAGIRIKHVGYKGESPAFTDLVGGNIELAYGSVGFYAHQLSGGKVRLIAVASPNRLKTFPNVPTFAEAGYPEINIPGWGGVFLPAKTPPAIVEKYTQEIRRIVALPEVQKKILEMGFEPTGTTSAQFSDLISSDIKRWGKIVRDNNIKLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="99" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LCA2</accession>
    <name>Q1LCA2_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative transcriptional regulator, LysR family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_5365</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the LysR transcriptional regulatory family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12224.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519770.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5365"/>
    <dbReference type="EnsemblBacteria" id="ABF12224">
        <property type="protein sequence ID" value="ABF12224"/>
        <property type="gene ID" value="Rmet_5365"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5365"/>
    <dbReference type="eggNOG" id="COG0583">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039613_16_0_4"/>
    <dbReference type="OMA" id="HSCLHYG"/>
    <dbReference type="OrthoDB" id="750230at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005119">
        <property type="entry name" value="LysR_subst-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000847">
        <property type="entry name" value="Tscrpt_reg_HTH_LysR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00126">
        <property type="entry name" value="HTH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03466">
        <property type="entry name" value="LysR_substrate"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50931">
        <property type="entry name" value="HTH_LYSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH lysR-type" evidence="2">
        <location>
            <begin position="3"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF12224.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="300" mass="33147" checksum="E5C26BACAC2B10A0" modified="2006-05-30" version="1">MKTTIDELLAFAAVVDSGSITAAAEQLGLTISATSRTLSRLEEKLKTTLLRRTTRRLELTEEGAAFLEHARAILAMVDEAEEQMAARQMRPVGRLRVDAATPFMLHVIVPLVAGFRASYPEVELELNSNEGFIDLIEKRTDVAFRIGQLKDSTLHARPIGISQIRVLASPEYLKRHGTPTKPAQLAQHALLGFTQPESLNDWPLRDDAGGVVHIKPTIASSSGETLRSLALAGQGIVCLSDFMTLEDRQSGRLVQLFAKQTLDVRQPINAVYYRNTALASRITCFLDHVVRTLGKHPFER</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LCH9</accession>
    <name>Q1LCH9_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Outer membrane porin protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Rmet_5288</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="8">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="8">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12147.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519692.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5288"/>
    <dbReference type="EnsemblBacteria" id="ABF12147">
        <property type="protein sequence ID" value="ABF12147"/>
        <property type="gene ID" value="Rmet_5288"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5288"/>
    <dbReference type="eggNOG" id="COG3203">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038238_2_0_4"/>
    <dbReference type="OMA" id="HWNQGNL"/>
    <dbReference type="OrthoDB" id="1064531at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046930">
        <property type="term" value="C:pore complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015288">
        <property type="term" value="F:porin activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006811">
        <property type="term" value="P:ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00342">
        <property type="entry name" value="gram_neg_porins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.160.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033900">
        <property type="entry name" value="Gram_neg_porin_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023614">
        <property type="entry name" value="Porin_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002299">
        <property type="entry name" value="Porin_Neis"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13609">
        <property type="entry name" value="Porin_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00184">
        <property type="entry name" value="NEISSPPORIN"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0406">Ion transport</keyword>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-0626">Porin</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1134">Transmembrane beta strand</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193060" evidence="5">
        <location>
            <begin position="20"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="domain" description="Porin_4" evidence="6">
        <location>
            <begin position="7"/>
            <end position="345"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF13609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABF12147.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="386" mass="41052" checksum="8867AB323889290B" modified="2006-05-30" version="1">MNKRLFALAAACACGAANAQSSVTLYGVVDVNVEYVNHVGAVPQAGNGYNPGPPQTVFRENSGGYSGSRWGLRGVEDLGGGLKSIFVLENGFNADTGTLQQGGRMFGRQAFVGLDTRFGVFTFGRQYQSIFSTMANFVPARYATQYEPTGVVAGANFRQDNTVKYSGTFGPFTAQADWSFGVGTTLPSTYPTVPTAGGAGEVPGQFRRDSAYGVALSYLAGPVGLGIGYDQWNPTIGTGNGTFKKAVVMASYAFNDTAKLMGGYRWGQNKDPGGTTILRDDFYWIGGQYRFSSALDFTLEYDYQNVKNLGGNNGVANPWQIALIADYAFSKRTDVYLTTAYSRNAGLAMESAASGFATSLALGNSYALANGQKSMFGAALGIRHVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="95" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LCL5</accession>
    <name>Q1LCL5_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Flagellin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">fliC2</name>
        <name evidence="6" type="ordered locus">Rmet_5252</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="7">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="7">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Flagellin is the subunit protein which polymerizes to form the filaments of bacterial flagella.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Secreted</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Bacterial flagellum</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the bacterial flagellin family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12111.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519657.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5252"/>
    <dbReference type="EnsemblBacteria" id="ABF12111">
        <property type="protein sequence ID" value="ABF12111"/>
        <property type="gene ID" value="Rmet_5252"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5252"/>
    <dbReference type="eggNOG" id="COG1344">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011142_7_2_4"/>
    <dbReference type="OMA" id="IASQTTY"/>
    <dbReference type="OrthoDB" id="636786at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009288">
        <property type="term" value="C:bacterial-type flagellum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005198">
        <property type="term" value="F:structural molecule activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001492">
        <property type="entry name" value="Flagellin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046358">
        <property type="entry name" value="Flagellin_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042187">
        <property type="entry name" value="Flagellin_C_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001029">
        <property type="entry name" value="Flagellin_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42792">
        <property type="entry name" value="PTHR42792"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00700">
        <property type="entry name" value="Flagellin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00669">
        <property type="entry name" value="Flagellin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00207">
        <property type="entry name" value="FLAGELLIN"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0975">Bacterial flagellum</keyword>
    <keyword evidence="6" id="KW-0966">Cell projection</keyword>
    <keyword evidence="6" id="KW-0969">Cilium</keyword>
    <keyword evidence="6" id="KW-0282">Flagellum</keyword>
    <keyword evidence="6" id="KW-0614">Plasmid</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <feature type="domain" description="Flagellin_N" evidence="4">
        <location>
            <begin position="5"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="domain" description="Flagellin_C" evidence="5">
        <location>
            <begin position="313"/>
            <end position="397"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005709"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023143"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU362073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00669"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00700"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABF12111.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="398" mass="40936" checksum="367381566F62DA91" modified="2006-05-30" version="1">MAQVINTNSLSLMTQNNLNTSQSALNTAIQRLSSGLRINSAKDDAAGLAIASRFTSNINGLTVAQRNANDGISLAQTTEGALTEVTNNLQRIRDLSVQAANGSNSASDLKSIQDEITQRMSEIDRLSAQTDFNGVKVLSSTAKSVNIQVGAQDGQTIAVNLQEISTKTLGLNSFNVNGPQGAVTAATAANFQKVFGSSTTFTTSSITEAVGGTLATTLGISSSSVAIGNQAMVDANGNWFAQVTVTPASATEAQALKNQGMTTATSGSAMTMYVALNPNSTTSTTGATASFTLNTTGVTASTLTGSASANPLATLDNALQTVNSLRSSLGAIQNRLDSTISNLGTTITNLSSSRSRIQDADYATEVSNMTRANILQQAGTSVLAQANQTTQGVLSLLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LCR9</accession>
    <name>Q1LCR9_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">D-3-phosphoglycerate dehydrogenase, NAD-binding</fullName>
            <ecNumber evidence="4">1.1.1.95</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Rmet_5198</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="5">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="5">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.95" evidence="4"/>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12057.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519604.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5198"/>
    <dbReference type="EnsemblBacteria" id="ABF12057">
        <property type="protein sequence ID" value="ABF12057"/>
        <property type="gene ID" value="Rmet_5198"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5198"/>
    <dbReference type="eggNOG" id="COG1052">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019796_1_2_4"/>
    <dbReference type="OMA" id="DDIKWNH"/>
    <dbReference type="OrthoDB" id="1638924at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004617">
        <property type="term" value="F:phosphoglycerate dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006139">
        <property type="entry name" value="D-isomer_2_OHA_DH_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006140">
        <property type="entry name" value="D-isomer_DH_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00389">
        <property type="entry name" value="2-Hacid_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02826">
        <property type="entry name" value="2-Hacid_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-0614">Plasmid</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="2-Hacid_dh" evidence="2">
        <location>
            <begin position="47"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="domain" description="2-Hacid_dh_C" evidence="3">
        <location>
            <begin position="111"/>
            <end position="282"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU003719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF02826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF12057.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="317" mass="33832" checksum="F58487EE368832AA" modified="2006-05-30" version="1">MSLACMKDEGEVIVLTRLPLDQDWLRAHAPRYRFVPWTAVDEAQRVQARAVLTNGSTGLTGAEMAALPALELVASFGAGYENIERDAARMRGVRVCHAPDTNSQVVADHALAMMLAWSRGIAMLDRGLKAGQWDALRAPRPGVRGKTLGIVGLGNIGQRLAALAEAVGMRVAYLRRSGALQPNRTPYGDVRSLAAASDVLALTCPGGPATRHLVDAEVLRALGPDGLLVNVARGSVVDTEALVQALRRGELGGAALDVLEAEPLVPEAFREMDQVILTPHMAGRSPETRVAQHEALLRNLDGWFRHAAPAHPVDLAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LCV9</accession>
    <name>Q1LCV9_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Acyl-CoA ligase putative o-succinylbenzoate-CoA ligase</fullName>
            <ecNumber evidence="4">6.2.1.26</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Rmet_5158</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="5">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="5">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EC" id="6.2.1.26" evidence="4"/>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12017.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519563.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5158"/>
    <dbReference type="EnsemblBacteria" id="ABF12017">
        <property type="protein sequence ID" value="ABF12017"/>
        <property type="gene ID" value="Rmet_5158"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5158"/>
    <dbReference type="eggNOG" id="COG0318">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000022_59_0_4"/>
    <dbReference type="OMA" id="ILIEYYA"/>
    <dbReference type="OrthoDB" id="377638at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008756">
        <property type="term" value="F:o-succinylbenzoate-CoA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025110">
        <property type="entry name" value="AMP-bd_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045851">
        <property type="entry name" value="AMP-bd_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020845">
        <property type="entry name" value="AMP-binding_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_Synth/Lig"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13193">
        <property type="entry name" value="AMP-binding_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00455">
        <property type="entry name" value="AMP_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0436">Ligase</keyword>
    <keyword evidence="4" id="KW-0614">Plasmid</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AMP-binding" evidence="2">
        <location>
            <begin position="6"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="domain" description="AMP-binding_C" evidence="3">
        <location>
            <begin position="424"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="507"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="507"/>
            <end position="521"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF13193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF12017.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="527" mass="57558" checksum="550286FD3671D2F8" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LCX0</accession>
    <name>Q1LCX0_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Extra-cytoplasmic solute receptor (Bug)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">bugT</name>
        <name evidence="4" type="ordered locus">Rmet_5147</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="5">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="5">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Periplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the UPF0065 (bug) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12006.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519553.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5147"/>
    <dbReference type="EnsemblBacteria" id="ABF12006">
        <property type="protein sequence ID" value="ABF12006"/>
        <property type="gene ID" value="Rmet_5147"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5147"/>
    <dbReference type="eggNOG" id="COG3181">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045683_0_0_4"/>
    <dbReference type="OMA" id="WFGVMAR"/>
    <dbReference type="OrthoDB" id="447734at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042597">
        <property type="term" value="C:periplasmic space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005064">
        <property type="entry name" value="BUG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042100">
        <property type="entry name" value="Bug_dom1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42928">
        <property type="entry name" value="PTHR42928"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03401">
        <property type="entry name" value="TctC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017082">
        <property type="entry name" value="YflP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-0675">Receptor</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193322" evidence="3">
        <location>
            <begin position="24"/>
            <end position="320"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF12006.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="320" mass="33173" checksum="A10C03C254746095" modified="2006-05-30" version="1">MLHSAKMLFLAVAVFAVSNAASAADQPIRLVVPYPPGGAADQITRLVTSDASKFLKTTIVIDNKPGAAGIIAAETVARSAPDGKTLLVGSNAPLVINSAIYEKLPYDPARDFVPVAGLGKTPLMLSVRAELPVKSVKDLVALGKGQPGKLTMGSASSGNITHLAGENASSKLGVKVTHVPFAGSAPAIVSLIGGNIDLIYDALPSSMQQARANRIHALAILDTNRFPLLPDVPTMHELGYSGTEASAWFGVVAPAGTPAAPIAAMNKAINDALRNPELVEKLRAIGAQPMPGSPASFGEFINEERARWIPVAKSLNVKAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LCZ2</accession>
    <name>Q1LCZ2_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">APH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_5125</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11984.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519531.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5125"/>
    <dbReference type="EnsemblBacteria" id="ABF11984">
        <property type="protein sequence ID" value="ABF11984"/>
        <property type="gene ID" value="Rmet_5125"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5125"/>
    <dbReference type="eggNOG" id="COG0645">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG2187">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026771_1_1_4"/>
    <dbReference type="OMA" id="FRFVDVM"/>
    <dbReference type="OrthoDB" id="402265at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002575">
        <property type="entry name" value="Aminoglycoside_PTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01636">
        <property type="entry name" value="APH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="SSF56112"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="APH" evidence="1">
        <location>
            <begin position="57"/>
            <end position="280"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01636"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF11984.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="497" mass="55228" checksum="D1BA95CB03765954" modified="2006-05-30" version="1">MDDAALTYALLQPSAYPPPVQDVQVIETHISRVFLAGQFAYKVRKPVKFDFVDFSTPQARHADCEAELRLNRRFAPALYLDVLPIVAGETPGAVVVGGRGTPLEYAVRMRRFAQHNLLIDMIGAGRLGPDHIDALARDIAIFHGAQPRAAPHEGFGTPASIDATLEECLDGLARLTIDRNMTDRVANAARARVAALQDTFQARLRHGHVRECHGDLHLGNIVLLDGNPTPFDCLEFEPRLRWIDTASDIAFPFMDLLHHGRQDLAYRLLNAYLERHGDYAGLAVLPFYVALRALVRARVLLERARQRHTESTALDLVHVECRTLLNLAFQFLTLPAAPLCVMHGLSGSGKSTVAATMSEARAMVRVRSDVERKRLRNANAQQESRYSDSVTARTYHRLLAICRLGCAAGFPMIADATFLSHDQRARFANQARRLGVRFFIVDCKAPVETLRARIVARALARQDPSDADLAVLDLQLRTHDPLTTEERIHVVPADHPG</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="72" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LD14</accession>
    <name>Q1LD14_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative deaminase-reductase (RibD domain)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_5103</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11962.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519508.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5103"/>
    <dbReference type="EnsemblBacteria" id="ABF11962">
        <property type="protein sequence ID" value="ABF11962"/>
        <property type="gene ID" value="Rmet_5103"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5103"/>
    <dbReference type="eggNOG" id="COG0262">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_043966_4_2_4"/>
    <dbReference type="OMA" id="GYIAKPN"/>
    <dbReference type="OrthoDB" id="1306955at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008703">
        <property type="term" value="F:5-amino-6-(5-phosphoribosylamino)uracil reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009231">
        <property type="term" value="P:riboflavin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.430.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024072">
        <property type="entry name" value="DHFR-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002734">
        <property type="entry name" value="RibDG_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01872">
        <property type="entry name" value="RibD_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53597">
        <property type="entry name" value="SSF53597"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RibD_C" evidence="1">
        <location>
            <begin position="6"/>
            <end position="170"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01872"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF11962.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="177" mass="19128" checksum="854E981F667E6814" modified="2006-05-30" version="1">MTTAHVFIATSLDGFIARPDGDIGWLLQRDDPAEDHGYMDFIADKDAIVMGRGTYEKVLTLGDWAYDRPVLVLSRQLAGTPVPAALEGKVRFASLSPRDTLERLAAEGVRRVYVDGGQVVQSFLRDGLIADLVVTTVPVLIGAGRPLFGALPRDVDLALVSSRSFPSGLVQSTYRVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LD62</accession>
    <name>Q1LD62_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative epoxide hydrolase</fullName>
            <ecNumber evidence="3">3.3.2.9</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_5052</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EC" id="3.3.2.9" evidence="3"/>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11914.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519464.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5052"/>
    <dbReference type="ESTHER" id="ralme-q1ld62">
        <property type="family name" value="Epoxide_hydrolase"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF11914">
        <property type="protein sequence ID" value="ABF11914"/>
        <property type="gene ID" value="Rmet_5052"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5052"/>
    <dbReference type="eggNOG" id="COG0596">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020336_16_1_4"/>
    <dbReference type="OMA" id="WPYDINA"/>
    <dbReference type="OrthoDB" id="1439833at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033961">
        <property type="term" value="F:cis-stilbene-oxide hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000639">
        <property type="entry name" value="Epox_hydrolase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006311">
        <property type="entry name" value="TAT_signal"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00561">
        <property type="entry name" value="Abhydrolase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00412">
        <property type="entry name" value="EPOXHYDRLASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="SSF53474"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51318">
        <property type="entry name" value="TAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AB hydrolase-1" evidence="2">
        <location>
            <begin position="83"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="31"/>
            <end position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF11914.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="352" mass="38175" checksum="5B9350244AABB321" modified="2006-05-30" version="1">MPEIVNLPRRGFMGVVAATLAAAQFGGAQTARAQTSQSKATPLPQPLPRPGTHTSFAPIKQIKAGVLNVGYAEAGPTNGPVALLLHGWPYDIYSFVDVAPLLAARGYRVIMPYLRGYGTTSFLSSDTMRNGQPSAIAADMIALLDALGIQNAVVAGFDWGARTADIMAALWPDRCRGLVSVSGYLIGSQAGGKVPLPPEAELQWWYQFYFATDRGRAGYQKYTHQFAKLIWKLASPKWDFDDATFERSAAAFDNPDHVDITVHNYRWRQGLATGEARFDDIEKRLATAPTISVPTITMEGDANGAPHPEPSAYASKFTGRYEHRNVTGGIGHNLPQEAPAAFAQAVLDVDRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="96" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LDS2</accession>
    <name>Q1LDS2_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Putative ABC transporter, inner membrane component</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Rmet_4842</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="8">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="8">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cell membrane</location>
            <topology evidence="4">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the binding-protein-dependent transport system permease family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11704.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519267.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4842"/>
    <dbReference type="EnsemblBacteria" id="ABF11704">
        <property type="protein sequence ID" value="ABF11704"/>
        <property type="gene ID" value="Rmet_4842"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4842"/>
    <dbReference type="eggNOG" id="COG1177">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016047_3_4_4"/>
    <dbReference type="OMA" id="FRRSWLF"/>
    <dbReference type="OrthoDB" id="1264541at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="SSF161098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0614">Plasmid</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2 4" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="12"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="64"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="103"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="132"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="179"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="238"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="6">
        <location>
            <begin position="64"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="268"/>
            <end position="291"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABF11704.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="291" mass="31702" checksum="23F62825FC592FF8" modified="2006-05-30" version="1">MTSKPRRTAYWLQLTLTLAVCAFMTVPVVLSVLAGLTNNIFVGLSSGLTTRWLVEVWSLYRNTIFLSLGIALACLACTLVIGVPAAYYMALRRNRVTRLIEELLMLPVAVPGLATALGLILLYGGWQALRTSWVFILIGHVLFTLPFMVRSVLAILSAIDIRTIEDAAASLGATRLQRFFTVVLPNCRQGILAGALMVVTLSVGEFNLTWMLHTPTTQTLPVGLADSYASMRLEIGSAYTIVFFVMIIPLLVFMQALSALGARARRHAPPPEATAELPMTAVPQSAETRHA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LDU2</accession>
    <name>Q1LDU2_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">YcaO domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_4822</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11684.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519247.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4822"/>
    <dbReference type="EnsemblBacteria" id="ABF11684">
        <property type="protein sequence ID" value="ABF11684"/>
        <property type="gene ID" value="Rmet_4822"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4822"/>
    <dbReference type="eggNOG" id="COG1765">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG1944">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022530_0_0_4"/>
    <dbReference type="OMA" id="FRRMFGN"/>
    <dbReference type="OrthoDB" id="1068793at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015946">
        <property type="entry name" value="KH_dom-like_a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003718">
        <property type="entry name" value="OsmC/Ohr_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036102">
        <property type="entry name" value="OsmC/Ohrsf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003776">
        <property type="entry name" value="YcaO-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041080">
        <property type="entry name" value="YcaO_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019938">
        <property type="entry name" value="YcaO_dom_prot"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02566">
        <property type="entry name" value="OsmC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02624">
        <property type="entry name" value="YcaO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18381">
        <property type="entry name" value="YcaO_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82784">
        <property type="entry name" value="SSF82784"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00702">
        <property type="entry name" value="TIGR00702"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03549">
        <property type="entry name" value="TIGR03549"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51664">
        <property type="entry name" value="YCAO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="YcaO" evidence="1">
        <location>
            <begin position="206"/>
            <end position="573"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF11684.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="734" mass="81639" checksum="C9F61199FDE450AB" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LE82</accession>
    <name>Q1LE82_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Thiosulphate:quinone oxidoreductase (TQO) small subunit(DoxD domain)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">doxX</name>
        <name evidence="7" type="ordered locus">Rmet_4682</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="8">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="8">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the DoxX family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11544.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4682"/>
    <dbReference type="EnsemblBacteria" id="ABF11544">
        <property type="protein sequence ID" value="ABF11544"/>
        <property type="gene ID" value="Rmet_4682"/>
    </dbReference>
    <dbReference type="GeneID" id="60821707"/>
    <dbReference type="KEGG" id="rme:Rmet_4682"/>
    <dbReference type="eggNOG" id="COG2259">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_058421_4_1_4"/>
    <dbReference type="OMA" id="PTHGTWA"/>
    <dbReference type="OrthoDB" id="2025630at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032808">
        <property type="entry name" value="DoxX"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07681">
        <property type="entry name" value="DoxX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0614">Plasmid</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="28"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="92"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="129"/>
            <end position="149"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABF11544.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="156" mass="17260" checksum="3AEC136E65D2B27B" modified="2006-05-30" version="1">MSNVSHTDSPVRADTTLWSNLTTRASRLISDSFLALLARLAIASVFFLSGRTKVTGLLTIKDSTYSLFEQEYNLPLIPPELAAHLAAYAEHLFPVLLVLGLFTRFSAFALLGMTLVIEIFVYPDAWPTHLTWAALQLFLIGRGAGAFSLDRRFGIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LER0</accession>
    <name>Q1LER0_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Extra-cytoplasmic solute receptor protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">bug</name>
        <name evidence="4" type="ordered locus">Rmet_4501</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="5">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="5">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Periplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the UPF0065 (bug) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11366.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518959.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4501"/>
    <dbReference type="EnsemblBacteria" id="ABF11366">
        <property type="protein sequence ID" value="ABF11366"/>
        <property type="gene ID" value="Rmet_4501"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4501"/>
    <dbReference type="eggNOG" id="COG3181">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045683_0_2_4"/>
    <dbReference type="OMA" id="FVVPNRV"/>
    <dbReference type="OrthoDB" id="447734at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042597">
        <property type="term" value="C:periplasmic space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005064">
        <property type="entry name" value="BUG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042100">
        <property type="entry name" value="Bug_dom1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006311">
        <property type="entry name" value="TAT_signal"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42928">
        <property type="entry name" value="PTHR42928"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03401">
        <property type="entry name" value="TctC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017082">
        <property type="entry name" value="YflP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51318">
        <property type="entry name" value="TAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-0675">Receptor</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193431" evidence="3">
        <location>
            <begin position="30"/>
            <end position="327"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF11366.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="327" mass="34481" checksum="92946150512D3DC7" modified="2006-05-30" version="1">MQTSRRTWLAQAGTLAGAAMLGGVGQALAQPSYPSKPIRLVVPYPAGGGTDTVGRMIGQRLAEAWGQPVVVDNKPGASGMLGNDTVAKAAPDGYTVLLAITALIQSPSLYKRMPYDVNKDFTPVSLIAKSSDLFVVPNRVPASTLREFIALAKAGKLSYGSYGNGTSSHLHGELLRQQTGVELTHIPYKGAAPLLNDLLGGQVDSAFVDVTSAYPYLTSGKFKILGITGTQRYKAIPNVPTFAEQGLPGFEPSGWFALFLPANAPKDVTSRMAAEVARIVKLPEISQKLAGMGLQPVGSAPQELAAVVSGDMPKWAKIVQDAHIQLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-10-07" version="60" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LEX4</accession>
    <name>Q1LEX4_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_4437</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11302.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4437"/>
    <dbReference type="EnsemblBacteria" id="ABF11302">
        <property type="protein sequence ID" value="ABF11302"/>
        <property type="gene ID" value="Rmet_4437"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4437"/>
    <dbReference type="eggNOG" id="COG1288">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045224_0_0_4"/>
    <dbReference type="OMA" id="GTMAGGN"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="51"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="106"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="180"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="236"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="260"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="291"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="331"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="367"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="413"/>
            <end position="436"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF11302.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="437" mass="44940" checksum="585E2ED203FA81CA" modified="2006-05-30" version="1">MPGETSLWGLTPVLVFAIIALLGVDIVLSTIAAIVLGAIMTGTTPVGMGKLLADSLGSFIAVVGLIIVLGAGVGEVASRTGAAEQLVRAIVRRIGLSNQVRVRLGIMVTSTLICGALGTLAGGNAIIAAVVIPIAAAVRLSPPAVAALFQTAGSAGLVLGPFTPNVVTVLGLTGLSYPEYLMVAGIPMAAATLASGWFMSGRIQKMTEAEHQYDEAKNGIAAFDLSATPPRTAVRAAWAFCLTIIGLATAGVIVKAGFAFAIIVMVSLAATTGLAGGMGPRAILQAMYAGAGKLIWIFFLFWLFNPVLVLVDKLNAYHALLDIASPHLAGIGGAMLCVIILWFNVIGHIPGAAVAQMTFTAKIFGPLLAAAGVGPAATTAVILGSSQIDWFGPFPTSDMFGQMGLARSTQLKYMLYNGWAVIIVNLCLFSGLFFLLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LF00</accession>
    <name>Q1LF00_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Acetyl CoA synthetase</fullName>
            <ecNumber evidence="3">6.2.1.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">pauA</name>
        <name evidence="3" type="ordered locus">Rmet_4411</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EC" id="6.2.1.-" evidence="3"/>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11276.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4411"/>
    <dbReference type="EnsemblBacteria" id="ABF11276">
        <property type="protein sequence ID" value="ABF11276"/>
        <property type="gene ID" value="Rmet_4411"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4411"/>
    <dbReference type="eggNOG" id="COG1042">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007415_3_1_4"/>
    <dbReference type="OMA" id="EDFEHMF"/>
    <dbReference type="OrthoDB" id="445933at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016874">
        <property type="term" value="F:ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.261">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011761">
        <property type="entry name" value="ATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013815">
        <property type="entry name" value="ATP_grasp_subdomain_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003781">
        <property type="entry name" value="CoA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032875">
        <property type="entry name" value="Succ_CoA_lig_flav_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016102">
        <property type="entry name" value="Succinyl-CoA_synth-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13380">
        <property type="entry name" value="CoA_binding_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13607">
        <property type="entry name" value="Succ_CoA_lig"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00881">
        <property type="entry name" value="CoA_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52210">
        <property type="entry name" value="SSF52210"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50975">
        <property type="entry name" value="ATP_GRASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0436">Ligase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ATP-grasp" evidence="2">
        <location>
            <begin position="492"/>
            <end position="528"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF11276.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="712" mass="73576" checksum="9675D9A1E01F00C8" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LFA4</accession>
    <name>Q1LFA4_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Major facilitator superfamily MFS_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Rmet_4306</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="8">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="8">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11172.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518797.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4306"/>
    <dbReference type="EnsemblBacteria" id="ABF11172">
        <property type="protein sequence ID" value="ABF11172"/>
        <property type="gene ID" value="Rmet_4306"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4306"/>
    <dbReference type="eggNOG" id="COG2814">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001265_7_1_4"/>
    <dbReference type="OMA" id="WEIFAAI"/>
    <dbReference type="OrthoDB" id="1345431at2"/>
    <dbReference type="Proteomes" id="UP000002429">
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
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        <property type="entry name" value="MFS_dom"/>
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    <proteinExistence type="predicted"/>
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    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="22"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="56"/>
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        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="92"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="117"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="148"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="181"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="237"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="268"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="296"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="319"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="360"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="386"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="domain" description="MFS" evidence="6">
        <location>
            <begin position="17"/>
            <end position="411"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABF11172.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="413" mass="42955" checksum="37ACBCE1EA5CA716" modified="2006-05-30" version="1">MSQNTVTTPVPDTTDRALDARALHAALAGLCASLVAIGLARFAYTPLIPSLIQAHWFSAADTVALGAANFAGYLAGAVIGRPLATRLSNRHALRLLMVLATAAFFACAVPVSVSWFFLWRFLSGLTGGAIMVLVATAILPHIPPGRRAFVSGMIFLGLGLGIAASGTLVPELLKLGLGQTWLGLGAVSLLLTAISWKGWPTADVPTGMPAPTAAGHRPVDHAQGSSNPALRVLYFQYAANALGLVPAMMLLVDYVARGLGQGASAGAGYWVLYGVAAIAGPLLAGAIGGKLGFRNAYRLAMLLQGLAVGLLAVSHHTVALWLSTIVLGTFTPGIVPLVLGRVQEMLPHDATAQRAAWSRATIAFALCQALGGYGYAYLFAQSHENYSLIFLCGAVAMAVAFVTDFAVRKPKTD</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="96" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LFA9</accession>
    <name>Q1LFA9_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">L-aspartate oxidase</fullName>
            <ecNumber evidence="4">1.4.3.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">Rmet_4301</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="13">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="13">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-aspartate + O2 = H2O2 + iminosuccinate</text>
            <dbReference type="Rhea" id="RHEA:25876"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
            <dbReference type="ChEBI" id="CHEBI:77875"/>
            <dbReference type="EC" id="1.4.3.16"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="9">
            <dbReference type="Rhea" id="RHEA:25877"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Cofactor biosynthesis; NAD(+) biosynthesis; iminoaspartate from L-aspartate (oxidase route): step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the FAD-dependent oxidoreductase 2 family. NadB subfamily.</text>
    </comment>
    <dbReference type="EC" id="1.4.3.16" evidence="4"/>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11167.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518794.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4301"/>
    <dbReference type="EnsemblBacteria" id="ABF11167">
        <property type="protein sequence ID" value="ABF11167"/>
        <property type="gene ID" value="Rmet_4301"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4301"/>
    <dbReference type="eggNOG" id="COG0029">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014312_8_4_4"/>
    <dbReference type="OMA" id="RLMGNAL"/>
    <dbReference type="OrthoDB" id="153138at2"/>
    <dbReference type="UniPathway" id="UPA00253">
        <property type="reaction ID" value="UER00326"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008734">
        <property type="term" value="F:L-aspartate oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044318">
        <property type="term" value="F:L-aspartate:fumarate oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="term" value="P:NAD biosynthetic process"/>
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        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003953">
        <property type="entry name" value="FAD-binding_2"/>
    </dbReference>
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        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005288">
        <property type="entry name" value="NadB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027477">
        <property type="entry name" value="Succ_DH/fumarate_Rdtase_cat_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42716">
        <property type="entry name" value="PTHR42716"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00890">
        <property type="entry name" value="FAD_binding_2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SSF56425"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8 12" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="12" id="KW-0614">Plasmid</keyword>
    <keyword evidence="6" id="KW-0662">Pyridine nucleotide biosynthesis</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD_binding_2" evidence="11">
        <location>
            <begin position="86"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004950"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008562"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00029281"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00890"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ABF11167.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="549" mass="57997" checksum="A05080221B2D17A0" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="58" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LFE6</accession>
    <name>Q1LFE6_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Hypothetical membrane associated protein putative exported protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_4264</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11130.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518756.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4264"/>
    <dbReference type="EnsemblBacteria" id="ABF11130">
        <property type="protein sequence ID" value="ABF11130"/>
        <property type="gene ID" value="Rmet_4264"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4264"/>
    <dbReference type="HOGENOM" id="CLU_2342065_0_0_4"/>
    <dbReference type="OrthoDB" id="1982535at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193487" evidence="2">
        <location>
            <begin position="21"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="16"/>
            <end position="37"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF11130.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="81" mass="8527" checksum="3996CF8BCE29DFF4" modified="2010-03-23" version="2">MKAIVLATTALLVATSSAHAAPRKPDPFTDGGRASATQAQIAVRNTSQPLVEEQFRTLAVRKADPYTDGAIRTVDVFTDGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="98" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LFF8</accession>
    <name>Q1LFF8_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Fimbrial biogenesis outer membrane usher protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">htrE</name>
        <name evidence="14" type="ordered locus">Rmet_4252</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="15">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="15">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 10">Cell outer membrane</location>
            <topology evidence="2 10">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the fimbrial export usher family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11118.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518744.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4252"/>
    <dbReference type="EnsemblBacteria" id="ABF11118">
        <property type="protein sequence ID" value="ABF11118"/>
        <property type="gene ID" value="Rmet_4252"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4252"/>
    <dbReference type="eggNOG" id="COG3188">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009120_3_1_4"/>
    <dbReference type="OMA" id="FYLSGRV"/>
    <dbReference type="OrthoDB" id="120032at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009279">
        <property type="term" value="C:cell outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015473">
        <property type="term" value="F:fimbrial usher porin activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009297">
        <property type="term" value="P:pilus assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.2070">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.2610">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.410">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000015">
        <property type="entry name" value="Fimb_usher"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018030">
        <property type="entry name" value="Fimbrial_membr_usher_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042186">
        <property type="entry name" value="FimD_plug_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025949">
        <property type="entry name" value="PapC-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043142">
        <property type="entry name" value="PapC-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025885">
        <property type="entry name" value="PapC_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037224">
        <property type="entry name" value="PapC_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30451">
        <property type="entry name" value="PTHR30451"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13953">
        <property type="entry name" value="PapC_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13954">
        <property type="entry name" value="PapC_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00577">
        <property type="entry name" value="Usher"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF141729">
        <property type="entry name" value="SSF141729"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01151">
        <property type="entry name" value="FIMBRIAL_USHER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 10" id="KW-0998">Cell outer membrane</keyword>
    <keyword evidence="10" id="KW-1029">Fimbrium biogenesis</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-0614">Plasmid</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 11" id="KW-0732">Signal</keyword>
    <keyword evidence="6 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1134">Transmembrane beta strand</keyword>
    <keyword evidence="4 10" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193093" evidence="11">
        <location>
            <begin position="30"/>
            <end position="850"/>
        </location>
    </feature>
    <feature type="domain" description="PapC_N" evidence="13">
        <location>
            <begin position="40"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="domain" description="PapC_C" evidence="12">
        <location>
            <begin position="767"/>
            <end position="832"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004571"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU003884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF13953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF13954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ABF11118.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="850" mass="90691" checksum="C4238B3E0F855FF3" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LFX4</accession>
    <name>Q1LFX4_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Alkaline phosphatase</fullName>
            <ecNumber evidence="3">3.1.3.1</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">phoA2</name>
        <name evidence="3" type="ordered locus">Rmet_4085</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the alkaline phosphatase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.1" evidence="3"/>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10952.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518579.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4085"/>
    <dbReference type="EnsemblBacteria" id="ABF10952">
        <property type="protein sequence ID" value="ABF10952"/>
        <property type="gene ID" value="Rmet_4085"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4085"/>
    <dbReference type="eggNOG" id="COG1785">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008539_4_1_4"/>
    <dbReference type="OMA" id="RIMSKGM"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004035">
        <property type="term" value="F:alkaline phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd16012">
        <property type="entry name" value="ALP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.720.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001952">
        <property type="entry name" value="Alkaline_phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017850">
        <property type="entry name" value="Alkaline_phosphatase_core_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11596">
        <property type="entry name" value="PTHR11596"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00245">
        <property type="entry name" value="Alk_phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00113">
        <property type="entry name" value="ALKPHPHTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00098">
        <property type="entry name" value="alkPPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53649">
        <property type="entry name" value="SSF53649"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193455" evidence="2">
        <location>
            <begin position="27"/>
            <end position="467"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU003946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF10952.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="467" mass="48616" checksum="C40F6C051F3798B9" modified="2006-05-30" version="1">MKITTRIGMLAAAAASACLTMPAAQAAGEAKNVIFFLGDGMGPATVTASRIYKYGESGKLTMESLKRTARIKTFSNDAQTTDSAPSMAAYMTGVKMNNEVISMSPDTKATAPGTDVNGNKTINNCLPTNGTSVPTLLELAKARGKATGAITTTELTHATPAATYSHICHRDAQYDIAAQAVPGGAGFNTALGDGVDVLMGGGRNHWTPFDATANKKGRADGRNLLTEFAAKGYAVAATRDQMMQASGNKLIGLFSTTSHLEYEIDRVAGKGEGATQPSLADMTAKAIELLSKNGNGYFLMVEGGRIDHALHGTNAKRALEDTIAFDDAIKRALSMVDLSNTIIVVTADHDHTMTINGYSHRGNPILATATDYKTKKTALAADGLPYTTLVFGNGGTPRKATRDNPSLVDTTANDYLQEVGVNLGSPGAETHGGGDVMLFSTGPGSAPLKGTLDNTKVFGVVKSALGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LFZ2</accession>
    <name>Q1LFZ2_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Methyltransf_11 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_4066</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10934.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4066"/>
    <dbReference type="EnsemblBacteria" id="ABF10934">
        <property type="protein sequence ID" value="ABF10934"/>
        <property type="gene ID" value="Rmet_4066"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4066"/>
    <dbReference type="eggNOG" id="COG0500">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_082414_0_0_4"/>
    <dbReference type="OMA" id="WHDRATF"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013216">
        <property type="entry name" value="Methyltransf_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08241">
        <property type="entry name" value="Methyltransf_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Methyltransf_11" evidence="1">
        <location>
            <begin position="129"/>
            <end position="227"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF08241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF10934.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="294" mass="31805" checksum="64EDA7B9885679ED" modified="2006-05-30" version="1">MYRLAGLCAGYAYLLKKPLSKVSRRPHAVVTPLRRRWNISRRDLIRDRGLREGAEDGKSVVLVPSTEIAVAAAGPTMPIARKAMQSKDHWETVYATKPPTEVSWYQSAPTLSLDLIRKLDLPADATIADIGGGASTLVDHLLSLGLHRLVVVDLAGHALAAARDRLGARAAGVRWIVGDVTTPVLPEASADLWHDRAVFHFLTASEDRAAYVAQARRAVRPGGHLIIATFALDGPTQCSGLPVVRYDVAGLRAEFAPAFDLVAHTTETHTTPAGHAQSFLYCHFVPHPSALMPD</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="116" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LGA2</accession>
    <name>Q1LGA2_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Cytochrome c oxidase subunit 1</fullName>
            <ecNumber evidence="8">7.1.1.9</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">Rmet_3956</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="12">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="12">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Cytochrome c oxidase is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Subunits 1-3 form the functional core of the enzyme complex. CO I is the catalytic subunit of the enzyme. Electrons originating in cytochrome c are transferred via the copper A center of subunit 2 and heme A of subunit 1 to the bimetallic center formed by heme A3 and copper B.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>4 Fe(II)-[cytochrome c] + 8 H(+)(in) + O2 = 4 Fe(III)-[cytochrome c] + 4 H(+)(out) + 2 H2O</text>
            <dbReference type="Rhea" id="RHEA:11436"/>
            <dbReference type="Rhea" id="RHEA-COMP:10350"/>
            <dbReference type="Rhea" id="RHEA-COMP:14399"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
            <dbReference type="EC" id="7.1.1.9"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 8">Energy metabolism; oxidative phosphorylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cell membrane</location>
            <topology evidence="8">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the heme-copper respiratory oxidase family.</text>
    </comment>
    <dbReference type="EC" id="7.1.1.9" evidence="8"/>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10824.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518463.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
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    <dbReference type="EnsemblBacteria" id="ABF10824">
        <property type="protein sequence ID" value="ABF10824"/>
        <property type="gene ID" value="Rmet_3956"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3956"/>
    <dbReference type="eggNOG" id="COG0843">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011899_7_3_4"/>
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    <dbReference type="OrthoDB" id="316745at2"/>
    <dbReference type="UniPathway" id="UPA00705"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
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        <property type="term" value="C:integral component of membrane"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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    </dbReference>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="project" value="UniProtKB-EC"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="term" value="P:electron transport coupled proton transport"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="P:oxidative phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Cyt_c_oxase-like_su1_dom"/>
    </dbReference>
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        <property type="entry name" value="Cyt_c_oxase-like_su1_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000883">
        <property type="entry name" value="Cyt_C_Oxase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023615">
        <property type="entry name" value="Cyt_c_Oxase_su1_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014241">
        <property type="entry name" value="Cyt_c_oxidase_su1_bac"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10422">
        <property type="entry name" value="PTHR10422"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00115">
        <property type="entry name" value="COX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01165">
        <property type="entry name" value="CYCOXIDASEI"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81442">
        <property type="entry name" value="SSF81442"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02891">
        <property type="entry name" value="CtaD_CoxA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50855">
        <property type="entry name" value="COX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00077">
        <property type="entry name" value="COX1_CUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-0186">Copper</keyword>
    <keyword evidence="7" id="KW-0249">Electron transport</keyword>
    <keyword evidence="7" id="KW-0349">Heme</keyword>
    <keyword evidence="8" id="KW-0408">Iron</keyword>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-0614">Plasmid</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0679">Respiratory chain</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="7" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="27"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="69"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="109"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="158"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="200"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="270"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="299"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="326"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="362"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="397"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="440"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="482"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="domain" description="COX1" evidence="10">
        <location>
            <begin position="15"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="563"/>
            <end position="585"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00029368"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU363061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50855"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ABF10824.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="585" mass="65082" checksum="D52129BC73E3B441" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="108" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LGB0</accession>
    <name>Q1LGB0_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Transcriptional regulator, Crp/Fnr family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_3948</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="EnsemblBacteria" id="ABF10816">
        <property type="protein sequence ID" value="ABF10816"/>
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    </dbReference>
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    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="project" value="InterPro"/>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018490">
        <property type="entry name" value="cNMP-bd-like"/>
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        <property type="entry name" value="cNMP-bd_dom"/>
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        <property type="entry name" value="HTH_CRP"/>
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        <property type="entry name" value="RmlC-like_jellyroll"/>
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        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="HTH_CRP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH crp-type" evidence="2">
        <location>
            <begin position="126"/>
            <end position="199"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS51063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF10816.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="219" mass="24098" checksum="37DABA79F0F5DC9C" modified="2006-05-30" version="1">MLPDRPALSPERKHLQAGEALFRQDQPVRAVYPLRHGGIKRMYESALGWRQLTGFMLPGDVAGLEPNDAPQFGTSAIALQASECCVVPLETAKRCEEDPAVHAALLALLHRDALRQRELLVTIGSMKAAQRLAMLLLDQAAEHRRRGTDGGEVMLAMTRNDIASYLGLTLETVSRLLSRFASVGLITVRHRQLRIVDEKGLSDVHAEADRITLRGPDPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="97" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LGF1</accession>
    <name>Q1LGF1_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_3907</name>
    </gene>
    <organism evidence="3 4">
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        <lineage>
            <taxon>Bacteria</taxon>
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        </lineage>
    </organism>
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        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10775.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518421.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3907"/>
    <dbReference type="EnsemblBacteria" id="ABF10775">
        <property type="protein sequence ID" value="ABF10775"/>
        <property type="gene ID" value="Rmet_3907"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3907"/>
    <dbReference type="eggNOG" id="COG2771">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_692075_0_0_4"/>
    <dbReference type="OMA" id="YANSWAE"/>
    <dbReference type="OrthoDB" id="1755994at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06170">
        <property type="entry name" value="LuxR_C_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016032">
        <property type="entry name" value="Sig_transdc_resp-reg_C-effctor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000792">
        <property type="entry name" value="Tscrpt_reg_LuxR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00196">
        <property type="entry name" value="GerE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00421">
        <property type="entry name" value="HTH_LUXR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46894">
        <property type="entry name" value="SSF46894"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50043">
        <property type="entry name" value="HTH_LUXR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH luxR-type" evidence="2">
        <location>
            <begin position="305"/>
            <end position="370"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF10775.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="392" mass="42193" checksum="A038578DA9AC14C6" modified="2006-05-30" version="1">MGEHPSTDDVFHRLVDCIYESAMDVAAMPAALDLFSRYTGTGSPSYLIWDKLAARARLGVTPHGCFTSAGSMPDNMRALPPHLTTTGATRACATMSVDGATAMLAEPEAAAATSIYDCSGLAQGIRLFDTSDVCVLMSATPASCDAFAHCQQRVAQVMPHWARAARMQHRNFELSGLASLGLASLDTLDFGVMVLQADMRVRYANSWAEALVQADTNLALDNGVLRARAESMQSVLAGLLESAVHGGRNGTVAGSWMHITSGGQPVPIIVMPLVSRQPVEGPWQLPMAMMLLGNSESRSVLDAGVLTSLFGLSRKESIIAIRLAAGETLTEIAEREFLSPHTVRVHIRDTLRKTGTHRQSELVRLLHLLPGVDLERAGVSSTARPRDTRRRH</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-10-07" version="72" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LGF8</accession>
    <name>Q1LGF8_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Extra-cytoplasmic solute receptor protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">bug</name>
        <name evidence="3" type="ordered locus">Rmet_3900</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Periplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the UPF0065 (bug) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10768.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3900"/>
    <dbReference type="EnsemblBacteria" id="ABF10768">
        <property type="protein sequence ID" value="ABF10768"/>
        <property type="gene ID" value="Rmet_3900"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3900"/>
    <dbReference type="eggNOG" id="COG3181">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045683_0_0_4"/>
    <dbReference type="OMA" id="HINANRV"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042597">
        <property type="term" value="C:periplasmic space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005064">
        <property type="entry name" value="BUG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042100">
        <property type="entry name" value="Bug_dom1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42928">
        <property type="entry name" value="PTHR42928"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03401">
        <property type="entry name" value="TctC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-0675">Receptor</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF10768.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="398" mass="42323" checksum="2BF09BE9DC8BD8D2" modified="2006-05-30" version="1">MTGGADWRGCGLSRDFFMPSPPLRQSRIVPPGQWIHAARYSNPRNQTRMTRAFRRQRMTTFPQLPAIGTGKWRGMTWAALALAAVAVAVPGQGRANDAYPSKPIVMVVPFAAGGPTDVVARSVAAAMSKTLGQSVVVENRLGAGGTVSAAYVAKAAPDGYTILIHHNGMATAPALYSKLPYKPLTDFSFVGQVADVPMTLLGRHDLPPNNLPELVTYIQKNQNKVNLANAGLGAVSQLCGMLFQKAIGVDVQTIPYQGTAPAMTALLGGQVDVLCDQTTQTLTHIKADKVKLYGVTTAERIPALPNAPTLREGGLKGFEVKVWHGIYAPKGTPPAVINKLNGALRAALKDPAVAARMQDLGAVIVPEDKQTPEGLRTWLASEIDKWSPIIKAAGVKAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LGM5</accession>
    <name>Q1LGM5_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Probable membrane transporter protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Rmet_3831</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="7">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="7">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cell membrane</location>
            <topology evidence="5">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the 4-toluene sulfonate uptake permease (TSUP) (TC 2.A.102) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10701.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF10701">
        <property type="protein sequence ID" value="ABF10701"/>
        <property type="gene ID" value="Rmet_3831"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3831"/>
    <dbReference type="eggNOG" id="COG0730">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045498_5_1_4"/>
    <dbReference type="OMA" id="MYHHIIN"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002781">
        <property type="entry name" value="TM_pro_TauE-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01925">
        <property type="entry name" value="TauE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0614">Plasmid</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="31"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="71"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="97"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="127"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="165"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="204"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="229"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="258"/>
            <end position="275"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU363041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABF10701.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="277" mass="28937" checksum="9A6862E54B644C8A" modified="2006-05-30" version="1">MSAYDRGLAFSEGRFSQPRPRFTMPDHSLLSALLIFGLGGLLGGVGGLFGIGGGLIAIPALGLLYGMDQQLAQGTSLVMIAPNVLIGFWQYRKRADIELKTAIVLGLSAVLATYASAKFATSIDAALLRRIFAVFMIGLALYFLWRLLPGRATTQPQARLSPSWIPAVGVVGGAFSGFFSVGGGVVAAPALVGLFGMRQAAAQGLALALVTPGAVVALFTYANAGHVDWYSGIPLSLGGMLTISWGVALAHRMPERKLRAAFALCLIATAVVMLVRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LGP5</accession>
    <name>Q1LGP5_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Extra-cytoplasmic solute receptor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">bugT</name>
        <name evidence="4" type="ordered locus">Rmet_3811</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="5">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="5">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Periplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the UPF0065 (bug) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10681.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518328.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3811"/>
    <dbReference type="EnsemblBacteria" id="ABF10681">
        <property type="protein sequence ID" value="ABF10681"/>
        <property type="gene ID" value="Rmet_3811"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3811"/>
    <dbReference type="eggNOG" id="COG3181">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045683_0_0_4"/>
    <dbReference type="OMA" id="YEAYTWN"/>
    <dbReference type="OrthoDB" id="447734at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042597">
        <property type="term" value="C:periplasmic space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005064">
        <property type="entry name" value="BUG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042100">
        <property type="entry name" value="Bug_dom1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42928">
        <property type="entry name" value="PTHR42928"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03401">
        <property type="entry name" value="TctC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017082">
        <property type="entry name" value="YflP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-0675">Receptor</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193160" evidence="3">
        <location>
            <begin position="28"/>
            <end position="327"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF10681.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="327" mass="34099" checksum="EAB1F0F6AB14D70E" modified="2006-05-30" version="1">MIPSLYRLGAALICAGALLATATPAAADAGYPAKPIRLVVPYAAGGPTDTFARALVAGWSRQLKAQIVIENRTGAGTLVGTEYVSKATPDGYTLLLTTVAHAVNPSIHDALPYRTIEDFAPVGLAARAPLVLVVNKKLPVGNLKEFIAYLKAHPGQVNYSSAGVGSAPHLGAELLNYMSDTKTVHVPYRGSAPAMADLIGGHVAFMFDSAPTGLAQVRAGTVKLLATTMAARLPQTPDAPAVAEVVPGYEAYTWNAVFAPANTPPEVIRKLTDTLGATLKEPALQAKAVDLGLQLEPNPTPQALAQFVRAEIAKWKPVAVAANMKAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LGQ9</accession>
    <name>Q1LGQ9_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Extra-cytoplasmic solute receptor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">bugT</name>
        <name evidence="4" type="ordered locus">Rmet_3797</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="5">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="5">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Periplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the UPF0065 (bug) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10667.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518316.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3797"/>
    <dbReference type="EnsemblBacteria" id="ABF10667">
        <property type="protein sequence ID" value="ABF10667"/>
        <property type="gene ID" value="Rmet_3797"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3797"/>
    <dbReference type="eggNOG" id="COG3181">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045683_0_0_4"/>
    <dbReference type="OMA" id="NTESRYQ"/>
    <dbReference type="OrthoDB" id="447734at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042597">
        <property type="term" value="C:periplasmic space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005064">
        <property type="entry name" value="BUG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042100">
        <property type="entry name" value="Bug_dom1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42928">
        <property type="entry name" value="PTHR42928"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03401">
        <property type="entry name" value="TctC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017082">
        <property type="entry name" value="YflP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-0675">Receptor</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193444" evidence="3">
        <location>
            <begin position="21"/>
            <end position="320"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF10667.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="320" mass="34035" checksum="2B5C41B9285602EE" modified="2006-05-30" version="1">MLRIFLAAVMLVLANGSALADAYPSRPVRLLVGYAPGGPVDTAARIYADYLGRVLKQPVIVDNRAGASGAIAAGMTVSAKPDGYTLYFVASPTMTMTPLIQRSVSFKPAQDFSYVGLITDYTNVLLINKDFPARNVDELVSYARKHPGEVSFGSAGVGASNHLSAELLAQMTGVKMLHVPYKGNAPAMADVISGKVTFMFDITGTAIGHIRGGKVRALAVTSKTRNPALPNVPTLVESGLKQYDLTGWYALVGPPGLPADVTDRLVKAQQAVVQDAAYRERMVDGGYDLNVSGPAALQERIKKELALWGNVVKTANIEVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-10-07" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LGU7</accession>
    <name>Q1LGU7_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_3758</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10629.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3758"/>
    <dbReference type="EnsemblBacteria" id="ABF10629">
        <property type="protein sequence ID" value="ABF10629"/>
        <property type="gene ID" value="Rmet_3758"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3758"/>
    <dbReference type="HOGENOM" id="CLU_2106978_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF10629.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="115" mass="12668" checksum="3E72E30A625AD1D5" modified="2006-05-30" version="1">MRHARFGGQVLLDGLVVFFFEVVEPSGICDQRADGFTPFGCTVLGSVDARALLSPTGAECKVGGGSGKVVRPRYAYFRSEFCRRWNFARRSPLLKLRSSNVQRSALVVWTPVAGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="58" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LGV8</accession>
    <name>Q1LGV8_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_3746</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10618.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518264.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3746"/>
    <dbReference type="EnsemblBacteria" id="ABF10618">
        <property type="protein sequence ID" value="ABF10618"/>
        <property type="gene ID" value="Rmet_3746"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3746"/>
    <dbReference type="HOGENOM" id="CLU_166639_0_0_4"/>
    <dbReference type="OMA" id="THSRWAN"/>
    <dbReference type="OrthoDB" id="2213313at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF10618.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="120" mass="14294" checksum="445B2C38ECA0866A" modified="2006-05-30" version="1">MWMPCHDEFNLMWADKPGTGHYFAPPIHPDPHMYKYVWWVWTRASPWDGTSFFTNTPALSMGEFRQIERQLIDAREHFFVYGIQRPRRGSALERSTPQWAHPIFAPAYDEDEDIAWQGHK</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="72" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LH43</accession>
    <name>Q1LH43_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Tad_C domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_3661</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10533.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518188.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3661"/>
    <dbReference type="EnsemblBacteria" id="ABF10533">
        <property type="protein sequence ID" value="ABF10533"/>
        <property type="gene ID" value="Rmet_3661"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3661"/>
    <dbReference type="eggNOG" id="COG4655">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_469008_0_0_4"/>
    <dbReference type="OMA" id="LSVMDYN"/>
    <dbReference type="OrthoDB" id="1040649at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018705">
        <property type="entry name" value="DUF2134_membrane"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09977">
        <property type="entry name" value="Tad_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="21"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="domain" description="Tad_C" evidence="2">
        <location>
            <begin position="58"/>
            <end position="171"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF09977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF10533.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="575" mass="58363" checksum="1A4942E09AC1AEDA" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LH78</accession>
    <name>Q1LH78_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative ATPase with possible chaperone-like activity (MoxR-like)</fullName>
            <ecNumber evidence="2">3.6.1.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">moxR</name>
        <name evidence="2" type="ordered locus">Rmet_3626</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EC" id="3.6.1.-" evidence="2"/>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10498.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518156.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3626"/>
    <dbReference type="EnsemblBacteria" id="ABF10498">
        <property type="protein sequence ID" value="ABF10498"/>
        <property type="gene ID" value="Rmet_3626"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3626"/>
    <dbReference type="eggNOG" id="COG0714">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_034716_2_0_4"/>
    <dbReference type="OMA" id="FMARVSI"/>
    <dbReference type="OrthoDB" id="895310at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011703">
        <property type="entry name" value="ATPase_AAA-3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041628">
        <property type="entry name" value="ChlI/MoxR_AAA_lid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07726">
        <property type="entry name" value="AAA_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17863">
        <property type="entry name" value="AAA_lid_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA" evidence="1">
        <location>
            <begin position="38"/>
            <end position="179"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF10498.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="308" mass="33139" checksum="ABB6B78707877450" modified="2006-05-30" version="1">MTARPKIAASLTEVQTQLGRIVLGKPRQVRLALACILAGGHLLLEDVPGVGKTTLAHALARTLGLHYQRVQFTSDLLPADLVGVSIFKRETGEFHFHHGPVFAQVVLADEINRAPPKTQSALLEAMAERQVTHDGITETLSAPFFVIATQNPLEQIGTHPLPESQLDRFTMRISLGYPDAAFERVLYLGGAIKDEAEAVMDADQVVALQTAVEKVYASPALVDYVLALVQATRVESSFVTGLSPRAGLALLACARAWALLDQREMVLPEDVQAVFEPVAAHRLLPAGQVALNQGTDTLARLLASVAIP</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-10-07" version="57" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LHB2</accession>
    <name>Q1LHB2_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_3592</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10464.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3592"/>
    <dbReference type="EnsemblBacteria" id="ABF10464">
        <property type="protein sequence ID" value="ABF10464"/>
        <property type="gene ID" value="Rmet_3592"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3592"/>
    <dbReference type="eggNOG" id="ENOG5033PIE">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_590099_0_0_4"/>
    <dbReference type="OMA" id="MIRVGYA"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021340">
        <property type="entry name" value="DUF2957"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11170">
        <property type="entry name" value="DUF2957"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193497" evidence="1">
        <location>
            <begin position="34"/>
            <end position="428"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF10464.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="428" mass="44418" checksum="F8119273B9B17EF5" modified="2006-05-30" version="1">MPLPQSHQPRSAASLRPWAPRLALALVTTAALAGCGGGGDGDSAQTAVPAAVPARLCPASVDYSTPYVGGTGSGELVSVQIDTTKMTWAVTFLDSSVPRTTGTVQPTRSDPTNGQNVVTGKLAQETGLPTDKLNQCAFQLIGASLDASRPARLFLGEGVMGGTIPGARIQFNGIAGAGAVPDTTFPYFQFIGFAQTETDLSKIAGVYNGSGFHEIPSKNFAQVAQDYKMSLAADGSFTVCDNKTGGTCKQKGNKFVPQGNGSLLSTNYEGEQQPSLGGVLGRAYLIVGKLRGQLVPIMIRVGYANASFTNLQPVGADDEIGIGMMAPAVSITQGSVNGEYVGVDSNFDYRTTALVGADAAQLDPFRAADATLATALKLDYSQTTAGVVTTTRKDNATITGKFMFTGGVFGFLENRTTGPYFTVGAFVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="98" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LHH2</accession>
    <name>Q1LHH2_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Transcriptional regulator, AsnC/Lrp family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Rmet_3532</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10404.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517951.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3532"/>
    <dbReference type="EnsemblBacteria" id="ABF10404">
        <property type="protein sequence ID" value="ABF10404"/>
        <property type="gene ID" value="Rmet_3532"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3532"/>
    <dbReference type="eggNOG" id="COG1522">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_091233_0_3_4"/>
    <dbReference type="OMA" id="RLIFQQW"/>
    <dbReference type="OrthoDB" id="1534467at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000485">
        <property type="entry name" value="AsnC-type_HTH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011008">
        <property type="entry name" value="Dimeric_a/b-barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019888">
        <property type="entry name" value="Tscrpt_reg_AsnC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019887">
        <property type="entry name" value="Tscrpt_reg_AsnC/Lrp_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01037">
        <property type="entry name" value="AsnC_trans_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13404">
        <property type="entry name" value="HTH_AsnC-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00033">
        <property type="entry name" value="HTHASNC"/>
    </dbReference>
    <dbReference type="SMART" id="SM00344">
        <property type="entry name" value="HTH_ASNC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54909">
        <property type="entry name" value="SSF54909"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50956">
        <property type="entry name" value="HTH_ASNC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH asnC-type" evidence="4">
        <location>
            <begin position="6"/>
            <end position="67"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50956"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABF10404.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="159" mass="17524" checksum="6528CECC77DF8639" modified="2006-05-30" version="1">MNHADLDPFDLALLAALQADGRSTHQQLAERVHLSPSQVGRRLARLEADGVITGYRVNLSPQALGLGVLVFISVKLAHHGDTIIERFAEEILLLEEVQECYSVAGEADYLIRVVVADLPTLAEFTMKRLMRVPGVESIRSNIVLTALKREGPLPLSHLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="67" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LHH9</accession>
    <name>Q1LHH9_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Copper chaperone, heavy metal ion binding (Modular protein)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">cupC</name>
        <name evidence="3" type="ordered locus">Rmet_3525</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10397.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="SMR" id="Q1LHH9"/>
    <dbReference type="STRING" id="266264.Rmet_3525"/>
    <dbReference type="EnsemblBacteria" id="ABF10397">
        <property type="protein sequence ID" value="ABF10397"/>
        <property type="gene ID" value="Rmet_3525"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3525"/>
    <dbReference type="eggNOG" id="COG2608">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1904990_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00371">
        <property type="entry name" value="HMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017969">
        <property type="entry name" value="Heavy-metal-associated_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006121">
        <property type="entry name" value="HMA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036163">
        <property type="entry name" value="HMA_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00403">
        <property type="entry name" value="HMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55008">
        <property type="entry name" value="SSF55008"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01047">
        <property type="entry name" value="HMA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50846">
        <property type="entry name" value="HMA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HMA" evidence="2">
        <location>
            <begin position="68"/>
            <end position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF10397.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="133" mass="14774" checksum="E85AE062B958815D" modified="2006-05-30" version="1">MRQDGSSESWNLSDGARQLHCEPYHHWKVKDLLSRTRHLRFPWWEGSHFSLSSRFIAHRSNPTGVITMIQFQVEGMSCNHCVGSITRAVQAVDPAARVSADVPTQSVKVESAADRQALQQAIEGAGYPVKSAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="96" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LHI3</accession>
    <name>Q1LHI3_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Choline dehydrogenase, a flavoprotein</fullName>
            <ecNumber evidence="6">1.1.99.1</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">betA2</name>
        <name evidence="6" type="ordered locus">Rmet_3521</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the GMC oxidoreductase family.</text>
    </comment>
    <dbReference type="EC" id="1.1.99.1" evidence="6"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10393.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517942.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3521"/>
    <dbReference type="EnsemblBacteria" id="ABF10393">
        <property type="protein sequence ID" value="ABF10393"/>
        <property type="gene ID" value="Rmet_3521"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3521"/>
    <dbReference type="eggNOG" id="COG2303">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002865_7_1_4"/>
    <dbReference type="OMA" id="NEGVGYF"/>
    <dbReference type="OrthoDB" id="365211at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008812">
        <property type="term" value="F:choline dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012132">
        <property type="entry name" value="GMC_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000172">
        <property type="entry name" value="GMC_OxRdtase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007867">
        <property type="entry name" value="GMC_OxRtase_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11552">
        <property type="entry name" value="PTHR11552"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05199">
        <property type="entry name" value="GMC_oxred_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00732">
        <property type="entry name" value="GMC_oxred_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000137">
        <property type="entry name" value="Alcohol_oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="SSF51905"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00623">
        <property type="entry name" value="GMC_OXRED_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0274">FAD</keyword>
    <keyword evidence="3" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GMC_OxRdtase_N" evidence="5">
        <location>
            <begin position="81"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="535"/>
            <end position="555"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="83"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="219"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010790"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR000137-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS00623"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABF10393.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="555" mass="60733" checksum="8B33E2EABFF1E45B" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LHJ0</accession>
    <name>Q1LHJ0_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Probable membrane transporter protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Rmet_3514</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cell membrane</location>
            <topology evidence="5">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the 4-toluene sulfonate uptake permease (TSUP) (TC 2.A.102) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10386.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517937.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3514"/>
    <dbReference type="EnsemblBacteria" id="ABF10386">
        <property type="protein sequence ID" value="ABF10386"/>
        <property type="gene ID" value="Rmet_3514"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3514"/>
    <dbReference type="eggNOG" id="COG0730">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045498_2_0_4"/>
    <dbReference type="OMA" id="KEQCLAV"/>
    <dbReference type="OrthoDB" id="1445898at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002781">
        <property type="entry name" value="TM_pro_TauE-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01925">
        <property type="entry name" value="TauE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="68"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="98"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="136"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="176"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="199"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="229"/>
            <end position="247"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU363041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABF10386.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="252" mass="26237" checksum="A6EF8BB07C90B03F" modified="2006-05-30" version="1">MEFVFLAAAAFLAGVIDAVAGGGGLVQVPALFSTYPNMSPATLIGTTKVASLAGTANAATRYARKVRIYWGATAPAMLAAFVMAMAGAWTLTKIPAEPLRKALPLVLLVLLIYTVAKKNLGAEHAPTLTGTRERVAALLAGAVIGFYDGAFGPGTGSFLMIVFVRVFGYDFLHASASAKVVNLATNLAALLLLASKGHIWWQLGLIMAAANVAGSQVGSKLALRHGSAFVRKVFIAVVSALILKTAYDAFLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LHR4</accession>
    <name>Q1LHR4_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">CMD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_3440</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10312.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517872.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3440"/>
    <dbReference type="EnsemblBacteria" id="ABF10312">
        <property type="protein sequence ID" value="ABF10312"/>
        <property type="gene ID" value="Rmet_3440"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3440"/>
    <dbReference type="eggNOG" id="COG2128">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_082760_6_2_4"/>
    <dbReference type="OMA" id="CGHCIDM"/>
    <dbReference type="OrthoDB" id="1893149at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051920">
        <property type="term" value="F:peroxiredoxin activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1290.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029032">
        <property type="entry name" value="AhpD-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004675">
        <property type="entry name" value="AhpD_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003779">
        <property type="entry name" value="CMD-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02627">
        <property type="entry name" value="CMD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69118">
        <property type="entry name" value="SSF69118"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00778">
        <property type="entry name" value="ahpD_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CMD" evidence="1">
        <location>
            <begin position="12"/>
            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02627"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF10312.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="152" mass="16268" checksum="FC61E783AD605693" modified="2006-05-30" version="1">MDFQTFTKSASDVYAALSGVSKAVEAAGMDKALTELLKLRVSQINGCAFCLQFHLNIARKLDIDPRKFDLLAGWRDAGIYTAREAAALGWAEALTSMNDPAARDVAFATARTVFSEQELMLLAAAIASINAWNRIAVGLHFAPPPASPGGRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LHW0</accession>
    <name>Q1LHW0_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="9">General secretory pathway component, cryptic</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">gspF</name>
        <name evidence="9" type="ordered locus">Rmet_3394</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the GSP F family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10266.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517829.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3394"/>
    <dbReference type="EnsemblBacteria" id="ABF10266">
        <property type="protein sequence ID" value="ABF10266"/>
        <property type="gene ID" value="Rmet_3394"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3394"/>
    <dbReference type="eggNOG" id="COG1459">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035032_0_1_4"/>
    <dbReference type="OMA" id="VAEQCEK"/>
    <dbReference type="OrthoDB" id="1596769at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015627">
        <property type="term" value="C:type II protein secretion system complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015628">
        <property type="term" value="P:protein secretion by the type II secretion system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.81.30">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003004">
        <property type="entry name" value="GspF/PilC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011850">
        <property type="entry name" value="T2SS_GspF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018076">
        <property type="entry name" value="T2SS_GspF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042094">
        <property type="entry name" value="T2SS_GspF_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30012">
        <property type="entry name" value="PTHR30012"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00482">
        <property type="entry name" value="T2SSF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00812">
        <property type="entry name" value="BCTERIALGSPF"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02120">
        <property type="entry name" value="GspF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="170"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="224"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="373"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="domain" description="T2SSF" evidence="8">
        <location>
            <begin position="71"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="domain" description="T2SSF" evidence="8">
        <location>
            <begin position="274"/>
            <end position="396"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00482"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ABF10266.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="405" mass="43797" checksum="B3D4330AB4B5AC17" modified="2006-05-30" version="1">MPAYRYEAADAAGRADRGVIEADSPKQARAQLRARGLTPIIVDPLAGAGLAKRSGSSAFVRRLSTQEQALFTRQLASLIVSGLPLDEALGALADQAERQYVHELLAGIRAEVMGGSSLSVALAQHPKDFPDIYRALVSAGEHSGHLGMVLERLATYIETRNTLTSKIRLAFTYPAIVTVVAFAIVIFLLSYVVPQVVSVFANTKQKLPTLTVIMLALSDFVRNWWWAALGVIAAVVAIIRSLLKQPDVRLAWHRWLLTAPLVGKLIRGYNTARFASTLSILVSAGVPILRSLQAAGETLTNQALKANVEDATTRVREGASLARALAAQNQFPPVLVHLIRSGEATGNLPTMLDRAATGEGQELERRTLFLTSLLEPLLILTMGVVVLMIVLAVLMPIIEINQLVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-10-07" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LIK2</accession>
    <name>Q1LIK2_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_3152</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10024.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3152"/>
    <dbReference type="EnsemblBacteria" id="ABF10024">
        <property type="protein sequence ID" value="ABF10024"/>
        <property type="gene ID" value="Rmet_3152"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3152"/>
    <dbReference type="HOGENOM" id="CLU_2540128_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF10024.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="83" mass="9108" checksum="61921CC6297B6528" modified="2006-05-30" version="1">MLLLGSASPRDLASLRVLSDPGGGTRRHIIIEYNRSQTISPGWECSDEAVAPQSHGETWKRRSSIRPDAGPFAVLHCPHRAPH</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="98" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LIL7</accession>
    <name>Q1LIL7_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1 7">Transcriptional regulator MraZ</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7 10" type="primary">mraZ</name>
        <name evidence="10" type="ordered locus">Rmet_3137</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="7">Forms oligomers.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
            <location evidence="7">Nucleoid</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the MraZ family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10009.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3137"/>
    <dbReference type="EnsemblBacteria" id="ABF10009">
        <property type="protein sequence ID" value="ABF10009"/>
        <property type="gene ID" value="Rmet_3137"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3137"/>
    <dbReference type="eggNOG" id="COG2001">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_107907_2_1_4"/>
    <dbReference type="OMA" id="RGQERCL"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009295">
        <property type="term" value="C:nucleoid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd16321">
        <property type="entry name" value="MraZ_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16320">
        <property type="entry name" value="MraZ_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1550.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01008">
        <property type="entry name" value="MraZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003444">
        <property type="entry name" value="MraZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035644">
        <property type="entry name" value="MraZ_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020603">
        <property type="entry name" value="MraZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035642">
        <property type="entry name" value="MraZ_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038619">
        <property type="entry name" value="MraZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007159">
        <property type="entry name" value="SpoVT-AbrB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037914">
        <property type="entry name" value="SpoVT-AbrB_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34701">
        <property type="entry name" value="PTHR34701"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02381">
        <property type="entry name" value="MraZ"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89447">
        <property type="entry name" value="SSF89447"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00242">
        <property type="entry name" value="TIGR00242"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51740">
        <property type="entry name" value="SPOVT_ABRB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 7 8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 7" id="KW-0804">Transcription</keyword>
    <keyword evidence="4 7" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="SpoVT-AbrB" evidence="9">
        <location>
            <begin position="14"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="domain" description="SpoVT-AbrB" evidence="9">
        <location>
            <begin position="86"/>
            <end position="129"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00013860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51740"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ABF10009.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="151" mass="16540" checksum="54D248C705E2E932" modified="2006-05-30" version="1">MPTATRGASLFQGASALSLDAKGRMSIPARHREALQTQAEGRVTLTKHPDGCLLLFPRPEWEVFRGRIAALPMDAHWWKRIFLGNAADVDMDGAGRVLIAPELRSAAMLDKEVMLLGMGSHFEVWDAATYAAKEQAAMAQGMPEALKNFSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="99" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LIP6</accession>
    <name>Q1LIP6_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Type IV pilus assembly protein PilB</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">pilB</name>
        <name evidence="5" type="ordered locus">Rmet_3108</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the GSP E family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09980.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517604.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3108"/>
    <dbReference type="EnsemblBacteria" id="ABF09980">
        <property type="protein sequence ID" value="ABF09980"/>
        <property type="gene ID" value="Rmet_3108"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3108"/>
    <dbReference type="eggNOG" id="COG2804">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013446_10_1_4"/>
    <dbReference type="OMA" id="NMGVPAF"/>
    <dbReference type="OrthoDB" id="749544at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009297">
        <property type="term" value="P:pilus assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.160">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013374">
        <property type="entry name" value="ATPase_typ4_pilus-assembl_PilB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001482">
        <property type="entry name" value="T2SS/T4SS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037257">
        <property type="entry name" value="T2SS_E_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007831">
        <property type="entry name" value="T2SS_GspE_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00437">
        <property type="entry name" value="T2SSE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05157">
        <property type="entry name" value="T2SSE_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160246">
        <property type="entry name" value="SSF160246"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02538">
        <property type="entry name" value="type_IV_pilB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00662">
        <property type="entry name" value="T2SP_E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="T2SP_E" evidence="4">
        <location>
            <begin position="391"/>
            <end position="405"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006611"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS00662"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABF09980.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="573" mass="62953" checksum="44F690FB9630E01E" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LJ26</accession>
    <name>Q1LJ26_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Transcriptional regulator, LysR-family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">phcA</name>
        <name evidence="3" type="ordered locus">Rmet_2977</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the LysR transcriptional regulatory family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09850.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517511.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2977"/>
    <dbReference type="EnsemblBacteria" id="ABF09850">
        <property type="protein sequence ID" value="ABF09850"/>
        <property type="gene ID" value="Rmet_2977"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2977"/>
    <dbReference type="eggNOG" id="COG0583">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039613_39_0_4"/>
    <dbReference type="OMA" id="DYWRIPD"/>
    <dbReference type="OrthoDB" id="1591366at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005119">
        <property type="entry name" value="LysR_subst-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000847">
        <property type="entry name" value="Tscrpt_reg_HTH_LysR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00126">
        <property type="entry name" value="HTH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03466">
        <property type="entry name" value="LysR_substrate"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50931">
        <property type="entry name" value="HTH_LYSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH lysR-type" evidence="2">
        <location>
            <begin position="4"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF09850.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="333" mass="37182" checksum="3686C73CCA0A6233" modified="2006-05-30" version="1">MVNVDTKLLVIFTELLSKRNATYVAEKMHMTAPAVSHSLGRLREIFDDPLFIRVPHGLTPTPRALELGPKIRDMLDLWSSINEGDADNFDPAIATGTLNIGFAAELGDTVFNRFVLRIKQLAPDLHIRLVESHSWEADVASMRANELDLAFSPFPTRHPEIVEEVVTSLNLWVCARKNHPLLKNDCTLEQYLDCSHIFMAHSGGAGRPAPALIPLDYALQQRGLKRNATLTVHSWRAQAELASQTDMIFTVNALTKDMACETYGLKAYPLPAELNTMLGLNMFWHRSRNTHPMLVWARGLFRQVVGEFLGLPAAAAAVPRSLRVVAEQDLTQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LJL7</accession>
    <name>Q1LJL7_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 8">Corrinoid adenosyltransferase</fullName>
            <ecNumber evidence="5 8">2.5.1.17</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Cob(II)alamin adenosyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">Cob(II)yrinic acid a,c-diamide adenosyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">btuR</name>
        <name evidence="11" type="ordered locus">Rmet_2786</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7 8">Required for both de novo synthesis of the corrin ring for the assimilation of exogenous corrinoids. Participates in the adenosylation of a variety of incomplete and complete corrinoids.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2 8">
            <text>2 ATP + 2 cob(II)alamin + reduced [electron-transfer flavoprotein] = 2 adenosylcob(III)alamin + 3 H(+) + oxidized [electron-transfer flavoprotein] + 2 triphosphate</text>
            <dbReference type="Rhea" id="RHEA:28671"/>
            <dbReference type="Rhea" id="RHEA-COMP:10685"/>
            <dbReference type="Rhea" id="RHEA-COMP:10686"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16304"/>
            <dbReference type="ChEBI" id="CHEBI:18036"/>
            <dbReference type="ChEBI" id="CHEBI:18408"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
            <dbReference type="ChEBI" id="CHEBI:58307"/>
            <dbReference type="EC" id="2.5.1.17"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 8">
            <text>2 ATP + 2 cob(II)yrinate a,c diamide + reduced [electron-transfer flavoprotein] = 2 adenosylcob(III)yrinate a,c-diamide + 3 H(+) + oxidized [electron-transfer flavoprotein] + 2 triphosphate</text>
            <dbReference type="Rhea" id="RHEA:11528"/>
            <dbReference type="Rhea" id="RHEA-COMP:10685"/>
            <dbReference type="Rhea" id="RHEA-COMP:10686"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:18036"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
            <dbReference type="ChEBI" id="CHEBI:58307"/>
            <dbReference type="ChEBI" id="CHEBI:58503"/>
            <dbReference type="ChEBI" id="CHEBI:58537"/>
            <dbReference type="EC" id="2.5.1.17"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3 8">Cofactor biosynthesis; adenosylcobalamin biosynthesis; adenosylcobalamin from cob(II)yrinate a,c-diamide: step 2/7.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 8">Belongs to the Cob(I)alamin adenosyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.17" evidence="5 8"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09659.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517357.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2786"/>
    <dbReference type="EnsemblBacteria" id="ABF09659">
        <property type="protein sequence ID" value="ABF09659"/>
        <property type="gene ID" value="Rmet_2786"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2786"/>
    <dbReference type="eggNOG" id="COG2109">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_088595_0_0_4"/>
    <dbReference type="OMA" id="HAMGEGF"/>
    <dbReference type="OrthoDB" id="1690483at2"/>
    <dbReference type="UniPathway" id="UPA00148">
        <property type="reaction ID" value="UER00233"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008817">
        <property type="term" value="F:cob(I)yrinic acid a,c-diamide adenosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009236">
        <property type="term" value="P:cobalamin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006779">
        <property type="term" value="P:porphyrin-containing compound biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00561">
        <property type="entry name" value="CobA_CobO_BtuR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003724">
        <property type="entry name" value="CblAdoTrfase_CobA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025826">
        <property type="entry name" value="Co_AT_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12557">
        <property type="entry name" value="Co_AT_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02572">
        <property type="entry name" value="CobA_CobO_BtuR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015617">
        <property type="entry name" value="Adensltrnsf_CobA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00708">
        <property type="entry name" value="cobA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0169">Cobalamin biosynthesis</keyword>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6 8" id="KW-0627">Porphyrin biosynthesis</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 11" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Co_AT_N" evidence="10">
        <location>
            <begin position="8"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000143"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00024929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRNR" id="PIRNR015617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF12557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ABF09659.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="209" mass="23081" checksum="CBC6BC4415BF6E7D" modified="2006-05-30" version="1">MSDTPNTNDDAGRDARHKARMVRKKEVVDSKIAAAQNERGVIVITTGNGKGKSSSGFGMVVRAMGHGMRTGVVQFIKGAFPSGEEMFLRRFPEECEFHVMGEGYTWETQDRARDVAKAEAAWEVARRMLRDPSFDLILFDELNIALKLHYLDLDTVIADLRARPPKQHVVITGRGAPSALIEAADTVTDMTPVKHAFEQGIKAQPGVEM</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="61" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LJW9</accession>
    <name>Q1LJW9_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative Type IV pilus assembly protein (PilX-like)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_2684</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09557.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517257.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2684"/>
    <dbReference type="EnsemblBacteria" id="ABF09557">
        <property type="protein sequence ID" value="ABF09557"/>
        <property type="gene ID" value="Rmet_2684"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2684"/>
    <dbReference type="eggNOG" id="COG4726">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1522715_0_0_4"/>
    <dbReference type="OMA" id="CMAALPR"/>
    <dbReference type="OrthoDB" id="2035332at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025205">
        <property type="entry name" value="PilX/PilW_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13681">
        <property type="entry name" value="PilX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PilX" evidence="1">
        <location>
            <begin position="92"/>
            <end position="186"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13681"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF09557.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="186" mass="19642" checksum="DBED46F085A63653" modified="2006-05-30" version="1">MLNLRAHSGAALLAFLSTLLLMMASIGAAAAHLLFAGRSMTSQWLDREIAFRAAEVALLDAEADLIAAIAEVGGERLASWPTPGTCGTGAQRGLCMADGDMTAWMPWLEGNLPADLGIDAGTFTGITMPILPADVIGATTAPRYVIEPLEDRTGLTADAWPRFRITALGFGRDTGVRALLQTEFQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="95" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LK33</accession>
    <name>Q1LK33_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Geranyltranstransferase</fullName>
            <ecNumber evidence="3">2.5.1.1</ecNumber>
            <ecNumber evidence="3">2.5.1.10</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">ispA</name>
        <name evidence="3" type="ordered locus">Rmet_2616</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the FPP/GGPP synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.1" evidence="3"/>
    <dbReference type="EC" id="2.5.1.10" evidence="3"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09493.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517196.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2616"/>
    <dbReference type="EnsemblBacteria" id="ABF09493">
        <property type="protein sequence ID" value="ABF09493"/>
        <property type="gene ID" value="Rmet_2616"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2616"/>
    <dbReference type="eggNOG" id="COG0142">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014015_0_1_4"/>
    <dbReference type="OMA" id="CEGQALD"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004161">
        <property type="term" value="F:dimethylallyltranstransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004337">
        <property type="term" value="F:geranyltranstransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008299">
        <property type="term" value="P:isoprenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00685">
        <property type="entry name" value="Trans_IPPS_HT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.600.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008949">
        <property type="entry name" value="Isoprenoid_synthase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000092">
        <property type="entry name" value="Polyprenyl_synt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033749">
        <property type="entry name" value="Polyprenyl_synt_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00348">
        <property type="entry name" value="polyprenyl_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48576">
        <property type="entry name" value="SSF48576"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00723">
        <property type="entry name" value="POLYPRENYL_SYNTHASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00444">
        <property type="entry name" value="POLYPRENYL_SYNTHASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU004466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF09493.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="294" mass="30782" checksum="1EBA590AD4E8CB81" modified="2006-05-30" version="1">MSDFATWMRAQGARTEAALEAALPSTDTIPYTLHEAMRYAVLGGGKRVRPLLVHAAGEVVGATPEACDAAACAVEMIHAYSLVHDDMPCMDDDDLRRGRPTVHKAYDEATALLVGDALQSQAFIVLAQTPALAAEARLKVVAELAVASGSIGMCGGQAIDLQNVGKAMTREALEGMHRMKTGALLRASVRMGALCGNIDQAGLVALDRYAAAVGLAFQVVDDILDVTADTATLGKTAGKDAANDKPTYVSLLGLDAARELAAQLRTDAHEALEGFGTRAGRLAELADLIVLRSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="112" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LKK0</accession>
    <name>Q1LKK0_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 8 9">Nicotinate phosphoribosyltransferase</fullName>
            <shortName evidence="8">NAPRTase</shortName>
            <ecNumber evidence="4 8 9">6.3.4.21</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8 12" type="primary">pncB</name>
        <name evidence="12" type="ordered locus">Rmet_2449</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8 9">Catalyzes the synthesis of beta-nicotinate D-ribonucleotide from nicotinate and 5-phospho-D-ribose 1-phosphate at the expense of ATP.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 8 9">
            <text>5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O + nicotinate = ADP + diphosphate + nicotinate beta-D-ribonucleotide + phosphate</text>
            <dbReference type="Rhea" id="RHEA:36163"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:32544"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57502"/>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.4.21"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 8 9">Cofactor biosynthesis; NAD(+) biosynthesis; nicotinate D-ribonucleotide from nicotinate: step 1/1.</text>
    </comment>
    <comment type="PTM">
        <text evidence="8 9">Transiently phosphorylated on a His residue during the reaction cycle. Phosphorylation strongly increases the affinity for substrates and increases the rate of nicotinate D-ribonucleotide production. Dephosphorylation regenerates the low-affinity form of the enzyme, leading to product release.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 8 9">Belongs to the NAPRTase family.</text>
    </comment>
    <dbReference type="EC" id="6.3.4.21" evidence="4 8 9"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09326.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2449"/>
    <dbReference type="EnsemblBacteria" id="ABF09326">
        <property type="protein sequence ID" value="ABF09326"/>
        <property type="gene ID" value="Rmet_2449"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2449"/>
    <dbReference type="eggNOG" id="COG1488">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030991_1_0_4"/>
    <dbReference type="OMA" id="QAVFHRY"/>
    <dbReference type="UniPathway" id="UPA00253">
        <property type="reaction ID" value="UER00457"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004516">
        <property type="term" value="F:nicotinate phosphoribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004514">
        <property type="term" value="F:nicotinate-nucleotide diphosphorylase (carboxylating) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009435">
        <property type="term" value="P:NAD biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01401">
        <property type="entry name" value="PncB_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00570">
        <property type="entry name" value="NAPRTase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041525">
        <property type="entry name" value="N/Namide_PRibTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040727">
        <property type="entry name" value="NAPRTase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006406">
        <property type="entry name" value="Nic_PRibTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007229">
        <property type="entry name" value="Nic_PRibTrfase-Fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036068">
        <property type="entry name" value="Nicotinate_pribotase-like_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11098">
        <property type="entry name" value="PTHR11098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04095">
        <property type="entry name" value="NAPRTase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17767">
        <property type="entry name" value="NAPRTase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000484">
        <property type="entry name" value="NAPRT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51690">
        <property type="entry name" value="SSF51690"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01514">
        <property type="entry name" value="NAPRTase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="6 8 9" id="KW-0436">Ligase</keyword>
    <keyword evidence="5 8" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="7 8 9" id="KW-0662">Pyridine nucleotide biosynthesis</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="NAPRTase_N" evidence="11">
        <location>
            <begin position="14"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="domain" description="NAPRTase" evidence="10">
        <location>
            <begin position="171"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="modified residue" description="Phosphohistidine; by autocatalysis" evidence="8">
        <location>
            <position position="223"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004952"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010897"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00570"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF04095"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF17767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ABF09326.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="399" mass="45786" checksum="AAE948040B548617" modified="2006-05-30" version="1">MPPSGLVMIIRSLLDTDLYKFTMMQVVLHHFPQAQVEYRFKCRNAGVDLTPYVDEIRSQITQLCQLRFTDDELNYLRGLRFIKSDFVEFLALFHLNEKYVQVLPSAKGNGEIEIIIKGPWLHTILFEIPLLAIVNEVYFRRTQPKPDLAEGRRRLQAKLELLAAPPYVDCVIADYGTRRRFSCDWQEEVLLSMRDAIGPQLAGTSNVHFARLHNMTPLGTMAHEYLQACQALGPRLRDSQVYALERWAHEYRGDLGIALSDTYGFDAFLRDFDLYFCKLFDGVRHDSGDPFEWGERMIAHYAANRVDPRGKTLIFSDALDIPKVIELYERFRGRCKLAFGVGTNLTNDLGYKPLQIVIKMVRCNGQPVAKLSDTPEKTMCDDPGYLSYLRQVFAVQAPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LKL3</accession>
    <name>Q1LKL3_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Peptidase S49, periplasmic serine protease (ClpP class)</fullName>
            <ecNumber evidence="5">3.4.-.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">sohB</name>
        <name evidence="5" type="ordered locus">Rmet_2436</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EC" id="3.4.-.-" evidence="5"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09313.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517030.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2436"/>
    <dbReference type="EnsemblBacteria" id="ABF09313">
        <property type="protein sequence ID" value="ABF09313"/>
        <property type="gene ID" value="Rmet_2436"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2436"/>
    <dbReference type="eggNOG" id="COG0616">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046540_1_1_4"/>
    <dbReference type="OMA" id="YVYDEIR"/>
    <dbReference type="OrthoDB" id="1318587at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008233">
        <property type="term" value="F:peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd07023">
        <property type="entry name" value="S49_Sppa_N_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029045">
        <property type="entry name" value="ClpP/crotonase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002142">
        <property type="entry name" value="Peptidase_S49"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01343">
        <property type="entry name" value="Peptidase_S49"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52096">
        <property type="entry name" value="SSF52096"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0645">Protease</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="102"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase_S49" evidence="4">
        <location>
            <begin position="201"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="16"/>
            <end position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01343"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABF09313.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="387" mass="41520" checksum="C0EAA3D5B0E6BBE7" modified="2006-05-30" version="1">MTEQQNPPNEPGQPQERAGDDSKLETAKHAEFPLERELQAGDDAAKREADERRARLAGKGGPVGGSSGSGGGSGSGGWERDVLEKVLMASLREQRSARRWKIFFRFLTLAIVALILYAVIDFKGDVLSSTSGRHTAMVTLDGEIAAGTTASADSINAALQAAFEDTSAAGVILKINSPGGSPVQAGIINDEIHRLRKLYPDKPFYVVVEEICASGGYYVAAAADKIYVDKASIVGSIGVLMDGFGFTGLMDKVGVQRRLYTSGANKGMLDPFSPEVPRQKGYAESMLKEIHQQFIDVVKEGRGDRLKNDPELFSGLFWSGERAVELGLADGLGSADYVARDLFKAEDIVDYTVKENIAERMAKRFGAAAGSAAMKLMMMSATVPSVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LKT0</accession>
    <name>Q1LKT0_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Major facilitator superfamily MFS_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Rmet_2369</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09246.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004350634.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2369"/>
    <dbReference type="EnsemblBacteria" id="ABF09246">
        <property type="protein sequence ID" value="ABF09246"/>
        <property type="gene ID" value="Rmet_2369"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2369"/>
    <dbReference type="eggNOG" id="COG2814">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047644_2_0_4"/>
    <dbReference type="OMA" id="WNFGLVS"/>
    <dbReference type="OrthoDB" id="1121832at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="SSF103473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="21"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="59"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="83"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="111"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="144"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="172"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="221"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="261"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="286"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="311"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="345"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="376"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="domain" description="MFS" evidence="6">
        <location>
            <begin position="213"/>
            <end position="408"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABF09246.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="408" mass="41852" checksum="C676F01AEC5CE121" modified="2006-05-30" version="1">MTPPASLPPPAATDARRNVGLLMAAQSLGGAAPPIIISLGGIVGQMLASNPSLATLPVSLYNLGLALSTIPAALLMRRLGRRAAYALGALLGSVSGLIAALGVLWGSFETFCVGTAMAGFYGACVQSYRFAASDAVPPPQRATVISRIMIGGLIAAVIGPQVVIWTRDAWPMAPFAGSFLGQAGLALLALPLLLMLRMPPPQASAVVGAARPLAVIARSPGFVVAVTAGIVSYGLMAFIMTAAPMAMVGCGHTVGEAALGIQWHVLAMFAPSFFTGHLIARFGKTAITACGLVLIGTSGLLALAGLELLHFWGSLILLGVGWNFGFIGATALVTDCYTAPERAKVQALNDFLVFGTVAVASFGSGRLLNTSGWETINGLMLPLIALVLALLGWLAWRNRRQSAAAAAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="59" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LL09</accession>
    <name>Q1LL09_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_2288</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09167.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_003098991.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2288"/>
    <dbReference type="EnsemblBacteria" id="ABF09167">
        <property type="protein sequence ID" value="ABF09167"/>
        <property type="gene ID" value="Rmet_2288"/>
    </dbReference>
    <dbReference type="GeneID" id="56409797"/>
    <dbReference type="KEGG" id="rme:Rmet_2288"/>
    <dbReference type="eggNOG" id="ENOG502Z7W7">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_102169_0_0_4"/>
    <dbReference type="OMA" id="WNECRLY"/>
    <dbReference type="OrthoDB" id="1210691at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="136"/>
            <end position="163"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF09167.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="248" mass="28095" checksum="8A4A920880309BF6" modified="2006-05-30" version="1">MHKDISTSKVFYRPIEAAIRWAGLLRYLPMILATIASPRVLPRSLNCPRWNECRLHSERIYDGILNGELPYGKNGITLNDPNLLNSLDLTVRHVDLKRWMRTHYPEHRPGFLFSRGERMAHPFITMETGQAILVERLALQAALEQARREMRELQEQHDTLLKQSSVLLASKQCEISERAETTYLNIIGGMLTLMLSQSPSGVPYSSFKTQEALVSALVAHYGGTMGITERTLNGKFANAKRYVRSASA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LL75</accession>
    <name>Q1LL75_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Cytochrome c domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Rmet_2222</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09101.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516929.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2222"/>
    <dbReference type="EnsemblBacteria" id="ABF09101">
        <property type="protein sequence ID" value="ABF09101"/>
        <property type="gene ID" value="Rmet_2222"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2222"/>
    <dbReference type="eggNOG" id="COG2010">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_043372_0_0_4"/>
    <dbReference type="OMA" id="EHGASTM"/>
    <dbReference type="OrthoDB" id="1134331at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.760.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009056">
        <property type="entry name" value="Cyt_c-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036909">
        <property type="entry name" value="Cyt_c-like_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13442">
        <property type="entry name" value="Cytochrome_CBB3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46626">
        <property type="entry name" value="SSF46626"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51007">
        <property type="entry name" value="CYTC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 4" id="KW-0349">Heme</keyword>
    <keyword evidence="3 4" id="KW-0408">Iron</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="2 4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="64"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="91"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="domain" description="Cytochrome c" evidence="6">
        <location>
            <begin position="129"/>
            <end position="220"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABF09101.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="395" mass="42571" checksum="BEE0CA5DD17EFBC2" modified="2006-05-30" version="1">MNGPPAQAWLNTLLTLLQDGQLALLRLWHVLHLSGDAHGQPAWPWARRIAGETLLIDLGLARQVAWSLAAISVALACLAVASIWRGKRLTWLAAAALTLILAPWPSPALLFTPAAPTSFHRSPTRFSTDSIAHGARLYAQHCTACHGEDGRGEGPLAAGLLRWPPTFVGPLLGRRADGDLFWHIANGMRDSDGRQTMPAFSRKLDDADTWAVIEYTKALAAGTAASTFGTWPIPMRLFDVTVRCGQDGGRAMADWRGHQRVRVVAFEGDAGGLPLEDPRFLTLLLTRDGKPLVNVPQFRAGCVAASPLAWDAYARIAGIAPNRLAGTEFLADRGGWLRARSLPGRDAWGTADLLCSTGQVNKDRGPTSPGTDGLTSLLLRMDAEPVRFVQGGFIH</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="96" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LL88</accession>
    <name>Q1LL88_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 6">DNA polymerase III subunit epsilon</fullName>
            <ecNumber evidence="3 6">2.7.7.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6 8" type="primary">dnaQ</name>
        <name evidence="8" type="ordered locus">Rmet_2209</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. The epsilon subunit contain the editing function and is a proofreading 3'-5' exonuclease.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5 6">
            <text>a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) = diphosphate + DNA(n+1)</text>
            <dbReference type="Rhea" id="RHEA:22508"/>
            <dbReference type="Rhea" id="RHEA-COMP:17339"/>
            <dbReference type="Rhea" id="RHEA-COMP:17340"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61560"/>
            <dbReference type="ChEBI" id="CHEBI:173112"/>
            <dbReference type="EC" id="2.7.7.7"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 6">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 6">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="6">DNA polymerase III contains a core (composed of alpha, epsilon and theta chains) that associates with a tau subunit. This core dimerizes to form the POLIII' complex. PolIII' associates with the gamma complex (composed of gamma, delta, delta', psi and chi chains) and with the beta chain to form the complete DNA polymerase III complex.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.7" evidence="3 6"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09088.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516916.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2209"/>
    <dbReference type="EnsemblBacteria" id="ABF09088">
        <property type="protein sequence ID" value="ABF09088"/>
        <property type="gene ID" value="Rmet_2209"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2209"/>
    <dbReference type="eggNOG" id="COG0847">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047806_2_0_4"/>
    <dbReference type="OMA" id="FHVYLNP"/>
    <dbReference type="OrthoDB" id="1985371at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003887">
        <property type="term" value="F:DNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004527">
        <property type="term" value="F:exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06131">
        <property type="entry name" value="DNA_pol_III_epsilon_Ecoli_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006054">
        <property type="entry name" value="DnaQ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006309">
        <property type="entry name" value="DnaQ_proteo"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013520">
        <property type="entry name" value="Exonuclease_RNaseT/DNA_pol3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00929">
        <property type="entry name" value="RNase_T"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00479">
        <property type="entry name" value="EXOIII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="SSF53098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00573">
        <property type="entry name" value="dnaq"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01406">
        <property type="entry name" value="dnaQ_proteo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0235">DNA replication</keyword>
    <keyword evidence="6" id="KW-0239">DNA-directed DNA polymerase</keyword>
    <keyword evidence="6" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6" id="KW-0464">Manganese</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0540">Nuclease</keyword>
    <keyword evidence="6 8" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Exonuclease" evidence="7">
        <location>
            <begin position="2"/>
            <end position="174"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00020352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00024632"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU364087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ABF09088.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="241" mass="26591" checksum="134A9FC0A40F0974" modified="2006-05-30" version="1">MRQIVLDTETTGLNHATGDRLIEIGCVELVNRRLTGRHLHFYVNPERDIHEDAIAVHGITLDFLADKPKFAEIVNDVRDFVQDAELIIHNAPFDLGFLDMEFQRLDLPPFRQHASNVIDTLREARQMFPGKRNSLDALCDRLGVSNSHRTLHGALLDAELLAEVYLAMTRGQNSLVIDMLDGAATDGETRSTADLSAMTLPVLLASEAEISAHMGVLKELDKASGGKTVWQEASPAEAATQ</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2007-04-03" modified="2022-08-03" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LLA8</accession>
    <name>RLME_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Ribosomal RNA large subunit methyltransferase E</fullName>
            <ecNumber evidence="1">2.1.1.166</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">23S rRNA Um2552 methyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">rRNA (uridine-2'-O-)-methyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rlmE</name>
        <name evidence="1" type="synonym">ftsJ</name>
        <name evidence="1" type="synonym">rrmJ</name>
        <name type="ordered locus">Rmet_2189</name>
    </gene>
    <organism>
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Specifically methylates the uridine in position 2552 of 23S rRNA at the 2'-O position of the ribose in the fully assembled 50S ribosomal subunit.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-adenosyl-L-methionine + uridine(2552) in 23S rRNA = 2'-O-methyluridine(2552) in 23S rRNA + H(+) + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:42720"/>
            <dbReference type="Rhea" id="RHEA-COMP:10202"/>
            <dbReference type="Rhea" id="RHEA-COMP:10203"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="ChEBI" id="CHEBI:74478"/>
            <dbReference type="EC" id="2.1.1.166"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class I-like SAM-binding methyltransferase superfamily. RNA methyltransferase RlmE family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.166" evidence="1"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09068.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516896.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q1LLA8"/>
    <dbReference type="SMR" id="Q1LLA8"/>
    <dbReference type="STRING" id="266264.Rmet_2189"/>
    <dbReference type="EnsemblBacteria" id="ABF09068">
        <property type="protein sequence ID" value="ABF09068"/>
        <property type="gene ID" value="Rmet_2189"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2189"/>
    <dbReference type="eggNOG" id="COG0293">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009422_4_1_4"/>
    <dbReference type="OMA" id="HRQTDHL"/>
    <dbReference type="OrthoDB" id="1798571at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008650">
        <property type="term" value="F:rRNA (uridine-2'-O-)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01547">
        <property type="entry name" value="RNA_methyltr_E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002877">
        <property type="entry name" value="RNA_MeTrfase_FtsJ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015507">
        <property type="entry name" value="rRNA-MeTfrase_E"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01728">
        <property type="entry name" value="FtsJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005461">
        <property type="entry name" value="23S_rRNA_mtase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0489">Methyltransferase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0698">rRNA processing</keyword>
    <keyword id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000282781" description="Ribosomal RNA large subunit methyltransferase E">
        <location>
            <begin position="1"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="1">
        <location>
            <position position="174"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="60"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="62"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="89"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="105"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="134"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01547"/>
        </source>
    </evidence>
    <sequence length="221" mass="24543" checksum="E5982BC3B42E96FC" modified="2006-05-30" version="1">MAKNKFNQSWLHDHINDPYVKMAQREGYRARAAYKLKEIDDQDKLIQPGQVIVDLGAAPGSWSQYARNKLAASPRAKDGRIDGAVVAIDILPMEPVADVTFIQGDFREEEVFRQLEQVVLDASGGSRIDLVISDMAPNLSGVASADAARIEYLCDLALDFAQAHLKPEGSLLVKCFHGSGYSQIVEKFKRQFKVVASRKPKASRDKSSETFILGRYLKSVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LLE2</accession>
    <name>Q1LLE2_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Cytochrome c family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Rmet_2155</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09034.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2155"/>
    <dbReference type="EnsemblBacteria" id="ABF09034">
        <property type="protein sequence ID" value="ABF09034"/>
        <property type="gene ID" value="Rmet_2155"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2155"/>
    <dbReference type="eggNOG" id="COG2010">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG3258">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_383431_0_0_4"/>
    <dbReference type="OMA" id="KTGQNRF"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.760.10">
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009056">
        <property type="entry name" value="Cyt_c-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036909">
        <property type="entry name" value="Cyt_c-like_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00034">
        <property type="entry name" value="Cytochrom_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13442">
        <property type="entry name" value="Cytochrome_CBB3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46626">
        <property type="entry name" value="SSF46626"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51007">
        <property type="entry name" value="CYTC"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 4" id="KW-0349">Heme</keyword>
    <keyword evidence="3 4" id="KW-0408">Iron</keyword>
    <keyword evidence="2 4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193274" evidence="5">
        <location>
            <begin position="30"/>
            <end position="728"/>
        </location>
    </feature>
    <feature type="domain" description="Cytochrome c" evidence="6">
        <location>
            <begin position="62"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="domain" description="Cytochrome c" evidence="6">
        <location>
            <begin position="209"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="domain" description="Cytochrome c" evidence="6">
        <location>
            <begin position="359"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="domain" description="Cytochrome c" evidence="6">
        <location>
            <begin position="613"/>
            <end position="706"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABF09034.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="728" mass="78765" checksum="16BE7EE25DECE03C" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LLG1</accession>
    <name>Q1LLG1_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_2136</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09015.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516845.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2136"/>
    <dbReference type="EnsemblBacteria" id="ABF09015">
        <property type="protein sequence ID" value="ABF09015"/>
        <property type="gene ID" value="Rmet_2136"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2136"/>
    <dbReference type="eggNOG" id="COG3228">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_063037_0_1_4"/>
    <dbReference type="OMA" id="EHSGEAW"/>
    <dbReference type="OrthoDB" id="1806453at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008237">
        <property type="term" value="F:metallopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="CDD" id="cd20169">
        <property type="entry name" value="Peptidase_M90_mtfA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.390.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024079">
        <property type="entry name" value="MetalloPept_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010384">
        <property type="entry name" value="MtfA_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042252">
        <property type="entry name" value="MtfA_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30164">
        <property type="entry name" value="PTHR30164"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06167">
        <property type="entry name" value="Peptidase_M90"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF09015.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="296" mass="33014" checksum="F14DCB0BACDA18D9" modified="2006-05-30" version="1">MLGKLTSFLRARSKARKLAHYAIPDDLWAQTVAALPFVQRYDEADLTALRELATLFIANKEYSTAHDMELTDEMVVSVAAQACVPILKLGIEWYRGWHGIVLYPGEFVIRKTIQEDVGFEDIGLVHGVEEEASGEAWEHGPVILSWPDVASPGAGLDAHHGEHELPVDTYNVVIHEFAHKLDMLNGEADGVPPFSRVLHPGVDAEQWAETFLSEYDTFAEACDALPDHIWDDPDRLPPRLQVIDPYGCEAPSEFFAVASEAFFVEPIGLRRQWPTLYDALTLFYRQNPAGNAASDT</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="102" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LLQ3</accession>
    <name>Q1LLQ3_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Cytochrome-c oxidase</fullName>
            <ecNumber evidence="5">7.1.1.9</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">ccoN</name>
        <name evidence="14" type="ordered locus">Rmet_2044</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>4 Fe(II)-[cytochrome c] + 8 H(+)(in) + O2 = 4 Fe(III)-[cytochrome c] + 4 H(+)(out) + 2 H2O</text>
            <dbReference type="Rhea" id="RHEA:11436"/>
            <dbReference type="Rhea" id="RHEA-COMP:10350"/>
            <dbReference type="Rhea" id="RHEA-COMP:14399"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
            <dbReference type="EC" id="7.1.1.9"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>Cu(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29036"/>
        </cofactor>
        <text evidence="10">Binds 1 copper ion per subunit, denoted as copper B.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>heme b</name>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
        <text evidence="10">Binds 2 heme groups per subunit, denoted as high- and low-spin.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3">Energy metabolism; oxidative phosphorylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 11">Belongs to the heme-copper respiratory oxidase family.</text>
    </comment>
    <dbReference type="EC" id="7.1.1.9" evidence="5"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08923.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008647464.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2044"/>
    <dbReference type="EnsemblBacteria" id="ABF08923">
        <property type="protein sequence ID" value="ABF08923"/>
        <property type="gene ID" value="Rmet_2044"/>
    </dbReference>
    <dbReference type="GeneID" id="60821377"/>
    <dbReference type="KEGG" id="rme:Rmet_2044"/>
    <dbReference type="eggNOG" id="COG3278">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017702_3_4_4"/>
    <dbReference type="OMA" id="WGWQLII"/>
    <dbReference type="OrthoDB" id="665754at2"/>
    <dbReference type="UniPathway" id="UPA00705"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045278">
        <property type="term" value="C:plasma membrane respiratory chain complex IV"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004129">
        <property type="term" value="F:cytochrome-c oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006119">
        <property type="term" value="P:oxidative phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd01661">
        <property type="entry name" value="cbb3_Oxidase_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.210.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023616">
        <property type="entry name" value="Cyt_c_oxase-like_su1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036927">
        <property type="entry name" value="Cyt_c_oxase-like_su1_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000883">
        <property type="entry name" value="Cyt_C_Oxase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023615">
        <property type="entry name" value="Cyt_c_Oxase_su1_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004677">
        <property type="entry name" value="Cyt_c_oxidase_cbb3_su1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10422">
        <property type="entry name" value="PTHR10422"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10422:SF29">
        <property type="entry name" value="PTHR10422:SF29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00115">
        <property type="entry name" value="COX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81442">
        <property type="entry name" value="SSF81442"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00780">
        <property type="entry name" value="ccoN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50855">
        <property type="entry name" value="COX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00077">
        <property type="entry name" value="COX1_CUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0249">Electron transport</keyword>
    <keyword evidence="10 11" id="KW-0349">Heme</keyword>
    <keyword evidence="10" id="KW-0408">Iron</keyword>
    <keyword evidence="8 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0679">Respiratory chain</keyword>
    <keyword evidence="6 11 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="11" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="21"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="65"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="97"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="132"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="161"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="207"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="241"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="278"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="310"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="353"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="387"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="438"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="domain" description="COX1" evidence="13">
        <location>
            <begin position="21"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="211"/>
        </location>
        <ligand>
            <name>Cu cation</name>
            <dbReference type="ChEBI" id="CHEBI:23378"/>
            <label>B</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="261"/>
        </location>
        <ligand>
            <name>Cu cation</name>
            <dbReference type="ChEBI" id="CHEBI:23378"/>
            <label>B</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="262"/>
        </location>
        <ligand>
            <name>Cu cation</name>
            <dbReference type="ChEBI" id="CHEBI:23378"/>
            <label>B</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009578"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00029368"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR604677-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50855"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ABF08923.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="482" mass="54186" checksum="0D1664B68D4C3C1D" modified="2006-05-30" version="1">MAVTTASSRADTFNYGVVRQFAVMTVVWGIVGMAVGVLLAAQLIWPDLNFNTPWLSFGRLRPLHTNAVIFAFGGSALFATSYYVVQRTCQARLFCGPLASFTFWGWQAIIIAAVITLPMGITSSKEYAELEWPIDILITLVWVAYAVVFFGTIIKRKTRHIYVANWFFGAYILTIAILHIVNNIEMPVSMWKSYSAYAGVQDAMIQWWYGHNAVGFFLTTSFLGMMYYFIPKQAERPIYSYRLSIVHFWALNFTYMWAGPHHLQYTSLPDWAQSLGMVFSLILLAPSWGGMINGIMTLSGAWHKLRTDPILKFLVVALSFYGMATFEGSMMSIKTVNALSHYTDWTIGHVHSGALGWVAMISIGSLYYLIPRLFGEKEMYSTRLIEWHFWIATIGVVLYIASMWIAGVMEGLMWRATQPDGTLTYAFVEAVKAKYPFYLIRLLGGLCFLSGMILMAYNVARTIIGKPAVDAPIPANLPASAH</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LM21</accession>
    <name>Q1LM21_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">MFS transporter, ACS family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">yfaV</name>
        <name evidence="7" type="ordered locus">Rmet_1926</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08805.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008651583.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1926"/>
    <dbReference type="EnsemblBacteria" id="ABF08805">
        <property type="protein sequence ID" value="ABF08805"/>
        <property type="gene ID" value="Rmet_1926"/>
    </dbReference>
    <dbReference type="GeneID" id="60821468"/>
    <dbReference type="KEGG" id="rme:Rmet_1926"/>
    <dbReference type="eggNOG" id="COG2271">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001265_0_0_4"/>
    <dbReference type="OMA" id="PPLWSMP"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="SSF103473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="31"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="69"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="101"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="125"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="161"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="194"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="260"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="294"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="328"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="352"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="383"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="417"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="domain" description="MFS" evidence="6">
        <location>
            <begin position="35"/>
            <end position="441"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABF08805.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="441" mass="47458" checksum="CA14494BCB6F2FDB" modified="2006-05-30" version="1">MSIVMSGAPGAPESLPAAELAQQRNEAYRKITVRLIPFLVFLFILAWIDRVNVGFAKLQMLQDLQFSEAVYGLGAGIFFIGYFLFEVPSNLLLEKIGARKTLARITILWGLASMAMIFVKTPMQFYTLRFVLGVFEAGFFPGVVLYLTYWFPAARRARINGLFMTSFAIAGVVGGPLAGFIMSAMDGVDGLANWQWLFVIEGIPSVLAGIAVLMYLPEKPVNAKWLTQQEQTIVTRDVEAEARDPNKHSSLKDAFANARVWICAAIYFCVVSGNATIAFWSPSIIKELGVQGNLQIGLVSAIPFVAGTIAMVLNGIHSDRTGERRMHCAMATLVAAVGLTLTGFLLHSAVLALIALTIASIGILAAFPVFWSLPSAFLAGTAAAGGIALINSIGNLAGFVAPYMIGWFKTSTGQLAGGLYFVAALEACATVLVIAFIRARH</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-02-05" modified="2022-08-03" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LM60</accession>
    <name>MNMC_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">tRNA 5-methylaminomethyl-2-thiouridine biosynthesis bifunctional protein MnmC</fullName>
            <shortName evidence="1">tRNA mnm(5)s(2)U biosynthesis bifunctional protein</shortName>
        </recommendedName>
        <domain>
            <recommendedName>
                <fullName evidence="1">tRNA (mnm(5)s(2)U34)-methyltransferase</fullName>
                <ecNumber evidence="1">2.1.1.61</ecNumber>
            </recommendedName>
        </domain>
        <domain>
            <recommendedName>
                <fullName evidence="1">FAD-dependent cmnm(5)s(2)U34 oxidoreductase</fullName>
                <ecNumber evidence="1">1.5.-.-</ecNumber>
            </recommendedName>
        </domain>
    </protein>
    <gene>
        <name evidence="1" type="primary">mnmC</name>
        <name type="ordered locus">Rmet_1887</name>
    </gene>
    <organism>
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the last two steps in the biosynthesis of 5-methylaminomethyl-2-thiouridine (mnm(5)s(2)U) at the wobble position (U34) in tRNA. Catalyzes the FAD-dependent demodification of cmnm(5)s(2)U34 to nm(5)s(2)U34, followed by the transfer of a methyl group from S-adenosyl-L-methionine to nm(5)s(2)U34, to form mnm(5)s(2)U34.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>5-aminomethyl-2-thiouridine(34) in tRNA + S-adenosyl-L-methionine = 5-methylaminomethyl-2-thiouridine(34) in tRNA + H(+) + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:19569"/>
            <dbReference type="Rhea" id="RHEA-COMP:10195"/>
            <dbReference type="Rhea" id="RHEA-COMP:10197"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:74454"/>
            <dbReference type="ChEBI" id="CHEBI:74455"/>
            <dbReference type="EC" id="2.1.1.61"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">In the N-terminal section; belongs to the methyltransferase superfamily. tRNA (mnm(5)s(2)U34)-methyltransferase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">In the C-terminal section; belongs to the DAO family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.61" evidence="1"/>
    <dbReference type="EC" id="1.5.-.-" evidence="1"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08766.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516612.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q1LM60"/>
    <dbReference type="SMR" id="Q1LM60"/>
    <dbReference type="STRING" id="266264.Rmet_1887"/>
    <dbReference type="EnsemblBacteria" id="ABF08766">
        <property type="protein sequence ID" value="ABF08766"/>
        <property type="gene ID" value="Rmet_1887"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1887"/>
    <dbReference type="eggNOG" id="COG0665">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG4121">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022427_1_0_4"/>
    <dbReference type="OMA" id="NFLCAWQ"/>
    <dbReference type="OrthoDB" id="912110at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016645">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-NH group of donors"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004808">
        <property type="term" value="F:tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002097">
        <property type="term" value="P:tRNA wobble base modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01102">
        <property type="entry name" value="MnmC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006076">
        <property type="entry name" value="FAD-dep_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008471">
        <property type="entry name" value="MnmC-like_methylTransf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023032">
        <property type="entry name" value="tRNA_MAMT_biosynth_bifunc_MnmC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017610">
        <property type="entry name" value="tRNA_S-uridine_synth_MnmC_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01266">
        <property type="entry name" value="DAO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05430">
        <property type="entry name" value="Methyltransf_30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="SSF51905"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03197">
        <property type="entry name" value="MnmC_Cterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0274">FAD</keyword>
    <keyword id="KW-0285">Flavoprotein</keyword>
    <keyword id="KW-0489">Methyltransferase</keyword>
    <keyword id="KW-0511">Multifunctional enzyme</keyword>
    <keyword id="KW-0560">Oxidoreductase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <keyword id="KW-0819">tRNA processing</keyword>
    <feature type="chain" id="PRO_1000065010" description="tRNA 5-methylaminomethyl-2-thiouridine biosynthesis bifunctional protein MnmC">
        <location>
            <begin position="1"/>
            <end position="672"/>
        </location>
    </feature>
    <feature type="region of interest" description="tRNA (mnm(5)s(2)U34)-methyltransferase">
        <location>
            <begin position="1"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="region of interest" description="FAD-dependent cmnm(5)s(2)U34 oxidoreductase">
        <location>
            <begin position="259"/>
            <end position="672"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01102"/>
        </source>
    </evidence>
    <sequence length="672" mass="71705" checksum="E28EDE6093144A2F" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="99" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LM69</accession>
    <name>Q1LM69_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Bifunctional NAD(P)H-hydrate repair enzyme</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">Nicotinamide nucleotide repair protein</fullName>
        </alternativeName>
        <domain>
            <recommendedName>
                <fullName evidence="16">ADP-dependent (S)-NAD(P)H-hydrate dehydratase</fullName>
                <ecNumber evidence="16">4.2.1.136</ecNumber>
            </recommendedName>
            <alternativeName>
                <fullName evidence="16">ADP-dependent NAD(P)HX dehydratase</fullName>
            </alternativeName>
        </domain>
        <domain>
            <recommendedName>
                <fullName evidence="16">NAD(P)H-hydrate epimerase</fullName>
                <ecNumber evidence="16">5.1.99.6</ecNumber>
            </recommendedName>
        </domain>
    </protein>
    <gene>
        <name evidence="14" type="primary">nnrD</name>
        <name evidence="15" type="synonym">nnrE</name>
        <name evidence="19" type="ordered locus">Rmet_1878</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13 16">Bifunctional enzyme that catalyzes the epimerization of the S- and R-forms of NAD(P)HX and the dehydration of the S-form of NAD(P)HX at the expense of ADP, which is converted to AMP. This allows the repair of both epimers of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration.</text>
    </comment>
    <comment type="function">
        <text evidence="14">Catalyzes the dehydration of the S-form of NAD(P)HX at the expense of ADP, which is converted to AMP. Together with NAD(P)HX epimerase, which catalyzes the epimerization of the S- and R-forms, the enzyme allows the repair of both epimers of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration.</text>
    </comment>
    <comment type="function">
        <text evidence="15">Catalyzes the epimerization of the S- and R-forms of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration. This is a prerequisite for the S-specific NAD(P)H-hydrate dehydratase to allow the repair of both epimers of NAD(P)HX.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 15 16">
            <text>(6R)-NADHX = (6S)-NADHX</text>
            <dbReference type="Rhea" id="RHEA:32215"/>
            <dbReference type="ChEBI" id="CHEBI:64074"/>
            <dbReference type="ChEBI" id="CHEBI:64075"/>
            <dbReference type="EC" id="5.1.99.6"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2 15 16">
            <text>(6R)-NADPHX = (6S)-NADPHX</text>
            <dbReference type="Rhea" id="RHEA:32227"/>
            <dbReference type="ChEBI" id="CHEBI:64076"/>
            <dbReference type="ChEBI" id="CHEBI:64077"/>
            <dbReference type="EC" id="5.1.99.6"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3 14 16">
            <text>(6S)-NADHX + ADP = AMP + H(+) + NADH + phosphate</text>
            <dbReference type="Rhea" id="RHEA:32223"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:64074"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="4.2.1.136"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4 14 16">
            <text>(6S)-NADPHX + ADP = AMP + H(+) + NADPH + phosphate</text>
            <dbReference type="Rhea" id="RHEA:32235"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:64076"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="4.2.1.136"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="15 16">
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </cofactor>
        <text evidence="15 16">Binds 1 potassium ion per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="14">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="14">Homotetramer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="14">Belongs to the NnrD/CARKD family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="15">Belongs to the NnrE/AIBP family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6 16">In the C-terminal section; belongs to the NnrD/CARKD family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5 16">In the N-terminal section; belongs to the NnrE/AIBP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.136" evidence="16"/>
    <dbReference type="EC" id="5.1.99.6" evidence="16"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08757.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516603.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1878"/>
    <dbReference type="EnsemblBacteria" id="ABF08757">
        <property type="protein sequence ID" value="ABF08757"/>
        <property type="gene ID" value="Rmet_1878"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1878"/>
    <dbReference type="eggNOG" id="COG0062">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG0063">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024853_4_3_4"/>
    <dbReference type="OMA" id="HNGGDAL"/>
    <dbReference type="OrthoDB" id="1748633at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052855">
        <property type="term" value="F:ADP-dependent NAD(P)H-hydrate dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052856">
        <property type="term" value="F:NADHX epimerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052857">
        <property type="term" value="F:NADPHX epimerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046496">
        <property type="term" value="P:nicotinamide nucleotide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01171">
        <property type="entry name" value="YXKO-related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1190.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10260">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01965">
        <property type="entry name" value="NADHX_dehydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01966">
        <property type="entry name" value="NADHX_epimerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017953">
        <property type="entry name" value="Carbohydrate_kinase_pred_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000631">
        <property type="entry name" value="CARKD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030677">
        <property type="entry name" value="Nnr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029056">
        <property type="entry name" value="Ribokinase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004443">
        <property type="entry name" value="YjeF_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036652">
        <property type="entry name" value="YjeF_N_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01256">
        <property type="entry name" value="Carb_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03853">
        <property type="entry name" value="YjeF_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017184">
        <property type="entry name" value="Nnr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53613">
        <property type="entry name" value="SSF53613"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64153">
        <property type="entry name" value="SSF64153"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00196">
        <property type="entry name" value="yjeF_cterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00197">
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    <feature type="domain" description="YjeF N-terminal" evidence="18">
        <location>
            <begin position="38"/>
            <end position="243"/>
        </location>
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        <location>
            <begin position="1"/>
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        </location>
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            <begin position="158"/>
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        </location>
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    <feature type="binding site" evidence="15">
        <location>
            <position position="84"/>
        </location>
        <ligand>
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </ligand>
    </feature>
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        <location>
            <position position="154"/>
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        <ligand>
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </ligand>
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        <location>
            <position position="187"/>
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            <dbReference type="PROSITE" id="PS51385"/>
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            <dbReference type="EMBL" id="ABF08757.1"/>
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    </evidence>
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            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="535" mass="54355" checksum="36066F3A327E7DEE" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LMD9</accession>
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            <fullName evidence="3">Phosphoenolpyruvate mutase</fullName>
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        <name evidence="5" type="primary">pepM</name>
        <name evidence="5" type="ordered locus">Rmet_1808</name>
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        <name type="synonym">Ralstonia metallidurans</name>
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            <taxon>Bacteria</taxon>
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            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
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    <reference evidence="6" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
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        <property type="project" value="UniProtKB-EC"/>
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        <property type="project" value="UniProtKB-KW"/>
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    <keyword evidence="5" id="KW-0670">Pyruvate</keyword>
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        <location>
            <begin position="307"/>
            <end position="424"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
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    </evidence>
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        <source>
            <dbReference type="EMBL" id="ABF08687.1"/>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
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    <sequence length="564" mass="61809" checksum="961498E65FADCB2E" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2011-04-05" modified="2022-08-03" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LMQ4</accession>
    <name>FLHC_CUPMC</name>
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        <recommendedName>
            <fullName evidence="1">Flagellar transcriptional regulator FlhC</fullName>
        </recommendedName>
    </protein>
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        <name evidence="1" type="primary">flhC</name>
        <name type="ordered locus">Rmet_1691</name>
    </gene>
    <organism>
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
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        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
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                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Functions in complex with FlhD as a master transcriptional regulator that regulates transcription of several flagellar and non-flagellar operons by binding to their promoter region. Activates expression of class 2 flagellar genes, including fliA, which is a flagellum-specific sigma factor that turns on the class 3 genes. Also regulates genes whose products function in a variety of physiological pathways.</text>
    </comment>
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            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="1">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Heterohexamer composed of two FlhC and four FlhD subunits. Each FlhC binds a FlhD dimer, forming a heterotrimer, and a hexamer assembles by dimerization of two heterotrimers.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the FlhC family.</text>
    </comment>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_007973.1"/>
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    <dbReference type="AlphaFoldDB" id="Q1LMQ4"/>
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    <dbReference type="EnsemblBacteria" id="ABF08572">
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    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1691"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044781">
        <property type="term" value="P:bacterial-type flagellum organization"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045893">
        <property type="term" value="P:positive regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902208">
        <property type="term" value="P:regulation of bacterial-type flagellum assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01891">
        <property type="entry name" value="FhlC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007944">
        <property type="entry name" value="FlhC"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05280">
        <property type="entry name" value="FlhC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF003159">
        <property type="entry name" value="FlhC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0010">Activator</keyword>
    <keyword id="KW-1005">Bacterial flagellum biogenesis</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0238">DNA-binding</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0804">Transcription</keyword>
    <keyword id="KW-0805">Transcription regulation</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <feature type="chain" id="PRO_0000406764" description="Flagellar transcriptional regulator FlhC">
        <location>
            <begin position="1"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="138"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="141"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="158"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="161"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01891"/>
        </source>
    </evidence>
    <sequence length="213" mass="24001" checksum="CECAC771F51C5F82" modified="2006-05-30" version="1">MTTRKSVLREVEEFRLAVELVELGARLQVLESEVAISRDRLTRLYKELRGVSPPKGQLPSSPEWFLTWRPNVHASLFLSAYRFLMAQGKQSRIRCIVSAYRLYVEHISLIGEDLTLSFTRAWTMLRFLDSGTLVSSTCKCCGGSFVRHKYDLQTNFVCGLCAPPPRAAKGKKLATSAEPFVVPEDLQQTISIEPAAMNRPKDAPLRRMGSLKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="62" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LMZ1</accession>
    <name>Q1LMZ1_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Glyceraldehyde 3-phosphate dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Rmet_1602</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell outer membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08485.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1602"/>
    <dbReference type="EnsemblBacteria" id="ABF08485">
        <property type="protein sequence ID" value="ABF08485"/>
        <property type="gene ID" value="Rmet_1602"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1602"/>
    <dbReference type="eggNOG" id="COG4775">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046092_2_0_4"/>
    <dbReference type="OMA" id="FHTWDGD"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009279">
        <property type="term" value="C:cell outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000184">
        <property type="entry name" value="Bac_surfAg_D15"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039910">
        <property type="entry name" value="D15-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12815">
        <property type="entry name" value="PTHR12815"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01103">
        <property type="entry name" value="Omp85"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1134">Transmembrane beta strand</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193636" evidence="5">
        <location>
            <begin position="39"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="domain" description="Omp85" evidence="6">
        <location>
            <begin position="255"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="34"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="38"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004442"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABF08485.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="425" mass="46831" checksum="4478030A545729DC" modified="2006-05-30" version="1">MDRLRRFQYDIWKMTRAILISALALLAAAAGSPVYGQAQPEDPSQSQTQTQTESTKPPESAKPADQMQPPAKKKLTFFDPEDGKLDMSDFLLNHKGALPVPTIITEPAVGYGFGLGLLFFSESMADAAAEARASGKGPAPPNITVLGGAYTDNGTWAAGFGHFHTWDGDRIRYLGGIGKVDANLEYFGVSNQPRSYELSGFALVQQLLIRVGDTHWFIGPRYVFADTSATFKFGNSAQELGSFERDQRIGKGGIVIDYDSRDNIFYPNKGSYAELEVQFARAGFGSTQTFDMYNARGFTWIPVARTLILGLRGDTRFSTGDVPFYAQPYVDLRGVQKGRYQDRNAVMTEVELRWDVTPRWSLLAFSGAGKAYGRWNDFSDASTVVSVGGGFRYMIARKLGLSMGIDVAHSRDQNAFYIQVGSAWH</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="98" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LN06</accession>
    <name>Q1LN06_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">2-methylisocitrate lyase</fullName>
            <shortName evidence="3">2-MIC</shortName>
            <shortName evidence="3">MICL</shortName>
            <ecNumber evidence="3">4.1.3.30</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">(2R,3S)-2-methylisocitrate lyase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="3 5" type="primary">prpB</name>
        <name evidence="5" type="ordered locus">Rmet_1587</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Catalyzes the thermodynamically favored C-C bond cleavage of (2R,3S)-2-methylisocitrate to yield pyruvate and succinate.</text>
    </comment>
    <comment type="function">
        <text evidence="3">Involved in the catabolism of short chain fatty acids (SCFA) via the 2-methylcitrate cycle (propionate degradation route). Catalyzes the thermodynamically favored C-C bond cleavage of (2R,3S)-2-methylisocitrate to yield pyruvate and succinate via an alpha-carboxy-carbanion intermediate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3 4">
            <text>(2S,3R)-3-hydroxybutane-1,2,3-tricarboxylate = pyruvate + succinate</text>
            <dbReference type="Rhea" id="RHEA:16809"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:57429"/>
            <dbReference type="EC" id="4.1.3.30"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 3">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3 4">Organic acid metabolism; propanoate degradation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homotetramer; dimer of dimers.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 3 4">Belongs to the isocitrate lyase/PEP mutase superfamily. Methylisocitrate lyase family.</text>
    </comment>
    <dbReference type="EC" id="4.1.3.30" evidence="3"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08470.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516322.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1587"/>
    <dbReference type="EnsemblBacteria" id="ABF08470">
        <property type="protein sequence ID" value="ABF08470"/>
        <property type="gene ID" value="Rmet_1587"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1587"/>
    <dbReference type="eggNOG" id="COG2513">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027389_3_2_4"/>
    <dbReference type="OMA" id="QTELWNK"/>
    <dbReference type="OrthoDB" id="1485205at2"/>
    <dbReference type="UniPathway" id="UPA00946"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046421">
        <property type="term" value="F:methylisocitrate lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019629">
        <property type="term" value="P:propionate catabolic process, 2-methylcitrate cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00377">
        <property type="entry name" value="ICL_PEPM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01939">
        <property type="entry name" value="PrpB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039556">
        <property type="entry name" value="ICL/PEPM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018523">
        <property type="entry name" value="Isocitrate_lyase_ph_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012695">
        <property type="entry name" value="PrpB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015813">
        <property type="entry name" value="Pyrv/PenolPyrv_Kinase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040442">
        <property type="entry name" value="Pyrv_Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51621">
        <property type="entry name" value="SSF51621"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02317">
        <property type="entry name" value="prpB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00161">
        <property type="entry name" value="ISOCITRATE_LYASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4 5" id="KW-0456">Lyase</keyword>
    <keyword evidence="3" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Substrate binding" evidence="3">
        <location>
            <begin position="51"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="3">
        <location>
            <begin position="129"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="3">
        <location>
            <begin position="216"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="91"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="93"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="164"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="194"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="247"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="276"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01939"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU361121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABF08470.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="302" mass="32755" checksum="6B5AED147287AC7E" modified="2006-05-30" version="1">MTFTATELARSAGARFRQALIDESPLQIPGTINANHALLAKRAGYRAIYLSGGGVAAGSLGLPDLGISNLDDVLTDIRRITDVCDTPLLVDVDTGFGASAFNVARTTRSLIKFGAAAMHIEDQVGAKRCGHRPNKEIVTKGEMVDRIRAAVDARTDENFVIMARTDALAVEGLDSAIERAVACVEAGADAIFPEAMTDMDMYRKFVDAVKVPVLANLTEFGATPYYTLDELRNAGVAMALYPLSAFRAMNKAAENVFQAIRREGTQKNVVDTMQTRAELYDRIGYHDFEQKLDALFAQGKGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="65" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LN31</accession>
    <name>Q1LN31_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_1562</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08445.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516299.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1562"/>
    <dbReference type="EnsemblBacteria" id="ABF08445">
        <property type="protein sequence ID" value="ABF08445"/>
        <property type="gene ID" value="Rmet_1562"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1562"/>
    <dbReference type="eggNOG" id="COG2253">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_058622_1_0_4"/>
    <dbReference type="OMA" id="RGFQTHA"/>
    <dbReference type="OrthoDB" id="798280at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014942">
        <property type="entry name" value="AbiEii"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08843">
        <property type="entry name" value="AbiEii"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF08445.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="306" mass="34441" checksum="92F9580D015C23A9" modified="2006-05-30" version="1">MNQTYLDTARLLTQVAPLVFVDDTFALKGGTAINLFVRDMPRLSVDLDLVFPDHTLPRDEALARINEAVRQAAERLKKRGFQTHAPAAAAGETKLLVRRGSIQVKVEVNFVMRGTVQPVRRASLTPVARDVLMADLEIPVVSLEDVYGGKLVAALDRQHPRDLFDVMQLFAHEGITPGIRRAFVVYLASSNRPIHEVLFTPLRDIRHDYEHNFQGMTVEPVPLDALLATRERMVREVQQGLDDDERRFLLSLVVGTPEWSLLGIAHLEHLPGIRWKLRNLAQLQKVDARKFGEQADLLAKQLSALC</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LP72</accession>
    <name>Q1LP72_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative acetyl transferase protein</fullName>
            <ecNumber evidence="2">2.3.1.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_1168</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.3.1.-" evidence="2"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08054.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515949.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1168"/>
    <dbReference type="EnsemblBacteria" id="ABF08054">
        <property type="protein sequence ID" value="ABF08054"/>
        <property type="gene ID" value="Rmet_1168"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1168"/>
    <dbReference type="eggNOG" id="COG0110">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_081811_1_1_4"/>
    <dbReference type="OMA" id="RHFHANL"/>
    <dbReference type="OrthoDB" id="1878940at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016746">
        <property type="term" value="F:acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03360">
        <property type="entry name" value="LbH_AT_putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001451">
        <property type="entry name" value="Hexapep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041561">
        <property type="entry name" value="PglD_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020019">
        <property type="entry name" value="Sia_OAcTrfase_NeuD-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011004">
        <property type="entry name" value="Trimer_LpxA-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00132">
        <property type="entry name" value="Hexapep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17836">
        <property type="entry name" value="PglD_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51161">
        <property type="entry name" value="SSF51161"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03570">
        <property type="entry name" value="NeuD_NnaD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PglD_N" evidence="1">
        <location>
            <begin position="2"/>
            <end position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF17836"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF08054.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="217" mass="22747" checksum="484B6A0B476746A3" modified="2006-05-30" version="1">MRIAIYGCSGFGREVAPIARDTARRIGSGTLNEAVVFVDDDVSNIGTIKNGIHVISFDELQSKQSDRLVVVAVGSSTVRRAIVAKCEAAGLKFASVSAPSARFLDEVSLGDGAIFCDFSMVTSNVRIGRHFHANIYSYVAHDCVVGDFVTLAPKACVNGNVILEDDVYVGTGAVIKQGTPEKPLRIGKGAIVGMGAVVTKDVPPGAVVVGNPARVKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LPC8</accession>
    <name>Q1LPC8_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Putative siderophore biosynthesis protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Rmet_1112</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the IucA/IucC family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07998.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515899.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1112"/>
    <dbReference type="EnsemblBacteria" id="ABF07998">
        <property type="protein sequence ID" value="ABF07998"/>
        <property type="gene ID" value="Rmet_1112"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1112"/>
    <dbReference type="eggNOG" id="COG4264">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018524_0_1_4"/>
    <dbReference type="OMA" id="HPWQWWN"/>
    <dbReference type="OrthoDB" id="856726at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016881">
        <property type="term" value="F:acid-amino acid ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019290">
        <property type="term" value="P:siderophore biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007310">
        <property type="entry name" value="Aerobactin_biosyn_IucA/IucC_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022770">
        <property type="entry name" value="FhuF_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037455">
        <property type="entry name" value="LucA/IucC-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34384">
        <property type="entry name" value="PTHR34384"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06276">
        <property type="entry name" value="FhuF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04183">
        <property type="entry name" value="IucA_IucC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="IucA_IucC" evidence="3">
        <location>
            <begin position="143"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="domain" description="FhuF" evidence="4">
        <location>
            <begin position="406"/>
            <end position="575"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="580"/>
            <end position="617"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="583"/>
            <end position="617"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF04183"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF06276"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABF07998.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="617" mass="67773" checksum="516FDBE4EC3F3E83" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LPU5</accession>
    <name>Q1LPU5_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Acyl-CoA dehydrogenase</fullName>
            <ecNumber evidence="8">1.3.8.1</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Rmet_0945</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the acyl-CoA dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.3.8.1" evidence="8"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07831.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515756.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0945"/>
    <dbReference type="EnsemblBacteria" id="ABF07831">
        <property type="protein sequence ID" value="ABF07831"/>
        <property type="gene ID" value="Rmet_0945"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0945"/>
    <dbReference type="eggNOG" id="COG1960">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018204_5_2_4"/>
    <dbReference type="OMA" id="DYRTIDN"/>
    <dbReference type="OrthoDB" id="760677at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004085">
        <property type="term" value="F:butyryl-CoA dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.540.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.110.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006091">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046373">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036250">
        <property type="entry name" value="AcylCo_DH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009075">
        <property type="entry name" value="AcylCo_DH/oxidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013786">
        <property type="entry name" value="AcylCoA_DH/ox_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037069">
        <property type="entry name" value="AcylCoA_DH/ox_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009100">
        <property type="entry name" value="AcylCoA_DH/oxidase_NM_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00441">
        <property type="entry name" value="Acyl-CoA_dh_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02770">
        <property type="entry name" value="Acyl-CoA_dh_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02771">
        <property type="entry name" value="Acyl-CoA_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47203">
        <property type="entry name" value="SSF47203"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56645">
        <property type="entry name" value="SSF56645"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0274">FAD</keyword>
    <keyword evidence="3" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Acyl-CoA_dh_N" evidence="7">
        <location>
            <begin position="6"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA_dh_M" evidence="6">
        <location>
            <begin position="119"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA_dh_1" evidence="5">
        <location>
            <begin position="216"/>
            <end position="348"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF02770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF02771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ABF07831.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="372" mass="39843" checksum="50E40ED1AE73341E" modified="2006-05-30" version="1">MNFNLSDEQKQLADAIRRFVEKSYDFESRKKNIKGANGYGEQAWGGLVELGLTALPVPEAQGGFSGTAVDMMVVQQELGRGLVVEPYFATVVAAQALKLAGGQEALLEQVAGGELKLATAFNEQQARHELNNVTVTAKGGKLNGRKVVVTHGAQADKLVVSARTSGAQSDRDGISLFLVDTKGAGVSVKDYRTIDNLHAADVTFQDAPATLIGTEGKGWDVLEATADYAAVLLCAEAVGVMDTLNAATLEYAKTRQQFGVAIARFQALQHRMVEMMIHAEQSRSITLLAAARFDEASPEERRRYVSAAKARVGVAARSVGQEAVQIHGGMGVTDELPAAHMFKRLTLINTTFGDVDHHLGRFASQPGFQEAA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2007-08-21" modified="2022-05-25" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LPV6</accession>
    <name>NUOH_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">NADH-quinone oxidoreductase subunit H</fullName>
            <ecNumber evidence="1">7.1.1.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">NADH dehydrogenase I subunit H</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">NDH-1 subunit H</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">nuoH</name>
        <name type="ordered locus">Rmet_0934</name>
    </gene>
    <organism>
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient. This subunit may bind ubiquinone.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a quinone + 5 H(+)(in) + NADH = a quinol + 4 H(+)(out) + NAD(+)</text>
            <dbReference type="Rhea" id="RHEA:57888"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:24646"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:132124"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">NDH-1 is composed of 14 different subunits. Subunits NuoA, H, J, K, L, M, N constitute the membrane sector of the complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the complex I subunit 1 family.</text>
    </comment>
    <dbReference type="EC" id="7.1.1.-" evidence="1"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07820.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008643351.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q1LPV6"/>
    <dbReference type="SMR" id="Q1LPV6"/>
    <dbReference type="STRING" id="266264.Rmet_0934"/>
    <dbReference type="EnsemblBacteria" id="ABF07820">
        <property type="protein sequence ID" value="ABF07820"/>
        <property type="gene ID" value="Rmet_0934"/>
    </dbReference>
    <dbReference type="GeneID" id="60825421"/>
    <dbReference type="KEGG" id="rme:Rmet_0934"/>
    <dbReference type="eggNOG" id="COG1005">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015134_0_1_4"/>
    <dbReference type="OMA" id="WSGWASN"/>
    <dbReference type="OrthoDB" id="1559067at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016655">
        <property type="term" value="F:oxidoreductase activity, acting on NAD(P)H, quinone or similar compound as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048038">
        <property type="term" value="F:quinone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01350">
        <property type="entry name" value="NDH1_NuoH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001694">
        <property type="entry name" value="NADH_UbQ_OxRdtase_su1/FPO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018086">
        <property type="entry name" value="NADH_UbQ_OxRdtase_su1_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11432">
        <property type="entry name" value="PTHR11432"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00146">
        <property type="entry name" value="NADHdh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00668">
        <property type="entry name" value="COMPLEX1_ND1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0997">Cell inner membrane</keyword>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-0520">NAD</keyword>
    <keyword id="KW-0874">Quinone</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-1278">Translocase</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <keyword id="KW-0830">Ubiquinone</keyword>
    <feature type="chain" id="PRO_0000298845" description="NADH-quinone oxidoreductase subunit H">
        <location>
            <begin position="1"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="22"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="91"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="124"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="162"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="203"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="250"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="291"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="326"/>
            <end position="346"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01350"/>
        </source>
    </evidence>
    <sequence length="354" mass="39832" checksum="050437429E602684" modified="2006-05-30" version="1">MIDWITSQGHDIFGMYWTPLWILIRAVLIVVPVLLCVAYLILWERKLIGWMHVRIGPNRVGPLGLLQPIADVLKLLLKEVMFPSQINRGMYLVAPLMVLMPAVAVWAVIPFQAEVVMADINAGLLYVMAISSVGVYGVILAGWASNSKYAFIGAMRAAAQMVSYEIAMGFALVTVLMVSGSLNLSHIVNAQNTGYFANMGLNLLSWNWLPLLPMFGVYFISGVAETNRHPFDVVEGESEIVAGHMIEYSGMTFALFFLAEYINMIIISTMTSLMFLGGWAPPFSSALTNAIPGFFWLVIKVFLLLSVFIWIRASFPRYRYDQIMRLGWKIFIPLTVAWLIIVAIWIKSPWNIWH</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LPX4</accession>
    <name>Q1LPX4_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">CDP-diacylglycerol--serine O-phosphatidyltransferase</fullName>
            <ecNumber evidence="4">2.7.8.8</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Phosphatidylserine synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">pssA</name>
        <name evidence="9" type="ordered locus">Rmet_0916</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a CDP-1,2-diacyl-sn-glycerol + L-serine = a 1,2-diacyl-sn-glycero-3-phospho-L-serine + CMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16913"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
            <dbReference type="ChEBI" id="CHEBI:57262"/>
            <dbReference type="ChEBI" id="CHEBI:58332"/>
            <dbReference type="ChEBI" id="CHEBI:60377"/>
            <dbReference type="EC" id="2.7.8.8"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 7">Belongs to the CDP-alcohol phosphatidyltransferase class-I family.</text>
    </comment>
    <dbReference type="EC" id="2.7.8.8" evidence="4"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07802.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008643331.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0916"/>
    <dbReference type="DNASU" id="4037712"/>
    <dbReference type="EnsemblBacteria" id="ABF07802">
        <property type="protein sequence ID" value="ABF07802"/>
        <property type="gene ID" value="Rmet_0916"/>
    </dbReference>
    <dbReference type="GeneID" id="60825403"/>
    <dbReference type="KEGG" id="rme:Rmet_0916"/>
    <dbReference type="eggNOG" id="COG1183">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_049944_2_1_4"/>
    <dbReference type="OMA" id="IFFDFFD"/>
    <dbReference type="OrthoDB" id="1232102at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003882">
        <property type="term" value="F:CDP-diacylglycerol-serine O-phosphatidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008654">
        <property type="term" value="P:phospholipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1760">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004533">
        <property type="entry name" value="CDP-diaglyc--ser_O-PTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000462">
        <property type="entry name" value="CDP-OH_P_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043130">
        <property type="entry name" value="CDP-OH_PTrfase_TM_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01066">
        <property type="entry name" value="CDP-OH_P_transf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00473">
        <property type="entry name" value="pssA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00379">
        <property type="entry name" value="CDP_ALCOHOL_P_TRANSF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 9" id="KW-0808">Transferase</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="50"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="139"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="169"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="202"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="230"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="255"/>
            <end position="274"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000287"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00017171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00032361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ABF07802.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="297" mass="32893" checksum="64C3217A6B8EC4A8" modified="2006-05-30" version="1">MGAFNRRNKRVNNGNVTHLRPFRRNQLRNDNAYDPDEHDLEYVRPRRRGIYLLPNAFTTAALFAGFFAIVQAMNMRFDVAAIAIFAAMVLDGMDGRVARITNTQSAFGEQYDSLSDMTSFGVAPALVMYEWILHDLGKWGWIAAFVYCTCAALRLARFNANIGVVDKRFFQGLPSPAAAALVAGFVWLAIDNKLPVKELWMPWVAFGITLYAGLSMVSNAPFYSGKALDVRYRVPFGMMVLVLVLFVVVSSDPPVALFGLFVAYAISGYLLWAWRTLHGQRGGVDKSRAPGNGSGGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LPY6</accession>
    <name>Q1LPY6_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Glycosyltransferase, group 1</fullName>
            <ecNumber evidence="3">2.4.1.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_0904</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.4.1.-" evidence="3"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07790.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515730.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0904"/>
    <dbReference type="CAZy" id="GT4">
        <property type="family name" value="Glycosyltransferase Family 4"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF07790">
        <property type="protein sequence ID" value="ABF07790"/>
        <property type="gene ID" value="Rmet_0904"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0904"/>
    <dbReference type="eggNOG" id="COG0438">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009583_2_0_4"/>
    <dbReference type="OMA" id="ERVDLWQ"/>
    <dbReference type="OrthoDB" id="948440at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028098">
        <property type="entry name" value="Glyco_trans_4-like_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13439">
        <property type="entry name" value="Glyco_transf_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glyco_trans_4-like_N" evidence="2">
        <location>
            <begin position="14"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="328"/>
            <end position="372"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13439"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF07790.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="372" mass="40612" checksum="E460444BF8A099DA" modified="2006-05-30" version="1">MRVLIVTDAWEPQVNGVVRTLKSTRRELEAMGHTVDLITPLEFQTIPCPTYPEIRLSLLPSGKVRRRIDTFRPDALHIATEGPLGLAARRYAMQHDLPFTTAYHTRFPEYVQARFGIPLAWTYRFLRWFHGSARAVMAPTPVVLKDLQDYGITNGVLWTRGVDLDVFTAQRANVLNTAHPIFLYVGRVAVEKNVEAFLQLDLPGSKWVVGDGPALAGLKARYPSANYLGVLKQPELAKVYASADVFVFPSRTDTFGLVLLEALASGLPVAAYPVTGPIDVLGNSPAGVMHEDLREACLEALRIDRATARAHAEKFSWRAASEQFLTHLRPLPTGTGGTDNSAPPVAPDHGQASSHATARSVAAPADGRSHGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="100" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LPZ6</accession>
    <name>Q1LPZ6_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Transcriptional regulator, LysR-family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_0894</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the LysR transcriptional regulatory family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07780.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515720.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0894"/>
    <dbReference type="EnsemblBacteria" id="ABF07780">
        <property type="protein sequence ID" value="ABF07780"/>
        <property type="gene ID" value="Rmet_0894"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0894"/>
    <dbReference type="eggNOG" id="COG0583">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039613_39_0_4"/>
    <dbReference type="OMA" id="WHERVHY"/>
    <dbReference type="OrthoDB" id="1591366at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005119">
        <property type="entry name" value="LysR_subst-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000847">
        <property type="entry name" value="Tscrpt_reg_HTH_LysR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00126">
        <property type="entry name" value="HTH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03466">
        <property type="entry name" value="LysR_substrate"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50931">
        <property type="entry name" value="HTH_LYSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH lysR-type" evidence="2">
        <location>
            <begin position="7"/>
            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF07780.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="316" mass="35883" checksum="296066B8D0E1F573" modified="2006-05-30" version="1">MHGRDHLDTYLLRVLHTLLTEQSVTRTAVRLGQSQPAISNTLKRLREITGDAILVRGKSGMVPTERGRELLLLAEQSLAAMDRIARPPQQFDPATTTRTFHLGAPDYLDAFFLPNIVERVRRLAPGAKLFVHPMTQSTDFLDALEQGHLDIVVGNWLSPPEHLHISPLFDDEVVCMLGAQHPLARHGLTLRHYLEMPHLAPAPYASMQRSMIDQALAEQGYKRNIQVTLPYFGLVPYVLMKTDMVFTTGRQFAAHYAQYLPIRMVPSPVQFPRMRFYQLWHERCHAAPDVMWMRRMIAEVATDLPQLPRLEATGAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LQ07</accession>
    <name>Q1LQ07_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">ygfU</name>
        <name evidence="8" type="ordered locus">Rmet_0883</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the nucleobase:cation symporter-2 (NCS2) (TC 2.A.40) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07769.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515709.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0883"/>
    <dbReference type="EnsemblBacteria" id="ABF07769">
        <property type="protein sequence ID" value="ABF07769"/>
        <property type="gene ID" value="Rmet_0883"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0883"/>
    <dbReference type="eggNOG" id="COG2233">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017959_8_2_4"/>
    <dbReference type="OMA" id="WANEMPL"/>
    <dbReference type="OrthoDB" id="1419334at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015205">
        <property type="term" value="F:nucleobase transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006043">
        <property type="entry name" value="NCS2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017588">
        <property type="entry name" value="UacT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006042">
        <property type="entry name" value="Xan_ur_permease"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00860">
        <property type="entry name" value="Xan_ur_permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00801">
        <property type="entry name" value="ncs2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03173">
        <property type="entry name" value="pbuX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01116">
        <property type="entry name" value="XANTH_URACIL_PERMASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="22"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="55"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="107"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="140"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="182"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="210"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="259"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="337"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="365"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="398"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="427"/>
            <end position="447"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ABF07769.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="468" mass="48768" checksum="E47934FAD7366129" modified="2006-05-30" version="1">MNSASTTVPTDLTNERLPSGRLLALGLQHVLVMYAGTVAVPLIVGGALKLPKDQLAFLINADLFAAGLATLIQAFGFWKFGIRMPVMMGVTFASVAPMIAIGNDPNVGLLGIYGAVIASGIFGILIAPMMGRMLGLFPPVVTGTVITLIGVSLMRVGINWAGGGQPTTRAVIDGVAKEVPNLAYGDLSNLAIAGLVLAVILLLSKYGRGLVANCAVLLGIIIGTLVAMALGKVSFEGLNEASYVAVITPLHFGIPKFELTAILSMCIVMLITLVESTGMFLALSDITGRKLSRDDLTAGLRADGLGTVIGGIFNTFPYTSFSQNVGLVTVTGVRSRYVAAAGGLILIAFGLFPKMAHVVASVPQFVLGGAGIVMFGMVAATGIRILGSCDFNRNRHNLFIVAISIGFGMIPTLSPTLFQYLPKWTDPFTHSGIVLGTIVAVALNLFFNGIQSAEEAMRNAAANSHGTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LQ09</accession>
    <name>Q1LQ09_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase</fullName>
            <ecNumber evidence="3">4.1.1.97</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Rmet_0881</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>5-hydroxy-2-oxo-4-ureido-2,5-dihydro-1H-imidazole-5-carboxylate + H(+) = (S)-allantoin + CO2</text>
            <dbReference type="Rhea" id="RHEA:26301"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15678"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:58639"/>
            <dbReference type="EC" id="4.1.1.97"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Purine metabolism; urate degradation; (S)-allantoin from urate: step 3/3.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.97" evidence="3"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07767.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515708.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0881"/>
    <dbReference type="EnsemblBacteria" id="ABF07767">
        <property type="protein sequence ID" value="ABF07767"/>
        <property type="gene ID" value="Rmet_0881"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0881"/>
    <dbReference type="eggNOG" id="COG3195">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_092522_1_1_4"/>
    <dbReference type="OMA" id="KICHLRL"/>
    <dbReference type="OrthoDB" id="1777613at2"/>
    <dbReference type="UniPathway" id="UPA00394">
        <property type="reaction ID" value="UER00652"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016831">
        <property type="term" value="F:carboxy-lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000255">
        <property type="term" value="P:allantoin metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006144">
        <property type="term" value="P:purine nucleobase metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019628">
        <property type="term" value="P:urate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3330.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018020">
        <property type="entry name" value="OHCU_decarboxylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017580">
        <property type="entry name" value="OHCU_decarboxylase-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036778">
        <property type="entry name" value="OHCU_decarboxylase_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09349">
        <property type="entry name" value="OHCU_decarbox"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF158694">
        <property type="entry name" value="SSF158694"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03164">
        <property type="entry name" value="UHCUDC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0210">Decarboxylase</keyword>
    <keyword evidence="6" id="KW-0456">Lyase</keyword>
    <keyword evidence="4" id="KW-0659">Purine metabolism</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="OHCU_decarbox" evidence="7">
        <location>
            <begin position="12"/>
            <end position="168"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004754"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022631"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF09349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ABF07767.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="173" mass="19072" checksum="B6BC277E96891AB8" modified="2006-05-30" version="1">MSQTTYTIAQLNTMPEAEFIAVLGGIYEHSPWFAETAARKRPFANTAELASALRGAVDAAGQEAQLKLVRAHPELAGKAAVRGELTAESTREQAGAGLDQCTPEEFQRLQGLNEAYNRKFGFPFILAVRGYDRHGIIDAFARRLENAPEVELQTCINQIHRIAQFRLNDLVSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="65" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LQN3</accession>
    <name>Q1LQN3_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Rmet_0657</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the glycosyltransferase 87 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07543.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515514.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0657"/>
    <dbReference type="EnsemblBacteria" id="ABF07543">
        <property type="protein sequence ID" value="ABF07543"/>
        <property type="gene ID" value="Rmet_0657"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0657"/>
    <dbReference type="eggNOG" id="ENOG502Z9HB">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037296_1_0_4"/>
    <dbReference type="OMA" id="LHIAGRM"/>
    <dbReference type="OrthoDB" id="1533723at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016758">
        <property type="term" value="F:hexosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018584">
        <property type="entry name" value="GT87"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09594">
        <property type="entry name" value="GT87"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="27"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="112"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="150"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="190"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="221"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="275"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="311"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="383"/>
            <end position="401"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00024033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ABF07543.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="408" mass="44014" checksum="826A38EAFE9D5B5B" modified="2006-05-30" version="1">MPAATDTPKTASLDSATPARWLTPGRICLYAATLLVLECVVVAAWWYGHSVIANPSVPRLGWDFVVYWCASGLAQMHGAVAAYDWELLRVAKTPLLPNTFGPFAYPPTFLLMIYPLAALPFSMALVLFALTGIALYLGYLRAVVGNLHRYWIVPALAFPGVWTTLISGQNSLYTLAAAGGALWLMRRHPVAAGACIALLCVKPQLGVLFPLMLLCERRWTAMAAAAGCSALFVGLTVMAFGVDIYPAFLHSLAMFRETVAVNIDTLRGAPTVFGVLYVAGASIPLANGVHAAVALCAIATCVWVWCTKPRLALSASALVVGTLLTQPYLIYYDLAWLAIPVALLCVDMIRFGSRGWERLLLIVTWFAPMQGLSPFFIDHVPQLAPLVLVCLLGLIVLRHQAARSAAQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="101" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LQT2</accession>
    <name>Q1LQT2_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Acetyl-CoA carboxylase, biotin carboxylase subunit</fullName>
            <ecNumber evidence="11">6.3.4.14</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">accC</name>
        <name evidence="11" type="ordered locus">Rmet_0608</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>biotin</name>
            <dbReference type="ChEBI" id="CHEBI:57586"/>
        </cofactor>
    </comment>
    <dbReference type="EC" id="6.3.4.14" evidence="11"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07494.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515467.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0608"/>
    <dbReference type="EnsemblBacteria" id="ABF07494">
        <property type="protein sequence ID" value="ABF07494"/>
        <property type="gene ID" value="Rmet_0608"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0608"/>
    <dbReference type="eggNOG" id="COG4770">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000395_3_1_4"/>
    <dbReference type="OMA" id="IYYDPML"/>
    <dbReference type="OrthoDB" id="361205at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004075">
        <property type="term" value="F:biotin carboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006164">
        <property type="term" value="P:purine nucleotide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011761">
        <property type="entry name" value="ATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005481">
        <property type="entry name" value="BC-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001882">
        <property type="entry name" value="Biotin_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011764">
        <property type="entry name" value="Biotin_carboxylation_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005482">
        <property type="entry name" value="Biotin_COase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000089">
        <property type="entry name" value="Biotin_lipoyl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005479">
        <property type="entry name" value="CbamoylP_synth_lsu-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016185">
        <property type="entry name" value="PreATP-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011054">
        <property type="entry name" value="Rudment_hybrid_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011053">
        <property type="entry name" value="Single_hybrid_motif"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02785">
        <property type="entry name" value="Biotin_carb_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00289">
        <property type="entry name" value="Biotin_carb_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00364">
        <property type="entry name" value="Biotin_lipoyl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02786">
        <property type="entry name" value="CPSase_L_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00878">
        <property type="entry name" value="Biotin_carb_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51230">
        <property type="entry name" value="SSF51230"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51246">
        <property type="entry name" value="SSF51246"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52440">
        <property type="entry name" value="SSF52440"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50975">
        <property type="entry name" value="ATP_GRASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50979">
        <property type="entry name" value="BC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00188">
        <property type="entry name" value="BIOTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50968">
        <property type="entry name" value="BIOTINYL_LIPOYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00866">
        <property type="entry name" value="CPSASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00867">
        <property type="entry name" value="CPSASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0092">Biotin</keyword>
    <keyword evidence="2 11" id="KW-0436">Ligase</keyword>
    <keyword evidence="3 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Biotin carboxylation" evidence="10">
        <location>
            <begin position="1"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="domain" description="ATP-grasp" evidence="9">
        <location>
            <begin position="120"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="domain" description="Lipoyl-binding" evidence="8">
        <location>
            <begin position="598"/>
            <end position="673"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ABF07494.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="679" mass="73327" checksum="794855DAAB741A7A" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="100" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LQY7</accession>
    <name>Q1LQY7_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">NAD-dependent formate dehydrogenase gamma subunit</fullName>
            <ecNumber evidence="7">1.2.1.2</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">fdsG</name>
        <name evidence="7" type="ordered locus">Rmet_0553</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>2Fe-2S cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49601"/>
        </cofactor>
        <text evidence="6">Binds 1 [2Fe-2S] cluster.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the complex I 24 kDa subunit family.</text>
    </comment>
    <dbReference type="EC" id="1.2.1.2" evidence="7"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07439.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515415.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0553"/>
    <dbReference type="EnsemblBacteria" id="ABF07439">
        <property type="protein sequence ID" value="ABF07439"/>
        <property type="gene ID" value="Rmet_0553"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0553"/>
    <dbReference type="eggNOG" id="COG1905">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_054362_2_2_4"/>
    <dbReference type="OMA" id="LRPVYCL"/>
    <dbReference type="OrthoDB" id="1206880at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.1590">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002023">
        <property type="entry name" value="NuoE-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041921">
        <property type="entry name" value="NuoE_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000216">
        <property type="entry name" value="NADH_DH_24kDa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="SSF52833"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01099">
        <property type="entry name" value="COMPLEX1_24K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 6" id="KW-0001">2Fe-2S</keyword>
    <keyword evidence="4 6" id="KW-0408">Iron</keyword>
    <keyword evidence="5 6" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="3 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="6">
        <location>
            <position position="88"/>
        </location>
        <ligand>
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="93"/>
        </location>
        <ligand>
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="129"/>
        </location>
        <ligand>
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="133"/>
        </location>
        <ligand>
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR000216-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABF07439.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="169" mass="18074" checksum="FF23DB635006803D" modified="2006-05-30" version="1">MPDTIPTGGDAAGVADAVARIVAARRDMPGALLPILHDIQDSQGFIPADAVSVVARALNLSRAEVHGVITFYHHFRERPAGRTVVQVCRAEACQSVGADALAAHARKALGCDFHETTADGQFTLEPVYCLGQCACGPAMTIGDRLHGRVDMQRFDKLIDAVREREEAQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LR15</accession>
    <name>Q1LR15_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">tRNA threonylcarbamoyladenosine biosynthesis protein TsaE</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">t(6)A37 threonylcarbamoyladenosine biosynthesis protein TsaE</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Rmet_0525</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TsaE family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07411.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515387.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0525"/>
    <dbReference type="EnsemblBacteria" id="ABF07411">
        <property type="protein sequence ID" value="ABF07411"/>
        <property type="gene ID" value="Rmet_0525"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0525"/>
    <dbReference type="eggNOG" id="COG0802">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_087829_2_0_4"/>
    <dbReference type="OMA" id="DAGFRDC"/>
    <dbReference type="OrthoDB" id="1827295at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002949">
        <property type="term" value="P:tRNA threonylcarbamoyladenosine modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003442">
        <property type="entry name" value="T6A_TsaE"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02367">
        <property type="entry name" value="TsaE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00150">
        <property type="entry name" value="T6A_YjeE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007599"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00019010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00032441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABF07411.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="177" mass="19422" checksum="67C3EB4E94370ADE" modified="2006-05-30" version="1">MPLLEERILPLPDEAATERFGAALATAARAMPPRTIHLQLSGDLGAGKTTLTRAVLRALGHVGKVRSPTYTLCEPYEVLRADGSPLTVYHFDLYRFADPEEWIDAGFRDCFAEPALNLVEWPEKAGRLLGEPDLHVLLQSDNRVSHWADNGADAGADRRMATLRAYTPTGLTLLNAC</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LRD6</accession>
    <name>Q1LRD6_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">D-malate dehydrogenase (decarboxylating)</fullName>
            <ecNumber evidence="5">1.1.1.83</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">ttuC</name>
        <name evidence="7" type="ordered locus">Rmet_0404</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(R)-malate + NAD(+) = CO2 + NADH + pyruvate</text>
            <dbReference type="Rhea" id="RHEA:18365"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15588"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.1.1.83"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the isocitrate and isopropylmalate dehydrogenases family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.83" evidence="5"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07290.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0404"/>
    <dbReference type="EnsemblBacteria" id="ABF07290">
        <property type="protein sequence ID" value="ABF07290"/>
        <property type="gene ID" value="Rmet_0404"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0404"/>
    <dbReference type="eggNOG" id="COG0473">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031953_0_1_4"/>
    <dbReference type="OMA" id="GTSMFEP"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046553">
        <property type="term" value="F:D-malate dehydrogenase (decarboxylating) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019818">
        <property type="entry name" value="IsoCit/isopropylmalate_DH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024084">
        <property type="entry name" value="IsoPropMal-DH-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011829">
        <property type="entry name" value="TTC_DH"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00180">
        <property type="entry name" value="Iso_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01329">
        <property type="entry name" value="Iso_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02089">
        <property type="entry name" value="TTC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00470">
        <property type="entry name" value="IDH_IMDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Iso_dh" evidence="6">
        <location>
            <begin position="1"/>
            <end position="328"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00013126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM01329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABF07290.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="332" mass="36529" checksum="1AFDE9F4DA03975B" modified="2006-05-30" version="1">MDATARRFGIDFQWDHFDFSSYAYYERHGKMLPDDWFDTLVKYDAIYFGAVGWPAKIADHVSLWGSLLQFRRSFDQYVNLRPVRLMPGIKSPLADRKPGDIDFYVVRENTEGEYSSIGGRMFPGTEREIVMQETVMSRHGVDRILKFAFELAQKRPKKHLTSATKSNGISITMPYWDERVEAMAANYPGMKVDKYHIDILTAHFVQHPDWFDVVVASNLFGDILSDLGPACTGTIGVAPSGNINPDKVYPSLFEPVHGSAPDIAGQGIANPIGQIWCGAMMLEHLGHAEAGAAVLAAIEQVLAAGPGHAPLTRDIGGTASTADLGQAIAEAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LRE4</accession>
    <name>Q1LRE4_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Putative cysteine dioxygenase type I</fullName>
            <ecNumber evidence="5">1.13.11.20</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Rmet_0396</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the cysteine dioxygenase family.</text>
    </comment>
    <dbReference type="EC" id="1.13.11.20" evidence="5"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07282.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515273.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0396"/>
    <dbReference type="EnsemblBacteria" id="ABF07282">
        <property type="protein sequence ID" value="ABF07282"/>
        <property type="gene ID" value="Rmet_0396"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0396"/>
    <dbReference type="eggNOG" id="COG5553">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_118472_0_0_4"/>
    <dbReference type="OMA" id="HAHHTWC"/>
    <dbReference type="OrthoDB" id="1571420at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017172">
        <property type="term" value="F:cysteine dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010300">
        <property type="entry name" value="CDO_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011051">
        <property type="entry name" value="RmlC_Cupin_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12918">
        <property type="entry name" value="PTHR12918"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05995">
        <property type="entry name" value="CDO_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51182">
        <property type="entry name" value="SSF51182"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="3" id="KW-0408">Iron</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0883">Thioether bond</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="95"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="97"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="148"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="cross-link" description="3'-(S-cysteinyl)-tyrosine (Cys-Tyr)" evidence="2">
        <location>
            <begin position="101"/>
            <end position="164"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR610300-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR610300-51"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABF07282.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="184" mass="19880" checksum="EDC3D0A952AB37EF" modified="2006-05-30" version="1">MQTSAPCASSGAPEPSPLTPFTSGIEATLAKNARMVAEQVAASLPDAETLLASLPPGAREGSPDTYTRHLVHADPFERFSVMLLVWRPGQFSPVHGHRTWCAYRVLRGTMYERHYRWDPESRTASQVGAVTREAGDTFSVPAGLQHIHALGNASDDVTVSLHIYGVEQSRISTGVNLLVDTVVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LRF1</accession>
    <name>Q1LRF1_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Enoyl-CoA hydratase/carnithine racemase</fullName>
            <ecNumber evidence="2">4.2.1.17</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_0389</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EC" id="4.2.1.17" evidence="2"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07275.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515266.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0389"/>
    <dbReference type="EnsemblBacteria" id="ABF07275">
        <property type="protein sequence ID" value="ABF07275"/>
        <property type="gene ID" value="Rmet_0389"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0389"/>
    <dbReference type="eggNOG" id="COG1024">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009834_7_2_4"/>
    <dbReference type="OMA" id="LGTHLNQ"/>
    <dbReference type="OrthoDB" id="1498685at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004300">
        <property type="term" value="F:enoyl-CoA hydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029045">
        <property type="entry name" value="ClpP/crotonase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001753">
        <property type="entry name" value="Enoyl-CoA_hydra/iso"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00378">
        <property type="entry name" value="ECH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52096">
        <property type="entry name" value="SSF52096"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2" id="KW-0456">Lyase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF07275.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="258" mass="27655" checksum="521E411869A573CE" modified="2006-05-30" version="1">MSIATSIDQGVLTIGFDRIDKKNAITAAMYQTMADALRAAETDAAVRVIVIEGKPEVFTAGNDIEDFLKRPPTNGADGAPAPVFQFLQAISRATKPIVASVSGAAVGIGTTLLLHCDLVYASETAKLALPFAQLGLCPEAASSLLLPRMVGYQRAAEKLLLGEAFSAQEAFEIGLVTKVLSVAELQGYVRQQAAKLAVLPASSLRETKRLMKGDDAAAVEKKMLEEGEVFRRMLVAPEAKEAFTAFFEKRKPDFSKFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="64" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LRT0</accession>
    <name>Q1LRT0_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_0260</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07146.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515153.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0260"/>
    <dbReference type="DNASU" id="4037046"/>
    <dbReference type="EnsemblBacteria" id="ABF07146">
        <property type="protein sequence ID" value="ABF07146"/>
        <property type="gene ID" value="Rmet_0260"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0260"/>
    <dbReference type="eggNOG" id="COG5488">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_096000_3_0_4"/>
    <dbReference type="OMA" id="FEFNPRW"/>
    <dbReference type="OrthoDB" id="1791802at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019253">
        <property type="entry name" value="DUF2244_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016990">
        <property type="entry name" value="UCP032162_TM"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10003">
        <property type="entry name" value="DUF2244"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF032162">
        <property type="entry name" value="UCP032162_imp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="43"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="70"/>
            <end position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF07146.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="171" mass="19071" checksum="B0D34FB70B2BE0B2" modified="2006-05-30" version="1">MQDLGIAFQTAVGDSGGNLDGRTPLPSNDWLMKRNCSLTPRQAGWFYFSIVCFSTAIAVFFAVWLGAWLVLPFAGLELLGLGIALLAYARHAADYERVSVTDGMLVVETASASRITREVFNPRWVRVELGESFRAPVVLRSDKRSVQVGLYLDPYRRRKFAQELAVALQRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="103" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LRU0</accession>
    <name>Q1LRU0_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">C-terminal processing peptidase</fullName>
            <ecNumber evidence="8">3.4.21.102</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">ctpA</name>
        <name evidence="8" type="ordered locus">Rmet_0250</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the peptidase S41A family.</text>
    </comment>
    <dbReference type="EC" id="3.4.21.102" evidence="8"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07136.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515145.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0250"/>
    <dbReference type="MEROPS" id="S41.004"/>
    <dbReference type="EnsemblBacteria" id="ABF07136">
        <property type="protein sequence ID" value="ABF07136"/>
        <property type="gene ID" value="Rmet_0250"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0250"/>
    <dbReference type="eggNOG" id="COG0793">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017295_1_1_4"/>
    <dbReference type="OMA" id="VICTRSI"/>
    <dbReference type="OrthoDB" id="1646508at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd07560">
        <property type="entry name" value="Peptidase_S41_CPP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029045">
        <property type="entry name" value="ClpP/crotonase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001478">
        <property type="entry name" value="PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004447">
        <property type="entry name" value="Peptidase_S41A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005151">
        <property type="entry name" value="Tail-specific_protease"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13180">
        <property type="entry name" value="PDZ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03572">
        <property type="entry name" value="Peptidase_S41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00228">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00245">
        <property type="entry name" value="TSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="SSF50156"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52096">
        <property type="entry name" value="SSF52096"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00225">
        <property type="entry name" value="prc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50106">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0645">Protease</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0720">Serine protease</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193792" evidence="6">
        <location>
            <begin position="30"/>
            <end position="535"/>
        </location>
    </feature>
    <feature type="domain" description="PDZ" evidence="7">
        <location>
            <begin position="88"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="402"/>
            <end position="535"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="402"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="477"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="507"/>
            <end position="525"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU004404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ABF07136.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="535" mass="58473" checksum="0F7D63B2409A3A8C" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="97" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LRV3</accession>
    <name>Q1LRV3_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Probable Mg chelatase-related protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_0237</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the Mg-chelatase subunits D/I family. ComM subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07123.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515136.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0237"/>
    <dbReference type="EnsemblBacteria" id="ABF07123">
        <property type="protein sequence ID" value="ABF07123"/>
        <property type="gene ID" value="Rmet_0237"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0237"/>
    <dbReference type="eggNOG" id="COG0606">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026145_1_1_4"/>
    <dbReference type="OMA" id="STNPCPC"/>
    <dbReference type="OrthoDB" id="581802at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032508">
        <property type="term" value="P:DNA duplex unwinding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045006">
        <property type="entry name" value="CHLI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001208">
        <property type="entry name" value="MCM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004482">
        <property type="entry name" value="Mg_chelat-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025158">
        <property type="entry name" value="Mg_chelat-rel_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000523">
        <property type="entry name" value="Mg_chelatse_chII-like_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32039">
        <property type="entry name" value="PTHR32039"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32039:SF7">
        <property type="entry name" value="PTHR32039:SF7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01078">
        <property type="entry name" value="Mg_chelatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13335">
        <property type="entry name" value="Mg_chelatase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01657">
        <property type="entry name" value="MCMFAMILY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="SSF54211"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00368">
        <property type="entry name" value="TIGR00368"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50051">
        <property type="entry name" value="MCM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MCM" evidence="2">
        <location>
            <begin position="301"/>
            <end position="396"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006354"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50051"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF07123.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="512" mass="55246" checksum="190BFE8C76C2B061" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-12-12" modified="2022-08-03" version="100" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LS04</accession>
    <name>GLMU_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Bifunctional protein GlmU</fullName>
        </recommendedName>
        <domain>
            <recommendedName>
                <fullName evidence="1">UDP-N-acetylglucosamine pyrophosphorylase</fullName>
                <ecNumber evidence="1">2.7.7.23</ecNumber>
            </recommendedName>
            <alternativeName>
                <fullName evidence="1">N-acetylglucosamine-1-phosphate uridyltransferase</fullName>
            </alternativeName>
        </domain>
        <domain>
            <recommendedName>
                <fullName evidence="1">Glucosamine-1-phosphate N-acetyltransferase</fullName>
                <ecNumber evidence="1">2.3.1.157</ecNumber>
            </recommendedName>
        </domain>
    </protein>
    <gene>
        <name evidence="1" type="primary">glmU</name>
        <name type="ordered locus">Rmet_0186</name>
    </gene>
    <organism>
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the last two sequential reactions in the de novo biosynthetic pathway for UDP-N-acetylglucosamine (UDP-GlcNAc). The C-terminal domain catalyzes the transfer of acetyl group from acetyl coenzyme A to glucosamine-1-phosphate (GlcN-1-P) to produce N-acetylglucosamine-1-phosphate (GlcNAc-1-P), which is converted into UDP-GlcNAc by the transfer of uridine 5-monophosphate (from uridine 5-triphosphate), a reaction catalyzed by the N-terminal domain.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>acetyl-CoA + alpha-D-glucosamine 1-phosphate = CoA + H(+) + N-acetyl-alpha-D-glucosamine 1-phosphate</text>
            <dbReference type="Rhea" id="RHEA:13725"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:57776"/>
            <dbReference type="ChEBI" id="CHEBI:58516"/>
            <dbReference type="EC" id="2.3.1.157"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>H(+) + N-acetyl-alpha-D-glucosamine 1-phosphate + UTP = diphosphate + UDP-N-acetyl-alpha-D-glucosamine</text>
            <dbReference type="Rhea" id="RHEA:13509"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:46398"/>
            <dbReference type="ChEBI" id="CHEBI:57705"/>
            <dbReference type="ChEBI" id="CHEBI:57776"/>
            <dbReference type="EC" id="2.7.7.23"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="1">Binds 1 Mg(2+) ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Nucleotide-sugar biosynthesis; UDP-N-acetyl-alpha-D-glucosamine biosynthesis; N-acetyl-alpha-D-glucosamine 1-phosphate from alpha-D-glucosamine 6-phosphate (route II): step 2/2.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Nucleotide-sugar biosynthesis; UDP-N-acetyl-alpha-D-glucosamine biosynthesis; UDP-N-acetyl-alpha-D-glucosamine from N-acetyl-alpha-D-glucosamine 1-phosphate: step 1/1.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Bacterial outer membrane biogenesis; LPS lipid A biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homotrimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">In the N-terminal section; belongs to the N-acetylglucosamine-1-phosphate uridyltransferase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">In the C-terminal section; belongs to the transferase hexapeptide repeat family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.23" evidence="1"/>
    <dbReference type="EC" id="2.3.1.157" evidence="1"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07072.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008641833.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q1LS04"/>
    <dbReference type="SMR" id="Q1LS04"/>
    <dbReference type="STRING" id="266264.Rmet_0186"/>
    <dbReference type="PRIDE" id="Q1LS04"/>
    <dbReference type="EnsemblBacteria" id="ABF07072">
        <property type="protein sequence ID" value="ABF07072"/>
        <property type="gene ID" value="Rmet_0186"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0186"/>
    <dbReference type="eggNOG" id="COG1207">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029499_15_2_4"/>
    <dbReference type="OMA" id="TAIVEHK"/>
    <dbReference type="OrthoDB" id="1381953at2"/>
    <dbReference type="UniPathway" id="UPA00113">
        <property type="reaction ID" value="UER00532"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00113">
        <property type="reaction ID" value="UER00533"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00973"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
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        <property type="term" value="F:UDP-N-acetylglucosamine diphosphorylase activity"/>
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        <property type="term" value="P:lipid A biosynthetic process"/>
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        <property type="term" value="P:peptidoglycan biosynthetic process"/>
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        <property type="project" value="UniProtKB-UniPathway"/>
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        <property type="entry name" value="LbH_GlmU_C"/>
        <property type="match status" value="1"/>
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    </dbReference>
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        <property type="entry name" value="Hexapep"/>
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    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0012">Acyltransferase</keyword>
    <keyword id="KW-0133">Cell shape</keyword>
    <keyword id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0511">Multifunctional enzyme</keyword>
    <keyword id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword id="KW-0573">Peptidoglycan synthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
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    <keyword id="KW-0808">Transferase</keyword>
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        <location>
            <begin position="1"/>
            <end position="454"/>
        </location>
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        <location>
            <begin position="1"/>
            <end position="225"/>
        </location>
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        <location>
            <begin position="226"/>
            <end position="246"/>
        </location>
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            <end position="454"/>
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        <location>
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        <location>
            <begin position="6"/>
            <end position="9"/>
        </location>
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            <name>UDP-N-acetyl-alpha-D-glucosamine</name>
            <dbReference type="ChEBI" id="CHEBI:57705"/>
        </ligand>
    </feature>
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        <location>
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        </ligand>
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        <location>
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        </ligand>
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    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01631"/>
        </source>
    </evidence>
    <sequence length="454" mass="48654" checksum="8FB35B29F66E8049" modified="2006-05-30" version="1">MNIVILAAGMGKRMNSALPKVLHPVAGQPMLSHVLDTARTLSPSRLVVVVGHGAELVRKAVGADDVAFAEQAQQLGTGHAVMQALPLLDDSQPTLVLYGDVPLTSVDTLKGLVQAAGAERLGVLTVEMPDPTGYGRIVRDAAGNIVRIVEQKDASEDVRAIREINTGIIVCPTAHLRQWLATLRNDNSQGEYYLTDTIERAVNDGVEVVSAQPAALWETLGVNSKVQLAEIERIHQRNIAQRLLEAGVTLLDPARIDVRGELTCGRDVTIDVGCVFEGRVHLEDGVSVGAHCVVRNTTIGAGARIQPFCHFEDAKVGPDGRIGPYARLRPGTELGQDVHIGNFVEIKNSQIADHSKANHLAYVGDATVGQRVNIGAGTITCNYDGVNKHRTVLEDDVFIGSDTQLVAPVTVRRGATIGAGTTLTKEAPADKLTLSRAKQMTLDAWQRPVKQPKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LS68</accession>
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        <submittedName>
            <fullName evidence="2">Putative acetyltransferase</fullName>
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        <name evidence="2" type="ordered locus">Rmet_0122</name>
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    </organism>
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        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
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                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
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        <property type="term" value="F:N-acetyltransferase activity"/>
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    </dbReference>
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    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="1">
        <location>
            <begin position="9"/>
            <end position="168"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF07008.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="183" mass="20058" checksum="5424B720A460399E" modified="2006-05-30" version="1">MQPLRGKDFLLRPFRASDVDPFVAAVRESMGTVGAWMPWAHPDYNTLDAQEWFARCETCMEEGSAYDVGVFSADGRTLYGGVAINQIRREDNLGNLGYWIRQSHQRQGLASGAATMMACHGFHALGLTRIEIVAAETNLASRGVAEKIGAAFECIARNRLILGGRPVAAAVYSLVPESFDFSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-03-23" modified="2020-10-07" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>D3DXW1</accession>
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        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name evidence="1" type="ordered locus">Rmet_6524</name>
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        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
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        <lineage>
            <taxon>Bacteria</taxon>
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        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ADC45131.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_6524"/>
    <dbReference type="EnsemblBacteria" id="ADC45131">
        <property type="protein sequence ID" value="ADC45131"/>
        <property type="gene ID" value="Rmet_6524"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6524"/>
    <dbReference type="HOGENOM" id="CLU_2976089_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADC45131.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="58" mass="6273" checksum="D6717BCA4BEB902A" modified="2010-03-23" version="1">MVHLTFWEAPARSWVDLSGAGAKKLGRTELAPIRDFTGFSRTSVRELNSGDAGRGAPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-03-23" modified="2021-06-02" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>D3DY01</accession>
    <name>D3DY01_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_6567</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ADC45171.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_6567"/>
    <dbReference type="EnsemblBacteria" id="ADC45171">
        <property type="protein sequence ID" value="ADC45171"/>
        <property type="gene ID" value="Rmet_6567"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6567"/>
    <dbReference type="HOGENOM" id="CLU_2957380_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="39"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="43"/>
            <end position="59"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADC45171.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="59" mass="6523" checksum="8F3F86A351775B95" modified="2010-03-23" version="1">MDVAHDASDHALRSRLNPHRGPVRTLLRVARRLFANRSGRSGARCARLSTTPTAMTGQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-03-23" modified="2020-10-07" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>D3DY26</accession>
    <name>D3DY26_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_6595</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ADC45196.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_6595"/>
    <dbReference type="EnsemblBacteria" id="ADC45196">
        <property type="protein sequence ID" value="ADC45196"/>
        <property type="gene ID" value="Rmet_6595"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6595"/>
    <dbReference type="HOGENOM" id="CLU_2344602_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADC45196.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="97" mass="10803" checksum="F3883919FAC7062F" modified="2010-03-23" version="1">MDNRLISCRINGLRLPEPLGQAGYVYVDTGGQVFFPPAESGKLAELFVYVPHATPSRTTGCSPGCPQVNRPFVHRPRMVRDHRQGCCRWMPLPCSCT</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-03-23" modified="2020-10-07" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>D3DY48</accession>
    <name>D3DY48_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_6619</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ADC45218.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_6619"/>
    <dbReference type="EnsemblBacteria" id="ADC45218">
        <property type="protein sequence ID" value="ADC45218"/>
        <property type="gene ID" value="Rmet_6619"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6619"/>
    <dbReference type="HOGENOM" id="CLU_2651867_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADC45218.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="76" mass="8484" checksum="48DB53A9E5823ED6" modified="2010-03-23" version="1">MVDGKAGNGNGYNARNARGLMLDTQDVISYFDSLYCVRMAEHMQPMGSARVIIGDASTQWRVNDGQKRMRCNANVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-03-23" modified="2020-10-07" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>D3DY54</accession>
    <name>D3DY54_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_3761</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ADC45224.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3761"/>
    <dbReference type="EnsemblBacteria" id="ADC45224">
        <property type="protein sequence ID" value="ADC45224"/>
        <property type="gene ID" value="Rmet_3761"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3761"/>
    <dbReference type="eggNOG" id="COG3316">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2668426_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADC45224.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="75" mass="8774" checksum="B06CE3529CA26BE5" modified="2010-03-23" version="1">MSLRDIEELLFERGVVVSYETTRRWCDKFGGNFSHRVTAVRRRPGSTWHLDERPRKTLGFDTPAERFHQPVALTG</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-03-23" modified="2021-06-02" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>D3DYI2</accession>
    <name>D3DYI2_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_6357</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="1 2">
        <name>pMOL30</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid pMOL30</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000354">
        <property type="protein sequence ID" value="ADC45352.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011514790.1">
        <property type="nucleotide sequence ID" value="NC_007971.2"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADC45352">
        <property type="protein sequence ID" value="ADC45352"/>
        <property type="gene ID" value="Rmet_6357"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6357"/>
    <dbReference type="HOGENOM" id="CLU_2685194_0_0_4"/>
    <dbReference type="OrthoDB" id="2209357at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid pMOL30"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADC45352.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="74" mass="8074" checksum="7B1B843D91220FCF" modified="2010-03-23" version="1">MSMILTEAERVAIRGLASGDKTQFEAAQGAFNRAARQHGVDSCVELQFMAELLAPVPDLLLRSQYRAAVLKQAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-03-23" modified="2021-06-02" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>D3DYI8</accession>
    <name>D3DYI8_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_6380</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3 4">
        <name>pMOL30</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid pMOL30</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000354">
        <property type="protein sequence ID" value="ADC45358.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADC45358">
        <property type="protein sequence ID" value="ADC45358"/>
        <property type="gene ID" value="Rmet_6380"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6380"/>
    <dbReference type="HOGENOM" id="CLU_2900992_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid pMOL30"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="12"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="41"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="42"/>
            <end position="62"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADC45358.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="62" mass="7245" checksum="FBDF246B457644C6" modified="2010-03-23" version="1">MNRYYVFDLPHGVLSSLLLVAVLCIIAFLAFVIYLNRTYGRGRPRLPRRGRPRKSPRSGRRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="68" xmlns="http://uniprot.org/uniprot">
    <accession>Q1L9P0</accession>
    <name>Q1L9P0_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Primase (TraC1)(Plasmid backbone)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">pri</name>
        <name evidence="8" type="ordered locus">Rmet_6277</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="8 9">
        <name>pMOL28</name>
    </geneLocation>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="9">Plasmid pMOL28</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000355">
        <property type="protein sequence ID" value="ABF13136.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011514906.1">
        <property type="nucleotide sequence ID" value="NC_007972.2"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF13136">
        <property type="protein sequence ID" value="ABF13136"/>
        <property type="gene ID" value="Rmet_6277"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6277"/>
    <dbReference type="HOGENOM" id="CLU_059689_1_0_4"/>
    <dbReference type="OMA" id="KSPGMAD"/>
    <dbReference type="OrthoDB" id="1071997at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid pMOL28"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000428">
        <property type="term" value="C:DNA-directed RNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990077">
        <property type="term" value="C:primosome complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003896">
        <property type="term" value="F:DNA primase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.580.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036977">
        <property type="entry name" value="DNA_primase_Znf_CHC2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013237">
        <property type="entry name" value="Phage_T7_Gp4_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006171">
        <property type="entry name" value="TOPRIM_domain"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08273">
        <property type="entry name" value="Prim_Zn_Ribbon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13362">
        <property type="entry name" value="Toprim_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00778">
        <property type="entry name" value="Prim_Zn_Ribbon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0235">DNA replication</keyword>
    <keyword evidence="1" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="4" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="8" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-0639">Primosome</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Prim_Zn_Ribbon" evidence="7">
        <location>
            <begin position="36"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00778"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ABF13136.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="355" mass="39720" checksum="9112F71EE6346566" modified="2006-05-30" version="1">MQDISVIVKSWKAEQWDALIAQYLPAGILTDAFWRGRPGPCPICGGKDRFSYTRNRGRGDWVCRKCNNGSPAGGDGLELIRRYTNMSYSELKRRLNGQCSEPLPPVAARVPAREVVPPSELMRRVMKQVSKSKPVQPGDAAMRYLAARVPGLRAPIPQSLRMAEQDYWHERKVVGKFPTMIAEYRLADGRLATVHRTSLHPDKPEKAIVVSDDGEILPSKRNYTGALPLDGGAVRLMAPRHGEIGVAEGIENAYAAYMLFRVPTWSCLNRVLMSKFVVPNDLGIHTVHIFADFDKVDAKTGKSPGMADALTLQKRLRREGFNVVLHRPKVRGSDFCDEWRTVYQLREISQQAVAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="62" xmlns="http://uniprot.org/uniprot">
    <accession>Q1L9Q5</accession>
    <name>Q1L9Q5_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Alpha/beta hydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_6262</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2 3">
        <name>pMOL28</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid pMOL28</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000355">
        <property type="protein sequence ID" value="ABF13121.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF13121">
        <property type="protein sequence ID" value="ABF13121"/>
        <property type="gene ID" value="Rmet_6262"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6262"/>
    <dbReference type="HOGENOM" id="CLU_020336_29_1_4"/>
    <dbReference type="OMA" id="HPFDRTM"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid pMOL28"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12697">
        <property type="entry name" value="Abhydrolase_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="SSF53474"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AB hydrolase-1" evidence="1">
        <location>
            <begin position="42"/>
            <end position="211"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF13121.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="230" mass="25187" checksum="B9765DD4E7296EE0" modified="2006-05-30" version="1">MLPLILLPAMPCDERMYAAQVSGLSDLTECRVMVVDAQSFDAGAEHLLTSIHGRFIIAGTAYGGCLAIETAIRAPERVAGLWLMNCNPGAHPDLDAVRETSFRLRSGQLDQVLEEFATAAMPDTAADCRTVFIEMGRVIGAEVFARQSDAALTRRDHWDKLSSIVAPTLITWGMVDRFLPTGIGEEMARLMPHAQLDMLEGCGHFPSLEQPLLTTALARNWLLKHQNALT</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LA60</accession>
    <name>Q1LA60_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">CopM involved in Cu(II)/Cu(I) resistance</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">copM</name>
        <name evidence="2" type="ordered locus">Rmet_6107</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2 3">
        <name>pMOL30</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid pMOL30</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000354">
        <property type="protein sequence ID" value="ABF12966.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF12966">
        <property type="protein sequence ID" value="ABF12966"/>
        <property type="gene ID" value="Rmet_6107"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6107"/>
    <dbReference type="HOGENOM" id="CLU_1873724_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid pMOL30"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="78"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="89"/>
            <end position="109"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF12966.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="136" mass="14466" checksum="ACFB9E2AAEF747FE" modified="2006-05-30" version="1">MKRQFSNAPSATCNVRMRMKAGMPLLLSVGAAICLVPGQVWAIQLAWVAETPTGLGIDGIKAERRDDGDEVQLLVLAANPRHHSMKHVTPPKATSQPPDRSTPASAPDSQQAGCEDDKKTRSTEPVVPQHGGPNAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="56" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LAE3</accession>
    <name>Q1LAE3_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative lipoprotein transmembrane</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_6024</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3 4">
        <name>pMOL30</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid pMOL30</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000354">
        <property type="protein sequence ID" value="ABF12883.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF12883">
        <property type="protein sequence ID" value="ABF12883"/>
        <property type="gene ID" value="Rmet_6024"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6024"/>
    <dbReference type="HOGENOM" id="CLU_080659_0_0_4"/>
    <dbReference type="OMA" id="ACTYEAS"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid pMOL30"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193237" evidence="2">
        <location>
            <begin position="33"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="37"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="54"/>
            <end position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF12883.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="323" mass="31801" checksum="2361D9217D76061F" modified="2006-05-30" version="1">MPNSTVQGDVLMMKSTRSFAILAGLAASLALAACGGGGEDAVKPDSAPIISTPQPGNPSNPTPAEPAQPAGPTTPSNPSGPTTPGLTVTGSASLVGSQNVTALFAGTALKPTGLAGNFLSALTVPATAQAVTAEGAYTTLGASNGAMSVKLSDVAEIDDVAGNGQYAIGRWKVGSFDVTTPTVNNTQTLRANQAAPYAVGIPLDLYSGPSTGTLACTYEASTKPTSLDGSIAAGTFNGAGSSAIINLATKVITLNLSLDIGADHGVTVSKANILVDMPTTGGGSSVIAETVGSDLSKPYLVIAYGTVTPTSGDVGGLAVFRCQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LAU8</accession>
    <name>Q1LAU8_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Extra-cytoplasmic solute receptor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">bug</name>
        <name evidence="4" type="ordered locus">Rmet_5869</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="5">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="5">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Periplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the UPF0065 (bug) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12728.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518078.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5869"/>
    <dbReference type="EnsemblBacteria" id="ABF12728">
        <property type="protein sequence ID" value="ABF12728"/>
        <property type="gene ID" value="Rmet_5869"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5869"/>
    <dbReference type="eggNOG" id="COG3181">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045683_0_0_4"/>
    <dbReference type="OMA" id="AGDMFKM"/>
    <dbReference type="OrthoDB" id="447734at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042597">
        <property type="term" value="C:periplasmic space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005064">
        <property type="entry name" value="BUG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042100">
        <property type="entry name" value="Bug_dom1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42928">
        <property type="entry name" value="PTHR42928"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03401">
        <property type="entry name" value="TctC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017082">
        <property type="entry name" value="YflP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-0675">Receptor</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193021" evidence="3">
        <location>
            <begin position="46"/>
            <end position="346"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF12728.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="346" mass="36160" checksum="C40AC4458E57C6BF" modified="2006-05-30" version="1">MPVVRPGEHQPACKSALQSASSQRRASCAALLALAAWCIAPSAQAAQAAIAGGKPIRILVGAPAGGTTDTLARTIAQEMSQTLDQPVVVENRPGAGGNIAADMVAKSPADGTTLLMSFTSHTINATLYKKLPFDPIQDFTPITLVATVPSVLVARPNLPANNIPELIRLAKSEPGKLNFGIGAVGSSVHLAGDMFKMMTGTYIVNIPYKGTTPAITDLLAGQVDLMFASTINVAQHVKAGKLKVLGVTSPTPLPQFPGAQPIGATVKGFESSAWFGLFGPANLPPEITQALYQAARHGLEQPAVRKRLETDGAQPSAMPPEAFANFVKQDVKRWATVVKYSGAKPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="92" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LAV8</accession>
    <name>Q1LAV8_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">GDP-L-fucose synthase</fullName>
            <ecNumber evidence="2">1.1.1.271</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">GDP-4-keto-6-deoxy-D-mannose-3,5-epimerase-4-reductase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="4" type="primary">wcaG</name>
        <name evidence="2" type="synonym">fcl</name>
        <name evidence="4" type="ordered locus">Rmet_5859</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="5">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="5">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Catalyzes the two-step NADP-dependent conversion of GDP-4-dehydro-6-deoxy-D-mannose to GDP-fucose, involving an epimerase and a reductase reaction.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>GDP-beta-L-fucose + NADP(+) = GDP-4-dehydro-alpha-D-rhamnose + H(+) + NADPH</text>
            <dbReference type="Rhea" id="RHEA:18885"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57273"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:57964"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.1.1.271"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Nucleotide-sugar biosynthesis; GDP-L-fucose biosynthesis via de novo pathway; GDP-L-fucose from GDP-alpha-D-mannose: step 2/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the NAD(P)-dependent epimerase/dehydratase family. Fucose synthase subfamily.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.271" evidence="2"/>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12718.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5859"/>
    <dbReference type="EnsemblBacteria" id="ABF12718">
        <property type="protein sequence ID" value="ABF12718"/>
        <property type="gene ID" value="Rmet_5859"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5859"/>
    <dbReference type="eggNOG" id="COG0451">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007383_18_0_4"/>
    <dbReference type="OMA" id="IHCAGRV"/>
    <dbReference type="OrthoDB" id="1558163at2"/>
    <dbReference type="UniPathway" id="UPA00128">
        <property type="reaction ID" value="UER00191"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050577">
        <property type="term" value="F:GDP-L-fucose synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016853">
        <property type="term" value="F:isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070401">
        <property type="term" value="F:NADP+ binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042351">
        <property type="term" value="P:'de novo' GDP-L-fucose biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd05239">
        <property type="entry name" value="GDP_FS_SDR_e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00956">
        <property type="entry name" value="GDP_fucose_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001509">
        <property type="entry name" value="Epimerase_deHydtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028614">
        <property type="entry name" value="GDP_fucose/colitose_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01370">
        <property type="entry name" value="Epimerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0413">Isomerase</keyword>
    <keyword evidence="2" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="2" id="KW-0521">NADP</keyword>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-0614">Plasmid</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Epimerase" evidence="3">
        <location>
            <begin position="11"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="2">
        <location>
            <position position="141"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="15"/>
            <end position="21"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="110"/>
            <end position="113"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="145"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="168"/>
            <end position="171"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="184"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="192"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="207"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="214"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="282"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Important for catalytic activity" evidence="2">
        <location>
            <position position="112"/>
        </location>
    </feature>
    <feature type="site" description="Important for catalytic activity" evidence="2">
        <location>
            <position position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005959"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00956"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF12718.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="333" mass="36372" checksum="45A8097141752133" modified="2006-05-30" version="1">MTTMSDARPRVFVAGHRGMVGSAIVRALAARGDVELVLRTHRELDLTEQRDVRHFFATERISQVYLAAARVGGIHANNVYPAEFIQQNLTIAANVIHEAWRGGVEQLLFLGSSCIYPKFASQPIQESALLTGALEPTNAPYAIAKIAGMMMCDSYNRQYGVDYRSVMPTNLYGPGDNYHPENSHVIPALIRRIHEARLADAPEVAVWGSGAPMREFLHADDLAHACLHVMDLPQASYRAHTQGAGFLNVGSGEEVSIRQLALLIARVVGYRGNIAFDPTRPDGTPRKMLDCGLINATGWQPQVSLESGLRSTYQEALSSGRLSVEPEALATPT</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="61" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LB00</accession>
    <name>Q1LB00_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Replicase protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">repA</name>
        <name evidence="1" type="ordered locus">Rmet_5817</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12676.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518029.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5817"/>
    <dbReference type="EnsemblBacteria" id="ABF12676">
        <property type="protein sequence ID" value="ABF12676"/>
        <property type="gene ID" value="Rmet_5817"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5817"/>
    <dbReference type="eggNOG" id="ENOG502ZB3T">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_051492_1_0_4"/>
    <dbReference type="OMA" id="RRTEIPW"/>
    <dbReference type="OrthoDB" id="1074108at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006881">
        <property type="entry name" value="RepA_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04796">
        <property type="entry name" value="RepA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF12676.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="364" mass="41123" checksum="199B1F667380EFCD" modified="2006-05-30" version="1">MPIKRSMAIDEDRSVLVGDNEPSRIIVPGNENGLVPSEKFQRLFDEAQAMEREDAWQSGEVGFLSRASVQVTLPYRAPKGAPPVWTRTSGNISLMIQPGYFTQQRSERATNGRQKLVSETVSLGYPYGSYPRLMLAWIGKEIMAKKKRGEFTGTVEDRRISLGNSLSEFMYNLGIPMATGGKRGTMTLVRQQMIRLFSATIAIVQNKSVPVPNQAPNHEPLSIDRVGYLLADQLSTWWDPMQPGQGSMFESFVVLSEPFFNELVNRPVPVDMRALKALKQSPFALDVYSWLTYRFFTIQRRTEIPWEALQMQFGTETESERKFRALFRKALKDVLVVYPGAKVDADSSKALILQPSRTSVRKLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-10-07" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LB77</accession>
    <name>Q1LB77_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_5740</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12599.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5740"/>
    <dbReference type="EnsemblBacteria" id="ABF12599">
        <property type="protein sequence ID" value="ABF12599"/>
        <property type="gene ID" value="Rmet_5740"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5740"/>
    <dbReference type="HOGENOM" id="CLU_2510304_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF12599.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="85" mass="9374" checksum="574D9CA0A9264561" modified="2006-05-30" version="1">MQKHTPQAQKKTPIFEMGVAGKDRWGQFNESASGSGNPLATSSIRIDNARINRTRRIDDMEESECIPLFADERGPNDLLTATALA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LB88</accession>
    <name>Q1LB88_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Polysaccharide export protein, polysialic acid transport protein kpsD</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">kpsD</name>
        <name evidence="5" type="ordered locus">Rmet_5729</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="6">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="6">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12588.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5729"/>
    <dbReference type="EnsemblBacteria" id="ABF12588">
        <property type="protein sequence ID" value="ABF12588"/>
        <property type="gene ID" value="Rmet_5729"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5729"/>
    <dbReference type="eggNOG" id="COG1596">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036993_0_0_4"/>
    <dbReference type="OMA" id="DRREFNY"/>
    <dbReference type="OrthoDB" id="687725at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015159">
        <property type="term" value="F:polysaccharide transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003715">
        <property type="entry name" value="Poly_export"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019554">
        <property type="entry name" value="Soluble_ligand-bd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02563">
        <property type="entry name" value="Poly_export"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10531">
        <property type="entry name" value="SLBB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0614">Plasmid</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193286" evidence="3">
        <location>
            <begin position="22"/>
            <end position="606"/>
        </location>
    </feature>
    <feature type="domain" description="SLBB" evidence="4">
        <location>
            <begin position="511"/>
            <end position="554"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="42"/>
            <end position="80"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF10531"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABF12588.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="606" mass="65154" checksum="8C72A83501FE5BF2" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="57" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LBA1</accession>
    <name>Q1LBA1_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_5716</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12575.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF12575">
        <property type="protein sequence ID" value="ABF12575"/>
        <property type="gene ID" value="Rmet_5716"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5716"/>
    <dbReference type="HOGENOM" id="CLU_1057156_0_0_4"/>
    <dbReference type="OMA" id="AVPTCCE"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF12575.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="263" mass="28262" checksum="CFCD9DCA080FC174" modified="2006-05-30" version="1">MATGIKQLHSTPGNPTTPSLGTGTDTQGTARMIRPSNVNYDLEELSAALHYNDEWLGRAKVAIEVLDQSNAHTAYARVIFFDKERAALCFHLAADIFRCAQLTRVVHRALGASFERVVPPSLLIDVGPIAHPDAALAGSPTVLADLTASVSMLANSTTEASWRQAAVCAYVVAFRLARKLIAAICPPTPNVLDSPLDTAIWQLRMHRSPKARALAEALASMQVTYSTAAFDFDTPFPQDQALLQSEAMKAFQADLAAFQATAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-10-07" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LBA2</accession>
    <name>Q1LBA2_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_5715</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12574.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5715"/>
    <dbReference type="EnsemblBacteria" id="ABF12574">
        <property type="protein sequence ID" value="ABF12574"/>
        <property type="gene ID" value="Rmet_5715"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5715"/>
    <dbReference type="HOGENOM" id="CLU_2234308_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="76"/>
            <end position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF12574.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="105" mass="11998" checksum="E7B18760463B924D" modified="2006-05-30" version="1">MSRVSCRLSGVMYVLYGTYSMGLRHWLWRAPILRRDEISLQRIRAGFQERSDRSASHTVSTENWCLLNHGVISLHVVMVFIASCFIAAISLVVFVACIFLLVSYD</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LBD3</accession>
    <name>Q1LBD3_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Pilus assembly protein, PilE</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">pilE</name>
        <name evidence="2" type="ordered locus">Rmet_5684</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12543.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011520085.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5684"/>
    <dbReference type="EnsemblBacteria" id="ABF12543">
        <property type="protein sequence ID" value="ABF12543"/>
        <property type="gene ID" value="Rmet_5684"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5684"/>
    <dbReference type="eggNOG" id="COG4968">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_091705_6_0_4"/>
    <dbReference type="OMA" id="AYPSYTN"/>
    <dbReference type="OrthoDB" id="1857712at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015627">
        <property type="term" value="C:type II protein secretion system complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015628">
        <property type="term" value="P:protein secretion by the type II secretion system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043683">
        <property type="term" value="P:type IV pilus assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000983">
        <property type="entry name" value="Bac_GSPG_pilin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012902">
        <property type="entry name" value="N_methyl_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031982">
        <property type="entry name" value="PilE-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045584">
        <property type="entry name" value="Pilin-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16732">
        <property type="entry name" value="ComP_DUS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07963">
        <property type="entry name" value="N_methyl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00813">
        <property type="entry name" value="BCTERIALGSPG"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54523">
        <property type="entry name" value="SSF54523"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02532">
        <property type="entry name" value="IV_pilin_GFxxxE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00409">
        <property type="entry name" value="PROKAR_NTER_METHYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="40"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF12543.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="167" mass="17656" checksum="FD3C93E23F96FC91" modified="2006-05-30" version="1">MGQPCPTVRHGHARTRGLFRDIGFTGDGARPRGSSRGFTLIEVMITVAIIGIIAAIAFPSYQRHVVKGNRAAAEAFVLEAASLQERYLVDNRAYATTLSALGFAALPDSIAPNYQVTLTVTAAPPAYVFTATPINTQLARDTDCGVLTLNNTGDKTAAGVASTSCWK</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LBU9</accession>
    <name>Q1LBU9_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Electron transfer flavoprotein, alpha subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">etfA</name>
        <name evidence="7" type="ordered locus">Rmet_5518</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="8">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="8">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ETF alpha-subunit/FixB family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12377.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519921.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5518"/>
    <dbReference type="EnsemblBacteria" id="ABF12377">
        <property type="protein sequence ID" value="ABF12377"/>
        <property type="gene ID" value="Rmet_5518"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5518"/>
    <dbReference type="eggNOG" id="COG2025">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_034178_0_0_4"/>
    <dbReference type="OMA" id="YPHQIGL"/>
    <dbReference type="OrthoDB" id="1400253at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01715">
        <property type="entry name" value="ETF_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029035">
        <property type="entry name" value="DHS-like_NAD/FAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014730">
        <property type="entry name" value="ETF_a/b_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001308">
        <property type="entry name" value="ETF_a/FixB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033947">
        <property type="entry name" value="ETF_alpha_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014731">
        <property type="entry name" value="ETF_asu_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018206">
        <property type="entry name" value="ETF_asu_C_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43153">
        <property type="entry name" value="PTHR43153"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01012">
        <property type="entry name" value="ETF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00766">
        <property type="entry name" value="ETF_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000089">
        <property type="entry name" value="Electra_flavoP_a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00893">
        <property type="entry name" value="ETF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52467">
        <property type="entry name" value="SSF52467"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00696">
        <property type="entry name" value="ETF_ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0249">Electron transport</keyword>
    <keyword evidence="4" id="KW-0274">FAD</keyword>
    <keyword evidence="3" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="7" id="KW-0614">Plasmid</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ETF" evidence="6">
        <location>
            <begin position="3"/>
            <end position="182"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005817"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABF12377.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="310" mass="31304" checksum="BEB7E2F6EE1CA331" modified="2006-05-30" version="1">MTALVIAEHDNQSIKAATLNAVTAAAQCGGDVHVLVAGSNAKAAADAAAKIAGVSKVLLADAPYFGDGLAENVAEQILAVASDYSHILAPATASGKNILPRVAAKLDVAQLSDITKVDSPDTFERPIYAGNAIAIVKSSDKIKVITVRGTGFDAAAAEGGSASVDTIPAVTDAGISQFVSREVTKSDRPELTAAKIIVSGGRGVGSGENYTKVLTPLADKLGAALGASRAAVDAGFVPNDYQVGQTGKIVAPQLYIAVGISGAIQHLAGMKDSKVIVAINKDAEAPIFSVADYGLVGDLNTMVPELVGSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LC59</accession>
    <name>Q1LC59_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Putative efflux outer membrane protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Rmet_5408</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="5">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="5">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the outer membrane factor (OMF) (TC 1.B.17) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12267.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519815.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5408"/>
    <dbReference type="EnsemblBacteria" id="ABF12267">
        <property type="protein sequence ID" value="ABF12267"/>
        <property type="gene ID" value="Rmet_5408"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5408"/>
    <dbReference type="eggNOG" id="COG1538">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023283_0_0_4"/>
    <dbReference type="OMA" id="TQMEQVR"/>
    <dbReference type="OrthoDB" id="1099555at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015562">
        <property type="term" value="F:efflux transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003423">
        <property type="entry name" value="OMP_efflux"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02321">
        <property type="entry name" value="OEP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0614">Plasmid</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193307" evidence="3">
        <location>
            <begin position="25"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="24"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="34"/>
            <end position="50"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF12267.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="506" mass="57214" checksum="C19AD8CF6657EDAD" modified="2006-05-30" version="1">MRKSSQPLAIALAATLLTTACSTAPSVPQPYSKEEVRQRVEEDRRGMYSDQEPVTRPITFYEAAARALKYNLDYRLKLMESTLSRQLVDVTKHDMLPKLVASAGYTHRSNDSGGVSVGIQDGQISLRPSTSEQRYHRLADLDLTWSTLDFLVAYERTQQKADQVLMAEERRRKVVQNVLQDVRNAYWRALGAQRLIGQVDALLNKVRQGLKAAQEADEKGLLPRQQALAYQRALLDAVSLLSARRQDLELARAELAALMSLPPDQPFTLADEAEQRMPNDRFDVAGLEQMALESRPEIMEEWYRKRVTDNDIKIAKAELWPSLSVDLGGHYDSNAYLYNNAWSAIGLRLSYNIFNLLKIPVLNEAQDSQNKVGDLRRMSLSMAVLTQVRVALQRYHLALEQLDFAEESLQVDNRLRRFAEASAQVSADSRLEFIRADARMLLAQYQRYAAYSEAQAAWGRLYNSVGLDVMPDAIASHDVPTLASEIEQTMQGWQQKLYPSAVDSKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LC62</accession>
    <name>Q1LC62_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_5405</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12264.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5405"/>
    <dbReference type="EnsemblBacteria" id="ABF12264">
        <property type="protein sequence ID" value="ABF12264"/>
        <property type="gene ID" value="Rmet_5405"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5405"/>
    <dbReference type="eggNOG" id="COG3900">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_074811_1_0_4"/>
    <dbReference type="OMA" id="CEHLAFR"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019207">
        <property type="entry name" value="DUF2092"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029046">
        <property type="entry name" value="LolA/LolB/LppX"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09865">
        <property type="entry name" value="DUF2092"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89392">
        <property type="entry name" value="SSF89392"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="25"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="72"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="26"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="72"/>
            <end position="86"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF12264.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="322" mass="35717" checksum="5E053C48B37B1231" modified="2006-05-30" version="1">MQPATSSDLVARTIHEYCSQPNPFFHGRQWRPSQQAMPTTGIRRRHPYPRGFAMNRALCIAALAISLGACSSPAPQATSSPAPDTQTAAKAPPPESKPDANAVNADAIQALKDMGGYLQTLKQFSVKIDLDGERVLEDGQKLQHSASARVDADRPDKMRVQMRSARAQRDLYYDGHKVALYYPESNYYSTVDSSDNLSNLVVKLRERFGIEVPVDDLFLWGTPNAPTNGITSAMVAGQDLIDGQLCDQYAFRQGMIDWQIWIASGNQPLPRKIVVTNRSDEARPQSVTWLTWNLKPKFTASTFHFTPPKGAKVAEFVPLKNQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LC90</accession>
    <name>Q1LC90_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Hemin ABC-transport system permease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">hmuU</name>
        <name evidence="8" type="ordered locus">Rmet_5377</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="9">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="9">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the binding-protein-dependent transport system permease family. FecCD subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12236.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519783.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5377"/>
    <dbReference type="EnsemblBacteria" id="ABF12236">
        <property type="protein sequence ID" value="ABF12236"/>
        <property type="gene ID" value="Rmet_5377"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5377"/>
    <dbReference type="eggNOG" id="COG0609">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013016_0_3_4"/>
    <dbReference type="OMA" id="MQGVFGN"/>
    <dbReference type="OrthoDB" id="1283908at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3470.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037294">
        <property type="entry name" value="ABC_BtuC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000522">
        <property type="entry name" value="ABC_transptr_permease_BtuC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30472">
        <property type="entry name" value="PTHR30472"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01032">
        <property type="entry name" value="FecCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81345">
        <property type="entry name" value="SSF81345"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0614">Plasmid</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="68"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="96"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="123"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="148"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="191"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="242"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="310"/>
            <end position="328"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007935"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ABF12236.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="333" mass="34821" checksum="19344EE976990C6D" modified="2006-05-30" version="1">MMRGQCGRRPGMLAALCVMLVITALWAAASGALSIPHGKLLPLLWSPAAEGEDVIWRNVLIEVRLPRIVLAITAGAALALSGAVMQALFRNPLAEPGLIGISLGGAAGAVAAIVLGAEGLGSVAPAAFAGSLIATAFAYVLGTRRPGAANLLLAGVAINAICAAVVGFFTYQASDVQLRNLTFWNMGSLAGATWSILAWLVPLVAVLCALLMRDWRAMNALLLGEREAQHIGFDLKRLRRRLIVLTALLVGPIVAVTGTISFVGLVVPHLIRLALGADHRWLLPNTLAGGAIALTLGDWMARTVVVPAELPIGIVTSLVGGPFLLWMLTRKRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="58" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LCQ1</accession>
    <name>Q1LCQ1_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">LssY_C domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_5216</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12075.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519620.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5216"/>
    <dbReference type="EnsemblBacteria" id="ABF12075">
        <property type="protein sequence ID" value="ABF12075"/>
        <property type="gene ID" value="Rmet_5216"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5216"/>
    <dbReference type="eggNOG" id="COG0671">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_703519_0_0_4"/>
    <dbReference type="OrthoDB" id="918168at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025902">
        <property type="entry name" value="LssY-like-C_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14067">
        <property type="entry name" value="LssY_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193007" description="LssY_C domain-containing protein" evidence="1">
        <location>
            <begin position="28"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="domain" description="LssY_C" evidence="2">
        <location>
            <begin position="266"/>
            <end position="376"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF14067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF12075.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="422" mass="46656" checksum="C681DF0A9F15517F" modified="2006-05-30" version="1">MSASGRLCVLLRRGASSALLLALGACATWQPPTDAGDGPLRERAISATSRDVRVSATVLGKEDSKRMLGADVTRMRVQPVWVEIHNGTAQPLWLLRSGTDPDYFSPLEVAWSLHTLLGGTINRRIDAYFNGLGFRNPIQPGQTRAGLLFTNPDYITKFVNIDFFGSNTLIPFTLYLQVPDDERSMAASRSLLIFRYPEADIQNYTDLASLRAALETLPACATDASGTGQGDPLNAVIVGEIDDISAAAVRRNYRRDLRQSDLPQRVLDRQPDVVTRKQAQAGAPAIWIRGWLTPIRFQGQPVFVAQVGRPVGGRFARTDDQSVLLQSDVDEARNLFVQDMMYSGGLEKLGFVYGVGAASLHYPRTTLGNATYHTDGLRAVLFFVTRPLSVSDVQFLDWVPYLEQRERFQSEESDDAARHAEH</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LCX8</accession>
    <name>Q1LCX8_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Anaerobic ribonucleoside triphosphate reductase</fullName>
            <ecNumber evidence="1">1.17.4.2</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">nrdD</name>
        <name evidence="1" type="ordered locus">Rmet_5139</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EC" id="1.17.4.2" evidence="1"/>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11998.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519545.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5139"/>
    <dbReference type="EnsemblBacteria" id="ABF11998">
        <property type="protein sequence ID" value="ABF11998"/>
        <property type="gene ID" value="Rmet_5139"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5139"/>
    <dbReference type="eggNOG" id="COG1328">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002707_0_2_4"/>
    <dbReference type="OMA" id="YSYDRVP"/>
    <dbReference type="OrthoDB" id="195920at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008998">
        <property type="term" value="F:ribonucleoside-triphosphate reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01675">
        <property type="entry name" value="RNR_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012833">
        <property type="entry name" value="NrdD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13597">
        <property type="entry name" value="NRDD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02487">
        <property type="entry name" value="NrdD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF11998.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="574" mass="64703" checksum="023CC7748E5319D0" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LD04</accession>
    <name>Q1LD04_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Extra-cytoplasmic solute receptor protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">bug</name>
        <name evidence="4" type="ordered locus">Rmet_5113</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="5">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="5">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Periplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the UPF0065 (bug) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11972.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519519.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5113"/>
    <dbReference type="EnsemblBacteria" id="ABF11972">
        <property type="protein sequence ID" value="ABF11972"/>
        <property type="gene ID" value="Rmet_5113"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5113"/>
    <dbReference type="eggNOG" id="COG3181">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045683_0_0_4"/>
    <dbReference type="OMA" id="HRARSMA"/>
    <dbReference type="OrthoDB" id="447734at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042597">
        <property type="term" value="C:periplasmic space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005064">
        <property type="entry name" value="BUG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042100">
        <property type="entry name" value="Bug_dom1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42928">
        <property type="entry name" value="PTHR42928"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03401">
        <property type="entry name" value="TctC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017082">
        <property type="entry name" value="YflP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-0675">Receptor</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193326" evidence="3">
        <location>
            <begin position="31"/>
            <end position="330"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF11972.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="330" mass="35229" checksum="DD62A73F0470D545" modified="2006-05-30" version="1">MQHGFRRRRLMAAIGFSFALGGTSLAPAHADTWPTKPVTIVVAFAPGGMTDIVGRMLAAELSQTFKQSFVVENRPGAAGQLATEYVARKPNDGYTLLLSATGHVIGPAVQKTVRYRPVLDFEPIALIAKAPNMLVVNPSVPAHTVPEFVTWAKAQGSVPYGSAGVGGSTHLAGELFRHMTGAPLVHVPYKGASPSTSDAVAGQIPVVFQDSMSVSAFIAAGKLRPIAVTSLERSKLFPDVPTIAEAGYKNFDAYTWLGLYAPTGTPAAIVTRLNTEVNRIMSSPDMVAKLKKQNAEAAGNLNPAEFRKYVETEVAKWQNMVKITGVQIEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="63" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LD96</accession>
    <name>Q1LD96_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_5018</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11880.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519429.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5018"/>
    <dbReference type="EnsemblBacteria" id="ABF11880">
        <property type="protein sequence ID" value="ABF11880"/>
        <property type="gene ID" value="Rmet_5018"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5018"/>
    <dbReference type="HOGENOM" id="CLU_1183441_0_0_4"/>
    <dbReference type="OMA" id="PLCRRCE"/>
    <dbReference type="OrthoDB" id="1969687at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF11880.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="212" mass="23351" checksum="F20B65D1B576E4AC" modified="2006-05-30" version="1">MPGAQRIAQTTPDAGDLRREVRALTRRAHRMALAQACATEAYEDRLAALFDAWMATGDGRQRVVCFATAAQFGLDVRRYAESCRQAATCGGGEAVRMLYLAAARRLRSRWGEAARRLDIGDATVAAAARVRAMPATAEGPLCTHCERLRHDSRRAGGHGALRPAGDLQWRSLGGSAMCETRAHQCMLCRTDWMRHRTGADPFVGWTISRRPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LDJ9</accession>
    <name>Q1LDJ9_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative periplasmic copper-binding</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">nosD</name>
        <name evidence="2" type="ordered locus">Rmet_4915</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11777.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519336.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4915"/>
    <dbReference type="EnsemblBacteria" id="ABF11777">
        <property type="protein sequence ID" value="ABF11777"/>
        <property type="gene ID" value="Rmet_4915"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4915"/>
    <dbReference type="eggNOG" id="COG3420">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041882_0_1_4"/>
    <dbReference type="OMA" id="RYGLHFM"/>
    <dbReference type="OrthoDB" id="1177619at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.20.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006633">
        <property type="entry name" value="Carb-bd_sugar_hydrolysis-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026464">
        <property type="entry name" value="NosD_copper_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007742">
        <property type="entry name" value="NosD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022441">
        <property type="entry name" value="Para_beta_helix_rpt-2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006626">
        <property type="entry name" value="PbH1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012334">
        <property type="entry name" value="Pectin_lyas_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011050">
        <property type="entry name" value="Pectin_lyase_fold/virulence"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05048">
        <property type="entry name" value="NosD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00722">
        <property type="entry name" value="CASH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00710">
        <property type="entry name" value="PbH1"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51126">
        <property type="entry name" value="SSF51126"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR04247">
        <property type="entry name" value="NosD_copper_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03804">
        <property type="entry name" value="para_beta_helix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CASH" evidence="1">
        <location>
            <begin position="37"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="domain" description="CASH" evidence="1">
        <location>
            <begin position="191"/>
            <end position="356"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF11777.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="425" mass="47593" checksum="47626328F2E38289" modified="2006-05-30" version="1">MKWVPILILLAGWLTGAQGATVRVAPGRPLQAAIDAARPGDVLEIERATYTGNFVIGKALTLRGVDRPTISGGQHGDTLRVTASDVVIEGLIVRDSGDSLKDQNAGINILPGAHRAVVRHCDLTYNLFGLWIEKADDVRVEGNTITGKRDYNSAQRGNGVQLYNTKGARIIDNNISFVRDALYVDVSHHAIFKGNRLHHSRYGTHYMNSYYNLWEDNDSYNNRGGLALMEVRDQVVRNNRTWGNADHGIMLRTLQDSRVENNVVADNDRGFFIYDVEYTELRGNLVIGNQVGVHLSAGSTRNVVQGNDFIGNREQVRYLGARDEPWGGRKPSERDQGNFWSDYLGWDRDGDGIGDLPYEASDVLDRLIWRHPGVQLLLASPAVQALRFVSRQFPVLRVPTVVDAYPRAQPVNKNWSTWRDRAHSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LDZ6</accession>
    <name>Q1LDZ6_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">2-dehydro-3-deoxy-phosphogluconate aldolase</fullName>
            <ecNumber evidence="3">4.1.2.14</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">eda</name>
        <name evidence="5" type="ordered locus">Rmet_4768</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="6">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="6">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>2-dehydro-3-deoxy-6-phospho-D-gluconate = D-glyceraldehyde 3-phosphate + pyruvate</text>
            <dbReference type="Rhea" id="RHEA:17089"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:57569"/>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
            <dbReference type="EC" id="4.1.2.14"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the KHG/KDPG aldolase family.</text>
    </comment>
    <dbReference type="EC" id="4.1.2.14" evidence="3"/>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11630.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519200.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4768"/>
    <dbReference type="EnsemblBacteria" id="ABF11630">
        <property type="protein sequence ID" value="ABF11630"/>
        <property type="gene ID" value="Rmet_4768"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4768"/>
    <dbReference type="eggNOG" id="COG0800">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_077795_1_1_4"/>
    <dbReference type="OMA" id="FFPAEYC"/>
    <dbReference type="OrthoDB" id="1497385at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008675">
        <property type="term" value="F:2-dehydro-3-deoxy-phosphogluconate aldolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd00452">
        <property type="entry name" value="KDPG_aldolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000887">
        <property type="entry name" value="Aldlse_KDPG_KHG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031337">
        <property type="entry name" value="KDPG/KHG_AS_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031338">
        <property type="entry name" value="KDPG/KHG_AS_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30246">
        <property type="entry name" value="PTHR30246"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01081">
        <property type="entry name" value="Aldolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01182">
        <property type="entry name" value="eda"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00159">
        <property type="entry name" value="ALDOLASE_KDPG_KHG_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00160">
        <property type="entry name" value="ALDOLASE_KDPG_KHG_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0456">Lyase</keyword>
    <keyword evidence="5" id="KW-0614">Plasmid</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000654"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABF11630.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="213" mass="22139" checksum="3834D8355B2B1479" modified="2006-05-30" version="1">MPIHASPLLERLTNVPVIPVLEYHSVDDALHISEALVAGGLPLLEITLRTPVALQAMEAVAKALPEAVVGAGTVLTTDQLQAVRDAGAQFAVSPGLTQKLADGAQDAGISLLPGVATASEAMFALEAGFSFLKFFPAEAAGGVQMLKSLHGPFSQLKFCPTGGIDLAKAPNYLALPNVVCVGGSWVVPKDAVAAKDWGRIRKLAEEAAQLRKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="95" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LEF1</accession>
    <name>Q1LEF1_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Acyl-CoA dehydrogenase-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Rmet_4613</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="9">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="9">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the acyl-CoA dehydrogenase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11475.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519050.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4613"/>
    <dbReference type="EnsemblBacteria" id="ABF11475">
        <property type="protein sequence ID" value="ABF11475"/>
        <property type="gene ID" value="Rmet_4613"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4613"/>
    <dbReference type="eggNOG" id="COG1960">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018204_1_0_4"/>
    <dbReference type="OMA" id="WIATGIQ"/>
    <dbReference type="OrthoDB" id="760677at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016627">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-CH group of donors"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.540.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.110.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006091">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046373">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036250">
        <property type="entry name" value="AcylCo_DH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009075">
        <property type="entry name" value="AcylCo_DH/oxidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013786">
        <property type="entry name" value="AcylCoA_DH/ox_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037069">
        <property type="entry name" value="AcylCoA_DH/ox_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009100">
        <property type="entry name" value="AcylCoA_DH/oxidase_NM_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00441">
        <property type="entry name" value="Acyl-CoA_dh_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02770">
        <property type="entry name" value="Acyl-CoA_dh_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02771">
        <property type="entry name" value="Acyl-CoA_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47203">
        <property type="entry name" value="SSF47203"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56645">
        <property type="entry name" value="SSF56645"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0274">FAD</keyword>
    <keyword evidence="3" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="8" id="KW-0614">Plasmid</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Acyl-CoA_dh_N" evidence="7">
        <location>
            <begin position="7"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA_dh_M" evidence="6">
        <location>
            <begin position="128"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA_dh_1" evidence="5">
        <location>
            <begin position="238"/>
            <end position="385"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF02770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF02771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ABF11475.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="397" mass="43074" checksum="E87859F913A7DEF7" modified="2006-05-30" version="1">MFTPCPTERSRDIADRVERFVRDVVAPYEQDPRCSAHGPTEELVLELRAKARAAGIMTPHILPDGGHLSQLETAAVLKRSGLSPLGPVAVNTMAPDEGNMFLLGKVATAAQKARFLEPLVRGEARSAFFMTEPAEEGGAGSDPSMLSTTAVRDGNDWVLNGRKKFITGAEGARVGIVMARTGDGERAQATMFLVDLPHPAIRTERVIDTIDSSMPGGHAQVLIDGLRLPADQVLGEVNEGFRYAQVRLSPARLSHCMRWFGTVTRADEIARAYATTRKAFGKLLIDHEGVGFMLADNLIDLQQAALMIDWCAGVLDTGSAGTTESSMAKVAVSEALFRVADRCVQILGGNGVSRDTIVDQAFRELRAFRIYDGPTEVHKWSLAKKIKRDTLQSSHHG</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="96" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LEI8</accession>
    <name>Q1LEI8_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Peptide transporter permease subunit: membrane component of ABC superfamily</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">dppB</name>
        <name evidence="7" type="ordered locus">Rmet_4576</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="8">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="8">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cell membrane</location>
            <topology evidence="5">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the binding-protein-dependent transport system permease family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11438.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008648520.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4576"/>
    <dbReference type="EnsemblBacteria" id="ABF11438">
        <property type="protein sequence ID" value="ABF11438"/>
        <property type="gene ID" value="Rmet_4576"/>
    </dbReference>
    <dbReference type="GeneID" id="60825954"/>
    <dbReference type="KEGG" id="rme:Rmet_4576"/>
    <dbReference type="eggNOG" id="COG0601">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036879_0_0_4"/>
    <dbReference type="OMA" id="IFTAQYT"/>
    <dbReference type="OrthoDB" id="1479518at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045621">
        <property type="entry name" value="BPD_transp_1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19300">
        <property type="entry name" value="BPD_transp_1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="SSF161098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0614">Plasmid</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="9"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="99"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="141"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="185"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="239"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="285"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="6">
        <location>
            <begin position="99"/>
            <end position="304"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABF11438.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="318" mass="35409" checksum="DA2EF5A638B80F96" modified="2006-05-30" version="1">MSAYFVRRLLALIPTLLFASIIVFTIVRLVPGDVVDLMLSQNDISADALTREQLVHALGLDRPIWDQYLRWVTNIVLHGDLGQSLWQSEPVLKMVMARIPATFSLGVLALFVALTVALPIGIVSATRQDSAVDYVARSFSILMLAVPSFWLGTMVIVFPSVWWGWSPDVRYIPFLEDPVQHVKQMLVPAIVLGMALSAITMRMTRTMMLEVLRQDYIRTAWAKGLNERQVIFRHALRNALIPVVTLIGLQAPLLIGGAVVIEQIFAVPGMGLLLLDAVHQRDYPVITGVFLVVGVAVMLINLLVDLSYGLFDPKVRHH</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LEP4</accession>
    <name>Q1LEP4_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Universal stress protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">uspA12</name>
        <name evidence="3" type="ordered locus">Rmet_4517</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal stress protein A family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11382.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518975.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4517"/>
    <dbReference type="EnsemblBacteria" id="ABF11382">
        <property type="protein sequence ID" value="ABF11382"/>
        <property type="gene ID" value="Rmet_4517"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4517"/>
    <dbReference type="eggNOG" id="COG0589">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_049301_11_0_4"/>
    <dbReference type="OMA" id="LAIDGSH"/>
    <dbReference type="OrthoDB" id="2040681at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006015">
        <property type="entry name" value="Universal_stress_UspA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006016">
        <property type="entry name" value="UspA"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00582">
        <property type="entry name" value="Usp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01438">
        <property type="entry name" value="UNVRSLSTRESS"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Usp" evidence="2">
        <location>
            <begin position="1"/>
            <end position="146"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008791"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF11382.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="150" mass="15685" checksum="9C8542241C7B929F" modified="2006-05-30" version="1">MYERILVAVDGSPSSDLAIAQAAGLAKGMGAEVKVLFVVDEGEVFLEAGFVNPQDILNGLTDFGKKALAAAARRLQDAGVRFQTELAEKSAVPGDIAGAIVGYADRWPADVIVMGTRGRRGVRRMIMGSVSEGVVAKTSKPVLLIRGEAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="96" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LF14</accession>
    <name>Q1LF14_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Binding-protein-dependent transport systems inner membrane component</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">potI</name>
        <name evidence="6" type="ordered locus">Rmet_4397</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="7">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="7">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cell membrane</location>
            <topology evidence="4">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the binding-protein-dependent transport system permease family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11262.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518879.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4397"/>
    <dbReference type="EnsemblBacteria" id="ABF11262">
        <property type="protein sequence ID" value="ABF11262"/>
        <property type="gene ID" value="Rmet_4397"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4397"/>
    <dbReference type="eggNOG" id="COG1177">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016047_3_0_4"/>
    <dbReference type="OMA" id="NKFGMKW"/>
    <dbReference type="OrthoDB" id="1264541at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="SSF161098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0614">Plasmid</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2 4" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="7"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="67"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="95"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="128"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="172"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="230"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="5">
        <location>
            <begin position="63"/>
            <end position="248"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABF11262.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="260" mass="28076" checksum="7927E686DB5702F7" modified="2006-05-30" version="1">MNRPRWPVWVACLGCAVYAFLYLPLLIVVAYSFNDSRLNAEWDGFTLHWYDVLASDPDMLGAAWNSLMIGAIAAGTSCVLGTLAAIALHRYRPPLLATLVLAPIAMPEVLMGVSLLLFFVAVNIDLGLTAVALSHITFCVGFVAVVVRARLEGLDESLIDAARDLGASPLQAGWHVMLPLMLPGVLAGALLAFTLSMDDFVVTFFTAGPGQPTLPLQIYSMIRIAVTPEVNAISSILMMLTLAVSLTAYRVSRDVWRAQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LF24</accession>
    <name>Q1LF24_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Monooxygenase, FAD-binding:FAD dependent oxidoreductase:Tryptophan halogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_4387</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11252.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518869.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4387"/>
    <dbReference type="EnsemblBacteria" id="ABF11252">
        <property type="protein sequence ID" value="ABF11252"/>
        <property type="gene ID" value="Rmet_4387"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4387"/>
    <dbReference type="eggNOG" id="COG0644">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024648_4_1_4"/>
    <dbReference type="OMA" id="LPMAFNR"/>
    <dbReference type="OrthoDB" id="1770293at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002938">
        <property type="entry name" value="FAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01494">
        <property type="entry name" value="FAD_binding_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="SSF51905"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD_binding_3" evidence="1">
        <location>
            <begin position="6"/>
            <end position="165"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF11252.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="409" mass="45307" checksum="813EBE326632D13D" modified="2006-05-30" version="1">MKKETVDVLIVGAGPAGSVAAGLLRKRGIGVLVIEKETFPRFSIGESLLPQSMAYIEEAGMLQAVVEAGFQYKNGAAFARGDRYTDFDFREKFSPGWGTTYQVQRAHFDDVLIREAQKQGAEVRFRHVVEAVDVSGAAPVVTVRDPDGNVYQVESRFLLDASGFGRVLPRLLDLESPSNFPVRAAIFTHVADRIPGGSFDRNKIRVSVHPEHVDVWYWTIPFSNGRCSQGVVAEKSFLDRYEGDEMTRLRQLVAEEPGLAKLLKDAEWDTPARQIVGYSANVRSLWGNGYALLGNAGEFLDPVFSSGVTIAFKSASLATACVARALAGESVDWETEYARPLKAGVDCFRAFVEAWYEGSFQKLIFHPNAPTDIRDMISSILAGYAWDLNNPFVTESRRRLSVLEQFCDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LFI8</accession>
    <name>Q1LFI8_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Outer membrane protein (Porin)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">omp</name>
        <name evidence="5" type="ordered locus">Rmet_4221</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="6">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="6">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11088.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518711.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4221"/>
    <dbReference type="EnsemblBacteria" id="ABF11088">
        <property type="protein sequence ID" value="ABF11088"/>
        <property type="gene ID" value="Rmet_4221"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4221"/>
    <dbReference type="eggNOG" id="COG3203">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038238_2_0_4"/>
    <dbReference type="OMA" id="GYINTEA"/>
    <dbReference type="OrthoDB" id="1064531at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046930">
        <property type="term" value="C:pore complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015288">
        <property type="term" value="F:porin activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006811">
        <property type="term" value="P:ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00342">
        <property type="entry name" value="gram_neg_porins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.160.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033900">
        <property type="entry name" value="Gram_neg_porin_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023614">
        <property type="entry name" value="Porin_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13609">
        <property type="entry name" value="Porin_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0406">Ion transport</keyword>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-0626">Porin</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1134">Transmembrane beta strand</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Porin_4" evidence="4">
        <location>
            <begin position="9"/>
            <end position="328"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF13609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABF11088.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="362" mass="38262" checksum="476BCF6AA8A4454E" modified="2006-05-30" version="1">MKARLTVPALAALPCCVSAESRANVTLYGVVDAGVSFTNNQVGGGSVVRQTSGSLSSSRWGVRGSEDLGQGLKAVFVLESGFDVDTGKSAQGGRLFGRSAYVGLESQWGSILFGRQQSALFDMIGAFDPMALSPRWSIFNQDLALVARPDNTMKYVGKFGGLTATAMYSFGAESTTVDGSEVPGNAKLGREFGFRLSYNSGPFSIGTAYDEVNTGTLTTNRDATSRRATVAGTYALSTLKVFAGYRWAKAYDGAILVGALPGVSNQASNLWWTGVMWQAGPAVTLSAAAYYQDFRNTGGDPWLFVATGRYAFSKRTEAYLTLGYALNQENSNMGLGNGAAGFGHTPPGSNQFGTTLGIKHIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LFM1</accession>
    <name>Q1LFM1_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Transcriptional regulator, TetR family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_4188</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11055.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF11055">
        <property type="protein sequence ID" value="ABF11055"/>
        <property type="gene ID" value="Rmet_4188"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4188"/>
    <dbReference type="eggNOG" id="COG1309">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069356_28_4_4"/>
    <dbReference type="OMA" id="FMATVHG"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001647">
        <property type="entry name" value="HTH_TetR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036271">
        <property type="entry name" value="Tet_transcr_reg_TetR-rel_C_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00440">
        <property type="entry name" value="TetR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48498">
        <property type="entry name" value="SSF48498"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50977">
        <property type="entry name" value="HTH_TETR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH tetR-type" evidence="2">
        <location>
            <begin position="16"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="H-T-H motif" evidence="1">
        <location>
            <begin position="39"/>
            <end position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF11055.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="211" mass="23615" checksum="D577996F1AFDB12B" modified="2006-05-30" version="1">MCIYLRVDKVATMKTQPVREQLLEHTLILIRQRGCNGFSYRDLAELVGVKTSSIHYYFPSKDDLILEAVKEYSRRISERLAGISADLPVLDQARQYLEPLRQTCGSNQICLAGMLSTEILSLPESVHSVLKDFYHVNENWLAKLLERGQKERDTPYPVPPAMLAQVIYGALQSGLIAARLFGTQDRLESAAETLLGALCNKRAAALLEAHA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="107" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LFV5</accession>
    <name>Q1LFV5_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Sigma54 specific transcriptional regulator</fullName>
            <ecNumber evidence="7">6.4.1.6</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">acxR</name>
        <name evidence="7" type="ordered locus">Rmet_4104</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="8">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="8">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EC" id="6.4.1.6" evidence="7"/>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10971.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518599.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4104"/>
    <dbReference type="EnsemblBacteria" id="ABF10971">
        <property type="protein sequence ID" value="ABF10971"/>
        <property type="gene ID" value="Rmet_4104"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4104"/>
    <dbReference type="eggNOG" id="COG3284">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_8_12_4"/>
    <dbReference type="OMA" id="NCGGMAR"/>
    <dbReference type="OrthoDB" id="123059at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018710">
        <property type="term" value="F:acetone carboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003018">
        <property type="entry name" value="GAF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029016">
        <property type="entry name" value="GAF-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002197">
        <property type="entry name" value="HTH_Fis"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002078">
        <property type="entry name" value="Sigma_54_int"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025662">
        <property type="entry name" value="Sigma_54_int_dom_ATP-bd_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025943">
        <property type="entry name" value="Sigma_54_int_dom_ATP-bd_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025944">
        <property type="entry name" value="Sigma_54_int_dom_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01590">
        <property type="entry name" value="GAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02954">
        <property type="entry name" value="HTH_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00158">
        <property type="entry name" value="Sigma54_activat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01590">
        <property type="entry name" value="HTHFIS"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00675">
        <property type="entry name" value="SIGMA54_INTERACT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00676">
        <property type="entry name" value="SIGMA54_INTERACT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00688">
        <property type="entry name" value="SIGMA54_INTERACT_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50045">
        <property type="entry name" value="SIGMA54_INTERACT_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-0436">Ligase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0614">Plasmid</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Sigma-54 factor interaction" evidence="6">
        <location>
            <begin position="350"/>
            <end position="580"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50045"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABF10971.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="661" mass="72024" checksum="1259079F2AE3CE54" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="96" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LGA1</accession>
    <name>Q1LGA1_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Cytochrome c oxidase subunit III</fullName>
            <ecNumber evidence="10">1.9.3.1</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">Rmet_3957</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="11">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="11">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cell membrane</location>
            <topology evidence="6">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the cytochrome c oxidase subunit 3 family.</text>
    </comment>
    <dbReference type="EC" id="1.9.3.1" evidence="10"/>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10825.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518464.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3957"/>
    <dbReference type="EnsemblBacteria" id="ABF10825">
        <property type="protein sequence ID" value="ABF10825"/>
        <property type="gene ID" value="Rmet_3957"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3957"/>
    <dbReference type="eggNOG" id="COG1845">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_044071_4_0_4"/>
    <dbReference type="OMA" id="CARYWHF"/>
    <dbReference type="OrthoDB" id="1502742at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004129">
        <property type="term" value="F:cytochrome-c oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019646">
        <property type="term" value="P:aerobic electron transport chain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024791">
        <property type="entry name" value="Cyt_c/ubiquinol_Oxase_su3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000298">
        <property type="entry name" value="Cyt_c_oxidase-like_su3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035973">
        <property type="entry name" value="Cyt_c_oxidase_su3-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013833">
        <property type="entry name" value="Cyt_c_oxidase_su3_a-hlx"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11403">
        <property type="entry name" value="PTHR11403"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81452">
        <property type="entry name" value="SSF81452"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50253">
        <property type="entry name" value="COX3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-0614">Plasmid</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="43"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="77"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="116"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="150"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="195"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="domain" description="COX3" evidence="9">
        <location>
            <begin position="1"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010581"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003376"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50253"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ABF10825.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="222" mass="23770" checksum="E392B67BB89C5C19" modified="2006-05-30" version="1">MKAESFAVDTGGGVFRASREAPGGSPAGGDALHGHGARTPGSLGLWVFMGVVTALFTLFLAAYIMRMDSPDWQRIALPWQVWLSTAVLAAACVAMEIARRAAHSGRMVRARHAFRLGGMGAVAFVGVQLWAWQALYALHVVPANNPAGSFFYLLTAMHGLHMLGGLVAFAFVAVDRGSGTRTVTRIGLCARYWHFLLAVWVVLLGALGWLTPELVAYICGRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LGR4</accession>
    <name>Q1LGR4_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Extra-cytoplasmic solute receptor protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">bug</name>
        <name evidence="4" type="ordered locus">Rmet_3792</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="5">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="5">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Periplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the UPF0065 (bug) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10662.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518310.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3792"/>
    <dbReference type="EnsemblBacteria" id="ABF10662">
        <property type="protein sequence ID" value="ABF10662"/>
        <property type="gene ID" value="Rmet_3792"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3792"/>
    <dbReference type="eggNOG" id="COG3181">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045683_0_2_4"/>
    <dbReference type="OMA" id="TLTINPW"/>
    <dbReference type="OrthoDB" id="447734at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042597">
        <property type="term" value="C:periplasmic space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005064">
        <property type="entry name" value="BUG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042100">
        <property type="entry name" value="Bug_dom1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42928">
        <property type="entry name" value="PTHR42928"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03401">
        <property type="entry name" value="TctC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017082">
        <property type="entry name" value="YflP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-0675">Receptor</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193112" evidence="3">
        <location>
            <begin position="27"/>
            <end position="326"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF10662.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="326" mass="33757" checksum="6D8D9B55CF37F7BE" modified="2006-05-30" version="1">MNLPRRAALAHSIAASLLFCVANAGAQEFPSQPIRIVVPYAAGGMTDIVARVIGQKLGEHLGQPVVIDNRPGAATIVGAEVVAGAKPDGYTLLAATNTTLTINPWLYPKLRYDAGKSFTPIALVASAPSVIVVNPLVPAKTLSELMQLAKARHGGLSYASYGNGSTAHLAGEMLAARAGAELLHVPYKGSAPAKAAVIAGEVNMTFEPAFTGLPQISSGKLRALAVMSSKRSAVLPDVPTTAELGYPGMEMAAWVGLMAPAGTPTPIVKKLASEVEHALTDPAVLETLLKNGAEPAGYYGGSFAAYLRDETARYGKVIADARIHAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="69" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LGT3</accession>
    <name>Q1LGT3_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Tn7-like transposition protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">tnsD</name>
        <name evidence="2" type="ordered locus">Rmet_3773</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10643.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518288.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3773"/>
    <dbReference type="EnsemblBacteria" id="ABF10643">
        <property type="protein sequence ID" value="ABF10643"/>
        <property type="gene ID" value="Rmet_3773"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3773"/>
    <dbReference type="eggNOG" id="COG3677">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033785_0_0_4"/>
    <dbReference type="OMA" id="WPRETIA"/>
    <dbReference type="OrthoDB" id="1446068at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009492">
        <property type="entry name" value="TniQ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032750">
        <property type="entry name" value="TnsD_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06527">
        <property type="entry name" value="TniQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15978">
        <property type="entry name" value="TnsD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TnsD" evidence="1">
        <location>
            <begin position="229"/>
            <end position="432"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF15978"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF10643.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="435" mass="50108" checksum="9A9C7E38AA9AB170" modified="2006-05-30" version="1">MNVFMSPAYDDELVYNVCTRYLAEFPTVSRERIITDVFGVNIVLSPHLPRNLHQFCERTGQVIAKTKEEMFWWHSMYPFICAYQTSERRAQARDYAFYGRAVRRMPGILAAIEPPKFLRWCPDCVVEDRARPDCGEAYWRRTHQIPGVWQCDKHGTDLVDSQVPRFASVTSDHPAEMMIPRNVRPFRKKRPCSPMEALLRKHLVALLKAGHFEDRPEHPNFREAAARAGFGPTVKIDYTELYEAFSEYWGDVLERLDERYRTRPAGVSWLHAQFNARGLMGQPVVRELFDIFFRDRCGLDVYQMPPLESSYKRFMPVFYCPNPYAAHGRGEQVKRVTRCKDNPDILDISCKCGFTAMVSAASAGKTMTMADVIKVGKFGPTWPEQCAKLRAEGKGLSQVAKELNLPENTARYLWRQSKPLLAADEKTLKKQESAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-12-02" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LGU0</accession>
    <name>Q1LGU0_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">DNA-3-methyladenine glycosylase</fullName>
            <ecNumber evidence="2">3.2.2.21</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">alkA</name>
        <name evidence="2" type="ordered locus">Rmet_3766</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EC" id="3.2.2.21" evidence="2"/>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10636.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3766"/>
    <dbReference type="EnsemblBacteria" id="ABF10636">
        <property type="protein sequence ID" value="ABF10636"/>
        <property type="gene ID" value="Rmet_3766"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3766"/>
    <dbReference type="eggNOG" id="COG0122">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_72_5_4"/>
    <dbReference type="OMA" id="RTVAAWY"/>
    <dbReference type="OrthoDB" id="1781735at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008725">
        <property type="term" value="F:DNA-3-methyladenine glycosylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052822">
        <property type="term" value="F:DNA-3-methylguanine glycosylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052821">
        <property type="term" value="F:DNA-7-methyladenine glycosylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043916">
        <property type="term" value="F:DNA-7-methylguanine glycosylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006284">
        <property type="term" value="P:base-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00056">
        <property type="entry name" value="ENDO3c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011257">
        <property type="entry name" value="DNA_glycosylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003265">
        <property type="entry name" value="HhH-GPD_domain"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00730">
        <property type="entry name" value="HhH-GPD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00478">
        <property type="entry name" value="ENDO3c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48150">
        <property type="entry name" value="SSF48150"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ENDO3c" evidence="1">
        <location>
            <begin position="61"/>
            <end position="217"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF10636.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="218" mass="24413" checksum="C7B0CC71A3A54472" modified="2006-05-30" version="1">MSGIHAKTIAADLSPLAYRRAVKFLSELDEDWALHIAAIGPCRHEAKPAREPYEALVRAIAYQQLHARAGDAILGRLLALYPGVSFPAPEQLLASDPELQRACGFSAEKLATIRRIAQATVEGLVPNLEEARRLPDTALIERLTSLRGVGRWTVEMFLIYSLERSDVLPVDDFGVREGYGRLKGLERAPTPRQMREIGEAWSPFRTVAAWYLWRLPSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="53" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LGU4</accession>
    <name>Q1LGU4_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_3762</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10632.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF10632">
        <property type="protein sequence ID" value="ABF10632"/>
        <property type="gene ID" value="Rmet_3762"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3762"/>
    <dbReference type="HOGENOM" id="CLU_1968746_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="37"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="38"/>
            <end position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF10632.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="127" mass="13547" checksum="A6407A532C6D51FB" modified="2006-05-30" version="1">MYPERAALLQSEVHVMKNNRMLAVACGTFIVALSAAHAQPTQAPNSGANPSYSNSQGKFDPYTQGANTDSTKSNLSPSATPSSPMDPSTPMQGTSQVNPYPQGVRQDPQFNPGGHVYDVRNPFFMGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="92" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LGW6</accession>
    <name>Q1LGW6_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Flagellar basal-body rod protein FlgF</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">flgF</name>
        <name evidence="7" type="ordered locus">Rmet_3738</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="8">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="8">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="4">The basal body constitutes a major portion of the flagellar organelle and consists of five rings (E,L,P,S, and M) mounted on a central rod. The rod consists of about 26 subunits of FlgG in the distal portion, and FlgB, FlgC and FlgF are thought to build up the proximal portion of the rod with about 6 subunits each.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 4">Bacterial flagellum basal body</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 4">Belongs to the flagella basal body rod proteins family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10610.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518257.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3738"/>
    <dbReference type="EnsemblBacteria" id="ABF10610">
        <property type="protein sequence ID" value="ABF10610"/>
        <property type="gene ID" value="Rmet_3738"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3738"/>
    <dbReference type="eggNOG" id="COG4787">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013687_1_0_4"/>
    <dbReference type="OMA" id="NLYFAIQ"/>
    <dbReference type="OrthoDB" id="947161at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030694">
        <property type="term" value="C:bacterial-type flagellum basal body, rod"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071973">
        <property type="term" value="P:bacterial-type flagellum-dependent cell motility"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001444">
        <property type="entry name" value="Flag_bb_rod_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019776">
        <property type="entry name" value="Flagellar_basal_body_rod_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020013">
        <property type="entry name" value="Flagellar_FlgE/F/G"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010930">
        <property type="entry name" value="Flg_bb/hook_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037925">
        <property type="entry name" value="FlgE/F/G-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012836">
        <property type="entry name" value="FlgF"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00460">
        <property type="entry name" value="Flg_bb_rod"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06429">
        <property type="entry name" value="Flg_bbr_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117143">
        <property type="entry name" value="SSF117143"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03506">
        <property type="entry name" value="FlgEFG_subfam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02490">
        <property type="entry name" value="flgF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00588">
        <property type="entry name" value="FLAGELLA_BB_ROD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0975">Bacterial flagellum</keyword>
    <keyword evidence="7" id="KW-0966">Cell projection</keyword>
    <keyword evidence="7" id="KW-0969">Cilium</keyword>
    <keyword evidence="7" id="KW-0282">Flagellum</keyword>
    <keyword evidence="7" id="KW-0614">Plasmid</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Flg_bb_rod" evidence="5">
        <location>
            <begin position="5"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="domain" description="Flg_bbr_C" evidence="6">
        <location>
            <begin position="171"/>
            <end position="243"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023143"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU362116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF06429"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABF10610.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="247" mass="25743" checksum="F5092855B1858C20" modified="2006-05-30" version="1">MDRMIYTALSGAKQILDQQAAVSNNLANASTTAFKAQVNMYRAVPVVGQETQTRAFTLASTPGADMKAGPLSYTGRNLDVALQGNGWLAVQMPDGSEAYTRAGSLQVSPDGQLQTSLGLPVMGDGGPIGIPPGSTVSIGTDGTVTARGPGEAANGLAQVGKLRVVTPPPEGLARGDDGYFRLRPGVDPLQQDPNVRVISGALEGSNVNPVESMVEMIANARRFEMQMKMITGADSNDQRANALLSNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="69" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LGY6</accession>
    <name>Q1LGY6_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">VOC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_3718</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10590.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518237.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3718"/>
    <dbReference type="EnsemblBacteria" id="ABF10590">
        <property type="protein sequence ID" value="ABF10590"/>
        <property type="gene ID" value="Rmet_3718"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3718"/>
    <dbReference type="eggNOG" id="COG0346">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046006_12_4_4"/>
    <dbReference type="OMA" id="FSHIAFR"/>
    <dbReference type="OrthoDB" id="1984437at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.180.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029068">
        <property type="entry name" value="Glyas_Bleomycin-R_OHBP_Dase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037523">
        <property type="entry name" value="VOC"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54593">
        <property type="entry name" value="SSF54593"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51819">
        <property type="entry name" value="VOC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="VOC" evidence="1">
        <location>
            <begin position="14"/>
            <end position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF10590.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="154" mass="16703" checksum="335969370E38AC63" modified="2006-05-30" version="1">MEPAHPASPRDAWQFDHLNVSIGADHALRALFEGVMDMHPGYRPPFPFPGLWLYEGDQAMVHAVDDDSLSASSGEVRMGHIAFRSQARATDVIARLQAAGLRFRVAHVPADGTIQIFVPLPGGLVVELDVPGDPDIATSHQYSAERRAPDASDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LGZ5</accession>
    <name>Q1LGZ5_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Protocatechuate 4,5-dioxygenase (LigAB fusion protein)</fullName>
            <ecNumber evidence="3">1.13.11.8</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_3709</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EC" id="1.13.11.8" evidence="3"/>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10581.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518229.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3709"/>
    <dbReference type="EnsemblBacteria" id="ABF10581">
        <property type="protein sequence ID" value="ABF10581"/>
        <property type="gene ID" value="Rmet_3709"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3709"/>
    <dbReference type="eggNOG" id="COG3384">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_653589_0_0_4"/>
    <dbReference type="OMA" id="DPVWAPI"/>
    <dbReference type="OrthoDB" id="446375at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008198">
        <property type="term" value="F:ferrous iron binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018579">
        <property type="term" value="F:protocatechuate 4,5-dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006725">
        <property type="term" value="P:cellular aromatic compound metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07923">
        <property type="entry name" value="Gallate_dioxygenase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd07950">
        <property type="entry name" value="Gallate_Doxase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.700.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034940">
        <property type="entry name" value="Gallate_dioxygenase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034939">
        <property type="entry name" value="Gallate_Doxase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036622">
        <property type="entry name" value="LigA_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011986">
        <property type="entry name" value="Xdiol_dOase_LigA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004183">
        <property type="entry name" value="Xdiol_dOase_suB"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07746">
        <property type="entry name" value="LigA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02900">
        <property type="entry name" value="LigB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48076">
        <property type="entry name" value="SSF48076"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="LigB" evidence="1">
        <location>
            <begin position="7"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="domain" description="LigA" evidence="2">
        <location>
            <begin position="324"/>
            <end position="410"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF10581.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="430" mass="47828" checksum="D6332401FBCD4700" modified="2006-05-30" version="1">MATIIGAIASSHTPTIGFALDAGKQQDPAWAPIFAAYAPVSEWLARKKPDVLLVVYNDHVTSFFFDHYSAFALGIGDTYHAADEGGGPRNLPPARGHVELARHVGASLVADEFDMSFFQDKPLDHGCFSPMSVMLPHEGEWPVKILPLQVGVLQFPAPSARRCYKLGQAIRRAVESYPEDLKVAIVATGGLSHQVHGERAGFNNTAWDHQFLDLFESDPESLLDITHAEYAKRGGFESAEVVMWMVMRGAMSSRVRCLHRDYYLPSMTGIAVAVYENESADAPALREARAERQREHIGHQLHGIEQLDGTYPFTLETSVRAYRINRYLHQLIDPAFRERFRADPLATYDEAALTPEERDMITRRDWRGMIHYGVIFFMLEKLGAVCGVSNLHIYAAMRGQSLEDFQKTRNAPGALYSVAGSGSASWSAKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LH08</accession>
    <name>Q1LH08_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Peptidase M20</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Rmet_3696</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="5">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="5">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10568.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3696"/>
    <dbReference type="EnsemblBacteria" id="ABF10568">
        <property type="protein sequence ID" value="ABF10568"/>
        <property type="gene ID" value="Rmet_3696"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3696"/>
    <dbReference type="eggNOG" id="COG0624">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_051308_0_0_4"/>
    <dbReference type="OMA" id="DDTRGLA"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001261">
        <property type="entry name" value="ArgE/DapE_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036264">
        <property type="entry name" value="Bact_exopeptidase_dim_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002933">
        <property type="entry name" value="Peptidase_M20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011650">
        <property type="entry name" value="Peptidase_M20_dimer"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07687">
        <property type="entry name" value="M20_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01546">
        <property type="entry name" value="Peptidase_M20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55031">
        <property type="entry name" value="SSF55031"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00758">
        <property type="entry name" value="ARGE_DAPE_CPG2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0614">Plasmid</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193119" evidence="2">
        <location>
            <begin position="26"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="domain" description="M20_dimer" evidence="3">
        <location>
            <begin position="226"/>
            <end position="325"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF07687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF10568.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="452" mass="47386" checksum="7087AC13FA7B2CEC" modified="2006-05-30" version="1">MPARCTVVALAAAALAGMLVGIAHAQTLPLPQVSPAVDTAYTKLEASPAYKKLMTDVRADDARALTELKTMTEIPAPPFKERSRAEYFVSRLKALGLADAHIDAEGNAIGIRKGVGNGPKLLISAHLDTVFPEGTDVTVKARDGKLYAPGIGDDTRGLAVLLSWLKVLNDNNVRTAGDLVFVGNVGEEELGNLRGMKAVFREHPDIDGMVGIEPGTGDRVLTQGTGSHRYEVTFRGPGGHSFGAFGLPSAIHAMGRAISKISDVRTPSDPKTTFTVGTVSGGTSVNAIAGDARMAVDIRSNGTPALLETEKHIMDAIAAGVADENRRWAPAKSQEITFQTKLIGDRPAGQTPTDSVIVQSAVRVIRANGKEPPTLRGGSTDANVPMALGIPAIIIGSGGKSGGAHSRDEWFDPTNAWEGAQWGLTTVLGLVGVDGVEAPLLARRRGNPPITR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LH68</accession>
    <name>Q1LH68_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Ferredoxin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">fdx</name>
        <name evidence="2" type="ordered locus">Rmet_3636</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10508.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008646885.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3636"/>
    <dbReference type="EnsemblBacteria" id="ABF10508">
        <property type="protein sequence ID" value="ABF10508"/>
        <property type="gene ID" value="Rmet_3636"/>
    </dbReference>
    <dbReference type="GeneID" id="60826192"/>
    <dbReference type="KEGG" id="rme:Rmet_3636"/>
    <dbReference type="eggNOG" id="COG0633">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_082632_8_0_4"/>
    <dbReference type="OMA" id="RYECGMG"/>
    <dbReference type="OrthoDB" id="1837979at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00207">
        <property type="entry name" value="fer2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036010">
        <property type="entry name" value="2Fe-2S_ferredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001041">
        <property type="entry name" value="2Fe-2S_ferredoxin-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012675">
        <property type="entry name" value="Beta-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00111">
        <property type="entry name" value="Fer2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54292">
        <property type="entry name" value="SSF54292"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="2Fe-2S ferredoxin-type" evidence="1">
        <location>
            <begin position="7"/>
            <end position="86"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF10508.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="100" mass="11453" checksum="D45D21BCF7C1BC05" modified="2006-05-30" version="1">MPKVVFHKNGQVFVDEVKPETNLVVRAGIKQFPYPNLRYECGMGKCSKCACRVLSGAEHLPPPNWKEKKQLGDRLDQGFRLTCQIWLTHDIELEQEELAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LHA2</accession>
    <name>Q1LHA2_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Transcriptional regulator, MerR-family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_3602</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10474.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3602"/>
    <dbReference type="EnsemblBacteria" id="ABF10474">
        <property type="protein sequence ID" value="ABF10474"/>
        <property type="gene ID" value="Rmet_3602"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3602"/>
    <dbReference type="eggNOG" id="COG0789">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_053144_1_1_4"/>
    <dbReference type="OMA" id="EGRIAWY"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009061">
        <property type="entry name" value="DNA-bd_dom_put_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000551">
        <property type="entry name" value="MerR-type_HTH_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13411">
        <property type="entry name" value="MerR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00040">
        <property type="entry name" value="HTHMERR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00422">
        <property type="entry name" value="HTH_MERR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46955">
        <property type="entry name" value="SSF46955"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50937">
        <property type="entry name" value="HTH_MERR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH merR-type" evidence="2">
        <location>
            <begin position="44"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="20"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="20"/>
            <end position="35"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF10474.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="293" mass="31987" checksum="71D81E3110B8BE2E" modified="2006-05-30" version="1">MPHCNVADSVRIFAMVSIKRSGTPTPSSPTDSDSAQAGGKAFPEYTIDELARVAGTTVRNIRSYQDRGLIDPPERRGRVGIYTQAHLGRLRLIHHLLARGYTLANIMELLKAIVEGHDLRSILGLETAISSPWNSEAPRHYSYLALARLFGRAISRTALAKAISLGLLEPDGFGYLARSPRALAAGAEMAKAGFPLEDVLDIIEQARGHFQAVSDRIVGMVVRELDRFGEGKLPPPSEVPRIVDVLWRIRPLALVALETEMMRALEISANKYLGDRVAAILEHLHEEGGSAGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="69" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LHD8</accession>
    <name>Q1LHD8_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">LysM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_3566</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10438.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3566"/>
    <dbReference type="EnsemblBacteria" id="ABF10438">
        <property type="protein sequence ID" value="ABF10438"/>
        <property type="gene ID" value="Rmet_3566"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3566"/>
    <dbReference type="eggNOG" id="COG1652">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_050533_0_0_4"/>
    <dbReference type="OMA" id="PELWGMN"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd00118">
        <property type="entry name" value="LysM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.350.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018392">
        <property type="entry name" value="LysM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036779">
        <property type="entry name" value="LysM_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01476">
        <property type="entry name" value="LysM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00257">
        <property type="entry name" value="LysM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54106">
        <property type="entry name" value="SSF54106"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51782">
        <property type="entry name" value="LYSM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="LysM" evidence="1">
        <location>
            <begin position="37"/>
            <end position="86"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF10438.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="358" mass="38018" checksum="556FC24CC5702051" modified="2006-05-30" version="1">MAVQAADLSVSPVQLAEADRTAQQGIPVADLSPNAPSQYTVRSGDTLWGISGQYLRQPWRWPQLWGMNRQQVHNPHLIYPGQILYLVQRDGRAYLSTNPPGGASGDVRLSPRIRGNGGDGEAIQSIASSDIEPFLIRPLVVDQGTVDTSARIVALPDARVYMGRGDMAYARGIAPADASLGSDWQAFRPASPVLDPVTGRVIGFEAAYEGSVRVTRGPEGPNAVTTVQATQSLQEIGPGSLLLPQPQRELIRYVPHAPDGEVEGRVAAVYGGVRYGGAKQIVVLNIGANAGLEPGHVLALSSDGGHVADPTDGNRAIELPDDRYGLAYVYRVFPGVAYALVTDASNVMKVGDIASTPR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-10-31" modified="2022-05-25" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LHL9</accession>
    <name>GCS2_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Putative glutamate--cysteine ligase 2</fullName>
            <ecNumber evidence="1">6.3.2.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Gamma-glutamylcysteine synthetase 2</fullName>
            <shortName evidence="1">GCS 2</shortName>
            <shortName evidence="1">Gamma-GCS 2</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name type="ordered locus">Rmet_3485</name>
    </gene>
    <organism>
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">ATP-dependent carboxylate-amine ligase which exhibits weak glutamate--cysteine ligase activity.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + L-cysteine + L-glutamate = ADP + gamma-L-glutamyl-L-cysteine + H(+) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:13285"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:35235"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58173"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.2.2"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the glutamate--cysteine ligase type 2 family. YbdK subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.3.2.2" evidence="1"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10357.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517914.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q1LHL9"/>
    <dbReference type="SMR" id="Q1LHL9"/>
    <dbReference type="STRING" id="266264.Rmet_3485"/>
    <dbReference type="EnsemblBacteria" id="ABF10357">
        <property type="protein sequence ID" value="ABF10357"/>
        <property type="gene ID" value="Rmet_3485"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3485"/>
    <dbReference type="eggNOG" id="COG2170">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_044848_1_1_4"/>
    <dbReference type="OMA" id="LIFGLHV"/>
    <dbReference type="OrthoDB" id="991285at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004357">
        <property type="term" value="F:glutamate-cysteine ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042398">
        <property type="term" value="P:cellular modified amino acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01609">
        <property type="entry name" value="Glu_cys_ligase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006336">
        <property type="entry name" value="GCS2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014746">
        <property type="entry name" value="Gln_synth/guanido_kin_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011793">
        <property type="entry name" value="YbdK"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04107">
        <property type="entry name" value="GCS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55931">
        <property type="entry name" value="SSF55931"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02050">
        <property type="entry name" value="gshA_cyan_rel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000255807" description="Putative glutamate--cysteine ligase 2">
        <location>
            <begin position="1"/>
            <end position="372"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01609"/>
        </source>
    </evidence>
    <sequence length="372" mass="41653" checksum="C2167439E6B5A1C6" modified="2006-05-30" version="1">MSLEPFKHSEALTFGVELELQLVNRHDYDLAPFAPDLLRALRGTQHAGDIKPEISPSMIEISTGICHDYAQALEELSVMRDLMVNASRGLNLGICGGGTHPFQVWSDRTISDSPRYQYISELYGYLAKQFTVFGQHVHIGCPSPDESLFLLHAIGRYVPHFIALAASSPYVQGVDTGFASARLNSVAAFPMSGRAPFVLTWDAFKAYFDKMRATGVIESMKDFYWDIRPKPEFGTIEVRVMDTPLTVQRACDIAAYIQALARYLLLSRPFMPQEDDYLVYTFNRFQACRFGLEGEYVHPNELTRMPIADHILSICDALEPHAEALGSLEALSNIRALAASRSNDAHWVRAANRDAHSLRDMVRQTCEQWGGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="72" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LHM9</accession>
    <name>Q1LHM9_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative ABC-type branched-chain amino acid transport system, periplasmic component</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_3475</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10347.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517905.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3475"/>
    <dbReference type="EnsemblBacteria" id="ABF10347">
        <property type="protein sequence ID" value="ABF10347"/>
        <property type="gene ID" value="Rmet_3475"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3475"/>
    <dbReference type="eggNOG" id="COG0683">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027128_7_2_4"/>
    <dbReference type="OMA" id="QGWYGFL"/>
    <dbReference type="OrthoDB" id="776281at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028081">
        <property type="entry name" value="Leu-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028082">
        <property type="entry name" value="Peripla_BP_I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006311">
        <property type="entry name" value="TAT_signal"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13458">
        <property type="entry name" value="Peripla_BP_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53822">
        <property type="entry name" value="SSF53822"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51318">
        <property type="entry name" value="TAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peripla_BP_6" evidence="1">
        <location>
            <begin position="56"/>
            <end position="367"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF10347.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="392" mass="40799" checksum="58DC10B35829202B" modified="2006-05-30" version="1">MRQAPPQSKSSCAPASAGRRRTLGALGSLGALSSLAVIAPQTAFAQPAPSGARRQLVVGQLVDRTGPQADLSRDYLAGAKVLFDAYNASNGSLRIVHVVRDADTNPRGATAQAVALADSEHAEVLFGPGDALLPALAASTELSRRGVQIVAPLSGLSLNAENVWFTRADYQSELDAAVRQLRGFGLTAVTLAVSPDFAAGTLAAGTPWLSRLEKAMSVQTVPLTGNFDESARRIAAGRPGAVIVGGDTLAYGSLGRALAAQNWYGFLVGLSAVSPVSAREILGTGYAGGMVVTQIAPGPQESTLRVVKEHVARMKQYLDEPPSPATLAGYIGAAWLVRAASGIKAPGPVELRRALQTRVDVGDFMLDFTRGLRGSQYVQLAPINKSGLPQRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LHQ6</accession>
    <name>Q1LHQ6_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Glyoxylate carboligase</fullName>
            <ecNumber evidence="6">4.1.1.47</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">gcl</name>
        <name evidence="6" type="ordered locus">Rmet_3448</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the TPP enzyme family.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.47" evidence="6"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10320.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517879.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3448"/>
    <dbReference type="EnsemblBacteria" id="ABF10320">
        <property type="protein sequence ID" value="ABF10320"/>
        <property type="gene ID" value="Rmet_3448"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3448"/>
    <dbReference type="eggNOG" id="COG3960">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013748_1_2_4"/>
    <dbReference type="OMA" id="TLMGWGA"/>
    <dbReference type="OrthoDB" id="391134at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016874">
        <property type="term" value="F:ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009028">
        <property type="term" value="F:tartronate-semialdehyde synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030976">
        <property type="term" value="F:thiamine pyrophosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009436">
        <property type="term" value="P:glyoxylate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029035">
        <property type="entry name" value="DHS-like_NAD/FAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006397">
        <property type="entry name" value="Glyox_carbo_lig"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012000">
        <property type="entry name" value="Thiamin_PyroP_enz_cen_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012001">
        <property type="entry name" value="Thiamin_PyroP_enz_TPP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045229">
        <property type="entry name" value="TPP_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011766">
        <property type="entry name" value="TPP_enzyme-bd_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18968">
        <property type="entry name" value="PTHR18968"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18968:SF14">
        <property type="entry name" value="PTHR18968:SF14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02775">
        <property type="entry name" value="TPP_enzyme_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00205">
        <property type="entry name" value="TPP_enzyme_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02776">
        <property type="entry name" value="TPP_enzyme_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52467">
        <property type="entry name" value="SSF52467"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="SSF52518"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01504">
        <property type="entry name" value="glyox_carbo_lig"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0436">Ligase</keyword>
    <keyword evidence="6" id="KW-0456">Lyase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0786">Thiamine pyrophosphate</keyword>
    <feature type="domain" description="TPP_enzyme_N" evidence="5">
        <location>
            <begin position="4"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="domain" description="TPP_enzyme_M" evidence="3">
        <location>
            <begin position="193"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="domain" description="TPP_enzyme_C" evidence="4">
        <location>
            <begin position="393"/>
            <end position="553"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007812"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU362132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF02775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF02776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABF10320.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="596" mass="65286" checksum="AD0860B6F7703A2C" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LHW9</accession>
    <name>Q1LHW9_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">General secretion pathway protein H</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">gspH</name>
        <name evidence="2" type="ordered locus">Rmet_3385</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10257.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3385"/>
    <dbReference type="EnsemblBacteria" id="ABF10257">
        <property type="protein sequence ID" value="ABF10257"/>
        <property type="gene ID" value="Rmet_3385"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3385"/>
    <dbReference type="eggNOG" id="COG2165">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_113215_0_0_4"/>
    <dbReference type="OMA" id="NPRTDLN"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015627">
        <property type="term" value="C:type II protein secretion system complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015628">
        <property type="term" value="P:protein secretion by the type II secretion system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012902">
        <property type="entry name" value="N_methyl_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045584">
        <property type="entry name" value="Pilin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002416">
        <property type="entry name" value="T2SS_protein-GspH"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07963">
        <property type="entry name" value="N_methyl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54523">
        <property type="entry name" value="SSF54523"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02532">
        <property type="entry name" value="IV_pilin_GFxxxE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01708">
        <property type="entry name" value="typeII_sec_gspH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="26"/>
            <end position="46"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF10257.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="174" mass="18943" checksum="05639AA9DB9EBAE0" modified="2006-05-30" version="1">MRPATPRGLTAFAPCRRTCRRVPGFTLLELLVVMVIAGIVISLVAVNASPNERGRVLDDGQRIARLFELAQEDAQLGARPIAWEGDAGGWRFLESTPQGWVPMRTDIFAPGQWRVRLDGAVIAEGGRRLGTGPVRLVFGRELIDEPQRLILTSGAIRVDVTGDGSGRYVATVLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LID7</accession>
    <name>Q1LID7_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative transcriptional regulator, HxlR-type</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_3217</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10089.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517699.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3217"/>
    <dbReference type="EnsemblBacteria" id="ABF10089">
        <property type="protein sequence ID" value="ABF10089"/>
        <property type="gene ID" value="Rmet_3217"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3217"/>
    <dbReference type="eggNOG" id="COG1733">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_111585_0_0_4"/>
    <dbReference type="OMA" id="QECSIAR"/>
    <dbReference type="OrthoDB" id="1919329at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002577">
        <property type="entry name" value="HTH_HxlR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01638">
        <property type="entry name" value="HxlR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51118">
        <property type="entry name" value="HTH_HXLR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH hxlR-type" evidence="1">
        <location>
            <begin position="11"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF10089.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="170" mass="18831" checksum="9DBEAE9247A5825E" modified="2006-05-30" version="1">MIPTDFSTMPCPIARSMAMLGERWAILLMREAYYGSLRFDEFEKNLGIAPNILSARLKTMVEHGLLDKVPAPGGGARHVYVLTEMGRDFFPVFVTLKDWADRWLADPKGPLTVLEDKREGKEIIAGKLTRDDGSAITVDDLRVKPGPGAGRYLRARFGEPSSEAADHAKV</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-01-15" modified="2022-05-25" version="96" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LIM3</accession>
    <name>MRAY_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Phospho-N-acetylmuramoyl-pentapeptide-transferase</fullName>
            <ecNumber evidence="1">2.7.8.13</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">UDP-MurNAc-pentapeptide phosphotransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">mraY</name>
        <name type="ordered locus">Rmet_3131</name>
    </gene>
    <organism>
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the initial step of the lipid cycle reactions in the biosynthesis of the cell wall peptidoglycan: transfers peptidoglycan precursor phospho-MurNAc-pentapeptide from UDP-MurNAc-pentapeptide onto the lipid carrier undecaprenyl phosphate, yielding undecaprenyl-pyrophosphoryl-MurNAc-pentapeptide, known as lipid I.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>di-trans,octa-cis-undecaprenyl phosphate + UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-gamma-D-glutamyl-meso-2,6-diaminopimeloyl-D-alanyl-D-alanine = di-trans-octa-cis-undecaprenyl diphospho-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamyl-meso-2,6-diaminopimeloyl-D-alanyl-D-alanine + UMP</text>
            <dbReference type="Rhea" id="RHEA:28386"/>
            <dbReference type="ChEBI" id="CHEBI:57865"/>
            <dbReference type="ChEBI" id="CHEBI:60392"/>
            <dbReference type="ChEBI" id="CHEBI:61386"/>
            <dbReference type="ChEBI" id="CHEBI:61387"/>
            <dbReference type="EC" id="2.7.8.13"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cell wall biogenesis; peptidoglycan biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the glycosyltransferase 4 family. MraY subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.8.13" evidence="1"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10003.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008650301.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q1LIM3"/>
    <dbReference type="SMR" id="Q1LIM3"/>
    <dbReference type="STRING" id="266264.Rmet_3131"/>
    <dbReference type="EnsemblBacteria" id="ABF10003">
        <property type="protein sequence ID" value="ABF10003"/>
        <property type="gene ID" value="Rmet_3131"/>
    </dbReference>
    <dbReference type="GeneID" id="60820493"/>
    <dbReference type="KEGG" id="rme:Rmet_3131"/>
    <dbReference type="eggNOG" id="COG0472">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023982_0_0_4"/>
    <dbReference type="OMA" id="LMSPLHH"/>
    <dbReference type="OrthoDB" id="1151822at2"/>
    <dbReference type="UniPathway" id="UPA00219"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008963">
        <property type="term" value="F:phospho-N-acetylmuramoyl-pentapeptide-transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051992">
        <property type="term" value="F:UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-meso-2,6-diaminopimelyl-D-alanyl-D-alanine:undecaprenyl-phosphate transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007049">
        <property type="term" value="P:cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009252">
        <property type="term" value="P:peptidoglycan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008360">
        <property type="term" value="P:regulation of cell shape"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06852">
        <property type="entry name" value="GT_MraY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00038">
        <property type="entry name" value="MraY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000715">
        <property type="entry name" value="Glycosyl_transferase_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003524">
        <property type="entry name" value="PNAcMuramoyl-5peptid_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018480">
        <property type="entry name" value="PNAcMuramoyl-5peptid_Trfase_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22926">
        <property type="entry name" value="PTHR22926"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00953">
        <property type="entry name" value="Glycos_transf_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10555">
        <property type="entry name" value="MraY_sig1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00445">
        <property type="entry name" value="mraY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01347">
        <property type="entry name" value="MRAY_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01348">
        <property type="entry name" value="MRAY_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0131">Cell cycle</keyword>
    <keyword id="KW-0132">Cell division</keyword>
    <keyword id="KW-0997">Cell inner membrane</keyword>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0133">Cell shape</keyword>
    <keyword id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0573">Peptidoglycan synthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_1000003036" description="Phospho-N-acetylmuramoyl-pentapeptide-transferase">
        <location>
            <begin position="1"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="25"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="74"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="97"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="134"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="167"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="190"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="222"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="259"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="286"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="311"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="366"/>
            <end position="386"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00038"/>
        </source>
    </evidence>
    <sequence length="389" mass="43060" checksum="64511404F45A253C" modified="2006-05-30" version="1">MLLTLAQWLQNDYSFLRVVNYLTFRAVMANVTALVIGLGFGPWVIRRLTELKIGQAVRTIGPQTHLVKAGTPTMGGVLVLISIAISTLLWCDWGNRFIWVVLLVTLGYGAIGWVDDYRKVVYRDPRGMSSREKFFWQTLIGLVAAVYLAFSVSEASNVRIWSLFLSWIEGGMFADVPYKMNLIVPFFKEVSYPLGVTGFIVLTYLVIVGSSNAVNLTDGLDGLVIMPVVLVGGGLGVFAYVMGNAVYSKYLLFPHIPGAGELLIFCSAMAGAGLAFLWFNAHPARVFMGDVGALALGGALGTVAVIVRQEIVLFVMGGIFVVETLSVMLQVSWFKFTKRRYGEGRRLFRMAPLHHHFELGGWKETQVTVRFWIITMLLVLIGLSSLKLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LIQ5</accession>
    <name>Q1LIQ5_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Putative lytic transglycosylase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Rmet_3099</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09971.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517599.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3099"/>
    <dbReference type="CAZy" id="GH23">
        <property type="family name" value="Glycoside Hydrolase Family 23"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF09971">
        <property type="protein sequence ID" value="ABF09971"/>
        <property type="gene ID" value="Rmet_3099"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3099"/>
    <dbReference type="eggNOG" id="COG0741">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_088197_0_0_4"/>
    <dbReference type="OMA" id="GYMQVMP"/>
    <dbReference type="OrthoDB" id="1444566at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023346">
        <property type="entry name" value="Lysozyme-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008258">
        <property type="entry name" value="Transglycosylase_SLT_dom_1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01464">
        <property type="entry name" value="SLT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53955">
        <property type="entry name" value="SSF53955"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193495" evidence="2">
        <location>
            <begin position="31"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="domain" description="SLT" evidence="3">
        <location>
            <begin position="100"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="216"/>
            <end position="251"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF09971.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="251" mass="27793" checksum="85761162B49F573A" modified="2006-05-30" version="1">MRIPDLPTRNRWSAWAGGLLCATLIANAHAGAQKEEALADSVRGALAAAIADNRPVRPAFASGSERLGYLKWLGEMSRRLEARIPEPQVRVELIETVYYESKRAGLEPSLVLGLVQVESNFRKYAISSADARGLMQVMPFWVRSIGDGDTRKLFHLQSNLRYGCTILRHYLDRENGDLFLALGRYNGSRGRPEYPNAVLAAWKRWQYSEATVTIAGDPDPAAVTPAPVRRAVPPESPARNPFSPQRLANPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="62" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LIT3</accession>
    <name>Q1LIT3_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Histone H1-like protein HC2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Rmet_3071</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Might have a role in establishing the nucleoid structure of elementary bodies.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the histone H1/H5 family. HCT subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09943.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517571.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF09943">
        <property type="protein sequence ID" value="ABF09943"/>
        <property type="gene ID" value="Rmet_3071"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3071"/>
    <dbReference type="eggNOG" id="ENOG5031DFK">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_115743_0_0_4"/>
    <dbReference type="OMA" id="FPTGNRP"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030527">
        <property type="term" value="F:structural constituent of chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030261">
        <property type="term" value="P:chromosome condensation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009970">
        <property type="entry name" value="HC2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07382">
        <property type="entry name" value="HC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="95"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="9"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="95"/>
            <end position="163"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002344"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008424"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF09943.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="201" mass="19679" checksum="09D8458C4F787170" modified="2006-05-30" version="1">MATAAKKKPAAKKAAKPAAKKAAVKKVAAKKAAPAAKKAAAKKVATKKVAAKKVATKKVATKKVAAKKAAPAKKAAVKKVAAKKVATKKVAAKKAAPAKKAAVKKVAAKKPAAKKVVAKKAAPAKKAAAKKVAAKKPAAKKAAAKKPAAKKPAAKKAAAKKPAAKKAAAKPAAAPAVAPAAAAKTALNPAAAWPFPTGNRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="92" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LJ10</accession>
    <name>Q1LJ10_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Phosphite transport system permease protein PtxC</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">ptxC</name>
        <name evidence="6" type="ordered locus">Rmet_2993</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cell membrane</location>
            <topology evidence="4">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the binding-protein-dependent transport system permease family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09866.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_003056093.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2993"/>
    <dbReference type="EnsemblBacteria" id="ABF09866">
        <property type="protein sequence ID" value="ABF09866"/>
        <property type="gene ID" value="Rmet_2993"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2993"/>
    <dbReference type="eggNOG" id="COG3639">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_064254_1_1_4"/>
    <dbReference type="OMA" id="SMRMFAG"/>
    <dbReference type="OrthoDB" id="1209768at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005887">
        <property type="term" value="C:integral component of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015416">
        <property type="term" value="F:ABC-type phosphonate transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005769">
        <property type="entry name" value="PhnE/PtxC"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="SSF161098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01097">
        <property type="entry name" value="PhnE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2 4" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="20"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="79"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="127"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="214"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="241"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="5">
        <location>
            <begin position="75"/>
            <end position="258"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABF09866.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="266" mass="28642" checksum="251F4E91C035A5EC" modified="2006-05-30" version="1">MTSAAFENIISRDKREQFIGLRNTAFVLLVCLVALYVTGFFDAQRFIEGAPAVKQLSSEMVPPNFDRWEHWVIPLRDTLAMSIAGTALTIVLSLPLALLAAPNTTPNPLVGRVARVILAAFRSVPEIILGILFVAAVGFGALPGVLALALHSTGMVAKFYAEAIEHVDPKPLEAAAAVGASRFQVISHAVIPQVLPQLADITIYRWEYHFRASAVLGIVGAGGIGFELMAALRLVKYDEVSAILLSILACVLVVDSIGSMLRKRLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LJP0</accession>
    <name>Q1LJP0_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Formate dehydrogenase-specific chaperone</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">torD</name>
        <name evidence="1" type="ordered locus">Rmet_2763</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09636.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517334.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2763"/>
    <dbReference type="EnsemblBacteria" id="ABF09636">
        <property type="protein sequence ID" value="ABF09636"/>
        <property type="gene ID" value="Rmet_2763"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2763"/>
    <dbReference type="eggNOG" id="COG3381">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_077650_2_0_4"/>
    <dbReference type="OMA" id="QRHMGSW"/>
    <dbReference type="OrthoDB" id="1995553at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020945">
        <property type="entry name" value="DMSO/NO3_reduct_chaperone"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036411">
        <property type="entry name" value="TorD-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02613">
        <property type="entry name" value="Nitrate_red_del"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89155">
        <property type="entry name" value="SSF89155"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF09636.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="217" mass="24034" checksum="D18313BFD2C06F2C" modified="2006-05-30" version="1">MHHDMSDFQPIQLTAQTPAHGEPDPEDTARADLYGLLATLFYQAPDATLLHHIAANRASGADADTVLGRAWNTLCDAASITTAVEAADEYAQLFVGVGKQEIFLYGSFYQAGFLNERPLVALRDDLARYGLTRHDNVSETEDHVAALCEVMRYLVAGDDLAVSNLGEQQRFFARHLQPWVDRLCDALENHPRARFYRSAAGLLRAFCSVEAVALEMH</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="56" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LJV0</accession>
    <name>Q1LJV0_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF5594 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_2703</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09576.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517274.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF09576">
        <property type="protein sequence ID" value="ABF09576"/>
        <property type="gene ID" value="Rmet_2703"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2703"/>
    <dbReference type="eggNOG" id="ENOG5033X0X">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1977367_0_0_4"/>
    <dbReference type="OMA" id="TWDSDEI"/>
    <dbReference type="OrthoDB" id="1717357at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040953">
        <property type="entry name" value="DUF5594"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18057">
        <property type="entry name" value="DUF5594"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF5594" evidence="1">
        <location>
            <begin position="6"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF18057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF09576.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="129" mass="14467" checksum="BA22F45DFD9FCE4D" modified="2006-05-30" version="1">MHPIPAHRFNAEFLPRIAERVQSIFHGTLNVELVPHTSADQPARLRIWAVARAGGGRYACPLDITLSWDSYEVDRLFASGGDQRFAEYLGALPGKLNAWQDARQIDFNSRTQADPTVLLGGLDFEHLLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="114" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LJW0</accession>
    <name>Q1LJW0_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7 10">3,4-dihydroxy-2-butanone 4-phosphate synthase</fullName>
            <shortName evidence="10">DHBP synthase</shortName>
            <ecNumber evidence="6 10">4.1.99.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">ribBA</name>
        <name evidence="10" type="synonym">ribB</name>
        <name evidence="12" type="ordered locus">Rmet_2693</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2 10">Catalyzes the conversion of D-ribulose 5-phosphate to formate and 3,4-dihydroxy-2-butanone 4-phosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 10">
            <text>D-ribulose 5-phosphate = (2S)-2-hydroxy-3-oxobutyl phosphate + formate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:18457"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15740"/>
            <dbReference type="ChEBI" id="CHEBI:58121"/>
            <dbReference type="ChEBI" id="CHEBI:58830"/>
            <dbReference type="EC" id="4.1.99.12"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <cofactor evidence="10">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="10">Binds 2 divalent metal cations per subunit. Magnesium or manganese.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3 10">Cofactor biosynthesis; riboflavin biosynthesis; 2-hydroxy-3-oxobutyl phosphate from D-ribulose 5-phosphate: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the DHBP synthase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">In the C-terminal section; belongs to the GTP cyclohydrolase II family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">In the N-terminal section; belongs to the DHBP synthase family.</text>
    </comment>
    <dbReference type="EC" id="4.1.99.12" evidence="6 10"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09566.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517266.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2693"/>
    <dbReference type="EnsemblBacteria" id="ABF09566">
        <property type="protein sequence ID" value="ABF09566"/>
        <property type="gene ID" value="Rmet_2693"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2693"/>
    <dbReference type="eggNOG" id="COG0108">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG0807">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020273_1_2_4"/>
    <dbReference type="OMA" id="CTANLMS"/>
    <dbReference type="OrthoDB" id="900513at2"/>
    <dbReference type="UniPathway" id="UPA00275">
        <property type="reaction ID" value="UER00399"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008686">
        <property type="term" value="F:3,4-dihydroxy-2-butanone-4-phosphate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030145">
        <property type="term" value="F:manganese ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009231">
        <property type="term" value="P:riboflavin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10990">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00180">
        <property type="entry name" value="RibB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017945">
        <property type="entry name" value="DHBP_synth_RibB-like_a/b_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000422">
        <property type="entry name" value="DHBP_synthase_RibB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032677">
        <property type="entry name" value="GTP_cyclohydro_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036144">
        <property type="entry name" value="RibA-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00926">
        <property type="entry name" value="DHBP_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00925">
        <property type="entry name" value="GTP_cyclohydro2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF142695">
        <property type="entry name" value="SSF142695"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55821">
        <property type="entry name" value="SSF55821"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00506">
        <property type="entry name" value="ribB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-0456">Lyase</keyword>
    <keyword evidence="10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="10" id="KW-0464">Manganese</keyword>
    <keyword evidence="9 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 10" id="KW-0686">Riboflavin biosynthesis</keyword>
    <feature type="domain" description="GTP_cyclohydro2" evidence="11">
        <location>
            <begin position="208"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="region of interest" description="D-ribulose 5-phosphate binding" evidence="10">
        <location>
            <begin position="27"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="region of interest" description="D-ribulose 5-phosphate binding" evidence="10">
        <location>
            <begin position="140"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="28"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="32"/>
        </location>
        <ligand>
            <name>D-ribulose 5-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58121"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="143"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="site" description="Essential for catalytic activity" evidence="10">
        <location>
            <position position="126"/>
        </location>
    </feature>
    <feature type="site" description="Essential for catalytic activity" evidence="10">
        <location>
            <position position="164"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004904"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00008976"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012153"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00018836"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022619"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00925"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ABF09566.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="371" mass="40415" checksum="AB8BBE541A96A452" modified="2006-05-30" version="1">MTISRIEDIIADIRAGRMVILVDEEDRENEGDLVLAADFVTPEAINFMAKYGRGLICLTLTAERCRQLELYPMVSRNGTVHGTNFTVSIEAAEGVTTGISAADRSRTVQAAVARDARASDLVQPGHIFPLTAQPGGVLIRAGHTEAGCDLGTLAGLTPAAVICEIMKDDGTMARLPDLIEFAREHDLKIGTIADLIHYRSRTESIIERVGERDMQTPWGTFHSVLYRDKPSGRAHVALVKGKPQPDRETLVRVHEPLSVLDLLEAGHTTHSWSLPAALEVLHGAEHGVMVLLNCGDSPEMLFEQFAALDAPENRPRRKPDLRTYGVGAQILRDIGVGKMRVLGSPQKMPSMTGYELEIVGCQPMSGESNRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LK06</accession>
    <name>Q1LK06_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Cyclopropane-fatty-acyl-phospholipid synthase</fullName>
            <ecNumber evidence="3">2.1.1.79</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">cfa</name>
        <name evidence="3" type="ordered locus">Rmet_2643</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CFA/CMAS family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.79" evidence="3"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09520.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008646259.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2643"/>
    <dbReference type="EnsemblBacteria" id="ABF09520">
        <property type="protein sequence ID" value="ABF09520"/>
        <property type="gene ID" value="Rmet_2643"/>
    </dbReference>
    <dbReference type="GeneID" id="60820921"/>
    <dbReference type="KEGG" id="rme:Rmet_2643"/>
    <dbReference type="eggNOG" id="COG2230">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026434_6_2_4"/>
    <dbReference type="OMA" id="HRVEGIP"/>
    <dbReference type="OrthoDB" id="1518440at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008825">
        <property type="term" value="F:cyclopropane-fatty-acyl-phospholipid synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008610">
        <property type="term" value="P:lipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003333">
        <property type="entry name" value="Mycolic_cyclopropane_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF003085">
        <property type="entry name" value="CMAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="active site" evidence="2">
        <location>
            <position position="371"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010815"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR003085-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF09520.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="413" mass="46378" checksum="D6E561943B00B4D4" modified="2006-05-30" version="1">MFFKQTLDKQLDSWIADVREHSNLPVKLRLWNGSEYQLGSFDRPAVTLTVREASALPFLLVPSLDNLSEAYVQEKIDLDGRLADIIKVGYGLSAAAARRAGGVLNKVAQHFTHTKAEDKASIQYHYDVSNDFYKLWLDPNMVYSCAYFENGNEDLATAQIKKIDHILTKIRLEPGQTLLDIGCGWGALVLRAAQKFGARCVGITLSQNQFDLATARVRAAGLQDRIEIRLQDYRDISGQFDRITSVGMFEHVGKDNLPGYFARMRDLLAEGGVAMNHGITATDPDSGEIPMDGSGFMDRYVFPQGELPHIGMVLTTMQKGGLEAFDVELLRRHYAQTLRHWAENFETNADTIREMVGEKKFRIWRIYLAGCSHAFTSGKMSIYQVVCQKADRNADTFPTSRRYIYASPIGTPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LK29</accession>
    <name>Q1LK29_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Sulfurtransferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">sseA</name>
        <name evidence="4" type="ordered locus">Rmet_2620</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09497.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517198.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2620"/>
    <dbReference type="EnsemblBacteria" id="ABF09497">
        <property type="protein sequence ID" value="ABF09497"/>
        <property type="gene ID" value="Rmet_2620"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2620"/>
    <dbReference type="eggNOG" id="COG2897">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031618_0_0_4"/>
    <dbReference type="OMA" id="QRPGHVP"/>
    <dbReference type="OrthoDB" id="920668at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004792">
        <property type="term" value="F:thiosulfate sulfurtransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.250.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001763">
        <property type="entry name" value="Rhodanese-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036873">
        <property type="entry name" value="Rhodanese-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001307">
        <property type="entry name" value="Thiosulphate_STrfase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045078">
        <property type="entry name" value="TST/MPST-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11364">
        <property type="entry name" value="PTHR11364"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00581">
        <property type="entry name" value="Rhodanese"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00450">
        <property type="entry name" value="RHOD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52821">
        <property type="entry name" value="SSF52821"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00380">
        <property type="entry name" value="RHODANESE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00683">
        <property type="entry name" value="RHODANESE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50206">
        <property type="entry name" value="RHODANESE_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Rhodanese" evidence="3">
        <location>
            <begin position="18"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="domain" description="Rhodanese" evidence="3">
        <location>
            <begin position="169"/>
            <end position="282"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU000507"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF09497.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="285" mass="30289" checksum="6AEE63065430C511" modified="2006-05-30" version="1">MPTPLITATELDQLRAAGKPRVVVFDCSFDLTNPAAGRDSYQQGHIPGAYYLHLDNELSGPKTGLSGRHPLPDAELFAARLRVLGVDDDTLVVAYDGQGSMYAARLWWMLRWIGHAAALVLDGGRNAWVAAGLPLEHGATAEPEIGGNLMRRASLVKTVDADALVANLSQPSLLVVDARAADRFRGENETLDPVGGHIPGAANRFFKDNLTADGYFKPADQLRADFGQVFTGKAPAQTVMQCGSGVTACHNLLALEVAGLTGAALYPGSWSEWCADPKRPVATGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="53" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LKB0</accession>
    <name>Q1LKB0_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_2539</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09416.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517116.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2539"/>
    <dbReference type="EnsemblBacteria" id="ABF09416">
        <property type="protein sequence ID" value="ABF09416"/>
        <property type="gene ID" value="Rmet_2539"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2539"/>
    <dbReference type="eggNOG" id="ENOG5030717">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_083007_1_0_4"/>
    <dbReference type="OMA" id="HPYAQCP"/>
    <dbReference type="OrthoDB" id="1739479at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021312">
        <property type="entry name" value="DUF2889"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11136">
        <property type="entry name" value="DUF2889"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF09416.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="190" mass="21149" checksum="276A9DDC0DD25E4D" modified="2006-05-30" version="1">MEPETPNQPERTLRHTRQILCRGYLRDDGLYDIEAELHDISPVPTELPFIALPAGGSIHQMRLTMTIDAELVIRHMSARTDTGPTPYCAEINAAYAVLEGLRVGSGFRQEAKARLGGARGCTHLTELLGPLATTAMQTIMSMQRETTPWHARLEGDAPILQPWVLGTCHAYRLDGEAAKVIWPIDRRARK</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="101" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LKM0</accession>
    <name>Q1LKM0_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 5">Malonyl CoA-acyl carrier protein transacylase</fullName>
            <ecNumber evidence="2 5">2.3.1.39</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">fabD</name>
        <name evidence="8" type="ordered locus">Rmet_2429</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 5">
            <text>holo-[ACP] + malonyl-CoA = CoA + malonyl-[ACP]</text>
            <dbReference type="Rhea" id="RHEA:41792"/>
            <dbReference type="Rhea" id="RHEA-COMP:9623"/>
            <dbReference type="Rhea" id="RHEA-COMP:9685"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57384"/>
            <dbReference type="ChEBI" id="CHEBI:64479"/>
            <dbReference type="ChEBI" id="CHEBI:78449"/>
            <dbReference type="EC" id="2.3.1.39"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the fabD family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.39" evidence="2 5"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09306.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517023.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2429"/>
    <dbReference type="EnsemblBacteria" id="ABF09306">
        <property type="protein sequence ID" value="ABF09306"/>
        <property type="gene ID" value="Rmet_2429"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2429"/>
    <dbReference type="eggNOG" id="COG0331">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030558_0_1_4"/>
    <dbReference type="OMA" id="AANYNCP"/>
    <dbReference type="OrthoDB" id="1123553at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004314">
        <property type="term" value="F:[acyl-carrier-protein] S-malonyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.366.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001227">
        <property type="entry name" value="Ac_transferase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014043">
        <property type="entry name" value="Acyl_transferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016035">
        <property type="entry name" value="Acyl_Trfase/lysoPLipase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024925">
        <property type="entry name" value="Malonyl_CoA-ACP_transAc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004410">
        <property type="entry name" value="Malonyl_CoA-ACP_transAc_FabD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016036">
        <property type="entry name" value="Malonyl_transacylase_ACP-bd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00698">
        <property type="entry name" value="Acyl_transf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000446">
        <property type="entry name" value="Mct"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00827">
        <property type="entry name" value="PKS_AT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52151">
        <property type="entry name" value="SSF52151"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55048">
        <property type="entry name" value="SSF55048"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00128">
        <property type="entry name" value="fabD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5 8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PKS_AT" evidence="7">
        <location>
            <begin position="6"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="active site" evidence="6">
        <location>
            <position position="90"/>
        </location>
    </feature>
    <feature type="active site" evidence="6">
        <location>
            <position position="201"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013258"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00018953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRNR" id="PIRNR000446"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR000446-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ABF09306.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="311" mass="31772" checksum="87E5A951867F1A10" modified="2006-05-30" version="1">MKFAFVFPGQGSQSVGMLNAFADNPVVRATLEEASTALGQDIGKLIAEGPAEELNLTTNTQPVMLTAAVAVYRAWLDAGGPAPALVAGHSLGEYSALVAAGVIPFAEAVPLVRFRAQAMQEAVPVGEGGMAAILGLSDDDVRAACAEAAAAGAGVVEAVNFNAPSQVVIAGNKGAVEKACEIAKAKGAKRALPLPVSAPFHSSLLKPASDRLRERMAGLSFSAPAIPLVNNVDVAIISDPAQIKDALVRQAAAPVRWVECVQKIAAEGVTHVIECGPGKVLAGMTKRIDGNLVGGAIFDPASLQETLALLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="108" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LKM3</accession>
    <name>Q1LKM3_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 10">3-oxoacyl-[acyl-carrier-protein] synthase 2</fullName>
            <ecNumber evidence="3 10">2.3.1.179</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">fabF</name>
        <name evidence="14" type="ordered locus">Rmet_2426</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Involved in the type II fatty acid elongation cycle. Catalyzes the elongation of a wide range of acyl-ACP by the addition of two carbons from malonyl-ACP to an acyl acceptor. Can efficiently catalyze the conversion of palmitoleoyl-ACP (cis-hexadec-9-enoyl-ACP) to cis-vaccenoyl-ACP (cis-octadec-11-enoyl-ACP), an essential step in the thermal regulation of fatty acid composition.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>(9Z)-hexadecenoyl-[ACP] + H(+) + malonyl-[ACP] = 3-oxo-(11Z)-octadecenoyl-[ACP] + CO2 + holo-[ACP]</text>
            <dbReference type="Rhea" id="RHEA:55040"/>
            <dbReference type="Rhea" id="RHEA-COMP:9623"/>
            <dbReference type="Rhea" id="RHEA-COMP:9685"/>
            <dbReference type="Rhea" id="RHEA-COMP:10800"/>
            <dbReference type="Rhea" id="RHEA-COMP:14074"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:64479"/>
            <dbReference type="ChEBI" id="CHEBI:78449"/>
            <dbReference type="ChEBI" id="CHEBI:83989"/>
            <dbReference type="ChEBI" id="CHEBI:138538"/>
            <dbReference type="EC" id="2.3.1.179"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>a fatty acyl-[ACP] + H(+) + malonyl-[ACP] = a 3-oxoacyl-[ACP] + CO2 + holo-[ACP]</text>
            <dbReference type="Rhea" id="RHEA:22836"/>
            <dbReference type="Rhea" id="RHEA-COMP:9623"/>
            <dbReference type="Rhea" id="RHEA-COMP:9685"/>
            <dbReference type="Rhea" id="RHEA-COMP:9916"/>
            <dbReference type="Rhea" id="RHEA-COMP:14125"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:64479"/>
            <dbReference type="ChEBI" id="CHEBI:78449"/>
            <dbReference type="ChEBI" id="CHEBI:78776"/>
            <dbReference type="ChEBI" id="CHEBI:138651"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 10">Lipid metabolism; fatty acid biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 10 12">Belongs to the thiolase-like superfamily. Beta-ketoacyl-ACP synthases family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.179" evidence="3 10"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09303.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517021.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2426"/>
    <dbReference type="EnsemblBacteria" id="ABF09303">
        <property type="protein sequence ID" value="ABF09303"/>
        <property type="gene ID" value="Rmet_2426"/>
    </dbReference>
    <dbReference type="GeneID" id="60821100"/>
    <dbReference type="KEGG" id="rme:Rmet_2426"/>
    <dbReference type="eggNOG" id="COG0304">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000022_69_2_4"/>
    <dbReference type="OMA" id="QIGHCLG"/>
    <dbReference type="OrthoDB" id="606297at2"/>
    <dbReference type="UniPathway" id="UPA00094"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004315">
        <property type="term" value="F:3-oxoacyl-[acyl-carrier-protein] synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006633">
        <property type="term" value="P:fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00834">
        <property type="entry name" value="KAS_I_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.47.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017568">
        <property type="entry name" value="3-oxoacyl-ACP_synth-2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000794">
        <property type="entry name" value="Beta-ketoacyl_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018201">
        <property type="entry name" value="Ketoacyl_synth_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014031">
        <property type="entry name" value="Ketoacyl_synth_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014030">
        <property type="entry name" value="Ketoacyl_synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020841">
        <property type="entry name" value="PKS_Beta-ketoAc_synthase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016039">
        <property type="entry name" value="Thiolase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11712">
        <property type="entry name" value="PTHR11712"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00109">
        <property type="entry name" value="ketoacyl-synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02801">
        <property type="entry name" value="Ketoacyl-synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000447">
        <property type="entry name" value="KAS_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00825">
        <property type="entry name" value="PKS_KS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53901">
        <property type="entry name" value="SSF53901"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03150">
        <property type="entry name" value="fabF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00606">
        <property type="entry name" value="B_KETOACYL_SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 10 14" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="8 10" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="6" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="5 10" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="7" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10 12 14" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PKS_KS" evidence="13">
        <location>
            <begin position="6"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="active site" evidence="11">
        <location>
            <position position="164"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012356"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00014657"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRNR" id="PIRNR000447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR000447-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU003694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM00825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ABF09303.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="410" mass="42930" checksum="659F3531E7F0F886" modified="2006-05-30" version="1">MSRRRVVVTGLGLVSPVGNTVAEGWANLVAGKSGIATITKFDHSPFAVHFAGEVKGFNAEDYIPAKEARNMDTFIHFGIAAGSQALKDSGLEVTEANAERIGVLVGSGIGGLPMIEDTQMTLAERGPRRISPFFVPGSIINMISGHLSIIHGLKGPNLAAVTACTTGLHSIGLAARLIQAGDCDAMLAGGAESTVSPLGIGGFAAARALSTRNDDPAAASRPWDKDRDGFVLGEGAGVMMLEEYESAKARGARIYAELIGFGMSGDAFHMTAPNTDGPRRCMVAAIKDAGINPDEVQYLNAHGTSTPLGDKNETDAVKLAFGDHAYKMVVNSTKSMTGHLLGGAGGLESVFTVLAIHNQISPPTINIFNQDPECDLDYVANTARDMKIDVAVKNNFGFGGTNGTLVFRRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LKQ2</accession>
    <name>Q1LKQ2_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Probable membrane transporter protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Rmet_2397</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cell membrane</location>
            <topology evidence="5">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the 4-toluene sulfonate uptake permease (TSUP) (TC 2.A.102) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09274.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004350669.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2397"/>
    <dbReference type="EnsemblBacteria" id="ABF09274">
        <property type="protein sequence ID" value="ABF09274"/>
        <property type="gene ID" value="Rmet_2397"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2397"/>
    <dbReference type="eggNOG" id="COG0730">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045498_5_0_4"/>
    <dbReference type="OMA" id="NLPMKVT"/>
    <dbReference type="OrthoDB" id="881796at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002781">
        <property type="entry name" value="TM_pro_TauE-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01925">
        <property type="entry name" value="TauE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="12"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="43"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="72"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="98"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="154"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="191"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="217"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="250"/>
            <end position="267"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU363041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABF09274.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="268" mass="27833" checksum="7A0AE182A1F65FB6" modified="2006-05-30" version="1">MLSLTGGVLGLIIGAVLGLTGAGGGIFAVPALVFGLGMDIRQAAPVALLAVGAAATLGALQGLRQGVVRYKAAMMLAAAGAVTAPLGVQFAHWLSPRWLNLIFVAIMLVVAYRMFMSSRGSQTQDDLADEPARVCKISKDTGRFVWNVRTATTLGSIGIVSGLATGMLGVGGGFIIVPALAHFSELRMHSIVATSLMVIALLSAVTVFIAWSHGMTLTAPAWTFVLTALVGMSLGRVLARRIPSKMLQRVFSITCVAVAALMLMRNVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LL30</accession>
    <name>Q1LL30_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Flagellar hook-associated protein 2</fullName>
            <shortName evidence="5">HAP2</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Flagellar cap protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">fliD1</name>
        <name evidence="8" type="ordered locus">Rmet_2267</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Required for morphogenesis and for the elongation of the flagellar filament by facilitating polymerization of the flagellin monomers at the tip of growing filament. Forms a capping structure, which prevents flagellin subunits (transported through the central channel of the flagellum) from leaking out without polymerization at the distal end.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2 5">Homopentamer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Secreted</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Bacterial flagellum</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the FliD family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09146.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516972.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2267"/>
    <dbReference type="EnsemblBacteria" id="ABF09146">
        <property type="protein sequence ID" value="ABF09146"/>
        <property type="gene ID" value="Rmet_2267"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2267"/>
    <dbReference type="eggNOG" id="COG1345">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015182_6_1_4"/>
    <dbReference type="OMA" id="IMAERFA"/>
    <dbReference type="OrthoDB" id="883503at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009421">
        <property type="term" value="C:bacterial-type flagellum filament cap"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009424">
        <property type="term" value="C:bacterial-type flagellum hook"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007155">
        <property type="term" value="P:cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040026">
        <property type="entry name" value="FliD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010809">
        <property type="entry name" value="FliD_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003481">
        <property type="entry name" value="FliD_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30288">
        <property type="entry name" value="PTHR30288"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07195">
        <property type="entry name" value="FliD_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02465">
        <property type="entry name" value="FliD_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0975">Bacterial flagellum</keyword>
    <keyword evidence="8" id="KW-0966">Cell projection</keyword>
    <keyword evidence="8" id="KW-0969">Cilium</keyword>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8" id="KW-0282">Flagellum</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0964">Secreted</keyword>
    <feature type="domain" description="FliD_N" evidence="6">
        <location>
            <begin position="23"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="domain" description="FliD_C" evidence="7">
        <location>
            <begin position="262"/>
            <end position="482"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009764"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023143"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU362066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF02465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF07195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ABF09146.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="498" mass="50658" checksum="29C45275431A7E86" modified="2006-05-30" version="1">MTTTSSTTSASSSGLISNLGVGSGLDLSSLLDQLTQAEQAPLTAITQEQQSYQTKLSAYGQIQSMLAAFQGSASQLASQSFFQATTASSSNTAVLSAAGSNTAATGNYAVNVTQLAQAQSLVSKGQANQAAAIGTGTIHVDFGSISGGTLDSNTSSATYGTYTGATFTANSANTGVDITIDSSHNTLQGIASAINNANAGVTASIINDGSGTPYRLVINSNNTGATNSVRLSVNETDGGTALANLVGYDPAGTQNLQQTVAAQNSQLTVNNIAIQSTSNTVSDAVAGVTMTLAQTGTSSVSVQRDTSSIMTGIQGFVTAYNNLQSTSASLSAYDPSTQTASPLTGDSTLSIMQSQMRSLLNTPQSNNALTTLSQIGISFQDDGTLAVDTSKLTTALNNNAGAVANLFGNTDGVSGYGNQISTLVKNLTSSTGALTTATAGINNTLKDLSNQYDQTQTQINADIANYKAQFTQLDVIMSQMQSTSAYLTQQFSTSSSKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="121" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LLB8</accession>
    <name>Q1LLB8_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Phosphate regulon sensor protein PhoR</fullName>
            <ecNumber evidence="2">2.7.13.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">phoR</name>
        <name evidence="10" type="ordered locus">Rmet_2179</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Member of the two-component regulatory system PhoR/PhoB involved in the phosphate regulon genes expression. PhoR may function as a membrane-associated protein kinase that phosphorylates PhoB in response to environmental signals.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.</text>
            <dbReference type="EC" id="2.7.13.3"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.7.13.3" evidence="2"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09058.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516889.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2179"/>
    <dbReference type="EnsemblBacteria" id="ABF09058">
        <property type="protein sequence ID" value="ABF09058"/>
        <property type="gene ID" value="Rmet_2179"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2179"/>
    <dbReference type="eggNOG" id="COG5002">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_89_2_4"/>
    <dbReference type="OMA" id="TRFWRGS"/>
    <dbReference type="OrthoDB" id="1827824at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004721">
        <property type="term" value="F:phosphoprotein phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006817">
        <property type="term" value="P:phosphate ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00082">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003661">
        <property type="entry name" value="HisK_dim/P"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036097">
        <property type="entry name" value="HisK_dim/P_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000014">
        <property type="entry name" value="PAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035965">
        <property type="entry name" value="PAS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021766">
        <property type="entry name" value="PhoR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004358">
        <property type="entry name" value="Sig_transdc_His_kin-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014310">
        <property type="entry name" value="Sig_transdc_His_kinase_PhoR"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00512">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13188">
        <property type="entry name" value="PAS_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11808">
        <property type="entry name" value="PhoR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00344">
        <property type="entry name" value="BCTRLSENSOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00388">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47384">
        <property type="entry name" value="SSF47384"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55785">
        <property type="entry name" value="SSF55785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="SSF55874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02966">
        <property type="entry name" value="phoR_proteo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="10" id="KW-0418">Kinase</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0592">Phosphate transport</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0808">Transferase</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="9"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="32"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="9">
        <location>
            <begin position="213"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="431"/>
            <end position="450"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00019665"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00025207"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ABF09058.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="450" mass="50710" checksum="B8C86AAE77920A49" modified="2006-05-30" version="1">MNVIWARSVAILVSLTVVATGLYVFAGPVSGLLFLCIALFLLLGYYLFQINRLWKVLDAPAYGEIPSALGLWGEVYYRLHRLVKRWRTQVLQVEQQHTRFIQAIQASPYGVLMLDDADQIEWCNDVAEQHFGLNAKRDVRQRITHLIRRPEFVHYLTRERYDEPLIMRDMGEHKRSSTSVQILPYGENRKLVLTQDITKVENTEAMRRDFVANVSHELKTPLTVLTGFLETVRDLPVSEEDRKRYIDMMLVQSMRMQHIVEDLLALAKLESDVQPPTLDPVDVGGLAAHLVHDAEALSQGRHDIHAEIDTAVVLRGSEGELLSALGNLVSNAVRYTPDGGRIAIRMTYTDGHSVFSVSDTGLGIAPEHIPRLTERFYRVDRSRSRDTGGTGLGLAIVKHVLSRHHADLRVTSEVGRGSTFRIVFPVDRSGYEPESAQPMPQLGDAPRHAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="68" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LLZ5</accession>
    <name>Q1LLZ5_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">GCN5-related N-acetyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_1952</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08831.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516679.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1952"/>
    <dbReference type="EnsemblBacteria" id="ABF08831">
        <property type="protein sequence ID" value="ABF08831"/>
        <property type="gene ID" value="Rmet_1952"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1952"/>
    <dbReference type="eggNOG" id="COG0456">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1425844_0_0_4"/>
    <dbReference type="OMA" id="ICIQRAR"/>
    <dbReference type="OrthoDB" id="1983082at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008080">
        <property type="term" value="F:N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00583">
        <property type="entry name" value="Acetyltransf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="SSF55729"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="1">
        <location>
            <begin position="12"/>
            <end position="183"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF08831.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="192" mass="21192" checksum="EAD3AFD136D685D4" modified="2006-05-30" version="1">MSTLPSQNSPRFRFRLAATYDWGDIAAVTQHAYGQYELEIMEDCRASFQRGMQSVLAAKDNPDMEWWVAETDHGISGAVLFCHPDATVPALDGSMVTLTQPEARLLSVSPQARGLGLGHRLMQICIQRARDIGSKTLVCRTMPEMDSANRLCQQMGFEKRTEAGARHGAMARLIDYVYPITAETAEATAPKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LM74</accession>
    <name>Q1LM74_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative amidase</fullName>
            <ecNumber evidence="2">6.3.5.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_1873</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EC" id="6.3.5.-" evidence="2"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08752.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516599.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1873"/>
    <dbReference type="EnsemblBacteria" id="ABF08752">
        <property type="protein sequence ID" value="ABF08752"/>
        <property type="gene ID" value="Rmet_1873"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1873"/>
    <dbReference type="eggNOG" id="COG0154">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009600_0_3_4"/>
    <dbReference type="OMA" id="TLINMID"/>
    <dbReference type="OrthoDB" id="1239251at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016874">
        <property type="term" value="F:ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1300.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000120">
        <property type="entry name" value="Amidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020556">
        <property type="entry name" value="Amidase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023631">
        <property type="entry name" value="Amidase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036928">
        <property type="entry name" value="AS_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11895">
        <property type="entry name" value="PTHR11895"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01425">
        <property type="entry name" value="Amidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75304">
        <property type="entry name" value="SSF75304"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00571">
        <property type="entry name" value="AMIDASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0436">Ligase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Amidase" evidence="1">
        <location>
            <begin position="27"/>
            <end position="433"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF08752.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="450" mass="46653" checksum="C47D96EC27534BED" modified="2006-05-30" version="1">MLPDIASLNAAVRAGTTSRADLLAEAATKATSAAGHAVYLHTTFDAAIEVARAADAADRAGKPLHPLAGLPVSVKDLYDIAGEVTRAASAARQDAAPATADATVVARLRAAGAALIGRTNMTEFAFSGVGINPHFGTPVNPCDSSVARIPGGSSSGAAVSVAIGTAVAGLGSDTGGSIRIPAALCGIVGFKPTSRRVPLTGAFPLSYTLDTACAMARTLTDCIAVDSVIADVPFIPQMKASTAIHLAIPRQLVLDDLDEPVSKAFDRAIGRLSAAGVRIEHVDMPELTELASLNAAGGFSAPESYAHHRALLAIHRDLYDPRVVTRIERGAAMSAADYIALHRARLDWIARMEARMAPFDAVVCPTVPMVAPPIAPLVEDDTLFFRTNGLLLRNTSAFNFLDGCSVSLPCHAPDELPVGLMLSQGPMRDAQLLGTALALESLVHPSRRGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LM75</accession>
    <name>Q1LM75_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Diguanylate cyclase</fullName>
            <ecNumber evidence="7">2.7.7.65</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Cellulose synthesis regulatory protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ordered locus">Rmet_1872</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>2 GTP = cyclic di-3',5'-guanylate + 2 diphosphate</text>
            <dbReference type="Rhea" id="RHEA:24898"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:58805"/>
            <dbReference type="EC" id="2.7.7.65"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="5">Glycan metabolism; bacterial cellulose biosynthesis.</text>
    </comment>
    <comment type="pathway">
        <text evidence="4">Purine metabolism; 3',5'-cyclic di-GMP biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell inner membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.7.7.65" evidence="7"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08751.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516598.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1872"/>
    <dbReference type="EnsemblBacteria" id="ABF08751">
        <property type="protein sequence ID" value="ABF08751"/>
        <property type="gene ID" value="Rmet_1872"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1872"/>
    <dbReference type="eggNOG" id="COG3706">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_464486_0_0_4"/>
    <dbReference type="OMA" id="EIWLVHE"/>
    <dbReference type="OrthoDB" id="1635706at2"/>
    <dbReference type="UniPathway" id="UPA00599"/>
    <dbReference type="UniPathway" id="UPA00694"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052621">
        <property type="term" value="F:diguanylate cyclase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030244">
        <property type="term" value="P:cellulose biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01949">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033416">
        <property type="entry name" value="CHASE7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000160">
        <property type="entry name" value="GGDEF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029787">
        <property type="entry name" value="Nucleotide_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17151">
        <property type="entry name" value="CHASE7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00990">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00267">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55073">
        <property type="entry name" value="SSF55073"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00254">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50887">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="8" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10" id="KW-0135">Cellulose biosynthesis</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0808">Transferase</keyword>
    <keyword evidence="12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="31"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="364"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="domain" description="GGDEF" evidence="13">
        <location>
            <begin position="434"/>
            <end position="572"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004429"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004665"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00005186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00012528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00031311"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ABF08751.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="586" mass="64030" checksum="4C8BE1F2A029C1EE" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LM94</accession>
    <name>Q1LM94_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Acetyl-CoA acetyltransferase / thiolase</fullName>
            <ecNumber evidence="7">2.3.1.9</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">atoB</name>
        <name evidence="7" type="ordered locus">Rmet_1853</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the thiolase-like superfamily. Thiolase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.9" evidence="7"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08732.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008650233.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1853"/>
    <dbReference type="EnsemblBacteria" id="ABF08732">
        <property type="protein sequence ID" value="ABF08732"/>
        <property type="gene ID" value="Rmet_1853"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1853"/>
    <dbReference type="eggNOG" id="COG0183">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031026_1_1_4"/>
    <dbReference type="OMA" id="SMRPDDL"/>
    <dbReference type="OrthoDB" id="330994at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003985">
        <property type="term" value="F:acetyl-CoA C-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd00751">
        <property type="entry name" value="thiolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.47.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002155">
        <property type="entry name" value="Thiolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016039">
        <property type="entry name" value="Thiolase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020615">
        <property type="entry name" value="Thiolase_acyl_enz_int_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020617">
        <property type="entry name" value="Thiolase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020613">
        <property type="entry name" value="Thiolase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020616">
        <property type="entry name" value="Thiolase_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02803">
        <property type="entry name" value="Thiolase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00108">
        <property type="entry name" value="Thiolase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000429">
        <property type="entry name" value="Ac-CoA_Ac_transf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53901">
        <property type="entry name" value="SSF53901"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01930">
        <property type="entry name" value="AcCoA-C-Actrans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00098">
        <property type="entry name" value="THIOLASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00737">
        <property type="entry name" value="THIOLASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4 7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Thiolase_N" evidence="5">
        <location>
            <begin position="5"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="domain" description="Thiolase_C" evidence="6">
        <location>
            <begin position="270"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="active site" description="Acyl-thioester intermediate" evidence="3">
        <location>
            <position position="90"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="3">
        <location>
            <position position="348"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="3">
        <location>
            <position position="378"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR000429-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF02803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABF08732.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="392" mass="40948" checksum="48EE528078EB015D" modified="2006-05-30" version="1">MNEAVIVSTARTGLAKSWKGAFNMTHGATLGGHAVQHAIARAKIEAGEVEDVLMGCANPEGATGANIARQIALRAGCPVTVPGATVNRFCSSGLQTIAMAAQRVIADEGDIFVAGGVESISCVQQDMNRHMIQESWLNKNKPEIYWSMLQTAENVAKRYNIGKDRQDEYGVQSQLRAAAALEAGKFKDEIVPMTVLAGVADKATGQLMTKEVTIDADEGIRADTTLEAVSKIRTAMPGGVITAGNASQFSDGASAAVVMNARVAAAKGLQPLGVFRGFAVAGCEPDEMGIGPVFAVPKLLKKAGLKVDDIGLWELNEAFAVQVLYCADKLGIPMDRLNVNGGAIAVGHPYGVSGARLVGHALIEGKRRGVKYVVVTMCIGGGQGAAGLFEVL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-02-26" modified="2022-08-03" version="98" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LMC7</accession>
    <name>PURT_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Formate-dependent phosphoribosylglycinamide formyltransferase</fullName>
            <ecNumber evidence="1">6.3.1.21</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">5'-phosphoribosylglycinamide transformylase 2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Formate-dependent GAR transformylase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">GAR transformylase 2</fullName>
            <shortName evidence="1">GART 2</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Non-folate glycinamide ribonucleotide transformylase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Phosphoribosylglycinamide formyltransferase 2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">purT</name>
        <name type="ordered locus">Rmet_1820</name>
    </gene>
    <organism>
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in the de novo purine biosynthesis. Catalyzes the transfer of formate to 5-phospho-ribosyl-glycinamide (GAR), producing 5-phospho-ribosyl-N-formylglycinamide (FGAR). Formate is provided by PurU via hydrolysis of 10-formyl-tetrahydrofolate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + formate + N(1)-(5-phospho-beta-D-ribosyl)glycinamide = ADP + H(+) + N(2)-formyl-N(1)-(5-phospho-beta-D-ribosyl)glycinamide + phosphate</text>
            <dbReference type="Rhea" id="RHEA:24829"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15740"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:143788"/>
            <dbReference type="ChEBI" id="CHEBI:147286"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.1.21"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="1">
            <dbReference type="Rhea" id="RHEA:24830"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Purine metabolism; IMP biosynthesis via de novo pathway; N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide from N(1)-(5-phospho-D-ribosyl)glycinamide (formate route): step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the PurK/PurT family.</text>
    </comment>
    <dbReference type="EC" id="6.3.1.21" evidence="1"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08699.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q1LMC7"/>
    <dbReference type="SMR" id="Q1LMC7"/>
    <dbReference type="STRING" id="266264.Rmet_1820"/>
    <dbReference type="EnsemblBacteria" id="ABF08699">
        <property type="protein sequence ID" value="ABF08699"/>
        <property type="gene ID" value="Rmet_1820"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1820"/>
    <dbReference type="eggNOG" id="COG0027">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
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</entry>
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                <person name="Monsieurs P."/>
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            <begin position="1008"/>
            <end position="1029"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="1036"/>
            <end position="1054"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="1060"/>
            <end position="1082"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF08555.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="1093" mass="117121" checksum="F26C2B9BA7B342D0" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-02-05" modified="2022-08-03" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LNF2</accession>
    <name>DXR_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">1-deoxy-D-xylulose 5-phosphate reductoisomerase</fullName>
            <shortName evidence="1">DXP reductoisomerase</shortName>
            <ecNumber evidence="1">1.1.1.267</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">1-deoxyxylulose-5-phosphate reductoisomerase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">2-C-methyl-D-erythritol 4-phosphate synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">dxr</name>
        <name type="ordered locus">Rmet_1441</name>
    </gene>
    <organism>
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the NADP-dependent rearrangement and reduction of 1-deoxy-D-xylulose-5-phosphate (DXP) to 2-C-methyl-D-erythritol 4-phosphate (MEP).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>2-C-methyl-D-erythritol 4-phosphate + NADP(+) = 1-deoxy-D-xylulose 5-phosphate + H(+) + NADPH</text>
            <dbReference type="Rhea" id="RHEA:13717"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:57792"/>
            <dbReference type="ChEBI" id="CHEBI:58262"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.1.1.267"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="1">Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 1/6.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the DXR family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.267" evidence="1"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08324.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516191.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q1LNF2"/>
    <dbReference type="SMR" id="Q1LNF2"/>
    <dbReference type="STRING" id="266264.Rmet_1441"/>
    <dbReference type="EnsemblBacteria" id="ABF08324">
        <property type="protein sequence ID" value="ABF08324"/>
        <property type="gene ID" value="Rmet_1441"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1441"/>
    <dbReference type="eggNOG" id="COG0743">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035714_4_0_4"/>
    <dbReference type="OMA" id="PIDSEHF"/>
    <dbReference type="OrthoDB" id="1200722at2"/>
    <dbReference type="UniPathway" id="UPA00056">
        <property type="reaction ID" value="UER00092"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030604">
        <property type="term" value="F:1-deoxy-D-xylulose-5-phosphate reductoisomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070402">
        <property type="term" value="F:NADPH binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019288">
        <property type="term" value="P:isopentenyl diphosphate biosynthetic process, methylerythritol 4-phosphate pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016114">
        <property type="term" value="P:terpenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00183">
        <property type="entry name" value="DXP_reductoisom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003821">
        <property type="entry name" value="DXP_reductoisomerase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013644">
        <property type="entry name" value="DXP_reductoisomerase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013512">
        <property type="entry name" value="DXP_reductoisomerase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026877">
        <property type="entry name" value="DXPR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036169">
        <property type="entry name" value="DXPR_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30525">
        <property type="entry name" value="PTHR30525"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08436">
        <property type="entry name" value="DXP_redisom_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02670">
        <property type="entry name" value="DXP_reductoisom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13288">
        <property type="entry name" value="DXPR_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006205">
        <property type="entry name" value="Dxp_reductismrs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69055">
        <property type="entry name" value="SSF69055"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00243">
        <property type="entry name" value="Dxr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0414">Isoprene biosynthesis</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0521">NADP</keyword>
    <keyword id="KW-0560">Oxidoreductase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_1000058417" description="1-deoxy-D-xylulose 5-phosphate reductoisomerase">
        <location>
            <begin position="1"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="7"/>
            <end position="36"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="125"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="150"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="152"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="152"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="179"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="202"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="224"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="224"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00183"/>
        </source>
    </evidence>
    <sequence length="392" mass="41053" checksum="6478EB99C236A81B" modified="2006-05-30" version="1">MQRITILGATGSIGESTLDVVRRHPDRYSVHALSAHRQVDKLAAACVEFRPARAVVGSAEAARELEAKLRAAGVPTEVSYGETALESIAEDAGTDAVMAAIVGAAGLRSSLAAARAGKRVLLANKESLVMSGGIFMDAVREHGATLLPIDSEHNAIFQCLPTSDPRYGAGVSKVLLTASGGPFRTRDPSTLHDITPDQACAHPKWVMGRKISVDSATMMNKGLEVIEAHWLFGAPAEKIEVLIHPQSIVHSMVAYADGSVLAQLGNPDMRTPIAYGMAYPERIDSGVTPLDLTVAGGLHFETPDLERFPCLGLAFDALRAGGVAPAVLNAANEVAVEAFLNGKIRFTDIARVVATVLEQPADGSADTLEGVLAADSAARRGAQAQLGSLAGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LNX4</accession>
    <name>Q1LNX4_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Outer membrane efflux family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">oprN</name>
        <name evidence="3" type="ordered locus">Rmet_1267</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the outer membrane factor (OMF) (TC 1.B.17) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08152.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516038.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1267"/>
    <dbReference type="EnsemblBacteria" id="ABF08152">
        <property type="protein sequence ID" value="ABF08152"/>
        <property type="gene ID" value="Rmet_1267"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1267"/>
    <dbReference type="eggNOG" id="COG1538">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012817_13_0_4"/>
    <dbReference type="OMA" id="FQQVQDG"/>
    <dbReference type="OrthoDB" id="1619477at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015562">
        <property type="term" value="F:efflux transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003423">
        <property type="entry name" value="OMP_efflux"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010131">
        <property type="entry name" value="RND_efflux_OM_lipoprot_NodT"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02321">
        <property type="entry name" value="OEP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01845">
        <property type="entry name" value="outer_NodT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0564">Palmitate</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1134">Transmembrane beta strand</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU362097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF08152.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="512" mass="54275" checksum="D56CCA8F137DE5F0" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LPF4</accession>
    <name>Q1LPF4_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">3-methyladenine DNA glycosylase/8-oxoguanine DNA glycosylase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_1086</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07972.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515874.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1086"/>
    <dbReference type="EnsemblBacteria" id="ABF07972">
        <property type="protein sequence ID" value="ABF07972"/>
        <property type="gene ID" value="Rmet_1086"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1086"/>
    <dbReference type="eggNOG" id="COG0122">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_72_5_4"/>
    <dbReference type="OMA" id="WYIWRSL"/>
    <dbReference type="OrthoDB" id="1781735at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006284">
        <property type="term" value="P:base-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00056">
        <property type="entry name" value="ENDO3c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011257">
        <property type="entry name" value="DNA_glycosylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003265">
        <property type="entry name" value="HhH-GPD_domain"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00730">
        <property type="entry name" value="HhH-GPD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00478">
        <property type="entry name" value="ENDO3c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48150">
        <property type="entry name" value="SSF48150"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ENDO3c" evidence="2">
        <location>
            <begin position="103"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="42"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF07972.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="261" mass="28828" checksum="07820A1ECBA4FDA1" modified="2006-05-30" version="1">MNAAARKPKAVAPAQPKPVAGKAAAKVAPKAGGKPRKSDLPEVDAVPLPVETVVDAVRPAYWDEACADLMKRDRILRKMIPTYGPAHLVSRGDPFVTLARSIVGQQISVKAAQSVWERLAARCPRLTPAQFLKSGSEELAGCGLSKRKAEYIIDLADHFKAGRVHVKEWVAMDDEAVIAELTQIRGIGRWTAEMFLMFNLMRPNVLPLDDVGLINAISTNYFSGEPVTRSEAREVAANWEPWRTVATWYMWRSLDPLPVTY</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="117" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LQ63</accession>
    <name>Q1LQ63_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Putative cation/proton antiporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">cvrA</name>
        <name evidence="8" type="ordered locus">Rmet_0827</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07713.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515656.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0827"/>
    <dbReference type="EnsemblBacteria" id="ABF07713">
        <property type="protein sequence ID" value="ABF07713"/>
        <property type="gene ID" value="Rmet_0827"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0827"/>
    <dbReference type="eggNOG" id="COG3263">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005912_9_2_4"/>
    <dbReference type="OMA" id="WFDEYGE"/>
    <dbReference type="OrthoDB" id="1194802at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005451">
        <property type="term" value="F:monovalent cation:proton antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006813">
        <property type="term" value="P:potassium ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.465.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006153">
        <property type="entry name" value="Cation/H_exchanger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036318">
        <property type="entry name" value="FAD-bd_PCMH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016169">
        <property type="entry name" value="FAD-bd_PCMH_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030151">
        <property type="entry name" value="NhaP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006037">
        <property type="entry name" value="RCK_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005170">
        <property type="entry name" value="Transptr-assoc_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32507:SF7">
        <property type="entry name" value="PTHR32507:SF7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03471">
        <property type="entry name" value="CorC_HlyC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00999">
        <property type="entry name" value="Na_H_Exchanger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01091">
        <property type="entry name" value="CorC_HlyC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56176">
        <property type="entry name" value="SSF56176"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51202">
        <property type="entry name" value="RCK_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0406">Ion transport</keyword>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0630">Potassium</keyword>
    <keyword evidence="1" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="6"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="32"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="57"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="90"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="119"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="191"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="224"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="273"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="298"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="334"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="366"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="domain" description="RCK C-terminal" evidence="7">
        <location>
            <begin position="403"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="576"/>
            <end position="605"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ABF07713.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="605" mass="64141" checksum="1F5C49F087A6E6BE" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LQC6</accession>
    <name>Q1LQC6_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Carbon-phosphorus lyase complex subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">phnI</name>
        <name evidence="2" type="ordered locus">Rmet_0764</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07650.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515605.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0764"/>
    <dbReference type="EnsemblBacteria" id="ABF07650">
        <property type="protein sequence ID" value="ABF07650"/>
        <property type="gene ID" value="Rmet_0764"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0764"/>
    <dbReference type="eggNOG" id="COG3626">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_063686_0_0_4"/>
    <dbReference type="OMA" id="AQFTRGY"/>
    <dbReference type="OrthoDB" id="1022121at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019634">
        <property type="term" value="P:organic phosphonate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008773">
        <property type="entry name" value="PhnI"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05861">
        <property type="entry name" value="PhnI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF007313">
        <property type="entry name" value="PhnI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0456">Lyase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="354"/>
            <end position="373"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF07650.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="373" mass="41037" checksum="DEFD20F5F0C2D9FB" modified="2006-05-30" version="1">MYVAVKGGERAILNSYQMLDAYRRGDTSVPEITLEQIREQMPLAVSRVMAEGSLYDPHLAALALKQAAGDQIEAIFLLRAYRTTLARFGYTQALDTGKMCLQRRISSTFKDVPGGQVLGPTYDYTQRLLDFTLEAGRDPAPLPPSPEPLAPNMPRVTSLLEAESLVEADAIPPGDPAPPDLTREPLTFPAERPARLQNLARADEGFLLSMGYSTQRGYGNSHPFAGEIRYGSTEVEMFIEELGFAITIGEVEVTECQMVSQFAGNADEAPRLTRGYGLVFGYNERKAMSMALADRAMRAEELGEAGDAPANNIEFMLYHSDNVEASGFVQHLKLPHYVDFQANLELLRRLRAEQQGAATDAPAEDQPQEESLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-10-07" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LQE5</accession>
    <name>Q1LQE5_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_0745</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07631.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0745"/>
    <dbReference type="EnsemblBacteria" id="ABF07631">
        <property type="protein sequence ID" value="ABF07631"/>
        <property type="gene ID" value="Rmet_0745"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0745"/>
    <dbReference type="HOGENOM" id="CLU_2411055_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF07631.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="92" mass="10139" checksum="21C624D4D84BE4BB" modified="2006-05-30" version="1">MNSTDNDKWPACRARSEKSGDMMNLDKDVLWRLRPTLVSISGYRAEAGQVGCRLRNDGTLCDSGQRRAAACAALISTHKCRDKGERVEVPAT</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-10-31" modified="2022-08-03" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LQG2</accession>
    <name>HLDD_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">ADP-L-glycero-D-manno-heptose-6-epimerase</fullName>
            <ecNumber evidence="1">5.1.3.20</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">ADP-L-glycero-beta-D-manno-heptose-6-epimerase</fullName>
            <shortName evidence="1">ADP-glyceromanno-heptose 6-epimerase</shortName>
            <shortName evidence="1">ADP-hep 6-epimerase</shortName>
            <shortName evidence="1">AGME</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">hldD</name>
        <name type="ordered locus">Rmet_0728</name>
    </gene>
    <organism>
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the interconversion between ADP-D-glycero-beta-D-manno-heptose and ADP-L-glycero-beta-D-manno-heptose via an epimerization at carbon 6 of the heptose.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ADP-D-glycero-beta-D-manno-heptose = ADP-L-glycero-beta-D-manno-heptose</text>
            <dbReference type="Rhea" id="RHEA:17577"/>
            <dbReference type="ChEBI" id="CHEBI:59967"/>
            <dbReference type="ChEBI" id="CHEBI:61506"/>
            <dbReference type="EC" id="5.1.3.20"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </cofactor>
        <text evidence="1">Binds 1 NADP(+) per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Nucleotide-sugar biosynthesis; ADP-L-glycero-beta-D-manno-heptose biosynthesis; ADP-L-glycero-beta-D-manno-heptose from D-glycero-beta-D-manno-heptose 7-phosphate: step 4/4.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homopentamer.</text>
    </comment>
    <comment type="domain">
        <text evidence="1">Contains a large N-terminal NADP-binding domain, and a smaller C-terminal substrate-binding domain.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the NAD(P)-dependent epimerase/dehydratase family. HldD subfamily.</text>
    </comment>
    <dbReference type="EC" id="5.1.3.20" evidence="1"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07614.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515575.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q1LQG2"/>
    <dbReference type="SMR" id="Q1LQG2"/>
    <dbReference type="STRING" id="266264.Rmet_0728"/>
    <dbReference type="EnsemblBacteria" id="ABF07614">
        <property type="protein sequence ID" value="ABF07614"/>
        <property type="gene ID" value="Rmet_0728"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0728"/>
    <dbReference type="eggNOG" id="COG0451">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007383_1_3_4"/>
    <dbReference type="OMA" id="LRDFVYI"/>
    <dbReference type="OrthoDB" id="1180629at2"/>
    <dbReference type="UniPathway" id="UPA00356">
        <property type="reaction ID" value="UER00440"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008712">
        <property type="term" value="F:ADP-glyceromanno-heptose 6-epimerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097171">
        <property type="term" value="P:ADP-L-glycero-beta-D-manno-heptose biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd05248">
        <property type="entry name" value="ADP_GME_SDR_e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01601">
        <property type="entry name" value="Heptose_epimerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001509">
        <property type="entry name" value="Epimerase_deHydtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011912">
        <property type="entry name" value="Heptose_epim"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01370">
        <property type="entry name" value="Epimerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02197">
        <property type="entry name" value="heptose_epim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword id="KW-0413">Isomerase</keyword>
    <keyword id="KW-0521">NADP</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000255738" description="ADP-L-glycero-D-manno-heptose-6-epimerase">
        <location>
            <begin position="1"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="1">
        <location>
            <position position="139"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="1">
        <location>
            <position position="177"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="11"/>
            <end position="12"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="32"/>
            <end position="33"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="39"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="54"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="75"/>
            <end position="79"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="92"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="143"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="168"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="169"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="177"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="179"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="186"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="200"/>
            <end position="203"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="213"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="292"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01601"/>
        </source>
    </evidence>
    <sequence length="331" mass="37354" checksum="7D72B08B70E48514" modified="2006-05-30" version="1">MTIIVTGAAGFIGSNIVKGLNERGETNIIAVDNLTRAEKFNNLVDCEIADYLDKTDFVARFARGDFGNVRAVFHEGACSDTMETDGRYMMENNYRYTLALLEACLEQGAQFLYASSAATYGASTMFREDRDYEKPLNVYGYSKFLFDQVVRRRLPSAHSQIVGFRYFNVYGPREFHKGRMASVAFHHFNQFRAEGTVKLFGEYNGYAPGTQSRDFVSVEDVVKVNLYFLDHPEKSGIFNLGTGRSQPFNDIAVSVVNALRESEGKPALSLDEMVQEGLVEYVKFPDALRGKYQCFTQSDVSRLRGAGYSDQFLTVQEGVSRYCKWLLERSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="65" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LQI8</accession>
    <name>Q1LQI8_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_0702</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the AHA1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07588.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515552.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0702"/>
    <dbReference type="EnsemblBacteria" id="ABF07588">
        <property type="protein sequence ID" value="ABF07588"/>
        <property type="gene ID" value="Rmet_0702"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0702"/>
    <dbReference type="eggNOG" id="COG3832">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_108923_4_0_4"/>
    <dbReference type="OMA" id="FSRQYFF"/>
    <dbReference type="OrthoDB" id="1498264at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.530.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013538">
        <property type="entry name" value="Activator_of_Hsp90_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023393">
        <property type="entry name" value="START-like_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08327">
        <property type="entry name" value="AHSA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006817"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF07588.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="158" mass="17886" checksum="A8FED6B7854A5ACE" modified="2006-05-30" version="1">MAIPQTIVVTYIAATPEQVWGALTDPTQSTQYFFGRRVESDWKVDSPFVLRMEDGQVDSQGVVLTADAPYRLSVTWRVEWLEAFRQLPEVIVTWQIDALGDEAVRLTVSETHREPVDEQILQGGRNGWPMIVAGLKTWLETGRRLKLETPQPPPAPDQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="103" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LQS2</accession>
    <name>Q1LQS2_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Chaperone clpB (Heat-shock protein F84.1)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">clpB</name>
        <name evidence="12" type="ordered locus">Rmet_0618</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Part of a stress-induced multi-chaperone system, it is involved in the recovery of the cell from heat-induced damage, in cooperation with DnaK, DnaJ and GrpE. Acts before DnaK, in the processing of protein aggregates. Protein binding stimulates the ATPase activity; ATP hydrolysis unfolds the denatured protein aggregates, which probably helps expose new hydrophobic binding sites on the surface of ClpB-bound aggregates, contributing to the solubilization and refolding of denatured protein aggregates by DnaK.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ClpA/ClpB family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07504.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515475.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0618"/>
    <dbReference type="EnsemblBacteria" id="ABF07504">
        <property type="protein sequence ID" value="ABF07504"/>
        <property type="gene ID" value="Rmet_0618"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0618"/>
    <dbReference type="eggNOG" id="COG0542">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005070_1_0_4"/>
    <dbReference type="OMA" id="CEDFTAK"/>
    <dbReference type="OrthoDB" id="44062at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1780.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017729">
        <property type="entry name" value="ATPase_T6SS_ClpV1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019489">
        <property type="entry name" value="Clp_ATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036628">
        <property type="entry name" value="Clp_N_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004176">
        <property type="entry name" value="Clp_R_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001270">
        <property type="entry name" value="ClpA/B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018368">
        <property type="entry name" value="ClpA/B_CS1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041546">
        <property type="entry name" value="ClpA/ClpB_AAA_lid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="AAA_lid_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02861">
        <property type="entry name" value="Clp_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10431">
        <property type="entry name" value="ClpB_D2-small"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00300">
        <property type="entry name" value="CLPPROTEASEA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01086">
        <property type="entry name" value="ClpB_D2-small"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81923">
        <property type="entry name" value="SSF81923"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03345">
        <property type="entry name" value="VI_ClpV1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51903">
        <property type="entry name" value="CLP_R"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00870">
        <property type="entry name" value="CLPAB_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0143">Chaperone</keyword>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Clp R" evidence="11">
        <location>
            <begin position="10"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="164"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="472"/>
            <end position="499"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008675"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00025613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51903"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ABF07504.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="893" mass="97474" checksum="F2A5A0115A3534A5" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="99" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LQS6</accession>
    <name>Q1LQS6_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Autotransporter domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Rmet_0614</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07500.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515473.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0614"/>
    <dbReference type="EnsemblBacteria" id="ABF07500">
        <property type="protein sequence ID" value="ABF07500"/>
        <property type="gene ID" value="Rmet_0614"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0614"/>
    <dbReference type="eggNOG" id="COG1652">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG2982">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG3468">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG4625">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_239670_0_0_4"/>
    <dbReference type="OMA" id="YPEVHAF"/>
    <dbReference type="OrthoDB" id="1713183at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019867">
        <property type="term" value="C:outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01344">
        <property type="entry name" value="PL2_Passenger_AT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.20.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.20.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.128.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043990">
        <property type="entry name" value="AC_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005546">
        <property type="entry name" value="Autotransporte_beta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036709">
        <property type="entry name" value="Autotransporte_beta_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012332">
        <property type="entry name" value="Autotransporter_pectin_lyase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013425">
        <property type="entry name" value="Autotrns_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043709">
        <property type="entry name" value="DUF5649"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024973">
        <property type="entry name" value="ESPR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008638">
        <property type="entry name" value="Filamn_hemagglutn_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006315">
        <property type="entry name" value="OM_autotransptr_brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012334">
        <property type="entry name" value="Pectin_lyas_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011050">
        <property type="entry name" value="Pectin_lyase_fold/virulence"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18883">
        <property type="entry name" value="AC_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03797">
        <property type="entry name" value="Autotransporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18886">
        <property type="entry name" value="DUF5649"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13018">
        <property type="entry name" value="ESPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12951">
        <property type="entry name" value="PATR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00869">
        <property type="entry name" value="Autotransporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00912">
        <property type="entry name" value="Haemagg_act"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103515">
        <property type="entry name" value="SSF103515"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51126">
        <property type="entry name" value="SSF51126"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01414">
        <property type="entry name" value="autotrans_barl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02601">
        <property type="entry name" value="autotrns_rpt"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51208">
        <property type="entry name" value="AUTOTRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <feature type="domain" description="Autotransporter" evidence="4">
        <location>
            <begin position="1462"/>
            <end position="1741"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1378"/>
            <end position="1407"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1439"/>
            <end position="1467"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1379"/>
            <end position="1406"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1446"/>
            <end position="1460"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51208"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABF07500.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="1741" mass="169868" checksum="18A50D6DACC47D4A" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="103" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LQS8</accession>
    <name>Q1LQS8_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Two component transcriptional regulator, LuxR family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Rmet_0612</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07498.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0612"/>
    <dbReference type="EnsemblBacteria" id="ABF07498">
        <property type="protein sequence ID" value="ABF07498"/>
        <property type="gene ID" value="Rmet_0612"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0612"/>
    <dbReference type="eggNOG" id="COG2197">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_90_10_4"/>
    <dbReference type="OMA" id="IVADDYP"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06170">
        <property type="entry name" value="LuxR_C_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016032">
        <property type="entry name" value="Sig_transdc_resp-reg_C-effctor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000792">
        <property type="entry name" value="Tscrpt_reg_LuxR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00196">
        <property type="entry name" value="GerE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00038">
        <property type="entry name" value="HTHLUXR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00421">
        <property type="entry name" value="HTH_LUXR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46894">
        <property type="entry name" value="SSF46894"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00622">
        <property type="entry name" value="HTH_LUXR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50043">
        <property type="entry name" value="HTH_LUXR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Response regulatory" evidence="4">
        <location>
            <begin position="12"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="domain" description="HTH luxR-type" evidence="3">
        <location>
            <begin position="163"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="2">
        <location>
            <position position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABF07498.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="231" mass="24602" checksum="E4E7FA4AB413E86A" modified="2006-05-30" version="1">MPDTAESVLPAPLLVVEDEPPMQSRLRTILAALGYGDDAIWFAGSLEAANTLLATQPFAMALVDVGLPDGNGIDLIRVMHEQDPALPILVISTWNTERVIVSALQAGATGYLLKERDDVEIILSIRSALRGGAPIDPFVARRILDLAGKMAPLAGGAEVQAGPTVGAPPLSAREIEILSLVSKGLTNREIAEVLSLSKLTVECHIRNSYKKLAVHSRTQAVFEARASGLLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="100" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LR07</accession>
    <name>Q1LR07_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">3-deoxy-manno-octulosonate cytidylyltransferase</fullName>
            <ecNumber evidence="3">2.7.7.38</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">CMP-2-keto-3-deoxyoctulosonic acid synthase</fullName>
            <shortName evidence="3">CKS</shortName>
            <shortName evidence="3">CMP-KDO synthase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="3 4" type="primary">kdsB</name>
        <name evidence="4" type="ordered locus">Rmet_0533</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Activates KDO (a required 8-carbon sugar) for incorporation into bacterial lipopolysaccharide in Gram-negative bacteria.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>3-deoxy-alpha-D-manno-oct-2-ulosonate + CTP = CMP-3-deoxy-beta-D-manno-octulosonate + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:23448"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
            <dbReference type="ChEBI" id="CHEBI:85986"/>
            <dbReference type="ChEBI" id="CHEBI:85987"/>
            <dbReference type="EC" id="2.7.7.38"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Bacterial outer membrane biogenesis; lipopolysaccharide biosynthesis.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3">Nucleotide-sugar biosynthesis; CMP-3-deoxy-D-manno-octulosonate biosynthesis; CMP-3-deoxy-D-manno-octulosonate from 3-deoxy-D-manno-octulosonate and CTP: step 1/1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the KdsB family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.38" evidence="3"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07419.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515395.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0533"/>
    <dbReference type="EnsemblBacteria" id="ABF07419">
        <property type="protein sequence ID" value="ABF07419"/>
        <property type="gene ID" value="Rmet_0533"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0533"/>
    <dbReference type="eggNOG" id="COG1212">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_065038_1_0_4"/>
    <dbReference type="OMA" id="FMATCAK"/>
    <dbReference type="OrthoDB" id="1345588at2"/>
    <dbReference type="UniPathway" id="UPA00030"/>
    <dbReference type="UniPathway" id="UPA00358">
        <property type="reaction ID" value="UER00476"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008690">
        <property type="term" value="F:3-deoxy-manno-octulosonate cytidylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033468">
        <property type="term" value="P:CMP-keto-3-deoxy-D-manno-octulosonic acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009103">
        <property type="term" value="P:lipopolysaccharide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02517">
        <property type="entry name" value="CMP-KDO-Synthetase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00057">
        <property type="entry name" value="KdsB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003329">
        <property type="entry name" value="Cytidylyl_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004528">
        <property type="entry name" value="KdsB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02348">
        <property type="entry name" value="CTP_transf_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="SSF53448"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00466">
        <property type="entry name" value="kdsB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 3" id="KW-0448">Lipopolysaccharide biosynthesis</keyword>
    <keyword evidence="3 4" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 3 4" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022985"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF07419.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="269" mass="28527" checksum="D761E43796FC7279" modified="2006-05-30" version="1">MSIPTFTVVIPARLASTRLPNKPLADIGGHPMIVRVAERAHASSAQRTVVATDSQEVAQACAAHGVEAVLTRADHQSGTDRLSEVAAQLGLADDAIVVNVQGDEPLIEPTLIDEVALHLAAHTDCAIATAAHPLDDVAEVFNPNVVKVVCDNAGRALYFSRAPIPWARDAWSAVPAQPAATAQVPLPAMPVLRHIGIYAYRAGFLRRFPTLAVAPVEQTEALEQLRAMWHGERIGVMVTAAAPAPGVDTPADLERVRALWVQGMAQEGP</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="63" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LR43</accession>
    <name>Q1LR43_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Branched-chain amino acid transport protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">Rmet_0497</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the AzlC family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07383.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515366.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0497"/>
    <dbReference type="EnsemblBacteria" id="ABF07383">
        <property type="protein sequence ID" value="ABF07383"/>
        <property type="gene ID" value="Rmet_0497"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0497"/>
    <dbReference type="eggNOG" id="COG1296">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_065777_1_0_4"/>
    <dbReference type="OMA" id="WLSWQVS"/>
    <dbReference type="OrthoDB" id="1866750at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011606">
        <property type="entry name" value="Brnchd-chn_aa_trnsp_permease"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34979">
        <property type="entry name" value="PTHR34979"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03591">
        <property type="entry name" value="AzlC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="86"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="119"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="150"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="180"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="220"/>
            <end position="240"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010735"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ABF07383.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="266" mass="29044" checksum="16140B2B771E6470" modified="2006-05-30" version="1">MTPRPLSPVWQRLWHRFAEVERDAFLEGARFFARFQPAIFSWGLVTGVAMSKSALTVPEAIGMSLLVYAGSAQLAVLPLFAAGMPIWTVLLTSAVVNLRFVIFSAALQPHFSYLPLWRRTVLGFFNGDLHFVYFMQRYTEPGYAPGKEGYFWGMALFNCVTWQVSSIIGILLASLFPDSWGLALAGMMALIPVMVSTINSRSTLLAVAVSSVLALLCFDLPYRLGLVVAVIGAIAAGMASDEMAARATLRGIRARKSAQPQAGDQT</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="70" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LR48</accession>
    <name>Q1LR48_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">zf-CHCC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_0492</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07378.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008652632.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0492"/>
    <dbReference type="EnsemblBacteria" id="ABF07378">
        <property type="protein sequence ID" value="ABF07378"/>
        <property type="gene ID" value="Rmet_0492"/>
    </dbReference>
    <dbReference type="GeneID" id="60824089"/>
    <dbReference type="KEGG" id="rme:Rmet_0492"/>
    <dbReference type="eggNOG" id="COG4391">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_083053_4_0_4"/>
    <dbReference type="OMA" id="DLPLYCP"/>
    <dbReference type="OrthoDB" id="1858380at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019401">
        <property type="entry name" value="Znf_CHCC"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10276">
        <property type="entry name" value="zf-CHCC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="zf-CHCC" evidence="1">
        <location>
            <begin position="19"/>
            <end position="52"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF10276"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF07378.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="64" mass="7025" checksum="5A06092DD1FEC43A" modified="2006-05-30" version="1">MTQTASVVEIGAEDMPLHCPTANTPAWNYHPRVFLDVADTGEVRCPYCGTVYKLKPGTVLKGHH</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="70" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LRC8</accession>
    <name>Q1LRC8_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative DNA binding transmembrane protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_0412</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07298.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515288.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0412"/>
    <dbReference type="EnsemblBacteria" id="ABF07298">
        <property type="protein sequence ID" value="ABF07298"/>
        <property type="gene ID" value="Rmet_0412"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0412"/>
    <dbReference type="eggNOG" id="ENOG502Z8KI">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_096797_0_0_4"/>
    <dbReference type="OMA" id="LWAFEEG"/>
    <dbReference type="OrthoDB" id="970661at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="69"/>
            <end position="124"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF07298.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="251" mass="27653" checksum="FA270C7B631DE939" modified="2006-05-30" version="1">MGVTYRPLRPRRGLRSHAAVIRQQRPWRVWVLVVALLLVGGALAAWMYEMGRRAGKPAVESFTDGSEKIAQLQQQLVDVTGERDRLRATANTAGAELQMARAAQERLAQQLKTAEADVGQLKEDLGFFESLLPATGDTSGIHVRSFRVALDEAQPRAVHYRLLAMQGGSKAFVEQPDFMGELQFTISAVRDGKPLTLTLPQPGDPPLPKVRLTHYQRIEGDLMVPQGAEVRAVMVRIVQNGQVRATQSATP</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LRD5</accession>
    <name>Q1LRD5_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Hydroxypyruvate reductase</fullName>
            <ecNumber evidence="3">1.1.1.81</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">ttuD</name>
        <name evidence="3" type="ordered locus">Rmet_0405</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EC" id="1.1.1.81" evidence="3"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07291.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515281.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0405"/>
    <dbReference type="EnsemblBacteria" id="ABF07291">
        <property type="protein sequence ID" value="ABF07291"/>
        <property type="gene ID" value="Rmet_0405"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0405"/>
    <dbReference type="eggNOG" id="COG2379">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032279_1_1_4"/>
    <dbReference type="OMA" id="RCGAPIT"/>
    <dbReference type="OrthoDB" id="1358712at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008887">
        <property type="term" value="F:glycerate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016618">
        <property type="term" value="F:hydroxypyruvate reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1480.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10180">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037035">
        <property type="entry name" value="GK-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038614">
        <property type="entry name" value="GK_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007835">
        <property type="entry name" value="MOFRL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025286">
        <property type="entry name" value="MOFRL_assoc_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039760">
        <property type="entry name" value="MOFRL_protein"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12227">
        <property type="entry name" value="PTHR12227"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13660">
        <property type="entry name" value="DUF4147"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05161">
        <property type="entry name" value="MOFRL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF4147" evidence="2">
        <location>
            <begin position="11"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="domain" description="MOFRL" evidence="1">
        <location>
            <begin position="316"/>
            <end position="425"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF05161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF07291.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="432" mass="44592" checksum="2F64E0A238975B8E" modified="2006-05-30" version="1">MNPQVDPRTLLLESFRAAVAAADPLEIVAAHLPPPHAGGRTLVVGAGKAAASMALAVERAYQGKAKLEGIVVTRYAHGLPTEHIRVIEAGHPVPDEAGEQAAADILARVQALGPDDRLIVLVSGGGSSLLSLPAEGIPMADLKATTRELLRCGAPITDMNIVRKHTSRIQGGRLAQASRAPVTTLIVSDVAGDDPSAIASGPTVPDASTYADALAILKRYGAAVPATVQAHLERGARGEVEETPKPGDPLFMNVDNRMIATAHDSLEAAAAEFRRQGVNAVVLGDTVTGEAQEVARVYAALVREIRAYNAPFAAPVALISGGECTVTLPSGASESARGGRCSEFLLSLAIELAGTPNVHAIAADTDGIDGSEDNAGALLAPDSLARAEGSGTSARQKLDAHDAWGFFDAIGDLVVTGPTRTNVNDYRAILIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="66" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LRF2</accession>
    <name>Q1LRF2_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_0388</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07274.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008645964.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0388"/>
    <dbReference type="EnsemblBacteria" id="ABF07274">
        <property type="protein sequence ID" value="ABF07274"/>
        <property type="gene ID" value="Rmet_0388"/>
    </dbReference>
    <dbReference type="GeneID" id="60823990"/>
    <dbReference type="KEGG" id="rme:Rmet_0388"/>
    <dbReference type="eggNOG" id="COG1307">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069606_0_0_4"/>
    <dbReference type="OMA" id="HYAESIP"/>
    <dbReference type="OrthoDB" id="1288126at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1180.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003797">
        <property type="entry name" value="DegV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043168">
        <property type="entry name" value="DegV_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02645">
        <property type="entry name" value="DegV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51482">
        <property type="entry name" value="DEGV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF07274.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="318" mass="34508" checksum="776D107696C5BB0B" modified="2006-05-30" version="1">MQETAILADAACDMPRAMMDALGVRTIPFRIRAGDRFVVDTRDEAELPNLYQKYLVGKQDHYAESIPLLERELEDHLLKNVVAGCDRAILLTIASSRSKLYSNATGAVVGTVARSFKLRKDAGRSGLFDLTVIDTGAIGPGQALIVREAARMLADGASAASVVDAVENRLRDAAHMFLIPDELLYMYTRAKLKGENSITWSRYMLGSAFNIRPVIHMHRGVTQAIAKARGNEEGVRRLLSHVEACIRADSLLTPAVAACYAGDIDEVESLPAWQSLKHIAASRNVELMLAPMSLTVALNVGAGAFGLSYLSEAPPPFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LRG5</accession>
    <name>Q1LRG5_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Putative Na+/solute symporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Rmet_0375</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the sodium:solute symporter (SSF) (TC 2.A.21) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07261.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515253.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0375"/>
    <dbReference type="EnsemblBacteria" id="ABF07261">
        <property type="protein sequence ID" value="ABF07261"/>
        <property type="gene ID" value="Rmet_0375"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0375"/>
    <dbReference type="eggNOG" id="COG0591">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018808_15_3_4"/>
    <dbReference type="OMA" id="GWWGMRR"/>
    <dbReference type="OrthoDB" id="231452at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1730.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038377">
        <property type="entry name" value="Na/Glc_symporter_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001734">
        <property type="entry name" value="Na/solute_symporter"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00474">
        <property type="entry name" value="SSF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50283">
        <property type="entry name" value="NA_SOLUT_SYMP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="6"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="37"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="76"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="115"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="147"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="174"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="223"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="262"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="314"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="360"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="392"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="418"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="444"/>
            <end position="463"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU362091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ABF07261.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="478" mass="51924" checksum="D4297F0325394076" modified="2006-05-30" version="1">MLIWFVIIYWIISVGIGLWAALRVKGTADFAVAGRSLPFYIVTATVFATWFGSETVLGIPAVFLKEGLSGVVSDPFGSSLCLILVGLFFARPLYRMNLLTIGDYYHNRYGRLAEVLTTLCIVVSYLGWVAAQIKALGLVFYTVSDGALSQDAGMMIGAASVLVYTLFGGMWSVAVTDFIQMIIIVIGMLYIGYEVSGQAGGVTAVVSHAAAAGKFEFLPSLDLVQIIGFAAALFTMMLGSIPQQDVFQRVTSSRTEQIAGRASVLGGVLYFFFAFIPMFLAYSATLIDPAMVEKYINSDSQLILPQLILTHAPMFAQVMFFGALLSAIKSCASATLLAPSVTFAENILRPYFRHLADKQFLRVMQVVVLVFTSLVTLFALNSHLSIFHMVENAYKVTLVSSFVPLAFGMFWKPATRQGGLAAILLGLASWLTCEIAFADATVPPQMVGLLFSISGMVFGSLLPQWISDRPPVREVHIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LRH7</accession>
    <name>Q1LRH7_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative carbon-monoxide dehydrogenase (Acceptor)</fullName>
            <ecNumber evidence="3">1.2.99.2</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">coxM</name>
        <name evidence="3" type="ordered locus">Rmet_0363</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EC" id="1.2.99.2" evidence="3"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07249.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515241.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0363"/>
    <dbReference type="EnsemblBacteria" id="ABF07249">
        <property type="protein sequence ID" value="ABF07249"/>
        <property type="gene ID" value="Rmet_0363"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0363"/>
    <dbReference type="eggNOG" id="COG1319">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_058050_3_0_4"/>
    <dbReference type="OMA" id="YEFKYHR"/>
    <dbReference type="OrthoDB" id="1017413at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.43.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.465.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005107">
        <property type="entry name" value="CO_DH_flav_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036683">
        <property type="entry name" value="CO_DH_flav_C_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016166">
        <property type="entry name" value="FAD-bd_PCMH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036318">
        <property type="entry name" value="FAD-bd_PCMH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016167">
        <property type="entry name" value="FAD-bd_PCMH_sub1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016169">
        <property type="entry name" value="FAD-bd_PCMH_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002346">
        <property type="entry name" value="Mopterin_DH_FAD-bd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03450">
        <property type="entry name" value="CO_deh_flav_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00941">
        <property type="entry name" value="FAD_binding_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01092">
        <property type="entry name" value="CO_deh_flav_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55447">
        <property type="entry name" value="SSF55447"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56176">
        <property type="entry name" value="SSF56176"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51387">
        <property type="entry name" value="FAD_PCMH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0274">FAD</keyword>
    <keyword evidence="1" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD-binding PCMH-type" evidence="2">
        <location>
            <begin position="1"/>
            <end position="170"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF07249.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="266" mass="27655" checksum="D38A4A3CA3178D86" modified="2006-05-30" version="1">MYAFQYERAADANAAVAKLKADGDAKFLAGGQSLLAAMKLRLASPSTLVDVSRIPGMNGIRVEGDALVIGAATRHADVAANADVMRRIPALAALANGIGDRQVRAMGTIGGSLANDDPAADYPAAVLGLNATVVTDRRSIAADDFFKGLYETALEPDELITAVRFPSPDQAAYIKFRNPASRFALVGVMVARTGKTVRVAVTGAADSVFRAPALEQALAASFTPAAARAVKMDPSGLNVDLHASAEYRAHLIPVLAARAVEQALKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="65" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LRN4</accession>
    <name>Q1LRN4_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Rmet_0306</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07192.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515192.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0306"/>
    <dbReference type="EnsemblBacteria" id="ABF07192">
        <property type="protein sequence ID" value="ABF07192"/>
        <property type="gene ID" value="Rmet_0306"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0306"/>
    <dbReference type="eggNOG" id="COG3117">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_100563_2_1_4"/>
    <dbReference type="OMA" id="VAGSTFW"/>
    <dbReference type="OrthoDB" id="1983716at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005887">
        <property type="term" value="C:integral component of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015221">
        <property type="term" value="F:lipopolysaccharide transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010664">
        <property type="entry name" value="LipoPS_assembly_LptC-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026265">
        <property type="entry name" value="LptC"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06835">
        <property type="entry name" value="LptC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR04409">
        <property type="entry name" value="LptC_YrbK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="1" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABF07192.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="208" mass="23062" checksum="1B51FC487978C6C1" modified="2006-05-30" version="1">MPEFLSRITGLIMRLLPLLLMAIVAGSTFWLVQINSPHEATAQAQVKRHVPDYYMDRFSATELAEDGNTKIRFTGVKMVHFEDDQTYEVTTPAMRNYEPDRPPVTANANRGVMNAEGSIIDLYGNAFIVRQAGTDVSKDPRMTAASQYFQLLVNDDIVKTDKPVELTRGPSVMNANGLIFNNVTREVQLLGNVRGTIIMNSPGQASAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="52" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LSD1</accession>
    <name>Q1LSD1_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_0059</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF06945.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011514984.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0059"/>
    <dbReference type="EnsemblBacteria" id="ABF06945">
        <property type="protein sequence ID" value="ABF06945"/>
        <property type="gene ID" value="Rmet_0059"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0059"/>
    <dbReference type="eggNOG" id="ENOG502ZT5X">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_159816_1_0_4"/>
    <dbReference type="OMA" id="DYWLQPG"/>
    <dbReference type="OrthoDB" id="1717272at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021317">
        <property type="entry name" value="DUF2917"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11142">
        <property type="entry name" value="DUF2917"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF06945.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="122" mass="13491" checksum="78941D4FB8AB0E0E" modified="2006-05-30" version="1">MKALLTTTVFTLAPGEVTALSLHAAQRLFVEDAKGMDVWVTRENDSEDYWLRCGSSLMLRRGDEIVLSVDPRAAGTVRLALIAEARRPALTLADVPHIAYRAVRRLVRGTEWTPAEDKITAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-04-26" modified="2021-09-29" version="99" xmlns="http://uniprot.org/uniprot">
    <accession>Q58AK3</accession>
    <name>Q58AK3_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transposase ISRme10</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">tnpA</name>
        <name evidence="2" type="ordered locus">Rmet_5954</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2 3">
        <name>pMOL30</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid pMOL30</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000354">
        <property type="protein sequence ID" value="ABF12813.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011229360.1">
        <property type="nucleotide sequence ID" value="NC_007971.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_145615.1">
        <property type="nucleotide sequence ID" value="NC_006466.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF12813">
        <property type="protein sequence ID" value="ABF12813"/>
        <property type="gene ID" value="Rmet_5954"/>
    </dbReference>
    <dbReference type="GeneID" id="60823105"/>
    <dbReference type="KEGG" id="rme:Rmet_5954"/>
    <dbReference type="HOGENOM" id="CLU_035706_0_0_4"/>
    <dbReference type="OMA" id="MHTIARS"/>
    <dbReference type="OrthoDB" id="1780129at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid pMOL30"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001584">
        <property type="entry name" value="Integrase_cat-core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025246">
        <property type="entry name" value="IS30-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13936">
        <property type="entry name" value="HTH_38"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="SSF53098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50994">
        <property type="entry name" value="INTEGRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Integrase catalytic" evidence="1">
        <location>
            <begin position="172"/>
            <end position="325"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF12813.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="336" mass="37773" checksum="0AB80D6DAA96B09B" modified="2005-04-26" version="1">MGQSYSQLGIEERNCSHRLLNEGCSLRQVARVAGRSPSTISREVARAGGHRSYDAASAQGMALRRRRRGPRKLAAGTALLKLVRAWLRVGYSPQQIAGRLRRMYAHQPEFHVSHETIYCVIYATPRGELRRELIGSLRKAHKERRSRGAGNDRRGKLQDMRSIHERPEEVFERLVPGHWEGDLIKGAGNRSAVATLVERKTRFVVLAKMDGCGAEAALEALTKKFRRVPLALRKSLTYDQGKEMAHHAELTRRVSTEVFFADPHSPWQRPSNENMNGLVREYLPKGDDLSVFSQGYLNGVPKALNYRPRAVLDFATPAEAMAEEINKLRTGVASQT</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-04-26" modified="2021-06-02" version="72" xmlns="http://uniprot.org/uniprot">
    <accession>Q58AR4</accession>
    <name>Q58AR4_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Antitoxin of a toxin/antitoxin system</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_6033</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="1 2">
        <name>pMOL30</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid pMOL30</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000354">
        <property type="protein sequence ID" value="ABF12892.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011229305.1">
        <property type="nucleotide sequence ID" value="NC_007971.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_145551.1">
        <property type="nucleotide sequence ID" value="NC_006466.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF12892">
        <property type="protein sequence ID" value="ABF12892"/>
        <property type="gene ID" value="Rmet_6033"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6033"/>
    <dbReference type="HOGENOM" id="CLU_179376_1_1_4"/>
    <dbReference type="OMA" id="MDKADLF"/>
    <dbReference type="OrthoDB" id="2064132at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid pMOL30"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019239">
        <property type="entry name" value="VapB_antitoxin"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09957">
        <property type="entry name" value="VapB_antitoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF12892.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="65" mass="7132" checksum="12358CDC2A598F5D" modified="2005-04-26" version="1">MRTTIALDDELLAKAQAYTGLEEKTALVREALKALIQREAAKRLANLGGSQPGIEGVPRRRQDTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-01-04" modified="2021-09-29" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q5NUZ8</accession>
    <name>Q5NUZ8_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">ChrE, involved in the processing of chromium-glutathione-complexes</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">chrE</name>
        <name evidence="2" type="ordered locus">Rmet_6200</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2 3">
        <name>pMOL28</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid pMOL28</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000355">
        <property type="protein sequence ID" value="ABF13059.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011239975.1">
        <property type="nucleotide sequence ID" value="NC_007972.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_161714.1">
        <property type="nucleotide sequence ID" value="NC_006525.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF13059">
        <property type="protein sequence ID" value="ABF13059"/>
        <property type="gene ID" value="Rmet_6200"/>
    </dbReference>
    <dbReference type="GeneID" id="60825783"/>
    <dbReference type="KEGG" id="rme:Rmet_6200"/>
    <dbReference type="HOGENOM" id="CLU_089574_16_0_4"/>
    <dbReference type="OMA" id="HGHEISQ"/>
    <dbReference type="OrthoDB" id="2056793at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid pMOL28"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.250.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001763">
        <property type="entry name" value="Rhodanese-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036873">
        <property type="entry name" value="Rhodanese-like_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00581">
        <property type="entry name" value="Rhodanese"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00450">
        <property type="entry name" value="RHOD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52821">
        <property type="entry name" value="SSF52821"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50206">
        <property type="entry name" value="RHODANESE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rhodanese" evidence="1">
        <location>
            <begin position="16"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF13059.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="113" mass="12246" checksum="19B132CE3F203775" modified="2005-01-04" version="1">MPNATSSLELAAALHGLNAPQLIDVRRKPAFDASEQMIAGAAWRNPDELGNWIATLDANRPVTVYCVHGHQVSQDCAALLEAVGFDATYLVGGFEDWIAGNHPTIRKPLRIEP</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-03-23" modified="2020-10-07" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>D3DXT4</accession>
    <name>D3DXT4_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_6496</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ADC45104.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_6496"/>
    <dbReference type="EnsemblBacteria" id="ADC45104">
        <property type="protein sequence ID" value="ADC45104"/>
        <property type="gene ID" value="Rmet_6496"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6496"/>
    <dbReference type="HOGENOM" id="CLU_2719418_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADC45104.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="72" mass="8150" checksum="9758EB77C3F4C9FB" modified="2010-03-23" version="1">MHAQTLLRATGQPLLFYSSTETTPDIVVVQPERHSHDTGRHPSMVAAPAHQFYHPRIPHNRQARAPNFLSLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-03-23" modified="2022-08-03" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>D3DXU5</accession>
    <name>D3DXU5_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Siroheme decarboxylase</fullName>
            <ecNumber evidence="5">4.1.1.111</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">nirH</name>
        <name evidence="8" type="ordered locus">Rmet_6507</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>2 H(+) + siroheme = 12,18-didecarboxysiroheme + 2 CO2</text>
            <dbReference type="Rhea" id="RHEA:19093"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:60052"/>
            <dbReference type="ChEBI" id="CHEBI:140497"/>
            <dbReference type="EC" id="4.1.1.111"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Porphyrin-containing compound metabolism.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the Ahb/Nir family.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.111" evidence="5"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ADC45115.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516795.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_6507"/>
    <dbReference type="EnsemblBacteria" id="ADC45115">
        <property type="protein sequence ID" value="ADC45115"/>
        <property type="gene ID" value="Rmet_6507"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6507"/>
    <dbReference type="eggNOG" id="COG1522">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_112007_0_1_4"/>
    <dbReference type="UniPathway" id="UPA00908">
        <property type="reaction ID" value="UER00884"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000485">
        <property type="entry name" value="AsnC-type_HTH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040523">
        <property type="entry name" value="AsnC_trans_reg2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17805">
        <property type="entry name" value="AsnC_trans_reg2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13404">
        <property type="entry name" value="HTH_AsnC-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0456">Lyase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH asnC-type" evidence="6">
        <location>
            <begin position="4"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="domain" description="AsnC_trans_reg2" evidence="7">
        <location>
            <begin position="64"/>
            <end position="151"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023444"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023457"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF13404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF17805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ADC45115.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="163" mass="18005" checksum="73A97926CE4188A0" modified="2010-03-23" version="1">MTHDSLDLALIRATQQGLPLVPEPFAAIGASLGIDESEVIARLQAMQTLGTLRRIGAVPNHYLLGWRANGMTVWDVDDAYTDTLGERVGALPFVSHCYRRTRHAPDWPYNLFAMVHAHTREEAQELVARIAALLGDACRAHDVLWSTRILKKTGLRLPATGNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-03-23" modified="2020-10-07" version="22" xmlns="http://uniprot.org/uniprot">
    <accession>D3DXW2</accession>
    <name>D3DXW2_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_6525</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ADC45132.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADC45132">
        <property type="protein sequence ID" value="ADC45132"/>
        <property type="gene ID" value="Rmet_6525"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6525"/>
    <dbReference type="HOGENOM" id="CLU_2828119_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADC45132.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="66" mass="7193" checksum="2824DA54AB0395FB" modified="2010-03-23" version="1">MDYRGRRSACFYGEAVCGRRGIPPAQSGTLTEIGPAAVEWQSRNTARQYALQASPGLMRCEASVSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-03-23" modified="2020-10-07" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>D3DXX1</accession>
    <name>D3DXX1_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_6534</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ADC45141.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_6534"/>
    <dbReference type="EnsemblBacteria" id="ADC45141">
        <property type="protein sequence ID" value="ADC45141"/>
        <property type="gene ID" value="Rmet_6534"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6534"/>
    <dbReference type="eggNOG" id="ENOG50337UK">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2685192_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADC45141.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="74" mass="8410" checksum="E6F71BF2A1241618" modified="2010-03-23" version="1">MTHMKLHLTGDRNQCPTCRLYFNSTSAFDKHRVGTWDDRRCLTVPEMEALGMAINKAGFWVGRPRSGNAIPSRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-03-23" modified="2020-10-07" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>D3DXY4</accession>
    <name>D3DXY4_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative sensory box protein/GGDEF family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_6547</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ADC45154.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_6547"/>
    <dbReference type="EnsemblBacteria" id="ADC45154">
        <property type="protein sequence ID" value="ADC45154"/>
        <property type="gene ID" value="Rmet_6547"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6547"/>
    <dbReference type="eggNOG" id="COG2199">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_11_16_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd01949">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000160">
        <property type="entry name" value="GGDEF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029787">
        <property type="entry name" value="Nucleotide_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00990">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00267">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55073">
        <property type="entry name" value="SSF55073"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00254">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50887">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GGDEF" evidence="1">
        <location>
            <begin position="108"/>
            <end position="239"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADC45154.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="239" mass="26039" checksum="868EA6A46611496D" modified="2010-03-23" version="1">MRSGDRSSATRAALAGTWPSQTVTVLSRRSAGESSARVVVKPSVALIRDKVGQAIGVVVVLHDVSLERAQAAQLAYEASHDPLTGLVNRPEFERRLETAILRARQEGCTHTLMYLDLDRFKAVNDTCGHAAGDELLCQIADLMRQQLRRSDTLARLGGDEFAVLLEHCLPSDGERVAESLRQAIAAFRFICRGHVFSVAVSVGLIRLDERTSNTEDALGAADVACYRAKRAGRNRVEVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-03-23" modified="2020-10-07" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>D3DXZ3</accession>
    <name>D3DXZ3_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_6557</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ADC45163.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADC45163">
        <property type="protein sequence ID" value="ADC45163"/>
        <property type="gene ID" value="Rmet_6557"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6557"/>
    <dbReference type="HOGENOM" id="CLU_2540127_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADC45163.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="83" mass="8571" checksum="C7DB5D9C987C90A8" modified="2010-03-23" version="1">MGLSAAAAFVDALVAAVPLLLLLLDEPQPQLEQPATASAAAPIVKPKSTRFMPSPPLAVKSPGDCTPDLHIRLPQTATPCQCT</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-03-23" modified="2020-10-07" version="22" xmlns="http://uniprot.org/uniprot">
    <accession>D3DY33</accession>
    <name>D3DY33_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_6602</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ADC45203.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_6602"/>
    <dbReference type="EnsemblBacteria" id="ADC45203">
        <property type="protein sequence ID" value="ADC45203"/>
        <property type="gene ID" value="Rmet_6602"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6602"/>
    <dbReference type="HOGENOM" id="CLU_2993482_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADC45203.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="57" mass="6502" checksum="399A450051DCFE17" modified="2010-03-23" version="1">MRTVSEHAGYPLQKCEFLAKTPILMGLRAQNATAIIDRPVFPTTHPDSRQFCIHTYQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-03-23" modified="2021-06-02" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>D3DY50</accession>
    <name>D3DY50_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_6621</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ADC45220.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518265.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_6621"/>
    <dbReference type="EnsemblBacteria" id="ADC45220">
        <property type="protein sequence ID" value="ADC45220"/>
        <property type="gene ID" value="Rmet_6621"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6621"/>
    <dbReference type="HOGENOM" id="CLU_2790987_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADC45220.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="68" mass="7559" checksum="F8FF71668BCC69CE" modified="2010-03-23" version="1">MRLETIRDPRSLVMGFIATAEHGVGVAHDTHMKVLGTYSVERNFTYDSLGCPFARGNRLLALVHRSAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-03-23" modified="2020-10-07" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>D3DY80</accession>
    <name>D3DY80_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_6651</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ADC45250.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_6651"/>
    <dbReference type="EnsemblBacteria" id="ADC45250">
        <property type="protein sequence ID" value="ADC45250"/>
        <property type="gene ID" value="Rmet_6651"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6651"/>
    <dbReference type="HOGENOM" id="CLU_2702163_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADC45250.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="73" mass="7667" checksum="8EF8B8AC7E6C7D1D" modified="2010-03-23" version="1">MAGMPRAARPGLAVSGALGRAIPPIVAELPLPASDAIRAVESCHRQKNDTPSAARNIGQLQAFLPHDNGMTRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>Q1L9I3</accession>
    <accession>Q1LIL1</accession>
    <name>Q1L9I3_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Protein involved in mobility of Tn6049</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">tnmB</name>
        <name evidence="1" type="ordered locus">Rmet_0322</name>
        <name evidence="2" type="ordered locus">Rmet_2001</name>
        <name evidence="3" type="ordered locus">Rmet_2007</name>
        <name evidence="4" type="ordered locus">Rmet_2404</name>
        <name evidence="5" type="ordered locus">Rmet_2553</name>
        <name evidence="6" type="ordered locus">Rmet_2838</name>
        <name evidence="7" type="ordered locus">Rmet_3143</name>
        <name evidence="8" type="ordered locus">Rmet_3932</name>
        <name evidence="9" type="ordered locus">Rmet_4425</name>
        <name evidence="10" type="ordered locus">Rmet_5481</name>
        <name evidence="11" type="ordered locus">Rmet_6181</name>
        <name evidence="12" type="ordered locus">Rmet_6334</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="8">
        <name>megaplasmid</name>
    </geneLocation>
    <geneLocation type="plasmid" evidence="13">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <geneLocation type="plasmid" evidence="12 13">
        <name>pMOL28</name>
    </geneLocation>
    <geneLocation type="plasmid" evidence="11 13">
        <name>pMOL30</name>
    </geneLocation>
    <reference evidence="12" key="1">
        <citation type="submission" date="2006-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of the chromosome of Ralstonia metallidurans CH34.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Copeland A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Barry K."/>
                <person name="Detter J.C."/>
                <person name="Glavina del Rio T."/>
                <person name="Hammon N."/>
                <person name="Israni S."/>
                <person name="Dalin E."/>
                <person name="Tice H."/>
                <person name="Martinez M."/>
                <person name="Goltsman E."/>
                <person name="Pitluck S."/>
                <person name="Schmutz J."/>
                <person name="Larimer F."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Kim E."/>
                <person name="Mergeay M."/>
                <person name="Benotmane M.A."/>
                <person name="Vallaeys T."/>
                <person name="Michaux A."/>
                <person name="Monchy S."/>
                <person name="Dunn J."/>
                <person name="McCorkle S."/>
                <person name="Taghavi S."/>
                <person name="van der Lelie D."/>
                <person name="Richardson P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">CH34</strain>
            <plasmid evidence="8">megaplasmid</plasmid>
            <plasmid evidence="12">pMOL28</plasmid>
            <plasmid evidence="11">pMOL30</plasmid>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="13">Plasmid megaplasmid CH34</plasmid>
            <plasmid evidence="13">Plasmid pMOL28</plasmid>
            <plasmid evidence="13">Plasmid pMOL30</plasmid>
        </source>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2010-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Benotmane R."/>
                <person name="Lapidus A."/>
                <person name="McCorkle S."/>
                <person name="Monchy S."/>
                <person name="Taghavi S."/>
                <person name="van der Lelie N."/>
                <person name="Dunn J."/>
                <person name="Leys N."/>
                <person name="Mergeay M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">CH34</strain>
            <plasmid evidence="8">megaplasmid</plasmid>
            <plasmid evidence="12">pMOL28</plasmid>
            <plasmid evidence="11">pMOL30</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07208.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08880.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08886.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09281.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09430.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09711.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10015.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10800.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11290.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12340.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP000354">
        <property type="protein sequence ID" value="ABF13040.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP000355">
        <property type="protein sequence ID" value="ABF13193.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0322"/>
    <dbReference type="EnsemblBacteria" id="ABF07208">
        <property type="protein sequence ID" value="ABF07208"/>
        <property type="gene ID" value="Rmet_0322"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF08880">
        <property type="protein sequence ID" value="ABF08880"/>
        <property type="gene ID" value="Rmet_2001"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF08886">
        <property type="protein sequence ID" value="ABF08886"/>
        <property type="gene ID" value="Rmet_2007"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF09281">
        <property type="protein sequence ID" value="ABF09281"/>
        <property type="gene ID" value="Rmet_2404"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF09430">
        <property type="protein sequence ID" value="ABF09430"/>
        <property type="gene ID" value="Rmet_2553"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF09711">
        <property type="protein sequence ID" value="ABF09711"/>
        <property type="gene ID" value="Rmet_2838"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF10015">
        <property type="protein sequence ID" value="ABF10015"/>
        <property type="gene ID" value="Rmet_3143"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF10800">
        <property type="protein sequence ID" value="ABF10800"/>
        <property type="gene ID" value="Rmet_3932"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF11290">
        <property type="protein sequence ID" value="ABF11290"/>
        <property type="gene ID" value="Rmet_4425"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF12340">
        <property type="protein sequence ID" value="ABF12340"/>
        <property type="gene ID" value="Rmet_5481"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF13040">
        <property type="protein sequence ID" value="ABF13040"/>
        <property type="gene ID" value="Rmet_6181"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF13193">
        <property type="protein sequence ID" value="ABF13193"/>
        <property type="gene ID" value="Rmet_6334"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0322"/>
    <dbReference type="KEGG" id="rme:Rmet_2001"/>
    <dbReference type="KEGG" id="rme:Rmet_2007"/>
    <dbReference type="KEGG" id="rme:Rmet_2404"/>
    <dbReference type="KEGG" id="rme:Rmet_2553"/>
    <dbReference type="KEGG" id="rme:Rmet_2838"/>
    <dbReference type="KEGG" id="rme:Rmet_3143"/>
    <dbReference type="KEGG" id="rme:Rmet_3932"/>
    <dbReference type="KEGG" id="rme:Rmet_4425"/>
    <dbReference type="KEGG" id="rme:Rmet_5481"/>
    <dbReference type="KEGG" id="rme:Rmet_6181"/>
    <dbReference type="KEGG" id="rme:Rmet_6334"/>
    <dbReference type="eggNOG" id="ENOG5032R4Q">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_123210_0_0_4"/>
    <dbReference type="OMA" id="DYAEQTR"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid pMOL28"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid pMOL30"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="12" id="KW-0614">Plasmid</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF07208.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF08880.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF08886.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF09281.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABF09430.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABF09711.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABF10015.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ABF10800.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ABF11290.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ABF12340.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ABF13040.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ABF13193.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="189" mass="21173" checksum="BE14C36ECD933F29" modified="2006-05-30" version="1">MSPLLCRAMKETSAVCHALLQTPAFFALLRRIDEDLMKVARDRGCCHCGSVLHSANYPRKPRGCPPAAHPDCNTRLSLCCAGCRKRMTPDSVRFLGRRVWLAIVLVLRSSRATGACLDGLPAISWATLKRWRQWWTETFPKTPVGRWLRGLIPPTPEPFIYPDCLLQSVEHDSEDERLAAVLLLLLGPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="57" xmlns="http://uniprot.org/uniprot">
    <accession>Q1L9M2</accession>
    <name>Q1L9M2_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized lipoprotein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_6295</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3 4">
        <name>pMOL28</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid pMOL28</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000355">
        <property type="protein sequence ID" value="ABF13154.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011514910.1">
        <property type="nucleotide sequence ID" value="NC_007972.2"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF13154">
        <property type="protein sequence ID" value="ABF13154"/>
        <property type="gene ID" value="Rmet_6295"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6295"/>
    <dbReference type="HOGENOM" id="CLU_1330096_0_0_4"/>
    <dbReference type="OMA" id="QGWQLAW"/>
    <dbReference type="OrthoDB" id="1608127at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid pMOL28"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018927">
        <property type="entry name" value="Pilus_synth_Q_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10671">
        <property type="entry name" value="TcpQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004192984" evidence="1">
        <location>
            <begin position="28"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="domain" description="TcpQ" evidence="2">
        <location>
            <begin position="108"/>
            <end position="186"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF10671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF13154.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="195" mass="20472" checksum="1CD4AA032CCF0A74" modified="2006-05-30" version="1">MHQSRSLSRMCRVLLAGAAVLAAYAHAAVPASTPFGADPSGNFEPSKPLAGGGWQLLSDRKAPKVDATTVAALAPTPAGAAPVASPVAAPPAAPVPVAAPVMANGQRDWSVSPGDGNYRLLIEKWARDAGWTMAPWEPDQDVQIEGADAFTGDFKTAVRRVLSATEMTDYSLKPCFYSNNLVRVVKLTTKCDPTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LA13</accession>
    <name>Q1LA13_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_6154</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="1 2">
        <name>pMOL30</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid pMOL30</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000354">
        <property type="protein sequence ID" value="ABF13013.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011514834.1">
        <property type="nucleotide sequence ID" value="NC_007971.2"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF13013">
        <property type="protein sequence ID" value="ABF13013"/>
        <property type="gene ID" value="Rmet_6154"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6154"/>
    <dbReference type="HOGENOM" id="CLU_1407745_0_0_4"/>
    <dbReference type="OrthoDB" id="1950881at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid pMOL30"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF13013.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="193" mass="21313" checksum="2A1C859EA3080E28" modified="2006-05-30" version="1">MDITLWPIPMTAQRREPPMEPGLACDWGGRRGPARSGQSTTTTTLHTIRSLVLKGIHRTMQRHTLYAYVDGTDLDDVAVAIERELVALADSSGWVASRPIVVNQKSMHAGSRPDDLPDWDLGVNLTLPEPEFEPTDWFADIERVVARLAQLHSKFGRDFVIGIADNASGVADDLFHIESETTDLGKLRMLIGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LAA7</accession>
    <name>Q1LAA7_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Plasmid replication protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">repA</name>
        <name evidence="2" type="ordered locus">Rmet_6060</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2 3">
        <name>pMOL30</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid pMOL30</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000354">
        <property type="protein sequence ID" value="ABF12919.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011514775.1">
        <property type="nucleotide sequence ID" value="NC_007971.2"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF12919">
        <property type="protein sequence ID" value="ABF12919"/>
        <property type="gene ID" value="Rmet_6060"/>
    </dbReference>
    <dbReference type="GeneID" id="60825056"/>
    <dbReference type="KEGG" id="rme:Rmet_6060"/>
    <dbReference type="HOGENOM" id="CLU_047931_0_0_4"/>
    <dbReference type="OMA" id="WLMRYDS"/>
    <dbReference type="OrthoDB" id="252556at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid pMOL30"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003887">
        <property type="term" value="F:DNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006270">
        <property type="term" value="P:DNA replication initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000525">
        <property type="entry name" value="Initiator_Rep_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01051">
        <property type="entry name" value="Rep_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rep_3" evidence="1">
        <location>
            <begin position="41"/>
            <end position="253"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01051"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF12919.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="445" mass="50391" checksum="71E61D5132C3034F" modified="2006-05-30" version="1">MSNLPKNIQEQLGFDIFNAVREELGPSTAITESTRDIGYRKTNVLIDVTPMSLVARRAINALWLTVSENPDLEHYDIELGYFKWLAKFETSNNVAHLKKALRECQSSSVQVSVERKGGGDPQWVSVPLLGSVGIAGGRVVFKVDEMIRRQLKDPKRYTYLSLRMSAALSQYAYILYERLGALRFMGTTNWLPIEEVRKWVNADAVKSLTEYKQFKRLVLDKNIAQINELTDLYVEYETKTAQGSRRVAAIRFKLRDNAEGKLVLDFAQPHELKDLYETLVKGFGLSTRQINEIISNREVNTDERIRAAIELVRYRVKVGGVKSPGAYLMTAIKEGWALSEMEREAAFGSPAEAVPSKPMVPVAEIEFARNLERDIEEGLAIFASMTDDERLAVLDGLYKETTFQMAARRQKVKARPDEAAIMARKALKVELAQYVKRQAEEQKAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="72" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LB44</accession>
    <name>Q1LB44_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Rmet_5773</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="5">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="5">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12632.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008652587.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5773"/>
    <dbReference type="EnsemblBacteria" id="ABF12632">
        <property type="protein sequence ID" value="ABF12632"/>
        <property type="gene ID" value="Rmet_5773"/>
    </dbReference>
    <dbReference type="GeneID" id="60823518"/>
    <dbReference type="KEGG" id="rme:Rmet_5773"/>
    <dbReference type="eggNOG" id="COG5126">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_137947_0_0_4"/>
    <dbReference type="OMA" id="PQIEAFM"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13202">
        <property type="entry name" value="EF-hand_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="SSF47473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0614">Plasmid</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193281" description="EF-hand domain-containing protein" evidence="2">
        <location>
            <begin position="24"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="3">
        <location>
            <begin position="37"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="23"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="41"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF12632.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="109" mass="11754" checksum="DD13A9C4A4A0BE6E" modified="2006-05-30" version="1">MSHRFAAAGAVFALLLLSATAGAQTQPQPQPPANAQEAKAKFTEKFNEADANHDGKLTREEAEAGMPHVAKDFDKIDAKKKGYVTQKQVGAYYAAKAKQRRAQDPGNLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="118" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LBA7</accession>
    <name>Q1LBA7_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Histidine kinase</fullName>
            <ecNumber evidence="2">2.7.13.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">bvgS1</name>
        <name evidence="11" type="ordered locus">Rmet_5710</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="12">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="12">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.</text>
            <dbReference type="EC" id="2.7.13.3"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.7.13.3" evidence="2"/>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12569.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5710"/>
    <dbReference type="EnsemblBacteria" id="ABF12569">
        <property type="protein sequence ID" value="ABF12569"/>
        <property type="gene ID" value="Rmet_5710"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5710"/>
    <dbReference type="eggNOG" id="COG2205">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_37_3_4"/>
    <dbReference type="OMA" id="NFGKHEA"/>
    <dbReference type="OrthoDB" id="1755994at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00082">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00130">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003661">
        <property type="entry name" value="HisK_dim/P"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036097">
        <property type="entry name" value="HisK_dim/P_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000014">
        <property type="entry name" value="PAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000700">
        <property type="entry name" value="PAS-assoc_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035965">
        <property type="entry name" value="PAS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013656">
        <property type="entry name" value="PAS_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004358">
        <property type="entry name" value="Sig_transdc_His_kin-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001638">
        <property type="entry name" value="Solute-binding_3/MltF_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00512">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08448">
        <property type="entry name" value="PAS_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00497">
        <property type="entry name" value="SBP_bac_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00344">
        <property type="entry name" value="BCTRLSENSOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00388">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00091">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00062">
        <property type="entry name" value="PBPb"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47384">
        <property type="entry name" value="SSF47384"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55785">
        <property type="entry name" value="SSF55785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="SSF55874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00229">
        <property type="entry name" value="sensory_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50113">
        <property type="entry name" value="PAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="11" id="KW-0614">Plasmid</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="11" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PAC" evidence="10">
        <location>
            <begin position="578"/>
            <end position="634"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="8">
        <location>
            <begin position="652"/>
            <end position="873"/>
        </location>
    </feature>
    <feature type="domain" description="Response regulatory" evidence="9">
        <location>
            <begin position="898"/>
            <end position="1015"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="281"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="6">
        <location>
            <position position="947"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ABF12569.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="1040" mass="112351" checksum="3D91A65609E6BE2D" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="64" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LBJ7</accession>
    <name>Q1LBJ7_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_5620</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12479.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011520026.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF12479">
        <property type="protein sequence ID" value="ABF12479"/>
        <property type="gene ID" value="Rmet_5620"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5620"/>
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    <dbReference type="OMA" id="PADRYDY"/>
    <dbReference type="OrthoDB" id="2091925at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004192985" evidence="2">
        <location>
            <begin position="21"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="22"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="69"/>
            <end position="87"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF12479.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="107" mass="11096" checksum="6974FE1ED1DED46D" modified="2006-05-30" version="1">MKALTIAVAVAVFVPGLAFAAGPASPSDRYDYDMHTTNKDGYVPDPARTSNKDGYTPDAARAGDKFDPYTQGANQSTSSALTDSKSTAKKSSKSHKKAPAAPTAATQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LBX7</accession>
    <name>Q1LBX7_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">4-hydroxybenzoate 3-monooxygenase, FAD-binding</fullName>
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        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">pobA</name>
        <name evidence="2" type="ordered locus">Rmet_5490</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EC" id="1.14.13.2" evidence="2"/>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12349.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519894.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5490"/>
    <dbReference type="EnsemblBacteria" id="ABF12349">
        <property type="protein sequence ID" value="ABF12349"/>
        <property type="gene ID" value="Rmet_5490"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5490"/>
    <dbReference type="eggNOG" id="COG0654">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_057691_0_0_4"/>
    <dbReference type="OMA" id="KRIWRAE"/>
    <dbReference type="OrthoDB" id="1770293at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018659">
        <property type="term" value="F:4-hydroxybenzoate 3-monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002938">
        <property type="entry name" value="FAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01494">
        <property type="entry name" value="FAD_binding_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="SSF51905"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD_binding_3" evidence="1">
        <location>
            <begin position="2"/>
            <end position="341"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF12349.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="392" mass="43535" checksum="94648D0F36A6AFB1" modified="2006-05-30" version="1">MRTQVGIIGAGPAGLLLSHLLHLKGIDSVVIESRTREEIESTIRAGVLEQGTMDLLQDVGLGNRMVAEGALHQGFELTFEGERHRIDLTDLTGHAITVYAQHEVIKDLVAARVAAGGKLVFGVSNTSLHDVESTKPSIRYEKDDDTCEIHCDFVIGCDGSQGPSRSAIPEAVRKDHQRLYPFGWFGILVEAPPSSDELIYARHDRGFALISTRSPTVQRMYFQCDPRDSVENWSDDRIWSELHARLDQADGWRVTEGRIFQKNIVGMRSFVSNVMQHGRLFLAGDSAHIVPPTGAKGMNLAVNDVKILAEGLDSFYKNGTEDKLNAYTATALQRIWRAEHFSWWMTSMLHRFADATPFDQQLQVSELRYVTSSRAGATALAENYVGMVGLSH</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="108" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LC22</accession>
    <name>Q1LC22_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Glycerol kinase</fullName>
            <ecNumber evidence="4">2.7.1.30</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Rmet_5445</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="5">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="5">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the FGGY kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.30" evidence="4"/>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12304.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519851.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5445"/>
    <dbReference type="EnsemblBacteria" id="ABF12304">
        <property type="protein sequence ID" value="ABF12304"/>
        <property type="gene ID" value="Rmet_5445"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5445"/>
    <dbReference type="eggNOG" id="COG0554">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009281_2_3_4"/>
    <dbReference type="OMA" id="RSPYLWA"/>
    <dbReference type="OrthoDB" id="233605at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004370">
        <property type="term" value="F:glycerol kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000577">
        <property type="entry name" value="Carb_kinase_FGGY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018485">
        <property type="entry name" value="Carb_kinase_FGGY_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018483">
        <property type="entry name" value="Carb_kinase_FGGY_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018484">
        <property type="entry name" value="Carb_kinase_FGGY_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02782">
        <property type="entry name" value="FGGY_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00370">
        <property type="entry name" value="FGGY_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000538">
        <property type="entry name" value="GlpK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="SSF53067"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00445">
        <property type="entry name" value="FGGY_KINASES_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 4" id="KW-0418">Kinase</keyword>
    <keyword evidence="4" id="KW-0614">Plasmid</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="FGGY_N" evidence="2">
        <location>
            <begin position="6"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="domain" description="FGGY_C" evidence="3">
        <location>
            <begin position="261"/>
            <end position="452"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU003733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF02782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF12304.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="506" mass="54685" checksum="9DA9686831EDD7B1" modified="2006-05-30" version="1">MKNRNVILAIDEGTSGTRAAVVASDGHVSCQQYTTLQVDSPRPGVVEQDANVLLEKTLEVCRATLAEAERNDLCIVALAIATQRSTGVLWDTVTGRALVPAMVWQDTRYAADLDQMASTWDPRLRATVGRPAGVRSAYLWAARHLRETRAVADAWRDRRLAFGTVDSWLLWHLSTERACVTTPTNATSCSAYVLGEHRYLTEWVEALGFPQELLPELRQDADDFGRTRRDLLGVDVPILACAGDQLAGAVGLGCLDAGQSMCLHGTGSFVDLVIGPDLPERSGQSDSTLTMTARRFEGVSHFSVETFVATTGSALNWVCDKLHWFESAKQISALAASVPSSRGVTFIPALTGLRVPQMRPEARAALNGVSMATTQAEIAYAILEGIAHSVASCIDANRDIAGVPVRDLVVGGGLSGSDALLQMQADLTGLPIHRMQETDRASLRGIAYLAGASGLMWDSLQQARTTTLPDAVFMPRIGDDEREERRALWHARVASELSHVQAFAAH</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="107" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LC75</accession>
    <name>Q1LC75_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Two component transcriptional regulator, winged helix family ygiX</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Rmet_5392</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="6">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="6">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12251.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519797.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5392"/>
    <dbReference type="EnsemblBacteria" id="ABF12251">
        <property type="protein sequence ID" value="ABF12251"/>
        <property type="gene ID" value="Rmet_5392"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5392"/>
    <dbReference type="eggNOG" id="COG0745">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_30_1_4"/>
    <dbReference type="OMA" id="EMGSDDY"/>
    <dbReference type="OrthoDB" id="1020672at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00383">
        <property type="entry name" value="trans_reg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001867">
        <property type="entry name" value="OmpR/PhoB-type_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039420">
        <property type="entry name" value="WalR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48111">
        <property type="entry name" value="PTHR48111"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00486">
        <property type="entry name" value="Trans_reg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00862">
        <property type="entry name" value="Trans_reg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51755">
        <property type="entry name" value="OMPR_PHOB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="5" id="KW-0614">Plasmid</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Response regulatory" evidence="3">
        <location>
            <begin position="2"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="domain" description="OmpR/PhoB-type" evidence="4">
        <location>
            <begin position="124"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="OmpR/PhoB-type" evidence="2">
        <location>
            <begin position="124"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="1">
        <location>
            <position position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABF12251.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="219" mass="24329" checksum="71E3D8492409D09E" modified="2006-05-30" version="1">MRVLLIEDEPWLGQAVRDQIEKDGHPTDFVANLADARRHLAAATYDLILLDLLLPDGRGLDFLRELRASGSNVPIIILTALDQVSNRIAGLNAGADDYLVKPFDLSELSARVSAVARRYAGNPNPLVNLGDLRIDTAGHSVMRGETRVDLTASEWALLEALIQHPGMIVSRARLEERLYSFNEEVGSNTIEVHMSRLRKKLGADVIETLRGLGYRLRRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LC95</accession>
    <name>Q1LC95_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">tRNA_edit domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_5372</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12231.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5372"/>
    <dbReference type="EnsemblBacteria" id="ABF12231">
        <property type="protein sequence ID" value="ABF12231"/>
        <property type="gene ID" value="Rmet_5372"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5372"/>
    <dbReference type="eggNOG" id="COG2606">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_094875_2_1_4"/>
    <dbReference type="OMA" id="ITHPYSE"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002161">
        <property type="term" value="F:aminoacyl-tRNA editing activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.960.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036754">
        <property type="entry name" value="YbaK/aa-tRNA-synt-asso_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007214">
        <property type="entry name" value="YbaK/aa-tRNA-synth-assoc-dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04073">
        <property type="entry name" value="tRNA_edit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55826">
        <property type="entry name" value="SSF55826"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="tRNA_edit" evidence="1">
        <location>
            <begin position="45"/>
            <end position="148"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF04073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF12231.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="162" mass="17956" checksum="2699E36986111A52" modified="2006-05-30" version="1">MRGTRTTVAIGASEDAIMAMASTLARCLHNRQSRFDTVDRPCSAGDRLAKTVLLEDQRGYVAAVIPSTHHLKLSEIRAQTGRKLSLARDCGVREVFRDCSRGAIPPVAMAYGTQTWLDDSLLSHPDVYFEAGDHRALVHMSTDQFVDLMADAQRGHFSHRDM</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-11-25" modified="2022-08-03" version="95" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LC96</accession>
    <name>KATG_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Catalase-peroxidase</fullName>
            <shortName evidence="1">CP</shortName>
            <ecNumber evidence="1">1.11.1.21</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Peroxidase/catalase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">katG</name>
        <name type="ordered locus">Rmet_5371</name>
    </gene>
    <organism>
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Bifunctional enzyme with both catalase and broad-spectrum peroxidase activity.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>AH2 + H2O2 = A + 2 H2O</text>
            <dbReference type="Rhea" id="RHEA:30275"/>
            <dbReference type="ChEBI" id="CHEBI:13193"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:17499"/>
            <dbReference type="EC" id="1.11.1.21"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>2 H2O2 = 2 H2O + O2</text>
            <dbReference type="Rhea" id="RHEA:20309"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="EC" id="1.11.1.21"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>heme b</name>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
        </cofactor>
        <text evidence="1">Binds 1 heme b (iron(II)-protoporphyrin IX) group per dimer.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer or homotetramer.</text>
    </comment>
    <comment type="PTM">
        <text evidence="1">Formation of the three residue Trp-Tyr-Met cross-link is important for the catalase, but not the peroxidase activity of the enzyme.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the peroxidase family. Peroxidase/catalase subfamily.</text>
    </comment>
    <dbReference type="EC" id="1.11.1.21" evidence="1"/>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12230.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519777.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q1LC96"/>
    <dbReference type="SMR" id="Q1LC96"/>
    <dbReference type="STRING" id="266264.Rmet_5371"/>
    <dbReference type="PeroxiBase" id="2326">
        <property type="entry name" value="RmCP01"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF12230">
        <property type="protein sequence ID" value="ABF12230"/>
        <property type="gene ID" value="Rmet_5371"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5371"/>
    <dbReference type="eggNOG" id="COG0376">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025424_2_0_4"/>
    <dbReference type="OMA" id="MILAGNC"/>
    <dbReference type="OrthoDB" id="49441at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004096">
        <property type="term" value="F:catalase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042744">
        <property type="term" value="P:hydrogen peroxide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006979">
        <property type="term" value="P:response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01961">
        <property type="entry name" value="Catal_peroxid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000763">
        <property type="entry name" value="Catalase_peroxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002016">
        <property type="entry name" value="Haem_peroxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010255">
        <property type="entry name" value="Haem_peroxidase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019794">
        <property type="entry name" value="Peroxidases_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019793">
        <property type="entry name" value="Peroxidases_heam-ligand_BS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30555">
        <property type="entry name" value="PTHR30555"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00141">
        <property type="entry name" value="peroxidase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00460">
        <property type="entry name" value="BPEROXIDASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00458">
        <property type="entry name" value="PEROXIDASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48113">
        <property type="entry name" value="SSF48113"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00198">
        <property type="entry name" value="cat_per_HPI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00435">
        <property type="entry name" value="PEROXIDASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00436">
        <property type="entry name" value="PEROXIDASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50873">
        <property type="entry name" value="PEROXIDASE_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0349">Heme</keyword>
    <keyword id="KW-0376">Hydrogen peroxide</keyword>
    <keyword id="KW-0408">Iron</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0560">Oxidoreductase</keyword>
    <keyword id="KW-0575">Peroxidase</keyword>
    <keyword id="KW-0614">Plasmid</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000354878" description="Catalase-peroxidase">
        <location>
            <begin position="1"/>
            <end position="726"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="335"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="1">
        <location>
            <position position="92"/>
        </location>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="1">
        <location>
            <position position="255"/>
        </location>
        <ligand>
            <name>heme b</name>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="1">
        <location>
            <position position="88"/>
        </location>
    </feature>
    <feature type="cross-link" description="Tryptophyl-tyrosyl-methioninium (Trp-Tyr) (with M-240)" evidence="1">
        <location>
            <begin position="91"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="cross-link" description="Tryptophyl-tyrosyl-methioninium (Tyr-Met) (with W-91)" evidence="1">
        <location>
            <begin position="214"/>
            <end position="240"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="726" mass="79482" checksum="BDAFC89CC1242E08" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="104" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LCE4</accession>
    <name>Q1LCE4_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Transcriptional regulator two component heavy metal regulatory response ZniR</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">zniR</name>
        <name evidence="5" type="ordered locus">Rmet_5323</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="6">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="6">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12182.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519726.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5323"/>
    <dbReference type="EnsemblBacteria" id="ABF12182">
        <property type="protein sequence ID" value="ABF12182"/>
        <property type="gene ID" value="Rmet_5323"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5323"/>
    <dbReference type="eggNOG" id="COG2197">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_90_4_4"/>
    <dbReference type="OMA" id="IPDMIIC"/>
    <dbReference type="OrthoDB" id="1755994at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06170">
        <property type="entry name" value="LuxR_C_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016032">
        <property type="entry name" value="Sig_transdc_resp-reg_C-effctor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000792">
        <property type="entry name" value="Tscrpt_reg_LuxR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039420">
        <property type="entry name" value="WalR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48111">
        <property type="entry name" value="PTHR48111"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00196">
        <property type="entry name" value="GerE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00038">
        <property type="entry name" value="HTHLUXR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00421">
        <property type="entry name" value="HTH_LUXR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46894">
        <property type="entry name" value="SSF46894"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00622">
        <property type="entry name" value="HTH_LUXR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50043">
        <property type="entry name" value="HTH_LUXR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="5" id="KW-0614">Plasmid</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Response regulatory" evidence="4">
        <location>
            <begin position="11"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="domain" description="HTH luxR-type" evidence="3">
        <location>
            <begin position="137"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="2">
        <location>
            <position position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABF12182.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="206" mass="22556" checksum="6A517755751D1529" modified="2006-05-30" version="1">MVTSADNTPHQILCIEDDAETATLIAEELTERGFAVTVAHDGQEGFAAILRMPPDLVLCDVGMPVMDGFEVLAKLRALAPRYAEVPFVFLTALAQRDAELKGRRLGADDYVAKPVDFDILESIIEARLSRAVKPQQRTPQNVTLSEREIEALSWSARGKTSGEIATILGLSKRTVDFHIDRAREKLGVATRVEAVVRAATEGFIKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="64" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LCF9</accession>
    <name>Q1LCF9_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">zf-TFIIB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_5308</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12167.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008651884.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5308"/>
    <dbReference type="EnsemblBacteria" id="ABF12167">
        <property type="protein sequence ID" value="ABF12167"/>
        <property type="gene ID" value="Rmet_5308"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5308"/>
    <dbReference type="eggNOG" id="COG3809">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_147819_0_1_4"/>
    <dbReference type="OMA" id="VMTERQG"/>
    <dbReference type="OrthoDB" id="1847417at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027392">
        <property type="entry name" value="TF_Znf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13453">
        <property type="entry name" value="zf-TFIIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="zf-TFIIB" evidence="2">
        <location>
            <begin position="2"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="43"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="62"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13453"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF12167.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="99" mass="11061" checksum="8EAF240BE1357B97" modified="2006-05-30" version="1">MKCPTCPDSVLIMADRQGVEIDYCPRCRGVWLDRGELDKILERSAMATPVSQASAPRERPASPAYHGDDHGRGGHDGYGSRNGHHGNKRRKSWLNELFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LCJ8</accession>
    <name>Q1LCJ8_CUPMC</name>
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        <submittedName>
            <fullName evidence="7">Sodium/sulphate symporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Rmet_5269</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="8">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="8">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12128.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5269"/>
    <dbReference type="EnsemblBacteria" id="ABF12128">
        <property type="protein sequence ID" value="ABF12128"/>
        <property type="gene ID" value="Rmet_5269"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5269"/>
    <dbReference type="eggNOG" id="COG0471">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005170_7_1_4"/>
    <dbReference type="OMA" id="VILWVTE"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001898">
        <property type="entry name" value="SLC13A/DASS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00939">
        <property type="entry name" value="Na_sulph_symp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0614">Plasmid</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="79"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="103"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="129"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="153"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="187"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="213"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="238"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="280"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="329"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="352"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="384"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="425"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="500"/>
            <end position="523"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="16"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="32"/>
            <end position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABF12128.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="530" mass="55131" checksum="19E3F76FF4DE8815" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-11-03" modified="2022-08-03" version="95" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LCQ8</accession>
    <name>HOA2_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">4-hydroxy-2-oxovalerate aldolase 2</fullName>
            <shortName evidence="1">HOA 2</shortName>
            <ecNumber evidence="1">4.1.3.39</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">4-hydroxy-2-keto-pentanoic acid aldolase 2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">4-hydroxy-2-oxopentanoate aldolase 2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="ordered locus">Rmet_5209</name>
    </gene>
    <organism>
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(S)-4-hydroxy-2-oxopentanoate = acetaldehyde + pyruvate</text>
            <dbReference type="Rhea" id="RHEA:22624"/>
            <dbReference type="ChEBI" id="CHEBI:15343"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:73143"/>
            <dbReference type="EC" id="4.1.3.39"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the 4-hydroxy-2-oxovalerate aldolase family.</text>
    </comment>
    <dbReference type="EC" id="4.1.3.39" evidence="1"/>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12068.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519614.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q1LCQ8"/>
    <dbReference type="SMR" id="Q1LCQ8"/>
    <dbReference type="STRING" id="266264.Rmet_5209"/>
    <dbReference type="EnsemblBacteria" id="ABF12068">
        <property type="protein sequence ID" value="ABF12068"/>
        <property type="gene ID" value="Rmet_5209"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5209"/>
    <dbReference type="eggNOG" id="COG0119">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_049173_0_0_4"/>
    <dbReference type="OMA" id="WARENGM"/>
    <dbReference type="OrthoDB" id="840579at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008701">
        <property type="term" value="F:4-hydroxy-2-oxovalerate aldolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030145">
        <property type="term" value="F:manganese ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019439">
        <property type="term" value="P:aromatic compound catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd07943">
        <property type="entry name" value="DRE_TIM_HOA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01656">
        <property type="entry name" value="HOA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017629">
        <property type="entry name" value="4OH_2_O-val_aldolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012425">
        <property type="entry name" value="DmpG_comm"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035685">
        <property type="entry name" value="DRE_TIM_HOA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000891">
        <property type="entry name" value="PYR_CT"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07836">
        <property type="entry name" value="DmpG_comm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00682">
        <property type="entry name" value="HMGL-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03217">
        <property type="entry name" value="4OH_2_O_val_ald"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50991">
        <property type="entry name" value="PYR_CT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0058">Aromatic hydrocarbons catabolism</keyword>
    <keyword id="KW-0456">Lyase</keyword>
    <keyword id="KW-0464">Manganese</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0614">Plasmid</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000387889" description="4-hydroxy-2-oxovalerate aldolase 2">
        <location>
            <begin position="1"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="domain" description="Pyruvate carboxyltransferase" evidence="1">
        <location>
            <begin position="6"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="1">
        <location>
            <position position="18"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="14"/>
            <end position="15"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="15"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="168"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
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        <location>
            <position position="197"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="197"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="199"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="288"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="1">
        <location>
            <position position="14"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01656"/>
        </source>
    </evidence>
    <sequence length="345" mass="36821" checksum="91591FB626827C6D" modified="2006-05-30" version="1">MTDKKLYISDVTLRDGSHAIRHQYSVPQVRAIARALDAAGVDSIEVAHGDGLAGSSFNYGFGAHTDVEWIAAVAESVQRAAVATLLLPGIGTVHDLREAYAAGARVVRVATHCTEADTARQHIETARSMGMNVAGFLMMSHMIPPDRLAGQAKLMESYGAHCVYVVDSGGALTMDGVRARFRAFKDVLDPKTETGMHAHHNLSLGVANSIVAVEEGCDRIDASLAGMGAGAGNAPLEVFIAAAERMGWHHGCDLYQLMDAADDIVRPLQDRPVRVDRETLALGYAGVYSSFLRHAESAAGKYGLKTVDILVELGRRRMVGGQEDMIVDVALDLQRSGGAKSREAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LD12</accession>
    <name>Q1LD12_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Putative nicotinamide phosphoribosyltransferase (NAPRTase)</fullName>
            <ecNumber evidence="5">2.4.2.12</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Rmet_5105</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="6">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="6">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the NAPRTase family.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.12" evidence="5"/>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11964.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519511.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="SMR" id="Q1LD12"/>
    <dbReference type="STRING" id="266264.Rmet_5105"/>
    <dbReference type="EnsemblBacteria" id="ABF11964">
        <property type="protein sequence ID" value="ABF11964"/>
        <property type="gene ID" value="Rmet_5105"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5105"/>
    <dbReference type="eggNOG" id="COG1488">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012550_2_0_4"/>
    <dbReference type="OMA" id="TFGFAMK"/>
    <dbReference type="OrthoDB" id="241792at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047280">
        <property type="term" value="F:nicotinamide phosphoribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004514">
        <property type="term" value="F:nicotinate-nucleotide diphosphorylase (carboxylating) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009435">
        <property type="term" value="P:NAD biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01569">
        <property type="entry name" value="PBEF_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041529">
        <property type="entry name" value="DUF5598"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041525">
        <property type="entry name" value="N/Namide_PRibTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016471">
        <property type="entry name" value="Nicotinamide_PRibTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036068">
        <property type="entry name" value="Nicotinate_pribotase-like_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43816">
        <property type="entry name" value="PTHR43816"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18127">
        <property type="entry name" value="NAMPT_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04095">
        <property type="entry name" value="NAPRTase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005943">
        <property type="entry name" value="NMPRT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51690">
        <property type="entry name" value="SSF51690"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="5" id="KW-0614">Plasmid</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="NAMPT_N" evidence="4">
        <location>
            <begin position="16"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="domain" description="NAPRTase" evidence="3">
        <location>
            <begin position="179"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="region of interest" description="Nicotinamide ribonucleotide binding" evidence="2">
        <location>
            <begin position="299"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="region of interest" description="Nicotinamide ribonucleotide binding" evidence="2">
        <location>
            <begin position="340"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="187"/>
        </location>
        <ligand>
            <name>diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="210"/>
        </location>
        <ligand>
            <name>beta-nicotinamide D-ribonucleotide</name>
            <dbReference type="ChEBI" id="CHEBI:14649"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="236"/>
        </location>
        <ligand>
            <name>diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="299"/>
        </location>
        <ligand>
            <name>diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="371"/>
        </location>
        <ligand>
            <name>beta-nicotinamide D-ribonucleotide</name>
            <dbReference type="ChEBI" id="CHEBI:14649"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="379"/>
        </location>
        <ligand>
            <name>beta-nicotinamide D-ribonucleotide</name>
            <dbReference type="ChEBI" id="CHEBI:14649"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010897"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR005943-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF04095"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF18127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABF11964.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="477" mass="53002" checksum="B95A756EE8CA2725" modified="2006-05-30" version="1">MQNDLRRLSSILSNPILNTDSYKASHYLQYPQGTTAMFSYIESRGGRYDRTLFFGLQMLIKEYLCQPITAAMIDEARDFFSAHGEPFNESGWRYIVSRYDGYLPVRIRAVQEGAVIPTQNVLVTVECDDPEVFWLASYIETLLLRVWYPVTVATQSWHLRQLVRRYLERTSDDLGQLPFKVHDFGARGVSSAESAAIGGAAHLVSFLGSDTVLGVLAANTYYNKPMAAFSVPAAEHSTITAWGRAGEIDAYRNMLRQFGRPGAIVSVVSDSYDLFAALRMWGTELRQAVIDSGATLVIRPDSGDPRTIVLQTIGALDEAFGSVINGKGYRVLNHVRVIQGDGVNAESIEVILAALEAAGYAADNIVFGIGGALLQQLNRDTQRFAMKCSAVKVDDAWRNVRKDPVTDAGKRSKQGRLTLLRNRRTGDYQTVTFPLAWDARRIEGEWEDALETVFDGGKLLVDVSLDEVRARAEAYED</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LD53</accession>
    <name>Q1LD53_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Extra-cytoplasmic solute receptor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">bugT</name>
        <name evidence="4" type="ordered locus">Rmet_5061</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="5">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="5">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Periplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the UPF0065 (bug) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11923.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5061"/>
    <dbReference type="EnsemblBacteria" id="ABF11923">
        <property type="protein sequence ID" value="ABF11923"/>
        <property type="gene ID" value="Rmet_5061"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5061"/>
    <dbReference type="eggNOG" id="COG3181">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045683_0_0_4"/>
    <dbReference type="OMA" id="WPAKPIQ"/>
    <dbReference type="OrthoDB" id="447734at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042597">
        <property type="term" value="C:periplasmic space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005064">
        <property type="entry name" value="BUG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042100">
        <property type="entry name" value="Bug_dom1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42928">
        <property type="entry name" value="PTHR42928"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03401">
        <property type="entry name" value="TctC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017082">
        <property type="entry name" value="YflP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-0675">Receptor</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193361" evidence="3">
        <location>
            <begin position="36"/>
            <end position="335"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF11923.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="335" mass="34696" checksum="DA988D85EFAD0EEF" modified="2006-05-30" version="1">MTRMTTGKMSLTARIALRVLLATAAIAALPLGAHAADAWPSRPIQLLIPYPPGGSADLLARPLGAKLQERLGQPIVLDYRPGAGGTIATQALARSKPDGYTLIMVLAAHAINPSLYPKLPYDTRKDFAPVSLVANLPLILAGSPSLKARNVPELIAAAKAAPGTITFGSAGNGNTGHLAGELFDSMAGVKMTHVPYKGSAQVVNAMLAGDIQLTFDSISTSMPQIRAGRMTALAVTSAKRAAMAPDVPTLAEAGVPGFDINGWYAILAPAGTPRAVVDKLSSEIAAVLTQPELRSQLAANGYEPVGSTPAALGAHIDAELVRWNKVVRDAGVKLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="111" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LD69</accession>
    <name>Q1LD69_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Formimidoylglutamase</fullName>
            <ecNumber evidence="5">3.5.3.8</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Formiminoglutamase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="5">Formiminoglutamate hydrolase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5 8" type="primary">hutG</name>
        <name evidence="8" type="ordered locus">Rmet_5045</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="9">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="9">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Catalyzes the conversion of N-formimidoyl-L-glutamate to L-glutamate and formamide.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>H2O + N-formimidoyl-L-glutamate = formamide + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:22492"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16397"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:58928"/>
            <dbReference type="EC" id="3.5.3.8"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="5">Binds 2 manganese ions per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="5">Amino-acid degradation; L-histidine degradation into L-glutamate; L-glutamate from N-formimidoyl-L-glutamate (hydrolase route): step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5 6 7">Belongs to the arginase family.</text>
    </comment>
    <dbReference type="EC" id="3.5.3.8" evidence="5"/>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11907.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5045"/>
    <dbReference type="EnsemblBacteria" id="ABF11907">
        <property type="protein sequence ID" value="ABF11907"/>
        <property type="gene ID" value="Rmet_5045"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5045"/>
    <dbReference type="eggNOG" id="COG0010">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039478_2_0_4"/>
    <dbReference type="OMA" id="GTMFRQI"/>
    <dbReference type="UniPathway" id="UPA00379">
        <property type="reaction ID" value="UER00552"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050415">
        <property type="term" value="F:formimidoylglutamase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030145">
        <property type="term" value="F:manganese ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019556">
        <property type="term" value="P:histidine catabolic process to glutamate and formamide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019557">
        <property type="term" value="P:histidine catabolic process to glutamate and formate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00737">
        <property type="entry name" value="Formimidoylglutam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005923">
        <property type="entry name" value="HutG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006035">
        <property type="entry name" value="Ureohydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023696">
        <property type="entry name" value="Ureohydrolase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020855">
        <property type="entry name" value="Ureohydrolase_Mn_BS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11358">
        <property type="entry name" value="PTHR11358"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00491">
        <property type="entry name" value="Arginase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00116">
        <property type="entry name" value="ARGINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52768">
        <property type="entry name" value="SSF52768"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01227">
        <property type="entry name" value="hutG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01053">
        <property type="entry name" value="ARGINASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51409">
        <property type="entry name" value="ARGINASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0369">Histidine metabolism</keyword>
    <keyword evidence="2 5 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 5" id="KW-0464">Manganese</keyword>
    <keyword evidence="1 5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0614">Plasmid</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="5">
        <location>
            <position position="140"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="169"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="171"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="173"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="260"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="262"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00742"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ABF11907.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="329" mass="35115" checksum="07548DA3A9554293" modified="2006-05-30" version="1">MFRETDVRQMTALTQAEALDAVWQGRADTGERGDTRRLFNIVQPLAVAASDDLAGATVLIGFACDAGVRRNQGRVGAADGPRGIRRALASLPVHDVAALYDAGDVRCEGDALEVTQRALSEVVRAQLDRGALPIVLGGGHEVAWGTWQGLRAHLDTAGDAGRVLILNLDAHFDLRTARPGSSGTPFDQIAEACRAAGQPFDYACLGVSRLGNTASLFAHAQALGVRYVEDTEMQERHLDHRLAEIDAMLAQAAHVYLTIDLDVLPAAVMPGVSAPAAYGVPISVIEAIVTHVRGSGKLRAADLAEFNPQFDRDGQSARVAARLVYRLIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="92" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LDD1</accession>
    <name>Q1LDD1_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Propeptide, PepSY amd peptidase M4 (Tn6048)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">mmfA</name>
        <name evidence="3" type="ordered locus">Rmet_2999</name>
        <name evidence="4" type="ordered locus">Rmet_4034</name>
        <name evidence="5" type="ordered locus">Rmet_4983</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="5">
        <name>megaplasmid</name>
    </geneLocation>
    <geneLocation type="plasmid" evidence="6">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="5" key="1">
        <citation type="submission" date="2006-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of the chromosome of Ralstonia metallidurans CH34.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Copeland A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Barry K."/>
                <person name="Detter J.C."/>
                <person name="Glavina del Rio T."/>
                <person name="Hammon N."/>
                <person name="Israni S."/>
                <person name="Dalin E."/>
                <person name="Tice H."/>
                <person name="Martinez M."/>
                <person name="Goltsman E."/>
                <person name="Pitluck S."/>
                <person name="Schmutz J."/>
                <person name="Larimer F."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Kim E."/>
                <person name="Mergeay M."/>
                <person name="Benotmane M.A."/>
                <person name="Vallaeys T."/>
                <person name="Michaux A."/>
                <person name="Monchy S."/>
                <person name="Dunn J."/>
                <person name="McCorkle S."/>
                <person name="Taghavi S."/>
                <person name="van der Lelie D."/>
                <person name="Richardson P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">CH34</strain>
            <plasmid evidence="5">megaplasmid</plasmid>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="6">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2010-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Benotmane R."/>
                <person name="Lapidus A."/>
                <person name="McCorkle S."/>
                <person name="Monchy S."/>
                <person name="Taghavi S."/>
                <person name="van der Lelie N."/>
                <person name="Dunn J."/>
                <person name="Leys N."/>
                <person name="Mergeay M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">CH34</strain>
            <plasmid evidence="5">megaplasmid</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09872.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10902.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11845.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517525.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2999"/>
    <dbReference type="EnsemblBacteria" id="ABF09872">
        <property type="protein sequence ID" value="ABF09872"/>
        <property type="gene ID" value="Rmet_2999"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF10902">
        <property type="protein sequence ID" value="ABF10902"/>
        <property type="gene ID" value="Rmet_4034"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF11845">
        <property type="protein sequence ID" value="ABF11845"/>
        <property type="gene ID" value="Rmet_4983"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2999"/>
    <dbReference type="KEGG" id="rme:Rmet_4034"/>
    <dbReference type="KEGG" id="rme:Rmet_4983"/>
    <dbReference type="eggNOG" id="COG3212">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_173389_0_0_4"/>
    <dbReference type="OMA" id="YENSKQG"/>
    <dbReference type="OrthoDB" id="1924559at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025711">
        <property type="entry name" value="PepSY"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03413">
        <property type="entry name" value="PepSY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0614">Plasmid</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5013530857" evidence="1">
        <location>
            <begin position="24"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="domain" description="PepSY" evidence="2">
        <location>
            <begin position="39"/>
            <end position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF03413"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF09872.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF10902.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABF11845.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="111" mass="11887" checksum="7B6BA381E8528E8D" modified="2006-05-30" version="1">MYRYTKLSLLAVAIAATGAVAYAANGGMENDALAISKAKIPLTQAVTVAEQHANGKASRAEYENSKQGWVYDVEVVSGAKVFDVRVDADKGTVISSTEDKADHDDDHDKRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="130" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LDD7</accession>
    <name>Q1LDD7_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Histidine kinase</fullName>
            <ecNumber evidence="2">2.7.13.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">mmfS</name>
        <name evidence="9" type="ordered locus">Rmet_3005</name>
        <name evidence="10" type="ordered locus">Rmet_4040</name>
        <name evidence="11" type="ordered locus">Rmet_4977</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="11">
        <name>megaplasmid</name>
    </geneLocation>
    <geneLocation type="plasmid" evidence="12">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="11" key="1">
        <citation type="submission" date="2006-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of the chromosome of Ralstonia metallidurans CH34.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Copeland A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Barry K."/>
                <person name="Detter J.C."/>
                <person name="Glavina del Rio T."/>
                <person name="Hammon N."/>
                <person name="Israni S."/>
                <person name="Dalin E."/>
                <person name="Tice H."/>
                <person name="Martinez M."/>
                <person name="Goltsman E."/>
                <person name="Pitluck S."/>
                <person name="Schmutz J."/>
                <person name="Larimer F."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Kim E."/>
                <person name="Mergeay M."/>
                <person name="Benotmane M.A."/>
                <person name="Vallaeys T."/>
                <person name="Michaux A."/>
                <person name="Monchy S."/>
                <person name="Dunn J."/>
                <person name="McCorkle S."/>
                <person name="Taghavi S."/>
                <person name="van der Lelie D."/>
                <person name="Richardson P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">CH34</strain>
            <plasmid evidence="11">megaplasmid</plasmid>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="12">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2010-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Benotmane R."/>
                <person name="Lapidus A."/>
                <person name="McCorkle S."/>
                <person name="Monchy S."/>
                <person name="Taghavi S."/>
                <person name="van der Lelie N."/>
                <person name="Dunn J."/>
                <person name="Leys N."/>
                <person name="Mergeay M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">CH34</strain>
            <plasmid evidence="11">megaplasmid</plasmid>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.</text>
            <dbReference type="EC" id="2.7.13.3"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.7.13.3" evidence="2"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09878.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10908.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11839.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3005"/>
    <dbReference type="EnsemblBacteria" id="ABF09878">
        <property type="protein sequence ID" value="ABF09878"/>
        <property type="gene ID" value="Rmet_3005"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF10908">
        <property type="protein sequence ID" value="ABF10908"/>
        <property type="gene ID" value="Rmet_4040"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF11839">
        <property type="protein sequence ID" value="ABF11839"/>
        <property type="gene ID" value="Rmet_4977"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3005"/>
    <dbReference type="KEGG" id="rme:Rmet_4040"/>
    <dbReference type="KEGG" id="rme:Rmet_4977"/>
    <dbReference type="eggNOG" id="COG2205">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_89_37_4"/>
    <dbReference type="OMA" id="HEVDQRD"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00082">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003660">
        <property type="entry name" value="HAMP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003661">
        <property type="entry name" value="HisK_dim/P"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036097">
        <property type="entry name" value="HisK_dim/P_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004358">
        <property type="entry name" value="Sig_transdc_His_kin-like_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00512">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00344">
        <property type="entry name" value="BCTRLSENSOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00388">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47384">
        <property type="entry name" value="SSF47384"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="SSF55874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50885">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 11" id="KW-0418">Kinase</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="11" id="KW-0614">Plasmid</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="151"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="domain" description="HAMP" evidence="8">
        <location>
            <begin position="170"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="7">
        <location>
            <begin position="230"/>
            <end position="443"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ABF09878.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ABF10908.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ABF11839.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="443" mass="48397" checksum="68C1536A3987E197" modified="2006-05-30" version="1">MNESVQLKLSFTLSLAILVVAVVAGVFSFLSAFDEAHELQDDVLRQVAQLMDRQRLLPAVPTTDIRLKDVDEESRVIVQRLGEVSPSTVGVDAGGVLPLPATLADGLHTLEVGGETFRVLVKTTASGERIAVAQESGFRNEIARDGALRTVMPFLILVPVLLLIVADLVRKMFQPIAALSKEIDQRAEQELHPVEDRHLPVEVRPFVVAINRLLARVSQSMESQRRFVADAAHELRSPLTAMSLQAERLAEAEMSSVARERLTVLRQGIERGRSLLDQLLTLAKAQSATDLPKSPVSVQSIYRRVLEDLMPLAEAKHIDIGVEGALDAEVWASELDMIAVVKNLVDNAIRYTPEGGRVDLSVGVSEGKVVLRIQDSGPGIPLAERDRVFDPFYRTLGSEQIGSGLGLSIVQTIANRIGAEIRLDFSDQEKQTGLNVAVIAPMR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="72" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LDH5</accession>
    <name>Q1LDH5_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Ubiquinone biosynthesis protein UbiV</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">ubiV</name>
        <name evidence="2" type="ordered locus">Rmet_4939</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Required for O(2)-independent ubiquinone (coenzyme Q) biosynthesis. Together with UbiU, is essential for the C6-hydroxylation reaction in the oxygen-independent ubiquinone biosynthesis pathway.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; ubiquinone biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Forms an heterodimer with UbiU.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase U32 family. UbiV subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11801.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519361.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4939"/>
    <dbReference type="MEROPS" id="U32.A01"/>
    <dbReference type="EnsemblBacteria" id="ABF11801">
        <property type="protein sequence ID" value="ABF11801"/>
        <property type="gene ID" value="Rmet_4939"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4939"/>
    <dbReference type="eggNOG" id="COG0826">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_056172_0_0_4"/>
    <dbReference type="OMA" id="CCIKYPT"/>
    <dbReference type="OrthoDB" id="1725274at2"/>
    <dbReference type="UniPathway" id="UPA00232"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008233">
        <property type="term" value="F:peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006744">
        <property type="term" value="P:ubiquinone biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02233">
        <property type="entry name" value="UbiV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001539">
        <property type="entry name" value="Peptidase_U32"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043693">
        <property type="entry name" value="UbiV"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01136">
        <property type="entry name" value="Peptidase_U32"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0408">Iron</keyword>
    <keyword evidence="1" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0831">Ubiquinone biosynthesis</keyword>
    <feature type="binding site" evidence="1">
        <location>
            <position position="49"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="183"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="196"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="200"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02233"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF11801.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="306" mass="33084" checksum="DFE4E31B939EB4BF" modified="2006-05-30" version="1">MAMPTQQSPLFRISLGPLLYYWPRATVLQFYASVADSPVDRVHLGEAVCTRRHELRHADWLEIAQMLREAGKQVVLSTPVLVESDSDIAVMRRVCGQDDYLVEANDLGAVRCMGGRPFVGGPHLNVYQGDTLAWLASLGAVGCTVPVEMDGRTMAALAAARPAGLDLEALVWGRMPLAFSARCFTARHHRLRKDACEFRCLDYPDGLVADTGEGQAFFTLNGIQTQSATCLDLCAEVSRMAEAGVGLMRVSAHSTGTFEAIAHLDGIRHGMPAAPSPVMPADAAPSNGYWTGKPGIRWIESEEATA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LDI1</accession>
    <name>Q1LDI1_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Putative response regulator receiver (CheY-like) involved in chemotaxis</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Rmet_4933</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="5">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="5">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11795.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519355.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4933"/>
    <dbReference type="EnsemblBacteria" id="ABF11795">
        <property type="protein sequence ID" value="ABF11795"/>
        <property type="gene ID" value="Rmet_4933"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4933"/>
    <dbReference type="eggNOG" id="COG0784">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG0835">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_048995_0_0_4"/>
    <dbReference type="OMA" id="RLEWNQV"/>
    <dbReference type="OrthoDB" id="1687028at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006935">
        <property type="term" value="P:chemotaxis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024181">
        <property type="entry name" value="Chemotax_regulator_CheV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036061">
        <property type="entry name" value="CheW-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002545">
        <property type="entry name" value="CheW-lke_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01584">
        <property type="entry name" value="CheW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002867">
        <property type="entry name" value="CheV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00260">
        <property type="entry name" value="CheW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50341">
        <property type="entry name" value="SSF50341"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50851">
        <property type="entry name" value="CHEW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="4" id="KW-0614">Plasmid</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CheW-like" evidence="3">
        <location>
            <begin position="19"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="domain" description="Response regulatory" evidence="2">
        <location>
            <begin position="188"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="1">
        <location>
            <position position="250"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50851"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF11795.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="320" mass="34515" checksum="04A73B989E1CCC3A" modified="2006-05-30" version="1">MTSAMRDIDERTNLTNNNQFELLLFRLGDAAHTGQSELFGINVFKVREILVMPPVTSVVGAEPSILGMADIRGQVIPVIDLPRLLECNPKSGLNIMLVTEYARSTQGFAVEAVEEIVRLDWNQVISAETSVKGGLVTSIAKLDAGGENPRLVQVLDVEQILRDVLPSRQPDVDPSTVGPALSIRPGSKILCADDSALARGLIENGLKAMGVQVVMTKSGQEAWDMLGEIADLTASKGATVRDEIALVLTDLEMPEMDGFTLTRKIKADARLKSLPVVIHSSLSGEASEEHVRKVGADAYVSKFLAKDLADTIRGVLARHP</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-10-07" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LDL7</accession>
    <name>Q1LDL7_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_4897</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11759.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4897"/>
    <dbReference type="EnsemblBacteria" id="ABF11759">
        <property type="protein sequence ID" value="ABF11759"/>
        <property type="gene ID" value="Rmet_4897"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4897"/>
    <dbReference type="HOGENOM" id="CLU_3366839_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF11759.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="35" mass="3770" checksum="7CD4AA40180C57E0" modified="2006-05-30" version="1">MLFTREAPCARDAILSALADVRRIAPSAQLTELVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="96" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LDS5</accession>
    <name>Q1LDS5_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Putative transcriptional regulator, LacI family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Rmet_4839</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="6">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="6">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11701.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519265.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4839"/>
    <dbReference type="EnsemblBacteria" id="ABF11701">
        <property type="protein sequence ID" value="ABF11701"/>
        <property type="gene ID" value="Rmet_4839"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4839"/>
    <dbReference type="eggNOG" id="COG1609">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037628_6_0_4"/>
    <dbReference type="OMA" id="HERVERY"/>
    <dbReference type="OrthoDB" id="1796334at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01392">
        <property type="entry name" value="HTH_LacI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000843">
        <property type="entry name" value="HTH_LacI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046335">
        <property type="entry name" value="LacI/GalR-like_sensor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010982">
        <property type="entry name" value="Lambda_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028082">
        <property type="entry name" value="Peripla_BP_I"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00356">
        <property type="entry name" value="LacI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13377">
        <property type="entry name" value="Peripla_BP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00354">
        <property type="entry name" value="HTH_LACI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47413">
        <property type="entry name" value="SSF47413"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53822">
        <property type="entry name" value="SSF53822"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50932">
        <property type="entry name" value="HTH_LACI_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0614">Plasmid</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH lacI-type" evidence="4">
        <location>
            <begin position="22"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABF11701.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="358" mass="38747" checksum="115B86C69BCA9B5C" modified="2006-05-30" version="1">MAPSRRTVLSQSVASAEPGHGVTLADVALAAKVSLATASRVFSHPQLVRETTANRVHEAAQRLSFRPNLLGAKLRAQQTRILGVMLPTLENAVFAECWRGIEESAMVAGYSLMLVTSGYDTERERERIEYLLRHRVDGMVLTVADAAQSLVLDQLDKESVPYVLAYNQVSLPGRHSVSVDNRASACEGVKALITAGHQRIAVISGAFMASDRARQRFAGYEDAMRAHDLTPLAPVCLPSHTASNAMQIRELMTRADAPTAFFCTNDLLAIGVISDLKRLGRRVPGDVSVLGYDGIALGALVEPPLATVMQPNAEIGAQAVHQLLQRLQHRNLNHDDAAEGAFLLPHMLRLDGTVSAPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LDS7</accession>
    <name>Q1LDS7_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_4837</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11699.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519263.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4837"/>
    <dbReference type="EnsemblBacteria" id="ABF11699">
        <property type="protein sequence ID" value="ABF11699"/>
        <property type="gene ID" value="Rmet_4837"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4837"/>
    <dbReference type="eggNOG" id="COG4102">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032896_3_1_4"/>
    <dbReference type="OMA" id="FRSMDIW"/>
    <dbReference type="OrthoDB" id="663130at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010869">
        <property type="entry name" value="DUF1501"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006311">
        <property type="entry name" value="TAT_signal"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07394">
        <property type="entry name" value="DUF1501"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51318">
        <property type="entry name" value="TAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF11699.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="430" mass="46194" checksum="4011EE792A6903C6" modified="2006-05-30" version="1">MNRRDWLRTLGGAGLALTLPTMSSRVFALPAQADARFLLVFLRGGYDAANVLVPAGSDFYYASRPTIAIKRPVADGASPDPAASLPLSADGWALHPVLGTTMLPLWQRQQLAFIPFAGTSDMSRSHFETQDGIEAGLAGDGHGGGMPRGSGFLNRLAQSMGGQAAPVAFTDGLPMVMSGALSVPNVSLKGTGRTPFDARQTQMLAQMYKGTRFESLIAEGFELRQTVAEQAEAMRKHEAAMRSAEGESMSGRMDEMQAANRRALSARGFEQEARRMAGLMRDKFNIGFIDVGGWDTHVNQGGAQGQLANLLDNLGRGIAGFAEEIGPTWRNTTVVAISEFGRTFRENGTRGTDHGHGTVYWVAGGNVRGGRIVGEQVAVTQASLNQNRDYPVLNDYRAVLGGVFRRLYGLDDARLAQVFPGTRSRDIGLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-10-07" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LE16</accession>
    <name>Q1LE16_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Thioesterase superfamily</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Rmet_4748</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="7">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="7">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the acyl coenzyme A hydrolase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11610.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4748"/>
    <dbReference type="EnsemblBacteria" id="ABF11610">
        <property type="protein sequence ID" value="ABF11610"/>
        <property type="gene ID" value="Rmet_4748"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4748"/>
    <dbReference type="eggNOG" id="COG1607">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_050164_3_0_4"/>
    <dbReference type="OMA" id="KWIDQAG"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016790">
        <property type="term" value="F:thiolester hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040170">
        <property type="entry name" value="Cytosol_ACT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033120">
        <property type="entry name" value="HOTDOG_ACOT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029069">
        <property type="entry name" value="HotDog_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006683">
        <property type="entry name" value="Thioestr_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11049">
        <property type="entry name" value="PTHR11049"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03061">
        <property type="entry name" value="4HBT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54637">
        <property type="entry name" value="SSF54637"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51770">
        <property type="entry name" value="HOTDOG_ACOT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0614">Plasmid</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HotDog ACOT-type" evidence="5">
        <location>
            <begin position="12"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="149"/>
            <end position="169"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABF11610.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="169" mass="18936" checksum="9C81A679762D87AC" modified="2006-05-30" version="1">MPESGFPAMNDLSHQLSMTVLMTPDMANFSGNVHGGTILKYLDMVAYACASRYAGRYVVTLSVDQVMFRQPIHVGELVTFLAAVNFTGRSSMEIGIKVVTENIRSKVVRHTNSCYFTMVAVDDNGAPAEVPPLQPRDEVERQRFEAAKQRRALRQEMERRHDEIKGSTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LEB6</accession>
    <name>Q1LEB6_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Fido domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Rmet_4648</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="6">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="6">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11510.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519085.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4648"/>
    <dbReference type="EnsemblBacteria" id="ABF11510">
        <property type="protein sequence ID" value="ABF11510"/>
        <property type="gene ID" value="Rmet_4648"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4648"/>
    <dbReference type="eggNOG" id="COG3177">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041789_0_0_4"/>
    <dbReference type="OMA" id="IWQQPDW"/>
    <dbReference type="OrthoDB" id="1232677at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3290.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025230">
        <property type="entry name" value="DUF4172"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003812">
        <property type="entry name" value="Fido"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036597">
        <property type="entry name" value="Fido-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040198">
        <property type="entry name" value="Fido_containing"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13504">
        <property type="entry name" value="PTHR13504"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13776">
        <property type="entry name" value="DUF4172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02661">
        <property type="entry name" value="Fic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140931">
        <property type="entry name" value="SSF140931"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51459">
        <property type="entry name" value="FIDO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0614">Plasmid</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fido" evidence="4">
        <location>
            <begin position="119"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="365"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="211"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="215"/>
            <end position="222"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="253"/>
            <end position="254"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRSR" id="PIRSR640198-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR640198-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51459"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABF11510.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="384" mass="42441" checksum="0F50385315CC9736" modified="2006-05-30" version="1">MNRGEYTYIWQAPDWPNWRYDLGALASQMAEVSRAQGMLMGRLADLGMGLRDQACLAALTDDVVKTSEIEGEHLSVHSVRSSIARRLGVEIGALAPVDRHVDGVVEMVLDATTNSQAPVTQARLFGWHAALFPTGYSGLSRINVAHWRDDSAGPMQVVSGPIGRQRVHFEAPPAERLPAETDRFLAWINAPSGEAPLIRAGLAHLWFVTLHPFDDGNGRIARAIGDLMLARADGSTQRFYSLSAQIQRERNAYYDILERTQKDAMDVTPWLSWFLETLHRAVDQAQVTLDDVLAKSRFWQHWGTQALNARQIKVLNRLLDGFDGKLTSSKWAAIAKCSSDTALRDLNDLVERGMLRKAAAGGRSTSYELNDVPGAPATRGDGNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="100" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LEI3</accession>
    <name>Q1LEI3_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Transcriptional regulator, TetR family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Rmet_4581</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="5">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="5">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11443.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4581"/>
    <dbReference type="EnsemblBacteria" id="ABF11443">
        <property type="protein sequence ID" value="ABF11443"/>
        <property type="gene ID" value="Rmet_4581"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4581"/>
    <dbReference type="eggNOG" id="COG1309">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_053314_0_0_4"/>
    <dbReference type="OMA" id="CFLHTIA"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001647">
        <property type="entry name" value="HTH_TetR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036271">
        <property type="entry name" value="Tet_transcr_reg_TetR-rel_C_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00440">
        <property type="entry name" value="TetR_N"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00455">
        <property type="entry name" value="HTHTETR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48498">
        <property type="entry name" value="SSF48498"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50977">
        <property type="entry name" value="HTH_TETR_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0614">Plasmid</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH tetR-type" evidence="3">
        <location>
            <begin position="58"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="domain" description="HTH tetR-type" evidence="3">
        <location>
            <begin position="268"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="H-T-H motif" evidence="1">
        <location>
            <begin position="81"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="H-T-H motif" evidence="1">
        <location>
            <begin position="291"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="15"/>
            <end position="29"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF11443.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="453" mass="50228" checksum="FC1E77750466D0AB" modified="2006-05-30" version="1">MFDNRKVCADPRPARNRSSPTSHSHIIRSTQPAGPRPLRAQPLMLTDQSEARQTRRYVEKREVILDTAAALFNRKGVRGTTLADVGQQVGLSTNSVTYYYRKKEDLVLACLMRAIAETSGLVDRAAQADSPSERARAFITLFVARLARTAVGEKPELMSLREIRALPESHVEVAFAAFNDMFRRVRGLLCDALPSDPTQRSALGARAHLLWSLTSGAAGWIDRYETDDYPKIAQTLADIVLNGIAGPKSAWQAECDVTALLPALAQPETTQQAFLRAATELLNEQGYRGASADRISARLNLTKGAFYHHNENKDDLISACFDRMSTVIRNTQTLVDDLPGTGWDKLCAVSRALVHYQFSSQGPLLRLTIYGALPEEMRIAKMRDMARLSTRFVRLLVQGMQDGSVRPLDQAIAALQVHGMINAAVELRRWVRDADADNAADLFVRPMMVGLLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="52" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LEM0</accession>
    <name>Q1LEM0_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_4541</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11406.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518997.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF11406">
        <property type="protein sequence ID" value="ABF11406"/>
        <property type="gene ID" value="Rmet_4541"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4541"/>
    <dbReference type="HOGENOM" id="CLU_1303207_0_0_4"/>
    <dbReference type="OMA" id="CFLTICT"/>
    <dbReference type="OrthoDB" id="1569238at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF11406.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="185" mass="19518" checksum="3196B5DB741C4185" modified="2006-05-30" version="1">MNLAAILVSASVAASVAVSSTPVERQLLAISPHDFAQQFNAVAHEAGAKMVLPDFHAKPGWHRAKTSEGISVMVRGAPTGYAIDEVIVVCQTGEQCLQTISTAAQAIDSTIDQTLLQYFVAARIAEQIGEVALVISGLVYLVVSPDDQDYVALVIRPYLPQQELTAKTQAALQPAALPPPGALTM</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="119" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LF12</accession>
    <name>Q1LF12_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Spermidine/putrescine import ATP-binding protein PotA</fullName>
            <ecNumber evidence="9">7.6.2.11</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">potG</name>
        <name evidence="9" type="synonym">potA</name>
        <name evidence="11" type="ordered locus">Rmet_4399</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="12">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="12">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Part of the ABC transporter complex PotABCD involved in spermidine/putrescine import. Responsible for energy coupling to the transport system.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">The complex is composed of two ATP-binding proteins (PotA), two transmembrane proteins (PotB and PotC) and a solute-binding protein (PotD).</text>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the ABC transporter superfamily. Spermidine/putrescine importer (TC 3.A.1.11.1) family.</text>
    </comment>
    <dbReference type="EC" id="7.6.2.11" evidence="9"/>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11264.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518881.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4399"/>
    <dbReference type="EnsemblBacteria" id="ABF11264">
        <property type="protein sequence ID" value="ABF11264"/>
        <property type="gene ID" value="Rmet_4399"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4399"/>
    <dbReference type="eggNOG" id="COG3842">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_1_4"/>
    <dbReference type="OMA" id="PFSIRPE"/>
    <dbReference type="OrthoDB" id="1200451at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043190">
        <property type="term" value="C:ATP-binding cassette (ABC) transporter complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015417">
        <property type="term" value="F:ABC-type polyamine transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008995">
        <property type="entry name" value="Mo/tungstate-bd_C_term_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005893">
        <property type="entry name" value="Sp_pt_ABC_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013611">
        <property type="entry name" value="Transp-assoc_OB_typ2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08402">
        <property type="entry name" value="TOBE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50331">
        <property type="entry name" value="SSF50331"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01187">
        <property type="entry name" value="potA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="2 9" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 8 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-0614">Plasmid</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9" id="KW-1278">Translocase</keyword>
    <keyword evidence="1 9" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ABC transporter" evidence="10">
        <location>
            <begin position="4"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="36"/>
            <end position="43"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU364083"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ABF11264.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="364" mass="39316" checksum="5A8B80397F2C685A" modified="2006-05-30" version="1">MDLLQVHHLSKSFGGRPVLDDIGFNVGSGQFFTLVGPSGCGKTTLLRLLGGFSEPDSGEVLFDGKSLQGVPPEHRPMHTVFQSYALFPHMTVAANIAFPLRMQKLPRHEIDGRLGSLIENVRLQGFERYYPHELSGGQRQRVAIARALAARPRLLLLDEPLSALDAALQTQMLHELIALQQQLGVAFIYVTHDQTHALALSQRIAVMHEGHIVQLDTPEAIYRAPATTFVAGFIGSCNLLDATVAESSAARLRVRIAGVGERDMPAGQALAIGSRGTVAIRPEEIQLRPRGASPGGNASAPMLEGAVVDRLYQGDVTLYTVELASGHRLQTMVANAGPVTDGQFTRGDRVELDWPGNAGVFLSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="97" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LFY8</accession>
    <name>Q1LFY8_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Transcriptional regulator, LysR family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_4070</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the LysR transcriptional regulatory family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10938.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008644346.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4070"/>
    <dbReference type="EnsemblBacteria" id="ABF10938">
        <property type="protein sequence ID" value="ABF10938"/>
        <property type="gene ID" value="Rmet_4070"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4070"/>
    <dbReference type="eggNOG" id="COG0583">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039613_6_4_4"/>
    <dbReference type="OMA" id="ERWICQP"/>
    <dbReference type="OrthoDB" id="1439189at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005119">
        <property type="entry name" value="LysR_subst-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000847">
        <property type="entry name" value="Tscrpt_reg_HTH_LysR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00126">
        <property type="entry name" value="HTH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03466">
        <property type="entry name" value="LysR_substrate"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00039">
        <property type="entry name" value="HTHLYSR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50931">
        <property type="entry name" value="HTH_LYSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH lysR-type" evidence="2">
        <location>
            <begin position="1"/>
            <end position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF10938.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="297" mass="31974" checksum="0A5CFCFEA3B142D2" modified="2006-05-30" version="1">MDLRQLQQFVVLAETGNFHRAAERLHMAQPPLSISIRKLEESLGTPLFVRTTRGVRLTQAGEAALNDARRALFHAGQARAAAVSAALGERGALRIGFVGSATYALLPRLIPAFRSAHPGIDLILHESTSAAILDRIEHGQLDAGLVRFPILNSGPYTLTPLESDAFVAAVPADGRFAEQESIALKALADEPFIMHPRADVPNLQAVAMLLCQQAGFVPRIAQEAVQVQTIVSLVESGLGVALVPGVAARYTNRRVRFLRLSSPRPAARIGIALATQQDTDDRHVQRFIAAAQRIAGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LG12</accession>
    <name>Q1LG12_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Gamma-glutamyltranspeptidase</fullName>
            <ecNumber evidence="1">2.3.2.2</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">ggt</name>
        <name evidence="1" type="ordered locus">Rmet_4046</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EC" id="2.3.2.2" evidence="1"/>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10914.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4046"/>
    <dbReference type="EnsemblBacteria" id="ABF10914">
        <property type="protein sequence ID" value="ABF10914"/>
        <property type="gene ID" value="Rmet_4046"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4046"/>
    <dbReference type="eggNOG" id="COG0405">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014813_3_2_4"/>
    <dbReference type="OMA" id="FDGWMLM"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102953">
        <property type="term" value="F:hypoglycin A gamma-glutamyl transpeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0103068">
        <property type="term" value="F:leukotriene C4 gamma-glutamyl transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.246.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043138">
        <property type="entry name" value="GGT_lsub_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043137">
        <property type="entry name" value="GGT_ssub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="SSF56235"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF10914.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="624" mass="67950" checksum="26C388505D425F2C" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LGF6</accession>
    <name>Q1LGF6_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative short-chain dehydrogenase</fullName>
            <ecNumber evidence="2">1.1.1.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_3902</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.-" evidence="2"/>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10770.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3902"/>
    <dbReference type="EnsemblBacteria" id="ABF10770">
        <property type="protein sequence ID" value="ABF10770"/>
        <property type="gene ID" value="Rmet_3902"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3902"/>
    <dbReference type="eggNOG" id="COG1028">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010194_1_3_4"/>
    <dbReference type="OMA" id="EWGPQFG"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00061">
        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU000363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF10770.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="350" mass="37118" checksum="1C246C7E569ACC41" modified="2006-05-30" version="1">MASAGTKPTFVIHEFQSFGRQFIVSSSAIKRAGFPVRADSKPMEDTMSGMMEGKVVVVTGAGGGIGRGIALGMAAAGARVVVNDLGVSMSGEGGDAGPAQRVVEEIRAAGGQAVANTDSVSTWAGGNAIVQCALDTFGRIDAVVNNAGNLRDRMFFKMNEEEWRSVIDVHLHGTFFVSRAAANYFKDQESGAYVNMTSTSGLIGNLGQANYSAAKLGIAALSKSIALDMARFNVRSNCIAPFAWSRMTSSIPAETPAEKERVARLQKMEAGKIAPVAVFLASDAAKDVNAQIFAVRANEIMLMSQSRPVRSVHTGEGWTPETVAEIAMPAMRGSFFKLDRSPDVINWDPI</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="66" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LGK8</accession>
    <name>Q1LGK8_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative thiol-disulphide oxidoreductase DCC</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_3850</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10718.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3850"/>
    <dbReference type="EnsemblBacteria" id="ABF10718">
        <property type="protein sequence ID" value="ABF10718"/>
        <property type="gene ID" value="Rmet_3850"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3850"/>
    <dbReference type="eggNOG" id="COG3011">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_978864_0_0_4"/>
    <dbReference type="OMA" id="HRIVEQS"/>
    <dbReference type="OrthoDB" id="1144865at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015035">
        <property type="term" value="F:protein-disulfide reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007263">
        <property type="entry name" value="DCC1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044691">
        <property type="entry name" value="DCC1_Trx"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34290">
        <property type="entry name" value="PTHR34290"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04134">
        <property type="entry name" value="DCC1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="66"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="161"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="210"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="242"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="266"/>
            <end position="288"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF10718.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="293" mass="32514" checksum="3F20E89AB7E9091B" modified="2006-05-30" version="1">MNALVLYFDGKCPFCAAEMRRLSEWDTQHQLAFVDIAQEGFDCSSLNVDLAALNRELHGRTSDGRLLVGIDCMIAAYTLAGHGWLVLPLRVPGLRRVLANLYRAFAGNRYTLSRWLGYGTATACDGDSCGTGNPFLSGKELTDAAEDRPARRDSRQLAVRWMYAAAIAHLLVGAAMPWAADATLFGTYHRIVEQSFWSGAAPAQARAQQVWWIALFGATLQCSAIWMLALVHLGNRTRHRMAWGWLLVGLLVWAPQDLLISLQAHVWPHVLVDAVALLTMIPPLLYLWRKDAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="58" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LGV2</accession>
    <name>Q1LGV2_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_3752</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10624.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518271.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3752"/>
    <dbReference type="EnsemblBacteria" id="ABF10624">
        <property type="protein sequence ID" value="ABF10624"/>
        <property type="gene ID" value="Rmet_3752"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3752"/>
    <dbReference type="eggNOG" id="ENOG50316GC">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_173088_0_0_4"/>
    <dbReference type="OMA" id="ASIVICR"/>
    <dbReference type="OrthoDB" id="1790922at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF10624.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="100" mass="11101" checksum="9794587FB5299D70" modified="2006-05-30" version="1">MSIASISNAPPFAFYKGFDLYPLVYKNQPVQTWPRVKPDGRFNASIVICRAGYRPGSEHTRVFRLENALWENIGTARRGAVQFGEDIINGLVAGESVVSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LH63</accession>
    <name>Q1LH63_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_3641</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10513.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008646871.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF10513">
        <property type="protein sequence ID" value="ABF10513"/>
        <property type="gene ID" value="Rmet_3641"/>
    </dbReference>
    <dbReference type="GeneID" id="60826197"/>
    <dbReference type="KEGG" id="rme:Rmet_3641"/>
    <dbReference type="HOGENOM" id="CLU_136209_4_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193122" evidence="1">
        <location>
            <begin position="21"/>
            <end position="90"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF10513.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="90" mass="9570" checksum="6E9165A185AF903A" modified="2006-05-30" version="1">MKTLALSLLCAIGLSVGATAAQASEFNSDLARLDGSIARKVDPYLDGARGVTEKRDVYTEGARGVTEKRDVYTEGARGVTEKRDVYSEGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LHC3</accession>
    <name>Q1LHC3_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Putative multidrug RND transporter, membrane fusion protein (AcrA-MtdA-HylD family)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Rmet_3581</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10453.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517995.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3581"/>
    <dbReference type="EnsemblBacteria" id="ABF10453">
        <property type="protein sequence ID" value="ABF10453"/>
        <property type="gene ID" value="Rmet_3581"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3581"/>
    <dbReference type="eggNOG" id="COG0845">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018816_2_0_4"/>
    <dbReference type="OMA" id="QNDFGKA"/>
    <dbReference type="OrthoDB" id="1432684at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032317">
        <property type="entry name" value="HlyD_D23"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006143">
        <property type="entry name" value="RND_pump_MFP"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16576">
        <property type="entry name" value="HlyD_D23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01730">
        <property type="entry name" value="RND_mfp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="31"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="domain" description="HlyD_D23" evidence="3">
        <location>
            <begin position="89"/>
            <end position="312"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF16576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF10453.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="407" mass="43031" checksum="7A21AB4921D9D29A" modified="2006-05-30" version="1">MKTDGIDQPKGFVDGNWSAAAGTGRGRTSRWPAVVAVLVIVVAGWFGWKHWFAPKPAAKGPAVTTVATAIVQQGDVPLEVNANGNVVAMQTVEVRPQVSSTVRTVHIKEGQSVKPGDLLFSLDTRMDEANLDKARAQLMRDQADLSDARRTLTRSQELLQRNFISRSAVDTAQAKVDGFEATIRADQAAIEASRVAISYGTIRATIGGRTGVINVFPGSLVTPTTATPMVIVAQIAPITVMFTLPERQLAALREALRSGPVSVTALPNDGSKQPVTGKITFVDNTVDPQYGSIRVKAMFGNEEHRLWPGTYANVNATVQVLKDALSVPPQAIVAGPEGRFVYTVQADNKVARVPVQVIATTAAAAVIEGVQPGARVVTEGAQNLRPGSLVREAAARGASAPAAATKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LHR1</accession>
    <name>Q1LHR1_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">N-carbamoyl-L-amino-acid amidohydrolase</fullName>
            <ecNumber evidence="4">3.5.1.87</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Rmet_3443</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="2">Binds 2 Zn(2+) ions per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase M20 family.</text>
    </comment>
    <dbReference type="EC" id="3.5.1.87" evidence="4"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10315.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517875.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3443"/>
    <dbReference type="EnsemblBacteria" id="ABF10315">
        <property type="protein sequence ID" value="ABF10315"/>
        <property type="gene ID" value="Rmet_3443"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3443"/>
    <dbReference type="eggNOG" id="COG0624">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024588_6_1_4"/>
    <dbReference type="OMA" id="AFIEVHI"/>
    <dbReference type="OrthoDB" id="829830at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016813">
        <property type="term" value="F:hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amidines"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050538">
        <property type="term" value="F:N-carbamoyl-L-amino-acid hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010158">
        <property type="entry name" value="Amidase_Cbmase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036264">
        <property type="entry name" value="Bact_exopeptidase_dim_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002933">
        <property type="entry name" value="Peptidase_M20"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32494">
        <property type="entry name" value="PTHR32494"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01546">
        <property type="entry name" value="Peptidase_M20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001235">
        <property type="entry name" value="Amidase_carbamoylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55031">
        <property type="entry name" value="SSF55031"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01879">
        <property type="entry name" value="hydantase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0862">Zinc</keyword>
    <feature type="binding site" evidence="2">
        <location>
            <position position="87"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="98"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="133"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="197"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="222"/>
        </location>
        <ligand>
            <name>allantoate</name>
            <dbReference type="ChEBI" id="CHEBI:17536"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="281"/>
        </location>
        <ligand>
            <name>allantoate</name>
            <dbReference type="ChEBI" id="CHEBI:17536"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="294"/>
        </location>
        <ligand>
            <name>allantoate</name>
            <dbReference type="ChEBI" id="CHEBI:17536"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="389"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006153"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR001235-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR001235-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF10315.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="418" mass="44415" checksum="FEC172D07BBEB6D0" modified="2006-05-30" version="1">MAATPTPLSAETGTRIMTWADALAVHTDQPGILTRTYLTEAHHGAAALLTEWMEAAGMTVRRDAAGNVIGRYEGTTPNAPALLTGSHFDTVRDAGRYDGNLGVILPIACVAEWNRQGKRFPFALEVVGFAEEEGVRFKATLLGSRAIAGTFDTNVLDNVDDSGKTMREVMRAAGFDAGQLPAAKHDRSQVLAFIEVHIEQGPVLLNEGLPVGVVTAISGATRFIVELEGLAGHAGTVPMDMRRDAAMAGAEIGLFIEKRCGGKPGLVGTVGQFNVPNGATNVVPGRAVFSIDIRAGDDAEREAAVNDVLAEIERVCARRNVRSQIRKTHEAASVPCAPWLQSQWAAAVERQGVPVHHLPSGAGHDAMAIAAIADVAMLFVRCGNGGISHHPTETMTSEDAQTAARVFADFVEHFHRPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-12-02" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LHS1</accession>
    <name>Q1LHS1_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative metal-binding hydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">ycbL</name>
        <name evidence="2" type="ordered locus">Rmet_3433</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10305.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3433"/>
    <dbReference type="EnsemblBacteria" id="ABF10305">
        <property type="protein sequence ID" value="ABF10305"/>
        <property type="gene ID" value="Rmet_3433"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3433"/>
    <dbReference type="eggNOG" id="COG0491">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030571_5_0_4"/>
    <dbReference type="OMA" id="GAWGTNC"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001279">
        <property type="entry name" value="Metallo-B-lactamas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00753">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00849">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="SSF56281"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Lactamase_B" evidence="1">
        <location>
            <begin position="18"/>
            <end position="198"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF10305.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="222" mass="24651" checksum="6941E6965C19F7A6" modified="2006-05-30" version="1">MPQQAQMKALLIPVTPFQQNCSLLIDETTQRAAVCDPGGDLDRILSAVKEQGVTLEKIFLTHGHVDHCAGAATLSRQLGIPIEGPQQEERFWIEQLPEQTRRFGFGHPEAFEPDRWLENGDTVTFGNETLEVYHCPGHTPGHVVFFSRPNKLAIVGDVLFAGSIGRTDFPRGNHADLIRSIRTRLWPLGDDVTFVPGHGPVSTFGEERRNNPFVADHLIDVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="69" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LHV5</accession>
    <name>Q1LHV5_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative Permease of the major facilitator superfamily MFS_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_3399</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10271.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517834.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3399"/>
    <dbReference type="EnsemblBacteria" id="ABF10271">
        <property type="protein sequence ID" value="ABF10271"/>
        <property type="gene ID" value="Rmet_3399"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3399"/>
    <dbReference type="eggNOG" id="COG2814">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001265_59_0_4"/>
    <dbReference type="OMA" id="MRLSLYD"/>
    <dbReference type="OrthoDB" id="1594866at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="SSF103473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="55"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="87"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="110"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="146"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="231"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="296"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="352"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="382"/>
            <end position="404"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF10271.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="417" mass="44668" checksum="1C60030F16A41855" modified="2006-05-30" version="1">MNAPSVPHPAGGPALPPRQIVWTLGITEIISWGTLYFAFTVFIEPMRQSLGYGKPFLAGGFSLGLLVWAMCSFAVGRLLDRVPARKVMGTGSVLAAIGLLLWAWCPSPAVFLLMWIPLGLAMASTLYEPAFVVLRQAWGDGYQKPIVHVTLLAGFASTIFIPLAQWLVLHLGWRETLVVFAALNLFVCAPLHARLRYAVHPSYAHAATHSGTPLAGAASSGIARATLRQPVFWCVVLAFTATAVIASLLGAHLIPMLTEKGMPVAQQLIVAALIGPAQVAGRLAMARLPIDHPVRLSMPVYIVMTIGLACLALGNGYWLMFAAICYGCANGVNTMLRAMAMPELISRHHYATLNGLMMTPVLLMQAAAPWFGALLWRAAGGYWLMLWVMVALSIGALAAFAFALHQRGLLRAGAAPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-10-07" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LHZ2</accession>
    <name>Q1LHZ2_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_3362</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10234.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3362"/>
    <dbReference type="EnsemblBacteria" id="ABF10234">
        <property type="protein sequence ID" value="ABF10234"/>
        <property type="gene ID" value="Rmet_3362"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3362"/>
    <dbReference type="HOGENOM" id="CLU_2466759_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF10234.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="88" mass="10014" checksum="B411A3B7A700F647" modified="2006-05-30" version="1">MRRQYGLSFPKLTRVRLQHASVVTRQSEHLTTPPRVPKLQTGLISPGILPMLLQFEVVLALWAALAYSVCERPYSTCHGRQSPCQLHF</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LIE0</accession>
    <name>Q1LIE0_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Extra-cytoplasmic Solute Receptor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">bug</name>
        <name evidence="4" type="ordered locus">Rmet_3214</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Periplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the UPF0065 (bug) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10086.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517696.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3214"/>
    <dbReference type="EnsemblBacteria" id="ABF10086">
        <property type="protein sequence ID" value="ABF10086"/>
        <property type="gene ID" value="Rmet_3214"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3214"/>
    <dbReference type="eggNOG" id="COG3181">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045683_0_0_4"/>
    <dbReference type="OMA" id="GGSTDQF"/>
    <dbReference type="OrthoDB" id="447734at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042597">
        <property type="term" value="C:periplasmic space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005064">
        <property type="entry name" value="BUG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042100">
        <property type="entry name" value="Bug_dom1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42928">
        <property type="entry name" value="PTHR42928"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03401">
        <property type="entry name" value="TctC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017082">
        <property type="entry name" value="YflP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0675">Receptor</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193579" evidence="3">
        <location>
            <begin position="25"/>
            <end position="323"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF10086.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="323" mass="34312" checksum="620C87B2A2005C44" modified="2006-05-30" version="1">MHRRTAILAAAALGLATTLSSASAAWPDRPIKLVVPYAPGGSTDQFGRGIAEGVSRELKQPVLVENRPGANTMIGTQSVARAQPDGYTVLLVTSASMVLNPMLYKKPGYEPQEFKIITVGVEAPLVVVVNNQVPSNNIKEFAAYAKAANGKLNYSSVGLGNSLQLATEMLKTELGIEMTHVPYNGSAPALSALLGNNVQLMVDVVSTSLPHIKAGKLKALAVTGSSRLDVLPNVPTVAESGYPNFRAATWFGLAVPAHTPPEAVAKLQAAASHVLTEAQFRNTFNALGFVVQPPRTQAEIDRYVTADRDNWGKVIRANHITLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LIM0</accession>
    <name>Q1LIM0_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Peptidoglycan D,D-transpeptidase FtsI</fullName>
            <ecNumber evidence="6">3.4.16.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Penicillin-binding protein 3</fullName>
            <shortName evidence="6">PBP-3</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6 10" type="primary">ftsI</name>
        <name evidence="10" type="ordered locus">Rmet_3134</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Catalyzes cross-linking of the peptidoglycan cell wall at the division septum.</text>
    </comment>
    <comment type="pathway">
        <text evidence="6">Cell wall biogenesis; peptidoglycan biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the transpeptidase family. FtsI subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.4.16.4" evidence="6"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10006.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517624.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3134"/>
    <dbReference type="EnsemblBacteria" id="ABF10006">
        <property type="protein sequence ID" value="ABF10006"/>
        <property type="gene ID" value="Rmet_3134"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3134"/>
    <dbReference type="eggNOG" id="COG0768">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009289_6_2_4"/>
    <dbReference type="OMA" id="HGKEEYY"/>
    <dbReference type="OrthoDB" id="213680at2"/>
    <dbReference type="UniPathway" id="UPA00219"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005887">
        <property type="term" value="C:integral component of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008658">
        <property type="term" value="F:penicillin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008955">
        <property type="term" value="F:peptidoglycan glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009002">
        <property type="term" value="F:serine-type D-Ala-D-Ala carboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000917">
        <property type="term" value="P:division septum assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043093">
        <property type="term" value="P:FtsZ-dependent cytokinesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009252">
        <property type="term" value="P:peptidoglycan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008360">
        <property type="term" value="P:regulation of cell shape"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.710.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02080">
        <property type="entry name" value="FtsI_transpept"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012338">
        <property type="entry name" value="Beta-lactam/transpept-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037532">
        <property type="entry name" value="FtsI_transpept"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005311">
        <property type="entry name" value="PBP_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036138">
        <property type="entry name" value="PBP_dimer_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001460">
        <property type="entry name" value="PCN-bd_Tpept"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03717">
        <property type="entry name" value="PBP_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00905">
        <property type="entry name" value="Transpeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56519">
        <property type="entry name" value="SSF56519"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56601">
        <property type="entry name" value="SSF56601"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0121">Carboxypeptidase</keyword>
    <keyword evidence="5 6" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="1 6" id="KW-0132">Cell division</keyword>
    <keyword evidence="6" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0133">Cell shape</keyword>
    <keyword evidence="6" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="2 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0573">Peptidoglycan synthesis</keyword>
    <keyword evidence="6" id="KW-0645">Protease</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0717">Septation</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="PBP_dimer" evidence="9">
        <location>
            <begin position="87"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="domain" description="Transpeptidase" evidence="8">
        <location>
            <begin position="277"/>
            <end position="570"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="active site" description="Acyl-ester intermediate" evidence="6">
        <location>
            <position position="323"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00905"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF03717"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ABF10006.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="605" mass="66116" checksum="6A3F6147B615AE3E" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="64" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LIS4</accession>
    <name>Q1LIS4_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Putative 4-oxalocrotonate tautomerase protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_3080</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09952.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008645891.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3080"/>
    <dbReference type="EnsemblBacteria" id="ABF09952">
        <property type="protein sequence ID" value="ABF09952"/>
        <property type="gene ID" value="Rmet_3080"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3080"/>
    <dbReference type="eggNOG" id="COG1942">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_154654_1_0_4"/>
    <dbReference type="OMA" id="TQEYRHQ"/>
    <dbReference type="OrthoDB" id="1979126at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.429.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014347">
        <property type="entry name" value="Tautomerase/MIF_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55331">
        <property type="entry name" value="SSF55331"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF09952.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="132" mass="14482" checksum="1A17484FD0E97394" modified="2006-05-30" version="1">MPHIALQISGHPDTELTRRAVAAVGELTQRVLRKEASVIATTVTYIPREQWFIAGKPLSEYAEGRNAFQLDISVTDETNTKAEKAQYLAAIFGALSEVIGDVNPLSYIHVIDARAAAYGYGGKSQEFRHQHG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2007-02-06" modified="2022-08-03" version="101" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LJ08</accession>
    <name>PHNC2_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Phosphonates import ATP-binding protein PhnC 2</fullName>
            <ecNumber evidence="1">7.3.2.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">phnC2</name>
        <name type="ordered locus">Rmet_2995</name>
    </gene>
    <organism>
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Part of the ABC transporter complex PhnCDE involved in phosphonates import. Responsible for energy coupling to the transport system.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + H2O + phosphonate(out) = ADP + H(+) + phosphate + phosphonate(in)</text>
            <dbReference type="Rhea" id="RHEA:18065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16215"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="7.3.2.2"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">The complex is composed of two ATP-binding proteins (PhnC), two transmembrane proteins (PhnE) and a solute-binding protein (PhnD).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell inner membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the ABC transporter superfamily. Phosphonates importer (TC 3.A.1.9.1) family.</text>
    </comment>
    <dbReference type="EC" id="7.3.2.2" evidence="1"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09868.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_003056095.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q1LJ08"/>
    <dbReference type="SMR" id="Q1LJ08"/>
    <dbReference type="STRING" id="266264.Rmet_2995"/>
    <dbReference type="EnsemblBacteria" id="ABF09868">
        <property type="protein sequence ID" value="ABF09868"/>
        <property type="gene ID" value="Rmet_2995"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2995"/>
    <dbReference type="eggNOG" id="COG3638">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_22_4"/>
    <dbReference type="OMA" id="ALINCPK"/>
    <dbReference type="OrthoDB" id="1181903at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015416">
        <property type="term" value="F:ABC-type phosphonate transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03256">
        <property type="entry name" value="ABC_PhnC_transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012693">
        <property type="entry name" value="ABC_transpr_PhnC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02315">
        <property type="entry name" value="ABC_phnC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51249">
        <property type="entry name" value="PHNC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0997">Cell inner membrane</keyword>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0918">Phosphonate transport</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-1278">Translocase</keyword>
    <keyword id="KW-0813">Transport</keyword>
    <feature type="chain" id="PRO_0000274736" description="Phosphonates import ATP-binding protein PhnC 2">
        <location>
            <begin position="1"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="1">
        <location>
            <begin position="10"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="253"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="43"/>
            <end position="50"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01713"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="279" mass="30166" checksum="78F7F6F7FD03ADED" modified="2006-05-30" version="1">MKLEKDRDVLSLKGVSVRYVDSTVALHPTSLDVKQGEFLVLLGASGAGKSTLLRSINGLVLPTKGEVSIPGLAGGVVNAKTLREHRKRCGMVFQQHHLIGRQSVLRNVLMGKLGDRGAFASLWPWSKKDKLEALTVIERVGLLEKALSRADALSGGQQQRVGIARALIQKPRILLADEPVASLDPATAHSVLTLLHEICKKDHLTAIVSLHQVELARSFADRIIGLRQGAVVFEGRAEQLSPDVARNLYAKQSNASNTSASTDSPRTLQSSQTKELLPC</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LJ76</accession>
    <name>Q1LJ76_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Ion transport protein, VIC superfamily</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">kch</name>
        <name evidence="13" type="ordered locus">Rmet_2927</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09800.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517472.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2927"/>
    <dbReference type="EnsemblBacteria" id="ABF09800">
        <property type="protein sequence ID" value="ABF09800"/>
        <property type="gene ID" value="Rmet_2927"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2927"/>
    <dbReference type="eggNOG" id="COG2126">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011722_1_3_4"/>
    <dbReference type="OMA" id="KGIYWAI"/>
    <dbReference type="OrthoDB" id="1991946at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008076">
        <property type="term" value="C:voltage-gated potassium channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005249">
        <property type="term" value="F:voltage-gated potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.350">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028325">
        <property type="entry name" value="VG_K_chnl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027359">
        <property type="entry name" value="Volt_channel_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11537">
        <property type="entry name" value="PTHR11537"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="10" id="KW-0407">Ion channel</keyword>
    <keyword evidence="8" id="KW-0406">Ion transport</keyword>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0630">Potassium</keyword>
    <keyword evidence="5" id="KW-0631">Potassium channel</keyword>
    <keyword evidence="3" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="45"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="72"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="102"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="144"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="168"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="231"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="domain" description="Ion_trans" evidence="12">
        <location>
            <begin position="42"/>
            <end position="249"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ABF09800.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="307" mass="34761" checksum="B9E95597D1D19091" modified="2006-05-30" version="1">MSRSTRSDQNARIRQRLGQPESGWRQRWYTIIFEADTRPGRTFDIVLLVAIIASVAVVMLDSIQAIHARLGTVFTALEWLFTLLFTTEYIMRLSVVRRPLRYVFSFYGIIDFLSIIPTWASFFVPELAFLIDVRLLRLLRVFRILKLTVYFEEAEILMLALSNSRRKIFVFLGTVFIITVILGTIMYVVEGPTHGFTSIPVSMYWAIVTLTTTGFGDMVPKTPIGQFITSITILLGYGIIAFPTGIVGAELAASIIRRPLTTRSCEHCLTEGHEPDATFCKHCGTALPEYRTELATDRRGDDGSRSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LJS4</accession>
    <name>Q1LJS4_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Glycosyl transferase, family 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_2729</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09602.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2729"/>
    <dbReference type="CAZy" id="GT2">
        <property type="family name" value="Glycosyltransferase Family 2"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF09602">
        <property type="protein sequence ID" value="ABF09602"/>
        <property type="gene ID" value="Rmet_2729"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2729"/>
    <dbReference type="eggNOG" id="COG1216">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023845_0_5_4"/>
    <dbReference type="OMA" id="AHIRMFA"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="SSF53448"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF09602.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="298" mass="33052" checksum="957AE87FA5C72688" modified="2006-05-30" version="1">MLDAMMSLRQFAANSIASVAIVDNASVDDSLDLVAERDDWGFPVHLIRNAENRGFGAACNQGAGVGRAEFVLLLNPDTRVYENSLSIPLAFMQRSENAAVSVTGIQLIDETGHVQRACARFPSPAMFSAAAFGLDRLPWLRGQRLHMSDWDHASTRDVDHVIGAFYLIRRSVFERLGGFDERFFVYLEDLDLSLRVKQAGGRIVFLADAQAFHAGGGTSRQIKATRLFYSLRSRLLYGFKHFSRVAAWALLVVTILPEPITRTVFSAARGGMQDVGNTLRGYGMLLRSLPQILRASRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LKC7</accession>
    <name>Q1LKC7_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Major facilitator superfamily MFS_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Rmet_2522</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09399.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517098.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2522"/>
    <dbReference type="EnsemblBacteria" id="ABF09399">
        <property type="protein sequence ID" value="ABF09399"/>
        <property type="gene ID" value="Rmet_2522"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2522"/>
    <dbReference type="eggNOG" id="COG2814">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001265_59_9_4"/>
    <dbReference type="OMA" id="ALNWPVR"/>
    <dbReference type="OrthoDB" id="1594866at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="SSF103473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="48"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="75"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="101"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="135"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="165"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="217"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="250"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="280"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="304"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="336"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="370"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="domain" description="MFS" evidence="6">
        <location>
            <begin position="8"/>
            <end position="395"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABF09399.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="403" mass="43101" checksum="0750D07E9E39D0AF" modified="2006-05-30" version="1">MTDRKLSLTTVLVCGGLLVTLSMGIRHGFGLFNLPITQTHGWSRETFAFALALQNLMWGASQPITGALADKFGALRIMIIGVALYVAGLIVMGLATSGTAFATGAGVLIGIAQSGTTYSVVYGVIGRVASAEKRVWAMGIAAAAGSFGQFLMIPVEQTLISTMGWQNALFILAFLACFMLPLALTLREPKMVATTTGHHQTIGEATREAFGNRNFQLLTLGYFVCGFQVVFIGVHLAPYLKDRGFTDPKVATVALALIGLFNVFGTYTAGSMGQRMPKRYLLSFIYIARSVVIAGYLLMPLTAASTWVFAALMGFLWLSTVPLTNGIIAQVFGVKYLSMLSGVVFFSHQIGSFLGAWLGGYLFDKTGSYSTVWMIAIALGLMAAVVNLPIREQALVRPQPVGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LKI3</accession>
    <name>Q1LKI3_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Methyltransferase</fullName>
            <ecNumber evidence="4">2.1.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Rmet_2466</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the N(4)/N(6)-methyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.-" evidence="4"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09343.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517056.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2466"/>
    <dbReference type="REBASE" id="12354">
        <property type="entry name" value="M.RmeAORF2466P"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF09343">
        <property type="protein sequence ID" value="ABF09343"/>
        <property type="gene ID" value="Rmet_2466"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2466"/>
    <dbReference type="eggNOG" id="COG0863">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG2189">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024927_2_1_4"/>
    <dbReference type="OMA" id="WRKANPM"/>
    <dbReference type="OrthoDB" id="1487464at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008170">
        <property type="term" value="F:N-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006306">
        <property type="term" value="P:DNA methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002941">
        <property type="entry name" value="DNA_methylase_N4/N6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002052">
        <property type="entry name" value="DNA_methylase_N6_adenine_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001091">
        <property type="entry name" value="RM_Methyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01555">
        <property type="entry name" value="N6_N4_Mtase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00508">
        <property type="entry name" value="S21N4MTFRASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00092">
        <property type="entry name" value="N6_MTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 7" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N6_N4_Mtase" evidence="6">
        <location>
            <begin position="50"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="289"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU362026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABF09343.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="311" mass="34883" checksum="BFD20D0DBB498D33" modified="2006-05-30" version="1">MRTLPPNTTLPQSEAGGAGSEPFDILDTATMRLYQEDVLEGIKRIPDGSIDLVVADPPYGLGKDYGNDSDLLSGDAYLEWSERWMDAIVPKIAPRGTLYLFCTWQYSPELFVMLKRRMTMINEIIWDRRVPSMGGTTRKFSSVHDNIGFFARQRDYFFDLDPVRIPYDAETKKARSRPRFEGKKWLEVGYNPKDLWSVPRIHRQDPERADHPTQKPLEIVERMVLSSCPPGGIVLDPFTGSGTTAVACVRHGRSFVGFEMNPEYAGLVRERVTAAQPVYAPLKPQASDVADTADAANDDANEAASPVQRLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LKK3</accession>
    <name>Q1LKK3_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">2-keto-D-gluconate reductase (Glyoxalate reductase) (2-ketoaldonate reductase)</fullName>
            <ecNumber evidence="5">1.1.1.26</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">tiaE</name>
        <name evidence="5" type="ordered locus">Rmet_2446</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.26" evidence="5"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09323.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2446"/>
    <dbReference type="EnsemblBacteria" id="ABF09323">
        <property type="protein sequence ID" value="ABF09323"/>
        <property type="gene ID" value="Rmet_2446"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2446"/>
    <dbReference type="eggNOG" id="COG1052">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019796_1_2_4"/>
    <dbReference type="OMA" id="KMKPNCI"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047964">
        <property type="term" value="F:glyoxylate reductase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006139">
        <property type="entry name" value="D-isomer_2_OHA_DH_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029752">
        <property type="entry name" value="D-isomer_DH_CS1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006140">
        <property type="entry name" value="D-isomer_DH_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00389">
        <property type="entry name" value="2-Hacid_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02826">
        <property type="entry name" value="2-Hacid_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00065">
        <property type="entry name" value="D_2_HYDROXYACID_DH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="2-Hacid_dh" evidence="3">
        <location>
            <begin position="8"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="domain" description="2-Hacid_dh_C" evidence="4">
        <location>
            <begin position="113"/>
            <end position="290"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005854"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU003719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF02826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABF09323.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="334" mass="35944" checksum="03C9055DF6F3A3E6" modified="2006-05-30" version="1">MTDMKPSILVTRAIFPDVIERLAQYFDVDDNQEDLVLDSGALRERLAGKVGVLANAADRIDGELVGALPQLRAVCNMAVGYNNLDLPALTAAGIVATNTPDVLTETTADFGWALLMATARRVTEGEHYLRAGKWERWSYDMLVGMDVQGSTLGILGMGRIGQGLARRAAGFDMQVLYHNRSQLPDDVERSLNARYVSKAELLQQSDHLILVLPYSVESHHAIGAAEIAQMKPTATLINLARGGIVDDAALAAALRARKIFAAGLDVFEGEPSVHPDLLTVPNVVLTPHIASASEKTRRAMANLAADNLIAALDVGPQAGKPPTVVNPEVMARRR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-12-12" modified="2022-08-03" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LKM8</accession>
    <name>LEPA_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Elongation factor 4</fullName>
            <shortName evidence="1">EF-4</shortName>
            <ecNumber evidence="1">3.6.5.n1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Ribosomal back-translocase LepA</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">lepA</name>
        <name type="ordered locus">Rmet_2421</name>
    </gene>
    <organism>
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Required for accurate and efficient protein synthesis under certain stress conditions. May act as a fidelity factor of the translation reaction, by catalyzing a one-codon backward translocation of tRNAs on improperly translocated ribosomes. Back-translocation proceeds from a post-translocation (POST) complex to a pre-translocation (PRE) complex, thus giving elongation factor G a second chance to translocate the tRNAs correctly. Binds to ribosomes in a GTP-dependent manner.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>GTP + H2O = GDP + H(+) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="EC" id="3.6.5.n1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell inner membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. LepA subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.5.n1" evidence="1"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09298.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517015.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q1LKM8"/>
    <dbReference type="SMR" id="Q1LKM8"/>
    <dbReference type="STRING" id="266264.Rmet_2421"/>
    <dbReference type="PRIDE" id="Q1LKM8"/>
    <dbReference type="EnsemblBacteria" id="ABF09298">
        <property type="protein sequence ID" value="ABF09298"/>
        <property type="gene ID" value="Rmet_2421"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2421"/>
    <dbReference type="eggNOG" id="COG0481">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009995_3_3_4"/>
    <dbReference type="OMA" id="MVQIAIQ"/>
    <dbReference type="OrthoDB" id="182107at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043022">
        <property type="term" value="F:ribosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045727">
        <property type="term" value="P:positive regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd03709">
        <property type="entry name" value="lepA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.2570">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00071">
        <property type="entry name" value="LepA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006297">
        <property type="entry name" value="EF-4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035647">
        <property type="entry name" value="EFG_III/V"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000640">
        <property type="entry name" value="EFG_V-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004161">
        <property type="entry name" value="EFTu-like_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031157">
        <property type="entry name" value="G_TR_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038363">
        <property type="entry name" value="LepA_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013842">
        <property type="entry name" value="LepA_CTD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035654">
        <property type="entry name" value="LepA_IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000795">
        <property type="entry name" value="T_Tr_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43512">
        <property type="entry name" value="PTHR43512"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00679">
        <property type="entry name" value="EFG_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00009">
        <property type="entry name" value="GTP_EFTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03144">
        <property type="entry name" value="GTP_EFTU_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06421">
        <property type="entry name" value="LepA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00315">
        <property type="entry name" value="ELONGATNFCT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00838">
        <property type="entry name" value="EFG_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="SSF50447"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54980">
        <property type="entry name" value="SSF54980"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01393">
        <property type="entry name" value="lepA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00301">
        <property type="entry name" value="G_TR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51722">
        <property type="entry name" value="G_TR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0997">Cell inner membrane</keyword>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0342">GTP-binding</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000265688" description="Elongation factor 4">
        <location>
            <begin position="1"/>
            <end position="597"/>
        </location>
    </feature>
    <feature type="domain" description="tr-type G">
        <location>
            <begin position="2"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="14"/>
            <end position="19"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="131"/>
            <end position="134"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00071"/>
        </source>
    </evidence>
    <sequence length="597" mass="65943" checksum="0F71BCBF2DC6192C" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LKU5</accession>
    <name>Q1LKU5_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative exported protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_2354</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09231.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_003149757.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2354"/>
    <dbReference type="EnsemblBacteria" id="ABF09231">
        <property type="protein sequence ID" value="ABF09231"/>
        <property type="gene ID" value="Rmet_2354"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2354"/>
    <dbReference type="eggNOG" id="COG1651">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000288_47_6_4"/>
    <dbReference type="OMA" id="KRWVAGN"/>
    <dbReference type="OrthoDB" id="1678187at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012336">
        <property type="entry name" value="Thioredoxin-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13462">
        <property type="entry name" value="Thioredoxin_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="SSF52833"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thioredoxin" evidence="1">
        <location>
            <begin position="43"/>
            <end position="233"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF09231.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="254" mass="27649" checksum="212C116B689B8069" modified="2006-05-30" version="1">MEQKRPSIPMQVQAFRRRRWQFRWPWALAAVLVALLLIWLVSRSPGGSTPQTSAPVSDTQVAGPPWQMGNPEGRFTLTLYADLECPFCRSYFPVLKRWVAGNADVTLQWHHLPLAAHEPAASAEARLAECAGEAGSHAAFWQAVEWVYAHTRSDGQGLPEGLRYPDLTPAIEQCLASERPDAPIRAQTAEATNSGVIATPSLRLHDRETGKAILLQGPIEGDALLSAMDMLAAGDPAAIPTSEMPADVVGDMPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="67" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LLL2</accession>
    <name>Q1LLL2_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Hemerythrin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_2085</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08964.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516799.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2085"/>
    <dbReference type="EnsemblBacteria" id="ABF08964">
        <property type="protein sequence ID" value="ABF08964"/>
        <property type="gene ID" value="Rmet_2085"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2085"/>
    <dbReference type="eggNOG" id="COG3945">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_127112_0_0_4"/>
    <dbReference type="OMA" id="LQQHNMK"/>
    <dbReference type="OrthoDB" id="1701106at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012312">
        <property type="entry name" value="Hemerythrin-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01814">
        <property type="entry name" value="Hemerythrin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Hemerythrin" evidence="1">
        <location>
            <begin position="4"/>
            <end position="126"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF08964.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="148" mass="16346" checksum="BE4FEBC1F2F4F06B" modified="2006-05-30" version="1">MSTTITTLVRQHRDCDEAIEQVEACVRAKDWDAAAGAFARLEFALQGNFSTEEQRLFPAFEQAVGFSNGPTAVMRHEHDEVRGLLDNAREWLELKDVAALAAELDTLVVLLQQHNVKEENVLFPMCQAQVKGLDTLLADFASGPAAHP</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-07-11" modified="2022-05-25" version="67" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LLM6</accession>
    <name>FETP_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Probable Fe(2+)-trafficking protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">Rmet_2071</name>
    </gene>
    <organism>
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Could be a mediator in iron transactions between iron acquisition and iron-requiring processes, such as synthesis and/or repair of Fe-S clusters in biosynthetic enzymes.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the Fe(2+)-trafficking protein family.</text>
    </comment>
    <comment type="sequence caution" evidence="2">
        <conflict type="erroneous initiation">
            <sequence resource="EMBL-CDS" id="ABF08950" version="1"/>
        </conflict>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08950.1"/>
        <property type="status" value="ALT_INIT"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_024570137.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q1LLM6"/>
    <dbReference type="SMR" id="Q1LLM6"/>
    <dbReference type="STRING" id="266264.Rmet_2071"/>
    <dbReference type="EnsemblBacteria" id="ABF08950">
        <property type="protein sequence ID" value="ABF08950"/>
        <property type="gene ID" value="Rmet_2071"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2071"/>
    <dbReference type="eggNOG" id="COG2924">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_170994_0_0_4"/>
    <dbReference type="OrthoDB" id="2038019at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3880.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00686">
        <property type="entry name" value="Fe_traffic_YggX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007457">
        <property type="entry name" value="Fe_traffick_prot_YggX"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036766">
        <property type="entry name" value="Fe_traffick_prot_YggX_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36965">
        <property type="entry name" value="PTHR36965"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04362">
        <property type="entry name" value="Iron_traffic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF029827">
        <property type="entry name" value="Fe_traffic_YggX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111148">
        <property type="entry name" value="SSF111148"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0408">Iron</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000246113" description="Probable Fe(2+)-trafficking protein">
        <location>
            <begin position="1"/>
            <end position="91"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00686"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="91" mass="10298" checksum="3B6844BAF86B5710" modified="2006-07-11" version="2">MARMVHCIKLNKEAEGLDFPPLPGDLGKKIWQNVSKEAWAGWLKHQTMLINENRLNMADPRARQYLIKQTEKYFFGDGADQAAGYVPPPAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LLS5</accession>
    <name>Q1LLS5_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Enoyl-CoA hydratase/isomerase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Rmet_2022</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08901.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2022"/>
    <dbReference type="EnsemblBacteria" id="ABF08901">
        <property type="protein sequence ID" value="ABF08901"/>
        <property type="gene ID" value="Rmet_2022"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2022"/>
    <dbReference type="eggNOG" id="COG1024">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009834_22_1_4"/>
    <dbReference type="OMA" id="GKPYIAF"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003860">
        <property type="term" value="F:3-hydroxyisobutyryl-CoA hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016853">
        <property type="term" value="F:isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029045">
        <property type="entry name" value="ClpP/crotonase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045004">
        <property type="entry name" value="ECH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032259">
        <property type="entry name" value="HIBYL-CoA-H"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43176">
        <property type="entry name" value="PTHR43176"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16113">
        <property type="entry name" value="ECH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52096">
        <property type="entry name" value="SSF52096"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0413">Isomerase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ECH_2" evidence="3">
        <location>
            <begin position="37"/>
            <end position="367"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF16113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF08901.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="383" mass="41480" checksum="5443446B691590FA" modified="2006-05-30" version="1">MLCRGRRFGGSVVESLPAMMETEMTEFVSTQVQGGVAYLTLTRPQALNALSLEMIRAITGALQSWENDPAVHAMVVAGAGGKAFCAGGDIRWFYQAHQANDPLLDQFFVEEYALNHLIHRYAKPYIALMDGVVMGGGMGISQGARLRIVTERTKMAMPETNIGLFPDVGGGWFLARTQGHIGEYLGLTGAVIGAADAIYAKLADAYLPTNAIAEMVASLQARQFTSGEAVLEQIASFTRQHADACVPSTSQLASNAALIDTLFAGASAQAILAAVSDAEGDWAAQTAATLRSRSPLMLCVTLEQIRRARTMSLEDELRMELDMMHDVFRHGDGIEGIRALVIDKDHQPKWNPPRLDEVSGARVRAFFDSPWRKDDHPLATLGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LLX9</accession>
    <name>Q1LLX9_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_1968</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08847.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516692.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1968"/>
    <dbReference type="EnsemblBacteria" id="ABF08847">
        <property type="protein sequence ID" value="ABF08847"/>
        <property type="gene ID" value="Rmet_1968"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1968"/>
    <dbReference type="eggNOG" id="COG3737">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_074390_2_0_4"/>
    <dbReference type="OMA" id="QIREAHF"/>
    <dbReference type="OrthoDB" id="1948784at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036748">
        <property type="entry name" value="MTH938-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007523">
        <property type="entry name" value="NDUFAF3/AAMDC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21192">
        <property type="entry name" value="PTHR21192"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04430">
        <property type="entry name" value="DUF498"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64076">
        <property type="entry name" value="SSF64076"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF08847.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="126" mass="13751" checksum="0335C0DE6446F7CA" modified="2006-05-30" version="1">MKLHADQPQSLNTVTGYGPGYIEINAIRHETAVLVMPEGNVEPWPVSRFEDLEPAHFEALLKKSPEVVLLGTGSTLRFPHPRLTAALSRLHIGVDAMDLQAACRTYNILMAEGRKVAAVLLVEPAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LM36</accession>
    <name>Q1LM36_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">NAD-dependent epimerase/dehydratase:Short-chain dehydrogenase/reductase SDR</fullName>
            <ecNumber evidence="1">1.1.1.100</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_1911</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EC" id="1.1.1.100" evidence="1"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08790.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516644.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1911"/>
    <dbReference type="EnsemblBacteria" id="ABF08790">
        <property type="protein sequence ID" value="ABF08790"/>
        <property type="gene ID" value="Rmet_1911"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1911"/>
    <dbReference type="eggNOG" id="COG1028">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010194_1_3_4"/>
    <dbReference type="OMA" id="NWRGQFA"/>
    <dbReference type="OrthoDB" id="1601931at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004316">
        <property type="term" value="F:3-oxoacyl-[acyl-carrier-protein] reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF08790.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="249" mass="25379" checksum="533CE49198E5C21C" modified="2006-05-30" version="1">MPIHSLSGKVAVIIGGTGGIGLATGHALAEIGASVVLTGRDADRTASAVATLPAGNHLAAHAEVGDSATLRLLADDVQHHYGRVDILVNTSGFTRAIPHADLDALDDALIDEMFAVNWRGQFAAIRAFAPLLRANGDGLVVNVGSIAGRTGQGSNVAYCAVKAGLDVMAMSLGRALAPAIRVLNVSPGVVDTGFVPGRDSSFNERAAKTTPLRRIGQAQDVADAIVACATSLRFATGTTIVVDGGRQLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LM84</accession>
    <name>Q1LM84_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Acyl-CoA synthetase (AMP-forming)/AMP-acid ligase II</fullName>
            <ecNumber evidence="3">6.2.1.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_1863</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EC" id="6.2.1.-" evidence="3"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08742.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1863"/>
    <dbReference type="EnsemblBacteria" id="ABF08742">
        <property type="protein sequence ID" value="ABF08742"/>
        <property type="gene ID" value="Rmet_1863"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1863"/>
    <dbReference type="eggNOG" id="COG0318">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000022_59_5_4"/>
    <dbReference type="OMA" id="FAIPSMG"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016874">
        <property type="term" value="F:ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025110">
        <property type="entry name" value="AMP-bd_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045851">
        <property type="entry name" value="AMP-bd_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020845">
        <property type="entry name" value="AMP-binding_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_Synth/Lig"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13193">
        <property type="entry name" value="AMP-binding_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00455">
        <property type="entry name" value="AMP_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0436">Ligase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AMP-binding" evidence="1">
        <location>
            <begin position="13"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="domain" description="AMP-binding_C" evidence="2">
        <location>
            <begin position="441"/>
            <end position="516"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF08742.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="534" mass="59437" checksum="9003346E7505FCC0" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="104" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LMF9</accession>
    <name>Q1LMF9_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Transcriptional regulator of phenol hydroxylase genes, Fis family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">poxR</name>
        <name evidence="7" type="ordered locus">Rmet_1788</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08667.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516517.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1788"/>
    <dbReference type="EnsemblBacteria" id="ABF08667">
        <property type="protein sequence ID" value="ABF08667"/>
        <property type="gene ID" value="Rmet_1788"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1788"/>
    <dbReference type="eggNOG" id="COG3829">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_119_2_4"/>
    <dbReference type="OMA" id="IYKETEC"/>
    <dbReference type="OrthoDB" id="123059at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002197">
        <property type="entry name" value="HTH_Fis"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002078">
        <property type="entry name" value="Sigma_54_int"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025662">
        <property type="entry name" value="Sigma_54_int_dom_ATP-bd_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025943">
        <property type="entry name" value="Sigma_54_int_dom_ATP-bd_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025944">
        <property type="entry name" value="Sigma_54_int_dom_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004096">
        <property type="entry name" value="V4R"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010523">
        <property type="entry name" value="XylR_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02954">
        <property type="entry name" value="HTH_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00158">
        <property type="entry name" value="Sigma54_activat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02830">
        <property type="entry name" value="V4R"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06505">
        <property type="entry name" value="XylR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01590">
        <property type="entry name" value="HTHFIS"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00989">
        <property type="entry name" value="V4R"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00675">
        <property type="entry name" value="SIGMA54_INTERACT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00676">
        <property type="entry name" value="SIGMA54_INTERACT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00688">
        <property type="entry name" value="SIGMA54_INTERACT_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50045">
        <property type="entry name" value="SIGMA54_INTERACT_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Sigma-54 factor interaction" evidence="6">
        <location>
            <begin position="231"/>
            <end position="460"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50045"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABF08667.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="542" mass="60434" checksum="76BF43E9908DD913" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="100" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LMI8</accession>
    <name>Q1LMI8_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Fused transporter subunits of ABC superfamily: ATP-binding components</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">yheS</name>
        <name evidence="9" type="ordered locus">Rmet_1759</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08638.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516491.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1759"/>
    <dbReference type="EnsemblBacteria" id="ABF08638">
        <property type="protein sequence ID" value="ABF08638"/>
        <property type="gene ID" value="Rmet_1759"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1759"/>
    <dbReference type="eggNOG" id="COG0488">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_36_0_4"/>
    <dbReference type="OMA" id="RYFMDKI"/>
    <dbReference type="OrthoDB" id="1112046at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032781">
        <property type="entry name" value="ABC_tran_Xtn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12848">
        <property type="entry name" value="ABC_tran_Xtn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 7 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="1" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-1278">Translocase</keyword>
    <feature type="domain" description="ABC transporter" evidence="8">
        <location>
            <begin position="2"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="8">
        <location>
            <begin position="313"/>
            <end position="548"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="34"/>
            <end position="41"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="355"/>
            <end position="362"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ABF08638.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="554" mass="60391" checksum="B265788118619952" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LML0</accession>
    <name>Q1LML0_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Outer membrane efflux protein, putative copper/silver efflux system</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Rmet_1737</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the outer membrane factor (OMF) (TC 1.B.17) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08616.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516468.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1737"/>
    <dbReference type="EnsemblBacteria" id="ABF08616">
        <property type="protein sequence ID" value="ABF08616"/>
        <property type="gene ID" value="Rmet_1737"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1737"/>
    <dbReference type="eggNOG" id="COG1538">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012817_13_3_4"/>
    <dbReference type="OMA" id="MAWAFAP"/>
    <dbReference type="OrthoDB" id="1619477at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015562">
        <property type="term" value="F:efflux transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003423">
        <property type="entry name" value="OMP_efflux"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010131">
        <property type="entry name" value="RND_efflux_OM_lipoprot_NodT"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02321">
        <property type="entry name" value="OEP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01845">
        <property type="entry name" value="outer_NodT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0564">Palmitate</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1134">Transmembrane beta strand</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5001442050" evidence="2">
        <location>
            <begin position="26"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="33"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU362097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF08616.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="497" mass="53882" checksum="16EF9744C0F59D8E" modified="2006-05-30" version="1">MKRKPTSLIRPPRWTPALLVPLALAACSLEPTYQRPESPVPQAYPTGPAYESANKGPAGQARPDQATPAASLGWEDFFTDPRLRTLIGLALANNRDLRIATLSIDQARAQYRIQRAAQFPAINANAGFASTRLNSDLRAPGQDAVINSWTAGVGFNAFELDVFGRVRSLKHQALEQYLAIEEVRRSAQISLVAEVASAYLTWQADQELLRLSKETLKVQQDAALMISRSKSAGGMAEIDMHRGQTQVQTAQVDVEQFTRQVAQDENALALLIGGQLPADLPPPQPFANNSYVAELPAGVPSTLMEQRPDIMAAEHQLKGANANIGAARAAFFPQISLTATVGVASTALASLFTGGMAWAFAPQITLPIFNAGANQARLDIATVQKDINVAAYEKTIQGAFREVADGLAARGTYDRQTVAQEALTKEVSETKRLAEIRFKNGVDDYFPVFDAQRQLYDAQKKMVTIELARLTSRASLYKALGGGWSQQTVASLDHPAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LML2</accession>
    <name>Q1LML2_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">General substrate transporter:Major facilitator superfamily MFS_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Rmet_1735</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08614.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516466.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1735"/>
    <dbReference type="EnsemblBacteria" id="ABF08614">
        <property type="protein sequence ID" value="ABF08614"/>
        <property type="gene ID" value="Rmet_1735"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1735"/>
    <dbReference type="eggNOG" id="COG2271">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001265_5_12_4"/>
    <dbReference type="OMA" id="DQMVMSP"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044770">
        <property type="entry name" value="MFS_spinster-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23505">
        <property type="entry name" value="PTHR23505"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="SSF103473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="23"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="62"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="94"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="119"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="156"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="182"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="243"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="275"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="312"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="338"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="373"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="407"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="domain" description="MFS" evidence="6">
        <location>
            <begin position="27"/>
            <end position="427"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABF08614.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="443" mass="46406" checksum="D546AD63593C576D" modified="2006-05-30" version="1">MHGYRSTLAPMAGAPAKARLSRYYPWLVFALSFGLLLSDYMSRQVLNALFPMLKLEWGLSDTELGALGGVVALMVGVLTFPLSILADRWGRVRSLTLMAALWSLATLGCALANSFGEMFLARLCVGIGEAAYGSVGIAVVVSVFPRHLRASLSAAFIAGGAFGSVLGMGLGGVISAHFGWRVAFGGMALFGLILVALYRLLITEKGLLARRQELGEDVSASQTQAKLELRPLVSALFSTRSILCAYVGSALQLFVMASMLAWLPSYFGRYYGMSGAQAGLTAAAFVLIGGAGMIMCGAFTDWIARHIPARKWIVAIAYSVLCCVLLGAAFQLPPGTAQLVLIGAGMLLAGGTAGPASAAVANLTAPAIHGSAFATLTLVNNLLGLAPGPFFTGLIADHIGLRGALQFLPLVGLLAAVCFVIGRRTYSADVHRLERMRAAAGNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="64" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LMN2</accession>
    <name>Q1LMN2_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative membrane protein, putative ion channel</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_1713</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08594.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516446.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1713"/>
    <dbReference type="EnsemblBacteria" id="ABF08594">
        <property type="protein sequence ID" value="ABF08594"/>
        <property type="gene ID" value="Rmet_1713"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1713"/>
    <dbReference type="eggNOG" id="ENOG5032YV7">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_116321_1_1_4"/>
    <dbReference type="OMA" id="VAICLRY"/>
    <dbReference type="OrthoDB" id="1991946at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013099">
        <property type="entry name" value="K_chnl_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07885">
        <property type="entry name" value="Ion_trans_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="47"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="114"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="domain" description="Ion_trans_2" evidence="2">
        <location>
            <begin position="65"/>
            <end position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF08594.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="149" mass="16764" checksum="B6181D2BD9B43541" modified="2006-05-30" version="1">MRVHLLVAAPVMLLCLILQGVVVAICLRRYARYRKRWEGREALWMEIALLMAVMLLTLVCNIVQIAIWAVLFLMLGEFQSFVPALYHSSVNFATLGYGDIVMSERWRMLGPIEAANGILMFGMSTSLMTAAVLDVIKSNMARLMDQAKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="68" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LMU3</accession>
    <name>Q1LMU3_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Inner membrane protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">creD</name>
        <name evidence="3" type="ordered locus">Rmet_1650</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08533.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516378.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1650"/>
    <dbReference type="EnsemblBacteria" id="ABF08533">
        <property type="protein sequence ID" value="ABF08533"/>
        <property type="gene ID" value="Rmet_1650"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1650"/>
    <dbReference type="eggNOG" id="COG4452">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036281_1_0_4"/>
    <dbReference type="OMA" id="RVHPIQY"/>
    <dbReference type="OrthoDB" id="296258at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010364">
        <property type="entry name" value="Uncharacterised_IM_CreD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30092">
        <property type="entry name" value="PTHR30092"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06123">
        <property type="entry name" value="CreD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004548">
        <property type="entry name" value="CreD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="324"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="354"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="379"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="408"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="431"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="461"/>
            <end position="480"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF08533.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="480" mass="52739" checksum="DDE44B4BB2DF8251" modified="2006-05-30" version="1">MKNFRSYPYKILVAAGLILVLWAALQMVFNLVEERSQLQESAEQSIWRSYAGPQTLTGPIIVLPYKEVLVESEAKDAKGRKTQTQYREATRQLLVFPKSLQVQTEIKPSERYRGIHKAIVYEAASQWTGTFVLPDLKAATAFEKSAGHVRFELGEPFVALGVSDMRGLAAAPVMQLAGKSLEMEQGAQLNGLGQGLHAELDLRGAAGKPADARVVPFSLTLSLLGSRSIAFAPVADMNTFSMRSAWPHPSFDGDFLPRQRTVDASGFRAEWSVNALNTHVRERLLPMENGERTQPAVTAPANAAESISVTLIDPVNGYVRVERAIKYGLLFVLLTFAGFYVFEQIKGLRIHPIQYLLVGLALTLFFLMLVSLSEHVTFLLAYLAASAACIGLLAFYLTFVLHSWRRGASFAALIAALYGALYGILVSEDNALLLGSTLLFLVLASIMTVTRRVDWYNGDMQSRSEEEGGEGKSNNGRVAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LN04</accession>
    <name>Q1LN04_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_1589</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08472.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516324.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1589"/>
    <dbReference type="EnsemblBacteria" id="ABF08472">
        <property type="protein sequence ID" value="ABF08472"/>
        <property type="gene ID" value="Rmet_1589"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1589"/>
    <dbReference type="eggNOG" id="COG1262">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012431_9_0_4"/>
    <dbReference type="OMA" id="CRGTFRN"/>
    <dbReference type="OrthoDB" id="1377603at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052699">
        <property type="term" value="P:ergothioneine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1580.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016187">
        <property type="entry name" value="CTDL_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024775">
        <property type="entry name" value="DinB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034660">
        <property type="entry name" value="DinB/YfiT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017806">
        <property type="entry name" value="EgtB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005532">
        <property type="entry name" value="SUMF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042095">
        <property type="entry name" value="SUMF_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12867">
        <property type="entry name" value="DinB_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03781">
        <property type="entry name" value="FGE-sulfatase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109854">
        <property type="entry name" value="SSF109854"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56436">
        <property type="entry name" value="SSF56436"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03440">
        <property type="entry name" value="egtB_TIGR03440"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DinB_2" evidence="2">
        <location>
            <begin position="22"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="domain" description="FGE-sulfatase" evidence="1">
        <location>
            <begin position="187"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="domain" description="FGE-sulfatase" evidence="1">
        <location>
            <begin position="345"/>
            <end position="426"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12867"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF08472.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="429" mass="48724" checksum="47082CFBE57037F2" modified="2006-05-30" version="1">MTSALLPAAASHPPQEPALLSQYRHVRSATEAMVADLSDADATVQSMDDASPAKWHLAHTTWFFEEFVLGARLPDYEPVDPQYRYLFNSYYEAVGARHPRPRRGLLTRPSLDEVLAYREAVDEAMESLLMRAQTDDEAALITLGLHHEQQHQELLLTDILHLFAQNPLRPAYAPTLPSPVIADPRPAPEWIAFPGGVVTIGHSGDGFAFDCEGPRHQVLLHPYLLCSHPVSNHQWFEFIEDGGYQSTGLWLSDGWRWVCDNQIEAPLYWEERDGTWWQMTLRGMQPVDPDSPVTHVSFFEADAFARWAERRLPTEAEWEHAAHNLPATGNFAEGRALRPLPDRQNTAGSLRQMFGDVWEWTGSAFLPYPRFRPREGAVGEYNGKFMSSQMVLRGGSCVTAESHIRASYRNFFYPHQRWQFTGVRLADDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="98" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LN71</accession>
    <name>Q1LN71_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">NAD-reducing hydrogenase diaphorase moiety large subunit</fullName>
            <ecNumber evidence="7">1.12.1.2</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">hoxF</name>
        <name evidence="7" type="ordered locus">Rmet_1522</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the complex I 51 kDa subunit family.</text>
    </comment>
    <dbReference type="EC" id="1.12.1.2" evidence="7"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08405.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516265.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1522"/>
    <dbReference type="EnsemblBacteria" id="ABF08405">
        <property type="protein sequence ID" value="ABF08405"/>
        <property type="gene ID" value="Rmet_1522"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1522"/>
    <dbReference type="eggNOG" id="COG1894">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014881_3_1_4"/>
    <dbReference type="OMA" id="CDQGPAL"/>
    <dbReference type="OrthoDB" id="688908at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047985">
        <property type="term" value="F:hydrogen dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008137">
        <property type="term" value="F:NADH dehydrogenase (ubiquinone) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.1590">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1440.230">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11540">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001949">
        <property type="entry name" value="NADH-UbQ_OxRdtase_51kDa_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011538">
        <property type="entry name" value="Nuo51_FMN-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037225">
        <property type="entry name" value="Nuo51_FMN-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041921">
        <property type="entry name" value="NuoE_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019575">
        <property type="entry name" value="Nuop51_4Fe4S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037207">
        <property type="entry name" value="Nuop51_4Fe4S-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01512">
        <property type="entry name" value="Complex1_51K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10589">
        <property type="entry name" value="NADH_4Fe-4S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00928">
        <property type="entry name" value="NADH_4Fe-4S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140490">
        <property type="entry name" value="SSF140490"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF142019">
        <property type="entry name" value="SSF142019"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="SSF52833"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00644">
        <property type="entry name" value="COMPLEX1_51K_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00645">
        <property type="entry name" value="COMPLEX1_51K_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="4" id="KW-0408">Iron</keyword>
    <keyword evidence="5" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NADH_4Fe-4S" evidence="6">
        <location>
            <begin position="484"/>
            <end position="529"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007523"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABF08405.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="602" mass="66888" checksum="5619558B54DC7B22" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="103" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LN73</accession>
    <name>Q1LN73_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 4">Triosephosphate isomerase</fullName>
            <shortName evidence="3">TIM</shortName>
            <shortName evidence="3">TPI</shortName>
            <ecNumber evidence="3 4">5.3.1.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">Triose-phosphate isomerase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5" type="primary">cbbJ1</name>
        <name evidence="3" type="synonym">tpiA</name>
        <name evidence="5" type="ordered locus">Rmet_1520</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Involved in the gluconeogenesis. Catalyzes stereospecifically the conversion of dihydroxyacetone phosphate (DHAP) to D-glyceraldehyde-3-phosphate (G3P).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3 4">
            <text>D-glyceraldehyde 3-phosphate = dihydroxyacetone phosphate</text>
            <dbReference type="Rhea" id="RHEA:18585"/>
            <dbReference type="ChEBI" id="CHEBI:57642"/>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
            <dbReference type="EC" id="5.3.1.1"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3 4">Carbohydrate biosynthesis; gluconeogenesis.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3 4">Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate from glycerone phosphate: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3 4">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3 4">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 4">Belongs to the triosephosphate isomerase family.</text>
    </comment>
    <dbReference type="EC" id="5.3.1.1" evidence="3 4"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08403.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516263.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1520"/>
    <dbReference type="EnsemblBacteria" id="ABF08403">
        <property type="protein sequence ID" value="ABF08403"/>
        <property type="gene ID" value="Rmet_1520"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1520"/>
    <dbReference type="eggNOG" id="COG0149">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024251_2_1_4"/>
    <dbReference type="OMA" id="IEKNGTM"/>
    <dbReference type="OrthoDB" id="1266295at2"/>
    <dbReference type="UniPathway" id="UPA00109">
        <property type="reaction ID" value="UER00189"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00138"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004807">
        <property type="term" value="F:triose-phosphate isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006094">
        <property type="term" value="P:gluconeogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006096">
        <property type="term" value="P:glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00311">
        <property type="entry name" value="TIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00147_B">
        <property type="entry name" value="TIM_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035990">
        <property type="entry name" value="TIM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022896">
        <property type="entry name" value="TrioseP_Isoase_bac/euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000652">
        <property type="entry name" value="Triosephosphate_isomerase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020861">
        <property type="entry name" value="Triosephosphate_isomerase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21139">
        <property type="entry name" value="PTHR21139"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00121">
        <property type="entry name" value="TIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51351">
        <property type="entry name" value="SSF51351"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00419">
        <property type="entry name" value="tim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00171">
        <property type="entry name" value="TIM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51440">
        <property type="entry name" value="TIM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="1 3 4" id="KW-0312">Gluconeogenesis</keyword>
    <keyword evidence="3 4" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="2 3 4" id="KW-0413">Isomerase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Substrate binding" evidence="3">
        <location>
            <begin position="23"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="3">
        <location>
            <begin position="248"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="active site" description="Electrophile" evidence="3">
        <location>
            <position position="110"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="3">
        <location>
            <position position="182"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="188"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="227"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00147"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU363013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABF08403.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="272" mass="28647" checksum="1CB76F71B276930F" modified="2006-05-30" version="1">MDPTPLPPHPVRGQRRRLLVAGNWKMNGSLAGVQALAAAIADGAVARLDRVQLVLFPPFTYLPEAQRRLGASGMAWGAQNVSGHEDGAFTGEVSAQMLREFGCACVLVGHSERRTLFGENDAVVARKFLAVRQAGMTPVLCVGESLPEREQGATEDVVARQLQAVLDLAGASAFADAVVAYEPVWAIGTGKTATPAQAQDVHAFIRGRLAEADPAIAASLHVLYGGSMKRANARELLAMPDIDGGLLGGASLNVDEFMAVAITAQRLAQENT</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LNV0</accession>
    <name>Q1LNV0_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Hydrogenase expression/formation protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">hoxO</name>
        <name evidence="4" type="ordered locus">Rmet_1291</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the HupG/HyaE family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08176.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516058.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1291"/>
    <dbReference type="EnsemblBacteria" id="ABF08176">
        <property type="protein sequence ID" value="ABF08176"/>
        <property type="gene ID" value="Rmet_1291"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1291"/>
    <dbReference type="eggNOG" id="COG0526">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_144855_0_1_4"/>
    <dbReference type="OMA" id="PGAHCLF"/>
    <dbReference type="OrthoDB" id="2039574at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02965">
        <property type="entry name" value="HyaE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010893">
        <property type="entry name" value="NiFe-hyd_mat_HyaE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07449">
        <property type="entry name" value="HyaE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038934">
        <property type="entry name" value="HyaE_HupG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="SSF52833"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0371">Homeobox</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thioredoxin" evidence="3">
        <location>
            <begin position="10"/>
            <end position="126"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRNR" id="PIRNR038934"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF08176.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="149" mass="16070" checksum="2A11659CDF24CA76" modified="2006-05-30" version="1">MIETTEAHAPLVQRLAREFGATWVDETSVADWSAGGGDRVVLLAGDPVRFPEGVDVAAVLPELMKSFPNRFRIAVVPRDSEDAVAKRYGSQRWPTLLFFRDGQYVTAIAGMQDWDVYLSGVAAALAMPPSRPPTIGIPVVSQTGGSSCH</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="100" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LP99</accession>
    <name>Q1LP99_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Transcriptional regulator, GntR-family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Rmet_1141</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08027.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515928.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1141"/>
    <dbReference type="EnsemblBacteria" id="ABF08027">
        <property type="protein sequence ID" value="ABF08027"/>
        <property type="gene ID" value="Rmet_1141"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1141"/>
    <dbReference type="eggNOG" id="COG2186">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017584_9_1_4"/>
    <dbReference type="OMA" id="EMRLWIE"/>
    <dbReference type="OrthoDB" id="1875458at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07377">
        <property type="entry name" value="WHTH_GntR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.530">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011711">
        <property type="entry name" value="GntR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008920">
        <property type="entry name" value="TF_FadR/GntR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000524">
        <property type="entry name" value="Tscrpt_reg_HTH_GntR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07729">
        <property type="entry name" value="FCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00392">
        <property type="entry name" value="GntR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00035">
        <property type="entry name" value="HTHGNTR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00895">
        <property type="entry name" value="FCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00345">
        <property type="entry name" value="HTH_GNTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48008">
        <property type="entry name" value="SSF48008"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50949">
        <property type="entry name" value="HTH_GNTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH gntR-type" evidence="4">
        <location>
            <begin position="8"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABF08027.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="233" mass="24185" checksum="351DCE1657F155BF" modified="2006-05-30" version="1">MNRLSRPASLASRIAQALRDDIAAGRFAAGARLPAEATLAETFDVSRPIVREAIALLKADGILVTRKGSGAYVSETPGGQVWRVASAPDGGPTLAQLFELRRVVETACAEMAAARRTDVDLVAIRAALATMQTHATDFPSAAAADIAFHHAIAQAAHNPCFTGLTDFVSQQLLVARQHAWENSARLGTLHGTPRAADAEHAALVEAIAAGDPSTARAAATAHLSAAAARMGLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="64" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LPD1</accession>
    <name>Q1LPD1_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_1109</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07995.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515896.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1109"/>
    <dbReference type="EnsemblBacteria" id="ABF07995">
        <property type="protein sequence ID" value="ABF07995"/>
        <property type="gene ID" value="Rmet_1109"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1109"/>
    <dbReference type="HOGENOM" id="CLU_137390_1_1_4"/>
    <dbReference type="OMA" id="KWNRAAI"/>
    <dbReference type="OrthoDB" id="2016615at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10600">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021866">
        <property type="entry name" value="SpoIIAA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038396">
        <property type="entry name" value="SpoIIAA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036513">
        <property type="entry name" value="STAS_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11964">
        <property type="entry name" value="SpoIIAA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52091">
        <property type="entry name" value="SSF52091"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF07995.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="122" mass="13992" checksum="07710BAF309588EE" modified="2006-05-30" version="1">MMEILDGFPANVVAVHGNGKLTRDDYEKVLIPAIDAVLTGREKVRLYYELGPDFTITSMEPGALWDDFKLGVSHYLKWESVVVVTDHDWVRHSINVFRFLVPGQIRTFPMSQSREAREWIVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LPR4</accession>
    <name>Q1LPR4_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Methionine aminotransferase, PLP-dependent</fullName>
            <ecNumber evidence="2">2.6.1.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">ybdL</name>
        <name evidence="2" type="ordered locus">Rmet_0976</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.6.1.-" evidence="2"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07862.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515780.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0976"/>
    <dbReference type="EnsemblBacteria" id="ABF07862">
        <property type="protein sequence ID" value="ABF07862"/>
        <property type="gene ID" value="Rmet_0976"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0976"/>
    <dbReference type="eggNOG" id="COG0436">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017584_4_0_4"/>
    <dbReference type="OMA" id="SQGANQY"/>
    <dbReference type="OrthoDB" id="1462898at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008483">
        <property type="term" value="F:transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009058">
        <property type="term" value="P:biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="SSF53383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aminotran_1_2" evidence="1">
        <location>
            <begin position="35"/>
            <end position="386"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF07862.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="389" mass="42872" checksum="D34E8D6C5822A300" modified="2006-05-30" version="1">MAESNLAPLNPPPSRLPNVGTTIFTVMSALAAEKNAVNLGQGFPDFDCDPRIVDAVTHAMRTGHNQYPPMAGVPQLRQAIAAKIAKLYEHTYDWNTEITVTAGATQGLLTAILCAVHPGDEVIVLEPCYDSYLPAIELAGATAVPVTLEAPLFRIPFDKLAAAITPRTRMIMINTPHNPTGTIWREEDMQQLADLLAGTDILLLSDEVYEHMVYDGQQHESVARHPELARRSFVVSSFGKTYHVTGWKVGFVAAPAALSNEFRKVHQFNVFTVNTPVQYGLADYMADPAPYLNLSAFYQAKRDYFRAGLAGTRFKLLPSDGTYFQCVDYSAISDMSEADFSMWLTREIGVAAIPVSAFYSTPRESGVVRFCFAKKEETLKLALDRLAKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="50" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LQ29</accession>
    <name>Q1LQ29_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_0861</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07747.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515688.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF07747">
        <property type="protein sequence ID" value="ABF07747"/>
        <property type="gene ID" value="Rmet_0861"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0861"/>
    <dbReference type="eggNOG" id="ENOG50343RY">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_196815_0_0_4"/>
    <dbReference type="OMA" id="RHNPFPN"/>
    <dbReference type="OrthoDB" id="2024814at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF07747.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="79" mass="9024" checksum="3927A7FD050A4938" modified="2006-05-30" version="1">MPEYDGFKIIYEVVALPKGKWAIMIEIIRREDGEVLMALHNPFPRQPFDTRLEALDNVNRYVGATIDEFEAASRASRAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LQ60</accession>
    <name>Q1LQ60_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Acyl-CoA transferase/carnitine dehydratase</fullName>
            <ecNumber evidence="1">2.8.3.16</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_0830</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.8.3.16" evidence="1"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07716.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008647133.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0830"/>
    <dbReference type="EnsemblBacteria" id="ABF07716">
        <property type="protein sequence ID" value="ABF07716"/>
        <property type="gene ID" value="Rmet_0830"/>
    </dbReference>
    <dbReference type="GeneID" id="60825316"/>
    <dbReference type="KEGG" id="rme:Rmet_0830"/>
    <dbReference type="eggNOG" id="COG1804">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033975_0_1_4"/>
    <dbReference type="OMA" id="IIAGPYC"/>
    <dbReference type="OrthoDB" id="969868at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033608">
        <property type="term" value="F:formyl-CoA transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1540.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10540">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003673">
        <property type="entry name" value="CoA-Trfase_fam_III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044855">
        <property type="entry name" value="CoA-Trfase_III_dom3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023606">
        <property type="entry name" value="CoA-Trfase_III_dom_1_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02515">
        <property type="entry name" value="CoA_transf_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89796">
        <property type="entry name" value="SSF89796"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF07716.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="406" mass="43950" checksum="1C77710E34529830" modified="2006-05-30" version="1">MGALSHLRVLDLTRVLAGPWCAQNLADFGADVIKIERPGAGDDTRIWGPPWLKTPEGEDTAEAAYYLAANRNKRSVTCDISTPEGQQLVRDLAAQSDVVLENYKVGQLKKYGLDYESLKAIKPDLIYCSVTGFGQTGPYAQRPGYDFIIQGMGGFMSITGERDDLPGGGPQKAGVAISDLMTGQYATIAVLAALAHRDRTGEGQYIDMALLDVQVSMLANMNTNYLASGVPPKRWGNAHPNIVPYQTFQAADGWLIVAVGNDGQFRKFVTAGGRPELADDPRFATNPQRVAHRDVLVPILAEMVAPRTREQWILDLERASVPCGPINTLDEVFENEQVVARGLRVDLPHPSAGTVKLVGSPMKMSATPTEALRHPPLLGEHTDVVLGETLGYSAEHIAALRNKGVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="72" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LQQ3</accession>
    <name>Q1LQQ3_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_0637</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07523.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515494.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0637"/>
    <dbReference type="EnsemblBacteria" id="ABF07523">
        <property type="protein sequence ID" value="ABF07523"/>
        <property type="gene ID" value="Rmet_0637"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0637"/>
    <dbReference type="eggNOG" id="COG3521">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_092347_0_0_4"/>
    <dbReference type="OMA" id="PMETSAQ"/>
    <dbReference type="OrthoDB" id="1864732at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.4150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017734">
        <property type="entry name" value="T6SS_SciN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038706">
        <property type="entry name" value="Type_VI_SciN-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37625">
        <property type="entry name" value="PTHR37625"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12790">
        <property type="entry name" value="T6SS-SciN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03352">
        <property type="entry name" value="VI_chp_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="170"/>
            <end position="189"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF07523.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="189" mass="20210" checksum="CE7BD307298B303C" modified="2006-05-30" version="1">MKQALRNGGIALVATLTVAGCGVGQAVKNSTVEAAKWAFTTQVKTMNLDLISRSSLNTNSAGESLSTVVRIYQLKTPQAFEQLSYAQLQTHDLASLKADLLASKDVVLRPAASVSLAEPMQEDCEYVGVAALFRDAGNNAVWKLVIPKKQWKKTDPVKIEARGNVLERVGATPEALRPQASQLGATSDT</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="72" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LQS1</accession>
    <name>Q1LQS1_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_0619</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07505.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515476.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0619"/>
    <dbReference type="EnsemblBacteria" id="ABF07505">
        <property type="protein sequence ID" value="ABF07505"/>
        <property type="gene ID" value="Rmet_0619"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0619"/>
    <dbReference type="eggNOG" id="COG3157">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_116190_0_0_4"/>
    <dbReference type="OMA" id="GTRVHKP"/>
    <dbReference type="OrthoDB" id="1153621at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.110.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036624">
        <property type="entry name" value="Hcp1-lik_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008514">
        <property type="entry name" value="T6SS_Hcp"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05638">
        <property type="entry name" value="T6SS_HCP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF141452">
        <property type="entry name" value="SSF141452"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03344">
        <property type="entry name" value="VI_effect_Hcp1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF07505.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="161" mass="18255" checksum="DB5D173BBC936559" modified="2006-05-30" version="1">MAIPAYMWIKDDGGADIKGSVTVTEREGSVEVVAFDHSVNIPTDSNTGKLTGTRVHKPILFTKETDASTPYLYKAVTSGQTLKSIEIKWYKIDDAGKEKEYFNTKLENVKVVGVTAKMLDIKNPAYEKHNHLEDVELRYERITWSYKDGNIIHADSWNERS</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LQT9</accession>
    <name>Q1LQT9_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Multidrug resistance protein mdtA (Multidrug transporter mdtA)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">mdtA</name>
        <name evidence="6" type="ordered locus">Rmet_0601</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07487.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0601"/>
    <dbReference type="EnsemblBacteria" id="ABF07487">
        <property type="protein sequence ID" value="ABF07487"/>
        <property type="gene ID" value="Rmet_0601"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0601"/>
    <dbReference type="eggNOG" id="COG0845">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018816_2_0_4"/>
    <dbReference type="OMA" id="NTAQMRT"/>
    <dbReference type="OrthoDB" id="1432684at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032317">
        <property type="entry name" value="HlyD_D23"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006143">
        <property type="entry name" value="RND_pump_MFP"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16576">
        <property type="entry name" value="HlyD_D23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01730">
        <property type="entry name" value="RND_mfp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="56"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="domain" description="HlyD_D23" evidence="5">
        <location>
            <begin position="112"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="424"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="160"/>
            <end position="187"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF16576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABF07487.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="459" mass="49077" checksum="C6FA862EF4D42C6B" modified="2006-05-30" version="1">MEAHARARGLTRESHREPEIISNKAVISHMTATDYACRVKRPMSRMSASPPPRRRPLAIALYVVIAIVVVWLVARPLGRWLFPPKAPQANPAIVVTTAAAQGEDVDIGLTGVGTVQAMQTVTVKPRVDGQLERVGFVEGQDVKEGQVLAQIDPRTFQAQLQQAQATKARDEAQLANARLDLKRYEELIKVDSTTRQTLDTQRALVDQLAAAVRNDEAQIALDKVQLDFTTITAPLSGRVGARLVDPGNIVHATDTTGLVVIRQIDPIDVVFTLPDDAFGRINRAMQASQSPLVVFAHERGTDADLGHGGLILLNNTIDTTSGTIQLKARFPNPKHLLWPGQYVNARLVLGQRDRAVVIPASAVQRNDAGTYVYAVQADQTVKLQPVEVAELQGARAVIAKGVQAGERVVADGQYKLKPGSLIAEAPARPGTAPTGGAAAESTAATSPAVAASATAAVRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="98" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LQU1</accession>
    <name>Q1LQU1_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Nitrous-oxide reductase</fullName>
            <ecNumber evidence="7">1.7.2.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">N(2)OR</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">N2O reductase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ordered locus">Rmet_0599</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Nitrous-oxide reductase is part of a bacterial respiratory system which is activated under anaerobic conditions in the presence of nitrate or nitrous oxide.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>2 Fe(III)-[cytochrome c] + H2O + N2 = 2 Fe(II)-[cytochrome c] + 2 H(+) + nitrous oxide</text>
            <dbReference type="Rhea" id="RHEA:43108"/>
            <dbReference type="Rhea" id="RHEA-COMP:10350"/>
            <dbReference type="Rhea" id="RHEA-COMP:14399"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17045"/>
            <dbReference type="ChEBI" id="CHEBI:17997"/>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
            <dbReference type="EC" id="1.7.2.4"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Nitrogen metabolism; nitrate reduction (denitrification); dinitrogen from nitrate: step 4/4.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Periplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the NosZ family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">In the C-terminal section; belongs to the cytochrome c oxidase subunit 2 family.</text>
    </comment>
    <dbReference type="EC" id="1.7.2.4" evidence="7"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07485.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515458.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0599"/>
    <dbReference type="EnsemblBacteria" id="ABF07485">
        <property type="protein sequence ID" value="ABF07485"/>
        <property type="gene ID" value="Rmet_0599"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0599"/>
    <dbReference type="eggNOG" id="COG1622">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_163365_0_0_4"/>
    <dbReference type="OMA" id="IRVHARK"/>
    <dbReference type="OrthoDB" id="1654242at2"/>
    <dbReference type="UniPathway" id="UPA00652">
        <property type="reaction ID" value="UER00709"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042597">
        <property type="term" value="C:periplasmic space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004129">
        <property type="term" value="F:cytochrome-c oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050304">
        <property type="term" value="F:nitrous-oxide reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019333">
        <property type="term" value="P:denitrification pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.420">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002429">
        <property type="entry name" value="CcO_II-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008972">
        <property type="entry name" value="Cupredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028096">
        <property type="entry name" value="EfeO_Cupredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006311">
        <property type="entry name" value="TAT_signal"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13473">
        <property type="entry name" value="Cupredoxin_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49503">
        <property type="entry name" value="SSF49503"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50857">
        <property type="entry name" value="COX2_CUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51318">
        <property type="entry name" value="TAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="COX2_CUA" evidence="12">
        <location>
            <begin position="30"/>
            <end position="124"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004779"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006790"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00010372"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00011896"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00016560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00029370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00031077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00032847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ABF07485.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="124" mass="13149" checksum="FFD98123C065D7BF" modified="2006-05-30" version="1">MNTANASRRTLLGWMLAAAVVPVVSVVSRSDAAAPRVIPMRARRFVFIPDRVKLKAGETVVLSITAEDVVMGFSAPDFDVRADLPPGQAIKVTLTAGKPGSYGFLCDIFCGSGHENMSGLIEVT</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LQZ8</accession>
    <name>Q1LQZ8_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_0542</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07428.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515404.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0542"/>
    <dbReference type="EnsemblBacteria" id="ABF07428">
        <property type="protein sequence ID" value="ABF07428"/>
        <property type="gene ID" value="Rmet_0542"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0542"/>
    <dbReference type="eggNOG" id="COG0208">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_057296_0_0_4"/>
    <dbReference type="OMA" id="NAYVMQD"/>
    <dbReference type="OrthoDB" id="1384440at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.620.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025859">
        <property type="entry name" value="AurF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009078">
        <property type="entry name" value="Ferritin-like_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012348">
        <property type="entry name" value="RNR-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11583">
        <property type="entry name" value="AurF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47240">
        <property type="entry name" value="SSF47240"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF07428.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="360" mass="41471" checksum="30348167A5DBFF36" modified="2006-05-30" version="1">MTSMHRYTLPVDQTGWSVGSQVATEFNWEYDNGSSKLLQLYETSKKQQWNASDRIDWSQELDPENPQELDDRMIPIYGTKLWDKLGHNDRIEVRRHQQAHSLSQFLHGEQGALMVSARIVQMVPGMDAKFYAATQTMDEARHVEAYSRLLHEKVGLLYPITPGLKSLLETTLTDSRWDFCYLGMQVLVEGLALAAFQRIRDFAKNPLAASINAYVMQDESRHVAFGRTALRGYYPQLTEAEQREREDFVIEACQLMRERFDQKEVWGRLGLPEDECAQAVRDSETMRLFRQRLFSRIVPTVKDIGLWSQRVQDAFAAMGAIEFANVDVEAQFANDHKVAEEFDARRHVMDTIKQASDSAH</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LR32</accession>
    <name>Q1LR32_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">D-Alanyl-D-alanine carboxypeptidase, S13 family</fullName>
            <ecNumber evidence="2">3.4.16.4</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">dacB</name>
        <name evidence="2" type="ordered locus">Rmet_0508</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase S13 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.16.4" evidence="2"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07394.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515373.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0508"/>
    <dbReference type="MEROPS" id="S13.003"/>
    <dbReference type="EnsemblBacteria" id="ABF07394">
        <property type="protein sequence ID" value="ABF07394"/>
        <property type="gene ID" value="Rmet_0508"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0508"/>
    <dbReference type="eggNOG" id="COG2027">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017692_2_1_4"/>
    <dbReference type="OMA" id="DGTMRKR"/>
    <dbReference type="OrthoDB" id="720131at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009002">
        <property type="term" value="F:serine-type D-Ala-D-Ala carboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.710.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012338">
        <property type="entry name" value="Beta-lactam/transpept-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000667">
        <property type="entry name" value="Peptidase_S13"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30023">
        <property type="entry name" value="PTHR30023"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02113">
        <property type="entry name" value="Peptidase_S13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00922">
        <property type="entry name" value="DADACBPTASE3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56601">
        <property type="entry name" value="SSF56601"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0121">Carboxypeptidase</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006096"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF07394.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="518" mass="55434" checksum="821D622FD22808C0" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LR83</accession>
    <name>Q1LR83_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Stress response kinase A</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">Serine/threonine-protein kinase SrkA</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="4" type="primary">yihE</name>
        <name evidence="2" type="synonym">srkA</name>
        <name evidence="4" type="ordered locus">Rmet_0457</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">A protein kinase that phosphorylates Ser and Thr residues. Probably acts to suppress the effects of stress linked to accumulation of reactive oxygen species. Probably involved in the extracytoplasmic stress response.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>ATP + L-seryl-[protein] = ADP + H(+) + O-phospho-L-seryl-[protein]</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>ATP + L-threonyl-[protein] = ADP + H(+) + O-phospho-L-threonyl-[protein]</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="2">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SrkA/RdoA protein kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07343.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515331.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0457"/>
    <dbReference type="EnsemblBacteria" id="ABF07343">
        <property type="protein sequence ID" value="ABF07343"/>
        <property type="gene ID" value="Rmet_0457"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0457"/>
    <dbReference type="eggNOG" id="COG2334">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_054715_0_0_4"/>
    <dbReference type="OMA" id="MHYSAWL"/>
    <dbReference type="OrthoDB" id="1003984at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106310">
        <property type="term" value="F:protein serine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006468">
        <property type="term" value="P:protein phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01497">
        <property type="entry name" value="SrkA_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002575">
        <property type="entry name" value="Aminoglycoside_PTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032882">
        <property type="entry name" value="SrkA/RdoA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39573">
        <property type="entry name" value="PTHR39573"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01636">
        <property type="entry name" value="APH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="SSF56112"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2" id="KW-0418">Kinase</keyword>
    <keyword evidence="2" id="KW-0460">Magnesium</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="1 2" id="KW-0346">Stress response</keyword>
    <keyword evidence="2 4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="APH" evidence="3">
        <location>
            <begin position="42"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="2">
        <location>
            <position position="215"/>
        </location>
    </feature>
    <feature type="active site" evidence="2">
        <location>
            <position position="241"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="220"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="241"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="site" description="ATP" evidence="2">
        <location>
            <position position="43"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01497"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01636"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF07343.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="348" mass="38922" checksum="3188A43BA27741C1" modified="2006-05-30" version="1">MSEPDRPSSTDEVPYAGLTPECILDALEAAGFYPDGRLLALNSYENRVWQVGIEDAAPVVAKFYRPGRWTDAAILEEHAFVQQLAGAEVPAVPALAANSGDQSGTTLFTHEGFRFAVFPRCGGREPALDKAETRTWLGRFIGRIHAIGATAPYQARPALDIDTFGIASRDYLLAHDCIPADLLAPWRAAADLALDGVRRSYERAGEVRLLRLHGDCHRGNVLWIDEEDARGRGTPGPHFVDFDDSRMGPAVQDLWMLLEGDRVAMQDQLADIVAGYEDFAEFDARELWLVEALRTLRLLHYSAWLASRWRDPAFPAAFPWFGTARYWQDRILELREQIALMDEAPLWA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LRQ8</accession>
    <name>Q1LRQ8_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Peptidase M16-like protein (Zn-dependent peptidase)</fullName>
            <ecNumber evidence="7">3.4.-.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Rmet_0282</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 3">Belongs to the peptidase M16 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.-.-" evidence="7"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07168.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515172.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0282"/>
    <dbReference type="MEROPS" id="M16.019"/>
    <dbReference type="EnsemblBacteria" id="ABF07168">
        <property type="protein sequence ID" value="ABF07168"/>
        <property type="gene ID" value="Rmet_0282"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0282"/>
    <dbReference type="eggNOG" id="COG0612">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009902_1_0_4"/>
    <dbReference type="OMA" id="FFEHLLF"/>
    <dbReference type="OrthoDB" id="1188251at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011249">
        <property type="entry name" value="Metalloenz_LuxS/M16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011765">
        <property type="entry name" value="Pept_M16_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001431">
        <property type="entry name" value="Pept_M16_Zn_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007863">
        <property type="entry name" value="Peptidase_M16_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00675">
        <property type="entry name" value="Peptidase_M16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05193">
        <property type="entry name" value="Peptidase_M16_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63411">
        <property type="entry name" value="SSF63411"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00143">
        <property type="entry name" value="INSULINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase_M16" evidence="5">
        <location>
            <begin position="83"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase_M16_C" evidence="6">
        <location>
            <begin position="239"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="37"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="39"/>
            <end position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU004447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00675"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF05193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABF07168.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="504" mass="56581" checksum="742BD3BE3B69BEE2" modified="2006-05-30" version="1">MNHCADVSGAGVAKFPRRILSSLILASLIGPLAGTAWSQTPGEAPTTATSASPRQGPPPTQPTATVGQAQAGTTEFRLSNGLRVIVKEDHRAPTVAHMIFYRVGGFDEVSGTTGVAHMLEHMMFKGTPKVPVGEFSRQIALLGGRENALTNRDFTLYYQQISKQYLPKMMELEADRMANLIFKKEEFEREMKVVMEERRLRTDDSPRGTVYEQLLATVYTAMPYRHPVIGWMDDLVNMRVEDVHDWYKTWYVPNNAMVIVTGDVKPDEVRALAERYYGKLKPHPLPLRKTQIEAPQKGIKRIWVKAPAENPYMVMAYKVPRLRDVEKDVDPYALEVLSAVLNGYDNARLTRELVRERRLADDVNVGYDSINRADSLFVLDGTPANGHTTEEIEAALREEIQRIAKNGVSEEELKRVKAQVVAGQIYKRDSVFGQGMEIGVSEISEISWRQIDRMLDKIKEVTPAQVQAVAAKYFSDDNLTVATLLPQPIDPNKPKTPVPEGLRH</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LRS2</accession>
    <name>Q1LRS2_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_0268</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07154.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0268"/>
    <dbReference type="EnsemblBacteria" id="ABF07154">
        <property type="protein sequence ID" value="ABF07154"/>
        <property type="gene ID" value="Rmet_0268"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0268"/>
    <dbReference type="eggNOG" id="COG1999">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_109681_0_0_4"/>
    <dbReference type="OMA" id="YFMRQIR"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="SSF52833"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="40"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF07154.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="225" mass="25581" checksum="6456493B7D159C8F" modified="2006-05-30" version="1">METYKRTMNAQTNAMPQPDPTASPDPRIDARTRRGRLQMLMLLLVCASPVLFSYFTYYVIKPTGGSTNYGVLIDPQRPMPPVQVTNERNESVPLASMRGKWLMVMTDPSACDETCAKKLFTVRQIRVGQGEDRERIVPVWLIQDRGDVDDRLAAAYNEPYAGVRFLRMDRATIAQWLPAEDGARVEDAIYLVDPLGNLMMRWPKDPDPKKISSDLKKLLKYSRIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="118" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LRS8</accession>
    <name>Q1LRS8_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Cytochrome c oxidase subunit 1</fullName>
            <ecNumber evidence="8">7.1.1.9</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">coxA</name>
        <name evidence="11" type="ordered locus">Rmet_0262</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Cytochrome c oxidase is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Subunits 1-3 form the functional core of the enzyme complex. CO I is the catalytic subunit of the enzyme. Electrons originating in cytochrome c are transferred via the copper A center of subunit 2 and heme A of subunit 1 to the bimetallic center formed by heme A3 and copper B.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6 8">
            <text>4 Fe(II)-[cytochrome c] + 8 H(+)(in) + O2 = 4 Fe(III)-[cytochrome c] + 4 H(+)(out) + 2 H2O</text>
            <dbReference type="Rhea" id="RHEA:11436"/>
            <dbReference type="Rhea" id="RHEA-COMP:10350"/>
            <dbReference type="Rhea" id="RHEA-COMP:14399"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
            <dbReference type="EC" id="7.1.1.9"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 8">Energy metabolism; oxidative phosphorylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cell membrane</location>
            <topology evidence="8">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the heme-copper respiratory oxidase family.</text>
    </comment>
    <dbReference type="EC" id="7.1.1.9" evidence="8"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07148.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515154.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0262"/>
    <dbReference type="EnsemblBacteria" id="ABF07148">
        <property type="protein sequence ID" value="ABF07148"/>
        <property type="gene ID" value="Rmet_0262"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0262"/>
    <dbReference type="eggNOG" id="COG0843">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011899_7_3_4"/>
    <dbReference type="OMA" id="PVDFQYH"/>
    <dbReference type="OrthoDB" id="316745at2"/>
    <dbReference type="UniPathway" id="UPA00705"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045277">
        <property type="term" value="C:respiratory chain complex IV"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004129">
        <property type="term" value="F:cytochrome-c oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015990">
        <property type="term" value="P:electron transport coupled proton transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006119">
        <property type="term" value="P:oxidative phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd01663">
        <property type="entry name" value="Cyt_c_Oxidase_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.210.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023616">
        <property type="entry name" value="Cyt_c_oxase-like_su1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036927">
        <property type="entry name" value="Cyt_c_oxase-like_su1_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000883">
        <property type="entry name" value="Cyt_C_Oxase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023615">
        <property type="entry name" value="Cyt_c_Oxase_su1_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033944">
        <property type="entry name" value="Cyt_c_oxase_su1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014241">
        <property type="entry name" value="Cyt_c_oxidase_su1_bac"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10422">
        <property type="entry name" value="PTHR10422"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00115">
        <property type="entry name" value="COX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01165">
        <property type="entry name" value="CYCOXIDASEI"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81442">
        <property type="entry name" value="SSF81442"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02891">
        <property type="entry name" value="CtaD_CoxA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50855">
        <property type="entry name" value="COX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00077">
        <property type="entry name" value="COX1_CUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-0186">Copper</keyword>
    <keyword evidence="7" id="KW-0249">Electron transport</keyword>
    <keyword evidence="7" id="KW-0349">Heme</keyword>
    <keyword evidence="8" id="KW-0408">Iron</keyword>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0679">Respiratory chain</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="7" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="44"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="85"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="127"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="170"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="206"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="266"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="296"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="327"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="359"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="399"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="434"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="474"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="domain" description="COX1" evidence="10">
        <location>
            <begin position="32"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00029368"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU363061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50855"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ABF07148.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="536" mass="59846" checksum="06C3AC882908B242" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LS65</accession>
    <name>Q1LS65_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Cobalamin (Vitamin B12) synthesis</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">cobW</name>
        <name evidence="4" type="ordered locus">Rmet_0125</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SIMIBI class G3E GTPase family. CobW subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07011.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515043.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0125"/>
    <dbReference type="EnsemblBacteria" id="ABF07011">
        <property type="protein sequence ID" value="ABF07011"/>
        <property type="gene ID" value="Rmet_0125"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0125"/>
    <dbReference type="eggNOG" id="COG0523">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017452_2_0_4"/>
    <dbReference type="OrthoDB" id="1267701at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1220.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011629">
        <property type="entry name" value="Cbl_biosynth_CobW-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036627">
        <property type="entry name" value="CobW-likC_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003495">
        <property type="entry name" value="CobW/HypB/UreG_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02492">
        <property type="entry name" value="cobW"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07683">
        <property type="entry name" value="CobW_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00833">
        <property type="entry name" value="CobW_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CobW C-terminal" evidence="3">
        <location>
            <begin position="215"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="363"/>
            <end position="391"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF07011.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="391" mass="42228" checksum="6D4F8BEFFFB2348E" modified="2006-05-30" version="1">MAVRLPVTVLSGFTGAGKTTVLRHLEAQAGARVAVVDSHGDLLAEVERLAQTGQYDHVVIEAGATDEPLGLAEGFAFEDEHGETTSVGHLDTLVTVIDASRFLQDYHDADFLSARALAAHDDDDRTVVDVLIDQVEVCDVLVVNKIDLVDAGQLGRLHVMLHALNPRADIVDASHGQVPAARVLDTGRFDIDATPNAAGWQAALQGDTVPDAAGVSTLVYRRRRPFHPQRFADLIHTEWMREHGDVLRSKGLFWLASRMDIAGDWSQAGGVCRPGAAGAWWAAIDAEEWPPEGPARAAIEADLIEDGLPAEYGDRRQELVLIGLDLDAHALEAQLDACLLTDDEMAAGPEAWAAYPDPFPDWGNAFDDDHDHDHGHDHDHGDGPCDCGHAH</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="72" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LSB3</accession>
    <name>Q1LSB3_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_0077</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF06963.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515001.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0077"/>
    <dbReference type="EnsemblBacteria" id="ABF06963">
        <property type="protein sequence ID" value="ABF06963"/>
        <property type="gene ID" value="Rmet_0077"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0077"/>
    <dbReference type="eggNOG" id="COG1633">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1018917_0_0_4"/>
    <dbReference type="OMA" id="DFCAFMS"/>
    <dbReference type="OrthoDB" id="644190at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.620.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009078">
        <property type="entry name" value="Ferritin-like_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012348">
        <property type="entry name" value="RNR-like"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47240">
        <property type="entry name" value="SSF47240"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF06963.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="286" mass="34003" checksum="9048E9A2A18C4F18" modified="2006-05-30" version="1">MLYPELFKSLEAVRWHMDKDIPWDTFDASLLTDDQAKTIRMNAITEWSALPATEMFLRDNRHDSDFSAFMSIWFFEEQKHSLVLMEYLRRFRPDLVPTEEELHAVRFEFDPAPPLETLMLHFCGEIRLNHWYRRAAEWHSEPVIKHIYRTLSQDEARHGGAYLRYMKKYLNQFGDNARAAFAKIGVLMASARRTEKPLHPTNLHVNKALFPNDTVQSRLPDPDWLEHWLDEQIRFDEIWEKKVIERILHNMSLLFERTFDTVQDLNRYRKELNISLQSGGAQPAQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="109" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LSG2</accession>
    <name>Q1LSG2_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Response regulator, OmpR-family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Rmet_0028</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF06914.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0028"/>
    <dbReference type="EnsemblBacteria" id="ABF06914">
        <property type="protein sequence ID" value="ABF06914"/>
        <property type="gene ID" value="Rmet_0028"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0028"/>
    <dbReference type="eggNOG" id="COG0745">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_30_0_4"/>
    <dbReference type="OMA" id="CIEVDNF"/>
    <dbReference type="OrthoDB" id="817710at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00383">
        <property type="entry name" value="trans_reg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001867">
        <property type="entry name" value="OmpR/PhoB-type_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016032">
        <property type="entry name" value="Sig_transdc_resp-reg_C-effctor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039420">
        <property type="entry name" value="WalR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48111">
        <property type="entry name" value="PTHR48111"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00486">
        <property type="entry name" value="Trans_reg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00862">
        <property type="entry name" value="Trans_reg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46894">
        <property type="entry name" value="SSF46894"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51755">
        <property type="entry name" value="OMPR_PHOB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Response regulatory" evidence="5">
        <location>
            <begin position="7"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="domain" description="OmpR/PhoB-type" evidence="6">
        <location>
            <begin position="134"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="OmpR/PhoB-type" evidence="3">
        <location>
            <begin position="134"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="236"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="239"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="2">
        <location>
            <position position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABF06914.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="268" mass="30186" checksum="E24395C5AA5E4A5A" modified="2006-05-30" version="1">MTRMGLRVASLEDDAAEAARIREIVTAEGHECVTFADGRRMLVTLRKVTFDLLLLDWHVPHVSGREVLDWVRAHLDPRIPVMFLTGRDTEADIVATLSAGADDYMVKPIRPRELGARIAALLRRAYPDSRQPDDGRLTWGCFRFDAVTREATCHGQEVRLTPKEFEVALLLFRNEGRAVPREYMLAAIWGKELPPLSRTIDTHVSRVRTKLGLWEHNGVRLQPLYTHGYRLELLTTPGMAPPSTPPPTPPSTPPSQKKKGTRRCLSAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-04-26" modified="2021-09-29" version="72" xmlns="http://uniprot.org/uniprot">
    <accession>Q58AL8</accession>
    <name>Q58AL8_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">CzcJ, involved in Cd(II), Zn(II), Co(II) resistance</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">czcJ</name>
        <name evidence="2" type="ordered locus">Rmet_5975</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2 3">
        <name>pMOL30</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid pMOL30</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000354">
        <property type="protein sequence ID" value="ABF12834.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008646716.1">
        <property type="nucleotide sequence ID" value="NC_007971.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_145600.1">
        <property type="nucleotide sequence ID" value="NC_006466.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF12834">
        <property type="protein sequence ID" value="ABF12834"/>
        <property type="gene ID" value="Rmet_5975"/>
    </dbReference>
    <dbReference type="GeneID" id="60820439"/>
    <dbReference type="KEGG" id="rme:Rmet_5975"/>
    <dbReference type="HOGENOM" id="CLU_136209_5_1_4"/>
    <dbReference type="OMA" id="MQLLRHT"/>
    <dbReference type="OrthoDB" id="1929461at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid pMOL30"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5013531910" evidence="1">
        <location>
            <begin position="24"/>
            <end position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF12834.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="92" mass="9594" checksum="11B21D33C950F621" modified="2005-04-26" version="1">MKTIHTALIAAALTIAAAGSAIAATPDGQIHRPRDPYTDGGRAAVNDIYAGGARIDKRDVFTDGARLGQRDVFTDGSRIGPRDVFTDGARGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-01-04" modified="2021-09-29" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>Q5NV13</accession>
    <name>Q5NV13_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_6247</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2 3">
        <name>pMOL28</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid pMOL28</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000355">
        <property type="protein sequence ID" value="ABF13106.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011239938.1">
        <property type="nucleotide sequence ID" value="NC_007972.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_161677.1">
        <property type="nucleotide sequence ID" value="NC_006525.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF13106">
        <property type="protein sequence ID" value="ABF13106"/>
        <property type="gene ID" value="Rmet_6247"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6247"/>
    <dbReference type="HOGENOM" id="CLU_128651_0_0_4"/>
    <dbReference type="OMA" id="ARIMAEW"/>
    <dbReference type="OrthoDB" id="2015659at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid pMOL28"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="44"/>
            <end position="67"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF13106.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="149" mass="17703" checksum="9D71F4E6E087B1DD" modified="2005-01-04" version="1">MRFTRFGRHSPINFNTRRQAAFARKQQRERERYPLFAEHVAAEQHSADDELARRQRRSDRLEASTRDHQARVWRQKRAVYFSLTPEQQAEVRAKWLAWTGPTTAFYFAYIVDNISGEAARRDEVLRARTLEVRRRVLANMPEQVALEMA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-01-04" modified="2022-08-03" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q5NV46</accession>
    <name>Q5NV46_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Conjugal transfer protein (Entry exclusion, mating pair formation)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">trbJ</name>
        <name evidence="2" type="ordered locus">Rmet_6306</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2 3">
        <name>pMOL28</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid pMOL28</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000355">
        <property type="protein sequence ID" value="ABF13165.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008649459.1">
        <property type="nucleotide sequence ID" value="NC_007972.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_161635.1">
        <property type="nucleotide sequence ID" value="NC_006525.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF13165">
        <property type="protein sequence ID" value="ABF13165"/>
        <property type="gene ID" value="Rmet_6306"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6306"/>
    <dbReference type="HOGENOM" id="CLU_1122933_0_0_4"/>
    <dbReference type="OMA" id="YQMMANQ"/>
    <dbReference type="OrthoDB" id="1557327at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid pMOL28"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5013532137" evidence="1">
        <location>
            <begin position="23"/>
            <end position="252"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF13165.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="252" mass="27774" checksum="8266A6575226FB47" modified="2005-01-04" version="1">MKKWIANLAVAASVLGAAPAHAALPVTDWVGLVQTTISALQSIKSEVYENTNIVYQYQMMANQLLQATGLDAEAITEQFNSIKDEIGKYEVYGTTLKDLYGTVSDNADYLKKIQSMVVSSGKTKEQWFEDQRSLLTNGDKTAKALFSLGEQIFKNTQSVAKRRQKIQSQIKLSATAQAAAQTTNQMLDVLASQNSDLLQLMSVRAQADADRDQQTVAKETQSAEAMRSLAAAQDEELKNLRARVFSRKLQSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-03-23" modified="2020-10-07" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>D3DXK1</accession>
    <name>D3DXK1_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_6403</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ADC45021.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_6403"/>
    <dbReference type="EnsemblBacteria" id="ADC45021">
        <property type="protein sequence ID" value="ADC45021"/>
        <property type="gene ID" value="Rmet_6403"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6403"/>
    <dbReference type="HOGENOM" id="CLU_2772940_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADC45021.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="69" mass="7787" checksum="6D968886BA34D40D" modified="2010-03-23" version="1">MSSALPFIPLFFVALSLPVRALAPERRVLLRTIFRVRRHCVAANQRNCTKSPPEALLLLGFMSERSAFC</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-03-23" modified="2020-10-07" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>D3DXR5</accession>
    <name>D3DXR5_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_6477</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ADC45085.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADC45085">
        <property type="protein sequence ID" value="ADC45085"/>
        <property type="gene ID" value="Rmet_6477"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6477"/>
    <dbReference type="HOGENOM" id="CLU_1440001_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="35"/>
            <end position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADC45085.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="188" mass="19986" checksum="A71402A45E2B4F15" modified="2010-03-23" version="1">MTTRTTYCYLLLLLLLLLLLLLLLLLLLLLLLLLLLLLLLLLLLLLLLLLLLLLLLLRASASSTALLLLLAASTSGAVRRRGGYAGAVQAGQIHAGRAMGAIGIGVAFTDAVRAVGVRVALTDAMCAIGVRVTLADVHFIAITVGRYASVLQALRGLCHGQRAGAEKSRCDGRGNQGFQHFLVLVNFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-03-23" modified="2020-10-07" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>D3DXV3</accession>
    <name>D3DXV3_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_6515</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ADC45123.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_6515"/>
    <dbReference type="EnsemblBacteria" id="ADC45123">
        <property type="protein sequence ID" value="ADC45123"/>
        <property type="gene ID" value="Rmet_6515"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6515"/>
    <dbReference type="HOGENOM" id="CLU_3047196_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="33"/>
            <end position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADC45123.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="54" mass="5801" checksum="DE0F00BEC2CA4173" modified="2010-03-23" version="1">MFDSARALSAAWIVGGMPVLGLLSYKLSSLHPILIIALAAWVFGLIPAMNKLFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-03-23" modified="2020-10-07" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>D3DXW9</accession>
    <name>D3DXW9_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_6532</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ADC45139.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADC45139">
        <property type="protein sequence ID" value="ADC45139"/>
        <property type="gene ID" value="Rmet_6532"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6532"/>
    <dbReference type="HOGENOM" id="CLU_3398084_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADC45139.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="31" mass="3623" checksum="BE562ED95EFB8E54" modified="2010-03-23" version="1">MPQLFMFGSFRMLAQTINVPRLSGHDWKADK</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-03-23" modified="2022-08-03" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>D3DY24</accession>
    <name>D3DY24_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Rmet_6593</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="5">Belongs to the Vsr family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ADC45194.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_6593"/>
    <dbReference type="EnsemblBacteria" id="ADC45194">
        <property type="protein sequence ID" value="ADC45194"/>
        <property type="gene ID" value="Rmet_6593"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6593"/>
    <dbReference type="HOGENOM" id="CLU_2452427_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006298">
        <property type="term" value="P:mismatch repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004603">
        <property type="entry name" value="DNA_mismatch_endonuc_vsr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011335">
        <property type="entry name" value="Restrct_endonuc-II-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03852">
        <property type="entry name" value="Vsr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52980">
        <property type="entry name" value="SSF52980"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0227">DNA damage</keyword>
    <keyword evidence="4" id="KW-0234">DNA repair</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0540">Nuclease</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00029466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ADC45194.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="89" mass="9939" checksum="D9B55473FEBA74F3" modified="2010-03-23" version="1">MAGDPNGKPGARITNVPAIREGHPQLMAITRSHATDSGQRYCSETARLLRLLWHKGYRLNRTDLPGRSDVAFIVQRKVIFVHGCFLARA</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-03-23" modified="2021-06-02" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>D3DY52</accession>
    <name>D3DY52_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_3753</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ADC45222.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3753"/>
    <dbReference type="EnsemblBacteria" id="ADC45222">
        <property type="protein sequence ID" value="ADC45222"/>
        <property type="gene ID" value="Rmet_3753"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3753"/>
    <dbReference type="eggNOG" id="ENOG5030X6H">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_208855_1_2_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADC45222.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="55" mass="5976" checksum="6DCE1C7833433214" modified="2010-03-23" version="1">MASKVIRSNTMAKSQLRSTREAKKPKQPKKPAVPGTPFGTTHSRVGNEGVAKRKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-03-23" modified="2020-10-07" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>D3DYA0</accession>
    <name>D3DYA0_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_6682</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ADC45270.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADC45270">
        <property type="protein sequence ID" value="ADC45270"/>
        <property type="gene ID" value="Rmet_6682"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6682"/>
    <dbReference type="HOGENOM" id="CLU_2555808_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADC45270.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="82" mass="8893" checksum="10A3F62060197BA6" modified="2010-03-23" version="1">MFADVKHLMHYKRGEGCSALAQQLHFEMLKRVLARDAAKQFGSPDRLLAGVAGAARLEPGRPIAPGVLEVFQKAAVVLYGFA</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-03-23" modified="2020-10-07" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>D3DYG2</accession>
    <name>D3DYG2_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_6753</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ADC45332.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADC45332">
        <property type="protein sequence ID" value="ADC45332"/>
        <property type="gene ID" value="Rmet_6753"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6753"/>
    <dbReference type="HOGENOM" id="CLU_2957393_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADC45332.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="59" mass="6490" checksum="712161A6E5126D27" modified="2010-03-23" version="1">MRSGPPLRGGMMSGTCHGHEPGSVLVPQKFRRAERKMPGERCPADLVGVFNPFLPFPVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-03-23" modified="2020-10-07" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>D3DYH2</accession>
    <name>D3DYH2_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_6764</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ADC45342.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_6764"/>
    <dbReference type="EnsemblBacteria" id="ADC45342">
        <property type="protein sequence ID" value="ADC45342"/>
        <property type="gene ID" value="Rmet_6764"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6764"/>
    <dbReference type="HOGENOM" id="CLU_2882724_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADC45342.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="63" mass="7540" checksum="9306F086086C5979" modified="2010-03-23" version="1">MRKTPNRSRGGQTLLTRSNRARLWTRWIQSWRAGDWEVSGNLEDLHFLEYGQPYIPRQPITIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-03-23" modified="2022-08-03" version="51" xmlns="http://uniprot.org/uniprot">
    <accession>D3DYJ7</accession>
    <name>D3DYJ7_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">DNA-binding protein Hu-beta (Bacterial histone-like DNA-binding protein)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">hupB</name>
        <name evidence="5" type="ordered locus">Rmet_6397</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="5 6">
        <name>pMOL28</name>
    </geneLocation>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="6">Plasmid pMOL28</plasmid>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the bacterial histone-like protein family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000355">
        <property type="protein sequence ID" value="ADC45367.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADC45367">
        <property type="protein sequence ID" value="ADC45367"/>
        <property type="gene ID" value="Rmet_6397"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6397"/>
    <dbReference type="HOGENOM" id="CLU_1794877_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid pMOL28"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030527">
        <property type="term" value="F:structural constituent of chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.520.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000119">
        <property type="entry name" value="Hist_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020816">
        <property type="entry name" value="Histone-like_DNA-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010992">
        <property type="entry name" value="IHF-like_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33175">
        <property type="entry name" value="PTHR33175"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00216">
        <property type="entry name" value="Bac_DNA_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01727">
        <property type="entry name" value="DNABINDINGHU"/>
    </dbReference>
    <dbReference type="SMART" id="SM00411">
        <property type="entry name" value="BHL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47729">
        <property type="entry name" value="SSF47729"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00045">
        <property type="entry name" value="HISTONE_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0614">Plasmid</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="105"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="105"/>
            <end position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003939"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ADC45367.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="144" mass="15699" checksum="B8F1A3EE39E277F1" modified="2010-03-23" version="1">MTKSELIDAVARGVDGLTKTKAEQVLNATLDAIVEAVGRGETLSLIGFGTFSKGERGERMARNPRTGEEIRVEAAKTVKFKAGQRFKDAVNGGRWPVTRCMRPPHNTCRSRSCSPAPRRSRNASMRRRSDIAASLAMSMACSMV</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="67" xmlns="http://uniprot.org/uniprot">
    <accession>Q1L9I8</accession>
    <name>Q1L9I8_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">HNH endonuclease typeIV restriction enzyme</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_6329</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3 4">
        <name>pMOL28</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid pMOL28</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000355">
        <property type="protein sequence ID" value="ABF13188.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF13188">
        <property type="protein sequence ID" value="ABF13188"/>
        <property type="gene ID" value="Rmet_6329"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6329"/>
    <dbReference type="HOGENOM" id="CLU_074569_0_0_4"/>
    <dbReference type="OMA" id="ARVRLCW"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid pMOL28"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00085">
        <property type="entry name" value="HNHc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003615">
        <property type="entry name" value="HNH_nuc"/>
    </dbReference>
    <dbReference type="SMART" id="SM00507">
        <property type="entry name" value="HNHc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0540">Nuclease</keyword>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HNHc" evidence="2">
        <location>
            <begin position="267"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="18"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="229"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="24"/>
            <end position="38"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00507"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF13188.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="348" mass="38620" checksum="5912AA3FC2CA51EF" modified="2006-05-30" version="1">MRSGAFLPVDTYPAIMGKSTMDAKTKPRQFRTQESLQAERRSRNLVKPFLESRGFVAVDDNRRPVGEAESQTVSATSPSGTRISARVRLCWRYQRGKPETISAAQLRARLTDDDWDLTLSEIRDRAMESGVTHFLLLQPRGDGFDFAALLPVADVPRIWRAQCETSESLIATGKLGRIRKNHAKNGGSPTLYLKDTRTPDAHAVPDVLWSWPGVSDLCSLQEVGGESPFGPIDDTLDDLQAPSPAAAGSDSPERRTAERSYVKRDDRVRRQVLDRADGVCENPTCSIRAPFPSFLDVHHIFGVETSDRAWNCVAICPNCHRAAHYAPDRSAINEELLRVASRADHEAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="51" xmlns="http://uniprot.org/uniprot">
    <accession>Q1L9J8</accession>
    <name>Q1L9J8_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">RES domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_6319</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3 4">
        <name>pMOL28</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid pMOL28</plasmid>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the MbcT/ParT/Res family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000355">
        <property type="protein sequence ID" value="ABF13178.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008649485.1">
        <property type="nucleotide sequence ID" value="NC_007972.2"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF13178">
        <property type="protein sequence ID" value="ABF13178"/>
        <property type="gene ID" value="Rmet_6319"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6319"/>
    <dbReference type="HOGENOM" id="CLU_102935_0_0_4"/>
    <dbReference type="OMA" id="ELHARHY"/>
    <dbReference type="OrthoDB" id="1538724at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid pMOL28"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014914">
        <property type="entry name" value="RES_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08808">
        <property type="entry name" value="RES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00953">
        <property type="entry name" value="RES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RES" evidence="2">
        <location>
            <begin position="49"/>
            <end position="187"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF13178.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="218" mass="24030" checksum="1113826145BCFA63" modified="2006-05-30" version="1">MPVLTAEALGCPVPPQDLAARVMPVKELDPGVTKLWRIHRGVYGPIHYNRRSTGGQPYRFDAPDDTFGVLYASPSFAACMAEAVVRDRFQGESLPLLLDEAELTLRCVSRLGTATNRPLRLADLTQPHFHLGMDNRILSTADYRGPNLWSAAIHAAYPDIDGLYFTSRFANEASVAIFDRVRIVAQGEPIPLAQFPLLPAFLEAYDIGIAPAQDPWNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="57" xmlns="http://uniprot.org/uniprot">
    <accession>Q1L9R0</accession>
    <name>Q1L9R0_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">NACHT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_6257</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2 3">
        <name>pMOL28</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid pMOL28</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000355">
        <property type="protein sequence ID" value="ABF13116.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011514896.1">
        <property type="nucleotide sequence ID" value="NC_007972.2"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF13116">
        <property type="protein sequence ID" value="ABF13116"/>
        <property type="gene ID" value="Rmet_6257"/>
    </dbReference>
    <dbReference type="GeneID" id="60824662"/>
    <dbReference type="KEGG" id="rme:Rmet_6257"/>
    <dbReference type="HOGENOM" id="CLU_002002_0_0_4"/>
    <dbReference type="OMA" id="LHVIYAT"/>
    <dbReference type="OrthoDB" id="7323at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid pMOL28"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007111">
        <property type="entry name" value="NACHT_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05729">
        <property type="entry name" value="NACHT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NACHT" evidence="1">
        <location>
            <begin position="271"/>
            <end position="441"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF05729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF13116.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="1474" mass="164633" checksum="696565F950F685BB" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-12-02" version="58" xmlns="http://uniprot.org/uniprot">
    <accession>Q1L9V1</accession>
    <name>Q1L9V1_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Phosphoadenosine phosphosulfate reductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_6216</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="1 2">
        <name>pMOL28</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid pMOL28</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000355">
        <property type="protein sequence ID" value="ABF13075.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF13075">
        <property type="protein sequence ID" value="ABF13075"/>
        <property type="gene ID" value="Rmet_6216"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6216"/>
    <dbReference type="HOGENOM" id="CLU_019017_0_0_4"/>
    <dbReference type="OMA" id="RTPENGV"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid pMOL28"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF13075.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="496" mass="55203" checksum="8A5D0619A04DFE1F" modified="2006-05-30" version="1">MHQYGRDHDFKVIVKVVQPSMAATFQLKVLSGRALPSFPGTHGDCSTDLKISPQRAFRRKLFRDLAAKGLPPPVTCLGTRYDESARRAAAMRSRGESDHAAMQNKDGDWVVSPIARWSDDDVWEAVALYGSGALPGFSDFEEMRRIYAHSVGTSCAVVADAILDGAARKQGRCGARLGCHVCQMAEDKSLANMIAFDERYAYARGLHRLNCFIRATRHDWERRHWIGRTIRGGYIKIQPDTYHPAMLRQLTRFMLQLDFDEERRAAAAGDAPKFRLLPVDLMIAVDAMQSLNGVARPFAAWADLRDIRARGIRYDIPDVPEVAPTPIPTARFLHVGDGWDESAPCADWTGLRDPMRESLTEGSCCAPAIVTTSDGRAVLDLPTEQQFDVDAESAAFIVDFEVERLLAMHDAGNRPGSITAGYRWYLHFGCLTLSHSQKVEHDDIARRTAFKDRLGLTDAYDVRDVLARSVPPEALPGRAREAWGNHAIKQAQLALC</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-06-17" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LA44</accession>
    <name>Q1LA44_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">CopE involved in Cu(II) resistance</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">copE</name>
        <name evidence="1" type="ordered locus">Rmet_6123</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="1 2">
        <name>pMOL30</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid pMOL30</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000354">
        <property type="protein sequence ID" value="ABF12982.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF12982">
        <property type="protein sequence ID" value="ABF12982"/>
        <property type="gene ID" value="Rmet_6123"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6123"/>
    <dbReference type="HOGENOM" id="CLU_1304046_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid pMOL30"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF12982.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="211" mass="23741" checksum="DEE425F67198C82F" modified="2006-05-30" version="1">MHRDLFRRASGTDAMPISDRRTRTFFCCFNMLMACPTPISLSPPYALIEWLRSLPSAVSRELLQDAVSPMASYMPGLLGSAGEPDVDALSSWLRSSNDELPVVTWSKVVALRAHLQCLLAERGKDAWWAHRREGLQRALDELTIGSKDGHPARLPAELQFYLASLAAERRIWADAIRQWYGLCATQLSDSALAKWVIAEDRRRFAAPRNET</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="66" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LAN3</accession>
    <name>Q1LAN3_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">BPL/LPL catalytic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_5934</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12793.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518139.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5934"/>
    <dbReference type="EnsemblBacteria" id="ABF12793">
        <property type="protein sequence ID" value="ABF12793"/>
        <property type="gene ID" value="Rmet_5934"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5934"/>
    <dbReference type="eggNOG" id="COG0095">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_084176_0_0_4"/>
    <dbReference type="OMA" id="FCDGRFN"/>
    <dbReference type="OrthoDB" id="871298at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004143">
        <property type="entry name" value="BPL_LPL_catalytic"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="SSF55681"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51733">
        <property type="entry name" value="BPL_LPL_CATALYTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BPL/LPL catalytic" evidence="1">
        <location>
            <begin position="27"/>
            <end position="227"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF12793.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="246" mass="26389" checksum="2BED8DDFBB299427" modified="2006-05-30" version="1">MTFTFVESDPTETDPLQGELALLEHAISTGPVAQLWEAPLSLVIPRSYLRQPGIEEARAQFAAQGCPVWLRMSGGGLVPQGPGILNVSLAYAVPRGVATWMEPVYLHLCEVIAQGLREVGVETHWQAVEGSFCDGRFNLAWGPGDSARKIAGTAQYWRRAPSAVQTPDGQRHLVLAHAILLVDADPVEINARANAFEAAIHSGRHYDPAKVVSVHEASGIDDATGLTETVKTSIRNAIARSTAPGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LAZ0</accession>
    <name>Q1LAZ0_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">AMP-dependent synthetase and ligase</fullName>
            <ecNumber evidence="3">6.2.1.26</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_5827</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EC" id="6.2.1.26" evidence="3"/>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12686.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518037.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5827"/>
    <dbReference type="EnsemblBacteria" id="ABF12686">
        <property type="protein sequence ID" value="ABF12686"/>
        <property type="gene ID" value="Rmet_5827"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5827"/>
    <dbReference type="eggNOG" id="COG0318">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000022_59_0_4"/>
    <dbReference type="OMA" id="FTQAYGM"/>
    <dbReference type="OrthoDB" id="377638at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008756">
        <property type="term" value="F:o-succinylbenzoate-CoA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025110">
        <property type="entry name" value="AMP-bd_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045851">
        <property type="entry name" value="AMP-bd_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020845">
        <property type="entry name" value="AMP-binding_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_Synth/Lig"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13193">
        <property type="entry name" value="AMP-binding_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00455">
        <property type="entry name" value="AMP_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0436">Ligase</keyword>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AMP-binding" evidence="1">
        <location>
            <begin position="8"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="domain" description="AMP-binding_C" evidence="2">
        <location>
            <begin position="424"/>
            <end position="499"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF12686.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="518" mass="56516" checksum="8DF4D84D9F62F843" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="92" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LBM6</accession>
    <name>Q1LBM6_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Drug resistance transporter EmrB/QacA subfamily</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Rmet_5591</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="8">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="8">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12450.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519999.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5591"/>
    <dbReference type="EnsemblBacteria" id="ABF12450">
        <property type="protein sequence ID" value="ABF12450"/>
        <property type="gene ID" value="Rmet_5591"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5591"/>
    <dbReference type="eggNOG" id="COG0477">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000960_28_0_4"/>
    <dbReference type="OMA" id="TQKIGYY"/>
    <dbReference type="OrthoDB" id="1108236at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004638">
        <property type="entry name" value="EmrB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="SSF103473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00711">
        <property type="entry name" value="efflux_EmrB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0614">Plasmid</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="18"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="58"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="83"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="143"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="171"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="204"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="238"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="276"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="312"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="342"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="493"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="domain" description="MFS" evidence="6">
        <location>
            <begin position="20"/>
            <end position="516"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABF12450.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="524" mass="55691" checksum="D143D82E795D6C4A" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="98" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LBS0</accession>
    <name>Q1LBS0_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative transcriptional regulator, LuxR family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_5547</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12406.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5547"/>
    <dbReference type="EnsemblBacteria" id="ABF12406">
        <property type="protein sequence ID" value="ABF12406"/>
        <property type="gene ID" value="Rmet_5547"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5547"/>
    <dbReference type="eggNOG" id="COG2197">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_077414_0_0_4"/>
    <dbReference type="OMA" id="MPARECE"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016032">
        <property type="entry name" value="Sig_transdc_resp-reg_C-effctor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000792">
        <property type="entry name" value="Tscrpt_reg_LuxR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039420">
        <property type="entry name" value="WalR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48111">
        <property type="entry name" value="PTHR48111"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00196">
        <property type="entry name" value="GerE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00421">
        <property type="entry name" value="HTH_LUXR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46894">
        <property type="entry name" value="SSF46894"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH luxR-type" evidence="2">
        <location>
            <begin position="205"/>
            <end position="262"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00421"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF12406.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="276" mass="30816" checksum="FD5B877E8CF6DAB5" modified="2006-05-30" version="1">MQMTRGETAKMGAGSADDTSFMADAVGAIGTETFGSQLDVLVDHYCRIDFCACYRIASDRVDVVAMSDPNRFESATRIDTYANGKLWETDPALHQAFVGLESNSLSYARLRRTDIADHRLRDVVYTHLVDRLLVCYKNKAATYAISLLRWRSSAPFLYGDIESLLSRSPVIMALVDRHFVSSVKHGRPADAFASRELAEQCLRAVSGMPARECEVCARVIFGFSLPAIASDLGISVDTVKSYVKRAYQRIEIGSQRELVVAYLEYWEGWARSRILS</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="99" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LBT6</accession>
    <name>Q1LBT6_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Putative transcriptional regulator, GntR-family (HTH)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Rmet_5531</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="7">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="7">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12390.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519935.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5531"/>
    <dbReference type="EnsemblBacteria" id="ABF12390">
        <property type="protein sequence ID" value="ABF12390"/>
        <property type="gene ID" value="Rmet_5531"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5531"/>
    <dbReference type="eggNOG" id="COG2186">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017584_9_0_4"/>
    <dbReference type="OMA" id="VTHERTA"/>
    <dbReference type="OrthoDB" id="1875458at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07377">
        <property type="entry name" value="WHTH_GntR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.530">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011711">
        <property type="entry name" value="GntR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008920">
        <property type="entry name" value="TF_FadR/GntR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000524">
        <property type="entry name" value="Tscrpt_reg_HTH_GntR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07729">
        <property type="entry name" value="FCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00392">
        <property type="entry name" value="GntR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00035">
        <property type="entry name" value="HTHGNTR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00895">
        <property type="entry name" value="FCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00345">
        <property type="entry name" value="HTH_GNTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48008">
        <property type="entry name" value="SSF48008"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50949">
        <property type="entry name" value="HTH_GNTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0614">Plasmid</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH gntR-type" evidence="5">
        <location>
            <begin position="68"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="33"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABF12390.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="303" mass="33606" checksum="48B62586EF413F52" modified="2006-05-30" version="1">MWEAMLGARNAVEETLKCRMRHTLSDNICAEVDPMQDTPKMPTKTKPAVPDQQDPSPAGPIFRRVRTRRAFEAVSDQIRQQLASGELRPGDRLPGERELAEQFTVSRSAVREALRSLEAASVVEARTGINGGFFIRSTSSTTGLTQTVQDLVSLGQVSIANVTEARVELMAVAIRLACARATEEELDAIEADISYHTELFRQGDGSRNSKSVIEFYRLIAQATHNAVIVLMVDALSEIIRKLLAQIAPEPRKDIMQVRRKVLTLLRQRDAQGACDAMATHLRHVSDYLESESKKSARKKKIAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LC58</accession>
    <name>Q1LC58_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">HlyD_D23 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_5409</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12268.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519816.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5409"/>
    <dbReference type="EnsemblBacteria" id="ABF12268">
        <property type="protein sequence ID" value="ABF12268"/>
        <property type="gene ID" value="Rmet_5409"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5409"/>
    <dbReference type="eggNOG" id="COG0845">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018816_5_0_4"/>
    <dbReference type="OMA" id="CSAYQAQ"/>
    <dbReference type="OrthoDB" id="1432684at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032317">
        <property type="entry name" value="HlyD_D23"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006143">
        <property type="entry name" value="RND_pump_MFP"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16576">
        <property type="entry name" value="HlyD_D23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01730">
        <property type="entry name" value="RND_mfp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HlyD_D23" evidence="2">
        <location>
            <begin position="67"/>
            <end position="263"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF16576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF12268.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="273" mass="28199" checksum="EE9C49DF540103A2" modified="2006-05-30" version="1">MSNASSRFSFRLSGLSGLSGLSGLAGLAVVSAALFPGVAVGANDPSPPSRAVDPGAIRVLLSADPETTMAATMNGTVQSLGVTLGQTITKGRPLVQMDCAEPRARLGMADAELAGARETLTAKSALRKLDAVGDMELNLAKSAADRASAAVALARTQVAYCTVAAPFSGRVARVYVRPHESVNAGAPLLDLVSDGPLKLRLNVPSRMLRDLRIGSQFNVNVTETGKSYTAKVSAINARVDAVEQSIEIEGRLTAPAAELLPGMSGVAQFQQTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-10-07" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LCM6</accession>
    <name>Q1LCM6_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">ATP-grasp domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_5241</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12100.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5241"/>
    <dbReference type="EnsemblBacteria" id="ABF12100">
        <property type="protein sequence ID" value="ABF12100"/>
        <property type="gene ID" value="Rmet_5241"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5241"/>
    <dbReference type="eggNOG" id="COG2232">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_052967_0_0_4"/>
    <dbReference type="OMA" id="DDSFSER"/>
    <dbReference type="OrthoDB" id="1677960at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011761">
        <property type="entry name" value="ATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013815">
        <property type="entry name" value="ATP_grasp_subdomain_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="SSF56784"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50975">
        <property type="entry name" value="ATP_GRASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ATP-grasp" evidence="2">
        <location>
            <begin position="108"/>
            <end position="289"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF12100.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="429" mass="47975" checksum="7B4A853712F85931" modified="2006-05-30" version="1">MNASMNKKLKVLVFPCGSENAAEIHQALRYSLHVELFGASSVDDHGRFRFERYVGGLPKISDAEFDSTFSRLLVELDIDVVFATHDSVLDYLAPRAPGMGVTLINGDPQTAAIARRKSATYACFRDADWAPHMFESVEAVKAWPIIVKPDLGQGGQGVTLANDPQQARAAMDAMQFPVLVEYLPGEEITVDCFTDRKRRLVWTGPRTRERVRAGITMRSRLLALSPEIEGIAREINQRLQLRGPWFFQLKADKDGRWKLLEISCRVAGTMVAQRARGVNLPLMAVQDHLGRDLVALDNPHVNLVERSIATRTRLDFAYDSVFVDLDDTLVLNGHAVPQTMAFLYQSKADGKKVNLITRHAFDVAQTLEKAHISRGIFDQIIVITDGGSKADHFSPDSIFIDNHFPERLEVARKLAIPVLDVDMVEFLIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="104" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LCS5</accession>
    <name>Q1LCS5_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 5">Kynureninase</fullName>
            <ecNumber evidence="4 5">3.7.1.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">L-kynurenine hydrolase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="4" type="primary">kynU</name>
        <name evidence="7" type="ordered locus">Rmet_5192</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="8">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="8">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4 5">Catalyzes the cleavage of L-kynurenine (L-Kyn) and L-3-hydroxykynurenine (L-3OHKyn) into anthranilic acid (AA) and 3-hydroxyanthranilic acid (3-OHAA), respectively.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>3-hydroxy-L-kynurenine + H2O = 3-hydroxyanthranilate + H(+) + L-alanine</text>
            <dbReference type="Rhea" id="RHEA:25143"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:36559"/>
            <dbReference type="ChEBI" id="CHEBI:57972"/>
            <dbReference type="ChEBI" id="CHEBI:58125"/>
            <dbReference type="EC" id="3.7.1.3"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4 5">
            <text>H2O + L-kynurenine = anthranilate + H(+) + L-alanine</text>
            <dbReference type="Rhea" id="RHEA:16813"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16567"/>
            <dbReference type="ChEBI" id="CHEBI:57959"/>
            <dbReference type="ChEBI" id="CHEBI:57972"/>
            <dbReference type="EC" id="3.7.1.3"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="4 5">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="4 5">Amino-acid degradation; L-kynurenine degradation; L-alanine and anthranilate from L-kynurenine: step 1/1.</text>
    </comment>
    <comment type="pathway">
        <text evidence="4 5">Cofactor biosynthesis; NAD(+) biosynthesis; quinolinate from L-kynurenine: step 2/3.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4 5">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 5">Belongs to the kynureninase family.</text>
    </comment>
    <dbReference type="EC" id="3.7.1.3" evidence="4 5"/>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12051.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519598.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5192"/>
    <dbReference type="EnsemblBacteria" id="ABF12051">
        <property type="protein sequence ID" value="ABF12051"/>
        <property type="gene ID" value="Rmet_5192"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5192"/>
    <dbReference type="eggNOG" id="COG3844">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003433_4_1_4"/>
    <dbReference type="OMA" id="GIICDKR"/>
    <dbReference type="OrthoDB" id="1474443at2"/>
    <dbReference type="UniPathway" id="UPA00253">
        <property type="reaction ID" value="UER00329"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00334">
        <property type="reaction ID" value="UER00455"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061981">
        <property type="term" value="F:3-hydroxykynureninase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030429">
        <property type="term" value="F:kynureninase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034354">
        <property type="term" value="P:'de novo' NAD biosynthetic process from tryptophan"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043420">
        <property type="term" value="P:anthranilate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097053">
        <property type="term" value="P:L-kynurenine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019805">
        <property type="term" value="P:quinolinate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006569">
        <property type="term" value="P:tryptophan catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01970">
        <property type="entry name" value="Kynureninase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000192">
        <property type="entry name" value="Aminotrans_V_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010111">
        <property type="entry name" value="Kynureninase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14084">
        <property type="entry name" value="PTHR14084"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00266">
        <property type="entry name" value="Aminotran_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038800">
        <property type="entry name" value="KYNU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="SSF53383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01814">
        <property type="entry name" value="kynureninase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4 5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0614">Plasmid</keyword>
    <keyword evidence="1 4 5" id="KW-0662">Pyridine nucleotide biosynthesis</keyword>
    <keyword evidence="3 4 5" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminotran_5" evidence="6">
        <location>
            <begin position="70"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="region of interest" description="Pyridoxal phosphate binding" evidence="4">
        <location>
            <begin position="125"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="97"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="98"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="165"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="194"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="197"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="219"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="249"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="275"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="4">
        <location>
            <position position="220"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRNR" id="PIRNR038800"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABF12051.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="409" mass="44655" checksum="D6AFFBC5AC4735C5" modified="2006-05-30" version="1">MMIREDCAALDAADKLAGCRARFALPAGTIYLDGNSLGAMPANVPARMKVAIEQEWAEGLIRSWNDADWYPAPQRTGDRIARLIGAGQGEVIVADSTSVNLFKVLVAATRMRPGRGVILAERTNFPTDVYIAESVAEMTGCELRCVDPEEVMSAIDENVAIVSLTHVNYKTGKRYDMEAMTRRAHEAGALVVWDLCHTAGAMPVYLNRCEVDFAVGCGYKYLNGGPGAPAFVFVASRHLEHVRQPLSGWHGHAQPFAFTHEYAPHAGIDRMLTGTAPQLGLISLESALEAFDGVDMEALREKSVSLGDLFITLAEQELAGLGFTLATPRDADQRGSQVSLRHAEGYAIMQALIARNIIGDFRAPDILRFGFAALYVRHVDIWDTIAALKDIVTSEAWNTEAFRTRKSVT</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-10-07" version="58" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LCU4</accession>
    <name>Q1LCU4_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_5173</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12032.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5173"/>
    <dbReference type="EnsemblBacteria" id="ABF12032">
        <property type="protein sequence ID" value="ABF12032"/>
        <property type="gene ID" value="Rmet_5173"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5173"/>
    <dbReference type="HOGENOM" id="CLU_173088_0_0_4"/>
    <dbReference type="OMA" id="PLIYLFD"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF12032.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="94" mass="10551" checksum="2391F98955DF6977" modified="2006-05-30" version="1">MDNYPAANCKGFDIYPLVYKFDSPREWHERRPDRSYSASVVICREGEPPSGNCARVFHVPDMHWADLGLAKRAAIRHGEHIIDGQVVGESLAGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LCZ7</accession>
    <name>Q1LCZ7_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_5120</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11979.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519526.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5120"/>
    <dbReference type="EnsemblBacteria" id="ABF11979">
        <property type="protein sequence ID" value="ABF11979"/>
        <property type="gene ID" value="Rmet_5120"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5120"/>
    <dbReference type="eggNOG" id="COG3246">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_065536_2_0_4"/>
    <dbReference type="OMA" id="AYREMTI"/>
    <dbReference type="OrthoDB" id="1892901at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019475">
        <property type="term" value="P:L-lysine catabolic process to acetate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008567">
        <property type="entry name" value="KCE_family"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37418">
        <property type="entry name" value="PTHR37418"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05853">
        <property type="entry name" value="BKACE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="334"/>
            <end position="353"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF11979.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="353" mass="39300" checksum="4D43FAD81FE8A7D9" modified="2006-05-30" version="1">MDFIDGSMFPENQQKLVITAAPYGPEWIPSDFPEDIPVTMEQQIQKAVDCYNAGATVLHVHVREADGKGSKRLSKFNELLAGIRARVPDMILQVGGSISFAPESEGDVAKWLSDDTRHMLAELKPTPDQVTIAINTNQMNVVEQMCAADVAGTSLAEPANYRAYREMTIPAGPEWVEEHIRRLSINGIQTHFQLANIAQLETVERMMRRGSCNVPLILTWVAIGGGFEAPNLYNLANFVRACPNGSVLTLETSMLNVLPLNMMAIAMGLHVRCGIEDNLWTQRRDRKMGTVEQIEQLVRISREFGRDVANAKEAREIYKIGTFYDSANETLAENGFAPNRKPGQVGFTQHARR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-10-07" version="62" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LDA7</accession>
    <name>Q1LDA7_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Rmet_5007</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="5">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="5">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11869.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5007"/>
    <dbReference type="EnsemblBacteria" id="ABF11869">
        <property type="protein sequence ID" value="ABF11869"/>
        <property type="gene ID" value="Rmet_5007"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5007"/>
    <dbReference type="eggNOG" id="ENOG503027G">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_121782_0_0_4"/>
    <dbReference type="OMA" id="EAQYKPS"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0614">Plasmid</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193079" evidence="3">
        <location>
            <begin position="21"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="73"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="30"/>
            <end position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF11869.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="191" mass="20416" checksum="904F32AE23908471" modified="2006-05-30" version="1">MSKWLSAVVCSMLVATPAGMALAQVPAGSKADHEAAIKQADADYKAASARCDGMKDNAKDVCKLEARRDRDVARADADARHEGTEKAHAKAMKTKADRDYDVAKEMCDDRKGNEKDVCVKDAKAAHERALGTAKIEKAAATGSPEDVAQARRAAQKDNLDAAYEARKERCDAMSGDARSQCQADVAAKFGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-10-07" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LDB3</accession>
    <name>Q1LDB3_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_5001</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11863.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5001"/>
    <dbReference type="EnsemblBacteria" id="ABF11863">
        <property type="protein sequence ID" value="ABF11863"/>
        <property type="gene ID" value="Rmet_5001"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5001"/>
    <dbReference type="HOGENOM" id="CLU_3398087_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF11863.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="31" mass="3438" checksum="C3F67CA540B0648F" modified="2006-05-30" version="1">MTHALHYLLKILENMNLLDAEWADPGQGEGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="62" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LDM8</accession>
    <name>Q1LDM8_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_4886</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11748.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519307.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4886"/>
    <dbReference type="EnsemblBacteria" id="ABF11748">
        <property type="protein sequence ID" value="ABF11748"/>
        <property type="gene ID" value="Rmet_4886"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4886"/>
    <dbReference type="eggNOG" id="COG4645">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041000_0_0_4"/>
    <dbReference type="OMA" id="LEIFCLG"/>
    <dbReference type="OrthoDB" id="717806at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014550">
        <property type="entry name" value="UCP028704_OpgC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38592">
        <property type="entry name" value="PTHR38592"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10129">
        <property type="entry name" value="OpgC_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF028704">
        <property type="entry name" value="UPC028704"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="15"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="47"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="86"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="146"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="168"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="200"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="229"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="276"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="315"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="344"/>
            <end position="365"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF11748.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="383" mass="41955" checksum="ADF71DA1EB2D94C3" modified="2006-05-30" version="1">MNAVNNNQRRTEVDLIRGIALLFIVVDHVNGSVLADYTIRNFTLFDAAEVFVFLAGYSAGAAYLAMEARQGASAASKRLHRRSWEIYKAFVLTGVLMLLFGFLLAMCKVPAPAVRATEAPAFMAQPIETLFQVIGLARQPFVSDVLPMYAVFIGLAPLAIGWARKYPLAFVCGSVALWLMAPALLPLLPSSEPRGWSFNPFAWQLMFGMGLLARLRPDFVLPQQNIARWACTIGAVVVTMACMALTYLWMRPHVYEAWLPAWLPSMVFPLPKQSLAFLRVLSFLGLAWLVYLAARAGWVERVARFMGPVVQIGRHSLFCFVLGAVVSVTIEGFTYGIAGGRPTRPVALGGDLVAVAILLVVGGWYGRRKEAKKVAAKSAIVTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="101" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LDQ8</accession>
    <name>Q1LDQ8_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Aldehyde dehydrogenase (Acceptor)</fullName>
            <ecNumber evidence="7">1.2.99.3</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">puuC</name>
        <name evidence="7" type="ordered locus">Rmet_4856</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="8">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="8">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the aldehyde dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.2.99.3" evidence="7"/>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11718.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519281.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4856"/>
    <dbReference type="EnsemblBacteria" id="ABF11718">
        <property type="protein sequence ID" value="ABF11718"/>
        <property type="gene ID" value="Rmet_4856"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4856"/>
    <dbReference type="eggNOG" id="COG1012">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005391_0_1_4"/>
    <dbReference type="OMA" id="ANVEHEM"/>
    <dbReference type="OrthoDB" id="744602at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016620">
        <property type="term" value="F:oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.309.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.605.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016161">
        <property type="entry name" value="Ald_DH/histidinol_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016163">
        <property type="entry name" value="Ald_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016160">
        <property type="entry name" value="Ald_DH_CS_CYS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029510">
        <property type="entry name" value="Ald_DH_CS_GLU"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016162">
        <property type="entry name" value="Ald_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015590">
        <property type="entry name" value="Aldehyde_DH_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00171">
        <property type="entry name" value="Aldedh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53720">
        <property type="entry name" value="SSF53720"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00070">
        <property type="entry name" value="ALDEHYDE_DEHYDR_CYS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00687">
        <property type="entry name" value="ALDEHYDE_DEHYDR_GLU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 4 7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-0614">Plasmid</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aldedh" evidence="6">
        <location>
            <begin position="28"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="active site" evidence="2">
        <location>
            <position position="255"/>
        </location>
    </feature>
    <feature type="active site" evidence="3">
        <location>
            <position position="289"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABF11718.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="483" mass="50933" checksum="180C9DA7FF13C658" modified="2006-05-30" version="1">MTATRPDLPKHDLLIDGKRLPPGSGEYSTDINPATEEPIALVAQGSAADVDTAVLAARAALKSWAGMRAADRGRILNRFADLLEAHAEELITLESLDAGKPLAAVRRQDLPAVVDTVRYYAGWCDKIHGAVIPTRPDALTYTVREPVGVVAAIIPWNFPLMIGMWKIAPALACGCTLIVKPAEITPLTALRVAELALEAGVPPGVFNVVTGKGSVVGNALVAHPGIDKVTFTGSPGVGRGIMQGAASNFKRVTLELGGKSANVIFADADIDAATRAAASGIFFNSGQVCSAGSRILAQRKVYDEVVERLTTRARAIRVGDPASPDTTMGPVVSAAQMKTVLDYVEIGKREGASAVTGGARIGQRGFFVEPTVFANVEHEMRISQEEIFGPVASVIPFEDEAEAVRIANGTAFSLAAGVWSADIARVHKVAGELRAGTVWINTYGYTDVRLPWGGAGDSGLGREHGDAAIENFTEPKAIWLSLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LDR8</accession>
    <name>Q1LDR8_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative TRAP-type C4-dicarboxylate transport system, periplasmic component</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_4846</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11708.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519271.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4846"/>
    <dbReference type="EnsemblBacteria" id="ABF11708">
        <property type="protein sequence ID" value="ABF11708"/>
        <property type="gene ID" value="Rmet_4846"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4846"/>
    <dbReference type="eggNOG" id="COG1638">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036176_2_3_4"/>
    <dbReference type="OMA" id="WPASGIW"/>
    <dbReference type="OrthoDB" id="752834at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071702">
        <property type="term" value="P:organic substance transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018389">
        <property type="entry name" value="DctP_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038404">
        <property type="entry name" value="TRAP_DctP_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33376">
        <property type="entry name" value="PTHR33376"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03480">
        <property type="entry name" value="DctP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193388" evidence="2">
        <location>
            <begin position="40"/>
            <end position="351"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF11708.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="351" mass="37085" checksum="9DF69F7DF3514AED" modified="2006-05-30" version="1">MHPLRHRLALATLTLATATGYTASALVALIAPFAPNAHAQPAPVVWQMATEYPATSMPGEGLTTFAQEVATRTQGTLAPQPTFDASAGVKSAQMPAAIRDGKLQAGDAFGGALGEVDPIFLLSSLPFVATSVDKAHRLADLARADYAQAFEKQGQHLLYITPWPPTGLWTHKPVGTLDDLRTLSVRAYDETSADVMRAAGAKAQNLSFADAMPRLKDGSVNAVLSSGDGGAGRKLWDYLPYFTAIGYAMPLSFTTVNAAAWQSLTPAQRDAVTAAARATEGKQWSRLNTRLTENTARMRANGVTIDDNPAPAVQQALQAAGLSAVGVWQTRVGERGAALLKTYFSAEAGLH</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-12-02" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LDV8</accession>
    <name>Q1LDV8_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative DNA-binding transcriptional regulator, Helix-turn-helix motif</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_4806</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11668.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4806"/>
    <dbReference type="EnsemblBacteria" id="ABF11668">
        <property type="protein sequence ID" value="ABF11668"/>
        <property type="gene ID" value="Rmet_4806"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4806"/>
    <dbReference type="eggNOG" id="COG1396">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_085376_5_0_4"/>
    <dbReference type="OMA" id="AGRFEWY"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00093">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001387">
        <property type="entry name" value="Cro/C1-type_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013096">
        <property type="entry name" value="Cupin_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010982">
        <property type="entry name" value="Lambda_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011051">
        <property type="entry name" value="RmlC_Cupin_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07883">
        <property type="entry name" value="Cupin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01381">
        <property type="entry name" value="HTH_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00530">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47413">
        <property type="entry name" value="SSF47413"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51182">
        <property type="entry name" value="SSF51182"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50943">
        <property type="entry name" value="HTH_CROC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH cro/C1-type" evidence="2">
        <location>
            <begin position="25"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF11668.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="203" mass="21212" checksum="DC57AEA9844A40E5" modified="2006-05-30" version="1">MTMSQPSPSKTPMTAEGPPAVGSALQALRQSQQLSLDELSRRAGVSKSMLSQIERNLANPTVAVLWRLANALGVSLTDFLAQGTAAPQAPAITVVPPHATPALKSPDTRCELRILGPIDMAGRFEWYELSIQPGGVLASEPHEAGTQEHLSVLTGSMTVRSGGDEKKLKHGDTARYGADVAHAIGNPGKSVATALLVVVHPGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-10-07" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LDW2</accession>
    <name>Q1LDW2_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_4802</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11664.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4802"/>
    <dbReference type="EnsemblBacteria" id="ABF11664">
        <property type="protein sequence ID" value="ABF11664"/>
        <property type="gene ID" value="Rmet_4802"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4802"/>
    <dbReference type="HOGENOM" id="CLU_2481058_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="67"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF11664.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="87" mass="9885" checksum="0AC2EDA728E5BE19" modified="2006-05-30" version="1">MRPWNAGWHCRHTPDRPGPARRAFPGRAGNSGHRHPDLSFPARHFAFGRHPWRRNSSCILEKAMTPIYRKTICAIVAVAALYVLINS</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LE87</accession>
    <name>Q1LE87_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 7">Muconolactone Delta-isomerase</fullName>
            <shortName evidence="7">MIase</shortName>
            <ecNumber evidence="5 7">5.3.3.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">catC2</name>
        <name evidence="9" type="ordered locus">Rmet_4677</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="10">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="10">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 7">
            <text>(S)-muconolactone = (4,5-dihydro-5-oxofuran-2-yl)-acetate</text>
            <dbReference type="Rhea" id="RHEA:12348"/>
            <dbReference type="ChEBI" id="CHEBI:58425"/>
            <dbReference type="ChEBI" id="CHEBI:58736"/>
            <dbReference type="EC" id="5.3.3.4"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 7">Aromatic compound metabolism; beta-ketoadipate pathway; 5-oxo-4,5-dihydro-2-furylacetate from catechol: step 3/3.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homodecamer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 7">Belongs to the muconolactone Delta-isomerase family.</text>
    </comment>
    <dbReference type="EC" id="5.3.3.4" evidence="5 7"/>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11539.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008646082.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4677"/>
    <dbReference type="EnsemblBacteria" id="ABF11539">
        <property type="protein sequence ID" value="ABF11539"/>
        <property type="gene ID" value="Rmet_4677"/>
    </dbReference>
    <dbReference type="GeneID" id="60821711"/>
    <dbReference type="KEGG" id="rme:Rmet_4677"/>
    <dbReference type="eggNOG" id="COG4829">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_080702_2_0_4"/>
    <dbReference type="OMA" id="MCRHPSS"/>
    <dbReference type="OrthoDB" id="1857318at2"/>
    <dbReference type="UniPathway" id="UPA00157">
        <property type="reaction ID" value="UER00260"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016159">
        <property type="term" value="F:muconolactone delta-isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042952">
        <property type="term" value="P:beta-ketoadipate pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011008">
        <property type="entry name" value="Dimeric_a/b-barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026029">
        <property type="entry name" value="MLI_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003464">
        <property type="entry name" value="Muconolactone_d_Isoase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02426">
        <property type="entry name" value="MIase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001486">
        <property type="entry name" value="CatC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54909">
        <property type="entry name" value="SSF54909"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03221">
        <property type="entry name" value="muco_delta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0058">Aromatic hydrocarbons catabolism</keyword>
    <keyword evidence="7 9" id="KW-0413">Isomerase</keyword>
    <keyword evidence="9" id="KW-0614">Plasmid</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MIase" evidence="8">
        <location>
            <begin position="1"/>
            <end position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001739"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011365"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022797"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR001486"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02426"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ABF11539.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="94" mass="10674" checksum="AB44D6FE50D261B8" modified="2006-05-30" version="1">MLFMVEMTVRIPQSLDPEVADAIKAKEKAYSQQLQRDGKWRHLWRVAGLYANVSIFDVKDADELHTLLTGLPLYPYMEMKVTALAQHGSAIAQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LEP7</accession>
    <name>Q1LEP7_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Tyrosine-based site-specific recombinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">int</name>
        <name evidence="7" type="ordered locus">Rmet_4514</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
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        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="8">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
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    <dbReference type="EnsemblBacteria" id="ABF11379">
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        <property type="gene ID" value="Rmet_4514"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4514"/>
    <dbReference type="eggNOG" id="COG0582">
        <property type="taxonomic scope" value="Bacteria"/>
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    <dbReference type="HOGENOM" id="CLU_027562_42_1_4"/>
    <dbReference type="OMA" id="PTPHERW"/>
    <dbReference type="OrthoDB" id="745068at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="CB"/>
    </dbReference>
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        <property type="entry name" value="DNA_brk_join_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022169">
        <property type="entry name" value="DUF3701"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013762">
        <property type="entry name" value="Integrase-like_cat_sf"/>
    </dbReference>
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        <property type="entry name" value="Integrase_catalytic"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
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            <end position="320"/>
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        <source>
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    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
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    </evidence>
    <evidence type="ECO:0000256" key="4">
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51900"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="ABF11379.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="559" mass="61909" checksum="CC388DB40F46B129" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="77" xmlns="http://uniprot.org/uniprot">
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        <submittedName>
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        <name evidence="2" type="ordered locus">Rmet_4211</name>
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    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
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            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
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    <dbReference type="EnsemblBacteria" id="ABF11078">
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        <property type="gene ID" value="Rmet_4211"/>
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    <dbReference type="KEGG" id="rme:Rmet_4211"/>
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    <dbReference type="OrthoDB" id="895090at2"/>
    <dbReference type="Proteomes" id="UP000002429">
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    <proteinExistence type="predicted"/>
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    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="339"/>
            <end position="359"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
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    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF11078.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="390" mass="42012" checksum="85585D7FD9F4D1FF" modified="2006-05-30" version="1">MQHLEALTREAAALARGALPLTADEGEVARTLQICNACRYCEGFCAVFPAMTRRLEFPKADIHYLANLCHNCGACYHACQYAPPHEFAVNIPQAMAKVRVQTYGDYAFPAALGGLYRRAGLTTALALAGGLALFLLMVLAMRGSLLHAPLAGNFYAIFPHNLLAVMFGVVFVFAIVALGIGVTRFWRQVTPGTRASDDGGAAAGGAAHDALRLKYLGGGHGEGCNNEDDAFTLLRRRFHHFTFYGFMLCFAATTVATLYHYLLDLHAPYALTSLPVLLGTVGGIGLLIGTAGLFWLNVRRDPRTGDPDQKPMDRGFIALLFLTSASGLVLLALRDTGAMATSLAVHLGIVMALFLTLPYGKFAHGIYRCAALLKYNIEKRRPSDLALGAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="105" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LFQ5</accession>
    <name>Q1LFQ5_CUPMC</name>
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        <submittedName>
            <fullName evidence="4">Transposase ISRme8</fullName>
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    <gene>
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        <name evidence="3" type="ordered locus">Rmet_2879</name>
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    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
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        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid</name>
    </geneLocation>
    <geneLocation type="plasmid" evidence="5">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="submission" date="2006-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of the chromosome of Ralstonia metallidurans CH34.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Copeland A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Barry K."/>
                <person name="Detter J.C."/>
                <person name="Glavina del Rio T."/>
                <person name="Hammon N."/>
                <person name="Israni S."/>
                <person name="Dalin E."/>
                <person name="Tice H."/>
                <person name="Martinez M."/>
                <person name="Goltsman E."/>
                <person name="Pitluck S."/>
                <person name="Schmutz J."/>
                <person name="Larimer F."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Kim E."/>
                <person name="Mergeay M."/>
                <person name="Benotmane M.A."/>
                <person name="Vallaeys T."/>
                <person name="Michaux A."/>
                <person name="Monchy S."/>
                <person name="Dunn J."/>
                <person name="McCorkle S."/>
                <person name="Taghavi S."/>
                <person name="van der Lelie D."/>
                <person name="Richardson P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">CH34</strain>
            <plasmid evidence="4">megaplasmid</plasmid>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
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                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
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                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
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            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="5">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2010-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Benotmane R."/>
                <person name="Lapidus A."/>
                <person name="McCorkle S."/>
                <person name="Monchy S."/>
                <person name="Taghavi S."/>
                <person name="van der Lelie N."/>
                <person name="Dunn J."/>
                <person name="Leys N."/>
                <person name="Mergeay M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">CH34</strain>
            <plasmid evidence="4">megaplasmid</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09752.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11021.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2879"/>
    <dbReference type="EnsemblBacteria" id="ABF09752">
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        <property type="gene ID" value="Rmet_2879"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF11021">
        <property type="protein sequence ID" value="ABF11021"/>
        <property type="gene ID" value="Rmet_4154"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2879"/>
    <dbReference type="KEGG" id="rme:Rmet_4154"/>
    <dbReference type="eggNOG" id="COG3385">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028400_1_0_4"/>
    <dbReference type="OMA" id="WHINEHR"/>
    <dbReference type="OrthoDB" id="1254910at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
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        <property type="term" value="F:transposase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006313">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002559">
        <property type="entry name" value="Transposase_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024473">
        <property type="entry name" value="Transposases_IS4_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01609">
        <property type="entry name" value="DDE_Tnp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Nterm_IS4"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SSF53098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0614">Plasmid</keyword>
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        <location>
            <begin position="22"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="domain" description="DDE_Tnp_1" evidence="1">
        <location>
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            <end position="360"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF09752.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF11021.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="402" mass="44011" checksum="0AD16980C66AB3AB" modified="2006-05-30" version="1">MRVMARTRKTLPSRVDVAHLISAGVLASVCPRTLIEEVLAETGKASQRERLLPAPAVVYYVMALALWREAPLEEVLRVVCEGLQWLGGGHTEAVQASKSAISQARSRLGPEVMRQLADRVLRPLAAPGAPGAWYRGLRVMALDGSCMDVADEAANAKFFGYPGASRGQSAFPQARVLGLVECGTHAVVAAGIAPYGHSEQVMAAQLLPAKLTPEMLVLADRNFYGFKLWQTACATGAKLAWRVKSNLKLPVEQMLPDGSYLSRVFDSDDRARRAGQTVRVIDYALEGSATPAQGSYRLLTNLLDPDAAPALELAALYHERWEIEGVFDEFKTHLRANSTVLRSKTPELVQQELWGLLLAHFAIRQLMAQAAWARELDPDRLSFMHAVRVIKRKMPQAAAVSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LFS5</accession>
    <name>Q1LFS5_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Pyruvate dehydrogenase E1 component subunit alpha</fullName>
            <ecNumber evidence="3">1.2.4.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3 5" type="primary">pdhA</name>
        <name evidence="5" type="ordered locus">Rmet_4134</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="6">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="6">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>(R)-N(6)-lipoyl-L-lysyl-[dihydrolipoyllysine-residue acetyltransferase] + H(+) + pyruvate = (R)-N(6)-(S(8)-acetyldihydrolipoyl)-L-lysyl-[dihydrolipoyllysine-residue acetyltransferase] + CO2</text>
            <dbReference type="Rhea" id="RHEA:19189"/>
            <dbReference type="Rhea" id="RHEA-COMP:10480"/>
            <dbReference type="Rhea" id="RHEA-COMP:10481"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:83099"/>
            <dbReference type="ChEBI" id="CHEBI:83111"/>
            <dbReference type="EC" id="1.2.4.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 3">
            <name>thiamine diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58937"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="3">Heterodimer of an alpha and a beta chain.</text>
    </comment>
    <dbReference type="EC" id="1.2.4.1" evidence="3"/>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11001.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008650901.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4134"/>
    <dbReference type="EnsemblBacteria" id="ABF11001">
        <property type="protein sequence ID" value="ABF11001"/>
        <property type="gene ID" value="Rmet_4134"/>
    </dbReference>
    <dbReference type="GeneID" id="60823282"/>
    <dbReference type="KEGG" id="rme:Rmet_4134"/>
    <dbReference type="eggNOG" id="COG1071">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029393_1_0_4"/>
    <dbReference type="OMA" id="LGYEMPC"/>
    <dbReference type="OrthoDB" id="1248201at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004739">
        <property type="term" value="F:pyruvate dehydrogenase (acetyl-transferring) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006096">
        <property type="term" value="P:glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001017">
        <property type="entry name" value="DH_E1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017596">
        <property type="entry name" value="PdhA/BkdA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11516:SF41">
        <property type="entry name" value="PTHR11516:SF41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00676">
        <property type="entry name" value="E1_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="SSF52518"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03181">
        <property type="entry name" value="PDH_E1_alph_x"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="2 3 5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0786">Thiamine pyrophosphate</keyword>
    <feature type="domain" description="E1_dh" evidence="4">
        <location>
            <begin position="39"/>
            <end position="319"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU366007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABF11001.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="367" mass="39385" checksum="85FEA62A4360FF0D" modified="2006-05-30" version="1">MGTVARFEIGFTRYLSPDGSPEPDLPDFARDPATLLPLYRAMVLTRQFDLKAIAMQRTGQIGTFASALGQEAIGVGVATAMRRDDVLVPSYRDHAAQFVRGVTMTESLLYWGGDERGSAFAAVPYDFGNCVPIGTQVGHAAGVAYTFKLRQAPNVAVCILGDGGTSKGDFYEGMNMAGAWHVPLVIVINNNQWAISMPRAKQTAAATLAQKAIAAGIPGEQIDGNDVIAVRQRVGAAIEHARHGGGPTLIEAVSYRLGDHTTADDASRYRDEASVKEAWRCEPIIRLRDYLARLGAWDAAQEEALIKECQQAVGAAVQAYLALPHPDASAMFDCLYETLPDAMAEQLDTARQYDASGKTDTSGAHHG</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="95" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LFZ6</accession>
    <name>Q1LFZ6_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 10">Periplasmic nitrate reductase, electron transfer subunit</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9 10">Diheme cytochrome c NapB</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="primary">napB</name>
        <name evidence="13" type="ordered locus">Rmet_4062</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="14">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="14">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Electron transfer subunit of the periplasmic nitrate reductase complex NapAB.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Component of the periplasmic nitrate reductase NapAB complex composed of NapA and NapB.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Periplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="PTM">
        <text evidence="11">Binds 2 heme C groups per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the NapB family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10930.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518557.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4062"/>
    <dbReference type="EnsemblBacteria" id="ABF10930">
        <property type="protein sequence ID" value="ABF10930"/>
        <property type="gene ID" value="Rmet_4062"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4062"/>
    <dbReference type="eggNOG" id="COG3043">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_103367_2_0_4"/>
    <dbReference type="OMA" id="KCMSCHA"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042597">
        <property type="term" value="C:periplasmic space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009061">
        <property type="term" value="P:anaerobic respiration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036280">
        <property type="entry name" value="Multihaem_cyt_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005591">
        <property type="entry name" value="NapB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38604">
        <property type="entry name" value="PTHR38604"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03892">
        <property type="entry name" value="NapB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006105">
        <property type="entry name" value="NapB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48695">
        <property type="entry name" value="SSF48695"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0249">Electron transport</keyword>
    <keyword evidence="5 11" id="KW-0349">Heme</keyword>
    <keyword evidence="8 12" id="KW-0408">Iron</keyword>
    <keyword evidence="6 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0574">Periplasm</keyword>
    <keyword evidence="13" id="KW-0614">Plasmid</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0813">Transport</keyword>
    <feature type="binding site" description="axial binding residue" evidence="12">
        <location>
            <position position="74"/>
        </location>
        <ligand>
            <name>heme c</name>
            <dbReference type="ChEBI" id="CHEBI:61717"/>
            <label>1</label>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <feature type="binding site" description="covalent" evidence="11">
        <location>
            <position position="88"/>
        </location>
        <ligand>
            <name>heme c</name>
            <dbReference type="ChEBI" id="CHEBI:61717"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" description="covalent" evidence="11">
        <location>
            <position position="91"/>
        </location>
        <ligand>
            <name>heme c</name>
            <dbReference type="ChEBI" id="CHEBI:61717"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="12">
        <location>
            <position position="92"/>
        </location>
        <ligand>
            <name>heme c</name>
            <dbReference type="ChEBI" id="CHEBI:61717"/>
            <label>1</label>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="12">
        <location>
            <position position="109"/>
        </location>
        <ligand>
            <name>heme c</name>
            <dbReference type="ChEBI" id="CHEBI:61717"/>
            <label>2</label>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <feature type="binding site" description="covalent" evidence="11">
        <location>
            <position position="128"/>
        </location>
        <ligand>
            <name>heme c</name>
            <dbReference type="ChEBI" id="CHEBI:61717"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" description="covalent" evidence="11">
        <location>
            <position position="131"/>
        </location>
        <ligand>
            <name>heme c</name>
            <dbReference type="ChEBI" id="CHEBI:61717"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="12">
        <location>
            <position position="132"/>
        </location>
        <ligand>
            <name>heme c</name>
            <dbReference type="ChEBI" id="CHEBI:61717"/>
            <label>2</label>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007368"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013773"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00031832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRNR" id="PIRNR006105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR006105-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR006105-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ABF10930.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="163" mass="18431" checksum="3BEE935D518CCC27" modified="2006-05-30" version="1">MLPSRSWVPLPLLVLMLVVMLLSVPLARAQGLVDSMRGPTPVANETSPPILFPTENKDIRRTRNYTMQPPTIPHKIDSYQLDKDFNRCMFCHARTRTEETQAIPVSITHYMDRDNNVLAEISPRRYFCTQCHVPQADAKPLVNNTFVDVEAILKRSGGTKGAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-10-07" version="54" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LG09</accession>
    <name>Q1LG09_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_4049</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10917.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4049"/>
    <dbReference type="EnsemblBacteria" id="ABF10917">
        <property type="protein sequence ID" value="ABF10917"/>
        <property type="gene ID" value="Rmet_4049"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4049"/>
    <dbReference type="eggNOG" id="COG1285">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2772948_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF10917.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="69" mass="7135" checksum="9218789EB1A2AE8F" modified="2006-05-30" version="1">MKPRRVSGSAVPVPQSRDLEVRAFGDQDVEIEAALVATSADGDALDASVARLGGTSVVSQAFWSASTTE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-12-12" modified="2022-05-25" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LG90</accession>
    <name>CHEB2_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Protein-glutamate methylesterase/protein-glutamine glutaminase 2</fullName>
            <ecNumber evidence="1">3.1.1.61</ecNumber>
            <ecNumber evidence="1">3.5.1.44</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">cheB2</name>
        <name type="ordered locus">Rmet_3968</name>
    </gene>
    <organism>
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in chemotaxis. Part of a chemotaxis signal transduction system that modulates chemotaxis in response to various stimuli. Catalyzes the demethylation of specific methylglutamate residues introduced into the chemoreceptors (methyl-accepting chemotaxis proteins or MCP) by CheR. Also mediates the irreversible deamidation of specific glutamine residues to glutamic acid.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>[protein]-L-glutamate 5-O-methyl ester + H2O = H(+) + L-glutamyl-[protein] + methanol</text>
            <dbReference type="Rhea" id="RHEA:23236"/>
            <dbReference type="Rhea" id="RHEA-COMP:10208"/>
            <dbReference type="Rhea" id="RHEA-COMP:10311"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17790"/>
            <dbReference type="ChEBI" id="CHEBI:29973"/>
            <dbReference type="ChEBI" id="CHEBI:82795"/>
            <dbReference type="EC" id="3.1.1.61"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>H2O + L-glutaminyl-[protein] = L-glutamyl-[protein] + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:16441"/>
            <dbReference type="Rhea" id="RHEA-COMP:10207"/>
            <dbReference type="Rhea" id="RHEA-COMP:10208"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:29973"/>
            <dbReference type="ChEBI" id="CHEBI:30011"/>
            <dbReference type="EC" id="3.5.1.44"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="1">Contains a C-terminal catalytic domain, and an N-terminal region which modulates catalytic activity.</text>
    </comment>
    <comment type="PTM">
        <text evidence="1">Phosphorylated by CheA. Phosphorylation of the N-terminal regulatory domain activates the methylesterase activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the CheB family.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.61" evidence="1"/>
    <dbReference type="EC" id="3.5.1.44" evidence="1"/>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10836.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518475.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q1LG90"/>
    <dbReference type="SMR" id="Q1LG90"/>
    <dbReference type="STRING" id="266264.Rmet_3968"/>
    <dbReference type="EnsemblBacteria" id="ABF10836">
        <property type="protein sequence ID" value="ABF10836"/>
        <property type="gene ID" value="Rmet_3968"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3968"/>
    <dbReference type="eggNOG" id="COG2201">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_51_0_4"/>
    <dbReference type="OMA" id="QHINAAF"/>
    <dbReference type="OrthoDB" id="1655418at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000156">
        <property type="term" value="F:phosphorelay response regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008984">
        <property type="term" value="F:protein-glutamate methylesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050568">
        <property type="term" value="F:protein-glutamine glutaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006935">
        <property type="term" value="P:chemotaxis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018277">
        <property type="term" value="P:protein deamination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006482">
        <property type="term" value="P:protein demethylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd16432">
        <property type="entry name" value="CheB_Rec"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.180">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00099">
        <property type="entry name" value="CheB_chemtxs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008248">
        <property type="entry name" value="CheB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035909">
        <property type="entry name" value="CheB_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000673">
        <property type="entry name" value="Sig_transdc_resp-reg_Me-estase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01339">
        <property type="entry name" value="CheB_methylest"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000876">
        <property type="entry name" value="RR_chemtxs_CheB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52738">
        <property type="entry name" value="SSF52738"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50122">
        <property type="entry name" value="CHEB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0145">Chemotaxis</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0597">Phosphoprotein</keyword>
    <keyword id="KW-0614">Plasmid</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000264301" description="Protein-glutamate methylesterase/protein-glutamine glutaminase 2">
        <location>
            <begin position="1"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="domain" description="Response regulatory" evidence="1">
        <location>
            <begin position="2"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="domain" description="CheB-type methylesterase" evidence="1">
        <location>
            <begin position="145"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="158"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="185"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="278"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="1">
        <location>
            <position position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00099"/>
        </source>
    </evidence>
    <sequence length="338" mass="35253" checksum="B21509FF38E60151" modified="2006-05-30" version="1">MRIGIVNDSALAVAALRRALALDTTLEIAWIAGDGEEAVRMAATQTPDLILMDLLMPVMDGVEATRRIMAESPCAIVVVTMDLGRNANQVFDAMGHGAIDAVDTPTLTDSDTKLAAGPLLRKIRNIARLLGGRGQAPHPLAAATPTPTAPRLVAIGASAGGPAALATLLGALPADFGAAVVAVQHVDEAFAQGMAEWLDAQCQLPVRLARAGEVPQAGAVVLAGTNDHLRLTSAGRLIYTPDPCDYLYRPSIDVFFESVVEHWRGEAIGVLLTGMGRDGAQGLKAMRERGFQTIAQDQATSAVYGMPKAAATLGAASEILPLQKIAPRLVMTCGGGRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="100" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LGB9</accession>
    <name>Q1LGB9_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Two component transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Rmet_3939</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="6">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="6">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10807.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3939"/>
    <dbReference type="EnsemblBacteria" id="ABF10807">
        <property type="protein sequence ID" value="ABF10807"/>
        <property type="gene ID" value="Rmet_3939"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3939"/>
    <dbReference type="eggNOG" id="COG2197">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_90_1_4"/>
    <dbReference type="OMA" id="QTGWEVC"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06170">
        <property type="entry name" value="LuxR_C_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016032">
        <property type="entry name" value="Sig_transdc_resp-reg_C-effctor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000792">
        <property type="entry name" value="Tscrpt_reg_LuxR_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00196">
        <property type="entry name" value="GerE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00421">
        <property type="entry name" value="HTH_LUXR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46894">
        <property type="entry name" value="SSF46894"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50043">
        <property type="entry name" value="HTH_LUXR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="5" id="KW-0614">Plasmid</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Response regulatory" evidence="4">
        <location>
            <begin position="83"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="domain" description="HTH luxR-type" evidence="3">
        <location>
            <begin position="229"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="2">
        <location>
            <position position="135"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABF10807.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="300" mass="33608" checksum="7BF6F58AAFA2B9CC" modified="2006-05-30" version="1">MSPTQGNAIRFLRILLLPSRDPGINLAVHRGALRVVSRLIRGSACAQLLRVRAVRHSRVRRFFDASVQPYPTHMFRSHWMTLRILLADDHPVITAAIRARVQSSQTGWEVCGEATSATELQQQVDLLQPDVVVTDYHMPGPTELDGVRLITSLRRRHPSISVVVLTMITNPLVLRTILDVRVHGLLIKDSPLAEIMTAIGRAQRGFTYIGKSALRALAPTELRTMLPCGRDGEQRPSAREMEVLRLYLTGRTVTEIATTLCRSVKTISRQKNCAMQKLGVTNDRELFECVALQGMVLPRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="104" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LGJ9</accession>
    <name>Q1LGJ9_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative sulfurtransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_3859</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10727.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518374.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3859"/>
    <dbReference type="EnsemblBacteria" id="ABF10727">
        <property type="protein sequence ID" value="ABF10727"/>
        <property type="gene ID" value="Rmet_3859"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3859"/>
    <dbReference type="eggNOG" id="COG2603">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_043456_0_0_4"/>
    <dbReference type="OMA" id="RPLVYCW"/>
    <dbReference type="OrthoDB" id="1052499at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043828">
        <property type="term" value="F:tRNA 2-selenouridine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002098">
        <property type="term" value="P:tRNA wobble uridine modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.250.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001763">
        <property type="entry name" value="Rhodanese-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036873">
        <property type="entry name" value="Rhodanese-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017582">
        <property type="entry name" value="SelU"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00581">
        <property type="entry name" value="Rhodanese"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00450">
        <property type="entry name" value="RHOD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52821">
        <property type="entry name" value="SSF52821"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03167">
        <property type="entry name" value="tRNA_sel_U_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50206">
        <property type="entry name" value="RHODANESE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0711">Selenium</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Rhodanese" evidence="2">
        <location>
            <begin position="15"/>
            <end position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF10727.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="353" mass="39022" checksum="9CF888AD4A619042" modified="2006-05-30" version="1">MKPVVRVEDRHAFDTIIDARSPAEFEIDHIPGAINCPVLDNEERRIVGTLYKQVGAFEARRVGGAMVAANLARHLREQFADRPSGWRPLVYCWRGGLRSGSMTTWMRMVGWDAQQLAGGYKAWRRHVIDVLDTQAARAPLVVICGATGSGKTRVLHALAAQGEQVLDLEGLARHKGSLLGALPDVAQPSQTAFETALADVIERVDLARPLYVEGEGARIGKLNLPVPLVAHMRESACVEIDASPETRLAFLLHDYAYLGDRPAWLADQLGRLKELHGKTIIAAWQEMATTGNLRQLFGELATEHYDPSYARSQRKHFHKWDARRVVHADSLSAQGIEALARSIVQTVAEQQPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LGN2</accession>
    <name>Q1LGN2_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative ferredoxin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_3824</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10694.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518340.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3824"/>
    <dbReference type="EnsemblBacteria" id="ABF10694">
        <property type="protein sequence ID" value="ABF10694"/>
        <property type="gene ID" value="Rmet_3824"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3824"/>
    <dbReference type="eggNOG" id="COG0633">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_082632_7_2_4"/>
    <dbReference type="OMA" id="RIEWPGL"/>
    <dbReference type="OrthoDB" id="1885467at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00207">
        <property type="entry name" value="fer2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036010">
        <property type="entry name" value="2Fe-2S_ferredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001041">
        <property type="entry name" value="2Fe-2S_ferredoxin-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012675">
        <property type="entry name" value="Beta-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00111">
        <property type="entry name" value="Fer2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54292">
        <property type="entry name" value="SSF54292"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51085">
        <property type="entry name" value="2FE2S_FER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="2Fe-2S ferredoxin-type" evidence="1">
        <location>
            <begin position="13"/>
            <end position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF10694.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="102" mass="10741" checksum="2D5C40B58A7CB793" modified="2006-05-30" version="1">MTGDGNTFDDTEYTACVLPANTRFAAPASLSLLEAALLEGVSLPSSCRNGTCRACISKLHAGSVRYRVEWPGLSLDEKEDGYILPCVACPTSDVVFEPVGAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="101" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LGZ1</accession>
    <name>Q1LGZ1_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Transcriptional regulator, LysR family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_3713</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the LysR transcriptional regulatory family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10585.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518233.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF10585">
        <property type="protein sequence ID" value="ABF10585"/>
        <property type="gene ID" value="Rmet_3713"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3713"/>
    <dbReference type="eggNOG" id="COG0583">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039613_39_0_4"/>
    <dbReference type="OMA" id="CLFRRGH"/>
    <dbReference type="OrthoDB" id="1591366at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005119">
        <property type="entry name" value="LysR_subst-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000847">
        <property type="entry name" value="Tscrpt_reg_HTH_LysR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00126">
        <property type="entry name" value="HTH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03466">
        <property type="entry name" value="LysR_substrate"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00039">
        <property type="entry name" value="HTHLYSR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50931">
        <property type="entry name" value="HTH_LYSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH lysR-type" evidence="2">
        <location>
            <begin position="6"/>
            <end position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF10585.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="320" mass="34943" checksum="97E4860DFC6B5ABD" modified="2006-05-30" version="1">MELQDIDLNLLVVFNELLRQRKMSAVARTLGISQPAVSNALNRLRKLLDDDLFLRTSKGMIPTQLAGTLAGPITEALDVIHHSLNARATFDPLTSTRAFTVAMTDIGEIYLLPGLMERLGKLAPGVKISTVCIHGDSLKGAMESGCIDLAIGFLPGLTAGFFQRRLLSQQYVCIFRKGHPLGRRDMTLEAFAMADRVSISAEGTGHTRVDAAIQQAGVTRRSRLSVPHFAALGHILQSTDLIAVVPQAYAERALVLFNLVSAPCPVKISEISVNVFWHATRHREVGNQWLRNVISDRFAVCPIPEATMSPQGLVVNCRGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LGZ9</accession>
    <name>Q1LGZ9_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">4-oxalomesaconate hydratase</fullName>
            <ecNumber evidence="4">4.2.1.83</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">ligJ</name>
        <name evidence="4" type="ordered locus">Rmet_3705</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="5">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="5">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EC" id="4.2.1.83" evidence="4"/>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10577.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518225.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3705"/>
    <dbReference type="EnsemblBacteria" id="ABF10577">
        <property type="protein sequence ID" value="ABF10577"/>
        <property type="gene ID" value="Rmet_3705"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3705"/>
    <dbReference type="eggNOG" id="COG2159">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_813586_0_0_4"/>
    <dbReference type="OMA" id="IHGHYTT"/>
    <dbReference type="OrthoDB" id="1832218at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047584">
        <property type="term" value="F:4-oxalmesaconate hydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016831">
        <property type="term" value="F:carboxy-lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032465">
        <property type="entry name" value="ACMSD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006680">
        <property type="entry name" value="Amidohydro-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21240">
        <property type="entry name" value="PTHR21240"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04909">
        <property type="entry name" value="Amidohydro_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="SSF51556"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 4" id="KW-0456">Lyase</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0614">Plasmid</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Amidohydro-rel" evidence="3">
        <location>
            <begin position="3"/>
            <end position="329"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF04909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF10577.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="341" mass="38399" checksum="CEA1007A4BE54ECE" modified="2006-05-30" version="1">MIIDCHGHFTTAPKALEDWRQRQIASLNIPALAPSVSELRISDDELRDAIASNQLRLMRERGMDLTIFSPRASFMAHHIGSFETSATWAAICNTLCHRVSELFPDQFVGAAMLPQSPGIDTKSCVKELERCVLEYGFVGVNLNPDPSGGHWTSPPLSDPYWYPIYEKLVELDVPAMIHVSTSCNACFHTTGAHYLNADTTAFMQVLTSDLFRRFPTLKFVIPHGGGAVPYHWGRFRGLAQEMKLPPLGEHLLNNVFFDTCVYHQPGIDLLTRVIPVKNVLFASEMIGAVRGIDPETGHYYDDTRRYIDQAALSDEERHQIYEGNARRVYSRLDARLKARGH</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="65" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LH52</accession>
    <name>Q1LH52_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative tight adherence (TadE/G) protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_3652</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10524.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518179.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3652"/>
    <dbReference type="EnsemblBacteria" id="ABF10524">
        <property type="protein sequence ID" value="ABF10524"/>
        <property type="gene ID" value="Rmet_3652"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3652"/>
    <dbReference type="eggNOG" id="COG4961">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_129220_0_0_4"/>
    <dbReference type="OMA" id="VARIEFK"/>
    <dbReference type="OrthoDB" id="2068547at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012495">
        <property type="entry name" value="TadE"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07811">
        <property type="entry name" value="TadE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="37"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF10524.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="153" mass="15709" checksum="6C68A6BF5158278B" modified="2006-05-30" version="1">MHLRGKADGRRAKGIAALEFAIVAPLFLTLVLGITYYGTVFVLQQALTLAAEEGARAALRYPLTSTGGTTASTLDLRVAAAAQTARAMLPTSMSSLISSANVAQPVTCASPSGTQCVQVTLSLPTRSILPSVPMVPVPAVLTGSAMVQLSPDI</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-10-17" modified="2022-05-25" version="66" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LH90</accession>
    <name>YIDD_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Putative membrane protein insertion efficiency factor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">Rmet_3614</name>
    </gene>
    <organism>
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Could be involved in insertion of integral membrane proteins into the membrane.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell inner membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0161 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10486.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q1LH90"/>
    <dbReference type="STRING" id="266264.Rmet_3614"/>
    <dbReference type="EnsemblBacteria" id="ABF10486">
        <property type="protein sequence ID" value="ABF10486"/>
        <property type="gene ID" value="Rmet_3614"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3614"/>
    <dbReference type="eggNOG" id="COG0759">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_144811_2_2_4"/>
    <dbReference type="OMA" id="QPTCSHY"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00386">
        <property type="entry name" value="UPF0161_YidD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002696">
        <property type="entry name" value="Membr_insert_effic_factor"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33383">
        <property type="entry name" value="PTHR33383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01809">
        <property type="entry name" value="YidD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01234">
        <property type="entry name" value="Haemolytic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00278">
        <property type="entry name" value="TIGR00278"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0997">Cell inner membrane</keyword>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000253150" description="Putative membrane protein insertion efficiency factor">
        <location>
            <begin position="1"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="71"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="81"/>
            <end position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00386"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="96" mass="10342" checksum="9ECFDB2BAA1809CE" modified="2006-05-30" version="1">MKRILLALLRIYKIALSPYLGSQCRFLPTCSDYARDAIVQHGAARGTWMAACRLCRCHPFTKGGYDPVPATHGTAAAPPASAAPGRPPVTVRLPRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="97" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LHC8</accession>
    <name>Q1LHC8_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Two component transcriptional regulator, LuxR family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Rmet_3576</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10448.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517990.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3576"/>
    <dbReference type="EnsemblBacteria" id="ABF10448">
        <property type="protein sequence ID" value="ABF10448"/>
        <property type="gene ID" value="Rmet_3576"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3576"/>
    <dbReference type="eggNOG" id="COG2197">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_90_1_4"/>
    <dbReference type="OMA" id="HPMWRDG"/>
    <dbReference type="OrthoDB" id="1687028at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06170">
        <property type="entry name" value="LuxR_C_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016032">
        <property type="entry name" value="Sig_transdc_resp-reg_C-effctor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000792">
        <property type="entry name" value="Tscrpt_reg_LuxR_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00196">
        <property type="entry name" value="GerE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00038">
        <property type="entry name" value="HTHLUXR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00421">
        <property type="entry name" value="HTH_LUXR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46894">
        <property type="entry name" value="SSF46894"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00622">
        <property type="entry name" value="HTH_LUXR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50043">
        <property type="entry name" value="HTH_LUXR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Response regulatory" evidence="4">
        <location>
            <begin position="18"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="domain" description="HTH luxR-type" evidence="3">
        <location>
            <begin position="156"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="2">
        <location>
            <position position="73"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABF10448.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="228" mass="24218" checksum="CCC28EACE817EF83" modified="2006-05-30" version="1">MTAPHDADPAVTTSSAARVLLIDDHALVRDGMRMHLTLQPGLEVVGEADDGEAALAWLAAAEAGRMPDLVITDIGMRGMGGIALAGALHDRYPELAVLIVSMHDNLEYVRQAIRAGARGYVLKDAPADELLAAIQAVLAGRVFYSARIARGMAAQAVSPLDALTPRERDILHGIGRGQANKDIAVQLGVSVRTVETHRLNLKRKLGIEGRAELVKYAVQQLGLDVEGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="95" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LHQ9</accession>
    <name>Q1LHQ9_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Transcriptional regulator, GntR-family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Rmet_3445</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10317.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008646146.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3445"/>
    <dbReference type="EnsemblBacteria" id="ABF10317">
        <property type="protein sequence ID" value="ABF10317"/>
        <property type="gene ID" value="Rmet_3445"/>
    </dbReference>
    <dbReference type="GeneID" id="60822550"/>
    <dbReference type="KEGG" id="rme:Rmet_3445"/>
    <dbReference type="eggNOG" id="COG1802">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017584_5_0_4"/>
    <dbReference type="OMA" id="VIALYGQ"/>
    <dbReference type="OrthoDB" id="1911275at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07377">
        <property type="entry name" value="WHTH_GntR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.530">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011711">
        <property type="entry name" value="GntR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008920">
        <property type="entry name" value="TF_FadR/GntR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000524">
        <property type="entry name" value="Tscrpt_reg_HTH_GntR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07729">
        <property type="entry name" value="FCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00392">
        <property type="entry name" value="GntR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00895">
        <property type="entry name" value="FCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00345">
        <property type="entry name" value="HTH_GNTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48008">
        <property type="entry name" value="SSF48008"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50949">
        <property type="entry name" value="HTH_GNTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH gntR-type" evidence="4">
        <location>
            <begin position="8"/>
            <end position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABF10317.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="230" mass="25128" checksum="96CA5AC83983E537" modified="2006-05-30" version="1">MPEHIDPDMTAEAIADDIVAAIVSHRLPPGTKLREEALASVYRVSRTKVRAALLMLSKDKVIQIVPDKGAFVAKPSAEEAREVFTVRRILEAALAREFVAKATPADYKRIEKHLTAERKSLGGNDAQTRTRLLGDFHIVLAEVVGNSVMTGIMRELSMRSAVITMLYQSRRDATCSSDEHRAFIDAARAGDAELAVKLMVEHLAHVEAALHFEEVPAAARGKDLVAALLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="56" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LHR9</accession>
    <name>Q1LHR9_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_3435</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10307.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008646132.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3435"/>
    <dbReference type="EnsemblBacteria" id="ABF10307">
        <property type="protein sequence ID" value="ABF10307"/>
        <property type="gene ID" value="Rmet_3435"/>
    </dbReference>
    <dbReference type="GeneID" id="60822540"/>
    <dbReference type="KEGG" id="rme:Rmet_3435"/>
    <dbReference type="HOGENOM" id="CLU_2651866_0_0_4"/>
    <dbReference type="OMA" id="DIVTHYH"/>
    <dbReference type="OrthoDB" id="2017676at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="44"/>
            <end position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF10307.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="76" mass="8449" checksum="C9AB50CDD84DA51C" modified="2006-05-30" version="1">MRFHPDLHLKDLSGRLHWPHRPGAHTDIVTHYHSVTEDAEVAQATIYVSAVTAVVLLVVLAGISFGQEVMHWLGVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="69" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LHV9</accession>
    <name>Q1LHV9_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">HTH OST-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_3395</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10267.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517830.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3395"/>
    <dbReference type="EnsemblBacteria" id="ABF10267">
        <property type="protein sequence ID" value="ABF10267"/>
        <property type="gene ID" value="Rmet_3395"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3395"/>
    <dbReference type="eggNOG" id="COG1432">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_034061_4_0_4"/>
    <dbReference type="OMA" id="ETRNGGN"/>
    <dbReference type="OrthoDB" id="906667at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004540">
        <property type="term" value="F:ribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.610">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041966">
        <property type="entry name" value="LOTUS-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021139">
        <property type="entry name" value="NYN_MARF1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025605">
        <property type="entry name" value="OST-HTH/LOTUS_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01936">
        <property type="entry name" value="NYN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12872">
        <property type="entry name" value="OST-HTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51644">
        <property type="entry name" value="HTH_OST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH OST-type" evidence="2">
        <location>
            <begin position="174"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="151"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="253"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="393"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="260"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="298"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="349"/>
            <end position="363"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51644"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF10267.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="432" mass="46558" checksum="345A47D01FE25AC2" modified="2006-05-30" version="1">MTNTPRQGATLAVLIDADNAAPAIVEGLLAEVAKYGVAAVKRIYGDWTRPNLSGWKDRLLAHSIQPIQQFRYTVGKNATDSAMIIDAMDLLYTGRFDGFCIVSSDSDFTRLASRIREQGLTVYGFGERKTPKPFVTACDKFIYSDVLRADADSDSDSETGSAPARKRTTQELRQDSRLVRLLQSASQAVSDEDGWSTLGGIGNYIAKQAPEFDSRNYGYGKLSELVTATNLFEVETRNGGNNKTLWVRLKKRNKGEGGGQPQPAQPQQQQQPQQQGQSSQPSQSQQPSQQAQPQQQRPAPAPARPVQPAPEPVAVIPATEPARVTEEEDDDAAGSASTLIQEAVEVSTERPKAGGKRGKRPDVTLSDTPWPIDQSVFSAPGATVLSAQVDVPEETKAAAPAEEAKKPAKKKPAAKKAAPRKRAPAKKAAAAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LHW3</accession>
    <name>Q1LHW3_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Type II secretion system protein N</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">General secretion pathway protein N</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">gspN</name>
        <name evidence="11" type="ordered locus">Rmet_3391</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the GSP N family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10263.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517826.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3391"/>
    <dbReference type="EnsemblBacteria" id="ABF10263">
        <property type="protein sequence ID" value="ABF10263"/>
        <property type="gene ID" value="Rmet_3391"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3391"/>
    <dbReference type="eggNOG" id="ENOG502Z8UV">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_080660_0_0_4"/>
    <dbReference type="OMA" id="PMGSYRL"/>
    <dbReference type="OrthoDB" id="1844026at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015627">
        <property type="term" value="C:type II protein secretion system complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015628">
        <property type="term" value="P:protein secretion by the type II secretion system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022792">
        <property type="entry name" value="T2SS_protein-GspN"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01203">
        <property type="entry name" value="T2SSN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0653">Protein transport</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="25"/>
            <end position="47"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007208"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00021563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00030772"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ABF10263.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="268" mass="28862" checksum="40A85CA25ABD2965" modified="2006-05-30" version="1">MARLEALRLPRQRIRASRGWRRVRWTLLCIATVGVTVLVSMPATWLAERIAGETQRRVLLADAAGTIWHGSATLALSAGTGSQTATVLPGRLTWDVAFWPLLTGTARVTVSHSEAMSTPTTVAVTPSGWTVTPGSIRLPASLLEGVGAPFNTLRPDGMMRIDWSALQGKFAGNQKYGHLSMQLEQMSAAVSRLRPLGSYRAEVELTGADGTLQLKTTAGPLYLEGSGTIGRQARFDGTARTDPEAATQLAGLMSLLGRHENNVTRLRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="92" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LHY9</accession>
    <name>Q1LHY9_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Transposase catalytic site ISRme4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">tnpA</name>
        <name evidence="6" type="ordered locus">Rmet_0483</name>
        <name evidence="7" type="ordered locus">Rmet_3365</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="submission" date="2006-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of the chromosome of Ralstonia metallidurans CH34.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Copeland A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Barry K."/>
                <person name="Detter J.C."/>
                <person name="Glavina del Rio T."/>
                <person name="Hammon N."/>
                <person name="Israni S."/>
                <person name="Dalin E."/>
                <person name="Tice H."/>
                <person name="Martinez M."/>
                <person name="Goltsman E."/>
                <person name="Pitluck S."/>
                <person name="Schmutz J."/>
                <person name="Larimer F."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Kim E."/>
                <person name="Mergeay M."/>
                <person name="Benotmane M.A."/>
                <person name="Vallaeys T."/>
                <person name="Michaux A."/>
                <person name="Monchy S."/>
                <person name="Dunn J."/>
                <person name="McCorkle S."/>
                <person name="Taghavi S."/>
                <person name="van der Lelie D."/>
                <person name="Richardson P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">CH34</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2010-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Benotmane R."/>
                <person name="Lapidus A."/>
                <person name="McCorkle S."/>
                <person name="Monchy S."/>
                <person name="Taghavi S."/>
                <person name="van der Lelie N."/>
                <person name="Dunn J."/>
                <person name="Leys N."/>
                <person name="Mergeay M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07369.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10237.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515355.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0483"/>
    <dbReference type="EnsemblBacteria" id="ABF07369">
        <property type="protein sequence ID" value="ABF07369"/>
        <property type="gene ID" value="Rmet_0483"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF10237">
        <property type="protein sequence ID" value="ABF10237"/>
        <property type="gene ID" value="Rmet_3365"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0483"/>
    <dbReference type="KEGG" id="rme:Rmet_3365"/>
    <dbReference type="eggNOG" id="COG4584">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020626_2_0_4"/>
    <dbReference type="OMA" id="IRHEVNQ"/>
    <dbReference type="OrthoDB" id="362657at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032196">
        <property type="term" value="P:transposition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017894">
        <property type="entry name" value="HTH_IS21_transposase_type"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50531">
        <property type="entry name" value="HTH_IS21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0814">Transposable element</keyword>
    <keyword evidence="2" id="KW-0815">Transposition</keyword>
    <feature type="domain" description="HTH IS21-type" evidence="5">
        <location>
            <begin position="6"/>
            <end position="68"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022578"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50531"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABF07369.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABF10237.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="508" mass="58107" checksum="ADBE4F994100DC84" modified="2006-05-30" version="1">MIDVAILSIIRRWHLRDQIPLREIARRLGISRNTVRRYLRTDVTAPAYPTRQSPSKLDGHAAKLAAWLKTEANKPRKQRRTLKQIHADLCALGFTGSYDRVAAFARRWRQEQQEQARSSGRGTYIPLRFAEGEAFQFDWSEDWAVIAGERTKLQVAQFKLSHSRAFFLRAYLLQTHEMLFDAHHHAFVAWGGIPRRGIYDNMKTAVDRVRQGKLRDVNLRFGTMVSHYLFDAEFCNPASGWEKGQIEKNVQDSRHRIWQRVPAFGSLAALNDWLADQCVRLWQQTRHPELDMTIWEAWSLERPHLMPVGQAFDGFVEHTKRVSPTCLVNFERNRYSVPASFANRPISLRVYATRLLFVAEGQVIAEHVRHINRGHHPGHTVYDWRHYLAVLQRKPGALRNGAPFAELPEGFRRLQATLLKRPGGDREMVEILALVLLHDEQAVLTAVELALEAGAASKQTVLNILSRLLEGGPVAPITTPQALALQVEPQANVARYDSLRDREVPHAA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-10-03" modified="2022-05-25" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LI44</accession>
    <name>RL16_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">50S ribosomal protein L16</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rplP</name>
        <name type="ordered locus">Rmet_3310</name>
    </gene>
    <organism>
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Binds 23S rRNA and is also seen to make contacts with the A and possibly P site tRNAs.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the 50S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL16 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10182.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_006576240.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q1LI44"/>
    <dbReference type="SMR" id="Q1LI44"/>
    <dbReference type="STRING" id="266264.Rmet_3310"/>
    <dbReference type="EnsemblBacteria" id="ABF10182">
        <property type="protein sequence ID" value="ABF10182"/>
        <property type="gene ID" value="Rmet_3310"/>
    </dbReference>
    <dbReference type="GeneID" id="60826607"/>
    <dbReference type="KEGG" id="rme:Rmet_3310"/>
    <dbReference type="eggNOG" id="COG0197">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_078858_2_1_4"/>
    <dbReference type="OMA" id="KGAVEYW"/>
    <dbReference type="OrthoDB" id="1786618at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01433">
        <property type="entry name" value="Ribosomal_L16_L10e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1170.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01342">
        <property type="entry name" value="Ribosomal_L16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016180">
        <property type="entry name" value="Ribosomal_L10e/L16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036920">
        <property type="entry name" value="Ribosomal_L10e/L16_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000114">
        <property type="entry name" value="Ribosomal_L16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020798">
        <property type="entry name" value="Ribosomal_L16_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12220">
        <property type="entry name" value="PTHR12220"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00252">
        <property type="entry name" value="Ribosomal_L16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00060">
        <property type="entry name" value="RIBOSOMALL16"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54686">
        <property type="entry name" value="SSF54686"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01164">
        <property type="entry name" value="rplP_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00586">
        <property type="entry name" value="RIBOSOMAL_L16_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <keyword id="KW-0820">tRNA-binding</keyword>
    <feature type="chain" id="PRO_0000251657" description="50S ribosomal protein L16">
        <location>
            <begin position="1"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="138" mass="15604" checksum="0FC2937F831CF02A" modified="2006-05-30" version="1">MLQPKRRKYRKEQKGRNTGKATRGNAVSFGEFGLKAMGRGRLTARQIESARRAMTRHIKRGGRIWIRIFPDKPISKKPAEVRMGNGKGNPEYYVAEIQPGKMLYEMDGVSEELAREAFRLAAAKLPIATNFVVRQVGT</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-12-12" modified="2022-08-03" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LIF3</accession>
    <name>RF1_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Peptide chain release factor 1</fullName>
            <shortName evidence="1">RF-1</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">prfA</name>
        <name type="ordered locus">Rmet_3201</name>
    </gene>
    <organism>
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Peptide chain release factor 1 directs the termination of translation in response to the peptide chain termination codons UAG and UAA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="PTM">
        <text evidence="1">Methylated by PrmC. Methylation increases the termination efficiency of RF1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the prokaryotic/mitochondrial release factor family.</text>
    </comment>
    <comment type="sequence caution" evidence="2">
        <conflict type="erroneous initiation">
            <sequence resource="EMBL-CDS" id="ABF10073" version="1"/>
        </conflict>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10073.1"/>
        <property type="status" value="ALT_INIT"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_029308602.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q1LIF3"/>
    <dbReference type="SMR" id="Q1LIF3"/>
    <dbReference type="STRING" id="266264.Rmet_3201"/>
    <dbReference type="PRIDE" id="Q1LIF3"/>
    <dbReference type="EnsemblBacteria" id="ABF10073">
        <property type="protein sequence ID" value="ABF10073"/>
        <property type="gene ID" value="Rmet_3201"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3201"/>
    <dbReference type="eggNOG" id="COG0216">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036856_0_1_4"/>
    <dbReference type="OrthoDB" id="928964at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016149">
        <property type="term" value="F:translation release factor activity, codon specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00093">
        <property type="entry name" value="Rel_fac_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005139">
        <property type="entry name" value="PCRF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000352">
        <property type="entry name" value="Pep_chain_release_fac_I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045853">
        <property type="entry name" value="Pep_chain_release_fac_I_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004373">
        <property type="entry name" value="RF-1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03462">
        <property type="entry name" value="PCRF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00472">
        <property type="entry name" value="RF-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00937">
        <property type="entry name" value="PCRF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75620">
        <property type="entry name" value="SSF75620"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00019">
        <property type="entry name" value="prfA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00745">
        <property type="entry name" value="RF_PROK_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0488">Methylation</keyword>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000263328" description="Peptide chain release factor 1">
        <location>
            <begin position="1"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="modified residue" description="N5-methylglutamine" evidence="1">
        <location>
            <position position="235"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00093"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="360" mass="40131" checksum="75AF63471AC5448F" modified="2006-12-12" version="2">MKASISTKLDQLAERLDEVNALLAREDATANMDQYRKLSREHAELSPVAEQYGQYRQAQDDLATAQALLDDPEMKEFAADEIDAARERLQSLENSLQTLLLPKDPNDDRNLLLEIRAGTGGEESALFAADLLRMYTRYAERRRWQVEIMSESPSDLGGYKEVIVRIAGDAAFSRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPEADEVGEVEINPSDLRVDTFRASGAGGQHVNKTDSAVRLTHLPTGIVVECQDDRSQHRNKEKAMKVLAARIKDMQLRAAQAKEANTRRNLIGSGDRSDRIRTYNFPQGRVTDHRINLTLYKIDMIMDGDLDELISALSAEHQADQLAALGEES</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LII6</accession>
    <name>Q1LII6_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Uroporphyrinogen-III C-methyltransferase</fullName>
            <ecNumber evidence="1">2.1.1.107</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">nirE</name>
        <name evidence="9" type="ordered locus">Rmet_3168</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="6">Porphyrin-containing compound metabolism; siroheme biosynthesis; precorrin-2 from uroporphyrinogen III: step 1/1.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.107" evidence="1"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10040.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3168"/>
    <dbReference type="EnsemblBacteria" id="ABF10040">
        <property type="protein sequence ID" value="ABF10040"/>
        <property type="gene ID" value="Rmet_3168"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3168"/>
    <dbReference type="eggNOG" id="COG0007">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011276_7_0_4"/>
    <dbReference type="OMA" id="TRPEQEE"/>
    <dbReference type="UniPathway" id="UPA00262">
        <property type="reaction ID" value="UER00211"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004851">
        <property type="term" value="F:uroporphyrin-III C-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019354">
        <property type="term" value="P:siroheme biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd11642">
        <property type="entry name" value="SUMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.950.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1010.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000878">
        <property type="entry name" value="4pyrrol_Mease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035996">
        <property type="entry name" value="4pyrrol_Methylase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014777">
        <property type="entry name" value="4pyrrole_Mease_sub1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014776">
        <property type="entry name" value="4pyrrole_Mease_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006366">
        <property type="entry name" value="CobA/CysG_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00590">
        <property type="entry name" value="TP_methylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53790">
        <property type="entry name" value="SSF53790"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01469">
        <property type="entry name" value="cobA_cysG_Cterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 9" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="5" id="KW-0627">Porphyrin biosynthesis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="3 9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="TP_methylase" evidence="8">
        <location>
            <begin position="6"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="253"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="261"/>
            <end position="285"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00025705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00590"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ABF10040.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="285" mass="30228" checksum="6C3657636515748A" modified="2006-05-30" version="1">MQTTGKVWLVGVGPGDPELATMKAVRALREADVWLVDDLVPEAMLCYANPGTQIISVGKRGGRCSVSQSSIHEQALEHAQAGRCVARVKGGDPLLFGRGGEEAEFLRGHGIEVEIVNGISSGMAAAQALGIALTHRQHCHGVTFVTAHTRDDGDPDWAALVGSRTTLVIYMGMSRLAAIRDALLQAGMRSEMPVAVVMHASRGDEKRWVGSLATLTEAADAGLASPAVILVGEVVDIPNPASLTDQHAFGDLAAGLPSKQPEHDLQRERQRAHQEDHITEGTHPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LIK1</accession>
    <name>Q1LIK1_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">ABC-type branched-chain amino acid transport system, periplasmic component</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Rmet_3153</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the leucine-binding protein family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10025.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517636.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3153"/>
    <dbReference type="EnsemblBacteria" id="ABF10025">
        <property type="protein sequence ID" value="ABF10025"/>
        <property type="gene ID" value="Rmet_3153"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3153"/>
    <dbReference type="eggNOG" id="COG0683">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027128_7_0_4"/>
    <dbReference type="OMA" id="FVEMTII"/>
    <dbReference type="OrthoDB" id="776281at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006865">
        <property type="term" value="P:amino acid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028081">
        <property type="entry name" value="Leu-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000709">
        <property type="entry name" value="Leu_Ile_Val-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028082">
        <property type="entry name" value="Peripla_BP_I"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13458">
        <property type="entry name" value="Peripla_BP_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00337">
        <property type="entry name" value="LEUILEVALBP"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53822">
        <property type="entry name" value="SSF53822"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0029">Amino-acid transport</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193494" evidence="3">
        <location>
            <begin position="33"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="domain" description="Peripla_BP_6" evidence="4">
        <location>
            <begin position="47"/>
            <end position="383"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010062"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF13458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABF10025.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="398" mass="42885" checksum="D9D0888419F7857B" modified="2006-05-30" version="1">MVLQSRVNRRTFAGLLMACAIGSCGVTGAAFAANTTAGETGVTADSILLGQSAALSGPTAVLGKQMNAGARLYFDYINKQGGVFGRKIELKALDDMYEPEEAVKNTKKLIEEDRVFALFGYVGTPTSQAVMPLVNQARIPFFAPFTGAQSLRDPQSRYVFHVRAGYEEETAAIVRQIRTTGLKRIVVVFNDDAYGRAGLSGVEHAIKALPADSGVQLVAQESVIRNTVEIGDAMQTAMKAKPDAVVLISAYRTVGAFVKEALRRGYSGQFYNVSFVGTQALAKEVGPQGSGVIISQVMPHPGNATLPVVREYLRLLQAAGKQNDFDYASIEGFIAAKSFVEGLRRAGKDLTRERLVTALETMRNVDLGGYIVQFSPESHVGSKFVEMTIINSRGVVIR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-01-15" modified="2022-08-03" version="105" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LIM6</accession>
    <name>MURG_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">UDP-N-acetylglucosamine--N-acetylmuramyl-(pentapeptide) pyrophosphoryl-undecaprenol N-acetylglucosamine transferase</fullName>
            <ecNumber evidence="1">2.4.1.227</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Undecaprenyl-PP-MurNAc-pentapeptide-UDPGlcNAc GlcNAc transferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">murG</name>
        <name type="ordered locus">Rmet_3128</name>
    </gene>
    <organism>
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Cell wall formation. Catalyzes the transfer of a GlcNAc subunit on undecaprenyl-pyrophosphoryl-MurNAc-pentapeptide (lipid intermediate I) to form undecaprenyl-pyrophosphoryl-MurNAc-(pentapeptide)GlcNAc (lipid intermediate II).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>di-trans-octa-cis-undecaprenyl diphospho-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamyl-meso-2,6-diaminopimeloyl-D-alanyl-D-alanine + UDP-N-acetyl-alpha-D-glucosamine = di-trans-octa-cis-undecaprenyl diphospho-[N-acetyl-alpha-D-glucosaminyl-(1-&gt;4)]-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamyl-meso-2,6-diaminopimeloyl-D-alanyl-D-alanine + H(+) + UDP</text>
            <dbReference type="Rhea" id="RHEA:31227"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57705"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:61387"/>
            <dbReference type="ChEBI" id="CHEBI:61388"/>
            <dbReference type="EC" id="2.4.1.227"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cell wall biogenesis; peptidoglycan biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell inner membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the glycosyltransferase 28 family. MurG subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.227" evidence="1"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10000.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517620.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q1LIM6"/>
    <dbReference type="SMR" id="Q1LIM6"/>
    <dbReference type="STRING" id="266264.Rmet_3128"/>
    <dbReference type="CAZy" id="GT28">
        <property type="family name" value="Glycosyltransferase Family 28"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF10000">
        <property type="protein sequence ID" value="ABF10000"/>
        <property type="gene ID" value="Rmet_3128"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3128"/>
    <dbReference type="eggNOG" id="COG0707">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037404_2_0_4"/>
    <dbReference type="OMA" id="AADMMLC"/>
    <dbReference type="OrthoDB" id="1165736at2"/>
    <dbReference type="UniPathway" id="UPA00219"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051991">
        <property type="term" value="F:UDP-N-acetyl-D-glucosamine:N-acetylmuramoyl-L-alanyl-D-glutamyl-meso-2,6-diaminopimelyl-D-alanyl-D-alanine-diphosphoundecaprenol 4-beta-N-acetylglucosaminlytransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050511">
        <property type="term" value="F:undecaprenyldiphospho-muramoylpentapeptide beta-N-acetylglucosaminyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007049">
        <property type="term" value="P:cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030259">
        <property type="term" value="P:lipid glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009252">
        <property type="term" value="P:peptidoglycan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008360">
        <property type="term" value="P:regulation of cell shape"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00033">
        <property type="entry name" value="MurG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006009">
        <property type="entry name" value="GlcNAc_MurG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007235">
        <property type="entry name" value="Glyco_trans_28_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004276">
        <property type="entry name" value="GlycoTrans_28_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04101">
        <property type="entry name" value="Glyco_tran_28_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03033">
        <property type="entry name" value="Glyco_transf_28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01133">
        <property type="entry name" value="murG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0131">Cell cycle</keyword>
    <keyword id="KW-0132">Cell division</keyword>
    <keyword id="KW-0997">Cell inner membrane</keyword>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0133">Cell shape</keyword>
    <keyword id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword id="KW-0328">Glycosyltransferase</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-0573">Peptidoglycan synthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000315145" description="UDP-N-acetylglucosamine--N-acetylmuramyl-(pentapeptide) pyrophosphoryl-undecaprenol N-acetylglucosamine transferase">
        <location>
            <begin position="1"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="13"/>
            <end position="15"/>
        </location>
        <ligand>
            <name>UDP-N-acetyl-alpha-D-glucosamine</name>
            <dbReference type="ChEBI" id="CHEBI:57705"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="125"/>
        </location>
        <ligand>
            <name>UDP-N-acetyl-alpha-D-glucosamine</name>
            <dbReference type="ChEBI" id="CHEBI:57705"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="161"/>
        </location>
        <ligand>
            <name>UDP-N-acetyl-alpha-D-glucosamine</name>
            <dbReference type="ChEBI" id="CHEBI:57705"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="189"/>
        </location>
        <ligand>
            <name>UDP-N-acetyl-alpha-D-glucosamine</name>
            <dbReference type="ChEBI" id="CHEBI:57705"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="243"/>
        </location>
        <ligand>
            <name>UDP-N-acetyl-alpha-D-glucosamine</name>
            <dbReference type="ChEBI" id="CHEBI:57705"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="288"/>
        </location>
        <ligand>
            <name>UDP-N-acetyl-alpha-D-glucosamine</name>
            <dbReference type="ChEBI" id="CHEBI:57705"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00033"/>
        </source>
    </evidence>
    <sequence length="356" mass="37429" checksum="28DA471627E479BF" modified="2006-05-30" version="1">MTARTLLVMAGGTGGHVFPGLAVARALRDEGWRVVWLGNRTGMEATLVPKHDIPMEYIQFGGLRGKGLLTKLLLPLNLLRAFWQSIGALRRVKPDVVLGMGGYITFPAGMMASLLGRPLVLHEQNSIAGLANKVLAKVADRVLCAFPDALPNSEWTGNPVRAELAQIPAPESRYDHRAGPLHVLVVGGSLGAAALNDVVPKAIALLPEGQRPVVKHQAGAKQIDTLRANYAAAGVAGDTVPFIDDMAAAYADADLVICRAGAMTVSEVAAAGVAALFVPFPHAVDDHQTTNATFLSKQGAALLVQQNELTAEGLAKTLAGLSRTQLKDMARAARGLAKPEATRRVAEICSQLAGKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="72" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LJ39</accession>
    <name>Q1LJ39_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Proton-translocating NAD(P)(+) transhydrogenase</fullName>
            <ecNumber evidence="3">7.1.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">pntAb</name>
        <name evidence="12" type="ordered locus">Rmet_2964</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">The transhydrogenation between NADH and NADP is coupled to respiration and ATP hydrolysis and functions as a proton pump across the membrane.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>H(+)(in) + NAD(+) + NADPH = H(+)(out) + NADH + NADP(+)</text>
            <dbReference type="Rhea" id="RHEA:47992"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="7.1.1.1"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="7.1.1.1" evidence="3"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09837.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008641884.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2964"/>
    <dbReference type="EnsemblBacteria" id="ABF09837">
        <property type="protein sequence ID" value="ABF09837"/>
        <property type="gene ID" value="Rmet_2964"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2964"/>
    <dbReference type="eggNOG" id="COG3288">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_137885_2_0_4"/>
    <dbReference type="OMA" id="IITRRML"/>
    <dbReference type="OrthoDB" id="1933548at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024605">
        <property type="entry name" value="NADP_transhyd_a_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12769">
        <property type="entry name" value="PNTB_4TM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0520">NAD</keyword>
    <keyword evidence="5" id="KW-0521">NADP</keyword>
    <keyword evidence="12" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-1278">Translocase</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="38"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="64"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="domain" description="PNTB_4TM" evidence="11">
        <location>
            <begin position="11"/>
            <end position="94"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00003943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF12769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ABF09837.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="107" mass="11252" checksum="869347DC278AAF6A" modified="2006-05-30" version="1">MEMVNHTVINLIIFVLAIYVGYHVVWTVTPALHTPLMAVTNAISAIIIVGAMLAAGLTEGGVGRVMGTVAVALAAVNVFGGFLVTQRMLEMFKKKEPKAKADNKGGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LJ79</accession>
    <name>Q1LJ79_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">D-Lactate dehydrogenase (Cytochrome)</fullName>
            <ecNumber evidence="2">1.1.2.4</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">dld</name>
        <name evidence="2" type="ordered locus">Rmet_2924</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EC" id="1.1.2.4" evidence="2"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09797.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2924"/>
    <dbReference type="EnsemblBacteria" id="ABF09797">
        <property type="protein sequence ID" value="ABF09797"/>
        <property type="gene ID" value="Rmet_2924"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2924"/>
    <dbReference type="eggNOG" id="COG0277">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017779_3_0_4"/>
    <dbReference type="OMA" id="MLLMEFH"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004458">
        <property type="term" value="F:D-lactate dehydrogenase (cytochrome) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.45.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.465.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016166">
        <property type="entry name" value="FAD-bd_PCMH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036318">
        <property type="entry name" value="FAD-bd_PCMH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016169">
        <property type="entry name" value="FAD-bd_PCMH_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016164">
        <property type="entry name" value="FAD-linked_Oxase-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004113">
        <property type="entry name" value="FAD-linked_oxidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006094">
        <property type="entry name" value="Oxid_FAD_bind_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016171">
        <property type="entry name" value="Vanillyl_alc_oxidase_C-sub2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02913">
        <property type="entry name" value="FAD-oxidase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01565">
        <property type="entry name" value="FAD_binding_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55103">
        <property type="entry name" value="SSF55103"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56176">
        <property type="entry name" value="SSF56176"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51387">
        <property type="entry name" value="FAD_PCMH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD-binding PCMH-type" evidence="1">
        <location>
            <begin position="72"/>
            <end position="249"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF09797.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="495" mass="53525" checksum="EF43A6E9683FDBB3" modified="2006-05-30" version="1">MEGDVEWRHSLAYRAIRPEPETRMNHPTPATALARRPFPSELADALAARFGDRFTTSVGVREHHGRDESPFPPELPDAVVFAHSTEEVAELAKLCNTHGVPLIPYGAGSSLEGHLLAVAGGISLDLSQMNRVLAVHPEDLTVTVQPGVTRKQLNQEIKDTGLFFPIDPGADASLGGMCATRASGTNAVRYGTMRENVLALTVVTADGRVIRTGTNARKSSAGYDLTRLFVGSEGTLGIITEVTVRLYPQPEAISAAVCAFPSMGDAVRATIQTIQLGVPVARVEFVDALAIRAINRHDNLTLPETPHLFFEFHGTEAGVREQAETVQDIAAEHGGQGFEWATRPEDRSRLWNARHTAYFAMLQLKPGCKSVTTDVCVPISRLADCVTETERDLNASSLPCPIVGHVGDGNFHVAILVDPDKPEEMAEAEDINRRIVQRALAMGGTCTGEHGVGLHKMGFLVQEHGEDALDLMRAIKDALDPNHILNPGKIFSATR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LJM0</accession>
    <name>Q1LJM0_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Alpha-ribazole phosphatase, Phosphoglycerate mutase family protein</fullName>
            <ecNumber evidence="2">3.1.3.73</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_2783</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EC" id="3.1.3.73" evidence="2"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09656.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517354.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2783"/>
    <dbReference type="EnsemblBacteria" id="ABF09656">
        <property type="protein sequence ID" value="ABF09656"/>
        <property type="gene ID" value="Rmet_2783"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2783"/>
    <dbReference type="eggNOG" id="COG0406">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033323_8_3_4"/>
    <dbReference type="OMA" id="IRHTSVD"/>
    <dbReference type="OrthoDB" id="1122642at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043755">
        <property type="term" value="F:alpha-ribazole phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006094">
        <property type="term" value="P:gluconeogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd07067">
        <property type="entry name" value="HP_PGM_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1240">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013078">
        <property type="entry name" value="His_Pase_superF_clade-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029033">
        <property type="entry name" value="His_PPase_superfam"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00300">
        <property type="entry name" value="His_Phos_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00855">
        <property type="entry name" value="PGAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53254">
        <property type="entry name" value="SSF53254"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0312">Gluconeogenesis</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF09656.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="195" mass="21385" checksum="9213FA7619F86BB8" modified="2006-05-30" version="1">MEVVLIRHVRPDVAPGVCYGALDLKLAQPVSPAPERIVRLLGDLPPSRLVTSPAVRATETAALLSAHVEMPVREIEPRLRELDFGAWEGKAWSEIPRDALDLWAADLMGACPHGGESAQQVMARVTAWADTLDASSGDCLWAVSHAGPMRMLAAHWLGLTLSQTLQWELEFGATCHFHLGKRQARLGWWNRSAKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LJN1</accession>
    <name>Q1LJN1_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Outer membrane protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Rmet_2772</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell outer membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09645.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2772"/>
    <dbReference type="EnsemblBacteria" id="ABF09645">
        <property type="protein sequence ID" value="ABF09645"/>
        <property type="gene ID" value="Rmet_2772"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2772"/>
    <dbReference type="eggNOG" id="COG0729">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018618_2_0_4"/>
    <dbReference type="OMA" id="NTTMLLY"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009279">
        <property type="term" value="C:cell outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000184">
        <property type="entry name" value="Bac_surfAg_D15"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010827">
        <property type="entry name" value="BamA/TamA_POTRA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039910">
        <property type="entry name" value="D15-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12815">
        <property type="entry name" value="PTHR12815"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01103">
        <property type="entry name" value="Omp85"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07244">
        <property type="entry name" value="POTRA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1134">Transmembrane beta strand</keyword>
    <feature type="domain" description="POTRA" evidence="6">
        <location>
            <begin position="280"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="domain" description="Omp85" evidence="5">
        <location>
            <begin position="498"/>
            <end position="684"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="344"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="355"/>
            <end position="371"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004442"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF07244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABF09645.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="696" mass="76808" checksum="E655E68855EB369F" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="99" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LJR4</accession>
    <name>Q1LJR4_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Dihydroorotase (DHOase)</fullName>
            <ecNumber evidence="3">3.5.2.3</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">pyrX</name>
        <name evidence="3" type="ordered locus">Rmet_2739</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EC" id="3.5.2.3" evidence="3"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09612.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2739"/>
    <dbReference type="EnsemblBacteria" id="ABF09612">
        <property type="protein sequence ID" value="ABF09612"/>
        <property type="gene ID" value="Rmet_2739"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2739"/>
    <dbReference type="eggNOG" id="COG0044">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015572_1_0_4"/>
    <dbReference type="OMA" id="QHAQEPR"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004151">
        <property type="term" value="F:dihydroorotase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006221">
        <property type="term" value="P:pyrimidine nucleotide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01317">
        <property type="entry name" value="DHOase_IIa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006680">
        <property type="entry name" value="Amidohydro-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004722">
        <property type="entry name" value="DHOase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011059">
        <property type="entry name" value="Metal-dep_hydrolase_composite"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01979">
        <property type="entry name" value="Amidohydro_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51338">
        <property type="entry name" value="SSF51338"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="SSF51556"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Amidohydro-rel" evidence="2">
        <location>
            <begin position="239"/>
            <end position="435"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF09612.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="439" mass="47259" checksum="9C92F8D22360F8E2" modified="2006-05-30" version="1">MSRTPDTTNSTHRQNMKIHIQGGRLIDPAANVDAEQDLYIAAGKIVGVGRAPTDFHANKTIDARGMIVCPGLVDLSARLREPGYEYKATLESELAAATAGGVTSLVCPPDTDPVLDEPGLVEMLKFRAHKLNQTHVYPLGALTVGLKGDALTEMGQLTEAGCVGFSQAEAPIHDTKVLQRAMQYAQTFGFTLWLRPEDPFLGGGVAASGAVASRLGLSGVSVIAETVRLHTIFELIRATGARVHLCRLSSAAGLELVRQARKEGLPVTCDVNIHHVSLTDMDIGFFNSQMRFTPPLRGARDRDAIVAALADGTIDALCSDHTPVDDDEKLLPFAEASPGATGLELLLPLTLRWASEHKVPLVRALARITSEPARVIGLNVGSLTTGSMADVCVFDPKQLWKVERSVLKSQGKNSPWLGYEMEGRVRTTLVCGQVVYEQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="104" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LKG6</accession>
    <name>Q1LKG6_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Succinate dehydrogenase iron-sulfur subunit</fullName>
            <ecNumber evidence="7">1.3.5.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">sdhB</name>
        <name evidence="10" type="ordered locus">Rmet_2483</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>a quinone + succinate = a quinol + fumarate</text>
            <dbReference type="Rhea" id="RHEA:40523"/>
            <dbReference type="ChEBI" id="CHEBI:24646"/>
            <dbReference type="ChEBI" id="CHEBI:29806"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:132124"/>
            <dbReference type="EC" id="1.3.5.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="7">
            <name>2Fe-2S cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49601"/>
        </cofactor>
        <text evidence="7">Binds 1 [2Fe-2S] cluster.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="7">
            <name>[3Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:21137"/>
        </cofactor>
        <text evidence="7">Binds 1 [3Fe-4S] cluster.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="7">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="7">Binds 1 [4Fe-4S] cluster.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the succinate dehydrogenase/fumarate reductase iron-sulfur protein family.</text>
    </comment>
    <dbReference type="EC" id="1.3.5.1" evidence="7"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09360.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008649360.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2483"/>
    <dbReference type="EnsemblBacteria" id="ABF09360">
        <property type="protein sequence ID" value="ABF09360"/>
        <property type="gene ID" value="Rmet_2483"/>
    </dbReference>
    <dbReference type="GeneID" id="60821042"/>
    <dbReference type="KEGG" id="rme:Rmet_2483"/>
    <dbReference type="eggNOG" id="COG0479">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_044838_0_2_4"/>
    <dbReference type="OMA" id="DGQYFGP"/>
    <dbReference type="OrthoDB" id="1605514at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051538">
        <property type="term" value="F:3 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008177">
        <property type="term" value="F:succinate dehydrogenase (ubiquinone) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1060.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036010">
        <property type="entry name" value="2Fe-2S_ferredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001041">
        <property type="entry name" value="2Fe-2S_ferredoxin-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012675">
        <property type="entry name" value="Beta-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009051">
        <property type="entry name" value="Helical_ferredxn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004489">
        <property type="entry name" value="Succ_DH/fum_Rdtase_Fe-S"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025192">
        <property type="entry name" value="Succ_DH/fum_Rdtase_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13085">
        <property type="entry name" value="Fer2_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54292">
        <property type="entry name" value="SSF54292"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00384">
        <property type="entry name" value="dhsB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51085">
        <property type="entry name" value="2FE2S_FER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0001">2Fe-2S</keyword>
    <keyword evidence="6 7" id="KW-0003">3Fe-4S</keyword>
    <keyword evidence="7" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="4 7" id="KW-0408">Iron</keyword>
    <keyword evidence="5 7" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="3 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0816">Tricarboxylic acid cycle</keyword>
    <feature type="domain" description="2Fe-2S ferredoxin-type" evidence="8">
        <location>
            <begin position="5"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="9">
        <location>
            <begin position="134"/>
            <end position="164"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU361237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ABF09360.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="233" mass="26752" checksum="EE144349C6DE601E" modified="2006-05-30" version="1">MKRIFEVYRYDPDKDAAPRMQTYEVELDGHERMLLDALVKLKKLDETISFRRSCREGVCGSDAMNINGKNGLACLTNMRELPDRIVLRPLPGLPVVRDLIVDMTNFFKQYNSIKPFLINDEPPPEKERLQSPQERDELDGLYECILCASCSTSCPSFWWNPDKFVGPAGLLQAYRFIADSRDQATSERLDNLNDPYRLFRCHSIMNCVDVCPKGLNPTKAIGKIKELMVRRAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-12-02" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LKS3</accession>
    <name>Q1LKS3_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Cytochrome C oxidase, mono-heme subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">ccoO</name>
        <name evidence="6" type="ordered locus">Rmet_2376</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09253.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2376"/>
    <dbReference type="EnsemblBacteria" id="ABF09253">
        <property type="protein sequence ID" value="ABF09253"/>
        <property type="gene ID" value="Rmet_2376"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2376"/>
    <dbReference type="eggNOG" id="COG2993">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_050647_2_0_4"/>
    <dbReference type="OMA" id="PRAIFDW"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.760.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009056">
        <property type="entry name" value="Cyt_c-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036909">
        <property type="entry name" value="Cyt_c-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003468">
        <property type="entry name" value="Cyt_c_oxidase_monohaem-su/FixO"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02433">
        <property type="entry name" value="FixO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46626">
        <property type="entry name" value="SSF46626"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51007">
        <property type="entry name" value="CYTC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 4" id="KW-0349">Heme</keyword>
    <keyword evidence="3 4" id="KW-0408">Iron</keyword>
    <keyword evidence="2 4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cytochrome c" evidence="5">
        <location>
            <begin position="24"/>
            <end position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABF09253.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="193" mass="21614" checksum="FC17A9B3358B257C" modified="2006-05-30" version="1">MLVVLPGLQVRTVQAPAQLQNYTIEQLRGRAQYVANGCVYCHSQQPRSSGQTLADQARGWGRPSTPGDYAYDAPQLLGTMRTGPDLLNVGVRLPSRDWQLTHLYQPRAIFDWSIMPSYPFLFEVKEKAAPGEVVVKLPQEYRPADGKVVVARQEALDLVAYLLSLDRSYPVSAKEATLRDRGYDPKTQKGPRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="70" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LKW5</accession>
    <name>Q1LKW5_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative lytic transglycosylase, catalytic</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_2334</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09211.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_003109780.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2334"/>
    <dbReference type="CAZy" id="GH23">
        <property type="family name" value="Glycoside Hydrolase Family 23"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF09211">
        <property type="protein sequence ID" value="ABF09211"/>
        <property type="gene ID" value="Rmet_2334"/>
    </dbReference>
    <dbReference type="GeneID" id="56409841"/>
    <dbReference type="KEGG" id="rme:Rmet_2334"/>
    <dbReference type="eggNOG" id="COG0741">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_063182_2_2_4"/>
    <dbReference type="OMA" id="HAGPNNN"/>
    <dbReference type="OrthoDB" id="1444566at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023346">
        <property type="entry name" value="Lysozyme-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008258">
        <property type="entry name" value="Transglycosylase_SLT_dom_1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01464">
        <property type="entry name" value="SLT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53955">
        <property type="entry name" value="SSF53955"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193640" evidence="1">
        <location>
            <begin position="27"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="domain" description="SLT" evidence="2">
        <location>
            <begin position="36"/>
            <end position="157"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF09211.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="196" mass="21095" checksum="FDC5F14110ACDED6" modified="2006-05-30" version="1">MAAPAVAMPLRALVLTAGLYAVAALAQEVPPPAYQLAAQRAGIPSTVLYAVALQESGIRRNGRLVPWPWSLNVAGQSRRFATRADACAGLQQAMRATPHTRIDAGLGQINLGYHKHRFTGACDLLDPYRNLAVAAEILKEQHTPGEDWLLAIGRYHRPAGGEPAARYRRSVSRHLARVQGTRPAAAVLAARQETSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="108" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LL23</accession>
    <name>Q1LL23_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 7">Glutamate racemase</fullName>
            <ecNumber evidence="2 7">5.1.1.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7 8" type="primary">murI</name>
        <name evidence="8" type="ordered locus">Rmet_2274</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Provides the (R)-glutamate required for cell wall biosynthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 7">
            <text>L-glutamate = D-glutamate</text>
            <dbReference type="Rhea" id="RHEA:12813"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:29986"/>
            <dbReference type="EC" id="5.1.1.3"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="7">Cell wall biogenesis; peptidoglycan biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the aspartate/glutamate racemases family.</text>
    </comment>
    <dbReference type="EC" id="5.1.1.3" evidence="2 7"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09153.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516979.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2274"/>
    <dbReference type="EnsemblBacteria" id="ABF09153">
        <property type="protein sequence ID" value="ABF09153"/>
        <property type="gene ID" value="Rmet_2274"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2274"/>
    <dbReference type="eggNOG" id="COG0796">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_052344_2_1_4"/>
    <dbReference type="OMA" id="MPWGPRT"/>
    <dbReference type="OrthoDB" id="1718671at2"/>
    <dbReference type="UniPathway" id="UPA00219"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008881">
        <property type="term" value="F:glutamate racemase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009252">
        <property type="term" value="P:peptidoglycan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008360">
        <property type="term" value="P:regulation of cell shape"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00258">
        <property type="entry name" value="Glu_racemase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015942">
        <property type="entry name" value="Asp/Glu/hydantoin_racemase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001920">
        <property type="entry name" value="Asp/Glu_race"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033134">
        <property type="entry name" value="Asp/Glu_racemase_AS_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004391">
        <property type="entry name" value="Glu_race"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21198:SF2">
        <property type="entry name" value="PTHR21198:SF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01177">
        <property type="entry name" value="Asp_Glu_race"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53681">
        <property type="entry name" value="SSF53681"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00067">
        <property type="entry name" value="glut_race"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00924">
        <property type="entry name" value="ASP_GLU_RACEMASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7" id="KW-0133">Cell shape</keyword>
    <keyword evidence="6 7" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="5 7 8" id="KW-0413">Isomerase</keyword>
    <keyword evidence="4 7" id="KW-0573">Peptidoglycan synthesis</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Substrate binding" evidence="7">
        <location>
            <begin position="10"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="7">
        <location>
            <begin position="42"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="7">
        <location>
            <begin position="74"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="7">
        <location>
            <begin position="184"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="7">
        <location>
            <position position="73"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="7">
        <location>
            <position position="183"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001602"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00258"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ABF09153.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="270" mass="28700" checksum="27D9FAE8FF69ABD1" modified="2006-05-30" version="1">MNPSPVGIFDSGLGGLSVLREVRALLPHESLLYFADSRYAPYGEKPERFVEARTLQVCEWLVAQGCKALVIACNTATMHAVQTLRERLAVPIIGVEPGLKPAAAASRSKVVGVLATANTLKSAKFNRLLTSLGNESRFLCEAGVGLVPLIEQGDVSSDTVRQTLGTLLSPMLEAGADTLVLGCTHYPFLSETIDDLVDGRLALIDTGVAIARQLARKLAEFDLAASPDTAPSVRYLSTKDAAHLREMAAALLHVEANAETVVIEPAPATE</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="100" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LLE5</accession>
    <name>Q1LLE5_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Peptidoglycan glycosyltransferase</fullName>
            <ecNumber evidence="4">2.4.1.129</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="primary">mrcA1</name>
        <name evidence="19" type="ordered locus">Rmet_2152</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>[GlcNAc-(1-&gt;4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)](n)-di-trans,octa-cis-undecaprenyl diphosphate + beta-D-GlcNAc-(1-&gt;4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-di-trans,octa-cis-undecaprenyl diphosphate = [GlcNAc-(1-&gt;4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)](n+1)-di-trans-octa-cis-undecaprenyl diphosphate + di-trans,octa-cis-undecaprenyl diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:23708"/>
            <dbReference type="Rhea" id="RHEA-COMP:9602"/>
            <dbReference type="Rhea" id="RHEA-COMP:9603"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:58405"/>
            <dbReference type="ChEBI" id="CHEBI:60033"/>
            <dbReference type="ChEBI" id="CHEBI:78435"/>
            <dbReference type="EC" id="2.4.1.129"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cell wall biogenesis; peptidoglycan biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">In the C-terminal section; belongs to the transpeptidase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">In the N-terminal section; belongs to the glycosyltransferase 51 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.129" evidence="4"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09031.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2152"/>
    <dbReference type="CAZy" id="GT51">
        <property type="family name" value="Glycosyltransferase Family 51"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF09031">
        <property type="protein sequence ID" value="ABF09031"/>
        <property type="gene ID" value="Rmet_2152"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2152"/>
    <dbReference type="eggNOG" id="COG5009">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006354_2_4_4"/>
    <dbReference type="OMA" id="LAQMAMI"/>
    <dbReference type="OrthoDB" id="652304at2"/>
    <dbReference type="UniPathway" id="UPA00219"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004180">
        <property type="term" value="F:carboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008658">
        <property type="term" value="F:penicillin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008955">
        <property type="term" value="F:peptidoglycan glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009252">
        <property type="term" value="P:peptidoglycan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008360">
        <property type="term" value="P:regulation of cell shape"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3810.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.710.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012338">
        <property type="entry name" value="Beta-lactam/transpept-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001264">
        <property type="entry name" value="Glyco_trans_51"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023346">
        <property type="entry name" value="Lysozyme-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036950">
        <property type="entry name" value="PBP_transglycosylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001460">
        <property type="entry name" value="PCN-bd_Tpept"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00912">
        <property type="entry name" value="Transgly"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00905">
        <property type="entry name" value="Transpeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53955">
        <property type="entry name" value="SSF53955"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56601">
        <property type="entry name" value="SSF56601"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0121">Carboxypeptidase</keyword>
    <keyword evidence="10" id="KW-0133">Cell shape</keyword>
    <keyword evidence="14" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="7" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="16" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="11" id="KW-0573">Peptidoglycan synthesis</keyword>
    <keyword evidence="6" id="KW-0645">Protease</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <keyword evidence="8 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12 16" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="21"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="domain" description="Transgly" evidence="18">
        <location>
            <begin position="72"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="domain" description="Transpeptidase" evidence="17">
        <location>
            <begin position="419"/>
            <end position="662"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004752"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007739"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023988"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF00905"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF00912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="ABF09031.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="773" mass="84940" checksum="0987224FC41CB723" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="62" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LLQ2</accession>
    <name>Q1LLQ2_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Cbb3-type cytochrome oxidase, maturation protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">rdxS</name>
        <name evidence="2" type="ordered locus">Rmet_2045</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08924.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008647465.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2045"/>
    <dbReference type="EnsemblBacteria" id="ABF08924">
        <property type="protein sequence ID" value="ABF08924"/>
        <property type="gene ID" value="Rmet_2045"/>
    </dbReference>
    <dbReference type="GeneID" id="60821376"/>
    <dbReference type="KEGG" id="rme:Rmet_2045"/>
    <dbReference type="eggNOG" id="COG3197">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
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    <dbReference type="OrthoDB" id="2095758at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004714">
        <property type="entry name" value="Cyt_oxidase_maturation_cbb3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR41532">
        <property type="entry name" value="PTHR41532"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03597">
        <property type="entry name" value="FixS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00847">
        <property type="entry name" value="ccoS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF08924.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="48" mass="5322" checksum="CFD3172D42619621" modified="2006-05-30" version="1">METLYLLVPMSLVLVAVIAGALWWALHAGQYDDLDRPAEAILLDNDKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LLU2</accession>
    <name>Q1LLU2_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_2005</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08884.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516723.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2005"/>
    <dbReference type="EnsemblBacteria" id="ABF08884">
        <property type="protein sequence ID" value="ABF08884"/>
        <property type="gene ID" value="Rmet_2005"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2005"/>
    <dbReference type="HOGENOM" id="CLU_2303681_0_0_4"/>
    <dbReference type="OrthoDB" id="1768791at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF08884.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="100" mass="11562" checksum="248A6D788B4D73DD" modified="2006-05-30" version="1">MVDDLLARLYRVREAGYSQWLACCPACQEPGRTLLIRSNSDGFTLIHCRNGCPPGFVLHAAGVPWSVLFSDGAQRRHAWPPEWWREPPRYEREPRPMVEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="112" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LM92</accession>
    <name>Q1LM92_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">3-hydroxyacyl-CoA dehydrogenase</fullName>
            <ecNumber evidence="2">1.1.1.35</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">fadB</name>
        <name evidence="11" type="ordered locus">Rmet_1855</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>a (3S)-3-hydroxyacyl-CoA + NAD(+) = a 3-oxoacyl-CoA + H(+) + NADH</text>
            <dbReference type="Rhea" id="RHEA:22432"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57318"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:90726"/>
            <dbReference type="EC" id="1.1.1.35"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Lipid metabolism; fatty acid beta-oxidation.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.35" evidence="2"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08734.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008650235.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1855"/>
    <dbReference type="EnsemblBacteria" id="ABF08734">
        <property type="protein sequence ID" value="ABF08734"/>
        <property type="gene ID" value="Rmet_1855"/>
    </dbReference>
    <dbReference type="GeneID" id="60825652"/>
    <dbReference type="KEGG" id="rme:Rmet_1855"/>
    <dbReference type="eggNOG" id="COG1024">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG1250">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009834_16_3_4"/>
    <dbReference type="OMA" id="TGAGWPF"/>
    <dbReference type="OrthoDB" id="977512at2"/>
    <dbReference type="UniPathway" id="UPA00659"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003857">
        <property type="term" value="F:3-hydroxyacyl-CoA dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016853">
        <property type="term" value="F:isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070403">
        <property type="term" value="F:NAD+ binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006635">
        <property type="term" value="P:fatty acid beta-oxidation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006176">
        <property type="entry name" value="3-OHacyl-CoA_DH_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006108">
        <property type="entry name" value="3HC_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008927">
        <property type="entry name" value="6-PGluconate_DH-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029045">
        <property type="entry name" value="ClpP/crotonase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001753">
        <property type="entry name" value="Enoyl-CoA_hydra/iso"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00725">
        <property type="entry name" value="3HCDH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02737">
        <property type="entry name" value="3HCDH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00378">
        <property type="entry name" value="ECH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48179">
        <property type="entry name" value="SSF48179"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52096">
        <property type="entry name" value="SSF52096"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="11" id="KW-0413">Isomerase</keyword>
    <keyword evidence="5" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="7" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="11" id="KW-0456">Lyase</keyword>
    <keyword evidence="6" id="KW-0520">NAD</keyword>
    <keyword evidence="11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="3HCDH_N" evidence="10">
        <location>
            <begin position="295"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="domain" description="3HCDH" evidence="9">
        <location>
            <begin position="475"/>
            <end position="567"/>
        </location>
    </feature>
    <feature type="domain" description="3HCDH" evidence="9">
        <location>
            <begin position="603"/>
            <end position="689"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013000"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023693"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF02737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ABF08734.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="693" mass="74757" checksum="8952F33738F052CE" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LMB7</accession>
    <name>Q1LMB7_CUPMC</name>
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        <submittedName>
            <fullName evidence="2">Putative glycine cleavage T protein (Aminomethyl transferase)</fullName>
            <ecNumber evidence="2">2.1.2.10</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">gcvT</name>
        <name evidence="2" type="ordered locus">Rmet_1830</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.1.2.10" evidence="2"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08709.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516559.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1830"/>
    <dbReference type="EnsemblBacteria" id="ABF08709">
        <property type="protein sequence ID" value="ABF08709"/>
        <property type="gene ID" value="Rmet_1830"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1830"/>
    <dbReference type="eggNOG" id="COG0354">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007884_6_2_4"/>
    <dbReference type="OMA" id="VNFKKGC"/>
    <dbReference type="OrthoDB" id="1984573at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004047">
        <property type="term" value="F:aminomethyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006222">
        <property type="entry name" value="GCV_T_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045179">
        <property type="entry name" value="YgfZ/GcvT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017703">
        <property type="entry name" value="YgfZ/GcvT_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22602">
        <property type="entry name" value="PTHR22602"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01571">
        <property type="entry name" value="GCV_T"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03317">
        <property type="entry name" value="ygfZ_signature"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="GCV_T" evidence="1">
        <location>
            <begin position="28"/>
            <end position="183"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01571"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF08709.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="336" mass="35177" checksum="420F63736ED1CBDF" modified="2006-05-30" version="1">MSSTEFPSLDQSLKSLRAAGIVTAPGGLGLIRVAGDDAATFLHSQLTNAVEDLTASTARLAGYCSPKGRLMASFLMWRDADGIVLQLSADIQPPIQKRLTMFVLRAKAKLSDLSATHRILGIAGAGAEAALQQAGLPTPQAPLATASDDNVTVIRLADADGEPRWQIVAPAARIEALQQSLGATLAVASPAFWDWLDVASGIPRIAAATQEQFVPQMINFELIGGVNFRKGCYPGQEVVARSQYRGTLKRRMWRVRGTGDVPAAAAEIFRPEDPEQPCGMLVNAAPAPQGGWEGLAELKIDAANGALHLGAASGPAVSTGALPYEVPLPNEAEAAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LMI5</accession>
    <name>Q1LMI5_CUPMC</name>
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        <submittedName>
            <fullName evidence="7">Putative membrane protein fused with conserved domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">yoaE</name>
        <name evidence="7" type="ordered locus">Rmet_1762</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
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        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0053 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08641.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516494.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1762"/>
    <dbReference type="EnsemblBacteria" id="ABF08641">
        <property type="protein sequence ID" value="ABF08641"/>
        <property type="gene ID" value="Rmet_1762"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1762"/>
    <dbReference type="eggNOG" id="COG1253">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015237_0_0_4"/>
    <dbReference type="OMA" id="MRFINRH"/>
    <dbReference type="OrthoDB" id="1917125at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04590">
        <property type="entry name" value="CBS_pair_CorC_HlyC_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.580.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.465.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036318">
        <property type="entry name" value="FAD-bd_PCMH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016169">
        <property type="entry name" value="FAD-bd_PCMH_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005496">
        <property type="entry name" value="Integral_membrane_TerC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044751">
        <property type="entry name" value="Ion_transp-like_CBS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005170">
        <property type="entry name" value="Transptr-assoc_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00571">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03471">
        <property type="entry name" value="CorC_HlyC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03741">
        <property type="entry name" value="TerC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01091">
        <property type="entry name" value="CorC_HlyC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="SSF54631"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56176">
        <property type="entry name" value="SSF56176"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51371">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0129">CBS domain</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="12"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="48"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="81"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="124"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="154"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="184"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="6">
        <location>
            <begin position="367"/>
            <end position="426"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006337"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABF08641.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="530" mass="57346" checksum="F401EC4C1303FA96" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="60" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LMQ0</accession>
    <name>Q1LMQ0_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_1695</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08576.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1695"/>
    <dbReference type="EnsemblBacteria" id="ABF08576">
        <property type="protein sequence ID" value="ABF08576"/>
        <property type="gene ID" value="Rmet_1695"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1695"/>
    <dbReference type="eggNOG" id="COG3064">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024625_0_1_4"/>
    <dbReference type="OMA" id="AIVDWDD"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021728">
        <property type="entry name" value="DUF3300"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40269">
        <property type="entry name" value="PTHR40269"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11737">
        <property type="entry name" value="DUF3300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193624" evidence="2">
        <location>
            <begin position="25"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="271"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="271"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="302"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="433"/>
            <end position="447"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF08576.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="489" mass="50182" checksum="A6E354462D7B2973" modified="2006-05-30" version="1">MRRLSWIGAWIIACFLASIGSAWAQENSAGPASFKPEELEALVAPIALYPDSVLAQALMASTYPLEVVQAARWVKANPNVKGDAAIKAVESQPWDVSVKSLVAFPQVLEPMSDKLDWTQKLGDAFLAQEKDVMAAVQRLRAKAQQSGNLKSNEQQKVIVEPPAQASGGTTGGGGTTQTIVRIEPANPEVIYVPSYNPTVVYGGWSYPSYPPYYWPPSPYYYPGGALMTGFAWGVGLAAAGAIFSNCNWGGGDVNINVNKAANIDRNFDRTKVQNGNRWQHDSSHRKGVAYRDNATREKFGNNVGGADRRNDFRGREGGRDGAGDRAGDRAGNRQGDRAGDRAGDRGGDRGGDRGGDRGGNRASTGDRMGGSDRAGVADRSAGGGDRGGAGVGDRSAGVADRSAGGGNRNSYGGGGRDNAFQGVGGGSTAQRDLDRGRASSSQAGFSGGQRGGGGGGGGFSGGGGRGGGGFSGGGGRGGGGGGRGGGGRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LMZ4</accession>
    <name>Q1LMZ4_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Cytochrome c, class I</fullName>
            <ecNumber evidence="13">1.1.99.3</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ordered locus">Rmet_1599</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>heme c</name>
            <dbReference type="ChEBI" id="CHEBI:61717"/>
        </cofactor>
        <text evidence="8">Binds 3 heme c groups covalently per subunit.</text>
    </comment>
    <comment type="PTM">
        <text evidence="9">Binds 3 heme groups per subunit.</text>
    </comment>
    <dbReference type="EC" id="1.1.99.3" evidence="13"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08482.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516329.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1599"/>
    <dbReference type="EnsemblBacteria" id="ABF08482">
        <property type="protein sequence ID" value="ABF08482"/>
        <property type="gene ID" value="Rmet_1599"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1599"/>
    <dbReference type="eggNOG" id="COG2010">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028594_0_0_4"/>
    <dbReference type="OMA" id="DGWYAPS"/>
    <dbReference type="OrthoDB" id="1297285at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033717">
        <property type="term" value="F:gluconate 2-dehydrogenase (acceptor) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.760.10">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009056">
        <property type="entry name" value="Cyt_c-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036909">
        <property type="entry name" value="Cyt_c-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014353">
        <property type="entry name" value="Membr-bd_ADH_cyt_c"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00034">
        <property type="entry name" value="Cytochrom_C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13442">
        <property type="entry name" value="Cytochrome_CBB3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000018">
        <property type="entry name" value="Mb_ADH_cyt_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46626">
        <property type="entry name" value="SSF46626"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51007">
        <property type="entry name" value="CYTC"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="2 8 10" id="KW-0349">Heme</keyword>
    <keyword evidence="6 9 10" id="KW-0408">Iron</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 9 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 11" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193231" evidence="11">
        <location>
            <begin position="32"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="domain" description="Cytochrome c" evidence="12">
        <location>
            <begin position="39"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="domain" description="Cytochrome c" evidence="12">
        <location>
            <begin position="185"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="domain" description="Cytochrome c" evidence="12">
        <location>
            <begin position="321"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="binding site" description="covalent" evidence="8">
        <location>
            <position position="53"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" description="covalent" evidence="8">
        <location>
            <position position="56"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="9">
        <location>
            <position position="57"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
            <label>1</label>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <feature type="binding site" description="covalent" evidence="8">
        <location>
            <position position="200"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" description="covalent" evidence="8">
        <location>
            <position position="203"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="9">
        <location>
            <position position="204"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
            <label>2</label>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <feature type="binding site" description="covalent" evidence="8">
        <location>
            <position position="334"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
            <label>3</label>
        </ligand>
    </feature>
    <feature type="binding site" description="covalent" evidence="8">
        <location>
            <position position="337"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
            <label>3</label>
        </ligand>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="9">
        <location>
            <position position="338"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
            <label>3</label>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR000018-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR000018-51"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ABF08482.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="438" mass="47549" checksum="AF3E461E2354FD78" modified="2006-05-30" version="1">MHHNKNGTAMRGFAIGWLAWMTWLSVSTAWAAPPTASQAQVERGRYLARVANCVACHTAEGGAPMAGGLPLQTGAGTVYSTNITPEASTGLGRYSLDDFDRAVRRGVARDGRRLYPAMPYPSYARMTRDDVAAVYAWLRAEVKPVAQTNRDAQMRWPYSMRWLMVPWNLLHLEEGPIPASADRGAAWNRGAYLVQAVAHCGACHTPRGTLFGEKGTDTRSREFLSGATVEGWSATNLTGDMLTGLGAWSLAEIVEYLHTGRNAHATSFGPMSTVIASSTQYMTPGDLDAVATYLKSIPGARREAEPFRYDATVATQLEQGRFDTAGARQYATYCMPCHNASGKGYERVFPPLAGNPTVVDPNPASLVNLLLNGAVTARVNTAPTDYHMPGYGWTMEDQELAHLLTFIRSSWGNRAAPVTAQTVAERRKATGKQATNAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="107" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LMZ7</accession>
    <name>Q1LMZ7_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">DNA translocase ftsK, DNA segregation ATPase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">ftsK</name>
        <name evidence="8" type="ordered locus">Rmet_1596</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Essential cell division protein that coordinates cell division and chromosome segregation. The N-terminus is involved in assembly of the cell-division machinery. The C-terminus functions as a DNA motor that moves dsDNA in an ATP-dependent manner towards the dif recombination site, which is located within the replication terminus region. Translocation stops specifically at Xer-dif sites, where FtsK interacts with the Xer recombinase, allowing activation of chromosome unlinking by recombination. FtsK orienting polar sequences (KOPS) guide the direction of DNA translocation. FtsK can remove proteins from DNA as it translocates, but translocation stops specifically at XerCD-dif site, thereby preventing removal of XerC and XerD from dif.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the FtsK/SpoIIIE/SftA family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08479.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516326.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1596"/>
    <dbReference type="EnsemblBacteria" id="ABF08479">
        <property type="protein sequence ID" value="ABF08479"/>
        <property type="gene ID" value="Rmet_1596"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1596"/>
    <dbReference type="eggNOG" id="COG1674">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001981_14_1_4"/>
    <dbReference type="OMA" id="GETWRSL"/>
    <dbReference type="OrthoDB" id="349533at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041027">
        <property type="entry name" value="FtsK_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002543">
        <property type="entry name" value="FtsK_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018541">
        <property type="entry name" value="Ftsk_gamma"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17854">
        <property type="entry name" value="FtsK_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09397">
        <property type="entry name" value="FtsK_gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01580">
        <property type="entry name" value="FtsK_SpoIIIE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00843">
        <property type="entry name" value="Ftsk_gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50901">
        <property type="entry name" value="FTSK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="68"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="domain" description="FtsK" evidence="7">
        <location>
            <begin position="769"/>
            <end position="978"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="116"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="519"/>
            <end position="569"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="116"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="172"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="239"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="531"/>
            <end position="550"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="786"/>
            <end position="793"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006474"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00024784"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50901"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ABF08479.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="1123" mass="117232" checksum="062C44556248D84D" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LNG3</accession>
    <name>Q1LNG3_CUPMC</name>
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        <submittedName>
            <fullName evidence="7">Putative MFS transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Rmet_1430</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08313.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516181.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1430"/>
    <dbReference type="EnsemblBacteria" id="ABF08313">
        <property type="protein sequence ID" value="ABF08313"/>
        <property type="gene ID" value="Rmet_1430"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1430"/>
    <dbReference type="eggNOG" id="COG0477">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000960_28_2_4"/>
    <dbReference type="OMA" id="CAGQFLV"/>
    <dbReference type="OrthoDB" id="1108236at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="SSF103473"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="51"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="82"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="111"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="138"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="172"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="204"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="229"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="273"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="308"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="339"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="370"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="403"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="440"/>
            <end position="462"/>
        </location>
    </feature>
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        <location>
            <begin position="13"/>
            <end position="465"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABF08313.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="480" mass="50206" checksum="C200FFFED62F76FB" modified="2006-05-30" version="1">MQTSPAPTLGRAGLYVLLAGQLLPQIDFSIVNVALHPIEHSLGATPSELELMVAVYGVAFAVFLAMGGRLGDNYGRRRIFNIGVLLFGVASLLCGVAGSMLSLLIARTLQGAAAALLVPQILATIHVGLRGHAHSRALALFASIGGIAFIIGQVLGGLLVDADIDGLGWRNAFLINLPICLAILVLSPRVIPETRRENATGIDAPGTVLLAAVILAILLPIALGPMFRWSWPVLAVLAATAPLMLLLWYIELRQERRGVHPLLPPSLLRLPSVRFGFIVAVLFYACWSGLMFVLALTLQSGAGMTPTMAGNAFIPLGLAFIAGSLTSPRVVNGWGRTRVLIVGCVTQMAGVVVLAYVLRQAWPHPDILTLAPPSLLIGFGNALVVGCFYRIGLADIPTDQAGAGSAALATVQQASLGLGSALLGTVFAQRLHHAATYLDAALASLAAELCLMTILVAAALVYHNRHGQPAITACASPQSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LNS9</accession>
    <name>Q1LNS9_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Toluene-4-monooxygenase system protein C (Belongs to CMGI-2)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">tmoC</name>
        <name evidence="6" type="ordered locus">Rmet_1314</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08197.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516080.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1314"/>
    <dbReference type="EnsemblBacteria" id="ABF08197">
        <property type="protein sequence ID" value="ABF08197"/>
        <property type="gene ID" value="Rmet_1314"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1314"/>
    <dbReference type="eggNOG" id="COG2146">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_055690_5_0_4"/>
    <dbReference type="OMA" id="INPGDCR"/>
    <dbReference type="OrthoDB" id="1681886at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019439">
        <property type="term" value="P:aromatic compound catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.102.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017941">
        <property type="entry name" value="Rieske_2Fe-2S"/>
    </dbReference>
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        <property type="entry name" value="Rieske_2Fe-2S_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00355">
        <property type="entry name" value="Rieske"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="RIESKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0058">Aromatic hydrocarbons catabolism</keyword>
    <keyword evidence="3" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rieske" evidence="5">
        <location>
            <begin position="4"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022797"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABF08197.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="111" mass="12319" checksum="ACB334A021CE7D13" modified="2006-05-30" version="1">MTFKKVCSLDDLWEGEMESFEVDGQEILMVWPQGGDLKAFQGICPHQDIPLIEGKFDGKTVICRAHLWQFDACSGKGINPSDCALAEYPIKIEGDAVFVETEGVKPLFAHT</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="100" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LNT6</accession>
    <name>Q1LNT6_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Transcriptional regulatory protein with helix-turn-helix motif, Fis-type (Belongs to CMGI-2)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">patR</name>
        <name evidence="6" type="ordered locus">Rmet_1305</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08190.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1305"/>
    <dbReference type="EnsemblBacteria" id="ABF08190">
        <property type="protein sequence ID" value="ABF08190"/>
        <property type="gene ID" value="Rmet_1305"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1305"/>
    <dbReference type="eggNOG" id="COG1719">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG3829">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_119_2_4"/>
    <dbReference type="OMA" id="NVYPIRI"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002197">
        <property type="entry name" value="HTH_Fis"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024096">
        <property type="entry name" value="NO_sig/Golgi_transp_ligand-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002078">
        <property type="entry name" value="Sigma_54_int"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025662">
        <property type="entry name" value="Sigma_54_int_dom_ATP-bd_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025943">
        <property type="entry name" value="Sigma_54_int_dom_ATP-bd_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004096">
        <property type="entry name" value="V4R"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010523">
        <property type="entry name" value="XylR_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02954">
        <property type="entry name" value="HTH_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00158">
        <property type="entry name" value="Sigma54_activat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02830">
        <property type="entry name" value="V4R"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06505">
        <property type="entry name" value="XylR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01590">
        <property type="entry name" value="HTHFIS"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00989">
        <property type="entry name" value="V4R"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111126">
        <property type="entry name" value="SSF111126"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00675">
        <property type="entry name" value="SIGMA54_INTERACT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00676">
        <property type="entry name" value="SIGMA54_INTERACT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50045">
        <property type="entry name" value="SIGMA54_INTERACT_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Sigma-54 factor interaction" evidence="5">
        <location>
            <begin position="262"/>
            <end position="491"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50045"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABF08190.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="614" mass="67367" checksum="9B109F17CE13D6AB" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="61" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LPB1</accession>
    <name>Q1LPB1_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_1129</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08015.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515916.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1129"/>
    <dbReference type="EnsemblBacteria" id="ABF08015">
        <property type="protein sequence ID" value="ABF08015"/>
        <property type="gene ID" value="Rmet_1129"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1129"/>
    <dbReference type="eggNOG" id="COG3308">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_122357_0_1_4"/>
    <dbReference type="OMA" id="QWSSMLI"/>
    <dbReference type="OrthoDB" id="1894946at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018643">
        <property type="entry name" value="DUF2069_membrane"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09842">
        <property type="entry name" value="DUF2069"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="49"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="101"/>
            <end position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF08015.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="146" mass="16549" checksum="D8F62190246A824C" modified="2006-05-30" version="1">MNSPDTHRDGSPDALHNPALWSLSLASLITLMLLCIAWEWFLAPLRPGGSWLVLKFVLLLPVLRGVLARNRYTMQWSSMLILLYFTEGIVRATSDRAPSSWLAWGEVALTLLFFFSTILYLRPFKRRAKALTKTATKTATKTQSNH</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LPC9</accession>
    <name>Q1LPC9_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">HpcH/HpaI aldolase</fullName>
            <ecNumber evidence="2">4.1.2.20</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">hpcH</name>
        <name evidence="2" type="ordered locus">Rmet_1111</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EC" id="4.1.2.20" evidence="2"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07997.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1111"/>
    <dbReference type="EnsemblBacteria" id="ABF07997">
        <property type="protein sequence ID" value="ABF07997"/>
        <property type="gene ID" value="Rmet_1111"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1111"/>
    <dbReference type="eggNOG" id="COG3836">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_059964_4_1_4"/>
    <dbReference type="OMA" id="VAMIEDR"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008672">
        <property type="term" value="F:2-dehydro-3-deoxyglucarate aldolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005000">
        <property type="entry name" value="Aldolase/citrate-lyase_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015813">
        <property type="entry name" value="Pyrv/PenolPyrv_Kinase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040442">
        <property type="entry name" value="Pyrv_Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03328">
        <property type="entry name" value="HpcH_HpaI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51621">
        <property type="entry name" value="SSF51621"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0456">Lyase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HpcH_HpaI" evidence="1">
        <location>
            <begin position="15"/>
            <end position="237"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF07997.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="251" mass="26303" checksum="61CBF5312E9AACBE" modified="2006-05-30" version="1">MKQRLLTPGSEPIVGLFCSTPAPLTVELIAAAGYDFAVIDLEHTLIDGALLGAMLLAARASGIAPLVRVAALHQVAPALDAGAQGIVFPRITGAARASEAVAYCHHTCGIPAGKRGLNATWHSGYGRDDLCEAAEDAARHTLVVAMIEDAAGLRNADDIAAQRGVDVLLEGAADLSQSLGVPWQTRHPHVRDAVDTIAAAALRHDKHFCALPRTPADAAAWRARGTRMMVLGDDRGIARRAMAAHRQACIA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-01-15" modified="2022-05-25" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LPI4</accession>
    <name>FTSB_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Cell division protein FtsB</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">ftsB</name>
        <name type="ordered locus">Rmet_1056</name>
    </gene>
    <organism>
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Essential cell division protein. May link together the upstream cell division proteins, which are predominantly cytoplasmic, with the downstream cell division proteins, which are predominantly periplasmic.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of a complex composed of FtsB, FtsL and FtsQ.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell inner membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
        <text evidence="1">Localizes to the division septum.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the FtsB family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07942.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008650101.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q1LPI4"/>
    <dbReference type="SMR" id="Q1LPI4"/>
    <dbReference type="STRING" id="266264.Rmet_1056"/>
    <dbReference type="EnsemblBacteria" id="ABF07942">
        <property type="protein sequence ID" value="ABF07942"/>
        <property type="gene ID" value="Rmet_1056"/>
    </dbReference>
    <dbReference type="GeneID" id="60821917"/>
    <dbReference type="KEGG" id="rme:Rmet_1056"/>
    <dbReference type="eggNOG" id="COG2919">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_134863_5_0_4"/>
    <dbReference type="OMA" id="YELGMVK"/>
    <dbReference type="OrthoDB" id="2014691at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032153">
        <property type="term" value="C:cell division site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005887">
        <property type="term" value="C:integral component of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043093">
        <property type="term" value="P:FtsZ-dependent cytokinesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00599">
        <property type="entry name" value="FtsB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023081">
        <property type="entry name" value="Cell_div_FtsB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007060">
        <property type="entry name" value="FtsL/DivIC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37485">
        <property type="entry name" value="PTHR37485"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04977">
        <property type="entry name" value="DivIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0131">Cell cycle</keyword>
    <keyword id="KW-0132">Cell division</keyword>
    <keyword id="KW-0997">Cell inner membrane</keyword>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0175">Coiled coil</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_1000025718" description="Cell division protein FtsB">
        <location>
            <begin position="1"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="topological domain" description="Cytoplasmic" evidence="1">
        <location>
            <begin position="1"/>
            <end position="3"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="4"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="topological domain" description="Periplasmic" evidence="1">
        <location>
            <begin position="22"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="93"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="34"/>
            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00599"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="113" mass="12507" checksum="7CEE125146050244" modified="2006-05-30" version="1">MRLISLLLFVLLLAIQYPLWLGKGGWLRVWDLNRQVNEQTVHNQALKLRNAKLEGEVKDLQDGTGAIEERARYELGMVKDGEVFVQFVAPAPKVSATPPLPPPPNSVAGRGGH</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-01-15" modified="2022-08-03" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LPN9</accession>
    <name>HEMH_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Ferrochelatase</fullName>
            <ecNumber evidence="1">4.99.1.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Heme synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Protoheme ferro-lyase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">hemH</name>
        <name type="ordered locus">Rmet_1001</name>
    </gene>
    <organism>
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the ferrous insertion into protoporphyrin IX.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>2 H(+) + heme b = Fe(2+) + protoporphyrin IX</text>
            <dbReference type="Rhea" id="RHEA:22584"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
            <dbReference type="ChEBI" id="CHEBI:57306"/>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
            <dbReference type="EC" id="4.99.1.1"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Porphyrin-containing compound metabolism; protoheme biosynthesis; protoheme from protoporphyrin-IX: step 1/1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the ferrochelatase family.</text>
    </comment>
    <dbReference type="EC" id="4.99.1.1" evidence="1"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07887.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515801.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q1LPN9"/>
    <dbReference type="SMR" id="Q1LPN9"/>
    <dbReference type="STRING" id="266264.Rmet_1001"/>
    <dbReference type="EnsemblBacteria" id="ABF07887">
        <property type="protein sequence ID" value="ABF07887"/>
        <property type="gene ID" value="Rmet_1001"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1001"/>
    <dbReference type="eggNOG" id="COG0276">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018884_0_0_4"/>
    <dbReference type="OMA" id="LGDPYHC"/>
    <dbReference type="OrthoDB" id="780534at2"/>
    <dbReference type="UniPathway" id="UPA00252">
        <property type="reaction ID" value="UER00325"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004325">
        <property type="term" value="F:ferrochelatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006783">
        <property type="term" value="P:heme biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00419">
        <property type="entry name" value="Ferrochelatase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03411">
        <property type="entry name" value="Ferrochelatase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00323">
        <property type="entry name" value="Ferrochelatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001015">
        <property type="entry name" value="Ferrochelatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019772">
        <property type="entry name" value="Ferrochelatase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033644">
        <property type="entry name" value="Ferrochelatase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033659">
        <property type="entry name" value="Ferrochelatase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11108">
        <property type="entry name" value="PTHR11108"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00762">
        <property type="entry name" value="Ferrochelatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00109">
        <property type="entry name" value="hemH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00534">
        <property type="entry name" value="FERROCHELATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0350">Heme biosynthesis</keyword>
    <keyword id="KW-0408">Iron</keyword>
    <keyword id="KW-0456">Lyase</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0627">Porphyrin biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_1000019357" description="Ferrochelatase">
        <location>
            <begin position="1"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="218"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="299"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00323"/>
        </source>
    </evidence>
    <sequence length="371" mass="41404" checksum="F3C10F695040D69B" modified="2006-05-30" version="1">MTFSPEPAHQHGQPPRTAILLINLGTPDAPTPKAVGRYLKAFLSDPRVVEIPRLAWLPILHGLILPFRSRASAMKYESIWLREAHMTGSPLLVYTERQAHALQLLMQQQGHDVTVACAMRYGNPSIESVMEALRRQGTEQILLLPLYPQFSGTTTATAFDEAFRVLSTWRNQPELRLVKHFHDHPAYISALHKQVGGYWAQHGKPDFARGDKLILSFHGLPRRLLDLGDPYHCECLKTGRLLGEALGLQPGQFQVTFQSRFGKAEWLQPYTAPTLAELGKVGTQRVDVFCPGFPADCIETLEEIAMEGQSEFRLAGGQDFHFIPCLNDAEAWIAGLADIALQHLQGWPLNVPHPHELEARRSRAQARGAGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-10-07" version="68" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LPZ0</accession>
    <name>Q1LPZ0_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_0900</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07786.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0900"/>
    <dbReference type="EnsemblBacteria" id="ABF07786">
        <property type="protein sequence ID" value="ABF07786"/>
        <property type="gene ID" value="Rmet_0900"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0900"/>
    <dbReference type="eggNOG" id="COG3803">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_065010_2_1_4"/>
    <dbReference type="OMA" id="YMPYMHS"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010323">
        <property type="entry name" value="DUF924"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06041">
        <property type="entry name" value="DUF924"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="SSF48452"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF07786.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="200" mass="22772" checksum="01C71C473789CE0E" modified="2006-05-30" version="1">MSSDNRMPAAAQAVLDFWFGLPGAPEWNISRREWFTKCVSFDTTVRARFLSYWQAAHDGAEDDWFVTPEGACARIVLLDQFPRNMFRGDPRSFATDGKALAAARRMLDLGWGRQLPTPWHRMFCYLPFEHAESLDAQDIAVREMMALRDDSGGKVDVVEWAEKHRDVIARFGRFPHRNAVLGRVNSDAEAAYLKQPGSSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LPZ4</accession>
    <name>Q1LPZ4_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Transcription regulator protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_0896</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07782.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515722.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0896"/>
    <dbReference type="EnsemblBacteria" id="ABF07782">
        <property type="protein sequence ID" value="ABF07782"/>
        <property type="gene ID" value="Rmet_0896"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0896"/>
    <dbReference type="eggNOG" id="COG1910">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG2005">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_064037_0_0_4"/>
    <dbReference type="OMA" id="HAWGVMR"/>
    <dbReference type="OrthoDB" id="750230at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024370">
        <property type="entry name" value="PBP_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000847">
        <property type="entry name" value="Tscrpt_reg_HTH_LysR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00126">
        <property type="entry name" value="HTH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12727">
        <property type="entry name" value="PBP_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH lysR-type" evidence="1">
        <location>
            <begin position="24"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="domain" description="PBP_domain" evidence="2">
        <location>
            <begin position="135"/>
            <end position="324"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF07782.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="365" mass="40578" checksum="78EC38F57E5080AC" modified="2006-05-30" version="1">MTFRFDLFPVIAADDNPRANGKVFQLLKAVRETGSLHRAAQEIGLSYRHAWGVMRTWEEMLGRSMLDMERGRGASLTRFGERLLRAELRLRETIDPAVQKAMADFLTDLDEAARPQTVLHFSGSHDPAVETLAAALRETPAPLQLDTVFCGSVEGLVCLQERQSELAGFYVSPIQIAGSVAHVTLRKWLRPATVRLVRLAWREQGLIVAPDMAREIRDLRDLARTGARFINRQRSSGTRMLFDQLLATQGLYPDQISGYDDPEFSNEKVAEAVHSGRAQVAFGLRMNAEAHGLAFVPLTREAYYLALRKNDQTAPWVTALLSLLADPAFARRIEQLPGYTAAEPAGILTPQQALPWYGPDGKEGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="69" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LQ41</accession>
    <name>Q1LQ41_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Rick_17kDa_Anti domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_0849</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07735.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515678.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF07735">
        <property type="protein sequence ID" value="ABF07735"/>
        <property type="gene ID" value="Rmet_0849"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0849"/>
    <dbReference type="eggNOG" id="COG3133">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_090265_0_1_4"/>
    <dbReference type="OMA" id="QIEKRAG"/>
    <dbReference type="OrthoDB" id="2059597at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019867">
        <property type="term" value="C:outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008816">
        <property type="entry name" value="Gly_zipper_2TM_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05433">
        <property type="entry name" value="Rick_17kDa_Anti"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rick_17kDa_Anti" evidence="1">
        <location>
            <begin position="82"/>
            <end position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF05433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF07735.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="172" mass="17146" checksum="81D006EC16148C39" modified="2006-05-30" version="1">MQEQRKPWQVLGAAGALALVAGLAGCAQPGYGGYGNYSTAPPPTGYQQPGTNTYQAPAGSVFVGRVESIEPVQTTQGSSGILGTVIGGAAGGLLGHQIGGGSGQTVATIVGAVGGAVAGNQIEKRAGSNTSTAYRVNVRLDDGRVATVTQSNIGNLRVGDRARVANDMATAY</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="65" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LQ74</accession>
    <name>Q1LQ74_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Rmet_0816</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07702.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515647.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0816"/>
    <dbReference type="EnsemblBacteria" id="ABF07702">
        <property type="protein sequence ID" value="ABF07702"/>
        <property type="gene ID" value="Rmet_0816"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0816"/>
    <dbReference type="eggNOG" id="COG3686">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_110778_2_1_4"/>
    <dbReference type="OMA" id="YWCVLIA"/>
    <dbReference type="OrthoDB" id="2049059at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.550">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023352">
        <property type="entry name" value="MAPEG-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001129">
        <property type="entry name" value="Membr-assoc_MAPEG"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01124">
        <property type="entry name" value="MAPEG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161084">
        <property type="entry name" value="SSF161084"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="1 4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2 4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193745" evidence="5">
        <location>
            <begin position="23"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="58"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="82"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="106"/>
            <end position="126"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABF07702.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="127" mass="14206" checksum="7683E0E20561E66E" modified="2006-05-30" version="1">MPIAFWCVLVAGLMPVLTVAVAKAGSRGYDNHDPRAWLERQSGRARRADMAHRNHFEAFPFFAAAVLIASHLAAPQARIDELAIAFIVIRVLYTICYLTDRATLRTLFWTVGQLTVIGIFLLPVFVH</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LQ94</accession>
    <name>Q1LQ94_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">ABC-type transporter, permease component inner membrane subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Rmet_0796</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07682.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008645196.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0796"/>
    <dbReference type="EnsemblBacteria" id="ABF07682">
        <property type="protein sequence ID" value="ABF07682"/>
        <property type="gene ID" value="Rmet_0796"/>
    </dbReference>
    <dbReference type="GeneID" id="60825282"/>
    <dbReference type="KEGG" id="rme:Rmet_0796"/>
    <dbReference type="eggNOG" id="COG4603">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_040769_0_0_4"/>
    <dbReference type="OMA" id="FRMNLFN"/>
    <dbReference type="OrthoDB" id="974216at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001851">
        <property type="entry name" value="ABC_transp_permease"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02653">
        <property type="entry name" value="BPD_transp_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="21"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="64"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="93"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="125"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="156"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="207"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="254"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="278"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="305"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="333"/>
            <end position="353"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABF07682.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="364" mass="38652" checksum="9F81593FAAE0CF3B" modified="2006-05-30" version="1">MAKLLSPVVLAPRGVPSRRMAYASPLFAIVLTLLFGAMLFLLIGKDPMAALKVFLVDPLKDKRAIGEVLLKTVPLVLCALGLSVCYRANVWNIGADGQLILGGIFAGATVLWFDTPGNTTSGFVVLVLASLAGVVGGMVWASVTALLKDRFNANEILVSLMLTYIAQQLLLWVVNGPLKDPNGMNFPQSKVFSSAFLLPNLLPGSRLHVGFAVMLVLVVIMTVFVFRSFAGYRLQVGGTAPLAARYAGFSSRNALWSALMVSGGTAGLAGAFEVTGPIGQLLPSISPGYGFTAIIVAFVGRLHPVGAVFGGIVMSLFYIGGEMAQSRLGLPSAIGWVFQGMLLFFLLACDTLIDNRLRWRARAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="115" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LQH7</accession>
    <name>Q1LQH7_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">DNA gyrase subunit A</fullName>
            <ecNumber evidence="8">5.6.2.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8 11" type="primary">gyrA</name>
        <name evidence="11" type="ordered locus">Rmet_0713</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">A type II topoisomerase that negatively supercoils closed circular double-stranded (ds) DNA in an ATP-dependent manner to modulate DNA topology and maintain chromosomes in an underwound state. Negative supercoiling favors strand separation, and DNA replication, transcription, recombination and repair, all of which involve strand separation. Also able to catalyze the interconversion of other topological isomers of dsDNA rings, including catenanes and knotted rings. Type II topoisomerases break and join 2 DNA strands simultaneously in an ATP-dependent manner.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 8">
            <text>ATP-dependent breakage, passage and rejoining of double-stranded DNA.</text>
            <dbReference type="EC" id="5.6.2.2"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="8">Heterotetramer, composed of two GyrA and two GyrB chains. In the heterotetramer, GyrA contains the active site tyrosine that forms a transient covalent intermediate with DNA, while GyrB binds cofactors and catalyzes ATP hydrolysis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="miscellaneous">
        <text evidence="8">Few gyrases are as efficient as E.coli at forming negative supercoils. Not all organisms have 2 type II topoisomerases; in organisms with a single type II topoisomerase this enzyme also has to decatenate newly replicated chromosomes.</text>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the topoisomerase GyrA/ParC subunit family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the type II topoisomerase GyrA/ParC subunit family.</text>
    </comment>
    <dbReference type="EC" id="5.6.2.2" evidence="8"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07599.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515563.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0713"/>
    <dbReference type="EnsemblBacteria" id="ABF07599">
        <property type="protein sequence ID" value="ABF07599"/>
        <property type="gene ID" value="Rmet_0713"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0713"/>
    <dbReference type="eggNOG" id="COG0188">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002977_6_1_4"/>
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        <property type="project" value="InterPro"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
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        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034335">
        <property type="term" value="F:DNA negative supercoiling activity"/>
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        <property type="project" value="UniProt"/>
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        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
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        <property type="term" value="P:DNA-templated DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00187">
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.199.10">
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="Gyrase/topoIV_suA_C"/>
    </dbReference>
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        <property type="entry name" value="Topo_IIA-like_dom_sf"/>
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        <property type="entry name" value="Topo_IIA_A/C_ab"/>
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        <property type="entry name" value="Topo_IIA_A_a_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002205">
        <property type="entry name" value="Topo_IIA_dom_A"/>
    </dbReference>
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        <property type="match status" value="6"/>
    </dbReference>
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        <property type="entry name" value="DNA_topoisoIV"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SSF101904"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SSF56719"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7 8 11" id="KW-0413">Isomerase</keyword>
    <keyword evidence="3 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0799">Topoisomerase</keyword>
    <feature type="domain" description="TOP4c" evidence="10">
        <location>
            <begin position="11"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="863"/>
            <end position="897"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="GyrA-box" evidence="8">
        <location>
            <begin position="567"/>
            <end position="573"/>
        </location>
    </feature>
    <feature type="active site" description="O-(5'-phospho-DNA)-tyrosine intermediate" evidence="8">
        <location>
            <position position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000185"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00008263"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023029"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01897"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ABF07599.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="897" mass="99075" checksum="1D92679AA8E15507" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LQT8</accession>
    <name>Q1LQT8_CUPMC</name>
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        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_0602</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07488.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0602"/>
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        <property type="protein sequence ID" value="ABF07488"/>
        <property type="gene ID" value="Rmet_0602"/>
    </dbReference>
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        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2342064_0_0_4"/>
    <dbReference type="OMA" id="VCYVLKA"/>
    <dbReference type="OrthoDB" id="1972124at2"/>
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        <property type="component" value="Chromosome"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF07488.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="94" mass="10165" checksum="13400C50D9C0957E" modified="2006-05-30" version="1">METQESALVLALLTAPVRVTICRALLEAGEEGMSAAELAELAGLTLRHAAHHFQEMTQGEILALAIRDRRVCYTLKARVAVREALGYVDASGMN</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="100" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LQU3</accession>
    <name>Q1LQU3_CUPMC</name>
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        <recommendedName>
            <fullName evidence="2">RNA polymerase sigma factor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">rpoO</name>
        <name evidence="6" type="ordered locus">Rmet_0597</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
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            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the sigma-70 factor family. ECF subfamily.</text>
    </comment>
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        <property type="protein sequence ID" value="ABF07483.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0597"/>
    <dbReference type="EnsemblBacteria" id="ABF07483">
        <property type="protein sequence ID" value="ABF07483"/>
        <property type="gene ID" value="Rmet_0597"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0597"/>
    <dbReference type="eggNOG" id="COG1595">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047691_1_2_4"/>
    <dbReference type="OMA" id="PRIAHGA"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016987">
        <property type="term" value="F:sigma factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006352">
        <property type="term" value="P:DNA-templated transcription, initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006950">
        <property type="term" value="P:response to stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039425">
        <property type="entry name" value="RNA_pol_sigma-70-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014284">
        <property type="entry name" value="RNA_pol_sigma-70_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000838">
        <property type="entry name" value="RNA_pol_sigma70_ECF_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007627">
        <property type="entry name" value="RNA_pol_sigma70_r2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013249">
        <property type="entry name" value="RNA_pol_sigma70_r4_t2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013325">
        <property type="entry name" value="RNA_pol_sigma_r2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013324">
        <property type="entry name" value="RNA_pol_sigma_r3/r4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43133">
        <property type="entry name" value="PTHR43133"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04542">
        <property type="entry name" value="Sigma70_r2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08281">
        <property type="entry name" value="Sigma70_r4_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88659">
        <property type="entry name" value="SSF88659"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88946">
        <property type="entry name" value="SSF88946"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02937">
        <property type="entry name" value="sigma70-ECF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01063">
        <property type="entry name" value="SIGMA70_ECF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0731">Sigma factor</keyword>
    <keyword evidence="2" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Sigma70_r2" evidence="4">
        <location>
            <begin position="13"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="domain" description="Sigma70_r4_2" evidence="5">
        <location>
            <begin position="116"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="184"/>
            <end position="209"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU000716"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF04542"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF08281"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABF07483.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="209" mass="23392" checksum="F53B2C4445AFDC67" modified="2006-05-30" version="1">MDAANTRRRFDDLVLPHLDAAYNLARWLCGNPGDADDVVQEAFLRAFRLFGSFRGDQPRPWLLAIVRNTWFTTWQKRKAAAEVEYDEAAPPGELPDWHGGPGDPEQWLVRDEDVRLVHAALERLPVVFREVLVLRELEELHYRDIARIADIPVGTVMSRLARGRRMLAAAVVAVRDEGAVRLAHPDAPNTANAPADIVQPAGGTRHGLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="65" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LQX4</accession>
    <name>Q1LQX4_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Molybdenum cofactor biosynthesis protein A 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">moaA1</name>
        <name evidence="1" type="ordered locus">Rmet_0566</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07452.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515428.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0566"/>
    <dbReference type="EnsemblBacteria" id="ABF07452">
        <property type="protein sequence ID" value="ABF07452"/>
        <property type="gene ID" value="Rmet_0566"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0566"/>
    <dbReference type="HOGENOM" id="CLU_2408310_0_0_4"/>
    <dbReference type="OMA" id="PWAWVDL"/>
    <dbReference type="OrthoDB" id="2016196at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="SSF54928"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF07452.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="80" mass="9555" checksum="95CD57EF772542AD" modified="2006-05-30" version="1">MQVLIRGLHRDVTADMLREKLKIYARVISIEIMREGDADHPWAWVDLDVDAFTAWRLLRQLDKQYFAGSIMRWYIPAHQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LR26</accession>
    <name>Q1LR26_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Multidrug efflux system, subunit A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">mdtA</name>
        <name evidence="5" type="ordered locus">Rmet_0514</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07400.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0514"/>
    <dbReference type="EnsemblBacteria" id="ABF07400">
        <property type="protein sequence ID" value="ABF07400"/>
        <property type="gene ID" value="Rmet_0514"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0514"/>
    <dbReference type="eggNOG" id="COG0845">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018816_2_0_4"/>
    <dbReference type="OMA" id="DGHSRLM"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039562">
        <property type="entry name" value="MFP_biotin_lipoyl_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006143">
        <property type="entry name" value="RND_pump_MFP"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13533">
        <property type="entry name" value="Biotin_lipoyl_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01730">
        <property type="entry name" value="RND_mfp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="74"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="domain" description="Biotin_lipoyl_2" evidence="4">
        <location>
            <begin position="167"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="47"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="108"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="477"/>
            <end position="523"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF13533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABF07400.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="523" mass="54849" checksum="2B5DE12CB7F1B43C" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LRP5</accession>
    <name>Q1LRP5_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 13">Adenine DNA glycosylase</fullName>
            <ecNumber evidence="4 13">3.2.2.31</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">mutY</name>
        <name evidence="15" type="ordered locus">Rmet_0295</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Adenine glycosylase active on G-A mispairs. MutY also corrects error-prone DNA synthesis past GO lesions which are due to the oxidatively damaged form of guanine: 7,8-dihydro-8-oxoguanine (8-oxo-dGTP).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 13">
            <text>Hydrolyzes free adenine bases from 7,8-dihydro-8-oxoguanine:adenine mismatched double-stranded DNA, leaving an apurinic site.</text>
            <dbReference type="EC" id="3.2.2.31"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="13">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="13">Binds 1 [4Fe-4S] cluster.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the Nth/MutY family.</text>
    </comment>
    <dbReference type="EC" id="3.2.2.31" evidence="4 13"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07181.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0295"/>
    <dbReference type="EnsemblBacteria" id="ABF07181">
        <property type="protein sequence ID" value="ABF07181"/>
        <property type="gene ID" value="Rmet_0295"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0295"/>
    <dbReference type="eggNOG" id="COG1194">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012862_0_2_4"/>
    <dbReference type="OMA" id="RDPYRVW"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000701">
        <property type="term" value="F:purine-specific mismatch base pair DNA N-glycosylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006284">
        <property type="term" value="P:base-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd03431">
        <property type="entry name" value="DNA_Glycosylase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00056">
        <property type="entry name" value="ENDO3c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1670.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005760">
        <property type="entry name" value="A/G_AdeGlyc_MutY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011257">
        <property type="entry name" value="DNA_glycosylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003265">
        <property type="entry name" value="HhH-GPD_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023170">
        <property type="entry name" value="HhH_base_excis_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000445">
        <property type="entry name" value="HhH_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044298">
        <property type="entry name" value="MIG/MutY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029119">
        <property type="entry name" value="MutY_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015797">
        <property type="entry name" value="NUDIX_hydrolase-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42944">
        <property type="entry name" value="PTHR42944"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00633">
        <property type="entry name" value="HHH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00730">
        <property type="entry name" value="HhH-GPD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14815">
        <property type="entry name" value="NUDIX_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00478">
        <property type="entry name" value="ENDO3c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48150">
        <property type="entry name" value="SSF48150"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55811">
        <property type="entry name" value="SSF55811"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01084">
        <property type="entry name" value="mutY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="7 13" id="KW-0227">DNA damage</keyword>
    <keyword evidence="11" id="KW-0234">DNA repair</keyword>
    <keyword evidence="12 13 15" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="8 15" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9 13" id="KW-0408">Iron</keyword>
    <keyword evidence="10" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ENDO3c" evidence="14">
        <location>
            <begin position="76"/>
            <end position="233"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008343"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012045"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU365096"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM00478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="ABF07181.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="405" mass="44213" checksum="2645A8F5ED6808D0" modified="2006-05-30" version="1">MQPPMYHVAPPVSGAFSFQDPAMPRKPAAAPAMPETIAVPSDFGMRVVTWQRQHGRHDLPWQNTRDPYRIWLSEIMLQQTQVAAVIEYFQRFVTALPTVAALAAASADEVMALWAGLGYYSRARNLHRCAKTVVDEHAGVFPTDPEVLVTLPGIGRSTAAAIAAFSAGVRSPILDGNVKRVFARVFGIHGYPGERAIENRMWALAEQALPPAGRHQADHMVAYTQGLMDLGATICSRGKPACLADAEACPLVADCLARKDNLTAVLPTPKPRAAIPERSTVMLIVRHGRDVLVQLRPSRGIWGGLWSLPEMPVDTVPFDSEAAEESALELARAYGLPARAYLAGELTHVFTHFRLLIRAIRVDLDTVALEGDAASRWLSLDDLDMLGTPAPVRKLLEDQARLGLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="55" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LRS4</accession>
    <name>Q1LRS4_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_0266</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07152.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008642681.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0266"/>
    <dbReference type="EnsemblBacteria" id="ABF07152">
        <property type="protein sequence ID" value="ABF07152"/>
        <property type="gene ID" value="Rmet_0266"/>
    </dbReference>
    <dbReference type="GeneID" id="60822991"/>
    <dbReference type="KEGG" id="rme:Rmet_0266"/>
    <dbReference type="eggNOG" id="ENOG5032ZWH">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_162755_2_0_4"/>
    <dbReference type="OMA" id="RSLMFRV"/>
    <dbReference type="OrthoDB" id="2096992at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021313">
        <property type="entry name" value="DUF2909"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11137">
        <property type="entry name" value="DUF2909"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="37"/>
            <end position="59"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF07152.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="69" mass="7973" checksum="B43DE87AE51C7E1A" modified="2006-05-30" version="1">MRFVILVAFILIIGSLASALFFMMRDRGRTPNMMRSLMFRVGFSVALFLFILFSHWMGWIQSTGIHTSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LRX9</accession>
    <name>Q1LRX9_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Diadenosine tetraphosphate (Ap4A) hydrolase and other HIT family hydrolases</fullName>
            <ecNumber evidence="3">3.6.1.17</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_0211</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EC" id="3.6.1.17" evidence="3"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07097.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515116.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0211"/>
    <dbReference type="EnsemblBacteria" id="ABF07097">
        <property type="protein sequence ID" value="ABF07097"/>
        <property type="gene ID" value="Rmet_0211"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0211"/>
    <dbReference type="eggNOG" id="COG0537">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_056776_3_3_4"/>
    <dbReference type="OMA" id="IAFMDIM"/>
    <dbReference type="OrthoDB" id="1934382at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004081">
        <property type="term" value="F:bis(5'-nucleosyl)-tetraphosphatase (asymmetrical) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd01277">
        <property type="entry name" value="HINT_subgroup"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.428.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039384">
        <property type="entry name" value="HINT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001310">
        <property type="entry name" value="Histidine_triad_HIT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011146">
        <property type="entry name" value="HIT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036265">
        <property type="entry name" value="HIT-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23089">
        <property type="entry name" value="PTHR23089"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01230">
        <property type="entry name" value="HIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00332">
        <property type="entry name" value="HISTRIAD"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54197">
        <property type="entry name" value="SSF54197"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51084">
        <property type="entry name" value="HIT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HIT" evidence="2">
        <location>
            <begin position="10"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Histidine triad motif" evidence="1">
        <location>
            <begin position="102"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51084"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF07097.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="145" mass="15832" checksum="0DE17FE92A1D5D32" modified="2006-05-30" version="1">MQAEYDSNNIFARILRGELPCFKVYEDADTIAFMDIMPQSDGHTLVVPKEQAVDVFGLSEAGAAAAIRATQIVARGVREAFQPDGVVISQFNGAAAGQTVPHIHFHIVPRYVDQPLRGHARQQQDMGVLKQHAERVIAALAKLKG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2007-01-09" modified="2022-08-03" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LSB6</accession>
    <name>COAX_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Type III pantothenate kinase</fullName>
            <ecNumber evidence="1">2.7.1.33</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">PanK-III</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Pantothenic acid kinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">coaX</name>
        <name type="ordered locus">Rmet_0074</name>
    </gene>
    <organism>
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the phosphorylation of pantothenate (Pan), the first step in CoA biosynthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(R)-pantothenate + ATP = (R)-4'-phosphopantothenate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16373"/>
            <dbReference type="ChEBI" id="CHEBI:10986"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29032"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.33"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>NH4(+)</name>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
        </cofactor>
        <cofactor evidence="1">
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </cofactor>
        <text evidence="1">A monovalent cation. Ammonium or potassium.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; coenzyme A biosynthesis; CoA from (R)-pantothenate: step 1/5.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the type III pantothenate kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.33" evidence="1"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF06960.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011514998.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q1LSB6"/>
    <dbReference type="SMR" id="Q1LSB6"/>
    <dbReference type="STRING" id="266264.Rmet_0074"/>
    <dbReference type="EnsemblBacteria" id="ABF06960">
        <property type="protein sequence ID" value="ABF06960"/>
        <property type="gene ID" value="Rmet_0074"/>
    </dbReference>
    <dbReference type="GeneID" id="60822796"/>
    <dbReference type="KEGG" id="rme:Rmet_0074"/>
    <dbReference type="eggNOG" id="COG1521">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_066627_0_0_4"/>
    <dbReference type="OMA" id="NSFIKWR"/>
    <dbReference type="OrthoDB" id="2039419at2"/>
    <dbReference type="UniPathway" id="UPA00241">
        <property type="reaction ID" value="UER00352"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004594">
        <property type="term" value="F:pantothenate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015937">
        <property type="term" value="P:coenzyme A biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01274">
        <property type="entry name" value="Pantothen_kinase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004619">
        <property type="entry name" value="Type_III_PanK"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34265">
        <property type="entry name" value="PTHR34265"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03309">
        <property type="entry name" value="Pan_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="SSF53067"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0173">Coenzyme A biosynthesis</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0418">Kinase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0630">Potassium</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000270895" description="Type III pantothenate kinase">
        <location>
            <begin position="1"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="1">
        <location>
            <position position="123"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="7"/>
            <end position="14"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="114"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="121"/>
            <end position="124"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="147"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="210"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01274"/>
        </source>
    </evidence>
    <sequence length="277" mass="28670" checksum="CBA670AF419F7FAD" modified="2006-05-30" version="1">MSRLLIDIGNTRLKWAWCDDAPVAAEHVVEGGAVLPSPWRHGGAAAHAVPAERSALVGTWRQLAAGTTPEIWIANVAGPTIAAAMEAVMEDAFGAVSPHWVRTTAAHGDLVNGYREPTQLGVDRWVGSIGAHRWLPGETLLIVTAGTATTLDIVTATADGARFEGGLILPGLELMLGTLARNTAQLPELDVLDTGAGAERAVRALGADNTHDAIAAGCLAAQAGAIERSWRALAGRGPVRCLLSGGARHAVAGALAMPFEMHDNLVLLGLHAMALPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-04-26" modified="2022-08-03" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>Q58AF1</accession>
    <name>Q58AF1_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">UbiE Methyltransferase type 11</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">ubiE2</name>
        <name evidence="1" type="ordered locus">Rmet_6131</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="1 2">
        <name>pMOL30</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid pMOL30</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000354">
        <property type="protein sequence ID" value="ABF12990.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011229393.1">
        <property type="nucleotide sequence ID" value="NC_007971.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_145667.1">
        <property type="nucleotide sequence ID" value="NC_006466.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF12990">
        <property type="protein sequence ID" value="ABF12990"/>
        <property type="gene ID" value="Rmet_6131"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6131"/>
    <dbReference type="HOGENOM" id="CLU_082726_0_0_4"/>
    <dbReference type="OMA" id="QLRRHHP"/>
    <dbReference type="OrthoDB" id="1515497at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid pMOL30"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF12990.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="289" mass="32153" checksum="29D64FD369A9C03D" modified="2005-04-26" version="1">MRACIQCRAPIKHEIWSCAACGWQPMSQDGLVCMAPAMLADHDGYHEPLFEEYEKLEATHFWFVHRRRLILDVLQSHFPTLRSFMDIGCGTAENLKAIEQRFPHARICGGEASLQALASARNKSNAQFIQMDARALPFTAAFDVIGAFDVIEHVDDDKRVLAEMYRACRAGGGLILTVPQHPSLWSHLDDAAKHKRRYTYSELLGKVRAAGFQPVYATSFMSLLLPAMALSRLQQRLKPPKDAVDDGFRIGTTLNRIFGGICAAERQLILAGLRFSLGGSLLVAARKPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-01-04" modified="2022-05-25" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>Q5NV03</accession>
    <name>Q5NV03_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Type II secretory pathway, ATPase PulE/Tfp pilus assembly pathway, ATPase PilB</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">pilB</name>
        <name evidence="3" type="ordered locus">Rmet_6291</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3 4">
        <name>pMOL28</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid pMOL28</plasmid>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the GSP E family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000355">
        <property type="protein sequence ID" value="ABF13150.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011239909.1">
        <property type="nucleotide sequence ID" value="NC_007972.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_161649.1">
        <property type="nucleotide sequence ID" value="NC_006525.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF13150">
        <property type="protein sequence ID" value="ABF13150"/>
        <property type="gene ID" value="Rmet_6291"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6291"/>
    <dbReference type="HOGENOM" id="CLU_026424_0_0_4"/>
    <dbReference type="OMA" id="PERTAMM"/>
    <dbReference type="OrthoDB" id="749544at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid pMOL28"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001482">
        <property type="entry name" value="T2SS/T4SS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00437">
        <property type="entry name" value="T2SSE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="T2SP_E" evidence="2">
        <location>
            <begin position="180"/>
            <end position="457"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006611"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF13150.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="605" mass="66813" checksum="0CD3CBF1BBB81D2E" modified="2005-01-04" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2010-03-23" modified="2021-06-02" version="50" xmlns="http://uniprot.org/uniprot">
    <accession>D3DXP5</accession>
    <name>D3DXP5_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative transcriptional regulator (Helix-turn-helix motif, XRE-like)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_6450</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ADC45065.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516222.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_6450"/>
    <dbReference type="EnsemblBacteria" id="ADC45065">
        <property type="protein sequence ID" value="ADC45065"/>
        <property type="gene ID" value="Rmet_6450"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6450"/>
    <dbReference type="eggNOG" id="COG1396">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_066192_17_14_4"/>
    <dbReference type="OrthoDB" id="1933795at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00093">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001387">
        <property type="entry name" value="Cro/C1-type_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010982">
        <property type="entry name" value="Lambda_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01381">
        <property type="entry name" value="HTH_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00530">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47413">
        <property type="entry name" value="SSF47413"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50943">
        <property type="entry name" value="HTH_CROC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH cro/C1-type" evidence="1">
        <location>
            <begin position="7"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADC45065.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="101" mass="10994" checksum="19946F0B805F0585" modified="2010-03-23" version="1">MNVGQAIKLCRTRRGISQADLARQAECSVSYLSMLENNKRDPTLSTMTKIAHALHMPVGIIFFLGAEGDDLTGIGKSLQGDLARTALELLNEPVSDQRPLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-03-23" modified="2020-10-07" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>D3DXX8</accession>
    <name>D3DXX8_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_6541</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ADC45148.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_6541"/>
    <dbReference type="EnsemblBacteria" id="ADC45148">
        <property type="protein sequence ID" value="ADC45148"/>
        <property type="gene ID" value="Rmet_6541"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6541"/>
    <dbReference type="HOGENOM" id="CLU_2864649_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="39"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADC45148.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="64" mass="6634" checksum="45A3E131428EDA0E" modified="2010-03-23" version="1">MVGVVACVPVLEVVALVGVESPPPPQPANAMVRQAKDTAVSFAGYVIRLPLVLISLYALFDGSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-03-23" modified="2020-02-26" version="22" xmlns="http://uniprot.org/uniprot">
    <accession>D3DYD4</accession>
    <name>D3DYD4_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_6725</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ADC45304.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADC45304">
        <property type="protein sequence ID" value="ADC45304"/>
        <property type="gene ID" value="Rmet_6725"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6725"/>
    <dbReference type="HOGENOM" id="CLU_3428312_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADC45304.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="20" mass="2203" checksum="7EFBDF10584A1DF7" modified="2010-03-23" version="1">MLANVLRPGPADFFALWLNG</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-03-23" modified="2020-10-07" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>D3DYG0</accession>
    <name>D3DYG0_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_6751</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ADC45330.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_6751"/>
    <dbReference type="EnsemblBacteria" id="ADC45330">
        <property type="protein sequence ID" value="ADC45330"/>
        <property type="gene ID" value="Rmet_6751"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6751"/>
    <dbReference type="HOGENOM" id="CLU_2846696_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADC45330.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="65" mass="6785" checksum="CE4F08E967D69B27" modified="2010-03-23" version="1">MEQGGATRAGGLNVLVIDNGRAKCGRELIHSHSLMQSCDPEGSRLDICGTELPRLVAASPRAPGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-10-07" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LAE6</accession>
    <name>Q1LAE6_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_6021</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="1 2">
        <name>pMOL30</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid pMOL30</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000354">
        <property type="protein sequence ID" value="ABF12880.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF12880">
        <property type="protein sequence ID" value="ABF12880"/>
        <property type="gene ID" value="Rmet_6021"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6021"/>
    <dbReference type="HOGENOM" id="CLU_1546358_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid pMOL30"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF12880.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="173" mass="18495" checksum="1749F0172F39CE1F" modified="2006-05-30" version="1">MDTQCLDDVAAAGEPAGRSEQYLVGWRFGDWYLQQGGDPDGRASELWSAEKASGFLDRLALRVESPKRGAAGGSAQAEPGRPDAEVSAFEVLTSAHGDSVWVNWADGSCIARFSKRFGIDVHAGGEAQIAGAPQCLFCAHGVAGLEQWHTFRQKVREHHGIDVPNDAIAFAGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="65" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LAX1</accession>
    <name>Q1LAX1_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_5846</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12705.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518054.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5846"/>
    <dbReference type="EnsemblBacteria" id="ABF12705">
        <property type="protein sequence ID" value="ABF12705"/>
        <property type="gene ID" value="Rmet_5846"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5846"/>
    <dbReference type="eggNOG" id="ENOG5033WFE">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_507939_0_0_4"/>
    <dbReference type="OrthoDB" id="622243at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.20.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012334">
        <property type="entry name" value="Pectin_lyas_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011050">
        <property type="entry name" value="Pectin_lyase_fold/virulence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006311">
        <property type="entry name" value="TAT_signal"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51126">
        <property type="entry name" value="SSF51126"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51318">
        <property type="entry name" value="TAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193254" evidence="1">
        <location>
            <begin position="24"/>
            <end position="536"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF12705.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="536" mass="57842" checksum="57EA66D11ABDF02F" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="98" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LAY6</accession>
    <name>Q1LAY6_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Transcriptional regulator, LuxR family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_5831</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12690.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518040.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5831"/>
    <dbReference type="EnsemblBacteria" id="ABF12690">
        <property type="protein sequence ID" value="ABF12690"/>
        <property type="gene ID" value="Rmet_5831"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5831"/>
    <dbReference type="eggNOG" id="COG2197">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_90_8_4"/>
    <dbReference type="OMA" id="HATAYET"/>
    <dbReference type="OrthoDB" id="1687028at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06170">
        <property type="entry name" value="LuxR_C_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016032">
        <property type="entry name" value="Sig_transdc_resp-reg_C-effctor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000792">
        <property type="entry name" value="Tscrpt_reg_LuxR_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00196">
        <property type="entry name" value="GerE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00038">
        <property type="entry name" value="HTHLUXR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00421">
        <property type="entry name" value="HTH_LUXR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46894">
        <property type="entry name" value="SSF46894"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50043">
        <property type="entry name" value="HTH_LUXR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH luxR-type" evidence="2">
        <location>
            <begin position="181"/>
            <end position="246"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF12690.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="276" mass="29317" checksum="8CD4C5D1920B0C89" modified="2006-05-30" version="1">MATILLFEPHPLLRLGLHHLLSQAHVPGDLVDLDPTTLHAPEPWVHHADLLIIGMPADSGAGWPILAELCLRLQPLRVLVLADHLPAPMPEGGLPEKVRGLLAKTCSSEALEAAIRLVLAGGECFPSHAPANRVHEPLADAVPAELSRLVATPVVVPLHGADAAAPAHADTHAVPTETPAVGAHLLNITERQYEVLALLARGYPIKTVSRLLNISVATAKTHACTLYQRLHVRNKGEAVYVALQRGASLNWPGPTPARPTHIPMSSLSAQHACEAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LBE6</accession>
    <name>Q1LBE6_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Copper resistance protein A, multi-copper oxidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">copA2</name>
        <name evidence="7" type="ordered locus">Rmet_5671</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="8">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="8">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Periplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12530.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011520076.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="SMR" id="Q1LBE6"/>
    <dbReference type="STRING" id="266264.Rmet_5671"/>
    <dbReference type="EnsemblBacteria" id="ABF12530">
        <property type="protein sequence ID" value="ABF12530"/>
        <property type="gene ID" value="Rmet_5671"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5671"/>
    <dbReference type="eggNOG" id="COG2132">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009100_5_2_4"/>
    <dbReference type="OMA" id="WNQMRMS"/>
    <dbReference type="OrthoDB" id="971126at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042597">
        <property type="term" value="C:periplasmic space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd13848">
        <property type="entry name" value="CuRO_1_CopA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13874">
        <property type="entry name" value="CuRO_2_CopA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13896">
        <property type="entry name" value="CuRO_3_CopA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.420">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001117">
        <property type="entry name" value="Cu-oxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011706">
        <property type="entry name" value="Cu-oxidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045087">
        <property type="entry name" value="Cu-oxidase_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011707">
        <property type="entry name" value="Cu-oxidase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006376">
        <property type="entry name" value="Cu-R_CopA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033138">
        <property type="entry name" value="Cu_oxidase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002355">
        <property type="entry name" value="Cu_oxidase_Cu_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008972">
        <property type="entry name" value="Cupredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034284">
        <property type="entry name" value="CuRO_1_CopA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034282">
        <property type="entry name" value="CuRO_2_CopA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034279">
        <property type="entry name" value="CuRO_3_CopA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006311">
        <property type="entry name" value="TAT_signal"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019546">
        <property type="entry name" value="TAT_signal_bac_arc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11709">
        <property type="entry name" value="PTHR11709"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00394">
        <property type="entry name" value="Cu-oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07731">
        <property type="entry name" value="Cu-oxidase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07732">
        <property type="entry name" value="Cu-oxidase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49503">
        <property type="entry name" value="SSF49503"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01480">
        <property type="entry name" value="copper_res_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01409">
        <property type="entry name" value="TAT_signal_seq"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00079">
        <property type="entry name" value="MULTICOPPER_OXIDASE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00080">
        <property type="entry name" value="MULTICOPPER_OXIDASE2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51318">
        <property type="entry name" value="TAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-0614">Plasmid</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Plastocyanin-like" evidence="6">
        <location>
            <begin position="72"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="domain" description="Plastocyanin-like" evidence="4">
        <location>
            <begin position="190"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="domain" description="Plastocyanin-like" evidence="5">
        <location>
            <begin position="491"/>
            <end position="604"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF07731"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF07732"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABF12530.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="605" mass="66594" checksum="E245910490B4DA1C" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="100" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LBJ4</accession>
    <name>Q1LBJ4_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Peptidyl-prolyl cis-trans isomerase</fullName>
            <ecNumber evidence="4">5.2.1.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Rmet_5623</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="8">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="8">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 3">
            <text>[protein]-peptidylproline (omega=180) = [protein]-peptidylproline (omega=0)</text>
            <dbReference type="Rhea" id="RHEA:16237"/>
            <dbReference type="Rhea" id="RHEA-COMP:10747"/>
            <dbReference type="Rhea" id="RHEA-COMP:10748"/>
            <dbReference type="ChEBI" id="CHEBI:83833"/>
            <dbReference type="ChEBI" id="CHEBI:83834"/>
            <dbReference type="EC" id="5.2.1.8"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the FKBP-type PPIase family.</text>
    </comment>
    <dbReference type="EC" id="5.2.1.8" evidence="4"/>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12482.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008643156.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5623"/>
    <dbReference type="EnsemblBacteria" id="ABF12482">
        <property type="protein sequence ID" value="ABF12482"/>
        <property type="gene ID" value="Rmet_5623"/>
    </dbReference>
    <dbReference type="GeneID" id="60824438"/>
    <dbReference type="KEGG" id="rme:Rmet_5623"/>
    <dbReference type="eggNOG" id="COG0545">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013615_7_4_4"/>
    <dbReference type="OMA" id="FTSMNNQ"/>
    <dbReference type="OrthoDB" id="1861282at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003755">
        <property type="term" value="F:peptidyl-prolyl cis-trans isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.50.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046357">
        <property type="entry name" value="PPIase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001179">
        <property type="entry name" value="PPIase_FKBP_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00254">
        <property type="entry name" value="FKBP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50059">
        <property type="entry name" value="FKBP_PPIASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4 7" id="KW-0413">Isomerase</keyword>
    <keyword evidence="7" id="KW-0614">Plasmid</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3 4" id="KW-0697">Rotamase</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193278" description="Peptidyl-prolyl cis-trans isomerase" evidence="5">
        <location>
            <begin position="22"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="domain" description="PPIase FKBP-type" evidence="6">
        <location>
            <begin position="44"/>
            <end position="130"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003915"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABF12482.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="131" mass="13607" checksum="12C91B7DF601EB08" modified="2006-05-30" version="1">MKKLSLILAAVALGAAGIAQAETLPSGVVIEHVTKGTGASPKATDTVKVHYRGTLTDGKEFDSSYKRGQPISFPLNRVIPCWTEGVQKMQVGGKAKLTCPASTAYGERGVPGTIPPNATLNFEVELLGIGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="97" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LBX0</accession>
    <name>Q1LBX0_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Transposase catalytic site ISRme11</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">tnpB</name>
        <name evidence="2" type="ordered locus">Rmet_5455</name>
        <name evidence="3" type="ordered locus">Rmet_5497</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid</name>
    </geneLocation>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="submission" date="2006-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of the chromosome of Ralstonia metallidurans CH34.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Copeland A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Barry K."/>
                <person name="Detter J.C."/>
                <person name="Glavina del Rio T."/>
                <person name="Hammon N."/>
                <person name="Israni S."/>
                <person name="Dalin E."/>
                <person name="Tice H."/>
                <person name="Martinez M."/>
                <person name="Goltsman E."/>
                <person name="Pitluck S."/>
                <person name="Schmutz J."/>
                <person name="Larimer F."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Kim E."/>
                <person name="Mergeay M."/>
                <person name="Benotmane M.A."/>
                <person name="Vallaeys T."/>
                <person name="Michaux A."/>
                <person name="Monchy S."/>
                <person name="Dunn J."/>
                <person name="McCorkle S."/>
                <person name="Taghavi S."/>
                <person name="van der Lelie D."/>
                <person name="Richardson P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">CH34</strain>
            <plasmid evidence="3">megaplasmid</plasmid>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2010-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Benotmane R."/>
                <person name="Lapidus A."/>
                <person name="McCorkle S."/>
                <person name="Monchy S."/>
                <person name="Taghavi S."/>
                <person name="van der Lelie N."/>
                <person name="Dunn J."/>
                <person name="Leys N."/>
                <person name="Mergeay M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">CH34</strain>
            <plasmid evidence="3">megaplasmid</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12314.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12356.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5455"/>
    <dbReference type="EnsemblBacteria" id="ABF12314">
        <property type="protein sequence ID" value="ABF12314"/>
        <property type="gene ID" value="Rmet_5455"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF12356">
        <property type="protein sequence ID" value="ABF12356"/>
        <property type="gene ID" value="Rmet_5497"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5455"/>
    <dbReference type="KEGG" id="rme:Rmet_5497"/>
    <dbReference type="eggNOG" id="COG2801">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027402_4_2_4"/>
    <dbReference type="OMA" id="SHFKAEC"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032196">
        <property type="term" value="P:transposition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025948">
        <property type="entry name" value="HTH-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038965">
        <property type="entry name" value="InsF-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001584">
        <property type="entry name" value="Integrase_cat-core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46889:SF1">
        <property type="entry name" value="PTHR46889:SF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13276">
        <property type="entry name" value="HTH_21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00665">
        <property type="entry name" value="rve"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13333">
        <property type="entry name" value="rve_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="SSF53098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50994">
        <property type="entry name" value="INTEGRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Integrase catalytic" evidence="1">
        <location>
            <begin position="116"/>
            <end position="279"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF12314.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF12356.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="283" mass="32452" checksum="3D59487470372DFE" modified="2006-05-30" version="1">MRSHISEYCLRTMCRVLGVHRSGYYVWQRQASSPRRREDDRLLGLIKHHWLASGGVYGYRKITVDLRESGEACSRHRVHRLMKGEGLRAQVGYGSKPRYRGGPVGVVANVLNREFAPDAPNKVWVTDITYIRTYEGWLYLAAVMDLYSRQIVGWATRSTMTSDLVLQALLAAVWKRKPRAGVMVHSDQGSQFTSDDWQSFLKAHNMVPSMSRRGNCHDNAVAESFFSALKKERIKRRIYPNRATAATDVFDYIEMFYNPIRRHGSAGDLSPVEFERRYALNGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="63" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LC83</accession>
    <name>Q1LC83_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_5384</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12243.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008648313.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5384"/>
    <dbReference type="EnsemblBacteria" id="ABF12243">
        <property type="protein sequence ID" value="ABF12243"/>
        <property type="gene ID" value="Rmet_5384"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5384"/>
    <dbReference type="eggNOG" id="COG3071">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_181383_0_1_4"/>
    <dbReference type="OMA" id="FYFPITT"/>
    <dbReference type="OrthoDB" id="2032133at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021320">
        <property type="entry name" value="DUF2905"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36443">
        <property type="entry name" value="PTHR36443"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11146">
        <property type="entry name" value="DUF2905"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="42"/>
            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF12243.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="66" mass="7610" checksum="2DC4C756119F6D34" modified="2006-05-30" version="1">MQRLLLILGCLFLVAAIAWPWLSRLPLGRLPGDIRIVRDGFSFYFPITTCILISLLVSLVLWLLRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LCA3</accession>
    <name>Q1LCA3_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">2,5-diketo-D-gluconate reductase B</fullName>
            <ecNumber evidence="5">1.1.1.274</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">dkgB</name>
        <name evidence="5" type="ordered locus">Rmet_5364</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="6">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="6">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EC" id="1.1.1.274" evidence="5"/>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12223.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519769.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5364"/>
    <dbReference type="EnsemblBacteria" id="ABF12223">
        <property type="protein sequence ID" value="ABF12223"/>
        <property type="gene ID" value="Rmet_5364"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5364"/>
    <dbReference type="eggNOG" id="COG0656">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023205_0_1_4"/>
    <dbReference type="OMA" id="WRHPDEP"/>
    <dbReference type="OrthoDB" id="1035565at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050580">
        <property type="term" value="F:2,5-didehydrogluconate reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020471">
        <property type="entry name" value="AKR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018170">
        <property type="entry name" value="Aldo/ket_reductase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023210">
        <property type="entry name" value="NADP_OxRdtase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036812">
        <property type="entry name" value="NADP_OxRdtase_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43827">
        <property type="entry name" value="PTHR43827"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00248">
        <property type="entry name" value="Aldo_ket_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000097">
        <property type="entry name" value="AKR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00069">
        <property type="entry name" value="ALDKETRDTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51430">
        <property type="entry name" value="SSF51430"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00798">
        <property type="entry name" value="ALDOKETO_REDUCTASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00062">
        <property type="entry name" value="ALDOKETO_REDUCTASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-0614">Plasmid</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aldo_ket_red" evidence="4">
        <location>
            <begin position="17"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="1">
        <location>
            <position position="39"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="97"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Lowers pKa of active site Tyr" evidence="3">
        <location>
            <position position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRSR" id="PIRSR000097-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR000097-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR000097-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABF12223.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="267" mass="28784" checksum="070110A07FDC9993" modified="2006-05-30" version="1">MSIPSFGVGTFRLTGQVVVDSVRNALDVGYRAIDTAQIYGNEADVGQAIAESGVERGELFVTTKIWTANYAADKLVPSLRESLDKLRTDYVDLTLIHWPAPGNGVALSEYMAALAEAKALGLTRQIGVSNFNIELTRQAIEAVGKGEIATNQIELSPYLQSRKLTAFLKEQGIAVTSYMTLAYGKVLKDPVLGQIAAKHHATVAQVALAWALQLGYAVIPSSTKRENLASNLLARDLKLDADDMALIASLERNGREVSPEGLAPVWD</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="67" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LCG0</accession>
    <name>Q1LCG0_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative Heme/copper membrane protein, NnrS family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_5307</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12166.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519712.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5307"/>
    <dbReference type="EnsemblBacteria" id="ABF12166">
        <property type="protein sequence ID" value="ABF12166"/>
        <property type="gene ID" value="Rmet_5307"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5307"/>
    <dbReference type="eggNOG" id="COG3213">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041785_2_0_4"/>
    <dbReference type="OMA" id="MLAWHRH"/>
    <dbReference type="OrthoDB" id="791105at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010266">
        <property type="entry name" value="NnrS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05940">
        <property type="entry name" value="NnrS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="30"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="64"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="99"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="122"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="152"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="179"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="221"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="245"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="274"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="305"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="342"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="368"/>
            <end position="385"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF12166.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="401" mass="42668" checksum="488027D343E3627C" modified="2006-05-30" version="1">MSVLLQISPPSRSQAGYRGLPVLALGFRPFYLLAAAFGALAVVAWVAMYLGWWTPASQPWAGTMVWHAHEMVFGFSAAVVVGFLFTAGKNWTGLQTPQGAWLGALVGVWLLARVLMWSGPAWAAITVDVLFLPLCSLSFYRILRRAKSQRNYGLAIALGVMGAFNIGFHAAVLSGRIDWALHASEAAIGLVAIFVTVIGGRVIPMFTANAIPGIRIRRWAWVERCVIPLTLLAALASAVLAPAIILAPLALAAAIAQGVRLAGWDSLRTVRKPIVSILHAAYAFLPIGFLLIAAASLGWVDRSTALHALTVGVIGGAIIAMVTRTALGHSGRRLETGRMEHLAYLCMALAAIARVAGPIALPALKPEAIAASGVLWALSLVLYLVKYTPYLTRARIDGKEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="96" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LCI2</accession>
    <name>Q1LCI2_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Quinone oxidoreductase, NADPH-dependent</fullName>
            <ecNumber evidence="2">1.6.5.5</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">qor</name>
        <name evidence="2" type="ordered locus">Rmet_5285</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EC" id="1.6.5.5" evidence="2"/>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12144.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519690.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5285"/>
    <dbReference type="EnsemblBacteria" id="ABF12144">
        <property type="protein sequence ID" value="ABF12144"/>
        <property type="gene ID" value="Rmet_5285"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5285"/>
    <dbReference type="eggNOG" id="COG0604">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026673_3_1_4"/>
    <dbReference type="OMA" id="CTANWIA"/>
    <dbReference type="OrthoDB" id="831053at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003960">
        <property type="term" value="F:NADPH:quinone reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013149">
        <property type="entry name" value="ADH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013154">
        <property type="entry name" value="ADH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020843">
        <property type="entry name" value="PKS_ER"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002364">
        <property type="entry name" value="Quin_OxRdtase/zeta-crystal_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08240">
        <property type="entry name" value="ADH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00107">
        <property type="entry name" value="ADH_zinc_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00829">
        <property type="entry name" value="PKS_ER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="SSF50129"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01162">
        <property type="entry name" value="QOR_ZETA_CRYSTAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PKS_ER" evidence="1">
        <location>
            <begin position="11"/>
            <end position="324"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF12144.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="326" mass="34557" checksum="095506A9D66E1C8E" modified="2006-05-30" version="1">MAKVVRIYETGGPDVLRFENATVGEPGPGEVRVRHVAVGLNFADTYFRSGLYKVPLPSSIGNEAAGVIEAVGSGVTHLAVGDRVTYTGFTPTLGAYSTDRLISADPLIKLPEAISCEVAAAMTMRGLSAAYLMRRIYPFKAGDTILLHAAAGGAGLIISQWAKLLGLTVIGTVSTDAKAEVARAHGCDHTINYSHEDVAKRVRELTDGVGVSVVFDSVGKDTFMSSLDSLKRRGLLVCVGFASGAVPAFDPAILARKGSAYLTRPGLADYIADPAEKAELVDELFGHVAAGRIRIDINRRYALEDAVQAHRDLEARKIVGSSIFAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="111" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LD26</accession>
    <name>Q1LD26_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Oligopeptide/dipeptide ABC transporter (ATP-binding)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">dppD</name>
        <name evidence="10" type="ordered locus">Rmet_5091</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="11">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="11">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11950.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519496.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5091"/>
    <dbReference type="EnsemblBacteria" id="ABF11950">
        <property type="protein sequence ID" value="ABF11950"/>
        <property type="gene ID" value="Rmet_5091"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5091"/>
    <dbReference type="eggNOG" id="COG0444">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_23_4"/>
    <dbReference type="OMA" id="QMTEVII"/>
    <dbReference type="OrthoDB" id="1101128at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015833">
        <property type="term" value="P:peptide transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013563">
        <property type="entry name" value="Oligopep_ABC_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08352">
        <property type="entry name" value="oligo_HPY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01727">
        <property type="entry name" value="oligo_HPY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 8 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-0614">Plasmid</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-1278">Translocase</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ABC transporter" evidence="9">
        <location>
            <begin position="15"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="51"/>
            <end position="58"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ABF11950.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="348" mass="37570" checksum="398D30E86278AD8E" modified="2006-05-30" version="1">MTSTRKALPAGTPLLEVQDLHVRFDTLTGPARSVNGVSYTVRAGQTLGVVGESGCGKSVTALSIMRLLSAPPAHVTGAVLLDGTDLLTLGEREMRRIRGNRISMIFQEPMTSLNPVLTIGRQIAETVRLHQGASHAEALARAIDMLRLVQIPEPERRANEYPHQLSGGMRQRVMIALALACNPEVLIADEPTTALDVTIQAQILDLIRRLQQELGTGVVMITHDLGVVAESCHRVVVMYAGRKVEEANVADLFDRPLHPYTRALMASMPSMNTGSSRLTEIPGLVPSPQQPQRGCAFAPRCAQAQARCHTEMPRLSAQGADHFVACFAVETRQSDGVAPVDSTPETLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LD36</accession>
    <name>Q1LD36_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Rmet_5081</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="6">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="6">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11940.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519487.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5081"/>
    <dbReference type="EnsemblBacteria" id="ABF11940">
        <property type="protein sequence ID" value="ABF11940"/>
        <property type="gene ID" value="Rmet_5081"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5081"/>
    <dbReference type="eggNOG" id="COG1511">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016648_0_0_4"/>
    <dbReference type="OMA" id="TKLGDEW"/>
    <dbReference type="OrthoDB" id="226396at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008520">
        <property type="entry name" value="DUF802"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05650">
        <property type="entry name" value="DUF802"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0614">Plasmid</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="7"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="33"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="112"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="domain" description="DUF802" evidence="4">
        <location>
            <begin position="325"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="domain" description="DUF802" evidence="4">
        <location>
            <begin position="381"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="721"/>
            <end position="742"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="439"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="721"/>
            <end position="741"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF05650"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABF11940.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="1031" mass="110654" checksum="66FFA013125DF4BE" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LD52</accession>
    <name>Q1LD52_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Hydroxyquinol 1,2-dioxygenase</fullName>
            <ecNumber evidence="8">1.13.11.37</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Rmet_5062</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="9">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="9">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Fe(3+)</name>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the intradiol ring-cleavage dioxygenase family.</text>
    </comment>
    <dbReference type="EC" id="1.13.11.37" evidence="8"/>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11924.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519474.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5062"/>
    <dbReference type="EnsemblBacteria" id="ABF11924">
        <property type="protein sequence ID" value="ABF11924"/>
        <property type="gene ID" value="Rmet_5062"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5062"/>
    <dbReference type="eggNOG" id="COG3485">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046727_1_1_4"/>
    <dbReference type="OMA" id="PWHLMTY"/>
    <dbReference type="OrthoDB" id="1456634at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047074">
        <property type="term" value="F:4-hydroxycatechol 1,2-dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018576">
        <property type="term" value="F:catechol 1,2-dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008199">
        <property type="term" value="F:ferric iron binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018581">
        <property type="term" value="F:hydroxyquinol 1,2-dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009712">
        <property type="term" value="P:catechol-containing compound metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03461">
        <property type="entry name" value="1_2-HQD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.130.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039390">
        <property type="entry name" value="1_2-HQD/HQD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007535">
        <property type="entry name" value="Catechol_dOase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000627">
        <property type="entry name" value="Intradiol_dOase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015889">
        <property type="entry name" value="Intradiol_dOase_core"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00775">
        <property type="entry name" value="Dioxygenase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04444">
        <property type="entry name" value="Dioxygenase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49482">
        <property type="entry name" value="SSF49482"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00083">
        <property type="entry name" value="INTRADIOL_DIOXYGENAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="6" id="KW-0408">Iron</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-0614">Plasmid</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="INTRADIOL_DIOXYGENAS" evidence="7">
        <location>
            <begin position="133"/>
            <end position="161"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001965"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS00083"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ABF11924.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="296" mass="32633" checksum="03F16151633BC346" modified="2006-05-30" version="1">MEATPHAAAEAALTEQVVARFADTPDPRLRALMQGLVRHLHAYVRECEPTEAEWFAAIQFLTDTGKMCDGLVRQEFILLSDTLGVSMLVDAINHRQSTGATETTVFGPFYIAGMPDRSAGENMALTPGTPALVHGRVFSADGTPLRGAVLDIWQTAENGMYSGQDTAQPTGNLRGRYRTDDEGDYAIRTIVPVSYPIPTDGPVGQMLTSTRRHPWRPAHLHFMIDASGHRRLVTHLFNQGDPYLDSDAVFGVKPSLQVAYEERPADHPLARRFGFDGPFREARYDFVLDPVPKAGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="72" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LDF2</accession>
    <name>Q1LDF2_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative diguanylate phosphodiesterase (EAL domain)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_4962</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11824.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4962"/>
    <dbReference type="EnsemblBacteria" id="ABF11824">
        <property type="protein sequence ID" value="ABF11824"/>
        <property type="gene ID" value="Rmet_4962"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4962"/>
    <dbReference type="eggNOG" id="COG5001">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_70_50_4"/>
    <dbReference type="OMA" id="NEMHELY"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="CDD" id="cd01948">
        <property type="entry name" value="EAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.450">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001633">
        <property type="entry name" value="EAL_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035919">
        <property type="entry name" value="EAL_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00563">
        <property type="entry name" value="EAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00052">
        <property type="entry name" value="EAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF141868">
        <property type="entry name" value="SSF141868"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50883">
        <property type="entry name" value="EAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="EAL" evidence="2">
        <location>
            <begin position="36"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF11824.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="298" mass="33178" checksum="F0859A9268BF4131" modified="2006-05-30" version="1">MSWMQEMHARPRESGKSECPQMQVAEDTMESWWAPELLQVEVLRNAIDCGEFVACYQPQFDLDGGHLRGVEALARWARPGFGMIPPAAFLGALREHDLLSELTIVMIWHAAAAVRRWEKGGLALPISINAEWRCLETGRLHVALRSACQHFGISMRSLHVEVSEQTEVNDLMTARDAIRALRAEGVSIALDDFGTGTTSLQLLRHIECDSIKLDQSLVKNIGRDPRAASLLGSVIGMVRAMGLKAIAEGVENFDDLECLIRLGCHQAQGFLLGAPMPEARLAHQFFKRQVSARWKPEM</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LDG5</accession>
    <name>Q1LDG5_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Acyl-CoA synthetase</fullName>
            <ecNumber evidence="3">6.2.1.26</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_4949</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EC" id="6.2.1.26" evidence="3"/>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11811.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519370.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4949"/>
    <dbReference type="EnsemblBacteria" id="ABF11811">
        <property type="protein sequence ID" value="ABF11811"/>
        <property type="gene ID" value="Rmet_4949"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4949"/>
    <dbReference type="eggNOG" id="COG0318">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000022_59_0_4"/>
    <dbReference type="OMA" id="FYNCFGQ"/>
    <dbReference type="OrthoDB" id="377638at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008756">
        <property type="term" value="F:o-succinylbenzoate-CoA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025110">
        <property type="entry name" value="AMP-bd_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045851">
        <property type="entry name" value="AMP-bd_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020845">
        <property type="entry name" value="AMP-binding_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_Synth/Lig"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13193">
        <property type="entry name" value="AMP-binding_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00455">
        <property type="entry name" value="AMP_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0436">Ligase</keyword>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AMP-binding" evidence="1">
        <location>
            <begin position="19"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="domain" description="AMP-binding_C" evidence="2">
        <location>
            <begin position="427"/>
            <end position="502"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF11811.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="521" mass="56608" checksum="8F7788815300A038" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LDI7</accession>
    <name>Q1LDI7_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Tyrosine aminotransferase, tyrosine-repressible, PLP-dependent</fullName>
            <ecNumber evidence="5">2.6.1.57</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">tyrB</name>
        <name evidence="5" type="ordered locus">Rmet_4927</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="6">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="6">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.6.1.57" evidence="5"/>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11789.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519349.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4927"/>
    <dbReference type="EnsemblBacteria" id="ABF11789">
        <property type="protein sequence ID" value="ABF11789"/>
        <property type="gene ID" value="Rmet_4927"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4927"/>
    <dbReference type="eggNOG" id="COG1448">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032440_1_2_4"/>
    <dbReference type="OMA" id="WDQNKRQ"/>
    <dbReference type="OrthoDB" id="417859at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080130">
        <property type="term" value="F:L-phenylalanine:2-oxoglutarate aminotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009058">
        <property type="term" value="P:biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006520">
        <property type="term" value="P:cellular amino acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000796">
        <property type="entry name" value="Asp_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11879">
        <property type="entry name" value="PTHR11879"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00799">
        <property type="entry name" value="TRANSAMINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="SSF53383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="5" id="KW-0614">Plasmid</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aminotran_1_2" evidence="4">
        <location>
            <begin position="26"/>
            <end position="392"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABF11789.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="398" mass="43770" checksum="92C614297898AB5F" modified="2006-05-30" version="1">MFEHIEAFPGDPILSLNEDFQQDPRTNKVNLSIGIYFDDEGRLPVMKAVAEAEAALLSDMGPRPYLPMSGMAAYRQAVQALVFGEDCAARAEGRVATLQTLGGSGALRVGADFLKRYFPKAEMWISDPSWENHRVVFERAGFKVNTYPYYDPATGGLKFDAMLAAIEAIPQGDIVLLHACCHNPTGVDLNQDQWRQVIGVLKARKLLPFVDMAYQGFGSGLDDDAFAVRELARQNVPCLVANSFSKNFSLYGERCGGLSVVCQSADEASRVLGQLTGAVRANYSNPPTHGARVVARVLTTPALRANWEQELAAMCQRITRMRQAIHDHLREHVSGEKLSRYITQRGMFTYTGLSADQVDRLKNEHGVYVLRSGRMCVAGLNERNVGVVANAIGQVLKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LDQ5</accession>
    <name>Q1LDQ5_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative NADH-depentdent flavin oxidoreductase</fullName>
            <ecNumber evidence="2">1.6.99.1</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_4859</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2017" name="Proteins" volume="85" first="2252" last="2257">
            <title>Structural investigation into the C-terminal extension of the ene-reductase from Ralstonia (Cupriavidus) metallidurans.</title>
            <authorList>
                <person name="Opperman D.J."/>
            </authorList>
            <dbReference type="PubMed" id="28833623"/>
            <dbReference type="DOI" id="10.1002/prot.25372"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (1.70 ANGSTROMS)</scope>
    </reference>
    <dbReference type="EC" id="1.6.99.1" evidence="2"/>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11721.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519282.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="PDB" id="5OCS">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="1.70 A"/>
        <property type="chains" value="A/B/C/D=1-371"/>
    </dbReference>
    <dbReference type="PDBsum" id="5OCS"/>
    <dbReference type="SMR" id="Q1LDQ5"/>
    <dbReference type="STRING" id="266264.Rmet_4859"/>
    <dbReference type="EnsemblBacteria" id="ABF11721">
        <property type="protein sequence ID" value="ABF11721"/>
        <property type="gene ID" value="Rmet_4859"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4859"/>
    <dbReference type="eggNOG" id="COG1902">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012153_2_0_4"/>
    <dbReference type="OMA" id="NQRRDQY"/>
    <dbReference type="OrthoDB" id="1033671at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003959">
        <property type="term" value="F:NADPH dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018548">
        <property type="term" value="F:pentaerythritol trinitrate reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd02932">
        <property type="entry name" value="OYE_YqiM_FMN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001155">
        <property type="entry name" value="OxRdtase_FMN_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044152">
        <property type="entry name" value="YqjM-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43303">
        <property type="entry name" value="PTHR43303"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00724">
        <property type="entry name" value="Oxidored_FMN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword evidence="4" id="KW-0002">3D-structure</keyword>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Oxidored_FMN" evidence="1">
        <location>
            <begin position="4"/>
            <end position="339"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00724"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF11721.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="4">
        <source>
            <dbReference type="PDB" id="5OCS"/>
        </source>
    </evidence>
    <sequence length="371" mass="40142" checksum="4F0EEE245DD46758" modified="2006-05-30" version="1">MPHLFDPYRIGNLELANRIAIAPMCQYSAQEGNATDWHMIHLGQMALSGAGLLIIEATAVSPEGRITPTDLGLYNDANEAALGRVLGAVRNHSPIAVTIQLAHAGRKASSEAPWDGGGQIRPDQPRGWQTFAPSAVPHAAGEVPPAALDKAGMKKIRDDFVAAAKRAARLGIEGIEVHGAHGYLLHQFLSPIANHRTDEYGGSLENRMRFPLEVFDAVREAFPAERPVWMRVSATDWVPNGWDIEGTIALSHELKARGSAAVHVSTGGVSPQQAIKIGPGYQVPYAQRVKAEVGLPTMAVGLITEAEQAEAIIANNEADIISIARAMLYDPRWPWHAAAKLGASVNAPKQYWRSQPRGLEKLFKDAHFGQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="111" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LE39</accession>
    <name>Q1LE39_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Putative two component transcriptional regulator, winged helix family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Rmet_4725</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="7">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="7">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11587.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519160.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4725"/>
    <dbReference type="DNASU" id="4041586"/>
    <dbReference type="EnsemblBacteria" id="ABF11587">
        <property type="protein sequence ID" value="ABF11587"/>
        <property type="gene ID" value="Rmet_4725"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4725"/>
    <dbReference type="eggNOG" id="COG0745">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_30_0_4"/>
    <dbReference type="OMA" id="ADDYVCR"/>
    <dbReference type="OrthoDB" id="817710at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00383">
        <property type="entry name" value="trans_reg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001867">
        <property type="entry name" value="OmpR/PhoB-type_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016032">
        <property type="entry name" value="Sig_transdc_resp-reg_C-effctor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039420">
        <property type="entry name" value="WalR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48111">
        <property type="entry name" value="PTHR48111"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00486">
        <property type="entry name" value="Trans_reg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00862">
        <property type="entry name" value="Trans_reg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46894">
        <property type="entry name" value="SSF46894"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51755">
        <property type="entry name" value="OMPR_PHOB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="6" id="KW-0614">Plasmid</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Response regulatory" evidence="4">
        <location>
            <begin position="6"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="domain" description="OmpR/PhoB-type" evidence="5">
        <location>
            <begin position="132"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="OmpR/PhoB-type" evidence="3">
        <location>
            <begin position="132"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="2">
        <location>
            <position position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABF11587.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="261" mass="28599" checksum="56F8615F02CB4F1E" modified="2006-05-30" version="1">MNARTNIALLANSSSPLHPLQELLAVAGYQCNVFHAGRDLIQGLGHDTYEMLLIDRELADISAIDVIRTVRAVRNRDVPIVIFASENNDDDLVEALDAGADDYVLRPLAARVLLARIAALRRRMSGTRLRGAMPLRAGPYELSNPGRYATLNGGRIPMTPKEFDLAMLMFANAGRILASRRIEHTIWGHDLPPYSRALAGLVSRMRRTLNLCAENGVTVSVVYAQGYRLDILDQAARHELSAAARAASASSTPARMFKLLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-10-07" version="51" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LE91</accession>
    <name>Q1LE91_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_4673</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11535.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF11535">
        <property type="protein sequence ID" value="ABF11535"/>
        <property type="gene ID" value="Rmet_4673"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4673"/>
    <dbReference type="eggNOG" id="ENOG5033738">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_116688_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193061" evidence="1">
        <location>
            <begin position="22"/>
            <end position="142"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF11535.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="142" mass="16372" checksum="7D02396849639DFB" modified="2006-05-30" version="1">MDRIRLLLLLTFTALHPLAWAAELSESDAGTYEWLKEDRTPMGVLYRFSRSGNKWVAFGKLPGKDWKAVSCDPGCEYRNSTAKEIQTYFPPSFREHNELACIQNMAQVFCRFTPVNEPRSRGYIFIVLVTGKPLPVRTRRLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-10-07" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LEE9</accession>
    <name>Q1LEE9_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Transcriptional regulator, GntR family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Rmet_4615</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="7">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="7">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11477.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4615"/>
    <dbReference type="EnsemblBacteria" id="ABF11477">
        <property type="protein sequence ID" value="ABF11477"/>
        <property type="gene ID" value="Rmet_4615"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4615"/>
    <dbReference type="eggNOG" id="COG1802">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017584_5_1_4"/>
    <dbReference type="OMA" id="DAAGMQH"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07377">
        <property type="entry name" value="WHTH_GntR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.530">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011711">
        <property type="entry name" value="GntR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008920">
        <property type="entry name" value="TF_FadR/GntR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000524">
        <property type="entry name" value="Tscrpt_reg_HTH_GntR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07729">
        <property type="entry name" value="FCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00392">
        <property type="entry name" value="GntR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00035">
        <property type="entry name" value="HTHGNTR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00895">
        <property type="entry name" value="FCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00345">
        <property type="entry name" value="HTH_GNTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48008">
        <property type="entry name" value="SSF48008"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50949">
        <property type="entry name" value="HTH_GNTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0614">Plasmid</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH gntR-type" evidence="5">
        <location>
            <begin position="50"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="20"/>
            <end position="47"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABF11477.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="254" mass="27369" checksum="8447748102FCECC9" modified="2006-05-30" version="1">MISSHVNGWGGSPAGIATTFPLESRPVPHPTFPSHAMSIDSPATAGPASRSASDSVFFGIMNGLELGTFVPGQRLVETDLVEQFGVGRNSVREALQRLAAEGIVDLPRHRGAIIRRLSLQETLDVLDVAERMTGLLARAATRGSSDRTLAQALRASVQALVAAEKAHDGEAFSTARRHFYRTLLDMGDNRELRRLFPTIQMPIVHAQHRLASLRQMRLDDYKRIATAVQAGEPDAAEAAGAAHVQNVRGAILGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LEU3</accession>
    <name>Q1LEU3_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Heavy metal cation tricomponent efflux pump HmuA (CzcA-like)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">czcA2</name>
        <name evidence="4" type="ordered locus">Rmet_4468</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="5">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="5">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the resistance-nodulation-cell division (RND) (TC 2.A.6) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11333.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518935.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4468"/>
    <dbReference type="EnsemblBacteria" id="ABF11333">
        <property type="protein sequence ID" value="ABF11333"/>
        <property type="gene ID" value="Rmet_4468"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4468"/>
    <dbReference type="eggNOG" id="COG3696">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002755_1_2_4"/>
    <dbReference type="OMA" id="QVMMLKG"/>
    <dbReference type="OrthoDB" id="91817at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008324">
        <property type="term" value="F:cation transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2090.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027463">
        <property type="entry name" value="AcrB_DN_DC_subdom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001036">
        <property type="entry name" value="Acrflvin-R"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004763">
        <property type="entry name" value="CzcA/CusA/SilA/NccA/HelA/CnrA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32063">
        <property type="entry name" value="PTHR32063"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00873">
        <property type="entry name" value="ACR_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00702">
        <property type="entry name" value="ACRIFLAVINRP"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82714">
        <property type="entry name" value="SSF82714"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00914">
        <property type="entry name" value="2A0601"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0614">Plasmid</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="346"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="369"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="452"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="484"/>
            <end position="512"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="533"/>
            <end position="556"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="881"/>
            <end position="900"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="907"/>
            <end position="926"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="932"/>
            <end position="957"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="982"/>
            <end position="1004"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="1010"/>
            <end position="1033"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF11333.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="1043" mass="112862" checksum="41DEEF852D3CA4FF" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="96" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LF13</accession>
    <name>Q1LF13_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Putrescine transporter subunit: membrane component of ABC superfamily binding-protein-dependent transport systems inner membrane component</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">potH</name>
        <name evidence="7" type="ordered locus">Rmet_4398</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="8">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="8">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cell membrane</location>
            <topology evidence="4">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the binding-protein-dependent transport system permease family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11263.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518880.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4398"/>
    <dbReference type="EnsemblBacteria" id="ABF11263">
        <property type="protein sequence ID" value="ABF11263"/>
        <property type="gene ID" value="Rmet_4398"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4398"/>
    <dbReference type="eggNOG" id="COG1176">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016047_18_3_4"/>
    <dbReference type="OMA" id="RIYAWMI"/>
    <dbReference type="OrthoDB" id="1488093at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="SSF161098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0614">Plasmid</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2 4" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="12"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="94"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="125"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="178"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="277"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="6">
        <location>
            <begin position="90"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="311"/>
            <end position="338"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABF11263.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="338" mass="36883" checksum="4F9B0E18F845070F" modified="2006-05-30" version="1">MITRADRLARWLVCGIPIAFLLVFFGIPSAIMIVAAFRTPAESGGLLPLIPPDQGLTTAAAITMIRHWPDLFTLDNLRELIQEPLYAGLFLKSAGYAALTTAICLVAAYPLAWWIARSDKRRRDLLLILVILPFWSNFLIRIYAWMIILSPRGYLMHGVNSFIGWMGFAPVEALYTPAAVILVLVYVHLPFFILPLYASLEKQDPLLLDAARDLGANGWHCFWQVIWPLSLPGVYAGIALVFTPCLGIFAVPELVGGTRSILIGNLIKQQFLDSRDWPLGSALSLALVGIVLALIGLATLAARVKGTARAASQRGTHKSAHRPAQPAPAAVATGRRSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="70" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LF28</accession>
    <name>Q1LF28_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Lipid A biosynthesis acyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Rmet_4383</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="8">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="8">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11248.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518865.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4383"/>
    <dbReference type="EnsemblBacteria" id="ABF11248">
        <property type="protein sequence ID" value="ABF11248"/>
        <property type="gene ID" value="Rmet_4383"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4383"/>
    <dbReference type="eggNOG" id="COG4261">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_049421_2_1_4"/>
    <dbReference type="OMA" id="YPLQWFN"/>
    <dbReference type="OrthoDB" id="1106624at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016746">
        <property type="term" value="F:acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009247">
        <property type="term" value="P:glycolipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="CDD" id="cd07984">
        <property type="entry name" value="LPLAT_LABLAT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014548">
        <property type="entry name" value="Ac_Trasf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004960">
        <property type="entry name" value="LipA_acyltrans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30606">
        <property type="entry name" value="PTHR30606"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03279">
        <property type="entry name" value="Lip_A_acyltrans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF028561">
        <property type="entry name" value="Ac_Trasf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 7" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="2" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="1" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0614">Plasmid</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="20"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="45"/>
            <end position="65"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABF11248.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="343" mass="38616" checksum="EE105A2F093108D0" modified="2006-05-30" version="1">MSTLHPETQGAAPRRRTPHWSAIGEATCVWGIWLLYGLYRAFGRLLFRVVMYPVVVYFWLAYPLARRSSMEYLARVEAATGAIGTQPTWRQSLRHLFSFGDTMLDKLLAIGGRYRFEHVSKEGGDVLLRQIASGRGGIIMTAHVGCLELCRAMANRLNGLRLTVLVHSAHAERFNRILARLDPDSMLQLYQIDDISPATALELSARVEAGEFVAIAGDRAPAHGGRAVRVPFLGQPARFPVGPYVLASLLKCPLYAMGCVREGRGHLIRFTELERVVTLPRATREARLQALAATYSRWLEDLVARSPYDWFNFYDFWADDPISIGRRDMQQPAQERDAKNPES</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LF48</accession>
    <name>Q1LF48_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Fumarylacetoacetate (FAA) hydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_4363</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11228.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518845.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4363"/>
    <dbReference type="EnsemblBacteria" id="ABF11228">
        <property type="protein sequence ID" value="ABF11228"/>
        <property type="gene ID" value="Rmet_4363"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4363"/>
    <dbReference type="eggNOG" id="COG0179">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028458_4_2_4"/>
    <dbReference type="OMA" id="ERQNYPL"/>
    <dbReference type="OrthoDB" id="775108at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.850.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018833">
        <property type="entry name" value="DUF2437"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011234">
        <property type="entry name" value="Fumarylacetoacetase-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036663">
        <property type="entry name" value="Fumarylacetoacetase_C_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10370">
        <property type="entry name" value="DUF2437"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01557">
        <property type="entry name" value="FAA_hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56529">
        <property type="entry name" value="SSF56529"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF2437" evidence="2">
        <location>
            <begin position="5"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="domain" description="FAA_hydrolase" evidence="1">
        <location>
            <begin position="58"/>
            <end position="256"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF10370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF11228.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="258" mass="27648" checksum="04CB54EAFA3516D3" modified="2006-05-30" version="1">MQQTWIRFRSPRGDTGFGLVDGDRVIVYDGPGYTGSKPTGAVLPRDEVHLLAPCEPGKIVALWNNFHALARKLEKPVPLHPLFLMKPASSLAGPGDAIERPASYRGKIVYEGELGIVIGRKCRNVGVSEAASHIFGYTVVNDVTAAELITEDPNFPQWTRAKGYDTFGCLGPGIVTGFDWRAASVVTRLDGVERQNYPLADMVFSPEEQVSRISQDLTLMPGDVIACGTSLGVGSMKDGAVVEVSIAGIGALVNHVKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="99" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LFE9</accession>
    <name>Q1LFE9_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Transcriptional regulator, LysR family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_4261</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the LysR transcriptional regulatory family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11127.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4261"/>
    <dbReference type="EnsemblBacteria" id="ABF11127">
        <property type="protein sequence ID" value="ABF11127"/>
        <property type="gene ID" value="Rmet_4261"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4261"/>
    <dbReference type="eggNOG" id="COG0583">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039613_16_2_4"/>
    <dbReference type="OMA" id="GQAYYRH"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005119">
        <property type="entry name" value="LysR_subst-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000847">
        <property type="entry name" value="Tscrpt_reg_HTH_LysR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00126">
        <property type="entry name" value="HTH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03466">
        <property type="entry name" value="LysR_substrate"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50931">
        <property type="entry name" value="HTH_LYSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH lysR-type" evidence="2">
        <location>
            <begin position="4"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF11127.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="303" mass="33708" checksum="F664E3A7504C71AF" modified="2006-05-30" version="1">MPMQDLNDLYYFAQSVEHGGFAPAGRALGMPKSKLSRRIAMLEERLGVRLIQRSTRQFAVTEIGRVYYEHCKAMLMQAEAAQEAIEVIQAEPRGTLRMTCPVALLQVHVGTILSEFMARHPLLTVHLEATNRRVDPVAEAIDLAIRVRPPPLQDSDLVIRVLAERSQCLVASPALAQAHGMPPAPAELSDWPSLAIGTPQQDHTWHLYGPDGVQATIHHRPRLVTGDMIALRNAAVAGVGVCQLPVMMVRDQLTDGSLVRLLPDWAPRREILHAVFPSRRGLLPGVRLLIDYLAERFEKMEED</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="69" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LFV2</accession>
    <name>Q1LFV2_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Acetone carboxylase gamma subunit</fullName>
            <ecNumber evidence="1">6.4.1.6</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">acxC</name>
        <name evidence="1" type="ordered locus">Rmet_4107</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EC" id="6.4.1.6" evidence="1"/>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10974.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518602.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4107"/>
    <dbReference type="EnsemblBacteria" id="ABF10974">
        <property type="protein sequence ID" value="ABF10974"/>
        <property type="gene ID" value="Rmet_4107"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4107"/>
    <dbReference type="eggNOG" id="COG4647">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1641736_0_0_4"/>
    <dbReference type="OMA" id="VIKCSCG"/>
    <dbReference type="OrthoDB" id="1584977at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018710">
        <property type="term" value="F:acetone carboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016750">
        <property type="entry name" value="Aceto_COase_bsu/gsu"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08882">
        <property type="entry name" value="Acetone_carb_G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF019217">
        <property type="entry name" value="Acetone_carboxlyase_gsu"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0436">Ligase</keyword>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF10974.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="169" mass="19799" checksum="EC1662F9B19CC0FA" modified="2006-05-30" version="1">MSVYTNEQVDHLVEGKLDWETTFRMLSMPKDEGRFEQYLAALQAKVSFPDRIVLPLGPHMYIVQSAASKKWVVKCDCGHEFCDYRENWKLHASIYVRDTEEAMAEVYPTLMAPDTSWQVYREYYCPSCGAMHDVEAPTPWYPVIHDFEPDIEAFYKEWVHLPVPERAPD</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LG79</accession>
    <name>Q1LG79_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">ABC-type uncharacterized transport system, permease component</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">livM4</name>
        <name evidence="8" type="ordered locus">Rmet_3979</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="9">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="9">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10847.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518487.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3979"/>
    <dbReference type="EnsemblBacteria" id="ABF10847">
        <property type="protein sequence ID" value="ABF10847"/>
        <property type="gene ID" value="Rmet_3979"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3979"/>
    <dbReference type="eggNOG" id="COG4177">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031365_2_2_4"/>
    <dbReference type="OMA" id="IEMVTMA"/>
    <dbReference type="OrthoDB" id="1456257at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015658">
        <property type="term" value="F:branched-chain amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06581">
        <property type="entry name" value="TM_PBP1_LivM_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001851">
        <property type="entry name" value="ABC_transp_permease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043428">
        <property type="entry name" value="LivM-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30482">
        <property type="entry name" value="PTHR30482"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02653">
        <property type="entry name" value="BPD_transp_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0614">Plasmid</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="7"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="30"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="56"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="82"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="110"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="133"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="161"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="209"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="247"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="283"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="317"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="323"/>
            <end position="342"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ABF10847.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="342" mass="36831" checksum="5E374A109F2358B6" modified="2006-05-30" version="1">MKSMQGKIGWTLLFVAAIVFPLAVPNSYYLTVMTLAFIMAIATLGLNLLTGYTGQLNLAHGGFMAIGAYTLGILTVDHQMPYWLAFALSGVVCMVLGYVVGTVSLRLKGHYFSIFTMCVGYIIYLLIEKWESLTHGAVGLIGIPVPASIGPIAFDSVQAQYYLVLVFLALGTFVMHRIVSSLVGRSFMAVRNSDALAEALGINLMRTKVLSFVLSVGYAGFAGALYAGQVRFLGPDIARTDITFDMVMAMLVGGTGTLLGPLLGSLLVPWVTQTLQFLQDYRMLVFGPVLILLIIFVPDGIVGSFLKRQVRRAAAARRGQMAAPRPTQTTSVPTTRPGAENA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LGD8</accession>
    <name>Q1LGD8_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Extra-cytoplasmic solute receptor protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">bug</name>
        <name evidence="4" type="ordered locus">Rmet_3920</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="5">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="5">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Periplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the UPF0065 (bug) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10788.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518435.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3920"/>
    <dbReference type="EnsemblBacteria" id="ABF10788">
        <property type="protein sequence ID" value="ABF10788"/>
        <property type="gene ID" value="Rmet_3920"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3920"/>
    <dbReference type="eggNOG" id="COG3181">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045683_0_2_4"/>
    <dbReference type="OMA" id="NVHYRGD"/>
    <dbReference type="OrthoDB" id="447734at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042597">
        <property type="term" value="C:periplasmic space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005064">
        <property type="entry name" value="BUG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042100">
        <property type="entry name" value="Bug_dom1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006311">
        <property type="entry name" value="TAT_signal"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42928">
        <property type="entry name" value="PTHR42928"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03401">
        <property type="entry name" value="TctC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017082">
        <property type="entry name" value="YflP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51318">
        <property type="entry name" value="TAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-0675">Receptor</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193516" evidence="3">
        <location>
            <begin position="27"/>
            <end position="325"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF10788.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="325" mass="34130" checksum="A6C639E80A006311" modified="2006-05-30" version="1">MNPSQLTRRRLLGAMALAGLVPPAFGNAYPSKAVRFVSPYGPGGSNDTSARILAEVLSRKYGQQVVVENKPGAGTRVASESVAHAAADGYTLLWAAAPFAINTGAGLSQRFDIHKDFVAVGPRVLGPLFLIVNANSSVKTVADFVKLAKQKPDGVNFASPGIGSGPHLTAELFAWQAGFKVVNVHYRGDATAYTELLAGRVDATLTAITTALPFIQAGKLRVIAVAAEQRTPVYQDAPTFAEQGVPGVVGYGWFGIVAPAGTPAPIVRQLNQDASQALSDAQVRQKLLALGLQPEPGSSADFAAFIDSEVKKWGTLVRTRGLVLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LGE4</accession>
    <name>Q1LGE4_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Putative transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Rmet_3914</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="5">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="5">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10782.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3914"/>
    <dbReference type="EnsemblBacteria" id="ABF10782">
        <property type="protein sequence ID" value="ABF10782"/>
        <property type="gene ID" value="Rmet_3914"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3914"/>
    <dbReference type="eggNOG" id="COG1309">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_105787_0_0_4"/>
    <dbReference type="OMA" id="QIAAACQ"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001647">
        <property type="entry name" value="HTH_TetR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041669">
        <property type="entry name" value="TetR_C_15"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17918">
        <property type="entry name" value="TetR_C_15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00440">
        <property type="entry name" value="TetR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50977">
        <property type="entry name" value="HTH_TETR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0614">Plasmid</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH tetR-type" evidence="3">
        <location>
            <begin position="42"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="H-T-H motif" evidence="1">
        <location>
            <begin position="65"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="9"/>
            <end position="37"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF10782.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="234" mass="26220" checksum="17AE74D9DC8B5F48" modified="2006-05-30" version="1">MRIRIRKSAMQSPITSAPSTSSPFPRVDTTSILQPPRQRRARETEQALLAAGRKLLAERDFAAVSVAQIAAACQVSVGAFYGRFRDKMAFFDALRTLVMEESDASVARYMAEHRWGEVPARQIVEKTMRFLVNGCHNNRGVIRASLKHSSTRPEEWLPHQKNGEEIVERLVALLTPRLPGPAEANEVRVRFAMQMVFGMLVNAVLNNPGPLRLDDERLSGEMTRMVACYLGLES</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="96" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LGF9</accession>
    <name>Q1LGF9_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Acyl-CoA dehydrogenase</fullName>
            <ecNumber evidence="8">1.3.8.4</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Rmet_3899</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="9">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="9">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the acyl-CoA dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.3.8.4" evidence="8"/>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10767.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518414.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3899"/>
    <dbReference type="EnsemblBacteria" id="ABF10767">
        <property type="protein sequence ID" value="ABF10767"/>
        <property type="gene ID" value="Rmet_3899"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3899"/>
    <dbReference type="eggNOG" id="COG1960">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018204_0_2_4"/>
    <dbReference type="OMA" id="MQSMAVT"/>
    <dbReference type="OrthoDB" id="760677at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008470">
        <property type="term" value="F:isovaleryl-CoA dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.540.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.110.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006089">
        <property type="entry name" value="Acyl-CoA_DH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006091">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046373">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036250">
        <property type="entry name" value="AcylCo_DH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009075">
        <property type="entry name" value="AcylCo_DH/oxidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013786">
        <property type="entry name" value="AcylCoA_DH/ox_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037069">
        <property type="entry name" value="AcylCoA_DH/ox_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009100">
        <property type="entry name" value="AcylCoA_DH/oxidase_NM_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00441">
        <property type="entry name" value="Acyl-CoA_dh_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02770">
        <property type="entry name" value="Acyl-CoA_dh_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02771">
        <property type="entry name" value="Acyl-CoA_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47203">
        <property type="entry name" value="SSF47203"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56645">
        <property type="entry name" value="SSF56645"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00073">
        <property type="entry name" value="ACYL_COA_DH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0274">FAD</keyword>
    <keyword evidence="3" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-0614">Plasmid</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Acyl-CoA_dh_N" evidence="7">
        <location>
            <begin position="13"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA_dh_M" evidence="6">
        <location>
            <begin position="123"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA_dh_1" evidence="5">
        <location>
            <begin position="234"/>
            <end position="379"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF02770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF02771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ABF10767.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="389" mass="43241" checksum="214883D27576C66A" modified="2006-05-30" version="1">MHAAQDQDLIYPEIREAVRDLCKQFDSAYWQRIEEHSAFPEEFVQALTHAGWLSALIPEAYGGSGLPLTAASVIMEEINRNGGNSGACHGQMYVMGCLLRHGSEAQKQRWLPGIASGELRMQSMAVTEPTTGTDTTKLKTTAVRRGDKYVINGQKVWISRVQHSDLMLLLARTTPLDQVKKKSEGLSVFVVDLREAIGNGLTVQPIRNMVNHETNELFFDNLEIPAENLIGEEGKGLKYILDGLNAERILIAAECIGDGYWFVERASSYARERIVFDRPIGQNQGVQFPIARSFVNVEAASLMRYKAAQLFDAGKPCGKEANMAKLLAADASWEAANVCLQTHGGFGFAAEYDIERKFRETRLYQVAPISTNLILSFVAEHVLELPRSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="60" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LGV1</accession>
    <name>Q1LGV1_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_3754</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10625.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008641189.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3754"/>
    <dbReference type="EnsemblBacteria" id="ABF10625">
        <property type="protein sequence ID" value="ABF10625"/>
        <property type="gene ID" value="Rmet_3754"/>
    </dbReference>
    <dbReference type="GeneID" id="60826308"/>
    <dbReference type="KEGG" id="rme:Rmet_3754"/>
    <dbReference type="eggNOG" id="ENOG503347X">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_185880_0_0_4"/>
    <dbReference type="OMA" id="EANEEWN"/>
    <dbReference type="OrthoDB" id="2043857at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF10625.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="105" mass="11972" checksum="C97B64FF2E72EAA1" modified="2006-05-30" version="1">MIHHNTDPTFRGLPLTAEQDSEIRHYLNRKSRLGKPWNTPELRGMLEDMLLPPSTEDDSQTDIPRDALAAAERAAAFVDQDMEPVEAYEELHAAMEAESMKRDTH</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LH34</accession>
    <name>Q1LH34_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Short-chain dehydrogenase/reductase SDR</fullName>
            <ecNumber evidence="1">1.1.1.100</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_3670</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EC" id="1.1.1.100" evidence="1"/>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10542.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008645350.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3670"/>
    <dbReference type="EnsemblBacteria" id="ABF10542">
        <property type="protein sequence ID" value="ABF10542"/>
        <property type="gene ID" value="Rmet_3670"/>
    </dbReference>
    <dbReference type="GeneID" id="60826225"/>
    <dbReference type="KEGG" id="rme:Rmet_3670"/>
    <dbReference type="eggNOG" id="COG1028">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010194_1_2_4"/>
    <dbReference type="OMA" id="DEMMEVN"/>
    <dbReference type="OrthoDB" id="1601931at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004316">
        <property type="term" value="F:3-oxoacyl-[acyl-carrier-protein] reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00061">
        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF10542.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="243" mass="25202" checksum="EBD02A9C446B4559" modified="2006-05-30" version="1">MSLPVATLVTGASSGIGRAIAEMLLADGVTRVINVDYAAPTWSHPNMTFFQADLTNADATRAVAEQVTSQFAVTRLVNNAGATRPGTADTATAEDLDYVVGLTLQATMLLTQACLPAMRAAGFGRIVNMASRAALGKPERVVYSAAKAGLVGMTRTLAMELGGDGITVNAVAPGPIATELFRRSNPEGSEQTRRILASIAVKRMGTPEDVARASLFFLAPENGFVTGQVLYVCGGTTLGVAPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="62" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LH79</accession>
    <name>Q1LH79_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF58 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Rmet_3625</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="5">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="5">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10497.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3625"/>
    <dbReference type="EnsemblBacteria" id="ABF10497">
        <property type="protein sequence ID" value="ABF10497"/>
        <property type="gene ID" value="Rmet_3625"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3625"/>
    <dbReference type="eggNOG" id="COG1721">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_054568_0_1_4"/>
    <dbReference type="OMA" id="TAETRFP"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002881">
        <property type="entry name" value="DUF58"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01882">
        <property type="entry name" value="DUF58"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0614">Plasmid</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="21"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="47"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="domain" description="DUF58" evidence="3">
        <location>
            <begin position="211"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="183"/>
            <end position="203"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF10497.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="316" mass="34492" checksum="B6258A360DFC8525" modified="2006-05-30" version="1">MWLRRKRAPVNGVVTLDRRHLYILPTRGGIGFSVLLATMLLTSLNYAISLGFALTFLLAGIGMGCMWMAYRNLLDLRVSAGPVTPAFVGECAIFTLHVDNQDAAARIGIEARTPLSGADTETAALTLDGVSSGTLPVRLSAIRRGRLVLPRVTLSSRFPFGLFRVWSYADLGLATVVYPAPEASPPPLPRHPQSDDADDGAASFAADDGIDQLRRYRPGDPLHRIAWKHSARTGKWLTRSGHTPQQPSCWIDWNTLPPAMDTEARLSRLCAWVLMAGEVSEIGLRLPGVEITPGRGSAHVRACLEALALWPERRAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LHL2</accession>
    <name>Q1LHL2_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Esterase/lipase</fullName>
            <ecNumber evidence="5">3.1.1.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Rmet_3492</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the 'GDXG' lipolytic enzyme family.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.-" evidence="5"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10364.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517920.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3492"/>
    <dbReference type="ESTHER" id="ralme-q1lhl2">
        <property type="family name" value="Hormone-sensitive_lipase_like"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF10364">
        <property type="protein sequence ID" value="ABF10364"/>
        <property type="gene ID" value="Rmet_3492"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3492"/>
    <dbReference type="eggNOG" id="COG0657">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012494_6_4_4"/>
    <dbReference type="OMA" id="LWYPSTM"/>
    <dbReference type="OrthoDB" id="1361074at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013094">
        <property type="entry name" value="AB_hydrolase_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002168">
        <property type="entry name" value="Lipase_GDXG_HIS_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033140">
        <property type="entry name" value="Lipase_GDXG_put_SER_AS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07859">
        <property type="entry name" value="Abhydrolase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="SSF53474"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01173">
        <property type="entry name" value="LIPASE_GDXG_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01174">
        <property type="entry name" value="LIPASE_GDXG_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Abhydrolase_3" evidence="4">
        <location>
            <begin position="79"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="active site" evidence="3">
        <location>
            <position position="157"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF07859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABF10364.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="318" mass="34535" checksum="569933EA6171ED5F" modified="2006-05-30" version="1">MAIDPQLLSYYRQMADLYANEPIPEDAAARRSRFEDVAARSRQPDTEEISVTDLAIPVDGAALTVRLFRPANVEQPRLIVYFHGGGWVVGSPETHHTVAALLAADTQCVVASVDYRLAPEYAFPTPCDDAVAAVQWLAASRESLGVHPDFLAVAGDSAGAHLAAAAARVVNEQRRAHIVQAQLLIYPVAAPLLTTESYRAFANGPGLTRDEMAWYWTQFIGAEELAKGAEQDDPRIHLMASPPAMLPPASVVLVAANDVLRDDGLAYANFLVRHDAPVITIEASGMTHGFARLQPDAPRAREWMRRAAEAFTGVFDDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LHL7</accession>
    <name>Q1LHL7_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_3487</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10359.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517916.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3487"/>
    <dbReference type="EnsemblBacteria" id="ABF10359">
        <property type="protein sequence ID" value="ABF10359"/>
        <property type="gene ID" value="Rmet_3487"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3487"/>
    <dbReference type="eggNOG" id="COG0457">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_419122_0_0_4"/>
    <dbReference type="OMA" id="HWIDLLD"/>
    <dbReference type="OrthoDB" id="193067at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_repeat"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="SSF48452"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="492"/>
            <end position="519"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF10359.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="651" mass="71013" checksum="4E6EA52D115F4D02" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LHN1</accession>
    <name>Q1LHN1_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Cytochrome c family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">Rmet_3473</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10345.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517903.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3473"/>
    <dbReference type="EnsemblBacteria" id="ABF10345">
        <property type="protein sequence ID" value="ABF10345"/>
        <property type="gene ID" value="Rmet_3473"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3473"/>
    <dbReference type="eggNOG" id="COG3245">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_082349_1_0_4"/>
    <dbReference type="OMA" id="VNDRIKP"/>
    <dbReference type="OrthoDB" id="1469969at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.760.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009056">
        <property type="entry name" value="Cyt_c-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036909">
        <property type="entry name" value="Cyt_c-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002323">
        <property type="entry name" value="Cyt_CIE"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40942">
        <property type="entry name" value="PTHR40942"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13442">
        <property type="entry name" value="Cytochrome_CBB3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00607">
        <property type="entry name" value="CYTCHROMECIE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46626">
        <property type="entry name" value="SSF46626"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51007">
        <property type="entry name" value="CYTC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0249">Electron transport</keyword>
    <keyword evidence="2 6" id="KW-0349">Heme</keyword>
    <keyword evidence="5 6" id="KW-0408">Iron</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="21"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="domain" description="Cytochrome c" evidence="8">
        <location>
            <begin position="79"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="domain" description="Cytochrome c" evidence="8">
        <location>
            <begin position="214"/>
            <end position="293"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ABF10345.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="294" mass="29327" checksum="823F1891B4B7DECC" modified="2006-05-30" version="1">MSDVHQENDHHESPIKTPKQLIVVVIAAFLIPILVIILLVNFVGHGTREGAGSTQSAEAINDRIKPVASLEIKDPNAPRVLKTGEEVYKAVCAACHASGAAGAPKYGVAGDWAGRIGQGFDGLMKSVLNGKNAMPARAGTTPDDLSDYELARAVVYMADAGGAKFPEPAAPAAGAPAQAAAPAADAPAAAPAAAAPAAAAAPAPAAAAAAPAAASAETGKKVYEQVCAACHASGVAGAPKFGDKAAWAPRLKEGMDAVHNYALKGKGVMPPKGGYAGPDADVIAASNYMAEAGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="59" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LHQ0</accession>
    <name>Q1LHQ0_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_3454</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10326.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008646158.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF10326">
        <property type="protein sequence ID" value="ABF10326"/>
        <property type="gene ID" value="Rmet_3454"/>
    </dbReference>
    <dbReference type="GeneID" id="60822559"/>
    <dbReference type="KEGG" id="rme:Rmet_3454"/>
    <dbReference type="HOGENOM" id="CLU_1863545_0_0_4"/>
    <dbReference type="OMA" id="GAKQGKY"/>
    <dbReference type="OrthoDB" id="1730664at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193508" evidence="1">
        <location>
            <begin position="21"/>
            <end position="115"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF10326.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="115" mass="12039" checksum="110C0CF684F04822" modified="2006-05-30" version="1">MNAKRILGGALVLAAFAASAAIAAPAATKKAGKFDVYADAARVGKFDVYSDGAKIGKYDFYTDGQRASKFDPYSEGTRQGKFDSFSEGAHAFSGEISASSLDNSRNGGELYGYSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="98" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LHW1</accession>
    <name>Q1LHW1_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Type II secretion system protein E</fullName>
            <shortName evidence="3">T2SS protein E</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">Type II traffic warden ATPase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5" type="primary">gspE</name>
        <name evidence="5" type="ordered locus">Rmet_3393</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">ATPase component of the type II secretion system required for the energy-dependent secretion of extracellular factors such as proteases and toxins from the periplasm. Acts as a molecular motor to provide the energy that is required for assembly of the pseudopilus and the extrusion of substrates generated in the cytoplasm.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell inner membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the GSP E family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10265.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3393"/>
    <dbReference type="EnsemblBacteria" id="ABF10265">
        <property type="protein sequence ID" value="ABF10265"/>
        <property type="gene ID" value="Rmet_3393"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3393"/>
    <dbReference type="eggNOG" id="COG2804">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013446_2_0_4"/>
    <dbReference type="OMA" id="RSRIDGM"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
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        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="term" value="C:type II protein secretion system complex"/>
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        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
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        <property type="term" value="F:ATP binding"/>
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        <property type="project" value="UniProtKB-UniRule"/>
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        <property type="term" value="P:protein secretion by the type II secretion system"/>
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        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001482">
        <property type="entry name" value="T2SS/T4SS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037257">
        <property type="entry name" value="T2SS_E_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013369">
        <property type="entry name" value="T2SS_GspE"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00437">
        <property type="entry name" value="T2SSE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160246">
        <property type="entry name" value="SSF160246"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02533">
        <property type="entry name" value="type_II_gspE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00662">
        <property type="entry name" value="T2SP_E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-0653">Protein transport</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="T2SP_E" evidence="4">
        <location>
            <begin position="331"/>
            <end position="345"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006611"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU366070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS00662"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABF10265.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="513" mass="55980" checksum="E8221014464620C0" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-12-02" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LHW4</accession>
    <name>Q1LHW4_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="12">General secretion pathway protein M</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">gspM</name>
        <name evidence="12" type="ordered locus">Rmet_3390</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell inner membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the GSP M family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10262.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3390"/>
    <dbReference type="EnsemblBacteria" id="ABF10262">
        <property type="protein sequence ID" value="ABF10262"/>
        <property type="gene ID" value="Rmet_3390"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3390"/>
    <dbReference type="eggNOG" id="COG3149">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_118900_1_0_4"/>
    <dbReference type="OMA" id="WMQREPR"/>
    <dbReference type="OrthoDB" id="1991021at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015627">
        <property type="term" value="C:type II protein secretion system complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015628">
        <property type="term" value="P:protein secretion by the type II secretion system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007690">
        <property type="entry name" value="T2SS_GspM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023229">
        <property type="entry name" value="T2SS_M_periplasmic_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04612">
        <property type="entry name" value="T2SSM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103054">
        <property type="entry name" value="SSF103054"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0653">Protein transport</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="41"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004377"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010637"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ABF10262.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="188" mass="20581" checksum="ED8C883F220CF499" modified="2006-05-30" version="1">MRSGMSTNMATRLARFKPRVPDRWREQVDVFWTARNPREQAILGGGAVVLVLVFGYLLLWEPASVEGARLMRNLPVLRADLAEMETLAQEARGLSANRAPSLRGDALTQALQDSLVQHGLKATRLMTTGDNAVQIQLDKVPFGSVAGWLQDVRLQQRLKVTDTRISYVGATALVNMTATLQGPGTGGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LHW6</accession>
    <name>Q1LHW6_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Type II secretion system protein K</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">gspK</name>
        <name evidence="12" type="ordered locus">Rmet_3388</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 11">Cell inner membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the GSP K family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10260.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3388"/>
    <dbReference type="EnsemblBacteria" id="ABF10260">
        <property type="protein sequence ID" value="ABF10260"/>
        <property type="gene ID" value="Rmet_3388"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3388"/>
    <dbReference type="eggNOG" id="COG3156">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_057294_1_1_4"/>
    <dbReference type="OMA" id="THYFLVY"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009306">
        <property type="term" value="P:protein secretion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005628">
        <property type="entry name" value="GspK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038072">
        <property type="entry name" value="GspK_central_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045584">
        <property type="entry name" value="Pilin-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38831">
        <property type="entry name" value="PTHR38831"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03934">
        <property type="entry name" value="T2SSK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002786">
        <property type="entry name" value="XcpX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF158544">
        <property type="entry name" value="SSF158544"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54523">
        <property type="entry name" value="SSF54523"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 11" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="5 11" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0653">Protein transport</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4 11" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007246"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRNR" id="PIRNR002786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ABF10260.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="336" mass="37032" checksum="2D4754FA6175975E" modified="2006-05-30" version="1">MRHHPPPSFMRSRQRGAAVVTALLIVTLAVVVVSGMLWRQQVQIRSIENQRLMAQAQWIERAAVDWARLILRDDQRRSNVDYLGEPWSVPVAETRLSDFLGAGLRTDQAGETSFLSGRILDAQARFNLTNLYQSTSGESGLTISIDPASMQAFGRLLQTLGMNAALAESTAQYVLRAAKGGSGDGQPVPRPPDSVQSLLAIPGFTPEMIDTLEPFVTVLPERTTVNANTADAEVLAAVIDKLPLDRARELVRQRDRAYFNNTGDITTQLQTIAPKAVITANTLDVSSRYFLIYGLVRHDRARRLKVSLVERQNVQGSGNTTRVVWSRDADAMPPSN</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-12-12" modified="2022-05-25" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LI64</accession>
    <name>RL17_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">50S ribosomal protein L17</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rplQ</name>
        <name type="ordered locus">Rmet_3290</name>
    </gene>
    <organism>
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="1">Part of the 50S ribosomal subunit. Contacts protein L32.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the bacterial ribosomal protein bL17 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10162.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008642987.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q1LI64"/>
    <dbReference type="SMR" id="Q1LI64"/>
    <dbReference type="STRING" id="266264.Rmet_3290"/>
    <dbReference type="EnsemblBacteria" id="ABF10162">
        <property type="protein sequence ID" value="ABF10162"/>
        <property type="gene ID" value="Rmet_3290"/>
    </dbReference>
    <dbReference type="GeneID" id="60826627"/>
    <dbReference type="KEGG" id="rme:Rmet_3290"/>
    <dbReference type="eggNOG" id="COG0203">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_074407_2_0_4"/>
    <dbReference type="OMA" id="YAVTELF"/>
    <dbReference type="OrthoDB" id="1838961at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1030.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01368">
        <property type="entry name" value="Ribosomal_L17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000456">
        <property type="entry name" value="Ribosomal_L17"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036373">
        <property type="entry name" value="Ribosomal_L17_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14413">
        <property type="entry name" value="PTHR14413"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01196">
        <property type="entry name" value="Ribosomal_L17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64263">
        <property type="entry name" value="SSF64263"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00059">
        <property type="entry name" value="L17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01167">
        <property type="entry name" value="RIBOSOMAL_L17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <feature type="chain" id="PRO_0000267921" description="50S ribosomal protein L17">
        <location>
            <begin position="1"/>
            <end position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01368"/>
        </source>
    </evidence>
    <sequence length="131" mass="15064" checksum="A487C920F1EE153C" modified="2006-05-30" version="1">MRHRHGLRKLNRTSSHRLAMLRNMSNSLFQHELIKTTLPKAKELRKVVEPLITLAKKDTVANRRLAFARLRDRDMVTKLFTELGPRYATRPGGYTRILKFGFRQGDNAPMALVELVDRPEITEAPAEEAAE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-02-05" modified="2022-05-25" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LIA5</accession>
    <name>HIS1_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">ATP phosphoribosyltransferase</fullName>
            <shortName evidence="1">ATP-PRT</shortName>
            <shortName evidence="1">ATP-PRTase</shortName>
            <ecNumber evidence="1">2.4.2.17</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">hisG</name>
        <name type="ordered locus">Rmet_3249</name>
    </gene>
    <organism>
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the condensation of ATP and 5-phosphoribose 1-diphosphate to form N'-(5'-phosphoribosyl)-ATP (PR-ATP). Has a crucial role in the pathway because the rate of histidine biosynthesis seems to be controlled primarily by regulation of HisG enzymatic activity.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>1-(5-phospho-beta-D-ribosyl)-ATP + diphosphate = 5-phospho-alpha-D-ribose 1-diphosphate + ATP</text>
            <dbReference type="Rhea" id="RHEA:18473"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <dbReference type="ChEBI" id="CHEBI:73183"/>
            <dbReference type="EC" id="2.4.2.17"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-histidine biosynthesis; L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 1/9.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Heteromultimer composed of HisG and HisZ subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text>Lacks the C-terminal regulatory region which is replaced by HisZ.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the ATP phosphoribosyltransferase family. Short subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.17" evidence="1"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10121.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008643045.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q1LIA5"/>
    <dbReference type="SMR" id="Q1LIA5"/>
    <dbReference type="STRING" id="266264.Rmet_3249"/>
    <dbReference type="EnsemblBacteria" id="ABF10121">
        <property type="protein sequence ID" value="ABF10121"/>
        <property type="gene ID" value="Rmet_3249"/>
    </dbReference>
    <dbReference type="GeneID" id="60825573"/>
    <dbReference type="KEGG" id="rme:Rmet_3249"/>
    <dbReference type="eggNOG" id="COG0040">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038115_2_0_4"/>
    <dbReference type="OMA" id="YVMMDYD"/>
    <dbReference type="OrthoDB" id="1419568at2"/>
    <dbReference type="UniPathway" id="UPA00031">
        <property type="reaction ID" value="UER00006"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003879">
        <property type="term" value="F:ATP phosphoribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000105">
        <property type="term" value="P:histidine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd13595">
        <property type="entry name" value="PBP2_HisGs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01018">
        <property type="entry name" value="HisG_Short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013820">
        <property type="entry name" value="ATP_PRibTrfase_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018198">
        <property type="entry name" value="ATP_PRibTrfase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001348">
        <property type="entry name" value="ATP_PRibTrfase_HisG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024893">
        <property type="entry name" value="ATP_PRibTrfase_HisG_short"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21403">
        <property type="entry name" value="PTHR21403"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01634">
        <property type="entry name" value="HisG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00070">
        <property type="entry name" value="hisG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01316">
        <property type="entry name" value="ATP_P_PHORIBOSYLTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0328">Glycosyltransferase</keyword>
    <keyword id="KW-0368">Histidine biosynthesis</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_1000063303" description="ATP phosphoribosyltransferase">
        <location>
            <begin position="1"/>
            <end position="224"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01018"/>
        </source>
    </evidence>
    <sequence length="224" mass="23953" checksum="30BCF859FD8D5545" modified="2006-05-30" version="1">MSPAFNASPDQLTLALSKGRIFKETLPLLAAAGIQVTEDPETSRKLILPTSDPAVRVIIVRASDVPTYVQYGAADFGVAGKDVLMEHGMAGLYAPIDLNIARCRMSVAVPAGFDYVNAVRQGARLAVATKYVQTAREHFAKKGVHVDLIKLYGSMELGPLVGLSDAIVDLVSTGSTLRANNLVEVEEIVQISSRLVVNQAALKLKRERLAPILDAFERASAALA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LIN8</accession>
    <name>Q1LIN8_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">AAA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_3116</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09988.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008650326.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3116"/>
    <dbReference type="EnsemblBacteria" id="ABF09988">
        <property type="protein sequence ID" value="ABF09988"/>
        <property type="gene ID" value="Rmet_3116"/>
    </dbReference>
    <dbReference type="GeneID" id="60820508"/>
    <dbReference type="KEGG" id="rme:Rmet_3116"/>
    <dbReference type="eggNOG" id="COG2607">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039512_0_0_4"/>
    <dbReference type="OMA" id="NGRCAYQ"/>
    <dbReference type="OrthoDB" id="1657201at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008533">
        <property type="entry name" value="DUF815"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05673">
        <property type="entry name" value="DUF815"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA" evidence="1">
        <location>
            <begin position="85"/>
            <end position="213"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF09988.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="303" mass="33754" checksum="14CDD4F5BCF61435" modified="2006-05-30" version="1">MSDLAARLDGFLARLEQWLPPELTDADWKEAVAFRWRKRQSLFGNIGYLQAIRQLPPIHLGDLKNIDRQKDAIVANTRQFVNRLPANNVLLTGARGTGKSSLIKACLNAFVNDGLRLVEVDKDDLGDLGDIVEQLAQRPERFAIFCDDLSFEDGESGYKSLKSALDGSVAAQSDNVLIYATSNRRHLLPEYMKDNETYQHTSDGEIHPGEVVEEKISLSERFGLWLSFYPPKQDEYLAIVGHWLGHFGCTPADIEAARGDALVWALERGSRSGRVAWQFARDWGGKHGKPFVADPATDSGSKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LIW4</accession>
    <name>Q1LIW4_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Rmet_3040</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09912.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517546.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3040"/>
    <dbReference type="EnsemblBacteria" id="ABF09912">
        <property type="protein sequence ID" value="ABF09912"/>
        <property type="gene ID" value="Rmet_3040"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3040"/>
    <dbReference type="eggNOG" id="COG0476">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG1310">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021897_0_0_4"/>
    <dbReference type="OMA" id="WEPVRRD"/>
    <dbReference type="OrthoDB" id="555145at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008237">
        <property type="term" value="F:metallopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008641">
        <property type="term" value="F:ubiquitin-like modifier activating enzyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028090">
        <property type="entry name" value="JAB_dom_prok"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032865">
        <property type="entry name" value="Prok-E2_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000594">
        <property type="entry name" value="ThiF_NAD_FAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035985">
        <property type="entry name" value="Ubiquitin-activating_enz"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14457">
        <property type="entry name" value="Prok-E2_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14464">
        <property type="entry name" value="Prok-JAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00899">
        <property type="entry name" value="ThiF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69572">
        <property type="entry name" value="SSF69572"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="1" id="KW-0645">Protease</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="ThiF" evidence="6">
        <location>
            <begin position="363"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="domain" description="Prok-JAB" evidence="7">
        <location>
            <begin position="626"/>
            <end position="734"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF14464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ABF09912.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="751" mass="79961" checksum="5E31C9226BEAB4AE" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-09-02" modified="2022-08-03" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LJ45</accession>
    <name>MNMA_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">tRNA-specific 2-thiouridylase MnmA</fullName>
            <ecNumber evidence="1">2.8.1.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">mnmA</name>
        <name type="ordered locus">Rmet_2958</name>
    </gene>
    <organism>
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the 2-thiolation of uridine at the wobble position (U34) of tRNA, leading to the formation of s(2)U34.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>AH2 + ATP + S-sulfanyl-L-cysteinyl-[protein] + uridine(34) in tRNA = 2-thiouridine(34) in tRNA + A + AMP + diphosphate + H(+) + L-cysteinyl-[protein]</text>
            <dbReference type="Rhea" id="RHEA:47032"/>
            <dbReference type="Rhea" id="RHEA-COMP:10131"/>
            <dbReference type="Rhea" id="RHEA-COMP:11726"/>
            <dbReference type="Rhea" id="RHEA-COMP:11727"/>
            <dbReference type="Rhea" id="RHEA-COMP:11728"/>
            <dbReference type="ChEBI" id="CHEBI:13193"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17499"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61963"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="ChEBI" id="CHEBI:87170"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="2.8.1.13"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the MnmA/TRMU family.</text>
    </comment>
    <dbReference type="EC" id="2.8.1.13" evidence="1"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09831.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517496.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q1LJ45"/>
    <dbReference type="SMR" id="Q1LJ45"/>
    <dbReference type="STRING" id="266264.Rmet_2958"/>
    <dbReference type="EnsemblBacteria" id="ABF09831">
        <property type="protein sequence ID" value="ABF09831"/>
        <property type="gene ID" value="Rmet_2958"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2958"/>
    <dbReference type="eggNOG" id="COG0482">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035188_1_0_4"/>
    <dbReference type="OMA" id="AVCTGHY"/>
    <dbReference type="OrthoDB" id="1054741at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016783">
        <property type="term" value="F:sulfurtransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006400">
        <property type="term" value="P:tRNA modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01998">
        <property type="entry name" value="tRNA_Me_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.280">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00144">
        <property type="entry name" value="tRNA_thiouridyl_MnmA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023382">
        <property type="entry name" value="Adenine_a_hdrlase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004506">
        <property type="entry name" value="tRNA-specific_2-thiouridylase"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00420">
        <property type="entry name" value="trmU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-1015">Disulfide bond</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <keyword id="KW-0819">tRNA processing</keyword>
    <keyword id="KW-0820">tRNA-binding</keyword>
    <feature type="chain" id="PRO_0000349765" description="tRNA-specific 2-thiouridylase MnmA">
        <location>
            <begin position="1"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="region of interest" description="Interaction with target base in tRNA" evidence="1">
        <location>
            <begin position="95"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="region of interest" description="Interaction with tRNA" evidence="1">
        <location>
            <begin position="147"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="region of interest" description="Interaction with tRNA" evidence="1">
        <location>
            <begin position="309"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="1">
        <location>
            <position position="100"/>
        </location>
    </feature>
    <feature type="active site" description="Cysteine persulfide intermediate" evidence="1">
        <location>
            <position position="197"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="9"/>
            <end position="16"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="35"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="124"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="site" description="Interaction with tRNA" evidence="1">
        <location>
            <position position="125"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with tRNA" evidence="1">
        <location>
            <position position="342"/>
        </location>
    </feature>
    <feature type="disulfide bond" description="Alternate" evidence="1">
        <location>
            <begin position="100"/>
            <end position="197"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00144"/>
        </source>
    </evidence>
    <sequence length="359" mass="39184" checksum="5A64F408D443C8F5" modified="2006-05-30" version="1">MSGKRVVVGLSGGVDSSVTAWLLKQQGYEVIGLFMKNWEDDDDSEYCSTRQDWLDVASVADVIGVDVEAVNFAAEYKDRVFADFLREYSAGRTPNPDVLCNAEIKFKAFLDHAMSLGANTIATGHYARVRQAANGKFELLKAFDHTKDQSYFLHRLNQAQLSRTLFPLGEIPKTRVREIAAEIGLPNAKKKDSTGICFIGERPFRDFLNRYLPTQPGPMKTPEGKVVGEHIGLAFYTLGQRKGIGLGGSRDGSGDAWYVARKDMASNTLYVVQGHDHPWLLTSELNAADLSWVAGEPPAAGATMAAKTRYRQSDAPCRVVAAEGDALKLSFAEPQWAVTPGQSAVLYDGDVCLGGGIIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LJ68</accession>
    <name>Q1LJ68_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">PilU, Type IV pilus twitching motility protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">pilU</name>
        <name evidence="3" type="ordered locus">Rmet_2935</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the GSP E family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09808.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2935"/>
    <dbReference type="EnsemblBacteria" id="ABF09808">
        <property type="protein sequence ID" value="ABF09808"/>
        <property type="gene ID" value="Rmet_2935"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2935"/>
    <dbReference type="eggNOG" id="COG5008">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013446_4_0_4"/>
    <dbReference type="OMA" id="QGLCRTT"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01131">
        <property type="entry name" value="PilT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006321">
        <property type="entry name" value="PilT/PilU"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001482">
        <property type="entry name" value="T2SS/T4SS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00437">
        <property type="entry name" value="T2SSE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01420">
        <property type="entry name" value="pilT_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00662">
        <property type="entry name" value="T2SP_E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="T2SP_E" evidence="2">
        <location>
            <begin position="203"/>
            <end position="217"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006611"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS00662"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF09808.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="381" mass="42792" checksum="7727D9EE575E6BA3" modified="2006-05-30" version="1">MTMLDRESAAKYINEMLGLMVKNRGSDLFITAEFPPAVKVDGKITPVSQQPLNPTHALTLVCSIMNERQTKEFEDTRECNFAIAVPDVGRFRVSAFIQQGRAGMVLRTINTRIPSVEDLDLPPTLHEIVMSKRGLVIVTGATGSGKSTSLAAMLDHRNAHSYGHIITIEDPIEYVHAHQNCIVTQREVGIDTESWHVALKNTLRQAPDVILIGEIRDRDTMEYAMQYAETGHLCLATLHANNANQAIDRIVNFFPEEKRQQLLIDLSLNLKAMISQRLLPRAGRKGRVPAVEIMIGTPLVADLIFKGEIHELKEVIKKSREQGMISFDQALFELYETEKITYEDALKNADSLNDLRLMIKLHSKRHRDNDLGAGTEHLNVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="54" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LJA6</accession>
    <name>Q1LJA6_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_2897</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09770.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008644951.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2897"/>
    <dbReference type="EnsemblBacteria" id="ABF09770">
        <property type="protein sequence ID" value="ABF09770"/>
        <property type="gene ID" value="Rmet_2897"/>
    </dbReference>
    <dbReference type="GeneID" id="60820667"/>
    <dbReference type="KEGG" id="rme:Rmet_2897"/>
    <dbReference type="eggNOG" id="ENOG5033FTF">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_175384_1_0_4"/>
    <dbReference type="OMA" id="KAKKHHH"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193513" evidence="2">
        <location>
            <begin position="20"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="25"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF09770.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="66" mass="6541" checksum="59EE16599E8B6325" modified="2006-05-30" version="1">MKKLIAALVVGLFATGAFAQASAPAAADAAAPAAKEAKPAKSTSKKHHSSKKHSSKKTEASAPAAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="69" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LJJ3</accession>
    <name>Q1LJJ3_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Sirohydrochlorin cobaltochelatase cobalamin biosynthesis CbiX protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">cbiX</name>
        <name evidence="3" type="ordered locus">Rmet_2810</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09683.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517379.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2810"/>
    <dbReference type="EnsemblBacteria" id="ABF09683">
        <property type="protein sequence ID" value="ABF09683"/>
        <property type="gene ID" value="Rmet_2810"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2810"/>
    <dbReference type="eggNOG" id="COG2138">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_065901_2_2_4"/>
    <dbReference type="OMA" id="WREPFER"/>
    <dbReference type="OrthoDB" id="1573073at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002762">
        <property type="entry name" value="CbiX-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01903">
        <property type="entry name" value="CbiX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0456">Lyase</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF09683.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="134" mass="14205" checksum="AD291C5076E03903" modified="2006-05-30" version="1">MGAGRQGLILFGHGARDARWRDPFDRLHEKLVALLPDCAVRLAFLELMTPSLEDAIDELAAAGVGAITVVPVFFGQGGHLRRDFPLLIDACRAKYPGVEIRASDAVGEVDSVLDAIAAYCALTLSPAPAPDSGH</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LJN5</accession>
    <name>Q1LJN5_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Outer membrane protein, OmpA/MotB family(Modular protein)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">ompA</name>
        <name evidence="6" type="ordered locus">Rmet_2768</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09641.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2768"/>
    <dbReference type="EnsemblBacteria" id="ABF09641">
        <property type="protein sequence ID" value="ABF09641"/>
        <property type="gene ID" value="Rmet_2768"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2768"/>
    <dbReference type="eggNOG" id="COG2885">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016890_6_4_4"/>
    <dbReference type="OMA" id="IGNYMDK"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009279">
        <property type="term" value="C:cell outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07185">
        <property type="entry name" value="OmpA_C-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027367">
        <property type="entry name" value="Gly-zipper_YMGG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006664">
        <property type="entry name" value="OMP_bac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006665">
        <property type="entry name" value="OmpA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006690">
        <property type="entry name" value="OMPA-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036737">
        <property type="entry name" value="OmpA-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13441">
        <property type="entry name" value="Gly-zipper_YMGG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00691">
        <property type="entry name" value="OmpA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01021">
        <property type="entry name" value="OMPADOMAIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103088">
        <property type="entry name" value="SSF103088"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01068">
        <property type="entry name" value="OMPA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51123">
        <property type="entry name" value="OMPA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0998">Cell outer membrane</keyword>
    <keyword evidence="1 3" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="OmpA-like" evidence="5">
        <location>
            <begin position="176"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="258"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="261"/>
            <end position="280"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABF09641.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="294" mass="30587" checksum="6BEB74B70E716F28" modified="2006-05-30" version="1">MPSAELQTITSLTVSGLCTRRHMPIVVRQSWDPACRQAVCGIVLSVFDCIMKVVLPVGGITRQALTCRMALAKHLRESPEGESMNFKLVTVSLVAAAALAGCATEQQTNTAVGTGVGAAVGAGIGNLIGGNTTGTLVGAAVGGALGGATGYNWNAIRGKLNKDTAGTGTQITEQPDGSLKVNIPSQVTFDTDSSTIKPSFRNVLDQVAQTLAQHQDVSANVVGHTDSTGNPQYNMQLSQRRAQSVAGYLGDRGVARTRLSAEGRGQTQPVADNSTEAGRAQNRRVEIFLKPISG</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="99" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LK26</accession>
    <name>Q1LK26_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">FAD-dependent pyridine nucleotide-disulphide oxidoreductase</fullName>
            <ecNumber evidence="5">1.16.1.-</ecNumber>
            <ecNumber evidence="5">1.6.99.3</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">ndh</name>
        <name evidence="5" type="ordered locus">Rmet_2623</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the NADH dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.16.1.-" evidence="5"/>
    <dbReference type="EC" id="1.6.99.3" evidence="5"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09500.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2623"/>
    <dbReference type="EnsemblBacteria" id="ABF09500">
        <property type="protein sequence ID" value="ABF09500"/>
        <property type="gene ID" value="Rmet_2623"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2623"/>
    <dbReference type="eggNOG" id="COG1252">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021377_7_0_4"/>
    <dbReference type="OMA" id="ELMGEFI"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003954">
        <property type="term" value="F:NADH dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006116">
        <property type="term" value="P:NADH oxidation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023753">
        <property type="entry name" value="FAD/NAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045024">
        <property type="entry name" value="NDH-2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43706">
        <property type="entry name" value="PTHR43706"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07992">
        <property type="entry name" value="Pyr_redox_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="SSF51905"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="395"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="domain" description="Pyr_redox_2" evidence="4">
        <location>
            <begin position="34"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF07992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABF09500.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="467" mass="50544" checksum="C815CDB8AD253C1B" modified="2006-05-30" version="1">MSHAYDKEDSTQREAATVSTQPRTVARSEKQAHRIIVVGGGAGGLELVTRLGDKLGKSGTAQVTLVDRSPTHIWKPLLHEVAAGSMDPNTHQLEYVAQARWHYFEFQQGELTGIDRERKLVKVAACLDQDGAELLPARELPYDTLVLAIGCVTHFFGVPGAAENAIALDTVDQAERFRRKLIAACVRAQNGRGRVGEDGRPRVDVAIIGAGATGVELSAELRNTAHVLNAYGLHQLDPRRDVRIHIVEAGPRILPALTERVSAETTKLLRKLDVDVFTSERVTEVTKDAVLTASGKHVDADLTVWAAGITAPGVLRTLGLPVSKMGQIVVGPTLQCETDPDIFAFGDCAAAPWPEKQTTVPPRAQAAHQQATFLYDAIRARLAGKPLPKFGFKDFGSLVSLGHFSAVGSLMGGLIGGSMFIEGLFARFMYTSLYRMHVLALHGAIGMGLDTITHWLRSKTSPRVKLH</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="72" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LKB4</accession>
    <name>Q1LKB4_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Extra-cytoplasmic Solute Receptor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">bug</name>
        <name evidence="4" type="ordered locus">Rmet_2535</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Periplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the UPF0065 (bug) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09412.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517111.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2535"/>
    <dbReference type="EnsemblBacteria" id="ABF09412">
        <property type="protein sequence ID" value="ABF09412"/>
        <property type="gene ID" value="Rmet_2535"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2535"/>
    <dbReference type="eggNOG" id="COG3181">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045683_0_2_4"/>
    <dbReference type="OMA" id="AGAYMSK"/>
    <dbReference type="OrthoDB" id="447734at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042597">
        <property type="term" value="C:periplasmic space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005064">
        <property type="entry name" value="BUG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042100">
        <property type="entry name" value="Bug_dom1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42928">
        <property type="entry name" value="PTHR42928"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03401">
        <property type="entry name" value="TctC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017082">
        <property type="entry name" value="YflP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0675">Receptor</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193576" evidence="3">
        <location>
            <begin position="24"/>
            <end position="324"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF09412.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="324" mass="34759" checksum="1F38A2FF5B0C6AFA" modified="2006-05-30" version="1">MIRKRWTCMLALALVLVSQSASAQPTYPTRPISFVLPFSAGGAADTVSRMLAEEMSKRLGQPIIVENRPGAAGVIGTNVAAKAPADGYTVLLTLTQAILTNQFLFTKLPYDPRKELAFVTEVCGGSVLLVVNPRIPAKDVSELLNWAANNRASFGSWGTGSYGHLTSAYLNRSRKIDLTHVAYKGEAPMIQDLVGGQIDIAFVTAFSAKPFIDSGKLRVLAVTGERRLPALPNVPTLAEAGLTDPEFQSTGKLIMMVPAGTPAPIVERIESAARAVIGTEAIRNRMRGIGMEPIGNSSRQALANYEAMYPVHQRLVQLTGAKLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="104" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LKE2</accession>
    <name>Q1LKE2_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Transcriptional regulator, GntR family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Rmet_2507</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">In the C-terminal section; belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09384.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517084.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2507"/>
    <dbReference type="EnsemblBacteria" id="ABF09384">
        <property type="protein sequence ID" value="ABF09384"/>
        <property type="gene ID" value="Rmet_2507"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2507"/>
    <dbReference type="eggNOG" id="COG1167">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017584_0_0_4"/>
    <dbReference type="OMA" id="EGHYRKH"/>
    <dbReference type="OrthoDB" id="1024978at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009058">
        <property type="term" value="P:biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07377">
        <property type="entry name" value="WHTH_GntR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000524">
        <property type="entry name" value="Tscrpt_reg_HTH_GntR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00392">
        <property type="entry name" value="GntR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00345">
        <property type="entry name" value="HTH_GNTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="SSF53383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50949">
        <property type="entry name" value="HTH_GNTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH gntR-type" evidence="3">
        <location>
            <begin position="43"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF09384.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="515" mass="56444" checksum="76F376BF2757161A" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-04-07" version="68" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LKW6</accession>
    <name>Q1LKW6_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Conserved putative exported protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_2333</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09210.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_003098886.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2333"/>
    <dbReference type="EnsemblBacteria" id="ABF09210">
        <property type="protein sequence ID" value="ABF09210"/>
        <property type="gene ID" value="Rmet_2333"/>
    </dbReference>
    <dbReference type="GeneID" id="56409840"/>
    <dbReference type="KEGG" id="rme:Rmet_2333"/>
    <dbReference type="eggNOG" id="COG0695">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_091013_0_0_4"/>
    <dbReference type="OMA" id="DCAPCAQ"/>
    <dbReference type="OrthoDB" id="805554at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022293">
        <property type="entry name" value="Integrating-conj_element"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03759">
        <property type="entry name" value="conj_TIGR03759"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193538" evidence="2">
        <location>
            <begin position="22"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="130"/>
            <end position="149"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF09210.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="245" mass="27314" checksum="86DA12B9069ACA7A" modified="2006-05-30" version="1">MKPSILLFAVLVASSQWPAWAQQPATTSARNAQSQERPLAARVLDDRVATEWGLQPQEWARYRELMDGPLGIYSPNLDPLSALGIEARTDEERRRYAELQVQVEARRVEKLLAYQRAYDEAWQRLNPGMQRVNLPDDKPGAGPSSSPLRGSGRMAVFVKDGCAACGQLVQRLQSSGAEFDLYMVGSRQDDARIRDWAKRAQIDPARVRAGSITLNHDGGRWLSLGLPGDLPAAVREVNGQWQRQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LL70</accession>
    <name>Q1LL70_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Signal transduction protein (GGDEF domain)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Rmet_2227</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09106.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2227"/>
    <dbReference type="EnsemblBacteria" id="ABF09106">
        <property type="protein sequence ID" value="ABF09106"/>
        <property type="gene ID" value="Rmet_2227"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2227"/>
    <dbReference type="eggNOG" id="COG2199">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG5000">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_134_1_4"/>
    <dbReference type="OMA" id="RNVTHER"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01949">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00130">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000160">
        <property type="entry name" value="GGDEF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003660">
        <property type="entry name" value="HAMP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029787">
        <property type="entry name" value="Nucleotide_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000014">
        <property type="entry name" value="PAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035965">
        <property type="entry name" value="PAS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013656">
        <property type="entry name" value="PAS_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00990">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00672">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08448">
        <property type="entry name" value="PAS_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00267">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00304">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00091">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55073">
        <property type="entry name" value="SSF55073"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55785">
        <property type="entry name" value="SSF55785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00254">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00229">
        <property type="entry name" value="sensory_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50887">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50885">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50112">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="280"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="domain" description="HAMP" evidence="3">
        <location>
            <begin position="300"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="domain" description="PAS" evidence="2">
        <location>
            <begin position="365"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="domain" description="GGDEF" evidence="4">
        <location>
            <begin position="518"/>
            <end position="652"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABF09106.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="662" mass="72073" checksum="FD673F9D928D7BB4" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2007-01-23" modified="2022-08-03" version="96" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LLB5</accession>
    <name>PSTB_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Phosphate import ATP-binding protein PstB</fullName>
            <ecNumber evidence="1">7.3.2.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">ABC phosphate transporter</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Phosphate-transporting ATPase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">pstB</name>
        <name type="ordered locus">Rmet_2182</name>
    </gene>
    <organism>
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Part of the ABC transporter complex PstSACB involved in phosphate import. Responsible for energy coupling to the transport system.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + H2O + phosphate(out) = ADP + H(+) + 2 phosphate(in)</text>
            <dbReference type="Rhea" id="RHEA:24440"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="7.3.2.1"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">The complex is composed of two ATP-binding proteins (PstB), two transmembrane proteins (PstC and PstA) and a solute-binding protein (PstS).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell inner membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the ABC transporter superfamily. Phosphate importer (TC 3.A.1.7) family.</text>
    </comment>
    <dbReference type="EC" id="7.3.2.1" evidence="1"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09061.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008641660.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q1LLB5"/>
    <dbReference type="SMR" id="Q1LLB5"/>
    <dbReference type="STRING" id="266264.Rmet_2182"/>
    <dbReference type="EnsemblBacteria" id="ABF09061">
        <property type="protein sequence ID" value="ABF09061"/>
        <property type="gene ID" value="Rmet_2182"/>
    </dbReference>
    <dbReference type="GeneID" id="60821245"/>
    <dbReference type="KEGG" id="rme:Rmet_2182"/>
    <dbReference type="eggNOG" id="COG1117">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_22_4"/>
    <dbReference type="OMA" id="TIDICRV"/>
    <dbReference type="OrthoDB" id="1416748at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015415">
        <property type="term" value="F:ATPase-coupled phosphate ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005315">
        <property type="term" value="F:inorganic phosphate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03260">
        <property type="entry name" value="ABC_PstB_phosphate_transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015850">
        <property type="entry name" value="ABC_transpr_PstB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005670">
        <property type="entry name" value="Phosp_transpt1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43423">
        <property type="entry name" value="PTHR43423"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43423:SF3">
        <property type="entry name" value="PTHR43423:SF3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00972">
        <property type="entry name" value="3a0107s01c2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51238">
        <property type="entry name" value="PSTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0997">Cell inner membrane</keyword>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0592">Phosphate transport</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-1278">Translocase</keyword>
    <keyword id="KW-0813">Transport</keyword>
    <feature type="chain" id="PRO_0000272506" description="Phosphate import ATP-binding protein PstB">
        <location>
            <begin position="1"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="1">
        <location>
            <begin position="16"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="48"/>
            <end position="55"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01702"/>
        </source>
    </evidence>
    <sequence length="262" mass="29736" checksum="7E180307FEB8CDE3" modified="2006-05-30" version="1">MTSTVIDIPDTVRAKIDVRNLNFYYNQFHALKNINMSIPDRKVTAFIGPSGCGKSTLLRTFNKMYALYPEQRAEGEISMDGENLLTSKQDISLLRAKVGMVFQKPTPFPMSIYDNIAFGVRLFEKLSRSEMDDRVEWALSKAALWNEAKDKLHQSGYGLSGGQQQRLCIARGIAIRPEVLLLDEPCSALDPISTGRIEELIAELKDEYTVVIVTHNMQQAARCSDYTAYMYLGELIEFGETEKIFIKPHRKETEDYITGRFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-10-07" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LLD9</accession>
    <name>Q1LLD9_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_2158</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09037.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2158"/>
    <dbReference type="EnsemblBacteria" id="ABF09037">
        <property type="protein sequence ID" value="ABF09037"/>
        <property type="gene ID" value="Rmet_2158"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2158"/>
    <dbReference type="HOGENOM" id="CLU_1433396_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF09037.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="189" mass="21254" checksum="CC71F64509F9C580" modified="2006-05-30" version="1">MSCRDDESEAASSRYAPKPALHLGRIVQDIPPVVHALGKATCRTPDELLASRTRFGYPTYAIEDPDDSVTWYFSNDLQEAVALGLKWLEKDCALIQIPEDQNGEVVYAYVARGLEHDPDLECLLYREGFWRAAGGDMNEAATPEIMTRLRIELFKLLARLGGGQFLLLDFSNPASVTRHLENWENDLEP</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="109" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LLR3</accession>
    <name>Q1LLR3_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Pseudouridine synthase</fullName>
            <ecNumber evidence="3">5.4.99.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Rmet_2034</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
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        <source>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
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        <source>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="EMBL" id="ABF08913.1"/>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
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</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="76" xmlns="http://uniprot.org/uniprot">
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        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
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                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
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                <person name="Monchy S."/>
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                <person name="Taghavi S."/>
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            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
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        <property type="entry name" value="Acrflvin-R"/>
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        </location>
    </feature>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="EMBL" id="ABF08821.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="1036" mass="113168" checksum="ACC9194C0140BEDD" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LM10</accession>
    <name>Q1LM10_CUPMC</name>
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        <submittedName>
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        </submittedName>
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    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
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                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
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                <person name="van der Lelie D."/>
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            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
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        </source>
    </reference>
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    </dbReference>
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    </dbReference>
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        <property type="component" value="Chromosome"/>
    </dbReference>
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        <property type="term" value="F:amidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <location>
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        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="ABF08816.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="396" mass="41530" checksum="7BF27CAD2E554C71" modified="2006-05-30" version="1">MSLATHPSRAFLPYPDAPVPNAANGPLRGLTFAVKDLFDVSGYPTGGGNPHVLARSGIKTTTAPTVQKLLDAGAAFVGKVHTDELAFSMNGQNHHYGGPYNGAAPDRITGGSSSGSASAVSNKLCDFALGTDTGGSVRAPASHCGLFGIRPSHGRISLTHCMPLCETLDTCGFFARDIATFARVADVLLGADANPLPSRPRLLLAADLFELPTPEARKALAPATARIEAVLGKASPVDVADRPLSDLYWAFRYVQGWEAWRADGALIENYGLQLGPDVAGRFAFSKEVTAAQFEKSSAVRREFTAHLGCLLGQDGILLLPTMPDIAPLRTTPMEALEDYRTTAAHTLCLTPLTGFPQLSLPLSGRDGAPLGVSLIGPMGSDRSLIAVAETLMTAIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-12-02" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LM15</accession>
    <name>Q1LM15_CUPMC</name>
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        <submittedName>
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    </protein>
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    </gene>
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        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08811.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1932"/>
    <dbReference type="EnsemblBacteria" id="ABF08811">
        <property type="protein sequence ID" value="ABF08811"/>
        <property type="gene ID" value="Rmet_1932"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1932"/>
    <dbReference type="eggNOG" id="COG0747">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017028_7_4_4"/>
    <dbReference type="OMA" id="TLFTNWR"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039424">
        <property type="entry name" value="SBP_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000914">
        <property type="entry name" value="SBP_5_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30290">
        <property type="entry name" value="PTHR30290"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00496">
        <property type="entry name" value="SBP_bac_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SBP_bac_5" evidence="1">
        <location>
            <begin position="116"/>
            <end position="481"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF08811.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="586" mass="64385" checksum="1F97D307813B2469" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LM18</accession>
    <name>Q1LM18_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Regulatory protein GntR, HTH</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Rmet_1929</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08808.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1929"/>
    <dbReference type="EnsemblBacteria" id="ABF08808">
        <property type="protein sequence ID" value="ABF08808"/>
        <property type="gene ID" value="Rmet_1929"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1929"/>
    <dbReference type="eggNOG" id="COG1802">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017584_5_1_4"/>
    <dbReference type="OMA" id="DETEHWH"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07377">
        <property type="entry name" value="WHTH_GntR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.530">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011711">
        <property type="entry name" value="GntR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008920">
        <property type="entry name" value="TF_FadR/GntR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000524">
        <property type="entry name" value="Tscrpt_reg_HTH_GntR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07729">
        <property type="entry name" value="FCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00392">
        <property type="entry name" value="GntR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00895">
        <property type="entry name" value="FCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00345">
        <property type="entry name" value="HTH_GNTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48008">
        <property type="entry name" value="SSF48008"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50949">
        <property type="entry name" value="HTH_GNTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH gntR-type" evidence="4">
        <location>
            <begin position="42"/>
            <end position="109"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABF08808.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="249" mass="27938" checksum="89B154E1E1F9A526" modified="2006-05-30" version="1">MKYHTSITATSNRFRSAVANPIRLVGAADAEPTIPTPAQSGAAMREHAYTEIKRRIISCEFRPGEPLNEAQVAALLGLGRTPVHQALHRLEVEGLVSILPRKGVLVTPLSLNEVLDMIEVRATNEVLCATLACERGHDSDFKAMRDIVDRSPELIARRDIPALAALDLKFHTAMSAASRNRVLADLLRGLHEKQARFWFLSLSDPQHLENVYQEHLEIVNALERRDVPAVREAIREHIDEFRKNIIRTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="92" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LMF7</accession>
    <name>Q1LMF7_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Glycolate oxidase subunit GlcD</fullName>
            <ecNumber evidence="3">1.1.3.15</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">glcD</name>
        <name evidence="3" type="ordered locus">Rmet_1790</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EC" id="1.1.3.15" evidence="3"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08669.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516519.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1790"/>
    <dbReference type="EnsemblBacteria" id="ABF08669">
        <property type="protein sequence ID" value="ABF08669"/>
        <property type="gene ID" value="Rmet_1790"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1790"/>
    <dbReference type="eggNOG" id="COG0277">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017779_4_1_4"/>
    <dbReference type="OMA" id="TEIAYMK"/>
    <dbReference type="OrthoDB" id="1034540at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003973">
        <property type="term" value="F:(S)-2-hydroxy-acid oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.45.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.43.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.465.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016166">
        <property type="entry name" value="FAD-bd_PCMH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036318">
        <property type="entry name" value="FAD-bd_PCMH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016167">
        <property type="entry name" value="FAD-bd_PCMH_sub1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016169">
        <property type="entry name" value="FAD-bd_PCMH_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016164">
        <property type="entry name" value="FAD-linked_Oxase-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004113">
        <property type="entry name" value="FAD-linked_oxidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006094">
        <property type="entry name" value="Oxid_FAD_bind_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016171">
        <property type="entry name" value="Vanillyl_alc_oxidase_C-sub2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02913">
        <property type="entry name" value="FAD-oxidase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01565">
        <property type="entry name" value="FAD_binding_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55103">
        <property type="entry name" value="SSF55103"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56176">
        <property type="entry name" value="SSF56176"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51387">
        <property type="entry name" value="FAD_PCMH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0274">FAD</keyword>
    <keyword evidence="1" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD-binding PCMH-type" evidence="2">
        <location>
            <begin position="37"/>
            <end position="218"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF08669.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="470" mass="50627" checksum="E9EC7315081B5E2D" modified="2006-05-30" version="1">MSDTNFAQRLTETLGPDVVTTAPDDVAPWLSDWRGLYKGQAQAVIRPRTTEQVAQALALCQQAGVPVVPRGGNTGLCGGAAPDGNQTNVVLSLDRMDKIRSIDTIANTMVVEAGCILGNLRRAAEDADRLFPLSLAAEDSCQIGGNLATNAGGVNVVRYGMTRELVLGVEAVLPNGEIFHGLRTLRKDNTGYDLKQLLIGSEGTLGIITAAALRLLPRADSRAVVLAAVESPKQALELFSLLFARCGARLQAYEFFTGACLDLVLTHVEGIHAPFAERYPAYVLVEIADTAPEDVLNALLESVIGDALEQGLCLDAAVSASLAQLQSLWRLREEISEAQRADGLHLKHDVSLPIEAIPDFMTSMETRLAARHPGVRPFIFGHFGDGNLHYNLSRPLGADPKWALNEGKAVSEDVLEEVARYDGSISAEHGIGQLKRESFLALKDPLELRLMRAIKQVFDPSGIMNPGKLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="102" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LMG5</accession>
    <name>Q1LMG5_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Phenol hydroxylase P5 protein (Phenol 2-monooxygenase P5 component)</fullName>
            <ecNumber evidence="7">1.14.13.7</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">phyE</name>
        <name evidence="7" type="ordered locus">Rmet_1782</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EC" id="1.14.13.7" evidence="7"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08661.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516514.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1782"/>
    <dbReference type="EnsemblBacteria" id="ABF08661">
        <property type="protein sequence ID" value="ABF08661"/>
        <property type="gene ID" value="Rmet_1782"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1782"/>
    <dbReference type="eggNOG" id="COG2871">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003827_7_0_4"/>
    <dbReference type="OMA" id="DVMRIRL"/>
    <dbReference type="OrthoDB" id="1834577at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018662">
        <property type="term" value="F:phenol 2-monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd00207">
        <property type="entry name" value="fer2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036010">
        <property type="entry name" value="2Fe-2S_ferredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001041">
        <property type="entry name" value="2Fe-2S_ferredoxin-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006058">
        <property type="entry name" value="2Fe2S_fd_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012675">
        <property type="entry name" value="Beta-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008333">
        <property type="entry name" value="Cbr1-like_FAD-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017927">
        <property type="entry name" value="FAD-bd_FR_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001709">
        <property type="entry name" value="Flavoprot_Pyr_Nucl_cyt_Rdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039261">
        <property type="entry name" value="FNR_nucleotide-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001433">
        <property type="entry name" value="OxRdtase_FAD/NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017938">
        <property type="entry name" value="Riboflavin_synthase-like_b-brl"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00970">
        <property type="entry name" value="FAD_binding_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00111">
        <property type="entry name" value="Fer2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00175">
        <property type="entry name" value="NAD_binding_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00371">
        <property type="entry name" value="FPNCR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52343">
        <property type="entry name" value="SSF52343"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54292">
        <property type="entry name" value="SSF54292"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63380">
        <property type="entry name" value="SSF63380"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00197">
        <property type="entry name" value="2FE2S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51085">
        <property type="entry name" value="2FE2S_FER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51384">
        <property type="entry name" value="FAD_FR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0001">2Fe-2S</keyword>
    <keyword evidence="3" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="2Fe-2S ferredoxin-type" evidence="5">
        <location>
            <begin position="3"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="domain" description="FAD-binding FR-type" evidence="6">
        <location>
            <begin position="102"/>
            <end position="205"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABF08661.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="356" mass="38807" checksum="B6AAE5E015104A71" modified="2006-05-30" version="1">MTYQLTIEPLGQTIDIAEGQTVLDACLRAGVWLPHACGHGLCGTCKVQTVDGEIEHGEASPFALMDFEREEAKCLACCATPMSDLTIEADIDPEPDAQFLPLMDFTAEVTRIEALTPTIKGVFLRVEGVEGGDALRFQPGQYVNVWIGKEATPRAFSIASAPSADEIELNIRLVPGGSATTYVHEQLAVGERLQLSGPLGRFFVRKSDPRPLVFMAGGSGLSSPRSMILDLLESGDQRQIVLVHGARTADELYYRDAFEALTTRHDNFTYVPVLSAEAQGSAWAGERGYVHEAAGSHFGYDFRGWRAYLCGPPPMIESCIAALMQGRLFEKDIFTEKFLSAADASHATRSPLFRSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="63" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LMH6</accession>
    <name>Q1LMH6_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_1771</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08650.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516503.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1771"/>
    <dbReference type="EnsemblBacteria" id="ABF08650">
        <property type="protein sequence ID" value="ABF08650"/>
        <property type="gene ID" value="Rmet_1771"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1771"/>
    <dbReference type="eggNOG" id="ENOG50344T9">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_133655_0_0_4"/>
    <dbReference type="OMA" id="YYYDPWY"/>
    <dbReference type="OrthoDB" id="1868651at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF08650.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="147" mass="15061" checksum="F11A58B28ABB38C1" modified="2006-05-30" version="1">MGKGLAAVAAAIAGLGLGGGVGGAWAQDMSTMCQQVVGQAEIGGVMQTIAGVACLQPDGTWQMVDSSSVGYGGGDYYYDPWYWTPYVGFAGVIFIDHHHRPHVMHHVFFPSRGVIVRGGRVVHGGAPGGFHGGAAPGGFHGGGAMHR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="72" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LMP5</accession>
    <name>Q1LMP5_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_1700</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08581.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516433.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1700"/>
    <dbReference type="EnsemblBacteria" id="ABF08581">
        <property type="protein sequence ID" value="ABF08581"/>
        <property type="gene ID" value="Rmet_1700"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1700"/>
    <dbReference type="eggNOG" id="ENOG5034AC1">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2506310_0_0_4"/>
    <dbReference type="OMA" id="FWKQRKV"/>
    <dbReference type="OrthoDB" id="2026769at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="SSF54928"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF08581.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="84" mass="9733" checksum="1229F5ED830C5A10" modified="2006-05-30" version="1">MALLMISNLNPDTTEDQVKEFLGRYGFPAYDEFELQEGDGTRPAVTLTFHAVDETALQHFVPRIHDVFWNGHRINALVLRERFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-12-02" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LMS7</accession>
    <name>Q1LMS7_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Transposase (DDE site) ISRme7</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">tnp</name>
        <name evidence="3" type="ordered locus">Rmet_1668</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08549.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1668"/>
    <dbReference type="EnsemblBacteria" id="ABF08549">
        <property type="protein sequence ID" value="ABF08549"/>
        <property type="gene ID" value="Rmet_1668"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1668"/>
    <dbReference type="eggNOG" id="COG3316">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_813469_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032874">
        <property type="entry name" value="DDE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13610">
        <property type="entry name" value="DDE_Tnp_IS240"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="SSF53098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DDE_Tnp_IS240" evidence="2">
        <location>
            <begin position="80"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="232"/>
            <end position="262"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13610"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF08549.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="341" mass="38969" checksum="3EB1ABCA19A0F882" modified="2006-05-30" version="1">MIKELPAGVAKVLKRLHHPLDVILLCVRWYVAYSLSLRDLEEMMAERGLAVDHSMVHRWVIKLLPLFEKAFRRHKRSVSKSWRMDETYLKVRGKWAYLYRAVDKAGNTIDFLLCARRDKVAARRYFEKAIGQNGAPDTVAIDKSAANLAALHAVNAHRETPIRIRQRKYLNNIVEQDHRAIKRRARPMLGFKNFRCARILIGGIETMHMIAKGRCGDPKAFACPPRSNSIPWFHRPAHSSPPASTNRPYRDRTKGAGAPAFGIQTPFDSSRGSLLLQWQARLMIFDALSPTSRFHSVPEAMHLGKVVVDRGFRRPQSSLRVDTASYRINLAGERHVSRTHQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="53" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LN17</accession>
    <name>Q1LN17_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_1576</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08459.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516311.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1576"/>
    <dbReference type="EnsemblBacteria" id="ABF08459">
        <property type="protein sequence ID" value="ABF08459"/>
        <property type="gene ID" value="Rmet_1576"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1576"/>
    <dbReference type="eggNOG" id="ENOG5032ZZK">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_117104_0_0_4"/>
    <dbReference type="OrthoDB" id="2090294at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF08459.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="227" mass="24068" checksum="928DA32D99A93D60" modified="2006-05-30" version="1">MVDPIFRKTEAGQEEIRTRERKLDQKLRALLLIVNGERARSELVAQVGALGMAGEALDTLLALGLIEPIPAPAGAKPALSTVPATVRPTTGPARPAPQRAPAETNLFAAYSSFNTSDGKGIDDVLADTTIAPGALPAAKPSTQPATPAPDSYQRLYHFFTDVIGNHLGLRGYMLQVKVEKAATLVELAALRDMLGAALQKAKGEITARAIIDQLDQLLAEMGMAPVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LN20</accession>
    <name>Q1LN20_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">tRNA-specific adenosine deaminase</fullName>
            <ecNumber evidence="3">3.5.4.33</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3 5" type="primary">tadA</name>
        <name evidence="5" type="ordered locus">Rmet_1573</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Catalyzes the deamination of adenosine to inosine at the wobble position 34 of tRNA(Arg2).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>adenosine(34) in tRNA + H(+) + H2O = inosine(34) in tRNA + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:43168"/>
            <dbReference type="Rhea" id="RHEA-COMP:10373"/>
            <dbReference type="Rhea" id="RHEA-COMP:10374"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:74411"/>
            <dbReference type="ChEBI" id="CHEBI:82852"/>
            <dbReference type="EC" id="3.5.4.33"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="3">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the cytidine and deoxycytidylate deaminase family.</text>
    </comment>
    <dbReference type="EC" id="3.5.4.33" evidence="3"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08456.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516308.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1573"/>
    <dbReference type="EnsemblBacteria" id="ABF08456">
        <property type="protein sequence ID" value="ABF08456"/>
        <property type="gene ID" value="Rmet_1573"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1573"/>
    <dbReference type="eggNOG" id="COG0590">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025810_3_0_4"/>
    <dbReference type="OMA" id="PCQMCAG"/>
    <dbReference type="OrthoDB" id="1890883at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052717">
        <property type="term" value="F:tRNA-specific adenosine-34 deaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002100">
        <property type="term" value="P:tRNA wobble adenosine to inosine editing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00972">
        <property type="entry name" value="tRNA_aden_deaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016192">
        <property type="entry name" value="APOBEC/CMP_deaminase_Zn-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002125">
        <property type="entry name" value="CMP_dCMP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016193">
        <property type="entry name" value="Cytidine_deaminase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028883">
        <property type="entry name" value="tRNA_aden_deaminase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14437">
        <property type="entry name" value="MafB19-deam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53927">
        <property type="entry name" value="SSF53927"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00903">
        <property type="entry name" value="CYT_DCMP_DEAMINASES_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51747">
        <property type="entry name" value="CYT_DCMP_DEAMINASES_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1 3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0819">tRNA processing</keyword>
    <keyword evidence="2 3" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="CMP/dCMP-type deaminase" evidence="4">
        <location>
            <begin position="12"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="3">
        <location>
            <position position="65"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="63"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="93"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="96"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00972"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABF08456.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="189" mass="20254" checksum="37D3A9FB5130C651" modified="2006-05-30" version="1">MADVPVFADPAERDRFYMAAALDEARLAEAAGEVPVGAVVVWDDKIIARGHNLPIRSVDPSAHAEMQALRAAAKVLGNYRMPECEIYVTLEPCPMCSGAILHARLRHVVFGATDPKTGAAGSVVDLFAQATLNHQTTLTRGVMADECGQLLRDFFGARRRAQKAARALQVSPVDADSGKIQASDPNSET</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LN25</accession>
    <name>Q1LN25_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_1568</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08451.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516305.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1568"/>
    <dbReference type="EnsemblBacteria" id="ABF08451">
        <property type="protein sequence ID" value="ABF08451"/>
        <property type="gene ID" value="Rmet_1568"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1568"/>
    <dbReference type="eggNOG" id="COG5002">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_144643_0_0_4"/>
    <dbReference type="OMA" id="EFTIQML"/>
    <dbReference type="OrthoDB" id="1755994at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00130">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001610">
        <property type="entry name" value="PAC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000014">
        <property type="entry name" value="PAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000700">
        <property type="entry name" value="PAS-assoc_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035965">
        <property type="entry name" value="PAS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013767">
        <property type="entry name" value="PAS_fold"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00989">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00086">
        <property type="entry name" value="PAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00091">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55785">
        <property type="entry name" value="SSF55785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00229">
        <property type="entry name" value="sensory_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50113">
        <property type="entry name" value="PAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50112">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PAS" evidence="1">
        <location>
            <begin position="4"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="domain" description="PAC" evidence="2">
        <location>
            <begin position="81"/>
            <end position="133"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF08451.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="139" mass="14861" checksum="7AF2731C8E01682E" modified="2006-05-30" version="1">MTISTNTAAQILEQLADALIFADTDGRITGWNHAAAELFGYGPDEALGQSLDLIIPERLQAAHWKGFQAAIDTGKTRLSGKPTLTKAIHKDGRKLFVEMTFALISDANGNVIGSVAVARDVTDKVERERAQSAAGPVSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LN57</accession>
    <name>Q1LN57_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">GTP hydrolase involved in nickel liganding into hydrogenases</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">hypB2</name>
        <name evidence="3" type="ordered locus">Rmet_1536</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SIMIBI class G3E GTPase family. HypB/HupM subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08419.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516276.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1536"/>
    <dbReference type="EnsemblBacteria" id="ABF08419">
        <property type="protein sequence ID" value="ABF08419"/>
        <property type="gene ID" value="Rmet_1536"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1536"/>
    <dbReference type="eggNOG" id="COG0378">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_056148_1_0_4"/>
    <dbReference type="OMA" id="GRSCHLD"/>
    <dbReference type="OrthoDB" id="1134430at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016151">
        <property type="term" value="F:nickel cation binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051604">
        <property type="term" value="P:protein maturation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05390">
        <property type="entry name" value="HypB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003495">
        <property type="entry name" value="CobW/HypB/UreG_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004392">
        <property type="entry name" value="Hyd_mat_HypB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30134">
        <property type="entry name" value="PTHR30134"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02492">
        <property type="entry name" value="cobW"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00073">
        <property type="entry name" value="hypB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="cobW" evidence="2">
        <location>
            <begin position="115"/>
            <end position="275"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF08419.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="326" mass="35917" checksum="95DA1A745F4FB8EA" modified="2006-05-30" version="1">MCTICGCGAGETRIEGQELEHTHEHVHADGTVHAHAHPPHEQDHHYADHDHDHAHAHRAVRGTDHLHYGHGPAGAHAPGMSQARMVKIEQDILGKNNAYAAQNRRWFDEHGVFALNFVSSPGSGKTTLLVRTIEALQSTCGLAVIEGDQQTSFDAERIRATGVRALQINTGKGCHLDAYMVGHALEQLRPQDESVLLIENVGNLVCPSAFDLGEAHKVVILSVTEGEDKPLKYPDMFRAASLMLLNKCDLLPHLSFDVERTIEYARRVNPALHVIRTSSTTGEGFAEWLAWITEGLVGQRAKRSQNVDLLRARVAELEAELAVLKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LN76</accession>
    <name>Q1LN76_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">cbbY</name>
        <name evidence="1" type="ordered locus">Rmet_1517</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08400.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516260.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1517"/>
    <dbReference type="EnsemblBacteria" id="ABF08400">
        <property type="protein sequence ID" value="ABF08400"/>
        <property type="gene ID" value="Rmet_1517"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1517"/>
    <dbReference type="eggNOG" id="COG0637">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045011_0_2_4"/>
    <dbReference type="OMA" id="KENGWPT"/>
    <dbReference type="OrthoDB" id="1167152at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.240">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044999">
        <property type="entry name" value="CbbY-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006439">
        <property type="entry name" value="HAD-SF_hydro_IA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041492">
        <property type="entry name" value="HAD_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023198">
        <property type="entry name" value="PGP-like_dom2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42896">
        <property type="entry name" value="PTHR42896"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13419">
        <property type="entry name" value="HAD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00413">
        <property type="entry name" value="HADHALOGNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="SSF56784"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01509">
        <property type="entry name" value="HAD-SF-IA-v3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF08400.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="272" mass="29726" checksum="F29DF93208B30DAC" modified="2006-05-30" version="1">MTTGTAARPKLEALLWDVDGTLAETERDGHRVAFNLAFEAFGLPWRWDDARYGELLRVTGGRERLMHDMDSRPDAPPMASERDALARALHAKKNTLYAELVADAGIPLRDGVLELMRECRDHGVRMAVATTTSRSNLEALLRKHMGNRWAGWFGAIVCGEDVERKKPDPEVFVKALRALGVGPHQAVAIEDSPGGVAAARAADCPVVVTRSFYFAKAPIEGAIAVGPGLHAREGWRPALAARVKPPQSQGQRVGLADIADWFAQRDTICDYP</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="109" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LN78</accession>
    <name>Q1LN78_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Glyceraldehyde-3-phosphate dehydrogenase</fullName>
            <ecNumber evidence="8">1.2.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">cbbG1</name>
        <name evidence="10" type="ordered locus">Rmet_1515</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the glyceraldehyde-3-phosphate dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.2.1.-" evidence="8"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08398.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516258.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1515"/>
    <dbReference type="EnsemblBacteria" id="ABF08398">
        <property type="protein sequence ID" value="ABF08398"/>
        <property type="gene ID" value="Rmet_1515"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1515"/>
    <dbReference type="eggNOG" id="COG0057">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030140_0_3_4"/>
    <dbReference type="OMA" id="NCVAPMA"/>
    <dbReference type="OrthoDB" id="944149at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016620">
        <property type="term" value="F:oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006006">
        <property type="term" value="P:glucose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020831">
        <property type="entry name" value="GlycerAld/Erythrose_P_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020830">
        <property type="entry name" value="GlycerAld_3-P_DH_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020829">
        <property type="entry name" value="GlycerAld_3-P_DH_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020828">
        <property type="entry name" value="GlycerAld_3-P_DH_NAD(P)-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006424">
        <property type="entry name" value="Glyceraldehyde-3-P_DH_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10836">
        <property type="entry name" value="PTHR10836"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02800">
        <property type="entry name" value="Gp_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00044">
        <property type="entry name" value="Gp_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000149">
        <property type="entry name" value="GAP_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00078">
        <property type="entry name" value="G3PDHDRGNASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00846">
        <property type="entry name" value="Gp_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01534">
        <property type="entry name" value="GAPDH-I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00071">
        <property type="entry name" value="GAPDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0520">NAD</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="2 8 10" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Gp_dh_N" evidence="9">
        <location>
            <begin position="3"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="region of interest" description="Glyceraldehyde 3-phosphate binding" evidence="4">
        <location>
            <begin position="151"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="region of interest" description="Glyceraldehyde 3-phosphate binding" evidence="4">
        <location>
            <begin position="211"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="3">
        <location>
            <position position="152"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="12"/>
            <end position="13"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="36"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="122"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="182"/>
        </location>
        <ligand>
            <name>D-glyceraldehyde 3-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="234"/>
        </location>
        <ligand>
            <name>D-glyceraldehyde 3-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="316"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="site" description="Activates thiol group during catalysis" evidence="6">
        <location>
            <position position="179"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR000149-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR000149-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR000149-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR000149-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000397"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU361160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ABF08398.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="335" mass="36026" checksum="1472A382DFDAA6B1" modified="2006-05-30" version="1">MTIRIGINGFGRIGRMVFRAAVQDFKNDIEVVAINDLLEPDYLAYMLRYDSVHGRFKGEVVVDGGTLVVNGRKIRLTAVKDPAALAWGDVGVDVVVESTGLFLTHDTCQKHLDAGARKVIQSAPSKDDTPMFVYGVNHTQYAGEKIVSNASCTTNCLAPVAKVLNDSFGIKRGMMTTVHAATATQKTVDGPSNKDWRGGRGILENIIPSSTGAAKAMGRVIPALNNKLTGMALRVPTSDVSVVDLTVELAQETSYEKICAAMKAASQGAMKGVLGYTQDKVVSTDFRGEACTAVFDAEAGIMLDSTFVKVVAWYDNEWGYSSKVLEMVRVMGASR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LNZ4</accession>
    <name>Q1LNZ4_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">GCN5-related N-acetyltransferase, GNAT family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_1246</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08132.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516018.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1246"/>
    <dbReference type="EnsemblBacteria" id="ABF08132">
        <property type="protein sequence ID" value="ABF08132"/>
        <property type="gene ID" value="Rmet_1246"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1246"/>
    <dbReference type="eggNOG" id="COG3153">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_081840_2_0_4"/>
    <dbReference type="OMA" id="TRCHIGD"/>
    <dbReference type="OrthoDB" id="1687413at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008080">
        <property type="term" value="F:N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="SSF55729"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="1">
        <location>
            <begin position="3"/>
            <end position="154"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF08132.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="172" mass="18455" checksum="F9EF0D9F3B163E58" modified="2006-05-30" version="1">MTLQLRHETPDDIAAIEAVTIAAFADAPHTSHTEQFIVRALRAANELTLSIVAEEHGRVVGHVALSPVTITHEHGRKTEGWYRLGPISVLPPRQGQGIGSRLMEQALSELRAMQAAGCVLLGDPTYYTRFGFQAHAGLQLPGVPPGYFMALALHGTVPEGIAHYSDAFNAAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LP28</accession>
    <name>Q1LP28_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative oxidoreductase, NADP(H)-dependent aldo-keto reductase</fullName>
            <ecNumber evidence="2">1.1.1.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">tas</name>
        <name evidence="2" type="ordered locus">Rmet_1212</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EC" id="1.1.1.-" evidence="2"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08098.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515988.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1212"/>
    <dbReference type="EnsemblBacteria" id="ABF08098">
        <property type="protein sequence ID" value="ABF08098"/>
        <property type="gene ID" value="Rmet_1212"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1212"/>
    <dbReference type="eggNOG" id="COG0667">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023205_2_0_4"/>
    <dbReference type="OMA" id="PPYSLFW"/>
    <dbReference type="OrthoDB" id="1351747at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023210">
        <property type="entry name" value="NADP_OxRdtase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036812">
        <property type="entry name" value="NADP_OxRdtase_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00248">
        <property type="entry name" value="Aldo_ket_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51430">
        <property type="entry name" value="SSF51430"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aldo_ket_red" evidence="1">
        <location>
            <begin position="15"/>
            <end position="343"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF08098.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="352" mass="39192" checksum="88F6834F43FEFA4A" modified="2006-05-30" version="1">MKMIRLGRSDLEVSRICLGTMTFGEQNTEAEGHSQLDYAFSRGINFIDTAEMYPVKPRAETYGSTERIIGTWLKKQPRDRVVLASKVAGPARNPWIRNGGDLTPDSIRAAVEDSLKRLQTDYIDLYQIHWPARNAPIFGQKRFNPEAERQCATIEAQLEAMGDLVRAGKIRYVGVSNETPWGVTEFVNVAERHGLPRIATIQNPYNLVNRSFEQGLDETCFRTEVSLLAYSPLGFGQLTGKYLTGDANHPVFNPDAVGRLTRFSPDWSPRYMRAETMAAAARYLELARAHGMSGATLALAWCYSRWFIASTIIGATGLAQLKENVDALDTALSAEVLSAVERIHAEIHNPAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LP39</accession>
    <name>Q1LP39_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Histone deacetylase superfamily</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_1201</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the histone deacetylase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08087.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1201"/>
    <dbReference type="EnsemblBacteria" id="ABF08087">
        <property type="protein sequence ID" value="ABF08087"/>
        <property type="gene ID" value="Rmet_1201"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1201"/>
    <dbReference type="eggNOG" id="COG0123">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007727_1_0_4"/>
    <dbReference type="OMA" id="RVFTFSM"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004407">
        <property type="term" value="F:histone deacetylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016575">
        <property type="term" value="P:histone deacetylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd09993">
        <property type="entry name" value="HDAC_classIV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.800.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044150">
        <property type="entry name" value="HDAC_classIV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000286">
        <property type="entry name" value="His_deacetylse"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023801">
        <property type="entry name" value="His_deacetylse_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037138">
        <property type="entry name" value="His_deacetylse_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023696">
        <property type="entry name" value="Ureohydrolase_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00850">
        <property type="entry name" value="Hist_deacetyl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01270">
        <property type="entry name" value="HDASUPER"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52768">
        <property type="entry name" value="SSF52768"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Hist_deacetyl" evidence="2">
        <location>
            <begin position="56"/>
            <end position="302"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF08087.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="323" mass="34874" checksum="237EC269A2EA5DA2" modified="2006-05-30" version="1">MMSFRHFPGRSSCPIPMLAFYADHFVLPLPPGHRFPMRKYSMLRDAVVREVGGVELQEAPRADDATLALAHTAAYIEDVSTGQLDAARQREIGFPWSHEMVERSRRSAGATIAACRVALEQGIAANLAGGTHHAYADKGAGFCVFNDAAIAARRLQRDGSVRRVAVIDLDVHQGNGTASILRDDPTIFTLSLHGEKNYPFRKEASDLDVGLPDGCDDGTYAVALASALETLFARFDPDLLIYLAGADPHEGDRLGRLKLTMAGLAQRDSMVFEAARSRGLPVAVAMAGGYGNQIEDTVAVHTQTIMLAARYHAQLAAERASVS</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-01-15" modified="2022-08-03" version="95" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LP76</accession>
    <name>SYFA_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Phenylalanine--tRNA ligase alpha subunit</fullName>
            <ecNumber evidence="1">6.1.1.20</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Phenylalanyl-tRNA synthetase alpha subunit</fullName>
            <shortName evidence="1">PheRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">pheS</name>
        <name type="ordered locus">Rmet_1164</name>
    </gene>
    <organism>
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + L-phenylalanine + tRNA(Phe) = AMP + diphosphate + H(+) + L-phenylalanyl-tRNA(Phe)</text>
            <dbReference type="Rhea" id="RHEA:19413"/>
            <dbReference type="Rhea" id="RHEA-COMP:9668"/>
            <dbReference type="Rhea" id="RHEA-COMP:9699"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58095"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78531"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.20"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="1">Binds 2 magnesium ions per tetramer.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Tetramer of two alpha and two beta subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-II aminoacyl-tRNA synthetase family. Phe-tRNA synthetase alpha subunit type 1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.20" evidence="1"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08050.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515946.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q1LP76"/>
    <dbReference type="SMR" id="Q1LP76"/>
    <dbReference type="STRING" id="266264.Rmet_1164"/>
    <dbReference type="EnsemblBacteria" id="ABF08050">
        <property type="protein sequence ID" value="ABF08050"/>
        <property type="gene ID" value="Rmet_1164"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1164"/>
    <dbReference type="eggNOG" id="COG0016">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025086_0_1_4"/>
    <dbReference type="OMA" id="DWHNFTA"/>
    <dbReference type="OrthoDB" id="469058at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004826">
        <property type="term" value="F:phenylalanine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006432">
        <property type="term" value="P:phenylalanyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00281">
        <property type="entry name" value="Phe_tRNA_synth_alpha1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004529">
        <property type="entry name" value="Phe-tRNA-synth_IIc_asu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004530">
        <property type="entry name" value="Phe-tRNA-synth_IIc_mito"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004188">
        <property type="entry name" value="Phe-tRNA_ligase_II_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022911">
        <property type="entry name" value="Phe_tRNA_ligase_alpha1_bac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002319">
        <property type="entry name" value="Phenylalanyl-tRNA_Synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010978">
        <property type="entry name" value="tRNA-bd_arm"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11538:SF41">
        <property type="entry name" value="PTHR11538:SF41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02912">
        <property type="entry name" value="Phe_tRNA-synt_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01409">
        <property type="entry name" value="tRNA-synt_2d"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46589">
        <property type="entry name" value="SSF46589"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="SSF55681"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00468">
        <property type="entry name" value="pheS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_1000006879" description="Phenylalanine--tRNA ligase alpha subunit">
        <location>
            <begin position="1"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="268"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <note>shared with beta subunit</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00281"/>
        </source>
    </evidence>
    <sequence length="343" mass="38517" checksum="A7918A601E517FF3" modified="2006-05-30" version="1">MSLDLDQVVADAQAAFAAANDNATLENEKARFLGKTGALTELLKGLGKLDPDARKTEGARINQVKQQVEAALQARRQALADALMNARLAAEAIDVTLPGRSVARGSVHPVMRTWERVEQIFGSIGFDVADGPEIETDWMNFTALNNPDNHPARSMQDTFYVDGRDSDDKLLLLRTHTSPMQVRYARMHVEKYAGRAMPPIKVICPGRTYRVDSDATHSPMFNQVEGLWIGDNVSFADLKGVYTDFLRKFFERDDIQVRFRPSYFPFTEPSAEIDMAFGNGKWLEISGSGQVHPNVLRNMGLDPERYIGFAFGSGLERLTMLRYGINDLRLFFEGDVRFLRQFA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="103" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LPB0</accession>
    <name>Q1LPB0_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Multimeric flavodoxin WrbA</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">wrbA</name>
        <name evidence="6" type="ordered locus">Rmet_1130</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the WrbA family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08016.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1130"/>
    <dbReference type="EnsemblBacteria" id="ABF08016">
        <property type="protein sequence ID" value="ABF08016"/>
        <property type="gene ID" value="Rmet_1130"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1130"/>
    <dbReference type="eggNOG" id="COG0655">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_051402_0_2_4"/>
    <dbReference type="OMA" id="SRFGMMA"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003955">
        <property type="term" value="F:NAD(P)H dehydrogenase (quinone) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008254">
        <property type="entry name" value="Flavodoxin/NO_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001226">
        <property type="entry name" value="Flavodoxin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029039">
        <property type="entry name" value="Flavoprotein-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010089">
        <property type="entry name" value="Flavoprotein_WrbA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005025">
        <property type="entry name" value="FMN_Rdtase-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03358">
        <property type="entry name" value="FMN_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52218">
        <property type="entry name" value="SSF52218"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01755">
        <property type="entry name" value="flav_wrbA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00201">
        <property type="entry name" value="FLAVODOXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50902">
        <property type="entry name" value="FLAVODOXIN_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="4" id="KW-0288">FMN</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Flavodoxin-like" evidence="5">
        <location>
            <begin position="16"/>
            <end position="200"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABF08016.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="209" mass="22164" checksum="18F0717BA08C95A0" modified="2006-05-30" version="1">MHLRRPRPANPSMTDILVLYYSRHGATRKLAELIARGIDSVPGAQSRLRTVPPVSTVCEATAPDIPPEGAPYAELRDLEECAGLALGSPTRFGNMAAPMKYFLDGTVAQWLSGALTGKPACVFTSTGSLHGGQETTLLSMMLPLLHHGMLIVGLPYAESGLMTTTAGGTPYGPSHYAHVDNQGPITEQEASLATAMGRRLAETALKLAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LPP8</accession>
    <name>Q1LPP8_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Nitrilase</fullName>
            <ecNumber evidence="3">3.5.5.1</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">nit</name>
        <name evidence="3" type="ordered locus">Rmet_0992</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EC" id="3.5.5.1" evidence="3"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07878.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515793.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0992"/>
    <dbReference type="EnsemblBacteria" id="ABF07878">
        <property type="protein sequence ID" value="ABF07878"/>
        <property type="gene ID" value="Rmet_0992"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0992"/>
    <dbReference type="eggNOG" id="COG0388">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030130_1_2_4"/>
    <dbReference type="OMA" id="GNTYRES"/>
    <dbReference type="OrthoDB" id="1650683at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016811">
        <property type="term" value="F:hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080061">
        <property type="term" value="F:indole-3-acetonitrile nitrilase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006807">
        <property type="term" value="P:nitrogen compound metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07572">
        <property type="entry name" value="nit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.110.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003010">
        <property type="entry name" value="C-N_Hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036526">
        <property type="entry name" value="C-N_Hydrolase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045254">
        <property type="entry name" value="Nit1/2_C-N_Hydrolase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00795">
        <property type="entry name" value="CN_hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56317">
        <property type="entry name" value="SSF56317"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50263">
        <property type="entry name" value="CN_HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CN hydrolase" evidence="2">
        <location>
            <begin position="7"/>
            <end position="255"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF07878.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="273" mass="30052" checksum="488ECE9F321D4CCD" modified="2006-05-30" version="1">MTATAPMRVAAIQTVTGITLDDNLARADALIAEAARGGAELVLLPEYFCMMGRHETDKVAIREQDGDGPVQSFLADAARRHRVWLVGGTLPMWCNDDARVYNTSLAFDPHGRRVARYDKIHLFGFTKGTESYDESRTILAGKTPVAFDAPCGRVAMSVCYDLRFPELYRGLAGKNDVSLILMPAAFTYTTGQAHWEILLRARAIENQCYVLAAAQGGKHENGRRTWGHSMLVDPWGELMGVLPEGEGIVSGTVDPTRLAEVRQNLPALRHRVL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-11-28" modified="2022-05-25" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LPR2</accession>
    <name>Y978_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">UPF0246 protein Rmet_0978</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">Rmet_0978</name>
    </gene>
    <organism>
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0246 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07864.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515782.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q1LPR2"/>
    <dbReference type="SMR" id="Q1LPR2"/>
    <dbReference type="STRING" id="266264.Rmet_0978"/>
    <dbReference type="EnsemblBacteria" id="ABF07864">
        <property type="protein sequence ID" value="ABF07864"/>
        <property type="gene ID" value="Rmet_0978"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0978"/>
    <dbReference type="eggNOG" id="COG3022">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_061989_0_0_4"/>
    <dbReference type="OMA" id="WKNGQYK"/>
    <dbReference type="OrthoDB" id="1361974at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00652">
        <property type="entry name" value="UPF0246"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005583">
        <property type="entry name" value="YaaA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30283">
        <property type="entry name" value="PTHR30283"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03883">
        <property type="entry name" value="H2O2_YaaD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000262047" description="UPF0246 protein Rmet_0978">
        <location>
            <begin position="1"/>
            <end position="261"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00652"/>
        </source>
    </evidence>
    <sequence length="261" mass="29645" checksum="B0300674CFA58886" modified="2006-05-30" version="1">MLIVLSPAKSLDYETPPRVKSHTLPRFIDRSATLIERLRKLAPQDVASLMDISDKLAVLNVTRYADWSPEFTAANSKQAVLAFNGDVYDGLDAKTLSTDDLAFAQKHIRILSGLYGVLRPMDWMQPYRLEMGTRLDNAAGKDLYAFWGDDVTALINKDMAELKHEGSLTLVNLASEEYFKVVRPKVLAARVITPVFEDWKGGRYKIISFHAKRARGTMARYAVTHRVTDPAQLKRFNEDGYAFDKAASDDARWVFRRRLED</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LPY8</accession>
    <name>Q1LPY8_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Cytokinin riboside 5'-monophosphate phosphoribohydrolase</fullName>
            <ecNumber evidence="2">3.2.2.n1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_0902</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>AMP + H2O = adenine + D-ribose 5-phosphate</text>
            <dbReference type="Rhea" id="RHEA:20129"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16708"/>
            <dbReference type="ChEBI" id="CHEBI:78346"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="3.2.2.4"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the LOG family.</text>
    </comment>
    <dbReference type="EC" id="3.2.2.n1" evidence="2"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07788.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515728.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0902"/>
    <dbReference type="EnsemblBacteria" id="ABF07788">
        <property type="protein sequence ID" value="ABF07788"/>
        <property type="gene ID" value="Rmet_0902"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0902"/>
    <dbReference type="eggNOG" id="COG1611">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_058336_4_2_4"/>
    <dbReference type="OMA" id="HQKPIGL"/>
    <dbReference type="OrthoDB" id="1255127at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008714">
        <property type="term" value="F:AMP nucleosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009691">
        <property type="term" value="P:cytokinin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005269">
        <property type="entry name" value="LOG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031100">
        <property type="entry name" value="LOG_fam"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03641">
        <property type="entry name" value="Lysine_decarbox"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00730">
        <property type="entry name" value="TIGR00730"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0203">Cytokinin biosynthesis</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0456">Lyase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU363015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF07788.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="194" mass="20983" checksum="157F679C46B188E0" modified="2006-05-30" version="1">MKSVCVYCGSSPGFRPEYAEAARALGQAMAERGLALVYGGGNVGLMGIVADSVMAHGGAAIGIIPEALMQKEVGHRGLTELHIVRNMHERKQMMADRADAFIAMPGGVGTFEELFETFTWAQLGYHDKPVGLLNVAGFYDGMLGFLNHAVSEGFLKQVHADMLHVSTEPEDMLARLAAAPRVHVDKWQEGREKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LQF8</accession>
    <name>Q1LQF8_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Membrane-bound lytic murein transglycosylase B</fullName>
            <ecNumber evidence="3">3.2.1.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">mltB</name>
        <name evidence="3" type="ordered locus">Rmet_0732</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EC" id="3.2.1.-" evidence="3"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07618.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0732"/>
    <dbReference type="CAZy" id="GH103">
        <property type="family name" value="Glycoside Hydrolase Family 103"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF07618">
        <property type="protein sequence ID" value="ABF07618"/>
        <property type="gene ID" value="Rmet_0732"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0732"/>
    <dbReference type="eggNOG" id="COG2951">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035402_1_1_4"/>
    <dbReference type="OMA" id="GHTNLWD"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016798">
        <property type="term" value="F:hydrolase activity, acting on glycosyl bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008152">
        <property type="term" value="P:metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023346">
        <property type="entry name" value="Lysozyme-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011757">
        <property type="entry name" value="Lytic_transglycosylase_MltB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043426">
        <property type="entry name" value="MltB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031304">
        <property type="entry name" value="SLT_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30163">
        <property type="entry name" value="PTHR30163"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30163:SF0">
        <property type="entry name" value="PTHR30163:SF0"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13406">
        <property type="entry name" value="SLT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53955">
        <property type="entry name" value="SSF53955"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02282">
        <property type="entry name" value="MltB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SLT_2" evidence="2">
        <location>
            <begin position="94"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="191"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRSR" id="PIRSR611757-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF07618.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="408" mass="45534" checksum="A1C0FF30B1C9D6F8" modified="2006-05-30" version="1">MTCPALLRLLAVVYGAPKYALDEASRTSHPLYSVRLAKDNMTDHQRTLRRPLLGAALTAAALGLCGISPRLLAAGKRRVSVREEEIEPGRYQNHPQTRAFIDEMVANNGFQRAELEGWFSQAVYSTTVARLIMPPTTPGKKSWRAYRSRFIEPIRINAGVRFWQQNRETLRRAEAEFGVPASVIVGIIGVETIYGRDMGNFRVLDSLSTLAFDYPDTPNREARTKLFRNQLADYLLWCRDTKTDVFSVLGSYAGAIGIPQFMPTSLREYAVDYDGDGHIDLRNSPTDAIGSVGRFLQLHGWEQGRPVVWQIANDDGSRGVAAAAADGEPWPTRTLNQLTRAGLRVDEPIDVTREGETGVLIADLPTPDQPTEYVIGLRNFYVLTRYNRSFFYALTVYQLGEAVKAAMA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LQJ6</accession>
    <name>Q1LQJ6_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Replication-associated recombination protein A</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">ycaJ</name>
        <name evidence="5" type="ordered locus">Rmet_0694</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">DNA-dependent ATPase that plays important roles in cellular responses to stalled DNA replication processes.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the AAA ATPase family. RarA/MGS1/WRNIP1 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07580.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0694"/>
    <dbReference type="EnsemblBacteria" id="ABF07580">
        <property type="protein sequence ID" value="ABF07580"/>
        <property type="gene ID" value="Rmet_0694"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0694"/>
    <dbReference type="eggNOG" id="COG2256">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017985_0_3_4"/>
    <dbReference type="OMA" id="RIILSQC"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032423">
        <property type="entry name" value="AAA_assoc_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008921">
        <property type="entry name" value="DNA_pol3_clamp-load_cplx_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021886">
        <property type="entry name" value="MgsA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16193">
        <property type="entry name" value="AAA_assoc_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12002">
        <property type="entry name" value="MgsA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48019">
        <property type="entry name" value="SSF48019"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA" evidence="4">
        <location>
            <begin position="50"/>
            <end position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002393"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008959"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABF07580.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="445" mass="49222" checksum="D6B841FAEB9C7B11" modified="2006-05-30" version="1">MADSLFSDPPDRSRMPLAERLRPRNIDEVIGQQHLLGPGKPLRVAFESGEPHSMILWGPPGVGKTTLARLMANAFDAEFIALSAVLSGVKDIREAVERAEQFRAHGRRTLVFVDEVHRFNKSQQDAFLPHVESGLFTFIGATTENPSFEVNGALLSRAAVYVLKSLNEDELKQLVLRASEELGGIRWDDEAMGLIVASADGDGRKLLNNIEIVARAARNAGVDAVDTALLGSALSENLRRFDKGGDAFYDQISALHKSVRGSDPDGALYWFCRMLDGGADPRYLARRIVRMAWEDIGLADPRAARITLDAAETYERLGSPEGELALAQALLYLAVAPKSNAGYNAYNAARAFVAKDKSRPVPVHLRNAPTKLMKELGYGHAYRYAHDEPEAYAAGEHYLPDDLRSQDWYQPTPRGLEGKIGDKLRHLRDLDDAWHREQRGKSGKD</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2007-09-11" modified="2022-05-25" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LQM3</accession>
    <name>GSA_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Glutamate-1-semialdehyde 2,1-aminomutase</fullName>
            <shortName evidence="1">GSA</shortName>
            <ecNumber evidence="1">5.4.3.8</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Glutamate-1-semialdehyde aminotransferase</fullName>
            <shortName evidence="1">GSA-AT</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">hemL</name>
        <name type="ordered locus">Rmet_0667</name>
    </gene>
    <organism>
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(S)-4-amino-5-oxopentanoate = 5-aminolevulinate</text>
            <dbReference type="Rhea" id="RHEA:14265"/>
            <dbReference type="ChEBI" id="CHEBI:57501"/>
            <dbReference type="ChEBI" id="CHEBI:356416"/>
            <dbReference type="EC" id="5.4.3.8"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="1">Porphyrin-containing compound metabolism; protoporphyrin-IX biosynthesis; 5-aminolevulinate from L-glutamyl-tRNA(Glu): step 2/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family. HemL subfamily.</text>
    </comment>
    <dbReference type="EC" id="5.4.3.8" evidence="1"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07553.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515524.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q1LQM3"/>
    <dbReference type="SMR" id="Q1LQM3"/>
    <dbReference type="STRING" id="266264.Rmet_0667"/>
    <dbReference type="EnsemblBacteria" id="ABF07553">
        <property type="protein sequence ID" value="ABF07553"/>
        <property type="gene ID" value="Rmet_0667"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0667"/>
    <dbReference type="eggNOG" id="COG0001">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016922_1_5_4"/>
    <dbReference type="OMA" id="WGPLIFG"/>
    <dbReference type="OrthoDB" id="493793at2"/>
    <dbReference type="UniPathway" id="UPA00251">
        <property type="reaction ID" value="UER00317"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042286">
        <property type="term" value="F:glutamate-1-semialdehyde 2,1-aminomutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008483">
        <property type="term" value="F:transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006782">
        <property type="term" value="P:protoporphyrinogen IX biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00610">
        <property type="entry name" value="OAT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00375">
        <property type="entry name" value="HemL_aminotrans_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004639">
        <property type="entry name" value="4pyrrol_synth_GluAld_NH2Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005814">
        <property type="entry name" value="Aminotrans_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00202">
        <property type="entry name" value="Aminotran_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="SSF53383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00713">
        <property type="entry name" value="hemL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00600">
        <property type="entry name" value="AA_TRANSFER_CLASS_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0413">Isomerase</keyword>
    <keyword id="KW-0627">Porphyrin biosynthesis</keyword>
    <keyword id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000300941" description="Glutamate-1-semialdehyde 2,1-aminomutase">
        <location>
            <begin position="1"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="1">
        <location>
            <position position="270"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00375"/>
        </source>
    </evidence>
    <sequence length="430" mass="45535" checksum="05F5EBF8282986E0" modified="2006-05-30" version="1">MSRNQQLFDRAQQTIPGGVNSPVRAFRSVGGTPRFITRAEGAYMWDADGQRYIDYIGSWGPMIVGHAHPDVVRAVQETATQSFSFGAPTEAEIDMAEEICKLVPSIEQVRLVSSGTEATMSALRLARGFTGRDLIIKFEGCYHGHADSLLVKAGSGLLTFADTTQNAPSSAGVPADVTRHTMVLEYNNVTQLEEAFARHKGEIAAVIVEPVAGNMNLVRASEAFLTAMRNLCSEHGSVLIFDEVMTGFRVALGGAQAHYGIKPDMTCLGKVIGGGMPAAAFGGRRDIMAKLAPLGGVYQAGTLSGNPLAVAAGLTTLRLIQAPGFYDRLATQTRKLADGLTKAAREAGVPFCADAIGGMFGIYFTNEVPGSFAEVTKADVGRFNRFFHAMLAEGVYLAPSAFEAGFVSAKHDDAIIEATVAAAAKAFKAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="62" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LQN2</accession>
    <name>Q1LQN2_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Putative Bactoprenol-linked glucose translocase (Flippase) GtrA</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">gtrA</name>
        <name evidence="8" type="ordered locus">Rmet_0658</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the GtrA family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07544.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515515.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0658"/>
    <dbReference type="EnsemblBacteria" id="ABF07544">
        <property type="protein sequence ID" value="ABF07544"/>
        <property type="gene ID" value="Rmet_0658"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0658"/>
    <dbReference type="eggNOG" id="COG2246">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_083873_6_5_4"/>
    <dbReference type="OMA" id="GISWTAQ"/>
    <dbReference type="OrthoDB" id="1813832at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000271">
        <property type="term" value="P:polysaccharide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007267">
        <property type="entry name" value="GtrA"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04138">
        <property type="entry name" value="GtrA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="36"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="63"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="95"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="121"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="domain" description="GtrA" evidence="7">
        <location>
            <begin position="35"/>
            <end position="147"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009399"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF04138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ABF07544.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="148" mass="15754" checksum="96FF66997E60F5E7" modified="2006-05-30" version="1">MLQVQSRSRDVPGEPAGHAIWHCLPARDTLVRLFRFGVSGVIATGIHVAVATPLIYLLHASQVGANGVAFVVANIASYLLNTLWSFGATPTRDSYVRFLIVSLLGLGLTLGISAGAQALGLGYWAGLAAILSVVPAVTFVLHRTWTYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="97" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LR00</accession>
    <name>Q1LR00_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Putative regulatory protein, TetR family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Rmet_0540</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07426.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0540"/>
    <dbReference type="EnsemblBacteria" id="ABF07426">
        <property type="protein sequence ID" value="ABF07426"/>
        <property type="gene ID" value="Rmet_0540"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0540"/>
    <dbReference type="eggNOG" id="COG1309">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_105787_0_0_4"/>
    <dbReference type="OMA" id="LRHYSAR"/>
    <dbReference type="OrthoDB" id="1309950at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001647">
        <property type="entry name" value="HTH_TetR"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00440">
        <property type="entry name" value="TetR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50977">
        <property type="entry name" value="HTH_TETR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH tetR-type" evidence="3">
        <location>
            <begin position="37"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="H-T-H motif" evidence="1">
        <location>
            <begin position="61"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF07426.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="247" mass="27376" checksum="41E0F64AB2E5DC9C" modified="2006-05-30" version="1">MPTPTKPTTRSATASRARSAKRGADEVVRPPLQSRSEKSLEKMVAACRALAEERGNLDDISLNETVARAGTSIGAFYGRFKDKEAFLGYVLEVALREAEDVMQRSIDEEPVWQTGPAQAIVERIIGSYVSQFRLNRGLFRGFLRHYSARDARDNPMRDANRRIYGLVVPSLAAQLGDRPKDTAEFEVRAALQFMVGTLANILLNDPGPLHLDDDALALHLDRMMVRYLGLPEAVAPVASATPRRSKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LRC0</accession>
    <name>Q1LRC0_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Phosphoglycerate mutase 2 protein</fullName>
            <ecNumber evidence="2">5.4.2.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">gpmB</name>
        <name evidence="2" type="ordered locus">Rmet_0420</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EC" id="5.4.2.-" evidence="2"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07306.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515295.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0420"/>
    <dbReference type="EnsemblBacteria" id="ABF07306">
        <property type="protein sequence ID" value="ABF07306"/>
        <property type="gene ID" value="Rmet_0420"/>
    </dbReference>
    <dbReference type="GeneID" id="60824022"/>
    <dbReference type="KEGG" id="rme:Rmet_0420"/>
    <dbReference type="eggNOG" id="COG0406">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033323_9_5_4"/>
    <dbReference type="OMA" id="TEWNVAR"/>
    <dbReference type="OrthoDB" id="1122642at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016853">
        <property type="term" value="F:isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006094">
        <property type="term" value="P:gluconeogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd07067">
        <property type="entry name" value="HP_PGM_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1240">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013078">
        <property type="entry name" value="His_Pase_superF_clade-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029033">
        <property type="entry name" value="His_PPase_superfam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001345">
        <property type="entry name" value="PG/BPGM_mutase_AS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00300">
        <property type="entry name" value="His_Phos_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00855">
        <property type="entry name" value="PGAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53254">
        <property type="entry name" value="SSF53254"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00175">
        <property type="entry name" value="PG_MUTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0312">Gluconeogenesis</keyword>
    <keyword evidence="2" id="KW-0413">Isomerase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF07306.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="224" mass="25107" checksum="9BFA4B78A7990FE3" modified="2006-05-30" version="1">MSQSTGPQSLAYTHLIVIRHGETAWNRERRLQGQLDIPLNDTGRAQARALAEALAGEPIDAVYSSDLGRAMQTAAPLAETLGLQVRPERRLRERCYGDLEGMTYAEVAEKRPEDFARWQARVPDYAPPEGESLREFHDRAVEVALYLSRRHPGERIALVAHGGVLDCLYREAAGMTLEAPREHELLNASVNRLRCDTAHLTLLQWADVSHLEALALDEVDRRVP</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="98" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LRJ1</accession>
    <name>Q1LRJ1_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="10">ABC-type multidrug transporter, ATPase component</fullName>
            <ecNumber evidence="10">3.6.3.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">Rmet_0349</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EC" id="3.6.3.-" evidence="10"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07235.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515228.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0349"/>
    <dbReference type="EnsemblBacteria" id="ABF07235">
        <property type="protein sequence ID" value="ABF07235"/>
        <property type="gene ID" value="Rmet_0349"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0349"/>
    <dbReference type="eggNOG" id="COG1131">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_2_4"/>
    <dbReference type="OMA" id="TQRFSYW"/>
    <dbReference type="OrthoDB" id="1926714at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="1" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-1278">Translocase</keyword>
    <feature type="domain" description="ABC transporter" evidence="9">
        <location>
            <begin position="7"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="309"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="313"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="39"/>
            <end position="46"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ABF07235.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="328" mass="35774" checksum="23F6A2D7DAF55775" modified="2006-05-30" version="1">MNGELAIDVHGLNKRFGDKHVVNDLTMQVARGEIFGFLGPNGSGKTTSIRMMCGLLTPDSGAGTCLGFDIIKESAEIKRNVGYMTQRFSYWEDLSIRENLDFVARVYAMPNRREVVDRALEDLGLASRAEQLAGSLSGGWKQRLALAACLLHEPQLLLLDEPTAGVDPKARRDFWEQLHQLAARGISVLVSTHYMDEAERCHKLAYIAYGKLLAQGTSAEVVASQNLTTWSVSGDELAELSTQLQGKPGVDQTVAFGTALHVTGKDGTALEQTLRQYAGRDHRIEPVETSLEDVFIHMMGDAQDNMAVPAKEATKESAERASGPKGDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="106" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LRQ1</accession>
    <name>Q1LRQ1_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Ribose-phosphate pyrophosphokinase</fullName>
            <shortName evidence="6">RPPK</shortName>
            <ecNumber evidence="6">2.7.6.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">5-phospho-D-ribosyl alpha-1-diphosphate</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">Phosphoribosyl diphosphate synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">Phosphoribosyl pyrophosphate synthase</fullName>
            <shortName evidence="6">P-Rib-PP synthase</shortName>
            <shortName evidence="6">PRPP synthase</shortName>
            <shortName evidence="6">PRPPase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6 8" type="primary">prs</name>
        <name evidence="8" type="ordered locus">Rmet_0289</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Involved in the biosynthesis of the central metabolite phospho-alpha-D-ribosyl-1-pyrophosphate (PRPP) via the transfer of pyrophosphoryl group from ATP to 1-hydroxyl of ribose-5-phosphate (Rib-5-P).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>ATP + D-ribose 5-phosphate = 5-phospho-alpha-D-ribose 1-diphosphate + AMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:15609"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <dbReference type="ChEBI" id="CHEBI:78346"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="2.7.6.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="6">Binds 2 Mg(2+) ions per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="6">Metabolic intermediate biosynthesis; 5-phospho-alpha-D-ribose 1-diphosphate biosynthesis; 5-phospho-alpha-D-ribose 1-diphosphate from D-ribose 5-phosphate (route I): step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Homohexamer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the ribose-phosphate pyrophosphokinase family. Class I subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.6.1" evidence="6"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07175.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008650936.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0289"/>
    <dbReference type="EnsemblBacteria" id="ABF07175">
        <property type="protein sequence ID" value="ABF07175"/>
        <property type="gene ID" value="Rmet_0289"/>
    </dbReference>
    <dbReference type="GeneID" id="60823014"/>
    <dbReference type="KEGG" id="rme:Rmet_0289"/>
    <dbReference type="eggNOG" id="COG0462">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033546_2_0_4"/>
    <dbReference type="OMA" id="FGWARQD"/>
    <dbReference type="OrthoDB" id="1011035at2"/>
    <dbReference type="UniPathway" id="UPA00087">
        <property type="reaction ID" value="UER00172"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004749">
        <property type="term" value="F:ribose phosphate diphosphokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006015">
        <property type="term" value="P:5-phosphoribose 1-diphosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009165">
        <property type="term" value="P:nucleotide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009156">
        <property type="term" value="P:ribonucleoside monophosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd06223">
        <property type="entry name" value="PRTases_typeI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2020">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00583_B">
        <property type="entry name" value="RibP_PPkinase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000842">
        <property type="entry name" value="PRib_PP_synth_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029099">
        <property type="entry name" value="Pribosyltran_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000836">
        <property type="entry name" value="PRibTrfase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029057">
        <property type="entry name" value="PRTase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005946">
        <property type="entry name" value="Rib-P_diPkinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037515">
        <property type="entry name" value="Rib-P_diPkinase_bac"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10210">
        <property type="entry name" value="PTHR10210"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14572">
        <property type="entry name" value="Pribosyl_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13793">
        <property type="entry name" value="Pribosyltran_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53271">
        <property type="entry name" value="SSF53271"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01251">
        <property type="entry name" value="ribP_PPkin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00114">
        <property type="entry name" value="PRPP_SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 6 8" id="KW-0418">Kinase</keyword>
    <keyword evidence="6" id="KW-0460">Magnesium</keyword>
    <keyword evidence="2 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3 6" id="KW-0545">Nucleotide biosynthesis</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 6 8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Pribosyltran_N" evidence="7">
        <location>
            <begin position="6"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="region of interest" description="Ribose-5-phosphate binding" evidence="6">
        <location>
            <begin position="225"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="active site" evidence="6">
        <location>
            <position position="195"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="39"/>
            <end position="41"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="98"/>
            <end position="99"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="133"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="172"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="197"/>
        </location>
        <ligand>
            <name>D-ribose 5-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:78346"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="221"/>
        </location>
        <ligand>
            <name>D-ribose 5-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:78346"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF13793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ABF07175.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="317" mass="34116" checksum="DC1A0247F69223D4" modified="2006-05-30" version="1">MSVEGLMVFTGNANPKLAEAVVQHLGIPLGKALVGRFSDGEVQVEIQENVRGKHVIVLQSTCAPTNDNLMELMVMVDALKRASARSITAAMPYFGYARQDRRPRSARVAISAKIVANMLEVAGVERVLTMDLHADQIQGFFDIPVDNIYASPVLLGDLREKNYGNLLVVSPDVGGVVRARALAKQLDCDLAIIDKRRPKANVSEVMNIIGEVAGRNCVIMDDMIDTGGTLCKAAQVLKERGAQKVFAYCTHPVLSGGAVARIAASALDEVVVCDTIPLSDEARQTGKIRQVTTAPLLAETFTRIVRGDSIMSLFADA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LS67</accession>
    <name>Q1LS67_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Outer membrane receptor, TonB dependent</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">Rmet_0123</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cell outer membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the TonB-dependent receptor family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07009.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515041.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0123"/>
    <dbReference type="EnsemblBacteria" id="ABF07009">
        <property type="protein sequence ID" value="ABF07009"/>
        <property type="gene ID" value="Rmet_0123"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0123"/>
    <dbReference type="eggNOG" id="COG4206">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008287_10_1_4"/>
    <dbReference type="OMA" id="YTWADGF"/>
    <dbReference type="OrthoDB" id="439303at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009279">
        <property type="term" value="C:cell outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.130.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.170.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039426">
        <property type="entry name" value="BtuB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012910">
        <property type="entry name" value="Plug_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037066">
        <property type="entry name" value="Plug_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000531">
        <property type="entry name" value="TonB-dep_rcpt_b-brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036942">
        <property type="entry name" value="TonB_rcpt_b-brl_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30069">
        <property type="entry name" value="PTHR30069"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07715">
        <property type="entry name" value="Plug"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00593">
        <property type="entry name" value="TonB_dep_Rec"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0998">Cell outer membrane</keyword>
    <keyword evidence="3 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 11" id="KW-0675">Receptor</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <keyword evidence="2 6" id="KW-0798">TonB box</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193484" evidence="8">
        <location>
            <begin position="35"/>
            <end position="719"/>
        </location>
    </feature>
    <feature type="domain" description="Plug" evidence="10">
        <location>
            <begin position="71"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="domain" description="TonB_dep_Rec" evidence="9">
        <location>
            <begin position="264"/>
            <end position="716"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="250"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="259"/>
            <end position="273"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009810"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003357"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00593"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF07715"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ABF07009.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="719" mass="76094" checksum="2AF4AEE8E21DC9D4" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="1998-12-15" modified="2022-05-25" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>Q44009</accession>
    <accession>O07951</accession>
    <name>CZCI_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName>Cobalt-zinc-cadmium resistance protein CzcI</fullName>
        </recommendedName>
        <alternativeName>
            <fullName>Cation efflux system protein CzcI</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">czcI</name>
        <name type="ordered locus">Rmet_5983.1</name>
    </gene>
    <organism>
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid">
        <name>pMOL30</name>
    </geneLocation>
    <reference key="1">
        <citation type="journal article" date="1992" name="J. Bacteriol." volume="174" first="8102" last="8110">
            <title>CzcR and CzcD, gene products affecting regulation of resistance to cobalt, zinc, and cadmium (czc system) in Alcaligenes eutrophus.</title>
            <authorList>
                <person name="Nies D.H."/>
            </authorList>
            <dbReference type="PubMed" id="1459958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC DNA]</scope>
    </reference>
    <reference key="2">
        <citation type="journal article" date="1995" name="J. Ind. Microbiol." volume="14" first="142" last="153">
            <title>The czc operon of Alcaligenes eutrophus CH34: from resistance mechanism to the removal of heavy metals.</title>
            <authorList>
                <person name="Diels L."/>
                <person name="Dong Q."/>
                <person name="van der Lelie D."/>
                <person name="Baeyens W."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="7766206"/>
            <dbReference type="DOI" id="10.1007/BF01569896"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC DNA]</scope>
        <scope>IDENTIFICATION</scope>
        <scope>INDUCTION</scope>
        <scope>DISCUSSION OF FUNCTION</scope>
    </reference>
    <reference key="3">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text>Component of the czc cation-efflux system that confers resistance to cobalt, zinc and cadmium. May have a regulatory function.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Periplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="induction">
        <text evidence="3">By zinc, cobalt and cadmium (zinc being the best and cadmium being the worst inducer).</text>
    </comment>
    <dbReference type="EMBL" id="X71400">
        <property type="protein sequence ID" value="CAA50524.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="X98451">
        <property type="protein sequence ID" value="CAA67081.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP000354">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011229345.1">
        <property type="nucleotide sequence ID" value="NC_007971.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_145592.1">
        <property type="nucleotide sequence ID" value="NC_006466.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q44009"/>
    <dbReference type="GeneID" id="60820447"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid pMOL30"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042597">
        <property type="term" value="C:periplasmic space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046686">
        <property type="term" value="P:response to cadmium ion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword id="KW-0105">Cadmium resistance</keyword>
    <keyword id="KW-0170">Cobalt</keyword>
    <keyword id="KW-0574">Periplasm</keyword>
    <keyword id="KW-0614">Plasmid</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0732">Signal</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000021057" description="Cobalt-zinc-cadmium resistance protein CzcI">
        <location>
            <begin position="21"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="93"/>
            <end position="115"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="3">
        <source>
            <dbReference type="PubMed" id="7766206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="4"/>
    <sequence length="115" mass="13266" checksum="9B8AC92FB8719834" modified="1996-11-01" version="1" precursor="true">MRRFVLIFVLLILPFQFSWAAAARYCQHEKATATWHLGHHEHRHQQPEGKTDAEKKPFVDTDCGVCHLVSLPFVYGQTQDVLIANRVEVTDTQHSSEFSSLNARAPDRPQWQRLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-04-26" modified="2021-09-29" version="69" xmlns="http://uniprot.org/uniprot">
    <accession>Q58AC9</accession>
    <name>Q58AC9_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Antitoxin of a toxin/antitoxin system</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_6100</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2 3">
        <name>pMOL30</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid pMOL30</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000354">
        <property type="protein sequence ID" value="ABF12959.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008652555.1">
        <property type="nucleotide sequence ID" value="NC_007971.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_145689.1">
        <property type="nucleotide sequence ID" value="NC_006466.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF12959">
        <property type="protein sequence ID" value="ABF12959"/>
        <property type="gene ID" value="Rmet_6100"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6100"/>
    <dbReference type="HOGENOM" id="CLU_179376_1_1_4"/>
    <dbReference type="OMA" id="STICCNI"/>
    <dbReference type="OrthoDB" id="2064132at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid pMOL30"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019239">
        <property type="entry name" value="VapB_antitoxin"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09957">
        <property type="entry name" value="VapB_antitoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="44"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="57"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF12959.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="78" mass="8446" checksum="EB5DADADF0C26ECB" modified="2005-04-26" version="1">MRTTITLDDELLAKARAYTGLEEKTALVREALKALIQREAAKRLANLGGSQPGIEGAPRRRSDDDENRIGEPPDGGSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-04-26" modified="2022-08-03" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>Q58AG2</accession>
    <name>Q58AG2_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">NccA, three components proton antiporter cation efflux system, cation efflux pump</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">nccA</name>
        <name evidence="4" type="ordered locus">Rmet_6145</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4 5">
        <name>pMOL30</name>
    </geneLocation>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="5">Plasmid pMOL30</plasmid>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the resistance-nodulation-cell division (RND) (TC 2.A.6) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000354">
        <property type="protein sequence ID" value="ABF13004.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008642698.1">
        <property type="nucleotide sequence ID" value="NC_007971.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_145656.1">
        <property type="nucleotide sequence ID" value="NC_006466.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF13004">
        <property type="protein sequence ID" value="ABF13004"/>
        <property type="gene ID" value="Rmet_6145"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6145"/>
    <dbReference type="HOGENOM" id="CLU_002755_1_2_4"/>
    <dbReference type="OMA" id="INVFYDR"/>
    <dbReference type="OrthoDB" id="91817at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid pMOL30"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008324">
        <property type="term" value="F:cation transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2090.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027463">
        <property type="entry name" value="AcrB_DN_DC_subdom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001036">
        <property type="entry name" value="Acrflvin-R"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004763">
        <property type="entry name" value="CzcA/CusA/SilA/NccA/HelA/CnrA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32063">
        <property type="entry name" value="PTHR32063"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00873">
        <property type="entry name" value="ACR_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00702">
        <property type="entry name" value="ACRIFLAVINRP"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82714">
        <property type="entry name" value="SSF82714"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00914">
        <property type="entry name" value="2A0601"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0614">Plasmid</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="419"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="477"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="508"/>
            <end position="531"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="561"/>
            <end position="582"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="904"/>
            <end position="923"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="930"/>
            <end position="949"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="955"/>
            <end position="977"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="1005"/>
            <end position="1027"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="1033"/>
            <end position="1060"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF13004.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="1076" mass="115692" checksum="FD8B7C44CFC57EB9" modified="2005-04-26" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2005-04-26" modified="2021-06-02" version="68" xmlns="http://uniprot.org/uniprot">
    <accession>Q58AQ4</accession>
    <name>Q58AQ4_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_6016</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="1 2">
        <name>pMOL30</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid pMOL30</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000354">
        <property type="protein sequence ID" value="ABF12875.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011229315.1">
        <property type="nucleotide sequence ID" value="NC_007971.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_145561.1">
        <property type="nucleotide sequence ID" value="NC_006466.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF12875">
        <property type="protein sequence ID" value="ABF12875"/>
        <property type="gene ID" value="Rmet_6016"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6016"/>
    <dbReference type="HOGENOM" id="CLU_2104455_0_0_4"/>
    <dbReference type="OMA" id="WETYARE"/>
    <dbReference type="OrthoDB" id="2167657at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid pMOL30"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF12875.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="121" mass="13480" checksum="98E0C2019B5B04A7" modified="2005-04-26" version="1">MAQKIVRWRSNYRGEARFDVVAGPLAGETVAVRFLKPDYYEKDFRDNAGGVFLPDWGVYTREVMSSVGIVRSTLHPAGLPTDIVEAVNADFDDLRASSDPGIFFGPFLNGRYDPQVGWVAH</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-04-26" modified="2021-06-02" version="70" xmlns="http://uniprot.org/uniprot">
    <accession>Q58AQ9</accession>
    <name>Q58AQ9_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_6022</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="1 2">
        <name>pMOL30</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid pMOL30</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000354">
        <property type="protein sequence ID" value="ABF12881.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011229310.1">
        <property type="nucleotide sequence ID" value="NC_007971.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_145556.1">
        <property type="nucleotide sequence ID" value="NC_006466.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF12881">
        <property type="protein sequence ID" value="ABF12881"/>
        <property type="gene ID" value="Rmet_6022"/>
    </dbReference>
    <dbReference type="GeneID" id="60824593"/>
    <dbReference type="KEGG" id="rme:Rmet_6022"/>
    <dbReference type="HOGENOM" id="CLU_2059455_0_0_4"/>
    <dbReference type="OMA" id="VCKDSAV"/>
    <dbReference type="OrthoDB" id="972781at1224"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid pMOL30"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF12881.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="119" mass="12696" checksum="6DD83C7BA5A17DD2" modified="2005-04-26" version="1">MKISSEIKDQAKYLESYVVLDTVTAAGKFAVGLGIGNVVREGLYLTPVGQAAYERLKELGKGSPQLIDVASKFKAKELVRQAFLKADAGGSIFFVCREGIYDTVYDLLNVIGSSTAAAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-01-04" modified="2021-09-29" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>Q5NUW1</accession>
    <name>Q5NUW1_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Outer membrane lipoprotein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">slyB</name>
        <name evidence="2" type="ordered locus">Rmet_6233</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2 3">
        <name>pMOL28</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid pMOL28</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000355">
        <property type="protein sequence ID" value="ABF13092.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011239949.1">
        <property type="nucleotide sequence ID" value="NC_007972.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_161688.1">
        <property type="nucleotide sequence ID" value="NC_006525.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF13092">
        <property type="protein sequence ID" value="ABF13092"/>
        <property type="gene ID" value="Rmet_6233"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6233"/>
    <dbReference type="HOGENOM" id="CLU_144017_0_0_4"/>
    <dbReference type="OMA" id="YQRYDVQ"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid pMOL28"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5013532132" evidence="1">
        <location>
            <begin position="20"/>
            <end position="157"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF13092.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="157" mass="16285" checksum="8DA0668C33E8C1E8" modified="2005-01-04" version="1">MFKRIASLFLASIIFTGCAAQQTSPNSYRSSEALRTGSAEVVTVIRLRQVTIIDSDGYSSANSGIPGVVGALVGGLLGARVIGGGNGRYVAGAMSGTVSAVLAQGVASQLNRRAGVEVVVRKENGSQIVLTQDADQQFTTGEQLYLVSSGNGYRLTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-01-04" modified="2021-06-02" version="61" xmlns="http://uniprot.org/uniprot">
    <accession>Q5NV28</accession>
    <name>Q5NV28_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_6272</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="1 2">
        <name>pMOL28</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid pMOL28</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000355">
        <property type="protein sequence ID" value="ABF13131.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011239922.1">
        <property type="nucleotide sequence ID" value="NC_007972.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_161662.1">
        <property type="nucleotide sequence ID" value="NC_006525.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF13131">
        <property type="protein sequence ID" value="ABF13131"/>
        <property type="gene ID" value="Rmet_6272"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6272"/>
    <dbReference type="HOGENOM" id="CLU_731313_0_0_4"/>
    <dbReference type="OrthoDB" id="2034176at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid pMOL28"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF13131.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="378" mass="43331" checksum="7E1A3609EC4AE47B" modified="2005-01-04" version="1">MPKAIAFFPWVAINGARSFGALRLIPYELGKAPGDLPYATQADIEAVLGAYAEHPRHPIRAATLVEFGDWQTGMDIEDRAQWLWALQKLVAFAALANRRLFTVAYTNTDTYKLVIQRYRAGEANRFGFHTRRRDGKGLHSWGTDEFAFHRPHHVSRSWFSIDEHLLCALMPDMPPHWRSAIEDYCAANTDSRDMPDHTEAVLLYCAFEWLFGFSNNCKKFIDALDRTFPYHADMQGNGPLADDWKGKYQHVTRPLTAWASEFCQVRGQSAHGLSREVRRFVWPVHTHLAFASMLFPLAFKKTLADAGRFEISEGDLQRMRQIDAYVLHDPFAFDFTMETEDNKHPWCELDTEHTLRAMIEQSMREVMVGKSPGADTEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-03-23" modified="2021-06-02" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>D3DXV5</accession>
    <name>D3DXV5_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_6517</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ADC45125.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_6517"/>
    <dbReference type="EnsemblBacteria" id="ADC45125">
        <property type="protein sequence ID" value="ADC45125"/>
        <property type="gene ID" value="Rmet_6517"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6517"/>
    <dbReference type="HOGENOM" id="CLU_3398083_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="11"/>
            <end position="31"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADC45125.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="31" mass="3405" checksum="7759FE15300612E5" modified="2010-03-23" version="1">MSGRKGCDVETTETLTPEKGRARKGVRDVQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-03-23" modified="2020-10-07" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>D3DY47</accession>
    <name>D3DY47_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_6618</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ADC45217.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_6618"/>
    <dbReference type="EnsemblBacteria" id="ADC45217">
        <property type="protein sequence ID" value="ADC45217"/>
        <property type="gene ID" value="Rmet_6618"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6618"/>
    <dbReference type="HOGENOM" id="CLU_2993483_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADC45217.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="57" mass="6847" checksum="DB477EBB14347973" modified="2010-03-23" version="1">MQDAARRCRFYCPSFNNWRFLIPKHENLAMLFQTYVPHYADSYLAQMGGMDETFHIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-03-23" modified="2020-10-07" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>D3DYB1</accession>
    <name>D3DYB1_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_6698</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ADC45281.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_6698"/>
    <dbReference type="EnsemblBacteria" id="ADC45281">
        <property type="protein sequence ID" value="ADC45281"/>
        <property type="gene ID" value="Rmet_6698"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6698"/>
    <dbReference type="HOGENOM" id="CLU_2737167_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADC45281.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="71" mass="7645" checksum="0682AC8AC473D070" modified="2010-03-23" version="1">MPRGRSAHATDPALPQGARRAALPPTLAPQLAKLADHPPAANANWRYEVKFESASACAWEDRGAEPCHDKT</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1998-12-15" modified="2022-08-03" version="124" xmlns="http://uniprot.org/uniprot">
    <accession>O30330</accession>
    <accession>Q1LJ27</accession>
    <name>FUR_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName>Ferric uptake regulation protein</fullName>
            <shortName>Ferric uptake regulator</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">fur</name>
        <name type="synonym">aleO</name>
        <name type="ordered locus">Rmet_2976</name>
    </gene>
    <organism>
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="1997-08" db="EMBL/GenBank/DDBJ databases">
            <title>Identification and regulation of the Alcaligenes eutrophus CH34 fur gene.</title>
            <authorList>
                <person name="Gilis A."/>
                <person name="Borremans B."/>
                <person name="Cornelis P."/>
                <person name="Mergeay M."/>
                <person name="van der Lelie D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC DNA]</scope>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text>Acts as a global negative controlling element, employing Fe(2+) as a cofactor to bind the operator of the repressed genes. Regulator for biosynthesis of the siderophore alcaligin E.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Fur family.</text>
    </comment>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="component" value="Chromosome"/>
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        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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        <property type="term" value="F:metal ion binding"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="WH-like_DNA-bd_sf"/>
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    </dbReference>
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        <property type="entry name" value="PTHR33202"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01475">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0238">DNA-binding</keyword>
    <keyword id="KW-0408">Iron</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0678">Repressor</keyword>
    <keyword id="KW-0804">Transcription</keyword>
    <keyword id="KW-0805">Transcription regulation</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <feature type="chain" id="PRO_0000095540" description="Ferric uptake regulation protein">
        <location>
            <begin position="1"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="region of interest" description="DNA-binding" evidence="1">
        <location>
            <begin position="1"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="region of interest" description="Dimerization" evidence="1">
        <location>
            <begin position="84"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="32"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="80"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
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    <feature type="binding site" evidence="1">
        <location>
            <position position="86"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
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    <feature type="binding site" evidence="1">
        <location>
            <position position="88"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
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    <feature type="binding site" evidence="1">
        <location>
            <position position="89"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
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        </ligand>
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    <feature type="binding site" evidence="1">
        <location>
            <position position="92"/>
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        <ligand>
            <name>Zn(2+)</name>
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        </ligand>
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        </location>
        <ligand>
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        </ligand>
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    <feature type="binding site" evidence="1">
        <location>
            <position position="100"/>
        </location>
        <ligand>
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            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="107"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
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    <feature type="binding site" evidence="1">
        <location>
            <position position="124"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="143" mass="16432" checksum="D88B8E603901E49B" modified="1998-01-01" version="1">MPSPADLKNIGLKATVPRLKILEIFQTSEQRHLSAEDVYRILLNEHMDIGLATVYRVLTQFEQAGLLSRNNFESGKAIFELNEGKHHDHLVCLDCGRVEEFFDADIEQRQQSIARERGFALQEHALSLYGNCTKDDCPHRPRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="59" xmlns="http://uniprot.org/uniprot">
    <accession>Q1L9T1</accession>
    <name>Q1L9T1_CUPMC</name>
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        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Rmet_6236</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
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        <name>pMOL28</name>
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    <reference evidence="9" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
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                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
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                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
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        </source>
    </reference>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="1" id="KW-0645">Protease</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="DUF2016" evidence="6">
        <location>
            <begin position="4"/>
            <end position="75"/>
        </location>
    </feature>
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        <location>
            <begin position="84"/>
            <end position="177"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF09436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF14464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ABF13095.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="213" mass="23599" checksum="F0FBBD3E7EA527E0" modified="2006-05-30" version="1">MHPADMALQQSFPSVMVPRHGALPALEQVGERLLIAANGVFLEIVRPWLRVVRRLGEFQHQTAIPYGDATEVTELRCGRVPPALIGEFTDMARAAYPNETGAFVVWNARTQQFRLVPLRILAQGTGHLKYDRPRLGADDVLVVDCHSHGRYPAFFSATDDADDRHDVKFAFVIGNCNAAVPSLALRLCAKGIFENVERIPHGWYDAARAEVSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="66" xmlns="http://uniprot.org/uniprot">
    <accession>Q1L9X3</accession>
    <name>Q1L9X3_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">WD40/YVTN repeat-like-containing domain beta-propeller repeat protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">chrZ</name>
        <name evidence="1" type="ordered locus">Rmet_6194</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
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            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="1 2">
        <name>pMOL28</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid pMOL28</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000355">
        <property type="protein sequence ID" value="ABF13053.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_007972.2"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF13053">
        <property type="protein sequence ID" value="ABF13053"/>
        <property type="gene ID" value="Rmet_6194"/>
    </dbReference>
    <dbReference type="GeneID" id="60825789"/>
    <dbReference type="KEGG" id="rme:Rmet_6194"/>
    <dbReference type="HOGENOM" id="CLU_043515_2_0_4"/>
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    <dbReference type="OrthoDB" id="302683at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid pMOL28"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011048">
        <property type="entry name" value="Haem_d1_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51004">
        <property type="entry name" value="SSF51004"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF13053.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="348" mass="37668" checksum="BA913CD3C1EAF6C3" modified="2006-05-30" version="1">MFTRRTPREQQSLRYHGLSVLALLFAMLLDPAPCLSGTTTMPSRALPLQHVGDFRLPGGKTRWDYLSLDPTRSKLFIAHLGDSEIVVMDTKTKTIAATISNVGHVHGTLAIPELNRVYASATGTDEVVAIDASTFKIVARIPGGKYPDGIAYAPEVGKLYVSDEHGNTETVIDVRTNRRVATIELGGAVGNTQYDPVSKHIFVNVQGRQSLAEIDPQTDRVLDRIALPGAEGNHGLLIVPKFRLAFIVCEGIDRLLVMDLEAKRIVANFGVGGEPDVLGYDEDLGQVYVASESGVLSVFKVTATDVTKVAEGFVGANAHSLAVDPASHEIYLPLKNADHHPAMRVMRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LA75</accession>
    <name>Q1LA75_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_6092</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2 3">
        <name>pMOL30</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid pMOL30</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000354">
        <property type="protein sequence ID" value="ABF12951.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011514851.1">
        <property type="nucleotide sequence ID" value="NC_007971.2"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF12951">
        <property type="protein sequence ID" value="ABF12951"/>
        <property type="gene ID" value="Rmet_6092"/>
    </dbReference>
    <dbReference type="GeneID" id="60825028"/>
    <dbReference type="KEGG" id="rme:Rmet_6092"/>
    <dbReference type="HOGENOM" id="CLU_1110677_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid pMOL30"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="79"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="119"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="142"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="172"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="207"/>
            <end position="224"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF12951.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="250" mass="27622" checksum="0C1155730D8BF89C" modified="2006-05-30" version="1">MIRFKLPAMRIANVAILFSTYFAYAVAVSAYSYAVAAHRWAAPGKPQAERFSTEVRQYVSALQNWETQWHSLFPNTLDHLLFGIWCAALVSIAALVLLCSDQNAGTGTYRLGRKLRISLAATLAVLAFCGLSIRPYSWLDYITAASAGLVIVAVGISNCNVIGFARRRPSAFVFVLGIFLWLRLGVGWEFHQWLIDDPKAEGTPRELTASIVGLIVGSYLWGKVCRGLERILRRIRSGRSQQNVVVNAAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="100" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LAS7</accession>
    <name>Q1LAS7_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Ferrous iron transport protein B</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">feoB</name>
        <name evidence="14" type="ordered locus">Rmet_5890</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="15">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="15">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Probable transporter of a GTP-driven Fe(2+) uptake system.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="12">Cell inner membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily. FeoB GTPase (TC 9.A.8) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12749.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518098.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5890"/>
    <dbReference type="EnsemblBacteria" id="ABF12749">
        <property type="protein sequence ID" value="ABF12749"/>
        <property type="gene ID" value="Rmet_5890"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5890"/>
    <dbReference type="eggNOG" id="COG0370">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013350_3_0_4"/>
    <dbReference type="OMA" id="PLGYDWK"/>
    <dbReference type="OrthoDB" id="304844at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015093">
        <property type="term" value="F:ferrous iron transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055072">
        <property type="term" value="P:iron ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01879">
        <property type="entry name" value="FeoB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003373">
        <property type="entry name" value="Fe2_transport_prot-B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011640">
        <property type="entry name" value="Fe2_transport_prot_B_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030389">
        <property type="entry name" value="G_FEOB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011642">
        <property type="entry name" value="Gate_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07664">
        <property type="entry name" value="FeoB_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02421">
        <property type="entry name" value="FeoB_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07670">
        <property type="entry name" value="Gate"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00326">
        <property type="entry name" value="GTP1OBG"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00437">
        <property type="entry name" value="feoB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51711">
        <property type="entry name" value="G_FEOB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10 12" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="9" id="KW-0406">Ion transport</keyword>
    <keyword evidence="8 12" id="KW-0408">Iron</keyword>
    <keyword evidence="4 12" id="KW-0410">Iron transport</keyword>
    <keyword evidence="11 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-0614">Plasmid</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 12" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="222"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="277"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="362"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="398"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="462"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="561"/>
            <end position="584"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="596"/>
            <end position="616"/>
        </location>
    </feature>
    <feature type="domain" description="FeoB-type G" evidence="13">
        <location>
            <begin position="10"/>
            <end position="178"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU362098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51711"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ABF12749.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="620" mass="67341" checksum="CC46F2D0BB18F083" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-10-07" version="59" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LB37</accession>
    <name>Q1LB37_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_5780</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12639.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5780"/>
    <dbReference type="EnsemblBacteria" id="ABF12639">
        <property type="protein sequence ID" value="ABF12639"/>
        <property type="gene ID" value="Rmet_5780"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5780"/>
    <dbReference type="eggNOG" id="COG1814">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1234202_0_0_4"/>
    <dbReference type="OMA" id="LGCNLAW"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="32"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="64"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="159"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="185"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="212"/>
            <end position="233"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF12639.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="235" mass="24596" checksum="389914790370B636" modified="2006-05-30" version="1">MPGSQSESDAMEQADEGRDPALNVVDRVAEMCFGLFMALTFVGAVSSATSAASPTSGADAGHTMLVTALGCNLAWGLVDAIMFLVRTLADRGMRFKVATAAREAPTATEAVRAIRTALSPSAQLLLSDAEIDMLRQRMANAVELPERAKLNARDLLGSIRIFFIVVLSTFPVALPFVLLSDVPTALIVSRILTLIMLFVGGMALGRHAGFNGWATGLGMTALGVVLTMAVIALGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LB45</accession>
    <name>Q1LB45_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transcriptional regulator, MarR family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_5772</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12631.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008652586.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF12631">
        <property type="protein sequence ID" value="ABF12631"/>
        <property type="gene ID" value="Rmet_5772"/>
    </dbReference>
    <dbReference type="GeneID" id="60823517"/>
    <dbReference type="KEGG" id="rme:Rmet_5772"/>
    <dbReference type="eggNOG" id="COG1846">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_083287_18_4_4"/>
    <dbReference type="OMA" id="PDENDGR"/>
    <dbReference type="OrthoDB" id="1744037at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000835">
        <property type="entry name" value="HTH_MarR-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12802">
        <property type="entry name" value="MarR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00598">
        <property type="entry name" value="HTHMARR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00347">
        <property type="entry name" value="HTH_MARR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50995">
        <property type="entry name" value="HTH_MARR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH marR-type" evidence="1">
        <location>
            <begin position="2"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF12631.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="142" mass="15555" checksum="E9C45BDFD061A035" modified="2006-05-30" version="1">MNPRLVYLLNVGQRRLHRWTQARTAAGGVTASQAGMLFFLGKNDGALMSEAGAALDLGAPGVSGLVDRMERAGLVERGLDEHDRRAFRIWLTDAGRTALKRSRSNLTELNARLTEGFTAAEIDVVARWLTSLQDKFPASDDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LBI2</accession>
    <name>Q1LBI2_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Penicillin amidase (Peptidase S45)</fullName>
            <ecNumber evidence="7">3.5.1.11</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Rmet_5635</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="8">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="8">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
        <text evidence="5">Binds 1 Ca(2+) ion per dimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase S45 family.</text>
    </comment>
    <dbReference type="EC" id="3.5.1.11" evidence="7"/>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12494.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011520038.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5635"/>
    <dbReference type="MEROPS" id="S45.003"/>
    <dbReference type="EnsemblBacteria" id="ABF12494">
        <property type="protein sequence ID" value="ABF12494"/>
        <property type="gene ID" value="Rmet_5635"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5635"/>
    <dbReference type="eggNOG" id="COG2366">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011790_0_1_4"/>
    <dbReference type="OMA" id="MDELPWA"/>
    <dbReference type="OrthoDB" id="186419at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008953">
        <property type="term" value="F:penicillin amidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017000">
        <property type="term" value="P:antibiotic biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1400.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.439.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.120.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014395">
        <property type="entry name" value="Pen/GL7ACA/AHL_acylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043147">
        <property type="entry name" value="Penicillin_amidase_A-knob"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023343">
        <property type="entry name" value="Penicillin_amidase_dom1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043146">
        <property type="entry name" value="Penicillin_amidase_N_B-knob"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002692">
        <property type="entry name" value="S45"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34218">
        <property type="entry name" value="PTHR34218"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01804">
        <property type="entry name" value="Penicil_amidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001227">
        <property type="entry name" value="Pen_acylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="SSF56235"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0106">Calcium</keyword>
    <keyword evidence="2 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0614">Plasmid</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0865">Zymogen</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193038" evidence="6">
        <location>
            <begin position="29"/>
            <end position="812"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="4">
        <location>
            <position position="278"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="184"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="350"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="353"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR001227-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR001227-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABF12494.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="812" mass="90379" checksum="9E8BA8E3F466ABD5" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="101" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LBL1</accession>
    <name>Q1LBL1_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">DNA-binding transcriptional dual regulator, LysR family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">cynR</name>
        <name evidence="3" type="ordered locus">Rmet_5606</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the LysR transcriptional regulatory family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12465.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5606"/>
    <dbReference type="EnsemblBacteria" id="ABF12465">
        <property type="protein sequence ID" value="ABF12465"/>
        <property type="gene ID" value="Rmet_5606"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5606"/>
    <dbReference type="eggNOG" id="COG0583">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039613_6_0_4"/>
    <dbReference type="OMA" id="EQCIAGV"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd08425">
        <property type="entry name" value="PBP2_CynR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037403">
        <property type="entry name" value="CynR_PBP2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005119">
        <property type="entry name" value="LysR_subst-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000847">
        <property type="entry name" value="Tscrpt_reg_HTH_LysR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00126">
        <property type="entry name" value="HTH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03466">
        <property type="entry name" value="LysR_substrate"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00039">
        <property type="entry name" value="HTHLYSR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50931">
        <property type="entry name" value="HTH_LYSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH lysR-type" evidence="2">
        <location>
            <begin position="29"/>
            <end position="86"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF12465.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="328" mass="35731" checksum="5BD82C1E384E2CE9" modified="2006-05-30" version="1">MAPAWRRTAIGSKTGLLSSSLATPIVTTMLLRHVRYFLAVADAGNFTRAAEALHVSQPTLSQQIRQLEESLGAPLFDRSGRTVRLTDAGEAYYVHARRALQDLEAGRRAIHDVGELTRGNLRLAITPTFTPYLVAPLIVAFNSAYPNITLSLREMPQERVEPLLADDEVDIGIAFDDIHLPDLDATPLMEEPLAYMVGATHALARRRTALRADVLAAQSMAMLTREFATRGYIARYFGALGIEPRITFEANSVSAVLDAVRSGRHATVLPAAIAAGHPDLCVLKLELPLPPRRAALLQRRGAYRTAAARAFVAFALDEVNRRGSPAHG</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LBL8</accession>
    <name>Q1LBL8_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Catalase</fullName>
            <ecNumber evidence="3">1.11.1.6</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">katA</name>
        <name evidence="14" type="ordered locus">Rmet_5599</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="15">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="15">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 12">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the catalase family.</text>
    </comment>
    <dbReference type="EC" id="1.11.1.6" evidence="3"/>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12458.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5599"/>
    <dbReference type="EnsemblBacteria" id="ABF12458">
        <property type="protein sequence ID" value="ABF12458"/>
        <property type="gene ID" value="Rmet_5599"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5599"/>
    <dbReference type="eggNOG" id="COG0753">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010645_2_0_4"/>
    <dbReference type="OMA" id="WTCYVQV"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004096">
        <property type="term" value="F:catalase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042744">
        <property type="term" value="P:hydrogen peroxide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006979">
        <property type="term" value="P:response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd08156">
        <property type="entry name" value="catalase_clade_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018028">
        <property type="entry name" value="Catalase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040333">
        <property type="entry name" value="Catalase_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024708">
        <property type="entry name" value="Catalase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024711">
        <property type="entry name" value="Catalase_clade1/3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011614">
        <property type="entry name" value="Catalase_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010582">
        <property type="entry name" value="Catalase_immune_responsive"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020835">
        <property type="entry name" value="Catalase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11465">
        <property type="entry name" value="PTHR11465"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00199">
        <property type="entry name" value="Catalase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06628">
        <property type="entry name" value="Catalase-rel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038928">
        <property type="entry name" value="Catalase_clade1-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00067">
        <property type="entry name" value="CATALASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM01060">
        <property type="entry name" value="Catalase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56634">
        <property type="entry name" value="SSF56634"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00438">
        <property type="entry name" value="CATALASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51402">
        <property type="entry name" value="CATALASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 12" id="KW-0349">Heme</keyword>
    <keyword evidence="10" id="KW-0376">Hydrogen peroxide</keyword>
    <keyword evidence="9 12" id="KW-0408">Iron</keyword>
    <keyword evidence="7 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 14" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5 14" id="KW-0575">Peroxidase</keyword>
    <keyword evidence="14" id="KW-0614">Plasmid</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Catalase" evidence="13">
        <location>
            <begin position="5"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="active site" evidence="11">
        <location>
            <position position="52"/>
        </location>
    </feature>
    <feature type="active site" evidence="11">
        <location>
            <position position="125"/>
        </location>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="12">
        <location>
            <position position="335"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012314"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00014132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR038928-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR038928-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM01060"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ABF12458.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="481" mass="54458" checksum="D4FEAD1FB446F538" modified="2006-05-30" version="1">MNKLTTAFGAPVVDNQNVQTAGPRGPALLQDVWFLEKLAHFDREVIPERRMHAKGAGAYGTFTVTHDITKYTRANLFSQVGKKTDLFLRFSSVAGERGAADAERDIRGFAIKFYTEEGNWDLVGNNTPVFFLRDPLKFPDLNHAVKRDPRTGMRSAESNWDFWTGLPEALHQVTIVMSDRGIPRTWRHMHGFGSHTYSFINANMERFWVKFHFVTQQGIQNLTDEEAAEITGQDREYHQRDLFESIERGDFPRWKLCVQIMPEAEASKVPYHPFDLTKIWPKKDYPLIEVGVLELNRNPDNFFAEVEQASFAPSTIVPGVGFSPDKMLQGRLFSYGDAARYRVGVNHHQIPVNSPKAAKYVNSYHRDGTMRVDGNRGAAIGYEPNTKGEWAEQPDFREPPLTLEGTADHWNHREDGDYFSQVGALFRLMSPTQQQALFDNTARSIKGVSKHIQQTHIKHCTAADPAYGAGVAAAIEMIEAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LC55</accession>
    <name>Q1LC55_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Transcriptional regulator, TetR family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_5412</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12271.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519819.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5412"/>
    <dbReference type="EnsemblBacteria" id="ABF12271">
        <property type="protein sequence ID" value="ABF12271"/>
        <property type="gene ID" value="Rmet_5412"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5412"/>
    <dbReference type="eggNOG" id="COG1309">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1298907_0_0_4"/>
    <dbReference type="OMA" id="CHWYANG"/>
    <dbReference type="OrthoDB" id="2047542at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001647">
        <property type="entry name" value="HTH_TetR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036271">
        <property type="entry name" value="Tet_transcr_reg_TetR-rel_C_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00440">
        <property type="entry name" value="TetR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00455">
        <property type="entry name" value="HTHTETR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48498">
        <property type="entry name" value="SSF48498"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50977">
        <property type="entry name" value="HTH_TETR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH tetR-type" evidence="2">
        <location>
            <begin position="23"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="H-T-H motif" evidence="1">
        <location>
            <begin position="46"/>
            <end position="65"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF12271.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="212" mass="23694" checksum="7CE1D1E6BD843EA9" modified="2006-05-30" version="1">MSANETGDVVAIPNGTTKYRRGLETRNRVIALTKSLISEHGYSEVTLDQISAQVGVAKSSLLWHFGSKEMLLAEAAISLFQEIELALRPDDLEGRPLAERVDEIFDRVADYFTRNPETKGVVMALLFSANTPQSVREHIREGWNGHVRVLVEALGTPERPLSPDAARLLVAIFHGAYCHWYANGRTQPIGDYLHPARDLFLNWISAENASRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LCB6</accession>
    <name>Q1LCB6_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Transporter, major facilitator superfamily (MFS)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Rmet_5351</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="8">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="8">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12210.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519756.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5351"/>
    <dbReference type="EnsemblBacteria" id="ABF12210">
        <property type="protein sequence ID" value="ABF12210"/>
        <property type="gene ID" value="Rmet_5351"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5351"/>
    <dbReference type="eggNOG" id="COG2814">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_040020_1_0_4"/>
    <dbReference type="OMA" id="GYGLFWF"/>
    <dbReference type="OrthoDB" id="1345431at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="SSF103473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0614">Plasmid</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="148"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="174"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="214"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="253"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="285"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="308"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="344"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="372"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="domain" description="MFS" evidence="6">
        <location>
            <begin position="16"/>
            <end position="397"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABF12210.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="404" mass="41960" checksum="F296AF60894BBA50" modified="2006-05-30" version="1">MATRAPASTLRQIPSGIWMLGCVSLLMDISSEIIHSLLPVFMVTTLGASALAVGLIEGAAEATALIVKVFSGVLSDYLGKRKALAVLGYGLGALSKPLFAVAPGVGTILAARLSDRIGKGIRGAPRDALVADIAPAHLRGAAFGLRQALDTVGAFLGPLIAIGLMMLWANDFRAIFWVAVVPGVLSVALLAFGVKEPQTHTGDKRVNPIRRANLARLGPAYWGVVAVGTAFALARFSEAFLILRVQQEGLPLFMAPLVLVTMNAVFAVTAYPFGKLADNCSHDKLLVAGLAVLIVADTVLAVGSHWSIALVGVALWGLHMGMTQGLLATMVADAAPADLRGTAFGVFNLASGLALLAASVMAGWLWDRHGAAATFHAGAGFCVLTIALLIHRLRNRPASGAATR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LCL3</accession>
    <name>Q1LCL3_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Flagellar hook-associated protein 2</fullName>
            <shortName evidence="5">HAP2</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Flagellar cap protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">fliD2</name>
        <name evidence="8" type="ordered locus">Rmet_5254</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="9">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="9">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Required for morphogenesis and for the elongation of the flagellar filament by facilitating polymerization of the flagellin monomers at the tip of growing filament. Forms a capping structure, which prevents flagellin subunits (transported through the central channel of the flagellum) from leaking out without polymerization at the distal end.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2 5">Homopentamer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Secreted</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Bacterial flagellum</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the FliD family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12113.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519659.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5254"/>
    <dbReference type="EnsemblBacteria" id="ABF12113">
        <property type="protein sequence ID" value="ABF12113"/>
        <property type="gene ID" value="Rmet_5254"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5254"/>
    <dbReference type="eggNOG" id="COG1345">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015182_6_1_4"/>
    <dbReference type="OMA" id="GGRQQIW"/>
    <dbReference type="OrthoDB" id="883503at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009421">
        <property type="term" value="C:bacterial-type flagellum filament cap"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009424">
        <property type="term" value="C:bacterial-type flagellum hook"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007155">
        <property type="term" value="P:cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040026">
        <property type="entry name" value="FliD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010809">
        <property type="entry name" value="FliD_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003481">
        <property type="entry name" value="FliD_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30288">
        <property type="entry name" value="PTHR30288"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07195">
        <property type="entry name" value="FliD_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02465">
        <property type="entry name" value="FliD_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0975">Bacterial flagellum</keyword>
    <keyword evidence="8" id="KW-0966">Cell projection</keyword>
    <keyword evidence="8" id="KW-0969">Cilium</keyword>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8" id="KW-0282">Flagellum</keyword>
    <keyword evidence="8" id="KW-0614">Plasmid</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0964">Secreted</keyword>
    <feature type="domain" description="FliD_N" evidence="6">
        <location>
            <begin position="11"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="domain" description="FliD_C" evidence="7">
        <location>
            <begin position="250"/>
            <end position="470"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009764"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023143"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU362066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF02465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF07195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ABF12113.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="490" mass="49985" checksum="041825B1448C7745" modified="2006-05-30" version="1">MTTISNLGVGSGLDLSSLLDQLTTAEQAPLTAIKTQQSSYQTKLSAYGQLQSMLAAFQASANQLSNPTFFQSTTASASNTSVLSATGSTTAVPGTYSVNVTQLAQSQSVVSTGQASQTTAVGTGTIHIDFGAITGGTLDSNPASATYGKYTGATFTANSGSTGVDITIDSSNNTLQGVRDAINKANAGVTASIVNDGSGTPYRLVISSNATGATNSVRMSVNETDGGTGLSDLAAYDPAGTQNMQQTIAAQNAQLTVNNIAVQSKGNSVADAVPGVVLNLTQTGTSSVTVQRDNSSIVSGVQAFVAAYNNLQNTAASLSAYDPSTKTGSPLTGDSTLSIIQSQMRSVMNTPQSGNALTTLSQIGISFQNDGTLALDTTKLTSALNSNPGAVAGLFGNTDGVSGYGNQINKLVTGLTSSNGALTTATAGINNTLKDLSNQYDETQTRVDAVIANYKTQFTQLDVIMSQMQNTSSYLTQQFSAMNGTSSSKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="61" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LCT6</accession>
    <name>Q1LCT6_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_5181</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12040.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519586.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5181"/>
    <dbReference type="EnsemblBacteria" id="ABF12040">
        <property type="protein sequence ID" value="ABF12040"/>
        <property type="gene ID" value="Rmet_5181"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5181"/>
    <dbReference type="HOGENOM" id="CLU_1554004_0_0_4"/>
    <dbReference type="OMA" id="LMMICAC"/>
    <dbReference type="OrthoDB" id="1686855at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="62"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="91"/>
            <end position="113"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF12040.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="172" mass="18494" checksum="3C4DB3BD53A4BCED" modified="2006-05-30" version="1">MRTLLYFILKDVVTARLAQARLGELANTSALTVGPWFSQRDSQPVDGLPSIDVGQTTYREELATSGIFVGAIIAALAVLRYGVSVGTSATAISYVGAMTLGAMTGWWVGGLVGTRIGRLGLRRQKAQMTPDQLLMISSCDSNSKESTKQLIHELGGVAIDEHNDLMPNLRWA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LD28</accession>
    <name>Q1LD28_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">ABC-type dipeptide transport system, membrane component</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">dppB</name>
        <name evidence="7" type="ordered locus">Rmet_5089</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="8">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="8">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cell membrane</location>
            <topology evidence="5">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the binding-protein-dependent transport system permease family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11948.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008642556.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5089"/>
    <dbReference type="EnsemblBacteria" id="ABF11948">
        <property type="protein sequence ID" value="ABF11948"/>
        <property type="gene ID" value="Rmet_5089"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5089"/>
    <dbReference type="eggNOG" id="COG0601">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036879_0_1_4"/>
    <dbReference type="OMA" id="AIQLPWF"/>
    <dbReference type="OrthoDB" id="1479518at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045621">
        <property type="entry name" value="BPD_transp_1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19300">
        <property type="entry name" value="BPD_transp_1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="SSF161098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0614">Plasmid</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="7"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="98"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="133"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="175"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="233"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="279"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="6">
        <location>
            <begin position="94"/>
            <end position="302"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABF11948.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="311" mass="33547" checksum="25A70994D5171363" modified="2006-05-30" version="1">MGYLIRRLLATLPVMAVVAIVVFLLIHLSPGDPAALIAGDLATVEDIARLRATLGLDQPLWQQFGLWAGRLASGDLGTSIFTQMPVTELLAQRLEPTLSIAALTMLLTLLVSVPLGTLAAYRAGSWIDRLVMLFAVLAFSLPVFLVGYLLVYAFAIQLPWFPVQGYARLADGAGGWLRSLVLPCVNLALVYIALITRMTRATVLEVLHEDYIRTARAKGLGVLPVLGHALRNAAIPIATTVGTGIALLIGGVVVTETVFAIPGVGRLVVDSVQRHDYPVIQSVLLLSAGAYVLINLMIDLSYRLFDPRIKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LD85</accession>
    <name>Q1LD85_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Putative lipase/acylhydrolase, GDSL-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Rmet_5029</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="5">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="5">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11891.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519440.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5029"/>
    <dbReference type="EnsemblBacteria" id="ABF11891">
        <property type="protein sequence ID" value="ABF11891"/>
        <property type="gene ID" value="Rmet_5029"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5029"/>
    <dbReference type="eggNOG" id="COG2755">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_616586_0_0_4"/>
    <dbReference type="OMA" id="QISAHRI"/>
    <dbReference type="OrthoDB" id="1984631at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016788">
        <property type="term" value="F:hydrolase activity, acting on ester bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013830">
        <property type="entry name" value="SGNH_hydro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036514">
        <property type="entry name" value="SGNH_hydro_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13472">
        <property type="entry name" value="Lipase_GDSL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0614">Plasmid</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193368" evidence="2">
        <location>
            <begin position="24"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="domain" description="SGNH_hydro" evidence="3">
        <location>
            <begin position="252"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="27"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="83"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="28"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="47"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="90"/>
            <end position="113"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF13472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF11891.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="444" mass="46221" checksum="17F9876874C4617A" modified="2006-05-30" version="1">MKSKLAALSMALAVGLVSGCGGGGGDGTTQASGGSSGTASPAAPGTTPSTPVTPVTPPTTTNPPATVTELQTTKALLKSALADGGAPTTMTSPPIITAGTANMPSTIKDSTLVPPNDPSLRYIGAMETAGTAFPDDLLVKNTAVNYGGLGRDSNVYYIEFVTDAPTFEILVKGNYQISAHRILVDGQYASTTPVAYPDDGNLYLTRVDFQGARKSRKIRIETPNMRFGGLRIAPGDSVTAPPVVPKVRAVILGDSITEGMAGLGYSFDNYAAKLGYLVGWNDMYQSGVGSTGYLAAPAPKLKFRDRVATDVYPFKPHVVVIAGGLNDACTSTDEFKAEATALFDDVQKNLPNTVVFVVGPWSPGCELQAHQDAIKAAVGTRANFYFIDNMAEQWQTGTGNVANPKGDGNSDIYISADGVHPTPAGHIYLAGKMADAVRKVVSTF</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LDB9</accession>
    <name>Q1LDB9_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">CENP-V/GFA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Rmet_4995</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="6">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="6">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the Gfa family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11857.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4995"/>
    <dbReference type="EnsemblBacteria" id="ABF11857">
        <property type="protein sequence ID" value="ABF11857"/>
        <property type="gene ID" value="Rmet_4995"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4995"/>
    <dbReference type="eggNOG" id="COG3791">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1288003_0_0_4"/>
    <dbReference type="OMA" id="AMGYCHC"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016846">
        <property type="term" value="F:carbon-sulfur lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006913">
        <property type="entry name" value="CENP-V/GFA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011057">
        <property type="entry name" value="Mss4-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04828">
        <property type="entry name" value="GFA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51316">
        <property type="entry name" value="SSF51316"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51891">
        <property type="entry name" value="CENP_V_GFA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0614">Plasmid</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CENP-V/GFA" evidence="4">
        <location>
            <begin position="69"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="25"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="30"/>
            <end position="48"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005495"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51891"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABF11857.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="214" mass="23225" checksum="70D1501936E6610D" modified="2006-05-30" version="1">MSQPLRWPTAATVLFLGALRRFAGPGSDSEQATRDRHHGGKRTSSLEAHRSEASVNLRCAAAYNPGAQLGGEMTVPCSVQCLCGAVNIKLNGQPQARANCHCRSCQDFYGTSMLSATAWLAEQVTVASGSVSSFSHPSKDLSRTFCSGCGETVFGTNRLGMRVLPNTIFARAAGGEVPEEMRPTMHLFYRHRIIDIQDELPKYLDGWDGPTYQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="92" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LDC7</accession>
    <name>Q1LDC7_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Putative sensor signal transduction histidine kinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Rmet_4987</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="5">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="5">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11849.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4987"/>
    <dbReference type="EnsemblBacteria" id="ABF11849">
        <property type="protein sequence ID" value="ABF11849"/>
        <property type="gene ID" value="Rmet_4987"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4987"/>
    <dbReference type="eggNOG" id="COG4585">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014388_2_0_4"/>
    <dbReference type="OMA" id="IWWRQRD"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="SSF55874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0614">Plasmid</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="210"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="243"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="274"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="309"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="334"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="360"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="393"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="3">
        <location>
            <begin position="557"/>
            <end position="647"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="420"/>
            <end position="458"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF11849.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="661" mass="72529" checksum="D8239C43E972918E" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="98" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LDF0</accession>
    <name>Q1LDF0_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Two component transcriptional regulator, LuxR family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_4964</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11826.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4964"/>
    <dbReference type="EnsemblBacteria" id="ABF11826">
        <property type="protein sequence ID" value="ABF11826"/>
        <property type="gene ID" value="Rmet_4964"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4964"/>
    <dbReference type="eggNOG" id="COG2197">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_90_1_4"/>
    <dbReference type="OMA" id="KAVWMAY"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06170">
        <property type="entry name" value="LuxR_C_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016032">
        <property type="entry name" value="Sig_transdc_resp-reg_C-effctor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000792">
        <property type="entry name" value="Tscrpt_reg_LuxR_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00196">
        <property type="entry name" value="GerE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00038">
        <property type="entry name" value="HTHLUXR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00421">
        <property type="entry name" value="HTH_LUXR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46894">
        <property type="entry name" value="SSF46894"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50043">
        <property type="entry name" value="HTH_LUXR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH luxR-type" evidence="2">
        <location>
            <begin position="156"/>
            <end position="221"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF11826.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="223" mass="24245" checksum="EE06DFE354A8FD56" modified="2006-05-30" version="1">MKKVRLCIASDRPAVCQALARRLESLPCIELVGNSGRAEEVLAITQRAGCDVVLQVREPESGLDQSALFLRLVDSTMAPPATLVVQADLTRRADVLTLLGSGVKNIVSALDDADAVAQAVLMAHMGIGSLSPRAMDVMWGSWRAAPRAVDEVRHSVDEIRHSLSRRELEVLRLYLAGVPISKIARDTGRSVNTVSAQKKSLMRKMNVQDHVQLVAMSIQYGLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LDF4</accession>
    <name>Q1LDF4_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Pili assembly chaperone</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">fimC</name>
        <name evidence="9" type="ordered locus">Rmet_4960</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="10">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="10">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Periplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the periplasmic pilus chaperone family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11822.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4960"/>
    <dbReference type="EnsemblBacteria" id="ABF11822">
        <property type="protein sequence ID" value="ABF11822"/>
        <property type="gene ID" value="Rmet_4960"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4960"/>
    <dbReference type="eggNOG" id="COG3121">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_070768_0_2_4"/>
    <dbReference type="OMA" id="YLINAWI"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030288">
        <property type="term" value="C:outer membrane-bounded periplasmic space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061077">
        <property type="term" value="P:chaperone-mediated protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008962">
        <property type="entry name" value="PapD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036316">
        <property type="entry name" value="Pili_assmbl_chap_C_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001829">
        <property type="entry name" value="Pili_assmbl_chaperone_bac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016148">
        <property type="entry name" value="Pili_assmbl_chaperone_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018046">
        <property type="entry name" value="Pili_assmbl_chaperone_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016147">
        <property type="entry name" value="Pili_assmbl_chaperone_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02753">
        <property type="entry name" value="PapD_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00345">
        <property type="entry name" value="PapD_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00969">
        <property type="entry name" value="CHAPERONPILI"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49354">
        <property type="entry name" value="SSF49354"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49584">
        <property type="entry name" value="SSF49584"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00635">
        <property type="entry name" value="PILI_CHAPERONE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0143">Chaperone</keyword>
    <keyword evidence="4" id="KW-0574">Periplasm</keyword>
    <keyword evidence="9" id="KW-0614">Plasmid</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="domain" description="PapD_N" evidence="7">
        <location>
            <begin position="12"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="domain" description="PapD_C" evidence="8">
        <location>
            <begin position="161"/>
            <end position="223"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007399"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022764"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ABF11822.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="234" mass="24642" checksum="FEE6C51775D87C83" modified="2006-05-30" version="1">MGLTGSAAHASVVLGGTRVILDEKDKEATIRASNEGNTPALIQTWVDKGDPKASPTSIEVPFTVMPPVARIDPSKGQTLRVLYTGEPLPTDKESVFWLNVLEIPPKATGDLAEANRLQLAFRTRVKLFYRPTGLVGTAAEAPSRVTWRIKPAGKTLALEATNPTGYHVSYAGMDVSAGGKTVRNQEGGMIGPGETKTFPLDGEAGLGANGSVHYRAINDWGGPIEGDAPVVPAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="95" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LDF5</accession>
    <name>Q1LDF5_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Fimbrial biogenesis outer membrane usher protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">fimD</name>
        <name evidence="14" type="ordered locus">Rmet_4959</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="15">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="15">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 10">Cell outer membrane</location>
            <topology evidence="2 10">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the fimbrial export usher family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11821.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519381.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4959"/>
    <dbReference type="EnsemblBacteria" id="ABF11821">
        <property type="protein sequence ID" value="ABF11821"/>
        <property type="gene ID" value="Rmet_4959"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4959"/>
    <dbReference type="eggNOG" id="COG3188">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009120_1_0_4"/>
    <dbReference type="OMA" id="QACKTID"/>
    <dbReference type="OrthoDB" id="120032at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009279">
        <property type="term" value="C:cell outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015473">
        <property type="term" value="F:fimbrial usher porin activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009297">
        <property type="term" value="P:pilus assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.2070">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.2610">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.410">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000015">
        <property type="entry name" value="Fimb_usher"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018030">
        <property type="entry name" value="Fimbrial_membr_usher_CS"/>
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    </dbReference>
    <dbReference type="InterPro" id="IPR025949">
        <property type="entry name" value="PapC-like_C"/>
    </dbReference>
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        <property type="entry name" value="PapC-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025885">
        <property type="entry name" value="PapC_N"/>
    </dbReference>
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        <property type="entry name" value="PapC_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30451">
        <property type="entry name" value="PTHR30451"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13953">
        <property type="entry name" value="PapC_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13954">
        <property type="entry name" value="PapC_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00577">
        <property type="entry name" value="Usher"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF141729">
        <property type="entry name" value="SSF141729"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01151">
        <property type="entry name" value="FIMBRIAL_USHER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 10" id="KW-0998">Cell outer membrane</keyword>
    <keyword evidence="10" id="KW-1029">Fimbrium biogenesis</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-0614">Plasmid</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 11" id="KW-0732">Signal</keyword>
    <keyword evidence="6 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1134">Transmembrane beta strand</keyword>
    <keyword evidence="4 10" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193344" evidence="11">
        <location>
            <begin position="37"/>
            <end position="867"/>
        </location>
    </feature>
    <feature type="domain" description="PapC_N" evidence="13">
        <location>
            <begin position="51"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="domain" description="PapC_C" evidence="12">
        <location>
            <begin position="783"/>
            <end position="847"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004571"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU003884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF13953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF13954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ABF11821.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="867" mass="91984" checksum="0732F0C99DB00CCD" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LE18</accession>
    <name>Q1LE18_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">AMP-dependent synthetase and ligase (Putative o-succinylbenzoate--CoA synthetase)</fullName>
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        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Rmet_4746</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="5">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="5">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
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    <dbReference type="EMBL" id="CP000353">
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4746"/>
    <dbReference type="EnsemblBacteria" id="ABF11608">
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        <property type="gene ID" value="Rmet_4746"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4746"/>
    <dbReference type="eggNOG" id="COG0318">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000022_59_0_4"/>
    <dbReference type="OMA" id="CEQYISV"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008756">
        <property type="term" value="F:o-succinylbenzoate-CoA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025110">
        <property type="entry name" value="AMP-bd_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045851">
        <property type="entry name" value="AMP-bd_C_sf"/>
    </dbReference>
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        <property type="entry name" value="AMP-binding_CS"/>
    </dbReference>
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        <property type="entry name" value="AMP-dep_Synth/Lig"/>
    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00455">
        <property type="entry name" value="AMP_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0436">Ligase</keyword>
    <keyword evidence="4" id="KW-0614">Plasmid</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="78"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="domain" description="AMP-binding_C" evidence="3">
        <location>
            <begin position="513"/>
            <end position="589"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
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        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF13193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF11608.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="601" mass="65663" checksum="368F9FC202860AE2" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="63" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LE75</accession>
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        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_4689</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11551.1"/>
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    </dbReference>
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    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4689"/>
    <dbReference type="EnsemblBacteria" id="ABF11551">
        <property type="protein sequence ID" value="ABF11551"/>
        <property type="gene ID" value="Rmet_4689"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4689"/>
    <dbReference type="eggNOG" id="ENOG502Z964">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005209_0_0_4"/>
    <dbReference type="OMA" id="VYLRRPW"/>
    <dbReference type="OrthoDB" id="732410at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF11551.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="1560" mass="167635" checksum="350DCCBAC2585CDC" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LEJ0</accession>
    <name>Q1LEJ0_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Acyl-CoA dehydrogenase protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Rmet_4574</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
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        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="9">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="9">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the acyl-CoA dehydrogenase family.</text>
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    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11436.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519023.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4574"/>
    <dbReference type="EnsemblBacteria" id="ABF11436">
        <property type="protein sequence ID" value="ABF11436"/>
        <property type="gene ID" value="Rmet_4574"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4574"/>
    <dbReference type="eggNOG" id="COG1960">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018204_9_0_4"/>
    <dbReference type="OMA" id="GIWMLGP"/>
    <dbReference type="OrthoDB" id="760677at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016627">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-CH group of donors"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006091">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046373">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036250">
        <property type="entry name" value="AcylCo_DH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009075">
        <property type="entry name" value="AcylCo_DH/oxidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013786">
        <property type="entry name" value="AcylCoA_DH/ox_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037069">
        <property type="entry name" value="AcylCoA_DH/ox_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009100">
        <property type="entry name" value="AcylCoA_DH/oxidase_NM_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00441">
        <property type="entry name" value="Acyl-CoA_dh_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02770">
        <property type="entry name" value="Acyl-CoA_dh_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02771">
        <property type="entry name" value="Acyl-CoA_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47203">
        <property type="entry name" value="SSF47203"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56645">
        <property type="entry name" value="SSF56645"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0274">FAD</keyword>
    <keyword evidence="3" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="8" id="KW-0614">Plasmid</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Acyl-CoA_dh_N" evidence="7">
        <location>
            <begin position="5"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA_dh_M" evidence="6">
        <location>
            <begin position="130"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA_dh_1" evidence="5">
        <location>
            <begin position="236"/>
            <end position="389"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF02770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF02771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ABF11436.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="394" mass="44054" checksum="DF5F1E8301AF065C" modified="2006-05-30" version="1">MDGLETFRQEAHAWLEANCPAEMRQPVRNEDDICWGGRKFRYQSDAQRLWLQRMAEKGWTVPEWPTQYGGGGLSPAQARVLRAEMARLNCRTPLQSFGISMLGPALLKYGTEAQKQGHLPKIARGEIRWCQGYSEPNAGSDLAALQTRAEERDGHFIVNGQKIWTSYADKADWIFCLVRTDFQAQKHTGISFLLFDMETPGVSTRPIVLISGKSPFCETFFDDVKVPCHQLVGEPNRGWEIAKYLLTHEREMISAIGSRGFREPMGRHAAKVIGVDAQRRLDDPMLRAQIAQFEIDEAAFAAAGERARDLAKQGEGMAAFSSVLKYYGAELNKRRYELLMSVGGTDALEWEGERSQDGALARSWLRTKANSIEGGTSEVQLNIVAKRLLGLPGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="61" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LES3</accession>
    <name>Q1LES3_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_4488</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11353.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518951.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4488"/>
    <dbReference type="EnsemblBacteria" id="ABF11353">
        <property type="protein sequence ID" value="ABF11353"/>
        <property type="gene ID" value="Rmet_4488"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4488"/>
    <dbReference type="eggNOG" id="ENOG5032QU2">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_070321_0_0_4"/>
    <dbReference type="OMA" id="KGYCHIR"/>
    <dbReference type="OrthoDB" id="1818215at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF11353.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="288" mass="33126" checksum="335CB8CB5B2D0923" modified="2006-05-30" version="1">MDEAIVGQDWETGEVFREGITVRVRHMTNGQVEINGFPTTAWRRINDARLRGQQLGTKRGESEHRERNEDISVRRATQQIRLRCKQICANSMITLTTRENIQEIERFYPLCKEFFRRMRLHGEFHYVAVPERQKRGAWHLHVACQGKVAKRLIIAVWLRVVGGKGNGYCHVSNPGRSDSQRGNGKRWESHRLAAYISKYIAKDVSGRELNDKRYWTSRGIVVPEKQDWGTWIDHPSMYDAARPVLEYLQGVAGLDDLVAHISVKGNSFWFATGPRFFGPMPPAIEPVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LEU9</accession>
    <name>Q1LEU9_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Histone-like nucleoid-structuring protein H-NS</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">hns</name>
        <name evidence="1" type="ordered locus">Rmet_4462</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11327.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518930.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF11327">
        <property type="protein sequence ID" value="ABF11327"/>
        <property type="gene ID" value="Rmet_4462"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4462"/>
    <dbReference type="eggNOG" id="COG2916">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_117503_8_1_4"/>
    <dbReference type="OMA" id="YPDWLQR"/>
    <dbReference type="OrthoDB" id="1911889at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030527">
        <property type="term" value="F:structural constituent of chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.430.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037150">
        <property type="entry name" value="H-NS_C_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001801">
        <property type="entry name" value="Histone_HNS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00816">
        <property type="entry name" value="Histone_HNS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF11327.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="99" mass="10996" checksum="D92BBA07B345EA63" modified="2006-05-30" version="1">MSESESAKQAAVVWVHEQMERHGLTFEDLVEAGCFADAHIEVADQADAVAKAATPTPEVPPRVMYRNAMGQTWDGNGEYPDWLQRAVNAGQSIDFYRVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LEW5</accession>
    <name>Q1LEW5_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Acyl-CoA transferase/carnitine dehydratase /bile acid-inducible protein F</fullName>
            <ecNumber evidence="1">2.8.3.16</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">caiB</name>
        <name evidence="1" type="ordered locus">Rmet_4446</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EC" id="2.8.3.16" evidence="1"/>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11311.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008647518.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4446"/>
    <dbReference type="EnsemblBacteria" id="ABF11311">
        <property type="protein sequence ID" value="ABF11311"/>
        <property type="gene ID" value="Rmet_4446"/>
    </dbReference>
    <dbReference type="GeneID" id="60825909"/>
    <dbReference type="KEGG" id="rme:Rmet_4446"/>
    <dbReference type="eggNOG" id="COG1804">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033975_0_0_4"/>
    <dbReference type="OMA" id="PIFARHD"/>
    <dbReference type="OrthoDB" id="969868at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033608">
        <property type="term" value="F:formyl-CoA transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1540.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10540">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003673">
        <property type="entry name" value="CoA-Trfase_fam_III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044855">
        <property type="entry name" value="CoA-Trfase_III_dom3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023606">
        <property type="entry name" value="CoA-Trfase_III_dom_1_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02515">
        <property type="entry name" value="CoA_transf_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89796">
        <property type="entry name" value="SSF89796"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF11311.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="393" mass="42566" checksum="5C0D4146B5E962B3" modified="2006-05-30" version="1">MGSGPLSRFTVLDLTRVRSGPAAVRQFADWGANVIKIEEPGLAPDDRPGGSAQVADYQNTHRNKQSITLNLKHPEGRALFLDLVRKADVVVENYRPSVKFKLGIDYESLRKVNPRIVYGSVSGFGQDGPYHDRPGLDQIAQGISGMMSVTGEPGRGPMRAGIPVADLTAGLFCAIGVMVALLEREVSGQGQWVQTSLLQSQLWMMDFQAMRWLMNGEVPGQSGNDHPTSSPTGVFPTADGHMNIAAMGNDIFARLCNVLGAPELVQDERFRTVPLRARHRAALGAEIAERTRTRTTQEWIARMNEEGVPCGPILNMKEAFADPQVEHLRMSQPIDHPTLGPISVVGQAVTLSRTPMGKLAPAPESGEHNLDVYRALLGLDAAKVEALAAQGVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-10-07" version="53" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LF10</accession>
    <name>Q1LF10_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_4401</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11266.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF11266">
        <property type="protein sequence ID" value="ABF11266"/>
        <property type="gene ID" value="Rmet_4401"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4401"/>
    <dbReference type="HOGENOM" id="CLU_1766488_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021945">
        <property type="entry name" value="DUF3562"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12085">
        <property type="entry name" value="DUF3562"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="55"/>
            <end position="86"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF11266.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="147" mass="16189" checksum="E7EEC53E18E95D9D" modified="2006-05-30" version="1">MPTAGKQNTTTPCVESKGNRRRGLSRVLGHMQYVGCTWNACYSVYTRQTPHREGPAMYLAPGTEPDRSSPAIPSASGGGSMRRPASGEHAIDRIAELYGAPRKLVEREFWDAWCSLSSDARILDYVVVLAERRVIKALRGRYAAGPH</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LF32</accession>
    <name>Q1LF32_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Acyl-carrier-protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">acpP2</name>
        <name evidence="3" type="ordered locus">Rmet_4379</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11244.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008652282.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4379"/>
    <dbReference type="EnsemblBacteria" id="ABF11244">
        <property type="protein sequence ID" value="ABF11244"/>
        <property type="gene ID" value="Rmet_4379"/>
    </dbReference>
    <dbReference type="GeneID" id="60825848"/>
    <dbReference type="KEGG" id="rme:Rmet_4379"/>
    <dbReference type="eggNOG" id="COG0236">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_108696_5_4_4"/>
    <dbReference type="OMA" id="QTREDIF"/>
    <dbReference type="OrthoDB" id="1943389at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006633">
        <property type="term" value="P:fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1200.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036736">
        <property type="entry name" value="ACP-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003231">
        <property type="entry name" value="Acyl_carrier"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009081">
        <property type="entry name" value="PP-bd_ACP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20863">
        <property type="entry name" value="PTHR20863"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00550">
        <property type="entry name" value="PP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47336">
        <property type="entry name" value="SSF47336"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50075">
        <property type="entry name" value="CARRIER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0596">Phosphopantetheine</keyword>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Carrier" evidence="2">
        <location>
            <begin position="4"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="modified residue" description="O-(pantetheine 4'-phosphoryl)serine" evidence="1">
        <location>
            <position position="39"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00258"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF11244.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="85" mass="9490" checksum="5B084A6BFA52B366" modified="2006-05-30" version="1">MTHDEIFTRVAGVLEQMFEIDPARITLDARLYDDLDIDSIDAVDLLVKMKPLMSRPIRAEQFKSVKTVGDVVRTLASLIDSPATA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LF44</accession>
    <name>Q1LF44_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Transcriptional regulator, LysR family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Rmet_4367</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="6">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="6">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the LysR transcriptional regulatory family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11232.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518849.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4367"/>
    <dbReference type="EnsemblBacteria" id="ABF11232">
        <property type="protein sequence ID" value="ABF11232"/>
        <property type="gene ID" value="Rmet_4367"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4367"/>
    <dbReference type="eggNOG" id="COG0583">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039613_6_0_4"/>
    <dbReference type="OMA" id="NAAAIWD"/>
    <dbReference type="OrthoDB" id="990666at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005044">
        <property type="term" value="F:scavenger receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005119">
        <property type="entry name" value="LysR_subst-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001190">
        <property type="entry name" value="SRCR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000847">
        <property type="entry name" value="Tscrpt_reg_HTH_LysR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00126">
        <property type="entry name" value="HTH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03466">
        <property type="entry name" value="LysR_substrate"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00039">
        <property type="entry name" value="HTHLYSR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50931">
        <property type="entry name" value="HTH_LYSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50287">
        <property type="entry name" value="SRCR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0614">Plasmid</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="222"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="domain" description="HTH lysR-type" evidence="4">
        <location>
            <begin position="1"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="domain" description="SRCR" evidence="3">
        <location>
            <begin position="81"/>
            <end position="160"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50287"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABF11232.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="309" mass="33307" checksum="1C2FF93AF4D40176" modified="2006-05-30" version="1">MNVSLPQLKAFAAVARQKSFTRAAAELGLTQSAISRSVRELEEEIDQRLFDRTTRQVELTDAGEMLSQRICHLVEEVEQTLRDSHGTGKPCEGTVQIATDPVLSSMTLPMWVAGCRKAWPGIGITLRDCSHECVLQRVRSGEVDFGIASNPAPCDDLYCEPLCVDPFQAVLPAGHPLAARESLGWGELGDASVLTLDQSSGIQPAVERALAAYRVRPGSLQVLGHFAAVFGMVAMGLGIGVVPSRAQAAGIAPSAVLRPLRPQVTSNVMLVRRKARSLKPNAAAVWDNLRAQEYERGDYGNQGLLKKVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="97" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LFA8</accession>
    <name>Q1LFA8_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Ferredoxin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">Rmet_4302</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="11">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="11">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[3Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:21137"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the succinate dehydrogenase/fumarate reductase iron-sulfur protein family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11168.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518795.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4302"/>
    <dbReference type="EnsemblBacteria" id="ABF11168">
        <property type="protein sequence ID" value="ABF11168"/>
        <property type="gene ID" value="Rmet_4302"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4302"/>
    <dbReference type="eggNOG" id="COG1152">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_089211_0_0_4"/>
    <dbReference type="OMA" id="CDRACPK"/>
    <dbReference type="OrthoDB" id="1605514at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051538">
        <property type="term" value="F:3 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00207">
        <property type="entry name" value="fer2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1060.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036010">
        <property type="entry name" value="2Fe-2S_ferredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001041">
        <property type="entry name" value="2Fe-2S_ferredoxin-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012675">
        <property type="entry name" value="Beta-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009051">
        <property type="entry name" value="Helical_ferredxn"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54292">
        <property type="entry name" value="SSF54292"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51085">
        <property type="entry name" value="2FE2S_FER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0003">3Fe-4S</keyword>
    <keyword evidence="5" id="KW-0408">Iron</keyword>
    <keyword evidence="6" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0614">Plasmid</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0816">Tricarboxylic acid cycle</keyword>
    <feature type="domain" description="2Fe-2S ferredoxin-type" evidence="8">
        <location>
            <begin position="1"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="9">
        <location>
            <begin position="141"/>
            <end position="169"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ABF11168.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="212" mass="23106" checksum="8284CDE5D473FA0F" modified="2006-05-30" version="1">MISLSIEGKQVEAPPNCSILQAFLHAGETLVEGVGCMGQGVCGSCRVLVRRAGKQEVITALACETLIEDGMQVAFLDFFTSPARHVYRLEDIEDSWQALQAISDIFPEAADCRHCSGCDRACPKGLDVQRGVNLAVEGNLSEAGHVFDECVMCNLCTLACPELIQPNHLGLFVRRMIASLTLRPANLMRRLREIERGDMTIDLDAPGAQPPH</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-12-02" version="69" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LFG8</accession>
    <name>Q1LFG8_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Alpha/beta hydrolase fold protein</fullName>
            <ecNumber evidence="2">3.1.1.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_4241</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EC" id="3.1.1.-" evidence="2"/>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11108.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4241"/>
    <dbReference type="ESTHER" id="ralme-q1lfg8">
        <property type="family name" value="6_AlphaBeta_hydrolase"/>
    </dbReference>
    <dbReference type="MEROPS" id="S33.010"/>
    <dbReference type="EnsemblBacteria" id="ABF11108">
        <property type="protein sequence ID" value="ABF11108"/>
        <property type="gene ID" value="Rmet_4241"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4241"/>
    <dbReference type="eggNOG" id="COG3208">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_083607_0_0_4"/>
    <dbReference type="OMA" id="MVHINRE"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12697">
        <property type="entry name" value="Abhydrolase_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="SSF53474"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AB hydrolase-1" evidence="1">
        <location>
            <begin position="64"/>
            <end position="277"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF11108.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="290" mass="31354" checksum="5CB3714DD28DC766" modified="2006-05-30" version="1">MSTGTSAPPTVRGDAINASLNANYAFPDTSTAAYDVDMQQNTNFTESSTDLLPGHVLGHPGALLLGLPGGGCSPAIFDYVKHDALQVHGVDWASWPGPWDLESLTTRLARVLQERKGPTLLAGHSLGGMFALLTTLKAGHKVNGLLLSNTGAHTAGHGDANLPDRVRNAWTEEASDAFLLSCFQCPPPVELWQKMREYMVHINREAFLEAVITLRQIDVRKRLSEIACPAVIAHGRHDQRRAMSFAQELADGIPRSALVWIDGGHTPMVDDPQAYHEALNTLVRLTGITG</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="54" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LFK9</accession>
    <name>Q1LFK9_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_4200</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11067.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518690.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4200"/>
    <dbReference type="EnsemblBacteria" id="ABF11067">
        <property type="protein sequence ID" value="ABF11067"/>
        <property type="gene ID" value="Rmet_4200"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4200"/>
    <dbReference type="HOGENOM" id="CLU_1904991_0_0_4"/>
    <dbReference type="OMA" id="VYWLDQT"/>
    <dbReference type="OrthoDB" id="1817217at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193491" evidence="1">
        <location>
            <begin position="24"/>
            <end position="133"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF11067.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="133" mass="14545" checksum="5A0E791DC658008D" modified="2006-05-30" version="1">MPRLRLSPLFAVAAAIVATVGTAACAPDSVRNYEAHGLNKYLNTIQDACADTRLGNHNLGEWLRSGTTDSDSDYVYWLDQTSRLYYSRINVPQYRDAIAGSMGGTKSNADALDCIVRHLPPDRPTQPPGGMFQ</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-12-12" modified="2022-08-03" version="92" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LFU5</accession>
    <name>PDXK_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Pyridoxine/pyridoxal/pyridoxamine kinase</fullName>
            <shortName evidence="1">PN/PL/PM kinase</shortName>
            <ecNumber evidence="1">2.7.1.35</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">B6-vitamer kinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">pdxK</name>
        <name type="ordered locus">Rmet_4114</name>
    </gene>
    <organism>
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid">
        <name>megaplasmid</name>
    </geneLocation>
    <reference key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">B6-vitamer kinase involved in the salvage pathway of pyridoxal 5'-phosphate (PLP). Catalyzes the phosphorylation of pyridoxine (PN), pyridoxal (PL), and pyridoxamine (PM), forming their respective 5'-phosphorylated esters, i.e. PNP, PLP and PMP.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + pyridoxal = ADP + H(+) + pyridoxal 5'-phosphate</text>
            <dbReference type="Rhea" id="RHEA:10224"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17310"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
            <dbReference type="EC" id="2.7.1.35"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + pyridoxine = ADP + H(+) + pyridoxine 5'-phosphate</text>
            <dbReference type="Rhea" id="RHEA:25108"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16709"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58589"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.35"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + pyridoxamine = ADP + H(+) + pyridoxamine 5'-phosphate</text>
            <dbReference type="Rhea" id="RHEA:25104"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57761"/>
            <dbReference type="ChEBI" id="CHEBI:58451"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.35"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor metabolism; pyridoxal 5'-phosphate salvage; pyridoxal 5'-phosphate from pyridoxal: step 1/1.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor metabolism; pyridoxal 5'-phosphate salvage; pyridoxine 5'-phosphate from pyridoxine: step 1/1.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor metabolism; pyridoxal 5'-phosphate salvage; pyridoxamine 5'-phosphate from pyridoxamine: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the pyridoxine kinase family. PdxK subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.35" evidence="1"/>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10981.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518609.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q1LFU5"/>
    <dbReference type="SMR" id="Q1LFU5"/>
    <dbReference type="STRING" id="266264.Rmet_4114"/>
    <dbReference type="EnsemblBacteria" id="ABF10981">
        <property type="protein sequence ID" value="ABF10981"/>
        <property type="gene ID" value="Rmet_4114"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4114"/>
    <dbReference type="eggNOG" id="COG2240">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046496_3_1_4"/>
    <dbReference type="OMA" id="ITYICDP"/>
    <dbReference type="OrthoDB" id="1480223at2"/>
    <dbReference type="UniPathway" id="UPA01068">
        <property type="reaction ID" value="UER00298"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA01068">
        <property type="reaction ID" value="UER00299"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA01068">
        <property type="reaction ID" value="UER00300"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008478">
        <property type="term" value="F:pyridoxal kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009443">
        <property type="term" value="P:pyridoxal 5'-phosphate salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01173">
        <property type="entry name" value="pyridoxal_pyridoxamine_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1190.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01638">
        <property type="entry name" value="PdxK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023479">
        <property type="entry name" value="PdxK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013749">
        <property type="entry name" value="PM/HMP-P_kinase-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004625">
        <property type="entry name" value="PyrdxlKinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029056">
        <property type="entry name" value="Ribokinase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10534">
        <property type="entry name" value="PTHR10534"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08543">
        <property type="entry name" value="Phos_pyr_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53613">
        <property type="entry name" value="SSF53613"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00687">
        <property type="entry name" value="pyridox_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0418">Kinase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0614">Plasmid</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <feature type="chain" id="PRO_0000268839" description="Pyridoxine/pyridoxal/pyridoxamine kinase">
        <location>
            <begin position="1"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="23"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="59"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="125"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="136"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="157"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="162"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="162"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="195"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="222"/>
            <end position="225"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="232"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="234"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01638"/>
        </source>
    </evidence>
    <sequence length="288" mass="30799" checksum="C2410E0E277F9EA2" modified="2006-05-30" version="1">MFGETGTGDALRPIFMDVVSVQSQVVYGHVGNNVALPTLRAHGLNVAAVPTVMFSNTPHYPTLHGGALPIDWFGGYLSDLSARGALQRLKAILVGYLGNPEQVAVLSRWINQVLAEHPDVQVIVDPVIGDHDTGIYVADGMVEAVRELLLPLAHGLTPNDFELGHLSGRSADSVEQVVAAARSLLTDRVQWMVVTSAAPGTWGHDDMKLLIVTRDDAQVLSHPRIPVSPKGTGDLFSAKLTARLLEGMPLAEAAASASDHVVTALEATRRAQSLELQLPSNPCITHRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LFY4</accession>
    <name>Q1LFY4_CUPMC</name>
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        <submittedName>
            <fullName evidence="3">Acyl-CoA dehydrogenase-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_4074</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10942.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518567.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4074"/>
    <dbReference type="EnsemblBacteria" id="ABF10942">
        <property type="protein sequence ID" value="ABF10942"/>
        <property type="gene ID" value="Rmet_4074"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4074"/>
    <dbReference type="eggNOG" id="COG1960">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018204_9_1_4"/>
    <dbReference type="OMA" id="DYMIAIF"/>
    <dbReference type="OrthoDB" id="760677at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003995">
        <property type="term" value="F:acyl-CoA dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.540.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.110.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006089">
        <property type="entry name" value="Acyl-CoA_DH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006091">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046373">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013786">
        <property type="entry name" value="AcylCoA_DH/ox_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037069">
        <property type="entry name" value="AcylCoA_DH/ox_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009100">
        <property type="entry name" value="AcylCoA_DH/oxidase_NM_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02770">
        <property type="entry name" value="Acyl-CoA_dh_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02771">
        <property type="entry name" value="Acyl-CoA_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56645">
        <property type="entry name" value="SSF56645"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00072">
        <property type="entry name" value="ACYL_COA_DH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Acyl-CoA_dh_N" evidence="2">
        <location>
            <begin position="15"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA_dh_M" evidence="1">
        <location>
            <begin position="131"/>
            <end position="227"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF10942.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="386" mass="41287" checksum="3D22A141063A2614" modified="2006-05-30" version="1">MSSLFGAFSLTSLPPEALAFRREVKAFLAEHLPDLPADVRARSWMGFDAAFSRALAQRGWVGVTLPARFGGAGLDAFSRFVLVEELLACGAPVSAHWIADRQSGPLILRYGSDAQREFYLPRICRGEAFFCIGMSEPNSGSDLASVATRAVPQPGGGWRLNGRKIWTTNADRCHYMLALVRTSGEAGDRQKGLSQVIVDLSLPGVTVRPIHDLAGDAHFSEVSFEDVLLPADALIGNEGAGWEQVNAELAFERSGPERLYSSVVLLDAWVAALRRLPAVRRDAVTIGGIVGRLATLRAMSVAVTARLAAGESPLVEAALVKDLGTGLEQSIGALAEAALGDDPELAMDAELYRTAAYVSQISPTYSLRGGTREILRGMIARGLGLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="98" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LG77</accession>
    <name>Q1LG77_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Transcriptional regulator, IclR family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Rmet_3981</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="6">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="6">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10849.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518489.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3981"/>
    <dbReference type="EnsemblBacteria" id="ABF10849">
        <property type="protein sequence ID" value="ABF10849"/>
        <property type="gene ID" value="Rmet_3981"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3981"/>
    <dbReference type="eggNOG" id="COG1414">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_062618_0_0_4"/>
    <dbReference type="OMA" id="IAQPLMQ"/>
    <dbReference type="OrthoDB" id="370329at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029016">
        <property type="entry name" value="GAF-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014757">
        <property type="entry name" value="Tscrpt_reg_IclR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005471">
        <property type="entry name" value="Tscrpt_reg_IclR_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09339">
        <property type="entry name" value="HTH_IclR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01614">
        <property type="entry name" value="IclR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00346">
        <property type="entry name" value="HTH_ICLR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51077">
        <property type="entry name" value="HTH_ICLR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51078">
        <property type="entry name" value="ICLR_ED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0614">Plasmid</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH iclR-type" evidence="3">
        <location>
            <begin position="32"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="domain" description="IclR-ED" evidence="4">
        <location>
            <begin position="95"/>
            <end position="278"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABF10849.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="280" mass="30172" checksum="7851BB7907AE6A0C" modified="2006-05-30" version="1">MKRTTSIPMPADTALVSADAARPAEDGDRNFVTALARGVELLRAFGPDDDFLGNAELSRRTGIPRPTVSRLTYTLASLGYLVYVEAQEKYRIGQAAMLPAQRYLSGAGIRDIAQPLMQSLAFATGCTVALAAPDRHDMVYLEVCQPRGALVMRLTPGSRLPMATSAIGKAWLAGLTSDRRGAVFAELERHHGARWASIRTKLERGLREHARRGFCMAHAEWDRSVSGAAAPVSLADGSEVLSINIGGASTRLSPEILEGNLGPRIRELADALAARLWRPG</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="109" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LG89</accession>
    <name>Q1LG89_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Histidine kinase</fullName>
            <ecNumber evidence="2">2.7.13.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">Rmet_3969</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="10">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="10">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.</text>
            <dbReference type="EC" id="2.7.13.3"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.7.13.3" evidence="2"/>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10837.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518476.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3969"/>
    <dbReference type="EnsemblBacteria" id="ABF10837">
        <property type="protein sequence ID" value="ABF10837"/>
        <property type="gene ID" value="Rmet_3969"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3969"/>
    <dbReference type="eggNOG" id="COG0643">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG0745">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG2198">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000650_2_1_4"/>
    <dbReference type="OMA" id="QLEACMR"/>
    <dbReference type="OrthoDB" id="305000at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006935">
        <property type="term" value="P:chemotaxis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00088">
        <property type="entry name" value="HPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.160">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004105">
        <property type="entry name" value="CheA-like_dim"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036061">
        <property type="entry name" value="CheW-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002545">
        <property type="entry name" value="CheW-lke_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036641">
        <property type="entry name" value="HPT_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004358">
        <property type="entry name" value="Sig_transdc_His_kin-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008207">
        <property type="entry name" value="Sig_transdc_His_kin_Hpt_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01584">
        <property type="entry name" value="CheW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01627">
        <property type="entry name" value="Hpt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00344">
        <property type="entry name" value="BCTRLSENSOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00260">
        <property type="entry name" value="CheW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01231">
        <property type="entry name" value="H-kinase_dim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00073">
        <property type="entry name" value="HPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47226">
        <property type="entry name" value="SSF47226"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50341">
        <property type="entry name" value="SSF50341"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="SSF55874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50851">
        <property type="entry name" value="CHEW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50894">
        <property type="entry name" value="HPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-0418">Kinase</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9" id="KW-0614">Plasmid</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="HPt" evidence="8">
        <location>
            <begin position="4"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="5">
        <location>
            <begin position="237"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="domain" description="CheW-like" evidence="7">
        <location>
            <begin position="488"/>
            <end position="622"/>
        </location>
    </feature>
    <feature type="domain" description="Response regulatory" evidence="6">
        <location>
            <begin position="646"/>
            <end position="762"/>
        </location>
    </feature>
    <feature type="modified residue" description="Phosphohistidine" evidence="3">
        <location>
            <position position="51"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="4">
        <location>
            <position position="695"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50851"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ABF10837.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="768" mass="83962" checksum="F74825812C2DFE1B" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="105" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LGK2</accession>
    <name>Q1LGK2_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="9">DL-methionine transporter subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">metN</name>
        <name evidence="9" type="ordered locus">Rmet_3856</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="10">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="10">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10724.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518371.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3856"/>
    <dbReference type="EnsemblBacteria" id="ABF10724">
        <property type="protein sequence ID" value="ABF10724"/>
        <property type="gene ID" value="Rmet_3856"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3856"/>
    <dbReference type="eggNOG" id="COG1135">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_22_4"/>
    <dbReference type="OMA" id="RWEEMIN"/>
    <dbReference type="OrthoDB" id="1058434at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042626">
        <property type="term" value="F:ATPase-coupled transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015821">
        <property type="term" value="P:methionine transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03258">
        <property type="entry name" value="ABC_MetN_methionine_transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026253">
        <property type="entry name" value="ABC_MetN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041701">
        <property type="entry name" value="MetN_ABC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43166:SF5">
        <property type="entry name" value="PTHR43166:SF5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="1" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-0614">Plasmid</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-1278">Translocase</keyword>
    <feature type="domain" description="ABC transporter" evidence="8">
        <location>
            <begin position="14"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="48"/>
            <end position="55"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ABF10724.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="274" mass="29873" checksum="BD888E376AF2FA5F" modified="2006-05-30" version="1">MHTVSQLHARDSHIVFEQVGKTYPGAATPALTDVSFSIGRGERFGIIGRSGAGKSTLLRTINMLERPDEGHVRVDGVDIGTLDEKGLVALRRRIGMIFQHFNLMSAKTVFENVALPLRVAGVGRAETASRVNELLALVGLADKANAYPAMLSGGQKQRVGIARALVHRPEILLCDEATSALDPETTEQILALLRDINRQFALTIVLITHDMAVIDEACDRVLVLDHGHAMEHGPVWRVFSEPKADATIALLRPLRKRLQAQAHSPEIVDRVANG</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LGX1</accession>
    <name>Q1LGX1_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Flagella basal body P-ring formation protein FlgA</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">flgA</name>
        <name evidence="6" type="ordered locus">Rmet_3733</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="7">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="7">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Involved in the assembly process of the P-ring formation. It may associate with FlgF on the rod constituting a structure essential for the P-ring assembly or may act as a modulator protein for the P-ring assembly.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 4">Periplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the FlgA family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10605.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518251.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3733"/>
    <dbReference type="EnsemblBacteria" id="ABF10605">
        <property type="protein sequence ID" value="ABF10605"/>
        <property type="gene ID" value="Rmet_3733"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3733"/>
    <dbReference type="eggNOG" id="COG1261">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_070510_2_1_4"/>
    <dbReference type="OMA" id="RYEIQVN"/>
    <dbReference type="OrthoDB" id="1521961at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042597">
        <property type="term" value="C:periplasmic space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044780">
        <property type="term" value="P:bacterial-type flagellum assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017585">
        <property type="entry name" value="Flag_basal_body_FlgA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039246">
        <property type="entry name" value="Flagellar_FlgA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041231">
        <property type="entry name" value="FlgA_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013974">
        <property type="entry name" value="SAF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36307">
        <property type="entry name" value="PTHR36307"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13144">
        <property type="entry name" value="ChapFlgA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17656">
        <property type="entry name" value="ChapFlgA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00858">
        <property type="entry name" value="SAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03170">
        <property type="entry name" value="flgA_cterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1005">Bacterial flagellum biogenesis</keyword>
    <keyword evidence="6" id="KW-0966">Cell projection</keyword>
    <keyword evidence="6" id="KW-0969">Cilium</keyword>
    <keyword evidence="6" id="KW-0282">Flagellum</keyword>
    <keyword evidence="3 4" id="KW-0574">Periplasm</keyword>
    <keyword evidence="6" id="KW-0614">Plasmid</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5005142739" description="Flagella basal body P-ring formation protein FlgA" evidence="4">
        <location>
            <begin position="25"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="domain" description="SAF" evidence="5">
        <location>
            <begin position="121"/>
            <end position="183"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022764"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU362063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00858"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABF10605.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="245" mass="25473" checksum="2B6F572207F77705" modified="2006-05-30" version="1">MNLYRQAAALLALAGIAMSQPVLAAPAVATPVTAQTPSTPFVDDPARVAVEQFLLRQAGGLPGKVSVQVGTPSGGRAPECVDPQPFLPPGAAPYGRVNVGLRCGGDRPWTRYMQARVSVLTDYYVAARAMGPGETITAADLEVRQGDLAMLPRAVVTDPSQVDGAVSANRIAAGSPMRTDLVRKPIAVKSGQTVNVTVEGDSFQLSSEGKVLTDAATGNMVQVRLRNGQVVSGLVRSGDTVVLQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LH24</accession>
    <name>Q1LH24_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Response regulator receiver domain protein (CheY-like)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_3680</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10552.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518203.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3680"/>
    <dbReference type="EnsemblBacteria" id="ABF10552">
        <property type="protein sequence ID" value="ABF10552"/>
        <property type="gene ID" value="Rmet_3680"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3680"/>
    <dbReference type="eggNOG" id="COG0745">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_69_17_4"/>
    <dbReference type="OMA" id="MHFTKPF"/>
    <dbReference type="OrthoDB" id="1729688at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Response regulatory" evidence="2">
        <location>
            <begin position="5"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="1">
        <location>
            <position position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF10552.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="127" mass="13578" checksum="6473451CAC198368" modified="2006-05-30" version="1">MSLLSILVVDDSPSLRRMTSACLRAGGYGVTEASDGKEAYEVAREGNFDMLVTDQVMPVMDGLSLIRALRAMPDYATVPMLMLSTEHDDAILERAREAGASGFLAKPFDPDELMASVDALLGPPNVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LH39</accession>
    <name>Q1LH39_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">M20_dimer domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Rmet_3665</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="5">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="5">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10537.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3665"/>
    <dbReference type="EnsemblBacteria" id="ABF10537">
        <property type="protein sequence ID" value="ABF10537"/>
        <property type="gene ID" value="Rmet_3665"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3665"/>
    <dbReference type="eggNOG" id="COG0624">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021802_11_2_4"/>
    <dbReference type="OMA" id="VVPPFFE"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001261">
        <property type="entry name" value="ArgE/DapE_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036264">
        <property type="entry name" value="Bact_exopeptidase_dim_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002933">
        <property type="entry name" value="Peptidase_M20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011650">
        <property type="entry name" value="Peptidase_M20_dimer"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07687">
        <property type="entry name" value="M20_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01546">
        <property type="entry name" value="Peptidase_M20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55031">
        <property type="entry name" value="SSF55031"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00758">
        <property type="entry name" value="ARGE_DAPE_CPG2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00759">
        <property type="entry name" value="ARGE_DAPE_CPG2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0614">Plasmid</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193490" description="M20_dimer domain-containing protein" evidence="2">
        <location>
            <begin position="28"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="domain" description="M20_dimer" evidence="3">
        <location>
            <begin position="231"/>
            <end position="376"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF07687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF10537.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="478" mass="52336" checksum="75F930C02612541F" modified="2006-05-30" version="1">MRIHRRPEAVRALVAAVLVTCAAGAVAQSLPASKAPPATLTPDQQRFHDIYKELVEINTTHSAGDSAQAARAMEKRLVEAGFSASDIQVFEPFPRKGNLVMRFKGTGARQPVLLLAHIDVVEAKREDWKTDPFQLQETNGYFTARGSIDDKAMASAFVSVLGQLKQEGFKPSRDIILALTSDEERGDVPSNGADWLVKNKPELLKAEFGINEGGGGELRDGKPVLHRVQVAEKMYTTYELEVRDVGGHSSVPTKSNPVYAMSEALSRLGAYQFPVKMADVTQTYFARSAPLAKGQLAEDMREVGTGRPSQAAIDRLSAIPFYNAQLRTTCVATMVNAGHAENALPQSAKAIVNCRILPHDDPADIDRQLKSALGNDKISVRYINKPLASPASPLDGALMKEVDALTKQMWDVPVIPAMSTGATDSRFMRNAGIRMYGVSGLFTEPSDMRAHGLDERIEIARLYDGREFLYRLVKRLSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-02-10" version="69" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LH62</accession>
    <name>Q1LH62_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_3642</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the YggT family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10514.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3642"/>
    <dbReference type="EnsemblBacteria" id="ABF10514">
        <property type="protein sequence ID" value="ABF10514"/>
        <property type="gene ID" value="Rmet_3642"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3642"/>
    <dbReference type="eggNOG" id="COG0762">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_089905_0_1_4"/>
    <dbReference type="OMA" id="MIDFSPM"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003425">
        <property type="entry name" value="CCB3/YggT"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02325">
        <property type="entry name" value="YGGT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="21"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="84"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="116"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="178"/>
            <end position="197"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF10514.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="202" mass="22452" checksum="7446D25728C95753" modified="2006-05-30" version="1">MAESVRLPTPYNRKTEIRPMFTEIILFLLDTVFTLFGMALLLRLWMQLSRLPSRNPVSQGVFQVTDWIVRPLRRIIPGLGGIDWATVFAAYLTAVAFLLCRALVLGADPLGFLPVILALGVLSMLKWGISMVMWVTLLMAVLSWVNPNSPLAAPIWHLTAPLLRPIQRVVPRLGGFDISPLILFVIAQILLMVIANVSRGVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LHA4</accession>
    <name>Q1LHA4_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative hydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_3600</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10472.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518015.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3600"/>
    <dbReference type="ESTHER" id="ralme-q1lha4">
        <property type="family name" value="Epoxide_hydrolase"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF10472">
        <property type="protein sequence ID" value="ABF10472"/>
        <property type="gene ID" value="Rmet_3600"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3600"/>
    <dbReference type="eggNOG" id="COG2267">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020336_13_2_4"/>
    <dbReference type="OMA" id="ASTDHWR"/>
    <dbReference type="OrthoDB" id="1119700at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000639">
        <property type="entry name" value="Epox_hydrolase-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12697">
        <property type="entry name" value="Abhydrolase_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00111">
        <property type="entry name" value="ABHYDROLASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00412">
        <property type="entry name" value="EPOXHYDRLASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="SSF53474"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AB hydrolase-1" evidence="1">
        <location>
            <begin position="70"/>
            <end position="311"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF10472.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="333" mass="37235" checksum="67BD1BB4576E108D" modified="2006-05-30" version="1">MNRAALSREAALQSPPGAFTRWFGQGRIGLFSLSRETLAHRHALPASRWVSVMGTLVHYTEEGVPEGEPLVLIHGFGASLHTWEGLMPMLRRRYRVVRLDLAPFGLTGPLRDAHGRIMTMDVDVYRAFIDGFLAAVNISCATLIGNSLGGLIAWDLAARRPSLVNRLVLVDAAGFPMKLPIYIDLFRHAMVRWSAPWMLPEWIIRAATRDVYGDASHVPEATFRRYVDFFYAAGSREAVGRMVPKLDFDGLDTERLRGVRVPTLVLWGDRDRWIPPAHAAEFAARIPDVTLRRYAGLGHIPMEEDPPRVAADLLPFLDGAREARPSFSKEFAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-04-07" version="100" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LHE0</accession>
    <name>Q1LHE0_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 8">Methionyl-tRNA formyltransferase</fullName>
            <ecNumber evidence="4 8">2.1.2.9</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8 11" type="primary">fmt</name>
        <name evidence="11" type="ordered locus">Rmet_3564</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2 8">Attaches a formyl group to the free amino group of methionyl-tRNA(fMet). The formyl group appears to play a dual role in the initiator identity of N-formylmethionyl-tRNA by promoting its recognition by IF2 and preventing the misappropriation of this tRNA by the elongation apparatus.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 8">
            <text>(6S)-10-formyltetrahydrofolate + L-methionyl-tRNA(fMet) = (6S)-5,6,7,8-tetrahydrofolate + H(+) + N-formyl-L-methionyl-tRNA(fMet)</text>
            <dbReference type="Rhea" id="RHEA:24380"/>
            <dbReference type="Rhea" id="RHEA-COMP:9952"/>
            <dbReference type="Rhea" id="RHEA-COMP:9953"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57453"/>
            <dbReference type="ChEBI" id="CHEBI:57454"/>
            <dbReference type="ChEBI" id="CHEBI:78530"/>
            <dbReference type="ChEBI" id="CHEBI:78844"/>
            <dbReference type="EC" id="2.1.2.9"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="3 8">Belongs to the Fmt family.</text>
    </comment>
    <dbReference type="EC" id="2.1.2.9" evidence="4 8"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10436.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3564"/>
    <dbReference type="EnsemblBacteria" id="ABF10436">
        <property type="protein sequence ID" value="ABF10436"/>
        <property type="gene ID" value="Rmet_3564"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3564"/>
    <dbReference type="eggNOG" id="COG0223">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033347_1_2_4"/>
    <dbReference type="OMA" id="CCPVVAY"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004479">
        <property type="term" value="F:methionyl-tRNA formyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd08646">
        <property type="entry name" value="FMT_core_Met-tRNA-FMT_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd08704">
        <property type="entry name" value="Met_tRNA_FMT_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.25.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00182">
        <property type="entry name" value="Formyl_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005794">
        <property type="entry name" value="Fmt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005793">
        <property type="entry name" value="Formyl_trans_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037022">
        <property type="entry name" value="Formyl_trans_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002376">
        <property type="entry name" value="Formyl_transf_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036477">
        <property type="entry name" value="Formyl_transf_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011034">
        <property type="entry name" value="Formyl_transferase-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001555">
        <property type="entry name" value="GART_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044135">
        <property type="entry name" value="Met-tRNA-FMT_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041711">
        <property type="entry name" value="Met-tRNA-FMT_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02911">
        <property type="entry name" value="Formyl_trans_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00551">
        <property type="entry name" value="Formyl_trans_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50486">
        <property type="entry name" value="SSF50486"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53328">
        <property type="entry name" value="SSF53328"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00460">
        <property type="entry name" value="fmt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00373">
        <property type="entry name" value="GART"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 8 11" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Formyl_trans_N" evidence="9">
        <location>
            <begin position="20"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="domain" description="Formyl_trans_C" evidence="10">
        <location>
            <begin position="231"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="region of interest" description="Tetrahydrofolate (THF) binding" evidence="8">
        <location>
            <begin position="136"/>
            <end position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00016014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF02911"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ABF10436.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="344" mass="36056" checksum="11B915473F566A54" modified="2006-05-30" version="1">MRPVSFSLRNIVMTQAQPLRVAFAGTPEFARVALEAIHAAGFPVVAVLTQPDRPAGRGMQLQASPVKQFAVEAGLGPVLQPRSLRRQGKYPEEAGAAVDTLAEIAPDVMVVAAYGLILPTEVLELPRHGCLNIHASLLPRWRGAAPIHRAIEAGDPETGITLMQMDEGLDTGAMLSRESTPIGPQDSTGTLHDTLAALGGRMIVDALRQLAAGQPLTATPQPEAGVTYAEKIAKDEAPLDLTRPAIELANQIRAFNPFPGATVQVGDTIIKCWQALPLAAATSLPHPPGTILAADTAGVIIACGNGSALQVTELQKPGGRRQPAQQFLQAMPLPPGTRCLIAGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="114" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LHK5</accession>
    <name>Q1LHK5_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">ATP synthase subunit c</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">ATP synthase F(0) sector subunit c</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">F-type ATPase subunit c</fullName>
            <shortName evidence="13">F-ATPase subunit c</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">Lipid-binding protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13 15" type="primary">atpE</name>
        <name evidence="15" type="ordered locus">Rmet_3499</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12 13">F(1)F(0) ATP synthase produces ATP from ADP in the presence of a proton or sodium gradient. F-type ATPases consist of two structural domains, F(1) containing the extramembraneous catalytic core and F(0) containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation.</text>
    </comment>
    <comment type="function">
        <text evidence="13">Key component of the F(0) channel; it plays a direct role in translocation across the membrane. A homomeric c-ring of between 10-14 subunits forms the central stalk rotor element with the F(1) delta and epsilon subunits.</text>
    </comment>
    <comment type="subunit">
        <text evidence="13">F-type ATPases have 2 components, F(1) - the catalytic core - and F(0) - the membrane proton channel. F(1) has five subunits: alpha(3), beta(3), gamma(1), delta(1), epsilon(1). F(0) has three main subunits: a(1), b(2) and c(10-14). The alpha and beta chains form an alternating ring which encloses part of the gamma chain. F(1) is attached to F(0) by a central stalk formed by the gamma and epsilon chains, while a peripheral stalk is formed by the delta and b chains.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="13">Cell inner membrane</location>
            <topology evidence="13">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the ATPase C chain family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10371.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008650162.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3499"/>
    <dbReference type="EnsemblBacteria" id="ABF10371">
        <property type="protein sequence ID" value="ABF10371"/>
        <property type="gene ID" value="Rmet_3499"/>
    </dbReference>
    <dbReference type="GeneID" id="60822605"/>
    <dbReference type="KEGG" id="rme:Rmet_3499"/>
    <dbReference type="eggNOG" id="ENOG5032S3K">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_148047_1_0_4"/>
    <dbReference type="OMA" id="QPELMNE"/>
    <dbReference type="OrthoDB" id="2078221at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045263">
        <property type="term" value="C:proton-transporting ATP synthase complex, coupling factor F(o)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046933">
        <property type="term" value="F:proton-transporting ATP synthase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.20.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01396">
        <property type="entry name" value="ATP_synth_c_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005953">
        <property type="entry name" value="ATP_synth_csu_bac/chlpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000454">
        <property type="entry name" value="ATP_synth_F0_csu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020537">
        <property type="entry name" value="ATP_synth_F0_csu_DDCD_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038662">
        <property type="entry name" value="ATP_synth_F0_csu_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002379">
        <property type="entry name" value="ATPase_proteolipid_c-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035921">
        <property type="entry name" value="F/V-ATP_Csub_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00137">
        <property type="entry name" value="ATP-synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81333">
        <property type="entry name" value="SSF81333"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01260">
        <property type="entry name" value="ATP_synt_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00605">
        <property type="entry name" value="ATPASE_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 13" id="KW-0066">ATP synthesis</keyword>
    <keyword evidence="13" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="13" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4 13" id="KW-0138">CF(0)</keyword>
    <keyword evidence="6 13" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="15" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8 13" id="KW-0406">Ion transport</keyword>
    <keyword evidence="9 13" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="10 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 13" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 13" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="12"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="56"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="domain" description="ATP-synt_C" evidence="14">
        <location>
            <begin position="14"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="site" description="Reversibly protonated during proton transport" evidence="13">
        <location>
            <position position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022547"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023310"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00025198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01396"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="ABF10371.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="88" mass="9064" checksum="F1B345C774236B18" modified="2006-05-30" version="1">MQAYLANIQGLTAIGIGIIIGLGAIGACLGIALMGGKYIEACARQPELMNPLQTKMFLLAGLIDAAFLIGVGVAMLFAFANPLLSVIQ</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-10-31" modified="2022-08-03" version="110" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LHK8</accession>
    <name>ATPA_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">ATP synthase subunit alpha</fullName>
            <ecNumber evidence="1">7.1.2.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">ATP synthase F1 sector subunit alpha</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">F-ATPase subunit alpha</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">atpA</name>
        <name type="ordered locus">Rmet_3496</name>
    </gene>
    <organism>
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Produces ATP from ADP in the presence of a proton gradient across the membrane. The alpha chain is a regulatory subunit.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + 4 H(+)(in) + H2O = ADP + 5 H(+)(out) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:57720"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="7.1.2.2"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">F-type ATPases have 2 components, CF(1) - the catalytic core - and CF(0) - the membrane proton channel. CF(1) has five subunits: alpha(3), beta(3), gamma(1), delta(1), epsilon(1). CF(0) has three main subunits: a(1), b(2) and c(9-12). The alpha and beta chains form an alternating ring which encloses part of the gamma chain. CF(1) is attached to CF(0) by a central stalk formed by the gamma and epsilon chains, while a peripheral stalk is formed by the delta and b chains.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell inner membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the ATPase alpha/beta chains family.</text>
    </comment>
    <dbReference type="EC" id="7.1.2.2" evidence="1"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10368.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517923.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q1LHK8"/>
    <dbReference type="SMR" id="Q1LHK8"/>
    <dbReference type="STRING" id="266264.Rmet_3496"/>
    <dbReference type="PRIDE" id="Q1LHK8"/>
    <dbReference type="EnsemblBacteria" id="ABF10368">
        <property type="protein sequence ID" value="ABF10368"/>
        <property type="gene ID" value="Rmet_3496"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3496"/>
    <dbReference type="eggNOG" id="COG0056">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010091_2_1_4"/>
    <dbReference type="OMA" id="LQAPGVM"/>
    <dbReference type="OrthoDB" id="837522at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045261">
        <property type="term" value="C:proton-transporting ATP synthase complex, catalytic core F(1)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046933">
        <property type="term" value="F:proton-transporting ATP synthase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046961">
        <property type="term" value="F:proton-transporting ATPase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd18113">
        <property type="entry name" value="ATP-synt_F1_alpha_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01132">
        <property type="entry name" value="F1_ATPase_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.150.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01346">
        <property type="entry name" value="ATP_synth_alpha_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023366">
        <property type="entry name" value="ATP_synth_asu-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000793">
        <property type="entry name" value="ATP_synth_asu_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038376">
        <property type="entry name" value="ATP_synth_asu_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033732">
        <property type="entry name" value="ATP_synth_F1_a"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005294">
        <property type="entry name" value="ATP_synth_F1_asu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020003">
        <property type="entry name" value="ATPase_a/bsu_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004100">
        <property type="entry name" value="ATPase_F1/V1/A1_a/bsu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036121">
        <property type="entry name" value="ATPase_F1/V1/A1_a/bsu_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000194">
        <property type="entry name" value="ATPase_F1/V1/A1_a/bsu_nucl-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00006">
        <property type="entry name" value="ATP-synt_ab"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00306">
        <property type="entry name" value="ATP-synt_ab_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02874">
        <property type="entry name" value="ATP-synt_ab_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF039088">
        <property type="entry name" value="F_ATPase_subunit_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50615">
        <property type="entry name" value="SSF50615"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00962">
        <property type="entry name" value="atpA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00152">
        <property type="entry name" value="ATPASE_ALPHA_BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0066">ATP synthesis</keyword>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0997">Cell inner membrane</keyword>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0139">CF(1)</keyword>
    <keyword id="KW-0375">Hydrogen ion transport</keyword>
    <keyword id="KW-0406">Ion transport</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-1278">Translocase</keyword>
    <keyword id="KW-0813">Transport</keyword>
    <feature type="chain" id="PRO_0000256101" description="ATP synthase subunit alpha">
        <location>
            <begin position="1"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="169"/>
            <end position="176"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="site" description="Required for activity" evidence="1">
        <location>
            <position position="373"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01346"/>
        </source>
    </evidence>
    <sequence length="513" mass="55473" checksum="ABFA5C84619BEDF6" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="97" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LHS5</accession>
    <name>Q1LHS5_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Phosphoheptose isomerase</fullName>
            <ecNumber evidence="9">5.3.1.28</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Sedoheptulose 7-phosphate isomerase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">yraO</name>
        <name evidence="9" type="synonym">gmhA</name>
        <name evidence="11" type="ordered locus">Rmet_3429</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Catalyzes the isomerization of sedoheptulose 7-phosphate in D-glycero-D-manno-heptose 7-phosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 9">
            <text>2 D-sedoheptulose 7-phosphate = D-glycero-alpha-D-manno-heptose 7-phosphate + D-glycero-beta-D-manno-heptose 7-phosphate</text>
            <dbReference type="Rhea" id="RHEA:27489"/>
            <dbReference type="ChEBI" id="CHEBI:57483"/>
            <dbReference type="ChEBI" id="CHEBI:60203"/>
            <dbReference type="ChEBI" id="CHEBI:60204"/>
            <dbReference type="EC" id="5.3.1.28"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="9">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="9">Carbohydrate biosynthesis; D-glycero-D-manno-heptose 7-phosphate biosynthesis; D-glycero-alpha-D-manno-heptose 7-phosphate and D-glycero-beta-D-manno-heptose 7-phosphate from sedoheptulose 7-phosphate: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Homotetramer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 9">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="miscellaneous">
        <text evidence="9">The reaction produces a racemic mixture of D-glycero-alpha-D-manno-heptose 7-phosphate and D-glycero-beta-D-manno-heptose 7-phosphate.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the SIS family. GmhA subfamily.</text>
    </comment>
    <dbReference type="EC" id="5.3.1.28" evidence="9"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10301.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008646124.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3429"/>
    <dbReference type="EnsemblBacteria" id="ABF10301">
        <property type="protein sequence ID" value="ABF10301"/>
        <property type="gene ID" value="Rmet_3429"/>
    </dbReference>
    <dbReference type="GeneID" id="60822534"/>
    <dbReference type="KEGG" id="rme:Rmet_3429"/>
    <dbReference type="eggNOG" id="COG0279">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_080999_4_0_4"/>
    <dbReference type="OMA" id="DVHICVP"/>
    <dbReference type="OrthoDB" id="1571626at2"/>
    <dbReference type="UniPathway" id="UPA00041">
        <property type="reaction ID" value="UER00436"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097367">
        <property type="term" value="F:carbohydrate derivative binding"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008968">
        <property type="term" value="F:D-sedoheptulose 7-phosphate isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
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        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:2001061">
        <property type="term" value="P:D-glycero-D-manno-heptose 7-phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd05006">
        <property type="entry name" value="SIS_GmhA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00067">
        <property type="entry name" value="GmhA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035461">
        <property type="entry name" value="GmhA/DiaA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004515">
        <property type="entry name" value="Phosphoheptose_Isoase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001347">
        <property type="entry name" value="SIS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046348">
        <property type="entry name" value="SIS_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13580">
        <property type="entry name" value="SIS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53697">
        <property type="entry name" value="SSF53697"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00441">
        <property type="entry name" value="gmhA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51464">
        <property type="entry name" value="SIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 9" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="4 9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 9" id="KW-0413">Isomerase</keyword>
    <keyword evidence="5 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="SIS" evidence="10">
        <location>
            <begin position="35"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="9">
        <location>
            <begin position="50"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="9">
        <location>
            <begin position="92"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="9">
        <location>
            <begin position="118"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="59"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="63"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="123"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="173"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="181"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000348"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ABF10301.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="195" mass="20389" checksum="3437D73F5B2F1D26" modified="2006-05-30" version="1">MSIESIEQQFLDSAETKRMAATLMAPHIDAAVERMVAALTSGNKILACGNGGSAADAQHFAAELVGRFERERPGLAAIALTTDSSILTAIGNDYDFSVVFSKQVEALGQPGDILLALSTSGNSANVIAAIHAAHQRDMSVVALTGKGGGKIATVLDEFDIDLCVPSDRTARIQEVHLLTLHCLCDGIDEALLGEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="95" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LHW2</accession>
    <name>Q1LHW2_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="10">General secretory pathway component, cryptic</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">gspD</name>
        <name evidence="10" type="ordered locus">Rmet_3392</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cell outer membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the bacterial secretin family. GSP D subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10264.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3392"/>
    <dbReference type="EnsemblBacteria" id="ABF10264">
        <property type="protein sequence ID" value="ABF10264"/>
        <property type="gene ID" value="Rmet_3392"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3392"/>
    <dbReference type="eggNOG" id="COG1450">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG4796">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006756_1_1_4"/>
    <dbReference type="OMA" id="YAVVEMD"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009279">
        <property type="term" value="C:cell outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015627">
        <property type="term" value="C:type II protein secretion system complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015628">
        <property type="term" value="P:protein secretion by the type II secretion system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.120">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001775">
        <property type="entry name" value="GspD/PilQ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005644">
        <property type="entry name" value="NolW-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038591">
        <property type="entry name" value="NolW-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004846">
        <property type="entry name" value="T2SS/T3SS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013356">
        <property type="entry name" value="T2SS_GspD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00263">
        <property type="entry name" value="Secretin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03958">
        <property type="entry name" value="Secretin_N"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00811">
        <property type="entry name" value="BCTERIALGSPD"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02517">
        <property type="entry name" value="type_II_gspD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1134">Transmembrane beta strand</keyword>
    <keyword evidence="6" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193482" evidence="8">
        <location>
            <begin position="36"/>
            <end position="783"/>
        </location>
    </feature>
    <feature type="domain" description="Secretin_N" evidence="9">
        <location>
            <begin position="154"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="domain" description="Secretin_N" evidence="9">
        <location>
            <begin position="217"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="domain" description="Secretin_N" evidence="9">
        <location>
            <begin position="298"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="323"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="729"/>
            <end position="783"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="7">
        <location>
            <begin position="729"/>
            <end position="768"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="769"/>
            <end position="783"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU004004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF03958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ABF10264.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="783" mass="82207" checksum="3FA25B987B9EF115" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="114" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LIB0</accession>
    <name>Q1LIB0_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Imidazole glycerol phosphate synthase subunit HisH</fullName>
            <ecNumber evidence="9">4.3.2.10</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">IGP synthase glutaminase subunit</fullName>
            <ecNumber evidence="9">3.5.1.2</ecNumber>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">IGP synthase subunit HisH</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">ImGP synthase subunit HisH</fullName>
            <shortName evidence="9">IGPS subunit HisH</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9 12" type="primary">hisH</name>
        <name evidence="12" type="ordered locus">Rmet_3244</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The HisH subunit catalyzes the hydrolysis of glutamine to glutamate and ammonia as part of the synthesis of IGP and AICAR. The resulting ammonia molecule is channeled to the active site of HisF.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 9">
            <text>5-[(5-phospho-1-deoxy-D-ribulos-1-ylimino)methylamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + L-glutamine = 5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + D-erythro-1-(imidazol-4-yl)glycerol 3-phosphate + H(+) + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:24793"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:58278"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:58475"/>
            <dbReference type="ChEBI" id="CHEBI:58525"/>
            <dbReference type="EC" id="4.3.2.10"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2 9">
            <text>H2O + L-glutamine = L-glutamate + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:15889"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="EC" id="3.5.1.2"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3 9">Amino-acid biosynthesis; L-histidine biosynthesis; L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 5/9.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4 9">Heterodimer of HisH and HisF.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="4.3.2.10" evidence="9"/>
    <dbReference type="EC" id="3.5.1.2" evidence="9"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10116.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517720.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3244"/>
    <dbReference type="EnsemblBacteria" id="ABF10116">
        <property type="protein sequence ID" value="ABF10116"/>
        <property type="gene ID" value="Rmet_3244"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3244"/>
    <dbReference type="eggNOG" id="COG0118">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_071837_2_0_4"/>
    <dbReference type="OMA" id="WVYFVHS"/>
    <dbReference type="UniPathway" id="UPA00031">
        <property type="reaction ID" value="UER00010"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004359">
        <property type="term" value="F:glutaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000107">
        <property type="term" value="F:imidazoleglycerol-phosphate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006541">
        <property type="term" value="P:glutamine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000105">
        <property type="term" value="P:histidine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01748">
        <property type="entry name" value="GATase1_IGP_Synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00278">
        <property type="entry name" value="HisH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017926">
        <property type="entry name" value="GATASE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010139">
        <property type="entry name" value="Imidazole-glycPsynth_HisH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42701">
        <property type="entry name" value="PTHR42701"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00117">
        <property type="entry name" value="GATase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000495">
        <property type="entry name" value="Amidotransf_hisH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="SSF52317"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01855">
        <property type="entry name" value="IMP_synth_hisH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9 12" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="12" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="7 9" id="KW-0368">Histidine biosynthesis</keyword>
    <keyword evidence="6 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8 9" id="KW-0456">Lyase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="GATase" evidence="11">
        <location>
            <begin position="6"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="9 10">
        <location>
            <position position="82"/>
        </location>
    </feature>
    <feature type="active site" evidence="9 10">
        <location>
            <position position="197"/>
        </location>
    </feature>
    <feature type="active site" evidence="9 10">
        <location>
            <position position="199"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000619"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001062"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR000495-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ABF10116.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="217" mass="23805" checksum="A19E94E25E9AA323" modified="2006-05-30" version="1">MTTIAIVDYGMGNLRSVAQALRTAAPEADVQVVDRPEAIRAADRVVLPGQGAMPDCMGALGNSGLQEAVLEAAATKPMLGVCVGEQMLFERSSEARAGERSTPALGLMPGEVIRFELEGMTQPDGSRYKVPQMGWNRVRQSKPHPLWNGIPDNSWFYFVHSYYVHAQDPAHIAGETEYGVVFTSAVARDNIFATQFHPEKSAAMGLQLYRNFVHWNP</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LIQ8</accession>
    <name>Q1LIQ8_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Lysine exporter protein (LYSE/YGGA)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Rmet_3096</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09968.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517596.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF09968">
        <property type="protein sequence ID" value="ABF09968"/>
        <property type="gene ID" value="Rmet_3096"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3096"/>
    <dbReference type="eggNOG" id="COG1280">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_079569_1_0_4"/>
    <dbReference type="OMA" id="NPKAWIM"/>
    <dbReference type="OrthoDB" id="1945085at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006865">
        <property type="term" value="P:amino acid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001123">
        <property type="entry name" value="LeuE-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30086">
        <property type="entry name" value="PTHR30086"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01810">
        <property type="entry name" value="LysE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="54"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="85"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="150"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="189"/>
            <end position="209"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABF09968.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="212" mass="22526" checksum="184DC77687B30A1E" modified="2006-05-30" version="1">MTFPAFLPNVAAGSGVLLAFCAFALVSSITPGPNNTMVLASGVNFGIARTVPHLLGISIGFSVMVALVGLGLGSIFTAWPWMWDLLRVVATAYLVWLAWKLATAGGVQDREVARPMSFLRAAAFQWVNPKAWVMAVGACSTYVLDANIWVNAMAMACLFAVINLPSVATWAVFGAALRSLLARPRVLRVFNVTMALMLLASLLPILGAHPHG</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="57" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LIX7</accession>
    <name>Q1LIX7_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF2285 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_3026</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09899.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517541.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3026"/>
    <dbReference type="EnsemblBacteria" id="ABF09899">
        <property type="protein sequence ID" value="ABF09899"/>
        <property type="gene ID" value="Rmet_3026"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3026"/>
    <dbReference type="eggNOG" id="COG5419">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1080626_0_0_4"/>
    <dbReference type="OMA" id="HPAWFPD"/>
    <dbReference type="OrthoDB" id="1958544at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018754">
        <property type="entry name" value="DUF2285"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10074">
        <property type="entry name" value="DUF2285"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="15"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="domain" description="DUF2285" evidence="2">
        <location>
            <begin position="130"/>
            <end position="236"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF10074"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF09899.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="252" mass="27752" checksum="B44201CD0CEFBFCC" modified="2006-05-30" version="1">MAEPHHLAHWYPTAAYLYVLCLDTLALAWEYLRRHPDYRIDWLRRARCPDAAHRWGLRLLEDPDVDARDAHPAWLPGHGAVVQLHPDADPPPDATAFAFWRIPGHKQLLHDGKGLALIARSPSLCQRYALAPGLEDGMAVAHAYRGRHAAPAAPMPGTPASMARPRPPPAALLELHTLQALDATLAGASLRDVAEGLFGADAAAGWYSDGGLRSKVRRLVRRGDALMRGGYRRLAQLPPLEKGRFEESAKRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-04-07" version="57" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LJ06</accession>
    <name>Q1LJ06_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_2997</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09870.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517524.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF09870">
        <property type="protein sequence ID" value="ABF09870"/>
        <property type="gene ID" value="Rmet_2997"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2997"/>
    <dbReference type="eggNOG" id="COG1280">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1692619_0_0_4"/>
    <dbReference type="OrthoDB" id="1927576at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="39"/>
            <end position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF09870.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="155" mass="16180" checksum="4A9311D66B4625B7" modified="2006-05-30" version="1">MTPLDPLLLFVVANTVGPSTSSLYSLYLGATYGVRGALGYTLGTSLGFSIVVTTSSVLVAVAYEIPAGGQFGLKLFASGYTAWIASKCLRTAWTSSRDSTKALAFTKLGAYDGAIQQLANPRGWALATVSVVGFSDAQVPPLMEGSSQKTENKAR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-01-15" modified="2022-05-25" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LJ60</accession>
    <name>QUEA_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">S-adenosylmethionine:tRNA ribosyltransferase-isomerase</fullName>
            <ecNumber evidence="1">2.4.99.17</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Queuosine biosynthesis protein QueA</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">queA</name>
        <name type="ordered locus">Rmet_2943</name>
    </gene>
    <organism>
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Transfers and isomerizes the ribose moiety from AdoMet to the 7-aminomethyl group of 7-deazaguanine (preQ1-tRNA) to give epoxyqueuosine (oQ-tRNA).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>7-aminomethyl-7-carbaguanosine(34) in tRNA + S-adenosyl-L-methionine = adenine + epoxyqueuosine(34) in tRNA + H(+) + L-methionine</text>
            <dbReference type="Rhea" id="RHEA:32155"/>
            <dbReference type="Rhea" id="RHEA-COMP:10342"/>
            <dbReference type="Rhea" id="RHEA-COMP:10346"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16708"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:82833"/>
            <dbReference type="ChEBI" id="CHEBI:82834"/>
            <dbReference type="EC" id="2.4.99.17"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">tRNA modification; tRNA-queuosine biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the QueA family.</text>
    </comment>
    <dbReference type="EC" id="2.4.99.17" evidence="1"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09816.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517483.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q1LJ60"/>
    <dbReference type="SMR" id="Q1LJ60"/>
    <dbReference type="STRING" id="266264.Rmet_2943"/>
    <dbReference type="EnsemblBacteria" id="ABF09816">
        <property type="protein sequence ID" value="ABF09816"/>
        <property type="gene ID" value="Rmet_2943"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2943"/>
    <dbReference type="eggNOG" id="COG0809">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039110_1_0_4"/>
    <dbReference type="OMA" id="YSYGDGM"/>
    <dbReference type="OrthoDB" id="368001at2"/>
    <dbReference type="UniPathway" id="UPA00392"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051075">
        <property type="term" value="F:S-adenosylmethionine:tRNA ribosyltransferase-isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008616">
        <property type="term" value="P:queuosine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.240">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1780.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00113">
        <property type="entry name" value="QueA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003699">
        <property type="entry name" value="QueA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042118">
        <property type="entry name" value="QueA_dom1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042119">
        <property type="entry name" value="QueA_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036100">
        <property type="entry name" value="QueA_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30307">
        <property type="entry name" value="PTHR30307"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02547">
        <property type="entry name" value="Queuosine_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111337">
        <property type="entry name" value="SSF111337"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00113">
        <property type="entry name" value="queA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0671">Queuosine biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_1000015253" description="S-adenosylmethionine:tRNA ribosyltransferase-isomerase">
        <location>
            <begin position="1"/>
            <end position="353"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00113"/>
        </source>
    </evidence>
    <sequence length="353" mass="39035" checksum="7BE01ADB508736C7" modified="2006-05-30" version="1">MLTLSDFDFPLPPELIAQSALPDRSASRLLVVERTAPEDTSEAVRLVDRAFSDILEYLNPDDLLVFNDTRVIKARFFGHKPSGGRIEVLVERVVDTHTVLAQVRASKTPVEGSLLHLADDAFAVTVGPRVDQFFTLRFPEPALDLIERYGRLPLPPYITHDPDAYDETRYQTVYARNPGAVAAPTAGLHFDDALFARLDAAGIRRAFLTLHVGAGTFQPVRTENLSEHKMHSEWYAISPELADAVRETRARGGRVIAVGTTSLRALESAAAEDGTLEAGSGDTDIFITPGYAFRIVDALITNFHLPKSTLLMLVSALAGVEAIRDAYRHAVDARYRFFSYGDAMLLTRRDRRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="101" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LJE7</accession>
    <name>Q1LJE7_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Histidine utilization repressor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">hutC</name>
        <name evidence="4" type="ordered locus">Rmet_2856</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09729.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517414.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2856"/>
    <dbReference type="EnsemblBacteria" id="ABF09729">
        <property type="protein sequence ID" value="ABF09729"/>
        <property type="gene ID" value="Rmet_2856"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2856"/>
    <dbReference type="eggNOG" id="COG2188">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_063236_0_0_4"/>
    <dbReference type="OMA" id="LLNQVPW"/>
    <dbReference type="OrthoDB" id="1775418at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006547">
        <property type="term" value="P:histidine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045892">
        <property type="term" value="P:negative regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006744">
        <property type="term" value="P:ubiquinone biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd07377">
        <property type="entry name" value="WHTH_GntR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1410.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028978">
        <property type="entry name" value="Chorismate_lyase_/UTRA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010248">
        <property type="entry name" value="His_ut_repres"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000524">
        <property type="entry name" value="Tscrpt_reg_HTH_GntR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011663">
        <property type="entry name" value="UTRA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00392">
        <property type="entry name" value="GntR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07702">
        <property type="entry name" value="UTRA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00035">
        <property type="entry name" value="HTHGNTR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00345">
        <property type="entry name" value="HTH_GNTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00866">
        <property type="entry name" value="UTRA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64288">
        <property type="entry name" value="SSF64288"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02018">
        <property type="entry name" value="his_ut_repres"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50949">
        <property type="entry name" value="HTH_GNTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0456">Lyase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0831">Ubiquinone biosynthesis</keyword>
    <feature type="domain" description="HTH gntR-type" evidence="3">
        <location>
            <begin position="18"/>
            <end position="86"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF09729.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="255" mass="28359" checksum="407BA786C14E8830" modified="2006-05-30" version="1">MNQSASQAPTPTNATAPAALYRQVKEYIARQIQSGVWKPGDRVPSEQELVNRFSVSRMTVNRALRELSEQGRVVRVAGVGTFVAEQKPQSTLLSVVNLQDEIRMRGHDYACDVILVERVSAPIEVAAALELHTGESVYHSVCVHREDGVPVQLEDRYVNPRVAPDFINQDFSGTKPGEYLLRNVPYDQVEHVVDAISATPEQAAQLEMPPTQPCLLLTRRTWTNGVPVTFVRCLHPGNRYRLGSRFRADGNPAFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LJH3</accession>
    <name>Q1LJH3_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">AAA_23 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_2830</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09703.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517397.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2830"/>
    <dbReference type="EnsemblBacteria" id="ABF09703">
        <property type="protein sequence ID" value="ABF09703"/>
        <property type="gene ID" value="Rmet_2830"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2830"/>
    <dbReference type="eggNOG" id="COG1196">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026561_0_0_4"/>
    <dbReference type="OMA" id="NATIACK"/>
    <dbReference type="OrthoDB" id="1565092at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140664">
        <property type="term" value="F:ATP-dependent DNA damage sensor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038729">
        <property type="entry name" value="Rad50/SbcC_AAA"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13476">
        <property type="entry name" value="AAA_23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA_23" evidence="2">
        <location>
            <begin position="9"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="404"/>
            <end position="459"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13476"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF09703.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="676" mass="75146" checksum="7FF2ACF22A3B0967" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LJT2</accession>
    <name>Q1LJT2_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative glycosyltransferase, group 1 (Probably involved in lipopolysaccharide biosynthesis)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_2721</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09594.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517293.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2721"/>
    <dbReference type="CAZy" id="GT4">
        <property type="family name" value="Glycosyltransferase Family 4"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF09594">
        <property type="protein sequence ID" value="ABF09594"/>
        <property type="gene ID" value="Rmet_2721"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2721"/>
    <dbReference type="eggNOG" id="COG0438">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009583_0_0_4"/>
    <dbReference type="OMA" id="WADLHLV"/>
    <dbReference type="OrthoDB" id="635734at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001296">
        <property type="entry name" value="Glyco_trans_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028098">
        <property type="entry name" value="Glyco_trans_4-like_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13439">
        <property type="entry name" value="Glyco_transf_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00534">
        <property type="entry name" value="Glycos_transf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glyco_trans_4-like_N" evidence="2">
        <location>
            <begin position="20"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="domain" description="Glycos_transf_1" evidence="1">
        <location>
            <begin position="179"/>
            <end position="340"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13439"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF09594.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="364" mass="40111" checksum="28D344381B53A5BE" modified="2006-05-30" version="1">MSTPLPTVCFLTGTLNAFAGAERMTAVIANALAERGYRVLVLSLWDRTSVFPLHEGVEHHALFEQRPSFKRAYVSTVLGIRRFVREQGIDVLIEVDTMLTLFTLPATSGMKVRRIAWEHCHFDEDLGRKARRVARWLAAKTSSAVVVLTERDRQRWLNALGQHCRIATIGNPLPFAFPAEPAQRGSRIVLAVGRLVRAKGFDVLLDAWARVAPAHPDWRLVIVGEGEERDALEAQIRSLALGHCVDLPGASSEIASYYGEASIFCLSSRYEGFGLVLMEAMAFGLPVVSTNCEAGPKALIRDGVNGLQVPAEDALALGAALSRLMQEKHLCEALSSRARDDARRYEIDAICPLWQNLLGDIQTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="70" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LJT3</accession>
    <name>Q1LJT3_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative lipopolysaccharide biosynthesis protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_2720</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09593.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517292.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2720"/>
    <dbReference type="EnsemblBacteria" id="ABF09593">
        <property type="protein sequence ID" value="ABF09593"/>
        <property type="gene ID" value="Rmet_2720"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2720"/>
    <dbReference type="eggNOG" id="COG2244">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_054018_0_0_4"/>
    <dbReference type="OMA" id="IYYRTDM"/>
    <dbReference type="OrthoDB" id="564091at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="48"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="81"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="117"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="147"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="174"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="207"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="248"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="288"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="326"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="362"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="386"/>
            <end position="405"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF09593.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="416" mass="44146" checksum="E363CAE0EB8BD0D5" modified="2006-05-30" version="1">MRRRVAGNLLWMLAERGFQIGIGIAVVAMLARALGPAGFAHFQYAQSIVLIAASFALVCGAEVVVPRLVASPAPATQHALLAHAFVLRLAGGAIGYLLMCAYLGITSPGPEVWHAALWLGIAILLREPFGVVTAWMQSHTNNRPGTLFSLAALAVKAILVGSLFAAGVHKITGYAAAFAIEAIILAVLLTSYYFVRVHDRDFAWQATQARELITSGALFWISFILMMGARRVDQLVLQPAVPPADFGAYAACMQILDNFTMVASILVAGIAPAYVYAKAAYAEAHANIGRIALGIAALGLVGGMAIAGCAPWIVRLLYGNAFDATVTLLRVAAVASTLVFADVALTLLAVYLRRPRWVATKWALVFGATLAFDLIVIPRYGSWGAIAGYALGNGVAVLVGLTLWWRHRPAPEVASI</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LJU2</accession>
    <name>Q1LJU2_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Type I secretion outer membrane efflux protein (TolC-like)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Rmet_2711</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the outer membrane factor (OMF) (TC 1.B.17) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09584.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517283.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2711"/>
    <dbReference type="EnsemblBacteria" id="ABF09584">
        <property type="protein sequence ID" value="ABF09584"/>
        <property type="gene ID" value="Rmet_2711"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2711"/>
    <dbReference type="eggNOG" id="COG1538">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012817_0_2_4"/>
    <dbReference type="OMA" id="YNAKQQL"/>
    <dbReference type="OrthoDB" id="1099555at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019867">
        <property type="term" value="C:outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015562">
        <property type="term" value="F:efflux transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003423">
        <property type="entry name" value="OMP_efflux"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010130">
        <property type="entry name" value="T1SS_OMP_TolC"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02321">
        <property type="entry name" value="OEP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01844">
        <property type="entry name" value="type_I_sec_TolC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193169" evidence="4">
        <location>
            <begin position="34"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="466"/>
            <end position="493"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABF09584.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="493" mass="52910" checksum="8388502AF63BD7B3" modified="2006-05-30" version="1">MSFPLSAVPGAARRSLPLAAALVAMLHLPAAESADLLQVYRDAQSNDAQFASARAQLVATREKLPQARSGLLPQVFGTAGAQRTQADYSSPVDVTRYFNANTWQLQLTQPLFRWDRWETYKQGELAALAGEITFQQAQLDLITRASQAYFDVLAAQDNLYLAGAQKKAIGEQLEQAKRNFEVGTATITDANDAQARYDLATSTEIASQSALEVSRATLQQITGKSVDELMGLRSEAAIPGPAPADVNAWAAQAEQANPQVSLAGYNLETAQRETNKAKAGHLPSVDLVASYGYTNAQGTATQLSNVGSRYNSGVVGVQLSIPIFTGGQIQSRVRETLALADKAASDLEFARRTAAQTARQTYSGVYNGLAQVKALEAAERSAQSAVESNQLGYEVGVRINIDVLNAEAQLFQTRRDLAKARYDTIMNGMRLKASTGALSEDDVVQINTLLTSAPGAMYKLPGKTRVGAMPAHKPARGERGRRESMAQGVTPRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LK27</accession>
    <name>Q1LK27_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative carboxymethylenebutenolidase (Dienelactone hydrolase)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_2622</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09499.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517200.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2622"/>
    <dbReference type="ESTHER" id="ralme-q1lk27">
        <property type="family name" value="Dienelactone_hydrolase"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF09499">
        <property type="protein sequence ID" value="ABF09499"/>
        <property type="gene ID" value="Rmet_2622"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2622"/>
    <dbReference type="eggNOG" id="COG0412">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_054590_7_0_4"/>
    <dbReference type="OMA" id="HIKAYVV"/>
    <dbReference type="OrthoDB" id="1889569at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002925">
        <property type="entry name" value="Dienelactn_hydro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006311">
        <property type="entry name" value="TAT_signal"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01738">
        <property type="entry name" value="DLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="SSF53474"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51318">
        <property type="entry name" value="TAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DLH" evidence="1">
        <location>
            <begin position="63"/>
            <end position="288"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF09499.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="291" mass="31550" checksum="7EE9B4DC7AEB99F8" modified="2006-05-30" version="1">MLKPEVESLVPGQTFDRRSFVKTALGSAFAAAVLPVHATTIKTDFKGLTAGEVTVESNGFKMPVYRAQPEGKTNLPVVLVISEIFGVHEHIADICRRFAKLGYLAIAPELFARQGDPSSYGTIQELQANIIQKVPDAQVMGDLDACVTWAGANGGDLSRLAITGFCWGGRITWLYTAHNNRVKAGVAWYGQLVGEPTALKPRNSVDIVNELDAPVLGLYGGKDTGITQEQVERMKAALAASSNPNAKASRFVVYPEAGHAFNADYRASYVESAAKDGWQRCLDWLRQHKVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LKF3</accession>
    <name>Q1LKF3_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative extracellular solute-binding, family 5 ABC-type transporter protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_2496</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09373.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2496"/>
    <dbReference type="EnsemblBacteria" id="ABF09373">
        <property type="protein sequence ID" value="ABF09373"/>
        <property type="gene ID" value="Rmet_2496"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2496"/>
    <dbReference type="eggNOG" id="COG0747">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017028_7_4_4"/>
    <dbReference type="OMA" id="YIPLTYQ"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043190">
        <property type="term" value="C:ATP-binding cassette (ABC) transporter complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030288">
        <property type="term" value="C:outer membrane-bounded periplasmic space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030678">
        <property type="entry name" value="Peptide/Ni-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039424">
        <property type="entry name" value="SBP_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000914">
        <property type="entry name" value="SBP_5_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006311">
        <property type="entry name" value="TAT_signal"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30290">
        <property type="entry name" value="PTHR30290"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00496">
        <property type="entry name" value="SBP_bac_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002741">
        <property type="entry name" value="MppA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51318">
        <property type="entry name" value="TAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193177" evidence="1">
        <location>
            <begin position="41"/>
            <end position="538"/>
        </location>
    </feature>
    <feature type="domain" description="SBP_bac_5" evidence="2">
        <location>
            <begin position="84"/>
            <end position="441"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF09373.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="538" mass="59162" checksum="693510F519FE7EF0" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LKS6</accession>
    <name>Q1LKS6_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_2373</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09250.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2373"/>
    <dbReference type="EnsemblBacteria" id="ABF09250">
        <property type="protein sequence ID" value="ABF09250"/>
        <property type="gene ID" value="Rmet_2373"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2373"/>
    <dbReference type="eggNOG" id="COG0446">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG3453">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030742_2_2_4"/>
    <dbReference type="OMA" id="YKPAFMY"/>
    <dbReference type="OrthoDB" id="403436at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="match status" value="2"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="BLH_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
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        <property type="entry name" value="FAD/NAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015904">
        <property type="entry name" value="Sulphide_quinone_reductase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10632">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07992">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01244">
        <property type="entry name" value="TIGR01244"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF442" evidence="1">
        <location>
            <begin position="2"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="domain" description="Pyr_redox_2" evidence="2">
        <location>
            <begin position="150"/>
            <end position="461"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF04273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF09250.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="556" mass="59638" checksum="3384DF5B9DB6928C" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LKU9</accession>
    <name>Q1LKU9_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_2350</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09227.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2350"/>
    <dbReference type="EnsemblBacteria" id="ABF09227">
        <property type="protein sequence ID" value="ABF09227"/>
        <property type="gene ID" value="Rmet_2350"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2350"/>
    <dbReference type="eggNOG" id="COG1450">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039053_1_0_4"/>
    <dbReference type="OMA" id="WRLGDYW"/>
    <dbReference type="OrthoDB" id="775038at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021844">
        <property type="entry name" value="Integr_conj_element_PFL4704"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11920">
        <property type="entry name" value="DUF3438"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03749">
        <property type="entry name" value="conj_TIGR03749"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193638" evidence="2">
        <location>
            <begin position="23"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="121"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="284"/>
            <end position="303"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF09227.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="303" mass="32649" checksum="49B7E5F37B3675EA" modified="2006-05-30" version="1">MKHPVLALLGLLAVAAAPVAQAVEILRWERMPLAVPLKVGQERIVFIDRNVRVGVPAGVGERLRVQSAGGAVYLRASEPIEPTRLQLQDADTGALILLDIAAEPPKDGEAELEPVRIVEGDSAPGRYGEQADSAEAPARAQGQAGARTARRETPVPVVLTRFAAQNLYAPLRTVEPLPGVMRVNLPRDLDLDTLMPTLPVRAVALASWRLEDQWVTAVRLTNTGADWVALDPRVLQGDFLTATFQHEALGPRGTPEDTTVLYLVTGGRGLAQSLLPAIHRFDPAVHLPQPDGDENAKEARHAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="95" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LKY5</accession>
    <name>Q1LKY5_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Mercuric resistance operon regulatory protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">merR</name>
        <name evidence="11" type="ordered locus">Rmet_2312</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Mediates the mercuric-dependent induction of mercury resistance operon. In the absence of mercury MerR represses transcription by binding tightly to the mer operator region; when mercury is present the dimeric complex binds a single ion and becomes a potent transcriptional activator, while remaining bound to the mer site.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09191.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_003098939.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2312"/>
    <dbReference type="EnsemblBacteria" id="ABF09191">
        <property type="protein sequence ID" value="ABF09191"/>
        <property type="gene ID" value="Rmet_2312"/>
    </dbReference>
    <dbReference type="GeneID" id="56409820"/>
    <dbReference type="GeneID" id="61526438"/>
    <dbReference type="KEGG" id="rme:Rmet_2312"/>
    <dbReference type="eggNOG" id="COG0789">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_060077_2_0_4"/>
    <dbReference type="OMA" id="DCAVETI"/>
    <dbReference type="OrthoDB" id="1748559at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045340">
        <property type="term" value="F:mercury ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046689">
        <property type="term" value="P:response to mercury ion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04783">
        <property type="entry name" value="HTH_MerR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009061">
        <property type="entry name" value="DNA-bd_dom_put_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011794">
        <property type="entry name" value="MerR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000551">
        <property type="entry name" value="MerR-type_HTH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015358">
        <property type="entry name" value="Tscrpt_reg_MerR_DNA-bd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00376">
        <property type="entry name" value="MerR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09278">
        <property type="entry name" value="MerR-DNA-bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00040">
        <property type="entry name" value="HTHMERR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00422">
        <property type="entry name" value="HTH_MERR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46955">
        <property type="entry name" value="SSF46955"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02051">
        <property type="entry name" value="MerR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00552">
        <property type="entry name" value="HTH_MERR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50937">
        <property type="entry name" value="HTH_MERR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0010">Activator</keyword>
    <keyword evidence="6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0475">Mercuric resistance</keyword>
    <keyword evidence="4" id="KW-0476">Mercury</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0678">Repressor</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH merR-type" evidence="10">
        <location>
            <begin position="7"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00017146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00024874"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ABF09191.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="135" mass="14989" checksum="ECF24130712CE782" modified="2006-05-30" version="1">MENAQENLTIGAFAKAARVNVETIRFYQLKGLLPQPERPYGRIRRYGQADVARVKFVKSAQRLGFSLDEVGQLLKLEDGTHCSEAAELAAHRLADVRARMADLTRMEEALSTLVRECNAHHGNVSCPLIAALHCC</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LLF8</accession>
    <name>Q1LLF8_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Na+ driven multidrug efflux pump (MATE family)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">mdtK</name>
        <name evidence="2" type="ordered locus">Rmet_2139</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09018.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516847.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2139"/>
    <dbReference type="EnsemblBacteria" id="ABF09018">
        <property type="protein sequence ID" value="ABF09018"/>
        <property type="gene ID" value="Rmet_2139"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2139"/>
    <dbReference type="eggNOG" id="COG0534">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012893_6_0_4"/>
    <dbReference type="OMA" id="EFWILLK"/>
    <dbReference type="OrthoDB" id="1415498at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015297">
        <property type="term" value="F:antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042910">
        <property type="term" value="F:xenobiotic transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002528">
        <property type="entry name" value="MATE_fam"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01554">
        <property type="entry name" value="MatE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006603">
        <property type="entry name" value="DinF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00797">
        <property type="entry name" value="matE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="51"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="91"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="128"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="159"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="198"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="237"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="275"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="318"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="356"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="389"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="422"/>
            <end position="443"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF09018.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="449" mass="47890" checksum="43B34B9E7D3349CC" modified="2006-05-30" version="1">MSSALQDIRRITHLAAPVLVGQLAVIAFGVIDTIMSGRASATDLAAVGLGGSIYVTVYVSLMGVLQALAPVAGQLYGANRSAEIGAEVRQAAWLGVALAIPGMLLLLFPAPLLEFAKAPPELVEKASHYLHIGAYGLPAALGFRIYMALNNALSRPVMVTVLQIAGLALKIPLNAWFIEGGYGLAPMGGPGCALASTLISWIWFLSGLAILLRGAAYRSLEIFTRWEWPRWDRIQPLLRLGLPMGLTYLIEITSFTLMSIFITRMGTDTLAGHQIIANLSAVAYMMPLSLSIATSTLVAQSIGARDMAAARRIGWRGIRLGAGLALLTGTLLWLLREPLLHAYVSDPAVIAKALPLMLFVAFYQAFDAVQVMSAFILRAYKIALIPTLIYAGSLWGVGLGGGYVMGFGLIDALPAFTRGASGFWLANSISLAIAGTLLVRYFMRVSRVG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-10-31" modified="2022-05-25" version="72" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LLG0</accession>
    <name>RL31B_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">50S ribosomal protein L31 type B</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpmE2</name>
        <name type="ordered locus">Rmet_2137</name>
    </gene>
    <organism>
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="1">Part of the 50S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the bacterial ribosomal protein bL31 family. Type B subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09016.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008647952.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q1LLG0"/>
    <dbReference type="STRING" id="266264.Rmet_2137"/>
    <dbReference type="EnsemblBacteria" id="ABF09016">
        <property type="protein sequence ID" value="ABF09016"/>
        <property type="gene ID" value="Rmet_2137"/>
    </dbReference>
    <dbReference type="GeneID" id="60821281"/>
    <dbReference type="KEGG" id="rme:Rmet_2137"/>
    <dbReference type="eggNOG" id="COG0254">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_114306_2_1_4"/>
    <dbReference type="OMA" id="MQQDKHP"/>
    <dbReference type="OrthoDB" id="2014569at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.830.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00502">
        <property type="entry name" value="Ribosomal_L31_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034704">
        <property type="entry name" value="L28p-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002150">
        <property type="entry name" value="Ribosomal_L31"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027493">
        <property type="entry name" value="Ribosomal_L31_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042105">
        <property type="entry name" value="Ribosomal_L31_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33280">
        <property type="entry name" value="PTHR33280"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01197">
        <property type="entry name" value="Ribosomal_L31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01249">
        <property type="entry name" value="RIBOSOMALL31"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143800">
        <property type="entry name" value="SSF143800"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00105">
        <property type="entry name" value="L31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01143">
        <property type="entry name" value="RIBOSOMAL_L31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <feature type="chain" id="PRO_0000259117" description="50S ribosomal protein L31 type B">
        <location>
            <begin position="1"/>
            <end position="86"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00502"/>
        </source>
    </evidence>
    <sequence length="86" mass="9863" checksum="C037310D69FD534B" modified="2006-05-30" version="1">MKEGIHPNYREVLFHDVSNDFKFVTRSTIQTKDTIEHEGKTYPLAKIEVSSESHPFYTGTQKIMDTAGRVEKFRQKFGSKLGAAKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LLH8</accession>
    <name>Q1LLH8_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">NADPH:quinone oxidoreductase</fullName>
            <ecNumber evidence="2">1.6.5.5</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_2119</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EC" id="1.6.5.5" evidence="2"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08998.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2119"/>
    <dbReference type="EnsemblBacteria" id="ABF08998">
        <property type="protein sequence ID" value="ABF08998"/>
        <property type="gene ID" value="Rmet_2119"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2119"/>
    <dbReference type="eggNOG" id="COG0604">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026673_3_1_4"/>
    <dbReference type="OMA" id="FWGEFAR"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003960">
        <property type="term" value="F:NADPH:quinone reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013149">
        <property type="entry name" value="ADH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013154">
        <property type="entry name" value="ADH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020843">
        <property type="entry name" value="PKS_ER"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08240">
        <property type="entry name" value="ADH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00107">
        <property type="entry name" value="ADH_zinc_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00829">
        <property type="entry name" value="PKS_ER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="SSF50129"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PKS_ER" evidence="1">
        <location>
            <begin position="47"/>
            <end position="359"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF08998.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="361" mass="38275" checksum="74B49160C939991B" modified="2006-05-30" version="1">MDVMQRFFEQNDRAFPALHLAAAHAYPRACPIPTGDTMKAVLCKAWGPPDSLTLETLPDLVPGDGEVVIDVKAAGVNFPDVLIIQNKYQAKPELPFSPGSELAGVVNAVGAGVTHVKPRDKVIAYLGNGAFASQAKAPAKAVVPMPPGIDFDVAAAFTLTYGTSHHAVVDRGELKAGETMLVLGAAGGVGLAAIEIGKAIGARVIAAASTDEKLAVCKEHGADALINYSTEDLRERVKALTDGKGPDVIYDPVGGVYAEPAFRSIAWRGRYLVVGFANGEIPKIPLNLALLKGASLVGVFWGEFARREPKANQANMAQMLGWMKEGKIRPHISARYPLEQAPQALKDMEARKVTGKIVITP</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="102" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LLQ6</accession>
    <name>Q1LLQ6_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Cbb3-type cytochrome c oxidase subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="primary">ccoP</name>
        <name evidence="20" type="ordered locus">Rmet_2041</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="15">C-type cytochrome. Part of the cbb3-type cytochrome c oxidase complex.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="15 17">
            <name>heme c</name>
            <dbReference type="ChEBI" id="CHEBI:61717"/>
        </cofactor>
        <text evidence="15 17">Binds 2 heme C groups per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1 15">Energy metabolism; oxidative phosphorylation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="15">Component of the cbb3-type cytochrome c oxidase.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="15">Cell inner membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 15">Belongs to the CcoP / FixP family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08920.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516754.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2041"/>
    <dbReference type="EnsemblBacteria" id="ABF08920">
        <property type="protein sequence ID" value="ABF08920"/>
        <property type="gene ID" value="Rmet_2041"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2041"/>
    <dbReference type="eggNOG" id="COG2010">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047545_2_0_4"/>
    <dbReference type="OMA" id="DWLYGGE"/>
    <dbReference type="OrthoDB" id="1315115at2"/>
    <dbReference type="UniPathway" id="UPA00705"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070469">
        <property type="term" value="C:respirasome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006811">
        <property type="term" value="P:ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006119">
        <property type="term" value="P:oxidative phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.760.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.280.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032858">
        <property type="entry name" value="CcoP_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038414">
        <property type="entry name" value="CcoP_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009056">
        <property type="entry name" value="Cyt_c-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036909">
        <property type="entry name" value="Cyt_c-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004678">
        <property type="entry name" value="Cyt_c_oxidase_cbb3_su3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13442">
        <property type="entry name" value="Cytochrome_CBB3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14715">
        <property type="entry name" value="FixP_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000006">
        <property type="entry name" value="Cbb3-Cox_fixP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46626">
        <property type="entry name" value="SSF46626"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00782">
        <property type="entry name" value="ccoP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51007">
        <property type="entry name" value="CYTC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 15" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="3 15" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="15" id="KW-0249">Electron transport</keyword>
    <keyword evidence="5 15 17" id="KW-0349">Heme</keyword>
    <keyword evidence="9 15" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="13 15" id="KW-0406">Ion transport</keyword>
    <keyword evidence="12 15 16" id="KW-0408">Iron</keyword>
    <keyword evidence="14 15 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 15 16" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11 15" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="15" id="KW-0679">Respiratory chain</keyword>
    <keyword evidence="6 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 18" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="15" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="9"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="62"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="domain" description="Cytochrome c" evidence="19">
        <location>
            <begin position="130"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="domain" description="Cytochrome c" evidence="19">
        <location>
            <begin position="216"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="binding site" description="covalent" evidence="17">
        <location>
            <position position="143"/>
        </location>
        <ligand>
            <name>heme c</name>
            <dbReference type="ChEBI" id="CHEBI:61717"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" description="covalent" evidence="17">
        <location>
            <position position="146"/>
        </location>
        <ligand>
            <name>heme c</name>
            <dbReference type="ChEBI" id="CHEBI:61717"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="16">
        <location>
            <position position="147"/>
        </location>
        <ligand>
            <name>heme c</name>
            <dbReference type="ChEBI" id="CHEBI:61717"/>
            <label>1</label>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="16">
        <location>
            <position position="186"/>
        </location>
        <ligand>
            <name>heme c</name>
            <dbReference type="ChEBI" id="CHEBI:61717"/>
            <label>2</label>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <feature type="binding site" description="covalent" evidence="17">
        <location>
            <position position="229"/>
        </location>
        <ligand>
            <name>heme c</name>
            <dbReference type="ChEBI" id="CHEBI:61717"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" description="covalent" evidence="17">
        <location>
            <position position="232"/>
        </location>
        <ligand>
            <name>heme c</name>
            <dbReference type="ChEBI" id="CHEBI:61717"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="16">
        <location>
            <position position="233"/>
        </location>
        <ligand>
            <name>heme c</name>
            <dbReference type="ChEBI" id="CHEBI:61717"/>
            <label>2</label>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="16">
        <location>
            <position position="274"/>
        </location>
        <ligand>
            <name>heme c</name>
            <dbReference type="ChEBI" id="CHEBI:61717"/>
            <label>1</label>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRNR" id="PIRNR000006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR000006-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRSR" id="PIRSR000006-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS51007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="ABF08920.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="304" mass="33401" checksum="9C0F98FD0888D395" modified="2006-05-30" version="1">MSDFISDFWSYYIAAIALIGIVWCVWLLFSQRKMPKGVSTTDDTGHVWDGDLRELNNPLPRWWMWMFLLACIFGLGYLALYPGLGSYAGMLKFSTQGELAAHREAAEKTQHQIYAKYLNMDIKQVAADPDAREIGQRLFLNYCAQCHGSDARGSRGFPNLTDNDWLYGGEPETIQQTITKGRNGVMPSFASTIDAKQAGDTAQYVRSLSGLSSDPIRASRGESTFKSTCAACHGANGKGNQALGAPNLTDNTWLYGSSEATIVDAILKGHNGHMPAHEEILTPERIRMLTAYVWGLSNTGGAAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="50" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LLW4</accession>
    <name>Q1LLW4_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_1983</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08862.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516703.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF08862">
        <property type="protein sequence ID" value="ABF08862"/>
        <property type="gene ID" value="Rmet_1983"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1983"/>
    <dbReference type="HOGENOM" id="CLU_2551754_0_0_4"/>
    <dbReference type="OrthoDB" id="1859397at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF08862.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="90" mass="9583" checksum="799F2E0AE2C0EE4F" modified="2006-05-30" version="1">MTLRFHELSPDVREALESICARHGFVPDDFEFAVANVLNPGESAADMVTVERVVGGQLQKYKGGAGGAATWLAAFEADLASDWFGAPLAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LMD3</accession>
    <name>Q1LMD3_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Acyl-CoA dehydrogenase, short-chain specific</fullName>
            <ecNumber evidence="8">1.3.99.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Rmet_1814</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the acyl-CoA dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.3.99.-" evidence="8"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08693.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1814"/>
    <dbReference type="EnsemblBacteria" id="ABF08693">
        <property type="protein sequence ID" value="ABF08693"/>
        <property type="gene ID" value="Rmet_1814"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1814"/>
    <dbReference type="eggNOG" id="COG1960">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018204_1_0_4"/>
    <dbReference type="OMA" id="RMTHVMR"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016627">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-CH group of donors"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.540.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.110.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006091">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046373">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036250">
        <property type="entry name" value="AcylCo_DH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009075">
        <property type="entry name" value="AcylCo_DH/oxidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013786">
        <property type="entry name" value="AcylCoA_DH/ox_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037069">
        <property type="entry name" value="AcylCoA_DH/ox_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009100">
        <property type="entry name" value="AcylCoA_DH/oxidase_NM_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00441">
        <property type="entry name" value="Acyl-CoA_dh_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02770">
        <property type="entry name" value="Acyl-CoA_dh_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02771">
        <property type="entry name" value="Acyl-CoA_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47203">
        <property type="entry name" value="SSF47203"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56645">
        <property type="entry name" value="SSF56645"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0274">FAD</keyword>
    <keyword evidence="3" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Acyl-CoA_dh_N" evidence="7">
        <location>
            <begin position="10"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA_dh_M" evidence="6">
        <location>
            <begin position="127"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA_dh_1" evidence="5">
        <location>
            <begin position="236"/>
            <end position="384"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF02770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF02771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ABF08693.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="391" mass="42896" checksum="5F5E11FD0CE42BF6" modified="2006-05-30" version="1">MMAMGTNIAELRGKVRQFVEQHAIPSEDPVLAHDVQRLDVVVRRLQGLAREAGIYAPHLPPEHGGLGLDWQARAEILEEAGRSFLGAPALNCAPPDQPNMINLMRHGNAAQQARYLQPLVQGRIRSCFAMTEPAPGAGSDPSMLRTTATRQDGKWVINGHKWFISGAVDAAFAIVLARTDEAAGTGATMFIVDTDNPGYRLVRNIAGMDGYQVGGHGEIVFEQCVVDDDAVLGQVGQGFAYAQARLEPARLSHCMRFIGRASRAMEIAQQYAAQRESFGQRLADLQQVQAMVADSHIDLHASRLMVRDCARKMDAGESVKHDSAMTKIFVSEAVWRVADRAVQIMGALGISDDTPVSMIQRELRPFRIYDGASELHRATLARRIFQHGLRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="58" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LMK2</accession>
    <name>Q1LMK2_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF2726 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_1745</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08624.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1745"/>
    <dbReference type="EnsemblBacteria" id="ABF08624">
        <property type="protein sequence ID" value="ABF08624"/>
        <property type="gene ID" value="Rmet_1745"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1745"/>
    <dbReference type="eggNOG" id="COG0551">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_082936_3_1_4"/>
    <dbReference type="OMA" id="ELAIWPF"/>
    <dbReference type="OrthoDB" id="1999861at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024402">
        <property type="entry name" value="DUF2726"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10881">
        <property type="entry name" value="DUF2726"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF2726" evidence="2">
        <location>
            <begin position="36"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="160"/>
            <end position="181"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF10881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF08624.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="181" mass="19683" checksum="8832A452A40383B5" modified="2006-05-30" version="1">MKPFFFLILALVLVLAVAAASRGRRQRGTNFAYARRQLMTEREVGFFHKLKKSLPQSHLFAQVSMSAIVDVKRGGLAARGRFSQKYVDFVVCSSNCRVLYVIELDDKSHNSAAAQKADAVKNEILLAAGIPIKRYSSVKVDEATLARDFQEAITAVAAGAGGTKQRQPSAEKPAGVRIDGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-10-07" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LML1</accession>
    <name>Q1LML1_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Cytochrome b561</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">cybB</name>
        <name evidence="4" type="ordered locus">Rmet_1736</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08615.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1736"/>
    <dbReference type="DNASU" id="4038538"/>
    <dbReference type="EnsemblBacteria" id="ABF08615">
        <property type="protein sequence ID" value="ABF08615"/>
        <property type="gene ID" value="Rmet_1736"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1736"/>
    <dbReference type="eggNOG" id="COG3038">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_095321_3_0_4"/>
    <dbReference type="OMA" id="LHNNVAW"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022904">
        <property type="term" value="P:respiratory electron transport chain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011577">
        <property type="entry name" value="Cyt_b561_bac/Ni-Hgenase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016174">
        <property type="entry name" value="Di-haem_cyt_TM"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01292">
        <property type="entry name" value="Ni_hydr_CYTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81342">
        <property type="entry name" value="SSF81342"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="23"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="53"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="96"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="156"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="domain" description="Ni_hydr_CYTB" evidence="3">
        <location>
            <begin position="16"/>
            <end position="185"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01292"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF08615.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="185" mass="20610" checksum="4EAF46FCFD1C00F7" modified="2006-05-30" version="1">MHLTSPHTLSLTFQPKYSLAMRALHWLVFLGAVVAVAAVEFRDVFPKGSTERAAMMAIHESAGITILVLMLLRLLVRVSQPLPPPPAGDPRWMDRAASAMHFVLYVLMLAMPVLGMLAVAWKGRTIPVYGFQLALPLAINEPYSKLAKEIHETGATFVYIFVGLHAAAALWHQFVLKDRLLRRMI</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="61" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LMN3</accession>
    <name>Q1LMN3_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative membrane protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_1712</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08593.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516445.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1712"/>
    <dbReference type="EnsemblBacteria" id="ABF08593">
        <property type="protein sequence ID" value="ABF08593"/>
        <property type="gene ID" value="Rmet_1712"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1712"/>
    <dbReference type="eggNOG" id="ENOG502Z9YU">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_803426_0_0_4"/>
    <dbReference type="OMA" id="HLWMFFL"/>
    <dbReference type="OrthoDB" id="1202781at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022604">
        <property type="entry name" value="DUF2955"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11168">
        <property type="entry name" value="DUF2955"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="25"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="68"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="95"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="117"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="146"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="188"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="235"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="259"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="285"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="314"/>
            <end position="336"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF08593.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="354" mass="37123" checksum="91D209E70CF50522" modified="2006-05-30" version="1">MSTDAGASAAATAATAARSRRALRLAVGTALCLATSFGFQWAIPMVAPVLAVFLFATVGRPLPAKAGLALALLVIATTGTGLLLIPFLRHAPLSGVLMTALGLFLAFRYGLRGGNGLVSTFLVAGIALISVAGTADMGLALSVIGALARGLLLGVVMLAVCHWLIPEPGTATPAAKPPAPVASDADWMAWRATLVVMPAFLLAMADPAGYLPIVMKAVSLGRQSCTTARSAATELLGSTLLGGLLAILFWFALGLSVNLWMFFLWTILFALVVARRLFAIRPTRATPGFWLNSLVTMIILLGQSVEDSAAGKDVYHAFAVRMGLFIGVSLYGVFMLRWLDHRRQRTVSTASTTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-10-07" version="51" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LMQ1</accession>
    <name>Q1LMQ1_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_1694</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08575.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF08575">
        <property type="protein sequence ID" value="ABF08575"/>
        <property type="gene ID" value="Rmet_1694"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1694"/>
    <dbReference type="eggNOG" id="ENOG502Z8FI">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_890582_0_0_4"/>
    <dbReference type="OMA" id="DRPVRYV"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF08575.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="357" mass="38722" checksum="9A7C79B206F945A0" modified="2006-05-30" version="1">MEPWSNTRANGPTVRFRQVQALHDISDERMDRVTKRQHRPGMFSALACRGALLLVMATAVPAAHAQAQIFGPGVQRQANAVLSLMSYSVVPDLASSSLSIKSAQTDNPGIVMSQLGGGFTMSKTFPLYLEGSIAYSRYDPTFVSTDGAESRLLPTRWNTFAGTVGIGWDFPLTDDKELVIRPILNASLGNVSSDLKLATAVVQHVTDRDINFLNGGTLNAYGLGGALMLDWEHYRPEHDIDIELRYTSIHLQSFGGSAPAVQGSVTSQTANLYARWRAPTGLIALDRPLRYVLELSHSHYFGAQAGVLGFNYLTTLGAGLELDTTAHEVYVTRVRAVVRYVFGNNVSGVSLGLAASF</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LN69</accession>
    <name>Q1LN69_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">NAD-reducing hydrogenase hoxS delta subunit</fullName>
            <ecNumber evidence="3">1.12.1.2</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">hoxY</name>
        <name evidence="3" type="ordered locus">Rmet_1524</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EC" id="1.12.1.2" evidence="3"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08407.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1524"/>
    <dbReference type="EnsemblBacteria" id="ABF08407">
        <property type="protein sequence ID" value="ABF08407"/>
        <property type="gene ID" value="Rmet_1524"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1524"/>
    <dbReference type="eggNOG" id="COG1941">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_093095_0_0_4"/>
    <dbReference type="OMA" id="CNAENVH"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047985">
        <property type="term" value="F:hydrogen dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.700">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006137">
        <property type="entry name" value="NADH_UbQ_OxRdtase-like_20kDa"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037024">
        <property type="entry name" value="NiFe_Hase_small_N_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01058">
        <property type="entry name" value="Oxidored_q6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0371">Homeobox</keyword>
    <keyword evidence="1 3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Oxidored_q6" evidence="2">
        <location>
            <begin position="41"/>
            <end position="192"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01058"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF08407.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="209" mass="22760" checksum="A2118BBAC4E8C6A8" modified="2006-05-30" version="1">MSTAAKNELASHELPATPMDPALAANREGKIKVSMIGLCGCWGCTLSFLDMDERLLPLLEKITILRSSLTDIKRIPERCAIGFVEGGVANEENIETLEHFRENCDILISVGACAVWGGVPAMRNVVELKDCLAEAYVNSATAVAGAKAVIPFHPDIPRITTKVYPCHEVVKMDYFIPGCPPDGDAIFKVLDDLVNGRPFDLPSSINRYD</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LNE9</accession>
    <name>Q1LNE9_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Periplasmic chaperone</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">hlpA</name>
        <name evidence="5" type="ordered locus">Rmet_1444</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the skp family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08327.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516194.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1444"/>
    <dbReference type="EnsemblBacteria" id="ABF08327">
        <property type="protein sequence ID" value="ABF08327"/>
        <property type="gene ID" value="Rmet_1444"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1444"/>
    <dbReference type="eggNOG" id="COG2825">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_101388_1_0_4"/>
    <dbReference type="OMA" id="EKEQYDM"/>
    <dbReference type="OrthoDB" id="1854604at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.910.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005632">
        <property type="entry name" value="Chaperone_Skp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024930">
        <property type="entry name" value="Skp_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35089">
        <property type="entry name" value="PTHR35089"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03938">
        <property type="entry name" value="OmpH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002094">
        <property type="entry name" value="OMP26_Skp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00935">
        <property type="entry name" value="OmpH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111384">
        <property type="entry name" value="SSF111384"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193639" evidence="4">
        <location>
            <begin position="30"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="93"/>
            <end position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRNR" id="PIRNR002094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABF08327.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="177" mass="20066" checksum="A82430DB69DE9D44" modified="2006-05-30" version="1">MKIYTPLAKSLSAAALAAAALCAAAPAMAQEARIAAVNSERILRDSQPAKAAQVKLEQEFSKRDRELQDMAQKIKAMADKLDKDTAVLADSDRQRRQREVADLDREFQRKQREFREDLNQRRNEELAQVLERANRVIRQIAEQRKYDLIVQEAVYVNPRIDITDDVMKALNAGSTGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LNK0</accession>
    <name>Q1LNK0_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">MFS transporter, DHA2 family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Rmet_1393</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08276.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516151.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1393"/>
    <dbReference type="EnsemblBacteria" id="ABF08276">
        <property type="protein sequence ID" value="ABF08276"/>
        <property type="gene ID" value="Rmet_1393"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1393"/>
    <dbReference type="eggNOG" id="COG0477">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000960_28_0_4"/>
    <dbReference type="OMA" id="PMYLQLV"/>
    <dbReference type="OrthoDB" id="1108236at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004638">
        <property type="entry name" value="EmrB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="SSF103473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00711">
        <property type="entry name" value="efflux_EmrB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="48"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="88"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="120"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="174"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="202"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="241"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="263"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="304"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="336"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="369"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="482"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="domain" description="MFS" evidence="7">
        <location>
            <begin position="50"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ABF08276.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="508" mass="53340" checksum="9313ECB43DF04827" modified="2006-05-30" version="1">MKRESTAPAGTGSGESGGGNHGGGGGGPRRGGPTRAQLRARYGDRLRWLILLTLMLGTVSSIVSSTIVNVAIPDLSRHFLLGQERAQWVAASFMIAMTLSMLLTPWLLNRYGLRRTFIGALALLGAGGLIGGVAPTFGVMLAMRVAEGVAAGIMQPLPNIFILRVFDEREQGKALSLFGFGVVLAPAVGPSVGGFLVETFGWRSIFFVVVPLTLIALAMARRFMAVDSAMMGERKPLDWRGLMLVGVATVSLLNGLVEMRGDVHSGIGLVALGVVMLAGFVFWQLRAPYPLMNMRLYSYQQFSAGAIVAFIYGAGLFGSTYLLPVYMQMALSYTPSAAGIVLMPAGVALAVTIAVAGRLTSRVPPHRQVSFGLALLAGSFLLMATGSTATPYLLLVLYAVIGRIGLGCVLPSLTMGSMRGVDFSMIAQGSSCINFVRQLGGAIGVSLAGVGLQWRLAEHGAVLGKGGDVAAQAARIQAFDETFLAVGVVIATAILAAWRIRPRPVPAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LPA0</accession>
    <name>Q1LPA0_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">NAD binding site:FAD dependent oxidoreductase</fullName>
            <ecNumber evidence="2">1.1.99.2</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_1140</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EC" id="1.1.99.2" evidence="2"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08026.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515927.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1140"/>
    <dbReference type="EnsemblBacteria" id="ABF08026">
        <property type="protein sequence ID" value="ABF08026"/>
        <property type="gene ID" value="Rmet_1140"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1140"/>
    <dbReference type="eggNOG" id="COG0579">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024775_1_1_4"/>
    <dbReference type="OMA" id="GVHFTRM"/>
    <dbReference type="OrthoDB" id="1371190at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047545">
        <property type="term" value="F:2-hydroxyglutarate dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006076">
        <property type="entry name" value="FAD-dep_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01266">
        <property type="entry name" value="DAO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="SSF51905"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DAO" evidence="1">
        <location>
            <begin position="5"/>
            <end position="365"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF08026.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="368" mass="38866" checksum="C980599839C2A9E3" modified="2006-05-30" version="1">MEQVDCVVIGAGVVGLAVARALAPQGREVIILEAENAFGTITSARNSEVIHAGIYYPAGSLKAELCVRGKTMLYDYCASHHVAHQRCGKLIVATSTAQVATLEGIRAKAAANGVHDMQLLTRDEARAMEPQLECEAALLSPSTGIIDSHGLMTALLGDAERAGAMLAVQSPVLSGAVTPDGIRLEVGSDDGSTVLLARTVVNSAGLTAPDLARRIDGIPPEHIPPQYYAKGCYFTLAGRAPFSRLIYPVPEAAGLGVHLTLDMGGQARFGPNVRWIDEIEYSVPPHDADSFYDEVRRYWPGLADGALQPGYAGIRPKISGPTEVAADFRIDGPRTHGVPGLVNLFGIESPGLTSSLAIAERVAELLEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LPG1</accession>
    <name>Q1LPG1_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">tRNA (cytidine/uridine-2'-O-)-methyltransferase TrmJ</fullName>
            <ecNumber evidence="5">2.1.1.200</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">tRNA (cytidine(32)/uridine(32)-2'-O)-methyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="5">tRNA Cm32/Um32 methyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5" type="primary">trmJ</name>
        <name evidence="7" type="ordered locus">Rmet_1079</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Catalyzes the formation of 2'O-methylated cytidine (Cm32) or 2'O-methylated uridine (Um32) at position 32 in tRNA.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>S-adenosyl-L-methionine + uridine(32) in tRNA = 2'-O-methyluridine(32) in tRNA + H(+) + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:42936"/>
            <dbReference type="Rhea" id="RHEA-COMP:10107"/>
            <dbReference type="Rhea" id="RHEA-COMP:10290"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="ChEBI" id="CHEBI:74478"/>
            <dbReference type="EC" id="2.1.1.200"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>cytidine(32) in tRNA + S-adenosyl-L-methionine = 2'-O-methylcytidine(32) in tRNA + H(+) + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:42932"/>
            <dbReference type="Rhea" id="RHEA-COMP:10288"/>
            <dbReference type="Rhea" id="RHEA-COMP:10289"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:74495"/>
            <dbReference type="ChEBI" id="CHEBI:82748"/>
            <dbReference type="EC" id="2.1.1.200"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="5">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class IV-like SAM-binding methyltransferase superfamily. RNA methyltransferase TrmH family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.200" evidence="5"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07965.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515867.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1079"/>
    <dbReference type="EnsemblBacteria" id="ABF07965">
        <property type="protein sequence ID" value="ABF07965"/>
        <property type="gene ID" value="Rmet_1079"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1079"/>
    <dbReference type="eggNOG" id="COG0565">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_056931_0_0_4"/>
    <dbReference type="OMA" id="PIMNLSH"/>
    <dbReference type="OrthoDB" id="1637132at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008173">
        <property type="term" value="F:RNA methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008033">
        <property type="term" value="P:tRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1280.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029028">
        <property type="entry name" value="Alpha/beta_knot_MTases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004384">
        <property type="entry name" value="RNA_MeTrfase_TrmJ/LasT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001537">
        <property type="entry name" value="SpoU_MeTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029026">
        <property type="entry name" value="tRNA_m1G_MTases_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42786">
        <property type="entry name" value="PTHR42786"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00588">
        <property type="entry name" value="SpoU_methylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004808">
        <property type="entry name" value="LasT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75217">
        <property type="entry name" value="SSF75217"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00050">
        <property type="entry name" value="rRNA_methyl_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 5 7" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="4 7" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="SpoU_methylase" evidence="6">
        <location>
            <begin position="28"/>
            <end position="187"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU362024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABF07965.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="289" mass="30442" checksum="E5EA2C0490158255" modified="2006-05-30" version="1">MTEVLNSQMDPIPRGAAASDLSDIFAHVRVVLVETSHPGNVGSVARAIKTMGFGHVPGSLVLVSPREPGVVSHPDAVAMASGADDVLASATIVDSIEAALAGTAFTVAMTARQREFGPPRLLPRAAVAKARSVLEADPATTVAFVFGNERFGLPNDAVLRCSAVTHIPANPVYASLNLAQAVQLIAYEMRLALLEAQETAGGLPDVAGDIGYAGDPATTEQIEAMFGHLQAGLAAIGFLDPANPRKLMPRLRRLFARTGLEREEVNILRGIAKRMLLTGDAKHDDNKED</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="68" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LPG8</accession>
    <name>Q1LPG8_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">JmjC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_1072</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07958.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515860.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1072"/>
    <dbReference type="EnsemblBacteria" id="ABF07958">
        <property type="protein sequence ID" value="ABF07958"/>
        <property type="gene ID" value="Rmet_1072"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1072"/>
    <dbReference type="eggNOG" id="COG2850">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039125_1_0_4"/>
    <dbReference type="OMA" id="VDQFVPE"/>
    <dbReference type="OrthoDB" id="1885303at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003347">
        <property type="entry name" value="JmjC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039994">
        <property type="entry name" value="JmjC_protein"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13096">
        <property type="entry name" value="PTHR13096"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08007">
        <property type="entry name" value="Cupin_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51184">
        <property type="entry name" value="JMJC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="JmjC" evidence="2">
        <location>
            <begin position="88"/>
            <end position="214"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF07958.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="375" mass="42227" checksum="135807E08D493392" modified="2006-05-30" version="1">MTPSAFMRDIWHRKPLLIRQAVPEIVPPVSREALFELADRDDVESRLVSFFRNRWKLEHGPFSEDNLPSRKTGKWSLLVQGVNLHDRAAAELMSQFRFVPDARLDDVMISYATDGGGVGPHFDSYDVFLLQVSGRRRWRISSQTKLDLIPDLPLKILSDFSAEQEWVLEPGDMLYLPPQYAHDGVAEGECMTASIGFRAPAYRELAGHFLAWLSETVADNEGLDGRYADAGESATSTPAQLPANLARAVAERLQSLKWNAGMVSEFLGTHLSEPKPSVQFDEIEEIPLRQFTKLARTHGVVLAPASVALYDRTHFFLNGEAYEPPASLAKWLRRLADRRQLTASEVEACVALPDLMDTFHDWAMEGWLQIAPKRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LPH0</accession>
    <name>Q1LPH0_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Metal-dependent hydrolase of the beta-lactamase superfamily I</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_1070</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07956.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515857.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1070"/>
    <dbReference type="EnsemblBacteria" id="ABF07956">
        <property type="protein sequence ID" value="ABF07956"/>
        <property type="gene ID" value="Rmet_1070"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1070"/>
    <dbReference type="eggNOG" id="COG1235">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_073253_1_0_4"/>
    <dbReference type="OMA" id="VLECNHD"/>
    <dbReference type="OrthoDB" id="733552at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001279">
        <property type="entry name" value="Metallo-B-lactamas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12706">
        <property type="entry name" value="Lactamase_B_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00849">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="SSF56281"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Lactamase_B" evidence="1">
        <location>
            <begin position="12"/>
            <end position="195"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF07956.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="262" mass="28077" checksum="F418F5A93D71F00B" modified="2006-05-30" version="1">MMRFAFLGSGSEGNSLLIEASDGASVTRVLLDCGFGIKETARRLERLGVAPEQLDAVLVTHEHGDHVGSAYAFAARYKLPVHTSHGTWLATSHMRGADVADVRVCCADHSFSVGGLQIHPYTVPHDAREPLQFVLTDGDARLGVLTDAGMETPYVTARLAGVHSLVLECNHDREMLRNSVYPYSLKRRIGGDFGHLANDIAASILSQVMHGQMSRVVAAHLSKQNNTCELAAGALASVLGTSPGDILIADQEEGLGWQAVRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LPK5</accession>
    <name>Q1LPK5_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Short-chain alcohol dehydrogenases SDR</fullName>
            <ecNumber evidence="1">1.-.-.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_1035</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EC" id="1.-.-.-" evidence="1"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07921.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008649271.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1035"/>
    <dbReference type="EnsemblBacteria" id="ABF07921">
        <property type="protein sequence ID" value="ABF07921"/>
        <property type="gene ID" value="Rmet_1035"/>
    </dbReference>
    <dbReference type="GeneID" id="60821896"/>
    <dbReference type="KEGG" id="rme:Rmet_1035"/>
    <dbReference type="eggNOG" id="COG1028">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010194_2_11_4"/>
    <dbReference type="OMA" id="PYKGWSA"/>
    <dbReference type="OrthoDB" id="1261526at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF07921.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="256" mass="27391" checksum="C2E0EA3AB5D9B249" modified="2006-05-30" version="1">MTTGTPRSTHLYIVTGASRGLGAALVRALLVPGNRVIGVARSRNPELEAEATASGVRVAWHLQDLSQPGPSAGWLASVLDAVEEAPASITLILNAGVVEPIGPITQLHDSTLVPHLQTNLVTPMTMTGAFIEHTVRFDCPRKVLAISSGAARNPVPGWSAYCAGKAGLDMFIRSVNTEYASVPEPRTLRAVALAPGVVDTGMQETIRGADFAQVQRFRDLKDNEQLASPDDTARRIVAYLARPDFGTTELDDIRKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="70" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LPZ9</accession>
    <name>Q1LPZ9_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Xanthine dehydrogenase, maturation factor</fullName>
            <ecNumber evidence="3">1.17.1.4</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">xdhC</name>
        <name evidence="3" type="ordered locus">Rmet_0891</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EC" id="1.17.1.4" evidence="3"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07777.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515717.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0891"/>
    <dbReference type="EnsemblBacteria" id="ABF07777">
        <property type="protein sequence ID" value="ABF07777"/>
        <property type="gene ID" value="Rmet_0891"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0891"/>
    <dbReference type="eggNOG" id="COG1975">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041115_4_0_4"/>
    <dbReference type="OMA" id="YCIVMTH"/>
    <dbReference type="OrthoDB" id="1879870at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004854">
        <property type="term" value="F:xanthine dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014308">
        <property type="entry name" value="Xanthine_DH_XdhC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003777">
        <property type="entry name" value="XdhC_CoxI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027051">
        <property type="entry name" value="XdhC_Rossmann_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13478">
        <property type="entry name" value="XdhC_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02625">
        <property type="entry name" value="XdhC_CoxI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02964">
        <property type="entry name" value="xanthine_xdhC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="XdhC_CoxI" evidence="1">
        <location>
            <begin position="17"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="domain" description="XdhC_C" evidence="2">
        <location>
            <begin position="169"/>
            <end position="310"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02625"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF07777.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="331" mass="35846" checksum="598C47CADA0B7DA5" modified="2006-05-30" version="1">MQDAPLKPFRFADATRWVRAGVPVAMVTIVEVKGSAPREAGIRMLVTADDLVGTIGGGHLEWRGMDIAREMIAKGENGEQRRIERIPLGPALGQCCGGVVRLAFEVLTHDDLAWLDAVESAFATGKALARHVPVTGAVRTEASRDLLPSVQLADDSWIDTLVPNDMHIVLFGAGHVGHALVKVLATLPCRVHWVDERDTLFPAGLPENVEAEATDTAEAVVDQAPAGSYFLVMTHSHALDQTLCERIFRRTDFAYFGLIGSKTKRAKFEHRMAEHGIDAARFPEMTCPMGVSGITDKAPAMIAVAIVAQLLQVRDQRAAALAASRREAVHP</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-10-07" version="61" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LQ26</accession>
    <name>Q1LQ26_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_0864</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07750.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0864"/>
    <dbReference type="EnsemblBacteria" id="ABF07750">
        <property type="protein sequence ID" value="ABF07750"/>
        <property type="gene ID" value="Rmet_0864"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0864"/>
    <dbReference type="eggNOG" id="COG4320">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032121_0_0_4"/>
    <dbReference type="OMA" id="QFRDMKD"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018721">
        <property type="entry name" value="DUF2252"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39441">
        <property type="entry name" value="PTHR39441"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10009">
        <property type="entry name" value="DUF2252"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF07750.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="498" mass="55921" checksum="2272EDFEC1DB9020" modified="2006-05-30" version="1">MTSCRRSLSVVAYAAQIRNVVTLRHGGCVENVAEVEERRARGKALRQKVQRRDHAVIGNVDRDPISLLEESSAGRVSRLIPLRYGRMIASPFTFYRGSAIIQAHDLAGTPNSSLTMQICGDCHLMNFGGFATPERALLFDVNDFDETSPGPWEWDLKRLAASFVVAARHLRHGATAADNMVYQVANSYRDRMAEYAEMAILDTWYDRITFERLLESAVNPEVQKRIRRAMERAAARTHESLLPKLTEHDGDRWRILDAPPAVFHVHGAQTLFETDDDWLSIDDPSKLIDQSFSDYKSTLAADRRRLLDHFKLQDMAFKVVGVGSVGTRCLILLLIDGHGKPLFLQVKEATKSVVARYFKSAAVSHEGRRVVDGQRLMQAASDLFLGWSKGPFGRHFYIRQLRDMKFSVDIELMDADLLATYGQVCGWVLARAHAKASGHAAEISGYLGASDRMAESLIAYSNAYADQVERDYDLFVKACRSGRLEARTDVDMAADFRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LR20</accession>
    <name>Q1LR20_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Methylated-DNA (Protein)-cysteine S-methyltransferase</fullName>
            <ecNumber evidence="7">2.1.1.63</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">ogt1</name>
        <name evidence="7" type="ordered locus">Rmet_0520</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a 4-O-methyl-thymidine in DNA + L-cysteinyl-[protein] = a thymidine in DNA + S-methyl-L-cysteinyl-[protein]</text>
            <dbReference type="Rhea" id="RHEA:53428"/>
            <dbReference type="Rhea" id="RHEA-COMP:10131"/>
            <dbReference type="Rhea" id="RHEA-COMP:10132"/>
            <dbReference type="Rhea" id="RHEA-COMP:13555"/>
            <dbReference type="Rhea" id="RHEA-COMP:13556"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:82612"/>
            <dbReference type="ChEBI" id="CHEBI:137386"/>
            <dbReference type="ChEBI" id="CHEBI:137387"/>
            <dbReference type="EC" id="2.1.1.63"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>a 6-O-methyl-2'-deoxyguanosine in DNA + L-cysteinyl-[protein] = a 2'-deoxyguanosine in DNA + S-methyl-L-cysteinyl-[protein]</text>
            <dbReference type="Rhea" id="RHEA:24000"/>
            <dbReference type="Rhea" id="RHEA-COMP:10131"/>
            <dbReference type="Rhea" id="RHEA-COMP:10132"/>
            <dbReference type="Rhea" id="RHEA-COMP:11367"/>
            <dbReference type="Rhea" id="RHEA-COMP:11368"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:82612"/>
            <dbReference type="ChEBI" id="CHEBI:85445"/>
            <dbReference type="ChEBI" id="CHEBI:85448"/>
            <dbReference type="EC" id="2.1.1.63"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.1.1.63" evidence="7"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07406.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008645026.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0520"/>
    <dbReference type="EnsemblBacteria" id="ABF07406">
        <property type="protein sequence ID" value="ABF07406"/>
        <property type="gene ID" value="Rmet_0520"/>
    </dbReference>
    <dbReference type="GeneID" id="60824117"/>
    <dbReference type="KEGG" id="rme:Rmet_0520"/>
    <dbReference type="eggNOG" id="COG0350">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_52_2_4"/>
    <dbReference type="OMA" id="QANRANE"/>
    <dbReference type="OrthoDB" id="1327994at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003908">
        <property type="term" value="F:methylated-DNA-[protein]-cysteine S-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06445">
        <property type="entry name" value="ATase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001497">
        <property type="entry name" value="MethylDNA_cys_MeTrfase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014048">
        <property type="entry name" value="MethylDNA_cys_MeTrfase_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036217">
        <property type="entry name" value="MethylDNA_cys_MeTrfase_DNAb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036631">
        <property type="entry name" value="MGMT_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01035">
        <property type="entry name" value="DNA_binding_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46767">
        <property type="entry name" value="SSF46767"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53155">
        <property type="entry name" value="SSF53155"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00589">
        <property type="entry name" value="ogt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00374">
        <property type="entry name" value="MGMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0227">DNA damage</keyword>
    <keyword evidence="5" id="KW-0234">DNA repair</keyword>
    <keyword evidence="3 7" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="DNA_binding_1" evidence="6">
        <location>
            <begin position="73"/>
            <end position="155"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001596"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABF07406.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="159" mass="17327" checksum="435FEA6EB0C300B2" modified="2006-05-30" version="1">MQHHFDAVLPAPFGKVGVRVDGTQVHEIVYLPDSFAEVPPTSALTKRVAEQLDAYYANADTVFDLPLARRGTDFQRKVWAAISAVPRGGLTTYGTIAKSLQSMPRAVGQACGQNWFPIVVPCHRVVAANGLGGFAHHGEEGFHLGVKRWLLRHEGAMLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="55" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LR72</accession>
    <name>Q1LR72_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_0468</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07354.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0468"/>
    <dbReference type="EnsemblBacteria" id="ABF07354">
        <property type="protein sequence ID" value="ABF07354"/>
        <property type="gene ID" value="Rmet_0468"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0468"/>
    <dbReference type="eggNOG" id="ENOG5030717">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_083007_0_0_4"/>
    <dbReference type="OMA" id="FQLDRCH"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021312">
        <property type="entry name" value="DUF2889"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11136">
        <property type="entry name" value="DUF2889"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="216"/>
            <end position="253"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF07354.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="253" mass="27622" checksum="64FAFE1AD09511A5" modified="2006-05-30" version="1">MVVLPMDQAVCRDELPPIRHFQSAASAPGVMPLSPPANRVLRHRRAITAEAYLRDDGLWDIEARLTDTKPRDIELAGGRIRPEGQPLHDLWLRVTIDLRMNVVDAEACSDWVPYPTHCDLIGPAYRKLIGLNLSKGFRKAVRERLGSVAGCTHLTELAGVLPTAAIQAFAGDVFNIRDGVQDIDPEPPMQLHGCHALHFEGEVVRQFYPRWYGHQPKPKAGAAASSPQTASRADAPASGGEDTLAHRPNGTSG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-01-15" modified="2022-08-03" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LRB4</accession>
    <name>RUVC_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Crossover junction endodeoxyribonuclease RuvC</fullName>
            <ecNumber evidence="1">3.1.21.10</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Holliday junction nuclease RuvC</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Holliday junction resolvase RuvC</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">ruvC</name>
        <name type="ordered locus">Rmet_0426</name>
    </gene>
    <organism>
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Nuclease that resolves Holliday junction intermediates in genetic recombination. Cleaves the cruciform structure in supercoiled DNA by nicking to strands with the same polarity at sites symmetrically opposed at the junction in the homologous arms and leaves a 5'-terminal phosphate and a 3'-terminal hydroxyl group.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Endonucleolytic cleavage at a junction such as a reciprocal single-stranded crossover between two homologous DNA duplexes (Holliday junction).</text>
            <dbReference type="EC" id="3.1.21.10"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="1">Binds 1 Mg(2+) ion per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the RuvC family.</text>
    </comment>
    <dbReference type="EC" id="3.1.21.10" evidence="1"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07312.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008646027.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q1LRB4"/>
    <dbReference type="SMR" id="Q1LRB4"/>
    <dbReference type="STRING" id="266264.Rmet_0426"/>
    <dbReference type="EnsemblBacteria" id="ABF07312">
        <property type="protein sequence ID" value="ABF07312"/>
        <property type="gene ID" value="Rmet_0426"/>
    </dbReference>
    <dbReference type="GeneID" id="60824028"/>
    <dbReference type="KEGG" id="rme:Rmet_0426"/>
    <dbReference type="eggNOG" id="COG0817">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_091257_3_1_4"/>
    <dbReference type="OMA" id="AICHIWR"/>
    <dbReference type="OrthoDB" id="1815080at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008821">
        <property type="term" value="F:crossover junction endodeoxyribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd16962">
        <property type="entry name" value="RuvC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00034">
        <property type="entry name" value="RuvC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020563">
        <property type="entry name" value="X-over_junc_endoDNase_Mg_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002176">
        <property type="entry name" value="X-over_junc_endoDNase_RuvC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30194">
        <property type="entry name" value="PTHR30194"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02075">
        <property type="entry name" value="RuvC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00696">
        <property type="entry name" value="RSOLVASERUVC"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="SSF53098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00228">
        <property type="entry name" value="ruvC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01321">
        <property type="entry name" value="RUVC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0227">DNA damage</keyword>
    <keyword id="KW-0233">DNA recombination</keyword>
    <keyword id="KW-0234">DNA repair</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0540">Nuclease</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_1000002808" description="Crossover junction endodeoxyribonuclease RuvC">
        <location>
            <begin position="1"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="7"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="67"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="139"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="142"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00034"/>
        </source>
    </evidence>
    <sequence length="181" mass="19020" checksum="D707A6454B88A1CE" modified="2006-05-30" version="1">MRILGIDPGLRTTGFGVLEKHGNKLAYVASGTIRSNGETSLPERLKTLYDGISEVARTYAPDCAAIEKVFVNVNPQSTLLLGQARGAAICGLVGQGLPVFEYTALQLKVAVVGYGRANKEQVQEMVMRLLSLSGRPSSDAADALGVAICHANGGDTLGTLAGLAPELARKGMRVRRGRLVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LRG8</accession>
    <name>Q1LRG8_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Import inner membrane translocase, subunit Tim44</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Rmet_0372</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07258.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515249.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0372"/>
    <dbReference type="EnsemblBacteria" id="ABF07258">
        <property type="protein sequence ID" value="ABF07258"/>
        <property type="gene ID" value="Rmet_0372"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0372"/>
    <dbReference type="eggNOG" id="COG4395">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_052470_0_0_4"/>
    <dbReference type="OMA" id="HWDANEM"/>
    <dbReference type="OrthoDB" id="1536134at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032710">
        <property type="entry name" value="NTF2-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007379">
        <property type="entry name" value="Tim44-like_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04280">
        <property type="entry name" value="Tim44"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00978">
        <property type="entry name" value="Tim44"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54427">
        <property type="entry name" value="SSF54427"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193461" evidence="3">
        <location>
            <begin position="28"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="91"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="115"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="domain" description="Tim44" evidence="4">
        <location>
            <begin position="196"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="33"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="33"/>
            <end position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00978"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABF07258.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="329" mass="33566" checksum="504B320F03281882" modified="2006-05-30" version="1">MSSFRGKFLAMSLIATLALGAALDANAKRLGGSRSIGKQSSGVTQQRQQAPAQQNTPPAQQPAQAAPATAGAAGAAGGAAAAAAPKRNWGGMLGGLAAGLGIGYLLSHFGLGGAAASILSNVILFGVIALIAMWLIRKFRGGSARTQQPSYAGMGGNGSNYGGPSHAEPTLRTAEPVGNGGAASNPVMGGAAAAATAAAVQQPWGVPADFDTESFLRNAKVHYVRLQAAWDAGNQDDIREFTTPEMFAEIKMDLSERGSEVNKTDVVTLDAQLLGIESTPAQHIASVRFSGMIREKAGEPAQPFGEVWNLAKATSGNGGWLLAGIQQES</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LRI5</accession>
    <name>Q1LRI5_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Trehalose 6-phosphate phosphatase</fullName>
            <ecNumber evidence="3">3.1.3.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">otsB</name>
        <name evidence="4" type="ordered locus">Rmet_0355</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Removes the phosphate from trehalose 6-phosphate to produce free trehalose.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>alpha,alpha-trehalose 6-phosphate + H2O = alpha,alpha-trehalose + phosphate</text>
            <dbReference type="Rhea" id="RHEA:23420"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16551"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58429"/>
            <dbReference type="EC" id="3.1.3.12"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="1 3">Glycan biosynthesis; trehalose biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 3">Belongs to the trehalose phosphatase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.12" evidence="3"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07241.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0355"/>
    <dbReference type="EnsemblBacteria" id="ABF07241">
        <property type="protein sequence ID" value="ABF07241"/>
        <property type="gene ID" value="Rmet_0355"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0355"/>
    <dbReference type="eggNOG" id="COG1877">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037265_2_0_4"/>
    <dbReference type="OMA" id="YGAERWD"/>
    <dbReference type="UniPathway" id="UPA00299"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004805">
        <property type="term" value="F:trehalose-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005992">
        <property type="term" value="P:trehalose biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006379">
        <property type="entry name" value="HAD-SF_hydro_IIB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044651">
        <property type="entry name" value="OTSB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003337">
        <property type="entry name" value="Trehalose_PPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43768">
        <property type="entry name" value="PTHR43768"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02358">
        <property type="entry name" value="Trehalose_PPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="SSF56784"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01484">
        <property type="entry name" value="HAD-SF-IIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00685">
        <property type="entry name" value="T6PP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU361117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF07241.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="272" mass="28706" checksum="5D75B26667370130" modified="2006-05-30" version="1">MLRSCVMSSLPLIEPDTALFLDFDGTLADLAPRPELVQVEPELVGTLRALHTCLDGALAIISGRPVAELDGFLQPLRLPAAGVHGAELRHDGEALFMPPAPGITALMPRLEAFVARHPGLRIERKSVAVAIHYRQAPELEAQVRAMVADTLHDVDGLEGLPGKMVVEIKPAGVDKGGAIAAFMRMAPFEGRKPLFAGDDVTDEAGFVVARELGGVGVLVGERATAATVSVTGPAALRCWLHRSAHALLALRAQRGGKPQSVSSTSSETEDSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LRJ4</accession>
    <name>Q1LRJ4_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Aminotransferase</fullName>
            <ecNumber evidence="1">2.6.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_0346</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.6.1.-" evidence="1"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07232.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515225.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0346"/>
    <dbReference type="EnsemblBacteria" id="ABF07232">
        <property type="protein sequence ID" value="ABF07232"/>
        <property type="gene ID" value="Rmet_0346"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0346"/>
    <dbReference type="eggNOG" id="COG0436">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017584_4_3_4"/>
    <dbReference type="OMA" id="WIVADEV"/>
    <dbReference type="OrthoDB" id="554560at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008483">
        <property type="term" value="F:transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901605">
        <property type="term" value="P:alpha-amino acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009058">
        <property type="term" value="P:biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004838">
        <property type="entry name" value="NHTrfase_class1_PyrdxlP-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="SSF53383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00105">
        <property type="entry name" value="AA_TRANSFER_CLASS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 3" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aminotran_1_2" evidence="2">
        <location>
            <begin position="53"/>
            <end position="393"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU000481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF07232.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="399" mass="43176" checksum="E0ADAAA65EB81D8F" modified="2006-05-30" version="1">MSTSPSRQSSLTEAPPRARDVVHALRASRIREVANAGIGLPDVLPFWFGESDQVTPSFIRDAASRALANGATFYNHNLGIAPLRAALSEYVTRLHGATPVDHIAVTSAGVNALMLAAQLVTGPGDRVVAVTPLWPNLVEIPRILGAAVETVTLDYGADGWTLDVDKLLAALTPGTRAVMINSPNNPTGWVMPRGAQQAVLDHCRRHGIWIIADEVYERLYYGDEGAVAPSFLDIAGRDERVIAVNSFSKAWLMTGWRLGWMVVPSSMMDDLGKLIEYNTSCAPSFVQEAGVVAVREGEAFTQDLVKRLRSARDHLVSALSALPGIDAHAPDGAMYVFFRLKGATDSLDLCKRLVRDAHLGLAPGSAFGDEGEGFVRWCYACDEARLDEGVKRLRGYLGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="67" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LRJ5</accession>
    <name>Q1LRJ5_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_0345</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07231.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515224.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0345"/>
    <dbReference type="EnsemblBacteria" id="ABF07231">
        <property type="protein sequence ID" value="ABF07231"/>
        <property type="gene ID" value="Rmet_0345"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0345"/>
    <dbReference type="eggNOG" id="COG3246">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_065536_3_0_4"/>
    <dbReference type="OMA" id="WAICAFG"/>
    <dbReference type="OrthoDB" id="1892901at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019475">
        <property type="term" value="P:L-lysine catabolic process to acetate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008567">
        <property type="entry name" value="KCE_family"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37418">
        <property type="entry name" value="PTHR37418"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05853">
        <property type="entry name" value="BKACE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF07231.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="289" mass="31016" checksum="811F3FADB1FEEC69" modified="2006-05-30" version="1">MPNQNPVATADTLRRPFALAVAPNGARKTHADHACLPITPDELAQCARECVDAGAAMIHLHVRKADATHSLEVVDYEPAIAAVRKAVGNALVLQLTTEAVGIYQPAQQIATVRALRPEAASVAIRELVPNVAALPAAAEFFQWMHHEHVVAQYILYDADDVRRYAALRESGAIPAGRHWVLFVLGRYSAGQKSSPADLLPFLAAWRETGLDQSGVEWAVCAFGKQEADCAATAVMLGGHARIGFENNMTLPDGAMAPDNAALLQAVRTPLLALGYVSMTADVLRERFAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LRK1</accession>
    <name>Q1LRK1_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Monooxygenase, FAD-binding:FAD dependent oxidoreductase</fullName>
            <ecNumber evidence="2">1.14.13.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_0339</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EC" id="1.14.13.-" evidence="2"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07225.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515219.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0339"/>
    <dbReference type="EnsemblBacteria" id="ABF07225">
        <property type="protein sequence ID" value="ABF07225"/>
        <property type="gene ID" value="Rmet_0339"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0339"/>
    <dbReference type="eggNOG" id="COG0654">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009665_20_2_4"/>
    <dbReference type="OMA" id="FHSDERQ"/>
    <dbReference type="OrthoDB" id="867226at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002938">
        <property type="entry name" value="FAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01494">
        <property type="entry name" value="FAD_binding_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="SSF51905"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD_binding_3" evidence="1">
        <location>
            <begin position="34"/>
            <end position="369"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF07225.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="584" mass="63697" checksum="06A7B333A6FEFC7E" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LS88</accession>
    <name>Q1LS88_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Transcriptional regulator, MerR-family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_0102</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF06988.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515023.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0102"/>
    <dbReference type="EnsemblBacteria" id="ABF06988">
        <property type="protein sequence ID" value="ABF06988"/>
        <property type="gene ID" value="Rmet_0102"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0102"/>
    <dbReference type="eggNOG" id="COG0789">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_060077_3_1_4"/>
    <dbReference type="OMA" id="HTMLELV"/>
    <dbReference type="OrthoDB" id="1661714at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009061">
        <property type="entry name" value="DNA-bd_dom_put_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000551">
        <property type="entry name" value="MerR-type_HTH_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13411">
        <property type="entry name" value="MerR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00422">
        <property type="entry name" value="HTH_MERR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46955">
        <property type="entry name" value="SSF46955"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50937">
        <property type="entry name" value="HTH_MERR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH merR-type" evidence="2">
        <location>
            <begin position="9"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="101"/>
            <end position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF06988.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="144" mass="16461" checksum="30AB62348095B9D1" modified="2006-05-30" version="1">MSAQPSTVTYTITDLAREFDITPRAIRFYEDQGLLAPDREGPSGRRRVYNSRERTRLKLTLRGKRLGLTLNEIREILDLYESPRDTAPQLERFLHLLAGHRGTLERQLEDLQAQLAEIDQHERQCQALLAAQHAKNAGNKTPTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-04-26" modified="2022-01-19" version="102" xmlns="http://uniprot.org/uniprot">
    <accession>Q58AD6</accession>
    <name>Q58AD6_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">CopA multi-Cu(II) oxidase involved in Cu(II)/Cu(I) resistance</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">copA1</name>
        <name evidence="7" type="ordered locus">Rmet_6112</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="7 8">
        <name>pMOL30</name>
    </geneLocation>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="8">Plasmid pMOL30</plasmid>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Periplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP000354">
        <property type="protein sequence ID" value="ABF12971.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011229403.1">
        <property type="nucleotide sequence ID" value="NC_007971.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_145682.1">
        <property type="nucleotide sequence ID" value="NC_006466.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF12971">
        <property type="protein sequence ID" value="ABF12971"/>
        <property type="gene ID" value="Rmet_6112"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6112"/>
    <dbReference type="HOGENOM" id="CLU_009100_5_2_4"/>
    <dbReference type="OMA" id="KGMDHDM"/>
    <dbReference type="OrthoDB" id="971126at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid pMOL30"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042597">
        <property type="term" value="C:periplasmic space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd13848">
        <property type="entry name" value="CuRO_1_CopA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13874">
        <property type="entry name" value="CuRO_2_CopA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13896">
        <property type="entry name" value="CuRO_3_CopA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.420">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001117">
        <property type="entry name" value="Cu-oxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011706">
        <property type="entry name" value="Cu-oxidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045087">
        <property type="entry name" value="Cu-oxidase_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011707">
        <property type="entry name" value="Cu-oxidase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006376">
        <property type="entry name" value="Cu-R_CopA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033138">
        <property type="entry name" value="Cu_oxidase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002355">
        <property type="entry name" value="Cu_oxidase_Cu_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008972">
        <property type="entry name" value="Cupredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034284">
        <property type="entry name" value="CuRO_1_CopA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034282">
        <property type="entry name" value="CuRO_2_CopA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034279">
        <property type="entry name" value="CuRO_3_CopA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006311">
        <property type="entry name" value="TAT_signal"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11709">
        <property type="entry name" value="PTHR11709"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00394">
        <property type="entry name" value="Cu-oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07731">
        <property type="entry name" value="Cu-oxidase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07732">
        <property type="entry name" value="Cu-oxidase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49503">
        <property type="entry name" value="SSF49503"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01480">
        <property type="entry name" value="copper_res_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00079">
        <property type="entry name" value="MULTICOPPER_OXIDASE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00080">
        <property type="entry name" value="MULTICOPPER_OXIDASE2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51318">
        <property type="entry name" value="TAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-0614">Plasmid</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Plastocyanin-like" evidence="6">
        <location>
            <begin position="69"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="domain" description="Plastocyanin-like" evidence="4">
        <location>
            <begin position="189"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="domain" description="Plastocyanin-like" evidence="5">
        <location>
            <begin position="498"/>
            <end position="613"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF07731"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF07732"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABF12971.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="614" mass="67744" checksum="1F3B825810A3BA3B" modified="2005-04-26" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2005-04-26" modified="2022-05-25" version="108" xmlns="http://uniprot.org/uniprot">
    <accession>Q58AG1</accession>
    <name>Q58AG1_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="9">NreB (Nickel efflux) family MFS(Major facilitator superfamily)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">nreB</name>
        <name evidence="9" type="ordered locus">Rmet_6144</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="9 10">
        <name>pMOL30</name>
    </geneLocation>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="10">Plasmid pMOL30</plasmid>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP000354">
        <property type="protein sequence ID" value="ABF13003.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008642699.1">
        <property type="nucleotide sequence ID" value="NC_007971.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_145657.1">
        <property type="nucleotide sequence ID" value="NC_006466.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF13003">
        <property type="protein sequence ID" value="ABF13003"/>
        <property type="gene ID" value="Rmet_6144"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6144"/>
    <dbReference type="HOGENOM" id="CLU_034180_7_1_4"/>
    <dbReference type="OMA" id="SLSHACW"/>
    <dbReference type="OrthoDB" id="498436at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid pMOL30"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004751">
        <property type="entry name" value="Drug_antiport"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="SSF103473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00900">
        <property type="entry name" value="2A0121"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0614">Plasmid</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="46"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="168"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="219"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="252"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="282"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="313"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="346"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="376"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="domain" description="MFS" evidence="8">
        <location>
            <begin position="1"/>
            <end position="400"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ABF13003.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="408" mass="43490" checksum="2256FBE20231A62B" modified="2005-04-26" version="1">MLDVLRNRTYRHLFGAQVIALIGTGLATVALGLLAYDLAGANAGSVLGTALAIKMVAYVGIAPVVGAFANRLPRRAFLVAMDLVRALVAISLPFVTQIWQIYILIFLLQSASAAFTPTFQATIPDVLPEEKAYTNALSLSRLAYDMESLVSPMLAAALLSVIGFHWLFGGTVVGFLVSAAFVVSVRLPQSKAIARKESLYAKTFRGMSIYLRTHRLRGLLALNLAAAAASSMVIVNTVVYVQSKLQRPASDVPFALAAFGCGSMLVALLLPRLLANRPDRPAMLGGAILMSASLAFGVALTGFGQVIEWPMLLLTWFATGIGYSMTLTPSGRLLKRSAASADRPAVFAAQFSLSHACWLIAYPLAGQVGARYGMRAAFVVLACVSVVGFLAAWRVWPAANHEDTVPVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-04-26" modified="2021-09-29" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>Q58AK6</accession>
    <name>Q58AK6_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Transposase ISRme19 (DNA binding protein)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_6372</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="1 2">
        <name>pMOL30</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid pMOL30</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000354">
        <property type="protein sequence ID" value="ADC45355.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011229357.1">
        <property type="nucleotide sequence ID" value="NC_006466.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_145612.1">
        <property type="nucleotide sequence ID" value="NC_006466.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADC45355">
        <property type="protein sequence ID" value="ADC45355"/>
        <property type="gene ID" value="Rmet_6372"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6372"/>
    <dbReference type="HOGENOM" id="CLU_113764_6_1_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid pMOL30"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004803">
        <property type="term" value="F:transposase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006313">
        <property type="term" value="P:transposition, DNA-mediated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002514">
        <property type="entry name" value="Transposase_8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010921">
        <property type="entry name" value="Trp_repressor/repl_initiator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01527">
        <property type="entry name" value="HTH_Tnp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48295">
        <property type="entry name" value="SSF48295"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADC45355.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="125" mass="13816" checksum="F77206EE5B910B9E" modified="2005-04-26" version="1">MNTIEESAPARRRRRHSAEFKAKAVQDCLHRGVSIAAVALHHRVNANLLRRWVAEHQALDSAGEARALMTVPQAEFIPLQIGDPTPTPAIPDIQIEVRRGAATISIRWPGSAAAECGEWLQGWLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-04-26" modified="2021-09-29" version="70" xmlns="http://uniprot.org/uniprot">
    <accession>Q58AT1</accession>
    <name>Q58AT1_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_6059</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2 3">
        <name>pMOL30</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid pMOL30</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000354">
        <property type="protein sequence ID" value="ABF12918.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011229288.1">
        <property type="nucleotide sequence ID" value="NC_007971.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_145534.1">
        <property type="nucleotide sequence ID" value="NC_006466.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF12918">
        <property type="protein sequence ID" value="ABF12918"/>
        <property type="gene ID" value="Rmet_6059"/>
    </dbReference>
    <dbReference type="GeneID" id="60825057"/>
    <dbReference type="KEGG" id="rme:Rmet_6059"/>
    <dbReference type="HOGENOM" id="CLU_1642330_0_0_4"/>
    <dbReference type="OMA" id="RISGWRK"/>
    <dbReference type="OrthoDB" id="2133881at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid pMOL30"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="122"/>
            <end position="161"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF12918.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="161" mass="17738" checksum="E56A6CC31856A49A" modified="2005-04-26" version="1">MSIALILKAGEGSIFPNHAKTSDKDPLFLGWMEFPLDEQKKNSLRLDVAVWRKNNSGKDFYSLSIGGIPATLFKEEKQDRPESSPDYAGSFGFGREMRIAGWRKKTKEGDNPYISISVVEKKAAANSERAQTPAAAQEQKAPPRQPAPSQEAAGHPGFDFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-01-04" modified="2022-08-03" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>Q5NUW5</accession>
    <name>Q5NUW5_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Site-specific DNA-methyltransferase (adenine-specific)</fullName>
            <ecNumber evidence="2">2.1.1.72</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Rmet_6243</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4 5">
        <name>pMOL28</name>
    </geneLocation>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="5">Plasmid pMOL28</plasmid>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a 2'-deoxyadenosine in DNA + S-adenosyl-L-methionine = an N(6)-methyl-2'-deoxyadenosine in DNA + H(+) + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:15197"/>
            <dbReference type="Rhea" id="RHEA-COMP:12418"/>
            <dbReference type="Rhea" id="RHEA-COMP:12419"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:90615"/>
            <dbReference type="ChEBI" id="CHEBI:90616"/>
            <dbReference type="EC" id="2.1.1.72"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.1.1.72" evidence="2"/>
    <dbReference type="EMBL" id="CP000355">
        <property type="protein sequence ID" value="ABF13102.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011239941.1">
        <property type="nucleotide sequence ID" value="NC_007972.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_161680.1">
        <property type="nucleotide sequence ID" value="NC_006525.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF13102">
        <property type="protein sequence ID" value="ABF13102"/>
        <property type="gene ID" value="Rmet_6243"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6243"/>
    <dbReference type="HOGENOM" id="CLU_072010_2_0_4"/>
    <dbReference type="OMA" id="HKVFEDF"/>
    <dbReference type="OrthoDB" id="1207120at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid pMOL28"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008170">
        <property type="term" value="F:N-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006306">
        <property type="term" value="P:DNA methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003356">
        <property type="entry name" value="DNA_methylase_A-5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02384">
        <property type="entry name" value="N6_Mtase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="4" id="KW-0614">Plasmid</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N6_Mtase" evidence="3">
        <location>
            <begin position="119"/>
            <end position="223"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF02384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF13102.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="297" mass="33197" checksum="DFA02D79FCA7E702" modified="2005-01-04" version="1">MSRAAKRRFEGSASEPHKVLLSLFKQFGHRYSVHEVFSDFVEIAALSISNSVDRHHAEAREKRYKEIVRRYERVDRERFPQMFGALVQTFEDLVQGWVPNGDGLADVLGQTYMMLELGNDKAGQFFTPYPVSRLMARMNIGDGAPFVQRDGFVTLSEPACGAGGMVIACADALHDAGHNYQQTMHATCIDIDPRCVHMTYVQLALLHIPAIVIHGNALSVEAWSTWFTPAHVVGGWGEKLRFRRRAEAMRELLQSAKRVNETEVGDRAADEAFRADGTPIPAAALRVLVPDDQLALF</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-03-23" modified="2021-06-02" version="33" xmlns="http://uniprot.org/uniprot">
    <accession>D3DXP0</accession>
    <name>D3DXP0_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_6446</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ADC45060.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010792188.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_6446"/>
    <dbReference type="EnsemblBacteria" id="ADC45060">
        <property type="protein sequence ID" value="ADC45060"/>
        <property type="gene ID" value="Rmet_6446"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6446"/>
    <dbReference type="eggNOG" id="ENOG50331K2">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_176022_4_1_4"/>
    <dbReference type="OrthoDB" id="2089632at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021309">
        <property type="entry name" value="DUF2892"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11127">
        <property type="entry name" value="DUF2892"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADC45060.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="67" mass="7471" checksum="8F0238F6C8E28FA0" modified="2010-03-23" version="1">MQINVGNLDRIVRIVIGLILLSLPLWLDSSWRWLGLIGIMPLLTGLAGRCPGYRLLGLSTCPMRKPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-03-23" modified="2020-10-07" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>D3DXX5</accession>
    <name>D3DXX5_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_6538</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ADC45145.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_6538"/>
    <dbReference type="EnsemblBacteria" id="ADC45145">
        <property type="protein sequence ID" value="ADC45145"/>
        <property type="gene ID" value="Rmet_6538"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6538"/>
    <dbReference type="HOGENOM" id="CLU_2864648_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADC45145.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="64" mass="7321" checksum="EA601FA2A07D9CA5" modified="2010-03-23" version="1">MRESSGVSEVGFRWHSWFAWCWLNCGKKEVALRWPLRWGEELIASDAGTPEKQQAPSPFGAWGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-03-23" modified="2020-10-07" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>D3DY55</accession>
    <name>D3DY55_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_6624</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ADC45225.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_6624"/>
    <dbReference type="EnsemblBacteria" id="ADC45225">
        <property type="protein sequence ID" value="ADC45225"/>
        <property type="gene ID" value="Rmet_6624"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6624"/>
    <dbReference type="HOGENOM" id="CLU_2719419_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="53"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADC45225.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="72" mass="7576" checksum="05D85DC3A75C9FFD" modified="2010-03-23" version="1">MQAGLGLDIKVLSKCMLGAPADPCSVPADFTHTSPYLQEDRNSAAIQLLPGCSTLTPQKGRSHERPFPAAPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-03-23" modified="2020-10-07" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>D3DYB8</accession>
    <name>D3DYB8_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_6706</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ADC45288.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_6706"/>
    <dbReference type="EnsemblBacteria" id="ADC45288">
        <property type="protein sequence ID" value="ADC45288"/>
        <property type="gene ID" value="Rmet_6706"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6706"/>
    <dbReference type="HOGENOM" id="CLU_2772950_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADC45288.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="69" mass="7722" checksum="2C49B69D4F23D2D2" modified="2010-03-23" version="1">MSWISDPELRLSAHESPPDNLFAQLLTSLQSGAEEQGRSRHCANLTIVIPVRCRVSRALTQHMLQTHAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="51" xmlns="http://uniprot.org/uniprot">
    <accession>Q1L9U1</accession>
    <name>Q1L9U1_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_6226</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="1 2">
        <name>pMOL28</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid pMOL28</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000355">
        <property type="protein sequence ID" value="ABF13085.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF13085">
        <property type="protein sequence ID" value="ABF13085"/>
        <property type="gene ID" value="Rmet_6226"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6226"/>
    <dbReference type="HOGENOM" id="CLU_1546359_0_0_4"/>
    <dbReference type="OMA" id="YSRYPRW"/>
    <dbReference type="OrthoDB" id="2131911at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid pMOL28"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF13085.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="173" mass="20337" checksum="7B1038A3B5C48BCB" modified="2006-05-30" version="1">MLVAMFHIDFYSRYPRWLFRGGDAAIAGLRGEYVCRSTAGNRVQRVQDRLMHQTIELSSLQKERFTGQYTSWLVQTRDRLTTLSYDTWMDLRYQQNRGASASFSQGAVVFDLIHGVLYEVRGKDRLRRIFRIQLDGDFPYLSFRDPANAIDFPWITFPGVFTQAELMTLRRVY</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-10-07" version="50" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LAE9</accession>
    <name>Q1LAE9_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_6018</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="1 2">
        <name>pMOL30</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid pMOL30</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000354">
        <property type="protein sequence ID" value="ABF12877.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF12877">
        <property type="protein sequence ID" value="ABF12877"/>
        <property type="gene ID" value="Rmet_6018"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6018"/>
    <dbReference type="HOGENOM" id="CLU_979596_0_0_4"/>
    <dbReference type="OMA" id="NKEAFCT"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid pMOL30"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF12877.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="284" mass="30316" checksum="C1DB5C236CDDD6E4" modified="2006-05-30" version="1">MAIVFIEGGAVDRIVVRGNSIQGVHFEEAAYVALAEYQKYLPKARAVPIISTLSQPRASTPTAVAASPAYVAELAPLALGDSAVDASIRAAMSAGVVLNSVDADEIRTAMAKCDDLTRRATRLLRLASDPMRKGNAFPMGVGFTKMTKRASQRIDASVRRAGEASELLRRADSARRYAEGLLAGENTFAARQRATAAQEELQRRVVGELLRWAKGRSILKLGVLRVNRDGAGYPVSFTVSGGNVSAGFGDKVDVVRAFFRGNKEAFCTLVDQLRGSELAGVGQT</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="96" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LAQ1</accession>
    <name>Q1LAQ1_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Transcriptional regulator, LysR family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_5916</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the LysR transcriptional regulatory family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12775.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518125.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5916"/>
    <dbReference type="EnsemblBacteria" id="ABF12775">
        <property type="protein sequence ID" value="ABF12775"/>
        <property type="gene ID" value="Rmet_5916"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5916"/>
    <dbReference type="eggNOG" id="COG0583">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039613_37_2_4"/>
    <dbReference type="OMA" id="RRDDFHW"/>
    <dbReference type="OrthoDB" id="846433at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005119">
        <property type="entry name" value="LysR_subst-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000847">
        <property type="entry name" value="Tscrpt_reg_HTH_LysR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00126">
        <property type="entry name" value="HTH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03466">
        <property type="entry name" value="LysR_substrate"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00039">
        <property type="entry name" value="HTHLYSR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50931">
        <property type="entry name" value="HTH_LYSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH lysR-type" evidence="2">
        <location>
            <begin position="6"/>
            <end position="59"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF12775.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="286" mass="32058" checksum="89B625EFCA407652" modified="2006-05-30" version="1">MHWLGWIRFFEAAARHQNFARAADELHLTHGAISRQIRQLEAELGVSLFERRNRAVFLTEAGRTLQSAATQSMDLLAAAVERIRKPAANTALVLSCEPTIAMRWLIPRLARFSARHPDIPLHLMAAGGAIDFARSGVDVALRRNDFPFDTRWHTREVTPERVGPVCRPDLADHGLDAMPWLHTRTRPDAWQRWARSAGYVLTGSTDVWYEHFYLSLQAASAGLGWAIASELMAYDELSDGRMAAPRGFVADGSAYHLLSPVPFEHDSRRLALFDWLHAEANASSQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LAW6</accession>
    <name>Q1LAW6_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">EPS I polysaccharide export outer membrane protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">epsA</name>
        <name evidence="4" type="ordered locus">Rmet_5851</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="5">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="5">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12710.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008647828.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5851"/>
    <dbReference type="EnsemblBacteria" id="ABF12710">
        <property type="protein sequence ID" value="ABF12710"/>
        <property type="gene ID" value="Rmet_5851"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5851"/>
    <dbReference type="eggNOG" id="COG1596">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG2063">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038343_4_2_4"/>
    <dbReference type="OMA" id="TKGGQFY"/>
    <dbReference type="OrthoDB" id="687725at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015159">
        <property type="term" value="F:polysaccharide transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003715">
        <property type="entry name" value="Poly_export"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019554">
        <property type="entry name" value="Soluble_ligand-bd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02563">
        <property type="entry name" value="Poly_export"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10531">
        <property type="entry name" value="SLBB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0614">Plasmid</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004192976" evidence="2">
        <location>
            <begin position="28"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="domain" description="SLBB" evidence="3">
        <location>
            <begin position="167"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="domain" description="SLBB" evidence="3">
        <location>
            <begin position="253"/>
            <end position="304"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF10531"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF12710.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="362" mass="38836" checksum="0E42605D581B62EC" modified="2006-05-30" version="1">MACAALLLSACAVSPGMQFSSSAPVNAVSPNAMPDVTPITLDLVHEQQAHSKPTNAGVEDLYGTPTPYRIGPGDIISVVVWDHPELVFPTQTYSIGAAFEIPTSSGGSNMPGYVVSPAGDIQFPYAGVMRVAGKTANEVRDEMARILSRVVRDPQMTVRVLGFRSQRVYVDGEVKTPGMLAIDDAPMTLVEALNRAGGVLNLTGDNSRVRVTRGERSWYVNIPALLAKGTDPARILLQSGDIVRVEQREDSKIFVTGEVVKPSSLLMRNGRMTLNEALGDAGGVNPSSANAEQIFVIRRATDGKPKVYHLDGTSPVALAIAESFELEPKDVVYVDARDLVRWSRVMTLLIQPVVGTATIVKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="69" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LAX2</accession>
    <name>Q1LAX2_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Hydrolase_4 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_5845</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12704.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5845"/>
    <dbReference type="EnsemblBacteria" id="ABF12704">
        <property type="protein sequence ID" value="ABF12704"/>
        <property type="gene ID" value="Rmet_5845"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5845"/>
    <dbReference type="eggNOG" id="COG1073">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029181_0_0_4"/>
    <dbReference type="OMA" id="HHVGDGR"/>
    <dbReference type="OrthoDB" id="1698673at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022742">
        <property type="entry name" value="Hydrolase_4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12146">
        <property type="entry name" value="Hydrolase_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="SSF53474"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Hydrolase_4" evidence="2">
        <location>
            <begin position="37"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="595"/>
            <end position="615"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF12704.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="615" mass="66279" checksum="B14B67BC1D0AFB54" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LAZ6</accession>
    <name>Q1LAZ6_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Tyrosine-based site-specific recombinase XerD</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">xerD</name>
        <name evidence="4" type="ordered locus">Rmet_5821</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="5">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="5">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12680.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518031.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5821"/>
    <dbReference type="EnsemblBacteria" id="ABF12680">
        <property type="protein sequence ID" value="ABF12680"/>
        <property type="gene ID" value="Rmet_5821"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5821"/>
    <dbReference type="eggNOG" id="COG4974">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027562_42_1_4"/>
    <dbReference type="OMA" id="WRAVPRI"/>
    <dbReference type="OrthoDB" id="745068at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.443.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044068">
        <property type="entry name" value="CB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022169">
        <property type="entry name" value="DUF3701"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013762">
        <property type="entry name" value="Integrase-like_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010998">
        <property type="entry name" value="Integrase_recombinase_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12482">
        <property type="entry name" value="DUF3701"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51900">
        <property type="entry name" value="CB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0614">Plasmid</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Core-binding (CB)" evidence="3">
        <location>
            <begin position="216"/>
            <end position="330"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF12680.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="526" mass="58120" checksum="2B933B4220DE49A2" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="70" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LB80</accession>
    <name>Q1LB80_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Capsule polysaccharide modification protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">kpsS</name>
        <name evidence="1" type="ordered locus">Rmet_5737</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12596.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011520138.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5737"/>
    <dbReference type="EnsemblBacteria" id="ABF12596">
        <property type="protein sequence ID" value="ABF12596"/>
        <property type="gene ID" value="Rmet_5737"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5737"/>
    <dbReference type="eggNOG" id="COG3562">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_040135_1_0_4"/>
    <dbReference type="OMA" id="WVFEEGY"/>
    <dbReference type="OrthoDB" id="791228at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000271">
        <property type="term" value="P:polysaccharide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015774">
        <property type="term" value="P:polysaccharide transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007833">
        <property type="entry name" value="Capsule_polysaccharide_synth"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05159">
        <property type="entry name" value="Capsule_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF12596.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="461" mass="52505" checksum="443105547F71FCEE" modified="2006-05-30" version="1">MKGHTYAADGLVGRLGVYARVLLLQGPVGPFFAQLRDDLAQQGTEVWKVNLNGGDDLYYYGERVLHFAQPMAAWSDWLRKLVIRARVEAIVVFGSTRRHHRIAAGLAQELGLDFWVFEEGYLRPDFITLERGGVNADSPLARIPLDTIPLLPAPEGLRQYPGAYWKMARHALRYFAAGWAAAGRYPAYRHHKPFRLRELACWIRAAYRKPWYRLRERRLVARLLMPGHPRFYLLALQVYNDSQIRSRSPWRRMEDVIEWSIFSFSKFAPPDSLLVIKHHPMDRGHRDYAAAIATISDRFAVADRIVYVHDPHLPSLLRRSSGVVTVNSTMGVQALHHGVPVMTLGRAFYGHTGMTWQGTLDEFWTLAQPTNRLDWIRWKHYVIHESQVNASFYTGGKAVPSQGMSGIRCATRAFVEMAGRLLRASIHGIQRFGSIANRPVKGAEPLPQSPLPRKSFSDYDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LBE0</accession>
    <name>Q1LBE0_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Heavy metal cation tricomponent efflux outer membrane porin NimC (CzcC-like)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">nimC</name>
        <name evidence="3" type="ordered locus">Rmet_5677</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the outer membrane factor (OMF) (TC 1.B.17) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12536.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011520082.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5677"/>
    <dbReference type="EnsemblBacteria" id="ABF12536">
        <property type="protein sequence ID" value="ABF12536"/>
        <property type="gene ID" value="Rmet_5677"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5677"/>
    <dbReference type="eggNOG" id="COG1538">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_055090_0_0_4"/>
    <dbReference type="OMA" id="HSHEYLL"/>
    <dbReference type="OrthoDB" id="1592039at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015562">
        <property type="term" value="F:efflux transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003423">
        <property type="entry name" value="OMP_efflux"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02321">
        <property type="entry name" value="OEP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193292" evidence="2">
        <location>
            <begin position="26"/>
            <end position="419"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF12536.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="419" mass="44990" checksum="B4FB51DA28ADDD26" modified="2006-05-30" version="1">MKPAMRNIVLAMIGAALLSNGAVFAQDAQSYLPAEVAVREAVARAPDVLTAEARRDATTARAAGTRAGTAETVVRVIGQGRQVREPSERYPEGQIAIERPLRLWGKAEADGVLADLATEAGNLSVKDAKHETSRQILSLWIAAIRAGQARMAAEDNARAAADLAAMTTRRVQVGDAARLDAELAAAELARTRATVATAQATESASLAELRARFPMLGQPVQAPVRIALPPPPQEPVEQLRGIYVQDSHEYLLAMAEEAHALQQAKRSDLDRKPDPTVGMFVTVERGGAERILGVSVSMPLGSAYRRSNAVAAAADAEAAARKRLGIEQRLGAEFDVLYRNLSGKRAAAAAQTEAATLQRTASDRATRAYRAGESGLQELIVVRRNLADAMLAERLANVDVLESDSRLKLDLHRMWDFDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LBT9</accession>
    <name>Q1LBT9_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">AMP-dependent synthetase and ligase putative o-succinylbenzoate-CoA ligase</fullName>
            <ecNumber evidence="3">6.2.1.26</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_5528</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EC" id="6.2.1.26" evidence="3"/>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12387.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519931.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5528"/>
    <dbReference type="EnsemblBacteria" id="ABF12387">
        <property type="protein sequence ID" value="ABF12387"/>
        <property type="gene ID" value="Rmet_5528"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5528"/>
    <dbReference type="eggNOG" id="COG0318">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000022_59_0_4"/>
    <dbReference type="OMA" id="EVSWATI"/>
    <dbReference type="OrthoDB" id="377638at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008756">
        <property type="term" value="F:o-succinylbenzoate-CoA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025110">
        <property type="entry name" value="AMP-bd_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045851">
        <property type="entry name" value="AMP-bd_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020845">
        <property type="entry name" value="AMP-binding_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_Synth/Lig"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13193">
        <property type="entry name" value="AMP-binding_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00455">
        <property type="entry name" value="AMP_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0436">Ligase</keyword>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AMP-binding" evidence="1">
        <location>
            <begin position="16"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="domain" description="AMP-binding_C" evidence="2">
        <location>
            <begin position="416"/>
            <end position="491"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF12387.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="509" mass="56404" checksum="CC4F0F21D3FE66A8" modified="2006-05-30" version="1">MSEATIYLDGRAKARDEVLQQAMRAATGFDKMGIREGDTVASLLRNDFSFFEVQQAAAAVGAYSVPLNWHGKTEELTYILNDAKPKVLVAHADLLEPLRPHIPQGLQVLVVPTPPEVQSRYGISDALATPKAGDIGWPEWCQTFELWTQPPKRGRATMIYTSGTTGHPKGVKRDPATPEQAKAYVDIIERVYGLTPGVRALITGPLYHASPNAYGRQAITAADVLVLQSKFDPEETLAAIEKYRITNAVMVPTMFIRILKLPKEVRERYDVSSLKWVTHTGAPCPREVKQELMEWWGPVVYETYGGTEVGTATLATPDDWLNHPGSVGVPTPGTQIAFFGEDGKPVEDGTPGEIYMRVPAYADFTYLNHEEKRKSVERDGLISVGDVGYLKEGRLYLCDRRSDMVISGGTNIYPAEIEMVLTQCPGVHDCAVFGIPDEDFGESLAAAVELMPGAELSASDIQKYLESHLAKYKVPRRIDFHASLPREDSGKIFKRRLRDPFWQAVGRKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LC03</accession>
    <name>Q1LC03_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_5464</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12323.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF12323">
        <property type="protein sequence ID" value="ABF12323"/>
        <property type="gene ID" value="Rmet_5464"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5464"/>
    <dbReference type="HOGENOM" id="CLU_2261484_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="33"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="47"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF12323.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="103" mass="11158" checksum="D0A031E125DEAAFA" modified="2006-05-30" version="1">MSLAAFSFVDGPMTEGYLRPFFDSGHSPNRHTCSLLGTPIGSRSSSTTKRSDERSAAASANDERGKQELLAPAELNVDEPREVTARELSFSLWWAIQEAPSGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="60" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LC16</accession>
    <name>Q1LC16_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_5451</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12310.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519857.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5451"/>
    <dbReference type="EnsemblBacteria" id="ABF12310">
        <property type="protein sequence ID" value="ABF12310"/>
        <property type="gene ID" value="Rmet_5451"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5451"/>
    <dbReference type="eggNOG" id="COG3826">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_073550_0_0_4"/>
    <dbReference type="OMA" id="FTVNQRP"/>
    <dbReference type="OrthoDB" id="1025956at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018655">
        <property type="entry name" value="DUF2086"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09859">
        <property type="entry name" value="Oxygenase-NA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF12310.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="215" mass="24203" checksum="577A0361F9F6DE84" modified="2006-05-30" version="1">MTPEAYDWMNIGISLDAYGNAVLPGLLTVEQCDGLAALYPNEKGYRTRIVMARHGFGRGEYKYFAYPLPPLLEQLRHALYRRLAPIANRWNLQLGIDVQYPDDLVAFLRRCHQAGQTRPTPLILQYGEGDYNCLHQDLYGEHVFPLQVAILLSEPGKDFAGGEFVMTERSTKGQRADVVSLRKGDALVFTVNHRPAMGRSGARKVSMRHGVSRIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="70" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LC57</accession>
    <name>Q1LC57_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative membrane protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_5410</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12269.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519817.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5410"/>
    <dbReference type="EnsemblBacteria" id="ABF12269">
        <property type="protein sequence ID" value="ABF12269"/>
        <property type="gene ID" value="Rmet_5410"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5410"/>
    <dbReference type="eggNOG" id="COG0845">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_049021_0_0_4"/>
    <dbReference type="OMA" id="ERIMMIA"/>
    <dbReference type="OrthoDB" id="977177at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039562">
        <property type="entry name" value="MFP_biotin_lipoyl_2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13533">
        <property type="entry name" value="Biotin_lipoyl_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Biotin_lipoyl_2" evidence="1">
        <location>
            <begin position="217"/>
            <end position="259"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF12269.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="449" mass="49786" checksum="7FB794E76405F403" modified="2006-05-30" version="1">MQMPTQSDVVLLLQLAELESLARQADSVQQLTFHIANDAHPLLRYRQALVFDVRPPAWHLLNISGLVSIDEQSPYVVWLGHARKWLRERLESGEPQWITQEDAALASDVVRSGWEEWWPNGAWALPLRSRAGQVQGWVVFLLDEVPGAGAGIAMSRLTQAWGYHWEMLARRERDVSWLWGSRTRRIAIAAAVLILLMPVRQSALAPAEVTSLDLQMVSAPIEGVVKEVHVRPNQTVKAGQPLFSLDDTTLSHRLEVASQAVAVADAELLAARQNAFRSDDSKAQLTTLQSRAEERRAELESIRSQLQRQTVVAPRDGVAVFADINDWLGKPVVTGERIMQLANPDRPAMLIHLPVADALTLDVGAPVSLFLTVRPLQPLSGKLIETSYQAALTPDGVPSYRLRADIENGDPQHARIGLKGTARIKGGWSVLAYEILRRPLATLRAWTGI</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-12-12" modified="2022-08-03" version="99" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LC89</accession>
    <name>HMUV_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Hemin import ATP-binding protein HmuV</fullName>
            <ecNumber evidence="1">7.6.2.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">hmuV</name>
        <name type="ordered locus">Rmet_5378</name>
    </gene>
    <organism>
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid">
        <name>megaplasmid</name>
    </geneLocation>
    <reference key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Part of the ABC transporter complex HmuTUV involved in hemin import. Responsible for energy coupling to the transport system.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">The complex is composed of two ATP-binding proteins (HmuV), two transmembrane proteins (HmuU) and a solute-binding protein (HmuT).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell inner membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the ABC transporter superfamily. Heme (hemin) importer (TC 3.A.1.14.5) family.</text>
    </comment>
    <dbReference type="EC" id="7.6.2.-" evidence="1"/>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12237.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519784.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q1LC89"/>
    <dbReference type="SMR" id="Q1LC89"/>
    <dbReference type="STRING" id="266264.Rmet_5378"/>
    <dbReference type="EnsemblBacteria" id="ABF12237">
        <property type="protein sequence ID" value="ABF12237"/>
        <property type="gene ID" value="Rmet_5378"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5378"/>
    <dbReference type="eggNOG" id="COG4559">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_11_4"/>
    <dbReference type="OMA" id="HHADRVW"/>
    <dbReference type="OrthoDB" id="1752365at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51261">
        <property type="entry name" value="HMUV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0997">Cell inner membrane</keyword>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0614">Plasmid</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-1278">Translocase</keyword>
    <keyword id="KW-0813">Transport</keyword>
    <feature type="chain" id="PRO_0000269614" description="Hemin import ATP-binding protein HmuV">
        <location>
            <begin position="1"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="1">
        <location>
            <begin position="2"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="34"/>
            <end position="41"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01718"/>
        </source>
    </evidence>
    <sequence length="261" mass="27585" checksum="AEF048EFA86E2810" modified="2006-05-30" version="1">MLIARNLSCSRGRRQILSGIDLTLPAGEMVGVLGANGAGKSTLLGTLAGELPGDDAAVSLCNRPLTDWPVDELARSRAVLPQSPGLGFDLGVTEVVAMGGYPFPEMGKHTLDTLLDRALALADVAHLATRSYQSLSGGEQQRVQFARALVQVLACRTADSYRALLLDEPISSLDPRHQLQLLGTARNLCHTDALAVLVVLHDVNLAARWCDRLVLLADGRTVASGTPADVLTPPNLATVYGIPATVMESPVHRGVPMVVFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-10-07" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LCD4</accession>
    <name>Q1LCD4_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_5333</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12192.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF12192">
        <property type="protein sequence ID" value="ABF12192"/>
        <property type="gene ID" value="Rmet_5333"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5333"/>
    <dbReference type="HOGENOM" id="CLU_1884066_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF12192.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="135" mass="14996" checksum="A83DE81EF0EAB161" modified="2006-05-30" version="1">MEIMTPHGSRGWHVAGHGTCVPWRATGVRIADASYQSVRQNRMAASAYSRSDLGASVRPAWPSDVEFVVEVEVFDCVWARRNGETRRSVSAEVRVVDARNLRISYSFAVLPTMAEQHGACRDRRVRVARGGLGRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LCH3</accession>
    <name>Q1LCH3_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Extra-cytoplasmic solute receptor protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">bug</name>
        <name evidence="4" type="ordered locus">Rmet_5294</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="5">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="5">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Periplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the UPF0065 (bug) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12153.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519698.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5294"/>
    <dbReference type="EnsemblBacteria" id="ABF12153">
        <property type="protein sequence ID" value="ABF12153"/>
        <property type="gene ID" value="Rmet_5294"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5294"/>
    <dbReference type="eggNOG" id="COG3181">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045683_0_2_4"/>
    <dbReference type="OrthoDB" id="447734at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042597">
        <property type="term" value="C:periplasmic space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005064">
        <property type="entry name" value="BUG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042100">
        <property type="entry name" value="Bug_dom1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42928">
        <property type="entry name" value="PTHR42928"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03401">
        <property type="entry name" value="TctC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017082">
        <property type="entry name" value="YflP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-0675">Receptor</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193004" evidence="3">
        <location>
            <begin position="31"/>
            <end position="330"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF12153.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="330" mass="35228" checksum="DABEB2923D71D475" modified="2006-05-30" version="1">MTWITHGARQAMALALVLGSALPAMEAARAADWPTRPVRILVGAPPGGTADIVARLVARELQASLGQPVIVDYKAGAGGTIAVQTMLASPRDGYTFLLIQKGIASEVPHAIKVSYDPFKDIVPIAQLTQYGLVLVGNPNLPAKDLGELVRYVKSKPGKFDYANFGVGTRGQTMGVQFNRVAGLDAGTVSYKGSPAALQDVMGGQVPLMFDGPATSLPFIKAGKLRAYAVASPRRMAALPDVPTFTESGYPDLQDVSWMGIWSAQGVPPAVMTKMRDATLKVTQSPTLRPRLEDMGMEPGSPATTDELLKDVRESYERQGALLRSIHFKPD</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LD46</accession>
    <name>Q1LD46_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative acetyltransferase, GNAT family</fullName>
            <ecNumber evidence="2">2.3.1.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_5068</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EC" id="2.3.1.-" evidence="2"/>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11930.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5068"/>
    <dbReference type="EnsemblBacteria" id="ABF11930">
        <property type="protein sequence ID" value="ABF11930"/>
        <property type="gene ID" value="Rmet_5068"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5068"/>
    <dbReference type="eggNOG" id="COG1247">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013985_4_4_4"/>
    <dbReference type="OMA" id="CAAIYAP"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008080">
        <property type="term" value="F:N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="SSF55729"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="1">
        <location>
            <begin position="1"/>
            <end position="154"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF11930.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="164" mass="18361" checksum="674719AD44545166" modified="2006-05-30" version="1">MEAVCAIYNDAVANTTAIWNDVLVDVDNRKDWLAARRKAGFPVLVAQDEAGAVVGYASFGDWRAFDGYRHTVEHSVYIRNDQRGRGIAGLMMKALIERARDLNKHIMVGAIEAGNASSIRLHEKLGFEQVGLMKEVGMKFGKWLDLVFMQLNIDHRSTPDVAQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-10-07" version="56" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LD50</accession>
    <name>Q1LD50_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_5064</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11926.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5064"/>
    <dbReference type="EnsemblBacteria" id="ABF11926">
        <property type="protein sequence ID" value="ABF11926"/>
        <property type="gene ID" value="Rmet_5064"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5064"/>
    <dbReference type="eggNOG" id="COG2913">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1812552_0_0_4"/>
    <dbReference type="OMA" id="FFEFDEN"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="33"/>
            <end position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF11926.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="152" mass="16347" checksum="B76A2D962E7ECE5E" modified="2006-05-30" version="1">MDWAYCPARLGPRRPARLQSRPRKQGGDDMNRFYAMVAVCGLMAACVSTGIDVKPEQMQSFILGFSTLDDVTGQLGNPTSQATTSDGAIVLVYAYATSKPHPESFLPFIGPLFAGGDILSSTVLFEFDQNGVLRSERRTTSSGATGLTVLPH</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LD70</accession>
    <name>Q1LD70_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Mechanosensitive ion channel family protein (MscS-type)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Rmet_5044</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="6">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="6">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11906.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519457.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5044"/>
    <dbReference type="EnsemblBacteria" id="ABF11906">
        <property type="protein sequence ID" value="ABF11906"/>
        <property type="gene ID" value="Rmet_5044"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5044"/>
    <dbReference type="eggNOG" id="COG0668">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021080_0_0_4"/>
    <dbReference type="OMA" id="MPVSYFT"/>
    <dbReference type="OrthoDB" id="1597438at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010920">
        <property type="entry name" value="LSM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006685">
        <property type="entry name" value="MscS_channel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023408">
        <property type="entry name" value="MscS_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00924">
        <property type="entry name" value="MS_channel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50182">
        <property type="entry name" value="SSF50182"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 4" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0614">Plasmid</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2 4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="12"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="49"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="90"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="133"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="160"/>
            <end position="178"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABF11906.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="357" mass="39873" checksum="ECC802975CC7E82A" modified="2006-05-30" version="1">MNLPLLFDHPWFGTWTAALVAVVVVLLAHRIGSLLLLRITRSAQILHPMVVKARVPSKAVLLLLALQTVWQAAPDELRWIDNVRHVNGLLLIAAMTWLVARVIAGFAQGVLDRHPVDVEDNMNARRIHTQTRVLSRIAMALVVVIGAAMLLMTFPGARQVGASLLASAGVVGLVAGLAGKTVFSNMIAGLQLALTQPIRLDDVLIVEGEWGRVEEITATYVVLRIWDERRLIIPLQYFIEKPFQNWTRNNSQLLGSVFFHVDYGMPLAPLRKELERIVNNAPQWDRRFFNLVVTDATERTMQIRVLCTAASSGLAWDLRCVVREGLIDFMQREYPQFLPRMRIDGEAGRTGGNGAQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="70" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LDL3</accession>
    <name>Q1LDL3_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Putative regulator containing a HipA-like domain</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Rmet_4901</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="6">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="6">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the HipA Ser/Thr kinase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11763.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519323.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4901"/>
    <dbReference type="EnsemblBacteria" id="ABF11763">
        <property type="protein sequence ID" value="ABF11763"/>
        <property type="gene ID" value="Rmet_4901"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4901"/>
    <dbReference type="eggNOG" id="COG3550">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030167_3_0_4"/>
    <dbReference type="OMA" id="HMEEFAQ"/>
    <dbReference type="OrthoDB" id="1293681at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012893">
        <property type="entry name" value="HipA-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017508">
        <property type="entry name" value="HipA_N1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13657">
        <property type="entry name" value="Couple_hipA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07804">
        <property type="entry name" value="HipA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03071">
        <property type="entry name" value="couple_hipA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0614">Plasmid</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Couple_hipA" evidence="4">
        <location>
            <begin position="18"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="domain" description="HipA_C" evidence="3">
        <location>
            <begin position="163"/>
            <end position="405"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010164"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF07804"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF13657"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABF11763.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="433" mass="48482" checksum="5355C8F1182CB3A0" modified="2006-05-30" version="1">MTTSIRYLRLFLYTATGRRGIGYLSQYGDILRISFDRDYIEDENRPTLSLGYVGETEAATRAILTALRDVRVVRTDGHWPVFFQNLLPEGHNRERLASERGCSTEDEFELLAAAGHDLPGAVEVEPVPPAEGVPQIVRNWHTALGLDVLEPGFVEDPVEDAAAIPGVVTKFSAIQQGRRYVVKRHGEAGDFILKLPSTRHPDLVENEFTGYQLCRALGLDCAEATVISREEAELPEQVPFQRILAVKRFDRAPGGHRVHMEEFAQILGYAPRNKYGRALALDYGNMLRVLDRLSARPAPDVQEFIKRFVAFLLMGNTDAHLKNWAVRYPDGRAPVLSPLYDPVCVTAFFDDVPVTDYGINRAIDKTLRAYTFDDLDAMVRSAGLLRRARLLSIARETVRQAQADWPRILEDAPEGVRRAVSERLAGGVALTRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="97" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LDM7</accession>
    <name>Q1LDM7_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative transcriptional regulator, TetR family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_4887</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11749.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519308.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4887"/>
    <dbReference type="EnsemblBacteria" id="ABF11749">
        <property type="protein sequence ID" value="ABF11749"/>
        <property type="gene ID" value="Rmet_4887"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4887"/>
    <dbReference type="eggNOG" id="COG1309">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069356_23_1_4"/>
    <dbReference type="OMA" id="WFGQPDF"/>
    <dbReference type="OrthoDB" id="1826886at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001647">
        <property type="entry name" value="HTH_TetR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036271">
        <property type="entry name" value="Tet_transcr_reg_TetR-rel_C_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00440">
        <property type="entry name" value="TetR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00455">
        <property type="entry name" value="HTHTETR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48498">
        <property type="entry name" value="SSF48498"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50977">
        <property type="entry name" value="HTH_TETR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH tetR-type" evidence="2">
        <location>
            <begin position="4"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="H-T-H motif" evidence="1">
        <location>
            <begin position="27"/>
            <end position="46"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF11749.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="191" mass="21015" checksum="4048AB99D4EAC5F8" modified="2006-05-30" version="1">MPTPSARDRILDTAARLFYRDGYRGTGIDRIIAESGVAKMSLYRHFPSKNDLIVAYLESRHDYWMGWFRDAVTRRLAARGNLDIMADALGEWFATEDFRGCSFINMIAEAGGGAGDDPRLLAQAVAHKASLEDFVTGIAHDLGLSSPREVAEEAMLCIEGMIIRYQTTHASSVIDAGRRTLGRLHAAHSAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="55" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LDT1</accession>
    <name>Q1LDT1_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_4833</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11695.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008641381.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4833"/>
    <dbReference type="EnsemblBacteria" id="ABF11695">
        <property type="protein sequence ID" value="ABF11695"/>
        <property type="gene ID" value="Rmet_4833"/>
    </dbReference>
    <dbReference type="GeneID" id="60821579"/>
    <dbReference type="KEGG" id="rme:Rmet_4833"/>
    <dbReference type="HOGENOM" id="CLU_2247862_0_0_4"/>
    <dbReference type="OMA" id="MTHEILS"/>
    <dbReference type="OrthoDB" id="2067099at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF11695.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="104" mass="11408" checksum="6A723B9230A6F3E9" modified="2006-05-30" version="1">MKRDAVAVTETTPEGLAALIYHIAHSASQGQLDPEFVRKLGKRVDKELEAMEAGGKMNATDKKQLLHAVQTLHTTTDVEEGARLTRALERLRDHDARAALPPAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LDZ2</accession>
    <name>Q1LDZ2_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">D-aminoacylase (N-acyl-D-amino-acid deacylase)</fullName>
            <ecNumber evidence="2">3.5.1.81</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_4772</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EC" id="3.5.1.81" evidence="2"/>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11634.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519204.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4772"/>
    <dbReference type="EnsemblBacteria" id="ABF11634">
        <property type="protein sequence ID" value="ABF11634"/>
        <property type="gene ID" value="Rmet_4772"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4772"/>
    <dbReference type="eggNOG" id="COG3653">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016107_2_0_4"/>
    <dbReference type="OMA" id="HATMNFG"/>
    <dbReference type="OrthoDB" id="1319925at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047420">
        <property type="term" value="F:N-acyl-D-amino-acid deacylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013108">
        <property type="entry name" value="Amidohydro_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023100">
        <property type="entry name" value="D-aminoacylase_insert_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011059">
        <property type="entry name" value="Metal-dep_hydrolase_composite"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07969">
        <property type="entry name" value="Amidohydro_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51338">
        <property type="entry name" value="SSF51338"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="SSF51556"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Amidohydro_3" evidence="1">
        <location>
            <begin position="55"/>
            <end position="463"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF07969"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF11634.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="487" mass="52219" checksum="334B9972BC5A2AFD" modified="2006-05-30" version="1">MPHLFDTLIRCVLLLDGSGAAARLADVAIRDGRIARIDEPGAIDPDSASNVVEGHGLVLSPGFIDVHTHDDTNVVREPAMTPKISQGVTTVVVGNCGISAAPVTLAGDPPDPMNLLGRADAFRYPDFKTYVAAVNAAKPAVNVAALVGHTALRSNHMDRLDRPATEAETAAMRGQLQEALDHGALGLSTGLAYANAFHAPTDEVLALAEPLAKAGAIYATHLRSEFAEILDAMDEAFRIGRHARVPVVISHLKCAGVSNWGRSGEVLAALEGAERWQPVGCDCYPYTASSSTLDLKQVTNDFDIQVTWSEPHPTMGGRLLADIAAEWNVDLLEAARRLQPAGAVYHCMEDADVDRILSHPATVVGSDGLPNDPLPHPRLWGAFPRVLGHYARDRELFPLAVAVNKMTGMSAERFGLTGRGFVREGYWADLVLFDAATIRDAASFTDPQQPAEGIAAVWVNGELTWRQRAATGVRAGRFLPRGTQQQH</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LE46</accession>
    <name>Q1LE46_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative permeases of the drug/metabolite transporter (DMT) superfamily</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_4718</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11580.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519152.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4718"/>
    <dbReference type="EnsemblBacteria" id="ABF11580">
        <property type="protein sequence ID" value="ABF11580"/>
        <property type="gene ID" value="Rmet_4718"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4718"/>
    <dbReference type="eggNOG" id="COG0697">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033863_10_0_4"/>
    <dbReference type="OMA" id="RREWPRH"/>
    <dbReference type="OrthoDB" id="1425840at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000620">
        <property type="entry name" value="EamA_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00892">
        <property type="entry name" value="EamA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="36"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="70"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="96"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="128"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="151"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="193"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="218"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="250"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="275"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="domain" description="EamA" evidence="2">
        <location>
            <begin position="8"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="domain" description="EamA" evidence="2">
        <location>
            <begin position="154"/>
            <end position="289"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF11580.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="302" mass="31393" checksum="FA0B3CF91755B37C" modified="2006-05-30" version="1">MSRPFPAWLPAMAFVLIWSTGFIVGKAVVPVADANLFLLARFTLAALMFALAAMASGVTWPALKQVPRHLLAGALMQGIYLCAGYGAVAHGLSPSIMALLGALQPLLTALLAIPLLKEQPSSRTWRGLGLGALGVGLVVLPAMQAGTPQTVSPWIILTGVLAILSITMGTLLQKTSIARADIRASSAWQNVGAMLVAGVLVCATGGIGALRWETGPTLWASLAWAAFVLSGIGTWLLLSLVRRGQAANAAALMFLAPPLAAVQAALLFGDRLAPLQWLGMAVAGIGVWLCQTQGNPRHAQAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="58" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LEF3</accession>
    <name>Q1LEF3_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_4611</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11473.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519048.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4611"/>
    <dbReference type="EnsemblBacteria" id="ABF11473">
        <property type="protein sequence ID" value="ABF11473"/>
        <property type="gene ID" value="Rmet_4611"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4611"/>
    <dbReference type="eggNOG" id="ENOG5032S8H">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_142305_0_0_4"/>
    <dbReference type="OMA" id="IAYAPEF"/>
    <dbReference type="OrthoDB" id="1787530at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF11473.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="116" mass="12404" checksum="920F008D7FD2263E" modified="2006-05-30" version="1">MRVPLRRLAHSRSGDKGNTSNTAVIAYAPEFYPHIKQQLTADAFKAHYGALITGLVTRFDVDGLQVLNFVAEGALGGGVSRSLAIDNYGKALASAVLRFEVEIPDALAPLLRGPAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="98" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LEG4</accession>
    <name>Q1LEG4_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Sulphate transporter (Tn6050)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">sulP</name>
        <name evidence="3" type="ordered locus">Rmet_4472</name>
        <name evidence="4" type="ordered locus">Rmet_4600</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid</name>
    </geneLocation>
    <geneLocation type="plasmid" evidence="5">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="submission" date="2006-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of the chromosome of Ralstonia metallidurans CH34.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Copeland A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Barry K."/>
                <person name="Detter J.C."/>
                <person name="Glavina del Rio T."/>
                <person name="Hammon N."/>
                <person name="Israni S."/>
                <person name="Dalin E."/>
                <person name="Tice H."/>
                <person name="Martinez M."/>
                <person name="Goltsman E."/>
                <person name="Pitluck S."/>
                <person name="Schmutz J."/>
                <person name="Larimer F."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Kim E."/>
                <person name="Mergeay M."/>
                <person name="Benotmane M.A."/>
                <person name="Vallaeys T."/>
                <person name="Michaux A."/>
                <person name="Monchy S."/>
                <person name="Dunn J."/>
                <person name="McCorkle S."/>
                <person name="Taghavi S."/>
                <person name="van der Lelie D."/>
                <person name="Richardson P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">CH34</strain>
            <plasmid evidence="4">megaplasmid</plasmid>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="5">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2010-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Benotmane R."/>
                <person name="Lapidus A."/>
                <person name="McCorkle S."/>
                <person name="Monchy S."/>
                <person name="Taghavi S."/>
                <person name="van der Lelie N."/>
                <person name="Dunn J."/>
                <person name="Leys N."/>
                <person name="Mergeay M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">CH34</strain>
            <plasmid evidence="4">megaplasmid</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11337.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11462.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518938.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4472"/>
    <dbReference type="EnsemblBacteria" id="ABF11337">
        <property type="protein sequence ID" value="ABF11337"/>
        <property type="gene ID" value="Rmet_4472"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF11462">
        <property type="protein sequence ID" value="ABF11462"/>
        <property type="gene ID" value="Rmet_4600"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4472"/>
    <dbReference type="KEGG" id="rme:Rmet_4600"/>
    <dbReference type="eggNOG" id="COG0659">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003182_7_0_4"/>
    <dbReference type="OMA" id="GCALVGQ"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008271">
        <property type="term" value="F:secondary active sulfate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018045">
        <property type="entry name" value="S04_transporter_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011547">
        <property type="entry name" value="SLC26A/SulP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002645">
        <property type="entry name" value="STAS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036513">
        <property type="entry name" value="STAS_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01740">
        <property type="entry name" value="STAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00916">
        <property type="entry name" value="Sulfate_transp"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="SSF52091"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01130">
        <property type="entry name" value="SLC26A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50801">
        <property type="entry name" value="STAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
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    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="49"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="88"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="114"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="146"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="169"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="218"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="260"/>
            <end position="278"/>
        </location>
    </feature>
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        <location>
            <begin position="290"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="315"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="349"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="domain" description="STAS" evidence="2">
        <location>
            <begin position="389"/>
            <end position="492"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF11337.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF11462.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="492" mass="52559" checksum="1D4B532EF39081D1" modified="2006-05-30" version="1">MMKSLRQDWLSNVRNDLLAGIVVALALIPEAIAFSIIAGVDPKVGLYASFSIAAIIAFAGGRPGMVSAATGAMALLMVTLVKDHGLQYLLAATVLTGVLQILAGWLKLGALMRFVSRSVITGFVNALAILIFMAQLPELTNVTWHVYAMTAAGLGIIYGFPYITKAVPSPLVAIVVLTGVAIFLGLDIRTVGDMGKLPDSLPVFLLPDVPLNLETLKIIFPVSATLAVVGLLESMMTASIVDDLTDSNSNKNRECVGQGVANIATGFLGGMAGCAMIGQSVINVKSGGRGRLSTLAAGVFLLLMVVFIGDWVARIPMAALVAVMIMVSIGTFNWASIRNLREHPKSSSMVMLATVVVTVGTHDLAKGVLIGVLLSGFFFAHKVGQILRVTSRTEDEGRVRTYTITGQVFFASADRFINTFDYKEVIEKVRIDVSRAHFWDITAVSALDKVVIKFRREATEVEVIGLNEASATMVDKFAVHDKDGAEDMLMGH</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LEN3</accession>
    <name>Q1LEN3_CUPMC</name>
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        <submittedName>
            <fullName evidence="2">Nonspecific acid phosphatase napD-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_4528</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
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        </lineage>
    </organism>
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    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
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            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11393.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4528"/>
    <dbReference type="EnsemblBacteria" id="ABF11393">
        <property type="protein sequence ID" value="ABF11393"/>
        <property type="gene ID" value="Rmet_4528"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4528"/>
    <dbReference type="eggNOG" id="COG0560">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_052514_0_0_4"/>
    <dbReference type="OMA" id="GIDFMRV"/>
    <dbReference type="OrthoDB" id="1472357at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="SSF56784"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193128" evidence="1">
        <location>
            <begin position="27"/>
            <end position="358"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF11393.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="358" mass="39778" checksum="4B4E15FCC461F7D9" modified="2006-05-30" version="1">MEHARAPWLRRWINTAVAALVIGATATTLGGCASSAPQSGGTQESAQTAVNAEAALPSWRDGATRKTIIKFVADVTQQGSPTYVPPAERIAVFDNDGTLWSEQPLYFQFFFLLDQVKAAAPSHPEWKNNPAFKALMANDMAGLMREEKRLLPLITAANSGMTVDEYDRTIRAWIDTARHPKLNRRYTDLVYQPQLELLSYLRANGFKTYIVSGGTIEFMRPWTERVYGIPPEQVVGSTQTITYDVRDGKPVLMRGPKLDFIDDGPGKPVGIYRQIGRQPILAVGNSDGDLQMLQYTMAGTGARLAMLVHHDDAQREFAYDRQSKIGKLDKALDEATTRGWTVISMQKDWAQIYPAAKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="52" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LER7</accession>
    <name>Q1LER7_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_4494</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11359.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF11359">
        <property type="protein sequence ID" value="ABF11359"/>
        <property type="gene ID" value="Rmet_4494"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4494"/>
    <dbReference type="eggNOG" id="ENOG5034AAQ">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2247863_0_0_4"/>
    <dbReference type="OMA" id="IFVINRK"/>
    <dbReference type="OrthoDB" id="1798036at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF11359.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="103" mass="11264" checksum="0312A7D48EAE10A2" modified="2006-05-30" version="1">MKFKAQIFVINRKDWSFDGKSGTTYSAQMLITSAREEDGKMVEDVFVARLKVPEHMKNTQPGEYITELVPFADGNGNLDFRVVALVPFGRPAANGKAPASAAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="64" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LF31</accession>
    <name>Q1LF31_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_4380</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11245.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518862.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4380"/>
    <dbReference type="EnsemblBacteria" id="ABF11245">
        <property type="protein sequence ID" value="ABF11245"/>
        <property type="gene ID" value="Rmet_4380"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4380"/>
    <dbReference type="eggNOG" id="COG4648">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_108379_0_0_4"/>
    <dbReference type="OMA" id="HFAPWQF"/>
    <dbReference type="OrthoDB" id="1580783at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="26"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="51"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="74"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="125"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="149"/>
            <end position="171"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF11245.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="185" mass="20422" checksum="81595EC0518B5719" modified="2006-05-30" version="1">MAWRKWGLGLLTMAYPLVIYFGLQHYSPRVMALALVVLALLRAATSRQTGWRVTALLAAALAIAVLASDHELPLKLYPVLVNVAMLAVFGWSLRHPPTLIERLARLREPDLPPSGVAYTRRVTQAWCVFFVVNGTIAAITALAGSDRAWAIYNGGIAYGLIGAMFAVEWLVRQRVKRRHAEGHHG</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="96" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LF50</accession>
    <name>Q1LF50_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Formyl-CoA:oxalate CoA-transferase</fullName>
            <shortName evidence="1">FCOCT</shortName>
            <ecNumber evidence="1">2.8.3.16</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Formyl-coenzyme A transferase</fullName>
            <shortName evidence="1">Formyl-CoA transferase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1 2" type="primary">frc</name>
        <name evidence="2" type="ordered locus">Rmet_4361</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in the catabolism of oxalate and in the adapatation to low pH via the induction of the oxalate-dependent acid tolerance response (ATR). Catalyzes the transfer of the CoA moiety from formyl-CoA to oxalate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>formyl-CoA + oxalate = formate + oxalyl-CoA</text>
            <dbReference type="Rhea" id="RHEA:16545"/>
            <dbReference type="ChEBI" id="CHEBI:15740"/>
            <dbReference type="ChEBI" id="CHEBI:30623"/>
            <dbReference type="ChEBI" id="CHEBI:57376"/>
            <dbReference type="ChEBI" id="CHEBI:57388"/>
            <dbReference type="EC" id="2.8.3.16"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Metabolic intermediate degradation; oxalate degradation; CO(2) and formate from oxalate: step 1/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the CoA-transferase III family. Frc subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="1">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.8.3.16" evidence="1"/>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11226.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518843.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4361"/>
    <dbReference type="EnsemblBacteria" id="ABF11226">
        <property type="protein sequence ID" value="ABF11226"/>
        <property type="gene ID" value="Rmet_4361"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4361"/>
    <dbReference type="eggNOG" id="COG1804">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033975_2_1_4"/>
    <dbReference type="OMA" id="YVIIQPQ"/>
    <dbReference type="OrthoDB" id="969868at2"/>
    <dbReference type="UniPathway" id="UPA00540">
        <property type="reaction ID" value="UER00598"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033608">
        <property type="term" value="F:formyl-CoA transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033611">
        <property type="term" value="P:oxalate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1540.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10540">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00742">
        <property type="entry name" value="Formyl_CoA_transfer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003673">
        <property type="entry name" value="CoA-Trfase_fam_III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044855">
        <property type="entry name" value="CoA-Trfase_III_dom3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023606">
        <property type="entry name" value="CoA-Trfase_III_dom_1_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017659">
        <property type="entry name" value="Formyl_CoA_transfer"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02515">
        <property type="entry name" value="CoA_transf_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89796">
        <property type="entry name" value="SSF89796"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03253">
        <property type="entry name" value="oxalate_frc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Coenzyme A binding" evidence="1">
        <location>
            <begin position="16"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="region of interest" description="Coenzyme A binding" evidence="1">
        <location>
            <begin position="73"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="region of interest" description="Coenzyme A binding" evidence="1">
        <location>
            <begin position="97"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="region of interest" description="Coenzyme A binding" evidence="1">
        <location>
            <begin position="137"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="1">
        <location>
            <begin position="244"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="1">
        <location>
            <position position="169"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="105"/>
        </location>
        <ligand>
            <name>CoA</name>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00742"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF11226.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="410" mass="44652" checksum="AA5FCA88ACE06833" modified="2006-05-30" version="1">MNNKALEGVRILDMTHVQAGPSATQLMAWLGADVIKVELPGRGDITRSQLRDVPNADSLYFTMLNSNKRSLTLNMKTPEGKALLEDLVQRCDVLVENFGPGVLDRAGFDWDRLQSLNPRLIYASIKGFGPGPYADCKAYENVAQCMGGSASTTGNEDGVPTVTGAQIGDSGTGVHCVVGILAALLHREQSGRGQRVEVAMQDSVLNLCRVKLRDQQRLSAGPLKEYPNDDFQDYVPRAGNASGGGQPGAALRCSPGGANDFVYVIIQPQGWEPLMRLCGREDLITHPQFATPEARLKCLAECFSIVEKWTRTKSKFEVMAALNDVDVPCGPILSMKDLIEDRSLYERGYMVELDHPERGRYVQLGCPISLSASPVEVERSPLLGEHTEEILEWLGRTPSQIVALKAAGAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="105" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LFA2</accession>
    <name>Q1LFA2_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Transcriptional regulator, AraC family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_4308</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11174.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518799.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4308"/>
    <dbReference type="EnsemblBacteria" id="ABF11174">
        <property type="protein sequence ID" value="ABF11174"/>
        <property type="gene ID" value="Rmet_4308"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4308"/>
    <dbReference type="eggNOG" id="COG2207">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_049704_5_0_4"/>
    <dbReference type="OMA" id="AREFRIS"/>
    <dbReference type="OrthoDB" id="1785082at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018060">
        <property type="entry name" value="HTH_AraC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018062">
        <property type="entry name" value="HTH_AraC-typ_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020449">
        <property type="entry name" value="Tscrpt_reg_HTH_AraC-type"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12833">
        <property type="entry name" value="HTH_18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00032">
        <property type="entry name" value="HTHARAC"/>
    </dbReference>
    <dbReference type="SMART" id="SM00342">
        <property type="entry name" value="HTH_ARAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00041">
        <property type="entry name" value="HTH_ARAC_FAMILY_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01124">
        <property type="entry name" value="HTH_ARAC_FAMILY_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH araC/xylS-type" evidence="2">
        <location>
            <begin position="217"/>
            <end position="318"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS01124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF11174.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="321" mass="35468" checksum="B6D2CE2A6F994665" modified="2006-05-30" version="1">MGNELGQLQRWSTDLVPATQRREYFQAATESAIIPFQFETPYSPDLSAKIEALALGPITVMHVAGSPHIARSTAAEISRQRARCLNLVLSRNVPWNLGYQSRVSMRPMDSFITDSQQALSLDIAFDYDFVNIGFDPQWLSEWVPQTGTVVGRQFGADSRWAVALNTFIAALQPTAILNAPLPPRLIVDQLGALILLASSGPDQVATHKHETSKPLNERILDCIRSRCTESTLVAPQVAADLGISVRSLHRVLAAQNETFGFLLMKARIDIAVRQLSSPAFNRLTVAAIGRRCGFTDDSHFTRVFRRHVGVTPLALRRARLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LFK8</accession>
    <name>Q1LFK8_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Aminotransferase, PLP-dependent</fullName>
            <ecNumber evidence="2">2.6.1.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_4201</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EC" id="2.6.1.-" evidence="2"/>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11068.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518691.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4201"/>
    <dbReference type="EnsemblBacteria" id="ABF11068">
        <property type="protein sequence ID" value="ABF11068"/>
        <property type="gene ID" value="Rmet_4201"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4201"/>
    <dbReference type="eggNOG" id="COG0436">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017584_4_5_4"/>
    <dbReference type="OMA" id="YPHMPTG"/>
    <dbReference type="OrthoDB" id="417859at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008483">
        <property type="term" value="F:transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009058">
        <property type="term" value="P:biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="SSF53383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aminotran_1_2" evidence="1">
        <location>
            <begin position="37"/>
            <end position="388"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF11068.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="405" mass="44666" checksum="CFCF8F507F19820B" modified="2006-05-30" version="1">MTADSGKRRFARIDRLPPYVFNITAELKMAARRRGEDIIDMSMGNPDGATPPHIVAKLVDAAQRPDTHGYSASKGIPRLRRAISHWYRERYDVDIDPDKEAIVTIGSKEGLAHLMLATLDRGDTVLVPDPSYPIHIYGAVIAGADIRSVPLTPGIDFFAELERAIRGSYPKPKMIVLGFPSNPTAACVELDFFERVIALARKHDIFVVHDLAYADIVFDGWKAPSIMQVPGAKDIAVEFFTLSKSYNMAGWRIGFMVGNPDLVAALTRIKSYHDYGTFTPLQVAAIAALEGDQQCVKEIAAQYQSRRDVLARGLIESGWPVDIPKASMYIWARIPEPYRALGSLEFAKQLLAKAKVSVSPGIGFGDYGDEYVRFALIENESRIRQAVRGIKAMFRADGLVTAPGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LG03</accession>
    <name>Q1LG03_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Periplasmic thioredoxin of cytochrome c-type biogenesis</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">ccmG</name>
        <name evidence="7" type="ordered locus">Rmet_4055</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="8">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="8">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the thioredoxin family. DsbE subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10923.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518550.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4055"/>
    <dbReference type="EnsemblBacteria" id="ABF10923">
        <property type="protein sequence ID" value="ABF10923"/>
        <property type="gene ID" value="Rmet_4055"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4055"/>
    <dbReference type="eggNOG" id="COG0526">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_042529_19_1_4"/>
    <dbReference type="OMA" id="MIGKPFP"/>
    <dbReference type="OrthoDB" id="1617952at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030288">
        <property type="term" value="C:outer membrane-bounded periplasmic space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015036">
        <property type="term" value="F:disulfide oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017004">
        <property type="term" value="P:cytochrome complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03010">
        <property type="entry name" value="TlpA_like_DsbE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004799">
        <property type="entry name" value="Periplasmic_diS_OxRdtase_DsbE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013740">
        <property type="entry name" value="Redoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017937">
        <property type="entry name" value="Thioredoxin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08534">
        <property type="entry name" value="Redoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="SSF52833"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00385">
        <property type="entry name" value="dsbE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00194">
        <property type="entry name" value="THIOREDOXIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0201">Cytochrome c-type biogenesis</keyword>
    <keyword evidence="4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0614">Plasmid</keyword>
    <keyword evidence="5" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Thioredoxin" evidence="6">
        <location>
            <begin position="32"/>
            <end position="178"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007758"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABF10923.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="183" mass="19791" checksum="CF06D35F7F536D5A" modified="2006-05-30" version="1">MMRFLLPLGVFLAIVIALAAGLRHDPRELPSSLVDKPAPAFVLPVLETSANANAQKFDPASLRGKVWVLNVWASWCTACRAEHPVLLGMAGQTQVPLYGLNYKDTRGAALEWLQRMGDPYKASAVDADGRVGIDLGVYGVPETFIIDAAGVVRYRQVGPVTQQVLEQKLLPLLKSLENGGSRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LG63</accession>
    <name>Q1LG63_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Beta-D-glucoside glucohydrolase, periplasmic</fullName>
            <ecNumber evidence="7">3.2.1.21</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">bglX</name>
        <name evidence="7" type="ordered locus">Rmet_3995</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="8">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="8">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the glycosyl hydrolase 3 family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.21" evidence="7"/>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10863.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3995"/>
    <dbReference type="CAZy" id="GH3">
        <property type="family name" value="Glycoside Hydrolase Family 3"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF10863">
        <property type="protein sequence ID" value="ABF10863"/>
        <property type="gene ID" value="Rmet_3995"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3995"/>
    <dbReference type="eggNOG" id="COG1472">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004542_5_1_4"/>
    <dbReference type="OMA" id="TLITDWD"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008422">
        <property type="term" value="F:beta-glucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102483">
        <property type="term" value="F:scopolin beta-glucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1700">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026891">
        <property type="entry name" value="Fn3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019800">
        <property type="entry name" value="Glyco_hydro_3_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002772">
        <property type="entry name" value="Glyco_hydro_3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036881">
        <property type="entry name" value="Glyco_hydro_3_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001764">
        <property type="entry name" value="Glyco_hydro_3_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036962">
        <property type="entry name" value="Glyco_hydro_3_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="Glycoside_hydrolase_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14310">
        <property type="entry name" value="Fn3-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00933">
        <property type="entry name" value="Glyco_hydro_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01915">
        <property type="entry name" value="Glyco_hydro_3_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00133">
        <property type="entry name" value="GLHYDRLASE3"/>
    </dbReference>
    <dbReference type="SMART" id="SM01217">
        <property type="entry name" value="Fn3_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="SSF51445"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52279">
        <property type="entry name" value="SSF52279"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00775">
        <property type="entry name" value="GLYCOSYL_HYDROL_F3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4 7" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="2 4 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0614">Plasmid</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193507" evidence="5">
        <location>
            <begin position="30"/>
            <end position="774"/>
        </location>
    </feature>
    <feature type="domain" description="Fn3_like" evidence="6">
        <location>
            <begin position="694"/>
            <end position="763"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU361161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM01217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABF10863.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="774" mass="83517" checksum="01BEB5D3B08CAC27" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="96" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LGG3</accession>
    <name>Q1LGG3_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Putative transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Rmet_3895</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="5">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="5">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10763.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518410.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3895"/>
    <dbReference type="EnsemblBacteria" id="ABF10763">
        <property type="protein sequence ID" value="ABF10763"/>
        <property type="gene ID" value="Rmet_3895"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3895"/>
    <dbReference type="eggNOG" id="COG2188">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_063236_4_2_4"/>
    <dbReference type="OMA" id="YYARIEF"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006744">
        <property type="term" value="P:ubiquinone biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd07377">
        <property type="entry name" value="WHTH_GntR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1410.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028978">
        <property type="entry name" value="Chorismate_lyase_/UTRA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000524">
        <property type="entry name" value="Tscrpt_reg_HTH_GntR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011663">
        <property type="entry name" value="UTRA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00392">
        <property type="entry name" value="GntR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07702">
        <property type="entry name" value="UTRA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00035">
        <property type="entry name" value="HTHGNTR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00345">
        <property type="entry name" value="HTH_GNTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00866">
        <property type="entry name" value="UTRA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64288">
        <property type="entry name" value="SSF64288"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50949">
        <property type="entry name" value="HTH_GNTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0456">Lyase</keyword>
    <keyword evidence="4" id="KW-0614">Plasmid</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0831">Ubiquinone biosynthesis</keyword>
    <feature type="domain" description="HTH gntR-type" evidence="3">
        <location>
            <begin position="13"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF10763.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="249" mass="27503" checksum="946A3C5BB6DF43C5" modified="2006-05-30" version="1">MPDANDLLSRSREPVYLQLATIFRRQIESGAWRPGEQIPSLEALCRQYGVARMTMHNALSRLDEEGLVERSRGRGTFVKAGGRECRRMALPINWNQAVAVGDKLSTEAIVESTADVPLPDDLGMPCDGRRADAYHFLHRAHRTDGRPFAVGEVYIAADVFARDPEAFRHGASVPVLDRFPGLKVNAARQRLSIVPAGAESASALELAVGAPVAELRRFACVDDVIVYYARIEFPTEYVCLDFDLLAPHS</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LGK4</accession>
    <name>Q1LGK4_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Putative monooxygenase</fullName>
            <ecNumber evidence="4">1.14.13.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Rmet_3854</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="5">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="5">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the NtaA/SnaA/DszA monooxygenase family.</text>
    </comment>
    <dbReference type="EC" id="1.14.13.-" evidence="4"/>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10722.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3854"/>
    <dbReference type="EnsemblBacteria" id="ABF10722">
        <property type="protein sequence ID" value="ABF10722"/>
        <property type="gene ID" value="Rmet_3854"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3854"/>
    <dbReference type="eggNOG" id="COG2141">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022256_1_2_4"/>
    <dbReference type="OMA" id="RMLGNFD"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01095">
        <property type="entry name" value="Nitrilotriacetate_monoxgenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011251">
        <property type="entry name" value="Luciferase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036661">
        <property type="entry name" value="Luciferase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016215">
        <property type="entry name" value="NTA_MOA"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00296">
        <property type="entry name" value="Bac_luciferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000337">
        <property type="entry name" value="NTA_MOA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51679">
        <property type="entry name" value="SSF51679"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03860">
        <property type="entry name" value="FMN_nitrolo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="2" id="KW-0288">FMN</keyword>
    <keyword evidence="4" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-0614">Plasmid</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Bac_luciferase" evidence="3">
        <location>
            <begin position="67"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="107"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="144"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="194"/>
            <end position="198"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="266"/>
            <end position="269"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00033748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR000337-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF10722.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="492" mass="53547" checksum="95F99730F05B7AE6" modified="2006-05-30" version="1">MFRAGGITILAFVRSFLPHASLCCHAKVSTHRAGARRRNARQQTATMTTPEAPRQLHLNLNALHAGFVPSAWRLPESDPRAFVDVQHYIRLAQVAEAGKFDAIFLADNASIADQINFRPITALEPTVLLACVAAATTHIGLVATASTSYNEPYNIARRFATLDHVSGGRAGWNVVTTADAGSARNFGRAAPPDHAQRYGRADEFTNVVKALWDSWEDDAFVGDRATGRFVDTDKLQPIAHHGQYFNVHGPLNLPRTPQGYPVLFQAGGSSDGRELAARHAEAVFSASQSFEESLAYRRDINQRAEALGRGPGAVKILPGLTTIIGATEAQARRRRDDLIDLIPWDYSLNRLAGTLGIAVDRLSLNAPLPNDLQLPANGNHTFFRATLALAQREGLTVRALIRELAGGGGHRVIVGTPEQIADDIEHWFRHGAADGFNLMPDVLPGGVEDFVDGVVPILQRRGLFRTEYTGRTLRDHMGLARPAGRTREQAAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LGL7</accession>
    <name>Q1LGL7_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Tannase and feruloyl esterase (Alpha/beta hydrolase family)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">tanA</name>
        <name evidence="7" type="ordered locus">Rmet_3841</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="8">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="8">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the tannase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10709.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518355.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3841"/>
    <dbReference type="ESTHER" id="ralme-q1lgl7">
        <property type="family name" value="Tannase"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF10709">
        <property type="protein sequence ID" value="ABF10709"/>
        <property type="gene ID" value="Rmet_3841"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3841"/>
    <dbReference type="eggNOG" id="COG1506">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014819_4_1_4"/>
    <dbReference type="OMA" id="AATDMGH"/>
    <dbReference type="OrthoDB" id="865015at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052689">
        <property type="term" value="F:carboxylic ester hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011118">
        <property type="entry name" value="Tannase/feruloyl_esterase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33938">
        <property type="entry name" value="PTHR33938"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07519">
        <property type="entry name" value="Tannase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="SSF53474"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0106">Calcium</keyword>
    <keyword evidence="4 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0614">Plasmid</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0719">Serine esterase</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="35"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABF10709.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="604" mass="62399" checksum="127D828846E3A5DF" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="95" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LGP1</accession>
    <name>Q1LGP1_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Rmet_3815</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="9">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="9">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Cell wall biogenesis; peptidoglycan biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the YkuD family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10685.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3815"/>
    <dbReference type="EnsemblBacteria" id="ABF10685">
        <property type="protein sequence ID" value="ABF10685"/>
        <property type="gene ID" value="Rmet_3815"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3815"/>
    <dbReference type="eggNOG" id="COG2989">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020360_3_4_4"/>
    <dbReference type="OMA" id="NIYLHHT"/>
    <dbReference type="OrthoDB" id="975147at2"/>
    <dbReference type="UniPathway" id="UPA00219"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071972">
        <property type="term" value="F:peptidoglycan L,D-transpeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009252">
        <property type="term" value="P:peptidoglycan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd16913">
        <property type="entry name" value="YkuD_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.101.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.440.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005490">
        <property type="entry name" value="LD_TPept_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045380">
        <property type="entry name" value="LD_TPept_scaffold_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002477">
        <property type="entry name" value="Peptidoglycan-bd-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036365">
        <property type="entry name" value="PGBD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036366">
        <property type="entry name" value="PGBDSf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038063">
        <property type="entry name" value="Transpep_catalytic_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01471">
        <property type="entry name" value="PG_binding_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20142">
        <property type="entry name" value="Scaffold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03734">
        <property type="entry name" value="YkuD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF141523">
        <property type="entry name" value="SSF141523"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47090">
        <property type="entry name" value="SSF47090"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0614">Plasmid</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Scaffold" evidence="7">
        <location>
            <begin position="43"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="domain" description="PG_binding_1" evidence="5">
        <location>
            <begin position="209"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="domain" description="YkuD" evidence="6">
        <location>
            <begin position="289"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004752"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF03734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF20142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ABF10685.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="540" mass="59721" checksum="F9D768EE1B7A1706" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="99" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LGY4</accession>
    <name>Q1LGY4_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Sulfate transporter (Permease)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Rmet_3720</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="8">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="8">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 5">Membrane</location>
            <topology evidence="1 5">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the SLC26A/SulP transporter (TC 2.A.53) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10592.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518239.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3720"/>
    <dbReference type="EnsemblBacteria" id="ABF10592">
        <property type="protein sequence ID" value="ABF10592"/>
        <property type="gene ID" value="Rmet_3720"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3720"/>
    <dbReference type="eggNOG" id="COG0659">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003182_13_2_4"/>
    <dbReference type="OMA" id="EAPHDIK"/>
    <dbReference type="OrthoDB" id="751256at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008271">
        <property type="term" value="F:secondary active sulfate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.750.24">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011547">
        <property type="entry name" value="SLC26A/SulP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001902">
        <property type="entry name" value="SLC26A/SulP_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002645">
        <property type="entry name" value="STAS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036513">
        <property type="entry name" value="STAS_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11814">
        <property type="entry name" value="PTHR11814"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01740">
        <property type="entry name" value="STAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00916">
        <property type="entry name" value="Sulfate_transp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52091">
        <property type="entry name" value="SSF52091"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00815">
        <property type="entry name" value="sulP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50801">
        <property type="entry name" value="STAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0614">Plasmid</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="42"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="66"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="91"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="117"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="154"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="196"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="222"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="260"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="344"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="370"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="401"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="domain" description="STAS" evidence="6">
        <location>
            <begin position="456"/>
            <end position="571"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU362052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABF10592.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="586" mass="63008" checksum="7E861884AD125B88" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="69" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LH51</accession>
    <name>Q1LH51_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Pilus assembly protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">cpaB</name>
        <name evidence="2" type="ordered locus">Rmet_3653</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10525.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518180.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3653"/>
    <dbReference type="EnsemblBacteria" id="ABF10525">
        <property type="protein sequence ID" value="ABF10525"/>
        <property type="gene ID" value="Rmet_3653"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3653"/>
    <dbReference type="eggNOG" id="COG3745">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_057068_0_1_4"/>
    <dbReference type="OMA" id="MNSKVLM"/>
    <dbReference type="OrthoDB" id="2060643at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017592">
        <property type="entry name" value="Pilus_assmbl_Flp-typ_CpaB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031571">
        <property type="entry name" value="RcpC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013974">
        <property type="entry name" value="SAF"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16976">
        <property type="entry name" value="RcpC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00858">
        <property type="entry name" value="SAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03177">
        <property type="entry name" value="pilus_cpaB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SAF" evidence="1">
        <location>
            <begin position="44"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00858"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF10525.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="319" mass="33011" checksum="B55A31D77289FC26" modified="2006-05-30" version="1">MSSKQIRIVAIVLLVLAGLLALLALQAARHTAAPAPAQGVVATHAVVVTTRAVPAGKPLPADALQVLQLPIDPGGAYQDVARVAGQVPLVNLGANVPVLESQLLAGLARQIPDGARAMAVAVDEVIGVGNQVQPGDFVDVFVVLRRDSQEIPQSQTRMLLSRLRVLAYGTGAVNEAPKQQGEQMMARREGAKTAVLSVPLEQVGTLVMAQQAGRLMLALRNPTDEAMPSDGMFADPPGVLQARAGVPPEAQRAPVDKAVAGVSLAGLVGEPRAPQTPAPAMRPAALPTGLPGMVATARRDPQNEGNGVEMIRAGKRAIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="64" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LH93</accession>
    <name>Q1LH93_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">CHAD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_3611</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10483.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518025.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3611"/>
    <dbReference type="EnsemblBacteria" id="ABF10483">
        <property type="protein sequence ID" value="ABF10483"/>
        <property type="gene ID" value="Rmet_3611"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3611"/>
    <dbReference type="eggNOG" id="COG5607">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1018919_0_0_4"/>
    <dbReference type="OMA" id="GVHQMRI"/>
    <dbReference type="OrthoDB" id="1878295at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.40.20.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007899">
        <property type="entry name" value="CHAD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038186">
        <property type="entry name" value="CHAD_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05235">
        <property type="entry name" value="CHAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00880">
        <property type="entry name" value="CHAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51708">
        <property type="entry name" value="CHAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CHAD" evidence="1">
        <location>
            <begin position="10"/>
            <end position="253"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF10483.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="253" mass="28395" checksum="0CF3AD0EF02FF386" modified="2006-05-30" version="1">MSRPPELHRKMRPAAAFVMLGGSGLRIVEAQLDALLQRDIAEDVHALRVAVRHLRAVTWAFGPILPRAVMERWKLALHDVADAASAVRDWDVFLSETLLPALAMQPGDPVLVALIDVAHARRAEARTLMVTQLTRYRQAPLPTLYRDLLHLSDPKTRHGQHGRMAAFARGRIRKARGRVRDLARVARDGQIAHIHALRIGNKRLRYAIEALSDVLPKRYRKRLHGKLIARQTELGGVIDDAVAHRLICECLAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LHH8</accession>
    <name>Q1LHH8_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Porin, OmpW family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">ompW1</name>
        <name evidence="3" type="ordered locus">Rmet_3526</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10398.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008640969.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3526"/>
    <dbReference type="EnsemblBacteria" id="ABF10398">
        <property type="protein sequence ID" value="ABF10398"/>
        <property type="gene ID" value="Rmet_3526"/>
    </dbReference>
    <dbReference type="GeneID" id="60822631"/>
    <dbReference type="KEGG" id="rme:Rmet_3526"/>
    <dbReference type="eggNOG" id="COG3047">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_042505_5_1_4"/>
    <dbReference type="OMA" id="AKQWSPT"/>
    <dbReference type="OrthoDB" id="2018542at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009279">
        <property type="term" value="C:cell outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011250">
        <property type="entry name" value="OMP/PagP_b-brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005618">
        <property type="entry name" value="OMPW"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36920">
        <property type="entry name" value="PTHR36920"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03922">
        <property type="entry name" value="OmpW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56925">
        <property type="entry name" value="SSF56925"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0998">Cell outer membrane</keyword>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193468" evidence="2">
        <location>
            <begin position="24"/>
            <end position="241"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF10398.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="241" mass="26069" checksum="870C125791214087" modified="2006-05-30" version="1">MKTIYKKMLVAGAAMALAGVAQAQSAGSNMVSFGWMRIMPTGHSEFLTIDSIGGFPVGAQKPNTGATIDSADTLGLTFAHFFTDNISAEFVLGVPPKHNVSGDRGYEKYGKLGEVRQWSPAIVAKWHFFDAKTKFRPYVGLGVNYTWFTGESVTNQSFVQGEFGPGSHMTASAKSSWNPVFNVGASYAVTDNWFVGFSVSYVPVTTTANFTTTLANGAQVQSHTKIKIDPVVTYLNVGYRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="104" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LHN0</accession>
    <name>Q1LHN0_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Coproporphyrinogen-III oxidase</fullName>
            <ecNumber evidence="11">1.3.98.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">hemN</name>
        <name evidence="15" type="ordered locus">Rmet_3474</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 11">
            <text>coproporphyrinogen III + 2 S-adenosyl-L-methionine = 2 5'-deoxyadenosine + 2 CO2 + 2 L-methionine + protoporphyrinogen IX</text>
            <dbReference type="Rhea" id="RHEA:15425"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:17319"/>
            <dbReference type="ChEBI" id="CHEBI:57307"/>
            <dbReference type="ChEBI" id="CHEBI:57309"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="EC" id="1.3.98.3"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="11 13">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="11 13">Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3 11">Porphyrin-containing compound metabolism; protoporphyrin-IX biosynthesis; protoporphyrinogen-IX from coproporphyrinogen-III (AdoMet route): step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 11">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 11">Belongs to the anaerobic coproporphyrinogen-III oxidase family.</text>
    </comment>
    <dbReference type="EC" id="1.3.98.3" evidence="11"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10346.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517904.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3474"/>
    <dbReference type="EnsemblBacteria" id="ABF10346">
        <property type="protein sequence ID" value="ABF10346"/>
        <property type="gene ID" value="Rmet_3474"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3474"/>
    <dbReference type="eggNOG" id="COG0635">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027579_3_0_4"/>
    <dbReference type="OMA" id="HILNLMC"/>
    <dbReference type="OrthoDB" id="1130951at2"/>
    <dbReference type="UniPathway" id="UPA00251">
        <property type="reaction ID" value="UER00323"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051989">
        <property type="term" value="F:coproporphyrinogen dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004109">
        <property type="term" value="F:coproporphyrinogen oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006782">
        <property type="term" value="P:protoporphyrinogen IX biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004558">
        <property type="entry name" value="Coprogen_oxidase_HemN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034505">
        <property type="entry name" value="Coproporphyrinogen-III_oxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaB/NifB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13932">
        <property type="entry name" value="PTHR13932"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000167">
        <property type="entry name" value="HemN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00538">
        <property type="entry name" value="hemN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 13" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="11" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8 11 13" id="KW-0408">Iron</keyword>
    <keyword evidence="9 11 13" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="7 11 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10 11" id="KW-0627">Porphyrin biosynthesis</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 11 12" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <feature type="domain" description="Radical SAM core" evidence="14">
        <location>
            <begin position="73"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="82"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="88"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="92"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="94"/>
            <end position="96"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="95"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="139"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="140"/>
            <end position="141"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="172"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="199"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="211"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="236"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="270"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="356"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <label>1</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004785"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005493"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRNR" id="PIRNR000167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR000167-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR000167-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="ABF10346.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="500" mass="55947" checksum="8B0F731E5A5EB0AC" modified="2006-05-30" version="1">MSAPMSDTPDTAIPSGLASSSTAFDRTALADFRELAGRIDGNGPRYTSYPTADRFHGEVGDAAYRRALDACRANVPAPLSLYVHIPFCENICYYCGCNKIITKDHTRSARYVDYLAREMAMAAEQLGERRAVMQSHWGGGTPTFLDPVEMRHVMSALHKHFELLPEGEHAIEIDPRRVGDDRMELLGELGFNRISLGVQDFDPEVQQAIHRVQSYEETNGVVRAARRLGFRSISMDLIYGLPHQTTQRFSATIDRVLEMRPDRLSVYSYAHLPHVFKPQRRIDPAALPPAAEKLDILVSTIERLTAAGYVYIGMDHFALPNDDLAIAQREGRLQRNFQGYATHAGYDQLGFGVSAIGAVAGHYAQNARTTDEYYAALDKGQLPIVRGFETSEDDRLRKFIIGELMCNGTLDIPAVEASYSINFAQKFAAELVDLDKLALDHLLRRTRNRIEITPLGRLLVRRIAMVFDHYLREDALRPKPAAPAANDAKVIPLVRYSRVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LHR2</accession>
    <name>Q1LHR2_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Acetylornithine deacetylase/succinyl-diaminopimelate desuccinylase related protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_3442</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10314.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517874.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3442"/>
    <dbReference type="EnsemblBacteria" id="ABF10314">
        <property type="protein sequence ID" value="ABF10314"/>
        <property type="gene ID" value="Rmet_3442"/>
    </dbReference>
    <dbReference type="GeneID" id="60822547"/>
    <dbReference type="KEGG" id="rme:Rmet_3442"/>
    <dbReference type="eggNOG" id="COG0624">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021802_2_3_4"/>
    <dbReference type="OMA" id="GDCAPHA"/>
    <dbReference type="OrthoDB" id="906744at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001261">
        <property type="entry name" value="ArgE/DapE_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036264">
        <property type="entry name" value="Bact_exopeptidase_dim_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002933">
        <property type="entry name" value="Peptidase_M20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011650">
        <property type="entry name" value="Peptidase_M20_dimer"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07687">
        <property type="entry name" value="M20_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01546">
        <property type="entry name" value="Peptidase_M20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55031">
        <property type="entry name" value="SSF55031"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00758">
        <property type="entry name" value="ARGE_DAPE_CPG2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="M20_dimer" evidence="2">
        <location>
            <begin position="207"/>
            <end position="317"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF10314.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="422" mass="45286" checksum="781E12A15F13615A" modified="2006-05-30" version="1">MTARDNMNPVETTLTQYIDQHRPQQEAFLAELVKVPSDNPPGDCDAHGKRAKELLEGLGFKVEAHKVPEDKVRAAGMISATNLLVRKTFGNGGPTIAMNAHGDVVPPGLGWTKDPYGGEIADSEHGPVMFGRGVAVSKSDFATYAYAVLALQEAEKQGAKLNGTVELQFTYDEETGGDIGPKFLLDEGLTKPDYAISAGFSYGITSAHNGCLHVEVTVKGKQGHAAMPHTGVDAIEAATHILQAIYGLRAELATRKSKVLGIDTATLNVGLIKGGINTNVVPDLVTFRVDRRMIPEEIGFDAEGELRAVVEKAAKERPGIEVKVERIILAEPLSELPGVEKLIGALKQRAESVFGVEIPVQGVPLYTDARHYTSRGIPTVLYGAGPRTLMEARGHNSDENLRLNDLNRATKVVALALSDLMA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-01-15" modified="2022-05-25" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LI18</accession>
    <name>RL10_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">50S ribosomal protein L10</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rplJ</name>
        <name type="ordered locus">Rmet_3336</name>
    </gene>
    <organism>
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Forms part of the ribosomal stalk, playing a central role in the interaction of the ribosome with GTP-bound translation factors.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the ribosomal stalk of the 50S ribosomal subunit. The N-terminus interacts with L11 and the large rRNA to form the base of the stalk. The C-terminus forms an elongated spine to which L12 dimers bind in a sequential fashion forming a multimeric L10(L12)X complex.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL10 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10208.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008649194.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q1LI18"/>
    <dbReference type="SMR" id="Q1LI18"/>
    <dbReference type="STRING" id="266264.Rmet_3336"/>
    <dbReference type="EnsemblBacteria" id="ABF10208">
        <property type="protein sequence ID" value="ABF10208"/>
        <property type="gene ID" value="Rmet_3336"/>
    </dbReference>
    <dbReference type="GeneID" id="60826581"/>
    <dbReference type="KEGG" id="rme:Rmet_3336"/>
    <dbReference type="eggNOG" id="COG0244">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_092227_0_0_4"/>
    <dbReference type="OMA" id="VRDQKQA"/>
    <dbReference type="OrthoDB" id="1470978at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015934">
        <property type="term" value="C:large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070180">
        <property type="term" value="F:large ribosomal subunit rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd05797">
        <property type="entry name" value="Ribosomal_L10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1730">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00362">
        <property type="entry name" value="Ribosomal_L10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022973">
        <property type="entry name" value="Ribosomal_L10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043141">
        <property type="entry name" value="Ribosomal_L10-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002363">
        <property type="entry name" value="Ribosomal_L10_eubac_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001790">
        <property type="entry name" value="Ribosomal_L10P"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11560">
        <property type="entry name" value="PTHR11560"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00466">
        <property type="entry name" value="Ribosomal_L10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160369">
        <property type="entry name" value="SSF160369"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01109">
        <property type="entry name" value="RIBOSOMAL_L10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <feature type="chain" id="PRO_1000005569" description="50S ribosomal protein L10">
        <location>
            <begin position="1"/>
            <end position="173"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00362"/>
        </source>
    </evidence>
    <sequence length="173" mass="17930" checksum="AF590F7276F59278" modified="2006-05-30" version="1">MPLNIEDKKAVVAEVSAQVAKAQTIVVAEYRGITVGDLTKLRATARTQGVYLRVLKNTLARRAVEGTPFAGLAEQMTGPLIYGISEDAVSSAKVLNDFAKTNDKLVLRAGSYNGNVLDAGAVKALASIPSRDELIAQLLGVMQAPVSGFARLLAAVAAKKAEGAPAEAEAPAA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-10-03" modified="2022-08-03" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LI53</accession>
    <name>RL18_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">50S ribosomal protein L18</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rplR</name>
        <name type="ordered locus">Rmet_3301</name>
    </gene>
    <organism>
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">This is one of the proteins that binds and probably mediates the attachment of the 5S RNA into the large ribosomal subunit, where it forms part of the central protuberance.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the 50S ribosomal subunit; part of the 5S rRNA/L5/L18/L25 subcomplex. Contacts the 5S and 23S rRNAs.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL18 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10173.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q1LI53"/>
    <dbReference type="SMR" id="Q1LI53"/>
    <dbReference type="STRING" id="266264.Rmet_3301"/>
    <dbReference type="EnsemblBacteria" id="ABF10173">
        <property type="protein sequence ID" value="ABF10173"/>
        <property type="gene ID" value="Rmet_3301"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3301"/>
    <dbReference type="eggNOG" id="COG0256">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_098841_0_1_4"/>
    <dbReference type="OMA" id="NKQIYAQ"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01337_B">
        <property type="entry name" value="Ribosomal_L18_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005484">
        <property type="entry name" value="Ribosomal_L18"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004389">
        <property type="entry name" value="Ribosomal_L18_bac-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12899">
        <property type="entry name" value="PTHR12899"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00861">
        <property type="entry name" value="Ribosomal_L18p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00060">
        <property type="entry name" value="L18_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <feature type="chain" id="PRO_0000251352" description="50S ribosomal protein L18">
        <location>
            <begin position="1"/>
            <end position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01337"/>
        </source>
    </evidence>
    <sequence length="119" mass="12861" checksum="23F5745AB471C46E" modified="2006-05-30" version="1">MMNKKDARLRRARQTRAKIAELKVNRLTVFRTNSHIYAQVYSPCGTQVLASASTAEAEVRKELNGNGATVAAATVVGKRVAEKAKAAGVETVAFDRAGFRFHGRVKALADAAREAGLKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="69" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LI96</accession>
    <name>Q1LI96_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Toluene transporter subunit: membrane component of ABC superfamily</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">yrbE</name>
        <name evidence="8" type="ordered locus">Rmet_3258</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cell inner membrane</location>
            <topology evidence="7">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the MlaE permease family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10130.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517730.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3258"/>
    <dbReference type="EnsemblBacteria" id="ABF10130">
        <property type="protein sequence ID" value="ABF10130"/>
        <property type="gene ID" value="Rmet_3258"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3258"/>
    <dbReference type="eggNOG" id="COG0767">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045686_1_1_4"/>
    <dbReference type="OMA" id="NYLVFPK"/>
    <dbReference type="OrthoDB" id="1253502at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043190">
        <property type="term" value="C:ATP-binding cassette (ABC) transporter complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003453">
        <property type="entry name" value="ABC_MlaE_Proteobac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030802">
        <property type="entry name" value="Permease_MalE"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30188">
        <property type="entry name" value="PTHR30188"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02405">
        <property type="entry name" value="MlaE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00056">
        <property type="entry name" value="TIGR00056"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="7" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="15"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="49"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="86"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="147"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="198"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="238"/>
            <end position="257"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007556"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU362044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ABF10130.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="258" mass="27346" checksum="3F2ECDAB2C450C9F" modified="2006-05-30" version="1">MNGFFTSIGSTVRQFVGGLGHATRMFLTMLALSPALLRRFRLVTDQVFFVGNLSLVIIAVSGLFVGFVLGLQGYYTLNRFGSEQALGLLVALSLVRELGPVVAALLFAGRAGTSLTAEIGLMKAGEQLSAMEMMAVNPLQRVIAPRFWAGVIAMPVLATIFSALGVLGGYLVGVQLIGVDAGAFWSQMQNGVDVRADVLNGVIKSFIFGITVTFIALYQGFEAKPTPEGVSRATTRTVVLASLAVLGLDFLLTALMFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="58" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LIX4</accession>
    <name>Q1LIX4_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_3029</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09902.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_003056203.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3029"/>
    <dbReference type="EnsemblBacteria" id="ABF09902">
        <property type="protein sequence ID" value="ABF09902"/>
        <property type="gene ID" value="Rmet_3029"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3029"/>
    <dbReference type="eggNOG" id="COG5489">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_109334_1_0_4"/>
    <dbReference type="OMA" id="SNEGREY"/>
    <dbReference type="OrthoDB" id="1730357at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007948">
        <property type="entry name" value="DUF736"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05284">
        <property type="entry name" value="DUF736"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF09902.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="104" mass="11321" checksum="5741A99E61AA813A" modified="2006-05-30" version="1">MANIGTFTADKDGFTGTLRTLTLNVKVKLVPNDKGDNEKAPDFRLQAASHDIGAAWKKTSEAGREYISVTLDDPSFPATVYARLIEGENGTHDLIWSRSKPQAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="101" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LIY7</accession>
    <name>Q1LIY7_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Two component heavy metal response transcriptional DNA binding regulator, winged helix family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">hmzR</name>
        <name evidence="5" type="ordered locus">Rmet_3016</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09889.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517534.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3016"/>
    <dbReference type="EnsemblBacteria" id="ABF09889">
        <property type="protein sequence ID" value="ABF09889"/>
        <property type="gene ID" value="Rmet_3016"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3016"/>
    <dbReference type="eggNOG" id="COG0745">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_30_1_4"/>
    <dbReference type="OMA" id="GHVVECS"/>
    <dbReference type="OrthoDB" id="1020672at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00383">
        <property type="entry name" value="trans_reg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001867">
        <property type="entry name" value="OmpR/PhoB-type_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039420">
        <property type="entry name" value="WalR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48111">
        <property type="entry name" value="PTHR48111"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00486">
        <property type="entry name" value="Trans_reg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00862">
        <property type="entry name" value="Trans_reg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51755">
        <property type="entry name" value="OMPR_PHOB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Response regulatory" evidence="3">
        <location>
            <begin position="2"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="domain" description="OmpR/PhoB-type" evidence="4">
        <location>
            <begin position="124"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="OmpR/PhoB-type" evidence="2">
        <location>
            <begin position="124"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="1">
        <location>
            <position position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABF09889.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="221" mass="24616" checksum="86F5BBED5304373D" modified="2006-05-30" version="1">MRVLIVEDSQTLAEALSQSLQSEGYACDTAADGVSALKFLASYRYDAIILDLMLPRLDGFGVLRALGREAHAAPVLVLSARELLDDRVRALDAGADDYLTKPFELAELLARLRALVRRPPQRDVPVLRHGDLEVDPRSRIARFKCIDLGLTPKEYGLLDLLLRRRGATLSRSQIFEHLYDSRSDASDKVVEVIVSTLRTKLAQCGLDELIVTRRGFGYILP</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LJ34</accession>
    <name>Q1LJ34_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 15 16">Protoporphyrinogen IX oxidase</fullName>
            <shortName evidence="15">PPO</shortName>
            <ecNumber evidence="15 16">1.3.99.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ordered locus">Rmet_2969</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="15 16">Catalyzes the oxidation of protoporphyrinogen IX to protoporphyrin IX.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 15 16">
            <text>3 A + protoporphyrinogen IX = 3 AH2 + protoporphyrin IX</text>
            <dbReference type="Rhea" id="RHEA:62000"/>
            <dbReference type="ChEBI" id="CHEBI:13193"/>
            <dbReference type="ChEBI" id="CHEBI:17499"/>
            <dbReference type="ChEBI" id="CHEBI:57306"/>
            <dbReference type="ChEBI" id="CHEBI:57307"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="15 16">
            <name>heme b</name>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
        </cofactor>
        <text evidence="15 16">Binds 1 heme b (iron(II)-protoporphyrin IX) group per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="4 15 16">Porphyrin-containing compound metabolism; protoporphyrin-IX biosynthesis; protoporphyrin-IX from protoporphyrinogen-IX: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="15">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3 15">Cell membrane</location>
            <topology evidence="3 15">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5 15 16">Belongs to the HemJ family.</text>
    </comment>
    <dbReference type="EC" id="1.3.99.-" evidence="15 16"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09842.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517505.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2969"/>
    <dbReference type="EnsemblBacteria" id="ABF09842">
        <property type="protein sequence ID" value="ABF09842"/>
        <property type="gene ID" value="Rmet_2969"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2969"/>
    <dbReference type="eggNOG" id="COG1981">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_125006_0_1_4"/>
    <dbReference type="OMA" id="VRLFIYH"/>
    <dbReference type="OrthoDB" id="1433260at2"/>
    <dbReference type="UniPathway" id="UPA00251">
        <property type="reaction ID" value="UER00324"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005887">
        <property type="term" value="C:integral component of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070818">
        <property type="term" value="F:protoporphyrinogen oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006782">
        <property type="term" value="P:protoporphyrinogen IX biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02239">
        <property type="entry name" value="HemJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005265">
        <property type="entry name" value="HemJ-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40255">
        <property type="entry name" value="PTHR40255"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03653">
        <property type="entry name" value="UPF0093"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004638">
        <property type="entry name" value="UCP004638"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 15 16" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8 15 16" id="KW-0349">Heme</keyword>
    <keyword evidence="13 15 16" id="KW-0408">Iron</keyword>
    <keyword evidence="14 15 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="10 15 16" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12 15" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 15" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="6"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="48"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="85"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="115"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="15">
        <location>
            <position position="8"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="15">
        <location>
            <position position="84"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001093"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00006501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00017504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRNR" id="PIRNR004638"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="ABF09842.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="140" mass="15969" checksum="713115C6B4492F3E" modified="2006-05-30" version="1">MLWVKAFHILFVTSWFAGLFYLPRIFVNLAMETDAASVKRLLLMARKLFRFMTMLAVPAVVFGLWLFLGYGIGRGAGQGWMHAKLALVLVLIGYHHGCGVLLRKFERGANTRSHTFYRWFNELPVLVLLAVVILVVVKPF</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="108" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LJD4</accession>
    <name>Q1LJD4_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Peptidyl-prolyl cis-trans isomerase</fullName>
            <ecNumber evidence="4">5.2.1.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">fkpB</name>
        <name evidence="6" type="ordered locus">Rmet_2869</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 3 4">
            <text>[protein]-peptidylproline (omega=180) = [protein]-peptidylproline (omega=0)</text>
            <dbReference type="Rhea" id="RHEA:16237"/>
            <dbReference type="Rhea" id="RHEA-COMP:10747"/>
            <dbReference type="Rhea" id="RHEA-COMP:10748"/>
            <dbReference type="ChEBI" id="CHEBI:83833"/>
            <dbReference type="ChEBI" id="CHEBI:83834"/>
            <dbReference type="EC" id="5.2.1.8"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the FKBP-type PPIase family.</text>
    </comment>
    <dbReference type="EC" id="5.2.1.8" evidence="4"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09742.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2869"/>
    <dbReference type="EnsemblBacteria" id="ABF09742">
        <property type="protein sequence ID" value="ABF09742"/>
        <property type="gene ID" value="Rmet_2869"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2869"/>
    <dbReference type="eggNOG" id="COG1047">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_098197_3_1_4"/>
    <dbReference type="OMA" id="FGQTEDH"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003755">
        <property type="term" value="F:peptidyl-prolyl cis-trans isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.50.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046357">
        <property type="entry name" value="PPIase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001179">
        <property type="entry name" value="PPIase_FKBP_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00254">
        <property type="entry name" value="FKBP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50059">
        <property type="entry name" value="FKBP_PPIASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4 6" id="KW-0413">Isomerase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3 4" id="KW-0697">Rotamase</keyword>
    <feature type="domain" description="PPIase FKBP-type" evidence="5">
        <location>
            <begin position="33"/>
            <end position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003915"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABF09742.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="172" mass="18596" checksum="4AC9877DF76929E0" modified="2006-05-30" version="1">MAAVDRLTPMTLQSTAAQAERDAAPARKTVQADSFLTLHYSIALENGTEVVSTFEEKPATLLLGQGQLVPTLEQALLGMAEGERTLYRLAPEQAFGPRNPELLQWVSLATLRENSSFEEDYAPGDLVEFNAPGGGKYAGVLKEYGETAALFDFNHPLAGQAILFDVQLIGIL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-09-19" modified="2022-05-25" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LJU8</accession>
    <name>UPPP_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Undecaprenyl-diphosphatase</fullName>
            <ecNumber evidence="1">3.6.1.27</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Bacitracin resistance protein</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Undecaprenyl pyrophosphate phosphatase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">uppP</name>
        <name type="ordered locus">Rmet_2705</name>
    </gene>
    <organism>
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the dephosphorylation of undecaprenyl diphosphate (UPP). Confers resistance to bacitracin.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>di-trans,octa-cis-undecaprenyl diphosphate + H2O = di-trans,octa-cis-undecaprenyl phosphate + H(+) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:28094"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58405"/>
            <dbReference type="ChEBI" id="CHEBI:60392"/>
            <dbReference type="EC" id="3.6.1.27"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="miscellaneous">
        <text>Bacitracin is thought to be involved in the inhibition of peptidoglycan synthesis by sequestering undecaprenyl diphosphate, thereby reducing the pool of lipid carrier available.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the UppP family.</text>
    </comment>
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        <property type="protein sequence ID" value="ABF09578.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="EnsemblBacteria" id="ABF09578">
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        <property type="gene ID" value="Rmet_2705"/>
    </dbReference>
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    <dbReference type="eggNOG" id="COG1968">
        <property type="taxonomic scope" value="Bacteria"/>
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    <dbReference type="OrthoDB" id="1826154at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
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        <property type="term" value="C:integral component of membrane"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="UniProtKB-UniRule"/>
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        <property type="term" value="P:dephosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="P:peptidoglycan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008360">
        <property type="term" value="P:regulation of cell shape"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="term" value="P:response to antibiotic"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01006">
        <property type="entry name" value="Undec_diphosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003824">
        <property type="entry name" value="UppP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30622">
        <property type="entry name" value="PTHR30622"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02673">
        <property type="entry name" value="BacA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0046">Antibiotic resistance</keyword>
    <keyword id="KW-0997">Cell inner membrane</keyword>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0133">Cell shape</keyword>
    <keyword id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-0573">Peptidoglycan synthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000250251" description="Undecaprenyl-diphosphatase">
        <location>
            <begin position="1"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="3"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="43"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="85"/>
            <end position="105"/>
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    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="109"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="178"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="203"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="238"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="269"/>
            <end position="289"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01006"/>
        </source>
    </evidence>
    <sequence length="293" mass="31757" checksum="6C6A7F9F5981D22B" modified="2006-05-30" version="1">MDIALALKAVILGIVEGLTEFLPISSTGHLILAGQLLDFNDEKGKIFEIVIQFGAILAVCWEFRQRIGTVISGLASDVKSQRFAVNVIVATIPAIVLALVFGKWIKAHLFNPITVATAFIIGGVVILLAEWREARRGTVSAPQGNALLEAAKAGAPRIESVDDLNWRDALKVGLAQCFALVPGTSRSGATIIGGMLFGLSRQVATEFSFFLAIPVIFGATVYELYKARALLSADDLGIFAVGFVFAFLSAFLCVRWLLRFVATHDFKPFAWYRIAFGIIVLLTAWTGVISWHA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LJV1</accession>
    <name>Q1LJV1_CUPMC</name>
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        <submittedName>
            <fullName evidence="2">Aldose 1-epimerase</fullName>
            <ecNumber evidence="2">5.1.3.3</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">galM</name>
        <name evidence="2" type="ordered locus">Rmet_2702</name>
    </gene>
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        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
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        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EC" id="5.1.3.3" evidence="2"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09575.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517273.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2702"/>
    <dbReference type="EnsemblBacteria" id="ABF09575">
        <property type="protein sequence ID" value="ABF09575"/>
        <property type="gene ID" value="Rmet_2702"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2702"/>
    <dbReference type="eggNOG" id="COG2017">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_057834_0_0_4"/>
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    <dbReference type="OrthoDB" id="710313at2"/>
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        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004034">
        <property type="term" value="F:aldose 1-epimerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
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        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Gal_mutarotase_sf_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014718">
        <property type="entry name" value="GH-type_carb-bd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01263">
        <property type="entry name" value="Aldose_epim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF74650">
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0413">Isomerase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="178"/>
            <end position="206"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF09575.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="301" mass="33478" checksum="537F88B027B4823C" modified="2006-05-30" version="1">MPTTSTQRYQGQDLLRIGNDHSYLLLAPEHGGRLVRWVHHGQDILFWPDDADWSRVAKIRGGNPLLFPFIGRHFVDGEAGKWRDPDGTVHSLPQHGFARDLPFTVTAHSDSSISLTLTSSDATHAGYPYDFAFAVTYRLTTNGLEVTLTTRNLGSRPLPYYAGHHFYFALPHEQRAESRLSMPPSARVRQKEDGSLTGTEPGDSTYSVADPRLQDTFHLLQAAGSVCLAMPSRTIDIELVTEGESGTPWYAVTTWTEHNASDFYCVEPWLGLPNAIAHGQGLRWLNANTEAHATCRLDVKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LK62</accession>
    <name>Q1LK62_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Glutathione s-transferase protein</fullName>
            <ecNumber evidence="2">2.5.1.18</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">gstN</name>
        <name evidence="2" type="ordered locus">Rmet_2587</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.5.1.18" evidence="2"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09464.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517167.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2587"/>
    <dbReference type="EnsemblBacteria" id="ABF09464">
        <property type="protein sequence ID" value="ABF09464"/>
        <property type="gene ID" value="Rmet_2587"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2587"/>
    <dbReference type="eggNOG" id="COG0625">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011226_12_0_4"/>
    <dbReference type="OMA" id="FRAINPV"/>
    <dbReference type="OrthoDB" id="1819702at2"/>
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        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004364">
        <property type="term" value="F:glutathione transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006749">
        <property type="term" value="P:glutathione metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004045">
        <property type="entry name" value="Glutathione_S-Trfase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034343">
        <property type="entry name" value="YibF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42673:SF12">
        <property type="entry name" value="PTHR42673:SF12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13417">
        <property type="entry name" value="GST_N_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="SSF52833"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50404">
        <property type="entry name" value="GST_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="GST N-terminal" evidence="1">
        <location>
            <begin position="1"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF09464.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="202" mass="22317" checksum="C419BD173A218482" modified="2006-05-30" version="1">MRLIGMLDSPYVRRVAIALRLLDLPFTHESVSVFRTFDQFRAINPVVKAPSLVCDNGVVLMDSTLIIDYAETLAGRSLMPAGLADRQHALRTIGLALAACEKVVQNVYEHNLRPEAKQHEPWLERIDGQRAAAFELLEQEIAKMPVPADARALDQVGLTAAVVWTFTQAMLPGKVDPARHPVLAAFTSAVERFAPFQDLPHA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-10-07" version="52" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LKA5</accession>
    <name>Q1LKA5_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_2544</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09421.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF09421">
        <property type="protein sequence ID" value="ABF09421"/>
        <property type="gene ID" value="Rmet_2544"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2544"/>
    <dbReference type="HOGENOM" id="CLU_2194779_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193623" evidence="1">
        <location>
            <begin position="25"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF09421.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="108" mass="11691" checksum="41AA66FD2A9F509A" modified="2006-05-30" version="1">MSSVRANLCRLPLLVLMGAMPALAVPPASDPALADTSGCTALINIVQESLRGELEVPCAEPNREACEAKNGQIRAMLEIVEQRRQRKADECETLAQVNRLLRTLPPKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="101" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LKE7</accession>
    <name>Q1LKE7_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Gluconate 2-dehydrogenase cytochrome c subunit (GADH cytochrome c subunit) (GA 2-DH cytochrome c subunit)</fullName>
            <ecNumber evidence="14">1.1.99.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="ordered locus">Rmet_2502</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>heme c</name>
            <dbReference type="ChEBI" id="CHEBI:61717"/>
        </cofactor>
        <text evidence="8">Binds 3 heme c groups covalently per subunit.</text>
    </comment>
    <comment type="PTM">
        <text evidence="9">Binds 3 heme groups per subunit.</text>
    </comment>
    <dbReference type="EC" id="1.1.99.-" evidence="14"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09379.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2502"/>
    <dbReference type="EnsemblBacteria" id="ABF09379">
        <property type="protein sequence ID" value="ABF09379"/>
        <property type="gene ID" value="Rmet_2502"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2502"/>
    <dbReference type="eggNOG" id="COG2010">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028594_0_0_4"/>
    <dbReference type="OMA" id="GDYSFED"/>
    <dbReference type="OrthoDB" id="1297285at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016614">
        <property type="term" value="F:oxidoreductase activity, acting on CH-OH group of donors"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.760.10">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009056">
        <property type="entry name" value="Cyt_c-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036909">
        <property type="entry name" value="Cyt_c-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008168">
        <property type="entry name" value="Cyt_C_IC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014353">
        <property type="entry name" value="Membr-bd_ADH_cyt_c"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00034">
        <property type="entry name" value="Cytochrom_C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13442">
        <property type="entry name" value="Cytochrome_CBB3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000018">
        <property type="entry name" value="Mb_ADH_cyt_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00605">
        <property type="entry name" value="CYTCHROMECIC"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46626">
        <property type="entry name" value="SSF46626"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51007">
        <property type="entry name" value="CYTC"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="2 8 10" id="KW-0349">Heme</keyword>
    <keyword evidence="6 9 10" id="KW-0408">Iron</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 9 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 12" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193631" evidence="12">
        <location>
            <begin position="29"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="domain" description="Cytochrome c" evidence="13">
        <location>
            <begin position="47"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="domain" description="Cytochrome c" evidence="13">
        <location>
            <begin position="193"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="domain" description="Cytochrome c" evidence="13">
        <location>
            <begin position="329"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="425"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="binding site" description="covalent" evidence="8">
        <location>
            <position position="61"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" description="covalent" evidence="8">
        <location>
            <position position="64"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="9">
        <location>
            <position position="65"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
            <label>1</label>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <feature type="binding site" description="covalent" evidence="8">
        <location>
            <position position="208"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" description="covalent" evidence="8">
        <location>
            <position position="211"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="9">
        <location>
            <position position="212"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
            <label>2</label>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <feature type="binding site" description="covalent" evidence="8">
        <location>
            <position position="342"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
            <label>3</label>
        </ligand>
    </feature>
    <feature type="binding site" description="covalent" evidence="8">
        <location>
            <position position="345"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
            <label>3</label>
        </ligand>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="9">
        <location>
            <position position="346"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
            <label>3</label>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR000018-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR000018-51"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ABF09379.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="450" mass="48062" checksum="3C935FAD1D448586" modified="2006-05-30" version="1">MKASKKATMMVAASALLAASAWTIGAFADDRQGDSATAAPVSGADAQLVERGRYLATVADCAACHTAKNGKPFAGGLGIESPIGRIYSTNITPDKSSGIGKFSYDDFDRAVRHGIARDGSTLYPAMPYASYAKVKPSDMQALYAYFMHGVQPIERANKATDIPWPLSMRWPLRFWRMAFAPEVATDDPPVSNDPVARGRYLVEGLAHCGACHTPRGWGLQEKALSDDGKTYLSGGVIDNFLAKNLRGDQKDGLGTWTQDEIFAFLKSGRTKHSAAFGGMSEVVENSMQHVNDADLTAIAAYLKTLTSVNPSAAVLTANASATDKLRAGTDRSNGALTFVDNCAACHRTSGSGYESTFPRIALSSTVNSNDPTSLIHIVLMGGEMPWTKTGPTHYGMPGFAKRLTDRDIADVLTFVRTSWGNQAPPVSAEQVAKVRKSVGAASQPKRPENN</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-01-15" modified="2022-08-03" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LKN5</accession>
    <name>ACPS_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Holo-[acyl-carrier-protein] synthase</fullName>
            <shortName evidence="1">Holo-ACP synthase</shortName>
            <ecNumber evidence="1">2.7.8.7</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">4'-phosphopantetheinyl transferase AcpS</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">acpS</name>
        <name type="ordered locus">Rmet_2414</name>
    </gene>
    <organism>
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Transfers the 4'-phosphopantetheine moiety from coenzyme A to a Ser of acyl-carrier-protein.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>apo-[ACP] + CoA = adenosine 3',5'-bisphosphate + H(+) + holo-[ACP]</text>
            <dbReference type="Rhea" id="RHEA:12068"/>
            <dbReference type="Rhea" id="RHEA-COMP:9685"/>
            <dbReference type="Rhea" id="RHEA-COMP:9690"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:58343"/>
            <dbReference type="ChEBI" id="CHEBI:64479"/>
            <dbReference type="EC" id="2.7.8.7"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the P-Pant transferase superfamily. AcpS family.</text>
    </comment>
    <dbReference type="EC" id="2.7.8.7" evidence="1"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09291.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517009.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q1LKN5"/>
    <dbReference type="SMR" id="Q1LKN5"/>
    <dbReference type="STRING" id="266264.Rmet_2414"/>
    <dbReference type="EnsemblBacteria" id="ABF09291">
        <property type="protein sequence ID" value="ABF09291"/>
        <property type="gene ID" value="Rmet_2414"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2414"/>
    <dbReference type="eggNOG" id="COG0736">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_089696_3_1_4"/>
    <dbReference type="OMA" id="DERHYAV"/>
    <dbReference type="OrthoDB" id="1893660at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008897">
        <property type="term" value="F:holo-[acyl-carrier-protein] synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006633">
        <property type="term" value="P:fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.470.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00101">
        <property type="entry name" value="AcpS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008278">
        <property type="entry name" value="4-PPantetheinyl_Trfase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037143">
        <property type="entry name" value="4-PPantetheinyl_Trfase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002582">
        <property type="entry name" value="ACPS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004568">
        <property type="entry name" value="Ppantetheine-prot_Trfase_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01648">
        <property type="entry name" value="ACPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56214">
        <property type="entry name" value="SSF56214"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00516">
        <property type="entry name" value="acpS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00556">
        <property type="entry name" value="pantethn_trn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword id="KW-0276">Fatty acid metabolism</keyword>
    <keyword id="KW-0444">Lipid biosynthesis</keyword>
    <keyword id="KW-0443">Lipid metabolism</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_1000008474" description="Holo-[acyl-carrier-protein] synthase">
        <location>
            <begin position="1"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="8"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="62"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00101"/>
        </source>
    </evidence>
    <sequence length="143" mass="16007" checksum="339EE1C2B5C8B438" modified="2006-05-30" version="1">MIYGVGTDIIQIDRVRGVMERTRGRFVEKILGPREMKIYLARKARSEKRGLAFLCTRFAAKEAFSKAIGLGMRWPMTWRAMELLNQPSGEPAPHCTGELAEWVRERGLTVRVSVSDEHEYAVAFAIAERGGQLAAPQSEPASN</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-10-07" version="58" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LKV8</accession>
    <name>Q1LKV8_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">bacHORMA_2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_2341</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09218.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2341"/>
    <dbReference type="EnsemblBacteria" id="ABF09218">
        <property type="protein sequence ID" value="ABF09218"/>
        <property type="gene ID" value="Rmet_2341"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2341"/>
    <dbReference type="eggNOG" id="ENOG50307TE">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_110705_0_0_4"/>
    <dbReference type="OMA" id="FRWDIDI"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040649">
        <property type="entry name" value="Bact_HORMA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036570">
        <property type="entry name" value="HORMA_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18173">
        <property type="entry name" value="bacHORMA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56019">
        <property type="entry name" value="SSF56019"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="bacHORMA_2" evidence="1">
        <location>
            <begin position="52"/>
            <end position="217"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF18173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF09218.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="217" mass="24212" checksum="75C44AB7C9CE663C" modified="2006-05-30" version="1">MSEGAARLGAKRAQKAQFSHHCTGLEPRNCHAGAGRCWASAVARPRRFPQVMSTVATYSYTHSVTYVTDNILKSLKDIILLSGLDPEHFADRWESNTRAIKTWLGTGDLRKVILEIYNPATDKLVTRWDIDIVYGWSDGDGSFWTDTEQLKYAIKKAGLLPSQAKYKLMLDTKPGRPDVEGWSKGSYRSTDGMVKQSLGSTVEHSGLAGQAGYWRQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LL50</accession>
    <name>Q1LL50_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">C-di-GMP phosphodiesterase A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">pdeA</name>
        <name evidence="5" type="ordered locus">Rmet_2247</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09126.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2247"/>
    <dbReference type="EnsemblBacteria" id="ABF09126">
        <property type="protein sequence ID" value="ABF09126"/>
        <property type="gene ID" value="Rmet_2247"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2247"/>
    <dbReference type="eggNOG" id="COG5001">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_70_20_4"/>
    <dbReference type="OMA" id="WHDPVLG"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01948">
        <property type="entry name" value="EAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01949">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00130">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.450">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012226">
        <property type="entry name" value="Diguanyl_cyclase/Pdiesterase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001633">
        <property type="entry name" value="EAL_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035919">
        <property type="entry name" value="EAL_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003018">
        <property type="entry name" value="GAF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029016">
        <property type="entry name" value="GAF-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000160">
        <property type="entry name" value="GGDEF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029787">
        <property type="entry name" value="Nucleotide_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000014">
        <property type="entry name" value="PAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035965">
        <property type="entry name" value="PAS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013767">
        <property type="entry name" value="PAS_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00563">
        <property type="entry name" value="EAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13185">
        <property type="entry name" value="GAF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00990">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00989">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13426">
        <property type="entry name" value="PAS_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005925">
        <property type="entry name" value="Dos"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00052">
        <property type="entry name" value="EAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00065">
        <property type="entry name" value="GAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00267">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00091">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF141868">
        <property type="entry name" value="SSF141868"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55073">
        <property type="entry name" value="SSF55073"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55785">
        <property type="entry name" value="SSF55785"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00254">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00229">
        <property type="entry name" value="sensory_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50883">
        <property type="entry name" value="EAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50887">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50112">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PAS" evidence="2">
        <location>
            <begin position="21"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="domain" description="PAS" evidence="2">
        <location>
            <begin position="151"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="domain" description="GGDEF" evidence="4">
        <location>
            <begin position="464"/>
            <end position="604"/>
        </location>
    </feature>
    <feature type="domain" description="EAL" evidence="3">
        <location>
            <begin position="613"/>
            <end position="867"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABF09126.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="873" mass="94098" checksum="E8EBA3B7BEA65E12" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="67" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LLF1</accession>
    <name>Q1LLF1_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">ABC superfamily transporter subunit (Periplasmic-binding component)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">yejA</name>
        <name evidence="2" type="ordered locus">Rmet_2146</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09025.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2146"/>
    <dbReference type="EnsemblBacteria" id="ABF09025">
        <property type="protein sequence ID" value="ABF09025"/>
        <property type="gene ID" value="Rmet_2146"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2146"/>
    <dbReference type="eggNOG" id="COG4166">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023171_0_0_4"/>
    <dbReference type="OMA" id="VLPKHWW"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043190">
        <property type="term" value="C:ATP-binding cassette (ABC) transporter complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030288">
        <property type="term" value="C:outer membrane-bounded periplasmic space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030678">
        <property type="entry name" value="Peptide/Ni-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039424">
        <property type="entry name" value="SBP_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000914">
        <property type="entry name" value="SBP_5_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30290">
        <property type="entry name" value="PTHR30290"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00496">
        <property type="entry name" value="SBP_bac_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002741">
        <property type="entry name" value="MppA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SBP_bac_5" evidence="1">
        <location>
            <begin position="155"/>
            <end position="563"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF09025.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="661" mass="74478" checksum="F2171FB807C8DB2B" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="105" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LLF4</accession>
    <name>Q1LLF4_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Transporter subunits of ABC superfamily: ATP-binding component</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">yejF</name>
        <name evidence="10" type="ordered locus">Rmet_2143</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09022.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2143"/>
    <dbReference type="EnsemblBacteria" id="ABF09022">
        <property type="protein sequence ID" value="ABF09022"/>
        <property type="gene ID" value="Rmet_2143"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2143"/>
    <dbReference type="eggNOG" id="COG4172">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_86_2_4"/>
    <dbReference type="OMA" id="RVCVMQR"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015833">
        <property type="term" value="P:peptide transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013563">
        <property type="entry name" value="Oligopep_ABC_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08352">
        <property type="entry name" value="oligo_HPY"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 8 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-1278">Translocase</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ABC transporter" evidence="9">
        <location>
            <begin position="23"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="9">
        <location>
            <begin position="293"/>
            <end position="541"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="57"/>
            <end position="64"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="333"/>
            <end position="340"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ABF09022.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="549" mass="60529" checksum="C6A810E3C7677BF0" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="97" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LLG3</accession>
    <name>Q1LLG3_CUPMC</name>
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        <recommendedName>
            <fullName evidence="5">Thioredoxin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">trxA</name>
        <name evidence="8" type="ordered locus">Rmet_2134</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the thioredoxin family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09013.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008647939.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2134"/>
    <dbReference type="EnsemblBacteria" id="ABF09013">
        <property type="protein sequence ID" value="ABF09013"/>
        <property type="gene ID" value="Rmet_2134"/>
    </dbReference>
    <dbReference type="GeneID" id="60821284"/>
    <dbReference type="KEGG" id="rme:Rmet_2134"/>
    <dbReference type="eggNOG" id="COG3118">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_090389_10_3_4"/>
    <dbReference type="OMA" id="QVGVAPK"/>
    <dbReference type="OrthoDB" id="1630944at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015035">
        <property type="term" value="F:protein-disulfide reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005746">
        <property type="entry name" value="Thioredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017937">
        <property type="entry name" value="Thioredoxin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00085">
        <property type="entry name" value="Thioredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000077">
        <property type="entry name" value="Thioredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="SSF52833"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01068">
        <property type="entry name" value="thioredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00194">
        <property type="entry name" value="THIOREDOXIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4 6" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Thioredoxin" evidence="7">
        <location>
            <begin position="1"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="disulfide bond" description="Redox-active" evidence="6">
        <location>
            <begin position="33"/>
            <end position="36"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRNR" id="PIRNR000077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR000077-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ABF09013.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="108" mass="11859" checksum="A0F935CF827C13A8" modified="2006-05-30" version="1">MSEQIKYVSDASFDADVLKSDKPVLLDFWAEWCGPCKMIAPILDEVAKDYGDKVQVAKINVDENQQVPAKFGIRGIPTLILFKNGQVAAQKVGALSKSQLTAFLDSQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="101" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LLS2</accession>
    <name>Q1LLS2_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transcriptional regulator, MarR-family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_2025</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08904.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2025"/>
    <dbReference type="EnsemblBacteria" id="ABF08904">
        <property type="protein sequence ID" value="ABF08904"/>
        <property type="gene ID" value="Rmet_2025"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2025"/>
    <dbReference type="eggNOG" id="COG1846">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_083287_18_5_4"/>
    <dbReference type="OMA" id="GLTPFHW"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00090">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011991">
        <property type="entry name" value="ArsR-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000835">
        <property type="entry name" value="HTH_MarR-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023187">
        <property type="entry name" value="Tscrpt_reg_MarR-type_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01047">
        <property type="entry name" value="MarR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00598">
        <property type="entry name" value="HTHMARR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00347">
        <property type="entry name" value="HTH_MARR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01117">
        <property type="entry name" value="HTH_MARR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50995">
        <property type="entry name" value="HTH_MARR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH marR-type" evidence="1">
        <location>
            <begin position="43"/>
            <end position="175"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF08904.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="193" mass="21507" checksum="C5E6FC38BA870A16" modified="2006-05-30" version="1">MANFYCCRIISTCLYLPRQRLQRQEMTSNTPGDLFDPDHYEIGKSVGYLLARAKSALSQGVEQEVSSLDMTHAQASCLMMLAKGEATTVTDLARNLNTDAGSVTRLLSRMEKRGLIERTRRDDDRRVVDLSLTAEGEAMVEKLPVVFCNVMRRHFVGFSQEEIETLRDMLLRLIANNGGIGSAASCPLDRNTG</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LLY5</accession>
    <name>Q1LLY5_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Molybdopterin synthase catalytic subunit</fullName>
            <ecNumber evidence="4">2.8.1.12</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">MPT synthase subunit 2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="7">Molybdenum cofactor biosynthesis protein E</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">Molybdopterin-converting factor large subunit</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">Molybdopterin-converting factor subunit 2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">moaE</name>
        <name evidence="11" type="ordered locus">Rmet_1962</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>2 [molybdopterin-synthase sulfur-carrier protein]-C-terminal Gly-NH-CH2-C(O)SH + cyclic pyranopterin phosphate + H2O = 2 [molybdopterin-synthase sulfur-carrier protein]-C-terminal Gly-Gly + 4 H(+) + molybdopterin</text>
            <dbReference type="Rhea" id="RHEA:26333"/>
            <dbReference type="Rhea" id="RHEA-COMP:12160"/>
            <dbReference type="Rhea" id="RHEA-COMP:12202"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:58698"/>
            <dbReference type="ChEBI" id="CHEBI:59648"/>
            <dbReference type="ChEBI" id="CHEBI:90619"/>
            <dbReference type="ChEBI" id="CHEBI:90778"/>
            <dbReference type="EC" id="2.8.1.12"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Cofactor biosynthesis; molybdopterin biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Heterotetramer of 2 MoaD subunits and 2 MoaE subunits. Also stable as homodimer. The enzyme changes between these two forms during catalysis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the MoaE family.</text>
    </comment>
    <dbReference type="EC" id="2.8.1.12" evidence="4"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08841.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516686.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1962"/>
    <dbReference type="EnsemblBacteria" id="ABF08841">
        <property type="protein sequence ID" value="ABF08841"/>
        <property type="gene ID" value="Rmet_1962"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1962"/>
    <dbReference type="eggNOG" id="COG0314">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_089568_2_1_4"/>
    <dbReference type="OMA" id="GEICLFV"/>
    <dbReference type="OrthoDB" id="1877633at2"/>
    <dbReference type="UniPathway" id="UPA00344"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030366">
        <property type="term" value="F:molybdopterin synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006777">
        <property type="term" value="P:Mo-molybdopterin cofactor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00756">
        <property type="entry name" value="MoaE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1170.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036563">
        <property type="entry name" value="MoaE_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003448">
        <property type="entry name" value="Mopterin_biosynth_MoaE"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02391">
        <property type="entry name" value="MoaE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54690">
        <property type="entry name" value="SSF54690"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005046"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005426"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011950"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00013858"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00026066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00029745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00030407"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00030781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00032474"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ABF08841.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="163" mass="17819" checksum="62C13AD88BA5F894" modified="2006-05-30" version="1">MAVRVQHEDFDLGAEVAALRAGNPGVGAVASFIGTVRDVSEGTRVSTMELEHYPGMTEKALETIEAAARERWPLLGVTIVHRVGLLQPLDQIVLVAVSSAHRGAAFAACEFIMDYLKSEAPFWKKEDTPEGARWVDARVSDDAALRRWGIESFNADPANNKEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-10-07" version="62" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LM22</accession>
    <name>Q1LM22_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_1925</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08804.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1925"/>
    <dbReference type="EnsemblBacteria" id="ABF08804">
        <property type="protein sequence ID" value="ABF08804"/>
        <property type="gene ID" value="Rmet_1925"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1925"/>
    <dbReference type="eggNOG" id="COG3064">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1389225_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF08804.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="196" mass="21526" checksum="E04349C0A3667603" modified="2006-05-30" version="1">MKRRRDPLAEGGGDRIDVATSKVGQNPSHANNWETDVGAMQKIVALVGLACFSVSVFAKWEYSENEDKMRGTKVKYAALASDNLVPLDFPYKPGTRLNIMIRKKSGAKDEVILQVDKGQIPCGYSGCKVSAKFDEGQVQTYAGAGTDSGRSDVIFVEASAKFLKALKSSKKVIVEVQFFQSGRQQFEFDSSGLEWK</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="70" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LM99</accession>
    <name>Q1LM99_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Ribulose-5-phosphate 4-epimerase or aldolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_1848</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08727.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008650227.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1848"/>
    <dbReference type="EnsemblBacteria" id="ABF08727">
        <property type="protein sequence ID" value="ABF08727"/>
        <property type="gene ID" value="Rmet_1848"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1848"/>
    <dbReference type="eggNOG" id="COG0235">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006033_1_2_4"/>
    <dbReference type="OMA" id="AFYIHAR"/>
    <dbReference type="OrthoDB" id="599627at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.225.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001303">
        <property type="entry name" value="Aldolase_II/adducin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036409">
        <property type="entry name" value="Aldolase_II/adducin_N_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00596">
        <property type="entry name" value="Aldolase_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01007">
        <property type="entry name" value="Aldolase_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53639">
        <property type="entry name" value="SSF53639"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aldolase_II" evidence="1">
        <location>
            <begin position="43"/>
            <end position="223"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM01007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF08727.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="277" mass="30485" checksum="173ED28B5379E954" modified="2006-05-30" version="1">MNAPQAKDEIGAGLSEEVKARYRNLPRPPQFATPAEERLHRKQRLAAAFRLFSKFGFDEGVAGHITARDPEFGDTFWVNPFGVHFSQVKVSNLIRCDHHGNVVEGDYPVNAAAFAIHSRVHQTRSDAVAAAHSHSTFGRAWSTLGRKLDPLTQDVCAFYNDHALYDDFGGVVVELDEGQRIANALGDNKAAILQNHGLLTVGKTVDEAAWWFITMERSCQVQLLAEAAAARTGDVPKAISDAAARQAYSIVGTSQAGWFQFQPLYARIVKEQPDLLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LMK5</accession>
    <name>Q1LMK5_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative glutamine amidotransferase</fullName>
            <ecNumber evidence="2">2.-.-.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_1742</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.-.-.-" evidence="2"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08621.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516473.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1742"/>
    <dbReference type="EnsemblBacteria" id="ABF08621">
        <property type="protein sequence ID" value="ABF08621"/>
        <property type="gene ID" value="Rmet_1742"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1742"/>
    <dbReference type="eggNOG" id="COG0121">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_042555_0_0_4"/>
    <dbReference type="OMA" id="PFRHGRW"/>
    <dbReference type="OrthoDB" id="693464at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006541">
        <property type="term" value="P:glutamine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026869">
        <property type="entry name" value="EgtC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017932">
        <property type="entry name" value="GATase_2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13230">
        <property type="entry name" value="GATase_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="SSF56235"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51278">
        <property type="entry name" value="GATASE_TYPE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glutamine amidotransferase type-2" evidence="1">
        <location>
            <begin position="2"/>
            <end position="279"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF08621.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="279" mass="31764" checksum="3FDBD4C8E8AF9565" modified="2006-05-30" version="1">MCRWLAYSGNPIQLETVLFRAQHSLIDQSLHSRLGHTTTNGDGFGMGWYAKHSEEPFRYRCIHPAWNDINLREAARAIRAAKFVAHIRAATDTPAQETNCHPFRYGRWLFAHNGLIRQYPEVRRELMLSIAPELFRWIEGSTDSEVMFYLALTFGLERDPREALQRTVGLVEHVGRRHGVDFPVNMTICATDGDQIVAVRYSSEGDSRSLFHSNSFRHLRELYPENPGIAAVGDDAVLILSEPLVDLPGAWAEIPEATFMQARQGTVDYYPFIPTYSEP</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LMV4</accession>
    <name>Q1LMV4_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Outer membrane protein (Porin)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Rmet_1639</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08522.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516367.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1639"/>
    <dbReference type="EnsemblBacteria" id="ABF08522">
        <property type="protein sequence ID" value="ABF08522"/>
        <property type="gene ID" value="Rmet_1639"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1639"/>
    <dbReference type="eggNOG" id="COG3203">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038238_2_0_4"/>
    <dbReference type="OMA" id="AYYNDAQ"/>
    <dbReference type="OrthoDB" id="1064531at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046930">
        <property type="term" value="C:pore complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015288">
        <property type="term" value="F:porin activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006811">
        <property type="term" value="P:ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00342">
        <property type="entry name" value="gram_neg_porins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.160.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033900">
        <property type="entry name" value="Gram_neg_porin_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023614">
        <property type="entry name" value="Porin_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13609">
        <property type="entry name" value="Porin_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0406">Ion transport</keyword>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0626">Porin</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1134">Transmembrane beta strand</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193633" evidence="4">
        <location>
            <begin position="24"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="domain" description="Porin_4" evidence="5">
        <location>
            <begin position="10"/>
            <end position="323"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF13609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABF08522.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="367" mass="39096" checksum="B8C2B554ACF39D18" modified="2006-05-30" version="1">MKTRITCLAALAILPTLPATAHADGSITLYGVVDAGVEYLTHVPNTAGGSNSVVRLSAGNLSTSRWGLRGSEDLGGGLKAIFTLENGFDLDTGNANQAGRLFGRQAFVGLQNQYGSLTLGRHQTVLYDFSLTYDPMAIATRYSVLMHDKWMSGRADNSLKLTGKLGAVYYGLFYSTGYDSKAGGEIPGDYKAGRNWSAAAGYDAGPLSARVVYDEVRGSTVATDDNRERRASIGASYNFGRGRAFAGYRWYYGDFATSSLRTNLYWLGASYQVTPAVTVTGAAYYTDVRNSSGDPYSFVLSGTYAFSKRTDLYAIAAYAQNSNGSAMGLSGFGPSLNANATTLTAATEQVGAGQNQFGAIIGIRHRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="50" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LMV6</accession>
    <name>Q1LMV6_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_1637</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08520.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008650745.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF08520">
        <property type="protein sequence ID" value="ABF08520"/>
        <property type="gene ID" value="Rmet_1637"/>
    </dbReference>
    <dbReference type="GeneID" id="60822288"/>
    <dbReference type="KEGG" id="rme:Rmet_1637"/>
    <dbReference type="eggNOG" id="ENOG502ZPPY">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_170903_0_0_4"/>
    <dbReference type="OMA" id="MQLHIPP"/>
    <dbReference type="OrthoDB" id="2083559at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF08520.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="105" mass="12009" checksum="94C8056A6FD92EAA" modified="2006-05-30" version="1">MEHLIRVQNEYDRQVLVWLRGRIGDAALMSAARRVAADGKKPYLSTICRLLNIRAPSRRSFAEEARKTHRAVGERYLAQIRQILNQPAAQMKTAGHEVRPQATLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="99" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LMZ2</accession>
    <name>Q1LMZ2_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Regulatory protein, MarR family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_1601</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08484.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516331.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF08484">
        <property type="protein sequence ID" value="ABF08484"/>
        <property type="gene ID" value="Rmet_1601"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1601"/>
    <dbReference type="eggNOG" id="COG1846">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_083287_18_5_4"/>
    <dbReference type="OMA" id="MEPHGIT"/>
    <dbReference type="OrthoDB" id="1744037at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000835">
        <property type="entry name" value="HTH_MarR-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01047">
        <property type="entry name" value="MarR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00598">
        <property type="entry name" value="HTHMARR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00347">
        <property type="entry name" value="HTH_MARR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50995">
        <property type="entry name" value="HTH_MARR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH marR-type" evidence="1">
        <location>
            <begin position="12"/>
            <end position="144"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF08484.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="145" mass="16526" checksum="358DADF2C4753F13" modified="2006-05-30" version="1">MKHYTPENFHLTQSIGFHLNKARNSLLLEMDSALRPLDINGQQMGILLALTRGMAGTPFEISKLLGIDSGLMTRMLDRLEEKGLLERSRSIDDRRVVNLTLTSKGRLVSAEIPSIAPEVLNERLKEFTPEEFDEFRRLLQKFVMG</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LN21</accession>
    <name>Q1LN21_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">6-carboxy-5,6,7,8-tetrahydropterin synthase</fullName>
            <ecNumber evidence="5">4.1.2.50</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Queuosine biosynthesis protein QueD</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">ptpS</name>
        <name evidence="11" type="ordered locus">Rmet_1572</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>7,8-dihydroneopterin 3'-triphosphate + H2O = 6-carboxy-5,6,7,8-tetrahydropterin + acetaldehyde + 2 H(+) + triphosphate</text>
            <dbReference type="Rhea" id="RHEA:27966"/>
            <dbReference type="ChEBI" id="CHEBI:15343"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:18036"/>
            <dbReference type="ChEBI" id="CHEBI:58462"/>
            <dbReference type="ChEBI" id="CHEBI:61032"/>
            <dbReference type="EC" id="4.1.2.50"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Purine metabolism; 7-cyano-7-deazaguanine biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the PTPS family. QueD subfamily.</text>
    </comment>
    <dbReference type="EC" id="4.1.2.50" evidence="5"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08455.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008649701.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1572"/>
    <dbReference type="EnsemblBacteria" id="ABF08455">
        <property type="protein sequence ID" value="ABF08455"/>
        <property type="gene ID" value="Rmet_1572"/>
    </dbReference>
    <dbReference type="GeneID" id="60822228"/>
    <dbReference type="KEGG" id="rme:Rmet_1572"/>
    <dbReference type="eggNOG" id="COG0720">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_111016_1_1_4"/>
    <dbReference type="OMA" id="HATVFNK"/>
    <dbReference type="OrthoDB" id="1770189at2"/>
    <dbReference type="UniPathway" id="UPA00391"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070497">
        <property type="term" value="F:6-carboxy-5,6,7,8-tetrahydropterin synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.479.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007115">
        <property type="entry name" value="6-PTP_synth/QueD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038418">
        <property type="entry name" value="6-PTP_synth/QueD_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12589">
        <property type="entry name" value="PTHR12589"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01242">
        <property type="entry name" value="PTPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 11" id="KW-0456">Lyase</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001293"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00018141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00031449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ABF08455.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="158" mass="17796" checksum="5EAB5B1E9CD67B98" modified="2006-05-30" version="1">MSKQVSITRRLEFDAGHRIPSHGGQCRNIHGHRYRLDLTLSGEVLHQEGAPDDGMILDFGDIKTLANEHLVSKWDHAFLIYRGDTALLNFLQSMDNHKTVVIDSIPTVENLAQVAFDILAPVFKDCFDHHLQLTRLVLFETPNCWAEVSAPLSLPAQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="97" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LN56</accession>
    <name>Q1LN56_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Carbamoyltransferase HypF</fullName>
            <ecNumber evidence="8">6.2.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">hypF2</name>
        <name evidence="12" type="ordered locus">Rmet_1537</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Involved in the maturation of [NiFe] hydrogenases. Along with HypE, it catalyzes the synthesis of the CN ligands of the active site iron of [NiFe]-hydrogenases. HypF functions as a carbamoyl transferase using carbamoylphosphate as a substrate and transferring the carboxamido moiety in an ATP-dependent reaction to the thiolate of the C-terminal cysteine of HypE yielding a protein-S-carboxamide.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + C-terminal L-cysteinyl-[HypE protein] + carbamoyl phosphate + H2O = AMP + C-terminal S-carboxamide-L-cysteinyl-[HypE protein] + diphosphate + H(+) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:55636"/>
            <dbReference type="Rhea" id="RHEA-COMP:14247"/>
            <dbReference type="Rhea" id="RHEA-COMP:14392"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58228"/>
            <dbReference type="ChEBI" id="CHEBI:76913"/>
            <dbReference type="ChEBI" id="CHEBI:139126"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>an acyl phosphate + H2O = a carboxylate + H(+) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:14965"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29067"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:59918"/>
            <dbReference type="EC" id="3.6.1.7"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 8">Protein modification; [NiFe] hydrogenase maturation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 8">Belongs to the carbamoyltransferase HypF family.</text>
    </comment>
    <dbReference type="EC" id="6.2.-.-" evidence="8"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08420.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516277.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1537"/>
    <dbReference type="EnsemblBacteria" id="ABF08420">
        <property type="protein sequence ID" value="ABF08420"/>
        <property type="gene ID" value="Rmet_1537"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1537"/>
    <dbReference type="eggNOG" id="COG0068">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009164_0_0_4"/>
    <dbReference type="OMA" id="VQHHYAH"/>
    <dbReference type="OrthoDB" id="48295at2"/>
    <dbReference type="UniPathway" id="UPA00335"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003998">
        <property type="term" value="F:acylphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016743">
        <property type="term" value="F:carboxyl- or carbamoyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003725">
        <property type="term" value="F:double-stranded RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016874">
        <property type="term" value="F:ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046944">
        <property type="term" value="P:protein carbamoylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001792">
        <property type="entry name" value="Acylphosphatase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036046">
        <property type="entry name" value="Acylphosphatase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017968">
        <property type="entry name" value="Acylphosphatase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004421">
        <property type="entry name" value="Carbamoyltransferase_HypF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017945">
        <property type="entry name" value="DHBP_synth_RibB-like_a/b_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041440">
        <property type="entry name" value="HypF_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006070">
        <property type="entry name" value="YrdC-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011125">
        <property type="entry name" value="Znf_HypF"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00708">
        <property type="entry name" value="Acylphosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17788">
        <property type="entry name" value="HypF_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01300">
        <property type="entry name" value="Sua5_yciO_yrdC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07503">
        <property type="entry name" value="zf-HYPF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006256">
        <property type="entry name" value="CMPcnvr_hdrg_mat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54975">
        <property type="entry name" value="SSF54975"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55821">
        <property type="entry name" value="SSF55821"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00143">
        <property type="entry name" value="hypF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00150">
        <property type="entry name" value="ACYLPHOSPHATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51160">
        <property type="entry name" value="ACYLPHOSPHATASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51163">
        <property type="entry name" value="YRDC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0436">Ligase</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Acylphosphatase-like" evidence="10">
        <location>
            <begin position="14"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="domain" description="YrdC-like" evidence="11">
        <location>
            <begin position="212"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="active site" evidence="9">
        <location>
            <position position="29"/>
        </location>
    </feature>
    <feature type="active site" evidence="9">
        <location>
            <position position="47"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004711"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRNR" id="PIRNR006256"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ABF08420.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="785" mass="84885" checksum="9E15C93ECC1CE020" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-01-15" modified="2022-05-25" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LNF5</accession>
    <name>RRF_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Ribosome-recycling factor</fullName>
            <shortName evidence="1">RRF</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Ribosome-releasing factor</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">frr</name>
        <name type="ordered locus">Rmet_1438</name>
    </gene>
    <organism>
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Responsible for the release of ribosomes from messenger RNA at the termination of protein biosynthesis. May increase the efficiency of translation by recycling ribosomes from one round of translation to another.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the RRF family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08321.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516188.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q1LNF5"/>
    <dbReference type="SMR" id="Q1LNF5"/>
    <dbReference type="STRING" id="266264.Rmet_1438"/>
    <dbReference type="EnsemblBacteria" id="ABF08321">
        <property type="protein sequence ID" value="ABF08321"/>
        <property type="gene ID" value="Rmet_1438"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1438"/>
    <dbReference type="eggNOG" id="COG0233">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_073981_2_0_4"/>
    <dbReference type="OMA" id="FNPMNNG"/>
    <dbReference type="OrthoDB" id="1674344at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006415">
        <property type="term" value="P:translational termination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00520">
        <property type="entry name" value="RRF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.132.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00040">
        <property type="entry name" value="RRF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002661">
        <property type="entry name" value="Ribosome_recyc_fac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023584">
        <property type="entry name" value="Ribosome_recyc_fac_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036191">
        <property type="entry name" value="RRF_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20982">
        <property type="entry name" value="PTHR20982"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01765">
        <property type="entry name" value="RRF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55194">
        <property type="entry name" value="SSF55194"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00496">
        <property type="entry name" value="frr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_1000003242" description="Ribosome-recycling factor">
        <location>
            <begin position="1"/>
            <end position="186"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00040"/>
        </source>
    </evidence>
    <sequence length="186" mass="20815" checksum="DD28236EDC643AA8" modified="2006-05-30" version="1">MTVADTKKSVEQKMQKSIEAFKADLAKVRTGRAHTGLLDHVQVDYYGSMVPISQVAAVSLADARTISVQPWEKKMVQAVEKAIRDGDLGLNPATMGDVIRVPMPPLTEERRRELTKVVKGEAEGAKVAVRNLRRDANEQFKKLVKDKAISEDDERRGQDEVQKLTDKFVAEIDKLVAEKEKEIMTV</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-03-18" modified="2022-08-03" version="92" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LNF6</accession>
    <name>PYRH_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Uridylate kinase</fullName>
            <shortName evidence="1">UK</shortName>
            <ecNumber evidence="1">2.7.4.22</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Uridine monophosphate kinase</fullName>
            <shortName evidence="1">UMP kinase</shortName>
            <shortName evidence="1">UMPK</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">pyrH</name>
        <name type="ordered locus">Rmet_1437</name>
    </gene>
    <organism>
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the reversible phosphorylation of UMP to UDP.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + UMP = ADP + UDP</text>
            <dbReference type="Rhea" id="RHEA:24400"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57865"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.22"/>
        </reaction>
    </comment>
    <comment type="activity regulation">
        <text evidence="1">Inhibited by UTP.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Pyrimidine metabolism; CTP biosynthesis via de novo pathway; UDP from UMP (UMPK route): step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homohexamer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the UMP kinase family.</text>
    </comment>
    <comment type="sequence caution" evidence="2">
        <conflict type="erroneous initiation">
            <sequence resource="EMBL-CDS" id="ABF08320" version="1"/>
        </conflict>
    </comment>
    <dbReference type="EC" id="2.7.4.22" evidence="1"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08320.1"/>
        <property type="status" value="ALT_INIT"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008652269.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q1LNF6"/>
    <dbReference type="SMR" id="Q1LNF6"/>
    <dbReference type="STRING" id="266264.Rmet_1437"/>
    <dbReference type="EnsemblBacteria" id="ABF08320">
        <property type="protein sequence ID" value="ABF08320"/>
        <property type="gene ID" value="Rmet_1437"/>
    </dbReference>
    <dbReference type="GeneID" id="60822194"/>
    <dbReference type="KEGG" id="rme:Rmet_1437"/>
    <dbReference type="eggNOG" id="COG0528">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033861_0_0_4"/>
    <dbReference type="OrthoDB" id="1043239at2"/>
    <dbReference type="UniPathway" id="UPA00159">
        <property type="reaction ID" value="UER00275"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033862">
        <property type="term" value="F:UMP kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044210">
        <property type="term" value="P:'de novo' CTP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04254">
        <property type="entry name" value="AAK_UMPK-PyrH-Ec"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1160.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01220_B">
        <property type="entry name" value="PyrH_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036393">
        <property type="entry name" value="AceGlu_kinase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001048">
        <property type="entry name" value="Asp/Glu/Uridylate_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011817">
        <property type="entry name" value="Uridylate_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015963">
        <property type="entry name" value="Uridylate_kinase_bac"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00696">
        <property type="entry name" value="AA_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005650">
        <property type="entry name" value="Uridylate_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53633">
        <property type="entry name" value="SSF53633"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02075">
        <property type="entry name" value="pyrH_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0418">Kinase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0665">Pyrimidine biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000323932" description="Uridylate kinase">
        <location>
            <begin position="1"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="10"/>
            <end position="13"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="52"/>
        </location>
        <ligand>
            <name>UMP</name>
            <dbReference type="ChEBI" id="CHEBI:57865"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="53"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="57"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="72"/>
        </location>
        <ligand>
            <name>UMP</name>
            <dbReference type="ChEBI" id="CHEBI:57865"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="133"/>
            <end position="140"/>
        </location>
        <ligand>
            <name>UMP</name>
            <dbReference type="ChEBI" id="CHEBI:57865"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="160"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="166"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="169"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01220"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="236" mass="25187" checksum="AB94B03076244849" modified="2008-03-18" version="2">MPAYKRVLLKLSGEALMGDDAFGINRATIEGMVNDIAEIVKLGVQVAVVIGGGNIFRGVAGGAAGMDRATADYMGMLATMMNALALQDAMRHASIEGRVQSALRMDQVVEPYIRPRAIRQLEEGKVVIFAAGTGNPFFTTDTAAALRGSEIGAEIVLKATKVDGVYTADPKKDPSATRYTTISFDEAISRNLQVMDATAFALCRDQKLPIKVFSIVKPGALKRVILGEDEGTLVHV</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-01-15" modified="2022-08-03" version="97" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LNG5</accession>
    <name>DNLJ_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DNA ligase</fullName>
            <ecNumber evidence="1">6.5.1.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Polydeoxyribonucleotide synthase [NAD(+)]</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">ligA</name>
        <name type="ordered locus">Rmet_1428</name>
    </gene>
    <organism>
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of damaged DNA.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>NAD(+) + (deoxyribonucleotide)n-3'-hydroxyl + 5'-phospho-(deoxyribonucleotide)m = (deoxyribonucleotide)n+m + AMP + beta-nicotinamide D-nucleotide.</text>
            <dbReference type="EC" id="6.5.1.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the NAD-dependent DNA ligase family. LigA subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.5.1.2" evidence="1"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08311.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516179.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q1LNG5"/>
    <dbReference type="SMR" id="Q1LNG5"/>
    <dbReference type="STRING" id="266264.Rmet_1428"/>
    <dbReference type="PRIDE" id="Q1LNG5"/>
    <dbReference type="EnsemblBacteria" id="ABF08311">
        <property type="protein sequence ID" value="ABF08311"/>
        <property type="gene ID" value="Rmet_1428"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1428"/>
    <dbReference type="eggNOG" id="COG0272">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007764_2_1_4"/>
    <dbReference type="OMA" id="HDVEHEI"/>
    <dbReference type="OrthoDB" id="241401at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003911">
        <property type="term" value="F:DNA ligase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00114">
        <property type="entry name" value="LIGANc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10190">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01588">
        <property type="entry name" value="DNA_ligase_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001357">
        <property type="entry name" value="BRCT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036420">
        <property type="entry name" value="BRCT_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041663">
        <property type="entry name" value="DisA/LigA_HHH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018239">
        <property type="entry name" value="DNA_ligase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033136">
        <property type="entry name" value="DNA_ligase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001679">
        <property type="entry name" value="DNAligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013839">
        <property type="entry name" value="DNAligase_adenylation"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013840">
        <property type="entry name" value="DNAligase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003583">
        <property type="entry name" value="Hlx-hairpin-Hlx_DNA-bd_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004150">
        <property type="entry name" value="NAD_DNA_ligase_OB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010994">
        <property type="entry name" value="RuvA_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004149">
        <property type="entry name" value="Znf_DNAligase_C4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00533">
        <property type="entry name" value="BRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01653">
        <property type="entry name" value="DNA_ligase_aden"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03120">
        <property type="entry name" value="DNA_ligase_OB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03119">
        <property type="entry name" value="DNA_ligase_ZBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12826">
        <property type="entry name" value="HHH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001604">
        <property type="entry name" value="LigA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00292">
        <property type="entry name" value="BRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00278">
        <property type="entry name" value="HhH1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00532">
        <property type="entry name" value="LIGANc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47781">
        <property type="entry name" value="SSF47781"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52113">
        <property type="entry name" value="SSF52113"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00575">
        <property type="entry name" value="dnlj"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50172">
        <property type="entry name" value="BRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01055">
        <property type="entry name" value="DNA_LIGASE_N1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01056">
        <property type="entry name" value="DNA_LIGASE_N2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0227">DNA damage</keyword>
    <keyword id="KW-0234">DNA repair</keyword>
    <keyword id="KW-0235">DNA replication</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0464">Manganese</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0520">NAD</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <feature type="chain" id="PRO_0000313388" description="DNA ligase">
        <location>
            <begin position="1"/>
            <end position="721"/>
        </location>
    </feature>
    <feature type="domain" description="BRCT" evidence="1">
        <location>
            <begin position="639"/>
            <end position="721"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="active site" description="N6-AMP-lysine intermediate" evidence="1">
        <location>
            <position position="164"/>
        </location>
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    <feature type="binding site" evidence="1">
        <location>
            <begin position="48"/>
            <end position="52"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="97"/>
            <end position="98"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="162"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="185"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="221"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="338"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="362"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="456"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="459"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="474"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="480"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="721" mass="77306" checksum="ACDD91DB853D85B1" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LNH2</accession>
    <name>Q1LNH2_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative arsenate reductase or related protein (Glutaredoxin family)</fullName>
            <ecNumber evidence="3">1.20.4.1</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">arsC3</name>
        <name evidence="3" type="ordered locus">Rmet_1421</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the ArsC family.</text>
    </comment>
    <dbReference type="EC" id="1.20.4.1" evidence="3"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08304.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008649082.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1421"/>
    <dbReference type="EnsemblBacteria" id="ABF08304">
        <property type="protein sequence ID" value="ABF08304"/>
        <property type="gene ID" value="Rmet_1421"/>
    </dbReference>
    <dbReference type="GeneID" id="60822178"/>
    <dbReference type="KEGG" id="rme:Rmet_1421"/>
    <dbReference type="eggNOG" id="COG1393">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_116644_2_1_4"/>
    <dbReference type="OMA" id="FGIKNCD"/>
    <dbReference type="OrthoDB" id="1606021at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008794">
        <property type="term" value="F:arsenate reductase (glutaredoxin) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006660">
        <property type="entry name" value="Arsenate_reductase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006504">
        <property type="entry name" value="Tscrpt_reg_Spx/MgsR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30041">
        <property type="entry name" value="PTHR30041"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03960">
        <property type="entry name" value="ArsC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="SSF52833"/>
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    </dbReference>
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        <property type="entry name" value="arsC_related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51353">
        <property type="entry name" value="ARSC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF08304.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="115" mass="12782" checksum="05E7B1C69E3CECC9" modified="2006-05-30" version="1">MTTLLYGIPNCDTVKRARTWLESNGVDYTFHDFKKQGVDEKMLRAWLKHVPLTTLLNKKGTTYRALSDADKTRAESEAGAIALMQTSPSLIKRPVLDRDGKVSVGFSADGYAALF</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LNW1</accession>
    <name>Q1LNW1_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Transposase ISRme5 (Copy b, CMGI-2)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">tnpA</name>
        <name evidence="3" type="ordered locus">Rmet_1280</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
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        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08165.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF08165">
        <property type="protein sequence ID" value="ABF08165"/>
        <property type="gene ID" value="Rmet_1280"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1280"/>
    <dbReference type="eggNOG" id="COG2801">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027402_15_1_4"/>
    <dbReference type="OMA" id="CANDGRF"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001584">
        <property type="entry name" value="Integrase_cat-core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00665">
        <property type="entry name" value="rve"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="SSF53098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50994">
        <property type="entry name" value="INTEGRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Integrase catalytic" evidence="2">
        <location>
            <begin position="122"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="356"/>
            <end position="391"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF08165.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="463" mass="51793" checksum="FB8A835410D78E4E" modified="2006-05-30" version="1">MHLRLHKNATTTPRIRAEIQVSKEPMRVLAQRFGVSVSTIARWKKRASVHDASHTPHRLQTTLTPAQESIVLVLRKSLGLSLDDLLAVVREFIHPTVSRAALHRMLKRHGVSAREALSVDRPRTKPFKAYEPGFVHIDVKYLPQMADETTRRYLFVAIDRATRWVFVRVYASKSATNARRFLKELHKAAAFRIRTILTDNGKEFTDRFITRGERTPTGRHQFDQLCEELGIEHRLTRPKHPQTNGMVERFNGRIADILRTHHFHSGEELEATILRYVWLYNHQLPQKALGHVSPIQAMKQWQRSHPELFNRRVTNQPGHDTISALPLSVLRNTRLDITRLLVSCFHQTPPRARCPFEGHRGTGSAGPQVPSPCPAPKARQSGGKPRSGAGGCQQILGSCSPLSQSTTRRPPKRVAICTKWCGSVVTSPMRAASCPSGWARMAASTASAASGATTAISLPSLAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LPA4</accession>
    <name>Q1LPA4_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Major facilitator superfamily (MFS_1) transporter and phospholipid/glycerol acyltransferase</fullName>
            <ecNumber evidence="3">2.3.1.51</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_1136</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.3.1.51" evidence="3"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08022.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515923.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1136"/>
    <dbReference type="EnsemblBacteria" id="ABF08022">
        <property type="protein sequence ID" value="ABF08022"/>
        <property type="gene ID" value="Rmet_1136"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1136"/>
    <dbReference type="eggNOG" id="COG0204">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029603_0_0_4"/>
    <dbReference type="OMA" id="GNGMVEM"/>
    <dbReference type="OrthoDB" id="961884at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003841">
        <property type="term" value="F:1-acylglycerol-3-phosphate O-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002123">
        <property type="entry name" value="Plipid/glycerol_acylTrfase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01553">
        <property type="entry name" value="Acyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00563">
        <property type="entry name" value="PlsC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="SSF103473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="55"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="86"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="155"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="235"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="271"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="302"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="345"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="379"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="domain" description="PlsC" evidence="2">
        <location>
            <begin position="463"/>
            <end position="579"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF08022.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="634" mass="68955" checksum="155838C638CA51B7" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LPA9</accession>
    <name>Q1LPA9_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">UPF0761 membrane protein Rmet_1131</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">rbn</name>
        <name evidence="7" type="ordered locus">Rmet_1131</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cell inner membrane</location>
            <topology evidence="6">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the UPF0761 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08017.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515918.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1131"/>
    <dbReference type="EnsemblBacteria" id="ABF08017">
        <property type="protein sequence ID" value="ABF08017"/>
        <property type="gene ID" value="Rmet_1131"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1131"/>
    <dbReference type="eggNOG" id="COG1295">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032288_1_2_4"/>
    <dbReference type="OMA" id="MTMMTIE"/>
    <dbReference type="OrthoDB" id="1421045at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005887">
        <property type="term" value="C:integral component of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00672">
        <property type="entry name" value="UPF0761"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023679">
        <property type="entry name" value="UPF0761_bac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017039">
        <property type="entry name" value="Virul_fac_BrkB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30213">
        <property type="entry name" value="PTHR30213"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03631">
        <property type="entry name" value="Virul_fac_BrkB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00765">
        <property type="entry name" value="yihY_not_rbn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="2 6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="37"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="104"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="145"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="181"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="216"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="258"/>
            <end position="278"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00672"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABF08017.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="447" mass="49649" checksum="6982B12971600732" modified="2006-05-30" version="1">MTAVRIARLRREWNLQKVRALARYALRRAGEDRLPQVSASLTFTTVLAVVPVLTVAFALLAAFPVFRDFRGQIEMFLFQNLIPGGNISDSVSRYIGQFSRSARGLTALGLAGLMVTSVLTMLTVEDALNAIWRVRRRRPFAQRVLVFWAVLTLGPVLIGASLSVSSYLMSVSMGYVGNLPFGVGFVISLVPVLLSALAFAFLYMAVPNAYVEWRDAIVAGIVAAVAFEVAKRGFGYFITHIPTYTAVYGTFAVLPLFLLWMYVSWLVTLLGATIAANLPVIREGHWRRRTFAGSEFFDALGILYLLYRARDEVPRSVGELDMGRKLRMEADYLSGLLTKLKAMHLVGKLQQDRGQAHWALLCDPSQVTLRTLHDRLVLNMARLPRTALAQQMQGAEALRSILENPQLEQTLDAVFRQQTAGAAANEETGHRDRKVVEVVPPGWHGQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LPS2</accession>
    <name>Q1LPS2_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Acyl-CoA dehydrogenase (ACAD)</fullName>
            <ecNumber evidence="8">1.3.99.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Rmet_0968</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the acyl-CoA dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.3.99.-" evidence="8"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07854.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515772.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0968"/>
    <dbReference type="EnsemblBacteria" id="ABF07854">
        <property type="protein sequence ID" value="ABF07854"/>
        <property type="gene ID" value="Rmet_0968"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0968"/>
    <dbReference type="eggNOG" id="COG1960">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018204_1_2_4"/>
    <dbReference type="OMA" id="DTVGNRG"/>
    <dbReference type="OrthoDB" id="760677at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016627">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-CH group of donors"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.540.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.110.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006091">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046373">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036250">
        <property type="entry name" value="AcylCo_DH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009075">
        <property type="entry name" value="AcylCo_DH/oxidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013786">
        <property type="entry name" value="AcylCoA_DH/ox_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037069">
        <property type="entry name" value="AcylCoA_DH/ox_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009100">
        <property type="entry name" value="AcylCoA_DH/oxidase_NM_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00441">
        <property type="entry name" value="Acyl-CoA_dh_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02770">
        <property type="entry name" value="Acyl-CoA_dh_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02771">
        <property type="entry name" value="Acyl-CoA_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47203">
        <property type="entry name" value="SSF47203"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56645">
        <property type="entry name" value="SSF56645"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0274">FAD</keyword>
    <keyword evidence="3" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Acyl-CoA_dh_N" evidence="7">
        <location>
            <begin position="7"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA_dh_M" evidence="6">
        <location>
            <begin position="137"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA_dh_1" evidence="5">
        <location>
            <begin position="250"/>
            <end position="398"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF02770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF02771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ABF07854.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="420" mass="47062" checksum="1F10AB8F6994CBAD" modified="2006-05-30" version="1">MQFEYTPKVKEMQARLLAFFDQHIYPNEKRFYAEIQANRAAGNAWIPTKVIEELKPLAREAGLWNLFLPRSKRAPEGLSNLEYAPMCEIMGRVPWSAEVFNCAAPDTGNMETLERYASEELKDEWLEPLLRGEIRSAFLMTEPAVASSDATNIECRIERDGDHYVINGTKWWSSGAGDPRCKVYIVMGKTDPNAGRHEQQSMIVVPADTPGITIKRFLPVFGYDDAPHGHMEIELKNVRVPVSNILLGEGRGFEIAQGRLGPGRIHHCMRSIGVAERALELMCKRSLERVAFGKQIARQGVTQERIAEARCDIEMARLLTLKAAYMMDTVGNKIAKAEIAMIKVVAPNVALRVIDWAIQVHGAAGVSGDFPLANWWAHQRTLRLADGPDEVHRNAIAKLELAKHMKLNPDSIQMPITRGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="95" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LPV2</accession>
    <name>Q1LPV2_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="10">NADH:ubiquinone oxidoreductase, membrane subunit L</fullName>
            <ecNumber evidence="10">1.6.99.5</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">nuoL</name>
        <name evidence="10" type="ordered locus">Rmet_0938</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 5">Membrane</location>
            <topology evidence="1 5">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="1.6.99.5" evidence="10"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07824.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008643359.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0938"/>
    <dbReference type="EnsemblBacteria" id="ABF07824">
        <property type="protein sequence ID" value="ABF07824"/>
        <property type="gene ID" value="Rmet_0938"/>
    </dbReference>
    <dbReference type="GeneID" id="60825425"/>
    <dbReference type="KEGG" id="rme:Rmet_0938"/>
    <dbReference type="eggNOG" id="COG1009">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007100_6_0_4"/>
    <dbReference type="OMA" id="LIGFWQH"/>
    <dbReference type="OrthoDB" id="1274678at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008137">
        <property type="term" value="F:NADH dehydrogenase (ubiquinone) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042773">
        <property type="term" value="P:ATP synthesis coupled electron transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="NADHpl_OxRdtase_5_subgr"/>
    </dbReference>
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        <property type="entry name" value="ND/Mrp_mem"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003945">
        <property type="entry name" value="NU5C-like"/>
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        <property type="entry name" value="Proton_antipo_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42829">
        <property type="entry name" value="PTHR42829"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00361">
        <property type="entry name" value="Proton_antipo_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00662">
        <property type="entry name" value="Proton_antipo_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01974">
        <property type="entry name" value="NDH_I_L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="10" id="KW-0830">Ubiquinone</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="12"/>
            <end position="32"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="44"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="98"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="130"/>
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        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="153"/>
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        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
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    <feature type="transmembrane region" description="Helical" evidence="7">
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            <end position="247"/>
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    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
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        </location>
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    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="298"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="326"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="354"/>
            <end position="373"/>
        </location>
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    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
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        </location>
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    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="434"/>
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        <location>
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        </location>
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    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
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        <location>
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        <location>
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        </location>
    </feature>
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        <location>
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        </location>
    </feature>
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        <location>
            <begin position="468"/>
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        </location>
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        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
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    </evidence>
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        <source>
            <dbReference type="RuleBase" id="RU000320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
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        <source>
            <dbReference type="Pfam" id="PF00361"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF00662"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ABF07824.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="692" mass="75600" checksum="DC73AED6847C86BE" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="61" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LQ54</accession>
    <name>Q1LQ54_CUPMC</name>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name evidence="2" type="ordered locus">Rmet_0836</name>
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        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
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    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
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    </dbReference>
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            <begin position="1"/>
            <end position="20"/>
        </location>
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        <location>
            <begin position="21"/>
            <end position="197"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF07722.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="197" mass="22267" checksum="00085B63F6F02769" modified="2006-05-30" version="1">MSSRLSLPGTVLLSAVCIAAAGLSGCVVEPQRHVERVEVQHITQAPPAPIVEEIPPAPYVDAYWIPGHWAWKGGRYAWNGGHWEQARVNMVYQRAHWSNEHGEWVFHPGRWVELVQQARVAPIVVNVPPPMPRVEVMPPPPGPGHVWIGGYWRWSGGRHEWVAGQWEARRDSAFWVPGHWYRNGGGWAFAGGVWQQY</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="69" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LQ77</accession>
    <name>Q1LQ77_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
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        <name evidence="1" type="ordered locus">Rmet_0813</name>
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            <taxon>Bacteria</taxon>
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    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
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                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF07699.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="182" mass="20560" checksum="03B2FC5047E63028" modified="2006-05-30" version="1">MALKSTIFKADLSVSDMDRPYYGSHTLTIAQHPSENDARMMVRLLAFACEATETLTFTRGLDEPDEPDLWDKTLTGDISHWIDLGQPDETRLKRAAARADHVTVYTYQSASAREWWKGMSGKASKLRNVTIYNVPSDAVEALARMAQRAMRLSVTIQDGDIWVSDDDHNVQITREVLQRADD</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LQ96</accession>
    <name>Q1LQ96_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized ABC-type transport system, periplasmic component/surface lipoprotein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Rmet_0794</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the BMP lipoprotein family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07680.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515631.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0794"/>
    <dbReference type="EnsemblBacteria" id="ABF07680">
        <property type="protein sequence ID" value="ABF07680"/>
        <property type="gene ID" value="Rmet_0794"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0794"/>
    <dbReference type="eggNOG" id="COG1744">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038813_2_0_4"/>
    <dbReference type="OMA" id="PYTNMPD"/>
    <dbReference type="OrthoDB" id="603910at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028082">
        <property type="entry name" value="Peripla_BP_I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003760">
        <property type="entry name" value="PnrA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006311">
        <property type="entry name" value="TAT_signal"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02608">
        <property type="entry name" value="Bmp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53822">
        <property type="entry name" value="SSF53822"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51318">
        <property type="entry name" value="TAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193628" evidence="3">
        <location>
            <begin position="22"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="domain" description="Bmp" evidence="4">
        <location>
            <begin position="50"/>
            <end position="334"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008610"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF02608"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABF07680.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="380" mass="40962" checksum="159534BFD6CA9E07" modified="2006-05-30" version="1">MIVTRRTTLAALAATAVLALAGCGKKEAEKPAETAAPASAPAAAAAEPLKVAFVYIGPVGDAGWTYAHDAGRKAVEAKFGDKVKTTFVENVPESAADAERVFRDLASQGNKLIFGTTFGYMESMLKVAKEFPDVKFEHATGFKTAENLAQYDVRTYEGAYLAGVVAGKMSKTGKMGVVASVPIPEVIRNIDSFTLGARSVNPKATVKVVWVNKWFDPGKEREAATTLIGQGVDMLMQNTDSAAVVQTAQEKGVYAFGWDSDMSKFGEKAHLAASVISWGVYYNKVVEDVLNNQWKPNTTWWGLKEGMIDLKGFNTVVPDDVKALVEERKKGVIDGTAPIWKGPLKDNAGKEQLTKDQVADDNFLHNIKFYVDGVEGNVPG</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LQR4</accession>
    <name>Q1LQR4_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Rhs element Vgr protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Rmet_0626</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07512.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515483.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0626"/>
    <dbReference type="EnsemblBacteria" id="ABF07512">
        <property type="protein sequence ID" value="ABF07512"/>
        <property type="gene ID" value="Rmet_0626"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0626"/>
    <dbReference type="eggNOG" id="COG3501">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG4253">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004121_4_0_4"/>
    <dbReference type="OMA" id="DSWGAIR"/>
    <dbReference type="OrthoDB" id="1880900at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.230">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028244">
        <property type="entry name" value="T6SS_Rhs_Vgr_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017847">
        <property type="entry name" value="T6SS_RhsGE_Vgr_subset"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006533">
        <property type="entry name" value="T6SS_Vgr_RhsGE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037026">
        <property type="entry name" value="Vgr_OB-fold_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018769">
        <property type="entry name" value="VgrG2_C_DUF2345"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10106">
        <property type="entry name" value="DUF2345"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13296">
        <property type="entry name" value="T6SS_Vgr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01646">
        <property type="entry name" value="vgr_GE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03361">
        <property type="entry name" value="VI_Rhs_Vgr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="T6SS_Vgr" evidence="3">
        <location>
            <begin position="489"/>
            <end position="590"/>
        </location>
    </feature>
    <feature type="domain" description="DUF2345" evidence="2">
        <location>
            <begin position="609"/>
            <end position="755"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="576"/>
            <end position="617"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF10106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF13296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF07512.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="889" mass="97532" checksum="9E80C25F9C5BBC96" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="67" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LR97</accession>
    <name>Q1LR97_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF45 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_0443</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07329.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515317.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0443"/>
    <dbReference type="EnsemblBacteria" id="ABF07329">
        <property type="protein sequence ID" value="ABF07329"/>
        <property type="gene ID" value="Rmet_0443"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0443"/>
    <dbReference type="eggNOG" id="COG1451">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_065947_2_0_4"/>
    <dbReference type="OMA" id="RLNWRLM"/>
    <dbReference type="OrthoDB" id="1301886at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002725">
        <property type="entry name" value="YgjP-like_metallopeptidase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01863">
        <property type="entry name" value="DUF45"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF45" evidence="2">
        <location>
            <begin position="75"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01863"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF07329.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="292" mass="33114" checksum="00DAF1CA050FA741" modified="2006-05-30" version="1">MKLLRPAPETPGAQLELPLAEALPPVTPEQQDLSPTSAPQTPETASPWPPALPNGRRLQVGERTVHYALKRSSRRTIGFTVDDRGLSITAPRWVTLADVEAAILEKQRWIFNKLGEWRHREARRILPTVQWRDGASLPFLGQPITLQLESPAGVLMFNAELRMLHLALPAHADEQQIKDRVQGWMQNQARRLLAERLDIYAAKLGVRHTGFALTSAATRWGSCTADGKIRLNWRLMHFPLSMIDYVAAHELAHLKEMNHGPRFWETVESIFPEFRDARAQLRAHPPELLPTF</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="49" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LS05</accession>
    <name>Q1LS05_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_0185</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07071.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0185"/>
    <dbReference type="EnsemblBacteria" id="ABF07071">
        <property type="protein sequence ID" value="ABF07071"/>
        <property type="gene ID" value="Rmet_0185"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0185"/>
    <dbReference type="eggNOG" id="ENOG5032RT1">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_061560_1_0_4"/>
    <dbReference type="OMA" id="IANRAHW"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF07071.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="359" mass="40778" checksum="B65CE671F85E4403" modified="2006-05-30" version="1">MSVSENKLENPCLGAPRPAVHRTGWPRPNPCHAWAGGRSATPGNMRADSSMPPRTDLARSRRVTVVHNWSRRVFVVITTLTFVAILGGCNAMKLGYEQGDRLAYWWVDRYVDVSDAQEVPTRDAIARFFAWHRRSQLPEVAKVLARVKGEVAGPVDIAMIRQAQQDSQRLGRQAYENTIPDLADLMLTLSPDQIKRMETKFAESNTKYRKQFLGSDADARTDARFDKIMDYTKLVYGSFSRDQEKAIRDAMGPYMLNADARYSERVRRQQEWVALAREISTTRPPKAQAEEMLRHYADDWQRPATGQQRSTDAGINLTVTIANLTTPEQKAHAVKRFQGWIEDVQALMREPGRSTRASQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LSC7</accession>
    <name>Q1LSC7_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Putative allophanate hydrolase subunit 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Rmet_0063</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF06949.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011514988.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0063"/>
    <dbReference type="EnsemblBacteria" id="ABF06949">
        <property type="protein sequence ID" value="ABF06949"/>
        <property type="gene ID" value="Rmet_0063"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0063"/>
    <dbReference type="eggNOG" id="COG1984">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028967_0_3_4"/>
    <dbReference type="OMA" id="QDLGRSH"/>
    <dbReference type="OrthoDB" id="909676at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.100.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003778">
        <property type="entry name" value="CT_A_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029000">
        <property type="entry name" value="Cyclophilin-like_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02626">
        <property type="entry name" value="CT_A_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00797">
        <property type="entry name" value="AHS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50891">
        <property type="entry name" value="SSF50891"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00724">
        <property type="entry name" value="urea_amlyse_rel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AHS2" evidence="4">
        <location>
            <begin position="23"/>
            <end position="310"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00797"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABF06949.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="339" mass="36224" checksum="30AF6DD1FCA57167" modified="2006-05-30" version="1">MIEIIRPGAQASVQDLGRVGFRRFGVGRSGAADDLALRVGNRLLGNDPGAAAIEFTLGRAAVRFEADMRVALTGAECSANLDGVPVWSWHAFDVRRGETLTLPSPRGGTRTYLCVAGGIDVPLVMNSRSTDLKSGFGGFEGRVLREGDRLPVGRPGIEQGQDWVGVAAPGWALPGQDGGNAIAIRLLPGPEYADFEPASQAALWQSEWTITPQSNRMGLRLQGPALARRAERSADLLSHGVVPGVMQVPPSGQPIALMSDAQTTGGYPKIGTVIGADLWRLAQVPLGATVRFRQVTLEEAAAAQAEVDRYLRQIDQALAWQRDGMQIAARRRATTRFVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LSD5</accession>
    <name>Q1LSD5_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Peptidoglycan glycosyltransferase MrdB</fullName>
            <shortName evidence="6">PGT</shortName>
            <ecNumber evidence="6">2.4.1.129</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Cell elongation protein RodA</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">Cell wall polymerase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">Peptidoglycan polymerase</fullName>
            <shortName evidence="6">PG polymerase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6 7" type="primary">mrdB</name>
        <name evidence="6" type="synonym">rodA</name>
        <name evidence="7" type="ordered locus">Rmet_0055</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Peptidoglycan polymerase that is essential for cell wall elongation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>[GlcNAc-(1-&gt;4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)](n)-di-trans,octa-cis-undecaprenyl diphosphate + beta-D-GlcNAc-(1-&gt;4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-di-trans,octa-cis-undecaprenyl diphosphate = [GlcNAc-(1-&gt;4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)](n+1)-di-trans-octa-cis-undecaprenyl diphosphate + di-trans,octa-cis-undecaprenyl diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:23708"/>
            <dbReference type="Rhea" id="RHEA-COMP:9602"/>
            <dbReference type="Rhea" id="RHEA-COMP:9603"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:58405"/>
            <dbReference type="ChEBI" id="CHEBI:60033"/>
            <dbReference type="ChEBI" id="CHEBI:78435"/>
            <dbReference type="EC" id="2.4.1.129"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="6">Cell wall biogenesis; peptidoglycan biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cell inner membrane</location>
            <topology evidence="6">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the SEDS family. MrdB/RodA subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.129" evidence="6"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF06941.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011514980.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0055"/>
    <dbReference type="EnsemblBacteria" id="ABF06941">
        <property type="protein sequence ID" value="ABF06941"/>
        <property type="gene ID" value="Rmet_0055"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0055"/>
    <dbReference type="eggNOG" id="COG0772">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029243_2_2_4"/>
    <dbReference type="OMA" id="IFCTVGE"/>
    <dbReference type="OrthoDB" id="1133883at2"/>
    <dbReference type="UniPathway" id="UPA00219"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005887">
        <property type="term" value="C:integral component of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008955">
        <property type="term" value="F:peptidoglycan glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009252">
        <property type="term" value="P:peptidoglycan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008360">
        <property type="term" value="P:regulation of cell shape"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02079">
        <property type="entry name" value="PGT_RodA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018365">
        <property type="entry name" value="Cell_cycle_FtsW-rel_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001182">
        <property type="entry name" value="FtsW/RodA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011923">
        <property type="entry name" value="RodA/MrdB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30474">
        <property type="entry name" value="PTHR30474"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30474:SF1">
        <property type="entry name" value="PTHR30474:SF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01098">
        <property type="entry name" value="FTSW_RODA_SPOVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02210">
        <property type="entry name" value="rodA_shape"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00428">
        <property type="entry name" value="FTSW_RODA_SPOVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="3 6" id="KW-0133">Cell shape</keyword>
    <keyword evidence="6" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="6" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0573">Peptidoglycan synthesis</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="21"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="51"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="75"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="135"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="160"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="184"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="284"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="317"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="350"/>
            <end position="371"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02079"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABF06941.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="380" mass="41566" checksum="155836EC02575D8D" modified="2006-05-30" version="1">MDRRRVVSIIKSALTGFDKPLALIVFLLFATGIVALYSAAIDMPGRVEDQLRNILLSYVVMLVIAYMPTQLLMRIAVPLYTVGVALLIAVAMFGLIRKGARRWLNVGMVIQPSEIMKIAMPLMLAWYFQKREGVIKWFDFVVALIMLGIPVGLIAKQPDLGTALLVLAAGIYVIYFAGLTWKIILPILGAGVVVITLIVTYQNQICAPGVNWPILHDYQQHRVCTLLDPTTDPLGKGFHTIQSIIAIGSGGVTGKGWLKGTQTHLEFIPEKHTDFIFAVFSEEFGLIGNAVLLVLYLLLIFRGLYIAANAPTLFSRLLAGSITLIFFTYAFVNMGMVSGILPVVGVPLPLMSYGGTALVTLGMGIGILMSISRQKRLIQT</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LSF6</accession>
    <name>Q1LSF6_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Septum site-determining protein MinD</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Cell division inhibitor MinD</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">minD</name>
        <name evidence="11" type="ordered locus">Rmet_0034</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">ATPase required for the correct placement of the division site. Cell division inhibitors MinC and MinD act in concert to form an inhibitor capable of blocking formation of the polar Z ring septums. Rapidly oscillates between the poles of the cell to destabilize FtsZ filaments that have formed before they mature into polar Z rings.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Interacts with MinC and FtsZ.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the ParA family. MinD subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF06920.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008645118.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0034"/>
    <dbReference type="EnsemblBacteria" id="ABF06920">
        <property type="protein sequence ID" value="ABF06920"/>
        <property type="gene ID" value="Rmet_0034"/>
    </dbReference>
    <dbReference type="GeneID" id="60822753"/>
    <dbReference type="KEGG" id="rme:Rmet_0034"/>
    <dbReference type="eggNOG" id="COG2894">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037612_0_1_4"/>
    <dbReference type="OMA" id="CESAKAY"/>
    <dbReference type="OrthoDB" id="1631847at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025669">
        <property type="entry name" value="AAA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010223">
        <property type="entry name" value="MinD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025501">
        <property type="entry name" value="MinD_FleN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13614">
        <property type="entry name" value="AAA_31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF003092">
        <property type="entry name" value="MinD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01968">
        <property type="entry name" value="minD_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA_31" evidence="10">
        <location>
            <begin position="3"/>
            <end position="162"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011626"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00016887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00025436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00032845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF13614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ABF06920.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="270" mass="29539" checksum="457BAA6439996A20" modified="2006-05-30" version="1">MAKIIVVTSGKGGVGKTTTSASFAAGLALRGHKTAVIDFDVGLRNLDLIMGCERRVVYDLINVVQGEANLHQALIKDKKCENLFILPASQTRDKEALTREGVEKVINDLKEMGFEYIICDSPAGIETGALMAMYFADEALIVTNPEVSSVRDSDRILGILSSKTKRASEGEPIKEHLLITRYNPKRVHGGEMLSLTDIQEILRIKLIGVVPESEAVLHASNQGTPAIHLEGTDVADAYGDIVDRFLGKDKPLRFTDYQKPGLFSRIFGNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="69" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LSH1</accession>
    <name>Q1LSH1_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative beta-lactamase-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_0019</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF06905.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0019"/>
    <dbReference type="EnsemblBacteria" id="ABF06905">
        <property type="protein sequence ID" value="ABF06905"/>
        <property type="gene ID" value="Rmet_0019"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0019"/>
    <dbReference type="eggNOG" id="COG0426">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_066633_0_0_4"/>
    <dbReference type="OMA" id="CGIDLMT"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001279">
        <property type="entry name" value="Metallo-B-lactamas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045761">
        <property type="entry name" value="ODP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19583">
        <property type="entry name" value="ODP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00849">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="SSF56281"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Lactamase_B" evidence="1">
        <location>
            <begin position="26"/>
            <end position="218"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF06905.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="257" mass="28492" checksum="28F965A40AF409F8" modified="2006-05-30" version="1">MLYEAGGHVCLAFTDLVDEGQGEVVQSNQFLVVDNGHAALIDPGGNMTYSELYLTISRYFPPKQLDYVLASHADPDIVASVGRWLTSSDSRVLISQVWARFLPHFCQAGKTAGRIVTIPDGGMVIPLGNCQLIAVPAHFLHSEGNFQFYDPVSKILFSGDLGATMTSGTEAGTVVTDFDAHARRMLAFHRRYMSGNRACRLWAAMARTMDIEWIVPQHGPSFRGREMVNRFIDWIDQLQCGLDLLEPAHYRVPSMLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="99" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LSI8</accession>
    <name>Q1LSI8_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 10">Beta sliding clamp</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">dnaN</name>
        <name evidence="14" type="ordered locus">Rmet_0002</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Confers DNA tethering and processivity to DNA polymerases and other proteins. Acts as a clamp, forming a ring around DNA (a reaction catalyzed by the clamp-loading complex) which diffuses in an ATP-independent manner freely and bidirectionally along dsDNA. Initially characterized for its ability to contact the catalytic subunit of DNA polymerase III (Pol III), a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria; Pol III exhibits 3'-5' exonuclease proofreading activity. The beta chain is required for initiation of replication as well as for processivity of DNA replication.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Forms a ring-shaped head-to-tail homodimer around DNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the beta sliding clamp family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF06888.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008650852.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0002"/>
    <dbReference type="EnsemblBacteria" id="ABF06888">
        <property type="protein sequence ID" value="ABF06888"/>
        <property type="gene ID" value="Rmet_0002"/>
    </dbReference>
    <dbReference type="GeneID" id="60822724"/>
    <dbReference type="KEGG" id="rme:Rmet_0002"/>
    <dbReference type="eggNOG" id="COG0592">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038149_4_2_4"/>
    <dbReference type="OMA" id="YLIMPVR"/>
    <dbReference type="OrthoDB" id="1040142at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009360">
        <property type="term" value="C:DNA polymerase III complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008408">
        <property type="term" value="F:3'-5' exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003887">
        <property type="term" value="F:DNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00140">
        <property type="entry name" value="beta_clamp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001001">
        <property type="entry name" value="DNA_polIII_beta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022635">
        <property type="entry name" value="DNA_polIII_beta_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022637">
        <property type="entry name" value="DNA_polIII_beta_cen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022634">
        <property type="entry name" value="DNA_polIII_beta_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30478">
        <property type="entry name" value="PTHR30478"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00712">
        <property type="entry name" value="DNA_pol3_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02767">
        <property type="entry name" value="DNA_pol3_beta_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02768">
        <property type="entry name" value="DNA_pol3_beta_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000804">
        <property type="entry name" value="DNA_pol_III_b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00480">
        <property type="entry name" value="POL3Bc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00663">
        <property type="entry name" value="dnan"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 10" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 10" id="KW-0235">DNA replication</keyword>
    <keyword evidence="9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8 10" id="KW-0239">DNA-directed DNA polymerase</keyword>
    <keyword evidence="6 10 14" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 10 14" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="DNA_pol3_beta" evidence="11">
        <location>
            <begin position="7"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="domain" description="DNA_pol3_beta_2" evidence="12">
        <location>
            <begin position="131"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="domain" description="DNA_pol3_beta_3" evidence="13">
        <location>
            <begin position="251"/>
            <end position="370"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010752"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00021035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRNR" id="PIRNR000804"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00712"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF02767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF02768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ABF06888.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="371" mass="41034" checksum="FAA88897E26191AF" modified="2006-05-30" version="1">MQLVKTSRDNLLRPLQIVSGIVERRHTLPILANLLIRKSGSNVSFLSTDIEIQITTHAECGVGSDSVATTVAARKLLDILRAMPDGDVALSLNDKRMTVQSGKSRFALQTLAAEEFPTVAEAAEFNASVSLPQKTFKHLLAMVHFAMAQQDIRYYLNGMLLVVDGKKVMAVATDGHRLAYCGVELENEATGVGSRQEVIIPRKTILELQRLLEDTDDPVQVQLAANQVKFTFANIELISKLVEGKFPDFQRVIPKGYKNAFAIDRVQLQQALQRTAILTTDKFKGVRCILDTHVLKISSTNADQEEAQEELELDYSGDALDIGFNVTYLLDVLANLKSEQVQVSLGDSNSSALITVPEDDNFKYVVMPMRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-04-26" modified="2022-08-03" version="106" xmlns="http://uniprot.org/uniprot">
    <accession>Q58AI7</accession>
    <name>Q58AI7_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="11">TnpA Transposase Tn4380 mercury transposon Tn3 family</fullName>
        </submittedName>
        <submittedName>
            <fullName evidence="9">Transposase Tn6050</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">tnpA</name>
        <name evidence="9" type="ordered locus">Rmet_4474</name>
        <name evidence="10" type="ordered locus">Rmet_4598</name>
        <name evidence="11" type="ordered locus">Rmet_6179</name>
    </gene>
    <organism evidence="9 12">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="9">
        <name>megaplasmid</name>
    </geneLocation>
    <geneLocation type="plasmid" evidence="12">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <geneLocation type="plasmid" evidence="11 12">
        <name>pMOL30</name>
    </geneLocation>
    <reference evidence="9" key="1">
        <citation type="submission" date="2006-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of the chromosome of Ralstonia metallidurans CH34.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Copeland A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Barry K."/>
                <person name="Detter J.C."/>
                <person name="Glavina del Rio T."/>
                <person name="Hammon N."/>
                <person name="Israni S."/>
                <person name="Dalin E."/>
                <person name="Tice H."/>
                <person name="Martinez M."/>
                <person name="Goltsman E."/>
                <person name="Pitluck S."/>
                <person name="Schmutz J."/>
                <person name="Larimer F."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Kim E."/>
                <person name="Mergeay M."/>
                <person name="Benotmane M.A."/>
                <person name="Vallaeys T."/>
                <person name="Michaux A."/>
                <person name="Monchy S."/>
                <person name="Dunn J."/>
                <person name="McCorkle S."/>
                <person name="Taghavi S."/>
                <person name="van der Lelie D."/>
                <person name="Richardson P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">CH34</strain>
            <plasmid evidence="9">megaplasmid</plasmid>
            <plasmid evidence="11">pMOL30</plasmid>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="12">Plasmid megaplasmid CH34</plasmid>
            <plasmid evidence="12">Plasmid pMOL30</plasmid>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2010-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Benotmane R."/>
                <person name="Lapidus A."/>
                <person name="McCorkle S."/>
                <person name="Monchy S."/>
                <person name="Taghavi S."/>
                <person name="van der Lelie N."/>
                <person name="Dunn J."/>
                <person name="Leys N."/>
                <person name="Mergeay M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">CH34</strain>
            <plasmid evidence="9">megaplasmid</plasmid>
            <plasmid evidence="11">pMOL30</plasmid>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the transposase 7 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11339.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11460.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP000354">
        <property type="protein sequence ID" value="ABF13038.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_001138070.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_145631.1">
        <property type="nucleotide sequence ID" value="NC_006466.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4474"/>
    <dbReference type="EnsemblBacteria" id="ABF11339">
        <property type="protein sequence ID" value="ABF11339"/>
        <property type="gene ID" value="Rmet_4474"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF11460">
        <property type="protein sequence ID" value="ABF11460"/>
        <property type="gene ID" value="Rmet_4598"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF13038">
        <property type="protein sequence ID" value="ABF13038"/>
        <property type="gene ID" value="Rmet_6179"/>
    </dbReference>
    <dbReference type="GeneID" id="61089180"/>
    <dbReference type="KEGG" id="rme:Rmet_4474"/>
    <dbReference type="KEGG" id="rme:Rmet_4598"/>
    <dbReference type="KEGG" id="rme:Rmet_6179"/>
    <dbReference type="eggNOG" id="COG4644">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009098_1_0_4"/>
    <dbReference type="OMA" id="VIHYISK"/>
    <dbReference type="OrthoDB" id="45599at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid pMOL30"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004803">
        <property type="term" value="F:transposase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006313">
        <property type="term" value="P:transposition, DNA-mediated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025296">
        <property type="entry name" value="DUF4158"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002513">
        <property type="entry name" value="Tn3_Tnp_DDE_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01526">
        <property type="entry name" value="DDE_Tnp_Tn3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13700">
        <property type="entry name" value="DUF4158"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="9" id="KW-0614">Plasmid</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0814">Transposable element</keyword>
    <keyword evidence="3" id="KW-0815">Transposition</keyword>
    <feature type="domain" description="DUF4158" evidence="8">
        <location>
            <begin position="6"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="domain" description="DDE_Tnp_Tn3" evidence="7">
        <location>
            <begin position="580"/>
            <end position="966"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="672"/>
            <end position="697"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009402"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022578"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01526"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF13700"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ABF11339.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ABF11460.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ABF13038.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="988" mass="111067" checksum="C5CBA04A880A6B6F" modified="2005-04-26" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2005-01-04" modified="2021-06-02" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>Q5NUW7</accession>
    <name>Q5NUW7_CUPMC</name>
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        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_6238</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="1 2">
        <name>pMOL28</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid pMOL28</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000355">
        <property type="protein sequence ID" value="ABF13097.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
    <dbReference type="RefSeq" id="YP_161683.1">
        <property type="nucleotide sequence ID" value="NC_006525.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF13097">
        <property type="protein sequence ID" value="ABF13097"/>
        <property type="gene ID" value="Rmet_6238"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6238"/>
    <dbReference type="HOGENOM" id="CLU_061513_0_0_4"/>
    <dbReference type="OMA" id="QWAEPAY"/>
    <dbReference type="OrthoDB" id="1481060at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid pMOL28"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022283">
        <property type="entry name" value="PRTRC_protein-F"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14456">
        <property type="entry name" value="alpha-hel2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03742">
        <property type="entry name" value="PRTRC_F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF13097.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="379" mass="42020" checksum="A411D834BDA6EF8C" modified="2005-01-04" version="1">MLFDPRSFVPALDGGQPGWSFARQHPAARHRPSHGFLTLPAIAAETPGRAFLSFGDEPDALELARAQFETGVLRASDVVNPTSAADAFAQAMFAWLAARMPTCRRLNFSFSLVDLNAAKDQLMQFGWDDQVDASLYLAIDLPGDEVYFIGKARADALRAVHPYLLYTAMSLINLASSKSLHLRTPDVLLDLFARWHWEYDCTLANDDDAREFLKNGCGMDEGDIARYLPSAVRPELAPDDVLPPFCHAYPESRKLKTVGSRKLYELARSQHGWLKDVCVALAELNLAVKRQRDRSAVADSQWAEPAHSAATLAYAESDYVTQVLDDLYDGYANSGDATLFQCFIPIAVEPKAIRQQFEDLSGMFKIIAALDRVLTLISD</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-01-04" modified="2021-09-29" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>Q5NV36</accession>
    <name>Q5NV36_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Rmet_6286</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="6 7">
        <name>pMOL28</name>
    </geneLocation>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="7">Plasmid pMOL28</plasmid>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0057 (PMP3) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000355">
        <property type="protein sequence ID" value="ABF13145.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011239914.1">
        <property type="nucleotide sequence ID" value="NC_007972.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_161654.1">
        <property type="nucleotide sequence ID" value="NC_006525.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF13145">
        <property type="protein sequence ID" value="ABF13145"/>
        <property type="gene ID" value="Rmet_6286"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6286"/>
    <dbReference type="HOGENOM" id="CLU_172373_2_0_4"/>
    <dbReference type="OMA" id="QLTLIGW"/>
    <dbReference type="OrthoDB" id="2055682at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid pMOL28"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000612">
        <property type="entry name" value="PMP3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01679">
        <property type="entry name" value="Pmp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0614">Plasmid</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="28"/>
            <end position="47"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABF13145.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="65" mass="7423" checksum="7D18803E0E4FDD06" modified="2005-01-04" version="1">MRLLLAILLPWFQFFTIGRPFAGIICLILQLTIIGWIPAAIWSTYALSQYKTDKKIQAALNSRGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-03-23" modified="2020-10-07" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>D3DXM9</accession>
    <name>D3DXM9_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_6432</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ADC45049.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_6432"/>
    <dbReference type="EnsemblBacteria" id="ADC45049">
        <property type="protein sequence ID" value="ADC45049"/>
        <property type="gene ID" value="Rmet_6432"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6432"/>
    <dbReference type="HOGENOM" id="CLU_3423055_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADC45049.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="23" mass="2908" checksum="3B06539305B18680" modified="2010-03-23" version="1">MHAFPDTSFFLIWLHFLRQEQPF</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-03-23" modified="2020-10-07" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>D3DXN1</accession>
    <name>D3DXN1_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_6434</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ADC45051.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_6434"/>
    <dbReference type="EnsemblBacteria" id="ADC45051">
        <property type="protein sequence ID" value="ADC45051"/>
        <property type="gene ID" value="Rmet_6434"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6434"/>
    <dbReference type="HOGENOM" id="CLU_2424762_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="23"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADC45051.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="91" mass="8969" checksum="144787DB26C9A8BE" modified="2010-03-23" version="1">MKNGAFGPRFLLATVRNAPASATAAPVHAGPHPNGAAHSRCAGGFPSGPAGGTSVVRHGPPPVDLSRNGDPVTRVSLGTLVADVLSGVRSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-03-23" modified="2020-10-07" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>D3DXS3</accession>
    <name>D3DXS3_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_6485</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ADC45093.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_6485"/>
    <dbReference type="EnsemblBacteria" id="ADC45093">
        <property type="protein sequence ID" value="ADC45093"/>
        <property type="gene ID" value="Rmet_6485"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6485"/>
    <dbReference type="HOGENOM" id="CLU_3423056_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADC45093.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="23" mass="2796" checksum="9BBD342FA3048D2D" modified="2010-03-23" version="1">MFAYFIVFFALWGVVRLADRWGM</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-03-23" modified="2020-10-07" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>D3DXS5</accession>
    <name>D3DXS5_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_6487</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ADC45095.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_6487"/>
    <dbReference type="EnsemblBacteria" id="ADC45095">
        <property type="protein sequence ID" value="ADC45095"/>
        <property type="gene ID" value="Rmet_6487"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6487"/>
    <dbReference type="HOGENOM" id="CLU_2957378_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADC45095.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="59" mass="6746" checksum="98D601A4A9AF9560" modified="2010-03-23" version="1">MKLSIVDCLSFGWRDVILILSRAPARGHLFRLFSDVIELPILQQTQGSRRNAPVKGLRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-03-23" modified="2020-10-07" version="21" xmlns="http://uniprot.org/uniprot">
    <accession>D3DXX4</accession>
    <name>D3DXX4_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_6537</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ADC45144.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADC45144">
        <property type="protein sequence ID" value="ADC45144"/>
        <property type="gene ID" value="Rmet_6537"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6537"/>
    <dbReference type="HOGENOM" id="CLU_3421142_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADC45144.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="24" mass="2820" checksum="FB8A1C236C7CFAFF" modified="2010-03-23" version="1">MEFGGLDETVDSENFGSEFRFIHR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-03-23" modified="2020-10-07" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>D3DY56</accession>
    <name>D3DY56_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_6625</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ADC45226.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_6625"/>
    <dbReference type="EnsemblBacteria" id="ADC45226">
        <property type="protein sequence ID" value="ADC45226"/>
        <property type="gene ID" value="Rmet_6625"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6625"/>
    <dbReference type="HOGENOM" id="CLU_2772947_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADC45226.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="69" mass="7540" checksum="8F7AAC709ED0D59E" modified="2010-03-23" version="1">MRPSDGRWIAYGYTPRQTLDCFSLFGCSRLRSSALAVAGSCRPCGASGARSSRFEMECYGKVATMSSWT</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-03-23" modified="2020-10-07" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>D3DYA7</accession>
    <name>D3DYA7_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_6694</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ADC45277.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_6694"/>
    <dbReference type="EnsemblBacteria" id="ADC45277">
        <property type="protein sequence ID" value="ADC45277"/>
        <property type="gene ID" value="Rmet_6694"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6694"/>
    <dbReference type="HOGENOM" id="CLU_218782_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADC45277.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="33" mass="3330" checksum="804182B92BA8DC11" modified="2010-03-23" version="1">MSLRDSALILGGWVMLALGSGALVSLVAKSLSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-03-23" modified="2020-02-26" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>D3DYI9</accession>
    <name>D3DYI9_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Conserved protein involved in resistance to copper</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">copO</name>
        <name evidence="3" type="ordered locus">Rmet_6382</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3 4">
        <name>pMOL30</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid pMOL30</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000354">
        <property type="protein sequence ID" value="ADC45359.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADC45359">
        <property type="protein sequence ID" value="ADC45359"/>
        <property type="gene ID" value="Rmet_6382"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6382"/>
    <dbReference type="HOGENOM" id="CLU_2882725_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid pMOL30"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021682">
        <property type="entry name" value="DUF2933"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11666">
        <property type="entry name" value="DUF2933"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="16"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="38"/>
            <end position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADC45359.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="63" mass="6998" checksum="47B25F19E37D77BF" modified="2010-03-23" version="1">MSIVALAFAASDWKRLASMLPFGLVLLCPLMHFHHAGRHRNSSSARGNEANEQRVGIQESHNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-03-23" modified="2020-10-07" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>D3DYJ3</accession>
    <name>D3DYJ3_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_6392</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="1 2">
        <name>pMOL28</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid pMOL28</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000355">
        <property type="protein sequence ID" value="ADC45363.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADC45363">
        <property type="protein sequence ID" value="ADC45363"/>
        <property type="gene ID" value="Rmet_6392"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6392"/>
    <dbReference type="HOGENOM" id="CLU_2719426_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid pMOL28"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADC45363.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="72" mass="8058" checksum="70CBE5BE9CF2715A" modified="2010-03-23" version="1">MLAWTHIDRRCAVVQANFSICRGGEGVIEASAETTNSGAVCWFLGGSRQDSLLDKKDYQECEKRYGREQSMP</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="53" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LAB4</accession>
    <name>Q1LAB4_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_6053</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="1 2">
        <name>pMOL30</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid pMOL30</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000354">
        <property type="protein sequence ID" value="ABF12912.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011514779.1">
        <property type="nucleotide sequence ID" value="NC_007971.2"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF12912">
        <property type="protein sequence ID" value="ABF12912"/>
        <property type="gene ID" value="Rmet_6053"/>
    </dbReference>
    <dbReference type="GeneID" id="60825079"/>
    <dbReference type="KEGG" id="rme:Rmet_6053"/>
    <dbReference type="HOGENOM" id="CLU_2384051_0_0_4"/>
    <dbReference type="OMA" id="GMASMGC"/>
    <dbReference type="OrthoDB" id="2169203at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid pMOL30"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF12912.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="94" mass="10011" checksum="CDADA13D22A695B0" modified="2006-05-30" version="1">MNKTAGETSLATTIGMASMGCIDSEGQPKCSKFVNASCSGMRAMTCMSNALQDYPEARAEILLAGLTVVSKSSKNILEIRKFVPRMEMAVQVTA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-12-12" modified="2022-08-03" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LB18</accession>
    <name>GLK_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Glucokinase</fullName>
            <ecNumber evidence="1">2.7.1.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Glucose kinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">glk</name>
        <name type="ordered locus">Rmet_5799</name>
    </gene>
    <organism>
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + D-glucose = ADP + D-glucose 6-phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17825"/>
            <dbReference type="ChEBI" id="CHEBI:4167"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61548"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.2"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the bacterial glucokinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.2" evidence="1"/>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12658.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011520194.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q1LB18"/>
    <dbReference type="SMR" id="Q1LB18"/>
    <dbReference type="STRING" id="266264.Rmet_5799"/>
    <dbReference type="EnsemblBacteria" id="ABF12658">
        <property type="protein sequence ID" value="ABF12658"/>
        <property type="gene ID" value="Rmet_5799"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5799"/>
    <dbReference type="eggNOG" id="COG0837">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_042582_1_0_4"/>
    <dbReference type="OMA" id="NNHWRLS"/>
    <dbReference type="OrthoDB" id="992687at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004340">
        <property type="term" value="F:glucokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005536">
        <property type="term" value="F:glucose binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006096">
        <property type="term" value="P:glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00524">
        <property type="entry name" value="Glucokinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003836">
        <property type="entry name" value="Glucokinase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02685">
        <property type="entry name" value="Glucokinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="SSF53067"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00749">
        <property type="entry name" value="glk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0324">Glycolysis</keyword>
    <keyword id="KW-0418">Kinase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0614">Plasmid</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000268781" description="Glucokinase">
        <location>
            <begin position="1"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="13"/>
            <end position="18"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00524"/>
        </source>
    </evidence>
    <sequence length="336" mass="35185" checksum="47721EFB63A9591E" modified="2006-05-30" version="1">MTDRAMSYPRILADVGGTNVRFAMETAPMRIGEITAYKVAEHASLEAAMRLYMLTRSGAARPRHAAIGLANPVTGDQVKLTNHNWAFSVEAMRRALDLDTLVAINDFTSLALALPYLPDASLVQVRDGTAVATAPRALIGPGTGLGVSGLIPAPGGAVALAGEGGHIEIMPVTDDEWIAWRAAHDQFGRVSAERLLSGMGLSHIHAALSAEMGTPLEVPLAPAQVTDGAMRAGDPVCRRAFDAFCGMLGSVAADVALVLGARGGVYLGGGIVPRFVDALRASTFAERFVAKGRMGSFLADVPVYVITAEYPALPGLARALADRLEADARRAETSQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="97" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LB24</accession>
    <name>Q1LB24_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Ubiquinol oxidase subunit 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">cyoA</name>
        <name evidence="15" type="ordered locus">Rmet_5793</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="16">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="16">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="11">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Periplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the cytochrome c oxidase subunit 2 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12652.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5793"/>
    <dbReference type="EnsemblBacteria" id="ABF12652">
        <property type="protein sequence ID" value="ABF12652"/>
        <property type="gene ID" value="Rmet_5793"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5793"/>
    <dbReference type="eggNOG" id="COG1622">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036876_6_1_4"/>
    <dbReference type="OMA" id="VVWGIPC"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042597">
        <property type="term" value="C:periplasmic space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070469">
        <property type="term" value="C:respirasome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009486">
        <property type="term" value="F:cytochrome bo3 ubiquinol oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004129">
        <property type="term" value="F:cytochrome-c oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016682">
        <property type="term" value="F:oxidoreductase activity, acting on diphenols and related substances as donors, oxygen as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04212">
        <property type="entry name" value="CuRO_UO_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.420">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045187">
        <property type="entry name" value="CcO_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002429">
        <property type="entry name" value="CcO_II-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010514">
        <property type="entry name" value="COX_ARM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008972">
        <property type="entry name" value="Cupredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034227">
        <property type="entry name" value="CuRO_UO_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011759">
        <property type="entry name" value="Cyt_c_oxidase_su2_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036257">
        <property type="entry name" value="Cyt_c_oxidase_su2_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006333">
        <property type="entry name" value="Cyt_o_ubiquinol_oxidase_su2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22888">
        <property type="entry name" value="PTHR22888"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00116">
        <property type="entry name" value="COX2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06481">
        <property type="entry name" value="COX_ARM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000292">
        <property type="entry name" value="Ubi_od_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49503">
        <property type="entry name" value="SSF49503"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81464">
        <property type="entry name" value="SSF81464"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01433">
        <property type="entry name" value="CyoA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50857">
        <property type="entry name" value="COX2_CUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50999">
        <property type="entry name" value="COX2_TM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 11" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8 11" id="KW-0249">Electron transport</keyword>
    <keyword evidence="10 11 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="15" id="KW-0614">Plasmid</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 11" id="KW-0679">Respiratory chain</keyword>
    <keyword evidence="7 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4 11" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="21"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="53"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="99"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="domain" description="COX2_TM" evidence="14">
        <location>
            <begin position="32"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="domain" description="COX2_CUA" evidence="13">
        <location>
            <begin position="135"/>
            <end position="247"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007866"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRNR" id="PIRNR000292"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="ABF12652.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="319" mass="35409" checksum="919D1B4B2E7E0150" modified="2006-05-30" version="1">MRERVTAPEHVRRRIPIPPKVRAWLYVAAPLILSGCSYDLLSPSGAVGVQTRNLIVLSTALMLLVVIPVIILTLVFAWRYRETNTKAEYAPRWSHSTKIEVVVWGIPCVIVALLGIIIWDTTHKLDPFRPLESKVAPIEVDVVALDWKWLFIYPQYGVASLNELPVPVGTPINFRITAESTMNALFIPKLGSMVYAMSGMQTKLHLIADETGVFDGRSAAYSGAGFSDMHFRTVATSREDFDAWIARARASGNVLDEQTYRALKQPSSKDPVTIYANVSPRLFQGVVDQYMASNGDICRADSPETLRAALPPLPDTVEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="102" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LBK9</accession>
    <name>Q1LBK9_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Ubiquinol oxidase subunit 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">cyoA</name>
        <name evidence="15" type="ordered locus">Rmet_5608</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="16">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="16">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="11">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Periplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the cytochrome c oxidase subunit 2 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12467.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011520015.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5608"/>
    <dbReference type="EnsemblBacteria" id="ABF12467">
        <property type="protein sequence ID" value="ABF12467"/>
        <property type="gene ID" value="Rmet_5608"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5608"/>
    <dbReference type="eggNOG" id="COG1622">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036876_0_0_4"/>
    <dbReference type="OMA" id="TAMNSFF"/>
    <dbReference type="OrthoDB" id="1654242at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042597">
        <property type="term" value="C:periplasmic space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070469">
        <property type="term" value="C:respirasome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009486">
        <property type="term" value="F:cytochrome bo3 ubiquinol oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004129">
        <property type="term" value="F:cytochrome-c oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016682">
        <property type="term" value="F:oxidoreductase activity, acting on diphenols and related substances as donors, oxygen as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04212">
        <property type="entry name" value="CuRO_UO_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.420">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045187">
        <property type="entry name" value="CcO_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002429">
        <property type="entry name" value="CcO_II-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010514">
        <property type="entry name" value="COX_ARM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008972">
        <property type="entry name" value="Cupredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034227">
        <property type="entry name" value="CuRO_UO_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011759">
        <property type="entry name" value="Cyt_c_oxidase_su2_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036257">
        <property type="entry name" value="Cyt_c_oxidase_su2_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006333">
        <property type="entry name" value="Cyt_o_ubiquinol_oxidase_su2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22888">
        <property type="entry name" value="PTHR22888"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00116">
        <property type="entry name" value="COX2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06481">
        <property type="entry name" value="COX_ARM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000292">
        <property type="entry name" value="Ubi_od_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49503">
        <property type="entry name" value="SSF49503"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81464">
        <property type="entry name" value="SSF81464"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01433">
        <property type="entry name" value="CyoA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50857">
        <property type="entry name" value="COX2_CUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50999">
        <property type="entry name" value="COX2_TM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 11" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8 11" id="KW-0249">Electron transport</keyword>
    <keyword evidence="10 11 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="15" id="KW-0614">Plasmid</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 11" id="KW-0679">Respiratory chain</keyword>
    <keyword evidence="7 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4 11" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="17"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="51"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="95"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="domain" description="COX2_TM" evidence="14">
        <location>
            <begin position="29"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="domain" description="COX2_CUA" evidence="13">
        <location>
            <begin position="141"/>
            <end position="253"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007866"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRNR" id="PIRNR000292"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="ABF12467.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="349" mass="38507" checksum="816BBDA5F2423679" modified="2006-05-30" version="1">MPRFDAAVTPIRVTGRLLLRFTAIALLASLAGCNAVLLSPSGDMAMRQRDLIIIATCLMLLIIVPVIFLTLLFAWRYRESAPDSPYNPEWDHSTVLELAIWAAPLLIIIALGAVTWVSTHQLDPYRPLTRLDEGRPVPTEVKPLTVEVVALDWKWLFLYPEQGIATVNELAAPVDRPIAFRITATSVMNAFFVPSLAGMVYAMPGMETKLHAVINKPGIYDGFSSNYSGAGFSHMRFKFHGMSSEDFDRWIAQAKSSGSTLSKDAYLKLAQPSEREPVQRYASVAPELYDQILNRCVDGGACMADTMALDRSRGKFDPSGEICTASNTPVDSPLFAPDVVLNDGRRLLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="102" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LCB8</accession>
    <name>Q1LCB8_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Transcriptional regulatory protein, sigma-54 interaction domain, HTH Fis-type</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Rmet_5349</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="8">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="8">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12208.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519753.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5349"/>
    <dbReference type="EnsemblBacteria" id="ABF12208">
        <property type="protein sequence ID" value="ABF12208"/>
        <property type="gene ID" value="Rmet_5349"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5349"/>
    <dbReference type="eggNOG" id="COG3829">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_8_10_4"/>
    <dbReference type="OrthoDB" id="123059at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00130">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000014">
        <property type="entry name" value="PAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035965">
        <property type="entry name" value="PAS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013656">
        <property type="entry name" value="PAS_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002078">
        <property type="entry name" value="Sigma_54_int"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025662">
        <property type="entry name" value="Sigma_54_int_dom_ATP-bd_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025943">
        <property type="entry name" value="Sigma_54_int_dom_ATP-bd_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025944">
        <property type="entry name" value="Sigma_54_int_dom_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08448">
        <property type="entry name" value="PAS_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00158">
        <property type="entry name" value="Sigma54_activat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55785">
        <property type="entry name" value="SSF55785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00675">
        <property type="entry name" value="SIGMA54_INTERACT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00676">
        <property type="entry name" value="SIGMA54_INTERACT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00688">
        <property type="entry name" value="SIGMA54_INTERACT_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50045">
        <property type="entry name" value="SIGMA54_INTERACT_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0614">Plasmid</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Sigma-54 factor interaction" evidence="6">
        <location>
            <begin position="134"/>
            <end position="361"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50045"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABF12208.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="441" mass="49120" checksum="07B281840BB66B0F" modified="2010-03-23" version="2">MDRPTTLQGLLSYLEQESQPTIVLDTDYRILAANTAYRRQFGAVERPYLGQPCYRVSHGYDVPCDQAGEHCPMRKAFETRGPDRVLHIHHTPRGPEHVNVELRPILDDNHQVVAYVERLNTVRSASARPSAEGLVGRSEAFNHALEAVQRVAPSSLPVLLLGESGTGKEMFARAVHEASDRASGPFVVVDCSGLTETLFESELFGYEKGAFTGAQARKPGLVETAEGGTLFLDEMGDVPLAMQVKLLRLIESGTYRRVGGVETLQSNFRLVAATHKPLDQMVAQGLFRQDLYFRIAAFPIHLPSLRDRLDDIALLAESILARGNRTKDPLRLSSEALARMRAHHWPGNIRELRNVLERARLYADDGVIRVKDLPPGMGEARVSSVRFVPPSAYRTQAPRQDDDEALRFHASHFTGTRRALAQSLGMSERTLYRRLKALGIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="98" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LCD7</accession>
    <name>Q1LCD7_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Membrane fusion protein (MFP-RND) heavy metal cation tricomponent efflux HmxB (CzcB-like)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">zneB</name>
        <name evidence="5" type="ordered locus">Rmet_5330</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="6">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="6">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="journal article" date="2010" name="Proc. Natl. Acad. Sci. U.S.A." volume="107" first="11038" last="11043">
            <title>Metal-induced conformational changes in ZneB suggest an active role of membrane fusion proteins in efflux resistance systems.</title>
            <authorList>
                <person name="De Angelis F."/>
                <person name="Lee J.K."/>
                <person name="O'Connell J.D."/>
                <person name="Miercke L.J."/>
                <person name="Verschueren K.H."/>
                <person name="Srinivasan V."/>
                <person name="Bauvois C."/>
                <person name="Govaerts C."/>
                <person name="Robbins R.A."/>
                <person name="Ruysschaert J.M."/>
                <person name="Stroud R.M."/>
                <person name="Vandenbussche G."/>
            </authorList>
            <dbReference type="PubMed" id="20534468"/>
            <dbReference type="DOI" id="10.1073/pnas.1003908107"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (2.80 ANGSTROMS) OF 37-385 IN COMPLEX WITH ZINC</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12189.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519732.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="PDB" id="3LNN">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.80 A"/>
        <property type="chains" value="A/B=37-385"/>
    </dbReference>
    <dbReference type="PDBsum" id="3LNN"/>
    <dbReference type="SMR" id="Q1LCD7"/>
    <dbReference type="STRING" id="266264.Rmet_5330"/>
    <dbReference type="TCDB" id="2.A.6.1.6">
        <property type="family name" value="the resistance-nodulation-cell division (rnd) superfamily"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF12189">
        <property type="protein sequence ID" value="ABF12189"/>
        <property type="gene ID" value="Rmet_5330"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5330"/>
    <dbReference type="eggNOG" id="COG0845">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018816_13_3_4"/>
    <dbReference type="OMA" id="PEREIGQ"/>
    <dbReference type="OrthoDB" id="1174564at2"/>
    <dbReference type="EvolutionaryTrace" id="Q1LCD7"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043602">
        <property type="entry name" value="CusB_dom_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032317">
        <property type="entry name" value="HlyD_D23"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006143">
        <property type="entry name" value="RND_pump_MFP"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00529">
        <property type="entry name" value="CusB_dom_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16576">
        <property type="entry name" value="HlyD_D23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01730">
        <property type="entry name" value="RND_mfp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword evidence="7" id="KW-0002">3D-structure</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0614">Plasmid</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="domain" description="CusB_dom_1" evidence="3">
        <location>
            <begin position="11"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="domain" description="HlyD_D23" evidence="4">
        <location>
            <begin position="77"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="256"/>
        </location>
        <ligand>
            <name>Zinc</name>
            <dbReference type="ChEBI" id="CHEBI:27363"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="320"/>
        </location>
        <ligand>
            <name>Zinc</name>
            <dbReference type="ChEBI" id="CHEBI:27363"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="364"/>
        </location>
        <ligand>
            <name>Zinc</name>
            <dbReference type="ChEBI" id="CHEBI:27363"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF16576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABF12189.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="7">
        <source>
            <dbReference type="PDB" id="3LNN"/>
        </source>
    </evidence>
    <sequence length="385" mass="41375" checksum="98905AA7FBF4F9AF" modified="2006-05-30" version="1">MKNKPAFPGRVVYWLAAAVILGLGGAGVWTMRAKAEQKRADPPVALRHEGERLVVPAESPLRRTLAVAPATRETVAAPFNLPAMIEADPAKLVKVLPPLAGRIVSLNKQLGDEVKAGDVLFTIDSADLAQANSDAAKARAAMTMARRNLDRQRELDKSEIAAKRDFEQAQSDYDQAASESQRADARLAQLGAKGGGTLQAGGGHILAVRSPINGRVVDLNAATGAYWNDTTASLMTVADLSHVFVTANAQEKDLGHVYVGQSATVKFDAYDDPQPGKVRYVGQILDADTRTTKVRMVFDNPDGRLRPGMFAQATFLSQPHEGIVVPMSAIVQSGFYTRAFVEVAPWQFEPRVIKLGAQIGDRMEVKSGLSAGDRVVVKEGVLLND</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LCG1</accession>
    <name>Q1LCG1_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Methyl-accepting chemotaxis sensory transducer</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">tar</name>
        <name evidence="6" type="ordered locus">Rmet_5306</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="7">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="7">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the methyl-accepting chemotaxis (MCP) protein family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12165.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5306"/>
    <dbReference type="EnsemblBacteria" id="ABF12165">
        <property type="protein sequence ID" value="ABF12165"/>
        <property type="gene ID" value="Rmet_5306"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5306"/>
    <dbReference type="eggNOG" id="COG0840">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_107_16_4"/>
    <dbReference type="OMA" id="TYTHERE"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004888">
        <property type="term" value="F:transmembrane signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006935">
        <property type="term" value="P:chemotaxis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004090">
        <property type="entry name" value="Chemotax_Me-accpt_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003660">
        <property type="entry name" value="HAMP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024478">
        <property type="entry name" value="HlyB_4HB_MCP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004089">
        <property type="entry name" value="MCPsignal_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12729">
        <property type="entry name" value="4HB_MCP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00672">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00015">
        <property type="entry name" value="MCPsignal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00260">
        <property type="entry name" value="CHEMTRNSDUCR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00304">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00283">
        <property type="entry name" value="MA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50111">
        <property type="entry name" value="CHEMOTAXIS_TRANSDUC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50885">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0614">Plasmid</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0807">Transducer</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="35"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="218"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="domain" description="HAMP" evidence="5">
        <location>
            <begin position="239"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="domain" description="Methyl-accepting transducer" evidence="4">
        <location>
            <begin position="296"/>
            <end position="525"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00029447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABF12165.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="565" mass="59690" checksum="412D8994CA3F3CDB" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LCG9</accession>
    <name>Q1LCG9_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Flagellar motor switch protein FliM</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">fliM</name>
        <name evidence="13" type="ordered locus">Rmet_5298</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="14">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="14">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">FliM is one of three proteins (FliG, FliN, FliM) that forms the rotor-mounted switch complex (C ring), located at the base of the basal body. This complex interacts with the CheY and CheZ chemotaxis proteins, in addition to contacting components of the motor that determine the direction of flagellar rotation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Bacterial flagellum basal body</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cell inner membrane</location>
            <topology evidence="3">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the FliM family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12157.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519703.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5298"/>
    <dbReference type="EnsemblBacteria" id="ABF12157">
        <property type="protein sequence ID" value="ABF12157"/>
        <property type="gene ID" value="Rmet_5298"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5298"/>
    <dbReference type="eggNOG" id="COG1868">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_052646_1_2_4"/>
    <dbReference type="OMA" id="NPQFTQI"/>
    <dbReference type="OrthoDB" id="1748746at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009425">
        <property type="term" value="C:bacterial-type flagellum basal body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003774">
        <property type="term" value="F:cytoskeletal motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071973">
        <property type="term" value="P:bacterial-type flagellum-dependent cell motility"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006935">
        <property type="term" value="P:chemotaxis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd17908">
        <property type="entry name" value="FliM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.330.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1550.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028976">
        <property type="entry name" value="CheC-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001689">
        <property type="entry name" value="Flag_FliM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001543">
        <property type="entry name" value="FliN-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036429">
        <property type="entry name" value="SpoA-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02154">
        <property type="entry name" value="FliM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01052">
        <property type="entry name" value="FliMN_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002888">
        <property type="entry name" value="FliM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00955">
        <property type="entry name" value="FLGMOTORFLIM"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101801">
        <property type="entry name" value="SSF101801"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103039">
        <property type="entry name" value="SSF103039"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01397">
        <property type="entry name" value="fliM_switch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0975">Bacterial flagellum</keyword>
    <keyword evidence="6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="13" id="KW-0966">Cell projection</keyword>
    <keyword evidence="7" id="KW-0145">Chemotaxis</keyword>
    <keyword evidence="13" id="KW-0969">Cilium</keyword>
    <keyword evidence="8" id="KW-0283">Flagellar rotation</keyword>
    <keyword evidence="13" id="KW-0282">Flagellum</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-0614">Plasmid</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FliMN_C" evidence="12">
        <location>
            <begin position="254"/>
            <end position="322"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00021898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022500"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022779"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023143"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00025044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF01052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ABF12157.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="336" mass="37851" checksum="1D52AC010D948E24" modified="2006-05-30" version="1">MAYDKFLSQDEVDELLKGVSGESDAPVASAPVADDGGVRPYNLANQERIVRGRLHTLEIINERFARALRTALFNFIRRGADISVGTVRIEKYGDFVRNLPVPTNLNLVHMKPLRGTALFVYDPNLVFLVVDNLFGGDGRFHTRVEGRDFTQTEQRIIRRMLDLTLSSYGQAWRTVHPIEFDYVRSEMHTKFANVAGPNDAVVTTAFHIELGAVGGQLHICIPFSMIEPLRDLLMNPLQDEVEVDKRWLGQLSNQLRAAEVELVAEFTHLRSTVAEVLAMREGDVLPIELPEHIFGKVNGVPVMQCGFGTMNGQYALRVERMLNYQDSDSNKETDHD</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LCR7</accession>
    <name>Q1LCR7_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative glyoxalase/bleomycin resistance protein/dihydroxybiphenyl dioxygenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_5200</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12059.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008645561.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5200"/>
    <dbReference type="EnsemblBacteria" id="ABF12059">
        <property type="protein sequence ID" value="ABF12059"/>
        <property type="gene ID" value="Rmet_5200"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5200"/>
    <dbReference type="eggNOG" id="COG0346">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1359468_0_0_4"/>
    <dbReference type="OMA" id="DWIGDQM"/>
    <dbReference type="OrthoDB" id="1984437at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051213">
        <property type="term" value="F:dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.180.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029068">
        <property type="entry name" value="Glyas_Bleomycin-R_OHBP_Dase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004360">
        <property type="entry name" value="Glyas_Fos-R_dOase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037523">
        <property type="entry name" value="VOC"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00903">
        <property type="entry name" value="Glyoxalase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54593">
        <property type="entry name" value="SSF54593"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51819">
        <property type="entry name" value="VOC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="VOC" evidence="2">
        <location>
            <begin position="8"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="171"/>
            <end position="201"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF12059.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="201" mass="22323" checksum="FBDD74ED3FB6E37A" modified="2006-05-30" version="1">MTPIRTRKLGHLVLMVRDLEASTRFYTEVLGLTVSDRIADQMVFLRAGEDHHDLALSRLPPDAPDRDTPPSYTRPGLEHFSYYVESLDEMQRAVEVARSHGVTIERGIGQHGPGGNWFLVFKDPDGNNVEIYTEMQQIPTGSEHTAQTWERNLESFDRHRLAHFVVPPAPGLLAAKQRSEQRPAPAEDDDAGKDTGGGAGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="106" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LD60</accession>
    <name>Q1LD60_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Putative metalloprotease/Xaa-Pro dipeptidase type M24</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Rmet_5054</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="9">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="9">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="5">Belongs to the peptidase M24B family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11916.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519466.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5054"/>
    <dbReference type="EnsemblBacteria" id="ABF11916">
        <property type="protein sequence ID" value="ABF11916"/>
        <property type="gene ID" value="Rmet_5054"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5054"/>
    <dbReference type="eggNOG" id="COG0006">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017266_4_1_4"/>
    <dbReference type="OMA" id="PYLNGAN"/>
    <dbReference type="OrthoDB" id="415910at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008237">
        <property type="term" value="F:metallopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.350.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029149">
        <property type="entry name" value="Creatin/AminoP/Spt16_NTD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036005">
        <property type="entry name" value="Creatinase/aminopeptidase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000587">
        <property type="entry name" value="Creatinase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000994">
        <property type="entry name" value="Pept_M24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001131">
        <property type="entry name" value="Peptidase_M24B_aminopep-P_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01321">
        <property type="entry name" value="Creatinase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00557">
        <property type="entry name" value="Peptidase_M24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53092">
        <property type="entry name" value="SSF53092"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55920">
        <property type="entry name" value="SSF55920"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00491">
        <property type="entry name" value="PROLINE_PEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 8" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="8" id="KW-0614">Plasmid</keyword>
    <keyword evidence="1 8" id="KW-0645">Protease</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Creatinase_N" evidence="7">
        <location>
            <begin position="12"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase_M24" evidence="6">
        <location>
            <begin position="168"/>
            <end position="377"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU000590"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01321"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ABF11916.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="397" mass="44085" checksum="CDBB986AAC1B169A" modified="2006-05-30" version="1">MPHLTKAVHDYRLAAMHRLMDRLRVDALVFGSPDFFQYATNFQLDVWPWERPVFVVVPRNGETFAVMNELSTNHMRFATEKGTVWLSDVTYYAEHPRVANRLALPSQLPEVVAALLAAKGLGMSRIAVDVQHPILTGAQRYLPEMTLRPAMAEMRSLRWVKHAEELQIMRDLAALTDWVQDRYRENIRPGRLVQELDHAMAALMVEEAARRFAGEDFEIMKCWTLTGAASASPHGDGAACGSRIAKGDTIVNLVLPRLNGLYVENERTWFCGQPSDEQARFYSVAARATDAAVAAAVVGRRVCDMDAAAQAVIESAGCGDYVFHRTGHAVGLMLHEYPEDMAFNTRPLLAGEVYSSEPGLYVYGLGGFRLDDTVVVGEVPEVIVKTPKTLSYATVSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="50" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LD72</accession>
    <name>Q1LD72_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_5042</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11904.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519455.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5042"/>
    <dbReference type="EnsemblBacteria" id="ABF11904">
        <property type="protein sequence ID" value="ABF11904"/>
        <property type="gene ID" value="Rmet_5042"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5042"/>
    <dbReference type="HOGENOM" id="CLU_2510302_0_0_4"/>
    <dbReference type="OrthoDB" id="2079614at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF11904.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="85" mass="9617" checksum="32EF9CA2942AD6DE" modified="2006-05-30" version="1">MTMKTEEQVQAEIAALRALQPQLPERARKAVDAALMVLEKGLSHDNVYDMFEEGTEEFEDAFAARMWREGAPGSESLSVLYRELI</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="103" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LDE9</accession>
    <name>Q1LDE9_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Two component transcriptional regulator, LuxR family, DNA binding response regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Rmet_4965</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="6">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="6">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11827.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519387.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4965"/>
    <dbReference type="EnsemblBacteria" id="ABF11827">
        <property type="protein sequence ID" value="ABF11827"/>
        <property type="gene ID" value="Rmet_4965"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4965"/>
    <dbReference type="eggNOG" id="COG2197">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_90_1_4"/>
    <dbReference type="OMA" id="TITMIDN"/>
    <dbReference type="OrthoDB" id="1687028at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06170">
        <property type="entry name" value="LuxR_C_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016032">
        <property type="entry name" value="Sig_transdc_resp-reg_C-effctor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000792">
        <property type="entry name" value="Tscrpt_reg_LuxR_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00196">
        <property type="entry name" value="GerE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00038">
        <property type="entry name" value="HTHLUXR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00421">
        <property type="entry name" value="HTH_LUXR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46894">
        <property type="entry name" value="SSF46894"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50043">
        <property type="entry name" value="HTH_LUXR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="5" id="KW-0614">Plasmid</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Response regulatory" evidence="4">
        <location>
            <begin position="3"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="domain" description="HTH luxR-type" evidence="3">
        <location>
            <begin position="143"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="2">
        <location>
            <position position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABF11827.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="214" mass="22914" checksum="31FA6CD984379F57" modified="2006-05-30" version="1">MIQVLIADDHPVVVNGIRRELGDEVGFEVVGTAHSSTELLRLLDTTQCDVLVTDYSMPGSATGDGLPMLQLIRRRHPAVRIVVITMLDNPALLRNIQKAGISVILSKSDHLGHLAPAIRAAFVGIAYLAPAVQALLGDASDDGGAPEPKLSKRELEVVRKCAAGVPLVEIARQANRSSKTISAQKSMAMRKLGLTSDYELYQYAVTHGMLAAAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="112" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LDI2</accession>
    <name>Q1LDI2_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Ferredoxin, cytochrome P450</fullName>
            <ecNumber evidence="9">1.14.-.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">thcB</name>
        <name evidence="9" type="ordered locus">Rmet_4932</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="10">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="10">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the cytochrome P450 family.</text>
    </comment>
    <dbReference type="EC" id="1.14.-.-" evidence="9"/>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11794.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519354.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="SMR" id="Q1LDI2"/>
    <dbReference type="STRING" id="266264.Rmet_4932"/>
    <dbReference type="EnsemblBacteria" id="ABF11794">
        <property type="protein sequence ID" value="ABF11794"/>
        <property type="gene ID" value="Rmet_4932"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4932"/>
    <dbReference type="eggNOG" id="COG1018">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG2124">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020412_0_0_4"/>
    <dbReference type="OMA" id="EDEPAHM"/>
    <dbReference type="OrthoDB" id="1885467at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
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        <property type="project" value="InterPro"/>
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    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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    <dbReference type="CDD" id="cd00207">
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        <property type="match status" value="1"/>
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    </dbReference>
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        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="2Fe-2S_ferredoxin-like_sf"/>
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        <property type="entry name" value="2Fe-2S_ferredoxin-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006058">
        <property type="entry name" value="2Fe2S_fd_BS"/>
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        <property type="entry name" value="Beta-grasp_dom_sf"/>
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        <property type="entry name" value="Cyt_P450"/>
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        <property type="entry name" value="Cyt_P450_sf"/>
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        <property type="entry name" value="FAD-bd_FR_type"/>
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        <property type="entry name" value="FNR_nucleotide-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001433">
        <property type="entry name" value="OxRdtase_FAD/NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017938">
        <property type="entry name" value="Riboflavin_synthase-like_b-brl"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00111">
        <property type="entry name" value="Fer2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00175">
        <property type="entry name" value="NAD_binding_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00359">
        <property type="entry name" value="BP450"/>
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    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="SSF48264"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52343">
        <property type="entry name" value="SSF52343"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54292">
        <property type="entry name" value="SSF54292"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63380">
        <property type="entry name" value="SSF63380"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00197">
        <property type="entry name" value="2FE2S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51085">
        <property type="entry name" value="2FE2S_FER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51384">
        <property type="entry name" value="FAD_FR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0001">2Fe-2S</keyword>
    <keyword evidence="2" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="2" id="KW-0288">FMN</keyword>
    <keyword evidence="5" id="KW-0408">Iron</keyword>
    <keyword evidence="6" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-0614">Plasmid</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD-binding FR-type" evidence="8">
        <location>
            <begin position="463"/>
            <end position="566"/>
        </location>
    </feature>
    <feature type="domain" description="2Fe-2S ferredoxin-type" evidence="7">
        <location>
            <begin position="692"/>
            <end position="780"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ABF11794.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="780" mass="86356" checksum="D33B849874EAC55A" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="99" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LDI8</accession>
    <name>Q1LDI8_CUPMC</name>
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        <submittedName>
            <fullName evidence="8">Aromatic amino acid transport protein (APC family)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">aroP</name>
        <name evidence="8" type="ordered locus">Rmet_4926</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="9">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="9">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11788.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519348.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4926"/>
    <dbReference type="EnsemblBacteria" id="ABF11788">
        <property type="protein sequence ID" value="ABF11788"/>
        <property type="gene ID" value="Rmet_4926"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4926"/>
    <dbReference type="eggNOG" id="COG1113">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007946_9_3_4"/>
    <dbReference type="OMA" id="VIMYLTP"/>
    <dbReference type="OrthoDB" id="430372at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006865">
        <property type="term" value="P:amino acid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004841">
        <property type="entry name" value="AA-permease/SLC12A_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004840">
        <property type="entry name" value="Amoino_acid_permease_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00324">
        <property type="entry name" value="AA_permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00218">
        <property type="entry name" value="AMINO_ACID_PERMEASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0614">Plasmid</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="21"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="46"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="99"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="127"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="157"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="199"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="240"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="273"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="331"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="360"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="403"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="427"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="domain" description="AA_permease" evidence="7">
        <location>
            <begin position="18"/>
            <end position="448"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ABF11788.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="462" mass="49655" checksum="9D265F1F51FEC4B5" modified="2006-05-30" version="1">MHAPDNQGTLKRGLKNRHIQLIALGGAIGTGLFLGIAQTIKLAGPSVLLGYALAGVVAFFIMRQLGEMVVDEPVAGSFSYFANKYCGHFAGFMSGWNYWVLYILVSMAELSAVGIYVQYWWPGIPTWISALAFFLIINAINLASVKSFGEMEFWFSIIKVAAIVGMIGFGGYLLATGTAGPDASIANLWQHGGFFPNGFSGLVMAMAVIMFSFGGLELVGITAAEADSPEKSIPKATNQVIYRILIFYVGALAVLLSLYPWEKVVTGGSPFVLIFHAMNSNVVATVLNIVVLTAALSVYNSGVYCNSRMLLGLAQQGNAPRSLMRVNRRGIPLTALAVSAIATGICVVINYFMPGEAFELLMGLVVSALIINWAMISIIHLMFRRAKAAAGKTTRFQSLGYPLTNYVTLAFLAGILVVMFLTPGLRISVYLIPVWLGVLGVSYRLRQKKAGSAMPAASAQIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="103" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LDR0</accession>
    <name>Q1LDR0_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Putative two component transcriptional regulator, LuxR family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Rmet_4854</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="6">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="6">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11716.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519279.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4854"/>
    <dbReference type="EnsemblBacteria" id="ABF11716">
        <property type="protein sequence ID" value="ABF11716"/>
        <property type="gene ID" value="Rmet_4854"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4854"/>
    <dbReference type="eggNOG" id="COG2197">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_90_8_4"/>
    <dbReference type="OMA" id="VAMDVRM"/>
    <dbReference type="OrthoDB" id="1687028at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06170">
        <property type="entry name" value="LuxR_C_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016032">
        <property type="entry name" value="Sig_transdc_resp-reg_C-effctor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000792">
        <property type="entry name" value="Tscrpt_reg_LuxR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00196">
        <property type="entry name" value="GerE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00038">
        <property type="entry name" value="HTHLUXR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00421">
        <property type="entry name" value="HTH_LUXR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46894">
        <property type="entry name" value="SSF46894"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50043">
        <property type="entry name" value="HTH_LUXR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="5" id="KW-0614">Plasmid</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Response regulatory" evidence="4">
        <location>
            <begin position="5"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="domain" description="HTH luxR-type" evidence="3">
        <location>
            <begin position="140"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="2">
        <location>
            <position position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABF11716.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="211" mass="22946" checksum="C65D74C5FAD5259B" modified="2006-05-30" version="1">MSQARLLLIDDHTLFRTGLKLLLAASPNVASIMEAGSVMEATEAHGDKPVDILLLDIQMPGLNGIEGIKVLRRHFSRAPIVIVSGTSGIDAIPDEARKEVAGFLPKSADPREIEEAIACCLAGGTYFQTEQPGDRLPHTRAYKPSQLTPRQLEVLNQLNLGRSNKVIAYHLGLSENTVRVHVAAILDHLGVVSRVEAILEAQRRGLVQAQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LDZ4</accession>
    <name>Q1LDZ4_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative enzyme</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_4770</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the DSD1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11632.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519202.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4770"/>
    <dbReference type="EnsemblBacteria" id="ABF11632">
        <property type="protein sequence ID" value="ABF11632"/>
        <property type="gene ID" value="Rmet_4770"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4770"/>
    <dbReference type="eggNOG" id="COG3616">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031639_3_0_4"/>
    <dbReference type="OMA" id="YVSHDDG"/>
    <dbReference type="OrthoDB" id="1512472at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.37.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001608">
        <property type="entry name" value="Ala_racemase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026956">
        <property type="entry name" value="D-ser_dehydrat-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042208">
        <property type="entry name" value="D-ser_dehydrat-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029066">
        <property type="entry name" value="PLP-binding_barrel"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01168">
        <property type="entry name" value="Ala_racemase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14031">
        <property type="entry name" value="D-ser_dehydrat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01119">
        <property type="entry name" value="D-ser_dehydrat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51419">
        <property type="entry name" value="SSF51419"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="D-ser_dehydrat" evidence="2">
        <location>
            <begin position="311"/>
            <end position="410"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM01119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF11632.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="423" mass="46263" checksum="FCF788AF79DD453D" modified="2006-05-30" version="1">MRETKYQTGMIDPLNKAMGRLDAPLTPSEAGQPGWRLLAEELSLPAAVLYEDRLTHNLQWMRNFMGAYGVQLAPHGKTTMAPKLFARQLAEGAWGITLATAHQTAAAAAHGVKRVLMANQLVGRRNMEIVADLLAADPSFEFFSLVDSAALVDQLGAFFDARGQTLQVLLELGVEGGRTGVRDAAQQADVLDALSRWPNALKLAGVEIYEGVLQQEADIRELLQRTVAVTRELAAAGRFGRSPVVMSGAGSAWYDVVAEEFARTDIGMPIDIVLRPGCYLTHDVGVYRAAQERILASNPVAQKMREGLLPALQLWAYVQSIPEPNRAIIGMGKRDAAFDAGMPIPAQMYRPGAAGPVATPARWKVTGMMDQHAYLEIEAGDDIRVGDMIAFDISHPCLTFDKWRHIPVLDSQFQVIDIVQTFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LE62</accession>
    <name>Q1LE62_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Multicopper oxidase, type 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Rmet_4702</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="5">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="5">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Periplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11564.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519135.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4702"/>
    <dbReference type="EnsemblBacteria" id="ABF11564">
        <property type="protein sequence ID" value="ABF11564"/>
        <property type="gene ID" value="Rmet_4702"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4702"/>
    <dbReference type="eggNOG" id="COG2132">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_543877_0_0_4"/>
    <dbReference type="OMA" id="CHITPPE"/>
    <dbReference type="OrthoDB" id="971126at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042597">
        <property type="term" value="C:periplasmic space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.420">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045087">
        <property type="entry name" value="Cu-oxidase_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011707">
        <property type="entry name" value="Cu-oxidase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008972">
        <property type="entry name" value="Cupredoxin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11709">
        <property type="entry name" value="PTHR11709"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07732">
        <property type="entry name" value="Cu-oxidase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49503">
        <property type="entry name" value="SSF49503"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0614">Plasmid</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193371" evidence="2">
        <location>
            <begin position="26"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="domain" description="Plastocyanin-like" evidence="3">
        <location>
            <begin position="141"/>
            <end position="249"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF07732"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF11564.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="504" mass="54416" checksum="A3D1F632A63BB807" modified="2006-05-30" version="1">MSSSRILRSAFTALLLTSIALPALAQSFRVQCPDSTTLHPAPAQGGGVAAGIKCQQISGGDGYATMGDGTQTYLFSFGPLSGLADIKKGLPGTQPNSVFNTVGSPQTDPTYNGAVGLVPDPEATDANHPLTGHVDPKQIMDVGVMNGNMPAPTMAIDEDDEFFLTLTNVGMIMRPDLFEQHTVHFHGYPNASSFYDGVPDASVAINIGGSFTYYYLAPDAGTYFWHCHITPPEHLQMGMVGQIYVRPRQNRVPMDGTTTLYAALMSQQADPRTRCGTDILCATPLPPQNTFKRVPNKSGTPTLYAYNDGDGSTAYDVEYPIQIHGFDPNFHFVGMTFNPEPFTDMKDKYFLLNGRSYPDTINQAAMHTQVADGTEHFSQPLPTLINIPVGGKALLRISDLDVSEFQTLASLGIPMHVIAVNARLLRDMAGNDMTYYTNSITLGGGESIDVILDASDVNRFPVGSTFYLYTPNLDHLSNDAENFGGLMTEVHICNAVDPASKVCS</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="116" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LEL2</accession>
    <name>Q1LEL2_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="21">Excinuclease ABC, A subunit (UvrA family protein)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="21" type="primary">uvrA2</name>
        <name evidence="21" type="ordered locus">Rmet_4549</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="22">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="22" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="22">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="22">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="18">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11414.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519005.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4549"/>
    <dbReference type="EnsemblBacteria" id="ABF11414">
        <property type="protein sequence ID" value="ABF11414"/>
        <property type="gene ID" value="Rmet_4549"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4549"/>
    <dbReference type="eggNOG" id="COG0178">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001370_3_0_4"/>
    <dbReference type="OMA" id="SKHGWCP"/>
    <dbReference type="OrthoDB" id="152379at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009380">
        <property type="term" value="C:excinuclease repair complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
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        <property type="match status" value="4"/>
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        <property type="entry name" value="uvra"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="3"/>
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        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="11 18" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0997">Cell inner membrane</keyword>
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    <keyword evidence="5" id="KW-0677">Repeat</keyword>
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    <keyword evidence="13" id="KW-1278">Translocase</keyword>
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        <location>
            <begin position="205"/>
            <end position="617"/>
        </location>
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    <feature type="domain" description="ABC transporter" evidence="20">
        <location>
            <begin position="635"/>
            <end position="989"/>
        </location>
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    <feature type="domain" description="ABC transporter" evidence="20">
        <location>
            <begin position="1028"/>
            <end position="1550"/>
        </location>
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        <location>
            <begin position="1340"/>
            <end position="1371"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="19">
        <location>
            <begin position="1340"/>
            <end position="1357"/>
        </location>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <begin position="672"/>
            <end position="679"/>
        </location>
        <ligand>
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            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
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        <location>
            <begin position="1061"/>
            <end position="1068"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022519"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
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        </source>
    </evidence>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022881"/>
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    </evidence>
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        <source>
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        <source>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00023236"/>
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        <source>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="ABF11414.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="1959" mass="212902" checksum="3F789625ED86EBB0" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LEL5</accession>
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        <submittedName>
            <fullName evidence="3">Transcriptional regulator HxlR family</fullName>
        </submittedName>
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        <name evidence="3" type="ordered locus">Rmet_4546</name>
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        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
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            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
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        </source>
    </reference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="HTH_HXLR"/>
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    </dbReference>
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    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="20"/>
            <end position="117"/>
        </location>
    </feature>
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            <begin position="154"/>
            <end position="193"/>
        </location>
    </feature>
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        <location>
            <begin position="163"/>
            <end position="177"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF11411.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="193" mass="21728" checksum="D4D98B8CC0C9FCC5" modified="2006-05-30" version="1">MFSPNGPDDMQRKTFRNMQCPIARSLERVGEWWSILILRDAFYGTKRFDEFQRNLDIAPNMLTRRLNGLVEEGLLEKRQYSERPPRYEYVLTDCGRDFRPVLLALLAWGNRNFAPEGPSVQIVDRETGKVVEPMLVDTENGEPLQMGRRHVLRAGPAADESTRARLSSATSHMTTAAPTAPAAPLAQEAGKVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="70" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LEV5</accession>
    <name>Q1LEV5_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Cupin_2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_4456</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
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        <lineage>
            <taxon>Bacteria</taxon>
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            <taxon>Burkholderiales</taxon>
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        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11321.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4456"/>
    <dbReference type="EnsemblBacteria" id="ABF11321">
        <property type="protein sequence ID" value="ABF11321"/>
        <property type="gene ID" value="Rmet_4456"/>
    </dbReference>
    <dbReference type="GeneID" id="60825921"/>
    <dbReference type="KEGG" id="rme:Rmet_4456"/>
    <dbReference type="eggNOG" id="COG0662">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_139560_0_0_4"/>
    <dbReference type="OMA" id="PGIEQVC"/>
    <dbReference type="OrthoDB" id="1928636at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013096">
        <property type="entry name" value="Cupin_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011051">
        <property type="entry name" value="RmlC_Cupin_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07883">
        <property type="entry name" value="Cupin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51182">
        <property type="entry name" value="SSF51182"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cupin_2" evidence="1">
        <location>
            <begin position="47"/>
            <end position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF07883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF11321.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="129" mass="14127" checksum="2AC56670ACFB02C1" modified="2006-05-30" version="1">MTASPVYFVRTEDVAGYHPANHVGTLNRRIIGRENVGATQLEVIHGTIERGKGALPHAHPGIEQVCYVLEGRARAEVNGHAQELGPGDCCFFPADAMHVFTVISDEPVKLLVVYAPPYEESPDRVIRPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="97" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LEW6</accession>
    <name>Q1LEW6_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Transcriptional regulator, LysR family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_4445</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the LysR transcriptional regulatory family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11310.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518916.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4445"/>
    <dbReference type="EnsemblBacteria" id="ABF11310">
        <property type="protein sequence ID" value="ABF11310"/>
        <property type="gene ID" value="Rmet_4445"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4445"/>
    <dbReference type="eggNOG" id="COG0583">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039613_6_3_4"/>
    <dbReference type="OMA" id="LLHGHFD"/>
    <dbReference type="OrthoDB" id="990666at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd08415">
        <property type="entry name" value="PBP2_LysR_opines_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005119">
        <property type="entry name" value="LysR_subst-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037424">
        <property type="entry name" value="NocR_PBP2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000847">
        <property type="entry name" value="Tscrpt_reg_HTH_LysR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00126">
        <property type="entry name" value="HTH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03466">
        <property type="entry name" value="LysR_substrate"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50931">
        <property type="entry name" value="HTH_LYSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH lysR-type" evidence="2">
        <location>
            <begin position="5"/>
            <end position="62"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF11310.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="296" mass="31773" checksum="73F9DF209F3056F2" modified="2006-05-30" version="1">MPRAPSFREIEVFRAVMLSGTTTAAAAMLHTTQPSVSRVLAQMQTGTDLKLFDMEKGRLRPTPEAQRLFETVQGHFQGLARIGQEVHLLRRSGTGLLRLGCTPTLGLGPMPGIVARFIERHPDVSVDLQTVGSHQLGEGLLHGRFDLVLATGRLDHPQFDIRVMHRSPAVCVMHPGHVMASRRRIRAADLSGQRLLTLNADDELSVALWQALQEAGAEPAATLQTTYSSTICRLAAEGAGIGIVNPYAARVFGQALTVVPLVPAIAVEVRLAHAGHLASSRLTEVFASLVAEGLGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LF07</accession>
    <name>Q1LF07_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Transcriptional regulator, TetR family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">shcR</name>
        <name evidence="3" type="ordered locus">Rmet_4404</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11269.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4404"/>
    <dbReference type="EnsemblBacteria" id="ABF11269">
        <property type="protein sequence ID" value="ABF11269"/>
        <property type="gene ID" value="Rmet_4404"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4404"/>
    <dbReference type="eggNOG" id="COG1309">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_091687_0_0_4"/>
    <dbReference type="OMA" id="HFANKQG"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001647">
        <property type="entry name" value="HTH_TetR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036271">
        <property type="entry name" value="Tet_transcr_reg_TetR-rel_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041479">
        <property type="entry name" value="TetR_CgmR_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17937">
        <property type="entry name" value="TetR_C_28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00440">
        <property type="entry name" value="TetR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00455">
        <property type="entry name" value="HTHTETR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48498">
        <property type="entry name" value="SSF48498"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50977">
        <property type="entry name" value="HTH_TETR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH tetR-type" evidence="2">
        <location>
            <begin position="18"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="H-T-H motif" evidence="1">
        <location>
            <begin position="41"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF11269.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="199" mass="21853" checksum="9F326372ABF8442A" modified="2006-05-30" version="1">MVSSGSMSEAHRRPKQPLLIRDRLLSATIDLLVEEGIGAVTLNSVATRAGVSKGGLQHHFSSKEELLEALSAKVLQHFTGQVATLAESDTDAQGRNTRAYLRASADGFAESGERELWKAAMMLIMARPEFRTPYQEWESGSLNTDLAQCEDPIGLLLCRLAADGLWLADLLGHRAIPPAQRAELVRRLEQLTHCRPQQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-10-07" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LFA6</accession>
    <name>Q1LFA6_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_4304</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11170.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF11170">
        <property type="protein sequence ID" value="ABF11170"/>
        <property type="gene ID" value="Rmet_4304"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4304"/>
    <dbReference type="HOGENOM" id="CLU_2384049_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF11170.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="94" mass="10093" checksum="3949E7891BACB56E" modified="2006-05-30" version="1">MICLSCDCGFENISLPLTVLRAGISSCMTSQCSTTFSVRNTKYVNSQYRLWRPASIATVGHYVVAFSDHHTNLVSEVGGKTSRASLGCLPDSSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LFG7</accession>
    <name>Q1LFG7_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Extra-cytoplasmic solute receptor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">bug</name>
        <name evidence="4" type="ordered locus">Rmet_4242</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="5">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="5">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Periplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the UPF0065 (bug) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11109.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518732.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4242"/>
    <dbReference type="EnsemblBacteria" id="ABF11109">
        <property type="protein sequence ID" value="ABF11109"/>
        <property type="gene ID" value="Rmet_4242"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4242"/>
    <dbReference type="eggNOG" id="COG3181">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045683_0_0_4"/>
    <dbReference type="OMA" id="WKEWIRI"/>
    <dbReference type="OrthoDB" id="447734at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042597">
        <property type="term" value="C:periplasmic space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005064">
        <property type="entry name" value="BUG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042100">
        <property type="entry name" value="Bug_dom1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006311">
        <property type="entry name" value="TAT_signal"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42928">
        <property type="entry name" value="PTHR42928"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03401">
        <property type="entry name" value="TctC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017082">
        <property type="entry name" value="YflP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51318">
        <property type="entry name" value="TAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-0675">Receptor</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193448" evidence="3">
        <location>
            <begin position="30"/>
            <end position="330"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF11109.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="330" mass="35118" checksum="FBD827A5CFE85CD5" modified="2006-05-30" version="1">MNKTRRSTLRLALAATAMATGMMSGTATAQANAWPNRPIRLIVPSAAGGAADFVARTFSAFLQKRLPANAAVVVENRPGAGGIIGTEAAKSAPADGYTFLIASNSTHAANVSLYRNLPYDPIKDFAPVGMFGTFGTILVVPKNSPFKSLPELIRYAKAHPGNMTYGYYSSSSRVPPELLRVRAGLDYAGAPYRNITQIITDMLGGQIQFAFLDSLSAAPALQNPMLAPIAVTSSQRMPQLASVPTVAESLQGFEVQGWLGLTAPAGTPEDVVKRMSTLVSDAISEPAIRTALERQGMAPRTMTPEALRAHMVADRSRWAEWTKAAGIEPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LFM3</accession>
    <name>Q1LFM3_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative hydrolase of the alpha/beta superfamily</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_4186</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11053.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518675.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4186"/>
    <dbReference type="EnsemblBacteria" id="ABF11053">
        <property type="protein sequence ID" value="ABF11053"/>
        <property type="gene ID" value="Rmet_4186"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4186"/>
    <dbReference type="eggNOG" id="COG1073">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029375_2_1_4"/>
    <dbReference type="OMA" id="RDAGHIM"/>
    <dbReference type="OrthoDB" id="721431at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022742">
        <property type="entry name" value="Hydrolase_4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12146">
        <property type="entry name" value="Hydrolase_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="SSF53474"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="16"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="domain" description="Hydrolase_4" evidence="2">
        <location>
            <begin position="88"/>
            <end position="217"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF11053.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="289" mass="31115" checksum="D05B70EA0A912E38" modified="2006-05-30" version="1">MPPPKASPAPNSYRRWLVGAAGLAGVALLAGVGRLILFRAIEREAFVPAPGGTARPDDFGVASRQFAFASGNRMLRGSYVAASPDAPALSVFHGDEECLADWAPVQALLHAAGISSFVFDYSGYGASTGRPSVRHLHQDALAAYAQFRAVTPKASRHYVMAHSLGSGVLLDVVGDLAPAPDGMVIGAGFRSARAAAVVTGRVPRWLAWMLPDPWNNIRQIRNLTMPILLLHSKRDETIPFRDAECLARAAHGPRRLEAFEDLPHDAAIEPSYMTRFWAPVIAYLKSGKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LFN9</accession>
    <name>Q1LFN9_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">NAD-dependent epimerase/dehydratase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_4170</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11037.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4170"/>
    <dbReference type="EnsemblBacteria" id="ABF11037">
        <property type="protein sequence ID" value="ABF11037"/>
        <property type="gene ID" value="Rmet_4170"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4170"/>
    <dbReference type="eggNOG" id="COG0451">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007383_6_0_4"/>
    <dbReference type="OMA" id="GAYKRSK"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001509">
        <property type="entry name" value="Epimerase_deHydtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017829">
        <property type="entry name" value="Hopanoid-assoc_sugar_epimerase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01370">
        <property type="entry name" value="Epimerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03466">
        <property type="entry name" value="HpnA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Epimerase" evidence="1">
        <location>
            <begin position="33"/>
            <end position="259"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF11037.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="358" mass="38866" checksum="23668FAE95A685F9" modified="2006-05-30" version="1">MLHRNPAVGGLLRGRRACRAGSIVRGFEINNCILVTGASGFLGSSVMRQALDRGFQVRVLVRSTSPRANLAGLPVEIVEGDMRDAASMTRAMAGVRYLFHVAADYRLWAPDPEEIVRSNVEGTVTVMNAARAAGVERVIYTSSVATLRVAGATAPVDETAAMAGHEAIGAYKRSKVLAEREVERLVAEGLPAVIVNPSTPIGPRDVRPTPTGRIIVEAATGKIPAFVDTGLNLVHVDDVANGHFLALDKGRIGERYILGGQDVLLRQMLADIADMAGRKAPTIELPRWPLYPVARVAETIARFTGKEPFVTVDGLKMSQYRMFFTSAKAQRELGYAPRPYQEGLRDALTWFREAGYLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-10-07" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LFV0</accession>
    <name>Q1LFV0_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Extra-cytoplasmic solute receptor protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">bug</name>
        <name evidence="4" type="ordered locus">Rmet_4109</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="5">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="5">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Periplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the UPF0065 (bug) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10976.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4109"/>
    <dbReference type="EnsemblBacteria" id="ABF10976">
        <property type="protein sequence ID" value="ABF10976"/>
        <property type="gene ID" value="Rmet_4109"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4109"/>
    <dbReference type="eggNOG" id="COG3181">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045683_0_2_4"/>
    <dbReference type="OMA" id="LAVNPHT"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042597">
        <property type="term" value="C:periplasmic space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005064">
        <property type="entry name" value="BUG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042100">
        <property type="entry name" value="Bug_dom1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006311">
        <property type="entry name" value="TAT_signal"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42928">
        <property type="entry name" value="PTHR42928"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03401">
        <property type="entry name" value="TctC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017082">
        <property type="entry name" value="YflP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51318">
        <property type="entry name" value="TAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-0675">Receptor</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193453" evidence="3">
        <location>
            <begin position="38"/>
            <end position="334"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF10976.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="334" mass="34699" checksum="99513D0794EA7C61" modified="2006-05-30" version="1">MQVSPTRPTRPHRRRSLSSMASLAAATLLSIPFTAHATGYPDKPITLVVPFGAGGITDLIARATGKALSEQLGQSVVVENRPGAGGNIAADFVRRARPDGYTLMFATVGVLAVNPHTDVKVNFDSAKDFTYISLVGSTPFLVVVGADVPANTLPALIKLAQRKPDAISVGTAGVGSAPYQGMRIFQDVAKVEFLHVPFKSGAESVTNVVSGQVNMTFEATPQVMPFVTSGKLRALAVASPHRLSTAPQVPSTAELGFPSLVSGSVAGLIGPAGLDPAIVKKLNAAVAKVAADPKFKATLIAQGTEPAASSPEQFRKLISDENQRWSALLGAGNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LFW0</accession>
    <name>Q1LFW0_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Acyl-CoA transferase/L-carnitine dehydratase, CaiB</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">caiB</name>
        <name evidence="1" type="ordered locus">Rmet_4099</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10966.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4099"/>
    <dbReference type="EnsemblBacteria" id="ABF10966">
        <property type="protein sequence ID" value="ABF10966"/>
        <property type="gene ID" value="Rmet_4099"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4099"/>
    <dbReference type="eggNOG" id="COG1804">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033975_2_1_4"/>
    <dbReference type="OrthoDB" id="969868at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1540.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10540">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003673">
        <property type="entry name" value="CoA-Trfase_fam_III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044855">
        <property type="entry name" value="CoA-Trfase_III_dom3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023606">
        <property type="entry name" value="CoA-Trfase_III_dom_1_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02515">
        <property type="entry name" value="CoA_transf_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89796">
        <property type="entry name" value="SSF89796"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF10966.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="411" mass="43577" checksum="CA5CB6B02FF12D5D" modified="2006-05-30" version="1">MQKMEANKFRPLAGVKVLELSQILAGPTCGLMLGDLGADVVKIERFPAGDDARKYRRQGDSGLPPSFLIVNRGKRSLGLDLRKEEGKAVLKRMVAQADVLTENFRLGVMEKLGLGYDVLREVNPALIYCSITGYGRTGPLASKGGFDLILQAFSGLISVTGEPGRDPVKPGMSIADVNAGILAAFGILAAYINRLRTGEGSRVETSLLQASMQQLYWYAAAYFSTGAIAKPVGTAHPLIAPYQVYQCADGGIALGGANQTNWERIADVLGHPEWKADPRFDSAAERLARRAELELLITDVLKGAPVATWVERFDAVGVPVGPVQNVAQALEHPQAKAVGMVVEARKPGGGMTRALGLPVLFDGGNASSTGPAPALGEHSSSVLREFGFAAGEIEDLLRNAVVHDCAHPVAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-12-02" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LGM0</accession>
    <name>Q1LGM0_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Heavy metal cation tricomponent efflux outer membrane porin HmvC (CzcC-like)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">hmvC</name>
        <name evidence="4" type="ordered locus">Rmet_3836</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="5">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="5">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the outer membrane factor (OMF) (TC 1.B.17) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10706.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3836"/>
    <dbReference type="EnsemblBacteria" id="ABF10706">
        <property type="protein sequence ID" value="ABF10706"/>
        <property type="gene ID" value="Rmet_3836"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3836"/>
    <dbReference type="eggNOG" id="COG1538">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012817_14_4_4"/>
    <dbReference type="OMA" id="DALRCNR"/>
    <dbReference type="OrthoDB" id="1533572at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015562">
        <property type="term" value="F:efflux transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003423">
        <property type="entry name" value="OMP_efflux"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02321">
        <property type="entry name" value="OEP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0614">Plasmid</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="333"/>
            <end position="367"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF10706.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="445" mass="46974" checksum="A7823178F7142865" modified="2006-05-30" version="1">MASVGSIAASAGQAASGPMPPDGPCMPPPAKAALTLAQARLDALRCNRDIISARRGEEASQADIQIASQRPNPVLSLGVANINPHLGVGAGNWRSKTVDSTVRVDQVIETANKGNLRTDAARKASAAAGEQTQAVVAQQVGAVEQAWYDAVASQSRVEVLTETVGLYGKTQQANEARLKAGDVARADVTKLQLETLRAQSDLRSAQSDYATSKAALAATIGLPGTLLDNRLVPDWPALDAPVPSVDPEVLQRRPDVTAAEARLAAAASARDLARAGRVPDVTVGAQYEHYPVSEANTTGSGNSYGVFISIPLFVRHSFGGEARRAEVDYYAALDDRNRILLAAQNDIERLRSQLESARASLQQMQTAVLPAAETVAQSAEFAYTKGATGVLDLLDARRALRQTRLDAVDVQGNFAKAMAAYRAALQATTSDINKLPQVRTDASHP</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-12-02" version="72" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LGV9</accession>
    <name>Q1LGV9_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative GGDEF/cyclase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_3745</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10617.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3745"/>
    <dbReference type="EnsemblBacteria" id="ABF10617">
        <property type="protein sequence ID" value="ABF10617"/>
        <property type="gene ID" value="Rmet_3745"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3745"/>
    <dbReference type="eggNOG" id="COG2199">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_11_36_4"/>
    <dbReference type="OMA" id="ICEPYDL"/>
    <dbReference type="OrthoDB" id="1635706at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01949">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000160">
        <property type="entry name" value="GGDEF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029787">
        <property type="entry name" value="Nucleotide_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00990">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00267">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55073">
        <property type="entry name" value="SSF55073"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00254">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50887">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="45"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="76"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="108"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="136"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="164"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="domain" description="GGDEF" evidence="2">
        <location>
            <begin position="255"/>
            <end position="388"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF10617.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="392" mass="42531" checksum="DDAA652C3B59D170" modified="2006-05-30" version="1">MSPTHKPMPEPTEVFTLPRWKITRWLTDTDRDVPTDIKRALIGSLFGTLSIFVGGVVNTLLVAALIAWHIQRPAFFAWFGLECLICIARISILVSARRRARAARSTHTDLHLALSLAWAFSVGLGTYLSITSGNWLAAALACLSSAAMIGGICFRNFGAPRMSGLMILLALGPCCLGAIVAREPLFLLTLIQLPVYVASMTSASFRMHAMLVATMQSERENAHRARHDSMTGLLNRGSLLAEIRATMECNAISPLRSALLYLDLDGFKRINDVHGHGCGDELLQQVADRMRATVPEGTHVSRIGGDEFVILLNLHESDDVQVLAQRLLRDIAVPYLLANGETVAIGVSIGITHISPNGQSADAVLNVADRALYVAKARGKGCFHFSEAVQAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LGX3</accession>
    <name>Q1LGX3_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Flagella synthesis protein FlgN</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">flgN</name>
        <name evidence="3" type="ordered locus">Rmet_3731</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Required for the efficient initiation of filament assembly.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the FlgN family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10603.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008641151.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3731"/>
    <dbReference type="EnsemblBacteria" id="ABF10603">
        <property type="protein sequence ID" value="ABF10603"/>
        <property type="gene ID" value="Rmet_3731"/>
    </dbReference>
    <dbReference type="GeneID" id="60826285"/>
    <dbReference type="KEGG" id="rme:Rmet_3731"/>
    <dbReference type="HOGENOM" id="CLU_137423_1_1_4"/>
    <dbReference type="OrthoDB" id="1696769at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044780">
        <property type="term" value="P:bacterial-type flagellum assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007809">
        <property type="entry name" value="FlgN-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036679">
        <property type="entry name" value="FlgN-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05130">
        <property type="entry name" value="FlgN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140566">
        <property type="entry name" value="SSF140566"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0966">Cell projection</keyword>
    <keyword evidence="3" id="KW-0969">Cilium</keyword>
    <keyword evidence="3" id="KW-0282">Flagellum</keyword>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002397"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF10603.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="154" mass="16050" checksum="DCAB207AEDE305BA" modified="2006-05-30" version="1">MTQAALIQTLSIEAGAIAAFGRALADEREAIKRQDFQALTDLLGKKMELAQALTRATAAREAQMIALKLRVGAGGLLIGGPLEPGVAEAWRKVVFFAHKARDANELNGAIVNAHLEFTQEAIQVLRQGGAGPNEMYGRNGKASATAGGVSLAAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="117" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LH15</accession>
    <name>Q1LH15_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Chemotaxis protein CheA</fullName>
            <ecNumber evidence="2">2.7.13.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">cheA</name>
        <name evidence="12" type="ordered locus">Rmet_3689</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="13">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
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            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="13">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="13">Plasmid megaplasmid CH34</plasmid>
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    </dbReference>
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        <location>
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            <end position="527"/>
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        <location>
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            <end position="664"/>
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            <dbReference type="PROSITE" id="PS50894"/>
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    </evidence>
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            <dbReference type="EMBL" id="ABF10561.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="671" mass="71680" checksum="E8583680D583F47F" modified="2006-05-30" version="1"> </sequence>
</entry>
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        </submittedName>
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        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
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        </source>
    </evidence>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
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        </source>
    </evidence>
    <sequence length="426" mass="45452" checksum="1255BF5F523BBA8B" modified="2006-05-30" version="1">MQRRSVLQAALAGVAATTLGMLTPRQARAATAAKVVVVGGGFGGATAARYLRTWSHGAVDVTLIEPDEAFVSCPLSNLVVAGYRQMADITLPYDTLVSRHGVKHVRDTVTAIDPAARTVRVAGGGTLPYDRLILSPGVEMQSSAIPGLARPGGDQIVHAWKAGPQTDVLRRQLAAMPDGGTFAITIPLAPYRCPPGPYERACLVADYLKQHKPRSKVLVLDANPDITSKGALFRKVWETRYKGMIEYRPQFETVDVDPATRTIRFDVQDDERADVINLLPPQRAGAIAVASGLATANGRWCEVDFLTMASTVAPDIHVLGDAVQIAPLMPKSGTMANQHGKVAAAAILRLLAGQDPDPEPVYTNTCYSFTSSNEAIHVASVHRYDAGEKTMLTVPGSGGLSVAPSRREGEYGLAWAHSIWADMLGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="88" xmlns="http://uniprot.org/uniprot">
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        <submittedName>
            <fullName evidence="10">Cytochrome c1 (Transmembrane protein)</fullName>
        </submittedName>
    </protein>
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        <name evidence="10" type="primary">petC</name>
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    </gene>
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        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
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                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
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    </dbReference>
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    </dbReference>
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        <property type="gene ID" value="Rmet_3228"/>
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        <property type="taxonomic scope" value="Bacteria"/>
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    <dbReference type="HOGENOM" id="CLU_078597_0_0_4"/>
    <dbReference type="OMA" id="MPHVLWE"/>
    <dbReference type="OrthoDB" id="1426747at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
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        <property type="term" value="C:integral component of membrane"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="F:electron transfer activity"/>
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        <property type="project" value="InterPro"/>
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        <property type="term" value="F:heme binding"/>
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        <property type="project" value="InterPro"/>
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        <property type="entry name" value="Cyt_c-like_dom"/>
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        <property type="entry name" value="Cyt_c-like_dom_sf"/>
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    <dbReference type="InterPro" id="IPR002326">
        <property type="entry name" value="Cyt_c1"/>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02167">
        <property type="entry name" value="Cytochrom_C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46626">
        <property type="entry name" value="SSF46626"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51007">
        <property type="entry name" value="CYTC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 6" id="KW-0349">Heme</keyword>
    <keyword evidence="5 6" id="KW-0408">Iron</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <keyword evidence="2 7 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193188" evidence="8">
        <location>
            <begin position="23"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="220"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="domain" description="Cytochrome c" evidence="9">
        <location>
            <begin position="40"/>
            <end position="210"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00433"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ABF10100.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="247" mass="27822" checksum="558FC7D5DE5F30E8" modified="2006-05-30" version="1">MKKLLSIFALVGACFAAMPAMASEGGFPLEPAPLDTSDVSSLQRGAKLFVNYCLNCHGASMMRYNRLKGLDLTDEQIQQNLLFSAGKVGETMTIAMHPKDAKAFFGAIPPDLSVIARARGNDWLYTYLRTFYRDDTRATGWNNLVFPSVGMPHVLWELQGQRAAKFAEAEEHGEKVHKFTGFEQITPGKLSSQEYDQAAADLVNYLNWMAEPEQSHRKRLGVWVLLFLGMFTVFAWRLNAAYWKDVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LIU8</accession>
    <name>Q1LIU8_CUPMC</name>
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        <submittedName>
            <fullName evidence="3">Exoribonuclease II (RNB)-family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_3056</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
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        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
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    </dbReference>
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        <property type="gene ID" value="Rmet_3056"/>
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        <property type="component" value="Chromosome"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
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        <property type="entry name" value="RNase_II/R"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00773">
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        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00955">
        <property type="entry name" value="RNB"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SSF50249"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
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            <begin position="235"/>
            <end position="525"/>
        </location>
    </feature>
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        <location>
            <begin position="641"/>
            <end position="712"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="641"/>
            <end position="657"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="681"/>
            <end position="696"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00955"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF09928.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="712" mass="77734" checksum="08CEFA56DAD3C40F" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="101" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LJ23</accession>
    <name>Q1LJ23_CUPMC</name>
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        <recommendedName>
            <fullName evidence="4">Transketolase</fullName>
            <ecNumber evidence="4">2.2.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">cbbT2</name>
        <name evidence="10" type="ordered locus">Rmet_2980</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>thiamine diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58937"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the transketolase family.</text>
    </comment>
    <dbReference type="EC" id="2.2.1.1" evidence="4"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09853.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2980"/>
    <dbReference type="EnsemblBacteria" id="ABF09853">
        <property type="protein sequence ID" value="ABF09853"/>
        <property type="gene ID" value="Rmet_2980"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2980"/>
    <dbReference type="eggNOG" id="COG0021">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009227_0_0_4"/>
    <dbReference type="OMA" id="HHTEGIE"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004802">
        <property type="term" value="F:transketolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd02012">
        <property type="entry name" value="TPP_TK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009014">
        <property type="entry name" value="Transketo_C/PFOR_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005475">
        <property type="entry name" value="Transketolase-like_Pyr-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005478">
        <property type="entry name" value="Transketolase_bac-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033248">
        <property type="entry name" value="Transketolase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033247">
        <property type="entry name" value="Transketolase_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005474">
        <property type="entry name" value="Transketolase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43522">
        <property type="entry name" value="PTHR43522"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02779">
        <property type="entry name" value="Transket_pyr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02780">
        <property type="entry name" value="Transketolase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00456">
        <property type="entry name" value="Transketolase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00861">
        <property type="entry name" value="Transket_pyr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="SSF52518"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52922">
        <property type="entry name" value="SSF52922"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00232">
        <property type="entry name" value="tktlase_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00801">
        <property type="entry name" value="TRANSKETOLASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0786">Thiamine pyrophosphate</keyword>
    <keyword evidence="5 10" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="TRANSKETOLASE_1" evidence="9">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007131"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS00801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ABF09853.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="659" mass="71087" checksum="D4BE353273F2522C" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="107" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LJE1</accession>
    <name>Q1LJE1_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Acetylornithine aminotransferase</fullName>
            <shortName evidence="2">ACOAT</shortName>
            <ecNumber evidence="2">2.6.1.11</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2 4" type="primary">argD</name>
        <name evidence="4" type="ordered locus">Rmet_2862</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>2-oxoglutarate + N(2)-acetyl-L-ornithine = L-glutamate + N-acetyl-L-glutamate 5-semialdehyde</text>
            <dbReference type="Rhea" id="RHEA:18049"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:29123"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:57805"/>
            <dbReference type="EC" id="2.6.1.11"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
        <text evidence="2">Binds 1 pyridoxal phosphate per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Amino-acid biosynthesis; L-arginine biosynthesis; N(2)-acetyl-L-ornithine from L-glutamate: step 4/4.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="miscellaneous">
        <text evidence="2">May also have succinyldiaminopimelate aminotransferase activity, thus carrying out the corresponding step in lysine biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family. ArgD subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.6.1.11" evidence="2"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09735.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008644896.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2862"/>
    <dbReference type="EnsemblBacteria" id="ABF09735">
        <property type="protein sequence ID" value="ABF09735"/>
        <property type="gene ID" value="Rmet_2862"/>
    </dbReference>
    <dbReference type="GeneID" id="60820699"/>
    <dbReference type="KEGG" id="rme:Rmet_2862"/>
    <dbReference type="eggNOG" id="COG4992">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016922_10_1_4"/>
    <dbReference type="OMA" id="FWGWQAH"/>
    <dbReference type="OrthoDB" id="572533at2"/>
    <dbReference type="UniPathway" id="UPA00068">
        <property type="reaction ID" value="UER00109"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003992">
        <property type="term" value="F:N2-acetyl-L-ornithine:2-oxoglutarate 5-aminotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006526">
        <property type="term" value="P:arginine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00610">
        <property type="entry name" value="OAT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01107">
        <property type="entry name" value="ArgD_aminotrans_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004636">
        <property type="entry name" value="AcOrn/SuccOrn_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005814">
        <property type="entry name" value="Aminotrans_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00202">
        <property type="entry name" value="Aminotran_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000521">
        <property type="entry name" value="Transaminase_4ab_Lys_Orn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="SSF53383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00600">
        <property type="entry name" value="AA_TRANSFER_CLASS_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="2 4" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="2" id="KW-0055">Arginine biosynthesis</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="1 2 3" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Pyridoxal phosphate binding" evidence="2">
        <location>
            <begin position="102"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="region of interest" description="Pyridoxal phosphate binding" evidence="2">
        <location>
            <begin position="219"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="134"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="137"/>
        </location>
        <ligand>
            <name>N(2)-acetyl-L-ornithine</name>
            <dbReference type="ChEBI" id="CHEBI:57805"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="276"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="2">
        <location>
            <position position="248"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF09735.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="395" mass="42931" checksum="51DFFF9693101429" modified="2006-05-30" version="1">MAFAEFPVQSLMYITNRPELVFTEGKGSWLTDHNGKRYLDFVQGWAVNALGHSNEGMIQALEKQARKLLNPSPAFYNEPMLKLAQQLTDNSCFDKVFFANSGAEANEGAIKLARKWGRKHKNGAYEIITMDHSFHGRTLATMSASGKAGWDTIFAPQVPGFPKAELNDLASVEKLITDKTVGIMLEPVQGEGGVIPATREFMQGLRALADKHKLLFIVDEVQTGCGRCGTLFAYELSGVEPDIMTLGKGIGGGVPLSALLCKADVASFEAGDQGGTYNGNPLMTAVGSSVMEQLTAPGFLDDVKAKGEYLREQLLVLTKEFGLGGERGEGLLRALVLGKDIGPQLVEEAREMSPVGLLLNAPRPNLLRFMPALNVTREEIDQMIGMLRELLKKLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-10-07" version="67" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LJH4</accession>
    <name>Q1LJH4_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">HTH cro/C1-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_2829</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09702.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2829"/>
    <dbReference type="EnsemblBacteria" id="ABF09702">
        <property type="protein sequence ID" value="ABF09702"/>
        <property type="gene ID" value="Rmet_2829"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2829"/>
    <dbReference type="HOGENOM" id="CLU_2002009_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00093">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001387">
        <property type="entry name" value="Cro/C1-type_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010982">
        <property type="entry name" value="Lambda_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13443">
        <property type="entry name" value="HTH_26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47413">
        <property type="entry name" value="SSF47413"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH cro/C1-type" evidence="1">
        <location>
            <begin position="18"/>
            <end position="52"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF09702.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="124" mass="14085" checksum="4B722D1B55090E32" modified="2006-05-30" version="1">MSPKNMSSATPEEMAETIRKAWRRRDISQKQVERDLGIHQSQFSKLVNGRFKEANGHASRLFEYSKRHEGTAQLQSGETDTEALRSALTERLMRAWDGTDEGARALGAILDGVARLRARRSRRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LJU4</accession>
    <name>Q1LJU4_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Protein-L-isoaspartate O-methyltransferase</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">L-isoaspartyl protein carboxyl methyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="3">Protein L-isoaspartyl methyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="4">Protein-beta-aspartate methyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">pcm</name>
        <name evidence="6" type="ordered locus">Rmet_2709</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the methyltransferase superfamily. L-isoaspartyl/D-aspartyl protein methyltransferase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09582.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517281.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2709"/>
    <dbReference type="EnsemblBacteria" id="ABF09582">
        <property type="protein sequence ID" value="ABF09582"/>
        <property type="gene ID" value="Rmet_2709"/>
    </dbReference>
    <dbReference type="GeneID" id="60820852"/>
    <dbReference type="KEGG" id="rme:Rmet_2709"/>
    <dbReference type="eggNOG" id="COG2518">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_055432_2_1_4"/>
    <dbReference type="OMA" id="QIRTWEV"/>
    <dbReference type="OrthoDB" id="1948290at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004719">
        <property type="term" value="F:protein-L-isoaspartate (D-aspartate) O-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000682">
        <property type="entry name" value="PCMT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11579">
        <property type="entry name" value="PTHR11579"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005369"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013346"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00030757"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00031323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00031350"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABF09582.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="217" mass="23662" checksum="9530E96CE38709EA" modified="2006-05-30" version="1">MDLEKARFNMIEQQIRPWDVLDQEILDLLAVVKREQFVPQAYAALAFVDMEIPLPCGENMLPPRVEARLLQDLNVRKHENVLEIGAGSGYMAALLANRARHVLTVDVHPELVALARQNLANAGITNVDVAEGNAAEGWAVAAPYDVICISASVPEIPSAILSQVKVGGRIAAIVGSAPVMEARIITRASETEYQVVDLFETLAKPLEGAVQPSRFKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="100" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LK32</accession>
    <name>Q1LK32_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Exodeoxyribonuclease 7 small subunit</fullName>
            <ecNumber evidence="3">3.1.11.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">Exodeoxyribonuclease VII small subunit</fullName>
            <shortName evidence="3">Exonuclease VII small subunit</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="3 5" type="primary">xseB</name>
        <name evidence="5" type="ordered locus">Rmet_2617</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Bidirectionally degrades single-stranded DNA into large acid-insoluble oligonucleotides, which are then degraded further into small acid-soluble oligonucleotides.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>Exonucleolytic cleavage in either 5'- to 3'- or 3'- to 5'-direction to yield nucleoside 5'-phosphates.</text>
            <dbReference type="EC" id="3.1.11.6"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="3">Heterooligomer composed of large and small subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the XseB family.</text>
    </comment>
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        <property type="protein sequence ID" value="ABF09494.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2617"/>
    <dbReference type="EnsemblBacteria" id="ABF09494">
        <property type="protein sequence ID" value="ABF09494"/>
        <property type="gene ID" value="Rmet_2617"/>
    </dbReference>
    <dbReference type="GeneID" id="60820947"/>
    <dbReference type="KEGG" id="rme:Rmet_2617"/>
    <dbReference type="eggNOG" id="COG1722">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_145918_2_0_4"/>
    <dbReference type="OMA" id="GDMLKPF"/>
    <dbReference type="OrthoDB" id="2057189at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009318">
        <property type="term" value="C:exodeoxyribonuclease VII complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008855">
        <property type="term" value="F:exodeoxyribonuclease VII activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006308">
        <property type="term" value="P:DNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1040">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00337">
        <property type="entry name" value="Exonuc_7_S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003761">
        <property type="entry name" value="Exonuc_VII_S"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037004">
        <property type="entry name" value="Exonuc_VII_ssu_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34137">
        <property type="entry name" value="PTHR34137"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02609">
        <property type="entry name" value="Exonuc_VII_S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF116842">
        <property type="entry name" value="SSF116842"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01280">
        <property type="entry name" value="xseB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 3 5" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="3 5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0540">Nuclease</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="79"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="86"/>
            <end position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009998"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00337"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABF09494.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="102" mass="10865" checksum="38201EFF4722AC55" modified="2006-05-30" version="1">MPRTTTAPVQPEDTPAASVPPASYEAAMAELETLVASMESGDLPLEASLAAYRRGAELVRFCQQSLERVAQQVKVLEGDALKPLAGESRTQNNADSNSADFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="96" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LK66</accession>
    <name>Q1LK66_CUPMC</name>
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        <submittedName>
            <fullName evidence="5">Phosphodiesterase/alkaline phosphatase D</fullName>
            <ecNumber evidence="5">3.1.3.1</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">phoD</name>
        <name evidence="5" type="ordered locus">Rmet_2583</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EC" id="3.1.3.1" evidence="5"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09460.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2583"/>
    <dbReference type="EnsemblBacteria" id="ABF09460">
        <property type="protein sequence ID" value="ABF09460"/>
        <property type="gene ID" value="Rmet_2583"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2583"/>
    <dbReference type="eggNOG" id="COG3540">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_525464_0_0_4"/>
    <dbReference type="OMA" id="DHEVRNN"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004035">
        <property type="term" value="F:alkaline phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd07389">
        <property type="entry name" value="MPP_PhoD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018946">
        <property type="entry name" value="PhoD-like_MPP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038607">
        <property type="entry name" value="PhoD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032093">
        <property type="entry name" value="PhoD_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006311">
        <property type="entry name" value="TAT_signal"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09423">
        <property type="entry name" value="PhoD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16655">
        <property type="entry name" value="PhoD_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="SSF56300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51318">
        <property type="entry name" value="TAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="56"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="domain" description="PhoD_N" evidence="4">
        <location>
            <begin position="87"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="domain" description="PhoD" evidence="3">
        <location>
            <begin position="195"/>
            <end position="549"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="12"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="16"/>
            <end position="39"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF09423"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF16655"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABF09460.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="567" mass="63024" checksum="7245FD3C7F1B6B56" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LKC4</accession>
    <name>Q1LKC4_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">AMP-dependent synthetase and ligase</fullName>
            <ecNumber evidence="4">6.2.1.26</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Rmet_2525</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="ABF09402.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517101.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2525"/>
    <dbReference type="EnsemblBacteria" id="ABF09402">
        <property type="protein sequence ID" value="ABF09402"/>
        <property type="gene ID" value="Rmet_2525"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2525"/>
    <dbReference type="eggNOG" id="COG0318">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000022_59_7_4"/>
    <dbReference type="OMA" id="WLMQRAF"/>
    <dbReference type="OrthoDB" id="377638at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008756">
        <property type="term" value="F:o-succinylbenzoate-CoA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025110">
        <property type="entry name" value="AMP-bd_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045851">
        <property type="entry name" value="AMP-bd_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020845">
        <property type="entry name" value="AMP-binding_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_Synth/Lig"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13193">
        <property type="entry name" value="AMP-binding_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00455">
        <property type="entry name" value="AMP_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0436">Ligase</keyword>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="209"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="domain" description="AMP-binding" evidence="2">
        <location>
            <begin position="8"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="domain" description="AMP-binding_C" evidence="3">
        <location>
            <begin position="429"/>
            <end position="504"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF13193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF09402.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="516" mass="56189" checksum="1AE97DF1401DDBB4" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LKT3</accession>
    <name>Q1LKT3_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Putative enoyl-CoA hydratase/isomerase</fullName>
            <ecNumber evidence="5">4.2.1.17</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Rmet_2366</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Lipid metabolism; fatty acid beta-oxidation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 4">Belongs to the enoyl-CoA hydratase/isomerase family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.17" evidence="5"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09243.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_003149781.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2366"/>
    <dbReference type="EnsemblBacteria" id="ABF09243">
        <property type="protein sequence ID" value="ABF09243"/>
        <property type="gene ID" value="Rmet_2366"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2366"/>
    <dbReference type="eggNOG" id="COG1024">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009834_7_6_4"/>
    <dbReference type="OMA" id="WHRAFDK"/>
    <dbReference type="OrthoDB" id="1498685at2"/>
    <dbReference type="UniPathway" id="UPA00659"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004300">
        <property type="term" value="F:enoyl-CoA hydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016853">
        <property type="term" value="F:isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006635">
        <property type="term" value="P:fatty acid beta-oxidation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.12.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029045">
        <property type="entry name" value="ClpP/crotonase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045002">
        <property type="entry name" value="Ech1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018376">
        <property type="entry name" value="Enoyl-CoA_hyd/isom_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001753">
        <property type="entry name" value="Enoyl-CoA_hydra/iso"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014748">
        <property type="entry name" value="Enoyl-CoA_hydra_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43149">
        <property type="entry name" value="PTHR43149"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00378">
        <property type="entry name" value="ECH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52096">
        <property type="entry name" value="SSF52096"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00166">
        <property type="entry name" value="ENOYL_COA_HYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0413">Isomerase</keyword>
    <keyword evidence="5" id="KW-0456">Lyase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABF09243.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="289" mass="30966" checksum="812B3538142CD393" modified="2006-05-30" version="1">MPAGPSISTCPPQTTMFMTHCKENFMSEPILLTSEQDNIGIVTLNMAHKRNALNEEAIAAIDAYFSAVPSHIRAILLTAKGDHFCAGLDLKEHHDKARSGVEFMRVCQGWHRAFDKIQHGGIPVVACLQGAVVGGGLELASAAHVRVADSRTFFALPEGTRGIFTGGGATVRTARIISAPRMVEMMLTGRVLDAREGRELGLAHYVCDIAKGDTPAPEFALTLAKKIAGNAPLSNYAIVSAISRIADMSATDGLFTEGLVMAMIQQGDDVQERLGDFVNKKAHKVQLNA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LL46</accession>
    <name>Q1LL46_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Glucanase</fullName>
            <ecNumber evidence="4">3.2.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">bcsZ</name>
        <name evidence="6" type="ordered locus">Rmet_2251</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Endohydrolysis of (1-&gt;4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.</text>
            <dbReference type="EC" id="3.2.1.4"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 4">Belongs to the glycosyl hydrolase 8 (cellulase D) family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.-" evidence="4"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09130.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516953.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2251"/>
    <dbReference type="CAZy" id="GH8">
        <property type="family name" value="Glycoside Hydrolase Family 8"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF09130">
        <property type="protein sequence ID" value="ABF09130"/>
        <property type="gene ID" value="Rmet_2251"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2251"/>
    <dbReference type="eggNOG" id="COG3405">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037297_0_0_4"/>
    <dbReference type="OMA" id="IRVYLWV"/>
    <dbReference type="OrthoDB" id="636673at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008810">
        <property type="term" value="F:cellulase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000272">
        <property type="term" value="P:polysaccharide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008928">
        <property type="entry name" value="6-hairpin_glycosidase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012341">
        <property type="entry name" value="6hp_glycosidase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002037">
        <property type="entry name" value="Glyco_hydro_8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019834">
        <property type="entry name" value="Glyco_hydro_8_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01270">
        <property type="entry name" value="Glyco_hydro_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00735">
        <property type="entry name" value="GLHYDRLASE8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48208">
        <property type="entry name" value="SSF48208"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00812">
        <property type="entry name" value="GLYCOSYL_HYDROL_F8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="4 6" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="4 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0624">Polysaccharide degradation</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193186" description="Glucanase" evidence="5">
        <location>
            <begin position="27"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="3">
        <location>
            <position position="126"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10058"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU361167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABF09130.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="387" mass="42410" checksum="1A442F97BAB6D57D" modified="2006-05-30" version="1">MRRAMAWLLRATVVCTLAGTVLTSAAATCAWPDWDAFRRSTISQDGRVIDDSTDQQVTVSEGQSYALFFALVANDRATFDKLLTWTENNLAQGDLTAHLPAWIWGKRTEGEHAGTWGVIDANPASDADLWIAYSLLEAGRLWNERRFTALGTLLARRALREETAVLPGLGRTLLPGPTGFHTSPDVWRLNPSYVPLQVMRRLAAMPGEDSSWKTLVDTSARLILNTAPHGFSPDWVEYHKGRGFRPDTQTQAGGSYNAIRVYLWAGMMAPRDPLRTSVLQAFRPMADYVRKQGDPPERVDTQSGTFGPNSGNGGFSAAVAPYLAALGQTDDARAQAQRARDLAAQKPAGYYSQVLALFGLGHLDGHFRFEADGTLVPAWKSTCPATR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="49" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LLC7</accession>
    <name>Q1LLC7_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_2170</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09049.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516879.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2170"/>
    <dbReference type="EnsemblBacteria" id="ABF09049">
        <property type="protein sequence ID" value="ABF09049"/>
        <property type="gene ID" value="Rmet_2170"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2170"/>
    <dbReference type="HOGENOM" id="CLU_1244485_0_0_4"/>
    <dbReference type="OrthoDB" id="2178650at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="109"/>
            <end position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF09049.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="222" mass="25421" checksum="B6A2D416F455E770" modified="2006-05-30" version="1">MKKPSSFAAWQEANQVRREAIIEDYLQYLGKTRVRVRNPTDLADLVARHIAQVEDGPCNKSTLMRNFRYKAKILTYQAEHSESGSRSLRPRGVTDPSTNALVTIAKLEAGNLKREVGRLNIYINSLEEQLEQYQQQGRQRLSELVAHKAESGAHRISDYEFKFVRTCQALRALVSHMNLVLEVDTKAQRILDRSKRRNNVIVDNEIAGPFIEWLASVSEEVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-10-07" version="51" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LLD7</accession>
    <name>Q1LLD7_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_2160</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09039.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2160"/>
    <dbReference type="EnsemblBacteria" id="ABF09039">
        <property type="protein sequence ID" value="ABF09039"/>
        <property type="gene ID" value="Rmet_2160"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2160"/>
    <dbReference type="HOGENOM" id="CLU_1309276_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF09039.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="210" mass="23740" checksum="93D241D1F612FC22" modified="2006-05-30" version="1">MAREMYHGSLSESITVFDDFTHFGTRDAAIAAAAAKAYAQPGSIRPTAYLYCVQIQVSDSQIHKVETGDWGDPTLYSTCRTLLNTQRFPELKDIELQARKIREGRSRQSKIDAEKYLIRAMADALQDRVGAFEYTNKVEDERSQSLLVVRGANVVRHGKVEKIEQSQLEAARVHNPLHHANSPANQFQEVAPHIQARDEFRNQRPSSSRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="111" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LLI2</accession>
    <name>Q1LLI2_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">RNA polymerase sigma factor RpoS</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Sigma S</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="5">Sigma-38</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5 9" type="primary">rpoS</name>
        <name evidence="9" type="ordered locus">Rmet_2115</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is the master transcriptional regulator of the stationary phase and the general stress response.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Interacts with the RNA polymerase core enzyme.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the sigma-70 factor family. RpoS subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08994.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516828.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2115"/>
    <dbReference type="EnsemblBacteria" id="ABF08994">
        <property type="protein sequence ID" value="ABF08994"/>
        <property type="gene ID" value="Rmet_2115"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2115"/>
    <dbReference type="eggNOG" id="COG0568">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014793_3_5_4"/>
    <dbReference type="OMA" id="RRVQREF"/>
    <dbReference type="OrthoDB" id="1565734at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016987">
        <property type="term" value="F:sigma factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006352">
        <property type="term" value="P:DNA-templated transcription, initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00959">
        <property type="entry name" value="Sigma70_RpoS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014284">
        <property type="entry name" value="RNA_pol_sigma-70_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000943">
        <property type="entry name" value="RNA_pol_sigma70"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009042">
        <property type="entry name" value="RNA_pol_sigma70_r1_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007627">
        <property type="entry name" value="RNA_pol_sigma70_r2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007624">
        <property type="entry name" value="RNA_pol_sigma70_r3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007630">
        <property type="entry name" value="RNA_pol_sigma70_r4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013325">
        <property type="entry name" value="RNA_pol_sigma_r2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013324">
        <property type="entry name" value="RNA_pol_sigma_r3/r4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012761">
        <property type="entry name" value="RNA_pol_sigma_RpoS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00140">
        <property type="entry name" value="Sigma70_r1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04542">
        <property type="entry name" value="Sigma70_r2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04539">
        <property type="entry name" value="Sigma70_r3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04545">
        <property type="entry name" value="Sigma70_r4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00046">
        <property type="entry name" value="SIGMA70FCT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88659">
        <property type="entry name" value="SSF88659"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88946">
        <property type="entry name" value="SSF88946"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02394">
        <property type="entry name" value="rpoS_proteo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02937">
        <property type="entry name" value="sigma70-ECF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00715">
        <property type="entry name" value="SIGMA70_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00716">
        <property type="entry name" value="SIGMA70_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0731">Sigma factor</keyword>
    <keyword evidence="4 5" id="KW-0804">Transcription</keyword>
    <keyword evidence="1 5" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="RNA_pol_sigma70" evidence="7">
        <location>
            <begin position="174"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="domain" description="RNA_pol_sigma70" evidence="8">
        <location>
            <begin position="345"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="H-T-H motif" evidence="5">
        <location>
            <begin position="346"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="region of interest" description="Sigma-70 factor domain-1" evidence="5">
        <location>
            <begin position="112"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="region of interest" description="Sigma-70 factor domain-2" evidence="5">
        <location>
            <begin position="150"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="region of interest" description="Sigma-70 factor domain-4" evidence="5">
        <location>
            <begin position="320"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Interaction with polymerase core subunit RpoC" evidence="5">
        <location>
            <begin position="174"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="72"/>
            <end position="94"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00959"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS00715"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS00716"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ABF08994.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="386" mass="43311" checksum="B642E826E584B14E" modified="2006-05-30" version="1">MPRQKTVSSGVVSRTRRPKQPEGKAGVARPDGGIDTEMFGDDPAAELVPVADEPIAPVSRVGLRNTDLNADDADHDDGDEEESEEDEEEGSSETTAEPDDFRTVLHTELAADTVQHYLNRISIKPLLSAPEELHFSTLAKGGDFSARQVMIERNLRLVVSIAKGYLNRGVPLLDLIEEGNLGLMHAIEKFDPSRGFRFSTYATWWIRQSIERAIMNQARTVRLPVHVIRELNQVLRAKRHLEKGGTDGRDASLEDIAHLLGKTPDEVQDVLALNEHTTSLDTPFDLDPGSSLLDFLSDEHNAAPDQEVAHRELENLMKLWLARLSEKHRYVVERRFGLNHIEPATLEELAEEMGLTRERVRQIQQEALVKLKRHFASQGVRKDAVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LM07</accession>
    <name>Q1LM07_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Probable membrane transporter protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Rmet_1940</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cell membrane</location>
            <topology evidence="5">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the 4-toluene sulfonate uptake permease (TSUP) (TC 2.A.102) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08819.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1940"/>
    <dbReference type="EnsemblBacteria" id="ABF08819">
        <property type="protein sequence ID" value="ABF08819"/>
        <property type="gene ID" value="Rmet_1940"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1940"/>
    <dbReference type="eggNOG" id="COG0730">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_054750_5_1_4"/>
    <dbReference type="OMA" id="VLIHFMP"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002781">
        <property type="entry name" value="TM_pro_TauE-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01925">
        <property type="entry name" value="TauE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="43"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="77"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="112"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="137"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="167"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="204"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="262"/>
            <end position="283"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU363041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABF08819.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="284" mass="29817" checksum="3CA748DBA8B99000" modified="2006-05-30" version="1">MPICDGSARHLRSLGPSRGATLNPLPIPASPMDLSALLPDVSLPAYATMSAALAVAYVIFGIAGFGTALMAAPVLAYVMPVAQIVPLLALMDCSAALLNLARDGHAADRTELRRLVPCLLIGSLIGAALLLRIRADLLPAVLGVFVTAYAIWSLLGKRPATRLPSSAAVPFGLVGGICSAMFGSGGFLYAIYLSSRLDKDAMRVTQSTLIGLSTLTRLVLFLLAGVYARAELLKLAVALAPAMIAGLWAGRQITLRMSREQFLRVVNVIVLLSGSALVIRTWLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LMB4</accession>
    <name>Q1LMB4_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Predicted acyltransferase</fullName>
            <ecNumber evidence="2">2.3.1.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_1833</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.3.1.-" evidence="2"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08712.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516562.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1833"/>
    <dbReference type="EnsemblBacteria" id="ABF08712">
        <property type="protein sequence ID" value="ABF08712"/>
        <property type="gene ID" value="Rmet_1833"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1833"/>
    <dbReference type="eggNOG" id="COG2153">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_056607_6_4_4"/>
    <dbReference type="OMA" id="PHVEMRK"/>
    <dbReference type="OrthoDB" id="1777515at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004343">
        <property type="term" value="F:glucosamine 6-phosphate N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006048">
        <property type="term" value="P:UDP-N-acetylglucosamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039143">
        <property type="entry name" value="GNPNAT1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13355">
        <property type="entry name" value="PTHR13355"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13673">
        <property type="entry name" value="Acetyltransf_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="SSF55729"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="1">
        <location>
            <begin position="10"/>
            <end position="147"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF08712.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="147" mass="16146" checksum="517030982FCA60B3" modified="2006-05-30" version="1">MSAFTPTSAPVLVCLWEEARERARAIRYAVFVEEQGVPVELEWDEMDAPSWHALAFAVDGVPVATGRLLPDGHIGRMAVLKSARGTGVGAKVLDALMAKATELGYPELILNAQTHAAPFYTRVGFEQVGDEFEEAGIPHIEMRKRIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LMB8</accession>
    <name>Q1LMB8_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Endolytic murein transglycosylase</fullName>
            <ecNumber evidence="7">4.2.2.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Peptidoglycan polymerization terminase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">yceG</name>
        <name evidence="7" type="synonym">mltG</name>
        <name evidence="8" type="ordered locus">Rmet_1829</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Functions as a peptidoglycan terminase that cleaves nascent peptidoglycan strands endolytically to terminate their elongation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the transglycosylase MltG family.</text>
    </comment>
    <dbReference type="EC" id="4.2.2.-" evidence="7"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08708.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516558.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1829"/>
    <dbReference type="EnsemblBacteria" id="ABF08708">
        <property type="protein sequence ID" value="ABF08708"/>
        <property type="gene ID" value="Rmet_1829"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1829"/>
    <dbReference type="eggNOG" id="COG1559">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025574_0_2_4"/>
    <dbReference type="OMA" id="IAMPGKA"/>
    <dbReference type="OrthoDB" id="708179at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005887">
        <property type="term" value="C:integral component of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008932">
        <property type="term" value="F:lytic endotransglycosylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009252">
        <property type="term" value="P:peptidoglycan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd08010">
        <property type="entry name" value="MltG_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02065">
        <property type="entry name" value="MltG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003770">
        <property type="entry name" value="MLTG-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30518">
        <property type="entry name" value="PTHR30518"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02618">
        <property type="entry name" value="YceG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00247">
        <property type="entry name" value="TIGR00247"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="1 7" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="5 7" id="KW-0456">Lyase</keyword>
    <keyword evidence="4 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="site" description="Important for catalytic activity" evidence="7">
        <location>
            <position position="215"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ABF08708.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="332" mass="36338" checksum="F185A54240D941F1" modified="2006-05-30" version="1">MKRLFLRLVGVLVVFAMAAAGGFAWWAHQPLGLTTSPIEVVIKPNSGVLSVGRQIQRGGVGMDPRLFQILVRITGHGPDLKAGGYQFETGATPLDVIGKLARGEVTHYVVTVIEGWEFRKMRAVVDANPALKHDTVGMSDADLMHAIGAAESAPEGLFFPDTYLFARGSSDIELYKHAYRAMQRRLNEAWNARAPDLPYKTPYEALVMASIVEKETGQAAERPMIAAVFVNRLRKNMMLQTDPTVIYGLGEQFDGNLRKRDLLTDTPYNTYTRTGLPPTPIALPSLASLAAATTPAPSDALYFVARGDGSSHFSNSLPEHNRAVDKYQRGKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="92" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LMD1</accession>
    <name>Q1LMD1_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Acyl-CoA dehydrogenase, short-chain specific</fullName>
            <ecNumber evidence="8">1.3.99.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Rmet_1816</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the acyl-CoA dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.3.99.-" evidence="8"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08695.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1816"/>
    <dbReference type="EnsemblBacteria" id="ABF08695">
        <property type="protein sequence ID" value="ABF08695"/>
        <property type="gene ID" value="Rmet_1816"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1816"/>
    <dbReference type="eggNOG" id="COG1960">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018204_0_2_4"/>
    <dbReference type="OMA" id="YAIPQQW"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003995">
        <property type="term" value="F:acyl-CoA dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.540.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.110.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006089">
        <property type="entry name" value="Acyl-CoA_DH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006091">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046373">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036250">
        <property type="entry name" value="AcylCo_DH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009075">
        <property type="entry name" value="AcylCo_DH/oxidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013786">
        <property type="entry name" value="AcylCoA_DH/ox_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037069">
        <property type="entry name" value="AcylCoA_DH/ox_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009100">
        <property type="entry name" value="AcylCoA_DH/oxidase_NM_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00441">
        <property type="entry name" value="Acyl-CoA_dh_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02770">
        <property type="entry name" value="Acyl-CoA_dh_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02771">
        <property type="entry name" value="Acyl-CoA_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47203">
        <property type="entry name" value="SSF47203"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56645">
        <property type="entry name" value="SSF56645"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00072">
        <property type="entry name" value="ACYL_COA_DH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00073">
        <property type="entry name" value="ACYL_COA_DH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0274">FAD</keyword>
    <keyword evidence="3" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Acyl-CoA_dh_N" evidence="7">
        <location>
            <begin position="52"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA_dh_M" evidence="6">
        <location>
            <begin position="164"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA_dh_1" evidence="5">
        <location>
            <begin position="273"/>
            <end position="420"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF02770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF02771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ABF08695.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="429" mass="46850" checksum="9390801A20B22E50" modified="2006-05-30" version="1">MAFILPKRCADCAMVVQDSETTVPTFGRRAYAPRHQKSWWSETMIRDPEAFDAFLQTLRRFVREKLVPREAEVAEHDEVAQDLVDAMAAQGMFGYSIPEQYGGAGMTTEELVLAAMELSQCSVAFRARVGTNTGIGSEALVADGTPAQKQHYLPLLASGQLTGSFALTEPEAGSDATALQTTAVRDGDAYILNGTKCFITNAPIAGLFTVMARTDPANLGAGGISAFIVPRETPGLSTGRPYQKMGQAGSPVSEVHFDNCRVPAVNLIGGEEGKGFRTAMKVLNKQRIHLAALCTGPAIRMLEDAVRFVTERKQFGQVLADFQLVQAMIADCQTDIHAARALILETARKRDLGEDVTMQASICKYFASEMCGRVADRCVQLFGGYGYIADYGIERFYRDVRLFRLYEGTSQIHQINIAKRTFAQAGGQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="56" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LMF0</accession>
    <name>Q1LMF0_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_1797</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08676.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008651726.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF08676">
        <property type="protein sequence ID" value="ABF08676"/>
        <property type="gene ID" value="Rmet_1797"/>
    </dbReference>
    <dbReference type="GeneID" id="60825709"/>
    <dbReference type="KEGG" id="rme:Rmet_1797"/>
    <dbReference type="HOGENOM" id="CLU_136209_2_0_4"/>
    <dbReference type="OMA" id="VNDRRDP"/>
    <dbReference type="OrthoDB" id="2001065at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193671" evidence="2">
        <location>
            <begin position="24"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="45"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="45"/>
            <end position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF08676.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="105" mass="11008" checksum="C4F823BCC0540455" modified="2006-05-30" version="1">MLKALIATTITTTLLGASALAVAQPPQGLQDRGVPADRYGYEMHIGKRDPYTDGAHGIRQRDPYTDGAHGIRERDPYTDGAHGSAGMNLAGLDRTGVSAPPAHSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-10-07" version="55" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LMI6</accession>
    <name>Q1LMI6_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_1761</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08640.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF08640">
        <property type="protein sequence ID" value="ABF08640"/>
        <property type="gene ID" value="Rmet_1761"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1761"/>
    <dbReference type="HOGENOM" id="CLU_082958_0_0_4"/>
    <dbReference type="OMA" id="CANVPRG"/>
    <dbReference type="OrthoDB" id="1110382at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193563" evidence="1">
        <location>
            <begin position="19"/>
            <end position="218"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF08640.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="218" mass="23579" checksum="226B2D0EF5A6F5F9" modified="2006-05-30" version="1">MHLLRSAALATALLSLMACTTRPFQPSPPLYTLWAKPGVNEAGVRSAMLGCGFVHAGHVDGTTMTSDDYARAQLCMIDNGFVYQDRRIVCTDSPDLPACANVPRGKTFGTDPDFDPTLLKRRPSRPPAYTYWSRPGTDTEGVKRAMAACGYSTVIEPINTMLLNDIAAAELCMIDKQFTYALPANALLCKNPPALPACRNRVIDAVRCCAPPKAAGQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="68" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LML6</accession>
    <name>Q1LML6_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_1731</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08610.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516463.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1731"/>
    <dbReference type="EnsemblBacteria" id="ABF08610">
        <property type="protein sequence ID" value="ABF08610"/>
        <property type="gene ID" value="Rmet_1731"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1731"/>
    <dbReference type="eggNOG" id="COG3246">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_065536_2_0_4"/>
    <dbReference type="OMA" id="AGHYKGP"/>
    <dbReference type="OrthoDB" id="1892901at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019475">
        <property type="term" value="P:L-lysine catabolic process to acetate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008567">
        <property type="entry name" value="KCE_family"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37418">
        <property type="entry name" value="PTHR37418"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05853">
        <property type="entry name" value="BKACE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF08610.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="352" mass="39076" checksum="8495CF9CB7D172E4" modified="2006-05-30" version="1">MQFLDDSLHPENMDKVVITVAPYGPEWMPEDFPEDIPVTMEEQIQKAVDCYNAGATVLHLHVRELDGKGSKRLSKFNELIAGVRAAVPDMIIQVGGSISFAPENEGQAATWLSDDTRHMLADLDPKPDQVTVAINTTQMNIMELLYPEYLEGTSLSNPAYIQAYREMTVPAGPGWVEEHLRRLSASGIQPHFQLTGIHAMETLERMVRAGAYKGPLNLTWIGIGGGFDGPNPFNFFNFIHRAPDGCTLTAESLLKNVLPFNMMAMAMGMHPRCGIEDTIIDQHGNRMTSVQQIEQCVRVAKELGREVASGKEAREIYRIGTWYDSVDETLAAHGMLPNRKSGQKNLPLRKAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="63" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LMT4</accession>
    <name>Q1LMT4_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Putative mRNA interferase YoeB</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Rmet_1659</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the YoeB family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08542.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1659"/>
    <dbReference type="EnsemblBacteria" id="ABF08542">
        <property type="protein sequence ID" value="ABF08542"/>
        <property type="gene ID" value="Rmet_1659"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1659"/>
    <dbReference type="eggNOG" id="COG4115">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_169492_5_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006401">
        <property type="term" value="P:RNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2310.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035093">
        <property type="entry name" value="RelE/ParE_toxin_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009614">
        <property type="entry name" value="YoeB_toxin"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06769">
        <property type="entry name" value="YoeB_toxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143011">
        <property type="entry name" value="SSF143011"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0540">Nuclease</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00030388"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABF08542.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="36" mass="4266" checksum="89B0473B4883B6AA" modified="2006-05-30" version="1">MSGYWSRRIDDTNRLVYFADDTELAIIACRLHYGDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="56" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LNB3</accession>
    <name>Q1LNB3_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_1480</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08363.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516229.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1480"/>
    <dbReference type="EnsemblBacteria" id="ABF08363">
        <property type="protein sequence ID" value="ABF08363"/>
        <property type="gene ID" value="Rmet_1480"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1480"/>
    <dbReference type="eggNOG" id="COG5489">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_109334_1_0_4"/>
    <dbReference type="OMA" id="IWSRRNG"/>
    <dbReference type="OrthoDB" id="1730357at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007948">
        <property type="entry name" value="DUF736"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05284">
        <property type="entry name" value="DUF736"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF08363.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="104" mass="11395" checksum="ECBF85DDED6AD5D8" modified="2006-05-30" version="1">MANIGNFTADKDGFTGTLRTLTLNVKVKLVPNDKGDNEKSPDYRLQAASHDIGAAWRKTSEAGREYISVTLDDPSFPATVYARLIEGEDGTHDLIWSRSKPQAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="64" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LNH5</accession>
    <name>Q1LNH5_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_1418</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08301.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516172.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1418"/>
    <dbReference type="EnsemblBacteria" id="ABF08301">
        <property type="protein sequence ID" value="ABF08301"/>
        <property type="gene ID" value="Rmet_1418"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1418"/>
    <dbReference type="eggNOG" id="COG5455">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_102089_0_1_4"/>
    <dbReference type="OMA" id="YYLVAPN"/>
    <dbReference type="OrthoDB" id="2046853at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024572">
        <property type="entry name" value="RcnB"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11776">
        <property type="entry name" value="RcnB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193238" evidence="2">
        <location>
            <begin position="25"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="27"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="27"/>
            <end position="50"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF08301.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="116" mass="12279" checksum="BDAAB2F5EC806A4A" modified="2006-05-30" version="1">MKTRMLIPIAVAAAGMLAAPFALAQHSGSMKSSGSMSSSPSSSGTAPMGAASPYTPQKWNKGDKLPAEFRDRQYVIDKYKEYNLPAPKKGYHWVGIGADYYQVSSNGTVYSVGPGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LNK5</accession>
    <name>Q1LNK5_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Transcriptional regulator, LysR-family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">lysR</name>
        <name evidence="3" type="ordered locus">Rmet_1388</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the LysR transcriptional regulatory family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08271.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1388"/>
    <dbReference type="EnsemblBacteria" id="ABF08271">
        <property type="protein sequence ID" value="ABF08271"/>
        <property type="gene ID" value="Rmet_1388"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1388"/>
    <dbReference type="eggNOG" id="COG0583">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039613_16_2_4"/>
    <dbReference type="OMA" id="HMTVSAI"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005119">
        <property type="entry name" value="LysR_subst-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000847">
        <property type="entry name" value="Tscrpt_reg_HTH_LysR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00126">
        <property type="entry name" value="HTH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03466">
        <property type="entry name" value="LysR_substrate"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50931">
        <property type="entry name" value="HTH_LYSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH lysR-type" evidence="2">
        <location>
            <begin position="47"/>
            <end position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF08271.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="362" mass="39321" checksum="85DD165159C5C376" modified="2006-05-30" version="1">MNASNCSETLQCGKAKAGHLIFYFCTTNLAQLDLDLCINRRTSVAQFKQLETFVSVASRGSLSAAAAAEGVAPAIIGRRIDALEERLGVKLLVRTTRKITLTFEGSAFLEDCQRILNDLHNAEASVSAGGVKASGHLRITAPAGFGRKHVAPLVPLFIESHPDVTLTLDLSDRVVDLVNEGFDCAIRLGDLPDSSLVSIRLAENRRVVVASPEYLARAGTPTQPEELARHNCLAFGASANVQRGWVFQQDGRATTVKVGGNMECTDGAVLHEWCLGGYGLAWRSWWEVGQEIASGRLVTVLDEFQAPPIGIHAVFPQRKHLPLRVRLFIDHLKNTYGNPAYWRRAEMAQPSTNAGVDITAPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LNR3</accession>
    <name>Q1LNR3_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Phenol hydroxylase P1 protein (Phenol 2-monooxygenase P1 component)(Protein belongs to CMGI-2)</fullName>
            <ecNumber evidence="1">1.14.13.7</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">tomA1</name>
        <name evidence="1" type="ordered locus">Rmet_1330</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EC" id="1.14.13.7" evidence="1"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08213.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516096.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1330"/>
    <dbReference type="EnsemblBacteria" id="ABF08213">
        <property type="protein sequence ID" value="ABF08213"/>
        <property type="gene ID" value="Rmet_1330"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1330"/>
    <dbReference type="eggNOG" id="ENOG502Z7Z9">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_833678_0_0_4"/>
    <dbReference type="OMA" id="QGWIYGF"/>
    <dbReference type="OrthoDB" id="561064at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018662">
        <property type="term" value="F:phenol 2-monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006725">
        <property type="term" value="P:cellular aromatic compound metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01058">
        <property type="entry name" value="AAMH_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.620.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009078">
        <property type="entry name" value="Ferritin-like_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012078">
        <property type="entry name" value="MP_mOase_hydro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003430">
        <property type="entry name" value="Phenol_Hydrox"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012348">
        <property type="entry name" value="RNR-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02332">
        <property type="entry name" value="Phenol_Hydrox"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000040">
        <property type="entry name" value="MMOH_comp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47240">
        <property type="entry name" value="SSF47240"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF08213.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="331" mass="37407" checksum="6440E1A8E55B3883" modified="2006-05-30" version="1">MTIDLKTREIKPLRHTYTHVAQYIGADKAASRYQEGTVGAQPAANFHYRPTWDPEHELFDTSRTAIQMKDWYALKDPRQFYYASWTMTRARQQDAMESNFEFVESRGMIDLVSDEVRQQALSVLVPLRHAAWGANMNNSQICALGYGTTFTAPAMFHAMDNLGVAQYLTRLALVMSGPDLLDEAKQAWMTSPDWQPLRRYVENTLVLQDPVELFVAQNLALDGLLYPMIYGAFVDDYIALNGGSAVAMLTTFMPAWHDESSRWVDAVVKTMATESEDNKALLIHWLRTWEDQAASALLPVAEMALAENGHDALEEVRQRLRARVAKAGIVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LNU0</accession>
    <name>Q1LNU0_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transposase ISRme5 (Copy c)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">tnpA</name>
        <name evidence="2" type="ordered locus">Rmet_1301</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08186.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516068.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF08186">
        <property type="protein sequence ID" value="ABF08186"/>
        <property type="gene ID" value="Rmet_1301"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1301"/>
    <dbReference type="HOGENOM" id="CLU_027402_15_1_4"/>
    <dbReference type="OrthoDB" id="1637053at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001584">
        <property type="entry name" value="Integrase_cat-core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00665">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50994">
        <property type="entry name" value="INTEGRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Integrase catalytic" evidence="1">
        <location>
            <begin position="122"/>
            <end position="302"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF08186.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="321" mass="37272" checksum="2991505B842AC03F" modified="2006-05-30" version="1">MHLRLHKNATTTPRIRAEIQVSKEPMRVLAQRFGVSVSTIARWKKRASVHDASHTPHRLQTTLTPAQESIVLVLRKSLGLSLDDLLAVVREFIHPTVSRAALHRMLKRHGVSAREALSVDRPRTKPFKAYEPGFVHIDVKYLPQMADETTRRYLFVAIDRATRWVFVRVYASKSATNARRFLKELHKAAAFRIRTILTDNGKEFTDRFITRGERTPTGRHQFDQLCEELGIEHRLTRPKHPQTNGMVERFNGRIADILRTHHFHSGEELEATILRYVWLYNHQLPQKALGHVSPIQAMKQWQRSHPELFNRRVTNQPGHDT</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LNZ9</accession>
    <name>Q1LNZ9_CUPMC</name>
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        <submittedName>
            <fullName evidence="7">Tyrosine-based site-specific recombinase, N-terminal SAM-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">ritC2</name>
        <name evidence="7" type="ordered locus">Rmet_1241</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08127.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1241"/>
    <dbReference type="EnsemblBacteria" id="ABF08127">
        <property type="protein sequence ID" value="ABF08127"/>
        <property type="gene ID" value="Rmet_1241"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1241"/>
    <dbReference type="eggNOG" id="COG4974">
        <property type="taxonomic scope" value="Bacteria"/>
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    <dbReference type="OrthoDB" id="745068at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.443.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044068">
        <property type="entry name" value="CB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011010">
        <property type="entry name" value="DNA_brk_join_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013762">
        <property type="entry name" value="Integrase-like_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002104">
        <property type="entry name" value="Integrase_catalytic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010998">
        <property type="entry name" value="Integrase_recombinase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004107">
        <property type="entry name" value="Integrase_SAM-like_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02899">
        <property type="entry name" value="Phage_int_SAM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00589">
        <property type="entry name" value="Phage_integrase"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SSF56349"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="CB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51898">
        <property type="entry name" value="TYR_RECOMBINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="3" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="2 4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Core-binding (CB)" evidence="6">
        <location>
            <begin position="4"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="domain" description="Tyr recombinase" evidence="5">
        <location>
            <begin position="121"/>
            <end position="308"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABF08127.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="336" mass="37916" checksum="07DE54AA5BEE7B60" modified="2006-05-30" version="1">MKDFNLPSIIHRYFLEYLPRHKGLQSSSIRSYRDSLRLFLIFVAGTHRLGVSELVLEHLDYPAVQAFLHSMEADRGNAISTRNQRLAALHVFYEYVGRTVPEMLPTSAKVAAIPMKRCPRPEMTFLGRDEVQAMFACIPAQHRLSSRDRALLMLLYNTGARVSEVAHLKVEQLDLRSPARVRLLGKGSKWRTCPLWNQTAKALQAMLDERGTNLPPESPVFVGASQAPMTRFGIYKRIRHYATLWEASGHTASAAKVTPHVFRHTTAVHLLESGVEVNVIRGWLGHVNLETTNRYAEITIRMKAEALKLCDPIVAGVPRKPAWKDDAAMMAWLSSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LP08</accession>
    <name>Q1LP08_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative RNA-binding transcription accessory protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">yhgF</name>
        <name evidence="3" type="ordered locus">Rmet_1232</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08118.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516006.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1232"/>
    <dbReference type="EnsemblBacteria" id="ABF08118">
        <property type="protein sequence ID" value="ABF08118"/>
        <property type="gene ID" value="Rmet_1232"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1232"/>
    <dbReference type="eggNOG" id="COG2183">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009833_0_2_4"/>
    <dbReference type="OMA" id="RWAWRTR"/>
    <dbReference type="OrthoDB" id="120764at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006139">
        <property type="term" value="P:nucleobase-containing compound metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05685">
        <property type="entry name" value="S1_Tex"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.650">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3500.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041692">
        <property type="entry name" value="HHH_9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010994">
        <property type="entry name" value="RuvA_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022967">
        <property type="entry name" value="S1_dom"/>
    </dbReference>
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        <property type="entry name" value="S1_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044146">
        <property type="entry name" value="S1_Tex"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023323">
        <property type="entry name" value="Tex-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023319">
        <property type="entry name" value="Tex-like_HTH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018974">
        <property type="entry name" value="Tex-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032639">
        <property type="entry name" value="Tex_YqgF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006641">
        <property type="entry name" value="YqgF/RNaseH-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037027">
        <property type="entry name" value="YqgF/RNaseH-like_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17674">
        <property type="entry name" value="HHH_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00575">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09371">
        <property type="entry name" value="Tex_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16921">
        <property type="entry name" value="Tex_YqgF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00316">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00732">
        <property type="entry name" value="YqgFc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47781">
        <property type="entry name" value="SSF47781"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="SSF53098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50126">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="S1 motif" evidence="2">
        <location>
            <begin position="663"/>
            <end position="732"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="733"/>
            <end position="787"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="753"/>
            <end position="771"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF08118.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="787" mass="85628" checksum="8984400FEFB45BF5" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="66" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LP40</accession>
    <name>Q1LP40_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Phasin (PHA-granule associated protein)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">phaP</name>
        <name evidence="2" type="ordered locus">Rmet_1200</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08086.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515979.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1200"/>
    <dbReference type="EnsemblBacteria" id="ABF08086">
        <property type="protein sequence ID" value="ABF08086"/>
        <property type="gene ID" value="Rmet_1200"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1200"/>
    <dbReference type="eggNOG" id="COG5490">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_112006_2_1_4"/>
    <dbReference type="OMA" id="YSRHLYD"/>
    <dbReference type="OrthoDB" id="1551074at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018968">
        <property type="entry name" value="Phasin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010127">
        <property type="entry name" value="Phasin_subfam-1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09361">
        <property type="entry name" value="Phasin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01841">
        <property type="entry name" value="phasin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Phasin_2" evidence="1">
        <location>
            <begin position="6"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF09361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF08086.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="192" mass="19964" checksum="A809107C20471E05" modified="2006-05-30" version="1">MILTPEQVAAAQKANLETLFGLTSKAFEGVEKLVELNLQVVKATLAENADNAKKALTAKDAQELLAIQASLVQPVAEKTLAYTRHLYEIASETQSEFTKVAEAQLAEGTKNVQALVDNLAKNAPAGSESTVAIVKSAISAANNAYESVQKATKQAVEMAESNFQAAATAATKAAQQASATARTATKKSAAAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LP90</accession>
    <name>Q1LP90_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">TRAP dicarboxylate transporter, DctP subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_1150</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08036.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515937.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1150"/>
    <dbReference type="EnsemblBacteria" id="ABF08036">
        <property type="protein sequence ID" value="ABF08036"/>
        <property type="gene ID" value="Rmet_1150"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1150"/>
    <dbReference type="eggNOG" id="COG1638">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036176_1_3_4"/>
    <dbReference type="OMA" id="FRIMPSE"/>
    <dbReference type="OrthoDB" id="752834at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030288">
        <property type="term" value="C:outer membrane-bounded periplasmic space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071702">
        <property type="term" value="P:organic substance transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018389">
        <property type="entry name" value="DctP_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004682">
        <property type="entry name" value="TRAP_DctP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038404">
        <property type="entry name" value="TRAP_DctP_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33376">
        <property type="entry name" value="PTHR33376"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03480">
        <property type="entry name" value="DctP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006470">
        <property type="entry name" value="DctB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00787">
        <property type="entry name" value="dctP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193382" evidence="2">
        <location>
            <begin position="27"/>
            <end position="343"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF08036.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="343" mass="37336" checksum="D9913A7411AD4004" modified="2006-05-30" version="1">MKRRALMLSVAASIAAAALAPAGAMAQTYKSEYKMSLVLGPAFPWGKGGEIWADLVRQRTNGRINIKLYPGTSLVAGDQTREFSAIRQGVIDMAVGSTINWSPQVKELNLFSLPFLMPDYKALDSLTQGDVGKSIFATLEKAGVVPLAWGENGFREVSNSKHEIRKPEDLKGLKLRVVGSPLYIETFNALGANPTQMSWADAQPAMASGAVDGQENPQSVFAAAKLYTVGQKFVTTWGYVADPLIFVVNKQIWESWTPADREIVKQAAIDAGKQEIALARKGLNEAGAPAWKDMEQHGVKVTSLTPAEHDAFRKATTKVYDKWKKQIGADLVTKAEASIANRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LP97</accession>
    <name>Q1LP97_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Putative fusion protein (CycG-torC like aminoterminal part)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Rmet_1143</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08029.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1143"/>
    <dbReference type="EnsemblBacteria" id="ABF08029">
        <property type="protein sequence ID" value="ABF08029"/>
        <property type="gene ID" value="Rmet_1143"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1143"/>
    <dbReference type="eggNOG" id="COG2010">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG3005">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_499552_0_0_4"/>
    <dbReference type="OMA" id="FHMPNIS"/>
    <dbReference type="OrthoDB" id="1297285at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.760.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009056">
        <property type="entry name" value="Cyt_c-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036909">
        <property type="entry name" value="Cyt_c-like_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00034">
        <property type="entry name" value="Cytochrom_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46626">
        <property type="entry name" value="SSF46626"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51007">
        <property type="entry name" value="CYTC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 4" id="KW-0349">Heme</keyword>
    <keyword evidence="3 4" id="KW-0408">Iron</keyword>
    <keyword evidence="2 4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cytochrome c" evidence="5">
        <location>
            <begin position="45"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="domain" description="Cytochrome c" evidence="5">
        <location>
            <begin position="194"/>
            <end position="303"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABF08029.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="532" mass="57556" checksum="B8D3CF664F761DD6" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LPR8</accession>
    <name>Q1LPR8_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative aminoglycoside phosphotransferase</fullName>
            <ecNumber evidence="2">2.7.1.95</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_0972</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.7.1.95" evidence="2"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07858.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515776.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0972"/>
    <dbReference type="EnsemblBacteria" id="ABF07858">
        <property type="protein sequence ID" value="ABF07858"/>
        <property type="gene ID" value="Rmet_0972"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0972"/>
    <dbReference type="eggNOG" id="COG3173">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007526_0_1_4"/>
    <dbReference type="OMA" id="FVDYYAE"/>
    <dbReference type="OrthoDB" id="1457558at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008910">
        <property type="term" value="F:kanamycin kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd05154">
        <property type="entry name" value="ACAD10_11_N-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041726">
        <property type="entry name" value="ACAD10_11_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002575">
        <property type="entry name" value="Aminoglycoside_PTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01636">
        <property type="entry name" value="APH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="SSF56112"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="APH" evidence="1">
        <location>
            <begin position="41"/>
            <end position="277"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01636"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF07858.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="358" mass="40270" checksum="ABE0DF9E6F4B21E4" modified="2006-05-30" version="1">MSTNVSHFEGTRPVADQQRFDVGALESWMREHVDGFAGPLTVEQFKGGQSNPTFKLITPGQTYVMRAKPGPKAKLLPSAHAIEREYRVMAALAGTDVPVARMFALCEDEEVIGRAFYIMEFVGGRVLWDQSLPDMTKSERGAIYDEMNRVIAALHTVDYKAIGLADYGKPGNYFQRQIERWSKQYKLSETESIPAMDQLMAWLPDHIPQEDVDLTSIVHGDYRLDNLMFHPTEPKVLAVLDWELSTLGHPMADFGYHCMSWHIQPGQFRGIAGLDYQALGIPDEATYRRLYEQRTGRQITGDWNFYLAFSMFRIAGILQGIMKRVVDGTASSAQATDAGKRARPMAEMGWAYAQKSGA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-12-15" modified="2022-05-25" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LPV3</accession>
    <name>NUOK_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">NADH-quinone oxidoreductase subunit K</fullName>
            <ecNumber evidence="1">7.1.1.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">NADH dehydrogenase I subunit K</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">NDH-1 subunit K</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">nuoK</name>
        <name type="ordered locus">Rmet_0937</name>
    </gene>
    <organism>
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a quinone + 5 H(+)(in) + NADH = a quinol + 4 H(+)(out) + NAD(+)</text>
            <dbReference type="Rhea" id="RHEA:57888"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:24646"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:132124"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">NDH-1 is composed of 14 different subunits. Subunits NuoA, H, J, K, L, M, N constitute the membrane sector of the complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the complex I subunit 4L family.</text>
    </comment>
    <dbReference type="EC" id="7.1.1.-" evidence="1"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07823.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008643358.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q1LPV3"/>
    <dbReference type="SMR" id="Q1LPV3"/>
    <dbReference type="STRING" id="266264.Rmet_0937"/>
    <dbReference type="EnsemblBacteria" id="ABF07823">
        <property type="protein sequence ID" value="ABF07823"/>
        <property type="gene ID" value="Rmet_0937"/>
    </dbReference>
    <dbReference type="GeneID" id="60825424"/>
    <dbReference type="KEGG" id="rme:Rmet_0937"/>
    <dbReference type="eggNOG" id="COG0713">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_144724_2_0_4"/>
    <dbReference type="OMA" id="NFVAFSY"/>
    <dbReference type="OrthoDB" id="2081663at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050136">
        <property type="term" value="F:NADH dehydrogenase (quinone) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048038">
        <property type="term" value="F:quinone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042773">
        <property type="term" value="P:ATP synthesis coupled electron transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01456">
        <property type="entry name" value="NDH1_NuoK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001133">
        <property type="entry name" value="NADH_UbQ_OxRdtase_chain4L/K"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039428">
        <property type="entry name" value="NUOK/Mnh_C1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11434">
        <property type="entry name" value="PTHR11434"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00420">
        <property type="entry name" value="Oxidored_q2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0997">Cell inner membrane</keyword>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-0520">NAD</keyword>
    <keyword id="KW-0874">Quinone</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-1278">Translocase</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <keyword id="KW-0813">Transport</keyword>
    <keyword id="KW-0830">Ubiquinone</keyword>
    <feature type="chain" id="PRO_0000390183" description="NADH-quinone oxidoreductase subunit K">
        <location>
            <begin position="1"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="4"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="30"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="61"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01456"/>
        </source>
    </evidence>
    <sequence length="101" mass="10969" checksum="EC4B6EEE4670707C" modified="2006-05-30" version="1">MLSLAHFLVLGAILFAISIVGIFLNRKNVIVLLMALELLLLAVNMNFVAFSHYMGDLAGQVFVFFILTVAAAESAIGLAILVVLFRNLDTINVDDMDTLKG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-10-17" modified="2022-08-03" version="99" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LPX7</accession>
    <name>ILVC_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Ketol-acid reductoisomerase (NADP(+))</fullName>
            <shortName evidence="1">KARI</shortName>
            <ecNumber evidence="1">1.1.1.86</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Acetohydroxy-acid isomeroreductase</fullName>
            <shortName evidence="1">AHIR</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Alpha-keto-beta-hydroxylacyl reductoisomerase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Ketol-acid reductoisomerase type 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Ketol-acid reductoisomerase type I</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">ilvC</name>
        <name type="ordered locus">Rmet_0913</name>
    </gene>
    <organism>
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in the biosynthesis of branched-chain amino acids (BCAA). Catalyzes an alkyl-migration followed by a ketol-acid reduction of (S)-2-acetolactate (S2AL) to yield (R)-2,3-dihydroxy-isovalerate. In the isomerase reaction, S2AL is rearranged via a Mg-dependent methyl migration to produce 3-hydroxy-3-methyl-2-ketobutyrate (HMKB). In the reductase reaction, this 2-ketoacid undergoes a metal-dependent reduction by NADPH to yield (R)-2,3-dihydroxy-isovalerate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(2R)-2,3-dihydroxy-3-methylbutanoate + NADP(+) = (2S)-2-acetolactate + H(+) + NADPH</text>
            <dbReference type="Rhea" id="RHEA:22068"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:49072"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:58476"/>
            <dbReference type="EC" id="1.1.1.86"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(2R,3R)-2,3-dihydroxy-3-methylpentanoate + NADP(+) = (S)-2-ethyl-2-hydroxy-3-oxobutanoate + H(+) + NADPH</text>
            <dbReference type="Rhea" id="RHEA:13493"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:49256"/>
            <dbReference type="ChEBI" id="CHEBI:49258"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.1.1.86"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="1">Binds 2 magnesium ions per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 2/4.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 2/4.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the ketol-acid reductoisomerase family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.86" evidence="1"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07799.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515738.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q1LPX7"/>
    <dbReference type="SMR" id="Q1LPX7"/>
    <dbReference type="STRING" id="266264.Rmet_0913"/>
    <dbReference type="PRIDE" id="Q1LPX7"/>
    <dbReference type="EnsemblBacteria" id="ABF07799">
        <property type="protein sequence ID" value="ABF07799"/>
        <property type="gene ID" value="Rmet_0913"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0913"/>
    <dbReference type="eggNOG" id="COG0059">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033821_0_1_4"/>
    <dbReference type="OMA" id="RAMFSWL"/>
    <dbReference type="OrthoDB" id="188901at2"/>
    <dbReference type="UniPathway" id="UPA00047">
        <property type="reaction ID" value="UER00056"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00049">
        <property type="reaction ID" value="UER00060"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004455">
        <property type="term" value="F:ketol-acid reductoisomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009097">
        <property type="term" value="P:isoleucine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009099">
        <property type="term" value="P:valine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00435">
        <property type="entry name" value="IlvC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008927">
        <property type="entry name" value="6-PGluconate_DH-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013023">
        <property type="entry name" value="KARI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000506">
        <property type="entry name" value="KARI_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013116">
        <property type="entry name" value="KARI_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014359">
        <property type="entry name" value="KARI_prok"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21371">
        <property type="entry name" value="PTHR21371"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01450">
        <property type="entry name" value="IlvC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07991">
        <property type="entry name" value="IlvN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000116">
        <property type="entry name" value="IlvC_gammaproteo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48179">
        <property type="entry name" value="SSF48179"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00465">
        <property type="entry name" value="ilvC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51851">
        <property type="entry name" value="KARI_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51850">
        <property type="entry name" value="KARI_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0100">Branched-chain amino acid biosynthesis</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0521">NADP</keyword>
    <keyword id="KW-0560">Oxidoreductase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000252775" description="Ketol-acid reductoisomerase (NADP(+))">
        <location>
            <begin position="1"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="domain" description="KARI N-terminal Rossmann" evidence="2">
        <location>
            <begin position="1"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="domain" description="KARI C-terminal knotted" evidence="3">
        <location>
            <begin position="182"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="107"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="24"/>
            <end position="27"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="47"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="52"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="133"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="190"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="190"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="194"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="226"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="230"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="251"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01197"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01198"/>
        </source>
    </evidence>
    <sequence length="338" mass="36444" checksum="D63E49A87A265C40" modified="2006-05-30" version="1">MKVFYDKDADLSLIKGKNVTIIGYGSQGHAHALNLKDSGVNVTVGLRKSGASWNKAVNAGLQVKEVAEAVKGADVVMILLPDEQIADVYKNEVHANIKEGAALAFAHGFNVHYGAVIPRADLDVIMIAPKAPGHTVRSTYAQGGGVPHLIAVHQNKSGAARDIALSYATANGGGRAGIIETNFREETETDLFGEQAVLCGGTVELIKAGFETLVEAGYAPEMAYFECLHELKLIVDLIYEGGIANMNYSISNNAEYGEYVTGPRVVTEETKKAMKQCLKDIQTGEYAKSFLLENKAGAPTLISRRRLTAEHQIEEVGGKLRAMMPWIAKNKLVDQSKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="97" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LQ05</accession>
    <name>Q1LQ05_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">5-hydroxyisourate hydrolase</fullName>
            <shortName evidence="7">HIU hydrolase</shortName>
            <shortName evidence="7">HIUHase</shortName>
            <ecNumber evidence="7">3.5.2.17</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">Rmet_0885</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Catalyzes the hydrolysis of 5-hydroxyisourate (HIU) to 2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline (OHCU).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 7">
            <text>5-hydroxyisourate + H2O = 5-hydroxy-2-oxo-4-ureido-2,5-dihydro-1H-imidazole-5-carboxylate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:23736"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:18072"/>
            <dbReference type="ChEBI" id="CHEBI:58639"/>
            <dbReference type="EC" id="3.5.2.17"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="7">Homotetramer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 7">Belongs to the transthyretin family. 5-hydroxyisourate hydrolase subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.5.2.17" evidence="7"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07771.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515711.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0885"/>
    <dbReference type="EnsemblBacteria" id="ABF07771">
        <property type="protein sequence ID" value="ABF07771"/>
        <property type="gene ID" value="Rmet_0885"/>
    </dbReference>
    <dbReference type="GeneID" id="60825373"/>
    <dbReference type="KEGG" id="rme:Rmet_0885"/>
    <dbReference type="eggNOG" id="COG2351">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_115536_1_1_4"/>
    <dbReference type="OMA" id="ENQNYHI"/>
    <dbReference type="OrthoDB" id="1955791at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033971">
        <property type="term" value="F:hydroxyisourate hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006144">
        <property type="term" value="P:purine nucleobase metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05822">
        <property type="entry name" value="TLP_HIUase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.180">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014306">
        <property type="entry name" value="Hydroxyisourate_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023418">
        <property type="entry name" value="Thyroxine_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000895">
        <property type="entry name" value="Transthyretin/HIU_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023416">
        <property type="entry name" value="Transthyretin/HIU_hydrolase_d"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036817">
        <property type="entry name" value="Transthyretin/HIU_hydrolase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023419">
        <property type="entry name" value="Transthyretin_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10395">
        <property type="entry name" value="PTHR10395"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00576">
        <property type="entry name" value="Transthyretin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00189">
        <property type="entry name" value="TRNSTHYRETIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49472">
        <property type="entry name" value="SSF49472"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02962">
        <property type="entry name" value="hdxy_isourate"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00768">
        <property type="entry name" value="TRANSTHYRETIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00769">
        <property type="entry name" value="TRANSTHYRETIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 7" id="KW-0659">Purine metabolism</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TR_THY" evidence="8">
        <location>
            <begin position="4"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="7"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="45"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="114"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022631"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR600895-51"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU361270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ABF07771.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="117" mass="12729" checksum="17FA20778451D600" modified="2006-05-30" version="1">MGRLTTHVLDTAAGTPGAAMSITLHKIVDNRRETLKKVTTNHDGRCDQPLLEGADLVAGVYELEFGAGDYFRAQGVKLPEPAFLDVVTLRFGIADTAAHYHVPLLVSPWSYSTYRGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="67" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LQ61</accession>
    <name>Q1LQ61_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Hypothetical membrane protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_0829</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07715.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515658.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0829"/>
    <dbReference type="DNASU" id="4037620"/>
    <dbReference type="EnsemblBacteria" id="ABF07715">
        <property type="protein sequence ID" value="ABF07715"/>
        <property type="gene ID" value="Rmet_0829"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0829"/>
    <dbReference type="eggNOG" id="COG3556">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_092231_2_0_4"/>
    <dbReference type="OMA" id="WAIPLRK"/>
    <dbReference type="OrthoDB" id="1993992at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018706">
        <property type="entry name" value="DUF2214_membrane"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09980">
        <property type="entry name" value="DUF2214"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="47"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="79"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="131"/>
            <end position="150"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF07715.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="151" mass="17137" checksum="F6B9CC01C7A2D9F9" modified="2006-05-30" version="1">MLTDALLAFLHFTAIFVLVTLMAAEAVVLRPDMPPSAVRRLSQYDFFYFLSAMVVLATGLLRLFYGAKGVDFYLHNPWFHAKITVFVLIALATLPPTFAFARWRKQSLKLPEFVPTPAEIKKVRRWVMIEAHAFILLPLCAVMMARGIGIH</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="98" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LQ99</accession>
    <name>Q1LQ99_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Dehydrogenase with different specificities (Short-chain alcohol dehydrogenases)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_0791</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07677.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0791"/>
    <dbReference type="EnsemblBacteria" id="ABF07677">
        <property type="protein sequence ID" value="ABF07677"/>
        <property type="gene ID" value="Rmet_0791"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0791"/>
    <dbReference type="eggNOG" id="COG1028">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010194_1_2_4"/>
    <dbReference type="OMA" id="DNTDEQW"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF07677.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="238" mass="25153" checksum="52540B660F5D7E43" modified="2006-05-30" version="1">MCRCARGGRRGCGDRGAEALEKAAADLRARHGRRVLAVATDITTAEGRKLALDAATKLGDLDILVNNAGGPPPGNFRDWGRDEWLKALDANMLTPIELIKATVDGMIARRWGRIINITSGAVKAPIDVLGLSNGARSGLTGFVAGLAREVAQHGVTVNNLLPGPFNTDRLYKTMEGGAQKAGISVDEMAQRRAASNPTRRFGDPAEFGATCAFLCSRQAAYMTGQNVLLDGGAYPGTF</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-10-07" version="58" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LQA3</accession>
    <name>Q1LQA3_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_0787</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07673.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF07673">
        <property type="protein sequence ID" value="ABF07673"/>
        <property type="gene ID" value="Rmet_0787"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0787"/>
    <dbReference type="eggNOG" id="ENOG5032PRY">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_208241_0_0_4"/>
    <dbReference type="OMA" id="RMFARKM"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021494">
        <property type="entry name" value="DUF3149"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11346">
        <property type="entry name" value="DUF3149"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="31"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF07673.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="50" mass="5406" checksum="94D181AF99541351" modified="2006-05-30" version="1">MEAIKILFTTPTGLGSLAVIAFIIGMGAFFARMFARKMREDAEAARRGGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LQC2</accession>
    <name>Q1LQC2_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Metal-dependent hydrolase involved in phosphonate metabolism</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">phnM</name>
        <name evidence="3" type="ordered locus">Rmet_0768</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07654.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515609.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0768"/>
    <dbReference type="EnsemblBacteria" id="ABF07654">
        <property type="protein sequence ID" value="ABF07654"/>
        <property type="gene ID" value="Rmet_0768"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0768"/>
    <dbReference type="eggNOG" id="COG3454">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_060303_1_0_4"/>
    <dbReference type="OMA" id="SWEPGLR"/>
    <dbReference type="OrthoDB" id="1026967at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016810">
        <property type="term" value="F:hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019700">
        <property type="term" value="P:organic phosphonate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006680">
        <property type="entry name" value="Amidohydro-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011059">
        <property type="entry name" value="Metal-dep_hydrolase_composite"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012696">
        <property type="entry name" value="PhnM"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01979">
        <property type="entry name" value="Amidohydro_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038971">
        <property type="entry name" value="PhnM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51338">
        <property type="entry name" value="SSF51338"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="SSF51556"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Amidohydro-rel" evidence="2">
        <location>
            <begin position="239"/>
            <end position="368"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF07654.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="407" mass="43010" checksum="107355EB0E982E86" modified="2006-05-30" version="1">MHTISQASSTPLAGITGRRVMGADGIGLAMLGIHNGSVMAQPPASGPCIDAGDLLVLPGIVDIHGDAFERSVMPRPGVSFPYGAALLDVDRQLLAHGITTEFHGVTLSWEGGLRGEAYALRMFDALAQLRPVLGAEHKVHLRFEAYHLAGVETALSWMADGRVALLAINDHLPTMARRMSDERKLVQYAERAECDTETFRNRLRAASRNAQEVPAAMSCLIAAALAANLPVASHDDPDVATRQHYHAQGCRIAEFPLTVEAATLARQIGDETVFGAPNVLRGKSHTGAPNATEMVAAGLCSVLASDYYYPALLQAAFKLAQLGVCSLPEAWHLVSRNPARATGLRDRGVLAPGMRADAILVDDSQLGLPRVCATIVNGEVRHASGALSMTDLADLADVAGARGSLAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LQF2</accession>
    <name>Q1LQF2_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Electron transfer flavoprotein subunit alpha (Alpha-ETF) (Electron transfer flavoprotein large subunit) (ETFLS)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">etfA</name>
        <name evidence="7" type="ordered locus">Rmet_0738</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ETF alpha-subunit/FixB family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07624.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008643684.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0738"/>
    <dbReference type="EnsemblBacteria" id="ABF07624">
        <property type="protein sequence ID" value="ABF07624"/>
        <property type="gene ID" value="Rmet_0738"/>
    </dbReference>
    <dbReference type="GeneID" id="60825225"/>
    <dbReference type="KEGG" id="rme:Rmet_0738"/>
    <dbReference type="eggNOG" id="COG2025">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_034178_0_0_4"/>
    <dbReference type="OMA" id="WRPYAEQ"/>
    <dbReference type="OrthoDB" id="1400253at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01715">
        <property type="entry name" value="ETF_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029035">
        <property type="entry name" value="DHS-like_NAD/FAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014730">
        <property type="entry name" value="ETF_a/b_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001308">
        <property type="entry name" value="ETF_a/FixB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033947">
        <property type="entry name" value="ETF_alpha_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014731">
        <property type="entry name" value="ETF_asu_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018206">
        <property type="entry name" value="ETF_asu_C_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43153">
        <property type="entry name" value="PTHR43153"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01012">
        <property type="entry name" value="ETF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00766">
        <property type="entry name" value="ETF_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000089">
        <property type="entry name" value="Electra_flavoP_a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00893">
        <property type="entry name" value="ETF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52467">
        <property type="entry name" value="SSF52467"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00696">
        <property type="entry name" value="ETF_ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0249">Electron transport</keyword>
    <keyword evidence="4" id="KW-0274">FAD</keyword>
    <keyword evidence="3" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ETF" evidence="6">
        <location>
            <begin position="3"/>
            <end position="182"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005817"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABF07624.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="311" mass="31355" checksum="A2AFD4EE227CE23F" modified="2006-05-30" version="1">MTALVIAEHDNQSIKAATLNTVTAAAQCGGDVHVLVAGSNAKAAADAAAKIAGVSKVLLADAPYFGDGLAENLAEQILAVASDYSHILAPATASGKNVLPRVAAKLDVAQLSDITKVDSPDTFERPIYAGNAIAIVKSSDKIKVITVRGTGFDAAAAEGGSASVDTIPVVADAGISQFVSREVTKSDRPELTAAKIIVSGGRGVGSGENYTKVLTPLADKLGAALGASRAAVDAGFVPNDYQVGQTGKIVAPQLYIAVGISGAIQHLAGMKDSKVIVAINKDAEAPIFSVADYGLVGDLNTVVPELVAALG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-04-14" modified="2022-08-03" version="95" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LQG7</accession>
    <name>IHFB_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Integration host factor subunit beta</fullName>
            <shortName evidence="1">IHF-beta</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">ihfB</name>
        <name evidence="1" type="synonym">himD</name>
        <name type="ordered locus">Rmet_0723</name>
    </gene>
    <organism>
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">This protein is one of the two subunits of integration host factor, a specific DNA-binding protein that functions in genetic recombination as well as in transcriptional and translational control.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Heterodimer of an alpha and a beta chain.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the bacterial histone-like protein family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07609.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515571.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q1LQG7"/>
    <dbReference type="SMR" id="Q1LQG7"/>
    <dbReference type="STRING" id="266264.Rmet_0723"/>
    <dbReference type="EnsemblBacteria" id="ABF07609">
        <property type="protein sequence ID" value="ABF07609"/>
        <property type="gene ID" value="Rmet_0723"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0723"/>
    <dbReference type="eggNOG" id="COG0776">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_105066_2_0_4"/>
    <dbReference type="OMA" id="RDRVNIY"/>
    <dbReference type="OrthoDB" id="1847472at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030527">
        <property type="term" value="F:structural constituent of chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006417">
        <property type="term" value="P:regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.520.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00381">
        <property type="entry name" value="IHF_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000119">
        <property type="entry name" value="Hist_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010992">
        <property type="entry name" value="IHF-like_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005685">
        <property type="entry name" value="IHF_beta"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33175">
        <property type="entry name" value="PTHR33175"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00216">
        <property type="entry name" value="Bac_DNA_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01727">
        <property type="entry name" value="DNABINDINGHU"/>
    </dbReference>
    <dbReference type="SMART" id="SM00411">
        <property type="entry name" value="BHL"/>
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    </dbReference>
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        <property type="entry name" value="hip"/>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0233">DNA recombination</keyword>
    <keyword id="KW-0238">DNA-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0804">Transcription</keyword>
    <keyword id="KW-0805">Transcription regulation</keyword>
    <keyword id="KW-0810">Translation regulation</keyword>
    <feature type="chain" id="PRO_1000122229" description="Integration host factor subunit beta">
        <location>
            <begin position="1"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="83"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="107"/>
            <end position="126"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00381"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="135" mass="14683" checksum="5BEE161C3F476523" modified="2006-05-30" version="1">MTKSELVEKLAARFPQLLLRDADISVKTILDAMSEALADGHRIEIRGFGSFGLNKRPPRVGRNPKSGERVLVPEKRVPHFKAGKELRERVDRTVTQGGGMNGNGHAPHGKTGQSQLGSQSPASLHDDGQLNLVRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-10-07" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LQI2</accession>
    <name>Q1LQI2_CUPMC</name>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_0708</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07594.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0708"/>
    <dbReference type="EnsemblBacteria" id="ABF07594">
        <property type="protein sequence ID" value="ABF07594"/>
        <property type="gene ID" value="Rmet_0708"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0708"/>
    <dbReference type="HOGENOM" id="CLU_2168941_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="45"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF07594.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="110" mass="12212" checksum="F0533DC1632250B3" modified="2006-05-30" version="1">MTAFFAENLKNRNRFTQDPAKKQHLANHTPQGPRHRWGPSSTAPRRMPLEANVARALINDGLTNFIRGPYVELSHQVSRLLPLDCPVDLPLELPLPPKRALALGASLSTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LQL8</accession>
    <name>Q1LQL8_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Type IV pilus transmembrane protein PilJ</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">pilJ</name>
        <name evidence="11" type="ordered locus">Rmet_0672</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
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                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
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        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the methyl-accepting chemotaxis (MCP) protein family.</text>
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    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0672"/>
    <dbReference type="EnsemblBacteria" id="ABF07558">
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        <property type="gene ID" value="Rmet_0672"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0672"/>
    <dbReference type="eggNOG" id="COG0840">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
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    <dbReference type="OMA" id="NTIHGMD"/>
    <dbReference type="OrthoDB" id="477199at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003660">
        <property type="entry name" value="HAMP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004089">
        <property type="entry name" value="MCPsignal_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029095">
        <property type="entry name" value="NarX-like_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00015">
        <property type="entry name" value="MCPsignal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13675">
        <property type="entry name" value="PilJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00283">
        <property type="entry name" value="MA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50111">
        <property type="entry name" value="CHEMOTAXIS_TRANSDUC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50885">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0807">Transducer</keyword>
    <keyword evidence="2 8 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="64"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="351"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="domain" description="HAMP" evidence="10">
        <location>
            <begin position="404"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="domain" description="Methyl-accepting transducer" evidence="9">
        <location>
            <begin position="460"/>
            <end position="696"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="380"/>
            <end position="407"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00029447"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ABF07558.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="733" mass="78096" checksum="5211EA427EEA458A" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="99" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LQV0</accession>
    <name>Q1LQV0_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Sodium:dicarboxylate symporter: DAACS family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">gltP2</name>
        <name evidence="8" type="ordered locus">Rmet_0590</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07476.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515450.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0590"/>
    <dbReference type="EnsemblBacteria" id="ABF07476">
        <property type="protein sequence ID" value="ABF07476"/>
        <property type="gene ID" value="Rmet_0590"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0590"/>
    <dbReference type="eggNOG" id="COG1301">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019375_7_0_4"/>
    <dbReference type="OMA" id="KPVPCYN"/>
    <dbReference type="OrthoDB" id="781228at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015293">
        <property type="term" value="F:symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3860.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001991">
        <property type="entry name" value="Na-dicarboxylate_symporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036458">
        <property type="entry name" value="Na:dicarbo_symporter_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42865">
        <property type="entry name" value="PTHR42865"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00375">
        <property type="entry name" value="SDF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF118215">
        <property type="entry name" value="SSF118215"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="53"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="83"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="152"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="194"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="229"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="314"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="352"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="410"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="419"/>
            <end position="435"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ABF07476.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="435" mass="45557" checksum="1CAC51795FD2F1C6" modified="2006-05-30" version="1">MTKRLPTLIFVAMLLGVLVGAAVHNMSPDAATAKSIADHLSILTDVFLRMIKMIIGPLVFATLVSGIASMGDGKTVGRIGLKAMAWFMAASITSLLLGLLMANLLAPGHGMNLPLPAADAASNLKTGALNLRDFIAHMFPKSFVEAMATNEVLQIVVFSLFFGFALGTLKDGVGKPVLAGIDGLGHIMLKVTNYVMAFAPLGVFGAVSAVISAQGLGVLVVYAKLLGSVYAALALLWLALIAGGYFFLGRDVFRLMRTLRTPLMIGFATASSESAYPKVIDQLTRFGVKPRITNFVLPLGYSFNLDGSIMYTSFAALFVAQVYGIQLSLTQQITMLLVLLVTSKGIAGVPRASLVVVAAVLPMFGLPEAGILLVLGIDHILDMGRTVTNVLGNAIATTVVAKSEGAIGAPELSEEADTSEDSNTGLTPAQVLASK</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="101" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LQW5</accession>
    <name>Q1LQW5_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Periplasmic chaperone</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">fimC</name>
        <name evidence="8" type="ordered locus">Rmet_0575</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Periplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the periplasmic pilus chaperone family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07461.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515436.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0575"/>
    <dbReference type="EnsemblBacteria" id="ABF07461">
        <property type="protein sequence ID" value="ABF07461"/>
        <property type="gene ID" value="Rmet_0575"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0575"/>
    <dbReference type="eggNOG" id="COG3121">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_070768_0_2_4"/>
    <dbReference type="OMA" id="RFIYPGN"/>
    <dbReference type="OrthoDB" id="1105091at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030288">
        <property type="term" value="C:outer membrane-bounded periplasmic space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061077">
        <property type="term" value="P:chaperone-mediated protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008962">
        <property type="entry name" value="PapD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036316">
        <property type="entry name" value="Pili_assmbl_chap_C_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001829">
        <property type="entry name" value="Pili_assmbl_chaperone_bac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016148">
        <property type="entry name" value="Pili_assmbl_chaperone_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016147">
        <property type="entry name" value="Pili_assmbl_chaperone_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02753">
        <property type="entry name" value="PapD_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00345">
        <property type="entry name" value="PapD_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00969">
        <property type="entry name" value="CHAPERONPILI"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49354">
        <property type="entry name" value="SSF49354"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49584">
        <property type="entry name" value="SSF49584"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0143">Chaperone</keyword>
    <keyword evidence="3" id="KW-0574">Periplasm</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193766" evidence="5">
        <location>
            <begin position="26"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="domain" description="PapD_N" evidence="6">
        <location>
            <begin position="27"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="domain" description="PapD_C" evidence="7">
        <location>
            <begin position="177"/>
            <end position="239"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007399"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022764"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF02753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ABF07461.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="256" mass="27543" checksum="5DE315430233A803" modified="2006-05-30" version="1">MFKTSICRAAIAAVLLAMTAPGTLASVVLTGTRVIYPESEREVSLKLTNEGDAPSLVQAWIDDGDPNATPENAKSLFVLTPPLFRLDAKKGQTLRIIQLQQALSRDQESLFWLNVLEVPPIASSSKDPQNSLQFAFRSRIKLFFRPTGLPGDADSAPTKVTWRFVRKDDGHHVLTATNPTPYHITYTKVEATSGGHVYSNDAGAMVNPGGTVEFAIDDASPVSGEPAQVSYTFISDYGAGVSGVHNAKPTQEAKGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="110" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LR25</accession>
    <name>Q1LR25_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">DNA-binding response regulator in two-component regulatory system with EnvZ</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">ompR</name>
        <name evidence="6" type="ordered locus">Rmet_0515</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07401.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008645039.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0515"/>
    <dbReference type="EnsemblBacteria" id="ABF07401">
        <property type="protein sequence ID" value="ABF07401"/>
        <property type="gene ID" value="Rmet_0515"/>
    </dbReference>
    <dbReference type="GeneID" id="60824112"/>
    <dbReference type="KEGG" id="rme:Rmet_0515"/>
    <dbReference type="eggNOG" id="COG0745">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_30_4_4"/>
    <dbReference type="OMA" id="LMMSARG"/>
    <dbReference type="OrthoDB" id="817710at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00383">
        <property type="entry name" value="trans_reg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001867">
        <property type="entry name" value="OmpR/PhoB-type_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016032">
        <property type="entry name" value="Sig_transdc_resp-reg_C-effctor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039420">
        <property type="entry name" value="WalR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48111">
        <property type="entry name" value="PTHR48111"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00486">
        <property type="entry name" value="Trans_reg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00862">
        <property type="entry name" value="Trans_reg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46894">
        <property type="entry name" value="SSF46894"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51755">
        <property type="entry name" value="OMPR_PHOB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 3 6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Response regulatory" evidence="4">
        <location>
            <begin position="8"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="domain" description="OmpR/PhoB-type" evidence="5">
        <location>
            <begin position="136"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="OmpR/PhoB-type" evidence="3">
        <location>
            <begin position="136"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="2">
        <location>
            <position position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABF07401.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="244" mass="27694" checksum="97B642A7ADB02968" modified="2006-05-30" version="1">MRTNQPTQILVVDDDPELRDLLREYLTSQGFAVSVLHDGDGLQARLERERPALIVLDLMMPKVDGLTALRNLRAKNDDIPVILLTARSDEIDRIVGLEIGADDYLGKPFSPRELLARINAVLRRKLARPAAAPEDRESVVFGPFRVNFRQRSLERSGQSVSISDTEFALLKLLLSHPLEVLSRERIVELMYGTANGISDRGIDVQIWRLRRLLDEDAQRPRYIQTVRGRGYTFVPDEAEDSIPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LRJ7</accession>
    <name>Q1LRJ7_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Putative transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Rmet_0343</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07229.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008644433.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0343"/>
    <dbReference type="EnsemblBacteria" id="ABF07229">
        <property type="protein sequence ID" value="ABF07229"/>
        <property type="gene ID" value="Rmet_0343"/>
    </dbReference>
    <dbReference type="GeneID" id="60823945"/>
    <dbReference type="KEGG" id="rme:Rmet_0343"/>
    <dbReference type="eggNOG" id="COG1737">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_055769_1_3_4"/>
    <dbReference type="OMA" id="TWVLAFS"/>
    <dbReference type="OrthoDB" id="1540164at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097367">
        <property type="term" value="F:carbohydrate derivative binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006096">
        <property type="term" value="P:glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05013">
        <property type="entry name" value="SIS_RpiR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000281">
        <property type="entry name" value="HTH_RpiR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035472">
        <property type="entry name" value="RpiR-like_SIS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001347">
        <property type="entry name" value="SIS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046348">
        <property type="entry name" value="SIS_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01418">
        <property type="entry name" value="HTH_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01380">
        <property type="entry name" value="SIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53697">
        <property type="entry name" value="SSF53697"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51071">
        <property type="entry name" value="HTH_RPIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="1" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="HTH rpiR-type" evidence="3">
        <location>
            <begin position="13"/>
            <end position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF07229.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="298" mass="32132" checksum="842C638C88128128" modified="2006-05-30" version="1">MPASTPKPPQSLDALLAQLRADFPNLSAQFQSGARYLLDHPRDIAIQSMRKVAGNAGVQPATLVRLSQALGFEGWQGLRELFVEAVRDGDSQPYARRARKVVRESGSSRMFAEMIEVQHHNLDALLASNADALPRAAELLAAAPMVHVAGFRSCFPIAFSFHYIYGLFRSSVRLIRGDAGTLEMELRALSPKDAVLVAGFAPYSQESMRVATAAREAGCTVIALTDSTVAPIALEADCTLLFAIDSPSFFPSITSGVAMVEALVEHLLAKKGKGAIRALEQTEGQLHRTGAYLPSRRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="58" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LRN1</accession>
    <name>Q1LRN1_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">RHH_4 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_0309</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07195.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515195.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0309"/>
    <dbReference type="EnsemblBacteria" id="ABF07195">
        <property type="protein sequence ID" value="ABF07195"/>
        <property type="gene ID" value="Rmet_0309"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0309"/>
    <dbReference type="eggNOG" id="COG4321">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_155738_2_0_4"/>
    <dbReference type="OrthoDB" id="1837705at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3990.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027373">
        <property type="entry name" value="RHH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038268">
        <property type="entry name" value="RHH_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13467">
        <property type="entry name" value="RHH_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RHH_4" evidence="2">
        <location>
            <begin position="16"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="123"/>
            <end position="142"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF07195.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="142" mass="15783" checksum="24A030D135BC5DC2" modified="2006-05-30" version="1">MCEIFIRANPDSYQTQSRSLRLHGMATSIRLENLFWEVLEELAQRDGMTVNQLLTRLHDELAEHRGETSLSANFASFLRVCCMRYLLLQNDGRIPADTSVPIRSLDARAVLDSLPASWVEAGNSPLPRAARGEHASASSSSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LRT2</accession>
    <name>Q1LRT2_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative amidophosphoribosyltransferase</fullName>
            <ecNumber evidence="3">2.4.2.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">gntX</name>
        <name evidence="3" type="ordered locus">Rmet_0258</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.4.2.-" evidence="3"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07144.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0258"/>
    <dbReference type="EnsemblBacteria" id="ABF07144">
        <property type="protein sequence ID" value="ABF07144"/>
        <property type="gene ID" value="Rmet_0258"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0258"/>
    <dbReference type="eggNOG" id="COG1040">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_054549_0_1_4"/>
    <dbReference type="OMA" id="RYNQSAL"/>
    <dbReference type="OrthoDB" id="1937725at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06223">
        <property type="entry name" value="PRTases_typeI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2020">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000836">
        <property type="entry name" value="PRibTrfase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029057">
        <property type="entry name" value="PRTase-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00156">
        <property type="entry name" value="Pribosyltran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53271">
        <property type="entry name" value="SSF53271"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Pribosyltran" evidence="2">
        <location>
            <begin position="186"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF07144.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="279" mass="29726" checksum="2EAF7DE230E6699B" modified="2006-05-30" version="1">MQGHGETERNQWNRRAAQQRVAPRHGPDPDGRPTGTGVYAPSSQLAGRAGGITGLWSALLPSVCLICAKVQRDVVCAGCADTLLRIVPRCPRCAMPGTIPGVKAGRCDACADDDPIDATLTLGNYITPADRLVLRLKFAARLPAAAWIARQMATRMQAAGIAPDVLAPIPLAPQRLAGRGFNQAWEIARPLGRQLGIAVSATLLTRRRDTVSQRVLDQSARQANLRDAFVLDPPCRLDGLHIGLVDDVMTTGATLREAARVLKAHGAARVTALPALRTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="98" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LRV0</accession>
    <name>Q1LRV0_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Nitrogen regulatory protein PII-2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">glnK</name>
        <name evidence="5" type="ordered locus">Rmet_0240</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the P(II) protein family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07126.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008652254.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0240"/>
    <dbReference type="EnsemblBacteria" id="ABF07126">
        <property type="protein sequence ID" value="ABF07126"/>
        <property type="gene ID" value="Rmet_0240"/>
    </dbReference>
    <dbReference type="GeneID" id="60822965"/>
    <dbReference type="KEGG" id="rme:Rmet_0240"/>
    <dbReference type="eggNOG" id="COG0347">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_082268_0_0_4"/>
    <dbReference type="OMA" id="YRGTEHV"/>
    <dbReference type="OrthoDB" id="2021322at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030234">
        <property type="term" value="F:enzyme regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006808">
        <property type="term" value="P:regulation of nitrogen utilization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002187">
        <property type="entry name" value="N-reg_PII"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011322">
        <property type="entry name" value="N-reg_PII-like_a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015867">
        <property type="entry name" value="N-reg_PII/ATP_PRibTrfase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017918">
        <property type="entry name" value="N-reg_PII_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002332">
        <property type="entry name" value="N-reg_PII_urydylation_site"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30115">
        <property type="entry name" value="PTHR30115"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00543">
        <property type="entry name" value="P-II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF039144">
        <property type="entry name" value="GlnB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00340">
        <property type="entry name" value="PIIGLNB"/>
    </dbReference>
    <dbReference type="SMART" id="SM00938">
        <property type="entry name" value="P-II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54913">
        <property type="entry name" value="SSF54913"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00638">
        <property type="entry name" value="PII_GLNB_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51343">
        <property type="entry name" value="PII_GLNB_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00496">
        <property type="entry name" value="PII_GLNB_UMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="modified residue" description="O-UMP-tyrosine" evidence="2">
        <location>
            <position position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR039144-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR602187-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABF07126.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="112" mass="12177" checksum="66DFFFCC558C4810" modified="2006-05-30" version="1">MKLIIAVIKPFKLDEVREALSDVGVSGITVTEVKGFGRQKGHTELYRGAEYIVDFLPKVKIEVAVPDEVVERAIEAVEKSARTGKIGDGKIFVAPIEQVIRIRTGETGGDAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LS23</accession>
    <name>Q1LS23_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">4HBT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_0167</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07053.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008648494.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0167"/>
    <dbReference type="EnsemblBacteria" id="ABF07053">
        <property type="protein sequence ID" value="ABF07053"/>
        <property type="gene ID" value="Rmet_0167"/>
    </dbReference>
    <dbReference type="GeneID" id="60822890"/>
    <dbReference type="KEGG" id="rme:Rmet_0167"/>
    <dbReference type="eggNOG" id="COG2050">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_089876_3_2_4"/>
    <dbReference type="OMA" id="KPYFGID"/>
    <dbReference type="OrthoDB" id="1814691at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029069">
        <property type="entry name" value="HotDog_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003736">
        <property type="entry name" value="PAAI_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006683">
        <property type="entry name" value="Thioestr_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03061">
        <property type="entry name" value="4HBT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54637">
        <property type="entry name" value="SSF54637"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00369">
        <property type="entry name" value="unchar_dom_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="4HBT" evidence="1">
        <location>
            <begin position="54"/>
            <end position="129"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF07053.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="144" mass="15362" checksum="A2DE610C8AA53038" modified="2006-05-30" version="1">MNEIIPAAPGTPDKPYFGIDIPLMRLLGLQPVHMEEGLCRTRLPANPNVVNSRGDVHGGTLMAVLDFTLSGAARSHAPLETGVITIDMSTHFLSAARGELTFEARIMRRGAKIAFCEGEARDATGTLCCVARASFKLVKLVGGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LS60</accession>
    <name>Q1LS60_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Extra-cytoplasmic Solute Receptor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">bug</name>
        <name evidence="4" type="ordered locus">Rmet_0130</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Periplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the UPF0065 (bug) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07016.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515048.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0130"/>
    <dbReference type="EnsemblBacteria" id="ABF07016">
        <property type="protein sequence ID" value="ABF07016"/>
        <property type="gene ID" value="Rmet_0130"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0130"/>
    <dbReference type="eggNOG" id="COG3181">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045683_0_0_4"/>
    <dbReference type="OMA" id="WHAMYAP"/>
    <dbReference type="OrthoDB" id="988124at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042597">
        <property type="term" value="C:periplasmic space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005064">
        <property type="entry name" value="BUG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042100">
        <property type="entry name" value="Bug_dom1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42928">
        <property type="entry name" value="PTHR42928"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03401">
        <property type="entry name" value="TctC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017082">
        <property type="entry name" value="YflP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0675">Receptor</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193743" evidence="3">
        <location>
            <begin position="26"/>
            <end position="328"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF07016.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="328" mass="34355" checksum="D498F432D52D9CC6" modified="2006-05-30" version="1">MKRVALKFAAVLAVAGVGVFSGAQAQEYPAGKPVQAVVPFAAGGPTDKVARELTMVMSKHLGTTIVIENLGGAGGTIGAKKVAQSKGDGYTILIHHIGMSTAPALYRNLGFDPLKDFEMVGEIADVPMVLVGNKQLPPASFKELVPYIKANSSKLSLANAGIGSASHLCGLLFQSAIQTELTTVPYKGTAPALTDILGGQVNLMCDQTTNIAGQLKAGALKPYAAMQPRRVEAFKDIPTAAEQGLPGVEVKIWHAMYAPKGTPKPVIDKLSAALQKSVADPNFRAKMAELGAEAVPAQRATPDSLRVFLASEINKWTPVIKKAGVYAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="53" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LSG5</accession>
    <name>Q1LSG5_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_0025</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF06911.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011514958.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0025"/>
    <dbReference type="EnsemblBacteria" id="ABF06911">
        <property type="protein sequence ID" value="ABF06911"/>
        <property type="gene ID" value="Rmet_0025"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0025"/>
    <dbReference type="HOGENOM" id="CLU_165993_0_0_4"/>
    <dbReference type="OMA" id="PACERCL"/>
    <dbReference type="OrthoDB" id="1934881at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF06911.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="103" mass="11206" checksum="454D1FD54011826A" modified="2006-05-30" version="1">MVVMKSAVLQPPGTPAMPAAVPSRAPAHPACAHCLALADDPRRREPHPCLALHNTAPLVSQSAAGVPFSMLSFRCRECGTAWRLYDRANELFVSWVPEHPLVR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="127" xmlns="http://uniprot.org/uniprot">
    <accession>Q44004</accession>
    <accession>Q1LPC3</accession>
    <name>SBNA_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">N-(2-amino-2-carboxyethyl)-L-glutamate synthase</fullName>
            <shortName evidence="2">ACEGA synthase</shortName>
            <ecNumber evidence="1">2.5.1.140</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">sbnA</name>
        <name type="ordered locus">Rmet_1117</name>
    </gene>
    <organism>
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="J. Bacteriol." volume="178" first="5499" last="5507">
            <title>Siderophore-mediated iron uptake in Alcaligenes eutrophus CH34 and identification of aleB encoding the ferric iron-alcaligin E receptor.</title>
            <authorList>
                <person name="Gilis A."/>
                <person name="Khan M.A."/>
                <person name="Cornelis P."/>
                <person name="Meyer J.M."/>
                <person name="Mergeay M."/>
                <person name="van der Lelie D."/>
            </authorList>
            <dbReference type="PubMed" id="8808942"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC DNA]</scope>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the synthesis of N-((2S)-2-amino-2-carboxyethyl)-L-glutamate (ACEGA) from O-phospho-L-serine and L-glutamate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>L-glutamate + O-phospho-L-serine = H(+) + N-[(2S)-2-amino-2-carboxyethyl]-L-glutamate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:52384"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57524"/>
            <dbReference type="ChEBI" id="CHEBI:134610"/>
            <dbReference type="EC" id="2.5.1.140"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="1">Siderophore biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the cysteine synthase/cystathionine beta-synthase family. SbnA subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.140" evidence="1"/>
    <dbReference type="EMBL" id="X97499">
        <property type="protein sequence ID" value="CAA66130.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08003.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515904.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q44004"/>
    <dbReference type="SMR" id="Q44004"/>
    <dbReference type="STRING" id="266264.Rmet_1117"/>
    <dbReference type="EnsemblBacteria" id="ABF08003">
        <property type="protein sequence ID" value="ABF08003"/>
        <property type="gene ID" value="Rmet_1117"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1117"/>
    <dbReference type="eggNOG" id="COG0031">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021018_1_0_4"/>
    <dbReference type="OMA" id="GSWCPDQ"/>
    <dbReference type="OrthoDB" id="1677960at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016765">
        <property type="term" value="F:transferase activity, transferring alkyl or aryl (other than methyl) groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006535">
        <property type="term" value="P:cysteine biosynthetic process from serine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1100">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001216">
        <property type="entry name" value="P-phosphate_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001926">
        <property type="entry name" value="PLP-dep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023927">
        <property type="entry name" value="SbnA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036052">
        <property type="entry name" value="Trypto_synt_PLP_dependent"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00291">
        <property type="entry name" value="PALP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53686">
        <property type="entry name" value="SSF53686"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03945">
        <property type="entry name" value="PLP_SbnA_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00901">
        <property type="entry name" value="CYS_SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000167105" description="N-(2-amino-2-carboxyethyl)-L-glutamate synthase">
        <location>
            <begin position="1"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="73"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="181"/>
            <end position="185"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="268"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="1">
        <location>
            <position position="43"/>
        </location>
    </feature>
    <feature type="sequence conflict" description="In Ref. 1; CAA66130." evidence="2" ref="1">
        <original>QL</original>
        <variation>HV</variation>
        <location>
            <begin position="17"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="sequence conflict" description="In Ref. 1; CAA66130." evidence="2" ref="1">
        <original>QL</original>
        <variation>HV</variation>
        <location>
            <begin position="168"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="sequence conflict" description="In Ref. 1; CAA66130." evidence="2" ref="1">
        <original>S</original>
        <variation>T</variation>
        <location>
            <position position="181"/>
        </location>
    </feature>
    <feature type="sequence conflict" description="In Ref. 1; CAA66130." evidence="2" ref="1">
        <original>GSVLFGQPSQARELPGIGASRVPELLCRDEIDEVIHIDDYTAATACRRLLAREGIFA</original>
        <variation>FPCCSANRRKRASCRALAPRRAGIAVPGRDRRSDPYRRLHRRHGAGACWRRRHLR</variation>
        <location>
            <begin position="209"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="sequence conflict" description="In Ref. 1; CAA66130." evidence="2" ref="1">
        <original>R</original>
        <variation>T</variation>
        <location>
            <position position="320"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1">
        <source>
            <dbReference type="UniProtKB" id="A6QDA0"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="341" mass="36838" checksum="46EE65F2BBCD5F88" modified="2006-07-11" version="2">MVCNSIIESIGNTPLVQLRRLFGRADAEVFAKLELLNPAGSVKDRPARYIVEKGLTEGAIGPHSHVIESSSGNLAIALAMICGLKGLRFTAVVDPKISPTNLKILRCYGAGIEQVTAKDSQGGYLETRIERVRQMLREQRDAIWINQYANPLNWESHYYGEAAEILAQLAQPADVLVLGVSTSGTVHGIARRLRKAWPSMRVVGVDAVGSVLFGQPSQARELPGIGASRVPELLCRDEIDEVIHIDDYTAATACRRLLAREGIFAGGSSGAVVAAIEQVLHATPVSHRPLRILTLFPDRGERYLDTVYDDAWLARIAARRAVPNLAALPTVASLIPAGVSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-04-26" modified="2021-06-02" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>Q58AP7</accession>
    <name>Q58AP7_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Putative cysteine peptidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_6009</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="1 2">
        <name>pMOL30</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid pMOL30</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000354">
        <property type="protein sequence ID" value="ABF12868.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011229322.1">
        <property type="nucleotide sequence ID" value="NC_007971.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_145568.1">
        <property type="nucleotide sequence ID" value="NC_006466.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF12868">
        <property type="protein sequence ID" value="ABF12868"/>
        <property type="gene ID" value="Rmet_6009"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6009"/>
    <dbReference type="HOGENOM" id="CLU_1032999_0_0_4"/>
    <dbReference type="OMA" id="STSYAHC"/>
    <dbReference type="OrthoDB" id="1435175at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid pMOL30"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF12868.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="279" mass="31504" checksum="1CDCBF65F15C3415" modified="2005-04-26" version="1">MSHFSKAVDRRSRRAMTEFLSSHFRYHTAGSHNRSTSYAHCIKVDRLGLNREQTDAAYDMLAAETYWDRIDDPIREFTHSMHGMYTIGTNGRSGGYLVLYRSEYKSTEHLSFCRSCGQRNFRRVAPTLDGAEGIIGAEILRNGGLWHDDVYLGQSAIQAIALSDTEKLAMVSKLKPLLKDCSADNRCGVCSATGDRGRVNYEKSPVTLSHWPMRPIDQDADFSDMSMDELRSRVDVVTAFDAACDEIRENFIELLSDFAPVERTIMVPKTVTVLERRAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-01-04" modified="2021-06-02" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>Q5NV15</accession>
    <name>Q5NV15_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_6249</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="1 2">
        <name>pMOL28</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid pMOL28</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000355">
        <property type="protein sequence ID" value="ABF13108.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011239936.1">
        <property type="nucleotide sequence ID" value="NC_007972.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_161675.1">
        <property type="nucleotide sequence ID" value="NC_006525.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF13108">
        <property type="protein sequence ID" value="ABF13108"/>
        <property type="gene ID" value="Rmet_6249"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6249"/>
    <dbReference type="HOGENOM" id="CLU_055770_0_0_4"/>
    <dbReference type="OMA" id="WGVCNAR"/>
    <dbReference type="OrthoDB" id="369463at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid pMOL28"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009553">
        <property type="entry name" value="DUF1173"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06666">
        <property type="entry name" value="DUF1173"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF13108.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="408" mass="44858" checksum="C65E169E5ECCE0D6" modified="2005-01-04" version="1">MITVRIGGNEFPLDDVLDDPGRYARHLERAKKIQGFAECGCGTQRPRPKLVIRRHRDIFLLARWPEQAHQHAAACPFNRPTLAKSGPTDNLDAFRLKDGHHDIRLGVALSVSTRTPAATVQRSAQGQSSKPQRRSAGLLAFLEYAWEQAGLNVWPGTGYRGWIACWSQLTAELAGCRINGTSADGLLHIVQRWDPSRKAEILAEFDAWQARLTPMAAGNPRGIVIGQLESHEPSQYGGKLVLRQSRQRYFLNADLYACLQSSFGSALSAVGRDDQRCVAILLVEMSKGGYLRVVDIAAMLTNSHFVPCDSSHEIAMADHLIAERRAFRKPLRHIGRAAAHPDFILTDVAPEVVIEVLGMSGNADYDARIAEKRAHYLASGIPLLEWDVKAGPAASVRLPTANRSNDGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2001-03-01" modified="2022-08-03" version="115" xmlns="http://uniprot.org/uniprot">
    <accession>Q9F8S6</accession>
    <name>Q9F8S6_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Transposase</fullName>
        </submittedName>
        <submittedName>
            <fullName evidence="9">Transposase ISRme1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">tnpA</name>
        <name evidence="9" type="ordered locus">Rmet_1171</name>
        <name evidence="10" type="ordered locus">Rmet_3353</name>
        <name evidence="11" type="ordered locus">Rmet_4153</name>
        <name evidence="12" type="ordered locus">Rmet_4228</name>
    </gene>
    <organism evidence="8">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="11">
        <name>megaplasmid</name>
    </geneLocation>
    <geneLocation type="plasmid" evidence="13">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2000" name="Plasmid" volume="44" first="201" last="207">
            <title>A broad-host-range plasmid for isolating mobile genetic elements in gram-negative bacteria.</title>
            <authorList>
                <person name="Schneider D."/>
                <person name="Faure D."/>
                <person name="Noirclerc-Savoye M."/>
                <person name="Barriere A.C."/>
                <person name="Coursange E."/>
                <person name="Blot M."/>
            </authorList>
            <dbReference type="PubMed" id="10964631"/>
            <dbReference type="DOI" id="10.1006/plas.2000.1483"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">CH34</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2000-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Faure D.D."/>
                <person name="Schneider D.D."/>
                <person name="Blot M.M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">CH34</strain>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2006-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of the chromosome of Ralstonia metallidurans CH34.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Copeland A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Barry K."/>
                <person name="Detter J.C."/>
                <person name="Glavina del Rio T."/>
                <person name="Hammon N."/>
                <person name="Israni S."/>
                <person name="Dalin E."/>
                <person name="Tice H."/>
                <person name="Martinez M."/>
                <person name="Goltsman E."/>
                <person name="Pitluck S."/>
                <person name="Schmutz J."/>
                <person name="Larimer F."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Kim E."/>
                <person name="Mergeay M."/>
                <person name="Benotmane M.A."/>
                <person name="Vallaeys T."/>
                <person name="Michaux A."/>
                <person name="Monchy S."/>
                <person name="Dunn J."/>
                <person name="McCorkle S."/>
                <person name="Taghavi S."/>
                <person name="van der Lelie D."/>
                <person name="Richardson P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">CH34</strain>
            <plasmid evidence="11">megaplasmid</plasmid>
        </source>
    </reference>
    <reference evidence="13" key="4">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="13">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <reference evidence="9" key="5">
        <citation type="submission" date="2010-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Benotmane R."/>
                <person name="Lapidus A."/>
                <person name="McCorkle S."/>
                <person name="Monchy S."/>
                <person name="Taghavi S."/>
                <person name="van der Lelie N."/>
                <person name="Dunn J."/>
                <person name="Leys N."/>
                <person name="Mergeay M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">CH34</strain>
            <plasmid evidence="11">megaplasmid</plasmid>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in the transposition of the insertion sequence IS5.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the transposase 11 family.</text>
    </comment>
    <dbReference type="EMBL" id="AF236817">
        <property type="protein sequence ID" value="AAG18510.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08057.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10225.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11020.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11095.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515952.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1171"/>
    <dbReference type="EnsemblBacteria" id="ABF08057">
        <property type="protein sequence ID" value="ABF08057"/>
        <property type="gene ID" value="Rmet_1171"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF10225">
        <property type="protein sequence ID" value="ABF10225"/>
        <property type="gene ID" value="Rmet_3353"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF11020">
        <property type="protein sequence ID" value="ABF11020"/>
        <property type="gene ID" value="Rmet_4153"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF11095">
        <property type="protein sequence ID" value="ABF11095"/>
        <property type="gene ID" value="Rmet_4228"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1171"/>
    <dbReference type="KEGG" id="rme:Rmet_3353"/>
    <dbReference type="KEGG" id="rme:Rmet_4153"/>
    <dbReference type="KEGG" id="rme:Rmet_4228"/>
    <dbReference type="eggNOG" id="COG3039">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021293_3_2_4"/>
    <dbReference type="OMA" id="WIKTVGG"/>
    <dbReference type="OrthoDB" id="568762at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004803">
        <property type="term" value="F:transposase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006313">
        <property type="term" value="P:transposition, DNA-mediated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002559">
        <property type="entry name" value="Transposase_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008490">
        <property type="entry name" value="Transposase_InsH_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01609">
        <property type="entry name" value="DDE_Tnp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05598">
        <property type="entry name" value="DUF772"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0614">Plasmid</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0814">Transposable element</keyword>
    <keyword evidence="4" id="KW-0815">Transposition</keyword>
    <feature type="domain" description="DUF772" evidence="7">
        <location>
            <begin position="56"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="domain" description="DDE_Tnp_1" evidence="6">
        <location>
            <begin position="160"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="164"/>
            <end position="197"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022578"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF05598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="AAG18510.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ABF08057.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ABF10225.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ABF11020.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ABF11095.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="366" mass="41472" checksum="34ABE4B870FF92BE" modified="2001-03-01" version="1">MRGADTFTESLFTMRRLEDFVPKSHPLRAIRTMANQALAKMDRLFAQMYEADIKGGRPSIAPEKLLRAMLLQVLYSVRSERQLMEQTHYNLLFRWFIGLAMDDVVWVPSVFSKNRERLIKHDAVIDFFNEVLAIAQKKDWLSGEHFSVDGTLIKAWAGHKSFVRKDGDDQDDNDGADFKGEKRSNETHESKTDPDAKLYRKGKTASELRYMGHTLSDNRHGLVVSAMVTNADGHAEREAAKVMLNDARQVIEDLNVEVTVGADKGYDAHEFIQACLELKVTPHVAQNTSGRRSAVPDAIAGTKGYAISQQKRKLIEQGFGWAKTVGRMRQVMVRGLKKVDQMFVLSMAAYNLVRMRSLGQIRPQLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-03-23" modified="2020-10-07" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>D3DXX9</accession>
    <name>D3DXX9_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_6542</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ADC45149.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADC45149">
        <property type="protein sequence ID" value="ADC45149"/>
        <property type="gene ID" value="Rmet_6542"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6542"/>
    <dbReference type="HOGENOM" id="CLU_3423058_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADC45149.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="23" mass="2541" checksum="214A2DC43E6EEA8D" modified="2010-03-23" version="1">MTGASPGGYRIIVVQMQRATWDC</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-03-23" modified="2021-02-10" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>D3DYI4</accession>
    <name>D3DYI4_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_6368</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3 4">
        <name>pMOL30</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid pMOL30</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000354">
        <property type="protein sequence ID" value="ADC45354.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004635335.1">
        <property type="nucleotide sequence ID" value="NC_007971.2"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADC45354">
        <property type="protein sequence ID" value="ADC45354"/>
        <property type="gene ID" value="Rmet_6368"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6368"/>
    <dbReference type="HOGENOM" id="CLU_2957398_0_0_4"/>
    <dbReference type="OrthoDB" id="2003836at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid pMOL30"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="15"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="38"/>
            <end position="59"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADC45354.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="59" mass="6889" checksum="F3A39C6B2963F3E7" modified="2010-03-23" version="1">MHYAHVSDSVVESMLVMLVGLIVLYVGFAVYLRWKHGPAPKRKTDEGRKRHPKTSRRKR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1994-10-01" modified="2022-05-25" version="118" xmlns="http://uniprot.org/uniprot">
    <accession>P37973</accession>
    <accession>Q5NUX4</accession>
    <accession>Q7B055</accession>
    <name>CNRB_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName>Nickel and cobalt resistance protein CnrB</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">cnrB</name>
        <name type="ordered locus">Rmet_6209</name>
        <name type="ORF">RMe0084</name>
    </gene>
    <organism>
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid">
        <name>pMOL28</name>
    </geneLocation>
    <reference key="1">
        <citation type="journal article" date="1993" name="J. Bacteriol." volume="175" first="767" last="778">
            <title>Characterization of the inducible nickel and cobalt resistance determinant cnr from pMOL28 of Alcaligenes eutrophus CH34.</title>
            <authorList>
                <person name="Liesegang H."/>
                <person name="Lemke K."/>
                <person name="Siddiqui R.A."/>
                <person name="Schlegel H.G."/>
            </authorList>
            <dbReference type="PubMed" id="8380802"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC DNA]</scope>
    </reference>
    <reference key="2">
        <citation type="submission" date="2004-10" db="EMBL/GenBank/DDBJ databases">
            <title>Sequence and features of the Ralstonia metallidurans CH34 heavy metal plasmids pMOL28 and pMOL30.</title>
            <authorList>
                <person name="van der Lelie D."/>
                <person name="Monchy S."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="Benotmane M."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Mergeay M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference key="3">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <reference key="4">
        <citation type="journal article" date="2000" name="J. Bacteriol." volume="182" first="1390" last="1398">
            <title>Regulation of the cnr cobalt and nickel resistance determinant from Ralstonia sp. strain CH34.</title>
            <authorList>
                <person name="Grass G."/>
                <person name="Grosse C."/>
                <person name="Nies D.H."/>
            </authorList>
            <dbReference type="PubMed" id="10671463"/>
            <dbReference type="DOI" id="10.1128/JB.182.5.1390-1398.2000"/>
        </citation>
        <scope>INDUCTION</scope>
    </reference>
    <comment type="function">
        <text>The products of the genes cnrA, cnrB, and cnrC are likely to form a membrane-bound protein complex catalyzing an energy-dependent efflux of Ni(2+) and Co(2+). The mechanism of action of the CnrCBA complex may be that of a proton/cation antiporter.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cell inner membrane</location>
            <topology evidence="4">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="induction">
        <text evidence="3">By nickel.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family.</text>
    </comment>
    <dbReference type="EMBL" id="M91650">
        <property type="protein sequence ID" value="AAA21969.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AJ276513">
        <property type="protein sequence ID" value="CAB82452.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="X90708">
        <property type="protein sequence ID" value="CAI30228.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP000355">
        <property type="protein sequence ID" value="ABF13068.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F47056">
        <property type="entry name" value="F47056"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011239967.1">
        <property type="nucleotide sequence ID" value="NC_007972.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_161706.1">
        <property type="nucleotide sequence ID" value="NC_006525.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="P37973"/>
    <dbReference type="SMR" id="P37973"/>
    <dbReference type="TCDB" id="8.A.1.2.1">
        <property type="family name" value="the membrane fusion protein (mfp) family"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF13068">
        <property type="protein sequence ID" value="ABF13068"/>
        <property type="gene ID" value="Rmet_6209"/>
    </dbReference>
    <dbReference type="GeneID" id="60825773"/>
    <dbReference type="KEGG" id="rme:Rmet_6209"/>
    <dbReference type="HOGENOM" id="CLU_018816_13_0_4"/>
    <dbReference type="OMA" id="AGDYCRE"/>
    <dbReference type="OrthoDB" id="1174564at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid pMOL28"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046873">
        <property type="term" value="F:metal ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005695">
        <property type="entry name" value="Co/Zn/Cd_resistance_CzcB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032317">
        <property type="entry name" value="HlyD_D23"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006143">
        <property type="entry name" value="RND_pump_MFP"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16576">
        <property type="entry name" value="HlyD_D23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00999">
        <property type="entry name" value="8a0102"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01730">
        <property type="entry name" value="RND_mfp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword id="KW-0997">Cell inner membrane</keyword>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0170">Cobalt</keyword>
    <keyword id="KW-0175">Coiled coil</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-0533">Nickel</keyword>
    <keyword id="KW-0614">Plasmid</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <keyword id="KW-0813">Transport</keyword>
    <feature type="chain" id="PRO_0000201863" description="Nickel and cobalt resistance protein CnrB">
        <location>
            <begin position="1"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="13"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="35"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="140"/>
            <end position="193"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="3">
        <source>
            <dbReference type="PubMed" id="10671463"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="4"/>
    <sequence length="395" mass="40083" checksum="075C2165EB5BA09F" modified="1994-10-01" version="1">MMKNERRSVNWPMIAGVAAVAAAVGFGAAHLPVSEKSPASTQAPEAQKPQSAPVKPGLKEVKIPATYLAAANIAVEPVASAAVGTEILAPATVAALPGSEAVIVSRAAGAVQRVQRRLGDVVKAGDVLALVDSPEAAGMAAERKVAQAKADLARKTYEREASLFQQGVTPRQEMEAAKAALDVAQAEALRAATVAQSAHLASDGRSVAVVSPIAGKITAQSVTLGAFVAPQAELFRVAGTGAVQVEAAVTAADTSRIVAGSEATILLANGSPLSARVQAVTPTVTGSARVATVVVVPAQPTDRLVVGEGVQVRLRTAVADAAALSVPEDAVQNLDGRDVLFVRTQEGFRPMPVLVGTRSGGSAQILSGVQAGEQVATRNAFLVKAEMNKGGGDEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="65" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LAP3</accession>
    <name>Q1LAP3_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Protein SlyX homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">slyX</name>
        <name evidence="3" type="ordered locus">Rmet_5924</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SlyX family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12783.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008645462.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5924"/>
    <dbReference type="EnsemblBacteria" id="ABF12783">
        <property type="protein sequence ID" value="ABF12783"/>
        <property type="gene ID" value="Rmet_5924"/>
    </dbReference>
    <dbReference type="GeneID" id="60823721"/>
    <dbReference type="KEGG" id="rme:Rmet_5924"/>
    <dbReference type="eggNOG" id="COG2900">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_180796_3_1_4"/>
    <dbReference type="OMA" id="LNMTIYR"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00715">
        <property type="entry name" value="SlyX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007236">
        <property type="entry name" value="SlyX"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36508">
        <property type="entry name" value="PTHR36508"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04102">
        <property type="entry name" value="SlyX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="19"/>
            <end position="46"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00715"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF12783.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="67" mass="7706" checksum="18D7ABB5DDD03567" modified="2006-05-30" version="1">MESRLTDLEIKVAFQEDLIETLNLAVARQQQQIDLLQAQFRALYQQVRASAPMAAESDPQHEIPPHY</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="104" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LB04</accession>
    <name>Q1LB04_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Sigma54 specific transcriptional regulator, Fis family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Rmet_5813</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="8">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="8">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12672.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5813"/>
    <dbReference type="EnsemblBacteria" id="ABF12672">
        <property type="protein sequence ID" value="ABF12672"/>
        <property type="gene ID" value="Rmet_5813"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5813"/>
    <dbReference type="eggNOG" id="COG2204">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_0_6_4"/>
    <dbReference type="OMA" id="RQYCFDY"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002197">
        <property type="entry name" value="HTH_Fis"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002078">
        <property type="entry name" value="Sigma_54_int"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025943">
        <property type="entry name" value="Sigma_54_int_dom_ATP-bd_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025944">
        <property type="entry name" value="Sigma_54_int_dom_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045343">
        <property type="entry name" value="VpsR"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02954">
        <property type="entry name" value="HTH_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00158">
        <property type="entry name" value="Sigma54_activat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20161">
        <property type="entry name" value="VpsR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00676">
        <property type="entry name" value="SIGMA54_INTERACT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00688">
        <property type="entry name" value="SIGMA54_INTERACT_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50045">
        <property type="entry name" value="SIGMA54_INTERACT_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0614">Plasmid</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Sigma-54 factor interaction" evidence="6">
        <location>
            <begin position="189"/>
            <end position="418"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50045"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABF12672.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="500" mass="55633" checksum="3A6ECE7418491025" modified="2006-05-30" version="1">MIPTLRAAPTAQPADPERAMRLAATPAGLGSATNKKFNVRENVMDRLCKAPTRTAQIVLVSRYEDFGFNANQFGSGWEIRRVQQVRDLERLVRASSPMAGVLDLQTPYTDAELSQLEQALQHLHVMWVAITSTAMLEDERIRRLIRDYCVDYVRTPFSFDELLYTLKHAHGMACLHGARSPELVARGPMIGECAPMGNMFRSLAKVAHNDAPVFISGESGTGKELAAQAIHEASSRRKGPFVAINCGAIPSHLVQSELFGYEKGAFTGANQRKIGWIEQAQGGTLFLDEIGDLPLESQVALLRFLQQGMITRLGGHQSIPLDLRIISATHVDLVAAQSDGRFRSDLFHRLCVLTLTIPPLRERGEDILLLANTVLAEHGHEAHRRIRGFSTCATQAMMQYMWPGNVRELINRVRRAIVMTDNRKITAEDLQLHGGVEVPRKTLDAIREEAEREAIRSVMASHGFHVVPAARELNVSRVTLYRLMHKHNIRIDTQPGASAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-10-07" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LBA8</accession>
    <name>Q1LBA8_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_5709</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12568.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF12568">
        <property type="protein sequence ID" value="ABF12568"/>
        <property type="gene ID" value="Rmet_5709"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5709"/>
    <dbReference type="HOGENOM" id="CLU_2495636_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF12568.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="86" mass="9242" checksum="34BFEA6F72C50A8F" modified="2006-05-30" version="1">MESSECKKMTHSVSRNAAASWLSETSLSESRCGLQALGAGLWRAVTRVRRAGSEVRIIDSRRPASDCVAMRAGAMPPCLDASGRFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LBV2</accession>
    <name>Q1LBV2_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Aldehyde dehydrogenase</fullName>
            <ecNumber evidence="6">1.2.1.3</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Rmet_5515</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="7">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="7">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the aldehyde dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.2.1.3" evidence="6"/>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12374.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519918.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5515"/>
    <dbReference type="EnsemblBacteria" id="ABF12374">
        <property type="protein sequence ID" value="ABF12374"/>
        <property type="gene ID" value="Rmet_5515"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5515"/>
    <dbReference type="eggNOG" id="COG1012">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005391_0_0_4"/>
    <dbReference type="OMA" id="QAYGQIA"/>
    <dbReference type="OrthoDB" id="744602at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004029">
        <property type="term" value="F:aldehyde dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043878">
        <property type="term" value="F:glyceraldehyde-3-phosphate dehydrogenase (NAD+) (non-phosphorylating) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd07106">
        <property type="entry name" value="ALDH_AldA-AAD23400"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.309.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.605.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016161">
        <property type="entry name" value="Ald_DH/histidinol_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016163">
        <property type="entry name" value="Ald_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016160">
        <property type="entry name" value="Ald_DH_CS_CYS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029510">
        <property type="entry name" value="Ald_DH_CS_GLU"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016162">
        <property type="entry name" value="Ald_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015590">
        <property type="entry name" value="Aldehyde_DH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044086">
        <property type="entry name" value="FUS7/LUC3-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00171">
        <property type="entry name" value="Aldedh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53720">
        <property type="entry name" value="SSF53720"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00070">
        <property type="entry name" value="ALDEHYDE_DEHYDR_CYS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00687">
        <property type="entry name" value="ALDEHYDE_DEHYDR_GLU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 4 6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-0614">Plasmid</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aldedh" evidence="5">
        <location>
            <begin position="26"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="active site" evidence="2">
        <location>
            <position position="250"/>
        </location>
    </feature>
    <feature type="active site" evidence="3">
        <location>
            <position position="284"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABF12374.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="479" mass="51704" checksum="DD7562068D4B3EF0" modified="2006-05-30" version="1">MTTLQGTPVASSFPMTIDGTSIDTPSTIEVVNPATGEVFAQAPDCNAEMLDAAVSAARNALPSWAKTTWAERQELLGRIGSVYLAHQEELARLLTAEQGKPLSRARTEVAGAAYWFAEFARMTLPDLIVQDNDQAYIVVRRVPVGVVGAMVPWNYPVILAAWKIAPALLTGNTLVLKPSPFTPLVTLRVGELMRDVLPPGVLNIVSGGDSLGPRMSAHPGFDKLSFTGSTVTGKRVMESASKDLKRLTLELGGNDAAIVMPDVDIEEVATKLFWGAFVNSGQICIAAKRVFIHESIYDKFSEVLVRLAKSTPMGPGDRDGVELGPVQNRAQFDRLCGLLQDCKDRGFNLLTGGEVEKGPGLFFPVTLVDNPSDDSRIVREEPFGPILPLLKFRSIPEVIERANASEYGLAGSVWCRDESIAMDIARRLQTGTVWINQIQAVTPHTPMAGHKQSGLGVENGEEGLASYTLPQTLSVKRAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LC21</accession>
    <name>Q1LC21_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Putative transporter major facilitator superfamily (MFS)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Rmet_5446</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="8">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="8">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12305.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519852.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5446"/>
    <dbReference type="EnsemblBacteria" id="ABF12305">
        <property type="protein sequence ID" value="ABF12305"/>
        <property type="gene ID" value="Rmet_5446"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5446"/>
    <dbReference type="eggNOG" id="COG2814">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001265_46_6_4"/>
    <dbReference type="OMA" id="IWRYSVA"/>
    <dbReference type="OrthoDB" id="1121832at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="SSF103473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0614">Plasmid</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="30"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="65"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="97"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="127"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="160"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="185"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="271"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="303"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="336"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="360"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="401"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="426"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="domain" description="MFS" evidence="6">
        <location>
            <begin position="31"/>
            <end position="452"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABF12305.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="484" mass="52232" checksum="DFA60B8AE0FB60EE" modified="2006-05-30" version="1">MQTSAIKIRGIDDVIAFIDSCPGIRGRAGVIWWLALGGLFLDAFSNSALSAGLGPMTRDLKLEPAQVALLTSFASWVAIAFNPLGGWMADRWGRVKPLILAKVFAVVGALLVVFAPDFNAILVGRFFVGAAYGIDFAIAMAVLAEFTPVKLKSRLNTWQGMWYTAVCLNLLLALLFYSWQVGDTIWRYSVAATAVFGVGILILQVLFLVESPTWLARKERLEAAAQAMTRIYGRPFEAAPLAERVPVTNMARRGLANVLLIFRGIYLPRTILAATVQIGQSIQYFGIGWYLPLISAALFGKDFVYATIGALVFNVCGIFGGFLSPVIGRKLGLRRASAYGFAAVFVMLLILGLFNGKMPTWVAVVVPSLFILFHSGGPGANGKSLSSLSFRSELRAGANGVIGALGAIGAALGLLVFPLFRARYGLETTFLILSVVPLVASIICFLIRWDPTRTAIHPDNEADAPQFRSDARDGAGFMHPATEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="60" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LC81</accession>
    <name>Q1LC81_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_5386</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12245.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519791.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF12245">
        <property type="protein sequence ID" value="ABF12245"/>
        <property type="gene ID" value="Rmet_5386"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5386"/>
    <dbReference type="eggNOG" id="ENOG50336RP">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_125984_0_0_4"/>
    <dbReference type="OMA" id="HESINCR"/>
    <dbReference type="OrthoDB" id="1804447at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193297" evidence="1">
        <location>
            <begin position="21"/>
            <end position="184"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF12245.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="184" mass="19605" checksum="3DF36CEF37A2F440" modified="2006-05-30" version="1">MKQCKFALVAAICATGVLLTGCETTNSIPYKASASNVIAIQQSLQSKKVSVSDVGMAPGVDESPLCRLMGPVKVAPGKTLSQYIKDAFQEELLMAQAYDVKGAPIQGRIEELSFSSVSPAYWQITMAVQSPADKGYKVSVKYPFDTSFTAMGACKNVADAFGPAVQELLKQVVTNPQFPKLSGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LCA4</accession>
    <name>Q1LCA4_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">N-ethylmaleimide reductase, FMN-linked</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">nemA</name>
        <name evidence="2" type="ordered locus">Rmet_5363</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12222.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5363"/>
    <dbReference type="EnsemblBacteria" id="ABF12222">
        <property type="protein sequence ID" value="ABF12222"/>
        <property type="gene ID" value="Rmet_5363"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5363"/>
    <dbReference type="eggNOG" id="COG1902">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012153_0_2_4"/>
    <dbReference type="OMA" id="MIGFGRP"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001155">
        <property type="entry name" value="OxRdtase_FMN_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045247">
        <property type="entry name" value="Oye-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22893">
        <property type="entry name" value="PTHR22893"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00724">
        <property type="entry name" value="Oxidored_FMN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Oxidored_FMN" evidence="1">
        <location>
            <begin position="9"/>
            <end position="346"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00724"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF12222.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="373" mass="39934" checksum="75BB26A169BC10A6" modified="2006-05-30" version="1">MMTSTSNPLFQPYSLGPLSLPNRIVMPPMTRSRASQPGDVPNALMAEYYAQRASAGLIVSEGTWISPLGRGYAWTPGIHTPEQVAGWRKVTTAVHQAGGRIFAQLWHVGRLSHISLLDGHAPVSSSAIQAQGVNVFVAQSDGKPGFAQASQPRALSVEEIHAIVDDFRQAARNAMAAGFDGVELHAANGYLVNQFIDSGANDRTDEYGGSLENRLRFLGEVAQALIEGTGGKDRVGIRLAPLTTLNGCVDADPETTYTAAARLLGEIGVGYIHIAEADWEDAPHMPTAFKQQLRKVYPGTLIYAGKYTAARAQAALHEGWADLIAFGRPFVANPDLPERLRTGATLNAHDRDTLFGGDARGLTDYPPLVAKAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LCD9</accession>
    <name>Q1LCD9_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Heavy metal cation tricomponent efflux outer membrane porin ZneC (CzcC-like)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">zneC</name>
        <name evidence="4" type="ordered locus">Rmet_5328</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="5">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="5">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the outer membrane factor (OMF) (TC 1.B.17) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12187.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519730.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5328"/>
    <dbReference type="TCDB" id="2.A.6.1.6">
        <property type="family name" value="the resistance-nodulation-cell division (rnd) superfamily"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF12187">
        <property type="protein sequence ID" value="ABF12187"/>
        <property type="gene ID" value="Rmet_5328"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5328"/>
    <dbReference type="eggNOG" id="COG1538">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012817_13_0_4"/>
    <dbReference type="OMA" id="VEPPRHE"/>
    <dbReference type="OrthoDB" id="1619477at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015562">
        <property type="term" value="F:efflux transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003423">
        <property type="entry name" value="OMP_efflux"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010131">
        <property type="entry name" value="RND_efflux_OM_lipoprot_NodT"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02321">
        <property type="entry name" value="OEP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01845">
        <property type="entry name" value="outer_NodT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0564">Palmitate</keyword>
    <keyword evidence="4" id="KW-0614">Plasmid</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1134">Transmembrane beta strand</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5001442006" evidence="2">
        <location>
            <begin position="22"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="478"/>
            <end position="511"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU362097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF12187.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="511" mass="53838" checksum="3E2D848E49B07590" modified="2006-05-30" version="1">MAKITSISVATLLALATAGCAVGPDFQRPAAPETDRYTATTLPAETASASTPDGQAQRFAPGQDVPAQWWTMFGSSELNALVDAALRANPDLQAMEAALRVAQENVAAQRGAFFPSVDASYTPSRQKIAQIIASPLSDNSDLFTLHTAQLSVSYVPDVFGGTRRQVESGVAQADVARFQWQAAYLTLTSNVVNAAIQEASLRAQIRATQDVIALSTRQLEAVRKQQRLGQVGAAEVAAQEATLAQAEAALPPLDRQLAQQRNLLAALTGRLPSDAVPQQFEFEALTLPGTLPLSLPSRLVGQRPDIRAAEAQMHAASAQIGVATAARLPNITLTAALGSSSQAIGDLFTAGTGFWGISAGLMQPIFKGGMLLHQQRAAEAAYKQASAQYRSTVLTAYQGVADALHAIEADARGLRTASDAERAAYRSFNIAQAQWKAGQIGYPAVMLAEQTYRQSAITLVQARASRYSDTVGLMQALGGSWEDEPGKTPENQSDSKPAAAPEAVGKAQGNG</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LCF4</accession>
    <name>Q1LCF4_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Amidohydro_3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_5313</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12172.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519717.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5313"/>
    <dbReference type="EnsemblBacteria" id="ABF12172">
        <property type="protein sequence ID" value="ABF12172"/>
        <property type="gene ID" value="Rmet_5313"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5313"/>
    <dbReference type="eggNOG" id="COG1574">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009942_4_3_4"/>
    <dbReference type="OMA" id="RDHHGAW"/>
    <dbReference type="OrthoDB" id="751534at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016810">
        <property type="term" value="F:hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01300">
        <property type="entry name" value="YtcJ_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013108">
        <property type="entry name" value="Amidohydro_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011059">
        <property type="entry name" value="Metal-dep_hydrolase_composite"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033932">
        <property type="entry name" value="YtcJ-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07969">
        <property type="entry name" value="Amidohydro_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51338">
        <property type="entry name" value="SSF51338"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="SSF51556"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Amidohydro_3" evidence="1">
        <location>
            <begin position="53"/>
            <end position="536"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF07969"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF12172.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="621" mass="68284" checksum="D92E5FCF837A8D06" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="95" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LCQ4</accession>
    <name>Q1LCQ4_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Transcriptional regulator, GntR family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Rmet_5213</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="6">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="6">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12072.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519617.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5213"/>
    <dbReference type="EnsemblBacteria" id="ABF12072">
        <property type="protein sequence ID" value="ABF12072"/>
        <property type="gene ID" value="Rmet_5213"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5213"/>
    <dbReference type="eggNOG" id="COG1802">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017584_5_1_4"/>
    <dbReference type="OMA" id="RRMRAHM"/>
    <dbReference type="OrthoDB" id="1911275at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07377">
        <property type="entry name" value="WHTH_GntR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.530">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011711">
        <property type="entry name" value="GntR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008920">
        <property type="entry name" value="TF_FadR/GntR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000524">
        <property type="entry name" value="Tscrpt_reg_HTH_GntR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07729">
        <property type="entry name" value="FCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00392">
        <property type="entry name" value="GntR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00035">
        <property type="entry name" value="HTHGNTR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00895">
        <property type="entry name" value="FCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00345">
        <property type="entry name" value="HTH_GNTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48008">
        <property type="entry name" value="SSF48008"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50949">
        <property type="entry name" value="HTH_GNTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0614">Plasmid</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH gntR-type" evidence="4">
        <location>
            <begin position="2"/>
            <end position="69"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABF12072.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="221" mass="24369" checksum="E45BF3BA85447968" modified="2006-05-30" version="1">MTTIASQIGQQLADEIISGALPPGAKLEEPALAERFQVSRTPVREALRLLDARGLIELVPRRGGVVINTSPEQLADMLEALCELESLCCRIATQRMSAMQRRQLELLHEEAISAAEKHDSDAYLTLNHRFHQLICQGTQNATLVAAVESLRERLAPFRAAQSGVERRFEVSHREHQQIVEAVLGGHPEQAYLAMRSHTTRLTLHVMELIQSKQGGGAKSAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LCS2</accession>
    <name>Q1LCS2_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Outer membrane protein, porin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">Rmet_5195</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="10">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="10">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12054.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5195"/>
    <dbReference type="EnsemblBacteria" id="ABF12054">
        <property type="protein sequence ID" value="ABF12054"/>
        <property type="gene ID" value="Rmet_5195"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5195"/>
    <dbReference type="eggNOG" id="COG3203">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038238_1_1_4"/>
    <dbReference type="OMA" id="IGARWNI"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009279">
        <property type="term" value="C:cell outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046930">
        <property type="term" value="C:pore complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015288">
        <property type="term" value="F:porin activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034220">
        <property type="term" value="P:ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00342">
        <property type="entry name" value="gram_neg_porins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.160.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033900">
        <property type="entry name" value="Gram_neg_porin_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023614">
        <property type="entry name" value="Porin_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001702">
        <property type="entry name" value="Porin_Gram-ve"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002299">
        <property type="entry name" value="Porin_Neis"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13609">
        <property type="entry name" value="Porin_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00182">
        <property type="entry name" value="ECOLNEIPORIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00184">
        <property type="entry name" value="NEISSPPORIN"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0406">Ion transport</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0614">Plasmid</keyword>
    <keyword evidence="5" id="KW-0626">Porin</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1134">Transmembrane beta strand</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="41"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="domain" description="Porin_4" evidence="8">
        <location>
            <begin position="47"/>
            <end position="351"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF13609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ABF12054.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="382" mass="41980" checksum="D05BA00AEDA95883" modified="2006-05-30" version="1">MAGGGACGDDQRPTLAALPCGWTLPATQETKRFGDTMKRKLLVPGAMLFACAGAYAQSAVWLSGYVDLNIEHLIQSGPASVTRMTSGGLNNSRFNLSGVEDLGGGYKAVFTIEPMFSADTGVQSAQFRQSFVGLKSADLGEVTMGRQFTPSYWIAGYADPSWAAAYSMVNNMEFFYASYRVDNAIQYKTPTFYGFTGRVMYAFGLEDTTRAGRFLSIGVDYRNGPLFLGAVSEQQYTRDIHQASQIPSSRDNYFSAVYRIGGFEPTAIFHTYKGYYAYPPYVAFNSSGWDAQIGARWNIDGRSRLYGSVVYRHDDNNKSITSATGFVIGYIYGLSKRTDLYATYAHVHNQNTVPIAYPVTFQSNPSPGQNPSGMQLGIRHAF</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="92" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LCX5</accession>
    <name>Q1LCX5_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Putative acyl-CoA dehydrogenase</fullName>
            <ecNumber evidence="6">1.3.99.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Rmet_5142</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="7">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="7">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the acyl-CoA dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.3.99.-" evidence="6"/>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12001.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5142"/>
    <dbReference type="EnsemblBacteria" id="ABF12001">
        <property type="protein sequence ID" value="ABF12001"/>
        <property type="gene ID" value="Rmet_5142"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5142"/>
    <dbReference type="eggNOG" id="COG1960">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018204_5_2_4"/>
    <dbReference type="OMA" id="GWSRDVW"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016627">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-CH group of donors"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.540.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.110.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046373">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036250">
        <property type="entry name" value="AcylCo_DH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009075">
        <property type="entry name" value="AcylCo_DH/oxidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013786">
        <property type="entry name" value="AcylCoA_DH/ox_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037069">
        <property type="entry name" value="AcylCoA_DH/ox_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009100">
        <property type="entry name" value="AcylCoA_DH/oxidase_NM_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00441">
        <property type="entry name" value="Acyl-CoA_dh_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02771">
        <property type="entry name" value="Acyl-CoA_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47203">
        <property type="entry name" value="SSF47203"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56645">
        <property type="entry name" value="SSF56645"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0274">FAD</keyword>
    <keyword evidence="3" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-0614">Plasmid</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Acyl-CoA_dh_N" evidence="5">
        <location>
            <begin position="24"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA_dh_1" evidence="4">
        <location>
            <begin position="252"/>
            <end position="370"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF02771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABF12001.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="392" mass="42048" checksum="A26ADE99377C5CEC" modified="2006-05-30" version="1">MAGAEQNDCVSSPPRSQHVDFRFSEEQAMLRDTLARYLSDHYTFDARQAAVHSAAGWRPDCWRAFARDLGLLGIGIPERFGGLGGGIIDHIVVMEQFGRHLVLEPYLGTAILAVDALQYANETLAAEALPAIAAGELVVGWAHAEPESRYGMMVRTRADAAAGGYRLHGRKALVTGAPWATHFIVSANTEAGPTLFWVERTAPGLTLIEAPTFDGGRTADLILTDVPVSAAQVLGMPGGALPLLEQLGDTAAVALCAEATGATARMLENTIEYARQRQQFGQAIGSFQALQHRMADMYMLHEQAVALTQVAAMRLAGEPHERALGASTAKAHVDEAGRRIGQGAVQIHGGMGVTEETAVGHYFKRVTAIGLQFGNVAYHLRRCVDLRYPANA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="100" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LCY8</accession>
    <name>Q1LCY8_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Histidine kinase</fullName>
            <ecNumber evidence="2">2.7.13.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Rmet_5129</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="6">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="6">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.</text>
            <dbReference type="EC" id="2.7.13.3"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.7.13.3" evidence="2"/>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11988.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519535.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5129"/>
    <dbReference type="EnsemblBacteria" id="ABF11988">
        <property type="protein sequence ID" value="ABF11988"/>
        <property type="gene ID" value="Rmet_5129"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5129"/>
    <dbReference type="eggNOG" id="COG0642">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_42_3_4"/>
    <dbReference type="OMA" id="NACKWAD"/>
    <dbReference type="OrthoDB" id="692375at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036097">
        <property type="entry name" value="HisK_dim/P_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004358">
        <property type="entry name" value="Sig_transdc_His_kin-like_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00344">
        <property type="entry name" value="BCTRLSENSOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47384">
        <property type="entry name" value="SSF47384"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="SSF55874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0614">Plasmid</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="6"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="164"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="4">
        <location>
            <begin position="244"/>
            <end position="442"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABF11988.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="448" mass="48694" checksum="6F6EB0C499A26D99" modified="2006-05-30" version="1">MKRPTIAGRLLLIATLWVVGTLCATGWVLDTLFGRHVDKTYVTELDRDLTSLAAALEWNGHGQLELTRQPADPRFERPYSGAYWQAASGTQSLRSRSLWDIDIPTTIERKIPAGDADIRNGPEGQSLLAKSRRLQLTGDDAYVQITVALDRTELRDAKRSFRHLLWLSLGILGVGILGAVWAQIRFGLAPLSRLRAAVAKLHGGGDPTLQGVWPAEVKPLVDEINVLLKRNHDAVERSRRQAADLAHAVKTPLAILANSATALPGPLSQSVLQQVDAMRQQVDRHLSRARAVGAAGAGGRSVAIRPAVEELARALVRLHAGKPIEVSVEGNADFFGDRQDLVEMLGNLLDNAWKWASTRIRVTLVSQESEIEICVDDDGPGMPASSMHQAMQPFSRLDESVEGSGLGLSIVRDICAMYDGAFELKRSSLGGLAARMRFPAAGATQSQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="99" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LDE1</accession>
    <name>Q1LDE1_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">NTP-binding protein TniB (Tn6048)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">tniB</name>
        <name evidence="1" type="ordered locus">Rmet_3009</name>
        <name evidence="2" type="ordered locus">Rmet_4044</name>
        <name evidence="3" type="ordered locus">Rmet_4973</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid</name>
    </geneLocation>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="submission" date="2006-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of the chromosome of Ralstonia metallidurans CH34.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Copeland A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Barry K."/>
                <person name="Detter J.C."/>
                <person name="Glavina del Rio T."/>
                <person name="Hammon N."/>
                <person name="Israni S."/>
                <person name="Dalin E."/>
                <person name="Tice H."/>
                <person name="Martinez M."/>
                <person name="Goltsman E."/>
                <person name="Pitluck S."/>
                <person name="Schmutz J."/>
                <person name="Larimer F."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Kim E."/>
                <person name="Mergeay M."/>
                <person name="Benotmane M.A."/>
                <person name="Vallaeys T."/>
                <person name="Michaux A."/>
                <person name="Monchy S."/>
                <person name="Dunn J."/>
                <person name="McCorkle S."/>
                <person name="Taghavi S."/>
                <person name="van der Lelie D."/>
                <person name="Richardson P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">CH34</strain>
            <plasmid evidence="3">megaplasmid</plasmid>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2010-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Benotmane R."/>
                <person name="Lapidus A."/>
                <person name="McCorkle S."/>
                <person name="Monchy S."/>
                <person name="Taghavi S."/>
                <person name="van der Lelie N."/>
                <person name="Dunn J."/>
                <person name="Leys N."/>
                <person name="Mergeay M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">CH34</strain>
            <plasmid evidence="3">megaplasmid</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09882.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10912.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11835.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_009687862.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3009"/>
    <dbReference type="EnsemblBacteria" id="ABF09882">
        <property type="protein sequence ID" value="ABF09882"/>
        <property type="gene ID" value="Rmet_3009"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF10912">
        <property type="protein sequence ID" value="ABF10912"/>
        <property type="gene ID" value="Rmet_4044"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF11835">
        <property type="protein sequence ID" value="ABF11835"/>
        <property type="gene ID" value="Rmet_4973"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3009"/>
    <dbReference type="KEGG" id="rme:Rmet_4044"/>
    <dbReference type="KEGG" id="rme:Rmet_4973"/>
    <dbReference type="eggNOG" id="COG2842">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_067529_2_0_4"/>
    <dbReference type="OMA" id="PQHASRF"/>
    <dbReference type="OrthoDB" id="471286at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008868">
        <property type="entry name" value="TniB"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05621">
        <property type="entry name" value="TniB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF09882.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF10912.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF11835.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="302" mass="34188" checksum="C9A681E0379754B0" modified="2006-05-30" version="1">MEEYPIIDLSHLLPAAQGLARLPADERIHRLRADRWIGYPRAVEALNRLEILYTWPNKQRMPNLLLVGPTNNGKSMIVEKFRRTHPARADADQEHIPVLVVQMPSEPSVIRFYVALLAAMGAPLRPRPRLPEMEQLALALLRKVGVRMLVIDELHNVLAGNSVNRREFLNLLRFLGNELRIPLVGVGTRDAYLAIRSDDQLENRFEPMMLPVWEANHDCCSLLASFAASLPLRRPSSIATLDMARYLLTRSEGTIGELAHLLMAAAIVAVESGEEAINHRTLSMADYTGPSERRRQFERELM</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-10-07" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LDE5</accession>
    <name>Q1LDE5_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Extra-cytoplasmic solute receptor UPF0065</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">bug</name>
        <name evidence="5" type="ordered locus">Rmet_4969</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="6">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="6">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Periplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the UPF0065 (bug) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11831.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4969"/>
    <dbReference type="EnsemblBacteria" id="ABF11831">
        <property type="protein sequence ID" value="ABF11831"/>
        <property type="gene ID" value="Rmet_4969"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4969"/>
    <dbReference type="eggNOG" id="COG3181">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045683_0_0_4"/>
    <dbReference type="OMA" id="GPGNIAH"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042597">
        <property type="term" value="C:periplasmic space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005064">
        <property type="entry name" value="BUG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042100">
        <property type="entry name" value="Bug_dom1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42928">
        <property type="entry name" value="PTHR42928"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03401">
        <property type="entry name" value="TctC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017082">
        <property type="entry name" value="YflP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0614">Plasmid</keyword>
    <keyword evidence="5" id="KW-0675">Receptor</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="39"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABF11831.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="364" mass="39013" checksum="8E8B1A3DE2EDA1A8" modified="2006-05-30" version="1">MTQELSRTTENDIENDKETHMKEREITARRRAIKGGMKLVAAALAVMAGTMAAPAGAQTATQTANWPNKPIRIVVPFPAGSTTDTVARVLSEQLSKSLGQPVIVENKPGANGLIGVAEVAKSPADGYTLLVTNSSSITINPQVYKRSNYKPKDLAPVALVVEAPFVLVANPEWAKKNSIGSVGDLVRYATRNPGKLSYGSAGPGNIAHLSFAMLNNREKIQTTHVPYKSASQAEMAVISGELETAFDAWNGLPQIKAGKLKPLAVTSPKRMPQLPDVPSLEEAGVKPFDVSFWLGMLAPAGTPPQIVQKLYEITKGVANDARAKSVLSQQGEVVMIDPANFAKRVEKEEANWAVVIQREGITLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LDK5</accession>
    <name>Q1LDK5_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">TetR/AcrR family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_4909</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11771.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519330.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4909"/>
    <dbReference type="EnsemblBacteria" id="ABF11771">
        <property type="protein sequence ID" value="ABF11771"/>
        <property type="gene ID" value="Rmet_4909"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4909"/>
    <dbReference type="eggNOG" id="COG1309">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069356_28_4_4"/>
    <dbReference type="OMA" id="RRYWEDT"/>
    <dbReference type="OrthoDB" id="2047542at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001647">
        <property type="entry name" value="HTH_TetR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036271">
        <property type="entry name" value="Tet_transcr_reg_TetR-rel_C_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00440">
        <property type="entry name" value="TetR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00455">
        <property type="entry name" value="HTHTETR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48498">
        <property type="entry name" value="SSF48498"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50977">
        <property type="entry name" value="HTH_TETR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH tetR-type" evidence="2">
        <location>
            <begin position="4"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="H-T-H motif" evidence="1">
        <location>
            <begin position="27"/>
            <end position="46"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF11771.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="201" mass="22132" checksum="2AD41316D443F823" modified="2006-05-30" version="1">MKTSSVREQLLEHASVLMRRRGYNGFSYRDLAELVGVKTSSIHYYFPSKDDLVLEVVRQYRQTSVARLAAIDTSLPLIEQARKYVLPLSNGIDLGQICLIGMLAAEVMVLPQAVRALLQEHFQANENFIADMLRRAEAERGQQYPVPPQLLAKVLYGAIQNGLITARMSGSNDRIDAAANMLIGAVGGSMCAIEEHEAVAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="92" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LDL2</accession>
    <name>Q1LDL2_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Short-chain dehydrogenase/reductase SDR</fullName>
            <ecNumber evidence="1">1.1.1.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_4902</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EC" id="1.1.1.-" evidence="1"/>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11764.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519324.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4902"/>
    <dbReference type="EnsemblBacteria" id="ABF11764">
        <property type="protein sequence ID" value="ABF11764"/>
        <property type="gene ID" value="Rmet_4902"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4902"/>
    <dbReference type="eggNOG" id="COG1028">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010194_1_1_4"/>
    <dbReference type="OMA" id="GICEFKE"/>
    <dbReference type="OrthoDB" id="1294334at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF11764.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="254" mass="26064" checksum="152CA391B9F70348" modified="2006-05-30" version="1">MYKLFDMSGRVAVVTGSTKGMGLEMARALGASGARVVVSGRNAAISEKVAAALKSEGIDATGLACDIADTASVRAFADRVLETFGRVDALVLNAAGSGVAGSILEQGPEDFDAVMAGNVRGNLVLVNALAPQMISRNDGSIIFMSSIAARRGSALLPLYSISKGAVDLAMRSLAHTLGPHNINVNSINPGPVRTDFSRDALWGNPDREKALSASIPMRRIAEARDVAGLAVLLASPAGRYISGQTIGLDGGATA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="98" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LDN4</accession>
    <name>Q1LDN4_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Transcriptional regulator, LysR family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">catM</name>
        <name evidence="3" type="ordered locus">Rmet_4880</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the LysR transcriptional regulatory family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11742.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519301.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4880"/>
    <dbReference type="EnsemblBacteria" id="ABF11742">
        <property type="protein sequence ID" value="ABF11742"/>
        <property type="gene ID" value="Rmet_4880"/>
    </dbReference>
    <dbReference type="GeneID" id="60823866"/>
    <dbReference type="KEGG" id="rme:Rmet_4880"/>
    <dbReference type="eggNOG" id="COG0583">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039613_6_4_4"/>
    <dbReference type="OMA" id="YKYALQM"/>
    <dbReference type="OrthoDB" id="1439189at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005119">
        <property type="entry name" value="LysR_subst-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000847">
        <property type="entry name" value="Tscrpt_reg_HTH_LysR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00126">
        <property type="entry name" value="HTH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03466">
        <property type="entry name" value="LysR_substrate"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00039">
        <property type="entry name" value="HTHLYSR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50931">
        <property type="entry name" value="HTH_LYSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH lysR-type" evidence="2">
        <location>
            <begin position="1"/>
            <end position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF11742.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="305" mass="34953" checksum="5B093E145C07F8E1" modified="2006-05-30" version="1">MELRHLRYFVAVAEERNFTRAAERLHIAQPPLSRQIQQLEESLGVLLFERNSRPLKLTDTGRFFYTHALQLLAQTTELEAMMRRVGKIERAMSIGFVGSTLYGMLPRIIRRFRTEHAEIELTLHEMSTMDQIKALKEGRIDVGFGRIRHEDPAVRRVVLREERMIVALPVGHPLSIGRKMLALPDLLEDTLIIFPKAPRPSFADQVLAAFHDRALRPGRIHEVRELQIALGLVAMGEGISVVPSSVYGLKRDDVTYVELDDAKLVSPIIMSMRTLDESEDLRAMLDMIYRLYEEIGMEYIPPAQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="99" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LDP3</accession>
    <name>Q1LDP3_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transcriptional regulator, AraC family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_4871</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11733.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519292.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4871"/>
    <dbReference type="EnsemblBacteria" id="ABF11733">
        <property type="protein sequence ID" value="ABF11733"/>
        <property type="gene ID" value="Rmet_4871"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4871"/>
    <dbReference type="eggNOG" id="COG4977">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_59_0_4"/>
    <dbReference type="OMA" id="CGYSDAA"/>
    <dbReference type="OrthoDB" id="1459004at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018060">
        <property type="entry name" value="HTH_AraC"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12833">
        <property type="entry name" value="HTH_18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00342">
        <property type="entry name" value="HTH_ARAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="SSF52317"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01124">
        <property type="entry name" value="HTH_ARAC_FAMILY_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH araC/xylS-type" evidence="1">
        <location>
            <begin position="237"/>
            <end position="335"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS01124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF11733.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="361" mass="40202" checksum="4A5DAF0087529395" modified="2006-05-30" version="1">MADPATTSNHPLHVKLLWLPDAMPGTLFGALDVLRTAAGVAQLQRPGESNLLTWEIIGGNGRALPAPLPGLQPVPARRGQRHGRTLMIVPGLMAHSAPHLSEMVTNDTVTQRTIARHVDAGGWLAAIYSGMVYPLALGLLDGARCGAPWMFQSSLAQRFPGCDLSSDEPIGLHERVFHCVAPALLTEFMLRVLGHLHDPDLAQTVSRLLLYQQERQQWVPELVSQRWLGKTTDSPVYRAIGWLQAHVDEPYRLGAVAEAAAVSERTLLRHFRQVTGQTPLDYLHRLRVERARMLLEVTLHGTQAIAEACGYSDAAAFRRLFQRAMQMSMSEYRTRFALRTRRRYWRVEGQAARRGRAAPQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LE88</accession>
    <name>Q1LE88_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">3-oxoadipate enol-lactonase</fullName>
            <ecNumber evidence="2">3.1.1.24</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">catD2</name>
        <name evidence="2" type="ordered locus">Rmet_4676</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EC" id="3.1.1.24" evidence="2"/>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11538.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519111.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4676"/>
    <dbReference type="ESTHER" id="ralme-q1le88">
        <property type="family name" value="Carboxymethylbutenolide_lactonase"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF11538">
        <property type="protein sequence ID" value="ABF11538"/>
        <property type="gene ID" value="Rmet_4676"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4676"/>
    <dbReference type="eggNOG" id="COG2267">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020336_50_3_4"/>
    <dbReference type="OMA" id="MWRWQRE"/>
    <dbReference type="OrthoDB" id="1196738at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047570">
        <property type="term" value="F:3-oxoadipate enol-lactonase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042952">
        <property type="term" value="P:beta-ketoadipate pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026968">
        <property type="entry name" value="PcaD/CatD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00561">
        <property type="entry name" value="Abhydrolase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00111">
        <property type="entry name" value="ABHYDROLASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="SSF53474"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02427">
        <property type="entry name" value="protocat_pcaD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AB hydrolase-1" evidence="1">
        <location>
            <begin position="22"/>
            <end position="244"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF11538.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="259" mass="28037" checksum="00CEC45D45AD0F7C" modified="2006-05-30" version="1">MPFADLSDVRIHYRLDGAEHLPVLVLSNSLGTTLDMWQPQIAALSQHFRVLRYDTRGHGESGVTPGPYSIAQLGGDVIGLLDHLGLQRVHFCGLSMGGITGMWLALHHADRLNRLVLCNTAAYIGPPENWTNRAALVEREGMAAITAAVVDKWLTPDFSAQQPELVQRLQTMLGATSPSGYAANCLAVRDSDLRAEIQRIQTPTLVIAGSGDIPTPPADGRYLADTIPGARYTELYAAHLSNLQQVEGFTQAVLTFLGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="62" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LED3</accession>
    <name>Q1LED3_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_4631</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11493.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008646114.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4631"/>
    <dbReference type="EnsemblBacteria" id="ABF11493">
        <property type="protein sequence ID" value="ABF11493"/>
        <property type="gene ID" value="Rmet_4631"/>
    </dbReference>
    <dbReference type="GeneID" id="60821801"/>
    <dbReference type="KEGG" id="rme:Rmet_4631"/>
    <dbReference type="eggNOG" id="COG3246">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_065536_2_0_4"/>
    <dbReference type="OMA" id="KMSTVKQ"/>
    <dbReference type="OrthoDB" id="1892901at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019475">
        <property type="term" value="P:L-lysine catabolic process to acetate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008567">
        <property type="entry name" value="KCE_family"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37418">
        <property type="entry name" value="PTHR37418"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05853">
        <property type="entry name" value="BKACE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF11493.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="352" mass="39052" checksum="C4E205ECFAAEC15C" modified="2006-05-30" version="1">MNFLDGHLFPENQPPLIITAAPYAPGWLPEDFPEEIPVSMEAQIQKAVDCYNAGATVLHLHVRELDGKGSKRLSKFNELIAGVRKAVPEMIIQVGGSISFAPEDEGQAAKWLSDDTRHMLADLEPTPDQVTVTVNTSQMNVTDQAEDADFKGTSRERAAIFNAYKEMTVPAQPGWVEEHVRRLTAKGIQSAFQCYNLNSFESVERLMRRGFYKGPLVMNWVAIAGGMDTPSVYSLANFIRAVPDGAVVTVESNVRNVLPVNMMGISMGLHVRCGTEDCLWNQSRTEKMSTVKQIEQLVRISREFGREIATAKQARQISRIGEFYDTVEESLQANGFAPNRNGGNQGFLRKTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="113" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LEK0</accession>
    <name>Q1LEK0_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase</fullName>
            <ecNumber evidence="10">2.1.1.14</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Cobalamin-independent methionine synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">Methionine synthase, vitamin-B12 independent isozyme</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10 13" type="primary">metE</name>
        <name evidence="13" type="ordered locus">Rmet_4564</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="14">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="14">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 10">Catalyzes the transfer of a methyl group from 5-methyltetrahydrofolate to homocysteine resulting in methionine formation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>5-methyltetrahydropteroyltri-L-glutamate + L-homocysteine = L-methionine + tetrahydropteroyltri-L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:21196"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:58140"/>
            <dbReference type="ChEBI" id="CHEBI:58199"/>
            <dbReference type="ChEBI" id="CHEBI:58207"/>
            <dbReference type="EC" id="2.1.1.14"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="10">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 10">Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-methionine from L-homocysteine (MetE route): step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the vitamin-B12 independent methionine synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.14" evidence="10"/>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11426.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519014.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4564"/>
    <dbReference type="EnsemblBacteria" id="ABF11426">
        <property type="protein sequence ID" value="ABF11426"/>
        <property type="gene ID" value="Rmet_4564"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4564"/>
    <dbReference type="eggNOG" id="COG0620">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013175_0_0_4"/>
    <dbReference type="OMA" id="NDILPWI"/>
    <dbReference type="OrthoDB" id="577156at2"/>
    <dbReference type="UniPathway" id="UPA00051">
        <property type="reaction ID" value="UER00082"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003871">
        <property type="term" value="F:5-methyltetrahydropteroyltriglutamate-homocysteine S-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009086">
        <property type="term" value="P:methionine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03311">
        <property type="entry name" value="CIMS_C_terminal_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.210">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00172">
        <property type="entry name" value="Meth_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013215">
        <property type="entry name" value="Cbl-indep_Met_Synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006276">
        <property type="entry name" value="Cobalamin-indep_Met_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002629">
        <property type="entry name" value="Met_Synth_C/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038071">
        <property type="entry name" value="UROD/MetE-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08267">
        <property type="entry name" value="Meth_synt_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01717">
        <property type="entry name" value="Meth_synt_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000382">
        <property type="entry name" value="MeTrfase_B12_ind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51726">
        <property type="entry name" value="SSF51726"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01371">
        <property type="entry name" value="met_syn_B12ind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 10" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="7 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9 10" id="KW-0486">Methionine biosynthesis</keyword>
    <keyword evidence="4 10 13" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="13" id="KW-0614">Plasmid</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 10 13" id="KW-0808">Transferase</keyword>
    <keyword evidence="8 10" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Meth_synt_1" evidence="12">
        <location>
            <begin position="4"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="domain" description="Meth_synt_2" evidence="11">
        <location>
            <begin position="433"/>
            <end position="755"/>
        </location>
    </feature>
    <feature type="region of interest" description="5-methyltetrahydropteroyltri-L-glutamate binding" evidence="10">
        <location>
            <begin position="16"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="region of interest" description="L-homocysteine binding" evidence="10">
        <location>
            <begin position="438"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="region of interest" description="L-methionine binding" evidence="10">
        <location>
            <begin position="438"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="region of interest" description="5-methyltetrahydropteroyltri-L-glutamate binding" evidence="10">
        <location>
            <begin position="522"/>
            <end position="523"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="10">
        <location>
            <position position="701"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="112"/>
        </location>
        <ligand>
            <name>5-methyltetrahydropteroyltri-L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:58207"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="491"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="568"/>
        </location>
        <ligand>
            <name>5-methyltetrahydropteroyltri-L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:58207"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="606"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="612"/>
        </location>
        <ligand>
            <name>5-methyltetrahydropteroyltri-L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:58207"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="648"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="650"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="672"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="733"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004681"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF01717"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF08267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ABF11426.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="803" mass="88816" checksum="591D53ED90AFCB80" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LEQ9</accession>
    <name>Q1LEQ9_CUPMC</name>
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        <submittedName>
            <fullName evidence="3">Long-chain-fatty-acid-CoA ligase (Acyl-CoA synthetase (AMP-forming)/AMP-acid ligase II)</fullName>
            <ecNumber evidence="3">2.3.1.86</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">fabD</name>
        <name evidence="3" type="ordered locus">Rmet_4502</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="STRING" id="266264.Rmet_4502"/>
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        <property type="gene ID" value="Rmet_4502"/>
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    <dbReference type="HOGENOM" id="CLU_000022_59_9_4"/>
    <dbReference type="OMA" id="ERIMAWC"/>
    <dbReference type="OrthoDB" id="961884at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
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        <property type="term" value="F:fatty-acyl-CoA synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
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        <property type="term" value="F:ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="AMP_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="3" id="KW-0436">Ligase</keyword>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
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        <location>
            <begin position="35"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="domain" description="AMP-binding_C" evidence="2">
        <location>
            <begin position="472"/>
            <end position="551"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF11367.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="564" mass="61696" checksum="CCD8EEDC0CFF3B90" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="60" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LER9</accession>
    <name>Q1LER9_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_4492</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11357.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="EnsemblBacteria" id="ABF11357">
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        <property type="gene ID" value="Rmet_4492"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4492"/>
    <dbReference type="HOGENOM" id="CLU_533025_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="352"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="352"/>
            <end position="380"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF11357.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="511" mass="53691" checksum="B64CC5B1A0A0D7FA" modified="2006-05-30" version="1">MRPLPSSLLLAILRRIPAPSGLLPRILSLPRIVFDLLLAPVLMTVSVLASLLLPIQAHAQAAGGAVVDYAYQRTMSRIVAGSAAGLQMSSPTTMNIINAGKVVGSFAVTEKRLIDLATLAKYARASTVPGIIALAGTELLAYGLEKCADGTWCTREANKPNGSNVDWGSTGWMTAGGASCTSYSCSAEAACRAAPNIANGKYPLLKYIVLNGGTQAYCYSRNAADNPLSEGGWGLIQRQSSYVDPPGSLVPASDANIETAWKGAFQAKPFLQEKVWGFEDQSTQNQQWADALAQLAAVAAPSTVTVADPTTSTTTPTGTTKVDRSCTYAASANADAGSQKDKPASVGVTCTTTTTAPDGTVTQQTTKTDASPQGGQTVPQKVDIDTCGLPGKPACKIDETGTKAQSDVDTALQQRSSDMDSRLSITQQAANDSLSEVERKHDSVHIFTLFNVFPTFASEQCENPTVPGVVGGSLPVDICTYYYMLKDVMGFGVALFVLIDSVQVVREAVKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-10-07" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LF17</accession>
    <name>Q1LF17_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Transcriptional regulator, IclR family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Rmet_4394</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="7">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="7">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11259.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4394"/>
    <dbReference type="EnsemblBacteria" id="ABF11259">
        <property type="protein sequence ID" value="ABF11259"/>
        <property type="gene ID" value="Rmet_4394"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4394"/>
    <dbReference type="eggNOG" id="COG1414">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_062618_3_1_4"/>
    <dbReference type="OMA" id="GRRQGYY"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029016">
        <property type="entry name" value="GAF-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014757">
        <property type="entry name" value="Tscrpt_reg_IclR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005471">
        <property type="entry name" value="Tscrpt_reg_IclR_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09339">
        <property type="entry name" value="HTH_IclR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01614">
        <property type="entry name" value="IclR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00346">
        <property type="entry name" value="HTH_ICLR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51077">
        <property type="entry name" value="HTH_ICLR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51078">
        <property type="entry name" value="ICLR_ED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0614">Plasmid</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH iclR-type" evidence="4">
        <location>
            <begin position="95"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="domain" description="IclR-ED" evidence="5">
        <location>
            <begin position="158"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="64"/>
            <end position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABF11259.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="336" mass="36823" checksum="D7193B4527AEC964" modified="2006-05-30" version="1">MAVPARMAEILTRHSKRGGCCCFDVAQTRSSLKSSRLREPALAALQPPSQPHRPDIRARATVRRVSDATFRRPKTPPAMPERPAPSAAKPSSMNVKTALRVIEIIETFARERRALSLSELARLLAVPASSCLALIRTLTSLGYLYETARRQGYYPTGRLLAMAQQIARHDPVLDRVHGTMSELRDATGETIVIGKLHEGQAVVYLDVLESPHAIRYIATAGERRELHANSIGKALLAAMDEAERATLLAQLPFQAFTSRTLTTPAALEADLQQARERGAYVNLSESMPDVGALAWPVRLSGECYAISIAGPVYRIEPHLDRYAPMLRAACAALEKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LFR4</accession>
    <name>Q1LFR4_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Acyl-CoA transferase/L-carnitine dehydratase, CaiB</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">caiB1</name>
        <name evidence="1" type="ordered locus">Rmet_4145</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11012.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4145"/>
    <dbReference type="EnsemblBacteria" id="ABF11012">
        <property type="protein sequence ID" value="ABF11012"/>
        <property type="gene ID" value="Rmet_4145"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4145"/>
    <dbReference type="eggNOG" id="COG1804">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033975_0_0_4"/>
    <dbReference type="OMA" id="MGNENFT"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1540.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10540">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003673">
        <property type="entry name" value="CoA-Trfase_fam_III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044855">
        <property type="entry name" value="CoA-Trfase_III_dom3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023606">
        <property type="entry name" value="CoA-Trfase_III_dom_1_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02515">
        <property type="entry name" value="CoA_transf_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89796">
        <property type="entry name" value="SSF89796"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF11012.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="414" mass="44560" checksum="F5120E4A410A02A8" modified="2006-05-30" version="1">MNKTNNMPGRTHIPLMQKPLRNIRVLDLTNVLAGPFCCHQLAHMGAEVIKVETPGTGDLARQLGADAELNRKLMGVSFLAQNPGKRSITINLKHARGKEVFRKLVQSADVVVENFRPGVMTRLGLGYDELKLVNPRLIYCAISGFGQDGPLSDLPAYDQIIQGMAGVMSITGDPQTAPYRVGYPVSDTIGGMTAAFSIAASLADTERTEGYFLDVSMLEATLVTMGWVVSNHLVAGKDPAPMGNENMTASPSGTFRTGDGPLNIAANKQEQFEAVCRVVGRPELATDPRFAERQSRLANRAALTAELEAELAKKSATDWWQLLNEAGVPAGPVLKVPDTLALPQVRDRGMIGAFENVPGVGRDIRLLRTGFKVNGQAPSVDTPPPQLGEHTDDILGELGYDAAAIDTLKQERAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LFR9</accession>
    <name>Q1LFR9_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative methyltransferase</fullName>
            <ecNumber evidence="2">2.1.1.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_4140</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EC" id="2.1.1.-" evidence="2"/>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11007.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518632.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4140"/>
    <dbReference type="EnsemblBacteria" id="ABF11007">
        <property type="protein sequence ID" value="ABF11007"/>
        <property type="gene ID" value="Rmet_4140"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4140"/>
    <dbReference type="eggNOG" id="COG2226">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_049344_3_0_4"/>
    <dbReference type="OMA" id="GLWRATF"/>
    <dbReference type="OrthoDB" id="1687413at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013216">
        <property type="entry name" value="Methyltransf_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08241">
        <property type="entry name" value="Methyltransf_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Methyltransf_11" evidence="1">
        <location>
            <begin position="47"/>
            <end position="135"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF08241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF11007.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="255" mass="27397" checksum="E43F961C8D28053E" modified="2006-05-30" version="1">MTAIHHAAAQGFSAQADTYARGRPEYPAELSGWLRDTLGVAPGKSVVDLGAGTGKFTRLLAQTGATVIAVEPVDAMRAQLSSKLPDVQALAGSAESIPLPDGSVDAVVCAQAFHWFANTAAVQEIRRVLKPGGKLGLVWNVRDESVGWVARLTEIMTPFEGDAPRFYKGDWKKVFPADGMGPLGLTRFAYTHSGAPEQVIVDRVMSVSFIASLPQDQQDAVRARLREVIATDPALQGHDVVSFPYSTEAYCCERL</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LFU8</accession>
    <name>Q1LFU8_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative sulferase (MOSC domain)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_4111</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10978.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518606.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="SMR" id="Q1LFU8"/>
    <dbReference type="STRING" id="266264.Rmet_4111"/>
    <dbReference type="EnsemblBacteria" id="ABF10978">
        <property type="protein sequence ID" value="ABF10978"/>
        <property type="gene ID" value="Rmet_4111"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4111"/>
    <dbReference type="eggNOG" id="COG2258">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_104911_0_0_4"/>
    <dbReference type="OMA" id="PPCAHVE"/>
    <dbReference type="OrthoDB" id="1877633at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030151">
        <property type="term" value="F:molybdenum ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005302">
        <property type="entry name" value="MoCF_Sase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011037">
        <property type="entry name" value="Pyrv_Knase-like_insert_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03473">
        <property type="entry name" value="MOSC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50800">
        <property type="entry name" value="SSF50800"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51340">
        <property type="entry name" value="MOSC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MOSC" evidence="1">
        <location>
            <begin position="38"/>
            <end position="174"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51340"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF10978.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="174" mass="19356" checksum="86537B4AE68C2419" modified="2006-05-30" version="1">MEETVSQTSDRSVGATAPSWRGVVRFLHKTPRAFLPMLPFPEITLVAGKGIEGDRYMIGNEAGFYSHKPEEGRQVTLFELETLEALARDAKVVLHPEEHRRNVTVEGVPLNHLVGRRFWLGETLLEATRLSTPCRHLEEILGKAVFDPLVHRSGLNCKILSGGVVRIGDVVRPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LFY7</accession>
    <name>Q1LFY7_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Putative formyl-CoA transferases/L-carnitine dehydratase</fullName>
            <ecNumber evidence="1">2.8.3.16</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_4071</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EC" id="2.8.3.16" evidence="1"/>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10939.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518565.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4071"/>
    <dbReference type="EnsemblBacteria" id="ABF10939">
        <property type="protein sequence ID" value="ABF10939"/>
        <property type="gene ID" value="Rmet_4071"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4071"/>
    <dbReference type="eggNOG" id="COG1804">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033975_2_1_4"/>
    <dbReference type="OMA" id="PYTDAHW"/>
    <dbReference type="OrthoDB" id="969868at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033608">
        <property type="term" value="F:formyl-CoA transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1540.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10540">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003673">
        <property type="entry name" value="CoA-Trfase_fam_III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044855">
        <property type="entry name" value="CoA-Trfase_III_dom3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023606">
        <property type="entry name" value="CoA-Trfase_III_dom_1_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02515">
        <property type="entry name" value="CoA_transf_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89796">
        <property type="entry name" value="SSF89796"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF10939.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="391" mass="42752" checksum="4D96AE5718260118" modified="2006-05-30" version="1">MLPLSGIRVVDLSTVVMGPYASQWLADLGAEVIKVETPEGDSTRRTGPATEDGMSAIFLGVNRSKKSVVLNLKRPEAQAALHRLLASADVFVHSMRPQKLAKLDLDPASVMARHPQLIFVSLLGFAEDGPYGGRPAYDDIIQGLCGNAALMAAQTGTVRYFPTISADKTSGLVAALSISAAIAGRERQGGKGSMVEVPMFESMVAFNMVEHLYGQHFEPPRGPSGYPRVLAPLRRPYQTADGYVCMMPYTDAHWRDFFQASGREDLVADARFTDIAARTRHIETLYEITGELVRARSTADWLAICERLQIPVARVNDLHELVDDPHLRATGFFDQIEDPSMGTLRFPGAPVRFDGERARAAVPPRLGEHTREILAAAGLGDDEIDRLQSNQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LG04</accession>
    <name>Q1LG04_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Cytochrome c-type biogenesis protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">cycL</name>
        <name evidence="7" type="ordered locus">Rmet_4054</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="8">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="8">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Possible subunit of a heme lyase.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the CcmH/CycL/Ccl2/NrfF family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10922.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518549.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4054"/>
    <dbReference type="EnsemblBacteria" id="ABF10922">
        <property type="protein sequence ID" value="ABF10922"/>
        <property type="gene ID" value="Rmet_4054"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4054"/>
    <dbReference type="eggNOG" id="COG3088">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG4235">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_823071_0_0_4"/>
    <dbReference type="OMA" id="QNNEFDS"/>
    <dbReference type="OrthoDB" id="1539126at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.640">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005616">
        <property type="entry name" value="CcmH/CycL/Ccl2/NrfF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038297">
        <property type="entry name" value="CcmH/CycL/NrfF/Ccl2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_repeat"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03918">
        <property type="entry name" value="CcmH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="SSF48452"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 6" id="KW-0349">Heme</keyword>
    <keyword evidence="4 6" id="KW-0408">Iron</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0614">Plasmid</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0802">TPR repeat</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5011020595" description="Cytochrome c-type biogenesis protein" evidence="6">
        <location>
            <begin position="23"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="100"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="147"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="5">
        <location>
            <begin position="216"/>
            <end position="249"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU364112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABF10922.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="344" mass="37139" checksum="6313E0DAC860BA8A" modified="2006-05-30" version="1">MPRTWLSTLTLLIALHAGAAFALTDAELDARVLALSSQLRCLVCQNQTLADSNADLAVDLRRQIRAQMAQGASDDAIKTYLVQRYGDFVLYKPPFKPQTWLLWLGPFLLLMAVLAWLWRGRRRQMAAGNAVEETERDMQSPARRQSLLTNAMLFTVLPPVAVALYLHLGSPVSLLGSAVAHDGPHGPDGEVTLTAARVEDMVDQLAARLRKNPNDAEGWVMLGRSYTVLERNDDAAEAFARAVALEPKVASLRSDYADVLASVNGGSLEGPAMAQIDAALRLDPDDPKGLALAASAAAERGDKAGAIAYWEHLYRLLPPDSQTAGRVAANLAAARSVAEPGQRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="67" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LG05</accession>
    <name>Q1LG05_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Rmet_4053</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="8">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="8">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the autoinducer-2 exporter (AI-2E) (TC 2.A.86) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10921.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518548.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4053"/>
    <dbReference type="DNASU" id="4040911"/>
    <dbReference type="EnsemblBacteria" id="ABF10921">
        <property type="protein sequence ID" value="ABF10921"/>
        <property type="gene ID" value="Rmet_4053"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4053"/>
    <dbReference type="eggNOG" id="COG0628">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041771_2_2_4"/>
    <dbReference type="OMA" id="EIAYVYQ"/>
    <dbReference type="OrthoDB" id="944044at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002549">
        <property type="entry name" value="AI-2E-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21716">
        <property type="entry name" value="PTHR21716"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01594">
        <property type="entry name" value="AI-2E_transport"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0614">Plasmid</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="33"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="61"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="159"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="239"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="267"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="309"/>
            <end position="339"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009773"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABF10921.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="361" mass="38451" checksum="3031F12E656FA549" modified="2006-05-30" version="1">MAESPGLYHKTFLLLLAAVSIAFFWILLPFYGAVFWGAVLAIIFAPLQRRLTAGLRGRNNVAALLTLLLVLVLVILPLTIITASLINQGVSLYDSIRSGQLNFGVYFQRAVDALPPYVRTLLAKVDLTSIADLQEKLSASALQASQFVATRALSIGQNTAQFVISFGIMLYLLFFLLRDGPRLSRKIRTAVPLSDLYKQHLIQKFTTVVRATVKGNVAVALVQGTLGGGIFAVLGIQGALLWGVIMAFLSLLPAVGAGLIWAPVAVYFLLTGSTVTGLVLIGFGVLVIGMVDNVLRPILVGKDTQMPDYVVLISTLGGMALFGLNGFVIGPLIAALFIACWDLNAHGIDENGDIGSNGPAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LG29</accession>
    <name>Q1LG29_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Putative mechanosensitive ion channel</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">Rmet_4029</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="11">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="11">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MscS (TC 1.A.23) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10897.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518536.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4029"/>
    <dbReference type="EnsemblBacteria" id="ABF10897">
        <property type="protein sequence ID" value="ABF10897"/>
        <property type="gene ID" value="Rmet_4029"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4029"/>
    <dbReference type="eggNOG" id="COG3264">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011796_1_0_4"/>
    <dbReference type="OMA" id="RATEIQM"/>
    <dbReference type="OrthoDB" id="1903165at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022249">
        <property type="entry name" value="DUF3772"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010920">
        <property type="entry name" value="LSM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006685">
        <property type="entry name" value="MscS_channel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011066">
        <property type="entry name" value="MscS_channel_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011014">
        <property type="entry name" value="MscS_channel_TM-2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023408">
        <property type="entry name" value="MscS_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12607">
        <property type="entry name" value="DUF3772"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00924">
        <property type="entry name" value="MS_channel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50182">
        <property type="entry name" value="SSF50182"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82689">
        <property type="entry name" value="SSF82689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82861">
        <property type="entry name" value="SSF82861"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0614">Plasmid</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="244"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="285"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="356"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="396"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="436"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="466"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="531"/>
            <end position="550"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="570"/>
            <end position="589"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="610"/>
            <end position="635"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="641"/>
            <end position="660"/>
        </location>
    </feature>
    <feature type="domain" description="DUF3772" evidence="9">
        <location>
            <begin position="167"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="153"/>
            <end position="190"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF12607"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ABF10897.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="832" mass="89753" checksum="D6CF5EEC5550D5B8" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="101" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LG39</accession>
    <name>Q1LG39_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">5'-nucleotidase SurE</fullName>
            <ecNumber evidence="5">3.1.3.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Nucleoside 5'-monophosphate phosphohydrolase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5 7" type="primary">surE</name>
        <name evidence="7" type="ordered locus">Rmet_4019</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="8">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="8">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Nucleotidase that shows phosphatase activity on nucleoside 5'-monophosphates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 5">
            <text>a ribonucleoside 5'-phosphate + H2O = a ribonucleoside + phosphate</text>
            <dbReference type="Rhea" id="RHEA:12484"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:18254"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58043"/>
            <dbReference type="EC" id="3.1.3.5"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
        <text evidence="5">Binds 1 divalent metal cation per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the SurE nucleotidase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.5" evidence="5"/>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10887.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518527.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4019"/>
    <dbReference type="EnsemblBacteria" id="ABF10887">
        <property type="protein sequence ID" value="ABF10887"/>
        <property type="gene ID" value="Rmet_4019"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4019"/>
    <dbReference type="eggNOG" id="COG0496">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045192_1_2_4"/>
    <dbReference type="OMA" id="MHVEERI"/>
    <dbReference type="OrthoDB" id="1909278at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106411">
        <property type="term" value="F:XMP 5'-nucleosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1210.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00060">
        <property type="entry name" value="SurE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030048">
        <property type="entry name" value="SurE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002828">
        <property type="entry name" value="SurE-like_Pase/nucleotidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036523">
        <property type="entry name" value="SurE-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30457">
        <property type="entry name" value="PTHR30457"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01975">
        <property type="entry name" value="SurE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64167">
        <property type="entry name" value="SSF64167"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00087">
        <property type="entry name" value="surE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 5 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0614">Plasmid</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SurE" evidence="6">
        <location>
            <begin position="9"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="14"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="15"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="46"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="99"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000815"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011062"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00060"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABF10887.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="259" mass="27598" checksum="92BABECAE3EC5083" modified="2006-05-30" version="1">MSEPVVERVLLTNDDGIDAPGLAVLAEVAATIAREVWIVAPEHDQSGTSHSISLHDPLRITEHGPRRFGISGTPGDCVVMAVRHVMRDTPPDLVLSGINRGGNLGLETVFSGTVGAAMTGMLLGIRSIALSQVFKDRNAVKWGTSRALAGDVIRRLVTAGWSDDACLNVNFPDVEADAAGPLTVSRQGVGLINAIDVRAHVDPRGFPYHWLQFSRGPRPDVDDAEAMVVARGAVSVTPLRFERTSEEAAVALADNLMRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="72" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LGL6</accession>
    <name>Q1LGL6_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_3842</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10710.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518356.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3842"/>
    <dbReference type="EnsemblBacteria" id="ABF10710">
        <property type="protein sequence ID" value="ABF10710"/>
        <property type="gene ID" value="Rmet_3842"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3842"/>
    <dbReference type="eggNOG" id="COG0572">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_103067_0_0_4"/>
    <dbReference type="OMA" id="IHVMGAS"/>
    <dbReference type="OrthoDB" id="1793376at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF10710.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="176" mass="19677" checksum="5F0D3E6F4D801FDF" modified="2006-05-30" version="1">MKILITGAAGSGTSTLANAIAEATQTHALETDDYFWRPTNPPYQVKFEPEERCARLLKDLRARPETVVAGAVMEWGEALEHAFDLVVFLYVPTPIRLARLKLREEHRFGKADTEFLEWAAQYDAGTAEGRSLEKHRQWLQNLRCPVLYLEGDGTVEARLQSVLAARNAIIEGAGPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-10-07" version="92" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LGP6</accession>
    <name>Q1LGP6_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Transcriptional regulator, LysR family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_3810</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the LysR transcriptional regulatory family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10680.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3810"/>
    <dbReference type="EnsemblBacteria" id="ABF10680">
        <property type="protein sequence ID" value="ABF10680"/>
        <property type="gene ID" value="Rmet_3810"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3810"/>
    <dbReference type="eggNOG" id="COG0583">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039613_6_0_4"/>
    <dbReference type="OMA" id="MRRHTDG"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005119">
        <property type="entry name" value="LysR_subst-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000847">
        <property type="entry name" value="Tscrpt_reg_HTH_LysR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00126">
        <property type="entry name" value="HTH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03466">
        <property type="entry name" value="LysR_substrate"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50931">
        <property type="entry name" value="HTH_LYSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH lysR-type" evidence="2">
        <location>
            <begin position="72"/>
            <end position="129"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF10680.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="372" mass="40671" checksum="80D61CDB5CC93191" modified="2006-05-30" version="1">MRLSPSGSVVTGNVFAMSPCHGASDHYRIDSAARASRKVLSPLRRIRTAMPEAPLAPRHRPAPWQQETTLRFELTDLRVFVAIAEAGSLTMGASDMHLTAPSASYRLKNLEQTVGVPLFNRTQKGMVLTAAGETMLQHARTILDNVEQLQIGMRRHTDGIEGHIRVYANSSTMCNLPVALSRFLAAYPNVNVDLEEHLSADSVRAVQEQRADIGLVAGAIDLHGLDSILYGADELLFVVPPRHPLGMRDSVSLVQALRHELVSIGSKSSNFLYLQDMATRAGIVPHVRVHAPNFPAVIRCVQEGAGIALVPRSVAAPAIAQGLVEGVVLDEPWAMREQRVVARSLQSLPDYTRAFIRFLTESSESFPDTVLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="108" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LH02</accession>
    <name>Q1LH02_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">RNA polymerase sigma factor FliA</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">RNA polymerase sigma factor for flagellar operon</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="5">Sigma F</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="5">Sigma-28</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5 7" type="primary">fliA</name>
        <name evidence="7" type="ordered locus">Rmet_3702</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="8">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="8">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor controls the expression of flagella-related genes.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the sigma-70 factor family. FliA subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10574.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008641079.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3702"/>
    <dbReference type="EnsemblBacteria" id="ABF10574">
        <property type="protein sequence ID" value="ABF10574"/>
        <property type="gene ID" value="Rmet_3702"/>
    </dbReference>
    <dbReference type="GeneID" id="60826257"/>
    <dbReference type="KEGG" id="rme:Rmet_3702"/>
    <dbReference type="eggNOG" id="COG1191">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014793_8_1_4"/>
    <dbReference type="OMA" id="IKFETYA"/>
    <dbReference type="OrthoDB" id="1857862at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed 5'-3' RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016987">
        <property type="term" value="F:sigma factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006352">
        <property type="term" value="P:DNA-templated transcription, initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00962">
        <property type="entry name" value="Sigma70_FliA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014284">
        <property type="entry name" value="RNA_pol_sigma-70_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000943">
        <property type="entry name" value="RNA_pol_sigma70"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007627">
        <property type="entry name" value="RNA_pol_sigma70_r2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007624">
        <property type="entry name" value="RNA_pol_sigma70_r3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007630">
        <property type="entry name" value="RNA_pol_sigma70_r4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012845">
        <property type="entry name" value="RNA_pol_sigma_FliA_WhiG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013325">
        <property type="entry name" value="RNA_pol_sigma_r2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013324">
        <property type="entry name" value="RNA_pol_sigma_r3/r4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028617">
        <property type="entry name" value="Sigma70_FliA"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04542">
        <property type="entry name" value="Sigma70_r2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04539">
        <property type="entry name" value="Sigma70_r3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04545">
        <property type="entry name" value="Sigma70_r4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000770">
        <property type="entry name" value="RNA_pol_sigma-SigE/K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00046">
        <property type="entry name" value="SIGMA70FCT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88659">
        <property type="entry name" value="SSF88659"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88946">
        <property type="entry name" value="SSF88946"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02479">
        <property type="entry name" value="FliA_WhiG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02937">
        <property type="entry name" value="sigma70-ECF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00715">
        <property type="entry name" value="SIGMA70_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-0614">Plasmid</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0731">Sigma factor</keyword>
    <keyword evidence="4 5" id="KW-0804">Transcription</keyword>
    <keyword evidence="1 5" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="RNA_pol_sigma70" evidence="6">
        <location>
            <begin position="40"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="H-T-H motif" evidence="5">
        <location>
            <begin position="205"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="region of interest" description="Sigma-70 factor domain-2" evidence="5">
        <location>
            <begin position="13"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="region of interest" description="Sigma-70 factor domain-4" evidence="5">
        <location>
            <begin position="183"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Interaction with polymerase core subunit RpoC" evidence="5">
        <location>
            <begin position="40"/>
            <end position="43"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS00715"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABF10574.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="238" mass="27344" checksum="72085696E65509EA" modified="2006-05-30" version="1">MYTIQGKLEQADVVKSHAPLVRRIALQLAAKLPASVQIDDLIQAGMIGLLDAAKRYEDNHGARFETYASQRIRGAMLDEVRANDWQSRSLRQFTRKIERTQRQLEQKLGRSPIDSEVAKELEMGLEEYQELLNEVHGCQLLHYEDFERSGEEDFLDRHLGVTDEANPLTVLMESGMRDALIRAIEKLPEREKLVLSLCYDQELNLREIGAVLEVTESRVCQIRSQAITRLRNQLRGML</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LH03</accession>
    <name>Q1LH03_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Flagellar biosynthesis protein FlhG</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">flhG</name>
        <name evidence="2" type="ordered locus">Rmet_3701</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10573.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3701"/>
    <dbReference type="EnsemblBacteria" id="ABF10573">
        <property type="protein sequence ID" value="ABF10573"/>
        <property type="gene ID" value="Rmet_3701"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3701"/>
    <dbReference type="eggNOG" id="COG0455">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037612_0_0_4"/>
    <dbReference type="OMA" id="FKVAKNN"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002586">
        <property type="entry name" value="CobQ/CobB/MinD/ParA_Nub-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01656">
        <property type="entry name" value="CbiA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0966">Cell projection</keyword>
    <keyword evidence="2" id="KW-0969">Cilium</keyword>
    <keyword evidence="2" id="KW-0282">Flagellum</keyword>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CbiA" evidence="1">
        <location>
            <begin position="24"/>
            <end position="227"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF10573.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="274" mass="28341" checksum="366004CD804920C4" modified="2006-05-30" version="1">MNVMGSDQAESLRRLLAPRVTRRIAVVAADAGAGATTVTLGLANALAMQGERVLLVDEDRHGVRATRLSGAAPVATLADVLASRVSAAAAIGTRPASGIAVLPAGDIPVDAGNMDRALSDLIPDMRSVLIDARTDATGALSPLAATAHNVVIVMRPEVTSITAAYARIKRLHHEYACRRFHLVVNMAATEATVAALARNLARTASQYLGVEAGLTGYLPIDPLVARAFQLSRCVVEAYPAAASTSALRHIASGIGAWPLRHDTTPQGAAVAVPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LH45</accession>
    <name>Q1LH45_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Flp pilus assembly protein (TPR domain), transmembrane</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">tadD</name>
        <name evidence="4" type="ordered locus">Rmet_3659</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="5">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="5">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10531.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518186.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3659"/>
    <dbReference type="EnsemblBacteria" id="ABF10531">
        <property type="protein sequence ID" value="ABF10531"/>
        <property type="gene ID" value="Rmet_3659"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3659"/>
    <dbReference type="eggNOG" id="COG0457">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069566_0_0_4"/>
    <dbReference type="OMA" id="WYASLAH"/>
    <dbReference type="OrthoDB" id="1936152at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016931">
        <property type="entry name" value="UCP029658_TPR"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF029658">
        <property type="entry name" value="UCP029658_TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="SSF48452"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0614">Plasmid</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0802">TPR repeat</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193451" evidence="3">
        <location>
            <begin position="42"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="1">
        <location>
            <begin position="136"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="250"/>
            <end position="273"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF10531.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="273" mass="29574" checksum="9127382AF434506A" modified="2006-05-30" version="1">MKTVMSPWSRWSPRTLLPASLAMPLAAALLLSGCASTPNTAEVMAQQADAQIELAKLRDKEARAEYNDQSVYLGLINRMQTEGMYFASLAHIDAFQQKFGSNPSLLAMRADALRETGQDDAALQAYRDLLGTDRAARAHHGIGLVLGRQGDFMRAAGELRQAVSMEPVNPQYSSDLGYALMRGGALQDARVPVMQALELDAANPRLISNAVVWLWASGKRAEANAMMQRAAMQEPTRAAIRKEADRVSRAAQVRDKTAPRGAQAVALNAGVKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-12-02" version="101" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LH65</accession>
    <name>Q1LH65_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Transcriptional regulator, IclR family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Rmet_3639</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="6">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="6">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10511.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3639"/>
    <dbReference type="EnsemblBacteria" id="ABF10511">
        <property type="protein sequence ID" value="ABF10511"/>
        <property type="gene ID" value="Rmet_3639"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3639"/>
    <dbReference type="eggNOG" id="COG1414">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_062618_0_1_4"/>
    <dbReference type="OMA" id="HTHKIMS"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046278">
        <property type="term" value="P:3,4-dihydroxybenzoate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045893">
        <property type="term" value="P:positive regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029016">
        <property type="entry name" value="GAF-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012794">
        <property type="entry name" value="PcaR_PcaU"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014757">
        <property type="entry name" value="Tscrpt_reg_IclR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005471">
        <property type="entry name" value="Tscrpt_reg_IclR_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09339">
        <property type="entry name" value="HTH_IclR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01614">
        <property type="entry name" value="IclR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00346">
        <property type="entry name" value="HTH_ICLR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02431">
        <property type="entry name" value="pcaR_pcaU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51077">
        <property type="entry name" value="HTH_ICLR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51078">
        <property type="entry name" value="ICLR_ED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0614">Plasmid</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH iclR-type" evidence="3">
        <location>
            <begin position="56"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="domain" description="IclR-ED" evidence="4">
        <location>
            <begin position="117"/>
            <end position="301"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABF10511.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="303" mass="32851" checksum="055AB5A246C8B61E" modified="2006-05-30" version="1">MRQTGKAGAFPLPFRPFRANSGASTPMFRSMANPADTATSRAPVAGATEKPSDSYVQSFARGLSVIRAFNASHPAQTLTEIAQASGLTRAGARRILLTLVGLGYVQNDGRLFRLTPKILDLGFAYLTSMPFWNLAEPIMEALSQSVHESCSISVLDGTEIVYVLRVPARKIMTINLSIGSRLPAYCSSMGRVLLSGLDQEELDRVLHASDLRPRTRRTVTDIGELKTLIGEIRERGWAQNDQELEEGLVSLSAPIRNRGGQVIAAINISGQANRTSAQEMVERFLPPLLEAAGKISAMVGLRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LH66</accession>
    <name>Q1LH66_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Ferredoxin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_3638</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10510.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3638"/>
    <dbReference type="EnsemblBacteria" id="ABF10510">
        <property type="protein sequence ID" value="ABF10510"/>
        <property type="gene ID" value="Rmet_3638"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3638"/>
    <dbReference type="eggNOG" id="COG2871">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_082632_8_0_4"/>
    <dbReference type="OMA" id="TCKCRIE"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00207">
        <property type="entry name" value="fer2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036010">
        <property type="entry name" value="2Fe-2S_ferredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001041">
        <property type="entry name" value="2Fe-2S_ferredoxin-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006058">
        <property type="entry name" value="2Fe2S_fd_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012675">
        <property type="entry name" value="Beta-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00111">
        <property type="entry name" value="Fer2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54292">
        <property type="entry name" value="SSF54292"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00197">
        <property type="entry name" value="2FE2S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51085">
        <property type="entry name" value="2FE2S_FER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="2Fe-2S ferredoxin-type" evidence="2">
        <location>
            <begin position="29"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF10510.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="119" mass="12933" checksum="65379044DCDF205D" modified="2006-05-30" version="1">MMQAQTDIGDIDAGAAVPDTDTPPKPRTIEITFVTNGEKTVNAPENSNLLRVSLREQGGIPFKCGGGICGTCKCRIERGLEHTDAVKPKERKHLSDDDLRAGYRMACQTFVKGDIAVSW</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="92" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LHP9</accession>
    <name>Q1LHP9_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Regulator of nucleoside diphosphate kinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">rnk</name>
        <name evidence="2" type="ordered locus">Rmet_3455</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10327.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517885.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3455"/>
    <dbReference type="EnsemblBacteria" id="ABF10327">
        <property type="protein sequence ID" value="ABF10327"/>
        <property type="gene ID" value="Rmet_3455"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3455"/>
    <dbReference type="eggNOG" id="COG0782">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_120358_1_1_4"/>
    <dbReference type="OMA" id="VHCREES"/>
    <dbReference type="OrthoDB" id="1536415at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070063">
        <property type="term" value="F:RNA polymerase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032784">
        <property type="term" value="P:regulation of DNA-templated transcription, elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.50.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036953">
        <property type="entry name" value="GreA/GreB_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018151">
        <property type="entry name" value="TF_GreA/GreB_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001437">
        <property type="entry name" value="Tscrpt_elong_fac_GreA/B_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023459">
        <property type="entry name" value="Tscrpt_elong_fac_GreA/B_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30437">
        <property type="entry name" value="PTHR30437"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01272">
        <property type="entry name" value="GreA_GreB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00830">
        <property type="entry name" value="GREAB_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="GreA_GreB" evidence="1">
        <location>
            <begin position="59"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF10327.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="145" mass="15513" checksum="2CEC7E4BD406ECF2" modified="2006-05-30" version="1">MAKTKNTTAAPSRPATLYLTELDVTRLEGIASRAGTAELDEMLDALLERAAIVSPDAIPKDVVTMNSRVVCALEGEAKPRDWTLVYPDDADLATGRLSVLSPIGQALLGARSGDTVDYRLPDGRAQRVTIVEIAFQPEASGQFTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LI93</accession>
    <name>Q1LI93_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">N-acetyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_3261</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10133.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517733.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3261"/>
    <dbReference type="EnsemblBacteria" id="ABF10133">
        <property type="protein sequence ID" value="ABF10133"/>
        <property type="gene ID" value="Rmet_3261"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3261"/>
    <dbReference type="eggNOG" id="COG0454">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_099842_0_0_4"/>
    <dbReference type="OMA" id="TLYLHDM"/>
    <dbReference type="OrthoDB" id="2055010at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008080">
        <property type="term" value="F:N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00583">
        <property type="entry name" value="Acetyltransf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="SSF55729"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="1">
        <location>
            <begin position="4"/>
            <end position="169"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF10133.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="169" mass="18080" checksum="88152BC39D4419AD" modified="2006-05-30" version="1">MPEIVLRPLAVADMPAVLAVQAQCYGDMLLESSDALASRLALSPDTCWAAALPDGALAAYLFTHPWPEDALPPLDGVLARDWMPGAALTWFVHDMAVAPVGRGMGLAQRLYAAARDAALADGLLSSRLIAVQSAAVWWRRLGYATIAAGEYAEKLADYGDDAVLMERRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LIC9</accession>
    <name>Q1LIC9_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Rmet_3225</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10097.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3225"/>
    <dbReference type="EnsemblBacteria" id="ABF10097">
        <property type="protein sequence ID" value="ABF10097"/>
        <property type="gene ID" value="Rmet_3225"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3225"/>
    <dbReference type="eggNOG" id="COG3706">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017308_0_0_4"/>
    <dbReference type="OMA" id="MIEIDIT"/>
    <dbReference type="OrthoDB" id="1635706at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01949">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000160">
        <property type="entry name" value="GGDEF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003660">
        <property type="entry name" value="HAMP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029787">
        <property type="entry name" value="Nucleotide_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00990">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00267">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55073">
        <property type="entry name" value="SSF55073"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00254">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50887">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50885">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="24"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="331"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="domain" description="HAMP" evidence="3">
        <location>
            <begin position="359"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="domain" description="GGDEF" evidence="4">
        <location>
            <begin position="459"/>
            <end position="595"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="404"/>
            <end position="431"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABF10097.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="599" mass="65682" checksum="A9BACBED7E12D9D3" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LJ75</accession>
    <name>Q1LJ75_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Glycolate oxidase FAD binding subunit</fullName>
            <ecNumber evidence="2">1.1.3.15</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">glcE</name>
        <name evidence="2" type="ordered locus">Rmet_2928</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EC" id="1.1.3.15" evidence="2"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09801.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517473.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2928"/>
    <dbReference type="EnsemblBacteria" id="ABF09801">
        <property type="protein sequence ID" value="ABF09801"/>
        <property type="gene ID" value="Rmet_2928"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2928"/>
    <dbReference type="eggNOG" id="COG0277">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017779_0_0_4"/>
    <dbReference type="OMA" id="PLNASCW"/>
    <dbReference type="OrthoDB" id="295054at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003973">
        <property type="term" value="F:(S)-2-hydroxy-acid oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.465.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016166">
        <property type="entry name" value="FAD-bd_PCMH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036318">
        <property type="entry name" value="FAD-bd_PCMH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016169">
        <property type="entry name" value="FAD-bd_PCMH_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016164">
        <property type="entry name" value="FAD-linked_Oxase-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006094">
        <property type="entry name" value="Oxid_FAD_bind_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01565">
        <property type="entry name" value="FAD_binding_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55103">
        <property type="entry name" value="SSF55103"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56176">
        <property type="entry name" value="SSF56176"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51387">
        <property type="entry name" value="FAD_PCMH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD-binding PCMH-type" evidence="1">
        <location>
            <begin position="1"/>
            <end position="175"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF09801.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="367" mass="39032" checksum="82D02235FBF984E3" modified="2006-05-30" version="1">MQATLDAFRDAIRQAGDTRTPLRLRGGGSKDFYGQALAGTVLDTRAYRGIVEYDPAELVITARCGTPLAEIEDAVAQKRQMLAFEPPHFALRGQASTATIGGAVAAGLSGPRRQSAGALRDFVLGAQLMDGHGEIMNFGGQVMKNVAGYDVSRLLAGSLGTLGLILEVSLKVLPVPFDDATLRFAMDQAEAIDQLNKWGGQPLPIAASAWHDGFLHVRLSGATAAVCAARDNLGGEVVDASHAQQLWMVLREQTHAFFSPANDTGRALWRIAVPTVCKPLDLPGAQLVEWGGGQRWWIAPDDSAASADRVRAAAQAAGGHATLFRNGDKSVGVFTPLSSPLAAIHKRLKDTFDPAGIFNPQRMYNGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LJ91</accession>
    <name>Q1LJ91_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">ATPase regulatory factor involved in DnaA inactivation</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">hda</name>
        <name evidence="2" type="ordered locus">Rmet_2912</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09785.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517458.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2912"/>
    <dbReference type="EnsemblBacteria" id="ABF09785">
        <property type="protein sequence ID" value="ABF09785"/>
        <property type="gene ID" value="Rmet_2912"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2912"/>
    <dbReference type="eggNOG" id="COG0593">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_072265_1_0_4"/>
    <dbReference type="OMA" id="DWGQIYR"/>
    <dbReference type="OrthoDB" id="1110566at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032297">
        <property type="term" value="P:negative regulation of DNA-templated DNA replication initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013317">
        <property type="entry name" value="DnaA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017788">
        <property type="entry name" value="Hda"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00308">
        <property type="entry name" value="Bac_DnaA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03420">
        <property type="entry name" value="DnaA_homol_Hda"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Bac_DnaA" evidence="1">
        <location>
            <begin position="19"/>
            <end position="212"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF09785.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="235" mass="26698" checksum="E596519DB4F4F27C" modified="2006-05-30" version="1">MSPRQKQLSLELGSPPPSTFENFFVAANRESVQRLRELPGLVAREQATDRLIYLWGEIGSGRTHLLHAVCDAALHTGLNCRYLSPHHALSDFMFDPSCQIYTVDDVELLDEARQIAVFSLYNEVRAHVRCALVVAGGLAPRAMPVREDLRTRLGWGLVYQVESLSDEDKMAALLQAARERGLQLSPEIPHWLVTRHYRDMPSLMALLDALDTYSLERKRAVTLPLLREMFAEFRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="62" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LJI0</accession>
    <name>Q1LJI0_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_2823</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09696.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517389.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF09696">
        <property type="protein sequence ID" value="ABF09696"/>
        <property type="gene ID" value="Rmet_2823"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2823"/>
    <dbReference type="eggNOG" id="COG1403">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_149308_0_1_4"/>
    <dbReference type="OMA" id="CIPCHKQ"/>
    <dbReference type="OrthoDB" id="1671839at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd00085">
        <property type="entry name" value="HNHc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003615">
        <property type="entry name" value="HNH_nuc"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF09696.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="120" mass="13501" checksum="634239483EF5D8BC" modified="2006-05-30" version="1">MVVKRRLREGRDGQLGPPPEAPTATGSLCPLCGRRLLDDATTDLHHPVPRSHGGRAVVPMHRVCHQKIHSVFTEAELATTYHDWNALRAHPAIADFIRWIARKPPGFYDRSRATNARRGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="61" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LJN8</accession>
    <name>Q1LJN8_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_2765</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09638.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517336.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2765"/>
    <dbReference type="EnsemblBacteria" id="ABF09638">
        <property type="protein sequence ID" value="ABF09638"/>
        <property type="gene ID" value="Rmet_2765"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2765"/>
    <dbReference type="eggNOG" id="ENOG5032ZGA">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_102164_1_0_4"/>
    <dbReference type="OMA" id="DTYIDDY"/>
    <dbReference type="OrthoDB" id="1944349at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021735">
        <property type="entry name" value="DUF3306"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11748">
        <property type="entry name" value="DUF3306"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="143"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="35"/>
            <end position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF09638.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="202" mass="21365" checksum="895447CC90932F10" modified="2006-05-30" version="1">MSDGDSSFLSRWSRRKAAVREGKPVPEEQVQEQVQPPAVLPSSTATSPDVTAAVPTPAASAVPMPTLADVAVLRPGDNIARFVAQGVDESVKRAALKTLFADPHFNVMDGLDTYIDDYSKPDPIPPEILRRLRQSETLGLFDPVEEAEDVAEVEEAGKAGKAVDDAAASQAPQPGQGLQAAMVEEPTASSEPDEDPAADKHA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-08-12" version="69" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LK39</accession>
    <name>Q1LK39_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Putative oxidoreductase with FAD/NAD(P)-binding domain</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">yhiN</name>
        <name evidence="1" type="ordered locus">Rmet_2610</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09487.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2610"/>
    <dbReference type="EnsemblBacteria" id="ABF09487">
        <property type="protein sequence ID" value="ABF09487"/>
        <property type="gene ID" value="Rmet_2610"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2610"/>
    <dbReference type="eggNOG" id="COG2081">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025174_2_0_4"/>
    <dbReference type="OMA" id="RCNFTNM"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.260">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004792">
        <property type="entry name" value="BaiN-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023166">
        <property type="entry name" value="HI0933-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42887">
        <property type="entry name" value="PTHR42887"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="SSF51905"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00275">
        <property type="entry name" value="TIGR00275"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF09487.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="378" mass="40794" checksum="79EFD1674B97553B" modified="2006-05-30" version="1">MIDHATKLAEKIRISGGGRCNFTNLQASPANYLSSNPHFCRSALARYTPQDFLALMRRYDIGWHEKHRGQLFCNDSAEDVINLLRAECEAGGVRWQTGCSIHEVRREGADYLLLTSQGTVRVGALVVATGGLSIPKIGATDFGYAIARQFGLKIIDTRPALVPLTFDGQAWQPFVPLAGVSMEVDISTGSGKTAGAFKEDLLWTHRGLSGPAVLQISSYWRPGTPIEIDLFDGEDAAAWLIGHKKSSRKHLNNLLGERLPSRLADAWCAAVGLQGTQSVADMPDKALRQLGESLNRWRLVPSGTEGYKKAEVTLGGVDTRGLSSTTMMAREVDGLYFIGEVVDVTGWLGGYNFQWAWASAVAAGEAVAEAARGAGAVT</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LK51</accession>
    <name>Q1LK51_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Pimeloyl-CoA synthetase</fullName>
            <ecNumber evidence="3">6.2.1.14</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">pauA</name>
        <name evidence="3" type="ordered locus">Rmet_2598</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EC" id="6.2.1.14" evidence="3"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09475.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517178.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2598"/>
    <dbReference type="EnsemblBacteria" id="ABF09475">
        <property type="protein sequence ID" value="ABF09475"/>
        <property type="gene ID" value="Rmet_2598"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2598"/>
    <dbReference type="eggNOG" id="COG1042">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007415_3_1_4"/>
    <dbReference type="OMA" id="ASHTGKL"/>
    <dbReference type="OrthoDB" id="445933at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042410">
        <property type="term" value="F:6-carboxyhexanoate-CoA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.261">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011761">
        <property type="entry name" value="ATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013815">
        <property type="entry name" value="ATP_grasp_subdomain_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003781">
        <property type="entry name" value="CoA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032875">
        <property type="entry name" value="Succ_CoA_lig_flav_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016102">
        <property type="entry name" value="Succinyl-CoA_synth-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13380">
        <property type="entry name" value="CoA_binding_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13607">
        <property type="entry name" value="Succ_CoA_lig"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00881">
        <property type="entry name" value="CoA_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52210">
        <property type="entry name" value="SSF52210"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50975">
        <property type="entry name" value="ATP_GRASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0436">Ligase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ATP-grasp" evidence="2">
        <location>
            <begin position="492"/>
            <end position="528"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF09475.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="708" mass="74942" checksum="1AAF1BE742F3877C" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LK77</accession>
    <name>Q1LK77_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Molybdochelatase MogA, involved in Moco biosynthesis</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">mogA</name>
        <name evidence="4" type="ordered locus">Rmet_2572</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; molybdopterin biosynthesis.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09449.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517152.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2572"/>
    <dbReference type="EnsemblBacteria" id="ABF09449">
        <property type="protein sequence ID" value="ABF09449"/>
        <property type="gene ID" value="Rmet_2572"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2572"/>
    <dbReference type="eggNOG" id="COG0521">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_077358_1_0_4"/>
    <dbReference type="OMA" id="EYLTSEW"/>
    <dbReference type="OrthoDB" id="1877633at2"/>
    <dbReference type="UniPathway" id="UPA00344"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006777">
        <property type="term" value="P:Mo-molybdopterin cofactor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00886">
        <property type="entry name" value="MogA_MoaB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.980.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036425">
        <property type="entry name" value="MoaB/Mog-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001453">
        <property type="entry name" value="MoaB/Mog_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008284">
        <property type="entry name" value="MoCF_biosynth_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00994">
        <property type="entry name" value="MoCF_biosynth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00852">
        <property type="entry name" value="MoCF_biosynth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53218">
        <property type="entry name" value="SSF53218"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00177">
        <property type="entry name" value="molyb_syn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01078">
        <property type="entry name" value="MOCF_BIOSYNTHESIS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0501">Molybdenum cofactor biosynthesis</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MoCF_biosynth" evidence="3">
        <location>
            <begin position="22"/>
            <end position="172"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005046"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF09449.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="216" mass="23244" checksum="9180BBE0F4B8093D" modified="2006-05-30" version="1">MNPQTPPTSPVARKSPDELVVGFVSISDRASSGTYQDEGIPALRDWFGKTLTTPWQSVERLIPDEQAQISRTLIELVDVAGCDLVLTTGGTGPARRDVTPEATLAVGTKEMPGFGEQMRQVSLHFVPTAILSRQVAVIRETPSRAALIVNLPGQPRAIRETLEGLRDADGKSIVQGVFAAMPYCIDLIGGPYIETDEAVVKAWRPKNAVRAKPASA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LKE6</accession>
    <name>Q1LKE6_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Gluconate 2-dehydrogenase flavoprotein (GADH dehydrogenase subunit) (GA 2-DH dehydrogenase subunit)</fullName>
            <ecNumber evidence="3">1.1.99.3</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_2503</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EC" id="1.1.99.3" evidence="3"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09380.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517081.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2503"/>
    <dbReference type="EnsemblBacteria" id="ABF09380">
        <property type="protein sequence ID" value="ABF09380"/>
        <property type="gene ID" value="Rmet_2503"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2503"/>
    <dbReference type="eggNOG" id="COG2303">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008878_2_0_4"/>
    <dbReference type="OMA" id="PGYYIHE"/>
    <dbReference type="OrthoDB" id="365211at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033717">
        <property type="term" value="F:gluconate 2-dehydrogenase (acceptor) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000172">
        <property type="entry name" value="GMC_OxRdtase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007867">
        <property type="entry name" value="GMC_OxRtase_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05199">
        <property type="entry name" value="GMC_oxred_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00732">
        <property type="entry name" value="GMC_oxred_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="SSF51905"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GMC_OxRdtase_N" evidence="1">
        <location>
            <begin position="202"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="domain" description="GMC_oxred_C" evidence="2">
        <location>
            <begin position="444"/>
            <end position="572"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00732"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF05199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF09380.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="591" mass="64966" checksum="913429E578DE8EBC" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LKJ8</accession>
    <name>Q1LKJ8_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Ferredoxin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">fdxA</name>
        <name evidence="9" type="ordered locus">Rmet_2451</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Ferredoxins are iron-sulfur proteins that transfer electrons in a wide variety of metabolic reactions.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 7">
            <name>[3Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:21137"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 7">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09328.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008648407.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2451"/>
    <dbReference type="EnsemblBacteria" id="ABF09328">
        <property type="protein sequence ID" value="ABF09328"/>
        <property type="gene ID" value="Rmet_2451"/>
    </dbReference>
    <dbReference type="GeneID" id="60821075"/>
    <dbReference type="KEGG" id="rme:Rmet_2451"/>
    <dbReference type="eggNOG" id="COG1146">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_139698_0_0_4"/>
    <dbReference type="OMA" id="DRMLYIH"/>
    <dbReference type="OrthoDB" id="1873445at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051538">
        <property type="term" value="F:3 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000813">
        <property type="entry name" value="7Fe_ferredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022569">
        <property type="entry name" value="Fd_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11953">
        <property type="entry name" value="DUF3470"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00037">
        <property type="entry name" value="Fer4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12800">
        <property type="entry name" value="Fer4_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00354">
        <property type="entry name" value="7FE8SFRDOXIN"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 7" id="KW-0003">3Fe-4S</keyword>
    <keyword evidence="7" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="7" id="KW-0249">Electron transport</keyword>
    <keyword evidence="4 7" id="KW-0408">Iron</keyword>
    <keyword evidence="5 7" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="3 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0813">Transport</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="8">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="8">
        <location>
            <begin position="31"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU364098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ABF09328.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="107" mass="12118" checksum="1817B3D221D9B622" modified="2006-05-30" version="1">MTHVVTESCIRCRYTDCVDVCPVDCFREGPNFLSIDPDECIDCAVCVAECPVNAIYAEEDVPGDQQQFIELNAELSRIWPSITKTKAPLAEAEEWKDATDKLQYLQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="103" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LKL9</accession>
    <name>Q1LKL9_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">3-oxoacyl-[acyl-carrier-protein] synthase 3</fullName>
            <ecNumber evidence="8">2.3.1.180</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">3-oxoacyl-[acyl-carrier-protein] synthase III</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">Beta-ketoacyl-ACP synthase III</fullName>
            <shortName evidence="8">KAS III</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8 11" type="primary">fabH</name>
        <name evidence="11" type="ordered locus">Rmet_2430</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Catalyzes the condensation reaction of fatty acid synthesis by the addition to an acyl acceptor of two carbons from malonyl-ACP. Catalyzes the first condensation reaction which initiates fatty acid synthesis and may therefore play a role in governing the total rate of fatty acid production. Possesses both acetoacetyl-ACP synthase and acetyl transacylase activities. Its substrate specificity determines the biosynthesis of branched-chain and/or straight-chain of fatty acids.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>acetyl-CoA + H(+) + malonyl-[ACP] = 3-oxobutanoyl-[ACP] + CO2 + CoA</text>
            <dbReference type="Rhea" id="RHEA:12080"/>
            <dbReference type="Rhea" id="RHEA-COMP:9623"/>
            <dbReference type="Rhea" id="RHEA-COMP:9625"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:78449"/>
            <dbReference type="ChEBI" id="CHEBI:78450"/>
            <dbReference type="EC" id="2.3.1.180"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="8">Lipid metabolism; fatty acid biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="8">The last Arg residue of the ACP-binding site is essential for the weak association between ACP/AcpP and FabH.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the thiolase-like superfamily. FabH family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.180" evidence="8"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09307.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2430"/>
    <dbReference type="EnsemblBacteria" id="ABF09307">
        <property type="protein sequence ID" value="ABF09307"/>
        <property type="gene ID" value="Rmet_2430"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2430"/>
    <dbReference type="eggNOG" id="COG0332">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039592_3_1_4"/>
    <dbReference type="OMA" id="WGSEGDK"/>
    <dbReference type="UniPathway" id="UPA00094"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004315">
        <property type="term" value="F:3-oxoacyl-[acyl-carrier-protein] synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033818">
        <property type="term" value="F:beta-ketoacyl-acyl-carrier-protein synthase III activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006633">
        <property type="term" value="P:fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.47.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01815">
        <property type="entry name" value="FabH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013751">
        <property type="entry name" value="ACP_syn_III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013747">
        <property type="entry name" value="ACP_syn_III_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004655">
        <property type="entry name" value="FabH_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016039">
        <property type="entry name" value="Thiolase-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08545">
        <property type="entry name" value="ACP_syn_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08541">
        <property type="entry name" value="ACP_syn_III_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53901">
        <property type="entry name" value="SSF53901"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00747">
        <property type="entry name" value="fabH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8 11" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 8" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="4 8" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="2 8" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="5 8" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="8" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8 11" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="ACP_syn_III" evidence="10">
        <location>
            <begin position="138"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="domain" description="ACP_syn_III_C" evidence="9">
        <location>
            <begin position="262"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="region of interest" description="ACP-binding" evidence="8">
        <location>
            <begin position="278"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="active site" evidence="8">
        <location>
            <position position="144"/>
        </location>
    </feature>
    <feature type="active site" evidence="8">
        <location>
            <position position="277"/>
        </location>
    </feature>
    <feature type="active site" evidence="8">
        <location>
            <position position="307"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01815"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF08541"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF08545"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ABF09307.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="350" mass="36942" checksum="CCF3F2D6B4D986CC" modified="2006-05-30" version="1">MPAAAGSLRDRAQGDPVSITIRHDMTKYAKIIGTGSYLPPRRVTNADLAAQLAEKGIETSDEWIVSRSGISARYWAEPDVTSSDLAVKAAERAIEAAGIDRQDIDLIIVATSTPDYVFPSAACLVQQKLGITNQCAAFDLQAVCSGFVYALATADKFIRSGSHKNVLVIGSEVFSRILDFNDRTTCVLFGDGAGAVVLTASDEPGILSTAMHSDGSHVGILCVPGNVSGGAITGNAFLHMDGQAVFKLAVTVLDKVAREAITAAEMQADEIDWLIPHQANIRIMQGTAKKLGLPSERMIATVHEHGNTSAASIPLALDVAVRDGRIKPGQTVLMEGVGGGFTWGAVLLRM</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="59" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LKP7</accession>
    <name>Q1LKP7_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">traR</name>
        <name evidence="2" type="ordered locus">Rmet_2402</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09279.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_003149848.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2402"/>
    <dbReference type="EnsemblBacteria" id="ABF09279">
        <property type="protein sequence ID" value="ABF09279"/>
        <property type="gene ID" value="Rmet_2402"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2402"/>
    <dbReference type="eggNOG" id="COG0583">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039613_11_0_4"/>
    <dbReference type="OMA" id="DYPIYRW"/>
    <dbReference type="OrthoDB" id="1322102at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005119">
        <property type="entry name" value="LysR_subst-bd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03466">
        <property type="entry name" value="LysR_substrate"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="LysR_substrate" evidence="1">
        <location>
            <begin position="10"/>
            <end position="200"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF09279.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="218" mass="23954" checksum="AD2DC62276ACD64B" modified="2006-05-30" version="1">MHDATSQFPHLRLAIAPGVPSSCFSSLLALQRAEEPEITIAFFETSSDDLVTGIDEGRYDAGMSLRAVSAPSLKSQPLWIENIAVAMPLWSPLLAQTKLTIAELLDYPVFRWPAESCSLLDQRLSSLPSLSRQSVQHVTSFDMLALWVGAGYGVGITAQSRIERADAWGITARPLAEGPYEVVTHLQRLKGRTNAVSERFERRAMQVAKDSAVQSNTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="101" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LKZ5</accession>
    <name>Q1LKZ5_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Transcriptional regulator, MerR-family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">pbrR2</name>
        <name evidence="6" type="ordered locus">Rmet_2302</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="journal article" date="2016" name="Inorg. Chem." volume="55" first="12516" last="12519">
            <title>Structural Basis for the Selective Pb(II) Recognition of Metalloregulatory Protein PbrR691.</title>
            <authorList>
                <person name="Huang S."/>
                <person name="Liu X."/>
                <person name="Wang D."/>
                <person name="Chen W."/>
                <person name="Hu Q."/>
                <person name="Wei T."/>
                <person name="Zhou W."/>
                <person name="Gan J."/>
                <person name="Chen H."/>
            </authorList>
            <dbReference type="PubMed" id="27989185"/>
            <dbReference type="DOI" id="10.1021/acs.inorgchem.6b02397"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (2.01 ANGSTROMS) OF 1-129</scope>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09181.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_003098965.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="PDB" id="5GPE">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.01 A"/>
        <property type="chains" value="A/B/C/D/E/F/G/H=1-129"/>
    </dbReference>
    <dbReference type="PDBsum" id="5GPE"/>
    <dbReference type="SMR" id="Q1LKZ5"/>
    <dbReference type="STRING" id="266264.Rmet_2302"/>
    <dbReference type="EnsemblBacteria" id="ABF09181">
        <property type="protein sequence ID" value="ABF09181"/>
        <property type="gene ID" value="Rmet_2302"/>
    </dbReference>
    <dbReference type="GeneID" id="56409810"/>
    <dbReference type="GeneID" id="61526428"/>
    <dbReference type="KEGG" id="rme:Rmet_2302"/>
    <dbReference type="eggNOG" id="COG0789">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_060077_2_0_4"/>
    <dbReference type="OMA" id="HQSCASA"/>
    <dbReference type="OrthoDB" id="1748559at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045893">
        <property type="term" value="P:positive regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04784">
        <property type="entry name" value="HTH_CadR-PbrR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011791">
        <property type="entry name" value="CadR-PbrR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009061">
        <property type="entry name" value="DNA-bd_dom_put_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000551">
        <property type="entry name" value="MerR-type_HTH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015358">
        <property type="entry name" value="Tscrpt_reg_MerR_DNA-bd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00376">
        <property type="entry name" value="MerR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09278">
        <property type="entry name" value="MerR-DNA-bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00040">
        <property type="entry name" value="HTHMERR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00422">
        <property type="entry name" value="HTH_MERR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46955">
        <property type="entry name" value="SSF46955"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02047">
        <property type="entry name" value="CadR-PbrR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50937">
        <property type="entry name" value="HTH_MERR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword evidence="8" id="KW-0002">3D-structure</keyword>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH merR-type" evidence="5">
        <location>
            <begin position="1"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="85"/>
            <end position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABF09181.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="8">
        <source>
            <dbReference type="PDB" id="5GPE"/>
        </source>
    </evidence>
    <sequence length="132" mass="15231" checksum="71310F67AED673EA" modified="2006-05-30" version="1">MMRIGELGKKADCLVQTVRFYESEGLLPEPARSEGNFRLYDEVHLQRLLFIRRCRAKDMTLDEIRQLLNLRDRPELGCGEVNALVDAHIAQVRTKMKELRALERELMDLRRSCDSARTSRECGILNSLAEPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="66" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LL44</accession>
    <name>Q1LL44_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Probable protease involved in cellulose biosynthesis</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">bcsE</name>
        <name evidence="2" type="ordered locus">Rmet_2253</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09132.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516955.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2253"/>
    <dbReference type="EnsemblBacteria" id="ABF09132">
        <property type="protein sequence ID" value="ABF09132"/>
        <property type="gene ID" value="Rmet_2253"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2253"/>
    <dbReference type="eggNOG" id="ENOG502Z9EC">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_389181_0_0_4"/>
    <dbReference type="OMA" id="PAGWPRN"/>
    <dbReference type="OrthoDB" id="1259570at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035438">
        <property type="term" value="F:cyclic-di-GMP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008233">
        <property type="term" value="F:peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017745">
        <property type="entry name" value="BcsE"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10995">
        <property type="entry name" value="CBP_GIL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03369">
        <property type="entry name" value="cellulose_bcsE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="525"/>
            <end position="582"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="526"/>
            <end position="540"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF09132.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="582" mass="63484" checksum="F80814F6156F127E" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="59" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LL69</accession>
    <name>Q1LL69_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_2228</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09107.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516934.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2228"/>
    <dbReference type="EnsemblBacteria" id="ABF09107">
        <property type="protein sequence ID" value="ABF09107"/>
        <property type="gene ID" value="Rmet_2228"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2228"/>
    <dbReference type="eggNOG" id="COG1672">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_043775_1_0_4"/>
    <dbReference type="OrthoDB" id="1080661at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF09107.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="403" mass="44438" checksum="F366AB6D04E34847" modified="2006-05-30" version="1">MNESESIFRRRQLAAELADELMGDSRTGMAWSGLFLSAPRRTGKSTFLREDLQPALERRGALVLVADLWKNNDVDPGDVIVDVVRQALAAHEGVVARAARAVGLDRFGFGGFSFSIDRVGLGKGVALSDALAELSDKVQRPIALIIDEAQQCMATRRGNNAMFALKAARDELNFAHHGLRIIATGSDHLKLRMLLGSREAFFCATERIFPVLGRDYIDWFRSRHGFSDVLNADVMTHLFARAAYRPELFESAAVAVGRDGLVGDDAAAVLERRIDEQMRAVHAPAMRTLEELPPLDDAIIRVMATSKQMFAGFSASTMQTYRRVMNALSGEPSSRLDYAEVERALERLVVLRLVWRSALGLHVLEDPTLAEAMRRAGLLDVVRRVSGRKLTLVSGAEHDLSVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="68" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LL90</accession>
    <name>Q1LL90_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Methyltransferase-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_2207</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09086.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516914.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2207"/>
    <dbReference type="EnsemblBacteria" id="ABF09086">
        <property type="protein sequence ID" value="ABF09086"/>
        <property type="gene ID" value="Rmet_2207"/>
    </dbReference>
    <dbReference type="GeneID" id="60821220"/>
    <dbReference type="KEGG" id="rme:Rmet_2207"/>
    <dbReference type="eggNOG" id="COG2226">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_075049_0_0_4"/>
    <dbReference type="OMA" id="CEEQAWP"/>
    <dbReference type="OrthoDB" id="1730305at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013216">
        <property type="entry name" value="Methyltransf_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08241">
        <property type="entry name" value="Methyltransf_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Methyltransf_11" evidence="1">
        <location>
            <begin position="82"/>
            <end position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF08241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF09086.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="264" mass="29881" checksum="2761E016D9605DB0" modified="2006-05-30" version="1">MSNRPIIDWEEWLASPPGQYVLEWEGQQYDRTVADIFGYHAVQFGLPAIDTLRENRMPFSALALDGRAGPHGPRETHPDARQLLCRFDELPFDTQSIDLVTLPHVLEFSEDPHEVLREVSRVLMPEGRVVVTCFNPMSLWGARQGLNRLGAMPFLPSDAQTIGFVRIKDWLKLLGFEIIRGRFGCYCPPYRTERWLQRAAFMEKAGDRWWPIFGAVYMISAVKRVKNIRLVGPAWKTPKSALKPVAAPVATPTGTHGKTPGQDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LL97</accession>
    <name>Q1LL97_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Enoyl-CoA hydratase/isomerase</fullName>
            <ecNumber evidence="4">4.2.1.17</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Rmet_2200</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the enoyl-CoA hydratase/isomerase family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.17" evidence="4"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09079.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516907.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2200"/>
    <dbReference type="EnsemblBacteria" id="ABF09079">
        <property type="protein sequence ID" value="ABF09079"/>
        <property type="gene ID" value="Rmet_2200"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2200"/>
    <dbReference type="eggNOG" id="COG1024">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009834_7_2_4"/>
    <dbReference type="OMA" id="FESYAEL"/>
    <dbReference type="OrthoDB" id="1275789at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004300">
        <property type="term" value="F:enoyl-CoA hydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016853">
        <property type="term" value="F:isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.12.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029045">
        <property type="entry name" value="ClpP/crotonase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018376">
        <property type="entry name" value="Enoyl-CoA_hyd/isom_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001753">
        <property type="entry name" value="Enoyl-CoA_hydra/iso"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014748">
        <property type="entry name" value="Enoyl-CoA_hydra_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00378">
        <property type="entry name" value="ECH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52096">
        <property type="entry name" value="SSF52096"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00166">
        <property type="entry name" value="ENOYL_COA_HYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0413">Isomerase</keyword>
    <keyword evidence="4" id="KW-0456">Lyase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF09079.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="283" mass="30694" checksum="B09BE7C8797DC786" modified="2006-05-30" version="1">MSDIALDMRHHKRAFADYQPKHFLWSVSADGKVATITLNRPERKNPLTFDSYAELRDLFRSLCYATDIKTVVVTGAGGNFCSGGDVHEIIGPLTRMTMPELLDFTRMTGDLIKAMRACPQPIISAIDGICAGAGAMIALASDMRLGTALAKTAFLFVRVGLAGADMGACSLLPRVIGQGRASELLYTGRSMSAEEGLQWGFFNALHASETLLAEAQSLATQIAAGPTFAHGVTKKLLHQEWNMGLDEAIEAEAEAQAICMQTRDFRRAYDAFVAKQKPVFEGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="104" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LLB3</accession>
    <name>Q1LLB3_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Phosphate transport system permease protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">pstC</name>
        <name evidence="11" type="ordered locus">Rmet_2184</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Part of the binding-protein-dependent transport system for phosphate; probably responsible for the translocation of the substrate across the membrane.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cell inner membrane</location>
            <topology evidence="9">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="8">Cell membrane</location>
            <topology evidence="8">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the binding-protein-dependent transport system permease family. CysTW subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09063.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516891.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2184"/>
    <dbReference type="EnsemblBacteria" id="ABF09063">
        <property type="protein sequence ID" value="ABF09063"/>
        <property type="gene ID" value="Rmet_2184"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2184"/>
    <dbReference type="eggNOG" id="COG0573">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033621_1_3_4"/>
    <dbReference type="OMA" id="VIRMSVL"/>
    <dbReference type="OrthoDB" id="1325025at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005315">
        <property type="term" value="F:inorganic phosphate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006817">
        <property type="term" value="P:phosphate ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011864">
        <property type="entry name" value="Phosphate_PstC"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="SSF161098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02138">
        <property type="entry name" value="phosphate_pstC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0592">Phosphate transport</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 8" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="28"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="71"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="114"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="162"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="227"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="292"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="10">
        <location>
            <begin position="77"/>
            <end position="307"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU363054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ABF09063.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="321" mass="33886" checksum="73DC9858A78A3835" modified="2006-05-30" version="1">MATSSDLPVVRPPSRLGDILFGGLTRGAAIVTLLLLGGIIVSLAISAWPSISKFGISFLWNSEWDPPADVYGALVPVYGTIVTSLIALIIAVPVSFGIALFLTELSPAWLRRPLGTAIELLAAVPSIVYGMWGLLVFSPIFGEYFQKPLANTIGQVPVIGKLFQGAPLGIGLLCAGVILAIMIIPYIASVMRDVFEVTPVLLKESAYGIGCTTWEVMWRVVLPYTRAGVIGGVMLGLGRALGETMAVTFVIGNTNILDNVSLFSPGNSITSALANEFAEAGAGLHTAALMELGLILFFITFVVLALSKLLLLRLAKNEGGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LLB6</accession>
    <name>Q1LLB6_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Phosphate-specific transport system accessory protein PhoU</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">phoU</name>
        <name evidence="5" type="ordered locus">Rmet_2181</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Plays a role in the regulation of phosphate uptake.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the PhoU family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09060.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008641661.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2181"/>
    <dbReference type="EnsemblBacteria" id="ABF09060">
        <property type="protein sequence ID" value="ABF09060"/>
        <property type="gene ID" value="Rmet_2181"/>
    </dbReference>
    <dbReference type="GeneID" id="60821246"/>
    <dbReference type="KEGG" id="rme:Rmet_2181"/>
    <dbReference type="eggNOG" id="COG0704">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_078518_2_1_4"/>
    <dbReference type="OMA" id="WKHGIET"/>
    <dbReference type="OrthoDB" id="1648549at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030643">
        <property type="term" value="P:cellular phosphate ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045936">
        <property type="term" value="P:negative regulation of phosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006817">
        <property type="term" value="P:phosphate ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.220">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028366">
        <property type="entry name" value="P_transport_PhoU"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038078">
        <property type="entry name" value="PhoU-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026022">
        <property type="entry name" value="PhoU_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42930">
        <property type="entry name" value="PTHR42930"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01895">
        <property type="entry name" value="PhoU"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF003107">
        <property type="entry name" value="PhoU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02135">
        <property type="entry name" value="phoU_full"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 3" id="KW-0592">Phosphate transport</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="PhoU" evidence="4">
        <location>
            <begin position="21"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="domain" description="PhoU" evidence="4">
        <location>
            <begin position="130"/>
            <end position="210"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRNR" id="PIRNR003107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABF09060.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="234" mass="26202" checksum="715513E390B06A37" modified="2006-05-30" version="1">MTDKHLSTQFDADLNAINTKLLQMGGLVESQIELAMRALADFDGDLADQVITREQQLNALEVEIDADCGNIIARRQPTARDLRLVMAISKTITNLERAGDEAEKIAKRTKHIMEDAAAHTINYAEVKLSGEMAITLLRQALDAFARLDTVAAARIVKDDKAIDEEFRAFVRKLITYMMEDPRTISVALDFLFIAKAVERIGDHAKNIAEFIIYIVKGTDVRHASREDMEREALS</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-10-07" version="49" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LLC8</accession>
    <name>Q1LLC8_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_2169</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09048.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2169"/>
    <dbReference type="EnsemblBacteria" id="ABF09048">
        <property type="protein sequence ID" value="ABF09048"/>
        <property type="gene ID" value="Rmet_2169"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2169"/>
    <dbReference type="eggNOG" id="ENOG502ZD0I">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1282350_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF09048.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="215" mass="23162" checksum="BA8DB43A7CAC3775" modified="2006-05-30" version="1">MPFEGKHSGVGFIDCGPDEVMPLLQPISSVCAGFATTMLTAGGAYRGSICTPNQEGVCDATLLLVFVPLDWDMSPNRVDKSIDLTLDDFAAQVEEAELLSSQYLDLVSKYGAHLHVGQRLSGELPATSLALLFGVHISANAGQQMAERFRPFMTGPACSWAAEGEHRRLVRDGVLDDRHPTANQFASAIGCLPRSGMAKAKESSSIVSFEKFQNR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-09-02" modified="2022-08-03" version="99" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LLI8</accession>
    <name>RLMN_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Dual-specificity RNA methyltransferase RlmN</fullName>
            <ecNumber evidence="1">2.1.1.192</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">23S rRNA (adenine(2503)-C(2))-methyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">23S rRNA m2A2503 methyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Ribosomal RNA large subunit methyltransferase N</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">tRNA (adenine(37)-C(2))-methyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">tRNA m2A37 methyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rlmN</name>
        <name type="ordered locus">Rmet_2109</name>
    </gene>
    <organism>
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Specifically methylates position 2 of adenine 2503 in 23S rRNA and position 2 of adenine 37 in tRNAs. m2A2503 modification seems to play a crucial role in the proofreading step occurring at the peptidyl transferase center and thus would serve to optimize ribosomal fidelity.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>adenosine(2503) in 23S rRNA + 2 reduced [2Fe-2S]-[ferredoxin] + 2 S-adenosyl-L-methionine = 2-methyladenosine(2503) in 23S rRNA + 5'-deoxyadenosine + L-methionine + 2 oxidized [2Fe-2S]-[ferredoxin] + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:42916"/>
            <dbReference type="Rhea" id="RHEA-COMP:10000"/>
            <dbReference type="Rhea" id="RHEA-COMP:10001"/>
            <dbReference type="Rhea" id="RHEA-COMP:10152"/>
            <dbReference type="Rhea" id="RHEA-COMP:10282"/>
            <dbReference type="ChEBI" id="CHEBI:17319"/>
            <dbReference type="ChEBI" id="CHEBI:33737"/>
            <dbReference type="ChEBI" id="CHEBI:33738"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:74411"/>
            <dbReference type="ChEBI" id="CHEBI:74497"/>
            <dbReference type="EC" id="2.1.1.192"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>adenosine(37) in tRNA + 2 reduced [2Fe-2S]-[ferredoxin] + 2 S-adenosyl-L-methionine = 2-methyladenosine(37) in tRNA + 5'-deoxyadenosine + L-methionine + 2 oxidized [2Fe-2S]-[ferredoxin] + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:43332"/>
            <dbReference type="Rhea" id="RHEA-COMP:10000"/>
            <dbReference type="Rhea" id="RHEA-COMP:10001"/>
            <dbReference type="Rhea" id="RHEA-COMP:10162"/>
            <dbReference type="Rhea" id="RHEA-COMP:10485"/>
            <dbReference type="ChEBI" id="CHEBI:17319"/>
            <dbReference type="ChEBI" id="CHEBI:33737"/>
            <dbReference type="ChEBI" id="CHEBI:33738"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:74411"/>
            <dbReference type="ChEBI" id="CHEBI:74497"/>
            <dbReference type="EC" id="2.1.1.192"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="1">Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="miscellaneous">
        <text evidence="1">Reaction proceeds by a ping-pong mechanism involving intermediate methylation of a conserved cysteine residue.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the radical SAM superfamily. RlmN family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.192" evidence="1"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08988.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516822.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q1LLI8"/>
    <dbReference type="SMR" id="Q1LLI8"/>
    <dbReference type="STRING" id="266264.Rmet_2109"/>
    <dbReference type="EnsemblBacteria" id="ABF08988">
        <property type="protein sequence ID" value="ABF08988"/>
        <property type="gene ID" value="Rmet_2109"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2109"/>
    <dbReference type="eggNOG" id="COG0820">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029101_0_0_4"/>
    <dbReference type="OMA" id="QVGCSLD"/>
    <dbReference type="OrthoDB" id="1111428at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070040">
        <property type="term" value="F:rRNA (adenine-C2-)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002935">
        <property type="term" value="F:tRNA (adenine-C2-)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070475">
        <property type="term" value="P:rRNA base methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01849">
        <property type="entry name" value="RNA_methyltr_RlmN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040072">
        <property type="entry name" value="Methyltransferase_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027492">
        <property type="entry name" value="RNA_MTrfase_RlmN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004383">
        <property type="entry name" value="rRNA_lsu_MTrfase_RlmN/Cfr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30544">
        <property type="entry name" value="PTHR30544"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006004">
        <property type="entry name" value="CHP00048"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00275">
        <property type="entry name" value="adenosine_C2_methyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00048">
        <property type="entry name" value="rRNA_mod_RlmN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0004">4Fe-4S</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-1015">Disulfide bond</keyword>
    <keyword id="KW-0408">Iron</keyword>
    <keyword id="KW-0411">Iron-sulfur</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0489">Methyltransferase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0698">rRNA processing</keyword>
    <keyword id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <keyword id="KW-0819">tRNA processing</keyword>
    <feature type="chain" id="PRO_0000350355" description="Dual-specificity RNA methyltransferase RlmN">
        <location>
            <begin position="1"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="domain" description="Radical SAM core" evidence="2">
        <location>
            <begin position="99"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="1">
        <location>
            <position position="93"/>
        </location>
    </feature>
    <feature type="active site" description="S-methylcysteine intermediate" evidence="1">
        <location>
            <position position="344"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="113"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="117"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="120"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="170"/>
            <end position="171"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="202"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="224"/>
            <end position="226"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="301"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="disulfide bond" description="(transient)" evidence="1">
        <location>
            <begin position="106"/>
            <end position="344"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01266"/>
        </source>
    </evidence>
    <sequence length="384" mass="42266" checksum="A2EAD3BE6AADAC43" modified="2006-05-30" version="1">MNNLVNLLDLDADALTAYCGELGEKPFRARQLQRWIHQFGASHFDAMTDLAKSLREKLATRAEIRSPAAISDHTSSDGTRKWLLDVGAGNAVETVYIPEDTRGTLCVSSQAGCAVNCRFCSTGKQGFSRNLSTGEIIGQLWMAEFAMRAQLGRGPKDERVISNVVMMGMGEPLLNYDAVVPAMRLMLDDNAYGLSRRRVTLSTSGVVPMMDRLSKDLPVALAVSLHASNDALRDVLVPLNRKYPLAELMAACRRYLEFAPRDFITFEYCMLDGVNDGVEHARELLKLVADVPCKFNLIPFNPFPESGLKRSNNEQIRRFAQVLMDAGIVTTIRKTRGDDIDAACGQLAGEVMDRTRLAERGKFGKITPLVPVADAGAPREARPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="108" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LLR5</accession>
    <name>Q1LLR5_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Transcription termination/antitermination protein NusA</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7 9" type="primary">nusA</name>
        <name evidence="9" type="ordered locus">Rmet_2032</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Participates in both transcription termination and antitermination.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Monomer. Binds directly to the core enzyme of the DNA-dependent RNA polymerase and to nascent RNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the NusA family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08911.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008650614.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2032"/>
    <dbReference type="EnsemblBacteria" id="ABF08911">
        <property type="protein sequence ID" value="ABF08911"/>
        <property type="gene ID" value="Rmet_2032"/>
    </dbReference>
    <dbReference type="GeneID" id="60821389"/>
    <dbReference type="KEGG" id="rme:Rmet_2032"/>
    <dbReference type="eggNOG" id="COG0195">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029242_0_0_4"/>
    <dbReference type="OMA" id="MDIDMSA"/>
    <dbReference type="OrthoDB" id="384865at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006353">
        <property type="term" value="P:DNA-templated transcription, termination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031564">
        <property type="term" value="P:transcription antitermination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1480.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00945_B">
        <property type="entry name" value="NusA_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010995">
        <property type="entry name" value="DNA_repair_Rad51/TF_NusA_a-hlx"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015946">
        <property type="entry name" value="KH_dom-like_a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025249">
        <property type="entry name" value="KH_dom_NusA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009019">
        <property type="entry name" value="KH_sf_prok-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030842">
        <property type="entry name" value="NusA_bac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036555">
        <property type="entry name" value="NusA_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022967">
        <property type="entry name" value="S1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003029">
        <property type="entry name" value="S1_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013735">
        <property type="entry name" value="TF_NusA_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010214">
        <property type="entry name" value="Tscrpt_termin_fac_NusA_C_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010213">
        <property type="entry name" value="Tscrpt_termination_fac_NusA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22648">
        <property type="entry name" value="PTHR22648"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13184">
        <property type="entry name" value="KH_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08529">
        <property type="entry name" value="NusA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00575">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00316">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47794">
        <property type="entry name" value="SSF47794"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54814">
        <property type="entry name" value="SSF54814"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69705">
        <property type="entry name" value="SSF69705"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01953">
        <property type="entry name" value="NusA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01954">
        <property type="entry name" value="nusA_Cterm_rpt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50126">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="6 7" id="KW-0804">Transcription</keyword>
    <keyword evidence="3 7" id="KW-0889">Transcription antitermination</keyword>
    <keyword evidence="5 7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="1 7" id="KW-0806">Transcription termination</keyword>
    <feature type="domain" description="S1 motif" evidence="8">
        <location>
            <begin position="138"/>
            <end position="202"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00945"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ABF08911.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="491" mass="55054" checksum="300A8E50A6C0E41B" modified="2006-05-30" version="1">MSREVLLLVDALAREKNVDKDVVFGALEAALASATKKRFEEDVDIRVHIDRESGEHETFRRWLVVPDEQGLQEPDKQILLFEAREQNADIQLDDYIEEQIESVEFGRIGAQAAKQVILQRIRDAEREQILNDYLDRGEKIMTGTVKRADKKGLIVESGRVEALLARDQIIPKENLRTGDRVRAYILNVDRAARGPQIELSRTAPEFLIKLFENEVPEMEQGLLEIKAAARDPGVRAKIAVVAHDKRIDPIGTCVGVRGTRVTAVRNEIGGEAVDIVLWSEDPAQFVIGALAPAQVQSIVVDEEKHSMDVVVDEENLAVSIGRSGQNVRLASELTGWQINIMTQEESAQKQAEESEVVRKLFMAKLDVDEEVADILIEEGFSSLEEVAYVPISEMMEIEAFDEDTVNELRNRARDALLTMELAREEKVEEVSQDLRSLDGLTPELIGKLAEGNIHTRDDLAELAVDELVELAGVGEEEAKALIMKAREHWFN</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-10-17" modified="2022-08-03" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LLY6</accession>
    <name>CRCB_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Putative fluoride ion transporter CrcB</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">crcB</name>
        <name type="ordered locus">Rmet_1961</name>
    </gene>
    <organism>
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Important for reducing fluoride concentration in the cell, thus reducing its toxicity.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the CrcB (TC 9.B.71) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08840.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008645434.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q1LLY6"/>
    <dbReference type="SMR" id="Q1LLY6"/>
    <dbReference type="STRING" id="266264.Rmet_1961"/>
    <dbReference type="EnsemblBacteria" id="ABF08840">
        <property type="protein sequence ID" value="ABF08840"/>
        <property type="gene ID" value="Rmet_1961"/>
    </dbReference>
    <dbReference type="GeneID" id="60821433"/>
    <dbReference type="KEGG" id="rme:Rmet_1961"/>
    <dbReference type="eggNOG" id="COG0239">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_114342_3_3_4"/>
    <dbReference type="OMA" id="EWRLFLI"/>
    <dbReference type="OrthoDB" id="1942863at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005887">
        <property type="term" value="C:integral component of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903425">
        <property type="term" value="F:fluoride transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00454">
        <property type="entry name" value="CrcB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003691">
        <property type="entry name" value="CrcB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28259">
        <property type="entry name" value="PTHR28259"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02537">
        <property type="entry name" value="CRCB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00494">
        <property type="entry name" value="crcB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0997">Cell inner membrane</keyword>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <keyword id="KW-0813">Transport</keyword>
    <feature type="chain" id="PRO_0000252921" description="Putative fluoride ion transporter CrcB">
        <location>
            <begin position="1"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="5"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="36"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="69"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="99"/>
            <end position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00454"/>
        </source>
    </evidence>
    <sequence length="126" mass="13468" checksum="0A916AEDD4DB95B0" modified="2006-05-30" version="1">MGPMGFVAVGVGAAVGAWLRWFFSVTWNAINPALPYGTLASNLVGGYLIGLAVAFFETHPSLPPEWRLLAVTGFLGGLTTFSTFSSEVIANLMAGDYRWAMLHLAMHLGGSLLLTAFGIWSYRALA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LMA4</accession>
    <name>Q1LMA4_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Ssterase/lipase</fullName>
            <ecNumber evidence="4">3.1.1.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Rmet_1843</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the 'GDXG' lipolytic enzyme family.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.-" evidence="4"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08722.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516573.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1843"/>
    <dbReference type="ESTHER" id="ralme-q1lma4">
        <property type="family name" value="Hormone-sensitive_lipase_like"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF08722">
        <property type="protein sequence ID" value="ABF08722"/>
        <property type="gene ID" value="Rmet_1843"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1843"/>
    <dbReference type="eggNOG" id="COG0657">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012494_6_4_4"/>
    <dbReference type="OMA" id="QWLIYPR"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013094">
        <property type="entry name" value="AB_hydrolase_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002168">
        <property type="entry name" value="Lipase_GDXG_HIS_AS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07859">
        <property type="entry name" value="Abhydrolase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="SSF53474"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01173">
        <property type="entry name" value="LIPASE_GDXG_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Abhydrolase_3" evidence="3">
        <location>
            <begin position="109"/>
            <end position="318"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF07859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF08722.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="344" mass="37116" checksum="851591914CE6B278" modified="2006-05-30" version="1">MLLYGITGMIWKSNDRSEIEHQYMPAQPLDSQVAQLLDLIARAKRPAIHTMDPVDAKIAYEKSAPILDLSPAPVHGVEDFQVTARDGHAIPVRLYSPREASWADPLPLLVYFHGGGFTVGSVNSHDSLCRMFCNGAECLVLSVDYRLGPEWKFPVAANDAFDVLHWVFEEAARIGADATRIALGGDSAGGTLAAACAVEARDHGLAPVLQMLIYPGTCARQDTPSHRALAEGYLLTAEMIQWFFSQYLDVDASRDDWRFAPLDGGGEGADVRGCCPAWIAVAGYDPLHDEGVAYAAKLEAAGVVASLTDYPSMIHDFFKLGRFIGAVAGAHADATAALRRAFGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="52" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LML7</accession>
    <name>Q1LML7_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_1730</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08609.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516462.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1730"/>
    <dbReference type="EnsemblBacteria" id="ABF08609">
        <property type="protein sequence ID" value="ABF08609"/>
        <property type="gene ID" value="Rmet_1730"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1730"/>
    <dbReference type="eggNOG" id="COG3313">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_162538_6_2_4"/>
    <dbReference type="OrthoDB" id="2068388at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010710">
        <property type="entry name" value="DUF1289"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35175">
        <property type="entry name" value="PTHR35175"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06945">
        <property type="entry name" value="DUF1289"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF08609.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="66" mass="7204" checksum="072BD29078099D7D" modified="2006-05-30" version="1">MPAANPCINICRMDLAGKYCQGCRRTTVEIGLWDTMTEVQRIEVLADLPLRALGRGASQQPTSVPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LN54</accession>
    <name>Q1LN54_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Hydrogenase maturation factor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">hypD2</name>
        <name evidence="4" type="ordered locus">Rmet_1539</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the HypD family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08422.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516279.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1539"/>
    <dbReference type="EnsemblBacteria" id="ABF08422">
        <property type="protein sequence ID" value="ABF08422"/>
        <property type="gene ID" value="Rmet_1539"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1539"/>
    <dbReference type="eggNOG" id="COG0409">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_048562_0_0_4"/>
    <dbReference type="OMA" id="MRYLDEY"/>
    <dbReference type="OrthoDB" id="666873at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11740">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.20.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002780">
        <property type="entry name" value="Hyd_form_HypD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042243">
        <property type="entry name" value="HypD_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042244">
        <property type="entry name" value="HypD_2_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30149">
        <property type="entry name" value="PTHR30149"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01924">
        <property type="entry name" value="HypD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005622">
        <property type="entry name" value="Hydrgn_mat_hypD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00075">
        <property type="entry name" value="hypD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRNR" id="PIRNR005622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF08422.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="380" mass="41396" checksum="55FC30811C251DB5" modified="2006-05-30" version="1">MKYVDEFRDGELARTLAAKIARDADSGRSYSFMEFCGGHTHAISRYGVSDLLPANVRMIHGPGCPVCVLPIGRIDLAIKLALERGVILCSYGDTLRIPASESLSLLKAKARGADIRMVYSAADALAIAQQNPQREVVFFAIGFETTTPPTALVIKQAEAARVTNFSVLCNHVLTPSAIITILESPEVRKLGTVPLDGFVGPAHVSTVIGSRPYEFFAEEYRKPVVIAGFEPLDVMQAILMLVTQVNEGRAEVENEFTRAVTRDGNQKAQALVSEVFELRCSFEWRGLGEVPYSALRIRASYRLFDAEARFGLAYRPVPDNKACECGAILRGVKKPTDCKLFATACTPENPMGSCMVSSEGACAAHYSYGRFKDIEVVVAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="67" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LNP8</accession>
    <name>Q1LNP8_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Conjugal transfer TrbJ signal peptide protein (Protein belongs to CMGI-2)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">trbJ</name>
        <name evidence="3" type="ordered locus">Rmet_1345</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08228.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516109.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1345"/>
    <dbReference type="EnsemblBacteria" id="ABF08228">
        <property type="protein sequence ID" value="ABF08228"/>
        <property type="gene ID" value="Rmet_1345"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1345"/>
    <dbReference type="eggNOG" id="COG5314">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_065326_1_0_4"/>
    <dbReference type="OMA" id="QKMEPRW"/>
    <dbReference type="OrthoDB" id="1557327at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014147">
        <property type="entry name" value="T4SS_TrbJ"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02780">
        <property type="entry name" value="TrbJ_Ti"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193246" evidence="2">
        <location>
            <begin position="27"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="39"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF08228.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="245" mass="27224" checksum="2FF43CDB0F58BEA5" modified="2006-05-30" version="1">MKIRALSISLAAALSVSLLAVQPASAITVFDPSNFVQNTLTAIRTLEQINNQIQQLQNEAQMLMNQARNLASLPSSVVNQLRANLATTERLIAQARGLAYDVTNLDREFARLYPEQYAATVSGDQMYRDAQERWRNTLNGLQTTMQMQAQVSQNLGEDESVLADLVSKSQSAHGALQAMQAMNQLLALQAKQSIQAQRLQITQDRAASLELARQAAATERAREVRRRFLGEGTPYTPQTVNFYRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-10-07" version="66" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LNW5</accession>
    <name>Q1LNW5_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_1276</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08161.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1276"/>
    <dbReference type="EnsemblBacteria" id="ABF08161">
        <property type="protein sequence ID" value="ABF08161"/>
        <property type="gene ID" value="Rmet_1276"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1276"/>
    <dbReference type="eggNOG" id="ENOG5033X2F">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_172554_0_0_4"/>
    <dbReference type="OMA" id="MTPRAGC"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF08161.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="111" mass="12136" checksum="91610FE428DFB609" modified="2006-05-30" version="1">MKTESRMSEAVAQWAGHLEAIEAEGISAQAYARREGVSAASLYYWRRRLKSRQPSAAIEAAPRLVSVHLGETPEPVRCTLLVAPGVRLELMQLPSAAWLAQLAAAVARQVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="60" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LP61</accession>
    <name>Q1LP61_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_1179</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08065.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515959.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1179"/>
    <dbReference type="EnsemblBacteria" id="ABF08065">
        <property type="protein sequence ID" value="ABF08065"/>
        <property type="gene ID" value="Rmet_1179"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1179"/>
    <dbReference type="eggNOG" id="COG1326">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_194590_0_0_4"/>
    <dbReference type="OMA" id="GTQWETS"/>
    <dbReference type="OrthoDB" id="2046780at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="44"/>
            <end position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF08065.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="79" mass="8789" checksum="2ACA876E39BD25AD" modified="2006-05-30" version="1">MFVCHNQSCGARWAPDEVDIHNEGQGPLFRCPLCGARNHLKANQGRDGAVTYKQIPREHVAKTPPSAEAKRATPPRRRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="70" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LPD9</accession>
    <name>Q1LPD9_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">6-carboxy-5,6,7,8-tetrahydropterin synthase</fullName>
            <ecNumber evidence="5">4.1.2.50</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Queuosine biosynthesis protein QueD</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">Rmet_1101</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>7,8-dihydroneopterin 3'-triphosphate + H2O = 6-carboxy-5,6,7,8-tetrahydropterin + acetaldehyde + 2 H(+) + triphosphate</text>
            <dbReference type="Rhea" id="RHEA:27966"/>
            <dbReference type="ChEBI" id="CHEBI:15343"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:18036"/>
            <dbReference type="ChEBI" id="CHEBI:58462"/>
            <dbReference type="ChEBI" id="CHEBI:61032"/>
            <dbReference type="EC" id="4.1.2.50"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Purine metabolism; 7-cyano-7-deazaguanine biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the PTPS family. QueD subfamily.</text>
    </comment>
    <dbReference type="EC" id="4.1.2.50" evidence="5"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07987.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1101"/>
    <dbReference type="EnsemblBacteria" id="ABF07987">
        <property type="protein sequence ID" value="ABF07987"/>
        <property type="gene ID" value="Rmet_1101"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1101"/>
    <dbReference type="eggNOG" id="COG0720">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_111016_0_0_4"/>
    <dbReference type="OMA" id="DHAHHFW"/>
    <dbReference type="UniPathway" id="UPA00391"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070497">
        <property type="term" value="F:6-carboxy-5,6,7,8-tetrahydropterin synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.479.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007115">
        <property type="entry name" value="6-PTP_synth/QueD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038418">
        <property type="entry name" value="6-PTP_synth/QueD_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12589">
        <property type="entry name" value="PTHR12589"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01242">
        <property type="entry name" value="PTPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 11" id="KW-0456">Lyase</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001293"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00018141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00031449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ABF07987.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="193" mass="21801" checksum="48AF0A7217ACC411" modified="2006-05-30" version="1">MSCDEAMLIRKLFRFENAHIVRGCSTRRCSHSIHGHSYKIELLLEAHSLDHGQMVYDFGLLKGGAFTLIDAFDHAVTLWTGDDPAYLAAMRQHSERWVLIPVSPSAEQFSRLFFLLVDAVLATTALRNGEGDVRLHSVIVHETETGYAQCFREDAENPRMGRIDLESIVFAPAITAQWSDPALFERLKRARAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="106" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LPP0</accession>
    <name>Q1LPP0_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Heat-inducible transcription repressor HrcA</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">hrcA</name>
        <name evidence="7" type="ordered locus">Rmet_1000</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Negative regulator of class I heat shock genes (grpE-dnaK-dnaJ and groELS operons). Prevents heat-shock induction of these operons.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the HrcA family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07886.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008649330.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1000"/>
    <dbReference type="EnsemblBacteria" id="ABF07886">
        <property type="protein sequence ID" value="ABF07886"/>
        <property type="gene ID" value="Rmet_1000"/>
    </dbReference>
    <dbReference type="GeneID" id="60821861"/>
    <dbReference type="KEGG" id="rme:Rmet_1000"/>
    <dbReference type="eggNOG" id="COG1420">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_050019_0_0_4"/>
    <dbReference type="OMA" id="DYPGTMG"/>
    <dbReference type="OrthoDB" id="1510750at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045892">
        <property type="term" value="P:negative regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00081">
        <property type="entry name" value="HrcA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029016">
        <property type="entry name" value="GAF-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002571">
        <property type="entry name" value="HrcA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021153">
        <property type="entry name" value="HrcA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005104">
        <property type="entry name" value="WHTH_HrcA_DNA-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34824">
        <property type="entry name" value="PTHR34824"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01628">
        <property type="entry name" value="HrcA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03444">
        <property type="entry name" value="HrcA_DNA-bdg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005485">
        <property type="entry name" value="HrcA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00331">
        <property type="entry name" value="hrcA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0678">Repressor</keyword>
    <keyword evidence="2 4" id="KW-0346">Stress response</keyword>
    <keyword evidence="3 4" id="KW-0804">Transcription</keyword>
    <keyword evidence="1 4" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HrcA_DNA-bdg" evidence="6">
        <location>
            <begin position="5"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="domain" description="HrcA" evidence="5">
        <location>
            <begin position="113"/>
            <end position="331"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01628"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF03444"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABF07886.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="348" mass="38365" checksum="D939A575F8F2F624" modified="2006-05-30" version="1">MDERSKKLLKTLIERYIVEGQPVGSRTLSKFSGLDLSPATIRNVMSDLEEMGFIASPHTSAGRIPTPRGYRLFVDTMLTARPLERAPEFSAELAELTGQIQGQLGGQQFGPQRMITAAAHTLSNLSHFAGVVMTPRRAQSFRQVEFMRLSDKRILLIIVSPEGDVQNRIIQTDLAYTPGQLIEAANFFNSHYAGMSFDAVRDHLRVELQDLRRDMSALMQAAVEAGSSAMAEDNEQVFISGERKLLEVEDLSSSMDKLRRLFDVFEHKTSLLQLLDVSSHAQGVQIFIGGESQLVPIEDMAVITAPYGVDGQIVGTLGVIGPTRMAYERVIPIVDITARLLSSALSQS</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-03-03" modified="2022-08-03" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LQ15</accession>
    <name>F16A1_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Fructose-1,6-bisphosphatase class 1 1</fullName>
            <shortName evidence="1">FBPase class 1 1</shortName>
            <ecNumber evidence="1">3.1.3.11</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">D-fructose-1,6-bisphosphate 1-phosphohydrolase class 1 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">fbp1</name>
        <name type="ordered locus">Rmet_0875</name>
    </gene>
    <organism>
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>beta-D-fructose 1,6-bisphosphate + H2O = beta-D-fructose 6-phosphate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:11064"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:32966"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57634"/>
            <dbReference type="EC" id="3.1.3.11"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="1">Binds 2 magnesium ions per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Carbohydrate biosynthesis; gluconeogenesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homotetramer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the FBPase class 1 family.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.11" evidence="1"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07761.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008649749.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q1LQ15"/>
    <dbReference type="SMR" id="Q1LQ15"/>
    <dbReference type="STRING" id="266264.Rmet_0875"/>
    <dbReference type="EnsemblBacteria" id="ABF07761">
        <property type="protein sequence ID" value="ABF07761"/>
        <property type="gene ID" value="Rmet_0875"/>
    </dbReference>
    <dbReference type="GeneID" id="60825363"/>
    <dbReference type="KEGG" id="rme:Rmet_0875"/>
    <dbReference type="eggNOG" id="COG0158">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039977_0_0_4"/>
    <dbReference type="OMA" id="YIPENCP"/>
    <dbReference type="OrthoDB" id="945770at2"/>
    <dbReference type="UniPathway" id="UPA00138"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042132">
        <property type="term" value="F:fructose 1,6-bisphosphate 1-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006094">
        <property type="term" value="P:gluconeogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00354">
        <property type="entry name" value="FBPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01855">
        <property type="entry name" value="FBPase_class1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044015">
        <property type="entry name" value="FBPase_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000146">
        <property type="entry name" value="FBPase_class-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033391">
        <property type="entry name" value="FBPase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028343">
        <property type="entry name" value="FBPtase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11556">
        <property type="entry name" value="PTHR11556"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00316">
        <property type="entry name" value="FBPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18913">
        <property type="entry name" value="FBPase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF500210">
        <property type="entry name" value="FBPtase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000904">
        <property type="entry name" value="FBPtase_SBPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00115">
        <property type="entry name" value="F16BPHPHTASE"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000364662" description="Fructose-1,6-bisphosphatase class 1 1">
        <location>
            <begin position="1"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="91"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="113"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="113"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="115"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="116"/>
            <end position="119"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="116"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="208"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="274"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="280"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01855"/>
        </source>
    </evidence>
    <sequence length="338" mass="37526" checksum="1F8360B1B23DA30D" modified="2006-05-30" version="1">MTRISLTRYLVEEQRKHNTIEPELRLLIEVVARACKAISNAVSKGALAGVLGSAGTGNVQGETQQKLDVIANEVLLDANEWGGHLAAMASEEMDSFYEIPNRYPKGEYLLLFDPLDGSSNIDVNVSIGTIFSVLHMHKPGQTVTEHDFMQPGTQQVAAGYAVYGPQTTLVLTVGNGVHMFTLDREAGSFVLTQSDVMIPEDTKEFAINMSNMRHWAPPVRRYIDECLAGDEGPRGKNFNMRWIASMVADVHRILTRGGVFMYPWDKREPEKPGKLRLMYEANPMAFLVEQAGGAATNGQQRILDVEPTKLHQRVSVILGSKNEVERVTRYHQEDAAKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="103" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LQ39</accession>
    <name>Q1LQ39_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">dITP/XTP pyrophosphatase</fullName>
            <ecNumber evidence="7">3.6.1.66</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Non-canonical purine NTP pyrophosphatase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="7">Non-standard purine NTP pyrophosphatase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="7">Nucleoside-triphosphate diphosphatase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="7">Nucleoside-triphosphate pyrophosphatase</fullName>
            <shortName evidence="7">NTPase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">yggV</name>
        <name evidence="9" type="ordered locus">Rmet_0851</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Pyrophosphatase that catalyzes the hydrolysis of nucleoside triphosphates to their monophosphate derivatives, with a high preference for the non-canonical purine nucleotides XTP (xanthosine triphosphate), dITP (deoxyinosine triphosphate) and ITP. Seems to function as a house-cleaning enzyme that removes non-canonical purine nucleotides from the nucleotide pool, thus preventing their incorporation into DNA/RNA and avoiding chromosomal lesions.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>H2O + ITP = diphosphate + H(+) + IMP</text>
            <dbReference type="Rhea" id="RHEA:29399"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
            <dbReference type="ChEBI" id="CHEBI:61402"/>
            <dbReference type="EC" id="3.6.1.66"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>H2O + XTP = diphosphate + H(+) + XMP</text>
            <dbReference type="Rhea" id="RHEA:28610"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57464"/>
            <dbReference type="ChEBI" id="CHEBI:61314"/>
            <dbReference type="EC" id="3.6.1.66"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>dITP + H2O = dIMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:28342"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61194"/>
            <dbReference type="ChEBI" id="CHEBI:61382"/>
            <dbReference type="EC" id="3.6.1.66"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="7">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="7">Binds 1 Mg(2+) ion per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 7 8">Belongs to the HAM1 NTPase family.</text>
    </comment>
    <dbReference type="EC" id="3.6.1.66" evidence="7"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07737.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515679.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0851"/>
    <dbReference type="EnsemblBacteria" id="ABF07737">
        <property type="protein sequence ID" value="ABF07737"/>
        <property type="gene ID" value="Rmet_0851"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0851"/>
    <dbReference type="eggNOG" id="COG0127">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_082080_0_3_4"/>
    <dbReference type="OMA" id="DCFADDT"/>
    <dbReference type="OrthoDB" id="1824064at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035870">
        <property type="term" value="F:dITP diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036220">
        <property type="term" value="F:ITP diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017111">
        <property type="term" value="F:nucleoside-triphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036222">
        <property type="term" value="F:XTP diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009117">
        <property type="term" value="P:nucleotide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009146">
        <property type="term" value="P:purine nucleoside triphosphate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00515">
        <property type="entry name" value="HAM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.950.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01405">
        <property type="entry name" value="Non_canon_purine_NTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020922">
        <property type="entry name" value="dITP/XTP_pyrophosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002637">
        <property type="entry name" value="Ham1p-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029001">
        <property type="entry name" value="ITPase-like_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11067">
        <property type="entry name" value="PTHR11067"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01725">
        <property type="entry name" value="Ham1p_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52972">
        <property type="entry name" value="SSF52972"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00042">
        <property type="entry name" value="TIGR00042"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 7" id="KW-0460">Magnesium</keyword>
    <keyword evidence="2 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 7" id="KW-0546">Nucleotide metabolism</keyword>
    <keyword evidence="3 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Substrate binding" evidence="7">
        <location>
            <begin position="8"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="7">
        <location>
            <begin position="155"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="7">
        <location>
            <begin position="183"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="7">
        <location>
            <position position="69"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="40"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="69"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="70"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="178"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ABF07737.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="204" mass="21622" checksum="F94C2EDE78889E31" modified="2006-05-30" version="1">MQRLVLASNNPGKLREFSALLSPLGFDVVPQGELGVPEAEEPFATFVENALTKARHAARLTGMPALADDSGICVEALDGAPGVYSARYAQTVGKPKSDAANNAHLISQLAGKLNRRAHYYCVLVMVRHADDPRPIIAEGIWAGEVVDAPRGNGGFGYDPHFLLPSVGKTAAELSAEEKNAISHRALALQELVARLQTAGIKSCA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LQ81</accession>
    <name>Q1LQ81_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">L-threonine aldolase</fullName>
            <ecNumber evidence="3">4.1.2.48</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">ltaA</name>
        <name evidence="5" type="ordered locus">Rmet_0809</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Catalyzes the cleavage of L-allo-threonine and L-threonine to glycine and acetaldehyde.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>L-allo-threonine = acetaldehyde + glycine</text>
            <dbReference type="Rhea" id="RHEA:26209"/>
            <dbReference type="ChEBI" id="CHEBI:15343"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
            <dbReference type="ChEBI" id="CHEBI:58585"/>
            <dbReference type="EC" id="4.1.2.48"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>L-threonine = acetaldehyde + glycine</text>
            <dbReference type="Rhea" id="RHEA:19625"/>
            <dbReference type="ChEBI" id="CHEBI:15343"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
            <dbReference type="ChEBI" id="CHEBI:57926"/>
            <dbReference type="EC" id="4.1.2.48"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 3">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the threonine aldolase family.</text>
    </comment>
    <dbReference type="EC" id="4.1.2.48" evidence="3"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07695.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0809"/>
    <dbReference type="EnsemblBacteria" id="ABF07695">
        <property type="protein sequence ID" value="ABF07695"/>
        <property type="gene ID" value="Rmet_0809"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0809"/>
    <dbReference type="eggNOG" id="COG2008">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_049619_0_0_4"/>
    <dbReference type="OMA" id="ETDCEVF"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008732">
        <property type="term" value="F:L-allo-threonine aldolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006567">
        <property type="term" value="P:threonine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001597">
        <property type="entry name" value="ArAA_b-elim_lyase/Thr_aldolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026273">
        <property type="entry name" value="Low_specificity_L-TA_bact"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01212">
        <property type="entry name" value="Beta_elim_lyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038940">
        <property type="entry name" value="Low_specificity_LTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="SSF53383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0456">Lyase</keyword>
    <keyword evidence="2 3" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Beta_elim_lyase" evidence="4">
        <location>
            <begin position="10"/>
            <end position="299"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRNR" id="PIRNR038940"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABF07695.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="350" mass="38312" checksum="DD80B5EC0C782E97" modified="2006-05-30" version="1">MTSAVSTMQQFASDNYAGFCPESLKYFLEANASGHEKAYGDDSWTQKVCDRIRDLFQTDCEVFFVFNGTAANSLALSSLCQSYHSVICHELAHIETDECGGPEFFSNGSKLLTAPGENGKLTPDAVEALVTRRADIHYPKPRVVSLTQATEVGTVYTVEEVRAIAAIAKRRQLRVHMDGARFANAVASLGVHPSEITWRAGVDVLCFGGTKNGLPVGEAVVFFDRKLAEDFAYRVKQAGQLASKMRFISAPWLGMLENDVWLNNARHANAMATLLHQRIADIPGVRIMFPTEANAVFAELPLPAIEALRARGWLFYTFIGAGGCRLMCAWDLQPDTVEAFAADIRTACSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="69" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LQ83</accession>
    <name>Q1LQ83_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Aldolase class 2 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_0807</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07693.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515642.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0807"/>
    <dbReference type="EnsemblBacteria" id="ABF07693">
        <property type="protein sequence ID" value="ABF07693"/>
        <property type="gene ID" value="Rmet_0807"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0807"/>
    <dbReference type="eggNOG" id="COG0235">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006033_0_0_4"/>
    <dbReference type="OMA" id="YWEFQPD"/>
    <dbReference type="OrthoDB" id="599627at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.225.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001303">
        <property type="entry name" value="Aldolase_II/adducin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036409">
        <property type="entry name" value="Aldolase_II/adducin_N_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00596">
        <property type="entry name" value="Aldolase_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01007">
        <property type="entry name" value="Aldolase_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53639">
        <property type="entry name" value="SSF53639"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aldolase_II" evidence="1">
        <location>
            <begin position="26"/>
            <end position="206"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM01007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF07693.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="257" mass="27433" checksum="15FECE1841C1D24A" modified="2006-05-30" version="1">MSFALQPGRAAGPAFPISDEEARVRVDLAAAYRLAALERWDDLIYTHISATVPGEPDHFLINPFGFAFEEIRASDLVKINSRGEVVGETVHPVNVTGFALHGAVHAARPDAHCVMHLHNTAGIAVSAQAEGLLPLSQHAMRFHGRIAYHDYEGLAFSPAEGGRLTGSLGGHPAMLLRNHGTLTVGRTVAEAYVLMATLIKACEIQLGAQLGSATVQPSAAIADKTSLQLYDGGAVEGAMEWPALLRKLDKIDPSYKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LQF1</accession>
    <name>Q1LQF1_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Acyl-CoA dehydrogenase</fullName>
            <ecNumber evidence="10">1.3.8.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">Rmet_0739</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the acyl-CoA dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.3.8.-" evidence="10"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07625.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515582.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0739"/>
    <dbReference type="EnsemblBacteria" id="ABF07625">
        <property type="protein sequence ID" value="ABF07625"/>
        <property type="gene ID" value="Rmet_0739"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0739"/>
    <dbReference type="eggNOG" id="COG1960">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018204_12_2_4"/>
    <dbReference type="OMA" id="SIMKWCG"/>
    <dbReference type="OrthoDB" id="433393at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016627">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-CH group of donors"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.540.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.110.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025878">
        <property type="entry name" value="Acyl-CoA_dh-like_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020953">
        <property type="entry name" value="Acyl-CoA_DH_N_bac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006091">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046373">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036250">
        <property type="entry name" value="AcylCo_DH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009075">
        <property type="entry name" value="AcylCo_DH/oxidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013786">
        <property type="entry name" value="AcylCoA_DH/ox_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037069">
        <property type="entry name" value="AcylCoA_DH/ox_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009100">
        <property type="entry name" value="AcylCoA_DH/oxidase_NM_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00441">
        <property type="entry name" value="Acyl-CoA_dh_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12806">
        <property type="entry name" value="Acyl-CoA_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02770">
        <property type="entry name" value="Acyl-CoA_dh_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02771">
        <property type="entry name" value="Acyl-CoA_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12418">
        <property type="entry name" value="AcylCoA_DH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47203">
        <property type="entry name" value="SSF47203"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56645">
        <property type="entry name" value="SSF56645"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0274">FAD</keyword>
    <keyword evidence="3" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="10" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AcylCoA_DH_N" evidence="8">
        <location>
            <begin position="2"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA_dh_N" evidence="7">
        <location>
            <begin position="35"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA_dh_M" evidence="6">
        <location>
            <begin position="161"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA_dh_1" evidence="5">
        <location>
            <begin position="283"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA_dh_C" evidence="9">
        <location>
            <begin position="467"/>
            <end position="587"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF02770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF02771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF12418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF12806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ABF07625.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="594" mass="63292" checksum="A879771DC7A15B88" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LQH4</accession>
    <name>Q1LQH4_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Bifunctional chorismate mutase/prephenate dehydratase</fullName>
            <ecNumber evidence="8">4.2.1.51</ecNumber>
            <ecNumber evidence="7">5.4.99.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="18">Chorismate mutase-prephenate dehydratase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="17">p-protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="24" type="primary">pheA</name>
        <name evidence="24" type="ordered locus">Rmet_0716</name>
    </gene>
    <organism evidence="24 25">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="25" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="25">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Catalyzes the Claisen rearrangement of chorismate to prephenate and the decarboxylation/dehydration of prephenate to phenylpyruvate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>H(+) + prephenate = 3-phenylpyruvate + CO2 + H2O</text>
            <dbReference type="Rhea" id="RHEA:21648"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:18005"/>
            <dbReference type="ChEBI" id="CHEBI:29934"/>
            <dbReference type="EC" id="4.2.1.51"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>chorismate = prephenate</text>
            <dbReference type="Rhea" id="RHEA:13897"/>
            <dbReference type="ChEBI" id="CHEBI:29748"/>
            <dbReference type="ChEBI" id="CHEBI:29934"/>
            <dbReference type="EC" id="5.4.99.5"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="5">Amino-acid biosynthesis; L-phenylalanine biosynthesis; phenylpyruvate from prephenate: step 1/1.</text>
    </comment>
    <comment type="pathway">
        <text evidence="6">Metabolic intermediate biosynthesis; prephenate biosynthesis; prephenate from chorismate: step 1/1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="4.2.1.51" evidence="8"/>
    <dbReference type="EC" id="5.4.99.5" evidence="7"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07602.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515565.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0716"/>
    <dbReference type="EnsemblBacteria" id="ABF07602">
        <property type="protein sequence ID" value="ABF07602"/>
        <property type="gene ID" value="Rmet_0716"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0716"/>
    <dbReference type="eggNOG" id="COG0077">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG1605">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035008_0_1_4"/>
    <dbReference type="OMA" id="PLMIYRE"/>
    <dbReference type="OrthoDB" id="1280729at2"/>
    <dbReference type="UniPathway" id="UPA00120">
        <property type="reaction ID" value="UER00203"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00121">
        <property type="reaction ID" value="UER00345"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004106">
        <property type="term" value="F:chorismate mutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004664">
        <property type="term" value="F:prephenate dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046417">
        <property type="term" value="P:chorismate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009094">
        <property type="term" value="P:L-phenylalanine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.59.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045865">
        <property type="entry name" value="ACT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002912">
        <property type="entry name" value="ACT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008242">
        <property type="entry name" value="Chor_mutase/pphenate_deHydtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036263">
        <property type="entry name" value="Chorismate_II_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036979">
        <property type="entry name" value="CM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002701">
        <property type="entry name" value="CM_II_prokaryot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010957">
        <property type="entry name" value="G/b/e-P-prot_chorismate_mutase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001086">
        <property type="entry name" value="Preph_deHydtase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01842">
        <property type="entry name" value="ACT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01817">
        <property type="entry name" value="CM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00800">
        <property type="entry name" value="PDT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001500">
        <property type="entry name" value="Chor_mut_pdt_Ppr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00830">
        <property type="entry name" value="CM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48600">
        <property type="entry name" value="SSF48600"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55021">
        <property type="entry name" value="SSF55021"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01807">
        <property type="entry name" value="CM_P2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51671">
        <property type="entry name" value="ACT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51168">
        <property type="entry name" value="CHORISMATE_MUT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51171">
        <property type="entry name" value="PREPHENATE_DEHYDR_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="11" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="12" id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword evidence="10" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="14 24" id="KW-0413">Isomerase</keyword>
    <keyword evidence="15" id="KW-0456">Lyase</keyword>
    <keyword evidence="16" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="13" id="KW-0584">Phenylalanine biosynthesis</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Chorismate mutase" evidence="21">
        <location>
            <begin position="30"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="domain" description="Prephenate dehydratase" evidence="22">
        <location>
            <begin position="120"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="domain" description="ACT" evidence="23">
        <location>
            <begin position="307"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="site" description="Essential for prephenate dehydratase activity" evidence="19">
        <location>
            <position position="288"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00000913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00002364"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00004817"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00012404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00013147"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00014401"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00031175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00031520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PIRSR" id="PIRSR001500-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS51168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS51171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS51671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="EMBL" id="ABF07602.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="387" mass="42141" checksum="2B7E318F90FC726C" modified="2006-05-30" version="1">MTEQDKSPAADAPAQEAGRQDAPGPSAQEQALSADLAPLRTQIDTVDRELLALLNRRAQLALDVGEVKKKYGAPVFRPERELQVIRKVQGANPGPLLDESVASIWREVMSACRGLEKPLEIAFLGPAGTFSEQALYAHFGHEVTGVPCPSIDEVFRAVEAGTVEYGVVPVENSTEGAVSRTLDLFLQTSLKISGEIALKVHHNLMASTPDMKGVTVVRAHAQALAQCQNWLSANYPHLERQAVSSNAEAARMASEDPTVAAIAGETAANRYHLHIIRSHIQDDPHNRTRFAVIGRYETEPSGSDQTSLILSVPNKAGAVYQLLAPLAENGVSMCRFESRPARSGAWEYYFYVDVEGHQHEPAVTRAIEMLRRNAAYLKVLGSYPSSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="53" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LQI3</accession>
    <name>Q1LQI3_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_0707</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07593.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008643644.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0707"/>
    <dbReference type="EnsemblBacteria" id="ABF07593">
        <property type="protein sequence ID" value="ABF07593"/>
        <property type="gene ID" value="Rmet_0707"/>
    </dbReference>
    <dbReference type="GeneID" id="60825187"/>
    <dbReference type="KEGG" id="rme:Rmet_0707"/>
    <dbReference type="eggNOG" id="ENOG5032S4A">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_111103_1_0_4"/>
    <dbReference type="OMA" id="WEWFDVE"/>
    <dbReference type="OrthoDB" id="1796959at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF07593.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="133" mass="15868" checksum="4AD0B0099D061F01" modified="2006-05-30" version="1">MSDHPRFTVNRSVCLLVPEQPFLDWVKAVDPEPVPTLTLEAIRDDASVFLLPEVADTPENALRWVEKRWRTFFEFMLGEWFDDSLWPQQISLEMFRQWFTPRFHSMVWDMAPDSPIELEDWDDEDDQVSTLLH</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="109" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LR24</accession>
    <name>Q1LR24_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Histidine kinase</fullName>
            <ecNumber evidence="2">2.7.13.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">envZ</name>
        <name evidence="9" type="ordered locus">Rmet_0516</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.</text>
            <dbReference type="EC" id="2.7.13.3"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.7.13.3" evidence="2"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07402.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515381.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0516"/>
    <dbReference type="EnsemblBacteria" id="ABF07402">
        <property type="protein sequence ID" value="ABF07402"/>
        <property type="gene ID" value="Rmet_0516"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0516"/>
    <dbReference type="eggNOG" id="COG0642">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_89_27_4"/>
    <dbReference type="OMA" id="IAAWQDV"/>
    <dbReference type="OrthoDB" id="1031685at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00082">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003660">
        <property type="entry name" value="HAMP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003661">
        <property type="entry name" value="HisK_dim/P"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036097">
        <property type="entry name" value="HisK_dim/P_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004358">
        <property type="entry name" value="Sig_transdc_His_kin-like_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00672">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00512">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00344">
        <property type="entry name" value="BCTRLSENSOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00304">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00388">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47384">
        <property type="entry name" value="SSF47384"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="SSF55874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50885">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 9" id="KW-0418">Kinase</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="145"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="domain" description="HAMP" evidence="8">
        <location>
            <begin position="165"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="7">
        <location>
            <begin position="225"/>
            <end position="422"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ABF07402.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="427" mass="47540" checksum="A04E660E79FBF457" modified="2006-05-30" version="1">MKLRIDTLFGRIALLIAAVLVISHFSWLAILRMDRRQQQIDYSVEQMIFQLDSIQRALDATPPLPLPSLVEVADASTADEHASVPQAGRARRLVEQFTRRLPPGTEVRLEDELSTPRLWIKLPKRNRWIAMPILFVHNPPPDNRLLPGVVLVVGIAIIFALLIAWQIQRPVRDMAEAAEKLSRQQLVSPLRERGPHELRQLIERFNRMVADLVRIDQERNTMLAGIAHDLKTPLARLRLRAEMLSDPKAAAGVTRDVESMSAIVEQFLTFAQSSEPTARPVPVDKRIGELAASLQEQDRKVVLELNAGDGFRMIATQLDRIIGNLVDNAYAYGEPPVCVATSRTQEGWKLIVEDEGPGIPDDAIERVTMPFVRLDPARGGNAHSGLGLAIVDRLVRQAGGRLSIANREEGGLRVEMVFPFAAMAQAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="113" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LR34</accession>
    <name>Q1LR34_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 5">N5-carboxyaminoimidazole ribonucleotide synthase</fullName>
            <shortName evidence="3 5">N5-CAIR synthase</shortName>
            <ecNumber evidence="3 5">6.3.4.18</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3 5">5-(carboxyamino)imidazole ribonucleotide synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="3 5 8" type="primary">purK</name>
        <name evidence="8" type="ordered locus">Rmet_0506</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Catalyzes the ATP-dependent conversion of 5-aminoimidazole ribonucleotide (AIR) and HCO(3)(-) to N5-carboxyaminoimidazole ribonucleotide (N5-CAIR).</text>
    </comment>
    <comment type="function">
        <text evidence="5">Catalyzes the ATP-dependent conversion of 5-aminoimidazole ribonucleotide (AIR) and HCO(3)- to N5-carboxyaminoimidazole ribonucleotide (N5-CAIR).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3 5">
            <text>5-amino-1-(5-phospho-beta-D-ribosyl)imidazole + ATP + hydrogencarbonate = 5-carboxyamino-1-(5-phospho-D-ribosyl)imidazole + ADP + 2 H(+) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:19317"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58730"/>
            <dbReference type="ChEBI" id="CHEBI:137981"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.4.18"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3 5">Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate from 5-amino-1-(5-phospho-D-ribosyl)imidazole (N5-CAIR route): step 1/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3 5">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 5">Belongs to the PurK/PurT family.</text>
    </comment>
    <dbReference type="EC" id="6.3.4.18" evidence="3 5"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07392.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515371.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0506"/>
    <dbReference type="EnsemblBacteria" id="ABF07392">
        <property type="protein sequence ID" value="ABF07392"/>
        <property type="gene ID" value="Rmet_0506"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0506"/>
    <dbReference type="eggNOG" id="COG0026">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011534_0_1_4"/>
    <dbReference type="OMA" id="ITFDHEH"/>
    <dbReference type="OrthoDB" id="1165275at2"/>
    <dbReference type="UniPathway" id="UPA00074">
        <property type="reaction ID" value="UER00942"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034028">
        <property type="term" value="F:5-(carboxyamino)imidazole ribonucleotide synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004638">
        <property type="term" value="F:phosphoribosylaminoimidazole carboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01928">
        <property type="entry name" value="PurK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011761">
        <property type="entry name" value="ATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003135">
        <property type="entry name" value="ATP-grasp_carboxylate-amine"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013815">
        <property type="entry name" value="ATP_grasp_subdomain_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016185">
        <property type="entry name" value="PreATP-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005875">
        <property type="entry name" value="PurK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040686">
        <property type="entry name" value="PurK_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011054">
        <property type="entry name" value="Rudment_hybrid_motif"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02222">
        <property type="entry name" value="ATP-grasp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17769">
        <property type="entry name" value="PurK_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51246">
        <property type="entry name" value="SSF51246"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52440">
        <property type="entry name" value="SSF52440"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01161">
        <property type="entry name" value="purK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50975">
        <property type="entry name" value="ATP_GRASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3 4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 5" id="KW-0436">Ligase</keyword>
    <keyword evidence="8" id="KW-0456">Lyase</keyword>
    <keyword evidence="3 4 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="1 3 5" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ATP-grasp" evidence="7">
        <location>
            <begin position="141"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="133"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="176"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="181"/>
            <end position="187"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="211"/>
            <end position="214"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="219"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="242"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="297"/>
            <end position="298"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU361200"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ABF07392.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="414" mass="44724" checksum="1D5B0FCAB56345A0" modified="2006-05-30" version="1">MPTDIHPHLSEAHTPESRAEFGVNSPNSPILPGAWLGMLGGGQLGRMFTHAAQSMGYRVCVLDPDQDSPAGSVADRHICAAYTDDAALAELSSLCEAVSTEFENVPSISLERLEEAGVFVAPRGYCVSIAQNRIGEKKFFASCADRTGVPPAPHWVIQHDADVDRLPDEVLPGILKTARMGYDGKGQARVKSRDDVRAAWKAMQHVPCVLERMLPLAYEVSVLAARAADGSTATWPLAENVHRDGILFSTTMPSTSVSDDISQRARNAAATIASEMGYVGVLCIEFFVLTDGTLVANEMAPRPHNSGHITMDACETSQFEQQVRAMARLPLGSTRQHSAGKMLNLLGDCWFEFGLERTPAWDEVLAQPGAKLHLYGKNDARPSRKMGHVNCVGDHAEAADRTFDAAAKALHIPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="99" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LRB1</accession>
    <name>Q1LRB1_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">tRNA-dihydrouridine synthase B</fullName>
            <ecNumber evidence="10">1.3.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10 14" type="primary">dusB</name>
        <name evidence="14" type="ordered locus">Rmet_0429</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4 10 11">Catalyzes the synthesis of 5,6-dihydrouridine (D), a modified base found in the D-loop of most tRNAs, via the reduction of the C5-C6 double bond in target uridines.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2 10">
            <text>a 5,6-dihydrouridine in tRNA + NAD(+) = a uridine in tRNA + H(+) + NADH</text>
            <dbReference type="Rhea" id="RHEA:54452"/>
            <dbReference type="Rhea" id="RHEA-COMP:13339"/>
            <dbReference type="Rhea" id="RHEA-COMP:13887"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="ChEBI" id="CHEBI:74443"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 10">
            <text>a 5,6-dihydrouridine in tRNA + NADP(+) = a uridine in tRNA + H(+) + NADPH</text>
            <dbReference type="Rhea" id="RHEA:23624"/>
            <dbReference type="Rhea" id="RHEA-COMP:13339"/>
            <dbReference type="Rhea" id="RHEA-COMP:13887"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="ChEBI" id="CHEBI:74443"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3 10 11">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the Dus family. DusB subfamily.</text>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the dus family.</text>
    </comment>
    <dbReference type="EC" id="1.3.1.-" evidence="10"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07315.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0429"/>
    <dbReference type="EnsemblBacteria" id="ABF07315">
        <property type="protein sequence ID" value="ABF07315"/>
        <property type="gene ID" value="Rmet_0429"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0429"/>
    <dbReference type="eggNOG" id="COG0042">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013299_0_3_4"/>
    <dbReference type="OMA" id="RPWLFAD"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017150">
        <property type="term" value="F:tRNA dihydrouridine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02801">
        <property type="entry name" value="DUS_like_FMN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1200.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02042">
        <property type="entry name" value="DusB_subfam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035587">
        <property type="entry name" value="DUS-like_FMN-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001269">
        <property type="entry name" value="DUS_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032887">
        <property type="entry name" value="DusB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024036">
        <property type="entry name" value="tRNA-dHydroUridine_Synthase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004652">
        <property type="entry name" value="tRNA_dU_NifR3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018517">
        <property type="entry name" value="tRNA_hU_synthase_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01207">
        <property type="entry name" value="Dus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006621">
        <property type="entry name" value="Dus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00737">
        <property type="entry name" value="nifR3_yhdG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01136">
        <property type="entry name" value="UPF0034"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 10 11" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="6 10 11" id="KW-0288">FMN</keyword>
    <keyword evidence="10" id="KW-0521">NADP</keyword>
    <keyword evidence="9 10 11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 10" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="7 10 11" id="KW-0819">tRNA processing</keyword>
    <keyword evidence="10" id="KW-0820">tRNA-binding</keyword>
    <feature type="domain" description="Dus" evidence="13">
        <location>
            <begin position="41"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="10 12">
        <location>
            <position position="127"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="43"/>
            <end position="45"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="97"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="168"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="229"/>
            <end position="231"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="253"/>
            <end position="254"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001183"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00001917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00002790"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRNR" id="PIRNR006621"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR006621-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF01207"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ABF07315.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="381" mass="41840" checksum="4F75A155DB7A5948" modified="2006-05-30" version="1">MRRPQGLAGACFCISPASPERTQHRSIVQIGPHQLRNNLFVAPMAGVTDRPFRQLCKQLGAGYAVSEMVASNAQLWKSEKTMRRANHAGEVEPISVQIAGAEPSMMAEAARYNVDRGAQIIDINMGCPAKKVCNVAAGSALLQNEPLVVRIVEAVVAAVGDRVPVTLKIRTGWDREHKNALRVARMVEDAGISMLTIHGRTRADLYHGEAEYETIAAVKSSIRIPVVANGDITTPQKARHVLAVTGADAIMIGRAAQGRPWLFREIEHYLKTGELLPSPEVAEIRAIMNHHLADHYAFYGEFTGVRTARKHIAWYTRGLKGANLFRHRMNTLESTDAQLAAVNAFFDEQAAISDRLVYVDENAAPDDDALDNNKKRELLAA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2007-11-13" modified="2022-08-03" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LRC6</accession>
    <name>ERPA_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Putative iron-sulfur cluster insertion protein ErpA</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">erpA</name>
        <name type="ordered locus">Rmet_0414</name>
    </gene>
    <organism>
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Required for insertion of 4Fe-4S clusters.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>iron-sulfur cluster</name>
            <dbReference type="ChEBI" id="CHEBI:30408"/>
        </cofactor>
        <text evidence="1">Binds 1 iron-sulfur cluster per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the HesB/IscA family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07300.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008646009.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q1LRC6"/>
    <dbReference type="SMR" id="Q1LRC6"/>
    <dbReference type="STRING" id="266264.Rmet_0414"/>
    <dbReference type="EnsemblBacteria" id="ABF07300">
        <property type="protein sequence ID" value="ABF07300"/>
        <property type="gene ID" value="Rmet_0414"/>
    </dbReference>
    <dbReference type="GeneID" id="60824016"/>
    <dbReference type="KEGG" id="rme:Rmet_0414"/>
    <dbReference type="eggNOG" id="COG0316">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069054_5_3_4"/>
    <dbReference type="OMA" id="YQLYFDE"/>
    <dbReference type="OrthoDB" id="1891602at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.300.12">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01380">
        <property type="entry name" value="Fe_S_insert_ErpA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000361">
        <property type="entry name" value="FeS_biogenesis"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016092">
        <property type="entry name" value="FeS_cluster_insertion"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017870">
        <property type="entry name" value="FeS_cluster_insertion_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023063">
        <property type="entry name" value="FeS_cluster_insertion_RrpA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035903">
        <property type="entry name" value="HesB-like_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01521">
        <property type="entry name" value="Fe-S_biosyn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89360">
        <property type="entry name" value="SSF89360"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00049">
        <property type="entry name" value="TIGR00049"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01152">
        <property type="entry name" value="HESB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0408">Iron</keyword>
    <keyword id="KW-0411">Iron-sulfur</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000311538" description="Putative iron-sulfur cluster insertion protein ErpA">
        <location>
            <begin position="1"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="50"/>
        </location>
        <ligand>
            <name>iron-sulfur cluster</name>
            <dbReference type="ChEBI" id="CHEBI:30408"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="114"/>
        </location>
        <ligand>
            <name>iron-sulfur cluster</name>
            <dbReference type="ChEBI" id="CHEBI:30408"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="116"/>
        </location>
        <ligand>
            <name>iron-sulfur cluster</name>
            <dbReference type="ChEBI" id="CHEBI:30408"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01380"/>
        </source>
    </evidence>
    <sequence length="122" mass="13095" checksum="A6ACF77E2CD880F2" modified="2006-05-30" version="1">MNAVAEAPATEDVPAPFVFTDSAADKVKQLIEEEGNAELKLRVFVQGGGCSGFQYGFTFDEDVNEDDTTMVKNGVTLLIDSMSYQYLVGAEIDYKEDINGAQFVIKNPNASTTCGCGSSFSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="53" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LRP2</accession>
    <name>Q1LRP2_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_0298</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07184.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0298"/>
    <dbReference type="EnsemblBacteria" id="ABF07184">
        <property type="protein sequence ID" value="ABF07184"/>
        <property type="gene ID" value="Rmet_0298"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0298"/>
    <dbReference type="eggNOG" id="ENOG50317F1">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_129289_0_0_4"/>
    <dbReference type="OMA" id="KMAACAK"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011690">
        <property type="entry name" value="P_starv_induced_PsiF"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07769">
        <property type="entry name" value="PsiF_repeat"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193731" evidence="2">
        <location>
            <begin position="32"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="36"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="57"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="96"/>
            <end position="115"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF07184.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="123" mass="12922" checksum="C42A5F710F24F44C" modified="2006-05-30" version="1">MMCFHSQERSMKKLIAMCVLVTPLFATGAFAQGASAPAAEKSGKAPTAQQEKMSACSKQNKGKKGDDYKKSMSDCLSGSAAATAPAAPKTQQEKMSACSKENKGKKGDDYKKSMSDCLSKPAA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-10-31" modified="2022-08-03" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LRP3</accession>
    <name>Y297_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Nucleotide-binding protein Rmet_0297</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">Rmet_0297</name>
    </gene>
    <organism>
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Displays ATPase and GTPase activities.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the RapZ-like family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07183.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515185.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q1LRP3"/>
    <dbReference type="SMR" id="Q1LRP3"/>
    <dbReference type="STRING" id="266264.Rmet_0297"/>
    <dbReference type="EnsemblBacteria" id="ABF07183">
        <property type="protein sequence ID" value="ABF07183"/>
        <property type="gene ID" value="Rmet_0297"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0297"/>
    <dbReference type="eggNOG" id="COG1660">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_059558_1_1_4"/>
    <dbReference type="OMA" id="TVMSFGF"/>
    <dbReference type="OrthoDB" id="1603998at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00636">
        <property type="entry name" value="RapZ_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005337">
        <property type="entry name" value="RapZ-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30448">
        <property type="entry name" value="PTHR30448"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03668">
        <property type="entry name" value="ATP_bind_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005052">
        <property type="entry name" value="P-loopkin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0342">GTP-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000258988" description="Nucleotide-binding protein Rmet_0297">
        <location>
            <begin position="1"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="8"/>
            <end position="15"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="57"/>
            <end position="60"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00636"/>
        </source>
    </evidence>
    <sequence length="296" mass="32856" checksum="F1CF32BFE004A3D7" modified="2006-05-30" version="1">MRIILITGISGSGKSVALNVLEDAGYYCVDNLPAQFIPDLACYLADQGYSQLGVATDIRSRESLARLPDTVRELAANHQVQVLYLTASTDALVQRYSETRRRHPLSAQVDVGAAAGTPNDTSLIEAIEKERELLSPLAESAHRIDTSNVRTNTLRSWIKELIQDENENKNSQQLTLLFESFGFKHGVPSDADLVFDVRSLPNPYYDLALRPLTGRDAPVIDFLQSQPMVLAMAEDIRAYVEKWLPSFIADNRSYLTVTIGCTGGQHRSVFIAERLANYFRAHGNVLVRHRELAPAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LS15</accession>
    <name>Q1LS15_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">5-formyltetrahydrofolate cyclo-ligase</fullName>
            <ecNumber evidence="2">6.3.3.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_0175</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>(6S)-5-formyl-5,6,7,8-tetrahydrofolate + ATP = 5,10-methenyltetrahydrofolate + ADP + phosphate</text>
            <dbReference type="Rhea" id="RHEA:10488"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57455"/>
            <dbReference type="ChEBI" id="CHEBI:57457"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.3.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the 5-formyltetrahydrofolate cyclo-ligase family.</text>
    </comment>
    <dbReference type="EC" id="6.3.3.2" evidence="2"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07061.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0175"/>
    <dbReference type="EnsemblBacteria" id="ABF07061">
        <property type="protein sequence ID" value="ABF07061"/>
        <property type="gene ID" value="Rmet_0175"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0175"/>
    <dbReference type="eggNOG" id="COG0212">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_066245_0_1_4"/>
    <dbReference type="OMA" id="IVHHEEN"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030272">
        <property type="term" value="F:5-formyltetrahydrofolate cyclo-ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10420">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002698">
        <property type="entry name" value="FTHF_cligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024185">
        <property type="entry name" value="FTHF_cligase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037171">
        <property type="entry name" value="NagB/RpiA_transferase-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01812">
        <property type="entry name" value="5-FTHF_cyc-lig"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100950">
        <property type="entry name" value="SSF100950"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02727">
        <property type="entry name" value="MTHFS_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0436">Ligase</keyword>
    <keyword evidence="2" id="KW-0460">Magnesium</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010638"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU361279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF07061.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="244" mass="26467" checksum="0080CCFF45EB8866" modified="2006-05-30" version="1">MLGARLSRDRHAGFVIRTTGAHLHLRVQHCFQMSVRWPLLHAMPPNQAPDNGPDSLYFTAPEDRRALRRRLLALRAALPDRETADAQIAAALAPLLERLGVQRLGFYWPIQQEFDARDTVARWLAAMPGRVAALPVVSRAAAPLDFHAWTPDTTMRVGQYGIPVPDGTGAVTPDALLIPCVGFSANKFRLGYGGGFYDRTLAAMAAANATPIAIGIGFAACEAPLQAASHDLPMDWLVTELGAR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-04-14" modified="2022-08-03" version="107" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LS20</accession>
    <name>SAHH_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Adenosylhomocysteinase</fullName>
            <ecNumber evidence="1">3.3.1.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">S-adenosyl-L-homocysteine hydrolase</fullName>
            <shortName evidence="1">AdoHcyase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">ahcY</name>
        <name type="ordered locus">Rmet_0170</name>
    </gene>
    <organism>
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">May play a key role in the regulation of the intracellular concentration of adenosylhomocysteine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>H2O + S-adenosyl-L-homocysteine = adenosine + L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:21708"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16335"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:58199"/>
            <dbReference type="EC" id="3.3.1.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </cofactor>
        <text evidence="1">Binds 1 NAD(+) per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-homocysteine biosynthesis; L-homocysteine from S-adenosyl-L-homocysteine: step 1/1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the adenosylhomocysteinase family.</text>
    </comment>
    <dbReference type="EC" id="3.3.1.1" evidence="1"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07056.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008641860.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q1LS20"/>
    <dbReference type="SMR" id="Q1LS20"/>
    <dbReference type="STRING" id="266264.Rmet_0170"/>
    <dbReference type="EnsemblBacteria" id="ABF07056">
        <property type="protein sequence ID" value="ABF07056"/>
        <property type="gene ID" value="Rmet_0170"/>
    </dbReference>
    <dbReference type="GeneID" id="60822893"/>
    <dbReference type="KEGG" id="rme:Rmet_0170"/>
    <dbReference type="eggNOG" id="COG0499">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025194_2_1_4"/>
    <dbReference type="OMA" id="NKYGCRE"/>
    <dbReference type="OrthoDB" id="522981at2"/>
    <dbReference type="UniPathway" id="UPA00314">
        <property type="reaction ID" value="UER00076"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004013">
        <property type="term" value="F:adenosylhomocysteinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006730">
        <property type="term" value="P:one-carbon metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00401">
        <property type="entry name" value="SAHH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1480">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00563">
        <property type="entry name" value="AdoHcyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042172">
        <property type="entry name" value="Adenosylhomocyst_ase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000043">
        <property type="entry name" value="Adenosylhomocysteinase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015878">
        <property type="entry name" value="Ado_hCys_hydrolase_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020082">
        <property type="entry name" value="S-Ado-L-homoCys_hydrolase_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23420">
        <property type="entry name" value="PTHR23420"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05221">
        <property type="entry name" value="AdoHcyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00670">
        <property type="entry name" value="AdoHcyase_NAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001109">
        <property type="entry name" value="Ad_hcy_hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00996">
        <property type="entry name" value="AdoHcyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00997">
        <property type="entry name" value="AdoHcyase_NAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00936">
        <property type="entry name" value="ahcY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00738">
        <property type="entry name" value="ADOHCYASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00739">
        <property type="entry name" value="ADOHCYASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0520">NAD</keyword>
    <keyword id="KW-0554">One-carbon metabolism</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_1000129299" description="Adenosylhomocysteinase">
        <location>
            <begin position="1"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="61"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="136"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="196"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="197"/>
            <end position="199"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="226"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="230"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="231"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="260"/>
            <end position="265"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="283"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="318"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="339"/>
            <end position="341"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="384"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00563"/>
        </source>
    </evidence>
    <sequence length="472" mass="51804" checksum="A31DF462B0F75F37" modified="2006-05-30" version="1">MNAVTDLKQDYIVADIGLAGWGRKEIAIAETEMPGLMAIRDEFAAAQPLKGARIAGSLHMTIQTAVLIETLKALGADVRWASCNIFSTQDHAAAAIAASGTPVFAFKGESLKEYWDFTHRIFDWADGGTPNMILDDGGDATLLLHLGARAEKDASLIAKPTSEEETFLFAAIKEKLAKDSTWYSRNLAAIRGVTEETTTGVHRLYQMAQKGELKFPAINVNDSVTKSKFDNLYGCRESLVDGIKRATDVMIAGKIAVVAGYGDVGKGSAQALRALSAQVWVTEIDPICALQAAMEGYRVVTMDYAAEHGDIFVTCTGNYHVITHDHMAKMKDQAIVCNIGHFDNEIDIASVEKYEWDEIKPQVDHVKFPDGKKIIILAKGRLVNLGCATGHPSYVMSSSFANQTIAQIELWAERDSGKYPVGVYVLPKHLDEKVARLQLRKLNAQLTELTEQQAAYIGVKKEGPYKADHYRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="65" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LS96</accession>
    <name>Q1LS96_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Rmet_0094</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF06980.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515015.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0094"/>
    <dbReference type="EnsemblBacteria" id="ABF06980">
        <property type="protein sequence ID" value="ABF06980"/>
        <property type="gene ID" value="Rmet_0094"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0094"/>
    <dbReference type="HOGENOM" id="CLU_1841799_0_0_4"/>
    <dbReference type="OMA" id="GMMRARE"/>
    <dbReference type="OrthoDB" id="1868133at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043605">
        <property type="entry name" value="DUF883_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043604">
        <property type="entry name" value="DUF883_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05957">
        <property type="entry name" value="DUF883"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19029">
        <property type="entry name" value="DUF883_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="119"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="domain" description="DUF883" evidence="2">
        <location>
            <begin position="44"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="domain" description="DUF883_C" evidence="3">
        <location>
            <begin position="111"/>
            <end position="141"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF05957"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF19029"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF06980.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="144" mass="15729" checksum="837A0537DD1DC221" modified="2006-05-30" version="1">MLTQNPKVRKEINHLHDSADAAVRQIRHAARDTRDAARDAAGPVSEDVKALIGQLQQTIDVLAREGSAESLAAGRRLRDRAQELAERLRERGRDGMDWARVHVDDAVDHSRQRVVESPLMAVGIAALVGAFVGLLLAGGRRSDD</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-01-15" modified="2022-05-25" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LSE4</accession>
    <name>GATB_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase subunit B</fullName>
            <shortName evidence="1">Asp/Glu-ADT subunit B</shortName>
            <ecNumber evidence="1">6.3.5.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">gatB</name>
        <name type="ordered locus">Rmet_0046</name>
    </gene>
    <organism>
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp-tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases. The reaction takes place in the presence of glutamine and ATP through an activated phospho-Asp-tRNA(Asn) or phospho-Glu-tRNA(Gln).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + H2O + L-glutamine + L-glutamyl-tRNA(Gln) = ADP + H(+) + L-glutamate + L-glutaminyl-tRNA(Gln) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:17521"/>
            <dbReference type="Rhea" id="RHEA-COMP:9681"/>
            <dbReference type="Rhea" id="RHEA-COMP:9684"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:78520"/>
            <dbReference type="ChEBI" id="CHEBI:78521"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + H2O + L-aspartyl-tRNA(Asn) + L-glutamine = ADP + 2 H(+) + L-asparaginyl-tRNA(Asn) + L-glutamate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:14513"/>
            <dbReference type="Rhea" id="RHEA-COMP:9674"/>
            <dbReference type="Rhea" id="RHEA-COMP:9677"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:78515"/>
            <dbReference type="ChEBI" id="CHEBI:78516"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Heterotrimer of A, B and C subunits.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the GatB/GatE family. GatB subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.3.5.-" evidence="1"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF06932.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011514973.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q1LSE4"/>
    <dbReference type="SMR" id="Q1LSE4"/>
    <dbReference type="STRING" id="266264.Rmet_0046"/>
    <dbReference type="EnsemblBacteria" id="ABF06932">
        <property type="protein sequence ID" value="ABF06932"/>
        <property type="gene ID" value="Rmet_0046"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0046"/>
    <dbReference type="eggNOG" id="COG0064">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019240_0_0_4"/>
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    <dbReference type="OrthoDB" id="497127at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050566">
        <property type="term" value="F:asparaginyl-tRNA synthase (glutamine-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
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        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="F:glutaminyl-tRNA synthase (glutamine-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
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        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
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        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00121">
        <property type="entry name" value="GatB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004413">
        <property type="entry name" value="Apn/Gln-ADT_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017959">
        <property type="entry name" value="Asn/Gln-tRNA_amidoTrfase_suB/E"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006075">
        <property type="entry name" value="Asn/Gln-tRNA_Trfase_suB/E_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018027">
        <property type="entry name" value="Asn/Gln_amidotransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003789">
        <property type="entry name" value="Asn/Gln_tRNA_amidoTrase-B-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042114">
        <property type="entry name" value="GatB_C_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023168">
        <property type="entry name" value="GatB_Yqey_C_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017958">
        <property type="entry name" value="Gln-tRNA_amidoTrfase_suB_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014746">
        <property type="entry name" value="Gln_synth/guanido_kin_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11659">
        <property type="entry name" value="PTHR11659"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02934">
        <property type="entry name" value="GatB_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02637">
        <property type="entry name" value="GatB_Yqey"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00845">
        <property type="entry name" value="GatB_Yqey"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55931">
        <property type="entry name" value="SSF55931"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89095">
        <property type="entry name" value="SSF89095"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="gatB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01234">
        <property type="entry name" value="GATB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_1000016027" description="Aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase subunit B">
        <location>
            <begin position="1"/>
            <end position="484"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00121"/>
        </source>
    </evidence>
    <sequence length="484" mass="52574" checksum="90CC99F7090E9C45" modified="2006-05-30" version="1">MQWEVVIGLETHTQLSTASKIFSGTSTAFGAEPNTQASPVDLALPGVLPVLNRGAVERAIQFGLSIGATIAPRSIFARKNYFYPDLPKGYQISQYEIPVVQGGQITIQVEGKQGVYEKTVNLTRAHLEEDAGKSLHEDFAGMTGIDLNRAGTPLLEIVTEPDMRSSAEAVAYAKALHSLVVWLGICDGNMQEGSFRCDANVSVRPVGQEKFGTRREIKNLNSFRFLQQAIDYEVQWQIAEIEDGREIVQATVLFDPDTGETRAMRTKEDAHDYRYFPDPDLMPLEIDAAWVERVKSELPELPATMQARFVSQYGLSAYDASTITATKALAAYYEAVVTNVGAASAKPAANWVMGEIASQLNREGISIDEAPVRPAQLAKLLARITDGTVSNNTAKKDVFPAMWAGESDGDADAIIAAKGLKQMSDTGELEKIIDDVLAANAKSVEEFRAGKEKAFNALVGQAMKATKGKANPAQVNELLKKKLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LSH3</accession>
    <name>Q1LSH3_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">MFS transporter, DHA2 family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Rmet_0017</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF06903.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0017"/>
    <dbReference type="EnsemblBacteria" id="ABF06903">
        <property type="protein sequence ID" value="ABF06903"/>
        <property type="gene ID" value="Rmet_0017"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0017"/>
    <dbReference type="eggNOG" id="COG0477">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000960_2_7_4"/>
    <dbReference type="OMA" id="GWNFLVQ"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="SSF103473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="35"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="69"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="100"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="125"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="158"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="188"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="220"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="245"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="289"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="326"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="355"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="381"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="417"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="487"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="domain" description="MFS" evidence="6">
        <location>
            <begin position="35"/>
            <end position="513"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABF06903.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="528" mass="55393" checksum="F7F32DDC734D8471" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2005-04-26" modified="2022-05-25" version="139" xmlns="http://uniprot.org/uniprot">
    <accession>Q58AD5</accession>
    <name>Q58AD5_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">CopR regulator from the two components sensor/regulator family involved in Cu(II)/Cu(I) resistance</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">copR1</name>
        <name evidence="6" type="ordered locus">Rmet_6111</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="6 7">
        <name>pMOL30</name>
    </geneLocation>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="7">Plasmid pMOL30</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000354">
        <property type="protein sequence ID" value="ABF12970.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008652575.1">
        <property type="nucleotide sequence ID" value="NC_007971.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_145683.1">
        <property type="nucleotide sequence ID" value="NC_006466.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF12970">
        <property type="protein sequence ID" value="ABF12970"/>
        <property type="gene ID" value="Rmet_6111"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6111"/>
    <dbReference type="HOGENOM" id="CLU_000445_30_1_4"/>
    <dbReference type="OMA" id="LWIDLIS"/>
    <dbReference type="OrthoDB" id="1020672at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid pMOL30"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00383">
        <property type="entry name" value="trans_reg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001867">
        <property type="entry name" value="OmpR/PhoB-type_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006291">
        <property type="entry name" value="PcoR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016032">
        <property type="entry name" value="Sig_transdc_resp-reg_C-effctor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039420">
        <property type="entry name" value="WalR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48111">
        <property type="entry name" value="PTHR48111"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00486">
        <property type="entry name" value="Trans_reg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00862">
        <property type="entry name" value="Trans_reg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46894">
        <property type="entry name" value="SSF46894"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01387">
        <property type="entry name" value="cztR_silR_copR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51755">
        <property type="entry name" value="OMPR_PHOB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="6" id="KW-0614">Plasmid</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Response regulatory" evidence="4">
        <location>
            <begin position="2"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="domain" description="OmpR/PhoB-type" evidence="5">
        <location>
            <begin position="125"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="OmpR/PhoB-type" evidence="3">
        <location>
            <begin position="125"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="2">
        <location>
            <position position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABF12970.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="228" mass="25526" checksum="0210784E91B5C153" modified="2005-04-26" version="1">MKLLVVEDEVKTGEYLRQGLTEAGFVVDLVANGLDGQHFAVNETYDLIILDVMLPDVDGWHILHAIRASGNAVPVLFLTARDSVADRVRGLELGADDYLVKPFAFSELLARVRTLLRRGAVQLAMDRIQVADLILDLSRRRASRGGRRITLTSKEFALLELFARRRGEVLPRSLIASQVWDMNFDSDSNVIDVAIRRLRAKIDDGFEVKLIQTVRGMGYVLEAPEDPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-04-26" modified="2021-09-29" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>Q58AE4</accession>
    <name>Q58AE4_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Type II site-specific deoxyribonuclease (Type II restriction enzyme) involved in resistance to copper (Cu(I)/Cu(II))</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">copL</name>
        <name evidence="2" type="ordered locus">Rmet_6120</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2 3">
        <name>pMOL30</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid pMOL30</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000354">
        <property type="protein sequence ID" value="ABF12979.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008652084.1">
        <property type="nucleotide sequence ID" value="NC_007971.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_145674.1">
        <property type="nucleotide sequence ID" value="NC_006466.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF12979">
        <property type="protein sequence ID" value="ABF12979"/>
        <property type="gene ID" value="Rmet_6120"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6120"/>
    <dbReference type="HOGENOM" id="CLU_647015_0_0_4"/>
    <dbReference type="OMA" id="WIAANDR"/>
    <dbReference type="OrthoDB" id="334459at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid pMOL30"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="226"/>
            <end position="253"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF12979.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="421" mass="46453" checksum="04292197CD7C4D03" modified="2005-04-26" version="1">MTDTTRTLIERWKSDPSGTYQTWFLWPKRLKNFRSIRRGIGAVVEEIHAGTFGNAYRGSSLETVVASVAEQRQIFKGADHAFLWKPKLRIPDIYENADNQRAFARLLHTCNCCDTEAEMLTAIQRLSGVGIKGLGPAVANLLYFIHPTLLPPFNTAIVNGYNALTGAAVKLGKWDHYLAMRDRILALNHRYRDVLSNDLGALAGLLFDVGTGRYPAPPCNDLPGEVSAWEDDLAKVREQASALAKQIQADKLEDATHTEVQGWLRDLGKSLGYAVWIATNDRGREYNGEPLSLGCLSALPATLLVSGGETVPLIDAVWLDPATLEVAAAFEVEHSTSIYSGIVRLLDLALGTSQSATKHLFLVAPDNREAEVRAQLSRPAFSRVAELNMRYVPYSSLREHRENIARFGMGIKPIEAIAKSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-04-26" modified="2021-06-02" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>Q58AM5</accession>
    <name>Q58AM5_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Protein MgtC</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">czcM</name>
        <name evidence="8" type="ordered locus">Rmet_5985</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="8 9">
        <name>pMOL30</name>
    </geneLocation>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="9">Plasmid pMOL30</plasmid>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cell inner membrane</location>
            <topology evidence="7">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the MgtC/SapB family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000354">
        <property type="protein sequence ID" value="ABF12844.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011229343.1">
        <property type="nucleotide sequence ID" value="NC_007971.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_145590.1">
        <property type="nucleotide sequence ID" value="NC_006466.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF12844">
        <property type="protein sequence ID" value="ABF12844"/>
        <property type="gene ID" value="Rmet_5985"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5985"/>
    <dbReference type="HOGENOM" id="CLU_079292_1_1_4"/>
    <dbReference type="OMA" id="AANLWLT"/>
    <dbReference type="OrthoDB" id="2022224at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid pMOL30"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003416">
        <property type="entry name" value="MgtC/SapB/SrpB/YhiD_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33778">
        <property type="entry name" value="PTHR33778"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02308">
        <property type="entry name" value="MgtC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01837">
        <property type="entry name" value="MGTCSAPBPROT"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0614">Plasmid</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="21"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="52"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="81"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="113"/>
            <end position="144"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009298"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU365041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ABF12844.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="152" mass="15630" checksum="411E84DC7B7B77B1" modified="2005-04-26" version="1">MNDIIRTLGSEFSDLANAAEAVRAVVRMTVSIVLGGLLGYERESSGKSAGVRTHMLVALGACVFVVVPLQAGVQLADMSRVLQGLTSGIGFLCAGAILKPDNETHVRGLTTAASIWIAAAIGVAAGMGHAVTAIVATAFALIVLRILQMSKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-04-26" modified="2022-08-03" version="121" xmlns="http://uniprot.org/uniprot">
    <accession>Q58AN1</accession>
    <name>Q58AN1_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Mercuric resistance operon regulatory protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">merR</name>
        <name evidence="11" type="ordered locus">Rmet_5990</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="11 12">
        <name>pMOL30</name>
    </geneLocation>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="12">Plasmid pMOL30</plasmid>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Mediates the mercuric-dependent induction of mercury resistance operon. In the absence of mercury MerR represses transcription by binding tightly to the mer operator region; when mercury is present the dimeric complex binds a single ion and becomes a potent transcriptional activator, while remaining bound to the mer site.</text>
    </comment>
    <dbReference type="EMBL" id="CP000354">
        <property type="protein sequence ID" value="ABF12849.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011229338.1">
        <property type="nucleotide sequence ID" value="NC_007971.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_145584.1">
        <property type="nucleotide sequence ID" value="NC_006466.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF12849">
        <property type="protein sequence ID" value="ABF12849"/>
        <property type="gene ID" value="Rmet_5990"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5990"/>
    <dbReference type="HOGENOM" id="CLU_060077_2_0_4"/>
    <dbReference type="OMA" id="HNRSAAR"/>
    <dbReference type="OrthoDB" id="1748559at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid pMOL30"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045340">
        <property type="term" value="F:mercury ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046689">
        <property type="term" value="P:response to mercury ion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04783">
        <property type="entry name" value="HTH_MerR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009061">
        <property type="entry name" value="DNA-bd_dom_put_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011794">
        <property type="entry name" value="MerR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000551">
        <property type="entry name" value="MerR-type_HTH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015358">
        <property type="entry name" value="Tscrpt_reg_MerR_DNA-bd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00376">
        <property type="entry name" value="MerR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09278">
        <property type="entry name" value="MerR-DNA-bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00040">
        <property type="entry name" value="HTHMERR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00422">
        <property type="entry name" value="HTH_MERR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46955">
        <property type="entry name" value="SSF46955"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02051">
        <property type="entry name" value="MerR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00552">
        <property type="entry name" value="HTH_MERR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50937">
        <property type="entry name" value="HTH_MERR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0010">Activator</keyword>
    <keyword evidence="6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0475">Mercuric resistance</keyword>
    <keyword evidence="4" id="KW-0476">Mercury</keyword>
    <keyword evidence="11" id="KW-0614">Plasmid</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0678">Repressor</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH merR-type" evidence="10">
        <location>
            <begin position="4"/>
            <end position="73"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00017146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00024874"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ABF12849.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="132" mass="14306" checksum="BE5F455538341E6E" modified="2005-04-26" version="1">MDAELTIGKLAEAAGVNIETIRYYQRRGLLDEPPKPLGGQRRYSAELAGRVRFIKRAQALGFTLDEVSALLALDSACACSKTRELASRKLALIKQKMADLATMQQVLDALIQQCDAGSVGADCPIIDVLARD</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-02-01" modified="2021-06-02" version="65" xmlns="http://uniprot.org/uniprot">
    <accession>Q5LK38</accession>
    <name>Q5LK38_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_6267</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="1 2">
        <name>pMOL28</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid pMOL28</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000355">
        <property type="protein sequence ID" value="ABF13126.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008648655.1">
        <property type="nucleotide sequence ID" value="NC_007972.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_007391655.1">
        <property type="nucleotide sequence ID" value="NC_006525.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF13126">
        <property type="protein sequence ID" value="ABF13126"/>
        <property type="gene ID" value="Rmet_6267"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6267"/>
    <dbReference type="HOGENOM" id="CLU_1388230_0_0_4"/>
    <dbReference type="OMA" id="AIWNSRV"/>
    <dbReference type="OrthoDB" id="1383358at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid pMOL28"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF13126.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="196" mass="21490" checksum="75E48EA096E7DBD5" modified="2005-02-01" version="1">MPSGLLQCAHCDGAPTYISGRLQAVIVCEECGISTPPVRLDSADKDTAFTTLSAIWNSRVEHRPADQNAVSMMSRRALTASDIPYFLNLLAATTSDWETVGPKFAAMAAALAQPGYEFKHVDPPETVISQAARYQKLLKRAKIIYIDGAPMIRFDHVPALCAEIAEESLADKDWPLLDLHEFIGKAIDGLPDHWQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-01-04" modified="2021-09-29" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>Q5NV07</accession>
    <name>Q5NV07_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Conjugal transfer protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">pilL</name>
        <name evidence="2" type="ordered locus">Rmet_6297</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2 3">
        <name>pMOL28</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid pMOL28</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000355">
        <property type="protein sequence ID" value="ABF13156.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011239903.1">
        <property type="nucleotide sequence ID" value="NC_007972.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_161643.1">
        <property type="nucleotide sequence ID" value="NC_006525.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF13156">
        <property type="protein sequence ID" value="ABF13156"/>
        <property type="gene ID" value="Rmet_6297"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6297"/>
    <dbReference type="HOGENOM" id="CLU_054158_0_0_4"/>
    <dbReference type="OMA" id="ENWMQAL"/>
    <dbReference type="OrthoDB" id="729198at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid pMOL28"/>
    </dbReference>
    <dbReference type="CDD" id="cd06911">
        <property type="entry name" value="VirB9_CagX_TrbG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033645">
        <property type="entry name" value="VirB9/CagX/TrbG_C"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5013532126" evidence="1">
        <location>
            <begin position="20"/>
            <end position="408"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF13156.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="408" mass="44116" checksum="FBAA39518A5AD9E3" modified="2005-01-04" version="1">MIRPLLFSAVALVAACAHAADTDPLDFDYQVVARAADRPAMVFNDGLSTYIQPRHGQLVQADGAIQNGPYWVIDGVPDVLRYTVNGQPVVARWKRANGFTSEPANPSGDMPRSLASVSGRLALIGNYADLPLVRVGRSSLPLAQMVKSIAPAGWSGTAQKDIPLTDDVSLDAKAGENWVQALARVLEQRNLYAEVDFVRRNISLRATPPKAFSVEPGHAAQTAGMLRVAAPVADAAPTATPLPEGPTLASAFNALAIRDAKNGRIEVRFESEPKDLELRDGSGDKLRTRWDDTEKVLSFPTVDNFTASGGGKRVEVARVPGINFLFPRDNSAGLERVFEKDGATYLSFAKSMASISVFGDNHQGGGEQKDRYYKFNGVSDHLTVIADGNIVRVDRLPEVRFYERAAAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-03-23" modified="2021-06-02" version="51" xmlns="http://uniprot.org/uniprot">
    <accession>D3DXK6</accession>
    <name>D3DXK6_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative rhodanese-related sulfurtransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_6408</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ADC45026.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515147.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_6408"/>
    <dbReference type="EnsemblBacteria" id="ADC45026">
        <property type="protein sequence ID" value="ADC45026"/>
        <property type="gene ID" value="Rmet_6408"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6408"/>
    <dbReference type="eggNOG" id="COG0607">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_089574_1_5_4"/>
    <dbReference type="OrthoDB" id="2056793at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.250.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001763">
        <property type="entry name" value="Rhodanese-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036873">
        <property type="entry name" value="Rhodanese-like_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00581">
        <property type="entry name" value="Rhodanese"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00450">
        <property type="entry name" value="RHOD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52821">
        <property type="entry name" value="SSF52821"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50206">
        <property type="entry name" value="RHODANESE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Rhodanese" evidence="1">
        <location>
            <begin position="47"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADC45026.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="137" mass="14287" checksum="27F39CE9D2476A0D" modified="2010-03-23" version="1">MNFFADYNNLALIAIAVVSGGLLAWPTIKSGTGGKRVNAAAATQLINKRNAVVVDIRDAAEFAKGHMPQAKSAPLDDLPNRAGGLAKDKAIPIIVVCQNGQRSSKAQAALKEAGYSEVYALEGGMAAWQQAGLPVIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-03-23" modified="2020-08-12" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>D3DXP2</accession>
    <name>D3DXP2_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Transposase (OrfA) ISRme6</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">tnpA</name>
        <name evidence="1" type="ordered locus">Rmet_1309</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ADC45062.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1309"/>
    <dbReference type="EnsemblBacteria" id="ADC45062">
        <property type="protein sequence ID" value="ADC45062"/>
        <property type="gene ID" value="Rmet_1309"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1309"/>
    <dbReference type="eggNOG" id="COG3293">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_055261_4_2_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025161">
        <property type="entry name" value="Put_IS4/IS5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13340">
        <property type="entry name" value="DUF4096"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADC45062.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="136" mass="15118" checksum="E18B99DA4E50E32B" modified="2010-03-23" version="1">MEAPIIDDELWTLIEPLLPPPKPRRKGHPGRPRVSDRAALNGILFVLKTGLRWNHLPTVLGFGSGATCWRRLNDWRKAGVWDRLHELLLDKLREAGQIDLSYAAVDSSSVRAVGAGEKLARTPRIARDPVPNTTSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-03-23" modified="2022-01-19" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>D3DXW0</accession>
    <name>D3DXW0_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_6523</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ADC45130.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_003153638.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_6523"/>
    <dbReference type="EnsemblBacteria" id="ADC45130">
        <property type="protein sequence ID" value="ADC45130"/>
        <property type="gene ID" value="Rmet_6523"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6523"/>
    <dbReference type="eggNOG" id="ENOG502Z9RP">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1685278_0_0_4"/>
    <dbReference type="OrthoDB" id="1750660at2"/>
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        <property type="component" value="Chromosome"/>
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    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADC45130.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="156" mass="16700" checksum="E82972E41C1C2B4F" modified="2010-03-23" version="1">MTTVVSRTFRSSPHRDALQTWDAIVELLTQGKDGTARSELRAVTGVAASLIADQAPKSAPIVATCDGPRTRIYCLFDEDAIYGDDANEEVLGFEPLKGDWGVSLPCPKEQLGWVQSALKKHSSRIIARDLSQGIATQAQADAGQALSLDLGGFLKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-03-23" modified="2020-10-07" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>D3DXY7</accession>
    <name>D3DXY7_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_6550</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ADC45157.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_6550"/>
    <dbReference type="EnsemblBacteria" id="ADC45157">
        <property type="protein sequence ID" value="ADC45157"/>
        <property type="gene ID" value="Rmet_6550"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6550"/>
    <dbReference type="HOGENOM" id="CLU_3358005_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADC45157.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="36" mass="4334" checksum="06C350CE905058F5" modified="2010-03-23" version="1">MDRSVDPKAEKQKKDEELTFAQFANEYYLPHAEIYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-03-23" modified="2020-10-07" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>D3DY34</accession>
    <name>D3DY34_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_6603</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ADC45204.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_6603"/>
    <dbReference type="EnsemblBacteria" id="ADC45204">
        <property type="protein sequence ID" value="ADC45204"/>
        <property type="gene ID" value="Rmet_6603"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6603"/>
    <dbReference type="HOGENOM" id="CLU_2571332_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="61"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADC45204.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="81" mass="8244" checksum="72F5BF388DD1536A" modified="2010-03-23" version="1">MKRPAATGGGQRGRAGWRPPTIAWKIASWMSAAPGLFSSDPDLVVQVVSASGSEISEIRVDRGAPIASDRAKGGAPGQSGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-03-23" modified="2020-10-07" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>D3DY45</accession>
    <name>D3DY45_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_6616</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ADC45215.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_6616"/>
    <dbReference type="EnsemblBacteria" id="ADC45215">
        <property type="protein sequence ID" value="ADC45215"/>
        <property type="gene ID" value="Rmet_6616"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6616"/>
    <dbReference type="HOGENOM" id="CLU_3383437_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADC45215.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="33" mass="3724" checksum="0565AAEDD1743F2D" modified="2010-03-23" version="1">MIAFPHQGLCTRVAHLISIGSMQILYCEQFNCV</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1998-12-15" modified="2022-05-25" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>P56621</accession>
    <accession>Q5NUX0</accession>
    <accession>Q9L3G1</accession>
    <name>CNRY_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName>Nickel and cobalt resistance protein CnrY</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">cnrY</name>
        <name type="synonym">ORF0b</name>
        <name type="ordered locus">Rmet_6205</name>
        <name type="ORF">RMe0088</name>
    </gene>
    <organism>
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid">
        <name>pMOL28</name>
    </geneLocation>
    <reference key="1">
        <citation type="journal article" date="1993" name="J. Bacteriol." volume="175" first="767" last="778">
            <title>Characterization of the inducible nickel and cobalt resistance determinant cnr from pMOL28 of Alcaligenes eutrophus CH34.</title>
            <authorList>
                <person name="Liesegang H."/>
                <person name="Lemke K."/>
                <person name="Siddiqui R.A."/>
                <person name="Schlegel H.G."/>
            </authorList>
            <dbReference type="PubMed" id="8380802"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC DNA]</scope>
    </reference>
    <reference key="2">
        <citation type="submission" date="2004-10" db="EMBL/GenBank/DDBJ databases">
            <title>Sequence and features of the Ralstonia metallidurans CH34 heavy metal plasmids pMOL28 and pMOL30.</title>
            <authorList>
                <person name="van der Lelie D."/>
                <person name="Monchy S."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="Benotmane M."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Mergeay M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference key="3">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <reference key="4">
        <citation type="journal article" date="2000" name="J. Bacteriol." volume="182" first="1390" last="1398">
            <title>Regulation of the cnr cobalt and nickel resistance determinant from Ralstonia sp. strain CH34.</title>
            <authorList>
                <person name="Grass G."/>
                <person name="Grosse C."/>
                <person name="Nies D.H."/>
            </authorList>
            <dbReference type="PubMed" id="10671463"/>
            <dbReference type="DOI" id="10.1128/JB.182.5.1390-1398.2000"/>
        </citation>
        <scope>CHARACTERIZATION</scope>
        <scope>INDUCTION</scope>
        <scope>SUBCELLULAR LOCATION</scope>
    </reference>
    <reference key="5">
        <citation type="journal article" date="2000" name="J. Bacteriol." volume="182" first="1399" last="1409">
            <title>Regulation of the cnr cobalt and nickel resistance determinant of Ralstonia eutropha (Alcaligenes eutrophus) CH34.</title>
            <authorList>
                <person name="Tibazarwa C."/>
                <person name="Wuertz S."/>
                <person name="Mergeay M."/>
                <person name="Wyns L."/>
                <person name="van Der Lelie D."/>
            </authorList>
            <dbReference type="PubMed" id="10671464"/>
            <dbReference type="DOI" id="10.1128/JB.182.5.1399-1409.2000"/>
        </citation>
        <scope>CHARACTERIZATION</scope>
        <scope>INDUCTION</scope>
    </reference>
    <comment type="function">
        <text>Nickel and cobalt resistance proteins CnrA, CnrB, CnrC CnrH and CnrR may be involved in the regulation of CNR.</text>
    </comment>
    <comment type="function">
        <text evidence="2 3">CnrH alone is able to activate cnr expression, and both CnrY and CrnX are needed for nickel induction of CnrH (PubMed:10671463). In the absence of wild-type CnrY (due either to a frameshift, PubMed:10671463 or absence of the transcript, PubMed:10671464), nickel and cobalt resistance is constitutive, indicating that CrnY may act as a repressor (PubMed:10671463) or an anti-sigma factor (PubMed:10671464).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell inner membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="induction">
        <text evidence="2 3">By nickel and cobalt.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">To A.xylosoxydans NccY.</text>
    </comment>
    <dbReference type="EMBL" id="M91650">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AJ276513">
        <property type="protein sequence ID" value="CAB82448.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="X90708">
        <property type="protein sequence ID" value="CAI30232.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP000355">
        <property type="protein sequence ID" value="ABF13064.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B47056">
        <property type="entry name" value="B47056"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011239971.1">
        <property type="nucleotide sequence ID" value="NC_007972.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_161710.1">
        <property type="nucleotide sequence ID" value="NC_006525.1"/>
    </dbReference>
    <dbReference type="PDB" id="4CXF">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="1.75 A"/>
        <property type="chains" value="B=1-95"/>
    </dbReference>
    <dbReference type="PDBsum" id="4CXF"/>
    <dbReference type="AlphaFoldDB" id="P56621"/>
    <dbReference type="SMR" id="P56621"/>
    <dbReference type="EnsemblBacteria" id="ABF13064">
        <property type="protein sequence ID" value="ABF13064"/>
        <property type="gene ID" value="Rmet_6205"/>
    </dbReference>
    <dbReference type="GeneID" id="60825777"/>
    <dbReference type="KEGG" id="rme:Rmet_6205"/>
    <dbReference type="HOGENOM" id="CLU_2370740_0_0_4"/>
    <dbReference type="OrthoDB" id="2070039at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid pMOL28"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="DisProt" id="DP01181"/>
    <dbReference type="InterPro" id="IPR035230">
        <property type="entry name" value="CnrY"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17524">
        <property type="entry name" value="CnrY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0997">Cell inner membrane</keyword>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0170">Cobalt</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-0533">Nickel</keyword>
    <keyword id="KW-0614">Plasmid</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000089978" description="Nickel and cobalt resistance protein CnrY">
        <location>
            <begin position="1"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="topological domain" description="Cytoplasmic">
        <location>
            <begin position="1"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="46"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="topological domain" description="Periplasmic">
        <location>
            <begin position="69"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="sequence conflict" description="In Ref. 1; M91650." evidence="4" ref="1">
        <original>R</original>
        <variation>RR</variation>
        <location>
            <position position="44"/>
        </location>
    </feature>
    <feature type="helix" evidence="5">
        <location>
            <begin position="4"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="helix" evidence="5">
        <location>
            <begin position="24"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000269" key="2">
        <source>
            <dbReference type="PubMed" id="10671463"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="3">
        <source>
            <dbReference type="PubMed" id="10671464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="4"/>
    <evidence type="ECO:0007829" key="5">
        <source>
            <dbReference type="PDB" id="4CXF"/>
        </source>
    </evidence>
    <sequence length="95" mass="10151" checksum="1DE90E9720849CD4" modified="2006-07-11" version="2">MADVEEWLTHARKVTQEASIGVDVTSIQECISAEPAQRVLVARRDAWRAICCAAFAALVAFAAINRVATIMLEKPAPTWVATPSAASPFGLLIGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>Q1L9P6</accession>
    <name>Q1L9P6_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">HEPN_AbiU2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_6271</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2 3">
        <name>pMOL28</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid pMOL28</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000355">
        <property type="protein sequence ID" value="ABF13130.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011514905.1">
        <property type="nucleotide sequence ID" value="NC_007972.2"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF13130">
        <property type="protein sequence ID" value="ABF13130"/>
        <property type="gene ID" value="Rmet_6271"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6271"/>
    <dbReference type="HOGENOM" id="CLU_095974_0_0_4"/>
    <dbReference type="OMA" id="LMISMER"/>
    <dbReference type="OrthoDB" id="2069346at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid pMOL28"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040704">
        <property type="entry name" value="HEPN_AbiU2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18734">
        <property type="entry name" value="HEPN_AbiU2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HEPN_AbiU2" evidence="1">
        <location>
            <begin position="6"/>
            <end position="199"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF18734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF13130.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="248" mass="28494" checksum="3C45432AA09391CC" modified="2006-05-30" version="1">MKPDYSFENQLKSLEHEANVIANYTYAEMTIQHAASQSKKLLDHLNETPTFWISCTAALQSAAYIALGRVFDQKSPYNLEALMISMERDLTIFGRDALAHRKRNGATSDPAWLDAYLSAAHYPTKADVARLRRNIEKYRCVYERAIMPVRHQYLAHRQAHGNTKVQALFARGKVADLWRLSTFLVRLHSALWGLLHNGRKPLLTPVRFSVNRIYADSSRNSRPHEQIVRETRALMKALEQLPKSSFRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LA18</accession>
    <name>Q1LA18_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Extra-cytoplasmic solute receptor protein Bug family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">bug</name>
        <name evidence="3" type="ordered locus">Rmet_6149</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3 4">
        <name>pMOL30</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid pMOL30</plasmid>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Periplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the UPF0065 (bug) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000354">
        <property type="protein sequence ID" value="ABF13008.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011514836.1">
        <property type="nucleotide sequence ID" value="NC_007971.2"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF13008">
        <property type="protein sequence ID" value="ABF13008"/>
        <property type="gene ID" value="Rmet_6149"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6149"/>
    <dbReference type="HOGENOM" id="CLU_045683_0_2_4"/>
    <dbReference type="OMA" id="INRRRFC"/>
    <dbReference type="OrthoDB" id="447734at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid pMOL30"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042597">
        <property type="term" value="C:periplasmic space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005064">
        <property type="entry name" value="BUG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042100">
        <property type="entry name" value="Bug_dom1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006311">
        <property type="entry name" value="TAT_signal"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42928">
        <property type="entry name" value="PTHR42928"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03401">
        <property type="entry name" value="TctC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017082">
        <property type="entry name" value="YflP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51318">
        <property type="entry name" value="TAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-0675">Receptor</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF13008.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="339" mass="35177" checksum="81BB62BEA8516A8A" modified="2006-05-30" version="1">MFSKETTLTQVDRSEFCLSRRAVLRGAAAAVAAAALPAPAQGAWPSRPVRIVVPFAAGGGSDAIARFTASRLNAALKQPFVVDNRPGAGGNLGTEQGLHSAPDGYTLTLIASSYSVNAAVYNLGFDPVADMTPIVQISNGPLLIVANPKTNIKTIGDLIQKAKTNPDKINYATSGAGGVAHAATELFMDRVGIRMTHIPYKGAAPAMSDTLAGTCDVYFSSVAIALPHIRAGKLVAVAVTTPTRLNALPNVPTVAESGIPGFDVPHWHGLIGPKGLSQEVVAKVNSAVNAFLKAPDANEHLQRDGVSAAGGTPSLLQERIAREVAGWKKLAAKGRLKVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-10-07" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LA92</accession>
    <name>Q1LA92_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_6075</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="1 2">
        <name>pMOL30</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid pMOL30</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000354">
        <property type="protein sequence ID" value="ABF12934.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF12934">
        <property type="protein sequence ID" value="ABF12934"/>
        <property type="gene ID" value="Rmet_6075"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6075"/>
    <dbReference type="HOGENOM" id="CLU_2438617_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid pMOL30"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF12934.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="90" mass="10329" checksum="405B8248232AF309" modified="2006-05-30" version="1">MDRYMDRANCVVWNLPCIGQHDVDVEDDCSIYNAFCFTLEYPYQDVPFVTLDVEGSYKQVAINLSAVNYVSVPTQRYNDGCAEADEEDDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="49" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LAB8</accession>
    <name>Q1LAB8_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative membrane protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_6049</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2 3">
        <name>pMOL30</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid pMOL30</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000354">
        <property type="protein sequence ID" value="ABF12908.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011514782.1">
        <property type="nucleotide sequence ID" value="NC_007971.2"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF12908">
        <property type="protein sequence ID" value="ABF12908"/>
        <property type="gene ID" value="Rmet_6049"/>
    </dbReference>
    <dbReference type="GeneID" id="60824691"/>
    <dbReference type="KEGG" id="rme:Rmet_6049"/>
    <dbReference type="HOGENOM" id="CLU_2452430_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid pMOL30"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="32"/>
            <end position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF12908.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="89" mass="9685" checksum="A4C551A169071DDE" modified="2006-05-30" version="1">MRTKVKVGLMIGGVVLVAAAIVLAPVLRDLPWLKWTLYVCAALMLSPLFMGGLVDVPATTPEDPTFEKNRIGDARQDVWGFNGQDSNND</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LAR2</accession>
    <name>Q1LAR2_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Transcriptional regulator, LysR-family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_5905</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the LysR transcriptional regulatory family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12764.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518112.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5905"/>
    <dbReference type="EnsemblBacteria" id="ABF12764">
        <property type="protein sequence ID" value="ABF12764"/>
        <property type="gene ID" value="Rmet_5905"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5905"/>
    <dbReference type="eggNOG" id="COG0583">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039613_16_2_4"/>
    <dbReference type="OMA" id="PSGYGQM"/>
    <dbReference type="OrthoDB" id="1661500at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005119">
        <property type="entry name" value="LysR_subst-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000847">
        <property type="entry name" value="Tscrpt_reg_HTH_LysR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00126">
        <property type="entry name" value="HTH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03466">
        <property type="entry name" value="LysR_substrate"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50931">
        <property type="entry name" value="HTH_LYSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH lysR-type" evidence="2">
        <location>
            <begin position="1"/>
            <end position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF12764.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="317" mass="34352" checksum="78001EC40D19C2F8" modified="2006-05-30" version="1">MDLNALRLFVDIVDAGNLSAAARKLKMTRANVSYHLKALEEELGVQLLRRTTRHVEPTPVGAGLYEHGKNILGEVAAANALISSMGKSLQGHVRLSVPTGLGHTMISPLLVRFKQRYPDITLDVVFDNRIHNLVSEDVEVALRIISTPPDSVVATEIGGVDWLLCAAPSYLAGRTPPAHLADLTTHAIVCASPIGQKLKVSGTRDGTADREQVVLEPTLSSENFAFLKEAVVGGLGIGIFPLYAVDKELASGALESLLGDYQISAFGSKLYMLTMPNRYQTMATRYLLTFLRTELQAIWPRIRAGGADSAYSENTQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="58" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LAX5</accession>
    <name>Q1LAX5_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_5842</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12701.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518050.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5842"/>
    <dbReference type="EnsemblBacteria" id="ABF12701">
        <property type="protein sequence ID" value="ABF12701"/>
        <property type="gene ID" value="Rmet_5842"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5842"/>
    <dbReference type="eggNOG" id="ENOG502Z9WS">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_055969_0_0_4"/>
    <dbReference type="OMA" id="WQETNCY"/>
    <dbReference type="OrthoDB" id="1097251at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014989">
        <property type="entry name" value="DUF1839"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08893">
        <property type="entry name" value="DUF1839"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF12701.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="350" mass="38501" checksum="F99503A753A857A1" modified="2006-05-30" version="1">MERGTETVLGTLAGCGGGADSAAAIAPANTRPGGLHGEDAIWQETNCYMDLWIELLYGWGLDPRAALAFTVTQDFEGDHFTFFKYPQADIEQLYGTVVQEMAVYDTLEAHVAEQVGRGHTVLVEVDSYYLPDTRATAYRRGHVKTTIAVDRIDTAAQRLSYYHSVGYYTAEGEDYRGLLRLDPALRDNGDVLFPYAECAKRVRTPLQGAALANAVAVQMRRHLGRRPAHNPVSRWREAFGDQVGNVLARGDAYFHHYGFNLPRQLGANFEMLGHCLTWLGEHGYRVPASAIRGAATIASECKVLQFRLARAVARGRPDLCAETLDTLEAAHSEVIDGLLQSFGSIDPAHS</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="108" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LB13</accession>
    <name>Q1LB13_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Transcriptional regulator, AraC family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_5804</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12663.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011520199.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5804"/>
    <dbReference type="EnsemblBacteria" id="ABF12663">
        <property type="protein sequence ID" value="ABF12663"/>
        <property type="gene ID" value="Rmet_5804"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5804"/>
    <dbReference type="eggNOG" id="COG2207">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_88_4_4"/>
    <dbReference type="OMA" id="HFSNRFR"/>
    <dbReference type="OrthoDB" id="1268974at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018060">
        <property type="entry name" value="HTH_AraC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018062">
        <property type="entry name" value="HTH_AraC-typ_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020449">
        <property type="entry name" value="Tscrpt_reg_HTH_AraC-type"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12833">
        <property type="entry name" value="HTH_18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00032">
        <property type="entry name" value="HTHARAC"/>
    </dbReference>
    <dbReference type="SMART" id="SM00342">
        <property type="entry name" value="HTH_ARAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00041">
        <property type="entry name" value="HTH_ARAC_FAMILY_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01124">
        <property type="entry name" value="HTH_ARAC_FAMILY_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH araC/xylS-type" evidence="2">
        <location>
            <begin position="193"/>
            <end position="291"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS01124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF12663.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="305" mass="33755" checksum="A078D07C4498CB8F" modified="2006-05-30" version="1">MSAPSTPLHDNAVFSALTQSRASLERACALGDGFGLALWRNRMDDTAYARPGHHTMSVYLEGGFSTYRRDAPDRKGAPDRLCLLPADHESRWHVGGTQRFMHLYFQPEHLAWHCVRVLDMEPRALQLQDRLFAEDDALVALTRQMVALDWEAPDMRMAANAMAHEAVTRMLLSHGTRQPSVDWRGGLSAAARRRVTEWVHAHPAANPTLGDLAALADLSEYHFARMFRISFGMPPHVWIMRARLSRACGLLGDRRLSLQQVADQAGFASASHFNNRFRAAYGVTPGQARASGMGALPSISASQGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="64" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LB41</accession>
    <name>Q1LB41_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_5776</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12635.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011520174.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5776"/>
    <dbReference type="EnsemblBacteria" id="ABF12635">
        <property type="protein sequence ID" value="ABF12635"/>
        <property type="gene ID" value="Rmet_5776"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5776"/>
    <dbReference type="eggNOG" id="COG5380">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_070057_0_0_4"/>
    <dbReference type="OMA" id="VDGTDPW"/>
    <dbReference type="OrthoDB" id="1366675at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="193"/>
            <end position="252"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF12635.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="341" mass="37365" checksum="8BBA8B9292EB18BA" modified="2006-05-30" version="1">MGISRWFRRRGTEASGGGARGGRDVEDTIERMTALNPRLRLATRYQQRLAPDTAAALEYVRGLVAQLPAVHEACAADWSTDACIHACFATADDVSRALNGAATLRKFFDNNLAADEAYAVLGMTMVERHTLGVATEGDTVRSDVPQTTFSFSDHQLTMCEPTEAALREEIVRRMLDQLAIQGMARIASRLTKRDALKQEIALLKTRQRLLESQGKGMGAVVGGAVEPAIGEVAKLDAEIARNDAELAKLSEATGTLDRQLDILIKVIGDVRQLLRVDYRHVRLSRMNVLLTNDDVGGDDVELSSATIPGDPPLVRAFMLVRVKRSAIVPVRNMLDEAERFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="65" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LB90</accession>
    <name>Q1LB90_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Putative capsular polysaccharide biosynthesis protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_5727</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12586.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011520127.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5727"/>
    <dbReference type="EnsemblBacteria" id="ABF12586">
        <property type="protein sequence ID" value="ABF12586"/>
        <property type="gene ID" value="Rmet_5727"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5727"/>
    <dbReference type="eggNOG" id="ENOG50333C9">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032721_0_0_4"/>
    <dbReference type="OMA" id="FKAHPWE"/>
    <dbReference type="OrthoDB" id="1757239at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000271">
        <property type="term" value="P:polysaccharide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015774">
        <property type="term" value="P:polysaccharide transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007833">
        <property type="entry name" value="Capsule_polysaccharide_synth"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05159">
        <property type="entry name" value="Capsule_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF12586.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="514" mass="57897" checksum="DD8534B61CE992C6" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="103" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LBG9</accession>
    <name>Q1LBG9_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Transcriptional regulator, AsnC family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Rmet_5648</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="6">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="6">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12507.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011520052.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5648"/>
    <dbReference type="EnsemblBacteria" id="ABF12507">
        <property type="protein sequence ID" value="ABF12507"/>
        <property type="gene ID" value="Rmet_5648"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5648"/>
    <dbReference type="eggNOG" id="COG1522">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_091233_5_3_4"/>
    <dbReference type="OMA" id="RIRAWMT"/>
    <dbReference type="OrthoDB" id="1844486at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000485">
        <property type="entry name" value="AsnC-type_HTH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011008">
        <property type="entry name" value="Dimeric_a/b-barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019888">
        <property type="entry name" value="Tscrpt_reg_AsnC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019887">
        <property type="entry name" value="Tscrpt_reg_AsnC/Lrp_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01037">
        <property type="entry name" value="AsnC_trans_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13404">
        <property type="entry name" value="HTH_AsnC-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00033">
        <property type="entry name" value="HTHASNC"/>
    </dbReference>
    <dbReference type="SMART" id="SM00344">
        <property type="entry name" value="HTH_ASNC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54909">
        <property type="entry name" value="SSF54909"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50956">
        <property type="entry name" value="HTH_ASNC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0614">Plasmid</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH asnC-type" evidence="4">
        <location>
            <begin position="1"/>
            <end position="62"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50956"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABF12507.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="140" mass="15779" checksum="64504418D23F231B" modified="2006-05-30" version="1">MDDTDRQLIALLRDDARTPVVTLARKLRVARATVQNRLAKLESEGTIVGYTIRLRPEAEAFRIRAIMSIEIEGNHSEDVRRELRGHPNVVALHTTNGRWDLMAELRADTLEAFDRILSAIRLIPGIANTETSILLSTLKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LBR1</accession>
    <name>Q1LBR1_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Alpha-methylacyl-CoA racemase</fullName>
            <ecNumber evidence="1">5.1.99.4</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_5556</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EC" id="5.1.99.4" evidence="1"/>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12415.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5556"/>
    <dbReference type="EnsemblBacteria" id="ABF12415">
        <property type="protein sequence ID" value="ABF12415"/>
        <property type="gene ID" value="Rmet_5556"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5556"/>
    <dbReference type="eggNOG" id="COG1804">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033975_5_0_4"/>
    <dbReference type="OMA" id="GYWKDER"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008111">
        <property type="term" value="F:alpha-methylacyl-CoA racemase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1540.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10540">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003673">
        <property type="entry name" value="CoA-Trfase_fam_III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044855">
        <property type="entry name" value="CoA-Trfase_III_dom3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023606">
        <property type="entry name" value="CoA-Trfase_III_dom_1_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02515">
        <property type="entry name" value="CoA_transf_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89796">
        <property type="entry name" value="SSF89796"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0413">Isomerase</keyword>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF12415.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="369" mass="39756" checksum="6A4C99F120E1C940" modified="2006-05-30" version="1">MLKPEHGRGPLNGVRVVEFAGLGPAPFACMMLADMGADVVRIDRPGTKARDNDLVGRGRRTVLLDLKAEGDIERARSLLAQADILVEGFRPGVMERLGLGPDTLLNINPRLVYGRMTGWGQDGPLSLAAGHDINYIALTGALAAIGDGDGLPVPPLNLVGDYGGGSLYLLVGILAALHNSRETGHGQVIDAAICDGAMSLMTFAQSQRLNGLFKEQRSSNMLDGGAPYYTTYRTKDDKVVCVGAIEPQFFALLCEKLGVKPELRDAQNDRKRWPILRAELEQIFKGQTQAEWTALLEGTDSCFSPVISLGEVSSHPHMAARGALTEVDGVACPAPAPRFSRTPTVISDLDRLLTTAEDISRQWESTNNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="55" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LBT1</accession>
    <name>Q1LBT1_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_5536</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12395.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519941.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5536"/>
    <dbReference type="EnsemblBacteria" id="ABF12395">
        <property type="protein sequence ID" value="ABF12395"/>
        <property type="gene ID" value="Rmet_5536"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5536"/>
    <dbReference type="eggNOG" id="ENOG5030717">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_083007_1_0_4"/>
    <dbReference type="OMA" id="CSMARGW"/>
    <dbReference type="OrthoDB" id="1739479at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021312">
        <property type="entry name" value="DUF2889"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11136">
        <property type="entry name" value="DUF2889"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF12395.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="192" mass="21987" checksum="EFCF8BD81D687A1A" modified="2006-05-30" version="1">MNDERVLLHTRQVQYRCYRRGDGLWEIEGEMRDFRSYDTVVAEKGNLPAGTSVHHMVIALVVDEELAVQNVVSRMEAVPFRVCREIENSLKTMVGVRMGYGWRHTINERLGGTESCTHLRELLINMATAAFQAIPTWQAQQRKQQGEDPLNGTRPHYLNQCKSWRIDGPVVREHLPQFYQPNAVAGAEPGKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LC08</accession>
    <name>Q1LC08_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transposase Tn7-like proteinA</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">tnsA</name>
        <name evidence="2" type="ordered locus">Rmet_5459</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12318.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5459"/>
    <dbReference type="EnsemblBacteria" id="ABF12318">
        <property type="protein sequence ID" value="ABF12318"/>
        <property type="gene ID" value="Rmet_5459"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5459"/>
    <dbReference type="eggNOG" id="ENOG502Z9E1">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_076083_0_1_4"/>
    <dbReference type="OMA" id="RAKNIEW"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1350.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011335">
        <property type="entry name" value="Restrct_endonuc-II-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014833">
        <property type="entry name" value="TnsA_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011856">
        <property type="entry name" value="tRNA_endonuc-like_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08722">
        <property type="entry name" value="Tn7_Tnp_TnsA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52980">
        <property type="entry name" value="SSF52980"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tn7_Tnp_TnsA_N" evidence="1">
        <location>
            <begin position="128"/>
            <end position="215"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF08722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF12318.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="317" mass="35998" checksum="5E3790709C9C2F7A" modified="2006-05-30" version="1">MHATKASPGFEQLSELDLTKKSQDLSAVAKRLLNPAVSGRAKGKEVIRTRKAGVWSPFEMNPQKLERFLREGRGMGSGNAYRPWIQVNDFSSLGRVHRTSSALTGYRTHHLLSDNEYYAYLEAWWDEEMLDIREQYPLLPVALTCGIAAELGFRHPYSTKYRLAIPLTTDLLLVRKRGLEAVAVVEDAHYEEQRTEEKLAIAEEFWRRRGVPTRVALSSTLKVQRSRNLHWIYQARRAPTERPYAPGANPLHDCLVGQVEGGPMSFWELAFRADLALCLPMGTSIAAIRWLLAERVLSTDIDDGDITKDCEIFVRSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="66" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LC61</accession>
    <name>Q1LC61_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_5406</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12265.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519813.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5406"/>
    <dbReference type="EnsemblBacteria" id="ABF12265">
        <property type="protein sequence ID" value="ABF12265"/>
        <property type="gene ID" value="Rmet_5406"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5406"/>
    <dbReference type="eggNOG" id="ENOG503337X">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_126556_0_0_4"/>
    <dbReference type="OMA" id="DYHPVAT"/>
    <dbReference type="OrthoDB" id="2032302at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="38"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF12265.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="175" mass="17378" checksum="A0AFA41B623CF734" modified="2006-05-30" version="1">MKHSLNQAIVALLGAVIVVASGWAPVAEARGGGGGGGFHGGGGGGGGGRGGGGAAMNAQANRPADARANNVRSTSVNSVNTNRNVNVNTNRNVNVNVENHGGCCGGGWDNDYHPVATAAAVTATVAVTSAVIGSVVRSVPPSCAPVNYGGMVYQQCGTTWYQPQGSQYVVVNAPY</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="106" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LCH2</accession>
    <name>Q1LCH2_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Transcriptional regulator, AraC family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Rmet_5295</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="7">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="7">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12154.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519700.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5295"/>
    <dbReference type="EnsemblBacteria" id="ABF12154">
        <property type="protein sequence ID" value="ABF12154"/>
        <property type="gene ID" value="Rmet_5295"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5295"/>
    <dbReference type="eggNOG" id="COG2207">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047522_1_2_4"/>
    <dbReference type="OMA" id="PDLMIPV"/>
    <dbReference type="OrthoDB" id="1622875at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032687">
        <property type="entry name" value="AraC-type_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018060">
        <property type="entry name" value="HTH_AraC"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12625">
        <property type="entry name" value="Arabinose_bd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12833">
        <property type="entry name" value="HTH_18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00342">
        <property type="entry name" value="HTH_ARAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01124">
        <property type="entry name" value="HTH_ARAC_FAMILY_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0614">Plasmid</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH araC/xylS-type" evidence="5">
        <location>
            <begin position="233"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="327"/>
            <end position="347"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS01124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABF12154.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="347" mass="38192" checksum="26CDA57A605F3465" modified="2006-05-30" version="1">MSLLVRAAALTNYSEVARAAGLDPVRMMMDAGISPSVLREPDLMIPVERYGRLLQTSATMSGNESFGLAMAESRLLSNLGAVGMLIRDQATLRDSLTLLMRYQPLLNGAQSLAVEDCGGRVIIREVLIAGNAHQPTRQRVELALGVMVRLIRQLIKADWQPQRVCLEHPAPHDLSAHHRFFGPHVEFDCDFNGIICAKADLDARNPWADPAMARYAQQLIDESAVMQQATMLEDVRRTILLLLPSGRCSAEQVADHLGVVCRTVQRRLAERGQSFSSMVNEIRTELATRHVIESDRPLTEVATLLGFSAPSGFSRWYHAQFGCSPKESRAAHGTTRRQTASAPASNA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LDG6</accession>
    <name>Q1LDG6_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Putative short-chain dehydrogenase/reductase (SDR)</fullName>
            <ecNumber evidence="1">1.1.1.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_4948</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EC" id="1.1.1.-" evidence="1"/>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11810.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519369.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4948"/>
    <dbReference type="EnsemblBacteria" id="ABF11810">
        <property type="protein sequence ID" value="ABF11810"/>
        <property type="gene ID" value="Rmet_4948"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4948"/>
    <dbReference type="eggNOG" id="COG1028">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010194_1_0_4"/>
    <dbReference type="OMA" id="CQVFGQY"/>
    <dbReference type="OrthoDB" id="1356861at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00061">
        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF11810.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="270" mass="28642" checksum="051BFC26D2E7756A" modified="2006-05-30" version="1">MEQIPDPQPASRSLKGRVAIVTGAGSRADGIGNGRAAAILLARDGARVALIDSVREWAEVTHRMIVEEGGEAEVIACDVSRDEDCRSAVARAVELWGRVDILVNNVGIGGPQGTAEDVDLEAWDQALRINVTSMMLMARHAIPHMRAQGKGAIVNVASVAGLIGGHPSLLYPTSKGAVVNMTRAMAAHHGEQGIRVNCVAPGMVYTPMVYAKGMSPEKREARRNRSFLRTEGSGWDVGQAIRYLVSDESGWVTGAVLPVDAGATAGRHSY</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="70" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LDM1</accession>
    <name>Q1LDM1_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_4893</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11755.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519315.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4893"/>
    <dbReference type="EnsemblBacteria" id="ABF11755">
        <property type="protein sequence ID" value="ABF11755"/>
        <property type="gene ID" value="Rmet_4893"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4893"/>
    <dbReference type="eggNOG" id="COG3558">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_112790_0_0_4"/>
    <dbReference type="OMA" id="VRFQYEC"/>
    <dbReference type="OrthoDB" id="1650155at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009783">
        <property type="entry name" value="DUF1348"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032710">
        <property type="entry name" value="NTF2-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31757">
        <property type="entry name" value="PTHR31757"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07080">
        <property type="entry name" value="DUF1348"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54427">
        <property type="entry name" value="SSF54427"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF11755.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="159" mass="18813" checksum="7F4A1301B46966FE" modified="2006-05-30" version="1">MSNVPEVRPPLPPFTRESAIAKVRAAEDGWNTRDPARVALAYTIDSAWRNRAEFVHGREQIQAFLARKWQKELDYRLIKELWAYEGNRIAVRFAYEWHDDSGNWFRSYGNENWEFDENGLMRQRHASINDAPIRESDRKYHWPLGRRPDDHPGLSDLGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="103" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LDN2</accession>
    <name>Q1LDN2_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Benzoate 1,2-dioxygenase, alpha subunit</fullName>
            <ecNumber evidence="10">1.14.12.10</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">benA</name>
        <name evidence="10" type="ordered locus">Rmet_4882</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="11">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="11">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the bacterial ring-hydroxylating dioxygenase alpha subunit family.</text>
    </comment>
    <dbReference type="EC" id="1.14.12.10" evidence="10"/>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11744.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519303.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4882"/>
    <dbReference type="EnsemblBacteria" id="ABF11744">
        <property type="protein sequence ID" value="ABF11744"/>
        <property type="gene ID" value="Rmet_4882"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4882"/>
    <dbReference type="eggNOG" id="COG4638">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026244_4_1_4"/>
    <dbReference type="OMA" id="TRNLCLY"/>
    <dbReference type="OrthoDB" id="275867at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018623">
        <property type="term" value="F:benzoate 1,2-dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019439">
        <property type="term" value="P:aromatic compound catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.102.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017639">
        <property type="entry name" value="Benzo_1-2-diOase_lsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017941">
        <property type="entry name" value="Rieske_2Fe-2S"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036922">
        <property type="entry name" value="Rieske_2Fe-2S_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015881">
        <property type="entry name" value="Ring-hydroxy_dOase_2Fe2S_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015879">
        <property type="entry name" value="Ring_hydroxy_dOase_asu_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001663">
        <property type="entry name" value="Rng_hydr_dOase-A"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43756">
        <property type="entry name" value="PTHR43756"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00355">
        <property type="entry name" value="Rieske"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00848">
        <property type="entry name" value="Ring_hydroxyl_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00090">
        <property type="entry name" value="RNGDIOXGNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50022">
        <property type="entry name" value="SSF50022"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03229">
        <property type="entry name" value="benzo_1_2_benA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51296">
        <property type="entry name" value="RIESKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00570">
        <property type="entry name" value="RING_HYDROXYL_ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0001">2Fe-2S</keyword>
    <keyword evidence="4" id="KW-0058">Aromatic hydrocarbons catabolism</keyword>
    <keyword evidence="10" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="6" id="KW-0408">Iron</keyword>
    <keyword evidence="7" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0520">NAD</keyword>
    <keyword evidence="5 10" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-0614">Plasmid</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rieske" evidence="9">
        <location>
            <begin position="64"/>
            <end position="161"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008751"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022797"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ABF11744.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="464" mass="53004" checksum="945B8504EC2CF7C8" modified="2006-05-30" version="1">MIPIHLDTGAGPARPQHQPNRLENLDQFLVEDKTTGDYRLHRSAFTDEALFELEMQHIFEGNWIYLAHESQIPNNNDYYTTHIGRQPIVIARNRQGELNAFINACSHRGAMLCRHKRGNKATYTCPFHGWTFNNSGKLLKVKDPEQAGYPECFNKEGSHDLKKLARFSNYRGFLFGSLNADVPPIEQFLGDAARIIDMIVDQSPDGLEVLRGSSTYTFAGNWKLQAENGADGYHVSAVHWNYAATTSHRKQQNEREDKIRAMDAGKWGQQGGGFYAFDHGHMLLWSRWANPEDRPNFARRDEFAQRCGEERADWMIQNSRNLCLYPNVYLMDQFGSQIRLLRPLSVDRTEVTIYCIAPKGESDEARARRIRQYEDFFNVSGMATPDDLEEFRACQQGYAGQALAWNDMCRGATHWIDGPDDAAKHIGLNPVMSGLRTEDEGLYTVQHRYWLETMKRAVAQGDAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="100" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LDR7</accession>
    <name>Q1LDR7_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Putative transporter, YidE/YbjL duplication</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">Rmet_4847</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="11">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="11">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the AAE transporter (TC 2.A.81) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11709.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519272.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4847"/>
    <dbReference type="EnsemblBacteria" id="ABF11709">
        <property type="protein sequence ID" value="ABF11709"/>
        <property type="gene ID" value="Rmet_4847"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4847"/>
    <dbReference type="eggNOG" id="COG2985">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035023_2_2_4"/>
    <dbReference type="OMA" id="GPQFFAN"/>
    <dbReference type="OrthoDB" id="1458740at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006813">
        <property type="term" value="P:potassium ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022457">
        <property type="entry name" value="Asp_Ala_antiprt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036721">
        <property type="entry name" value="RCK_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006512">
        <property type="entry name" value="YidE_YbjL"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06826">
        <property type="entry name" value="Asp-Al_Ex"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF116726">
        <property type="entry name" value="SSF116726"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03802">
        <property type="entry name" value="Asp_Ala_antiprt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01625">
        <property type="entry name" value="YidE_YbjL_dupl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0614">Plasmid</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="6"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="34"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="63"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="93"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="164"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="387"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="410"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="451"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="478"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="508"/>
            <end position="529"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="541"/>
            <end position="562"/>
        </location>
    </feature>
    <feature type="domain" description="Asp-Al_Ex" evidence="9">
        <location>
            <begin position="15"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="domain" description="Asp-Al_Ex" evidence="9">
        <location>
            <begin position="389"/>
            <end position="558"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009854"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF06826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ABF11709.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="567" mass="59437" checksum="6BFE69A3A7A8A6EA" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="68" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LEX7</accession>
    <name>Q1LEX7_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_4434</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11299.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4434"/>
    <dbReference type="EnsemblBacteria" id="ABF11299">
        <property type="protein sequence ID" value="ABF11299"/>
        <property type="gene ID" value="Rmet_4434"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4434"/>
    <dbReference type="eggNOG" id="COG3797">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_106303_2_0_4"/>
    <dbReference type="OMA" id="CFEAAGF"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012545">
        <property type="entry name" value="DUF1697"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36439">
        <property type="entry name" value="PTHR36439"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08002">
        <property type="entry name" value="DUF1697"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF008502">
        <property type="entry name" value="UCP008502"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF11299.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="159" mass="17360" checksum="C8A5D52FC0855FD9" modified="2006-05-30" version="1">MNARMPELKHCFEAAGFSEVRTLLSSGNVVFDARSASTTALERRAEKAMHAELGHSFGTFIRPAGYLRDLIEADPFAEFDLAPGAKRVVTFLRSPVAPEITLPIERDGASIIKASAAEVFSAYLPDPKKGPVFMTLLERSFGKDITTRTLDTVIKCARA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-02-10" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LF90</accession>
    <name>Q1LF90_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Transcriptional regulator, AraC family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_4321</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11186.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4321"/>
    <dbReference type="EnsemblBacteria" id="ABF11186">
        <property type="protein sequence ID" value="ABF11186"/>
        <property type="gene ID" value="Rmet_4321"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4321"/>
    <dbReference type="eggNOG" id="COG2207">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_049704_5_0_4"/>
    <dbReference type="OrthoDB" id="1785082at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035418">
        <property type="entry name" value="AraC-bd_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018060">
        <property type="entry name" value="HTH_AraC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001647">
        <property type="entry name" value="HTH_TetR"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14525">
        <property type="entry name" value="AraC_binding_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00440">
        <property type="entry name" value="TetR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01124">
        <property type="entry name" value="HTH_ARAC_FAMILY_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH araC/xylS-type" evidence="2">
        <location>
            <begin position="213"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="301"/>
            <end position="323"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS01124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF11186.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="323" mass="34523" checksum="D13B9AF9A0833715" modified="2006-05-30" version="1">MSAIVTWSTNAVPRTQRLDYFAAALSSALVPMQVEATHTGALASHMSMLEIDDVDVLSQCGSAHRSLRGRAELARSGAHTYHLIVNRRAAWYVEHAGATRVRAGQAFLIDSARANLIEMPSAYEVTHVKMPEAWLRRWVREPSRMVGRPLGGEAGQMDHALAAFVAGMTPHALRDGPLPASMMIDHLGAMLAMASGTTGRPIAASSAALALYNKVFACIANRSAEPLLSAGDIANSLAIAESELHSVLASFGDTFASLLVTHRLANARRMLASRAFEAFTVPEIALRAGFSRGADLTNLLEPGDTSGSDACPARDRPKRLRSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="97" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LFC2</accession>
    <name>Q1LFC2_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Short chain dehydrogenase</fullName>
            <ecNumber evidence="2">1.1.1.62</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_4288</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.62" evidence="2"/>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11154.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518779.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4288"/>
    <dbReference type="EnsemblBacteria" id="ABF11154">
        <property type="protein sequence ID" value="ABF11154"/>
        <property type="gene ID" value="Rmet_4288"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4288"/>
    <dbReference type="eggNOG" id="COG4221">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010194_2_9_4"/>
    <dbReference type="OMA" id="QDPLPAC"/>
    <dbReference type="OrthoDB" id="1582456at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004303">
        <property type="term" value="F:estradiol 17-beta-dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU000363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF11154.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="287" mass="30627" checksum="F8631DFE2CCE32D2" modified="2006-05-30" version="1">MHSKVALVTGASSGIGEATALKLLAQGYTVYAAARRVERMRSQADAGVHVLAMDVTDDAVMRAGIEEIIAKSGRLDVLVNNAGYGAYGAIEDVPLDEARAQFDVNVFGAVRLIQLALPHMRAQRSGTIVNITSMGGKIHTPMGAWYHGTKFALEAISDCLRMEVEPFGIDVVVIEPGGIKTEWAGIAAENLLKVSGTGSYAPQASAMAKLMINEANGNRQSPPQLIAETIAKAVNAARPKTRYAVGFGARPMILMRRLLSDRAFDSLMRMAARMPRSAQRRSCGAAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LFJ4</accession>
    <name>Q1LFJ4_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Response regulator, signal receiver domain protein (CheY-like)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_4215</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11082.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4215"/>
    <dbReference type="EnsemblBacteria" id="ABF11082">
        <property type="protein sequence ID" value="ABF11082"/>
        <property type="gene ID" value="Rmet_4215"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4215"/>
    <dbReference type="eggNOG" id="COG0784">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_69_12_4"/>
    <dbReference type="OrthoDB" id="1755994at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Response regulatory" evidence="2">
        <location>
            <begin position="16"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="1">
        <location>
            <position position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF11082.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="150" mass="15991" checksum="4A3625688170FDEF" modified="2006-05-30" version="1">MPRTVSKPANNLPLPRVIVAEDHPVCLLVLEDQLKGIGGCEVVLCENGADALRALESGPAALLLTDLSLPGIEGLALARRIRTGKAGQVAPDLPIVAITATAGPEERLACLEAGIGMVLTKPVSYETLQSVLGRYLGKDDQYDQYEQYDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="50" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LFK0</accession>
    <name>Q1LFK0_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_4209</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11076.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518699.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF11076">
        <property type="protein sequence ID" value="ABF11076"/>
        <property type="gene ID" value="Rmet_4209"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4209"/>
    <dbReference type="HOGENOM" id="CLU_1990832_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF11076.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="125" mass="13490" checksum="147272699A45FEFA" modified="2006-05-30" version="1">MDDTIEFSTSRPASQTGTEGPCPHLLALRGCMRHDGPPLTPGQIDLLIEASVMEEFMSSMGWAPTTVVNAGDWPALEDMPYAGGAANPLFGCLAQTYREQGVEFYVPNTDFFRPLAAGQPGRRQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-10-07" version="51" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LFK1</accession>
    <name>Q1LFK1_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_4208</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11075.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF11075">
        <property type="protein sequence ID" value="ABF11075"/>
        <property type="gene ID" value="Rmet_4208"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4208"/>
    <dbReference type="eggNOG" id="COG5611">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2452428_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029060">
        <property type="entry name" value="PIN-like_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88723">
        <property type="entry name" value="SSF88723"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF11075.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="89" mass="9955" checksum="E015347A06DFBC73" modified="2006-05-30" version="1">MIGLDTCVLARLILDDDAVQSPIAAGLIASLTCQRPGYVSTAVILELAWVMQRRRSRPEIIRAIYRLLRSRALRVEGGFKSKCNKNSMH</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LFP1</accession>
    <name>Q1LFP1_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Putative hopanoid biosynthesis associated radical SAM protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">hpnH</name>
        <name evidence="7" type="ordered locus">Rmet_4168</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="8">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="8">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11035.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518656.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4168"/>
    <dbReference type="EnsemblBacteria" id="ABF11035">
        <property type="protein sequence ID" value="ABF11035"/>
        <property type="gene ID" value="Rmet_4168"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4168"/>
    <dbReference type="eggNOG" id="COG0535">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_052502_0_0_4"/>
    <dbReference type="OMA" id="FNTDTPQ"/>
    <dbReference type="OrthoDB" id="1172757at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022563">
        <property type="entry name" value="DUF3463"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017833">
        <property type="entry name" value="Hopanoid_synth-assoc_rSAM_HpnH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11946">
        <property type="entry name" value="DUF3463"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00397">
        <property type="entry name" value="adenosyl-hopene_transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03470">
        <property type="entry name" value="HpnH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0408">Iron</keyword>
    <keyword evidence="5" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0614">Plasmid</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <feature type="domain" description="Radical SAM core" evidence="6">
        <location>
            <begin position="20"/>
            <end position="228"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABF11035.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="388" mass="43476" checksum="0179EB6BBB5CB945" modified="2006-05-30" version="1">MAIPFLQVARVGAYIARKHLSGQKRYPLALMLEPLFRCNLACSGCGKIDYPDPILNQRLSVQECLEAVDECGAPVVSIAGGEPLLHKDMPEIVRGIIARKRFVYLCTNALLMEKKIDQYEPSPYFVWSIHLDGDREMHDHSVNQEGVYDRCVEAIRMAKERGFRVNINCTLFNDAKPDRVAKFFDSVKAMGVDGITVSPGYAYERAPDQQHFLHRGKTKQLFRDILSRGNAGKNWAFSQSTMFLDFLAGNQTYKCTPWGNPARTVFGWQRPCYLVGEGYVKTFKELMEDTNWDAYGVGNYEKCANCMVHSGFEATAVADTFAHPIKALGVSLRGVKTDGPMAPDIPLDKQRPAEYVFSRHVEIKLAEIGNLKKAQGSRAEAVQAAQVH</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="109" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LFQ8</accession>
    <name>Q1LFQ8_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Cold-shock DNA-binding domain protein, anti-terminator of transcription</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Rmet_4151</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="5">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="5">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11018.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008650922.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4151"/>
    <dbReference type="EnsemblBacteria" id="ABF11018">
        <property type="protein sequence ID" value="ABF11018"/>
        <property type="gene ID" value="Rmet_4151"/>
    </dbReference>
    <dbReference type="GeneID" id="60823265"/>
    <dbReference type="KEGG" id="rme:Rmet_4151"/>
    <dbReference type="eggNOG" id="COG1278">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_117621_0_3_4"/>
    <dbReference type="OMA" id="ICEMAKI"/>
    <dbReference type="OrthoDB" id="2034748at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04458">
        <property type="entry name" value="CSP_CDS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012156">
        <property type="entry name" value="Cold_shock_CspA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011129">
        <property type="entry name" value="CSD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019844">
        <property type="entry name" value="CSD_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002059">
        <property type="entry name" value="CSP_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00313">
        <property type="entry name" value="CSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002599">
        <property type="entry name" value="Cold_shock_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00050">
        <property type="entry name" value="COLDSHOCK"/>
    </dbReference>
    <dbReference type="SMART" id="SM00357">
        <property type="entry name" value="CSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00352">
        <property type="entry name" value="CSD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51857">
        <property type="entry name" value="CSD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0614">Plasmid</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CSD" evidence="3">
        <location>
            <begin position="1"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU000408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF11018.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="67" mass="7268" checksum="CB2839D6B3C26AC8" modified="2006-05-30" version="1">METGTVKWFNDAKGFGFITPDAGGNDLFAHFSAIEGNGFKSLKEGQKVRYVKAMGQKGEQATKIQAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LG43</accession>
    <name>Q1LG43_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">3-carboxy-cis,cis-muconate cycloisomerase</fullName>
            <ecNumber evidence="2">5.5.1.2</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">pcaB</name>
        <name evidence="2" type="ordered locus">Rmet_4015</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EC" id="5.5.1.2" evidence="2"/>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10883.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4015"/>
    <dbReference type="EnsemblBacteria" id="ABF10883">
        <property type="protein sequence ID" value="ABF10883"/>
        <property type="gene ID" value="Rmet_4015"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4015"/>
    <dbReference type="eggNOG" id="COG0015">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030949_3_3_4"/>
    <dbReference type="OMA" id="HRATPMI"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047472">
        <property type="term" value="F:3-carboxy-cis,cis-muconate cycloisomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016840">
        <property type="term" value="F:carbon-nitrogen lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019619">
        <property type="term" value="P:3,4-dihydroxybenzoate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.275.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019468">
        <property type="entry name" value="AdenyloSucc_lyase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024083">
        <property type="entry name" value="Fumarase/histidase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020557">
        <property type="entry name" value="Fumarate_lyase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000362">
        <property type="entry name" value="Fumarate_lyase_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022761">
        <property type="entry name" value="Fumarate_lyase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008948">
        <property type="entry name" value="L-Aspartase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012789">
        <property type="entry name" value="Protocat_PcaB"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10397">
        <property type="entry name" value="ADSL_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00206">
        <property type="entry name" value="Lyase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00149">
        <property type="entry name" value="FUMRATELYASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00998">
        <property type="entry name" value="ADSL_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48557">
        <property type="entry name" value="SSF48557"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02426">
        <property type="entry name" value="protocat_pcaB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00163">
        <property type="entry name" value="FUMARATE_LYASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0413">Isomerase</keyword>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ADSL_C" evidence="1">
        <location>
            <begin position="395"/>
            <end position="478"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00998"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF10883.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="490" mass="51781" checksum="725E17EAF271DEA2" modified="2006-05-30" version="1">MHCKQGAGFPPCPALHDNRRITTGLPPPTMPTLSRLTDPMFGSPAVLEVFSDTGTVRRMLDAEAALARAEAQCGVIPATAAAVITEVCGRVDVIDLEALAQAAVSAGNLAIPFVKQLTAAVTQRDADAGRYVHWGATSQDIIDTALVLQLRDALREIVVDLLRAGDACATLAATHRATPMVARTWLQHALPTTFGCKAGGWLDALRRDLTRLDHVGAQVRVIQFGGAAGTLASLGDDAPAVAARFAAELQLDVPALPWHAHRDRLVEVATALGMLTGTLGKMARDVSLMMQTEVGEVAEPSGPGRGGSSTMPHKRNPVGCAAVLTAATRVPPLVATMLAGMTQEHERALGGWQAEWDTLPQIVTLAAGALRQMADVASGLEVDEARMRANLDITHGLILGEATMLELGRRIGRLQAHHLVEQASRRAAAEDTTLRIALARTLAEDANHAGLLDDAALDRLSDPAQYAGQSERFTDAAVAAWQALRANPQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="95" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LG68</accession>
    <name>Q1LG68_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">GTP cyclohydrolase I</fullName>
            <ecNumber evidence="3">3.5.4.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">folE</name>
        <name evidence="8" type="ordered locus">Rmet_3990</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="9">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="9">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>GTP + H2O = 7,8-dihydroneopterin 3'-triphosphate + formate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17473"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15740"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:58462"/>
            <dbReference type="EC" id="3.5.4.16"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Cofactor biosynthesis; 7,8-dihydroneopterin triphosphate biosynthesis; 7,8-dihydroneopterin triphosphate from GTP: step 1/1.</text>
    </comment>
    <dbReference type="EC" id="3.5.4.16" evidence="3"/>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10858.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518498.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3990"/>
    <dbReference type="EnsemblBacteria" id="ABF10858">
        <property type="protein sequence ID" value="ABF10858"/>
        <property type="gene ID" value="Rmet_3990"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3990"/>
    <dbReference type="eggNOG" id="COG0302">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_049768_3_0_4"/>
    <dbReference type="OMA" id="VACVIDA"/>
    <dbReference type="OrthoDB" id="1632367at2"/>
    <dbReference type="UniPathway" id="UPA00848">
        <property type="reaction ID" value="UER00151"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003934">
        <property type="term" value="F:GTP cyclohydrolase I activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035998">
        <property type="term" value="P:7,8-dihydroneopterin 3'-triphosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006730">
        <property type="term" value="P:one-carbon metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046654">
        <property type="term" value="P:tetrahydrofolate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.286.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1130.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043133">
        <property type="entry name" value="GTP-CH-I_C/QueF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043134">
        <property type="entry name" value="GTP-CH-I_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001474">
        <property type="entry name" value="GTP_CycHdrlase_I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020602">
        <property type="entry name" value="GTP_CycHdrlase_I_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11109">
        <property type="entry name" value="PTHR11109"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01227">
        <property type="entry name" value="GTP_cyclohydroI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0554">One-carbon metabolism</keyword>
    <keyword evidence="8" id="KW-0614">Plasmid</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GTP_cyclohydroI" evidence="7">
        <location>
            <begin position="57"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012715"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01227"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ABF10858.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="241" mass="27639" checksum="3F2448A15A1FD786" modified="2006-05-30" version="1">MSHKDQSDRPEHTPTASPVSNRIRARIEAAGERFHANDNISRHIEHGELEALQAEVEARMEDVLRTLVIDVDQDHNTRETARRVAKMYLKEIFAGRYAKAPDVTEFPNVEQLNELMIVGPIRVRSACSHHLCPIIGSLWVGVMPNRHSNLIGLSKYARLAEWIMCRPQIQEEAVAQVADLLQEKMNPDGLAIVMEAEHFCMHWRGVRDTDAKMTNSVMRGSFLKDDKLRREFLTLLNLNRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="51" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LG71</accession>
    <name>Q1LG71_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_3987</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10855.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518495.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF10855">
        <property type="protein sequence ID" value="ABF10855"/>
        <property type="gene ID" value="Rmet_3987"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3987"/>
    <dbReference type="HOGENOM" id="CLU_893924_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF10855.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="311" mass="34075" checksum="420F291C17757172" modified="2006-05-30" version="1">MQTKSSTLRETEQQRQTILQTARAQAEALNGMYQNTLSAFLVAPNAMPLPRAARAMFWLGRLRQWRAIDLSGDEACSPPNVTSLQLGVIESIAGTHALALHKEKWSGPHSRQAYERDMALQVAMLLALEWRELAAFALQAWFGTNSELASLHPVSGMTGLIIGVAGQALGVGVPQVTFQKGDAVLSKIVNHWQDSDIAFAPLARELADRHVNQCRLDTDHSLFDFDHPVEQAIPVELLMLLRLRDGDKVPAWLARHGAFSHPAAVMLKATTPSQSQLCHSFITSATTLLPQYGDLSAALDRQISASRKLSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="66" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LG94</accession>
    <name>Q1LG94_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_3964</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10832.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518471.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3964"/>
    <dbReference type="EnsemblBacteria" id="ABF10832">
        <property type="protein sequence ID" value="ABF10832"/>
        <property type="gene ID" value="Rmet_3964"/>
    </dbReference>
    <dbReference type="GeneID" id="60823445"/>
    <dbReference type="KEGG" id="rme:Rmet_3964"/>
    <dbReference type="eggNOG" id="COG3333">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022936_2_0_4"/>
    <dbReference type="OMA" id="HPDVFWS"/>
    <dbReference type="OrthoDB" id="735109at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002823">
        <property type="entry name" value="DUF112_TM"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01970">
        <property type="entry name" value="TctA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="45"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="108"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="144"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="169"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="206"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="257"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="323"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="353"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="386"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="469"/>
            <end position="489"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF10832.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="500" mass="52617" checksum="BF99B8FB103B79CF" modified="2006-05-30" version="1">MELFDHLALGFSTALSLQNLAYAFLGCVLGTLIGVLPGLGPLATIAMLLPVTYSLPPVAALIMLAGIYYGAQYGGSTTAILVNLPGESSSVVTTIDGYQMARRGRAGVALATAGLGSFFAGCVATLILAAFATPLSELAFKFGPAEYFSLMVLGLIGAVVLASGSLPKAVAMIVLGLLMGLIGTDVNSGAARFSFDVPELTDGIDFVALAMGMFGFAEIIANLEQKEARETFTNKVTNLWPTKEDFRRMIPAVLRGTALGSALGILPGGGAALASFAAYSLEKKTSKYAHEFGKGAIEGVAGPESANNAAAQTSFIPLLTLGIPPNAVMALMVGAMTIHNIQPGPQVMSSNPALFWGLIASMWIGNLMLIILNLPMIGVWVKLLTVPYRFLYPAILVFCCIGVYSVNNQTFDVFMAAAFGVIGYLFLKLKCEPAPLLLGFVLGPMMEENFRRSLLLSRGDFTVFVTRPLSMGLLIAAAALVAVVALPSIKAKREEAFQEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="110" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LGH6</accession>
    <name>Q1LGH6_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Histidine kinase</fullName>
            <ecNumber evidence="2">2.7.13.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">envZ</name>
        <name evidence="9" type="ordered locus">Rmet_3882</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="10">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="10">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.</text>
            <dbReference type="EC" id="2.7.13.3"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.7.13.3" evidence="2"/>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10750.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518397.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3882"/>
    <dbReference type="EnsemblBacteria" id="ABF10750">
        <property type="protein sequence ID" value="ABF10750"/>
        <property type="gene ID" value="Rmet_3882"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3882"/>
    <dbReference type="eggNOG" id="COG2205">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG3850">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_89_27_4"/>
    <dbReference type="OMA" id="QFMDYAG"/>
    <dbReference type="OrthoDB" id="1031685at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00082">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003660">
        <property type="entry name" value="HAMP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003661">
        <property type="entry name" value="HisK_dim/P"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036097">
        <property type="entry name" value="HisK_dim/P_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004358">
        <property type="entry name" value="Sig_transdc_His_kin-like_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00672">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00344">
        <property type="entry name" value="BCTRLSENSOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00304">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00388">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47384">
        <property type="entry name" value="SSF47384"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="SSF55874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50885">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 9" id="KW-0418">Kinase</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9" id="KW-0614">Plasmid</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="14"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="154"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="domain" description="HAMP" evidence="8">
        <location>
            <begin position="177"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="7">
        <location>
            <begin position="237"/>
            <end position="437"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ABF10750.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="437" mass="47132" checksum="DB7815CBACF746A3" modified="2006-05-30" version="1">MRIGLPRSLLARNIALLVALVAFTQVCSLMVLLHFAQRPRVERAATVFAHYVSMLDGVMAAMPAEEARVAVARLGGQTDAPAVDDVMEARALHPFRTWQRDVFMDELHKALPPDMPVQWQDAGDNERLWIRIRAAGMPTWVALPMTADAQASGITAAIALSAALGLLAALTGYLIQRHINRPLQDLARAALHVSAGETPAPLPTDGPTEISQVSSAFNRMTEALQQTEKTRALMLAGISHDIRTPLTKLRLAMAMALPRGTDDAFVGSVEGYLDRIDTILQQFMDYAGSGEREAPQPGDLNALVRQLASDFAGLGHEFELDLGDLPPVSYRPTSMLRLLMNLMQNAINYGRTGLMVRTWKEGGAAHVAVCDRGIGISVAELEQLRAPFSRGDNARGHSGGTGLGLAIVERIARLHGGSLAFRPREGGGLEVVVSLPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LGS0</accession>
    <name>Q1LGS0_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Putative outer membrane porin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">Rmet_3786</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="10">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="10">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10656.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518302.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3786"/>
    <dbReference type="EnsemblBacteria" id="ABF10656">
        <property type="protein sequence ID" value="ABF10656"/>
        <property type="gene ID" value="Rmet_3786"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3786"/>
    <dbReference type="eggNOG" id="COG3203">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038238_2_0_4"/>
    <dbReference type="OMA" id="FSAMYSF"/>
    <dbReference type="OrthoDB" id="1064531at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009279">
        <property type="term" value="C:cell outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046930">
        <property type="term" value="C:pore complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015288">
        <property type="term" value="F:porin activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034220">
        <property type="term" value="P:ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00342">
        <property type="entry name" value="gram_neg_porins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.160.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033900">
        <property type="entry name" value="Gram_neg_porin_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023614">
        <property type="entry name" value="Porin_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001702">
        <property type="entry name" value="Porin_Gram-ve"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002299">
        <property type="entry name" value="Porin_Neis"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13609">
        <property type="entry name" value="Porin_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00182">
        <property type="entry name" value="ECOLNEIPORIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00184">
        <property type="entry name" value="NEISSPPORIN"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0406">Ion transport</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0614">Plasmid</keyword>
    <keyword evidence="5" id="KW-0626">Porin</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1134">Transmembrane beta strand</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193445" evidence="7">
        <location>
            <begin position="21"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="domain" description="Porin_4" evidence="8">
        <location>
            <begin position="7"/>
            <end position="350"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF13609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ABF10656.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="391" mass="41183" checksum="FDF53935F516B804" modified="2006-05-30" version="1">MKLNRFAAVALAACSGSALAQSSVTLYGVVDANIEYVNHVGAVPTAANGFNPGPGHDVVRMNSGGLSGSRWGIRGVEDLGAGTQAVFVLENGFSGDTGTMQQSSRLFGRQAFVGIKNATYGQISLGRQYTSLFDSIVNFVPAYFATQYEPITLFAGANFREDNTIKYTLNAGQFTGVAHYSFGTGLALPQTVQSGLAIGGNGEVPGQARRDSAYGASLSYAGGSLWAAIAYDQFNPTIGTTTSASSGSFKKATAALSYSLPWGRIMGGYRWGQNKTPAGATIGRDDFFWVGGIYQVGPALSLTLEYDYDKVRNVFGNTSVANPWQVNLIADYSLSKRTDVYLSAAYAKNAGLMLDSALIAYANSLSLGNSYVLGNGNSSMLGVSVGVRHKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LHI8</accession>
    <name>Q1LHI8_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">ABC-type transporter, permease component</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">livM5</name>
        <name evidence="7" type="ordered locus">Rmet_3516</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10388.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517939.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3516"/>
    <dbReference type="EnsemblBacteria" id="ABF10388">
        <property type="protein sequence ID" value="ABF10388"/>
        <property type="gene ID" value="Rmet_3516"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3516"/>
    <dbReference type="eggNOG" id="COG4177">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031365_0_1_4"/>
    <dbReference type="OMA" id="KMQALVL"/>
    <dbReference type="OrthoDB" id="1456257at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015658">
        <property type="term" value="F:branched-chain amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06581">
        <property type="entry name" value="TM_PBP1_LivM_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001851">
        <property type="entry name" value="ABC_transp_permease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043428">
        <property type="entry name" value="LivM-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30482">
        <property type="entry name" value="PTHR30482"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02653">
        <property type="entry name" value="BPD_transp_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="27"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="55"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="100"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="128"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="174"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="224"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="259"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="313"/>
            <end position="332"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABF10388.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="344" mass="36022" checksum="37415745458C937F" modified="2006-05-30" version="1">MSGPTGQTTAAVEAKGFKEGTGVQKKVLYGLLLLGLIAAPLAGAYPVFVLKVLCFALFACAFNLLIGFTGLLSFGHAAFFGGAGYAAGHAMKVWGVTPELGLIFGTLSGAAIGYVVGSLAIRRQGIYFSMITLALAQMLFFFCLQVPLTGGEDGLQGIPRGKLLGVLPLSDDLTLYYVALVIIVAAFALIVRTVHSPFGQILKAIRENEPRAISLGYDVDRFKLLAFVLSAALSGLAGSIKALVLGFETLTDVHWSMSGSVILMTLVGGLGTLSGPIVGAFVVVALENKLGDIGTFLASVTGLDWFNSLGESVTMVTGVIFVICVLTFRRGIMGELIALTSRKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="96" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LHU2</accession>
    <name>Q1LHU2_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Acyl-CoA dehydrogenase</fullName>
            <ecNumber evidence="8">1.3.8.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Rmet_3412</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the acyl-CoA dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.3.8.-" evidence="8"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10284.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517848.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3412"/>
    <dbReference type="EnsemblBacteria" id="ABF10284">
        <property type="protein sequence ID" value="ABF10284"/>
        <property type="gene ID" value="Rmet_3412"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3412"/>
    <dbReference type="eggNOG" id="COG1960">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019128_0_0_4"/>
    <dbReference type="OMA" id="RAWHAEL"/>
    <dbReference type="OrthoDB" id="760677at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016627">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-CH group of donors"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.540.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.110.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006091">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046373">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036250">
        <property type="entry name" value="AcylCo_DH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009075">
        <property type="entry name" value="AcylCo_DH/oxidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013786">
        <property type="entry name" value="AcylCoA_DH/ox_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037069">
        <property type="entry name" value="AcylCoA_DH/ox_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009100">
        <property type="entry name" value="AcylCoA_DH/oxidase_NM_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00441">
        <property type="entry name" value="Acyl-CoA_dh_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02770">
        <property type="entry name" value="Acyl-CoA_dh_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02771">
        <property type="entry name" value="Acyl-CoA_dh_N"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47203">
        <property type="entry name" value="SSF47203"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56645">
        <property type="entry name" value="SSF56645"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0274">FAD</keyword>
    <keyword evidence="3" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Acyl-CoA_dh_N" evidence="7">
        <location>
            <begin position="14"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA_dh_M" evidence="6">
        <location>
            <begin position="133"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA_dh_1" evidence="5">
        <location>
            <begin position="239"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA_dh_N" evidence="7">
        <location>
            <begin position="426"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA_dh_1" evidence="5">
        <location>
            <begin position="654"/>
            <end position="788"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF02770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF02771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ABF10284.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="800" mass="86641" checksum="5ADDF7EC67975024" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-12-02" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LHV3</accession>
    <name>Q1LHV3_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Extradiol ring-cleavage dioxygenase, class III enzyme, subunit B (Catalytic subunit)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Rmet_3401</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the DODA-type extradiol aromatic ring-opening dioxygenase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10273.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3401"/>
    <dbReference type="EnsemblBacteria" id="ABF10273">
        <property type="protein sequence ID" value="ABF10273"/>
        <property type="gene ID" value="Rmet_3401"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3401"/>
    <dbReference type="eggNOG" id="COG3384">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046582_2_1_4"/>
    <dbReference type="OMA" id="RTEHFAP"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051213">
        <property type="term" value="F:dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008198">
        <property type="term" value="F:ferrous iron binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016701">
        <property type="term" value="F:oxidoreductase activity, acting on single donors with incorporation of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006725">
        <property type="term" value="P:cellular aromatic compound metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07363">
        <property type="entry name" value="45_DOPA_Dioxygenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014436">
        <property type="entry name" value="Extradiol_dOase_DODA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004183">
        <property type="entry name" value="Xdiol_dOase_suB"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02900">
        <property type="entry name" value="LigB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006157">
        <property type="entry name" value="Doxgns_DODA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="LigB" evidence="5">
        <location>
            <begin position="7"/>
            <end position="282"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007581"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF02900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABF10273.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="296" mass="31776" checksum="788E4AEC5A2B14E3" modified="2006-05-30" version="1">MNTMLPVLYVSHGSPMLAIDPGPTGAAFDALGVRLRALKPKAVLVISAHWIYSTLAVSTRSQQEAWHDFGGFPRELYTLRYDAPGSPELAGRVKALVEAAQIPGAGFVCEDEERPLDHGAWMPLRHFFPDADVPVVQLAFNPYLPPATQIEIGRALAPLREEGVLVIGSGSFTHNLREVFGAGERKAQHGPATEPYVDAFRDWMSGALDEALATGDVSRIADYRAQAPFARRAHPTDEHLLPFYVALGAAIGPASVAEGPARVDRVADEVTYGVLAMDTFVFDGIGTGHAPLSKAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="123" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LHX2</accession>
    <name>Q1LHX2_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Flavohemoprotein</fullName>
            <ecNumber evidence="6">1.14.12.17</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="18">Flavohemoglobin</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="17">Hemoglobin-like protein</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="19">Nitric oxide dioxygenase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="24" type="primary">hmp</name>
        <name evidence="24" type="ordered locus">Rmet_3382</name>
    </gene>
    <organism evidence="24 25">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="25" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="25">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="16">Is involved in NO detoxification in an aerobic process, termed nitric oxide dioxygenase (NOD) reaction that utilizes O(2) and NAD(P)H to convert NO to nitrate, which protects the bacterium from various noxious nitrogen compounds. Therefore, plays a central role in the inducible response to nitrosative stress.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>NADH + 2 nitric oxide + 2 O2 = H(+) + NAD(+) + 2 nitrate</text>
            <dbReference type="Rhea" id="RHEA:19469"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16480"/>
            <dbReference type="ChEBI" id="CHEBI:17632"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.14.12.17"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>NADPH + 2 nitric oxide + 2 O2 = H(+) + NADP(+) + 2 nitrate</text>
            <dbReference type="Rhea" id="RHEA:19465"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16480"/>
            <dbReference type="ChEBI" id="CHEBI:17632"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.14.12.17"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>heme b</name>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the globin family. Two-domain flavohemoproteins subfamily.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">In the C-terminal section; belongs to the flavoprotein pyridine nucleotide cytochrome reductase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="20">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="1.14.12.17" evidence="6"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10254.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3382"/>
    <dbReference type="EnsemblBacteria" id="ABF10254">
        <property type="protein sequence ID" value="ABF10254"/>
        <property type="gene ID" value="Rmet_3382"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3382"/>
    <dbReference type="eggNOG" id="COG1017">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG1018">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003827_12_0_4"/>
    <dbReference type="OMA" id="EICAAWG"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008941">
        <property type="term" value="F:nitric oxide dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019825">
        <property type="term" value="F:oxygen binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005344">
        <property type="term" value="F:oxygen carrier activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051409">
        <property type="term" value="P:response to nitrosative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009636">
        <property type="term" value="P:response to toxic substance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.490.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008333">
        <property type="entry name" value="Cbr1-like_FAD-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017927">
        <property type="entry name" value="FAD-bd_FR_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001709">
        <property type="entry name" value="Flavoprot_Pyr_Nucl_cyt_Rdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039261">
        <property type="entry name" value="FNR_nucleotide-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000971">
        <property type="entry name" value="Globin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009050">
        <property type="entry name" value="Globin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012292">
        <property type="entry name" value="Globin/Proto"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023950">
        <property type="entry name" value="Hmp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001433">
        <property type="entry name" value="OxRdtase_FAD/NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017938">
        <property type="entry name" value="Riboflavin_synthase-like_b-brl"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43396:SF3">
        <property type="entry name" value="PTHR43396:SF3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00970">
        <property type="entry name" value="FAD_binding_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00042">
        <property type="entry name" value="Globin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00175">
        <property type="entry name" value="NAD_binding_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00371">
        <property type="entry name" value="FPNCR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46458">
        <property type="entry name" value="SSF46458"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52343">
        <property type="entry name" value="SSF52343"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63380">
        <property type="entry name" value="SSF63380"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51384">
        <property type="entry name" value="FAD_FR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01033">
        <property type="entry name" value="GLOBIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0216">Detoxification</keyword>
    <keyword evidence="24" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="12" id="KW-0274">FAD</keyword>
    <keyword evidence="10" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="9 21" id="KW-0349">Heme</keyword>
    <keyword evidence="14" id="KW-0408">Iron</keyword>
    <keyword evidence="11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-0520">NAD</keyword>
    <keyword evidence="13" id="KW-0521">NADP</keyword>
    <keyword evidence="24" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="21" id="KW-0561">Oxygen transport</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="21" id="KW-0813">Transport</keyword>
    <feature type="domain" description="GLOBIN" evidence="22">
        <location>
            <begin position="38"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="domain" description="FAD-binding FR-type" evidence="23">
        <location>
            <begin position="172"/>
            <end position="282"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001762"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00001970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006401"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00008414"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00014637"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022575"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00025094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00030024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00030929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00033187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="RuleBase" id="RU000356"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS01033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS51384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="EMBL" id="ABF10254.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="429" mass="46974" checksum="AB62FEEB37E4ED25" modified="2006-05-30" version="1">MSSAGFIVCKNMHFTCMIQGVLLMLSAASRPYIDASVPVLREHGLAITTHFYREMFAARPELKRIFNMGNQANGSQQQSLASAVFAYAANIDRADVLAPVVERIVHKHVAVGLTPAHYPIVGKYLLEAIAAVLGDAATPPLLAAWDEAYWLLAGELIAAEARLYERHGVAAGELLRVRVVNREQEGDQVVALTLAAEDGSPLRDFAPGQYISVEASFADGRRQLRQYSLSGERGLPTWRISVKREDGASDAPAGTVSNWLHENAQVGTTLHVSTPWGDFAPELDSRQPIVLMSAGIGVTPMVSVLRTLAHENPHRPVLFAHAARHGRQHAHRRDLAWARERMPNLRTHVSYETPQSVDASGRDYDHAGTMPVEALLKSGDNAAFNDARFYLCGPLGFMQEQRRALIASGVPVGHIHREVFGPDLLDDIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-10-07" version="50" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LHZ3</accession>
    <name>Q1LHZ3_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_3361</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10233.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3361"/>
    <dbReference type="EnsemblBacteria" id="ABF10233">
        <property type="protein sequence ID" value="ABF10233"/>
        <property type="gene ID" value="Rmet_3361"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3361"/>
    <dbReference type="HOGENOM" id="CLU_445972_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="59"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="94"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="161"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="190"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="220"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="278"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="320"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="357"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="384"/>
            <end position="408"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF10233.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="559" mass="59918" checksum="330A80DFBBE7B130" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LIE7</accession>
    <name>Q1LIE7_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Aldehyde dehydrogenase (NAD(+))</fullName>
            <ecNumber evidence="3">1.2.1.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Rmet_3207</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="1">Homotetramer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the aldehyde dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.2.1.3" evidence="3"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10079.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3207"/>
    <dbReference type="EnsemblBacteria" id="ABF10079">
        <property type="protein sequence ID" value="ABF10079"/>
        <property type="gene ID" value="Rmet_3207"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3207"/>
    <dbReference type="eggNOG" id="COG1012">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005391_1_2_4"/>
    <dbReference type="OMA" id="DAWKVYM"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004029">
        <property type="term" value="F:aldehyde dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043878">
        <property type="term" value="F:glyceraldehyde-3-phosphate dehydrogenase (NAD+) (non-phosphorylating) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.309.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.605.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016161">
        <property type="entry name" value="Ald_DH/histidinol_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016163">
        <property type="entry name" value="Ald_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029510">
        <property type="entry name" value="Ald_DH_CS_GLU"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016162">
        <property type="entry name" value="Ald_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015590">
        <property type="entry name" value="Aldehyde_DH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044638">
        <property type="entry name" value="ALDH7A1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43521">
        <property type="entry name" value="PTHR43521"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00171">
        <property type="entry name" value="Aldedh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53720">
        <property type="entry name" value="SSF53720"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00687">
        <property type="entry name" value="ALDEHYDE_DEHYDR_GLU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 5 7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aldedh" evidence="6">
        <location>
            <begin position="28"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="active site" evidence="4">
        <location>
            <position position="261"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00024226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABF10079.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="507" mass="53729" checksum="10E67C0617847580" modified="2006-05-30" version="1">MKAQEIAACWQAMDLPPPWTPAEGAAPGVLAVRSPIDGEVFGQLAMCDAALANARIARAHAAQTTWALTPAPARGEVVRRFGEALRANKDALGRLVSLESGKILQEGLGEVQEMIDICDFAVGLSRQLHGLTIASERPAHAMRETWHPYGLCGVISAFNFPVAVWAWNAALALVCGNGVVWKPSEKTPLCALAAQGLLDRVFERHAPQFDGLSCVLTGGRQLGEHLVQHPAVRLMSATGSTRMGREVGMACAAHFKRAILELGGNNAAIVTPSAELEMTIRAMTFAAAGTAGQRCTTLRRAFVHADLIETVTNRLRQVFARLPVGDPLEDGTLVGPLIDHAAGDTMANALASCRAQGNVVHGGERLLGDRYPHACYVRPALVLTDAQHDTMLTETFAPILYLMPYTSLDEAIALNNAASHGLSSCIFTESLREAERFLSSAGSDCGIANVNIGTSGAEIGGAFGGEKATGGGRESGSDAWKGYMRRATNTINYGDALPLAQGIRFDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LIR8</accession>
    <name>Q1LIR8_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Extra-cytoplasmic Solute Receptor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">bug</name>
        <name evidence="4" type="ordered locus">Rmet_3086</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Periplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the UPF0065 (bug) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09958.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3086"/>
    <dbReference type="EnsemblBacteria" id="ABF09958">
        <property type="protein sequence ID" value="ABF09958"/>
        <property type="gene ID" value="Rmet_3086"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3086"/>
    <dbReference type="eggNOG" id="COG3181">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045683_0_0_4"/>
    <dbReference type="OMA" id="NSPDMQK"/>
    <dbReference type="OrthoDB" id="447734at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042597">
        <property type="term" value="C:periplasmic space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005064">
        <property type="entry name" value="BUG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042100">
        <property type="entry name" value="Bug_dom1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42928">
        <property type="entry name" value="PTHR42928"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03401">
        <property type="entry name" value="TctC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017082">
        <property type="entry name" value="YflP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0675">Receptor</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193153" evidence="3">
        <location>
            <begin position="35"/>
            <end position="332"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF09958.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="332" mass="34998" checksum="1D292AC8B880F0F6" modified="2006-05-30" version="1">MRVKSPENNEETPLKPSRLSLAALLLAAATTAHAAWPDRPIKLIVPFPPGQATDIFARALAEKLGKQLGQSVIVENRAGAGGNIGMEVAAKAPADGYTLVMAGSAMAINQTLYKKVNYDPRQDFAPISGVFSVPLVFLATPQSGFTSLRDLITKAKAAPGKYSYASAGIGGTQHLSAEMLKAQTGIFVVHIPYKGSGPAQADFLGNQIPLMVDSVTAAMPLIQSHKALPLAVTTTQRVPQLPSVPTVREQGVPGFEALGWAVMMAPAGTPPAVVSQLNAETVKALQSPELHKFITDRGSEPMPMTPAETGKFVDSEIRKWATVVKRSGATAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="64" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LIT4</accession>
    <name>Q1LIT4_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Outer membrane lipoprotein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_3070</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09942.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517570.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3070"/>
    <dbReference type="EnsemblBacteria" id="ABF09942">
        <property type="protein sequence ID" value="ABF09942"/>
        <property type="gene ID" value="Rmet_3070"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3070"/>
    <dbReference type="eggNOG" id="COG3133">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_090265_3_2_4"/>
    <dbReference type="OMA" id="DEVTIYM"/>
    <dbReference type="OrthoDB" id="1934896at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193541" evidence="1">
        <location>
            <begin position="21"/>
            <end position="155"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF09942.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="155" mass="15462" checksum="A187CC48C7DE102A" modified="2006-05-30" version="1">MNKILRIGGVAVLAAATLLAGCSTQSNSNSVYTGGQAQREQTVRFGVVEGVRDVMIQGEATGAGTLAGGALGGVAAGSTIGGGNGAIAAGLLGAILGGMAGSAAENKIAQRRGLEITVRLENGEMRAITQDADEIFRPGDRVRLLSSGGVTRVTH</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-01-15" modified="2022-08-03" version="96" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LJ94</accession>
    <name>MIAA_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">tRNA dimethylallyltransferase</fullName>
            <ecNumber evidence="1">2.5.1.75</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Dimethylallyl diphosphate:tRNA dimethylallyltransferase</fullName>
            <shortName evidence="1">DMAPP:tRNA dimethylallyltransferase</shortName>
            <shortName evidence="1">DMATase</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Isopentenyl-diphosphate:tRNA isopentenyltransferase</fullName>
            <shortName evidence="1">IPP transferase</shortName>
            <shortName evidence="1">IPPT</shortName>
            <shortName evidence="1">IPTase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">miaA</name>
        <name type="ordered locus">Rmet_2909</name>
    </gene>
    <organism>
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 in tRNAs that read codons beginning with uridine, leading to the formation of N6-(dimethylallyl)adenosine (i(6)A).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>adenosine(37) in tRNA + dimethylallyl diphosphate = diphosphate + N(6)-dimethylallyladenosine(37) in tRNA</text>
            <dbReference type="Rhea" id="RHEA:26482"/>
            <dbReference type="Rhea" id="RHEA-COMP:10162"/>
            <dbReference type="Rhea" id="RHEA-COMP:10375"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57623"/>
            <dbReference type="ChEBI" id="CHEBI:74411"/>
            <dbReference type="ChEBI" id="CHEBI:74415"/>
            <dbReference type="EC" id="2.5.1.75"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="1">Monomer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the IPP transferase family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.75" evidence="1"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09782.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517456.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q1LJ94"/>
    <dbReference type="SMR" id="Q1LJ94"/>
    <dbReference type="STRING" id="266264.Rmet_2909"/>
    <dbReference type="EnsemblBacteria" id="ABF09782">
        <property type="protein sequence ID" value="ABF09782"/>
        <property type="gene ID" value="Rmet_2909"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2909"/>
    <dbReference type="eggNOG" id="COG0324">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032616_0_0_4"/>
    <dbReference type="OMA" id="YAKRQYT"/>
    <dbReference type="OrthoDB" id="1069591at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052381">
        <property type="term" value="F:tRNA dimethylallyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008033">
        <property type="term" value="P:tRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00185">
        <property type="entry name" value="IPP_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039657">
        <property type="entry name" value="Dimethylallyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018022">
        <property type="entry name" value="IPT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11088">
        <property type="entry name" value="PTHR11088"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00174">
        <property type="entry name" value="miaA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <keyword id="KW-0819">tRNA processing</keyword>
    <feature type="chain" id="PRO_1000020649" description="tRNA dimethylallyltransferase">
        <location>
            <begin position="1"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="region of interest" description="Interaction with substrate tRNA" evidence="1">
        <location>
            <begin position="39"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="region of interest" description="Interaction with substrate tRNA" evidence="1">
        <location>
            <begin position="163"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="region of interest" description="Interaction with substrate tRNA" evidence="1">
        <location>
            <begin position="250"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="14"/>
            <end position="21"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="16"/>
            <end position="21"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Interaction with substrate tRNA" evidence="1">
        <location>
            <position position="105"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with substrate tRNA" evidence="1">
        <location>
            <position position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00185"/>
        </source>
    </evidence>
    <sequence length="315" mass="34490" checksum="958282C0AFA893D0" modified="2006-05-30" version="1">MSVQSHPPVVCLLGPTASGKTAAALELAARTPVEIISLDSALVYREMDIGTAKPTHEELASVPHHLIDIIDPLESYSAAQFMTDAERLIGEIRARGRTPLIVGGTMLYYKALTQGLNDLPQADATVRAELDALAAERGWPALHAMLAEVDPITAARLAPNDAQRIQRALEIHRLSGQPMSALLARQADARTFTGAADARYRVIALEPSDRAVLHARIADRYDAMLKGGLIEEVERLRARGDLHLGLPSIRCVGYRQVWEYLDGDCDFATMRERGIAATRQLCKRQLTWLRSTPERIGIDCLAPDYVARVADAAGW</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2007-09-11" modified="2022-05-25" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LJB0</accession>
    <name>CLPS_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">ATP-dependent Clp protease adapter protein ClpS</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">clpS</name>
        <name type="ordered locus">Rmet_2893</name>
    </gene>
    <organism>
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in the modulation of the specificity of the ClpAP-mediated ATP-dependent protein degradation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Binds to the N-terminal domain of the chaperone ClpA.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the ClpS family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09766.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008644942.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q1LJB0"/>
    <dbReference type="SMR" id="Q1LJB0"/>
    <dbReference type="STRING" id="266264.Rmet_2893"/>
    <dbReference type="EnsemblBacteria" id="ABF09766">
        <property type="protein sequence ID" value="ABF09766"/>
        <property type="gene ID" value="Rmet_2893"/>
    </dbReference>
    <dbReference type="GeneID" id="60820671"/>
    <dbReference type="KEGG" id="rme:Rmet_2893"/>
    <dbReference type="eggNOG" id="COG2127">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_134358_2_1_4"/>
    <dbReference type="OMA" id="NDDYTSM"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030163">
        <property type="term" value="P:protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1390.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00302">
        <property type="entry name" value="ClpS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022935">
        <property type="entry name" value="ClpS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003769">
        <property type="entry name" value="ClpS_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014719">
        <property type="entry name" value="Ribosomal_L7/12_C/ClpS-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33473">
        <property type="entry name" value="PTHR33473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02617">
        <property type="entry name" value="ClpS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54736">
        <property type="entry name" value="SSF54736"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000300721" description="ATP-dependent Clp protease adapter protein ClpS">
        <location>
            <begin position="1"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00302"/>
        </source>
    </evidence>
    <sequence length="108" mass="12295" checksum="1F357E1F890E393E" modified="2006-05-30" version="1">MATRLANTPQRETGTVIERKEQQLKPPAMYKVVLLNDDYTPMEFVVMILQQYFSRDRETATQIMLTVHREGKGVCGIYTRDIAATKVELVSTHARQAGHPLQCVMEEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LJR5</accession>
    <name>Q1LJR5_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">1-acylglycerol-3-phosphate O-acyltransferase</fullName>
            <ecNumber evidence="2">2.3.1.51</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">plsC</name>
        <name evidence="2" type="ordered locus">Rmet_2738</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.3.1.51" evidence="2"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09611.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517312.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2738"/>
    <dbReference type="EnsemblBacteria" id="ABF09611">
        <property type="protein sequence ID" value="ABF09611"/>
        <property type="gene ID" value="Rmet_2738"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2738"/>
    <dbReference type="eggNOG" id="COG0204">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027938_0_1_4"/>
    <dbReference type="OMA" id="PMLWVSN"/>
    <dbReference type="OrthoDB" id="1381894at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003841">
        <property type="term" value="F:1-acylglycerol-3-phosphate O-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002123">
        <property type="entry name" value="Plipid/glycerol_acylTrfase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01553">
        <property type="entry name" value="Acyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00563">
        <property type="entry name" value="PlsC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PlsC" evidence="1">
        <location>
            <begin position="66"/>
            <end position="178"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF09611.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="278" mass="30187" checksum="93C2AD132B639389" modified="2006-05-30" version="1">MIWLRKARLTFHLLRGVLFCATVFPWASARLRERLIRAWSRKLLAICGIEVEVLGLAPGQAAPKGAMLVSNHISWLDIYVIHSWQPVRFVAKSEIRNWPVVGWLCDKTGTIFIERARKRDAHRVLHHITEVMQQGDLVCVFPEGTTTDGSKVLPFHANLMQAPVAGKLPVQPVGLSYLDAATGKPTLAPAYIDDISLVQCLNAILKSPPIKARLVIGPQLHAASAGRRELAESAREVVTHLVEGAHEAAHGAAEAFHSLPVAATPPVLTSVAAPAAGH</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-12-12" modified="2022-08-03" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LJV5</accession>
    <name>UBID_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">3-octaprenyl-4-hydroxybenzoate carboxy-lyase</fullName>
            <ecNumber evidence="1">4.1.1.98</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Polyprenyl p-hydroxybenzoate decarboxylase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">ubiD</name>
        <name type="ordered locus">Rmet_2698</name>
    </gene>
    <organism>
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the decarboxylation of 3-octaprenyl-4-hydroxy benzoate to 2-octaprenylphenol, an intermediate step in ubiquinone biosynthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a 4-hydroxy-3-all-trans-polyprenylbenzoate + H(+) = a 2-all-trans-polyprenylphenol + CO2</text>
            <dbReference type="Rhea" id="RHEA:41680"/>
            <dbReference type="Rhea" id="RHEA-COMP:9514"/>
            <dbReference type="Rhea" id="RHEA-COMP:9516"/>
            <dbReference type="ChEBI" id="CHEBI:1269"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:78396"/>
            <dbReference type="EC" id="4.1.1.98"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>prenyl-FMN</name>
            <dbReference type="ChEBI" id="CHEBI:87746"/>
        </cofactor>
        <text evidence="1">Binds 1 prenylated FMN (prenyl-FMN) per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; ubiquinone biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homohexamer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the UbiD family.</text>
    </comment>
    <comment type="sequence caution" evidence="2">
        <conflict type="erroneous initiation">
            <sequence resource="EMBL-CDS" id="ABF09571" version="1"/>
        </conflict>
    </comment>
    <dbReference type="EC" id="4.1.1.98" evidence="1"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09571.1"/>
        <property type="status" value="ALT_INIT"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_029309737.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q1LJV5"/>
    <dbReference type="SMR" id="Q1LJV5"/>
    <dbReference type="STRING" id="266264.Rmet_2698"/>
    <dbReference type="EnsemblBacteria" id="ABF09571">
        <property type="protein sequence ID" value="ABF09571"/>
        <property type="gene ID" value="Rmet_2698"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2698"/>
    <dbReference type="eggNOG" id="COG0043">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023348_4_1_4"/>
    <dbReference type="OrthoDB" id="1012819at2"/>
    <dbReference type="UniPathway" id="UPA00232"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008694">
        <property type="term" value="F:3-octaprenyl-4-hydroxybenzoate carboxy-lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006744">
        <property type="term" value="P:ubiquinone biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01636">
        <property type="entry name" value="UbiD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002830">
        <property type="entry name" value="UbiD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023677">
        <property type="entry name" value="UbiD_bacteria"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30108">
        <property type="entry name" value="PTHR30108"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01977">
        <property type="entry name" value="UbiD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00148">
        <property type="entry name" value="TIGR00148"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0210">Decarboxylase</keyword>
    <keyword id="KW-0285">Flavoprotein</keyword>
    <keyword id="KW-0288">FMN</keyword>
    <keyword id="KW-0456">Lyase</keyword>
    <keyword id="KW-0464">Manganese</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0831">Ubiquinone biosynthesis</keyword>
    <feature type="chain" id="PRO_0000267687" description="3-octaprenyl-4-hydroxybenzoate carboxy-lyase">
        <location>
            <begin position="1"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="1">
        <location>
            <position position="302"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="177"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="180"/>
            <end position="182"/>
        </location>
        <ligand>
            <name>prenyl-FMN</name>
            <dbReference type="ChEBI" id="CHEBI:87746"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="194"/>
            <end position="196"/>
        </location>
        <ligand>
            <name>prenyl-FMN</name>
            <dbReference type="ChEBI" id="CHEBI:87746"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="199"/>
            <end position="200"/>
        </location>
        <ligand>
            <name>prenyl-FMN</name>
            <dbReference type="ChEBI" id="CHEBI:87746"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="243"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01636"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="507" mass="56699" checksum="B7D263F063A883FB" modified="2006-12-12" version="2">MQYKDLRDFMSQLEQRGELKRVAAPVSPNLEMTEICDRLLRANGPAVLFEQPRRDNGDIYKVPVLANLFGTTQRVAQGMGASSLEDLRDIGRVLSVLKEPEPPRGLREAGKLFTLAKSVWDMAPKRVSSPACQEIVWEGNDVDLARLPIQTCWPGDAAPLITWGLVVTKGPHKKRQNLGIYRQQVISRNQVIMRWLAHRGGALDFREHALANPGKPFPIAVALGADPATILGAVTPVPDTLSEYQFAGLLRGSRTALASCITPTLSELSVPASAEIILEGHIQPDPNHPSGYQHALEGPYGDHTGYYNEQDWFPVFTIDRITMRRDPIYHSTYTGKPPDEPAVLGVALNEVFVPLLQKQFPEITDFYLPPEGCSYRMALVRMKKQYAGHAKRVMFGVWSFLRQFMYTKFIVVVDDDIDVRDWKEVIWAITTRVDPTRDTVMVDNTPIDYLDFASPVSGLGSKMGIDATDKWPGETTREWGTPITMAPEIKARVDQMWGSLFEEGPGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LK18</accession>
    <name>Q1LK18_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 6">UTP--glucose-1-phosphate uridylyltransferase</fullName>
            <ecNumber evidence="3 6">2.7.7.9</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">UDP-glucose pyrophosphorylase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">galU</name>
        <name evidence="8" type="ordered locus">Rmet_2631</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 6">
            <text>alpha-D-glucose 1-phosphate + H(+) + UTP = diphosphate + UDP-alpha-D-glucose</text>
            <dbReference type="Rhea" id="RHEA:19889"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:46398"/>
            <dbReference type="ChEBI" id="CHEBI:58601"/>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
            <dbReference type="EC" id="2.7.7.9"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the UDPGP type 2 family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.9" evidence="3 6"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09508.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517207.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2631"/>
    <dbReference type="EnsemblBacteria" id="ABF09508">
        <property type="protein sequence ID" value="ABF09508"/>
        <property type="gene ID" value="Rmet_2631"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2631"/>
    <dbReference type="eggNOG" id="COG1210">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029499_1_2_4"/>
    <dbReference type="OMA" id="LCAEHFI"/>
    <dbReference type="OrthoDB" id="1402673at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003983">
        <property type="term" value="F:UTP:glucose-1-phosphate uridylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009058">
        <property type="term" value="P:biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006011">
        <property type="term" value="P:UDP-glucose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02541">
        <property type="entry name" value="UGPase_prokaryotic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005771">
        <property type="entry name" value="GalU_uridylyltTrfase_bac/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005835">
        <property type="entry name" value="NTP_transferase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43197">
        <property type="entry name" value="PTHR43197"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00483">
        <property type="entry name" value="NTP_transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="SSF53448"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01099">
        <property type="entry name" value="galU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 6 8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="NTP_transferase" evidence="7">
        <location>
            <begin position="12"/>
            <end position="269"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000872"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006890"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012415"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00019048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU361259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ABF09508.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="299" mass="33050" checksum="C20D2038A362B762" modified="2006-05-30" version="1">MENRVTKAVFPVAGLGTRFLPATKASPKEMLPVVDKPLIQYAVEEAMAAGITEMIFVTGRSKRAIEDHFDKAYELEAELEAKNKQALLDVVRSIKPANVECYYVRQPEALGLGHAVLCAAKLVGDTPFAVMLADDLLDGGKDLPVMKQMVDIYNHYNCSVLGVEEIAPEQSRSYGVIDGREWDDRVIKMSAIVEKPAPEKAPSNLGVVGRYILTPRIFDHIRELKPGAGGEIQLTDAIQSMLDQEQVLAYRYKGVRYDCGSKLGYLKATVEFALRHPEVRDEFSAYLSQRCTSPSSVPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="51" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LKF5</accession>
    <name>Q1LKF5_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_2494</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09371.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008649341.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2494"/>
    <dbReference type="EnsemblBacteria" id="ABF09371">
        <property type="protein sequence ID" value="ABF09371"/>
        <property type="gene ID" value="Rmet_2494"/>
    </dbReference>
    <dbReference type="GeneID" id="60821032"/>
    <dbReference type="KEGG" id="rme:Rmet_2494"/>
    <dbReference type="eggNOG" id="ENOG502Z8BP">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038130_0_0_4"/>
    <dbReference type="OMA" id="VHLQAHV"/>
    <dbReference type="OrthoDB" id="453935at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021292">
        <property type="entry name" value="DUF2863"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11062">
        <property type="entry name" value="DUF2863"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF09371.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="400" mass="43812" checksum="5F7A04682F53C53D" modified="2006-05-30" version="1">MAGFRPKSSARLSPDAERLVADALALDASGSRMEDGYWERRLSQRLHRLLKNGSQTALDAALEHLFKHNADASDVLAEQAETLAESATIEVDGAKLDVLLIAAPILAHTRYAIPSGALKPELAQTLAVHLQAHVLATGTKVALSPYLYSIDQLPRSHSDTFALTHKLATAALAGTMPKVDLRDLPETAPILADPRYLLAVVVAPHEGALFRWQEDAKEHHAERSVCLEQWRQQVQPTVALLLPGCEFELLLPDAFFLSCRESDKSIRPLTVRAAVNYLCGTLDVPAGQLAAVVAGFGEEVVEEYRVGFTMRGEKEVIYGIVWPVYGREAGEADAEEKGNPVEQICEELRNAGVEDIFRHAALFDPEYCEDCGTPLYADRSGEIVHAELPEDAPTQQPLFH</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LKK6</accession>
    <name>Q1LKK6_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Molybdopterin molybdenumtransferase</fullName>
            <ecNumber evidence="7">2.10.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">moeA</name>
        <name evidence="9" type="ordered locus">Rmet_2443</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2 7">Catalyzes the insertion of molybdate into adenylated molybdopterin with the concomitant release of AMP.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>adenylyl-molybdopterin + H(+) + molybdate = AMP + H2O + Mo-molybdopterin</text>
            <dbReference type="Rhea" id="RHEA:35047"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:36264"/>
            <dbReference type="ChEBI" id="CHEBI:62727"/>
            <dbReference type="ChEBI" id="CHEBI:71302"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="2.10.1.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="7">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3 7">Cofactor biosynthesis; molybdopterin biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 7">Belongs to the MoeA family.</text>
    </comment>
    <dbReference type="EC" id="2.10.1.1" evidence="7"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09320.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517037.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2443"/>
    <dbReference type="EnsemblBacteria" id="ABF09320">
        <property type="protein sequence ID" value="ABF09320"/>
        <property type="gene ID" value="Rmet_2443"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2443"/>
    <dbReference type="eggNOG" id="COG0303">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010186_7_0_4"/>
    <dbReference type="OMA" id="VDHVHPT"/>
    <dbReference type="OrthoDB" id="651103at2"/>
    <dbReference type="UniPathway" id="UPA00344"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061599">
        <property type="term" value="F:molybdopterin molybdotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006777">
        <property type="term" value="P:Mo-molybdopterin cofactor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032324">
        <property type="term" value="P:molybdopterin cofactor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00887">
        <property type="entry name" value="MoeA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.340.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.980.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036425">
        <property type="entry name" value="MoaB/Mog-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001453">
        <property type="entry name" value="MoaB/Mog_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038987">
        <property type="entry name" value="MoeA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005111">
        <property type="entry name" value="MoeA_C_domain_IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036688">
        <property type="entry name" value="MoeA_C_domain_IV_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005110">
        <property type="entry name" value="MoeA_linker/N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036135">
        <property type="entry name" value="MoeA_linker/N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10192">
        <property type="entry name" value="PTHR10192"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00994">
        <property type="entry name" value="MoCF_biosynth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03454">
        <property type="entry name" value="MoeA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03453">
        <property type="entry name" value="MoeA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00852">
        <property type="entry name" value="MoCF_biosynth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53218">
        <property type="entry name" value="SSF53218"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63867">
        <property type="entry name" value="SSF63867"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63882">
        <property type="entry name" value="SSF63882"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00177">
        <property type="entry name" value="molyb_syn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 7" id="KW-0500">Molybdenum</keyword>
    <keyword evidence="6 7" id="KW-0501">Molybdenum cofactor biosynthesis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="MoCF_biosynth" evidence="8">
        <location>
            <begin position="198"/>
            <end position="339"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002901"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005046"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022505"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU365090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ABF09320.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="428" mass="45104" checksum="4D1B35609EFBDFB0" modified="2006-05-30" version="1">MPSLQSVISCLSDYDPSALPVAKANAIIGDVIEPVGGIEQLAIRTALDRVLAEDVISSIDVPAHDNSAMDGYAFSSAALADAGSDVVLQVIGTAYAGNALGSPAGPGQAVRIMTGAVMPDGCDTVVPQEFVEIQAGGNQIRFAADAVRAGDNRRLRGEDLASGQAALAAGRILQPADIGLLASLGVAEVKVRRRLRVAFFSTGDELRSIGEVLDPGCVYDSNRYTLHGMLRRLNVDLIDMGVVRDDPEALEAAFRSACENADAIITSGGVSVGEADYTKQIMAQLGDVTFWKIAMRPGRPMAFGRITSNGREALLFGLPGNPVAVMVTFYHFVRAALHRLMGAKVAPLPLMRVRSAGTIRKKPGRTEYQRGILAPGADGTLEVRVTGQQGSGVLRSMSEANCFIVLAHEQGTVEAGESVEIMLFDGLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LL00</accession>
    <name>Q1LL00_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Single-stranded DNA-binding protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">ssb2</name>
        <name evidence="5" type="ordered locus">Rmet_2297</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09176.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_003098976.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2297"/>
    <dbReference type="EnsemblBacteria" id="ABF09176">
        <property type="protein sequence ID" value="ABF09176"/>
        <property type="gene ID" value="Rmet_2297"/>
    </dbReference>
    <dbReference type="GeneID" id="56409806"/>
    <dbReference type="GeneID" id="61526425"/>
    <dbReference type="KEGG" id="rme:Rmet_2297"/>
    <dbReference type="eggNOG" id="COG0629">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_142244_1_0_4"/>
    <dbReference type="OMA" id="RMERAPW"/>
    <dbReference type="OrthoDB" id="1942216at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04496">
        <property type="entry name" value="SSB_OBF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000424">
        <property type="entry name" value="Primosome_PriB/ssb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011344">
        <property type="entry name" value="ssDNA-bd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00436">
        <property type="entry name" value="SSB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002070">
        <property type="entry name" value="SSB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50935">
        <property type="entry name" value="SSB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2 3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="118"/>
            <end position="146"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRNR" id="PIRNR002070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00252"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABF09176.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="146" mass="16947" checksum="02C8CA84BB58806B" modified="2006-05-30" version="1">MSTQFIGEGNIGSPPEYREFPNGNDDPRRLLRLNVYFDNPVPTKGGEFEDRGGFWAPVELWHHDADRWQQLYQKGMRVLVVGRMERDPWTDNEDQPRETWQVNARSVGILPYRIESVALSPKPQEAEPKPQATQESTAPKETKRRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="59" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LL02</accession>
    <name>Q1LL02_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_2295</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09174.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_003116836.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2295"/>
    <dbReference type="EnsemblBacteria" id="ABF09174">
        <property type="protein sequence ID" value="ABF09174"/>
        <property type="gene ID" value="Rmet_2295"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2295"/>
    <dbReference type="eggNOG" id="COG1475">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_082088_1_1_4"/>
    <dbReference type="OMA" id="IEQNEAW"/>
    <dbReference type="OrthoDB" id="877442at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014996">
        <property type="entry name" value="AcaB"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08900">
        <property type="entry name" value="AcaB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03761">
        <property type="entry name" value="ICE_PFL4669"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="220"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="72"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF09174.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="263" mass="28616" checksum="19999D6DCC95D456" modified="2006-05-30" version="1">MATSNEPLQLNLGSLRSAMSLTLHTHHASRIWHGRAAAEGRPGIVGLNGYIAVMNKMKRGSEQDDPYSDWWMLRIEEKLDQTRTTLQSLREQVDQALAGVPAALSLGENLNVQPVKLPLFVNAQLGFAAVYLLADYDDIARKLILAHHTALIDRSTLERWLNEGAHALRSLFSLAQQYRYSGCTRDDFAAKNAAARAALEKFGELPQDVLEGMRRSKFAPPVVRRGLQQRGDSAAAAASPTDEGAAADSAEAKSTAAGEDEPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="67" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LL16</accession>
    <name>Q1LL16_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_2281</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09160.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008642389.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2281"/>
    <dbReference type="EnsemblBacteria" id="ABF09160">
        <property type="protein sequence ID" value="ABF09160"/>
        <property type="gene ID" value="Rmet_2281"/>
    </dbReference>
    <dbReference type="GeneID" id="60821126"/>
    <dbReference type="KEGG" id="rme:Rmet_2281"/>
    <dbReference type="eggNOG" id="COG3193">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_103773_1_2_4"/>
    <dbReference type="OMA" id="NNWAVTI"/>
    <dbReference type="OrthoDB" id="1551899at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005624">
        <property type="entry name" value="PduO/GlcC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038084">
        <property type="entry name" value="PduO/GlcC-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03928">
        <property type="entry name" value="Haem_degrading"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143744">
        <property type="entry name" value="SSF143744"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF09160.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="135" mass="14013" checksum="0C2F9BE7E0F69F28" modified="2006-05-30" version="1">MQQKTVLTADDVKKVMAAAEAEALANNWAVTIVVVDDGGHPLAMQRLDGVAPISAYIATEKARTASLGRRESKIYEDMINNGRYSFMTAPVLNGMLEGGVPIVANGQVVGAVGVSGVKSTEDAQIARAGIASLGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LL35</accession>
    <name>Q1LL35_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative signaling protein (GGDEF domain)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_2262</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09141.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516964.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2262"/>
    <dbReference type="EnsemblBacteria" id="ABF09141">
        <property type="protein sequence ID" value="ABF09141"/>
        <property type="gene ID" value="Rmet_2262"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2262"/>
    <dbReference type="eggNOG" id="COG2199">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_11_16_4"/>
    <dbReference type="OMA" id="TPEYDIL"/>
    <dbReference type="OrthoDB" id="1635706at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd01949">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000160">
        <property type="entry name" value="GGDEF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029787">
        <property type="entry name" value="Nucleotide_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00990">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00267">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55073">
        <property type="entry name" value="SSF55073"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00254">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50887">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GGDEF" evidence="2">
        <location>
            <begin position="106"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="42"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF09141.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="237" mass="26494" checksum="85C6013ED4592818" modified="2006-05-30" version="1">MPEPDLFESENSVLAHARSVFCDVDAGEEAYRHTLGQLISYYERLMRETRRLIRRSDREELEMNRLNHRLQELAQELEYRATHDTLTGALNRGAVIDRATAALARGSLALIVIDIDHFKRLNDDFGHPVGDAVICGVTACLKDTVRMEGVIGRMGGEEFAALLPDFTIDAAATLADQMRQAIAGHRFPAPVNREVTASFGVSWSPQGRPFEHAYGRADEALYEAKRGGRNRVTQARE</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="57" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LL74</accession>
    <name>Q1LL74_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_2223</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09102.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516930.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2223"/>
    <dbReference type="EnsemblBacteria" id="ABF09102">
        <property type="protein sequence ID" value="ABF09102"/>
        <property type="gene ID" value="Rmet_2223"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2223"/>
    <dbReference type="eggNOG" id="ENOG5032KMV">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1265198_0_0_4"/>
    <dbReference type="OMA" id="VRESDWT"/>
    <dbReference type="OrthoDB" id="1944453at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF09102.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="229" mass="24901" checksum="6294FF6F0550D542" modified="2006-05-30" version="1">MSLDFLPADAGPGFAVGAAKPWTGRYPVSSGLFEVRPARMPRDVLPVFDDDLDCIVGYQRAFADRCHLFDLNGDMLGVWEAMTALPPPAERGSLMVVGGLWKANVRGMTALGLNGSGMALAPAMLAQLRSRFAALADTPLLYAEAAHARMSDPQRFVPVHILRLAMRHGERIAPPEGLKGVARYMIGLRIRQLPFMLDLVTAHAGATIVAFNYWRYADTSPVARSRGNP</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-01-15" modified="2022-08-03" version="92" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LLK1</accession>
    <name>PURA_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Adenylosuccinate synthetase</fullName>
            <shortName evidence="1">AMPSase</shortName>
            <shortName evidence="1">AdSS</shortName>
            <ecNumber evidence="1">6.3.4.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">IMP--aspartate ligase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">purA</name>
        <name type="ordered locus">Rmet_2096</name>
    </gene>
    <organism>
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Plays an important role in the de novo pathway of purine nucleotide biosynthesis. Catalyzes the first committed step in the biosynthesis of AMP from IMP.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>GTP + IMP + L-aspartate = GDP + 2 H(+) + N(6)-(1,2-dicarboxyethyl)-AMP + phosphate</text>
            <dbReference type="Rhea" id="RHEA:15753"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57567"/>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="EC" id="6.3.4.4"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="1">Binds 1 Mg(2+) ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Purine metabolism; AMP biosynthesis via de novo pathway; AMP from IMP: step 1/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the adenylosuccinate synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.3.4.4" evidence="1"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08975.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="RefSeq" id="WP_011516809.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
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    <dbReference type="EnsemblBacteria" id="ABF08975">
        <property type="protein sequence ID" value="ABF08975"/>
        <property type="gene ID" value="Rmet_2096"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2096"/>
    <dbReference type="eggNOG" id="COG0104">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029848_0_0_4"/>
    <dbReference type="OMA" id="FHHAKPI"/>
    <dbReference type="OrthoDB" id="232152at2"/>
    <dbReference type="UniPathway" id="UPA00075">
        <property type="reaction ID" value="UER00335"/>
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    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="F:adenylosuccinate synthase activity"/>
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        <property type="project" value="UniProtKB-UniRule"/>
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        <property type="term" value="F:GTP binding"/>
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        <property type="term" value="F:magnesium ion binding"/>
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        <property type="project" value="UniProtKB-UniRule"/>
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    <dbReference type="GO" id="GO:0044208">
        <property type="term" value="P:'de novo' AMP biosynthetic process"/>
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        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="Adenylosuccin_syn_GTP-bd"/>
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        <property type="entry name" value="P-loop_NTPase"/>
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        <property type="entry name" value="PTHR11846"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="purA"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ADENYLOSUCCIN_SYN_1"/>
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    </dbReference>
    <dbReference type="PROSITE" id="PS00513">
        <property type="entry name" value="ADENYLOSUCCIN_SYN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0342">GTP-binding</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0658">Purine biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_1000000901" description="Adenylosuccinate synthetase">
        <location>
            <begin position="1"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="1">
        <location>
            <position position="21"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="1">
        <location>
            <position position="49"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="20"/>
            <end position="26"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="1">
        <location>
            <begin position="21"/>
            <end position="24"/>
        </location>
        <ligand>
            <name>IMP</name>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="21"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="1">
        <location>
            <begin position="46"/>
            <end position="49"/>
        </location>
        <ligand>
            <name>IMP</name>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="48"/>
            <end position="50"/>
        </location>
        <ligand>
            <name>GTP</name>
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        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="48"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
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        <location>
            <position position="137"/>
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        <ligand>
            <name>IMP</name>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
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        </ligand>
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    <feature type="binding site" evidence="1">
        <location>
            <position position="151"/>
        </location>
        <ligand>
            <name>IMP</name>
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            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
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        <location>
            <position position="232"/>
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        <location>
            <position position="247"/>
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        <ligand>
            <name>IMP</name>
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        <ligand>
            <name>substrate</name>
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        <location>
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        <ligand>
            <name>IMP</name>
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        <location>
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        <location>
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        <ligand>
            <name>GTP</name>
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        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00011"/>
        </source>
    </evidence>
    <sequence length="447" mass="48261" checksum="5011F52189E80010" modified="2006-05-30" version="1">MSASAVGQGRNVVVIGTQWGDEGKGKIVDWLTDHAKGVVRFQGGHNAGHTLIIGGKKTILRLIPSGIMRESTVCYIGNGVVLSPEALFREIEELESAGLQVQNRLRISEAATLILPYHVAIDKAREARRGAAKIGTTGRGIGPAYEDKVARRALRVQDLFDPKHFAERLRENVEFHNFMLTQYLGAEAVDYQQILDESLAFAPRLKPMVADVSAELYAVNAAGNNLMFEGAQGTLLDVDHGTYPFVTSSNCVAGAAAAGAGVGPGRLNYILGITKAYCTRVGAGPFPSELYDNDNPARQDAVGVRLANVGKEFGSVTGRPRRTGWLDAAALKRSVQINGVSGLCMTKLDVLDGLETIKLCVGYELDGKRVDILPRGSDAVAACVPVYEEFPGWNESTFGVKTWDALPEAARVYLKRIEEVVGIPIDMVSTGPDRDETILLRHPYQVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LLK2</accession>
    <name>Q1LLK2_CUPMC</name>
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        <submittedName>
            <fullName evidence="2">Phosphoribosyltransferase (PRTase)</fullName>
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        </submittedName>
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        <name evidence="2" type="ordered locus">Rmet_2095</name>
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        </lineage>
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        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
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                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
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    <dbReference type="OMA" id="WEFNQIV"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="Pribosyltran"/>
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    </dbReference>
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        <property type="match status" value="1"/>
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    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Pribosyltran" evidence="1">
        <location>
            <begin position="28"/>
            <end position="174"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF08974.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="202" mass="23053" checksum="F6B0165451B48AF1" modified="2006-05-30" version="1">MLYPLAIDTRKDAMNLPINDDENLWVSWDDYHRLIARLALNVHESGWKFDQILCLARGGLRVGDQMSRIFDVPLAILATSSYREAAGTQQGELDIAQYITMTRGNLSGRILLVDDLVDSGVTLERVGRHLKERYPEVTDVRSAVLWYKACSKVTPDYHVTFLPSNPWIHQPFEEYDTLRPHNLAAWLKRGKRASLQEDDSAA</sequence>
</entry>
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    <accession>Q1LLL3</accession>
    <name>Q1LLL3_CUPMC</name>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
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    <gene>
        <name evidence="2" type="ordered locus">Rmet_2084</name>
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    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
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            <taxon>Burkholderiaceae</taxon>
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        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
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                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
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            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="ABF08963.1"/>
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    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2084"/>
    <dbReference type="EnsemblBacteria" id="ABF08963">
        <property type="protein sequence ID" value="ABF08963"/>
        <property type="gene ID" value="Rmet_2084"/>
    </dbReference>
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    <dbReference type="eggNOG" id="ENOG502ZESX">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_034656_1_0_4"/>
    <dbReference type="OMA" id="MHISRAS"/>
    <dbReference type="OrthoDB" id="1869640at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="16"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="51"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="85"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="116"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="152"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="189"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="225"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="255"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="285"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="318"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="374"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="399"/>
            <end position="416"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF08963.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="444" mass="46295" checksum="74600D95400FD792" modified="2006-05-30" version="1">MVKAVNLDYASAPRPGLVFGCLMPAPWLGVVAGLLLVLGPVDALPDRFAPLALAAVHALALGMLLPVMLGALFQMMPVVAGVSVPFAGLVSPFVAAAGAGTAAALAAGFLGSGATAFRWAVATGGTFLTLAGLLLLAAGLRVAAVDATTRTLAWIGAPLIVTALGGAALAGLFGGMWTVAPEQVLALHVAWGLGGWLAALVSGVATTVLPMFWQTERLPSWLERCLPYGHWLLLLIFSIVVIARPGGAVERFALVPWLLFAMVLGACALKAVWRARRRHDPQWVLWPVSALSLLLAALLVTASALMPDQMPAAAQWCIGVLALIGGGVLPVNAMLGKIVPFLVWLHLRRLLPPRARVPAMQVIISPDSQKRQGWMVLAAFLVLLAVPLAPGLLAKVGGVLFAITNGWLGVQLLRALRCMRVMRKQGGLPPRVAAAVAEHGVPPN</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LLY0</accession>
    <name>Q1LLY0_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Aspartate aminotransferase</fullName>
            <ecNumber evidence="2">2.6.1.1</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">aatA</name>
        <name evidence="2" type="ordered locus">Rmet_1967</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.6.1.1" evidence="2"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08846.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1967"/>
    <dbReference type="EnsemblBacteria" id="ABF08846">
        <property type="protein sequence ID" value="ABF08846"/>
        <property type="gene ID" value="Rmet_1967"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1967"/>
    <dbReference type="eggNOG" id="COG0436">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017584_4_2_4"/>
    <dbReference type="OMA" id="FGFECPP"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004069">
        <property type="term" value="F:L-aspartate:2-oxoglutarate aminotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009058">
        <property type="term" value="P:biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="SSF53383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aminotran_1_2" evidence="1">
        <location>
            <begin position="78"/>
            <end position="439"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF08846.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="453" mass="50377" checksum="C3799501E0D1C2A3" modified="2006-05-30" version="1">MPPGLPRLASLIADAPPDAQDNPEIPRFRCRQRPAACPDIANTAVKPIQKSHKLQNVCYDIRGPVLEKAKQMEEEGHKIIKLNIGNLAVFGFDAPEEIQQDMMRNLPNSAGYSDSKGIFAARKAIMHYTQEKKIQGVGLDDIYVGNGASELIVMAMNALLNSGDEMLVPAPDYPLWTAAVSLSGGTPVHYVCDEANEWMPDLDDIRRKITPNTRGIVIINPNNPTGALYSDELLKEIVAIAREHGLIIFADEIYDKVLYDGNTHTSIGSLSTDVLTVTFNGLSKNYRSCGYRAGWMVVSGDKRPALDYIEGLNMLSSMRLCANVPGQWAIQTALGGYQSINDLVTEGGRLRRQRDLAYELITKIPGVTCVKPKAALYLFPKLDLSMYPIQDDQEFIYELLQESKVLLVQGTGFNWGAPDHFRIVFLPHEEDLREAITRVGRFLESYRKRHGTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="97" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LM67</accession>
    <name>Q1LM67_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Putative transporter, ATP-binding component (ABC superfamily)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">ybbA</name>
        <name evidence="9" type="ordered locus">Rmet_1880</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08759.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1880"/>
    <dbReference type="EnsemblBacteria" id="ABF08759">
        <property type="protein sequence ID" value="ABF08759"/>
        <property type="gene ID" value="Rmet_1880"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1880"/>
    <dbReference type="eggNOG" id="COG4181">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_22_4"/>
    <dbReference type="OMA" id="FARAIMS"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd03255">
        <property type="entry name" value="ABC_MJ0796_LolCDE_FtsE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017911">
        <property type="entry name" value="MacB_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 7 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="1" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-1278">Translocase</keyword>
    <feature type="domain" description="ABC transporter" evidence="8">
        <location>
            <begin position="1"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="5"/>
            <end position="12"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ABF08759.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="195" mass="20988" checksum="622096C42BE3C100" modified="2006-05-30" version="1">MAIVGASGSGKSTLLGLLAGLDLPSTGSVRLHGQDLFRLDEDQRAALRGRHVGFVFQSFQLVGHLTALENVMLPLELRGETDHVRERAVDMLERVGLGQRLSHYPRTLSGGEQQRVALARAFVARPDILFADEPTGSLDTATGEAVIALMFELNRDAGSTLVLVTHDRSVAARCGRILTIEAGRVASDEFMDVGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="66" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LML3</accession>
    <name>Q1LML3_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Putative esterase of the alpha/beta hydrolase fold family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_1734</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08613.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008644746.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1734"/>
    <dbReference type="ESTHER" id="ralme-q1lml3">
        <property type="family name" value="Hydrolase_RBBP9_YdeN"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF08613">
        <property type="protein sequence ID" value="ABF08613"/>
        <property type="gene ID" value="Rmet_1734"/>
    </dbReference>
    <dbReference type="GeneID" id="60822385"/>
    <dbReference type="KEGG" id="rme:Rmet_1734"/>
    <dbReference type="eggNOG" id="COG3545">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_088863_3_0_4"/>
    <dbReference type="OMA" id="APLEHDK"/>
    <dbReference type="OrthoDB" id="721431at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010662">
        <property type="entry name" value="Hydrolase_RBBP9/YdeN"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06821">
        <property type="entry name" value="Ser_hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="SSF53474"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF08613.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="189" mass="20911" checksum="F55C446B904CFBA3" modified="2006-05-30" version="1">MDFLADTTVLFVPGLRDHVPEHWQTLLQAQIPNSRSVQPLEHDKLSRAARVAALDEALAEIDGPVVLVAHSAGVMITVHWAAQHHRKIRAALLAAPADLENPMPAGYPTHATLDEHGWLPIPRRPLPFPSIVGASRNDPLARFDRVEQMARDWGSELVDLGEVGHLNPAAGYGEWPYAMTLVERLMRRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="96" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LN10</accession>
    <name>Q1LN10_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Periplasmic L-asparaginase II</fullName>
            <ecNumber evidence="12">3.5.1.38</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">ansB</name>
        <name evidence="12" type="ordered locus">Rmet_1583</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the asparaginase 1 family.</text>
    </comment>
    <dbReference type="EC" id="3.5.1.38" evidence="12"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08466.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516318.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1583"/>
    <dbReference type="EnsemblBacteria" id="ABF08466">
        <property type="protein sequence ID" value="ABF08466"/>
        <property type="gene ID" value="Rmet_1583"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1583"/>
    <dbReference type="eggNOG" id="COG0252">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019134_1_2_4"/>
    <dbReference type="OMA" id="RKNHTSR"/>
    <dbReference type="OrthoDB" id="1659551at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0110165">
        <property type="term" value="C:cellular anatomical entity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004067">
        <property type="term" value="F:asparaginase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050417">
        <property type="term" value="F:glutamin-(asparagin-)ase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006528">
        <property type="term" value="P:asparagine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd08964">
        <property type="entry name" value="L-asparaginase_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004550">
        <property type="entry name" value="AsnASE_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036152">
        <property type="entry name" value="Asp/glu_Ase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006034">
        <property type="entry name" value="Asparaginase/glutaminase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020827">
        <property type="entry name" value="Asparaginase/glutaminase_AS1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027475">
        <property type="entry name" value="Asparaginase/glutaminase_AS2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040919">
        <property type="entry name" value="Asparaginase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027473">
        <property type="entry name" value="L-asparaginase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027474">
        <property type="entry name" value="L-asparaginase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037152">
        <property type="entry name" value="L-asparaginase_N_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00710">
        <property type="entry name" value="Asparaginase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17763">
        <property type="entry name" value="Asparaginase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001220">
        <property type="entry name" value="L-ASNase_gatD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00139">
        <property type="entry name" value="ASNGLNASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00870">
        <property type="entry name" value="Asparaginase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53774">
        <property type="entry name" value="SSF53774"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00520">
        <property type="entry name" value="asnASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00144">
        <property type="entry name" value="ASN_GLN_ASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00917">
        <property type="entry name" value="ASN_GLN_ASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51732">
        <property type="entry name" value="ASN_GLN_ASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193637" evidence="9">
        <location>
            <begin position="27"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="domain" description="Asparaginase" evidence="10">
        <location>
            <begin position="50"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="domain" description="Asparaginase_C" evidence="11">
        <location>
            <begin position="263"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="4">
        <location>
            <begin position="139"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="active site" evidence="6">
        <location>
            <position position="59"/>
        </location>
    </feature>
    <feature type="active site" description="Acyl-ester intermediate" evidence="5">
        <location>
            <position position="59"/>
        </location>
    </feature>
    <feature type="active site" description="O-isoaspartyl threonine intermediate" evidence="3">
        <location>
            <position position="59"/>
        </location>
    </feature>
    <feature type="active site" evidence="7">
        <location>
            <position position="139"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="106"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010518"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR001220-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR001220-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR604550-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10099"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10100"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU004456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00710"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF17763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ABF08466.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="375" mass="39692" checksum="7BD6029D698098AF" modified="2006-05-30" version="1">MQRTARLKPLAALLLSASLLTGVAHAQLSPAPAASANAPAGTTAEARKANIVIIGTGGTIAGAGAAATNTAAYQSAVVPVDKIIASVPEISKIANVKGEQVFQIGSESFNNERLIKLAKRVSELLKQPDVDGIVITHGTDTIEETAYFLNLTLKSDKPVVVVGSMRPGTALGADGALNLYDAVLVASNPASKGKGTLVVLNDEIQTGRDVTKTNTFKVETFRSPFGPLGYVVEGRTLYFRLPARPHTTQTEFDIDKIDKLPEVAVVYAYGNMNPAAIDAAVKSGAKAIIFDATGNGSVGDYMVEPLKAARAKGVFIVRASRTGSGVVIRNGEQPDDKYDWIVTDDQIAQKARILMALALTKTNDPKALQEIFWKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LN43</accession>
    <name>Q1LN43_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">P-type conjugative transfer ATPase TrbB (CMGI-2)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">trbB</name>
        <name evidence="4" type="ordered locus">Rmet_1550</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the GSP E family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08433.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1550"/>
    <dbReference type="EnsemblBacteria" id="ABF08433">
        <property type="protein sequence ID" value="ABF08433"/>
        <property type="gene ID" value="Rmet_1550"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1550"/>
    <dbReference type="eggNOG" id="COG4962">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005379_3_0_4"/>
    <dbReference type="OMA" id="PEITFFK"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014149">
        <property type="entry name" value="Conjug-transfer_TrbB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001482">
        <property type="entry name" value="T2SS/T4SS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00437">
        <property type="entry name" value="T2SSE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02782">
        <property type="entry name" value="TrbB_P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="T2SP_E" evidence="3">
        <location>
            <begin position="34"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="334"/>
            <end position="358"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006611"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF08433.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="358" mass="38075" checksum="CF3949B3B1D69579" modified="2006-05-30" version="1">MSARISIEGQSMSATSLDRRIQMLRTAMGPLIAAALEDPDVVEVMLNPDRTLWVDRLSSGRAPMGVELPEADGERIIRLVAAHVGAEVHRGQPLLSAELPETGERFEGILPPAAPGPAFALRKRAIGVIPLERYVVEGMMTAAQAGFLVRAVRERQNVLIAGATSSGKTTLANALLAEIAATGDRVLVLEDTVELQCAARDHVPLRTRAGVVSMTELVRSSMRLRPDRVVVGEVRGAEALDLIKVWGTGHPGGIATIHAGSALGALLRLEQLILEVAVNPPRALIAEAVNVVIHIAGRGRKRRIESIARVVGFDGVGYRLVDWEADALDTPFPELPPLPDAAPAAATFPSPDSLGELP</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="69" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LNA9</accession>
    <name>Q1LNA9_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative transcriptional regulator (DNA binding protein)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_1484</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08367.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1484"/>
    <dbReference type="EnsemblBacteria" id="ABF08367">
        <property type="protein sequence ID" value="ABF08367"/>
        <property type="gene ID" value="Rmet_1484"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1484"/>
    <dbReference type="eggNOG" id="COG3311">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_140176_9_0_4"/>
    <dbReference type="OMA" id="GVVEPHR"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009061">
        <property type="entry name" value="DNA-bd_dom_put_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041657">
        <property type="entry name" value="HTH_17"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12728">
        <property type="entry name" value="HTH_17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46955">
        <property type="entry name" value="SSF46955"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH_17" evidence="2">
        <location>
            <begin position="37"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="87"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="92"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF08367.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="108" mass="12013" checksum="EAF898F559FDE8F7" modified="2006-05-30" version="1">MRCVAGALKAMEVLTMRPAPLRPAAAPATTTAQPQRYLTNDEAADYLRLSPRTLEKQRVLGGGPRFRKFGRRVMYAVADLDAWAAERSFESTSDPEYAEQHSADSRAR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-04-14" modified="2022-08-03" version="105" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LND0</accession>
    <name>GUAA_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">GMP synthase [glutamine-hydrolyzing]</fullName>
            <ecNumber evidence="1">6.3.5.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">GMP synthetase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Glutamine amidotransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">guaA</name>
        <name type="ordered locus">Rmet_1463</name>
    </gene>
    <organism>
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the synthesis of GMP from XMP.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + H2O + L-glutamine + XMP = AMP + diphosphate + GMP + 2 H(+) + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:11680"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57464"/>
            <dbReference type="ChEBI" id="CHEBI:58115"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.3.5.2"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Purine metabolism; GMP biosynthesis; GMP from XMP (L-Gln route): step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <dbReference type="EC" id="6.3.5.2" evidence="1"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08346.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516212.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q1LND0"/>
    <dbReference type="SMR" id="Q1LND0"/>
    <dbReference type="STRING" id="266264.Rmet_1463"/>
    <dbReference type="MEROPS" id="C26.957"/>
    <dbReference type="EnsemblBacteria" id="ABF08346">
        <property type="protein sequence ID" value="ABF08346"/>
        <property type="gene ID" value="Rmet_1463"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1463"/>
    <dbReference type="eggNOG" id="COG0518">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG0519">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014340_0_5_4"/>
    <dbReference type="OMA" id="KRKIIGH"/>
    <dbReference type="OrthoDB" id="504464at2"/>
    <dbReference type="UniPathway" id="UPA00189">
        <property type="reaction ID" value="UER00296"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003922">
        <property type="term" value="F:GMP synthase (glutamine-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016462">
        <property type="term" value="F:pyrophosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006541">
        <property type="term" value="P:glutamine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01742">
        <property type="entry name" value="GATase1_GMP_Synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01997">
        <property type="entry name" value="GMP_synthase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00344">
        <property type="entry name" value="GMP_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017926">
        <property type="entry name" value="GATASE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001674">
        <property type="entry name" value="GMP_synth_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004739">
        <property type="entry name" value="GMP_synth_GATase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022955">
        <property type="entry name" value="GMP_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025777">
        <property type="entry name" value="GMPS_ATP_PPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022310">
        <property type="entry name" value="NAD/GMP_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00117">
        <property type="entry name" value="GATase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00958">
        <property type="entry name" value="GMP_synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02540">
        <property type="entry name" value="NAD_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="SSF52317"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00884">
        <property type="entry name" value="guaA_Cterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00888">
        <property type="entry name" value="guaA_Nterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51273">
        <property type="entry name" value="GATASE_TYPE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51553">
        <property type="entry name" value="GMPS_ATP_PPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword id="KW-0332">GMP biosynthesis</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0658">Purine biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_1000120375" description="GMP synthase [glutamine-hydrolyzing]">
        <location>
            <begin position="1"/>
            <end position="539"/>
        </location>
    </feature>
    <feature type="domain" description="Glutamine amidotransferase type-1" evidence="1">
        <location>
            <begin position="4"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="domain" description="GMPS ATP-PPase" evidence="1">
        <location>
            <begin position="203"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="1">
        <location>
            <position position="81"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="176"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="178"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="230"/>
            <end position="236"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00344"/>
        </source>
    </evidence>
    <sequence length="539" mass="59573" checksum="6F9D01FD69B797F8" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-05-26" modified="2022-08-03" version="95" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LNH3</accession>
    <name>DAPE_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Succinyl-diaminopimelate desuccinylase</fullName>
            <shortName evidence="1">SDAP desuccinylase</shortName>
            <ecNumber evidence="1">3.5.1.18</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">N-succinyl-LL-2,6-diaminoheptanedioate amidohydrolase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">dapE</name>
        <name type="ordered locus">Rmet_1420</name>
    </gene>
    <organism>
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the hydrolysis of N-succinyl-L,L-diaminopimelic acid (SDAP), forming succinate and LL-2,6-diaminoheptanedioate (DAP), an intermediate involved in the bacterial biosynthesis of lysine and meso-diaminopimelic acid, an essential component of bacterial cell walls.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>H2O + N-succinyl-(2S,6S)-2,6-diaminoheptanedioate = (2S,6S)-2,6-diaminoheptanedioate + succinate</text>
            <dbReference type="Rhea" id="RHEA:22608"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:57609"/>
            <dbReference type="ChEBI" id="CHEBI:58087"/>
            <dbReference type="EC" id="3.5.1.18"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <cofactor evidence="1">
            <name>Co(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:48828"/>
        </cofactor>
        <text evidence="1">Binds 2 Zn(2+) or Co(2+) ions per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-lysine biosynthesis via DAP pathway; LL-2,6-diaminopimelate from (S)-tetrahydrodipicolinate (succinylase route): step 3/3.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase M20A family. DapE subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.5.1.18" evidence="1"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08303.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516174.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q1LNH3"/>
    <dbReference type="SMR" id="Q1LNH3"/>
    <dbReference type="STRING" id="266264.Rmet_1420"/>
    <dbReference type="EnsemblBacteria" id="ABF08303">
        <property type="protein sequence ID" value="ABF08303"/>
        <property type="gene ID" value="Rmet_1420"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1420"/>
    <dbReference type="eggNOG" id="COG0624">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021802_4_0_4"/>
    <dbReference type="OMA" id="LNSHHDT"/>
    <dbReference type="OrthoDB" id="275025at2"/>
    <dbReference type="UniPathway" id="UPA00034">
        <property type="reaction ID" value="UER00021"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050897">
        <property type="term" value="F:cobalt ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009014">
        <property type="term" value="F:succinyl-diaminopimelate desuccinylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019877">
        <property type="term" value="P:diaminopimelate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009089">
        <property type="term" value="P:lysine biosynthetic process via diaminopimelate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01690">
        <property type="entry name" value="DapE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001261">
        <property type="entry name" value="ArgE/DapE_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036264">
        <property type="entry name" value="Bact_exopeptidase_dim_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005941">
        <property type="entry name" value="DapE_proteobac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002933">
        <property type="entry name" value="Peptidase_M20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011650">
        <property type="entry name" value="Peptidase_M20_dimer"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07687">
        <property type="entry name" value="M20_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01546">
        <property type="entry name" value="Peptidase_M20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55031">
        <property type="entry name" value="SSF55031"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01246">
        <property type="entry name" value="dapE_proteo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00758">
        <property type="entry name" value="ARGE_DAPE_CPG2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0170">Cobalt</keyword>
    <keyword id="KW-0220">Diaminopimelate biosynthesis</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0457">Lysine biosynthesis</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <feature type="chain" id="PRO_0000375676" description="Succinyl-diaminopimelate desuccinylase">
        <location>
            <begin position="1"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="76"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="1">
        <location>
            <position position="141"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="74"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="107"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="107"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="142"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="170"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="356"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01690"/>
        </source>
    </evidence>
    <sequence length="383" mass="41175" checksum="DAE08D854F3E23E8" modified="2006-05-30" version="1">MNPTLALTEDLIRRRSVTPADEGCQAVLETRLKALGFTCENIVSGPDDFRVTNLWAVKRGTQGTAGKLLAFAGHTDVVPTGPLDQWSSDPFEPSHRDGKLYGRGAADMKTSIAGFVVAVEEFVAKHPEHKGSIAFLITSDEEGPAHDGTVKVVEALKARGERLDYCVVGEPTSVDTLGDMVKNGRRGSLSGKLTVKGVQGHIAYPHLARNPIHLAAPALTTLVNEVWDAGNEYFPPTTWQMSNIHGGTGATNVIPGHVTIDFNFRFSTASTPDGLKSRVHAILDQHGLDYTLDWTLGGEPFLTPRGDLSEALGAAIAAETGVKTELSTTGGTSDGRFIAKICPQVIEFGPPNASIHKIDEHVEVRFVEPLKNVYRGVLERLIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="64" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LNM8</accession>
    <name>Q1LNM8_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_1365</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08248.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516128.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1365"/>
    <dbReference type="EnsemblBacteria" id="ABF08248">
        <property type="protein sequence ID" value="ABF08248"/>
        <property type="gene ID" value="Rmet_1365"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1365"/>
    <dbReference type="eggNOG" id="COG3182">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025664_1_0_4"/>
    <dbReference type="OMA" id="LGRWVYF"/>
    <dbReference type="OrthoDB" id="1656356at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005625">
        <property type="entry name" value="PepSY-ass_TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34219">
        <property type="entry name" value="PTHR34219"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03929">
        <property type="entry name" value="PepSY_TM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="150"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="198"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="358"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="400"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="426"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="451"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="484"/>
            <end position="505"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF08248.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="543" mass="58661" checksum="69FBEBC41A2AEA19" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LNN6</accession>
    <name>Q1LNN6_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Acetyl-CoA acetyltransferase (Acetoacetyl-CoA thiolase)</fullName>
            <ecNumber evidence="7">2.3.1.9</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">phaA</name>
        <name evidence="7" type="ordered locus">Rmet_1357</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the thiolase-like superfamily. Thiolase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.9" evidence="7"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08240.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516121.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1357"/>
    <dbReference type="EnsemblBacteria" id="ABF08240">
        <property type="protein sequence ID" value="ABF08240"/>
        <property type="gene ID" value="Rmet_1357"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1357"/>
    <dbReference type="eggNOG" id="COG0183">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031026_0_0_4"/>
    <dbReference type="OMA" id="ICPSIAI"/>
    <dbReference type="OrthoDB" id="1058688at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003985">
        <property type="term" value="F:acetyl-CoA C-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd00751">
        <property type="entry name" value="thiolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.47.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002155">
        <property type="entry name" value="Thiolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016039">
        <property type="entry name" value="Thiolase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020615">
        <property type="entry name" value="Thiolase_acyl_enz_int_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020610">
        <property type="entry name" value="Thiolase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020617">
        <property type="entry name" value="Thiolase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020613">
        <property type="entry name" value="Thiolase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020616">
        <property type="entry name" value="Thiolase_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02803">
        <property type="entry name" value="Thiolase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00108">
        <property type="entry name" value="Thiolase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000429">
        <property type="entry name" value="Ac-CoA_Ac_transf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53901">
        <property type="entry name" value="SSF53901"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01930">
        <property type="entry name" value="AcCoA-C-Actrans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00098">
        <property type="entry name" value="THIOLASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00737">
        <property type="entry name" value="THIOLASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00099">
        <property type="entry name" value="THIOLASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4 7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Thiolase_N" evidence="5">
        <location>
            <begin position="4"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="domain" description="Thiolase_C" evidence="6">
        <location>
            <begin position="270"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="active site" description="Acyl-thioester intermediate" evidence="3">
        <location>
            <position position="88"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="3">
        <location>
            <position position="349"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="3">
        <location>
            <position position="379"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR000429-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF02803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABF08240.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="393" mass="40726" checksum="E28AEFCDB14B2968" modified="2006-05-30" version="1">MTDIVIVSAARTAVGKFGGSLAKIPAPELGAIVIKAALERAGVKPDQVSEVIMGQVLTAASGQNPARQASRKAGLPDMVPAMTINKVCGSGLKAVMLAANAIVAGEAEIVVAGGMENMSAAPHVLPGSRDGFRMGDTKLVDSMIVDGLWDVYNQYHMGITAENVAKEYGITRQAQDEFAVGSQNKAEAAQKAGKFDEEIVPVLIPQRKGDPVAFKTDEYVRQGATLESMASLKPAFDKTGTVTAANASGLNDGAAAVVVMSAAKARELGLTPLATIKAYANAGVDPAVMGMGPVPASKRCLSRAGWEVKDLDLMEINEAFAAQALAVHQQMGWDQSKINVNGGAIAIGHPIGASGCRILVTLLHEMKRRDAKKGLASLCIGGGMGVALAVERN</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="105" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LP66</accession>
    <name>Q1LP66_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Histidine kinase</fullName>
            <ecNumber evidence="2">2.7.13.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">Rmet_1174</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.</text>
            <dbReference type="EC" id="2.7.13.3"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.7.13.3" evidence="2"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08060.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515954.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1174"/>
    <dbReference type="EnsemblBacteria" id="ABF08060">
        <property type="protein sequence ID" value="ABF08060"/>
        <property type="gene ID" value="Rmet_1174"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1174"/>
    <dbReference type="eggNOG" id="COG2205">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_89_6_4"/>
    <dbReference type="OMA" id="CCGASAW"/>
    <dbReference type="OrthoDB" id="1827824at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00082">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003660">
        <property type="entry name" value="HAMP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003661">
        <property type="entry name" value="HisK_dim/P"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036097">
        <property type="entry name" value="HisK_dim/P_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004358">
        <property type="entry name" value="Sig_transdc_His_kin-like_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00672">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00512">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00344">
        <property type="entry name" value="BCTRLSENSOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00388">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47384">
        <property type="entry name" value="SSF47384"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="SSF55874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50885">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 10" id="KW-0418">Kinase</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="4 10" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193379" description="Histidine kinase" evidence="7">
        <location>
            <begin position="26"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="175"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="domain" description="HAMP" evidence="9">
        <location>
            <begin position="194"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="8">
        <location>
            <begin position="275"/>
            <end position="489"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ABF08060.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="504" mass="54277" checksum="DB1311BE5CFDB98D" modified="2006-05-30" version="1">MNLSLTQRLSAVFAALLLACCGASAWLQVRANAMHEKEVVQDISRGLAAYIARNALLEDQSAIAPQALRQLFGQLMVVNPSVEVYLLDDQGRIRGHDAPAGHLVRDHVNLAPVKKLIDGGALPIFGDDPRSDSGRKVFSAAPLRQGGYIYVVLQGEAHDKLAALASRDAVMRTTLWSMALVAALGLVAGLVAFGLITRPLRRLTLEIRQFDSGDAGDLRRSGIPGAGAADRIQEPMQRDEIAALRTAFRQMADRIETQWRALKQQDQERREMVANISHDLRTPLTSLHGYLETLSLKADKLSAEERQRYLAIALAQSAKVGGLAQSLFELARLEHGMVQAMPETFSIVDLTQDVLEKFALPAEAKHLQLRAGVPPVLPAVTADLGMIERVLTNLLDNAIRHTPVGGTVEVDLTSQNGSVAVTVSDTGPGIPPALRESLFQRPFPSGGAHRGGGLGLLIVQRMLQLNGSQARLVEGQVGTTLRFVLPVAGRESRPAAIADAAATR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LPA6</accession>
    <name>Q1LPA6_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">O-Acetylhomoserine sulfhydrylase</fullName>
            <ecNumber evidence="5">2.5.1.49</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">metY</name>
        <name evidence="5" type="ordered locus">Rmet_1134</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 4">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 4">Belongs to the trans-sulfuration enzymes family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.49" evidence="5"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08020.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1134"/>
    <dbReference type="EnsemblBacteria" id="ABF08020">
        <property type="protein sequence ID" value="ABF08020"/>
        <property type="gene ID" value="Rmet_1134"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1134"/>
    <dbReference type="eggNOG" id="COG2873">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018986_4_0_4"/>
    <dbReference type="OMA" id="YGGMITF"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003961">
        <property type="term" value="F:O-acetylhomoserine aminocarboxypropyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019346">
        <property type="term" value="P:transsulfuration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00614">
        <property type="entry name" value="CGS_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000277">
        <property type="entry name" value="Cys/Met-Metab_PyrdxlP-dep_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006235">
        <property type="entry name" value="OAc-hSer/O-AcSer_sulfhydrylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01053">
        <property type="entry name" value="Cys_Met_Meta_PP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001434">
        <property type="entry name" value="CGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="SSF53383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01326">
        <property type="entry name" value="OAH_OAS_sulfhy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="3">
        <location>
            <position position="224"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR001434-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU362118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABF08020.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="450" mass="48505" checksum="BD29B6E92B58031F" modified="2006-05-30" version="1">MPDGDNHPWLGKPSLFTMSGTRFDTLALHAGAAPDPTTGARATPIHLTTSFVFRDSEHAASLFNMERAGHVYSRISNPTVAVFEERVAALENGAGAIATASGQAALHLAVATLMGAGSHIVASTALYGGSHNLLHYTLRRFGIETTFVNPRDIDAWRAAIRPETKLLFGETLGNPGLDVLDIATVAQIGQEHGIPLLVDSTFTTPYLLRPFDHGAGLLYHSATKFLGGHGTTIGGVLSEGGTFDFQASGRFPELSEPYEGFHNMVFTEESTVAPFLLRARREGLRDFGACMNPMAAWQLLQGIETLPLRMSRHVDNTRRVVQFLASHPMVESVAYPELESHPDYELAKRLLPRGCGAVFSFNLKADRTAGQRFIESLSLFSHLANVGDARSLVIHPASTTHFRMDAAALRAAGIGEGTIRLSIGLEDPDDLIDDLKRGLKAAEKAMGGKA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-01-15" modified="2022-08-03" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LPL1</accession>
    <name>HSCA_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Chaperone protein HscA homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">hscA</name>
        <name type="ordered locus">Rmet_1029</name>
    </gene>
    <organism>
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Chaperone involved in the maturation of iron-sulfur cluster-containing proteins. Has a low intrinsic ATPase activity which is markedly stimulated by HscB.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the heat shock protein 70 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07915.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515822.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q1LPL1"/>
    <dbReference type="SMR" id="Q1LPL1"/>
    <dbReference type="STRING" id="266264.Rmet_1029"/>
    <dbReference type="EnsemblBacteria" id="ABF07915">
        <property type="protein sequence ID" value="ABF07915"/>
        <property type="gene ID" value="Rmet_1029"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1029"/>
    <dbReference type="eggNOG" id="COG0443">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005965_2_1_4"/>
    <dbReference type="OMA" id="PDPHQRR"/>
    <dbReference type="OrthoDB" id="161217at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd10236">
        <property type="entry name" value="HscA_like_NBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.34.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00679">
        <property type="entry name" value="HscA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018181">
        <property type="entry name" value="Heat_shock_70_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042039">
        <property type="entry name" value="HscA_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029048">
        <property type="entry name" value="HSP70_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029047">
        <property type="entry name" value="HSP70_peptide-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013126">
        <property type="entry name" value="Hsp_70_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010236">
        <property type="entry name" value="ISC_FeS_clus_asmbl_HscA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19375">
        <property type="entry name" value="PTHR19375"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00012">
        <property type="entry name" value="HSP70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100920">
        <property type="entry name" value="SSF100920"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100934">
        <property type="entry name" value="SSF100934"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="SSF53067"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01991">
        <property type="entry name" value="HscA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00297">
        <property type="entry name" value="HSP70_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00329">
        <property type="entry name" value="HSP70_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01036">
        <property type="entry name" value="HSP70_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0143">Chaperone</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_1000044880" description="Chaperone protein HscA homolog">
        <location>
            <begin position="1"/>
            <end position="621"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00679"/>
        </source>
    </evidence>
    <sequence length="621" mass="66246" checksum="9A4B3E9F05405A52" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="51" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LPL9</accession>
    <name>Q1LPL9_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_1021</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07907.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008649302.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1021"/>
    <dbReference type="EnsemblBacteria" id="ABF07907">
        <property type="protein sequence ID" value="ABF07907"/>
        <property type="gene ID" value="Rmet_1021"/>
    </dbReference>
    <dbReference type="GeneID" id="60821882"/>
    <dbReference type="KEGG" id="rme:Rmet_1021"/>
    <dbReference type="HOGENOM" id="CLU_2583845_0_0_4"/>
    <dbReference type="OMA" id="YETHEVQ"/>
    <dbReference type="OrthoDB" id="1966574at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF07907.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="79" mass="8884" checksum="947B92A29443D36C" modified="2006-05-30" version="1">MAYSSEWKKRVFETHEIQVLVKPRSPIAWEYTVRVCRKGTNIRAASTMVETAPPSERYGTPEEAEAAGFARGKAIAEQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LPT9</accession>
    <name>Q1LPT9_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Cytochrome bo(3) ubiquinol oxidase subunit 4</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Cytochrome o ubiquinol oxidase subunit 4</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">Oxidase bo(3) subunit 4</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">Ubiquinol oxidase polypeptide IV</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">Ubiquinol oxidase subunit 4</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="primary">cyoD</name>
        <name evidence="16" type="ordered locus">Rmet_0951</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Cytochrome bo(3) ubiquinol terminal oxidase is the component of the aerobic respiratory chain of E.coli that predominates when cells are grown at high aeration. Has proton pump activity across the membrane in addition to electron transfer, pumping 2 protons/electron.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Heterooctamer of two A chains, two B chains, two C chains and two D chains.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the cytochrome c oxidase bacterial subunit 4 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07837.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008643397.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0951"/>
    <dbReference type="EnsemblBacteria" id="ABF07837">
        <property type="protein sequence ID" value="ABF07837"/>
        <property type="gene ID" value="Rmet_0951"/>
    </dbReference>
    <dbReference type="GeneID" id="60825438"/>
    <dbReference type="KEGG" id="rme:Rmet_0951"/>
    <dbReference type="eggNOG" id="COG3125">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_140945_1_1_4"/>
    <dbReference type="OMA" id="IVVHMVF"/>
    <dbReference type="OrthoDB" id="2088013at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009486">
        <property type="term" value="F:cytochrome bo3 ubiquinol oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015990">
        <property type="term" value="P:electron transport coupled proton transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005171">
        <property type="entry name" value="Cyt_c_oxidase_su4_prok"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014210">
        <property type="entry name" value="Cyt_o_ubiqinol_oxidase_su4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03626">
        <property type="entry name" value="COX4_pro"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02847">
        <property type="entry name" value="CyoD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 15" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="20"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="50"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="83"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008079"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011700"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00014689"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00025694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00030071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00030211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00031887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00032185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="ABF07837.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="116" mass="12893" checksum="F0DAD5A938491A1A" modified="2006-05-30" version="1">MAQQHASTAAHGASHGHSSVKSYVIGFVLAVILTVIPFKIVMDGTMERGTMLWIILGMAAVQIVVHLKYFLHLDGSNGQRWNVMALLFTVLILFIVLAGSLWIMHNMNANMMPWMK</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LPY7</accession>
    <name>Q1LPY7_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 18">Diacylglycerol kinase</fullName>
            <ecNumber evidence="5 18">2.7.1.107</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="primary">dgkA</name>
        <name evidence="19" type="ordered locus">Rmet_0903</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="18">Catalyzes the ATP-dependent phosphorylation of sn-l,2-diacylglycerol (DAG) to phosphatidic acid. Involved in the recycling of diacylglycerol produced as a by-product during membrane-derived oligosaccharide (MDO) biosynthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>a 1,2-diacyl-sn-glycerol + ATP = a 1,2-diacyl-sn-glycero-3-phosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10272"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17815"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58608"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.107"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3 18">Cell inner membrane</location>
            <topology evidence="3 18">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 18">Belongs to the bacterial diacylglycerol kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.107" evidence="5 18"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07789.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0903"/>
    <dbReference type="EnsemblBacteria" id="ABF07789">
        <property type="protein sequence ID" value="ABF07789"/>
        <property type="gene ID" value="Rmet_0903"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0903"/>
    <dbReference type="eggNOG" id="COG0818">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_112343_3_0_4"/>
    <dbReference type="OMA" id="KCAWVEE"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004143">
        <property type="term" value="F:diacylglycerol kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006654">
        <property type="term" value="P:phosphatidic acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd14264">
        <property type="entry name" value="DAGK_IM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.3610">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000829">
        <property type="entry name" value="DAGK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033718">
        <property type="entry name" value="DAGK_prok"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036945">
        <property type="entry name" value="DAGK_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34299">
        <property type="entry name" value="PTHR34299"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01219">
        <property type="entry name" value="DAGK_prokar"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14 18" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 18" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="7" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="13 18 19" id="KW-0418">Kinase</keyword>
    <keyword evidence="18" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="15" id="KW-0460">Magnesium</keyword>
    <keyword evidence="17 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12 18" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-1208">Phospholipid metabolism</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 18 19" id="KW-0808">Transferase</keyword>
    <keyword evidence="10 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="16 18" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="50"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="73"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="115"/>
            <end position="136"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004429"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00017575"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="RuleBase" id="RU363065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="ABF07789.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="144" mass="15553" checksum="B771030EAE3BC0EE" modified="2006-05-30" version="1">MAAEVESEYSIASNPHKGNRGLTRAWHAAINSASGLRYAILEESAFRQELTLVVILTPCAFIIPATVVERVMLLGTLLLVLIVELLNSSVEAAIDRISLERHSLSKRAKDFGSAAVMLALILCGGTWAAIAGPHVVRWVGMLFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="56" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LQ42</accession>
    <name>Q1LQ42_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_0848</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07734.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515677.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF07734">
        <property type="protein sequence ID" value="ABF07734"/>
        <property type="gene ID" value="Rmet_0848"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0848"/>
    <dbReference type="eggNOG" id="ENOG502Z7N9">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_060950_0_0_4"/>
    <dbReference type="OMA" id="KAHEHYR"/>
    <dbReference type="OrthoDB" id="762345at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF07734.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="307" mass="34947" checksum="30841679C3D01926" modified="2006-05-30" version="1">MGVFDLFSKRQKRARGEMPDVYAYDELPNPLRVQIVHIISDAFGTDNYGSDHAGKAYEFVKQTLCREFGVFELIKYPKSDQESVFNFFLQEPSVERALDVVELCFKIIHSFIPDNWSYLNNTRRKLDTAEAVAELNERFKEHGVGYQFESGEIIRVDSEFLHAEAVKPTLAVLRDKGFQGANEEFLKAHEHYRHGRYKECLVDALKAFESTMKTICSLRGWRTQPTDTAKNLISTCMSNGLFPAYFDSQFSSIRSLLESGVPTVRNKNGGHGQGTDPVAVPEYLARYTLNLTATTILFMVEAHQTTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LQ69</accession>
    <name>Q1LQ69_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Phenazine biosynthesis PhzC/PhzF protein</fullName>
            <ecNumber evidence="3">5.1.-.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_0821</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PhzF family.</text>
    </comment>
    <dbReference type="EC" id="5.1.-.-" evidence="3"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07707.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0821"/>
    <dbReference type="EnsemblBacteria" id="ABF07707">
        <property type="protein sequence ID" value="ABF07707"/>
        <property type="gene ID" value="Rmet_0821"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0821"/>
    <dbReference type="eggNOG" id="COG0384">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_048756_0_1_4"/>
    <dbReference type="OMA" id="PGYEMRF"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016853">
        <property type="term" value="F:isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009058">
        <property type="term" value="P:biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003719">
        <property type="entry name" value="Phenazine_PhzF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13774">
        <property type="entry name" value="PTHR13774"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02567">
        <property type="entry name" value="PhzC-PhzF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016184">
        <property type="entry name" value="PhzC_PhzF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00654">
        <property type="entry name" value="PhzF_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0413">Isomerase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" evidence="2">
        <location>
            <position position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR016184-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF07707.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="305" mass="32795" checksum="473574E9B5CB519D" modified="2006-05-30" version="1">MAWRLPYTCRVADFWKAAPMPTFAYRIVNVFAESTFGGNPLCVFEDARGLDDATMQALALQFNLSETTFVLPSDNANAAVRIFTPGYEMRFAGHPTLGTAHVVRDLMKTGDALSLEFKAGVVPVTAKGDRWTLTAPHTGAPKTAPAGLPDAEMASLLGLTMDDLAGSPMWVDTGADQLLVPVKTPDAVRRAQPDSSRLDRWPQSSLGRKTAYVFAFDPEQPGKALVRYFFTKQGGGVAEDPGTGSACANLGGWLVATGHQLPASYEVDQGEFVDRRCRLDLNVTTEGAIRVGGRVIELGRGTVTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LQ92</accession>
    <name>Q1LQ92_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Putative Pirin-related protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Rmet_0798</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the pirin family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07684.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515634.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0798"/>
    <dbReference type="EnsemblBacteria" id="ABF07684">
        <property type="protein sequence ID" value="ABF07684"/>
        <property type="gene ID" value="Rmet_0798"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0798"/>
    <dbReference type="eggNOG" id="COG1741">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045717_5_0_4"/>
    <dbReference type="OMA" id="PHPHRGM"/>
    <dbReference type="OrthoDB" id="833478at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012093">
        <property type="entry name" value="Pirin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008778">
        <property type="entry name" value="Pirin_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003829">
        <property type="entry name" value="Pirin_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011051">
        <property type="entry name" value="RmlC_Cupin_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13903">
        <property type="entry name" value="PTHR13903"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02678">
        <property type="entry name" value="Pirin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05726">
        <property type="entry name" value="Pirin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006232">
        <property type="entry name" value="Pirin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51182">
        <property type="entry name" value="SSF51182"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pirin" evidence="3">
        <location>
            <begin position="38"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="domain" description="Pirin_C" evidence="4">
        <location>
            <begin position="193"/>
            <end position="299"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008416"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU003457"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF02678"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF05726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABF07684.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="307" mass="33131" checksum="50CD1B9851B09122" modified="2006-05-30" version="1">MDTTRIQTVPATATETVQRSRSVDRVVRGIATSDGAGVKLTRVLTQNLQRRLDPFLMLDAFGTDNKDDYIGGFPDHPHRGFETITYMLAGRMRHRDSAGHEGLLENGGAQWMVAGAGVVHSELPEQEDGRMEGFQLWLNLPAADKMTTPWYRDMPASAIPTVALPSGGTVRVLAGASHGVAGAVTRPVTQPLYLDVHLPAGQTFAQTLTAGHNAFLYVYRGAVTVGDADGAVVEAQRMGVLDNATRADGVVIKADEDARFLLIAGQPLNEPIAQYGPFVMNTQDQIYQTLADFRDGRFATIPANQAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="62" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LQP7</accession>
    <name>Q1LQP7_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Flp pilus assembly protein (TadG-like)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_0643</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07529.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515500.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0643"/>
    <dbReference type="EnsemblBacteria" id="ABF07529">
        <property type="protein sequence ID" value="ABF07529"/>
        <property type="gene ID" value="Rmet_0643"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0643"/>
    <dbReference type="eggNOG" id="COG4961">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_136271_1_0_4"/>
    <dbReference type="OMA" id="LIGICEF"/>
    <dbReference type="OrthoDB" id="1451040at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012495">
        <property type="entry name" value="TadE"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07811">
        <property type="entry name" value="TadE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="21"/>
            <end position="45"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF07529.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="152" mass="16534" checksum="5C5EDE8A4E188EF2" modified="2006-05-30" version="1">MKHRAHPLRFARQRGLAAVEFALIAGMFFTLLIGIMEFSRVLFYWNTAAEVTRMAARSAVVCDSGASIIKTRMENMLPLLQDSNISVAYSPTGCDSDPATARSTCQTVTVSVSNVTIATMIPVMRLRIGMPPFTTTMTRESMQTSTGGSVCS</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LQQ1</accession>
    <name>Q1LQQ1_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Bacterial type II secretion system protein, probably involved in Flp pilus synthesis</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">tadC</name>
        <name evidence="8" type="ordered locus">Rmet_0639</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07525.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515496.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0639"/>
    <dbReference type="EnsemblBacteria" id="ABF07525">
        <property type="protein sequence ID" value="ABF07525"/>
        <property type="gene ID" value="Rmet_0639"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0639"/>
    <dbReference type="eggNOG" id="COG2064">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_056917_0_1_4"/>
    <dbReference type="OMA" id="SIKRAWP"/>
    <dbReference type="OrthoDB" id="1559784at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018076">
        <property type="entry name" value="T2SS_GspF_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00482">
        <property type="entry name" value="T2SSF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="104"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="137"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="283"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="domain" description="T2SSF" evidence="7">
        <location>
            <begin position="176"/>
            <end position="304"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00482"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ABF07525.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="323" mass="35612" checksum="EE87631B7322B473" modified="2006-05-30" version="1">MQDIMQGFRADQLIVLALVFVAAFGTVLGVLYVFSPDRMRGRMEQIASETGTVPGNAGQQQAWVEKLVKWAQPVSRLSLPKEGWENSQLRVRFMNAGWRDASAAPLYFAAKTLLAVALPMIGLIATSSVPAFQEQSTTFAVLAVLAAIGYYIPNIVLSRKVTARQRTVFEEFPDVIDLLTVCVEAGLGLDAALMRVADELALRCPVLADELQLMLLELRSGFSKEKALSNLSLRTGVEDVDKFASMLIQADRFGTSLGESLRVLSDMLRTKRRMRAEEQAAKIALKLLFPLIFTIFPTLLLVLLGPAFIQIYRVLLPTMTGTN</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="104" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LQU6</accession>
    <name>Q1LQU6_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Isoquinoline 1-oxidoreductase subunit alpha</fullName>
            <ecNumber evidence="3">1.3.99.16</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">iorA</name>
        <name evidence="3" type="ordered locus">Rmet_0594</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EC" id="1.3.99.16" evidence="3"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07480.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008643561.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0594"/>
    <dbReference type="EnsemblBacteria" id="ABF07480">
        <property type="protein sequence ID" value="ABF07480"/>
        <property type="gene ID" value="Rmet_0594"/>
    </dbReference>
    <dbReference type="GeneID" id="60824191"/>
    <dbReference type="KEGG" id="rme:Rmet_0594"/>
    <dbReference type="eggNOG" id="COG2080">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_052511_3_0_4"/>
    <dbReference type="OMA" id="DVRLLWV"/>
    <dbReference type="OrthoDB" id="1819473at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047121">
        <property type="term" value="F:isoquinoline 1-oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00207">
        <property type="entry name" value="fer2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002888">
        <property type="entry name" value="2Fe-2S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036884">
        <property type="entry name" value="2Fe-2S-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036010">
        <property type="entry name" value="2Fe-2S_ferredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001041">
        <property type="entry name" value="2Fe-2S_ferredoxin-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006058">
        <property type="entry name" value="2Fe2S_fd_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012675">
        <property type="entry name" value="Beta-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00111">
        <property type="entry name" value="Fer2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01799">
        <property type="entry name" value="Fer2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47741">
        <property type="entry name" value="SSF47741"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54292">
        <property type="entry name" value="SSF54292"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00197">
        <property type="entry name" value="2FE2S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51085">
        <property type="entry name" value="2FE2S_FER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="2Fe-2S ferredoxin-type" evidence="2">
        <location>
            <begin position="1"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF07480.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="152" mass="15817" checksum="4056D5773A1EB66A" modified="2006-05-30" version="1">MTSLTINGKTVEVDADPSTPLLWALRDNLGMTGTKFGCGMASCGACTVHINGTATRSCVMPISGTAGSKITTIEAMGDDKVGRAVLAAWLKHDVAQCGYCQNGQVMSAVGLLRSKPRPTDADIDQAMAGNVCRCGTYQRIRAAIKDAAHAIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="58" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LQX6</accession>
    <name>Q1LQX6_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_0564</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07450.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515426.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0564"/>
    <dbReference type="ESTHER" id="ralme-q1lqx6">
        <property type="family name" value="Duf_3141"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF07450">
        <property type="protein sequence ID" value="ABF07450"/>
        <property type="gene ID" value="Rmet_0564"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0564"/>
    <dbReference type="eggNOG" id="COG3243">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014618_0_0_4"/>
    <dbReference type="OMA" id="GHPCYFI"/>
    <dbReference type="OrthoDB" id="400860at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024501">
        <property type="entry name" value="DUF3141"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11339">
        <property type="entry name" value="DUF3141"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="SSF53474"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF07450.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="754" mass="83546" checksum="6AA5BD3A60AD6C5C" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LQZ6</accession>
    <name>Q1LQZ6_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative transcriptional regulator, TetR-family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_0544</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07430.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515406.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0544"/>
    <dbReference type="EnsemblBacteria" id="ABF07430">
        <property type="protein sequence ID" value="ABF07430"/>
        <property type="gene ID" value="Rmet_0544"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0544"/>
    <dbReference type="eggNOG" id="COG1309">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_105787_0_0_4"/>
    <dbReference type="OMA" id="CARIIAF"/>
    <dbReference type="OrthoDB" id="1309950at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001647">
        <property type="entry name" value="HTH_TetR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50977">
        <property type="entry name" value="HTH_TETR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH tetR-type" evidence="2">
        <location>
            <begin position="22"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="H-T-H motif" evidence="1">
        <location>
            <begin position="46"/>
            <end position="65"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF07430.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="222" mass="23933" checksum="5877A57D4BE7D055" modified="2006-05-30" version="1">MNPTIPMQTVEGLRAPRQERSEASLRAMLAAGRGLINASGSLTGFSLAELTQAASTSVGAFYTRFRDKETFCALVLEDMLTELRAGLDAQMAQDPVWHDGPADAICARIIAFYVATFRSYAGVFAAYLRHAASGGPLWGPIRDANQRILDVMVPCLSGHLSAEGRDWAEDEIRMAIRLVVSTLTSIALEVPHGIGMEDAALEPRLAAMLQRYLAGPPHVPAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LRB5</accession>
    <name>Q1LRB5_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative glycerophosphoryl diester phosphodiesterase, periplasmic protein</fullName>
            <ecNumber evidence="2">3.1.4.46</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">glpQ</name>
        <name evidence="2" type="ordered locus">Rmet_0425</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EC" id="3.1.4.46" evidence="2"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07311.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515300.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0425"/>
    <dbReference type="EnsemblBacteria" id="ABF07311">
        <property type="protein sequence ID" value="ABF07311"/>
        <property type="gene ID" value="Rmet_0425"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0425"/>
    <dbReference type="eggNOG" id="COG0584">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030226_0_0_4"/>
    <dbReference type="OMA" id="TFRPENY"/>
    <dbReference type="OrthoDB" id="1542724at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008889">
        <property type="term" value="F:glycerophosphodiester phosphodiesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.190">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030395">
        <property type="entry name" value="GP_PDE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017946">
        <property type="entry name" value="PLC-like_Pdiesterase_TIM-brl"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03009">
        <property type="entry name" value="GDPD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51695">
        <property type="entry name" value="SSF51695"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51704">
        <property type="entry name" value="GP_PDE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GP-PDE" evidence="1">
        <location>
            <begin position="52"/>
            <end position="384"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF07311.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="392" mass="42150" checksum="5C2FFDE933788716" modified="2006-05-30" version="1">MHHPFITRFTLAALTAGLIAGCAAPPRAPTSTAAREAPSTAYATTSAAPKPVLIIGHRGASALRPEHTLASYQKAIDDGADIIEPDLVATKDGVLVARHENDITGTTNVAEMPEFADRKRVKVIDGERLTGWFTEDFTLAELKTLRARERIPKLRPANAKLNDQFEIPTFDEILKLAELAAMKRGRPVGIYPELKHPTYFRGIGLPLEERLVTALQGSAYAKTVPVYVQSFEAAPLRTVKRLLGNSMPNVMIVQLIGNPRSRPADWRLAGDTRTYADMLKPIGLREVATYANGIGPEKNSVIPRDASGNLASPTALLANAHAAGLVVHPYTFRPENAFLPKSLRAPGDDATRNPAGMISEIQTFAAAGVDGVFTDDPALGRRAVDPPANGGH</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LRF0</accession>
    <name>Q1LRF0_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Putative thioesterase Protein</fullName>
            <ecNumber evidence="5">3.1.2.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">yciA</name>
        <name evidence="5" type="ordered locus">Rmet_0390</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the acyl coenzyme A hydrolase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.2.-" evidence="5"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07276.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0390"/>
    <dbReference type="EnsemblBacteria" id="ABF07276">
        <property type="protein sequence ID" value="ABF07276"/>
        <property type="gene ID" value="Rmet_0390"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0390"/>
    <dbReference type="eggNOG" id="COG1607">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_050164_2_0_4"/>
    <dbReference type="OMA" id="ITTQIEV"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016790">
        <property type="term" value="F:thiolester hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040170">
        <property type="entry name" value="Cytosol_ACT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033120">
        <property type="entry name" value="HOTDOG_ACOT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029069">
        <property type="entry name" value="HotDog_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006683">
        <property type="entry name" value="Thioestr_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11049">
        <property type="entry name" value="PTHR11049"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03061">
        <property type="entry name" value="4HBT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54637">
        <property type="entry name" value="SSF54637"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51770">
        <property type="entry name" value="HOTDOG_ACOT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3 5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HotDog ACOT-type" evidence="4">
        <location>
            <begin position="35"/>
            <end position="148"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABF07276.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="157" mass="17070" checksum="70590239697A1DD8" modified="2006-05-30" version="1">MPRLSQRSDNPAGLRFIRPERPAMTTSSFVPELPSGKNPALRVVPMPADANVHGDVFGGWIMSQVDMAGSIPAVERAQGRVATVAVNSFLFKNPVFVGDLVSFYADIVKTGRTSITVSVEVYAQRMRHANEIVKVTEATLTYVATDESRQPRVLPAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LRK3</accession>
    <name>Q1LRK3_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Putative regulatory protein, IclR family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Rmet_0337</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07223.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515218.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0337"/>
    <dbReference type="EnsemblBacteria" id="ABF07223">
        <property type="protein sequence ID" value="ABF07223"/>
        <property type="gene ID" value="Rmet_0337"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0337"/>
    <dbReference type="eggNOG" id="COG1414">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_062618_2_0_4"/>
    <dbReference type="OMA" id="QAHAYLV"/>
    <dbReference type="OrthoDB" id="370329at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029016">
        <property type="entry name" value="GAF-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014757">
        <property type="entry name" value="Tscrpt_reg_IclR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005471">
        <property type="entry name" value="Tscrpt_reg_IclR_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09339">
        <property type="entry name" value="HTH_IclR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01614">
        <property type="entry name" value="IclR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00346">
        <property type="entry name" value="HTH_ICLR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51077">
        <property type="entry name" value="HTH_ICLR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51078">
        <property type="entry name" value="ICLR_ED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH iclR-type" evidence="3">
        <location>
            <begin position="16"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="domain" description="IclR-ED" evidence="4">
        <location>
            <begin position="79"/>
            <end position="258"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABF07223.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="261" mass="27528" checksum="FFE11D95AF7E19BB" modified="2006-05-30" version="1">MARTPGEGSGKTQRGIQSVEVGGRLLQALADARRPLPLAELAAAAQLAPAQAHTYLVSLMRLGLIKREHVDGYYEPGPLALRLGMMRLDNEPAYRAAVQRVRTLATDIGFNVAISLPTPQGPTIVHYAPAGTSLHLNLHVGTVMALATTATGRAYCAFQPESHWLPIWTQQQTPATASTLPAFLETLAAVRARGTERSIDSPSPAVSSLAMPLLDDDSTLRLVLTAIGSTGAIDVAWDGPVASALRAAIGDIARAMREGDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="100" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LRK7</accession>
    <name>Q1LRK7_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Regulatory protein, ArsR CMGI-7</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">arsR</name>
        <name evidence="2" type="ordered locus">Rmet_0333</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07219.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515214.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0333"/>
    <dbReference type="EnsemblBacteria" id="ABF07219">
        <property type="protein sequence ID" value="ABF07219"/>
        <property type="gene ID" value="Rmet_0333"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0333"/>
    <dbReference type="eggNOG" id="COG0640">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_097806_2_2_4"/>
    <dbReference type="OMA" id="CDCPTGD"/>
    <dbReference type="OrthoDB" id="1800563at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00090">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011991">
        <property type="entry name" value="ArsR-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001845">
        <property type="entry name" value="HTH_ArsR_DNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00778">
        <property type="entry name" value="HTHARSR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00418">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50987">
        <property type="entry name" value="HTH_ARSR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH arsR-type" evidence="1">
        <location>
            <begin position="1"/>
            <end position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF07219.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="109" mass="11549" checksum="ECFA322D1C2A242D" modified="2006-05-30" version="1">METENALEALAALAHGIRLAVFRLLVQAGPEGLPAGRIAELMEMPASSLSFHLKELHRAGLLASRQEGRSIIYMAQFETMNALLGYLTENCCGGAPCSPVSSCSVATES</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="104" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LRR8</accession>
    <name>Q1LRR8_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">RNA polymerase sigma factor RpoH</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">RNA polymerase sigma-32 factor</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6 9" type="primary">rpoH</name>
        <name evidence="9" type="ordered locus">Rmet_0272</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is involved in regulation of expression of heat shock genes.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Interacts with the RNA polymerase core enzyme.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the sigma-70 factor family. RpoH subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07158.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008642691.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0272"/>
    <dbReference type="EnsemblBacteria" id="ABF07158">
        <property type="protein sequence ID" value="ABF07158"/>
        <property type="gene ID" value="Rmet_0272"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0272"/>
    <dbReference type="eggNOG" id="COG0568">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014793_3_5_4"/>
    <dbReference type="OMA" id="YQRWLAE"/>
    <dbReference type="OrthoDB" id="1565734at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016987">
        <property type="term" value="F:sigma factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006352">
        <property type="term" value="P:DNA-templated transcription, initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009408">
        <property type="term" value="P:response to heat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00961">
        <property type="entry name" value="Sigma70_RpoH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014284">
        <property type="entry name" value="RNA_pol_sigma-70_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000943">
        <property type="entry name" value="RNA_pol_sigma70"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009042">
        <property type="entry name" value="RNA_pol_sigma70_r1_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007627">
        <property type="entry name" value="RNA_pol_sigma70_r2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007630">
        <property type="entry name" value="RNA_pol_sigma70_r4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013325">
        <property type="entry name" value="RNA_pol_sigma_r2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013324">
        <property type="entry name" value="RNA_pol_sigma_r3/r4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012759">
        <property type="entry name" value="RNA_pol_sigma_RpoH_proteobac"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00140">
        <property type="entry name" value="Sigma70_r1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04542">
        <property type="entry name" value="Sigma70_r2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04545">
        <property type="entry name" value="Sigma70_r4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00046">
        <property type="entry name" value="SIGMA70FCT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88659">
        <property type="entry name" value="SSF88659"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88946">
        <property type="entry name" value="SSF88946"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02392">
        <property type="entry name" value="rpoH_proteo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02937">
        <property type="entry name" value="sigma70-ECF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00715">
        <property type="entry name" value="SIGMA70_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00716">
        <property type="entry name" value="SIGMA70_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0731">Sigma factor</keyword>
    <keyword evidence="2 6" id="KW-0346">Stress response</keyword>
    <keyword evidence="5 6" id="KW-0804">Transcription</keyword>
    <keyword evidence="1 6" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="RNA_pol_sigma70" evidence="7">
        <location>
            <begin position="100"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="domain" description="RNA_pol_sigma70" evidence="8">
        <location>
            <begin position="277"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="H-T-H motif" evidence="6">
        <location>
            <begin position="278"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="region of interest" description="Sigma-70 factor domain-2" evidence="6">
        <location>
            <begin position="76"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Interaction with polymerase core subunit RpoC" evidence="6">
        <location>
            <begin position="100"/>
            <end position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS00715"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS00716"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ABF07158.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="311" mass="34896" checksum="DD04E16AA42C1AF7" modified="2006-05-30" version="1">MNAVLSADQTQVTRRLPTAPQNAAANAFALTFPGTLGNLDSYIQAVHRIPMLTPEEELSLARDLRDNDSVDAARRMVMSHLRLVVSIARQYLGYGLPHADLIQEGNIGLMKAVKRFDPEQGVRLVSYAMHWIKAEIHEYVLKNWRMVKVATTKAQRKLFFNLRSHKQGSHTFTPEQIEAVARELNVKPEEVMEMETRMSGGDMALEGQIEDGEEEFAPIAYLADNHNEPTQVLEAKRHDRLQVEGLEEALTKLDPRSRRIIEARWLDVNDDGSGGATLHELADEFGVSAERIRQIESAAMKKMKGALAAFA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LS27</accession>
    <name>Q1LS27_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Thiamine biosynthesis oxidoreductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">thiO</name>
        <name evidence="4" type="ordered locus">Rmet_0163</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the DAMOX/DASOX family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07049.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515075.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0163"/>
    <dbReference type="EnsemblBacteria" id="ABF07049">
        <property type="protein sequence ID" value="ABF07049"/>
        <property type="gene ID" value="Rmet_0163"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0163"/>
    <dbReference type="eggNOG" id="COG0665">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007884_1_0_4"/>
    <dbReference type="OMA" id="CRRLEPM"/>
    <dbReference type="OrthoDB" id="912110at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003884">
        <property type="term" value="F:D-amino-acid oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046416">
        <property type="term" value="P:D-amino acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023209">
        <property type="entry name" value="DAO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006076">
        <property type="entry name" value="FAD-dep_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11530">
        <property type="entry name" value="PTHR11530"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01266">
        <property type="entry name" value="DAO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="SSF51905"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DAO" evidence="3">
        <location>
            <begin position="15"/>
            <end position="357"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF07049.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="410" mass="43522" checksum="CF560EDBFCD9E794" modified="2006-05-30" version="1">MPPANNLMTAVQSFDVAILGAGLAGRLSAWQLVRAGARVALIERGGPDGSGSAAHVAAAMLAPLAESAIAERRIVDLGMTSVDLWRTWIGELPEPVFFQEDGTLVVWHARDRSEVSLFTGRMRAVAPPDLVQQRLRALDGEGVGQIEPALAGRFPQGLLLQGEGQLDNRGAMHALLNCAVTEGLHCVWEAGDIDADALPALGIRAGVVLDCRGLGARAAWTNASAGGETALPGLRGLRGEVVRVHAPDVKLHRPVRLLHPRYPIYIAPKPNDLYVIGATELESEDESPMSVRSALELLSAAHSLHPAFGEARVLELNVQRRPTRPDHLPAIRVDQQARVVRVNGLYRHGWLIAPAVTEAACAVVRAMLGGDPAAHAVPAALRWPGMIEAVQERGTAMLSARAGAGSGLLH</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LS44</accession>
    <name>Q1LS44_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Muropeptide transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">ampG</name>
        <name evidence="7" type="ordered locus">Rmet_0146</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07032.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515060.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0146"/>
    <dbReference type="EnsemblBacteria" id="ABF07032">
        <property type="protein sequence ID" value="ABF07032"/>
        <property type="gene ID" value="Rmet_0146"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0146"/>
    <dbReference type="eggNOG" id="COG2223">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029352_1_0_4"/>
    <dbReference type="OMA" id="ANPFYVD"/>
    <dbReference type="OrthoDB" id="1165704at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004752">
        <property type="entry name" value="AmpG_permease/AT-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12778">
        <property type="entry name" value="PTHR12778"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="SSF103473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="21"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="52"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="83"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="107"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="150"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="174"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="226"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="263"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="292"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="316"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="355"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="383"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="domain" description="MFS" evidence="6">
        <location>
            <begin position="14"/>
            <end position="407"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABF07032.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="426" mass="45885" checksum="AE46EC5B81DA7E1A" modified="2006-05-30" version="1">MTFQDYLDIFRNRRIGAMLTLGFASGLPLALTSGTLQAWMTVEGLDIKTIGFFSLVGQAYIFKFLWAPLMDRFTPPLLGRRRGWLLVTQLGLVGSIAAMAFCPPHEALWALAALATLVAFLSASQDIVFDAYSTDVLRPSERGAGAAVKVLGYRLAMLVSGGLALYLADRVLGWPHMYLLMAALMSVGLVTLIWAPEPDVPARAPRSLEEAVVGPLRDFFGRQGAWWLLLLIVLYKLGDAFAGSLSTTFLIRGLGFDPGEVGIVNKTLGLAATIIGALFGGSLMVRLGLYRSLMLFGILQGVSNLGYWILSVTPPHLWTMGATIAVENLCGGMGTAAFVALLMTLCNRSFSATQYALLSALASIGRVYVGPTSGYMVEAWGWAPFYLGTVIAAVPGVALLWAMRNTVHRYEAQAREPAEPAVPRPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="57" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LSC2</accession>
    <name>Q1LSC2_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Predicted integral membrane protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Rmet_0068</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF06954.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011514993.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0068"/>
    <dbReference type="EnsemblBacteria" id="ABF06954">
        <property type="protein sequence ID" value="ABF06954"/>
        <property type="gene ID" value="Rmet_0068"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0068"/>
    <dbReference type="eggNOG" id="COG5652">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_057832_0_0_4"/>
    <dbReference type="OMA" id="QWLGWLW"/>
    <dbReference type="OrthoDB" id="1739047at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006976">
        <property type="entry name" value="VanZ-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04892">
        <property type="entry name" value="VanZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="45"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="83"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="117"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="149"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="182"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="255"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="284"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="318"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="343"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="383"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="domain" description="VanZ" evidence="3">
        <location>
            <begin position="58"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF04892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF06954.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="440" mass="48916" checksum="9A86EA73DFD4D930" modified="2006-05-30" version="1">MAQATPPPSPHSPAPPEPQRSQPEDTRQSELEVALLPRHSPVARVGLLCFTLLVVYASLYPFTGWVNNGVSAFAFLTAPKPRYITEFDLLTNVLGYCPFGALVVLSLHPRVSGARATLIALVAGALLSGSMEALQTWLPTRISSNIDLITNALGSLLGAAVVAPFTSALVDRGTLTRLRYAWFEPYASFAIILILLWPFAQIFPQEHLFGMGGIVREWLTDPDSWPMELVQAYFPSLIAWQDNIGLRPDDMQGQLMLETMVTASSWIGTGLFASIAMRRTAPMLRILAGLLTAALIIKAAVAELQFPDDNAFNWLSEGGRFALLTSSLVLVLLLRLPRWLRAILATLALATLIVLTNVLPSNPYAWISEQGWRMGRFIHFNSLAQWLGWLWPLLAFFYIIWRFEQASVRRRLVRRARRRATERAVAAATEAQQESKRPQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LSE5</accession>
    <name>Q1LSE5_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transglutaminase-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_0045</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
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        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF06931.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011514972.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0045"/>
    <dbReference type="EnsemblBacteria" id="ABF06931">
        <property type="protein sequence ID" value="ABF06931"/>
        <property type="gene ID" value="Rmet_0045"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0045"/>
    <dbReference type="eggNOG" id="COG1305">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008973_0_0_4"/>
    <dbReference type="OMA" id="WGRDFSD"/>
    <dbReference type="OrthoDB" id="670657at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013589">
        <property type="entry name" value="Bac_transglu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002931">
        <property type="entry name" value="Transglutaminase-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08379">
        <property type="entry name" value="Bact_transglu_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01841">
        <property type="entry name" value="Transglut_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00460">
        <property type="entry name" value="TGc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="SSF54001"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TGc" evidence="1">
        <location>
            <begin position="183"/>
            <end position="253"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF06931.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="300" mass="32840" checksum="7561DC4145CC339B" modified="2006-05-30" version="1">MSDIGTTTLSVHHTTTYHYAWPVELAQQRAVVTPPSCAWQTVGAHSIEVDPAPSHQHMRTDGFGNNVLHFMLDVPHETMWLTATSTVTLKPRWQALDIARSPRWEDAAERLRYRAGQPFHPETEFVYASPNVALHDSLRNYALLSFAPGVTVAAGAIDLMHRIHADFDYHSEITEVHTPALEAFALRSGVCQDFAQVMIGCLRSIGLPARYVSGYLCTDPPPGEPRLLGADASHAWAGVWCPENGWIDLDPTNAMLADTRHVTLAIARDYSDVPLLHGVIVGGGEHTVGVEVSVVPVMSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-04-26" modified="2021-06-02" version="68" xmlns="http://uniprot.org/uniprot">
    <accession>Q58AP2</accession>
    <name>Q58AP2_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_6003</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="1 2">
        <name>pMOL30</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid pMOL30</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000354">
        <property type="protein sequence ID" value="ABF12862.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011229327.1">
        <property type="nucleotide sequence ID" value="NC_007971.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_145573.1">
        <property type="nucleotide sequence ID" value="NC_006466.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF12862">
        <property type="protein sequence ID" value="ABF12862"/>
        <property type="gene ID" value="Rmet_6003"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6003"/>
    <dbReference type="HOGENOM" id="CLU_1523940_0_0_4"/>
    <dbReference type="OrthoDB" id="1455181at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid pMOL30"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF12862.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="176" mass="20262" checksum="31C156B92F5E2FBF" modified="2005-04-26" version="1">MNEALPTKHELRRQMHEALGSWLYARRATSKTKQERAEHVLDYRRRRNQLAAGMYREEVNARGNTVHRDLDADLALGFVSRDRYVYDSKLCGAGWQQYDTDQDAPYFGCWVNLEKRWTMCYAEGDRILVKCPSEESLKAELADMDRFYGRAPAAFTLFASGGQATQLVCERPSVTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-04-26" modified="2022-01-19" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>Q58AT4</accession>
    <name>Q58AT4_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">ATPase involved in plasmid partitioning ParA</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">parA</name>
        <name evidence="2" type="ordered locus">Rmet_6063</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2 3">
        <name>pMOL30</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid pMOL30</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000354">
        <property type="protein sequence ID" value="ABF12922.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011229285.1">
        <property type="nucleotide sequence ID" value="NC_007971.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_145531.1">
        <property type="nucleotide sequence ID" value="NC_006466.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF12922">
        <property type="protein sequence ID" value="ABF12922"/>
        <property type="gene ID" value="Rmet_6063"/>
    </dbReference>
    <dbReference type="GeneID" id="60825054"/>
    <dbReference type="KEGG" id="rme:Rmet_6063"/>
    <dbReference type="HOGENOM" id="CLU_741596_0_0_4"/>
    <dbReference type="OMA" id="FLICPIR"/>
    <dbReference type="OrthoDB" id="729012at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid pMOL30"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025669">
        <property type="entry name" value="AAA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13614">
        <property type="entry name" value="AAA_31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA_31" evidence="1">
        <location>
            <begin position="87"/>
            <end position="284"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF12922.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="374" mass="41759" checksum="879F9B08993BD421" modified="2005-04-26" version="1">MTATEVEIDPYPYTDFASLAYPPQFAAEALGVSPQTLKIIERDNDLKISRVPRGSVEVRNYSLNDIFQIARIRRESKHIKGFTRPITISVFVPKGGTAKTTTTCNLAIQFSLMGLKVLVIDNDQQADVSTMMGYDPDLTAEELIDMGIPGERSINGHIGNLMRVGNFYQPMSLEEVVKKPFGEHGPHLIPAEDSLDDMDSALRSANGSDFRYSLFFEQARTGALPHCNLSSYDVIIMDNAPAGSLLSRNSMVAADFLICPIRMDKFSFRALSRLAFRLGEFAKDFKRAPEIVAIPTMYVRNRPRVERNMASLISLFPGKVTESRLYHSEDYSKSLEDGVPLSLWRTGTENSLGAMREVFEELVARIRAVTEVAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-01-04" modified="2022-05-25" version="124" xmlns="http://uniprot.org/uniprot">
    <accession>Q5NV32</accession>
    <name>Q5NV32_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 4">Single-stranded DNA-binding protein</fullName>
            <shortName evidence="2">SSB</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">traC2</name>
        <name evidence="6" type="ordered locus">Rmet_6278</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="6 7">
        <name>pMOL28</name>
    </geneLocation>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="7">Plasmid pMOL28</plasmid>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="2">Homotetramer.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CP000355">
        <property type="protein sequence ID" value="ABF13137.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011239918.1">
        <property type="nucleotide sequence ID" value="NC_007972.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_161658.1">
        <property type="nucleotide sequence ID" value="NC_006525.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF13137">
        <property type="protein sequence ID" value="ABF13137"/>
        <property type="gene ID" value="Rmet_6278"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6278"/>
    <dbReference type="HOGENOM" id="CLU_078758_0_1_4"/>
    <dbReference type="OMA" id="RIRSWYK"/>
    <dbReference type="OrthoDB" id="1942216at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid pMOL28"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04496">
        <property type="entry name" value="SSB_OBF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00984">
        <property type="entry name" value="SSB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000424">
        <property type="entry name" value="Primosome_PriB/ssb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011344">
        <property type="entry name" value="ssDNA-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10302">
        <property type="entry name" value="PTHR10302"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00436">
        <property type="entry name" value="SSB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00621">
        <property type="entry name" value="ssb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50935">
        <property type="entry name" value="SSB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 2 3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0614">Plasmid</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="110"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="121"/>
            <end position="138"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00252"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000524"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABF13137.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="190" mass="20882" checksum="5F8B9AFF655CD29A" modified="2005-01-04" version="1">MASVNKVILVGNLGADPETRYLPSGDAVTNIRIATTDRYKDKQSNEFKEATEWHRIAFFGKLAEIAGQYLRKGSSVYIEGRIRTRKWQDQSGQDKYSTEIVADQMQMLGGRQGGQSADDDASEGAGRRASEDQLHGQRHQQRSTSGGASARNAQQVGYGGGGAQRDGFDGFDEEIPFHAVRALDGIPFRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-01-04" modified="2022-08-03" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>Q5NV45</accession>
    <name>Q5NV45_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Conjugal transfer protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">trbE</name>
        <name evidence="3" type="ordered locus">Rmet_6305</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3 4">
        <name>pMOL28</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid pMOL28</plasmid>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TrbE/VirB4 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000355">
        <property type="protein sequence ID" value="ABF13164.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008649457.1">
        <property type="nucleotide sequence ID" value="NC_007972.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_161636.1">
        <property type="nucleotide sequence ID" value="NC_006525.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF13164">
        <property type="protein sequence ID" value="ABF13164"/>
        <property type="gene ID" value="Rmet_6305"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6305"/>
    <dbReference type="HOGENOM" id="CLU_008341_1_0_4"/>
    <dbReference type="OMA" id="HRDLNTL"/>
    <dbReference type="OrthoDB" id="182908at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid pMOL28"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018145">
        <property type="entry name" value="CagE_TrbE_VirB_cntrl_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03135">
        <property type="entry name" value="CagE_TrbE_VirB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CagE_TrbE_VirB" evidence="2">
        <location>
            <begin position="298"/>
            <end position="407"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF03135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF13164.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="818" mass="92547" checksum="89EF10EC53A57D51" modified="2005-01-04" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2010-03-23" modified="2020-02-26" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>D3DXN7</accession>
    <name>D3DXN7_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Putative mRNA endoribonuclease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">yjgH</name>
        <name evidence="1" type="ordered locus">Rmet_6443</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ADC45057.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_6443"/>
    <dbReference type="EnsemblBacteria" id="ADC45057">
        <property type="protein sequence ID" value="ADC45057"/>
        <property type="gene ID" value="Rmet_6443"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6443"/>
    <dbReference type="HOGENOM" id="CLU_2938420_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADC45057.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="60" mass="6738" checksum="D395D4D1730BC515" modified="2010-03-23" version="1">MHAAAHPTYARLWDISSTHLDPINSLIHLMQASIDLVQPPRESLIHLTNRIGKPPVHLVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-03-23" modified="2022-01-19" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>D3DXY2</accession>
    <name>D3DXY2_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Putative cyclase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_6545</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ADC45152.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADC45152">
        <property type="protein sequence ID" value="ADC45152"/>
        <property type="gene ID" value="Rmet_6545"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6545"/>
    <dbReference type="HOGENOM" id="CLU_1884064_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004061">
        <property type="term" value="F:arylformamidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019441">
        <property type="term" value="P:tryptophan catabolic process to kynurenine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.30.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007325">
        <property type="entry name" value="KFase/CYL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037175">
        <property type="entry name" value="KFase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31118">
        <property type="entry name" value="PTHR31118"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04199">
        <property type="entry name" value="Cyclase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102198">
        <property type="entry name" value="SSF102198"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADC45152.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="135" mass="14511" checksum="5219970DFB08B1A0" modified="2010-03-23" version="1">MGPCRVIHCIGAAPRVEPQHIAHALAGTPPRVLLRTYAQAPQGKWDSAFCAVAPETISLLARHGVRLIGIDTPSLDPETSKTMDAHHAVRDHQLAILEGIVLDEVPAGDYELIALPLRLATLDASPVRAVLRELP</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-03-23" modified="2021-06-02" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>D3DYC3</accession>
    <name>D3DYC3_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_6713</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ADC45293.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519584.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_6713"/>
    <dbReference type="EnsemblBacteria" id="ADC45293">
        <property type="protein sequence ID" value="ADC45293"/>
        <property type="gene ID" value="Rmet_6713"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6713"/>
    <dbReference type="HOGENOM" id="CLU_2823894_0_0_4"/>
    <dbReference type="OrthoDB" id="2102075at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADC45293.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="74" mass="8705" checksum="27F41F927B5FB4D6" modified="2010-03-23" version="1">MLNLNTRTLFESLMYKDRHLKFDVVERTYGDWCWTYTIDGRTTFSSHGNSYPTRELAAIDAMDEALLRIDKNPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-03-23" modified="2021-06-02" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>D3DYG1</accession>
    <name>D3DYG1_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_6752</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ADC45331.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008643166.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_6752"/>
    <dbReference type="EnsemblBacteria" id="ADC45331">
        <property type="protein sequence ID" value="ADC45331"/>
        <property type="gene ID" value="Rmet_6752"/>
    </dbReference>
    <dbReference type="GeneID" id="60824431"/>
    <dbReference type="KEGG" id="rme:Rmet_6752"/>
    <dbReference type="HOGENOM" id="CLU_2635513_0_0_4"/>
    <dbReference type="OrthoDB" id="1949572at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADC45331.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="77" mass="8646" checksum="71F7714B3D69A7D4" modified="2010-03-23" version="1">MRVTIRTTTIPGAPDRAALHRAAVYLNTEEDASPLMVSAWSQREPEAFLAAQRWARNHAYQVSNPRNGTYYGGRTAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="54" xmlns="http://uniprot.org/uniprot">
    <accession>Q1L9X2</accession>
    <name>Q1L9X2_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Lipoprotein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">chrY</name>
        <name evidence="2" type="ordered locus">Rmet_6195</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2 3">
        <name>pMOL28</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid pMOL28</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000355">
        <property type="protein sequence ID" value="ABF13054.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011514874.1">
        <property type="nucleotide sequence ID" value="NC_007972.2"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF13054">
        <property type="protein sequence ID" value="ABF13054"/>
        <property type="gene ID" value="Rmet_6195"/>
    </dbReference>
    <dbReference type="GeneID" id="60825788"/>
    <dbReference type="KEGG" id="rme:Rmet_6195"/>
    <dbReference type="HOGENOM" id="CLU_045679_1_0_4"/>
    <dbReference type="OMA" id="MGVNTWA"/>
    <dbReference type="OrthoDB" id="987169at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid pMOL28"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011094">
        <property type="entry name" value="Uncharacterised_LppY/LpqO"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07485">
        <property type="entry name" value="DUF1529"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193240" evidence="1">
        <location>
            <begin position="24"/>
            <end position="308"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF13054.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="308" mass="32820" checksum="9A92ADB628DC8157" modified="2006-05-30" version="1">MQAAKQVVLAVSCIIGACTIATAAPVERRVALDTTRLEQITGLKGSFSEAENVFKVSKPRTDVPIKVDGAAMPPFMGLTSWAAFTPAHGHQVMLMGDTVLLEDEVNPAMSVALEAGLDVTALHNHFFFDQPRVYFMHIGGMGNPDQLAAGVKKLYDKIAEIRAANPSVGSGFSGAIPHENAISAAPLETVFGKKGQASNGMFKVVIGAKGTMHGVTFGNEMGLNTWAAFAGTDDSAIVDGDFAMREEELQVVLKAMRSGGINIVAIHQHMTNEQPRYMFLHYWGKGKATELAASIKKALDAQAGVHKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-10-07" version="52" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LA84</accession>
    <name>Q1LA84_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_6083</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="1 2">
        <name>pMOL30</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid pMOL30</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000354">
        <property type="protein sequence ID" value="ABF12942.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF12942">
        <property type="protein sequence ID" value="ABF12942"/>
        <property type="gene ID" value="Rmet_6083"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6083"/>
    <dbReference type="HOGENOM" id="CLU_1033941_0_0_4"/>
    <dbReference type="OMA" id="FHGCRPL"/>
    <dbReference type="OrthoDB" id="1411929at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid pMOL30"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF12942.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="269" mass="30725" checksum="8F0F9BC9A90E9954" modified="2006-05-30" version="1">MNTKVFDWRMRRNWLASIRHYAGHVLTVELRQHLSQERPKCWSDDFAWLPDSEYVLPEFVAGLTSYYTHFKGFHGCRPLKLASYYERGLLGQDSELLCDAFREIFSDVPKEDVDSAIKQFADRSDRERGKMWLVGSDEFLVKQCGHYLIQGSEYLMALAAHLGRPLSGEDYRFRLRNYGIPTILEVDVPVGLVPAAQHVEVAKMILSEWGQLAARRPLGFGGEPPCYVVRQDIPPDCVQGHYHPKQIVDPHQGNRPYINGLLTCDVCGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="105" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LAG2</accession>
    <name>Q1LAG2_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Heat shock protein DnaJ-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">dnaJ</name>
        <name evidence="4" type="ordered locus">Rmet_6005</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4 5">
        <name>pMOL30</name>
    </geneLocation>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="5">Plasmid pMOL30</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000354">
        <property type="protein sequence ID" value="ABF12864.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011514811.1">
        <property type="nucleotide sequence ID" value="NC_007971.2"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF12864">
        <property type="protein sequence ID" value="ABF12864"/>
        <property type="gene ID" value="Rmet_6005"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6005"/>
    <dbReference type="HOGENOM" id="CLU_411529_0_0_4"/>
    <dbReference type="OrthoDB" id="1738789at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid pMOL30"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002939">
        <property type="entry name" value="DnaJ_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008971">
        <property type="entry name" value="HSP40/DnaJ_pept-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036410">
        <property type="entry name" value="HSP_DnaJ_Cys-rich_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022550">
        <property type="entry name" value="NTP_transf_8"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01556">
        <property type="entry name" value="DnaJ_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12281">
        <property type="entry name" value="NTP_transf_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00625">
        <property type="entry name" value="JDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="SSF46565"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49493">
        <property type="entry name" value="SSF49493"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57938">
        <property type="entry name" value="SSF57938"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0143">Chaperone</keyword>
    <keyword evidence="4" id="KW-0614">Plasmid</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0346">Stress response</keyword>
    <feature type="domain" description="J" evidence="3">
        <location>
            <begin position="4"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="78"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="331"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="347"/>
            <end position="365"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF12864.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="645" mass="70136" checksum="50EF4B2B78A61040" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LAH8</accession>
    <name>Q1LAH8_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Cytochrome c, mono-and diheme variants</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Rmet_5989</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="6 7">
        <name>pMOL30</name>
    </geneLocation>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="7">Plasmid pMOL30</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000354">
        <property type="protein sequence ID" value="ABF12848.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF12848">
        <property type="protein sequence ID" value="ABF12848"/>
        <property type="gene ID" value="Rmet_5989"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5989"/>
    <dbReference type="HOGENOM" id="CLU_383431_0_0_4"/>
    <dbReference type="OrthoDB" id="1297285at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid pMOL30"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.760.10">
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009056">
        <property type="entry name" value="Cyt_c-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036909">
        <property type="entry name" value="Cyt_c-like_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00034">
        <property type="entry name" value="Cytochrom_C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13442">
        <property type="entry name" value="Cytochrome_CBB3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46626">
        <property type="entry name" value="SSF46626"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51007">
        <property type="entry name" value="CYTC"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 4" id="KW-0349">Heme</keyword>
    <keyword evidence="3 4" id="KW-0408">Iron</keyword>
    <keyword evidence="2 4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0614">Plasmid</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cytochrome c" evidence="5">
        <location>
            <begin position="106"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="domain" description="Cytochrome c" evidence="5">
        <location>
            <begin position="252"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="domain" description="Cytochrome c" evidence="5">
        <location>
            <begin position="396"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="domain" description="Cytochrome c" evidence="5">
        <location>
            <begin position="658"/>
            <end position="751"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABF12848.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="773" mass="82834" checksum="A392F39E67A6287C" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="58" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LAI0</accession>
    <name>Q1LAI0_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Negative regulation of plasmid gene expression transcriptional repressor activity</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">bimB2</name>
        <name evidence="4" type="ordered locus">Rmet_5987</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4 5">
        <name>pMOL30</name>
    </geneLocation>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="5">Plasmid pMOL30</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000354">
        <property type="protein sequence ID" value="ABF12846.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011514817.1">
        <property type="nucleotide sequence ID" value="NC_007971.2"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF12846">
        <property type="protein sequence ID" value="ABF12846"/>
        <property type="gene ID" value="Rmet_5987"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5987"/>
    <dbReference type="HOGENOM" id="CLU_876819_0_0_4"/>
    <dbReference type="OMA" id="AAQHRYE"/>
    <dbReference type="OrthoDB" id="1283285at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid pMOL30"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021104">
        <property type="entry name" value="KfrA_DNA-bd_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11740">
        <property type="entry name" value="KfrA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0614">Plasmid</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="KfrA_N" evidence="3">
        <location>
            <begin position="7"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="287"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="98"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="215"/>
            <end position="256"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF11740"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF12846.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="312" mass="33457" checksum="1B9B59BE57C7B57D" modified="2006-05-30" version="1">MPRLAATPDQIRATVLAMLTEAGDAAPPTAARFRRVVSVRKLRDRLGGGDPATLSRTLNAIEAEVVRAGLADLALPELPAEIAEAMRALWQAAVAVQLDDVVRLRREAQQTAEAAQAARAEAELRVELLREELSEVRAQLAARDTALAEASAALAAASARADAQAARRGELETALATVQERVMTDERAHAEAIATVQARYEALSKQLLQETAHQRDAVRAERAQLASQLKFAERRIAALEEERGRLDAELASERAARQTAAGEASALKAVTASQRAQLDELMRATLAVAPPPSKTRVPRSTDKPAGKRTVKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LAP8</accession>
    <name>Q1LAP8_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">PNPLA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Rmet_5919</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="6">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="6">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12778.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5919"/>
    <dbReference type="EnsemblBacteria" id="ABF12778">
        <property type="protein sequence ID" value="ABF12778"/>
        <property type="gene ID" value="Rmet_5919"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5919"/>
    <dbReference type="eggNOG" id="COG1752">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG4775">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014750_1_0_4"/>
    <dbReference type="OMA" id="DFEHVNY"/>
    <dbReference type="OrthoDB" id="373926at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019867">
        <property type="term" value="C:outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016035">
        <property type="entry name" value="Acyl_Trfase/lysoPLipase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000184">
        <property type="entry name" value="Bac_surfAg_D15"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002641">
        <property type="entry name" value="PNPLA_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01103">
        <property type="entry name" value="Omp85"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01734">
        <property type="entry name" value="Patatin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52151">
        <property type="entry name" value="SSF52151"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51635">
        <property type="entry name" value="PNPLA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="1 2" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="5" id="KW-0614">Plasmid</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193016" description="PNPLA domain-containing protein" evidence="3">
        <location>
            <begin position="34"/>
            <end position="750"/>
        </location>
    </feature>
    <feature type="domain" description="PNPLA" evidence="4">
        <location>
            <begin position="50"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="GXGXXG" evidence="2">
        <location>
            <begin position="54"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="GXSXG" evidence="2">
        <location>
            <begin position="81"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="DGA/G" evidence="2">
        <location>
            <begin position="228"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="2">
        <location>
            <position position="83"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="2">
        <location>
            <position position="228"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABF12778.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="750" mass="80344" checksum="E0CF7B5D43D7F406" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="65" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LAR0</accession>
    <name>Q1LAR0_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_5907</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12766.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5907"/>
    <dbReference type="EnsemblBacteria" id="ABF12766">
        <property type="protein sequence ID" value="ABF12766"/>
        <property type="gene ID" value="Rmet_5907"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5907"/>
    <dbReference type="HOGENOM" id="CLU_152565_0_0_4"/>
    <dbReference type="OMA" id="CKRIEHD"/>
    <dbReference type="OrthoDB" id="1836244at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193265" evidence="1">
        <location>
            <begin position="24"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF12766.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="107" mass="12366" checksum="0FD667285A07434B" modified="2006-05-30" version="1">MPVLRYLLILLPQLLLMPLIAQAEPDLVQCQRVDQSQLRSASEETLLFLRCRAREISYEAPRLKGVTQKFKDDLVFSCLDQADAVEHQLRVGYGYTRETLAKKKCDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="72" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LAS1</accession>
    <name>Q1LAS1_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">L-lactate permease</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">Rmet_5896</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="10">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="10">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Transports L-lactate across the membrane. Can also transport D-lactate and glycolate.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cell inner membrane</location>
            <topology evidence="8">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the lactate permease family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12755.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008646178.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5896"/>
    <dbReference type="EnsemblBacteria" id="ABF12755">
        <property type="protein sequence ID" value="ABF12755"/>
        <property type="gene ID" value="Rmet_5896"/>
    </dbReference>
    <dbReference type="GeneID" id="60823693"/>
    <dbReference type="KEGG" id="rme:Rmet_5896"/>
    <dbReference type="eggNOG" id="COG1620">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021628_0_0_4"/>
    <dbReference type="OMA" id="VIVLWIQ"/>
    <dbReference type="OrthoDB" id="732251at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005887">
        <property type="term" value="C:integral component of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015129">
        <property type="term" value="F:lactate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003804">
        <property type="entry name" value="Lactate_perm"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30003">
        <property type="entry name" value="PTHR30003"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02652">
        <property type="entry name" value="Lactate_perm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00795">
        <property type="entry name" value="lctP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0614">Plasmid</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 8" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="41"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="72"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="116"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="155"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="199"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="223"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="252"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="282"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="314"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="382"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="422"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="445"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="543"/>
            <end position="561"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010100"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU365092"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ABF12755.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="566" mass="58665" checksum="87DB374EBEAAF8E4" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-10-07" version="66" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LAS3</accession>
    <name>Q1LAS3_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Extra-cytoplasmic solute receptor protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">bug</name>
        <name evidence="3" type="ordered locus">Rmet_5894</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Periplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the UPF0065 (bug) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12753.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5894"/>
    <dbReference type="EnsemblBacteria" id="ABF12753">
        <property type="protein sequence ID" value="ABF12753"/>
        <property type="gene ID" value="Rmet_5894"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5894"/>
    <dbReference type="eggNOG" id="COG3181">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045683_0_0_4"/>
    <dbReference type="OMA" id="FAPVIQI"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042597">
        <property type="term" value="C:periplasmic space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005064">
        <property type="entry name" value="BUG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042100">
        <property type="entry name" value="Bug_dom1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42928">
        <property type="entry name" value="PTHR42928"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03401">
        <property type="entry name" value="TctC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017082">
        <property type="entry name" value="YflP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-0675">Receptor</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF12753.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="358" mass="37878" checksum="4C1EBF837D719A0A" modified="2006-05-30" version="1">MRPQRRTSRARGAHSKHKKLYRRQHIMPQWHRRAALAALTSLVACTFTLTTTPAFAQKEFPSKPIMLVVTYPPGGPTDAMARTLAAALKNSLGQPVVVENRAGAGGNIGAEVVARAEPDGYTLMFGTSAPLAINVSLYRKINYDPVKSFAPVIQIGQLPNALVVNPSVPAKNVQELIAYSKAHPGKLSYASSGNGASSHLAGVLFNNLAGTDFQHVPYKGTGPALNDLLGGQVSMTFTDVLTALPFIKSGKVRALGVTTKGRSQALPDVPTVAEQGVAGFDVSVFFGVVAPAGTPPEVIRKLNHAFADALAQPDVRKTLLAQGLEFAPATTPEQLGGFVKSEVGKWRSVVQKSGAQLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="63" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LAU3</accession>
    <name>Q1LAU3_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_5874</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12733.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518083.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5874"/>
    <dbReference type="EnsemblBacteria" id="ABF12733">
        <property type="protein sequence ID" value="ABF12733"/>
        <property type="gene ID" value="Rmet_5874"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5874"/>
    <dbReference type="HOGENOM" id="CLU_061786_0_0_4"/>
    <dbReference type="OrthoDB" id="1932860at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193250" evidence="1">
        <location>
            <begin position="36"/>
            <end position="379"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF12733.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="379" mass="36735" checksum="82CD9ED943DD6B55" modified="2006-05-30" version="1">MVQTTISPAPRCTARSTTAAVLLGAGLACAGNAHAGVETLAPESADTAGVATPVAIDLPAMPTPTQLSALSEDPITAAARAADPIVADNVAENVSGKAVESIAEAPARGVEPVDKASVARHHPIGDTLVAAMTMATREPAAQVAEARDGMARWKPVAQARLEESRGGFDVAGLQVGFGIDRAVFVNGALAVATSITIPNISSITSAQAAQLAQALAPVTVAVTGANSIASAAIAAAQAATGQAGQAGQSATTAANGAAASAQGAAGVAASTSTAAASGGIAQATGAAGVAQQQAATAVAAAGTVVTNGLLTVIQNGAANTANVGSVANMPATVIQNTLNNQNIQSLMTINASVNTLAAFRAQMANTALNSVIPTMAGMK</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="103" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LAW8</accession>
    <name>Q1LAW8_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="20">Tyrosine-protein kinase</fullName>
            <ecNumber evidence="20">2.7.10.2</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="20" type="primary">epsB</name>
        <name evidence="20" type="ordered locus">Rmet_5849</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="21">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="21" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="21">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + L-tyrosyl-[protein] = ADP + H(+) + O-phospho-L-tyrosyl-[protein]</text>
            <dbReference type="Rhea" id="RHEA:10596"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:10137"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:82620"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell inner membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the etk/wzc family.</text>
    </comment>
    <dbReference type="EC" id="2.7.10.2" evidence="20"/>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12708.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518057.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5849"/>
    <dbReference type="EnsemblBacteria" id="ABF12708">
        <property type="protein sequence ID" value="ABF12708"/>
        <property type="gene ID" value="Rmet_5849"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5849"/>
    <dbReference type="eggNOG" id="COG0489">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG3206">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009912_0_0_4"/>
    <dbReference type="OMA" id="ETQYALM"/>
    <dbReference type="OrthoDB" id="1184929at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004715">
        <property type="term" value="F:non-membrane spanning protein tyrosine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045226">
        <property type="term" value="P:extracellular polysaccharide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009103">
        <property type="term" value="P:lipopolysaccharide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025669">
        <property type="entry name" value="AAA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005700">
        <property type="entry name" value="EPS_ExoP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005702">
        <property type="entry name" value="EPS_synthesis"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032807">
        <property type="entry name" value="GNVR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003856">
        <property type="entry name" value="LPS_length_determ_N_term"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13614">
        <property type="entry name" value="AAA_31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13807">
        <property type="entry name" value="GNVR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02706">
        <property type="entry name" value="Wzz"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01007">
        <property type="entry name" value="eps_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01005">
        <property type="entry name" value="eps_transp_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="15" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="10 20" id="KW-0418">Kinase</keyword>
    <keyword evidence="13 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="20" id="KW-0614">Plasmid</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 20" id="KW-0808">Transferase</keyword>
    <keyword evidence="8 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12 16" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="14" id="KW-0829">Tyrosine-protein kinase</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="34"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="domain" description="Wzz" evidence="17">
        <location>
            <begin position="21"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="domain" description="GNVR" evidence="19">
        <location>
            <begin position="393"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="domain" description="AAA_31" evidence="18">
        <location>
            <begin position="560"/>
            <end position="671"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="15">
        <location>
            <begin position="272"/>
            <end position="322"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001074"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004429"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF02706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF13614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF13807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="ABF12708.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="748" mass="81617" checksum="1AD3E75154A1083A" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="54" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LB03</accession>
    <name>Q1LB03_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_5814</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12673.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF12673">
        <property type="protein sequence ID" value="ABF12673"/>
        <property type="gene ID" value="Rmet_5814"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5814"/>
    <dbReference type="HOGENOM" id="CLU_142131_0_0_4"/>
    <dbReference type="OMA" id="EWILSQQ"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="141"/>
            <end position="161"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF12673.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="161" mass="18043" checksum="8F474CB9FB226B16" modified="2006-05-30" version="1">MLCNGEQIMTAGEIRRPAIGPISESAKPSAHRAWTVLGEASAGIALSDWHEDYAIAEVAAVLADDQEVSPRTRAAVEGLLHRHRLLAEELSRQRKLLQEWILSQQTYKTLYFQYSGQSPDAPNIELMLEKDMVRRRIRDQLSAGRDQVSVAEESPHSLSRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LB15</accession>
    <name>Q1LB15_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Phosphogluconate dehydratase</fullName>
            <ecNumber evidence="6">4.2.1.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">edd1</name>
        <name evidence="6" type="synonym">edd</name>
        <name evidence="8" type="ordered locus">Rmet_5802</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="9">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="9">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Catalyzes the dehydration of 6-phospho-D-gluconate to 2-dehydro-3-deoxy-6-phospho-D-gluconate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>6-phospho-D-gluconate = 2-dehydro-3-deoxy-6-phospho-D-gluconate + H2O</text>
            <dbReference type="Rhea" id="RHEA:17277"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:57569"/>
            <dbReference type="ChEBI" id="CHEBI:58759"/>
            <dbReference type="EC" id="4.2.1.12"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="6">Binds 1 [4Fe-4S] cluster.</text>
    </comment>
    <comment type="pathway">
        <text evidence="6">Carbohydrate metabolism; Entner-Doudoroff pathway.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the IlvD/Edd family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.12" evidence="6"/>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12661.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5802"/>
    <dbReference type="EnsemblBacteria" id="ABF12661">
        <property type="protein sequence ID" value="ABF12661"/>
        <property type="gene ID" value="Rmet_5802"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5802"/>
    <dbReference type="eggNOG" id="COG0129">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014271_1_2_4"/>
    <dbReference type="OMA" id="KADERPN"/>
    <dbReference type="UniPathway" id="UPA00226"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004456">
        <property type="term" value="F:phosphogluconate dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046177">
        <property type="term" value="P:D-gluconate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009255">
        <property type="term" value="P:Entner-Doudoroff pathway through 6-phosphogluconate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.30.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02094">
        <property type="entry name" value="Edd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004786">
        <property type="entry name" value="6-phosphgluc_deHydtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042096">
        <property type="entry name" value="Dihydro-acid_dehy_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000581">
        <property type="entry name" value="DiOHA_6PGluconate_deHydtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020558">
        <property type="entry name" value="DiOHA_6PGluconate_deHydtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037237">
        <property type="entry name" value="IlvD/EDD_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43661:SF1">
        <property type="entry name" value="PTHR43661:SF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00920">
        <property type="entry name" value="ILVD_EDD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143975">
        <property type="entry name" value="SSF143975"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01196">
        <property type="entry name" value="edd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00886">
        <property type="entry name" value="ILVD_EDD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00887">
        <property type="entry name" value="ILVD_EDD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 6" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="5 6" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="3 6" id="KW-0311">Gluconate utilization</keyword>
    <keyword evidence="6" id="KW-0408">Iron</keyword>
    <keyword evidence="6" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="4 6 8" id="KW-0456">Lyase</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0614">Plasmid</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="248"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="315"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006486"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ABF12661.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="700" mass="74961" checksum="F82BF704EB294AD7" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-10-07" version="65" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LB79</accession>
    <name>Q1LB79_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_5738</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12597.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5738"/>
    <dbReference type="EnsemblBacteria" id="ABF12597">
        <property type="protein sequence ID" value="ABF12597"/>
        <property type="gene ID" value="Rmet_5738"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5738"/>
    <dbReference type="eggNOG" id="ENOG5032WPE">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1348004_0_0_4"/>
    <dbReference type="OMA" id="SCARISR"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF12597.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="203" mass="22116" checksum="0171DD3D7AF7546A" modified="2006-05-30" version="1">MLNLSSQVERVSCARISRVAFILPVFATLATGCATSSVSAAEISMEQNTELTSEVSAKPVTTDSDADEEARSFQKQLWINPGFISLHFTKIEERNPLNLGFGAEWRFNEDTGVIAGTYVNSVRKQSTYGGVSWQPITLGATQLGAALGVIRGYPKLNHGGWIPMILPTLSYAYGRFGFNLVLVPPLGDKNVGSLSLQLKFKVW</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="66" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LBH9</accession>
    <name>Q1LBH9_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_5638</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12497.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011520041.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5638"/>
    <dbReference type="EnsemblBacteria" id="ABF12497">
        <property type="protein sequence ID" value="ABF12497"/>
        <property type="gene ID" value="Rmet_5638"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5638"/>
    <dbReference type="eggNOG" id="COG5266">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_079897_0_0_4"/>
    <dbReference type="OMA" id="PEAGMYW"/>
    <dbReference type="OrthoDB" id="1382281at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019613">
        <property type="entry name" value="DUF4198"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10670">
        <property type="entry name" value="DUF4198"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193296" evidence="1">
        <location>
            <begin position="31"/>
            <end position="277"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF12497.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="277" mass="29865" checksum="15216CB1D0351189" modified="2006-05-30" version="1">MNKPARFSTRLSTRVAALALAALAPLAAHAHRQWLLPSTTVLSGGSQWVTVDAAVSNDLFYFEHMPMRLDNLVVTGPDGAAVKPENASTGKYRSTFDVPLAQPGTYRIAVVNQGLVASWKENGQTKRWRGAADALAANVPANAQELQVTEMASRVESYVTSGKPSKQALTTTGRGLELAPITHPNDLAAGETASFRLLLDGKPAANQTVSVVPGGVRYRDRLNEFDTTTDADGKFSVKWPAAGMYWMEAELRDQKTSVKPATARRVTYAVTVEVLPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LCP6</accession>
    <name>Q1LCP6_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Extra-cytoplasmic solute receptor Bug family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">bug</name>
        <name evidence="3" type="ordered locus">Rmet_5221</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Periplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the UPF0065 (bug) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12080.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5221"/>
    <dbReference type="EnsemblBacteria" id="ABF12080">
        <property type="protein sequence ID" value="ABF12080"/>
        <property type="gene ID" value="Rmet_5221"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5221"/>
    <dbReference type="eggNOG" id="COG3181">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045683_0_0_4"/>
    <dbReference type="OMA" id="CAPAIAD"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042597">
        <property type="term" value="C:periplasmic space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005064">
        <property type="entry name" value="BUG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042100">
        <property type="entry name" value="Bug_dom1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006311">
        <property type="entry name" value="TAT_signal"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42928">
        <property type="entry name" value="PTHR42928"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03401">
        <property type="entry name" value="TctC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017082">
        <property type="entry name" value="YflP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51318">
        <property type="entry name" value="TAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-0675">Receptor</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF12080.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="347" mass="37172" checksum="F833B09141B9FD75" modified="2006-05-30" version="1">MLADLRLLNLRTTLTRTPAMSINRRTFLKATALGSLGGLELPAFAADKYPTRPITLVVPFSPGGSVDVSGRLIAERLGRAIGQPVVIDNRPGAGGAIGSTYVAKAQPDGYTLIVTSQSTHVVNPAMNPKLPYDAVKGFSNITLISRLSNVLLCNASLPIRNFAELVKYARANPTALNYASAGTGSVSHVSMELLKAQAGIPMTHVPYRGAGTALTDLLGGSVQLTWNNLSSNLDHILQGKLRALAVAAPARVPQLPDVPTFAELKLPDLNETSWTGLAAPAGTPHAIVQQLYLAVRKILAEPSTRSIWTAKGMIVPEDITPEAYTRETEQRIKFYQRIAKDNHITLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="72" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LCR4</accession>
    <name>Q1LCR4_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Putative 2-aminomuconate deaminase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_5203</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12062.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519608.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5203"/>
    <dbReference type="EnsemblBacteria" id="ABF12062">
        <property type="protein sequence ID" value="ABF12062"/>
        <property type="gene ID" value="Rmet_5203"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5203"/>
    <dbReference type="eggNOG" id="COG0251">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_100715_7_3_4"/>
    <dbReference type="OMA" id="EITATAW"/>
    <dbReference type="OrthoDB" id="1850770at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035959">
        <property type="entry name" value="RutC-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006175">
        <property type="entry name" value="YjgF/YER057c/UK114"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11803">
        <property type="entry name" value="PTHR11803"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01042">
        <property type="entry name" value="Ribonuc_L-PSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55298">
        <property type="entry name" value="SSF55298"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF12062.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="147" mass="15490" checksum="FB99E147EA585FE7" modified="2006-05-30" version="1">MNPPPLAAYSPARRAGGHVHLAGMSPRTHDGCAGVSTGANGERVYDLAAQTECVLDKIDRALQAEGLELVNCVTMTCYLVDMRDYAAFNAAYDAICMRRFSSAGQPLPARTCVAVAALPHPDMRVEITATAWGPDTPDSPSNARGAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LCW7</accession>
    <name>Q1LCW7_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Enoyl-CoA hydratase</fullName>
            <ecNumber evidence="3">4.2.1.17</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_5150</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the enoyl-CoA hydratase/isomerase family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.17" evidence="3"/>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12009.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008645293.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5150"/>
    <dbReference type="EnsemblBacteria" id="ABF12009">
        <property type="protein sequence ID" value="ABF12009"/>
        <property type="gene ID" value="Rmet_5150"/>
    </dbReference>
    <dbReference type="GeneID" id="60824763"/>
    <dbReference type="KEGG" id="rme:Rmet_5150"/>
    <dbReference type="eggNOG" id="COG1024">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009834_7_2_4"/>
    <dbReference type="OMA" id="NPPHAVR"/>
    <dbReference type="OrthoDB" id="1275789at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004300">
        <property type="term" value="F:enoyl-CoA hydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.12.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029045">
        <property type="entry name" value="ClpP/crotonase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001753">
        <property type="entry name" value="Enoyl-CoA_hydra/iso"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014748">
        <property type="entry name" value="Enoyl-CoA_hydra_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00378">
        <property type="entry name" value="ECH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52096">
        <property type="entry name" value="SSF52096"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0456">Lyase</keyword>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF12009.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="264" mass="28290" checksum="45127FF66A4B22D2" modified="2006-05-30" version="1">MSNFLSYEQDGHVVTLTMSEPERRNPLTGNTAVPEFLAAIERIESDTSVRAVIITGSGTAFSSGGNIHEMKRQASGTASAMDVRRDYRHGIQRLPLALFNLEVPVIAAVNGPALGAGLDLTCMCDIRIASRAAQFAESFVKLGIIPGDGGAWLLPRIIGMSRAAELTFTGKAIDAQTALEWNLVSQVVEPEALLPTAREIAAAIAANPPHAVRLAKRLLREGMHTSLGTLLELSAVYQAVSHQAADHKEAVEAFLDKRKPVFKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LD31</accession>
    <name>Q1LD31_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">FAD dependent oxidoreductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_5086</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11945.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519492.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5086"/>
    <dbReference type="EnsemblBacteria" id="ABF11945">
        <property type="protein sequence ID" value="ABF11945"/>
        <property type="gene ID" value="Rmet_5086"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5086"/>
    <dbReference type="eggNOG" id="COG0665">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007884_4_2_4"/>
    <dbReference type="OMA" id="FMESYGT"/>
    <dbReference type="OrthoDB" id="912110at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006076">
        <property type="entry name" value="FAD-dep_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01266">
        <property type="entry name" value="DAO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="SSF51905"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="domain" description="DAO" evidence="2">
        <location>
            <begin position="11"/>
            <end position="353"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF11945.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="385" mass="40678" checksum="D7F5A6FF76FE7241" modified="2006-05-30" version="1">MTATTAPLRSDFLVIGAGIAGASVAYWLAPHGSVTVLERESQPGYHSTGRSAALFMESYGTPQVRALTMASHAFLANPPQGFATHPLLTPRGALMVAAPGQQALLDEHWEILRAMAPEAELLTAQQACERVPVLRPEMVIGAVYEPGAADMDVHAIHQGYLRGIRQAGGKLVCDAEVRAIARHGDVWRVEAGQAVYEAPVVLNAAGAWADVIARQAGVSPLGLQPCRRSAFVFQPPAGVDSHGWPMVHSADESWYIKPDAGMLLGSPANADPVEPQDVQPEELDIAMAIHRIEEATTLTIRHPARTWAGLRSFVADGDLVGGFDDRAPGFFWVAAQGGYGIQTSAAMGETCAALARGLPVPAHAASFGLTAEMLGPARLRRAAAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LDL6</accession>
    <name>Q1LDL6_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Putative patatin-like phospholipase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Rmet_4898</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="6">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="6">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11760.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519320.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4898"/>
    <dbReference type="EnsemblBacteria" id="ABF11760">
        <property type="protein sequence ID" value="ABF11760"/>
        <property type="gene ID" value="Rmet_4898"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4898"/>
    <dbReference type="eggNOG" id="COG1752">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047251_4_0_4"/>
    <dbReference type="OMA" id="PFRCCAA"/>
    <dbReference type="OrthoDB" id="373926at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016035">
        <property type="entry name" value="Acyl_Trfase/lysoPLipase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002641">
        <property type="entry name" value="PNPLA_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01734">
        <property type="entry name" value="Patatin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52151">
        <property type="entry name" value="SSF52151"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51635">
        <property type="entry name" value="PNPLA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="1 2" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="5" id="KW-0614">Plasmid</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PNPLA" evidence="4">
        <location>
            <begin position="6"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="274"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="GXGXXG" evidence="2">
        <location>
            <begin position="10"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="GXSXG" evidence="2">
        <location>
            <begin position="37"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="DGA/G" evidence="2">
        <location>
            <begin position="163"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="2">
        <location>
            <position position="39"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="2">
        <location>
            <position position="163"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABF11760.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="313" mass="33182" checksum="896A03506BFC7D92" modified="2006-05-30" version="1">MEKTAFVFAGGGSLGAIEVGMLRELVKWGVAPDLVIGASAGAINGAYFACNPHAEGTARLESLWRSITRAEVLPWSWRSMFGMIAGNRGHLVESFGLRRLLNRNFGAARLEESAVPLYVVATDMQTGAEVVLSAGNIVDAVLASAAIPGVFPPVEVGGRKLIDGGVANNTPVSTAIRLGATRIIVLPAGFACAERRPPRGAVEHAFNALSLLVARQLVYDLERWSEQAQIAVVPPLCPLDISPYDYTRCGELIERAAEATVAWLRADGLSSRRVPGALEPHTHDEASPSCSTDLLGPTSGHAHAKQHGHGHPH</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LDY8</accession>
    <name>Q1LDY8_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative N-acetyltransferase, GNAT family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_4776</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11638.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519207.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4776"/>
    <dbReference type="EnsemblBacteria" id="ABF11638">
        <property type="protein sequence ID" value="ABF11638"/>
        <property type="gene ID" value="Rmet_4776"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4776"/>
    <dbReference type="eggNOG" id="COG0456">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013985_35_0_4"/>
    <dbReference type="OMA" id="YIMNMYT"/>
    <dbReference type="OrthoDB" id="2055010at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008080">
        <property type="term" value="F:N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="SSF55729"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="1">
        <location>
            <begin position="1"/>
            <end position="155"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF11638.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="155" mass="17372" checksum="256932D4AC990315" modified="2006-05-30" version="1">MDVRTIGVDDLDLICQHREAMFQEAGFAPGTLRTMTDHFRAWLRPRLADGSYFGFLLTDAGRPVAGIGLMLVDWPPHPAHPTQDRRGYVLNVYVAPTRRRRGLAQQLMALAEAEFARRGVEFAVLHATEAGRPLYQRSGWNQTSEMSKVIAPPAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="60" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LEL6</accession>
    <name>Q1LEL6_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_4545</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11410.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519001.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF11410">
        <property type="protein sequence ID" value="ABF11410"/>
        <property type="gene ID" value="Rmet_4545"/>
    </dbReference>
    <dbReference type="GeneID" id="60825978"/>
    <dbReference type="KEGG" id="rme:Rmet_4545"/>
    <dbReference type="HOGENOM" id="CLU_1990854_0_0_4"/>
    <dbReference type="OMA" id="ACETPPQ"/>
    <dbReference type="OrthoDB" id="1952643at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193393" evidence="2">
        <location>
            <begin position="23"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="22"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="88"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="22"/>
            <end position="36"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF11410.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="137" mass="14870" checksum="CE0D66F059EEB67C" modified="2006-05-30" version="1">MPRLSYPCLALAALLAACTSTPMPPPTPPQRTPPTSVAPPAGNYGGVSRAQMEAAGNACEKMVANAVKNRYPQPGSVMMMPDRERIYQQSASQAGVSGEGTFEPDGTEESMSFRYTCLFNLRTNHVDDVQMRYGPYY</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="67" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LEM8</accession>
    <name>Q1LEM8_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_4533</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11398.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518991.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4533"/>
    <dbReference type="EnsemblBacteria" id="ABF11398">
        <property type="protein sequence ID" value="ABF11398"/>
        <property type="gene ID" value="Rmet_4533"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4533"/>
    <dbReference type="eggNOG" id="COG2304">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_113195_1_0_4"/>
    <dbReference type="OMA" id="WPRAQVA"/>
    <dbReference type="OrthoDB" id="2065585at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025489">
        <property type="entry name" value="DUF4381"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14316">
        <property type="entry name" value="DUF4381"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="38"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF11398.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="165" mass="18175" checksum="7B60BA377D89D37C" modified="2006-05-30" version="1">MNELIDIVTPTPPSWMPQTVGWAMVGVVLLAGVLWLAWRTARRWYANRFRRAALRELTALQQTADSAEQATLLLSLAELLKRLALVQWPRHAVAALSGPAWRDFLHTHAGGAGDAVPALAALIDDAEYRGAATLAQWPEAQLRATLGACRIWIAGYRHDGHDDHV</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LEW0</accession>
    <name>Q1LEW0_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Universal stress protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">uspA10</name>
        <name evidence="4" type="ordered locus">Rmet_4451</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="5">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="5">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the universal stress protein A family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11316.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518920.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4451"/>
    <dbReference type="EnsemblBacteria" id="ABF11316">
        <property type="protein sequence ID" value="ABF11316"/>
        <property type="gene ID" value="Rmet_4451"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4451"/>
    <dbReference type="eggNOG" id="COG0589">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_049301_11_0_4"/>
    <dbReference type="OMA" id="AMCVVSD"/>
    <dbReference type="OrthoDB" id="2040681at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006015">
        <property type="entry name" value="Universal_stress_UspA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006016">
        <property type="entry name" value="UspA"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00582">
        <property type="entry name" value="Usp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006276">
        <property type="entry name" value="UspA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01438">
        <property type="entry name" value="UNVRSLSTRESS"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0614">Plasmid</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Usp" evidence="3">
        <location>
            <begin position="1"/>
            <end position="146"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008791"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRNR" id="PIRNR006276"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF11316.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="161" mass="17396" checksum="4CD6A07265A2B135" modified="2006-05-30" version="1">MHQHILVAVDDSPCARRALNEAVGLASAFHAKLEILHVIDYSFLQYDFGYGDFTELRPQLTAAGESLLKEAGEIAGAAGLEHHETLIDDLATMGNIADRVNGYARRCSADIVVIGTHGRHGLKRMLLGSVAESLARDCPVPVVLVRDDSPARTAERPVAQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-10-07" version="70" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LEW2</accession>
    <name>Q1LEW2_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Extra-cytoplasmic solute receptor (Bug)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">bug</name>
        <name evidence="4" type="ordered locus">Rmet_4449</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="5">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="5">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Periplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the UPF0065 (bug) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11314.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4449"/>
    <dbReference type="EnsemblBacteria" id="ABF11314">
        <property type="protein sequence ID" value="ABF11314"/>
        <property type="gene ID" value="Rmet_4449"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4449"/>
    <dbReference type="eggNOG" id="COG3181">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045683_0_2_4"/>
    <dbReference type="OMA" id="FEINTWN"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042597">
        <property type="term" value="C:periplasmic space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005064">
        <property type="entry name" value="BUG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042100">
        <property type="entry name" value="Bug_dom1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42928">
        <property type="entry name" value="PTHR42928"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03401">
        <property type="entry name" value="TctC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017082">
        <property type="entry name" value="YflP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-0675">Receptor</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193087" evidence="3">
        <location>
            <begin position="27"/>
            <end position="327"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF11314.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="327" mass="33832" checksum="5562AFA8C8C736CD" modified="2006-05-30" version="1">MNKRPILALAAAMIVGGLGTAQNAAAQYPQQPVKIVVGYAAGGTTDILARALAEQLAAELKQSVIIENRPGAAGNAAAAYVQQSAPDGYTLFMATVSSHGINPALYKKSLGYDPVNGFAPISMVASIPLVLITTPNLPVKSVPDLVALARKKPGELNYASSGNGSPVHLAGAMFAQGAGAKLVHVPYRGGALANTSVIAGETQMSFATLPGALPQVKAGRLRAIAVTTRDRSAQLPDVPAMREVPGYGNFEINTWNALLAPRNTPQPIIDSLNKAIGKAMASPKLVQRFNDEGATPVASSPADLGRFIQAELAKWAGVVGELNVKVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LEW7</accession>
    <name>Q1LEW7_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Extra-cytoplasmic solute receptor (Bug)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">bug</name>
        <name evidence="4" type="ordered locus">Rmet_4444</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="5">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="5">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Periplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the UPF0065 (bug) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11309.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518915.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4444"/>
    <dbReference type="EnsemblBacteria" id="ABF11309">
        <property type="protein sequence ID" value="ABF11309"/>
        <property type="gene ID" value="Rmet_4444"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4444"/>
    <dbReference type="eggNOG" id="COG3181">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045683_0_0_4"/>
    <dbReference type="OMA" id="AEMPHVV"/>
    <dbReference type="OrthoDB" id="447734at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042597">
        <property type="term" value="C:periplasmic space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005064">
        <property type="entry name" value="BUG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042100">
        <property type="entry name" value="Bug_dom1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42928">
        <property type="entry name" value="PTHR42928"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03401">
        <property type="entry name" value="TctC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017082">
        <property type="entry name" value="YflP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-0675">Receptor</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193131" evidence="3">
        <location>
            <begin position="26"/>
            <end position="323"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF11309.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="323" mass="33704" checksum="607E25971DAF1933" modified="2006-05-30" version="1">MPRFSRCLPLIAALLGTVVPAIAHAAYPDHALRLIVPFSAGGSSDLQARMLADKLGKLYGQPVVVENRPGAGGHIGGKAVADAAPDGYTLLLGSIGLHATYGTYPKLNYDPSKDLKVVTVLAEMPHVVLVNPSIQVTNLKQLVDAAAKRPDGMTFGSAGVGSSVHMIGELFKVTTGAHLTHVPYKGSSAAIADLLGGQIDMMFENPPTTLGYIRSGKLRALAVTGKTRLPALPQVPTAAEAGVPNFVATSWTTVAVSAKVPDAIADKLNADIRSIVASQEFRSALAEQGMTPVGNTRDAATTFIAGEKARWDQVIAKARLTAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LEY2</accession>
    <name>Q1LEY2_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Citrate lyase beta subunit-like protein</fullName>
            <ecNumber evidence="7">4.1.3.34</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">citE</name>
        <name evidence="7" type="ordered locus">Rmet_4429</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="8">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="8">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <dbReference type="EC" id="4.1.3.34" evidence="7"/>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11294.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4429"/>
    <dbReference type="EnsemblBacteria" id="ABF11294">
        <property type="protein sequence ID" value="ABF11294"/>
        <property type="gene ID" value="Rmet_4429"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4429"/>
    <dbReference type="eggNOG" id="COG2301">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_073837_0_0_4"/>
    <dbReference type="OMA" id="FVSGHHG"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008816">
        <property type="term" value="F:citryl-CoA lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005000">
        <property type="entry name" value="Aldolase/citrate-lyase_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040186">
        <property type="entry name" value="Citramalyl-CoA_lyase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011206">
        <property type="entry name" value="Citrate_lyase_beta/mcl1/mcl2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015813">
        <property type="entry name" value="Pyrv/PenolPyrv_Kinase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040442">
        <property type="entry name" value="Pyrv_Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11105">
        <property type="entry name" value="PTHR11105"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03328">
        <property type="entry name" value="HpcH_HpaI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015582">
        <property type="entry name" value="Cit_lyase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51621">
        <property type="entry name" value="SSF51621"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0456">Lyase</keyword>
    <keyword evidence="4" id="KW-0460">Magnesium</keyword>
    <keyword evidence="2 4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0614">Plasmid</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HpcH_HpaI" evidence="6">
        <location>
            <begin position="94"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="29"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="129"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3 4">
        <location>
            <position position="194"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="220"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR015582-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR015582-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF03328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABF11294.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="373" mass="40671" checksum="FDC7A6CCF08BDD31" modified="2006-05-30" version="1">MPGRQCRGGNDKAVTTRSGVVPGPSDPSRTRHTTHDTHERQSMTTATHPRDALFAGEKAFPALAACEHFAGSEKLIGKAMDLQAEYGPVFDVTCDCEDGAATGQEREHAEMVARMIASDRNRFGRAGARIHDPSHPAWRQDVEIIVGQAGGRLAYITVPKATRHEQVAEVIAFIRETAQRAGLERPVPVHVLIETHGALRDVFPIAELPDIEVLDFGLMDFVSGHHGAIPAAAMRSPGQFEHALLARAKADMVAAALANGIVPAHNVCLNLKDADVIGADASRARNQFGFLRMWSIYPAQILSIVNAMRPDFAEVEDAAGILLAAQDADWGPIQYKGELHDRATYRYFWEVLEKARVTGMAMPAEAERRFFAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="63" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LF34</accession>
    <name>Q1LF34_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Phospholipid/glycerol acyltransferase</fullName>
            <ecNumber evidence="3">2.3.1.51</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">plsC</name>
        <name evidence="3" type="ordered locus">Rmet_4377</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EC" id="2.3.1.51" evidence="3"/>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11242.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518860.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4377"/>
    <dbReference type="EnsemblBacteria" id="ABF11242">
        <property type="protein sequence ID" value="ABF11242"/>
        <property type="gene ID" value="Rmet_4377"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4377"/>
    <dbReference type="eggNOG" id="COG0204">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_078753_0_0_4"/>
    <dbReference type="OMA" id="QMIIANH"/>
    <dbReference type="OrthoDB" id="1756450at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003841">
        <property type="term" value="F:1-acylglycerol-3-phosphate O-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002123">
        <property type="entry name" value="Plipid/glycerol_acylTrfase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01553">
        <property type="entry name" value="Acyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00563">
        <property type="entry name" value="PlsC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="domain" description="PlsC" evidence="2">
        <location>
            <begin position="86"/>
            <end position="195"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF11242.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="259" mass="28704" checksum="CE3D958A0C5C50BA" modified="2006-05-30" version="1">MFDRLGRAWRVVATAFCFAIFGIGGLVLGVLVFPLLSLMLWARARAQRACRRVIHSTLWGFLWLMRTLGVLDYEIRNADRLRRPGLLIVANHPSLIDAVFLLALTPDANCVVKGALARNPFTRGAVQATGWVRNDSGGVAMVDDCIASLRAGSSLIIFPEGTRTRPGAPLQLQRGAANVAVRGDIALTPVVIRCVPPTLTKGEKWYHVPRRRPRFVIDVHADVSVAQWVPQGLEPVKAVRRLNHSLSDYFSREIRREVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LF39</accession>
    <name>Q1LF39_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Transcriptional regulator, TetR/AcrR family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_4372</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11237.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4372"/>
    <dbReference type="EnsemblBacteria" id="ABF11237">
        <property type="protein sequence ID" value="ABF11237"/>
        <property type="gene ID" value="Rmet_4372"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4372"/>
    <dbReference type="eggNOG" id="COG1309">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069356_4_1_4"/>
    <dbReference type="OMA" id="VTQGRCL"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001647">
        <property type="entry name" value="HTH_TetR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036271">
        <property type="entry name" value="Tet_transcr_reg_TetR-rel_C_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00440">
        <property type="entry name" value="TetR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48498">
        <property type="entry name" value="SSF48498"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50977">
        <property type="entry name" value="HTH_TETR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH tetR-type" evidence="2">
        <location>
            <begin position="23"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="H-T-H motif" evidence="1">
        <location>
            <begin position="46"/>
            <end position="65"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF11237.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="224" mass="24868" checksum="25A8B629CE591599" modified="2006-05-30" version="1">MCGTRQVDKPTGRAILKPMQKGQLTRTAIIEQAFRSATQVGFEQLSLATLAADTNMSKSGLYAHFKSKEALQEAVLEHAMERFGEIVINPAMRQRRGAPRLLALFDGYLAWLGGTVVEGRCIFMALSQEYANRPGVIRDKVVQAFKDWHSTIVRVIGDAVDEGVLRADTDAHQFAFEMEGIGMSFQSSFKLMGRASAETMARRAFARLVNDLKENRAEPLVATR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="104" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LF71</accession>
    <name>Q1LF71_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Probable malate:quinone oxidoreductase</fullName>
            <ecNumber evidence="5">1.1.5.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">MQO</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="5">Malate dehydrogenase [quinone]</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5 7" type="primary">mqo</name>
        <name evidence="7" type="ordered locus">Rmet_4340</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="8">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="8">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 5">
            <text>(S)-malate + a quinone = a quinol + oxaloacetate</text>
            <dbReference type="Rhea" id="RHEA:46012"/>
            <dbReference type="ChEBI" id="CHEBI:15589"/>
            <dbReference type="ChEBI" id="CHEBI:16452"/>
            <dbReference type="ChEBI" id="CHEBI:24646"/>
            <dbReference type="ChEBI" id="CHEBI:132124"/>
            <dbReference type="EC" id="1.1.5.4"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 5">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3 5">Carbohydrate metabolism; tricarboxylic acid cycle; oxaloacetate from (S)-malate (quinone route): step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 5">Belongs to the MQO family.</text>
    </comment>
    <dbReference type="EC" id="1.1.5.4" evidence="5"/>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11205.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518827.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4340"/>
    <dbReference type="EnsemblBacteria" id="ABF11205">
        <property type="protein sequence ID" value="ABF11205"/>
        <property type="gene ID" value="Rmet_4340"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4340"/>
    <dbReference type="eggNOG" id="COG0579">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028151_0_0_4"/>
    <dbReference type="OMA" id="PHLDTRW"/>
    <dbReference type="OrthoDB" id="1371190at2"/>
    <dbReference type="UniPathway" id="UPA00223">
        <property type="reaction ID" value="UER01008"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052589">
        <property type="term" value="F:malate dehydrogenase (menaquinone) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008924">
        <property type="term" value="F:malate dehydrogenase (quinone) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00212">
        <property type="entry name" value="MQO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006231">
        <property type="entry name" value="MQO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006311">
        <property type="entry name" value="TAT_signal"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06039">
        <property type="entry name" value="Mqo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="SSF51905"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01320">
        <property type="entry name" value="mal_quin_oxido"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51318">
        <property type="entry name" value="TAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0274">FAD</keyword>
    <keyword evidence="5" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-0614">Plasmid</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0816">Tricarboxylic acid cycle</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="22"/>
            <end position="41"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABF11205.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="571" mass="62375" checksum="723C446B0761C010" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LF82</accession>
    <name>Q1LF82_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Transcriptional regulator, BadM/Rrf2 family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_4329</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11194.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518818.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4329"/>
    <dbReference type="EnsemblBacteria" id="ABF11194">
        <property type="protein sequence ID" value="ABF11194"/>
        <property type="gene ID" value="Rmet_4329"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4329"/>
    <dbReference type="eggNOG" id="COG1959">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_107144_4_0_4"/>
    <dbReference type="OMA" id="GCLVEQA"/>
    <dbReference type="OrthoDB" id="1794390at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000944">
        <property type="entry name" value="Tscrpt_reg_Rrf2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33221">
        <property type="entry name" value="PTHR33221"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02082">
        <property type="entry name" value="Rrf2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51197">
        <property type="entry name" value="HTH_RRF2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF11194.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="147" mass="15929" checksum="A61B7C91946FB1F6" modified="2006-05-30" version="1">MRFDTRLARLLHVLVHMHLRGGSTTSENIALMLHTNPVVVRRTMAALRDAGYVQSTGGRGGGWDLARDLEDFTVKDVYVAIAHTALFALGPADDNPACPVEAAVNRHINEALSSAEMTMLRFFGEKRLAEIARDATSSISGKPSGQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="106" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LFB0</accession>
    <name>Q1LFB0_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="13">NADH:ubiquinone oxidoreductase complex I, chain F</fullName>
            <ecNumber evidence="13">1.6.5.3</ecNumber>
            <ecNumber evidence="13">1.6.99.5</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">nuoF</name>
        <name evidence="13" type="ordered locus">Rmet_4300</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="14">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="14">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the complex I 51 kDa subunit family.</text>
    </comment>
    <dbReference type="EC" id="1.6.5.3" evidence="13"/>
    <dbReference type="EC" id="1.6.99.5" evidence="13"/>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11166.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518793.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4300"/>
    <dbReference type="EnsemblBacteria" id="ABF11166">
        <property type="protein sequence ID" value="ABF11166"/>
        <property type="gene ID" value="Rmet_4300"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4300"/>
    <dbReference type="eggNOG" id="COG1894">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014881_0_1_4"/>
    <dbReference type="OMA" id="CKQCTYC"/>
    <dbReference type="OrthoDB" id="688908at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1440.230">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11540">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011538">
        <property type="entry name" value="Nuo51_FMN-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037225">
        <property type="entry name" value="Nuo51_FMN-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019575">
        <property type="entry name" value="Nuop51_4Fe4S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037207">
        <property type="entry name" value="Nuop51_4Fe4S-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019554">
        <property type="entry name" value="Soluble_ligand-bd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01512">
        <property type="entry name" value="Complex1_51K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10589">
        <property type="entry name" value="NADH_4Fe-4S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10531">
        <property type="entry name" value="SLBB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00928">
        <property type="entry name" value="NADH_4Fe-4S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140490">
        <property type="entry name" value="SSF140490"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF142019">
        <property type="entry name" value="SSF142019"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="5" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="6" id="KW-0288">FMN</keyword>
    <keyword evidence="9" id="KW-0408">Iron</keyword>
    <keyword evidence="10" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0520">NAD</keyword>
    <keyword evidence="13" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="13" id="KW-0614">Plasmid</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-1278">Translocase</keyword>
    <keyword evidence="13" id="KW-0830">Ubiquinone</keyword>
    <feature type="domain" description="NADH_4Fe-4S" evidence="12">
        <location>
            <begin position="326"/>
            <end position="371"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007523"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM00928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ABF11166.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="414" mass="44124" checksum="0E50578DA89022AB" modified="2006-05-30" version="1">MEKLNQVLLRDDVRVGADLEAWLAIGGGEGLAKALCDPGAVIGEIEQADLRGMGGAGFATHRKWAPVAAAADGDKTIICNGNEDEPGTFKDRFLLEHTPHQVIEGALIAAAATRANHIVLYVNPHQQQAIAVIRQAVGQWQAHPRYAELERLLGAPLSLGVVPSSGLYIGGEETAVIASVEGGFPFPRRKPPFPSQQGVHGAPSIVNNVETLAHIPGILRHGAQWYRDLGIGNATGTKLYSLSGDVLRPGLYELPMGTSLESLVFEHGGGMLQGKEFKAVFTGGPSNTLLTKRDLDVALDFDSVRLRRSRLGTGAMIVVSEGTSIVRKVAEFVSFFAQGSCGQCPPCKGGSFQLMRLLNRIDTGRGVHADLAALENLCRILPGSGRCGLIDGAVTVVESSLHQFREEYEALLMA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="96" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LFC8</accession>
    <name>Q1LFC8_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Transcriptional regulator, LysR family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_4282</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the LysR transcriptional regulatory family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11148.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518774.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4282"/>
    <dbReference type="EnsemblBacteria" id="ABF11148">
        <property type="protein sequence ID" value="ABF11148"/>
        <property type="gene ID" value="Rmet_4282"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4282"/>
    <dbReference type="eggNOG" id="COG0583">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039613_2_0_4"/>
    <dbReference type="OMA" id="QVARMGT"/>
    <dbReference type="OrthoDB" id="1102730at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005119">
        <property type="entry name" value="LysR_subst-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000847">
        <property type="entry name" value="Tscrpt_reg_HTH_LysR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00126">
        <property type="entry name" value="HTH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03466">
        <property type="entry name" value="LysR_substrate"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50931">
        <property type="entry name" value="HTH_LYSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH lysR-type" evidence="2">
        <location>
            <begin position="1"/>
            <end position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF11148.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="288" mass="31928" checksum="6012A9B49C230CDF" modified="2006-05-30" version="1">MNWDDARIFVALYRERTLRAAARALDIDQATVGRRLAVLEHALGSTLFLRTSNGYELTQVGKLAIRAAEAMEQSAHDLVRHTQGMDKRLAGEVKLSTTDALAQEYVVPAIESLHAQHPDVSVVLDTTTHVLNLAKREADIAVRTVKPRNPDLLARRLASWDVGLFASPAYIRRHGEPVPGERFLGHDLVVYQPHFAKARAPSFIGEPLTDGRIAARLNTNLTLRAALKAGLGISEIPVPMGERDGLVRIWPDRTSGAPYEVWLVTHQDLRHTARVRVTIDEIVLAFGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LFJ0</accession>
    <name>Q1LFJ0_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Transcriptional regulator, LysR family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_4219</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the LysR transcriptional regulatory family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11086.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518709.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4219"/>
    <dbReference type="EnsemblBacteria" id="ABF11086">
        <property type="protein sequence ID" value="ABF11086"/>
        <property type="gene ID" value="Rmet_4219"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4219"/>
    <dbReference type="eggNOG" id="COG0583">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039613_1_4_4"/>
    <dbReference type="OMA" id="PLPISIW"/>
    <dbReference type="OrthoDB" id="860559at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005119">
        <property type="entry name" value="LysR_subst-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000847">
        <property type="entry name" value="Tscrpt_reg_HTH_LysR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00126">
        <property type="entry name" value="HTH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03466">
        <property type="entry name" value="LysR_substrate"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00039">
        <property type="entry name" value="HTHLYSR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50931">
        <property type="entry name" value="HTH_LYSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH lysR-type" evidence="2">
        <location>
            <begin position="4"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF11086.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="283" mass="30631" checksum="E91046C8EC968E98" modified="2006-05-30" version="1">MKKLDLDVMEMIVAVADAGSFARAAETLHRSPSAVSMQIKAIEDVLGKPLFTRTTRNVALTEDGRTLLDYGRRMLALREEAWSVVVRPEIRGRVTIGVPDDYASSLLPSVLRRFAAAHPRVEIRVIGLPSHALIPLLKDNTLDLACITKARGVTGDFIRHEPMVWVSARGGRPIWRERPLPIAVFAHGSAARAGAIAALQQADIQYRMSYESPSLLGLISMVEAGLAVAPLARCSVPAHLAVLGESEGLPPLAPLEVVLARSAKSARLPCDFLAEQMLADLKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LFJ9</accession>
    <name>Q1LFJ9_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Tricarballylate dehydrogenase</fullName>
            <ecNumber evidence="5">1.3.99.1</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">tcuA</name>
        <name evidence="5" type="ordered locus">Rmet_4210</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="6">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="6">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <dbReference type="EC" id="1.3.99.1" evidence="5"/>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11077.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518700.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4210"/>
    <dbReference type="EnsemblBacteria" id="ABF11077">
        <property type="protein sequence ID" value="ABF11077"/>
        <property type="gene ID" value="Rmet_4210"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4210"/>
    <dbReference type="eggNOG" id="COG1053">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011398_4_6_4"/>
    <dbReference type="OMA" id="QPGQIAY"/>
    <dbReference type="OrthoDB" id="153138at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008152">
        <property type="term" value="P:metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.700.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012831">
        <property type="entry name" value="CobZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003953">
        <property type="entry name" value="FAD-binding_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027477">
        <property type="entry name" value="Succ_DH/fumarate_Rdtase_cat_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00890">
        <property type="entry name" value="FAD_binding_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="SSF51905"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56425">
        <property type="entry name" value="SSF56425"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02485">
        <property type="entry name" value="CobZ_N-term"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="3 5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-0614">Plasmid</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD_binding_2" evidence="4">
        <location>
            <begin position="3"/>
            <end position="430"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00890"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABF11077.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="477" mass="51695" checksum="B2538340F04A6B04" modified="2006-05-30" version="1">MIDVLVIGGGNAALCAALMAREAGASVMLLEAAPREWRGGNSSHTRNLRCMHDAPQDVLVDAYPEEEYWQDLLKVTGGLTDEKLARLAIRASSSCRDWMRSHGVHFQPPLSGALHVARTNAFFMGGGKALVNAYYRSAEALGVQIRYNAPVDAIELDGSRFVAARIGAERIEARACVLAAGGFESNREWLREAWGQNERGEWPADNFLIRGTRFNMGVLLKHMIAAGADAIGDPSQSHCVAIDARAPLYDGGICTRIDCVSLGVVLNANAERFYDEGEDFWPKRYAIWGRLVAQQPQQIGYSIIDAKAIGRFMPPVFPGVKADTLPELARKLGLDEATFMRTINAYNAACRVGKFDHTVLDDCHTEGVTPSKTHWARPLDTAPFFGYALRPGITFTYLGLKVNEESAVHFDGRPSDNLFVAGEMMAGNVLGKGYTAGVGMAIGTAFGRIAGTQAAKAAKGMARRASQASTEKEYATS</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="72" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LFL9</accession>
    <name>Q1LFL9_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative metalloproteinase (Zinc carboxypeptidase-related protein)</fullName>
            <ecNumber evidence="2">3.4.17.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_4190</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EC" id="3.4.17.-" evidence="2"/>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11057.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518679.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4190"/>
    <dbReference type="EnsemblBacteria" id="ABF11057">
        <property type="protein sequence ID" value="ABF11057"/>
        <property type="gene ID" value="Rmet_4190"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4190"/>
    <dbReference type="eggNOG" id="COG2866">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_830613_0_0_4"/>
    <dbReference type="OMA" id="DCHSGFG"/>
    <dbReference type="OrthoDB" id="397741at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004181">
        <property type="term" value="F:metallocarboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000834">
        <property type="entry name" value="Peptidase_M14"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00246">
        <property type="entry name" value="Peptidase_M14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00132">
        <property type="entry name" value="CARBOXYPEPT_ZN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0121">Carboxypeptidase</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase_M14" evidence="1">
        <location>
            <begin position="56"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS00132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF11057.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="343" mass="38830" checksum="B6849333DF3AD4C9" modified="2006-05-30" version="1">MTDTGRSSFPEYDQLLALLELGAPWFRSKIACDVTVQGHSFAVHVASLGTDAPDAPAIGIFGGIHGLERIGTQLVLDYMRSLLCRLAWDDLLHRQLQTVRLIFMPIVNPGGMYAATRANPNGVDLMRNAPQDADAAVPFLAGGQRIGAWLPWYRGRAGAPMEAESRAMLHVVETELLPRPLSFAVDCHSGYGWRDSIWFPYARTHAPMSHFPEMYLLKTMFEQAHPHHGYAFEPQSHQYLLHGDLWDFAYDRAPPQNLFLPMTLELGSWLWIKKNPRQIFSRAGIFNPIKAHRTARVLRRHVSLFDFLGRVAVAPDRWLPNGAQRDHLLEQARAHWQYGPPMP</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="67" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LFM0</accession>
    <name>Q1LFM0_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative hydrolase, alpha/beta fold family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_4189</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11056.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518678.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4189"/>
    <dbReference type="EnsemblBacteria" id="ABF11056">
        <property type="protein sequence ID" value="ABF11056"/>
        <property type="gene ID" value="Rmet_4189"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4189"/>
    <dbReference type="eggNOG" id="COG2267">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_075806_0_0_4"/>
    <dbReference type="OMA" id="HDLPHDD"/>
    <dbReference type="OrthoDB" id="1196738at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022742">
        <property type="entry name" value="Hydrolase_4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12146">
        <property type="entry name" value="Hydrolase_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="SSF53474"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Hydrolase_4" evidence="1">
        <location>
            <begin position="52"/>
            <end position="236"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF11056.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="258" mass="28256" checksum="02F4CD1482D8230F" modified="2006-05-30" version="1">MSAWIFLRGLTRESRHWGNLPALWTSRGLGQPLLIDLPGNGTAHDATVPASVDGMMEAVRAAARASGVPGPWRVLAMSLGAMVATRWVQAWPDDVAGLVLINTSMRPFSAITERLQPLNWPALLGMANHWQDRVRCERTVHRLTCERLDTRDADIAGWVEIAQSAPVSRKAAWLQLLAAARFRAMTAAPACPTLILSSDADRLVNPACSAHLAAAWRSDGVSLIRHPWAGHDLPHDDPRWLCDTVAAWKQDKKIPPDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="110" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LFP0</accession>
    <name>Q1LFP0_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">4-hydroxy-3-methylbut-2-enyl diphosphate reductase</fullName>
            <shortName evidence="5">HMBPP reductase</shortName>
            <ecNumber evidence="5">1.17.7.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5 7" type="primary">ispH</name>
        <name evidence="7" type="ordered locus">Rmet_4169</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="8">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="8">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Catalyzes the conversion of 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate (HMBPP) into a mixture of isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP). Acts in the terminal step of the DOXP/MEP pathway for isoprenoid precursor biosynthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>H2O + isopentenyl diphosphate + 2 oxidized [2Fe-2S]-[ferredoxin] = (2E)-4-hydroxy-3-methylbut-2-enyl diphosphate + 2 H(+) + 2 reduced [2Fe-2S]-[ferredoxin]</text>
            <dbReference type="Rhea" id="RHEA:24488"/>
            <dbReference type="Rhea" id="RHEA-COMP:10000"/>
            <dbReference type="Rhea" id="RHEA-COMP:10001"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33737"/>
            <dbReference type="ChEBI" id="CHEBI:33738"/>
            <dbReference type="ChEBI" id="CHEBI:128753"/>
            <dbReference type="ChEBI" id="CHEBI:128769"/>
            <dbReference type="EC" id="1.17.7.4"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>dimethylallyl diphosphate + H2O + 2 oxidized [2Fe-2S]-[ferredoxin] = (2E)-4-hydroxy-3-methylbut-2-enyl diphosphate + 2 H(+) + 2 reduced [2Fe-2S]-[ferredoxin]</text>
            <dbReference type="Rhea" id="RHEA:24825"/>
            <dbReference type="Rhea" id="RHEA-COMP:10000"/>
            <dbReference type="Rhea" id="RHEA-COMP:10001"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33737"/>
            <dbReference type="ChEBI" id="CHEBI:33738"/>
            <dbReference type="ChEBI" id="CHEBI:57623"/>
            <dbReference type="ChEBI" id="CHEBI:128753"/>
            <dbReference type="EC" id="1.17.7.4"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="5">Binds 1 [4Fe-4S] cluster per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="5">Isoprenoid biosynthesis; dimethylallyl diphosphate biosynthesis; dimethylallyl diphosphate from (2E)-4-hydroxy-3-methylbutenyl diphosphate: step 1/1.</text>
    </comment>
    <comment type="pathway">
        <text evidence="5">Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 6/6.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the IspH family.</text>
    </comment>
    <dbReference type="EC" id="1.17.7.4" evidence="5"/>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11036.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518657.1">
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        <property type="reaction ID" value="UER00105"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="lytB_ispH"/>
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    </dbReference>
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    </dbReference>
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            <end position="224"/>
        </location>
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            <end position="332"/>
        </location>
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            <end position="324"/>
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        </location>
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        <location>
            <position position="12"/>
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        <ligand>
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        <source>
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        <source>
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    </evidence>
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        <source>
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    </evidence>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="ABF11036.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="332" mass="35423" checksum="799992F00B78EB46" modified="2006-05-30" version="1">MQVILAQPRGFCAGVVRAIEIVDRALVKHGAPVYVRHEIVHNKHVVESLRQKGARFVEELDEVPGNAVTIFSAHGVSRDVVSEAARRELHAIDATCPLVIKVHNQGRQYAAAGRHVVLIGHAGHPEVEGTMGQIPGKVILVQNEADVATLDLPNDAPVAYITQTTLSVDDTRNIIAALGARFTNLVGPSTRDICYATQNRQSAVRELSTRVDVILVIGATNSSNSNRLREIGTESGVPSYLIADGSELDPTWVRDAAVVGITAGASAPEEMVEDVIAALRRLGPVEVSTMEGIEEHAEFRLPAELDNVKQAPAASSTTEPSDARLADQTAAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-10-07" version="51" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LFX9</accession>
    <name>Q1LFX9_CUPMC</name>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name evidence="2" type="ordered locus">Rmet_4080</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
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            <taxon>Betaproteobacteria</taxon>
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            <taxon>Burkholderiaceae</taxon>
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        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
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    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
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        </source>
    </reference>
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    </dbReference>
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        <location>
            <begin position="62"/>
            <end position="83"/>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF10947.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="127" mass="13595" checksum="60D5130233BDD28F" modified="2006-05-30" version="1">MAFASPQMGDGLGLIAVGQLDCAGVVAVAGVAETFGVLAAAESQAGRVSCCRCRASARPRSITAKSGPAMADSEARREAAPHRASRADFIRKLRRVGKTMPRMIRSFRHSVQDRIVIRAIGWANSWR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="72" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LG88</accession>
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        <submittedName>
            <fullName evidence="3">Putative chemotaxis signal transduction protein (CheW)</fullName>
        </submittedName>
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        <name evidence="3" type="ordered locus">Rmet_3970</name>
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        </lineage>
    </organism>
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    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
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                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
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        <property type="gene ID" value="Rmet_3970"/>
    </dbReference>
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    </dbReference>
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    <dbReference type="OMA" id="IDDCWNR"/>
    <dbReference type="OrthoDB" id="1711729at2"/>
    <dbReference type="Proteomes" id="UP000002429">
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    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="project" value="InterPro"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="CheW-like_dom_sf"/>
    </dbReference>
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        <property type="entry name" value="CheW-lke_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22617">
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
    <dbReference type="PROSITE" id="PS50851">
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        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CheW-like" evidence="2">
        <location>
            <begin position="84"/>
            <end position="247"/>
        </location>
    </feature>
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        <location>
            <begin position="149"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="149"/>
            <end position="170"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50851"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF10838.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="250" mass="26352" checksum="798752E6E63809FE" modified="2006-05-30" version="1">MAEPNSLLPHAAPAGAIDDCWRRIGVTGDRTCPLLATHITCRNCPVYAQAALVLLDTLAIDPIVQPASDSDTPIDQSSADRLAGESYMVFRVGDEWLALPTATLGEVTAPALVHSLPHRRHAALIGVAAVRGALLTCISLALLFGESAEPTESTESTESTESTESNGRGGQDAQRAARFLILGQGRNAIALPVAEVAGVERVTQDVLLPLPATLARASARYSRALFDFNGHSVGLLDADLLRQALTRSLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-10-07" version="60" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LGD7</accession>
    <name>Q1LGD7_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_3921</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10789.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF10789">
        <property type="protein sequence ID" value="ABF10789"/>
        <property type="gene ID" value="Rmet_3921"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3921"/>
    <dbReference type="HOGENOM" id="CLU_1774293_0_0_4"/>
    <dbReference type="OMA" id="GMERTPV"/>
    <dbReference type="OrthoDB" id="1800485at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="71"/>
            <end position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF10789.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="142" mass="14701" checksum="4A205C3BE0F38194" modified="2006-05-30" version="1">MPAPPRRSPGRTGMIAAAAIALTTACANLEAGTMQRIFESWKGAPVEQAKAQWGPPQTVQAAPDGTAYVWTDEMPTAHPPGSGPRDAQLEPPPTVAYCQRKLIAGADGKISRGEWSGSACCAQTLIGQCAGLARKPSATSNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-12-02" version="68" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LGG4</accession>
    <name>Q1LGG4_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_3894</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10762.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3894"/>
    <dbReference type="EnsemblBacteria" id="ABF10762">
        <property type="protein sequence ID" value="ABF10762"/>
        <property type="gene ID" value="Rmet_3894"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3894"/>
    <dbReference type="eggNOG" id="COG2990">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_813470_0_0_4"/>
    <dbReference type="OMA" id="GSELQAN"/>
    <dbReference type="OrthoDB" id="736338at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007488">
        <property type="entry name" value="DUF535"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38785">
        <property type="entry name" value="PTHR38785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04393">
        <property type="entry name" value="DUF535"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF10762.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="341" mass="37436" checksum="72B24DCCB74895DB" modified="2006-05-30" version="1">MHEPVSYLQGLVHARAGGLVDLYPAYYLRYLASVASISAFCYPAGSRKWLTRRAKLIALSAVRAGSARSWLRAAAASPLLQRVVVARPLMLEKPFRPLGALGLGFGDRARLVVDHYVLAESALPVCVCDRIYLSGGLRWMSEDGRYELRLADSGPNPKEGELAFYWTDIETGVCLTQLSFYLRRGDHGPEIYVGGLQGPMGEQSREWIRLATKASEGLRPKDVVMEALLAFAAHVGARRVVGVSRANHVSRQRNTPREILADYEGFWAEYHGTSLADGNVDVPICQPQRDIAEIPSKKRSAWRRKQAFAEAVRATVADWLRADAVPAGNDPQLPVAAAVAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LGQ2</accession>
    <name>Q1LGQ2_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Putative acyl-CoA dehydrogenase-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Rmet_3804</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="7">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="7">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the acyl-CoA dehydrogenase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10674.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518323.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3804"/>
    <dbReference type="EnsemblBacteria" id="ABF10674">
        <property type="protein sequence ID" value="ABF10674"/>
        <property type="gene ID" value="Rmet_3804"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3804"/>
    <dbReference type="eggNOG" id="COG1960">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018204_5_2_4"/>
    <dbReference type="OMA" id="MVDMYME"/>
    <dbReference type="OrthoDB" id="760677at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016627">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-CH group of donors"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.540.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.110.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046373">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036250">
        <property type="entry name" value="AcylCo_DH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009075">
        <property type="entry name" value="AcylCo_DH/oxidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013786">
        <property type="entry name" value="AcylCoA_DH/ox_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037069">
        <property type="entry name" value="AcylCoA_DH/ox_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009100">
        <property type="entry name" value="AcylCoA_DH/oxidase_NM_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00441">
        <property type="entry name" value="Acyl-CoA_dh_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02771">
        <property type="entry name" value="Acyl-CoA_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47203">
        <property type="entry name" value="SSF47203"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56645">
        <property type="entry name" value="SSF56645"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0274">FAD</keyword>
    <keyword evidence="3" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="6" id="KW-0614">Plasmid</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Acyl-CoA_dh_N" evidence="5">
        <location>
            <begin position="6"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA_dh_1" evidence="4">
        <location>
            <begin position="218"/>
            <end position="367"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF02771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABF10674.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="369" mass="39464" checksum="2AE2E5C0D0AC0F47" modified="2006-05-30" version="1">MDFTYSEEQRMLADSLRRFVESEYSFEKRRRNAREHGSFDRGIWSSLAEMGVLGLAVPADHGGFGHGPASQVVVQRELGRALVQEPVIPSGVIATAILAHHAPAALQAEWLPAIASGDKIFTLAYLEPDSRYRPEVARTTARKAGADGYVLNGRKSVVWHGAAADTYIVSAMLDGALALFLVSRDAKGLAVTGYPTMDRLSGAGLQLADVPATLVTTNGLEALELGLDHGVAAQCAAAAGAIERLIEITAEYLGTRKQFGKPLASFQALQHRVADMLLQKELALSMAYVAAQGLDATDAAERRRKVSAAKVVTAKAARFAGQQAVQLHGGMGMTDELEVGDYFKALTMVDVLLGDTDLHLERYGEAMAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LH13</accession>
    <name>Q1LH13_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Chemotaxis protein methyltransferase</fullName>
            <ecNumber evidence="5">2.1.1.80</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">cheR</name>
        <name evidence="8" type="ordered locus">Rmet_3691</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="9">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="9">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Methylation of the membrane-bound methyl-accepting chemotaxis proteins (MCP) to form gamma-glutamyl methyl ester residues in MCP.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 5">
            <text>L-glutamyl-[protein] + S-adenosyl-L-methionine = [protein]-L-glutamate 5-O-methyl ester + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:24452"/>
            <dbReference type="Rhea" id="RHEA-COMP:10208"/>
            <dbReference type="Rhea" id="RHEA-COMP:10311"/>
            <dbReference type="ChEBI" id="CHEBI:29973"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:82795"/>
            <dbReference type="EC" id="2.1.1.80"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.1.1.80" evidence="5"/>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10563.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518212.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3691"/>
    <dbReference type="EnsemblBacteria" id="ABF10563">
        <property type="protein sequence ID" value="ABF10563"/>
        <property type="gene ID" value="Rmet_3691"/>
    </dbReference>
    <dbReference type="GeneID" id="60826246"/>
    <dbReference type="KEGG" id="rme:Rmet_3691"/>
    <dbReference type="eggNOG" id="COG1352">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025854_0_0_4"/>
    <dbReference type="OMA" id="EWQEFVN"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008983">
        <property type="term" value="F:protein-glutamate O-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.155.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026024">
        <property type="entry name" value="Chemotaxis_MeTrfase_CheR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022642">
        <property type="entry name" value="CheR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000780">
        <property type="entry name" value="CheR_MeTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022641">
        <property type="entry name" value="CheR_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036804">
        <property type="entry name" value="CheR_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01739">
        <property type="entry name" value="CheR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03705">
        <property type="entry name" value="CheR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000410">
        <property type="entry name" value="CheR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00996">
        <property type="entry name" value="CHERMTFRASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00138">
        <property type="entry name" value="MeTrc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50123">
        <property type="entry name" value="CHER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 5 8" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="8" id="KW-0614">Plasmid</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5 6" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="3 5 8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="CheR-type methyltransferase" evidence="7">
        <location>
            <begin position="30"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="region of interest" description="S-adenosyl-L-methionine binding" evidence="6">
        <location>
            <begin position="225"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="region of interest" description="S-adenosyl-L-methionine binding" evidence="6">
        <location>
            <begin position="243"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="107"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="109"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="113"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="144"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="167"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001541"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRNR" id="PIRNR000410"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR000410-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ABF10563.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="303" mass="33850" checksum="8387A1F0BE028B38" modified="2006-05-30" version="1">MTPLRSSSSAIRKPGAAMTPGAPVLAPLRDEMRDFLLTERDFEKIRALIHKRAGISLGSHKREMVYSRLARRLRTLQLADFSTYLAQLEADERSSEWEYFTNALTTNLTSFFREAHHFPLLAEHAKKIGRPYSVWCAAASTGEEPYSIAITLAEALGERAGTVLATDIDTQVLAKARSGIYTAEQVSRLSQERLKRFFLKGTGARAGSVKVKPELAATVTFEPLNLLAPDWGLREKFDAIFCRNVMIYFDKPTQGQILERFAPLLKPNGLLFAGHSENFSYVTRAFQLRGQTVYELTNKSGAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LH26</accession>
    <name>Q1LH26_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Methyl-accepting chemotaxis sensory transducer</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">Rmet_3678</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="12">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="12">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the methyl-accepting chemotaxis (MCP) protein family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10550.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518201.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3678"/>
    <dbReference type="EnsemblBacteria" id="ABF10550">
        <property type="protein sequence ID" value="ABF10550"/>
        <property type="gene ID" value="Rmet_3678"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3678"/>
    <dbReference type="eggNOG" id="COG0840">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_107_16_4"/>
    <dbReference type="OMA" id="ERARMFL"/>
    <dbReference type="OrthoDB" id="429006at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004888">
        <property type="term" value="F:transmembrane signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006935">
        <property type="term" value="P:chemotaxis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00181">
        <property type="entry name" value="Tar_Tsr_LBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035440">
        <property type="entry name" value="4HB_MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004090">
        <property type="entry name" value="Chemotax_Me-accpt_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003660">
        <property type="entry name" value="HAMP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004089">
        <property type="entry name" value="MCPsignal_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003122">
        <property type="entry name" value="Tar_rcpt_lig-bd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00672">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00015">
        <property type="entry name" value="MCPsignal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02203">
        <property type="entry name" value="TarH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00260">
        <property type="entry name" value="CHEMTRNSDUCR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00304">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00283">
        <property type="entry name" value="MA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47170">
        <property type="entry name" value="SSF47170"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50111">
        <property type="entry name" value="CHEMOTAXIS_TRANSDUC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50885">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0145">Chemotaxis</keyword>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0488">Methylation</keyword>
    <keyword evidence="11" id="KW-0614">Plasmid</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0807">Transducer</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="12"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="193"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="domain" description="HAMP" evidence="10">
        <location>
            <begin position="215"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="domain" description="Methyl-accepting transducer" evidence="9">
        <location>
            <begin position="272"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="535"/>
            <end position="605"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="472"/>
            <end position="510"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004429"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022500"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00029447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ABF10550.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="605" mass="63845" checksum="A935AC3E7BA1419D" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LH29</accession>
    <name>Q1LH29_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Phage integrase / Tyr recombinase protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">int</name>
        <name evidence="3" type="ordered locus">Rmet_3675</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10547.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518198.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3675"/>
    <dbReference type="EnsemblBacteria" id="ABF10547">
        <property type="protein sequence ID" value="ABF10547"/>
        <property type="gene ID" value="Rmet_3675"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3675"/>
    <dbReference type="eggNOG" id="COG4974">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_068392_0_0_4"/>
    <dbReference type="OMA" id="WGAFLTW"/>
    <dbReference type="OrthoDB" id="1085477at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.443.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011010">
        <property type="entry name" value="DNA_brk_join_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013762">
        <property type="entry name" value="Integrase-like_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002104">
        <property type="entry name" value="Integrase_catalytic"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00589">
        <property type="entry name" value="Phage_integrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56349">
        <property type="entry name" value="SSF56349"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51898">
        <property type="entry name" value="TYR_RECOMBINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tyr recombinase" evidence="2">
        <location>
            <begin position="147"/>
            <end position="346"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF10547.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="358" mass="39050" checksum="82E7577E7AFC82D7" modified="2006-05-30" version="1">MTQASADFVTADGETPATSGDLFDADLDAWRKHPDRAFDGWLARHGFRHGTAVVYRAMWGKLLRWSGEQGLTPLTWSAAQIGAFLDAQNLHKRHRYRYARLIERVFHHLSQLQQNLHNPASQAVKSHLADGENDPTAFLLPGERDLLVARILAPAPEPVAEAARPDSQAISPKQWKRARDVALLAVLLGGGLKVAEARALRVEAIEGLGGGAMAIRMVRTDNGRAYTVPLFGFVHAPLRRWLETRAAAETLGDLVFPAMTNGRAMHAASVYRRVEILLEEAGVLAGRSERASPQTLRNTCGAMHFDAGTPPAAVAQLLGMRDMESGWRLRAAYEAWQARTGLPVMGRPLSRPNGGSGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LH31</accession>
    <name>Q1LH31_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Transcriptional regulator, PadR-like family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_3673</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10545.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518197.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3673"/>
    <dbReference type="EnsemblBacteria" id="ABF10545">
        <property type="protein sequence ID" value="ABF10545"/>
        <property type="gene ID" value="Rmet_3673"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3673"/>
    <dbReference type="eggNOG" id="COG1695">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_089258_1_4_4"/>
    <dbReference type="OMA" id="QFGHYLV"/>
    <dbReference type="OrthoDB" id="1629615at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018309">
        <property type="entry name" value="Tscrpt_reg_PadR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005149">
        <property type="entry name" value="Tscrpt_reg_PadR_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03551">
        <property type="entry name" value="PadR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10400">
        <property type="entry name" value="Vir_act_alpha_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PadR" evidence="1">
        <location>
            <begin position="8"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="domain" description="Vir_act_alpha_C" evidence="2">
        <location>
            <begin position="100"/>
            <end position="180"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF10400"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF10545.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="189" mass="21423" checksum="671D0728D5C61598" modified="2006-05-30" version="1">MSTQHALLISLIEKPSSGYDLARRFDKSIGYFWHATHQQIYRELGRMAELGWIASDEPEVTVDGDGAEKKGRKKVYRVLPAGHDELVRWVLAPVQGLDQREEILVKLRADAAIGPLGLDKEMGRLIALHEARLETYRGIERKDFSGTLDRRQQIQYALLQRGIQFQESWLAWGRSLLPLLTATGSGVKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="97" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LH72</accession>
    <name>Q1LH72_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Aldehyde dehydrogenase family protein</fullName>
            <ecNumber evidence="6">1.2.1.3</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Rmet_3632</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="7">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="7">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the aldehyde dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.2.1.3" evidence="6"/>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10504.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518161.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3632"/>
    <dbReference type="EnsemblBacteria" id="ABF10504">
        <property type="protein sequence ID" value="ABF10504"/>
        <property type="gene ID" value="Rmet_3632"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3632"/>
    <dbReference type="eggNOG" id="COG1012">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005391_1_0_4"/>
    <dbReference type="OMA" id="NDDLGEV"/>
    <dbReference type="OrthoDB" id="744602at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004029">
        <property type="term" value="F:aldehyde dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043878">
        <property type="term" value="F:glyceraldehyde-3-phosphate dehydrogenase (NAD+) (non-phosphorylating) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.309.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.605.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016161">
        <property type="entry name" value="Ald_DH/histidinol_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016163">
        <property type="entry name" value="Ald_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016160">
        <property type="entry name" value="Ald_DH_CS_CYS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029510">
        <property type="entry name" value="Ald_DH_CS_GLU"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016162">
        <property type="entry name" value="Ald_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015590">
        <property type="entry name" value="Aldehyde_DH_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00171">
        <property type="entry name" value="Aldedh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53720">
        <property type="entry name" value="SSF53720"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00070">
        <property type="entry name" value="ALDEHYDE_DEHYDR_CYS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00687">
        <property type="entry name" value="ALDEHYDE_DEHYDR_GLU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 4 6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-0614">Plasmid</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aldedh" evidence="5">
        <location>
            <begin position="13"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="active site" evidence="2">
        <location>
            <position position="249"/>
        </location>
    </feature>
    <feature type="active site" evidence="3">
        <location>
            <position position="283"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABF10504.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="482" mass="51639" checksum="3207B1C989304E5E" modified="2006-05-30" version="1">MTETYRNYIDGEWCDSASGRTVDNVNPADTRDIVSRHAASDARDAAAAVAAAAAAFDGWKKTPIGKRAKILNDAAAHLEANADTIATELTREEGKALNLARDEVMRSAQTLRFYAVEGQTFSGESYPNDDPDMLVYSLREPLGVVTVISPWNFPVSIPARKIAPALITGNTVVFKPSSDAPLSGYRLAEAFVRAGIPKGVLNFLTGSAAEVGPTIVESREVRAVSFTGSTSAGEQIHKSVPMTTRTQMELGGKNPLIVMEDADLDRAVDLAVKGGLSLSGQACTGTSRILVMRDVKQAFTEKLVAKVKALKIGSGMTAGMDLGPLATRKQLETVLSYIAIGKQEATLLCGGEQLSDGDFAHGYYVAPTVFTDVTQSMRIAREEIFGPVLAIIEVDSYADAIAQANDTEYGLSAAIATRNPRYMHDFTRDIESGTVKINRTTTGNLVNAPFGGLKRSSTSTFRESGRAGLEFYTQIKTVYQGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LHA3</accession>
    <name>Q1LHA3_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_3601</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10473.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518016.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3601"/>
    <dbReference type="EnsemblBacteria" id="ABF10473">
        <property type="protein sequence ID" value="ABF10473"/>
        <property type="gene ID" value="Rmet_3601"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3601"/>
    <dbReference type="eggNOG" id="COG3687">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_051636_0_1_4"/>
    <dbReference type="OMA" id="WHALEEC"/>
    <dbReference type="OrthoDB" id="1601417at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016516">
        <property type="entry name" value="UCP07580"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39456">
        <property type="entry name" value="PTHR39456"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10118">
        <property type="entry name" value="Metal_hydrol"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF007580">
        <property type="entry name" value="UCP07580"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="181"/>
            <end position="204"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF10473.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="290" mass="33387" checksum="F96C14E37A0B4AE2" modified="2006-05-30" version="1">MMPVRRDVHPHLPPERICDWHQRGRHVTHFINALSIFFPAGERFFMDSVRNYRDRIADPELKQAVAGFIGQEAMHTREHILYNRLLDDVGLPAGKLDSRVASLLNLLRKILPKSWQLAHTIALEHYTAMLAAGVLADPRQMGDSEPGYRQVWTWHALEETEHKAVAYDVWNVVMKPGPYRYFVRTFTMLTTTVTFWAMVFVFHVRLVMADKSCRNKLLGFGSVMNFLWGSPGTLRKMIPEWFAYFRPGFHPWDDDNRAELSRIAPLISEIEDTAIRAGASTRAPSIRSVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LHF5</accession>
    <name>Q1LHF5_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Periplasmic tricarboxylate binding receptor (TctC)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">tctC</name>
        <name evidence="4" type="ordered locus">Rmet_3549</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Periplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the UPF0065 (bug) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10421.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008641004.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3549"/>
    <dbReference type="EnsemblBacteria" id="ABF10421">
        <property type="protein sequence ID" value="ABF10421"/>
        <property type="gene ID" value="Rmet_3549"/>
    </dbReference>
    <dbReference type="GeneID" id="60822653"/>
    <dbReference type="KEGG" id="rme:Rmet_3549"/>
    <dbReference type="eggNOG" id="COG3181">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045683_1_0_4"/>
    <dbReference type="OMA" id="MIPANPG"/>
    <dbReference type="OrthoDB" id="988124at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042597">
        <property type="term" value="C:periplasmic space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005064">
        <property type="entry name" value="BUG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042100">
        <property type="entry name" value="Bug_dom1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42928">
        <property type="entry name" value="PTHR42928"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03401">
        <property type="entry name" value="TctC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017082">
        <property type="entry name" value="YflP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0675">Receptor</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193462" evidence="3">
        <location>
            <begin position="30"/>
            <end position="322"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF10421.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="322" mass="33179" checksum="0AFE58F4166E3732" modified="2006-05-30" version="1">MNRSFARFTHAFVASATLAAAVVAAPAYAVDSLKVMIGANPGGGFDQTGRTIGAAMVAAGAAKSASYDNKGGAGGTIGLTQFVNTDKGNPNALIVTGAVMVGAIETNHPPVTLKNATPIARLFADTMVLTVPASSPIKSIKDLTTQLKANPGSVSWGGGSKGSIDHILAGLIAKEAGVDPKKINYVPFQGGGEASASILGGHVTVGIAGVSEFLPFIKTGKMRALAVTSKDRTADIPTLKEQGINVEIYNWRGVYGAPGISAEQRKAMIDAVVKGTENPVWKDALQKNDWTPFLLTGDEFGKFVDAESARLGGTLRELGVAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="56" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LHF6</accession>
    <name>Q1LHF6_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_3548</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10420.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517966.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF10420">
        <property type="protein sequence ID" value="ABF10420"/>
        <property type="gene ID" value="Rmet_3548"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3548"/>
    <dbReference type="HOGENOM" id="CLU_134415_0_0_4"/>
    <dbReference type="OMA" id="WKSTMAA"/>
    <dbReference type="OrthoDB" id="1548857at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="48"/>
            <end position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF10420.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="164" mass="18694" checksum="99C9A9DD7F626C10" modified="2006-05-30" version="1">MDTIIKASDGWVPELGAASDRGLWKSTMAAANQALEAAKGMQAAVSQSLKLQRKITALRDELHRMEAERDLYRDLHSRTVDELNHTLDLSPDEWQRLRADNETLQIRHRAYKLLVQHYARSGLAIEPAVFAEQRSRVQQHILFQRRKGIPVSVITADDIAFLVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="100" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LHG3</accession>
    <name>Q1LHG3_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Aquaporin-like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Rmet_3541</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the MIP/aquaporin (TC 1.A.8) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10413.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3541"/>
    <dbReference type="EnsemblBacteria" id="ABF10413">
        <property type="protein sequence ID" value="ABF10413"/>
        <property type="gene ID" value="Rmet_3541"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3541"/>
    <dbReference type="eggNOG" id="COG0580">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020019_8_0_4"/>
    <dbReference type="OMA" id="FHWLCRK"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015267">
        <property type="term" value="F:channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1080.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023271">
        <property type="entry name" value="Aquaporin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034294">
        <property type="entry name" value="Aquaporin_transptr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000425">
        <property type="entry name" value="MIP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45724">
        <property type="entry name" value="PTHR45724"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00230">
        <property type="entry name" value="MIP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00783">
        <property type="entry name" value="MINTRINSICP"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81338">
        <property type="entry name" value="SSF81338"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="61"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="92"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="139"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="178"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="202"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="249"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="34"/>
            <end position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU000477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ABF10413.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="294" mass="30375" checksum="6AC2D50A8E2F9CBD" modified="2006-05-30" version="1">MRPAIQACHSIPAVRSLHPGAALTGFAPARNTARSQFPHDSARAMPDTTPTMRTPTTARRLVAEGLGTALLIAIVVGSGIRAERLAAGDTALALLCNALATGAGLVALLVSLGPVSGGHFNPVVTLSNLIQGNLSPREALGYVLAQLSGAVFGVMAAHAMFGLPLLAAATQARTGPPMWWSEFLATFGLIGLGLATSRHRPGLVPFVVAGYITAGYWFTSSTSFANPALTIACALTDTFTGIRAIDASMFILAQIFGSANAALLFQWLCPAPMAAASSPPAQRWQGRTELTSAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LI14</accession>
    <name>Q1LI14_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Protein translocase subunit SecE</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7 8" type="primary">secE</name>
        <name evidence="8" type="ordered locus">Rmet_3340</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Essential subunit of the Sec protein translocation channel SecYEG. Clamps together the 2 halves of SecY. May contact the channel plug during translocation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Component of the Sec protein translocase complex. Heterotrimer consisting of SecY, SecE and SecG subunits. The heterotrimers can form oligomers, although 1 heterotrimer is thought to be able to translocate proteins. Interacts with the ribosome. Interacts with SecDF, and other proteins may be involved. Interacts with SecA.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the SecE/SEC61-gamma family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10212.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008649200.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3340"/>
    <dbReference type="EnsemblBacteria" id="ABF10212">
        <property type="protein sequence ID" value="ABF10212"/>
        <property type="gene ID" value="Rmet_3340"/>
    </dbReference>
    <dbReference type="GeneID" id="60826577"/>
    <dbReference type="KEGG" id="rme:Rmet_3340"/>
    <dbReference type="eggNOG" id="COG0690">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_113663_0_2_4"/>
    <dbReference type="OMA" id="FLWLTDK"/>
    <dbReference type="OrthoDB" id="1974284at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008320">
        <property type="term" value="F:protein transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0065002">
        <property type="term" value="P:intracellular protein transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009306">
        <property type="term" value="P:protein secretion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006605">
        <property type="term" value="P:protein targeting"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043952">
        <property type="term" value="P:protein transport by the Sec complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.1030">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00422">
        <property type="entry name" value="SecE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005807">
        <property type="entry name" value="SecE_bac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038379">
        <property type="entry name" value="SecE_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001901">
        <property type="entry name" value="Translocase_SecE/Sec61-g"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33910">
        <property type="entry name" value="PTHR33910"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00584">
        <property type="entry name" value="SecE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01650">
        <property type="entry name" value="SECETRNLCASE"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00964">
        <property type="entry name" value="secE_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="7" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 7" id="KW-0653">Protein transport</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7" id="KW-0811">Translocation</keyword>
    <keyword evidence="2 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="1 7" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="16"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="42"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="89"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00422"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ABF10212.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="126" mass="13531" checksum="41F0889C360E5A63" modified="2006-05-30" version="1">MANPNVETVGASSGKWLLGVAVLLVVAGVIGFYALAQQPSYVRGAALFAGIAAGVVVALLSAPGKDFVGFARESYREVRKVVWPTRKEAGQMTGLVFVFVVIMALFLWSADKLIEWVIFSLVLGWK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2007-10-02" modified="2022-05-25" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LI60</accession>
    <name>RS13_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">30S ribosomal protein S13</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpsM</name>
        <name type="ordered locus">Rmet_3294</name>
    </gene>
    <organism>
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Located at the top of the head of the 30S subunit, it contacts several helices of the 16S rRNA. In the 70S ribosome it contacts the 23S rRNA (bridge B1a) and protein L5 of the 50S subunit (bridge B1b), connecting the 2 subunits; these bridges are implicated in subunit movement. Contacts the tRNAs in the A and P-sites.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the 30S ribosomal subunit. Forms a loose heterodimer with protein S19. Forms two bridges to the 50S subunit in the 70S ribosome.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uS13 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10166.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008642961.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q1LI60"/>
    <dbReference type="SMR" id="Q1LI60"/>
    <dbReference type="STRING" id="266264.Rmet_3294"/>
    <dbReference type="EnsemblBacteria" id="ABF10166">
        <property type="protein sequence ID" value="ABF10166"/>
        <property type="gene ID" value="Rmet_3294"/>
    </dbReference>
    <dbReference type="GeneID" id="60826623"/>
    <dbReference type="KEGG" id="rme:Rmet_3294"/>
    <dbReference type="eggNOG" id="COG0099">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_103849_1_2_4"/>
    <dbReference type="OMA" id="YRGLRHK"/>
    <dbReference type="OrthoDB" id="1772647at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.910.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01315">
        <property type="entry name" value="Ribosomal_S13_S18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027437">
        <property type="entry name" value="30s_Rbsml_prot_S13_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001892">
        <property type="entry name" value="Ribosomal_S13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010979">
        <property type="entry name" value="Ribosomal_S13-like_H2TH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019980">
        <property type="entry name" value="Ribosomal_S13_bac-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018269">
        <property type="entry name" value="Ribosomal_S13_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10871:SF1">
        <property type="entry name" value="PTHR10871:SF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00416">
        <property type="entry name" value="Ribosomal_S13"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002134">
        <property type="entry name" value="Ribosomal_S13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46946">
        <property type="entry name" value="SSF46946"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03631">
        <property type="entry name" value="uS13_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00646">
        <property type="entry name" value="RIBOSOMAL_S13_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50159">
        <property type="entry name" value="RIBOSOMAL_S13_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <keyword id="KW-0820">tRNA-binding</keyword>
    <feature type="chain" id="PRO_0000306686" description="30S ribosomal protein S13">
        <location>
            <begin position="1"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="91"/>
            <end position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="121" mass="13624" checksum="D50510EFC2C76894" modified="2006-05-30" version="1">MARIAGVNIPNHKHTEIGLTAIYGIGRSRARKICEATGVPFDKKVKDLTDADLEKLRDEVGKVTVEGDLRRETTMNIKRLMDLGCYRGMRHRKGLPMRGQRTRTNARTRKGPRKAGVALKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="56" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LID2</accession>
    <name>Q1LID2_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_3222</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10094.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517705.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3222"/>
    <dbReference type="EnsemblBacteria" id="ABF10094">
        <property type="protein sequence ID" value="ABF10094"/>
        <property type="gene ID" value="Rmet_3222"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3222"/>
    <dbReference type="HOGENOM" id="CLU_2734522_0_0_4"/>
    <dbReference type="OMA" id="SACSHIQ"/>
    <dbReference type="OrthoDB" id="1931937at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF10094.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="73" mass="8404" checksum="0827A88DFAF54718" modified="2006-05-30" version="1">MSKAALTLRFKCKKCTKPVTLYLQKTSACSHILPYQGFCKCGEMMRHAIGDKDAVESFVNSMDNSWMHHHHHH</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="68" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LIP0</accession>
    <name>Q1LIP0_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Oligopeptide transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Rmet_3114</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09986.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517609.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3114"/>
    <dbReference type="EnsemblBacteria" id="ABF09986">
        <property type="protein sequence ID" value="ABF09986"/>
        <property type="gene ID" value="Rmet_3114"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3114"/>
    <dbReference type="eggNOG" id="COG1297">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018238_1_1_4"/>
    <dbReference type="OMA" id="TPTAYVW"/>
    <dbReference type="OrthoDB" id="809815at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035673">
        <property type="term" value="F:oligopeptide transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004814">
        <property type="entry name" value="Oligopep_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004813">
        <property type="entry name" value="OPT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045035">
        <property type="entry name" value="YSL-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31645">
        <property type="entry name" value="PTHR31645"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03169">
        <property type="entry name" value="OPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00728">
        <property type="entry name" value="OPT_sfam"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00733">
        <property type="entry name" value="TIGR00733"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="21"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="46"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="81"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="106"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="169"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="195"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="227"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="268"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="323"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="355"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="390"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="423"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="464"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="505"/>
            <end position="522"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="527"/>
            <end position="543"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="563"/>
            <end position="586"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="613"/>
            <end position="635"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="655"/>
            <end position="673"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABF09986.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="676" mass="69314" checksum="7A0BB8944DA3CB81" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LJ09</accession>
    <name>Q1LJ09_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Phosphite transport system-binding protein ptxB</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">ptxB</name>
        <name evidence="5" type="ordered locus">Rmet_2994</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the phosphate/phosphite/phosphonate binding protein family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09867.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_003056094.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2994"/>
    <dbReference type="EnsemblBacteria" id="ABF09867">
        <property type="protein sequence ID" value="ABF09867"/>
        <property type="gene ID" value="Rmet_2994"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2994"/>
    <dbReference type="eggNOG" id="COG3221">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_051472_6_2_4"/>
    <dbReference type="OMA" id="YIYPSLW"/>
    <dbReference type="OrthoDB" id="988224at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043190">
        <property type="term" value="C:ATP-binding cassette (ABC) transporter complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005770">
        <property type="entry name" value="PhnD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001638">
        <property type="entry name" value="Solute-binding_3/MltF_N"/>
    </dbReference>
    <dbReference type="SMART" id="SM00062">
        <property type="entry name" value="PBPb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01098">
        <property type="entry name" value="3A0109s03R"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193502" evidence="3">
        <location>
            <begin position="27"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="domain" description="PBPb" evidence="4">
        <location>
            <begin position="33"/>
            <end position="270"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00062"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABF09867.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="292" mass="31673" checksum="0115C4F2B086C1A1" modified="2006-05-30" version="1">MLNRRNFSLVVSAASFAALLPLWAHAQGKDPSKLRVALLPDENAASIIQNAQPLKRYLEQQLKKDVEIVVTTDYSSMIEAMRFGRIEVAYFGPFSYVLAKSKASGIEPFAVGVERGSPTYQSVLIATAGGPVKTLEDVRGKPFGFGDQASTSSHLAPRAHLLKKYKLDGEKDYRPVHLGAHDAVARAVQSGQIPAGALSKPILDNLIARGSIDATKIVQLDLSAPIPNYPVVMQGDLKPELKAAIRSAFLDMKDAEVLKAFRVQAFAATDDAAYDVLRDTATILKLDLGRMQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-08-12" version="57" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LJH8</accession>
    <name>Q1LJH8_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Plasmid encoded RepA protein CMGI-5</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">repA</name>
        <name evidence="2" type="ordered locus">Rmet_2825</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09698.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2825"/>
    <dbReference type="EnsemblBacteria" id="ABF09698">
        <property type="protein sequence ID" value="ABF09698"/>
        <property type="gene ID" value="Rmet_2825"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2825"/>
    <dbReference type="eggNOG" id="ENOG502ZB3T">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_051492_1_0_4"/>
    <dbReference type="OMA" id="VEGREFM"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006881">
        <property type="entry name" value="RepA_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04796">
        <property type="entry name" value="RepA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF09698.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="329" mass="36172" checksum="8939673252DBBC66" modified="2006-05-30" version="1">MRSNIDAGPATIGNPIDSAPRLGSACQKGNRDAQFTSEALALEAESAKNAGAMGFMARVLVQTTLPHSIQNGPRYTRRNGTLTVSITDLSGAGLPYGSYPRLLLIWLTTEACRTKNRQLELGPSLSSFMAELGLMSTGGRWGTIPRLKDQMARLFSAAISATSTSGESTALHHHSGKNLLVADDFDLWWSPHSPDQTTLWRSTVTLSEALFQQVIDRPVPVDLRAVKLLKKSPMALDLYAWSTYRVSYLRRSTLIPWESMMLSLGTGYPETAQGKADFKRKFVQALRKVSTVYPALRASPNERGLLLQPSTTHIPRQSRFDKGGFSCRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LJS8</accession>
    <name>Q1LJS8_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Transferase (Hexapeptide repeat)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_2725</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09598.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2725"/>
    <dbReference type="EnsemblBacteria" id="ABF09598">
        <property type="protein sequence ID" value="ABF09598"/>
        <property type="gene ID" value="Rmet_2725"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2725"/>
    <dbReference type="eggNOG" id="COG0110">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_081811_2_0_4"/>
    <dbReference type="OMA" id="TIGHDCI"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03360">
        <property type="entry name" value="LbH_AT_putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001451">
        <property type="entry name" value="Hexapep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018357">
        <property type="entry name" value="Hexapep_transf_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020019">
        <property type="entry name" value="Sia_OAcTrfase_NeuD-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011004">
        <property type="entry name" value="Trimer_LpxA-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00132">
        <property type="entry name" value="Hexapep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51161">
        <property type="entry name" value="SSF51161"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03570">
        <property type="entry name" value="NeuD_NnaD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00101">
        <property type="entry name" value="HEXAPEP_TRANSFERASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF09598.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="181" mass="18874" checksum="5D4F945D91B8E3E4" modified="2006-05-30" version="1">MGKRHFFDDAWPNINMNGRWQVVGDFSALLAYAQVFDAAFVSIGNCRVRWAKHQALLAAGIPIATVIHPRAYISQFARLGIGSIAMAGVVINADVGDGGIVNTDATIDHDCILDNGVHVSPGAHLSGSVSVGKCSWIAVGASIKQGITVGSDAIVGAGAVVVRPVRDGVTVMGCPAREQRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="56" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LKA0</accession>
    <name>Q1LKA0_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_2549</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09426.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2549"/>
    <dbReference type="EnsemblBacteria" id="ABF09426">
        <property type="protein sequence ID" value="ABF09426"/>
        <property type="gene ID" value="Rmet_2549"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2549"/>
    <dbReference type="eggNOG" id="ENOG5033JTP">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_860165_0_0_4"/>
    <dbReference type="OMA" id="QGTWMAT"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="28"/>
            <end position="47"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF09426.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="323" mass="33430" checksum="BC87C3CF5F6AEFCC" modified="2006-05-30" version="1">MDGAAADHQLPCSRIHRISCSRTAESNSVTTIPLTPPPAHQNSSTKVVGGTTSITFRAGGGATTTTTHGTVSASDLTPYGDDDWRSTAKNSFGNPASKITEDSVVVIDGMSAQVGTLVKAGVLVETTDGFELASKGTPQDDTEEETQVEPGAMPADVVDAVNSALGGMSDSTVQKAGSIGIAAAVGDMTIDDVVAGVALDTGMEPAEAAQRTQFVVDAYQGQADNFVVNHLGIPSGDLQNFYAFCRQPENKGALQTAVQQQVFGNSMAAFKPLVEAYMSNVAPSARALASRGFETKTAPNGEEMVRIQGTWMATKVAAKVGLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="95" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LKB9</accession>
    <name>Q1LKB9_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Isovaleryl-CoA dehydrogenase (IVDHase)</fullName>
            <ecNumber evidence="8">1.3.8.4</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">ivd</name>
        <name evidence="8" type="ordered locus">Rmet_2530</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the acyl-CoA dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.3.8.4" evidence="8"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09407.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517106.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2530"/>
    <dbReference type="EnsemblBacteria" id="ABF09407">
        <property type="protein sequence ID" value="ABF09407"/>
        <property type="gene ID" value="Rmet_2530"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2530"/>
    <dbReference type="eggNOG" id="COG1960">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018204_0_3_4"/>
    <dbReference type="OMA" id="GYRGMKE"/>
    <dbReference type="OrthoDB" id="760677at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008470">
        <property type="term" value="F:isovaleryl-CoA dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.540.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.110.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006089">
        <property type="entry name" value="Acyl-CoA_DH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006091">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046373">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036250">
        <property type="entry name" value="AcylCo_DH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009075">
        <property type="entry name" value="AcylCo_DH/oxidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013786">
        <property type="entry name" value="AcylCoA_DH/ox_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037069">
        <property type="entry name" value="AcylCoA_DH/ox_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009100">
        <property type="entry name" value="AcylCoA_DH/oxidase_NM_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00441">
        <property type="entry name" value="Acyl-CoA_dh_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02770">
        <property type="entry name" value="Acyl-CoA_dh_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02771">
        <property type="entry name" value="Acyl-CoA_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47203">
        <property type="entry name" value="SSF47203"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56645">
        <property type="entry name" value="SSF56645"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00072">
        <property type="entry name" value="ACYL_COA_DH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00073">
        <property type="entry name" value="ACYL_COA_DH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0274">FAD</keyword>
    <keyword evidence="3" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Acyl-CoA_dh_N" evidence="7">
        <location>
            <begin position="10"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA_dh_M" evidence="6">
        <location>
            <begin position="123"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA_dh_1" evidence="5">
        <location>
            <begin position="230"/>
            <end position="378"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF02770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF02771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ABF09407.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="378" mass="42199" checksum="0E9CD1A8069DBAC0" modified="2006-05-30" version="1">MEVVRSVYREDHEQFRTTVRRFLERECVPRQEQWLADGIVDRETWLKAGREGLLCTALPTEYGGGGGDFGHAAIIAEEMAYAGVSVSFGLHSDIVAPYIARLGTEEQKQRWLPDICAGKTILAVAITEPGGGSDVKACRTTAVRDGDEWVINGSKTFISNGFCSDMVMVVCKTDPAAGARGVSLILVETNREGFRRGRKLHKVGMHSQDTAELFFDNVRVPAGNLLGEVNKGFGYLMHDLAQERFIIAVTAAAKIERLLEQTIEYVKDRKAFGGTVWDFQNTKFKLADVKANAVALRTMVDYYLGEHMRRRLSGEEAAIAKLHATEVQWKCIDDMVQLHGGYGYMLEYPIARAFVDMRVARVYGGSNEVMRDIITRKM</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="58" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LKY6</accession>
    <name>Q1LKY6_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_2311</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09190.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_003098941.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2311"/>
    <dbReference type="EnsemblBacteria" id="ABF09190">
        <property type="protein sequence ID" value="ABF09190"/>
        <property type="gene ID" value="Rmet_2311"/>
    </dbReference>
    <dbReference type="GeneID" id="56409818"/>
    <dbReference type="GeneID" id="61526437"/>
    <dbReference type="KEGG" id="rme:Rmet_2311"/>
    <dbReference type="eggNOG" id="COG4860">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_070054_0_0_4"/>
    <dbReference type="OMA" id="FFDTLGQ"/>
    <dbReference type="OrthoDB" id="1732700at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF09190.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="274" mass="30565" checksum="07399D0CD2AB7403" modified="2006-05-30" version="1">MDRSLIKSMMPSLVAGHVPRNVRSFKYRVFDDQPLSSTLGFAIDPQPFDGKVVAATDDAIVVKLKPSEFAVLDPSLVTTVPAEGAKVHVQPYARRRFDGLRADTPEVITEETSDGTPYTITRHILGSAPAKLPIPTPQCMELGQLIEQLEEMPAPDRFRRITHMLVDAGARDFTWVDPTPSKIIETPPAISFTVSTAKFEGRVTILYDRGGDTYVVELHRQNGESVELVDRHDEVYFDMLGEVLERLIDDGRWRQIDVSILDAKAARKRQAVPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="53" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LL39</accession>
    <name>Q1LL39_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_2258</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09137.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516960.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2258"/>
    <dbReference type="EnsemblBacteria" id="ABF09137">
        <property type="protein sequence ID" value="ABF09137"/>
        <property type="gene ID" value="Rmet_2258"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2258"/>
    <dbReference type="HOGENOM" id="CLU_2668424_0_0_4"/>
    <dbReference type="OMA" id="MKPANAR"/>
    <dbReference type="OrthoDB" id="1972201at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF09137.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="75" mass="7822" checksum="F2B2DF1BBC6D4455" modified="2006-05-30" version="1">MSKPIGSRKAATAYSDIDMLAQHVDGFDAQQYFDQQVEVETVAAAQRWPLLARVMGCAALAEAEPAVAVGPSGGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="62" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LL43</accession>
    <name>Q1LL43_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_2254</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09133.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516956.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2254"/>
    <dbReference type="EnsemblBacteria" id="ABF09133">
        <property type="protein sequence ID" value="ABF09133"/>
        <property type="gene ID" value="Rmet_2254"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2254"/>
    <dbReference type="eggNOG" id="ENOG5030X9V">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2828118_0_0_4"/>
    <dbReference type="OMA" id="WLPRWTA"/>
    <dbReference type="OrthoDB" id="2178437at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF09133.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="66" mass="7725" checksum="BC97B21D73D3C6FE" modified="2006-05-30" version="1">MPIFLGYFVWGLILAAPLAWLTWRFDLLRWLPRWTAPRPRRSVRLPPQLDALVIRGKRRIGGGDQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LL78</accession>
    <name>Q1LL78_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">ABC-type nitrate/sulfonate/bicarbonate transport systems periplasmic component</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_2219</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09098.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516926.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2219"/>
    <dbReference type="EnsemblBacteria" id="ABF09098">
        <property type="protein sequence ID" value="ABF09098"/>
        <property type="gene ID" value="Rmet_2219"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2219"/>
    <dbReference type="eggNOG" id="COG0715">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_054373_1_0_4"/>
    <dbReference type="OMA" id="DPRTWIW"/>
    <dbReference type="OrthoDB" id="1635706at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015168">
        <property type="entry name" value="SsuA/THI5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006311">
        <property type="entry name" value="TAT_signal"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09084">
        <property type="entry name" value="NMT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51318">
        <property type="entry name" value="TAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NMT1" evidence="1">
        <location>
            <begin position="58"/>
            <end position="271"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF09084"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF09098.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="341" mass="37047" checksum="154C52B4CC088DA2" modified="2006-05-30" version="1">MSQQPSQKSSRPDNRRRSLLRLAGTTAIAAPALILGRQAWSAPRKLTFAWNQNSFCLTPIVVAQEKGFFEKNGLQVDLINYSGSTDQLLESIATGKADAAVGMIHRWLKPLEAGFDVKIIGSSHGGCVRLVGAKAAGVTNLQALKGKTVGVSDLAAPGKHFFTILLAKNGIDPDRDITWRQYPADLLGVAVDKGEIQAIADGDPNLYLLEKRTNGAYVELATNLTGEYARKVCCVVGARGELVRNDRPTAAALARSIVQATDYVNENPNEAAKVFAKYSPKISPDDLRKLYATLTYTHHPTGVDLQEEIAFYADDFRRIGVLKKSTDAKRLAQHVYANVLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LLD0</accession>
    <name>Q1LLD0_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">TatD Mg-dependent-related deoxyribonuclease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_2167</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09046.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2167"/>
    <dbReference type="EnsemblBacteria" id="ABF09046">
        <property type="protein sequence ID" value="ABF09046"/>
        <property type="gene ID" value="Rmet_2167"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2167"/>
    <dbReference type="eggNOG" id="COG0084">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031506_5_1_4"/>
    <dbReference type="OMA" id="HPQLAHE"/>
    <dbReference type="OrthoDB" id="915852at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016788">
        <property type="term" value="F:hydrolase activity, acting on ester bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001130">
        <property type="entry name" value="TatD-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01026">
        <property type="entry name" value="TatD_DNase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005902">
        <property type="entry name" value="DNase_TatD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="SSF51556"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF09046.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="249" mass="27654" checksum="C335F7B96CC2380A" modified="2006-05-30" version="1">MMDFHCHLDLYPSARTVHTEAARRCEFVWLVTTSPRAFIATSRILQPAPTIIISPGLHPEVAHQRHNELPLLLEEIGRASAVGEVGLDGSARYREHFSVQKAIFEATVERCSELGGRVLSIHSRSAAKEVLDTLERHSAHGTAVLHWFSDSPTQLRRAIELGCWFSIGPAMLRSANGLRLAASLPSERVVPESDGPFAKLDSRPIMPWQAMDIVGPLANVWGLTEDKVRAQLTENGRELRHMMTAENSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LLX8</accession>
    <name>Q1LLX8_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Thioredoxin peroxidase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">bcp</name>
        <name evidence="6" type="ordered locus">Rmet_1969</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08848.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516693.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1969"/>
    <dbReference type="EnsemblBacteria" id="ABF08848">
        <property type="protein sequence ID" value="ABF08848"/>
        <property type="gene ID" value="Rmet_1969"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1969"/>
    <dbReference type="eggNOG" id="COG1225">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_042529_14_1_4"/>
    <dbReference type="OMA" id="RVVVYFY"/>
    <dbReference type="OrthoDB" id="1996723at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004601">
        <property type="term" value="F:peroxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000866">
        <property type="entry name" value="AhpC/TSA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024706">
        <property type="entry name" value="Peroxiredoxin_AhpC-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00578">
        <property type="entry name" value="AhpC-TSA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000239">
        <property type="entry name" value="AHPC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="SSF52833"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="1 6" id="KW-0575">Peroxidase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thioredoxin" evidence="5">
        <location>
            <begin position="3"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="active site" description="Cysteine sulfenic acid (-SOH) intermediate" evidence="4">
        <location>
            <position position="44"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00032824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR000239-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABF08848.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="153" mass="17260" checksum="5F6C3A5EA4CC5FCE" modified="2006-05-30" version="1">MTVSLNKAIPDFQAPATGGDFRLADQRGKTTVLYFYPKDNTPGCTTEAMNFRDQYDDFEKAGAAVYGISRDSMKSHENFKAKLELPFELISDADEAICQLFDVIKMKKMYGKDVRGIERSTFILDAKGVLRHEMRGIKVPNHVDEVLEIVKGM</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LM48</accession>
    <name>Q1LM48_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Pyruvate dehydrogenase (Pyruvate oxidase), thiamin-dependent, FAD-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">poxB</name>
        <name evidence="6" type="ordered locus">Rmet_1899</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the TPP enzyme family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08778.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516630.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1899"/>
    <dbReference type="EnsemblBacteria" id="ABF08778">
        <property type="protein sequence ID" value="ABF08778"/>
        <property type="gene ID" value="Rmet_1899"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1899"/>
    <dbReference type="eggNOG" id="COG0028">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013748_3_0_4"/>
    <dbReference type="OMA" id="DRNAFQD"/>
    <dbReference type="OrthoDB" id="351701at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030976">
        <property type="term" value="F:thiamine pyrophosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044281">
        <property type="term" value="P:small molecule metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029035">
        <property type="entry name" value="DHS-like_NAD/FAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012000">
        <property type="entry name" value="Thiamin_PyroP_enz_cen_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012001">
        <property type="entry name" value="Thiamin_PyroP_enz_TPP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000399">
        <property type="entry name" value="TPP-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011766">
        <property type="entry name" value="TPP_enzyme-bd_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02775">
        <property type="entry name" value="TPP_enzyme_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00205">
        <property type="entry name" value="TPP_enzyme_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02776">
        <property type="entry name" value="TPP_enzyme_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52467">
        <property type="entry name" value="SSF52467"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="SSF52518"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00187">
        <property type="entry name" value="TPP_ENZYMES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0786">Thiamine pyrophosphate</keyword>
    <feature type="domain" description="TPP_enzyme_N" evidence="5">
        <location>
            <begin position="4"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="domain" description="TPP_enzyme_M" evidence="3">
        <location>
            <begin position="191"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="domain" description="TPP_enzyme_C" evidence="4">
        <location>
            <begin position="380"/>
            <end position="524"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007812"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU362132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF02775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF02776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABF08778.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="570" mass="61794" checksum="D29E7FECA91D6079" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LMC5</accession>
    <name>Q1LMC5_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Metal-dependent phosphohydrolase, HD subdomain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_1822</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08701.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516551.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1822"/>
    <dbReference type="EnsemblBacteria" id="ABF08701">
        <property type="protein sequence ID" value="ABF08701"/>
        <property type="gene ID" value="Rmet_1822"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1822"/>
    <dbReference type="eggNOG" id="COG2206">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_92_1_4"/>
    <dbReference type="OMA" id="HMAIEYL"/>
    <dbReference type="OrthoDB" id="1094814at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008081">
        <property type="term" value="F:phosphoric diester hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="CDD" id="cd00077">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021812">
        <property type="entry name" value="DUF3391"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003607">
        <property type="entry name" value="HD/PDEase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037522">
        <property type="entry name" value="HD_GYP_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11871">
        <property type="entry name" value="DUF3391"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00471">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51832">
        <property type="entry name" value="HD_GYP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HD-GYP" evidence="1">
        <location>
            <begin position="128"/>
            <end position="324"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF08701.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="395" mass="44207" checksum="3E7E076FE9F89AC0" modified="2006-05-30" version="1">MLRRIKAEQLQVGMFVSRLGGPWINHPFWRSRFLVSSDEQIEQILAANVQDVWIDILKGRNLPMQSTPEVADSEVDAQPESGFEDELEHARELIKSGRAVIGSIFNDVRMGHALETNGALLLVDSASTSLSRHSQALLAMARLKAKDDYTYLHSYSVCALMIAVGRTLKLPDGEVRELGLAGLVHDIGKLTLPESLLMKAGDLSPAEYEQMRRHPSAGYRILNEARLYTNIPLDVCVHHHERVDGKGYPFGLFESEISVHAKIAAICDTYDSLTSPRPNHLAWSPARAMEYIAVRVDTLFDRRVFKAFTRTVGIYPLGTVLRLRSGRLAVVCGQNANEPLRPRVMTFYSVSQSAPLPHEVLDLTQSEDSVIAFEDARQWGFTDEQLMAMYAPHVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LMQ2</accession>
    <name>Q1LMQ2_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Chemotaxis sensory transducer</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_1693</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08574.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516424.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1693"/>
    <dbReference type="EnsemblBacteria" id="ABF08574">
        <property type="protein sequence ID" value="ABF08574"/>
        <property type="gene ID" value="Rmet_1693"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1693"/>
    <dbReference type="eggNOG" id="COG0840">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_675924_0_0_4"/>
    <dbReference type="OrthoDB" id="429006at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003660">
        <property type="entry name" value="HAMP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024478">
        <property type="entry name" value="HlyB_4HB_MCP"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12729">
        <property type="entry name" value="4HB_MCP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50885">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="189"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="domain" description="HAMP" evidence="2">
        <location>
            <begin position="213"/>
            <end position="265"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF08574.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="407" mass="43501" checksum="007A2A2D65253B31" modified="2006-05-30" version="1">MKNILIGIKSRLIAGFALFALLLTGVGAFGYYGLSTLGERIEALYASNTTPLVRVASVRAHSLRIRMNLWRAQVEASPQATAQAEKDIAASRAAIEDAWARYYPDGITSPSERELATQINAALKQLLPENDKVLALLRAEDYVAAKEYQDANVAVQADRLNELIDKAINDNAAQAEAAVKESSGMTKTILVMAALLMAAGILLSLVIATLLTRGVTKPPDKALNNAADVSKGKLGQPIVVDTQDEIGRAIDALKLMDERLSATVEEGSRQAGDVGASMQEVISVINRMSDIIGEIAATSDKRSRGGEQIHVAINQIDAAAQHNAALVEEAAAASQSLQEQARRMKEDMMFFRLANGTGAVTVPRASVPKAVAPIVKKVAKTKVMNPSSQFALATLEGKSGHDDWQTF</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="102" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LMU5</accession>
    <name>Q1LMU5_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">RNA polymerase sigma P factor (ECF:RpoE-like)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">rpoP</name>
        <name evidence="4" type="ordered locus">Rmet_1648</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the sigma-70 factor family. ECF subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08531.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1648"/>
    <dbReference type="EnsemblBacteria" id="ABF08531">
        <property type="protein sequence ID" value="ABF08531"/>
        <property type="gene ID" value="Rmet_1648"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1648"/>
    <dbReference type="eggNOG" id="COG1595">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047691_9_3_4"/>
    <dbReference type="OMA" id="AAPMTWM"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016987">
        <property type="term" value="F:sigma factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006352">
        <property type="term" value="P:DNA-templated transcription, initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050896">
        <property type="term" value="P:response to stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039425">
        <property type="entry name" value="RNA_pol_sigma-70-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014284">
        <property type="entry name" value="RNA_pol_sigma-70_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007627">
        <property type="entry name" value="RNA_pol_sigma70_r2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013249">
        <property type="entry name" value="RNA_pol_sigma70_r4_t2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013325">
        <property type="entry name" value="RNA_pol_sigma_r2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013324">
        <property type="entry name" value="RNA_pol_sigma_r3/r4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43133">
        <property type="entry name" value="PTHR43133"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04542">
        <property type="entry name" value="Sigma70_r2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08281">
        <property type="entry name" value="Sigma70_r4_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88659">
        <property type="entry name" value="SSF88659"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88946">
        <property type="entry name" value="SSF88946"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02937">
        <property type="entry name" value="sigma70-ECF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Sigma70_r2" evidence="2">
        <location>
            <begin position="36"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="domain" description="Sigma70_r4_2" evidence="3">
        <location>
            <begin position="139"/>
            <end position="191"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF04542"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF08281"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF08531.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="199" mass="22168" checksum="A5F2851D380D23C3" modified="2006-05-30" version="1">MALSPEPPSSLPDAPTGLDVLLGRVALGDRLALRALYDQVVPKLFGLALRITRRRDWAEDVVQESFVSIWHHAADYRPQVAAPMTWMTTIVRNRALDCLRRAHVARLASATSLEDTPEADLEDEHDGPVELAQASEQARALNRCLQRLDGQQREAVVLAYLQDLSHAELAARLRVPLGTIKSWIRRGLEKLRACLEATP</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LN19</accession>
    <name>Q1LN19_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">L,D-carboxypeptidase A</fullName>
            <ecNumber evidence="6">3.4.17.13</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">ldcA</name>
        <name evidence="6" type="ordered locus">Rmet_1574</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase S66 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.17.13" evidence="6"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08457.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516309.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1574"/>
    <dbReference type="MEROPS" id="S66.002"/>
    <dbReference type="EnsemblBacteria" id="ABF08457">
        <property type="protein sequence ID" value="ABF08457"/>
        <property type="gene ID" value="Rmet_1574"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1574"/>
    <dbReference type="eggNOG" id="COG1619">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_034346_0_1_4"/>
    <dbReference type="OMA" id="MLTQWRL"/>
    <dbReference type="OrthoDB" id="952832at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106415">
        <property type="term" value="F:muramoyltetrapeptide carboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10740">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.30.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027461">
        <property type="entry name" value="Carboxypeptidase_A_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027478">
        <property type="entry name" value="LdcA_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040449">
        <property type="entry name" value="Peptidase_S66_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040921">
        <property type="entry name" value="Peptidase_S66C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003507">
        <property type="entry name" value="S66_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30237">
        <property type="entry name" value="PTHR30237"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02016">
        <property type="entry name" value="Peptidase_S66"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17676">
        <property type="entry name" value="Peptidase_S66C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF028757">
        <property type="entry name" value="LD-carboxypeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF141986">
        <property type="entry name" value="SSF141986"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="SSF52317"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0121">Carboxypeptidase</keyword>
    <keyword evidence="2 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0645">Protease</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase_S66" evidence="4">
        <location>
            <begin position="7"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase_S66C" evidence="5">
        <location>
            <begin position="173"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="3">
        <location>
            <position position="108"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="3">
        <location>
            <position position="204"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="3">
        <location>
            <position position="274"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010233"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR028757-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF02016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF17676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABF08457.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="306" mass="33287" checksum="04046C5BA63A37C0" modified="2006-05-30" version="1">MSQPTEVRLIASSGYPHDVAIAARGCAWLKHHGYHVNNPDVLARRYLRFGGTDAERLADLHAIGTGPARELTLAVRGGYGLGRLLDRIEFGRIAEQASASGTPIVGHSDFTAFQLAYLAKAGGVSFAGPMLLADFGEEHVDSFMWRHFEGILRAPVHDIEVEAAQVAAPATVEGTLWGGNLAMLCTLLGTPYMPNIDGGILFLEDINEPPYRVERMLLQLLHAGVLARQQAIVLGDFSNYRTTDYDNGYNMDAVFDYVREQLRIPVLTGLPFGHCPRKLTLPVGGNARLTARADGFVLSLSGYPTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LNC8</accession>
    <name>Q1LNC8_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Tyrosine-based site-specific recombinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">int</name>
        <name evidence="9" type="ordered locus">Rmet_1465</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08348.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516213.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1465"/>
    <dbReference type="DNASU" id="4038268"/>
    <dbReference type="EnsemblBacteria" id="ABF08348">
        <property type="protein sequence ID" value="ABF08348"/>
        <property type="gene ID" value="Rmet_1465"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1465"/>
    <dbReference type="eggNOG" id="COG0582">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027562_0_4_4"/>
    <dbReference type="OMA" id="GVRAIYN"/>
    <dbReference type="OrthoDB" id="785253at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0075713">
        <property type="term" value="P:establishment of integrated proviral latency"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046718">
        <property type="term" value="P:viral entry into host cell"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044826">
        <property type="term" value="P:viral genome integration into host DNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.443.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.390">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044068">
        <property type="entry name" value="CB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011010">
        <property type="entry name" value="DNA_brk_join_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013762">
        <property type="entry name" value="Integrase-like_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002104">
        <property type="entry name" value="Integrase_catalytic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038488">
        <property type="entry name" value="Integrase_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025166">
        <property type="entry name" value="Integrase_DNA_bind_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010998">
        <property type="entry name" value="Integrase_recombinase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004107">
        <property type="entry name" value="Integrase_SAM-like_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13356">
        <property type="entry name" value="Arm-DNA-bind_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14659">
        <property type="entry name" value="Phage_int_SAM_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00589">
        <property type="entry name" value="Phage_integrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56349">
        <property type="entry name" value="SSF56349"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51900">
        <property type="entry name" value="CB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51898">
        <property type="entry name" value="TYR_RECOMBINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0229">DNA integration</keyword>
    <keyword evidence="3" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="2 6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-1179">Viral genome integration</keyword>
    <keyword evidence="5" id="KW-1160">Virus entry into host cell</keyword>
    <feature type="domain" description="Core-binding (CB)" evidence="8">
        <location>
            <begin position="97"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="domain" description="Tyr recombinase" evidence="7">
        <location>
            <begin position="199"/>
            <end position="384"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ABF08348.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="418" mass="47273" checksum="51823F067673E549" modified="2006-05-30" version="1">MLTDTALRNLKPKSLTYKVSDRDGMYVTVSPANTVTFRYDYRLNGRRETLTIGRYGAGGISLAMARELLLEARKAVLQGISPALEKQRDKRRVRAIKTFGAAMETYLAHTRWADSTRAVRKHIIDRDILPVFQNRLLTEIQAEDLRILCNKVKERGAPATAVQIRDIVKQVYVYAIAHGEKVVNPADSVGPSSIATFVPKDRALSPLEIRLMVQQLETVATYPTIKLALRLILLTLVRKSELIQATWDEVDFERKTWTIPKQRMKGRNPHVVYLSRQALDIFVTLHACAAGSKFVFPSRYDAERCMSNATLNRITMLVAEGAKAKDLPLQPFTVHDLRRTGSTLLNEIGFNRDWIEKCLAHEEGRSSRSVYNKAEYAEQRRHMLQEWANLVDAWGGGQTYVPKLMPENVVVPALSAIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="65" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LP22</accession>
    <name>Q1LP22_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative phosphoanhydride phosphorylase</fullName>
            <ecNumber evidence="3">3.1.3.2</ecNumber>
            <ecNumber evidence="3">3.1.3.26</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_1218</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the MbcT/ParT/Res family.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.2" evidence="3"/>
    <dbReference type="EC" id="3.1.3.26" evidence="3"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08104.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515993.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1218"/>
    <dbReference type="EnsemblBacteria" id="ABF08104">
        <property type="protein sequence ID" value="ABF08104"/>
        <property type="gene ID" value="Rmet_1218"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1218"/>
    <dbReference type="eggNOG" id="ENOG502ZAMM">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_097447_0_0_4"/>
    <dbReference type="OMA" id="DPGVFYG"/>
    <dbReference type="OrthoDB" id="1154993at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008707">
        <property type="term" value="F:4-phytase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003993">
        <property type="term" value="F:acid phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014914">
        <property type="entry name" value="RES_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08808">
        <property type="entry name" value="RES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00953">
        <property type="entry name" value="RES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RES" evidence="2">
        <location>
            <begin position="38"/>
            <end position="172"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF08104.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="220" mass="24815" checksum="DE1A51C652581EA2" modified="2006-05-30" version="1">MPLVDTLEEQAVLEAVLDAGKPVVPLDARHLHYLIYTPFRYPPAAEGSRFRGPQDIGVFYGAEAIRTACAELGFWRWRFLQDSPALPRIDARAQTLFQVSVKTDGVALDTPPFVDDGARWTDPNQHDECQAFGRTAREAAIGLIRYTSARDPVHGACAAILTPRAFDAPEPLATTTWMLTVRRDRVMWQRDDLQQRDSFEFEGAWWDALRQRTSESPSTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-10-07" version="59" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LP53</accession>
    <name>Q1LP53_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_1187</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08073.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF08073">
        <property type="protein sequence ID" value="ABF08073"/>
        <property type="gene ID" value="Rmet_1187"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1187"/>
    <dbReference type="eggNOG" id="ENOG5033ZXX">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2370758_0_0_4"/>
    <dbReference type="OMA" id="TCVATRE"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009962">
        <property type="entry name" value="DUF1488"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036692">
        <property type="entry name" value="Shew3726-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07369">
        <property type="entry name" value="DUF1488"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160272">
        <property type="entry name" value="SSF160272"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF08073.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="88" mass="9922" checksum="C890110B2463A896" modified="2006-05-30" version="1">MMEIKFQEQDRYDINNEGLLFHAIVDGEQVTCVVTREALWEGFSADQVLSLEEAFRAGRETIERAALVLIEQGVPQPIVVKRAHVAPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="107" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LP67</accession>
    <name>Q1LP67_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Two component transcriptional regulator, winged helix family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Rmet_1173</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08059.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1173"/>
    <dbReference type="EnsemblBacteria" id="ABF08059">
        <property type="protein sequence ID" value="ABF08059"/>
        <property type="gene ID" value="Rmet_1173"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1173"/>
    <dbReference type="eggNOG" id="COG0745">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_30_4_4"/>
    <dbReference type="OMA" id="EFNILHF"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00383">
        <property type="entry name" value="trans_reg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001867">
        <property type="entry name" value="OmpR/PhoB-type_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016032">
        <property type="entry name" value="Sig_transdc_resp-reg_C-effctor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039420">
        <property type="entry name" value="WalR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48111">
        <property type="entry name" value="PTHR48111"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00486">
        <property type="entry name" value="Trans_reg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00862">
        <property type="entry name" value="Trans_reg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46894">
        <property type="entry name" value="SSF46894"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51755">
        <property type="entry name" value="OMPR_PHOB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Response regulatory" evidence="4">
        <location>
            <begin position="25"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="domain" description="OmpR/PhoB-type" evidence="5">
        <location>
            <begin position="153"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="OmpR/PhoB-type" evidence="3">
        <location>
            <begin position="153"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="2">
        <location>
            <position position="74"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABF08059.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="266" mass="29964" checksum="1894FFF5073168D0" modified="2006-05-30" version="1">MADFCTIEPVQNRGPSRRTMEHAKRILLVEDDVGIANVLAMHLRDERYEVVHSADGAEGLRLLEQGGWDALVLDLMLPGVDGLEICRRARAMTRYTPIIITSARSSEVHRILGLELGADDYLAKPFSVLELVARVKALLRRVEAMARDVRADAGSLELAGLAIDPLAREAAVDGKRLDLTPREFDLLYYFARHPDKVFSRMDLLNAVWGYQHEGYEHTVNTHINRLRTKIEVDPAQPKRILTVWRRGYRFVADPQADAAQDEGEAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LPY4</accession>
    <name>Q1LPY4_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Putative acetyltransferase and hydrolase with the alpha/beta hydrolase fold</fullName>
            <ecNumber evidence="4">3.1.-.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Rmet_0906</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EC" id="3.1.-.-" evidence="4"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07792.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515732.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0906"/>
    <dbReference type="ESTHER" id="ralme-q1lpy4">
        <property type="family name" value="PGAP1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF07792">
        <property type="protein sequence ID" value="ABF07792"/>
        <property type="gene ID" value="Rmet_0906"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0906"/>
    <dbReference type="eggNOG" id="COG1075">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_074527_0_0_4"/>
    <dbReference type="OMA" id="LNYSPLT"/>
    <dbReference type="OrthoDB" id="1573721at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12697">
        <property type="entry name" value="Abhydrolase_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="SSF53474"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="38"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="domain" description="AB hydrolase-1" evidence="3">
        <location>
            <begin position="130"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="325"/>
            <end position="344"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF12697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF07792.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="344" mass="35944" checksum="9485E9A8AC0169CD" modified="2006-05-30" version="1">MSLTAAALRRIAVVVQAAAVGGLAAALAAGADWSWPAALLASIAGALVAFGIGVAIAFLVTRAGLAIPDSARPQRPDDIPAPGPLSWRHAMSCYLAECRAILRMFDWLQPFRSRLTFAQPTDPLPDTPTVLLVHGYGCNHAVWLDLAPALAGAGYRCEGIDLTPVLGDIDDYAAALLARMRDLRARTGHPPLLVCHSMGGLAARAAQVMADAAGESVPCAGIVTLGSPHRGCPLATFGAGTNARQMRWASPWLTNLAEAESPAMRGRMISVFSWHDSIAGPADTAWLEGARHIPLSGIGHVSLLRDPRAVQATLDGLATLRKQPAATSDSALSLPHGNASARFM</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-10-07" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LQ16</accession>
    <name>Q1LQ16_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_0874</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07760.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF07760">
        <property type="protein sequence ID" value="ABF07760"/>
        <property type="gene ID" value="Rmet_0874"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0874"/>
    <dbReference type="HOGENOM" id="CLU_1676419_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF07760.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="157" mass="17262" checksum="903EBED4202BFF10" modified="2006-05-30" version="1">MPFLLSSGAGDRSRTYDLRITNALLYQLSYTGETLNLRNLFYRLVSICAVACESIESDRARNNISDFLFCEAPGATFFNVPPWQSLFGGCCQPRPWATDGNHRTSGALVAGEKTGKACRFFTDCRAAVGFSKADRAPRRPVLVETGSTRYALAASSW</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="96" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LQ32</accession>
    <name>Q1LQ32_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Bifunctional (P)ppGpp synthetase II and guanosine-3',5'-bis pyrophosphate 3'-pyrophosphohydrolase</fullName>
            <ecNumber evidence="9">2.7.6.5</ecNumber>
            <ecNumber evidence="9">3.1.7.2</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">spoT</name>
        <name evidence="9" type="ordered locus">Rmet_0858</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">In eubacteria ppGpp (guanosine 3'-diphosphate 5'-diphosphate) is a mediator of the stringent response that coordinates a variety of cellular activities in response to changes in nutritional abundance.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the relA/spoT family.</text>
    </comment>
    <dbReference type="EC" id="2.7.6.5" evidence="9"/>
    <dbReference type="EC" id="3.1.7.2" evidence="9"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07744.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0858"/>
    <dbReference type="EnsemblBacteria" id="ABF07744">
        <property type="protein sequence ID" value="ABF07744"/>
        <property type="gene ID" value="Rmet_0858"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0858"/>
    <dbReference type="eggNOG" id="COG0317">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012300_3_0_4"/>
    <dbReference type="OMA" id="DWISSPK"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008728">
        <property type="term" value="F:GTP diphosphokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008893">
        <property type="term" value="F:guanosine-3',5'-bis(diphosphate) 3'-diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016779">
        <property type="term" value="F:nucleotidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015969">
        <property type="term" value="P:guanosine tetraphosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00077">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05399">
        <property type="entry name" value="NT_Rel-Spo_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01668">
        <property type="entry name" value="TGS_RSH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.460.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045865">
        <property type="entry name" value="ACT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002912">
        <property type="entry name" value="ACT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012675">
        <property type="entry name" value="Beta-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003607">
        <property type="entry name" value="HD/PDEase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006674">
        <property type="entry name" value="HD_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043519">
        <property type="entry name" value="NT_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004811">
        <property type="entry name" value="RelA/Spo_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045600">
        <property type="entry name" value="RelA/SpoT_AH_RIS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007685">
        <property type="entry name" value="RelA_SpoT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004095">
        <property type="entry name" value="TGS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012676">
        <property type="entry name" value="TGS-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033655">
        <property type="entry name" value="TGS_RelA/SpoT"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13291">
        <property type="entry name" value="ACT_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19296">
        <property type="entry name" value="DUF5913"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04607">
        <property type="entry name" value="RelA_SpoT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02824">
        <property type="entry name" value="TGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00471">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00954">
        <property type="entry name" value="RelA_SpoT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55021">
        <property type="entry name" value="SSF55021"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81271">
        <property type="entry name" value="SSF81271"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81301">
        <property type="entry name" value="SSF81301"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00691">
        <property type="entry name" value="spoT_relA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51671">
        <property type="entry name" value="ACT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51831">
        <property type="entry name" value="HD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51880">
        <property type="entry name" value="TGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="1" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="HD" evidence="7">
        <location>
            <begin position="197"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="domain" description="TGS" evidence="8">
        <location>
            <begin position="538"/>
            <end position="599"/>
        </location>
    </feature>
    <feature type="domain" description="ACT" evidence="6">
        <location>
            <begin position="795"/>
            <end position="872"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="24"/>
            <end position="74"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ABF07744.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="874" mass="96209" checksum="E35E354A4884A138" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LQE7</accession>
    <name>Q1LQE7_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Transcriptional regulator, LysR-family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_0743</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the LysR transcriptional regulatory family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07629.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515584.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0743"/>
    <dbReference type="EnsemblBacteria" id="ABF07629">
        <property type="protein sequence ID" value="ABF07629"/>
        <property type="gene ID" value="Rmet_0743"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0743"/>
    <dbReference type="eggNOG" id="COG0583">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039613_16_2_4"/>
    <dbReference type="OMA" id="MPHSLAR"/>
    <dbReference type="OrthoDB" id="1661500at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005119">
        <property type="entry name" value="LysR_subst-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000847">
        <property type="entry name" value="Tscrpt_reg_HTH_LysR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00126">
        <property type="entry name" value="HTH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03466">
        <property type="entry name" value="LysR_substrate"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50931">
        <property type="entry name" value="HTH_LYSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH lysR-type" evidence="2">
        <location>
            <begin position="3"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF07629.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="310" mass="33478" checksum="8827E1EA8CAE67FD" modified="2006-05-30" version="1">MPFDLTDIAVFVRAAALGNLSAAARDLGLSTSTASARLQRLEDRLGTRLLHRTTRRLSLTGDGERFLTHAEPLLAASEVAAQSVGRGAEVPHGPLRVTAPASFGRQHVSPAIPAFLATYPGITLDLRLSDQIVGLVDSGIDVALRMGALPDSSLVARPLAPSHRVICASPEYLARHGSPRHPEDLRNHNCLVLGDQSTWRFDTPVGESAVSVRGTLRADNGEVIRDAIVAGVGIALKSTWDVAPYLRRGELVTVLDEYPVLPVVSIWAVYPSRHLVPAKTHAFVDFFANWFGSPPYWDRDDAPPLATRTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LQH8</accession>
    <name>Q1LQH8_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Outer membrane protein or related peptidoglycan-associated (Lipo)protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">ompA</name>
        <name evidence="5" type="ordered locus">Rmet_0712</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07598.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008643648.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0712"/>
    <dbReference type="EnsemblBacteria" id="ABF07598">
        <property type="protein sequence ID" value="ABF07598"/>
        <property type="gene ID" value="Rmet_0712"/>
    </dbReference>
    <dbReference type="GeneID" id="60825199"/>
    <dbReference type="KEGG" id="rme:Rmet_0712"/>
    <dbReference type="eggNOG" id="COG2885">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016890_5_0_4"/>
    <dbReference type="OMA" id="CWRTGYW"/>
    <dbReference type="OrthoDB" id="2018797at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07185">
        <property type="entry name" value="OmpA_C-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006664">
        <property type="entry name" value="OMP_bac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006665">
        <property type="entry name" value="OmpA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036737">
        <property type="entry name" value="OmpA-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00691">
        <property type="entry name" value="OmpA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01021">
        <property type="entry name" value="OMPADOMAIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103088">
        <property type="entry name" value="SSF103088"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51123">
        <property type="entry name" value="OMPA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193635" evidence="3">
        <location>
            <begin position="23"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="domain" description="OmpA-like" evidence="4">
        <location>
            <begin position="89"/>
            <end position="214"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABF07598.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="217" mass="23298" checksum="4F97745A981DF7D6" modified="2006-05-30" version="1">MKKFAKLALVAATAVMAASAQAQSVPYEKKAVNDNWGNGTSEYVWKNGTNELCWRDSSWTPATANAQCDGALAPAAAAPAPTPVAPPVVSSEKVTFAADTLFDFDKAVLKPEGKAKLDDLVSKLQGITLEVIIAVGHTDSFGSDKYNDRLSIRRAESVKAYLVSKGVEANRVYTEGKGKRQLKVDPKSCKGNRTAQIACQQPNRRVEVEVVGTRSAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="109" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LQM4</accession>
    <name>Q1LQM4_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 9">4-hydroxy-tetrahydrodipicolinate synthase</fullName>
            <shortName evidence="9">HTPA synthase</shortName>
            <ecNumber evidence="4 9">4.3.3.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9 11" type="primary">dapA</name>
        <name evidence="11" type="ordered locus">Rmet_0666</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2 9">Catalyzes the condensation of (S)-aspartate-beta-semialdehyde [(S)-ASA] and pyruvate to 4-hydroxy-tetrahydrodipicolinate (HTPA).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 9">
            <text>L-aspartate 4-semialdehyde + pyruvate = (2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinate + H(+) + H2O</text>
            <dbReference type="Rhea" id="RHEA:34171"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:67139"/>
            <dbReference type="ChEBI" id="CHEBI:537519"/>
            <dbReference type="EC" id="4.3.3.7"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3 9">Amino-acid biosynthesis; L-lysine biosynthesis via DAP pathway; (S)-tetrahydrodipicolinate from L-aspartate: step 3/4.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Homotetramer; dimer of dimers.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9 10">Belongs to the DapA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">Was originally thought to be a dihydrodipicolinate synthase (DHDPS), catalyzing the condensation of (S)-aspartate-beta-semialdehyde [(S)-ASA] and pyruvate to dihydrodipicolinate (DHDP). However, it was shown in E.coli that the product of the enzymatic reaction is not dihydrodipicolinate but in fact (4S)-4-hydroxy-2,3,4,5-tetrahydro-(2S)-dipicolinic acid (HTPA), and that the consecutive dehydration reaction leading to DHDP is not spontaneous but catalyzed by DapB.</text>
    </comment>
    <dbReference type="EC" id="4.3.3.7" evidence="4 9"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07552.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515523.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0666"/>
    <dbReference type="EnsemblBacteria" id="ABF07552">
        <property type="protein sequence ID" value="ABF07552"/>
        <property type="gene ID" value="Rmet_0666"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0666"/>
    <dbReference type="eggNOG" id="COG0329">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_049343_7_1_4"/>
    <dbReference type="OMA" id="YYGYGGF"/>
    <dbReference type="OrthoDB" id="1438588at2"/>
    <dbReference type="UniPathway" id="UPA00034">
        <property type="reaction ID" value="UER00017"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008840">
        <property type="term" value="F:4-hydroxy-tetrahydrodipicolinate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019877">
        <property type="term" value="P:diaminopimelate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009089">
        <property type="term" value="P:lysine biosynthetic process via diaminopimelate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00950">
        <property type="entry name" value="DHDPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00418">
        <property type="entry name" value="DapA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005263">
        <property type="entry name" value="DapA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002220">
        <property type="entry name" value="DapA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020625">
        <property type="entry name" value="Schiff_base-form_aldolases_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12128">
        <property type="entry name" value="PTHR12128"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00701">
        <property type="entry name" value="DHDPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001365">
        <property type="entry name" value="DHDPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00146">
        <property type="entry name" value="DHPICSNTHASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM01130">
        <property type="entry name" value="DHDPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00674">
        <property type="entry name" value="dapA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00666">
        <property type="entry name" value="DHDPS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 9" id="KW-0220">Diaminopimelate biosynthesis</keyword>
    <keyword evidence="9 10 11" id="KW-0456">Lyase</keyword>
    <keyword evidence="7 9" id="KW-0457">Lysine biosynthesis</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 9" id="KW-0704">Schiff base</keyword>
    <feature type="active site" description="Proton donor/acceptor" evidence="9">
        <location>
            <position position="139"/>
        </location>
    </feature>
    <feature type="active site" description="Schiff-base intermediate with substrate" evidence="9">
        <location>
            <position position="167"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="52"/>
        </location>
        <ligand>
            <name>pyruvate</name>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="207"/>
        </location>
        <ligand>
            <name>pyruvate</name>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
        </ligand>
    </feature>
    <feature type="site" description="Part of a proton relay during catalysis" evidence="9">
        <location>
            <position position="51"/>
        </location>
    </feature>
    <feature type="site" description="Part of a proton relay during catalysis" evidence="9">
        <location>
            <position position="113"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00003294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022915"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRNR" id="PIRNR001365"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ABF07552.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="302" mass="32439" checksum="DF530C985D58F2D7" modified="2006-05-30" version="1">MQNTQSHPGFRGIWLPLVTPFHRGEVDFDALQRLVGHFSVTGIAGFVVCGSTGEAAALEEPEQLAVLDTVLECANGLPVIMGLAGNHLGHVLARLGRCNERPLAGVLAPAPYYIRPSQDGLRDYFRRLADASRAPLVLYDIPYRTGTTLMLETLLSLADHGNIGAIKDCGGNALNTQALIADGRLAVLAGEDHQLLGTLALGGQGAIIACAHFHPERFVAIWRAVSEGRLDEARRLFYALMPLIQTLFAEPNPGPIKALLARHGWLRDELRAPMTCASPELANQLAAMFDAMDIVSGAVSQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LQU9</accession>
    <name>Q1LQU9_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Putative signal transduction protein containing a membrane domain, an EAL and a GGDEF domain</fullName>
            <ecNumber evidence="11">2.7.3.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">Rmet_0591</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.7.3.-" evidence="11"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07477.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515451.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0591"/>
    <dbReference type="EnsemblBacteria" id="ABF07477">
        <property type="protein sequence ID" value="ABF07477"/>
        <property type="gene ID" value="Rmet_0591"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0591"/>
    <dbReference type="eggNOG" id="COG3447">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG3852">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG5001">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_126_2_4"/>
    <dbReference type="OMA" id="IVYTHMT"/>
    <dbReference type="OrthoDB" id="1635706at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01948">
        <property type="entry name" value="EAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01949">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00130">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.450">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001633">
        <property type="entry name" value="EAL_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035919">
        <property type="entry name" value="EAL_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000160">
        <property type="entry name" value="GGDEF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007895">
        <property type="entry name" value="MASE1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029787">
        <property type="entry name" value="Nucleotide_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001610">
        <property type="entry name" value="PAC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000014">
        <property type="entry name" value="PAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000700">
        <property type="entry name" value="PAS-assoc_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035965">
        <property type="entry name" value="PAS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013656">
        <property type="entry name" value="PAS_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013655">
        <property type="entry name" value="PAS_fold_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00563">
        <property type="entry name" value="EAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00990">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05231">
        <property type="entry name" value="MASE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08447">
        <property type="entry name" value="PAS_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08448">
        <property type="entry name" value="PAS_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00052">
        <property type="entry name" value="EAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00267">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00086">
        <property type="entry name" value="PAC"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00091">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF141868">
        <property type="entry name" value="SSF141868"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55073">
        <property type="entry name" value="SSF55073"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55785">
        <property type="entry name" value="SSF55785"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00254">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00229">
        <property type="entry name" value="sensory_box"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50883">
        <property type="entry name" value="EAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50887">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50113">
        <property type="entry name" value="PAC"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50112">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0808">Transferase</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="21"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="93"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="127"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="166"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="199"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="232"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="267"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="domain" description="PAS" evidence="7">
        <location>
            <begin position="303"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="domain" description="PAC" evidence="8">
        <location>
            <begin position="377"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="domain" description="PAC" evidence="8">
        <location>
            <begin position="506"/>
            <end position="558"/>
        </location>
    </feature>
    <feature type="domain" description="PAS" evidence="7">
        <location>
            <begin position="559"/>
            <end position="615"/>
        </location>
    </feature>
    <feature type="domain" description="PAC" evidence="8">
        <location>
            <begin position="636"/>
            <end position="689"/>
        </location>
    </feature>
    <feature type="domain" description="GGDEF" evidence="10">
        <location>
            <begin position="721"/>
            <end position="854"/>
        </location>
    </feature>
    <feature type="domain" description="EAL" evidence="9">
        <location>
            <begin position="865"/>
            <end position="1120"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ABF07477.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="1121" mass="123362" checksum="080243CB843843B1" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-12-02" version="65" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LRA7</accession>
    <name>Q1LRA7_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Nucleoside-diphosphate-sugar pyrophosphorylase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_0433</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07319.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0433"/>
    <dbReference type="EnsemblBacteria" id="ABF07319">
        <property type="protein sequence ID" value="ABF07319"/>
        <property type="gene ID" value="Rmet_0433"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0433"/>
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        <property type="taxonomic scope" value="Bacteria"/>
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    <dbReference type="HOGENOM" id="CLU_029499_2_1_4"/>
    <dbReference type="OMA" id="INHAHLG"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016779">
        <property type="term" value="F:nucleotidyltransferase activity"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009058">
        <property type="term" value="P:biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005835">
        <property type="entry name" value="NTP_transferase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00483">
        <property type="entry name" value="NTP_transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="SSF53448"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="NTP_transferase" evidence="2">
        <location>
            <begin position="1"/>
            <end position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF07319.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="232" mass="25086" checksum="1246E9124736792A" modified="2006-05-30" version="1">MIFAAGRGDRMRPLTDACPKPLLAVGGKPLIVWQVEALVRAGLTDIVINHAWLGEQLESALGDGSQFGARIHWSAEGTALETAGGIAHALPLLTGKTDADDVFLAVSGDIFCDFDYRTLLPRARAMAMAPRPHMHLVMVPNPPFHPRGDFVLDTAGLLRMPSADAHALTFGNIGLYDTRLFREIERGAKVAMSPYYRESIAAGLATGERFDGRWENVGTPAQLAALDRSLRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="64" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LRC7</accession>
    <name>Q1LRC7_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_0413</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07299.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515289.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0413"/>
    <dbReference type="EnsemblBacteria" id="ABF07299">
        <property type="protein sequence ID" value="ABF07299"/>
        <property type="gene ID" value="Rmet_0413"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0413"/>
    <dbReference type="eggNOG" id="COG1664">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_072799_7_0_4"/>
    <dbReference type="OMA" id="YAALEMH"/>
    <dbReference type="OrthoDB" id="1968497at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007607">
        <property type="entry name" value="BacA/B"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35024">
        <property type="entry name" value="PTHR35024"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04519">
        <property type="entry name" value="Bactofilin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="127"/>
            <end position="164"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF07299.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="164" mass="17015" checksum="E84AC942A24F899B" modified="2006-05-30" version="1">MLFSKKKGLSIDTLIGLGATIEGDVTFSGGLRLDGRVRGNVIAAEGKSSMLVISEKGLVEGEIRVDHLILNGTVTGPVHAAELLELQPHARVYGEVRYAALEMHQGAIVEGRLVPLTQTEVRALPNLAPSAEAKTDAPANDGEATASDQAADNDKAPEGAGVPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="108" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LRD9</accession>
    <name>Q1LRD9_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Glutamate and aspartate transporter subunit membrane component of ABC superfamily</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">gltK</name>
        <name evidence="9" type="ordered locus">Rmet_0401</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cell membrane</location>
            <topology evidence="7">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the binding-protein-dependent transport system permease family. HisMQ subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07287.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008645994.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0401"/>
    <dbReference type="EnsemblBacteria" id="ABF07287">
        <property type="protein sequence ID" value="ABF07287"/>
        <property type="gene ID" value="Rmet_0401"/>
    </dbReference>
    <dbReference type="GeneID" id="60824003"/>
    <dbReference type="KEGG" id="rme:Rmet_0401"/>
    <dbReference type="eggNOG" id="COG0765">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019602_1_1_4"/>
    <dbReference type="OMA" id="WQSMKLV"/>
    <dbReference type="OrthoDB" id="1523519at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043190">
        <property type="term" value="C:ATP-binding cassette (ABC) transporter complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006865">
        <property type="term" value="P:amino acid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010065">
        <property type="entry name" value="AA_ABC_transptr_permease_3TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043429">
        <property type="entry name" value="ArtM/GltK/GlnP/TcyL/YhdX-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030205">
        <property type="entry name" value="GltK/AatM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30614">
        <property type="entry name" value="PTHR30614"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30614:SF1">
        <property type="entry name" value="PTHR30614:SF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="SSF161098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01726">
        <property type="entry name" value="HEQRo_perm_3TM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0029">Amino-acid transport</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="7" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="22"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="64"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="97"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="200"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="8">
        <location>
            <begin position="22"/>
            <end position="218"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ABF07287.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="227" mass="25277" checksum="334D6CBF29AA4756" modified="2006-05-30" version="1">MAYEFDFSSINPSTLHVLGEGMLVSAKITVTAVIVGIVWGTILAMMRLSSNKLLNWFAQGYVTLFRSIPLVMVLLWFFLIIPQLLQKTFNLSAASDLRMTSALIAFSLFEAAYYSEIIRAGIQSVSRGQMFAAQAMGMTYGQSMRLVILPQAFRNMVPLLLTQGIILFQDTSLVYVSALADFFGQAYGIGERDGRIVEMLLFAGLIYFIICFSASLLVKRYQKKVAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LRN3</accession>
    <name>Q1LRN3_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">D-arabinose 5-phosphate isomerase</fullName>
            <ecNumber evidence="8">5.3.1.13</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">kdsD2</name>
        <name evidence="8" type="ordered locus">Rmet_0307</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the SIS family. GutQ/KpsF subfamily.</text>
    </comment>
    <dbReference type="EC" id="5.3.1.13" evidence="8"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07193.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515193.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0307"/>
    <dbReference type="EnsemblBacteria" id="ABF07193">
        <property type="protein sequence ID" value="ABF07193"/>
        <property type="gene ID" value="Rmet_0307"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0307"/>
    <dbReference type="eggNOG" id="COG0517">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG0794">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_040681_13_1_4"/>
    <dbReference type="OMA" id="LMACLMR"/>
    <dbReference type="OrthoDB" id="571638at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019146">
        <property type="term" value="F:arabinose-5-phosphate isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097367">
        <property type="term" value="F:carbohydrate derivative binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901135">
        <property type="term" value="P:carbohydrate derivative metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05014">
        <property type="entry name" value="SIS_Kpsf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.580.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004800">
        <property type="entry name" value="KdsD/KpsF-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001347">
        <property type="entry name" value="SIS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046348">
        <property type="entry name" value="SIS_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035474">
        <property type="entry name" value="SIS_Kpsf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00571">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01380">
        <property type="entry name" value="SIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004692">
        <property type="entry name" value="KdsD_KpsF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00116">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53697">
        <property type="entry name" value="SSF53697"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00393">
        <property type="entry name" value="kpsF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51371">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51464">
        <property type="entry name" value="SIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0129">CBS domain</keyword>
    <keyword evidence="8" id="KW-0413">Isomerase</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="SIS" evidence="7">
        <location>
            <begin position="39"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="6">
        <location>
            <begin position="208"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="6">
        <location>
            <begin position="275"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="80"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="site" description="Catalytically relevant" evidence="4">
        <location>
            <position position="57"/>
        </location>
    </feature>
    <feature type="site" description="Catalytically relevant" evidence="4">
        <location>
            <position position="109"/>
        </location>
    </feature>
    <feature type="site" description="Catalytically relevant" evidence="4">
        <location>
            <position position="150"/>
        </location>
    </feature>
    <feature type="site" description="Catalytically relevant" evidence="4">
        <location>
            <position position="191"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008165"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRNR" id="PIRNR004692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR004692-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR004692-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ABF07193.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="327" mass="34343" checksum="F19BA8C284A6F3D4" modified="2006-05-30" version="1">MIANFDADRALRLARQTLEIEAEAVSALAGRLTPAFTTAVEMILGCTGRVVVSGIGKSGHIGRKVAATLASTGTPSFFVHPAEASHGDLGMITRDDVLIAFSNSGETAELLAIIPIVKRIGAGLISITGNAESNLAKLSNAHLDGAVAQEACPLNLAPTASTTAALALGDALAVAVLDARGFGEDDFARSHPGGALGRKLLTHVRDVMRTGNAVPAVRESTPLAQALMEITRKGMAMTAVVDPDGRAVGVFTDGDLRRLLETPRDWKTVPIGEVMHRNPHTVHLDKLAVEAVQIMETNRINQLLVVDDDGHLAGALHIHDLTRAKVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LRR9</accession>
    <name>Q1LRR9_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Cytochrome oxidase assembly protein, Sco1/SenC family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">sco2</name>
        <name evidence="5" type="ordered locus">Rmet_0271</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SCO1/2 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07157.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515161.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0271"/>
    <dbReference type="EnsemblBacteria" id="ABF07157">
        <property type="protein sequence ID" value="ABF07157"/>
        <property type="gene ID" value="Rmet_0271"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0271"/>
    <dbReference type="eggNOG" id="COG1999">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_050131_3_0_4"/>
    <dbReference type="OMA" id="FELNGWM"/>
    <dbReference type="OrthoDB" id="1994201at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd02968">
        <property type="entry name" value="SCO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003782">
        <property type="entry name" value="SCO1/SenC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12151">
        <property type="entry name" value="PTHR12151"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02630">
        <property type="entry name" value="SCO1-SenC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="SSF52833"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0186">Copper</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193387" evidence="3">
        <location>
            <begin position="27"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="domain" description="Thioredoxin" evidence="4">
        <location>
            <begin position="26"/>
            <end position="204"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010996"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABF07157.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="204" mass="22583" checksum="8446DCE005DA3A03" modified="2006-05-30" version="1">MSTSSQGLWAAFRRFWLLALCTALLAACGQPKPSFRNVDISGATEFGKDFALLDPTGKTRTLADFKGKVVLMFFGYTHCPDVCPTTMVELKQVMEQMGPDADKVQVLFVTVDPDRDTAALMAQYVPAFDSRFLGLRPADEAALKKVTKDFKVYYAKVPGSSPDNYTMDHSAGSYVFDQNGQLRLFIRHGQGPEPIAHDLKQLMS</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-02-05" modified="2022-05-25" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LRT6</accession>
    <name>SECB_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Protein-export protein SecB</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">secB</name>
        <name type="ordered locus">Rmet_0254</name>
    </gene>
    <organism>
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">One of the proteins required for the normal export of preproteins out of the cell cytoplasm. It is a molecular chaperone that binds to a subset of precursor proteins, maintaining them in a translocation-competent state. It also specifically binds to its receptor SecA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homotetramer, a dimer of dimers. One homotetramer interacts with 1 SecA dimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the SecB family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07140.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008642655.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q1LRT6"/>
    <dbReference type="SMR" id="Q1LRT6"/>
    <dbReference type="STRING" id="266264.Rmet_0254"/>
    <dbReference type="EnsemblBacteria" id="ABF07140">
        <property type="protein sequence ID" value="ABF07140"/>
        <property type="gene ID" value="Rmet_0254"/>
    </dbReference>
    <dbReference type="GeneID" id="60822979"/>
    <dbReference type="KEGG" id="rme:Rmet_0254"/>
    <dbReference type="eggNOG" id="COG1952">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_111574_1_0_4"/>
    <dbReference type="OMA" id="CPNVLFP"/>
    <dbReference type="OrthoDB" id="1624074at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051262">
        <property type="term" value="P:protein tetramerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.420.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00821">
        <property type="entry name" value="SecB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003708">
        <property type="entry name" value="SecB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035958">
        <property type="entry name" value="SecB-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36918">
        <property type="entry name" value="PTHR36918"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02556">
        <property type="entry name" value="SecB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01594">
        <property type="entry name" value="SECBCHAPRONE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54611">
        <property type="entry name" value="SSF54611"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00809">
        <property type="entry name" value="secB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0143">Chaperone</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0653">Protein transport</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0811">Translocation</keyword>
    <keyword id="KW-0813">Transport</keyword>
    <feature type="chain" id="PRO_1000062501" description="Protein-export protein SecB">
        <location>
            <begin position="1"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="153"/>
            <end position="172"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="172" mass="18886" checksum="B6F9E62ECC4D3841" modified="2006-05-30" version="1">MSDQQQANQQDDQPFFNIQRVYLKDMSLEQPNSPGIFLESEAPSVEVQVNVGASQLQEGIFEVVVTGTVTTKVQDKVAFLVEAHQAGIFDIRNVPVEQLDPLLGIACPTILYPYLRGNIADVITRAGFQAIHLSEINFQALYEQRLQAAMEEAQGQGGDSGIVMPDGSQARH</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-10-31" modified="2022-05-25" version="65" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LS97</accession>
    <name>Y093_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">UPF0391 membrane protein Rmet_0093</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">Rmet_0093</name>
    </gene>
    <organism>
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0391 family.</text>
    </comment>
    <comment type="sequence caution" evidence="2">
        <conflict type="erroneous initiation">
            <sequence resource="EMBL-CDS" id="ABF06979" version="1"/>
        </conflict>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF06979.1"/>
        <property type="status" value="ALT_INIT"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q1LS97"/>
    <dbReference type="STRING" id="266264.Rmet_0093"/>
    <dbReference type="EnsemblBacteria" id="ABF06979">
        <property type="protein sequence ID" value="ABF06979"/>
        <property type="gene ID" value="Rmet_0093"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0093"/>
    <dbReference type="eggNOG" id="COG5487">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2344601_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01361">
        <property type="entry name" value="UPF0391"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009760">
        <property type="entry name" value="DUF1328"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07043">
        <property type="entry name" value="DUF1328"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036466">
        <property type="entry name" value="UCP036466"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000256767" description="UPF0391 membrane protein Rmet_0093">
        <location>
            <begin position="1"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="5"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="30"/>
            <end position="50"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="54" mass="5879" checksum="38DB75D5B702302C" modified="2006-10-31" version="2">MLYYALVFFIVALIAAIFGFGGIAAGAVEIAKILFLIFLVVAIVTFVMGLVRRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-04-26" modified="2022-01-19" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>Q58AD7</accession>
    <name>Q58AD7_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">CopB outer membrane protein involved in Cu(II)/Cu(I) resistance</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">copB1</name>
        <name evidence="3" type="ordered locus">Rmet_6113</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3 4">
        <name>pMOL30</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid pMOL30</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000354">
        <property type="protein sequence ID" value="ABF12972.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011229402.1">
        <property type="nucleotide sequence ID" value="NC_006466.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_145681.1">
        <property type="nucleotide sequence ID" value="NC_006466.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF12972">
        <property type="protein sequence ID" value="ABF12972"/>
        <property type="gene ID" value="Rmet_6113"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6113"/>
    <dbReference type="HOGENOM" id="CLU_042913_0_1_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid pMOL30"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009279">
        <property type="term" value="C:cell outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006878">
        <property type="term" value="P:cellular copper ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007939">
        <property type="entry name" value="Cu-R_B_prcur"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05275">
        <property type="entry name" value="CopB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5013531914" evidence="2">
        <location>
            <begin position="23"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="23"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="23"/>
            <end position="234"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF12972.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="495" mass="53535" checksum="AE3235F23CAC3D89" modified="2005-04-26" version="1">MYKYTRIAVAALLTAGMSAAWAQHSHSPNSSNNDGAPANEATPDSRPQKATAPTAKKQGSTPSAGQGSMQGMDHSKMQGMDQGSMQGMDHSKMQGMDQGSMQGMDHSKMQGMDQGSMQGMDHSKMQGMDQGSMQGMDQGSMQGMDHSKMQGMDQGSMQGMDHSKMQGMDQGSMQGMDHSKMQGMDQGSMQGMDHSKMQGMDQGSMQGMDHSKMQGMDQGSMQGMDHSKMQGMDQGSMQGMDHGAMQMQGGSAPPDARDPDAYSDGAKLGVGQYAIGPSRSLHMADEHIFASVLVDRLEWTKGNESNAASYEAQAWIGNAFNKFVIKAEGEVEKGKVPEARTELLWGHAIATYWDTQLGVRNDAGYGRPARNWLAFGVQGLAPYWFEVEATGYVGTEGRTALRLSGEYDLLLTQRLILQPRLEASLYGKNDPEIGIGSGLSSGAVGVRLRYEFSRQFAPYIGIERSQSFGNTANMVRASGGRSGETRFVAGIRMWF</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-04-26" modified="2022-01-19" version="103" xmlns="http://uniprot.org/uniprot">
    <accession>Q58AP4</accession>
    <name>Q58AP4_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Tyr recombinase activity site-specific recombination</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">int</name>
        <name evidence="7" type="ordered locus">Rmet_6006</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="7 8">
        <name>pMOL30</name>
    </geneLocation>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="8">Plasmid pMOL30</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000354">
        <property type="protein sequence ID" value="ABF12865.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011229325.1">
        <property type="nucleotide sequence ID" value="NC_007971.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_145571.1">
        <property type="nucleotide sequence ID" value="NC_006466.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF12865">
        <property type="protein sequence ID" value="ABF12865"/>
        <property type="gene ID" value="Rmet_6006"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6006"/>
    <dbReference type="HOGENOM" id="CLU_068392_0_0_4"/>
    <dbReference type="OMA" id="SEWEANP"/>
    <dbReference type="OrthoDB" id="1085477at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid pMOL30"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.443.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044068">
        <property type="entry name" value="CB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011010">
        <property type="entry name" value="DNA_brk_join_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013762">
        <property type="entry name" value="Integrase-like_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002104">
        <property type="entry name" value="Integrase_catalytic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010998">
        <property type="entry name" value="Integrase_recombinase_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00589">
        <property type="entry name" value="Phage_integrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56349">
        <property type="entry name" value="SSF56349"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51900">
        <property type="entry name" value="CB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51898">
        <property type="entry name" value="TYR_RECOMBINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0229">DNA integration</keyword>
    <keyword evidence="3" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="2 4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-0614">Plasmid</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Core-binding (CB)" evidence="6">
        <location>
            <begin position="16"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="domain" description="Tyr recombinase" evidence="5">
        <location>
            <begin position="136"/>
            <end position="391"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABF12865.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="391" mass="43483" checksum="1AD98022D9620E3D" modified="2005-04-26" version="1">MANKEIFEPKTVDWQNDPTQAFDGWLSTLKGRRKDAPQLKVSSVEIYKLQWGKFLAFLDQRKILVTETTAKDVSDFLSTLANTNASSQRERYRALLERVFKHLLGADATTDERLNPASCSSQGIEPGRKNLLNTALQMGFLRRSEYDILVSHLNSPLKPATDITAHRKQLRDRAMVAVFLGAGLKVHQALSLTVSCIETKRAVQITVQEEDSKFFHQVRVMPFAADLLKRWLAQRRKWERECLKKGRKVGDLVFPAEIVDPDRLQALEEDSPDAKPSAGSWIDLLDTEGAAGISRRGRNGTGTRLVGRAMNSVMALRVCEAVMQESGVYTSQEGAAAESISPQILRNSFAAALFASGETTLGVQNQLGFAEEISAARLKAAWDSWRSFGND</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-01-04" modified="2022-08-03" version="127" xmlns="http://uniprot.org/uniprot">
    <accession>Q5NUU5</accession>
    <name>Q5NUU5_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">ATPase (Type II general secretion pathway) Tn6049</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">gspA</name>
        <name evidence="2" type="ordered locus">Rmet_0320</name>
        <name evidence="3" type="ordered locus">Rmet_1999</name>
        <name evidence="4" type="ordered locus">Rmet_2009</name>
        <name evidence="5" type="ordered locus">Rmet_2406</name>
        <name evidence="6" type="ordered locus">Rmet_2551</name>
        <name evidence="7" type="ordered locus">Rmet_2836</name>
        <name evidence="8" type="ordered locus">Rmet_3141</name>
        <name evidence="9" type="ordered locus">Rmet_3930</name>
        <name evidence="10" type="ordered locus">Rmet_4423</name>
        <name evidence="11" type="ordered locus">Rmet_5479</name>
        <name evidence="12" type="ordered locus">Rmet_5942</name>
        <name evidence="13" type="ordered locus">Rmet_6336</name>
    </gene>
    <organism evidence="6 14">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="9">
        <name>megaplasmid</name>
    </geneLocation>
    <geneLocation type="plasmid" evidence="14">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <geneLocation type="plasmid" evidence="13 14">
        <name>pMOL28</name>
    </geneLocation>
    <geneLocation type="plasmid" evidence="12 14">
        <name>pMOL30</name>
    </geneLocation>
    <reference evidence="6" key="1">
        <citation type="submission" date="2006-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of the chromosome of Ralstonia metallidurans CH34.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Copeland A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Barry K."/>
                <person name="Detter J.C."/>
                <person name="Glavina del Rio T."/>
                <person name="Hammon N."/>
                <person name="Israni S."/>
                <person name="Dalin E."/>
                <person name="Tice H."/>
                <person name="Martinez M."/>
                <person name="Goltsman E."/>
                <person name="Pitluck S."/>
                <person name="Schmutz J."/>
                <person name="Larimer F."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Kim E."/>
                <person name="Mergeay M."/>
                <person name="Benotmane M.A."/>
                <person name="Vallaeys T."/>
                <person name="Michaux A."/>
                <person name="Monchy S."/>
                <person name="Dunn J."/>
                <person name="McCorkle S."/>
                <person name="Taghavi S."/>
                <person name="van der Lelie D."/>
                <person name="Richardson P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">CH34</strain>
            <plasmid evidence="9">megaplasmid</plasmid>
            <plasmid evidence="13">pMOL28</plasmid>
            <plasmid evidence="12">pMOL30</plasmid>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="14">Plasmid megaplasmid CH34</plasmid>
            <plasmid evidence="14">Plasmid pMOL28</plasmid>
            <plasmid evidence="14">Plasmid pMOL30</plasmid>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2010-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Benotmane R."/>
                <person name="Lapidus A."/>
                <person name="McCorkle S."/>
                <person name="Monchy S."/>
                <person name="Taghavi S."/>
                <person name="van der Lelie N."/>
                <person name="Dunn J."/>
                <person name="Leys N."/>
                <person name="Mergeay M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">CH34</strain>
            <plasmid evidence="9">megaplasmid</plasmid>
            <plasmid evidence="13">pMOL28</plasmid>
            <plasmid evidence="12">pMOL30</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07206.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08878.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08888.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09283.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09428.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09709.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10013.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10798.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11288.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12338.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP000354">
        <property type="protein sequence ID" value="ABF12801.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP000355">
        <property type="protein sequence ID" value="ABF13195.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008651548.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_145627.1">
        <property type="nucleotide sequence ID" value="NC_006466.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_161732.1">
        <property type="nucleotide sequence ID" value="NC_006525.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0320"/>
    <dbReference type="EnsemblBacteria" id="ABF07206">
        <property type="protein sequence ID" value="ABF07206"/>
        <property type="gene ID" value="Rmet_0320"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF08878">
        <property type="protein sequence ID" value="ABF08878"/>
        <property type="gene ID" value="Rmet_1999"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF08888">
        <property type="protein sequence ID" value="ABF08888"/>
        <property type="gene ID" value="Rmet_2009"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF09283">
        <property type="protein sequence ID" value="ABF09283"/>
        <property type="gene ID" value="Rmet_2406"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF09428">
        <property type="protein sequence ID" value="ABF09428"/>
        <property type="gene ID" value="Rmet_2551"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF09709">
        <property type="protein sequence ID" value="ABF09709"/>
        <property type="gene ID" value="Rmet_2836"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF10013">
        <property type="protein sequence ID" value="ABF10013"/>
        <property type="gene ID" value="Rmet_3141"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF10798">
        <property type="protein sequence ID" value="ABF10798"/>
        <property type="gene ID" value="Rmet_3930"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF11288">
        <property type="protein sequence ID" value="ABF11288"/>
        <property type="gene ID" value="Rmet_4423"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF12338">
        <property type="protein sequence ID" value="ABF12338"/>
        <property type="gene ID" value="Rmet_5479"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF12801">
        <property type="protein sequence ID" value="ABF12801"/>
        <property type="gene ID" value="Rmet_5942"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF13195">
        <property type="protein sequence ID" value="ABF13195"/>
        <property type="gene ID" value="Rmet_6336"/>
    </dbReference>
    <dbReference type="GeneID" id="60824629"/>
    <dbReference type="KEGG" id="rme:Rmet_0320"/>
    <dbReference type="KEGG" id="rme:Rmet_1999"/>
    <dbReference type="KEGG" id="rme:Rmet_2009"/>
    <dbReference type="KEGG" id="rme:Rmet_2406"/>
    <dbReference type="KEGG" id="rme:Rmet_2551"/>
    <dbReference type="KEGG" id="rme:Rmet_2836"/>
    <dbReference type="KEGG" id="rme:Rmet_3141"/>
    <dbReference type="KEGG" id="rme:Rmet_3930"/>
    <dbReference type="KEGG" id="rme:Rmet_4423"/>
    <dbReference type="KEGG" id="rme:Rmet_5479"/>
    <dbReference type="KEGG" id="rme:Rmet_5942"/>
    <dbReference type="KEGG" id="rme:Rmet_6336"/>
    <dbReference type="eggNOG" id="COG3267">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024125_1_0_4"/>
    <dbReference type="OMA" id="YHLGPLD"/>
    <dbReference type="OrthoDB" id="947558at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid pMOL28"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid pMOL30"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13401">
        <property type="entry name" value="AAA_22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-0614">Plasmid</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA" evidence="1">
        <location>
            <begin position="46"/>
            <end position="199"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF07206.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF08878.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF08888.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABF09283.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABF09428.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABF09709.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ABF10013.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ABF10798.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ABF11288.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ABF12338.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ABF12801.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ABF13195.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="277" mass="31235" checksum="EB30987461AADE43" modified="2005-01-04" version="1">MNQPNLLALYGLKFNPFAQDIPVEAVFVPPKLDHFCWRIENGMAREGGFAMVHGDPGCGKSVTLRLLHQRLMRVPDLMVGSIAHPQSNLADFYRELSDIFTVPLKPHNRWGGFKALRERWLTHMEASRRRCVLLIDEAQEMAVPALSELRLLAQARFDSQLLLCVVLAGDARLPEKFSREDLIPLGSRIRCRLALESASIDELQACLDHLLAAAGNATLMTMPLRQTLCEHAAGNYRILMNLAGELLAQAAQRELPQIDEKLYLETYSATLNRRARR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-01-04" modified="2021-09-29" version="92" xmlns="http://uniprot.org/uniprot">
    <accession>Q5NUY7</accession>
    <name>Q5NUY7_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">ATPase involved in plasmid partitioning ParA</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">parA</name>
        <name evidence="2" type="ordered locus">Rmet_6315</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2 3">
        <name>pMOL28</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid pMOL28</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000355">
        <property type="protein sequence ID" value="ABF13174.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008649475.1">
        <property type="nucleotide sequence ID" value="NC_007972.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_161627.1">
        <property type="nucleotide sequence ID" value="NC_006525.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF13174">
        <property type="protein sequence ID" value="ABF13174"/>
        <property type="gene ID" value="Rmet_6315"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6315"/>
    <dbReference type="HOGENOM" id="CLU_037612_9_0_4"/>
    <dbReference type="OMA" id="HPIEMTR"/>
    <dbReference type="OrthoDB" id="729012at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid pMOL28"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025669">
        <property type="entry name" value="AAA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13614">
        <property type="entry name" value="AAA_31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA_31" evidence="1">
        <location>
            <begin position="107"/>
            <end position="290"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF13174.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="396" mass="44396" checksum="E8CDEAEF26662C10" modified="2005-01-04" version="1">MDIKLAHPSKTTPEDLKQLANLSAVMLQKIRDEMLEPFPRKEAPLIPSGRLQELCGIDKTRMNRSLKKGDLPQGQQSRPGAVRYFSLSEAMQWIRAELKPVPRRGPGKVIAVANFKGGVTKTTMSTLLCQGLSLRRGRKVCHVDLDPQGSATTLYGINPHAEVSSENTIMPLIEAYLAGESFDMRGLPQETYWPNLDLIPSSTELFNAEFMLPARATAEEGHIPFERVLSNGLDSLKDEYDYIILDTAPTLSYLTINAIFAADGVIVPVVPDTLAFASMVQFWQLFSDLVTGMEEQSEGSKKEFDFLDVLMTRMEKKNAPRLVADWIRGVYGSRVLPIEIPETDLARNSSIQFRTVYDLSSSEANTETMRRIRQPCDEFVDYVDDKVSALWQGIEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-01-04" modified="2021-09-29" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>Q5NV06</accession>
    <name>Q5NV06_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Type II secretory pathway, component PulD</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">pilN</name>
        <name evidence="2" type="ordered locus">Rmet_6294</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2 3">
        <name>pMOL28</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid pMOL28</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000355">
        <property type="protein sequence ID" value="ABF13153.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011239906.1">
        <property type="nucleotide sequence ID" value="NC_007972.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_161646.1">
        <property type="nucleotide sequence ID" value="NC_006525.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF13153">
        <property type="protein sequence ID" value="ABF13153"/>
        <property type="gene ID" value="Rmet_6294"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6294"/>
    <dbReference type="HOGENOM" id="CLU_015882_1_0_4"/>
    <dbReference type="OMA" id="KPWVNLK"/>
    <dbReference type="OrthoDB" id="1452829at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid pMOL28"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="258"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="258"/>
            <end position="279"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF13153.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="600" mass="63189" checksum="8ED2A49F25B92A5B" modified="2005-01-04" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2005-01-04" modified="2021-09-29" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>Q5NV37</accession>
    <name>Q5NV37_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Lipoprotein transmembrane</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_6287</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3 4">
        <name>pMOL28</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid pMOL28</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000355">
        <property type="protein sequence ID" value="ABF13146.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011239913.1">
        <property type="nucleotide sequence ID" value="NC_007972.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_161653.1">
        <property type="nucleotide sequence ID" value="NC_006525.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF13146">
        <property type="protein sequence ID" value="ABF13146"/>
        <property type="gene ID" value="Rmet_6287"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6287"/>
    <dbReference type="HOGENOM" id="CLU_080659_0_0_4"/>
    <dbReference type="OMA" id="HYAVGTP"/>
    <dbReference type="OrthoDB" id="1941395at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid pMOL28"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5013532128" evidence="2">
        <location>
            <begin position="24"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="28"/>
            <end position="47"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF13146.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="273" mass="27353" checksum="4204FFF05039A8B1" modified="2005-01-04" version="1">MQKTKIALSIVSAAALLTLAACGGGGGDSAAVTQQPATPTPPTPSITVQGSAAAVQADPLAVVYVPHTETSTRPAFQSISHAAFPAQYQKATQSGAYTQVGIDANSLITLTSGKVVDVTGNGDYAIGRWTDGSSTIGAVNVNQGDHYAVGTPLKLLQVLGIGKTLACTQIASTSPTAVSGNFPVGKLNSATAVIDLNGPTLQTLNLDVAIGSDAHATANIVGTVLNGVTQSNGVLHHVQTLGTSQSQPLLAIGYAMPTPSSGDVTGVVILKCQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-03-23" modified="2020-10-07" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>D3DY82</accession>
    <name>D3DY82_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_6653</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ADC45252.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADC45252">
        <property type="protein sequence ID" value="ADC45252"/>
        <property type="gene ID" value="Rmet_6653"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6653"/>
    <dbReference type="HOGENOM" id="CLU_3083913_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADC45252.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="52" mass="5680" checksum="C49122FB01B6855D" modified="2010-03-23" version="1">MFASGNTGFTNCLIDRAAHAEGCEYVATFDRAAGHMPGFRDIGRHPYPLSLH</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-03-23" modified="2020-02-26" version="16" xmlns="http://uniprot.org/uniprot">
    <accession>D3DYE7</accession>
    <name>D3DYE7_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_6738</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ADC45317.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADC45317">
        <property type="protein sequence ID" value="ADC45317"/>
        <property type="gene ID" value="Rmet_6738"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6738"/>
    <dbReference type="HOGENOM" id="CLU_2900991_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADC45317.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="62" mass="7012" checksum="0EF038104DBC8590" modified="2010-03-23" version="1">MLEKPYRGITPKRVDDGSVALSFLEQSTAQRLVGQPHRSSKLRLGREMRDIPLNKAFSNLDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-03-23" modified="2020-10-07" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>D3DYJ2</accession>
    <name>D3DYJ2_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_6387</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="1 2">
        <name>pMOL30</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid pMOL30</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000354">
        <property type="protein sequence ID" value="ADC45362.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADC45362">
        <property type="protein sequence ID" value="ADC45362"/>
        <property type="gene ID" value="Rmet_6387"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6387"/>
    <dbReference type="HOGENOM" id="CLU_2828129_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid pMOL30"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADC45362.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="66" mass="7382" checksum="29F20E60918DB0AA" modified="2010-03-23" version="1">MPLPPEVQMFVNIARERDRADLLFPSVDVPGPLGSLIRQPNPAAVRAREMKNLTDEEYAVRLGLLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="54" xmlns="http://uniprot.org/uniprot">
    <accession>Q1L9N7</accession>
    <name>Q1L9N7_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_6280</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="1 2">
        <name>pMOL28</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid pMOL28</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000355">
        <property type="protein sequence ID" value="ABF13139.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008649407.1">
        <property type="nucleotide sequence ID" value="NC_007972.2"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF13139">
        <property type="protein sequence ID" value="ABF13139"/>
        <property type="gene ID" value="Rmet_6280"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6280"/>
    <dbReference type="HOGENOM" id="CLU_1812557_0_0_4"/>
    <dbReference type="OMA" id="GWIMELW"/>
    <dbReference type="OrthoDB" id="1714285at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid pMOL28"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF13139.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="143" mass="15922" checksum="EC01C14404FA8FB8" modified="2006-05-30" version="1">METTAVGWQLDFFGEAAAIVVPPPKVDPLPDPSLWSESVREQMIDALISLACDSRRGDNMPESLVDCADLLRDRIRNKSSLDLEDYSMTLGWIFGYWDGALPYTYVCAVCGVSPEVLQDVILNHARLKADLDEVRRQCCGSLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LA53</accession>
    <name>Q1LA53_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">CopC Copper resistance protein, ''E set domains'' superfamily CopC Cu(II) binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">copC1</name>
        <name evidence="6" type="ordered locus">Rmet_6114</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="6 7">
        <name>pMOL30</name>
    </geneLocation>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="7">Plasmid pMOL30</plasmid>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CopC family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000354">
        <property type="protein sequence ID" value="ABF12973.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF12973">
        <property type="protein sequence ID" value="ABF12973"/>
        <property type="gene ID" value="Rmet_6114"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6114"/>
    <dbReference type="HOGENOM" id="CLU_087859_4_2_4"/>
    <dbReference type="OMA" id="TEMPGMA"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid pMOL30"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042597">
        <property type="term" value="C:periplasmic space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046688">
        <property type="term" value="P:response to copper ion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1220">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007348">
        <property type="entry name" value="CopC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014755">
        <property type="entry name" value="Cu-Rt/internalin_Ig-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04234">
        <property type="entry name" value="CopC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="SSF81296"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0186">Copper</keyword>
    <keyword evidence="6" id="KW-0614">Plasmid</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004192993" evidence="4">
        <location>
            <begin position="30"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="domain" description="CopC" evidence="5">
        <location>
            <begin position="30"/>
            <end position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010509"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF04234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABF12973.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="132" mass="14058" checksum="1E774FD5045A6948" modified="2006-05-30" version="1">MEFFMQASRLTIRATLAAAALLVSSVAFAHPKLLTSTPADKAEVAAPQKIELKFSENLSTQFSGANLLMTGMPGMADHSPMKIAAKVAGGDDPKTMVVTPTQPLSPGNYRVEWRAVSSDTHPINGNITFTVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-02-26" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LA58</accession>
    <name>Q1LA58_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">CopN involved in Cu(II)/Cu(I) resistance</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">copN</name>
        <name evidence="1" type="ordered locus">Rmet_6109</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="1 2">
        <name>pMOL30</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid pMOL30</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000354">
        <property type="protein sequence ID" value="ABF12968.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF12968">
        <property type="protein sequence ID" value="ABF12968"/>
        <property type="gene ID" value="Rmet_6109"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6109"/>
    <dbReference type="HOGENOM" id="CLU_1617641_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid pMOL30"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF12968.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="164" mass="17902" checksum="06B9FCB840CE07C2" modified="2006-05-30" version="1">MLVSLRREAEASDPMGFQILLQWRQALRRIRYGIVGLASLSGLVAHAHIIPIPDVQPAAAAYLAPGASGKTWPATEWVVEVDRLDGQPASRAVRTGESVSLSLRNLGEKSIDVAVVLSQSEAMTPMANSQPVTIHLLAGERQEWRMQATRPLRIFVSASPEWAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LAK1</accession>
    <name>Q1LAK1_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Glycosyltransferase (Protein o-glycosylation), family 39</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">gtrM1</name>
        <name evidence="11" type="ordered locus">Rmet_5966</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="11 12">
        <name>pMOL30</name>
    </geneLocation>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="12">Plasmid pMOL30</plasmid>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP000354">
        <property type="protein sequence ID" value="ABF12825.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008650720.1">
        <property type="nucleotide sequence ID" value="NC_007971.2"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF12825">
        <property type="protein sequence ID" value="ABF12825"/>
        <property type="gene ID" value="Rmet_5966"/>
    </dbReference>
    <dbReference type="GeneID" id="60820432"/>
    <dbReference type="KEGG" id="rme:Rmet_5966"/>
    <dbReference type="HOGENOM" id="CLU_019200_0_0_4"/>
    <dbReference type="OMA" id="DWITPWY"/>
    <dbReference type="OrthoDB" id="854536at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid pMOL30"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040845">
        <property type="entry name" value="Arnt_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038731">
        <property type="entry name" value="RgtA/B/C-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18583">
        <property type="entry name" value="Arnt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13231">
        <property type="entry name" value="PMT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="3" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-0614">Plasmid</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="77"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="103"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="127"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="158"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="198"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="247"/>
            <end position="270"/>
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        <location>
            <begin position="282"/>
            <end position="300"/>
        </location>
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    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="335"/>
            <end position="353"/>
        </location>
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    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="373"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="399"/>
            <end position="419"/>
        </location>
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        <location>
            <begin position="54"/>
            <end position="213"/>
        </location>
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        <location>
            <begin position="436"/>
            <end position="534"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF13231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF18583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ABF12825.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="535" mass="58550" checksum="74187D9AACF60FBE" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-12-02" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LAR9</accession>
    <name>Q1LAR9_CUPMC</name>
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        <recommendedName>
            <fullName evidence="1">LUD_dom domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_5898</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12757.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5898"/>
    <dbReference type="EnsemblBacteria" id="ABF12757">
        <property type="protein sequence ID" value="ABF12757"/>
        <property type="gene ID" value="Rmet_5898"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5898"/>
    <dbReference type="eggNOG" id="COG1556">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_090664_1_2_4"/>
    <dbReference type="OMA" id="MEAVHTE"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10420">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024185">
        <property type="entry name" value="FTHF_cligase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003741">
        <property type="entry name" value="LUD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037171">
        <property type="entry name" value="NagB/RpiA_transferase-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02589">
        <property type="entry name" value="LUD_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100950">
        <property type="entry name" value="SSF100950"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="LUD_dom" evidence="1">
        <location>
            <begin position="57"/>
            <end position="229"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02589"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF12757.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="236" mass="25202" checksum="DCDC90D1B838E739" modified="2006-05-30" version="1">MTTMSARERMIGRLRAAAPASGATLSGDTQKLDRRIDDHFDSRRGQAPSIDAMVASMQAALTASHADVICTDAASWPAWVARRLADEGVRRLLLDRDRPEGAALARALPSSIDTVTFDRPLEAWKAELFDTVDAGFTVARSGLAATGTLVLAPDAGSPRTMSLVPPTHIALVYASALHPDLHAAARAERWLDGMPTNLVMVSGPSKTSDIQQTLAYGAHGPRRLWVVIVTDGEAAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="102" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LB25</accession>
    <name>Q1LB25_CUPMC</name>
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        <submittedName>
            <fullName evidence="8">Cytochrome o ubiquinol oxidase subunit I</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">cyoB</name>
        <name evidence="8" type="ordered locus">Rmet_5792</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
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        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="9">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="9">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the heme-copper respiratory oxidase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12651.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011520188.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5792"/>
    <dbReference type="EnsemblBacteria" id="ABF12651">
        <property type="protein sequence ID" value="ABF12651"/>
        <property type="gene ID" value="Rmet_5792"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5792"/>
    <dbReference type="eggNOG" id="COG0843">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011899_7_1_4"/>
    <dbReference type="OMA" id="FFVWLHH"/>
    <dbReference type="OrthoDB" id="316745at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
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        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070469">
        <property type="term" value="C:respirasome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004129">
        <property type="term" value="F:cytochrome-c oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016682">
        <property type="term" value="F:oxidoreductase activity, acting on diphenols and related substances as donors, oxygen as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009060">
        <property type="term" value="P:aerobic respiration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023616">
        <property type="entry name" value="Cyt_c_oxase-like_su1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036927">
        <property type="entry name" value="Cyt_c_oxase-like_su1_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000883">
        <property type="entry name" value="Cyt_C_Oxase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023615">
        <property type="entry name" value="Cyt_c_Oxase_su1_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014207">
        <property type="entry name" value="Cyt_c_ubiqinol_oxidase_su1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10422">
        <property type="entry name" value="PTHR10422"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00115">
        <property type="entry name" value="COX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01165">
        <property type="entry name" value="CYCOXIDASEI"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81442">
        <property type="entry name" value="SSF81442"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02843">
        <property type="entry name" value="CyoB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50855">
        <property type="entry name" value="COX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00077">
        <property type="entry name" value="COX1_CUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0249">Electron transport</keyword>
    <keyword evidence="5" id="KW-0349">Heme</keyword>
    <keyword evidence="5" id="KW-0408">Iron</keyword>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0614">Plasmid</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0679">Respiratory chain</keyword>
    <keyword evidence="2 5 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="16"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="57"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="103"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="146"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="190"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="227"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="287"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="317"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="342"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="382"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="415"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="451"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="494"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="589"/>
            <end position="607"/>
        </location>
    </feature>
    <feature type="domain" description="COX1" evidence="7">
        <location>
            <begin position="39"/>
            <end position="559"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU000370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50855"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ABF12651.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="658" mass="73972" checksum="F5B5DD8790A40C7A" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LBS4</accession>
    <name>Q1LBS4_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">L-carnitine dehydratase/bile acid-inducible protein F</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">caiB</name>
        <name evidence="1" type="ordered locus">Rmet_5543</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12402.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519946.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5543"/>
    <dbReference type="EnsemblBacteria" id="ABF12402">
        <property type="protein sequence ID" value="ABF12402"/>
        <property type="gene ID" value="Rmet_5543"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5543"/>
    <dbReference type="eggNOG" id="COG1804">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033975_0_0_4"/>
    <dbReference type="OrthoDB" id="969868at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1540.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10540">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003673">
        <property type="entry name" value="CoA-Trfase_fam_III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044855">
        <property type="entry name" value="CoA-Trfase_III_dom3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023606">
        <property type="entry name" value="CoA-Trfase_III_dom_1_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02515">
        <property type="entry name" value="CoA_transf_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89796">
        <property type="entry name" value="SSF89796"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF12402.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="421" mass="45747" checksum="76EFF492C7D85011" modified="2006-05-30" version="1">MFDQARVESLPEHEAGGPKSGGVLDGVRVIDFTHFVAGPLATMTMADFGADVIKVEAPVKGDDFRYYPPMDPEIPAQGAPYLWTNRNKRSIGIDMKSPEGLALAKSLIAEADVVVENFSTGVMERFGLDYETCRKLNPRLIYCSVSAYGRTGPYADRLGFDPIAQAESGFISMNGYSDRQGVRTGAAVMDISTAMMATNAILLALLGRAKDGEGQFVEVALFDTAILMTGFAAMQHLTTGYEPQRNGNTSPDTCPSGVFHCQDVPFYINCGNDGIFVRLFEQVLDRPDIAHDPVLSKRANRLERRDELFKLMGEEFGKHPWAYWQPKLRAAGVPSGQVKTLAQALSSDEAASRNLVSRIQHPVKGWIPNISTPLRLSRTPARAATAAPAVGADTEQVLKDVLKLSDRQISELTASGAFGAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LC65</accession>
    <name>Q1LC65_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Putative alkyl sulfatase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Rmet_5402</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="8">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="8">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the metallo-beta-lactamase superfamily. Type III sulfatase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12261.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519809.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF12261">
        <property type="protein sequence ID" value="ABF12261"/>
        <property type="gene ID" value="Rmet_5402"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5402"/>
    <dbReference type="eggNOG" id="COG2015">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014655_1_0_4"/>
    <dbReference type="OMA" id="VYMYGES"/>
    <dbReference type="OrthoDB" id="775108at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018741">
        <property type="term" value="F:alkyl sulfatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018909">
        <property type="term" value="P:dodecyl sulfate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07710">
        <property type="entry name" value="arylsulfatase_Sdsa1-like_MBL-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1050.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038536">
        <property type="entry name" value="Alkyl/aryl-sulf_dimr_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029229">
        <property type="entry name" value="Alkyl_sulf_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029228">
        <property type="entry name" value="Alkyl_sulf_dimr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044097">
        <property type="entry name" value="Bds1/SdsA1_MBL-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001279">
        <property type="entry name" value="Metallo-B-lactamas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036527">
        <property type="entry name" value="SCP2_sterol-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14864">
        <property type="entry name" value="Alkyl_sulf_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14863">
        <property type="entry name" value="Alkyl_sulf_dimr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00753">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00849">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55718">
        <property type="entry name" value="SSF55718"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="SSF56281"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0614">Plasmid</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193314" evidence="5">
        <location>
            <begin position="28"/>
            <end position="660"/>
        </location>
    </feature>
    <feature type="domain" description="Lactamase_B" evidence="6">
        <location>
            <begin position="131"/>
            <end position="353"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00033751"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABF12261.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="660" mass="72390" checksum="1CB7880FBD480485" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LC72</accession>
    <name>Q1LC72_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Hydrolase_4 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_5395</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12254.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5395"/>
    <dbReference type="EnsemblBacteria" id="ABF12254">
        <property type="protein sequence ID" value="ABF12254"/>
        <property type="gene ID" value="Rmet_5395"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5395"/>
    <dbReference type="eggNOG" id="COG2267">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_043381_0_0_4"/>
    <dbReference type="OMA" id="MYSLSHV"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022742">
        <property type="entry name" value="Hydrolase_4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12146">
        <property type="entry name" value="Hydrolase_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="SSF53474"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Hydrolase_4" evidence="2">
        <location>
            <begin position="125"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="495"/>
            <end position="528"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF12254.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="541" mass="59377" checksum="D4C4F85BB7867861" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="106" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LC74</accession>
    <name>Q1LC74_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Histidine kinase</fullName>
            <ecNumber evidence="2">2.7.13.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">Rmet_5393</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="10">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="10">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.</text>
            <dbReference type="EC" id="2.7.13.3"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.7.13.3" evidence="2"/>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12252.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519798.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5393"/>
    <dbReference type="EnsemblBacteria" id="ABF12252">
        <property type="protein sequence ID" value="ABF12252"/>
        <property type="gene ID" value="Rmet_5393"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5393"/>
    <dbReference type="eggNOG" id="COG0642">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_89_37_4"/>
    <dbReference type="OMA" id="QLISVFW"/>
    <dbReference type="OrthoDB" id="692375at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00082">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003660">
        <property type="entry name" value="HAMP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003661">
        <property type="entry name" value="HisK_dim/P"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036097">
        <property type="entry name" value="HisK_dim/P_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00512">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00388">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47384">
        <property type="entry name" value="SSF47384"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="SSF55874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50885">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 9" id="KW-0418">Kinase</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9" id="KW-0614">Plasmid</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="149"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="domain" description="HAMP" evidence="8">
        <location>
            <begin position="175"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="7">
        <location>
            <begin position="235"/>
            <end position="426"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ABF12252.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="450" mass="48222" checksum="EE1DBF6A5D742966" modified="2006-05-30" version="1">MLRSLQGRLSAALVAGLVVLACAAAAASIHVLRQEVDRMSDSALQEAAQRLLPLALTEIMRREADDDEDDSSRVTAVRPHDELLTYVVRDDKGSLLLQSHDADLSVFPAGLKPGFSSTLSHRIYTEPGLQGTVALTIAEPLAARRQAKVAAALSVVRVLILFLPVGLIGIWLIVRTGLQPIRRFCGSIAARGQSDLTSLEKSGLPTEIAPVADAVNGLMARLDRALAAERSFTANSAHELRTPIAAALAQTQRLKADMPTDDMRERVQNVEHSLRTLARLSESLMQLAKAENGRVLGDATQDLRPVIELVIEDFRRIAHTPNPITLDLPDQPVESWIDPDAFAIVVRNLVDNAQKHGARGEPVRVSLSEAGAFTVANRGPVLAENDLARLMKPFERGATTARGSGLGLAIVEAIVRGAGATLTAHSPAPGWPDGLWITIDGLPLATMGPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LC97</accession>
    <name>Q1LC97_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Extra-cytoplasmic solute receptor protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">bug</name>
        <name evidence="4" type="ordered locus">Rmet_5370</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="5">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="5">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Periplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the UPF0065 (bug) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12229.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519776.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5370"/>
    <dbReference type="EnsemblBacteria" id="ABF12229">
        <property type="protein sequence ID" value="ABF12229"/>
        <property type="gene ID" value="Rmet_5370"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5370"/>
    <dbReference type="eggNOG" id="COG3181">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045683_0_0_4"/>
    <dbReference type="OMA" id="NCASTGV"/>
    <dbReference type="OrthoDB" id="447734at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042597">
        <property type="term" value="C:periplasmic space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005064">
        <property type="entry name" value="BUG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042100">
        <property type="entry name" value="Bug_dom1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42928">
        <property type="entry name" value="PTHR42928"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03401">
        <property type="entry name" value="TctC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017082">
        <property type="entry name" value="YflP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-0675">Receptor</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004192998" evidence="3">
        <location>
            <begin position="23"/>
            <end position="322"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF12229.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="322" mass="33825" checksum="325FBDB0AC051A29" modified="2006-05-30" version="1">MKNFLRHAAVGLMLACAATGHAQQPYPSKPIRVVVAFTAGGTTDILAREVAQQMSKALGQSVVVENRPGAGGNIGTDFVGRSDADGYTLLATSVGPVAINPTLYRKLKVNPQTDLQAVAPIADVPNVLVVYPGLPVKSVQDLVAYAKTHPNGLNFGSTGVGTAAHLSGVLFNERTGAKTTHVPYKGADALNDLLAGRVQFMFATIPSVISHIRGGQLRAIAVTSRKRSSALPDVPTMAEAGVPNLVTGAWFGLFAPKGTPPEIVVRLNKIVTDALSQPEMRGRFAQQGAESMNMTADAFGKFVAAEYLTWKPVVISSGARID</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="118" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LCH1</accession>
    <name>Q1LCH1_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7 19">Aconitate hydratase B</fullName>
            <ecNumber evidence="5 19">4.2.1.3</ecNumber>
            <ecNumber evidence="6 19">4.2.1.99</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="19">2-methylisocitrate dehydratase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="25" type="primary">acnB</name>
        <name evidence="25" type="ordered locus">Rmet_5296</name>
    </gene>
    <organism evidence="25 26">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="26">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="26" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="26">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="26">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 19">
            <text>(2S,3R)-3-hydroxybutane-1,2,3-tricarboxylate = 2-methyl-cis-aconitate + H2O</text>
            <dbReference type="Rhea" id="RHEA:17941"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:57429"/>
            <dbReference type="ChEBI" id="CHEBI:57872"/>
            <dbReference type="EC" id="4.2.1.99"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18 19">
            <text>citrate = D-threo-isocitrate</text>
            <dbReference type="Rhea" id="RHEA:10336"/>
            <dbReference type="ChEBI" id="CHEBI:15562"/>
            <dbReference type="ChEBI" id="CHEBI:16947"/>
            <dbReference type="EC" id="4.2.1.3"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="20">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="20">Binds 1 [4Fe-4S] cluster per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 19">Carbohydrate metabolism; tricarboxylic acid cycle; isocitrate from oxaloacetate: step 2/2.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3">Organic acid metabolism; propanoate degradation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 19">Belongs to the aconitase/IPM isomerase family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.3" evidence="5 19"/>
    <dbReference type="EC" id="4.2.1.99" evidence="6 19"/>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12155.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519701.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5296"/>
    <dbReference type="EnsemblBacteria" id="ABF12155">
        <property type="protein sequence ID" value="ABF12155"/>
        <property type="gene ID" value="Rmet_5296"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5296"/>
    <dbReference type="eggNOG" id="COG1049">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016536_0_0_4"/>
    <dbReference type="OMA" id="QDTTGAM"/>
    <dbReference type="OrthoDB" id="32104at2"/>
    <dbReference type="UniPathway" id="UPA00223">
        <property type="reaction ID" value="UER00718"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00946"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047456">
        <property type="term" value="F:2-methylisocitrate dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003994">
        <property type="term" value="F:aconitate hydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047780">
        <property type="term" value="F:citrate dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009098">
        <property type="term" value="P:leucine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd01576">
        <property type="entry name" value="AcnB_Swivel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.310">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.19.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.499.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1060.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015931">
        <property type="entry name" value="Acnase/IPM_dHydase_lsu_aba_1/3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001030">
        <property type="entry name" value="Acoase/IPM_deHydtase_lsu_aba"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015928">
        <property type="entry name" value="Aconitase/3IPM_dehydase_swvl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018136">
        <property type="entry name" value="Aconitase_4Fe-4S_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036008">
        <property type="entry name" value="Aconitase_4Fe-4S_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004406">
        <property type="entry name" value="Aconitase_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015933">
        <property type="entry name" value="Aconitase_B_HEAT-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036288">
        <property type="entry name" value="Aconitase_B_HEAT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015929">
        <property type="entry name" value="Aconitase_B_swivel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015932">
        <property type="entry name" value="Aconitase_dom2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00330">
        <property type="entry name" value="Aconitase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06434">
        <property type="entry name" value="Aconitase_2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11791">
        <property type="entry name" value="Aconitase_B_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036687">
        <property type="entry name" value="AcnB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53732">
        <property type="entry name" value="SSF53732"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF74778">
        <property type="entry name" value="SSF74778"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00117">
        <property type="entry name" value="acnB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00450">
        <property type="entry name" value="ACONITASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01244">
        <property type="entry name" value="ACONITASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 20" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="11" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="17" id="KW-0100">Branched-chain amino acid biosynthesis</keyword>
    <keyword evidence="14 20" id="KW-0408">Iron</keyword>
    <keyword evidence="15 20" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="8" id="KW-0432">Leucine biosynthesis</keyword>
    <keyword evidence="16 19 25" id="KW-0456">Lyase</keyword>
    <keyword evidence="12 20" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="25" id="KW-0614">Plasmid</keyword>
    <keyword evidence="26" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="10 19" id="KW-0816">Tricarboxylic acid cycle</keyword>
    <feature type="domain" description="Aconitase_B_N" evidence="24">
        <location>
            <begin position="5"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="domain" description="Aconitase_2_N" evidence="23">
        <location>
            <begin position="168"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="domain" description="Aconitase" evidence="22">
        <location>
            <begin position="474"/>
            <end position="817"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="21">
        <location>
            <begin position="246"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="21">
        <location>
            <begin position="417"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <position position="191"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <position position="501"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="713"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="771"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="774"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <position position="793"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <position position="798"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004717"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00013250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00019379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PIRNR" id="PIRNR036687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PIRSR" id="PIRSR036687-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="PIRSR" id="PIRSR036687-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="Pfam" id="PF00330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="Pfam" id="PF06434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="Pfam" id="PF11791"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="EMBL" id="ABF12155.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="863" mass="93213" checksum="635FC873684365FC" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="96" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LCH8</accession>
    <name>Q1LCH8_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">3-oxoacyl-(Acyl-carrier-protein) reductase (3-ketoacyl-acyl carrier protein reductase)</fullName>
            <ecNumber evidence="1">1.1.1.100</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_5289</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EC" id="1.1.1.100" evidence="1"/>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12148.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519693.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5289"/>
    <dbReference type="EnsemblBacteria" id="ABF12148">
        <property type="protein sequence ID" value="ABF12148"/>
        <property type="gene ID" value="Rmet_5289"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5289"/>
    <dbReference type="eggNOG" id="COG4221">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010194_1_2_4"/>
    <dbReference type="OMA" id="SMKGAME"/>
    <dbReference type="OrthoDB" id="1209997at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004316">
        <property type="term" value="F:3-oxoacyl-[acyl-carrier-protein] reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF12148.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="252" mass="25598" checksum="869CEAB70E8A1BF2" modified="2006-05-30" version="1">MDLGLKDKRAIVTGGSRGIGKAIALQLAQEGVDVVVAARGRAALEATAAELATLTGRRIVPLAVDTSSKASVDSMVNQAIAALGGIDILVNAAALPGGISSASRLDEIDDAQAIEDIDIKVIGYLRTARALAPHLIANGWGRIVNIGGLAIYRTGRPIATLRNVGVAAITKNLADELGPKGINVTAVHPGATRTETTDAAFEAWAAARTSLGRIVEAREVAAVVAFLASPVSVAINGDAISVAGGSPGTIRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LCK9</accession>
    <name>Q1LCK9_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative flagellar protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_5258</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the type III secretion exporter family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12117.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519663.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5258"/>
    <dbReference type="EnsemblBacteria" id="ABF12117">
        <property type="protein sequence ID" value="ABF12117"/>
        <property type="gene ID" value="Rmet_5258"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5258"/>
    <dbReference type="eggNOG" id="COG2257">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041013_4_1_4"/>
    <dbReference type="OMA" id="HEVPIYE"/>
    <dbReference type="OrthoDB" id="1823885at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009306">
        <property type="term" value="P:protein secretion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1690.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006135">
        <property type="entry name" value="T3SS_substrate_exporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029025">
        <property type="entry name" value="T3SS_substrate_exporter_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30531">
        <property type="entry name" value="PTHR30531"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01312">
        <property type="entry name" value="Bac_export_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160544">
        <property type="entry name" value="SSF160544"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0966">Cell projection</keyword>
    <keyword evidence="2" id="KW-0969">Cilium</keyword>
    <keyword evidence="2" id="KW-0282">Flagellum</keyword>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF12117.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="91" mass="9711" checksum="AE3F9A67E5D8064D" modified="2006-05-30" version="1">MSDTSRGAAVALSYEEGEKAPRVVAKGYGVTAESIIARAQEAGVYVHNSPALVNLLMQVDMDSQIPPQLYLAVAQLLAWLHQIEDATVSQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-12-02" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LD40</accession>
    <name>Q1LD40_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">NADPH-dependent FMN reductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">msuE1</name>
        <name evidence="3" type="ordered locus">Rmet_5075</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SsuE family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11936.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5075"/>
    <dbReference type="EnsemblBacteria" id="ABF11936">
        <property type="protein sequence ID" value="ABF11936"/>
        <property type="gene ID" value="Rmet_5075"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5075"/>
    <dbReference type="eggNOG" id="COG0431">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_055322_3_3_4"/>
    <dbReference type="OMA" id="YAMRPLF"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029039">
        <property type="entry name" value="Flavoprotein-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005025">
        <property type="entry name" value="FMN_Rdtase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019912">
        <property type="entry name" value="FMN_Rdtase_MsuE-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03358">
        <property type="entry name" value="FMN_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52218">
        <property type="entry name" value="SSF52218"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03566">
        <property type="entry name" value="FMN_reduc_MsuE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FMN_red" evidence="2">
        <location>
            <begin position="10"/>
            <end position="154"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF03358"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF11936.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="197" mass="21677" checksum="865C0D769AF3429F" modified="2006-05-30" version="1">MNAKMTLPLRVVAVSGGLQRPSKSAALAEHLLDLIAEEVLCEPRLVEVGQLAPQLAGAVWRSQLPDTVERELAAVEQADVLVVATPVFRGSYTGLFKHFFDFIDQDALIDKPVLLAATGGSERHALMIDHQLRPLFSFFQARTLPLGVYATDKDFVDYRLQNGALIQRARLAVQRALPLVELTRHTRSANVEEVVAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="113" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LDE8</accession>
    <name>Q1LDE8_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Histidine kinase</fullName>
            <ecNumber evidence="2">2.7.13.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">Rmet_4966</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="11">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="11">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.</text>
            <dbReference type="EC" id="2.7.13.3"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.7.13.3" evidence="2"/>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11828.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519388.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4966"/>
    <dbReference type="EnsemblBacteria" id="ABF11828">
        <property type="protein sequence ID" value="ABF11828"/>
        <property type="gene ID" value="Rmet_4966"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4966"/>
    <dbReference type="eggNOG" id="COG0642">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_104_12_4"/>
    <dbReference type="OMA" id="INRIERC"/>
    <dbReference type="OrthoDB" id="1755994at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00082">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.160">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003661">
        <property type="entry name" value="HisK_dim/P"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036097">
        <property type="entry name" value="HisK_dim/P_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036641">
        <property type="entry name" value="HPT_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004358">
        <property type="entry name" value="Sig_transdc_His_kin-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001638">
        <property type="entry name" value="Solute-binding_3/MltF_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00512">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00497">
        <property type="entry name" value="SBP_bac_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00344">
        <property type="entry name" value="BCTRLSENSOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00388">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00062">
        <property type="entry name" value="PBPb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47226">
        <property type="entry name" value="SSF47226"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47384">
        <property type="entry name" value="SSF47384"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="SSF55874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="10" id="KW-0614">Plasmid</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7" id="KW-0732">Signal</keyword>
    <keyword evidence="10" id="KW-0808">Transferase</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193032" description="Histidine kinase" evidence="7">
        <location>
            <begin position="33"/>
            <end position="951"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="8">
        <location>
            <begin position="376"/>
            <end position="599"/>
        </location>
    </feature>
    <feature type="domain" description="Response regulatory" evidence="9">
        <location>
            <begin position="727"/>
            <end position="841"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="6">
        <location>
            <position position="776"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ABF11828.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="951" mass="102462" checksum="771B5B7DB437961A" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="100" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LDG4</accession>
    <name>Q1LDG4_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Putative transcriptional regulator, LacI family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Rmet_4950</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="6">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="6">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11812.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519371.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4950"/>
    <dbReference type="DNASU" id="4041812"/>
    <dbReference type="EnsemblBacteria" id="ABF11812">
        <property type="protein sequence ID" value="ABF11812"/>
        <property type="gene ID" value="Rmet_4950"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4950"/>
    <dbReference type="eggNOG" id="COG1609">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037628_6_1_4"/>
    <dbReference type="OMA" id="NPYFTEI"/>
    <dbReference type="OrthoDB" id="1796334at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01392">
        <property type="entry name" value="HTH_LacI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000843">
        <property type="entry name" value="HTH_LacI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046335">
        <property type="entry name" value="LacI/GalR-like_sensor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010982">
        <property type="entry name" value="Lambda_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028082">
        <property type="entry name" value="Peripla_BP_I"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00356">
        <property type="entry name" value="LacI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13377">
        <property type="entry name" value="Peripla_BP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00354">
        <property type="entry name" value="HTH_LACI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47413">
        <property type="entry name" value="SSF47413"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53822">
        <property type="entry name" value="SSF53822"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50932">
        <property type="entry name" value="HTH_LACI_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0614">Plasmid</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH lacI-type" evidence="4">
        <location>
            <begin position="6"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABF11812.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="341" mass="37130" checksum="BE92D6C05B28F0BF" modified="2006-05-30" version="1">MAEKQISIRDVAALAGVSVGSASRVINGAANVTPDVRERVDRAIAALRYRPNHAAQSLRSRTSKTIGCMFSDVANPLYARAFRALEEHFRREGYMLLLANGLNDAEREIDIIQTFQLRGMDGVILAPGNERHPGVLDAVRRLSMPAVIYDRDLAVDADAVLFDHVAGVKAAIHHLVELGHTRIALALWQVKSRPVRRRIEGYKAAFKAAGLPVPDLILQEPTPTSSAFDDVTRLLQLPDPPTAFLAQGTHTLTSTLRAIAKQGLRVPEDVSVIGIGDTDFAQTYDPPLTVLRTPTDEVAAHAVEMLLSRLAAKGDASLSPRKALIQFDLILRESCAPVARH</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="54" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LDH0</accession>
    <name>Q1LDH0_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">BON domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_4944</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11806.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008651028.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF11806">
        <property type="protein sequence ID" value="ABF11806"/>
        <property type="gene ID" value="Rmet_4944"/>
    </dbReference>
    <dbReference type="GeneID" id="60823806"/>
    <dbReference type="KEGG" id="rme:Rmet_4944"/>
    <dbReference type="HOGENOM" id="CLU_2397468_0_0_4"/>
    <dbReference type="OrthoDB" id="2214829at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007055">
        <property type="entry name" value="BON_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04972">
        <property type="entry name" value="BON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50914">
        <property type="entry name" value="BON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BON" evidence="1">
        <location>
            <begin position="17"/>
            <end position="86"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF11806.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="93" mass="9891" checksum="406B7597CEC26441" modified="2006-05-30" version="1">MMQTEIQASTWTSPDEEDELLRERIGTCIAEACGGDMPDGVAVHVVDRQVTITGRVEDSDLSRRIAQAAATPPGILGVTNLLVTRVPSQAQGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LDS4</accession>
    <name>Q1LDS4_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative ABC transporter, periplasmic binding component involved in Fe3+ transport</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_4840</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11702.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519266.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4840"/>
    <dbReference type="EnsemblBacteria" id="ABF11702">
        <property type="protein sequence ID" value="ABF11702"/>
        <property type="gene ID" value="Rmet_4840"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4840"/>
    <dbReference type="eggNOG" id="COG1840">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026974_3_1_4"/>
    <dbReference type="OMA" id="EWADWAS"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193097" evidence="1">
        <location>
            <begin position="30"/>
            <end position="340"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF11702.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="340" mass="37403" checksum="3EA6A364DFB3F9C9" modified="2006-05-30" version="1">MSTQSFWRRPLRWLAITATAAAFSQGVAAQTAICYNCPPEWADWAAQIRAIKEKTGITVPLDNKNSGQSLAQLVAERASPVADVTYLGVTFGIQAKADGVTQGYKPANWNDIPDGLKDPQGNWFAIHSGTLGFMVNVDALRGKPVPQSWADLLKPEYKGLIGYLDPASAFVGYVGAVAVNQAMGGTLDNFGPAFKYFRELQKNQPIVPKQTAYARVLSGEIPILLDYDFNAYRAKYKDHANVAFVIPKEGTLVVPYVMSLVNNAPHATEGRKVLDFVLSDQGQALWANAYLRPVRNVPMPKDVQSRFLPAAEYARAKPVDFGKMAQVQKAFSEQYLKEVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LDY4</accession>
    <name>Q1LDY4_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Putative branched-chain amino acid ABC transport system permease protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Rmet_4780</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="8">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="8">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11642.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519210.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4780"/>
    <dbReference type="EnsemblBacteria" id="ABF11642">
        <property type="protein sequence ID" value="ABF11642"/>
        <property type="gene ID" value="Rmet_4780"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4780"/>
    <dbReference type="eggNOG" id="COG4177">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031365_0_1_4"/>
    <dbReference type="OMA" id="YWMFWIG"/>
    <dbReference type="OrthoDB" id="1456257at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015658">
        <property type="term" value="F:branched-chain amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06581">
        <property type="entry name" value="TM_PBP1_LivM_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001851">
        <property type="entry name" value="ABC_transp_permease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043428">
        <property type="entry name" value="LivM-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30482">
        <property type="entry name" value="PTHR30482"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02653">
        <property type="entry name" value="BPD_transp_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0614">Plasmid</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="91"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="166"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="212"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="252"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="289"/>
            <end position="312"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABF11642.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="331" mass="35038" checksum="577408A272A2E359" modified="2006-05-30" version="1">MKTRVHRLPDGRWHPAEIVFWLAPVVAFFLLPDYLILGSQILIVGLFALSLDLILGYAGIVSLGHAAFFGLGAYMAGLLGAHGWGEPLTGLLAAAAVAALAGWLCSFLVIRGGDLTRLMVTLGIGLMLFEAANKAAFLTGGVDGLSGVTMNKILGMFEFDLFGKTAYVYSLVVLFVIFLLVRRLVASPFGLSLRGIREGARRMPALGTDVPARLRAVFTVAAAIAGVAGGLLAQTTQFVGMDVLGFPRSAELLVMLVLGGTGRLYGALVGAALFMIAQDVLAGINPVYWQFWIGLLLVVIVLFARGGVMGALSALGRRRHPSEPARKEVDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="102" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LE09</accession>
    <name>Q1LE09_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Glucans biosynthesis protein G</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">mdoG</name>
        <name evidence="6" type="synonym">opgG</name>
        <name evidence="10" type="ordered locus">Rmet_4755</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="11">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="11">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Involved in the biosynthesis of osmoregulated periplasmic glucans (OPGs).</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 6">Glycan metabolism; osmoregulated periplasmic glucan (OPG) biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Periplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 6">Belongs to the OpgD/OpgG family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11617.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4755"/>
    <dbReference type="EnsemblBacteria" id="ABF11617">
        <property type="protein sequence ID" value="ABF11617"/>
        <property type="gene ID" value="Rmet_4755"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4755"/>
    <dbReference type="eggNOG" id="COG3131">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023403_2_0_4"/>
    <dbReference type="OMA" id="KRPSAWI"/>
    <dbReference type="UniPathway" id="UPA00637"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042597">
        <property type="term" value="C:periplasmic space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009250">
        <property type="term" value="P:glucan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.98.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01069">
        <property type="entry name" value="MdoG_OpgG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011013">
        <property type="entry name" value="Gal_mutarotase_sf_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014718">
        <property type="entry name" value="GH-type_carb-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014438">
        <property type="entry name" value="Glucan_biosyn_MdoG/MdoD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007444">
        <property type="entry name" value="Glucan_biosyn_MdoG_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023704">
        <property type="entry name" value="MdoG_OpgG"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30504">
        <property type="entry name" value="PTHR30504"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04349">
        <property type="entry name" value="MdoG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006281">
        <property type="entry name" value="MdoG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF74650">
        <property type="entry name" value="SSF74650"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="SSF81296"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 6" id="KW-0574">Periplasm</keyword>
    <keyword evidence="10" id="KW-0614">Plasmid</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0732">Signal</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="89"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="domain" description="MdoG" evidence="9">
        <location>
            <begin position="108"/>
            <end position="583"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022764"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF04349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ABF11617.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="588" mass="66574" checksum="B459D7E06E3C661F" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LE29</accession>
    <name>Q1LE29_CUPMC</name>
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        <submittedName>
            <fullName evidence="7">Galactarate transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">garP</name>
        <name evidence="7" type="ordered locus">Rmet_4735</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="8">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="8">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11597.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008651304.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4735"/>
    <dbReference type="EnsemblBacteria" id="ABF11597">
        <property type="protein sequence ID" value="ABF11597"/>
        <property type="gene ID" value="Rmet_4735"/>
    </dbReference>
    <dbReference type="GeneID" id="60821676"/>
    <dbReference type="KEGG" id="rme:Rmet_4735"/>
    <dbReference type="eggNOG" id="COG2271">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001265_5_1_4"/>
    <dbReference type="OMA" id="CINVLTY"/>
    <dbReference type="OrthoDB" id="1345431at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000849">
        <property type="entry name" value="Sugar_P_transporter"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002808">
        <property type="entry name" value="Hexose_phosphate_transp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="SSF103473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0614">Plasmid</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="20"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="57"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="90"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="116"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="157"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="184"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="258"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="297"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="333"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="357"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="386"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="419"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="domain" description="MFS" evidence="6">
        <location>
            <begin position="24"/>
            <end position="445"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABF11597.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="453" mass="49096" checksum="AA4EAA38E211289D" modified="2006-05-30" version="1">MNATPTTGAAAASTARRTNVRYWILALIFIVTTVNYADRATLSITGPAMREEFGFSAVQMGYIFSAFSWSYVMAQIPGGWLLDRFGARRIYAVSIFLWSFFTLLQGWVGIFASMGATMTALFMLRFAVGLAESPAFPANAKVVASWFPTAERGTASAIFNAAQYFAAVVFTPLMAWIVHSMGWHHVYLWMGIMGMLLAMLWLKVMRSPVTHPAVNRAELEHIEQGGGLIHMNEGAGKSKQGANPAGWFYARQLLSNRMLAGVYLGQYCINVLTYFFLTWFPVYLVQARGMSILKAGFVASLPAICGFLGGVLGGVISDHLLRRGYSLTIARKVPIVAGMLLSVSMIACNYVDAEWLVVGIMALAFFGKGIGALGWAVVADTAPKEVIGLAGSIFNTFGNIAGIVTPIVIGYILSATGSFNGALVFVGINALVTVLSYLVIVKDIRRVELKHPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-12-02" version="92" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LE50</accession>
    <name>Q1LE50_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Two-component sensor histidine kinase</fullName>
            <ecNumber evidence="2">2.7.13.3</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_4714</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EC" id="2.7.13.3" evidence="2"/>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11576.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4714"/>
    <dbReference type="EnsemblBacteria" id="ABF11576">
        <property type="protein sequence ID" value="ABF11576"/>
        <property type="gene ID" value="Rmet_4714"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4714"/>
    <dbReference type="eggNOG" id="COG4585">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_735036_0_0_4"/>
    <dbReference type="OMA" id="IDTASHI"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00130">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000014">
        <property type="entry name" value="PAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035965">
        <property type="entry name" value="PAS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011712">
        <property type="entry name" value="Sig_transdc_His_kin_sub3_dim/P"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07730">
        <property type="entry name" value="HisKA_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13426">
        <property type="entry name" value="PAS_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00091">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55785">
        <property type="entry name" value="SSF55785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="SSF55874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00229">
        <property type="entry name" value="sensory_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50112">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0418">Kinase</keyword>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PAS" evidence="1">
        <location>
            <begin position="100"/>
            <end position="170"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF11576.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="447" mass="48875" checksum="CDE176CC0D92398D" modified="2006-05-30" version="1">MGRDPPSSSDGPLSWHCIFHAPRMITNDTRHASGVPARRSREHRPAWSGPPWRAFHRNVGIARICIGGPMEDKPSHGAEPLKLRDVTDAMRRMSAAIETGHRCFRAITDGRGTALAVLAPDGYFVSVNHGAARLLGYTCAELIGRSLADMADDGDRAWIVDCLAASISCTLRSFNVVLHGRDNRAASVLLHFQPVMDGRGNCSAVLVIFEEPVVLQQGLVDALMRSEADFRRLYTHLLTGQEHERKRLSSELHDGLGQALTLVKLMVEDALGRMRHGSAEQAESLLETTLLRIRETIGEVRHICNDLRPRLLDDLGLVPALEALCKQTQQCTGNLLVVFDCRIVENEVPDNLKADIFRIAQEAINNIIKHALATEINIVLRRAAKEILLTIQDNGIGFDDLSSSPDGPTFSGLGLLGMRERVESNGGSFALTSGQGSGTLVSAAWRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-10-07" version="54" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LE90</accession>
    <name>Q1LE90_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_4674</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11536.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4674"/>
    <dbReference type="EnsemblBacteria" id="ABF11536">
        <property type="protein sequence ID" value="ABF11536"/>
        <property type="gene ID" value="Rmet_4674"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4674"/>
    <dbReference type="HOGENOM" id="CLU_1609444_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="15"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="39"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF11536.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="165" mass="18428" checksum="9456D73C99BE51F4" modified="2006-05-30" version="1">MPTLVVVPCWRRARIWPAAAYFALASAASFRLYTLNPVAAPWAVALLALLLAIPTARLCVKKQYIFSCDPKSILREVRFLGLLLKRTSIDPMPFRWVRSRMSLLDPRDTIIELGKEHLYGALPVQTAKYAMGESSDVIATRATIARALGILDLGHERLPKQRAIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="61" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LEF4</accession>
    <name>Q1LEF4_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_4610</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11472.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519047.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4610"/>
    <dbReference type="EnsemblBacteria" id="ABF11472">
        <property type="protein sequence ID" value="ABF11472"/>
        <property type="gene ID" value="Rmet_4610"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4610"/>
    <dbReference type="eggNOG" id="COG3185">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012617_1_1_4"/>
    <dbReference type="OMA" id="DYLCFET"/>
    <dbReference type="OrthoDB" id="361195at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010839">
        <property type="entry name" value="AtuA"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07287">
        <property type="entry name" value="AtuA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF11472.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="451" mass="48661" checksum="9F2F1975A8D3576D" modified="2006-05-30" version="1">MTQKTTLRIGSGSGWWGDRVEPAERSARLGKLDYLCFETMAEATVSAAQVRKRRDPAFGGYDTYLDDRMRAVLPHCIANGTRIISNQGWIHPLGAARRVAELCEELGLSGVKIAAVTTTDLTGSICELDLTLFETGAPVSTLRETLISAEPYEGAGPIVEALRAGAQIVITGRVADPSLFLAPMIHHFGWQPDDVALLARGSAIGHLLECGAQVTGGYFGDPGYKDVPEPWNLAFPIAEVEADGSAVIAKVAGSGGRIDLQTVKEQMFYEVHDPRRYITPDVIVDFSTAVLEQVGPDRVRISGVSGLPRTDTLKVSLGCTEGHIGEDMFFYAGPGCLEKAKLAKRILEERFAMAALQADELRIDFLGVNAVHGVASPEPACEPNEIAVRVAARTRTKEEAAKVGREIDSMAVCGLASTGKRVPHQDRTREIIGVWSALVPRALIQSRVQFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LEH6</accession>
    <name>Q1LEH6_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Small multidrug resistance transmembrane protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">Rmet_4588</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="12">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="12">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11450.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008648542.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4588"/>
    <dbReference type="EnsemblBacteria" id="ABF11450">
        <property type="protein sequence ID" value="ABF11450"/>
        <property type="gene ID" value="Rmet_4588"/>
    </dbReference>
    <dbReference type="GeneID" id="60825942"/>
    <dbReference type="KEGG" id="rme:Rmet_4588"/>
    <dbReference type="eggNOG" id="COG2076">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_131462_2_1_4"/>
    <dbReference type="OMA" id="FGEMIGP"/>
    <dbReference type="OrthoDB" id="1944417at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009245">
        <property type="term" value="P:lipid A biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009103">
        <property type="term" value="P:lipopolysaccharide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042221">
        <property type="term" value="P:response to chemical"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000620">
        <property type="entry name" value="EamA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000390">
        <property type="entry name" value="Small_drug/metabolite_transptr"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30561">
        <property type="entry name" value="PTHR30561"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00892">
        <property type="entry name" value="EamA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5" id="KW-0441">Lipid A biosynthesis</keyword>
    <keyword evidence="3" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="7" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="6" id="KW-0448">Lipopolysaccharide biosynthesis</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-0614">Plasmid</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="48"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="78"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="104"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="domain" description="EamA" evidence="10">
        <location>
            <begin position="52"/>
            <end position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022556"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022985"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ABF11450.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="123" mass="13116" checksum="7CC4F76AFCD072D4" modified="2006-05-30" version="1">MTLSTFAFIFTGVLLNACAQLLLKAGVNAVGAITIDRATLFTTAFRVLTQWPVIGGLTLYVVSVAVWIVGLSRVDVSIAYPMLSLGYVVNALAAWWLFGEMIGPLRVAGILLILAGVFLIARS</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="56" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LFG4</accession>
    <name>Q1LFG4_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">LprI domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_4245</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11112.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518736.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4245"/>
    <dbReference type="EnsemblBacteria" id="ABF11112">
        <property type="protein sequence ID" value="ABF11112"/>
        <property type="gene ID" value="Rmet_4245"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4245"/>
    <dbReference type="eggNOG" id="ENOG5030YMT">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1748164_0_0_4"/>
    <dbReference type="OMA" id="ATIEMNL"/>
    <dbReference type="OrthoDB" id="1860689at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009739">
        <property type="entry name" value="LprI_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07007">
        <property type="entry name" value="LprI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193412" description="LprI domain-containing protein" evidence="1">
        <location>
            <begin position="26"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="domain" description="LprI" evidence="2">
        <location>
            <begin position="36"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF11112.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="144" mass="15532" checksum="3DE84A80A24F8C1E" modified="2006-05-30" version="1">MKTNVLHQVAAGFVFAAMCTQTALAGTTAETLYKQCAAKETNAEQRECYPAAVRQSEVELVAAEKKARAAMVDLERESEGSRSLQPVMAFDKAERAYRAFRTAESNRVVASYGSGNGGGLASYKATIEMNLTRIKQLQGESGGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LFU6</accession>
    <name>Q1LFU6_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Pyridoxal phosphate homeostasis protein</fullName>
            <shortName evidence="1">PLP homeostasis protein</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Rmet_4113</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="5">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="5">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Pyridoxal 5'-phosphate (PLP)-binding protein, which is involved in PLP homeostasis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the pyridoxal phosphate-binding protein YggS/PROSC family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10980.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518608.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4113"/>
    <dbReference type="EnsemblBacteria" id="ABF10980">
        <property type="protein sequence ID" value="ABF10980"/>
        <property type="gene ID" value="Rmet_4113"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4113"/>
    <dbReference type="eggNOG" id="COG0325">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_059988_1_0_4"/>
    <dbReference type="OMA" id="PLEWHMI"/>
    <dbReference type="OrthoDB" id="1423012at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02087">
        <property type="entry name" value="PLP_homeostasis"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001608">
        <property type="entry name" value="Ala_racemase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029066">
        <property type="entry name" value="PLP-binding_barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011078">
        <property type="entry name" value="PyrdxlP_homeostasis"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10146">
        <property type="entry name" value="PTHR10146"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01168">
        <property type="entry name" value="Ala_racemase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51419">
        <property type="entry name" value="SSF51419"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00044">
        <property type="entry name" value="TIGR00044"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0614">Plasmid</keyword>
    <keyword evidence="1" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ala_racemase_N" evidence="3">
        <location>
            <begin position="34"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="1">
        <location>
            <position position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF10980.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="286" mass="31441" checksum="93B3082C57C71EDA" modified="2006-05-30" version="1">MHTDPPDSVHPTQPLHDLHNRWPQAESVEDFRHNLAAVHARIAAACHRVGRDPAGVRLLPVSKAKPEASIRLAHAAGCRLLGENKPQEAYRKWEALADLEDLQWSVIGHLQTNKAKLAARFATEFQALDSLRVAEALDRRLQIEGRALDVFVQVNTSGEASKFGLHPDEVPGFLRALPAFSGLRVRGLMTLALLSAESERVRQCFVLLRELRERLRQDAPVGIGLDELSMGMSGDFEIAIEEGATVVRVGQAIFGARSTPDSFYWPEAEAGDSTLSAAHSGRQGER</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="98" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LG66</accession>
    <name>Q1LG66_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Peptidoglycan glycosyltransferase</fullName>
            <ecNumber evidence="4">2.4.1.129</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="primary">mrcB</name>
        <name evidence="19" type="ordered locus">Rmet_3992</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="20">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="20" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="20">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>[GlcNAc-(1-&gt;4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)](n)-di-trans,octa-cis-undecaprenyl diphosphate + beta-D-GlcNAc-(1-&gt;4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-di-trans,octa-cis-undecaprenyl diphosphate = [GlcNAc-(1-&gt;4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)](n+1)-di-trans-octa-cis-undecaprenyl diphosphate + di-trans,octa-cis-undecaprenyl diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:23708"/>
            <dbReference type="Rhea" id="RHEA-COMP:9602"/>
            <dbReference type="Rhea" id="RHEA-COMP:9603"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:58405"/>
            <dbReference type="ChEBI" id="CHEBI:60033"/>
            <dbReference type="ChEBI" id="CHEBI:78435"/>
            <dbReference type="EC" id="2.4.1.129"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cell wall biogenesis; peptidoglycan biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">In the C-terminal section; belongs to the transpeptidase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">In the N-terminal section; belongs to the glycosyltransferase 51 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.129" evidence="4"/>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10860.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3992"/>
    <dbReference type="CAZy" id="GT51">
        <property type="family name" value="Glycosyltransferase Family 51"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF10860">
        <property type="protein sequence ID" value="ABF10860"/>
        <property type="gene ID" value="Rmet_3992"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3992"/>
    <dbReference type="eggNOG" id="COG0744">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006354_2_4_4"/>
    <dbReference type="OMA" id="QQNTGGD"/>
    <dbReference type="UniPathway" id="UPA00219"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004180">
        <property type="term" value="F:carboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008658">
        <property type="term" value="F:penicillin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008955">
        <property type="term" value="F:peptidoglycan glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009252">
        <property type="term" value="P:peptidoglycan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008360">
        <property type="term" value="P:regulation of cell shape"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3810.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.710.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012338">
        <property type="entry name" value="Beta-lactam/transpept-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001264">
        <property type="entry name" value="Glyco_trans_51"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023346">
        <property type="entry name" value="Lysozyme-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036950">
        <property type="entry name" value="PBP_transglycosylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001460">
        <property type="entry name" value="PCN-bd_Tpept"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00912">
        <property type="entry name" value="Transgly"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00905">
        <property type="entry name" value="Transpeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53955">
        <property type="entry name" value="SSF53955"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56601">
        <property type="entry name" value="SSF56601"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0121">Carboxypeptidase</keyword>
    <keyword evidence="10" id="KW-0133">Cell shape</keyword>
    <keyword evidence="14" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="7 19" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="11" id="KW-0573">Peptidoglycan synthesis</keyword>
    <keyword evidence="19" id="KW-0614">Plasmid</keyword>
    <keyword evidence="6" id="KW-0645">Protease</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="19" id="KW-0808">Transferase</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Transgly" evidence="18">
        <location>
            <begin position="61"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="domain" description="Transpeptidase" evidence="17">
        <location>
            <begin position="331"/>
            <end position="567"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="636"/>
            <end position="687"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="16">
        <location>
            <begin position="645"/>
            <end position="664"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="672"/>
            <end position="687"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004752"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007739"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023988"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF00905"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF00912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="ABF10860.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="687" mass="73722" checksum="210BED86FAE15396" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LGC5</accession>
    <name>Q1LGC5_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Extra-cytoplasmic solute receptor protein involved in tricarboxylate transport</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">tctC</name>
        <name evidence="4" type="ordered locus">Rmet_3933</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="5">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="5">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Periplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the UPF0065 (bug) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10801.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518444.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3933"/>
    <dbReference type="EnsemblBacteria" id="ABF10801">
        <property type="protein sequence ID" value="ABF10801"/>
        <property type="gene ID" value="Rmet_3933"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3933"/>
    <dbReference type="eggNOG" id="COG3181">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045683_1_0_4"/>
    <dbReference type="OMA" id="DEAYAWW"/>
    <dbReference type="OrthoDB" id="988124at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042597">
        <property type="term" value="C:periplasmic space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005064">
        <property type="entry name" value="BUG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042100">
        <property type="entry name" value="Bug_dom1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42928">
        <property type="entry name" value="PTHR42928"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03401">
        <property type="entry name" value="TctC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017082">
        <property type="entry name" value="YflP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-0675">Receptor</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193433" evidence="3">
        <location>
            <begin position="21"/>
            <end position="311"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF10801.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="311" mass="33280" checksum="5648846DB20B1AF4" modified="2006-05-30" version="1">MRTLYLFALLLIGGVQTAHAAQAEMPQPRCIAPARPGGGYDLTCRIAKLGLDEARALPARLQIDYRPGGIGAVMFDAVVEQSPDDANTIVAFSSGALLNLASGKFSKYGPDSVKWLVAIGVDHGMIAVDARSPYLTLKDLVTAMKADPRKVLFGIGGSAGSQDWMKATLLARQAGLDRNAMRYVAFEGGGEAMTALMTGHVQAVPGDIGEAVDLIETHRIRVLAVLSDQRLPGRFASIPTAKEQGLDVQWPIVRGFYMGPKVAEHDLRRWGNALQRAAGTRAYVQALETHGIVPTPMLCISSFRDAESVGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="65" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LGE6</accession>
    <name>Q1LGE6_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative phosphoglycerate / bisphosphoglycerate mutase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_3912</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10780.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518427.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3912"/>
    <dbReference type="EnsemblBacteria" id="ABF10780">
        <property type="protein sequence ID" value="ABF10780"/>
        <property type="gene ID" value="Rmet_3912"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3912"/>
    <dbReference type="eggNOG" id="COG0406">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_084200_0_0_4"/>
    <dbReference type="OMA" id="WQIVNTS"/>
    <dbReference type="OrthoDB" id="1122642at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006094">
        <property type="term" value="P:gluconeogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd07067">
        <property type="entry name" value="HP_PGM_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1240">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013078">
        <property type="entry name" value="His_Pase_superF_clade-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029033">
        <property type="entry name" value="His_PPase_superfam"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00300">
        <property type="entry name" value="His_Phos_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00855">
        <property type="entry name" value="PGAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53254">
        <property type="entry name" value="SSF53254"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0312">Gluconeogenesis</keyword>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF10780.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="224" mass="24697" checksum="84F77116B679BF63" modified="2006-05-30" version="1">MATLFLVRHGQASFGAANYDCLSDTGRQQSRWLGEYFRDRGVQFRRVVAGTLVRQQDTASEILAGMGISDLPVASHPGLNEYDGESLYRSFTKGADHRAHQNGDYQDYWRTFRAAFAAWTQDQLTGMPETWGEFGQRMQAALAHATEGASREDALLVVSSGGAIGRAVADLLGAPTQTAIELNLQFRNTGFCELIVGRGTQRLLSFNNVPHLELPERRPAITFA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LGR2</accession>
    <name>Q1LGR2_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Putative transcriptional regulator, TetR family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_3794</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10664.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518312.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3794"/>
    <dbReference type="EnsemblBacteria" id="ABF10664">
        <property type="protein sequence ID" value="ABF10664"/>
        <property type="gene ID" value="Rmet_3794"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3794"/>
    <dbReference type="eggNOG" id="COG1309">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_099797_1_0_4"/>
    <dbReference type="OMA" id="VYERNTR"/>
    <dbReference type="OrthoDB" id="1437290at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036271">
        <property type="entry name" value="Tet_transcr_reg_TetR-rel_C_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48498">
        <property type="entry name" value="SSF48498"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF10664.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="200" mass="22309" checksum="EEFB3FAEC854354F" modified="2006-05-30" version="1">MATRRTDEAFLEELTHLLITTGVSSLTIGEMAQRMRCSRRRLYEIAETKEELFVHVCRNVLEANLEKGYAAARRAPDAARAISAYMHATLNATGLSKAALTDLDAIETGRKVFDDYQLARVRGLESMIEEGVRQGLMAPHNPRLVSEAILGAAHRLRNQQFLQQTGMKIGDAFSEFYELILNGLLVRPSAPTRRAKPPRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LGW2</accession>
    <name>Q1LGW2_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Peptidoglycan hydrolase FlgJ</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Muramidase FlgJ</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="primary">flgJ</name>
        <name evidence="10" type="ordered locus">Rmet_3742</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="11">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="11">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Flagellum-specific muramidase which hydrolyzes the peptidoglycan layer to assemble the rod structure in the periplasmic space.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Periplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">In the C-terminal section; belongs to the glycosyl hydrolase 73 family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">In the N-terminal section; belongs to the FlgJ family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10614.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518260.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3742"/>
    <dbReference type="CAZy" id="GH73">
        <property type="family name" value="Glycoside Hydrolase Family 73"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF10614">
        <property type="protein sequence ID" value="ABF10614"/>
        <property type="gene ID" value="Rmet_3742"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3742"/>
    <dbReference type="eggNOG" id="COG1705">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG3951">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013771_3_0_4"/>
    <dbReference type="OMA" id="EGVFVQM"/>
    <dbReference type="OrthoDB" id="682655at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042597">
        <property type="term" value="C:periplasmic space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004040">
        <property type="term" value="F:amidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016798">
        <property type="term" value="F:hydrolase activity, acting on glycosyl bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044780">
        <property type="term" value="P:bacterial-type flagellum assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008152">
        <property type="term" value="P:metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019301">
        <property type="entry name" value="Flagellar_prot_FlgJ_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013377">
        <property type="entry name" value="FlaJ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002901">
        <property type="entry name" value="MGlyc_endo_b_GlcNAc-like_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01832">
        <property type="entry name" value="Glucosaminidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10135">
        <property type="entry name" value="Rod-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00047">
        <property type="entry name" value="LYZ2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02541">
        <property type="entry name" value="flagell_FlgJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0574">Periplasm</keyword>
    <keyword evidence="10" id="KW-0614">Plasmid</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="LYZ2" evidence="9">
        <location>
            <begin position="182"/>
            <end position="339"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00013433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022764"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00030835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ABF10614.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="340" mass="36026" checksum="23B9566A96053695" modified="2006-05-30" version="1">MTGATLSGQSDISQRFALDTQGFEALKNQARAGNNSGSLQAAARQFEAVFTNMVLKSMRDATPSDGLFDNEQTKLYMSMMDQQLSQQLSTRGIGLADVMIRQLARATGQQLPPNMATAGGVTAASIQDAQMAGLLDGRGATDADGPALPVGTTLPGPAWNPTAGVRQYQNQADWQGNGQAQLGQLPNDTPEHISSFVNRMAGPAMVASRASGVPAKLIVGQAALESGWGRREILNSDGSTSFNVFGIKAGANWKGPTTEVLTTEYVDGQPQKVRARFRAYGSYEEACNDYARLLTSNPRYAGVVAANSADEAAHGLQRAGYATDPAYGEKLVRIMKKVST</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LGZ3</accession>
    <name>Q1LGZ3_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Acetyltranferase, GNAT family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_3711</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10583.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518231.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3711"/>
    <dbReference type="EnsemblBacteria" id="ABF10583">
        <property type="protein sequence ID" value="ABF10583"/>
        <property type="gene ID" value="Rmet_3711"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3711"/>
    <dbReference type="eggNOG" id="COG1670">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013985_3_1_4"/>
    <dbReference type="OMA" id="QVMRYFP"/>
    <dbReference type="OrthoDB" id="1497607at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008080">
        <property type="term" value="F:N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13302">
        <property type="entry name" value="Acetyltransf_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="SSF55729"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="1">
        <location>
            <begin position="9"/>
            <end position="178"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF10583.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="195" mass="21821" checksum="AEBB16730D63FCC4" modified="2006-05-30" version="1">MEPVPTARLRLRQWREEDRVPFRAINADEEIMAFFPSTLSDAQSDALMDRIRQHHAQHGFGLWALELRETSTFIGFTGLAIPSWAPPFGPCVEIGWRLAREHWGRGYATEAARATLDFGFGALRLAEIVSFTAAINQRSEQLMQRLGMHHAASDDFDHPALPSGDRLARHVLYRLSGAQWDGSTGTGSRIPDPGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LGZ4</accession>
    <name>Q1LGZ4_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Rubredoxin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Rmet_3710</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="9">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="9">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 6">
            <name>Fe(3+)</name>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the rubredoxin family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10582.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3710"/>
    <dbReference type="EnsemblBacteria" id="ABF10582">
        <property type="protein sequence ID" value="ABF10582"/>
        <property type="gene ID" value="Rmet_3710"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3710"/>
    <dbReference type="eggNOG" id="COG1773">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_128747_0_0_4"/>
    <dbReference type="OMA" id="QWVCVIC"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00730">
        <property type="entry name" value="rubredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024934">
        <property type="entry name" value="Rubredoxin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024935">
        <property type="entry name" value="Rubredoxin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018527">
        <property type="entry name" value="Rubredoxin_Fe_BS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00301">
        <property type="entry name" value="Rubredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00163">
        <property type="entry name" value="RUBREDOXIN"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00202">
        <property type="entry name" value="RUBREDOXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50903">
        <property type="entry name" value="RUBREDOXIN_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0249">Electron transport</keyword>
    <keyword evidence="5 6" id="KW-0408">Iron</keyword>
    <keyword evidence="3 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0614">Plasmid</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Rubredoxin-like" evidence="7">
        <location>
            <begin position="126"/>
            <end position="177"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001965"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003820"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50903"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ABF10582.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="179" mass="19693" checksum="CA250FCD7F846ED8" modified="2006-05-30" version="1">MECEVLTGRCRGLEFRRNAAMCEAVFDSHQPPLGFRMYKKGTSVEIQFSPQRLNDAAGDPYWVDLTIDEAQALLAQLQAHLAQAASGTTAPLVFSLEGPPVRADIAPARTKANAEEEPAANAESEFKQWVCIICGWVYDEAAGLPEDGIAPGTRWEDIPADWRCPECDVGKEDFAMVEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LH21</accession>
    <name>Q1LH21_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Methyl-accepting chemotaxis sensory transducer</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Rmet_3683</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="6">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="6">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10555.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518206.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3683"/>
    <dbReference type="EnsemblBacteria" id="ABF10555">
        <property type="protein sequence ID" value="ABF10555"/>
        <property type="gene ID" value="Rmet_3683"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3683"/>
    <dbReference type="eggNOG" id="COG0840">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_107_16_4"/>
    <dbReference type="OMA" id="DLSQMNW"/>
    <dbReference type="OrthoDB" id="429006at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004888">
        <property type="term" value="F:transmembrane signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006935">
        <property type="term" value="P:chemotaxis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004090">
        <property type="entry name" value="Chemotax_Me-accpt_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024478">
        <property type="entry name" value="HlyB_4HB_MCP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004089">
        <property type="entry name" value="MCPsignal_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12729">
        <property type="entry name" value="4HB_MCP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00015">
        <property type="entry name" value="MCPsignal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00260">
        <property type="entry name" value="CHEMTRNSDUCR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00283">
        <property type="entry name" value="MA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50111">
        <property type="entry name" value="CHEMOTAXIS_TRANSDUC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0614">Plasmid</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0807">Transducer</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="196"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="domain" description="Methyl-accepting transducer" evidence="4">
        <location>
            <begin position="278"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="538"/>
            <end position="560"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="581"/>
            <end position="613"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="593"/>
            <end position="613"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABF10555.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="613" mass="64628" checksum="454FDFAE97B927E0" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="115" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LHF0</accession>
    <name>Q1LHF0_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="13">FAD-dependent pyridine nucleotide-disulphide oxidoreductase</fullName>
            <ecNumber evidence="13">1.8.1.7</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ordered locus">Rmet_3554</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="7">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
        <text evidence="7">Binds 1 FAD per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 9">Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family.</text>
    </comment>
    <dbReference type="EC" id="1.8.1.7" evidence="13"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10426.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517970.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3554"/>
    <dbReference type="EnsemblBacteria" id="ABF10426">
        <property type="protein sequence ID" value="ABF10426"/>
        <property type="gene ID" value="Rmet_3554"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3554"/>
    <dbReference type="eggNOG" id="COG1249">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016755_2_2_4"/>
    <dbReference type="OMA" id="GGATQRM"/>
    <dbReference type="OrthoDB" id="267896at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004362">
        <property type="term" value="F:glutathione-disulfide reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045454">
        <property type="term" value="P:cell redox homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006749">
        <property type="term" value="P:glutathione metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.390.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023753">
        <property type="entry name" value="FAD/NAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016156">
        <property type="entry name" value="FAD/NAD-linked_Rdtase_dimer_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006322">
        <property type="entry name" value="Glutathione_Rdtase_euk/bac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001100">
        <property type="entry name" value="Pyr_nuc-diS_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004099">
        <property type="entry name" value="Pyr_nucl-diS_OxRdtase_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012999">
        <property type="entry name" value="Pyr_OxRdtase_I_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42737">
        <property type="entry name" value="PTHR42737"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07992">
        <property type="entry name" value="Pyr_redox_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02852">
        <property type="entry name" value="Pyr_redox_dim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000350">
        <property type="entry name" value="Mercury_reductase_MerA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="SSF51905"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55424">
        <property type="entry name" value="SSF55424"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00076">
        <property type="entry name" value="PYRIDINE_REDOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7 9" id="KW-0274">FAD</keyword>
    <keyword evidence="2 9" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="7" id="KW-0520">NAD</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4 9 13" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5 9" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pyr_redox_2" evidence="12">
        <location>
            <begin position="49"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="domain" description="Pyr_redox_dim" evidence="11">
        <location>
            <begin position="382"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="17"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="6">
        <location>
            <position position="484"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="94"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="212"/>
            <end position="219"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="305"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="346"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="disulfide bond" description="Redox-active" evidence="8">
        <location>
            <begin position="85"/>
            <end position="90"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR000350-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR000350-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR000350-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF02852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF07992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ABF10426.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="493" mass="52523" checksum="817D5A105BF3E6BE" modified="2006-05-30" version="1">MTKRQASTATTTKTTAKARRRASEVSAEPVVERVPRRKRSVRPTTREADFVVIGAGSGGVAAARRAASHGARVILVERDAIGGTCVNRGCVPKKMLSYGANWASILSTCLSHTGGKEDWRDASVRVNAEVARLNASYAQRLNEAGVEILHGDARLSGADEVVVGNEIIRTRKTLIATGAHPLALPVPGGELASSSDDVFTWQTVPASIAVIGGGYIAVEMASILSRYGVKVDLLVRGDRLLPKFDHDIAAALAEALAAKGVRLHFRADVTMLSQANGATEVCYTQQNNPGRTQTVRAQAVLAAIGRRPSIAELGLDALGVQLGERGGICVDRQFRTTVRSIHAIGDCMDRNLHLTPVAIAQGRWLADRLFGKRGDIADFDFVPTAVFSEPAIGAVGLTEAQAIEAAGGRADRVRTEIKRFVSLENRFGGVAQPSVFKLVFNARSGRVLGAHLMDNAAPEIIQTFAVALRLGVKDAHLKTTMPLHPTVAEELFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LHT8</accession>
    <name>Q1LHT8_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Transcriptional regulator, LysR-family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_3416</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the LysR transcriptional regulatory family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10288.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517852.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3416"/>
    <dbReference type="EnsemblBacteria" id="ABF10288">
        <property type="protein sequence ID" value="ABF10288"/>
        <property type="gene ID" value="Rmet_3416"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3416"/>
    <dbReference type="eggNOG" id="COG0583">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039613_6_5_4"/>
    <dbReference type="OMA" id="CRSATMP"/>
    <dbReference type="OrthoDB" id="990666at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005119">
        <property type="entry name" value="LysR_subst-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000847">
        <property type="entry name" value="Tscrpt_reg_HTH_LysR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00126">
        <property type="entry name" value="HTH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03466">
        <property type="entry name" value="LysR_substrate"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00039">
        <property type="entry name" value="HTHLYSR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50931">
        <property type="entry name" value="HTH_LYSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH lysR-type" evidence="2">
        <location>
            <begin position="3"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF10288.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="320" mass="34486" checksum="8B43190EA8A34DAD" modified="2006-05-30" version="1">MPVDLRAMQCFLAVAAAGSISRAAETLHIAQPALSLQIRHLEEALGVTLFMRSHKGVEPTVAGQRFEIHARDILKRLDIACEDVRDLMVDPEGSVAIGLPQSMAKILTVPIVREVIRRWPKVRLQVIELSTGYIPGHLLSGHIDIGLTFRAHANSGLQFDQIVDEDLVLVGPPGQFANGHTQAQPLADTVRLRDLNQYPMILPAGEHGLRALLDGYLRTQGVDLQILAEVNAIPELIALASAGVGCTVLSYASVCAEVRKGQLSAARICQPAVSRPVYLCRSATMPLSIAASAVLDLLTETVRTVLDDGSWPARIGEVAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="61" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LHU4</accession>
    <name>Q1LHU4_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Rmet_3410</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10282.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517846.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3410"/>
    <dbReference type="EnsemblBacteria" id="ABF10282">
        <property type="protein sequence ID" value="ABF10282"/>
        <property type="gene ID" value="Rmet_3410"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3410"/>
    <dbReference type="eggNOG" id="COG0392">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_052307_0_0_4"/>
    <dbReference type="OMA" id="WWVATVR"/>
    <dbReference type="OrthoDB" id="1585225at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022791">
        <property type="entry name" value="L-PG_synthase/AglD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03706">
        <property type="entry name" value="LPG_synthase_TM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="37"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="116"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="149"/>
            <end position="171"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABF10282.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="329" mass="35835" checksum="F3FF3C04CBE733B0" modified="2006-05-30" version="1">MKRFALLGALAGLLILTVVVATEGIAEVAHILEQAGWPLLWLVPIHLIALWFDAYGWRVLLAPADPEKRAGTGFLLWVATVREAVTRLLPTAGIGGELVGIRLAWRRVPDGTAVSASIVIEVMVTMFSHYLFALAGVLLLMSITQDSDHGLLVAIGLLLSLPIPALFAYALRHGAWFTRLESFAQRMLGDDHKLAALIDGARLDAQIRALCRHYGVLVRTLIWQMTGLVVGTLEVWWGLSLLGHPVGFGEALAIEALTLAARQVAFFIPAGLGVQEAVIVLLGTSFGIDPQTSLALALVKRAREVLFGVPALLSWQWVEWRQMRRRVAG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-05-20" modified="2022-08-03" version="106" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LI13</accession>
    <name>EFTU_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Elongation factor Tu</fullName>
            <shortName evidence="2">EF-Tu</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">tuf1</name>
        <name type="ordered locus">Rmet_3324</name>
    </gene>
    <gene>
        <name evidence="2" type="primary">tuf2</name>
        <name type="ordered locus">Rmet_3341</name>
    </gene>
    <organism>
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. EF-Tu/EF-1A subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10196.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10213.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517774.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q1LI13"/>
    <dbReference type="SMR" id="Q1LI13"/>
    <dbReference type="STRING" id="266264.Rmet_3324"/>
    <dbReference type="EnsemblBacteria" id="ABF10196">
        <property type="protein sequence ID" value="ABF10196"/>
        <property type="gene ID" value="Rmet_3324"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF10213">
        <property type="protein sequence ID" value="ABF10213"/>
        <property type="gene ID" value="Rmet_3341"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3324"/>
    <dbReference type="KEGG" id="rme:Rmet_3341"/>
    <dbReference type="eggNOG" id="COG0050">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007265_0_0_4"/>
    <dbReference type="OMA" id="EGDKEWG"/>
    <dbReference type="OrthoDB" id="621774at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01884">
        <property type="entry name" value="EF_Tu"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03697">
        <property type="entry name" value="EFTU_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00118_B">
        <property type="entry name" value="EF_Tu_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041709">
        <property type="entry name" value="EF-Tu_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004161">
        <property type="entry name" value="EFTu-like_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033720">
        <property type="entry name" value="EFTU_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031157">
        <property type="entry name" value="G_TR_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000795">
        <property type="entry name" value="T_Tr_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009001">
        <property type="entry name" value="Transl_elong_EF1A/Init_IF2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004541">
        <property type="entry name" value="Transl_elong_EFTu/EF1A_bac/org"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004160">
        <property type="entry name" value="Transl_elong_EFTu/EF1A_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00009">
        <property type="entry name" value="GTP_EFTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03144">
        <property type="entry name" value="GTP_EFTU_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03143">
        <property type="entry name" value="GTP_EFTU_D3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00315">
        <property type="entry name" value="ELONGATNFCT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="SSF50447"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50465">
        <property type="entry name" value="SSF50465"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00485">
        <property type="entry name" value="EF-Tu"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00301">
        <property type="entry name" value="G_TR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51722">
        <property type="entry name" value="G_TR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0251">Elongation factor</keyword>
    <keyword id="KW-0342">GTP-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000337485" description="Elongation factor Tu">
        <location>
            <begin position="1"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="domain" description="tr-type G">
        <location>
            <begin position="10"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="region of interest" description="G1" evidence="1">
        <location>
            <begin position="19"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="G2" evidence="1">
        <location>
            <begin position="60"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="region of interest" description="G3" evidence="1">
        <location>
            <begin position="81"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="region of interest" description="G4" evidence="1">
        <location>
            <begin position="136"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="region of interest" description="G5" evidence="1">
        <location>
            <begin position="174"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="19"/>
            <end position="26"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="81"/>
            <end position="85"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="136"/>
            <end position="139"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00118"/>
        </source>
    </evidence>
    <sequence length="396" mass="43088" checksum="9D327317CD26CFE3" modified="2006-05-30" version="1">MAKEKFERTKPHVNVGTIGHVDHGKTTLTAAIATVLAAKFGGAAKKYDEIDAAPEEKARGITINTAHVEYETANRHYAHVDCPGHADYVKNMITGAAQMDGAILVCSAADGPMPQTREHILLARQVGVPYIIVFLNKCDMVDDAELLELVEMEVRELLSKYEFPGDDTPIIKGSAKLALEGDKGELGEVAIMNLADALDTYIPTPERAVDGTFLMPVEDVFSISGRGTVVTGRIERGVIKVGEEIEIVGIRPTVKTTCTGVEMFRKLLDQGQAGDNVGLLLRGTKREDVERGQVLCKPGSIKPHTHFTGEVYILSKDEGGRHTPFFNNYRPQFYFRTTDVTGSIELPQDKEMVMPGDNVSITVKLIAPIAMEEGLRFAIREGGRTVGAGVVAKILD</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-12-12" modified="2022-08-03" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LI63</accession>
    <name>RPOA_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DNA-directed RNA polymerase subunit alpha</fullName>
            <shortName evidence="1">RNAP subunit alpha</shortName>
            <ecNumber evidence="1">2.7.7.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">RNA polymerase subunit alpha</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Transcriptase subunit alpha</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpoA</name>
        <name type="ordered locus">Rmet_3291</name>
    </gene>
    <organism>
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate + RNA(n+1)</text>
            <dbReference type="Rhea" id="RHEA:21248"/>
            <dbReference type="Rhea" id="RHEA-COMP:14527"/>
            <dbReference type="Rhea" id="RHEA-COMP:17342"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61557"/>
            <dbReference type="ChEBI" id="CHEBI:140395"/>
            <dbReference type="EC" id="2.7.7.6"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer. The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta' and 1 omega subunit. When a sigma factor is associated with the core the holoenzyme is formed, which can initiate transcription.</text>
    </comment>
    <comment type="domain">
        <text evidence="1">The N-terminal domain is essential for RNAP assembly and basal transcription, whereas the C-terminal domain is involved in interaction with transcriptional regulators and with upstream promoter elements.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the RNA polymerase alpha chain family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.6" evidence="1"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10163.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008642968.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q1LI63"/>
    <dbReference type="SMR" id="Q1LI63"/>
    <dbReference type="STRING" id="266264.Rmet_3291"/>
    <dbReference type="EnsemblBacteria" id="ABF10163">
        <property type="protein sequence ID" value="ABF10163"/>
        <property type="gene ID" value="Rmet_3291"/>
    </dbReference>
    <dbReference type="GeneID" id="60826626"/>
    <dbReference type="KEGG" id="rme:Rmet_3291"/>
    <dbReference type="eggNOG" id="COG0202">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_053084_0_0_4"/>
    <dbReference type="OMA" id="LMKFRNF"/>
    <dbReference type="OrthoDB" id="662686at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000428">
        <property type="term" value="C:DNA-directed RNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed 5'-3' RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.120.12">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1360.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00059">
        <property type="entry name" value="RNApol_bact_RpoA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011262">
        <property type="entry name" value="DNA-dir_RNA_pol_insert"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011263">
        <property type="entry name" value="DNA-dir_RNA_pol_RpoA/D/Rpb3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011773">
        <property type="entry name" value="DNA-dir_RpoA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036603">
        <property type="entry name" value="RBP11-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011260">
        <property type="entry name" value="RNAP_asu_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036643">
        <property type="entry name" value="RNApol_insert_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32108">
        <property type="entry name" value="PTHR32108"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01000">
        <property type="entry name" value="RNA_pol_A_bac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03118">
        <property type="entry name" value="RNA_pol_A_CTD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01193">
        <property type="entry name" value="RNA_pol_L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00662">
        <property type="entry name" value="RPOLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55257">
        <property type="entry name" value="SSF55257"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56553">
        <property type="entry name" value="SSF56553"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02027">
        <property type="entry name" value="rpoA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0804">Transcription</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000264529" description="DNA-directed RNA polymerase subunit alpha">
        <location>
            <begin position="1"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="region of interest" description="Alpha N-terminal domain (alpha-NTD)" evidence="1">
        <location>
            <begin position="1"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="region of interest" description="Alpha C-terminal domain (alpha-CTD)" evidence="1">
        <location>
            <begin position="247"/>
            <end position="326"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00059"/>
        </source>
    </evidence>
    <sequence length="326" mass="35601" checksum="F4E3AE371F12BFA4" modified="2006-05-30" version="1">MQTALLKPKIIAVEPLGDHHAKVVMEPFERGYGHTLGNALRRVLLSSMVGYAPTEVTIAGVVHEYSTIDGVQEDVVNLLLNLKGVVFKLHNRDEVTVSLRKDGEGVVTAADIELPHDVEIINPGHVIAHLSAGGKLDMQIKVEQGRGYVPGNVRKFGDEASKVIGRIVLDASFSPVRRVSYAVESARVEQRTDLDKLVMNIETDGVISPEEAIRQSARILVDQLSVFAALEGTESAAEAASSRAPQIDPILLRPVDDLELTVRSANCLKAENIYYIGDLIQRTENELLKTPNLGRKSLNEIKEVLASRGLTLGMKLENWPPAGLEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LI68</accession>
    <name>Q1LI68_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 11">Delta-aminolevulinic acid dehydratase</fullName>
            <ecNumber evidence="4 11">4.2.1.24</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">hemB</name>
        <name evidence="13" type="ordered locus">Rmet_3286</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 11">
            <text>2 5-aminolevulinate = H(+) + 2 H2O + porphobilinogen</text>
            <dbReference type="Rhea" id="RHEA:24064"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:58126"/>
            <dbReference type="ChEBI" id="CHEBI:356416"/>
            <dbReference type="EC" id="4.2.1.24"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Porphyrin-containing compound metabolism; protoporphyrin-IX biosynthesis; coproporphyrinogen-III from 5-aminolevulinate: step 1/4.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Homooctamer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 12">Belongs to the ALAD family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.24" evidence="4 11"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10158.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008642991.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3286"/>
    <dbReference type="EnsemblBacteria" id="ABF10158">
        <property type="protein sequence ID" value="ABF10158"/>
        <property type="gene ID" value="Rmet_3286"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3286"/>
    <dbReference type="eggNOG" id="COG0113">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035731_0_0_4"/>
    <dbReference type="OMA" id="YQMDYAN"/>
    <dbReference type="OrthoDB" id="677575at2"/>
    <dbReference type="UniPathway" id="UPA00251">
        <property type="reaction ID" value="UER00318"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004655">
        <property type="term" value="F:porphobilinogen synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006782">
        <property type="term" value="P:protoporphyrinogen IX biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001731">
        <property type="entry name" value="ALAD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030656">
        <property type="entry name" value="ALAD_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11458">
        <property type="entry name" value="PTHR11458"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00490">
        <property type="entry name" value="ALAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001415">
        <property type="entry name" value="Porphbilin_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00144">
        <property type="entry name" value="DALDHYDRTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM01004">
        <property type="entry name" value="ALAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00169">
        <property type="entry name" value="D_ALA_DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0350">Heme biosynthesis</keyword>
    <keyword evidence="7 11 13" id="KW-0456">Lyase</keyword>
    <keyword evidence="10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 11" id="KW-0627">Porphyrin biosynthesis</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Schiff-base intermediate with substrate" evidence="9">
        <location>
            <position position="199"/>
        </location>
    </feature>
    <feature type="active site" description="Schiff-base intermediate with substrate" evidence="9">
        <location>
            <position position="254"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="239"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001227"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00020771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR001415-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR001415-5"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU004161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ABF10158.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="330" mass="36652" checksum="8939B6E5E4C9A151" modified="2006-05-30" version="1">MSTFPEFRPRRMRRDDFSRRMMRENRLSPDNLIYPVFILEGQNQRQTVASMPGVERVSVDLLMPVAEDCVKLGIPVIALFPVIDPSLKTPDGIEATNPEGLVPRAVRALKDRFPELGVLTDVALDPYTSHGQDGVLDENGYVINDITVEILVKQALVQAEAGVDIVAPSDMMDGRIGAVRNALEEAGHIHTRIMAYSAKYASAFYGPFRDAVGSASNLGKGNKMTYQMDPANTDEALREVAQDIMEGADMVMVKPGMPYLDIVRRVKDEFRFPTYVYQVSGEYAMLKAAAQNGWLDHDKVMMESLLAFRRAGADGILTYFARDAARLLQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="105" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LI74</accession>
    <name>Q1LI74_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">RNA polymerase sigma L factor (ECF:FecI-like)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">rpoL</name>
        <name evidence="4" type="ordered locus">Rmet_3280</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the sigma-70 factor family. ECF subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10152.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517754.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3280"/>
    <dbReference type="EnsemblBacteria" id="ABF10152">
        <property type="protein sequence ID" value="ABF10152"/>
        <property type="gene ID" value="Rmet_3280"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3280"/>
    <dbReference type="eggNOG" id="COG1595">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047691_1_4_4"/>
    <dbReference type="OMA" id="PHLTRYA"/>
    <dbReference type="OrthoDB" id="1914729at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016987">
        <property type="term" value="F:sigma factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006352">
        <property type="term" value="P:DNA-templated transcription, initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050896">
        <property type="term" value="P:response to stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039425">
        <property type="entry name" value="RNA_pol_sigma-70-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014284">
        <property type="entry name" value="RNA_pol_sigma-70_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007627">
        <property type="entry name" value="RNA_pol_sigma70_r2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013249">
        <property type="entry name" value="RNA_pol_sigma70_r4_t2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013325">
        <property type="entry name" value="RNA_pol_sigma_r2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013324">
        <property type="entry name" value="RNA_pol_sigma_r3/r4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43133">
        <property type="entry name" value="PTHR43133"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04542">
        <property type="entry name" value="Sigma70_r2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08281">
        <property type="entry name" value="Sigma70_r4_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88659">
        <property type="entry name" value="SSF88659"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88946">
        <property type="entry name" value="SSF88946"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02937">
        <property type="entry name" value="sigma70-ECF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Sigma70_r2" evidence="2">
        <location>
            <begin position="12"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="domain" description="Sigma70_r4_2" evidence="3">
        <location>
            <begin position="103"/>
            <end position="156"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF04542"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF08281"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF10152.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="177" mass="19911" checksum="1F6B1702847EABF7" modified="2006-05-30" version="1">MEPFERQLVVLAPRLRRHARGLTGDAMLADDLVQDTLERALRYRWRFRLRPGALWGDGADGLLPWLLTLMHRLRLNHLRKSDVVTAMDTLPEVVAHTADLGLRRDLLQALGQLPEPQRAVLLLVSLEQFSYAEAARVLDVPIGTVMSRLARAREHMRRLLDGGADGAAATTSLQRVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LI82</accession>
    <name>Q1LI82_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Type IV pilus assembly protein PilM</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">pilM</name>
        <name evidence="2" type="ordered locus">Rmet_3272</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10144.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517744.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3272"/>
    <dbReference type="EnsemblBacteria" id="ABF10144">
        <property type="protein sequence ID" value="ABF10144"/>
        <property type="gene ID" value="Rmet_3272"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3272"/>
    <dbReference type="eggNOG" id="COG4972">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_050686_1_0_4"/>
    <dbReference type="OMA" id="EKVQDRQ"/>
    <dbReference type="OrthoDB" id="1750212at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005883">
        <property type="entry name" value="PilM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003494">
        <property type="entry name" value="SHS2_FtsA"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11104">
        <property type="entry name" value="PilM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF019169">
        <property type="entry name" value="PilM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00842">
        <property type="entry name" value="FtsA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="SSF53067"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01175">
        <property type="entry name" value="pilM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FtsA" evidence="1">
        <location>
            <begin position="14"/>
            <end position="181"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF10144.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="361" mass="38775" checksum="9E73FBAF5D846BC1" modified="2006-05-30" version="1">MRRGILSGLLRRTTVGVDIGSSSVKVVELSVGAGRNDFRLEKCASELLDRNAVADGNVVNIEAVGIALKRALGKAGIRTKEVVLGVPSMLTESQTVSLPDNLSEDELYSQVESEAHRLYPPSQAVNFDFAVIGPSETEGGIGVRVTAANSDRVQERVTAAEMAGLKPQVMDVEEYAVQRSIVQMLGVPEGLSEADAKDLPVVAVVHLGGSRSKAIFYQGWKELYEQPLNSYGDQLTQSAARMFTLDALKAEIKKRKNTLPESWRAQLLKPSLEALAMEVQGAIGNFIASSSLGRVDEILLSGGHASLLGVQAAIQQQTQITTTLANPFANMMTAGKVNERYLQRDLPAYIVSAGLALRGLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="114" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LIM2</accession>
    <name>Q1LIM2_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 7">UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-alanine ligase</fullName>
            <ecNumber evidence="6 7">6.3.2.10</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">D-alanyl-D-alanine-adding enzyme</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6 11" type="primary">murF</name>
        <name evidence="11" type="ordered locus">Rmet_3132</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6 7">Involved in cell wall formation. Catalyzes the final step in the synthesis of UDP-N-acetylmuramoyl-pentapeptide, the precursor of murein.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6 7">
            <text>ATP + D-alanyl-D-alanine + UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-gamma-D-glutamyl-meso-2,6-diaminoheptanedioate = ADP + H(+) + phosphate + UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-gamma-D-glutamyl-meso-2,6-diaminopimeloyl-D-alanyl-D-alanine</text>
            <dbReference type="Rhea" id="RHEA:28374"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57822"/>
            <dbReference type="ChEBI" id="CHEBI:61386"/>
            <dbReference type="ChEBI" id="CHEBI:83905"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.2.10"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="6 7">Cell wall biogenesis; peptidoglycan biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6 7">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the MurCDEF family. MurF subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.3.2.10" evidence="6 7"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10004.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517622.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3132"/>
    <dbReference type="EnsemblBacteria" id="ABF10004">
        <property type="protein sequence ID" value="ABF10004"/>
        <property type="gene ID" value="Rmet_3132"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3132"/>
    <dbReference type="eggNOG" id="COG0770">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031507_4_0_4"/>
    <dbReference type="OMA" id="VEMGANH"/>
    <dbReference type="OrthoDB" id="1861122at2"/>
    <dbReference type="UniPathway" id="UPA00219"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047480">
        <property type="term" value="F:UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008766">
        <property type="term" value="F:UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate-D-alanyl-D-alanine ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007049">
        <property type="term" value="P:cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009252">
        <property type="term" value="P:peptidoglycan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008360">
        <property type="term" value="P:regulation of cell shape"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1190.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02019">
        <property type="entry name" value="MurF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036565">
        <property type="entry name" value="Mur-like_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004101">
        <property type="entry name" value="Mur_ligase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036615">
        <property type="entry name" value="Mur_ligase_C_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013221">
        <property type="entry name" value="Mur_ligase_cen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000713">
        <property type="entry name" value="Mur_ligase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035911">
        <property type="entry name" value="MurE/MurF_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005863">
        <property type="entry name" value="UDP-N-AcMur_synth"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01225">
        <property type="entry name" value="Mur_ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02875">
        <property type="entry name" value="Mur_ligase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08245">
        <property type="entry name" value="Mur_ligase_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53244">
        <property type="entry name" value="SSF53244"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53623">
        <property type="entry name" value="SSF53623"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63418">
        <property type="entry name" value="SSF63418"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01143">
        <property type="entry name" value="murF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 6 7" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="1 6 7" id="KW-0132">Cell division</keyword>
    <keyword evidence="2 6 7" id="KW-0133">Cell shape</keyword>
    <keyword evidence="5 6 7" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 11" id="KW-0436">Ligase</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3 6 7" id="KW-0573">Peptidoglycan synthesis</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Mur_ligase" evidence="8">
        <location>
            <begin position="30"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="domain" description="Mur_ligase_M" evidence="10">
        <location>
            <begin position="110"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="domain" description="Mur_ligase_C" evidence="9">
        <location>
            <begin position="323"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="112"/>
            <end position="118"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU004136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF02875"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF08245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ABF10004.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="466" mass="48628" checksum="4DA6440FC648C7A4" modified="2006-05-30" version="1">MNRQPMTTLQQAAGWIAGASISGDATCSFSRVHTDSRTVEPGDLFVALKGERFDAHDFLADVVARGAAAVMVSRKVDVDVPALIVPDTRTGLGQLATGWRRTFTPPTVAVTGSNGKTTVKEMIAAIFAAAVGEGDRLATGGNLNNDIGLPLTVLRLRAHHKLAVLELGMNHPGETIYLAGIAQPTVAVVTNAQREHQEFMVSVEAVAHEHASAIAALPADGVAVFPVDAESGGAYAAIWRQAAGTRKVLSFGTDASADVRATVSLENGVQVLDVTAPDRRFTLRLHVLGVHNVRNALAATACALAAGVDVAAIQAGIAGFTAVKGRLQVKHTPAGTVVIDDTYNANPDSMRAAIDVLAGFATPRLLVLGDMGEVGDQGPMFHTEIGAYARERGIDALWATGELAPHAVQAYGGGARYFARAEDLAQALEADKEGTVANAAAVLVKGSRFMRMERMVDALVASPSAH</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-10-31" modified="2022-08-03" version="104" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LIM4</accession>
    <name>MURD_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">UDP-N-acetylmuramoylalanine--D-glutamate ligase</fullName>
            <ecNumber evidence="1">6.3.2.9</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">D-glutamic acid-adding enzyme</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">murD</name>
        <name type="ordered locus">Rmet_3130</name>
    </gene>
    <organism>
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Cell wall formation. Catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + D-glutamate + UDP-N-acetyl-alpha-D-muramoyl-L-alanine = ADP + H(+) + phosphate + UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamate</text>
            <dbReference type="Rhea" id="RHEA:16429"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29986"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:83898"/>
            <dbReference type="ChEBI" id="CHEBI:83900"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.2.9"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cell wall biogenesis; peptidoglycan biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the MurCDEF family.</text>
    </comment>
    <dbReference type="EC" id="6.3.2.9" evidence="1"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10002.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517621.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q1LIM4"/>
    <dbReference type="SMR" id="Q1LIM4"/>
    <dbReference type="STRING" id="266264.Rmet_3130"/>
    <dbReference type="EnsemblBacteria" id="ABF10002">
        <property type="protein sequence ID" value="ABF10002"/>
        <property type="gene ID" value="Rmet_3130"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3130"/>
    <dbReference type="eggNOG" id="COG0771">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032540_1_1_4"/>
    <dbReference type="OMA" id="CNFENYL"/>
    <dbReference type="OrthoDB" id="1093223at2"/>
    <dbReference type="UniPathway" id="UPA00219"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004326">
        <property type="term" value="F:tetrahydrofolylpolyglutamate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008764">
        <property type="term" value="F:UDP-N-acetylmuramoylalanine-D-glutamate ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007049">
        <property type="term" value="P:cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009252">
        <property type="term" value="P:peptidoglycan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008360">
        <property type="term" value="P:regulation of cell shape"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1190.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00639">
        <property type="entry name" value="MurD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018109">
        <property type="entry name" value="Folylpolyglutamate_synth_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036565">
        <property type="entry name" value="Mur-like_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004101">
        <property type="entry name" value="Mur_ligase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036615">
        <property type="entry name" value="Mur_ligase_C_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013221">
        <property type="entry name" value="Mur_ligase_cen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005762">
        <property type="entry name" value="MurD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02875">
        <property type="entry name" value="Mur_ligase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08245">
        <property type="entry name" value="Mur_ligase_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53244">
        <property type="entry name" value="SSF53244"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53623">
        <property type="entry name" value="SSF53623"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01087">
        <property type="entry name" value="murD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0131">Cell cycle</keyword>
    <keyword id="KW-0132">Cell division</keyword>
    <keyword id="KW-0133">Cell shape</keyword>
    <keyword id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0573">Peptidoglycan synthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000257222" description="UDP-N-acetylmuramoylalanine--D-glutamate ligase">
        <location>
            <begin position="1"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="129"/>
            <end position="135"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00639"/>
        </source>
    </evidence>
    <sequence length="504" mass="53563" checksum="714A1E689FD7530F" modified="2006-05-30" version="1">MFGVLQKPHVLVLGLGESGLAMARWCGLNGCRVRVADTREAPANLVFLQAELTTAQFMGGQFTENLLDDIGLVAISPGLSPLEPNTRALLEAAQARSIPVWGEIELFAQAIGYLEATSGYAPRVLAITGTNGKTTTTALTGRLIERAGKTVGVAGNISPSALDKLSACIASATLPDVWVLELSSFQLEYTFSLAPHAATVLNVTQDHLDWHGSMEAYAAAKARIFGPAEKGCLQVLNRQDPLTMNMARRGTTLVTFGTDLPETPGSYGVLREGGMPWLVLAEPDTEADAEQKPRRRKKDDVAADAVVPVRHKRLMPADALHIRGMHNATNAMAALALCRAIDLPLNALLHGLREYRGEPHRVEWVATIDEVEYFDDSKGTNVGATVAALSGLDKHVVLIAGGEGKGQDFSPLVAPVAQYARAVVLIGKDAGALREALGATGKPLIDAGSLEEAVEKSASLAEAGDVVLLSPACASLDMFRNYVHRAQVFRGAVEELALSRGIMP</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-10-07" version="57" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LIX3</accession>
    <name>Q1LIX3_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_3030</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09903.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3030"/>
    <dbReference type="EnsemblBacteria" id="ABF09903">
        <property type="protein sequence ID" value="ABF09903"/>
        <property type="gene ID" value="Rmet_3030"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3030"/>
    <dbReference type="eggNOG" id="ENOG5033E15">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2056245_0_0_4"/>
    <dbReference type="OMA" id="EHAGACC"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="26"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="93"/>
            <end position="130"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF09903.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="130" mass="14083" checksum="D6D1FDA759F62C88" modified="2006-05-30" version="1">MPTSLSRPFGFNPLTPSRLRRCALRLPPGDRRKAHPRRASLAPLDAAEPSMPDRPGQRPAAMDGAGEHAGACCGRLCECVPSSTLAVLAHHVTKDGSRTSRPASLWSFHATPQDRRRKVRPGRSRLSSDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LIY8</accession>
    <name>Q1LIY8_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative Signal transduction histidine kinase</fullName>
            <ecNumber evidence="3">2.7.13.3</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">hmzS</name>
        <name evidence="3" type="ordered locus">Rmet_3015</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.7.13.3" evidence="3"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09888.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517533.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3015"/>
    <dbReference type="EnsemblBacteria" id="ABF09888">
        <property type="protein sequence ID" value="ABF09888"/>
        <property type="gene ID" value="Rmet_3015"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3015"/>
    <dbReference type="eggNOG" id="COG5002">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_89_37_4"/>
    <dbReference type="OMA" id="FTLWFID"/>
    <dbReference type="OrthoDB" id="692375at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00082">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003661">
        <property type="entry name" value="HisK_dim/P"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036097">
        <property type="entry name" value="HisK_dim/P_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00512">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00388">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47384">
        <property type="entry name" value="SSF47384"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="SSF55874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="24"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="172"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="2">
        <location>
            <begin position="256"/>
            <end position="460"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF09888.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="470" mass="52098" checksum="F78DD85824DB0A4C" modified="2006-05-30" version="1">MVRPDSEALSDASQGGSWSLQRRLAASLVICIGGTFAILFPVLDYRIDHEIYRRMDTTLMQRSAAVGRVLQELDARKLESLMPEYEHGGHTEFFTIFDSQTHRTLLRSPSSAGAELSLGPVEQGTPRYYDVTLPDGHAGRALATRVSVTGERSQLLVVATERRDWDQTERRIHFALLGGIVLATLLATGLALLMVRHIVVMLERVGEQLADLRSDRPIARIGADFPRELRPFAEAFNQGLHHLYAAIVRERRFSRDVAHELRTPLAEIRISAENALIDADPTRIQRALNAMIQASSRMQRSVDTLLLLARLESGQHTLALDPLDLTALLQELLAGLNVQQMAPKSPIRVTLPESAWVQSDQGVIERIVSNLLRNAIEYAPECDDIQCRLEREASGWMLTIVNTAPNLQASDLEQFGLRFWRKDSEGGTAHHAGLGLALALALAHAIDLPLAFSLDNGRLSARLGPWPPLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="107" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LJ65</accession>
    <name>Q1LJ65_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 3">Pyrroline-5-carboxylate reductase</fullName>
            <shortName evidence="2">P5C reductase</shortName>
            <shortName evidence="2">P5CR</shortName>
            <ecNumber evidence="2 3">1.5.1.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">PCA reductase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="2 6" type="primary">proC</name>
        <name evidence="6" type="ordered locus">Rmet_2938</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Catalyzes the reduction of 1-pyrroline-5-carboxylate (PCA) to L-proline.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>L-proline + NAD(+) = 1-pyrroline-5-carboxylate + 2 H(+) + NADH</text>
            <dbReference type="Rhea" id="RHEA:14105"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15893"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:60039"/>
            <dbReference type="EC" id="1.5.1.2"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2 3">
            <text>L-proline + NADP(+) = 1-pyrroline-5-carboxylate + 2 H(+) + NADPH</text>
            <dbReference type="Rhea" id="RHEA:14109"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15893"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:60039"/>
            <dbReference type="EC" id="1.5.1.2"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 3">Amino-acid biosynthesis; L-proline biosynthesis; L-proline from L-glutamate 5-semialdehyde: step 1/1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 2 3">Belongs to the pyrroline-5-carboxylate reductase family.</text>
    </comment>
    <dbReference type="EC" id="1.5.1.2" evidence="2 3"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09811.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517479.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2938"/>
    <dbReference type="EnsemblBacteria" id="ABF09811">
        <property type="protein sequence ID" value="ABF09811"/>
        <property type="gene ID" value="Rmet_2938"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2938"/>
    <dbReference type="eggNOG" id="COG0345">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_042344_0_1_4"/>
    <dbReference type="OMA" id="VVRVMTN"/>
    <dbReference type="OrthoDB" id="1349288at2"/>
    <dbReference type="UniPathway" id="UPA00098">
        <property type="reaction ID" value="UER00361"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004735">
        <property type="term" value="F:pyrroline-5-carboxylate reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055129">
        <property type="term" value="P:L-proline biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01925">
        <property type="entry name" value="P5C_reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008927">
        <property type="entry name" value="6-PGluconate_DH-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028939">
        <property type="entry name" value="P5C_Rdtase_cat_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029036">
        <property type="entry name" value="P5CR_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000304">
        <property type="entry name" value="Pyrroline-COOH_reductase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11645">
        <property type="entry name" value="PTHR11645"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03807">
        <property type="entry name" value="F420_oxidored"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14748">
        <property type="entry name" value="P5CR_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000193">
        <property type="entry name" value="Pyrrol-5-carb_rd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48179">
        <property type="entry name" value="SSF48179"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00112">
        <property type="entry name" value="proC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00521">
        <property type="entry name" value="P5CR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 3" id="KW-0521">NADP</keyword>
    <keyword evidence="2 3 6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="2 3" id="KW-0641">Proline biosynthesis</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F420_oxidored" evidence="4">
        <location>
            <begin position="7"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="domain" description="P5CR_dimer" evidence="5">
        <location>
            <begin position="163"/>
            <end position="266"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01925"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003903"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF03807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF14748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABF09811.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="273" mass="28148" checksum="76F5289C9A3CDAAB" modified="2006-05-30" version="1">MLDSLTFGFLGGGNMASALIGGLVARGVPANAIRVVDPFEDARQRLATTLGVQTLAAPDAAFGASNVIVLAVKPQQFKDAVGALAPQLKNSLIISVAAGIRLQDMQRWLGTNRLVRAMPNTPALAGMGMTGLAAAPGLSDEDRAIAKAVAEAVGQCVWVEGDDQIDAVTAISGSGPAYVFYFVEAMERAAIEMGLSAEQGRKLAVETFRGAAALAAESSEPVSTLRERVTSKGGTTYAALTSMDASGIQDAFVRAMLAAAARGREMGAEFGKD</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2007-08-21" modified="2022-05-25" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LJA2</accession>
    <name>PPNP_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Pyrimidine/purine nucleoside phosphorylase</fullName>
            <ecNumber evidence="1">2.4.2.1</ecNumber>
            <ecNumber evidence="1">2.4.2.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Adenosine phosphorylase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Cytidine phosphorylase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Guanosine phosphorylase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Inosine phosphorylase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Thymidine phosphorylase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Uridine phosphorylase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Xanthosine phosphorylase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">ppnP</name>
        <name type="ordered locus">Rmet_2901</name>
    </gene>
    <organism>
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the phosphorolysis of diverse nucleosides, yielding D-ribose 1-phosphate and the respective free bases. Can use uridine, adenosine, guanosine, cytidine, thymidine, inosine and xanthosine as substrates. Also catalyzes the reverse reactions.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a purine D-ribonucleoside + phosphate = a purine nucleobase + alpha-D-ribose 1-phosphate</text>
            <dbReference type="Rhea" id="RHEA:19805"/>
            <dbReference type="ChEBI" id="CHEBI:26386"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57720"/>
            <dbReference type="ChEBI" id="CHEBI:142355"/>
            <dbReference type="EC" id="2.4.2.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>adenosine + phosphate = adenine + alpha-D-ribose 1-phosphate</text>
            <dbReference type="Rhea" id="RHEA:27642"/>
            <dbReference type="ChEBI" id="CHEBI:16335"/>
            <dbReference type="ChEBI" id="CHEBI:16708"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57720"/>
            <dbReference type="EC" id="2.4.2.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>cytidine + phosphate = alpha-D-ribose 1-phosphate + cytosine</text>
            <dbReference type="Rhea" id="RHEA:52540"/>
            <dbReference type="ChEBI" id="CHEBI:16040"/>
            <dbReference type="ChEBI" id="CHEBI:17562"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57720"/>
            <dbReference type="EC" id="2.4.2.2"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>guanosine + phosphate = alpha-D-ribose 1-phosphate + guanine</text>
            <dbReference type="Rhea" id="RHEA:13233"/>
            <dbReference type="ChEBI" id="CHEBI:16235"/>
            <dbReference type="ChEBI" id="CHEBI:16750"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57720"/>
            <dbReference type="EC" id="2.4.2.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>inosine + phosphate = alpha-D-ribose 1-phosphate + hypoxanthine</text>
            <dbReference type="Rhea" id="RHEA:27646"/>
            <dbReference type="ChEBI" id="CHEBI:17368"/>
            <dbReference type="ChEBI" id="CHEBI:17596"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57720"/>
            <dbReference type="EC" id="2.4.2.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>phosphate + thymidine = 2-deoxy-alpha-D-ribose 1-phosphate + thymine</text>
            <dbReference type="Rhea" id="RHEA:16037"/>
            <dbReference type="ChEBI" id="CHEBI:17748"/>
            <dbReference type="ChEBI" id="CHEBI:17821"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57259"/>
            <dbReference type="EC" id="2.4.2.2"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>phosphate + uridine = alpha-D-ribose 1-phosphate + uracil</text>
            <dbReference type="Rhea" id="RHEA:24388"/>
            <dbReference type="ChEBI" id="CHEBI:16704"/>
            <dbReference type="ChEBI" id="CHEBI:17568"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57720"/>
            <dbReference type="EC" id="2.4.2.2"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>phosphate + xanthosine = alpha-D-ribose 1-phosphate + xanthine</text>
            <dbReference type="Rhea" id="RHEA:27638"/>
            <dbReference type="ChEBI" id="CHEBI:17712"/>
            <dbReference type="ChEBI" id="CHEBI:18107"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57720"/>
            <dbReference type="EC" id="2.4.2.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the nucleoside phosphorylase PpnP family.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.1" evidence="1"/>
    <dbReference type="EC" id="2.4.2.2" evidence="1"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09774.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008644958.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q1LJA2"/>
    <dbReference type="SMR" id="Q1LJA2"/>
    <dbReference type="STRING" id="266264.Rmet_2901"/>
    <dbReference type="EnsemblBacteria" id="ABF09774">
        <property type="protein sequence ID" value="ABF09774"/>
        <property type="gene ID" value="Rmet_2901"/>
    </dbReference>
    <dbReference type="GeneID" id="60820663"/>
    <dbReference type="KEGG" id="rme:Rmet_2901"/>
    <dbReference type="eggNOG" id="COG3123">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_157874_1_0_4"/>
    <dbReference type="OMA" id="YHYICHF"/>
    <dbReference type="OrthoDB" id="1937865at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047975">
        <property type="term" value="F:guanosine phosphorylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004731">
        <property type="term" value="F:purine-nucleoside phosphorylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016154">
        <property type="term" value="F:pyrimidine-nucleoside phosphorylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009032">
        <property type="term" value="F:thymidine phosphorylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004850">
        <property type="term" value="F:uridine phosphorylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01537">
        <property type="entry name" value="Nucleos_phosphorylase_PpnP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009664">
        <property type="entry name" value="Ppnp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011051">
        <property type="entry name" value="RmlC_Cupin_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36540">
        <property type="entry name" value="PTHR36540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06865">
        <property type="entry name" value="Ppnp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51182">
        <property type="entry name" value="SSF51182"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0328">Glycosyltransferase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000298719" description="Pyrimidine/purine nucleoside phosphorylase">
        <location>
            <begin position="1"/>
            <end position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01537"/>
        </source>
    </evidence>
    <sequence length="103" mass="11205" checksum="0C99B6FA773F6A42" modified="2006-05-30" version="1">MSQFDNVSVVKKANLYFDGKCVSHTVTFADGTRKTLGVIFPAALTFNTGAPEIMEINAGVCRVRLAGSDEWKTYGAGQQFDVPGNSSFDIEVTETLDYVCHFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LJJ2</accession>
    <name>Q1LJJ2_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Uroporphyrinogen-III C-methyltransferase</fullName>
            <ecNumber evidence="2">2.1.1.107</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">cysG</name>
        <name evidence="10" type="ordered locus">Rmet_2811</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="7">Porphyrin-containing compound metabolism; siroheme biosynthesis; precorrin-2 from uroporphyrinogen III: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the precorrin methyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.107" evidence="2"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09684.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517380.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2811"/>
    <dbReference type="EnsemblBacteria" id="ABF09684">
        <property type="protein sequence ID" value="ABF09684"/>
        <property type="gene ID" value="Rmet_2811"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2811"/>
    <dbReference type="eggNOG" id="COG0007">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
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        <property type="reaction ID" value="UER00211"/>
    </dbReference>
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        <property type="component" value="Chromosome"/>
    </dbReference>
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        <property type="project" value="UniProtKB-EC"/>
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        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="P:siroheme biosynthetic process"/>
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        <property type="project" value="UniProtKB-UniPathway"/>
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        <property type="entry name" value="SUMT"/>
        <property type="match status" value="1"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="4pyrrol_Mease"/>
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        <property type="entry name" value="4pyrrol_Methylase_sf"/>
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    <dbReference type="InterPro" id="IPR014777">
        <property type="entry name" value="4pyrrole_Mease_sub1"/>
    </dbReference>
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        <property type="entry name" value="CobA/CysG_C"/>
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        <property type="entry name" value="Uropor_MeTrfase_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00590">
        <property type="entry name" value="TP_methylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53790">
        <property type="entry name" value="SSF53790"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="cobA_cysG_Cterm"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SUMT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SUMT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 8 10" id="KW-0489">Methyltransferase</keyword>
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    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="4 8 10" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="TP_methylase" evidence="9">
        <location>
            <begin position="11"/>
            <end position="217"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023244"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00025705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00590"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ABF09684.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="254" mass="26982" checksum="877EF82F69832E53" modified="2006-05-30" version="1">MDRQHGKATGKVYLIGAGPGAADLITVRGARLLGEAQVVLHDALVSPEMLAWCPQAELVEVGKRCGQRSTAQLFINRQIVDLATKHERVVRLKGGDPMLFGRADEELQALEAAGIEYEVVPGITAALAAASAIGRPLTKRGVSRSVAFATQAKAADAVDVEADVKADTIIYYMGRDQAANIATQLIARGKPASTPAWVVEAATTPRQRSHQFTLGQMAAGEAARWMDPVQPSLLMIGEALGSRATEYVEAERAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="103" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LJK5</accession>
    <name>Q1LJK5_CUPMC</name>
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        <submittedName>
            <fullName evidence="6">Transcriptional regulator, regulatory protein GntR-family, HTH:GntR, C-terminal</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">matR</name>
        <name evidence="6" type="ordered locus">Rmet_2798</name>
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        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
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        <lineage>
            <taxon>Bacteria</taxon>
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            <taxon>Betaproteobacteria</taxon>
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            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="ABF09671.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2798"/>
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        <property type="protein sequence ID" value="ABF09671"/>
        <property type="gene ID" value="Rmet_2798"/>
    </dbReference>
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        <property type="taxonomic scope" value="Bacteria"/>
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    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07377">
        <property type="entry name" value="WHTH_GntR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.530">
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="GntR_C"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Tscrpt_reg_HTH_GntR"/>
    </dbReference>
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        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH gntR-type" evidence="5">
        <location>
            <begin position="6"/>
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        </location>
    </feature>
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        <location>
            <begin position="211"/>
            <end position="233"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABF09671.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="233" mass="25641" checksum="8C5B960FE0BF053B" modified="2006-05-30" version="1">MRIVQQALYLEVADRLRAMIDDHTLLPGAWIDEVALAAALGTSRTPMREALKVLAAEGLVRLEPRRGCFVNALTEQDLDEIFPLMALLEGRCAYEAARNVTQTDLDALEGLHAELARHAAAGDIDAYYETNYQIHEAIQRLAGNRWLSGVVSDLRKVLRLSRHKSLKLPGRIQESCAEHMSVFSALKAHDPEGAEALMKTHVLRQRGALSALDRADPEPPAVAPGQLRLAGQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LJN2</accession>
    <name>Q1LJN2_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Rmet_2771</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09644.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517341.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2771"/>
    <dbReference type="EnsemblBacteria" id="ABF09644">
        <property type="protein sequence ID" value="ABF09644"/>
        <property type="gene ID" value="Rmet_2771"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2771"/>
    <dbReference type="eggNOG" id="COG2911">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002338_3_0_4"/>
    <dbReference type="OMA" id="NTAKQWP"/>
    <dbReference type="OrthoDB" id="754526at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005887">
        <property type="term" value="C:integral component of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009306">
        <property type="term" value="P:protein secretion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007452">
        <property type="entry name" value="TamB"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04357">
        <property type="entry name" value="TamB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="29"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="329"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1373"/>
            <end position="1392"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABF09644.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="1392" mass="145868" checksum="8C08D064D9381358" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-10-07" version="57" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LJX1</accession>
    <name>Q1LJX1_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_2682</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09555.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2682"/>
    <dbReference type="EnsemblBacteria" id="ABF09555">
        <property type="protein sequence ID" value="ABF09555"/>
        <property type="gene ID" value="Rmet_2682"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2682"/>
    <dbReference type="HOGENOM" id="CLU_1947044_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="43"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF09555.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="129" mass="13630" checksum="0943C2D8E8284597" modified="2006-05-30" version="1">MVARLHPCSPSLTSCRGHVLFEALLSLAFVGLGAIPLGMLGTVSLRWTGEQERLTTALMRAAELAEASAMALQPPGSDALPIPLCRLAEQKERCAPDKRLAIATIESRSPTDAAGVATMPTRLALWVAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LJY5</accession>
    <name>Q1LJY5_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">AAA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_2668</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09541.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517242.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2668"/>
    <dbReference type="REBASE" id="17790">
        <property type="entry name" value="RmeAMcrBCP"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF09541">
        <property type="protein sequence ID" value="ABF09541"/>
        <property type="gene ID" value="Rmet_2668"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2668"/>
    <dbReference type="eggNOG" id="COG1401">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_863093_0_0_4"/>
    <dbReference type="OMA" id="YQYEDFM"/>
    <dbReference type="OrthoDB" id="489714at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011704">
        <property type="entry name" value="ATPase_dyneun-rel_AAA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07728">
        <property type="entry name" value="AAA_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA" evidence="1">
        <location>
            <begin position="57"/>
            <end position="217"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF09541.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="303" mass="34372" checksum="02C78897E0364FC1" modified="2006-05-30" version="1">MLEKLEKVRSLSAAEMGFASPANSEAEADALNSSVTGPELDESDEKYLLTEALLADGFAGVVFSGPPGTGKSRYAREIARLFVEDDEARVFYVQFHPGYQYEDFMESFVPTSSGGFAPKDKVFLQACRRASEVDGPVVLVIDELSRTDVVRVFGEALTYLEKSKRGLSFQLASGRTTSIPNNLIMLATMNPWDRGVEEMDLALERRFARISIEPDPQALEIILSRSALTEERRLRVMRFFQTLYRHVNPLCRIGHAYFLTAQNDDSLKRLWDNQLSFHFERILKRSPEELERIRNGWQAIMAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="65" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LK09</accession>
    <name>Q1LK09_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Putative outer membrane biogenesis protein (YhjG-like)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Rmet_2640</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09517.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517216.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2640"/>
    <dbReference type="EnsemblBacteria" id="ABF09517">
        <property type="protein sequence ID" value="ABF09517"/>
        <property type="gene ID" value="Rmet_2640"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2640"/>
    <dbReference type="eggNOG" id="COG2982">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017234_1_1_4"/>
    <dbReference type="OMA" id="AWVPWPH"/>
    <dbReference type="OrthoDB" id="1068066at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007844">
        <property type="entry name" value="AsmA"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05170">
        <property type="entry name" value="AsmA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="domain" description="AsmA" evidence="3">
        <location>
            <begin position="6"/>
            <end position="587"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="200"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="693"/>
            <end position="735"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="707"/>
            <end position="735"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF05170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF09517.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="735" mass="78570" checksum="5EE4D7426C9D7ADC" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="97" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LK63</accession>
    <name>Q1LK63_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Transcriptional regulator, IclR-family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Rmet_2586</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09463.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2586"/>
    <dbReference type="EnsemblBacteria" id="ABF09463">
        <property type="protein sequence ID" value="ABF09463"/>
        <property type="gene ID" value="Rmet_2586"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2586"/>
    <dbReference type="eggNOG" id="COG1414">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_062618_0_1_4"/>
    <dbReference type="OMA" id="PRMLRPI"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029016">
        <property type="entry name" value="GAF-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014757">
        <property type="entry name" value="Tscrpt_reg_IclR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005471">
        <property type="entry name" value="Tscrpt_reg_IclR_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09339">
        <property type="entry name" value="HTH_IclR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01614">
        <property type="entry name" value="IclR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00346">
        <property type="entry name" value="HTH_ICLR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51077">
        <property type="entry name" value="HTH_ICLR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51078">
        <property type="entry name" value="ICLR_ED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH iclR-type" evidence="3">
        <location>
            <begin position="79"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="domain" description="IclR-ED" evidence="4">
        <location>
            <begin position="140"/>
            <end position="324"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABF09463.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="325" mass="35711" checksum="EAE2C0543769A4D0" modified="2006-05-30" version="1">MITFIAVSWFFWFFLAFPDCPAIVVFAEPRFANICSENAYHTDRQPGRKPGLTLGYLSLISTPMPDDHAPEIPKKEELLDSLTKGLALLRLYASGVERLTMQEVADELDVTRAAARRLLLTLAHNGYLAQEGRQFALTPKVMDLGYAYFASMNLPQLARPYLQALCAEVGESCSVGMLDQESVVLVAREEPSQLLRVDMAIGRRMPAYAHSLGRVLLAGLDAEALDAYLAQAELRQLTPFTISSRPALARKLEAVRADGFCTLISELADGFAGISVPLHDQTGKTAAGLGLSMVLGSRDQAYLEKHYLPPLRRTAAQIESILKAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LKG3</accession>
    <name>Q1LKG3_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Succinate dehydrogenase cytochrome b556 subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">sdhC</name>
        <name evidence="11" type="ordered locus">Rmet_2486</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
        <text evidence="9">The heme is bound between the two transmembrane subunits.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Part of an enzyme complex containing four subunits: a flavoprotein, an iron-sulfur protein, plus two membrane-anchoring proteins, SdhC and SdhD. The complex can form homotrimers.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09363.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008649352.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2486"/>
    <dbReference type="EnsemblBacteria" id="ABF09363">
        <property type="protein sequence ID" value="ABF09363"/>
        <property type="gene ID" value="Rmet_2486"/>
    </dbReference>
    <dbReference type="GeneID" id="60821039"/>
    <dbReference type="KEGG" id="rme:Rmet_2486"/>
    <dbReference type="eggNOG" id="COG2009">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_094691_2_0_4"/>
    <dbReference type="OMA" id="SWAFLHH"/>
    <dbReference type="OrthoDB" id="2046680at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045281">
        <property type="term" value="C:succinate dehydrogenase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000104">
        <property type="term" value="F:succinate dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1300.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034804">
        <property type="entry name" value="SQR/QFR_C/D"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014314">
        <property type="entry name" value="Succ_DH_cytb556"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000701">
        <property type="entry name" value="SuccDH_FuR_B_TM-su"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01127">
        <property type="entry name" value="Sdh_cyt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000178">
        <property type="entry name" value="SDH_cyt_b560"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81343">
        <property type="entry name" value="SSF81343"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02970">
        <property type="entry name" value="succ_dehyd_cytB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 9" id="KW-0349">Heme</keyword>
    <keyword evidence="6 9" id="KW-0408">Iron</keyword>
    <keyword evidence="7 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="33"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="74"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="110"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="9">
        <location>
            <position position="89"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
            <note>ligand shared with second transmembrane subunit</note>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00020076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00025912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR000178-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ABF09363.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="136" mass="15080" checksum="04E091118CB5BC78" modified="2006-05-30" version="1">MAEAVKQARPEFRNIGIAQISRYRLPWAGKVSILHRVSGAMMFLLLPFVLYLFEQSVTSELSFAKFSALLSNGFIKVVILALIWGYLHHFCAGIRFLLLDVHVGVTKPASAKSAVTVLVVSLLLTLIFGLKLFGLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LKP9</accession>
    <name>Q1LKP9_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">traR2</name>
        <name evidence="3" type="ordered locus">Rmet_2400</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the LysR transcriptional regulatory family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09277.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004350675.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2400"/>
    <dbReference type="EnsemblBacteria" id="ABF09277">
        <property type="protein sequence ID" value="ABF09277"/>
        <property type="gene ID" value="Rmet_2400"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2400"/>
    <dbReference type="eggNOG" id="COG0583">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039613_6_4_4"/>
    <dbReference type="OMA" id="SHDLTMK"/>
    <dbReference type="OrthoDB" id="1439189at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005119">
        <property type="entry name" value="LysR_subst-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000847">
        <property type="entry name" value="Tscrpt_reg_HTH_LysR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00126">
        <property type="entry name" value="HTH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03466">
        <property type="entry name" value="LysR_substrate"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00039">
        <property type="entry name" value="HTHLYSR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50931">
        <property type="entry name" value="HTH_LYSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH lysR-type" evidence="2">
        <location>
            <begin position="1"/>
            <end position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF09277.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="296" mass="32805" checksum="91CBD16BB01ED75C" modified="2006-05-30" version="1">MELRHLRYFMALAEELHFTRAAERLHIEQPPLSRAIKELEDELGVVLFDRNRRGTVLTSAGVVFLQDIRRLFTVLEQARENAQAVAAGLRGSLRIAISDGAIDARLSAFLARCRAEEPEIEIRLSEVPLAEQLRGLRSGDFMIGFAHTADVGDDIRAEPIWRDPLVIAVPARHALLAHKKVPLQEVGIHPLVLCDPHACEGYCRELTRLLQVLEHKLNVVEEVSSLDMMLTLVGAGYGIGFLAASKIPVCQRPDVVIRPLAIDSAVITTYLLRADSSDLSASLERFIVRLRDSASG</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="62" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LKU7</accession>
    <name>Q1LKU7_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Putative secreted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_2352</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09229.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_003149753.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2352"/>
    <dbReference type="EnsemblBacteria" id="ABF09229">
        <property type="protein sequence ID" value="ABF09229"/>
        <property type="gene ID" value="Rmet_2352"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2352"/>
    <dbReference type="eggNOG" id="ENOG502ZBPP">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_125516_0_0_4"/>
    <dbReference type="OMA" id="QFKRLPN"/>
    <dbReference type="OrthoDB" id="1854465at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022262">
        <property type="entry name" value="Lipoprot_put"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03751">
        <property type="entry name" value="conj_TIGR03751"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF09229.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="146" mass="16169" checksum="38365A6EB6A31727" modified="2006-05-30" version="1">MLSNSIKGLVLALAVAVLGGCATSKEKLLTHGDRTMMDIWQQEAGDGGGAAGRNAGRQLLDARQSLRRPLTDADVQAAPVEQMRYTRTARNEVHRQFQRLPNPDLVMYVYPHLAGTDPVPVPGYTTVFPLYQRIQYAMPGERVEDY</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-04-07" version="50" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LKZ2</accession>
    <name>Q1LKZ2_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_2305</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09184.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_003108988.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2305"/>
    <dbReference type="EnsemblBacteria" id="ABF09184">
        <property type="protein sequence ID" value="ABF09184"/>
        <property type="gene ID" value="Rmet_2305"/>
    </dbReference>
    <dbReference type="GeneID" id="56409813"/>
    <dbReference type="KEGG" id="rme:Rmet_2305"/>
    <dbReference type="eggNOG" id="ENOG502Z8IB">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2261334_0_0_4"/>
    <dbReference type="OrthoDB" id="1705324at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF09184.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="103" mass="11615" checksum="6F584611EEDD1B86" modified="2006-05-30" version="1">MLMSPVRLAWQVQVVRRGFLAVSARFAGPFAPNKRNHACVTTKVRPRQLLFLKGIKLNAIADRPAYFASLRARRGQPIVILAELGPDEAFALWKQHVLGDRPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="92" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LL13</accession>
    <name>Q1LL13_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative DNA-binding transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">yeiE</name>
        <name evidence="3" type="ordered locus">Rmet_2284</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the LysR transcriptional regulatory family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09163.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516988.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2284"/>
    <dbReference type="EnsemblBacteria" id="ABF09163">
        <property type="protein sequence ID" value="ABF09163"/>
        <property type="gene ID" value="Rmet_2284"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2284"/>
    <dbReference type="eggNOG" id="COG0583">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039613_6_1_4"/>
    <dbReference type="OMA" id="IEGPCHH"/>
    <dbReference type="OrthoDB" id="750230at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005119">
        <property type="entry name" value="LysR_subst-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000847">
        <property type="entry name" value="Tscrpt_reg_HTH_LysR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00126">
        <property type="entry name" value="HTH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03466">
        <property type="entry name" value="LysR_substrate"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50931">
        <property type="entry name" value="HTH_LYSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH lysR-type" evidence="2">
        <location>
            <begin position="8"/>
            <end position="65"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF09163.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="305" mass="33189" checksum="D5BB4E59E0596CF7" modified="2006-05-30" version="1">MDQRPLRLTLRQLQVFVAVAQHGSTIAAADALAMSQSAVSASLAELERALNGALFDRVARRLAINEAGRQFFPRALSLLDQAQELERFASEGGVQLRIAASNTIGSYILPALLAGFRNAQTGPCALDLRIGNTQDVLQSLMKFEADIGLIEGTSQERDLRSVHWCDDEMVVVVGPTHPLAVAGEQSPQFHDMLRDADWIVREPGSGTREIIEARLVPELGALRFALELGNAEAIKRAVMSGFGVSCLSLHVVRDEIERGLLVALRSDLPRVIRPLQLVVHQDKFPTQGLLAFTEYLRGVAPSLGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="98" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LL61</accession>
    <name>Q1LL61_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Formyltetrahydrofolate deformylase</fullName>
            <ecNumber evidence="3">3.5.1.10</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">Formyl-FH(4) hydrolase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="3 5" type="primary">purU</name>
        <name evidence="5" type="ordered locus">Rmet_2236</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Catalyzes the hydrolysis of 10-formyltetrahydrofolate (formyl-FH4) to formate and tetrahydrofolate (FH4).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>(6S)-10-formyltetrahydrofolate + H2O = (6S)-5,6,7,8-tetrahydrofolate + formate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19833"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15740"/>
            <dbReference type="ChEBI" id="CHEBI:57453"/>
            <dbReference type="ChEBI" id="CHEBI:57454"/>
            <dbReference type="EC" id="3.5.1.10"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Purine metabolism; IMP biosynthesis via de novo pathway; formate from 10-formyl-5,6,7,8-tetrahydrofolate: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the PurU family.</text>
    </comment>
    <dbReference type="EC" id="3.5.1.10" evidence="3"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09115.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008641549.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2236"/>
    <dbReference type="EnsemblBacteria" id="ABF09115">
        <property type="protein sequence ID" value="ABF09115"/>
        <property type="gene ID" value="Rmet_2236"/>
    </dbReference>
    <dbReference type="GeneID" id="60821191"/>
    <dbReference type="KEGG" id="rme:Rmet_2236"/>
    <dbReference type="eggNOG" id="COG0788">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038395_3_0_4"/>
    <dbReference type="OMA" id="ISNHRDF"/>
    <dbReference type="OrthoDB" id="979667at2"/>
    <dbReference type="UniPathway" id="UPA00074">
        <property type="reaction ID" value="UER00170"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008864">
        <property type="term" value="F:formyltetrahydrofolate deformylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016742">
        <property type="term" value="F:hydroxymethyl-, formyl- and related transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006730">
        <property type="term" value="P:one-carbon metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04875">
        <property type="entry name" value="ACT_F4HF-DF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd08648">
        <property type="entry name" value="FMT_core_Formyl-FH4-Hydrolase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01927">
        <property type="entry name" value="PurU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045865">
        <property type="entry name" value="ACT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002912">
        <property type="entry name" value="ACT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041729">
        <property type="entry name" value="Formyl-FH4-Hydrolase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002376">
        <property type="entry name" value="Formyl_transf_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036477">
        <property type="entry name" value="Formyl_transf_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004810">
        <property type="entry name" value="PurU"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044074">
        <property type="entry name" value="PurU_ACT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42706">
        <property type="entry name" value="PTHR42706"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01842">
        <property type="entry name" value="ACT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00551">
        <property type="entry name" value="Formyl_trans_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01575">
        <property type="entry name" value="FFH4HYDRLASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53328">
        <property type="entry name" value="SSF53328"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55021">
        <property type="entry name" value="SSF55021"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00655">
        <property type="entry name" value="PurU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51671">
        <property type="entry name" value="ACT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3 5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1 3" id="KW-0554">One-carbon metabolism</keyword>
    <keyword evidence="3" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ACT" evidence="4">
        <location>
            <begin position="7"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="active site" evidence="3">
        <location>
            <position position="232"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABF09115.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="288" mass="31913" checksum="6B8D640391EF4E7A" modified="2006-05-30" version="1">MSSTGFILTLSCPDQPGIVHAVSGLLFQHGCNIVDSDQYGDAFAGRFFMRVHFSAAPGGPDLAELRAAFAPVGDQFGMQWEVHDATVKPRVMIMVSKIGHCLNDLLFRAKVGGLPVEIAAIVSNHRDFYQLAASYDVPFFHLPLMNASAEQKAAQEARVFEVVREQNIDLVVLARYMQVLSDDLCRKLQGRAINIHHSFLPSFKGAKPYYQAHDRGVKLIGATAHYVTADLDEGPIIEQEIERVDHSMDPEQLTAVGRDVECVALARAVKWHAEHRILLNGHKTVVFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="97" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LLF3</accession>
    <name>Q1LLF3_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Permease component of ABC superfamily transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">yejE</name>
        <name evidence="7" type="ordered locus">Rmet_2144</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cell membrane</location>
            <topology evidence="5">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the binding-protein-dependent transport system permease family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09023.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516851.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2144"/>
    <dbReference type="EnsemblBacteria" id="ABF09023">
        <property type="protein sequence ID" value="ABF09023"/>
        <property type="gene ID" value="Rmet_2144"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2144"/>
    <dbReference type="eggNOG" id="COG4239">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028518_1_0_4"/>
    <dbReference type="OMA" id="PSPPTWM"/>
    <dbReference type="OrthoDB" id="1734230at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025966">
        <property type="entry name" value="OppC_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12911">
        <property type="entry name" value="OppC_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="SSF161098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="32"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="157"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="202"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="319"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="6">
        <location>
            <begin position="153"/>
            <end position="341"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABF09023.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="376" mass="42159" checksum="D7B5682F06436E1C" modified="2006-05-30" version="1">MNPFKRAPIQNGLPHSLSPRQRAWQRFRRNRLGYWSLILFVALFVLSLCAEVLSSDRPLLVRYKGEYYLPIAKVYPETTFDGDFPTRTDYLDPFILERITTHGNFAIFPLNRYSYDSINYFAKEPNPAPPSAENWLGTDDRGRDVLARLLYGFRVSVLFGLALTVIGVLVGTLTGALMGFFGGRFDLMSQRAIEIWGSMPELYLLIIFASIFEPSLALLIILLSLFGWMGLSDYVRAEFYRNRSLDYVKSARALGLSNVQIMWRHILPNSLTPVITFLPFRMSAAILALTSLDFLGLGVPPTTPSLGELLAQGKANLDAWWISLSTFAVLVLTLLLLTFMGDALRDAFDTRMGLAQMRGRVAPKVPARGGAAEAVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LLI0</accession>
    <name>Q1LLI0_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Protein-L-isoaspartate O-methyltransferase</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">L-isoaspartyl protein carboxyl methyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="3">Protein L-isoaspartyl methyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="4">Protein-beta-aspartate methyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Rmet_2117</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the methyltransferase superfamily. L-isoaspartyl/D-aspartyl protein methyltransferase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08996.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516830.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2117"/>
    <dbReference type="EnsemblBacteria" id="ABF08996">
        <property type="protein sequence ID" value="ABF08996"/>
        <property type="gene ID" value="Rmet_2117"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2117"/>
    <dbReference type="eggNOG" id="COG2518">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_055432_1_0_4"/>
    <dbReference type="OMA" id="HYGDGML"/>
    <dbReference type="OrthoDB" id="1948290at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004719">
        <property type="term" value="F:protein-L-isoaspartate (D-aspartate) O-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000682">
        <property type="entry name" value="PCMT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11579">
        <property type="entry name" value="PTHR11579"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00080">
        <property type="entry name" value="pimt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01279">
        <property type="entry name" value="PCMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005369"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013346"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00030757"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00031323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00031350"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABF08996.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="302" mass="31613" checksum="E26D31536C69AE07" modified="2006-05-30" version="1">MSSTPRRSRFPLPLDAVVERKPAPARTAGMPAVGKRAASPAPASTAVKPRKPAAPPVAPPPPGSAVEARASAAVAGGGGMASDRARAALAARLRAGGIRDERVLGAIATVPRHLFMEPGLASQAYEDAALPIGHQQTISKPSVVARMIELLREGMPSDHALARVLEIGTGCGYQAAVLSLVAQEVFSIERIRPLHEQAKANLRPLRVPNIRLHYGDGMLGLPQAAPFSSIILAAAGMEVPQALLEQLAIGGRLIAPVVAPSSGAPGQTVTQQLLLIERLNRHRFHRTALEAVFFVPLKSGTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LLI6</accession>
    <name>Q1LLI6_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Rmet_2111</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the BI1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08990.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516824.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2111"/>
    <dbReference type="EnsemblBacteria" id="ABF08990">
        <property type="protein sequence ID" value="ABF08990"/>
        <property type="gene ID" value="Rmet_2111"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2111"/>
    <dbReference type="eggNOG" id="COG0670">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_058671_2_1_4"/>
    <dbReference type="OMA" id="SHNAYAE"/>
    <dbReference type="OrthoDB" id="1947455at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006214">
        <property type="entry name" value="Bax_inhibitor_1-related"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23291">
        <property type="entry name" value="PTHR23291"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01027">
        <property type="entry name" value="Bax1-I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="31"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="62"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="86"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="119"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="151"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="175"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="206"/>
            <end position="230"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU004379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABF08990.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="235" mass="24985" checksum="03D3F24FE558A556" modified="2006-05-30" version="1">MNNKLNTYGFGNSAASVTDVAVRNRVLRNTYWLLAISMIPTVIGAWIGVVTGFTAMIAGSPMMSAVLFLAVAFGFMFAIEKTKNSGMGVVLLLAFTFFMGLMLARILSFTLSFSNGPALIMYAFGGTAAVFGVMATIASVSKRDFSGLAKFLFVGVILLILASLANIWLQLPALMITLSVIAIGIFSAFILVDVQRVVNGGETNYVTATLAIYLDVYNVFVNLLALLGIAGGSRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="111" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LM42</accession>
    <name>Q1LM42_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Transcriptional regulator, LacI-family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Rmet_1905</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08784.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516639.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1905"/>
    <dbReference type="EnsemblBacteria" id="ABF08784">
        <property type="protein sequence ID" value="ABF08784"/>
        <property type="gene ID" value="Rmet_1905"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1905"/>
    <dbReference type="eggNOG" id="COG1609">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037628_6_1_4"/>
    <dbReference type="OMA" id="RFFAEPY"/>
    <dbReference type="OrthoDB" id="1796334at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01392">
        <property type="entry name" value="HTH_LacI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000843">
        <property type="entry name" value="HTH_LacI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046335">
        <property type="entry name" value="LacI/GalR-like_sensor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010982">
        <property type="entry name" value="Lambda_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028082">
        <property type="entry name" value="Peripla_BP_I"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00356">
        <property type="entry name" value="LacI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13377">
        <property type="entry name" value="Peripla_BP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00354">
        <property type="entry name" value="HTH_LACI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47413">
        <property type="entry name" value="SSF47413"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53822">
        <property type="entry name" value="SSF53822"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50932">
        <property type="entry name" value="HTH_LACI_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH lacI-type" evidence="4">
        <location>
            <begin position="21"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABF08784.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="355" mass="38429" checksum="F717F5C1DABA60F7" modified="2006-05-30" version="1">MAHRQPVIAPPAANMPERKRLTLKDLAAEIDVHYSTVSRVMNPATRHLVADEVAARILQLAEQRGFRPNRIAAGLRTQRSGLVGVVLPDIANPVFPPILAGIESVLARDGYVPIVVNAGADKSRQRFVIDQLMGRQVEGLVLATAERDDPILDYCLKLSIPVVMVNRGEPTGCASCVVSDGLLAMQLTVDHLVKLGHRRIGHIAGPETTSTGQQRREGFLQAVKRHRLRRDDYAVVEAVSYSREAGREACAELFQALPGLTAIAAGNDLVAMGCYDYLREHGIRCPDDVSVVGHNDMPFVDALTPPLTTIRIAVHEMGEQAATLLLKKIGKPEAPVVSVSLTPELVVRGSTAAPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LMC2</accession>
    <name>Q1LMC2_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative metallodependent hydrolase, TatD family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_1825</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the metallo-dependent hydrolases superfamily. TatD-type hydrolase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08704.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516554.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1825"/>
    <dbReference type="EnsemblBacteria" id="ABF08704">
        <property type="protein sequence ID" value="ABF08704"/>
        <property type="gene ID" value="Rmet_1825"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1825"/>
    <dbReference type="eggNOG" id="COG0084">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031506_4_2_4"/>
    <dbReference type="OMA" id="PNEAPRI"/>
    <dbReference type="OrthoDB" id="915852at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004536">
        <property type="term" value="F:deoxyribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01310">
        <property type="entry name" value="TatD_DNAse"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018228">
        <property type="entry name" value="DNase_TatD-rel_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015991">
        <property type="entry name" value="Hydrolase_TatD-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001130">
        <property type="entry name" value="TatD-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01026">
        <property type="entry name" value="TatD_DNase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005902">
        <property type="entry name" value="DNase_TatD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="SSF51556"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00010">
        <property type="entry name" value="TIGR00010"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01137">
        <property type="entry name" value="TATD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF08704.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="265" mass="30173" checksum="CB979C34E14FD3C1" modified="2006-05-30" version="1">MFVDSHCHIDFPELAERLPQLLENMRTNQVSHALCISVTLEDFPRVLALAEQHPNLYASVGVHPDYEEGAEEPTLERLVTLSAHPRVVGTGETGLDYYRLNGRSIAEMEWQRERFRTHIRAARQTGKPLIIHTRSSADDTLRLMREENAGEVGGVMHCFTETWDIARQALDEGFHISFSGIVTFKSAADLQETAKKVPLDRMLIETDSPYLAPVPYRGKTNEPAWVRHVGEFIAKLRDVPVETIAEQTTDNFFRLFKHIDRNSHA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-10-07" version="67" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LMI4</accession>
    <name>Q1LMI4_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">SnoaL-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_1763</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08642.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1763"/>
    <dbReference type="EnsemblBacteria" id="ABF08642">
        <property type="protein sequence ID" value="ABF08642"/>
        <property type="gene ID" value="Rmet_1763"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1763"/>
    <dbReference type="eggNOG" id="COG3631">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_119884_2_0_4"/>
    <dbReference type="OMA" id="FKPYPGK"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032710">
        <property type="entry name" value="NTF2-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037401">
        <property type="entry name" value="SnoaL-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12680">
        <property type="entry name" value="SnoaL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54427">
        <property type="entry name" value="SSF54427"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SnoaL-like" evidence="1">
        <location>
            <begin position="21"/>
            <end position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12680"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF08642.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="149" mass="16136" checksum="BEE6511A55183F78" modified="2006-05-30" version="1">MRRHDMNAAGLSPAAAKTLATWHDLLARNAMEELDPLLSDSIVFRSPVAHTPYPGRAAIKLVLKTVNTVFKNFTYHRTFATADGLGVVLEFSAEVDGKALKGIDMLRFDQAGKIEEFEVMVRPMSGLQALGAAMGAKLASQKHVLAGQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-04-07" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LMR2</accession>
    <name>Q1LMR2_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Putative transporter subunit: membrane component of ABC superfamily</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Rmet_1683</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08564.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1683"/>
    <dbReference type="EnsemblBacteria" id="ABF08564">
        <property type="protein sequence ID" value="ABF08564"/>
        <property type="gene ID" value="Rmet_1683"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1683"/>
    <dbReference type="eggNOG" id="COG0842">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039483_8_1_4"/>
    <dbReference type="OMA" id="ANIYRLG"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043190">
        <property type="term" value="C:ATP-binding cassette (ABC) transporter complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000412">
        <property type="entry name" value="ABC_2_transport"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51012">
        <property type="entry name" value="ABC_TM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4 5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="46"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="196"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="248"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="280"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="366"/>
            <end position="386"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00442"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABF08564.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="397" mass="43372" checksum="2120CB67D1444E04" modified="2006-05-30" version="1">MDAAPNASTPAATPARADARRGTLRHLANIYRLGVKELWSLARDPIMLVLIIYTFTASVYTAATAQPDTLHMAPIAIVDEDHSPLSQQIASALFPPQFTMPQMITSSEVNAGMDAGNYTFALNIPPNFQRDVLGGRFAEIQLNVDATRMSQAFTGSGYVQQIVSGEIQTFVDRYRSTTRLPVELALRVRFNPTLTLAWFGALNQIINNITMLSIILTGAALIREREHGTIEHLLVMPVTPSEIMLSKVWSMGLVVLVAAAMSLMLIVRGALHVPIAGSETLFLCGAALLLFATTSMGIFMATLARSMPQFGMLLVLVLMPLQMLSGGNTPRESMPEIVQYIMLAAPTTHFVELSQAILFRGAGLEVVWVQFVALFVIGAAFFVISLRRFRKTISQMA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LN51</accession>
    <name>Q1LN51_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Hydrogenase transcriptional regulatory protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">hoxA</name>
        <name evidence="3" type="ordered locus">Rmet_1542</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08425.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1542"/>
    <dbReference type="EnsemblBacteria" id="ABF08425">
        <property type="protein sequence ID" value="ABF08425"/>
        <property type="gene ID" value="Rmet_1542"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1542"/>
    <dbReference type="eggNOG" id="COG2204">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_899778_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039420">
        <property type="entry name" value="WalR-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48111">
        <property type="entry name" value="PTHR48111"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Response regulatory" evidence="2">
        <location>
            <begin position="9"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="1">
        <location>
            <position position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF08425.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="309" mass="33276" checksum="C2F6CEF7042A9CB6" modified="2006-05-30" version="1">MTTKTRLPTVLVVDDEVRSQEALRRTLEEEFEVLTASGAGEARGIMERDFVQLLLCDQRMPGMSGVEFLKQVRSQWPDVVRMIISGYTDSKDIIAGINDAGIYQYILKPWQPEQLLLTLHNAAELYRLQSENQRLALELRTSEPVLKKRVHVGAAGGGDNGRFQAGGLRFAQLARHAGTNLHEAGDHGLGVAARLVLVQGSDEPVHVACGEQVRVVLGQETPDALLAAGDLEQNAITLQAPVPQVCTAPGSGIPPEPFHEGARSFLGRSGERLAGPIAAKVALNAWRCSCSVSARVPSTSKSSALSVMT</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="97" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LN70</accession>
    <name>Q1LN70_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">NAD-reducing hydrogenase diaphorase moiety small subunit</fullName>
            <ecNumber evidence="5">1.12.1.2</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">hoxU</name>
        <name evidence="5" type="ordered locus">Rmet_1523</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <dbReference type="EC" id="1.12.1.2" evidence="5"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08406.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516266.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1523"/>
    <dbReference type="EnsemblBacteria" id="ABF08406">
        <property type="protein sequence ID" value="ABF08406"/>
        <property type="gene ID" value="Rmet_1523"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1523"/>
    <dbReference type="eggNOG" id="COG3383">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000422_11_3_4"/>
    <dbReference type="OMA" id="NHVCAVC"/>
    <dbReference type="OrthoDB" id="323168at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047985">
        <property type="term" value="F:hydrogen dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008137">
        <property type="term" value="F:NADH dehydrogenase (ubiquinone) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042773">
        <property type="term" value="P:ATP synthesis coupled electron transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00207">
        <property type="entry name" value="fer2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036010">
        <property type="entry name" value="2Fe-2S_ferredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001041">
        <property type="entry name" value="2Fe-2S_ferredoxin-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016214">
        <property type="entry name" value="NAD-red_Hydgase_HoxS_gsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000283">
        <property type="entry name" value="NADH_UbQ_OxRdtase_75kDa_su_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019574">
        <property type="entry name" value="NADH_UbQ_OxRdtase_Gsu_4Fe4S-bd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10588">
        <property type="entry name" value="NADH-G_4Fe-4S_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000309">
        <property type="entry name" value="NAD_red_hyd_HoxU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00929">
        <property type="entry name" value="NADH-G_4Fe-4S_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54292">
        <property type="entry name" value="SSF54292"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51085">
        <property type="entry name" value="2FE2S_FER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51839">
        <property type="entry name" value="4FE4S_HC3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00641">
        <property type="entry name" value="COMPLEX1_75K_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00642">
        <property type="entry name" value="COMPLEX1_75K_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00643">
        <property type="entry name" value="COMPLEX1_75K_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="2" id="KW-0408">Iron</keyword>
    <keyword evidence="2" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="2Fe-2S ferredoxin-type" evidence="3">
        <location>
            <begin position="1"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S His(Cys)3-ligated-type" evidence="4">
        <location>
            <begin position="77"/>
            <end position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABF08406.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="236" mass="26332" checksum="0FACE47259E1DF76" modified="2006-05-30" version="1">MSIQITIDGITVTTEEGRTLVDVAAENGVYIPTLCYLKDKPCLGTCRVCSVKVNGNVNAACTVRVSKGLNVEVNSPEMVDMRKALVEFLFAEGNHNCPSCEKSGRCQLQAVGYEVDMMVSRFPYRFPVRVQDQASEKIWLERDRCIFCQRCVEFIRDKATGRKIFSISQRGSESRIEIDVELANAMPPEQVKEAVAICPVGTILEKRVGYDDPIGQRKYEIQSVRARALGLEGVDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LNP3</accession>
    <name>Q1LNP3_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Type IV secretion system transmembrane protein (Conjugal transfer:mating pair formation ) (Protein belongs to CMGI-2)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">trbI</name>
        <name evidence="7" type="ordered locus">Rmet_1350</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TrbI/VirB10 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08233.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516114.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1350"/>
    <dbReference type="EnsemblBacteria" id="ABF08233">
        <property type="protein sequence ID" value="ABF08233"/>
        <property type="gene ID" value="Rmet_1350"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1350"/>
    <dbReference type="eggNOG" id="COG2948">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_042657_0_0_4"/>
    <dbReference type="OMA" id="FANEHTR"/>
    <dbReference type="OrthoDB" id="1674599at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd16429">
        <property type="entry name" value="VirB10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.128.260">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005498">
        <property type="entry name" value="T4SS_VirB10/TraB/TrbI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042217">
        <property type="entry name" value="T4SS_VirB10/TrbI"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03743">
        <property type="entry name" value="TrbI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="39"/>
            <end position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010265"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABF08233.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="423" mass="44447" checksum="F8444DB8F1872B17" modified="2006-05-30" version="1">MIQDDTPDLARPQAGKVAPEALALRAQPRPVTRLNRRTLAILVGGLSVAVLGATIWSLQPQRRSAGEQPELYNVDRVSKSEGLDGLPADYSKLQPKVPELGPPLPGDLGPAIVNSQQPAVAAYAAPGHHPNDALRKEEEAAAASSVFFRSGGQGPTAAVAQATPGAPGGASALAAFDPLAAGSASTAAQPGDPTAVQNRQDQKEAFLKAGSTETRNSGNLALPASPYQVMAGTVIAGALVTGIKSDLPGDVIATVTEPVYDTATGKYLLIPQGSRILGRYNSQVSYGQSRVQVVWNRIILPDTSSLTLDNLVGTDPAGYSGLEDDVDWHWNRIVAGAVLTTLLGVGAELAAPENRQDGNRIVIAGRDSAQDGINQVGQEITRRNMNIQPTLTERPGLPVRIIVNRDLVLRPYQPLFFNRGASQ</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-11-03" modified="2022-08-03" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LNS4</accession>
    <name>HOA1_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">4-hydroxy-2-oxovalerate aldolase 1</fullName>
            <shortName evidence="1">HOA 1</shortName>
            <ecNumber evidence="1">4.1.3.39</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">4-hydroxy-2-keto-pentanoic acid aldolase 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">4-hydroxy-2-oxopentanoate aldolase 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="ordered locus">Rmet_1319</name>
    </gene>
    <organism>
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(S)-4-hydroxy-2-oxopentanoate = acetaldehyde + pyruvate</text>
            <dbReference type="Rhea" id="RHEA:22624"/>
            <dbReference type="ChEBI" id="CHEBI:15343"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:73143"/>
            <dbReference type="EC" id="4.1.3.39"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the 4-hydroxy-2-oxovalerate aldolase family.</text>
    </comment>
    <dbReference type="EC" id="4.1.3.39" evidence="1"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08202.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516085.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q1LNS4"/>
    <dbReference type="SMR" id="Q1LNS4"/>
    <dbReference type="STRING" id="266264.Rmet_1319"/>
    <dbReference type="EnsemblBacteria" id="ABF08202">
        <property type="protein sequence ID" value="ABF08202"/>
        <property type="gene ID" value="Rmet_1319"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1319"/>
    <dbReference type="eggNOG" id="COG0119">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_049173_0_0_4"/>
    <dbReference type="OMA" id="YVGGQED"/>
    <dbReference type="OrthoDB" id="840579at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008701">
        <property type="term" value="F:4-hydroxy-2-oxovalerate aldolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030145">
        <property type="term" value="F:manganese ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019439">
        <property type="term" value="P:aromatic compound catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd07943">
        <property type="entry name" value="DRE_TIM_HOA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01656">
        <property type="entry name" value="HOA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017629">
        <property type="entry name" value="4OH_2_O-val_aldolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012425">
        <property type="entry name" value="DmpG_comm"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035685">
        <property type="entry name" value="DRE_TIM_HOA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000891">
        <property type="entry name" value="PYR_CT"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07836">
        <property type="entry name" value="DmpG_comm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00682">
        <property type="entry name" value="HMGL-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03217">
        <property type="entry name" value="4OH_2_O_val_ald"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50991">
        <property type="entry name" value="PYR_CT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0058">Aromatic hydrocarbons catabolism</keyword>
    <keyword id="KW-0456">Lyase</keyword>
    <keyword id="KW-0464">Manganese</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000387890" description="4-hydroxy-2-oxovalerate aldolase 1">
        <location>
            <begin position="1"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="domain" description="Pyruvate carboxyltransferase" evidence="1">
        <location>
            <begin position="8"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="1">
        <location>
            <position position="20"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="16"/>
            <end position="17"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="17"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="170"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="199"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="199"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="201"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="290"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="1">
        <location>
            <position position="16"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01656"/>
        </source>
    </evidence>
    <sequence length="348" mass="37131" checksum="297DD6F6D1E19D02" modified="2006-05-30" version="1">MNLQGKKITVHDMTLRDGMHPKRHMMTLEQMKSIACGLDAAGVPLIEVTHGDGLGGSSVNYGFPAHSDEEYLGAVIPLMKQAKVSALLLPGIGTVDHLKMAKELGVHTIRVATHCTEADVSEQHIALARKLEMDTVGFLMMAHMNSPEGLVGQAKLMESYGANCVYITDSAGYMLPDDVRARLGAVREALKPETELGFHGHHNLAMGIANSIAAVECGATRIDAASAGLGAGAGNTPMEVLVAVCDRMGIQTGVDVWAIQDVAEDLVVPIMDFPIRIDRDSLTLGYAGVYGSFLLFAKRAERKYGVPAREILVELGRRGMVGGQEDMIEDTAITLAKARASKAQKVAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LNS5</accession>
    <name>Q1LNS5_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">4-oxalocrotonate decarboxylase (Protein belongs to CMGI-2)</fullName>
            <ecNumber evidence="1">4.1.1.77</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">dmpH</name>
        <name evidence="1" type="ordered locus">Rmet_1318</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EC" id="4.1.1.77" evidence="1"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08201.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516084.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1318"/>
    <dbReference type="EnsemblBacteria" id="ABF08201">
        <property type="protein sequence ID" value="ABF08201"/>
        <property type="gene ID" value="Rmet_1318"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1318"/>
    <dbReference type="eggNOG" id="COG3971">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_060136_4_0_4"/>
    <dbReference type="OMA" id="PDMDWDD"/>
    <dbReference type="OrthoDB" id="1314120at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047437">
        <property type="term" value="F:4-oxalocrotonate decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.850.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017630">
        <property type="entry name" value="4-oxalocrotonate_decarboxylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036663">
        <property type="entry name" value="Fumarylacetoacetase_C_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56529">
        <property type="entry name" value="SSF56529"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03218">
        <property type="entry name" value="catechol_dmpH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0456">Lyase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF08201.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="262" mass="27820" checksum="1D89B4906E9D0949" modified="2006-05-30" version="1">MKLDRTTIARLAEHLENCELHAQDTLKITDEHPEMDWDDAYAIQDEIRRRKLARGGKIVGLKAGLTSHAKMKQMGVESPVFGFMADYYAIPDGGTCKTEALIHPKVEPEIAFVTKAPLKGPGCHIGAVLAATDFVMPGIEVIDSRYRDFKFDLKSVVADNTSAARFVVGGRALPVSEVDLRIVGIVLEKNGEPVAFGAGAAVLGHPAAAIAMLANHLGARGEEIPAGSLILSGGITEAIAVRAGDAIALRVQGMGSTGLRFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="110" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LNT2</accession>
    <name>Q1LNT2_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Ferredoxin--NAD(+) reductase (FAD/NAD(P)-binding region)(Belongs to CMGI-2)</fullName>
            <ecNumber evidence="7">1.18.1.3</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Rmet_1311</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EC" id="1.18.1.3" evidence="7"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08194.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516077.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1311"/>
    <dbReference type="EnsemblBacteria" id="ABF08194">
        <property type="protein sequence ID" value="ABF08194"/>
        <property type="gene ID" value="Rmet_1311"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1311"/>
    <dbReference type="eggNOG" id="COG0543">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG0633">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003827_7_0_4"/>
    <dbReference type="OMA" id="YIELHIK"/>
    <dbReference type="OrthoDB" id="1834577at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008860">
        <property type="term" value="F:ferredoxin-NAD+ reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00207">
        <property type="entry name" value="fer2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036010">
        <property type="entry name" value="2Fe-2S_ferredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001041">
        <property type="entry name" value="2Fe-2S_ferredoxin-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006058">
        <property type="entry name" value="2Fe2S_fd_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012675">
        <property type="entry name" value="Beta-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008333">
        <property type="entry name" value="Cbr1-like_FAD-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017927">
        <property type="entry name" value="FAD-bd_FR_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039261">
        <property type="entry name" value="FNR_nucleotide-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001433">
        <property type="entry name" value="OxRdtase_FAD/NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017938">
        <property type="entry name" value="Riboflavin_synthase-like_b-brl"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00970">
        <property type="entry name" value="FAD_binding_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00111">
        <property type="entry name" value="Fer2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00175">
        <property type="entry name" value="NAD_binding_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52343">
        <property type="entry name" value="SSF52343"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54292">
        <property type="entry name" value="SSF54292"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63380">
        <property type="entry name" value="SSF63380"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00197">
        <property type="entry name" value="2FE2S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51085">
        <property type="entry name" value="2FE2S_FER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51384">
        <property type="entry name" value="FAD_FR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0001">2Fe-2S</keyword>
    <keyword evidence="3" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="2Fe-2S ferredoxin-type" evidence="5">
        <location>
            <begin position="4"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="domain" description="FAD-binding FR-type" evidence="6">
        <location>
            <begin position="102"/>
            <end position="201"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABF08194.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="340" mass="37941" checksum="ADA5176FD739D475" modified="2006-05-30" version="1">MSEHQILIEGEKNAFVQAGEDTVLRAALRAGIGFPYECNSGGCGSCKFDLLDGEAENLWPDAPGLTERDRRKGRLLACQCRAKGNIQIKVRVEADHQAQVRPKRLLAKFVESHEVTHDIREFRFVTDGPATFLAGQYAMLSVPGVTAPRAYSMSNTGNDRGEWHFQIRRVPQGKATEQLFHHLRAGDQLEIDGPYGLAFLRTEAPRDIVCVAGGSGLAPMVSIARGASQSGMLETRHLHFFYGGRTPSDICGESFLRMLRGYDERIHFYPVVSLPGEASGFQWSGETGFVHDVVRRVFGDTLSNFEFYFAGPPPMTQALQEMLMIGYCVPFEQLHFDRFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="95" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LNU3</accession>
    <name>Q1LNU3_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="12">HoxK membrane-bound (NiFe) hydrogenase small subunit (Belongs to CMGI-2)</fullName>
            <ecNumber evidence="12">1.12.99.6</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">hoxK</name>
        <name evidence="12" type="ordered locus">Rmet_1298</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="3">Heterodimer of a large and a small subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the [NiFe]/[NiFeSe] hydrogenase small subunit family.</text>
    </comment>
    <dbReference type="EC" id="1.12.99.6" evidence="12"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08183.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516065.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1298"/>
    <dbReference type="EnsemblBacteria" id="ABF08183">
        <property type="protein sequence ID" value="ABF08183"/>
        <property type="gene ID" value="Rmet_1298"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1298"/>
    <dbReference type="eggNOG" id="COG1740">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046107_0_0_4"/>
    <dbReference type="OMA" id="RSFNAHN"/>
    <dbReference type="OrthoDB" id="798037at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009375">
        <property type="term" value="C:ferredoxin hydrogenase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051538">
        <property type="term" value="F:3 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008901">
        <property type="term" value="F:ferredoxin hydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033748">
        <property type="term" value="F:hydrogenase (acceptor) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.700">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.480.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027394">
        <property type="entry name" value="Cytochrome-c3_hydrogenase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006137">
        <property type="entry name" value="NADH_UbQ_OxRdtase-like_20kDa"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037148">
        <property type="entry name" value="NiFe-Hase_small_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037024">
        <property type="entry name" value="NiFe_Hase_small_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001821">
        <property type="entry name" value="NiFe_hydrogenase_ssu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006311">
        <property type="entry name" value="TAT_signal"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019546">
        <property type="entry name" value="TAT_signal_bac_arc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30013">
        <property type="entry name" value="PTHR30013"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14720">
        <property type="entry name" value="NiFe_hyd_SSU_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01058">
        <property type="entry name" value="Oxidored_q6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000310">
        <property type="entry name" value="NiFe_hyd_ssu"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00391">
        <property type="entry name" value="hydA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01409">
        <property type="entry name" value="TAT_signal_seq"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51318">
        <property type="entry name" value="TAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0003">3Fe-4S</keyword>
    <keyword evidence="8" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="7 8" id="KW-0408">Iron</keyword>
    <keyword evidence="8" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="4 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 12" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 9" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193370" evidence="9">
        <location>
            <begin position="43"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="domain" description="Oxidored_q6" evidence="10">
        <location>
            <begin position="59"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="domain" description="NiFe_hyd_SSU_C" evidence="11">
        <location>
            <begin position="224"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="59"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="62"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="157"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="191"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="229"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="232"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="257"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="263"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="272"/>
        </location>
        <ligand>
            <name>[3Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:21137"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="291"/>
        </location>
        <ligand>
            <name>[3Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:21137"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="294"/>
        </location>
        <ligand>
            <name>[3Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:21137"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR000310-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF01058"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF14720"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ABF08183.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="364" mass="39952" checksum="0DB8E8A376CD57DF" modified="2006-05-30" version="1">METFYEIMRRKGISRRSFMKYCSLTATSLGLAPSFVPQIAHAMENKPRTPVLWLHGLECTCCTEAFIRSAHPLAKDVVLSMISLDYDDTLMAAAGHQAEAILEEIMTKYKGQYILAVEGNPPLNEDGMFCIQSGKPFIDKLKHVAKDAKAIIAWGSCASWGCVQAAKPNPTQATPIHKVITDKPIIKVPGCPPIPEVMTGVITYMLTFDRIPELDRQGRPKMFYSQRIHDKCYRRPHFDAGQFVEAWDDDSARKGYCLYKVGCKGPTTYNACSTVQWNEGTSFPIKAGHGCIGCSEDGFWDKGSFYDRLTDIHAFGIEANADQIGGTAAGVVGAAVAAHAAISVAKRVRDNNARKPDASTPANH</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LP52</accession>
    <name>Q1LP52_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Sulphate transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Rmet_1188</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 5">Membrane</location>
            <topology evidence="1 5">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the SLC26A/SulP transporter (TC 2.A.53) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08074.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515968.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1188"/>
    <dbReference type="EnsemblBacteria" id="ABF08074">
        <property type="protein sequence ID" value="ABF08074"/>
        <property type="gene ID" value="Rmet_1188"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1188"/>
    <dbReference type="eggNOG" id="COG0659">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003182_13_1_4"/>
    <dbReference type="OMA" id="PMGPSAI"/>
    <dbReference type="OrthoDB" id="751256at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008271">
        <property type="term" value="F:secondary active sulfate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.750.24">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011547">
        <property type="entry name" value="SLC26A/SulP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001902">
        <property type="entry name" value="SLC26A/SulP_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002645">
        <property type="entry name" value="STAS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036513">
        <property type="entry name" value="STAS_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11814">
        <property type="entry name" value="PTHR11814"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01740">
        <property type="entry name" value="STAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00916">
        <property type="entry name" value="Sulfate_transp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52091">
        <property type="entry name" value="SSF52091"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50801">
        <property type="entry name" value="STAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="53"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="84"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="127"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="155"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="180"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="205"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="259"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="338"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="388"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="domain" description="STAS" evidence="6">
        <location>
            <begin position="455"/>
            <end position="561"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU362052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABF08074.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="603" mass="64125" checksum="76187A2C6ACFC282" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LP91</accession>
    <name>Q1LP91_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">TRAP dicarboxylate transporter-DctM subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Rmet_1149</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell inner membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08035.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1149"/>
    <dbReference type="EnsemblBacteria" id="ABF08035">
        <property type="protein sequence ID" value="ABF08035"/>
        <property type="gene ID" value="Rmet_1149"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1149"/>
    <dbReference type="eggNOG" id="COG1593">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019824_4_1_4"/>
    <dbReference type="OMA" id="WNPVWFG"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010656">
        <property type="entry name" value="DctM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004681">
        <property type="entry name" value="TRAP_DctM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33362">
        <property type="entry name" value="PTHR33362"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06808">
        <property type="entry name" value="DctM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006066">
        <property type="entry name" value="HI0050"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00786">
        <property type="entry name" value="dctM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="53"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="87"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="144"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="174"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="221"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="248"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="279"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="364"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="400"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="domain" description="DctM" evidence="4">
        <location>
            <begin position="9"/>
            <end position="422"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004429"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF06808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABF08035.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="434" mass="45459" checksum="4EF2B1A28C2B243F" modified="2006-05-30" version="1">MTLIAIILFVVFIGLMLLGAPIGVSLGLGGLVAIGLSNLDTQMFGLLAVPQNFYAGLAKYPLLAIPMFVLVGSIFDRSGVAQRLVTFAIAIVGRGPGMLPLVAILVAMFLGGISGSGPANAAAVGGVMIAAMSRAGYPGSYSAAVVGAAAATDILIPPSVAFIIYSVLVPGASVPALFAAGMIPGILAGVALIVPAVWLARKHNMGHIEAGLPRPPFWKSLREAAWGLVAPFLILGGMRAGWFTPTEAAVVAVVYGLFVGMVIYRSIGVRDLFTIFQEAAETSAVILLVVALAGIFAYALSTLGVIDPLANAIAHSGLGEYGVLALIVLLLMTVGMFLDGISIFLIFVPLLLPIATAFHWNPVWFGVVLTLKVALGQFTPPLAVNLMVSCRIARVRMEETVPWVIWMLLAMFVAMLMVLAYPPLATWLPDYLGY</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LPB2</accession>
    <name>Q1LPB2_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Glycolate oxidase subunit GlcD</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">glcD2</name>
        <name evidence="3" type="ordered locus">Rmet_1128</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08014.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515915.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1128"/>
    <dbReference type="EnsemblBacteria" id="ABF08014">
        <property type="protein sequence ID" value="ABF08014"/>
        <property type="gene ID" value="Rmet_1128"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1128"/>
    <dbReference type="eggNOG" id="COG0277">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017779_4_1_4"/>
    <dbReference type="OMA" id="CNDNMLA"/>
    <dbReference type="OrthoDB" id="1034540at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.45.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.43.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.465.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016166">
        <property type="entry name" value="FAD-bd_PCMH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036318">
        <property type="entry name" value="FAD-bd_PCMH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016167">
        <property type="entry name" value="FAD-bd_PCMH_sub1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016169">
        <property type="entry name" value="FAD-bd_PCMH_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016164">
        <property type="entry name" value="FAD-linked_Oxase-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004113">
        <property type="entry name" value="FAD-linked_oxidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006094">
        <property type="entry name" value="Oxid_FAD_bind_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016171">
        <property type="entry name" value="Vanillyl_alc_oxidase_C-sub2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02913">
        <property type="entry name" value="FAD-oxidase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01565">
        <property type="entry name" value="FAD_binding_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55103">
        <property type="entry name" value="SSF55103"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56176">
        <property type="entry name" value="SSF56176"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51387">
        <property type="entry name" value="FAD_PCMH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0274">FAD</keyword>
    <keyword evidence="1" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD-binding PCMH-type" evidence="2">
        <location>
            <begin position="37"/>
            <end position="222"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF08014.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="474" mass="51324" checksum="2C1FECC8B3BAA488" modified="2006-05-30" version="1">MHSDSFLDLCRAAIGSQNVLTDPADKAPYLTDWRRRYTGDALAVLRPGSTEEVAAVMRACHAHKLAVVPQGGNTGLCGGATPEPGDAAARGTVVLSLQRMNRVRQVDPLNNTITVEAGVILQQLQEAAQTHGRLFPLSLAAEGSCTIGGNLSTNAGGTAVLRYGNTRELCLGLEVVNIKGEVWDGLRGLRKDNTGYDLRDLFIGAEGTLGIITAAVMKLFPAPRARVTALAAVESPRAALALLAIAQSHAGAMLTGFELMSAMCMDLVTKHYPQLRYPFTAHHPQLVLLELSDSESEEHARTIFEKMMEVAFEAGVVKDAVVAESVQQSRDFWNLREHIPLAQVEEGKNIKHDIAVPISRVADFIETTDTLLQDAFPGARMVTFGHLGDGNLHYNVSPPVGVDHDQFLSHQDAINLIVHNSVHVHNGSISAEHGLGQLKREENRRYKSEVELAMMRAIKQALDPQGLMNPGKVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="105" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LPE0</accession>
    <name>Q1LPE0_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Cysteine--tRNA ligase</fullName>
            <ecNumber evidence="11">6.1.1.16</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Cysteinyl-tRNA synthetase</fullName>
            <shortName evidence="11">CysRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11 13" type="primary">cysS</name>
        <name evidence="13" type="ordered locus">Rmet_1100</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 11">
            <text>ATP + L-cysteine + tRNA(Cys) = AMP + diphosphate + L-cysteinyl-tRNA(Cys)</text>
            <dbReference type="Rhea" id="RHEA:17773"/>
            <dbReference type="Rhea" id="RHEA-COMP:9661"/>
            <dbReference type="Rhea" id="RHEA-COMP:9679"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:35235"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78517"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.16"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="11">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="11">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3 11">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="11">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the class-I aminoacyl-tRNA synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.16" evidence="11"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07986.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515887.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1100"/>
    <dbReference type="EnsemblBacteria" id="ABF07986">
        <property type="protein sequence ID" value="ABF07986"/>
        <property type="gene ID" value="Rmet_1100"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1100"/>
    <dbReference type="eggNOG" id="COG0215">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013528_0_1_4"/>
    <dbReference type="OMA" id="AKYWMHN"/>
    <dbReference type="OrthoDB" id="952207at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004817">
        <property type="term" value="F:cysteine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
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        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006423">
        <property type="term" value="P:cysteinyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
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        <property type="entry name" value="CysRS_core"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Cys-tRNA-ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015273">
        <property type="entry name" value="Cys-tRNA-synt_Ia_DALR"/>
    </dbReference>
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        <property type="entry name" value="Cys-tRNA/MSH_ligase"/>
    </dbReference>
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        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
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        <property type="entry name" value="tRNA-synt_1_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009080">
        <property type="entry name" value="tRNAsynth_Ia_anticodon-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10890">
        <property type="entry name" value="PTHR10890"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09190">
        <property type="entry name" value="DALR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01406">
        <property type="entry name" value="tRNA-synt_1e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00983">
        <property type="entry name" value="TRNASYNTHCYS"/>
    </dbReference>
    <dbReference type="SMART" id="SM00840">
        <property type="entry name" value="DALR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47323">
        <property type="entry name" value="SSF47323"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00435">
        <property type="entry name" value="cysS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 11 13" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="8 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 11 13" id="KW-0436">Ligase</keyword>
    <keyword evidence="5 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9 11" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 11" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="DALR_2" evidence="12">
        <location>
            <begin position="342"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'HIGH' region" evidence="11">
        <location>
            <begin position="33"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'KMSKS' region" evidence="11">
        <location>
            <begin position="268"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="31"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="211"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="236"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="240"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="271"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM00840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ABF07986.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="472" mass="51648" checksum="C94C3850CA44A1F7" modified="2006-05-30" version="1">MTSALSLYNSLTRKKEPLRTGQPGRVTMYVCGPTVYNFAHIGNARPAVVFDVLARLLREQYAEVVYARNFTDVDDKINASAAAEGVPIGTITARYIDAYHEDMRALGVLIPDLEPRVTEHIPAIIALIQRLIANGNAYVEQDHVLFHVPSFPAYGQLSGRRPEDMLAGVRVDVAPYKRDPLDFVLWKPSTAEVPGWPSPWGRGRPGWHIECSAMIGQHLGSTIDIHGGGQDLIFPHHENEIAQGTCANGELYCRTWVHNSFVTVDGQKMSKSLGNVLLLRDLLGQAPGEAIRLALLSAHYRHPLDWNAQRLLTARQTLLRFYRGLKMAEHIEISPGEEPDEEVIAALNNDLNIPGALARLHVLLAELEKAGADASRSVAKARMMASGRLLGLFQQDPNSALRGLAGAAVTHGIATTADAARIDHLVAEREQARKRRDFARADALREELGAAGVLVHDTPNGSTWTLVDGTPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-10-07" version="66" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LPE9</accession>
    <name>Q1LPE9_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative diguanylate cyclase (GGDEF domain)</fullName>
            <ecNumber evidence="3">4.6.1.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_1091</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EC" id="4.6.1.-" evidence="3"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07977.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1091"/>
    <dbReference type="EnsemblBacteria" id="ABF07977">
        <property type="protein sequence ID" value="ABF07977"/>
        <property type="gene ID" value="Rmet_1091"/>
    </dbReference>
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    <dbReference type="eggNOG" id="COG3706">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_419700_0_0_4"/>
    <dbReference type="OrthoDB" id="1635706at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01949">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000160">
        <property type="entry name" value="GGDEF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029787">
        <property type="entry name" value="Nucleotide_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00990">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00267">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55073">
        <property type="entry name" value="SSF55073"/>
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    </dbReference>
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        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50887">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0456">Lyase</keyword>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="58"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="328"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="domain" description="GGDEF" evidence="2">
        <location>
            <begin position="510"/>
            <end position="644"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF07977.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="653" mass="71153" checksum="F0F118CA36A56D21" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LPM2</accession>
    <name>Q1LPM2_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Aminotransferase</fullName>
            <ecNumber evidence="4">2.6.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">tyrB</name>
        <name evidence="6" type="ordered locus">Rmet_1018</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 4">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 4">Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.6.1.-" evidence="4"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07904.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515815.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1018"/>
    <dbReference type="EnsemblBacteria" id="ABF07904">
        <property type="protein sequence ID" value="ABF07904"/>
        <property type="gene ID" value="Rmet_1018"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1018"/>
    <dbReference type="eggNOG" id="COG1448">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032440_1_2_4"/>
    <dbReference type="OMA" id="VGACTIV"/>
    <dbReference type="OrthoDB" id="417859at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008483">
        <property type="term" value="F:transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901605">
        <property type="term" value="P:alpha-amino acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009058">
        <property type="term" value="P:biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000796">
        <property type="entry name" value="Asp_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004838">
        <property type="entry name" value="NHTrfase_class1_PyrdxlP-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11879">
        <property type="entry name" value="PTHR11879"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00799">
        <property type="entry name" value="TRANSAMINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="SSF53383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00105">
        <property type="entry name" value="AA_TRANSFER_CLASS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aminotran_1_2" evidence="5">
        <location>
            <begin position="29"/>
            <end position="394"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABF07904.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="398" mass="43143" checksum="4B929039911C84EF" modified="2006-05-30" version="1">MSLFSAVEMAPRDPILGLNEAFNADTRATKVNLGVGVYFTDEGKIPLLRAVQEAEKARLTTATPRGYLPIEGIAAYDQAVQTLLFGKESPLITEGRVVTAQALGGTGALKIGADFLKRLYPNASVAISDPSWENHRALFEAAGFPVVNYAYYDAASHGLNFAGMVESLKSYPANTIVVLHACCHNPTGVDLDEAQWKQVVELVKERNLIPFLDMAYQGFAEGIEPDGAAVRLFAESGLPFFVSSSFSKSFSLYGERVGALSIVTTGKEEAQRVMSQIKRVIRTNYSNPPTHGGTVVATVLNSPELRAMWEQELAEMRDRIKLMRHALVDKLAAKGVPGDFSFVKAQRGMFSYSGLTSAQVDRLRTEHGIYAVGTGRICVAALNSRNIDAVVNAIAAVY</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="95" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LPP4</accession>
    <name>Q1LPP4_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Peptidase S8 and S53, subtilisin, kexin, sedolisin</fullName>
            <ecNumber evidence="7">3.4.21.62</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Rmet_0996</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EC" id="3.4.21.62" evidence="7"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07882.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515797.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0996"/>
    <dbReference type="MEROPS" id="S08.107"/>
    <dbReference type="EnsemblBacteria" id="ABF07882">
        <property type="protein sequence ID" value="ABF07882"/>
        <property type="gene ID" value="Rmet_0996"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0996"/>
    <dbReference type="eggNOG" id="COG1404">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011263_8_1_4"/>
    <dbReference type="OMA" id="DTGYVAH"/>
    <dbReference type="OrthoDB" id="923655at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd07496">
        <property type="entry name" value="Peptidases_S8_13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021206">
        <property type="entry name" value="MprA_tail"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000209">
        <property type="entry name" value="Peptidase_S8/S53_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036852">
        <property type="entry name" value="Peptidase_S8/S53_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022398">
        <property type="entry name" value="Peptidase_S8_His-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023828">
        <property type="entry name" value="Peptidase_S8_Ser-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015500">
        <property type="entry name" value="Peptidase_S8_subtilisin-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034176">
        <property type="entry name" value="Peptidases_S8_13"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00082">
        <property type="entry name" value="Peptidase_S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00723">
        <property type="entry name" value="SUBTILISIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52743">
        <property type="entry name" value="SSF52743"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03867">
        <property type="entry name" value="MprA_tail"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00137">
        <property type="entry name" value="SUBTILASE_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00138">
        <property type="entry name" value="SUBTILASE_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0645">Protease</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0720">Serine protease</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193308" evidence="5">
        <location>
            <begin position="34"/>
            <end position="540"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="513"/>
            <end position="531"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase_S8" evidence="6">
        <location>
            <begin position="167"/>
            <end position="462"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABF07882.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="540" mass="54436" checksum="C12D8DD4F089C549" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LPQ5</accession>
    <name>Q1LPQ5_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">N-acetyltransferase (GCN5-related)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_0985</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07871.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515788.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0985"/>
    <dbReference type="EnsemblBacteria" id="ABF07871">
        <property type="protein sequence ID" value="ABF07871"/>
        <property type="gene ID" value="Rmet_0985"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0985"/>
    <dbReference type="eggNOG" id="COG1670">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013985_1_0_4"/>
    <dbReference type="OMA" id="ELWKLWY"/>
    <dbReference type="OrthoDB" id="1374795at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008080">
        <property type="term" value="F:N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13302">
        <property type="entry name" value="Acetyltransf_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="SSF55729"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="1">
        <location>
            <begin position="16"/>
            <end position="153"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF07871.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="197" mass="22502" checksum="62951AF904D701D9" modified="2006-05-30" version="1">MSRFVQPVTLSGSHATLAPLSMDHAEGLREAAVDGELHRLWYTSVPSPEGMEAEIKRRLDLQTQDSMQPFTVLDANGRPAGMTTYMNIDGKNRRVEIGSTWYARRVQRTALNTECKLMLLRHAFETLDCIAVEFRTHWMNHQSRTAIARLGAKQDGVLRNHQRMPDGSFRDTVVFSIIASEWPTVRAHLQFQLDRPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="53" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LQK1</accession>
    <name>Q1LQK1_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_0689</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07575.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515542.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0689"/>
    <dbReference type="EnsemblBacteria" id="ABF07575">
        <property type="protein sequence ID" value="ABF07575"/>
        <property type="gene ID" value="Rmet_0689"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0689"/>
    <dbReference type="HOGENOM" id="CLU_084711_0_0_4"/>
    <dbReference type="OMA" id="RHDAVWI"/>
    <dbReference type="OrthoDB" id="1336963at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF07575.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="227" mass="25282" checksum="EA3A0A024FC5B75F" modified="2006-05-30" version="1">MLRYRMLMFKLNRLVGKNRLTGAEEISLAGQFAEMITSQEEVDRIIADLVDHENPQVRRIGLAAIRRSHRFGGQTLMQALLRRLADVEGWLRHDAVWIAQEGQLDGAELRAALRRVAGNVRLPQDAVRARDNPADGLLHAAVRARHVLDALIKKSAAAHNAALAAGGALAGANDGKPYAQGSIGQIHSAHRQLQRKMAARKLRSSTKLKFRKVEPRYAENDNRRFLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="101" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LQM2</accession>
    <name>Q1LQM2_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Rubredoxin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">rubA</name>
        <name evidence="9" type="ordered locus">Rmet_0668</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="6 7">
            <name>Fe(3+)</name>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
        </cofactor>
        <text evidence="6 7">Binds 1 Fe(3+) ion per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the rubredoxin family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07554.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0668"/>
    <dbReference type="EnsemblBacteria" id="ABF07554">
        <property type="protein sequence ID" value="ABF07554"/>
        <property type="gene ID" value="Rmet_0668"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0668"/>
    <dbReference type="eggNOG" id="COG1773">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_128747_1_1_4"/>
    <dbReference type="OMA" id="APKDMFE"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00730">
        <property type="entry name" value="rubredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024922">
        <property type="entry name" value="Rubredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024934">
        <property type="entry name" value="Rubredoxin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024935">
        <property type="entry name" value="Rubredoxin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018527">
        <property type="entry name" value="Rubredoxin_Fe_BS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00301">
        <property type="entry name" value="Rubredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000071">
        <property type="entry name" value="Rubredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00163">
        <property type="entry name" value="RUBREDOXIN"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00202">
        <property type="entry name" value="RUBREDOXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50903">
        <property type="entry name" value="RUBREDOXIN_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0249">Electron transport</keyword>
    <keyword evidence="5 6 7" id="KW-0408">Iron</keyword>
    <keyword evidence="3 6 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Rubredoxin-like" evidence="8">
        <location>
            <begin position="11"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="16"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="19"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="49"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="52"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005337"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR000071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR000071-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50903"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ABF07554.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="64" mass="7226" checksum="7CE3FC783AC31328" modified="2006-05-30" version="1">METGKSLHMEYKTWMCLICGWIYDEAAGAPEDGIAPGTKWEDVPINWTCPECGARKEDFEMVAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LQX7</accession>
    <name>Q1LQX7_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Phosphate acetyltransferase (Phosphotransacetylase)</fullName>
            <ecNumber evidence="3">2.3.1.19</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">ptb</name>
        <name evidence="3" type="ordered locus">Rmet_0563</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the phosphate acetyltransferase and butyryltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.19" evidence="3"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07449.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515425.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0563"/>
    <dbReference type="EnsemblBacteria" id="ABF07449">
        <property type="protein sequence ID" value="ABF07449"/>
        <property type="gene ID" value="Rmet_0563"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0563"/>
    <dbReference type="eggNOG" id="COG0280">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_056531_1_0_4"/>
    <dbReference type="OMA" id="NPDMPET"/>
    <dbReference type="OrthoDB" id="1301815at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050182">
        <property type="term" value="F:phosphate butyryltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012147">
        <property type="entry name" value="P_Ac_Bu_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002505">
        <property type="entry name" value="PTA_PTB"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01515">
        <property type="entry name" value="PTA_PTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000428">
        <property type="entry name" value="P_Ac_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PTA_PTB" evidence="2">
        <location>
            <begin position="86"/>
            <end position="300"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF07449.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="316" mass="33324" checksum="0E7FD1FF4283B52A" modified="2006-05-30" version="1">MPTTPSTDKYAQLLARCEGLPPIPTAVAHPCDQSSLDGALEAARLGLITPLLVGPEWRIRQVAEEHGLTLGDTPIIDTPHSHASAEAAVEAVRSGRAELLMKGSLHTDELLHAVTRSTSGLRTERRLSHVFAMDVPTYHKPLFITDAAVNIFPTLNEKADIVRNAIDLVRILGVERPKVAILSAVETVTDKIPSTIDAAALCMMSLRGQIEGGLLDGPLAFDNAISKAAAETKGIHSEVAGDPDILLVPDLEAGNMLAKQLTFLAGAEAAGIVLGARVPIILTSRADSVRARIGSCAIAVLIAHARRVATEAAVKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="113" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LQY5</accession>
    <name>Q1LQY5_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Formate dehydrogenase</fullName>
            <ecNumber evidence="7">1.17.1.9</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="primary">fdsA</name>
        <name evidence="19" type="ordered locus">Rmet_0555</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>formate + NAD(+) = CO2 + NADH</text>
            <dbReference type="Rhea" id="RHEA:15985"/>
            <dbReference type="ChEBI" id="CHEBI:15740"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.17.1.9"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>2Fe-2S cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49601"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Mo-bis(molybdopterin guanine dinucleotide)</name>
            <dbReference type="ChEBI" id="CHEBI:60539"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="4">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the prokaryotic molybdopterin-containing oxidoreductase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">In the C-terminal section; belongs to the prokaryotic molybdopterin-containing oxidoreductase family.</text>
    </comment>
    <dbReference type="EC" id="1.17.1.9" evidence="7"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07441.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515417.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0555"/>
    <dbReference type="EnsemblBacteria" id="ABF07441">
        <property type="protein sequence ID" value="ABF07441"/>
        <property type="gene ID" value="Rmet_0555"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0555"/>
    <dbReference type="eggNOG" id="COG3383">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000422_4_0_4"/>
    <dbReference type="OMA" id="DGPPCCY"/>
    <dbReference type="OrthoDB" id="323168at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009326">
        <property type="term" value="C:formate dehydrogenase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008863">
        <property type="term" value="F:formate dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043546">
        <property type="term" value="F:molybdopterin cofactor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015942">
        <property type="term" value="P:formate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00207">
        <property type="entry name" value="fer2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02753">
        <property type="entry name" value="MopB_Formate-Dh-H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036010">
        <property type="entry name" value="2Fe-2S_ferredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001041">
        <property type="entry name" value="2Fe-2S_ferredoxin-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009010">
        <property type="entry name" value="Asp_de-COase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041924">
        <property type="entry name" value="Formate_Dh-H_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006478">
        <property type="entry name" value="Formate_DH_asu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006657">
        <property type="entry name" value="MoPterin_dinucl-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006656">
        <property type="entry name" value="Mopterin_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006963">
        <property type="entry name" value="Mopterin_OxRdtase_4Fe-4S_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006655">
        <property type="entry name" value="Mopterin_OxRdtase_prok_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019574">
        <property type="entry name" value="NADH_UbQ_OxRdtase_Gsu_4Fe4S-bd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00037">
        <property type="entry name" value="Fer4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04879">
        <property type="entry name" value="Molybdop_Fe4S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00384">
        <property type="entry name" value="Molybdopterin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01568">
        <property type="entry name" value="Molydop_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10588">
        <property type="entry name" value="NADH-G_4Fe-4S_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00926">
        <property type="entry name" value="Molybdop_Fe4S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00929">
        <property type="entry name" value="NADH-G_4Fe-4S_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50692">
        <property type="entry name" value="SSF50692"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54292">
        <property type="entry name" value="SSF54292"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01591">
        <property type="entry name" value="Fdh-alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51085">
        <property type="entry name" value="2FE2S_FER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51839">
        <property type="entry name" value="4FE4S_HC3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51669">
        <property type="entry name" value="4FE4S_MOW_BIS_MGD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00932">
        <property type="entry name" value="MOLYBDOPTERIN_PROK_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0001">2Fe-2S</keyword>
    <keyword evidence="8 14" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="12 14" id="KW-0408">Iron</keyword>
    <keyword evidence="13 14" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="10 14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="19" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="2Fe-2S ferredoxin-type" evidence="15">
        <location>
            <begin position="18"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S His(Cys)3-ligated-type" evidence="18">
        <location>
            <begin position="96"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="16">
        <location>
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        <location>
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        </location>
        <ligand>
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        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
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        <location>
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        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
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        <source>
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        <source>
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        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
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    <sequence length="957" mass="104398" checksum="77FDAF31263E968C" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LQZ1</accession>
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        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
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                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
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                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
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            </authorList>
            <dbReference type="PubMed" id="20463976"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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</entry>
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            <fullName evidence="4">Putative GTPase</fullName>
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    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
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                <person name="Van Houdt R."/>
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                <person name="Monsieurs P."/>
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                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
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            <dbReference type="PubMed" id="20463976"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
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            <dbReference type="EMBL" id="ABF07180.1"/>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
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</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LRQ7</accession>
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        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
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                <person name="Van Houdt R."/>
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                <person name="Monsieurs P."/>
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                <person name="McCorkle S."/>
                <person name="Dunn J."/>
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            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
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    <dbReference type="EnsemblBacteria" id="ABF07169">
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    </dbReference>
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    <dbReference type="OrthoDB" id="1077305at2"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="Metalloenz_LuxS/M16"/>
    </dbReference>
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        <property type="entry name" value="Peptidase_M16_C"/>
    </dbReference>
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        <property type="entry name" value="Peptidase_M16_C"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <feature type="signal peptide" evidence="1">
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        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF05193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF07169.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="454" mass="48212" checksum="E9E48185C4371BD3" modified="2006-05-30" version="1">MSLSVMTIASPRLLRRLAGAAFGAAALLAGAVAHAAIPIESWTASTGAKVFFVPSPSIPMLDVNIDFDAGSRYDPPGKAGLATLTAALLDKGASAQDGQPARNEAQIADAFADTGADFGGAAGGDRGGIGLRTLTASPEREQSLRLAAQLIKSPTYPDAVVAREKQRLITAIREGDTRPGVIADKKLSKAIYPNHPYGVSATAETVGSITHDDLVKFWQDNYTAKRAVVTLIGAIDRKQAEQIAEELTRGLPAGAAPPTMPDVQMTIPASEQRIPHPAQQASVALGQPAIARGDPDYFPLLVGNYVLGGGGFSSRLTDQVREKRGLTYGVDSYFSPSKQPGPFSVSLQTKKENTNEALALVREIVAKYVAEGPTDAELRAAKDNLVNGFPLRIDSNRKLLTNVANIGWYGLPLDYLDTWTSQINKVTREQVRAAFQRHVHPDAMATVIVGGPEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="97" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LRW7</accession>
    <name>Q1LRW7_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Putative SAM-dependent methyltransferase</fullName>
            <ecNumber evidence="5">2.1.1.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Rmet_0223</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.1.1.-" evidence="5"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07109.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008641768.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0223"/>
    <dbReference type="EnsemblBacteria" id="ABF07109">
        <property type="protein sequence ID" value="ABF07109"/>
        <property type="gene ID" value="Rmet_0223"/>
    </dbReference>
    <dbReference type="GeneID" id="60822948"/>
    <dbReference type="KEGG" id="rme:Rmet_0223"/>
    <dbReference type="eggNOG" id="COG1092">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014042_0_0_4"/>
    <dbReference type="OMA" id="VMDVFDY"/>
    <dbReference type="OrthoDB" id="468283at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.130.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002478">
        <property type="entry name" value="PUA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015947">
        <property type="entry name" value="PUA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036974">
        <property type="entry name" value="PUA_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041532">
        <property type="entry name" value="RlmI_PUA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019614">
        <property type="entry name" value="SAM-dep_methyl-trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10672">
        <property type="entry name" value="Methyltrans_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17785">
        <property type="entry name" value="PUA_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00359">
        <property type="entry name" value="PUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88697">
        <property type="entry name" value="SSF88697"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 5" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="2 5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PUA" evidence="4">
        <location>
            <begin position="2"/>
            <end position="87"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABF07109.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="397" mass="43609" checksum="F1CCA0442EB576DD" modified="2006-05-30" version="1">MNVITLKPGKEKSLLRRHPWVYATGIAATEGRCEPGSTVVIRSADGRFLARGAYSPESQIRARVWTFDENEPVDHAMFKRRVSAALAHRKQWVRDTNAVRLIFGESDRLPGLIVDYYGQGERGQLVCQFNAAGVEQWKDALVQALIKETGCPNVYERSDAAVRQREGLPLVTGVLAGAEPDPELSVTEHGVRYHVDVRNGHKTGFYVDQRDNRKLVGDLAEGREVLNCFCYTGGFSLAALRGGATSVTSIDSSGEALKIAASNVTLNGFDPARATWLDADVFKTLREFRAEGRQFDLIVLDPPKFAPSAQHIDRAARAYKEINLVGMQLLRPGGLLFTYSCSGAISMELFQKIVAGAATDARSDARILRRLSAGTDHPMSAAFPEGEYLKGLLLEKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LRY8</accession>
    <name>Q1LRY8_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Haloacetate dehalogenase H-1</fullName>
            <ecNumber evidence="2">3.8.1.3</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">dehH1</name>
        <name evidence="2" type="ordered locus">Rmet_0202</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EC" id="3.8.1.3" evidence="2"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07088.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515108.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0202"/>
    <dbReference type="ESTHER" id="ralme-q1lry8">
        <property type="family name" value="Haloacetate_dehalogenase"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF07088">
        <property type="protein sequence ID" value="ABF07088"/>
        <property type="gene ID" value="Rmet_0202"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0202"/>
    <dbReference type="eggNOG" id="COG0596">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020336_7_1_4"/>
    <dbReference type="OMA" id="LPTLDMW"/>
    <dbReference type="OrthoDB" id="1439833at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018785">
        <property type="term" value="F:haloacetate dehalogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00561">
        <property type="entry name" value="Abhydrolase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="SSF53474"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AB hydrolase-1" evidence="1">
        <location>
            <begin position="26"/>
            <end position="151"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF07088.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="292" mass="32389" checksum="D4D63030C3F92ED3" modified="2006-05-30" version="1">MFEGFTTQRIDHDGVAIHAVVGGEGPPLLLLHGHPQTHVIWHKVAGELARHFTVVATDLRGYGDSDKPAGLPDHSNYSKRTMAADQMAVMRALGFERFRVLAHDRGARVAHRLAVDHPQAVEKLVTLDIAPTLAMYAQTNEAFARAYYHWFFLIRPAPFPETLIEADPELYLRQTMGSRSAGLAPFTDEALAEYLRGLRQPGAAHGLCEDYRASAGIDLDHDRADIEAGRMIECEMLALWGADGAVGKCFQPLEEWRKVARRVTGHALPCGHYIAEEIPEQLLAEVLPFLRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LS14</accession>
    <name>Q1LS14_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Soluble lytic murein transglycosylase</fullName>
            <ecNumber evidence="5">3.2.1.147</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Rmet_0176</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EC" id="3.2.1.147" evidence="5"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07062.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515085.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0176"/>
    <dbReference type="CAZy" id="GH23">
        <property type="family name" value="Glycoside Hydrolase Family 23"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF07062">
        <property type="protein sequence ID" value="ABF07062"/>
        <property type="gene ID" value="Rmet_0176"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0176"/>
    <dbReference type="eggNOG" id="COG0741">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019016_0_0_4"/>
    <dbReference type="OMA" id="RQESAFM"/>
    <dbReference type="OrthoDB" id="859969at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042597">
        <property type="term" value="C:periplasmic space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102799">
        <property type="term" value="F:glucosinolate glucohydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019137">
        <property type="term" value="F:thioglucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008152">
        <property type="term" value="P:metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1240.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023346">
        <property type="entry name" value="Lysozyme-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037061">
        <property type="entry name" value="Lytic_TGlycoase_superhlx_L_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012289">
        <property type="entry name" value="Lytic_TGlycosylase_superhlx_L"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008939">
        <property type="entry name" value="Lytic_TGlycosylase_superhlx_U"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008258">
        <property type="entry name" value="Transglycosylase_SLT_dom_1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01464">
        <property type="entry name" value="SLT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14718">
        <property type="entry name" value="SLT_L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48435">
        <property type="entry name" value="SSF48435"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53955">
        <property type="entry name" value="SSF53955"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193404" evidence="2">
        <location>
            <begin position="30"/>
            <end position="655"/>
        </location>
    </feature>
    <feature type="domain" description="SLT_L" evidence="4">
        <location>
            <begin position="409"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="domain" description="SLT" evidence="3">
        <location>
            <begin position="487"/>
            <end position="591"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF14718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABF07062.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="655" mass="73109" checksum="58E6379B90E05957" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-12-12" modified="2022-08-03" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LS50</accession>
    <name>ARGB_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Acetylglutamate kinase</fullName>
            <ecNumber evidence="1">2.7.2.8</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">N-acetyl-L-glutamate 5-phosphotransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">NAG kinase</fullName>
            <shortName evidence="1">NAGK</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">argB</name>
        <name type="ordered locus">Rmet_0140</name>
    </gene>
    <organism>
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the ATP-dependent phosphorylation of N-acetyl-L-glutamate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + N-acetyl-L-glutamate = ADP + N-acetyl-L-glutamyl 5-phosphate</text>
            <dbReference type="Rhea" id="RHEA:14629"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:44337"/>
            <dbReference type="ChEBI" id="CHEBI:57936"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.2.8"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-arginine biosynthesis; N(2)-acetyl-L-ornithine from L-glutamate: step 2/4.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the acetylglutamate kinase family. ArgB subfamily.</text>
    </comment>
    <comment type="sequence caution" evidence="2">
        <conflict type="erroneous initiation">
            <sequence resource="EMBL-CDS" id="ABF07026" version="1"/>
        </conflict>
    </comment>
    <dbReference type="EC" id="2.7.2.8" evidence="1"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07026.1"/>
        <property type="status" value="ALT_INIT"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q1LS50"/>
    <dbReference type="SMR" id="Q1LS50"/>
    <dbReference type="STRING" id="266264.Rmet_0140"/>
    <dbReference type="EnsemblBacteria" id="ABF07026">
        <property type="protein sequence ID" value="ABF07026"/>
        <property type="gene ID" value="Rmet_0140"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0140"/>
    <dbReference type="eggNOG" id="COG0548">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_053680_0_0_4"/>
    <dbReference type="UniPathway" id="UPA00068">
        <property type="reaction ID" value="UER00107"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003991">
        <property type="term" value="F:acetylglutamate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042450">
        <property type="term" value="P:arginine biosynthetic process via ornithine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04250">
        <property type="entry name" value="AAK_NAGK-C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1160.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00082">
        <property type="entry name" value="ArgB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036393">
        <property type="entry name" value="AceGlu_kinase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004662">
        <property type="entry name" value="AcgluKinase_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037528">
        <property type="entry name" value="ArgB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001048">
        <property type="entry name" value="Asp/Glu/Uridylate_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041727">
        <property type="entry name" value="NAGK-C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00696">
        <property type="entry name" value="AA_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000728">
        <property type="entry name" value="NAGK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53633">
        <property type="entry name" value="SSF53633"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00761">
        <property type="entry name" value="argB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0055">Arginine biosynthesis</keyword>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0418">Kinase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000264741" description="Acetylglutamate kinase">
        <location>
            <begin position="1"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="82"/>
            <end position="83"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="104"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="206"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="1">
        <location>
            <position position="47"/>
        </location>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="1">
        <location>
            <position position="266"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="309" mass="33094" checksum="A63F72982A19B694" modified="2006-12-12" version="2">MSKAMNADNPEPATKSVAAIAPSLKAEILAEALPYIRKFHGKTIVVKYGGNAMTEEKLKHGFARDVILLKLVGMNPVVVHGGGPQIDEALKKVGKVGTFIQGMRVTDEETMEVVEWVLGGEVQQDIVMLINQYGGQAVGLTGKDGGLIRAKRLQMPDRENPGTFIDIGYVGDIEAINPAVVKALQDDAFIPVISPIGFSDDGQAYNINADVVAGKMAEILKAEKLVMMTNIPGVMDKKGNLLTDLSAREIEELFADGTISGGMLPKISSALDAAKSGVHSVHIIDGRIEHSLLLEILTEQAFGTMIRSH</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-10-07" version="61" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LS66</accession>
    <name>Q1LS66_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_0124</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07010.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0124"/>
    <dbReference type="EnsemblBacteria" id="ABF07010">
        <property type="protein sequence ID" value="ABF07010"/>
        <property type="gene ID" value="Rmet_0124"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0124"/>
    <dbReference type="HOGENOM" id="CLU_1903187_0_0_4"/>
    <dbReference type="OMA" id="KPANLAW"/>
    <dbReference type="OrthoDB" id="1934777at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF07010.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="132" mass="14148" checksum="2EF2736F23C3A5BA" modified="2006-05-30" version="1">MHTESCTLSRLRARGPSLWAVLWLVACLLLAQHAGLVHRVLHGGLPNASQPALTVASDDATHTEPFSLKLGLHSCVLFDGATVADIHVGSFQLPLLAFGKPVKPANLAWRWPDLPSAQPFRSRAPPAAFAFA</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-03-23" modified="2020-10-07" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>D3DXN4</accession>
    <name>D3DXN4_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_6437</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ADC45054.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_6437"/>
    <dbReference type="EnsemblBacteria" id="ADC45054">
        <property type="protein sequence ID" value="ADC45054"/>
        <property type="gene ID" value="Rmet_6437"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6437"/>
    <dbReference type="HOGENOM" id="CLU_2668422_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADC45054.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="75" mass="8460" checksum="CB959506D1852CD9" modified="2010-03-23" version="1">MCCAARLDGYLPRLGDGRMAPDCTETRVIMRNKSNLQILTVHKQGKRGYNRPLASTVDHCTVGTRESHKFAVQER</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-03-23" modified="2020-10-07" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>D3DXQ4</accession>
    <name>D3DXQ4_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_6459</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ADC45074.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_6459"/>
    <dbReference type="EnsemblBacteria" id="ADC45074">
        <property type="protein sequence ID" value="ADC45074"/>
        <property type="gene ID" value="Rmet_6459"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6459"/>
    <dbReference type="HOGENOM" id="CLU_2993477_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADC45074.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="57" mass="6224" checksum="3F5156141FB99760" modified="2010-03-23" version="1">MPPLRPEIGEIAADGAHNTLQSGAMKMGGKWCRSVMADFTIELMNFIPETIASNRCD</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-03-23" modified="2020-10-07" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>D3DXS6</accession>
    <name>D3DXS6_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_6488</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ADC45096.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_6488"/>
    <dbReference type="EnsemblBacteria" id="ADC45096">
        <property type="protein sequence ID" value="ADC45096"/>
        <property type="gene ID" value="Rmet_6488"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6488"/>
    <dbReference type="HOGENOM" id="CLU_3238584_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADC45096.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="43" mass="4266" checksum="3638E4B9F5886FBC" modified="2010-03-23" version="1">MKAAIGFTFACWLSGAILILVDSTAAIALSGALALAGLDLFAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-03-23" modified="2021-06-02" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>D3DXZ8</accession>
    <name>D3DXZ8_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_6563</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ADC45168.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADC45168">
        <property type="protein sequence ID" value="ADC45168"/>
        <property type="gene ID" value="Rmet_6563"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6563"/>
    <dbReference type="HOGENOM" id="CLU_2957379_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="29"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="44"/>
            <end position="59"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADC45168.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="59" mass="6603" checksum="05C4795350A45687" modified="2010-03-23" version="1">MSTRGTASDEATVEPPLLDTLRFRPEVRLRPVNHGPQPHIPLEPLGHLRDRGADSNLLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-03-23" modified="2020-10-07" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>D3DYH3</accession>
    <name>D3DYH3_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_6765</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ADC45343.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_6765"/>
    <dbReference type="EnsemblBacteria" id="ADC45343">
        <property type="protein sequence ID" value="ADC45343"/>
        <property type="gene ID" value="Rmet_6765"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6765"/>
    <dbReference type="HOGENOM" id="CLU_2571336_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADC45343.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="81" mass="9749" checksum="D7432CDF2ED83453" modified="2010-03-23" version="1">MITKGNRKQYAAEAQQSLYFYALRCDFDDFAAQQRRLLVEQGNVRIQRAACRFQRFNTFPWRCCCAWHRPMCRFCRISTTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="54" xmlns="http://uniprot.org/uniprot">
    <accession>Q1L9K5</accession>
    <name>Q1L9K5_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_6312</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2 3">
        <name>pMOL28</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid pMOL28</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000355">
        <property type="protein sequence ID" value="ABF13171.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008649469.1">
        <property type="nucleotide sequence ID" value="NC_007972.2"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF13171">
        <property type="protein sequence ID" value="ABF13171"/>
        <property type="gene ID" value="Rmet_6312"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6312"/>
    <dbReference type="HOGENOM" id="CLU_1358314_0_0_4"/>
    <dbReference type="OMA" id="WVHNFWR"/>
    <dbReference type="OrthoDB" id="1360689at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid pMOL28"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022266">
        <property type="entry name" value="Integr_conj_elem_PFL4697-type"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14348">
        <property type="entry name" value="DUF4400"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="110"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="152"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="182"/>
            <end position="199"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF13171.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="205" mass="22159" checksum="C6AF8EB074C6AB02" modified="2006-05-30" version="1">MASSRFASHVRLWLIISPLMICTLAPFIQDQTAFEISRSERASVEGVLGTEKADIAVASANARFREWFIDSGAVKASFAGSDAPTAFSDGGASTFGRGWMQHFWLTIYRALYRSAVAHHWLLGATVLILAIFNDGSVSRKIHAASAGFANPVSFHVAAHGLMLCFGIGASALLLPISLLANWWTYVVCLVGLLCWRLAASFHVGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="69" xmlns="http://uniprot.org/uniprot">
    <accession>Q1L9R3</accession>
    <name>Q1L9R3_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative integrase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_6254</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3 4">
        <name>pMOL28</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid pMOL28</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000355">
        <property type="protein sequence ID" value="ABF13113.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF13113">
        <property type="protein sequence ID" value="ABF13113"/>
        <property type="gene ID" value="Rmet_6254"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6254"/>
    <dbReference type="HOGENOM" id="CLU_025122_3_0_4"/>
    <dbReference type="OMA" id="GTGHSWC"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid pMOL28"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.443.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011010">
        <property type="entry name" value="DNA_brk_join_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013762">
        <property type="entry name" value="Integrase-like_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002104">
        <property type="entry name" value="Integrase_catalytic"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00589">
        <property type="entry name" value="Phage_integrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56349">
        <property type="entry name" value="SSF56349"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51898">
        <property type="entry name" value="TYR_RECOMBINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tyr recombinase" evidence="2">
        <location>
            <begin position="234"/>
            <end position="469"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF13113.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="616" mass="68334" checksum="375ACE92F8FFAFED" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-12-02" version="67" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LA07</accession>
    <name>Q1LA07_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Antitoxin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">stbD</name>
        <name evidence="3" type="ordered locus">Rmet_6160</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3 4">
        <name>pMOL30</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid pMOL30</plasmid>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Antitoxin component of a type II toxin-antitoxin (TA) system.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the phD/YefM antitoxin family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000354">
        <property type="protein sequence ID" value="ABF13019.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF13019">
        <property type="protein sequence ID" value="ABF13019"/>
        <property type="gene ID" value="Rmet_6160"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6160"/>
    <dbReference type="HOGENOM" id="CLU_155837_1_0_4"/>
    <dbReference type="OrthoDB" id="2047723at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid pMOL30"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006442">
        <property type="entry name" value="Antitoxin_Phd/YefM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036165">
        <property type="entry name" value="YefM-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02604">
        <property type="entry name" value="PhdYeFM_antitox"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143120">
        <property type="entry name" value="SSF143120"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU362080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF13019.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="91" mass="10404" checksum="9D9680C65CC84D80" modified="2006-05-30" version="1">MSRIDPMKSMALSVVRADFEKTIVSVCKNSEPLVIKRRRGAPVVLMSLQAYESIQETLHLLGTEKNATRLRESITEFRASQPRRKGTRQYP</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-10-07" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LAD7</accession>
    <name>Q1LAD7_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_6030</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="1 2">
        <name>pMOL30</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid pMOL30</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000354">
        <property type="protein sequence ID" value="ABF12889.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF12889">
        <property type="protein sequence ID" value="ABF12889"/>
        <property type="gene ID" value="Rmet_6030"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6030"/>
    <dbReference type="HOGENOM" id="CLU_839053_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid pMOL30"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF12889.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="331" mass="35546" checksum="BE9FECC7266D8E3A" modified="2006-05-30" version="1">MRQVTQADFLARALEVHGDRYDYSRAIYSGSNSKLTIVCRTHGPFEQTPSNHYKGKGCRACAGVQPYTLQSFVDRARSLHGDRYGYAEASLVNALTPLTIRCSVHGPFEQTPRRHLSGSGCPKCAITLGADRQRGTADSFIAKAHSVHGERYDYSRVAYAATHTKVVIVCPEHGPFEQTPAAHQRGAGCPACAGVARLDTSSFVARARAIHGQRFGYPAATANLSSTSRPICFTVGRNAWSGLRVCSTGSPEEARAIASASSFVDASVTAGSEARAATAMWAQTARMLILFICMGRASRHSTFLPIRATLRGQGNLQDVCGARACASRLNC</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="62" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LAV9</accession>
    <name>Q1LAV9_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_5858</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12717.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518066.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5858"/>
    <dbReference type="EnsemblBacteria" id="ABF12717">
        <property type="protein sequence ID" value="ABF12717"/>
        <property type="gene ID" value="Rmet_5858"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5858"/>
    <dbReference type="eggNOG" id="ENOG502ZBP5">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_568303_0_0_4"/>
    <dbReference type="OMA" id="WILATAM"/>
    <dbReference type="OrthoDB" id="1834773at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="36"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="61"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="100"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="142"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="183"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="214"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="239"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="262"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="379"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="411"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="434"/>
            <end position="453"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF12717.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="479" mass="54547" checksum="2A2DE6DB3F7B5605" modified="2006-05-30" version="1">MRLQRNFVWDTLLAATLLLIVGYCHAISHMDSPMLLLSTLMLTAMVIIHWLLHVRERVPSLANSSVLLFETLFLVVAPSVQRAYYEWHMVNTATLEESLAVSTNLLCTLFVMTYVATRYYMQRRDQHRSRTRRPRPPTFVHTLRLNAPRTFVLLGVAAVAAMLSWQTIDLRAQSEVIEATPQYLLVQKYLCFMPVPMLLMLVNVKGRKLLRQPFWLLVTLFVLACVLITQNPLIEKRNAIGPIYLSLLLMLMPWFSRTPRRLVSTLVLLTGVFFPLLSIFTHTDLSAWQPSEILDPWLYFSHFLDLHYDAWANIATVIEMVNRDGLSYGHQLAGSLLFFVPHTLWETKPLATGIEIGQFLMAYYKLDFDNLSAPLIAEAYIDFFAFGVIAYAIALAFIVRLLDKLCRSGGIMALPAGIYFSIFLIFALRGALMIAMAYGSGALMAFLTIGWFVSGKRRLHTRRSRNVRPLGPTPVRPAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="61" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LAY5</accession>
    <name>Q1LAY5_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_5832</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12691.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5832"/>
    <dbReference type="EnsemblBacteria" id="ABF12691">
        <property type="protein sequence ID" value="ABF12691"/>
        <property type="gene ID" value="Rmet_5832"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5832"/>
    <dbReference type="eggNOG" id="COG2197">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1493853_0_0_4"/>
    <dbReference type="OMA" id="VWAGGQC"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF12691.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="197" mass="21150" checksum="817D5A5CA469CF81" modified="2006-05-30" version="1">MLRDDADEMLISTSMTTYLIARNEMMCRILARRMELQDVPQPVRWHCPEWLPNGATARVSQHTIDNPDEAEPLPPGGVDLVVVDCSAEDDPHELLDRVHAQLAPRRWLVLSDALDAALIGHAAKLGAGGCQAVPAPVDLVCAAAALVWAGGQCFPREALAMSHVRPVPPPATLADISSTPVLPPHTRVYPEGSPAIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="59" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LB30</accession>
    <name>Q1LB30_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_5787</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12646.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011520185.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5787"/>
    <dbReference type="EnsemblBacteria" id="ABF12646">
        <property type="protein sequence ID" value="ABF12646"/>
        <property type="gene ID" value="Rmet_5787"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5787"/>
    <dbReference type="eggNOG" id="ENOG5034BUB">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_183563_0_0_4"/>
    <dbReference type="OMA" id="IAHADMS"/>
    <dbReference type="OrthoDB" id="1977187at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004192978" evidence="1">
        <location>
            <begin position="22"/>
            <end position="82"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF12646.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="82" mass="8345" checksum="7E1ACD821C752B5D" modified="2006-05-30" version="1">MKKATSLVAIVLIAFSTLANASVAEGAREFKADVKAAGKKTGHAVHETAIAIGDGAKAAGHAVADASKRGYHSTKKFVTGHE</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="107" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LB34</accession>
    <name>Q1LB34_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Sodium:solute transporter (Symporter)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">Rmet_5783</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="12">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="12">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the sodium:solute symporter (SSF) (TC 2.A.21) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12642.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011520181.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5783"/>
    <dbReference type="EnsemblBacteria" id="ABF12642">
        <property type="protein sequence ID" value="ABF12642"/>
        <property type="gene ID" value="Rmet_5783"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5783"/>
    <dbReference type="eggNOG" id="COG4147">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018808_8_3_4"/>
    <dbReference type="OMA" id="LEYWMAV"/>
    <dbReference type="OrthoDB" id="278483at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015293">
        <property type="term" value="F:symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006814">
        <property type="term" value="P:sodium ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1730.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038377">
        <property type="entry name" value="Na/Glc_symporter_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001734">
        <property type="entry name" value="Na/solute_symporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018212">
        <property type="entry name" value="Na/solute_symporter_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00474">
        <property type="entry name" value="SSF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00813">
        <property type="entry name" value="sss"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00456">
        <property type="entry name" value="NA_SOLUT_SYMP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50283">
        <property type="entry name" value="NA_SOLUT_SYMP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0406">Ion transport</keyword>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-0614">Plasmid</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0732">Signal</keyword>
    <keyword evidence="7" id="KW-0915">Sodium</keyword>
    <keyword evidence="7" id="KW-0739">Sodium transport</keyword>
    <keyword evidence="4" id="KW-0769">Symport</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193259" evidence="10">
        <location>
            <begin position="22"/>
            <end position="553"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="37"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="78"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="107"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="151"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="184"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="215"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="265"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="301"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="358"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="408"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="433"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="469"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="500"/>
            <end position="521"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU362091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ABF12642.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="553" mass="58482" checksum="E0CA933DD803A5A6" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="95" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LBU3</accession>
    <name>Q1LBU3_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">3-oxoacyl-(Acyl-carrier-protein) reductase (3-ketoacyl-acyl carrier protein reductase)</fullName>
            <ecNumber evidence="1">1.1.1.100</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_5524</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EC" id="1.1.1.100" evidence="1"/>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12383.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519927.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5524"/>
    <dbReference type="EnsemblBacteria" id="ABF12383">
        <property type="protein sequence ID" value="ABF12383"/>
        <property type="gene ID" value="Rmet_5524"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5524"/>
    <dbReference type="eggNOG" id="COG1028">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010194_1_3_4"/>
    <dbReference type="OMA" id="NMLPIPW"/>
    <dbReference type="OrthoDB" id="1601931at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004316">
        <property type="term" value="F:3-oxoacyl-[acyl-carrier-protein] reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00061">
        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF12383.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="257" mass="26932" checksum="A7E7BD4E0130A257" modified="2006-05-30" version="1">MSQIVSLEGRTIVVTGAAQGIGKAVVELGIELGANVVAVDLNGETLNAALAPLPAERVMAVAGSVADSALATRAVADAVSRFGAVHGLVNNAGIIRPAMIDKMTDQQWTDVIDVHLSGSFYWTRAVGQHMVSRAKDGDKTGGSIVNISSDAGRKGSIGQINYAAAKAGMLGMTMTAAREWGRYNIRSNSVCFGVVETPMTEVVRGEKFRDGMLAQIPLGRWSTPEEVVKTVCFLLSDGSSYVTGQHLGVNGGFHISL</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="59" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LC07</accession>
    <name>Q1LC07_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_5460</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12319.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5460"/>
    <dbReference type="EnsemblBacteria" id="ABF12319">
        <property type="protein sequence ID" value="ABF12319"/>
        <property type="gene ID" value="Rmet_5460"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5460"/>
    <dbReference type="eggNOG" id="ENOG503445S">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1132885_0_0_4"/>
    <dbReference type="OMA" id="PRGMQVW"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF12319.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="245" mass="27611" checksum="8C971EAB2A7C4ABE" modified="2006-05-30" version="1">MRQLGSSCSFMGQQSSPSQDAVRREKLGSSADRLPQPFYRFFEPAEQGFVRSGTVRGGHAMTTMPTLYLAFDGVLHPNLVSFHGGCAPRLRAGGHTLFENNTILEQLMDACPSTVVVLHNWWVQIVGYQGTLRLLPESVRSHVIGSTWRECRGPRARVTNNRSRRDWLQNDLVRRQPEHPVLVDCDARQVVPQLLDSACIVDNWRGLTEPGAWERLVTLLTTRPEQTQISGYSLDPRHLKERVAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="108" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LCE9</accession>
    <name>Q1LCE9_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Two component transcriptional regulator, LuxR family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Rmet_5318</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="6">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="6">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12177.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5318"/>
    <dbReference type="EnsemblBacteria" id="ABF12177">
        <property type="protein sequence ID" value="ABF12177"/>
        <property type="gene ID" value="Rmet_5318"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5318"/>
    <dbReference type="eggNOG" id="COG4566">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_90_4_4"/>
    <dbReference type="OMA" id="HRGHAMR"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06170">
        <property type="entry name" value="LuxR_C_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000792">
        <property type="entry name" value="Tscrpt_reg_LuxR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00196">
        <property type="entry name" value="GerE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00038">
        <property type="entry name" value="HTHLUXR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00421">
        <property type="entry name" value="HTH_LUXR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00622">
        <property type="entry name" value="HTH_LUXR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50043">
        <property type="entry name" value="HTH_LUXR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="5" id="KW-0614">Plasmid</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Response regulatory" evidence="4">
        <location>
            <begin position="45"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="domain" description="HTH luxR-type" evidence="3">
        <location>
            <begin position="175"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="2">
        <location>
            <position position="94"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABF12177.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="247" mass="26731" checksum="EC6A0A4E6F0CE307" modified="2006-05-30" version="1">MPGRIAWPLAFQGKAPIIPLPGTNAPRIAMTRDRIEEGGESEESAVLVVDDDQDVRVALSSLFRSAGLAAESFASAEELLAHGIPDRPTCIVLDVHLHGASGLDLQATLNRAGRHASIVFISGRGDVPMTVAAMKAGAVNFIAKPFRDQDLLDAVDEALQRDRAQREAEAQSRQLRDHFDTLTGREQEVMQLAARGLMNKQIADDLGISEATVKIYRGHAMRKMQARTFAELVIMAQSLGLVQDDAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="69" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LCI1</accession>
    <name>Q1LCI1_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_5286</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12145.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008644170.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5286"/>
    <dbReference type="EnsemblBacteria" id="ABF12145">
        <property type="protein sequence ID" value="ABF12145"/>
        <property type="gene ID" value="Rmet_5286"/>
    </dbReference>
    <dbReference type="GeneID" id="60826513"/>
    <dbReference type="KEGG" id="rme:Rmet_5286"/>
    <dbReference type="eggNOG" id="COG3246">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_065536_2_0_4"/>
    <dbReference type="OMA" id="GMDQANI"/>
    <dbReference type="OrthoDB" id="1892901at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019475">
        <property type="term" value="P:L-lysine catabolic process to acetate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008567">
        <property type="entry name" value="KCE_family"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37418">
        <property type="entry name" value="PTHR37418"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05853">
        <property type="entry name" value="BKACE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="334"/>
            <end position="355"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF12145.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="355" mass="39501" checksum="A576118B90A96358" modified="2006-05-30" version="1">MHFLDGHLYPENQQPLIITAAPYAPGWIPEDFPEDIPVTMEEQIQKAVDCYEAGARVLHLHVREANGKGSKRLSMFNELIAGVRAAVPDMVIQVGGSISFAPEEGQDAKWLSDDTRHMLAELDPKPDQVTVTVNTSQMNVTEHAGLDDFKGVSRGFPHLYKTYKDMVVPSNPSWAEEHIRRLTAAGIQSEFQCYNINSFETIERMIRRGVYKGPLVMNWVAISGGMDQANIFNLANMLRAVPDGAVVTVESSVLNVLPINMIGIALGLHVRCGIEDVLWNQSRTGKMSSVEQIKQLVRISREFGRPIATAKQTREILKLGVFYDTVEETLQANGFSPNRNGANQGFLRKQENLPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LCZ9</accession>
    <name>Q1LCZ9_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Outer membrane protein (Porin) OmpC family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Rmet_5118</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="8">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="8">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11977.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519524.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5118"/>
    <dbReference type="EnsemblBacteria" id="ABF11977">
        <property type="protein sequence ID" value="ABF11977"/>
        <property type="gene ID" value="Rmet_5118"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5118"/>
    <dbReference type="eggNOG" id="COG3203">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038238_2_0_4"/>
    <dbReference type="OMA" id="KNEWTAG"/>
    <dbReference type="OrthoDB" id="1064531at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046930">
        <property type="term" value="C:pore complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015288">
        <property type="term" value="F:porin activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006811">
        <property type="term" value="P:ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00342">
        <property type="entry name" value="gram_neg_porins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.160.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033900">
        <property type="entry name" value="Gram_neg_porin_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023614">
        <property type="entry name" value="Porin_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002299">
        <property type="entry name" value="Porin_Neis"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13609">
        <property type="entry name" value="Porin_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00184">
        <property type="entry name" value="NEISSPPORIN"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0406">Ion transport</keyword>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-0626">Porin</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1134">Transmembrane beta strand</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193014" evidence="5">
        <location>
            <begin position="22"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="domain" description="Porin_4" evidence="6">
        <location>
            <begin position="8"/>
            <end position="319"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF13609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABF11977.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="355" mass="37809" checksum="E34A3E7155891226" modified="2006-05-30" version="1">MNVRHGVAMATLGMIAGTASAQSSVTLYGVADVGIEYLSNVPSANGGASQVRMASGNMSTSRWGIRGVEDLGGGLKAIFDLESGISLDTGAQNNRTRLFDRTSIVGLSSKYGTVTLGRQTTPLYDTAIQLDPMGFAPRYSLYKSDDVLAGRADNSIKYRGTFNGLTASGLYSFSRTGGGEVPGNYTLDRNLGISLSYEVSSFAIGAVYDEYQGSTLATAERKDRRMLVGASYAFGPAKAFIGYRWYNGNVGAMPTDRSDLYWAGLRYSVTPALKLTGAAYYTNMRNAGADPIMFVASADYAFSKRTDVYMNAGYALNRGNSQLGMNGFNTPSGSPTNVVPGKDQTGLVVGVRHKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="92" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LD15</accession>
    <name>Q1LD15_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative alcohol dehydrogenase, zinc-binding</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_5102</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11961.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519507.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5102"/>
    <dbReference type="EnsemblBacteria" id="ABF11961">
        <property type="protein sequence ID" value="ABF11961"/>
        <property type="gene ID" value="Rmet_5102"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5102"/>
    <dbReference type="eggNOG" id="COG1063">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026673_0_0_4"/>
    <dbReference type="OMA" id="LENTWAL"/>
    <dbReference type="OrthoDB" id="884088at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013149">
        <property type="entry name" value="ADH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013154">
        <property type="entry name" value="ADH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020843">
        <property type="entry name" value="PKS_ER"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08240">
        <property type="entry name" value="ADH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00107">
        <property type="entry name" value="ADH_zinc_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00829">
        <property type="entry name" value="PKS_ER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="SSF50129"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PKS_ER" evidence="1">
        <location>
            <begin position="10"/>
            <end position="353"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF11961.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="358" mass="37246" checksum="B056661037C3838D" modified="2006-05-30" version="1">MKAWMLDQPGQPLVLRDIDTPHARPGAVLVRMEAVPLLSYTRDYLNGALPYAYPPGPFTPGTNGVGTIIETGAGVHHFRAGQRVAVNPYLTSTESVDAPAEILIGLTGISADSGPLLQDFPHGTLREVAEFPAAALVPLDGLDRLSSDQLATLSKFAIPFGGLRRGRLVAGETVAINGATGAFGSAAVLAALAMGASTVLALGRRAGPLESLARMGDGRVVPVVMTGDEGVDSAAIRSAASGGVDLAFDMVGQATSPNSTLAALLSLKRRGRMVLMGSMTVPLPLPYGAMLRNGWELIGHFMYASADYRALVAMVRAGQLSLDAIHVKRFPFDAIEQAIDAAGTLAGLEAAVVTFPHD</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-12-02" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LD24</accession>
    <name>Q1LD24_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">N-acyl-D-amino-acid deacylase</fullName>
            <ecNumber evidence="2">3.5.1.81</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_5093</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EC" id="3.5.1.81" evidence="2"/>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11952.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5093"/>
    <dbReference type="EnsemblBacteria" id="ABF11952">
        <property type="protein sequence ID" value="ABF11952"/>
        <property type="gene ID" value="Rmet_5093"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5093"/>
    <dbReference type="eggNOG" id="COG3653">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016107_2_0_4"/>
    <dbReference type="OMA" id="ETAVWKM"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047420">
        <property type="term" value="F:N-acyl-D-amino-acid deacylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013108">
        <property type="entry name" value="Amidohydro_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023100">
        <property type="entry name" value="D-aminoacylase_insert_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011059">
        <property type="entry name" value="Metal-dep_hydrolase_composite"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07969">
        <property type="entry name" value="Amidohydro_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51338">
        <property type="entry name" value="SSF51338"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="SSF51556"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Amidohydro_3" evidence="1">
        <location>
            <begin position="60"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="domain" description="Amidohydro_3" evidence="1">
        <location>
            <begin position="355"/>
            <end position="473"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF07969"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF11952.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="494" mass="53276" checksum="15850446E6078166" modified="2006-05-30" version="1">MPLQETRSMAEQASNHHDLLIRGGTVIDGSKAPRFTADIAVRDGRIAAIGDLAGHTAERTLDAAGRIVAPGFIDSHTHDDQAVLSQAAMPFKISQGVTTVVAGNCGISAAPLRADMDLPMPLGLIDAPPEGRFTTFAAYLDALRATPSSVNVAAMVGHSTLRAVTMSALDRPANDDEIAAMQALVEEAMQAGAIGLSTGTFYPPAIKATTEEIIEVCRPLSARKALYVTHMRNESDQVMEALEETFQIGRALEVPVVVSHHKVQNTPNFGRSRVTLPFIQETMQHQCVSLDCYPYTAGSTMIRTEPAMLEGRVRIASSVPHPECTGRDLDDIAREWGVAKQEAARRLQPGTAIYFLMDEADVQRILAFDETMIGSDGIPVGENPHPRLWGTFPRVLGHYCREVGLFPLETAVWKMTGLTARNFGLHGRGTLAVGNHADMVIFDAGTIRDAADYDTPTRPAEGIDTVIVNGAITWHGGQHTGARNGQVIKRRHAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LD45</accession>
    <name>Q1LD45_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">O-methyltransferase, family 2</fullName>
            <ecNumber evidence="6">2.1.1.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Rmet_5070</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="7">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="7">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the class I-like SAM-binding methyltransferase superfamily. Cation-independent O-methyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.-" evidence="6"/>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11931.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519480.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5070"/>
    <dbReference type="EnsemblBacteria" id="ABF11931">
        <property type="protein sequence ID" value="ABF11931"/>
        <property type="gene ID" value="Rmet_5070"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5070"/>
    <dbReference type="eggNOG" id="COG0500">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005533_12_0_4"/>
    <dbReference type="OMA" id="CTEPWTW"/>
    <dbReference type="OrthoDB" id="1213908at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008171">
        <property type="term" value="F:O-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031725">
        <property type="entry name" value="ASMT_dimerisation"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016461">
        <property type="entry name" value="COMT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001077">
        <property type="entry name" value="O_MeTrfase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16864">
        <property type="entry name" value="Dimerisation2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00891">
        <property type="entry name" value="Methyltransf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005739">
        <property type="entry name" value="O-mtase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51683">
        <property type="entry name" value="SAM_OMT_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="6" id="KW-0614">Plasmid</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 3" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="3 6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Dimerisation2" evidence="5">
        <location>
            <begin position="37"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="domain" description="Methyltransf_2" evidence="4">
        <location>
            <begin position="138"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="2 3">
        <location>
            <position position="278"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="228"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR005739-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00891"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF16864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABF11931.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="369" mass="40715" checksum="F30808494D8AC06D" modified="2006-05-30" version="1">MTFQMNAGAVRRFAKAMRFGAWLQSITERMTPAPFRLVQIGSAYWQSKALYVAAKLDLATVLGTATLTASALASRVGANEDALGRLMRLLAAMGIFEETAPMVFRNNKLSRCLVSDDPQSVRAMILMHNSETMSRPWFEQLEAGIRSGTPPFQLSHGEDLFDYLDHHADFDQLFSEAMNSVEALAGDGFATDLDWARFDRIIDVGGSRGTKSLAILRRHPRLTALIVDRPQVIEEAQRYWAEHHADGLERLQFQAADLFESLPAAGPKDVYLYSAVLHGFDDPTCVQALLRLREAIGNSGARAAILEIVVPEKGADISSASFDMQMFVGSRGRERTLTEWKAIIQAGGLTLEEVVRLRSLGSILVLRAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="68" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LD83</accession>
    <name>Q1LD83_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Heavy metal cation tricomponent efflux outer membrane porin CusC (CzcC-like)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">cusC</name>
        <name evidence="3" type="ordered locus">Rmet_5031</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the outer membrane factor (OMF) (TC 1.B.17) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11893.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519444.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5031"/>
    <dbReference type="EnsemblBacteria" id="ABF11893">
        <property type="protein sequence ID" value="ABF11893"/>
        <property type="gene ID" value="Rmet_5031"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5031"/>
    <dbReference type="eggNOG" id="COG1538">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012817_15_1_4"/>
    <dbReference type="OMA" id="REGMTMQ"/>
    <dbReference type="OrthoDB" id="1533572at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015562">
        <property type="term" value="F:efflux transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003423">
        <property type="entry name" value="OMP_efflux"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02321">
        <property type="entry name" value="OEP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193020" evidence="2">
        <location>
            <begin position="25"/>
            <end position="433"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF11893.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="433" mass="46978" checksum="5747000125812321" modified="2006-05-30" version="1">MSFPLRLRLALLCASALGMSVSHAASPHAVPSLSLEEAAALATTHAHDAENTRGAVEAAVQMAVAAGQLPDPVLKVGLNNVPTDGPDKFSLTRESMTMRSVSVMQEFTRSDKRRARAARFEAEAASADAQRMVGMTNVQRSAVAAWIDRWYAERAAALLASHEQPLSLTLEAANAAYRSGRGTRADVLSAQFEVQKLRDRENENQAALDTANVNLTRWIGTDASRPLGDPPALTVPDRVAQLARSHFDGVPEVAAAQRDVALAESEIRVADEARKPDVSLELMYSQRGSAYSNLASINVSIPLPWDRANRQDREVAARLAQATAARAKAEMIRRDTDAVVGARLAELRRNLERLKRYDDATLPLTNAQAEAALTAYRANAGSLQAVADANHRVIDTSLERLSLEQRTTRLWAELTFLTPLPTAQTEPANKDLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LDI0</accession>
    <name>Q1LDI0_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Putative Xanthine/uracil/vitamin C permease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Rmet_4934</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="8">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="8">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the nucleobase:cation symporter-2 (NCS2) (TC 2.A.40) family. Azg-like subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11796.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519356.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4934"/>
    <dbReference type="DNASU" id="4041796"/>
    <dbReference type="EnsemblBacteria" id="ABF11796">
        <property type="protein sequence ID" value="ABF11796"/>
        <property type="gene ID" value="Rmet_4934"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4934"/>
    <dbReference type="eggNOG" id="COG2252">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024508_0_1_4"/>
    <dbReference type="OMA" id="PDAMGMV"/>
    <dbReference type="OrthoDB" id="558559at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015205">
        <property type="term" value="F:nucleobase transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904823">
        <property type="term" value="P:purine nucleobase transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045018">
        <property type="entry name" value="Azg-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006043">
        <property type="entry name" value="NCS2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43337">
        <property type="entry name" value="PTHR43337"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00860">
        <property type="entry name" value="Xan_ur_permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0614">Plasmid</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="40"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="68"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="94"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="119"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="152"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="190"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="217"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="263"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="310"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="344"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="376"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="399"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="439"/>
            <end position="459"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABF11796.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="460" mass="46918" checksum="EDFDA052DDD5FC51" modified="2006-05-30" version="1">MIEQPYPSVADTDAAPPTAQGKIDQYFQISARGSTQRREVVAGITTFMAMVYAVFVVPGMLGKAGFDTSAVFVAVCLTTAFGSLLMGLWAKLPIAIGCAISLTAFMAFGLVLGQHLSPAVALGAVFLMGVVFTAISVTGVRTWILRNLPAGVAHGAGIGIGLFLLLIASNEVGLVIKNPGPGLPVSLGNITAFPVMMSILGLAAIFGLERRRVPGGILLVIVAISALGLVFDPSVKFTGIFALPTLSAPGHTSLIGAMDIRGALSAAVLPSVLALVMTAVFDATGTIRAVAGQANQLDATGRIHNGGRALTADSVSSIFSAFFGGAPAAAYIESTVGVAAGARTGLAAVTVGALFLAVMFFSPLAGLVPSYATAPALMYVGLLMLSSVSRLHMDDLVDALSGLVCAVFIVLTCNIVTGIMLGFCTLVIGRIVSGEWRKLNIGTVAIAVALAAFYAGGWAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="62" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LDW1</accession>
    <name>Q1LDW1_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_4803</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11665.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519230.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4803"/>
    <dbReference type="EnsemblBacteria" id="ABF11665">
        <property type="protein sequence ID" value="ABF11665"/>
        <property type="gene ID" value="Rmet_4803"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4803"/>
    <dbReference type="eggNOG" id="ENOG502ZU3U">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_092718_0_0_4"/>
    <dbReference type="OMA" id="EYYVYVE"/>
    <dbReference type="OrthoDB" id="1363501at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF11665.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="207" mass="23125" checksum="A8777B3DB76617A1" modified="2006-05-30" version="1">MTLTLAMPSWGQVLARILELRIDVSLSPGEVERELDTLHDRIRQPGDKLHDMPAVPTALPGVRLRYREADGEYYVYVEDTRYGRIAGYTVFNRLIEVSRRTDRLVRAPHTKLGPAYQRCGLATSIYGWALDGGLCLISGARQSAGANALWGTLARRHTRGYVDLRDKSMTYLGESVSETVQADLHTRMILLGQGWTLSSFADATGMR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="98" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LDY5</accession>
    <name>Q1LDY5_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Branched-chain amino acid ABC transporter, ATP-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">livG10</name>
        <name evidence="9" type="ordered locus">Rmet_4779</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="10">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="10">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11641.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519209.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4779"/>
    <dbReference type="EnsemblBacteria" id="ABF11641">
        <property type="protein sequence ID" value="ABF11641"/>
        <property type="gene ID" value="Rmet_4779"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4779"/>
    <dbReference type="eggNOG" id="COG0411">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_2_4"/>
    <dbReference type="OMA" id="FQINQLY"/>
    <dbReference type="OrthoDB" id="1179151at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032823">
        <property type="entry name" value="BCA_ABC_TP_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12399">
        <property type="entry name" value="BCA_ABC_TP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 7 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="1" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-0614">Plasmid</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-1278">Translocase</keyword>
    <feature type="domain" description="ABC transporter" evidence="8">
        <location>
            <begin position="5"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="37"/>
            <end position="44"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ABF11641.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="250" mass="26951" checksum="30D3CC8CCFB64EA7" modified="2006-05-30" version="1">MTAILRTEGLSKSWGAFAANSDVSLTFKPGARHALIGPNGAGKTTFVNLLTGALAPSAGRIWLGERDITTLPQHVRVELGMTRTFQINTLFPGLTVLESVVLAVAERTGAGRVWRRTVASQVALVDEAMAVLAMLRLEADADVETRQLPYGKQRLLEMALALATRPSILLLDEPAAGIPTGESAELFAVIAALPRDITIVFIEHDMDLVFRFAERITVLVAGRVLMEGTPAEVSTDPRVREVYLGEAAHE</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LE26</accession>
    <name>Q1LE26_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative mannose-6-phosphate isomerase (With a Cupin 2, conserved barrel)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_4738</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11600.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4738"/>
    <dbReference type="EnsemblBacteria" id="ABF11600">
        <property type="protein sequence ID" value="ABF11600"/>
        <property type="gene ID" value="Rmet_4738"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4738"/>
    <dbReference type="eggNOG" id="COG0662">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_131430_1_0_4"/>
    <dbReference type="OMA" id="PKGVEHC"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016853">
        <property type="term" value="F:isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013096">
        <property type="entry name" value="Cupin_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011051">
        <property type="entry name" value="RmlC_Cupin_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07883">
        <property type="entry name" value="Cupin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51182">
        <property type="entry name" value="SSF51182"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0413">Isomerase</keyword>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cupin_2" evidence="1">
        <location>
            <begin position="49"/>
            <end position="115"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF07883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF11600.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="141" mass="15653" checksum="D482744B2DEACD3B" modified="2006-05-30" version="1">MPQAPKHPTMHADRGQCRAIDLIDKIALIDGHWQPRVVAEMNDYQFKVVKVEGEFQWHRHVDTDETFIVLEGELRIDVRAGPTGDGSIVLRAGQMAVVPKGVEHKPCANAEVKLLLIEPRGVVNTGDGAAGERTVENDQWI</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LEC5</accession>
    <name>Q1LEC5_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">VOC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_4639</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11501.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4639"/>
    <dbReference type="EnsemblBacteria" id="ABF11501">
        <property type="protein sequence ID" value="ABF11501"/>
        <property type="gene ID" value="Rmet_4639"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4639"/>
    <dbReference type="eggNOG" id="COG0346">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046006_6_0_4"/>
    <dbReference type="OMA" id="KLCVACH"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.180.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029068">
        <property type="entry name" value="Glyas_Bleomycin-R_OHBP_Dase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004360">
        <property type="entry name" value="Glyas_Fos-R_dOase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037523">
        <property type="entry name" value="VOC"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00903">
        <property type="entry name" value="Glyoxalase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54593">
        <property type="entry name" value="SSF54593"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51819">
        <property type="entry name" value="VOC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="VOC" evidence="1">
        <location>
            <begin position="1"/>
            <end position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF11501.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="132" mass="14461" checksum="54F8E9DF339EC0F0" modified="2006-05-30" version="1">MISHVFVGVTDFARAFRFYSVIMGDLGHKLKFSEPDKPWAGWMAEGMPRPLFLIGGPYDGNPARCGNGQMVALLAPDRKSVDKAYASALATGGSCEGAPGLRPEYHPDYYGAYFRDPEGNKICVCCHDPVRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="105" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LED8</accession>
    <name>Q1LED8_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Histidine kinase</fullName>
            <ecNumber evidence="2">2.7.13.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">Rmet_4626</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="12">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="12">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.</text>
            <dbReference type="EC" id="2.7.13.3"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.7.13.3" evidence="2"/>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11488.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519064.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4626"/>
    <dbReference type="EnsemblBacteria" id="ABF11488">
        <property type="protein sequence ID" value="ABF11488"/>
        <property type="gene ID" value="Rmet_4626"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4626"/>
    <dbReference type="eggNOG" id="COG0784">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG2205">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_15_1_4"/>
    <dbReference type="OMA" id="FAMYRYW"/>
    <dbReference type="OrthoDB" id="1755994at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00082">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00088">
        <property type="entry name" value="HPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.160">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003661">
        <property type="entry name" value="HisK_dim/P"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036097">
        <property type="entry name" value="HisK_dim/P_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036641">
        <property type="entry name" value="HPT_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004358">
        <property type="entry name" value="Sig_transdc_His_kin-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008207">
        <property type="entry name" value="Sig_transdc_His_kin_Hpt_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00512">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01627">
        <property type="entry name" value="Hpt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00344">
        <property type="entry name" value="BCTRLSENSOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00388">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47226">
        <property type="entry name" value="SSF47226"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47384">
        <property type="entry name" value="SSF47384"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="SSF55874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50894">
        <property type="entry name" value="HPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="11" id="KW-0418">Kinase</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="11" id="KW-0614">Plasmid</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="37"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="8">
        <location>
            <begin position="395"/>
            <end position="611"/>
        </location>
    </feature>
    <feature type="domain" description="Response regulatory" evidence="9">
        <location>
            <begin position="752"/>
            <end position="866"/>
        </location>
    </feature>
    <feature type="domain" description="HPt" evidence="10">
        <location>
            <begin position="885"/>
            <end position="983"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="5">
        <location>
            <position position="801"/>
        </location>
    </feature>
    <feature type="modified residue" description="Phosphohistidine" evidence="4">
        <location>
            <position position="924"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ABF11488.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="983" mass="105490" checksum="D2B335177A67FE2E" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="104" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LEN9</accession>
    <name>Q1LEN9_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transcriptional regulator, AraC family with amidase-like domain</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_4522</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11387.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518980.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4522"/>
    <dbReference type="EnsemblBacteria" id="ABF11387">
        <property type="protein sequence ID" value="ABF11387"/>
        <property type="gene ID" value="Rmet_4522"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4522"/>
    <dbReference type="eggNOG" id="COG4977">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_59_0_4"/>
    <dbReference type="OMA" id="TTHWKSA"/>
    <dbReference type="OrthoDB" id="871215at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002818">
        <property type="entry name" value="DJ-1/PfpI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018060">
        <property type="entry name" value="HTH_AraC"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01965">
        <property type="entry name" value="DJ-1_PfpI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12833">
        <property type="entry name" value="HTH_18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00342">
        <property type="entry name" value="HTH_ARAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="SSF52317"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01124">
        <property type="entry name" value="HTH_ARAC_FAMILY_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH araC/xylS-type" evidence="1">
        <location>
            <begin position="212"/>
            <end position="310"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS01124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF11387.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="313" mass="34348" checksum="771BC7703359E866" modified="2006-05-30" version="1">MHNIGFLLTDGFQVMALATQSVFEYANLVAGEPFYRIRNYSIDGGPVVSSLGMTVETHAASARVAVDTWVLAGVGDPVAQPTPAPVLAFVSKAVPRARRTASICTGAFILAEAGLLDGRRATTHWAFARDMKRLHPDIKVEDDRIYIVDGAIWTSAGMTAGLDLALAMVEKDLGADIARSVAHKLVMHQRRSGGQSQHSELLDMAPKSDRIQSALDYARKNLSQALTVEELAETVHLSPRQFSRVFTAETGQSPAKAIENLRLEAARLMIEQSRHPLEVVAREAGFRDRRHMREAFLRGFGVPPQAVRREARE</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LEZ8</accession>
    <name>Q1LEZ8_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Acetyl-CoA acetyltransferase (Thiolase)</fullName>
            <ecNumber evidence="1">2.3.1.9</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_4413</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EC" id="2.3.1.9" evidence="1"/>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11278.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518892.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4413"/>
    <dbReference type="EnsemblBacteria" id="ABF11278">
        <property type="protein sequence ID" value="ABF11278"/>
        <property type="gene ID" value="Rmet_4413"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4413"/>
    <dbReference type="eggNOG" id="COG0183">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035425_2_1_4"/>
    <dbReference type="OMA" id="EVPYGHT"/>
    <dbReference type="OrthoDB" id="647502at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003985">
        <property type="term" value="F:acetyl-CoA C-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.47.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002155">
        <property type="entry name" value="Thiolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016039">
        <property type="entry name" value="Thiolase-like"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000429">
        <property type="entry name" value="Ac-CoA_Ac_transf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53901">
        <property type="entry name" value="SSF53901"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF11278.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="389" mass="40874" checksum="AA3A1D2F7BA76E1F" modified="2006-05-30" version="1">MSIKGKAYIVGAYEHPTRKAPDKSVAQLHAECARGALEDAGLSLSDVDGYFCAGDAPGLGATNMIDYLGLRVRHVDSTDTGGSAYLVHVSHAAQAIAAGKCNVALITLAGRPRSEGSSGTQARNWGANLPDAPFEAPFHPVTVNLYAMVAMRHMHEYGTTPEQLAWIKVAASHHAQHNPHAMLRDVVTVEDVLNSPLISDPLRKLDCCVVSDGGGALIVACPEIAAKLKRPKVRILGAGEHVKGQLGGQVDLSWSAARVSGAAAFAEAGITPADIKYASIYDSFTITVLMQLEDLGFCKKGEGGRFVADGNLISGVGRLPFNTDGGGLCNNHPANRGGITKVIEAVRQLRGEAHPAVQVKHCDLALAQGTGGYLGSRHGSATLILEREQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LFP2</accession>
    <name>Q1LFP2_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative toluene tolerance family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">ttg2</name>
        <name evidence="2" type="ordered locus">Rmet_4167</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11034.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008645112.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4167"/>
    <dbReference type="EnsemblBacteria" id="ABF11034">
        <property type="protein sequence ID" value="ABF11034"/>
        <property type="gene ID" value="Rmet_4167"/>
    </dbReference>
    <dbReference type="GeneID" id="60823252"/>
    <dbReference type="KEGG" id="rme:Rmet_4167"/>
    <dbReference type="eggNOG" id="COG2854">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_094502_3_1_4"/>
    <dbReference type="OMA" id="MMGAWLI"/>
    <dbReference type="OrthoDB" id="1716096at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008869">
        <property type="entry name" value="MlaC/ttg2D"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36573">
        <property type="entry name" value="PTHR36573"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05494">
        <property type="entry name" value="MlaC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004649">
        <property type="entry name" value="MlaC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193415" evidence="1">
        <location>
            <begin position="27"/>
            <end position="209"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF11034.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="209" mass="23019" checksum="B8F1C83B30ACF14C" modified="2006-05-30" version="1">MNRYGFLPAAIIAPIAAVAVVSTAHAQVNTSASPDKLVQSAVEGVITKIKADPETRAGDLGKITAVVQREFLPYTDFGRTTRLAVGGAWKQATPEQQKVLQEQFTALLVRSYAVSLSQLREQSPKFSYKPAKTGSNANDVVVATRVMNNGDEMLIDYRLERGAEGWRIYDINMMGAWLIEVYRKQFADIVARDGVDGLVKYLTNHNART</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LFP9</accession>
    <name>Q1LFP9_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">pelF</name>
        <name evidence="3" type="ordered locus">Rmet_4160</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11027.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518649.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4160"/>
    <dbReference type="CAZy" id="GT4">
        <property type="family name" value="Glycosyltransferase Family 4"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF11027">
        <property type="protein sequence ID" value="ABF11027"/>
        <property type="gene ID" value="Rmet_4160"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4160"/>
    <dbReference type="eggNOG" id="COG0438">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009583_32_1_4"/>
    <dbReference type="OMA" id="VSSWVNQ"/>
    <dbReference type="OrthoDB" id="635734at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022622">
        <property type="entry name" value="DUF3492"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001296">
        <property type="entry name" value="Glyco_trans_1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11997">
        <property type="entry name" value="DUF3492"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00534">
        <property type="entry name" value="Glycos_transf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF3492" evidence="2">
        <location>
            <begin position="11"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="domain" description="Glycos_transf_1" evidence="1">
        <location>
            <begin position="314"/>
            <end position="469"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF11997"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF11027.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="529" mass="58754" checksum="046896E96B0D3AD2" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LG78</accession>
    <name>Q1LG78_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">ABC-type transport system, permease component</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">livH4</name>
        <name evidence="7" type="ordered locus">Rmet_3980</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="8">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="8">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10848.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518488.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3980"/>
    <dbReference type="EnsemblBacteria" id="ABF10848">
        <property type="protein sequence ID" value="ABF10848"/>
        <property type="gene ID" value="Rmet_3980"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3980"/>
    <dbReference type="eggNOG" id="COG0559">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039929_1_1_4"/>
    <dbReference type="OMA" id="HGAFYMA"/>
    <dbReference type="OrthoDB" id="1529037at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001851">
        <property type="entry name" value="ABC_transp_permease"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02653">
        <property type="entry name" value="BPD_transp_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0614">Plasmid</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="37"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="61"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="96"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="137"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="189"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="225"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="264"/>
            <end position="284"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABF10848.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="287" mass="30560" checksum="42086DAFF9950F31" modified="2006-05-30" version="1">MALFFQQFLNGLTLGGVYSLVALGLTLVYGILHVPNFAHGAFYMAGAYVSYYAMTTLGMNYWLAMLAAAVVVAMLSMLADRLVFYPLRHAPEIHDMIAAIGILLFLEAGAQALWGADFHRMPTPYGQMVEIFGLTAPLQRLLIIVAAFGLMVLLHLFLTRTVTGSTIVAMAQNREGAALVGIDAMRVTLLVFAISGALAAIAATLYAPINLVYPSMGNLVITKAFVIIILGGMGSIPGAIVGGLIIGMAESFGGFYISTDYKDIIAFLLLVIILSVRPQGLFAGKTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LGX0</accession>
    <name>Q1LGX0_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 6">Flagellar basal body rod protein FlgB</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">flgB</name>
        <name evidence="9" type="ordered locus">Rmet_3734</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="10">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="10">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5 6">Structural component of flagellum, the bacterial motility apparatus. Part of the rod structure of flagellar basal body.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">The basal body constitutes a major portion of the flagellar organelle and consists of a number of rings mounted on a central rod.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Bacterial flagellum basal body</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the flagella basal body rod proteins family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10606.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3734"/>
    <dbReference type="EnsemblBacteria" id="ABF10606">
        <property type="protein sequence ID" value="ABF10606"/>
        <property type="gene ID" value="Rmet_3734"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3734"/>
    <dbReference type="eggNOG" id="COG1815">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_125463_1_0_4"/>
    <dbReference type="OMA" id="EQHYEIS"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030694">
        <property type="term" value="C:bacterial-type flagellum basal body, rod"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071973">
        <property type="term" value="P:bacterial-type flagellum-dependent cell motility"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001444">
        <property type="entry name" value="Flag_bb_rod_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006300">
        <property type="entry name" value="FlgB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30435:SF12">
        <property type="entry name" value="PTHR30435:SF12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00460">
        <property type="entry name" value="Flg_bb_rod"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002889">
        <property type="entry name" value="Rod_FlgB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01396">
        <property type="entry name" value="FlgB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0975">Bacterial flagellum</keyword>
    <keyword evidence="9" id="KW-0966">Cell projection</keyword>
    <keyword evidence="9" id="KW-0969">Cilium</keyword>
    <keyword evidence="9" id="KW-0282">Flagellum</keyword>
    <keyword evidence="9" id="KW-0614">Plasmid</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Flg_bb_rod" evidence="8">
        <location>
            <begin position="8"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="51"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="51"/>
            <end position="74"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00014376"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023143"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00024934"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR002889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ABF10606.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="134" mass="14635" checksum="B157CA845F02DEE3" modified="2006-05-30" version="1">MDKLDAAFRFQQDALSLRTQRQSVIASNIAHADTPGYKARDFDFASTLAQSVERGQRSEGTSLSTTSVRHLPGQAPTSTGHELDYRIPLQSSIDGNTVEMDTERVAFADNTVHYEAGLTVMSSKIKTMLAAIQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LGY8</accession>
    <name>Q1LGY8_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_3716</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10588.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518235.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3716"/>
    <dbReference type="EnsemblBacteria" id="ABF10588">
        <property type="protein sequence ID" value="ABF10588"/>
        <property type="gene ID" value="Rmet_3716"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3716"/>
    <dbReference type="eggNOG" id="COG0716">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_068890_3_0_4"/>
    <dbReference type="OMA" id="AYYSRSG"/>
    <dbReference type="OrthoDB" id="796211at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001226">
        <property type="entry name" value="Flavodoxin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029039">
        <property type="entry name" value="Flavoprotein-like_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52218">
        <property type="entry name" value="SSF52218"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00201">
        <property type="entry name" value="FLAVODOXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF10588.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="176" mass="19238" checksum="E49453B908BDBCF5" modified="2006-05-30" version="1">MAENLVIFYSRTGTTRQAAEMLGSRLGWRVVELRDAVSRAGWWGDLRCVLDNRLGRKVGYRYDGPPLDTCGSVVVMAPVWVEHLAAPMRSFLQDQSSYKGPLAAIAVMAARGGFRAAEEVATAMGRPPHPAMVLLQQQVTTGEAKADLDEFARILQRDPRAGDAAPRSAWLSPNEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="64" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LHD2</accession>
    <name>Q1LHD2_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">17 kDa surface antigen</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_3572</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10444.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008642753.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3572"/>
    <dbReference type="EnsemblBacteria" id="ABF10444">
        <property type="protein sequence ID" value="ABF10444"/>
        <property type="gene ID" value="Rmet_3572"/>
    </dbReference>
    <dbReference type="GeneID" id="60822677"/>
    <dbReference type="KEGG" id="rme:Rmet_3572"/>
    <dbReference type="eggNOG" id="ENOG502ZKYC">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_099326_0_0_4"/>
    <dbReference type="OMA" id="AYGHRKH"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193126" evidence="2">
        <location>
            <begin position="24"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="117"/>
            <end position="147"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF10444.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="147" mass="13824" checksum="E54452790967337D" modified="2006-05-30" version="1">MSRPLRNLTLALALSAAALPVFAGGDLNNALGGGLGGVAGAAVGGALGGSTGAVIGGAVGGGAGGAITSNRRERTGAIIGGALGGGAGTAAGNAMGGRTGGLVGAGLGGAAGAALGGNVSRSNSYDDDRRYNRGYKRSKHRHHHHDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LHH1</accession>
    <name>Q1LHH1_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Phenylalanine-4-hydroxylase</fullName>
            <ecNumber evidence="5">1.14.16.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Phe-4-monooxygenase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="primary">phhA</name>
        <name evidence="14" type="ordered locus">Rmet_3533</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(6R)-L-erythro-5,6,7,8-tetrahydrobiopterin + L-phenylalanine + O2 = (4aS,6R)-4a-hydroxy-L-erythro-5,6,7,8-tetrahydrobiopterin + L-tyrosine</text>
            <dbReference type="Rhea" id="RHEA:20273"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:15642"/>
            <dbReference type="ChEBI" id="CHEBI:58095"/>
            <dbReference type="ChEBI" id="CHEBI:58315"/>
            <dbReference type="ChEBI" id="CHEBI:59560"/>
            <dbReference type="EC" id="1.14.16.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Amino-acid degradation; L-phenylalanine degradation; acetoacetate and fumarate from L-phenylalanine: step 1/6.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the biopterin-dependent aromatic amino acid hydroxylase family.</text>
    </comment>
    <dbReference type="EC" id="1.14.16.1" evidence="5"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10405.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517952.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3533"/>
    <dbReference type="EnsemblBacteria" id="ABF10405">
        <property type="protein sequence ID" value="ABF10405"/>
        <property type="gene ID" value="Rmet_3533"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3533"/>
    <dbReference type="eggNOG" id="COG3186">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023198_1_0_4"/>
    <dbReference type="OMA" id="KQFPVAT"/>
    <dbReference type="OrthoDB" id="1492162at2"/>
    <dbReference type="UniPathway" id="UPA00139">
        <property type="reaction ID" value="UER00337"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004505">
        <property type="term" value="F:phenylalanine 4-monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006559">
        <property type="term" value="P:L-phenylalanine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd03348">
        <property type="entry name" value="pro_PheOH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.800.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001273">
        <property type="entry name" value="ArAA_hydroxylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018301">
        <property type="entry name" value="ArAA_hydroxylase_Fe/CU_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036951">
        <property type="entry name" value="ArAA_hydroxylase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036329">
        <property type="entry name" value="Aro-AA_hydroxylase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019774">
        <property type="entry name" value="Aromatic-AA_hydroxylase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005960">
        <property type="entry name" value="Phe-4-hydroxylase_mono"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11473">
        <property type="entry name" value="PTHR11473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00351">
        <property type="entry name" value="Biopterin_H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00372">
        <property type="entry name" value="FYWHYDRXLASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56534">
        <property type="entry name" value="SSF56534"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01267">
        <property type="entry name" value="Phe4hydrox_mono"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00367">
        <property type="entry name" value="BH4_AAA_HYDROXYL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51410">
        <property type="entry name" value="BH4_AAA_HYDROXYL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0408">Iron</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10 14" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="8 14" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-0585">Phenylalanine catabolism</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BH4_AAA_HYDROXYL_2" evidence="13">
        <location>
            <begin position="1"/>
            <end position="310"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001060"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005088"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009712"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00020276"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023232"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00029922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51410"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ABF10405.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="310" mass="35217" checksum="8136AFAABEC9E9DC" modified="2006-05-30" version="1">MIATAAPQEIDTAFAGTMTAKLKEQFDEGLLSGQELRPDFTIAQPVHRYTDTDHAIWRKLYDRQASMLQGRVCDEFLQGLATLGMERNRVPEFDQLNETLMRATGWQVVAVPGLVPDEVFFDHLANRRFPASWWMRKPEQLDYLQEPDCFHDVFGHVPLLINPIFADYMEAYGKGGLKAAKLGALDMLARLYWYTVEFGLIRTPAGLRIFGAGILSSQGESIYSLDSASPNRIGFDLHRIMRTRYRIDTFQKTYFVIDSFEQLFDATRPDFTALYPELRTLPTLGAGDIVEGDRVINVGTREGWADSEDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="122" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LHI0</accession>
    <name>Q1LHI0_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="12">P-type ATPase CupA</fullName>
            <ecNumber evidence="12">3.6.3.4</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">cupA</name>
        <name evidence="12" type="ordered locus">Rmet_3524</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Cell membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 10">Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IB subfamily.</text>
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    <dbReference type="EnsemblBacteria" id="ABF10396">
        <property type="protein sequence ID" value="ABF10396"/>
        <property type="gene ID" value="Rmet_3524"/>
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        <property type="taxonomic scope" value="Bacteria"/>
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    <dbReference type="HOGENOM" id="CLU_001771_0_3_4"/>
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    <dbReference type="OrthoDB" id="237367at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
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    </dbReference>
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        <property type="project" value="InterPro"/>
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        <property type="term" value="F:ATPase-coupled cation transmembrane transporter activity"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="2"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SSF81665"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0186">Copper</keyword>
    <keyword evidence="5" id="KW-0187">Copper transport</keyword>
    <keyword evidence="12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0406">Ion transport</keyword>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-1278">Translocase</keyword>
    <keyword evidence="2 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="198"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="228"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="255"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="411"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="440"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="755"/>
            <end position="774"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
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            <end position="797"/>
        </location>
    </feature>
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        <location>
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            <end position="77"/>
        </location>
    </feature>
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        <location>
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            <end position="145"/>
        </location>
    </feature>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022796"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023008"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="RuleBase" id="RU362081"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ABF10396.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="813" mass="84187" checksum="1102502E62909EE6" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LHS7</accession>
    <name>Q1LHS7_CUPMC</name>
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        <submittedName>
            <fullName evidence="7">Cytochrome subunit of sulfide dehydrogenase, Flavocytochrome c cytochrome subunit, putative soxE-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Rmet_3427</name>
    </gene>
    <organism evidence="7 8">
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        <name type="synonym">Ralstonia metallidurans</name>
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            <taxon>Betaproteobacteria</taxon>
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            <taxon>Burkholderiaceae</taxon>
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        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
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    </dbReference>
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    </dbReference>
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        <property type="component" value="Chromosome"/>
    </dbReference>
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    </dbReference>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009056">
        <property type="entry name" value="Cyt_c-like_dom"/>
    </dbReference>
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        <property type="entry name" value="Cyt_c-like_dom_sf"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
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        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
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        <location>
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        </location>
    </feature>
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        <location>
            <begin position="32"/>
            <end position="125"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABF10299.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="125" mass="12967" checksum="6D03B2028CA559D4" modified="2006-05-30" version="1">MTLAPRYLRGVVAGFCVAPSLALAQPASAPAGPAEAGKLTVEAQAARTMQARNLAASCAVCHGTDGKPPADGPIPRLAGRQQADLVELMFNFKNGKRSGTVMPQIAKGYSDAQILAMAAWFADQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LIK5</accession>
    <name>Q1LIK5_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Transcriptional regulator, TetR-family</fullName>
        </submittedName>
    </protein>
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        <name evidence="7" type="ordered locus">Rmet_3149</name>
    </gene>
    <organism evidence="7 8">
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        <name type="synonym">Ralstonia metallidurans</name>
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        <lineage>
            <taxon>Bacteria</taxon>
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        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10021.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3149"/>
    <dbReference type="EnsemblBacteria" id="ABF10021">
        <property type="protein sequence ID" value="ABF10021"/>
        <property type="gene ID" value="Rmet_3149"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3149"/>
    <dbReference type="eggNOG" id="COG1309">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069356_12_4_4"/>
    <dbReference type="OMA" id="PHIAERQ"/>
    <dbReference type="OrthoDB" id="1437290at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023772">
        <property type="entry name" value="DNA-bd_HTH_TetR-type_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001647">
        <property type="entry name" value="HTH_TetR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041490">
        <property type="entry name" value="KstR2_TetR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036271">
        <property type="entry name" value="Tet_transcr_reg_TetR-rel_C_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17932">
        <property type="entry name" value="TetR_C_24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00440">
        <property type="entry name" value="TetR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00455">
        <property type="entry name" value="HTHTETR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48498">
        <property type="entry name" value="SSF48498"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01081">
        <property type="entry name" value="HTH_TETR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50977">
        <property type="entry name" value="HTH_TETR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0678">Repressor</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH tetR-type" evidence="6">
        <location>
            <begin position="47"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="H-T-H motif" evidence="5">
        <location>
            <begin position="70"/>
            <end position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABF10021.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="233" mass="26418" checksum="D62A42FE08D19B4D" modified="2006-05-30" version="1">MVVRTGRARMADMPRALKRTGHTPRAAEATTLAESGPARAHVAERQEARRRQILDAAGQLFYTKGYPGMTMNDLCRALGVTKPAVYYYFADKYEIFDILCRESAQTCLTVIRETADPALPVRERLSACLLEMARRCVRCHVAAALSFRDNQYLRPDTVEWLNTMARDFYRDLYALLEEGREAGVFEFGDARVTAHSMGGVIGFMYTWHEPGRMDPETLAQELARNMMKLVLPN</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="64" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LIW0</accession>
    <name>Q1LIW0_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_3044</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09916.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_003056224.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3044"/>
    <dbReference type="EnsemblBacteria" id="ABF09916">
        <property type="protein sequence ID" value="ABF09916"/>
        <property type="gene ID" value="Rmet_3044"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3044"/>
    <dbReference type="eggNOG" id="COG4804">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046640_1_1_4"/>
    <dbReference type="OMA" id="LTHQDVG"/>
    <dbReference type="OrthoDB" id="466535at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1350.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011856">
        <property type="entry name" value="tRNA_endonuc-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009362">
        <property type="entry name" value="YhcG_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041527">
        <property type="entry name" value="YhcG_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17761">
        <property type="entry name" value="DUF1016_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06250">
        <property type="entry name" value="YhcG_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF1016_N" evidence="2">
        <location>
            <begin position="27"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="domain" description="YhcG_C" evidence="1">
        <location>
            <begin position="206"/>
            <end position="358"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF06250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF17761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF09916.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="378" mass="42768" checksum="DABBAAB6DD4C27FC" modified="2006-05-30" version="1">MTKKAPAMAGVAPAAVPAGYAGIHGGIVDLLDAARQTAARSVNALMTASYWEIGRRIVEAEQQGKRRAGYGEQLIERLASDLTQQFGRGFSRQNLQQMRSFFLTRPIRQTVSGESSPEPVRPWRLDELAQVFTLPWSAYVRLLSVKDEHARQFYEAEALRGGWSVRQLDRQIGSQFYERTALSKNKAAMLAKGAVAKPEDAVAPGDAIKDPYVLEFLDLKDEYSESDLEAALIRRLEDFLLELGEGFTFIGRQRRLRIDQTWYRVDLLLFHRKLRCLVIIDLKLGSLTHADVGQMHMYCNYAKEHWAYPEENPPVGLILCADKGLALARYALEGLPTKVMAANYRMVLPDTDVLQKELESTRLLLESRGTIRPKTWKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="55" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LIW6</accession>
    <name>Q1LIW6_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_3038</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09910.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_003056217.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3038"/>
    <dbReference type="EnsemblBacteria" id="ABF09910">
        <property type="protein sequence ID" value="ABF09910"/>
        <property type="gene ID" value="Rmet_3038"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3038"/>
    <dbReference type="PATRIC" id="fig|402626.5.peg.3740"/>
    <dbReference type="eggNOG" id="COG3616">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_119528_1_1_4"/>
    <dbReference type="OMA" id="TQFEDES"/>
    <dbReference type="OrthoDB" id="1558145at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021341">
        <property type="entry name" value="DUF2958"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11171">
        <property type="entry name" value="DUF2958"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF09910.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="116" mass="12847" checksum="B85B1CD67E178F9A" modified="2006-05-30" version="1">MNHAFITDEQRIVLLANGRESLENPDFDPAPVVKLFTPDAGATWLLTEIDPDDHDHAFGLCDLGLGMPEIGWVSLSELATVRGRLGLPVERDLHFRAEKRLSAYARDARLAGRVVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LJ44</accession>
    <name>Q1LJ44_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 4">Phosphatase NudJ</fullName>
            <ecNumber evidence="4">3.6.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">nudJ</name>
        <name evidence="6" type="ordered locus">Rmet_2959</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="4">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="2 4">Monomer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the Nudix hydrolase family. NudJ subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.1.-" evidence="4"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09832.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008641895.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2959"/>
    <dbReference type="EnsemblBacteria" id="ABF09832">
        <property type="protein sequence ID" value="ABF09832"/>
        <property type="gene ID" value="Rmet_2959"/>
    </dbReference>
    <dbReference type="GeneID" id="60820604"/>
    <dbReference type="KEGG" id="rme:Rmet_2959"/>
    <dbReference type="eggNOG" id="COG1051">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037162_6_0_4"/>
    <dbReference type="OMA" id="QPAGHLD"/>
    <dbReference type="OrthoDB" id="1846332at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017110">
        <property type="term" value="F:nucleoside-diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017111">
        <property type="term" value="F:nucleoside-triphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004787">
        <property type="term" value="F:thiamine-diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03675">
        <property type="entry name" value="Nudix_Hydrolase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015797">
        <property type="entry name" value="NUDIX_hydrolase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000086">
        <property type="entry name" value="NUDIX_hydrolase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033713">
        <property type="entry name" value="NudJ"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00293">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55811">
        <property type="entry name" value="SSF55811"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51462">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nudix hydrolase" evidence="5">
        <location>
            <begin position="7"/>
            <end position="140"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007608"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00015552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU364043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABF09832.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="183" mass="19712" checksum="F6010596227F4901" modified="2006-05-30" version="1">MCTMSRDWNASVTVAAVIERGGRFLLVEEETADGLRLNQPAGHLEAGESLIRAVVRETLEETAHSFEPRALLGVYTSRGRSSRDQGDTTYIRFAFTGDIGTLDAGRQLDTGIVRTVWMTAEELRGCPERHRSPLLMACVDDYLAGKRFALDMIHTHPSTVAASIAPGDTAAPTNPPAQAGAAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LJ47</accession>
    <name>Q1LJ47_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 11">Adenylosuccinate lyase</fullName>
            <shortName evidence="11">ASL</shortName>
            <ecNumber evidence="4 11">4.3.2.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10 11">Adenylosuccinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="primary">purB</name>
        <name evidence="14" type="ordered locus">Rmet_2956</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Catalyzes two reactions in de novo purine nucleotide biosynthesis. Catalyzes the breakdown of 5-aminoimidazole- (N-succinylocarboxamide) ribotide (SAICAR or 2-[5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamido]succinate) to 5-aminoimidazole-4-carboxamide ribotide (AICAR or 5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide) and fumarate, and of adenylosuccinate (ADS or N(6)-(1,2-dicarboxyethyl)-AMP) to adenosine monophosphate (AMP) and fumarate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>(2S)-2-[5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamido]succinate = 5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + fumarate</text>
            <dbReference type="Rhea" id="RHEA:23920"/>
            <dbReference type="ChEBI" id="CHEBI:29806"/>
            <dbReference type="ChEBI" id="CHEBI:58443"/>
            <dbReference type="ChEBI" id="CHEBI:58475"/>
            <dbReference type="EC" id="4.3.2.2"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="7">
            <dbReference type="Rhea" id="RHEA:23921"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>N(6)-(1,2-dicarboxyethyl)-AMP = AMP + fumarate</text>
            <dbReference type="Rhea" id="RHEA:16853"/>
            <dbReference type="ChEBI" id="CHEBI:29806"/>
            <dbReference type="ChEBI" id="CHEBI:57567"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="4.3.2.2"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="8">
            <dbReference type="Rhea" id="RHEA:16854"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 11">Purine metabolism; AMP biosynthesis via de novo pathway; AMP from IMP: step 2/2.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1 11">Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide from 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate: step 2/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the lyase 1 family. Adenylosuccinate lyase subfamily.</text>
    </comment>
    <dbReference type="EC" id="4.3.2.2" evidence="4 11"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09829.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008641901.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2956"/>
    <dbReference type="EnsemblBacteria" id="ABF09829">
        <property type="protein sequence ID" value="ABF09829"/>
        <property type="gene ID" value="Rmet_2956"/>
    </dbReference>
    <dbReference type="GeneID" id="60820607"/>
    <dbReference type="KEGG" id="rme:Rmet_2956"/>
    <dbReference type="eggNOG" id="COG0015">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025566_2_0_4"/>
    <dbReference type="OMA" id="TQVNPCD"/>
    <dbReference type="UniPathway" id="UPA00074">
        <property type="reaction ID" value="UER00132"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00075">
        <property type="reaction ID" value="UER00336"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070626">
        <property type="term" value="F:(S)-2-(5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamido) succinate lyase (fumarate-forming) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004018">
        <property type="term" value="F:N6-(1,2-dicarboxyethyl)AMP AMP-lyase (fumarate-forming) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044208">
        <property type="term" value="P:'de novo' AMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.275.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024083">
        <property type="entry name" value="Fumarase/histidase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020557">
        <property type="entry name" value="Fumarate_lyase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000362">
        <property type="entry name" value="Fumarate_lyase_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022761">
        <property type="entry name" value="Fumarate_lyase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008948">
        <property type="entry name" value="L-Aspartase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004769">
        <property type="entry name" value="Pur_lyase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013539">
        <property type="entry name" value="PurB_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08328">
        <property type="entry name" value="ASL_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00206">
        <property type="entry name" value="Lyase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00149">
        <property type="entry name" value="FUMRATELYASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48557">
        <property type="entry name" value="SSF48557"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00928">
        <property type="entry name" value="purB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00163">
        <property type="entry name" value="FUMARATE_LYASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 14" id="KW-0456">Lyase</keyword>
    <keyword evidence="6 11" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Lyase_1" evidence="12">
        <location>
            <begin position="17"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="domain" description="ASL_C" evidence="13">
        <location>
            <begin position="334"/>
            <end position="448"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00017058"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00024477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00024487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00025012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00030717"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU361172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF08328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ABF09829.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="458" mass="50579" checksum="47198A90710DB472" modified="2006-05-30" version="1">MSTSSLSPLTALSPIDGRYAAKADALREWLSEAAFMRNRVKVEVNWLIALAQAGLPDVPKFSAASEAKLLSLVENFTEADAARIKEIEAVTNHDVKAVEYWLKEQVKGNAELEAASEFIHFACTSEDINNTSHGMMLKGARDGVIVPALKRVHARLVELAKLNAAQPMLSRTHGQPASPTTLGKEMANVAARLARAIERVERVELLGKMNGAVGNYNAHLSAYPTFDWESFSKQVIETRLGLTFNPYTIQIEPHDYMAELFDAVARANTILLDLNRDVWGYISVGYFKQKTKAGEIGSSTMPHKVNPIDFENSEGNVGLANAVLRHLSEKLPVSRWQRDLTDSTVLRNMGVAFGYSLLAYEACLRGLGKLETNPERLNEDLDNCWEVLAEPVQTVMRRFGVPNPYEQLKELTRGKGISREALQTFVQGLAIPDDAKALLLAMTPASYIGRAVELANRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LJ88</accession>
    <name>Q1LJ88_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">2-amino-4-hydroxy-6-hydroxymethyldihydropteridine pyrophosphokinase</fullName>
            <ecNumber evidence="3">2.7.6.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">7,8-dihydro-6-hydroxymethylpterin-pyrophosphokinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="primary">folK</name>
        <name evidence="14" type="ordered locus">Rmet_2915</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Catalyzes the transfer of pyrophosphate from adenosine triphosphate (ATP) to 6-hydroxymethyl-7,8-dihydropterin, an enzymatic step in folate biosynthesis pathway.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; tetrahydrofolate biosynthesis; 2-amino-4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine diphosphate from 7,8-dihydroneopterin triphosphate: step 4/4.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the HPPK family.</text>
    </comment>
    <dbReference type="EC" id="2.7.6.3" evidence="3"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09788.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517461.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2915"/>
    <dbReference type="EnsemblBacteria" id="ABF09788">
        <property type="protein sequence ID" value="ABF09788"/>
        <property type="gene ID" value="Rmet_2915"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2915"/>
    <dbReference type="eggNOG" id="COG0801">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_097916_2_0_4"/>
    <dbReference type="OMA" id="QVILRQN"/>
    <dbReference type="OrthoDB" id="1953248at2"/>
    <dbReference type="UniPathway" id="UPA00077">
        <property type="reaction ID" value="UER00155"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003848">
        <property type="term" value="F:2-amino-4-hydroxy-6-hydroxymethyldihydropteridine diphosphokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046656">
        <property type="term" value="P:folic acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046654">
        <property type="term" value="P:tetrahydrofolate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00483">
        <property type="entry name" value="HPPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.560">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000550">
        <property type="entry name" value="Hppk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035907">
        <property type="entry name" value="Hppk_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01288">
        <property type="entry name" value="HPPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55083">
        <property type="entry name" value="SSF55083"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01498">
        <property type="entry name" value="folK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00794">
        <property type="entry name" value="HPPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-0289">Folate biosynthesis</keyword>
    <keyword evidence="7 14" id="KW-0418">Kinase</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 14" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="HPPK" evidence="13">
        <location>
            <begin position="87"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005051"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005810"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013253"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00016218"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00029409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00029766"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00033413"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS00794"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ABF09788.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="173" mass="18790" checksum="78900844CCF29C75" modified="2006-05-30" version="1">MTLAFIGIGANLGDARQAVKDAIVCLAQQVGITVVAKSSFYRTAPVDATGADYFNAVVKIETPFTAAQLLRICHHIEDQFGRERPFRNAPRTLDLDLLLFGAESINTEALTVPHPRITERAFVLVPLVELDPAIEIPGVGPAAGFLPDVQEQRIEKFSSCKCSRMKEGDGARP</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LJI3</accession>
    <name>Q1LJI3_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">ABC-type transporter, periplasmic component: HAAT family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_2820</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09693.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517386.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2820"/>
    <dbReference type="EnsemblBacteria" id="ABF09693">
        <property type="protein sequence ID" value="ABF09693"/>
        <property type="gene ID" value="Rmet_2820"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2820"/>
    <dbReference type="eggNOG" id="COG0683">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027128_0_1_4"/>
    <dbReference type="OMA" id="AYGESWW"/>
    <dbReference type="OrthoDB" id="776281at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028081">
        <property type="entry name" value="Leu-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028082">
        <property type="entry name" value="Peripla_BP_I"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13458">
        <property type="entry name" value="Peripla_BP_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53822">
        <property type="entry name" value="SSF53822"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193554" evidence="1">
        <location>
            <begin position="26"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="domain" description="Peripla_BP_6" evidence="2">
        <location>
            <begin position="27"/>
            <end position="381"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF09693.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="389" mass="41830" checksum="FF61241CEB0D8682" modified="2006-05-30" version="1">MRLRKLLLPVMSMLSFGVATGSAHADIRVGVDLSTTGPAAAIGIPSRNTVQMWPQTLGGQRAQYIVLDDATDPAAAVRNVRKLIAEDKVDVIVGPNITPNAIAALDAIAESGTPMVALAASASIIEPAGDARRRWAFKMPQNDALMATILTEHMTNQGVKTVGFIGFNDAYGESWWREFSKMAEIRRLRVVGNERFGRTDTSVTGQILKLMSANPDAILIAGSGTPAVLPQKTLGERGYKGRIYQTHGIASWDFLRVGGKDVEGTLFPTGPVVVARQLPDSHPVKKVALDFVTRYEAKYGPDTVTQFAGDAWGAWLLLDDASRRALKSGAQPGTPAFRAALRDAIESTHNLTVPNGVLNLSAQDHQGFDQRARVMGVVRNGKFAYAGDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="104" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LJJ9</accession>
    <name>Q1LJJ9_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="14 15">Dihydroxy-acid dehydratase</fullName>
            <shortName evidence="15">DAD</shortName>
            <ecNumber evidence="14 15">4.2.1.9</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15 16" type="primary">ilvD</name>
        <name evidence="16" type="ordered locus">Rmet_2804</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="15">Functions in the biosynthesis of branched-chain amino acids. Catalyzes the dehydration of (2R,3R)-2,3-dihydroxy-3-methylpentanoate (2,3-dihydroxy-3-methylvalerate) into 2-oxo-3-methylpentanoate (2-oxo-3-methylvalerate) and of (2R)-2,3-dihydroxy-3-methylbutanoate (2,3-dihydroxyisovalerate) into 2-oxo-3-methylbutanoate (2-oxoisovalerate), the penultimate precursor to L-isoleucine and L-valine, respectively.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>(2R)-2,3-dihydroxy-3-methylbutanoate = 3-methyl-2-oxobutanoate + H2O</text>
            <dbReference type="Rhea" id="RHEA:24809"/>
            <dbReference type="ChEBI" id="CHEBI:11851"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:49072"/>
            <dbReference type="EC" id="4.2.1.9"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:24810"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>(2R,3R)-2,3-dihydroxy-3-methylpentanoate = (S)-3-methyl-2-oxopentanoate + H2O</text>
            <dbReference type="Rhea" id="RHEA:27694"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:35146"/>
            <dbReference type="ChEBI" id="CHEBI:49258"/>
            <dbReference type="EC" id="4.2.1.9"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="15">
            <name>2Fe-2S cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49601"/>
        </cofactor>
        <text evidence="15">Binds 1 [2Fe-2S] cluster per subunit. This cluster acts as a Lewis acid cofactor.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 15">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="13 15">Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 3/4.</text>
    </comment>
    <comment type="pathway">
        <text evidence="12 15">Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 3/4.</text>
    </comment>
    <comment type="subunit">
        <text evidence="15">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 15">Belongs to the IlvD/Edd family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.9" evidence="14 15"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09677.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517373.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2804"/>
    <dbReference type="EnsemblBacteria" id="ABF09677">
        <property type="protein sequence ID" value="ABF09677"/>
        <property type="gene ID" value="Rmet_2804"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2804"/>
    <dbReference type="eggNOG" id="COG0129">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014271_4_2_4"/>
    <dbReference type="OMA" id="PFGRYVM"/>
    <dbReference type="OrthoDB" id="193579at2"/>
    <dbReference type="UniPathway" id="UPA00047">
        <property type="reaction ID" value="UER00057"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00049">
        <property type="reaction ID" value="UER00061"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004160">
        <property type="term" value="F:dihydroxy-acid dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009097">
        <property type="term" value="P:isoleucine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009099">
        <property type="term" value="P:valine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.30.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00012">
        <property type="entry name" value="IlvD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042096">
        <property type="entry name" value="Dihydro-acid_dehy_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004404">
        <property type="entry name" value="DihydroxyA_deHydtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000581">
        <property type="entry name" value="DiOHA_6PGluconate_deHydtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020558">
        <property type="entry name" value="DiOHA_6PGluconate_deHydtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037237">
        <property type="entry name" value="IlvD/EDD_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00920">
        <property type="entry name" value="ILVD_EDD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143975">
        <property type="entry name" value="SSF143975"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00110">
        <property type="entry name" value="ilvD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00886">
        <property type="entry name" value="ILVD_EDD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00887">
        <property type="entry name" value="ILVD_EDD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 15" id="KW-0001">2Fe-2S</keyword>
    <keyword evidence="3 15" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="10 15" id="KW-0100">Branched-chain amino acid biosynthesis</keyword>
    <keyword evidence="7 15" id="KW-0408">Iron</keyword>
    <keyword evidence="8 15" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="9 15 16" id="KW-0456">Lyase</keyword>
    <keyword evidence="6 15" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5 15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Proton acceptor" evidence="15">
        <location>
            <position position="473"/>
        </location>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="50"/>
        </location>
        <ligand>
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="82"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="124"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" description="via carbamate group" evidence="15">
        <location>
            <position position="125"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="447"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="modified residue" description="N6-carboxylysine" evidence="15">
        <location>
            <position position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006486"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00029304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00029436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00029437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00029490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="ABF09677.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="557" mass="59181" checksum="DFC36B4017C66EC0" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LJP5</accession>
    <name>Q1LJP5_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Formate dehydrogenase subunit C</fullName>
            <ecNumber evidence="12">1.2.1.2</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">fdhC</name>
        <name evidence="12" type="ordered locus">Rmet_2758</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the formate dehydrogenase gamma subunit family.</text>
    </comment>
    <dbReference type="EC" id="1.2.1.2" evidence="12"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09631.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517330.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2758"/>
    <dbReference type="EnsemblBacteria" id="ABF09631">
        <property type="protein sequence ID" value="ABF09631"/>
        <property type="gene ID" value="Rmet_2758"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2758"/>
    <dbReference type="eggNOG" id="COG2864">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047957_2_1_4"/>
    <dbReference type="OMA" id="HIYMGTI"/>
    <dbReference type="OrthoDB" id="1612137at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009326">
        <property type="term" value="C:formate dehydrogenase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008863">
        <property type="term" value="F:formate dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022904">
        <property type="term" value="P:respiratory electron transport chain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011577">
        <property type="entry name" value="Cyt_b561_bac/Ni-Hgenase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016174">
        <property type="entry name" value="Di-haem_cyt_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006471">
        <property type="entry name" value="Formate_DH_gsu"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01292">
        <property type="entry name" value="Ni_hydr_CYTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81342">
        <property type="entry name" value="SSF81342"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01583">
        <property type="entry name" value="formate-DH-gamm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0732">Signal</keyword>
    <keyword evidence="5 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193557" evidence="10">
        <location>
            <begin position="26"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="137"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="179"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="224"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="280"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="320"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="domain" description="Ni_hydr_CYTB" evidence="11">
        <location>
            <begin position="172"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="31"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="76"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="374"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="31"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="81"/>
            <end position="109"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF01292"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ABF09631.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="397" mass="43783" checksum="B72EA923958626A4" modified="2006-05-30" version="1">MTRLSRTGRWLAGALMTLAMAGAFAQAPASAPQAQQSATQSATQSDNANNPATHPAQPFAGIASENIFNVPRRDMAAEARAQQERSQTQPGNNAPVWREANSDQPNYSSLPAKEAGVLIQRTGQKWRLFRNGVITVWGGWLVLIVPLAILAFFAWRGTIPLKTPRTGRMIERFTPLERIVHWTMAISFSILAISGLVILFGKHVLLPIFGHTLFGWLTYILKNLHNLSGPVFTVAIVVAFVLFVRDNLPSRDDLTWIARAGGLLTGSHVPSHRFNAGEKLWFWVGLVFFGLILSASGWVLDMIVPGLEYYRATMQIANVIHAIAAVLMIALACGHIYMGTIGMEGAYRAMRDGYVDEAWAQEHHEHWYDDIKAGKIPAQRSTPPEDAGRSRQRPGQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LJT9</accession>
    <name>Q1LJT9_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 9">3-deoxy-D-manno-octulosonic acid transferase</fullName>
            <shortName evidence="9">Kdo transferase</shortName>
            <ecNumber evidence="3 9">2.4.99.12</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6 9">Lipid IV(A) 3-deoxy-D-manno-octulosonic acid transferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">kdtA</name>
        <name evidence="11" type="ordered locus">Rmet_2714</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Involved in lipopolysaccharide (LPS) biosynthesis. Catalyzes the transfer of 3-deoxy-D-manno-octulosonate (Kdo) residue(s) from CMP-Kdo to lipid IV(A), the tetraacyldisaccharide-1,4'-bisphosphate precursor of lipid A.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 9">
            <text>CMP-3-deoxy-beta-D-manno-octulosonate + lipid IVA (E. coli) = alpha-Kdo-(2-&gt;6)-lipid IVA + CMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:28066"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:58603"/>
            <dbReference type="ChEBI" id="CHEBI:60364"/>
            <dbReference type="ChEBI" id="CHEBI:60377"/>
            <dbReference type="ChEBI" id="CHEBI:85987"/>
            <dbReference type="EC" id="2.4.99.12"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 9">Bacterial outer membrane biogenesis; LPS core biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cell inner membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the glycosyltransferase group 1 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.99.12" evidence="3 9"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09587.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517286.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2714"/>
    <dbReference type="CAZy" id="GT30">
        <property type="family name" value="Glycosyltransferase Family 30"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF09587">
        <property type="protein sequence ID" value="ABF09587"/>
        <property type="gene ID" value="Rmet_2714"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2714"/>
    <dbReference type="eggNOG" id="COG1519">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036146_2_0_4"/>
    <dbReference type="OMA" id="FIKYEFW"/>
    <dbReference type="OrthoDB" id="1163086at2"/>
    <dbReference type="UniPathway" id="UPA00958"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043842">
        <property type="term" value="F:Kdo transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009244">
        <property type="term" value="P:lipopolysaccharide core region biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11720">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007507">
        <property type="entry name" value="Glycos_transf_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038107">
        <property type="entry name" value="Glycos_transf_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039901">
        <property type="entry name" value="Kdotransferase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42755">
        <property type="entry name" value="PTHR42755"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04413">
        <property type="entry name" value="Glycos_transf_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="9" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-0448">Lipopolysaccharide biosynthesis</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 9 11" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glycos_transf_N" evidence="10">
        <location>
            <begin position="34"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="7">
        <location>
            <position position="61"/>
        </location>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="8">
        <location>
            <position position="133"/>
        </location>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="8">
        <location>
            <position position="209"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000183"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004713"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012621"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00019077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00031445"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR639901-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR639901-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU365103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF04413"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ABF09587.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="424" mass="46321" checksum="04640AA9FB5A4A2D" modified="2006-05-30" version="1">MLRFVYSLLWLLILPLALLRLVWRSRKESGYIQHVGERLGRYGDLSAAGPWLWVHAVSVGETRAAQPLIDALLAAYPRHRLLLTHMTPTGRQTGAQLYGANPRVQQCYLPYDLPWLVRRFLAYFQPDAGLIMETEVWPNLVHVTRRMGVPLFLVNARLSPRSYRRTARFGGAAAALYNDFTMVLAQTAGDAERYRALGVKSVQVTGNLKFDMQPPEAGVARGQLLRQAFGNRHVLAAASTREGEEPLILEAFSRWPGTDRPALLLVPRHPQRFDEVAAMATRAGFSVQRRSALDIEQLSAPITADVVLGDSMGEMAMYYAASDIAYIGGSLIPLGGQNLIEACAVGTPVLMGPHTFNFAQATEDAIAAGACQRVADADELMVAAAAILGDATRLADMREHALTFAGLHRGATARTLGALAPVLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LJU1</accession>
    <name>Q1LJU1_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Putative cell surface polysaccharide polymerase/ligase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Rmet_2712</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <reference evidence="6 7" key="2">
        <citation type="journal article" date="2022" name="Nature" volume="0" first="0" last="0">
            <title>Structural basis of lipopolysaccharide maturation by the O-antigen ligase.</title>
            <authorList>
                <person name="Ashraf K.U."/>
                <person name="Nygaard R."/>
                <person name="Vickery O.N."/>
                <person name="Erramilli S.K."/>
                <person name="Herrera C.M."/>
                <person name="McConville T.H."/>
                <person name="Petrou V.I."/>
                <person name="Giacometti S.I."/>
                <person name="Dufrisne M.B."/>
                <person name="Nosol K."/>
                <person name="Zinkle A.P."/>
                <person name="Graham C.L.B."/>
                <person name="Loukeris M."/>
                <person name="Kloss B."/>
                <person name="Skorupinska-Tudek K."/>
                <person name="Swiezewska E."/>
                <person name="Roper D."/>
                <person name="Clarke O.B."/>
                <person name="Uhlemann A.C."/>
                <person name="Kossiakoff A.A."/>
                <person name="Trent M.S."/>
                <person name="Stansfeld P.J."/>
                <person name="Mancia F."/>
            </authorList>
        </citation>
        <scope>STRUCTURE BY ELECTRON MICROSCOPY (3.23 ANGSTROMS)</scope>
        <scope>DISULFIDE BONDS</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09585.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517284.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="PDB" id="7TPG">
        <property type="method" value="EM"/>
        <property type="resolution" value="3.23 A"/>
        <property type="chains" value="B=1-413"/>
    </dbReference>
    <dbReference type="PDB" id="7TPJ">
        <property type="method" value="EM"/>
        <property type="resolution" value="3.46 A"/>
        <property type="chains" value="B=1-413"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2712"/>
    <dbReference type="EnsemblBacteria" id="ABF09585">
        <property type="protein sequence ID" value="ABF09585"/>
        <property type="gene ID" value="Rmet_2712"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2712"/>
    <dbReference type="eggNOG" id="COG3307">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_049451_0_1_4"/>
    <dbReference type="OMA" id="TNAIQWG"/>
    <dbReference type="OrthoDB" id="754057at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016874">
        <property type="term" value="F:ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007016">
        <property type="entry name" value="O-antigen_ligase-related"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04932">
        <property type="entry name" value="Wzy_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword evidence="6 7" id="KW-0002">3D-structure</keyword>
    <keyword evidence="4" id="KW-0436">Ligase</keyword>
    <keyword evidence="2 3" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="7"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="30"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="60"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="112"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="142"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="174"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="212"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="325"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="358"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="382"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="6 7">
        <location>
            <begin position="299"/>
            <end position="307"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF09585.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="6">
        <source>
            <dbReference type="PDB" id="7TPG"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="7">
        <source>
            <dbReference type="PDB" id="7TPJ"/>
        </source>
    </evidence>
    <sequence length="413" mass="44495" checksum="1F6AE6DC4C7B1015" modified="2006-05-30" version="1">MTDSNKLLSRMATVLVFAFPVLILCVPRGAGVFLAGVGVLALLGWRGMGRAWREYSKVMTPLAIAVLAFMLVYVGSKLYFHTPWNVIDNPSRTLLAILTCWVIVRAAPNPAWLWRGITVGLFLALLIVGYQKFALNIDRPSAWIQAIAFANMIAALALVGFARPGDSRGTHMEAWVNLLLGTMILMLNGTRGAVVAMLVTSVPMLMIRYRRFSVRMLIVAVCAVATLAIGAYMVPDSPVSKRVDDAVSEIQMYRQGNIETSVGVRLKIWHIGLQYFSEHPWTGVGVGQFARILHASEFCHETKSLACVLEHAHNDIVEAASTTGIPGLMVMLGLFLVPAVLFARALRAARSLGNPQGVSLGGAGLGVVMASLISGLTQVTMAHQANVVFYAGLIGLLLGMAGREAHSARTDAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LJW4</accession>
    <name>Q1LJW4_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Riboflavin synthase alpha chain</fullName>
            <ecNumber evidence="4">2.5.1.9</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">ribC</name>
        <name evidence="4" type="ordered locus">Rmet_2689</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.5.1.9" evidence="4"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09562.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517262.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2689"/>
    <dbReference type="EnsemblBacteria" id="ABF09562">
        <property type="protein sequence ID" value="ABF09562"/>
        <property type="gene ID" value="Rmet_2689"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2689"/>
    <dbReference type="eggNOG" id="COG0307">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_034388_2_2_4"/>
    <dbReference type="OMA" id="HFVTGHV"/>
    <dbReference type="OrthoDB" id="1276859at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004746">
        <property type="term" value="F:riboflavin synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd00402">
        <property type="entry name" value="Riboflavin_synthase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023366">
        <property type="entry name" value="ATP_synth_asu-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001783">
        <property type="entry name" value="Lumazine-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026017">
        <property type="entry name" value="Lumazine-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017938">
        <property type="entry name" value="Riboflavin_synthase-like_b-brl"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21098">
        <property type="entry name" value="PTHR21098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00677">
        <property type="entry name" value="Lum_binding"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000498">
        <property type="entry name" value="Riboflavin_syn_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63380">
        <property type="entry name" value="SSF63380"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00187">
        <property type="entry name" value="ribE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51177">
        <property type="entry name" value="LUMAZINE_BIND"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="repeat" description="Lumazine-binding" evidence="2">
        <location>
            <begin position="1"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="domain" description="Lumazine-binding" evidence="3">
        <location>
            <begin position="1"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="domain" description="Lumazine-binding" evidence="3">
        <location>
            <begin position="99"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="repeat" description="Lumazine-binding" evidence="2">
        <location>
            <begin position="99"/>
            <end position="199"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00524"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF09562.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="210" mass="22050" checksum="74988CC7EE01E91F" modified="2006-05-30" version="1">MFTGIVAAVGRIESVSPLGDVNAGVRLRVAAGDLDLSDVGIGDSIAIQGACMTVVTLGAGQFDVDVSRESLDKTVGLDQPGRVNLEKALRLADRLGGHLVSGHVDGLGEVVRFAPVGESHELRIRAPRELGRYLAYKGSVVVNGVSLTVNNVSDEADGCVFSINLIPHTVEVTTLNELAPGKRVNLEIDLIARYVERMLSASADAGQPLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="100" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LK13</accession>
    <name>Q1LK13_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Thioredoxin domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Rmet_2636</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the thioredoxin family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09513.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517212.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2636"/>
    <dbReference type="EnsemblBacteria" id="ABF09513">
        <property type="protein sequence ID" value="ABF09513"/>
        <property type="gene ID" value="Rmet_2636"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2636"/>
    <dbReference type="eggNOG" id="COG3118">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046120_1_1_4"/>
    <dbReference type="OMA" id="QPRNMSM"/>
    <dbReference type="OrthoDB" id="1630944at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015035">
        <property type="term" value="F:protein-disulfide reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005746">
        <property type="entry name" value="Thioredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017937">
        <property type="entry name" value="Thioredoxin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00085">
        <property type="entry name" value="Thioredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="SSF48452"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="SSF52833"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01068">
        <property type="entry name" value="thioredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00194">
        <property type="entry name" value="THIOREDOXIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Thioredoxin" evidence="5">
        <location>
            <begin position="1"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABF09513.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="280" mass="30743" checksum="DFE44B20D3B9AA1F" modified="2006-05-30" version="1">MSDVTLQNFEADVIEMSRQVPVLVDFWAPWCGPCRTLGPMLEKLEAESGGKWRLAKVNVDENQELAAHFGVRSIPHVVAFADGQAVDQFIGVLPESQLREFLDRLTPNPGELALREAVELAAAGDREAAQASFQAALAYDPGADTARLTYIGFLLDGNKIADAETEFGLLSPRAAQEDAYAALQTRLEAMKGVGDLPDGADLKARVAANPADLPARLDLAHVLIARREYEAALEQLLEIVRSDRWFEDDVGRKTMLSVFELMGDDPAVSRWRRQLATVLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="125" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LK19</accession>
    <name>Q1LK19_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Valine--tRNA ligase</fullName>
            <ecNumber evidence="8">6.1.1.9</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Valyl-tRNA synthetase</fullName>
            <shortName evidence="8">ValRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8 13" type="primary">valS</name>
        <name evidence="13" type="ordered locus">Rmet_2630</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Catalyzes the attachment of valine to tRNA(Val). As ValRS can inadvertently accommodate and process structurally similar amino acids such as threonine, to avoid such errors, it has a 'posttransfer' editing activity that hydrolyzes mischarged Thr-tRNA(Val) in a tRNA-dependent manner.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 8">
            <text>ATP + L-valine + tRNA(Val) = AMP + diphosphate + L-valyl-tRNA(Val)</text>
            <dbReference type="Rhea" id="RHEA:10704"/>
            <dbReference type="Rhea" id="RHEA-COMP:9672"/>
            <dbReference type="Rhea" id="RHEA-COMP:9708"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57762"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78537"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.9"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="8">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="8">The C-terminal coiled-coil domain is crucial for aminoacylation activity.</text>
    </comment>
    <comment type="domain">
        <text evidence="8">ValRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated threonine is translocated from the active site to the editing site.</text>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the class-I aminoacyl-tRNA synthetase family. ValS type 1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.9" evidence="8"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09507.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517206.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2630"/>
    <dbReference type="EnsemblBacteria" id="ABF09507">
        <property type="protein sequence ID" value="ABF09507"/>
        <property type="gene ID" value="Rmet_2630"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2630"/>
    <dbReference type="eggNOG" id="COG0525">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001493_0_2_4"/>
    <dbReference type="OMA" id="FATKLWN"/>
    <dbReference type="OrthoDB" id="32262at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002161">
        <property type="term" value="F:aminoacyl-tRNA editing activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004832">
        <property type="term" value="F:valine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006438">
        <property type="term" value="P:valyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd07962">
        <property type="entry name" value="Anticodon_Ia_Val"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.380">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.740.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02004">
        <property type="entry name" value="Val_tRNA_synth_type1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001412">
        <property type="entry name" value="aa-tRNA-synth_I_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002300">
        <property type="entry name" value="aa-tRNA-synth_Ia"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033705">
        <property type="entry name" value="Anticodon_Ia_Val"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013155">
        <property type="entry name" value="M/V/L/I-tRNA-synth_anticd-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010978">
        <property type="entry name" value="tRNA-bd_arm"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009080">
        <property type="entry name" value="tRNAsynth_Ia_anticodon-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037118">
        <property type="entry name" value="Val-tRNA_synth_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019499">
        <property type="entry name" value="Val-tRNA_synth_tRNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009008">
        <property type="entry name" value="Val/Leu/Ile-tRNA-synth_edit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002303">
        <property type="entry name" value="Valyl-tRNA_ligase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11946">
        <property type="entry name" value="PTHR11946"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08264">
        <property type="entry name" value="Anticodon_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00133">
        <property type="entry name" value="tRNA-synt_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10458">
        <property type="entry name" value="Val_tRNA-synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00986">
        <property type="entry name" value="TRNASYNTHVAL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46589">
        <property type="entry name" value="SSF46589"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47323">
        <property type="entry name" value="SSF47323"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50677">
        <property type="entry name" value="SSF50677"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00422">
        <property type="entry name" value="valS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00178">
        <property type="entry name" value="AA_TRNA_LIGASE_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8 9" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="5 8 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2 8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 8 9" id="KW-0436">Ligase</keyword>
    <keyword evidence="4 8 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6 8 9" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="tRNA-synt_1" evidence="10">
        <location>
            <begin position="24"/>
            <end position="626"/>
        </location>
    </feature>
    <feature type="domain" description="Anticodon_1" evidence="11">
        <location>
            <begin position="677"/>
            <end position="830"/>
        </location>
    </feature>
    <feature type="domain" description="Val_tRNA-synt_C" evidence="12">
        <location>
            <begin position="889"/>
            <end position="953"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'HIGH' region" evidence="8">
        <location>
            <begin position="49"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'KMSKS' region" evidence="8">
        <location>
            <begin position="549"/>
            <end position="553"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="552"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001624"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU363035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF08264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF10458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ABF09507.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="955" mass="106728" checksum="C23D099BDD63C125" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LK36</accession>
    <name>Q1LK36_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Alkaline phosphatase</fullName>
            <ecNumber evidence="4">3.1.3.1</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Rmet_2613</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EC" id="3.1.3.1" evidence="4"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09490.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517193.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2613"/>
    <dbReference type="EnsemblBacteria" id="ABF09490">
        <property type="protein sequence ID" value="ABF09490"/>
        <property type="gene ID" value="Rmet_2613"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2613"/>
    <dbReference type="eggNOG" id="COG3540">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015982_2_0_4"/>
    <dbReference type="OMA" id="EFSDDCW"/>
    <dbReference type="OrthoDB" id="165142at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004035">
        <property type="term" value="F:alkaline phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd07389">
        <property type="entry name" value="MPP_PhoD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018946">
        <property type="entry name" value="PhoD-like_MPP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038607">
        <property type="entry name" value="PhoD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032093">
        <property type="entry name" value="PhoD_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09423">
        <property type="entry name" value="PhoD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16655">
        <property type="entry name" value="PhoD_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="SSF56300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PhoD_N" evidence="3">
        <location>
            <begin position="57"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="domain" description="PhoD" evidence="2">
        <location>
            <begin position="159"/>
            <end position="640"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="26"/>
            <end position="67"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF09423"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF16655"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF09490.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="679" mass="73192" checksum="ACC54B35EB272A6D" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-12-02" version="59" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LK89</accession>
    <name>Q1LK89_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_2560</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09437.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2560"/>
    <dbReference type="EnsemblBacteria" id="ABF09437">
        <property type="protein sequence ID" value="ABF09437"/>
        <property type="gene ID" value="Rmet_2560"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2560"/>
    <dbReference type="eggNOG" id="ENOG50336R2">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_936390_0_0_4"/>
    <dbReference type="OMA" id="QHRRETT"/>
    <dbReference type="OrthoDB" id="1654294at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043733">
        <property type="entry name" value="DUF5677"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18928">
        <property type="entry name" value="DUF5677"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF09437.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="305" mass="34140" checksum="67A2D7C86C841718" modified="2006-05-30" version="1">MGARVSEQAEGQEIPDIKRVEVSPEELAKFTDEEDFTGLSVDLMIEQGSWTCLTASLLPGETRKWDRDQAILGGLLVRFYKLASALLDQTCQHRRETTFVLGRLAIECMINIQYLVSTNSKAVYQAYVIDSLRHEKKLMDRIETNIASRSGVMLPIEARMLGSIQKSFDKSGVKPEEVTKQAAKPWKDVDLYQRADAVGLGEAYLGLFGGPSHNVHGSWQDLIEYHLHHDGEGFTPELAWHRPRPQVLFTLARIGVETLMAYLEHFAGDGAEGVISELQDLGDRIEIANRAHEAFLSARQSKETA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LKB2</accession>
    <name>Q1LKB2_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_2537</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09414.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517113.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2537"/>
    <dbReference type="EnsemblBacteria" id="ABF09414">
        <property type="protein sequence ID" value="ABF09414"/>
        <property type="gene ID" value="Rmet_2537"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2537"/>
    <dbReference type="HOGENOM" id="CLU_1183441_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF09414.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="249" mass="27775" checksum="D16730F2B6AC120E" modified="2006-05-30" version="1">MRRSTYADSARDCATAQRGRLQPRLSALIAEVRALRRELELMLALYAMPDPREVMACRAQLLDQWIQQAQGRRQLVCLACAAEEGVLTRHHLELGQQTASTTSDRALRALYWASVQRWSRLPVAGQPRTSAPVVRRYASKIVAADMCEKCCELRHDARRFAPHEALAPLAPLALEHDEMHRTDQAVTMSRYDCMACATRWVRRVLPWEPFVSWSVVQSDVASAATPPGPLHALTAMAVGKHSGLVPSSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LKK4</accession>
    <name>Q1LKK4_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">DNA recombination protein RmuC-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Rmet_2445</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in DNA recombination.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the RmuC family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09322.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517038.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2445"/>
    <dbReference type="EnsemblBacteria" id="ABF09322">
        <property type="protein sequence ID" value="ABF09322"/>
        <property type="gene ID" value="Rmet_2445"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2445"/>
    <dbReference type="eggNOG" id="COG1322">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020365_1_1_4"/>
    <dbReference type="OMA" id="GDSKMQG"/>
    <dbReference type="OrthoDB" id="1309809at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003798">
        <property type="entry name" value="DNA_recombination_RmuC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30563">
        <property type="entry name" value="PTHR30563"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02646">
        <property type="entry name" value="RmuC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="110"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="384"/>
            <end position="418"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003416"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF09322.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="448" mass="49994" checksum="1935C3E4DB4AB3A0" modified="2006-05-30" version="1">MSLVSILTLATAVVAVLLLLVLVWRTQRRSAGADLQPLLGELRDDVREDTARGLERLEREVRTEIAESARANRGEAGQQMLRFQQTLSTHLTSIATVQQQQLAQLSEANERRLVEVRATLEQKLRDIEANNAAKLEEMRRTVDEKLHATLEQRLGESFKLVSDRLEQVHRGLGEMQVLAQGVGDLKRVLTNVKSRGTWGEVQLEMLLEQILTPDQYAKNVETVRSSGARVEFAIRLPGRELESGAVWLPIDAKFPKEQYERLIDAQDRGDVDGVNSASRELEVAVRREAQTISEKYLSPPATTDFAILFLPTEGLYAEVLRRPGLTDLLQRDYRVTVAGPTTLTALLNSLQMGFRTLALEKRSSEVWEVLGAVKTEFGKFGEVLAKTRDTLVRAARNIEQAEVRTRQMERKLRTVEALPGDLAEQLLGTAEKASVDADAAEQGQAEGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LKL1</accession>
    <name>Q1LKL1_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Phosphoglycolate phosphatase</fullName>
            <ecNumber evidence="1">3.1.3.18</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">gph</name>
        <name evidence="1" type="ordered locus">Rmet_2438</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EC" id="3.1.3.18" evidence="1"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09315.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517032.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2438"/>
    <dbReference type="EnsemblBacteria" id="ABF09315">
        <property type="protein sequence ID" value="ABF09315"/>
        <property type="gene ID" value="Rmet_2438"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2438"/>
    <dbReference type="eggNOG" id="COG0546">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045011_19_2_4"/>
    <dbReference type="OMA" id="LLIFDWD"/>
    <dbReference type="OrthoDB" id="1484726at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008967">
        <property type="term" value="F:phosphoglycolate phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.240">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006439">
        <property type="entry name" value="HAD-SF_hydro_IA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041492">
        <property type="entry name" value="HAD_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023198">
        <property type="entry name" value="PGP-like_dom2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13419">
        <property type="entry name" value="HAD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="SSF56784"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01549">
        <property type="entry name" value="HAD-SF-IA-v1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01509">
        <property type="entry name" value="HAD-SF-IA-v3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF09315.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="219" mass="23823" checksum="240875EA6ABFE891" modified="2006-05-30" version="1">MARQRFDLIVFDWDGTLMDSTPTIAKCIQLASRDLGLPVPDDSAASHVIGLGLKDALSYAVPTLDPADYPRLAERYRYHFLTRDAELVLFPGVLDMLEALHAEHYFLGVATGKTRVGLERALAATGIGKLFDATRCADETFSKPHPAMLHELTRELGQDIGRTVMIGDTTHDLQMAINAGAQGLGVTYGAHPAESLREMSPVHCATSISDLQAWLLAHA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-12-12" modified="2022-05-25" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LKL5</accession>
    <name>NTPPB_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">7-methyl-GTP pyrophosphatase</fullName>
            <shortName evidence="1">m(7)GTP pyrophosphatase</shortName>
            <ecNumber evidence="1">3.6.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">Rmet_2434</name>
    </gene>
    <organism>
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Nucleoside triphosphate pyrophosphatase that hydrolyzes 7-methyl-GTP (m(7)GTP). May have a dual role in cell division arrest and in preventing the incorporation of modified nucleotides into cellular nucleic acids.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>H2O + N(7)-methyl-GTP = diphosphate + H(+) + N(7)-methyl-GMP</text>
            <dbReference type="Rhea" id="RHEA:58744"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58285"/>
            <dbReference type="ChEBI" id="CHEBI:87133"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the Maf family. YceF subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.1.-" evidence="1"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09311.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517028.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q1LKL5"/>
    <dbReference type="SMR" id="Q1LKL5"/>
    <dbReference type="STRING" id="266264.Rmet_2434"/>
    <dbReference type="EnsemblBacteria" id="ABF09311">
        <property type="protein sequence ID" value="ABF09311"/>
        <property type="gene ID" value="Rmet_2434"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2434"/>
    <dbReference type="eggNOG" id="COG0424">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_040416_1_0_4"/>
    <dbReference type="OMA" id="CAGSFKA"/>
    <dbReference type="OrthoDB" id="1469203at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047429">
        <property type="term" value="F:nucleoside-triphosphate diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009117">
        <property type="term" value="P:nucleotide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00555">
        <property type="entry name" value="Maf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.950.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00528">
        <property type="entry name" value="Maf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029001">
        <property type="entry name" value="ITPase-like_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003697">
        <property type="entry name" value="Maf-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43213">
        <property type="entry name" value="PTHR43213"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02545">
        <property type="entry name" value="Maf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006305">
        <property type="entry name" value="Maf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52972">
        <property type="entry name" value="SSF52972"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00172">
        <property type="entry name" value="maf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0546">Nucleotide metabolism</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000267390" description="7-methyl-GTP pyrophosphatase">
        <location>
            <begin position="1"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="1">
        <location>
            <position position="74"/>
        </location>
    </feature>
    <feature type="site" description="Important for substrate specificity" evidence="1">
        <location>
            <position position="17"/>
        </location>
    </feature>
    <feature type="site" description="Important for substrate specificity" evidence="1">
        <location>
            <position position="75"/>
        </location>
    </feature>
    <feature type="site" description="Important for substrate specificity" evidence="1">
        <location>
            <position position="159"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00528"/>
        </source>
    </evidence>
    <sequence length="199" mass="21260" checksum="2284AA883F812A2E" modified="2006-05-30" version="1">MSSDTRPALILGSSSPYRRELLSRLRIPFEVATPDIDETPLAGERPAATALRLSRLKAEAIAALHAGALVIGSDQVCTLDDQQIGKPGTHAKALAQLQLMRGRTVTFHSALCLLDGRTGEAQIADIQTHATFRNLSDAELDAYLRLETPYDCAGSAKVEGLGIMLLERVESDDPTALIGLPLIALTGMLRHAGYPCFGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LKW9</accession>
    <name>Q1LKW9_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative plasmid-related DNA/RNA helicase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_2330</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09207.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004350563.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2330"/>
    <dbReference type="EnsemblBacteria" id="ABF09207">
        <property type="protein sequence ID" value="ABF09207"/>
        <property type="gene ID" value="Rmet_2330"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2330"/>
    <dbReference type="eggNOG" id="COG0553">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010309_1_0_4"/>
    <dbReference type="OMA" id="IYLGYAN"/>
    <dbReference type="OrthoDB" id="145808at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10810">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038718">
        <property type="entry name" value="SNF2-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 3" id="KW-0347">Helicase</keyword>
    <keyword evidence="1 3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase C-terminal" evidence="2">
        <location>
            <begin position="581"/>
            <end position="741"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF09207.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="759" mass="83260" checksum="F8F40B97B63D4A35" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="54" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LKX1</accession>
    <name>Q1LKX1_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_2328</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09205.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_003116818.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2328"/>
    <dbReference type="EnsemblBacteria" id="ABF09205">
        <property type="protein sequence ID" value="ABF09205"/>
        <property type="gene ID" value="Rmet_2328"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2328"/>
    <dbReference type="eggNOG" id="ENOG5030A37">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_162353_2_0_4"/>
    <dbReference type="OMA" id="VCWLASK"/>
    <dbReference type="OrthoDB" id="2053795at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF09205.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="106" mass="12172" checksum="C00384DC967CBEE3" modified="2006-05-30" version="1">MIPNPFKRPAPHKQPLFAPSTLKLSEKVHWLARRGLIDPLAYVQRHVRGDWGEIDEATRQANDVAIQQDNLMISQFRITPDLALIVKTSEDHQTTVVQLPEERDLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LL99</accession>
    <name>Q1LL99_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Acid-CoA ligase</fullName>
            <ecNumber evidence="3">6.2.1.26</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_2198</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EC" id="6.2.1.26" evidence="3"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09077.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516905.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2198"/>
    <dbReference type="EnsemblBacteria" id="ABF09077">
        <property type="protein sequence ID" value="ABF09077"/>
        <property type="gene ID" value="Rmet_2198"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2198"/>
    <dbReference type="eggNOG" id="COG0365">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000022_59_10_4"/>
    <dbReference type="OMA" id="GPVGCRY"/>
    <dbReference type="OrthoDB" id="326708at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008756">
        <property type="term" value="F:o-succinylbenzoate-CoA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025110">
        <property type="entry name" value="AMP-bd_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045851">
        <property type="entry name" value="AMP-bd_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020845">
        <property type="entry name" value="AMP-binding_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_Synth/Lig"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13193">
        <property type="entry name" value="AMP-binding_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00455">
        <property type="entry name" value="AMP_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0436">Ligase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AMP-binding" evidence="1">
        <location>
            <begin position="50"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="domain" description="AMP-binding_C" evidence="2">
        <location>
            <begin position="461"/>
            <end position="539"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF09077.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="553" mass="60307" checksum="BF4D7C017A45C65A" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-10-07" version="50" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LLC9</accession>
    <name>Q1LLC9_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_2168</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09047.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2168"/>
    <dbReference type="EnsemblBacteria" id="ABF09047">
        <property type="protein sequence ID" value="ABF09047"/>
        <property type="gene ID" value="Rmet_2168"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2168"/>
    <dbReference type="HOGENOM" id="CLU_2555805_0_0_4"/>
    <dbReference type="OMA" id="EPANCPL"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF09047.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="82" mass="8655" checksum="8832783B5E83EE73" modified="2006-05-30" version="1">MLPEPANCRLCGSASERIRSSKLRGFRYTCPSCGSFGIESAALAGLGANASIQQDLAHLRAYGLLPLIHRDKQGVSVGPGRV</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-01-15" modified="2022-08-03" version="100" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LLJ5</accession>
    <name>DER_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">GTPase Der</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">GTP-binding protein EngA</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">der</name>
        <name type="synonym">engA</name>
        <name type="ordered locus">Rmet_2102</name>
    </gene>
    <organism>
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">GTPase that plays an essential role in the late steps of ribosome biogenesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Associates with the 50S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily. EngA (Der) GTPase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08981.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516815.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q1LLJ5"/>
    <dbReference type="SMR" id="Q1LLJ5"/>
    <dbReference type="STRING" id="266264.Rmet_2102"/>
    <dbReference type="EnsemblBacteria" id="ABF08981">
        <property type="protein sequence ID" value="ABF08981"/>
        <property type="gene ID" value="Rmet_2102"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2102"/>
    <dbReference type="eggNOG" id="COG1160">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016077_6_2_4"/>
    <dbReference type="OMA" id="KFRFLEY"/>
    <dbReference type="OrthoDB" id="263682at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042254">
        <property type="term" value="P:ribosome biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00195">
        <property type="entry name" value="GTPase_Der"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031166">
        <property type="entry name" value="G_ENGA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016484">
        <property type="entry name" value="GTP-bd_EngA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032859">
        <property type="entry name" value="KH_dom-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015946">
        <property type="entry name" value="KH_dom-like_a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14714">
        <property type="entry name" value="KH_dom-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01926">
        <property type="entry name" value="MMR_HSR1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006485">
        <property type="entry name" value="GTP-binding_EngA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00326">
        <property type="entry name" value="GTP1OBG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03594">
        <property type="entry name" value="GTPase_EngA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51712">
        <property type="entry name" value="G_ENGA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0342">GTP-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0677">Repeat</keyword>
    <keyword id="KW-0690">Ribosome biogenesis</keyword>
    <feature type="chain" id="PRO_1000011712" description="GTPase Der">
        <location>
            <begin position="1"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="domain" description="EngA-type G 1">
        <location>
            <begin position="3"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="domain" description="EngA-type G 2">
        <location>
            <begin position="181"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="domain" description="KH-like" evidence="1">
        <location>
            <begin position="355"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="9"/>
            <end position="16"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="56"/>
            <end position="60"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="119"/>
            <end position="122"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="187"/>
            <end position="194"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="234"/>
            <end position="238"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="299"/>
            <end position="302"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00195"/>
        </source>
    </evidence>
    <sequence length="447" mass="49323" checksum="AFADFA1EC0EDA542" modified="2006-05-30" version="1">MKPVIALVGRPNVGKSTLFNRMTRSRDALVADLPGLTRDRHYGEGRIGDRPFIVIDTGGFEPVAKEGIVAEMAKQTRQAVVEADVVIFLVDGRLGLAPQDRVIADYLRKTGRRVMLAINKAEGMKYTSVAADFYELGMGDPYAISSTHGDGVRELVDEALDLAVQERPELADQEDAGQHGVKIAIVGRPNVGKSTLVNTLIGEERVIAFDMPGTTRDAIYVEFERGGKPYTLIDTAGLRKRGKVFEAIEKFSVVKTLQSIADANVVVLLLDAQQDISEQDAHIAGFIVESGRALVVGVNKWDGLDGHQRDRVKHDLERKLQFLSFANIHFVSARERTGIGALLRSVDDAYAAAMIKLPTPQITRVLQEAVEFQQPKRVGVSRPKLRYAHQGGSNPPIIVVHGNALSGVTDAYRRYLENRFRTAFKLKGTPLRIEFRTNKNPYAESKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-10-07" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LLS9</accession>
    <name>Q1LLS9_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_2018</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08897.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2018"/>
    <dbReference type="EnsemblBacteria" id="ABF08897">
        <property type="protein sequence ID" value="ABF08897"/>
        <property type="gene ID" value="Rmet_2018"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2018"/>
    <dbReference type="HOGENOM" id="CLU_2275136_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193606" evidence="1">
        <location>
            <begin position="20"/>
            <end position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF08897.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="102" mass="11142" checksum="364D11E9D1671EB9" modified="2006-05-30" version="1">MTHSFLALLLATAVATAYASDGDYFASAPTWGGSHIDLYKLPCKSGLGDKIAKQWGGSTPPREGCYSIIGQTVQINWPQADGKTKTHTYYLHQFKDPDDIAY</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LLW0</accession>
    <name>Q1LLW0_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_1987</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08866.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF08866">
        <property type="protein sequence ID" value="ABF08866"/>
        <property type="gene ID" value="Rmet_1987"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1987"/>
    <dbReference type="HOGENOM" id="CLU_2635494_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="14"/>
            <end position="33"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF08866.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="77" mass="8668" checksum="FD0E0F55B67C2832" modified="2006-05-30" version="1">MADGTRTHDDRNHNPGLYQLSYSHRRDSSLPTLPLSEEQDYTCLLILWLVSRTKNSTTLPDGFIDFPATLPPAAQLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LLZ8</accession>
    <name>Q1LLZ8_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">General substrate transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Rmet_1949</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08828.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516677.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1949"/>
    <dbReference type="EnsemblBacteria" id="ABF08828">
        <property type="protein sequence ID" value="ABF08828"/>
        <property type="gene ID" value="Rmet_1949"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1949"/>
    <dbReference type="eggNOG" id="COG0477">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001265_39_0_4"/>
    <dbReference type="OMA" id="LLMVELW"/>
    <dbReference type="OrthoDB" id="961781at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="SSF103473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00216">
        <property type="entry name" value="SUGAR_TRANSPORT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00217">
        <property type="entry name" value="SUGAR_TRANSPORT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="21"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="49"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="85"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="115"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="161"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="185"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="234"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="274"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="303"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="336"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="368"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="396"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="domain" description="MFS" evidence="6">
        <location>
            <begin position="13"/>
            <end position="424"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABF08828.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="429" mass="46684" checksum="E75A25EE3067A42C" modified="2006-05-30" version="1">MSSTTQSQHGFRTVFRVVSGNFLEMYDFMVYGFYAAAIARTFFPSDNEFASLMLSLATFGAGFLMRPLGAIILGAYIDHHGRRKGLILTLILMAAGTLLIAVVPGYAAIGVAAPLLVLFGRLLQGFSAGVELGGVSVYLSEIAKPGKKGFYVAWQSASQQVAVIFAGLLGVILHATLAPAQMDSWGWRVPFLIGCLIVPFLFIIRRSLEETEEFRTRKHRPSVNEIYRSMLDNWGIIIAGCMMVVMTTVSFYMITAYTPTFGKTVLKLDDVDNLIVTMCVGLSNFIWLPLMGAVSDRIGRKPLLVFFTIATLVTAYPAVSWLVAAPSFERLLMVELWLSFLYGSYNGAMVVTLTEVMPPAVRTAGFSLAYSLATALFGGFTPAISTYLIHATGNKAAPGLWLMFAAACGLVATIIIFRTRREQSALSAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LM02</accession>
    <name>Q1LM02_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Amidase, hydantoinase/carbamoylase (Family)</fullName>
            <ecNumber evidence="4">3.5.1.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Rmet_1945</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="2">Binds 2 Zn(2+) ions per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase M20 family.</text>
    </comment>
    <dbReference type="EC" id="3.5.1.-" evidence="4"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08824.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516673.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1945"/>
    <dbReference type="EnsemblBacteria" id="ABF08824">
        <property type="protein sequence ID" value="ABF08824"/>
        <property type="gene ID" value="Rmet_1945"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1945"/>
    <dbReference type="eggNOG" id="COG0624">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024588_6_1_4"/>
    <dbReference type="OMA" id="GHDAMMM"/>
    <dbReference type="OrthoDB" id="829830at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016813">
        <property type="term" value="F:hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amidines"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010158">
        <property type="entry name" value="Amidase_Cbmase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036264">
        <property type="entry name" value="Bact_exopeptidase_dim_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002933">
        <property type="entry name" value="Peptidase_M20"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32494">
        <property type="entry name" value="PTHR32494"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01546">
        <property type="entry name" value="Peptidase_M20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001235">
        <property type="entry name" value="Amidase_carbamoylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55031">
        <property type="entry name" value="SSF55031"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01879">
        <property type="entry name" value="hydantase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0862">Zinc</keyword>
    <feature type="binding site" evidence="2">
        <location>
            <position position="85"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="96"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="131"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="199"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="224"/>
        </location>
        <ligand>
            <name>allantoate</name>
            <dbReference type="ChEBI" id="CHEBI:17536"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="283"/>
        </location>
        <ligand>
            <name>allantoate</name>
            <dbReference type="ChEBI" id="CHEBI:17536"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="296"/>
        </location>
        <ligand>
            <name>allantoate</name>
            <dbReference type="ChEBI" id="CHEBI:17536"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="391"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006153"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR001235-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR001235-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF08824.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="420" mass="44748" checksum="2B58177D022FD14B" modified="2006-05-30" version="1">MTIATPSLGQSILERAEALARFSDMEDGLTCAFLTPAHRQAQAQLARWMEDAGMTVRIDAIGNVIGRYAADSATANPKVLMTGSHFDTVRNGGRYDGRLGILLPIAVVGALRDAGIRLPYHFEVVGFAEEEGLRFKTSFLASSVLAGRFDPALLDRADVDGVTMREALADSGLPGAGDIDALRAAAVDPSTLAGFVEVHIEQGPVLLNRGLPLGIVTQIAGSSRFQVRAEGLASHAGTTPMDMRRDAATGAAEMILLVESRCAQVPTLVGTVGQLQVPNGSSNVIPAACVFSMDIRAGEDGIREAAIADIVAGIERIAAKRSLKASVERVTPVNNAPCARWLMDQFGAVLKKRGHEVFELPSGAGHDAMMMHRIIDVAMLFVRCGNGGISHNPLETITEEDAQQAAEVFVDFLRHFRVKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LMC9</accession>
    <name>Q1LMC9_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Transcriptional regulator (IclR family)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Rmet_1818</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08697.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516547.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1818"/>
    <dbReference type="EnsemblBacteria" id="ABF08697">
        <property type="protein sequence ID" value="ABF08697"/>
        <property type="gene ID" value="Rmet_1818"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1818"/>
    <dbReference type="eggNOG" id="COG1414">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_062618_0_0_4"/>
    <dbReference type="OMA" id="ARHYETH"/>
    <dbReference type="OrthoDB" id="370329at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029016">
        <property type="entry name" value="GAF-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014757">
        <property type="entry name" value="Tscrpt_reg_IclR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005471">
        <property type="entry name" value="Tscrpt_reg_IclR_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09339">
        <property type="entry name" value="HTH_IclR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00346">
        <property type="entry name" value="HTH_ICLR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51077">
        <property type="entry name" value="HTH_ICLR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51078">
        <property type="entry name" value="ICLR_ED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH iclR-type" evidence="3">
        <location>
            <begin position="17"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="domain" description="IclR-ED" evidence="4">
        <location>
            <begin position="80"/>
            <end position="263"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABF08697.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="264" mass="28841" checksum="4AAFAB58FE547863" modified="2006-05-30" version="1">MSATDIPPDDARDRQFATTLANGLDILLAYRAGESLLGNKDFVQRTGLSKSTVARLTHTLSALGYLRHDTALGKYRLGAPVLAPGYPLLASMPIRQIARPLMKALADEVGGAVSLGIRDRTQMIYVETARSTDNLVVPPDIGAALPMLTTAIGKAWLCRAPAELRDPVLNRLRLQDPDAFARGAPGLSQARKDLERLGYCTNRAEWRHDVFGFAVPLCKPVDSQWFVINSGIRAQPGRFHAVEQFVGPRLVTLAHNLEVQLGLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LMH7</accession>
    <name>Q1LMH7_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Asp-tRNA Asn / Glu-tRNA Gln amidotransferase A subunit or related amidase</fullName>
            <ecNumber evidence="2">6.3.5.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">gatA</name>
        <name evidence="2" type="ordered locus">Rmet_1770</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EC" id="6.3.5.-" evidence="2"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08649.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516502.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1770"/>
    <dbReference type="EnsemblBacteria" id="ABF08649">
        <property type="protein sequence ID" value="ABF08649"/>
        <property type="gene ID" value="Rmet_1770"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1770"/>
    <dbReference type="eggNOG" id="COG0154">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009600_0_4_4"/>
    <dbReference type="OMA" id="HSLFTKE"/>
    <dbReference type="OrthoDB" id="1239251at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016874">
        <property type="term" value="F:ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1300.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000120">
        <property type="entry name" value="Amidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023631">
        <property type="entry name" value="Amidase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036928">
        <property type="entry name" value="AS_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11895">
        <property type="entry name" value="PTHR11895"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01425">
        <property type="entry name" value="Amidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75304">
        <property type="entry name" value="SSF75304"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0436">Ligase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Amidase" evidence="1">
        <location>
            <begin position="26"/>
            <end position="456"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF08649.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="475" mass="51194" checksum="B322CB8FA0A56FD2" modified="2006-05-30" version="1">MSESLCFLSATELADAIRHKRVSPVDITSAVLARAERLQPELNCFITIDAERAMESARQAERLVMAGGPLGLLHGVPFTVKDIVNTQGVRTTFGAAPLRDNVPQQDAVAVARLREQGAILIGKTTTPEFGSKCLTDSPLFGRTRNAWSAQRTSGGSSGGAAVAVASGIAPLAVATDGGGSTRIPAACNGVVGIKQSNGVIPHSQAQDLYGNQTYVTPTTRTVADTGLMMAAMAGEHACDPWSIGVPKADYVDAARAQGDLRGKRILYCLAPPGRPVAADVARAFERGLAALAGLGAELEPFDGDGFNDIEPLWRAINHTVWRTRFVEIVERHRDELSATFVRQVESAAQVSGVAYQQAMFDRSRLFQRVQALLERADLLAMPTIMRTALPIDQDLFGTIEIDGHVHENVRANWFPWTMPFNMTGHPAISLPCGFGSDDLPIGLQLVGRFRRDTELLRCAALFESAHGFLDRWPSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="59" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LMK0</accession>
    <name>Q1LMK0_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_1747</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08626.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516478.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1747"/>
    <dbReference type="EnsemblBacteria" id="ABF08626">
        <property type="protein sequence ID" value="ABF08626"/>
        <property type="gene ID" value="Rmet_1747"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1747"/>
    <dbReference type="eggNOG" id="COG0645">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_105030_3_0_4"/>
    <dbReference type="OMA" id="HMVCGKI"/>
    <dbReference type="OrthoDB" id="1574819at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF08626.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="177" mass="19726" checksum="A4A1593C5214863B" modified="2006-05-30" version="1">MDQETNIVACSDTAALHMVCGKIASGKSSLTARLAAAPRRVLLSEDKWIACLYPDEIHTLSDYMNRTARIRTLLTDHIVSLLTAGTSVVLDFPFNTVGARTWGASLYRAAGCEHRLHYLEVSDEICKSRLKARNLSGEHPFQTSEEQFDQITSYFVPPAHGEGFNLITYDETGKVRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="103" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LML5</accession>
    <name>Q1LML5_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Transcriptional regulator, AraC family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Rmet_1732</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08611.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516464.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1732"/>
    <dbReference type="EnsemblBacteria" id="ABF08611">
        <property type="protein sequence ID" value="ABF08611"/>
        <property type="gene ID" value="Rmet_1732"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1732"/>
    <dbReference type="eggNOG" id="COG2207">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047522_1_2_4"/>
    <dbReference type="OMA" id="IYVRTCR"/>
    <dbReference type="OrthoDB" id="1622875at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032687">
        <property type="entry name" value="AraC-type_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018060">
        <property type="entry name" value="HTH_AraC"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12625">
        <property type="entry name" value="Arabinose_bd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12833">
        <property type="entry name" value="HTH_18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00342">
        <property type="entry name" value="HTH_ARAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01124">
        <property type="entry name" value="HTH_ARAC_FAMILY_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH araC/xylS-type" evidence="4">
        <location>
            <begin position="233"/>
            <end position="331"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS01124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABF08611.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="340" mass="37915" checksum="E1F00B3135193806" modified="2006-05-30" version="1">MSYFLRSASLTNYMEVARAVGLDPHQMLRAAKINRDVLLDPDIRIPAASVGRLLEASARDGHAEDFGLRLAELRQFSNLGPLAFVVREEPTLRRALESMVRYMGLQNESLAMRVEEMDGLVVIRLQILSDQPAGSMRQATQLAAGVMFRMLSMFLGPAWRPRSICFSHAPPESQTTYLRVFGMPALFNQDFDGIVCVASDLEAPLPSYDPVMAQQVKRYLGTLLAQSNTTMADKVRKLVYALLPSGMCSIDRVAQHLGVDRRTIHRRLAQDGTTYSQILNEARAGLATSYIENRSRPLSEVATLLGFSSLSAFSRWFGNQFGCSVSKWRTTRTGEQVEET</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LML9</accession>
    <name>Q1LML9_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Extra-cytoplasmic Solute Receptor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">bug</name>
        <name evidence="4" type="ordered locus">Rmet_1726</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Periplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the UPF0065 (bug) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08607.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516460.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1726"/>
    <dbReference type="EnsemblBacteria" id="ABF08607">
        <property type="protein sequence ID" value="ABF08607"/>
        <property type="gene ID" value="Rmet_1726"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1726"/>
    <dbReference type="eggNOG" id="COG3181">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045683_0_0_4"/>
    <dbReference type="OMA" id="TRLVMQK"/>
    <dbReference type="OrthoDB" id="447734at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042597">
        <property type="term" value="C:periplasmic space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005064">
        <property type="entry name" value="BUG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042100">
        <property type="entry name" value="Bug_dom1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42928">
        <property type="entry name" value="PTHR42928"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03401">
        <property type="entry name" value="TctC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017082">
        <property type="entry name" value="YflP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0675">Receptor</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193570" evidence="3">
        <location>
            <begin position="34"/>
            <end position="337"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF08607.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="337" mass="35535" checksum="3D3606C5A26EC734" modified="2006-05-30" version="1">MTAQKDSRRAARRRLVQAAILASGLMLAVPAMAQSASTQNWPSRPVRVVVPYPPGGVSDAVTRLVMQKLAERIGQPVVVENRPGANGMIGSDNVAKSAPDGYSLLVVVAAHAINPSLYQKMSYDPIRDLTGVSEIGRIPLLMVSSAQLPPKSVKELVSWGKAHPDQMTFASSGSGSGAHLAGELFSQGTGMTMTHVPYKGISPALPDLISGQVAVIFDSVQTMIPLAKAGKVRALAITNPRRWPAAPDVPTMAEAGYPNIMPSSWIGVLAPAKTPTAIIDKLSAELDAVVHAPDVQAKLIDYGIDPVGGKADQFQAFIKEEAGRWASVVQKAGIKLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LMN8</accession>
    <name>Q1LMN8_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">PPK2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Rmet_1707</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the polyphosphate kinase 2 (PPK2) family. Class I subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08588.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516440.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1707"/>
    <dbReference type="EnsemblBacteria" id="ABF08588">
        <property type="protein sequence ID" value="ABF08588"/>
        <property type="gene ID" value="Rmet_1707"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1707"/>
    <dbReference type="eggNOG" id="COG2326">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_048699_3_0_4"/>
    <dbReference type="OMA" id="WAPWYVA"/>
    <dbReference type="OrthoDB" id="953793at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008976">
        <property type="term" value="F:polyphosphate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016898">
        <property type="entry name" value="Polyphosphate_phosphotransfera"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022488">
        <property type="entry name" value="PPK2-related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022486">
        <property type="entry name" value="PPK2_PA0141"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03976">
        <property type="entry name" value="PPK2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF028756">
        <property type="entry name" value="PPK2_prd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03707">
        <property type="entry name" value="PPK2_P_aer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PPK2" evidence="4">
        <location>
            <begin position="18"/>
            <end position="241"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009924"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF03976"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABF08588.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="272" mass="31833" checksum="485A5929829E8322" modified="2006-05-30" version="1">MVKDSRRTDEDSAMQQDLDSKTYEKALRSLHVELVHLQDWVKATGAKVCVIFEGRDGAGKGGVIKAITERVSPRVFRVIALPAPTDREKSQMYVQRYIPHLPAAGEIVIFDRSWYNRAGVERVMKFCDEEQVELFLRAVPLVERAIVGSNIQLIKYWLEVSPEEQTRRLEQRILDGRKTWKLTDMDLKSYSRWYDYSRARDAMFEASDTDFAPWNVVRSDCKRRARLNIISHLLASVPYEPIPRKKVTLPDRQKRGSYKEPDYPYRYIPEKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LMR3</accession>
    <name>Q1LMR3_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Permease of the major facilitator superfamily</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Rmet_1682</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08563.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516411.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1682"/>
    <dbReference type="EnsemblBacteria" id="ABF08563">
        <property type="protein sequence ID" value="ABF08563"/>
        <property type="gene ID" value="Rmet_1682"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1682"/>
    <dbReference type="eggNOG" id="COG2814">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001265_59_9_4"/>
    <dbReference type="OMA" id="CGMQLVF"/>
    <dbReference type="OrthoDB" id="1594866at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="SSF103473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="42"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="75"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="100"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="139"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="165"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="221"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="259"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="284"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="311"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="345"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="380"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="domain" description="MFS" evidence="6">
        <location>
            <begin position="10"/>
            <end position="404"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABF08563.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="411" mass="43170" checksum="09438AB4BCC8CD7D" modified="2006-05-30" version="1">MSSRSSLRPVLPILIGASVMLSLAMGLRQSLGIFVPPLTRDIGITVGDFTIAVAVQNLTWGLLQPFAGALAVRTGFRPLMMGGSLLYLIGLVLLATANGLWAVVIGAGVLIGMAMATTGTALAMAAASSAVSQNVRSMILGLVSASGSIGAMIAAPLGQGITGEWGWRMGVAAFAVLAVVMLPAAWFAGRADKVREAPAARAQPEQSGRQALMTALRHPPFVVMALAYTVCGMQLVFLTTHLPAYLEVCGMDPMLSAKALGLIGGFNILGSLFFGWAGGRVNKLLLLGGIYICRSIGFIWFFHALPTPESTMMFSAIMGFLWLGVSPLVQGWIAQTFGLRWQAMIAGVAFFSHQIGSFIGAFGGGWLYDQMQSYSLAWKVGASLGLTLGLIQVAFAFASRPRAPRLVPAAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LMV3</accession>
    <name>Q1LMV3_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">ABC-type transporter, periplasmic component possible urea/short-chain amide ABC transporter up with Au</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_1640</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08523.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516368.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1640"/>
    <dbReference type="EnsemblBacteria" id="ABF08523">
        <property type="protein sequence ID" value="ABF08523"/>
        <property type="gene ID" value="Rmet_1640"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1640"/>
    <dbReference type="eggNOG" id="COG0683">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027128_1_0_4"/>
    <dbReference type="OMA" id="NDATRAW"/>
    <dbReference type="OrthoDB" id="776281at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028081">
        <property type="entry name" value="Leu-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028082">
        <property type="entry name" value="Peripla_BP_I"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13458">
        <property type="entry name" value="Peripla_BP_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53822">
        <property type="entry name" value="SSF53822"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193584" evidence="1">
        <location>
            <begin position="28"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="domain" description="Peripla_BP_6" evidence="2">
        <location>
            <begin position="38"/>
            <end position="375"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF08523.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="412" mass="44566" checksum="BF13DC9D55779210" modified="2006-05-30" version="1">MKTKAQKLAAAVLVTAAFTSVAAHVHAQPKPGISDDIVKIGLLLDMSGLYSDVTGRGSAAAAQMAIDDFGGKVLGKKIELVVVDHQNKADIAANKAREWFDQDHVDAILDVAASAPALAVLDVAKQKNKIVVFSGPGTERITNDLCTPVSVHYAYDTYALANTTARAMVERGGKTWFFLTADYAFGHTLQQSATAVVNEAGGRVLGAARHPLNTSDFASYLLQAQASKAQVVGLANAGGDTVNAIKAASEFGLTRNGNQKLAGLLLYINDIHAIGLKTAQGLVLTEAFYWDMNDATRAWSRRYFEKVKKMPNMSQAGVYSSVSHYLKAVQATGTDETAAVMKQMKATPINDFFAKNGRIREDGRMVHDMYLFEVKKPEESKYPWDYYKLLATVPGDQAFIPPSKSQCPLLKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LMZ6</accession>
    <name>Q1LMZ6_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Cupin_2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_1597</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08480.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516327.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1597"/>
    <dbReference type="EnsemblBacteria" id="ABF08480">
        <property type="protein sequence ID" value="ABF08480"/>
        <property type="gene ID" value="Rmet_1597"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1597"/>
    <dbReference type="eggNOG" id="COG0662">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_147397_0_1_4"/>
    <dbReference type="OMA" id="FWYDQDE"/>
    <dbReference type="OrthoDB" id="1928636at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013096">
        <property type="entry name" value="Cupin_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011051">
        <property type="entry name" value="RmlC_Cupin_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07883">
        <property type="entry name" value="Cupin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51182">
        <property type="entry name" value="SSF51182"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cupin_2" evidence="1">
        <location>
            <begin position="45"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF07883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF08480.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="115" mass="12400" checksum="D6DB21436DDA08EB" modified="2006-05-30" version="1">MTATTGNLLRDVPVHASAEIFADLMEWQAAGRVRIERIVSNGQASAPGFWYDSTDDEWVLVISGSAAVEVDDGTTHTLGAGDWLHLPAHCRHRVARTDAAQPTIWLAIHVSPAAT</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-12-12" modified="2022-05-25" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LNE8</accession>
    <name>LPXD_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">UDP-3-O-acylglucosamine N-acyltransferase</fullName>
            <ecNumber evidence="1">2.3.1.191</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">lpxD</name>
        <name type="ordered locus">Rmet_1445</name>
    </gene>
    <organism>
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the N-acylation of UDP-3-O-acylglucosamine using 3-hydroxyacyl-ACP as the acyl donor. Is involved in the biosynthesis of lipid A, a phosphorylated glycolipid that anchors the lipopolysaccharide to the outer membrane of the cell.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a (3R)-hydroxyacyl-[ACP] + a UDP-3-O-[(3R)-3-hydroxyacyl]-alpha-D-glucosamine = a UDP-2-N,3-O-bis[(3R)-3-hydroxyacyl]-alpha-D-glucosamine + H(+) + holo-[ACP]</text>
            <dbReference type="Rhea" id="RHEA:53836"/>
            <dbReference type="Rhea" id="RHEA-COMP:9685"/>
            <dbReference type="Rhea" id="RHEA-COMP:9945"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:64479"/>
            <dbReference type="ChEBI" id="CHEBI:78827"/>
            <dbReference type="ChEBI" id="CHEBI:137740"/>
            <dbReference type="ChEBI" id="CHEBI:137748"/>
            <dbReference type="EC" id="2.3.1.191"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Bacterial outer membrane biogenesis; LPS lipid A biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homotrimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the transferase hexapeptide repeat family. LpxD subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.191" evidence="1"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08328.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516195.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q1LNE8"/>
    <dbReference type="SMR" id="Q1LNE8"/>
    <dbReference type="STRING" id="266264.Rmet_1445"/>
    <dbReference type="EnsemblBacteria" id="ABF08328">
        <property type="protein sequence ID" value="ABF08328"/>
        <property type="gene ID" value="Rmet_1445"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1445"/>
    <dbReference type="eggNOG" id="COG1044">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_049865_0_1_4"/>
    <dbReference type="OMA" id="QIQIAHN"/>
    <dbReference type="OrthoDB" id="1602061at2"/>
    <dbReference type="UniPathway" id="UPA00973"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016410">
        <property type="term" value="F:N-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009245">
        <property type="term" value="P:lipid A biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd03352">
        <property type="entry name" value="LbH_LpxD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00523">
        <property type="entry name" value="LpxD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001451">
        <property type="entry name" value="Hexapep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018357">
        <property type="entry name" value="Hexapep_transf_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007691">
        <property type="entry name" value="LpxD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011004">
        <property type="entry name" value="Trimer_LpxA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020573">
        <property type="entry name" value="UDP_GlcNAc_AcTrfase_non-rep"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43378">
        <property type="entry name" value="PTHR43378"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00132">
        <property type="entry name" value="Hexapep"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04613">
        <property type="entry name" value="LpxD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51161">
        <property type="entry name" value="SSF51161"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01853">
        <property type="entry name" value="lipid_A_lpxD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00101">
        <property type="entry name" value="HEXAPEP_TRANSFERASES"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0012">Acyltransferase</keyword>
    <keyword id="KW-0441">Lipid A biosynthesis</keyword>
    <keyword id="KW-0444">Lipid biosynthesis</keyword>
    <keyword id="KW-0443">Lipid metabolism</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0677">Repeat</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000264420" description="UDP-3-O-acylglucosamine N-acyltransferase">
        <location>
            <begin position="1"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="348"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="1">
        <location>
            <position position="252"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00523"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="369" mass="38502" checksum="51F22128C5CA3979" modified="2006-05-30" version="1">MKTPTLGQLATENGAQVVGDPDLAVVGLAPLDQAGPGDLSFLSNPLYLPQALASAAGAVIVSPADLERIRADGQAEGRNWLVARNPYVCFARVAQRFDRAANADSRTGIDPRASVAPDAVVPASCFIGPNVVIESGARLGERVRILANAFIGASAEIGEDTLIYANVSVYHRCVIGARNILHSGAVIGADGFGFAPDIGPTGVEYVKIPQVGRAVLGNDVEIGANTAVDRGAMADTVIEDGCKIDNQVQIAHNVHVGAHTVIAGTAAVSGSTKIGRFCVIGGAANFSGHLNIADRTTVSGGTSITKSITKPGGHYTSVFPFTSHGEWERNAAIVRGLSKLRERVVQLERRQRGENNAPAQNKQDEEKSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="61" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LNK1</accession>
    <name>Q1LNK1_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_1392</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08275.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1392"/>
    <dbReference type="EnsemblBacteria" id="ABF08275">
        <property type="protein sequence ID" value="ABF08275"/>
        <property type="gene ID" value="Rmet_1392"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1392"/>
    <dbReference type="eggNOG" id="ENOG50313B9">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1675509_0_0_4"/>
    <dbReference type="OMA" id="IEQPDEL"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.530.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015075">
        <property type="entry name" value="AtaL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023393">
        <property type="entry name" value="START-like_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08982">
        <property type="entry name" value="DUF1857"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF08275.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="192" mass="21660" checksum="DA46E7622FCACA25" modified="2006-05-30" version="1">MMHECPPAIDPIRVKSHRRWHARGLIIRTFTIGRIKLRFEHLVEINDPANPTLEPLTADQLWQGLVLRAESPELFMLGLDRAEIVERGDGWIERVLHFGQANIRDRVVFEPRRAVRYESAATAEHAGGTLTMIIETPGPGALLARFIYETTMPNVDASGDDRYAEIVKSAYHEADLDTVRKIREIAATGQLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="61" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LP60</accession>
    <name>Q1LP60_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_1180</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08066.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515960.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1180"/>
    <dbReference type="EnsemblBacteria" id="ABF08066">
        <property type="protein sequence ID" value="ABF08066"/>
        <property type="gene ID" value="Rmet_1180"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1180"/>
    <dbReference type="HOGENOM" id="CLU_2059476_0_0_4"/>
    <dbReference type="OMA" id="YDMPYAS"/>
    <dbReference type="OrthoDB" id="1923053at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="99"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="99"/>
            <end position="113"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF08066.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="131" mass="14218" checksum="068DE227540084A5" modified="2006-05-30" version="1">MEISDLSHDSASNAEVIGWAAASPQVIARVLNTALAAQDLSGFAALLSDIARDRGLKGARGTHQSIYDMPYASLLPRQKHLLADAFDLFLRLGLEFRARGGEPDDLSDDDGLAPLRDMDDDDVIDDEDRHA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-10-31" modified="2022-05-25" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LP78</accession>
    <name>RL35_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">50S ribosomal protein L35</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpmI</name>
        <name type="ordered locus">Rmet_1162</name>
    </gene>
    <organism>
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the bacterial ribosomal protein bL35 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08048.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_006575466.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q1LP78"/>
    <dbReference type="SMR" id="Q1LP78"/>
    <dbReference type="STRING" id="266264.Rmet_1162"/>
    <dbReference type="EnsemblBacteria" id="ABF08048">
        <property type="protein sequence ID" value="ABF08048"/>
        <property type="gene ID" value="Rmet_1162"/>
    </dbReference>
    <dbReference type="GeneID" id="60822032"/>
    <dbReference type="KEGG" id="rme:Rmet_1162"/>
    <dbReference type="eggNOG" id="COG0291">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_169643_1_0_4"/>
    <dbReference type="OMA" id="PKIKTHR"/>
    <dbReference type="OrthoDB" id="2046660at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.410.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00514">
        <property type="entry name" value="Ribosomal_L35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021137">
        <property type="entry name" value="Ribosomal_L35"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018265">
        <property type="entry name" value="Ribosomal_L35_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001706">
        <property type="entry name" value="Ribosomal_L35_non-mt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037229">
        <property type="entry name" value="Ribosomal_L35_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33343">
        <property type="entry name" value="PTHR33343"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01632">
        <property type="entry name" value="Ribosomal_L35p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00064">
        <property type="entry name" value="RIBOSOMALL35"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143034">
        <property type="entry name" value="SSF143034"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00001">
        <property type="entry name" value="rpmI_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00936">
        <property type="entry name" value="RIBOSOMAL_L35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <feature type="chain" id="PRO_0000258733" description="50S ribosomal protein L35">
        <location>
            <begin position="1"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="65"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="65" mass="7549" checksum="23CCBEF295B16867" modified="2006-05-30" version="1">MPKMKTKKSASKRFTARPNGSFKRGQAFKRHILTKKTTKNKRQLRGTQDVHETNLKSVRAMMPYA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="109" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LPH2</accession>
    <name>Q1LPH2_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 9">4-hydroxy-tetrahydrodipicolinate synthase</fullName>
            <shortName evidence="9">HTPA synthase</shortName>
            <ecNumber evidence="4 9">4.3.3.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9 11" type="primary">dapA</name>
        <name evidence="11" type="ordered locus">Rmet_1068</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2 9">Catalyzes the condensation of (S)-aspartate-beta-semialdehyde [(S)-ASA] and pyruvate to 4-hydroxy-tetrahydrodipicolinate (HTPA).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 9">
            <text>L-aspartate 4-semialdehyde + pyruvate = (2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinate + H(+) + H2O</text>
            <dbReference type="Rhea" id="RHEA:34171"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:67139"/>
            <dbReference type="ChEBI" id="CHEBI:537519"/>
            <dbReference type="EC" id="4.3.3.7"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3 9">Amino-acid biosynthesis; L-lysine biosynthesis via DAP pathway; (S)-tetrahydrodipicolinate from L-aspartate: step 3/4.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Homotetramer; dimer of dimers.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9 10">Belongs to the DapA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">Was originally thought to be a dihydrodipicolinate synthase (DHDPS), catalyzing the condensation of (S)-aspartate-beta-semialdehyde [(S)-ASA] and pyruvate to dihydrodipicolinate (DHDP). However, it was shown in E.coli that the product of the enzymatic reaction is not dihydrodipicolinate but in fact (4S)-4-hydroxy-2,3,4,5-tetrahydro-(2S)-dipicolinic acid (HTPA), and that the consecutive dehydration reaction leading to DHDP is not spontaneous but catalyzed by DapB.</text>
    </comment>
    <dbReference type="EC" id="4.3.3.7" evidence="4 9"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07954.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008650073.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1068"/>
    <dbReference type="EnsemblBacteria" id="ABF07954">
        <property type="protein sequence ID" value="ABF07954"/>
        <property type="gene ID" value="Rmet_1068"/>
    </dbReference>
    <dbReference type="GeneID" id="60821929"/>
    <dbReference type="KEGG" id="rme:Rmet_1068"/>
    <dbReference type="eggNOG" id="COG0329">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_049343_7_1_4"/>
    <dbReference type="OMA" id="GMDACVP"/>
    <dbReference type="OrthoDB" id="1438588at2"/>
    <dbReference type="UniPathway" id="UPA00034">
        <property type="reaction ID" value="UER00017"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008840">
        <property type="term" value="F:4-hydroxy-tetrahydrodipicolinate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019877">
        <property type="term" value="P:diaminopimelate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009089">
        <property type="term" value="P:lysine biosynthetic process via diaminopimelate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00950">
        <property type="entry name" value="DHDPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00418">
        <property type="entry name" value="DapA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005263">
        <property type="entry name" value="DapA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002220">
        <property type="entry name" value="DapA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020625">
        <property type="entry name" value="Schiff_base-form_aldolases_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020624">
        <property type="entry name" value="Schiff_base-form_aldolases_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12128">
        <property type="entry name" value="PTHR12128"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00701">
        <property type="entry name" value="DHDPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001365">
        <property type="entry name" value="DHDPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00146">
        <property type="entry name" value="DHPICSNTHASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM01130">
        <property type="entry name" value="DHDPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00674">
        <property type="entry name" value="dapA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00665">
        <property type="entry name" value="DHDPS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00666">
        <property type="entry name" value="DHDPS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 9" id="KW-0220">Diaminopimelate biosynthesis</keyword>
    <keyword evidence="9 10 11" id="KW-0456">Lyase</keyword>
    <keyword evidence="7 9" id="KW-0457">Lysine biosynthesis</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 9" id="KW-0704">Schiff base</keyword>
    <feature type="active site" description="Proton donor/acceptor" evidence="9">
        <location>
            <position position="135"/>
        </location>
    </feature>
    <feature type="active site" description="Schiff-base intermediate with substrate" evidence="9">
        <location>
            <position position="163"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="47"/>
        </location>
        <ligand>
            <name>pyruvate</name>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="205"/>
        </location>
        <ligand>
            <name>pyruvate</name>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
        </ligand>
    </feature>
    <feature type="site" description="Part of a proton relay during catalysis" evidence="9">
        <location>
            <position position="46"/>
        </location>
    </feature>
    <feature type="site" description="Part of a proton relay during catalysis" evidence="9">
        <location>
            <position position="109"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00003294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022915"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRNR" id="PIRNR001365"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ABF07954.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="294" mass="31107" checksum="08E513191EDB8088" modified="2006-05-30" version="1">MTQITGSIVAIVTPMHEDGSLDFPALRALIDWHAAEGTDGIVIVGTTGESPTVNVDEHCELIRVAVEQSAGRVPIIAGTGGNSTSEAIELTAFAKKVGANASLQVVPYYNKPTQEGMYRHFRTIAEAVDLPVVLYNVPGRTVADMNNDTILRLAQVPGIVGVKEATGNIDRAAQLINGAPEGFAIYSGDDPTAVALILLGGHGNISVTANVAPRKMHEMCVAALKGDVVTARRLHMELIDLNRAMFVEANPIPVKWALQQMGKMEGGIRLPLTPLAEGQHETVRRALAAAGLLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="69" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LPJ3</accession>
    <name>Q1LPJ3_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">CP_ATPgrasp_1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_1047</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07933.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515839.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1047"/>
    <dbReference type="EnsemblBacteria" id="ABF07933">
        <property type="protein sequence ID" value="ABF07933"/>
        <property type="gene ID" value="Rmet_1047"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1047"/>
    <dbReference type="eggNOG" id="COG2308">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017048_2_0_4"/>
    <dbReference type="OMA" id="VLTPGMY"/>
    <dbReference type="OrthoDB" id="517836at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007302">
        <property type="entry name" value="CP_ATPgrasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016450">
        <property type="entry name" value="UCP005522"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04174">
        <property type="entry name" value="CP_ATPgrasp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005522">
        <property type="entry name" value="UCP005522"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CP_ATPgrasp_1" evidence="1">
        <location>
            <begin position="92"/>
            <end position="423"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF04174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF07933.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="491" mass="54875" checksum="D7648EDF83B8C308" modified="2006-05-30" version="1">MAARFFDEMLEGREDGATEAIEAGRALPSGAVRPHYRNFAEWLGRQSAATMSNKRAEADLIFRRVGITFAVYGDKDESNSGTERTIPFDVIPRIFPASEWGTLERGLRQRVAALNRFIHDIYHGQDIIRAGVIPPDQIYNNAQYRPEMLGIDVPRDIYAHVAGIDVVRAGEGEFYVLEDNLRVPSGVSYMLENRKMMMRLFPDLFARNRVAPVAHYPDLLLDMLRSVSPQNIADPTVVVLTPGMYNSAYFEHAFLAQQMGVELVEGNDLFVEDDHLYMRTTQGPQRIDVIYRRVDDDFLDPLVFRHDSALGAAGLLSVYRAGNVTISNAIGTGVADDKSIYPYVPDMIRFYLGEEPILSNVPTYMCRRPDDLQYVLDHMQDLVVKETHGAGGYGMLVGPAATRAEIDQFREVVKSRPEQYIAQPTLALSTCPTYVDAGIAPRHIDLRPFVLSGKEVRMVPGGLTRVALREGSLVVNSSQGGGTKDTWVLER</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LPK7</accession>
    <name>Q1LPK7_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Rick_17kDa_Anti domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Rmet_1033</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07919.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515825.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1033"/>
    <dbReference type="EnsemblBacteria" id="ABF07919">
        <property type="protein sequence ID" value="ABF07919"/>
        <property type="gene ID" value="Rmet_1033"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1033"/>
    <dbReference type="eggNOG" id="COG3133">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_090265_1_0_4"/>
    <dbReference type="OMA" id="YHEAPPF"/>
    <dbReference type="OrthoDB" id="2074114at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019867">
        <property type="term" value="C:outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008816">
        <property type="entry name" value="Gly_zipper_2TM_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05433">
        <property type="entry name" value="Rick_17kDa_Anti"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="20"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="domain" description="Rick_17kDa_Anti" evidence="3">
        <location>
            <begin position="146"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="50"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="50"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="91"/>
            <end position="126"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF05433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF07919.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="234" mass="24014" checksum="0EE62CA2FB9095FD" modified="2006-05-30" version="1">MSGPETLPSLPTQRRLNPLIGAAAVSIVVASLAAVAAITGVLPISKANQGGTEPTAAQYNAQPATQAETQTPAPGSTTRPPAYDRQYAQNAMPQAPAQQQQQQQQQQQQQQQPSQTEPNTAARASHTAGHVTGVQAVESEKPTSGLGAVGGAVVGGLLGNQIGSGNGRILGTVAGAAAGGFGGNYAEKKLNTNKSYRVSVQMDDGSRRTFTYQNPPGVEVGQRVHLERGVLVTG</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-12-02" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LPT7</accession>
    <name>Q1LPT7_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Putative branched-chain amino acid permease protein (ABC superfamily)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Rmet_0953</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07839.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0953"/>
    <dbReference type="EnsemblBacteria" id="ABF07839">
        <property type="protein sequence ID" value="ABF07839"/>
        <property type="gene ID" value="Rmet_0953"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0953"/>
    <dbReference type="eggNOG" id="COG0559">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027416_1_0_4"/>
    <dbReference type="OMA" id="FGPTNRE"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001851">
        <property type="entry name" value="ABC_transp_permease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017779">
        <property type="entry name" value="ABC_UrtB_bac"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02653">
        <property type="entry name" value="BPD_transp_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03409">
        <property type="entry name" value="urea_trans_UrtB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193407" evidence="7">
        <location>
            <begin position="27"/>
            <end position="545"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="252"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="286"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="311"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="343"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="385"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="479"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="509"/>
            <end position="528"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ABF07839.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="545" mass="57908" checksum="5A9B8B7EA20DEA71" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LQ40</accession>
    <name>Q1LQ40_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Heme chaperone HemW</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">hemN</name>
        <name evidence="5" type="ordered locus">Rmet_0850</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Probably acts as a heme chaperone, transferring heme to an unknown acceptor. Binds one molecule of heme per monomer, possibly covalently. Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the anaerobic coproporphyrinogen-III oxidase family. HemW subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07736.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008646062.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0850"/>
    <dbReference type="EnsemblBacteria" id="ABF07736">
        <property type="protein sequence ID" value="ABF07736"/>
        <property type="gene ID" value="Rmet_0850"/>
    </dbReference>
    <dbReference type="GeneID" id="60825334"/>
    <dbReference type="KEGG" id="rme:Rmet_0850"/>
    <dbReference type="eggNOG" id="COG0635">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027579_2_1_4"/>
    <dbReference type="OMA" id="HIPWCVR"/>
    <dbReference type="OrthoDB" id="1130951at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004109">
        <property type="term" value="F:coproporphyrinogen oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006779">
        <property type="term" value="P:porphyrin-containing compound biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034505">
        <property type="entry name" value="Coproporphyrinogen-III_oxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaB/NifB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010723">
        <property type="entry name" value="HemN_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004559">
        <property type="entry name" value="HemW-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13932">
        <property type="entry name" value="PTHR13932"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06969">
        <property type="entry name" value="HemN_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00562">
        <property type="entry name" value="HemN-like__clustered_with_heat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00539">
        <property type="entry name" value="hemN_rel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="2" id="KW-0143">Chaperone</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2" id="KW-0349">Heme</keyword>
    <keyword evidence="2" id="KW-0408">Iron</keyword>
    <keyword evidence="2" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <feature type="domain" description="Radical SAM core" evidence="4">
        <location>
            <begin position="35"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006100"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU364116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABF07736.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="419" mass="46104" checksum="EC59D9F8F5791CF6" modified="2006-05-30" version="1">MIPIVPVGGGTPATSSPLSPADNQKLWLKPGQIALPGSPPLSLYVHIPWCVRKCPYCDFNSHAAPGKPDSHEIPEDAYLDALRADLEQSLPLVWGRPVHTVFIGGGTPSLLSAAGMDRLLSDIRALLPLDADAEITMEANPGTFEAERFASYRASGINRLSIGIQSFNDAHLKALGRIHGEREARAAIDIALKHFDNVNLDLMYALPGQTLDECARDLDAALSYGTTHLSLYHLTLEPNTLFAKFPPALPDEDTAYEMQDIVEARTAQAGYRHYETSAYAKPHREARHNLNYWRFGDYLGIGAGAHGKLSFPNRILRQMRHKHPATYMEQALAGNAVQEAREVGADELPFEFMLNALRLTDGVPASSFHDYTGLPLHMIGKQLAAAEKKGLLDADPTVIKPTELGRRFLNDLQEMFLKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LQB7</accession>
    <name>Q1LQB7_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Phosphonates transport system permease protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">phnE</name>
        <name evidence="6" type="ordered locus">Rmet_0773</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cell membrane</location>
            <topology evidence="4">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the binding-protein-dependent transport system permease family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07659.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0773"/>
    <dbReference type="EnsemblBacteria" id="ABF07659">
        <property type="protein sequence ID" value="ABF07659"/>
        <property type="gene ID" value="Rmet_0773"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0773"/>
    <dbReference type="eggNOG" id="COG3639">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_064254_0_2_4"/>
    <dbReference type="OMA" id="RFAIIGR"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005887">
        <property type="term" value="C:integral component of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015416">
        <property type="term" value="F:ABC-type phosphonate transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005769">
        <property type="entry name" value="PhnE/PtxC"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="SSF161098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01097">
        <property type="entry name" value="PhnE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2 4" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="46"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="108"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="153"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="212"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="239"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="263"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="5">
        <location>
            <begin position="102"/>
            <end position="284"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABF07659.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="292" mass="31871" checksum="1459D8EB54C9A1AD" modified="2006-05-30" version="1">MAPLGSPSALHFPLQLYLFRLPMTATAKPGLPPTPQSTVRPPRNSLSVMLIWAIVLATLAMSWQGADMRPMDLFRDSGNMAKFAADFFPPDFRDWHTYLDEMLVTVQIALWGTALAVVMAVPFGLLCSANIVPPWIYQPARRLMDACRAINEMVFAMLFIVAVGLGPFAGVLALWIHTMGVLAKLFAEAVEAIDPRPVEGVRATGAGPIEEIVYGVIPQVLPLWLSFALYRFESNVRSASVVGIVGAGGIGTVLWEIIRSFQYAQTCAVMIIIIVFVTGLDIVSAQIRKVLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LQU2</accession>
    <name>Q1LQU2_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Ser/Thr protein phosphatase family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_0598</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07484.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515457.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0598"/>
    <dbReference type="EnsemblBacteria" id="ABF07484">
        <property type="protein sequence ID" value="ABF07484"/>
        <property type="gene ID" value="Rmet_0598"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0598"/>
    <dbReference type="eggNOG" id="COG1409">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_056914_0_0_4"/>
    <dbReference type="OMA" id="NGHIHQV"/>
    <dbReference type="OrthoDB" id="1242748at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP_ApaH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006311">
        <property type="entry name" value="TAT_signal"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="SSF56300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51318">
        <property type="entry name" value="TAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Metallophos" evidence="1">
        <location>
            <begin position="38"/>
            <end position="226"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF07484.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="296" mass="32591" checksum="EA53D27E787C4FF5" modified="2006-05-30" version="1">MHGNWSRRDILRLAGAAGGAVVASALPGWAAGRDEDFVFVQLSDSHWGFEGPPNPDARGTLPKAVAAVNALPSQPDFIMFTGDLTHSTDDPAERRRRMTEVRDIIAPLRAGAVHLMPGEHDASLDAGEAFRELFGPTHYTFDHKGLHAIVLDNVSDPAGMVGEAQRKWLADDLAKQSRDARIVVFTHRPLFDLYPQWDWATRDGAQVIDILMPYRNVTVFYGHIHQEHHHMTGHIAHHAARSLMFPLPAAGSQPKRLPIPWDAAHPYQGLGWRQVTATPSPDGFRLGEVSVNGSKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="106" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LQY4</accession>
    <name>Q1LQY4_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Sulfur carrier protein FdhD</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3 4" type="primary">fdhD</name>
        <name evidence="4" type="ordered locus">Rmet_0556</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Required for formate dehydrogenase (FDH) activity. Acts as a sulfur carrier protein that transfers sulfur from IscS to the molybdenum cofactor prior to its insertion into FDH.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the FdhD family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07442.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515418.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0556"/>
    <dbReference type="EnsemblBacteria" id="ABF07442">
        <property type="protein sequence ID" value="ABF07442"/>
        <property type="gene ID" value="Rmet_0556"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0556"/>
    <dbReference type="eggNOG" id="COG1526">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_056887_2_0_4"/>
    <dbReference type="OMA" id="ECTELVE"/>
    <dbReference type="OrthoDB" id="948173at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097163">
        <property type="term" value="F:sulfur carrier activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016783">
        <property type="term" value="F:sulfurtransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006777">
        <property type="term" value="P:Mo-molybdopterin cofactor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00187">
        <property type="entry name" value="FdhD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016193">
        <property type="entry name" value="Cytidine_deaminase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003786">
        <property type="entry name" value="FdhD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30592">
        <property type="entry name" value="PTHR30592"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02634">
        <property type="entry name" value="FdhD-NarQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015626">
        <property type="entry name" value="FdhD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53927">
        <property type="entry name" value="SSF53927"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00129">
        <property type="entry name" value="fdhD_narQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 3" id="KW-0501">Molybdenum cofactor biosynthesis</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Cysteine persulfide intermediate" evidence="3">
        <location>
            <position position="124"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF07442.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="281" mass="29483" checksum="C44BB326217FD058" modified="2006-05-30" version="1">MKRDMPDENGLGEAGVLPVQHTRAVTRWRQGAASAEIDHLAEEMPVAFEYNGISHAVMLATPADLEDFAIGFSLTEGVVDSARDIYGIEVEAAENGVTVKLEIASAAFVTLKSRRRALAGRTGCGLCGTESLEEVMRAPRKVHSTAVFDPVVFDDAFAALHKRQALLRDTGATHAAAWLRADGDVSLVREDVGRHNALDKLAGALARGGEDVSHGAVIVTSRASYEMVLKTASIGAGILAAVSGSTGLAARLAESAGVTLAGFVRGGTLVAYSHPQRLKLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LQZ4</accession>
    <name>Q1LQZ4_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">D-beta-hydroxybutyrate dehydrogenase</fullName>
            <ecNumber evidence="2">1.1.1.30</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">bdhA</name>
        <name evidence="2" type="ordered locus">Rmet_0546</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.30" evidence="2"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07432.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515408.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0546"/>
    <dbReference type="EnsemblBacteria" id="ABF07432">
        <property type="protein sequence ID" value="ABF07432"/>
        <property type="gene ID" value="Rmet_0546"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0546"/>
    <dbReference type="eggNOG" id="COG1028">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010194_1_0_4"/>
    <dbReference type="OMA" id="FPADKWQ"/>
    <dbReference type="OrthoDB" id="1094766at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003858">
        <property type="term" value="F:3-hydroxybutyrate dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011294">
        <property type="entry name" value="3-OHbutyrate_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01963">
        <property type="entry name" value="PHB_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00061">
        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU000363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF07432.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="258" mass="26786" checksum="AEEA778F213527CE" modified="2006-05-30" version="1">MVNLQGKTALVTGSTSGIGLGIAEALGRAGANIVLNGFGDVDAALATIAATGAQSAHHPADMRKPDEIEAMIAMARERFGTVDVLVNNAGIQHVAQVEDFPAQKWDDILAINLTSSFHTTRHVIPAMRARNWGRIVNIASVHGLVGSAGKSAYVAAKHGLVGLTKVTALETAGTGITCNAICPGFVLTPLVAAQIDAIAKRDGISMEAARARLLSDKQPSGQFVTPEQLGNLVLMLCSPFGDQVRGAAWAMDGGWTAQ</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2007-06-12" modified="2022-08-03" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LR05</accession>
    <name>LPXK_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Tetraacyldisaccharide 4'-kinase</fullName>
            <ecNumber evidence="1">2.7.1.130</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Lipid A 4'-kinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">lpxK</name>
        <name type="ordered locus">Rmet_0535</name>
    </gene>
    <organism>
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Transfers the gamma-phosphate of ATP to the 4'-position of a tetraacyldisaccharide 1-phosphate intermediate (termed DS-1-P) to form tetraacyldisaccharide 1,4'-bis-phosphate (lipid IVA).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + {2-N,3-O-bis[(3R)-3-hydroxytetradecanoyl]-beta-D-glucosaminyl}-(1-&gt;6)-{2-N,3-O-bis[(3R)-3-hydroxytetradecanoyl]-alpha-D-glucosaminyl phosphate} = ADP + {2-N,3-O-bis[(3R)-3-hydroxytetradecanoyl]-4-O-phospho-beta-D-glucosaminyl}-(1-&gt;6)-{2-N,3-O-bis[(3R)-3-hydroxytetradecanoyl]-alpha-D-glucosaminyl phosphate}.</text>
            <dbReference type="EC" id="2.7.1.130"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Glycolipid biosynthesis; lipid IV(A) biosynthesis; lipid IV(A) from (3R)-3-hydroxytetradecanoyl-[acyl-carrier-protein] and UDP-N-acetyl-alpha-D-glucosamine: step 6/6.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the LpxK family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.130" evidence="1"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07421.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515396.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q1LR05"/>
    <dbReference type="SMR" id="Q1LR05"/>
    <dbReference type="STRING" id="266264.Rmet_0535"/>
    <dbReference type="EnsemblBacteria" id="ABF07421">
        <property type="protein sequence ID" value="ABF07421"/>
        <property type="gene ID" value="Rmet_0535"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0535"/>
    <dbReference type="eggNOG" id="COG1663">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038816_2_0_4"/>
    <dbReference type="OMA" id="MDDGFQN"/>
    <dbReference type="OrthoDB" id="1382484at2"/>
    <dbReference type="UniPathway" id="UPA00359">
        <property type="reaction ID" value="UER00482"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009029">
        <property type="term" value="F:tetraacyldisaccharide 4'-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009245">
        <property type="term" value="P:lipid A biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00409">
        <property type="entry name" value="LpxK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003758">
        <property type="entry name" value="LpxK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42724">
        <property type="entry name" value="PTHR42724"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02606">
        <property type="entry name" value="LpxK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00682">
        <property type="entry name" value="lpxK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0418">Kinase</keyword>
    <keyword id="KW-0441">Lipid A biosynthesis</keyword>
    <keyword id="KW-0444">Lipid biosynthesis</keyword>
    <keyword id="KW-0443">Lipid metabolism</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000291230" description="Tetraacyldisaccharide 4'-kinase">
        <location>
            <begin position="1"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="351"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="65"/>
            <end position="72"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="372" mass="40905" checksum="AC311D0376C7446C" modified="2006-05-30" version="1">MAAPQHTLADFVTDQWQRRSWFTWLMWPLSLLFGLIARIRRYGYQQGWFKSTRLPMPVIVVGNVTVGGTGKTPAVIALAHALAEAGLRPGVVSRGYGVKLNHPRRVKPTSKAADVGDEPLLIARAADVPVWVFPDRALCTQAMLVSHPGVNVLLLDDGLQHYKLQRDFEIVMFDTRMGGNGMMLPAGPLREPLTRPRDATLINDPNFRATPDKPDVYGMRLELDEAWQLNDPTMSCDVSKFADKRVLAAAGIGHPERFFASLRQAGLSPATLPLPDHYDFVQDPFADNPAALEADVILITEKDAVKCERFDDPRIWVVPTTPVIDAGLIDKIRRVVQARNPAIATPVTVGQSTATGMADGLDKEHQDGQPAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LR62</accession>
    <name>Q1LR62_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Cyclic pyranopterin monophosphate synthase</fullName>
            <ecNumber evidence="3">4.6.1.17</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Rmet_0478</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(8S)-3',8-cyclo-7,8-dihydroguanosine 5'-triphosphate = cyclic pyranopterin phosphate + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:49580"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:59648"/>
            <dbReference type="ChEBI" id="CHEBI:131766"/>
            <dbReference type="EC" id="4.6.1.17"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Cofactor biosynthesis; molybdopterin biosynthesis.</text>
    </comment>
    <dbReference type="EC" id="4.6.1.17" evidence="3"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07364.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0478"/>
    <dbReference type="EnsemblBacteria" id="ABF07364">
        <property type="protein sequence ID" value="ABF07364"/>
        <property type="gene ID" value="Rmet_0478"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0478"/>
    <dbReference type="eggNOG" id="COG0315">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_208571_0_0_4"/>
    <dbReference type="OMA" id="YINQRTN"/>
    <dbReference type="UniPathway" id="UPA00344"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061799">
        <property type="term" value="F:cyclic pyranopterin monophosphate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006777">
        <property type="term" value="P:Mo-molybdopterin cofactor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.640">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036522">
        <property type="entry name" value="MoaC_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002820">
        <property type="entry name" value="Mopterin_CF_biosynth-C_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01967">
        <property type="entry name" value="MoaC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55040">
        <property type="entry name" value="SSF55040"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0501">Molybdenum cofactor biosynthesis</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MoaC" evidence="5">
        <location>
            <begin position="21"/>
            <end position="52"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001637"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005046"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012575"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABF07364.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="62" mass="6663" checksum="E68E8551617B5D35" modified="2006-05-30" version="1">MEKPSEVATSTHPKSLIYKDVQVALLTIYDMCKAVDRGMVMGNVKLLEKHGGKSGDWVAGGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="69" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LRI2</accession>
    <name>Q1LRI2_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">NTP_transf_3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_0358</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07244.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515237.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0358"/>
    <dbReference type="EnsemblBacteria" id="ABF07244">
        <property type="protein sequence ID" value="ABF07244"/>
        <property type="gene ID" value="Rmet_0358"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0358"/>
    <dbReference type="eggNOG" id="COG2068">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_061980_4_1_4"/>
    <dbReference type="OMA" id="LFGAAHW"/>
    <dbReference type="OrthoDB" id="1432587at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025877">
        <property type="entry name" value="MobA-like_NTP_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12804">
        <property type="entry name" value="NTP_transf_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="SSF53448"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="NTP_transf_3" evidence="2">
        <location>
            <begin position="14"/>
            <end position="175"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12804"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF07244.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="198" mass="21000" checksum="C257B4A6E479F616" modified="2006-05-30" version="1">MTKPVLSQSDLPIGILLAAGFGRRFDPAGARNKLLEKLPDGRTVAWRSARTLAAALPGSIAVVRPGNPELSEELRRGGCKVVESADAEAGMGAALRAGVTACADARGWVVALADMPWLSMELVRAVALTITSRDTIAAPWRDGQRGHPVGFGAAWREELLKLDGDEGARALLKSQPVTRILTDDDGPFRDVDVPGDLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="69" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LRL6</accession>
    <name>Q1LRL6_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Putative integrase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_0324</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07210.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0324"/>
    <dbReference type="EnsemblBacteria" id="ABF07210">
        <property type="protein sequence ID" value="ABF07210"/>
        <property type="gene ID" value="Rmet_0324"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0324"/>
    <dbReference type="eggNOG" id="COG4688">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023352_0_0_4"/>
    <dbReference type="OMA" id="NTIAHYG"/>
    <dbReference type="OrthoDB" id="77366at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.443.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013762">
        <property type="entry name" value="Integrase-like_cat_sf"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF07210.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="667" mass="74656" checksum="3BCBC87120806ACD" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="56" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LRM4</accession>
    <name>Q1LRM4_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_0316</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07202.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515202.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0316"/>
    <dbReference type="EnsemblBacteria" id="ABF07202">
        <property type="protein sequence ID" value="ABF07202"/>
        <property type="gene ID" value="Rmet_0316"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0316"/>
    <dbReference type="eggNOG" id="ENOG5033K04">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1056814_0_0_4"/>
    <dbReference type="OMA" id="GTHSENC"/>
    <dbReference type="OrthoDB" id="1924550at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF07202.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="288" mass="32522" checksum="790A3F9EAE2819CE" modified="2006-05-30" version="1">MRAGICKLTGQHGIFLKSHIIPAALTRKDKSAPFIEAGNGRPPKRTWNSWFDSELVIQRGEKILTEFDTWAIANMRRAKLVWSGWGESTVLEPSMDFSATHQLLEEHDNHLGVHSIQGIDTARLRLFLLSLLWRAAVTKRPEFEEIKLPAGDEQQLRRMLLEGTAEPADFYPITLTQLSTLGPHHNQTPIYRDYHFGPDAERGLPPAKTYRFYFDGLIAIVFLPGYSPDLYEGISAIAVGGKPDRLDVVTVPFEHSFQHENMGKVVLEAHTNWPEKSERLNMAGGNRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LRV4</accession>
    <name>Q1LRV4_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Neutral amino-acid efflux system</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">rhtB</name>
        <name evidence="6" type="ordered locus">Rmet_0236</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07122.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515135.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0236"/>
    <dbReference type="EnsemblBacteria" id="ABF07122">
        <property type="protein sequence ID" value="ABF07122"/>
        <property type="gene ID" value="Rmet_0236"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0236"/>
    <dbReference type="eggNOG" id="COG1280">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_079569_2_1_4"/>
    <dbReference type="OMA" id="PQFINPH"/>
    <dbReference type="OrthoDB" id="1440181at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006865">
        <property type="term" value="P:amino acid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001123">
        <property type="entry name" value="LeuE-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30086">
        <property type="entry name" value="PTHR30086"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01810">
        <property type="entry name" value="LysE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006324">
        <property type="entry name" value="LeuE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="6"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="41"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="122"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="149"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="194"/>
            <end position="210"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABF07122.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="214" mass="23479" checksum="925CEED620B55841" modified="2006-05-30" version="1">MRWEVWLAYFAACWVIAVSPGSGAVLSMSHGLSYGLKKTSTTILGLQTGLVIILLIAGGGLGALLLASEEAFLVVKTIGAMYLIYLGIQQWRAKVETNPQNSEDKTVRVTIMSRRRRFATGLLTNVTNPKGIIFMVAVLPQFIDPTHALGLQLAILAATMCFVDLIVMHGYALLASRMQGLFRNARAVRWQNRFFGSVLVAVGTALFFVRRHHA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="102" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LRW1</accession>
    <name>Q1LRW1_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Histidine kinase</fullName>
            <ecNumber evidence="3">2.7.13.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">dctB</name>
        <name evidence="12" type="ordered locus">Rmet_0229</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.</text>
            <dbReference type="EC" id="2.7.13.3"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.7.13.3" evidence="3"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07115.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515129.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0229"/>
    <dbReference type="EnsemblBacteria" id="ABF07115">
        <property type="protein sequence ID" value="ABF07115"/>
        <property type="gene ID" value="Rmet_0229"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0229"/>
    <dbReference type="eggNOG" id="COG4191">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_94_2_4"/>
    <dbReference type="OMA" id="WRFRATR"/>
    <dbReference type="OrthoDB" id="1755994at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00082">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033479">
        <property type="entry name" value="dCache_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003661">
        <property type="entry name" value="HisK_dim/P"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036097">
        <property type="entry name" value="HisK_dim/P_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029151">
        <property type="entry name" value="Sensor-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004358">
        <property type="entry name" value="Sig_transdc_His_kin-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017055">
        <property type="entry name" value="Sig_transdc_His_kinase_DctB"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02743">
        <property type="entry name" value="dCache_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00512">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036431">
        <property type="entry name" value="STHK_DctB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00344">
        <property type="entry name" value="BCTRLSENSOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00388">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103190">
        <property type="entry name" value="SSF103190"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47384">
        <property type="entry name" value="SSF47384"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="SSF55874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="12" id="KW-0418">Kinase</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="28"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="351"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="11">
        <location>
            <begin position="447"/>
            <end position="663"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="376"/>
            <end position="438"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ABF07115.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="668" mass="71868" checksum="6C3B1301A583B132" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-07-22" modified="2022-05-25" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q58AD3</accession>
    <name>COPK_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName>Copper resistance protein K</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">copK</name>
        <name type="ordered locus">Rmet_6108</name>
    </gene>
    <organism>
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid">
        <name>pMOL30</name>
    </geneLocation>
    <reference key="1">
        <citation type="submission" date="2004-11" db="EMBL/GenBank/DDBJ databases">
            <title>Sequence and features of the Ralstonia metallidurans CH34 heavy metals plasmids pMOL28 and pMOL30.</title>
            <authorList>
                <person name="Monchy S."/>
                <person name="van der Lelie D."/>
                <person name="Vallaeys T."/>
                <person name="Taghavi S."/>
                <person name="Benotmane M."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="Lapidus A."/>
                <person name="Mergeay M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <reference key="3">
        <citation type="journal article" date="2006" name="Microbiology" volume="152" first="1765" last="1776">
            <title>Transcriptomic and proteomic analyses of the pMOL30-encoded copper resistance in Cupriavidus metallidurans strain CH34.</title>
            <authorList>
                <person name="Monchy S."/>
                <person name="Benotmane M.A."/>
                <person name="Wattiez R."/>
                <person name="van Aelst S."/>
                <person name="Auquier V."/>
                <person name="Borremans B."/>
                <person name="Mergeay M."/>
                <person name="Taghavi S."/>
                <person name="van der Lelie D."/>
                <person name="Vallaeys T."/>
            </authorList>
            <dbReference type="PubMed" id="16735739"/>
            <dbReference type="DOI" id="10.1099/mic.0.28593-0"/>
        </citation>
        <scope>PROTEIN SEQUENCE OF 21-38</scope>
        <scope>IDENTIFICATION BY MASS SPECTROMETRY</scope>
        <scope>SUBCELLULAR LOCATION</scope>
        <scope>INDUCTION</scope>
    </reference>
    <reference key="4">
        <citation type="journal article" date="2005" name="Acta Crystallogr. F Struct. Biol. Commun." volume="61" first="825" last="827">
            <title>Overexpression, purification, crystallization and crystallographic analysis of CopK of Cupriavidus metallidurans.</title>
            <authorList>
                <person name="Tricot C."/>
                <person name="van Aelst S."/>
                <person name="Wattiez R."/>
                <person name="Mergeay M."/>
                <person name="Stalon V."/>
                <person name="Wouters J."/>
            </authorList>
            <dbReference type="PubMed" id="16511169"/>
            <dbReference type="DOI" id="10.1107/S174430910502316X"/>
        </citation>
        <scope>PARTIAL PROTEIN SEQUENCE</scope>
        <scope>IDENTIFICATION BY MASS SPECTROMETRY</scope>
        <scope>CRYSTALLIZATION</scope>
    </reference>
    <reference key="5">
        <citation type="journal article" date="2008" name="J. Mol. Biol." volume="380" first="386" last="403">
            <title>Molecular structure and metal-binding properties of the periplasmic CopK protein expressed in Cupriavidus metallidurans CH34 during copper challenge.</title>
            <authorList>
                <person name="Bersch B."/>
                <person name="Favier A."/>
                <person name="Schanda P."/>
                <person name="van Aelst S."/>
                <person name="Vallaeys T."/>
                <person name="Coves J."/>
                <person name="Mergeay M."/>
                <person name="Wattiez R."/>
            </authorList>
            <dbReference type="PubMed" id="18533181"/>
            <dbReference type="DOI" id="10.1016/j.jmb.2008.05.017"/>
        </citation>
        <scope>STRUCTURE BY NMR OF 21-94</scope>
        <scope>FUNCTION</scope>
        <scope>SUBUNIT</scope>
        <scope>INDUCTION</scope>
        <scope>SUBCELLULAR LOCATION</scope>
        <scope>MASS SPECTROMETRY</scope>
    </reference>
    <comment type="function">
        <text evidence="2">Involved in resistance to copper. Can bind up to 2 copper ions. Has higher affinity for Cu(+) than for Cu(2+).</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Monomer in the copper-bound form. Homodimer as apoprotein. Dissociates into monomers upon copper binding.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 2">Periplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="induction">
        <text evidence="1 2">By Cu(2+). Maximally expressed 30 minutes after induction with 0.4 mM copper.</text>
    </comment>
    <comment type="mass spectrometry" mass="8279.58" error="0.06" method="Electrospray" evidence="2"/>
    <dbReference type="EMBL" id="X71400">
        <property type="protein sequence ID" value="CAI11334.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP000354">
        <property type="protein sequence ID" value="ABF12967.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008652571.1">
        <property type="nucleotide sequence ID" value="NC_007971.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_145685.1">
        <property type="nucleotide sequence ID" value="NC_006466.1"/>
    </dbReference>
    <dbReference type="PDB" id="2K0Q">
        <property type="method" value="NMR"/>
        <property type="chains" value="A=21-94"/>
    </dbReference>
    <dbReference type="PDB" id="2KM0">
        <property type="method" value="NMR"/>
        <property type="chains" value="A=21-94"/>
    </dbReference>
    <dbReference type="PDB" id="2LEL">
        <property type="method" value="NMR"/>
        <property type="chains" value="A=21-94"/>
    </dbReference>
    <dbReference type="PDB" id="3DSO">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="1.55 A"/>
        <property type="chains" value="A=21-94"/>
    </dbReference>
    <dbReference type="PDB" id="3DSP">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.20 A"/>
        <property type="chains" value="A=21-94"/>
    </dbReference>
    <dbReference type="PDB" id="3N7D">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.15 A"/>
        <property type="chains" value="A/B=21-94"/>
    </dbReference>
    <dbReference type="PDB" id="3N7E">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.30 A"/>
        <property type="chains" value="A/B=21-94"/>
    </dbReference>
    <dbReference type="PDBsum" id="2K0Q"/>
    <dbReference type="PDBsum" id="2KM0"/>
    <dbReference type="PDBsum" id="2LEL"/>
    <dbReference type="PDBsum" id="3DSO"/>
    <dbReference type="PDBsum" id="3DSP"/>
    <dbReference type="PDBsum" id="3N7D"/>
    <dbReference type="PDBsum" id="3N7E"/>
    <dbReference type="AlphaFoldDB" id="Q58AD3"/>
    <dbReference type="BMRB" id="Q58AD3"/>
    <dbReference type="SMR" id="Q58AD3"/>
    <dbReference type="EnsemblBacteria" id="ABF12967">
        <property type="protein sequence ID" value="ABF12967"/>
        <property type="gene ID" value="Rmet_6108"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6108"/>
    <dbReference type="HOGENOM" id="CLU_185099_0_0_4"/>
    <dbReference type="OMA" id="DGQKIMM"/>
    <dbReference type="OrthoDB" id="2058115at2"/>
    <dbReference type="EvolutionaryTrace" id="Q58AD3"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid pMOL30"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042597">
        <property type="term" value="C:periplasmic space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021604">
        <property type="entry name" value="CopK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038644">
        <property type="entry name" value="CopK_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11525">
        <property type="entry name" value="CopK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0186">Copper</keyword>
    <keyword id="KW-0903">Direct protein sequencing</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0574">Periplasm</keyword>
    <keyword id="KW-0614">Plasmid</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000344219" description="Copper resistance protein K">
        <location>
            <begin position="21"/>
            <end position="94"/>
        </location>
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            <end position="25"/>
        </location>
    </feature>
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        <location>
            <begin position="26"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="strand" evidence="5">
        <location>
            <begin position="37"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="strand" evidence="5">
        <location>
            <begin position="46"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="turn" evidence="4">
        <location>
            <begin position="51"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="strand" evidence="5">
        <location>
            <begin position="64"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="strand" evidence="5">
        <location>
            <begin position="71"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="strand" evidence="5">
        <location>
            <begin position="78"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="84"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="90"/>
            <end position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000269" key="1">
        <source>
            <dbReference type="PubMed" id="16735739"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="2">
        <source>
            <dbReference type="PubMed" id="18533181"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="3">
        <source>
            <dbReference type="PDB" id="2K0Q"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="4">
        <source>
            <dbReference type="PDB" id="2LEL"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="5">
        <source>
            <dbReference type="PDB" id="3DSO"/>
        </source>
    </evidence>
    <sequence length="94" mass="10269" checksum="0714E0E168DFFFE4" modified="2005-04-26" version="1" precursor="true">MKQKLMVGAFIAAVSLSAAAVDMSNVVKTYDLQDGSKVHVFKDGKMGMENKFGKSMNMPEGKVMETRDGTKIIMKGNEIFRLDEALRKGHSEGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-04-26" modified="2021-09-29" version="66" xmlns="http://uniprot.org/uniprot">
    <accession>Q58AQ1</accession>
    <name>Q58AQ1_CUPMC</name>
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        </submittedName>
    </protein>
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        <name evidence="2" type="ordered locus">Rmet_6013</name>
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            <taxon>Betaproteobacteria</taxon>
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    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid pMOL30</plasmid>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
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    </dbReference>
    <dbReference type="RefSeq" id="YP_145564.1">
        <property type="nucleotide sequence ID" value="NC_006466.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF12872">
        <property type="protein sequence ID" value="ABF12872"/>
        <property type="gene ID" value="Rmet_6013"/>
    </dbReference>
    <dbReference type="GeneID" id="60824562"/>
    <dbReference type="KEGG" id="rme:Rmet_6013"/>
    <dbReference type="HOGENOM" id="CLU_1814231_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid pMOL30"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="118"/>
            <end position="142"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF12872.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="142" mass="15253" checksum="7B031C918AD4FC50" modified="2005-04-26" version="1">MLNAISNETTAGLELAVALELFCVTLDWQPGDDEQGDYSVSVWAVDEDDAIRQVAEEMADSGEKSFETEAERVAYIQCVIDGAGQYAAEHVKNRLLSDLDNLLKNDRPEALKAIKAILDGADPAGSTTPAADDAKRSDPPAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-01-04" modified="2022-01-19" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>Q5NV39</accession>
    <name>Q5NV39_CUPMC</name>
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        <submittedName>
            <fullName evidence="4">Conjugal transfer protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">pilS</name>
        <name evidence="4" type="ordered locus">Rmet_6289</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
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        <lineage>
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        <name>pMOL28</name>
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    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
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                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
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                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
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            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
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        </source>
    </reference>
    <dbReference type="EMBL" id="CP000355">
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_007972.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_161651.1">
        <property type="nucleotide sequence ID" value="NC_006525.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF13148">
        <property type="protein sequence ID" value="ABF13148"/>
        <property type="gene ID" value="Rmet_6289"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6289"/>
    <dbReference type="HOGENOM" id="CLU_111917_0_0_4"/>
    <dbReference type="OMA" id="LDEYGFY"/>
    <dbReference type="OrthoDB" id="2139011at2"/>
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        <property type="component" value="Plasmid pMOL28"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045584">
        <property type="entry name" value="Pilin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014911">
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    </dbReference>
    <dbReference type="Pfam" id="PF08805">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SSF54523"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
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    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
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        <location>
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            <begin position="1"/>
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        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="ABF13148.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="210" mass="22500" checksum="41773455625A3FF4" modified="2005-01-04" version="1">MDNNLQTNAAARETTAGENPVPLNRLSRAEQWKRIRSNPRLRGQHGGILDEYGFYLLLVAFSLVAILVLFSGNSTDSQVQQFTTELNKVMGKVKTNYRGRYATVTVASVVDNGTFKDLTTMTVDSGTVTVQPGGGTLTIVPGKLLTANDSVQYTIPNQPDAACTQIASAFQSSAGKIVVNGTTIKDVGGNVNMTQVKCSSDSNTIDLFMS</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-03-23" modified="2020-10-07" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>D3DXQ2</accession>
    <name>D3DXQ2_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_6457</name>
    </gene>
    <organism evidence="1 2">
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        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
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        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ADC45072.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADC45072">
        <property type="protein sequence ID" value="ADC45072"/>
        <property type="gene ID" value="Rmet_6457"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6457"/>
    <dbReference type="HOGENOM" id="CLU_3047194_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADC45072.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="54" mass="5807" checksum="6B720FB4272859FD" modified="2010-03-23" version="1">MAEPAEDGGVDAGADMAVGSKPDMRQLFAEIVHNDRPQIRIGCAKMHNLTLSLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-03-23" modified="2020-10-07" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>D3DXY9</accession>
    <name>D3DXY9_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_6552</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ADC45159.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADC45159">
        <property type="protein sequence ID" value="ADC45159"/>
        <property type="gene ID" value="Rmet_6552"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6552"/>
    <dbReference type="HOGENOM" id="CLU_3047198_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADC45159.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="54" mass="5894" checksum="1E09C29DE1DA42C9" modified="2010-03-23" version="1">MASTGDGKEFGQSFDNAKDDGFQGECNIHAESGEGGAKSLRKTRRYGRGHEHYC</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-03-23" modified="2021-06-02" version="63" xmlns="http://uniprot.org/uniprot">
    <accession>D3DY77</accession>
    <name>D3DY77_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Transcriptional regulator (GntR-family)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Rmet_6647</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="6">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="6">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ADC45247.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518594.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_6647"/>
    <dbReference type="EnsemblBacteria" id="ADC45247">
        <property type="protein sequence ID" value="ADC45247"/>
        <property type="gene ID" value="Rmet_6647"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6647"/>
    <dbReference type="eggNOG" id="COG1802">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017584_5_1_4"/>
    <dbReference type="OrthoDB" id="1911275at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07377">
        <property type="entry name" value="WHTH_GntR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.530">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011711">
        <property type="entry name" value="GntR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008920">
        <property type="entry name" value="TF_FadR/GntR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000524">
        <property type="entry name" value="Tscrpt_reg_HTH_GntR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07729">
        <property type="entry name" value="FCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00392">
        <property type="entry name" value="GntR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00035">
        <property type="entry name" value="HTHGNTR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00895">
        <property type="entry name" value="FCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00345">
        <property type="entry name" value="HTH_GNTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48008">
        <property type="entry name" value="SSF48008"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50949">
        <property type="entry name" value="HTH_GNTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0614">Plasmid</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH gntR-type" evidence="4">
        <location>
            <begin position="6"/>
            <end position="73"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ADC45247.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="227" mass="24731" checksum="6B049A68640FE99B" modified="2010-03-23" version="1">MSEEKKSRTETIRSEIEQEILSGHLPSGTQIDEQQLIDRFGVSRTPAREALIQLASAGLVTLVPRQGAIVSSITLRDYVALSEILVQLESLAARLASRRMTPAERDALATAYEACAAAQGDAEAYRAANDEFHGVLYEGSRNDILAKQIRTMRARMRGMRDYRFEHPARVRASLAEHKAVLDAIKVGDEDGAAEAMVRHISAGGNVYADMIAAMPDDGAAVGRGRRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-03-23" modified="2020-10-07" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>D3DYG6</accession>
    <name>D3DYG6_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_6757</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ADC45336.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_6757"/>
    <dbReference type="EnsemblBacteria" id="ADC45336">
        <property type="protein sequence ID" value="ADC45336"/>
        <property type="gene ID" value="Rmet_6757"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6757"/>
    <dbReference type="HOGENOM" id="CLU_2685193_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADC45336.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="74" mass="7817" checksum="5DDD096C59D6220C" modified="2010-03-23" version="1">MPKSTPHAFAIMKFRMRAEVQGEAEPGASAARGAIVLVFLAAGMNAGAMRLRGLSHVAYAVMNGKGAEESDWIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LA77</accession>
    <name>Q1LA77_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Histone-like bacterial DNA-binding protein (Bph2)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Rmet_6090</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4 5">
        <name>pMOL30</name>
    </geneLocation>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="5">Plasmid pMOL30</plasmid>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the bacterial histone-like protein family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000354">
        <property type="protein sequence ID" value="ABF12949.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011514853.1">
        <property type="nucleotide sequence ID" value="NC_007971.2"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF12949">
        <property type="protein sequence ID" value="ABF12949"/>
        <property type="gene ID" value="Rmet_6090"/>
    </dbReference>
    <dbReference type="GeneID" id="60825030"/>
    <dbReference type="KEGG" id="rme:Rmet_6090"/>
    <dbReference type="HOGENOM" id="CLU_105066_0_0_4"/>
    <dbReference type="OMA" id="HHQRIRN"/>
    <dbReference type="OrthoDB" id="1847472at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid pMOL30"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030527">
        <property type="term" value="F:structural constituent of chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.520.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000119">
        <property type="entry name" value="Hist_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010992">
        <property type="entry name" value="IHF-like_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33175">
        <property type="entry name" value="PTHR33175"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00216">
        <property type="entry name" value="Bac_DNA_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00411">
        <property type="entry name" value="BHL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47729">
        <property type="entry name" value="SSF47729"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0614">Plasmid</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU003939"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF12949.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="147" mass="15338" checksum="BE1589794BF30C4B" modified="2006-05-30" version="1">MATKAKTTAKAAAKPAAKPAAKKAAVKTPAKAAPKAAAAAPVVKPLKETFNKSSLLAHLIAQTELDKKTVQTVLAHLENTIVSAIHKKGAGEFTLPGLFKVAAIQVPATKKRFGKNPFTGVDQWFAAKPASVKVKVRPLKKLKDAAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-10-07" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LAC6</accession>
    <name>Q1LAC6_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_6041</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="1 2">
        <name>pMOL30</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid pMOL30</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000354">
        <property type="protein sequence ID" value="ABF12900.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF12900">
        <property type="protein sequence ID" value="ABF12900"/>
        <property type="gene ID" value="Rmet_6041"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6041"/>
    <dbReference type="HOGENOM" id="CLU_1037752_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid pMOL30"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF12900.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="268" mass="29968" checksum="D2B7DB92F2EBCA75" modified="2006-05-30" version="1">MLRSSKVGRAISDSWRQSCAAGSFRRAMGVRCFNCIEFVFAARHGIAHVCGTDQRRLGIEIATMTATIETPLAAVGKAISGIKAKHPEPDAGGRVPNAQIVEFHERQVDAWRAQAAEASLLAQHKSESGESELALVLSEWSERTMRAADERARYLTSAEYQEWLAGRQEWLAEATVDTVAADNSKRACAFGKGVRVVIAEDIDRRFWRYRSGQTGTVRYVLDDGGVEVLLDGYKREKLSNWWRRIWRGEGANLVPWRGVFKASELRVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="95" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LAN2</accession>
    <name>Q1LAN2_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Methyl-accepting chemotaxis sensory transducer</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">Rmet_5935</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="12">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="12">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the methyl-accepting chemotaxis (MCP) protein family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12794.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5935"/>
    <dbReference type="EnsemblBacteria" id="ABF12794">
        <property type="protein sequence ID" value="ABF12794"/>
        <property type="gene ID" value="Rmet_5935"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5935"/>
    <dbReference type="eggNOG" id="COG0840">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_107_16_4"/>
    <dbReference type="OMA" id="EYIWIND"/>
    <dbReference type="OrthoDB" id="429006at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004888">
        <property type="term" value="F:transmembrane signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006935">
        <property type="term" value="P:chemotaxis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004090">
        <property type="entry name" value="Chemotax_Me-accpt_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003660">
        <property type="entry name" value="HAMP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004089">
        <property type="entry name" value="MCPsignal_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033480">
        <property type="entry name" value="sCache_2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00015">
        <property type="entry name" value="MCPsignal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17200">
        <property type="entry name" value="sCache_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00260">
        <property type="entry name" value="CHEMTRNSDUCR"/>
    </dbReference>
    <dbReference type="SMART" id="SM01049">
        <property type="entry name" value="Cache_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00283">
        <property type="entry name" value="MA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50111">
        <property type="entry name" value="CHEMOTAXIS_TRANSDUC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50885">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-0614">Plasmid</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="190"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="domain" description="HAMP" evidence="10">
        <location>
            <begin position="227"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="domain" description="Methyl-accepting transducer" evidence="9">
        <location>
            <begin position="273"/>
            <end position="502"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00029447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ABF12794.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="520" mass="55007" checksum="7631F4A2506D2415" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LAQ9</accession>
    <name>Q1LAQ9_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Acyl-CoA dehydrogenase-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Rmet_5908</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="9">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="9">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the acyl-CoA dehydrogenase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12767.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518115.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5908"/>
    <dbReference type="EnsemblBacteria" id="ABF12767">
        <property type="protein sequence ID" value="ABF12767"/>
        <property type="gene ID" value="Rmet_5908"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5908"/>
    <dbReference type="eggNOG" id="COG1960">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018204_0_2_4"/>
    <dbReference type="OMA" id="LYREAPM"/>
    <dbReference type="OrthoDB" id="760677at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003995">
        <property type="term" value="F:acyl-CoA dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.540.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.110.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006089">
        <property type="entry name" value="Acyl-CoA_DH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006091">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046373">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036250">
        <property type="entry name" value="AcylCo_DH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009075">
        <property type="entry name" value="AcylCo_DH/oxidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013786">
        <property type="entry name" value="AcylCoA_DH/ox_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037069">
        <property type="entry name" value="AcylCoA_DH/ox_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009100">
        <property type="entry name" value="AcylCoA_DH/oxidase_NM_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00441">
        <property type="entry name" value="Acyl-CoA_dh_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02770">
        <property type="entry name" value="Acyl-CoA_dh_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02771">
        <property type="entry name" value="Acyl-CoA_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47203">
        <property type="entry name" value="SSF47203"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56645">
        <property type="entry name" value="SSF56645"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00072">
        <property type="entry name" value="ACYL_COA_DH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00073">
        <property type="entry name" value="ACYL_COA_DH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0274">FAD</keyword>
    <keyword evidence="3" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="8" id="KW-0614">Plasmid</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Acyl-CoA_dh_N" evidence="7">
        <location>
            <begin position="10"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA_dh_M" evidence="6">
        <location>
            <begin position="121"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA_dh_1" evidence="5">
        <location>
            <begin position="230"/>
            <end position="383"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF02770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF02771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ABF12767.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="385" mass="41464" checksum="5C61AFEE290C1015" modified="2006-05-30" version="1">MALDAESFSLLRASVQRFIDDRLKPAEDTLEEIDDVPADIVADMKEMGLFGISIPESYGGIGLSMSQECDVVYDLGHTAFAFRSVFGTNVGIGSQGILMDGTEEQKQTYLPKIASGELIISFALTEPNAGSDAASLQTRADLDGDHYVINGTKRFITNAPRAGAFTLMARTGGQGASGISSFIVPADTPGISLGKPDKKMGQRGTKTCDVVLENVRVPAANIIGGVPGVGFKTAMKVLDRGRLHISALACGMAHRLITDAVAYAKERKQFGKPIGDFQLIQGMLADSQAELYAGLSMVRDCAQRYDAKPAGKSDPEVSMLASCTKMFCTEMVGRVADRAVQIHGGAGYIAEYKAERFYRDVRLLRLYEGTTQIQQLIIAKQLLRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-10-07" version="54" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LAS9</accession>
    <name>Q1LAS9_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_5888</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12747.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF12747">
        <property type="protein sequence ID" value="ABF12747"/>
        <property type="gene ID" value="Rmet_5888"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5888"/>
    <dbReference type="HOGENOM" id="CLU_601128_0_0_4"/>
    <dbReference type="OMA" id="FDGAWTY"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004192973" evidence="1">
        <location>
            <begin position="24"/>
            <end position="461"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF12747.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="461" mass="45757" checksum="02CCAA1001594F6D" modified="2006-05-30" version="1">MRTRTWTTWLACAAIAMLMVACGGGGGSSGSGSAPSGGAGSGGSAGSGGSGAVATSYTLVLNQTSGKVAVGDTMPLTASVVDNLGNDVTGSTTFAWSTINSSIAVVANASVPGGAVVTGVGLGIANISVVATVTGANNATTVLPQASTTITVVAPGSRTYSLALPYPSLSMTDGQTLRVTASLIDSDGADRSASATGWTWRSTTSAVQAAGVANVGVLLGVNGGSTVALSVVSVQVTAPDGSTLTGAFPVSVFPTGVSSFRLVLSQNGSQINALNVLNGFPQTYLSQVVRSDGNDSTPDFTGLWTFTTTSPSLLVLPDSSTRLTTVSTGRPNGASPLQSILSEMASSLTLSAKPRATLFVTEQPTWALVYNGPQPLVVNRLAGATVAVQLMHRGVDEGITGCGTSWAWSTTSSNIVVAQSVLAPNSASILPTAAGPFSVTATCTAGVEAMPLSITIPGTAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LAW1</accession>
    <name>Q1LAW1_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Glycosyl transferase, group 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">wcaI</name>
        <name evidence="2" type="ordered locus">Rmet_5856</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12715.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518063.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5856"/>
    <dbReference type="CAZy" id="GT4">
        <property type="family name" value="Glycosyltransferase Family 4"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF12715">
        <property type="protein sequence ID" value="ABF12715"/>
        <property type="gene ID" value="Rmet_5856"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5856"/>
    <dbReference type="eggNOG" id="COG0438">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009583_11_5_4"/>
    <dbReference type="OMA" id="WMAREGH"/>
    <dbReference type="OrthoDB" id="1591999at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028098">
        <property type="entry name" value="Glyco_trans_4-like_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13579">
        <property type="entry name" value="Glyco_trans_4_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glyco_trans_4-like_N" evidence="1">
        <location>
            <begin position="15"/>
            <end position="202"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13579"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF12715.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="420" mass="45759" checksum="A9C4CBF5A7C44474" modified="2006-05-30" version="1">MKILLYCLNYAPELTGIGKYTGEQAEWLAARGHQVRVITAPPYYPAWKIDRAYRGLRYRREMRHAVDVMRAPLWVPERPSGVKRLLHLASFAVTSLGWLARQVAWRPDVVFTVEPPLFCSPATLLFARLTGAKAWLHIQDYEVDAAFKLGLLKGGLPQRMASTFERFIMRRYDHVSSISHTMVALARQKGVDATRVSMLPNWVDLAALARRGDADFRARLGIPANAVVALYSGTIGTKQGIEILADAARHLCSRDDLHFIICGAGAGVPALRTQCEGLARVHFLPLQSAEDFPALLDCADIHLMPQRADAADLVLPSKLTNMLASGKPVVATAAPDTELGKLVIQFGSLVEPGNSVAMADAIAALADDPPRRQALGSAGKAWAAAHLDRDAVLAEFERTLLRLRAPRGVADQAAADLAGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LAY0</accession>
    <name>Q1LAY0_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Acyl-CoA dehydrogenase-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_5837</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12696.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518046.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5837"/>
    <dbReference type="EnsemblBacteria" id="ABF12696">
        <property type="protein sequence ID" value="ABF12696"/>
        <property type="gene ID" value="Rmet_5837"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5837"/>
    <dbReference type="eggNOG" id="COG1960">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018204_3_2_4"/>
    <dbReference type="OMA" id="CASTAMI"/>
    <dbReference type="OrthoDB" id="760677at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016627">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-CH group of donors"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.540.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.110.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046373">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036250">
        <property type="entry name" value="AcylCo_DH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013786">
        <property type="entry name" value="AcylCoA_DH/ox_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037069">
        <property type="entry name" value="AcylCoA_DH/ox_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009100">
        <property type="entry name" value="AcylCoA_DH/oxidase_NM_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02771">
        <property type="entry name" value="Acyl-CoA_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47203">
        <property type="entry name" value="SSF47203"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56645">
        <property type="entry name" value="SSF56645"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Acyl-CoA_dh_N" evidence="1">
        <location>
            <begin position="27"/>
            <end position="128"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF12696.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="400" mass="41962" checksum="2AB82800FF75E406" modified="2006-05-30" version="1">MNTGAKVTLAHANPDAGGAMASDALLAAAREAAMVAQRFADAVDQEARFPHEAFEVLRDQGLLGAMVPAVLGGSGASLATVAAICKVLGETCASTGMIYAMHQIQVACLLDHGTGSPWHQHLLARIASEQLLLASATSEEAIGGALRSSGCAVNVDGDRFHLLKMAPTISYGAHADGILITARRNADASPSDQVLVCALKSDYTLQGLNAWDTLGMRGTCSNGFRLEATGQVDQVLPVPFGEIADATMTPVSHILWSSLWLGIASDAVSRAKTFFQGQARARPGQLPPSASRVSEAVSLLQMMEGRVDLALAHQRSRHAGASVSAAFALAAEMNGLKTAMSTMALEVVQQALLVCGMAGYKHGTPFSLGRHLRDLWSAPLMINNDRIQTNTANLLLADRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LAZ8</accession>
    <name>Q1LAZ8_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">ATPase involved in plasmid partitioning</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">parA</name>
        <name evidence="2" type="ordered locus">Rmet_5819</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12678.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008646379.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5819"/>
    <dbReference type="EnsemblBacteria" id="ABF12678">
        <property type="protein sequence ID" value="ABF12678"/>
        <property type="gene ID" value="Rmet_5819"/>
    </dbReference>
    <dbReference type="GeneID" id="60823563"/>
    <dbReference type="KEGG" id="rme:Rmet_5819"/>
    <dbReference type="eggNOG" id="COG1192">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037612_5_3_4"/>
    <dbReference type="OMA" id="SPMDLWA"/>
    <dbReference type="OrthoDB" id="1631847at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025669">
        <property type="entry name" value="AAA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13614">
        <property type="entry name" value="AAA_31"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA_31" evidence="1">
        <location>
            <begin position="4"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="domain" description="AAA_31" evidence="1">
        <location>
            <begin position="66"/>
            <end position="126"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF12678.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="218" mass="23165" checksum="6B8D451B61B674DA" modified="2006-05-30" version="1">MAAKIITVFNQKGGCGKTTVSMHIAGTLGLRGARSLLVDMDEQGTATRWAAQASDERPFPASVIGLAPSGGAMHREVRKFVNDYDYILVDCPPAVHSAAPSSALLISDLAIIPVVPSPPDLWAAVAAKTLAQHAQVQNETLQIRVMANMVQRRVSIARQAIEILGDDGDVPLLNAMVGSRSAFRECQAIGCTVHGVPGAREAVQEVDMMVDEILSLIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="60" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LBG5</accession>
    <name>Q1LBG5_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_5652</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12511.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5652"/>
    <dbReference type="DNASU" id="4042516"/>
    <dbReference type="EnsemblBacteria" id="ABF12511">
        <property type="protein sequence ID" value="ABF12511"/>
        <property type="gene ID" value="Rmet_5652"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5652"/>
    <dbReference type="eggNOG" id="ENOG50316R5">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_108885_2_1_4"/>
    <dbReference type="OMA" id="SHMGPGF"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009936">
        <property type="entry name" value="DUF1468"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07331">
        <property type="entry name" value="TctB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="43"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="107"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="130"/>
            <end position="149"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF12511.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="160" mass="16786" checksum="1C42A9DEC1D62CF8" modified="2006-05-30" version="1">MSHAKGFKKDYYGGTLMALVGLAAVMAGLQYHTGSLSHMGPGFFPVAVGALLAFVGVLIAVSARNDEPKEDKPSGHGHSHAAPDLRGTVCIVLGTLAFLLFGKYGGMIPATFAIVFISALGDRSNSIKQAFLLSIAMCVIAAVVFSWALQLQLPMFTWGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LBJ9</accession>
    <name>Q1LBJ9_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Glycosyl transferase (Family 2)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_5618</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12477.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011520023.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5618"/>
    <dbReference type="CAZy" id="GT2">
        <property type="family name" value="Glycosyltransferase Family 2"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF12477">
        <property type="protein sequence ID" value="ABF12477"/>
        <property type="gene ID" value="Rmet_5618"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5618"/>
    <dbReference type="eggNOG" id="COG0463">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023736_4_0_4"/>
    <dbReference type="OMA" id="RAAMGGW"/>
    <dbReference type="OrthoDB" id="1379602at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001173">
        <property type="entry name" value="Glyco_trans_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00535">
        <property type="entry name" value="Glycos_transf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="SSF48452"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="SSF53448"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glyco_trans_2-like" evidence="2">
        <location>
            <begin position="7"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF12477.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="356" mass="40242" checksum="6504AEB17BC3728C" modified="2006-05-30" version="1">MRICLSMIVKNEAAVIERCLRAVRPHIHAWAISDTGSTDGTQDIIRNTLGDLPGELIERPWVDFSTNRNEALALAAKHGDYALLIDADDTIEASELGELTADCYDLLVQFRSLTYSQPHLIRLGHGFFYTGVTHEYIDCNRPYSRDTLKNAIYRIGDDGARRVSGRKFVEDAGILEKALAADPGNTRYQFYLAQSYRDGGLVDKAIAAYANRATMGGWDEEVYYSMLQMAVLKEGGGASPGDIVEAYLEAYNYRPTRAEAPCELARYFRLRQRHVLARDFARVATGIAQPDDRLFMDQTVYDWRARDELSIALYWCNERDESARLCRELLADPRLPREQHDRVTRNLAFAIGDVPG</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="61" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LBM3</accession>
    <name>Q1LBM3_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_5594</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12453.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011520002.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF12453">
        <property type="protein sequence ID" value="ABF12453"/>
        <property type="gene ID" value="Rmet_5594"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5594"/>
    <dbReference type="HOGENOM" id="CLU_1400456_0_0_4"/>
    <dbReference type="OrthoDB" id="1531407at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193284" evidence="2">
        <location>
            <begin position="24"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="134"/>
            <end position="165"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF12453.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="165" mass="17156" checksum="8E5A866027BC02FA" modified="2006-05-30" version="1">MNAKTILTAAALAAAFVTGAAQAAPAAQKAGEVYGYSYRVNDQRSAFTDGARVGKTDPYTDGAKVGKADPFTDGAKVGKFDPYTDGARIVAGLDRTGVSSEPARKVDPYLDGAKVGKFDPYTDGARMVAGLDRTGVSSEPARKVDPYYDGARAGKFDPYTEGANA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-10-07" version="53" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LBP9</accession>
    <name>Q1LBP9_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_5568</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12427.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5568"/>
    <dbReference type="EnsemblBacteria" id="ABF12427">
        <property type="protein sequence ID" value="ABF12427"/>
        <property type="gene ID" value="Rmet_5568"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5568"/>
    <dbReference type="eggNOG" id="ENOG5030X5A">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1276746_0_0_4"/>
    <dbReference type="OrthoDB" id="2130004at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF12427.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="216" mass="23823" checksum="9C425902BF13B600" modified="2006-05-30" version="1">MHVSLNLKRVNNFGDGVLALGQVDFLLRFLSDPNPIAAGLPPYAMALTCPLAVPSIQDLPPDCSAPATIQIERDILDGHHILTVKLSLGELNIVWLTEPDDPDIWMFIDGWRQFGVVPIVLGVEEAKEERYRIIMAIPECIPSFGDGRGNQEPSDIHERWASILAFCETHGAAFPLVSEHVPPLPRPDNFKALKYVKSCADIALRIATPSPLCIRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LBZ4</accession>
    <name>Q1LBZ4_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Mandelate racemase/muconate lactonizing enzyme</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_5473</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12332.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519878.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5473"/>
    <dbReference type="EnsemblBacteria" id="ABF12332">
        <property type="protein sequence ID" value="ABF12332"/>
        <property type="gene ID" value="Rmet_5473"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5473"/>
    <dbReference type="eggNOG" id="COG4948">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_707555_0_0_4"/>
    <dbReference type="OMA" id="LGYTHAK"/>
    <dbReference type="OrthoDB" id="743369at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047808">
        <property type="term" value="F:D(-)-tartrate dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.390.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034611">
        <property type="entry name" value="D-tartrate_dehydratase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036849">
        <property type="entry name" value="Enolase-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029017">
        <property type="entry name" value="Enolase-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029065">
        <property type="entry name" value="Enolase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013342">
        <property type="entry name" value="Mandelate_racemase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034593">
        <property type="entry name" value="Mandelate_racemase_DgoD-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48080">
        <property type="entry name" value="PTHR48080"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48080:SF5">
        <property type="entry name" value="PTHR48080:SF5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13378">
        <property type="entry name" value="MR_MLE_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00118">
        <property type="entry name" value="D-tartrate_dehydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00922">
        <property type="entry name" value="MR_MLE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51604">
        <property type="entry name" value="SSF51604"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54826">
        <property type="entry name" value="SSF54826"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MR_MLE" evidence="1">
        <location>
            <begin position="160"/>
            <end position="260"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF12332.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="389" mass="42324" checksum="FE480DAB55DE3C68" modified="2006-05-30" version="1">MRIVNIFHKPLRLQTNIANALVNFSEHTVSLVAMVSDQVRNGRPVVGYAFDSIGRYDQQGILGGRMIPRLLKTEPETLQDDDGRLSPAKVLRCAMQNEKPGGHGDRAHAAAALELAAWDLVAKLDDVPAAVAIARHCGREADARIGVYAAGGYYYPGEDVVGLRREIERHIAHGFDRFKIKIGGAPLGQDLERVQAAVDAARKIGQGARVAVDANGRFDIEQAVSYGNAMREFPLMWYEEPCDPLDYASNAELASHYKGALATGENLFSSQDVKNLVLFGGMRRDLDIFQMDSGLSYGVTGYLHMLHEMEERGFSRRFAFPHGGQLLALHVVAGLGLGGCEVYPDIFQPLGGFGDDTRVDAGAVALPSLPGFGFEGKAELWQELASLQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="110" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LC31</accession>
    <name>Q1LC31_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">ATP-dependent transcriptional regulator, LuxR family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_5436</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12295.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5436"/>
    <dbReference type="EnsemblBacteria" id="ABF12295">
        <property type="protein sequence ID" value="ABF12295"/>
        <property type="gene ID" value="Rmet_5436"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5436"/>
    <dbReference type="eggNOG" id="COG2909">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006325_2_0_4"/>
    <dbReference type="OMA" id="SDWVSNA"/>
    <dbReference type="OrthoDB" id="1377603at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06170">
        <property type="entry name" value="LuxR_C_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016032">
        <property type="entry name" value="Sig_transdc_resp-reg_C-effctor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041617">
        <property type="entry name" value="TPR_MalT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000792">
        <property type="entry name" value="Tscrpt_reg_LuxR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00196">
        <property type="entry name" value="GerE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17874">
        <property type="entry name" value="TPR_MalT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00038">
        <property type="entry name" value="HTHLUXR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00421">
        <property type="entry name" value="HTH_LUXR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46894">
        <property type="entry name" value="SSF46894"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00622">
        <property type="entry name" value="HTH_LUXR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50043">
        <property type="entry name" value="HTH_LUXR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH luxR-type" evidence="2">
        <location>
            <begin position="836"/>
            <end position="901"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF12295.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="904" mass="99367" checksum="32B07A87685509CF" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LCB1</accession>
    <name>Q1LCB1_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Extra-cytoplasmic solute receptor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">bug</name>
        <name evidence="4" type="ordered locus">Rmet_5356</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="5">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="5">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Periplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the UPF0065 (bug) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12215.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519761.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5356"/>
    <dbReference type="EnsemblBacteria" id="ABF12215">
        <property type="protein sequence ID" value="ABF12215"/>
        <property type="gene ID" value="Rmet_5356"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5356"/>
    <dbReference type="eggNOG" id="COG3181">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045683_0_0_4"/>
    <dbReference type="OMA" id="PNNLAMF"/>
    <dbReference type="OrthoDB" id="447734at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042597">
        <property type="term" value="C:periplasmic space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005064">
        <property type="entry name" value="BUG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042100">
        <property type="entry name" value="Bug_dom1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42928">
        <property type="entry name" value="PTHR42928"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03401">
        <property type="entry name" value="TctC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017082">
        <property type="entry name" value="YflP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-0675">Receptor</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193301" evidence="3">
        <location>
            <begin position="22"/>
            <end position="321"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF12215.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="321" mass="34140" checksum="B5916A4A2E6A3DA4" modified="2006-05-30" version="1">MRRLLLACLIATLGVSGLAHANANNYPRKPVKIVVSFPPGTGSDSSARFLAQKLEARFGQPFVVENRPGANSFIAAQTVAKADPDGYTLFVASNSPMATNVVQFKTLPYDPVRDFAPVARLYYGAMILSVRADSPYKTVADLVAAAKVKPGKLSYGSGSASYQIATELFMKQAGITAVAIPYKGAAPAVADLAGGQVDFAMSDYLAASALSQAGKTRIIAVGGDKRLPLEPNVPTLQELGYKDYYMVNWVALFAPAKTPPAIVKKLSDATVEIISSKEAAEFVGRTSGQIFAGGSEVLRKYQLDEIQRWTKAAEIAGIPKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LCC1</accession>
    <name>Q1LCC1_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Rmet_5346</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="8">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="8">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the UPF0187 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12205.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519750.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF12205">
        <property type="protein sequence ID" value="ABF12205"/>
        <property type="gene ID" value="Rmet_5346"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5346"/>
    <dbReference type="eggNOG" id="COG3781">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029790_4_2_4"/>
    <dbReference type="OMA" id="TDICGAC"/>
    <dbReference type="OrthoDB" id="1348684at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005254">
        <property type="term" value="F:chloride channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021134">
        <property type="entry name" value="Bestrophin/UPF0187"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044669">
        <property type="entry name" value="UPF0187"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33281">
        <property type="entry name" value="PTHR33281"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01062">
        <property type="entry name" value="Bestrophin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0614">Plasmid</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="50"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="218"/>
            <end position="249"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABF12205.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="303" mass="33711" checksum="7657E4E06E7C7EA6" modified="2006-05-30" version="1">MITRPQLHWFRMLFVLRGSVLPAIAPQLLGVTLFSALITAFHGRIWDWKVSLNFVPFSLIGLTLAIFLGFRNSTSYARYWEGRTLWGSVLIESRSLVRQALTLTSDPDAARQLAHRLCVFVHTLRHQLRGTDPAADIAPFVDTATSQRMRDARFKPAIALLIAGEWLGEQLRLGKLSPSLALPMENALSHLGSALGGCERLASTPIPFTYSVIIHRCIYLYCVLLPFGLLDTIGLMTPVIVCFIAYTFFALEALSAEIEEPFGEEPNDLALATMSYMIESTVLEMVGEQPLSPRPVNDGYIQT</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LCZ4</accession>
    <name>Q1LCZ4_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Acetoacetyl-CoA reductase</fullName>
            <ecNumber evidence="3">1.1.1.100</ecNumber>
            <ecNumber evidence="3">1.1.1.36</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">phaB3</name>
        <name evidence="3" type="ordered locus">Rmet_5123</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.100" evidence="3"/>
    <dbReference type="EC" id="1.1.1.36" evidence="3"/>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11982.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519529.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5123"/>
    <dbReference type="EnsemblBacteria" id="ABF11982">
        <property type="protein sequence ID" value="ABF11982"/>
        <property type="gene ID" value="Rmet_5123"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5123"/>
    <dbReference type="eggNOG" id="COG1028">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010194_1_3_4"/>
    <dbReference type="OMA" id="KGAFYCA"/>
    <dbReference type="OrthoDB" id="1601931at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004316">
        <property type="term" value="F:3-oxoacyl-[acyl-carrier-protein] reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018454">
        <property type="term" value="F:acetoacetyl-CoA reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042619">
        <property type="term" value="P:poly-hydroxybutyrate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011283">
        <property type="entry name" value="Acetoacetyl-CoA_reductase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01829">
        <property type="entry name" value="AcAcCoA_reduct"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00061">
        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF11982.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="248" mass="26575" checksum="EF93DB24F0289694" modified="2006-05-30" version="1">MSSGRIAVVTGGMGGLGEAIAERLLSDGFRVVVTHSLGNDHVATWLREHSTQQREFVAYPVDVADFESCQQCAARILSEVGPVDVLINNAGITRDSTLRKMTHEAWRAVMSTDLDSVFNMTKPFCEGMVERRWGRIVNISSINGSKGAFGQTNYAAAKAGIHGFSKSLALELASKGVTVNTVSPGYLDTKMVRAVPQDVLDQHVIPQIPVGRLGQPKEVAALISYLCSDDGAYVTGANLAINGGQHMD</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LD21</accession>
    <name>Q1LD21_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">2-ketoglutarate semialdehyde dehydrogenase / NADP-dependent aldehyde dehydrogenase</fullName>
            <ecNumber evidence="3">1.2.1.26</ecNumber>
            <ecNumber evidence="3">1.2.1.4</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">aldH</name>
        <name evidence="3" type="ordered locus">Rmet_5096</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EC" id="1.2.1.26" evidence="3"/>
    <dbReference type="EC" id="1.2.1.4" evidence="3"/>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11955.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5096"/>
    <dbReference type="EnsemblBacteria" id="ABF11955">
        <property type="protein sequence ID" value="ABF11955"/>
        <property type="gene ID" value="Rmet_5096"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5096"/>
    <dbReference type="eggNOG" id="COG1012">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027555_0_0_4"/>
    <dbReference type="OMA" id="AINCITT"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047533">
        <property type="term" value="F:2,5-dioxovalerate dehydrogenase (NADP+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033721">
        <property type="term" value="F:aldehyde dehydrogenase (NADP+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd07129">
        <property type="entry name" value="ALDH_KGSADH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.309.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.605.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016161">
        <property type="entry name" value="Ald_DH/histidinol_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016163">
        <property type="entry name" value="Ald_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016162">
        <property type="entry name" value="Ald_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015590">
        <property type="entry name" value="Aldehyde_DH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044151">
        <property type="entry name" value="ALDH_KGSADH"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00171">
        <property type="entry name" value="Aldedh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53720">
        <property type="entry name" value="SSF53720"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aldedh" evidence="2">
        <location>
            <begin position="34"/>
            <end position="421"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF11955.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="495" mass="52064" checksum="B04A5A98F9EEADEE" modified="2006-05-30" version="1">MQSFRAPLASPLFRQTPSFPFMHASEQNQVFAIIDQHATRAHEASAAWAHSSPTVRSGVLRALADALDARANELAVMADEETGLGIPRLTGEIARTSFQLRGFADEVVAGVPYVQVDDEAIPGPPPSGRPRLTRVMVPIGPVAVFAASNFPFAFSVLGGDTASALAAGCPVIVKPHGGHPRLSIEVAKIAAEVLRAQGVEEGVFSMLDCDMSRDANIHLVKHPLIAGVAFTGSYQGGVALWRAANEREIPIPFFGELGSINPVVVLPAALHDDPVAPAKVLAASMLQGCGQFCTSPGVIVVVDDQRGREFVSALGDSLAGQATHRMLSEGIRNGFEDAVARIKANANIEVVLEGNEAGAGPAPRLFQTTGAAFIADASLHEEMFGPAAVVVMVPSVDAIPDVLSAVKGSLTVTIWGGDQDNHENREIVRVAQNIGGRVLFSGVPTGVAVTRAQQHGGPWPASTDPKTTSVGYAALQRFLRPVALQDAPEWLLQRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="65" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LD30</accession>
    <name>Q1LD30_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Microcystinase C</fullName>
            <shortName evidence="1">MlrC</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Rmet_5087</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="5">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="5">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in peptidolytic degradation of cyclic heptapeptide hepatotoxin microcystin (MC).</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="1">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase M81 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11946.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519493.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5087"/>
    <dbReference type="EnsemblBacteria" id="ABF11946">
        <property type="protein sequence ID" value="ABF11946"/>
        <property type="gene ID" value="Rmet_5087"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5087"/>
    <dbReference type="eggNOG" id="COG5476">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028172_1_0_4"/>
    <dbReference type="OMA" id="GPMWDPI"/>
    <dbReference type="OrthoDB" id="421877at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008237">
        <property type="term" value="F:metallopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009197">
        <property type="entry name" value="MlrC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010799">
        <property type="entry name" value="MlrC_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015995">
        <property type="entry name" value="MlrC_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07364">
        <property type="entry name" value="DUF1485"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07171">
        <property type="entry name" value="MlrC_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF012702">
        <property type="entry name" value="UCP012702"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="4" id="KW-0614">Plasmid</keyword>
    <keyword evidence="1" id="KW-0645">Protease</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF1485" evidence="3">
        <location>
            <begin position="2"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="domain" description="MlrC_C" evidence="2">
        <location>
            <begin position="297"/>
            <end position="471"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRNR" id="PIRNR012702"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF07364"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF11946.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="482" mass="51651" checksum="AFDEB38F47AE7C97" modified="2006-05-30" version="1">MRVFSASLATETNTFAPMPTGLASFRDRGYFPAGQHPDQMTFFSGPLWAARLRGKERGWTLLEGMVAGAQPSGTTTRHAYETLREELLADLRAALPVDMVLLGLHGAMVADGYDDCEGDLLQRVRAIVGPDVVIGAELDPHCHLSPAMTTHADLLIAFKEYPHTDVLERAIELVDLCAAKVEGKIRPVPAVVDCGMIVTVHTSRQPARGFVDRIQALEGHNGVLSISIAHGFSWGDVPEMGTRVLVYTDGDATAAQTLARRLADELIDMREALDVPYPRIDAALDEALAFDGGPVVLADGADNPGGGAAGDSTFILRRMIERGIVNAAIGPMWDPIAARIAFEAGVGARLPLRIGGKISPLSGEPLDLDCTVKALMPDMVMTGLANAPMQMGDSALVEANGIDIVLITLRNQAMGTDLFTQLGCDLAAKKIIVVKSSQHFYASFAKIARHVIYAGAPGAVTLDLRTLPYRKVHRPKWPLDPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="116" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LD67</accession>
    <name>Q1LD67_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 5 7">Histidine ammonia-lyase</fullName>
            <shortName evidence="5">Histidase</shortName>
            <ecNumber evidence="3 5 7">4.3.1.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5 9" type="primary">hutH</name>
        <name evidence="9" type="ordered locus">Rmet_5047</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="10">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="10">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 5 7">
            <text>L-histidine = NH4(+) + trans-urocanate</text>
            <dbReference type="Rhea" id="RHEA:21232"/>
            <dbReference type="ChEBI" id="CHEBI:17771"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:57595"/>
            <dbReference type="EC" id="4.3.1.3"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 5 7">Amino-acid degradation; L-histidine degradation into L-glutamate; N-formimidoyl-L-glutamate from L-histidine: step 1/3.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5 8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="PTM">
        <text evidence="5">Contains an active site 4-methylidene-imidazol-5-one (MIO), which is formed autocatalytically by cyclization and dehydration of residues Ala-Ser-Gly.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5 6">Belongs to the PAL/histidase family.</text>
    </comment>
    <dbReference type="EC" id="4.3.1.3" evidence="3 5 7"/>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11909.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519460.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5047"/>
    <dbReference type="EnsemblBacteria" id="ABF11909">
        <property type="protein sequence ID" value="ABF11909"/>
        <property type="gene ID" value="Rmet_5047"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5047"/>
    <dbReference type="eggNOG" id="COG2986">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014801_4_0_4"/>
    <dbReference type="OMA" id="MLTRCNS"/>
    <dbReference type="OrthoDB" id="715502at2"/>
    <dbReference type="UniPathway" id="UPA00379">
        <property type="reaction ID" value="UER00549"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004397">
        <property type="term" value="F:histidine ammonia-lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019556">
        <property type="term" value="P:histidine catabolic process to glutamate and formamide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019557">
        <property type="term" value="P:histidine catabolic process to glutamate and formate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00332">
        <property type="entry name" value="PAL-HAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.275.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00229">
        <property type="entry name" value="His_ammonia_lyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001106">
        <property type="entry name" value="Aromatic_Lyase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024083">
        <property type="entry name" value="Fumarase/histidase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005921">
        <property type="entry name" value="HutH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008948">
        <property type="entry name" value="L-Aspartase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022313">
        <property type="entry name" value="Phe/His_NH3-lyase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10362">
        <property type="entry name" value="PTHR10362"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00221">
        <property type="entry name" value="Lyase_aromatic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48557">
        <property type="entry name" value="SSF48557"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01225">
        <property type="entry name" value="hutH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00488">
        <property type="entry name" value="PAL_HISTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 5 7" id="KW-0369">Histidine metabolism</keyword>
    <keyword evidence="5 6 9" id="KW-0456">Lyase</keyword>
    <keyword evidence="9" id="KW-0614">Plasmid</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="modified residue" description="2,3-didehydroalanine (Ser)" evidence="5">
        <location>
            <position position="152"/>
        </location>
    </feature>
    <feature type="cross-link" description="5-imidazolinone (Ala-Gly)" evidence="5">
        <location>
            <begin position="151"/>
            <end position="153"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU004480"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ABF11909.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="518" mass="55113" checksum="9AC27D4444EA9E0A" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="70" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LDB0</accession>
    <name>Q1LDB0_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Antibiotic biosynthesis monooxygenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_5004</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11866.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519416.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5004"/>
    <dbReference type="EnsemblBacteria" id="ABF11866">
        <property type="protein sequence ID" value="ABF11866"/>
        <property type="gene ID" value="Rmet_5004"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5004"/>
    <dbReference type="eggNOG" id="COG1359">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_131496_11_0_4"/>
    <dbReference type="OrthoDB" id="1909149at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007138">
        <property type="entry name" value="ABM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011008">
        <property type="entry name" value="Dimeric_a/b-barrel"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03992">
        <property type="entry name" value="ABM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54909">
        <property type="entry name" value="SSF54909"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51725">
        <property type="entry name" value="ABM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABM" evidence="1">
        <location>
            <begin position="2"/>
            <end position="90"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF11866.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="93" mass="10230" checksum="42C387BBA840F0D2" modified="2006-05-30" version="1">MLAVVAQIKVKPGAEAEFEKVAAELVAASRQEKGCLEYTLWRTAETGLYAFTEKYRDADAVDAHRKSEHFRSIGKAMGAFMDGPPNVLRLSAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="101" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LDB1</accession>
    <name>Q1LDB1_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transcriptional regulator, MarR family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_5003</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11865.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519415.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5003"/>
    <dbReference type="EnsemblBacteria" id="ABF11865">
        <property type="protein sequence ID" value="ABF11865"/>
        <property type="gene ID" value="Rmet_5003"/>
    </dbReference>
    <dbReference type="GeneID" id="60826167"/>
    <dbReference type="KEGG" id="rme:Rmet_5003"/>
    <dbReference type="eggNOG" id="COG1846">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_083287_15_1_4"/>
    <dbReference type="OMA" id="SMTRTVN"/>
    <dbReference type="OrthoDB" id="1744037at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000835">
        <property type="entry name" value="HTH_MarR-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12802">
        <property type="entry name" value="MarR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00347">
        <property type="entry name" value="HTH_MARR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50995">
        <property type="entry name" value="HTH_MARR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH marR-type" evidence="1">
        <location>
            <begin position="13"/>
            <end position="149"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF11865.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="150" mass="16134" checksum="43BA757F1CE8FAF8" modified="2006-05-30" version="1">MSKSPPSASDALAHATAEDLRALIGTLRRRLREEARAGDFTPSQIQVLHLLERDGPATVAGLARALDVRPQSMGETLTVLKAAGAVSGAPDPNDGRQTVLSLTPAFRQKVKASRAAREDWLYRAIQSRFTAAEQQQLATGVELLKRLVAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="97" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LDE6</accession>
    <name>Q1LDE6_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Di-heme cytochrome c peroxidase</fullName>
            <ecNumber evidence="11">1.11.1.5</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">Rmet_4968</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="12">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="12">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
        <text evidence="6">Binds 2 heme groups.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Periplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="PTM">
        <text evidence="6">Binds 2 heme groups per subunit.</text>
    </comment>
    <dbReference type="EC" id="1.11.1.5" evidence="11"/>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11830.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="STRING" id="266264.Rmet_4968"/>
    <dbReference type="PeroxiBase" id="5087">
        <property type="entry name" value="RmDiHCcP"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF11830">
        <property type="protein sequence ID" value="ABF11830"/>
        <property type="gene ID" value="Rmet_4968"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4968"/>
    <dbReference type="eggNOG" id="COG1858">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_034652_1_0_4"/>
    <dbReference type="OMA" id="KGPINAP"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042597">
        <property type="term" value="C:periplasmic space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004130">
        <property type="term" value="F:cytochrome-c peroxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.760.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009056">
        <property type="entry name" value="Cyt_c-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036909">
        <property type="entry name" value="Cyt_c-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004852">
        <property type="entry name" value="Di-haem_cyt_c_peroxidsae"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026259">
        <property type="entry name" value="MauG/Cytc_peroxidase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03150">
        <property type="entry name" value="CCP_MauG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00034">
        <property type="entry name" value="Cytochrom_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000294">
        <property type="entry name" value="Cytochrome-c_peroxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46626">
        <property type="entry name" value="SSF46626"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51007">
        <property type="entry name" value="CYTC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 6 8" id="KW-0349">Heme</keyword>
    <keyword evidence="5 7 8" id="KW-0408">Iron</keyword>
    <keyword evidence="3 7 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-0574">Periplasm</keyword>
    <keyword evidence="11" id="KW-0575">Peroxidase</keyword>
    <keyword evidence="11" id="KW-0614">Plasmid</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193369" evidence="9">
        <location>
            <begin position="32"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="domain" description="Cytochrome c" evidence="10">
        <location>
            <begin position="51"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="domain" description="Cytochrome c" evidence="10">
        <location>
            <begin position="203"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="binding site" description="covalent" evidence="6">
        <location>
            <position position="73"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
            <label>1</label>
        </ligand>
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    <feature type="binding site" description="covalent" evidence="6">
        <location>
            <position position="76"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="7">
        <location>
            <position position="77"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
            <label>1</label>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <feature type="binding site" description="covalent" evidence="6">
        <location>
            <position position="217"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" description="covalent" evidence="6">
        <location>
            <position position="220"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="7">
        <location>
            <position position="221"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
            <label>2</label>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="7">
        <location>
            <position position="292"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
            <label>2</label>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022764"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR000294-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR000294-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ABF11830.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="342" mass="37394" checksum="D6AEB9EA1FE5C2AF" modified="2006-05-30" version="1">MKSSRSRVLSTFSTATFITVLFAATVSSAHAAEPQAEPIQPIAPAKVQHPERVALGEKLWFDPRLSRSGFISCNSCHNLSMGGSDNLKTSIGDKWKQGGINSPTVLNSSLNFVQFWDGRAKDLPEQARGPIANPIEMAFTHELAVDTLRSIPGYVDEFRKVFKSVKLTISEITLAIAAFEETLVTPNARFDKWLKGDKTAINAQELRGYQLFKGSGCVACHNGQNIGGNSFQKMGLVEPYKTGNPSEGRAAVTGKDADRFNFKVPTLRNVALTYPYFHDGEAATLSQAVDTMGRLQLARKFTPEENADIVAFLKTLTGDQPKIVLPMLPPSSDKTPKSKPFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="105" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LDS1</accession>
    <name>Q1LDS1_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Putative ABC transporter, ATP binding component involved in iron transport</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">Rmet_4843</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="11">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="11">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11705.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519268.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4843"/>
    <dbReference type="EnsemblBacteria" id="ABF11705">
        <property type="protein sequence ID" value="ABF11705"/>
        <property type="gene ID" value="Rmet_4843"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4843"/>
    <dbReference type="eggNOG" id="COG3842">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_1_4"/>
    <dbReference type="OMA" id="HITAIHV"/>
    <dbReference type="OrthoDB" id="1200451at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043190">
        <property type="term" value="C:ATP-binding cassette (ABC) transporter complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008995">
        <property type="entry name" value="Mo/tungstate-bd_C_term_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013611">
        <property type="entry name" value="Transp-assoc_OB_typ2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08402">
        <property type="entry name" value="TOBE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50331">
        <property type="entry name" value="SSF50331"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-0614">Plasmid</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-1278">Translocase</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ABC transporter" evidence="9">
        <location>
            <begin position="7"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="40"/>
            <end position="47"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ABF11705.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="352" mass="38347" checksum="34E2808A414A1054" modified="2006-05-30" version="1">MHEPTAIRLRHCAKTFADGTTALQPLDLDIGAGETVVLLGPSGCGKTTTLRIIAGLEQPDAGGEVWFGDTAVTDQPIEQRGVGMVFQNYALFPNMTVAENIAYGLRVRRTDSATRKRRVNDMLAMMHLEPFADRRVDQLSGGQRQRVALARAIAVQPRVLLLDEPLTALDAKLRDALRADINQLLRSLHITAVYVTHDQAEAMALGDRIIVMDKGRIAQAGTPQEIYRSPANAFVADFIGTMNHLPAVAEARRWRVPGGTVPMAAPNDAPVSAQARLMFRPEDVALVQADQAHVEGTVVTALFLGNHTRLLVDVGTSQPMVVETARRDAWQAGERVGLRIDTDHLITLHEAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="103" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LDY6</accession>
    <name>Q1LDY6_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="9">High-affinity branched-chain amino acid transport protein of the ABC transporter, ATP binding</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">livF10</name>
        <name evidence="9" type="ordered locus">Rmet_4778</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="10">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="10">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11640.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519208.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4778"/>
    <dbReference type="EnsemblBacteria" id="ABF11640">
        <property type="protein sequence ID" value="ABF11640"/>
        <property type="gene ID" value="Rmet_4778"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4778"/>
    <dbReference type="eggNOG" id="COG0410">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_2_4"/>
    <dbReference type="OMA" id="ICLVEHD"/>
    <dbReference type="OrthoDB" id="1475841at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="1" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-0614">Plasmid</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-1278">Translocase</keyword>
    <feature type="domain" description="ABC transporter" evidence="8">
        <location>
            <begin position="5"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="37"/>
            <end position="44"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ABF11640.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="233" mass="25382" checksum="DB0DC907D7276AFC" modified="2006-05-30" version="1">MSDLLRLETVSAGYGEATVLEQIDLALGAGDSLALLGRNGVGKTTLLSTLMGFTRLRGGKLVWQGRDLARVPPHRRAQSGIGWVPQERWVFPSLTVEEHLGAVARPGPWDVQRVYRTFPRLGERRRNLGNQLSGGEQQMLAIGRALMVNPALLLLDEPMEGLAPVIVQELQRVIAGLIADAGMAVIVVEQHARLALGMTRQAIVLDRGRVVHRSDSATLLANKALLDRLVSVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-12-02" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LE31</accession>
    <name>Q1LE31_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">5-carboxymethyl-2-hydroxymuconate isomerase</fullName>
            <ecNumber evidence="1">5.3.3.10</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">hpcD</name>
        <name evidence="1" type="ordered locus">Rmet_4733</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EC" id="5.3.3.10" evidence="1"/>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11595.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4733"/>
    <dbReference type="EnsemblBacteria" id="ABF11595">
        <property type="protein sequence ID" value="ABF11595"/>
        <property type="gene ID" value="Rmet_4733"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4733"/>
    <dbReference type="eggNOG" id="COG3232">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_139188_3_0_4"/>
    <dbReference type="OMA" id="ENTRLNC"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008704">
        <property type="term" value="F:5-carboxymethyl-2-hydroxymuconate delta-isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019439">
        <property type="term" value="P:aromatic compound catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00580">
        <property type="entry name" value="CHMI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.429.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004220">
        <property type="entry name" value="5-COMe_2-OHmuconate_Isoase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014347">
        <property type="entry name" value="Tautomerase/MIF_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37950">
        <property type="entry name" value="PTHR37950"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02962">
        <property type="entry name" value="CHMI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55331">
        <property type="entry name" value="SSF55331"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0413">Isomerase</keyword>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF11595.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="144" mass="15727" checksum="75AEFB3E1AA46F04" modified="2006-05-30" version="1">MRARRTGMPYDAGFPLSTGPGSMPHLVIELTENTRLTCSQEELLDEANAALLSSGQFQEPDIKSRCVTLSTYRQGTENVDRAFVHATLQILDGREQAVRKELGQLVCNAISEMVRPGASAQSVQVSVNVVEMERATYSKQIIGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LE38</accession>
    <name>Q1LE38_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Diguanylate cyclase/phosphodiesterase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Rmet_4726</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="6">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="6">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11588.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4726"/>
    <dbReference type="EnsemblBacteria" id="ABF11588">
        <property type="protein sequence ID" value="ABF11588"/>
        <property type="gene ID" value="Rmet_4726"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4726"/>
    <dbReference type="eggNOG" id="COG2199">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG2200">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015702_2_1_4"/>
    <dbReference type="OMA" id="WSELRPD"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="CDD" id="cd01948">
        <property type="entry name" value="EAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.580.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.450">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001633">
        <property type="entry name" value="EAL_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035919">
        <property type="entry name" value="EAL_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000160">
        <property type="entry name" value="GGDEF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029787">
        <property type="entry name" value="Nucleotide_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00571">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00563">
        <property type="entry name" value="EAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00990">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00052">
        <property type="entry name" value="EAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00267">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF141868">
        <property type="entry name" value="SSF141868"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="SSF54631"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55073">
        <property type="entry name" value="SSF55073"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00254">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51371">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50883">
        <property type="entry name" value="EAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50887">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0129">CBS domain</keyword>
    <keyword evidence="5" id="KW-0614">Plasmid</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="EAL" evidence="2">
        <location>
            <begin position="67"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="4">
        <location>
            <begin position="423"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="domain" description="GGDEF" evidence="3">
        <location>
            <begin position="511"/>
            <end position="668"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABF11588.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="676" mass="72710" checksum="EDDFC5FDF8C9864E" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LE51</accession>
    <name>Q1LE51_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Type II secretion system core protein G</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">gspG</name>
        <name evidence="13" type="ordered locus">Rmet_4713</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="14">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="14">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell inner membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the GSP G family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11575.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519146.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4713"/>
    <dbReference type="EnsemblBacteria" id="ABF11575">
        <property type="protein sequence ID" value="ABF11575"/>
        <property type="gene ID" value="Rmet_4713"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4713"/>
    <dbReference type="eggNOG" id="COG2165">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_091705_2_0_4"/>
    <dbReference type="OMA" id="VAPKYFS"/>
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        <property type="entry name" value="N_methyl"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="BCTERIALGSPG"/>
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        <property type="entry name" value="IV_pilin_GFxxxE"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="38"/>
            <end position="58"/>
        </location>
    </feature>
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        <location>
            <begin position="61"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="139"/>
            <end position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00020042"/>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF08334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ABF11575.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="166" mass="18927" checksum="7AD656D076EB108B" modified="2006-05-30" version="1">MRIDTQPKRLAQRRWRWVTRRFPDHRPYRLAVGCPPGFTLLELLVVMVIIGLLAGLVAPRYFEQIGKSNTNVAKAQVDSLSKALDAYRLDVGAYPTTEERLDALVTRPQNAPRWNGPYLQKRVPLDPWDRPYQYRSPGEHGDYDLMSFGKDGQPGGTGENSDITSW</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LE63</accession>
    <name>Q1LE63_CUPMC</name>
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        <submittedName>
            <fullName evidence="4">Multicopper oxidase, type 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Rmet_4701</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
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        <lineage>
            <taxon>Bacteria</taxon>
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            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
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        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="5">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="5">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Periplasm</location>
        </subcellularLocation>
    </comment>
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        <property type="gene ID" value="Rmet_4701"/>
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    <dbReference type="OMA" id="HNEREIT"/>
    <dbReference type="Proteomes" id="UP000002429">
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        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    <proteinExistence type="predicted"/>
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        <location>
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            <end position="19"/>
        </location>
    </feature>
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        <location>
            <begin position="20"/>
            <end position="531"/>
        </location>
    </feature>
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        <location>
            <begin position="73"/>
            <end position="181"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF07732"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF11563.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="531" mass="56079" checksum="9A41E5585AAF9949" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-10-07" version="69" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LEA8</accession>
    <name>Q1LEA8_CUPMC</name>
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        <recommendedName>
            <fullName evidence="1">ABM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_4656</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
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        <property type="protein sequence ID" value="ABF11518.1"/>
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    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4656"/>
    <dbReference type="EnsemblBacteria" id="ABF11518">
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        <property type="gene ID" value="Rmet_4656"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4656"/>
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    <dbReference type="OMA" id="IWVTEVW"/>
    <dbReference type="Proteomes" id="UP000002429">
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    </dbReference>
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    </dbReference>
    <dbReference type="InterPro" id="IPR011008">
        <property type="entry name" value="Dimeric_a/b-barrel"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03992">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51725">
        <property type="entry name" value="ABM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABM" evidence="1">
        <location>
            <begin position="29"/>
            <end position="115"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF11518.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="126" mass="13912" checksum="702B3B71182F15BB" modified="2006-05-30" version="1">MIDEDTTLDHHWLKMTQVELFCDEETRMYGLIGKMKVIPGQREALASILLEGVSGMPGCLSYIVAHDPNDADAIWITEVWDSQDSHRASLSLPSVQQAIVRGKPLIAGFGERFETTPIGGQGLTTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="108" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LED2</accession>
    <name>Q1LED2_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Transcriptional regulator, AraC family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Rmet_4632</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="6">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="6">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
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        <property type="protein sequence ID" value="ABF11494.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519069.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4632"/>
    <dbReference type="EnsemblBacteria" id="ABF11494">
        <property type="protein sequence ID" value="ABF11494"/>
        <property type="gene ID" value="Rmet_4632"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4632"/>
    <dbReference type="eggNOG" id="COG2207">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047522_1_2_4"/>
    <dbReference type="OMA" id="LMVGESW"/>
    <dbReference type="OrthoDB" id="1622875at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032687">
        <property type="entry name" value="AraC-type_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018060">
        <property type="entry name" value="HTH_AraC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020449">
        <property type="entry name" value="Tscrpt_reg_HTH_AraC-type"/>
    </dbReference>
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        <property type="entry name" value="Arabinose_bd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12833">
        <property type="entry name" value="HTH_18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00032">
        <property type="entry name" value="HTHARAC"/>
    </dbReference>
    <dbReference type="SMART" id="SM00342">
        <property type="entry name" value="HTH_ARAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01124">
        <property type="entry name" value="HTH_ARAC_FAMILY_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0614">Plasmid</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH araC/xylS-type" evidence="4">
        <location>
            <begin position="234"/>
            <end position="332"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS01124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABF11494.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="335" mass="37309" checksum="7B11DFD0ED597B4E" modified="2006-05-30" version="1">MSTLIRSALLVKFSEVSRTVGIDADRLIQHVGGDRSCMFSPDLHVPEPWLARMLESSEQAGQGASASIGLLMAETWRMSDFGPISLLLQHQPTLRHALGQLETYRHLLSESVAVHVEEFDDIAIVHQQVITERPNPGRQPVELSVGALQSLMKSMLGGHWKPRSVHFSHAAPASLHVHQRLFGTRVEFGCDFDGITIDRHDLDRPNPLGDVNLARYAKEFLDERPHGKQASTASNVRRAIQLLLPRGRSGIEQVGQQLGMSSRTLQRRLEQEGVEFSTLLGEVRRGLANRYLGDQRYPVSQVATLLGFSEVSTFSRWFAAQFGVSPTRWRAGQPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LEN7</accession>
    <name>Q1LEN7_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Negative transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_4524</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11389.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518982.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4524"/>
    <dbReference type="EnsemblBacteria" id="ABF11389">
        <property type="protein sequence ID" value="ABF11389"/>
        <property type="gene ID" value="Rmet_4524"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4524"/>
    <dbReference type="eggNOG" id="COG2808">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_065853_0_1_4"/>
    <dbReference type="OMA" id="ARANPQW"/>
    <dbReference type="OrthoDB" id="1804797at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.110.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012349">
        <property type="entry name" value="Split_barrel_FMN-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007396">
        <property type="entry name" value="TR_PAI2-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35802">
        <property type="entry name" value="PTHR35802"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04299">
        <property type="entry name" value="FMN_bind_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF010372">
        <property type="entry name" value="PaiB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF11389.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="216" mass="24073" checksum="E8F463EA1F19BA1D" modified="2006-05-30" version="1">MYVPAHFEESRPEALHALMTAYPFGTLVTHGRNGLDANHIPFHLDPSQGPNGMLKAHVARANPVWQDVADGDEVLVIFRAADAYISPNWYPSKHEAHKQVPTWNYMVVHAHGRITIRDDERFVRGVVAHLTRTHEATQPRPWKMSDAPSDFVDTMLKAIVGIEIEITRLEGKLKLSQNKEVRDIRGAGTALAEQGSTTLGEAMLARADAKERGETQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="114" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LFD7</accession>
    <name>Q1LFD7_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">3-isopropylmalate dehydratase large subunit</fullName>
            <ecNumber evidence="12">4.2.1.33</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Alpha-IPM isomerase</fullName>
            <shortName evidence="12">IPMI</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">Isopropylmalate isomerase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12 14" type="primary">leuC</name>
        <name evidence="14" type="ordered locus">Rmet_4273</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="15">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="15">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2 12">Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 12">
            <text>(2R,3S)-3-isopropylmalate = (2S)-2-isopropylmalate</text>
            <dbReference type="Rhea" id="RHEA:32287"/>
            <dbReference type="ChEBI" id="CHEBI:1178"/>
            <dbReference type="ChEBI" id="CHEBI:35121"/>
            <dbReference type="EC" id="4.2.1.33"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="12">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="12">Binds 1 [4Fe-4S] cluster per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3 12">Amino-acid biosynthesis; L-leucine biosynthesis; L-leucine from 3-methyl-2-oxobutanoate: step 2/4.</text>
    </comment>
    <comment type="subunit">
        <text evidence="12">Heterodimer of LeuC and LeuD.</text>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the aconitase/IPM isomerase family. LeuC type 1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.33" evidence="12"/>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11139.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518765.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4273"/>
    <dbReference type="EnsemblBacteria" id="ABF11139">
        <property type="protein sequence ID" value="ABF11139"/>
        <property type="gene ID" value="Rmet_4273"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4273"/>
    <dbReference type="eggNOG" id="COG0065">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006714_3_4_4"/>
    <dbReference type="OMA" id="GIEHCLL"/>
    <dbReference type="OrthoDB" id="749418at2"/>
    <dbReference type="UniPathway" id="UPA00048">
        <property type="reaction ID" value="UER00071"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003861">
        <property type="term" value="F:3-isopropylmalate dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016853">
        <property type="term" value="F:isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009098">
        <property type="term" value="P:leucine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01583">
        <property type="entry name" value="IPMI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.499.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01026">
        <property type="entry name" value="LeuC_type1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004430">
        <property type="entry name" value="3-IsopropMal_deHydase_lsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015931">
        <property type="entry name" value="Acnase/IPM_dHydase_lsu_aba_1/3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001030">
        <property type="entry name" value="Acoase/IPM_deHydtase_lsu_aba"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018136">
        <property type="entry name" value="Aconitase_4Fe-4S_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036008">
        <property type="entry name" value="Aconitase_4Fe-4S_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033941">
        <property type="entry name" value="IPMI_cat"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00330">
        <property type="entry name" value="Aconitase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00415">
        <property type="entry name" value="ACONITASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53732">
        <property type="entry name" value="SSF53732"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00170">
        <property type="entry name" value="leuC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00450">
        <property type="entry name" value="ACONITASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01244">
        <property type="entry name" value="ACONITASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 12" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="6 12" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="11 12" id="KW-0100">Branched-chain amino acid biosynthesis</keyword>
    <keyword evidence="8 12" id="KW-0408">Iron</keyword>
    <keyword evidence="9 12" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="14" id="KW-0413">Isomerase</keyword>
    <keyword evidence="4 12" id="KW-0432">Leucine biosynthesis</keyword>
    <keyword evidence="10 12 14" id="KW-0456">Lyase</keyword>
    <keyword evidence="7 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-0614">Plasmid</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aconitase" evidence="13">
        <location>
            <begin position="12"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="352"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="413"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="416"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ABF11139.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="477" mass="50794" checksum="CB005B7D4BA77175" modified="2006-05-30" version="1">MVQANPPASLLDKLWASHVIHEQSDGPSLIYIDRHLVNEVTSPQAFEGLRLHGREVWRPASVLATADHNVPTTERAAGIADPISRAQVDALERNCAATGIVNFGMNDPRQGILHVVGPERGATLPGMTIVCGDSHTSTHGAFGAFAFGIGTSEVEQVLSAQCLWMKKPRSMLVRVEGELAAGVSAKDIALALIGRIGTAGATGYVIEFAGSTIRGLSMEGRMTVCNMAIEAGARAGMVAVDETTLAYLAHRPQAPQGEAWESASAYWRTLRSDPDAVFDAVVDIGVATIRPHVTWGTSPEMVVAIDERIPDPRQEADPVRRRGMERALTYMGLEPGIKVASIALDKVFIGSCTNARLEDLRAAADVLRGRHVASNIRQALVVPGSGLVKSQAEAEGLDRVFIDAGFEWREPGCSMCLGMNDDRLAPGERCASTSNRNFEGRQGPGGRTHLVSPQMAAAAAVAGHFVDVSTVLQEKSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LFE1</accession>
    <name>Q1LFE1_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">AMP-dependent synthetase and ligase</fullName>
            <ecNumber evidence="3">6.2.1.26</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_4269</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EC" id="6.2.1.26" evidence="3"/>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11135.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518761.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4269"/>
    <dbReference type="EnsemblBacteria" id="ABF11135">
        <property type="protein sequence ID" value="ABF11135"/>
        <property type="gene ID" value="Rmet_4269"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4269"/>
    <dbReference type="eggNOG" id="COG0318">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000022_59_9_4"/>
    <dbReference type="OMA" id="YFNSGDM"/>
    <dbReference type="OrthoDB" id="377638at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008756">
        <property type="term" value="F:o-succinylbenzoate-CoA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025110">
        <property type="entry name" value="AMP-bd_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045851">
        <property type="entry name" value="AMP-bd_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020845">
        <property type="entry name" value="AMP-binding_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_Synth/Lig"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13193">
        <property type="entry name" value="AMP-binding_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00455">
        <property type="entry name" value="AMP_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0436">Ligase</keyword>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AMP-binding" evidence="1">
        <location>
            <begin position="34"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="domain" description="AMP-binding_C" evidence="2">
        <location>
            <begin position="443"/>
            <end position="518"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF11135.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="539" mass="58459" checksum="BA4B0F4BE78E10A9" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LFH0</accession>
    <name>Q1LFH0_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Outer membrane protein (Porin)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">omp</name>
        <name evidence="8" type="ordered locus">Rmet_4239</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="9">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="9">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11106.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4239"/>
    <dbReference type="EnsemblBacteria" id="ABF11106">
        <property type="protein sequence ID" value="ABF11106"/>
        <property type="gene ID" value="Rmet_4239"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4239"/>
    <dbReference type="eggNOG" id="COG3203">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038238_2_0_4"/>
    <dbReference type="OMA" id="NASYMNN"/>
    <dbReference type="OrthoDB" id="1064531at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009279">
        <property type="term" value="C:cell outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046930">
        <property type="term" value="C:pore complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015288">
        <property type="term" value="F:porin activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034220">
        <property type="term" value="P:ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00342">
        <property type="entry name" value="gram_neg_porins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.160.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033900">
        <property type="entry name" value="Gram_neg_porin_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023614">
        <property type="entry name" value="Porin_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001702">
        <property type="entry name" value="Porin_Gram-ve"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002299">
        <property type="entry name" value="Porin_Neis"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13609">
        <property type="entry name" value="Porin_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00182">
        <property type="entry name" value="ECOLNEIPORIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00184">
        <property type="entry name" value="NEISSPPORIN"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0406">Ion transport</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0614">Plasmid</keyword>
    <keyword evidence="5" id="KW-0626">Porin</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1134">Transmembrane beta strand</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Porin_4" evidence="7">
        <location>
            <begin position="36"/>
            <end position="348"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF13609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ABF11106.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="377" mass="40020" checksum="729554C37FA714D8" modified="2006-05-30" version="1">MQRHLTSQSIRNVCLYKPIPNMEEKVKLKRKSLVVATVSAMFAASAQAQSSVTLYGVADAGIEYVSHAGLDGTGSAYRVSSDNTSGSRWGLRGKEDLGGGLSAVFVLESGFSIDTGTMAQGGRLFGRQAFVGISSHLGQITLGRQNNTLFDLFAGLDPLRYATYSLLSQDAQFAGRPDNSVKYTGNFGALTITGLYSAGYDSNIANGTEVPGSPRVGQEMGAGASYTSGNLGMAIAYDQRRGTSVLNASAVERRYVASLIYTTGPFSAVAGYRYLQGGLTPTTGIRSNLYWVGGGYAITPALRLNLGVYYTDRRDSPSDSASYVLQTQYAFSKRTDLYVNASYMNNKGKSNLGVVTSTTVAPGVSQTGIVAGVRHIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LFK4</accession>
    <name>Q1LFK4_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Multifunctional methyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_4205</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11072.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518695.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4205"/>
    <dbReference type="EnsemblBacteria" id="ABF11072">
        <property type="protein sequence ID" value="ABF11072"/>
        <property type="gene ID" value="Rmet_4205"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4205"/>
    <dbReference type="eggNOG" id="COG0684">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_072626_3_2_4"/>
    <dbReference type="OMA" id="VNVPVVC"/>
    <dbReference type="OrthoDB" id="1614890at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047443">
        <property type="term" value="F:4-hydroxy-4-methyl-2-oxoglutarate aldolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd16841">
        <property type="entry name" value="RraA_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014165">
        <property type="entry name" value="LigK_PcmE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005493">
        <property type="entry name" value="RraA/RraA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036704">
        <property type="entry name" value="RraA/RraA-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03737">
        <property type="entry name" value="RraA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89562">
        <property type="entry name" value="SSF89562"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02798">
        <property type="entry name" value="ligK_PcmE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF11072.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="237" mass="24929" checksum="06D98BABD05F8F33" modified="2006-05-30" version="1">MRTVAYRNILRAESRDVEALGKFGVATVHEAQRRTGLMKPYLRPIQSGSAISGCAVTALMHPGDNWMIHVAVEMVQPGDVLVVGLVSDCTDGVFGDLLATSLKARGARGLIIDCGVRDVSTLNVMGFPVWSRAISAQGAVKETVGAVNVPVVCAGALVRPGDVIVADDDGVVVVARPQAADIAAKAAEREALEAGKREKLASGVLGVDMYNMRPGLAEKGLVYLDQTVDWYSGANEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="59" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LFX7</accession>
    <name>Q1LFX7_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_4082</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10949.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008644323.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4082"/>
    <dbReference type="EnsemblBacteria" id="ABF10949">
        <property type="protein sequence ID" value="ABF10949"/>
        <property type="gene ID" value="Rmet_4082"/>
    </dbReference>
    <dbReference type="GeneID" id="60823341"/>
    <dbReference type="KEGG" id="rme:Rmet_4082"/>
    <dbReference type="eggNOG" id="COG4274">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_155227_2_0_4"/>
    <dbReference type="OMA" id="HDMVAVC"/>
    <dbReference type="OrthoDB" id="1774162at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014845">
        <property type="entry name" value="GYD/TTHA1554"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08734">
        <property type="entry name" value="GYD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF10949.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="97" mass="10604" checksum="05FA9679AF958969" modified="2006-05-30" version="1">MTRYIVLASFTDQGIRTVKETTKRAAAVRELGAQLGVQVKDVFWTLGKYDVVLTLEAQDDTVVTAFGLSVGALGNVHTQTLRAFDATEMQAILGKMR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LGE7</accession>
    <name>Q1LGE7_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Putative amino acid (Threonine) efflux protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Rmet_3911</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="7">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="7">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10779.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518426.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3911"/>
    <dbReference type="EnsemblBacteria" id="ABF10779">
        <property type="protein sequence ID" value="ABF10779"/>
        <property type="gene ID" value="Rmet_3911"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3911"/>
    <dbReference type="eggNOG" id="COG1280">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_079569_3_2_4"/>
    <dbReference type="OMA" id="KMGWDCM"/>
    <dbReference type="OrthoDB" id="1927576at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006865">
        <property type="term" value="P:amino acid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001123">
        <property type="entry name" value="LeuE-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30086">
        <property type="entry name" value="PTHR30086"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01810">
        <property type="entry name" value="LysE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006324">
        <property type="entry name" value="LeuE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0614">Plasmid</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="6"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="43"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="71"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="113"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="149"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="187"/>
            <end position="205"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABF10779.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="208" mass="22203" checksum="DD141E85497C14EC" modified="2006-05-30" version="1">MPPFDTLLPFLGIAVLLSLAPGPDNIFVLLLSAMHGTRAGLTVVLGLCTGLLVHTAVVAVGLAALLAASTVAFTVLKIAGALYLVYLAWQAFRAPVGELSSSQMQVKTGKHMYLRGVIMNLTNPKVVIFFLAFLPQFVSPDRGPVAPQIMVLGFVFILATLLVFGSIAWFSGAFGRVLMRSARMQRAVNWFAGSVFLALAGRLALSQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LGH3</accession>
    <name>Q1LGH3_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative Asp/Glu racemase</fullName>
            <ecNumber evidence="2">5.1.99.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_3885</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EC" id="5.1.99.-" evidence="2"/>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10753.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518400.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3885"/>
    <dbReference type="EnsemblBacteria" id="ABF10753">
        <property type="protein sequence ID" value="ABF10753"/>
        <property type="gene ID" value="Rmet_3885"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3885"/>
    <dbReference type="eggNOG" id="COG4126">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_053002_0_1_4"/>
    <dbReference type="OMA" id="FARPPRK"/>
    <dbReference type="OrthoDB" id="1273626at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036361">
        <property type="term" value="F:racemase activity, acting on amino acids and derivatives"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006807">
        <property type="term" value="P:nitrogen compound metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015942">
        <property type="entry name" value="Asp/Glu/hydantoin_racemase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01177">
        <property type="entry name" value="Asp_Glu_race"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0413">Isomerase</keyword>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="222"/>
            <end position="245"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF10753.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="245" mass="25679" checksum="4FB3C1C63810DCA6" modified="2006-05-30" version="1">MNLLIVNPNISESVTDLIHAEAQRTVSPDTRITMATAPFGVAYIETRFEALVGGYATACAAAEHAGQFDGLIVAAFGDPGLAGLKELFDVPVVGMTEAALASACLLGQRFSIIAISHRIQAWYRECVAANGLGDRLASIRSLQEPLRDIGSVQEDHSARLEELALQAVREDGADVIIVAGAPLAGLARSLKGRIPVPVVDGVSSAVRHCESLVALQAGSAREGSFARPPRKPNAGLPPALSRLLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="56" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LGM6</accession>
    <name>Q1LGM6_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_3830</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10700.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518346.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3830"/>
    <dbReference type="EnsemblBacteria" id="ABF10700">
        <property type="protein sequence ID" value="ABF10700"/>
        <property type="gene ID" value="Rmet_3830"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3830"/>
    <dbReference type="eggNOG" id="ENOG50347DD">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1174649_0_0_4"/>
    <dbReference type="OMA" id="VWDLGAP"/>
    <dbReference type="OrthoDB" id="1881135at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF10700.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="236" mass="26441" checksum="88B9615A9A33CA99" modified="2006-05-30" version="1">MRCDPELSLGATDMPADVAAQMPPRGLVIDAMASSVCTLSRGTLLTRAAWNAATALPDFVTHTHRWGPPVSCRQAAGRFPFHWLYVAEDTYTAIWEGRFCEKVERYPGAFRLNEAASDGLLVTMRLKSDLNFLEIGGTTAARLGIYDRLSDPDYQWCRHFGLEMHHVLEALFNRTGAIGIRYPSRRLRNHAALAVHSRHLGRWRESVTIDAARFGDLPIYGELLDDPCRLTEALSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-10-07" version="64" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LGX4</accession>
    <name>Q1LGX4_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_3730</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10602.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3730"/>
    <dbReference type="EnsemblBacteria" id="ABF10602">
        <property type="protein sequence ID" value="ABF10602"/>
        <property type="gene ID" value="Rmet_3730"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3730"/>
    <dbReference type="eggNOG" id="COG1289">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_064722_0_0_4"/>
    <dbReference type="OMA" id="PHGEWAS"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006726">
        <property type="entry name" value="PHBA_efflux_AaeB/fusaric-R"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04632">
        <property type="entry name" value="FUSC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="45"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="116"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="142"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="173"/>
            <end position="195"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF10602.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="362" mass="39622" checksum="127C48CAC46BCB6A" modified="2006-05-30" version="1">MRNPGSALYTRSVHFSVIAMFEALRKTTSALFDPDRRFRQARIFHAVRVALALLVSIALTTGIHIPHGEWATITVLVVIGGLQHHGNIRRRAAERGAGTVIGAFLGLLLILQEAYFHIPVLTWLLLAVICGYCAYHAVGKGGYIALLAAITVVITAGHGNQHVEDALWRTLDVLIGTVIALAFSFALPAYASYSWRFKLAGMLRSCAAVHAKIARGMRDEKERQQDMMKLSASLVQLRSLIPSVAKETGVPTADLEEVQHSARVCISALEMLAAIDPVAAEPTDEERHIRDLLLEMASALESGDESMVQLNLPLPEPHPDDPSIGHSSHTFLTLHLAAEIGHLRDHLAQMARQSRFPYNGTH</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="59" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LGY9</accession>
    <name>Q1LGY9_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_3715</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10587.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518234.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3715"/>
    <dbReference type="EnsemblBacteria" id="ABF10587">
        <property type="protein sequence ID" value="ABF10587"/>
        <property type="gene ID" value="Rmet_3715"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3715"/>
    <dbReference type="HOGENOM" id="CLU_2234306_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193116" evidence="2">
        <location>
            <begin position="25"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="28"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="62"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF10587.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="105" mass="10866" checksum="3A9BB8E290E0DAC8" modified="2006-05-30" version="1">MHRNTYFLAIAAASVALTSLPASAQKNYTDGGDLYGGAHAPVTKDAQAPAKSGKHKNSASRSGKFDPYSEGAQTKSNLTPQKKGAGDAAHAAKFDPYTEGQAAKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LHA6</accession>
    <name>Q1LHA6_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Flavin-containing monooxygenase FMO</fullName>
            <ecNumber evidence="5">1.14.13.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Rmet_3598</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the FAD-binding monooxygenase family.</text>
    </comment>
    <dbReference type="EC" id="1.14.13.-" evidence="5"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10470.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3598"/>
    <dbReference type="EnsemblBacteria" id="ABF10470">
        <property type="protein sequence ID" value="ABF10470"/>
        <property type="gene ID" value="Rmet_3598"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3598"/>
    <dbReference type="eggNOG" id="COG2072">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006937_7_1_4"/>
    <dbReference type="OMA" id="WNTGGCT"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004499">
        <property type="term" value="F:N,N-dimethylaniline monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020946">
        <property type="entry name" value="Flavin_mOase-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00743">
        <property type="entry name" value="FMO-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="SSF51905"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0274">FAD</keyword>
    <keyword evidence="2" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="5" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="4 5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABF10470.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="524" mass="59088" checksum="EE1A403F49EFFD91" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="106" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LHN9</accession>
    <name>Q1LHN9_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transcriptional regulator, AraC-family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_3465</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10337.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517895.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3465"/>
    <dbReference type="EnsemblBacteria" id="ABF10337">
        <property type="protein sequence ID" value="ABF10337"/>
        <property type="gene ID" value="Rmet_3465"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3465"/>
    <dbReference type="eggNOG" id="COG4977">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_59_0_4"/>
    <dbReference type="OMA" id="THWAHCQ"/>
    <dbReference type="OrthoDB" id="871215at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002818">
        <property type="entry name" value="DJ-1/PfpI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018060">
        <property type="entry name" value="HTH_AraC"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01965">
        <property type="entry name" value="DJ-1_PfpI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12833">
        <property type="entry name" value="HTH_18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00342">
        <property type="entry name" value="HTH_ARAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="SSF52317"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01124">
        <property type="entry name" value="HTH_ARAC_FAMILY_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH araC/xylS-type" evidence="1">
        <location>
            <begin position="230"/>
            <end position="328"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS01124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF10337.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="341" mass="37135" checksum="E6BCB4D1F5C7448A" modified="2006-05-30" version="1">MHPAPEPPRVVVIVAPDPAQELDVTGPTAVFGAANKLCGRESPPYDLHVVSVSDDAIVRTESGLRILADAPYHRIVEQVGRPIDTLLISGGSGHARAAEDEQLIGWLRTQATQVRRMGAVCTGAFVLAGTGLLDAQRVTTHWRHAERLAHRHPELDVDADPIWIRAGRFYTSAGVSAGMDLSLALVEEDLGHTVSLDVARDLVLFLRRPGNQAQFSTVLRAQAAQSPELRALQTWIADNLGRDLSVGVLAEQVAMSPRNFARVFVKQVGDTPARYVERLRVEAVRRLLEGGDRRLEAIAHVTGFGNADVMRQAFQRHVQTTPERYRAAFRGEGQAVALAAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-10-07" version="51" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LI07</accession>
    <name>Q1LI07_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_3347</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10219.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3347"/>
    <dbReference type="EnsemblBacteria" id="ABF10219">
        <property type="protein sequence ID" value="ABF10219"/>
        <property type="gene ID" value="Rmet_3347"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3347"/>
    <dbReference type="eggNOG" id="ENOG502ZAAN">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_620643_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029470">
        <property type="entry name" value="PDDEXK_4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14281">
        <property type="entry name" value="PDDEXK_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF10219.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="432" mass="49224" checksum="B0350F2DBFB546BB" modified="2006-05-30" version="1">MGFVEQGQGQLDLLTPRENQHSDLLAWCFNAREGHGQGDAILKDFLVALYGAASDVEPGDRVAGKGLSRDFIRQWTPARVLTTSFATAFCYREYTLPRIDGDGGARRLDLLVVDQANRLLIVVENKAGTRFRTGQLEGYLESVQRTLLTRPAFREFSVVFVALDRDYLVNDDDESGAQDFDRRWVRLNYEWLKPAGKRAEFSVERGNQSAALLLSYCRRQTGWESESMKIVTKLAVDIAVRYPTLVKQFADVAKELGQPESWTPTLMSPDSVEGQLLRFYRQYESAVDSLLDLTPLQRLHTKLAEEFSTLDNDWEVHEYARVRAAYQLPSDQSMPKTDGAWPLYLYIRHINPDTNGTSMFRVMLCWRPWLIPEDARQRVCDALAKVYPAVASTVDRTKTRWLEREECSFDRAVALGAEIVRKIDGELGGARY</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="67" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LI10</accession>
    <name>Q1LI10_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_3344</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10216.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3344"/>
    <dbReference type="EnsemblBacteria" id="ABF10216">
        <property type="protein sequence ID" value="ABF10216"/>
        <property type="gene ID" value="Rmet_3344"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3344"/>
    <dbReference type="eggNOG" id="COG1289">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_392256_0_0_4"/>
    <dbReference type="OMA" id="NRQNRAV"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="45"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="69"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="157"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="311"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="341"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="388"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="417"/>
            <end position="437"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF10216.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="632" mass="66317" checksum="E4C74BA87909ED27" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-12-02" version="67" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LI85</accession>
    <name>Q1LI85_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Type IV pilus assembly protein PilP</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">pilP</name>
        <name evidence="3" type="ordered locus">Rmet_3269</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10141.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3269"/>
    <dbReference type="EnsemblBacteria" id="ABF10141">
        <property type="protein sequence ID" value="ABF10141"/>
        <property type="gene ID" value="Rmet_3269"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3269"/>
    <dbReference type="eggNOG" id="COG3168">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_109321_1_0_4"/>
    <dbReference type="OMA" id="SGCWTER"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007446">
        <property type="entry name" value="PilP"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04351">
        <property type="entry name" value="PilP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016481">
        <property type="entry name" value="Pilus_assembly_PilP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193523" evidence="2">
        <location>
            <begin position="27"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="63"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF10141.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="179" mass="19987" checksum="0933F5B353894A94" modified="2006-05-30" version="1">MSRLTRRPPMALRAVAVAALAVVALAGCGAGEDEHLQQWMAQTRAAKVPPPEPLPEVKPYEPRQYAGTKSPEPFAQTKIGELNKAMSESPEAGRRHEPLEDYPLENFKMLGMLKKNGETYGVVRVDNKIHHVKVGQYLGQNYGRVVRITDQEIVLRELVREGVSEWKEKMTSLKLEASA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LIG3</accession>
    <name>Q1LIG3_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Ubiquinone biosynthesis hydroxylase, UbiH/UbiF/VisC/COQ6 family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">Rmet_3191</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Cofactor biosynthesis; ubiquinone biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the UbiH/COQ6 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10063.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3191"/>
    <dbReference type="EnsemblBacteria" id="ABF10063">
        <property type="protein sequence ID" value="ABF10063"/>
        <property type="gene ID" value="Rmet_3191"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3191"/>
    <dbReference type="eggNOG" id="COG0654">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009665_8_3_4"/>
    <dbReference type="OMA" id="SDVACII"/>
    <dbReference type="UniPathway" id="UPA00232"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016709">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, NAD(P)H as one donor, and incorporation of one atom of oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006744">
        <property type="term" value="P:ubiquinone biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002938">
        <property type="entry name" value="FAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018168">
        <property type="entry name" value="Ubi_Hdrlase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010971">
        <property type="entry name" value="UbiH/COQ6"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01494">
        <property type="entry name" value="FAD_binding_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="SSF51905"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01988">
        <property type="entry name" value="Ubi-OHases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01304">
        <property type="entry name" value="UBIH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0274">FAD</keyword>
    <keyword evidence="4" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="7" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0830">Ubiquinone</keyword>
    <feature type="domain" description="FAD_binding_3" evidence="9">
        <location>
            <begin position="63"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="39"/>
            <end position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004749"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ABF10063.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="474" mass="50955" checksum="CAF01D78BD9F78AF" modified="2006-05-30" version="1">MPASGGRVRRPGGKRQNRSVCVSEPITRMEPPSRRYNSGMSGPAASVSRTVTSSMSSNSSSRFQVAVVGGGIVGKACALLLAQQGMQVALAAPRPAAEGARRGGDDWDARIYAFSASSQALLERLRVWEALDPARVQPVRDMRVFGDESAAETDPSLDGDLHFSAYAAAVPQLAWIVESGHVERVLDTALRFQHQVKWFDDTATGLERDVDGVTLTLASGAQVRANLLVGADGSRSWVRQQCHIGTTQRRYRQLGVVANFQCELPHQETAWQWFLGAPEKLMADEEPANGEILAMLPLPGNRVSMVWSADEAHAHDLLALSPEALAATVAQVASGAVGARFGALRCITPAQGFPLVLQRAEQFVQPHVALVGDAAHVVHPLAGQGMNLGLRDVVELGRVMADKESFRSEGDLRLLRRYERARATDLLSLTAATDGLHRLFSLPGTPARVVRNLGMRAVGSQGLIKRLLIGHALG</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LIK4</accession>
    <name>Q1LIK4_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative zinc protease protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_3150</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10022.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517632.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3150"/>
    <dbReference type="EnsemblBacteria" id="ABF10022">
        <property type="protein sequence ID" value="ABF10022"/>
        <property type="gene ID" value="Rmet_3150"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3150"/>
    <dbReference type="eggNOG" id="COG4324">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_064960_0_0_4"/>
    <dbReference type="OMA" id="LYDQWVP"/>
    <dbReference type="OrthoDB" id="1357742at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008233">
        <property type="term" value="F:peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014553">
        <property type="entry name" value="Aminopept"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10023">
        <property type="entry name" value="Aminopep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF029285">
        <property type="entry name" value="Aminopept"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="364"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="371"/>
            <end position="385"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF10022.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="385" mass="43258" checksum="DBB9BA928442DCAF" modified="2006-05-30" version="1">MIRWPARRRLASALAVLGAALLVAGCDTLGYYYQSIGGHLGVMAQAQPIDDAIATANGAHDARLAQRLTLAREIRDYASRELKLPDNGSYRRYANLHRPYVVWSVFATPELSMDLRKWCFPIVGCITYRGYYDQKAADHYALGLRRDGLEAYVAGIPAYSTLGYFDDPLLNTFVYLPEGELARLIFHELAHQVVYVRNDTAFNESFATAVETAGVERWLAQEGSPEVRASYQIYDKRRRQFRTMLLGARDQLVELYHSPLDDDAKRQGKARIFDELRQTYANLKKDEWGGYSGYDRWFEQPLTNAHLAAVATYQQWVPAFTALLAQCDGDWDRFYAAARKIGDLPPAERDAALRKLAPPATEVKPLTALRATDAGSQTSATSQGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-10-07" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LJ16</accession>
    <name>Q1LJ16_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_2987</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09860.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2987"/>
    <dbReference type="EnsemblBacteria" id="ABF09860">
        <property type="protein sequence ID" value="ABF09860"/>
        <property type="gene ID" value="Rmet_2987"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2987"/>
    <dbReference type="HOGENOM" id="CLU_2082905_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="43"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF09860.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="117" mass="12755" checksum="046DA51DB8DA220B" modified="2006-05-30" version="1">MGRTLREHPTARLSLRVAQAPHGRDPIGTGHTRPTSPDAGASATSKWIARIIGFDGASYRLADWGGKHDKRPSRTAFCRKMVIDRNFDDATLLAETSLRLPLQSAYVAPSSQISKDA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-01-15" modified="2022-08-03" version="101" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LJ30</accession>
    <name>SYL_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Leucine--tRNA ligase</fullName>
            <ecNumber evidence="1">6.1.1.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Leucyl-tRNA synthetase</fullName>
            <shortName evidence="1">LeuRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">leuS</name>
        <name type="ordered locus">Rmet_2973</name>
    </gene>
    <organism>
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu)</text>
            <dbReference type="Rhea" id="RHEA:11688"/>
            <dbReference type="Rhea" id="RHEA-COMP:9613"/>
            <dbReference type="Rhea" id="RHEA-COMP:9622"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57427"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78494"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.4"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-I aminoacyl-tRNA synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.4" evidence="1"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09846.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517508.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q1LJ30"/>
    <dbReference type="SMR" id="Q1LJ30"/>
    <dbReference type="STRING" id="266264.Rmet_2973"/>
    <dbReference type="EnsemblBacteria" id="ABF09846">
        <property type="protein sequence ID" value="ABF09846"/>
        <property type="gene ID" value="Rmet_2973"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2973"/>
    <dbReference type="eggNOG" id="COG0495">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004427_0_0_4"/>
    <dbReference type="OMA" id="TFMVLAP"/>
    <dbReference type="OrthoDB" id="32262at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002161">
        <property type="term" value="F:aminoacyl-tRNA editing activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004823">
        <property type="term" value="F:leucine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006429">
        <property type="term" value="P:leucyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.740.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00049_B">
        <property type="entry name" value="Leu_tRNA_synth_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001412">
        <property type="entry name" value="aa-tRNA-synth_I_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002300">
        <property type="entry name" value="aa-tRNA-synth_Ia"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002302">
        <property type="entry name" value="Leu-tRNA-ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025709">
        <property type="entry name" value="Leu_tRNA-synth_edit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013155">
        <property type="entry name" value="M/V/L/I-tRNA-synth_anticd-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015413">
        <property type="entry name" value="Methionyl/Leucyl_tRNA_Synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009080">
        <property type="entry name" value="tRNAsynth_Ia_anticodon-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009008">
        <property type="entry name" value="Val/Leu/Ile-tRNA-synth_edit"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43740">
        <property type="entry name" value="PTHR43740"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08264">
        <property type="entry name" value="Anticodon_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00133">
        <property type="entry name" value="tRNA-synt_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13603">
        <property type="entry name" value="tRNA-synt_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09334">
        <property type="entry name" value="tRNA-synt_1g"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00985">
        <property type="entry name" value="TRNASYNTHLEU"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47323">
        <property type="entry name" value="SSF47323"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50677">
        <property type="entry name" value="SSF50677"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00396">
        <property type="entry name" value="leuS_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00178">
        <property type="entry name" value="AA_TRNA_LIGASE_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_1000009406" description="Leucine--tRNA ligase">
        <location>
            <begin position="1"/>
            <end position="873"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'HIGH' region">
        <location>
            <begin position="48"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'KMSKS' region">
        <location>
            <begin position="636"/>
            <end position="640"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="639"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00049"/>
        </source>
    </evidence>
    <sequence length="873" mass="96869" checksum="5641A1642E217597" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LJD6</accession>
    <name>Q1LJD6_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">ABC-type transporter, periplasmic component: HAAT family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">livK2</name>
        <name evidence="2" type="ordered locus">Rmet_2867</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09740.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517421.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2867"/>
    <dbReference type="EnsemblBacteria" id="ABF09740">
        <property type="protein sequence ID" value="ABF09740"/>
        <property type="gene ID" value="Rmet_2867"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2867"/>
    <dbReference type="eggNOG" id="COG0683">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027128_6_0_4"/>
    <dbReference type="OMA" id="YGVGFEQ"/>
    <dbReference type="OrthoDB" id="776281at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028081">
        <property type="entry name" value="Leu-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028082">
        <property type="entry name" value="Peripla_BP_I"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13458">
        <property type="entry name" value="Peripla_BP_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53822">
        <property type="entry name" value="SSF53822"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peripla_BP_6" evidence="1">
        <location>
            <begin position="51"/>
            <end position="389"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF09740.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="401" mass="41836" checksum="68AEAC2EDE013B4F" modified="2006-05-30" version="1">MQFTFAKILPIAAAVALVAACGKKEEKPVESAAPAPAATAAADTSGGETIVKIGHAAPLTGGIAHLGKDNENGARLAVEEVNKDGLTINGKKIKLELVGEDDAADPKTGTAVAQKLVDAKVVAVVGHLNSGVSIPASKIYSDAGIVQISPSSTNPDYTKQGFKTTYRVVATDAQQGPALANYAAKTLHAKTVAIVDDATAYGKGLADEFEKTSKADGVNVVAREATNDKATDFKAILTKIKGKKPDVIMYGGMDATGGPFAKQAKELGITAKIVGGDGVCTDKVAELAGDAVDNLICSEAGLALSKMEKGADFEKRYQARFNSPVQIYAPFTYDAVMVIVDAMKRANSTDAAAILAEMPKTNYKGVIGNIAFDEKGDMKEGTITLYDYKDKKKSVLDVVKM</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LJM2</accession>
    <name>Q1LJM2_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Threonine-phosphate decarboxylase</fullName>
            <ecNumber evidence="5">4.1.1.81</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">L-threonine-O-3-phosphate decarboxylase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">cobC</name>
        <name evidence="11" type="ordered locus">Rmet_2781</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Decarboxylates L-threonine-O-3-phosphate to yield (R)-1-amino-2-propanol O-2-phosphate, the precursor for the linkage between the nucleotide loop and the corrin ring in cobalamin.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>H(+) + O-phospho-L-threonine = (R)-1-aminopropan-2-yl phosphate + CO2</text>
            <dbReference type="Rhea" id="RHEA:11492"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:58563"/>
            <dbReference type="ChEBI" id="CHEBI:58675"/>
            <dbReference type="EC" id="4.1.1.81"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="4">Cofactor biosynthesis; adenosylcobalamin biosynthesis.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.81" evidence="5"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09654.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517352.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2781"/>
    <dbReference type="EnsemblBacteria" id="ABF09654">
        <property type="protein sequence ID" value="ABF09654"/>
        <property type="gene ID" value="Rmet_2781"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2781"/>
    <dbReference type="eggNOG" id="COG0079">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017584_3_4_4"/>
    <dbReference type="OMA" id="RDPWSVN"/>
    <dbReference type="OrthoDB" id="1248286at2"/>
    <dbReference type="UniPathway" id="UPA00148"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016874">
        <property type="term" value="F:ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048472">
        <property type="term" value="F:threonine-phosphate decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008483">
        <property type="term" value="F:transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009236">
        <property type="term" value="P:cobalamin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005860">
        <property type="entry name" value="CobD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="SSF53383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01140">
        <property type="entry name" value="L_thr_O3P_dcar"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="11" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="6" id="KW-0169">Cobalamin biosynthesis</keyword>
    <keyword evidence="11" id="KW-0436">Ligase</keyword>
    <keyword evidence="8" id="KW-0456">Lyase</keyword>
    <keyword evidence="7" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aminotran_1_2" evidence="10">
        <location>
            <begin position="72"/>
            <end position="317"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001790"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00003444"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022573"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00029996"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ABF09654.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="343" mass="36105" checksum="8C650A098B2C97C8" modified="2006-05-30" version="1">MTHAPIRHGGNLLAAVRRYGRPAETWLDLSTGINPDGYPVPLLPADAWQRLPQDDDGLAGIAAQAYGAAHALPVAGSQAAIRTLPRLLRPGRVGIAALGYSEYAPAFAQAGHTVTPLDEADFQRADLADALDHLVVVNPNNPTARLLPIDTLRHWHERLASRGGTLIVDEAFIDCTGAASLAGSSAAPGLIVLRSLGKFYGLAGVRCGFVLAQPALLDALADQLGHWTVSGPARAVARIALTDTAWQAATRTSLSAAGQRLAILLRDHGLSPVSQPLFSWVPDARAGSLHEALARQGVWTRLFDAAGGCPSSLRFGLPPNDDAAWQRLDAALGHARHALPSLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LJM6</accession>
    <name>Q1LJM6_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Cobyrinic acid a,c-diamide synthase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">cbiA</name>
        <name evidence="2" type="ordered locus">Rmet_2777</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09650.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517348.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2777"/>
    <dbReference type="EnsemblBacteria" id="ABF09650">
        <property type="protein sequence ID" value="ABF09650"/>
        <property type="gene ID" value="Rmet_2777"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2777"/>
    <dbReference type="eggNOG" id="COG1192">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037612_5_0_4"/>
    <dbReference type="OMA" id="ADHIAKP"/>
    <dbReference type="OrthoDB" id="1631847at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002586">
        <property type="entry name" value="CobQ/CobB/MinD/ParA_Nub-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01656">
        <property type="entry name" value="CbiA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CbiA" evidence="1">
        <location>
            <begin position="4"/>
            <end position="182"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF09650.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="220" mass="24044" checksum="2ED6F88842F56232" modified="2006-05-30" version="1">MPVVVIANPKGGVGKTTLATNLAGYFARHGHAVMLGDTDRQQSSRAWLNLRPESAPPISTWDIDADNIARPPKGTTHVVLDTPAGLHGWRYSDVMKLAHRVVVPLQPSMFDILATQDFLAKLAGDKRVRHGEVPVGVIGMRVDVRTRAAEQLQRFVEGLGLPVLGYLRDTQNYVQLAAHGLTLWDVAPSRVARDWEQWQPILRWLDGKGGVVTEGPAAVG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-12-12" modified="2022-05-25" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LJX2</accession>
    <name>NRDR_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Transcriptional repressor NrdR</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">nrdR</name>
        <name type="ordered locus">Rmet_2681</name>
    </gene>
    <organism>
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Negatively regulates transcription of bacterial ribonucleotide reductase nrd genes and operons by binding to NrdR-boxes.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="1">Binds 1 zinc ion.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the NrdR family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09554.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517254.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q1LJX2"/>
    <dbReference type="SMR" id="Q1LJX2"/>
    <dbReference type="STRING" id="266264.Rmet_2681"/>
    <dbReference type="EnsemblBacteria" id="ABF09554">
        <property type="protein sequence ID" value="ABF09554"/>
        <property type="gene ID" value="Rmet_2681"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2681"/>
    <dbReference type="eggNOG" id="COG1327">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_108412_0_0_4"/>
    <dbReference type="OMA" id="YRFTTYE"/>
    <dbReference type="OrthoDB" id="1551627at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045892">
        <property type="term" value="P:negative regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00440">
        <property type="entry name" value="NrdR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005144">
        <property type="entry name" value="ATP-cone_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003796">
        <property type="entry name" value="RNR_NrdR-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30455">
        <property type="entry name" value="PTHR30455"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03477">
        <property type="entry name" value="ATP-cone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00244">
        <property type="entry name" value="TIGR00244"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51161">
        <property type="entry name" value="ATP_CONE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0238">DNA-binding</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0678">Repressor</keyword>
    <keyword id="KW-0804">Transcription</keyword>
    <keyword id="KW-0805">Transcription regulation</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <keyword id="KW-0863">Zinc-finger</keyword>
    <feature type="chain" id="PRO_0000264198" description="Transcriptional repressor NrdR">
        <location>
            <begin position="1"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="domain" description="ATP-cone" evidence="1">
        <location>
            <begin position="49"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="zinc finger region" evidence="1">
        <location>
            <begin position="3"/>
            <end position="34"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00440"/>
        </source>
    </evidence>
    <sequence length="155" mass="17977" checksum="D215288265AB20E9" modified="2006-05-30" version="1">MKCPFCGHSSTQVLDSRVSEDGDTVRRRRRCEACDRRFTTYERIELFFPAIVKKNGSRVDYARAKLKDSMRLALRKRPVSAEAIDEAITRIEEKLLALGEKEIPSSQVGELVMRELRKLDKIAYIRFASVYRSFEDVSEFREMLDEFSSSTPRKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="103" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LK01</accession>
    <name>Q1LK01_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 5">Kynureninase</fullName>
            <ecNumber evidence="4 5">3.7.1.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">L-kynurenine hydrolase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="4 7" type="primary">kynU</name>
        <name evidence="7" type="ordered locus">Rmet_2650</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4 5">Catalyzes the cleavage of L-kynurenine (L-Kyn) and L-3-hydroxykynurenine (L-3OHKyn) into anthranilic acid (AA) and 3-hydroxyanthranilic acid (3-OHAA), respectively.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>3-hydroxy-L-kynurenine + H2O = 3-hydroxyanthranilate + H(+) + L-alanine</text>
            <dbReference type="Rhea" id="RHEA:25143"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:36559"/>
            <dbReference type="ChEBI" id="CHEBI:57972"/>
            <dbReference type="ChEBI" id="CHEBI:58125"/>
            <dbReference type="EC" id="3.7.1.3"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4 5">
            <text>H2O + L-kynurenine = anthranilate + H(+) + L-alanine</text>
            <dbReference type="Rhea" id="RHEA:16813"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16567"/>
            <dbReference type="ChEBI" id="CHEBI:57959"/>
            <dbReference type="ChEBI" id="CHEBI:57972"/>
            <dbReference type="EC" id="3.7.1.3"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="4 5">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="4 5">Amino-acid degradation; L-kynurenine degradation; L-alanine and anthranilate from L-kynurenine: step 1/1.</text>
    </comment>
    <comment type="pathway">
        <text evidence="4 5">Cofactor biosynthesis; NAD(+) biosynthesis; quinolinate from L-kynurenine: step 2/3.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4 5">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 5">Belongs to the kynureninase family.</text>
    </comment>
    <dbReference type="EC" id="3.7.1.3" evidence="4 5"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09525.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517224.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2650"/>
    <dbReference type="EnsemblBacteria" id="ABF09525">
        <property type="protein sequence ID" value="ABF09525"/>
        <property type="gene ID" value="Rmet_2650"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2650"/>
    <dbReference type="eggNOG" id="COG3844">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003433_4_1_4"/>
    <dbReference type="OMA" id="TFPTDGY"/>
    <dbReference type="OrthoDB" id="1474443at2"/>
    <dbReference type="UniPathway" id="UPA00253">
        <property type="reaction ID" value="UER00329"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00334">
        <property type="reaction ID" value="UER00455"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061981">
        <property type="term" value="F:3-hydroxykynureninase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030429">
        <property type="term" value="F:kynureninase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034354">
        <property type="term" value="P:'de novo' NAD biosynthetic process from tryptophan"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043420">
        <property type="term" value="P:anthranilate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097053">
        <property type="term" value="P:L-kynurenine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019805">
        <property type="term" value="P:quinolinate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006569">
        <property type="term" value="P:tryptophan catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01970">
        <property type="entry name" value="Kynureninase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000192">
        <property type="entry name" value="Aminotrans_V_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010111">
        <property type="entry name" value="Kynureninase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14084">
        <property type="entry name" value="PTHR14084"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00266">
        <property type="entry name" value="Aminotran_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038800">
        <property type="entry name" value="KYNU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="SSF53383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01814">
        <property type="entry name" value="kynureninase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4 5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1 4 5" id="KW-0662">Pyridine nucleotide biosynthesis</keyword>
    <keyword evidence="3 4 5" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminotran_5" evidence="6">
        <location>
            <begin position="78"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="region of interest" description="Pyridoxal phosphate binding" evidence="4">
        <location>
            <begin position="131"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="99"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="100"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="174"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="203"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="206"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="228"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="258"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="284"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="4">
        <location>
            <position position="229"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRNR" id="PIRNR038800"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABF09525.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="418" mass="45471" checksum="9B4B33EF694EEC37" modified="2006-05-30" version="1">MTTLTRAHCQTLDRDDPLRALREQFALPEGVIYLDGNSLGARPRAAAARAAEVVSEEWGNGLIRSWNTAGWFDLPRRLGNLLAPLIGAGNDEVVVTDTTSINLFKVLAAALRVQRQRDPARRVIVSEASNFPTDLYIAQGLADLLQQGYEVRLVNSPDEIDAAVGADTAVLMLTHVNYKTGEMLDMAGLTELAHARGVLTVWDLAHSAGAVPVALNVAKADYAIGCTYKYLNGGPGSPAFVWVAPALRDQFWQPLSGWWGHGAPFAMSPQYEPAQGIGRFLCGTQPMTSLAMVECGLDIFAQTSMTALRAKSLKLTDLFIELVEARCATHPLSLVTPRDHTKRGSQVSFAHPDAYALVAALIERGVIGDYREPGIARFGFTPLYTSFTEVWDAVEIIRDVLDSGVYRDARFAVRTAVT</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LK52</accession>
    <name>Q1LK52_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Alpha-methylacyl-CoA racemase MCR (2-methylacyl-CoA racemase) (2-arylpropionyl-CoA epimerase )</fullName>
            <ecNumber evidence="1">5.1.99.4</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">mcr</name>
        <name evidence="1" type="ordered locus">Rmet_2597</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EC" id="5.1.99.4" evidence="1"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09474.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517177.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2597"/>
    <dbReference type="EnsemblBacteria" id="ABF09474">
        <property type="protein sequence ID" value="ABF09474"/>
        <property type="gene ID" value="Rmet_2597"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2597"/>
    <dbReference type="eggNOG" id="COG1804">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033975_5_1_4"/>
    <dbReference type="OMA" id="HWMNPRL"/>
    <dbReference type="OrthoDB" id="969868at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008111">
        <property type="term" value="F:alpha-methylacyl-CoA racemase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1540.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10540">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003673">
        <property type="entry name" value="CoA-Trfase_fam_III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044855">
        <property type="entry name" value="CoA-Trfase_III_dom3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023606">
        <property type="entry name" value="CoA-Trfase_III_dom_1_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02515">
        <property type="entry name" value="CoA_transf_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89796">
        <property type="entry name" value="SSF89796"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0413">Isomerase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF09474.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="367" mass="38717" checksum="A346B616F755FC10" modified="2006-05-30" version="1">MSTSGATLAAPAAPAANAAAAPPLAGVRVVDLTELLPGPFLTQTLLEMGAEVVKIERPPHGDNARRLAPGLFAALNRGKHSLYCDLKSDSGLAEVRAQIAQADILIESYRPGAMRRLGLDYESLREQFPRLIYLSLTGFGQDGPHALLPGHDINYLAAAGALSLTGTPGGPPVHQAMLPIADLAGASMALSALLAALYQRTGSGRGQYLDAALADSVLHWMAPRLGLFRQKAATTADAQRAMLGGKPAYGVFRCRDGRYLTIAALEEHFWRGLNDVLGLFAANDACCVDHAARTANAETINARLAERVAEYDADTLMARLANADLPVFPVLDAVEAVAQARHRSGARITETEDGPLPGFPVTLEGMA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LK70</accession>
    <name>Q1LK70_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Rmet_2579</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the pirin family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09456.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517159.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2579"/>
    <dbReference type="EnsemblBacteria" id="ABF09456">
        <property type="protein sequence ID" value="ABF09456"/>
        <property type="gene ID" value="Rmet_2579"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2579"/>
    <dbReference type="eggNOG" id="COG1741">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_064194_2_2_4"/>
    <dbReference type="OMA" id="ERGYADH"/>
    <dbReference type="OrthoDB" id="729805at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012093">
        <property type="entry name" value="Pirin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003829">
        <property type="entry name" value="Pirin_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041602">
        <property type="entry name" value="Quercetinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011051">
        <property type="entry name" value="RmlC_Cupin_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43212">
        <property type="entry name" value="PTHR43212"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02678">
        <property type="entry name" value="Pirin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17954">
        <property type="entry name" value="Pirin_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006232">
        <property type="entry name" value="Pirin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51182">
        <property type="entry name" value="SSF51182"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pirin" evidence="3">
        <location>
            <begin position="7"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="domain" description="Pirin_C_2" evidence="4">
        <location>
            <begin position="146"/>
            <end position="231"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008416"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU003457"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF02678"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF17954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABF09456.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="232" mass="25666" checksum="DC30C27FB5D5DE14" modified="2006-05-30" version="1">MIEIRKSEERGYADHGWLKSYHSFSFADYYDPRHVQFRSLRVINEDRVAPGMGFGTHGHRDMEIISYVLEGELAHKDSMGNGSVIRPGDVQRMSAGTGVRHSEYNHAAHDTTHFLQIWIMPNETGIEPGYEEKRFEAADKRGRLRLVGSPDGAEGSVVIHQDVRLYAGLFDGDEAATLSLAPGRRAYVHVARGRISVNGQVLEAGDAAKLEDVETVNLSQGSGAEVLVFDLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="109" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LKI2</accession>
    <name>Q1LKI2_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Tryptophan synthase beta chain</fullName>
            <ecNumber evidence="11">4.2.1.20</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11 13" type="primary">trpB</name>
        <name evidence="13" type="ordered locus">Rmet_2467</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">The beta subunit is responsible for the synthesis of L-tryptophan from indole and L-serine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 11">
            <text>(1S,2R)-1-C-(indol-3-yl)glycerol 3-phosphate + L-serine = D-glyceraldehyde 3-phosphate + H2O + L-tryptophan</text>
            <dbReference type="Rhea" id="RHEA:10532"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
            <dbReference type="ChEBI" id="CHEBI:57912"/>
            <dbReference type="ChEBI" id="CHEBI:58866"/>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
            <dbReference type="EC" id="4.2.1.20"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 11">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3 11">Amino-acid biosynthesis; L-tryptophan biosynthesis; L-tryptophan from chorismate: step 5/5.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5 11">Tetramer of two alpha and two beta chains.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 11">Belongs to the TrpB family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.20" evidence="11"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09344.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2467"/>
    <dbReference type="EnsemblBacteria" id="ABF09344">
        <property type="protein sequence ID" value="ABF09344"/>
        <property type="gene ID" value="Rmet_2467"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2467"/>
    <dbReference type="eggNOG" id="COG0133">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016734_3_1_4"/>
    <dbReference type="OMA" id="HGMKSYF"/>
    <dbReference type="UniPathway" id="UPA00035">
        <property type="reaction ID" value="UER00044"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004834">
        <property type="term" value="F:tryptophan synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd06446">
        <property type="entry name" value="Trp-synth_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1100">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00133">
        <property type="entry name" value="Trp_synth_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001926">
        <property type="entry name" value="PLP-dep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006653">
        <property type="entry name" value="Trp_synth_b_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006654">
        <property type="entry name" value="Trp_synth_beta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023026">
        <property type="entry name" value="Trp_synth_beta/beta-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036052">
        <property type="entry name" value="Trypto_synt_PLP_dependent"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00291">
        <property type="entry name" value="PALP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001413">
        <property type="entry name" value="Trp_syn_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53686">
        <property type="entry name" value="SSF53686"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00263">
        <property type="entry name" value="trpB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00168">
        <property type="entry name" value="TRP_SYNTHASE_BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 11" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="9 11" id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword evidence="10 11 13" id="KW-0456">Lyase</keyword>
    <keyword evidence="8 11" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 11" id="KW-0822">Tryptophan biosynthesis</keyword>
    <feature type="domain" description="PALP" evidence="12">
        <location>
            <begin position="66"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="11">
        <location>
            <position position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ABF09344.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="407" mass="44297" checksum="6643C796F7F1D06E" modified="2006-05-30" version="1">MADALPESLDMYDLPDSRGHFGPYGGTFVSETLVHALDELRDAYARYQKDPDFDAEYRRELKHFVGRPSPIYHAQRWSETLGGAQIYLKREDLNHTGAHKINNVIGQALLAKRMGKKRVIAETGAGQHGVATATIAARFGMECVVYMGSEDVRRQAANVYRMKLLGATVVPVESGSRTLKDALNEAMRDWVTNVETTFYIIGTVAGPHPYPMMVRDFQCVIGEEAKVQMPELAGRQPDAVIACVGGGSNAMGIFYPYIEHKDVQLIGVEAAGDGLDSGRHAAALIGGSPGVLHGNRTYLLQDANGQIIETHSVSAGLDYPGVGPEHAWLKDMGRAQYVPITDEEALKAFHDCCRIEGIIPALESSHAIAYACKLAPTLGRDKLLLVNLSGRGDKDMHTVAERAGLKL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-05-20" modified="2022-08-03" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LKN1</accession>
    <name>RNC_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Ribonuclease 3</fullName>
            <ecNumber evidence="1">3.1.26.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Ribonuclease III</fullName>
            <shortName evidence="1">RNase III</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rnc</name>
        <name type="ordered locus">Rmet_2418</name>
    </gene>
    <organism>
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Digests double-stranded RNA. Involved in the processing of primary rRNA transcript to yield the immediate precursors to the large and small rRNAs (23S and 16S). Processes some mRNAs, and tRNAs when they are encoded in the rRNA operon. Processes pre-crRNA and tracrRNA of type II CRISPR loci if present in the organism.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Endonucleolytic cleavage to 5'-phosphomonoester.</text>
            <dbReference type="EC" id="3.1.26.3"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the ribonuclease III family.</text>
    </comment>
    <dbReference type="EC" id="3.1.26.3" evidence="1"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09295.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008644793.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q1LKN1"/>
    <dbReference type="SMR" id="Q1LKN1"/>
    <dbReference type="STRING" id="266264.Rmet_2418"/>
    <dbReference type="EnsemblBacteria" id="ABF09295">
        <property type="protein sequence ID" value="ABF09295"/>
        <property type="gene ID" value="Rmet_2418"/>
    </dbReference>
    <dbReference type="GeneID" id="60821108"/>
    <dbReference type="KEGG" id="rme:Rmet_2418"/>
    <dbReference type="eggNOG" id="COG0571">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000907_1_1_4"/>
    <dbReference type="OMA" id="LTHKSCK"/>
    <dbReference type="OrthoDB" id="1890943at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004525">
        <property type="term" value="F:ribonuclease III activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008033">
        <property type="term" value="P:tRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00593">
        <property type="entry name" value="RIBOc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1520.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00104">
        <property type="entry name" value="RNase_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014720">
        <property type="entry name" value="dsRBD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011907">
        <property type="entry name" value="RNase_III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000999">
        <property type="entry name" value="RNase_III_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036389">
        <property type="entry name" value="RNase_III_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11207">
        <property type="entry name" value="PTHR11207"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00035">
        <property type="entry name" value="dsrm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14622">
        <property type="entry name" value="Ribonucleas_3_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00358">
        <property type="entry name" value="DSRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00535">
        <property type="entry name" value="RIBOc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69065">
        <property type="entry name" value="SSF69065"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02191">
        <property type="entry name" value="RNaseIII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50137">
        <property type="entry name" value="DS_RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00517">
        <property type="entry name" value="RNASE_3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50142">
        <property type="entry name" value="RNASE_3_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0255">Endonuclease</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0507">mRNA processing</keyword>
    <keyword id="KW-0540">Nuclease</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0698">rRNA processing</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <keyword id="KW-0819">tRNA processing</keyword>
    <feature type="chain" id="PRO_1000075794" description="Ribonuclease 3">
        <location>
            <begin position="1"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="domain" description="RNase III" evidence="1">
        <location>
            <begin position="3"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="domain" description="DRBM" evidence="1">
        <location>
            <begin position="152"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="229"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="42"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="114"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="38"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="111"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="114"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="256" mass="28412" checksum="D226B8538CB95EC8" modified="2006-05-30" version="1">MTLDALQQRLGYRFSKPELLQQALTHRSHSAQHNERLEFLGDSVLNCAVADMLFGMFGKLDEGDLSRVRANLVKQQALYEIAQMLQLSDALRLGEGELKSGGFRRPSILADALEAIVGAVFLDAGFDAARALIRKLYIPILEQVDPRTLGKDAKTLLQEYLQGHKIALPQYNVIATHGAAHSQQFEVECIVPKLEVRVFGTGASRRAAEQAAAKLALDEVQKLVPQLLKRSRAERTGKTRKQPVPPDPQLSLRLKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="66" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LKP8</accession>
    <name>Q1LKP8_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative HTH-type transcriptional regulator yazB (Modular protein)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_2401</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09278.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_003149846.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2401"/>
    <dbReference type="EnsemblBacteria" id="ABF09278">
        <property type="protein sequence ID" value="ABF09278"/>
        <property type="gene ID" value="Rmet_2401"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2401"/>
    <dbReference type="eggNOG" id="COG1813">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_066192_29_0_4"/>
    <dbReference type="OMA" id="SRSHMGK"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00093">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001387">
        <property type="entry name" value="Cro/C1-type_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010982">
        <property type="entry name" value="Lambda_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01381">
        <property type="entry name" value="HTH_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00530">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47413">
        <property type="entry name" value="SSF47413"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50943">
        <property type="entry name" value="HTH_CROC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH cro/C1-type" evidence="1">
        <location>
            <begin position="29"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF09278.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="97" mass="10192" checksum="2232EFD32E477359" modified="2006-05-30" version="1">MQKSTIQPGRPSGTTTYESAAATAFGQAVRAARVAQGVAQDEFASRAGISRSHMGKIERGEHVPTLPLILKISVALGISAAELMAATERNLRAETDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LKQ1</accession>
    <name>Q1LKQ1_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Putative pyridine nucleotide-disulfide oxidoreductase, class I</fullName>
            <ecNumber evidence="4">1.8.1.4</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Rmet_2398</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <dbReference type="EC" id="1.8.1.4" evidence="4"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09275.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004350672.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2398"/>
    <dbReference type="EnsemblBacteria" id="ABF09275">
        <property type="protein sequence ID" value="ABF09275"/>
        <property type="gene ID" value="Rmet_2398"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2398"/>
    <dbReference type="eggNOG" id="COG1249">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016755_0_3_4"/>
    <dbReference type="OrthoDB" id="267896at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004148">
        <property type="term" value="F:dihydrolipoyl dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045454">
        <property type="term" value="P:cell redox homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.390.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023753">
        <property type="entry name" value="FAD/NAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016156">
        <property type="entry name" value="FAD/NAD-linked_Rdtase_dimer_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004099">
        <property type="entry name" value="Pyr_nucl-diS_OxRdtase_dimer"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07992">
        <property type="entry name" value="Pyr_redox_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02852">
        <property type="entry name" value="Pyr_redox_dim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="SSF51905"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55424">
        <property type="entry name" value="SSF55424"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pyr_redox_2" evidence="3">
        <location>
            <begin position="7"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="domain" description="Pyr_redox_dim" evidence="2">
        <location>
            <begin position="344"/>
            <end position="448"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF07992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF09275.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="458" mass="47692" checksum="967CA45CC9043561" modified="2006-05-30" version="1">MQTRKVDVAIIGAGTAGSTAFHALKATGKQVVLIDRGPLGTTCARVGCMPSKAALHAGMHWKVAKALAASATPSTQGPQALWSHARQTRDALAGAAAERTRKAAGESLIMAEARFVAPGILQAGEQRIEASAFIVATGSRPVVPKALDGVASRILTTDTLFDLGVLPARMGILGLGAIGLELGLALSRLDVQVVAADQKDAIGGIQDPDVLERAIARFETELPMWLGAAVEVSLEGDQVRMRAGERDALVDRLLVVTGRQPNTEALDLAAAGISLDQAGRPSIDPATMQACGPSQVPIFFAGDVQPDRPLMHEAADEGQMAAQAALASLRGESWPGASRRVPITILFTDPDACAVGMTYEAAVQEGAVVGTAEGSGNGRSKILGAPENLLHIYADPGSGALLGASMLLTQGEHLAHLIAWAIQAKQTVNDLLAMPYYHPSIEEMLQSALKSASQQMRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="59" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LKV3</accession>
    <name>Q1LKV3_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_2346</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09223.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_003050225.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2346"/>
    <dbReference type="EnsemblBacteria" id="ABF09223">
        <property type="protein sequence ID" value="ABF09223"/>
        <property type="gene ID" value="Rmet_2346"/>
    </dbReference>
    <dbReference type="GeneID" id="56409854"/>
    <dbReference type="GeneID" id="61799662"/>
    <dbReference type="KEGG" id="rme:Rmet_2346"/>
    <dbReference type="eggNOG" id="ENOG5032RJK">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_195279_1_0_4"/>
    <dbReference type="OrthoDB" id="2015550at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021676">
        <property type="entry name" value="DUF3262"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11660">
        <property type="entry name" value="DUF3262"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03758">
        <property type="entry name" value="conj_TIGR03758"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="57"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF09223.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="77" mass="8401" checksum="54C62FF338A48FFB" modified="2006-05-30" version="1">MNGAQVSAFQANSGIAPSAMATVLVGVVFAVLLVWGVWAIRTAYVGWSESRLNQRQFLGVCIRFVAMYLVLSFFLLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-04-07" version="58" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LL06</accession>
    <name>Q1LL06_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_2291</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09170.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_003050422.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2291"/>
    <dbReference type="EnsemblBacteria" id="ABF09170">
        <property type="protein sequence ID" value="ABF09170"/>
        <property type="gene ID" value="Rmet_2291"/>
    </dbReference>
    <dbReference type="GeneID" id="56409800"/>
    <dbReference type="KEGG" id="rme:Rmet_2291"/>
    <dbReference type="eggNOG" id="ENOG5032U3U">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_196073_0_0_4"/>
    <dbReference type="OMA" id="GMPPIFT"/>
    <dbReference type="OrthoDB" id="1938295at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="69"/>
            <end position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF09170.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="89" mass="9563" checksum="CEFF53D1D8458540" modified="2006-05-30" version="1">MANAHELARGHKRLRALIEFAVGEGWHVKRTPGGHLKFTKAGCAAIYTSSTASDHRAELNARAQIRRAEREARMAPAGGRGDCEGCGHG</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="106" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LLB7</accession>
    <name>Q1LLB7_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Phosphate regulon transcriptional regulatory protein PhoB</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">phoB</name>
        <name evidence="10" type="ordered locus">Rmet_2180</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">This protein is a positive regulator for the phosphate regulon. Transcription of this operon is positively regulated by PhoB and PhoR when phosphate is limited.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09059.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008641662.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2180"/>
    <dbReference type="EnsemblBacteria" id="ABF09059">
        <property type="protein sequence ID" value="ABF09059"/>
        <property type="gene ID" value="Rmet_2180"/>
    </dbReference>
    <dbReference type="GeneID" id="60821247"/>
    <dbReference type="KEGG" id="rme:Rmet_2180"/>
    <dbReference type="eggNOG" id="COG0745">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_30_4_4"/>
    <dbReference type="OMA" id="IDRVWGS"/>
    <dbReference type="OrthoDB" id="817710at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000156">
        <property type="term" value="F:phosphorelay response regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006817">
        <property type="term" value="P:phosphate ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00383">
        <property type="entry name" value="trans_reg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001867">
        <property type="entry name" value="OmpR/PhoB-type_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016032">
        <property type="entry name" value="Sig_transdc_resp-reg_C-effctor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011879">
        <property type="entry name" value="Sig_transdc_resp-reg_PhoB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039420">
        <property type="entry name" value="WalR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48111">
        <property type="entry name" value="PTHR48111"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00486">
        <property type="entry name" value="Trans_reg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00862">
        <property type="entry name" value="Trans_reg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46894">
        <property type="entry name" value="SSF46894"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02154">
        <property type="entry name" value="PhoB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51755">
        <property type="entry name" value="OMPR_PHOB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 7 10" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0592">Phosphate transport</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Response regulatory" evidence="8">
        <location>
            <begin position="4"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="domain" description="OmpR/PhoB-type" evidence="9">
        <location>
            <begin position="129"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="OmpR/PhoB-type" evidence="7">
        <location>
            <begin position="129"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="6">
        <location>
            <position position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00013332"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00024735"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ABF09059.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="235" mass="26193" checksum="35A4F0C86CCBF5C2" modified="2006-05-30" version="1">MPSSILVVEDEPAIAELIAVNLQHAGHYPIRAYNAEQALSLMSDVLPDLVLLDWMLPGKSGATFAKELRNNDRTKQIPIIMLTARSEEQDKVMGLEAGADDYVTKPFSPKELLARIKAVLRRRAPQLTDDVVAINGLRLDPATHRVTGQDDGGPIKLDLGPTEFRLLHFLMTHPERVHSRSQLLDQVWGDHVFVEERTVDVHIKRLRAALTPGGYSNMIETVRGSGYRLARSPSH</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-10-31" modified="2022-05-25" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LLX1</accession>
    <name>RL9_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">50S ribosomal protein L9</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rplI</name>
        <name type="ordered locus">Rmet_1976</name>
    </gene>
    <organism>
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Binds to the 23S rRNA.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the bacterial ribosomal protein bL9 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08855.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516698.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q1LLX1"/>
    <dbReference type="SMR" id="Q1LLX1"/>
    <dbReference type="STRING" id="266264.Rmet_1976"/>
    <dbReference type="EnsemblBacteria" id="ABF08855">
        <property type="protein sequence ID" value="ABF08855"/>
        <property type="gene ID" value="Rmet_1976"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1976"/>
    <dbReference type="eggNOG" id="COG0359">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_078938_4_1_4"/>
    <dbReference type="OMA" id="MKIILTH"/>
    <dbReference type="OrthoDB" id="1959318at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.430.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.5.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00503">
        <property type="entry name" value="Ribosomal_L9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000244">
        <property type="entry name" value="Ribosomal_L9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009027">
        <property type="entry name" value="Ribosomal_L9/RNase_H1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020594">
        <property type="entry name" value="Ribosomal_L9_bac/chp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020069">
        <property type="entry name" value="Ribosomal_L9_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036791">
        <property type="entry name" value="Ribosomal_L9_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020070">
        <property type="entry name" value="Ribosomal_L9_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036935">
        <property type="entry name" value="Ribosomal_L9_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21368">
        <property type="entry name" value="PTHR21368"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03948">
        <property type="entry name" value="Ribosomal_L9_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01281">
        <property type="entry name" value="Ribosomal_L9_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55653">
        <property type="entry name" value="SSF55653"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55658">
        <property type="entry name" value="SSF55658"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00158">
        <property type="entry name" value="L9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00651">
        <property type="entry name" value="RIBOSOMAL_L9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <feature type="chain" id="PRO_0000258478" description="50S ribosomal protein L9">
        <location>
            <begin position="1"/>
            <end position="150"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00503"/>
        </source>
    </evidence>
    <sequence length="150" mass="15964" checksum="2A1CE52135276418" modified="2006-05-30" version="1">MQVILLEKVINLGSLGDIVRVKDGYARNFLIPTKRARRATQSAIAEFEVKRAELEKVAAEKLAAAQAEGEKLNGLTVQITQKSGVDGRLFGSVTNADIAEALAGQGFKLEKAQVRMPSGPLKVVGDHPVSVALHTDVVVDVTVAVLGEHV</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LM76</accession>
    <name>Q1LM76_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative transcription regulator (MarR family)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_1871</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08750.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516596.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1871"/>
    <dbReference type="EnsemblBacteria" id="ABF08750">
        <property type="protein sequence ID" value="ABF08750"/>
        <property type="gene ID" value="Rmet_1871"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1871"/>
    <dbReference type="eggNOG" id="COG5631">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_111108_1_0_4"/>
    <dbReference type="OMA" id="HVNHRGR"/>
    <dbReference type="OrthoDB" id="1311154at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000835">
        <property type="entry name" value="HTH_MarR-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014601">
        <property type="entry name" value="Trans_reg_MarR_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13463">
        <property type="entry name" value="HTH_27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036158">
        <property type="entry name" value="UCP036158_MarR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH marR-type" evidence="2">
        <location>
            <begin position="77"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13463"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF08750.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="195" mass="21042" checksum="44E8C051BA73FBFA" modified="2006-05-30" version="1">MARKPVSDSPANAEPVASPSPAAPLARAAGVNIVSSSHLVSVRSPELSEFEFGLNTAYNAYSRWVVRCMGAAGVRDLTFLDVLVLHHVNHRGRAKRLADICFVLNVEDTHLVTYALKKLVGLGLVAGERVGKEATYATTQAGADACARYREIREQCLTSNFTEGSEENLEIGELARLLRVLTGLYEQAARSATSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LMC3</accession>
    <name>Q1LMC3_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Ankyrin repeat protein (ANK)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">arp3</name>
        <name evidence="3" type="ordered locus">Rmet_1824</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08703.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1824"/>
    <dbReference type="EnsemblBacteria" id="ABF08703">
        <property type="protein sequence ID" value="ABF08703"/>
        <property type="gene ID" value="Rmet_1824"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1824"/>
    <dbReference type="eggNOG" id="COG0666">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000134_18_0_4"/>
    <dbReference type="OMA" id="QKILLCM"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01415">
        <property type="entry name" value="ANKYRIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="SSF48403"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="repeat" description="ANK" evidence="1">
        <location>
            <begin position="111"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="1">
        <location>
            <begin position="144"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="205"/>
            <end position="226"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF08703.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="226" mass="24464" checksum="4B353E4F22CFE155" modified="2006-05-30" version="1">MALICAACASVAAPADDMRKAVEFDDANTVQKLLARGVDPNLVDNRGDPMLVVALREKSTKAATALIKAKNIDFDKTNPAGENPLMMASLQGELDMVKLMVDGMEAEVNKKGWAPLHYAATNGHNDVVKYLVDHAAYIDAESPNGTTPLMMAARGGHIDTVKLLLDEGADMRLKNQQNMTVIDFAEQYNQKEIADGLKSRWQKLYGAVPPPAPKPKPWTPPAGQAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LMM2</accession>
    <name>Q1LMM2_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Enoyl-CoA hydratase/isomerase</fullName>
            <ecNumber evidence="3">4.2.1.17</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_1723</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the enoyl-CoA hydratase/isomerase family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.17" evidence="3"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08604.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516457.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1723"/>
    <dbReference type="EnsemblBacteria" id="ABF08604">
        <property type="protein sequence ID" value="ABF08604"/>
        <property type="gene ID" value="Rmet_1723"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1723"/>
    <dbReference type="eggNOG" id="COG1024">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009834_7_3_4"/>
    <dbReference type="OMA" id="LTRIGRC"/>
    <dbReference type="OrthoDB" id="1114675at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004300">
        <property type="term" value="F:enoyl-CoA hydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016853">
        <property type="term" value="F:isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.12.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029045">
        <property type="entry name" value="ClpP/crotonase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001753">
        <property type="entry name" value="Enoyl-CoA_hydra/iso"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014748">
        <property type="entry name" value="Enoyl-CoA_hydra_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00378">
        <property type="entry name" value="ECH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52096">
        <property type="entry name" value="SSF52096"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0413">Isomerase</keyword>
    <keyword evidence="3" id="KW-0456">Lyase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF08604.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="277" mass="28852" checksum="B3D8397554AEE376" modified="2006-05-30" version="1">MTNPVIVRCVEGVLFATLNIPATRNALAPEVVAVLTDIVAQAEADVGIRALVLRGAGGTFSAGGNVGNFQARLDADVGHEDPVAARNRQFGHFMTRLATLPIPVIAAVDGPAMGGGMGMACAADVVLATRAARFALSETSLGIIPAQIAPFVVGRLGAALTRRLGLTGERLDGVAAEAVGLVDQLADDAAGLDELIAEWLTRIGRCGPHANRQFKALVARCGQTPVDSLLDDASHQFAQCMRTEGTEGIAAFREKREARWVTRFDAGIVCAAQSTDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-10-07" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LMM5</accession>
    <name>Q1LMM5_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Putative transcriptional regulator, IclR family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Rmet_1720</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08601.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1720"/>
    <dbReference type="EnsemblBacteria" id="ABF08601">
        <property type="protein sequence ID" value="ABF08601"/>
        <property type="gene ID" value="Rmet_1720"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1720"/>
    <dbReference type="eggNOG" id="COG1414">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_062618_4_0_4"/>
    <dbReference type="OMA" id="CLHTEEG"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029016">
        <property type="entry name" value="GAF-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005471">
        <property type="entry name" value="Tscrpt_reg_IclR_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09339">
        <property type="entry name" value="HTH_IclR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00346">
        <property type="entry name" value="HTH_ICLR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51077">
        <property type="entry name" value="HTH_ICLR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH iclR-type" evidence="3">
        <location>
            <begin position="74"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF08601.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="310" mass="33332" checksum="AE9A25A4A46DE305" modified="2006-05-30" version="1">MVGDRLASDGACWRDGLYEVNPLQSTLHRWTTTTGVLVHNVHNLGINPATYSRPAGFLMPSSAKADAPLPQPGVQSLARAFALLHLLGTHHDPGLTLPELATLANIDRTTAHRMIRFLETAGYAEREPAGKRYHLGSAAMALGLRAMNRPPPDSRIVTRMKAVARLTGDAVFLIGRLGDHGHCLHTEEGAHRIKTFDLLTGTSRLLGQGTASMALMARLDNDEIRKHYNRHRAEYEQGGLSLLALLRGVDRSRKLGYVLAGAGGVAGVGYLLPLALEGDLAVSVVSAASRMPVSRRHEIGALLQATFGAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LMR8</accession>
    <name>Q1LMR8_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Universal stress protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">uspA5</name>
        <name evidence="4" type="ordered locus">Rmet_1677</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the universal stress protein A family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08558.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516406.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1677"/>
    <dbReference type="EnsemblBacteria" id="ABF08558">
        <property type="protein sequence ID" value="ABF08558"/>
        <property type="gene ID" value="Rmet_1677"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1677"/>
    <dbReference type="eggNOG" id="COG0589">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_049301_11_0_4"/>
    <dbReference type="OMA" id="ANDWEAD"/>
    <dbReference type="OrthoDB" id="2040681at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006015">
        <property type="entry name" value="Universal_stress_UspA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006016">
        <property type="entry name" value="UspA"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00582">
        <property type="entry name" value="Usp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006276">
        <property type="entry name" value="UspA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01438">
        <property type="entry name" value="UNVRSLSTRESS"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Usp" evidence="3">
        <location>
            <begin position="1"/>
            <end position="141"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008791"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRNR" id="PIRNR006276"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF08558.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="146" mass="15453" checksum="206F4333DBB8FC9E" modified="2006-05-30" version="1">MYKKILLAVDDSDAAELALAQAVTVAKAMSAEVKALFVVTQALGSTLTPAEAATAAIGKRILEHVRSKMEANGLHYSTELLEEAGSAAGQIADTITSEANDWEADLIVMGTHGRRGIKRMVLGSVSEGVLTQSRIPVLVVRSDFED</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LNG8</accession>
    <name>Q1LNG8_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_1425</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08308.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516176.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1425"/>
    <dbReference type="EnsemblBacteria" id="ABF08308">
        <property type="protein sequence ID" value="ABF08308"/>
        <property type="gene ID" value="Rmet_1425"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1425"/>
    <dbReference type="eggNOG" id="COG1633">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_076834_0_0_4"/>
    <dbReference type="OMA" id="ITQHVFF"/>
    <dbReference type="OrthoDB" id="644190at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.620.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009078">
        <property type="entry name" value="Ferritin-like_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012348">
        <property type="entry name" value="RNR-like"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47240">
        <property type="entry name" value="SSF47240"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF08308.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="279" mass="32541" checksum="EFFA73A0F890B1BB" modified="2006-05-30" version="1">MTATHPAKAPGDLPWSISEIDYSSIDVVKVRNNRTLFYLLAAASFIESGSDTYAGNLAKYYADVPEAARWLAEHWECEELQHGQALRRYVEHVWPEFNWERGYRRFFDEYSVTCSIDNFEPSLALEMAARCVVETGTATYYRALEQASDEPVLRDLTRRIANDEVRHYKHFYRYFNALHEHERLGRGKVLGALARRLLEIKNEDAEIALRNVLRIEHEREDVPEGDVRALNSRCSAVIRHHLPLEMTVRMLLRPLHLNARVERFLRRPIVAVVSRVMLY</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-01-15" modified="2022-08-03" version="95" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LNH0</accession>
    <name>DAPD_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase</fullName>
            <ecNumber evidence="1">2.3.1.117</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Tetrahydrodipicolinate N-succinyltransferase</fullName>
            <shortName evidence="1">THDP succinyltransferase</shortName>
            <shortName evidence="1">THP succinyltransferase</shortName>
            <shortName evidence="1">Tetrahydropicolinate succinylase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">dapD</name>
        <name type="ordered locus">Rmet_1423</name>
    </gene>
    <organism>
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(S)-2,3,4,5-tetrahydrodipicolinate + H2O + succinyl-CoA = (S)-2-succinylamino-6-oxoheptanedioate + CoA</text>
            <dbReference type="Rhea" id="RHEA:17325"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15685"/>
            <dbReference type="ChEBI" id="CHEBI:16845"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57292"/>
            <dbReference type="EC" id="2.3.1.117"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-lysine biosynthesis via DAP pathway; LL-2,6-diaminopimelate from (S)-tetrahydrodipicolinate (succinylase route): step 1/3.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homotrimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the transferase hexapeptide repeat family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.117" evidence="1"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08306.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008649080.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q1LNH0"/>
    <dbReference type="SMR" id="Q1LNH0"/>
    <dbReference type="STRING" id="266264.Rmet_1423"/>
    <dbReference type="EnsemblBacteria" id="ABF08306">
        <property type="protein sequence ID" value="ABF08306"/>
        <property type="gene ID" value="Rmet_1423"/>
    </dbReference>
    <dbReference type="GeneID" id="60822180"/>
    <dbReference type="KEGG" id="rme:Rmet_1423"/>
    <dbReference type="eggNOG" id="COG2171">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_050859_0_1_4"/>
    <dbReference type="OMA" id="YFPIQKM"/>
    <dbReference type="OrthoDB" id="752123at2"/>
    <dbReference type="UniPathway" id="UPA00034">
        <property type="reaction ID" value="UER00019"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008666">
        <property type="term" value="F:2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019877">
        <property type="term" value="P:diaminopimelate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009089">
        <property type="term" value="P:lysine biosynthetic process via diaminopimelate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.166.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00811">
        <property type="entry name" value="DapD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005664">
        <property type="entry name" value="DapD_Trfase_Hexpep_rpt_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001451">
        <property type="entry name" value="Hexapep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018357">
        <property type="entry name" value="Hexapep_transf_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023180">
        <property type="entry name" value="THP_succinylTrfase_dom1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037133">
        <property type="entry name" value="THP_succinylTrfase_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011004">
        <property type="entry name" value="Trimer_LpxA-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00132">
        <property type="entry name" value="Hexapep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14602">
        <property type="entry name" value="Hexapep_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14805">
        <property type="entry name" value="THDPS_N_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51161">
        <property type="entry name" value="SSF51161"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00965">
        <property type="entry name" value="dapD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00101">
        <property type="entry name" value="HEXAPEP_TRANSFERASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0012">Acyltransferase</keyword>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0220">Diaminopimelate biosynthesis</keyword>
    <keyword id="KW-0457">Lysine biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0677">Repeat</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_1000047166" description="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase">
        <location>
            <begin position="1"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="106"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="143"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00811"/>
        </source>
    </evidence>
    <sequence length="274" mass="29525" checksum="0D09E9517A1E26D7" modified="2006-05-30" version="1">MTQALQALIDQAWEDRTSLSPKSAPNDIREAVANVISQLDSGALRVAEKQGKDWVVNQWIKKAVLLSFRLEDNAPMSAGGFAQFYDKVPTKFANWTGDDFAKAGFRVVPPAVARRGSYIARNAVLMPSYVNIGAYVDEGTMVDTWATVGSCAQIGKNVHLSGGVGIGGVLEPLQANPVIIEDNCFIGARSEVVEGVIIEENSVISMGVYLGQSTKIYDRETGEIHYGRVPAGSVVVAGNLPSKDGKYSLYCAVIVKKVDAQTRAKTSLNDLLRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LNL7</accession>
    <name>Q1LNL7_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Sulfate transport system permease protein CysT</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">cysU</name>
        <name evidence="11" type="ordered locus">Rmet_1376</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Part of the ABC transporter complex (TC 3.A.1.6.1) involved in sulfate/thiosulfate import.</text>
    </comment>
    <comment type="function">
        <text evidence="8">Part of the ABC transporter complex CysAWTP (TC 3.A.1.6.1) involved in sulfate/thiosulfate import. Probably responsible for the translocation of the substrate across the membrane.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">The complex is composed of two ATP-binding proteins (CysA), two transmembrane proteins (CysT and CysW) and a solute-binding protein (CysP).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cell inner membrane</location>
            <topology evidence="9">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the binding-protein-dependent transport system permease family. CysTW subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08259.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1376"/>
    <dbReference type="EnsemblBacteria" id="ABF08259">
        <property type="protein sequence ID" value="ABF08259"/>
        <property type="gene ID" value="Rmet_1376"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1376"/>
    <dbReference type="eggNOG" id="COG0555">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016047_14_0_4"/>
    <dbReference type="OMA" id="VFISGNM"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015419">
        <property type="term" value="F:ABC-type sulfate transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011865">
        <property type="entry name" value="CysT_permease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005667">
        <property type="entry name" value="Sulph_transpt2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30406">
        <property type="entry name" value="PTHR30406"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30406:SF8">
        <property type="entry name" value="PTHR30406:SF8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="SSF161098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00969">
        <property type="entry name" value="3a0106s02"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02139">
        <property type="entry name" value="permease_CysT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="9" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9" id="KW-0764">Sulfate transport</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 9" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="12"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="56"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="90"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="124"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="180"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="234"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="10">
        <location>
            <begin position="52"/>
            <end position="255"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011779"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00025323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU366001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ABF08259.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="282" mass="30409" checksum="02CC6243593452AA" modified="2006-05-30" version="1">MLPGFGLSLGFTIFYLTLIVLIPLSATFLKTFTMTWDAFWTTVSNPRVVASLELSFGASLVAAIINTVFGLVVAWVLVRYRFFGKRVIDALVDLPFALPTAVAGIALTALFAGNGWIGRWLEPLGIKVAFTPLGVVVALTFIGLPFVVRTVQPVLEDVERELEEAAASLGASRLQTFSRVILPAILPALLTGFALSFARATGEYGSVVFISGNMPMVSEIAPLMIYSKLEQYDYAGATAVAVVMLVISFVLLLLINLLQAWTRRHQSGVRAAALPDGNPEVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LNQ4</accession>
    <name>Q1LNQ4_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Conjugal transfer protein TraG (CMGI-2)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">traG</name>
        <name evidence="4" type="ordered locus">Rmet_1339</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the VirD4/TraG family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08222.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1339"/>
    <dbReference type="EnsemblBacteria" id="ABF08222">
        <property type="protein sequence ID" value="ABF08222"/>
        <property type="gene ID" value="Rmet_1339"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1339"/>
    <dbReference type="eggNOG" id="COG3505">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012039_0_1_4"/>
    <dbReference type="OMA" id="RATTYGS"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003688">
        <property type="entry name" value="TraG/VirD4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02534">
        <property type="entry name" value="T4SS-DNA_transf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="572"/>
            <end position="614"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF08222.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="664" mass="72671" checksum="0689592F4301EEC7" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="58" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LNQ6</accession>
    <name>Q1LNQ6_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Lipoprotein (Transfer module CMGI-2)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_1337</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08220.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516102.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1337"/>
    <dbReference type="EnsemblBacteria" id="ABF08220">
        <property type="protein sequence ID" value="ABF08220"/>
        <property type="gene ID" value="Rmet_1337"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1337"/>
    <dbReference type="eggNOG" id="ENOG5031P3X">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_177567_0_0_4"/>
    <dbReference type="OMA" id="CKTERPR"/>
    <dbReference type="OrthoDB" id="1888561at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF08220.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="92" mass="10312" checksum="62C31BC6173857A5" modified="2006-05-30" version="1">MKKIIPFLLVAALTACGQSETPLNATNSKARIPTVEELAANPEHLKELRLQCKTDRPKLGDTICDRVAEATRKRFYGDRDTPYTPPKQSPKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LNZ5</accession>
    <name>Q1LNZ5_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Nuclease ParB (CMGI-2)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">parB</name>
        <name evidence="4" type="ordered locus">Rmet_1245</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08131.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516017.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1245"/>
    <dbReference type="EnsemblBacteria" id="ABF08131">
        <property type="protein sequence ID" value="ABF08131"/>
        <property type="gene ID" value="Rmet_1245"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1245"/>
    <dbReference type="eggNOG" id="COG1475">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019174_1_0_4"/>
    <dbReference type="OMA" id="RNVRTTP"/>
    <dbReference type="OrthoDB" id="1375763at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003115">
        <property type="entry name" value="ParB/Sulfiredoxin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036086">
        <property type="entry name" value="ParB/Sulfiredoxin_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02195">
        <property type="entry name" value="ParBc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00470">
        <property type="entry name" value="ParB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110849">
        <property type="entry name" value="SSF110849"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ParB" evidence="3">
        <location>
            <begin position="28"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="422"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="642"/>
            <end position="683"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="325"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="422"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="661"/>
            <end position="675"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00470"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF08131.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="683" mass="74485" checksum="2349E7AF0E7A5643" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LP12</accession>
    <name>Q1LP12_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Putative branched-chain amino acid ABC transporter, permease protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Rmet_1228</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08114.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516002.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1228"/>
    <dbReference type="EnsemblBacteria" id="ABF08114">
        <property type="protein sequence ID" value="ABF08114"/>
        <property type="gene ID" value="Rmet_1228"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1228"/>
    <dbReference type="eggNOG" id="COG4177">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031365_0_1_4"/>
    <dbReference type="OMA" id="EIASWHQ"/>
    <dbReference type="OrthoDB" id="1456257at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015658">
        <property type="term" value="F:branched-chain amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06581">
        <property type="entry name" value="TM_PBP1_LivM_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001851">
        <property type="entry name" value="ABC_transp_permease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043428">
        <property type="entry name" value="LivM-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30482">
        <property type="entry name" value="PTHR30482"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02653">
        <property type="entry name" value="BPD_transp_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="17"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="100"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="128"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="181"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="228"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="269"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="305"/>
            <end position="329"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABF08114.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="354" mass="38092" checksum="B30B947B088D1F88" modified="2006-05-30" version="1">MHSDSLTPPPQDIRRPAWMATAGWLVALAVLCAMPMLLTADSHKYYIELLSKVMIMAIFALSLQLLIGYTGLVSLGHAAYFAMAAYATAMLAPESGPGNGWLLLFGAVGAAAALALVVGALVLRTRGIYFIMVTLAFAQMVYFVFHDTKIAGGSDGTYIYFRPEFGLFSARPVTVNDPVQFYWLVLVALALTVAALTLLLRSRFGHALVGIRHNEQRMRAAGFGTYRYQLGAFVVGGAFAGLAGYLYAIQFGFVNPEIASWHQSGNAMLMVILGGVGSLAGAVLGAFSFVLLAEWFGTLTKHWQLLMGGFIILAVALLPRGLVSVPAVLRHRSPRRARRAGAATESNTRSREAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="64" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LPE5</accession>
    <name>Q1LPE5_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Omp85 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Rmet_1095</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell outer membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07981.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515882.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1095"/>
    <dbReference type="EnsemblBacteria" id="ABF07981">
        <property type="protein sequence ID" value="ABF07981"/>
        <property type="gene ID" value="Rmet_1095"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1095"/>
    <dbReference type="eggNOG" id="COG0729">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046092_1_0_4"/>
    <dbReference type="OMA" id="YRFEFKP"/>
    <dbReference type="OrthoDB" id="364966at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009279">
        <property type="term" value="C:cell outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000184">
        <property type="entry name" value="Bac_surfAg_D15"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039910">
        <property type="entry name" value="D15-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12815">
        <property type="entry name" value="PTHR12815"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01103">
        <property type="entry name" value="Omp85"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1134">Transmembrane beta strand</keyword>
    <feature type="domain" description="Omp85" evidence="4">
        <location>
            <begin position="108"/>
            <end position="320"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004442"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABF07981.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="386" mass="42679" checksum="B2077F7BF039F7BC" modified="2006-05-30" version="1">MRSHPQTICILVIGLWCATAISAEVNQPDDSEKQGWIDRLLNKLGASKEVDVSKGIDWGVLPGPFYNPEMGFGIGAAAIGLYKPKSAEAETQLSTLSIRGFGTVKGAYGVSVENNTFFADDSYRVTIEADLINMPTAYWGIGYSNAIDSANKEAYTRQTVALQPKILFRVAPNVYIGAGYSLQYDNATKLERGAESALATDPHGPRVLSSGISAHFSYDTRDFIPNPYRGESLLANATVYRRALGSDTDMENLEITANKYFRMRERDVLAFDLYGNFNFGDVPWDMMATLGNNKRMRGYFLGQFRDKVMAGVQAEYRWHIVGRQGMVFWVGTGAVAPNPGALTSAHWLPNAGVGYRFEFKPRVNVRFDAGVGRNTKGVYFQINEAF</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LPK3</accession>
    <name>Q1LPK3_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Putative peptidase, M23B subfamily</fullName>
            <ecNumber evidence="4">3.4.24.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">nlpD</name>
        <name evidence="4" type="ordered locus">Rmet_1037</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EC" id="3.4.24.-" evidence="4"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07923.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515828.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1037"/>
    <dbReference type="EnsemblBacteria" id="ABF07923">
        <property type="protein sequence ID" value="ABF07923"/>
        <property type="gene ID" value="Rmet_1037"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1037"/>
    <dbReference type="eggNOG" id="COG1388">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG4942">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029425_0_4_4"/>
    <dbReference type="OMA" id="YYRVERG"/>
    <dbReference type="OrthoDB" id="1891666at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00118">
        <property type="entry name" value="LysM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.70.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.350.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011055">
        <property type="entry name" value="Dup_hybrid_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018392">
        <property type="entry name" value="LysM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036779">
        <property type="entry name" value="LysM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016047">
        <property type="entry name" value="Peptidase_M23"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01476">
        <property type="entry name" value="LysM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01551">
        <property type="entry name" value="Peptidase_M23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00257">
        <property type="entry name" value="LysM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51261">
        <property type="entry name" value="SSF51261"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51782">
        <property type="entry name" value="LYSM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193680" evidence="2">
        <location>
            <begin position="33"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="domain" description="LysM" evidence="3">
        <location>
            <begin position="45"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="90"/>
            <end position="129"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF07923.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="240" mass="25673" checksum="D0A875D6EA99B7FF" modified="2006-05-30" version="1">MNRTHHANLQTRRRALGALGSLAAISLSALLAACGTTPTEPAPDGFYRVERGDTLYSIARRNKRSVSELARWNNMSNTNQIEVGQLIRVRPPGGASTSAATTPQSDKPARPVQDMPSAKPPASSISLQWPADGRVSGRFAPPAEKGMRIEVPAGGKVRAAAPGWAIHVGTLRGYGMLVIVKHNDDWLTVYGNLDKPLVKEGDRVTAGQEIGSMAQAAELHFEVRGNGRPLNPANYLPSRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LPM7</accession>
    <name>Q1LPM7_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Transcriptional regulator, TetR family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_1013</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07899.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008649315.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1013"/>
    <dbReference type="EnsemblBacteria" id="ABF07899">
        <property type="protein sequence ID" value="ABF07899"/>
        <property type="gene ID" value="Rmet_1013"/>
    </dbReference>
    <dbReference type="GeneID" id="60821874"/>
    <dbReference type="KEGG" id="rme:Rmet_1013"/>
    <dbReference type="eggNOG" id="COG1309">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069356_13_2_4"/>
    <dbReference type="OMA" id="WMPNFID"/>
    <dbReference type="OrthoDB" id="1437290at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001647">
        <property type="entry name" value="HTH_TetR"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00440">
        <property type="entry name" value="TetR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00455">
        <property type="entry name" value="HTHTETR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50977">
        <property type="entry name" value="HTH_TETR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH tetR-type" evidence="2">
        <location>
            <begin position="24"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="H-T-H motif" evidence="1">
        <location>
            <begin position="47"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF07899.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="209" mass="22707" checksum="AA66698D2C054117" modified="2006-05-30" version="1">MAPMTTELSPARRYRGAEAEERRAQRRGQLIAAAVQVYGERGYQNATVKAVCEAAGLTERYFYESFANSEALLLASFQTVTDLLLQKIELAGEAAGGDGEHRALAILRAYFGTLQCEPRSARVFLVEIRGVSKLVDEALGLSLRDFGGLLARVLMPEGKEPDPMLLAGVAGGIIHVALHWIRHGYAPDVDTVAATALQLALVLKDRQRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="112" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LPP1</accession>
    <name>Q1LPP1_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">NAD kinase</fullName>
            <ecNumber evidence="5">2.7.1.23</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">ATP-dependent NAD kinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5 6" type="primary">nadK</name>
        <name evidence="6" type="ordered locus">Rmet_0999</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Involved in the regulation of the intracellular balance of NAD and NADP, and is a key enzyme in the biosynthesis of NADP. Catalyzes specifically the phosphorylation on 2'-hydroxyl of the adenosine moiety of NAD to yield NADP.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>ATP + NAD(+) = ADP + H(+) + NADP(+)</text>
            <dbReference type="Rhea" id="RHEA:18629"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.23"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the NAD kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.23" evidence="5"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07885.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0999"/>
    <dbReference type="EnsemblBacteria" id="ABF07885">
        <property type="protein sequence ID" value="ABF07885"/>
        <property type="gene ID" value="Rmet_0999"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0999"/>
    <dbReference type="eggNOG" id="COG0061">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008831_0_1_4"/>
    <dbReference type="OMA" id="VNLGHVG"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003951">
        <property type="term" value="F:NAD+ kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019674">
        <property type="term" value="P:NAD metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006741">
        <property type="term" value="P:NADP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.200.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00361">
        <property type="entry name" value="NAD_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017438">
        <property type="entry name" value="ATP-NAD_kinase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017437">
        <property type="entry name" value="ATP-NAD_kinase_PpnK-typ_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016064">
        <property type="entry name" value="NAD/diacylglycerol_kinase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002504">
        <property type="entry name" value="NADK"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01513">
        <property type="entry name" value="NAD_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111331">
        <property type="entry name" value="SSF111331"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 5 6" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 5" id="KW-0520">NAD</keyword>
    <keyword evidence="3 5" id="KW-0521">NADP</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 5 6" id="KW-0808">Transferase</keyword>
    <feature type="active site" description="Proton acceptor" evidence="5">
        <location>
            <position position="90"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="90"/>
            <end position="91"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="164"/>
            <end position="165"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="192"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="194"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="229"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="263"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABF07885.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="313" mass="33533" checksum="A4BEF519E3A32DD5" modified="2006-05-30" version="1">MPVWSNPGMSAPHKTGAARPPFKTVALVGRHSTAGIEGPLEELASCISRNGQDVVFERETAMATGLTGYPVLTPDEIGREADVAVVLGGDGTLLGLARQLAGHDVPVIGVNHGRLGFMTDIPLDAISTALPEMLSGRYEAETRMLLESRVIRDDSVIFSALAFNDVVVNRSGISGMVELAVSVDGYFMYNQRSDGLIVSTPTGSTAYALAAGGPILHPTLSGLVLVPIAPHALSNRPIVLPYDAEVTIEVASARDVSVNFDMQALASLLPGDRIVVRRSAKTINLLHPVGYNYYATLRKKLHWHEYPTEDNRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LPR6</accession>
    <name>Q1LPR6_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">3-hydroxybutyryl-CoA dehydrogenase</fullName>
            <ecNumber evidence="4">1.1.1.157</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">paaH</name>
        <name evidence="4" type="ordered locus">Rmet_0974</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EC" id="1.1.1.157" evidence="4"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07860.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515778.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0974"/>
    <dbReference type="EnsemblBacteria" id="ABF07860">
        <property type="protein sequence ID" value="ABF07860"/>
        <property type="gene ID" value="Rmet_0974"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0974"/>
    <dbReference type="eggNOG" id="COG1250">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009834_2_3_4"/>
    <dbReference type="OMA" id="HPVRLYD"/>
    <dbReference type="OrthoDB" id="862072at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008691">
        <property type="term" value="F:3-hydroxybutyryl-CoA dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070403">
        <property type="term" value="F:NAD+ binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044248">
        <property type="term" value="P:cellular catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006631">
        <property type="term" value="P:fatty acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901575">
        <property type="term" value="P:organic substance catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1040.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006176">
        <property type="entry name" value="3-OHacyl-CoA_DH_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006108">
        <property type="entry name" value="3HC_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008927">
        <property type="entry name" value="6-PGluconate_DH-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013328">
        <property type="entry name" value="6PGD_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00725">
        <property type="entry name" value="3HCDH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02737">
        <property type="entry name" value="3HCDH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48179">
        <property type="entry name" value="SSF48179"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0520">NAD</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="3HCDH_N" evidence="3">
        <location>
            <begin position="9"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="domain" description="3HCDH" evidence="2">
        <location>
            <begin position="189"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="domain" description="3HCDH" evidence="2">
        <location>
            <begin position="416"/>
            <end position="495"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF02737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF07860.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="507" mass="53743" checksum="094990A09425E6F8" modified="2006-05-30" version="1">MSNSTIQTLGIVGTGAMGRGIAQIAAQAGLTVNLFDTNAQAVAAARQYLQDTFTKLAEKGKMSAADGEAALARVKACAALEDLTACDMVLEAIVERLDVKRDLVAKLEAVVREDAVIASNTSSLSITAIAVGAKKPGRVVGYHFFNPVPLMKVVEVVDGISGDPAVGDRLMELSRRMGHTPVRCKDMPGFIVNHAGRGMNIEGLKVAQEGVAEFVDIDNIMREQAGFRMGPFELMDLTGLDVSHPVMESIYNQFYQEPRYRPSPITSLRSVGGLLGRKTGAGFYAYPNGQKQVPAAAPVPDARPASVWVSRANARGHAMTVKLLTALGVTVEAGNQPPADALIVVTPLGLDATSAALEEGLDATRVVAIDTLMPFEATKRRTLMTTPATSAAARDAAHGLFASDGVPVTVIRDSAGFVAQRVISCIVNIASDIAQQRIATPTDIDLAVHLGLGYPKGPLALGDTIGADLILEVLRNLNRLTGDMRYRPSPWLWRRAGLGLSLLTEEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="100" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LPV0</accession>
    <name>Q1LPV0_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">NADH-quinone oxidoreductase subunit N</fullName>
            <ecNumber evidence="5">7.1.1.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">NADH dehydrogenase I subunit N</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="5">NDH-1 subunit N</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5 8" type="primary">nuoN</name>
        <name evidence="8" type="ordered locus">Rmet_0940</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>a quinone + 5 H(+)(in) + NADH = a quinol + 4 H(+)(out) + NAD(+)</text>
            <dbReference type="Rhea" id="RHEA:57888"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:24646"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:132124"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="5">NDH-1 is composed of 14 different subunits. Subunits NuoA, H, J, K, L, M, N constitute the membrane sector of the complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cell inner membrane</location>
            <topology evidence="5">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 6">Membrane</location>
            <topology evidence="1 6">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the complex I subunit 2 family.</text>
    </comment>
    <dbReference type="EC" id="7.1.1.-" evidence="5"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07826.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515753.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0940"/>
    <dbReference type="DNASU" id="4037737"/>
    <dbReference type="EnsemblBacteria" id="ABF07826">
        <property type="protein sequence ID" value="ABF07826"/>
        <property type="gene ID" value="Rmet_0940"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0940"/>
    <dbReference type="eggNOG" id="COG1007">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007100_1_3_4"/>
    <dbReference type="OMA" id="FYIRVIV"/>
    <dbReference type="OrthoDB" id="1664448at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008137">
        <property type="term" value="F:NADH dehydrogenase (ubiquinone) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048038">
        <property type="term" value="F:quinone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042773">
        <property type="term" value="P:ATP synthesis coupled electron transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00445">
        <property type="entry name" value="NDH1_NuoN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010096">
        <property type="entry name" value="NADH-Q_OxRdtase_suN/2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001750">
        <property type="entry name" value="ND/Mrp_mem"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00361">
        <property type="entry name" value="Proton_antipo_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01770">
        <property type="entry name" value="NDH_I_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0520">NAD</keyword>
    <keyword evidence="8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-0874">Quinone</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-1278">Translocase</keyword>
    <keyword evidence="2 5 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <keyword evidence="5 8" id="KW-0830">Ubiquinone</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="6"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="37"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="75"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="106"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="129"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="160"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="201"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="240"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="274"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="300"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="335"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="378"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="413"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="465"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="domain" description="Proton_antipo_M" evidence="7">
        <location>
            <begin position="126"/>
            <end position="427"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00445"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ABF07826.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="491" mass="52758" checksum="B9123B94F3DCD28C" modified="2006-05-30" version="1">MQSSSSLAPVAPIIFLAIAIAAINWIDLARGKNRTSVAYPLSLLTTLVLTAWFGIDAAGGETHYAFANLVVIDPMANVLAAFCSLGMLLTLIYTRSYLAERDMFAGEFYMLALFTLGGQIVMITGNNFLTLYLGLELLSLSSYALVALRRDSRVTSESAIKYFVLGALASGFLLYGMSMMYGATGSLNLGEVFRVVESGRVNTTMLAFGVVFIVAGVAFKLGAAPFHMWIPDIYQGSPTAVTLLIAAGPKVAAFALIIRLLVEGLLPLATDWQVMLVILSIASLVIGNLTAIVQTNLKRMLAYSTISHMGFVLLGMLSGVVANKADGAVNAYSSSMFYSITYMLTTLGTFGLILVRTRKGFEAETLDDLKGMSRRHPWFAFLMLVMMFSMAGIPPTVGFYAKLSVLQAVVSAGMSWLAVLAVVFSLIGAFYYLRIVKLMYFDAPVGEEPLEASTGLRSVLSLNGLAVILLGLFPAGLMDLCFQAIRATLAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="105" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LQA6</accession>
    <name>Q1LQA6_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 7">Phosphoribosylamine--glycine ligase</fullName>
            <ecNumber evidence="2 7">6.3.4.13</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">GARS</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="7">Glycinamide ribonucleotide synthetase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="7">Phosphoribosylglycinamide synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7 10" type="primary">purD</name>
        <name evidence="10" type="ordered locus">Rmet_0784</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>5-phospho-beta-D-ribosylamine + ATP + glycine = ADP + H(+) + N(1)-(5-phospho-beta-D-ribosyl)glycinamide + phosphate</text>
            <dbReference type="Rhea" id="RHEA:17453"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
            <dbReference type="ChEBI" id="CHEBI:58681"/>
            <dbReference type="ChEBI" id="CHEBI:143788"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.4.13"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 7">Purine metabolism; IMP biosynthesis via de novo pathway; N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate: step 2/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the GARS family.</text>
    </comment>
    <dbReference type="EC" id="6.3.4.13" evidence="2 7"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07670.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515624.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0784"/>
    <dbReference type="EnsemblBacteria" id="ABF07670">
        <property type="protein sequence ID" value="ABF07670"/>
        <property type="gene ID" value="Rmet_0784"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0784"/>
    <dbReference type="eggNOG" id="COG0151">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027420_3_1_4"/>
    <dbReference type="OMA" id="KATVCKY"/>
    <dbReference type="OrthoDB" id="932854at2"/>
    <dbReference type="UniPathway" id="UPA00074">
        <property type="reaction ID" value="UER00125"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004637">
        <property type="term" value="F:phosphoribosylamine-glycine ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009113">
        <property type="term" value="P:purine nucleobase biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.600.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00138">
        <property type="entry name" value="GARS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011761">
        <property type="entry name" value="ATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013815">
        <property type="entry name" value="ATP_grasp_subdomain_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016185">
        <property type="entry name" value="PreATP-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020561">
        <property type="entry name" value="PRibGlycinamid_synth_ATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000115">
        <property type="entry name" value="PRibGlycinamide_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020560">
        <property type="entry name" value="PRibGlycinamide_synth_C-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037123">
        <property type="entry name" value="PRibGlycinamide_synth_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020562">
        <property type="entry name" value="PRibGlycinamide_synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011054">
        <property type="entry name" value="Rudment_hybrid_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43472">
        <property type="entry name" value="PTHR43472"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01071">
        <property type="entry name" value="GARS_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02843">
        <property type="entry name" value="GARS_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02844">
        <property type="entry name" value="GARS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01210">
        <property type="entry name" value="GARS_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51246">
        <property type="entry name" value="SSF51246"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52440">
        <property type="entry name" value="SSF52440"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00877">
        <property type="entry name" value="purD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50975">
        <property type="entry name" value="ATP_GRASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 7 10" id="KW-0436">Ligase</keyword>
    <keyword evidence="4 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5 7" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ATP-grasp" evidence="9">
        <location>
            <begin position="107"/>
            <end position="314"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ABF07670.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="422" mass="44714" checksum="AB38D739E9458F47" modified="2006-05-30" version="1">MKVLVVGSGGREHALAWKLAQSPKVQVVYVAPGNGGTALDKRLQNVPLTDPEVLAAFVEREGVAFTVVGPEAPLAGGIVDVFRARGLRIFGPTKAAAQLESSKDFAKAFMHRHGIPTAYYQTFSDAAGAHAYIDAQGAPIVIKADGLAAGKGVVVAMTLEEAHAAVDMMLADNKLGDAGARVVIEEFLEGEEASFIVMVDGKNVLALATSQDHKRLLDGDAGPNTGGMGAYSPAPVVTPALHARALREIILPTVRGMEKDGIPFTGFLYAGLMIDANGNPKTLEFNCRMGDPETQPIMARLKTDLVDVMEAAVDGKLDQIELDWDRRTALGVVMAAYDYPENPRKGDAITGIPAETADAVTFHAGTTLKDGTLLTSGGRVLCVVGLADTVKAAQKNAYAAVEQIRFDGMQFRTDIGYRAIKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="58" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LQE6</accession>
    <name>Q1LQE6_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Putative membrane protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Rmet_0744</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07630.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515585.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0744"/>
    <dbReference type="EnsemblBacteria" id="ABF07630">
        <property type="protein sequence ID" value="ABF07630"/>
        <property type="gene ID" value="Rmet_0744"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0744"/>
    <dbReference type="eggNOG" id="COG2314">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_119469_0_0_4"/>
    <dbReference type="OMA" id="PTRSGWP"/>
    <dbReference type="OrthoDB" id="1471089at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007829">
        <property type="entry name" value="TM2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05154">
        <property type="entry name" value="TM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="23"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="49"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="75"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="121"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="domain" description="TM2" evidence="6">
        <location>
            <begin position="18"/>
            <end position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF05154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABF07630.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="163" mass="17096" checksum="CB659EE143626091" modified="2006-05-30" version="1">MPAASQASAIATEARNTRGKSKLATVALAFLLGSLGAHRFYLKGLGDKFGWVHLLCTVAGVLGIASLTLGTGNTALNWVFAVAGGASLISAFLTAIVFGLRPDDKWDAQYNPHGQPTRSGWPVVILIIFSLMIGTGLLMAGLAISFQTFFESQVEAAKALSQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LQL6</accession>
    <name>Q1LQL6_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">General substrate transporter:Major facilitator superfamily MFS_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Rmet_0674</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07560.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515530.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0674"/>
    <dbReference type="EnsemblBacteria" id="ABF07560">
        <property type="protein sequence ID" value="ABF07560"/>
        <property type="gene ID" value="Rmet_0674"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0674"/>
    <dbReference type="eggNOG" id="COG2271">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001265_0_0_4"/>
    <dbReference type="OMA" id="VYYFSMW"/>
    <dbReference type="OrthoDB" id="1345431at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="SSF103473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="23"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="61"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="93"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="118"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="153"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="186"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="251"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="284"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="317"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="342"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="374"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="408"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="domain" description="MFS" evidence="6">
        <location>
            <begin position="27"/>
            <end position="432"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABF07560.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="437" mass="47955" checksum="2FA211EDBACD1D85" modified="2006-05-30" version="1">MTTTAVSSGAQDAGFEDATYRKVSWRLVPFLLLCYVVAYLDRVNVGFAKLQMLNDLKFSETIYGLGAGIFFIGYFIFEVPSNVILHKVGARIWIARIMITWGLISAAMMFVTTPMMFYVLRFLLGVAEAGFFPGIILYLTYWYPASRRGRTTTYFMTAIALSGVIGGPLSGWLMQSFDGHNGWSGWQWMFLVEGIPSILVGLWVLAYLDDRITHAKWLTDDEKALLQRNIANEDQHKEDPPVAQVLSSPRVWLMSAIYFSFVMGLYGVSFWLPTIIKQTGVKGALDVGLLTAIPYGCAVVGMILFARSADRRGERRWHIAIPALAGAVGLVLSVQWHSDTTMAMVALTIATIGILTTLPLFWSLPTSFLAGTGAAAGIALINSLGNLAGFLSPYAVGWLKDMTQSTDSGMYLLAVCMVVGAALTLSVPARMVNQKKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LQR2</accession>
    <name>Q1LQR2_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative sel1-like repeat harboring protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_0628</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07514.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0628"/>
    <dbReference type="EnsemblBacteria" id="ABF07514">
        <property type="protein sequence ID" value="ABF07514"/>
        <property type="gene ID" value="Rmet_0628"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0628"/>
    <dbReference type="eggNOG" id="COG0790">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033886_1_2_4"/>
    <dbReference type="OMA" id="PPSSAMW"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045653">
        <property type="entry name" value="DUF6396"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006597">
        <property type="entry name" value="Sel1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19933">
        <property type="entry name" value="DUF6396"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00671">
        <property type="entry name" value="SEL1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193716" evidence="1">
        <location>
            <begin position="19"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="domain" description="DUF6396" evidence="2">
        <location>
            <begin position="229"/>
            <end position="336"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF19933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF07514.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="435" mass="47745" checksum="14AF3B9BC6577663" modified="2006-05-30" version="1">MRIALAVLLAAFTLTACSKETSMPTVPDLATVRANLAFTCVREADHLPPLDPEADVLFKYARYLQKLEGPKDFDDVARYYRIAAAHGHYKANSNLQKLVSEGMASSPLPQRESVALASQLIDAGVPSGYYDIGYYLNLGYGLKQDKEMALRYFRKAADLGNADAQFYVGDLLAPRDNAPEIARQMRQCATEQGHGEAAGTLGVNLSGKKHFPEAVAAFQKGVIAGDSMSASFLEDGFKAPPPSDGLNYLALPNDPERSRRYELIWRFLIRNDGRNPKVPDIDQIVPLPPAKLPPWDGTFQWQKEQDAAVPLEKPSVELINRLSEAKNLDPATGLPLTTSAKTAQTTTSAAEQKALPLGTKASTGEQCPQRGLWCVRGAASISGSRREFEKGDILPPLTAYDPRPFAWMDSLLGMREQSADVTWELIAYRATRTTW</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="65" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LQR6</accession>
    <name>Q1LQR6_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_0624</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07510.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515481.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0624"/>
    <dbReference type="EnsemblBacteria" id="ABF07510">
        <property type="protein sequence ID" value="ABF07510"/>
        <property type="gene ID" value="Rmet_0624"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0624"/>
    <dbReference type="eggNOG" id="COG3517">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018386_1_0_4"/>
    <dbReference type="OMA" id="QLDVVMH"/>
    <dbReference type="OrthoDB" id="683477at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010269">
        <property type="entry name" value="T6SS_TssC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044032">
        <property type="entry name" value="TssC1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044031">
        <property type="entry name" value="TssC1_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35565">
        <property type="entry name" value="PTHR35565"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05943">
        <property type="entry name" value="VipB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18945">
        <property type="entry name" value="VipB_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03355">
        <property type="entry name" value="VI_chp_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="VipB" evidence="1">
        <location>
            <begin position="92"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="domain" description="VipB_2" evidence="2">
        <location>
            <begin position="399"/>
            <end position="510"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF05943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF18945"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF07510.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="525" mass="59561" checksum="519F3AC05E4175F4" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LR19</accession>
    <name>Q1LR19_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Extra-cytoplasmic Solute Receptor protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">bug</name>
        <name evidence="4" type="ordered locus">Rmet_0521</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Periplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the UPF0065 (bug) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07407.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008645024.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0521"/>
    <dbReference type="EnsemblBacteria" id="ABF07407">
        <property type="protein sequence ID" value="ABF07407"/>
        <property type="gene ID" value="Rmet_0521"/>
    </dbReference>
    <dbReference type="GeneID" id="60824118"/>
    <dbReference type="KEGG" id="rme:Rmet_0521"/>
    <dbReference type="eggNOG" id="COG3181">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045683_0_0_4"/>
    <dbReference type="OMA" id="CRPNDLP"/>
    <dbReference type="OrthoDB" id="447734at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042597">
        <property type="term" value="C:periplasmic space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005064">
        <property type="entry name" value="BUG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042100">
        <property type="entry name" value="Bug_dom1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42928">
        <property type="entry name" value="PTHR42928"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03401">
        <property type="entry name" value="TctC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017082">
        <property type="entry name" value="YflP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0675">Receptor</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193723" evidence="3">
        <location>
            <begin position="25"/>
            <end position="326"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF07407.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="326" mass="34287" checksum="9D44C3CA1A3DAB77" modified="2006-05-30" version="1">MQARKTALIAAVAAAASVASVAHAQSNYPTKPIRLIIPFAPGGTTDIVGRGVADQMSRILGQTVVVENRAGGGGSIGADSIAKAAPDGYTIGISTVSTMAVNPACNPKLSYDPIKDFKPIANVANVANVIAVNPNFPAKDYKEFLAVLKANPGKYSYASSGTCGFGHMLGEQFKVSTKTFMVHIPYRGAGPALNDVLAGQVPIMVDNLPSSMPYIKAGKLRPIVVAWNKRVEGLPDVPTFGEMGLKEPNDPAWYGLVAPAGTPDDVINKLNAAVVKALQDKAFVQRLQAAGAEPSGNTPAQHAAEIKKEFDKMKNLVKVQNIKLEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-10-07" version="92" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LR30</accession>
    <name>Q1LR30_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Transcriptional regulator, LysR-family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_0510</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the LysR transcriptional regulatory family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07396.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0510"/>
    <dbReference type="EnsemblBacteria" id="ABF07396">
        <property type="protein sequence ID" value="ABF07396"/>
        <property type="gene ID" value="Rmet_0510"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0510"/>
    <dbReference type="eggNOG" id="COG0583">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039613_35_0_4"/>
    <dbReference type="OMA" id="MWRQEIL"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005119">
        <property type="entry name" value="LysR_subst-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000847">
        <property type="entry name" value="Tscrpt_reg_HTH_LysR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00126">
        <property type="entry name" value="HTH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03466">
        <property type="entry name" value="LysR_substrate"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00039">
        <property type="entry name" value="HTHLYSR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50931">
        <property type="entry name" value="HTH_LYSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH lysR-type" evidence="2">
        <location>
            <begin position="15"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF07396.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="311" mass="34901" checksum="F0CCF64F19C51C82" modified="2006-05-30" version="1">MREGRKPTRTHTMSLSLEQLQAFAAAAETGSFSAAARQLGKAQSVVSAAVANLEIDLGNALFDRTARYPVLTPAGERLLVEARVILERCEHFRGVAKSLGEGVESRLTLAVDELYPEETLGVLLDEFSQRFPSVELELLFPLMEDVSRLVLSQCADLGIMWRQEILPPELGFHALGWVPMPIICAPDHPLAAQPRVDFEELKRYRQLMVATRSDSEEKKRLRVAAEVWWVESQWVIVELVKRKLGWAFVPWHVAANSPAAAGLVSPQLTFQDQDWPVAMEIVWHKQRPLGKAATWLRERVSQQPLPMPGGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LRI3</accession>
    <name>Q1LRI3_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 9">Trehalose-6-phosphate synthase</fullName>
            <ecNumber evidence="5 9">2.4.1.15</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Osmoregulatory trehalose synthesis protein A</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">UDP-glucose-glucosephosphate glucosyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="primary">otsA</name>
        <name evidence="10" type="ordered locus">Rmet_0357</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Probably involved in the osmoprotection via the biosynthesis of trehalose. Catalyzes the transfer of glucose from UDP-alpha-D-glucose (UDP-Glc) to D-glucose 6-phosphate (Glc-6-P) to form trehalose-6-phosphate. Acts with retention of the anomeric configuration of the UDP-sugar donor.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 9">
            <text>D-glucose 6-phosphate + UDP-alpha-D-glucose = alpha,alpha-trehalose 6-phosphate + H(+) + UDP</text>
            <dbReference type="Rhea" id="RHEA:18889"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:58429"/>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
            <dbReference type="ChEBI" id="CHEBI:61548"/>
            <dbReference type="EC" id="2.4.1.15"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 9">Glycan biosynthesis; trehalose biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4 9">Homotetramer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the glycosyltransferase 20 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.15" evidence="5 9"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07243.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515236.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0357"/>
    <dbReference type="CAZy" id="GT20">
        <property type="family name" value="Glycosyltransferase Family 20"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF07243">
        <property type="protein sequence ID" value="ABF07243"/>
        <property type="gene ID" value="Rmet_0357"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0357"/>
    <dbReference type="eggNOG" id="COG0380">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002351_7_1_4"/>
    <dbReference type="OMA" id="HFSHTPW"/>
    <dbReference type="OrthoDB" id="556566at2"/>
    <dbReference type="UniPathway" id="UPA00299"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003825">
        <property type="term" value="F:alpha,alpha-trehalose-phosphate synthase (UDP-forming) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005992">
        <property type="term" value="P:trehalose biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd03788">
        <property type="entry name" value="GT20_TPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001830">
        <property type="entry name" value="Glyco_trans_20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012766">
        <property type="entry name" value="Trehalose_OtsA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10788">
        <property type="entry name" value="PTHR10788"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00982">
        <property type="entry name" value="Glyco_transf_20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02400">
        <property type="entry name" value="trehalose_OtsA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 9 10" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 9 10" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008799"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00018539"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU362045"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ABF07243.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="464" mass="52226" checksum="FC9A02B6BAEF2EE6" modified="2006-05-30" version="1">MGRLVAVSNRVADPRNHAAGGLAVALSDTLNKTGGLWFGWSGKVVDASRGGTPGEGELHQIQAGNVTLATVDLSREDFESYYLGYSNGVLWPVFHYRVDLADFDSGGNLNAYRRVNQLFARKLAPLLKPDDVIWIHDYHLIPLAAELRAIGCGQRIGFFLHIPLPPRLILATIPQHEWLMRALFAYDLLGFQSHDDVEHFSRYVQGEAGAEPMGEYRFRAFHRTVRAQAFPIGIDVDEFIELGRADEARETYEMMRGQYARRRLLLGIDRLDYSKGLPQRLKAFQTLLAEYPENRSSATLIQIAAPSRESVDAYADLRRDMEGITGAINGEYGELDWMPVRYIHRSTARRRLPGLCRACNVALVTPLRDGMNLVAKEYIAAQDPEDPGVLVLSRFAGAAEQLKEALLVNPYDTRATAQAIQQALHMSLSERQARHQKLLERIRQQDVHWWSGEYLRALMETEGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="99" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LRN6</accession>
    <name>Q1LRN6_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Lipopolysaccharide export system ATP-binding protein LptB</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">lptB</name>
        <name evidence="16" type="ordered locus">Rmet_0304</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Part of the ABC transporter complex LptBFG involved in the translocation of lipopolysaccharide (LPS) from the inner membrane to the outer membrane. Probably responsible for energy coupling to the transport system.</text>
    </comment>
    <comment type="subunit">
        <text evidence="13">Component of the lipopolysaccharide transport and assembly complex. The LptBFG transporter is composed of two ATP-binding proteins (LptB) and two transmembrane proteins (LptF and LptG).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell inner membrane</location>
            <topology evidence="3">Peripheral membrane protein</topology>
            <orientation evidence="3">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the ABC transporter superfamily. Outer membrane lipopolysaccharide export (TC 1.B.42) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07190.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515190.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0304"/>
    <dbReference type="EnsemblBacteria" id="ABF07190">
        <property type="protein sequence ID" value="ABF07190"/>
        <property type="gene ID" value="Rmet_0304"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0304"/>
    <dbReference type="eggNOG" id="COG1137">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_2_4"/>
    <dbReference type="OMA" id="LPMYQRA"/>
    <dbReference type="OrthoDB" id="1220708at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043190">
        <property type="term" value="C:ATP-binding cassette (ABC) transporter complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03218">
        <property type="entry name" value="ABC_YhbG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032823">
        <property type="entry name" value="BCA_ABC_TP_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030921">
        <property type="entry name" value="LPS_export_LptB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12399">
        <property type="entry name" value="BCA_ABC_TP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR04406">
        <property type="entry name" value="LPS_export_lptB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 14 16" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-1278">Translocase</keyword>
    <feature type="domain" description="ABC transporter" evidence="15">
        <location>
            <begin position="22"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <begin position="54"/>
            <end position="61"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004287"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00017803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00024818"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00026081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="ABF07190.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="263" mass="28711" checksum="5427EC3C218D4C8E" modified="2006-05-30" version="1">MNDTATLADQASSAPSTDSSTLVVRHLKKRYGSRTVVKDVSLDVKSGEVVGLLGPNGAGKTTSFYMIVGLVALDEGDIVLDGQHISGLAIHERARMGLSYLPQEASVFRKLNVEENIRAVLELQVVNGKPLPKQEIDRRLDSLLDDLQIAHLRNNPALSLSGGERRRVEIARALASAPRFILLDEPFAGVDPIAVGEIQRIVSFLKARNIGVLITDHNVRETLGICDHAYIISEGTVLAEGQPEEIIANESVRRVYLGENFRM</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="150" xmlns="http://uniprot.org/uniprot">
    <accession>Q44007</accession>
    <accession>Q58AM0</accession>
    <name>CZCS_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName>Sensor protein CzcS</fullName>
            <ecNumber>2.7.13.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">czcS</name>
        <name type="ordered locus">Rmet_5977</name>
    </gene>
    <organism>
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid">
        <name>pMOL30</name>
    </geneLocation>
    <reference key="1">
        <citation type="journal article" date="1997" name="Mol. Microbiol." volume="23" first="493" last="503">
            <title>Two-component regulatory system involved in transcriptional control of heavy-metal homoeostasis in Alcaligenes eutrophus.</title>
            <authorList>
                <person name="van der Lelie D."/>
                <person name="Schwuchow T."/>
                <person name="Schwidetzky T."/>
                <person name="Wuertz S."/>
                <person name="Baeyens W."/>
                <person name="Mergeay M."/>
                <person name="Nies D.H."/>
            </authorList>
            <dbReference type="PubMed" id="9044283"/>
            <dbReference type="DOI" id="10.1046/j.1365-2958.1997.d01-1866.x"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC DNA]</scope>
    </reference>
    <reference key="2">
        <citation type="submission" date="2004-11" db="EMBL/GenBank/DDBJ databases">
            <title>Sequence and features of the Ralstonia metallidurans CH34 heavy metals plasmids pMOL28 and pMOL30.</title>
            <authorList>
                <person name="Monchy S."/>
                <person name="van der Lelie D."/>
                <person name="Vallaeys T."/>
                <person name="Taghavi S."/>
                <person name="Benotmane M."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="Lapidus A."/>
                <person name="Mergeay M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference key="3">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text>Member of the two-component regulatory system CzcS/CzcR involved in the control of cobalt, zinc and cadmium homeostasis. Probably activates CzcR by phosphorylation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction>
            <text>ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.</text>
            <dbReference type="EC" id="2.7.13.3"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cell inner membrane</location>
            <topology evidence="4">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="induction">
        <text>By cadmium, lead, and zinc.</text>
    </comment>
    <dbReference type="EC" id="2.7.13.3"/>
    <dbReference type="EMBL" id="X71400">
        <property type="protein sequence ID" value="CAI11247.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="X98451">
        <property type="protein sequence ID" value="CAA67087.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP000354">
        <property type="protein sequence ID" value="ABF12836.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011229349.1">
        <property type="nucleotide sequence ID" value="NC_007971.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_145598.1">
        <property type="nucleotide sequence ID" value="NC_006466.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q44007"/>
    <dbReference type="SMR" id="Q44007"/>
    <dbReference type="EnsemblBacteria" id="ABF12836">
        <property type="protein sequence ID" value="ABF12836"/>
        <property type="gene ID" value="Rmet_5977"/>
    </dbReference>
    <dbReference type="GeneID" id="60820441"/>
    <dbReference type="KEGG" id="rme:Rmet_5977"/>
    <dbReference type="HOGENOM" id="CLU_000445_89_6_4"/>
    <dbReference type="OMA" id="HDEVQDV"/>
    <dbReference type="BRENDA" id="2.7.13.3">
        <property type="organism ID" value="231"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid pMOL30"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00082">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006290">
        <property type="entry name" value="CztS_silS_copS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003660">
        <property type="entry name" value="HAMP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003661">
        <property type="entry name" value="HisK_dim/P"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036097">
        <property type="entry name" value="HisK_dim/P_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004358">
        <property type="entry name" value="Sig_transdc_His_kin-like_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00672">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00512">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00344">
        <property type="entry name" value="BCTRLSENSOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00304">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00388">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47384">
        <property type="entry name" value="SSF47384"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="SSF55874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01386">
        <property type="entry name" value="cztS_silS_copS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50885">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0104">Cadmium</keyword>
    <keyword id="KW-0997">Cell inner membrane</keyword>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0170">Cobalt</keyword>
    <keyword id="KW-0418">Kinase</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0597">Phosphoprotein</keyword>
    <keyword id="KW-0614">Plasmid</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0732">Signal</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <keyword id="KW-0902">Two-component regulatory system</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000074746" description="Sensor protein CzcS">
        <location>
            <begin position="36"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="topological domain" description="Periplasmic" evidence="1">
        <location>
            <begin position="37"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="159"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="topological domain" description="Cytoplasmic" evidence="1">
        <location>
            <begin position="180"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="domain" description="HAMP" evidence="2">
        <location>
            <begin position="181"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="3">
        <location>
            <begin position="242"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="modified residue" description="Phosphohistidine; by autocatalysis" evidence="3">
        <location>
            <position position="245"/>
        </location>
    </feature>
    <feature type="sequence conflict" description="In Ref. 1; CAA67087." evidence="4" ref="1">
        <original>EAA</original>
        <variation>DR</variation>
        <location>
            <begin position="377"/>
            <end position="379"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="4"/>
    <sequence length="476" mass="52010" checksum="657329F4162C6326" modified="2006-07-11" version="2" precursor="true">MRPGTSITPLSLTRRLGLFFALVLSIALASMGAFAYYSLAAQLEARDDEVVKGKLEQVEHFLREVDGVQGVPAAQHRFDDLVRGYSDLIVRVTALDGRLLFRTGNDALLEGTDQAAVTGKSSLMFQSADAVLGRDGTRATVFVAKSGEDRKQVTARFRTTLVLGTTVGVILTALVGAAITRRELEPAHVLIKQINRISVERLSYRVDMPPKPTEVRDIASAFNAMLQRLEDGYQKLSRFSADLAHDLRTPLNNLIGHAEVALSRDRTGPEYVALVEESLVEYQRLARMIDAMLFLARADSANVALELTELQLNAELRKLSAYFSVLAEERSVVIRVSGDATLVADAILFQRAINNVLSNAVRHAWPNSMIDLVVRREAAHCCIDITNVGDPIPERELSLIFDRFFRGDRARSNSSQSTGLGLAIVLSIMELHGGDASAVSGLDGKTRFTLRFPLNGAEASARVSVGRPSQDRPVVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-04-26" modified="2022-08-03" version="141" xmlns="http://uniprot.org/uniprot">
    <accession>Q58AJ6</accession>
    <name>Q58AJ6_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">P-type ATPase involved in Pb(II) resistance PbrA</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">pbrA</name>
        <name evidence="8" type="ordered locus">Rmet_5947</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="8 9">
        <name>pMOL30</name>
    </geneLocation>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="9">Plasmid pMOL30</plasmid>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cell membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IB subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP000354">
        <property type="protein sequence ID" value="ABF12806.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011229367.1">
        <property type="nucleotide sequence ID" value="NC_007971.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_145622.1">
        <property type="nucleotide sequence ID" value="NC_006466.1"/>
    </dbReference>
    <dbReference type="SMR" id="Q58AJ6"/>
    <dbReference type="TCDB" id="3.A.3.6.4">
        <property type="family name" value="the p-type atpase (p-atpase) superfamily"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF12806">
        <property type="protein sequence ID" value="ABF12806"/>
        <property type="gene ID" value="Rmet_5947"/>
    </dbReference>
    <dbReference type="GeneID" id="60823111"/>
    <dbReference type="KEGG" id="rme:Rmet_5947"/>
    <dbReference type="HOGENOM" id="CLU_001771_6_4_4"/>
    <dbReference type="OMA" id="FAQPWET"/>
    <dbReference type="OrthoDB" id="237367at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid pMOL30"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019829">
        <property type="term" value="F:ATPase-coupled cation transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00371">
        <property type="entry name" value="HMA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1110.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023299">
        <property type="entry name" value="ATPase_P-typ_cyto_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018303">
        <property type="entry name" value="ATPase_P-typ_P_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023298">
        <property type="entry name" value="ATPase_P-typ_TM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008250">
        <property type="entry name" value="ATPase_P-typ_transduc_dom_A_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006121">
        <property type="entry name" value="HMA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036163">
        <property type="entry name" value="HMA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027256">
        <property type="entry name" value="P-typ_ATPase_IB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001757">
        <property type="entry name" value="P_typ_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044492">
        <property type="entry name" value="P_typ_ATPase_HD_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00403">
        <property type="entry name" value="HMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00941">
        <property type="entry name" value="CDATPASE"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00027">
        <property type="entry name" value="p-type_atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55008">
        <property type="entry name" value="SSF55008"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="SSF56784"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81653">
        <property type="entry name" value="SSF81653"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81665">
        <property type="entry name" value="SSF81665"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01525">
        <property type="entry name" value="ATPase-IB_hvy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01494">
        <property type="entry name" value="ATPase_P-type"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00154">
        <property type="entry name" value="ATPASE_E1_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50846">
        <property type="entry name" value="HMA_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0614">Plasmid</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-1278">Translocase</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="186"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="211"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="414"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="446"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="749"/>
            <end position="769"/>
        </location>
    </feature>
    <feature type="domain" description="HMA" evidence="7">
        <location>
            <begin position="30"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="domain" description="HMA" evidence="7">
        <location>
            <begin position="103"/>
            <end position="167"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU362081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ABF12806.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="799" mass="84174" checksum="EB68D302B5EB948F" modified="2005-04-26" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2005-01-04" modified="2021-09-29" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>Q5NV30</accession>
    <name>Q5NV30_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Plasmid segregation (Stability) protein ParM (Plasmid backbone),actin-like ATPase domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">parM</name>
        <name evidence="3" type="ordered locus">Rmet_6275</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3 4">
        <name>pMOL28</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid pMOL28</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000355">
        <property type="protein sequence ID" value="ABF13134.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011239920.1">
        <property type="nucleotide sequence ID" value="NC_007972.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_161660.1">
        <property type="nucleotide sequence ID" value="NC_006525.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF13134">
        <property type="protein sequence ID" value="ABF13134"/>
        <property type="gene ID" value="Rmet_6275"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6275"/>
    <dbReference type="HOGENOM" id="CLU_066433_0_0_4"/>
    <dbReference type="OMA" id="DVGYFTT"/>
    <dbReference type="OrthoDB" id="894872at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid pMOL28"/>
    </dbReference>
    <dbReference type="CDD" id="cd10227">
        <property type="entry name" value="ParM_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040607">
        <property type="entry name" value="ALP_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042051">
        <property type="entry name" value="ParM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009440">
        <property type="entry name" value="Plasmid_segregation_ParM/StbA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022389">
        <property type="entry name" value="PRTRC_protein-D"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17989">
        <property type="entry name" value="ALP_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06406">
        <property type="entry name" value="StbA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="SSF53067"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03739">
        <property type="entry name" value="PRTRC_D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ALP_N" evidence="2">
        <location>
            <begin position="14"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="domain" description="StbA" evidence="1">
        <location>
            <begin position="175"/>
            <end position="329"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF06406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF17989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF13134.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="341" mass="36609" checksum="A1EC94855DB6098D" modified="2005-01-04" version="1">MKQQNELIKHPTIAIDVGYGNTKTAWAMGAEIATNMFPSLAPLAANSSLNAFGGGVFKGRNVLNVEVDGARYEVGPGVSISGAHVHTGRSLSEDFATTAGYAALLAGALHYAGAREVDRLVLGLPVHNTQKYAAHLKERFAGTHNFGWGDIHVGSVLPLPQPLGTLIHYIQQRGKQYDPDNSYLVIDVGYFTTDWVVARGYTVDDTRSGGVPGGAARIYQQVASLITADRNQPDVGIERIDQAIRDAKPLVYFGEDLDMGPYLSEAMALTNQPVKEIQTRVGRTDDLRAIILTGGGAQLYAPAIRAAFPLNPIHMMDSPCFANVRGFYTIGAATRQASRAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-03-23" modified="2020-10-07" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>D3DXP1</accession>
    <name>D3DXP1_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_6447</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ADC45061.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADC45061">
        <property type="protein sequence ID" value="ADC45061"/>
        <property type="gene ID" value="Rmet_6447"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6447"/>
    <dbReference type="eggNOG" id="ENOG5032AGD">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_202368_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADC45061.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="54" mass="6199" checksum="FE3A196330753205" modified="2010-03-23" version="1">MQAWTNAWFEAAVELGFIRWPYVPDKEVVKRLHGYFNAGLMPMEAAQACFAAKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-03-23" modified="2020-10-07" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>D3DXQ3</accession>
    <name>D3DXQ3_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_6458</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ADC45073.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADC45073">
        <property type="protein sequence ID" value="ADC45073"/>
        <property type="gene ID" value="Rmet_6458"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6458"/>
    <dbReference type="HOGENOM" id="CLU_2938421_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADC45073.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="60" mass="6521" checksum="48E53B4C8BE213E2" modified="2010-03-23" version="1">MAAATKAIDIRLPLMRDWTLAVMRFIGRFLFLGQARATGNAPPRGGAFAQAVQQRGKTGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-03-23" modified="2020-10-07" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>D3DY12</accession>
    <name>D3DY12_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_6581</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ADC45182.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_6581"/>
    <dbReference type="EnsemblBacteria" id="ADC45182">
        <property type="protein sequence ID" value="ADC45182"/>
        <property type="gene ID" value="Rmet_6581"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6581"/>
    <dbReference type="HOGENOM" id="CLU_3423059_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADC45182.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="23" mass="2505" checksum="4AF15FF619CC37E5" modified="2010-03-23" version="1">MPDKSPKRFNRLASGAVIFTVAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-03-23" modified="2020-10-07" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>D3DY29</accession>
    <name>D3DY29_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_6598</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ADC45199.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_6598"/>
    <dbReference type="EnsemblBacteria" id="ADC45199">
        <property type="protein sequence ID" value="ADC45199"/>
        <property type="gene ID" value="Rmet_6598"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6598"/>
    <dbReference type="HOGENOM" id="CLU_2737162_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADC45199.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="71" mass="8074" checksum="F739E36F21D5415E" modified="2010-03-23" version="1">MRGTRRDFSTKLSTGVTDTLEKPPRIIDLGSHFRFYFKLMLRRTQTRSENPVTPAMHNHRAAGLHHAGAGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-03-23" modified="2020-10-07" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>D3DYB6</accession>
    <name>D3DYB6_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_6704</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ADC45286.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_6704"/>
    <dbReference type="EnsemblBacteria" id="ADC45286">
        <property type="protein sequence ID" value="ADC45286"/>
        <property type="gene ID" value="Rmet_6704"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6704"/>
    <dbReference type="HOGENOM" id="CLU_2754983_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADC45286.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="70" mass="8009" checksum="74F090AE8C363904" modified="2010-03-23" version="1">MGHICLLYAACWVAEPARTREKLMAGAQYIKLRSREQPPKWGWVGGGMEGVSQVANQALWPLALVWWRER</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="52" xmlns="http://uniprot.org/uniprot">
    <accession>Q1L9L3</accession>
    <name>Q1L9L3_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Conjugal transfer protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">trbD</name>
        <name evidence="2" type="ordered locus">Rmet_6304</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2 3">
        <name>pMOL28</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid pMOL28</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000355">
        <property type="protein sequence ID" value="ABF13163.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008649455.1">
        <property type="nucleotide sequence ID" value="NC_007972.2"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF13163">
        <property type="protein sequence ID" value="ABF13163"/>
        <property type="gene ID" value="Rmet_6304"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6304"/>
    <dbReference type="HOGENOM" id="CLU_2258417_0_0_4"/>
    <dbReference type="OMA" id="FRRPYGF"/>
    <dbReference type="OrthoDB" id="1672986at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid pMOL28"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="45"/>
            <end position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF13163.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="109" mass="12402" checksum="78986737AB735661" modified="2006-05-30" version="1">MRKHHVSIGLSMPRLIAGAVRSLAIANGTIVALLIYVTYRSGWQIPLAVFVYGLCSHALLAWLTSRDPWWNQILNVYNQYGDLYESLPWHGKSHAVFRRPYGFDSDLPC</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-10-07" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LA76</accession>
    <name>Q1LA76_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_6091</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="1 2">
        <name>pMOL30</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid pMOL30</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000354">
        <property type="protein sequence ID" value="ABF12950.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF12950">
        <property type="protein sequence ID" value="ABF12950"/>
        <property type="gene ID" value="Rmet_6091"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6091"/>
    <dbReference type="HOGENOM" id="CLU_2275144_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid pMOL30"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF12950.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="102" mass="11149" checksum="795758E771FC7BF6" modified="2006-05-30" version="1">MVIRERSSRVKRHSAHIPCKVASVDALASSMGLLMTWLISYQSATALPAQGYTPRFRLRCMDTTTPEHPVTRILKKGLENARHHGNITGSVAPGQIASALAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="64" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LAR8</accession>
    <name>Q1LAR8_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_5899</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12758.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518106.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5899"/>
    <dbReference type="EnsemblBacteria" id="ABF12758">
        <property type="protein sequence ID" value="ABF12758"/>
        <property type="gene ID" value="Rmet_5899"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5899"/>
    <dbReference type="eggNOG" id="COG0247">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023081_1_0_4"/>
    <dbReference type="OMA" id="DKVASAC"/>
    <dbReference type="OrthoDB" id="647535at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004017">
        <property type="entry name" value="Cys_rich_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02754">
        <property type="entry name" value="CCG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CCG" evidence="1">
        <location>
            <begin position="16"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="domain" description="CCG" evidence="1">
        <location>
            <begin position="149"/>
            <end position="233"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02754"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF12758.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="261" mass="28162" checksum="F3D6FDAA7E135149" modified="2006-05-30" version="1">MESRQYPTTAPQQVYLFGTCLVDLFVPQAGLDAVRLLEREGLTVHFPRGQSCCGQPAYSSGNPEQARKVARAQLDLFREPWPIIVPSGSCAGMMRHHWPTLFADDPSDPDAAALARDIASRVYELAEFLLNVLHVRFDATPADAPHERVVLHTSCAARREMGTRAHGVALLDALPGVTRVEHEHESECCGFGGTFSLKHADISGAMVRDKVASACATGCDRLVSADCGCLLNIGHAAAHTGAPLPVEHLASFLWRRTGGAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="100" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LAS0</accession>
    <name>Q1LAS0_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Iron-sulfur cluster binding protein putative oxidoreductase subunit with NAD(P)-binding domain and ferridoxin-like domain</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">Rmet_5897</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="10">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="10">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12756.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518104.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5897"/>
    <dbReference type="EnsemblBacteria" id="ABF12756">
        <property type="protein sequence ID" value="ABF12756"/>
        <property type="gene ID" value="Rmet_5897"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5897"/>
    <dbReference type="eggNOG" id="COG1139">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027059_2_0_4"/>
    <dbReference type="OMA" id="HCPVYDK"/>
    <dbReference type="OrthoDB" id="688908at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019516">
        <property type="term" value="P:lactate oxidation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1060.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10420">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004452">
        <property type="entry name" value="4Fe-4S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024569">
        <property type="entry name" value="4Fe-4S-bd_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024185">
        <property type="entry name" value="FTHF_cligase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009051">
        <property type="entry name" value="Helical_ferredxn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003741">
        <property type="entry name" value="LUD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037171">
        <property type="entry name" value="NagB/RpiA_transferase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47153">
        <property type="entry name" value="PTHR47153"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11870">
        <property type="entry name" value="DUF3390"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13183">
        <property type="entry name" value="Fer4_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02589">
        <property type="entry name" value="LUD_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100950">
        <property type="entry name" value="SSF100950"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00273">
        <property type="entry name" value="TIGR00273"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0249">Electron transport</keyword>
    <keyword evidence="5" id="KW-0408">Iron</keyword>
    <keyword evidence="6" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0614">Plasmid</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="8">
        <location>
            <begin position="308"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="453"/>
            <end position="481"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ABF12756.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="481" mass="53110" checksum="98CF02C9C8DFD599" modified="2006-05-30" version="1">MSQQPLHFVPTEDFRARARAALDDPKLRKSFRGAMDFLQQKRAVQFPDGDELEHLRDLGEAIRQHALANLPDLLVELEGNLTAAGVQVHWAETADEANAIILRIAQGHAARRVIKGKSMASEEMELNHYLAERGIDCIESDMGEYIVQLAGEKPSHIVMPAIHKTKADIATLFEQHIPDTPYTEDVDALIQTGRRALRQAFVEADIGLSGVNFAAADTGTLWLVENEGNGRLSTTVPDVHIAVMGMEKVVAKLSHIVPLSSLLTRSATGQPITTYFNLISSPRKPHEQDGPREVHLVLLDNGRSQAYADEQLRATLQCIRCGACMNHCPVYTRIGGHAYGTTYPGPIGKIISPHLLGLDATADLATASSLCGACGEVCPVRIPIPQLLVRLRTEANRNPDEAVPHPLKGQGAKFSRREHLVWRFWSGAFAHPTTYRLFRWAATRLRALTPSRQMGWTQHRKPLTPAPKSLSDLLDARNQPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LAY8</accession>
    <name>Q1LAY8_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 6">Phosphate-binding protein PstS</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">pstS1</name>
        <name evidence="9" type="ordered locus">Rmet_5829</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="10">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="10">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 6">Part of the ABC transporter complex PstSACB involved in phosphate import.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3 6">The complex is composed of two ATP-binding proteins (PstB), two transmembrane proteins (PstC and PstA) and a solute-binding protein (PstS).</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the PstS family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12688.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5829"/>
    <dbReference type="EnsemblBacteria" id="ABF12688">
        <property type="protein sequence ID" value="ABF12688"/>
        <property type="gene ID" value="Rmet_5829"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5829"/>
    <dbReference type="eggNOG" id="COG0226">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_034528_1_0_4"/>
    <dbReference type="OMA" id="NCENCEF"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043190">
        <property type="term" value="C:ATP-binding cassette (ABC) transporter complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042301">
        <property type="term" value="F:phosphate ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035435">
        <property type="term" value="P:phosphate ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005673">
        <property type="entry name" value="ABC_phos-bd_PstS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024370">
        <property type="entry name" value="PBP_domain"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12849">
        <property type="entry name" value="PBP_like_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002756">
        <property type="entry name" value="PstS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00975">
        <property type="entry name" value="3a0107s03"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0592">Phosphate transport</keyword>
    <keyword evidence="9" id="KW-0614">Plasmid</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193255" description="Phosphate-binding protein PstS" evidence="7">
        <location>
            <begin position="20"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="domain" description="PBP_domain" evidence="8">
        <location>
            <begin position="20"/>
            <end position="302"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002841"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00021889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR002756"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF12849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ABF12688.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="333" mass="35766" checksum="D2AE998DBBC582D0" modified="2006-05-30" version="1">MGRHIAALALLFAAVSAFAGDVTGAGSTFVYPLMAKWAATYYARTGRQIDYQPIGSGNGIRQIKAASITFGTTDMPLKPEELERAGLAQFPIAVGGVVPVINLQGIGAGQIHMTGALLADIYRGKIANWNDAAIASANPGVQFPDLKITVVHRSDGSGTTFNWTDYLSKVSPEWKAAVGAGTTVRWPIGMNASGNEGVSLYIHNIRGAIGYVELTYALQKSLPYALVQNRDGLYVKPSRESFSAAVTAVEWNPRLDFYQVLTNAPGPNAWPITGTVFVLMQRSAAHPSNTKDALAFFQWALAEGQSDASAEHYVALPPELVKQVIAYWAETFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="107" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LAZ5</accession>
    <name>Q1LAZ5_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Transcriptional regulator, LuxR family, ATP-dependent</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Rmet_5822</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="5">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="5">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12681.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518032.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF12681">
        <property type="protein sequence ID" value="ABF12681"/>
        <property type="gene ID" value="Rmet_5822"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5822"/>
    <dbReference type="eggNOG" id="COG2909">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006325_2_0_4"/>
    <dbReference type="OMA" id="MIEGRMD"/>
    <dbReference type="OrthoDB" id="1377603at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06170">
        <property type="entry name" value="LuxR_C_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016032">
        <property type="entry name" value="Sig_transdc_resp-reg_C-effctor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041617">
        <property type="entry name" value="TPR_MalT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000792">
        <property type="entry name" value="Tscrpt_reg_LuxR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00196">
        <property type="entry name" value="GerE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17874">
        <property type="entry name" value="TPR_MalT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00038">
        <property type="entry name" value="HTHLUXR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00421">
        <property type="entry name" value="HTH_LUXR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46894">
        <property type="entry name" value="SSF46894"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="SSF48452"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50043">
        <property type="entry name" value="HTH_LUXR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0614">Plasmid</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH luxR-type" evidence="3">
        <location>
            <begin position="846"/>
            <end position="911"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF12681.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="914" mass="101000" checksum="4A36A8793B6DEAE7" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LB94</accession>
    <name>Q1LB94_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_5723</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12582.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011520123.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5723"/>
    <dbReference type="EnsemblBacteria" id="ABF12582">
        <property type="protein sequence ID" value="ABF12582"/>
        <property type="gene ID" value="Rmet_5723"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5723"/>
    <dbReference type="HOGENOM" id="CLU_2036076_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF12582.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="121" mass="13624" checksum="1FA84B5D97C89DC4" modified="2006-05-30" version="1">MTSRIEALEHRIAALESEIDRLNQTQKTGPNSDDLELVIGFVQGLSWMLETHVRVQAEYDQNVINNFAAVLLYESERLHPHVPHRDSIAMTLRLMAHSLTDSRAESGAFLRGMPAPGQSHH</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LBK4</accession>
    <name>Q1LBK4_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Permease of the major facilitator superfamily</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Rmet_5613</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="8">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="8">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12472.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5613"/>
    <dbReference type="EnsemblBacteria" id="ABF12472">
        <property type="protein sequence ID" value="ABF12472"/>
        <property type="gene ID" value="Rmet_5613"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5613"/>
    <dbReference type="eggNOG" id="COG2814">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001265_62_0_4"/>
    <dbReference type="OMA" id="ACAPVFM"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="SSF103473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0614">Plasmid</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="38"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="71"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="105"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="128"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="167"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="191"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="234"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="276"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="305"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="330"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="372"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="402"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="domain" description="MFS" evidence="6">
        <location>
            <begin position="37"/>
            <end position="427"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABF12472.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="432" mass="45531" checksum="D07E50298352D8EC" modified="2006-05-30" version="1">MCPRRIAREPLQQETKVNTTFQQESTAAAAETNTSRGIALLTFAFILSQFYRSCLAVIAPELQHDFGLSPAGFGALSSCFFLAFAVAQIPVGIAFDRYGVGRPTAVLLGVGAVSAIGFVVAPNAGMAMLVQMGLGLACAPVFMGLLHYASEQLSERQYMRVGSRSNALGMIGALCATAPLGWASQHVGWRPAMALAAVCMVVACYGVWRFVRDDGHEQARQAPMSAMLVESGKLLGYWPLWTLIPLCVAMSAGTGFRNAWSGPYLADVFGLQAGTRGMALMLLSLAGFLTAFALPVLVRRSSLKAAIGIWGCLATAGGIALTIWPDAGVASGVGMMALLSTIGMLHPLVMAQGRALIPAPARGRGLGLLNTFVFLGSALTSWGFGLIANVGHLRDWPVAHTYSAIFLSAVVVVALSLVPYYFSPISAPAPRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="59" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LBM1</accession>
    <name>Q1LBM1_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Putative outer membrane lytic transglycosylase MltA-interacting MipA</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Rmet_5596</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="7">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="7">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the MipA/OmpV family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12455.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008642631.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5596"/>
    <dbReference type="EnsemblBacteria" id="ABF12455">
        <property type="protein sequence ID" value="ABF12455"/>
        <property type="gene ID" value="Rmet_5596"/>
    </dbReference>
    <dbReference type="GeneID" id="60824411"/>
    <dbReference type="KEGG" id="rme:Rmet_5596"/>
    <dbReference type="eggNOG" id="COG3713">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_062990_1_1_4"/>
    <dbReference type="OMA" id="LGEWQYS"/>
    <dbReference type="OrthoDB" id="1497770at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009279">
        <property type="term" value="C:cell outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010583">
        <property type="entry name" value="MipA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38776">
        <property type="entry name" value="PTHR38776"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06629">
        <property type="entry name" value="MipA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0998">Cell outer membrane</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0614">Plasmid</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193040" evidence="5">
        <location>
            <begin position="43"/>
            <end position="299"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABF12455.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="299" mass="32550" checksum="D21C701FD545EC17" modified="2006-05-30" version="1">MSTNSDYQAGVAHGRYPRALILWLATAMFALVALLAAPRAHAQTPAPLAEWQYSVGVPLEKLFEGPPPDWTVRLGMAARISPYYDGTGNYYPLAGPAIDIRYRDQGFLSTGEGLGWNIVHSDHWRAGIALTYDFGRREKHDHDHLAGLGNIGMAVESKLFAEYVVSKEFPLVIRANARRQFGGANGWLGDLGAYMPLPGSSDKFAWFAGPTMTVADSAYMSRWYGVTAEQSARSGLPQYHAGAGIRSYGGGVSAMWFFGKHWFATADFGIAALVGDARNSPVVSHSANMTGDISIMYEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="67" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LBN9</accession>
    <name>Q1LBN9_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">PepSY domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_5578</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12437.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519987.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5578"/>
    <dbReference type="EnsemblBacteria" id="ABF12437">
        <property type="protein sequence ID" value="ABF12437"/>
        <property type="gene ID" value="Rmet_5578"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5578"/>
    <dbReference type="eggNOG" id="COG5591">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_147864_1_0_4"/>
    <dbReference type="OMA" id="CYEIYGT"/>
    <dbReference type="OrthoDB" id="2084476at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025711">
        <property type="entry name" value="PepSY"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13670">
        <property type="entry name" value="PepSY_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004192988" description="PepSY domain-containing protein" evidence="1">
        <location>
            <begin position="20"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="domain" description="PepSY" evidence="2">
        <location>
            <begin position="4"/>
            <end position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF12437.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="88" mass="9687" checksum="9C23D993C8DA815B" modified="2006-05-30" version="1">MQKIAAFLVAGVFSTAAFAGANCSKHPKSEWMPEADAKAKIQEQGYNIKKFKVDGNCYEIYGTTKDGKKAEIYFDTKTLEIVKSEIGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="100" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LBZ7</accession>
    <name>Q1LBZ7_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Transcriptional regulator, GntR family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Rmet_5470</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="5">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="5">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12329.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519875.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5470"/>
    <dbReference type="EnsemblBacteria" id="ABF12329">
        <property type="protein sequence ID" value="ABF12329"/>
        <property type="gene ID" value="Rmet_5470"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5470"/>
    <dbReference type="eggNOG" id="COG2188">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_063236_3_2_4"/>
    <dbReference type="OMA" id="ATTLECK"/>
    <dbReference type="OrthoDB" id="1775418at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006744">
        <property type="term" value="P:ubiquinone biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd07377">
        <property type="entry name" value="WHTH_GntR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1410.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028978">
        <property type="entry name" value="Chorismate_lyase_/UTRA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000524">
        <property type="entry name" value="Tscrpt_reg_HTH_GntR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011663">
        <property type="entry name" value="UTRA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00392">
        <property type="entry name" value="GntR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07702">
        <property type="entry name" value="UTRA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00035">
        <property type="entry name" value="HTHGNTR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00345">
        <property type="entry name" value="HTH_GNTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00866">
        <property type="entry name" value="UTRA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64288">
        <property type="entry name" value="SSF64288"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50949">
        <property type="entry name" value="HTH_GNTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0456">Lyase</keyword>
    <keyword evidence="4" id="KW-0614">Plasmid</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0831">Ubiquinone biosynthesis</keyword>
    <feature type="domain" description="HTH gntR-type" evidence="3">
        <location>
            <begin position="1"/>
            <end position="68"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF12329.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="242" mass="26506" checksum="69A260A0CC16072F" modified="2006-05-30" version="1">MRYTELANTLVKGITEGKFAVGDQLPSELALADQYDVSRTTVRAALDIVEGLGLITRRRRAGTIVCSNTTSNTYTKSVNTIEDLVNYASHTEREVLGVANVVADERLAAALECKPGATWLRIRMLRTEMDSEGTPLCWNDAYVDPDIGQRIAPLVPNGTGLLSQIIEQTVGVTVADIKQTIGATTITGEMARRLLVEPGVAGLEITRTYLDDAKKPFLITVNTYPADKFRFTFWMHRAPTSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LC44</accession>
    <name>Q1LC44_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Arylsulfatase (AS) (Aryl-sulfate sulphohydrolase)</fullName>
            <ecNumber evidence="6">3.1.6.1</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">atsA</name>
        <name evidence="6" type="ordered locus">Rmet_5423</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="7">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="7">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="PTM">
        <text evidence="3">The conversion to 3-oxoalanine (also known as C-formylglycine, FGly), of a serine or cysteine residue in prokaryotes and of a cysteine residue in eukaryotes, is critical for catalytic activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the sulfatase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.6.1" evidence="6"/>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12282.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519830.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5423"/>
    <dbReference type="EnsemblBacteria" id="ABF12282">
        <property type="protein sequence ID" value="ABF12282"/>
        <property type="gene ID" value="Rmet_5423"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5423"/>
    <dbReference type="eggNOG" id="COG3119">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006332_11_1_4"/>
    <dbReference type="OMA" id="NTVHAAT"/>
    <dbReference type="OrthoDB" id="1067869at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004065">
        <property type="term" value="F:arylsulfatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.720.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017850">
        <property type="entry name" value="Alkaline_phosphatase_core_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024607">
        <property type="entry name" value="Sulfatase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000917">
        <property type="entry name" value="Sulfatase_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00884">
        <property type="entry name" value="Sulfatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53649">
        <property type="entry name" value="SSF53649"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00149">
        <property type="entry name" value="SULFATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0614">Plasmid</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193312" evidence="4">
        <location>
            <begin position="29"/>
            <end position="660"/>
        </location>
    </feature>
    <feature type="domain" description="Sulfatase" evidence="5">
        <location>
            <begin position="60"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="modified residue" description="3-oxoalanine (Cys)" evidence="3">
        <location>
            <position position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008779"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR600917-51"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABF12282.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="660" mass="70890" checksum="2125DA29B7713941" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LCK1</accession>
    <name>Q1LCK1_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Flagellar hook-length control protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">fliK</name>
        <name evidence="6" type="ordered locus">Rmet_5266</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="7">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="7">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Controls the length of the flagellar hook.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the FliK family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12125.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519671.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5266"/>
    <dbReference type="EnsemblBacteria" id="ABF12125">
        <property type="protein sequence ID" value="ABF12125"/>
        <property type="gene ID" value="Rmet_5266"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5266"/>
    <dbReference type="eggNOG" id="COG3144">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_575849_0_0_4"/>
    <dbReference type="OMA" id="TQVTFMS"/>
    <dbReference type="OrthoDB" id="1930992at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009424">
        <property type="term" value="C:bacterial-type flagellum hook"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044780">
        <property type="term" value="P:bacterial-type flagellum assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.750.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001635">
        <property type="entry name" value="Flag_hook_Flik"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021136">
        <property type="entry name" value="Flagellar_hook_control-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038610">
        <property type="entry name" value="FliK-like_C_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02120">
        <property type="entry name" value="Flg_hook"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01007">
        <property type="entry name" value="FLGHOOKFLIK"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1005">Bacterial flagellum biogenesis</keyword>
    <keyword evidence="6" id="KW-0966">Cell projection</keyword>
    <keyword evidence="6" id="KW-0969">Cilium</keyword>
    <keyword evidence="6" id="KW-0282">Flagellum</keyword>
    <keyword evidence="6" id="KW-0614">Plasmid</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Flg_hook" evidence="5">
        <location>
            <begin position="362"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="23"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="249"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="438"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="42"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="438"/>
            <end position="464"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003944"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022795"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF02120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABF12125.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="495" mass="48539" checksum="C9C6C0B5D6DBE7F9" modified="2006-05-30" version="1">MIDISQLIGNTANAALKTLTQSNAASTAAADAKAFQDALSRARDSAPQSAQPVASKPRDSSQTSAPKEDASQNTRQQTTQASTSGASSTPQQKDNGKTNATTGKKDDSTQASDDGSTPSSDAQAAAAALAAAMLAQQAAPATTALTGGAAADAAANGAGLAGANGLPLAAQTAATTTATQANPATDAATLLAQAADAQSATTTAGTAAKGTTPSPADTLATIAQQRAAAQNSTPTAADKIAAALNNPAQTGTQTGAQDARGQNAGQQQTGLGHGAQPTLQADTTQASQTPIATPYAMAQASARTGEGTDTGATNPSSAAMLASALSAQSQTNAAQATAAAAAARPAVAPNLGDSQWAQALGQQMVRLSTQGSQTAELDLNPPNLGPLKVVLNVVNDQAQAQFVSPHQAVRAAVEAALPHLRTSMAEAGIQLGQTSVGSDGFAQAGNGGQQQQRQPGSGSAFAQAAGLTQDAPVQATAAAPRAPRVLARGEVDTFA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-10-07" version="67" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LCQ2</accession>
    <name>Q1LCQ2_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_5215</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12074.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5215"/>
    <dbReference type="EnsemblBacteria" id="ABF12074">
        <property type="protein sequence ID" value="ABF12074"/>
        <property type="gene ID" value="Rmet_5215"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5215"/>
    <dbReference type="eggNOG" id="COG1075">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_719009_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="SSF53474"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="21"/>
            <end position="43"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF12074.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="396" mass="42551" checksum="6C87EF35E47EE230" modified="2006-05-30" version="1">MQLDTLSTESQADGATPAFRVIRAWLASFACSTALVASLVLGACASKPIVPYSTDTPPLVLVPAPLAGVTDKRGRFREIYCAVLQTRGSALPDYRPCDEALTRVGSEPAGTGKPVDLGPSKRHLIAAVVAGIGYECFKPWLDPPGTVVTHLREFGYDATMITVDALSSSTNNARQIRDAIMDMSLAVNEPRIVLMGYSKGAPDILEAVVTYPEIRSRVAAVVSAAGAVGGSPLANDAEQYQADLLRHFPGATCSSGDGGAVESLRPQTRKAWLAQNPLPYDLPIYSIVTFPQPERISSILRSSYNKLSRIDARNDSQMIFYDQIVPGSTLLGYVNADHWALAVPIARTHDTVGSLFVTQNAYPREALAEALLRFVEEDMAVPASHVPSSVRRRPAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-10-07" version="51" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LCU2</accession>
    <name>Q1LCU2_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_5175</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12034.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF12034">
        <property type="protein sequence ID" value="ABF12034"/>
        <property type="gene ID" value="Rmet_5175"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5175"/>
    <dbReference type="eggNOG" id="ENOG50347DP">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_193003_0_0_4"/>
    <dbReference type="OMA" id="THGSEAK"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF12034.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="87" mass="9623" checksum="B401735ECC2DA447" modified="2006-05-30" version="1">MNQDFTPFTVSVYPIQQEPGIWFASYMISEYRGGAERVVANVSMRHVTHGSEAKAKQAARNAGDGVVAHMGRQSFERHPTNLPTQCA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LCV1</accession>
    <name>Q1LCV1_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Two-component sensor histidine kinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Rmet_5166</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="6">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="6">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12025.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519570.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5166"/>
    <dbReference type="EnsemblBacteria" id="ABF12025">
        <property type="protein sequence ID" value="ABF12025"/>
        <property type="gene ID" value="Rmet_5166"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5166"/>
    <dbReference type="eggNOG" id="COG4585">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014388_0_0_4"/>
    <dbReference type="OMA" id="VWYRIDW"/>
    <dbReference type="OrthoDB" id="1755994at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011623">
        <property type="entry name" value="7TMR_DISM_rcpt_extracell_dom1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008979">
        <property type="entry name" value="Galactose-bd-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011712">
        <property type="entry name" value="Sig_transdc_His_kin_sub3_dim/P"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07695">
        <property type="entry name" value="7TMR-DISM_7TM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07730">
        <property type="entry name" value="HisKA_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49785">
        <property type="entry name" value="SSF49785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="SSF55874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0614">Plasmid</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193070" evidence="3">
        <location>
            <begin position="23"/>
            <end position="640"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="184"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="212"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="243"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="276"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="301"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="332"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="361"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="domain" description="HATPase_c" evidence="4">
        <location>
            <begin position="526"/>
            <end position="621"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="621"/>
            <end position="640"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="625"/>
            <end position="640"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABF12025.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="640" mass="71696" checksum="2CA6A48382F101D3" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LCY2</accession>
    <name>Q1LCY2_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Transcriptional regulator, LysR family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_5135</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the LysR transcriptional regulatory family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11994.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519541.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5135"/>
    <dbReference type="EnsemblBacteria" id="ABF11994">
        <property type="protein sequence ID" value="ABF11994"/>
        <property type="gene ID" value="Rmet_5135"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5135"/>
    <dbReference type="eggNOG" id="COG0583">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039613_6_4_4"/>
    <dbReference type="OMA" id="LTEERWA"/>
    <dbReference type="OrthoDB" id="990666at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005119">
        <property type="entry name" value="LysR_subst-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000847">
        <property type="entry name" value="Tscrpt_reg_HTH_LysR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00126">
        <property type="entry name" value="HTH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03466">
        <property type="entry name" value="LysR_substrate"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00039">
        <property type="entry name" value="HTHLYSR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50931">
        <property type="entry name" value="HTH_LYSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH lysR-type" evidence="2">
        <location>
            <begin position="1"/>
            <end position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF11994.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="296" mass="32683" checksum="71F0451D2131593E" modified="2006-05-30" version="1">METRDLEYVLAVETHGGIGRAAEALDMTQPALTKAVQRVEAQLGVPLFERTTLGMRVTQAGAVFLERARRIRLEYEDAIKEMRGIQTGEQGMLRLGYSPSMPNALVLGACRQLIRERPVARLRLTMRVARELMDLLQAGELDLAIAPLPRQANPALVSRELFTDRLAVVADENHPLLRRRNLTLADLTDQQWLLPSSHVLIRQQIDAAFTELGLPAPILRVETDFGSTALFDLVRGTEMLCIAGSTSNGAQTGLRPIALRPDALDLGRRVGVMSRAGAYLSPLALRMIGIFEARMQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LD78</accession>
    <name>Q1LD78_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_5036</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11898.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519449.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF11898">
        <property type="protein sequence ID" value="ABF11898"/>
        <property type="gene ID" value="Rmet_5036"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5036"/>
    <dbReference type="HOGENOM" id="CLU_2635496_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF11898.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="77" mass="8236" checksum="CDE082EA6220A1BE" modified="2006-05-30" version="1">MSTSIVKVLVTQDNHWAVESDGQLNAYASRGAAIAAGVHKAIKERAMLMIYEREAHASEPIEPIESCDVGVLASVQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="60" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LD79</accession>
    <name>Q1LD79_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_5035</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11897.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519448.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF11897">
        <property type="protein sequence ID" value="ABF11897"/>
        <property type="gene ID" value="Rmet_5035"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5035"/>
    <dbReference type="HOGENOM" id="CLU_179056_4_0_4"/>
    <dbReference type="OMA" id="THNGWAV"/>
    <dbReference type="OrthoDB" id="2065458at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018691">
        <property type="entry name" value="DUF2188"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09954">
        <property type="entry name" value="DUF2188"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="52"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="52"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF11897.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="72" mass="7956" checksum="E22B4670E070A071" modified="2006-05-30" version="1">MSGDIHVLPHGSEWAIAIEGTGTRTRYQTREAAIDIVTQLAKRTRAELLIHGRDGQIHERSTFGHGSPHTRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LDK9</accession>
    <name>Q1LDK9_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative transcriptional regulator, LysR family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_4905</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the LysR transcriptional regulatory family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11767.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4905"/>
    <dbReference type="EnsemblBacteria" id="ABF11767">
        <property type="protein sequence ID" value="ABF11767"/>
        <property type="gene ID" value="Rmet_4905"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4905"/>
    <dbReference type="eggNOG" id="COG0583">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039613_6_5_4"/>
    <dbReference type="OMA" id="FQRELVM"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005119">
        <property type="entry name" value="LysR_subst-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000847">
        <property type="entry name" value="Tscrpt_reg_HTH_LysR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00126">
        <property type="entry name" value="HTH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03466">
        <property type="entry name" value="LysR_substrate"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00039">
        <property type="entry name" value="HTHLYSR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50931">
        <property type="entry name" value="HTH_LYSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH lysR-type" evidence="2">
        <location>
            <begin position="15"/>
            <end position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF11767.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="301" mass="33169" checksum="4B28B36DD44625FF" modified="2006-05-30" version="1">MTEHRKFRCLNPALLRYFKTFLVAAETGSFSSAGARLGLTQSAVSTQIRRLEEDLGCTLFDRSGKSVTLSEEGRQMLADASRIVALYESMKGARLQRDTAPLDLGAVSTVQASLLPRAMQRFRVAFPDTHVNIVPGMSTQLLTQVDARELDIAVLVKPRLGIPPELKWVPLVKERFVAVAPKGAPADLKTLLSTVPFIRYNRHSTGGQLVDRYLKRHRLWVREGMELDEPAVILQMVGEGLGCAIIPCELVPLAQTPNVEQVPMPGMPMYREIGVLVRQSALKRASAAALIDAFVAACHER</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="103" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LDN0</accession>
    <name>Q1LDN0_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Benzoate 1,2-dioxygenase electron transfer component</fullName>
            <ecNumber evidence="7">1.18.1.3</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">benC</name>
        <name evidence="7" type="ordered locus">Rmet_4884</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="8">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="8">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EC" id="1.18.1.3" evidence="7"/>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11746.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519305.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4884"/>
    <dbReference type="EnsemblBacteria" id="ABF11746">
        <property type="protein sequence ID" value="ABF11746"/>
        <property type="gene ID" value="Rmet_4884"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4884"/>
    <dbReference type="eggNOG" id="COG0543">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG0633">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003827_7_0_4"/>
    <dbReference type="OMA" id="GTCKCHC"/>
    <dbReference type="OrthoDB" id="1834577at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051213">
        <property type="term" value="F:dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008860">
        <property type="term" value="F:ferredoxin-NAD+ reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00207">
        <property type="entry name" value="fer2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036010">
        <property type="entry name" value="2Fe-2S_ferredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001041">
        <property type="entry name" value="2Fe-2S_ferredoxin-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006058">
        <property type="entry name" value="2Fe2S_fd_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012675">
        <property type="entry name" value="Beta-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008333">
        <property type="entry name" value="Cbr1-like_FAD-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017927">
        <property type="entry name" value="FAD-bd_FR_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001709">
        <property type="entry name" value="Flavoprot_Pyr_Nucl_cyt_Rdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039261">
        <property type="entry name" value="FNR_nucleotide-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001433">
        <property type="entry name" value="OxRdtase_FAD/NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017938">
        <property type="entry name" value="Riboflavin_synthase-like_b-brl"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00970">
        <property type="entry name" value="FAD_binding_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00111">
        <property type="entry name" value="Fer2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00175">
        <property type="entry name" value="NAD_binding_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00371">
        <property type="entry name" value="FPNCR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52343">
        <property type="entry name" value="SSF52343"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54292">
        <property type="entry name" value="SSF54292"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63380">
        <property type="entry name" value="SSF63380"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00197">
        <property type="entry name" value="2FE2S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51085">
        <property type="entry name" value="2FE2S_FER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51384">
        <property type="entry name" value="FAD_FR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0001">2Fe-2S</keyword>
    <keyword evidence="7" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="3" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-0614">Plasmid</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="2Fe-2S ferredoxin-type" evidence="5">
        <location>
            <begin position="3"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="domain" description="FAD-binding FR-type" evidence="6">
        <location>
            <begin position="105"/>
            <end position="205"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABF11746.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="339" mass="36117" checksum="778E5992C3F8A172" modified="2006-05-30" version="1">MEHTIALQFEDGVTRFITCGDNETLSDAAYRQQINIPLDCRDGACGTCRGLCESGHYDLPESSYIEDALTAEEAAKGYVLACKTRPRSDCVIRVPASSAACKTGVTRFEGAIAAVAPLSDSTIGFSIDLDDPAALSFLPGQYVNVEIPGSGLTRAYSFSSPPGASQAAFVVRNVPQGRMSGFLAGEAQTGQRMAFSGPFGSFYLRSVTRPVLFLAGGTGIAPFLSMLDVLAASGSTHPVRMVYGVTHDIDLVATERLEAAAASIDGFTYRTCIADAASTHERKGYVTTHVDPTWINHGDVDIYLCGPVAMVEAVRDWLRDAGITPANFYYEKFSASAEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="98" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LDN5</accession>
    <name>Q1LDN5_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Transcriptional regulator, LysR family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">catM</name>
        <name evidence="3" type="ordered locus">Rmet_4879</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the LysR transcriptional regulatory family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11741.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519300.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4879"/>
    <dbReference type="EnsemblBacteria" id="ABF11741">
        <property type="protein sequence ID" value="ABF11741"/>
        <property type="gene ID" value="Rmet_4879"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4879"/>
    <dbReference type="eggNOG" id="COG0583">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039613_6_4_4"/>
    <dbReference type="OMA" id="APPGINI"/>
    <dbReference type="OrthoDB" id="1439189at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005119">
        <property type="entry name" value="LysR_subst-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000847">
        <property type="entry name" value="Tscrpt_reg_HTH_LysR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00126">
        <property type="entry name" value="HTH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03466">
        <property type="entry name" value="LysR_substrate"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00039">
        <property type="entry name" value="HTHLYSR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50931">
        <property type="entry name" value="HTH_LYSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH lysR-type" evidence="2">
        <location>
            <begin position="1"/>
            <end position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF11741.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="291" mass="32357" checksum="D81A73692C8498CC" modified="2006-05-30" version="1">MELRHLRYFQTVAQELNVTRAAEKLHMAQPPLSRQIRQFEEEVGVALFDRVGRGLRLTEAGRFLLEQSLQLTQRLEETLESTRRIGRNEKRWFGIGFVPSVLYGVLPELIRELRGDDSQVEVGLTEMITVQQLEALKSGRIDLAFGRLTFNDPAIVQQVVMAESLVAAIPGTAPTPTAPFDADALARQPLILYPARPRPSYADHVLSLFRGQGLQPRVVQEANELQTALGLVAAGLGITLVPSSVQRLHRDDVRYVPINAPGFVSPVIMSYRSGDTSPYLARAVAWVLQQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="113" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LDU6</accession>
    <name>Q1LDU6_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="17">Assimilatory nitrite reductase (NAD(P)H-binding) large subunit</fullName>
            <ecNumber evidence="17">1.7.1.4</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">nirB</name>
        <name evidence="17" type="ordered locus">Rmet_4818</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="18">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="18">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 11">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Nitrogen metabolism; nitrate reduction (assimilation).</text>
    </comment>
    <dbReference type="EC" id="1.7.1.4" evidence="17"/>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11680.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519243.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4818"/>
    <dbReference type="EnsemblBacteria" id="ABF11680">
        <property type="protein sequence ID" value="ABF11680"/>
        <property type="gene ID" value="Rmet_4818"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4818"/>
    <dbReference type="eggNOG" id="COG1251">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003291_0_0_4"/>
    <dbReference type="OMA" id="IVIGHGM"/>
    <dbReference type="OrthoDB" id="1128731at2"/>
    <dbReference type="UniPathway" id="UPA00653"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
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</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="86" xmlns="http://uniprot.org/uniprot">
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        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <source>
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    </evidence>
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        <source>
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</entry>
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        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
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            <dbReference type="PubMed" id="20463976"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF11625.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="94" mass="10505" checksum="1E9AE2A7B8AA5451" modified="2006-05-30" version="1">MIRSLSDKTTAAIYCGQSVHTLPAHIQADALRKLAILDAASSLPDMVAPPANRLEPVLGKRRGHWRVRIKGPWHLCFRFINGEAWDVGIAEYEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="104" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LE44</accession>
    <name>Q1LE44_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Putative homoserine O-acetyltransferase (MetX-like)</fullName>
            <ecNumber evidence="6">2.3.1.31</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Rmet_4720</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="7">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="7">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EC" id="2.3.1.31" evidence="6"/>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11582.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519154.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4720"/>
    <dbReference type="ESTHER" id="ralme-q1le44">
        <property type="family name" value="Homoserine_transacetylase"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF11582">
        <property type="protein sequence ID" value="ABF11582"/>
        <property type="gene ID" value="Rmet_4720"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4720"/>
    <dbReference type="eggNOG" id="COG2021">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_065993_0_0_4"/>
    <dbReference type="OMA" id="MGGMQTW"/>
    <dbReference type="OrthoDB" id="536745at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004414">
        <property type="term" value="F:homoserine O-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009086">
        <property type="term" value="P:methionine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008220">
        <property type="entry name" value="HAT_MetX-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00561">
        <property type="entry name" value="Abhydrolase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000443">
        <property type="entry name" value="Homoser_Ac_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="SSF53474"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="2" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="2" id="KW-0486">Methionine biosynthesis</keyword>
    <keyword evidence="6" id="KW-0614">Plasmid</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="1 6" id="KW-0808">Transferase</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193413" evidence="4">
        <location>
            <begin position="27"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="domain" description="AB hydrolase-1" evidence="5">
        <location>
            <begin position="76"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="3">
        <location>
            <position position="168"/>
        </location>
    </feature>
    <feature type="active site" evidence="3">
        <location>
            <position position="308"/>
        </location>
    </feature>
    <feature type="active site" evidence="3">
        <location>
            <position position="342"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR000443-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABF11582.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="366" mass="40918" checksum="D7068B00953D91D7" modified="2006-05-30" version="1">MKTTITTVGLKRLAVATLFACGIVHAQSTVPVMQPTEGDFEARDFHFQSGQTLPTVKLHYATLGTPTRGADGKVNNAVLLLHGTTGTGRAYLTPLMQKELFAAGQPLDASRYYIIMPDGIGRGGSSKPSDALRANFPRYGYNDVVEGHYRLLTEGLKVDHLRLILGTSMGGMQTWVWGERHPDMMDALMPIASQPVAMSGRNWLWRRMLIDAIRNDPDWNGGNYTRQPTHWTRTTPVFALMTQSAATLQKAAPTRDQVNQYVDKTVADSRGVDANDYLYWFESSWDYNPEPDLGMIRAPLYAVNFADDMINAVDLGVMQRTVPKVRQGKYVEMPESVNTYGHQTLQHPEVWKPYLVELLKSLPAQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="64" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LE66</accession>
    <name>Q1LE66_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_4698</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11560.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519131.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4698"/>
    <dbReference type="EnsemblBacteria" id="ABF11560">
        <property type="protein sequence ID" value="ABF11560"/>
        <property type="gene ID" value="Rmet_4698"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4698"/>
    <dbReference type="eggNOG" id="COG2834">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_081300_0_0_4"/>
    <dbReference type="OMA" id="GWKVRPF"/>
    <dbReference type="OrthoDB" id="1915733at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193423" evidence="1">
        <location>
            <begin position="23"/>
            <end position="259"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF11560.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="259" mass="28191" checksum="52B2C5C2B51EEFBC" modified="2006-05-30" version="1">MNRNLFVALGLAVLVTPVAAAAADAGRMSAAQIVDRNVAARGGLAAWRAVNTLTMSGELDAGGKKETKLPFVATMKRPHKVRFELRFQDQTAVQVYDGTQGWKVRPFLGRDGAEPFTPAEAKAAAEWAELDGPLVDYARKGSTVELEGTDTVEGHRAYRLRLTTKDRVQRRVWVDASSFLEVKMDGEPRVMDGRPRPVAIYYREYGKENGLMMPHVVETVVVGVKPGHKMKIQRVAVNPPVDDAAFAKPQAAVPGAAGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LE85</accession>
    <name>Q1LE85_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">YCII domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_4679</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the YciI family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11541.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519113.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4679"/>
    <dbReference type="EnsemblBacteria" id="ABF11541">
        <property type="protein sequence ID" value="ABF11541"/>
        <property type="gene ID" value="Rmet_4679"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4679"/>
    <dbReference type="eggNOG" id="COG2350">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_110355_3_1_4"/>
    <dbReference type="OMA" id="HQHVRQA"/>
    <dbReference type="OrthoDB" id="1780176at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011008">
        <property type="entry name" value="Dimeric_a/b-barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005545">
        <property type="entry name" value="YCII"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03795">
        <property type="entry name" value="YCII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54909">
        <property type="entry name" value="SSF54909"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="YCII" evidence="2">
        <location>
            <begin position="1"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007689"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF03795"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF11541.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="98" mass="10944" checksum="A5E9E28B936BCC13" modified="2006-05-30" version="1">MPYLIETFDKPEHQHVRQANRPAHLEFLAENSKLLLACGAKLNDDGSDGGGGIYIVDVDTREAARALIEADPFFTAGLFADIRIVRWRKAYLDGVCRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="96" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LEA9</accession>
    <name>Q1LEA9_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Argininosuccinate lyase</fullName>
            <ecNumber evidence="3">4.3.2.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">argH</name>
        <name evidence="8" type="ordered locus">Rmet_4655</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="9">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="9">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>2-(N(omega)-L-arginino)succinate = fumarate + L-arginine</text>
            <dbReference type="Rhea" id="RHEA:24020"/>
            <dbReference type="ChEBI" id="CHEBI:29806"/>
            <dbReference type="ChEBI" id="CHEBI:32682"/>
            <dbReference type="ChEBI" id="CHEBI:57472"/>
            <dbReference type="EC" id="4.3.2.1"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Amino-acid biosynthesis; L-arginine biosynthesis; L-arginine from L-ornithine and carbamoyl phosphate: step 3/3.</text>
    </comment>
    <dbReference type="EC" id="4.3.2.1" evidence="3"/>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11517.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4655"/>
    <dbReference type="EnsemblBacteria" id="ABF11517">
        <property type="protein sequence ID" value="ABF11517"/>
        <property type="gene ID" value="Rmet_4655"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4655"/>
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        <property type="taxonomic scope" value="Bacteria"/>
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    <dbReference type="HOGENOM" id="CLU_027272_3_5_4"/>
    <dbReference type="OMA" id="YTNGVAA"/>
    <dbReference type="UniPathway" id="UPA00068">
        <property type="reaction ID" value="UER00114"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004056">
        <property type="term" value="F:argininosuccinate lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042450">
        <property type="term" value="P:arginine biosynthetic process via ornithine"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01359">
        <property type="entry name" value="Argininosuccinate_lyase"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Arg_succ_lyase_C"/>
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    <dbReference type="InterPro" id="IPR009049">
        <property type="entry name" value="Argininosuccinate_lyase"/>
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        <property type="entry name" value="Fumarase/histidase_N"/>
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    <dbReference type="InterPro" id="IPR000362">
        <property type="entry name" value="Fumarate_lyase_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022761">
        <property type="entry name" value="Fumarate_lyase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008948">
        <property type="entry name" value="L-Aspartase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43814">
        <property type="entry name" value="PTHR43814"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ASL_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00206">
        <property type="entry name" value="Lyase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00149">
        <property type="entry name" value="FUMRATELYASE"/>
    </dbReference>
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        <property type="entry name" value="SSF48557"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="4" id="KW-0055">Arginine biosynthesis</keyword>
    <keyword evidence="8" id="KW-0456">Lyase</keyword>
    <keyword evidence="8" id="KW-0614">Plasmid</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193377" description="Argininosuccinate lyase" evidence="5">
        <location>
            <begin position="34"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="domain" description="Lyase_1" evidence="6">
        <location>
            <begin position="108"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="domain" description="ASL_C2" evidence="7">
        <location>
            <begin position="378"/>
            <end position="453"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000985"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012338"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022571"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF14698"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ABF11517.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="508" mass="55084" checksum="3EE01996DD1282DF" modified="2006-05-30" version="1">MPTPRLHSRVKSLARLTALAMLMMSVGPQAAFAAGGANHDAFFWLGEINKASTVINTDEGLLDRSQAPRVAGGIATVLANGDKPGGQRPSTVITFEPLMIQAAGQEVTLIHAGRSSQDMLATVRAAMLRDEVLSLADQLNKTSATIVKLARQHAETIVPNYTNGVAAQPNSYGHYLLGINAGLERDAQRLREAYQRIDRSPMGTTVLNGTGWPLNRTRMASYLGFSALVDNAYDASQISAVDLPVEVGAIVTSIALHTGSFIEDVMTQYAQPRPWILLQEGGGNTYVSSAMPQKRNPGLLNSTREKASTAITLAMGPVIRAHNLPPGMSDAKDAVTNGQMVQSAITTLKGLDRIMTALVIDRPRALDELDSDWTASQELADELMRKYKLPFRVGHHFASNLVDYAKAHDIKPSAFPYAEAKRIYAEAVRDYGGGEFPMSEAEFRAALDPVAIVKRRVTVGGPQPAEMERMLKASEGQLAQQDAWIRARRAAIDGSLGRLDRDFEKLLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="62" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LEE3</accession>
    <name>Q1LEE3_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_4621</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11483.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4621"/>
    <dbReference type="EnsemblBacteria" id="ABF11483">
        <property type="protein sequence ID" value="ABF11483"/>
        <property type="gene ID" value="Rmet_4621"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4621"/>
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    </dbReference>
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    <dbReference type="OMA" id="NAISWVV"/>
    <dbReference type="OrthoDB" id="1932180at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="entry name" value="UCP028770"/>
    </dbReference>
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        <property type="entry name" value="DUF3302"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193115" evidence="2">
        <location>
            <begin position="23"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="32"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="75"/>
            <end position="97"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF11483.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="130" mass="14663" checksum="8D5B2E6A6BC79A86" modified="2006-05-30" version="1">MKLRPYLLAGILLLASTEPAFASALRGKTLETVANAISWVVLIFVPMAGIYLFWMLHIWPEKIAHRKRHPQKEAIHSLCLLSLFFGGLLWPLAWLWAHTKPVFYRAAYGTDQAPEDTEAPGEIRAGKEPI</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="70" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LF21</accession>
    <name>Q1LF21_CUPMC</name>
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        <submittedName>
            <fullName evidence="1">Putative beta-hydroxyacyl-acyl carrier protein (ACP)-dehydratase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_4390</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11255.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4390"/>
    <dbReference type="EnsemblBacteria" id="ABF11255">
        <property type="protein sequence ID" value="ABF11255"/>
        <property type="gene ID" value="Rmet_4390"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4390"/>
    <dbReference type="eggNOG" id="COG4706">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
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    <dbReference type="OMA" id="GMGVFEC"/>
    <dbReference type="OrthoDB" id="1960828at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="CDD" id="cd01289">
        <property type="entry name" value="FabA_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029069">
        <property type="entry name" value="HotDog_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016776">
        <property type="entry name" value="Hydroxyacyl-ACP_deHydtase_prd"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF020565">
        <property type="entry name" value="3Ho_Ac_ACP_DH_prd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54637">
        <property type="entry name" value="SSF54637"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF11255.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="157" mass="16641" checksum="B40C9F47B6EB3218" modified="2006-05-30" version="1">MRTPAPISTLPPVGELVPHDGAMRLIDELLAADDTQCVARATVRSTQLFVDDAGMPAWIGIEYMAQTIAAWAGMQARGKGCKPGPGFLLGSRRYASDVPAFPVGSVLTIRARLEMTGDDGLSLFACTLDMGGQEVARATVSVYRPADAQAFLEGQQT</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LF51</accession>
    <name>Q1LF51_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Thiamine pyrophosphate enzyme-like TPP binding region</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">oxc</name>
        <name evidence="6" type="ordered locus">Rmet_4360</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="7">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="7">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the TPP enzyme family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11225.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4360"/>
    <dbReference type="EnsemblBacteria" id="ABF11225">
        <property type="protein sequence ID" value="ABF11225"/>
        <property type="gene ID" value="Rmet_4360"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4360"/>
    <dbReference type="eggNOG" id="COG0028">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013748_3_3_4"/>
    <dbReference type="OMA" id="EFGQWAQ"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030976">
        <property type="term" value="F:thiamine pyrophosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044281">
        <property type="term" value="P:small molecule metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029035">
        <property type="entry name" value="DHS-like_NAD/FAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012000">
        <property type="entry name" value="Thiamin_PyroP_enz_cen_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012001">
        <property type="entry name" value="Thiamin_PyroP_enz_TPP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045229">
        <property type="entry name" value="TPP_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011766">
        <property type="entry name" value="TPP_enzyme-bd_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18968">
        <property type="entry name" value="PTHR18968"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02775">
        <property type="entry name" value="TPP_enzyme_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00205">
        <property type="entry name" value="TPP_enzyme_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02776">
        <property type="entry name" value="TPP_enzyme_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52467">
        <property type="entry name" value="SSF52467"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="SSF52518"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0614">Plasmid</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0786">Thiamine pyrophosphate</keyword>
    <feature type="domain" description="TPP_enzyme_N" evidence="5">
        <location>
            <begin position="85"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="domain" description="TPP_enzyme_M" evidence="3">
        <location>
            <begin position="275"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="domain" description="TPP_enzyme_C" evidence="4">
        <location>
            <begin position="469"/>
            <end position="621"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007812"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU362132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF02775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF02776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABF11225.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="640" mass="68982" checksum="C7C675BF700CB972" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LF99</accession>
    <name>Q1LF99_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transcriptional regulator, BadM/Rrf2 family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_4312</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11177.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518801.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4312"/>
    <dbReference type="EnsemblBacteria" id="ABF11177">
        <property type="protein sequence ID" value="ABF11177"/>
        <property type="gene ID" value="Rmet_4312"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4312"/>
    <dbReference type="eggNOG" id="COG1959">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_107144_1_0_4"/>
    <dbReference type="OMA" id="CAIHAVM"/>
    <dbReference type="OrthoDB" id="1794390at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000944">
        <property type="entry name" value="Tscrpt_reg_Rrf2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33221">
        <property type="entry name" value="PTHR33221"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02082">
        <property type="entry name" value="Rrf2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00738">
        <property type="entry name" value="rrf2_super"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51197">
        <property type="entry name" value="HTH_RRF2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="169"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="169"/>
            <end position="183"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF11177.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="191" mass="21030" checksum="8E38B2452E760AF8" modified="2006-05-30" version="1">MSFISTGVEYGLHCLLYLSRSQASVETASVRDLAELQGIPADYLAKLFTKLAKANLVVATEGIRGGFSLARPADKITVHDVVVAIDGDKPLFDCLEIRDRCAVFDEAPPAWATRGVCSIHAVMQSAEKAMRHELRQHTLLDIANRAIDKAPNSYGTQVVQWLTARSVNRRGEKSEESLPPARKSVRTRNRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LFV3</accession>
    <name>Q1LFV3_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Acetone carboxylase alpha subunit (Or Hydantoinase B/oxoprolinase)</fullName>
            <ecNumber evidence="2">3.5.2.14</ecNumber>
            <ecNumber evidence="2">6.4.1.6</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">acxB</name>
        <name evidence="2" type="ordered locus">Rmet_4106</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EC" id="3.5.2.14" evidence="2"/>
    <dbReference type="EC" id="6.4.1.6" evidence="2"/>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10973.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518601.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4106"/>
    <dbReference type="EnsemblBacteria" id="ABF10973">
        <property type="protein sequence ID" value="ABF10973"/>
        <property type="gene ID" value="Rmet_4106"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4106"/>
    <dbReference type="eggNOG" id="COG0146">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020413_2_0_4"/>
    <dbReference type="OMA" id="KYRGGCG"/>
    <dbReference type="OrthoDB" id="1018930at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018710">
        <property type="term" value="F:acetone carboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047423">
        <property type="term" value="F:N-methylhydantoinase (ATP-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003692">
        <property type="entry name" value="Hydantoinase_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045079">
        <property type="entry name" value="Oxoprolinase_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11365">
        <property type="entry name" value="PTHR11365"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02538">
        <property type="entry name" value="Hydantoinase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0436">Ligase</keyword>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Hydantoinase_B" evidence="1">
        <location>
            <begin position="50"/>
            <end position="629"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF10973.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="774" mass="86983" checksum="C50F0246ADF8C567" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-10-07" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LFW8</accession>
    <name>Q1LFW8_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Flavine mononucleotide (FMN) oxidoreductase containing old yellow enzyme (OYE)-related FMN binding domain, group 2 (OYE_like_2_FMN)</fullName>
            <ecNumber evidence="2">1.6.99.1</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_4091</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EC" id="1.6.99.1" evidence="2"/>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10958.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4091"/>
    <dbReference type="EnsemblBacteria" id="ABF10958">
        <property type="protein sequence ID" value="ABF10958"/>
        <property type="gene ID" value="Rmet_4091"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4091"/>
    <dbReference type="eggNOG" id="COG1902">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012153_6_2_4"/>
    <dbReference type="OMA" id="ARMPLMV"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003959">
        <property type="term" value="F:NADPH dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018548">
        <property type="term" value="F:pentaerythritol trinitrate reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001155">
        <property type="entry name" value="OxRdtase_FMN_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00724">
        <property type="entry name" value="Oxidored_FMN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Oxidored_FMN" evidence="1">
        <location>
            <begin position="11"/>
            <end position="342"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00724"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF10958.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="420" mass="45887" checksum="B630BB25B53984F3" modified="2006-05-30" version="1">MRKCLRTPAMKLFTPLTLPNGAVIPNRLAKAAMEENMADADHAPSDALLRLYEAWAAGGAGLMLTGNVMVDHRAMTGPNGVVLENDAHLDRFRQWARIARAHGAHVWMQINHPGRQMPAALGQTTLAPSAVPLELGALSKQFPVPREMTAADIDDVRELFVRSAQLAEQSGFTGVQIHAAHGYLLSQFLSPLANRRQDQWGGPIENRARLLLDIVRAVRQAVSPAFVVSVKLNSADFQRGGFSPDDAKRVVELLNPLGVDLVELSGGSYEAPAMQGQARDGRTLEREAYFLAFARDIAAIAQMPLMVTGGIRRRAVADQVVDSGIAMVGIATALSIDPNLPRDWLAGKNNAPALRPITWKNKVLGSLANMAVVKFQLKRLSQGRGTHPRVSPLRALLMQQMANACQTRRYKRWMMGRELG</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="57" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LG35</accession>
    <name>Q1LG35_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_4023</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10891.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518530.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4023"/>
    <dbReference type="EnsemblBacteria" id="ABF10891">
        <property type="protein sequence ID" value="ABF10891"/>
        <property type="gene ID" value="Rmet_4023"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4023"/>
    <dbReference type="HOGENOM" id="CLU_2104931_0_0_4"/>
    <dbReference type="OMA" id="ICQQGQC"/>
    <dbReference type="OrthoDB" id="2043129at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193506" evidence="2">
        <location>
            <begin position="23"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="42"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="42"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF10891.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="135" mass="13630" checksum="3CF7C83B895E44CB" modified="2006-05-30" version="1">MSLRSILFIAAVTITWNSPAAAAGCQYDMQCKGDRICQQGQCMTPDSDDSDAATSKGQSGQSGQPAQPVQPAPPAQSAMRLAPASTTASAPRNCCTVAGKLRLTTPQSGDAALMVGDACQGLTATGKPVPGTACN</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LG52</accession>
    <name>Q1LG52_CUPMC</name>
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        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Rmet_4006</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
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            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="9">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="9">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10874.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518513.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4006"/>
    <dbReference type="EnsemblBacteria" id="ABF10874">
        <property type="protein sequence ID" value="ABF10874"/>
        <property type="gene ID" value="Rmet_4006"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4006"/>
    <dbReference type="eggNOG" id="COG4461">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_098273_1_0_4"/>
    <dbReference type="OrthoDB" id="1366507at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.128.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009739">
        <property type="entry name" value="LprI_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018660">
        <property type="entry name" value="MliC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036328">
        <property type="entry name" value="MliC_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07007">
        <property type="entry name" value="LprI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09864">
        <property type="entry name" value="MliC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF141488">
        <property type="entry name" value="SSF141488"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0564">Palmitate</keyword>
    <keyword evidence="8" id="KW-0614">Plasmid</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193104" evidence="5">
        <location>
            <begin position="24"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="domain" description="LprI" evidence="6">
        <location>
            <begin position="30"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="domain" description="MliC" evidence="7">
        <location>
            <begin position="136"/>
            <end position="203"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF07007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF09864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ABF10874.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="211" mass="22873" checksum="B65FD91367162789" modified="2006-05-30" version="1">MPIRLSVPTVALAMLGTTSVAMAASPSFDCGNAKGQVEQLICKDDTLARLDRETTRLYGMALDARSLPAPQKKTLLAEQRGWIKGRNDCWKASDAGVCVAASYLERIHELRHAYAGARTQDDKGISRGPFDIRCPDVKAVIAGTFVQTEPGFAYLRWLDQQLVLTQTPAASGARYTATTANGEAVFWDKGPDAQLTLPGKADMNCRVGIRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LG80</accession>
    <name>Q1LG80_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="9">ABC-type dipeptide/oligopeptide/nickel transport system, ATPase component</fullName>
            <ecNumber evidence="9">3.6.3.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">livG4</name>
        <name evidence="9" type="ordered locus">Rmet_3978</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="10">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="10">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EC" id="3.6.3.-" evidence="9"/>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10846.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518486.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3978"/>
    <dbReference type="EnsemblBacteria" id="ABF10846">
        <property type="protein sequence ID" value="ABF10846"/>
        <property type="gene ID" value="Rmet_3978"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3978"/>
    <dbReference type="eggNOG" id="COG0411">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_2_4"/>
    <dbReference type="OMA" id="VEHDMPM"/>
    <dbReference type="OrthoDB" id="1179151at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032823">
        <property type="entry name" value="BCA_ABC_TP_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12399">
        <property type="entry name" value="BCA_ABC_TP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="1" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-0614">Plasmid</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-1278">Translocase</keyword>
    <feature type="domain" description="ABC transporter" evidence="8">
        <location>
            <begin position="2"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="34"/>
            <end position="41"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ABF10846.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="254" mass="27541" checksum="C5C57790312F8570" modified="2006-05-30" version="1">MLEIRNLTKKFGGLTAVHDVSVKFETGHINAIIGPNGAGKTTFFNLIAGTHAPTSGQILLKGQDVAGLRADQIARLGVARTFQATSLFDRATVLDNLIVGHRLRTKSGLADVLFNTSRLREEEKLCREKAEAALDFVGLSHLAHELAADITQEARKRVAFALALATDPDLLLLDEPAGGVNPEETVGLAELIRKMVRHGKTVCLIEHKMDMIMRLADKIMVLNYGEKIAEGTPAQIQQDPHVIEAYLGADHVAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LGZ8</accession>
    <name>Q1LGZ8_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_3706</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PrpF family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10578.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518226.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3706"/>
    <dbReference type="EnsemblBacteria" id="ABF10578">
        <property type="protein sequence ID" value="ABF10578"/>
        <property type="gene ID" value="Rmet_3706"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3706"/>
    <dbReference type="eggNOG" id="COG2828">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026443_2_1_4"/>
    <dbReference type="OMA" id="TCIDVGN"/>
    <dbReference type="OrthoDB" id="1328531at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016853">
        <property type="term" value="F:isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007400">
        <property type="entry name" value="PrpF_protein"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43709">
        <property type="entry name" value="PTHR43709"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04303">
        <property type="entry name" value="PrpF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0413">Isomerase</keyword>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF10578.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="382" mass="39927" checksum="5F8D8B2F9FEACF81" modified="2006-05-30" version="1">MTPCLASDLRAIPCTLMRGGTSRGPFFLASDLPEDVPARDRVLLAAMGSPDPRQIDGLGGAHPLTSKACIVSRGTPGHADLDFTFAQLQPNADTVDTTPNCGNMLAAVLPFAIERGLIAPQGDITRARVLTRNTGMLCDITIQTPGGVPRFSGNARIDGVPGAAAPIVIDFLDTAGSVCHAMLPTGNAIDVLTINDPVRGGSRLIAATLIDNGMPMVLMRAEDLDRTGYESVAALNADHELKQRIEAARLVAGELMGLGDVSDKSYPKMCLLARPRQGGSIATRCFIPRVCHDAIGVLAAITVGTACVLPGTVASDIAVTSVGPLHAISVEHPTGEFTVELETDPAKPGSIVRSALIRTARPIMRGEVLVPTSAWPLSDELP</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-09-02" modified="2022-08-03" version="100" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LH94</accession>
    <name>MNME_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">tRNA modification GTPase MnmE</fullName>
            <ecNumber evidence="1">3.6.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">mnmE</name>
        <name evidence="1" type="synonym">trmE</name>
        <name type="ordered locus">Rmet_3610</name>
    </gene>
    <organism>
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Exhibits a very high intrinsic GTPase hydrolysis rate. Involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA-cmnm(5)s(2)U34.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </cofactor>
        <text evidence="1">Binds 1 potassium ion per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer. Heterotetramer of two MnmE and two MnmG subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily. TrmE GTPase family.</text>
    </comment>
    <dbReference type="EC" id="3.6.-.-" evidence="1"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10482.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518024.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q1LH94"/>
    <dbReference type="SMR" id="Q1LH94"/>
    <dbReference type="STRING" id="266264.Rmet_3610"/>
    <dbReference type="EnsemblBacteria" id="ABF10482">
        <property type="protein sequence ID" value="ABF10482"/>
        <property type="gene ID" value="Rmet_3610"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3610"/>
    <dbReference type="eggNOG" id="COG0486">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019624_4_1_4"/>
    <dbReference type="OMA" id="CEIQCHG"/>
    <dbReference type="OrthoDB" id="263682at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006400">
        <property type="term" value="P:tRNA modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd04164">
        <property type="entry name" value="trmE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.430">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1360.120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00379">
        <property type="entry name" value="GTPase_MnmE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031168">
        <property type="entry name" value="G_TrmE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018948">
        <property type="entry name" value="GTP-bd_TrmE_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004520">
        <property type="entry name" value="GTPase_MnmE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027368">
        <property type="entry name" value="MnmE_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025867">
        <property type="entry name" value="MnmE_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027266">
        <property type="entry name" value="TrmE/GcvT_dom1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01926">
        <property type="entry name" value="MMR_HSR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12631">
        <property type="entry name" value="MnmE_helical"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10396">
        <property type="entry name" value="TrmE_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00326">
        <property type="entry name" value="GTP1OBG"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00450">
        <property type="entry name" value="mnmE_trmE_thdF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51709">
        <property type="entry name" value="G_TRME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0342">GTP-binding</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0630">Potassium</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0819">tRNA processing</keyword>
    <feature type="chain" id="PRO_0000345881" description="tRNA modification GTPase MnmE">
        <location>
            <begin position="1"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="domain" description="TrmE-type G">
        <location>
            <begin position="220"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="24"/>
        </location>
        <ligand>
            <name>(6S)-5-formyl-5,6,7,8-tetrahydrofolate</name>
            <dbReference type="ChEBI" id="CHEBI:57457"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="81"/>
        </location>
        <ligand>
            <name>(6S)-5-formyl-5,6,7,8-tetrahydrofolate</name>
            <dbReference type="ChEBI" id="CHEBI:57457"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="124"/>
        </location>
        <ligand>
            <name>(6S)-5-formyl-5,6,7,8-tetrahydrofolate</name>
            <dbReference type="ChEBI" id="CHEBI:57457"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="230"/>
            <end position="235"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="230"/>
        </location>
        <ligand>
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="234"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="249"/>
            <end position="255"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="249"/>
        </location>
        <ligand>
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="251"/>
        </location>
        <ligand>
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="254"/>
        </location>
        <ligand>
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="255"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="274"/>
            <end position="277"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="378"/>
            <end position="380"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="475"/>
        </location>
        <ligand>
            <name>(6S)-5-formyl-5,6,7,8-tetrahydrofolate</name>
            <dbReference type="ChEBI" id="CHEBI:57457"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00379"/>
        </source>
    </evidence>
    <sequence length="475" mass="50990" checksum="D4C10D11115792A3" modified="2006-05-30" version="1">MTVSQIPIAAIATAPGRGGIGVVRVSGPDVGAVMRAVCGRALQPRHATYLPFLDARGNVIDHGLALYFPAPNSYTGEEVLELQGHGGPVVMQMLLSRCLEAGKDIGLRVAEPGEFTRRAFLNDKLDLAQAEAVADLIEASTEAAARSAARSMEGEFSKAIHALVEKVIHLRMLVEATLDFPEEEIDFLEASNARGQLTRIREDLAGVLKQARQGSLLREGLSVVLAGQPNVGKSSLLNALAGSDLAIVTPIAGTTRDRVRETIQIDGIPLHIIDTAGLRDDAADEVERIGIERTWEAIRHADIVLHLIDAADYIEHGISETDDHIDDRLSGQLPPGSPIVRVINKIDLAPSVGAMGFGGNRPHVVAANGPNPTEIWISARTGAGIDLMRSELLRLIGWQSGNEGAFLARERHLIALRNAESHLELAEASASQHAQALDLFAEELRLAQDHLNSITGEFTSDDLLGTIFTRFCIGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="95" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LHI5</accession>
    <name>Q1LHI5_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Leucine/isoleucine/valine transporter subunit, ATP-binding component of ABC superfamily</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">livF5</name>
        <name evidence="9" type="ordered locus">Rmet_3519</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10391.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008648867.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3519"/>
    <dbReference type="EnsemblBacteria" id="ABF10391">
        <property type="protein sequence ID" value="ABF10391"/>
        <property type="gene ID" value="Rmet_3519"/>
    </dbReference>
    <dbReference type="GeneID" id="60822624"/>
    <dbReference type="KEGG" id="rme:Rmet_3519"/>
    <dbReference type="eggNOG" id="COG0410">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_2_4"/>
    <dbReference type="OMA" id="MDHGQMV"/>
    <dbReference type="OrthoDB" id="1475841at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 7 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="1" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-1278">Translocase</keyword>
    <feature type="domain" description="ABC transporter" evidence="8">
        <location>
            <begin position="9"/>
            <end position="241"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ABF10391.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="241" mass="26366" checksum="63E015DEC6D7A334" modified="2006-05-30" version="1">MANDNTPALEIKDLHAWYGESHILHGVDLTVNRGEVVTLLGRNGAGRTTTMRAIMGLTGARKGSIKVNGHETIGLPTHKIAHFGIGYCPEERAIFSSLSCEENLLLPPMLKGANAGKGMSEAEIYEMFPNLKERRQSQGTRLSGGEQQMLAVGRILRTGANLLLLDEISEGLAPVIVQALARMILMLKKKGYTVVMVEQNFRFAAPLADRFYVMEHGAIVERFAANELQAKMPVLNELLGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="96" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LHQ8</accession>
    <name>Q1LHQ8_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Transcriptional regulator, LysR-family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_3446</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the LysR transcriptional regulatory family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10318.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3446"/>
    <dbReference type="EnsemblBacteria" id="ABF10318">
        <property type="protein sequence ID" value="ABF10318"/>
        <property type="gene ID" value="Rmet_3446"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3446"/>
    <dbReference type="eggNOG" id="COG0583">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039613_16_2_4"/>
    <dbReference type="OMA" id="FIAVVEH"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005119">
        <property type="entry name" value="LysR_subst-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000847">
        <property type="entry name" value="Tscrpt_reg_HTH_LysR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00126">
        <property type="entry name" value="HTH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03466">
        <property type="entry name" value="LysR_substrate"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50931">
        <property type="entry name" value="HTH_LYSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH lysR-type" evidence="2">
        <location>
            <begin position="30"/>
            <end position="87"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF10318.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="331" mass="36715" checksum="2B2E47E75A2E26C7" modified="2006-05-30" version="1">MGVIAPRGCAIWLRCLTMCRQETFSWAGMDKLKQIEAFIAVVEHGSMAAAALTQNVTPVMIGRRINALEARIGVKLLHRSTRRIVVTEQGAAFMEQCKKALADLDRAEMLIAEGKHKATGHLIVSAPAAFGRKHVAPHAPAFLAANPEVQISFNLTDRVVDLVREGYDVGIRIGGAIDPNFVAIKLATNKRVVCGTPAYFARHGVPHTLEDLEKHNCLAFNLQGGQQRGWYFQQNGKTVTVRVNGNLDCNDGELLHRWTGESLGLGWRSTWEILPQLESGELITVLDEYALPDYDILAVYPQQRPVPAKIRFFIEHLKQAFAKPGYWSGRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LHZ0</accession>
    <name>Q1LHZ0_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">IstB-like ATP binding protein ISRme4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">tnpB</name>
        <name evidence="3" type="ordered locus">Rmet_0484</name>
        <name evidence="4" type="ordered locus">Rmet_3364</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="submission" date="2006-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of the chromosome of Ralstonia metallidurans CH34.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Copeland A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Barry K."/>
                <person name="Detter J.C."/>
                <person name="Glavina del Rio T."/>
                <person name="Hammon N."/>
                <person name="Israni S."/>
                <person name="Dalin E."/>
                <person name="Tice H."/>
                <person name="Martinez M."/>
                <person name="Goltsman E."/>
                <person name="Pitluck S."/>
                <person name="Schmutz J."/>
                <person name="Larimer F."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Kim E."/>
                <person name="Mergeay M."/>
                <person name="Benotmane M.A."/>
                <person name="Vallaeys T."/>
                <person name="Michaux A."/>
                <person name="Monchy S."/>
                <person name="Dunn J."/>
                <person name="McCorkle S."/>
                <person name="Taghavi S."/>
                <person name="van der Lelie D."/>
                <person name="Richardson P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CH34</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2010-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Benotmane R."/>
                <person name="Lapidus A."/>
                <person name="McCorkle S."/>
                <person name="Monchy S."/>
                <person name="Taghavi S."/>
                <person name="van der Lelie N."/>
                <person name="Dunn J."/>
                <person name="Leys N."/>
                <person name="Mergeay M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07370.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10236.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515356.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0484"/>
    <dbReference type="EnsemblBacteria" id="ABF07370">
        <property type="protein sequence ID" value="ABF07370"/>
        <property type="gene ID" value="Rmet_0484"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF10236">
        <property type="protein sequence ID" value="ABF10236"/>
        <property type="gene ID" value="Rmet_3364"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0484"/>
    <dbReference type="KEGG" id="rme:Rmet_3364"/>
    <dbReference type="eggNOG" id="COG1484">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_062999_1_2_4"/>
    <dbReference type="OMA" id="AYEKRSV"/>
    <dbReference type="OrthoDB" id="1792718at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028350">
        <property type="entry name" value="DNAC/ATP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002611">
        <property type="entry name" value="IstB_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01695">
        <property type="entry name" value="IstB_IS21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF003073">
        <property type="entry name" value="DNAC_TnpB_IstB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA" evidence="2">
        <location>
            <begin position="97"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="238"/>
            <end position="261"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF07370.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF10236.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="261" mass="29059" checksum="BAA61F9CAA3F1ABB" modified="2006-05-30" version="1">MQHEAMMEMLKSLKLHGMAHALGDLAAQDSPAYQSAVAILSRLLKAETTEREVRSLAYQMKVARFPAYRDLTGFDFSCSEANEALVRQLHRCEFLESAHNVVLVGGPGTGKTHLGTAIGVQAVEHHHRRVRFFSTVELVNALELEKVSGKPGHLATRLMYADLVILDELGYLPFSQVGGALLFHLISKLYERTSLIITTNLSFGEWSSVFGDAKMTTALLDRLTHHCHIVETGNDSYRFKHSTTQPKKEKRTAKQPAQSDT</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="55" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LI22</accession>
    <name>Q1LI22_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_3332</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10204.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3332"/>
    <dbReference type="EnsemblBacteria" id="ABF10204">
        <property type="protein sequence ID" value="ABF10204"/>
        <property type="gene ID" value="Rmet_3332"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3332"/>
    <dbReference type="HOGENOM" id="CLU_1729874_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="20"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="117"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="137"/>
            <end position="151"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF10204.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="151" mass="17023" checksum="E82887BA24666889" modified="2006-05-30" version="1">MRAGNLRDVRKSIDDGRYGWPERFSARQPKPMQPPFPVSRISPSHRIVRGLAIAGSLMLASTRAAADEALWLDLSASQPGATVEEHRRQRFEEHYDCLSGRGNARLCERLEDQARQGQMDVLRQSASPAPLTPPVDPDEFRRQDIPLPGRR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-01-15" modified="2022-05-25" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LI49</accession>
    <name>RL5_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">50S ribosomal protein L5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rplE</name>
        <name type="ordered locus">Rmet_3305</name>
    </gene>
    <organism>
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">This is 1 of the proteins that binds and probably mediates the attachment of the 5S RNA into the large ribosomal subunit, where it forms part of the central protuberance. In the 70S ribosome it contacts protein S13 of the 30S subunit (bridge B1b), connecting the 2 subunits; this bridge is implicated in subunit movement. Contacts the P site tRNA; the 5S rRNA and some of its associated proteins might help stabilize positioning of ribosome-bound tRNAs.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the 50S ribosomal subunit; part of the 5S rRNA/L5/L18/L25 subcomplex. Contacts the 5S rRNA and the P site tRNA. Forms a bridge to the 30S subunit in the 70S ribosome.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL5 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10177.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008642941.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q1LI49"/>
    <dbReference type="SMR" id="Q1LI49"/>
    <dbReference type="STRING" id="266264.Rmet_3305"/>
    <dbReference type="EnsemblBacteria" id="ABF10177">
        <property type="protein sequence ID" value="ABF10177"/>
        <property type="gene ID" value="Rmet_3305"/>
    </dbReference>
    <dbReference type="GeneID" id="60826612"/>
    <dbReference type="KEGG" id="rme:Rmet_3305"/>
    <dbReference type="eggNOG" id="COG0094">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_061015_2_1_4"/>
    <dbReference type="OMA" id="ERMYAFL"/>
    <dbReference type="OrthoDB" id="1456375at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1440.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01333_B">
        <property type="entry name" value="Ribosomal_L5_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002132">
        <property type="entry name" value="Ribosomal_L5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020930">
        <property type="entry name" value="Ribosomal_L5_bac-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031309">
        <property type="entry name" value="Ribosomal_L5_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020929">
        <property type="entry name" value="Ribosomal_L5_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022803">
        <property type="entry name" value="Ribosomal_L5_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031310">
        <property type="entry name" value="Ribosomal_L5_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11994">
        <property type="entry name" value="PTHR11994"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00281">
        <property type="entry name" value="Ribosomal_L5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00673">
        <property type="entry name" value="Ribosomal_L5_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002161">
        <property type="entry name" value="Ribosomal_L5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55282">
        <property type="entry name" value="SSF55282"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00358">
        <property type="entry name" value="RIBOSOMAL_L5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <keyword id="KW-0820">tRNA-binding</keyword>
    <feature type="chain" id="PRO_1000052806" description="50S ribosomal protein L5">
        <location>
            <begin position="1"/>
            <end position="180"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01333"/>
        </source>
    </evidence>
    <sequence length="180" mass="20272" checksum="EA8E55437E5DEBD6" modified="2006-05-30" version="1">MTARLQEFYKEQVVPALMKQFGYKSVMEVPRFTKITLNMGLGEAINDKKVIELAVGDLTKIAGQKPVVTKAKKAIAGFKIRQGYPIGAMVTLRGERMFEFLDRFVTVALPRVRDFRGVSGKSFDGRGNYNIGVKEQIIFPEIEYDKIDALRGLNISITTTAKNDEEAKALLNAFKFPFRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="68" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LI83</accession>
    <name>Q1LI83_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Tfp pilus assembly protein PilN</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">pilN</name>
        <name evidence="3" type="ordered locus">Rmet_3271</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10143.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517743.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3271"/>
    <dbReference type="EnsemblBacteria" id="ABF10143">
        <property type="protein sequence ID" value="ABF10143"/>
        <property type="gene ID" value="Rmet_3271"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3271"/>
    <dbReference type="eggNOG" id="COG3166">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_081304_1_0_4"/>
    <dbReference type="OMA" id="DRNLPVH"/>
    <dbReference type="OrthoDB" id="1673053at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007813">
        <property type="entry name" value="PilN"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05137">
        <property type="entry name" value="PilN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="28"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="192"/>
            <end position="214"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF10143.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="214" mass="23551" checksum="55947A45173D6623" modified="2006-05-30" version="1">MSTTTNSIPSVNLLPYHEARKAARRKKVYTMLGGAAGAGALVVLIGGFYIDSQIDAVAARNQVLKTENASMDATISRVNSLKKDIEALLDRQRAIESLQAQRNLPVQLLEELVRQVPEGVYLSSLKQTGDKFVMSGVAQSNERVSELLRNISQVTWLDQADLVESKAINMTNNLREQRRLFEFSMRFSYRAPEVPGDKKKGASDAAGTPAGRKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LIA1</accession>
    <name>Q1LIA1_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Putative transporter subunit: ATP-binding component of ABC superfamily</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">yadG</name>
        <name evidence="9" type="ordered locus">Rmet_3253</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10125.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008643041.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3253"/>
    <dbReference type="EnsemblBacteria" id="ABF10125">
        <property type="protein sequence ID" value="ABF10125"/>
        <property type="gene ID" value="Rmet_3253"/>
    </dbReference>
    <dbReference type="GeneID" id="60825577"/>
    <dbReference type="KEGG" id="rme:Rmet_3253"/>
    <dbReference type="eggNOG" id="COG1131">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_2_4"/>
    <dbReference type="OMA" id="CEGYRRP"/>
    <dbReference type="OrthoDB" id="1926714at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 7 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="1" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-1278">Translocase</keyword>
    <feature type="domain" description="ABC transporter" evidence="8">
        <location>
            <begin position="4"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="36"/>
            <end position="43"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ABF10125.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="316" mass="35111" checksum="1816CFAF2602AAE2" modified="2006-05-30" version="1">MKAIEITDVRKRYRNLQALKGVSFSVERGEFFGLLGPNGAGKTTLISILAGLNRANSGRVSVLGHDVVDDYRMARRMLGVVPQELVFDPFFTVRETLRLQSGYFGLTRNDDWIDEIMANLDLTNKADSNMRQLSGGMKRRVLVAQALVHRPPVIVLDEPTAGVDVELRQTLWKFISRLNREGHTIILTTHYLEEAEALCDRIAMLKFGEVVALDRTSNLLTQFAGLQLVLTLSRGTLPPALEALRTPSNPGERAHRLRLSGYEAVEPILAALREAGCEIEDMEISKADLEDVFVQIMRREGQMPGALPNTAMEAAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="92" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LII2</accession>
    <name>Q1LII2_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Nitrite reductase (Cytochrome cd1) (Cytochrome oxidase) (Hydroxylamine reductase)</fullName>
            <ecNumber evidence="7">1.7.2.1</ecNumber>
            <ecNumber evidence="7">1.7.99.1</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">nirS</name>
        <name evidence="7" type="ordered locus">Rmet_3172</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EC" id="1.7.2.1" evidence="7"/>
    <dbReference type="EC" id="1.7.99.1" evidence="7"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10044.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517656.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3172"/>
    <dbReference type="EnsemblBacteria" id="ABF10044">
        <property type="protein sequence ID" value="ABF10044"/>
        <property type="gene ID" value="Rmet_3172"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3172"/>
    <dbReference type="eggNOG" id="COG2010">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025262_0_0_4"/>
    <dbReference type="OMA" id="MPNWGTS"/>
    <dbReference type="OrthoDB" id="302683at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050418">
        <property type="term" value="F:hydroxylamine reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050421">
        <property type="term" value="F:nitrite reductase (NO-forming) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.760.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.140.10.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009056">
        <property type="entry name" value="Cyt_c-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036909">
        <property type="entry name" value="Cyt_c-like_dom_sf"/>
    </dbReference>
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        <property type="entry name" value="Cyt_cd1_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011048">
        <property type="entry name" value="Haem_d1_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13442">
        <property type="entry name" value="Cytochrome_CBB3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46626">
        <property type="entry name" value="SSF46626"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SSF51004"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51007">
        <property type="entry name" value="CYTC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 4" id="KW-0349">Heme</keyword>
    <keyword evidence="3 4" id="KW-0408">Iron</keyword>
    <keyword evidence="2 4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193199" evidence="5">
        <location>
            <begin position="26"/>
            <end position="549"/>
        </location>
    </feature>
    <feature type="domain" description="Cytochrome c" evidence="6">
        <location>
            <begin position="40"/>
            <end position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABF10044.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="549" mass="60769" checksum="42925C728CDA747D" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="109" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LII8</accession>
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        <submittedName>
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        </submittedName>
    </protein>
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        <name evidence="7" type="ordered locus">Rmet_3166</name>
    </gene>
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        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10038.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517648.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3166"/>
    <dbReference type="EnsemblBacteria" id="ABF10038">
        <property type="protein sequence ID" value="ABF10038"/>
        <property type="gene ID" value="Rmet_3166"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3166"/>
    <dbReference type="eggNOG" id="COG3604">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_125_1_4"/>
    <dbReference type="OMA" id="TWFEPDS"/>
    <dbReference type="OrthoDB" id="123059at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="AAA+_ATPase"/>
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        <property type="entry name" value="GAF-like_dom_sf"/>
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        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
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        <property type="entry name" value="HTH_Fis"/>
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        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Sigma_54_int_dom_ATP-bd_1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00158">
        <property type="entry name" value="Sigma54_activat"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00065">
        <property type="entry name" value="GAF"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Sigma-54 factor interaction" evidence="6">
        <location>
            <begin position="189"/>
            <end position="418"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50045"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABF10038.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="525" mass="57121" checksum="0E1A80BC42A45E4A" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="64" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LIW3</accession>
    <name>Q1LIW3_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_3041</name>
    </gene>
    <organism evidence="2 3">
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        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09913.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3041"/>
    <dbReference type="EnsemblBacteria" id="ABF09913">
        <property type="protein sequence ID" value="ABF09913"/>
        <property type="gene ID" value="Rmet_3041"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3041"/>
    <dbReference type="eggNOG" id="COG2333">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_044335_0_0_4"/>
    <dbReference type="OMA" id="AEVAIWE"/>
    <dbReference type="OrthoDB" id="1796527at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="SSF56281"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="368"/>
            <end position="397"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF09913.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="397" mass="44423" checksum="A30EEE96485C28DE" modified="2006-05-30" version="1">MPTTITFFPVDNGDMTLIKFGDLDATTLLIDVNIRQDADDPDGEARDVAKDLRERLKKDENGRPYVDAFLLSHPDQDHCRGLKRHFYLGPLDKYPDDKKDDKDKKIVIREMWSSPIVFRRASKTHTLSDDAKAFNTEARRRVQLNRDKNFAVGNGDRIQIMGEDIDGKTDDLTSIVRKVDTRFSTINGKSSAFFSAFLLAPLDAQDDEEEEECLVKNQSSVILNITLAADAQTPDGAKFLTGGDAEVFIWNRQWQRHKAEADVLEYDIMQAPHHCSWHSLSYDSWSDYREKAKLDADARKALSQTRDGAVIVASCKPIADDDSDPPCIRAKREYVTIVDEAKGEFYCTGEYPSEKSVEPLVFTVTAQGVQPPSKKESGSKAAAVVTSARTPMPHGAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-12-02" version="56" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LJ13</accession>
    <name>Q1LJ13_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">DNA-binding protein transcriptional activator, homocysteine-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">metR</name>
        <name evidence="2" type="ordered locus">Rmet_2990</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09863.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2990"/>
    <dbReference type="EnsemblBacteria" id="ABF09863">
        <property type="protein sequence ID" value="ABF09863"/>
        <property type="gene ID" value="Rmet_2990"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2990"/>
    <dbReference type="eggNOG" id="COG0583">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1599900_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005119">
        <property type="entry name" value="LysR_subst-bd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03466">
        <property type="entry name" value="LysR_substrate"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="LysR_substrate" evidence="1">
        <location>
            <begin position="14"/>
            <end position="152"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF09863.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="166" mass="18395" checksum="5348B31B14453DB1" modified="2006-05-30" version="1">MGHFWVEINTLFKPGLKFEPVFDYEQVLVVAKDHPLASTAYVKPQQLTREVLISYPVDIERLDIYSQFLLPAGVTPKRHKAIETTDIMVQMVASGRGVAALPRWLVEEYAARMDVVPVRLGARGIAKQIFLGAREADTAIDYVRAFVELARQPATATTAIAQGAKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LJ32</accession>
    <name>Q1LJ32_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DNA polymerase III subunit delta</fullName>
            <ecNumber evidence="1">2.7.7.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">holA</name>
        <name evidence="7" type="ordered locus">Rmet_2971</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) = diphosphate + DNA(n+1)</text>
            <dbReference type="Rhea" id="RHEA:22508"/>
            <dbReference type="Rhea" id="RHEA-COMP:17339"/>
            <dbReference type="Rhea" id="RHEA-COMP:17340"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61560"/>
            <dbReference type="ChEBI" id="CHEBI:173112"/>
            <dbReference type="EC" id="2.7.7.7"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.7.7.7" evidence="1"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09844.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517507.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2971"/>
    <dbReference type="EnsemblBacteria" id="ABF09844">
        <property type="protein sequence ID" value="ABF09844"/>
        <property type="gene ID" value="Rmet_2971"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2971"/>
    <dbReference type="eggNOG" id="COG1466">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_044694_0_0_4"/>
    <dbReference type="OMA" id="RIPTGKP"/>
    <dbReference type="OrthoDB" id="1199768at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009360">
        <property type="term" value="C:DNA polymerase III complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003887">
        <property type="term" value="F:DNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008921">
        <property type="entry name" value="DNA_pol3_clamp-load_cplx_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032780">
        <property type="entry name" value="DNA_pol3_delt_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010372">
        <property type="entry name" value="DNA_pol3_delta_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005790">
        <property type="entry name" value="DNA_polIII_delta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14840">
        <property type="entry name" value="DNA_pol3_delt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06144">
        <property type="entry name" value="DNA_pol3_delta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48019">
        <property type="entry name" value="SSF48019"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01128">
        <property type="entry name" value="holA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0239">DNA-directed DNA polymerase</keyword>
    <keyword evidence="7" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="DNA_pol3_delta" evidence="5">
        <location>
            <begin position="24"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="domain" description="DNA_pol3_delt_C" evidence="6">
        <location>
            <begin position="223"/>
            <end position="321"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00017703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00024632"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF06144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF14840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABF09844.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="356" mass="38654" checksum="D61100F9BCB87777" modified="2006-05-30" version="1">MQLKLDGLEAHLRQAQAKGLAPLYVVHGDEHLLVLEAVDRLRRAAREAGFSERDVLVAERGFQWGQVIEAQQSMSLFGDRKIVELRIPSGKPGKDGGEALRAVAAQPSPDVVMLVTLPRLDFATTKSAWFQALESAGVSIKVDAVDRTRLPAWVGERLGLQQQRVEPGEPGRRALQFIAEKVEGNLLAAHQEIQKLGLLYPPGALTFDQVQDAVLNVARYDVFKLSEAMLGGDVPRLVRMLEGLRGEGEATVLVLWALTEEIRVLSKVRQGLAGGKPAGVLMRELRVWGPRERLVPQAAQRLSMPQLEAALAMAARLDRQVKGLSDLPGAGGAAGALPAEPWDGLMQLALMIARPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="97" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LJ74</accession>
    <name>Q1LJ74_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Glycolate oxidase iron-sulfur subunit</fullName>
            <ecNumber evidence="4">1.1.99.14</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">glcF</name>
        <name evidence="6" type="ordered locus">Rmet_2929</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Component of a complex that catalyzes the oxidation of glycolate to glyoxylate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>(R)-lactate + A = AH2 + pyruvate</text>
            <dbReference type="Rhea" id="RHEA:15089"/>
            <dbReference type="ChEBI" id="CHEBI:13193"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:16004"/>
            <dbReference type="ChEBI" id="CHEBI:17499"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>A + glycolate = AH2 + glyoxylate</text>
            <dbReference type="Rhea" id="RHEA:21264"/>
            <dbReference type="ChEBI" id="CHEBI:13193"/>
            <dbReference type="ChEBI" id="CHEBI:17499"/>
            <dbReference type="ChEBI" id="CHEBI:29805"/>
            <dbReference type="ChEBI" id="CHEBI:36655"/>
            <dbReference type="EC" id="1.1.99.14"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="4">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="4">Binds 2 [4Fe-4S] clusters.</text>
    </comment>
    <dbReference type="EC" id="1.1.99.14" evidence="4"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09802.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517474.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2929"/>
    <dbReference type="EnsemblBacteria" id="ABF09802">
        <property type="protein sequence ID" value="ABF09802"/>
        <property type="gene ID" value="Rmet_2929"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2929"/>
    <dbReference type="eggNOG" id="COG0247">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023081_0_0_4"/>
    <dbReference type="OMA" id="VYQDACH"/>
    <dbReference type="OrthoDB" id="1951488at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003973">
        <property type="term" value="F:(S)-2-hydroxy-acid oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047809">
        <property type="term" value="F:D-2-hydroxy-acid dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019154">
        <property type="term" value="F:glycolate dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1060.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004017">
        <property type="entry name" value="Cys_rich_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012257">
        <property type="entry name" value="Glc_ox_4Fe-4S"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009051">
        <property type="entry name" value="Helical_ferredxn"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32479:SF17">
        <property type="entry name" value="PTHR32479:SF17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02754">
        <property type="entry name" value="CCG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12838">
        <property type="entry name" value="Fer4_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000139">
        <property type="entry name" value="Glc_ox_4Fe-4S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="4" id="KW-0249">Electron transport</keyword>
    <keyword evidence="2 4" id="KW-0408">Iron</keyword>
    <keyword evidence="3 4" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="1 4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="5">
        <location>
            <begin position="16"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="5">
        <location>
            <begin position="66"/>
            <end position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRNR" id="PIRNR000139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABF09802.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="423" mass="46350" checksum="58B0038CE676C48A" modified="2006-05-30" version="1">MQTTLADFLRNTPEGEEAKSIVGKCVHCGFCTATCPTYQLLGDELDGPRGRIYLMKQVLEGNPVTESTRLHLDRCLTCRNCESTCPSGVTYGHLVDIGRKIVDDRLEAQGIKRPAKERIVRWVLREGLTRPALFGPALRLGQIVRPWLPQMLRNKVPASEPVAGTWPRTEHPRKMLLLDGCVQPAMSPNINAATARVFDKLGVQLVVAREAGCCGAIRFHNGDHPGGLDNMRANIDAWWPHIEAGAEAIVMTASGCGAMVKDYGHLLRNDPTYASRAARVSAMTRDLSELLPDFTDELHRLAGAVPRDSRRVAWHPPCTLQHGQQIRGKVEALLTRLGVDVRLCADSHLCCGSAGTYSVLQPELSYKLRDDKLAKLQATSPESIVTANIGCITHLQSGTETPVMHWVELVDRMLAPVVAAQAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LJB5</accession>
    <name>Q1LJB5_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative bacterial luciferase-likee oxidoreductase protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_2888</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">To bacterial alkanal monooxygenase alpha and beta chains.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09761.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517441.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2888"/>
    <dbReference type="EnsemblBacteria" id="ABF09761">
        <property type="protein sequence ID" value="ABF09761"/>
        <property type="gene ID" value="Rmet_2888"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2888"/>
    <dbReference type="eggNOG" id="COG2141">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027853_9_0_4"/>
    <dbReference type="OMA" id="HHNMPSV"/>
    <dbReference type="OrthoDB" id="1003376at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011251">
        <property type="entry name" value="Luciferase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036661">
        <property type="entry name" value="Luciferase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019949">
        <property type="entry name" value="Luciferase_OxRdatse_group-1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00296">
        <property type="entry name" value="Bac_luciferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51679">
        <property type="entry name" value="SSF51679"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03558">
        <property type="entry name" value="oxido_grp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Bac_luciferase" evidence="2">
        <location>
            <begin position="21"/>
            <end position="306"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007789"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF09761.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="339" mass="37296" checksum="ACDC35F35AC4264E" modified="2006-05-30" version="1">MSQPRPKLRLSVLDQSPVIAGHTARDAVAATVELAQMADDLGYTRYWCAEHHGLQGVCNPAPEVMLARLGSATKRIRVGSGGVMLPYYSPFKVAEQFRMLEALFPNRVDLGVGRAPGGDMRTAQAVAMGQYNRGDQFPQQVQDLAWLLRGEVPPDHLAEGVLLQPVIDTKPELWVLGSSDFGGALAARLGLRFAFAHFINAHSGHLVTQQYREAFEPGYEDKPYSAAAIFVICADTEQEAATLERAVDIRRLQMAYGVNAPIPSLAQAADFTPTERDRMIIERERPRTICGTPEQVAERMLALKDRFAADELVVLSVTASYKARLRTYQLLAEAFDLAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LJF2</accession>
    <name>Q1LJF2_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Formiminoglutamate deiminase</fullName>
            <ecNumber evidence="3">3.5.3.13</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">hutF</name>
        <name evidence="3" type="ordered locus">Rmet_2851</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EC" id="3.5.3.13" evidence="3"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09724.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517409.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2851"/>
    <dbReference type="EnsemblBacteria" id="ABF09724">
        <property type="protein sequence ID" value="ABF09724"/>
        <property type="gene ID" value="Rmet_2851"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2851"/>
    <dbReference type="eggNOG" id="COG0402">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012358_3_0_4"/>
    <dbReference type="OMA" id="MCPTTER"/>
    <dbReference type="OrthoDB" id="1592010at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050416">
        <property type="term" value="F:formimidoylglutamate deiminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006680">
        <property type="entry name" value="Amidohydro-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010252">
        <property type="entry name" value="HutF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011059">
        <property type="entry name" value="Metal-dep_hydrolase_composite"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43608">
        <property type="entry name" value="PTHR43608"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01979">
        <property type="entry name" value="Amidohydro_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="SSF51556"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02022">
        <property type="entry name" value="hutF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Amidohydro-rel" evidence="2">
        <location>
            <begin position="52"/>
            <end position="420"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF09724.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="469" mass="50279" checksum="88DEB1E4A5FEFD78" modified="2006-05-30" version="1">MTAVTSLFAPHALLPGGWARNVRLQWDAHGVLTSVTTDVAPAPGESQAAGPVLPGMPNLHSHAFQRGFAGLTEYRSVSQTADPAGGDSFWSWRALMYRFALRLSPDALEAIATQLYIEMLSAGYTSVCEFHYVHHDVDGKPYADPAEMSLRLLRAAQTAGIGMTLLPVLYQTAGFGGKPPQDDQRRFLNDTDAMLALLEKLAPHCAAYGARLGLAPHSLRAVPPHSLAQAVAHLHAMDATAPVHIHIAEQQKEVDECLATTGVRPVQWLLDHAPVDARWCLVHATHMDWDERRRLAHSGAVAGICPTTEANLGDGVFDAAPYLAQNGAWGIGSDSHASVSVAEELRLFEYGQRLAMQRRNVLASARHPQVADLVWLDAVAGGARASARPVAGLAVGQRADFVVLDGNHPTLAGLDGSRALATHVFASHGHETLAEVRTGGMCRVQHGVHPQQAEAGRLFAEARARLLAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="61" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LJN7</accession>
    <name>Q1LJN7_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_2766</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09639.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517337.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2766"/>
    <dbReference type="EnsemblBacteria" id="ABF09639">
        <property type="protein sequence ID" value="ABF09639"/>
        <property type="gene ID" value="Rmet_2766"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2766"/>
    <dbReference type="eggNOG" id="ENOG502ZBP6">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_114505_1_0_4"/>
    <dbReference type="OMA" id="RPESFKA"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021736">
        <property type="entry name" value="DUF3305"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11749">
        <property type="entry name" value="DUF3305"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="149"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="155"/>
            <end position="178"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF09639.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="178" mass="20542" checksum="517977CCD27BE420" modified="2006-05-30" version="1">MDQPAVPEPEALPAGRPSVTMAVVMRRVALTNRWQPWKWELDAVLPDLGEFGTAPKRLDADEHGEHWAHPGYAVTLYRDEGEGYYLNLTSTAPCWFVLWRMAEEPDEDGETRPVPVTVSLSYNEAGRWLDAGENVENVPLEPEQRAWLQAFTDQHYRPEPKKRRRPESFKAPEERARY</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-01-15" modified="2022-08-03" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LJV9</accession>
    <name>RISB_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">6,7-dimethyl-8-ribityllumazine synthase</fullName>
            <shortName evidence="1">DMRL synthase</shortName>
            <shortName evidence="1">LS</shortName>
            <shortName evidence="1">Lumazine synthase</shortName>
            <ecNumber evidence="1">2.5.1.78</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">ribH</name>
        <name type="ordered locus">Rmet_2694</name>
    </gene>
    <organism>
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the formation of 6,7-dimethyl-8-ribityllumazine by condensation of 5-amino-6-(D-ribitylamino)uracil with 3,4-dihydroxy-2-butanone 4-phosphate. This is the penultimate step in the biosynthesis of riboflavin.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(2S)-2-hydroxy-3-oxobutyl phosphate + 5-amino-6-(D-ribitylamino)uracil = 6,7-dimethyl-8-(1-D-ribityl)lumazine + H(+) + 2 H2O + phosphate</text>
            <dbReference type="Rhea" id="RHEA:26152"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15934"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58201"/>
            <dbReference type="ChEBI" id="CHEBI:58830"/>
            <dbReference type="EC" id="2.5.1.78"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; riboflavin biosynthesis; riboflavin from 2-hydroxy-3-oxobutyl phosphate and 5-amino-6-(D-ribitylamino)uracil: step 1/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the DMRL synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.78" evidence="1"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09567.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517267.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q1LJV9"/>
    <dbReference type="SMR" id="Q1LJV9"/>
    <dbReference type="STRING" id="266264.Rmet_2694"/>
    <dbReference type="EnsemblBacteria" id="ABF09567">
        <property type="protein sequence ID" value="ABF09567"/>
        <property type="gene ID" value="Rmet_2694"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2694"/>
    <dbReference type="eggNOG" id="COG0054">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_089358_1_2_4"/>
    <dbReference type="OMA" id="CQGVTQG"/>
    <dbReference type="OrthoDB" id="1680292at2"/>
    <dbReference type="UniPathway" id="UPA00275">
        <property type="reaction ID" value="UER00404"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009349">
        <property type="term" value="C:riboflavin synthase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000906">
        <property type="term" value="F:6,7-dimethyl-8-ribityllumazine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009231">
        <property type="term" value="P:riboflavin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd09209">
        <property type="entry name" value="Lumazine_synthase-I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.960">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00178">
        <property type="entry name" value="Lumazine_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034964">
        <property type="entry name" value="LS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002180">
        <property type="entry name" value="LS/RS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036467">
        <property type="entry name" value="LS/RS_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21058">
        <property type="entry name" value="PTHR21058"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00885">
        <property type="entry name" value="DMRL_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52121">
        <property type="entry name" value="SSF52121"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00114">
        <property type="entry name" value="lumazine-synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0686">Riboflavin biosynthesis</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_1000040498" description="6,7-dimethyl-8-ribityllumazine synthase">
        <location>
            <begin position="1"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="1">
        <location>
            <position position="90"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="24"/>
        </location>
        <ligand>
            <name>5-amino-6-(D-ribitylamino)uracil</name>
            <dbReference type="ChEBI" id="CHEBI:15934"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="58"/>
            <end position="60"/>
        </location>
        <ligand>
            <name>5-amino-6-(D-ribitylamino)uracil</name>
            <dbReference type="ChEBI" id="CHEBI:15934"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="82"/>
            <end position="84"/>
        </location>
        <ligand>
            <name>5-amino-6-(D-ribitylamino)uracil</name>
            <dbReference type="ChEBI" id="CHEBI:15934"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="87"/>
            <end position="88"/>
        </location>
        <ligand>
            <name>(2S)-2-hydroxy-3-oxobutyl phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58830"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="115"/>
        </location>
        <ligand>
            <name>5-amino-6-(D-ribitylamino)uracil</name>
            <dbReference type="ChEBI" id="CHEBI:15934"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="129"/>
        </location>
        <ligand>
            <name>(2S)-2-hydroxy-3-oxobutyl phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58830"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00178"/>
        </source>
    </evidence>
    <sequence length="169" mass="18183" checksum="6851C571BD1F38C9" modified="2006-05-30" version="1">MDHGFYESNLDGEGLRIGIVQARFNEPVCDELREACMAELKKLGVEGEDTLLVTVPGALEVPLALQKMAESGQFDALVALGAVVRGETYHFELVSNESGAGITRVGLDFNVPIANGILTVDTDAQAHARTREKGRDCARAAVEMANLVVALDSLREHGAEDAEDEEDDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LK94</accession>
    <name>Q1LK94_CUPMC</name>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_2555</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
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            <taxon>Betaproteobacteria</taxon>
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        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09432.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517132.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2555"/>
    <dbReference type="EnsemblBacteria" id="ABF09432">
        <property type="protein sequence ID" value="ABF09432"/>
        <property type="gene ID" value="Rmet_2555"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2555"/>
    <dbReference type="HOGENOM" id="CLU_2481053_0_0_4"/>
    <dbReference type="OrthoDB" id="61310at119060"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="55"/>
            <end position="87"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF09432.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="87" mass="9710" checksum="631C214C723B794A" modified="2006-05-30" version="1">MGALILLESLYWRNGALPSQDDLEAHRVARSITPDVWAKVLRLYFPDGRAVHLDDEREKAITKSKASSTNGGKGGRPRKATEKTEDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="51" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LKY7</accession>
    <name>Q1LKY7_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_2310</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
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        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09189.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2310"/>
    <dbReference type="EnsemblBacteria" id="ABF09189">
        <property type="protein sequence ID" value="ABF09189"/>
        <property type="gene ID" value="Rmet_2310"/>
    </dbReference>
    <dbReference type="GeneID" id="61526436"/>
    <dbReference type="KEGG" id="rme:Rmet_2310"/>
    <dbReference type="eggNOG" id="ENOG5031791">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_186899_0_0_4"/>
    <dbReference type="OMA" id="QMRHIAS"/>
    <dbReference type="OrthoDB" id="1755471at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF09189.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="92" mass="10471" checksum="8A2102EED9540C31" modified="2006-05-30" version="1">MFPDLITPASDFEHQLGACVNAMSQEDAIGQILVFERMSGTLHMRHIASADLVDTDVDDYEMVVFDGGNTSGDTWKHVFFPRQREHYFVYQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="65" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LLA6</accession>
    <name>Q1LLA6_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">DUF4149 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Rmet_2191</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
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        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09070.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516898.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2191"/>
    <dbReference type="EnsemblBacteria" id="ABF09070">
        <property type="protein sequence ID" value="ABF09070"/>
        <property type="gene ID" value="Rmet_2191"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2191"/>
    <dbReference type="eggNOG" id="ENOG5031AKJ">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_136732_1_0_4"/>
    <dbReference type="OMA" id="QFGMLHG"/>
    <dbReference type="OrthoDB" id="1744022at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025423">
        <property type="entry name" value="DUF4149"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13664">
        <property type="entry name" value="DUF4149"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2 4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="16"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="56"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="91"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="136"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="domain" description="DUF4149" evidence="5">
        <location>
            <begin position="19"/>
            <end position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF13664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABF09070.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="163" mass="18029" checksum="C62C660234ABB3AE" modified="2006-05-30" version="1">MFSSSYSNLPPLPHRLFLLLTVIWAGSLWTIGYLVAPTLFSVLPSRETAGMIAGHLFRYEAILGMTVGVLQLVLCNVLIRRGAERYRSLRWIVLTMLVCVLAGYFGVQPFMEGLKVKAQALNLGVSESPYKAEFGMLHGVSSVIYLVQSVLALVLVWRASAPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="65" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LLF9</accession>
    <name>Q1LLF9_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">4-Amino-4-deoxy-L-arabinose transferase or related glycosyltransferase of PMT family</fullName>
            <ecNumber evidence="2">2.4.1.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">arnT</name>
        <name evidence="2" type="ordered locus">Rmet_2138</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.4.1.-" evidence="2"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09017.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2138"/>
    <dbReference type="TCDB" id="9.B.142.2.10">
        <property type="family name" value="the integral membrane glycosyltransferase family 39 (gt39) family"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF09017">
        <property type="protein sequence ID" value="ABF09017"/>
        <property type="gene ID" value="Rmet_2138"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2138"/>
    <dbReference type="eggNOG" id="COG1807">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_034283_0_0_4"/>
    <dbReference type="OMA" id="DWLMPNI"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="86"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="111"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="151"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="179"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="207"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="246"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="295"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="332"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="355"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="385"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="424"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="458"/>
            <end position="481"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF09017.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="600" mass="66123" checksum="66B8F38754645CD1" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-01-15" modified="2022-05-25" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LLG9</accession>
    <name>Y2128_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Nucleoid-associated protein Rmet_2128</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">Rmet_2128</name>
    </gene>
    <organism>
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Binds to DNA and alters its conformation. May be involved in regulation of gene expression, nucleoid organization and DNA protection.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Nucleoid</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the YbaB/EbfC family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09007.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008647477.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q1LLG9"/>
    <dbReference type="SMR" id="Q1LLG9"/>
    <dbReference type="STRING" id="266264.Rmet_2128"/>
    <dbReference type="EnsemblBacteria" id="ABF09007">
        <property type="protein sequence ID" value="ABF09007"/>
        <property type="gene ID" value="Rmet_2128"/>
    </dbReference>
    <dbReference type="GeneID" id="60821291"/>
    <dbReference type="KEGG" id="rme:Rmet_2128"/>
    <dbReference type="eggNOG" id="COG0718">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_140930_0_0_4"/>
    <dbReference type="OMA" id="MGNMMKQ"/>
    <dbReference type="OrthoDB" id="1883809at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043590">
        <property type="term" value="C:bacterial nucleoid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1310.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00274">
        <property type="entry name" value="DNA_YbaB_EbfC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036894">
        <property type="entry name" value="YbaB-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004401">
        <property type="entry name" value="YbaB/EbfC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33449">
        <property type="entry name" value="PTHR33449"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02575">
        <property type="entry name" value="YbaB_DNA_bd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004555">
        <property type="entry name" value="UCP004555"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82607">
        <property type="entry name" value="SSF82607"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00103">
        <property type="entry name" value="DNA_YbaB_EbfC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0238">DNA-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_1000003806" description="Nucleoid-associated protein Rmet_2128">
        <location>
            <begin position="1"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="86"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="108" mass="11754" checksum="0614D38C4FF362D4" modified="2006-05-30" version="1">MMKGQLAGLMKQAQQMQENMKKMQEQLAQIEVEGQSGAGLVKVVMTCKNDVKRVTIDPSLLADDKDLLEDLVAAAFNDAVRKAEATTQEKMGSMTSGLPLPPGFKLPF</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-01-15" modified="2022-05-25" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LLK8</accession>
    <name>TAL_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Transaldolase</fullName>
            <ecNumber evidence="2">2.2.1.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">tal</name>
        <name type="ordered locus">Rmet_2089</name>
    </gene>
    <organism>
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Transaldolase is important for the balance of metabolites in the pentose-phosphate pathway.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>D-glyceraldehyde 3-phosphate + D-sedoheptulose 7-phosphate = beta-D-fructose 6-phosphate + D-erythrose 4-phosphate</text>
            <dbReference type="Rhea" id="RHEA:17053"/>
            <dbReference type="ChEBI" id="CHEBI:16897"/>
            <dbReference type="ChEBI" id="CHEBI:57483"/>
            <dbReference type="ChEBI" id="CHEBI:57634"/>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
            <dbReference type="EC" id="2.2.1.2"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Carbohydrate degradation; pentose phosphate pathway; D-glyceraldehyde 3-phosphate and beta-D-fructose 6-phosphate from D-ribose 5-phosphate and D-xylulose 5-phosphate (non-oxidative stage): step 2/3.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the transaldolase family. Type 1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.2.1.2" evidence="2"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08968.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516802.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q1LLK8"/>
    <dbReference type="SMR" id="Q1LLK8"/>
    <dbReference type="STRING" id="266264.Rmet_2089"/>
    <dbReference type="EnsemblBacteria" id="ABF08968">
        <property type="protein sequence ID" value="ABF08968"/>
        <property type="gene ID" value="Rmet_2089"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2089"/>
    <dbReference type="eggNOG" id="COG0176">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047470_0_1_4"/>
    <dbReference type="OMA" id="KFGYKTL"/>
    <dbReference type="OrthoDB" id="784333at2"/>
    <dbReference type="UniPathway" id="UPA00115">
        <property type="reaction ID" value="UER00414"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004801">
        <property type="term" value="F:transaldolase activity"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006098">
        <property type="term" value="P:pentose-phosphate shunt"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00957">
        <property type="entry name" value="Transaldolase_TalAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00492">
        <property type="entry name" value="Transaldolase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001585">
        <property type="entry name" value="TAL/FSA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004730">
        <property type="entry name" value="Transaldolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018225">
        <property type="entry name" value="Transaldolase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10683">
        <property type="entry name" value="PTHR10683"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10683:SF18">
        <property type="entry name" value="PTHR10683:SF18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00923">
        <property type="entry name" value="TAL_FSA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00874">
        <property type="entry name" value="talAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01054">
        <property type="entry name" value="TRANSALDOLASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00958">
        <property type="entry name" value="TRANSALDOLASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0570">Pentose shunt</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0704">Schiff base</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_1000014517" description="Transaldolase">
        <location>
            <begin position="1"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="active site" description="Schiff-base intermediate with substrate" evidence="2">
        <location>
            <position position="126"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00492"/>
        </source>
    </evidence>
    <sequence length="318" mass="34919" checksum="22FB34901FE6586B" modified="2006-05-30" version="1">MNELEQLRQFTTVVADTGDFQLMKQYKPQDATTNPSLIFKAVQKPEYRPLLEQAVRDHHGNSGLDGVMDQLLIAFGCEILAIVPGRVSTEVDARLSFDTEATVAKARHLIGLYEQRGVARERVLIKIASTWEGIRAAEVLQRENIRCNMTLLFSLAQAVACAEAGAQLISPFVGRILDWYKKQAGEKWDPVANGGENDPGVRSVRQIYDYYKKFGYATEVMGASFRGTDQILSLAGCDLLTISPELLEQLAGGQSAVALKLSVEQAQAGNVARIAADEPAFRWQLNEDAMATEKLSEGIRLFAADAVKLEKLVGELAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-10-07" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LLV7</accession>
    <name>Q1LLV7_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_1990</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08869.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1990"/>
    <dbReference type="EnsemblBacteria" id="ABF08869">
        <property type="protein sequence ID" value="ABF08869"/>
        <property type="gene ID" value="Rmet_1990"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1990"/>
    <dbReference type="HOGENOM" id="CLU_2702161_0_0_4"/>
    <dbReference type="OrthoDB" id="785253at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF08869.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="73" mass="8555" checksum="052F0A275F991DEE" modified="2006-05-30" version="1">MGTITQRVRKDGSIWHTAQIRLKQGGATMFTEAKTLDRKQAAKSWLKKRERKLAQLGQRRPHELRLYPRLQNA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="67" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LM29</accession>
    <name>Q1LM29_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ordered locus">Rmet_1918</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TMEM175 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08797.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516650.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1918"/>
    <dbReference type="EnsemblBacteria" id="ABF08797">
        <property type="protein sequence ID" value="ABF08797"/>
        <property type="gene ID" value="Rmet_1918"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1918"/>
    <dbReference type="eggNOG" id="COG3548">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_090238_1_0_4"/>
    <dbReference type="OMA" id="TGWMGEN"/>
    <dbReference type="OrthoDB" id="1399320at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005267">
        <property type="term" value="F:potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010617">
        <property type="entry name" value="TMEM175"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06736">
        <property type="entry name" value="TMEM175"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0407">Ion channel</keyword>
    <keyword evidence="9" id="KW-0406">Ion transport</keyword>
    <keyword evidence="10 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0630">Potassium</keyword>
    <keyword evidence="6" id="KW-0631">Potassium channel</keyword>
    <keyword evidence="4" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="43"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="72"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="105"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="152"/>
            <end position="182"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006920"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ABF08797.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="199" mass="22453" checksum="6E8166AD55EF0AA2" modified="2006-05-30" version="1">MTINKSRLESFSDGVISVSLTLMLYQIQLPAEFNWTGVRAEAPHFFGYVLSFIYVGIYWNNHHHLFQMVRGINGKVMWANLNLLFWLTMIPITTTWTGKSEFSEIPTALYAFVLFMCAISYWILQRVIMASEGQGSMLAGAIGKDRKGRVSLVVYALAIVVSFFSSIAATLILIAMAAFWFLPDSRIERYLLGQGGRDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LM46</accession>
    <name>Q1LM46_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transcriptional regulator, XRE family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_1901</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08780.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516634.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1901"/>
    <dbReference type="EnsemblBacteria" id="ABF08780">
        <property type="protein sequence ID" value="ABF08780"/>
        <property type="gene ID" value="Rmet_1901"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1901"/>
    <dbReference type="eggNOG" id="COG3655">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_090969_1_0_4"/>
    <dbReference type="OMA" id="FRFYLRG"/>
    <dbReference type="OrthoDB" id="1607334at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00093">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001387">
        <property type="entry name" value="Cro/C1-type_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010982">
        <property type="entry name" value="Lambda_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13443">
        <property type="entry name" value="HTH_26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00530">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47413">
        <property type="entry name" value="SSF47413"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50943">
        <property type="entry name" value="HTH_CROC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH cro/C1-type" evidence="1">
        <location>
            <begin position="11"/>
            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF08780.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="246" mass="27797" checksum="C7C7D08739AC73E0" modified="2006-05-30" version="1">MSEVEQLVATIKRQLKAQGLTYRDVAQALALSEASVKRLFASERLNLDRLAQLATLLGFTLAELTQEAAAALPVLRRLTREQEAQLVLDRKLLLVAVCALNHWSAEEIVAAYQITRAECVERLLVLDRMGLITLLPGDRIRLRVMRDFDWLPDGPIRHFFREEGLNDFLDSPFNAPGQTMEFAHAMLTGPALEQLRLELQRLRARVALLHDESASAPLAQRRGIGMLLATREWEPTGFASLRQRPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-10-07" version="49" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LM90</accession>
    <name>Q1LM90_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_1857</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08736.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1857"/>
    <dbReference type="EnsemblBacteria" id="ABF08736">
        <property type="protein sequence ID" value="ABF08736"/>
        <property type="gene ID" value="Rmet_1857"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1857"/>
    <dbReference type="HOGENOM" id="CLU_148514_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="83"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="126"/>
            <end position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF08736.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="144" mass="15890" checksum="FBB9A6999007CE49" modified="2006-05-30" version="1">MNGVIELFGWFVFNVALPLGAPLALLPLARVPSFFRAHRRGIVWHAIKGGQLLWAVIPMSASACHLLATALEIPGVSDALRPYIWFVMAIHVVVIVAASVLVLFATMDAHQRDVLAHSQAQRIPRSWVWLTCMSGASILWATWL</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LMA7</accession>
    <name>Q1LMA7_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Nucleoside-diphosphate-sugar epimerase</fullName>
            <ecNumber evidence="2">5.1.3.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_1840</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EC" id="5.1.3.-" evidence="2"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08719.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516570.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1840"/>
    <dbReference type="EnsemblBacteria" id="ABF08719">
        <property type="protein sequence ID" value="ABF08719"/>
        <property type="gene ID" value="Rmet_1840"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1840"/>
    <dbReference type="eggNOG" id="COG0451">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007383_19_0_4"/>
    <dbReference type="OMA" id="HWHASPR"/>
    <dbReference type="OrthoDB" id="1180629at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016853">
        <property type="term" value="F:isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001509">
        <property type="entry name" value="Epimerase_deHydtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01370">
        <property type="entry name" value="Epimerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0413">Isomerase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Epimerase" evidence="1">
        <location>
            <begin position="3"/>
            <end position="198"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF08719.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="323" mass="34346" checksum="57F0601AC2AC6444" modified="2006-05-30" version="1">MNVLITGGAGFLGLQLARLLLKRGTLNLDGKAVTIDRLTLLDVVAPQIDDARVRAVTGDLSDPAVLAQAIDRDTGAIFHLAAVVSGQAEADFDLGMRVNLDASRALLETCRQLAHAPRVVFTSSVAVYGGKLPPVVQDDTALNPQSSYGVQKAIGELLLSDYSRRGFVDGRVLRLPTISVRPGKPNAAASSFASGIIREPLSGVAANCPVAPETPLWLLSPRGAIQALVNGIELDGARIGNRRVVNLPGLSVTAAQMVEALRRVAGDAVADRVTWEREERIEKIVGTWPAAWDVSRARELGFESDASFDDVIRAYAEDAAVKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LMI7</accession>
    <name>Q1LMI7_CUPMC</name>
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        <submittedName>
            <fullName evidence="3">Alpha/beta hydrolase fold</fullName>
            <ecNumber evidence="3">3.3.2.9</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_1760</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EC" id="3.3.2.9" evidence="3"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08639.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516492.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="ESTHER" id="ralme-q1lmi7">
        <property type="family name" value="CFTR-inhibitory-factor_Cif"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF08639">
        <property type="protein sequence ID" value="ABF08639"/>
        <property type="gene ID" value="Rmet_1760"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1760"/>
    <dbReference type="eggNOG" id="COG2267">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020336_7_1_4"/>
    <dbReference type="OMA" id="APFWDLY"/>
    <dbReference type="OrthoDB" id="1439833at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033961">
        <property type="term" value="F:cis-stilbene-oxide hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000639">
        <property type="entry name" value="Epox_hydrolase-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00561">
        <property type="entry name" value="Abhydrolase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00111">
        <property type="entry name" value="ABHYDROLASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00412">
        <property type="entry name" value="EPOXHYDRLASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="SSF53474"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193618" evidence="1">
        <location>
            <begin position="21"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="domain" description="AB hydrolase-1" evidence="2">
        <location>
            <begin position="50"/>
            <end position="287"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF08639.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="302" mass="33022" checksum="15B62D1C2B74EA1A" modified="2006-05-30" version="1">MRRLLAAVVFMLAMVQPLQAQVKPFPPGFRHQNIQTEGATLYVRVGGSGPAVVLLHGFGDTGDMWAPLAAELARTHTVVVPDLRGMGLSSHPDGGYDKRTQAGDIRSVLAQLNIDQADVVGHDIGTMVAYAYAARYPDKTKRLIVMDAPVPGVPPWEQIVRMPALWHFSFGGPDAERLVKGRERIYLDRFWNEFAGDPSKIDEATRVHYSRLYAKPGAMHSAFAQFLSIPQDAEDNQKSLATKLTMPVLAIGGAKSFGQNEAIVMRNAATNVKELVIPNAGHWLMEEQPQATVGAIVDFLGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LMK9</accession>
    <name>Q1LMK9_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Cellulose synthase (UDP-forming)</fullName>
            <ecNumber evidence="3">2.4.1.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">Rmet_1738</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>[(1-&gt;4)-beta-D-glucosyl](n) + UDP-alpha-D-glucose = [(1-&gt;4)-beta-D-glucosyl](n+1) + H(+) + UDP</text>
            <dbReference type="Rhea" id="RHEA:19929"/>
            <dbReference type="Rhea" id="RHEA-COMP:10033"/>
            <dbReference type="Rhea" id="RHEA-COMP:10034"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:18246"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
            <dbReference type="EC" id="2.4.1.12"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell inner membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.4.1.12" evidence="3"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08617.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516469.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1738"/>
    <dbReference type="CAZy" id="GT2">
        <property type="family name" value="Glycosyltransferase Family 2"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF08617">
        <property type="protein sequence ID" value="ABF08617"/>
        <property type="gene ID" value="Rmet_1738"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1738"/>
    <dbReference type="eggNOG" id="COG1215">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011907_3_0_4"/>
    <dbReference type="OMA" id="WCLGTIQ"/>
    <dbReference type="OrthoDB" id="724641at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016760">
        <property type="term" value="F:cellulose synthase (UDP-forming) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035438">
        <property type="term" value="F:cyclic-di-GMP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030244">
        <property type="term" value="P:cellulose biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006011">
        <property type="term" value="P:UDP-glucose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003919">
        <property type="entry name" value="Cell_synth_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001173">
        <property type="entry name" value="Glyco_trans_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009875">
        <property type="entry name" value="PilZ_domain"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00535">
        <property type="entry name" value="Glycos_transf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07238">
        <property type="entry name" value="PilZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01439">
        <property type="entry name" value="CELLSNTHASEA"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="SSF53448"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0135">Cellulose biosynthesis</keyword>
    <keyword evidence="9" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="6"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="37"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="73"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="374"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="408"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="438"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="476"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="509"/>
            <end position="529"/>
        </location>
    </feature>
    <feature type="domain" description="Glyco_trans_2-like" evidence="7">
        <location>
            <begin position="119"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="domain" description="PilZ" evidence="8">
        <location>
            <begin position="537"/>
            <end position="631"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004429"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012539"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF07238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ABF08617.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="658" mass="73646" checksum="E032467ABBCD2E6B" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LNC4</accession>
    <name>Q1LNC4_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">TatD-related deoxyribonuclease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_1469</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08352.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516217.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1469"/>
    <dbReference type="EnsemblBacteria" id="ABF08352">
        <property type="protein sequence ID" value="ABF08352"/>
        <property type="gene ID" value="Rmet_1469"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1469"/>
    <dbReference type="eggNOG" id="COG0084">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031506_5_1_4"/>
    <dbReference type="OMA" id="HFHWFKT"/>
    <dbReference type="OrthoDB" id="915852at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016788">
        <property type="term" value="F:hydrolase activity, acting on ester bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01310">
        <property type="entry name" value="TatD_DNAse"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001130">
        <property type="entry name" value="TatD-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01026">
        <property type="entry name" value="TatD_DNase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005902">
        <property type="entry name" value="DNase_TatD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="SSF51556"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF08352.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="249" mass="27580" checksum="368B1B2287645155" modified="2006-05-30" version="1">MIATAGLVDFHCHLDLYPNHAAAVQDAEASGVFTLAVTTTPRAWPRNHELAQRTKHVRAALGLHPQLVAERENEIELWDQHLAETRYVGEVGLDAGPRFFKSLDAQKRVFQHVLQRCAQAGDKIITVHSIRSAKAVLDLVEAHLPPERGKIVLHWFTGTKSEAKRALELGCYFSINAAMLGNERHAPMVQAIPLDRMLTETDGPFTRTGDRPSQPTDVVSVVEELGCLHRLPANDMASLIRDNLRALLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="96" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LNP0</accession>
    <name>Q1LNP0_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">23S rRNA pseudouridine synthase</fullName>
            <ecNumber evidence="5">4.2.1.70</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">rluD</name>
        <name evidence="5" type="ordered locus">Rmet_1353</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the pseudouridine synthase RluA family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.70" evidence="5"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08236.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516117.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1353"/>
    <dbReference type="EnsemblBacteria" id="ABF08236">
        <property type="protein sequence ID" value="ABF08236"/>
        <property type="gene ID" value="Rmet_1353"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1353"/>
    <dbReference type="eggNOG" id="COG0564">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016902_4_0_4"/>
    <dbReference type="OMA" id="AHPSPGW"/>
    <dbReference type="OrthoDB" id="1714699at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004730">
        <property type="term" value="F:pseudouridylate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120159">
        <property type="term" value="F:rRNA pseudouridine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000455">
        <property type="term" value="P:enzyme-directed rRNA pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="CDD" id="cd02869">
        <property type="entry name" value="PseudoU_synth_RluA_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00165">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.290.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020103">
        <property type="entry name" value="PsdUridine_synth_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006225">
        <property type="entry name" value="PsdUridine_synth_RluC/D"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006224">
        <property type="entry name" value="PsdUridine_synth_RluC/D_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006145">
        <property type="entry name" value="PsdUridine_synth_RsuA/RluA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002942">
        <property type="entry name" value="S4_RNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036986">
        <property type="entry name" value="S4_RNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00849">
        <property type="entry name" value="PseudoU_synth_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01479">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00363">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55120">
        <property type="entry name" value="SSF55120"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00005">
        <property type="entry name" value="rluA_subfam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01129">
        <property type="entry name" value="PSI_RLU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0456">Lyase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="S4" evidence="4">
        <location>
            <begin position="69"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="362"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="8"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="372"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="active site" evidence="2">
        <location>
            <position position="190"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010876"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR606225-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABF08236.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="403" mass="44683" checksum="2C391E44693504D6" modified="2006-05-30" version="1">MKNSAKHYSRSPKPETGPERHTESTAVADVDTEEFGDLGEFNDDVPVSPPAASDFVPLYMEVDSASHGERLDKLLARHFSEFSRSRIQQWIEDGAVRVDGEPSRAKASVQMGQKIEVQPQAAPESMAFTAEDVPLDVVYEDDAMLVINKPAGLVVHPAAGNWSGTVLNGLLHRDPSAARLPRAGIVHRLDKETSGLMVVARTLVAQTDLVRQLAARTVKRTYLALVWGETPDDGTIDAPIGRDPRERTRMAIVHTNSGKPSRTHFETLGTVPLGRGKVSMVMCQLETGRTHQIRVHFESIGHPLVGDPVYHKATQRGQRPAIRVPLPVPFTRQALHAYRLGLVHPVTGRKMSWEAEPPEDLQALIDALDFEGHAQDDEEEDDWYEGDDDDQVDHDDHDDHDDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LNR5</accession>
    <name>Q1LNR5_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Toluene hydroxylase P3 protein (Toluene 2-monooxygenase P3 component) (Protein belongs to CMGI-2)</fullName>
            <ecNumber evidence="2">1.14.13.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">tomA3</name>
        <name evidence="2" type="ordered locus">Rmet_1328</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EC" id="1.14.13.-" evidence="2"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08211.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516094.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1328"/>
    <dbReference type="EnsemblBacteria" id="ABF08211">
        <property type="protein sequence ID" value="ABF08211"/>
        <property type="gene ID" value="Rmet_1328"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1328"/>
    <dbReference type="eggNOG" id="COG3350">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_040795_0_0_4"/>
    <dbReference type="OMA" id="HIFRQAP"/>
    <dbReference type="OrthoDB" id="147428at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006725">
        <property type="term" value="P:cellular aromatic compound metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.620.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009078">
        <property type="entry name" value="Ferritin-like_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003430">
        <property type="entry name" value="Phenol_Hydrox"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012348">
        <property type="entry name" value="RNR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007029">
        <property type="entry name" value="YHS_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02332">
        <property type="entry name" value="Phenol_Hydrox"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04945">
        <property type="entry name" value="YHS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47240">
        <property type="entry name" value="SSF47240"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="YHS" evidence="1">
        <location>
            <begin position="411"/>
            <end position="453"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF04945"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF08211.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="519" mass="60983" checksum="3360E827ACA89D9C" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="70" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LNR6</accession>
    <name>Q1LNR6_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Toluene ortho-monooxygenase P4 protein (Toluene ortho-monooxygenase P4 component) (Protein belongs to CMGI-2)</fullName>
            <ecNumber evidence="1">1.14.13.7</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">tomA4</name>
        <name evidence="1" type="ordered locus">Rmet_1327</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EC" id="1.14.13.7" evidence="1"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08210.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516093.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1327"/>
    <dbReference type="EnsemblBacteria" id="ABF08210">
        <property type="protein sequence ID" value="ABF08210"/>
        <property type="gene ID" value="Rmet_1327"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1327"/>
    <dbReference type="eggNOG" id="ENOG5032U7S">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2057138_0_0_4"/>
    <dbReference type="OMA" id="AGINGSC"/>
    <dbReference type="OrthoDB" id="1844114at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018662">
        <property type="term" value="F:phenol 2-monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.560">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006756">
        <property type="entry name" value="Phenol_hydroxylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043010">
        <property type="entry name" value="Phenol_hydroxylase_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04663">
        <property type="entry name" value="Phenol_monoox"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF08210.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="118" mass="13079" checksum="CE79778127E3275B" modified="2006-05-30" version="1">MSVVALKPYKFPARDARENFPAPLLFIGWEDHLLFAAPVALPLPSDTLFGALCTQVLPGTYGYHPDFSKIDWSQVQWFKSGQPWHPDPAKSLAENGLTHKDVIRFRTPGLNGLSGSCN</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LPG0</accession>
    <name>Q1LPG0_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Serine O-acetyltransferase</fullName>
            <ecNumber evidence="3">2.3.1.30</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">cysE</name>
        <name evidence="6" type="ordered locus">Rmet_1080</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>acetyl-CoA + L-serine = CoA + O-acetyl-L-serine</text>
            <dbReference type="Rhea" id="RHEA:24560"/>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:58340"/>
            <dbReference type="EC" id="2.3.1.30"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the transferase hexapeptide repeat family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.30" evidence="3"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07966.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515868.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1080"/>
    <dbReference type="EnsemblBacteria" id="ABF07966">
        <property type="protein sequence ID" value="ABF07966"/>
        <property type="gene ID" value="Rmet_1080"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1080"/>
    <dbReference type="eggNOG" id="COG1045">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_051638_10_0_4"/>
    <dbReference type="OMA" id="GDDVMLY"/>
    <dbReference type="OrthoDB" id="1639008at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009001">
        <property type="term" value="F:serine O-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006535">
        <property type="term" value="P:cysteine biosynthetic process from serine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03354">
        <property type="entry name" value="LbH_SAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3130.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001451">
        <property type="entry name" value="Hexapep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045304">
        <property type="entry name" value="LbH_SAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042122">
        <property type="entry name" value="Ser_AcTrfase_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005881">
        <property type="entry name" value="Ser_O-AcTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011004">
        <property type="entry name" value="Trimer_LpxA-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00132">
        <property type="entry name" value="Hexapep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51161">
        <property type="entry name" value="SSF51161"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01172">
        <property type="entry name" value="cysE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABF07966.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="247" mass="26608" checksum="E0314682137FEE5B" modified="2006-05-30" version="1">MFTRLKEDIDTIMLRDPAARSRLEVLTCYPGLHAVVFHRVANACWRGGFHWLGRWISHWSRFLTGIEIHPAVKLGRRVFIDHGMGVVIGETAEIGDDCTIYQGVTLGGTSLYKGQKRHPTLGVGVVVSAGAKVLGGFEVGAGARVGSNAVVLKPVPAGATAVGIPARIIMPDAPSTQQGAKQEFSAYGITPNADDPVSLALKSLIDNAAKQHDRIEAVLAALDRLGEHLENTPNDRFDASELRKLMK</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LPG3</accession>
    <name>Q1LPG3_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Rmet_1077</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the HicA mRNA interferase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07963.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515865.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1077"/>
    <dbReference type="EnsemblBacteria" id="ABF07963">
        <property type="protein sequence ID" value="ABF07963"/>
        <property type="gene ID" value="Rmet_1077"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1077"/>
    <dbReference type="eggNOG" id="COG1724">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_164851_4_2_4"/>
    <dbReference type="OMA" id="MDSRTLI"/>
    <dbReference type="OrthoDB" id="2053618at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.920.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012933">
        <property type="entry name" value="HicA_mRNA_interferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038570">
        <property type="entry name" value="HicA_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07927">
        <property type="entry name" value="HicA_toxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0540">Nuclease</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-1277">Toxin-antitoxin system</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006620"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABF07963.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="59" mass="6790" checksum="8A22E904CA9657C3" modified="2006-05-30" version="1">MHSSTLIRLLEQKGWKLVRTHGSHFTFKHPRHMLIVTVPHPKKDLPTGTVRNILRTAGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LPN1</accession>
    <name>Q1LPN1_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Putative NADH dehydrogenase/NAD(P)H nitroreductase Rmet_1009</fullName>
            <ecNumber evidence="5">1.-.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">rutE</name>
        <name evidence="7" type="ordered locus">Rmet_1009</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the nitroreductase family. HadB/RutE subfamily.</text>
    </comment>
    <dbReference type="EC" id="1.-.-.-" evidence="5"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07895.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008649321.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1009"/>
    <dbReference type="EnsemblBacteria" id="ABF07895">
        <property type="protein sequence ID" value="ABF07895"/>
        <property type="gene ID" value="Rmet_1009"/>
    </dbReference>
    <dbReference type="GeneID" id="60821870"/>
    <dbReference type="KEGG" id="rme:Rmet_1009"/>
    <dbReference type="eggNOG" id="COG0778">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_084441_0_0_4"/>
    <dbReference type="OMA" id="LMVVNIG"/>
    <dbReference type="OrthoDB" id="1847197at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02148">
        <property type="entry name" value="RutE-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.109.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01204">
        <property type="entry name" value="Oxidoreductase_RutE_HadB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029479">
        <property type="entry name" value="Nitroreductase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000415">
        <property type="entry name" value="Nitroreductase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023936">
        <property type="entry name" value="RutE-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00881">
        <property type="entry name" value="Nitroreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55469">
        <property type="entry name" value="SSF55469"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 5" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="2 5" id="KW-0288">FMN</keyword>
    <keyword evidence="5" id="KW-0520">NAD</keyword>
    <keyword evidence="3 5" id="KW-0521">NADP</keyword>
    <keyword evidence="4 5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nitroreductase" evidence="6">
        <location>
            <begin position="17"/>
            <end position="173"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABF07895.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="195" mass="21380" checksum="AD96C621CBCB29D2" modified="2006-05-30" version="1">MKSVDQAVLAQLFTEARTHNVWQDKHVDDALLKQIYEAMKFGPTAANGSPARIVFVKSAAEKARLVECVSAGNADKTRSAPVTAIIAFDRNFHDQLPKLFPHADARSWYAGNDEKIARDALMNSSLQGGYFILAARALGLDCGPMAGFDADKLNATFFPDGKWAVNFLVNLGYGEADKLFPRGPRLTFDEACRIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="62" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LPU7</accession>
    <name>Q1LPU7_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_0943</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07829.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515755.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0943"/>
    <dbReference type="EnsemblBacteria" id="ABF07829">
        <property type="protein sequence ID" value="ABF07829"/>
        <property type="gene ID" value="Rmet_0943"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0943"/>
    <dbReference type="eggNOG" id="COG5319">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_112041_1_0_4"/>
    <dbReference type="OMA" id="ARRMHYG"/>
    <dbReference type="OrthoDB" id="1914830at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009562">
        <property type="entry name" value="DUF1178"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06676">
        <property type="entry name" value="DUF1178"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF032131">
        <property type="entry name" value="UCP032131"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="52"/>
            <end position="73"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF07829.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="155" mass="17169" checksum="45BED0327C558ECD" modified="2006-05-30" version="1">MKVLDLRCAQDHRFEGWFASEEDAQSQISRDLVQCPVCGDHAVSRLPSAPRLNLSGATAKPAQTQVRNAEEGKADAQQMMQALQARYLKAVRQVLAQTEDVGERFAEEARRMHYEEAPERGIRGSATPEDVQALADEGIETFPLIVPDALKQTAH</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="62" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LPY2</accession>
    <name>Q1LPY2_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_0908</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07794.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515734.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0908"/>
    <dbReference type="EnsemblBacteria" id="ABF07794">
        <property type="protein sequence ID" value="ABF07794"/>
        <property type="gene ID" value="Rmet_0908"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0908"/>
    <dbReference type="eggNOG" id="ENOG5032YRY">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_085707_0_0_4"/>
    <dbReference type="OMA" id="ARENYQV"/>
    <dbReference type="OrthoDB" id="1980311at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021455">
        <property type="entry name" value="DUF3106"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11304">
        <property type="entry name" value="DUF3106"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="159"/>
            <end position="268"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF07794.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="268" mass="28621" checksum="277AA43F3ABEC760" modified="2006-05-30" version="1">MARARHLADAFHRRVARLWAAGPAVAALVAALCGGFATSAVQAQNPAASQPAAPHPITNAHPTWLELSPAHQQALAPLQPIWETLPELNRHKWERIAERYPKLKPEEQKRLQERMTEWVKLTPQQRRLARENYQITRTLPPETKAEAWDRYQQLPEEQKKKLAAAEKVPRRPGTVSALPSGKRPPADTSRQLRPEKKAASGAAAVAGPASAAQATPAPSSASAAAVAAASAPTAPAATTPAPATPPALSPPVTGEAATTFDTTDTPRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="51" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LQ28</accession>
    <name>Q1LQ28_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_0862</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07748.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515689.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0862"/>
    <dbReference type="EnsemblBacteria" id="ABF07748">
        <property type="protein sequence ID" value="ABF07748"/>
        <property type="gene ID" value="Rmet_0862"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0862"/>
    <dbReference type="HOGENOM" id="CLU_2495631_0_0_4"/>
    <dbReference type="OMA" id="MPEPKHD"/>
    <dbReference type="OrthoDB" id="1996157at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF07748.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="86" mass="9541" checksum="73EB8C07DC5C462A" modified="2006-05-30" version="1">MPEPKHDEALVNNYLERISALSVSAFDGADVGQELTQLMREAKRHCSGGNLDVLTSRLRDRIDAADREDQPQVRDTFAMAVSLIQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LQ89</accession>
    <name>Q1LQ89_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Putative glycine betaine/carnitine/choline ABC transporter, permease component: QAT family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Rmet_0801</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cell membrane</location>
            <topology evidence="4">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the binding-protein-dependent transport system permease family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07687.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008645203.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0801"/>
    <dbReference type="EnsemblBacteria" id="ABF07687">
        <property type="protein sequence ID" value="ABF07687"/>
        <property type="gene ID" value="Rmet_0801"/>
    </dbReference>
    <dbReference type="GeneID" id="60825287"/>
    <dbReference type="KEGG" id="rme:Rmet_0801"/>
    <dbReference type="eggNOG" id="COG1174">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046113_7_2_4"/>
    <dbReference type="OMA" id="NMGQATP"/>
    <dbReference type="OrthoDB" id="1233189at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="SSF161098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2 4" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="22"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="54"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="82"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="156"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="184"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="5">
        <location>
            <begin position="18"/>
            <end position="202"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABF07687.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="216" mass="22621" checksum="8E846606CA6DFDA6" modified="2006-05-30" version="1">MDLLTYLHHSWPTLLRLTGEHLALVGSAVGLAIVIGVPLGILITRHRFLAGPMLALATVVLTLPSIALFGLMIPIFARFGHALGYLPAVTAVFLYSLLPIMRNTYTALANIDPGIQEAGRGIGMTTWQRMRLVDLPLAVPVILGGVRTAVVMNIGVATIAAVIGAGGLGVLILQAISQSNMSKLAVGAVLVSLLAIVADACLQALQRALTPKGIRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="57" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LQP5</accession>
    <name>Q1LQP5_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Tad domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_0645</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07531.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0645"/>
    <dbReference type="EnsemblBacteria" id="ABF07531">
        <property type="protein sequence ID" value="ABF07531"/>
        <property type="gene ID" value="Rmet_0645"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0645"/>
    <dbReference type="eggNOG" id="COG4961">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_655079_0_0_4"/>
    <dbReference type="OMA" id="GSPCATQ"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028087">
        <property type="entry name" value="Tad_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13400">
        <property type="entry name" value="Tad"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="21"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="domain" description="Tad" evidence="2">
        <location>
            <begin position="20"/>
            <end position="67"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13400"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF07531.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="434" mass="44908" checksum="E0729FDD080DD7DB" modified="2006-05-30" version="1">MSTTRSRGMSLPRIHERQRGAVAIIVGLMIVVLVGFIGLALDLGKLYVSKSELQNRADSCALAAARDLTGATPLTVSEAAGLTAAARNLVLFQGNLEQQPNITSAESVTYSDSLANPFLDKNSVTYALNTIKYVKCDVSRGNIANWFAQVLNAIPGIHIGANTVGAFAVATTTSAQTTCAIPVYICRPDTATPAVPGGYTIGQWLTAKIGTSGGGVYGGGNFGWADLSGGKGNTPALDTQLQGAGQCNLPALNTQIGTTGNKASLSDAWNSRFGITTNKTTGVTDFTGFAYTSTTWTAQNNAYNGTSTDSKGASQPNFLAARKTYQPYQSDSVTGLSTKGSAASQATYQAGADRRLVLAPIVDCSTFSTPGTHQANVTGWACVLMVDPMQQGGNIDTVHLEYRGDSSLPGSPCATQGMPGASTGVGPLVPVLVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="104" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LR39</accession>
    <name>Q1LR39_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 7 10">Phosphoglycerate kinase</fullName>
            <ecNumber evidence="2 7 10">2.7.2.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">pgk2</name>
        <name evidence="7" type="synonym">pgk</name>
        <name evidence="11" type="ordered locus">Rmet_0501</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 7 10">
            <text>(2R)-3-phosphoglycerate + ATP = (2R)-3-phospho-glyceroyl phosphate + ADP</text>
            <dbReference type="Rhea" id="RHEA:14801"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57604"/>
            <dbReference type="ChEBI" id="CHEBI:58272"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.2.3"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="7">Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 2/5.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7 10">Belongs to the phosphoglycerate kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.7.2.3" evidence="2 7 10"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07387.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0501"/>
    <dbReference type="EnsemblBacteria" id="ABF07387">
        <property type="protein sequence ID" value="ABF07387"/>
        <property type="gene ID" value="Rmet_0501"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0501"/>
    <dbReference type="eggNOG" id="COG0126">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025427_0_2_4"/>
    <dbReference type="OMA" id="DMIFDIG"/>
    <dbReference type="UniPathway" id="UPA00109">
        <property type="reaction ID" value="UER00185"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004618">
        <property type="term" value="F:phosphoglycerate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006096">
        <property type="term" value="P:glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1260">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00145">
        <property type="entry name" value="Phosphoglyc_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001576">
        <property type="entry name" value="Phosphoglycerate_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015911">
        <property type="entry name" value="Phosphoglycerate_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015824">
        <property type="entry name" value="Phosphoglycerate_kinase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036043">
        <property type="entry name" value="Phosphoglycerate_kinase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11406">
        <property type="entry name" value="PTHR11406"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00162">
        <property type="entry name" value="PGK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000724">
        <property type="entry name" value="Pgk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00477">
        <property type="entry name" value="PHGLYCKINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53748">
        <property type="entry name" value="SSF53748"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00111">
        <property type="entry name" value="PGLYCERATE_KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="5 7 10" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7 10" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Substrate binding" evidence="7 8">
        <location>
            <begin position="46"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="7 8">
        <location>
            <begin position="85"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7 8">
        <location>
            <position position="62"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7 8">
        <location>
            <position position="139"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7 8">
        <location>
            <position position="172"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7 9">
        <location>
            <position position="223"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7 9">
        <location>
            <position position="345"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7 9">
        <location>
            <begin position="371"/>
            <end position="374"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR000724-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR000724-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ABF07387.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="418" mass="43337" checksum="F2340CB79FE6C21B" modified="2006-05-30" version="1">MPVFPISTGGACRPRPALIAMTSVLRLTDLISQGKISGKRVFIRADLNVPQDDAGNITEDTRIRASVPAIQACLEAGATVMVTSHLGRPTEGEFKPEDSLAPIATRLSELLGKPVKLVQNWVDGVEVAPGQIVLLENCRVNKGEKKNSDELAQKMAKLCDVYVNDAFGTAHRAEATTHGIAKFAPIACAGPLLAAEIDALGRALGQPARPLVAIVAGSKVSTKLTILKALADKVDNLIVGGGIANTFMLAAGLKIGKSLAEADLVGDAKTIIDIMAARGASVPIPVDVVCAKEFSATAAATVKDVKDVTDDDMILDIGPKTAAMLADQLKAAGTIVWNGPVGVFEFDQFGNGTKVLAEAIATSKAFSIAGGGDTLAAIAKYGIADRVGYISTGGGAFLEFLEGKKLPAFEVLEQRAAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LRB2</accession>
    <name>Q1LRB2_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Putative Fis-like DNA-binding protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Rmet_0428</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the transcriptional regulatory Fis family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07314.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008646031.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0428"/>
    <dbReference type="EnsemblBacteria" id="ABF07314">
        <property type="protein sequence ID" value="ABF07314"/>
        <property type="gene ID" value="Rmet_0428"/>
    </dbReference>
    <dbReference type="GeneID" id="60824030"/>
    <dbReference type="KEGG" id="rme:Rmet_0428"/>
    <dbReference type="eggNOG" id="COG2901">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_158040_2_1_4"/>
    <dbReference type="OMA" id="MVLCEVE"/>
    <dbReference type="OrthoDB" id="1933378at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005412">
        <property type="entry name" value="Fis_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002197">
        <property type="entry name" value="HTH_Fis"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02954">
        <property type="entry name" value="HTH_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002097">
        <property type="entry name" value="DNA-binding_Fis"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01590">
        <property type="entry name" value="HTHFIS"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH_8" evidence="3">
        <location>
            <begin position="34"/>
            <end position="73"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00029540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF02954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF07314.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="77" mass="8839" checksum="80A2494DC1A4B18D" modified="2006-05-30" version="1">MSRNAIDQCIRDSLDTYFRDLDGEVPSNMYNMVLEAVERPLLEAVMTRAERNQSLAAAYLGINRNTLRKKLQQHGLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LRH9</accession>
    <name>Q1LRH9_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Carbon monoxide dehydrogenase accessory protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">coxE</name>
        <name evidence="3" type="ordered locus">Rmet_0361</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07247.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0361"/>
    <dbReference type="EnsemblBacteria" id="ABF07247">
        <property type="protein sequence ID" value="ABF07247"/>
        <property type="gene ID" value="Rmet_0361"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0361"/>
    <dbReference type="eggNOG" id="COG3552">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_042261_0_1_4"/>
    <dbReference type="OMA" id="DFYWTLH"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.410">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011195">
        <property type="entry name" value="UCP010256"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008912">
        <property type="entry name" value="Uncharacterised_CoxE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002035">
        <property type="entry name" value="VWF_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036465">
        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05762">
        <property type="entry name" value="VWA_CoxE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF010256">
        <property type="entry name" value="CoxE_vWa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00327">
        <property type="entry name" value="VWA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53300">
        <property type="entry name" value="SSF53300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="VWFA" evidence="2">
        <location>
            <begin position="235"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="133"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00327"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF07247.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="428" mass="48391" checksum="69A3DB5441217D9D" modified="2006-05-30" version="1">MPRAVPSAPSAPFAPFSRPDTSGTPPPLPMLARNVTHFMRLLRDTGFGLSPAHAVDALEALRHIDIGARDEVRAALAALVLSGPDQRPLFDAAFDLFWRDPDWESKLRAMLLPRVDAGIPPPRRSNRLADALAARQPPSPKPDQPPREERFTAPLTFSDQERLAGRDFETLNAEEWRALQHLVRTRRARLALQKTRRLRPSTCGTHADLRASARLAVRQHGEWLRWKYRKRAERKPPVVLLLDISGSMSQYSRAVLYFCHALTQSRERLHVFLFGTRLTNVTRALRERDPDDAVNLITEQVRDWAGGTRIGAALATFNRQWARRTLSGRATVLLVSDGIDLEHIDLLEQEMARLRRFAHRIIWLNPLLRYEGFTPQARGIQAILPHVDALRAAHNLDSLFALESLLADPARVPPVKASRPQPGYPSWK</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LRI1</accession>
    <name>Q1LRI1_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Carbon monoxide dehydrogenase accessory protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">coxI</name>
        <name evidence="3" type="ordered locus">Rmet_0359</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07245.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0359"/>
    <dbReference type="EnsemblBacteria" id="ABF07245">
        <property type="protein sequence ID" value="ABF07245"/>
        <property type="gene ID" value="Rmet_0359"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0359"/>
    <dbReference type="eggNOG" id="COG1975">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041115_2_0_4"/>
    <dbReference type="OMA" id="HTPIHHD"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003777">
        <property type="entry name" value="XdhC_CoxI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027051">
        <property type="entry name" value="XdhC_Rossmann_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13478">
        <property type="entry name" value="XdhC_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02625">
        <property type="entry name" value="XdhC_CoxI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="XdhC_CoxI" evidence="1">
        <location>
            <begin position="12"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="domain" description="XdhC_C" evidence="2">
        <location>
            <begin position="162"/>
            <end position="303"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02625"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF07245.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="345" mass="36922" checksum="1C20EA67AA6A3C55" modified="2006-05-30" version="1">MDLEVLKCSVRWQQEGHGVLLVTVVRTWGSSPRPEGAMLAVRDDGHVVGSVSGGCIEDDIIDRVRHEGIHSGLPEAIKYGISAEEAHRFGLPCGGTIELVTEPLSAASGIVDLLDAVENGRLIARTLDMATGAATLGPAEATDGLAFDGKTLLTIHGPRYRMLVIGAGQLSKYLCQIAVGLGFQVTVCDPREEYTETWDIAGVTMVRTMPDDTVMEMKLDERCAVIALTHDPKLDDLALMEALRTPAFYVGALGSRRNNHARRERLKEFDLTELQLARLHGPVGIYIGSRTPPEIAISILAEVVAAKNHVSLPDILQVEGAKAAREIAAGEAACPILPTASAAQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="69" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LRK6</accession>
    <name>Q1LRK6_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">S-adenosyl-L-methionine-dependent methyltransferase</fullName>
            <ecNumber evidence="2">2.1.1.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">arsM</name>
        <name evidence="2" type="ordered locus">Rmet_0334</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.1.1.-" evidence="2"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07220.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515215.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0334"/>
    <dbReference type="EnsemblBacteria" id="ABF07220">
        <property type="protein sequence ID" value="ABF07220"/>
        <property type="gene ID" value="Rmet_0334"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0334"/>
    <dbReference type="eggNOG" id="COG2226">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1041027_0_0_4"/>
    <dbReference type="OMA" id="RENIWQL"/>
    <dbReference type="OrthoDB" id="1431378at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041698">
        <property type="entry name" value="Methyltransf_25"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13649">
        <property type="entry name" value="Methyltransf_25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Methyltransf_25" evidence="1">
        <location>
            <begin position="51"/>
            <end position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF07220.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="272" mass="30597" checksum="DF9AFBB959063D79" modified="2006-05-30" version="1">MSTAVAQDTINRKAWRSWGARRWFKILSDWTDPGEAAALAFVADAVRDTPILDVGVGGGRTVPMLRKLSQHYEAIDYMPEMVEICQHNHPDIRVSQMDARDLSAFEDNTFGLVVFSFNGIDAVDYAGRRAVLAEFARVLRPGGMLVFSTHNLHGPTYRENLTQFLRLPAWSNNPIRLGFNVARTVVNLPLATINYLRNSQLNREFDGYAVRVCAAHKFGIVIVYTDVPTQLRELKACGLQTEAVFGNLNDKAFQPGDPLDDVNWFHFVARKL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-02-26" modified="2022-05-25" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LRM9</accession>
    <name>APT_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Adenine phosphoribosyltransferase</fullName>
            <shortName evidence="1">APRT</shortName>
            <ecNumber evidence="1">2.4.2.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">apt</name>
        <name type="ordered locus">Rmet_0311</name>
    </gene>
    <organism>
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes a salvage reaction resulting in the formation of AMP, that is energically less costly than de novo synthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>AMP + diphosphate = 5-phospho-alpha-D-ribose 1-diphosphate + adenine</text>
            <dbReference type="Rhea" id="RHEA:16609"/>
            <dbReference type="ChEBI" id="CHEBI:16708"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="2.4.2.7"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Purine metabolism; AMP biosynthesis via salvage pathway; AMP from adenine: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the purine/pyrimidine phosphoribosyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.7" evidence="1"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07197.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515197.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q1LRM9"/>
    <dbReference type="SMR" id="Q1LRM9"/>
    <dbReference type="STRING" id="266264.Rmet_0311"/>
    <dbReference type="EnsemblBacteria" id="ABF07197">
        <property type="protein sequence ID" value="ABF07197"/>
        <property type="gene ID" value="Rmet_0311"/>
    </dbReference>
    <dbReference type="GeneID" id="60823925"/>
    <dbReference type="KEGG" id="rme:Rmet_0311"/>
    <dbReference type="eggNOG" id="COG0503">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_063339_3_0_4"/>
    <dbReference type="OMA" id="KPGIVFR"/>
    <dbReference type="OrthoDB" id="1532478at2"/>
    <dbReference type="UniPathway" id="UPA00588">
        <property type="reaction ID" value="UER00646"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003999">
        <property type="term" value="F:adenine phosphoribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006168">
        <property type="term" value="P:adenine salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044209">
        <property type="term" value="P:AMP salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006166">
        <property type="term" value="P:purine ribonucleoside salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06223">
        <property type="entry name" value="PRTases_typeI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2020">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00004">
        <property type="entry name" value="Aden_phosphoribosyltr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005764">
        <property type="entry name" value="Ade_phspho_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000836">
        <property type="entry name" value="PRibTrfase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029057">
        <property type="entry name" value="PRTase-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00156">
        <property type="entry name" value="Pribosyltran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53271">
        <property type="entry name" value="SSF53271"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01090">
        <property type="entry name" value="apt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00103">
        <property type="entry name" value="PUR_PYR_PR_TRANSFER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0328">Glycosyltransferase</keyword>
    <keyword id="KW-0660">Purine salvage</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000321390" description="Adenine phosphoribosyltransferase">
        <location>
            <begin position="1"/>
            <end position="190"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00004"/>
        </source>
    </evidence>
    <sequence length="190" mass="20740" checksum="003A75CC0F8465CE" modified="2006-05-30" version="1">MSNSIIQSPDLGDVTGYLRDRIRTVPDWPQPGVMFRDITPLLQNPKTLRVLIDVFVHRYMDQNLDLVAGIDARGFILGSIIAYELNLGFVPIRKKGKLPFQTVAEEYELEYGSATVEIHADACKPGDRVLLIDDLIATGGTMMAGRKLLERLGATVVEGGAIVDLPELGGSKLLQASGLSLFTVCNFDGH</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="104" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LRN2</accession>
    <name>Q1LRN2_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Probable glutathione-regulated potassium-efflux system k+/h+ antiporter transmembrane protein Kef-type K+ transport system</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">Rmet_0308</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07194.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0308"/>
    <dbReference type="EnsemblBacteria" id="ABF07194">
        <property type="protein sequence ID" value="ABF07194"/>
        <property type="gene ID" value="Rmet_0308"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0308"/>
    <dbReference type="eggNOG" id="COG0475">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG0490">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG1226">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005126_9_0_4"/>
    <dbReference type="OMA" id="IRPFHDV"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008324">
        <property type="term" value="F:cation transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015299">
        <property type="term" value="F:solute:proton antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006813">
        <property type="term" value="P:potassium ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1530.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1450">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006153">
        <property type="entry name" value="Cation/H_exchanger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038770">
        <property type="entry name" value="Na+/solute_symporter_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006037">
        <property type="entry name" value="RCK_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036721">
        <property type="entry name" value="RCK_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003148">
        <property type="entry name" value="RCK_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00999">
        <property type="entry name" value="Na_H_Exchanger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02080">
        <property type="entry name" value="TrkA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02254">
        <property type="entry name" value="TrkA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF116726">
        <property type="entry name" value="SSF116726"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51202">
        <property type="entry name" value="RCK_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51201">
        <property type="entry name" value="RCK_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0406">Ion transport</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0630">Potassium</keyword>
    <keyword evidence="1" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="20"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="46"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="70"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="100"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="128"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="169"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="194"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="235"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="288"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="313"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="341"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="372"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="domain" description="RCK N-terminal" evidence="7">
        <location>
            <begin position="427"/>
            <end position="549"/>
        </location>
    </feature>
    <feature type="domain" description="RCK C-terminal" evidence="8">
        <location>
            <begin position="589"/>
            <end position="673"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ABF07194.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="674" mass="73145" checksum="88908BA8A744814D" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-04-08" modified="2022-05-25" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LRS0</accession>
    <name>COXX_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Protoheme IX farnesyltransferase</fullName>
            <ecNumber evidence="1">2.5.1.141</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Heme B farnesyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Heme O synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">ctaB</name>
        <name type="ordered locus">Rmet_0270</name>
    </gene>
    <organism>
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Converts heme B (protoheme IX) to heme O by substitution of the vinyl group on carbon 2 of heme B porphyrin ring with a hydroxyethyl farnesyl side group.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(2E,6E)-farnesyl diphosphate + H2O + heme b = diphosphate + Fe(II)-heme o</text>
            <dbReference type="Rhea" id="RHEA:28070"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
            <dbReference type="ChEBI" id="CHEBI:60530"/>
            <dbReference type="ChEBI" id="CHEBI:175763"/>
            <dbReference type="EC" id="2.5.1.141"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Porphyrin-containing compound metabolism; heme O biosynthesis; heme O from protoheme: step 1/1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="miscellaneous">
        <text evidence="1">Carbon 2 of the heme B porphyrin ring is defined according to the Fischer nomenclature.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the UbiA prenyltransferase family. Protoheme IX farnesyltransferase subfamily.</text>
    </comment>
    <comment type="sequence caution" evidence="2">
        <conflict type="erroneous initiation">
            <sequence resource="EMBL-CDS" id="ABF07156" version="1"/>
        </conflict>
    </comment>
    <dbReference type="EC" id="2.5.1.141" evidence="1"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07156.1"/>
        <property type="status" value="ALT_INIT"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q1LRS0"/>
    <dbReference type="SMR" id="Q1LRS0"/>
    <dbReference type="STRING" id="266264.Rmet_0270"/>
    <dbReference type="EnsemblBacteria" id="ABF07156">
        <property type="protein sequence ID" value="ABF07156"/>
        <property type="gene ID" value="Rmet_0270"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0270"/>
    <dbReference type="eggNOG" id="COG0109">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029631_0_2_4"/>
    <dbReference type="UniPathway" id="UPA00834">
        <property type="reaction ID" value="UER00712"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008495">
        <property type="term" value="F:protoheme IX farnesyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048034">
        <property type="term" value="P:heme O biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd13957">
        <property type="entry name" value="PT_UbiA_Cox10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.357.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00154">
        <property type="entry name" value="CyoE_CtaB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006369">
        <property type="entry name" value="Protohaem_IX_farnesylTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000537">
        <property type="entry name" value="UbiA_prenyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030470">
        <property type="entry name" value="UbiA_prenylTrfase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044878">
        <property type="entry name" value="UbiA_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43448">
        <property type="entry name" value="PTHR43448"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01040">
        <property type="entry name" value="UbiA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01473">
        <property type="entry name" value="cyoE_ctaB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00943">
        <property type="entry name" value="UBIA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0997">Cell inner membrane</keyword>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0350">Heme biosynthesis</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000327127" description="Protoheme IX farnesyltransferase">
        <location>
            <begin position="1"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="29"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="51"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="101"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="123"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="151"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="177"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="221"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="244"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="283"/>
            <end position="303"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="305" mass="33576" checksum="B6B5EBC28D1C0DD6" modified="2008-04-08" version="2">MVTATHSQSSVGRLRHLARQYAALTKPRVTQLAVFCAIIGMFLATPGMVPWSVLIGGAAGIWLLAGAAFAINCLVEQKIDALMRRTAWRPSATGEITTTQTLIFSAVLGGAGMWLLHVFANDLTMWLTFATFLGYAVVYTILLKPATPQNIVIGGLSGAMPPALGWSAVSGTVPAEAWFLVLIIFTWTPPHFWALALYRRADYAKSGLPMLPITHGERYTLLHILLYTLIMIAATLLPFVYGMSGYIYLVAALGLGLVFLGYAWKLYRNYSDGLAQRTFRYSILYLSLLFAVLLVDHYFKFVPQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LRT8</accession>
    <name>Q1LRT8_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Glutaredoxin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">grxC</name>
        <name evidence="5" type="ordered locus">Rmet_0252</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Has a glutathione-disulfide oxidoreductase activity in the presence of NADPH and glutathione reductase. Reduces low molecular weight disulfides and proteins.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the glutaredoxin family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07138.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515148.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0252"/>
    <dbReference type="EnsemblBacteria" id="ABF07138">
        <property type="protein sequence ID" value="ABF07138"/>
        <property type="gene ID" value="Rmet_0252"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0252"/>
    <dbReference type="eggNOG" id="COG0695">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026126_7_3_4"/>
    <dbReference type="OMA" id="LLWWKGV"/>
    <dbReference type="OrthoDB" id="2045232at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097573">
        <property type="term" value="F:glutathione oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004362">
        <property type="term" value="F:glutathione-disulfide reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045454">
        <property type="term" value="P:cell redox homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03418">
        <property type="entry name" value="GRX_GRXb_1_3_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011767">
        <property type="entry name" value="GLR_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002109">
        <property type="entry name" value="Glutaredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014025">
        <property type="entry name" value="Glutaredoxin_subgr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011900">
        <property type="entry name" value="GRX_bact"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00462">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00160">
        <property type="entry name" value="GLUTAREDOXIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="SSF52833"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02181">
        <property type="entry name" value="GRX_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00195">
        <property type="entry name" value="GLUTAREDOXIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0249">Electron transport</keyword>
    <keyword evidence="2 3" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Glutaredoxin" evidence="4">
        <location>
            <begin position="4"/>
            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU364065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABF07138.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="85" mass="9594" checksum="10BB2C92BFC6DCF5" modified="2006-05-30" version="1">MARVVMYSTVVCPYCQMAERLLKQRGVEAIEKILIDREPGKREEMMTRTGRRTVPQIYIDETHVGGFDDLSALDRQGGLVPLLAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LS81</accession>
    <name>Q1LS81_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">2-hydroxychromene-2-carboxylate isomerase</fullName>
            <ecNumber evidence="1">5.99.1.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Rmet_0109</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>2-hydroxychromene-2-carboxylate = (3E)-4-(2-hydroxyphenyl)-2-oxobut-3-enoate</text>
            <dbReference type="Rhea" id="RHEA:27401"/>
            <dbReference type="ChEBI" id="CHEBI:59350"/>
            <dbReference type="ChEBI" id="CHEBI:59353"/>
            <dbReference type="EC" id="5.99.1.4"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the GST superfamily. NadH family.</text>
    </comment>
    <dbReference type="EC" id="5.99.1.4" evidence="1"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF06995.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515029.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0109"/>
    <dbReference type="EnsemblBacteria" id="ABF06995">
        <property type="protein sequence ID" value="ABF06995"/>
        <property type="gene ID" value="Rmet_0109"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0109"/>
    <dbReference type="eggNOG" id="COG3917">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069253_1_3_4"/>
    <dbReference type="OMA" id="AFHNIQP"/>
    <dbReference type="OrthoDB" id="996965at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018845">
        <property type="term" value="F:2-hydroxychromene-2-carboxylate isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901170">
        <property type="term" value="P:naphthalene catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03022">
        <property type="entry name" value="DsbA_HCCA_Iso"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001853">
        <property type="entry name" value="DSBA-like_thioredoxin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014440">
        <property type="entry name" value="HCCAis_GSTk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044087">
        <property type="entry name" value="NahD-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01323">
        <property type="entry name" value="DSBA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006386">
        <property type="entry name" value="HCCAis_GSTk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="SSF52833"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 4" id="KW-0413">Isomerase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DSBA" evidence="3">
        <location>
            <begin position="5"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="2">
        <location>
            <position position="13"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRNR" id="PIRNR006386"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR006386-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF06995.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="219" mass="24755" checksum="F04B0EF6C1EF74E3" modified="2006-05-30" version="1">MTAPIDFYFDFSSPYGYFASTRIDDIAQKYGRYVIWHPILLGVVFKTTGSSPLPSIPLKGDYCWRDFERTSEFNHIDYKRPTHFPLPTRHAARAMLWLQNNHGADVATAFAKAVYRALFVDDINIAEPAELAKLAEPLGVDPIAMNEGANNYQIKDQLKAEIDVAMAKGVFGSPFVIVDGEPFWGFDRFDQVEAYLKSRRQTELRAVGGNELNKEKKPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="101" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LSA0</accession>
    <name>Q1LSA0_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Response regulator, NarL-family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Rmet_0090</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF06976.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515011.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0090"/>
    <dbReference type="EnsemblBacteria" id="ABF06976">
        <property type="protein sequence ID" value="ABF06976"/>
        <property type="gene ID" value="Rmet_0090"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0090"/>
    <dbReference type="eggNOG" id="COG2197">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_90_1_4"/>
    <dbReference type="OMA" id="NVLRKTQ"/>
    <dbReference type="OrthoDB" id="1687028at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06170">
        <property type="entry name" value="LuxR_C_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016032">
        <property type="entry name" value="Sig_transdc_resp-reg_C-effctor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000792">
        <property type="entry name" value="Tscrpt_reg_LuxR_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00196">
        <property type="entry name" value="GerE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00038">
        <property type="entry name" value="HTHLUXR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00421">
        <property type="entry name" value="HTH_LUXR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46894">
        <property type="entry name" value="SSF46894"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00622">
        <property type="entry name" value="HTH_LUXR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50043">
        <property type="entry name" value="HTH_LUXR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Response regulatory" evidence="4">
        <location>
            <begin position="3"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="domain" description="HTH luxR-type" evidence="3">
        <location>
            <begin position="140"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="2">
        <location>
            <position position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABF06976.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="210" mass="23336" checksum="4B8304114E5FB73E" modified="2006-05-30" version="1">MIRVLIADDHEIVRAGLRQFIAEEPDIEVTGEAGSGDEVMAQLRDSEFDVLVLDISMPDRNGIDVLKLIRQRLPALPVLILSTYPEDQYAINLIRAGASGYLTKESAPDDLVKAIRTVAQGRRYVSPTVADLLIGGLDKPTDQPVHRTLSEREFQIFCKLSRGQSVSVIADELFLSVKTVSTYRSRILEKMGMKTNADLTYYAIKNGLVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LSD7</accession>
    <name>Q1LSD7_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Rod shape-determining protein MreD</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">mreD</name>
        <name evidence="9" type="ordered locus">Rmet_0053</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MreD family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF06939.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008640831.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0053"/>
    <dbReference type="EnsemblBacteria" id="ABF06939">
        <property type="protein sequence ID" value="ABF06939"/>
        <property type="gene ID" value="Rmet_0053"/>
    </dbReference>
    <dbReference type="GeneID" id="60822772"/>
    <dbReference type="KEGG" id="rme:Rmet_0053"/>
    <dbReference type="eggNOG" id="COG2891">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_119315_1_0_4"/>
    <dbReference type="OMA" id="YWAMALP"/>
    <dbReference type="OrthoDB" id="1888959at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008360">
        <property type="term" value="P:regulation of cell shape"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007227">
        <property type="entry name" value="Cell_shape_determining_MreD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026034">
        <property type="entry name" value="MreD_proteobac"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37484">
        <property type="entry name" value="PTHR37484"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04093">
        <property type="entry name" value="MreD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF018472">
        <property type="entry name" value="MreD_proteobac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03426">
        <property type="entry name" value="shape_MreD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5" id="KW-0133">Cell shape</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="12"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="35"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="133"/>
            <end position="156"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ABF06939.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="170" mass="19090" checksum="49FA600839417261" modified="2006-05-30" version="1">MTTSQYLLRPVNPAFIALTFVLAFLINLMPWGETLWIPDVVALVLVFWNIHQPRKVGMGVAFLLGLLMDVHDARLLGEHALAYTLLAYFAITIHRRVLWFSVYAQALHVLPLLFIAHAVPVVIRLAMGAPLPGWQLLLAPVIEAVLWPMATSLLLAPQRRSNEVDETRPI</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-01-04" modified="2022-01-19" version="124" xmlns="http://uniprot.org/uniprot">
    <accession>Q5NUU7</accession>
    <name>Q5NUU7_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Mercuric resistance operon regulatory protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">merR</name>
        <name evidence="11" type="ordered locus">Rmet_6171</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="11 12">
        <name>pMOL30</name>
    </geneLocation>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="12">Plasmid pMOL30</plasmid>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Mediates the mercuric-dependent induction of mercury resistance operon. In the absence of mercury MerR represses transcription by binding tightly to the mer operator region; when mercury is present the dimeric complex binds a single ion and becomes a potent transcriptional activator, while remaining bound to the mer site.</text>
    </comment>
    <dbReference type="EMBL" id="CP000354">
        <property type="protein sequence ID" value="ABF13030.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_003131969.1">
        <property type="nucleotide sequence ID" value="NC_007972.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_145639.1">
        <property type="nucleotide sequence ID" value="NC_006466.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_161730.1">
        <property type="nucleotide sequence ID" value="NC_006525.1"/>
    </dbReference>
    <dbReference type="SMR" id="Q5NUU7"/>
    <dbReference type="EnsemblBacteria" id="ABF13030">
        <property type="protein sequence ID" value="ABF13030"/>
        <property type="gene ID" value="Rmet_6171"/>
    </dbReference>
    <dbReference type="GeneID" id="61089172"/>
    <dbReference type="KEGG" id="rme:Rmet_6171"/>
    <dbReference type="HOGENOM" id="CLU_060077_2_0_4"/>
    <dbReference type="OrthoDB" id="1748559at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid pMOL30"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045340">
        <property type="term" value="F:mercury ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046689">
        <property type="term" value="P:response to mercury ion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04783">
        <property type="entry name" value="HTH_MerR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009061">
        <property type="entry name" value="DNA-bd_dom_put_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011794">
        <property type="entry name" value="MerR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000551">
        <property type="entry name" value="MerR-type_HTH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015358">
        <property type="entry name" value="Tscrpt_reg_MerR_DNA-bd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00376">
        <property type="entry name" value="MerR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09278">
        <property type="entry name" value="MerR-DNA-bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00040">
        <property type="entry name" value="HTHMERR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00422">
        <property type="entry name" value="HTH_MERR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46955">
        <property type="entry name" value="SSF46955"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02051">
        <property type="entry name" value="MerR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00552">
        <property type="entry name" value="HTH_MERR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50937">
        <property type="entry name" value="HTH_MERR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0010">Activator</keyword>
    <keyword evidence="6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0475">Mercuric resistance</keyword>
    <keyword evidence="4" id="KW-0476">Mercury</keyword>
    <keyword evidence="11" id="KW-0614">Plasmid</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0678">Repressor</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH merR-type" evidence="10">
        <location>
            <begin position="7"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00017146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00024874"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ABF13030.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="144" mass="15763" checksum="C573298AFF0846EF" modified="2005-01-04" version="1">MENNLENLTIGVFAKAAGVNVETIRFYQRKGLLLEPDKPYGSIRRYGEADVTRVRFVKSAQRLGFSLDEIAELLRLEDGTHCEEASSLAEHKLKDVREKMADLARMEAVLSELVCACHARRGNVSCPLIASLQGGASLAGSAMP</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-03-23" modified="2020-10-07" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>D3DXK4</accession>
    <name>D3DXK4_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_6406</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ADC45024.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_6406"/>
    <dbReference type="EnsemblBacteria" id="ADC45024">
        <property type="protein sequence ID" value="ADC45024"/>
        <property type="gene ID" value="Rmet_6406"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6406"/>
    <dbReference type="HOGENOM" id="CLU_2976087_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADC45024.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="58" mass="6372" checksum="C262840A3042DC8C" modified="2010-03-23" version="1">MRAREVPHVMAGRSLQGRRSKRHHQNDLASLRGLAVLLVLSAAVRLARTASLHFDSAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-03-23" modified="2020-10-07" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>D3DXL7</accession>
    <name>D3DXL7_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_6419</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ADC45037.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_6419"/>
    <dbReference type="EnsemblBacteria" id="ADC45037">
        <property type="protein sequence ID" value="ADC45037"/>
        <property type="gene ID" value="Rmet_6419"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6419"/>
    <dbReference type="HOGENOM" id="CLU_2864646_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADC45037.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="64" mass="7218" checksum="B08880B846AA2928" modified="2010-03-23" version="1">MQSDCRTCGACRLLPERIAAIVAPASEAGAMSFLRRKILGYIPRLLQRGLKPNRKVRTINLHVW</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-03-23" modified="2020-10-07" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>D3DXN5</accession>
    <name>D3DXN5_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_6438</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ADC45055.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_6438"/>
    <dbReference type="EnsemblBacteria" id="ADC45055">
        <property type="protein sequence ID" value="ADC45055"/>
        <property type="gene ID" value="Rmet_6438"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6438"/>
    <dbReference type="HOGENOM" id="CLU_3029132_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADC45055.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="55" mass="6131" checksum="30DD51588BC623F4" modified="2010-03-23" version="1">MPMCWSWRPVWACIVAGPTMRHMPRGGAWSGSHGPICAANLTVFGEKWGQYHFGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-03-23" modified="2020-10-07" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>D3DXZ7</accession>
    <name>D3DXZ7_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_6561</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ADC45167.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADC45167">
        <property type="protein sequence ID" value="ADC45167"/>
        <property type="gene ID" value="Rmet_6561"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6561"/>
    <dbReference type="HOGENOM" id="CLU_1894493_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADC45167.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="134" mass="15174" checksum="0A0A4DC4854B8F2C" modified="2010-03-23" version="1">MHRYGVRWHSKALVIEKDFPLNFELLSACLEKVARALYFHHHRGQRKLFGNLKVCPLFIPVEPRVTPELALALSKVRAKTDLDFEQLPRLGPHQEIFAYQVIETPNIVAVNMEFYGAHRASVMGGVPAAARPSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-03-23" modified="2020-10-07" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>D3DY07</accession>
    <name>D3DY07_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_6575</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ADC45177.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_6575"/>
    <dbReference type="EnsemblBacteria" id="ADC45177">
        <property type="protein sequence ID" value="ADC45177"/>
        <property type="gene ID" value="Rmet_6575"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6575"/>
    <dbReference type="HOGENOM" id="CLU_2976090_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADC45177.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="58" mass="6215" checksum="5AEC63F1399FE6E8" modified="2010-03-23" version="1">MGARAVSGDSSAIHCLTDACANGEAAPLSTLRTHHLVYRHVTASFGKIVCLHNDIRRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-03-23" modified="2020-10-07" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>D3DY31</accession>
    <name>D3DY31_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_6600</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ADC45201.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_6600"/>
    <dbReference type="EnsemblBacteria" id="ADC45201">
        <property type="protein sequence ID" value="ADC45201"/>
        <property type="gene ID" value="Rmet_6600"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6600"/>
    <dbReference type="HOGENOM" id="CLU_2864650_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADC45201.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="64" mass="7670" checksum="6B0226A52411D7A6" modified="2010-03-23" version="1">MARWRSTKWSLRHRYAGDYCVRLLRRKTSGFALGVIHARYVTHATDVRARTDVCERRRNCVKMM</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-03-23" modified="2020-10-07" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>D3DY64</accession>
    <name>D3DY64_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_6634</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ADC45234.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_6634"/>
    <dbReference type="EnsemblBacteria" id="ADC45234">
        <property type="protein sequence ID" value="ADC45234"/>
        <property type="gene ID" value="Rmet_6634"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6634"/>
    <dbReference type="HOGENOM" id="CLU_1894495_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADC45234.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="134" mass="14776" checksum="0FE4DCC8370C4E81" modified="2010-03-23" version="1">MLIELIQRHAAQMLHCRSGVTKGSEEDLVSAQSVLGDTRPASVTVEVIASNLWDPISGLGLLLRSARRCSLRFGHELARMTVTAITEDCARQSEYGERGGHLSHPASRTRARPALCISRPAIWRKQGRMAMTVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-03-23" modified="2020-10-07" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>D3DYC0</accession>
    <name>D3DYC0_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_6710</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ADC45290.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_6710"/>
    <dbReference type="EnsemblBacteria" id="ADC45290">
        <property type="protein sequence ID" value="ADC45290"/>
        <property type="gene ID" value="Rmet_6710"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6710"/>
    <dbReference type="HOGENOM" id="CLU_2919467_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADC45290.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="61" mass="6786" checksum="FE15BEDC9D168C7E" modified="2010-03-23" version="1">MRWSWMAPGRTSRIFFTGQDTDTSRTGISPFFPFDAPVAPAYHSVTVTGQTQFRQSLEGAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-03-23" modified="2020-10-07" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>D3DYG4</accession>
    <name>D3DYG4_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_6755</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ADC45334.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_6755"/>
    <dbReference type="EnsemblBacteria" id="ADC45334">
        <property type="protein sequence ID" value="ADC45334"/>
        <property type="gene ID" value="Rmet_6755"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6755"/>
    <dbReference type="HOGENOM" id="CLU_2289296_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="81"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADC45334.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="101" mass="11337" checksum="C1F772712053A64A" modified="2010-03-23" version="1">MAKTPHSRGKNEGDGNGSHLKEQPSERLRIGLVDYGSLRGTLKTSCNVLHGRNQSGARITITYQWYFPIKFDDDAAVMAGQRGRAKTNHTKTRKRPNQQPM</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-03-23" modified="2020-10-07" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>D3DYH4</accession>
    <name>D3DYH4_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_6766</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ADC45344.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADC45344">
        <property type="protein sequence ID" value="ADC45344"/>
        <property type="gene ID" value="Rmet_6766"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6766"/>
    <dbReference type="HOGENOM" id="CLU_2719425_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="27"/>
            <end position="47"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADC45344.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="72" mass="7411" checksum="EF7F6DC19F8400B5" modified="2010-03-23" version="1">MLAISVWFLASLGVGMFATALGRSSPAWILFSLILSPSLGLIVVAVLDKKRVRSARVLATSSSPAAAISVPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-03-23" modified="2020-10-07" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>D3DYJ9</accession>
    <name>D3DYJ9_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_6401</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2 3">
        <name>pMOL28</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid pMOL28</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000355">
        <property type="protein sequence ID" value="ADC45369.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADC45369">
        <property type="protein sequence ID" value="ADC45369"/>
        <property type="gene ID" value="Rmet_6401"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6401"/>
    <dbReference type="HOGENOM" id="CLU_3232468_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid pMOL28"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="16"/>
            <end position="34"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADC45369.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="43" mass="4916" checksum="B064D4528D728868" modified="2010-03-23" version="1">MKFFDFLSTNHDINRFVFGTVICYCVVEAVVAVYRGAKARKKA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1990-01-01" modified="2022-05-25" version="128" xmlns="http://uniprot.org/uniprot">
    <accession>P13509</accession>
    <accession>P94141</accession>
    <accession>Q1LAI5</accession>
    <accession>Q44010</accession>
    <name>CZCC_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName>Cobalt-zinc-cadmium resistance protein CzcC</fullName>
        </recommendedName>
        <alternativeName>
            <fullName>Cation efflux system protein CzcC</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">czcC</name>
        <name type="ordered locus">Rmet_5982</name>
    </gene>
    <organism>
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid">
        <name>pMOL30</name>
    </geneLocation>
    <reference key="1">
        <citation type="journal article" date="1989" name="Proc. Natl. Acad. Sci. U.S.A." volume="86" first="7351" last="7355">
            <title>Expression and nucleotide sequence of a plasmid-determined divalent cation efflux system from Alcaligenes eutrophus.</title>
            <authorList>
                <person name="Nies D.H."/>
                <person name="Nies A."/>
                <person name="Chu L."/>
                <person name="Silver S."/>
            </authorList>
            <dbReference type="PubMed" id="2678100"/>
            <dbReference type="DOI" id="10.1073/pnas.86.19.7351"/>
        </citation>
        <scope>PRELIMINARY NUCLEOTIDE SEQUENCE [GENOMIC DNA]</scope>
    </reference>
    <reference key="2">
        <citation type="submission" date="2004-11" db="EMBL/GenBank/DDBJ databases">
            <title>Sequence and features of the Ralstonia metallidurans CH34 heavy metals plasmids pMOL28 and pMOL30.</title>
            <authorList>
                <person name="Monchy S."/>
                <person name="van der Lelie D."/>
                <person name="Vallaeys T."/>
                <person name="Taghavi S."/>
                <person name="Benotmane M."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="Lapidus A."/>
                <person name="Mergeay M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference key="3">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <reference key="4">
        <citation type="journal article" date="1995" name="J. Ind. Microbiol." volume="14" first="142" last="153">
            <title>The czc operon of Alcaligenes eutrophus CH34: from resistance mechanism to the removal of heavy metals.</title>
            <authorList>
                <person name="Diels L."/>
                <person name="Dong Q."/>
                <person name="van der Lelie D."/>
                <person name="Baeyens W."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="7766206"/>
            <dbReference type="DOI" id="10.1007/BF01569896"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-86</scope>
        <scope>CHARACTERIZATION</scope>
        <scope>INDUCTION</scope>
        <scope>DISCUSSION OF FUNCTION</scope>
    </reference>
    <comment type="function">
        <text>CzcC protein appears to modify the specificity of the system, perhaps by acting on the CzcB protein. When the CzcC protein is added to CzcA and CzcB, the efflux system gains specificity for cadmium and cobalt.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell outer membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="induction">
        <text evidence="2">By zinc, cadmium and cobalt (zinc being the best and cobalt being the worst inducer).</text>
    </comment>
    <comment type="biotechnology">
        <text evidence="2">In the presence of 2 mM Cd(2+) or 4-10 mM Zn(2+) up to 99% of the metal is removed from the culture supernatant and is sequestered via bioprecipitation. Additionally Cu(2+), Co(2+), Ni(2+), Pb(2+), Y(3+) and Ge(4+) can also be removed from culture supernatants, although it is not clear which efflux system is responsible for all these substrates (PubMed:7766206).</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the outer membrane factor (OMF) (TC 1.B.17) family.</text>
    </comment>
    <comment type="sequence caution" evidence="3">
        <conflict type="erroneous initiation">
            <sequence resource="EMBL-CDS" id="CAA50525" version="2"/>
        </conflict>
    </comment>
    <dbReference type="EMBL" id="X98451">
        <property type="protein sequence ID" value="CAA67082.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP000354">
        <property type="protein sequence ID" value="ABF12841.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="X71400">
        <property type="protein sequence ID" value="CAA50525.2"/>
        <property type="status" value="ALT_INIT"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011514819.1">
        <property type="nucleotide sequence ID" value="NC_007971.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_145593.1">
        <property type="nucleotide sequence ID" value="NC_006466.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="P13509"/>
    <dbReference type="SMR" id="P13509"/>
    <dbReference type="TCDB" id="1.B.17.2.2">
        <property type="family name" value="the outer membrane factor (omf) family"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF12841">
        <property type="protein sequence ID" value="ABF12841"/>
        <property type="gene ID" value="Rmet_5982"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5982"/>
    <dbReference type="HOGENOM" id="CLU_012817_14_3_4"/>
    <dbReference type="OMA" id="LMTHVEN"/>
    <dbReference type="OrthoDB" id="1533572at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid pMOL30"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009279">
        <property type="term" value="C:cell outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015562">
        <property type="term" value="F:efflux transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046686">
        <property type="term" value="P:response to cadmium ion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003423">
        <property type="entry name" value="OMP_efflux"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02321">
        <property type="entry name" value="OEP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0105">Cadmium resistance</keyword>
    <keyword id="KW-0998">Cell outer membrane</keyword>
    <keyword id="KW-0170">Cobalt</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-0614">Plasmid</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0732">Signal</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1134">Transmembrane beta strand</keyword>
    <keyword id="KW-0813">Transport</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000021056" description="Cobalt-zinc-cadmium resistance protein CzcC">
        <location>
            <begin position="23"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="sequence conflict" description="In Ref. 1." evidence="3" ref="1">
        <original>ELGGKRSARINAAER</original>
        <variation>RLAASARLVSMPRKG</variation>
        <location>
            <begin position="106"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="sequence conflict" description="In Ref. 1; CAA67082." evidence="3" ref="1">
        <location>
            <position position="174"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000269" key="2">
        <source>
            <dbReference type="PubMed" id="7766206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="418" mass="44807" checksum="0DD60AD3B79817ED" modified="2006-07-11" version="4" precursor="true">MRRLFLPLGLAVAFLSPNFAVAQSDTGTSMVPVFPREAAGPLTLEAALSLAAGSNFNLSAAAKELDSTEGGIMQARVIPNPELKTLVEDTRKSTRTSTAQMNIPIELGGKRSARINAAERTRELAQATLAGVRGDIRAQVIESFFSVLIAQERVKLATGSADIAARGAQAASRRVAAGKISPVDETKARVEQANAELELAEATASLQSARQALTALWGNASPQFAEAQGNLDALPSRPAPELLQKELENSPLVAASRAELDRRQALVGVERSRQYPDLTVSLGAKRDTEANRNMAVIGVAIPLPIFDRNQGNLYSAIRQADKAQDEYLANRISLTRNLLMASNQLSVSRASAQTLKQTVLPGAEQAFNAATIGFEAGKFNYLDVLDAQRTLFQARIRYLGVLGQTYQAATTIDRILGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="49" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LA85</accession>
    <name>Q1LA85_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_6082</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="1 2">
        <name>pMOL30</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid pMOL30</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000354">
        <property type="protein sequence ID" value="ABF12941.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF12941">
        <property type="protein sequence ID" value="ABF12941"/>
        <property type="gene ID" value="Rmet_6082"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6082"/>
    <dbReference type="HOGENOM" id="CLU_727355_0_0_4"/>
    <dbReference type="OMA" id="EGKSHIA"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid pMOL30"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029470">
        <property type="entry name" value="PDDEXK_4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14281">
        <property type="entry name" value="PDDEXK_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF12941.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="380" mass="42282" checksum="0DE661C80E1F9F6C" modified="2006-05-30" version="1">MESPELNFYAVGGAGYLENPTSDLMALFMGAEPQAPRWLAKALLSCLANRGLAEGIIVESVDWDGVTAEREVAQTDQESDSVKRLDLVISDGDFVLGIENKVYATARGNPFHVYDRLLAERAAGGPIIKCVLRPTDRTSDVPLGWPVVTYPDLVKTALDLLGQEFVRAPVSKWHFFYREFLNHLESLANPESGTIMSDAAFSFALKNFHALSQAADLLGEFKSQLEHDATARVRERLSAMTLEGSVALTSKQQVWRSLNQHVVEIYPASWGKYSKVVLAYGEDETDEDHALYFYVLGYISFGTEKQSIEEIKQRFYADTKNEVSSWRTHSDPADNLFEEGRSTKYLGISGMPKDATLSGALQALEDLAAWMQQFVFTPAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="102" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LAP9</accession>
    <name>Q1LAP9_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Transcriptional regulator, IclR family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Rmet_5918</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="6">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="6">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12777.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5918"/>
    <dbReference type="EnsemblBacteria" id="ABF12777">
        <property type="protein sequence ID" value="ABF12777"/>
        <property type="gene ID" value="Rmet_5918"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5918"/>
    <dbReference type="eggNOG" id="COG1414">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_062618_0_1_4"/>
    <dbReference type="OMA" id="ARIHETC"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029016">
        <property type="entry name" value="GAF-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014757">
        <property type="entry name" value="Tscrpt_reg_IclR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005471">
        <property type="entry name" value="Tscrpt_reg_IclR_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09339">
        <property type="entry name" value="HTH_IclR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01614">
        <property type="entry name" value="IclR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00346">
        <property type="entry name" value="HTH_ICLR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51077">
        <property type="entry name" value="HTH_ICLR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51078">
        <property type="entry name" value="ICLR_ED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0614">Plasmid</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH iclR-type" evidence="3">
        <location>
            <begin position="53"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="domain" description="IclR-ED" evidence="4">
        <location>
            <begin position="114"/>
            <end position="298"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABF12777.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="298" mass="32440" checksum="398DBCA05B8B88EC" modified="2006-05-30" version="1">MRDYRTLPRTVLSAMDAPSPSPISLKPAQADTVRAAKPPADILGSFAGDPNFMLSLARGLTVLEAFSERKRPLTISQVAQRTQLSRASVRRCLYTLEQLGYVSQQDGHFALRPRVLHLGHAFFSSSAIVSLAQPVLDSLSARVHETSALAILDGTDILYLVRSEVQRVLTHSLGMGSRLPAYCTSTGRLLLAHQPQPILEAFFDHAELRPRTLLTKISRPELEAAFQRARDMDYVLIDEELEPGLRAIAVPVRSQAGVVLAAISVSVKANKVSEGEMIARLLGPMREAAAAIGKMIGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="100" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LAR7</accession>
    <name>Q1LAR7_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Transcriptional regulator, GntR-family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Rmet_5900</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="6">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="6">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12759.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518107.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5900"/>
    <dbReference type="EnsemblBacteria" id="ABF12759">
        <property type="protein sequence ID" value="ABF12759"/>
        <property type="gene ID" value="Rmet_5900"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5900"/>
    <dbReference type="eggNOG" id="COG2186">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017584_9_1_4"/>
    <dbReference type="OMA" id="SFVVDFH"/>
    <dbReference type="OrthoDB" id="1875458at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07377">
        <property type="entry name" value="WHTH_GntR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.530">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011711">
        <property type="entry name" value="GntR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008920">
        <property type="entry name" value="TF_FadR/GntR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000524">
        <property type="entry name" value="Tscrpt_reg_HTH_GntR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07729">
        <property type="entry name" value="FCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00392">
        <property type="entry name" value="GntR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00035">
        <property type="entry name" value="HTHGNTR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00895">
        <property type="entry name" value="FCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00345">
        <property type="entry name" value="HTH_GNTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48008">
        <property type="entry name" value="SSF48008"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50949">
        <property type="entry name" value="HTH_GNTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0614">Plasmid</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH gntR-type" evidence="4">
        <location>
            <begin position="7"/>
            <end position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABF12759.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="232" mass="26035" checksum="69845A7E8DA6206F" modified="2006-05-30" version="1">MGAGNARNRVEDIMRRMETALLDGTWPVGTRLPAERVLAEQFGVARNTIREAIQRLAARNLLQSRRGAGVYVTDQLRTGIASPWGQLVADHPALRDDILEFRRVLEGATAYFAALRADAADLKRIRALLKELERSRMTDDKRAEADADAKLHDAIAQASHNTMFLHLHTSVIGMLREHITINGTGLREADDESSDLLLRQHRTLCEAILARRPEEARTAMQTHIDFVRSKVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LAX4</accession>
    <name>Q1LAX4_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Mannose-1-phosphate guanylyltransferase</fullName>
            <ecNumber evidence="3">2.7.7.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">manC</name>
        <name evidence="9" type="ordered locus">Rmet_5843</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="10">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="10">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>alpha-D-mannose 1-phosphate + GTP + H(+) = diphosphate + GDP-alpha-D-mannose</text>
            <dbReference type="Rhea" id="RHEA:15229"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:57527"/>
            <dbReference type="ChEBI" id="CHEBI:58409"/>
            <dbReference type="EC" id="2.7.7.13"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the mannose-6-phosphate isomerase type 2 family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.13" evidence="3"/>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12702.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518051.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5843"/>
    <dbReference type="EnsemblBacteria" id="ABF12702">
        <property type="protein sequence ID" value="ABF12702"/>
        <property type="gene ID" value="Rmet_5843"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5843"/>
    <dbReference type="eggNOG" id="COG0662">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG0836">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035527_1_0_4"/>
    <dbReference type="OMA" id="ELKKHDP"/>
    <dbReference type="OrthoDB" id="224217at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016853">
        <property type="term" value="F:isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004475">
        <property type="term" value="F:mannose-1-phosphate guanylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000271">
        <property type="term" value="P:polysaccharide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006375">
        <property type="entry name" value="Man1P_GuaTrfase/Man6P_Isoase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001538">
        <property type="entry name" value="Man6P_isomerase-2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005835">
        <property type="entry name" value="NTP_transferase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011051">
        <property type="entry name" value="RmlC_Cupin_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01050">
        <property type="entry name" value="MannoseP_isomer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00483">
        <property type="entry name" value="NTP_transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51182">
        <property type="entry name" value="SSF51182"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="SSF53448"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01479">
        <property type="entry name" value="GMP_PMI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0413">Isomerase</keyword>
    <keyword evidence="5 9" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="9" id="KW-0614">Plasmid</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="NTP_transferase" evidence="7">
        <location>
            <begin position="14"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="domain" description="MannoseP_isomer" evidence="8">
        <location>
            <begin position="343"/>
            <end position="493"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001083"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU004190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01050"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ABF12702.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="498" mass="54048" checksum="D1A2646615175D9B" modified="2006-05-30" version="1">MPVIQPVKRPVIQPVILAGGAGTRLWPLSREQYPKQLLNLVANDTLLEATAARLEGVAARTGRPAGVEAPQIVVCGEEHRFMVADMLKRKGKRARVVSEPCGRNTAPALTMAALHAMASAGEGGDAVLVATPADHSVTDSDAFRDAIAAAVLHAERGAIVTLGVRPTAPETGFGYIRAAVPVPPGGFLIERFVEKPHLELAQHYVDSGEYWWNCGIFVVLASVWLKAVMQLQPEMHARCCAAYETGQPSESGKDDVFRIGQEAFDTCPSDSIDYAVMEKLDGLRGIPGMLVPMDAGWSDVGSWAAIWDIATKDNAGNAGRGRVLFDGASSCLAHSEGRLIACVGVQDVVVVETADAVLVVDKHHVQDVKNVVQRLRQEHGAEIVNHRKVHRPWGCYDSIDHGDRFQVKRIVVEPGASLSLQMHYHRAEHWVVVRGTARVTCGETTSILTENQSTYIPIGTLHRLENPGKTPLEIIEVQSGAYLGEDDIVRVEDNYGRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LB12</accession>
    <name>Q1LB12_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative major facilitator superfamily (MFS) transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_5805</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12664.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011520200.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5805"/>
    <dbReference type="EnsemblBacteria" id="ABF12664">
        <property type="protein sequence ID" value="ABF12664"/>
        <property type="gene ID" value="Rmet_5805"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5805"/>
    <dbReference type="eggNOG" id="COG0477">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_034180_11_0_4"/>
    <dbReference type="OMA" id="TPDDMRG"/>
    <dbReference type="OrthoDB" id="460405at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010290">
        <property type="entry name" value="TM_effector"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05977">
        <property type="entry name" value="MFS_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="SSF103473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="25"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="53"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="85"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="115"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="149"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="178"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="227"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="264"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="294"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="376"/>
            <end position="401"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF12664.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="419" mass="44568" checksum="96ACE2666F230E6F" modified="2006-05-30" version="1">MSTAPAQAADEPQTVFRDPAFRHFWFARLCTTIAYQIFTVAVGWQMYDLTRDPFMLGMVGLVQFLPSIVLLLMSGHVADRFDRRIVVRTCQVLEALIAALMAAASFSGWVTSEHIFLFVAAIGGCRAFETPTLQALLPSVVTSRQLPRAVALSSSAGQTAIIIGPALGGFAYVAGPGVVYSISAALFAIAAILVTTLKLRQAAQRMTAQASLKTLFAGFAYIRSHQVLLGAVSLDLFAVLLGGATALLPIYARDILHTGPWGLGLLRSAPAIGALTTALWLARNPLNRRVGRVMFTAVAIFGLATMVFGVSKWLPLSMAALVVLGASDMISVVVRQTLVQLDTPDDMRGRVGAVNSVFIGASNQLGEFESGVTAALLGPVGAVLLGGVGTLIVVVAWMRLFPNLTERDRLHDHHPAKAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LBL0</accession>
    <name>Q1LBL0_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Permease of the major facilitator superfamily</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Rmet_5607</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="9">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="9">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12466.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011520014.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5607"/>
    <dbReference type="EnsemblBacteria" id="ABF12466">
        <property type="protein sequence ID" value="ABF12466"/>
        <property type="gene ID" value="Rmet_5607"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5607"/>
    <dbReference type="eggNOG" id="COG0477">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001265_39_5_4"/>
    <dbReference type="OMA" id="DFFVYGI"/>
    <dbReference type="OrthoDB" id="446363at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="SSF103473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00216">
        <property type="entry name" value="SUGAR_TRANSPORT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0614">Plasmid</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="43"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="67"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="102"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="166"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="205"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="255"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="291"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="320"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="346"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="381"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="407"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="domain" description="MFS" evidence="7">
        <location>
            <begin position="28"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ABF12466.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="439" mass="47324" checksum="2F5C09F22E519078" modified="2006-05-30" version="1">MASLSHQHRASPTAPPAPGHHDVAPAEIATGVVIGRASEYFDFFTYGIASVLVFPSVYFPFASELAGLLYSFAIFSFAFVGRPFGTTLFMRIQRRWGRGIKLTASLFLLGTATVGIAFLPSYASIGSSAIYLLALFRFLQGIAFGGSWDGLPSLLALNAPENKRGWYAMVGQLGAPTGFIVAGALFLFLRSSLTPEEFIDWGWRYPFYVAFAINVVALFARLRLVVTHEYTRLLEEDELEPISTLQMVNAQGFNIFLGAFAALASYALFHLVTVFPLSWVSLHKTQNITDVLAVQIVGAVIAFIGTLISGWLADRIGRRTTLATMAVLIGIFSLFSPWLLDGGATGQNLFILIGFGLLGLSYGQAAGVVTSNFETRYRYTGAALTTDFAWLFGAAFAPLVALGLSSLFGLVAVSLYLLSGVVGTLVALRINRALGTPDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-10-07" version="55" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LC41</accession>
    <name>Q1LC41_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_5426</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12285.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF12285">
        <property type="protein sequence ID" value="ABF12285"/>
        <property type="gene ID" value="Rmet_5426"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5426"/>
    <dbReference type="eggNOG" id="ENOG50347DP">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_193003_0_0_4"/>
    <dbReference type="OMA" id="ISEYMNG"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="53"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF12285.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="83" mass="9100" checksum="10031097F0F44163" modified="2006-05-30" version="1">MNTSTTPFTVSVYPIQQEPGVWFANYMISEYRNGAERVVANVSMRHATHCSEAKAKQAARQAGKSAAARMQRSAQRRAPSGQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LCW2</accession>
    <name>Q1LCW2_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Short-chain dehydrogenase/reductase SDR</fullName>
            <ecNumber evidence="1">1.1.1.35</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">hadH</name>
        <name evidence="1" type="ordered locus">Rmet_5155</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EC" id="1.1.1.35" evidence="1"/>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12014.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519560.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5155"/>
    <dbReference type="EnsemblBacteria" id="ABF12014">
        <property type="protein sequence ID" value="ABF12014"/>
        <property type="gene ID" value="Rmet_5155"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5155"/>
    <dbReference type="eggNOG" id="COG1028">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010194_42_0_4"/>
    <dbReference type="OMA" id="WAQRTIG"/>
    <dbReference type="OrthoDB" id="1260864at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003857">
        <property type="term" value="F:3-hydroxyacyl-CoA dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00061">
        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF12014.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="255" mass="26468" checksum="9BE94662FDE2B4FD" modified="2006-05-30" version="1">MKLDQTMSAVVTGGASGLGLASAKRLVEKGVKVVIADISEERAAAALDALGPNATFVQADVTDTDQMTRVFDAAVAMGPLRALVHCAGLGGAVRVVEKDGSPGSLEAYERVVRINLIGTFNVLRLGAARMATNEPVDGERGACVLTASVAAYEGQIGQIPYASAKAGIVGMTIVAARDLAQRMIRVCTIAPGLFDTPLLARLPDNVKASLGAMVPHPARLGAPDEYASTALHILENPMLNGETIRLDGAIRMAPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LDA1</accession>
    <name>Q1LDA1_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_5013</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11875.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519423.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5013"/>
    <dbReference type="EnsemblBacteria" id="ABF11875">
        <property type="protein sequence ID" value="ABF11875"/>
        <property type="gene ID" value="Rmet_5013"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5013"/>
    <dbReference type="eggNOG" id="COG2303">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008878_4_1_4"/>
    <dbReference type="OMA" id="WEQAPLA"/>
    <dbReference type="OrthoDB" id="365211at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016614">
        <property type="term" value="F:oxidoreductase activity, acting on CH-OH group of donors"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006076">
        <property type="entry name" value="FAD-dep_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007867">
        <property type="entry name" value="GMC_OxRtase_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01266">
        <property type="entry name" value="DAO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05199">
        <property type="entry name" value="GMC_oxred_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="SSF51905"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DAO" evidence="1">
        <location>
            <begin position="26"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="domain" description="GMC_oxred_C" evidence="2">
        <location>
            <begin position="389"/>
            <end position="516"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF05199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF11875.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="524" mass="58579" checksum="CE06D8F56177C218" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LDC1</accession>
    <name>Q1LDC1_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Membrane efflux protein (Major facilitator superfamily MFS_1)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Rmet_4993</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="9">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="9">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11855.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4993"/>
    <dbReference type="EnsemblBacteria" id="ABF11855">
        <property type="protein sequence ID" value="ABF11855"/>
        <property type="gene ID" value="Rmet_4993"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4993"/>
    <dbReference type="eggNOG" id="COG2814">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001265_46_6_4"/>
    <dbReference type="OMA" id="QIWIIWL"/>
    <dbReference type="OrthoDB" id="1121832at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="SSF103473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00216">
        <property type="entry name" value="SUGAR_TRANSPORT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0614">Plasmid</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="110"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="148"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="178"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="203"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="236"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="265"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="346"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="380"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="409"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="433"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="467"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="497"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="domain" description="MFS" evidence="7">
        <location>
            <begin position="112"/>
            <end position="521"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ABF11855.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="526" mass="56202" checksum="F8BE47ED6088E575" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="96" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LDE3</accession>
    <name>Q1LDE3_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Putative carbamoyl-phosphate synthase L chain, ATP-binding</fullName>
            <ecNumber evidence="13">2.1.3.-</ecNumber>
            <ecNumber evidence="13">6.3.4.14</ecNumber>
            <ecNumber evidence="13">6.4.1.1</ecNumber>
            <ecNumber evidence="13">6.4.1.2</ecNumber>
            <ecNumber evidence="13">6.4.1.3</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ordered locus">Rmet_4971</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="14">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="14">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>biotin</name>
            <dbReference type="ChEBI" id="CHEBI:57586"/>
        </cofactor>
    </comment>
    <dbReference type="EC" id="2.1.3.-" evidence="13"/>
    <dbReference type="EC" id="6.3.4.14" evidence="13"/>
    <dbReference type="EC" id="6.4.1.1" evidence="13"/>
    <dbReference type="EC" id="6.4.1.2" evidence="13"/>
    <dbReference type="EC" id="6.4.1.3" evidence="13"/>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11833.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4971"/>
    <dbReference type="EnsemblBacteria" id="ABF11833">
        <property type="protein sequence ID" value="ABF11833"/>
        <property type="gene ID" value="Rmet_4971"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4971"/>
    <dbReference type="eggNOG" id="COG0439">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG4799">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009218_0_0_4"/>
    <dbReference type="OMA" id="GTQGMRN"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003989">
        <property type="term" value="F:acetyl-CoA carboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004075">
        <property type="term" value="F:biotin carboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004658">
        <property type="term" value="F:propionyl-CoA carboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004736">
        <property type="term" value="F:pyruvate carboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006164">
        <property type="term" value="P:purine nucleotide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034733">
        <property type="entry name" value="AcCoA_carboxyl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011761">
        <property type="entry name" value="ATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013815">
        <property type="entry name" value="ATP_grasp_subdomain_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005481">
        <property type="entry name" value="BC-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001882">
        <property type="entry name" value="Biotin_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011764">
        <property type="entry name" value="Biotin_carboxylation_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005482">
        <property type="entry name" value="Biotin_COase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000089">
        <property type="entry name" value="Biotin_lipoyl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005479">
        <property type="entry name" value="CbamoylP_synth_lsu-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029045">
        <property type="entry name" value="ClpP/crotonase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011763">
        <property type="entry name" value="COA_CT_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016185">
        <property type="entry name" value="PreATP-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011054">
        <property type="entry name" value="Rudment_hybrid_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011053">
        <property type="entry name" value="Single_hybrid_motif"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02785">
        <property type="entry name" value="Biotin_carb_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00289">
        <property type="entry name" value="Biotin_carb_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00364">
        <property type="entry name" value="Biotin_lipoyl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01039">
        <property type="entry name" value="Carboxyl_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02786">
        <property type="entry name" value="CPSase_L_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00878">
        <property type="entry name" value="Biotin_carb_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51230">
        <property type="entry name" value="SSF51230"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51246">
        <property type="entry name" value="SSF51246"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52096">
        <property type="entry name" value="SSF52096"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52440">
        <property type="entry name" value="SSF52440"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50975">
        <property type="entry name" value="ATP_GRASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50979">
        <property type="entry name" value="BC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00188">
        <property type="entry name" value="BIOTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50968">
        <property type="entry name" value="BIOTINYL_LIPOYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50989">
        <property type="entry name" value="COA_CT_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00867">
        <property type="entry name" value="CPSASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 8 13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0092">Biotin</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 13" id="KW-0436">Ligase</keyword>
    <keyword evidence="4 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-0614">Plasmid</keyword>
    <keyword evidence="5" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Biotin carboxylation" evidence="11">
        <location>
            <begin position="44"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="domain" description="ATP-grasp" evidence="10">
        <location>
            <begin position="162"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="domain" description="Lipoyl-binding" evidence="9">
        <location>
            <begin position="539"/>
            <end position="617"/>
        </location>
    </feature>
    <feature type="domain" description="CoA carboxyltransferase C-terminal" evidence="12">
        <location>
            <begin position="897"/>
            <end position="1152"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ABF11833.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="1152" mass="123359" checksum="9D3700F82B72C0A7" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LDG8</accession>
    <name>Q1LDG8_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Citrate synthase (unknown stereospecificity)</fullName>
            <ecNumber evidence="2">2.3.3.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_4946</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Carbohydrate metabolism; tricarboxylic acid cycle; isocitrate from oxaloacetate: step 1/2.</text>
    </comment>
    <dbReference type="EC" id="2.3.3.16" evidence="2"/>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11808.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519367.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4946"/>
    <dbReference type="EnsemblBacteria" id="ABF11808">
        <property type="protein sequence ID" value="ABF11808"/>
        <property type="gene ID" value="Rmet_4946"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4946"/>
    <dbReference type="eggNOG" id="COG0372">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_070533_1_0_4"/>
    <dbReference type="OMA" id="FEIARDC"/>
    <dbReference type="OrthoDB" id="1412360at2"/>
    <dbReference type="UniPathway" id="UPA00223">
        <property type="reaction ID" value="UER00717"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036440">
        <property type="term" value="F:citrate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016143">
        <property type="entry name" value="Citrate_synth-like_sm_a-sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002020">
        <property type="entry name" value="Citrate_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036969">
        <property type="entry name" value="Citrate_synthase_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00285">
        <property type="entry name" value="Citrate_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48256">
        <property type="entry name" value="SSF48256"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004751"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012972"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF11808.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="252" mass="26448" checksum="39AD3B347CC74A7E" modified="2006-05-30" version="1">MLNSAIGGGDADHIKVRGYDLVDDLMDQRDFIDVLCLEILGDFPDANLKRMINMFLVTSSDHGLTPSALSTRLTLHGAPESLQGAVAAGILGAGSRFLGTVEYSARFLAGVATDVGADWTAEHMRAAAEAAVARSRASGKRIPGIGHPIHVGGDPRCIKLLKVADECGYQGRHCALAQEIAAAATRAAGKPVPLNATGAKGAILLDMGMSPEFGKGLTLIGRVAGLVAHVIEEREHPIAQDIWDMATAQSHR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LDN7</accession>
    <name>Q1LDN7_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 7">Muconolactone Delta-isomerase</fullName>
            <shortName evidence="7">MIase</shortName>
            <ecNumber evidence="5 7">5.3.3.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">catC</name>
        <name evidence="9" type="ordered locus">Rmet_4877</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="10">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="10">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 7">
            <text>(S)-muconolactone = (4,5-dihydro-5-oxofuran-2-yl)-acetate</text>
            <dbReference type="Rhea" id="RHEA:12348"/>
            <dbReference type="ChEBI" id="CHEBI:58425"/>
            <dbReference type="ChEBI" id="CHEBI:58736"/>
            <dbReference type="EC" id="5.3.3.4"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 7">Aromatic compound metabolism; beta-ketoadipate pathway; 5-oxo-4,5-dihydro-2-furylacetate from catechol: step 3/3.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homodecamer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 7">Belongs to the muconolactone Delta-isomerase family.</text>
    </comment>
    <dbReference type="EC" id="5.3.3.4" evidence="5 7"/>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11739.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008641477.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4877"/>
    <dbReference type="EnsemblBacteria" id="ABF11739">
        <property type="protein sequence ID" value="ABF11739"/>
        <property type="gene ID" value="Rmet_4877"/>
    </dbReference>
    <dbReference type="GeneID" id="60823869"/>
    <dbReference type="KEGG" id="rme:Rmet_4877"/>
    <dbReference type="eggNOG" id="COG4829">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_080702_2_0_4"/>
    <dbReference type="OMA" id="WRVPGRN"/>
    <dbReference type="OrthoDB" id="1857318at2"/>
    <dbReference type="UniPathway" id="UPA00157">
        <property type="reaction ID" value="UER00260"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016159">
        <property type="term" value="F:muconolactone delta-isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042952">
        <property type="term" value="P:beta-ketoadipate pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011008">
        <property type="entry name" value="Dimeric_a/b-barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026029">
        <property type="entry name" value="MLI_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003464">
        <property type="entry name" value="Muconolactone_d_Isoase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02426">
        <property type="entry name" value="MIase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001486">
        <property type="entry name" value="CatC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54909">
        <property type="entry name" value="SSF54909"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03221">
        <property type="entry name" value="muco_delta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0058">Aromatic hydrocarbons catabolism</keyword>
    <keyword evidence="7 9" id="KW-0413">Isomerase</keyword>
    <keyword evidence="9" id="KW-0614">Plasmid</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MIase" evidence="8">
        <location>
            <begin position="1"/>
            <end position="90"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001739"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011365"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022797"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR001486"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02426"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ABF11739.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="92" mass="10626" checksum="90313C340B6AE5EA" modified="2006-05-30" version="1">MLFLVRMDVQIPHDLPAAEADEIKAREKAYSQDLQRQGKWQALYRVVGEYANYSLFDVESNDELHTLLSALPLFPYMKIHVTPLAKHPSSIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="107" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LDT4</accession>
    <name>Q1LDT4_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Sensor protein QseC</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">qseC</name>
        <name evidence="8" type="ordered locus">Rmet_4830</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="9">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="9">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell inner membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11692.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519255.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4830"/>
    <dbReference type="EnsemblBacteria" id="ABF11692">
        <property type="protein sequence ID" value="ABF11692"/>
        <property type="gene ID" value="Rmet_4830"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4830"/>
    <dbReference type="eggNOG" id="COG2205">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_89_37_4"/>
    <dbReference type="OMA" id="DKLFDTQ"/>
    <dbReference type="OrthoDB" id="692375at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00082">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013727">
        <property type="entry name" value="2CSK_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003661">
        <property type="entry name" value="HisK_dim/P"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036097">
        <property type="entry name" value="HisK_dim/P_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08521">
        <property type="entry name" value="2CSK_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00512">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00388">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47384">
        <property type="entry name" value="SSF47384"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="SSF55874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0418">Kinase</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0614">Plasmid</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Histidine kinase" evidence="7">
        <location>
            <begin position="242"/>
            <end position="455"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004429"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00017234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ABF11692.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="457" mass="49976" checksum="1D95636145EBE3A9" modified="2006-05-30" version="1">MTLRRRLILAVLAAVTFAWVLTSALIYLSAQEEINELYDTAMVRMAQQMQALLPRVHTQTSPAPPAANGPIPGDADLVDEGSAGLGDLAIAAWRPDGEPLHIDPDGDHLPRLPDVQGFTERKIDGVPWRLYYLNDPAQGWRVCVGQVKAERGELIVAYLQAQVLPWVFGLPLLTTLLLWGVSRALHPVLTLSQAIAARVPGDPTPLTLHEVPRELVPLVTEMNKLLSRATALLEHERRLTADAAHELRTPLAALKAQWEVARRSDDPTERSRAAANVEAGIDGMSRLVSQLLTMSRLEDEEAPPTRGPVDWREVSRQVLSDCLLLSERRNVDMEVLWPPAGQQPLPVNGDPTLLGTMLRNLMDNAIRYSPAGTVVTLAFEPDRITVSDRGPGVAPELLRRLGDRFFRAAGQQEQGNGLGISIARRVAVLHGLALEFANRQAGDGSGLVAAIRLNGMY</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-12-02" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LDX7</accession>
    <name>Q1LDX7_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative amidase</fullName>
            <ecNumber evidence="2">3.5.1.4</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_4787</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EC" id="3.5.1.4" evidence="2"/>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11649.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4787"/>
    <dbReference type="EnsemblBacteria" id="ABF11649">
        <property type="protein sequence ID" value="ABF11649"/>
        <property type="gene ID" value="Rmet_4787"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4787"/>
    <dbReference type="eggNOG" id="COG0154">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009600_0_4_4"/>
    <dbReference type="OMA" id="DRSDWYR"/>
    <dbReference type="OrthoDB" id="1239251at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004040">
        <property type="term" value="F:amidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043864">
        <property type="term" value="F:indoleacetamide hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1300.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000120">
        <property type="entry name" value="Amidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020556">
        <property type="entry name" value="Amidase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023631">
        <property type="entry name" value="Amidase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036928">
        <property type="entry name" value="AS_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11895">
        <property type="entry name" value="PTHR11895"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01425">
        <property type="entry name" value="Amidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75304">
        <property type="entry name" value="SSF75304"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00571">
        <property type="entry name" value="AMIDASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Amidase" evidence="1">
        <location>
            <begin position="44"/>
            <end position="481"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF11649.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="505" mass="54982" checksum="781D225A2BEF57F7" modified="2006-05-30" version="1">MMPSSATSNLRPPPMSAYRPDPIVMLSARQLSEAIHKKEVSSREVMTAYLAHIARVNPGSNAIVAMQDEEALMREASAADEALAAGKSSGWMHGFPQAPKDLAMTRGIRTTYGSPIFRDNIPASDSVIVERMRRAGSILIGKTNTPEFGLGSHTFNPVYGATLNPYDPTRSAGGSSGGAAAALALRMLPVADGSDFGGSLRNPAAFCNVYGFRPSAGRVPYGPTPELFLPQLGSEGPMARNVEDLALLLATQSGPDARTPQALPHDPMLASLTPANVADILKTDLTGKRVAWLGDWGGYLPMEDGILSLCEQALRFFPDFGVDVEAIKAPYPPEKIWQTWLVYRHFLTGNSRLGLYTNPSTRAQLKSEAIWEIEGSLDLKASDLYAASTDRSAWYQALQRTFTQFDYIAVPTAQVFPFDVTQHWPKQIADRQMDTYHRWMEVVVAWTLSGCPVISVPVGFSADGLPMGMQLIGRPRDDLGVLQLAAAYEKVRDWVNDRLPPAIEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LE53</accession>
    <name>Q1LE53_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Type II secretion system protein E</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">gspE</name>
        <name evidence="3" type="ordered locus">Rmet_4711</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the GSP E family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11573.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519144.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4711"/>
    <dbReference type="EnsemblBacteria" id="ABF11573">
        <property type="protein sequence ID" value="ABF11573"/>
        <property type="gene ID" value="Rmet_4711"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4711"/>
    <dbReference type="eggNOG" id="COG2804">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013446_10_5_4"/>
    <dbReference type="OMA" id="DIHLECR"/>
    <dbReference type="OrthoDB" id="749544at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001482">
        <property type="entry name" value="T2SS/T4SS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007831">
        <property type="entry name" value="T2SS_GspE_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00437">
        <property type="entry name" value="T2SSE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05157">
        <property type="entry name" value="T2SSE_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00662">
        <property type="entry name" value="T2SP_E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="T2SP_E" evidence="2">
        <location>
            <begin position="385"/>
            <end position="399"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006611"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS00662"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF11573.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="568" mass="61959" checksum="D2475B747B7D9CBD" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-10-07" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LEB0</accession>
    <name>Q1LEB0_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_4654</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11516.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4654"/>
    <dbReference type="EnsemblBacteria" id="ABF11516">
        <property type="protein sequence ID" value="ABF11516"/>
        <property type="gene ID" value="Rmet_4654"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4654"/>
    <dbReference type="HOGENOM" id="CLU_2438615_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="16"/>
            <end position="34"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF11516.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="90" mass="10001" checksum="839E511B23572965" modified="2006-05-30" version="1">MLVRTGLRILGWNRRVVAHVTFCLWCALLLCFVISRQRRMYRNGVSVLDLSARDAGGLAASGDGLGVLRHGRAVRNAVRRLRGKEKSPLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LEB1</accession>
    <name>Q1LEB1_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative metal-dependent amidohydrolase with the TIM-barrel fold</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_4653</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11515.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519090.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF11515">
        <property type="protein sequence ID" value="ABF11515"/>
        <property type="gene ID" value="Rmet_4653"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4653"/>
    <dbReference type="eggNOG" id="COG1574">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009942_1_0_4"/>
    <dbReference type="OMA" id="DIACHAI"/>
    <dbReference type="OrthoDB" id="751534at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016810">
        <property type="term" value="F:hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01300">
        <property type="entry name" value="YtcJ_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013108">
        <property type="entry name" value="Amidohydro_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011059">
        <property type="entry name" value="Metal-dep_hydrolase_composite"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033932">
        <property type="entry name" value="YtcJ-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07969">
        <property type="entry name" value="Amidohydro_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51338">
        <property type="entry name" value="SSF51338"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="SSF51556"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193064" evidence="1">
        <location>
            <begin position="24"/>
            <end position="560"/>
        </location>
    </feature>
    <feature type="domain" description="Amidohydro_3" evidence="2">
        <location>
            <begin position="73"/>
            <end position="551"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07969"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF11515.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="560" mass="61166" checksum="7C7F964FAE1A4DF4" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LEN5</accession>
    <name>Q1LEN5_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">ATPase, AAA_3 family MoxR-like ATPase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">moxR</name>
        <name evidence="3" type="ordered locus">Rmet_4526</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11391.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518984.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4526"/>
    <dbReference type="EnsemblBacteria" id="ABF11391">
        <property type="protein sequence ID" value="ABF11391"/>
        <property type="gene ID" value="Rmet_4526"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4526"/>
    <dbReference type="eggNOG" id="COG0714">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_034716_2_0_4"/>
    <dbReference type="OMA" id="NRFQMND"/>
    <dbReference type="OrthoDB" id="688156at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011703">
        <property type="entry name" value="ATPase_AAA-3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041628">
        <property type="entry name" value="ChlI/MoxR_AAA_lid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07726">
        <property type="entry name" value="AAA_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17863">
        <property type="entry name" value="AAA_lid_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA_3" evidence="1">
        <location>
            <begin position="37"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="domain" description="AAA_lid_2" evidence="2">
        <location>
            <begin position="254"/>
            <end position="318"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF07726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF17863"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF11391.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="326" mass="36040" checksum="EA27300909E0D667" modified="2006-05-30" version="1">MSARDDFLALQARMGETIVGQARMIERLLLGLLADGHLLVEGLPGLAKTRAIKSLAKHLDARLSRVQFTPDLLPADITGSDIYYSEGGKGEFRFQPGPLFANLILADEVNRSPAKVQSALLEAMEERQVTVGGNTHPLERLFLVMATQNPIEQEGTYPLPEAQMDRFLMHVNVDYPAADAEAEIIRLARAEEEPDTHQGDAGSSETRMSPATVFDARRQIHEIKVSEPVERYIVALVQATREPARLDDDLKRWIEVGVSPRGSIGLDKVARAMAWLRGRDYVTPEDVQAVVHDVFRHRLILSYDAHAAGVSANAVIDRLVQRVAVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LEZ1</accession>
    <name>Q1LEZ1_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">L-carnitine dehydratase/bile acid-inducible protein F</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">caiB</name>
        <name evidence="1" type="ordered locus">Rmet_4420</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11285.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518897.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF11285">
        <property type="protein sequence ID" value="ABF11285"/>
        <property type="gene ID" value="Rmet_4420"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4420"/>
    <dbReference type="eggNOG" id="COG1804">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033975_0_0_4"/>
    <dbReference type="OMA" id="FFWSPIN"/>
    <dbReference type="OrthoDB" id="969868at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1540.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10540">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003673">
        <property type="entry name" value="CoA-Trfase_fam_III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044855">
        <property type="entry name" value="CoA-Trfase_III_dom3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023606">
        <property type="entry name" value="CoA-Trfase_III_dom_1_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02515">
        <property type="entry name" value="CoA_transf_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89796">
        <property type="entry name" value="SSF89796"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF11285.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="413" mass="44538" checksum="14C5F84B40D3FA79" modified="2006-05-30" version="1">MQTHADTQTHPPGALAGLRVVDLTQMLAGPFCTQVLADHGADVIKVEAMTGDGTRITGPFCDDDTLREFGGYFQSVNRNKRSIAVDLKSSTGREILRKLIDSADVVVENFRAGVMERLGLSYETLRETNPRLVYGTVRGFGDPRSGESPYAQWPAYDVVSQAMGGMMGITGPDRDTATKIGPGVGDTIPALMLCIGILSAVHRARGTGHGQFVDVAMTDAVLAMCERIVYQTSYTGVVPAPEGNRHPLLCPFGLFRSRDGQVSIACATDAFWEKLAIAIGRPDMATDEAFATNAARVQHQQQVIDAIEAFTTVRTKEEIAAVLGGKVPFGPVYTAAEIFADPHYAVREMLVEVEQPGSARPVKIAGVPIKLSDTPGAVRRRAPLLGEHTEEILLATGYTERDIKGLRETKAVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="55" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LF06</accession>
    <name>Q1LF06_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_4405</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11270.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4405"/>
    <dbReference type="DNASU" id="4041263"/>
    <dbReference type="EnsemblBacteria" id="ABF11270">
        <property type="protein sequence ID" value="ABF11270"/>
        <property type="gene ID" value="Rmet_4405"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4405"/>
    <dbReference type="eggNOG" id="ENOG5032WTM">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_154423_0_0_4"/>
    <dbReference type="OMA" id="TYMRRWL"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="63"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="89"/>
            <end position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF11270.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="118" mass="13630" checksum="A7CB9E0C863FBE78" modified="2006-05-30" version="1">MVTALARPEQTMQELAFRTLLYRYFFFDWLFKDASRGDLRERSAAWRFNQAHAHWLLTYLRRWVWCGALFCLLGGSIELMLSAPVLSAFFYVPGVLSVPINVVICVLWVGLKTLPGPM</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LF41</accession>
    <name>Q1LF41_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Zinc-type alcohol dehydrogenase-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_4370</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the zinc-containing alcohol dehydrogenase family. Quinone oxidoreductase subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11235.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518852.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4370"/>
    <dbReference type="EnsemblBacteria" id="ABF11235">
        <property type="protein sequence ID" value="ABF11235"/>
        <property type="gene ID" value="Rmet_4370"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4370"/>
    <dbReference type="eggNOG" id="COG0604">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026673_3_0_4"/>
    <dbReference type="OMA" id="HICTIVE"/>
    <dbReference type="OrthoDB" id="1752728at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd08252">
        <property type="entry name" value="AL_MDR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013154">
        <property type="entry name" value="ADH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014182">
        <property type="entry name" value="ADH_Zn_typ-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020843">
        <property type="entry name" value="PKS_ER"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002364">
        <property type="entry name" value="Quin_OxRdtase/zeta-crystal_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08240">
        <property type="entry name" value="ADH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00829">
        <property type="entry name" value="PKS_ER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="SSF50129"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02817">
        <property type="entry name" value="adh_fam_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01162">
        <property type="entry name" value="QOR_ZETA_CRYSTAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="PKS_ER" evidence="2">
        <location>
            <begin position="13"/>
            <end position="335"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU364000"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF11235.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="338" mass="35825" checksum="38D497F27F3A3CD8" modified="2006-05-30" version="1">MKAIGLTRYLPISDPQSLVDVEIAQPEASGHDLLVKVEAISVNPVDTKVRAPKDKVEDAPRVLGWDAAGTVVAVGADVTLFKVGDPVFYAGSITRPGANSEYHLVDERIVGHKPASLDFPQAAALPLTAITAWEAMFDRLRISPKGEDAGKSVLIIGGAGGVGSIGIQLAKVLAGLTVIATASRPESEAWCRGLGADHTIDHHGDIPAQLRALGFAHVDFVLCFNDTDRHFNAMAEAIAPQGTICTIVENTGPLAISALKNKSAGFVWELMFTRAMFQTPDIIAQHRLLNEVARLIDAGKLRTTVGDNLGTINAANLRRAHALLEGGRTIGKLVLSGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LF92</accession>
    <name>Q1LF92_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Short-chain dehydrogenase/reductase SDR</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_4319</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11184.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518808.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4319"/>
    <dbReference type="EnsemblBacteria" id="ABF11184">
        <property type="protein sequence ID" value="ABF11184"/>
        <property type="gene ID" value="Rmet_4319"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4319"/>
    <dbReference type="eggNOG" id="COG1028">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010194_1_2_4"/>
    <dbReference type="OMA" id="CIEYLIS"/>
    <dbReference type="OrthoDB" id="1601931at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00061">
        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF11184.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="239" mass="25204" checksum="B23FB2153CFAE87C" modified="2006-05-30" version="1">MSHKTFLITGASKGIGRALSHRLAAAGHKVIGVARNADPGFPGILVPIDLTDPVATEHALTTLRSEHVIDGVVNNVGFIELAPLGNIDLDAMDDMFRSNLRPAVQLVQALLPDMRERGWGRIVNLSSLVVLGVANRSVYAAAKSAMISFTRTWALELAPTGITVNAVAPGPVETEMFRANTPQGSDAEKRFLSMVPMGRLGKPDELAAAIEFLLSEPAGFITGQTLFVDGGASIGKAAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-10-07" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LFN3</accession>
    <name>Q1LFN3_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Transcriptional regulator, LysR family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_4176</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the LysR transcriptional regulatory family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11043.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4176"/>
    <dbReference type="EnsemblBacteria" id="ABF11043">
        <property type="protein sequence ID" value="ABF11043"/>
        <property type="gene ID" value="Rmet_4176"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4176"/>
    <dbReference type="eggNOG" id="COG0583">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039613_1_1_4"/>
    <dbReference type="OMA" id="LEITIGM"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005119">
        <property type="entry name" value="LysR_subst-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000847">
        <property type="entry name" value="Tscrpt_reg_HTH_LysR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00126">
        <property type="entry name" value="HTH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03466">
        <property type="entry name" value="LysR_substrate"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00039">
        <property type="entry name" value="HTHLYSR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50931">
        <property type="entry name" value="HTH_LYSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH lysR-type" evidence="2">
        <location>
            <begin position="15"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF11043.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="298" mass="32436" checksum="66E9F50C8062F400" modified="2006-05-30" version="1">MLTAIGFTDDNAAMLNPVWIQTFATVATARSFTDAGRQLGLSQSSVSDHIRRLEGSVSRRLFVRDTHSLALTPDGEALLVHARLILESLARAESQFSGPRLQGRVRLGTSEDLAQGPLPNVLAAFRATHPDVELEITIGMTSKLYEWMEAGTLDLIVGKRREGERRGVPLFRGRLEWLARPGTVVDVRQPLPLILVNEPSVTRSVVLDTLAKNGWRWRVVCTSSSHSGCIAAARGGLGITVRAENLTGGGLVPPVNFDALPALPDVEFITLAARRLTEPADTLLQLIRKSDLRAARVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LFQ2</accession>
    <name>Q1LFQ2_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">pelC</name>
        <name evidence="2" type="ordered locus">Rmet_4157</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11024.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518646.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4157"/>
    <dbReference type="EnsemblBacteria" id="ABF11024">
        <property type="protein sequence ID" value="ABF11024"/>
        <property type="gene ID" value="Rmet_4157"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4157"/>
    <dbReference type="eggNOG" id="COG5616">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_105029_0_0_4"/>
    <dbReference type="OMA" id="NYTETPQ"/>
    <dbReference type="OrthoDB" id="1498484at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193098" evidence="1">
        <location>
            <begin position="31"/>
            <end position="182"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF11024.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="182" mass="19316" checksum="5CCBEB223D4D9AE6" modified="2006-05-30" version="1">MNRMTTRMGRVMAWCGALGAALWLAGCAVTDVGRAPQMSPGDVVAVLPIVNNTETPQAGLRAEAIAESLLKTGGVANLKRYPASLNPETLFEPAEREAVGKALEWARGERARYALTGSVQEWRYKVGVDGEPAVGISLQLIDVASGQVVWSATGSNAGWSRESLSGVAQKLLRRLLAPLMQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="102" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LFS7</accession>
    <name>Q1LFS7_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex</fullName>
            <ecNumber evidence="4">2.3.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Rmet_4132</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="8">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="8">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 4">
            <name>(R)-lipoate</name>
            <dbReference type="ChEBI" id="CHEBI:83088"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 4">Belongs to the 2-oxoacid dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.-" evidence="4"/>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10999.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518624.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4132"/>
    <dbReference type="EnsemblBacteria" id="ABF10999">
        <property type="protein sequence ID" value="ABF10999"/>
        <property type="gene ID" value="Rmet_4132"/>
    </dbReference>
    <dbReference type="GeneID" id="60823284"/>
    <dbReference type="KEGG" id="rme:Rmet_4132"/>
    <dbReference type="eggNOG" id="COG0508">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016733_10_0_4"/>
    <dbReference type="OMA" id="RAMAQNM"/>
    <dbReference type="OrthoDB" id="1626282at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016746">
        <property type="term" value="F:acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.559.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.320.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003016">
        <property type="entry name" value="2-oxoA_DH_lipoyl-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001078">
        <property type="entry name" value="2-oxoacid_DH_actylTfrase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000089">
        <property type="entry name" value="Biotin_lipoyl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023213">
        <property type="entry name" value="CAT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036625">
        <property type="entry name" value="E3-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004167">
        <property type="entry name" value="PSBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011053">
        <property type="entry name" value="Single_hybrid_motif"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00198">
        <property type="entry name" value="2-oxoacid_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00364">
        <property type="entry name" value="Biotin_lipoyl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02817">
        <property type="entry name" value="E3_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47005">
        <property type="entry name" value="SSF47005"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51230">
        <property type="entry name" value="SSF51230"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50968">
        <property type="entry name" value="BIOTINYL_LIPOYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00189">
        <property type="entry name" value="LIPOYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51826">
        <property type="entry name" value="PSBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="3 4" id="KW-0450">Lipoyl</keyword>
    <keyword evidence="7" id="KW-0614">Plasmid</keyword>
    <keyword evidence="7" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Lipoyl-binding" evidence="5">
        <location>
            <begin position="1"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="domain" description="Peripheral subunit-binding (PSBD)" evidence="6">
        <location>
            <begin position="117"/>
            <end position="154"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001938"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003423"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABF10999.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="377" mass="39388" checksum="4247903E6AC8BCA5" modified="2006-05-30" version="1">MRIFKLPDLGEGLQEAEIVNWHVKPGDTIAADQPLLSVETAKAIVEIPSPFAGQVAKLFAQPGDIVHLGAPLVGFEGAGASDDAGTVVGAVKVGTHVVNEGVTRVGAAGIPGGHGIKATPAVRALARKLSVDLSMVTPSGHDGVITATDVQRVATTLADVGAPDVLRGVRRAMAQNMARAQSEVAAATVMDDADLHAWQATGASVTGQDITIRLVRALVAGVRAEPALNAWYEGRTGRRHLLERIDVGIAADLPEGLFVPVLRNVGKRDAADLRAGLDRMRADVVARTIAPEEMRGNTITLSNFGMIAGRYAAPIVVPPTVAILGAGRVRDEVVASGGVPAVHRVMPLSLTFDHRVVTGGEAARFLRAVIEDLERPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LG00</accession>
    <name>Q1LG00_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 8">Heme exporter protein C</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Cytochrome c-type biogenesis protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8 10" type="primary">ccmC</name>
        <name evidence="10" type="ordered locus">Rmet_4058</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="11">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="11">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 8">Required for the export of heme to the periplasm for the biogenesis of c-type cytochromes.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cell inner membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 8">Belongs to the CcmC/CycZ/HelC family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10926.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518554.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4058"/>
    <dbReference type="EnsemblBacteria" id="ABF10926">
        <property type="protein sequence ID" value="ABF10926"/>
        <property type="gene ID" value="Rmet_4058"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4058"/>
    <dbReference type="eggNOG" id="COG0755">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_066538_2_1_4"/>
    <dbReference type="OMA" id="CGIIALW"/>
    <dbReference type="OrthoDB" id="955123at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015232">
        <property type="term" value="F:heme transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017004">
        <property type="term" value="P:cytochrome complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002541">
        <property type="entry name" value="Cyt_c_assembly"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003557">
        <property type="entry name" value="Cyt_c_biogenesis_CcmC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045062">
        <property type="entry name" value="Cyt_c_biogenesis_CcsA/CcmC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30071">
        <property type="entry name" value="PTHR30071"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01578">
        <property type="entry name" value="Cytochrom_C_asm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01386">
        <property type="entry name" value="CCMCBIOGNSIS"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01191">
        <property type="entry name" value="ccmC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="8" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-0201">Cytochrome c-type biogenesis</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0614">Plasmid</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="8" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="37"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="79"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="112"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="143"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="173"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="213"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="domain" description="Cytochrom_C_asm" evidence="9">
        <location>
            <begin position="43"/>
            <end position="200"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002442"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00016463"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU364092"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01578"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ABF10926.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="259" mass="28499" checksum="8CE00C56390B2700" modified="2006-05-30" version="1">MQRDISLDANAHSPRTARARWLAFAAPVRFYPLAGRLAPWCFAVAALFLALGLYLGFVVAPTDAQQGEVYRIIFIHVPAAWMSMFIYVVMAGWCALALVLRTRLSSMMASALAPTGAMFTVIALWTGALWGKPTWGTYWVWDARLTSELILLFLYLGYMALEAAIDEPRRAERACAVLALVGVANVPVIYFSVQWWNTLHQGASVSLTAAPTMAATMLAGMLTMTVACWLYAIGVALVRLRCILRERGEIDTGTLETMP</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="108" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LG41</accession>
    <name>Q1LG41_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Transcriptional regulator, AraC family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">pobR</name>
        <name evidence="4" type="ordered locus">Rmet_4017</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="5">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="5">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10885.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518525.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4017"/>
    <dbReference type="EnsemblBacteria" id="ABF10885">
        <property type="protein sequence ID" value="ABF10885"/>
        <property type="gene ID" value="Rmet_4017"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4017"/>
    <dbReference type="eggNOG" id="COG1917">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG2207">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_88_2_4"/>
    <dbReference type="OMA" id="KHLNRIC"/>
    <dbReference type="OrthoDB" id="1268974at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003313">
        <property type="entry name" value="AraC-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018060">
        <property type="entry name" value="HTH_AraC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011051">
        <property type="entry name" value="RmlC_Cupin_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020449">
        <property type="entry name" value="Tscrpt_reg_HTH_AraC-type"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02311">
        <property type="entry name" value="AraC_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12833">
        <property type="entry name" value="HTH_18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00032">
        <property type="entry name" value="HTHARAC"/>
    </dbReference>
    <dbReference type="SMART" id="SM00342">
        <property type="entry name" value="HTH_ARAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51182">
        <property type="entry name" value="SSF51182"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01124">
        <property type="entry name" value="HTH_ARAC_FAMILY_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0614">Plasmid</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH araC/xylS-type" evidence="3">
        <location>
            <begin position="186"/>
            <end position="284"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS01124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF10885.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="309" mass="34275" checksum="CC9CCE9F0C811511" modified="2006-05-30" version="1">MRTVPTYSLYGVNSSEPLLDQLHFESIASRSQLYAWEIKPHRHERFLQFLYIHRGSGEALLEGRKERLASGSLITVPPHHVHGFVFAPDVDGIIVTMTDTYLRTLLAGLPGTVPLFEHPRHDRVARRHALAGTLALFREEMESVSPWRGASLSALLTLMLVGIARIAEASAPAKTQAGSRPARHFRQFQQLLETDYREQNDIAYYAGAIGVTPTQLNRICRQLAGHSALQLIHARVLAEAQRDLLFSDLDIKQIAMTLGFSDAGYFSRFFARLTGQTPTAFRESGRARLPAFAIKDALNATDMAGAADA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LGD5</accession>
    <name>Q1LGD5_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Multidrug efflux system</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">acrA</name>
        <name evidence="6" type="ordered locus">Rmet_3923</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="7">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="7">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10791.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518439.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3923"/>
    <dbReference type="EnsemblBacteria" id="ABF10791">
        <property type="protein sequence ID" value="ABF10791"/>
        <property type="gene ID" value="Rmet_3923"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3923"/>
    <dbReference type="eggNOG" id="COG0845">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018816_2_1_4"/>
    <dbReference type="OMA" id="INVRYTK"/>
    <dbReference type="OrthoDB" id="1579676at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043602">
        <property type="entry name" value="CusB_dom_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032317">
        <property type="entry name" value="HlyD_D23"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006143">
        <property type="entry name" value="RND_pump_MFP"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00529">
        <property type="entry name" value="CusB_dom_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16576">
        <property type="entry name" value="HlyD_D23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01730">
        <property type="entry name" value="RND_mfp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0614">Plasmid</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193154" evidence="3">
        <location>
            <begin position="25"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="domain" description="HlyD_D23" evidence="5">
        <location>
            <begin position="63"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="domain" description="CusB_dom_1" evidence="4">
        <location>
            <begin position="280"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="365"/>
            <end position="387"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF16576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABF10791.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="387" mass="41034" checksum="E80E0A47EEA3ED65" modified="2006-05-30" version="1">MQAMRARRALIPGILAVAVTVLSACGKDNKPPAAQTPEVKVVTLKDENVTLDSSLPGRTMPYRIAEVRPQVNGILQKRLFVEGSDVKQGQQLYQIDPSTYEATAKSAEANLESARLLAERYGKLVGEEAVSKQQYAEAQASYLQAKAAVDQAHINLRYTKVLSPISGRISRSLVTEGALVTNGQANALATIQQLDPIYVDVTQPSSAILRLRRELANGQLQGAGANAAKVTLQLEDGSIYSEPGRLEFSEVAVDQGTGSVTLRAVFPNPRNELLPGMFVHARLAEGVKTAAILAPQRGVTRDLKGQATSLVVNANNEVEQRILSTDRTVGDKWLVTAGLKAGDRVIVEGLQFVRPGVKVKAAEADAATAGPVEASAPSAASAPAAKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="103" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LGW4</accession>
    <name>Q1LGW4_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Flagellar L-ring protein</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Basal body L-ring protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5 6" type="primary">flgH</name>
        <name evidence="6" type="ordered locus">Rmet_3740</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="7">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="7">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 5">Assembles around the rod to form the L-ring and probably protects the motor/basal body from shearing forces during rotation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">The basal body constitutes a major portion of the flagellar organelle and consists of four rings (L,P,S, and M) mounted on a central rod.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cell outer membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Bacterial flagellum basal body</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the FlgH family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10612.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518258.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3740"/>
    <dbReference type="EnsemblBacteria" id="ABF10612">
        <property type="protein sequence ID" value="ABF10612"/>
        <property type="gene ID" value="Rmet_3740"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3740"/>
    <dbReference type="eggNOG" id="COG2063">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069313_0_0_4"/>
    <dbReference type="OMA" id="ITQQPMT"/>
    <dbReference type="OrthoDB" id="1900876at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009427">
        <property type="term" value="C:bacterial-type flagellum basal body, distal rod, L ring"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009279">
        <property type="term" value="C:cell outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003774">
        <property type="term" value="F:cytoskeletal motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071973">
        <property type="term" value="P:bacterial-type flagellum-dependent cell motility"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00415">
        <property type="entry name" value="FlgH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000527">
        <property type="entry name" value="Flag_Lring"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34933">
        <property type="entry name" value="PTHR34933"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02107">
        <property type="entry name" value="FlgH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01008">
        <property type="entry name" value="FLGLRINGFLGH"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0975">Bacterial flagellum</keyword>
    <keyword evidence="5" id="KW-0998">Cell outer membrane</keyword>
    <keyword evidence="6" id="KW-0966">Cell projection</keyword>
    <keyword evidence="6" id="KW-0969">Cilium</keyword>
    <keyword evidence="6" id="KW-0282">Flagellum</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0614">Plasmid</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002591"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023143"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00415"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABF10612.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="237" mass="24614" checksum="D7FFDAFD993D38AE" modified="2006-05-30" version="1">MTMAATRSGSRIVAFVAHAIVGLAALASGGCAMMPKEPLVQLPTTARAESRPVSAPTGSIFASSYAAGNPLFEDRRPRNVGDILTIVITENINASKNSGSNASRAGNSTLSFNAVPRALAGLFSSASNADIGGTNTLKASGGSSANNTFNGTITVTVLEVLPNGNLVVSGEKQMAINQGAEFIRFSGVVNPRTITGDNGVLSTQVADARIEYTAKGYIDEAQNMGWLQRFFLNVLPF</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LH10</accession>
    <name>Q1LH10_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Response regulator receiver domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">cheY</name>
        <name evidence="3" type="ordered locus">Rmet_3694</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10566.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518215.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3694"/>
    <dbReference type="EnsemblBacteria" id="ABF10566">
        <property type="protein sequence ID" value="ABF10566"/>
        <property type="gene ID" value="Rmet_3694"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3694"/>
    <dbReference type="eggNOG" id="COG0745">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_69_12_4"/>
    <dbReference type="OMA" id="AAGAHEY"/>
    <dbReference type="OrthoDB" id="1630092at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Response regulatory" evidence="2">
        <location>
            <begin position="17"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="1">
        <location>
            <position position="67"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF10566.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="139" mass="15290" checksum="4CEF73D3C99F53DD" modified="2006-05-30" version="1">MAIRNRFGAVTVDKNIKILVVDDFPTMRRIIRNLLKELGFTNVDEAEDGAAGLEKVKDGSFQFVVSDWNMPNMDGLTMLQTIRATPEIAKTPVLMVTAEAKKENIIAAAQAGANGYVVKPFTAATLDEKITKIFEKMGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="62" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LHB0</accession>
    <name>Q1LHB0_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_3594</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10466.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518009.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3594"/>
    <dbReference type="EnsemblBacteria" id="ABF10466">
        <property type="protein sequence ID" value="ABF10466"/>
        <property type="gene ID" value="Rmet_3594"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3594"/>
    <dbReference type="eggNOG" id="ENOG502Z84D">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_070267_0_0_4"/>
    <dbReference type="OMA" id="RYPEYEY"/>
    <dbReference type="OrthoDB" id="911282at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011465">
        <property type="entry name" value="DUF1571"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07608">
        <property type="entry name" value="DUF1571"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193457" evidence="1">
        <location>
            <begin position="19"/>
            <end position="299"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF10466.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="299" mass="33543" checksum="AE39A5D4DE9F58B2" modified="2006-05-30" version="1">MKQLYAAAALGGILGILAAPPGGTTAYAQGTSAPATQTPRVATPYDGLPTSQQATMLSKQVASGELAAQSDAQVLATFEALQPEAILTWARAEMNRYPEYEYSMTRQERLNGQWQDKPARMMIRYRHMPRQLYAKWLPNGVQAGQEIIYDETRRKDEMYGHLGGIMGFTSIWTAIDGTLAKSQSNHTVRDLGFQFVLSMLERDAKTLRAAGLPEKYSRVEIVQEQGVRMVALTWELPEGAPKYYAKKVQLMFDLKHPWPRAETAWDADGNMVEKIVFEKAQKKTFDASTFDPSNSEYKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="105" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LHC0</accession>
    <name>Q1LHC0_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Sensor protein QseC</fullName>
            <ecNumber evidence="4">2.7.13.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">Rmet_3584</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.</text>
            <dbReference type="EC" id="2.7.13.3"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell inner membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.7.13.3" evidence="4"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10456.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517998.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3584"/>
    <dbReference type="EnsemblBacteria" id="ABF10456">
        <property type="protein sequence ID" value="ABF10456"/>
        <property type="gene ID" value="Rmet_3584"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3584"/>
    <dbReference type="eggNOG" id="COG2205">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_89_37_4"/>
    <dbReference type="OMA" id="MARPVYR"/>
    <dbReference type="OrthoDB" id="692375at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00082">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013727">
        <property type="entry name" value="2CSK_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003661">
        <property type="entry name" value="HisK_dim/P"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036097">
        <property type="entry name" value="HisK_dim/P_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004358">
        <property type="entry name" value="Sig_transdc_His_kin-like_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08521">
        <property type="entry name" value="2CSK_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00512">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00344">
        <property type="entry name" value="BCTRLSENSOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00388">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47384">
        <property type="entry name" value="SSF47384"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="SSF55874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="11" id="KW-0418">Kinase</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Histidine kinase" evidence="10">
        <location>
            <begin position="264"/>
            <end position="492"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004429"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00017234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ABF10456.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="495" mass="54253" checksum="C6C1F91215D015D5" modified="2006-05-30" version="1">MWPRRLRQRARLPVRSWLRGSLRRQLLILLVPALALMMAVDTWFTYGTLRDAANTAYDRSLYGSVLTIDNAIGMAGESIQLSLPDAAMEMFETAAQTHVFYRVSMERAGQIETVTGYDDLPLPAGALRNGQPRFYDATYRGDRVRIAAMARPVYRPDAHMRVIIQVAETAEPRAALIGNVWRSALLRDIALIIVSALILVGGITYVLRPLQQVRDDVRARSPEDLTPLEFDHVPVEVRPLVDAVNLHVSRSDAMAQAQAQFIADAAHQLRTPLAILKTQAEFAQRQLDTFGGEGAAHSGAAEAVGGIVKQLEQAARMTNQLLALARVRQHAPDGTAPATEVEIIDAIGVAEQVALDYLPLARGRQQDFGWERPAGLELPVQADPALLREAFANLVHNAIQYSGRRSRITLSATQVDDSAMLVVEDDGPGIPEAEREKVFARFYRRVGHTETGSGLGLAISREMAMRCGGTVVLREGVQGRGVRAELSLPLVMPTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="70" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LHQ1</accession>
    <name>Q1LHQ1_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Rmet_3453</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10325.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517883.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3453"/>
    <dbReference type="EnsemblBacteria" id="ABF10325">
        <property type="protein sequence ID" value="ABF10325"/>
        <property type="gene ID" value="Rmet_3453"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3453"/>
    <dbReference type="eggNOG" id="COG2321">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_059329_0_0_4"/>
    <dbReference type="OMA" id="QKRSQGY"/>
    <dbReference type="OrthoDB" id="1175086at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007343">
        <property type="entry name" value="Uncharacterised_pept_Zn_put"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30168">
        <property type="entry name" value="PTHR30168"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04228">
        <property type="entry name" value="Zn_peptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABF10325.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="297" mass="32410" checksum="BCE3073D50E09EE2" modified="2006-05-30" version="1">MRLDDEAESQNVEDRRGGGYGGGGGFGLPIGGKSIGIGTIVIALAASYFFGIDPSLIFQGASVVQNQQQQAPQSQAHRPPANDQLTVFTRKVLGNTERTWEHIFETDLNRRYAPPKLVLFSGATQTACGTGQSAMGPFYCPGDQKVYIDLSFYDELRQRFGAGGDFAQAYVIAHEIGHHVQNLLGVSEKVDAARRRMNEAQANQLSVRMELQADCLAGVWAANAQKLNQQLLEPGDIEQGLKAAAAIGDDRLQRQAQGYVVPEAFTHGTSEQRVRWLRQGLTTGDIRKCDTFAARQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LHT1</accession>
    <name>Q1LHT1_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Sulfur oxidation protein (SoxY)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">soxY</name>
        <name evidence="3" type="ordered locus">Rmet_3423</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10295.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517858.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3423"/>
    <dbReference type="EnsemblBacteria" id="ABF10295">
        <property type="protein sequence ID" value="ABF10295"/>
        <property type="gene ID" value="Rmet_3423"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3423"/>
    <dbReference type="eggNOG" id="COG5501">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_118521_0_0_4"/>
    <dbReference type="OMA" id="RVKMGQT"/>
    <dbReference type="OrthoDB" id="1747553at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.2470">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032711">
        <property type="entry name" value="SoxY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038162">
        <property type="entry name" value="SoxY_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016568">
        <property type="entry name" value="Sulphur_oxidation_SoxY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006311">
        <property type="entry name" value="TAT_signal"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13501">
        <property type="entry name" value="SoxY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF010312">
        <property type="entry name" value="Sulphur_oxidation_SoxY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR04488">
        <property type="entry name" value="SoxY_true_GGCGG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51318">
        <property type="entry name" value="TAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193478" evidence="1">
        <location>
            <begin position="30"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="domain" description="SoxY" evidence="2">
        <location>
            <begin position="49"/>
            <end position="149"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF10295.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="151" mass="15559" checksum="27D689C73999350A" modified="2006-05-30" version="1">MNAKRRDVLRMTAVLSLMAATGLISEAQAAEWNKTAFDGKSVADVIKALGGSGTEKSSAITFIAPDIAENGAVVPVAVTSNIPDTEQIAILVEKNPNTLAADFTIPAGTEPFVSTRVKMGQTSVVHAAVKAGGKWYVASKEIKVTLGGCGG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2007-11-13" modified="2022-05-25" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LI40</accession>
    <name>RL2_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">50S ribosomal protein L2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rplB</name>
        <name type="ordered locus">Rmet_3314</name>
    </gene>
    <organism>
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">One of the primary rRNA binding proteins. Required for association of the 30S and 50S subunits to form the 70S ribosome, for tRNA binding and peptide bond formation. It has been suggested to have peptidyltransferase activity; this is somewhat controversial. Makes several contacts with the 16S rRNA in the 70S ribosome.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the 50S ribosomal subunit. Forms a bridge to the 30S subunit in the 70S ribosome.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL2 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10186.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008642926.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q1LI40"/>
    <dbReference type="SMR" id="Q1LI40"/>
    <dbReference type="STRING" id="266264.Rmet_3314"/>
    <dbReference type="EnsemblBacteria" id="ABF10186">
        <property type="protein sequence ID" value="ABF10186"/>
        <property type="gene ID" value="Rmet_3314"/>
    </dbReference>
    <dbReference type="GeneID" id="60826603"/>
    <dbReference type="KEGG" id="rme:Rmet_3314"/>
    <dbReference type="eggNOG" id="COG0090">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036235_2_1_4"/>
    <dbReference type="OMA" id="SCIELRP"/>
    <dbReference type="OrthoDB" id="961486at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015934">
        <property type="term" value="C:large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.950.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01320_B">
        <property type="entry name" value="Ribosomal_L2_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022666">
        <property type="entry name" value="Rbsml_prot_L2_RNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014722">
        <property type="entry name" value="Rib_L2_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002171">
        <property type="entry name" value="Ribosomal_L2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005880">
        <property type="entry name" value="Ribosomal_L2_bac/org-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022669">
        <property type="entry name" value="Ribosomal_L2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022671">
        <property type="entry name" value="Ribosomal_L2_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014726">
        <property type="entry name" value="Ribosomal_L2_dom3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008991">
        <property type="entry name" value="Translation_prot_SH3-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13691">
        <property type="entry name" value="PTHR13691"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00181">
        <property type="entry name" value="Ribosomal_L2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03947">
        <property type="entry name" value="Ribosomal_L2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002158">
        <property type="entry name" value="Ribosomal_L2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01383">
        <property type="entry name" value="Ribosomal_L2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01382">
        <property type="entry name" value="Ribosomal_L2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50104">
        <property type="entry name" value="SSF50104"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01171">
        <property type="entry name" value="rplB_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00467">
        <property type="entry name" value="RIBOSOMAL_L2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <feature type="chain" id="PRO_0000309993" description="50S ribosomal protein L2">
        <location>
            <begin position="1"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="38"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="225"/>
            <end position="276"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="276" mass="30076" checksum="A4F441BA1FD2E3D4" modified="2006-05-30" version="1">MALVKTKPTSPGRRSMVKVVNKDLHKGAPHAPLLEKQFQKSGRNNNGHITTRHKGGGHKHHYRVVDFKRNDKDGIAAKVERLEYDPNRSANIALVLFADGERRYIIATKGMVAGQALMNGSEAPIKAGNNLPIRNIPVGTTINNVEILPGKGAQVARAAGGSAVLLAREGLYAQVRLRSGEVRRVHIECRATIGEVGNEEHSLRVIGKAGATRWRGIRPTVRGVVMNPVDHPHGGGEGKTAAGRDPVSPWGTPAKGYRTRSNKRTDSMIVQRRHKR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2007-01-23" modified="2022-05-25" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LI55</accession>
    <name>RL30_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">50S ribosomal protein L30</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpmD</name>
        <name type="ordered locus">Rmet_3299</name>
    </gene>
    <organism>
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="1">Part of the 50S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL30 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10171.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q1LI55"/>
    <dbReference type="SMR" id="Q1LI55"/>
    <dbReference type="STRING" id="266264.Rmet_3299"/>
    <dbReference type="EnsemblBacteria" id="ABF10171">
        <property type="protein sequence ID" value="ABF10171"/>
        <property type="gene ID" value="Rmet_3299"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3299"/>
    <dbReference type="eggNOG" id="COG1841">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_131047_1_4_4"/>
    <dbReference type="OMA" id="GKQNQRD"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015934">
        <property type="term" value="C:large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01658">
        <property type="entry name" value="Ribosomal_L30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1390.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01371_B">
        <property type="entry name" value="Ribosomal_L30_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036919">
        <property type="entry name" value="L30_ferredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005996">
        <property type="entry name" value="Ribosomal_L30_bac-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016082">
        <property type="entry name" value="Ribosomal_L30_ferredoxin-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15892">
        <property type="entry name" value="PTHR15892"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00327">
        <property type="entry name" value="Ribosomal_L30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002211">
        <property type="entry name" value="Ribosomal_L30_bac-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55129">
        <property type="entry name" value="SSF55129"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01308">
        <property type="entry name" value="rpmD_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <feature type="chain" id="PRO_0000273837" description="50S ribosomal protein L30">
        <location>
            <begin position="1"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01371"/>
        </source>
    </evidence>
    <sequence length="60" mass="6690" checksum="F2FD6E5A7E38B701" modified="2006-05-30" version="1">MSQKTVKVQLVRSLIGTREDHRATVRGLGLRRLNSVSELQDTPAVRGMINKVSYLVKVLA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-12-12" modified="2022-05-25" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LI58</accession>
    <name>IF12_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Translation initiation factor IF-1 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">infA2</name>
        <name type="ordered locus">Rmet_3296</name>
    </gene>
    <organism>
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">One of the essential components for the initiation of protein synthesis. Stabilizes the binding of IF-2 and IF-3 on the 30S subunit to which N-formylmethionyl-tRNA(fMet) subsequently binds. Helps modulate mRNA selection, yielding the 30S pre-initiation complex (PIC). Upon addition of the 50S ribosomal subunit IF-1, IF-2 and IF-3 are released leaving the mature 70S translation initiation complex.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Component of the 30S ribosomal translation pre-initiation complex which assembles on the 30S ribosome in the order IF-2 and IF-3, IF-1 and N-formylmethionyl-tRNA(fMet); mRNA recruitment can occur at any time during PIC assembly.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the IF-1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10168.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008642956.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q1LI58"/>
    <dbReference type="SMR" id="Q1LI58"/>
    <dbReference type="STRING" id="266264.Rmet_3296"/>
    <dbReference type="EnsemblBacteria" id="ABF10168">
        <property type="protein sequence ID" value="ABF10168"/>
        <property type="gene ID" value="Rmet_3296"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3296"/>
    <dbReference type="eggNOG" id="COG0361">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_151267_1_0_4"/>
    <dbReference type="OMA" id="ECLRSAM"/>
    <dbReference type="OrthoDB" id="2066663at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043022">
        <property type="term" value="F:ribosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd04451">
        <property type="entry name" value="S1_IF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00075">
        <property type="entry name" value="IF_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006196">
        <property type="entry name" value="RNA-binding_domain_S1_IF1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022967">
        <property type="entry name" value="S1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004368">
        <property type="entry name" value="TIF_IF1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33370">
        <property type="entry name" value="PTHR33370"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01176">
        <property type="entry name" value="eIF-1a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00316">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00008">
        <property type="entry name" value="infA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50832">
        <property type="entry name" value="S1_IF1_TYPE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0396">Initiation factor</keyword>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <feature type="chain" id="PRO_0000263850" description="Translation initiation factor IF-1 2">
        <location>
            <begin position="1"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="domain" description="S1-like" evidence="1">
        <location>
            <begin position="1"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00075"/>
        </source>
    </evidence>
    <sequence length="72" mass="8239" checksum="983F14C587BB0CBF" modified="2006-05-30" version="1">MAKDDVIQMQGEVLENLPNATFRVKLENGHIVLGHISGKMRMNYIRILPGDKVTVELTPYDLSRARIVFRTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="103" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LIF1</accession>
    <name>Q1LIF1_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Release factor glutamine methyltransferase</fullName>
            <shortName evidence="6">RF MTase</shortName>
            <ecNumber evidence="6">2.1.1.297</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">N5-glutamine methyltransferase PrmC</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">Protein-(glutamine-N5) MTase PrmC</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">Protein-glutamine N-methyltransferase PrmC</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6 9" type="primary">prmC</name>
        <name evidence="9" type="ordered locus">Rmet_3203</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2 6">Methylates the class 1 translation termination release factors RF1/PrfA and RF2/PrfB on the glutamine residue of the universally conserved GGQ motif.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 6">
            <text>L-glutaminyl-[peptide chain release factor] + S-adenosyl-L-methionine = H(+) + N(5)-methyl-L-glutaminyl-[peptide chain release factor] + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:42896"/>
            <dbReference type="Rhea" id="RHEA-COMP:10271"/>
            <dbReference type="Rhea" id="RHEA-COMP:10272"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30011"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:61891"/>
            <dbReference type="EC" id="2.1.1.297"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the protein N5-glutamine methyltransferase family. PrmC subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.297" evidence="6"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10075.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3203"/>
    <dbReference type="EnsemblBacteria" id="ABF10075">
        <property type="protein sequence ID" value="ABF10075"/>
        <property type="gene ID" value="Rmet_3203"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3203"/>
    <dbReference type="eggNOG" id="COG2890">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018398_3_1_4"/>
    <dbReference type="OMA" id="FDARYWE"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102559">
        <property type="term" value="F:protein-(glutamine-N5) methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036009">
        <property type="term" value="F:protein-glutamine N-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018364">
        <property type="term" value="P:peptidyl-glutamine methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02126">
        <property type="entry name" value="RF_methyltr_PrmC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002052">
        <property type="entry name" value="DNA_methylase_N6_adenine_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004556">
        <property type="entry name" value="HemK-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040758">
        <property type="entry name" value="PrmC_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019874">
        <property type="entry name" value="Release_fac_Glu-N5_MeTfrase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007848">
        <property type="entry name" value="Small_mtfrase_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05175">
        <property type="entry name" value="MTS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17827">
        <property type="entry name" value="PrmC_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00536">
        <property type="entry name" value="hemK_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03534">
        <property type="entry name" value="RF_mod_PrmC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00092">
        <property type="entry name" value="N6_MTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6 9" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 6" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="4 6 9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PrmC_N" evidence="8">
        <location>
            <begin position="48"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="domain" description="MTS" evidence="7">
        <location>
            <begin position="136"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="region of interest" description="S-adenosyl-L-methionine binding" evidence="6">
        <location>
            <begin position="149"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="6">
        <location>
            <begin position="216"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="172"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="200"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="216"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF05175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF17827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ABF10075.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="310" mass="33577" checksum="296F2C7DCDD447B0" modified="2006-05-30" version="1">MFRRGFLQTGVPVPQMVEVSSKPMSPSPQGALPPAPTLREALTLAAIAGLPVLEARMLLSHVTGFTRTQLITRDNDHLDAPKRDAFATLLARRLTGEPMAYLIGEREFFGRTFRVTPDVLIPRPDTEVAAEASLARIADVKAPRVLDMGTGSGILAVTIARERPDAEVWATDISRGALMVAEDNARALQAENIRFLVSDWYEDLPVGLCFNLIVSNPPYIAEGDPHLVEGDLRFEPIDALTDHEDGLSDLATIVAGATARLLPGGWLLMEHGYDQGAAMRAQLAEAGFVEVFTTRDLAGLERCSGGRLRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="72" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LIZ3</accession>
    <name>Q1LIZ3_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Transposase (Tn6048)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">tniA</name>
        <name evidence="3" type="ordered locus">Rmet_3010</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09883.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3010"/>
    <dbReference type="EnsemblBacteria" id="ABF09883">
        <property type="protein sequence ID" value="ABF09883"/>
        <property type="gene ID" value="Rmet_3010"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3010"/>
    <dbReference type="HOGENOM" id="CLU_017991_4_0_4"/>
    <dbReference type="OMA" id="PEYGGHI"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001584">
        <property type="entry name" value="Integrase_cat-core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015378">
        <property type="entry name" value="Transposase-like_Mu_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09299">
        <property type="entry name" value="Mu-transpos_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00665">
        <property type="entry name" value="rve"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="SSF53098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50994">
        <property type="entry name" value="INTEGRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Integrase catalytic" evidence="2">
        <location>
            <begin position="121"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="457"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="457"/>
            <end position="474"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF09883.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="509" mass="57392" checksum="31862D07462A8278" modified="2006-05-30" version="1">MGLSRRQVYVLIRRARLGSGLVTDLALGQSSGGKGKGRLPESVERIIRELLQKRFLTKQKRSLAAFHREVVRACKLQKLRVPARNTVALRIAGLDPREVTHRREGQDAARDLQGVGGVPPPVSAPLEQVQIDHTVIDLIVVDERDRQPIGRPYLTLAIDVFTRCVVGMVVTLEAPSAVSVGLCLVHAACDKRPWLEGLNVEMDWPMSGKPRLLYLDNAAEFKSEALRRGCEQHGIRLDYRPLGQPHYGGIVERIIGTAMQMIHDELPGTTFSNPDQRGEYASEKMAALTLRELERWLTLAVGTYHGSVHNGLLQPPAARWAEAITRTGVPTVITRATAFLVDFLPIIRRTLTRTGFVIDHIHYYADALKPWIARRDRLPAFLIRRDPRDISRIWVLEPEGQHYLEIPYRTLSHPAVTLWEQRQALAKLRQQGREQVDESALFRMIGQMREIVTTAQKATRKARRDADRRQHLKATATPVKTTPPPGADMDGQQADNQPPAKPFDQIEEW</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-04-07" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LJ28</accession>
    <name>Q1LJ28_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Outer membrane protein assembly factor BamE</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">omlA</name>
        <name evidence="4" type="synonym">bamE</name>
        <name evidence="7" type="ordered locus">Rmet_2975</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Part of the outer membrane protein assembly complex, which is involved in assembly and insertion of beta-barrel proteins into the outer membrane.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Part of the Bam complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cell outer membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the BamE family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09848.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2975"/>
    <dbReference type="EnsemblBacteria" id="ABF09848">
        <property type="protein sequence ID" value="ABF09848"/>
        <property type="gene ID" value="Rmet_2975"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2975"/>
    <dbReference type="eggNOG" id="COG2913">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_083835_1_0_4"/>
    <dbReference type="OMA" id="WDYVFYF"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009279">
        <property type="term" value="C:cell outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043165">
        <property type="term" value="P:Gram-negative-bacterium-type cell outer membrane assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051205">
        <property type="term" value="P:protein insertion into membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1450.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00925">
        <property type="entry name" value="OM_assembly_BamE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026592">
        <property type="entry name" value="BamE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037873">
        <property type="entry name" value="BamE-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007450">
        <property type="entry name" value="Lipoprotein_SmpA/OmlA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37482">
        <property type="entry name" value="PTHR37482"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04355">
        <property type="entry name" value="SmpA_OmlA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0998">Cell outer membrane</keyword>
    <keyword evidence="7" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="2 4" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0732">Signal</keyword>
    <feature type="domain" description="SmpA_OmlA" evidence="6">
        <location>
            <begin position="92"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="227"/>
            <end position="258"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00925"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF04355"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABF09848.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="258" mass="27489" checksum="650B530C68C18D3A" modified="2006-05-30" version="1">MTRTRRSPRSDQNAPGRAGCLRSLWGVPVPVSTHSRLEARYMRSFRSSAMRPALVSSLLAAALLAGACSAYDSTSRKVADAITPYRINIVQGNFVSREAVSQLREGMTRDQVRFLLGTPLLTDVFHADRWDYYFSFRRGSRPVVQSRQFTVYFEGDKLVRWVGNELPSEYELIAEIDGMKAAQKNQTPGKIVFGGEPAPASAPAAMPASAPAAMPVAAASEPAAAPAAVAPAAAEAAQQKPAEAPASAASDVPAKPAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="72" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LJK7</accession>
    <name>Q1LJK7_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Extra-cytoplasmic Solute Receptor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">bug</name>
        <name evidence="4" type="ordered locus">Rmet_2796</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Periplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the UPF0065 (bug) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09669.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517364.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2796"/>
    <dbReference type="EnsemblBacteria" id="ABF09669">
        <property type="protein sequence ID" value="ABF09669"/>
        <property type="gene ID" value="Rmet_2796"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2796"/>
    <dbReference type="eggNOG" id="COG3181">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045683_0_0_4"/>
    <dbReference type="OMA" id="FMGAVHH"/>
    <dbReference type="OrthoDB" id="447734at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042597">
        <property type="term" value="C:periplasmic space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005064">
        <property type="entry name" value="BUG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042100">
        <property type="entry name" value="Bug_dom1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42928">
        <property type="entry name" value="PTHR42928"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03401">
        <property type="entry name" value="TctC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017082">
        <property type="entry name" value="YflP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0675">Receptor</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193163" evidence="3">
        <location>
            <begin position="34"/>
            <end position="336"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF09669.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="336" mass="35756" checksum="9AD653A1CF2F975A" modified="2006-05-30" version="1">MNRRQRNLTTFARVFAVAGLMAGTGVTSLYAPAAQADTWPSKPVTVIVPFPAGGGTDAFARPLTAQLSKQLGKQFVIDNRGGAGGTVGASIAAKAAPDGYTLFIGGAHHAIAPSFYKKLDYDIEKDFIPITVIAQPPQVIVVNSGKVQATSVKDLIAYAKANPGKLNYASAGNGSSHHLAGELFKLQTQTDLIHVPYKGAGPALSDLIAGQVDLMFDGLGSSSQHIRAGRIKALAVASKTRSAAFPNVPTAAEAGLPNYDVSTWYALWAPKGTPKEVVDRLYAETTKALNNPEMKQIWLQNGSDIPQYTPEQFAQFQHAEIKRWADVVQRSGAKID</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="67" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LJL0</accession>
    <name>Q1LJL0_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative periplasmic or secreted protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_2793</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09666.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517361.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2793"/>
    <dbReference type="EnsemblBacteria" id="ABF09666">
        <property type="protein sequence ID" value="ABF09666"/>
        <property type="gene ID" value="Rmet_2793"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2793"/>
    <dbReference type="eggNOG" id="COG3471">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_086898_0_0_4"/>
    <dbReference type="OMA" id="YPRYGKD"/>
    <dbReference type="OrthoDB" id="1825422at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007497">
        <property type="entry name" value="DUF541"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04402">
        <property type="entry name" value="SIMPL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193221" evidence="1">
        <location>
            <begin position="18"/>
            <end position="229"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF09666.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="229" mass="23878" checksum="BB2CEAE6624EB1D1" modified="2006-05-30" version="1">MKKLLAASLLSTTAAMAAAQTVAPPSGVLSLSAQAATEVPTDVVHLTLAAEQEGPEPTAISNALSSRTQAVLAQAKRTSGVEAQSGGFTIHPNTDRNGRISTWRGRSEVILKSKDFAAVSKLAGELASQMQVQNIAFSLSRETRLAAEQKLADQAVAAFRDKAQATTKLFGYSGYTIREVSLNESGGVMPMPRMYVAKAMSDSAGAPIPVEGGKSQVTVSVNGSVQMVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LJM3</accession>
    <name>Q1LJM3_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Cobalamin biosynthesis protein CobD</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10 11" type="primary">cobD</name>
        <name evidence="11" type="ordered locus">Rmet_2780</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Converts cobyric acid to cobinamide by the addition of aminopropanol on the F carboxylic group.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3 10">Cofactor biosynthesis; adenosylcobalamin biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 10">Cell membrane</location>
            <topology evidence="2 10">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 10">Belongs to the CobD/CbiB family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09653.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517351.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2780"/>
    <dbReference type="EnsemblBacteria" id="ABF09653">
        <property type="protein sequence ID" value="ABF09653"/>
        <property type="gene ID" value="Rmet_2780"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2780"/>
    <dbReference type="eggNOG" id="COG1270">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_054212_1_0_4"/>
    <dbReference type="OMA" id="WGYRNER"/>
    <dbReference type="OrthoDB" id="2029688at2"/>
    <dbReference type="UniPathway" id="UPA00148"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015420">
        <property type="term" value="F:ABC-type vitamin B12 transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016874">
        <property type="term" value="F:ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048472">
        <property type="term" value="F:threonine-phosphate decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009236">
        <property type="term" value="P:cobalamin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00024">
        <property type="entry name" value="CobD_CbiB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004485">
        <property type="entry name" value="Cobalamin_biosynth_CobD/CbiB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34308">
        <property type="entry name" value="PTHR34308"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03186">
        <property type="entry name" value="CobD_Cbib"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00380">
        <property type="entry name" value="cobD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 10" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 10" id="KW-0169">Cobalamin biosynthesis</keyword>
    <keyword evidence="11" id="KW-0436">Ligase</keyword>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="58"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="162"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="301"/>
            <end position="322"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022573"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ABF09653.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="326" mass="34714" checksum="E54CC47FED19E109" modified="2006-05-30" version="1">MIMLSWPACVAAAIAGVLLDRWLGEPRRWHPLVGFGRLATAVANRLNRPERGALRQRVAGLIAWAVVVLVPAAIAVWLISLAQAVHPALAWVLHAVALYAALGARSLSQHIAPIAGALTRGDLYAARRLTACIVSRDTEDADAEALARAACESALENGNDAIFGALFWFLIGGAPAVIVFRLANTLDAMWGYRTPRWLMFGWAAARIDDVLNFVPARLTALSYASLGATAQALRCWRTQARAWSSPNAGPVMAAGAGAVGVALGGGARYHGEWEERPPLGAGDAPAAIHIRACLRLVQRAVWLWLGLAMLSVPLVDLLAGLLERTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="95" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LJN9</accession>
    <name>Q1LJN9_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">4Fe-4S ferredoxin, iron-sulfur binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Rmet_2764</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09637.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517335.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2764"/>
    <dbReference type="EnsemblBacteria" id="ABF09637">
        <property type="protein sequence ID" value="ABF09637"/>
        <property type="gene ID" value="Rmet_2764"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2764"/>
    <dbReference type="eggNOG" id="COG1148">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022482_0_0_4"/>
    <dbReference type="OMA" id="CTLCMAC"/>
    <dbReference type="OrthoDB" id="1619561at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00037">
        <property type="entry name" value="Fer4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12838">
        <property type="entry name" value="Fer4_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13187">
        <property type="entry name" value="Fer4_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0408">Iron</keyword>
    <keyword evidence="3" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="4">
        <location>
            <begin position="213"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="4">
        <location>
            <begin position="366"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="4">
        <location>
            <begin position="586"/>
            <end position="611"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="4">
        <location>
            <begin position="615"/>
            <end position="646"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABF09637.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="726" mass="76106" checksum="62A5A4A6A7D15638" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-10-31" modified="2022-05-25" version="66" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LJR0</accession>
    <name>Y2743_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">UPF0301 protein Rmet_2743</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">Rmet_2743</name>
    </gene>
    <organism>
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0301 (AlgH) family.</text>
    </comment>
    <comment type="sequence caution" evidence="2">
        <conflict type="erroneous initiation">
            <sequence resource="EMBL-CDS" id="ABF09616" version="1"/>
        </conflict>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09616.1"/>
        <property type="status" value="ALT_INIT"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008646285.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q1LJR0"/>
    <dbReference type="SMR" id="Q1LJR0"/>
    <dbReference type="STRING" id="266264.Rmet_2743"/>
    <dbReference type="EnsemblBacteria" id="ABF09616">
        <property type="protein sequence ID" value="ABF09616"/>
        <property type="gene ID" value="Rmet_2743"/>
    </dbReference>
    <dbReference type="GeneID" id="60820806"/>
    <dbReference type="KEGG" id="rme:Rmet_2743"/>
    <dbReference type="eggNOG" id="COG1678">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_057596_1_0_4"/>
    <dbReference type="OrthoDB" id="1736059at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00758">
        <property type="entry name" value="UPF0301"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003774">
        <property type="entry name" value="UPF0301"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30327">
        <property type="entry name" value="PTHR30327"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02622">
        <property type="entry name" value="DUF179"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000258863" description="UPF0301 protein Rmet_2743">
        <location>
            <begin position="1"/>
            <end position="190"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00758"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="190" mass="20329" checksum="8AF16604FFABA64D" modified="2006-10-31" version="2">MATSEAPINLTNQFLIAMPGMADPTFSGSVVYLCEHNERGALGLVINRPIDIDMATLFDKIDLKLEIQPVAHQPVYFGGPVQTERGFVLHDPVGIYVSSLAVPGGLEMTTSKDVLEAVANGSGPHRFLLTLGYAGWGAGQLEDELSRNGWLTVQADPEIIFSVPPEERFAAAIRLLGIDISMLSGDAGHA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="57" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LJW3</accession>
    <name>Q1LJW3_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_2690</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09563.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517263.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2690"/>
    <dbReference type="EnsemblBacteria" id="ABF09563">
        <property type="protein sequence ID" value="ABF09563"/>
        <property type="gene ID" value="Rmet_2690"/>
    </dbReference>
    <dbReference type="GeneID" id="60820872"/>
    <dbReference type="KEGG" id="rme:Rmet_2690"/>
    <dbReference type="HOGENOM" id="CLU_2878339_0_0_4"/>
    <dbReference type="OMA" id="HLGQNID"/>
    <dbReference type="OrthoDB" id="2083043at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025906">
        <property type="entry name" value="YjfB_motility"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14070">
        <property type="entry name" value="YjfB_motility"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="44"/>
            <end position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF09563.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="63" mass="6456" checksum="4EF61541E6C4475E" modified="2006-05-30" version="1">MINSIVSSLNASDPGSDPVGLTMLRKTLDLQSQGVAQLLQSMPGVGSAGSNPPHLGQNIDIKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="64" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LK54</accession>
    <name>Q1LK54_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_2595</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09472.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517175.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2595"/>
    <dbReference type="EnsemblBacteria" id="ABF09472">
        <property type="protein sequence ID" value="ABF09472"/>
        <property type="gene ID" value="Rmet_2595"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2595"/>
    <dbReference type="eggNOG" id="ENOG5033K93">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_180639_0_0_4"/>
    <dbReference type="OMA" id="NSQCRQD"/>
    <dbReference type="OrthoDB" id="2025185at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193173" evidence="2">
        <location>
            <begin position="27"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="26"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="33"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="64"/>
            <end position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF09472.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="85" mass="9515" checksum="18AFC55F033D25BC" modified="2006-05-30" version="1">MTNICRKLGALVIAMVVIPAPGLAGATEQAQQRRAGRDVRQDTRQGSRDTKQDCRAANQKSNSQCRQDKRDTKQQGRQTARDIKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="64" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LK72</accession>
    <name>Q1LK72_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">RanBP2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_2577</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09454.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517157.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2577"/>
    <dbReference type="EnsemblBacteria" id="ABF09454">
        <property type="protein sequence ID" value="ABF09454"/>
        <property type="gene ID" value="Rmet_2577"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2577"/>
    <dbReference type="eggNOG" id="ENOG5032Y5P">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_155686_0_0_4"/>
    <dbReference type="OMA" id="CELHNRY"/>
    <dbReference type="OrthoDB" id="1917736at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018551">
        <property type="entry name" value="DUF2007"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001876">
        <property type="entry name" value="Znf_RanBP2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09413">
        <property type="entry name" value="DUF2007"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50199">
        <property type="entry name" value="ZF_RANBP2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RanBP2-type" evidence="2">
        <location>
            <begin position="72"/>
            <end position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF09454.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="105" mass="11431" checksum="D9B8508A82B26C71" modified="2006-05-30" version="1">MKLLLRTPSLVHAAHCENVLRAAGIQAEVRNTWLAGAAGDIPLQESAPQVWILDDSTEADAWAVLNAAANPRPGPHWQCRHCGEWHEAQFAACWRCGQPQDQAAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="64" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LKV9</accession>
    <name>Q1LKV9_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Rmet_2340</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09217.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_003153640.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2340"/>
    <dbReference type="EnsemblBacteria" id="ABF09217">
        <property type="protein sequence ID" value="ABF09217"/>
        <property type="gene ID" value="Rmet_2340"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2340"/>
    <dbReference type="eggNOG" id="COG1746">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_051351_0_1_4"/>
    <dbReference type="OMA" id="ANSAWKR"/>
    <dbReference type="OrthoDB" id="1258110at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016779">
        <property type="term" value="F:nucleotidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008152">
        <property type="term" value="P:metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05400">
        <property type="entry name" value="NT_2-5OAS_ClassI-CCAase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.460.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006116">
        <property type="entry name" value="NT_2-5OAS_ClassI-CCAase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043519">
        <property type="entry name" value="NT_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81301">
        <property type="entry name" value="SSF81301"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="1" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF09217.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="300" mass="33997" checksum="E97D87EDA6241B7B" modified="2006-05-30" version="1">MLSIDEAFRKFKSRLELNEREQKNASQRQNEVRDYLQTKFGIARSFLTGSYARYTKTKPLKDIDIFFVLKDSEKHYHGKAASVVLDDFHSALVEKYGSAAVRKQARSINVDFGVHIDAEDNTDYRVVSVDAVPAFDTGDQYEIPDTASGKWIKTDPEIHKDKATAAHQAYGNEWKGLVRMVKYWNNNPKHGDLKPVKPSFLIEVMALECLYGGWGGSFDREIQSFFATLADRVHDEWPDPAGLGPAISNDMDAARKQRAQQLLFQASQDASIAIDHARRGRNIEALRAWRALFGPKFPLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="57" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LL15</accession>
    <name>Q1LL15_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_2282</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09161.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516986.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2282"/>
    <dbReference type="EnsemblBacteria" id="ABF09161">
        <property type="protein sequence ID" value="ABF09161"/>
        <property type="gene ID" value="Rmet_2282"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2282"/>
    <dbReference type="HOGENOM" id="CLU_129162_1_1_4"/>
    <dbReference type="OMA" id="HGYGGWG"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193256" evidence="1">
        <location>
            <begin position="23"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF09161.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="108" mass="11431" checksum="4A9467F3130A1B3F" modified="2006-05-30" version="1">MKRWLFAAFGVTAALMSGAAMAHVSVGVAIGVPGVVIGAPAYYPPAPVYYAPPPVVVAPGPAYYGPPPVVVRPAPVYYGGYGYYRGGPRYYYGPGWHGRGHGHGRWHD</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="64" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LL55</accession>
    <name>Q1LL55_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_2242</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09121.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516946.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2242"/>
    <dbReference type="EnsemblBacteria" id="ABF09121">
        <property type="protein sequence ID" value="ABF09121"/>
        <property type="gene ID" value="Rmet_2242"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2242"/>
    <dbReference type="eggNOG" id="ENOG5033IE5">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_139747_0_0_4"/>
    <dbReference type="OMA" id="AYWAGAS"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024447">
        <property type="entry name" value="YXWGXW_rpt"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12779">
        <property type="entry name" value="WXXGXW"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193187" evidence="2">
        <location>
            <begin position="29"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="103"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="108"/>
            <end position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF09121.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="143" mass="15750" checksum="947DFF4218801781" modified="2006-05-30" version="1">MKRQLIVRAFAGAVLLGSAAALAPAAMAQVSINIGIGVPPPAPVYEVVPPPRAGYVWAPGYWDWDDHGHKHAWKKGHWVGERPGYVYEQPRWVRASNGWVLQPERWNRGPGRGDDDDQGHGHDHDRGRGGYHCPPGHAKKGEC</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LLM0</accession>
    <name>Q1LLM0_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Nitrate reductase 1, gamma (Cytochrome b(NR)) subunit</fullName>
            <ecNumber evidence="15">1.7.99.4</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">narI</name>
        <name evidence="15" type="ordered locus">Rmet_2077</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="1.7.99.4" evidence="15"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08956.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516790.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2077"/>
    <dbReference type="EnsemblBacteria" id="ABF08956">
        <property type="protein sequence ID" value="ABF08956"/>
        <property type="gene ID" value="Rmet_2077"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2077"/>
    <dbReference type="eggNOG" id="COG2181">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_092378_1_0_4"/>
    <dbReference type="OMA" id="MVSGGFF"/>
    <dbReference type="OrthoDB" id="1873056at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009325">
        <property type="term" value="C:nitrate reductase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008940">
        <property type="term" value="F:nitrate reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042128">
        <property type="term" value="P:nitrate assimilation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023234">
        <property type="entry name" value="NarG-like_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036197">
        <property type="entry name" value="NarG-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003816">
        <property type="entry name" value="Nitrate_red_gam"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02665">
        <property type="entry name" value="Nitrate_red_gam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103501">
        <property type="entry name" value="SSF103501"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00351">
        <property type="entry name" value="narI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0249">Electron transport</keyword>
    <keyword evidence="4" id="KW-0349">Heme</keyword>
    <keyword evidence="10" id="KW-0408">Iron</keyword>
    <keyword evidence="12 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0534">Nitrate assimilation</keyword>
    <keyword evidence="9 15" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 13" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="53"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="89"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="130"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="189"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="domain" description="Nitrate_red_gam" evidence="14">
        <location>
            <begin position="5"/>
            <end position="227"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF02665"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="ABF08956.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="227" mass="25271" checksum="3C7AAF2CD5381BFE" modified="2006-05-30" version="1">MNLVHQFIFGIYPYIAAAIFLFGSLARFEREQYTWKSDSSQMLYRGNLRTGNILFHVGILGLFFGHLVGLLTPVVVWDTLGVSHGLKQAIAMSAGGVMGTMCLAGLLILLHRRLTNARVSAVTRFGDKVLLLWLLVTLLLGLSTIFESASHMDGHMMVQLMTWAQHVVTLRADAASYVAEAPLLFKAHLFMGISLFAIFPFTRLVHVWSGFASVTYLGRAWQLVRGR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-01-15" modified="2022-05-25" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LLX0</accession>
    <name>RS18_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">30S ribosomal protein S18</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpsR</name>
        <name type="ordered locus">Rmet_1977</name>
    </gene>
    <organism>
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Binds as a heterodimer with protein S6 to the central domain of the 16S rRNA, where it helps stabilize the platform of the 30S subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the 30S ribosomal subunit. Forms a tight heterodimer with protein S6.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the bacterial ribosomal protein bS18 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08856.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_006163606.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q1LLX0"/>
    <dbReference type="SMR" id="Q1LLX0"/>
    <dbReference type="STRING" id="266264.Rmet_1977"/>
    <dbReference type="EnsemblBacteria" id="ABF08856">
        <property type="protein sequence ID" value="ABF08856"/>
        <property type="gene ID" value="Rmet_1977"/>
    </dbReference>
    <dbReference type="GeneID" id="60821417"/>
    <dbReference type="KEGG" id="rme:Rmet_1977"/>
    <dbReference type="eggNOG" id="COG0238">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_148710_0_3_4"/>
    <dbReference type="OMA" id="QKKYCRF"/>
    <dbReference type="OrthoDB" id="1940575at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.640.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00270">
        <property type="entry name" value="Ribosomal_S18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001648">
        <property type="entry name" value="Ribosomal_S18"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018275">
        <property type="entry name" value="Ribosomal_S18_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036870">
        <property type="entry name" value="Ribosomal_S18_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13479">
        <property type="entry name" value="PTHR13479"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01084">
        <property type="entry name" value="Ribosomal_S18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00974">
        <property type="entry name" value="RIBOSOMALS18"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46911">
        <property type="entry name" value="SSF46911"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00165">
        <property type="entry name" value="S18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00057">
        <property type="entry name" value="RIBOSOMAL_S18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <feature type="chain" id="PRO_1000003580" description="30S ribosomal protein S18">
        <location>
            <begin position="1"/>
            <end position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00270"/>
        </source>
    </evidence>
    <sequence length="92" mass="10891" checksum="EEF2166705A2F38B" modified="2006-05-30" version="1">MAFVKRDNKNKKRFQQQNPLFKRKRFCRFTVAGVEQIDYKDLDTLKDFIGDNGKITPARLTGTKAHYQRQLDTAIKRARFLALMPYTDLHKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="107" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LLZ9</accession>
    <name>Q1LLZ9_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Histidine kinase</fullName>
            <ecNumber evidence="2">2.7.13.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">ompB</name>
        <name evidence="9" type="ordered locus">Rmet_1948</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.</text>
            <dbReference type="EC" id="2.7.13.3"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.7.13.3" evidence="2"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08827.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516675.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1948"/>
    <dbReference type="EnsemblBacteria" id="ABF08827">
        <property type="protein sequence ID" value="ABF08827"/>
        <property type="gene ID" value="Rmet_1948"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1948"/>
    <dbReference type="eggNOG" id="COG0642">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG3850">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_89_27_4"/>
    <dbReference type="OMA" id="SLFWRTF"/>
    <dbReference type="OrthoDB" id="1031685at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00082">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003660">
        <property type="entry name" value="HAMP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003661">
        <property type="entry name" value="HisK_dim/P"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036097">
        <property type="entry name" value="HisK_dim/P_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032408">
        <property type="entry name" value="RisS_PPD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038421">
        <property type="entry name" value="RisS_PPD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004358">
        <property type="entry name" value="Sig_transdc_His_kin-like_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00672">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00512">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16524">
        <property type="entry name" value="RisS_PPD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00344">
        <property type="entry name" value="BCTRLSENSOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00304">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00388">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47384">
        <property type="entry name" value="SSF47384"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="SSF55874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50885">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 9" id="KW-0418">Kinase</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="16"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="163"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="domain" description="HAMP" evidence="8">
        <location>
            <begin position="183"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="7">
        <location>
            <begin position="243"/>
            <end position="449"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ABF08827.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="449" mass="50126" checksum="D892DA2D8066F032" modified="2006-05-30" version="1">MATALGRTATRFFGSLFWRTFMLIALLLAISLGIWFQSYRLFERAPRAQQIAMQVVSVVKLTRAALLYSDPARRRFLLLDLVQNEGIKVYPREKDDDFAAPTANPFLTQLVQQEIRGRLGEDTVIATTVNDIPGVWVSFEIEGDDYWVAISPERFERVPGIQWLWWSIAALMLSIIGAAFITARVNYPLKRLANAARQIGSGGDPPPLREQGASEVAQANRSFNQMVHDLRQLDDDRVVMLAGISHDLRTPLTRLRLETEMSPVDNVTRDAMIADIEQMDAIIGQFLNYARPGQEVVEPVDLSTLVQESVGVYAAHDDVRIHVRSNGPVMAMANRMEIQRILDNLVENARRYAKDEETGMAEVEISTRVEDKDALLVVADNGMGVPDEELSLLTRPFYRLDAARSEAKGAGLGMSIVNRILQRNGGRLVLANRPAPKSGLVVSAIFRRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LM03</accession>
    <name>Q1LM03_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Squalene/phytoene synthase</fullName>
            <ecNumber evidence="1">2.5.1.21</ecNumber>
            <ecNumber evidence="1">2.5.1.32</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_1944</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.5.1.21" evidence="1"/>
    <dbReference type="EC" id="2.5.1.32" evidence="1"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08823.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516672.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1944"/>
    <dbReference type="EnsemblBacteria" id="ABF08823">
        <property type="protein sequence ID" value="ABF08823"/>
        <property type="gene ID" value="Rmet_1944"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1944"/>
    <dbReference type="eggNOG" id="COG1562">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037269_1_1_4"/>
    <dbReference type="OMA" id="RKFWLAW"/>
    <dbReference type="OrthoDB" id="1449625at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004310">
        <property type="term" value="F:farnesyl-diphosphate farnesyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016767">
        <property type="term" value="F:geranylgeranyl-diphosphate geranylgeranyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051996">
        <property type="term" value="F:squalene synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016117">
        <property type="term" value="P:carotenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00683">
        <property type="entry name" value="Trans_IPPS_HH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.600.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008949">
        <property type="entry name" value="Isoprenoid_synthase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017828">
        <property type="entry name" value="SQ_synth_HpnD-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002060">
        <property type="entry name" value="Squ/phyt_synthse"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033904">
        <property type="entry name" value="Trans_IPPS_HH"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00494">
        <property type="entry name" value="SQS_PSY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48576">
        <property type="entry name" value="SSF48576"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03465">
        <property type="entry name" value="HpnD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF08823.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="276" mass="31034" checksum="CD63785B27E9DA1F" modified="2006-05-30" version="1">MTPDQYCQDKAAQSGSSFYYSSLLLPSDRRNALTALQAWRQELDAIVNENHDAGVAHQRLDWWRAELRRLYDGGAAHPVSQALQPHLDALPQAEMAEMLAATEMDLAQSRYLDEIGLLRYCRASGGTFGKLTARVLGHTDPRTLDAAEQLGLSLQRVRIVKDIGGDARHGRIYIPVDTLQRLEVPAADILQSRHSDRFVALMREQATQARALYGEALAALPRADRRSQRAQLAFAAIQHALLDEIEASDFQVLNQRIDLTPMRKLWIAWKTWLKNG</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="64" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LMC1</accession>
    <name>Q1LMC1_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Type IV fimbrial biogenesis protein PilZ</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">pilZ</name>
        <name evidence="2" type="ordered locus">Rmet_1826</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08705.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516555.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1826"/>
    <dbReference type="EnsemblBacteria" id="ABF08705">
        <property type="protein sequence ID" value="ABF08705"/>
        <property type="gene ID" value="Rmet_1826"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1826"/>
    <dbReference type="eggNOG" id="COG3215">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_147899_1_0_4"/>
    <dbReference type="OMA" id="CNKIETY"/>
    <dbReference type="OrthoDB" id="1538398at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035438">
        <property type="term" value="F:cyclic-di-GMP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009875">
        <property type="entry name" value="PilZ_domain"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07238">
        <property type="entry name" value="PilZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PilZ" evidence="1">
        <location>
            <begin position="31"/>
            <end position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF07238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF08705.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="136" mass="14528" checksum="A15313A5D398E449" modified="2006-05-30" version="1">MNTPAIAAPTRPAVGPATIPNPATTGRPNVLSLSIKDQAGLYAAYMPFLARGGIFVPSNRPFRLGEQVFLVLSLLDRPQKYQVQGTVAWITPVGTPMKTPGVGVHLPDDDIGRSLKRAVEEILGKTIESGRPTQTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LMM4</accession>
    <name>Q1LMM4_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Propionyl-CoA carboxylase beta chain</fullName>
            <ecNumber evidence="4">6.4.1.3</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">pccB</name>
        <name evidence="4" type="ordered locus">Rmet_1721</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EC" id="6.4.1.3" evidence="4"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08602.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516455.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1721"/>
    <dbReference type="EnsemblBacteria" id="ABF08602">
        <property type="protein sequence ID" value="ABF08602"/>
        <property type="gene ID" value="Rmet_1721"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1721"/>
    <dbReference type="eggNOG" id="COG4799">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018822_0_1_4"/>
    <dbReference type="OMA" id="YAYAECT"/>
    <dbReference type="OrthoDB" id="123223at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004658">
        <property type="term" value="F:propionyl-CoA carboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034733">
        <property type="entry name" value="AcCoA_carboxyl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029045">
        <property type="entry name" value="ClpP/crotonase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011763">
        <property type="entry name" value="COA_CT_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011762">
        <property type="entry name" value="COA_CT_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045190">
        <property type="entry name" value="MCCB/AccD1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22855">
        <property type="entry name" value="PTHR22855"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01039">
        <property type="entry name" value="Carboxyl_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52096">
        <property type="entry name" value="SSF52096"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50989">
        <property type="entry name" value="COA_CT_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50980">
        <property type="entry name" value="COA_CT_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0436">Ligase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CoA carboxyltransferase N-terminal" evidence="2">
        <location>
            <begin position="22"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="domain" description="CoA carboxyltransferase C-terminal" evidence="3">
        <location>
            <begin position="282"/>
            <end position="534"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF08602.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="539" mass="57007" checksum="20419F7E2C72F1B3" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="105" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LMQ3</accession>
    <name>Q1LMQ3_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">DNA-binding transcriptional dual regulator, global regulator of anaerobic growth</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">fnr</name>
        <name evidence="4" type="ordered locus">Rmet_1692</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08573.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516423.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1692"/>
    <dbReference type="EnsemblBacteria" id="ABF08573">
        <property type="protein sequence ID" value="ABF08573"/>
        <property type="gene ID" value="Rmet_1692"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1692"/>
    <dbReference type="eggNOG" id="COG0664">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_075053_0_2_4"/>
    <dbReference type="OMA" id="FCSTCAF"/>
    <dbReference type="OrthoDB" id="1587662at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00038">
        <property type="entry name" value="CAP_ED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018490">
        <property type="entry name" value="cNMP-bd-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012318">
        <property type="entry name" value="HTH_CRP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00027">
        <property type="entry name" value="cNMP_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13545">
        <property type="entry name" value="HTH_Crp_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00034">
        <property type="entry name" value="HTHCRP"/>
    </dbReference>
    <dbReference type="SMART" id="SM00100">
        <property type="entry name" value="cNMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00419">
        <property type="entry name" value="HTH_CRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51206">
        <property type="entry name" value="SSF51206"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51063">
        <property type="entry name" value="HTH_CRP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="2">
        <location>
            <begin position="14"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="domain" description="HTH crp-type" evidence="3">
        <location>
            <begin position="148"/>
            <end position="221"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF08573.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="244" mass="26901" checksum="62155B0B98D8DCC7" modified="2006-05-30" version="1">MPRCSACTFASFCLPGGLNRRDIDRLDSVVHGRISLHKGESLYRLGDPVTSIYAIRVGTLKSQVSTQDGRVQIVGFHLPGELVGLDSLVSPQYMSHAVALEDARLCRINLTALRDLAVALPGLYNNILRLMANEVRHDHAMLRTLGVLNAEERLIAFLLSLSARLSARGFAASEFQLRMTREEIGSYLGLKLETISRLFSRLSETGLITVRHRSVKLNDLNGLRLIYEGSSGALASFQNHCLAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LMW0</accession>
    <name>Q1LMW0_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Alpha-dehydro-beta-deoxy-D-glucarate aldolase</fullName>
            <ecNumber evidence="2">4.1.2.20</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">garL</name>
        <name evidence="2" type="ordered locus">Rmet_1633</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EC" id="4.1.2.20" evidence="2"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08516.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516361.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1633"/>
    <dbReference type="EnsemblBacteria" id="ABF08516">
        <property type="protein sequence ID" value="ABF08516"/>
        <property type="gene ID" value="Rmet_1633"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1633"/>
    <dbReference type="eggNOG" id="COG3836">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_059964_1_0_4"/>
    <dbReference type="OMA" id="NEGWMVK"/>
    <dbReference type="OrthoDB" id="1797448at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008672">
        <property type="term" value="F:2-dehydro-3-deoxyglucarate aldolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019394">
        <property type="term" value="P:glucarate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005000">
        <property type="entry name" value="Aldolase/citrate-lyase_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017648">
        <property type="entry name" value="GarL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015813">
        <property type="entry name" value="Pyrv/PenolPyrv_Kinase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040442">
        <property type="entry name" value="Pyrv_Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03328">
        <property type="entry name" value="HpcH_HpaI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51621">
        <property type="entry name" value="SSF51621"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03239">
        <property type="entry name" value="GarL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0456">Lyase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HpcH_HpaI" evidence="1">
        <location>
            <begin position="26"/>
            <end position="250"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF08516.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="261" mass="28722" checksum="314EAF454BD10BC8" modified="2006-05-30" version="1">MTQHLPQITPLPNRFRADVLARKRLIGLWSSLGQPVTTEIIGLAGFDWLLLDGEHSPNDVLTFMPQLMALKDSTSAPVVRPPWNDPVMLKRLLDIGFYNFLIPFVESAEEARRAVRATRYPPDGIRGVSGAQRQNRYGTLPDFFKTINDNICVLVQIESRKGVEAADEICAEPGVDGIFIGPNDLAAAYGHLGNPNHPEVQAAVRQIYEAANRAGKAIGTLTPVEADAKRYLDMGAHFVAVGIDNALLRDAARALRERFTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="67" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LMX4</accession>
    <name>Q1LMX4_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_1619</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08502.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516348.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1619"/>
    <dbReference type="EnsemblBacteria" id="ABF08502">
        <property type="protein sequence ID" value="ABF08502"/>
        <property type="gene ID" value="Rmet_1619"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1619"/>
    <dbReference type="eggNOG" id="COG1917">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_130991_3_0_4"/>
    <dbReference type="OMA" id="MDYVIVP"/>
    <dbReference type="OrthoDB" id="1928636at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011051">
        <property type="entry name" value="RmlC_Cupin_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51182">
        <property type="entry name" value="SSF51182"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF08502.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="99" mass="10924" checksum="FA6E41335ED30679" modified="2006-05-30" version="1">MTRPQAVPTVQVDNERVAVTEWRFAKGAETGHHHHGYDYVVVPMTTGPLLLQTPEGEVTSQLVAGRAYYRAAGVEHNVINAHDGECVFVEIEIKRGDQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="63" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LNN4</accession>
    <name>Q1LNN4_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Transcriptional regulator of Polyhydroxyalkanoate synthesis (Repressor)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">phaR</name>
        <name evidence="3" type="ordered locus">Rmet_1359</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08242.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516122.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1359"/>
    <dbReference type="EnsemblBacteria" id="ABF08242">
        <property type="protein sequence ID" value="ABF08242"/>
        <property type="gene ID" value="Rmet_1359"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1359"/>
    <dbReference type="eggNOG" id="COG5394">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_089210_1_0_4"/>
    <dbReference type="OMA" id="MQGMMGS"/>
    <dbReference type="OrthoDB" id="1665699at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012909">
        <property type="entry name" value="PHA_DNA-bd_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010134">
        <property type="entry name" value="PHA_reg_PhaR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007897">
        <property type="entry name" value="PHB_accumulat"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05233">
        <property type="entry name" value="PHB_acc"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07879">
        <property type="entry name" value="PHB_acc_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01848">
        <property type="entry name" value="PHA_reg_PhaR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PHB_acc_N" evidence="2">
        <location>
            <begin position="11"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="domain" description="PHB_acc" evidence="1">
        <location>
            <begin position="75"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="domain" description="PHB_acc" evidence="1">
        <location>
            <begin position="126"/>
            <end position="165"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF05233"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF08242.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="183" mass="20936" checksum="DBE9555F31BD02D0" modified="2006-05-30" version="1">MATSKKGAERLIKKYPNRRLYDTQTSTYITLADVKQLVMDSEEFKVVDAKSGEELTRSILLQIILEEETGGVPMFSSAMLSQIIRFYGHAMQGMMGTYLEKNIQAFIDIQSKLAENSKGLYSGEPFSPDMWSQFMNLQGPMMQGMMSNYIEQSKNLFVQMQEQMQNQAKSMFGTFPFNQPDKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="61" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LNP2</accession>
    <name>Q1LNP2_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_1351</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08234.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516115.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1351"/>
    <dbReference type="EnsemblBacteria" id="ABF08234">
        <property type="protein sequence ID" value="ABF08234"/>
        <property type="gene ID" value="Rmet_1351"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1351"/>
    <dbReference type="eggNOG" id="COG5639">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_179036_1_0_4"/>
    <dbReference type="OMA" id="MTLIPHM"/>
    <dbReference type="OrthoDB" id="1902903at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018733">
        <property type="entry name" value="DUF2274"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10038">
        <property type="entry name" value="DUF2274"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF08234.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="81" mass="8760" checksum="098C3E1F1577D2D3" modified="2006-05-30" version="1">MSATKKLRLGPLPKTENVKLTFACPASLKADLDRYAALHAQAYGEAVDAEKLIPHMLEAFMAGDRGFRKGTTTRSAPSKPT</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LNQ7</accession>
    <name>Q1LNQ7_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Transcriptional regulator, LysR family (CMGI-2)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">traR</name>
        <name evidence="4" type="ordered locus">Rmet_1336</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the LysR transcriptional regulatory family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08219.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1336"/>
    <dbReference type="EnsemblBacteria" id="ABF08219">
        <property type="protein sequence ID" value="ABF08219"/>
        <property type="gene ID" value="Rmet_1336"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1336"/>
    <dbReference type="eggNOG" id="COG0583">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039613_6_4_4"/>
    <dbReference type="OMA" id="EIRFFEV"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005119">
        <property type="entry name" value="LysR_subst-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000847">
        <property type="entry name" value="Tscrpt_reg_HTH_LysR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00126">
        <property type="entry name" value="HTH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03466">
        <property type="entry name" value="LysR_substrate"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00039">
        <property type="entry name" value="HTHLYSR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50931">
        <property type="entry name" value="HTH_LYSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH lysR-type" evidence="3">
        <location>
            <begin position="7"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="301"/>
            <end position="320"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF08219.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="320" mass="35577" checksum="5BC860CA0E8462EB" modified="2006-05-30" version="1">MNRKGTVELRHLRCFIAVAEELHFARAAERLHIEQSPLSRAIKELEEDLGVQLFERTTRSTRLTRAGQLFLEHVPRIFTALQQARDSVKAVANGFHGQLRVALSDGITPSRLPALLALCRQEEPEVEIRFFEVPLSQQIKGLHDDLYDVGFAQSDEVGDGIVALPAWSDALMVAVPARHPLLIHKRIPLDELLRYPLVLCDPQVCEGLAKHVERVLRRVDMEPLVAERVATSDLMMALVSAGFALGLAGAAHIAASRESGVVARPLASRVPPLTTYLLRLDSDPSDELARFIERVKAIDLPETPRPARGRNPDHPEELMP</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-10-07" version="63" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LPE6</accession>
    <name>Q1LPE6_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_1094</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07980.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1094"/>
    <dbReference type="EnsemblBacteria" id="ABF07980">
        <property type="protein sequence ID" value="ABF07980"/>
        <property type="gene ID" value="Rmet_1094"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1094"/>
    <dbReference type="eggNOG" id="ENOG502Z8D0">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_057858_0_0_4"/>
    <dbReference type="OMA" id="PFMNVYG"/>
    <dbReference type="OrthoDB" id="875447at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193726" evidence="1">
        <location>
            <begin position="29"/>
            <end position="349"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF07980.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="349" mass="37810" checksum="9F69F20C931FF927" modified="2006-05-30" version="1">MGRSVLRGVLPRYLVFLAFAASAFAAHAWTAGSDGGAGAATASPDTESGPYRGIVGPVGTAEPAPEAPKEWDRALPFFAQRVIDKGYNLPNPYNIGYSYYNGYQRYQLSDLAVSAGSNPLRAADFVQFQQSKLHNVSNQFQLGAWIFPFLNVYGMIGTIEGNGAIDISFSSKTDLQQFFGINIGCGGSRPRPECSNPIKLPTQFANYSGHTFGGGFTLVGTYKQLFFSLPVTYTVSDITMSETPVQSINIGPRIGWNFRLGPGLGLLTPFVGVTYFRTHAKITGHFDVPIQDAGGQTARLNYEINQSVVGAWSGSAGVSWAVNRSLGFIMEFGYGYNRNNIIATGFFRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="96" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LQ56</accession>
    <name>Q1LQ56_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Glucarate dehydratase</fullName>
            <ecNumber evidence="5">4.2.1.40</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">gudD</name>
        <name evidence="13" type="ordered locus">Rmet_0834</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>D-glucarate = 5-dehydro-4-deoxy-D-glucarate + H2O</text>
            <dbReference type="Rhea" id="RHEA:14573"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30612"/>
            <dbReference type="ChEBI" id="CHEBI:42819"/>
            <dbReference type="EC" id="4.2.1.40"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 11">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Carbohydrate acid metabolism; D-glucarate degradation; 2,5-dioxopentanoate from D-glucarate: step 1/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the mandelate racemase/muconate lactonizing enzyme family. GlucD subfamily.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.40" evidence="5"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07720.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515662.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0834"/>
    <dbReference type="EnsemblBacteria" id="ABF07720">
        <property type="protein sequence ID" value="ABF07720"/>
        <property type="gene ID" value="Rmet_0834"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0834"/>
    <dbReference type="eggNOG" id="COG4948">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030273_9_0_4"/>
    <dbReference type="OMA" id="MQYLIPN"/>
    <dbReference type="OrthoDB" id="951991at2"/>
    <dbReference type="UniPathway" id="UPA00564">
        <property type="reaction ID" value="UER00627"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008872">
        <property type="term" value="F:glucarate dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042838">
        <property type="term" value="P:D-glucarate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd03323">
        <property type="entry name" value="D-glucarate_dehydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.390.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036849">
        <property type="entry name" value="Enolase-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029017">
        <property type="entry name" value="Enolase-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029065">
        <property type="entry name" value="Enolase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017653">
        <property type="entry name" value="Glucarate_dehydratase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034598">
        <property type="entry name" value="GlucD-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013342">
        <property type="entry name" value="Mandelate_racemase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034593">
        <property type="entry name" value="Mandelate_racemase_DgoD-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013341">
        <property type="entry name" value="Mandelate_racemase_N_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48080">
        <property type="entry name" value="PTHR48080"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13378">
        <property type="entry name" value="MR_MLE_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02746">
        <property type="entry name" value="MR_MLE_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00005">
        <property type="entry name" value="glucarate_dehydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00922">
        <property type="entry name" value="MR_MLE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51604">
        <property type="entry name" value="SSF51604"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54826">
        <property type="entry name" value="SSF54826"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03247">
        <property type="entry name" value="glucar-dehydr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 13" id="KW-0456">Lyase</keyword>
    <keyword evidence="7 11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MR_MLE" evidence="12">
        <location>
            <begin position="191"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="10">
        <location>
            <begin position="241"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="10">
        <location>
            <begin position="345"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="9">
        <location>
            <position position="213"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="9">
        <location>
            <position position="345"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="38"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="109"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="156"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="211"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="241"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="272"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10 11">
        <location>
            <position position="295"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="374"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="427"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001426"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005183"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009938"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011973"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR617653-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR617653-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR617653-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM00922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ABF07720.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="451" mass="49306" checksum="75005A353B26159C" modified="2006-05-30" version="1">MNAASLQGAAGATPVVTELTVVPVAGHDSMLMNLSGAHGPYFTRNIVILRDSAGNTGVGEVPGGEGIRKTLEDARPLVVGQPIGQYQAMLNKVRATFASRDAGGRGLQTFDLRITIHAVTALEAALLDLLGKHLQVPVAALLGEGQQRDAVEMLGYLFYVGDRQRTTLDYRTEPDADNEWFRLRNEVAMDAKGVVRLAEAAYERYGFNDFKLKGGVLSGDEEMEAILALHERFPKARVTLDPNGGWLLADAIRLCRDKHGVLAYAEDPCGAEDGYSGREIMAEFRTATGLPTATNMIATDWRQMGHAVRLHSVDIPLADPHFWTMQGSVRVAQMCAEWGLTWGSHSNNHFDISLAMFTHVAAAAPGRVTAIDTHWIWQDGEHLTRNPLRIEGGLVQVPKTPGLGVELDMDALARANRLYQEKGLGARDDAIAMQFLIPGWKFNNKMPCMVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="72" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LQF7</accession>
    <name>Q1LQF7_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Deacetylase, histone deacetylase family</fullName>
            <ecNumber evidence="3">3.1.1.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_0733</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the histone deacetylase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.-" evidence="3"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07619.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008643671.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0733"/>
    <dbReference type="EnsemblBacteria" id="ABF07619">
        <property type="protein sequence ID" value="ABF07619"/>
        <property type="gene ID" value="Rmet_0733"/>
    </dbReference>
    <dbReference type="GeneID" id="60825220"/>
    <dbReference type="KEGG" id="rme:Rmet_0733"/>
    <dbReference type="eggNOG" id="COG0123">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007727_8_1_4"/>
    <dbReference type="OMA" id="GRAMGFC"/>
    <dbReference type="OrthoDB" id="1443295at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.800.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000286">
        <property type="entry name" value="His_deacetylse"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023801">
        <property type="entry name" value="His_deacetylse_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037138">
        <property type="entry name" value="His_deacetylse_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023696">
        <property type="entry name" value="Ureohydrolase_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00850">
        <property type="entry name" value="Hist_deacetyl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01270">
        <property type="entry name" value="HDASUPER"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52768">
        <property type="entry name" value="SSF52768"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Hist_deacetyl" evidence="2">
        <location>
            <begin position="20"/>
            <end position="303"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF07619.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="307" mass="33774" checksum="A8DF1C57637C2C1D" modified="2006-05-30" version="1">MSTGYYTHPSFLMHEMGHFHPECPERLQAIEDHLISHGMDGLLDRREAPAATDAQIERVHRPEYVASLVAMSPATGYHPIDPDTLMNPHTLTAARHAAGAAVAATDAVIAGELENAFCCARPPGHHAEPDRAMGFCFFDNVAIAARHALTAHGLSRVAIVDFDVHHGNGTEAAFRDDPQVLMCSFFQHPFYPYSGTEHFAPNMSNIPLPAYSNGLAVREVVETIWLPRLNEFKPEMLFISAGFDAHREDDLGQMGLVEQDYAWITQQLVDVARAHAKGRIVSCLEGGYNLSALARSVFAHLKVLVET</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LQH2</accession>
    <name>Q1LQH2_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Prephenate dehydrogenase</fullName>
            <ecNumber evidence="2">1.3.1.12</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">tyrA</name>
        <name evidence="2" type="ordered locus">Rmet_0718</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EC" id="1.3.1.12" evidence="2"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07604.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515567.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0718"/>
    <dbReference type="EnsemblBacteria" id="ABF07604">
        <property type="protein sequence ID" value="ABF07604"/>
        <property type="gene ID" value="Rmet_0718"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0718"/>
    <dbReference type="eggNOG" id="COG0287">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_055968_0_1_4"/>
    <dbReference type="OMA" id="MWRDICL"/>
    <dbReference type="OrthoDB" id="533829at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008977">
        <property type="term" value="F:prephenate dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004665">
        <property type="term" value="F:prephenate dehydrogenase (NADP+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006571">
        <property type="term" value="P:tyrosine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008927">
        <property type="entry name" value="6-PGluconate_DH-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003099">
        <property type="entry name" value="Prephen_DH"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02153">
        <property type="entry name" value="PDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48179">
        <property type="entry name" value="SSF48179"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51176">
        <property type="entry name" value="PDH_ADH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Prephenate/arogenate dehydrogenase" evidence="1">
        <location>
            <begin position="11"/>
            <end position="298"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF07604.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="302" mass="31477" checksum="3A7240004DDFA401" modified="2006-05-30" version="1">MSETVSALHFSKVVIVGVGLIGGSLALSLKRAGVVGTVVGVGRSAASLDKAKALGVIDEAASLEVAMQGASLVVLCAPVAQNFALLHALEPHLQPGTIVTDAGSTKSDVIMAAKTALGDKVAQFVPAHPIAGRELNGVEAALDDLYVGKKVVLCPLQENSRADVAAVRAMWEATGADCHVMSAVQHDAVFASVSHLPHLLSYALVAQVANAEDAALKLDFAGGGFRDFTRIAASSPEMWRDICIANKDALLREISIYESVLAHLKTKIKEGDGAALERLFARASDTRLKWGAARTAANNVEP</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LQR3</accession>
    <name>Q1LQR3_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Putative Phospholipase D family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Rmet_0627</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07513.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515484.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0627"/>
    <dbReference type="EnsemblBacteria" id="ABF07513">
        <property type="protein sequence ID" value="ABF07513"/>
        <property type="gene ID" value="Rmet_0627"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0627"/>
    <dbReference type="eggNOG" id="COG1502">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013419_1_0_4"/>
    <dbReference type="OMA" id="LNICHEH"/>
    <dbReference type="OrthoDB" id="1881748at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019637">
        <property type="term" value="P:organophosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025202">
        <property type="entry name" value="PLD-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001736">
        <property type="entry name" value="PLipase_D/transphosphatidylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015679">
        <property type="entry name" value="PLipase_D_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18896">
        <property type="entry name" value="PTHR18896"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13091">
        <property type="entry name" value="PLDc_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00155">
        <property type="entry name" value="PLDc"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50035">
        <property type="entry name" value="PLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PLD phosphodiesterase" evidence="4">
        <location>
            <begin position="548"/>
            <end position="575"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="626"/>
            <end position="655"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="448"/>
            <end position="514"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="626"/>
            <end position="640"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABF07513.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="655" mass="73436" checksum="70630C1D463BDEB2" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LQS3</accession>
    <name>Q1LQS3_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">GPW_gp25 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_0617</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07503.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515474.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0617"/>
    <dbReference type="EnsemblBacteria" id="ABF07503">
        <property type="protein sequence ID" value="ABF07503"/>
        <property type="gene ID" value="Rmet_0617"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0617"/>
    <dbReference type="eggNOG" id="COG3518">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_132637_0_0_4"/>
    <dbReference type="OMA" id="YFEPPGR"/>
    <dbReference type="OrthoDB" id="1918415at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007048">
        <property type="entry name" value="IraD/Gp25-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017737">
        <property type="entry name" value="TssE1-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04965">
        <property type="entry name" value="GPW_gp25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03357">
        <property type="entry name" value="VI_zyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GPW_gp25" evidence="1">
        <location>
            <begin position="34"/>
            <end position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF04965"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF07503.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="144" mass="16095" checksum="204BEE0FE73566E4" modified="2006-05-30" version="1">MTRGGPGLFEAVTGFFANGVAVDELDASTQTFLSVQDNIQRILNSRRGGLAHLPDYGLGDLSRIYRHLPASAHTLKREIETTLLRYEPRLKALDLEIEEPEPGMLLSFTMTCHLHQSGLVQFGTHFMPDGKTRLKLLRSAHDHD</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LQU0</accession>
    <name>Q1LQU0_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Multidrug efflux system, subunit B</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">mdtB</name>
        <name evidence="4" type="ordered locus">Rmet_0600</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07486.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515459.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0600"/>
    <dbReference type="EnsemblBacteria" id="ABF07486">
        <property type="protein sequence ID" value="ABF07486"/>
        <property type="gene ID" value="Rmet_0600"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0600"/>
    <dbReference type="eggNOG" id="COG0841">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002755_1_2_4"/>
    <dbReference type="OMA" id="GQDFSIM"/>
    <dbReference type="OrthoDB" id="145346at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2090.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027463">
        <property type="entry name" value="AcrB_DN_DC_subdom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001036">
        <property type="entry name" value="Acrflvin-R"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000424">
        <property type="entry name" value="Primosome_PriB/ssb"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32063">
        <property type="entry name" value="PTHR32063"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00873">
        <property type="entry name" value="ACR_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00702">
        <property type="entry name" value="ACRIFLAVINRP"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82714">
        <property type="entry name" value="SSF82714"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50935">
        <property type="entry name" value="SSB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="348"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="372"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="443"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="475"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="543"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="873"/>
            <end position="890"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="923"/>
            <end position="949"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="970"/>
            <end position="989"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="1001"/>
            <end position="1027"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1040"/>
            <end position="1066"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00252"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF07486.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="1066" mass="113935" checksum="E199E2FA7A393EBA" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LQV4</accession>
    <name>Q1LQV4_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Predicted ornithine cyclodeaminase</fullName>
            <ecNumber evidence="1">4.3.1.12</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">arcB</name>
        <name evidence="1" type="ordered locus">Rmet_0586</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EC" id="4.3.1.12" evidence="1"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07472.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515446.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0586"/>
    <dbReference type="EnsemblBacteria" id="ABF07472">
        <property type="protein sequence ID" value="ABF07472"/>
        <property type="gene ID" value="Rmet_0586"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0586"/>
    <dbReference type="eggNOG" id="COG2423">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_042088_3_2_4"/>
    <dbReference type="OMA" id="DMIEPGM"/>
    <dbReference type="OrthoDB" id="1193876at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008473">
        <property type="term" value="F:ornithine cyclodeaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1780.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003462">
        <property type="entry name" value="ODC_Mu_crystall"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023401">
        <property type="entry name" value="ODC_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13812">
        <property type="entry name" value="PTHR13812"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02423">
        <property type="entry name" value="OCD_Mu_crystall"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0456">Lyase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF07472.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="366" mass="39368" checksum="1236848B2BED19A7" modified="2006-05-30" version="1">MKHALKETGMTRFLDAGDVATLVRRIGVPVAIMQMADCVRQDFLRWDAFEKSARLASHSPVGVIELMPVSDGVQFAFKYVNGHPRNARHALPTVMACGMLAEVETGFPLLLADLTLATALRTAATSVLAARAMARPGARTMALIGNGAQAEFQSLAFHAGLGIETVRAFDIDAHATARLMRNFAAVPGLNIVPTRSVREALDSADIVTTVTADKTRATILTPDMIRPGMHLNAVGGDCPGKTELHVDILHRARIMVEYAPQSRIEGEIQQWPEAPVSELWQVLSGAVPGRVSADDVTIFDSVGFALEDYSALRWLHAAAIAHHAGQFIELVALPPDPRDLYGWMMKPDIAMPGAMSDVPGKAVALA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LQW3</accession>
    <name>Q1LQW3_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Putative nucleoside 2-deoxyribosyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_0577</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07463.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515438.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0577"/>
    <dbReference type="EnsemblBacteria" id="ABF07463">
        <property type="protein sequence ID" value="ABF07463"/>
        <property type="gene ID" value="Rmet_0577"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0577"/>
    <dbReference type="eggNOG" id="COG3613">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_104078_0_0_4"/>
    <dbReference type="OMA" id="YELGYMA"/>
    <dbReference type="OrthoDB" id="1773352at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007710">
        <property type="entry name" value="Nucleoside_deoxyribTrfase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05014">
        <property type="entry name" value="Nuc_deoxyrib_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF07463.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="173" mass="18874" checksum="601C954E02287664" modified="2006-05-30" version="1">MKMVYLAGFDVFREDARDWGEQLKALCMRYGYEGLYPLDKAAPSGLSGSATAQWIYEANIALIRRADVVMANLDDFRGSGEPDSGTAFEVGFAVALEKPVWGYAADAGTLLDRVRVRTDADGNARDARGYVVEDFGLSMNLMLACSVRRVTGDAEACLAAMAEADRQLAVRRD</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-01-15" modified="2022-08-03" version="96" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LR08</accession>
    <name>KAD_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Adenylate kinase</fullName>
            <shortName evidence="1">AK</shortName>
            <ecNumber evidence="1">2.7.4.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">ATP-AMP transphosphorylase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">ATP:AMP phosphotransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Adenylate monophosphate kinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">adk</name>
        <name type="ordered locus">Rmet_0532</name>
    </gene>
    <organism>
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the reversible transfer of the terminal phosphate group between ATP and AMP. Plays an important role in cellular energy homeostasis and in adenine nucleotide metabolism.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>AMP + ATP = 2 ADP</text>
            <dbReference type="Rhea" id="RHEA:12973"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.3"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Purine metabolism; AMP biosynthesis via salvage pathway; AMP from ADP: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="1">Consists of three domains, a large central CORE domain and two small peripheral domains, NMPbind and LID, which undergo movements during catalysis. The LID domain closes over the site of phosphoryl transfer upon ATP binding. Assembling and dissambling the active center during each catalytic cycle provides an effective means to prevent ATP hydrolysis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the adenylate kinase family.</text>
    </comment>
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        <property type="protein sequence ID" value="ABF07418.1"/>
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        <property type="gene ID" value="Rmet_0532"/>
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        <property type="taxonomic scope" value="Bacteria"/>
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    <dbReference type="HOGENOM" id="CLU_032354_1_2_4"/>
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        <property type="reaction ID" value="UER00649"/>
    </dbReference>
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        <property type="component" value="Chromosome"/>
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        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="term" value="F:adenylate kinase activity"/>
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        <property type="project" value="UniProtKB-UniRule"/>
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        <property type="project" value="UniProtKB-UniPathway"/>
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        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <property type="entry name" value="ADK"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Adenylate_kinase_Adk"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Adenyl_kin_sub"/>
    </dbReference>
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        <property type="entry name" value="Adenylat/UMP-CMP_kin"/>
    </dbReference>
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        <property type="entry name" value="Adenylat_kinase_CS"/>
    </dbReference>
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        <property type="entry name" value="Adenylate_kinase_lid-dom"/>
    </dbReference>
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        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23359">
        <property type="entry name" value="PTHR23359"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ADK_lid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00094">
        <property type="entry name" value="ADENYLTKNASE"/>
    </dbReference>
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        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="adk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00113">
        <property type="entry name" value="ADENYLATE_KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0418">Kinase</keyword>
    <keyword id="KW-0545">Nucleotide biosynthesis</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_1000021763" description="Adenylate kinase">
        <location>
            <begin position="1"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="region of interest" description="NMP" evidence="1">
        <location>
            <begin position="30"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="region of interest" description="LID" evidence="1">
        <location>
            <begin position="122"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="10"/>
            <end position="15"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="31"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="36"/>
        </location>
        <ligand>
            <name>AMP</name>
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        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="57"/>
            <end position="59"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="85"/>
            <end position="88"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="92"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="123"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="132"/>
            <end position="133"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="156"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="167"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="207"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00235"/>
        </source>
    </evidence>
    <sequence length="221" mass="24144" checksum="014681ACAF3F969C" modified="2006-05-30" version="1">MRLILLGAPGAGKGTQAKFICEKFGIPQISTGDMLRAAVKAGTPLGIEAKKVMDAGGLVSDDIIIGLVKDRLKQPDCEKGYLFDGFPRTIPQAEAMKEAGVAIDYVLEIDVPFDAIIERMSGRRVHVASGRTYHVKFNPPKADMVDDETGEALIQRDDDKEETVRKRLDVYSQQTRPLVDYYSNWAANGDASAKVSPPKYRKIAGLGEVDKITASVFDALK</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LR18</accession>
    <name>Q1LR18_CUPMC</name>
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        <recommendedName>
            <fullName evidence="2">Chalcone_isomerase domain-containing protein</fullName>
        </recommendedName>
    </protein>
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        <name evidence="3" type="ordered locus">Rmet_0522</name>
    </gene>
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        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
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            <taxon>Betaproteobacteria</taxon>
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        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
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                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07408.1"/>
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    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0522"/>
    <dbReference type="EnsemblBacteria" id="ABF07408">
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        <property type="gene ID" value="Rmet_0522"/>
    </dbReference>
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    <dbReference type="eggNOG" id="ENOG502ZD7C">
        <property type="taxonomic scope" value="Bacteria"/>
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    <dbReference type="OMA" id="IWLGEKP"/>
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        <property type="component" value="Chromosome"/>
    </dbReference>
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        <property type="project" value="InterPro"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Chalcone_isomerase_sf"/>
    </dbReference>
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        <property type="entry name" value="Chalcone_3"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SSF54626"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
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        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193648" description="Chalcone_isomerase domain-containing protein" evidence="1">
        <location>
            <begin position="37"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="domain" description="Chalcone_isomerase" evidence="2">
        <location>
            <begin position="36"/>
            <end position="204"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF16036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF07408.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="205" mass="22359" checksum="014F0B1878C75D3E" modified="2006-05-30" version="1">MKSLKSPSRTTALSRRFAATLCATLALWTLMPPASAMVIEGVRFDDATRLGGKELALNGAGLRSVFVIKGYVAGLYLPERARNATVALATRGPKRLQLRVLREVEAESFIKALNEGLRENHSELQMRMLADRLAQLQQTMTEMGAAHRGDIINFDFTPEGGTVVALNGIPRGQAIAGEDFYQAVLRIFLGDHPVDRNLKRGLLGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="99" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LR81</accession>
    <name>Q1LR81_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Putative two component transcriptional regulator, LuxR family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Rmet_0459</name>
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    <organism evidence="5 6">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
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        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07345.1"/>
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        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0459"/>
    <dbReference type="EnsemblBacteria" id="ABF07345">
        <property type="protein sequence ID" value="ABF07345"/>
        <property type="gene ID" value="Rmet_0459"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0459"/>
    <dbReference type="eggNOG" id="COG2197">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_90_1_4"/>
    <dbReference type="OMA" id="DDHPLVC"/>
    <dbReference type="OrthoDB" id="1687028at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
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        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06170">
        <property type="entry name" value="LuxR_C_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016032">
        <property type="entry name" value="Sig_transdc_resp-reg_C-effctor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000792">
        <property type="entry name" value="Tscrpt_reg_LuxR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00196">
        <property type="entry name" value="GerE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00038">
        <property type="entry name" value="HTHLUXR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00421">
        <property type="entry name" value="HTH_LUXR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46894">
        <property type="entry name" value="SSF46894"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00622">
        <property type="entry name" value="HTH_LUXR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50043">
        <property type="entry name" value="HTH_LUXR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Response regulatory" evidence="4">
        <location>
            <begin position="3"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="domain" description="HTH luxR-type" evidence="3">
        <location>
            <begin position="139"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="2">
        <location>
            <position position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABF07345.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="207" mass="21890" checksum="E92990AB49EDB23F" modified="2006-05-30" version="1">MSNALVIDNHPGVRAAASAALMESMGFEEVRLATTATEALEELRTRQRPDLVMVDLDLGDLPGDAVVEEVVSHHPATRVLVLSAASGAAERALLSGAHGYVDKGNGIGGIEHAVRAVMSGYAAFPLALLTTIRRAANAPADPILVLSRRELTVMRFLAAGHSNKAISVVLGISNKTVSSHKASIMTKLGFRSLIDLAEFARRHHLAC</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="72" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LRE1</accession>
    <name>Q1LRE1_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Glutamate and aspartate transporter subunit periplasmic-binding component of ABC superfamily</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">gltI</name>
        <name evidence="4" type="ordered locus">Rmet_0399</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07285.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515277.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0399"/>
    <dbReference type="EnsemblBacteria" id="ABF07285">
        <property type="protein sequence ID" value="ABF07285"/>
        <property type="gene ID" value="Rmet_0399"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0399"/>
    <dbReference type="eggNOG" id="COG0834">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019602_0_0_4"/>
    <dbReference type="OMA" id="PNQLDCL"/>
    <dbReference type="OrthoDB" id="685252at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001638">
        <property type="entry name" value="Solute-binding_3/MltF_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00497">
        <property type="entry name" value="SBP_bac_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00062">
        <property type="entry name" value="PBPb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193661" evidence="2">
        <location>
            <begin position="23"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="domain" description="PBPb" evidence="3">
        <location>
            <begin position="38"/>
            <end position="270"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00062"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF07285.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="299" mass="32940" checksum="6A8B7950B15F554E" modified="2006-05-30" version="1">MNVAKLAGLMIAAGVLCGNAQAAEQLTGTLQKIKDTGVITLGVRDSSIPFNYNLGGVRQVGYSYDINMKIVEAIKDQLKLPNLQVKEIPITSQNRITLLQNGTIDIECGSTTNNLERQKQVSFTDTIFIIGTRIMVKKDGGIKDWADLKGKNVVTTAGTTSERLLRKMNDDQKLGMNIISTKDHGQSFLTLESGRAVAFMMDDALLYGERAKAKNPNDWIVVGKPQSREAYGCMIRKDDAPFKKVSDQVIAGLMKDGSINTMYTKWFMQPVPPKGLNLDFPLSDDMKALFKTPNDKALD</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="97" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LRE5</accession>
    <name>Q1LRE5_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Transcriptional regulator, AsnC-family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">ybaO</name>
        <name evidence="5" type="ordered locus">Rmet_0395</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07281.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515272.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0395"/>
    <dbReference type="EnsemblBacteria" id="ABF07281">
        <property type="protein sequence ID" value="ABF07281"/>
        <property type="gene ID" value="Rmet_0395"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0395"/>
    <dbReference type="eggNOG" id="COG1522">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_091233_0_2_4"/>
    <dbReference type="OMA" id="HVSGDYD"/>
    <dbReference type="OrthoDB" id="1844486at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="CDD" id="cd00090">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011991">
        <property type="entry name" value="ArsR-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000485">
        <property type="entry name" value="AsnC-type_HTH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011008">
        <property type="entry name" value="Dimeric_a/b-barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019888">
        <property type="entry name" value="Tscrpt_reg_AsnC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019887">
        <property type="entry name" value="Tscrpt_reg_AsnC/Lrp_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019885">
        <property type="entry name" value="Tscrpt_reg_HTH_AsnC-type_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01037">
        <property type="entry name" value="AsnC_trans_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13404">
        <property type="entry name" value="HTH_AsnC-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00033">
        <property type="entry name" value="HTHASNC"/>
    </dbReference>
    <dbReference type="SMART" id="SM00344">
        <property type="entry name" value="HTH_ASNC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54909">
        <property type="entry name" value="SSF54909"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00519">
        <property type="entry name" value="HTH_ASNC_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50956">
        <property type="entry name" value="HTH_ASNC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH asnC-type" evidence="4">
        <location>
            <begin position="1"/>
            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50956"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABF07281.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="152" mass="17094" checksum="C774897895499B2C" modified="2006-05-30" version="1">MDRYDRQILSILQEDATVPVAEIGEQVGLTSTPCWRRVQKLEESGIIRKRVALLDPAKLNVGVTVFVSVRTNQHSEKWLKTFHGLVASIPEVTEFYRMAGDTDYLLRVVVPDIAAYDAVYKKLIQGTELADVSSSFAMEQIKYTTALPLDYA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-10-07" version="50" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LRL5</accession>
    <name>Q1LRL5_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_0325</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07211.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0325"/>
    <dbReference type="EnsemblBacteria" id="ABF07211">
        <property type="protein sequence ID" value="ABF07211"/>
        <property type="gene ID" value="Rmet_0325"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0325"/>
    <dbReference type="eggNOG" id="ENOG5030P8X">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1936340_0_0_4"/>
    <dbReference type="OMA" id="LERWIRI"/>
    <dbReference type="OrthoDB" id="1535666at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF07211.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="130" mass="15633" checksum="E7BF10C0614A42E9" modified="2006-05-30" version="1">MSERKKACIIAELERWQDRELGTRLTWERVEAFSGYTRQALSRHPDIVYAYQEAKRALADSDRKSRSRLRRDELAYLDQTVVSLRAKIRWYEQLEAQWLQRWQRIANQCLQRGLSIEDLDRPLDPLNRRG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-01-15" modified="2022-05-25" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LRP9</accession>
    <name>LOLB_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Outer-membrane lipoprotein LolB</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">lolB</name>
        <name type="ordered locus">Rmet_0291</name>
    </gene>
    <organism>
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Plays a critical role in the incorporation of lipoproteins in the outer membrane after they are released by the LolA protein.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell outer membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the LolB family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07177.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515179.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q1LRP9"/>
    <dbReference type="SMR" id="Q1LRP9"/>
    <dbReference type="STRING" id="266264.Rmet_0291"/>
    <dbReference type="EnsemblBacteria" id="ABF07177">
        <property type="protein sequence ID" value="ABF07177"/>
        <property type="gene ID" value="Rmet_0291"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0291"/>
    <dbReference type="eggNOG" id="COG3017">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_092816_3_0_4"/>
    <dbReference type="OMA" id="QIRQDGW"/>
    <dbReference type="OrthoDB" id="1797257at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009279">
        <property type="term" value="C:cell outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044874">
        <property type="term" value="P:lipoprotein localization to outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd16326">
        <property type="entry name" value="LolB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00233">
        <property type="entry name" value="LolB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029046">
        <property type="entry name" value="LolA/LolB/LppX"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004565">
        <property type="entry name" value="OM_lipoprot_LolB"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03550">
        <property type="entry name" value="LolB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89392">
        <property type="entry name" value="SSF89392"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00548">
        <property type="entry name" value="lolB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0998">Cell outer membrane</keyword>
    <keyword id="KW-0143">Chaperone</keyword>
    <keyword id="KW-0449">Lipoprotein</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-0564">Palmitate</keyword>
    <keyword id="KW-0653">Protein transport</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0732">Signal</keyword>
    <keyword id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_1000021675" description="Outer-membrane lipoprotein LolB">
        <location>
            <begin position="21"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="131"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="lipid moiety-binding region" description="N-palmitoyl cysteine" evidence="1">
        <location>
            <position position="21"/>
        </location>
    </feature>
    <feature type="lipid moiety-binding region" description="S-diacylglycerol cysteine" evidence="1">
        <location>
            <position position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00233"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="204" mass="22350" checksum="2E92BC027D2948F3" modified="2006-05-30" version="1" precursor="true">MLRSRRLALLCLATPLWLAACASVTPTRGFDASETAASQLYTGRFSANYVRYGRNEGVQGSFRWQEQGRNVRLDLVSPLGQTLAIVTATPSGATLDLPNEAPRNAPEVDSLMEQALGFSLPVSGMRDWLHGRAAPGTPSNVTRDANGRPDTLRQSGWTVRYLAWQDTPENATPTTTLPRRIDMARDGNESPLSVRLVIDPENKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LRT7</accession>
    <name>Q1LRT7_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">RNA-directed DNA polymerase (Reverse transcriptase)</fullName>
            <ecNumber evidence="2">2.7.7.49</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_0253</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.7.7.49" evidence="2"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07139.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008642654.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0253"/>
    <dbReference type="EnsemblBacteria" id="ABF07139">
        <property type="protein sequence ID" value="ABF07139"/>
        <property type="gene ID" value="Rmet_0253"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0253"/>
    <dbReference type="eggNOG" id="COG1403">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG3344">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013584_15_4_4"/>
    <dbReference type="OMA" id="WVFGDRD"/>
    <dbReference type="OrthoDB" id="1581397at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003964">
        <property type="term" value="F:RNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00085">
        <property type="entry name" value="HNHc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030931">
        <property type="entry name" value="Group_II_RT_mat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002711">
        <property type="entry name" value="HNH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003615">
        <property type="entry name" value="HNH_nuc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013597">
        <property type="entry name" value="Mat_intron_G2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025960">
        <property type="entry name" value="RVT_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08388">
        <property type="entry name" value="GIIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01844">
        <property type="entry name" value="HNH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13655">
        <property type="entry name" value="RVT_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00507">
        <property type="entry name" value="HNHc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="SSF56672"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="group_II_RT_mat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0695">RNA-directed DNA polymerase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="1">
        <location>
            <begin position="96"/>
            <end position="332"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF07139.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="571" mass="66038" checksum="73E6882BDE9FC00F" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="58" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LS95</accession>
    <name>Q1LS95_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_0095</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF06981.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515016.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0095"/>
    <dbReference type="EnsemblBacteria" id="ABF06981">
        <property type="protein sequence ID" value="ABF06981"/>
        <property type="gene ID" value="Rmet_0095"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0095"/>
    <dbReference type="eggNOG" id="ENOG5032ZX9">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_146690_0_0_4"/>
    <dbReference type="OMA" id="AQRAYPF"/>
    <dbReference type="OrthoDB" id="2068333at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014991">
        <property type="entry name" value="DUF1840"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08895">
        <property type="entry name" value="DUF1840"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="57"/>
            <end position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF06981.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="106" mass="11712" checksum="D0339FD1384395D1" modified="2006-05-30" version="1">MLITFKSHAAQDLIMMKDLAITLLGIIGKHLGERGVIKADEMPAAIRKLEAAVQDAKKVHPAEATVHPGKDDEREEEPLHLGQRAYPFLDMLRESAKEGADVMWGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LSA3</accession>
    <name>Q1LSA3_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Homocysteine S-methyltransferase</fullName>
            <ecNumber evidence="5">2.1.1.5</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">bhmT</name>
        <name evidence="5" type="ordered locus">Rmet_0087</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <dbReference type="EC" id="2.1.1.5" evidence="5"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF06973.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515008.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0087"/>
    <dbReference type="EnsemblBacteria" id="ABF06973">
        <property type="protein sequence ID" value="ABF06973"/>
        <property type="gene ID" value="Rmet_0087"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0087"/>
    <dbReference type="eggNOG" id="COG0646">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004914_3_0_4"/>
    <dbReference type="OMA" id="AQEDYKM"/>
    <dbReference type="OrthoDB" id="1149455at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047150">
        <property type="term" value="F:betaine-homocysteine S-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003726">
        <property type="entry name" value="HCY_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036589">
        <property type="entry name" value="HCY_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02574">
        <property type="entry name" value="S-methyl_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82282">
        <property type="entry name" value="SSF82282"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50970">
        <property type="entry name" value="HCY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1 3 5" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3 5" id="KW-0808">Transferase</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Hcy-binding" evidence="4">
        <location>
            <begin position="14"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="254"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="319"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="320"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00333"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABF06973.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="355" mass="38356" checksum="B7846CADDC2924ED" modified="2006-05-30" version="1">MSAAQSQPRPYTRAASLPKLLRDRILILDGAMGTMIQRYKLTEADYRGTRFADHRIDVKGNNELLLLTRPQVITEIHEQYLAAGADLIETNTFGATRVAQEDYKMADLAYEMNVEAARLARAACDKYSTPDKPRFVAGAFGPTPKTASISPDVNDPGARNVTFEELRDSYYEQGKALLEGGADVFLVETIFDTLNAKAALFAIDQLFEDTGERVPVMISGTVTDASGRILSGQTVEAFWNSLRHAKPVTFGLNCALGATLMRPYIAELAKVCDAAVSCYPNAGLPNPMSDTGFDETPEVTSALVEEFAASGLVNLVGGCCGTTPDHIAAIAKSVANKTPRTWPGQYREDTAQSAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="117" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LSC3</accession>
    <name>Q1LSC3_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Pyrrolidone-carboxylate peptidase</fullName>
            <ecNumber evidence="9">3.4.19.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">5-oxoprolyl-peptidase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">Pyroglutamyl-peptidase I</fullName>
            <shortName evidence="9">PGP-I</shortName>
            <shortName evidence="9">Pyrase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="primary">pcp1</name>
        <name evidence="9" type="synonym">pcp</name>
        <name evidence="12" type="ordered locus">Rmet_0067</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2 9">Removes 5-oxoproline from various penultimate amino acid residues except L-proline.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 9 10">
            <text>Release of an N-terminal pyroglutamyl group from a polypeptide, the second amino acid generally not being Pro.</text>
            <dbReference type="EC" id="3.4.19.3"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="9">Homotetramer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3 9">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 9">Belongs to the peptidase C15 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.19.3" evidence="9"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF06953.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011514992.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0067"/>
    <dbReference type="MEROPS" id="C15.001"/>
    <dbReference type="EnsemblBacteria" id="ABF06953">
        <property type="protein sequence ID" value="ABF06953"/>
        <property type="gene ID" value="Rmet_0067"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0067"/>
    <dbReference type="eggNOG" id="COG2039">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_043960_4_0_4"/>
    <dbReference type="OMA" id="KLAYNHK"/>
    <dbReference type="OrthoDB" id="1927224at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016920">
        <property type="term" value="F:pyroglutamyl-peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00501">
        <property type="entry name" value="Peptidase_C15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00417">
        <property type="entry name" value="Pyrrolid_peptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000816">
        <property type="entry name" value="Peptidase_C15"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016125">
        <property type="entry name" value="Peptidase_C15-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036440">
        <property type="entry name" value="Peptidase_C15-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029762">
        <property type="entry name" value="PGP-I_bact-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033694">
        <property type="entry name" value="PGPEP1_Cys_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033693">
        <property type="entry name" value="PGPEP1_Glu_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23402">
        <property type="entry name" value="PTHR23402"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01470">
        <property type="entry name" value="Peptidase_C15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015592">
        <property type="entry name" value="Prld-crbxl_pptds"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00706">
        <property type="entry name" value="PYROGLUPTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53182">
        <property type="entry name" value="SSF53182"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00504">
        <property type="entry name" value="pyro_pdase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01334">
        <property type="entry name" value="PYRASE_CYS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01333">
        <property type="entry name" value="PYRASE_GLU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 9 12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6 9" id="KW-0645">Protease</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 9" id="KW-0788">Thiol protease</keyword>
    <feature type="active site" evidence="9 10">
        <location>
            <position position="82"/>
        </location>
    </feature>
    <feature type="active site" evidence="9 11">
        <location>
            <position position="145"/>
        </location>
    </feature>
    <feature type="active site" evidence="9">
        <location>
            <position position="174"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ABF06953.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="222" mass="23306" checksum="7DC9726EC06E15F7" modified="2006-05-30" version="1">MTTRTVLLTGFEPFENEPVNPSWEAVRALDGERVGDALIVARQLPCVFGKAIEAMDALVDTLRPDVVIAVGQAGGRTELSIERVAINVDDARIADNAGAQPIDAPIAKDGPVAYFSTLPIKAIVRDMRAAGVPAMVSQTAGTFVCNHVFYGLMHVLAHRKAGSAAAPTRGGFIHIPYLPEQAARHPGAPSLALDTLIAGLRVAVATSLSTDADIREGGGQLH</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="118" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LSF1</accession>
    <name>Q1LSF1_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Potassium-transporting ATPase ATP-binding subunit</fullName>
            <ecNumber evidence="12">7.2.2.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">ATP phosphohydrolase [potassium-transporting] B chain</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">Potassium-binding and translocating subunit B</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">Potassium-translocating ATPase B chain</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12 14" type="primary">kdpB</name>
        <name evidence="14" type="ordered locus">Rmet_0039</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Part of the high-affinity ATP-driven potassium transport (or Kdp) system, which catalyzes the hydrolysis of ATP coupled with the electrogenic transport of potassium into the cytoplasm. This subunit is responsible for energy coupling to the transport system and for the release of the potassium ions to the cytoplasm.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>ATP + H2O + K(+)(out) = ADP + H(+) + K(+)(in) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:16777"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="7.2.2.6"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="12">The system is composed of three essential subunits: KdpA, KdpB and KdpC.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="12">Cell inner membrane</location>
            <topology evidence="12">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IA subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="7.2.2.6" evidence="12"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF06925.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011514966.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0039"/>
    <dbReference type="EnsemblBacteria" id="ABF06925">
        <property type="protein sequence ID" value="ABF06925"/>
        <property type="gene ID" value="Rmet_0039"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0039"/>
    <dbReference type="eggNOG" id="COG2216">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025728_2_0_4"/>
    <dbReference type="OMA" id="ILWLWFT"/>
    <dbReference type="OrthoDB" id="237367at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005887">
        <property type="term" value="C:integral component of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008556">
        <property type="term" value="F:P-type potassium transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02078">
        <property type="entry name" value="P-type_ATPase_K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1110.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00285">
        <property type="entry name" value="KdpB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023299">
        <property type="entry name" value="ATPase_P-typ_cyto_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018303">
        <property type="entry name" value="ATPase_P-typ_P_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023298">
        <property type="entry name" value="ATPase_P-typ_TM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008250">
        <property type="entry name" value="ATPase_P-typ_transduc_dom_A_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006391">
        <property type="entry name" value="P-type_ATPase_bsu_IA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001757">
        <property type="entry name" value="P_typ_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044492">
        <property type="entry name" value="P_typ_ATPase_HD_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43743">
        <property type="entry name" value="PTHR43743"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00027">
        <property type="entry name" value="p-type_atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="SSF56784"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81653">
        <property type="entry name" value="SSF81653"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81665">
        <property type="entry name" value="SSF81665"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01494">
        <property type="entry name" value="ATPase_P-type"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01497">
        <property type="entry name" value="kdpB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00154">
        <property type="entry name" value="ATPASE_E1_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="12" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="2 12" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="14" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10 12" id="KW-0406">Ion transport</keyword>
    <keyword evidence="6 12" id="KW-0460">Magnesium</keyword>
    <keyword evidence="11 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4 12" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="7 12" id="KW-0630">Potassium</keyword>
    <keyword evidence="3 12" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 12" id="KW-1278">Translocase</keyword>
    <keyword evidence="5 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="1 12" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="68"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="100"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="259"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="299"/>
            <end position="322"/>
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        <location>
            <begin position="677"/>
            <end position="697"/>
        </location>
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    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="717"/>
            <end position="742"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="active site" description="4-aspartylphosphate intermediate" evidence="12">
        <location>
            <position position="353"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="390"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="394"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="426"/>
            <end position="433"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
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    <feature type="binding site" evidence="12">
        <location>
            <position position="448"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
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        <location>
            <position position="579"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
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        <location>
            <position position="583"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
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        </ligand>
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        <source>
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        </source>
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        <source>
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        </source>
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        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
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        <source>
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        </source>
    </evidence>
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        <source>
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        <source>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
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    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ABF06925.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="743" mass="78275" checksum="3DBCC2A50DD5C95B" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="59" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LSI6</accession>
    <name>Q1LSI6_CUPMC</name>
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        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_0004</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
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        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
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    </dbReference>
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    </dbReference>
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        <property type="term" value="F:DNA binding"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Restrct_endonuc_IV_Mrr"/>
    </dbReference>
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    </dbReference>
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        <location>
            <begin position="1"/>
            <end position="172"/>
        </location>
    </feature>
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        <location>
            <begin position="212"/>
            <end position="334"/>
        </location>
    </feature>
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        <source>
            <dbReference type="Pfam" id="PF04471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF18062"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF06890.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="369" mass="40964" checksum="15AC9CDA968A7752" modified="2006-05-30" version="1">MGGFRVTGRGEQKSWVVLFTTGEDKDWPDTLDLSTGKFVYFGDNKTPGHELHETRGGNKVLRYSFERLHAAVNPRADVAPFLVFKKYPLAHGARSVQFKGLAVPGFPSLSSTEDLVAVWKSSEGQRFQNYRAVFTILNAPVLSRAWINDLKAGDLNSSNAPRAWRQWRESGKYSPLAAAPTTNIRSANAQSPDTALKRELLECIWQHYKGAPIAFEAFAARVFQMTDERVVIDEITRGVVDGGRDAIGRYRLGSMADPVYAEFSLEAKCYRPPLNGDTPITVSVSDVARLISRIRHRQFGVLVTTSVIASQAYKEVREDRHPIVFISGGDMVNILIDKGYNTRGRVQELLSSDFALVAAASSEPVDKPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-04-26" modified="2021-06-02" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>Q58AH0</accession>
    <name>Q58AH0_CUPMC</name>
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        <submittedName>
            <fullName evidence="1">Antitoxin of a toxin/antitoxin system</fullName>
        </submittedName>
    </protein>
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        <name evidence="1" type="ordered locus">Rmet_6159</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
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            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="1 2">
        <name>pMOL30</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid pMOL30</plasmid>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_007971.2"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_006466.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF13018">
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        <property type="gene ID" value="Rmet_6159"/>
    </dbReference>
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    <dbReference type="HOGENOM" id="CLU_179376_1_1_4"/>
    <dbReference type="OMA" id="RTFIRVQ"/>
    <dbReference type="OrthoDB" id="2064132at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid pMOL30"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019239">
        <property type="entry name" value="VapB_antitoxin"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09957">
        <property type="entry name" value="VapB_antitoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF13018.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="65" mass="7048" checksum="BCCE6D8AD996A640" modified="2005-04-26" version="1">MRTTIALDDELLAKAQAYTGLSEKTAIVREALKALIQREAAKRLANLGGSQPGIQGAARRRQDIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-04-26" modified="2022-05-25" version="96" xmlns="http://uniprot.org/uniprot">
    <accession>Q58AL4</accession>
    <name>Q58AL4_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Glycosyltransferase (Bactoprenol) involved in cell wall biogenesis family 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">gtrB1</name>
        <name evidence="4" type="ordered locus">Rmet_5968</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4 5">
        <name>pMOL30</name>
    </geneLocation>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="5">Plasmid pMOL30</plasmid>
        </source>
    </reference>
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        <property type="protein sequence ID" value="ABF12827.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_007971.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_145604.1">
        <property type="nucleotide sequence ID" value="NC_006466.1"/>
    </dbReference>
    <dbReference type="CAZy" id="GT2">
        <property type="family name" value="Glycosyltransferase Family 2"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF12827">
        <property type="protein sequence ID" value="ABF12827"/>
        <property type="gene ID" value="Rmet_5968"/>
    </dbReference>
    <dbReference type="GeneID" id="60820434"/>
    <dbReference type="KEGG" id="rme:Rmet_5968"/>
    <dbReference type="HOGENOM" id="CLU_033536_0_1_4"/>
    <dbReference type="OMA" id="NKNYGQT"/>
    <dbReference type="OrthoDB" id="1064289at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid pMOL30"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001173">
        <property type="entry name" value="Glyco_trans_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00535">
        <property type="entry name" value="Glycos_transf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="SSF53448"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0614">Plasmid</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0808">Transferase</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="240"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="273"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="domain" description="Glyco_trans_2-like" evidence="3">
        <location>
            <begin position="12"/>
            <end position="173"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF12827.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="373" mass="40526" checksum="6942DC5D24DEE129" modified="2005-04-26" version="1">MNAASLLPEKISVVVPLFNEEAVLPQFHFRLLRVMDGLASDYEIIYVDDGSRDRSLAVAHNLKAAEARVGIVELSRNFGKEAALTAGLDVASGDAVVIIDADLQDPPELIPSLIEKWREGFDVVYATRTSRDGESFLKKFTAHWFYRLMGKLSDTEIPADTGDFRVMSRRAVAALGQLREQHRFMKGLYAWIGFRQTAVLYRRDARASGTTKFNYRKLLRFAVDGITAFSTVPLKFATSIGLLVAVPAFGMAAFIVGKTLLYGDPVHGYPSMMTALLLLGGLQLVFLGVLGEYVGRLFNEVKRRPVYLVASYTAPIGPGTPTAEPGQVAAATRGGTPPAAIFHAPGCPEGKAPGLPEAPQRDAILTETLAPSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-03-23" modified="2021-06-02" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>D3DXQ8</accession>
    <name>D3DXQ8_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_6466</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ADC45078.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516407.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_6466"/>
    <dbReference type="EnsemblBacteria" id="ADC45078">
        <property type="protein sequence ID" value="ADC45078"/>
        <property type="gene ID" value="Rmet_6466"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6466"/>
    <dbReference type="HOGENOM" id="CLU_2668423_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADC45078.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="75" mass="7789" checksum="EC2432B054E115CB" modified="2010-03-23" version="1">MHQAPPSRLGCDATDAIRQLKTLTEILPTASLLRKAALALLLEANARAQPDALPMALASPPALTPAVPEITGRPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-03-23" modified="2020-10-07" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>D3DXR8</accession>
    <name>D3DXR8_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_6480</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ADC45088.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADC45088">
        <property type="protein sequence ID" value="ADC45088"/>
        <property type="gene ID" value="Rmet_6480"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6480"/>
    <dbReference type="HOGENOM" id="CLU_3315843_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="15"/>
            <end position="35"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADC45088.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="39" mass="4480" checksum="E5895161B81CD5E7" modified="2010-03-23" version="1">MHEDKVFFTKVMDRFLTVLTLVSVGTMGGAMYELLKRSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-03-23" modified="2021-06-02" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>D3DXT5</accession>
    <name>D3DXT5_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_6497</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ADC45105.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_6497"/>
    <dbReference type="EnsemblBacteria" id="ADC45105">
        <property type="protein sequence ID" value="ADC45105"/>
        <property type="gene ID" value="Rmet_6497"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6497"/>
    <dbReference type="HOGENOM" id="CLU_2411056_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="60"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="63"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADC45105.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="92" mass="10660" checksum="FF02E7EA90BA17D5" modified="2010-03-23" version="1">MKAVLIDTLSRGTLTPLDIPVRRPSNKRRLFFYQARLIGIIRYRPIILFPPDRYGTLSPHARRSAVSEKHLHRYGDKKRARSADSQFAISMP</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-03-23" modified="2020-10-07" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>D3DY38</accession>
    <name>D3DY38_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_6609</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ADC45208.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_6609"/>
    <dbReference type="EnsemblBacteria" id="ADC45208">
        <property type="protein sequence ID" value="ADC45208"/>
        <property type="gene ID" value="Rmet_6609"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6609"/>
    <dbReference type="HOGENOM" id="CLU_2790986_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADC45208.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="68" mass="7466" checksum="97D5FFC3516D7B9E" modified="2010-03-23" version="1">MALVGAWACDPDQRQICQPIRLAEGGWNPGDGRPTGNLLSPTEVGYIPHPFELVRLPFRVNPMSGYMA</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-03-23" modified="2020-10-07" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>D3DYA3</accession>
    <name>D3DYA3_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_6688</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ADC45273.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_6688"/>
    <dbReference type="EnsemblBacteria" id="ADC45273">
        <property type="protein sequence ID" value="ADC45273"/>
        <property type="gene ID" value="Rmet_6688"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6688"/>
    <dbReference type="HOGENOM" id="CLU_2828125_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADC45273.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="66" mass="7123" checksum="BF382BA13A98D89B" modified="2010-03-23" version="1">MQIVVVVPGAGVTTVVFCGGLGSLLLNDTQPATQIGSIRIMMSLDMVCPSWMCKRTKEQKNNTRPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-03-23" modified="2020-02-26" version="18" xmlns="http://uniprot.org/uniprot">
    <accession>D3DYG3</accession>
    <name>D3DYG3_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_6754</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ADC45333.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADC45333">
        <property type="protein sequence ID" value="ADC45333"/>
        <property type="gene ID" value="Rmet_6754"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6754"/>
    <dbReference type="HOGENOM" id="CLU_2181747_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADC45333.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="109" mass="11755" checksum="11AAA81EF74F4203" modified="2010-03-23" version="1">MHFVHVALQVGKLEVHGADCIHDPMVEAVGIVANAFSAIAHPFEIIDALTDAALVAGRHDLLRGLAFLVELVHAAHQVLGNRRIGVDGLRCLADRALQMRKTCLDFTGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-10-07" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LA12</accession>
    <name>Q1LA12_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_6155</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="1 2">
        <name>pMOL30</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid pMOL30</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000354">
        <property type="protein sequence ID" value="ABF13014.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF13014">
        <property type="protein sequence ID" value="ABF13014"/>
        <property type="gene ID" value="Rmet_6155"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6155"/>
    <dbReference type="HOGENOM" id="CLU_155885_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid pMOL30"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF13014.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="135" mass="15022" checksum="F928009EC660BC22" modified="2006-05-30" version="1">MFCCGYRLSASTGRSLEAASRCTRTTTENRIYMNLASLIADLESVDNTLTIVAKRPWTANSDARLVSLTDEYRIPSNVLQEGFEYFLEVSIALDEVLGELASQLSPAQRVAAIVYYAENDAYPDWLNTIARSLRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="109" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LAM7</accession>
    <name>Q1LAM7_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">DNA-binding response regulator, LuxR family (Two-component response regulator activity)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Rmet_5940</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="6">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="6">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12799.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5940"/>
    <dbReference type="EnsemblBacteria" id="ABF12799">
        <property type="protein sequence ID" value="ABF12799"/>
        <property type="gene ID" value="Rmet_5940"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5940"/>
    <dbReference type="eggNOG" id="COG2197">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_90_1_4"/>
    <dbReference type="OMA" id="SYKARIM"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06170">
        <property type="entry name" value="LuxR_C_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016032">
        <property type="entry name" value="Sig_transdc_resp-reg_C-effctor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000792">
        <property type="entry name" value="Tscrpt_reg_LuxR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00196">
        <property type="entry name" value="GerE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00038">
        <property type="entry name" value="HTHLUXR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00421">
        <property type="entry name" value="HTH_LUXR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46894">
        <property type="entry name" value="SSF46894"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00622">
        <property type="entry name" value="HTH_LUXR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50043">
        <property type="entry name" value="HTH_LUXR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="5" id="KW-0614">Plasmid</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Response regulatory" evidence="4">
        <location>
            <begin position="13"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="domain" description="HTH luxR-type" evidence="3">
        <location>
            <begin position="152"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="2">
        <location>
            <position position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABF12799.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="220" mass="24121" checksum="CE8AB60ACDEF6D30" modified="2006-05-30" version="1">MVGLPPRQQGMASILIVDDHPALRLALRTQLSQLLGVTTLLEADNGQGALNLVRQHRPDLVLLDLDIPRMSGLDAVPRIRALHPAVRILVLSAQDPIPFAARAYHAGAQGFVSKTQNMDAILRCVESVLGGFTVFPNNNPRDNPRESRVVGDSEGLSKLTDKEVEILRMLTRGMSNKAIGKALFISNKTVSSYKTRVMKKLAADSLVDLIDFARRCRLIP</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="65" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LAR6</accession>
    <name>Q1LAR6_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_5901</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12760.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518108.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5901"/>
    <dbReference type="EnsemblBacteria" id="ABF12760">
        <property type="protein sequence ID" value="ABF12760"/>
        <property type="gene ID" value="Rmet_5901"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5901"/>
    <dbReference type="eggNOG" id="COG0628">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_798794_0_0_4"/>
    <dbReference type="OMA" id="HEAENPG"/>
    <dbReference type="OrthoDB" id="1354423at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="44"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="90"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="180"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="226"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="260"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="284"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="326"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF12760.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="364" mass="39688" checksum="73E59AA886682154" modified="2006-05-30" version="1">MPQLDNELDTEQTEDRAGTGSTEQPDQTRPLTPAAWFSVKTVSYVLTAGALLLVMHANLVSALLAGLLLYSLVDVLAPRLMRLHSRRDAIAVAILSTIILLVLTGSIWALIRFISGDGNTLDRLLDHSADIIDRSRAQLPAWLSDYLPNGVDELATTLISALREHAQMAQRLGADILRTLVHILVGLIIGAMIALYRAISRPNRRPLALALVDRARTLQTAFSQFIFAQIQISLVNTILTAIYLLVLLPLFHQHLPLSKTLVAITFIAGLLPVLGNLISNTAIVIASLSVSLPIAVVSLLFLVLIHKLEYFLNARIIGARIHAAAWELLTVMLVMETLYGIPGVIAGPIFYAYVKRELSTAGLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="62" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LAU1</accession>
    <name>Q1LAU1_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_5876</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12735.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008643456.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5876"/>
    <dbReference type="EnsemblBacteria" id="ABF12735">
        <property type="protein sequence ID" value="ABF12735"/>
        <property type="gene ID" value="Rmet_5876"/>
    </dbReference>
    <dbReference type="GeneID" id="60823673"/>
    <dbReference type="KEGG" id="rme:Rmet_5876"/>
    <dbReference type="eggNOG" id="ENOG50303WG">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_118626_1_0_4"/>
    <dbReference type="OMA" id="TGLHEAN"/>
    <dbReference type="OrthoDB" id="1911841at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="62"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="90"/>
            <end position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF12735.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="170" mass="18112" checksum="5C8CDC3CFF152102" modified="2006-05-30" version="1">MRKRIFWLLPDLASARRTMDDLLLARIENSHIHFVGRDGADMTGLHEANLFQTSDIVHAAEMGLMVGGGVGIVAGVVVAMFPIVSDSPQWGLVGVLAVLGAVFGAWAASMIGSSAPNSRLRSFEKDIEAGKLLLMVDVPRGRVEEVEALLQKAHPEASFAGLDPAVPAFP</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="96" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LAZ2</accession>
    <name>Q1LAZ2_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">3-oxoacyl-(Acyl-carrier-protein) reductase</fullName>
            <ecNumber evidence="2">1.1.1.100</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_5825</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EC" id="1.1.1.100" evidence="2"/>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12684.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5825"/>
    <dbReference type="EnsemblBacteria" id="ABF12684">
        <property type="protein sequence ID" value="ABF12684"/>
        <property type="gene ID" value="Rmet_5825"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5825"/>
    <dbReference type="eggNOG" id="COG1028">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010194_1_3_4"/>
    <dbReference type="OMA" id="VGWVEPV"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004316">
        <property type="term" value="F:3-oxoacyl-[acyl-carrier-protein] reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00061">
        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF12684.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="298" mass="31455" checksum="287D9DD302105209" modified="2006-05-30" version="1">MKARRVSTHSTENKTTRTKETRSMSKLTNKVAIVSGSGRGIGREIALKLASEGAAVVVNDLDAEPAEAVVREITTMGGRAVACVGSVTEADFGTRFVGTALQTFGGLDIIVNNAGYTWDNVIQKMTDDQWSAIMDVHVTAPFRILRAAADYFRETSKQEAEQGREVFRKVVNISSASGVIGNAGQANYSAGKAAINGLTKALAKEWGRYKVNVNSVAFGLIKTRLTEAVAGEGHATIDIAGNEIKVGVNPQLMKNIEATIPLGRGGTPAEAAGSVYLLCIPESNYITGQVLVVGGGRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="53" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LB07</accession>
    <name>Q1LB07_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_5810</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12669.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5810"/>
    <dbReference type="EnsemblBacteria" id="ABF12669">
        <property type="protein sequence ID" value="ABF12669"/>
        <property type="gene ID" value="Rmet_5810"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5810"/>
    <dbReference type="HOGENOM" id="CLU_2344604_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="65"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="73"/>
            <end position="97"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF12669.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="97" mass="10049" checksum="83C321556EB196B4" modified="2006-05-30" version="1">MRAVKHRADTPARVDQGTITGRVIHSPGYQEAIVIRTMLGRSHVVRDIGLTLCLTFCLGTLAGCDRNQAPGPGPKPISSNGSQAAATSASSPAGTPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="99" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LB76</accession>
    <name>Q1LB76_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Serine protease, subtilase family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Rmet_5741</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="8">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="8">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12600.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011520142.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5741"/>
    <dbReference type="MEROPS" id="S08.107"/>
    <dbReference type="EnsemblBacteria" id="ABF12600">
        <property type="protein sequence ID" value="ABF12600"/>
        <property type="gene ID" value="Rmet_5741"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5741"/>
    <dbReference type="eggNOG" id="COG1404">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011263_8_1_4"/>
    <dbReference type="OMA" id="WTTAGQC"/>
    <dbReference type="OrthoDB" id="923655at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021206">
        <property type="entry name" value="MprA_tail"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000209">
        <property type="entry name" value="Peptidase_S8/S53_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036852">
        <property type="entry name" value="Peptidase_S8/S53_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022398">
        <property type="entry name" value="Peptidase_S8_His-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023828">
        <property type="entry name" value="Peptidase_S8_Ser-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015500">
        <property type="entry name" value="Peptidase_S8_subtilisin-rel"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00082">
        <property type="entry name" value="Peptidase_S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00723">
        <property type="entry name" value="SUBTILISIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52743">
        <property type="entry name" value="SSF52743"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03867">
        <property type="entry name" value="MprA_tail"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00137">
        <property type="entry name" value="SUBTILASE_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00138">
        <property type="entry name" value="SUBTILASE_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0614">Plasmid</keyword>
    <keyword evidence="1 7" id="KW-0645">Protease</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0720">Serine protease</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="529"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase_S8" evidence="6">
        <location>
            <begin position="163"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="195"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="490"/>
            <end position="524"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="492"/>
            <end position="524"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABF12600.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="564" mass="56322" checksum="31A9383D94B63BC7" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LBD2</accession>
    <name>Q1LBD2_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Type II secretion system protein H</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">General secretion pathway protein H</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="primary">fimT</name>
        <name evidence="13" type="ordered locus">Rmet_5685</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="14">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="14">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell inner membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12544.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011520086.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5685"/>
    <dbReference type="EnsemblBacteria" id="ABF12544">
        <property type="protein sequence ID" value="ABF12544"/>
        <property type="gene ID" value="Rmet_5685"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5685"/>
    <dbReference type="eggNOG" id="COG4970">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_084761_5_3_4"/>
    <dbReference type="OMA" id="RRCIRIA"/>
    <dbReference type="OrthoDB" id="1621805at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015627">
        <property type="term" value="C:type II protein secretion system complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015628">
        <property type="term" value="P:protein secretion by the type II secretion system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012902">
        <property type="entry name" value="N_methyl_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045584">
        <property type="entry name" value="Pilin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022346">
        <property type="entry name" value="T2SS_GspH"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12019">
        <property type="entry name" value="GspH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07963">
        <property type="entry name" value="N_methyl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54523">
        <property type="entry name" value="SSF54523"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02532">
        <property type="entry name" value="IV_pilin_GFxxxE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00409">
        <property type="entry name" value="PROKAR_NTER_METHYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0488">Methylation</keyword>
    <keyword evidence="13" id="KW-0614">Plasmid</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 11 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="20"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="domain" description="GspH" evidence="12">
        <location>
            <begin position="52"/>
            <end position="154"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004377"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00021549"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00030775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF12019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ABF12544.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="164" mass="16416" checksum="598E0FF9E2B773BB" modified="2006-05-30" version="1">MPNFSPTLPATQRAAGFTLIELMITVIVLVIVVSMAAPAMSQLISSQRTRNAAADLASALTLTRSEAVNRSATTTMTASGTGGSWTGGWAVAAGTARIRGYGPYDGITITASGGSSLAIGNDGRPTAGTQTFQVAPSTNATSATTVCVQVGGTGRVTTVNGTCT</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-10-07" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LBF1</accession>
    <name>Q1LBF1_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Transcriptional regulator, LysR family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_5666</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the LysR transcriptional regulatory family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12525.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5666"/>
    <dbReference type="EnsemblBacteria" id="ABF12525">
        <property type="protein sequence ID" value="ABF12525"/>
        <property type="gene ID" value="Rmet_5666"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5666"/>
    <dbReference type="eggNOG" id="COG0583">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039613_2_0_4"/>
    <dbReference type="OMA" id="TWVAMHE"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005119">
        <property type="entry name" value="LysR_subst-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000847">
        <property type="entry name" value="Tscrpt_reg_HTH_LysR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00126">
        <property type="entry name" value="HTH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03466">
        <property type="entry name" value="LysR_substrate"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00039">
        <property type="entry name" value="HTHLYSR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50931">
        <property type="entry name" value="HTH_LYSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH lysR-type" evidence="2">
        <location>
            <begin position="9"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF12525.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="306" mass="33182" checksum="9F2D1F795EB90549" modified="2006-05-30" version="1">MHTMTRNEPSWEWYRTFLQVLEVGSLSAAGRALGITQPTVGRHIDSLESALGLKLFTRSFDGFAPTEAAHELKPYAASIAATAAALRRVASSHGAGVRGTVRLTASEVIGAEVLPPILAALRDAHPELVVELVLSNQPDDLLRREADIAIRMFRPVQGALIARHVGGIEIGLHAHSRYLQAHGVPGSTSELSRHALIGFDQENDFIRRLQSKFKPFWRDSLAFRSDSDLAQLGAIRAGFGIGVCQAALAARDPQLVRVLPEQFSVTLDTWVAMHEDLRDNTRCAVTFAALVNGLDAYIKGVPGRGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="97" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LBR7</accession>
    <name>Q1LBR7_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Putative transcriptional regulator, GntR family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Rmet_5550</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="6">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="6">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12409.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519956.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5550"/>
    <dbReference type="EnsemblBacteria" id="ABF12409">
        <property type="protein sequence ID" value="ABF12409"/>
        <property type="gene ID" value="Rmet_5550"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5550"/>
    <dbReference type="eggNOG" id="COG2186">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017584_9_3_4"/>
    <dbReference type="OMA" id="GSYQAMW"/>
    <dbReference type="OrthoDB" id="1875458at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07377">
        <property type="entry name" value="WHTH_GntR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.530">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011711">
        <property type="entry name" value="GntR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008920">
        <property type="entry name" value="TF_FadR/GntR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000524">
        <property type="entry name" value="Tscrpt_reg_HTH_GntR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07729">
        <property type="entry name" value="FCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00392">
        <property type="entry name" value="GntR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00035">
        <property type="entry name" value="HTHGNTR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00895">
        <property type="entry name" value="FCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00345">
        <property type="entry name" value="HTH_GNTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48008">
        <property type="entry name" value="SSF48008"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50949">
        <property type="entry name" value="HTH_GNTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0614">Plasmid</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH gntR-type" evidence="4">
        <location>
            <begin position="13"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABF12409.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="240" mass="26886" checksum="26409340DD60DE93" modified="2006-05-30" version="1">MTYAQEATAEPRASAGTNVIAGILAYLQDRRLQPGDRLPSERDLAERLGVGRNAVREALATLVTLRVVESRPNSGVYLRHMSRESSFEALVLLTDIGATPTPTEVTETMEVRAHLELLAVSLACQRRTDEDLARMEAILARTDQTLEAGGNIAEMDTDFHIAVVDAAHNSVLVRTLNAFYRFTARRREVLFADHTQGLASAREHRQMLEHISNRDISKAERLILQHMDRATTYWSSYLET</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LBU7</accession>
    <name>Q1LBU7_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Acyl dehydratase (MaoC-like domain)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_5520</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12379.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519923.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5520"/>
    <dbReference type="EnsemblBacteria" id="ABF12379">
        <property type="protein sequence ID" value="ABF12379"/>
        <property type="gene ID" value="Rmet_5520"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5520"/>
    <dbReference type="eggNOG" id="COG2030">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_040078_1_0_4"/>
    <dbReference type="OMA" id="PRPIMHG"/>
    <dbReference type="OrthoDB" id="942354at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029069">
        <property type="entry name" value="HotDog_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039569">
        <property type="entry name" value="MaoC-like_dehydrat_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002539">
        <property type="entry name" value="MaoC-like_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13452">
        <property type="entry name" value="MaoC_dehydrat_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01575">
        <property type="entry name" value="MaoC_dehydratas"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54637">
        <property type="entry name" value="SSF54637"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MaoC_dehydrat_N" evidence="2">
        <location>
            <begin position="51"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="domain" description="MaoC-like" evidence="1">
        <location>
            <begin position="166"/>
            <end position="277"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01575"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF12379.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="291" mass="31356" checksum="DED0959CF4306DF4" modified="2006-05-30" version="1">MIDYQKTKAWQSGDVIHNYTEKDSILYALGLGIGSDPLDESQLRFVYEKNLVTLPTMAAVIASPGSWMRDRKELGIDFLKLVHGEQCVTVHKVLPSAGSLIGRSRVVRIVDKGEGKGAVLHVEKNLYDAKTDEHVATAEQVLFLRGDGGFSQNGGGDEPAAAAPPTPEGSPDIRVELPTRADAALLYRLSGDTNPLHIDPAVASKAGFARPILHGLATYGVACHGIVKAFCDYDPSRITSIRARLTSPVYPGETIVLECWKVGVNEIAFRGRLKERDVIALANGRATLSAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-10-07" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LCC5</accession>
    <name>Q1LCC5_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_5342</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12201.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF12201">
        <property type="protein sequence ID" value="ABF12201"/>
        <property type="gene ID" value="Rmet_5342"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5342"/>
    <dbReference type="HOGENOM" id="CLU_2275141_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF12201.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="102" mass="11588" checksum="6E23F0FD2939DB65" modified="2006-05-30" version="1">MSSVYPPKVKFDRYLLLRNLQSGDGSTRTQSVARARRFFNRKKLKARKSSVRIDAVMLQGNRNTTRKAGERKGLAEGPATPCTLDARRKNFLKSMFNDSLLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="110" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LCE0</accession>
    <name>Q1LCE0_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Histidine kinase</fullName>
            <ecNumber evidence="2">2.7.13.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">zneS</name>
        <name evidence="10" type="ordered locus">Rmet_5327</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="11">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="11">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.</text>
            <dbReference type="EC" id="2.7.13.3"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.7.13.3" evidence="2"/>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12186.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519729.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5327"/>
    <dbReference type="EnsemblBacteria" id="ABF12186">
        <property type="protein sequence ID" value="ABF12186"/>
        <property type="gene ID" value="Rmet_5327"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5327"/>
    <dbReference type="eggNOG" id="COG2205">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_89_37_4"/>
    <dbReference type="OMA" id="QILAQIH"/>
    <dbReference type="OrthoDB" id="692375at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00082">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003660">
        <property type="entry name" value="HAMP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003661">
        <property type="entry name" value="HisK_dim/P"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036097">
        <property type="entry name" value="HisK_dim/P_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004358">
        <property type="entry name" value="Sig_transdc_His_kin-like_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00512">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00344">
        <property type="entry name" value="BCTRLSENSOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00388">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47384">
        <property type="entry name" value="SSF47384"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="SSF55874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50885">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 10" id="KW-0418">Kinase</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="10" id="KW-0614">Plasmid</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="21"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="154"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="domain" description="HAMP" evidence="9">
        <location>
            <begin position="177"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="8">
        <location>
            <begin position="237"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="457"/>
            <end position="480"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ABF12186.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="480" mass="52246" checksum="DFFAEC254F01CE38" modified="2006-05-30" version="1">MAGAEGRLKHAGPKHSVQQRLSLWVSLAILAVALIGGIFSFASAFREANIMQDDLLRQVSMLFDAQHLPAPHLADAGRLPHSDEEARVIVQTLSPGGDSARQILALPASAPDGLGTFEVDHESYRVLIRTLPTGQRIAVAQETGMRDEIARNSAQVTVLPFLVLVPVLLLIVAYLVRQMFRPISTLSTDIDRRGEHELYPLPEDELPAEVRPFVVAINRLLARIAQAMEMQRRFVADAAHELRSPMTALSLQAERLERTEMPEAARTRLATLRRGIERSRALLEQLLSLARAQSATAAPAPAESISVRHAFRRVLEDLMPLADSRHIDIGAVGDTDAQVPIQEVELITLLKNLVDNAIRYTPKHGRVDLSVETSPNAVAMIVTDSGPGISTEERSRIFDPFYRIPGNDAPGSGLGLSIVRVIAERVGATVEIDYADAAARHGTRVTVRVPCGDNCGGNYGTNESGVRRQHAAATTDQPLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LCE6</accession>
    <name>Q1LCE6_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Heavy metal cation tricomponent efflux outer membrane porin ZniC (CzcC-like)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">zniC</name>
        <name evidence="3" type="ordered locus">Rmet_5321</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the outer membrane factor (OMF) (TC 1.B.17) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12180.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519724.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5321"/>
    <dbReference type="EnsemblBacteria" id="ABF12180">
        <property type="protein sequence ID" value="ABF12180"/>
        <property type="gene ID" value="Rmet_5321"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5321"/>
    <dbReference type="eggNOG" id="COG1538">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012817_13_0_4"/>
    <dbReference type="OMA" id="NMYPQLT"/>
    <dbReference type="OrthoDB" id="1619477at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015562">
        <property type="term" value="F:efflux transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003423">
        <property type="entry name" value="OMP_efflux"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010131">
        <property type="entry name" value="RND_efflux_OM_lipoprot_NodT"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02321">
        <property type="entry name" value="OEP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01845">
        <property type="entry name" value="outer_NodT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0564">Palmitate</keyword>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1134">Transmembrane beta strand</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5001442047" evidence="2">
        <location>
            <begin position="34"/>
            <end position="518"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU362097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF12180.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="518" mass="55300" checksum="678B0C939ED7DC36" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="66" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LCN3</accession>
    <name>Q1LCN3_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">MPAB_Lcp_cat domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_5234</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12093.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5234"/>
    <dbReference type="EnsemblBacteria" id="ABF12093">
        <property type="protein sequence ID" value="ABF12093"/>
        <property type="gene ID" value="Rmet_5234"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5234"/>
    <dbReference type="eggNOG" id="COG3662">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_059206_0_1_4"/>
    <dbReference type="OMA" id="HGDFTSM"/>
    <dbReference type="OrthoDB" id="1395053at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018713">
        <property type="entry name" value="MPAB/Lcp_cat_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09995">
        <property type="entry name" value="MPAB_Lcp_cat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MPAB_Lcp_cat" evidence="2">
        <location>
            <begin position="61"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="10"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF09995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF12093.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="310" mass="33417" checksum="16840F9BEAE07722" modified="2006-05-30" version="1">MPPGLPRSSAPSTPSAGPLQRLRNQAGAQVRALTRSNSGLTLDYDNPPGDPGLFGPDAVCWRVHSDFPAMLAGGVSALLLQALHPLALAGVWDHSTFRTDMQGRLGRTAQFIAGTTYGSHADAMALIERVRRIHACIKGTAPDGQPYAADDPALLTWVHVAEVSSFLTGYLRYVGPLSAAEQDRYFEEEARVAALLGAPDVPRSRAEIDAYLEAQRPLLVNSERTREVVQLVMTMPVANPLLIPAVRTMADAGVALLPPWARQMLGLHRPSLRRSLALTGMRALAPTLRWALGPGLAARARRRVANLPKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="95" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LCU8</accession>
    <name>Q1LCU8_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Transcriptional regulator, LysR family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_5169</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the LysR transcriptional regulatory family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12028.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519573.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5169"/>
    <dbReference type="EnsemblBacteria" id="ABF12028">
        <property type="protein sequence ID" value="ABF12028"/>
        <property type="gene ID" value="Rmet_5169"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5169"/>
    <dbReference type="eggNOG" id="COG0583">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039613_20_4_4"/>
    <dbReference type="OMA" id="RFWREAS"/>
    <dbReference type="OrthoDB" id="1661500at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005119">
        <property type="entry name" value="LysR_subst-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000847">
        <property type="entry name" value="Tscrpt_reg_HTH_LysR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00126">
        <property type="entry name" value="HTH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03466">
        <property type="entry name" value="LysR_substrate"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50931">
        <property type="entry name" value="HTH_LYSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH lysR-type" evidence="2">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF12028.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="140" mass="15456" checksum="CFCFBC585255C098" modified="2006-05-30" version="1">MSQPGVSRAIARREEQLGLRLFDRTTRSIPMTDEGRSFYEQVIPLIATLDAITASASGSAKTINGRLRVNIDPFLSRLMLGSRLGAFLQRHPDLQLELITRDELGDMITDGFDVAIRFGGHARRYASSYPSAACITEDLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LCX7</accession>
    <name>Q1LCX7_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative AMP-dependent synthetase and ligase / putative o-succinylbenzoate-CoA ligase</fullName>
            <ecNumber evidence="3">6.2.1.-</ecNumber>
            <ecNumber evidence="3">6.2.1.26</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_5140</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EC" id="6.2.1.-" evidence="3"/>
    <dbReference type="EC" id="6.2.1.26" evidence="3"/>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11999.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519546.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5140"/>
    <dbReference type="EnsemblBacteria" id="ABF11999">
        <property type="protein sequence ID" value="ABF11999"/>
        <property type="gene ID" value="Rmet_5140"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5140"/>
    <dbReference type="eggNOG" id="COG0318">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000022_59_0_4"/>
    <dbReference type="OMA" id="FHVNAIC"/>
    <dbReference type="OrthoDB" id="377638at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008756">
        <property type="term" value="F:o-succinylbenzoate-CoA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025110">
        <property type="entry name" value="AMP-bd_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045851">
        <property type="entry name" value="AMP-bd_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_Synth/Lig"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13193">
        <property type="entry name" value="AMP-binding_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0436">Ligase</keyword>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AMP-binding" evidence="1">
        <location>
            <begin position="15"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="domain" description="AMP-binding_C" evidence="2">
        <location>
            <begin position="431"/>
            <end position="506"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF11999.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="525" mass="57207" checksum="C824BDB66E54DD13" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="65" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LCX9</accession>
    <name>Q1LCX9_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_5138</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11997.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519544.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5138"/>
    <dbReference type="EnsemblBacteria" id="ABF11997">
        <property type="protein sequence ID" value="ABF11997"/>
        <property type="gene ID" value="Rmet_5138"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5138"/>
    <dbReference type="eggNOG" id="COG1328">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_195213_2_1_4"/>
    <dbReference type="OMA" id="RCEIWTR"/>
    <dbReference type="OrthoDB" id="2071943at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008998">
        <property type="term" value="F:ribonucleoside-triphosphate reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012833">
        <property type="entry name" value="NrdD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13597">
        <property type="entry name" value="NRDD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF11997.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="63" mass="7207" checksum="B4250B614A5ABF3A" modified="2006-05-30" version="1">MTDLAVARHFSIEPALALKDDERQPCEVWTRVMGYHRPVASFNTGKKGEHAERTFFDEIRCAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LDC6</accession>
    <name>Q1LDC6_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Lipoprotein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Rmet_4988</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="7">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="7">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the nlpA lipoprotein family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11850.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519398.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4988"/>
    <dbReference type="EnsemblBacteria" id="ABF11850">
        <property type="protein sequence ID" value="ABF11850"/>
        <property type="gene ID" value="Rmet_4988"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4988"/>
    <dbReference type="eggNOG" id="COG1464">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_067080_0_1_4"/>
    <dbReference type="OMA" id="PHTIVFG"/>
    <dbReference type="OrthoDB" id="1230456at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004872">
        <property type="entry name" value="Lipoprotein_NlpA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30429">
        <property type="entry name" value="PTHR30429"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03180">
        <property type="entry name" value="Lipoprotein_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002854">
        <property type="entry name" value="MetQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0614">Plasmid</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193372" description="Lipoprotein" evidence="5">
        <location>
            <begin position="25"/>
            <end position="270"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRNR" id="PIRNR002854"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABF11850.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="270" mass="29593" checksum="62E0EEC7F5F03C6D" modified="2006-05-30" version="1">MKLGKGLRLFTALLAVTVMQAAGAADPDKKEIRFGATAGPYADQIRYGVKPILEQQGYKVTIIEFSDYVQPNLALADGAIDANAFQHVVYLKKFSSDRKLALSEIVQVPTAPIGIYSRKHKSLAEVKTGATVSLPNDPTNLARAIAILQQIGWVTLKPGTDAVRASERDIDGNPHKLKLIQLEAAQLPRSLDDVDYAFVNGNFALAAGLKLTTALALEKIPDYYMNLVAVRTSDLNRPYVADIREAYRSQAFKQVTQQRFAGFVAPAYQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="61" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LDJ2</accession>
    <name>Q1LDJ2_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF2249 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_4922</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11784.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519345.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4922"/>
    <dbReference type="EnsemblBacteria" id="ABF11784">
        <property type="protein sequence ID" value="ABF11784"/>
        <property type="gene ID" value="Rmet_4922"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4922"/>
    <dbReference type="eggNOG" id="COG4309">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_146484_3_0_4"/>
    <dbReference type="OMA" id="EGPDVWR"/>
    <dbReference type="OrthoDB" id="1932815at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018720">
        <property type="entry name" value="DUF2249"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10006">
        <property type="entry name" value="DUF2249"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF2249" evidence="1">
        <location>
            <begin position="10"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF10006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF11784.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="95" mass="10437" checksum="4D40E38187876C56" modified="2006-05-30" version="1">MSQSTSNPQLDLRTIPPRDRHPLIFSTFTGLEVGQALELVNDHDPRPLQMQFQIERPGQFAWDYLEQGPATWRVAIKKVASAAKVGQCCGGCGGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="62" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LDK6</accession>
    <name>Q1LDK6_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Protein involved in acute metal response (CopQ/CzcJ/MmrQ/MmtQ/MmmQ-like)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">mmsQ</name>
        <name evidence="3" type="ordered locus">Rmet_4908</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11770.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519329.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4908"/>
    <dbReference type="EnsemblBacteria" id="ABF11770">
        <property type="protein sequence ID" value="ABF11770"/>
        <property type="gene ID" value="Rmet_4908"/>
    </dbReference>
    <dbReference type="GeneID" id="60823837"/>
    <dbReference type="KEGG" id="rme:Rmet_4908"/>
    <dbReference type="HOGENOM" id="CLU_1400456_0_0_4"/>
    <dbReference type="OMA" id="GYTFRTG"/>
    <dbReference type="OrthoDB" id="1531407at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193373" evidence="2">
        <location>
            <begin position="24"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="134"/>
            <end position="165"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF11770.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="165" mass="17186" checksum="8E5A866037BD02FA" modified="2006-05-30" version="1">MNAKTILTAAALAAAFVTGAAQAAPAAQKAGEVYGYSYRVNDQRSAFTDGARVGKTDPYTDGAKVGKADPFTDGAKVGKFDPYTDGARIVAGLDRTGVSSEPARKVDPYLDGAKVGKFDPYTDGARMVAGLDRTGVSSEPARKVDPYYDGARTGKFDPYTEGANA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="111" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LDM6</accession>
    <name>Q1LDM6_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Dihydrolipoamide dehydrogenase</fullName>
            <ecNumber evidence="7">1.8.1.4</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">lpdA</name>
        <name evidence="7" type="ordered locus">Rmet_4888</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="8">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="8">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
        <text evidence="3">Binds 1 FAD per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family.</text>
    </comment>
    <dbReference type="EC" id="1.8.1.4" evidence="7"/>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11750.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519309.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4888"/>
    <dbReference type="EnsemblBacteria" id="ABF11750">
        <property type="protein sequence ID" value="ABF11750"/>
        <property type="gene ID" value="Rmet_4888"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4888"/>
    <dbReference type="eggNOG" id="COG1249">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016755_0_3_4"/>
    <dbReference type="OMA" id="VEHIWAM"/>
    <dbReference type="OrthoDB" id="267896at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004148">
        <property type="term" value="F:dihydrolipoyl dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045454">
        <property type="term" value="P:cell redox homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.990">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.390.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023753">
        <property type="entry name" value="FAD/NAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016156">
        <property type="entry name" value="FAD/NAD-linked_Rdtase_dimer_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036324">
        <property type="entry name" value="Mn/Fe_SOD_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001100">
        <property type="entry name" value="Pyr_nuc-diS_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004099">
        <property type="entry name" value="Pyr_nucl-diS_OxRdtase_dimer"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07992">
        <property type="entry name" value="Pyr_redox_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02852">
        <property type="entry name" value="Pyr_redox_dim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000350">
        <property type="entry name" value="Mercury_reductase_MerA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="SSF51905"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55424">
        <property type="entry name" value="SSF55424"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0274">FAD</keyword>
    <keyword evidence="3" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="3" id="KW-0520">NAD</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-0614">Plasmid</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pyr_redox_2" evidence="6">
        <location>
            <begin position="19"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="domain" description="Pyr_redox_dim" evidence="5">
        <location>
            <begin position="361"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="2">
        <location>
            <position position="455"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="64"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="154"/>
            <end position="156"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="191"/>
            <end position="198"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="281"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="323"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="disulfide bond" description="Redox-active" evidence="4">
        <location>
            <begin position="55"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR000350-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR000350-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR000350-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF02852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF07992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABF11750.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="480" mass="51343" checksum="0CBD9F60FF2D140B" modified="2006-05-30" version="1">MNTNQNAQTAQTVQTVQTDVAIIGAGTAGLAAYRAAIAAGKRAVIIEGGAYGTTCARVGCMPSKLLIAAAEAAHEARHTAAFGVHVDGPVRIDGREVMQRVRSERDRFVGFVVRGVESIPQDDRLIGRARFIDNTILQVDDHTVVRASRVVIATGSRPAVPPSFGVFGDRLIVNDDVFSWEDLPQSVAVFGPGVIGLELGQALSRLGVRVRVFGVGGGVGPLSDPEIRAYAAKSFNQEFSLLADARVLEMRREGDDAVITFEDADGQTVTERFAYVLAATGRRPNVDTLALGNTALELDGRGVPTFDPVTLQCGESPIFIAGDANNVLPLLHEAADEGKIAGENAALWPKVGPLTRRAPIAVVFSDPQIAMVGKRFAELTPGSFVTGEVSFEDQGRSRVMLKNRGLMHVYADRATGRFIGAEWIGPRAENIAHLLAWSYQQGLTIAAMLAMPFYHPVVEEGLRTALRDAQAKLAQLQEAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="96" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LDN6</accession>
    <name>Q1LDN6_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Chloromuconate cycloisomerase</fullName>
            <ecNumber evidence="5">5.5.1.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">catB</name>
        <name evidence="9" type="ordered locus">Rmet_4878</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="10">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="10">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(2R)-2-chloro-2,5-dihydro-5-oxofuran-2-acetate = 3-chloro-cis,cis-muconate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:11032"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17589"/>
            <dbReference type="ChEBI" id="CHEBI:85538"/>
            <dbReference type="EC" id="5.5.1.7"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Aromatic compound metabolism; 3-chlorocatechol degradation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the mandelate racemase/muconate lactonizing enzyme family.</text>
    </comment>
    <dbReference type="EC" id="5.5.1.7" evidence="5"/>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11740.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519299.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4878"/>
    <dbReference type="EnsemblBacteria" id="ABF11740">
        <property type="protein sequence ID" value="ABF11740"/>
        <property type="gene ID" value="Rmet_4878"/>
    </dbReference>
    <dbReference type="GeneID" id="60823868"/>
    <dbReference type="KEGG" id="rme:Rmet_4878"/>
    <dbReference type="eggNOG" id="COG4948">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030273_4_5_4"/>
    <dbReference type="OMA" id="GRQDEWF"/>
    <dbReference type="OrthoDB" id="951991at2"/>
    <dbReference type="UniPathway" id="UPA00083"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018850">
        <property type="term" value="F:chloromuconate cycloisomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030145">
        <property type="term" value="F:manganese ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018849">
        <property type="term" value="F:muconate cycloisomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019439">
        <property type="term" value="P:aromatic compound catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009063">
        <property type="term" value="P:cellular amino acid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03318">
        <property type="entry name" value="MLE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.390.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013370">
        <property type="entry name" value="Chloromuconate_cycloisomerase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036849">
        <property type="entry name" value="Enolase-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029017">
        <property type="entry name" value="Enolase-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029065">
        <property type="entry name" value="Enolase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018110">
        <property type="entry name" value="Mandel_Rmase/mucon_lact_enz_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013342">
        <property type="entry name" value="Mandelate_racemase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013341">
        <property type="entry name" value="Mandelate_racemase_N_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13378">
        <property type="entry name" value="MR_MLE_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02746">
        <property type="entry name" value="MR_MLE_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01258">
        <property type="entry name" value="(chloro)muconate_cycloisomeras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00922">
        <property type="entry name" value="MR_MLE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51604">
        <property type="entry name" value="SSF51604"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54826">
        <property type="entry name" value="SSF54826"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02534">
        <property type="entry name" value="mucon_cyclo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00909">
        <property type="entry name" value="MR_MLE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0058">Aromatic hydrocarbons catabolism</keyword>
    <keyword evidence="9" id="KW-0413">Isomerase</keyword>
    <keyword evidence="7" id="KW-0464">Manganese</keyword>
    <keyword evidence="9" id="KW-0614">Plasmid</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MR_MLE" evidence="8">
        <location>
            <begin position="147"/>
            <end position="244"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000453"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004674"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022797"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ABF11740.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="374" mass="39820" checksum="F3B7E294BD6E5CDC" modified="2006-05-30" version="1">MTATISSIEAILVDLPTIRAHQLAMATMQQQTLVIVRLRCSDGIEGIGEATTIGGLSYGDESPEGIKLTIDTYLAPALVGQDATNVHAAMARLNKVARGNRFAKSALESAMLDAQGKRLDVPLATLLGGAVRDTLPVLWTLASGDTARDIEEAERFLEERRHNTFKLKIGRRSVREDVAHVSAIKRALGDRARVTVDVNQAWNEADAATGIAMLEAAGIDLIEQPTPREQRTALARLAARFVVPIMADEAVTGPEDAMELARLGAADVFALKIAKSGGIFGMLRTAAVGDAAGIALYGGTMLEGSVGTIAAAHGFCTLPQLAWGTELFGPLLVKDDIVVERPVFKDFSLHLPQGPGLGLAIDEDKLAHYRRKSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LDU7</accession>
    <name>Q1LDU7_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Nitrate transporter major facilitator superfamily</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">narK</name>
        <name evidence="9" type="ordered locus">Rmet_4817</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="10">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="10">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily. Nitrate/nitrite porter (TC 2.A.1.8) family.</text>
    </comment>
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        <property type="protein sequence ID" value="ABF11679.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_007974.2"/>
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        <property type="taxonomic scope" value="Bacteria"/>
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    <dbReference type="HOGENOM" id="CLU_024204_4_1_4"/>
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    <dbReference type="Proteomes" id="UP000002429">
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    </dbReference>
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        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="term" value="F:nitrate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042128">
        <property type="term" value="P:nitrate assimilation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044772">
        <property type="entry name" value="NO3_transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23515">
        <property type="entry name" value="PTHR23515"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="SSF103473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0534">Nitrate assimilation</keyword>
    <keyword evidence="9" id="KW-0614">Plasmid</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="21"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="56"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="86"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="110"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="150"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="178"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="241"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="270"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="312"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="338"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="367"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="402"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="domain" description="MFS" evidence="8">
        <location>
            <begin position="20"/>
            <end position="425"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ABF11679.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="437" mass="45960" checksum="253F1B735E3065AB" modified="2006-05-30" version="1">MNGKATRIDLLSFKTPQMRAFHLTWMAFFVCFFAWFACAPLMPVLKGEFGLTPNQIANINIAAVAVTILVRLVIGPLCDRFGPRKTYTGLLAIGALPVLGVAFAQNYETFLFFRLLIGAVGASFVITQYHTSVMFAPNVVGTANAASAGWGNAGGGAAQGLMPLLLATILFLGVDHTMGWRVALLVPGILMLVMAVVYWRFTQDCPEGNFSELRARGIAVSGGKGGGGWASFRAASANYRVWMLFITYGACFGVEIFIHNVAATFYVDRFGLSLSAAGMAAASFGLLALFARALGGYVSDKLAMRRGLDARSLILFVFILGEGLGLLGFAHASTATTAVAAMLVFGLFTHMACGATYALVPFIDRKALGGVAGIIGAGGNVGAVAAGFLMKGLGDLQQTLTWLGVAATLAALCAIAVRFSAEHKAQEQALYDSALAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LF09</accession>
    <name>Q1LF09_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Transmembrane protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_4402</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11267.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4402"/>
    <dbReference type="EnsemblBacteria" id="ABF11267">
        <property type="protein sequence ID" value="ABF11267"/>
        <property type="gene ID" value="Rmet_4402"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4402"/>
    <dbReference type="eggNOG" id="COG0697">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033863_10_0_4"/>
    <dbReference type="OMA" id="FALEPMH"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000620">
        <property type="entry name" value="EamA_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00892">
        <property type="entry name" value="EamA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="25"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="51"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="83"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="140"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="164"/>
            <end position="186"/>
        </location>
    </feature>
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        <location>
            <begin position="198"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="230"/>
            <end position="250"/>
        </location>
    </feature>
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        <location>
            <begin position="262"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="285"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="domain" description="EamA" evidence="2">
        <location>
            <begin position="31"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="domain" description="EamA" evidence="2">
        <location>
            <begin position="169"/>
            <end position="302"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF11267.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="306" mass="32503" checksum="34FF4C1D15E827C6" modified="2006-05-30" version="1">MSERPTYEVPAATPGATPNTQWLRVAPALFLLLWSGGFVFLKLGLRHADPLTFLALRYACVVGLLIVPCLWIRPAFPPGIKPWLHLGMVGLLIQAGYFSFTYLSLKHGMSAGAVALVTSQQPILIGLLAPAIAGERVDRVRWLGLALGVTGAVIVITSRSSIDVATPAGLIFALLALLAMTGGTLWEKRFGTTVHPVASNLVQYAVGLVVTAPLAYALEPMHVAWTPELFGSLAYLVIGNSIIAISLLLAMIRHGEASRVSALFFLVPPMTAVVAFAVLGESIPLAAWPGMAMAAIGLYLVMGRRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="108" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LFP5</accession>
    <name>Q1LFP5_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Histidine kinase</fullName>
            <ecNumber evidence="2">2.7.13.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Rmet_4164</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="8">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="8">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.</text>
            <dbReference type="EC" id="2.7.13.3"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.7.13.3" evidence="2"/>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11031.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518653.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4164"/>
    <dbReference type="EnsemblBacteria" id="ABF11031">
        <property type="protein sequence ID" value="ABF11031"/>
        <property type="gene ID" value="Rmet_4164"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4164"/>
    <dbReference type="eggNOG" id="COG2205">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG4252">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016084_0_0_4"/>
    <dbReference type="OMA" id="YPLWSWL"/>
    <dbReference type="OrthoDB" id="1635706at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00082">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007890">
        <property type="entry name" value="CHASE2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003661">
        <property type="entry name" value="HisK_dim/P"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036097">
        <property type="entry name" value="HisK_dim/P_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000700">
        <property type="entry name" value="PAS-assoc_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004358">
        <property type="entry name" value="Sig_transdc_His_kin-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017181">
        <property type="entry name" value="Sig_transdc_His_kin_CHASE2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05226">
        <property type="entry name" value="CHASE2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037347">
        <property type="entry name" value="STHK_CHASE2_PAS_prd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00344">
        <property type="entry name" value="BCTRLSENSOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM01080">
        <property type="entry name" value="CHASE2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47384">
        <property type="entry name" value="SSF47384"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="SSF55874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50113">
        <property type="entry name" value="PAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="7" id="KW-0614">Plasmid</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="35"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="324"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="348"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="domain" description="PAC" evidence="6">
        <location>
            <begin position="548"/>
            <end position="598"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="5">
        <location>
            <begin position="602"/>
            <end position="816"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABF11031.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="819" mass="88113" checksum="686D38588009D381" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LFT8</accession>
    <name>Q1LFT8_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Heavy metal cation tricomponent efflux, membrane fusion protein HmyB (CzcB-like)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">hmyB</name>
        <name evidence="4" type="ordered locus">Rmet_4121</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="5">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="5">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10988.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518616.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4121"/>
    <dbReference type="EnsemblBacteria" id="ABF10988">
        <property type="protein sequence ID" value="ABF10988"/>
        <property type="gene ID" value="Rmet_4121"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4121"/>
    <dbReference type="eggNOG" id="COG0845">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018816_13_3_4"/>
    <dbReference type="OMA" id="GAFHLNN"/>
    <dbReference type="OrthoDB" id="1174564at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032317">
        <property type="entry name" value="HlyD_D23"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006143">
        <property type="entry name" value="RND_pump_MFP"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16576">
        <property type="entry name" value="HlyD_D23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01730">
        <property type="entry name" value="RND_mfp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0614">Plasmid</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193100" evidence="2">
        <location>
            <begin position="21"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="domain" description="HlyD_D23" evidence="3">
        <location>
            <begin position="60"/>
            <end position="302"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF16576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF10988.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="384" mass="41180" checksum="6E1CEB4EC0D6871A" modified="2006-05-30" version="1">MRPQFLLSFAAAAVMMLSLAGCSDDPAPAAEAPKLPPGIVKPEENLKRTLKVAPVAASPFSEMLRVAGRIDFDEQRVSRIGASVTGRVTDLYAVLGQEVKAGQVLARLHSSELGAAQMAFLKAEAQNTLQARNAERARQLFAADVIGRAELQRRESEYAIAAAEARAYRDQLRVLGMSAASIATLAKSGSIESYSPVFSSINGTVVERNVAQGQVVQPADALYTVADLSRVWVVAEVPEQQAAQVAEGQSVDIEVPSLANSNGRITGKLIYVGRTVNPQSRTVLVRTELQNKDGRLKPAMLATMLIAAKPVELLVVPGSAVVREGNDEQVYVEVGDGQYRLTKVKLGPESDGMRVVQSGIKPGDRVVVEGAFHLDNERKQQEQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="67" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LG11</accession>
    <name>Q1LG11_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Extra-cytoplasmic solute receptor protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">bug</name>
        <name evidence="4" type="ordered locus">Rmet_4047</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="5">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="5">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Periplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the UPF0065 (bug) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10915.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4047"/>
    <dbReference type="EnsemblBacteria" id="ABF10915">
        <property type="protein sequence ID" value="ABF10915"/>
        <property type="gene ID" value="Rmet_4047"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4047"/>
    <dbReference type="eggNOG" id="COG3181">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045683_0_0_4"/>
    <dbReference type="OMA" id="AYVTNTM"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042597">
        <property type="term" value="C:periplasmic space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005064">
        <property type="entry name" value="BUG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042100">
        <property type="entry name" value="Bug_dom1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42928">
        <property type="entry name" value="PTHR42928"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03401">
        <property type="entry name" value="TctC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017082">
        <property type="entry name" value="YflP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-0675">Receptor</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193504" evidence="3">
        <location>
            <begin position="30"/>
            <end position="329"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF10915.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="329" mass="34706" checksum="84D69DA738A9A0EA" modified="2006-05-30" version="1">MKSEMKSVLRSLLCGAAALTMSFANLASAADAWPTKPITLIVPWAAGGSTDILARTLSEQLTKSLGQPVIVDNRPGASGNIGSAMVARAKPDGYTLLIGSMSTHAMNPALMPNMPFKGVDDFTPLGLLAYVTNTMVVNASVPVHNVKELIAYAKANPGKVAYASAGPGSTNHLSAVLFEKMAGVQLLHVPYKGGAPAVVDTVAGQTQLLFSAGTQTLTHVKSGKLRLLAVTEAKRSPLLPNVPTVGETLPGYELSVWYGAFGPKNMPAELVTRLNNEINRAMALPDVKAKMESIGVETATSTPQEFGKILRRDADRYGKLIRELGIQGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="101" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LGA3</accession>
    <name>Q1LGA3_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Cytochrome-c oxidase</fullName>
            <ecNumber evidence="4">7.1.1.9</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ordered locus">Rmet_3955</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="20">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="20" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="20">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Periplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the cytochrome c oxidase subunit 2 family.</text>
    </comment>
    <dbReference type="EC" id="7.1.1.9" evidence="4"/>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10823.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518462.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3955"/>
    <dbReference type="EnsemblBacteria" id="ABF10823">
        <property type="protein sequence ID" value="ABF10823"/>
        <property type="gene ID" value="Rmet_3955"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3955"/>
    <dbReference type="eggNOG" id="COG1622">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG2010">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036876_1_0_4"/>
    <dbReference type="OMA" id="LEWWLIG"/>
    <dbReference type="OrthoDB" id="1654242at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042597">
        <property type="term" value="C:periplasmic space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004129">
        <property type="term" value="F:cytochrome-c oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.760.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.420">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045187">
        <property type="entry name" value="CcO_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002429">
        <property type="entry name" value="CcO_II-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001505">
        <property type="entry name" value="Copper_CuA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008972">
        <property type="entry name" value="Cupredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009056">
        <property type="entry name" value="Cyt_c-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036909">
        <property type="entry name" value="Cyt_c-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036257">
        <property type="entry name" value="Cyt_c_oxidase_su2_TM_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22888">
        <property type="entry name" value="PTHR22888"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00116">
        <property type="entry name" value="COX2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00034">
        <property type="entry name" value="Cytochrom_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46626">
        <property type="entry name" value="SSF46626"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49503">
        <property type="entry name" value="SSF49503"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81464">
        <property type="entry name" value="SSF81464"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00078">
        <property type="entry name" value="COX2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50857">
        <property type="entry name" value="COX2_CUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51007">
        <property type="entry name" value="CYTC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-0186">Copper</keyword>
    <keyword evidence="10" id="KW-0249">Electron transport</keyword>
    <keyword evidence="6 15" id="KW-0349">Heme</keyword>
    <keyword evidence="12 15" id="KW-0408">Iron</keyword>
    <keyword evidence="14 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="19" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="19" id="KW-0614">Plasmid</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-1278">Translocase</keyword>
    <keyword evidence="7 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 16" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="41"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="83"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="domain" description="COX2_CUA" evidence="17">
        <location>
            <begin position="112"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="domain" description="Cytochrome c" evidence="18">
        <location>
            <begin position="287"/>
            <end position="380"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007866"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="ABF10823.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="391" mass="42049" checksum="9C7C117F82D9E328" modified="2006-05-30" version="1">MAIAIALVIIVVASVLFHFLSPWWATPLASNWKQMDDTLTITLVITGLFFVVINLLVGYIVWRFRHRAGHRAAYAPENPKLERWLIGLTAVCIIALLAPGLIVYAEYVRPPRDAMVMEVVGQQWQWAFRFPGKSGQLGTTDPRFVSGTNPLGLNPDDPNGQDNILVMGPEVHLPLNRPIKVLLRSKDVLHDFYVPPFRARMNMVPGMVTSFWFTPTKTGRFDILCAQLCGLGHYNMRGHVVVEEQAAFDTWLAKQQTFAMTLAKVAAPTAAPAEGPAVAGASAGAADLVAQGRALAQSKGCVGCHSVDGSVGVGPSWKGLYGSTQTFSNGSSATVNDAFLKTEIEDPQARIVKGFGPVMPKLPVTDADVVALSAYIRSLGAGATGTPQAGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LGM2</accession>
    <name>Q1LGM2_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">L-carnitine dehydratase/bile acid-inducible protein F (CoA-transferase family)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_3834</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10704.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518350.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3834"/>
    <dbReference type="EnsemblBacteria" id="ABF10704">
        <property type="protein sequence ID" value="ABF10704"/>
        <property type="gene ID" value="Rmet_3834"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3834"/>
    <dbReference type="eggNOG" id="COG1804">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033975_1_1_4"/>
    <dbReference type="OMA" id="LIVCNIS"/>
    <dbReference type="OrthoDB" id="969868at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1540.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10540">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003673">
        <property type="entry name" value="CoA-Trfase_fam_III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044855">
        <property type="entry name" value="CoA-Trfase_III_dom3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023606">
        <property type="entry name" value="CoA-Trfase_III_dom_1_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02515">
        <property type="entry name" value="CoA_transf_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89796">
        <property type="entry name" value="SSF89796"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF10704.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="390" mass="41894" checksum="A5579F5B343ADFD8" modified="2006-05-30" version="1">MSRPLEGITVVSLEQAIAAPFCTRQLADLGARVIKIERPGPGDFARAYDGRVRGLSSHFVWTNRSKESLTLDVKAPAAAPVLDSLLAKTDVLIQNLAPGASTRLGLDYETLSKRFPRLIVCDISGYGGDGPYRDKKAYDLLVQSESGFVSVTGTPEEGVKAGASVADIAAGMYAYSNVLAALIERGQTGRGKHIDVSMLESMVEWMNFPMYYAFDGAEPPPRAGASHATIYPYGPFPTGDGKTVMLGLQNEREWAAFCKVVLHEPDLATHAAFATNSLRSANRAQLRARIVEAFGRLTAAQVVERLEEAKIANANVNTMADVWAHPQLAARKRWRTVDTPAGQIPALLPPGMTDARMDPVPAVGEHTDAILAEAGCTAEQIARLHADGVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LGR1</accession>
    <name>Q1LGR1_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Lipase helper protein</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Lipase modulator</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">lipB</name>
        <name evidence="11" type="ordered locus">Rmet_3795</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="12">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="12">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell inner membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
            <orientation evidence="1">Periplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the lipase chaperone family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10665.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518313.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3795"/>
    <dbReference type="EnsemblBacteria" id="ABF10665">
        <property type="protein sequence ID" value="ABF10665"/>
        <property type="gene ID" value="Rmet_3795"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3795"/>
    <dbReference type="eggNOG" id="COG5380">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_064928_1_0_4"/>
    <dbReference type="OMA" id="QQYIDYK"/>
    <dbReference type="OrthoDB" id="1337848at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004961">
        <property type="entry name" value="Lipase_chaperone"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03280">
        <property type="entry name" value="Lipase_chap"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-0614">Plasmid</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="31"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="326"/>
            <end position="351"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010358"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00030948"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00031542"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ABF10665.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="351" mass="38577" checksum="E16CDF58D1BE0156" modified="2006-05-30" version="1">MISSERRPGVTLVLVVAGLVGAAVYWATAPTEPAQGPLPARSRTDARPELSASPTDSMRARNITEMPSLAGVDIPPGPEADAQGNLRLTRALRTYFDYFLSASHDAGDAAALDQLVRDDIRKHVPQPAEDQAWRLWQRYRAYLSEMQPKATDGPMTSVIGAPDTSQVQRLRALIADRNAARARHLPDVAAIWFDDEQTYDDAMLARLEIATQPGLDDAERQRRLSTLDATLPESVRAAREAEARPQAISKTIASMQTAGRSPQDIGASLAQAYGPEVAQRYQQQAQAEQAWKQRYDDYAAQRSQIVSFAGLSEQDRAQQLETLRRQTFTNPSEALQAEVMDKAVGGRRAAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-10-07" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LGY3</accession>
    <name>Q1LGY3_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Extra-cytoplasmic Solute Receptor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">bug</name>
        <name evidence="3" type="ordered locus">Rmet_3721</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Periplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the UPF0065 (bug) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10593.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3721"/>
    <dbReference type="EnsemblBacteria" id="ABF10593">
        <property type="protein sequence ID" value="ABF10593"/>
        <property type="gene ID" value="Rmet_3721"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3721"/>
    <dbReference type="eggNOG" id="COG3181">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045683_0_0_4"/>
    <dbReference type="OMA" id="FVMSSWT"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042597">
        <property type="term" value="C:periplasmic space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005064">
        <property type="entry name" value="BUG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042100">
        <property type="entry name" value="Bug_dom1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42928">
        <property type="entry name" value="PTHR42928"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03401">
        <property type="entry name" value="TctC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017082">
        <property type="entry name" value="YflP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-0675">Receptor</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF10593.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="345" mass="36770" checksum="82404F2FA82564DE" modified="2006-05-30" version="1">MFRHADAIKRIKVGDDVLRIKHQASRIARVVAIAVAGLSIHHGAIAADAWPAKPIKVIVPYTPGGSTDTVARVVFEKVSQSLGQPIVIENKPGANSTLGVGVVARSAPDGYTFVSVLAAYSANMSLYSKLSYKPTDLAPVAEMAELPLFLFSSKKLPVKTVAELVDYGKKHPDTLTFGSSGIGSSAHLTGERLAMESKVKMTHIPYNGSAPILPALVSGEISMAFDPLLVPMPHVKSGKINVLAVASAKRWPSEPNIPTMEESGFPGFVMSSWTGLLAPAGTPQPIIDRMAKEIAAATRNPDVTKKLTELGFIPVGGTPEEFRKLIERDTSRYGQIVKAGKITLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LGZ2</accession>
    <name>Q1LGZ2_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Salicylate 5-hydroxylase (Oxygenase small subunit)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">nagH</name>
        <name evidence="2" type="ordered locus">Rmet_3712</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10584.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518232.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3712"/>
    <dbReference type="EnsemblBacteria" id="ABF10584">
        <property type="protein sequence ID" value="ABF10584"/>
        <property type="gene ID" value="Rmet_3712"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3712"/>
    <dbReference type="eggNOG" id="COG5517">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_137193_0_0_4"/>
    <dbReference type="OMA" id="YYQRHVI"/>
    <dbReference type="OrthoDB" id="1736098at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032710">
        <property type="entry name" value="NTF2-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037401">
        <property type="entry name" value="SnoaL-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13577">
        <property type="entry name" value="SnoaL_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54427">
        <property type="entry name" value="SSF54427"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SnoaL-like" evidence="1">
        <location>
            <begin position="8"/>
            <end position="138"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13577"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF10584.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="158" mass="17688" checksum="845F869C1B1F7739" modified="2006-05-30" version="1">MTTVETWQQVLLLYADYTAALDNKQWEKWPEFFLEQCVCNAQSHEAHICGLPMATPAFESCGLLMEKACANGIGAQDLHYRQQVGIPRILEDSGSQIRTETSYAVFRTTPDDTSAIHSVGRYLDTIARTDAGLKFASRVCILERRAIPDLLAGRCMSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LGZ6</accession>
    <name>Q1LGZ6_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Major facilitator superfamily MFS_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">pcaK2</name>
        <name evidence="7" type="ordered locus">Rmet_3708</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="8">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="8">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10580.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518228.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3708"/>
    <dbReference type="EnsemblBacteria" id="ABF10580">
        <property type="protein sequence ID" value="ABF10580"/>
        <property type="gene ID" value="Rmet_3708"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3708"/>
    <dbReference type="eggNOG" id="COG2814">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001265_46_4_4"/>
    <dbReference type="OMA" id="WYGLGTW"/>
    <dbReference type="OrthoDB" id="1121832at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="SSF103473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00216">
        <property type="entry name" value="SUGAR_TRANSPORT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00217">
        <property type="entry name" value="SUGAR_TRANSPORT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0614">Plasmid</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="21"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="58"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="90"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="114"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="148"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="176"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="259"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="298"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="323"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="348"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="382"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="411"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="domain" description="MFS" evidence="6">
        <location>
            <begin position="24"/>
            <end position="437"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABF10580.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="441" mass="46531" checksum="951BC259543F5DE7" modified="2006-05-30" version="1">MTTQRLDIRAHINERKMSGYQWLLLVLCFLIVATDGMDVAIMGFLAPDITREWGISKAAFGVVMSAAPIGLAIGALVVGPLSDRFGRKKLLVGSIALFGVFNLLSAFADGTATLSLLRFLTGLGLGAAMPSTTTLLSEYVPERSRSMLLATMFTGFNLGSALVGFGAAALLPHHGWRAVLVAGGAIPLICLPFYIMLIPESARFMVVRNVAPARIARTLRRVIGSVVADDTVFTISEPPVSGKQPVRTLLSSGYRSMTLALWVTYFMGLLVIYLLSGWLPTLIKDAGLPIDRAANLTALFQLGGTVGALVVGYCMDRFAPHRVIGVSYVLGAMFILMLASGSVTSSMFALYVLLAGFCMSGAQTGLNAFAPQCYPTPVRATGVSWMLGIGRFGSITGSMAGGVLLSMGWSFSAVIAILAVPATMAAISIVSTKAQPRAVTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LH48</accession>
    <name>Q1LH48_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Type II/IV secretion system protein, ATPase domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">tadA</name>
        <name evidence="3" type="ordered locus">Rmet_3656</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the GSP E family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10528.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518183.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3656"/>
    <dbReference type="EnsemblBacteria" id="ABF10528">
        <property type="protein sequence ID" value="ABF10528"/>
        <property type="gene ID" value="Rmet_3656"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3656"/>
    <dbReference type="eggNOG" id="COG4962">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005379_4_1_4"/>
    <dbReference type="OMA" id="GLPGMCT"/>
    <dbReference type="OrthoDB" id="473670at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001482">
        <property type="entry name" value="T2SS/T4SS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00437">
        <property type="entry name" value="T2SSE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="T2SP_E" evidence="2">
        <location>
            <begin position="96"/>
            <end position="366"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006611"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF10528.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="438" mass="48516" checksum="1AFA5C3474DF1F00" modified="2006-05-30" version="1">MTDAIEFSDNAAAPFPVSQEFHNIKEAAHEHLLTRIEELGAEFGRWSRAAIQRFVDLELESFTRLRRIPINEAELRQISEALTNELAGFGPIEDLLNDPAVEDILVNGHMDVYVSRHGVLERIPVRFADGGHLLRIVRRILAPIGRRLDESNPMVDARLPDGGRINVIIPPLALEGPVVSIRKFRKDPLTPLDLQTLGTMSPEITELLDAAVRARCNILVSGGTSSGKTSLLNALATFIPSTERVITIEDTAELALNHPHVVRLESRPGGFEGTGTVTIRDLLRNSLRMRPDRIIVGEVRGGEVLEMLQAMSTGHDGSMGTIHASSPRECLYRLEMLAGFAGFQGSEVSLRRQIANAIDFIVQIGRLSNGRRRILSITEVTGLGDNIISTQELYRHEPVQNPDGTESDRWQSLGILPHSPKLARMRPANFSLDDRFDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LH70</accession>
    <name>Q1LH70_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Ornithine cyclodeaminase/mu-crystallin</fullName>
            <ecNumber evidence="1">4.3.1.12</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_3634</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EC" id="4.3.1.12" evidence="1"/>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10506.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3634"/>
    <dbReference type="EnsemblBacteria" id="ABF10506">
        <property type="protein sequence ID" value="ABF10506"/>
        <property type="gene ID" value="Rmet_3634"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3634"/>
    <dbReference type="eggNOG" id="COG2423">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_042088_2_1_4"/>
    <dbReference type="OMA" id="THEALHE"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008473">
        <property type="term" value="F:ornithine cyclodeaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1780.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003462">
        <property type="entry name" value="ODC_Mu_crystall"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023401">
        <property type="entry name" value="ODC_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13812">
        <property type="entry name" value="PTHR13812"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02423">
        <property type="entry name" value="OCD_Mu_crystall"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001439">
        <property type="entry name" value="CryM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0456">Lyase</keyword>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF10506.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="325" mass="34102" checksum="1C1FDD2B5C626905" modified="2006-05-30" version="1">MKHCVDCIPKYKQDAPMLHITDTLIDRHVTAADAQAAMLDAFRSFGRGQAAMQERIRTEAGGVKLSTLGAVIPDQQVAGAKVYTTIAGQFSFVILLFSTVDGRPLASFDAGAITRLRTAACTVLAAKRLARSDAQTLALFGAGTQGVQHALQLSAALPFQRILICDPYADAEAATRLSAQCGVSVSFAQAEEAVAAADIIVTASRSTTPLFPGAIVKPGTFIAAIGSSLPHTRELDDVALSRAAAVVVEWRPQSLREAGDLVLADKAVLPDEKIVELADVLLDTAPTRKHADDILIYKSVGVGLEDVALAGIAYQRITAAQAAAA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-02-05" modified="2022-05-25" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LH91</accession>
    <name>YIDC_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Membrane protein insertase YidC</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Foldase YidC</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Membrane integrase YidC</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Membrane protein YidC</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">yidC</name>
        <name type="ordered locus">Rmet_3613</name>
    </gene>
    <organism>
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Required for the insertion and/or proper folding and/or complex formation of integral membrane proteins into the membrane. Involved in integration of membrane proteins that insert both dependently and independently of the Sec translocase complex, as well as at least some lipoproteins. Aids folding of multispanning membrane proteins.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Interacts with the Sec translocase complex via SecD. Specifically interacts with transmembrane segments of nascent integral membrane proteins during membrane integration.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the OXA1/ALB3/YidC family. Type 1 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10485.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518027.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q1LH91"/>
    <dbReference type="SMR" id="Q1LH91"/>
    <dbReference type="STRING" id="266264.Rmet_3613"/>
    <dbReference type="EnsemblBacteria" id="ABF10485">
        <property type="protein sequence ID" value="ABF10485"/>
        <property type="gene ID" value="Rmet_3613"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3613"/>
    <dbReference type="eggNOG" id="COG0706">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016535_3_0_4"/>
    <dbReference type="OMA" id="INPVAGC"/>
    <dbReference type="OrthoDB" id="524437at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032977">
        <property type="term" value="F:membrane insertase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.98.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01810">
        <property type="entry name" value="YidC_type1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019998">
        <property type="entry name" value="Membr_insert_YidC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028055">
        <property type="entry name" value="Membr_insert_YidC/Oxa1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028053">
        <property type="entry name" value="Membr_insert_YidC_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001708">
        <property type="entry name" value="YidC/ALB3/OXA1/COX18"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038210">
        <property type="entry name" value="YidC_periplasmic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038221">
        <property type="entry name" value="YidC_periplasmic_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12428">
        <property type="entry name" value="PTHR12428"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02096">
        <property type="entry name" value="60KD_IMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14849">
        <property type="entry name" value="YidC_periplas"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00701">
        <property type="entry name" value="60KDINNERMP"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01900">
        <property type="entry name" value="YIDCPROTEIN"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03593">
        <property type="entry name" value="yidC_nterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03592">
        <property type="entry name" value="yidC_oxa1_cterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0997">Cell inner membrane</keyword>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0143">Chaperone</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-0653">Protein transport</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <keyword id="KW-0813">Transport</keyword>
    <feature type="chain" id="PRO_1000070150" description="Membrane protein insertase YidC">
        <location>
            <begin position="1"/>
            <end position="555"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="334"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="360"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="430"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="468"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="503"/>
            <end position="523"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="40"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01810"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="555" mass="61103" checksum="9D352F5ED57B9141" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="105" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LHM6</accession>
    <name>Q1LHM6_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">ATP-dependent DNA helicase Rep</fullName>
            <ecNumber evidence="6">3.6.4.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6 10" type="primary">rep</name>
        <name evidence="10" type="ordered locus">Rmet_3478</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Rep helicase is a single-stranded DNA-dependent ATPase involved in DNA replication; it can initiate unwinding at a nick in the DNA. It binds to the single-stranded DNA and acts in a progressive fashion along the DNA in the 3' to 5' direction.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + H2O = ADP + H(+) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.12"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="6">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the helicase family. UvrD subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.12" evidence="6"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10350.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517908.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3478"/>
    <dbReference type="EnsemblBacteria" id="ABF10350">
        <property type="protein sequence ID" value="ABF10350"/>
        <property type="gene ID" value="Rmet_3478"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3478"/>
    <dbReference type="eggNOG" id="COG0210">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004585_5_4_4"/>
    <dbReference type="OMA" id="HCANILI"/>
    <dbReference type="OrthoDB" id="137860at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008408">
        <property type="term" value="F:3'-5' exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003678">
        <property type="term" value="F:DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006268">
        <property type="term" value="P:DNA unwinding involved in DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.160">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01920">
        <property type="entry name" value="Helicase_Rep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013986">
        <property type="entry name" value="DExx_box_DNA_helicase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014017">
        <property type="entry name" value="DNA_helicase_UvrD-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000212">
        <property type="entry name" value="DNA_helicase_UvrD/REP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005752">
        <property type="entry name" value="Helicase_Rep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014016">
        <property type="entry name" value="UvrD-like_ATP-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11070">
        <property type="entry name" value="PTHR11070"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00580">
        <property type="entry name" value="UvrD-helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13361">
        <property type="entry name" value="UvrD_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51198">
        <property type="entry name" value="UVRD_HELICASE_ATP_BIND"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51217">
        <property type="entry name" value="UVRD_HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 6" id="KW-0235">DNA replication</keyword>
    <keyword evidence="6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6 7 10" id="KW-0347">Helicase</keyword>
    <keyword evidence="6 7 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 6 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UvrD-like helicase ATP-binding" evidence="8">
        <location>
            <begin position="3"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="domain" description="UvrD-like helicase C-terminal" evidence="9">
        <location>
            <begin position="292"/>
            <end position="587"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="24"/>
            <end position="31"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="289"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001665"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01920"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ABF10350.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="695" mass="78374" checksum="33E81B3384A77DE8" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="100" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LHN7</accession>
    <name>Q1LHN7_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Bifunctional cytochrome P450/NADPH--P450 reductase</fullName>
        </recommendedName>
        <domain>
            <recommendedName>
                <fullName evidence="9">Cytochrome P450</fullName>
                <ecNumber evidence="9">1.14.14.1</ecNumber>
            </recommendedName>
        </domain>
        <domain>
            <recommendedName>
                <fullName evidence="9">NADPH--cytochrome P450 reductase</fullName>
                <ecNumber evidence="9">1.6.2.4</ecNumber>
            </recommendedName>
        </domain>
    </protein>
    <gene>
        <name evidence="13" type="ordered locus">Rmet_3467</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>NADPH + 2 oxidized [cytochrome P450] = H(+) + NADP(+) + 2 reduced [cytochrome P450]</text>
            <dbReference type="Rhea" id="RHEA:24040"/>
            <dbReference type="Rhea" id="RHEA-COMP:14627"/>
            <dbReference type="Rhea" id="RHEA-COMP:14628"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:55376"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
            <dbReference type="EC" id="1.6.2.4"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>an organic molecule + O2 + reduced [NADPH--hemoprotein reductase] = an alcohol + H(+) + H2O + oxidized [NADPH--hemoprotein reductase]</text>
            <dbReference type="Rhea" id="RHEA:17149"/>
            <dbReference type="Rhea" id="RHEA-COMP:11964"/>
            <dbReference type="Rhea" id="RHEA-COMP:11965"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:30879"/>
            <dbReference type="ChEBI" id="CHEBI:57618"/>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
            <dbReference type="ChEBI" id="CHEBI:142491"/>
            <dbReference type="EC" id="1.14.14.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
        <cofactor evidence="9">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="9 10">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1 9">In the N-terminal section; belongs to the cytochrome P450 family.</text>
    </comment>
    <dbReference type="EC" id="1.14.14.1" evidence="9"/>
    <dbReference type="EC" id="1.6.2.4" evidence="9"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10339.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517897.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3467"/>
    <dbReference type="EnsemblBacteria" id="ABF10339">
        <property type="protein sequence ID" value="ABF10339"/>
        <property type="gene ID" value="Rmet_3467"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3467"/>
    <dbReference type="eggNOG" id="COG0369">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG2124">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001570_7_0_4"/>
    <dbReference type="OMA" id="WKKAHNI"/>
    <dbReference type="OrthoDB" id="520970at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070330">
        <property type="term" value="F:aromatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003958">
        <property type="term" value="F:NADPH-hemoprotein reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019344">
        <property type="term" value="P:cysteine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.990.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023206">
        <property type="entry name" value="Bifunctional_P450_P450_red"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003097">
        <property type="entry name" value="CysJ-like_FAD-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017927">
        <property type="entry name" value="FAD-bd_FR_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008254">
        <property type="entry name" value="Flavodoxin/NO_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001709">
        <property type="entry name" value="Flavoprot_Pyr_Nucl_cyt_Rdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029039">
        <property type="entry name" value="Flavoprotein-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039261">
        <property type="entry name" value="FNR_nucleotide-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023173">
        <property type="entry name" value="NADPH_Cyt_P450_Rdtase_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001433">
        <property type="entry name" value="OxRdtase_FAD/NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017938">
        <property type="entry name" value="Riboflavin_synthase-like_b-brl"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00667">
        <property type="entry name" value="FAD_binding_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00258">
        <property type="entry name" value="Flavodoxin_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00175">
        <property type="entry name" value="NAD_binding_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000209">
        <property type="entry name" value="Bifunctional_P450_P450R"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00371">
        <property type="entry name" value="FPNCR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="SSF48264"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52218">
        <property type="entry name" value="SSF52218"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52343">
        <property type="entry name" value="SSF52343"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63380">
        <property type="entry name" value="SSF63380"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51384">
        <property type="entry name" value="FAD_FR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50902">
        <property type="entry name" value="FLAVODOXIN_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="8" id="KW-0198">Cysteine biosynthesis</keyword>
    <keyword evidence="9" id="KW-0249">Electron transport</keyword>
    <keyword evidence="4 9" id="KW-0274">FAD</keyword>
    <keyword evidence="2 9" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="3 9" id="KW-0288">FMN</keyword>
    <keyword evidence="9 10" id="KW-0349">Heme</keyword>
    <keyword evidence="9 10" id="KW-0408">Iron</keyword>
    <keyword evidence="9 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 9" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="5 9" id="KW-0521">NADP</keyword>
    <keyword evidence="6 9 13" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Flavodoxin-like" evidence="11">
        <location>
            <begin position="491"/>
            <end position="631"/>
        </location>
    </feature>
    <feature type="domain" description="FAD-binding FR-type" evidence="12">
        <location>
            <begin position="663"/>
            <end position="909"/>
        </location>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="10">
        <location>
            <position position="410"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR000209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR000209-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ABF10339.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="1064" mass="116877" checksum="73EE0EAAEAE680E1" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="50" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LHV2</accession>
    <name>Q1LHV2_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_3402</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10274.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3402"/>
    <dbReference type="EnsemblBacteria" id="ABF10274">
        <property type="protein sequence ID" value="ABF10274"/>
        <property type="gene ID" value="Rmet_3402"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3402"/>
    <dbReference type="eggNOG" id="ENOG50316GH">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1414142_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="168"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="26"/>
            <end position="59"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF10274.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="192" mass="21220" checksum="71A8FB430BF87D6D" modified="2006-05-30" version="1">MCDASSLAKKAGADAGQLRTPRAKNFNKTCADHPRTARERWITHTAHRGSGRTVDRRKPNRPMMGNTMPLRPRQRAGHRLENPVAKHFDPLHALPHALWWQPGVYGSVPAVLLWIVARLPAEALASVFSTPYASALFNHAGDTVFVTGWLASAVWLWFTRKKPNLPDRNVAPAGDERPAAGQHAPHHGHRPG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-10-31" modified="2022-08-03" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LI46</accession>
    <name>RS17_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">30S ribosomal protein S17</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpsQ</name>
        <name type="ordered locus">Rmet_3308</name>
    </gene>
    <organism>
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">One of the primary rRNA binding proteins, it binds specifically to the 5'-end of 16S ribosomal RNA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the 30S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uS17 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10180.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008642939.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q1LI46"/>
    <dbReference type="SMR" id="Q1LI46"/>
    <dbReference type="STRING" id="266264.Rmet_3308"/>
    <dbReference type="PRIDE" id="Q1LI46"/>
    <dbReference type="EnsemblBacteria" id="ABF10180">
        <property type="protein sequence ID" value="ABF10180"/>
        <property type="gene ID" value="Rmet_3308"/>
    </dbReference>
    <dbReference type="GeneID" id="60826609"/>
    <dbReference type="KEGG" id="rme:Rmet_3308"/>
    <dbReference type="eggNOG" id="COG0186">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_073626_1_1_4"/>
    <dbReference type="OMA" id="HPMYGKF"/>
    <dbReference type="OrthoDB" id="2035880at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01345_B">
        <property type="entry name" value="Ribosomal_S17_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019984">
        <property type="entry name" value="Ribosomal_S17"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000266">
        <property type="entry name" value="Ribosomal_S17/S11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019979">
        <property type="entry name" value="Ribosomal_S17_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10744">
        <property type="entry name" value="PTHR10744"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00366">
        <property type="entry name" value="Ribosomal_S17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00973">
        <property type="entry name" value="RIBOSOMALS17"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03635">
        <property type="entry name" value="uS17_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00056">
        <property type="entry name" value="RIBOSOMAL_S17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <feature type="chain" id="PRO_0000255690" description="30S ribosomal protein S17">
        <location>
            <begin position="1"/>
            <end position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01345"/>
        </source>
    </evidence>
    <sequence length="92" mass="10640" checksum="8ED46A43EFEC643A" modified="2006-05-30" version="1">MTEAAKTETSLRRTLVGRVVSDKMDKTVTVLIENRVKHPLYGKYVLRSKKYHAHDEANQYKEGDKVEIQETRPLSRTKSWVVSRLVEAARVI</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-02-05" modified="2022-08-03" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LIB3</accession>
    <name>HIS3_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Phosphoribosyl-AMP cyclohydrolase</fullName>
            <shortName evidence="1">PRA-CH</shortName>
            <ecNumber evidence="1">3.5.4.19</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">hisI</name>
        <name type="ordered locus">Rmet_3241</name>
    </gene>
    <organism>
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the hydrolysis of the adenine ring of phosphoribosyl-AMP.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>1-(5-phospho-beta-D-ribosyl)-5'-AMP + H2O = 1-(5-phospho-beta-D-ribosyl)-5-[(5-phospho-beta-D-ribosylamino)methylideneamino]imidazole-4-carboxamide</text>
            <dbReference type="Rhea" id="RHEA:20049"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:58435"/>
            <dbReference type="ChEBI" id="CHEBI:59457"/>
            <dbReference type="EC" id="3.5.4.19"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="1">Binds 1 Mg(2+) ion per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="1">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-histidine biosynthesis; L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 3/9.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the PRA-CH family.</text>
    </comment>
    <dbReference type="EC" id="3.5.4.19" evidence="1"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10113.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008643058.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q1LIB3"/>
    <dbReference type="SMR" id="Q1LIB3"/>
    <dbReference type="STRING" id="266264.Rmet_3241"/>
    <dbReference type="EnsemblBacteria" id="ABF10113">
        <property type="protein sequence ID" value="ABF10113"/>
        <property type="gene ID" value="Rmet_3241"/>
    </dbReference>
    <dbReference type="GeneID" id="60825565"/>
    <dbReference type="KEGG" id="rme:Rmet_3241"/>
    <dbReference type="eggNOG" id="COG0139">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_048577_5_0_4"/>
    <dbReference type="OMA" id="TGYRSCF"/>
    <dbReference type="OrthoDB" id="1842189at2"/>
    <dbReference type="UniPathway" id="UPA00031">
        <property type="reaction ID" value="UER00008"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004635">
        <property type="term" value="F:phosphoribosyl-AMP cyclohydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000105">
        <property type="term" value="P:histidine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.810">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01021">
        <property type="entry name" value="HisI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026660">
        <property type="entry name" value="PRA-CH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002496">
        <property type="entry name" value="PRib_AMP_CycHydrolase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038019">
        <property type="entry name" value="PRib_AMP_CycHydrolase_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01502">
        <property type="entry name" value="PRA-CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF141734">
        <property type="entry name" value="SSF141734"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0368">Histidine biosynthesis</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <feature type="chain" id="PRO_1000063430" description="Phosphoribosyl-AMP cyclohydrolase">
        <location>
            <begin position="1"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="78"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="79"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="80"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="82"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="96"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="103"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01021"/>
        </source>
    </evidence>
    <sequence length="135" mass="15484" checksum="6B6B17555E0321B3" modified="2006-05-30" version="1">MAKKWLNKVKWDDNGLVPVIVQEVGSNDVLMFAFMNRDALQRTVELGEAVFWSRSRKRLWHKGEESGHVQKVHEIRLDCDEDVVLLKVTQIDSIACHTGRHSCFFQKFEGDVESGDWQTVEPVLKDPSSIYAAKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="49" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LIE2</accession>
    <name>Q1LIE2_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_3212</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10084.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3212"/>
    <dbReference type="EnsemblBacteria" id="ABF10084">
        <property type="protein sequence ID" value="ABF10084"/>
        <property type="gene ID" value="Rmet_3212"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3212"/>
    <dbReference type="HOGENOM" id="CLU_1523939_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="77"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="147"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="150"/>
            <end position="176"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF10084.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="176" mass="18909" checksum="6B8D9FCE96809DEF" modified="2006-05-30" version="1">MCFTALKHTSARPISTAHRIRRGIGLGGSWRDGVRCMTGMRIRELVCNPRVCQMVVSAPRSTARGVSRRGWLPGAPACVLLLVLVLVLVLVEALLVDLLVERVPTGRCVAIPCSPIASIADVSVCRRYTRVAAGLQPPVHARHMLRQSGRVRHSGSTVPPLRLSSGSGGNHASRGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LIH9</accession>
    <name>Q1LIH9_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1 2">Protein ApaG</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2 4" type="primary">apaG</name>
        <name evidence="4" type="ordered locus">Rmet_3175</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10047.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008647582.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3175"/>
    <dbReference type="EnsemblBacteria" id="ABF10047">
        <property type="protein sequence ID" value="ABF10047"/>
        <property type="gene ID" value="Rmet_3175"/>
    </dbReference>
    <dbReference type="GeneID" id="60825468"/>
    <dbReference type="KEGG" id="rme:Rmet_3175"/>
    <dbReference type="eggNOG" id="COG2967">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_128074_0_0_4"/>
    <dbReference type="OMA" id="YVSGCNL"/>
    <dbReference type="OrthoDB" id="1835593at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1470">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00791">
        <property type="entry name" value="ApaG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007474">
        <property type="entry name" value="ApaG_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036767">
        <property type="entry name" value="ApaG_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023065">
        <property type="entry name" value="Uncharacterised_ApaG"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04379">
        <property type="entry name" value="DUF525"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110069">
        <property type="entry name" value="SSF110069"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51087">
        <property type="entry name" value="APAG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ApaG" evidence="3">
        <location>
            <begin position="1"/>
            <end position="124"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00017693"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00791"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF10047.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="124" mass="13877" checksum="694072CA8C8412C9" modified="2006-05-30" version="1">MSEYAFSVAVRTQYMPDQSDPDRGRYAFAYTITIHNTGEVAAQLISRHWIITDSDNSTQEVSGLGVVGHQPLLKPGEHFEYSSWATISTPVGSMKGDYFCVAEDGHRFEVPIPEFALVLPRMLH</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-06-10" modified="2022-08-03" version="102" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LIM8</accession>
    <name>DDL_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">D-alanine--D-alanine ligase</fullName>
            <ecNumber evidence="2">6.3.2.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">D-Ala-D-Ala ligase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="2">D-alanylalanine synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="2" type="primary">ddl</name>
        <name type="ordered locus">Rmet_3126</name>
    </gene>
    <organism>
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Cell wall formation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>ATP + 2 D-alanine = ADP + D-alanyl-D-alanine + H(+) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:11224"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57416"/>
            <dbReference type="ChEBI" id="CHEBI:57822"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.2.4"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="1">Binds 2 magnesium or manganese ions per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Cell wall biogenesis; peptidoglycan biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the D-alanine--D-alanine ligase family.</text>
    </comment>
    <dbReference type="EC" id="6.3.2.4" evidence="2"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09998.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517618.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q1LIM8"/>
    <dbReference type="SMR" id="Q1LIM8"/>
    <dbReference type="STRING" id="266264.Rmet_3126"/>
    <dbReference type="EnsemblBacteria" id="ABF09998">
        <property type="protein sequence ID" value="ABF09998"/>
        <property type="gene ID" value="Rmet_3126"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3126"/>
    <dbReference type="eggNOG" id="COG1181">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039268_1_2_4"/>
    <dbReference type="OMA" id="NTTPGMT"/>
    <dbReference type="OrthoDB" id="764798at2"/>
    <dbReference type="UniPathway" id="UPA00219"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008716">
        <property type="term" value="F:D-alanine-D-alanine ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009252">
        <property type="term" value="P:peptidoglycan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008360">
        <property type="term" value="P:regulation of cell shape"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00047">
        <property type="entry name" value="Dala_Dala_lig"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011761">
        <property type="entry name" value="ATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013815">
        <property type="entry name" value="ATP_grasp_subdomain_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000291">
        <property type="entry name" value="D-Ala_lig_Van_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005905">
        <property type="entry name" value="D_ala_D_ala"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011095">
        <property type="entry name" value="Dala_Dala_lig_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011127">
        <property type="entry name" value="Dala_Dala_lig_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016185">
        <property type="entry name" value="PreATP-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07478">
        <property type="entry name" value="Dala_Dala_lig_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01820">
        <property type="entry name" value="Dala_Dala_lig_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF039102">
        <property type="entry name" value="Ddl/VanB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52440">
        <property type="entry name" value="SSF52440"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01205">
        <property type="entry name" value="D_ala_D_alaTIGR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50975">
        <property type="entry name" value="ATP_GRASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00843">
        <property type="entry name" value="DALA_DALA_LIGASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00844">
        <property type="entry name" value="DALA_DALA_LIGASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0133">Cell shape</keyword>
    <keyword id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0464">Manganese</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0573">Peptidoglycan synthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000341160" description="D-alanine--D-alanine ligase">
        <location>
            <begin position="1"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="domain" description="ATP-grasp" evidence="2">
        <location>
            <begin position="113"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="139"/>
            <end position="194"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="266"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="279"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="279"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="281"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00047"/>
        </source>
    </evidence>
    <sequence length="327" mass="34968" checksum="E9968DC61E896386" modified="2006-05-30" version="1">MSFVAHPNIDPKSLGKVGVLLGGKSAEREISLLSGNGVLAALKSRGVDAHPFDPGLQPISELAAAGFDRVFIALHGRYGEDGTMQGLLEQLGIPYTGSGVLASALAMDKQATKRLWMTHGLATPRFAMLYANTDFDAVVADLGLPLIVKPAREGSSIGLTKVIAADQMRAAFEKAAGLDADVIAETFIDGAELTCPIVGEGPTAEALPVIKIVAPESNYDYQNKYFTDDTQYLCPSTLPDALEREVRALAVEAFRVLGCRGWARADVMLTRDGKPYLLEMNTSPGMTGHSLVPMAARANGISYEDFVMQVLAAATLDLHPNEHWKPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="102" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LIR0</accession>
    <name>Q1LIR0_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Signal recognition particle protein</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Fifty-four homolog</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7 9" type="primary">ffh</name>
        <name evidence="9" type="ordered locus">Rmet_3094</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Involved in targeting and insertion of nascent membrane proteins into the cytoplasmic membrane. Binds to the hydrophobic signal sequence of the ribosome-nascent chain (RNC) as it emerges from the ribosomes. The SRP-RNC complex is then targeted to the cytoplasmic membrane where it interacts with the SRP receptor FtsY. Interaction with FtsY leads to the transfer of the RNC complex to the Sec translocase for insertion into the membrane, the hydrolysis of GTP by both Ffh and FtsY, and the dissociation of the SRP-FtsY complex into the individual components.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Part of the signal recognition particle protein translocation system, which is composed of SRP and FtsY. SRP is a ribonucleoprotein composed of Ffh and a 4.5S RNA molecule.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
        <text evidence="7">The SRP-RNC complex is targeted to the cytoplasmic membrane.</text>
    </comment>
    <comment type="domain">
        <text evidence="7">Composed of three domains: the N-terminal N domain, which is responsible for interactions with the ribosome, the central G domain, which binds GTP, and the C-terminal M domain, which binds the RNA and the signal sequence of the RNC.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the GTP-binding SRP family. SRP54 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09966.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517594.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3094"/>
    <dbReference type="EnsemblBacteria" id="ABF09966">
        <property type="protein sequence ID" value="ABF09966"/>
        <property type="gene ID" value="Rmet_3094"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3094"/>
    <dbReference type="eggNOG" id="COG0541">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009301_6_0_4"/>
    <dbReference type="OMA" id="DTAGRHK"/>
    <dbReference type="OrthoDB" id="656414at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048500">
        <property type="term" value="C:signal recognition particle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008312">
        <property type="term" value="F:7S RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006614">
        <property type="term" value="P:SRP-dependent cotranslational protein targeting to membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00306">
        <property type="entry name" value="SRP54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036891">
        <property type="entry name" value="Signal_recog_part_SRP54_M_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013822">
        <property type="entry name" value="Signal_recog_particl_SRP54_hlx"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004125">
        <property type="entry name" value="Signal_recog_particle_SRP54_M"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004780">
        <property type="entry name" value="SRP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022941">
        <property type="entry name" value="SRP54"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000897">
        <property type="entry name" value="SRP54_GTPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042101">
        <property type="entry name" value="SRP54_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11564">
        <property type="entry name" value="PTHR11564"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11564:SF7">
        <property type="entry name" value="PTHR11564:SF7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00448">
        <property type="entry name" value="SRP54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02881">
        <property type="entry name" value="SRP54_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02978">
        <property type="entry name" value="SRP_SPB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00962">
        <property type="entry name" value="SRP54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00963">
        <property type="entry name" value="SRP54_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47446">
        <property type="entry name" value="SSF47446"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00959">
        <property type="entry name" value="ffh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00300">
        <property type="entry name" value="SRP54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 7" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="2 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 7" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3 7" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="5 7" id="KW-0733">Signal recognition particle</keyword>
    <feature type="domain" description="SRP54" evidence="8">
        <location>
            <begin position="276"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="114"/>
            <end position="121"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="197"/>
            <end position="201"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="255"/>
            <end position="258"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005450"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS00300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ABF09966.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="463" mass="50162" checksum="43219CDBDCC47E05" modified="2006-05-30" version="1">MLDNLTQRLARVVKTMRGEARLTEANTAEMLREVRLAMLEADVALPVVREFIARVKEKALGEDVVTSLTPGQALVGVVQRELTAVIGGDEAVADQKSNELNLNVQPPAVILMAGLQGAGKTTTSGKLAKWLKENKKKKVLTVSCDVYRPAAIAQLKTVSEQVGVDFFPSQPDQKPVEIARAALDWARKHYHDVLIVDTAGRLGIDEAMMQEIAALHAELKPAETLFVVDAMLGQDAVNTAKAFNDALPLTGVVLTKLDGDARGGAALSVRHITGRPIKFVGVGEKLDGLEPFYADRMAQRILGMGDILALVEEAQRGVDMEAAEKLAKKIKKTGGFDLEDFKAQIGQMKKMGGLGSLVDKLPAQFAAQAQGANMDQAEKQVRRMEGIINSMTPAERAKPDLIKASRKRRIAAGAGVPVQEVNRLLNQFDQMQGMMKKLKGGGMMKMMRQMGAMKGGMKGLFNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="114" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LIR4</accession>
    <name>Q1LIR4_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Polygalacturonase histidine response regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">pehR</name>
        <name evidence="10" type="ordered locus">Rmet_3090</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09962.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517590.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3090"/>
    <dbReference type="EnsemblBacteria" id="ABF09962">
        <property type="protein sequence ID" value="ABF09962"/>
        <property type="gene ID" value="Rmet_3090"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3090"/>
    <dbReference type="eggNOG" id="COG2204">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_0_6_4"/>
    <dbReference type="OMA" id="QAPIYIS"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002197">
        <property type="entry name" value="HTH_Fis"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002078">
        <property type="entry name" value="Sigma_54_int"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025943">
        <property type="entry name" value="Sigma_54_int_dom_ATP-bd_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025944">
        <property type="entry name" value="Sigma_54_int_dom_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02954">
        <property type="entry name" value="HTH_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00158">
        <property type="entry name" value="Sigma54_activat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01590">
        <property type="entry name" value="HTHFIS"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00676">
        <property type="entry name" value="SIGMA54_INTERACT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00688">
        <property type="entry name" value="SIGMA54_INTERACT_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50045">
        <property type="entry name" value="SIGMA54_INTERACT_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Response regulatory" evidence="9">
        <location>
            <begin position="10"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="domain" description="Sigma-54 factor interaction" evidence="8">
        <location>
            <begin position="162"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="128"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="540"/>
            <end position="560"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="129"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="6">
        <location>
            <position position="59"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50045"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ABF09962.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="560" mass="59896" checksum="C312ACED8124F92E" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LJI6</accession>
    <name>Q1LJI6_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Probable membrane transporter protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Rmet_2817</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cell membrane</location>
            <topology evidence="5">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the 4-toluene sulfonate uptake permease (TSUP) (TC 2.A.102) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09690.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008644115.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2817"/>
    <dbReference type="EnsemblBacteria" id="ABF09690">
        <property type="protein sequence ID" value="ABF09690"/>
        <property type="gene ID" value="Rmet_2817"/>
    </dbReference>
    <dbReference type="GeneID" id="60820732"/>
    <dbReference type="KEGG" id="rme:Rmet_2817"/>
    <dbReference type="eggNOG" id="COG0730">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045498_1_0_4"/>
    <dbReference type="OMA" id="MTPILLW"/>
    <dbReference type="OrthoDB" id="1445898at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002781">
        <property type="entry name" value="TM_pro_TauE-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01925">
        <property type="entry name" value="TauE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="33"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="71"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="102"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="143"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="188"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="213"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="238"/>
            <end position="256"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU363041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABF09690.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="257" mass="26636" checksum="E5B5A958D88B8238" modified="2006-05-30" version="1">MSLAYTVSGLLVGLLVGLTGVGGGSLMTPLLTLLFGFSPATAVGTDLAFASITKGFGTLAHRAHGHVQWQVVRRLCVGSLPAAVAAILVLKSAGELNAQWLHAIRVTIGVSVLLTVLSLLFRKQMLAWLARNPRFQLEGRKQVVATVLVGAVIGVLVTVSSIGAGAVGATLILLLYPHMKPAEVAGTDIAYAVPLTAVAGLGHVWLGTVDWNLLLALLVGSIPGIWLGAQLSRALPERIVRAALATTLTLVAIKLVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="54" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LK17</accession>
    <name>Q1LK17_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_2632</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09509.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008649808.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2632"/>
    <dbReference type="EnsemblBacteria" id="ABF09509">
        <property type="protein sequence ID" value="ABF09509"/>
        <property type="gene ID" value="Rmet_2632"/>
    </dbReference>
    <dbReference type="GeneID" id="60820932"/>
    <dbReference type="KEGG" id="rme:Rmet_2632"/>
    <dbReference type="eggNOG" id="ENOG5032ZIZ">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_176120_0_0_4"/>
    <dbReference type="OMA" id="VRLECEP"/>
    <dbReference type="OrthoDB" id="1938090at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF09509.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="106" mass="11795" checksum="FA07FD664BDE6DCC" modified="2006-05-30" version="1">MARTLTVVFGSGKEFEFTLGDSELAAVTEDAAWRWFDREYAELDCQASSPVGKVLVIDKILNVAKFSGENRFATHPEWAQDYARHAGRLLDRDKVRVDVGSAAIGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-10-07" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LK86</accession>
    <name>Q1LK86_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_2563</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09440.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF09440">
        <property type="protein sequence ID" value="ABF09440"/>
        <property type="gene ID" value="Rmet_2563"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2563"/>
    <dbReference type="HOGENOM" id="CLU_2207898_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF09440.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="107" mass="11440" checksum="60C36F68F276FE24" modified="2006-05-30" version="1">MPSMFPVIPPMTRTSIRTTRTVEENLAMATMTAMPGPSGKPTLPKAGSAGLVVVRPVRASMLALWSIGSKAGHASHVRDTSRYLFGLFAPQQNIRCSAILRAVSFAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-10-07" version="63" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LKD8</accession>
    <name>Q1LKD8_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_2511</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09388.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2511"/>
    <dbReference type="EnsemblBacteria" id="ABF09388">
        <property type="protein sequence ID" value="ABF09388"/>
        <property type="gene ID" value="Rmet_2511"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2511"/>
    <dbReference type="eggNOG" id="COG0697">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_056500_0_1_4"/>
    <dbReference type="OMA" id="WFGRERP"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000620">
        <property type="entry name" value="EamA_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00892">
        <property type="entry name" value="EamA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="42"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="78"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="106"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="133"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="161"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="191"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="224"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="282"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="domain" description="EamA" evidence="2">
        <location>
            <begin position="15"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="domain" description="EamA" evidence="2">
        <location>
            <begin position="163"/>
            <end position="296"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF09388.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="327" mass="33717" checksum="9AB6AABDF2843D79" modified="2006-05-30" version="1">MSIMKDPNHSPHSGGMLLGFIGVAIFSQTLPFTRMAVAELDATFVALGRAVIAAALALALLAMRGALASGRRPRGGQWLRLAVTALGVVVGFPVFSSLAMREVPSGHGAIVIGLLPLATAVFAAWFGNERPSLGFWLSALAGSALVVAFALWQGAGGLQHADWYLFLAVVLGALGYAEGGKLSRELGGLETISWALVVSLPVLAPVVAWLGLEHAASIAAASPRAWLGMAYVSVFSMFIGFLFWYAGLAKGGVARVGQIQLLQPFLTLAGGALILAEPLDAATVAFAVAVIAVVALGRRAAVRNTAPAMPLASAAEEAPPILPGRLH</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LKE0</accession>
    <name>Q1LKE0_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Alanyl-tRNA synthetase</fullName>
            <ecNumber evidence="7">6.1.1.7</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Rmet_2509</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-II aminoacyl-tRNA synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.7" evidence="7"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09386.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517085.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2509"/>
    <dbReference type="EnsemblBacteria" id="ABF09386">
        <property type="protein sequence ID" value="ABF09386"/>
        <property type="gene ID" value="Rmet_2509"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2509"/>
    <dbReference type="eggNOG" id="COG2872">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004485_3_2_4"/>
    <dbReference type="OMA" id="LMRLHTA"/>
    <dbReference type="OrthoDB" id="783542at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004813">
        <property type="term" value="F:alanine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006419">
        <property type="term" value="P:alanyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018165">
        <property type="entry name" value="Ala-tRNA-synth_IIc_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018164">
        <property type="entry name" value="Ala-tRNA-synth_IIc_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018163">
        <property type="entry name" value="Thr/Ala-tRNA-synth_IIc_edit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012947">
        <property type="entry name" value="tRNA_SAD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01411">
        <property type="entry name" value="tRNA-synt_2c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07973">
        <property type="entry name" value="tRNA_SAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00863">
        <property type="entry name" value="tRNA_SAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="SSF50447"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55186">
        <property type="entry name" value="SSF55186"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50860">
        <property type="entry name" value="AA_TRNA_LIGASE_II_ALA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="2 7" id="KW-0436">Ligase</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="AA_TRNA_LIGASE_II_ALA" evidence="6">
        <location>
            <begin position="1"/>
            <end position="238"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABF09386.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="238" mass="25491" checksum="CC4C59FC12671DE3" modified="2006-05-30" version="1">MPATTQKRFDDDAYLDHCQATVLAVSEAGIELDQTVCYARSGGQAGDTGTLTTADGRIVTLADTVYADDRQRILHVPASDAPALQAGDRVEVKIDWTRRHRLMRLHTCLHLLGSLIPVPVTGCGISPDSARIDFDLPESTLDKAVLTEQLNALINAKTPVTIDRITPEELAAQPDLVRTIGAAPPAGTSTIRIIEIPGVDRQPCGGTHVANTGEIGAVAVTKIEKKSRTNRRVVVNFA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="58" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LKF0</accession>
    <name>Q1LKF0_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_2499</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09376.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517077.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2499"/>
    <dbReference type="EnsemblBacteria" id="ABF09376">
        <property type="protein sequence ID" value="ABF09376"/>
        <property type="gene ID" value="Rmet_2499"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2499"/>
    <dbReference type="eggNOG" id="ENOG502ZHJX">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1650725_0_0_4"/>
    <dbReference type="OMA" id="NAKTDER"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="15"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="81"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="15"/>
            <end position="47"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF09376.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="160" mass="15569" checksum="8D8ACD5516023703" modified="2006-05-30" version="1">MTSITSVTPQVFTASGAAVTTSASQDGTDSTGKASAQDSSASANGRVNGAAFSGKAGGAGGAAAAGGTDSSSSSDTESAVIKAIKQQIETLQKLLATQMQQLQAAQNAKTDERTKASTVATLQGAVSSTNAALSQAYAKLAEAMQQESGSSTGGVVNTTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="68" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LKU1</accession>
    <name>Q1LKU1_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative membrane protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_2358</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09235.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_003149765.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2358"/>
    <dbReference type="EnsemblBacteria" id="ABF09235">
        <property type="protein sequence ID" value="ABF09235"/>
        <property type="gene ID" value="Rmet_2358"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2358"/>
    <dbReference type="eggNOG" id="COG3772">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047520_0_0_4"/>
    <dbReference type="OMA" id="TWASPQQ"/>
    <dbReference type="OrthoDB" id="364939at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021204">
        <property type="entry name" value="Integr_conj_element_PFL4711"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03755">
        <property type="entry name" value="conj_TIGR03755"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193534" evidence="2">
        <location>
            <begin position="36"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="432"/>
            <end position="464"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF09235.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="464" mass="49255" checksum="8FC3097174BC80AE" modified="2006-05-30" version="1">MKRPEPKNLSTKACRLLRPTALAGTLALVCGLAWAQVGYQNSGPVIGDDVMYSIGGGSAVSMGRAAGMRSIGVGVGWNSNLICGDMSIQTTLRNQLNGITNGFQQIMSNVIQSATSAVASLPALIIQRADPGLYNLLTNGVLQARLDFDRSKLTCRAMAEKMAETAGGQLGWSQMAEGMALRDAVGSNDAVSAVEQAETRRGNDGVPWVGGSNAGGAGQSAIRVVGDVTRAGYNLVNGRGVTDTSSIASASCASLSCQTWTSPQQATEWATRVLGEQVQRTCDSCTKTETVPGVGLTPLIQEEYEAKLEVLQELVSGTRTTTFENLREAGSTSLPITRGVIEALRDEPDQDLLARRLASEVALSSVLEKALLLQRTLLTGKKEPNVAANELAVKAVNHESDTLDREIRNLKTELELRRELANNSPMAIIQRHGTRAAGSRGIYEGDPVPDRLDQLQKGNPGARP</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-10-07" version="60" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LL53</accession>
    <name>Q1LL53_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_2244</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09123.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2244"/>
    <dbReference type="EnsemblBacteria" id="ABF09123">
        <property type="protein sequence ID" value="ABF09123"/>
        <property type="gene ID" value="Rmet_2244"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2244"/>
    <dbReference type="eggNOG" id="ENOG5032MZ8">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_102469_0_0_4"/>
    <dbReference type="OMA" id="LSYARWP"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025293">
        <property type="entry name" value="YfiR/HmsC-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13689">
        <property type="entry name" value="DUF4154"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193595" evidence="1">
        <location>
            <begin position="47"/>
            <end position="216"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF09123.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="216" mass="23123" checksum="441595ECFA2BF3F8" modified="2006-05-30" version="1">MSTGPLCQPPQLPYLTPNFAHRPRSALTLPLVLLLATPGTWPAAMAASTVTAAAPASAPVSAPVAPASRPEAVARMVMSLLSYARWPAERETLRLCVDTDARYGGKLMEGGSLSTGRLVQARSVDPLADTLAPECDALYMGAMTDARRKKLTADLVGKPVLVIVEEDFECEVGSMFCLTFRENQVSFRINLDAIARSGIHIHPGVLQLGRRRTPAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="52" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LL54</accession>
    <name>Q1LL54_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_2243</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09122.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008651931.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2243"/>
    <dbReference type="EnsemblBacteria" id="ABF09122">
        <property type="protein sequence ID" value="ABF09122"/>
        <property type="gene ID" value="Rmet_2243"/>
    </dbReference>
    <dbReference type="GeneID" id="60821184"/>
    <dbReference type="KEGG" id="rme:Rmet_2243"/>
    <dbReference type="HOGENOM" id="CLU_182819_0_0_4"/>
    <dbReference type="OMA" id="ANESRHN"/>
    <dbReference type="OrthoDB" id="1974820at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF09122.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="67" mass="7298" checksum="7E0325428C5049EB" modified="2006-05-30" version="1">MTTESRPQLKAFVQTAPQAGRYVWVISLVDFGGQQIRRTIVSDDTFTTSDAARVAGDAELKAMAADH</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="104" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LLG6</accession>
    <name>Q1LLG6_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DNA helicase</fullName>
            <ecNumber evidence="2">3.6.4.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">addA</name>
        <name evidence="9" type="ordered locus">Rmet_2131</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + H2O = ADP + H(+) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.12"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="3.6.4.12" evidence="2"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09010.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516842.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2131"/>
    <dbReference type="EnsemblBacteria" id="ABF09010">
        <property type="protein sequence ID" value="ABF09010"/>
        <property type="gene ID" value="Rmet_2131"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2131"/>
    <dbReference type="eggNOG" id="COG1074">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001114_0_0_4"/>
    <dbReference type="OMA" id="KQSIYRW"/>
    <dbReference type="OrthoDB" id="137860at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003678">
        <property type="term" value="F:DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014017">
        <property type="entry name" value="DNA_helicase_UvrD-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000212">
        <property type="entry name" value="DNA_helicase_UvrD/REP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038726">
        <property type="entry name" value="PDDEXK_AddAB-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011335">
        <property type="entry name" value="Restrct_endonuc-II-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014016">
        <property type="entry name" value="UvrD-like_ATP-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11070">
        <property type="entry name" value="PTHR11070"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12705">
        <property type="entry name" value="PDDEXK_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00580">
        <property type="entry name" value="UvrD-helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13361">
        <property type="entry name" value="UvrD_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52980">
        <property type="entry name" value="SSF52980"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51198">
        <property type="entry name" value="UVRD_HELICASE_ATP_BIND"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51217">
        <property type="entry name" value="UVRD_HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 9" id="KW-0347">Helicase</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UvrD-like helicase ATP-binding" evidence="7">
        <location>
            <begin position="13"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="domain" description="UvrD-like helicase C-terminal" evidence="8">
        <location>
            <begin position="497"/>
            <end position="833"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="542"/>
            <end position="570"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="787"/>
            <end position="814"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="551"/>
            <end position="570"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="796"/>
            <end position="814"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="34"/>
            <end position="41"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001665"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ABF09010.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="1187" mass="130901" checksum="45CAB96C4060A0B6" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="109" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LLZ3</accession>
    <name>Q1LLZ3_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase</fullName>
            <ecNumber evidence="7">2.7.7.60</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">4-diphosphocytidyl-2C-methyl-D-erythritol synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="7">MEP cytidylyltransferase</fullName>
            <shortName evidence="7">MCT</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7 9" type="primary">ispD</name>
        <name evidence="9" type="ordered locus">Rmet_1954</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Catalyzes the formation of 4-diphosphocytidyl-2-C-methyl-D-erythritol from CTP and 2-C-methyl-D-erythritol 4-phosphate (MEP).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 7">
            <text>2-C-methyl-D-erythritol 4-phosphate + CTP + H(+) = 4-CDP-2-C-methyl-D-erythritol + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:13429"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
            <dbReference type="ChEBI" id="CHEBI:57823"/>
            <dbReference type="ChEBI" id="CHEBI:58262"/>
            <dbReference type="EC" id="2.7.7.60"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 7">Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 2/6.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 7">Belongs to the IspD/TarI cytidylyltransferase family. IspD subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.60" evidence="7"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08833.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008651553.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1954"/>
    <dbReference type="EnsemblBacteria" id="ABF08833">
        <property type="protein sequence ID" value="ABF08833"/>
        <property type="gene ID" value="Rmet_1954"/>
    </dbReference>
    <dbReference type="GeneID" id="60821440"/>
    <dbReference type="KEGG" id="rme:Rmet_1954"/>
    <dbReference type="eggNOG" id="COG1211">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_061281_3_0_4"/>
    <dbReference type="OMA" id="ERQHSVY"/>
    <dbReference type="OrthoDB" id="1836139at2"/>
    <dbReference type="UniPathway" id="UPA00056">
        <property type="reaction ID" value="UER00093"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050518">
        <property type="term" value="F:2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019288">
        <property type="term" value="P:isopentenyl diphosphate biosynthetic process, methylerythritol 4-phosphate pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016114">
        <property type="term" value="P:terpenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02516">
        <property type="entry name" value="CDP-ME_synthetase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00108">
        <property type="entry name" value="IspD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001228">
        <property type="entry name" value="IspD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034683">
        <property type="entry name" value="IspD/TarI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018294">
        <property type="entry name" value="ISPD_synthase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01128">
        <property type="entry name" value="IspD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="SSF53448"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00453">
        <property type="entry name" value="ispD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01295">
        <property type="entry name" value="ISPD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0414">Isoprene biosynthesis</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 7 9" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7 9" id="KW-0808">Transferase</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="62"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="7">
        <location>
            <position position="45"/>
        </location>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="7">
        <location>
            <position position="52"/>
        </location>
    </feature>
    <feature type="site" description="Positions MEP for the nucleophilic attack" evidence="7">
        <location>
            <position position="195"/>
        </location>
    </feature>
    <feature type="site" description="Positions MEP for the nucleophilic attack" evidence="7">
        <location>
            <position position="251"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009789"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ABF08833.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="274" mass="28714" checksum="6ECC01EA81BB39A9" modified="2006-05-30" version="1">MAAGEGRIIECTPAGLPAGFRYSISRKSVSDRRFALIPCAGTGSRAGGPIQKQYQTVAGRPMLWYALAAFSACDAISATALVLAPDDMPLESRFGAAAFAGLRFDTAFVGGDSRHASVLAGLHHLGTMGAVDTDWVLVHDAARPGLTPAMIDTLVRAVESDDAASEPIGGILAVPVPDTLKRADAAARIGATVPREGLWQAQTPQMFRIGVLRHALEDALAAGAVVTDEASAIERLGLHPRLVNGSLRNFKVTYPEDFALADVLLRTGTPTSNT</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-10-07" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LM54</accession>
    <name>Q1LM54_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_1893</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08772.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF08772">
        <property type="protein sequence ID" value="ABF08772"/>
        <property type="gene ID" value="Rmet_1893"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1893"/>
    <dbReference type="HOGENOM" id="CLU_1601330_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF08772.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="166" mass="18731" checksum="32932EDF98588A91" modified="2006-05-30" version="1">MCGERLGRTLLQFADELGDATLPRLERLGILNRSDVLPFEPKRQTIKGGSGAFIFPQGLRKVCRFRQRSRLFVEFYLNVDEVALRDTAFSPVGRADTHQAVAIHYGNSASPFVAVDGDGNRWSRARAERLNAILRDLDGRCIARGDDLCLEYEFHATGELRYSKEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="65" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LMI2</accession>
    <name>Q1LMI2_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF1801 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_1765</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08644.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516497.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1765"/>
    <dbReference type="EnsemblBacteria" id="ABF08644">
        <property type="protein sequence ID" value="ABF08644"/>
        <property type="gene ID" value="Rmet_1765"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1765"/>
    <dbReference type="eggNOG" id="COG5649">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_131962_0_0_4"/>
    <dbReference type="OMA" id="GVPVWEH"/>
    <dbReference type="OrthoDB" id="1278667at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014922">
        <property type="entry name" value="DUF1801"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08818">
        <property type="entry name" value="DUF1801"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF1801" evidence="1">
        <location>
            <begin position="29"/>
            <end position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF08818"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF08644.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="132" mass="14637" checksum="5D52C0BE10E6070B" modified="2006-05-30" version="1">MTKSEPRESESASRLIDGRIQELGDWRGAMLARLRAVVKAADPEVIEEWKWRGVPVWSHAGILCTGETYKNVVKMTFAKGASLEDPAGLFNSSLEGNTRRAIDFHEGEEIDEPSLKALIQAAVVLNQSKSRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LMQ5</accession>
    <name>Q1LMQ5_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Flagellar transcriptional regulator FlhD</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">flhD</name>
        <name evidence="5" type="synonym">flhD1</name>
        <name evidence="5" type="ordered locus">Rmet_1690</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3 4">Functions in complex with FlhC as a master transcriptional regulator that regulates transcription of several flagellar and non-flagellar operons by binding to their promoter region. Activates expression of class 2 flagellar genes, including fliA, which is a flagellum-specific sigma factor that turns on the class 3 genes. Also regulates genes whose products function in a variety of physiological pathways.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homodimer; disulfide-linked. Forms a heterohexamer composed of two FlhC and four FlhD subunits. Each FlhC binds a FlhD dimer, forming a heterotrimer, and a hexamer assembles by dimerization of two heterotrimers.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="4">The C-terminal region contains a putative helix-turn-helix (HTH) motif, suggesting that this region may bind DNA.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the FlhD family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08571.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516420.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1690"/>
    <dbReference type="EnsemblBacteria" id="ABF08571">
        <property type="protein sequence ID" value="ABF08571"/>
        <property type="gene ID" value="Rmet_1690"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1690"/>
    <dbReference type="eggNOG" id="ENOG5031P80">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_144160_1_0_4"/>
    <dbReference type="OMA" id="QIMKIAS"/>
    <dbReference type="OrthoDB" id="2022460at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044780">
        <property type="term" value="P:bacterial-type flagellum assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045893">
        <property type="term" value="P:positive regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902208">
        <property type="term" value="P:regulation of bacterial-type flagellum assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.4000.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00725">
        <property type="entry name" value="FlhD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023559">
        <property type="entry name" value="Flagellar_FlhD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036194">
        <property type="entry name" value="FlhD_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05247">
        <property type="entry name" value="FlhD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63592">
        <property type="entry name" value="SSF63592"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0010">Activator</keyword>
    <keyword evidence="4" id="KW-1005">Bacterial flagellum biogenesis</keyword>
    <keyword evidence="1 4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4 5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="disulfide bond" description="Interchain" evidence="4">
        <location>
            <position position="65"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00025431"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABF08571.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="105" mass="11571" checksum="ADE4A5ADE43DEE02" modified="2006-05-30" version="1">MEVDDRVISISDFNLSYLLLAQRMILSHDAEAMFRLGVSQEVAEIIGGLSAAQLVKLARTNILLCAFRFDNHSLLATLANGSPQHDLQQMHMAILLSAHPLDSIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LMS3</accession>
    <name>Q1LMS3_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">MaoC-like dehydratase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_1672</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08553.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516401.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1672"/>
    <dbReference type="EnsemblBacteria" id="ABF08553">
        <property type="protein sequence ID" value="ABF08553"/>
        <property type="gene ID" value="Rmet_1672"/>
    </dbReference>
    <dbReference type="GeneID" id="60822312"/>
    <dbReference type="KEGG" id="rme:Rmet_1672"/>
    <dbReference type="eggNOG" id="COG2030">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_094876_3_3_4"/>
    <dbReference type="OMA" id="PGPGCIY"/>
    <dbReference type="OrthoDB" id="1563802at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005835">
        <property type="term" value="C:fatty acid synthase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004312">
        <property type="term" value="F:fatty acid synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006633">
        <property type="term" value="P:fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003965">
        <property type="entry name" value="Fatty_acid_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029069">
        <property type="entry name" value="HotDog_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002539">
        <property type="entry name" value="MaoC-like_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01575">
        <property type="entry name" value="MaoC_dehydratas"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01483">
        <property type="entry name" value="FASYNTHASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54637">
        <property type="entry name" value="SSF54637"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MaoC-like" evidence="1">
        <location>
            <begin position="18"/>
            <end position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01575"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF08553.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="146" mass="15784" checksum="32C044D4EF7E9E4A" modified="2006-05-30" version="1">MNTLNGYDYEDLQLGMTASYAKTITETDIELFATASGDNNAIHLDEDFAKTTPFGGRIAHGMLTASVISAALASRLPGPGSIYLSQNLRFRAPVKPGETVHVTVTVTDLQPEKRRVALSTVCMVQDKVVVDGDALIMPTSAKRREQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LMT9</accession>
    <name>Q1LMT9_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Outer membrane protein assembly factor BamE</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">bamE</name>
        <name evidence="7" type="ordered locus">Rmet_1654</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Part of the outer membrane protein assembly complex, which is involved in assembly and insertion of beta-barrel proteins into the outer membrane.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Part of the Bam complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cell outer membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the BamE family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08537.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1654"/>
    <dbReference type="EnsemblBacteria" id="ABF08537">
        <property type="protein sequence ID" value="ABF08537"/>
        <property type="gene ID" value="Rmet_1654"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1654"/>
    <dbReference type="eggNOG" id="COG2913">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_083835_1_1_4"/>
    <dbReference type="OMA" id="DRWEYVF"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009279">
        <property type="term" value="C:cell outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043165">
        <property type="term" value="P:Gram-negative-bacterium-type cell outer membrane assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051205">
        <property type="term" value="P:protein insertion into membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1450.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00925">
        <property type="entry name" value="OM_assembly_BamE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026592">
        <property type="entry name" value="BamE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037873">
        <property type="entry name" value="BamE-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007450">
        <property type="entry name" value="Lipoprotein_SmpA/OmlA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37482">
        <property type="entry name" value="PTHR37482"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04355">
        <property type="entry name" value="SmpA_OmlA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0998">Cell outer membrane</keyword>
    <keyword evidence="2 4" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0732">Signal</keyword>
    <feature type="domain" description="SmpA_OmlA" evidence="6">
        <location>
            <begin position="71"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="163"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="166"/>
            <end position="185"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00925"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF04355"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABF08537.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="185" mass="20516" checksum="0DF15AA19FE628D5" modified="2006-05-30" version="1">MHSGRHHPGLSLSLTERCPPEQNLLHMSSNYARAASYGIALALLTGCAAYDRGSLSLAHAITPYQITIEQGNFVSREAVAQLRPGMTRDQVSFLLGTPLLTDVFHADRWDYVFTFRRGRRDIVQQRRLTAYFAGDRLLRFDSDPMPSEKELIAEIDGVRRGKVLPSANASSPTNAAEHQPPEPTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LNK8</accession>
    <name>Q1LNK8_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Isocitrate lyase</fullName>
            <ecNumber evidence="1">4.1.3.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">Isocitrase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="5">Isocitratase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">aceA</name>
        <name evidence="9" type="ordered locus">Rmet_1385</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>D-threo-isocitrate = glyoxylate + succinate</text>
            <dbReference type="Rhea" id="RHEA:13245"/>
            <dbReference type="ChEBI" id="CHEBI:15562"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:36655"/>
            <dbReference type="EC" id="4.1.3.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="8">Can also use Mn(2+) ion.</text>
    </comment>
    <dbReference type="EC" id="4.1.3.1" evidence="1"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08268.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008649123.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1385"/>
    <dbReference type="EnsemblBacteria" id="ABF08268">
        <property type="protein sequence ID" value="ABF08268"/>
        <property type="gene ID" value="Rmet_1385"/>
    </dbReference>
    <dbReference type="GeneID" id="60822141"/>
    <dbReference type="KEGG" id="rme:Rmet_1385"/>
    <dbReference type="eggNOG" id="COG2224">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019214_2_0_4"/>
    <dbReference type="OMA" id="LEKDWAE"/>
    <dbReference type="OrthoDB" id="152545at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004451">
        <property type="term" value="F:isocitrate lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019752">
        <property type="term" value="P:carboxylic acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00377">
        <property type="entry name" value="ICL_PEPM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039556">
        <property type="entry name" value="ICL/PEPM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006254">
        <property type="entry name" value="Isocitrate_lyase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018523">
        <property type="entry name" value="Isocitrate_lyase_ph_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015813">
        <property type="entry name" value="Pyrv/PenolPyrv_Kinase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040442">
        <property type="entry name" value="Pyrv_Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21631">
        <property type="entry name" value="PTHR21631"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00463">
        <property type="entry name" value="ICL"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001362">
        <property type="entry name" value="Isocit_lyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51621">
        <property type="entry name" value="SSF51621"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01346">
        <property type="entry name" value="isocit_lyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00161">
        <property type="entry name" value="ISOCITRATE_LYASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-0456">Lyase</keyword>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Substrate binding" evidence="7">
        <location>
            <begin position="89"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="7">
        <location>
            <begin position="189"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="7">
        <location>
            <begin position="310"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="6">
        <location>
            <position position="188"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="150"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="225"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="344"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00017446"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023531"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00031022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00031921"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR001362-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR001362-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR001362-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ABF08268.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="431" mass="47300" checksum="9D57AB72353ABEDD" modified="2006-05-30" version="1">MTSRQQQIEALQKDWDTNPRWKGVKRNFTAEDVVRLRGSVQIEHTLARRGAEKLWHLLNTEPFVNTLGALTGNQAMQQVKAGLKAIYLSGWQVAGDANLAGEMYPDQSLYPANSVPMVVKRINNTFQRADQIQWSEGKGDIDYFAPIVADAEAGFGGVLNAFELMKSMIDAGAAGVHFEDQLASVKKCGHMGGKVLVPTREAVAKLTAARLAADVSGVPTLVIARTDAEAADLVTSDVDERDQPFLTGERTVEGFFRSKPGIEQSIARALAYAEVADLVWCETGKPDLEFAKRFAQAVHAKFPGKLLAYNCSPSFNWKKNLDDATIAKFQKELGAMGYKFQFITLAGFHSLNYSMFNLAHGYARNQMSAFVELQEAEFAAAEKGFTAVKHQREVGTGYFDAVTQTIEGGQSSTTALKGSTEDEQFFEKKVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="105" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LNT3</accession>
    <name>Q1LNT3_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Alcohol dehydrogenase GroES-like protein (Belongs to CMGI-2)</fullName>
            <ecNumber evidence="4">1.1.1.14</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Rmet_1308</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the zinc-containing alcohol dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.14" evidence="4"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08193.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516074.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1308"/>
    <dbReference type="EnsemblBacteria" id="ABF08193">
        <property type="protein sequence ID" value="ABF08193"/>
        <property type="gene ID" value="Rmet_1308"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1308"/>
    <dbReference type="eggNOG" id="COG1063">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026673_11_3_4"/>
    <dbReference type="OMA" id="IRYTTGC"/>
    <dbReference type="OrthoDB" id="787596at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003939">
        <property type="term" value="F:L-iditol 2-dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013149">
        <property type="entry name" value="ADH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013154">
        <property type="entry name" value="ADH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027399">
        <property type="entry name" value="ADH_N_assoc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002328">
        <property type="entry name" value="ADH_Zn_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020843">
        <property type="entry name" value="PKS_ER"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08240">
        <property type="entry name" value="ADH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13823">
        <property type="entry name" value="ADH_N_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00107">
        <property type="entry name" value="ADH_zinc_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00829">
        <property type="entry name" value="PKS_ER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="SSF50129"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00059">
        <property type="entry name" value="ADH_ZINC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1 4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="PKS_ER" evidence="3">
        <location>
            <begin position="8"/>
            <end position="351"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU361277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF08193.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="353" mass="38129" checksum="500725227180CD68" modified="2006-05-30" version="1">MKAAVYYGPQDIRCTDIPDPVIRSDHEMLVKVTATSICGSDLHLYRGALDGIMEKGKSQTGHELIGEVVEVGKSVGRFKQGDRVSMGYSVSCGHCYMCEVGQTAHCETTKNAVYGFGIPFGSINGTHAEALIVPHADGHAMNVPKGIPDEAAVTLSCNLPSAIIANRLADIQVGENVALVGCGPTGMMTLDIALHRGPGRVVVLDKVAHRLDVVRKKGGVAIDANQEDWKEKALAETGGRGFDKVIEVVGYPETLQMCLDLVRPGGTVAAIGVFCDSTFNLNLADVFLRNISLHMNGFANAQPYMWEALRLMERGVINPQEYFSHAFELADVDKAFSVFHQKSDSAMKVLIRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LNU4</accession>
    <name>Q1LNU4_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">HoxG hydrogenase 1, large subunit Uptake hydrogenase large subunit (Hydrogenlyase) (Membrane-bound hydrogenase large subunit) (Belongs to CMGI-2)</fullName>
            <ecNumber evidence="5">1.12.99.6</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">hoxG</name>
        <name evidence="5" type="ordered locus">Rmet_1297</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Ni(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:49786"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 4">Belongs to the [NiFe]/[NiFeSe] hydrogenase large subunit family.</text>
    </comment>
    <dbReference type="EC" id="1.12.99.6" evidence="5"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08182.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516064.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1297"/>
    <dbReference type="EnsemblBacteria" id="ABF08182">
        <property type="protein sequence ID" value="ABF08182"/>
        <property type="gene ID" value="Rmet_1297"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1297"/>
    <dbReference type="eggNOG" id="COG0374">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030087_0_0_4"/>
    <dbReference type="OMA" id="EEVTHSW"/>
    <dbReference type="OrthoDB" id="1967820at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008901">
        <property type="term" value="F:ferredoxin hydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033748">
        <property type="term" value="F:hydrogenase (acceptor) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016151">
        <property type="term" value="F:nickel cation binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.645.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001501">
        <property type="entry name" value="Ni-dep_hyd_lsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018194">
        <property type="entry name" value="Ni-dep_hyd_lsu_Ni_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029014">
        <property type="entry name" value="NiFe-Hase_large"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00374">
        <property type="entry name" value="NiFeSe_Hases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56762">
        <property type="entry name" value="SSF56762"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00507">
        <property type="entry name" value="NI_HGENASE_L_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00508">
        <property type="entry name" value="NI_HGENASE_L_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0456">Lyase</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0533">Nickel</keyword>
    <keyword evidence="3 4 5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009292"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003896"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABF08182.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="619" mass="69080" checksum="582AC7862E85755C" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="53" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LP32</accession>
    <name>Q1LP32_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_1208</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08094.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008648602.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1208"/>
    <dbReference type="EnsemblBacteria" id="ABF08094">
        <property type="protein sequence ID" value="ABF08094"/>
        <property type="gene ID" value="Rmet_1208"/>
    </dbReference>
    <dbReference type="GeneID" id="60822079"/>
    <dbReference type="KEGG" id="rme:Rmet_1208"/>
    <dbReference type="eggNOG" id="ENOG5033G0C">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_184979_0_0_4"/>
    <dbReference type="OMA" id="SNHAFSA"/>
    <dbReference type="OrthoDB" id="1935544at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF08094.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="88" mass="9488" checksum="C4B239586D2C73E6" modified="2006-05-30" version="1">MSANQETLAAMRTALDAHVAGSLPVGDLIEQWRASASSLTLPPVFGQAMEELLRRMEMSAVFAQDSCSFSSTAVTDQLKKWLDKAGTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="64" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LP96</accession>
    <name>Q1LP96_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_1144</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08030.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515932.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1144"/>
    <dbReference type="EnsemblBacteria" id="ABF08030">
        <property type="protein sequence ID" value="ABF08030"/>
        <property type="gene ID" value="Rmet_1144"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1144"/>
    <dbReference type="eggNOG" id="COG3381">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_077650_4_1_4"/>
    <dbReference type="OMA" id="PYASMYI"/>
    <dbReference type="OrthoDB" id="1995553at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020945">
        <property type="entry name" value="DMSO/NO3_reduct_chaperone"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036411">
        <property type="entry name" value="TorD-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02613">
        <property type="entry name" value="Nitrate_red_del"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89155">
        <property type="entry name" value="SSF89155"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF08030.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="230" mass="24200" checksum="D02A5F7554793814" modified="2006-05-30" version="1">MTSATEDSGVPDVTVAPVLEWIAGLLVAPPTGNQIARLRSPEGCAVLGAIAAEWSSHAAMLQIRRALDPCKPADTVAADLSVSYTRLFEGVCGIPAVSLYESTYAVAAREAPAQPRLYGRAAGEMDALLRQFRMGLGATREASDHISIELALLAALLRKGHIGGEVLMRARLAGWMPALIEGCIALDPDGFYGGLARLLGNLNLLEALGPDGRPRLVSVIDGQDGSHHVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LPK8</accession>
    <name>Q1LPK8_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_1032</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07918.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF07918">
        <property type="protein sequence ID" value="ABF07918"/>
        <property type="gene ID" value="Rmet_1032"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1032"/>
    <dbReference type="eggNOG" id="ENOG502ZUZ5">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_068446_0_0_4"/>
    <dbReference type="OMA" id="WITQLQL"/>
    <dbReference type="OrthoDB" id="1381369at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009279">
        <property type="term" value="C:cell outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011250">
        <property type="entry name" value="OMP/PagP_b-brl"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56925">
        <property type="entry name" value="SSF56925"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0998">Cell outer membrane</keyword>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF07918.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="348" mass="38991" checksum="A6AF50566B2B25CD" modified="2006-05-30" version="1">MARFLCWPLVMLLSEVLSFLSHRLRISVLLLSATAWMTPVHGQEVLVLGGLQRSDQGGESSYGYTYSYQHNLSENWYASFSYLNEGHIPDHHRDGHSVQLWWRYPFADRNFNVAVGIGPYRYFDTTSRSSGNGSDNDHGWGALASLSATWYVNGGPWMVQARYNRAQTRSSIKTNTLLLGVGYQLDRGDRGGPVVPPPSRTNNVTQNEFTVFLGGTIVNTFNSETSFAKAIEYRRGLSRYLSGTVSYINEGQNAALRRNGVAAQLWVERAFFNDKLTLGVGAGPYYAIDLSDHGQAAPETPSRWSGLMTMSASYRINQQWSARLSWNRTVTGYDRDTDIIMGGIGYRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LPL6</accession>
    <name>Q1LPL6_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transcriptional regulator of iron sulfur clusterassembly</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">iscR</name>
        <name evidence="2" type="ordered locus">Rmet_1024</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07910.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008649299.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1024"/>
    <dbReference type="EnsemblBacteria" id="ABF07910">
        <property type="protein sequence ID" value="ABF07910"/>
        <property type="gene ID" value="Rmet_1024"/>
    </dbReference>
    <dbReference type="GeneID" id="60821885"/>
    <dbReference type="KEGG" id="rme:Rmet_1024"/>
    <dbReference type="eggNOG" id="COG1959">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_107144_0_0_4"/>
    <dbReference type="OMA" id="YGLTIMM"/>
    <dbReference type="OrthoDB" id="1794390at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003690">
        <property type="term" value="F:double-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010242">
        <property type="entry name" value="TF_HTH_IscR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000944">
        <property type="entry name" value="Tscrpt_reg_Rrf2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33221">
        <property type="entry name" value="PTHR33221"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02082">
        <property type="entry name" value="Rrf2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02010">
        <property type="entry name" value="IscR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00738">
        <property type="entry name" value="rrf2_super"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51197">
        <property type="entry name" value="HTH_RRF2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="149"/>
            <end position="184"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF07910.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="184" mass="20148" checksum="CFFB5DF5C8E61046" modified="2006-05-30" version="1">MRLTTKGRFAVTAMIDLAMRQDQGPVTLAGISQRQKISLSYLEQLFGKLRRHEIVESVRGPGGGYSLARKAEDVTVADIIIAVDEPLDATQCGGKGNCNGDDGSGRCMTHELWATLNQKMVEYLDSVSLKNLVDQQRARQPAVLHDMREDAAQQPVAVSRGKADKQEKPARPRVVNSVFSLAQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LPN8</accession>
    <name>Q1LPN8_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Ribosome-associated heat shock protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">hslR</name>
        <name evidence="5" type="ordered locus">Rmet_1002</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the HSP15 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07888.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515802.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1002"/>
    <dbReference type="EnsemblBacteria" id="ABF07888">
        <property type="protein sequence ID" value="ABF07888"/>
        <property type="gene ID" value="Rmet_1002"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1002"/>
    <dbReference type="eggNOG" id="COG1188">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_101003_2_1_4"/>
    <dbReference type="OMA" id="IDKYLWC"/>
    <dbReference type="OrthoDB" id="2006597at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043023">
        <property type="term" value="F:ribosomal large subunit binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003727">
        <property type="term" value="F:single-stranded RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034605">
        <property type="term" value="P:cellular response to heat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00165">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.290.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025708">
        <property type="entry name" value="HSP15"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002942">
        <property type="entry name" value="S4_RNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036986">
        <property type="entry name" value="S4_RNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01479">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016821">
        <property type="entry name" value="HSP15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00363">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0346">Stress response</keyword>
    <feature type="domain" description="S4" evidence="4">
        <location>
            <begin position="11"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="88"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="93"/>
            <end position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008396"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABF07888.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="131" mass="15054" checksum="E8A93A16E24B2CA3" modified="2006-05-30" version="1">MQVDFEPDARLRIDKWLWCARFFKTRSLAAEAVERGRVSVNGQPCKNSREVKPGDTVILEAHQQQWEVVVKGVAASRGPAPIAQTLYAETEESQARRQTDAERRRFQHEPAAQMHGRPTKRDRRRIDGFKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LPR9</accession>
    <name>Q1LPR9_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Extra-Cytoplasmic Solute Receptor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">bug</name>
        <name evidence="4" type="ordered locus">Rmet_0971</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Periplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the UPF0065 (bug) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07857.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515775.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0971"/>
    <dbReference type="EnsemblBacteria" id="ABF07857">
        <property type="protein sequence ID" value="ABF07857"/>
        <property type="gene ID" value="Rmet_0971"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0971"/>
    <dbReference type="eggNOG" id="COG3181">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045683_0_1_4"/>
    <dbReference type="OMA" id="MLAYSPH"/>
    <dbReference type="OrthoDB" id="447734at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042597">
        <property type="term" value="C:periplasmic space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005064">
        <property type="entry name" value="BUG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042100">
        <property type="entry name" value="Bug_dom1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006311">
        <property type="entry name" value="TAT_signal"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42928">
        <property type="entry name" value="PTHR42928"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03401">
        <property type="entry name" value="TctC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017082">
        <property type="entry name" value="YflP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51318">
        <property type="entry name" value="TAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0675">Receptor</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193616" evidence="3">
        <location>
            <begin position="34"/>
            <end position="333"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF07857.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="333" mass="34727" checksum="A1CC9B93DC508A98" modified="2006-05-30" version="1">MTSRRQFLGQASGLAALGTLGTAGILAPTSAWAEDKWPSRPIRLVVPYPAGGSSDIIARLISKQLGEALGQPIVVDNRPGANGNIGAAMVAQAGTDNHTLLLCDVGALAISPSVYTKLTFDPNKDLKGVAMLAYSPHLLVVHPSVQVSNLKELVALSHKQTLNFAVTAIGSAPHLAGVAVQQATGAAWQYVPYKGGSQAISDTVGGTAQVLMNGMLATWPQVQAGKLKLLAVSKRSRMALVSQTPTIAESGVANFESGTWQGVMAPAGMPDAIVSRINAELIRIIRTPEIRAQLAGQGAEVVTMSPAETGKWFGTEEKRWAQVVKAAGVKLDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LPS1</accession>
    <name>Q1LPS1_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Transcriptional regulator, LysR-family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_0969</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the LysR transcriptional regulatory family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07855.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515773.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0969"/>
    <dbReference type="EnsemblBacteria" id="ABF07855">
        <property type="protein sequence ID" value="ABF07855"/>
        <property type="gene ID" value="Rmet_0969"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0969"/>
    <dbReference type="eggNOG" id="COG0583">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039613_39_0_4"/>
    <dbReference type="OMA" id="VVCMMRA"/>
    <dbReference type="OrthoDB" id="1591366at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd08417">
        <property type="entry name" value="PBP2_Nitroaromatics_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005119">
        <property type="entry name" value="LysR_subst-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000847">
        <property type="entry name" value="Tscrpt_reg_HTH_LysR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037402">
        <property type="entry name" value="YidZ_PBP2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00126">
        <property type="entry name" value="HTH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03466">
        <property type="entry name" value="LysR_substrate"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00039">
        <property type="entry name" value="HTHLYSR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50931">
        <property type="entry name" value="HTH_LYSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH lysR-type" evidence="2">
        <location>
            <begin position="6"/>
            <end position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF07855.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="313" mass="34847" checksum="1DAD2EFEF7ED484E" modified="2006-05-30" version="1">MQLSRIDLNLFVVFDAIYSEGSITAAARQLNLTQPAVSHALGRLRTLFDDPLFERRGQGMAPTPLARSLAGEVRGALQSFARTLQDTPHFDPTNTTRRFTIGMRDALESTLLPPLMARVTAMAPHVEIAAIRFDRREMESELLAGTLDAAIDILLPVSPAIHHAAFMSDPMVVLARRDHPLVKKELTLERYLAAEHVHVSSRRRGAGLEDQALHRMGLTRRVRLRCQHYAAACRVVSCTDLLATLPLRYARIANEPYANRLLSLPFKVPSLELHLYWHAGGENDGANRWLREQLMTVMESLGSGMTDVTSAAE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-01-15" modified="2022-08-03" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LQ33</accession>
    <name>RPOZ_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DNA-directed RNA polymerase subunit omega</fullName>
            <shortName evidence="1">RNAP omega subunit</shortName>
            <ecNumber evidence="1">2.7.7.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">RNA polymerase omega subunit</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Transcriptase subunit omega</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpoZ</name>
        <name type="ordered locus">Rmet_0857</name>
    </gene>
    <organism>
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Promotes RNA polymerase assembly. Latches the N- and C-terminal regions of the beta' subunit thereby facilitating its interaction with the beta and alpha subunits.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate + RNA(n+1)</text>
            <dbReference type="Rhea" id="RHEA:21248"/>
            <dbReference type="Rhea" id="RHEA-COMP:14527"/>
            <dbReference type="Rhea" id="RHEA-COMP:17342"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61557"/>
            <dbReference type="ChEBI" id="CHEBI:140395"/>
            <dbReference type="EC" id="2.7.7.6"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta' and 1 omega subunit. When a sigma factor is associated with the core the holoenzyme is formed, which can initiate transcription.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the RNA polymerase subunit omega family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.6" evidence="1"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07743.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_006578656.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q1LQ33"/>
    <dbReference type="SMR" id="Q1LQ33"/>
    <dbReference type="STRING" id="266264.Rmet_0857"/>
    <dbReference type="EnsemblBacteria" id="ABF07743">
        <property type="protein sequence ID" value="ABF07743"/>
        <property type="gene ID" value="Rmet_0857"/>
    </dbReference>
    <dbReference type="GeneID" id="60825341"/>
    <dbReference type="KEGG" id="rme:Rmet_0857"/>
    <dbReference type="eggNOG" id="COG1758">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_125406_5_1_4"/>
    <dbReference type="OMA" id="NVDNRFQ"/>
    <dbReference type="OrthoDB" id="1864134at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000428">
        <property type="term" value="C:DNA-directed RNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed 5'-3' RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.940.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00366">
        <property type="entry name" value="RNApol_bact_RpoZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003716">
        <property type="entry name" value="DNA-dir_RNA_pol_omega"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006110">
        <property type="entry name" value="Pol_omega/Rpo6/RPB6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036161">
        <property type="entry name" value="RPB6/omega-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34476">
        <property type="entry name" value="PTHR34476"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01192">
        <property type="entry name" value="RNA_pol_Rpb6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01409">
        <property type="entry name" value="RNA_pol_Rpb6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63562">
        <property type="entry name" value="SSF63562"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00690">
        <property type="entry name" value="rpoZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0804">Transcription</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_1000005987" description="DNA-directed RNA polymerase subunit omega">
        <location>
            <begin position="1"/>
            <end position="67"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00366"/>
        </source>
    </evidence>
    <sequence length="67" mass="7439" checksum="457EA41472A8A092" modified="2006-05-30" version="1">MARITVEDCLKHIPNRFELALAATYRARQLVQGHTPKVEAKDKPTVVALREIASGQVGIEMLKKVPT</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LQ50</accession>
    <name>Q1LQ50_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">IclR-family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Rmet_0840</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07726.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0840"/>
    <dbReference type="EnsemblBacteria" id="ABF07726">
        <property type="protein sequence ID" value="ABF07726"/>
        <property type="gene ID" value="Rmet_0840"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0840"/>
    <dbReference type="eggNOG" id="COG1414">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_062618_4_0_4"/>
    <dbReference type="OMA" id="QDEFDPR"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029016">
        <property type="entry name" value="GAF-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014757">
        <property type="entry name" value="Tscrpt_reg_IclR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005471">
        <property type="entry name" value="Tscrpt_reg_IclR_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09339">
        <property type="entry name" value="HTH_IclR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00346">
        <property type="entry name" value="HTH_ICLR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51077">
        <property type="entry name" value="HTH_ICLR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51078">
        <property type="entry name" value="ICLR_ED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH iclR-type" evidence="4">
        <location>
            <begin position="28"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="domain" description="IclR-ED" evidence="5">
        <location>
            <begin position="92"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABF07726.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="271" mass="29322" checksum="407DE57A45159FEE" modified="2006-05-30" version="1">MDTKKTRSTSEGETEMRDEAVGRKTAGAQTLRRGLEILRLLARHQEEGATLADIVTESGLERPTAYRLLCSLEEERFVERNIHSKRYRLGIDAMQLGAAAAEKAPIVDLVAPLMKKLARVSGDTVFLVVPQGDFTVCLHREEGPFPVRVFTTVPGQRRLLGIGAGGLALMSAMSDDAIREVHKRKHALYAEAGMPISRLLAAAARSRRKGYAEIVDTITEGVSGVGCAFQLPFGASAAISFGAISSRMNASRRDELGALMVRELSTMNPAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LQC5</accession>
    <name>Q1LQC5_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Carbon-phosphorus lyase complex subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">phnH</name>
        <name evidence="1" type="ordered locus">Rmet_0765</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07651.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515606.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0765"/>
    <dbReference type="EnsemblBacteria" id="ABF07651">
        <property type="protein sequence ID" value="ABF07651"/>
        <property type="gene ID" value="Rmet_0765"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0765"/>
    <dbReference type="eggNOG" id="COG3625">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_115317_1_0_4"/>
    <dbReference type="OMA" id="HDTPVWL"/>
    <dbReference type="OrthoDB" id="1394725at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019634">
        <property type="term" value="P:organic phosphonate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11310">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038058">
        <property type="entry name" value="PhnH-like_sp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008772">
        <property type="entry name" value="Phosphonate_metab_PhnH"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05845">
        <property type="entry name" value="PhnH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF020680">
        <property type="entry name" value="PhnH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF159709">
        <property type="entry name" value="SSF159709"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03292">
        <property type="entry name" value="PhnH_redo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0456">Lyase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF07651.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="205" mass="21346" checksum="799856FD2A84E843" modified="2006-05-30" version="1">MPSTQSMTTAPATSLLPGFDNPVDDAQQVFRAALEAFAHPGRLETLPVTSGQPEGLSPALTALLLTLADPDTPVWLPADVPAAARAFLRFHCGCPLVDNLGEATFVCVPAGHAMPALADCAQGLPAYPDRSATLLVEVASLHAGDTLTLRGPGIETTQSLCVTGLPADFRASWRANNAGFPLGVDLLLASGDEFCAMTRTTHVED</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LR28</accession>
    <name>Q1LR28_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Multidrug efflux system, subunit C</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">mdtC</name>
        <name evidence="2" type="ordered locus">Rmet_0512</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07398.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515378.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0512"/>
    <dbReference type="EnsemblBacteria" id="ABF07398">
        <property type="protein sequence ID" value="ABF07398"/>
        <property type="gene ID" value="Rmet_0512"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0512"/>
    <dbReference type="eggNOG" id="COG0841">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002755_1_2_4"/>
    <dbReference type="OMA" id="WRGVRTD"/>
    <dbReference type="OrthoDB" id="145346at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2090.10">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027463">
        <property type="entry name" value="AcrB_DN_DC_subdom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001036">
        <property type="entry name" value="Acrflvin-R"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32063">
        <property type="entry name" value="PTHR32063"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00873">
        <property type="entry name" value="ACR_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00702">
        <property type="entry name" value="ACRIFLAVINRP"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82714">
        <property type="entry name" value="SSF82714"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="400"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="424"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="495"/>
            <end position="515"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="527"/>
            <end position="545"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="592"/>
            <end position="612"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="919"/>
            <end position="936"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="969"/>
            <end position="994"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="1014"/>
            <end position="1035"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="1047"/>
            <end position="1073"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF07398.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="1100" mass="117450" checksum="FDD1DA01849FB91F" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="106" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LRA0</accession>
    <name>Q1LRA0_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Tryptophan--tRNA ligase</fullName>
            <ecNumber evidence="2">6.1.1.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">trpS</name>
        <name evidence="9" type="ordered locus">Rmet_0440</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the class-I aminoacyl-tRNA synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.2" evidence="2"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07326.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515314.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0440"/>
    <dbReference type="EnsemblBacteria" id="ABF07326">
        <property type="protein sequence ID" value="ABF07326"/>
        <property type="gene ID" value="Rmet_0440"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0440"/>
    <dbReference type="eggNOG" id="COG0180">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029244_0_1_4"/>
    <dbReference type="OMA" id="QSHVDEH"/>
    <dbReference type="OrthoDB" id="951354at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004830">
        <property type="term" value="F:tryptophan-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006436">
        <property type="term" value="P:tryptophanyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00806">
        <property type="entry name" value="TrpRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001412">
        <property type="entry name" value="aa-tRNA-synth_I_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002305">
        <property type="entry name" value="aa-tRNA-synth_Ic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002306">
        <property type="entry name" value="Trp-tRNA-ligase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00579">
        <property type="entry name" value="tRNA-synt_1b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01039">
        <property type="entry name" value="TRNASYNTHTRP"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00233">
        <property type="entry name" value="trpS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00178">
        <property type="entry name" value="AA_TRNA_LIGASE_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8 9" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="5 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 8 9" id="KW-0436">Ligase</keyword>
    <keyword evidence="4 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6 8" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU363036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ABF07326.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="342" mass="37789" checksum="9E96210B5459EBCA" modified="2006-05-30" version="1">MTDQTNPDKRPVILTGDRPTGPLHLGHFVGSLKSRVTLQETHKQYVLLADTQAMTDNAHDPDKVRRNVLEVALDYLAVGIDPAKTTISVQSHLPALAELTLMYLNFVTVSRLERNPTIKEEIQARGFGRDIPAGFLCYPASQAADITGFKAVLVPVGEDQAPLIEQTNEIVRRVNNQVGREVLPEAAALIPKHGRLPGVDGKAKMSKSQGNAIPLGASPDQIREAVHKMYTDPNHLKVSDPGQVEGNVVFTYLDAFDPNPAEVEALKEHYRRGGLGDMVLKRRVEGVLQEMLRPIRERREQLAQDPGYVFDILKKGTAEAREITQQTLDEVRGALGMFSFPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="107" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LRA8</accession>
    <name>Q1LRA8_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Proline aminopeptidase P II</fullName>
            <ecNumber evidence="9">3.4.11.9</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">pepP</name>
        <name evidence="9" type="ordered locus">Rmet_0432</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the peptidase M24B family.</text>
    </comment>
    <dbReference type="EC" id="3.4.11.9" evidence="9"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07318.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515305.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0432"/>
    <dbReference type="MEROPS" id="M24.004"/>
    <dbReference type="EnsemblBacteria" id="ABF07318">
        <property type="protein sequence ID" value="ABF07318"/>
        <property type="gene ID" value="Rmet_0432"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0432"/>
    <dbReference type="eggNOG" id="COG0006">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017266_1_0_4"/>
    <dbReference type="OMA" id="GWADTEL"/>
    <dbReference type="OrthoDB" id="415910at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030145">
        <property type="term" value="F:manganese ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070006">
        <property type="term" value="F:metalloaminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.350.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007865">
        <property type="entry name" value="Aminopep_P_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029149">
        <property type="entry name" value="Creatin/AminoP/Spt16_NTD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036005">
        <property type="entry name" value="Creatinase/aminopeptidase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000994">
        <property type="entry name" value="Pept_M24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001131">
        <property type="entry name" value="Peptidase_M24B_aminopep-P_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05195">
        <property type="entry name" value="AMP_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00557">
        <property type="entry name" value="Peptidase_M24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01011">
        <property type="entry name" value="AMP_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53092">
        <property type="entry name" value="SSF53092"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55920">
        <property type="entry name" value="SSF55920"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00491">
        <property type="entry name" value="PROLINE_PEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0031">Aminopeptidase</keyword>
    <keyword evidence="4 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0464">Manganese</keyword>
    <keyword evidence="3 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AMP_N" evidence="8">
        <location>
            <begin position="7"/>
            <end position="146"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000590"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM01011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ABF07318.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="465" mass="51237" checksum="2A7EB466CEE95917" modified="2006-05-30" version="1">MSAPDSALLGACSDRRARVLKHLRANGGGVAIVPTAPEAMRNRDSDYPYRHDSYFYYLTGFTEPEAVLVLVAGGPGDDTNSSILFCRPKHEEREIWDGFRYGPEGARAMFGFDEAHSVEDIDKLLPPMLANRATLAYPLAATADFDARVRGWLDAVRMQGRAGVSSPSAALDVRALLDEMRLFKDAGELVTMRRAAEISAQAHVRAMRTSRHGLREYHLEAELLYEFRRHGAQSVAYNSIVATGPNACVLHYRAGPAELRDGDLCLIDAGCELDGYASDITRTFPVNGRFTPAQRELYDLVQAAQDAAIAETRAGVPYNVPHDAAVRVLAQGMLDTGLLDRNKEGTLDDVLASGSYRRFYMHRTGHWLGMDVHDVGEYRVPGPLPAGHEGERAWRPLEAGMVVTIEPGIYVRPAEDVPERYWHIGIRIEDDAVVTSGDCELITRGVPVKADEIEALMRDNGGMSR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-01-15" modified="2022-08-03" version="97" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LRB7</accession>
    <name>RUVB_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Holliday junction ATP-dependent DNA helicase RuvB</fullName>
            <ecNumber evidence="1">3.6.4.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">ruvB</name>
        <name type="ordered locus">Rmet_0423</name>
    </gene>
    <organism>
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">The RuvA-RuvB complex in the presence of ATP renatures cruciform structure in supercoiled DNA with palindromic sequence, indicating that it may promote strand exchange reactions in homologous recombination. RuvAB is a helicase that mediates the Holliday junction migration by localized denaturation and reannealing.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + H2O = ADP + H(+) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.12"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Forms a complex with RuvA.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the RuvB family.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.12" evidence="1"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07309.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515298.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q1LRB7"/>
    <dbReference type="SMR" id="Q1LRB7"/>
    <dbReference type="STRING" id="266264.Rmet_0423"/>
    <dbReference type="EnsemblBacteria" id="ABF07309">
        <property type="protein sequence ID" value="ABF07309"/>
        <property type="gene ID" value="Rmet_0423"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0423"/>
    <dbReference type="eggNOG" id="COG2255">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_055599_1_0_4"/>
    <dbReference type="OMA" id="IHRMSRP"/>
    <dbReference type="OrthoDB" id="874197at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009378">
        <property type="term" value="F:four-way junction helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009432">
        <property type="term" value="P:SOS response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00016">
        <property type="entry name" value="DNA_helic_RuvB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041445">
        <property type="entry name" value="AAA_lid_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004605">
        <property type="entry name" value="DNA_helicase_Holl-junc_RuvB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008824">
        <property type="entry name" value="RuvB-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008823">
        <property type="entry name" value="RuvB_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42848">
        <property type="entry name" value="PTHR42848"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17864">
        <property type="entry name" value="AAA_lid_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05491">
        <property type="entry name" value="RuvB_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05496">
        <property type="entry name" value="RuvB_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00635">
        <property type="entry name" value="ruvB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0227">DNA damage</keyword>
    <keyword id="KW-0233">DNA recombination</keyword>
    <keyword id="KW-0234">DNA repair</keyword>
    <keyword id="KW-0347">Helicase</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0742">SOS response</keyword>
    <feature type="chain" id="PRO_1000001454" description="Holliday junction ATP-dependent DNA helicase RuvB">
        <location>
            <begin position="1"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="73"/>
            <end position="80"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00016"/>
        </source>
    </evidence>
    <sequence length="355" mass="38967" checksum="6A3D9543FB7AE1DE" modified="2006-05-30" version="1">MIETDKLTGADRLPDRVISATPASTQEEAFERALRPKLLDEYVGQEKVRGQLDIFMHAARKRREALDHVLLFGPPGLGKTTLAHIIAREMGVNLRQTSGPVLERPGDLAALLTNLEAHDVLFIDEIHRLSPVVEEILYPALEDYQIDIMIGEGPAARSVKLDLQPFTLVGATTRAGMLTNPLRDRFGIVARLEFYTPDELARIVARSALLLNAAIDPAGALEIARRARGTPRIANRLLRRVRDFAEVKSDGTITRELADAALEMLDVDSVGFDLMDRKLLEAVLHKFDGGPVGVDNLAAAIGEARDTIEDVLEPYMIQQGYLQRTPRGRMATAAAYRHFGLASPRDGGADLAEGL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-01-15" modified="2022-08-03" version="107" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LRG2</accession>
    <name>SYDND_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Aspartate--tRNA(Asp/Asn) ligase</fullName>
            <ecNumber evidence="1">6.1.1.23</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Aspartyl-tRNA synthetase</fullName>
            <shortName evidence="1">AspRS</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Non-discriminating aspartyl-tRNA synthetase</fullName>
            <shortName evidence="1">ND-AspRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">aspS</name>
        <name type="ordered locus">Rmet_0378</name>
    </gene>
    <organism>
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Aspartyl-tRNA synthetase with relaxed tRNA specificity since it is able to aspartylate not only its cognate tRNA(Asp) but also tRNA(Asn). Reaction proceeds in two steps: L-aspartate is first activated by ATP to form Asp-AMP and then transferred to the acceptor end of tRNA(Asp/Asn).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + L-aspartate + tRNA(Asx) = AMP + diphosphate + L-aspartyl-tRNA(Asx)</text>
            <dbReference type="Rhea" id="RHEA:18349"/>
            <dbReference type="Rhea" id="RHEA-COMP:9710"/>
            <dbReference type="Rhea" id="RHEA-COMP:9711"/>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78516"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.23"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-II aminoacyl-tRNA synthetase family. Type 1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.23" evidence="1"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07264.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515256.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q1LRG2"/>
    <dbReference type="SMR" id="Q1LRG2"/>
    <dbReference type="STRING" id="266264.Rmet_0378"/>
    <dbReference type="PRIDE" id="Q1LRG2"/>
    <dbReference type="EnsemblBacteria" id="ABF07264">
        <property type="protein sequence ID" value="ABF07264"/>
        <property type="gene ID" value="Rmet_0378"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0378"/>
    <dbReference type="eggNOG" id="COG0173">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014330_3_2_4"/>
    <dbReference type="OMA" id="YQLDVEM"/>
    <dbReference type="OrthoDB" id="226836at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004815">
        <property type="term" value="F:aspartate-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050560">
        <property type="term" value="F:aspartate-tRNA(Asn) ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006422">
        <property type="term" value="P:aspartyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1360.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00044">
        <property type="entry name" value="Asp_tRNA_synth_type1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004364">
        <property type="entry name" value="Aa-tRNA-synt_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004524">
        <property type="entry name" value="Asp-tRNA-ligase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002312">
        <property type="entry name" value="Asp/Asn-tRNA-synth_IIb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004115">
        <property type="entry name" value="GAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029351">
        <property type="entry name" value="GAD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004365">
        <property type="entry name" value="NA-bd_OB_tRNA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22594:SF5">
        <property type="entry name" value="PTHR22594:SF5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02938">
        <property type="entry name" value="GAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00152">
        <property type="entry name" value="tRNA-synt_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01336">
        <property type="entry name" value="tRNA_anti-codon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01042">
        <property type="entry name" value="TRNASYNTHASP"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55261">
        <property type="entry name" value="SSF55261"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="SSF55681"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00459">
        <property type="entry name" value="aspS_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_1000006735" description="Aspartate--tRNA(Asp/Asn) ligase">
        <location>
            <begin position="1"/>
            <end position="602"/>
        </location>
    </feature>
    <feature type="region of interest" description="Aspartate" evidence="1">
        <location>
            <begin position="199"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="175"/>
        </location>
        <ligand>
            <name>L-aspartate</name>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="221"/>
            <end position="223"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="221"/>
        </location>
        <ligand>
            <name>L-aspartate</name>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="230"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="458"/>
        </location>
        <ligand>
            <name>L-aspartate</name>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="492"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="499"/>
        </location>
        <ligand>
            <name>L-aspartate</name>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="544"/>
            <end position="547"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="site" description="Important for tRNA non-discrimination" evidence="1">
        <location>
            <position position="33"/>
        </location>
    </feature>
    <feature type="site" description="Important for tRNA non-discrimination" evidence="1">
        <location>
            <position position="84"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00044"/>
        </source>
    </evidence>
    <sequence length="602" mass="68186" checksum="E586C5B7E846882B" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LRL0</accession>
    <name>Q1LRL0_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Arsenite efflux pump ACR3 CMGI-7</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">arsB</name>
        <name evidence="5" type="ordered locus">Rmet_0330</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 3">Belongs to the arsenical resistance-3 (ACR3) (TC 2.A.59) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07216.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515211.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0330"/>
    <dbReference type="DNASU" id="4037117"/>
    <dbReference type="EnsemblBacteria" id="ABF07216">
        <property type="protein sequence ID" value="ABF07216"/>
        <property type="gene ID" value="Rmet_0330"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0330"/>
    <dbReference type="eggNOG" id="COG0798">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022869_0_2_4"/>
    <dbReference type="OMA" id="ISAKWYD"/>
    <dbReference type="OrthoDB" id="1493959at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015297">
        <property type="term" value="F:antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015103">
        <property type="term" value="F:inorganic anion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1530.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004706">
        <property type="entry name" value="Arsenical-R_Acr3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002657">
        <property type="entry name" value="BilAc:Na_symport/Acr3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038770">
        <property type="entry name" value="Na+/solute_symporter_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43057">
        <property type="entry name" value="PTHR43057"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01758">
        <property type="entry name" value="SBF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005508">
        <property type="entry name" value="Acr3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00832">
        <property type="entry name" value="acr3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="3 4" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 4" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="21"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="53"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="94"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="129"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="168"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="194"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="228"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="259"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="294"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="322"/>
            <end position="344"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRNR" id="PIRNR005508"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABF07216.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="354" mass="37919" checksum="06B449247B73A3BA" modified="2006-05-30" version="1">MSTAQRTAGASQPSAIGFFERYLTVWVALCIVAGILLGQWLPAAFHTIGAMEIAQVNLPVGILIWIMIIPMLIKIDFGAMGQVAGHWRGIGVTLFVNWLVKPFSMALLGWIFVRHLFAGWLPADQLDSYIAGLILLAAAPCTAMVFVWSQLCKGDPYFTLSQVALNDAIMVVAFAPVVALLLGLSSITVPWDTLMTSVALYIVVPVLIAQVIRKVLLVRGDATYKRVVGALGPYSITALLATLVLLFGFQGNAIVEQPLIILLLAVPILIQVLLNSGLAYLLNRKLGVAHCVAGPSALIGASNFFELAVATAISLFGFQSGAALATVVGVLIEVPVMLFVVGVVNRSQHWYESH</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-10-07" version="57" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LRV5</accession>
    <name>Q1LRV5_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_0235</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07121.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0235"/>
    <dbReference type="EnsemblBacteria" id="ABF07121">
        <property type="protein sequence ID" value="ABF07121"/>
        <property type="gene ID" value="Rmet_0235"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0235"/>
    <dbReference type="eggNOG" id="ENOG50335NT">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_080357_1_0_4"/>
    <dbReference type="OMA" id="ACSPRYD"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="22"/>
            <end position="41"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF07121.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="229" mass="24192" checksum="406D7A6776654BE0" modified="2006-05-30" version="1">MVKTSPHLISCASGANVRGFPDNTGLRQIAPTSPMRRPSPRPAPRVPLLIAIVLLALAGCSPRYDWRTIVSNEGQYAALYPDKPTSAARDVAIAGRQLPMRMEAARIDNTLFAVGVVSLPADDAGLRREALASMQAGLVGNLGAHPDSPARSRPVTVMSAGQPPVALDGVEVTVAGISPQDKSPRRLTARLVASGSRVYQAVVLEAGDAAKDARQAEQVEQFLTGFHPF</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LS00</accession>
    <name>Q1LS00_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative type 4 fimbrial biogenesis transmembrane protein, pilW</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_0190</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07076.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0190"/>
    <dbReference type="EnsemblBacteria" id="ABF07076">
        <property type="protein sequence ID" value="ABF07076"/>
        <property type="gene ID" value="Rmet_0190"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0190"/>
    <dbReference type="eggNOG" id="COG4966">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_073585_0_0_4"/>
    <dbReference type="OMA" id="CTGYDGR"/>
    <dbReference type="OrthoDB" id="1209523at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043683">
        <property type="term" value="P:type IV pilus assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012902">
        <property type="entry name" value="N_methyl_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032092">
        <property type="entry name" value="PilW"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07963">
        <property type="entry name" value="N_methyl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16074">
        <property type="entry name" value="PilW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00409">
        <property type="entry name" value="PROKAR_NTER_METHYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="38"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF07076.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="242" mass="25480" checksum="230A4E4E7F9AB0CD" modified="2006-05-30" version="1">MRHLRRTRSRQAGVSLVEIMVAMIIGLFMLLALSTLFLNFRGTFRDQDQLAQLQDNERLALTMFSTTIQSAGYFPDPVNNVVTGALPLSTGNAYGDFAAGQAVVGTSGANGASDTLTARFMTANGDGILNCQGQSNTSGANVVYVNSFAVNVNNELTCAINGGAAVPLVSGVTGFSVLYGTDTANGGNVDQYLSAAAVTAAGYWPQVRTARVTVTFVNPYATQPSQPATLSWTQTISLMNRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LS38</accession>
    <name>Q1LS38_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">PPK2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Rmet_0152</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the polyphosphate kinase 2 (PPK2) family. Class I subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07038.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515065.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0152"/>
    <dbReference type="EnsemblBacteria" id="ABF07038">
        <property type="protein sequence ID" value="ABF07038"/>
        <property type="gene ID" value="Rmet_0152"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0152"/>
    <dbReference type="eggNOG" id="COG2326">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_048699_1_2_4"/>
    <dbReference type="OMA" id="WAPWYVV"/>
    <dbReference type="OrthoDB" id="953793at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008976">
        <property type="term" value="F:polyphosphate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006797">
        <property type="term" value="P:polyphosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016898">
        <property type="entry name" value="Polyphosphate_phosphotransfera"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022300">
        <property type="entry name" value="PPK2-rel_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022488">
        <property type="entry name" value="PPK2-related"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03976">
        <property type="entry name" value="PPK2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF028756">
        <property type="entry name" value="PPK2_prd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03709">
        <property type="entry name" value="PPK2_rel_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PPK2" evidence="4">
        <location>
            <begin position="31"/>
            <end position="249"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009924"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF03976"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABF07038.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="269" mass="31058" checksum="9016AD855172E886" modified="2006-05-30" version="1">MKLDDFCITSGKGFKLAAFDPESKPFSLGEKDADVAHVADLSARLDAEQDIFYAEHRRKLLFILQGMDTSGKDGTVRSVFRNFDPLGVRVASFKAPTAEELARDYLWRVHLQVPRQGEIAIFNRSHYEDVLITRVHGWIDDEECERRFRQIRAFEEMLTETGTTIIKCFLHISKAEQKRRLEDRIADPQKHWKFDMQDLAERKYWKEYQQSYEAAIAATATPACPWYIVPADSKSHRNLMVAEILADVFHKLKPSYPPPKPELAKVAVE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-04-14" modified="2022-08-03" version="95" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LS40</accession>
    <name>PYRE_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Orotate phosphoribosyltransferase</fullName>
            <shortName evidence="1">OPRT</shortName>
            <shortName evidence="1">OPRTase</shortName>
            <ecNumber evidence="1">2.4.2.10</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">pyrE</name>
        <name type="ordered locus">Rmet_0150</name>
    </gene>
    <organism>
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the transfer of a ribosyl phosphate group from 5-phosphoribose 1-diphosphate to orotate, leading to the formation of orotidine monophosphate (OMP).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>diphosphate + orotidine 5'-phosphate = 5-phospho-alpha-D-ribose 1-diphosphate + orotate</text>
            <dbReference type="Rhea" id="RHEA:10380"/>
            <dbReference type="ChEBI" id="CHEBI:30839"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57538"/>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <dbReference type="EC" id="2.4.2.10"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="1">Pyrimidine metabolism; UMP biosynthesis via de novo pathway; UMP from orotate: step 1/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the purine/pyrimidine phosphoribosyltransferase family. PyrE subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.10" evidence="1"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07036.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008647201.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q1LS40"/>
    <dbReference type="SMR" id="Q1LS40"/>
    <dbReference type="STRING" id="266264.Rmet_0150"/>
    <dbReference type="EnsemblBacteria" id="ABF07036">
        <property type="protein sequence ID" value="ABF07036"/>
        <property type="gene ID" value="Rmet_0150"/>
    </dbReference>
    <dbReference type="GeneID" id="60822873"/>
    <dbReference type="KEGG" id="rme:Rmet_0150"/>
    <dbReference type="eggNOG" id="COG0461">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_074878_0_1_4"/>
    <dbReference type="OMA" id="MKAYQRQ"/>
    <dbReference type="OrthoDB" id="1280396at2"/>
    <dbReference type="UniPathway" id="UPA00070">
        <property type="reaction ID" value="UER00119"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004588">
        <property type="term" value="F:orotate phosphoribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044205">
        <property type="term" value="P:'de novo' UMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd06223">
        <property type="entry name" value="PRTases_typeI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2020">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01208">
        <property type="entry name" value="PyrE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023031">
        <property type="entry name" value="OPRT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004467">
        <property type="entry name" value="Or_phspho_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000836">
        <property type="entry name" value="PRibTrfase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029057">
        <property type="entry name" value="PRTase-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00156">
        <property type="entry name" value="Pribosyltran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53271">
        <property type="entry name" value="SSF53271"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00336">
        <property type="entry name" value="pyrE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00103">
        <property type="entry name" value="PUR_PYR_PR_TRANSFER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0328">Glycosyltransferase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0665">Pyrimidine biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_1000138819" description="Orotate phosphoribosyltransferase">
        <location>
            <begin position="1"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="binding site" description="in other chain" evidence="1">
        <location>
            <position position="32"/>
        </location>
        <ligand>
            <name>5-phospho-alpha-D-ribose 1-diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="40"/>
            <end position="41"/>
        </location>
        <ligand>
            <name>orotate</name>
            <dbReference type="ChEBI" id="CHEBI:30839"/>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="1">
        <location>
            <begin position="78"/>
            <end position="79"/>
        </location>
        <ligand>
            <name>5-phospho-alpha-D-ribose 1-diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="104"/>
        </location>
        <ligand>
            <name>5-phospho-alpha-D-ribose 1-diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="1">
        <location>
            <position position="105"/>
        </location>
        <ligand>
            <name>5-phospho-alpha-D-ribose 1-diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="108"/>
        </location>
        <ligand>
            <name>5-phospho-alpha-D-ribose 1-diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="110"/>
        </location>
        <ligand>
            <name>5-phospho-alpha-D-ribose 1-diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="1">
        <location>
            <begin position="129"/>
            <end position="137"/>
        </location>
        <ligand>
            <name>5-phospho-alpha-D-ribose 1-diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="133"/>
        </location>
        <ligand>
            <name>orotate</name>
            <dbReference type="ChEBI" id="CHEBI:30839"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="161"/>
        </location>
        <ligand>
            <name>orotate</name>
            <dbReference type="ChEBI" id="CHEBI:30839"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01208"/>
        </source>
    </evidence>
    <sequence length="225" mass="23821" checksum="E02FB62125F48BD7" modified="2006-05-30" version="1">MTQQTTPDDLSQSFIRFALDAGVLSFGEFVTKAGRKSPYFFNAGLFNQGAMLGQVAQFYAKTLLESGVQFDVLFGPAYKGITLASATAVALAGMGRDVGFSYNRKEAKDHGEGGTLVGAKLQGKVVIIDDVISAGTSVRESMQLIRAAGAEPAAVLIALDRMEKSGTADNIGTHSAVQDVQREYGIPVIAIATLKDLLAYLDASKDPSLGNSREAVAAYRQKYGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="69" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LS89</accession>
    <name>Q1LS89_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative metallo-beta-lactamase superfamily protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_0101</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF06987.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515022.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0101"/>
    <dbReference type="EnsemblBacteria" id="ABF06987">
        <property type="protein sequence ID" value="ABF06987"/>
        <property type="gene ID" value="Rmet_0101"/>
    </dbReference>
    <dbReference type="GeneID" id="60822823"/>
    <dbReference type="KEGG" id="rme:Rmet_0101"/>
    <dbReference type="eggNOG" id="COG0491">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_048478_0_1_4"/>
    <dbReference type="OMA" id="HSPEHAC"/>
    <dbReference type="OrthoDB" id="733552at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001279">
        <property type="entry name" value="Metallo-B-lactamas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00753">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00849">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="SSF56281"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Lactamase_B" evidence="1">
        <location>
            <begin position="42"/>
            <end position="270"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF06987.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="359" mass="40290" checksum="E0F54F8CC6260012" modified="2006-05-30" version="1">MNALEHQLQYPFGETMPEPGTRQEVAPGVYWLRMPLPFALDHINLWLLRDRIDGRDGWTIVDCGITNDTIKAHWETIFANELEGLPVLRVLVTHCHPDHVGLAHWLCKHWDVRLWMSLGDYMSARVMAGGSGVGSNAGGDFAASHFARHGMVDPDNLEKLRARKSYYPSLVPDLPTQYRRLMDGDTVKIGADPATASWRVITGYGHAPEHVALFNPATNVLISGDMVLPRISTNVSVFDMEPEANPLQLYLDSLGKYEGLPKDVLILPSHGRPFRNLHTRINQLREHHVDRLAETLAACAEKPCNAHDIVSVIFRRQFDIHQLTFAIGEALAHLHALWHRGELVRVVGDDGVIRFKKAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="92" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LSA8</accession>
    <name>Q1LSA8_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Transcriptional regulator, IclR-family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Rmet_0082</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF06968.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515005.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0082"/>
    <dbReference type="EnsemblBacteria" id="ABF06968">
        <property type="protein sequence ID" value="ABF06968"/>
        <property type="gene ID" value="Rmet_0082"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0082"/>
    <dbReference type="eggNOG" id="COG1414">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_062618_2_0_4"/>
    <dbReference type="OMA" id="GLHHVHG"/>
    <dbReference type="OrthoDB" id="370329at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029016">
        <property type="entry name" value="GAF-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014757">
        <property type="entry name" value="Tscrpt_reg_IclR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005471">
        <property type="entry name" value="Tscrpt_reg_IclR_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09339">
        <property type="entry name" value="HTH_IclR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01614">
        <property type="entry name" value="IclR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00346">
        <property type="entry name" value="HTH_ICLR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51077">
        <property type="entry name" value="HTH_ICLR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51078">
        <property type="entry name" value="ICLR_ED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH iclR-type" evidence="3">
        <location>
            <begin position="22"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="domain" description="IclR-ED" evidence="4">
        <location>
            <begin position="85"/>
            <end position="271"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABF06968.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="285" mass="30778" checksum="129E27B38638A4CF" modified="2006-05-30" version="1">MTAAEDDDTRDDIADDTRRAGIQSIEVGFRLLQALAASPRAMMLRDLAAAADMSPAKAHRYLVSFQRLGAVTQDAVSQRYDLGPFALQLGLAGLNRLDPVRKARPILSQLRDEIDQTAGIAVWGNHGPTIVHWEESSHPVSASLRLGDVMPMLNSATGRLYGAYLSRRQTLPLIERELATRGTVPLPDMPVSLDEYDAICADVREHGAARTRGGVLPGINAISTPVFDANGHLAMALIVLGAQSVFDAEWGGTVDRRVRDIARQISSELGYLDGNQRAGQTGQTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LSF4</accession>
    <name>Q1LSF4_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Mg2+ and Co2+ transporter, CorA-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">corA1</name>
        <name evidence="7" type="ordered locus">Rmet_0036</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CorA metal ion transporter (MIT) (TC 1.A.35) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF06922.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0036"/>
    <dbReference type="EnsemblBacteria" id="ABF06922">
        <property type="protein sequence ID" value="ABF06922"/>
        <property type="gene ID" value="Rmet_0036"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0036"/>
    <dbReference type="eggNOG" id="COG0598">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007127_0_2_4"/>
    <dbReference type="OMA" id="IQKYFRD"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046873">
        <property type="term" value="F:metal ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045861">
        <property type="entry name" value="CorA_cytoplasmic_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045863">
        <property type="entry name" value="CorA_TM1_TM2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002523">
        <property type="entry name" value="MgTranspt_CorA/ZnTranspt_ZntB"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01544">
        <property type="entry name" value="CorA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143865">
        <property type="entry name" value="SSF143865"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144083">
        <property type="entry name" value="SSF144083"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="303"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="334"/>
            <end position="351"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009765"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABF06922.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="360" mass="40566" checksum="484C136882042094" modified="2006-05-30" version="1">MRTTGAPAARQKVVMAGMVHIKCLGLLRLHVLKYGASMGAIVNCAAYRDGRKVGDVEFDGIARALAEPGTFVWLGLHEPDLPLLRKVQQAFGLHDLAVEDALEPHQRPKIEAYGDSVFVVLGTARLVDDEVVVGETHLYVGPNYVVSVRHGASSTYTPVRERCEEDPRGMAHGTGFVLYALMDYIVDHYLPIVNRLEEAFEALEQDIFHDEFDRAAIERLYKVKHQVLRLRNVVYPVEDICSQLIRLHEDLVPKDLRAYFRDIQDHSSRVVRTLDVVREMLTTAVQVNLALVTVTQNEVVKRLAGWGAILAVPTVVFSLYGMNFNFMPELKFHYAYPVVIGFTAAACSFLWRKLHRAGWI</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="107" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LSG8</accession>
    <name>Q1LSG8_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Transcriptional regulator, AraC-family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Rmet_0022</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF06908.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011514955.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0022"/>
    <dbReference type="EnsemblBacteria" id="ABF06908">
        <property type="protein sequence ID" value="ABF06908"/>
        <property type="gene ID" value="Rmet_0022"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0022"/>
    <dbReference type="eggNOG" id="COG2169">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_87_4_4"/>
    <dbReference type="OMA" id="HELVWVR"/>
    <dbReference type="OrthoDB" id="1757088at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003313">
        <property type="entry name" value="AraC-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018060">
        <property type="entry name" value="HTH_AraC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018062">
        <property type="entry name" value="HTH_AraC-typ_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011051">
        <property type="entry name" value="RmlC_Cupin_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020449">
        <property type="entry name" value="Tscrpt_reg_HTH_AraC-type"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02311">
        <property type="entry name" value="AraC_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12833">
        <property type="entry name" value="HTH_18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00032">
        <property type="entry name" value="HTHARAC"/>
    </dbReference>
    <dbReference type="SMART" id="SM00342">
        <property type="entry name" value="HTH_ARAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51182">
        <property type="entry name" value="SSF51182"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00041">
        <property type="entry name" value="HTH_ARAC_FAMILY_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01124">
        <property type="entry name" value="HTH_ARAC_FAMILY_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH araC/xylS-type" evidence="4">
        <location>
            <begin position="172"/>
            <end position="272"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS01124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABF06908.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="272" mass="30162" checksum="B12A8017E08D348E" modified="2006-05-30" version="1">MPAEAPNAAHADGRPDTYSEQPAYLQYDRGPMPVAAMAADYLPTHVTKPHQHPHAQLIHAVHGVMVVSTSEGQWIVPPTRGMWMPGGTVHWIRMVGHVQMRTAYIRPDAAPNLPTRCTVLGISPLLRELILAAIDIPLPYSPDSRESRLMQLLLDEVTLVPTLPLHLPRPKDKDLRVICERITDSPDSGLTLADWGDRLGVDPKTIQRRFARETGMTFGQWRQQARLLAALERLAAGDKVVDVALDMGYGSPSAFATMFRRQFGVPPSAFFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-04-26" modified="2021-09-29" version="92" xmlns="http://uniprot.org/uniprot">
    <accession>Q58AF2</accession>
    <name>Q58AF2_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">SilC, OMP component, silver resistance, three components proton antiporter efflux system</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">silC</name>
        <name evidence="4" type="ordered locus">Rmet_6134</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4 5">
        <name>pMOL30</name>
    </geneLocation>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="5">Plasmid pMOL30</plasmid>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the outer membrane factor (OMF) (TC 1.B.17) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000354">
        <property type="protein sequence ID" value="ABF12993.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008642714.1">
        <property type="nucleotide sequence ID" value="NC_007971.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_145666.1">
        <property type="nucleotide sequence ID" value="NC_006466.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF12993">
        <property type="protein sequence ID" value="ABF12993"/>
        <property type="gene ID" value="Rmet_6134"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6134"/>
    <dbReference type="HOGENOM" id="CLU_012817_15_1_4"/>
    <dbReference type="OMA" id="YRQDAQN"/>
    <dbReference type="OrthoDB" id="1533572at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid pMOL30"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015562">
        <property type="term" value="F:efflux transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003423">
        <property type="entry name" value="OMP_efflux"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02321">
        <property type="entry name" value="OEP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0614">Plasmid</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5013531908" evidence="3">
        <location>
            <begin position="29"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="188"/>
            <end position="215"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF12993.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="435" mass="46854" checksum="88B718D578DBEE1A" modified="2005-04-26" version="1">MFLRFPLARGRRWSLALSLIAAAHPGWAQSAAPLSLQEAVVLASAHASDAETSRAAIQSALEMAVAGRQLPDPVLKVGVNNVPADGRDRFSIGTESMTMRSISVMQELTRSAKREARARRFEAEASSAQAQRTAALAAVQRGAAQAWLDRLYAERATVVLAEQAQSIGLMIDAARAAYRGNRGPRTDILTAELELQQLRDREDEARAAVSTARAMLQRWVGDAAVRPLGDSPTLAIPAWLANLDGSVVSQLPDVATAEAQVGVAEAEGSVARENRIPDVSVELMYSQRGPAYSNMVSVNLSLPLPWDRKNRQDRELAAKLSQADAARAQAEAVRRGLQGQLRGKRAELGLNQSRLERYRDVTVPLARTQTEAALTAYRAGAGTLSAVADASRKALNLSLERLKLEAATARLWAELNFLMPLATEAMPASTQGDQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-04-26" modified="2021-06-02" version="65" xmlns="http://uniprot.org/uniprot">
    <accession>Q58AS9</accession>
    <name>Q58AS9_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_6056</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="1 2">
        <name>pMOL30</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid pMOL30</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000354">
        <property type="protein sequence ID" value="ABF12915.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011229290.1">
        <property type="nucleotide sequence ID" value="NC_007971.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_145536.1">
        <property type="nucleotide sequence ID" value="NC_006466.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF12915">
        <property type="protein sequence ID" value="ABF12915"/>
        <property type="gene ID" value="Rmet_6056"/>
    </dbReference>
    <dbReference type="GeneID" id="60825075"/>
    <dbReference type="KEGG" id="rme:Rmet_6056"/>
    <dbReference type="HOGENOM" id="CLU_2603484_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid pMOL30"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF12915.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="79" mass="8423" checksum="6C545B7D76438CA3" modified="2005-04-26" version="1">MAIHYDNADENKGGFTSEQMNALIEGASEQQVRLALQKVLHVMAKLGFGTDEEISGADAVDAIGEVFAEVTEEVLQGQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-01-04" modified="2022-05-25" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q5NUX7</accession>
    <name>Q5NUX7_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Transposase ISRme18</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">tnpA</name>
        <name evidence="8" type="ordered locus">Rmet_6212</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="8 9">
        <name>pMOL28</name>
    </geneLocation>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="9">Plasmid pMOL28</plasmid>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the transposase 7 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000355">
        <property type="protein sequence ID" value="ABF13071.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011239964.1">
        <property type="nucleotide sequence ID" value="NC_007972.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_161703.1">
        <property type="nucleotide sequence ID" value="NC_006525.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF13071">
        <property type="protein sequence ID" value="ABF13071"/>
        <property type="gene ID" value="Rmet_6212"/>
    </dbReference>
    <dbReference type="GeneID" id="60825770"/>
    <dbReference type="KEGG" id="rme:Rmet_6212"/>
    <dbReference type="HOGENOM" id="CLU_009098_1_0_4"/>
    <dbReference type="OMA" id="VQSGHCT"/>
    <dbReference type="OrthoDB" id="45599at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid pMOL28"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004803">
        <property type="term" value="F:transposase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006313">
        <property type="term" value="P:transposition, DNA-mediated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025296">
        <property type="entry name" value="DUF4158"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002513">
        <property type="entry name" value="Tn3_Tnp_DDE_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01526">
        <property type="entry name" value="DDE_Tnp_Tn3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13700">
        <property type="entry name" value="DUF4158"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8" id="KW-0614">Plasmid</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0814">Transposable element</keyword>
    <keyword evidence="3" id="KW-0815">Transposition</keyword>
    <feature type="domain" description="DUF4158" evidence="7">
        <location>
            <begin position="12"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="domain" description="DDE_Tnp_Tn3" evidence="6">
        <location>
            <begin position="567"/>
            <end position="951"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009402"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022578"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01526"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF13700"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ABF13071.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="979" mass="110610" checksum="9E347E582F611B49" modified="2005-01-04" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2005-01-04" modified="2022-05-25" version="70" xmlns="http://uniprot.org/uniprot">
    <accession>Q5NV50</accession>
    <name>Q5NV50_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_6310</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="1 2">
        <name>pMOL28</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid pMOL28</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000355">
        <property type="protein sequence ID" value="ABF13169.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008649465.1">
        <property type="nucleotide sequence ID" value="NC_007972.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_161631.1">
        <property type="nucleotide sequence ID" value="NC_006525.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF13169">
        <property type="protein sequence ID" value="ABF13169"/>
        <property type="gene ID" value="Rmet_6310"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6310"/>
    <dbReference type="HOGENOM" id="CLU_1583757_0_0_4"/>
    <dbReference type="OMA" id="GEKYNVW"/>
    <dbReference type="OrthoDB" id="1487572at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid pMOL28"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF13169.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="168" mass="19194" checksum="E003933CD1DAAD84" modified="2005-01-04" version="1">MRSSLFREVIKQHNLSAKLSPVFTLSPDLDEICARVVDYIGTNYRVREEPLIKEMLTDGIAAWRAARKHGDPSVAFMKGLFSRAHDLYNKRYAAFKGEKYNVWYPFHESIPAFEQRQLAGYVCQVVDEPVPGEVSQRCAAFQLAARVLTGYAFSRYFESYDVAGNFAH</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-03-23" modified="2021-04-07" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>D3DXL5</accession>
    <name>D3DXL5_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_6417</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ADC45035.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515316.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_6417"/>
    <dbReference type="EnsemblBacteria" id="ADC45035">
        <property type="protein sequence ID" value="ADC45035"/>
        <property type="gene ID" value="Rmet_6417"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6417"/>
    <dbReference type="HOGENOM" id="CLU_2846685_0_0_4"/>
    <dbReference type="OrthoDB" id="1713183at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADC45035.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="65" mass="7345" checksum="1AFA3C29EE63AFBB" modified="2010-03-23" version="1">MPRNASAAVNVGPYADIRFRYAWFDNSVRGDQSPEAGDYSQEWNVSVEGAWAHGMRGERRGRRSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-03-23" modified="2020-10-07" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>D3DXX0</accession>
    <name>D3DXX0_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_6533</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ADC45140.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADC45140">
        <property type="protein sequence ID" value="ADC45140"/>
        <property type="gene ID" value="Rmet_6533"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6533"/>
    <dbReference type="HOGENOM" id="CLU_2481052_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADC45140.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="87" mass="8971" checksum="AB54B77A8F00F023" modified="2010-03-23" version="1">MAITEAVGITAEMVDLITIEITITEIVIGAITGITILTGIAGRAARTTSVCPDRVPGGHGWVGGSLRISRRGRSSPAEASAPLRRCT</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-03-23" modified="2020-10-07" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>D3DYH5</accession>
    <name>D3DYH5_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_6767</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ADC45345.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_6767"/>
    <dbReference type="EnsemblBacteria" id="ADC45345">
        <property type="protein sequence ID" value="ADC45345"/>
        <property type="gene ID" value="Rmet_6767"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6767"/>
    <dbReference type="HOGENOM" id="CLU_3347679_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="14"/>
            <end position="36"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADC45345.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="37" mass="3887" checksum="D8CE04BA998AE762" modified="2010-03-23" version="1">MVTKQDDAFILRNLIALAAVETLGGAIALGLVFNLLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>Q1L9H3</accession>
    <name>Q1L9H3_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Mercuric resistance operon regulatory protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">merR</name>
        <name evidence="11" type="ordered locus">Rmet_6344</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="11 12">
        <name>pMOL28</name>
    </geneLocation>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="12">Plasmid pMOL28</plasmid>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Mediates the mercuric-dependent induction of mercury resistance operon. In the absence of mercury MerR represses transcription by binding tightly to the mer operator region; when mercury is present the dimeric complex binds a single ion and becomes a potent transcriptional activator, while remaining bound to the mer site.</text>
    </comment>
    <dbReference type="EMBL" id="CP000355">
        <property type="protein sequence ID" value="ABF13203.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF13203">
        <property type="protein sequence ID" value="ABF13203"/>
        <property type="gene ID" value="Rmet_6344"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6344"/>
    <dbReference type="HOGENOM" id="CLU_060077_2_0_4"/>
    <dbReference type="OMA" id="HRTESSY"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid pMOL28"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045340">
        <property type="term" value="F:mercury ion binding"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046689">
        <property type="term" value="P:response to mercury ion"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04783">
        <property type="entry name" value="HTH_MerR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009061">
        <property type="entry name" value="DNA-bd_dom_put_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011794">
        <property type="entry name" value="MerR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000551">
        <property type="entry name" value="MerR-type_HTH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015358">
        <property type="entry name" value="Tscrpt_reg_MerR_DNA-bd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00376">
        <property type="entry name" value="MerR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09278">
        <property type="entry name" value="MerR-DNA-bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00040">
        <property type="entry name" value="HTHMERR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00422">
        <property type="entry name" value="HTH_MERR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46955">
        <property type="entry name" value="SSF46955"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02051">
        <property type="entry name" value="MerR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00552">
        <property type="entry name" value="HTH_MERR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50937">
        <property type="entry name" value="HTH_MERR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0010">Activator</keyword>
    <keyword evidence="6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0475">Mercuric resistance</keyword>
    <keyword evidence="4" id="KW-0476">Mercury</keyword>
    <keyword evidence="11" id="KW-0614">Plasmid</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0678">Repressor</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH merR-type" evidence="10">
        <location>
            <begin position="25"/>
            <end position="94"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00017146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00024874"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ABF13203.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="162" mass="17734" checksum="8DCEA6CEC5177A82" modified="2006-05-30" version="1">MKLDSVTLLPYSCTESSDMENNLENLTIGVFAKAAGVNVETIRFYQRKGLLLEPDKPYGSIRRYGEADVTRVRFVKSAQRLGFSLDEIAELLRLEDGTHCEEASSLAEHKLKDVREKMADLARMEAVLSELVCACHARRGNVSCPLIASLQGGASLAGSAMP</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="113" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LAJ7</accession>
    <name>Q1LAJ7_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">CzcP cation efflux P1-ATPase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">czcP</name>
        <name evidence="8" type="ordered locus">Rmet_5970</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="8 9">
        <name>pMOL30</name>
    </geneLocation>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="9">Plasmid pMOL30</plasmid>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="journal article" date="2015" name="Nat. Chem. Biol." volume="11" first="678" last="684">
            <title>A new metal binding domain involved in cadmium, cobalt and zinc transport.</title>
            <authorList>
                <person name="Smith A.T."/>
                <person name="Barupala D."/>
                <person name="Stemmler T.L."/>
                <person name="Rosenzweig A.C."/>
            </authorList>
            <dbReference type="PubMed" id="26192600"/>
            <dbReference type="DOI" id="10.1038/nchembio.1863"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (2.17 ANGSTROMS) OF 2-187</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cell membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IB subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP000354">
        <property type="protein sequence ID" value="ABF12829.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011514822.1">
        <property type="nucleotide sequence ID" value="NC_007971.2"/>
    </dbReference>
    <dbReference type="PDB" id="4U9R">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.17 A"/>
        <property type="chains" value="A=2-187"/>
    </dbReference>
    <dbReference type="PDBsum" id="4U9R"/>
    <dbReference type="SMR" id="Q1LAJ7"/>
    <dbReference type="TCDB" id="3.A.3.6.27">
        <property type="family name" value="the p-type atpase (p-atpase) superfamily"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF12829">
        <property type="protein sequence ID" value="ABF12829"/>
        <property type="gene ID" value="Rmet_5970"/>
    </dbReference>
    <dbReference type="GeneID" id="60820436"/>
    <dbReference type="KEGG" id="rme:Rmet_5970"/>
    <dbReference type="PATRIC" id="fig|402626.5.peg.2923"/>
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        <property type="organism ID" value="5272"/>
    </dbReference>
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        <property type="component" value="Plasmid pMOL30"/>
    </dbReference>
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        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
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        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="F:ATPase-coupled cation transmembrane transporter activity"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1110.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
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    </dbReference>
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        <property type="entry name" value="ATPase_P-typ_cyto_dom_N"/>
    </dbReference>
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    </dbReference>
    <dbReference type="InterPro" id="IPR023298">
        <property type="entry name" value="ATPase_P-typ_TM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008250">
        <property type="entry name" value="ATPase_P-typ_transduc_dom_A_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006121">
        <property type="entry name" value="HMA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036163">
        <property type="entry name" value="HMA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027256">
        <property type="entry name" value="P-typ_ATPase_IB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001757">
        <property type="entry name" value="P_typ_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044492">
        <property type="entry name" value="P_typ_ATPase_HD_dom"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00027">
        <property type="entry name" value="p-type_atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55008">
        <property type="entry name" value="SSF55008"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="SSF56784"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81653">
        <property type="entry name" value="SSF81653"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81665">
        <property type="entry name" value="SSF81665"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01525">
        <property type="entry name" value="ATPase-IB_hvy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01494">
        <property type="entry name" value="ATPase_P-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00154">
        <property type="entry name" value="ATPASE_E1_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword evidence="10" id="KW-0002">3D-structure</keyword>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0614">Plasmid</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-1278">Translocase</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="192"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="218"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="436"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="467"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="780"/>
            <end position="798"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="153"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="153"/>
            <end position="180"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU362081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ABF12829.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="10">
        <source>
            <dbReference type="PDB" id="4U9R"/>
        </source>
    </evidence>
    <sequence length="829" mass="86994" checksum="1245FEF339D75978" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LAP2</accession>
    <name>Q1LAP2_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">3-dmu-9_3-mt domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_5925</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12784.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008645464.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5925"/>
    <dbReference type="EnsemblBacteria" id="ABF12784">
        <property type="protein sequence ID" value="ABF12784"/>
        <property type="gene ID" value="Rmet_5925"/>
    </dbReference>
    <dbReference type="GeneID" id="60823722"/>
    <dbReference type="KEGG" id="rme:Rmet_5925"/>
    <dbReference type="eggNOG" id="COG2764">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046006_17_1_4"/>
    <dbReference type="OMA" id="WGDYFGS"/>
    <dbReference type="OrthoDB" id="2051456at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="CDD" id="cd06588">
        <property type="entry name" value="PhnB_like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029068">
        <property type="entry name" value="Glyas_Bleomycin-R_OHBP_Dase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028973">
        <property type="entry name" value="PhnB-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06983">
        <property type="entry name" value="3-dmu-9_3-mt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54593">
        <property type="entry name" value="SSF54593"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="3-dmu-9_3-mt" evidence="1">
        <location>
            <begin position="4"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF06983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF12784.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="142" mass="15282" checksum="3D8AB236208B49CB" modified="2006-05-30" version="1">MSSKVQPYLFFDGRAEEAAEFYKTALGAQVGAMLRYRDGPKDAGPGCGPAVINQDKIMHMALQIGDAVIMGSDGEAKGKTEFKGFGLSLNVANAAECDKTMEKLAEGGQINMPPSQTFFSERFGMVTDKFGVMWMVIAGPKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-10-07" version="53" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LB75</accession>
    <name>Q1LB75_CUPMC</name>
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        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_5742</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12601.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5742"/>
    <dbReference type="EnsemblBacteria" id="ABF12601">
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        <property type="gene ID" value="Rmet_5742"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5742"/>
    <dbReference type="eggNOG" id="COG2128">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2466761_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029032">
        <property type="entry name" value="AhpD-like"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69118">
        <property type="entry name" value="SSF69118"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF12601.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="88" mass="9241" checksum="B713A189469EE2B4" modified="2006-05-30" version="1">MTRVADTHVPDADFNSAAAVFGEKELADLTIAISLMNAYNRLAIAFRPAPDSTLAAGFLADLSVRHEGGGIEDGVMFGESRGMPSPCD</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-10-07" version="58" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LBA5</accession>
    <name>Q1LBA5_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_5712</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12571.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5712"/>
    <dbReference type="EnsemblBacteria" id="ABF12571">
        <property type="protein sequence ID" value="ABF12571"/>
        <property type="gene ID" value="Rmet_5712"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5712"/>
    <dbReference type="eggNOG" id="COG0859">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2651869_0_0_4"/>
    <dbReference type="OrthoDB" id="370520at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF12571.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="76" mass="8455" checksum="70C96B063301843C" modified="2006-05-30" version="1">MCTSVAHLAGALGKPTWVMLDVNASWQWLLGRSDSPWYPTTRLYRQVTYRDWQPVLEQVRADPLELAGGIPAAGAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LBH6</accession>
    <name>Q1LBH6_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Methyl-accepting chemotaxis sensory transducer</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">Rmet_5641</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="11">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="11">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12500.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011520045.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5641"/>
    <dbReference type="EnsemblBacteria" id="ABF12500">
        <property type="protein sequence ID" value="ABF12500"/>
        <property type="gene ID" value="Rmet_5641"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5641"/>
    <dbReference type="eggNOG" id="COG0840">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_107_21_4"/>
    <dbReference type="OMA" id="AYSHYDE"/>
    <dbReference type="OrthoDB" id="429006at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004888">
        <property type="term" value="F:transmembrane signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006935">
        <property type="term" value="P:chemotaxis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004090">
        <property type="entry name" value="Chemotax_Me-accpt_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004089">
        <property type="entry name" value="MCPsignal_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033480">
        <property type="entry name" value="sCache_2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00015">
        <property type="entry name" value="MCPsignal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17200">
        <property type="entry name" value="sCache_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00260">
        <property type="entry name" value="CHEMTRNSDUCR"/>
    </dbReference>
    <dbReference type="SMART" id="SM01049">
        <property type="entry name" value="Cache_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00283">
        <property type="entry name" value="MA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50111">
        <property type="entry name" value="CHEMOTAXIS_TRANSDUC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0614">Plasmid</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="domain" description="Methyl-accepting transducer" evidence="9">
        <location>
            <begin position="271"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="471"/>
            <end position="509"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ABF12500.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="519" mass="55736" checksum="D3F22D2E3C6663CC" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="57" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LBI3</accession>
    <name>Q1LBI3_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_5634</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12493.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011520037.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF12493">
        <property type="protein sequence ID" value="ABF12493"/>
        <property type="gene ID" value="Rmet_5634"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5634"/>
    <dbReference type="HOGENOM" id="CLU_172555_0_0_4"/>
    <dbReference type="OMA" id="DAKIMNA"/>
    <dbReference type="OrthoDB" id="2002079at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF12493.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="111" mass="12778" checksum="A216DC6F7AC4774A" modified="2006-05-30" version="1">MLQASEIQTRFSHLQRTVSETSKTIHADSAIPKDLMSCMDELDKECKSAKKIMSSRNEGRIRQCVDDLERMGDRAERVCHDTDGLHHRVMDAVSAMHLELADLKRDMHEMH</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LBN8</accession>
    <name>Q1LBN8_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Cytochrome b561 family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Rmet_5579</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="5">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="5">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12438.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519988.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5579"/>
    <dbReference type="DNASU" id="4042440"/>
    <dbReference type="EnsemblBacteria" id="ABF12438">
        <property type="protein sequence ID" value="ABF12438"/>
        <property type="gene ID" value="Rmet_5579"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5579"/>
    <dbReference type="eggNOG" id="COG3658">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_078451_2_1_4"/>
    <dbReference type="OMA" id="WGEEWLE"/>
    <dbReference type="OrthoDB" id="923657at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022904">
        <property type="term" value="P:respiratory electron transport chain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011577">
        <property type="entry name" value="Cyt_b561_bac/Ni-Hgenase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016174">
        <property type="entry name" value="Di-haem_cyt_TM"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01292">
        <property type="entry name" value="Ni_hydr_CYTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81342">
        <property type="entry name" value="SSF81342"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0614">Plasmid</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="47"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="103"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="144"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="domain" description="Ni_hydr_CYTB" evidence="3">
        <location>
            <begin position="16"/>
            <end position="176"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01292"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF12438.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="184" mass="20288" checksum="95F586F7DE0ACEF4" modified="2006-05-30" version="1">MSAPADRTNAPDTIRVWDVVVRFTHWSVAAIVCFDLVQDSGDKVHRTLGYIAAGLVLFRVLWGFIGSQPARFRTWLPRPSAVLAYAKALIAGRAPRHVSHTPLGAVAMLAMWTLILALAVTGWISRLDAFWGEDLPVDIHRWLANALLALVILHVAAAIVMSIKGRENLVAAMITGRKPRDPRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LBT7</accession>
    <name>Q1LBT7_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Acyl-CoA dehydrogenase putative medium-chain acyl-CoA dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Rmet_5530</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="9">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="9">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the acyl-CoA dehydrogenase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12389.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519933.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5530"/>
    <dbReference type="EnsemblBacteria" id="ABF12389">
        <property type="protein sequence ID" value="ABF12389"/>
        <property type="gene ID" value="Rmet_5530"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5530"/>
    <dbReference type="eggNOG" id="COG1960">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018204_9_0_4"/>
    <dbReference type="OMA" id="MRWNALR"/>
    <dbReference type="OrthoDB" id="760677at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016627">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-CH group of donors"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.540.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.110.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006091">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046373">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036250">
        <property type="entry name" value="AcylCo_DH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009075">
        <property type="entry name" value="AcylCo_DH/oxidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013786">
        <property type="entry name" value="AcylCoA_DH/ox_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037069">
        <property type="entry name" value="AcylCoA_DH/ox_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009100">
        <property type="entry name" value="AcylCoA_DH/oxidase_NM_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00441">
        <property type="entry name" value="Acyl-CoA_dh_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02770">
        <property type="entry name" value="Acyl-CoA_dh_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02771">
        <property type="entry name" value="Acyl-CoA_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47203">
        <property type="entry name" value="SSF47203"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56645">
        <property type="entry name" value="SSF56645"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0274">FAD</keyword>
    <keyword evidence="3" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="8" id="KW-0614">Plasmid</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Acyl-CoA_dh_N" evidence="7">
        <location>
            <begin position="7"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA_dh_M" evidence="6">
        <location>
            <begin position="125"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA_dh_1" evidence="5">
        <location>
            <begin position="231"/>
            <end position="384"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF02770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF02771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ABF12389.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="386" mass="43533" checksum="ED3F03490F2651FE" modified="2006-05-30" version="1">MDLRLSPAQEAFRAEVRAFIEERLPEDIRARLRAGHPAQKEDTVTWQRILNERGWAAPHWPRKFGGAELGQLERLILLDEMFRAPTPLPQVFNVTMLGPVLMKFGTPEQCEYFLPKLANLDLWFCQGFSEPGSGSDLASLRTAAKRDGDHYVVNGQKIWTTTAHWADWVFALVRTNPEAKKQEGISFLLIDLKSPGITIRPIRSIDGDHHLNEVFFDDVRVPVENLVGEENKGWNCAKFLLGNERTGIANVGLCRERLDYARELADRTVQGENRLIDDPKLRSEIATLDAEVRALEVTNWRFLLTPNEQARIPAFASVLKLKGVELQQDINALLARLAGPQGLEKRGKRDTDRGPLTPRYFYSRAASIYGGTSEVQKDILAKAIVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="92" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LC35</accession>
    <name>Q1LC35_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Acetyl-CoA acetyltransferase</fullName>
            <ecNumber evidence="6">2.3.1.16</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Rmet_5432</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="7">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="7">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the thiolase-like superfamily. Thiolase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.16" evidence="6"/>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12291.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519839.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5432"/>
    <dbReference type="EnsemblBacteria" id="ABF12291">
        <property type="protein sequence ID" value="ABF12291"/>
        <property type="gene ID" value="Rmet_5432"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5432"/>
    <dbReference type="eggNOG" id="COG0183">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031026_2_3_4"/>
    <dbReference type="OMA" id="YAYESHQ"/>
    <dbReference type="OrthoDB" id="550338at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003988">
        <property type="term" value="F:acetyl-CoA C-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd00751">
        <property type="entry name" value="thiolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.47.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002155">
        <property type="entry name" value="Thiolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016039">
        <property type="entry name" value="Thiolase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020617">
        <property type="entry name" value="Thiolase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020613">
        <property type="entry name" value="Thiolase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020616">
        <property type="entry name" value="Thiolase_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02803">
        <property type="entry name" value="Thiolase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00108">
        <property type="entry name" value="Thiolase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000429">
        <property type="entry name" value="Ac-CoA_Ac_transf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53901">
        <property type="entry name" value="SSF53901"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01930">
        <property type="entry name" value="AcCoA-C-Actrans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00737">
        <property type="entry name" value="THIOLASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="6" id="KW-0614">Plasmid</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Thiolase_N" evidence="4">
        <location>
            <begin position="5"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="domain" description="Thiolase_C" evidence="5">
        <location>
            <begin position="270"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="active site" description="Acyl-thioester intermediate" evidence="2">
        <location>
            <position position="89"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="2">
        <location>
            <position position="348"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="2">
        <location>
            <position position="378"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR000429-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF02803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABF12291.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="392" mass="41218" checksum="7FABF4C7ED504CD9" modified="2006-05-30" version="1">MSEAYIVSAVRTAGGRRNGRLSGWHPADLAGQVLDALVERSGADPSMVEDVIMGCVGQAGEQSSNIARGAVLASKLPESVPATSVDRQCGSSQQALHFAAQAVMSGTMDVVIAAGVESMSRVPMGTPSTLPRKNGLGHYVSPQMQARYPGVEFSQFTGAEMIAKKYKLDKTALDAYALRSHQLAAAATKAGRFEQEILPLAARMNDGSDLGEFHTIDEGIRFDATLEGIASVKLIAEGGSVTAATASQICDGASGVMVVNERGLKMLGVKPLARIHHMSVLGHDPVVMLEAPLPATERALKRAGMRIDDIDLYEVNEAFAPVPLAWLQALGADADRLNVNGGAIALGHPLGGSGTKLMTTLIHALHQRGKRYGLQTMCEGGGMANVTIVERL</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LCE7</accession>
    <name>Q1LCE7_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Membrane fusion protein heavy metal cation tricomponent efflux ZniB(CzcB-like)(RND-HME-MFP)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">zniB</name>
        <name evidence="4" type="ordered locus">Rmet_5320</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="5">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="5">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12179.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519723.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5320"/>
    <dbReference type="EnsemblBacteria" id="ABF12179">
        <property type="protein sequence ID" value="ABF12179"/>
        <property type="gene ID" value="Rmet_5320"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5320"/>
    <dbReference type="eggNOG" id="COG0845">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018816_13_3_4"/>
    <dbReference type="OMA" id="MIDVPPE"/>
    <dbReference type="OrthoDB" id="1174564at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032317">
        <property type="entry name" value="HlyD_D23"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006143">
        <property type="entry name" value="RND_pump_MFP"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16576">
        <property type="entry name" value="HlyD_D23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01730">
        <property type="entry name" value="RND_mfp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0614">Plasmid</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="7"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="domain" description="HlyD_D23" evidence="3">
        <location>
            <begin position="80"/>
            <end position="320"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF16576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF12179.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="404" mass="42792" checksum="059FF1A2CAE2EE14" modified="2006-05-30" version="1">MRFDAKSAILGGGATAVVLVGLMFAFNAWTQPSPLYGPEKGFGQGGPSAPKVAPRGSSVTLTQTQMGTVKVVSVQDYAFANQREAVGNIDFNQDRSVQVFTAYQGKVRNVFVKVGEDVAKGAPLFDIESPDLVQAESTLISAAGARKLNAKALERAHQLFEIQGIAQKDLDQAISDQQAAEAAYKAARDAVAIFGKSASEMDRIIEQRRTDPVLTVFSPIAGRVTARNAQPGLLVQPGNSPAPITVSDISTVWMQAFVTEADSPLLKVGQAVKVHAMAFPGREFAGTITTTGASIDSATHRLMVRSEVKDPKHELRPGMITSFSIRTGEPDHSAAVAADGLVREGDGTMTVWTTTDQRRFERRVVTVGQTQDGLVQILSGLAPGELVASEGALFLSNAAALAAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LCG7</accession>
    <name>Q1LCG7_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Flagellar protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">fliO</name>
        <name evidence="8" type="ordered locus">Rmet_5300</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="9">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="9">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Bacterial flagellum basal body</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the FliO/MopB family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12159.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519705.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5300"/>
    <dbReference type="EnsemblBacteria" id="ABF12159">
        <property type="protein sequence ID" value="ABF12159"/>
        <property type="gene ID" value="Rmet_5300"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5300"/>
    <dbReference type="eggNOG" id="COG3190">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_113213_0_0_4"/>
    <dbReference type="OMA" id="MNAVNNA"/>
    <dbReference type="OrthoDB" id="1800754at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009425">
        <property type="term" value="C:bacterial-type flagellum basal body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044781">
        <property type="term" value="P:bacterial-type flagellum organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022781">
        <property type="entry name" value="Flagellar_biosynth_FliO"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04347">
        <property type="entry name" value="FliO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03500">
        <property type="entry name" value="FliO_TIGR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0975">Bacterial flagellum</keyword>
    <keyword evidence="1 5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-0966">Cell projection</keyword>
    <keyword evidence="8" id="KW-0969">Cilium</keyword>
    <keyword evidence="8" id="KW-0282">Flagellum</keyword>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0614">Plasmid</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193305" description="Flagellar protein" evidence="7">
        <location>
            <begin position="25"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="67"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="143"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="147"/>
            <end position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU362064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ABF12159.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="186" mass="18716" checksum="B2F23375B06E3B40" modified="2006-05-30" version="1">MMVAARRLAAGIALAGVAASGAQAADVVLSTAQAGMPTGGAATNTTTSATTSNTGANAPVISSGASLAQAGLGLFAVIALILAMAWVARRVGLVRQGSGNASMKVVGSLMLGPRQRVVTVEVGGTWLVLGVSPNEIRPLHTLEAQPHTGSPDDSRPGSQADSGVSRNMQGGFGERLMRAMQENLKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LCP0</accession>
    <name>Q1LCP0_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Hydrolase_4 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_5227</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12086.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519632.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5227"/>
    <dbReference type="ESTHER" id="ralme-q1lcp0">
        <property type="family name" value="Xaa-Pro-like_dom"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF12086">
        <property type="protein sequence ID" value="ABF12086"/>
        <property type="gene ID" value="Rmet_5227"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5227"/>
    <dbReference type="eggNOG" id="COG1073">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_048587_1_1_4"/>
    <dbReference type="OMA" id="CPILFCV"/>
    <dbReference type="OrthoDB" id="721431at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022742">
        <property type="entry name" value="Hydrolase_4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12146">
        <property type="entry name" value="Hydrolase_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="SSF53474"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Hydrolase_4" evidence="1">
        <location>
            <begin position="30"/>
            <end position="148"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF12086.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="315" mass="34430" checksum="A7A513B7421AE46C" modified="2006-05-30" version="1">MVSIDSSFQSHGERCAATLMLPSQGTRPPVIIMAHGFGAIRAAGLSAFAERFVAEGYAAYLFDYRNFGDSEGKPRHWVSPRRHLQDWAAAIAHMSSLPEVDADRMVLWGTSFSGGHVIQTAAADHRVRAVIAQVPHVSGLASMKQVPFHLLLRMMLAAMRDLCGSVIRREHYSPIIGRHGDMAALTGDDAWHGYARLLPAGTAWENKVLSRIFLEVPLYSPIRHAHKVAAPTLIVAGTHDTITPASAARRASRRIPYCEFHLVEGNHFELHLHDEPAFASSIAAQLAFLNRHVGVAPIALEKSGRDHRAGSGSRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LCV0</accession>
    <name>Q1LCV0_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">High-potential iron-sulfur protein</fullName>
            <shortName evidence="2">HiPIP</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">hip</name>
        <name evidence="4" type="ordered locus">Rmet_5167</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="5">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="5">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Specific class of high-redox-potential 4Fe-4S ferredoxins. Functions in anaerobic electron transport in most purple and in some other photosynthetic bacteria and in at least one genus (Paracoccus) of halophilic, denitrifying bacteria.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the high-potential iron-sulfur protein (HiPIP) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12026.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5167"/>
    <dbReference type="EnsemblBacteria" id="ABF12026">
        <property type="protein sequence ID" value="ABF12026"/>
        <property type="gene ID" value="Rmet_5167"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5167"/>
    <dbReference type="eggNOG" id="ENOG50330XW">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_147871_0_0_4"/>
    <dbReference type="OMA" id="VNAKGWC"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019646">
        <property type="term" value="P:aerobic electron transport chain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.490.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000170">
        <property type="entry name" value="High_potential_FeS_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036369">
        <property type="entry name" value="HIPIP_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01355">
        <property type="entry name" value="HIPIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57652">
        <property type="entry name" value="SSF57652"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51373">
        <property type="entry name" value="HIPIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="2" id="KW-0249">Electron transport</keyword>
    <keyword evidence="2" id="KW-0408">Iron</keyword>
    <keyword evidence="2" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="1 2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0614">Plasmid</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="HIPIP" evidence="3">
        <location>
            <begin position="58"/>
            <end position="135"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU000620"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51373"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF12026.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="135" mass="14538" checksum="0AB8026BCD0414EB" modified="2006-05-30" version="1">MKIHSATRSRCRRLDALAHPCEFQPGGGIAMTNRRRFLSSISGVVLATVAGVDGKAEAQTTAKLDEKDPQAVALGYKHDTSKVDKAKFAKHDVSQKCSNCQLYQGKPGDAWGPCPIFAGKQVSANGWCNSYVKKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LCW8</accession>
    <name>Q1LCW8_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Extra-cytoplasmic solute receptor (Bug)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">bugT</name>
        <name evidence="3" type="ordered locus">Rmet_5149</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Periplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the UPF0065 (bug) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12008.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519555.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5149"/>
    <dbReference type="EnsemblBacteria" id="ABF12008">
        <property type="protein sequence ID" value="ABF12008"/>
        <property type="gene ID" value="Rmet_5149"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5149"/>
    <dbReference type="eggNOG" id="COG3181">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045683_0_0_4"/>
    <dbReference type="OMA" id="ATNVAMF"/>
    <dbReference type="OrthoDB" id="447734at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042597">
        <property type="term" value="C:periplasmic space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005064">
        <property type="entry name" value="BUG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042100">
        <property type="entry name" value="Bug_dom1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42928">
        <property type="entry name" value="PTHR42928"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03401">
        <property type="entry name" value="TctC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017082">
        <property type="entry name" value="YflP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-0675">Receptor</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF12008.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="328" mass="35222" checksum="E49527D6F5C7E182" modified="2006-05-30" version="1">MKLTSATRRWAMLCALVVTIGGALTPMDARSEYPDKPIKLVVSFPPGSGTDSNARYLARKMEERLGKPVVVENRPGGNSFIAAQTVTRAEPDGYTLLLASNSPVATNVAMFRKLPYDPVSELAPIARLGVGAMALVVSATSRYQTVDALLSAAREHPGTLNYGAGSASYQIATELFLAMGHIKANHVPYKGAAPALTDLAAGQVDFVFADYGAVLPLLNGGKLRLLAVTSDKRLGSQPNTPTLQESGFPGYFMVNWTAAFAPANTPKPVIAKLEMLMLDIYRTPETSEFLSRTSWDVFVGDAKALRTFQLAEIKKWSDAAERAGVPKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="95" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LD11</accession>
    <name>Q1LD11_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Transcriptional regulator, LysR family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_5106</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the LysR transcriptional regulatory family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11965.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519512.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5106"/>
    <dbReference type="EnsemblBacteria" id="ABF11965">
        <property type="protein sequence ID" value="ABF11965"/>
        <property type="gene ID" value="Rmet_5106"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5106"/>
    <dbReference type="eggNOG" id="COG0583">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039613_6_0_4"/>
    <dbReference type="OMA" id="HVRLLCN"/>
    <dbReference type="OrthoDB" id="990666at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005119">
        <property type="entry name" value="LysR_subst-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000847">
        <property type="entry name" value="Tscrpt_reg_HTH_LysR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00126">
        <property type="entry name" value="HTH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03466">
        <property type="entry name" value="LysR_substrate"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50931">
        <property type="entry name" value="HTH_LYSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH lysR-type" evidence="2">
        <location>
            <begin position="1"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF11965.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="315" mass="33300" checksum="2BE8528FFB7D81A9" modified="2006-05-30" version="1">MRYELTDLRLFQAIAEAHSLAGGASAAHITASAASYRLKNLEHALGTPLFVRNARGMDLTPAGEMMLAHVRELLQGIERMHGEVGRFASGLKGHIRLLANSSSLNGFIIPSVSRFLAAHPAVNIDLEERASQAITAAVAAHEADIGIFASDMTMAADTAGVHAVRYAVDELVLAVAPDHPLARQGRIRFGAALGFDFVCMSRTSSNFLFLRDTAQRAGKQPNVRLHAHSFEAVLALAAAGVGVALVPRSVAAASMLEGRVVGVALDEPWAVRELTLVTRADGKLPGFAAAFVQFLLNDPRVAITRETGAAPDAAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LD23</accession>
    <name>Q1LD23_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Enoyl-CoA hydratase</fullName>
            <ecNumber evidence="4">4.2.1.17</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Rmet_5094</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="5">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="5">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the enoyl-CoA hydratase/isomerase family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.17" evidence="4"/>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11953.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519499.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5094"/>
    <dbReference type="EnsemblBacteria" id="ABF11953">
        <property type="protein sequence ID" value="ABF11953"/>
        <property type="gene ID" value="Rmet_5094"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5094"/>
    <dbReference type="eggNOG" id="COG1024">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009834_7_2_4"/>
    <dbReference type="OMA" id="RPPRFTW"/>
    <dbReference type="OrthoDB" id="1275789at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004300">
        <property type="term" value="F:enoyl-CoA hydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.12.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029045">
        <property type="entry name" value="ClpP/crotonase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001753">
        <property type="entry name" value="Enoyl-CoA_hydra/iso"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014748">
        <property type="entry name" value="Enoyl-CoA_hydra_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00378">
        <property type="entry name" value="ECH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52096">
        <property type="entry name" value="SSF52096"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0456">Lyase</keyword>
    <keyword evidence="4" id="KW-0614">Plasmid</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="266"/>
            <end position="289"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF11953.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="289" mass="31213" checksum="F93AFE668B97A5DB" modified="2006-05-30" version="1">MTTTPPAFETLQYAVQNGIATISLHRPEKMNAFTHQMCEDLIAAFDATDADDNVRAVIVTGSGRAFCAGADLSSGGATFDYEKRYGSTGVKRDGGGRVVLRIFRSLKPVIAAVNGAAVGVGVTMQLPMDIRIASTDAKFGLVFARRGITPEAASSWFLSRVVGISTALEWCFSGRVFSAQEAHERGLVRSLHAPEDLLPAAYAIAREIADNAAPVSVAMARQMIWRMAGAAHPMDAHKVDSRAIQSRGQSDDAKEGIASFLEKRPAQFPNRVSSQMPDFFDWQGEPDFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LD87</accession>
    <name>Q1LD87_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative acyl dehydratase, MaoC-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_5027</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11889.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519438.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5027"/>
    <dbReference type="EnsemblBacteria" id="ABF11889">
        <property type="protein sequence ID" value="ABF11889"/>
        <property type="gene ID" value="Rmet_5027"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5027"/>
    <dbReference type="eggNOG" id="COG2030">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_094876_4_1_4"/>
    <dbReference type="OMA" id="AEIVFNG"/>
    <dbReference type="OrthoDB" id="1924578at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029069">
        <property type="entry name" value="HotDog_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002539">
        <property type="entry name" value="MaoC-like_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01575">
        <property type="entry name" value="MaoC_dehydratas"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54637">
        <property type="entry name" value="SSF54637"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MaoC-like" evidence="1">
        <location>
            <begin position="22"/>
            <end position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01575"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF11889.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="138" mass="15459" checksum="E771E22219B03C9A" modified="2006-05-30" version="1">MSMPNFEQIQVGDTLPPLTLEPINRTTLALFAGASNDHNAIHIDIDYARKAGMPDVFAHGMLSMAYLGRLLTLWVDQRQIRDFGVRFVGITHLGHRITCTGHVVEKFEADGERRVKVELRTANQYDETRILGDAVVAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LDE0</accession>
    <name>Q1LDE0_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Transposon related protein TniQ (Tn6048)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">tniQ</name>
        <name evidence="1" type="ordered locus">Rmet_3008</name>
        <name evidence="2" type="ordered locus">Rmet_4043</name>
        <name evidence="3" type="ordered locus">Rmet_4974</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid</name>
    </geneLocation>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="submission" date="2006-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of the chromosome of Ralstonia metallidurans CH34.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Copeland A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Barry K."/>
                <person name="Detter J.C."/>
                <person name="Glavina del Rio T."/>
                <person name="Hammon N."/>
                <person name="Israni S."/>
                <person name="Dalin E."/>
                <person name="Tice H."/>
                <person name="Martinez M."/>
                <person name="Goltsman E."/>
                <person name="Pitluck S."/>
                <person name="Schmutz J."/>
                <person name="Larimer F."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Kim E."/>
                <person name="Mergeay M."/>
                <person name="Benotmane M.A."/>
                <person name="Vallaeys T."/>
                <person name="Michaux A."/>
                <person name="Monchy S."/>
                <person name="Dunn J."/>
                <person name="McCorkle S."/>
                <person name="Taghavi S."/>
                <person name="van der Lelie D."/>
                <person name="Richardson P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">CH34</strain>
            <plasmid evidence="3">megaplasmid</plasmid>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2010-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Benotmane R."/>
                <person name="Lapidus A."/>
                <person name="McCorkle S."/>
                <person name="Monchy S."/>
                <person name="Taghavi S."/>
                <person name="van der Lelie N."/>
                <person name="Dunn J."/>
                <person name="Leys N."/>
                <person name="Mergeay M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">CH34</strain>
            <plasmid evidence="3">megaplasmid</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09881.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10911.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11836.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517528.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3008"/>
    <dbReference type="EnsemblBacteria" id="ABF09881">
        <property type="protein sequence ID" value="ABF09881"/>
        <property type="gene ID" value="Rmet_3008"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF10911">
        <property type="protein sequence ID" value="ABF10911"/>
        <property type="gene ID" value="Rmet_4043"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF11836">
        <property type="protein sequence ID" value="ABF11836"/>
        <property type="gene ID" value="Rmet_4974"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3008"/>
    <dbReference type="KEGG" id="rme:Rmet_4043"/>
    <dbReference type="KEGG" id="rme:Rmet_4974"/>
    <dbReference type="eggNOG" id="ENOG502Z8WC">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_056355_0_0_4"/>
    <dbReference type="OMA" id="TCAGYLR"/>
    <dbReference type="OrthoDB" id="935321at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009492">
        <property type="entry name" value="TniQ"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06527">
        <property type="entry name" value="TniQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF09881.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF10911.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF11836.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="405" mass="45501" checksum="0186C0C33DED3067" modified="2006-05-30" version="1">MKPAPRWPLHPAPREGEALSSWLNRVALCYHMEVSELLEHDLGHAQVDDLDTAPPLALLAMLSQRSGIEPDRLRCMSFAGWVPWLLDSLDDQIPDALETYAFQLSVLLPKLRRRTRSITSWRAWLPSQPIHRACPLCLNDPANQAVLLAWKLPLMLSCPLHGCWLESYWGVPGRFLGWENADTASRTASDAIAVMDRRTWQALTTGHVELPCRRIHAGLWFRLLRTLLDELNTPLSACGTYAGYLRQVWQGCGHPLRAGQSLWRPYETLNPAIRLQMLEAAATAISLIEVRDISPPGEHAKLFWSELQTGFTSGLPAKPPKPEPVNHWQRAVQAISEAIIEARHDPETARSLFALASYGRRDPASLEQLRATFAKEGIPPEFLSHYEADGPFACLRQNDGLSDKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LDJ8</accession>
    <name>Q1LDJ8_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Putative transmembrane regulatory protein nosR</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">nosR</name>
        <name evidence="7" type="ordered locus">Rmet_4916</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="8">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="8">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11778.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519337.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4916"/>
    <dbReference type="EnsemblBacteria" id="ABF11778">
        <property type="protein sequence ID" value="ABF11778"/>
        <property type="gene ID" value="Rmet_4916"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4916"/>
    <dbReference type="eggNOG" id="COG0348">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG3901">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013077_0_0_4"/>
    <dbReference type="OMA" id="RLWPIKY"/>
    <dbReference type="OrthoDB" id="1230380at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007329">
        <property type="entry name" value="FMN-bd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12801">
        <property type="entry name" value="Fer4_5"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04205">
        <property type="entry name" value="FMN_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00900">
        <property type="entry name" value="FMN_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0408">Iron</keyword>
    <keyword evidence="3" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0614">Plasmid</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193091" evidence="5">
        <location>
            <begin position="29"/>
            <end position="872"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="414"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="454"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="490"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="558"/>
            <end position="578"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="598"/>
            <end position="616"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="776"/>
            <end position="797"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="803"/>
            <end position="821"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="842"/>
            <end position="864"/>
        </location>
    </feature>
    <feature type="domain" description="FMN_bind" evidence="6">
        <location>
            <begin position="101"/>
            <end position="195"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABF11778.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="872" mass="97309" checksum="C24493A11AD7A958" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-10-07" version="56" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LDT2</accession>
    <name>Q1LDT2_CUPMC</name>
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        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_4832</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11694.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4832"/>
    <dbReference type="EnsemblBacteria" id="ABF11694">
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        <property type="gene ID" value="Rmet_4832"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4832"/>
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        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_086263_1_1_4"/>
    <dbReference type="OMA" id="YLETPTW"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011727">
        <property type="entry name" value="CHP02117"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09601">
        <property type="entry name" value="DUF2459"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF11694.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="225" mass="24132" checksum="5E35FBFE624B520D" modified="2006-05-30" version="1">MWAGWPEPMKRAGLARPCARVAIAALITLLSGCAASTETPAANDPATTIDVVERDWHTDVCIRTEDADTRLMRLVIGYGDSRYLCFGFGDRHYLLSRERGPMTLLSALLPGAGAILLTVLRDTPAAAFGADNVVRLDVSEPGMERLREFLGNAVQTNDTGAPISLGEGPYPGGLYFGATADYDGFYTCNTWTADALRAAGIPILGPVLFADGVMRQVRQIAQPAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="92" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LE02</accession>
    <name>Q1LE02_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">PPK2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Rmet_4762</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="6">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="6">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the polyphosphate kinase 2 (PPK2) family. Class I subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11624.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519195.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4762"/>
    <dbReference type="EnsemblBacteria" id="ABF11624">
        <property type="protein sequence ID" value="ABF11624"/>
        <property type="gene ID" value="Rmet_4762"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4762"/>
    <dbReference type="eggNOG" id="COG2326">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_048699_0_1_4"/>
    <dbReference type="OMA" id="TKLWFSV"/>
    <dbReference type="OrthoDB" id="953793at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008976">
        <property type="term" value="F:polyphosphate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006793">
        <property type="term" value="P:phosphorus metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022488">
        <property type="entry name" value="PPK2-related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022486">
        <property type="entry name" value="PPK2_PA0141"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03976">
        <property type="entry name" value="PPK2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03707">
        <property type="entry name" value="PPK2_P_aer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0614">Plasmid</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PPK2" evidence="4">
        <location>
            <begin position="141"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="41"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009924"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF03976"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABF11624.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="383" mass="43399" checksum="0400656CFA764D0C" modified="2006-05-30" version="1">MAIGKKNTAASEGLQSIDAAQPLAGSSAEERPVNKGDVLPTNSARSAELHAVRDGAEAARVLVASGLRDVLADVAGKDPDTVLATVRTVMDGLSPDEAAELRRLILEGEPSTWPAGKKRHPDEELSPTWRQGTYPYQNLMSRRNYEKQKYLLQVELLKLQAWVRETGQRVVIVFEGRDAAGKGGAIKRFMEHLNPRGARVVALEKPTDTERSQWYFQRYIQHLPAAGEIVLFDRSWYNRAGVEHVMGFCSNAEYHDFLYQAPEFERHIVRSGIHLFKFWFSVSQKEQRRRFKERQVHPLKQWKLSPVDLASLDKWEDYTQAKEAMFARTDTADAPWTVIRSDCKKRARLNAMRVVLHRFAYTNRSPEHVGLVDPLVVGRALAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LE32</accession>
    <name>Q1LE32_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Transcriptional regulator, IclR family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Rmet_4732</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="6">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="6">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11594.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519168.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4732"/>
    <dbReference type="EnsemblBacteria" id="ABF11594">
        <property type="protein sequence ID" value="ABF11594"/>
        <property type="gene ID" value="Rmet_4732"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4732"/>
    <dbReference type="eggNOG" id="COG1414">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_062618_0_0_4"/>
    <dbReference type="OMA" id="EDRHFVT"/>
    <dbReference type="OrthoDB" id="370329at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029016">
        <property type="entry name" value="GAF-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014757">
        <property type="entry name" value="Tscrpt_reg_IclR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005471">
        <property type="entry name" value="Tscrpt_reg_IclR_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09339">
        <property type="entry name" value="HTH_IclR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01614">
        <property type="entry name" value="IclR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00346">
        <property type="entry name" value="HTH_ICLR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51077">
        <property type="entry name" value="HTH_ICLR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51078">
        <property type="entry name" value="ICLR_ED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0614">Plasmid</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH iclR-type" evidence="3">
        <location>
            <begin position="26"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="domain" description="IclR-ED" evidence="4">
        <location>
            <begin position="89"/>
            <end position="272"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABF11594.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="274" mass="29739" checksum="44F0AE0B39335D00" modified="2006-05-30" version="1">MEDVIEMAEIAAAPKESKRKEDRHFVTALARGLDVLACFRAFDSELGNAELAKRCGLPKSTISRLTHTLTELGYLQALTDPVRYRLGSSALALAPAAQQGAEVLAVARPYMQELADLSQGVASLIVRDRGRMLVFENCQSETYLTLRVAVGSRVSGLNTAAGRAYLVALPPAEQRQALTAFRTDEKVAPAEAEAILANSRDEQRRLGCTTSFGEWLPDINSIAHAFRPGPSLPPMTLSCSGPNAIVPPAHLLEKVRPLLRDHIRKIEAAFGVTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LEK3</accession>
    <name>Q1LEK3_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Transcriptional regulator, AsnC family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Rmet_4561</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="6">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="6">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11423.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008649534.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4561"/>
    <dbReference type="EnsemblBacteria" id="ABF11423">
        <property type="protein sequence ID" value="ABF11423"/>
        <property type="gene ID" value="Rmet_4561"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4561"/>
    <dbReference type="eggNOG" id="COG1522">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_091233_0_0_4"/>
    <dbReference type="OMA" id="YEEFLRH"/>
    <dbReference type="OrthoDB" id="1534467at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="CDD" id="cd00090">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011991">
        <property type="entry name" value="ArsR-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000485">
        <property type="entry name" value="AsnC-type_HTH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011008">
        <property type="entry name" value="Dimeric_a/b-barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019888">
        <property type="entry name" value="Tscrpt_reg_AsnC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019887">
        <property type="entry name" value="Tscrpt_reg_AsnC/Lrp_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01037">
        <property type="entry name" value="AsnC_trans_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00033">
        <property type="entry name" value="HTHASNC"/>
    </dbReference>
    <dbReference type="SMART" id="SM00344">
        <property type="entry name" value="HTH_ASNC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54909">
        <property type="entry name" value="SSF54909"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50956">
        <property type="entry name" value="HTH_ASNC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0614">Plasmid</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH asnC-type" evidence="4">
        <location>
            <begin position="7"/>
            <end position="68"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50956"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABF11423.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="156" mass="17689" checksum="36230F70A624BB62" modified="2006-05-30" version="1">MNNPLKLDEIDRRILRELRRDGRISNAKLAERVGLSATPCWNRVKALEDAGVISGYAALLNQKALGLPDTVLIEVTLDRHDDEMFNRFGEALAQLPEVLEAHLLTGEYDYLIKVAVAGTEGYEEFLRHKLYKLPGLRHSHSTFVLRTLKREPSVEP</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LEP2</accession>
    <name>Q1LEP2_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Exonuclease/RNA metabolizing enzyme, metallo-beta-lactamase family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_4519</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11384.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518977.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4519"/>
    <dbReference type="EnsemblBacteria" id="ABF11384">
        <property type="protein sequence ID" value="ABF11384"/>
        <property type="gene ID" value="Rmet_4519"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4519"/>
    <dbReference type="eggNOG" id="COG1236">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009673_5_2_4"/>
    <dbReference type="OMA" id="FSAHGDY"/>
    <dbReference type="OrthoDB" id="580619at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004527">
        <property type="term" value="F:exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022712">
        <property type="entry name" value="Beta_Casp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001279">
        <property type="entry name" value="Metallo-B-lactamas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011108">
        <property type="entry name" value="RMMBL"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10996">
        <property type="entry name" value="Beta-Casp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00753">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07521">
        <property type="entry name" value="RMMBL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01027">
        <property type="entry name" value="Beta-Casp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00849">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="SSF56281"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0540">Nuclease</keyword>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Lactamase_B" evidence="1">
        <location>
            <begin position="13"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="domain" description="Beta-Casp" evidence="2">
        <location>
            <begin position="247"/>
            <end position="366"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM01027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF11384.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="452" mass="50024" checksum="16C5683A385A3A6A" modified="2006-05-30" version="1">MHIQFLGATDTVTGSRFLIDTGEARVLIDCGLFQGYKALRLRNWDRFPISPESLDAVVLTHAHIDHSGYLPLLVKRGYKGRVHCTTGTAELCGLLLPDSAHLAEEDANYANRKGYSRHQPALPLYTVADAMHALEHLKPAPYSKRVTIARGVEAEFHRAGHILGSSSVTIHAKGRRILFSGDLGPQDDMLMRPPEPPTAADYLIVESTYGDRLHPEGDPIELLADIIRKTVARGGSVIIPVFAVGRAQLLLYCLYRLRQRRRFPDVPIYLNSPMAIDATRILTEHTDELRIDPETCAAACSVAIPTRSMEESIRINRVDQPKIILAGSGMLTGGRVVHHLESFGQDPRSTFLLVGFQAAGTRGATLASGQRTLRMHGREVELRASVEQIRHFSAHADADGLMDWMSAIGTPPRQTFIVHGEPGAADALRQRIEHSLHWDVTMPEYRGIYPLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LEQ4</accession>
    <name>Q1LEQ4_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Hippurate hydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">hipO</name>
        <name evidence="3" type="ordered locus">Rmet_4507</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11372.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518965.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4507"/>
    <dbReference type="EnsemblBacteria" id="ABF11372">
        <property type="protein sequence ID" value="ABF11372"/>
        <property type="gene ID" value="Rmet_4507"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4507"/>
    <dbReference type="eggNOG" id="COG1473">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023257_0_1_4"/>
    <dbReference type="OMA" id="ECSCANV"/>
    <dbReference type="OrthoDB" id="796259at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017439">
        <property type="entry name" value="Amidohydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036264">
        <property type="entry name" value="Bact_exopeptidase_dim_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002933">
        <property type="entry name" value="Peptidase_M20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011650">
        <property type="entry name" value="Peptidase_M20_dimer"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11014">
        <property type="entry name" value="PTHR11014"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07687">
        <property type="entry name" value="M20_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01546">
        <property type="entry name" value="Peptidase_M20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005962">
        <property type="entry name" value="Pept_M20D_amidohydro"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55031">
        <property type="entry name" value="SSF55031"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01891">
        <property type="entry name" value="amidohydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="M20_dimer" evidence="2">
        <location>
            <begin position="200"/>
            <end position="290"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF11372.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="403" mass="42872" checksum="D943B402A0674A99" modified="2006-05-30" version="1">MTKAPTLQPFQLLPHLLPPVDIDAETFVNIRRQIHAQPELGFEVGATSTLVTHLLQSWGYDVHTGIGKSGVVGQLKLGNGARRLGIRADMDALPIVEATGLPYASRNHGKMHACGHDGHTAILLAAAKALADSRSFDGTLNLIFQPDEENLCGARAMIEDGLFERFPCDAVFALHNMPGVPAGSFRVLPGPVSLSSDVADITIKGVGGHGAMPHRARDPIAASAAIVTALQTVVARNVAPDDTAVVSIGFIRGGATHNVIPESVTLGVNVRAARAETRAMVEQRIREIVSLTAQAHGVEAHIDYRQLTPPMVNTEAETRLAQRICTDLVGTENVVTQAPKGLNGSEDFAWMLNEVPGCYLILGNGEGEFGGCMVHNPGYDFNDQVLPLGAACWVRLAQAYLTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="107" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LER4</accession>
    <name>Q1LER4_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Ferric siderophore receptor protein (TonB-dependent siderophore receptor)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">ffcA1</name>
        <name evidence="10" type="ordered locus">Rmet_4497</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="11">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="11">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cell outer membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the TonB-dependent receptor family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11362.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4497"/>
    <dbReference type="EnsemblBacteria" id="ABF11362">
        <property type="protein sequence ID" value="ABF11362"/>
        <property type="gene ID" value="Rmet_4497"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4497"/>
    <dbReference type="eggNOG" id="COG4774">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008287_22_0_4"/>
    <dbReference type="OMA" id="AEWDLPW"/>
    <dbReference type="OrthoDB" id="196825at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009279">
        <property type="term" value="C:cell outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015344">
        <property type="term" value="F:siderophore uptake transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038023">
        <property type="term" value="F:signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.130.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.170.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030149">
        <property type="entry name" value="FcuA/FatA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012910">
        <property type="entry name" value="Plug_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037066">
        <property type="entry name" value="Plug_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039423">
        <property type="entry name" value="TonB-dep_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000531">
        <property type="entry name" value="TonB-dep_rcpt_b-brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036942">
        <property type="entry name" value="TonB_rcpt_b-brl_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010105">
        <property type="entry name" value="TonB_sidphr_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32552">
        <property type="entry name" value="PTHR32552"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32552:SF82">
        <property type="entry name" value="PTHR32552:SF82"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07715">
        <property type="entry name" value="Plug"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00593">
        <property type="entry name" value="TonB_dep_Rec"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01783">
        <property type="entry name" value="TonB-siderophor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0998">Cell outer membrane</keyword>
    <keyword evidence="3 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4 10" id="KW-0675">Receptor</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0798">TonB box</keyword>
    <feature type="domain" description="Plug" evidence="9">
        <location>
            <begin position="103"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="domain" description="TonB_dep_Rec" evidence="8">
        <location>
            <begin position="320"/>
            <end position="742"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009810"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003357"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00593"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF07715"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ABF11362.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="744" mass="79705" checksum="2A9D75F9D0E1065C" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-12-02" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LF18</accession>
    <name>Q1LF18_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Holo-(Acyl carrier protein) synthase 2</fullName>
            <ecNumber evidence="3">2.7.8.7</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">acpT</name>
        <name evidence="3" type="ordered locus">Rmet_4393</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EC" id="2.7.8.7" evidence="3"/>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11258.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4393"/>
    <dbReference type="EnsemblBacteria" id="ABF11258">
        <property type="protein sequence ID" value="ABF11258"/>
        <property type="gene ID" value="Rmet_4393"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4393"/>
    <dbReference type="eggNOG" id="COG2091">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1204020_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008897">
        <property type="term" value="F:holo-[acyl-carrier-protein] synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.470.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008278">
        <property type="entry name" value="4-PPantetheinyl_Trfase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037143">
        <property type="entry name" value="4-PPantetheinyl_Trfase_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01648">
        <property type="entry name" value="ACPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56214">
        <property type="entry name" value="SSF56214"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="ACPS" evidence="2">
        <location>
            <begin position="85"/>
            <end position="149"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01648"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF11258.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="208" mass="22331" checksum="AC6F03125BE6D0B3" modified="2006-05-30" version="1">MSQEELGRLATMRAEARAEQFAAGRWLARRLLAVTFGGDASEWALSAAEDSPPLAIHTSGAVGVPVFVSIAHSGDHLACAVADVPVGIDIEHLQPRKHLDTLIEATTTEAERAALPELLRESDADARLVAFFQLWTLKEAWIKCHGGNLFQTMMGHVVEALPAPISQANAVTWRRDDAVLALAASAPVDGLGLPDATGWRLRERDAAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="70" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LF25</accession>
    <name>Q1LF25_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Lipoprotein transmembrane</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_4386</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11251.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518868.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4386"/>
    <dbReference type="EnsemblBacteria" id="ABF11251">
        <property type="protein sequence ID" value="ABF11251"/>
        <property type="gene ID" value="Rmet_4386"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4386"/>
    <dbReference type="eggNOG" id="COG4258">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017576_0_0_4"/>
    <dbReference type="OMA" id="HVMTLVL"/>
    <dbReference type="OrthoDB" id="1756450at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="259"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="283"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="310"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="343"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="378"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="429"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="642"/>
            <end position="660"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="667"/>
            <end position="687"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="693"/>
            <end position="714"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="721"/>
            <end position="742"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="748"/>
            <end position="769"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF11251.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="786" mass="84388" checksum="E2FE8F57F5B0B24D" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="103" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LF37</accession>
    <name>Q1LF37_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Homogentisate 1,2-dioxygenase</fullName>
            <shortName evidence="6">HGDO</shortName>
            <ecNumber evidence="6">1.13.11.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Homogentisate oxygenase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">Homogentisic acid oxidase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">Homogentisicase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6 7" type="primary">hmgA</name>
        <name evidence="7" type="ordered locus">Rmet_4374</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="8">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="8">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Involved in the catabolism of homogentisate (2,5-dihydroxyphenylacetate or 2,5-OH-PhAc), a central intermediate in the degradation of phenylalanine and tyrosine. Catalyzes the oxidative ring cleavage of the aromatic ring of homogentisate to yield maleylacetoacetate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>homogentisate + O2 = 4-maleylacetoacetate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:15449"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16169"/>
            <dbReference type="ChEBI" id="CHEBI:17105"/>
            <dbReference type="EC" id="1.13.11.5"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 6">
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="6">Amino-acid degradation; L-phenylalanine degradation; acetoacetate and fumarate from L-phenylalanine: step 4/6.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Hexamer; dimer of trimers.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the homogentisate dioxygenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="1.13.11.5" evidence="6"/>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11239.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518857.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4374"/>
    <dbReference type="EnsemblBacteria" id="ABF11239">
        <property type="protein sequence ID" value="ABF11239"/>
        <property type="gene ID" value="Rmet_4374"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4374"/>
    <dbReference type="eggNOG" id="COG3508">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027174_0_0_4"/>
    <dbReference type="OMA" id="FMFETRW"/>
    <dbReference type="OrthoDB" id="193687at2"/>
    <dbReference type="UniPathway" id="UPA00139">
        <property type="reaction ID" value="UER00339"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004411">
        <property type="term" value="F:homogentisate 1,2-dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006559">
        <property type="term" value="P:L-phenylalanine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006572">
        <property type="term" value="P:tyrosine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00334">
        <property type="entry name" value="Homogentis_dioxygen"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005708">
        <property type="entry name" value="Homogentis_dOase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022950">
        <property type="entry name" value="Homogentis_dOase_bac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011051">
        <property type="entry name" value="RmlC_Cupin_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11056">
        <property type="entry name" value="PTHR11056"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04209">
        <property type="entry name" value="HgmA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51182">
        <property type="entry name" value="SSF51182"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01015">
        <property type="entry name" value="hmgA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="6" id="KW-0408">Iron</keyword>
    <keyword evidence="3 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5 6" id="KW-0585">Phenylalanine catabolism</keyword>
    <keyword evidence="7" id="KW-0614">Plasmid</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0828">Tyrosine catabolism</keyword>
    <feature type="active site" description="Proton acceptor" evidence="6">
        <location>
            <position position="296"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="375"/>
        </location>
        <ligand>
            <name>homogentisate</name>
            <dbReference type="ChEBI" id="CHEBI:16169"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="394"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007757"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023232"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABF11239.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="442" mass="49060" checksum="76DBD48F3DA15C65" modified="2006-05-30" version="1">MTQTQLNVADTAVAEYMSGFANEFATEALPGALPVGRNSPQRAPYGLYAEQLSGTAFTAPRAHNRRSWLYRIRPAAMHKPFTLIEQDRWLSRFDEVAPSPNQMRWSAPAMPTVPTDFVDGIVTMAGNGGPEALSGCGIHLYLANASMRDRFFYNADGEMLIVPQEGRLLIVTEMGRLAVEPQEIVVIPRGVRFRVELPDGAARGYICENYGAMFKLPDLGVIGSNGLANPRDFLTPVASYEDREGDFELVARFQGNLWRADIGHSPLDVVAWHGNYVPYKYDLRLFNTIGSISYDHPDPSIFLVLQSPSDTPGVDTIDFVIFGPRWLAAEDTFRPPWFHRNIASEFMGLIAGEYDAKAEGFVPGGASLHNCMSGHGPDAETFERASASDTSKPHHITDTMAFMFETPGVIRPTRHAAESALLQHDYYTCWQGLKKHFNPNVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="100" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LFE5</accession>
    <name>Q1LFE5_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Transcriptional regulator, TetR/AcrR family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Rmet_4265</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="5">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="5">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11131.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF11131">
        <property type="protein sequence ID" value="ABF11131"/>
        <property type="gene ID" value="Rmet_4265"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4265"/>
    <dbReference type="eggNOG" id="COG1309">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069356_27_1_4"/>
    <dbReference type="OMA" id="LRIACEP"/>
    <dbReference type="OrthoDB" id="1437290at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001647">
        <property type="entry name" value="HTH_TetR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036271">
        <property type="entry name" value="Tet_transcr_reg_TetR-rel_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039536">
        <property type="entry name" value="TetR_C_Proteobacteria"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14246">
        <property type="entry name" value="TetR_C_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00440">
        <property type="entry name" value="TetR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00455">
        <property type="entry name" value="HTHTETR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48498">
        <property type="entry name" value="SSF48498"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50977">
        <property type="entry name" value="HTH_TETR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0614">Plasmid</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH tetR-type" evidence="3">
        <location>
            <begin position="33"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="H-T-H motif" evidence="1">
        <location>
            <begin position="56"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF11131.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="228" mass="24506" checksum="D2C9BED17AE29DC8" modified="2006-05-30" version="1">MVPTLPVKATSSPTLKSPASVEKPAMRITRRGEAKRAAILETAAMLFLARGYDGVSLDEIVKACGGSKTNVYSYFGGKDGLFVAVVEALCDRFLAKVEKLEPSRTNTANGLRDLASRFLESLLEESHLAFLRLIIAEARRFPEIGQVWYARGPLATCQYVAGYLARQQSTGTKLNATPEVAAQLFHSMVIAGVLHRTLATGEHPGEAQIDMLVKSAIDLIVAKSSAAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="72" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LFY1</accession>
    <name>Q1LFY1_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Putative TRAP transporter solute receptor, TAXI family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_4077</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10945.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4077"/>
    <dbReference type="EnsemblBacteria" id="ABF10945">
        <property type="protein sequence ID" value="ABF10945"/>
        <property type="gene ID" value="Rmet_4077"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4077"/>
    <dbReference type="eggNOG" id="COG2358">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030939_0_0_4"/>
    <dbReference type="OMA" id="PIWVFYR"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011852">
        <property type="entry name" value="TRAP_TAXI"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42941">
        <property type="entry name" value="PTHR42941"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16868">
        <property type="entry name" value="NMT1_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02122">
        <property type="entry name" value="TRAP_TAXI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="1" id="KW-0675">Receptor</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF10945.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="447" mass="49836" checksum="7BF26926F39629B0" modified="2006-05-30" version="1">MSRRDPALRRSQNPPMSAGRRRLLIAGGILLLVALGWSLVAVIRPAIQHTIIITTGADKGIYQGFADRYATILKRDGIKLEIRSSSGSIENYERLKNPDSAYEVGFIQSGTVSPSDTDGLQTIAAVAYEPIWVFYRGAAKMDRLSELQGKKVSIGVPGSGLLEVSRILLAHSGITASNTTLLQMDANAAYQGLKDGQLDAAFFIGRPDAEMQQTLLNSDLKLMSFAQADALVQKFPSLSKIVFPRASTSIVNDLPQADVTLLAAKALLVSKDTMHPALVYLLLEAAKTVHGGEDYFTPLGTFPNLNTNEFPVSEESTRFFKSGRPFLQRYLPFWLASFVERRLLILLPFMALLLGLLQALPRFAESRIKGRLVVWYREIKALEDEIWKSERPTRHQIAQWREEIEQIDAHASQIRMPQRYFQDVYALKQAIAVVRNRILHVAGTVKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LG67</accession>
    <name>Q1LG67_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Arsenical pump membrane protein, putative arsenite permease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">arsB1</name>
        <name evidence="7" type="ordered locus">Rmet_3991</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="8">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="8">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10859.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518499.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3991"/>
    <dbReference type="EnsemblBacteria" id="ABF10859">
        <property type="protein sequence ID" value="ABF10859"/>
        <property type="gene ID" value="Rmet_3991"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3991"/>
    <dbReference type="eggNOG" id="COG1055">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_043931_0_0_4"/>
    <dbReference type="OMA" id="AMTTVWL"/>
    <dbReference type="OrthoDB" id="626718at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015105">
        <property type="term" value="F:arsenite transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01118">
        <property type="entry name" value="ArsB_permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000802">
        <property type="entry name" value="Arsenical_pump_ArsB"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02040">
        <property type="entry name" value="ArsB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00758">
        <property type="entry name" value="ARSENICPUMP"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0614">Plasmid</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="31"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="61"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="99"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="140"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="179"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="220"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="246"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="276"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="318"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="370"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="399"/>
            <end position="418"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABF10859.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="419" mass="43666" checksum="A7C4E0AB9A216C2D" modified="2006-05-30" version="1">MPAYATPLIWSVAALSTAGVLFRPFRLPEPFWAMAGALVLCVAGLLPWRDALQAVARGNDVYLFLAGMILISELARKTGLFDHVAALAVRAARGSARKLFALVYGFGIAVTAFMSNDATAVVLTPAVIAATRAARVKHPLPYLYACAFIANAASFLLPISNPANLVLFGDRMPPLTSWLARFTLPSVVAIAMTFIVLYWTQRDALAEPIENDVPTPPLTLQAWLTTLGIMLTGAALLTASLHGQDLGWPTFIGGLLTLAVVCATQPRLLVPALKEVSWGVLPLVAGLFVLVAGLAQTGLTAQLAHWVRMLSGLQGPEAVLGAGVAGVLVGITSNIVNNLPAGLFAASALAAGHASDTVTAAVLIGVDLGPNLSITGSLATLLWLTALRREGHMVGAGTFLKTGALVMPLALLPALAVLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LGG1</accession>
    <name>Q1LGG1_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">MaoC_dehydrat_N domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_3897</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10765.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518412.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3897"/>
    <dbReference type="EnsemblBacteria" id="ABF10765">
        <property type="protein sequence ID" value="ABF10765"/>
        <property type="gene ID" value="Rmet_3897"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3897"/>
    <dbReference type="eggNOG" id="COG3777">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028690_3_0_4"/>
    <dbReference type="OMA" id="RMWAGGD"/>
    <dbReference type="OrthoDB" id="1056569at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029069">
        <property type="entry name" value="HotDog_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039569">
        <property type="entry name" value="MaoC-like_dehydrat_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13452">
        <property type="entry name" value="MaoC_dehydrat_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54637">
        <property type="entry name" value="SSF54637"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MaoC_dehydrat_N" evidence="1">
        <location>
            <begin position="77"/>
            <end position="138"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF10765.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="286" mass="31877" checksum="7902FF350B4A5D6F" modified="2006-05-30" version="1">MTTPTLEQLREWIGRTESRTETLAAEPVQGLAATFDLDGDALVSQPLPPLWHWLYFLPRAPQHEIGVDGHPALGGFLPPVPLPRRMWAGSEVTFKRGLRVGETVTKTSTITDVQHKSGRTGELWFVTVDHELSVNGETALNERHDIVYRAMPERGQPQPPRPRLAHTAQFGRSLKADPVLLFRYSALTFNGHRIHYDLDYVRDVEGYPGLIVHGPMQAMLMLELLRHERPDARVKRFGFRGLAPLFHLDTVTVGGMDDAETPGKVILWTGDDAGGQAMAGWAEVEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-10-07" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LGN1</accession>
    <name>Q1LGN1_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Rmet_3825</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="5">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="5">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the IMPACT family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10695.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3825"/>
    <dbReference type="EnsemblBacteria" id="ABF10695">
        <property type="protein sequence ID" value="ABF10695"/>
        <property type="gene ID" value="Rmet_3825"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3825"/>
    <dbReference type="eggNOG" id="COG1739">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_083552_0_0_4"/>
    <dbReference type="OMA" id="VTVDHQR"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035647">
        <property type="entry name" value="EFG_III/V"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023582">
        <property type="entry name" value="Impact"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001498">
        <property type="entry name" value="Impact_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036956">
        <property type="entry name" value="Impact_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015269">
        <property type="entry name" value="UPF0029_Impact_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16301">
        <property type="entry name" value="PTHR16301"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09186">
        <property type="entry name" value="DUF1949"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01205">
        <property type="entry name" value="UPF0029"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="SSF54211"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54980">
        <property type="entry name" value="SSF54980"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0614">Plasmid</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UPF0029" evidence="2">
        <location>
            <begin position="59"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="domain" description="DUF1949" evidence="3">
        <location>
            <begin position="177"/>
            <end position="235"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007665"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF09186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF10695.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="245" mass="26377" checksum="0CB4171139634300" modified="2006-05-30" version="1">MISTQHDSFLTVSKLSRDPVSVAAGRPALHSPPAQRPLHFPVLPTYTLAAPVHADIEIRKSRFIALAVPVADRDAAMAVIQDLRAQHPTATHVCWALLAGGQSGMSDDGEPSGTAGRPILEVLRHHDLDGVLAAVVRYYGGVKLGAGGLVRAYTDAIAGALKDAERIERIAYTTFAIEVDYADESRVRRWLELSAAEGCTLVDTRYDALATLLIRLPATLRDGAIATLRDATHGRARFPDTEEQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LGN4</accession>
    <name>Q1LGN4_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Probable membrane transporter protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Rmet_3822</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="7">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="7">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cell membrane</location>
            <topology evidence="5">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the 4-toluene sulfonate uptake permease (TSUP) (TC 2.A.102) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10692.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518338.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3822"/>
    <dbReference type="EnsemblBacteria" id="ABF10692">
        <property type="protein sequence ID" value="ABF10692"/>
        <property type="gene ID" value="Rmet_3822"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3822"/>
    <dbReference type="eggNOG" id="COG0730">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_054750_7_1_4"/>
    <dbReference type="OMA" id="RRCFFVG"/>
    <dbReference type="OrthoDB" id="1274094at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002781">
        <property type="entry name" value="TM_pro_TauE-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01925">
        <property type="entry name" value="TauE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0614">Plasmid</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="12"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="77"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="107"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="132"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="169"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="203"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="233"/>
            <end position="250"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU363041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABF10692.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="251" mass="26018" checksum="370B2A8EA53EC6E7" modified="2006-05-30" version="1">MTLFDIGQEIAFIGLTFLLAGFVKGVVGLGLPTVAVGLLGLVMPPAQAAALLVAPSMVTNVVQLFSGPRFGLLLQRLWPMLVAICAGTWICAALVPARVLGHATNALGVALVLYAIVGLAAVRLHVPPRAEGWLAPLIGLVTGAITAVTGVFVIPAVPYLQGLGLDKESMVQALGLSFTVSTIALAVSLALDGALLHTPVLGASALALVPALGGMFFGQWLRHRISAERFRRLFFCGLLLLGVDLALRGAF</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LGQ3</accession>
    <name>Q1LGQ3_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Putative acyl-CoA dehydrogenase-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Rmet_3803</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="9">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="9">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the acyl-CoA dehydrogenase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10673.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518322.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3803"/>
    <dbReference type="EnsemblBacteria" id="ABF10673">
        <property type="protein sequence ID" value="ABF10673"/>
        <property type="gene ID" value="Rmet_3803"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3803"/>
    <dbReference type="eggNOG" id="COG1960">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018204_9_0_4"/>
    <dbReference type="OMA" id="APTIMHF"/>
    <dbReference type="OrthoDB" id="760677at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016627">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-CH group of donors"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.540.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.110.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006091">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046373">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036250">
        <property type="entry name" value="AcylCo_DH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009075">
        <property type="entry name" value="AcylCo_DH/oxidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013786">
        <property type="entry name" value="AcylCoA_DH/ox_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037069">
        <property type="entry name" value="AcylCoA_DH/ox_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009100">
        <property type="entry name" value="AcylCoA_DH/oxidase_NM_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00441">
        <property type="entry name" value="Acyl-CoA_dh_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02770">
        <property type="entry name" value="Acyl-CoA_dh_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02771">
        <property type="entry name" value="Acyl-CoA_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47203">
        <property type="entry name" value="SSF47203"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56645">
        <property type="entry name" value="SSF56645"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0274">FAD</keyword>
    <keyword evidence="3" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="8" id="KW-0614">Plasmid</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Acyl-CoA_dh_N" evidence="7">
        <location>
            <begin position="6"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA_dh_M" evidence="6">
        <location>
            <begin position="125"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA_dh_1" evidence="5">
        <location>
            <begin position="231"/>
            <end position="393"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF02770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF02771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ABF10673.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="397" mass="44757" checksum="8D70B2C92AEAEE73" modified="2006-05-30" version="1">MDLRFTKEEEAFREEVRAFVQANLPTDIRDKVLGHRRVEKDDYVRWHRILHAHGWGAPTWPKEWGGTGWNALQRLIFEIETLRAGAPRMLPFGLTMIGPVLMKYASAAHKERFLPRIPTVDEFWCQGYSEPGSGSDLASLRTSAVRRGDKYIVNGQKTWTTMAHFADWIFCLVRTDPEAKAQEGISMLLIDMKSPGVTVRPIKTLDGGHDVNETWFEEVEVPVENLLGEENKGWTYAKYLLGHERTGIAGIGHCYRELRLLRKYAAETTDGRGGRLIDDVRMRDKIARLEMDLMALEMLLLRVATQAAGTPGPEASIVKIRGSELQQDIAMLQMEVAGPNGWPYSTDWMELGAPQPVSGAEWAAPAASTYYDMRKTTIYGGSTEVQKNIISKMIIGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="103" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LH89</accession>
    <name>Q1LH89_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Ribonuclease P protein component</fullName>
            <shortName evidence="6">RNase P protein</shortName>
            <shortName evidence="6">RNaseP protein</shortName>
            <ecNumber evidence="6">3.1.26.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Protein C5</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">rnpA</name>
        <name evidence="8" type="ordered locus">Rmet_3615</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 6">RNaseP catalyzes the removal of the 5'-leader sequence from pre-tRNA to produce the mature 5'-terminus. It can also cleave other RNA substrates such as 4.5S RNA. The protein component plays an auxiliary but essential role in vivo by binding to the 5'-leader sequence and broadening the substrate specificity of the ribozyme.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>Endonucleolytic cleavage of RNA, removing 5'-extranucleotides from tRNA precursor.</text>
            <dbReference type="EC" id="3.1.26.5"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="6">Consists of a catalytic RNA component (M1 or rnpB) and a protein subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the RnpA family.</text>
    </comment>
    <dbReference type="EC" id="3.1.26.5" evidence="6"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10487.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3615"/>
    <dbReference type="EnsemblBacteria" id="ABF10487">
        <property type="protein sequence ID" value="ABF10487"/>
        <property type="gene ID" value="Rmet_3615"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3615"/>
    <dbReference type="eggNOG" id="COG0594">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_717411_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004526">
        <property type="term" value="F:ribonuclease P activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001682">
        <property type="term" value="P:tRNA 5'-leader removal"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00227">
        <property type="entry name" value="RNase_P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000100">
        <property type="entry name" value="RNase_P"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020539">
        <property type="entry name" value="RNase_P_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33992">
        <property type="entry name" value="PTHR33992"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00825">
        <property type="entry name" value="Ribonuclease_P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="SSF54211"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00188">
        <property type="entry name" value="rnpA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00648">
        <property type="entry name" value="RIBONUCLEASE_P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="5 6 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 6" id="KW-0540">Nuclease</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="2 6" id="KW-0819">tRNA processing</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="89"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="163"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="207"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="366"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="99"/>
            <end position="113"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00227"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ABF10487.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="385" mass="41814" checksum="720F1F89796C7809" modified="2006-05-30" version="1">MTGNTHRVTHCDLWTMAHHVGVTDSPPPPASTIGRLDRLNEYRLNGGFCDAAGDHVCASPGLARTVRVNHVRHQASGLIPAGASSNVAMPAAAGSKTEPESSARPQLSQSLHRGTAPCEPYAAAKAPRQIAGARKARINQRVQHETYLSTFRYPPQAYPWFPRAHEDPWRPCRDQRTPRQGPQAPGDLSAGRPARAVNVGLRDSRCLDESLPGQAPRTQDGREHCPSKPSLPLALPSVSNHAFPKAARLTKTDEFSSVFALRPRRRTTHFVLYVRANGQAQARLGIVVGKKFAPRAAERNLVKRMVRELFRSRQAQFAGRDILLRLQAKFPRAEFSSRVAIRKACHAEISGLLDVAAKPLPVAPAVPTAPPTASADVPTTPEAPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="69" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LHC1</accession>
    <name>Q1LHC1_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Cofac_haem_bdg domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_3583</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10455.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517997.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3583"/>
    <dbReference type="EnsemblBacteria" id="ABF10455">
        <property type="protein sequence ID" value="ABF10455"/>
        <property type="gene ID" value="Rmet_3583"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3583"/>
    <dbReference type="eggNOG" id="COG3016">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_062196_0_0_4"/>
    <dbReference type="OMA" id="VGEKHDN"/>
    <dbReference type="OrthoDB" id="1240854at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd14727">
        <property type="entry name" value="ChanN-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007314">
        <property type="entry name" value="Cofac_haem-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016773">
        <property type="entry name" value="Fe3_uptake_reg_CjrA_prd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04187">
        <property type="entry name" value="Cofac_haem_bdg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF020419">
        <property type="entry name" value="Fe_uptake_reg_CjrA_prd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193500" description="Cofac_haem_bdg domain-containing protein" evidence="1">
        <location>
            <begin position="21"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="domain" description="Cofac_haem_bdg" evidence="2">
        <location>
            <begin position="29"/>
            <end position="231"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF04187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF10455.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="278" mass="30030" checksum="99557AD721C854F6" modified="2006-05-30" version="1">MNRFVACIAMAALTAGCASTGPSADALSQRFPNQTYVLLGEVHDNAAGQRQRLQALTQAVEAGWRPAIAMEQFDRERQGDIDRARRERPGDADYVIAQATGPKSGWNWALYQPVVSLALQYDLPLLAANLSRADAAKIVRGGLGQVFAPQQQAELKLDKPLPADLVALQTATLDAGHCGNFPKAMLPGMLDAQAARDAVMAQIMKAHGDRGVVLIAGNGHVRRDIGVPRWLDRADRDDVRVLSVGYAESEVNDKAFDVLVRVPAVDRPDPCTDAVMKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="96" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LHE1</accession>
    <name>Q1LHE1_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Protease HtpX homolog</fullName>
            <ecNumber evidence="12">3.4.24.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">htpX</name>
        <name evidence="14" type="ordered locus">Rmet_3563</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="12">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="12">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="12">Cell inner membrane</location>
            <topology evidence="12">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the peptidase M48B family.</text>
    </comment>
    <dbReference type="EC" id="3.4.24.-" evidence="12"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10435.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008641022.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3563"/>
    <dbReference type="EnsemblBacteria" id="ABF10435">
        <property type="protein sequence ID" value="ABF10435"/>
        <property type="gene ID" value="Rmet_3563"/>
    </dbReference>
    <dbReference type="GeneID" id="60822668"/>
    <dbReference type="KEGG" id="rme:Rmet_3563"/>
    <dbReference type="eggNOG" id="COG0501">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_042266_3_0_4"/>
    <dbReference type="OMA" id="REYMADS"/>
    <dbReference type="OrthoDB" id="1918093at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00188">
        <property type="entry name" value="Pept_M48_protease_HtpX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022919">
        <property type="entry name" value="Pept_M48_protease_HtpX"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001915">
        <property type="entry name" value="Peptidase_M48"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01435">
        <property type="entry name" value="Peptidase_M48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="3 12" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10 12" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="4 12" id="KW-0645">Protease</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="8 12" id="KW-0862">Zinc</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="7"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="31"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="141"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="177"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase_M48" evidence="13">
        <location>
            <begin position="66"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="active site" evidence="12">
        <location>
            <position position="132"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="131"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="135"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="202"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009779"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF01435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ABF10435.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="286" mass="30833" checksum="C7C134572559B686" modified="2006-05-30" version="1">MFNWVKTFMLMAAITALFVVIGGMIGGRNGMTFALLIALGMNFFSYWFSDKMVLRMYNAQEVDASTAPQFYNMVHELSQRAGLPMPRVYLINEDAPNAFATGRNPEHAAVAATTGILRVLSDRELRGVMAHELAHVKHRDILTSTIAATMAGAISALANMAMFFGGRDENGNRSNPIAAIAVAILAPLAASLIQMAISRAREFEADRGGAEISGDPQALASALDKIHRYAQGIPFQAAEEHPATAQMMIMNPLSGGGIANLFSTHPATEERIARLMQMAQTGTYPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="72" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LHJ6</accession>
    <name>Q1LHJ6_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Extra-cytoplasmic Solute Receptor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">bug</name>
        <name evidence="4" type="ordered locus">Rmet_3508</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Periplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the UPF0065 (bug) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10380.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3508"/>
    <dbReference type="EnsemblBacteria" id="ABF10380">
        <property type="protein sequence ID" value="ABF10380"/>
        <property type="gene ID" value="Rmet_3508"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3508"/>
    <dbReference type="eggNOG" id="COG3181">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045683_0_0_4"/>
    <dbReference type="OMA" id="KEMGDGG"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042597">
        <property type="term" value="C:periplasmic space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005064">
        <property type="entry name" value="BUG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042100">
        <property type="entry name" value="Bug_dom1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42928">
        <property type="entry name" value="PTHR42928"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03401">
        <property type="entry name" value="TctC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017082">
        <property type="entry name" value="YflP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0675">Receptor</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193548" evidence="3">
        <location>
            <begin position="20"/>
            <end position="320"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF10380.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="320" mass="33322" checksum="B7EEA1CF13B45301" modified="2006-05-30" version="1">MSAAVGTLATVGLTASAFAQSNYPNKPIRLVVPFAAGGSTDLSARLVAEFAGRELGQTIVVDNKGGAGGSIGMEQVARATPDGYTIGMATVSTHGSNPAVYPKLGYDPIKDFAPVTNVVAIPSVFAVHPSVPAKTMQEFIALAKAHPGKYTFASPGAGSLGHVNIENFMMLAKIDLLHVPYKGAGLALNDAVAGQVNAITDNLSSTLPHVKAGRLRALAVLGTQRSAQLPNVPTYAELGFKDMGEGGWFGIVAPAGTPQPVIDKLNAAIHKAMQNPEFKRKVEESGGTLVPTTPDQFKAQIQQSIARYARVVKAANIKLD</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2007-09-11" modified="2022-05-25" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LIB8</accession>
    <name>TATB_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Sec-independent protein translocase protein TatB</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">tatB</name>
        <name type="ordered locus">Rmet_3236</name>
    </gene>
    <organism>
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Part of the twin-arginine translocation (Tat) system that transports large folded proteins containing a characteristic twin-arginine motif in their signal peptide across membranes. Together with TatC, TatB is part of a receptor directly interacting with Tat signal peptides. TatB may form an oligomeric binding site that transiently accommodates folded Tat precursor proteins before their translocation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">The Tat system comprises two distinct complexes: a TatABC complex, containing multiple copies of TatA, TatB and TatC subunits, and a separate TatA complex, containing only TatA subunits. Substrates initially bind to the TatABC complex, which probably triggers association of the separate TatA complex to form the active translocon.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell inner membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the TatB family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10108.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517717.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q1LIB8"/>
    <dbReference type="SMR" id="Q1LIB8"/>
    <dbReference type="STRING" id="266264.Rmet_3236"/>
    <dbReference type="EnsemblBacteria" id="ABF10108">
        <property type="protein sequence ID" value="ABF10108"/>
        <property type="gene ID" value="Rmet_3236"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3236"/>
    <dbReference type="eggNOG" id="COG1826">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_086034_1_1_4"/>
    <dbReference type="OMA" id="ARPLWFK"/>
    <dbReference type="OrthoDB" id="2073616at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005887">
        <property type="term" value="C:integral component of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033281">
        <property type="term" value="C:TAT protein transport complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008320">
        <property type="term" value="F:protein transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043953">
        <property type="term" value="P:protein transport by the Tat complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00237">
        <property type="entry name" value="TatB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003369">
        <property type="entry name" value="TatA/B/E"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018448">
        <property type="entry name" value="TatB"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02416">
        <property type="entry name" value="TatA_B_E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01410">
        <property type="entry name" value="tatB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0997">Cell inner membrane</keyword>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-0653">Protein transport</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0811">Translocation</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <keyword id="KW-0813">Transport</keyword>
    <feature type="chain" id="PRO_0000301218" description="Sec-independent protein translocase protein TatB">
        <location>
            <begin position="1"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="92"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="146"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="92"/>
            <end position="113"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="168" mass="18812" checksum="72E1CCD73D02DBCF" modified="2006-05-30" version="1">MIDLGISKLALIGAVALIVIGPERLPKVARTVGALVGRAQRYINDVKAEVSREVELEELRKMRTEFENAARDVEQTIHKEVSEHTQALNEAFDGSASSSSSSDTGSGYVPSWDSAHKSHNGRKSWRVKQGARPIWFKRQQNTRMWVQSGAARVKRHRPASGRNRSFFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LID4</accession>
    <name>Q1LID4_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Predicted membrane bound metallophosphoesterase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_3220</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10092.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517703.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3220"/>
    <dbReference type="EnsemblBacteria" id="ABF10092">
        <property type="protein sequence ID" value="ABF10092"/>
        <property type="gene ID" value="Rmet_3220"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3220"/>
    <dbReference type="eggNOG" id="COG1408">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025443_5_1_4"/>
    <dbReference type="OMA" id="MQFGIEI"/>
    <dbReference type="OrthoDB" id="1690667at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP_ApaH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="SSF56300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="36"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="70"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="110"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="domain" description="Metallophos" evidence="2">
        <location>
            <begin position="154"/>
            <end position="322"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF10092.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="380" mass="41123" checksum="EAFB221F3CE09A56" modified="2006-05-30" version="1">MSAIRSRRSLLIPVLIVALLHVYIGVRLLPGLPVPMIVHSLAVLWLIASTLLMPAALMARRLSTPWADQLSWVGMMAMGFFSSLLVLTIARDLFLLVTLAIGWRPPGLTGYSALGAIALALAVTVIGFFQARGLAKVKEVDVPIANLPDALHGFTIAQISDIHVGPTIKRPYIDRIVDRVNSLAPDAIAITGDLVDGSVRELSAHTAPFARLSARHGAYVVTGNHEYYSGAEAWIVELRRLGLRVLMNEHVVVERGDASLVLGGVTDFTAGGFIESHRSDPVQALDGAPRDAAARVLLAHQPRTAPAAAAAGFDLQLSGHTHGGQFWPWNLFVPMQQPYVHGLNRHDNLWVYVSRGTGYWGPPKRFGAPSEITLVRLVRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LIE6</accession>
    <name>Q1LIE6_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative saccharopine dehydrogenase (NADP+,L-glutamate-forming)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_3208</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10080.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517689.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3208"/>
    <dbReference type="EnsemblBacteria" id="ABF10080">
        <property type="protein sequence ID" value="ABF10080"/>
        <property type="gene ID" value="Rmet_3208"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3208"/>
    <dbReference type="eggNOG" id="COG1748">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032858_0_0_4"/>
    <dbReference type="OMA" id="PCEAIVD"/>
    <dbReference type="OrthoDB" id="1006830at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032095">
        <property type="entry name" value="Sacchrp_dh-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005097">
        <property type="entry name" value="Sacchrp_dh_NADP"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16653">
        <property type="entry name" value="Sacchrp_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03435">
        <property type="entry name" value="Sacchrp_dh_NADP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Sacchrp_dh_NADP" evidence="1">
        <location>
            <begin position="23"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="domain" description="Sacchrp_dh_C" evidence="2">
        <location>
            <begin position="139"/>
            <end position="376"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF16653"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF10080.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="397" mass="42458" checksum="1220FC5E3157AC82" modified="2006-05-30" version="1">MQPSTLGPAAVRDLTLAPRPLSVVVLGAGKIGRVIAAMLAASGDYRVCLVDHDAHRLDGMPRQIAVRAGDPTEPGTCAALLAGADAVLNALPFHAAVNVAKVAARLGVHYFDLTEDVAATRVIRELGRSARAVLMPQCGLAPGFIGVVGHDLAQRFLRGGGELLDLRMRVGALPRYPSNALKYNLTWSTEGLINEYCNPCEAIVDGRRVELPALEGHETFALDGVEYEAFNTSGGLGTLPDTLAGHARQVDYKSIRYPGHCAQMKLLLNDLRLRERREWLREIFEHAIPVTEQDVVIVFASATGHPPGVRGEGKRGPLTQASFSARIGGMADFAGIGHVNAIQLTTAAGICTALDLVATGVLPQAGFVPQEAMPLDRFLANRFGQHYSHHPLQETLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="61" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LIF5</accession>
    <name>Q1LIF5_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_3199</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10071.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517680.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3199"/>
    <dbReference type="EnsemblBacteria" id="ABF10071">
        <property type="protein sequence ID" value="ABF10071"/>
        <property type="gene ID" value="Rmet_3199"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3199"/>
    <dbReference type="eggNOG" id="COG2261">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_160040_3_0_4"/>
    <dbReference type="OMA" id="GWIGPLW"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="24"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="56"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF10071.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="80" mass="7943" checksum="9C1C745210996C79" modified="2006-05-30" version="1">MLGLAVVGAVVGLAGRQMHPSGRVVSLPAALLLGAAGALAAFYTGRAAHLFTDGQLLGWGAAIIGAAVLVGVWGVARPRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LIQ9</accession>
    <name>Q1LIQ9_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Hypoxanthine phosphoribosyltransferase</fullName>
            <ecNumber evidence="3">2.4.2.8</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">hpt</name>
        <name evidence="3" type="ordered locus">Rmet_3095</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.4.2.8" evidence="3"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09967.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517595.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3095"/>
    <dbReference type="EnsemblBacteria" id="ABF09967">
        <property type="protein sequence ID" value="ABF09967"/>
        <property type="gene ID" value="Rmet_3095"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3095"/>
    <dbReference type="eggNOG" id="COG0634">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_073615_2_0_4"/>
    <dbReference type="OMA" id="TMDWMAV"/>
    <dbReference type="OrthoDB" id="1819460at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052657">
        <property type="term" value="F:guanine phosphoribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004422">
        <property type="term" value="F:hypoxanthine phosphoribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd06223">
        <property type="entry name" value="PRTases_typeI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2020">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000836">
        <property type="entry name" value="PRibTrfase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029057">
        <property type="entry name" value="PRTase-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00156">
        <property type="entry name" value="Pribosyltran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53271">
        <property type="entry name" value="SSF53271"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="41"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="domain" description="Pribosyltran" evidence="2">
        <location>
            <begin position="16"/>
            <end position="165"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF09967.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="180" mass="20299" checksum="AB41008C9829D232" modified="2006-05-30" version="1">MMTAEQARELWLDSEEIVSAEEVQNSLDRMAHDITEKMGDAFPLVLSVMGGAVVFTGMLLPKLQFPLEFDYIHLSRYNNKTVGGEMQWRVAPRESVKGRVVLVLDDILDEGETMAAIRQRIMDMGAKECHAAVLCEKTLTKIKPMHPDFCGFQVPDRYVFGCGMDVKGYWRNLGAIRALV</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="53" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LIW1</accession>
    <name>Q1LIW1_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_3043</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09915.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_003056223.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3043"/>
    <dbReference type="EnsemblBacteria" id="ABF09915">
        <property type="protein sequence ID" value="ABF09915"/>
        <property type="gene ID" value="Rmet_3043"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3043"/>
    <dbReference type="PATRIC" id="fig|402626.5.peg.3734"/>
    <dbReference type="eggNOG" id="ENOG502Z8ED">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_093433_0_0_4"/>
    <dbReference type="OMA" id="GFRRNLW"/>
    <dbReference type="OrthoDB" id="1068088at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="18"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="44"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="168"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="202"/>
            <end position="224"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF09915.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="262" mass="28626" checksum="6CB375477613ED53" modified="2006-05-30" version="1">MTTIFELVKDPYERKARVIPGLLVALPVLVPLLCVYGARHPVLTGVIGLLGGCGAIYALASVARGRGKKLEEMLVSKWGGMPTTIALRHRDKFLDGVSKQRYHTAITAKLGIAMPTAEEESANPDKADDIYIGATKRLRELTRSNKQLLLKENIAYGFHRNMLAMKPVGILSCLMGVVYGLLIAKILQVAPLQFDLVHLADPGLAAGLTLLISLALLAAWLLYFDQDAVRRMGFVYAERLFECLPSLPSSSSRKKAVKPTTD</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="56" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LIW8</accession>
    <name>Q1LIW8_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_3036</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09908.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_003056213.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF09908">
        <property type="protein sequence ID" value="ABF09908"/>
        <property type="gene ID" value="Rmet_3036"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3036"/>
    <dbReference type="PATRIC" id="fig|402626.5.peg.3742"/>
    <dbReference type="eggNOG" id="ENOG502ZSR6">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2317941_0_0_4"/>
    <dbReference type="OMA" id="ALIWTTD"/>
    <dbReference type="OrthoDB" id="1811398at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF09908.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="99" mass="10938" checksum="5623F825139182AD" modified="2006-05-30" version="1">MSDPMQPGTPAPGAEGPGIFLPTLIWTTDRKTVGNEMQRLLGRRAQLNVLLSASEETDDGTTWYAMAQATLNQLDCDIERLFEWLGDYEPDTPTPEVPS</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2017-05-10" modified="2022-08-03" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LJ80</accession>
    <name>ATR_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Cobalamin adenosyltransferase</fullName>
            <shortName evidence="4">ATR</shortName>
            <ecNumber evidence="3">2.5.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">cobO</name>
        <name evidence="7" type="ordered locus">Rmet_2923</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2017" name="J. Biol. Chem." volume="292" first="3977" last="3987">
            <title>Cofactor editing by the G-protein metallochaperone domain regulates the radical B12 enzyme IcmF.</title>
            <authorList>
                <person name="Li Z."/>
                <person name="Kitanishi K."/>
                <person name="Twahir U.T."/>
                <person name="Cracan V."/>
                <person name="Chapman D."/>
                <person name="Warncke K."/>
                <person name="Banerjee R."/>
            </authorList>
            <dbReference type="PubMed" id="28130442"/>
            <dbReference type="DOI" id="10.1074/jbc.M117.775957"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>CATALYTIC ACTIVITY</scope>
        <scope>BIOPHYSICOCHEMICAL PROPERTIES</scope>
        <scope>SUBUNIT</scope>
        <scope>GTP/GDP-BINDING</scope>
        <scope>ACTIVITY REGULATION</scope>
    </reference>
    <comment type="function">
        <text evidence="3">Adenosyltransferase that catalyzes the conversion of cob(II)alamin to adenosylcob(III)alamin (AdoCbl) in the presence of ATP and an electron donor. Acts as an accessory protein of IcmF that functions in cofactor repair, since IcmF is prone to inactivation during catalytic turnover due to the occasional loss of the 5'-deoxyadenosine moiety and formation of the inactive cob(II)alamin cofactor in its active site. Thus, receives and repairs the inactive cofactor, which is then reloaded onto IcmF in a GTPase-gated step.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>AH2 + 2 ATP + 2 cob(II)alamin = A + 2 adenosylcob(III)alamin + 2 H(+) + 2 triphosphate</text>
            <dbReference type="Rhea" id="RHEA:53304"/>
            <dbReference type="ChEBI" id="CHEBI:13193"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16304"/>
            <dbReference type="ChEBI" id="CHEBI:17499"/>
            <dbReference type="ChEBI" id="CHEBI:18036"/>
            <dbReference type="ChEBI" id="CHEBI:18408"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </reaction>
    </comment>
    <comment type="activity regulation">
        <text evidence="3">Is potentially allosterically regulated by GTP/GDP, which enhances its affinity for AdoCbl by 5-fold. Binds cob(II)alamin weakly in the absence of ATP. The presence of ATP (but not GTP or GDP) increases the affinity of cob(II)alamin for the enzyme, and stoichiometric binding is observed. GTP blocks the transfer of cob(II)alamin to IcmF from ATR, thus averting its reconstitution with inactive cofactor.</text>
    </comment>
    <comment type="biophysicochemical properties">
        <kinetics>
            <KM evidence="3">2.3 uM for ATP</KM>
            <text evidence="3">kcat is 3.8 min(-1).</text>
        </kinetics>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homotrimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the Cob(I)alamin adenosyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.-" evidence="3"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09796.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517468.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q1LJ80"/>
    <dbReference type="SMR" id="Q1LJ80"/>
    <dbReference type="STRING" id="266264.Rmet_2923"/>
    <dbReference type="PRIDE" id="Q1LJ80"/>
    <dbReference type="EnsemblBacteria" id="ABF09796">
        <property type="protein sequence ID" value="ABF09796"/>
        <property type="gene ID" value="Rmet_2923"/>
    </dbReference>
    <dbReference type="GeneID" id="60820641"/>
    <dbReference type="KEGG" id="rme:Rmet_2923"/>
    <dbReference type="eggNOG" id="COG2096">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_083486_0_1_4"/>
    <dbReference type="OMA" id="HQACTVV"/>
    <dbReference type="OrthoDB" id="1401910at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0000314"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019003">
        <property type="term" value="F:GDP binding"/>
        <property type="evidence" value="ECO:0000314"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0000314"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016765">
        <property type="term" value="F:transferase activity, transferring alkyl or aryl (other than methyl) groups"/>
        <property type="evidence" value="ECO:0000314"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009236">
        <property type="term" value="P:cobalamin biosynthetic process"/>
        <property type="evidence" value="ECO:0000314"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070207">
        <property type="term" value="P:protein homotrimerization"/>
        <property type="evidence" value="ECO:0000314"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030091">
        <property type="term" value="P:protein repair"/>
        <property type="evidence" value="ECO:0000314"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1200.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016030">
        <property type="entry name" value="CblAdoTrfase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036451">
        <property type="entry name" value="CblAdoTrfase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029499">
        <property type="entry name" value="PduO-typ"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12213">
        <property type="entry name" value="PTHR12213"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01923">
        <property type="entry name" value="Cob_adeno_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89028">
        <property type="entry name" value="SSF89028"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00636">
        <property type="entry name" value="PduO_Nterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0169">Cobalamin biosynthesis</keyword>
    <keyword id="KW-0342">GTP-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000439947" description="Cobalamin adenosyltransferase">
        <location>
            <begin position="1"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="10"/>
            <end position="13"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="18"/>
            <end position="19"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="28"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="130"/>
            <end position="134"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="154"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1">
        <source>
            <dbReference type="UniProtKB" id="Q96EY8"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="3">
        <source>
            <dbReference type="PubMed" id="28130442"/>
        </source>
    </evidence>
    <evidence type="ECO:0000303" key="4">
        <source>
            <dbReference type="PubMed" id="28130442"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="5"/>
    <evidence type="ECO:0000305" key="6">
        <source>
            <dbReference type="PubMed" id="28130442"/>
        </source>
    </evidence>
    <evidence type="ECO:0000312" key="7">
        <source>
            <dbReference type="EMBL" id="ABF09796.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ABF09796.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="184" mass="19904" checksum="9C8C8EDF9340F3EB" modified="2006-05-30" version="1">MGNRLSKIATRTGDAGTTGLGDGSRVGKNSLRIVAIGDVDELNSHIGLLLTEPDLPEDVRAALLHIQHDLFDLGGELSIPGYTLLKAPQVAQLDDWLAHYNAALPRLAEFILPGGSRPAAQAHICRTVCRRAERALVELGAAEALNEAPRQYLNRLSDLLFVLARVLNRAGGGSDVLWQRERES</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-04-07" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LJE3</accession>
    <name>Q1LJE3_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Nucleoside-diphosphate-sugar epimerase</fullName>
            <ecNumber evidence="4">5.1.3.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Rmet_2860</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EC" id="5.1.3.-" evidence="4"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09733.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2860"/>
    <dbReference type="EnsemblBacteria" id="ABF09733">
        <property type="protein sequence ID" value="ABF09733"/>
        <property type="gene ID" value="Rmet_2860"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2860"/>
    <dbReference type="eggNOG" id="COG0451">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007383_11_4_4"/>
    <dbReference type="OMA" id="MGDYFDL"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016853">
        <property type="term" value="F:isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006813">
        <property type="term" value="P:potassium ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003148">
        <property type="entry name" value="RCK_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029903">
        <property type="entry name" value="RmlD-like-bd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04321">
        <property type="entry name" value="RmlD_sub_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02254">
        <property type="entry name" value="TrkA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0406">Ion transport</keyword>
    <keyword evidence="4" id="KW-0413">Isomerase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="RCK N-terminal" evidence="2">
        <location>
            <begin position="16"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="domain" description="RmlD_sub_bind" evidence="3">
        <location>
            <begin position="142"/>
            <end position="280"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF04321"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF09733.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="331" mass="37388" checksum="6FAA1E52DAB07CA9" modified="2006-05-30" version="1">MSSPRGARRLGRPRLLIVGCGDVGTRILRLLSARMRVFAVTSQPERRAELRAAGAVPLVADLDRPATLARLSRLAERVIDLAPPPGQGEGDPRTQGLLGALRRTAWRRARFTRLPTGAGEAAILPEQGWHRAFQTARRHQSRGVFVYASTTGVYGDRGGARVREFDAVRPQTARARRRVAAEAAVRRFGRDAGWRAGIVRIPGIYAEDRLPIARLNKRTPALASQDDVYTSHIHADDLARTMIAAMWRGRPQRVVHASDDTELRMADYFDLVADRKDLPRPPRITRQQAREMIEPTLLSFMSESRRIDNRRLKRELRLRLRYPTVASFFDR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-02-26" modified="2022-05-25" version="92" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LJR3</accession>
    <name>PYRB_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Aspartate carbamoyltransferase</fullName>
            <ecNumber evidence="1">2.1.3.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Aspartate transcarbamylase</fullName>
            <shortName evidence="1">ATCase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">pyrB</name>
        <name type="ordered locus">Rmet_2740</name>
    </gene>
    <organism>
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>carbamoyl phosphate + L-aspartate = H(+) + N-carbamoyl-L-aspartate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:20013"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
            <dbReference type="ChEBI" id="CHEBI:32814"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58228"/>
            <dbReference type="EC" id="2.1.3.2"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Pyrimidine metabolism; UMP biosynthesis via de novo pathway; (S)-dihydroorotate from bicarbonate: step 2/3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the aspartate/ornithine carbamoyltransferase superfamily. ATCase family.</text>
    </comment>
    <dbReference type="EC" id="2.1.3.2" evidence="1"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09613.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008646281.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q1LJR3"/>
    <dbReference type="SMR" id="Q1LJR3"/>
    <dbReference type="STRING" id="266264.Rmet_2740"/>
    <dbReference type="EnsemblBacteria" id="ABF09613">
        <property type="protein sequence ID" value="ABF09613"/>
        <property type="gene ID" value="Rmet_2740"/>
    </dbReference>
    <dbReference type="GeneID" id="60820809"/>
    <dbReference type="KEGG" id="rme:Rmet_2740"/>
    <dbReference type="eggNOG" id="COG0540">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_043846_2_0_4"/>
    <dbReference type="OMA" id="FPTEREY"/>
    <dbReference type="OrthoDB" id="1844275at2"/>
    <dbReference type="UniPathway" id="UPA00070">
        <property type="reaction ID" value="UER00116"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016597">
        <property type="term" value="F:amino acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004070">
        <property type="term" value="F:aspartate carbamoyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006207">
        <property type="term" value="P:'de novo' pyrimidine nucleobase biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044205">
        <property type="term" value="P:'de novo' UMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006520">
        <property type="term" value="P:cellular amino acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1370">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00001">
        <property type="entry name" value="Asp_carb_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006132">
        <property type="entry name" value="Asp/Orn_carbamoyltranf_P-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006130">
        <property type="entry name" value="Asp/Orn_carbamoylTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036901">
        <property type="entry name" value="Asp/Orn_carbamoylTrfase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002082">
        <property type="entry name" value="Asp_carbamoyltransf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006131">
        <property type="entry name" value="Asp_carbamoyltransf_Asp/Orn-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11405:SF16">
        <property type="entry name" value="PTHR11405:SF16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00185">
        <property type="entry name" value="OTCace"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02729">
        <property type="entry name" value="OTCace_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00100">
        <property type="entry name" value="AOTCASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53671">
        <property type="entry name" value="SSF53671"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00670">
        <property type="entry name" value="asp_carb_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00097">
        <property type="entry name" value="CARBAMOYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0665">Pyrimidine biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000321147" description="Aspartate carbamoyltransferase">
        <location>
            <begin position="1"/>
            <end position="323"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00001"/>
        </source>
    </evidence>
    <sequence length="323" mass="35248" checksum="1BAE62504717E8A1" modified="2006-05-30" version="1">MTKTFRNPQLTKNGELKHLLSIEGLSRDMITHILDTASQFVSLSDSDREVKKVPLLRGKSVFNLFFENSTRTRTTFEIAAKRLSADVLNLNINASSTSKGESLLDTINNLSAMSADMFVVRHASSGAPYLIAEHVAPHVHVINAGDGRHAHPTQGLLDMYTIRHFKKDFTNLTVAIVGDILHSRVARSDIHALTTLGVPEVRAIGPRTLLPSGLEQMGVRVFHNMEEGLKGVDVVIMLRLQNERMTGALLPSAQEYFKAYGLTPERLALAKPDAIVMHPGPMNRGVEIDSAVADGPQSVILNQVTFGIAVRMAVMGIVAGNND</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LJU5</accession>
    <name>Q1LJU5_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Transcriptional regulator, TetR/AcrR-family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_2708</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09581.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2708"/>
    <dbReference type="EnsemblBacteria" id="ABF09581">
        <property type="protein sequence ID" value="ABF09581"/>
        <property type="gene ID" value="Rmet_2708"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2708"/>
    <dbReference type="eggNOG" id="COG1309">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069356_27_2_4"/>
    <dbReference type="OMA" id="KLIMAES"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001647">
        <property type="entry name" value="HTH_TetR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036271">
        <property type="entry name" value="Tet_transcr_reg_TetR-rel_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011075">
        <property type="entry name" value="TetR_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16859">
        <property type="entry name" value="TetR_C_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00440">
        <property type="entry name" value="TetR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00455">
        <property type="entry name" value="HTHTETR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48498">
        <property type="entry name" value="SSF48498"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50977">
        <property type="entry name" value="HTH_TETR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH tetR-type" evidence="2">
        <location>
            <begin position="32"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="H-T-H motif" evidence="1">
        <location>
            <begin position="55"/>
            <end position="74"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF09581.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="266" mass="29467" checksum="1D08E78409D14122" modified="2006-05-30" version="1">MLRPRALPFPHTVTAALPKKDPDTRRWSRRKAARPQELVAAALDLFVERGYAATRLEDVAAAAGVSKGTVYLYFANKEELFKTVVRENMVPALVRGTDLVDRYEGSTPELLRELLLGWWGLIGATPVAGLTKLIMAESHNFPDIAQFYQDEVMGPGDELFARVLARGVARGEFRAAPANPTTTLICAPLVFLMLWRSAFGQLDCRVIDPEAFVDQLLQVLLFGLTTGPSRDMPLPPVQGPYIWERIRDELLAERAADLAPPAETGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="50" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LKA1</accession>
    <name>Q1LKA1_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_2548</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09425.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517127.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF09425">
        <property type="protein sequence ID" value="ABF09425"/>
        <property type="gene ID" value="Rmet_2548"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2548"/>
    <dbReference type="HOGENOM" id="CLU_2002008_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="102"/>
            <end position="124"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF09425.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="124" mass="13401" checksum="E1F5570DFC3E66D3" modified="2006-05-30" version="1">MRAALPAIPVSIVIPVIAPITISVIVISIVIRSTISAVIPTASVIAMIGGNDAPTEQRHGGDEKHKNGLHMALLFSLFLSRAYALDYRHDVGARTFRSKAFSYSERRRGAKRSEETIKGEASAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="61" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LKF8</accession>
    <name>Q1LKF8_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_2491</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09368.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517070.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2491"/>
    <dbReference type="EnsemblBacteria" id="ABF09368">
        <property type="protein sequence ID" value="ABF09368"/>
        <property type="gene ID" value="Rmet_2491"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2491"/>
    <dbReference type="eggNOG" id="ENOG5031T51">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_118489_1_0_4"/>
    <dbReference type="OMA" id="TGADRFH"/>
    <dbReference type="OrthoDB" id="1961863at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193236" evidence="2">
        <location>
            <begin position="24"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="27"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="159"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="169"/>
            <end position="183"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF09368.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="183" mass="19218" checksum="58D011AEFB7F7325" modified="2006-05-30" version="1">MKHIVLAACLGALTLTSAGAALAQEPAKKPAATKKTASKKTTKKTEAAAPVEAPAGEKWQCELGHALYITGNMQRDEVIGVNWEGKVYRLPRQNTVTGADRYFDQRTGMDLVVIPSKAMLFNRNLGQRLADECQTADMQAGGSAPTVAGALRAPVTTPLLVAPSQPAQPADAQPASAQSAPKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="96" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LKI6</accession>
    <name>Q1LKI6_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Dihydrofolate synthase/folylpolyglutamate synthase</fullName>
            <ecNumber evidence="6">6.3.2.12</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Folylpoly-gamma-glutamate synthetase-dihydrofolate synthetase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">Folylpolyglutamate synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="primary">folC</name>
        <name evidence="15" type="ordered locus">Rmet_2463</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Functions in two distinct reactions of the de novo folate biosynthetic pathway. Catalyzes the addition of a glutamate residue to dihydropteroate (7,8-dihydropteroate or H2Pte) to form dihydrofolate (7,8-dihydrofolate monoglutamate or H2Pte-Glu). Also catalyzes successive additions of L-glutamate to tetrahydrofolate or 10-formyltetrahydrofolate or 5,10-methylenetetrahydrofolate, leading to folylpolyglutamate derivatives.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>(6R)-5,10-methylenetetrahydrofolyl-(gamma-L-Glu)(n) + ATP + L-glutamate = (6R)-5,10-methylenetetrahydrofolyl-(gamma-L-Glu)(n+1) + ADP + H(+) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:51912"/>
            <dbReference type="Rhea" id="RHEA-COMP:13257"/>
            <dbReference type="Rhea" id="RHEA-COMP:13258"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:136572"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.2.17"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>10-formyltetrahydrofolyl-(gamma-L-Glu)(n) + ATP + L-glutamate = 10-formyltetrahydrofolyl-(gamma-L-Glu)(n+1) + ADP + H(+) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:51904"/>
            <dbReference type="Rhea" id="RHEA-COMP:13088"/>
            <dbReference type="Rhea" id="RHEA-COMP:14300"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:134413"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.2.17"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>7,8-dihydropteroate + ATP + L-glutamate = 7,8-dihydrofolate + ADP + H(+) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:23584"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17839"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57451"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.2.12"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="5">Cofactor biosynthesis; tetrahydrofolate biosynthesis; 7,8-dihydrofolate from 2-amino-4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine diphosphate and 4-aminobenzoate: step 2/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the folylpolyglutamate synthase family.</text>
    </comment>
    <dbReference type="EC" id="6.3.2.12" evidence="6"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09340.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517053.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2463"/>
    <dbReference type="EnsemblBacteria" id="ABF09340">
        <property type="protein sequence ID" value="ABF09340"/>
        <property type="gene ID" value="Rmet_2463"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2463"/>
    <dbReference type="eggNOG" id="COG0285">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015869_1_0_4"/>
    <dbReference type="OMA" id="YFEMGTL"/>
    <dbReference type="OrthoDB" id="1232874at2"/>
    <dbReference type="UniPathway" id="UPA00077">
        <property type="reaction ID" value="UER00157"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008841">
        <property type="term" value="F:dihydrofolate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004326">
        <property type="term" value="F:tetrahydrofolylpolyglutamate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046656">
        <property type="term" value="P:folic acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046654">
        <property type="term" value="P:tetrahydrofolate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1190.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001645">
        <property type="entry name" value="Folylpolyglutamate_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018109">
        <property type="entry name" value="Folylpolyglutamate_synth_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036565">
        <property type="entry name" value="Mur-like_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036615">
        <property type="entry name" value="Mur_ligase_C_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013221">
        <property type="entry name" value="Mur_ligase_cen"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11136">
        <property type="entry name" value="PTHR11136"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08245">
        <property type="entry name" value="Mur_ligase_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001563">
        <property type="entry name" value="Folylpolyglu_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53244">
        <property type="entry name" value="SSF53244"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53623">
        <property type="entry name" value="SSF53623"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01499">
        <property type="entry name" value="folC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01011">
        <property type="entry name" value="FOLYLPOLYGLU_SYNT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0289">Folate biosynthesis</keyword>
    <keyword evidence="13 15" id="KW-0436">Ligase</keyword>
    <keyword evidence="9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Mur_ligase_M" evidence="14">
        <location>
            <begin position="47"/>
            <end position="269"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000058"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00000104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00000809"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00002714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004799"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00013023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00019357"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00030048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00032510"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRNR" id="PIRNR001563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF08245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="ABF09340.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="436" mass="47674" checksum="59A73FAE77D9F0DB" modified="2006-05-30" version="1">MPIFHNLPDWLAHLETAHPVGIDMGLSRITRVKEALGLRIDAVVFTVGGTNGKGSTCAILERILLEAGYKVGLHTSPHLIQFNERARLNGEQATDAQLLPHFEAVERARTSFADPVSLTYFEFTTLAIIHMFAEAGLDAIILEVGLGGRLDAVNVIDTDCAIITSVDIDHTQYLGDTREKIGYEKAGIFRPHVPAIVGDPLPPQSVIDHAEAIGAELWLVGRDFRHQAAQGQERQQWDWASRDRKLNGLGYPALRGANQLLNASAALAALQAMRSRLPVSAQEVRNGLAFVELPGRFQVLPGRPAVILDVAHNPHAAATLGQNLENMGFFRYTYAVFGAMHDKDIAGVLQHVADKVDHWCLCDLPTDRAATAAELREKLEATGFREGPDNTVACFSSPELAYRDAVERATENDRILVFGSFYTVAGVMAYRATQAN</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-01-15" modified="2022-08-03" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LKK7</accession>
    <name>MOBA_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Molybdenum cofactor guanylyltransferase</fullName>
            <shortName evidence="1">MoCo guanylyltransferase</shortName>
            <ecNumber evidence="1">2.7.7.77</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">GTP:molybdopterin guanylyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Mo-MPT guanylyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Molybdopterin guanylyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Molybdopterin-guanine dinucleotide synthase</fullName>
            <shortName evidence="1">MGD synthase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">mobA</name>
        <name type="ordered locus">Rmet_2442</name>
    </gene>
    <organism>
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Transfers a GMP moiety from GTP to Mo-molybdopterin (Mo-MPT) cofactor (Moco or molybdenum cofactor) to form Mo-molybdopterin guanine dinucleotide (Mo-MGD) cofactor.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>GTP + H(+) + Mo-molybdopterin = diphosphate + Mo-molybdopterin guanine dinucleotide</text>
            <dbReference type="Rhea" id="RHEA:34243"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:71302"/>
            <dbReference type="ChEBI" id="CHEBI:71310"/>
            <dbReference type="EC" id="2.7.7.77"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="1">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="1">The N-terminal domain determines nucleotide recognition and specific binding, while the C-terminal domain determines the specific binding to the target protein.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the MobA family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.77" evidence="1"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09319.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517036.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q1LKK7"/>
    <dbReference type="SMR" id="Q1LKK7"/>
    <dbReference type="STRING" id="266264.Rmet_2442"/>
    <dbReference type="EnsemblBacteria" id="ABF09319">
        <property type="protein sequence ID" value="ABF09319"/>
        <property type="gene ID" value="Rmet_2442"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2442"/>
    <dbReference type="eggNOG" id="COG0746">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_055597_5_1_4"/>
    <dbReference type="OMA" id="IDFWYAK"/>
    <dbReference type="OrthoDB" id="2019898at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061603">
        <property type="term" value="F:molybdenum cofactor guanylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006777">
        <property type="term" value="P:Mo-molybdopterin cofactor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02503">
        <property type="entry name" value="MobA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00316">
        <property type="entry name" value="MobA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025877">
        <property type="entry name" value="MobA-like_NTP_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013482">
        <property type="entry name" value="Molybde_CF_guanTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12804">
        <property type="entry name" value="NTP_transf_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="SSF53448"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02665">
        <property type="entry name" value="molyb_mobA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0342">GTP-binding</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0501">Molybdenum cofactor biosynthesis</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_1000019139" description="Molybdenum cofactor guanylyltransferase">
        <location>
            <begin position="1"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="12"/>
            <end position="14"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="25"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="53"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="71"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="101"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="101"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00316"/>
        </source>
    </evidence>
    <sequence length="203" mass="21589" checksum="D13D1900A37F3FA6" modified="2006-05-30" version="1">MISRDDITGLILAGGRGSRMGGTDKGLQPLRGVPMAMHTLMRLSAQTGAVLINANRNLAAYESFGVPVVTDSVPDFAGPLAGMLAGLEQCQTGWMVTAPCDSPFLPTDLVQRLAQAIETEDAELAIPVTIDADGRRQTQPVFCLMPASAIDSLVAYLNGGGRKIETWAASHRLVEVPFDDAAAFANINTLDELHMHESEKRAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LKM5</accession>
    <name>Q1LKM5_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Anti-sigma factor, negative regulator of sigma24 activity</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">rseA</name>
        <name evidence="2" type="ordered locus">Rmet_2424</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09301.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517020.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2424"/>
    <dbReference type="EnsemblBacteria" id="ABF09301">
        <property type="protein sequence ID" value="ABF09301"/>
        <property type="gene ID" value="Rmet_2424"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2424"/>
    <dbReference type="eggNOG" id="COG3073">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_081225_0_0_4"/>
    <dbReference type="OMA" id="WVVVPQL"/>
    <dbReference type="OrthoDB" id="1628369at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016989">
        <property type="term" value="F:sigma factor antagonist activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd16328">
        <property type="entry name" value="RseA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005572">
        <property type="entry name" value="Anti-sigma_E_RseA_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036147">
        <property type="entry name" value="Anti-sigma_E_RseA_N_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03872">
        <property type="entry name" value="RseA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89069">
        <property type="entry name" value="SSF89069"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RseA_N" evidence="1">
        <location>
            <begin position="13"/>
            <end position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03872"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF09301.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="218" mass="22755" checksum="2BA55FBCF93A248D" modified="2006-05-30" version="1">MGQAHKQSVHVMEAAEQISALMDGELAPHDVDAALGLAKDDAGMADWSMYQLIGDAMRSEELTHAGSTEAFLSRFSAQLEAEPHVLVPAVAQAGRHRLLVRPSWVRRVVPGTAIAAAVAAVSWVVVPQMRGPGGVGAPDAVVASVEQPAGATVKAGVAAPAAAGSIMAVSADSAQMIRDPRLDEYLRAHRTSVATDAFVPTMRTVVNGVNFSQDNSQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LKS4</accession>
    <name>Q1LKS4_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Cytochrome-c oxidase</fullName>
            <ecNumber evidence="3">7.1.1.9</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">ccoN</name>
        <name evidence="8" type="ordered locus">Rmet_2375</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>4 Fe(II)-[cytochrome c] + 8 H(+)(in) + O2 = 4 Fe(III)-[cytochrome c] + 4 H(+)(out) + 2 H2O</text>
            <dbReference type="Rhea" id="RHEA:11436"/>
            <dbReference type="Rhea" id="RHEA-COMP:10350"/>
            <dbReference type="Rhea" id="RHEA-COMP:14399"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
            <dbReference type="EC" id="7.1.1.9"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>Cu(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29036"/>
        </cofactor>
        <text evidence="5">Binds 1 copper ion per subunit, denoted as copper B.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
        <text evidence="5">Binds 2 heme groups per subunit, denoted as high- and low-spin.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Energy metabolism; oxidative phosphorylation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the heme-copper respiratory oxidase family.</text>
    </comment>
    <dbReference type="EC" id="7.1.1.9" evidence="3"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09252.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_003149798.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2375"/>
    <dbReference type="EnsemblBacteria" id="ABF09252">
        <property type="protein sequence ID" value="ABF09252"/>
        <property type="gene ID" value="Rmet_2375"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2375"/>
    <dbReference type="eggNOG" id="COG3278">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017702_3_4_4"/>
    <dbReference type="OrthoDB" id="665754at2"/>
    <dbReference type="UniPathway" id="UPA00705"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045278">
        <property type="term" value="C:plasma membrane respiratory chain complex IV"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004129">
        <property type="term" value="F:cytochrome-c oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006119">
        <property type="term" value="P:oxidative phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.210.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023616">
        <property type="entry name" value="Cyt_c_oxase-like_su1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036927">
        <property type="entry name" value="Cyt_c_oxase-like_su1_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000883">
        <property type="entry name" value="Cyt_C_Oxase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004677">
        <property type="entry name" value="Cyt_c_oxidase_cbb3_su1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10422">
        <property type="entry name" value="PTHR10422"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10422:SF29">
        <property type="entry name" value="PTHR10422:SF29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00115">
        <property type="entry name" value="COX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81442">
        <property type="entry name" value="SSF81442"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50855">
        <property type="entry name" value="COX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0349">Heme</keyword>
    <keyword evidence="5" id="KW-0408">Iron</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="6"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="80"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="130"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="160"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="193"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="228"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="262"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="296"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="324"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="367"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="404"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="439"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="490"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="domain" description="COX1" evidence="7">
        <location>
            <begin position="238"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="265"/>
        </location>
        <ligand>
            <name>Cu cation</name>
            <dbReference type="ChEBI" id="CHEBI:23378"/>
            <label>B</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="315"/>
        </location>
        <ligand>
            <name>Cu cation</name>
            <dbReference type="ChEBI" id="CHEBI:23378"/>
            <label>B</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="316"/>
        </location>
        <ligand>
            <name>Cu cation</name>
            <dbReference type="ChEBI" id="CHEBI:23378"/>
            <label>B</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009578"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00029368"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR604677-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50855"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ABF09252.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="518" mass="56779" checksum="17B51F93D11CC56A" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="56" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LKX6</accession>
    <name>Q1LKX6_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Conserved plasmid protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">cggAII</name>
        <name evidence="1" type="ordered locus">Rmet_2323</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09200.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_003116822.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2323"/>
    <dbReference type="EnsemblBacteria" id="ABF09200">
        <property type="protein sequence ID" value="ABF09200"/>
        <property type="gene ID" value="Rmet_2323"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2323"/>
    <dbReference type="eggNOG" id="ENOG5030MHX">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_143373_1_0_4"/>
    <dbReference type="OMA" id="EHAQRGC"/>
    <dbReference type="OrthoDB" id="1639559at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF09200.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="113" mass="12630" checksum="8372721BC360CE52" modified="2006-05-30" version="1">MSTIACDTPRSTLDEPAWRDLCKAAAAHAQRGCGLSYDHYVTLFSSAIDAQANRLPDEQRAQALEIAAQEWDYATPAERQETQDWNAEHGYCSHGIEFGYCPAGCDRDDDDWD</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="64" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LL08</accession>
    <name>Q1LL08_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">DNA-binding transcriptional activator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">alpA</name>
        <name evidence="1" type="ordered locus">Rmet_2289</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09168.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_003090093.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2289"/>
    <dbReference type="EnsemblBacteria" id="ABF09168">
        <property type="protein sequence ID" value="ABF09168"/>
        <property type="gene ID" value="Rmet_2289"/>
    </dbReference>
    <dbReference type="GeneID" id="56409798"/>
    <dbReference type="KEGG" id="rme:Rmet_2289"/>
    <dbReference type="eggNOG" id="COG3311">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_140176_15_1_4"/>
    <dbReference type="OMA" id="RHIMRRD"/>
    <dbReference type="OrthoDB" id="2076478at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010260">
        <property type="entry name" value="AlpA"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05930">
        <property type="entry name" value="Phage_AlpA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF09168.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="70" mass="8282" checksum="CEEF2825C016E230" modified="2006-05-30" version="1">MSQTPVLPPNERRILRLDEVEAKSGFKRAHIYNLMKKRQFPQALRLGVRAVGWDSIEIDQWIAERLNHRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LL18</accession>
    <name>Q1LL18_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Biopolymer transport protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">exbD1</name>
        <name evidence="8" type="ordered locus">Rmet_2279</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cell membrane</location>
            <topology evidence="6">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the ExbD/TolR family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09158.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516983.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2279"/>
    <dbReference type="EnsemblBacteria" id="ABF09158">
        <property type="protein sequence ID" value="ABF09158"/>
        <property type="gene ID" value="Rmet_2279"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2279"/>
    <dbReference type="eggNOG" id="COG0848">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_085305_1_1_4"/>
    <dbReference type="OMA" id="INHAVKI"/>
    <dbReference type="OrthoDB" id="1940365at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003400">
        <property type="entry name" value="ExbD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30558">
        <property type="entry name" value="PTHR30558"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02472">
        <property type="entry name" value="ExbD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0653">Protein transport</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="20"/>
            <end position="40"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ABF09158.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="137" mass="15207" checksum="BF9A2A3BCB440A2F" modified="2006-05-30" version="1">MAFGTLDGDDDEVMSEINMTPLVDVMLVLLIIFIITIPVINHAVKIDLPRATNQPNDAKPQSINVSIDAQGKVFWNQQEVDDATLDQRISEAALQQPQPELHLRADREVRYERVAQVMAAAQHGGLGKIGFITEPKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LLA0</accession>
    <name>Q1LLA0_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">4-hydroxybenzoyl CoA thioesterase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_2197</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09076.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516904.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2197"/>
    <dbReference type="EnsemblBacteria" id="ABF09076">
        <property type="protein sequence ID" value="ABF09076"/>
        <property type="gene ID" value="Rmet_2197"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2197"/>
    <dbReference type="eggNOG" id="COG0824">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_101141_5_2_4"/>
    <dbReference type="OMA" id="SHCDPAG"/>
    <dbReference type="OrthoDB" id="1786865at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029069">
        <property type="entry name" value="HotDog_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006683">
        <property type="entry name" value="Thioestr_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03061">
        <property type="entry name" value="4HBT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54637">
        <property type="entry name" value="SSF54637"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="4HBT" evidence="1">
        <location>
            <begin position="20"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF09076.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="139" mass="15770" checksum="539B124C6900E834" modified="2006-05-30" version="1">MSDVFTNQVRVRFKHCDTAGIVFYPRYFEMLNDFIEDWFAEALDWPFDAMHLTGHAGVPTAELQCRFEAPSRLGELLTRELRVSHIGRSSFSVEVRLAGPDGGTRMHISQRLVCVDTRTMTAQALPDPVRHAMGRFLAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LLH1</accession>
    <name>Q1LLH1_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Transcriptional regulator, LysR-family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_2126</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the LysR transcriptional regulatory family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09005.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516839.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2126"/>
    <dbReference type="EnsemblBacteria" id="ABF09005">
        <property type="protein sequence ID" value="ABF09005"/>
        <property type="gene ID" value="Rmet_2126"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2126"/>
    <dbReference type="eggNOG" id="COG0583">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039613_6_0_4"/>
    <dbReference type="OMA" id="VRVCANT"/>
    <dbReference type="OrthoDB" id="990666at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005119">
        <property type="entry name" value="LysR_subst-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000847">
        <property type="entry name" value="Tscrpt_reg_HTH_LysR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00126">
        <property type="entry name" value="HTH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03466">
        <property type="entry name" value="LysR_substrate"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50931">
        <property type="entry name" value="HTH_LYSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH lysR-type" evidence="2">
        <location>
            <begin position="1"/>
            <end position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF09005.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="301" mass="32690" checksum="687BBFF4FF231FF1" modified="2006-05-30" version="1">MNLARFDLVTLGLFVAVARQGSISAGARQSHLAVAAASKRISDLETAVGAPLFFRHAAGVQLTEAGQACFHHALTILQDVERMAGVMSDYASGVRGQVRVCANTSAITQFLPDDLAQFMRQHPTIRIELEEHNSSDIVAAVMENRADVGIFADRTPCAGLVTLEYRQDELVIITPPGHPLAQNGPVHYADALEYDFVSLPQVTSLAARLLEESSRLERQFRLRIQVRSFDAMCRMVMAGLGVGVLPRIAAEPHVKSMGLSLVGLRDAWARRSLLIGMRDPAGLTVSARLLISHLCGDRAPF</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="95" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LLH7</accession>
    <name>Q1LLH7_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">ABC-type transporter, permease component: TauT family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Rmet_2120</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cell membrane</location>
            <topology evidence="4">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the binding-protein-dependent transport system permease family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08999.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516833.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2120"/>
    <dbReference type="EnsemblBacteria" id="ABF08999">
        <property type="protein sequence ID" value="ABF08999"/>
        <property type="gene ID" value="Rmet_2120"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2120"/>
    <dbReference type="eggNOG" id="COG0600">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046113_2_2_4"/>
    <dbReference type="OMA" id="KVWGRIW"/>
    <dbReference type="OrthoDB" id="916788at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="SSF161098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2 4" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="12"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="67"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="110"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="181"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="224"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="5">
        <location>
            <begin position="63"/>
            <end position="243"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABF08999.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="263" mass="29034" checksum="B39962BF4D750645" modified="2006-05-30" version="1">MAFRPDSKGMLRVWQLLLLAVVLIVWHMATRSQQVAFFFGEPLIVAQRIWSWFIVERDIYLHLGVTLLETVLAFGIGTVSGLGVGLWLALSPFTSAVLDPYVKAANSMPRVILAPIFAVWFGLGIWSKVALAVTLVFFIVFFNVYQGVKEVSPVVLANARMLGANKRQLLRHVYLPSATSWVFSSLHTSVGLAFVGAVVGEYLGSARGVGYLILQAEGTFDINTVFAGIVVLTAFALVLDWMVTVGEKRLMRWQPKSGETEKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LLI3</accession>
    <name>Q1LLI3_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">3'-5' exonuclease</fullName>
            <ecNumber evidence="2">3.1.11.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_2114</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EC" id="3.1.11.-" evidence="2"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08993.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516827.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2114"/>
    <dbReference type="EnsemblBacteria" id="ABF08993">
        <property type="protein sequence ID" value="ABF08993"/>
        <property type="gene ID" value="Rmet_2114"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2114"/>
    <dbReference type="eggNOG" id="COG3298">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069554_1_0_4"/>
    <dbReference type="OMA" id="VLHYRGL"/>
    <dbReference type="OrthoDB" id="1985371at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004527">
        <property type="term" value="F:exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019288">
        <property type="entry name" value="3'-5'_exonuclease_PolB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10108">
        <property type="entry name" value="DNA_pol_B_exo2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="SSF53098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0540">Nuclease</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA_pol_B_exo2" evidence="1">
        <location>
            <begin position="47"/>
            <end position="264"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF10108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF08993.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="278" mass="31867" checksum="DA3471A994ECF513" modified="2006-05-30" version="1">MTPVLVFDIETIPDVDGLRRLHDHPASMSDAEVAEHAFAARREKTGSDFLPHYLQRVAAISCVLRRTQRDGSAVFHVGSLGTLEDGEATLIQKFYELIARYSPQLVSWNGGGFDLPVLHYRGLVHGVSAPRYWEMGEGRDDDSRDFKWNNYISRYHMRHLDLMDLLAMYQPRASAPLDDLAKLCGFPGKMGMDGSKVWEAYQAGKLKEIRDYCETDVVNTYLMYCRFQLMRGGLTPREFDMEVALTQETLAELHGEQWMEYLRAFRLQPMSPESEDDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="107" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LLK7</accession>
    <name>Q1LLK7_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">NAD-dependent protein deacylase</fullName>
            <ecNumber evidence="3">2.3.1.286</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">Regulatory protein SIR2 homolog</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5" type="primary">npdA</name>
        <name evidence="3" type="synonym">cobB</name>
        <name evidence="5" type="ordered locus">Rmet_2090</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">NAD-dependent lysine deacetylase and desuccinylase that specifically removes acetyl and succinyl groups on target proteins. Modulates the activities of several proteins which are inactive in their acylated form.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>H2O + N(6)-acetyl-L-lysyl-[protein] + NAD(+) = 2''-O-acetyl-ADP-D-ribose + L-lysyl-[protein] + nicotinamide</text>
            <dbReference type="Rhea" id="RHEA:43636"/>
            <dbReference type="Rhea" id="RHEA-COMP:9752"/>
            <dbReference type="Rhea" id="RHEA-COMP:10731"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:17154"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:61930"/>
            <dbReference type="ChEBI" id="CHEBI:83767"/>
            <dbReference type="EC" id="2.3.1.286"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>H2O + N(6)-succinyl-L-lysyl-[protein] + NAD(+) = 2''-O-succinyl-ADP-D-ribose + L-lysyl-[protein] + nicotinamide</text>
            <dbReference type="Rhea" id="RHEA:47668"/>
            <dbReference type="Rhea" id="RHEA-COMP:9752"/>
            <dbReference type="Rhea" id="RHEA-COMP:11877"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:17154"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:87830"/>
            <dbReference type="ChEBI" id="CHEBI:87832"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="3">2 residues (Tyr-69 and Arg-72) present in a large hydrophobic pocket are probably involved in substrate specificity. They are important for desuccinylation activity, but dispensable for deacetylation activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the sirtuin family. Class III subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.286" evidence="3"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08969.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516803.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
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    <dbReference type="EnsemblBacteria" id="ABF08969">
        <property type="protein sequence ID" value="ABF08969"/>
        <property type="gene ID" value="Rmet_2090"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2090"/>
    <dbReference type="eggNOG" id="COG0846">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023643_3_1_4"/>
    <dbReference type="OMA" id="EQCKKCR"/>
    <dbReference type="OrthoDB" id="1264438at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070403">
        <property type="term" value="F:NAD+ binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034979">
        <property type="term" value="F:NAD-dependent protein deacetylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036054">
        <property type="term" value="F:protein-malonyllysine demalonylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036055">
        <property type="term" value="F:protein-succinyllysine desuccinylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006476">
        <property type="term" value="P:protein deacetylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01412">
        <property type="entry name" value="SIRT5_Af1_CobB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1600.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01121">
        <property type="entry name" value="Sirtuin_ClassIII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029035">
        <property type="entry name" value="DHS-like_NAD/FAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003000">
        <property type="entry name" value="Sirtuin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026591">
        <property type="entry name" value="Sirtuin_cat_small_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027546">
        <property type="entry name" value="Sirtuin_class_III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026590">
        <property type="entry name" value="Ssirtuin_cat_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02146">
        <property type="entry name" value="SIR2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52467">
        <property type="entry name" value="SSF52467"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50305">
        <property type="entry name" value="SIRTUIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 3" id="KW-0520">NAD</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Deacetylase sirtuin-type" evidence="4">
        <location>
            <begin position="7"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="3">
        <location>
            <position position="120"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="69"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="72"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="102"/>
            <end position="105"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="188"/>
            <end position="190"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="214"/>
            <end position="216"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="232"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABF08969.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="244" mass="26380" checksum="AC887B99D8F544E5" modified="2006-05-30" version="1">MDSLALEQARGWVAEARQIFVLTGAGISAESGVPTFRDALTGLWERFDPEELATEEAYRRQPALVWQWYQHRRELVAAARPNPAHHALAVLARLKSMTLVTQNIDGLHQKAGSEQVVELHGNLFANKWLDGCGRCDTVPPIPGDPPRCAICGAMMRPGVVWFGEDLPRVARFRADHAAESCDLCLVVGTSGLVYPAAALPGVAKENGARVIVVNPKPSALDETADIVLPAPAGECLPLLWPQAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="72" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LLN4</accession>
    <name>Q1LLN4_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Rhodanese-related sulfurtransferase</fullName>
            <ecNumber evidence="2">2.8.1.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_2063</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.8.1.-" evidence="2"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08942.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516776.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2063"/>
    <dbReference type="EnsemblBacteria" id="ABF08942">
        <property type="protein sequence ID" value="ABF08942"/>
        <property type="gene ID" value="Rmet_2063"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2063"/>
    <dbReference type="eggNOG" id="COG0607">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_089574_10_1_4"/>
    <dbReference type="OMA" id="KFVGHVP"/>
    <dbReference type="OrthoDB" id="2056793at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.250.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001763">
        <property type="entry name" value="Rhodanese-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036873">
        <property type="entry name" value="Rhodanese-like_dom_sf"/>
    </dbReference>
    <dbReference type="SMART" id="SM00450">
        <property type="entry name" value="RHOD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52821">
        <property type="entry name" value="SSF52821"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50206">
        <property type="entry name" value="RHODANESE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Rhodanese" evidence="1">
        <location>
            <begin position="37"/>
            <end position="140"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF08942.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="154" mass="17127" checksum="18508A9FD688A19D" modified="2006-05-30" version="1">MTTRDELLQAAQTRKQQDELAYYGALTPQEAYALLQAEPKAVLVDVRTQAELDWVGGVDLPDEQFAHIEWMSYPGGAQNANFVAELKARVPADAPVLFLCRSAARSKHAARVATENGYLLAMDVLEGFEGNRDDHHHRKTVEGWCVRGLPWHGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="56" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LLQ8</accession>
    <name>Q1LLQ8_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_2039</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08918.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516752.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2039"/>
    <dbReference type="EnsemblBacteria" id="ABF08918">
        <property type="protein sequence ID" value="ABF08918"/>
        <property type="gene ID" value="Rmet_2039"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2039"/>
    <dbReference type="eggNOG" id="COG3198">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_184317_1_0_4"/>
    <dbReference type="OMA" id="PWWREPW"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="21"/>
            <end position="44"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF08918.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="78" mass="8531" checksum="239A008D1363EC8D" modified="2006-05-30" version="1">MAANKESAMTSQQSKPWYREPWPWLLMSGPLLAIIGCGVTIWLANTHPDLPVSGVERHGLVVEKTGAEHVTSPAARRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LLY3</accession>
    <name>Q1LLY3_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Molybdopterin molybdenumtransferase</fullName>
            <ecNumber evidence="7">2.10.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">moeA</name>
        <name evidence="9" type="ordered locus">Rmet_1964</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2 7">Catalyzes the insertion of molybdate into adenylated molybdopterin with the concomitant release of AMP.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>adenylyl-molybdopterin + H(+) + molybdate = AMP + H2O + Mo-molybdopterin</text>
            <dbReference type="Rhea" id="RHEA:35047"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:36264"/>
            <dbReference type="ChEBI" id="CHEBI:62727"/>
            <dbReference type="ChEBI" id="CHEBI:71302"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="2.10.1.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="7">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3 7">Cofactor biosynthesis; molybdopterin biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 7">Belongs to the MoeA family.</text>
    </comment>
    <dbReference type="EC" id="2.10.1.1" evidence="7"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08843.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516688.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1964"/>
    <dbReference type="EnsemblBacteria" id="ABF08843">
        <property type="protein sequence ID" value="ABF08843"/>
        <property type="gene ID" value="Rmet_1964"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1964"/>
    <dbReference type="eggNOG" id="COG0303">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010186_7_0_4"/>
    <dbReference type="OMA" id="GKPLMHG"/>
    <dbReference type="OrthoDB" id="651103at2"/>
    <dbReference type="UniPathway" id="UPA00344"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061599">
        <property type="term" value="F:molybdopterin molybdotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006777">
        <property type="term" value="P:Mo-molybdopterin cofactor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032324">
        <property type="term" value="P:molybdopterin cofactor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00887">
        <property type="entry name" value="MoeA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.340.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.980.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036425">
        <property type="entry name" value="MoaB/Mog-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001453">
        <property type="entry name" value="MoaB/Mog_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008284">
        <property type="entry name" value="MoCF_biosynth_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038987">
        <property type="entry name" value="MoeA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005111">
        <property type="entry name" value="MoeA_C_domain_IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036688">
        <property type="entry name" value="MoeA_C_domain_IV_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005110">
        <property type="entry name" value="MoeA_linker/N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036135">
        <property type="entry name" value="MoeA_linker/N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10192">
        <property type="entry name" value="PTHR10192"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00994">
        <property type="entry name" value="MoCF_biosynth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03454">
        <property type="entry name" value="MoeA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03453">
        <property type="entry name" value="MoeA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00852">
        <property type="entry name" value="MoCF_biosynth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53218">
        <property type="entry name" value="SSF53218"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63867">
        <property type="entry name" value="SSF63867"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63882">
        <property type="entry name" value="SSF63882"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01079">
        <property type="entry name" value="MOCF_BIOSYNTHESIS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 7" id="KW-0500">Molybdenum</keyword>
    <keyword evidence="6 7" id="KW-0501">Molybdenum cofactor biosynthesis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="MoCF_biosynth" evidence="8">
        <location>
            <begin position="184"/>
            <end position="326"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002901"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005046"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022505"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU365090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ABF08843.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="411" mass="43398" checksum="10637D76DE27B705" modified="2006-05-30" version="1">MTQTAARPPMLTMAQALDALMAGVRPITDSETVDTLQANGRVLAAGVTSALRVPPADNTSMDGYVMRAADVPAPGTRLPVSQRIPAGHVGTELAPGTAARIFTGGLIPPGADAVVMQEQCQADGDAVIVNHAPASGEWIRRAGEDIEAGSEILPAGTRLSPQALGLAASVGQAKLDVVRRVRVAVFFTGDELAMPGEPLKPGAIYNSNRFTLRGLLENLGCEVTDFGIVPDTLAATRDTLRRAAEGNDLIITSGGVSVGEEDHIRPAVTAEGRLDLWQIAIKPGKPLAFGQVANGTELPAFFLGLPGNPVSSFVTFLLFVRPFILRLQGVADVTPRRLPLRADFDLPRGDRRNEFLRARINDAGGLDLFPNQSSGVLTSTVWGDGLIDNPPNQTIQRGDVVQFIPFASLLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LM98</accession>
    <name>Q1LM98_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative oxidoreductase/alcolhol dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_1849</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08728.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516577.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1849"/>
    <dbReference type="EnsemblBacteria" id="ABF08728">
        <property type="protein sequence ID" value="ABF08728"/>
        <property type="gene ID" value="Rmet_1849"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1849"/>
    <dbReference type="eggNOG" id="COG2130">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026673_29_3_4"/>
    <dbReference type="OMA" id="GKDKCDY"/>
    <dbReference type="OrthoDB" id="474248at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016628">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-CH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013149">
        <property type="entry name" value="ADH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041694">
        <property type="entry name" value="ADH_N_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045010">
        <property type="entry name" value="MDR_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020843">
        <property type="entry name" value="PKS_ER"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43205">
        <property type="entry name" value="PTHR43205"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16884">
        <property type="entry name" value="ADH_N_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00107">
        <property type="entry name" value="ADH_zinc_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00829">
        <property type="entry name" value="PKS_ER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="SSF50129"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PKS_ER" evidence="1">
        <location>
            <begin position="19"/>
            <end position="334"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF08728.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="337" mass="36083" checksum="57568E7BCBADB197" modified="2006-05-30" version="1">MSKQTYKRIVLASRPEAAVTPDNFRIEELPVPELADGQVLVRNHFLSLDPYMRGRMSDSKSYADPQPLNEVMIGGTVGEVIASKNPKWNVGDKVIGMFGWQELGVSDGGMMQKVDTTHIPLSAYLGSVGMPGVTAWYGLNKIIAPKAGETVVVSAASGAVGSVVGQLAKLAGCYVVGVAGGKEKCDYVVNELGFDACVDYKAAKDAKELYAMVKAVTPNGIDGYFENVGGDILDVVLSRLNPFARIAVCGLIAGYDGQDMPIRNPRAILVARAKIEGFIVSEHMELWPQALRELGTYVAQGKIKFRESIAEGLASAPEAFIGLLKGKNFGKQLVKLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LME2</accession>
    <name>Q1LME2_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Rmet_1805</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08684.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516534.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1805"/>
    <dbReference type="EnsemblBacteria" id="ABF08684">
        <property type="protein sequence ID" value="ABF08684"/>
        <property type="gene ID" value="Rmet_1805"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1805"/>
    <dbReference type="eggNOG" id="COG0392">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_052307_0_0_4"/>
    <dbReference type="OMA" id="LMGGEPY"/>
    <dbReference type="OrthoDB" id="1585225at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022791">
        <property type="entry name" value="L-PG_synthase/AglD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03706">
        <property type="entry name" value="LPG_synthase_TM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00374">
        <property type="entry name" value="TIGR00374"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="37"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="112"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="150"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="214"/>
            <end position="239"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABF08684.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="332" mass="35709" checksum="89D7881B1323F1B2" modified="2006-05-30" version="1">MSRAAFLLFSIGAALFVGLLAWQGFDAVTTTLMSAGWGLAVVAAFHLLPLLLDAGAIAVLLDRKTRHGTFCSALRARWTGESVNSLLPAGQIGGPVLMVRYLSHRGARMRDAAAAITVSTTTQALSQMIFALLGILLFGAQGNLSDQRTPIIVVTVILAACVLVFCVLQRRGMFGRVLRMAAKVFGKQNWDGLALRADAVDQAIREMYRDRRKVAISFLLNLAGWIAGTGEVWLILYFLGHPVGWHEALLLESVGQAIRGAAFAIPGSLGAQEGGYLLLAPLVGLPPDAALALSLAKRVRELVLGIPGLIYLHFSERKFQRRRARAALQGTD</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="103" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LMP8</accession>
    <name>Q1LMP8_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Histidine kinase</fullName>
            <ecNumber evidence="2">2.7.13.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Rmet_1697</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.</text>
            <dbReference type="EC" id="2.7.13.3"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.7.13.3" evidence="2"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08578.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516430.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1697"/>
    <dbReference type="EnsemblBacteria" id="ABF08578">
        <property type="protein sequence ID" value="ABF08578"/>
        <property type="gene ID" value="Rmet_1697"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1697"/>
    <dbReference type="eggNOG" id="COG4191">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_876818_0_0_4"/>
    <dbReference type="OMA" id="LWPACLD"/>
    <dbReference type="OrthoDB" id="1755994at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00082">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003661">
        <property type="entry name" value="HisK_dim/P"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036097">
        <property type="entry name" value="HisK_dim/P_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004358">
        <property type="entry name" value="Sig_transdc_His_kin-like_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00512">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00344">
        <property type="entry name" value="BCTRLSENSOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00388">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47384">
        <property type="entry name" value="SSF47384"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="SSF55874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193685" description="Histidine kinase" evidence="5">
        <location>
            <begin position="19"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="6">
        <location>
            <begin position="63"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="295"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="299"/>
            <end position="317"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABF08578.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="317" mass="33895" checksum="A52DC1EF9E403A68" modified="2006-05-30" version="1">MRWLSFLLVGISSGVAFAAAIVPSFGRRRSHSGGTTESVQSSNHAREIAHLSPAPGMNELAGSIAHELNQPLTAITSNAQAAREYLASGPCDPAEVREILDDIVADASRASEIVRRVRALILGEAPSAIHFNVGEVVDQAIALVDANARARSVLVSREVANSLPRAVGDPMEIQLVLLNLLSNALDAVESVRPHGERSASILAQERNGMVVVSVRDNGCGVEPGQLERIFQPFYSSKHKGMGLGLSFSRALVERNGGRLSAIRNSNTGMTFELSLRTEDAAQDLRGSVLPLAPRGIHPLHHGGKNDERNAANGMYRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LN16</accession>
    <name>Q1LN16_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Betaine-aldehyde dehydrogenase</fullName>
            <ecNumber evidence="5">1.2.1.8</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">betB</name>
        <name evidence="5" type="ordered locus">Rmet_1577</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the aldehyde dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.2.1.8" evidence="5"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08460.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516312.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1577"/>
    <dbReference type="EnsemblBacteria" id="ABF08460">
        <property type="protein sequence ID" value="ABF08460"/>
        <property type="gene ID" value="Rmet_1577"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1577"/>
    <dbReference type="eggNOG" id="COG1012">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005391_0_2_4"/>
    <dbReference type="OMA" id="DINGGPF"/>
    <dbReference type="OrthoDB" id="618655at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008802">
        <property type="term" value="F:betaine-aldehyde dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.309.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.605.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016161">
        <property type="entry name" value="Ald_DH/histidinol_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016163">
        <property type="entry name" value="Ald_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029510">
        <property type="entry name" value="Ald_DH_CS_GLU"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016162">
        <property type="entry name" value="Ald_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015590">
        <property type="entry name" value="Aldehyde_DH_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00171">
        <property type="entry name" value="Aldedh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53720">
        <property type="entry name" value="SSF53720"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00687">
        <property type="entry name" value="ALDEHYDE_DEHYDR_GLU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 3 5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aldedh" evidence="4">
        <location>
            <begin position="13"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="active site" evidence="2">
        <location>
            <position position="245"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABF08460.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="476" mass="50255" checksum="5BD49033C4401888" modified="2006-05-30" version="1">MQQRDKLFINGSWVTPHGTGFIDVIHSATEDVMGKIPEGDARDAENAIAAARAAFEAWSATPPSVRADYIRKIAEGLKARCEELAQMIAGEVGMPIKLARAIQVGGPVYNWGQAAALLDQFAFEEQVGNSLVVREPVGVVAAITPWNYPLNQITLKVAPALAAGCTVVLKPSEVAPLNAFVLAEVIEAAGLPPGVFNLVTGYGPVVGEVLASHPEVDMVSFTGSTRAGKRVSELAAQSVKRVALELGGKSASVILDDADLPTAVKGTIGACYLNSGQTCSAHTRMLVPRSRYEEVKAIAAKVVEGFTVGDPLADTTKLGPLISAVQKERVTGYIRRGLDEGAELVAGGPDTPAGLDRGFFVKPTVLGNVDPKATVAQEEIFGPVLSVICYDTEDEAIRIANDSIYGLGGGVWSGDESRAIRVARRIRTGQVDINGGPFNMNAPFGGFKQSGNGREGGKFGLEEFLEYKSLQLKKPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LNC5</accession>
    <name>Q1LNC5_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Phage-related protein (Modular protein)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_1468</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08351.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1468"/>
    <dbReference type="EnsemblBacteria" id="ABF08351">
        <property type="protein sequence ID" value="ABF08351"/>
        <property type="gene ID" value="Rmet_1468"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1468"/>
    <dbReference type="eggNOG" id="COG3311">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_140176_2_0_4"/>
    <dbReference type="OMA" id="TIRKHEL"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010260">
        <property type="entry name" value="AlpA"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05930">
        <property type="entry name" value="Phage_AlpA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="107"/>
            <end position="136"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF08351.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="136" mass="15528" checksum="C212A6BED1BEB13F" modified="2006-05-30" version="1">MSPCSVLMESEVEHLSQTTNAAGIDNDIGRSLPLPGTEAYEALPQFPKRSALPFRRTIRRQELRQIVPLAETTIYEMERRGEFPRRFNLTPRCVVWDLAEVEAWVEERKQAPRGGTSKPDVHLRKTRPVRAGSSEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LNM3</accession>
    <name>Q1LNM3_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Alkanesulfonate transporter subunit periplasmic-binding component of ABC superfamily</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">ssuA</name>
        <name evidence="5" type="ordered locus">Rmet_1370</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Periplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the bacterial solute-binding protein SsuA/TauA family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08253.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516132.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1370"/>
    <dbReference type="EnsemblBacteria" id="ABF08253">
        <property type="protein sequence ID" value="ABF08253"/>
        <property type="gene ID" value="Rmet_1370"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1370"/>
    <dbReference type="eggNOG" id="COG0715">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028871_2_0_4"/>
    <dbReference type="OMA" id="YQLKLIP"/>
    <dbReference type="OrthoDB" id="785019at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042597">
        <property type="term" value="C:periplasmic space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042626">
        <property type="term" value="F:ATPase-coupled transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010067">
        <property type="entry name" value="ABC_SsuA_sub-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001638">
        <property type="entry name" value="Solute-binding_3/MltF_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015168">
        <property type="entry name" value="SsuA/THI5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09084">
        <property type="entry name" value="NMT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00062">
        <property type="entry name" value="PBPb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01728">
        <property type="entry name" value="SsuA_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="domain" description="PBPb" evidence="4">
        <location>
            <begin position="49"/>
            <end position="270"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010742"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00062"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABF08253.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="345" mass="37187" checksum="FFC20FD9348BAA7B" modified="2006-05-30" version="1">MSSPIRISLFHNSLRRRLLTGVLVVATGLSYVLFAGPAKAQSQTVNPKLLRIGYQKYGTLTLLKARGTLEKRLAPQGITVKWTEFPAGPQLLEGLNVGAIDFGTVGEAPPIFAQAAGAQLAYIGNEPPAPTAEAIVVPKNSTIKSVADLRGKRVALNKGSNVHYLLVRQLEKAGVAYGEIQPVYLPPADARAAFERGAVDAWVIWDPFLAAAQKQLDARVLVDGRGADGKNVVSNHQFYLASRPYAQARPEVVTAILDELAQLGQWAEKHPKDAAAVLVKETSLDPGVLDLAVSRFSYGAKPVSPEVLAEQQRIADVFQTLKLIPKPIRVADAAWQAPTTQQAQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LNR0</accession>
    <name>Q1LNR0_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">3-oxoacyl-(Acyl-carrier-protein) reductase (Protein belongs to CMGI-2)</fullName>
            <ecNumber evidence="3">1.1.1.100</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">phaB2</name>
        <name evidence="3" type="ordered locus">Rmet_1333</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.100" evidence="3"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08216.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516098.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1333"/>
    <dbReference type="EnsemblBacteria" id="ABF08216">
        <property type="protein sequence ID" value="ABF08216"/>
        <property type="gene ID" value="Rmet_1333"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1333"/>
    <dbReference type="eggNOG" id="COG1028">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010194_1_3_4"/>
    <dbReference type="OMA" id="KLDKAGW"/>
    <dbReference type="OrthoDB" id="1601931at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004316">
        <property type="term" value="F:3-oxoacyl-[acyl-carrier-protein] reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018454">
        <property type="term" value="F:acetoacetyl-CoA reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042619">
        <property type="term" value="P:poly-hydroxybutyrate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011283">
        <property type="entry name" value="Acetoacetyl-CoA_reductase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01829">
        <property type="entry name" value="AcAcCoA_reduct"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00061">
        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF08216.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="248" mass="26437" checksum="F9EB83B295E3FEF6" modified="2006-05-30" version="1">MNTTQRTALVTGGNRGLGAAIARALHDAGHRVIVTHTPGNTTIGQWQQAQATQGYSFAAYGVDVSDYESTQELARRIHADGHRIDILVNNAGITRDATLRKLDKAGWDAVLRTNLDSMFNVTKPFIDPMAERGWGRIVNISSINGSKGQFGQTNYSAAKAGVHGFTKALAQEVARKGVTVNTVSPGYLATEMVMAVREDMRQKIIDAIPVGRLGQPAEIAALVAFIASEAAAFMTGSNVAMNGGQHMS</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="70" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LNY0</accession>
    <name>Q1LNY0_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Putative secreted protein (Protein belongs to CMGI-2)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_1261</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08146.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516033.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1261"/>
    <dbReference type="EnsemblBacteria" id="ABF08146">
        <property type="protein sequence ID" value="ABF08146"/>
        <property type="gene ID" value="Rmet_1261"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1261"/>
    <dbReference type="eggNOG" id="COG3528">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_055418_0_0_4"/>
    <dbReference type="OMA" id="FIQGNSP"/>
    <dbReference type="OrthoDB" id="1111569at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.128.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018707">
        <property type="entry name" value="LpxR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037107">
        <property type="entry name" value="Put_OMP_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09982">
        <property type="entry name" value="DUF2219"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF08146.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="375" mass="41444" checksum="579131F66D087B47" modified="2006-05-30" version="1">MLIAGALFSPISTFGSEVSLSTNPSSCSPEQSLRWRGGTLRLENDLFTGSDRNYTNGVALTAVSRDLQGGLRPECLPQPIGLYARFIGWADPGFWHEAGAHSSSQNLVVRFGQAMYTPENKTRTDVIPDDRPYAGLLYLGLAWNRRIHPQAASYEMLDVRELTLGVVGPWSLAEQSQDLVHRARGIERFRGWNNQLRNEPAFQMAMERKFKPYTEGAVRPGWGSDVIGSYALRVGNIETAASTGVEFRAGWNIPNDFGSYPIRPGAENRPPSGVADLRTTTPQSVLAPKPGAHVFLSLEGKAVAWDFSLDGNMFRHSHHVSRRPWVAQAALGISSQWIVAGRGVRLAVMRVWRTREFDQQAGHHAFGSIALSLEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LP01</accession>
    <name>Q1LP01_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Tyrosine-based site-specific recombinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">ritA2</name>
        <name evidence="7" type="ordered locus">Rmet_1239</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08125.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516012.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1239"/>
    <dbReference type="EnsemblBacteria" id="ABF08125">
        <property type="protein sequence ID" value="ABF08125"/>
        <property type="gene ID" value="Rmet_1239"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1239"/>
    <dbReference type="eggNOG" id="COG4974">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027562_23_3_4"/>
    <dbReference type="OMA" id="VAVHKLR"/>
    <dbReference type="OrthoDB" id="745068at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.443.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044068">
        <property type="entry name" value="CB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011010">
        <property type="entry name" value="DNA_brk_join_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013762">
        <property type="entry name" value="Integrase-like_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002104">
        <property type="entry name" value="Integrase_catalytic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010998">
        <property type="entry name" value="Integrase_recombinase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004107">
        <property type="entry name" value="Integrase_SAM-like_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02899">
        <property type="entry name" value="Phage_int_SAM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00589">
        <property type="entry name" value="Phage_integrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56349">
        <property type="entry name" value="SSF56349"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51900">
        <property type="entry name" value="CB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51898">
        <property type="entry name" value="TYR_RECOMBINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0229">DNA integration</keyword>
    <keyword evidence="3" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="2 4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Core-binding (CB)" evidence="6">
        <location>
            <begin position="122"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="domain" description="Tyr recombinase" evidence="5">
        <location>
            <begin position="229"/>
            <end position="412"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABF08125.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="419" mass="47621" checksum="2847031AA1C9F966" modified="2006-05-30" version="1">MLEHHFRSLKAIDRVRAQWLGPQIERYVQSLDELHTSNATVRQHIRALGHFNDFVIARGVTKLEALPDHVDAFVEYWHAGHAGWCKNNQDRAAVTAQSRVPVERMLCLALPDYTRPNNRPAMPFANTVPGFLPFLNDEKGLRQESQQRYLYTLRPFEAYLNRGGIALSGLEPAHISRFLEERAKTLHKAGMRDTAGALRIFLRYLHREGIVATDLVRSVPRGRIYRQASIPRAIGWQDVERLLTSIDRRSILGKRDYAILTLLASYGLRAREIAALCLDDFDWTHAQISIPMRKGGHSTRYPLSATVGDAVISYLQARRSDVPHRQVFLTARSPYIPMRHWTVSAMVGIRMRDIGIQVTRAGSHTLRHACVQHLVEADMPFKVIGDYVGHSHPSSTLVYGKVAVHKLRQIVLFQGEDVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="69" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LP82</accession>
    <name>Q1LP82_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_1158</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08044.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008650501.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1158"/>
    <dbReference type="EnsemblBacteria" id="ABF08044">
        <property type="protein sequence ID" value="ABF08044"/>
        <property type="gene ID" value="Rmet_1158"/>
    </dbReference>
    <dbReference type="GeneID" id="60822027"/>
    <dbReference type="KEGG" id="rme:Rmet_1158"/>
    <dbReference type="eggNOG" id="COG0251">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_100715_6_1_4"/>
    <dbReference type="OMA" id="WDEWVAQ"/>
    <dbReference type="OrthoDB" id="1633707at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd06150">
        <property type="entry name" value="YjgF_YER057c_UK114_like_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035959">
        <property type="entry name" value="RutC-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006175">
        <property type="entry name" value="YjgF/YER057c/UK114"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035709">
        <property type="entry name" value="YoaB-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47328">
        <property type="entry name" value="PTHR47328"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01042">
        <property type="entry name" value="Ribonuc_L-PSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55298">
        <property type="entry name" value="SSF55298"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF08044.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="120" mass="13250" checksum="0F9B677A23188A55" modified="2006-05-30" version="1">MSTNTLKRAHVGKRLSEYAVYNGVVYLAGQVPEVDPKADLRGQTREVLGHIDRLLAEAGSDKTRILMCQIFLTDITRIGEMNEVWDEWVAQGNTPPRATVQAALANPDYLIEVVVTAALN</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LP84</accession>
    <name>Q1LP84_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">D-beta-hydroxybutyrate dehydrogenase</fullName>
            <ecNumber evidence="2">1.1.1.30</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">bdhA</name>
        <name evidence="2" type="ordered locus">Rmet_1156</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.30" evidence="2"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08042.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515941.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1156"/>
    <dbReference type="EnsemblBacteria" id="ABF08042">
        <property type="protein sequence ID" value="ABF08042"/>
        <property type="gene ID" value="Rmet_1156"/>
    </dbReference>
    <dbReference type="GeneID" id="60822025"/>
    <dbReference type="KEGG" id="rme:Rmet_1156"/>
    <dbReference type="eggNOG" id="COG1028">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010194_1_0_4"/>
    <dbReference type="OMA" id="QHGKIDN"/>
    <dbReference type="OrthoDB" id="1094766at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003858">
        <property type="term" value="F:3-hydroxybutyrate dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011294">
        <property type="entry name" value="3-OHbutyrate_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01963">
        <property type="entry name" value="PHB_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00061">
        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU000363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF08042.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="258" mass="27100" checksum="568EA78B1266E386" modified="2006-05-30" version="1">MLKGKTALVTGSTSGIGLGIAQALAAQGANIIVNGFGDVDGAKAEIAKSGSGIQVGYHGADMSKAAEIEDMMRYAEKDFGGVDILVNNAGIQFVAKIEEFPVDRWDAIIAINLTSAFHTTRLALPSMKAKNWGRVINIASTHGLVASAEKSAYVAAKHGIVGFTKVTALETAQTGVTANAICPGWVLTPLVQKQVEARAQKEGIPVEQAKRELVIEKQPSGQFVTPEELGALAVFLSSEAARQVRGAIWNMDGGWVAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="72" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LPA1</accession>
    <name>Q1LPA1_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Putative MFS transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Rmet_1139</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08025.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1139"/>
    <dbReference type="EnsemblBacteria" id="ABF08025">
        <property type="protein sequence ID" value="ABF08025"/>
        <property type="gene ID" value="Rmet_1139"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1139"/>
    <dbReference type="eggNOG" id="COG2814">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013133_6_0_4"/>
    <dbReference type="OMA" id="HGPYYTF"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026032">
        <property type="entry name" value="HcaT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024989">
        <property type="entry name" value="MFS_assoc_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12832">
        <property type="entry name" value="MFS_1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004925">
        <property type="entry name" value="HcaT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="SSF103473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="20"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="51"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="75"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="114"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="139"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="183"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="216"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="242"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="268"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="309"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="337"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="domain" description="MFS_1_like" evidence="6">
        <location>
            <begin position="2"/>
            <end position="340"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF12832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABF08025.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="365" mass="39621" checksum="2E58652232890F69" modified="2006-05-30" version="1">MISPYVSLFFADRGFSPVQIGVLMACFQLSRIVGPYLWGWLSDVMHTRVKLLRLAAVTALLAFLAVPGIQSYGGMMAMMIGLNLITSAMSPLGDALTISTLRRHGAFDHRYGRVRMFGSIGFIAAVLTGGALFERFGMQAFPWLASTMLALFALVVFGMRDAADEGPRAAPPPALPLLRRPDVAWFFASAFLMMFAHAALYVFYSLYLEQLGYSKFAIGVMWTIGVVAEIVFFFYQGKLFGALALRTILAGTFVLAALRFGMTGYFAQYAWLIALVQILHAATFGAHHSASLKRLQRWFAGPLQGRGQALYTGISYGVGGTLGGLAMGWTWKTLAPMHTFGLAALAALIGAGCAVMSFRGEPERA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LPC1</accession>
    <name>Q1LPC1_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">FecR-like transmembrane sensor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_1119</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08005.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1119"/>
    <dbReference type="EnsemblBacteria" id="ABF08005">
        <property type="protein sequence ID" value="ABF08005"/>
        <property type="gene ID" value="Rmet_1119"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1119"/>
    <dbReference type="eggNOG" id="COG3712">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_050192_0_2_4"/>
    <dbReference type="OMA" id="WALCLGE"/>
    <dbReference type="OrthoDB" id="1381035at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006860">
        <property type="entry name" value="FecR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032623">
        <property type="entry name" value="FecR_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012373">
        <property type="entry name" value="Ferrdict_sens_TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30273">
        <property type="entry name" value="PTHR30273"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16220">
        <property type="entry name" value="DUF4880"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04773">
        <property type="entry name" value="FecR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF018266">
        <property type="entry name" value="FecR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <feature type="domain" description="DUF4880" evidence="2">
        <location>
            <begin position="20"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="domain" description="FecR" evidence="1">
        <location>
            <begin position="132"/>
            <end position="223"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF04773"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF16220"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF08005.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="357" mass="37793" checksum="8A449CBDEA6A3B4D" modified="2006-05-30" version="1">MQRSCAPVENPMSQSVDVREAAARWFSRNQAPALSPAEQQAFAAWLAASAEHRAEYAALERVWQAAAAIDPDRLRKLAVDESACPTPPLSPPISRRVSAGWRPALLGLCAALAIGIGWIGWQHDAAVATNAEYQTAPGERRTLTLDDGSRIELNTRSRLEIHYSAGTRRVTLREGEAMFEVSHDAARPFVVDAGMGTVTVTGTRFDVRRDTSGVDVSVESGSVDVRGSVRGSVRGSAKDSVKGNAGRTPDTRLSAGLGARIASDGTVTGAASIDLPGVLAWREGKLVFNDAPLSAVAAEVSRYRQQPVRVADAATGSLRVSSVFSADDTSGLLAALPQMLPVGVRNLPDGSTEIFSH</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="99" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LPD2</accession>
    <name>Q1LPD2_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Ferrichrome receptor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">fcuA</name>
        <name evidence="4" type="ordered locus">Rmet_1108</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TonB-dependent receptor family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07994.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1108"/>
    <dbReference type="EnsemblBacteria" id="ABF07994">
        <property type="protein sequence ID" value="ABF07994"/>
        <property type="gene ID" value="Rmet_1108"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1108"/>
    <dbReference type="eggNOG" id="COG4774">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008287_22_0_4"/>
    <dbReference type="OMA" id="DSYWASA"/>
    <dbReference type="OrthoDB" id="196825at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009279">
        <property type="term" value="C:cell outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015344">
        <property type="term" value="F:siderophore uptake transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038023">
        <property type="term" value="F:signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.130.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.170.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030149">
        <property type="entry name" value="FcuA/FatA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012910">
        <property type="entry name" value="Plug_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037066">
        <property type="entry name" value="Plug_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039423">
        <property type="entry name" value="TonB-dep_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036942">
        <property type="entry name" value="TonB_rcpt_b-brl_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010105">
        <property type="entry name" value="TonB_sidphr_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32552">
        <property type="entry name" value="PTHR32552"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32552:SF82">
        <property type="entry name" value="PTHR32552:SF82"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07715">
        <property type="entry name" value="Plug"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01783">
        <property type="entry name" value="TonB-siderophor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0675">Receptor</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Plug" evidence="3">
        <location>
            <begin position="120"/>
            <end position="212"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009810"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF07715"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF07994.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="761" mass="81652" checksum="B172DF78A6C2B5C3" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="67" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LPL7</accession>
    <name>Q1LPL7_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Site-specific recombinase Gcr</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_1023</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07909.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515818.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1023"/>
    <dbReference type="EnsemblBacteria" id="ABF07909">
        <property type="protein sequence ID" value="ABF07909"/>
        <property type="gene ID" value="Rmet_1023"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1023"/>
    <dbReference type="eggNOG" id="COG4389">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023672_0_0_4"/>
    <dbReference type="OMA" id="HYITRDR"/>
    <dbReference type="OrthoDB" id="161332at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011385">
        <property type="entry name" value="Site-sp_rcmbase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10136">
        <property type="entry name" value="SpecificRecomb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015380">
        <property type="entry name" value="Site-sp_rcmb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="373"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="406"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="516"/>
            <end position="535"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="574"/>
            <end position="594"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="606"/>
            <end position="625"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="631"/>
            <end position="657"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF07909.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="703" mass="77477" checksum="9F68D5D1C51EE484" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="70" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LPM3</accession>
    <name>Q1LPM3_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Intracellular poly(3-hydroxybutyrate) depolymerase</fullName>
            <ecNumber evidence="2">3.1.1.75</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">phaZ1</name>
        <name evidence="2" type="ordered locus">Rmet_1017</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EC" id="3.1.1.75" evidence="2"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07903.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008649308.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1017"/>
    <dbReference type="ESTHER" id="ralme-q1lpm3">
        <property type="family name" value="PHB_depolymerase_PhaZ"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF07903">
        <property type="protein sequence ID" value="ABF07903"/>
        <property type="gene ID" value="Rmet_1017"/>
    </dbReference>
    <dbReference type="GeneID" id="60821878"/>
    <dbReference type="KEGG" id="rme:Rmet_1017"/>
    <dbReference type="eggNOG" id="COG4553">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017495_0_0_4"/>
    <dbReference type="OMA" id="FYLQTVE"/>
    <dbReference type="OrthoDB" id="424537at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050526">
        <property type="term" value="F:poly(3-hydroxybutyrate) depolymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009656">
        <property type="entry name" value="PHB_depo_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010915">
        <property type="entry name" value="PHB_depoly_PhaZ"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06850">
        <property type="entry name" value="PHB_depo_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF020818">
        <property type="entry name" value="PHB_depoly_PhaZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="SSF53474"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01849">
        <property type="entry name" value="PHB_depoly_PhaZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PHB_depo_C" evidence="1">
        <location>
            <begin position="211"/>
            <end position="412"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF06850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF07903.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="414" mass="46747" checksum="20C31F63FCF82CCA" modified="2006-05-30" version="1">MLYQLHEFQRTMLNPLTAWAQATAKTFTNPLSPLSLVPGAPRLAAGYELLYRLGKAYEKPEFNIKSVRSNGRDIPIVEQTILDKPFCKLVRFKRYADDPDTIKLLKDEPVVLVCAPLSGHHSTLLRDTVRTLLQDHKVYVTDWVDARMVPVEHGEFHLSDYIRYIQEFIRHIGAENLNVISVCQPTVPVLAAISLMASAGEKTPRTMTMMGGPIDARKSATAVNSLATNKSYEWFENNVIYTVPANYPGHGRRVYPGFLQHAGFVAMNPDRHVSSHYDYYLSLVQGGSDDAEAHVKFYDEYNAVLDMAAEYYLETIRTVFQEFRLANGTWVVDGEPVRPQDIKSTALLTIEGELDDISGAGQTAAAHDLCTGIAKTRKQHLTAEKCGHYGIFSGRRWRDSVYPELRDFIRKYRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="68" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LQL1</accession>
    <name>Q1LQL1_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_0679</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07565.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515534.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0679"/>
    <dbReference type="EnsemblBacteria" id="ABF07565">
        <property type="protein sequence ID" value="ABF07565"/>
        <property type="gene ID" value="Rmet_0679"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0679"/>
    <dbReference type="eggNOG" id="COG3146">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036032_1_0_4"/>
    <dbReference type="OMA" id="HFEVCYY"/>
    <dbReference type="OrthoDB" id="891566at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007434">
        <property type="entry name" value="FemAB-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04339">
        <property type="entry name" value="FemAB_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="SSF55729"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF07565.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="400" mass="45732" checksum="95D5F04032FA16A0" modified="2006-05-30" version="1">MSESRLSPNYRTEIVQDLTNIDANDWDALLAAQAEPTPFLRHAFLQALHASGSGSATDDTGWSPRFLALWVPDGKEPGRDRLAAAMPLYAKSHSYGEYVFDWAWADAYRQHGIPYYPKWLAAIPFTPVRGARLLAEDELSRRVLLRFALALAQESELSSLHVLFPSDHEADLMDEAGMMMRHGVQFHWTNPGYENFDAFLATLSQKKRKNIRAERRRVTEAGITFRHLRGADIDDDAWKLFNRCYRQTYRDHHSTPYLNLDFFRRIGAAMPEHLLLVIAEREGRDIASSLLVYDADPAVSTLYGRYWGALEHHPCLHFETAYYQPLEFCIREGIGTFEGGAQGEHKMARGFLPVATRSAHWLAHPSFADAVERFLERERQGIDAYMDELNERNPFAGARE</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LR82</accession>
    <name>Q1LR82_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Universal stress protein, UspA family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">uspA1</name>
        <name evidence="3" type="ordered locus">Rmet_0458</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal stress protein A family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07344.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515332.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0458"/>
    <dbReference type="EnsemblBacteria" id="ABF07344">
        <property type="protein sequence ID" value="ABF07344"/>
        <property type="gene ID" value="Rmet_0458"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0458"/>
    <dbReference type="eggNOG" id="COG0589">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_049301_14_0_4"/>
    <dbReference type="OMA" id="WHREQND"/>
    <dbReference type="OrthoDB" id="2040681at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006015">
        <property type="entry name" value="Universal_stress_UspA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006016">
        <property type="entry name" value="UspA"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00582">
        <property type="entry name" value="Usp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01438">
        <property type="entry name" value="UNVRSLSTRESS"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Usp" evidence="2">
        <location>
            <begin position="3"/>
            <end position="140"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008791"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF07344.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="144" mass="15091" checksum="197428B2C6AAAD99" modified="2006-05-30" version="1">MPQIVLATDGSAFSDAAARFLADGKLLQTGFTVHVIHITPDVTGQVRAFVSKETIDAWHQEESGKAMASVCEILSAAKVPFEQHALHGFPPEKIVAYAKSVDAGAIVMGTHGRGSFLDAVIGSVAGRVLAHAGCPVIMVKPEKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="115" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LRW0</accession>
    <name>Q1LRW0_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Response regulator of C4-dicarboxylate transportsystem</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">dctD</name>
        <name evidence="9" type="ordered locus">Rmet_0230</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07116.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0230"/>
    <dbReference type="EnsemblBacteria" id="ABF07116">
        <property type="protein sequence ID" value="ABF07116"/>
        <property type="gene ID" value="Rmet_0230"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0230"/>
    <dbReference type="eggNOG" id="COG2204">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_0_6_4"/>
    <dbReference type="OMA" id="MPISMQV"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002197">
        <property type="entry name" value="HTH_Fis"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002078">
        <property type="entry name" value="Sigma_54_int"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025662">
        <property type="entry name" value="Sigma_54_int_dom_ATP-bd_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025943">
        <property type="entry name" value="Sigma_54_int_dom_ATP-bd_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025944">
        <property type="entry name" value="Sigma_54_int_dom_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02954">
        <property type="entry name" value="HTH_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00158">
        <property type="entry name" value="Sigma54_activat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01590">
        <property type="entry name" value="HTHFIS"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00675">
        <property type="entry name" value="SIGMA54_INTERACT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00676">
        <property type="entry name" value="SIGMA54_INTERACT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00688">
        <property type="entry name" value="SIGMA54_INTERACT_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50045">
        <property type="entry name" value="SIGMA54_INTERACT_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Response regulatory" evidence="8">
        <location>
            <begin position="9"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="domain" description="Sigma-54 factor interaction" evidence="7">
        <location>
            <begin position="148"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="6">
        <location>
            <position position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50045"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ABF07116.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="444" mass="48193" checksum="482DEC7F3742F7AE" modified="2006-05-30" version="1">MIAMQDGLRVLFVEDEPLVRQATAQSLELAGFSVLALPSAEAAMPHLGADFPGVLVTDVRLSGASGLDLLQHCRNAAPGVPVILVTGHGDITMAVQAMREGAYDFIEKPFGADRLTETVRRALERRALELENHALRRELAGPAAGTRIIGRSPAIAQVRDLIANVAATDVPVMINGETGTGKELVARSLHALSTRRDQPFIALNCGAVPESIFESEMFGHEAGAFTGAGKRRVGKLEHASGGTLFLDEIESMPLALQVKLLRVLQEGTLERLGSNTSIPIDVRIIAASKGDMEALVAQGTFRQDLLYRLNVVTIPLPPLRERREDIVPLFEHFMLVAAVRYQRPAPILSEMQRQQLMQRPWPGNVRELRNAADRLVLGVPEGGHAGGKADPVDESTPLRERMERYERAVIADTLARTGGAVSQAADLLQVGKATLYDKIKRYGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LRX6</accession>
    <name>Q1LRX6_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Long-chain-fatty-acid--CoA ligase (Long-chain acyl-CoA synthetase)</fullName>
            <ecNumber evidence="2">6.2.1.3</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_0214</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EC" id="6.2.1.3" evidence="2"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07100.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515119.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0214"/>
    <dbReference type="EnsemblBacteria" id="ABF07100">
        <property type="protein sequence ID" value="ABF07100"/>
        <property type="gene ID" value="Rmet_0214"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0214"/>
    <dbReference type="eggNOG" id="COG1022">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000022_45_5_4"/>
    <dbReference type="OMA" id="RGMRKYD"/>
    <dbReference type="OrthoDB" id="164380at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004467">
        <property type="term" value="F:long-chain fatty acid-CoA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020845">
        <property type="entry name" value="AMP-binding_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_Synth/Lig"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00455">
        <property type="entry name" value="AMP_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0436">Ligase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AMP-binding" evidence="1">
        <location>
            <begin position="15"/>
            <end position="477"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF07100.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="654" mass="72496" checksum="0D647F01698A7178" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="96" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LRZ5</accession>
    <name>Q1LRZ5_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Bacterioferritin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">bfr</name>
        <name evidence="7" type="ordered locus">Rmet_0195</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="2">Oligomer of 24 subunits, arranged as 12 dimers, that are packed together to form an approximately spherical molecule with a central cavity, in which large amounts of iron can be deposited.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the bacterioferritin family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07081.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515102.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0195"/>
    <dbReference type="EnsemblBacteria" id="ABF07081">
        <property type="protein sequence ID" value="ABF07081"/>
        <property type="gene ID" value="Rmet_0195"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0195"/>
    <dbReference type="eggNOG" id="COG2193">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_104506_2_0_4"/>
    <dbReference type="OMA" id="NEEEHFH"/>
    <dbReference type="OrthoDB" id="1890523at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008199">
        <property type="term" value="F:ferric iron binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006879">
        <property type="term" value="P:cellular iron ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006826">
        <property type="term" value="P:iron ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00907">
        <property type="entry name" value="Bacterioferritin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1260.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002024">
        <property type="entry name" value="Bacterioferritin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012347">
        <property type="entry name" value="Ferritin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009040">
        <property type="entry name" value="Ferritin-like_diiron"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009078">
        <property type="entry name" value="Ferritin-like_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008331">
        <property type="entry name" value="Ferritin_DPS_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00210">
        <property type="entry name" value="Ferritin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002560">
        <property type="entry name" value="Bacterioferritin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00601">
        <property type="entry name" value="BACFERRITIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47240">
        <property type="entry name" value="SSF47240"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00754">
        <property type="entry name" value="bfr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50905">
        <property type="entry name" value="FERRITIN_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0349">Heme</keyword>
    <keyword evidence="4 5" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0409">Iron storage</keyword>
    <keyword evidence="4 5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ferritin-like diiron" evidence="6">
        <location>
            <begin position="1"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="18"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="50"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>3</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="51"/>
        </location>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="5">
        <location>
            <position position="52"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="54"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="94"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="130"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008093"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011713"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRNR" id="PIRNR002560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR002560-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50905"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABF07081.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="158" mass="18173" checksum="47C37DF65B98FD0C" modified="2006-05-30" version="1">MKADKKVIQLLNGQLKHELTAINQYFLHARMYRHWGLKELGKHEYEESIEEMKHADKLIERILMLDGLPNLQDLDKLLLGENTEEMLGCDLKLELASQVTSKDAIKYCESVGDYVSREILSEILSDTEDHIDWLQTQLELIGKIGLQNYQQSAMGGVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LS02</accession>
    <name>Q1LS02_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Type II secretion system protein H</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">General secretion pathway protein H</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ordered locus">Rmet_0188</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell inner membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07074.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515094.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0188"/>
    <dbReference type="EnsemblBacteria" id="ABF07074">
        <property type="protein sequence ID" value="ABF07074"/>
        <property type="gene ID" value="Rmet_0188"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0188"/>
    <dbReference type="eggNOG" id="COG4970">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_084761_1_0_4"/>
    <dbReference type="OMA" id="MRHIAKG"/>
    <dbReference type="OrthoDB" id="1621805at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015627">
        <property type="term" value="C:type II protein secretion system complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015628">
        <property type="term" value="P:protein secretion by the type II secretion system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012902">
        <property type="entry name" value="N_methyl_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045584">
        <property type="entry name" value="Pilin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022346">
        <property type="entry name" value="T2SS_GspH"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12019">
        <property type="entry name" value="GspH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07963">
        <property type="entry name" value="N_methyl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54523">
        <property type="entry name" value="SSF54523"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02532">
        <property type="entry name" value="IV_pilin_GFxxxE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00409">
        <property type="entry name" value="PROKAR_NTER_METHYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0488">Methylation</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="43"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="domain" description="GspH" evidence="12">
        <location>
            <begin position="84"/>
            <end position="209"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004377"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00021549"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00030775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF12019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ABF07074.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="222" mass="23174" checksum="F2B1DE72D1918207" modified="2006-05-30" version="1">MARLPHARLTGRGGRALAVRIEDADVDGVRASRANRKPSLRTVSGSAGLTLIEMAVTVAIVAIVAAISTPSFLSFIQSNRVQSEVNSMVNSLQLARSEAIKRGQSVSICPSSNGSSCLGANTWNVGWLVFVDRNADGVVDTPANAADLLQYRAPWAGTDTFVATPTATFVTYNRDGFGVNLPGGAVTLAAQTRPVSAQATRCIALNMVGRQTVQQSGVGACN</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LS17</accession>
    <name>Q1LS17_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative regulator of competence-specific genes, TfoX-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_0173</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07059.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515082.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0173"/>
    <dbReference type="EnsemblBacteria" id="ABF07059">
        <property type="protein sequence ID" value="ABF07059"/>
        <property type="gene ID" value="Rmet_0173"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0173"/>
    <dbReference type="eggNOG" id="COG3070">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_125849_3_0_4"/>
    <dbReference type="OMA" id="DLMAKWV"/>
    <dbReference type="OrthoDB" id="2040341at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007076">
        <property type="entry name" value="TfoX_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04993">
        <property type="entry name" value="TfoX_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TfoX_N" evidence="1">
        <location>
            <begin position="23"/>
            <end position="113"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF04993"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF07059.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="120" mass="13213" checksum="6862E59A3262524D" modified="2006-05-30" version="1">MPAARRPDPFVEHLLELMTPLAANIGAIAAKRMFGGHGLFYDGLMFALISDGQCFLKADDVTLARFEAEGSEPFRYQSARREVTMRHYLSLPAACLESPAAMTPWARMAVEAALRLGNAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="72" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LS36</accession>
    <name>Q1LS36_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Lauroyl/myristoyl acyltransferase</fullName>
            <ecNumber evidence="6">2.3.1.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">lpxL</name>
        <name evidence="6" type="ordered locus">Rmet_0154</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.3.1.-" evidence="6"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07040.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515067.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0154"/>
    <dbReference type="EnsemblBacteria" id="ABF07040">
        <property type="protein sequence ID" value="ABF07040"/>
        <property type="gene ID" value="Rmet_0154"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0154"/>
    <dbReference type="eggNOG" id="COG1560">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_049421_1_0_4"/>
    <dbReference type="OMA" id="IEIMPPL"/>
    <dbReference type="OrthoDB" id="1106624at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016746">
        <property type="term" value="F:acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009247">
        <property type="term" value="P:glycolipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="CDD" id="cd07984">
        <property type="entry name" value="LPLAT_LABLAT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004960">
        <property type="entry name" value="LipA_acyltrans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30606">
        <property type="entry name" value="PTHR30606"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03279">
        <property type="entry name" value="Lip_A_acyltrans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF026649">
        <property type="entry name" value="MsbB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="2" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="1" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABF07040.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="297" mass="33844" checksum="4DFD54505AEE8746" modified="2006-05-30" version="1">MSRIFTWLGIGLLTVLGKLPYPFVARFGEALGSILFMIPNPRREVVRANLRLCFPDKTDAEIEAMSRETYRKVFRSFAEGGIFWTCNEKKMRQLVQINDQGNLPSLDGSPHILVTLHLSGLEAGAIRLTMHLRDTVGRSGASLYTVQKNALFDDFLKVARGRFGANMISRNDSVRNILRVLKKGEALQLISDMDFGERDSEFVPFFGVEALTLTSVSRLARLAGAKVVPIYTEQLPDYKGYVLHILPPWDDYPGESVTDDTRRMNAFFEDAIRPRVPDYYWVHKRFKHRPPGEPDIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-04-26" modified="2022-08-03" version="99" xmlns="http://uniprot.org/uniprot">
    <accession>Q58AL7</accession>
    <name>Q58AL7_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Outer membrane protein (Porin)OmpC family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">ompP</name>
        <name evidence="7" type="ordered locus">Rmet_5974</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="7 8">
        <name>pMOL30</name>
    </geneLocation>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="8">Plasmid pMOL30</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000354">
        <property type="protein sequence ID" value="ABF12833.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011229350.1">
        <property type="nucleotide sequence ID" value="NC_007971.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_145601.1">
        <property type="nucleotide sequence ID" value="NC_006466.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF12833">
        <property type="protein sequence ID" value="ABF12833"/>
        <property type="gene ID" value="Rmet_5974"/>
    </dbReference>
    <dbReference type="GeneID" id="60820438"/>
    <dbReference type="KEGG" id="rme:Rmet_5974"/>
    <dbReference type="HOGENOM" id="CLU_038238_2_0_4"/>
    <dbReference type="OMA" id="DNQFNGR"/>
    <dbReference type="OrthoDB" id="1064531at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid pMOL30"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046930">
        <property type="term" value="C:pore complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015288">
        <property type="term" value="F:porin activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006811">
        <property type="term" value="P:ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00342">
        <property type="entry name" value="gram_neg_porins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.160.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033900">
        <property type="entry name" value="Gram_neg_porin_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023614">
        <property type="entry name" value="Porin_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002299">
        <property type="entry name" value="Porin_Neis"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13609">
        <property type="entry name" value="Porin_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00184">
        <property type="entry name" value="NEISSPPORIN"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0406">Ion transport</keyword>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-0626">Porin</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1134">Transmembrane beta strand</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5013531920" evidence="5">
        <location>
            <begin position="21"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="domain" description="Porin_4" evidence="6">
        <location>
            <begin position="7"/>
            <end position="319"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF13609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABF12833.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="355" mass="37720" checksum="0FA74059FF2A8F96" modified="2005-04-26" version="1">MKISRIAVASLGLFATTAFAQSSVTLYGVADAGIEYLSNVPSASPGGSNQVRMTSGNMSTSRWGLRGVEDLGGGLKAIFELESGISFDTGAQNNSTRLFDRSAFVGLGSKYGQLTLGRQTTPMYDTTLQLDPMGFAPRYSLFKMDDVLAGRADNAIKYRGIFSGLTVTGLYSFSRTGGGEVPGNYKVDRNMGVSLMYETGALAVGAVYDEIQGSTVATADRKDRRALIGASYAFGPAKAFIGYRWYNGNVGALPTNGSNIYWAGLRYGLTPALTLTGAAYYTDSRNSGADPFLFVASADYAFSKRTDVYMNVGYALNRGNSQLGMNGYNSTTGSPTNVVPGKDQTGVVVGVRHKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-01-04" modified="2022-01-19" version="95" xmlns="http://uniprot.org/uniprot">
    <accession>Q5NUW3</accession>
    <name>Q5NUW3_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">UBA/THIF-type NAD/FAD binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">thiF</name>
        <name evidence="2" type="ordered locus">Rmet_6235</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2 3">
        <name>pMOL28</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid pMOL28</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000355">
        <property type="protein sequence ID" value="ABF13094.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011239947.1">
        <property type="nucleotide sequence ID" value="NC_007972.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_161686.1">
        <property type="nucleotide sequence ID" value="NC_006525.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF13094">
        <property type="protein sequence ID" value="ABF13094"/>
        <property type="gene ID" value="Rmet_6235"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6235"/>
    <dbReference type="HOGENOM" id="CLU_070016_0_0_4"/>
    <dbReference type="OMA" id="YYLDCGN"/>
    <dbReference type="OrthoDB" id="1414532at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid pMOL28"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008641">
        <property type="term" value="F:ubiquitin-like modifier activating enzyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022500">
        <property type="entry name" value="PRTRC_ThiF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045886">
        <property type="entry name" value="ThiF/MoeB/HesA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000594">
        <property type="entry name" value="ThiF_NAD_FAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035985">
        <property type="entry name" value="Ubiquitin-activating_enz"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10953">
        <property type="entry name" value="PTHR10953"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00899">
        <property type="entry name" value="ThiF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69572">
        <property type="entry name" value="SSF69572"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03736">
        <property type="entry name" value="PRTRC_ThiF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ThiF" evidence="1">
        <location>
            <begin position="13"/>
            <end position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF13094.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="267" mass="28965" checksum="F6BE7A3A2CF6176F" modified="2005-01-04" version="1">MTFVHRIPEDMLLRAWRVSVVGAGGTGSALLPNLARLHHAMLELGHPGGIDCTVYDDDTVSETNVGRQGFYPVDVGQYKATLIVNRLNNLMGTRWEAHTRRVGSGDRFVCDMVIGCVDSRAARKAIVGAMQRGAGGYYLDCGNNADDGQVILGQIGGKAKERLPHVGDLYPQLLDPKGDKADTAPSCSMAEALQKQSLVINMAIAVQAYNLLWTLFRTGTLQNCGAFVNLLTGRTSPLPVDPVVWERFGYKLGARKARAKRKPAQSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-01-04" modified="2022-08-03" version="98" xmlns="http://uniprot.org/uniprot">
    <accession>Q5NUZ1</accession>
    <name>Q5NUZ1_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Histone-like DNA-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">bph2</name>
        <name evidence="4" type="ordered locus">Rmet_6191</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4 5">
        <name>pMOL28</name>
    </geneLocation>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="5">Plasmid pMOL28</plasmid>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the bacterial histone-like protein family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000355">
        <property type="protein sequence ID" value="ABF13050.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011239982.1">
        <property type="nucleotide sequence ID" value="NC_007972.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_161721.1">
        <property type="nucleotide sequence ID" value="NC_006525.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF13050">
        <property type="protein sequence ID" value="ABF13050"/>
        <property type="gene ID" value="Rmet_6191"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6191"/>
    <dbReference type="HOGENOM" id="CLU_105066_0_0_4"/>
    <dbReference type="OMA" id="RCASEFR"/>
    <dbReference type="OrthoDB" id="1847472at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid pMOL28"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030527">
        <property type="term" value="F:structural constituent of chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.520.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000119">
        <property type="entry name" value="Hist_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010992">
        <property type="entry name" value="IHF-like_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33175">
        <property type="entry name" value="PTHR33175"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00216">
        <property type="entry name" value="Bac_DNA_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00411">
        <property type="entry name" value="BHL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47729">
        <property type="entry name" value="SSF47729"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0614">Plasmid</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU003939"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF13050.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="140" mass="14764" checksum="AE700BAD8876FD5B" modified="2005-01-04" version="1">MATKKTAAPKTAAKKPAAPAKKAAPAAKKAAAAPVAKPIKDTFNKSSLLAHLVTQTELDKKTVQTVLAHLENTIASAIHKKGAGEFTFPGLFKVSAIQVPATKKRFGRNPFTGADQWFAAKPASVKVKVRPLKKLKDAAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-03-23" modified="2020-10-07" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>D3DXV4</accession>
    <name>D3DXV4_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_6516</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ADC45124.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADC45124">
        <property type="protein sequence ID" value="ADC45124"/>
        <property type="gene ID" value="Rmet_6516"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6516"/>
    <dbReference type="HOGENOM" id="CLU_2919461_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="38"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADC45124.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="61" mass="6535" checksum="04216E6B994416B6" modified="2010-03-23" version="1">MTPCAASLSLGTVACLSYVVDSNFIQGVDCAAHDTMPRASRPVMARTSRDHARPAQIRPAY</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-03-23" modified="2020-10-07" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>D3DXW5</accession>
    <name>D3DXW5_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_6528</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ADC45135.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_6528"/>
    <dbReference type="EnsemblBacteria" id="ADC45135">
        <property type="protein sequence ID" value="ADC45135"/>
        <property type="gene ID" value="Rmet_6528"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6528"/>
    <dbReference type="HOGENOM" id="CLU_3102965_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADC45135.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="51" mass="5436" checksum="B58F31069FD26D88" modified="2010-03-23" version="1">MESCALALPVLSVPSCDAEVVTAAPLAVKTCGVTLVMDVMFWFPIGREPIR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1990-01-01" modified="2022-05-25" version="141" xmlns="http://uniprot.org/uniprot">
    <accession>P13511</accession>
    <accession>P94142</accession>
    <accession>Q58AM3</accession>
    <name>CZCA_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName>Cobalt-zinc-cadmium resistance protein CzcA</fullName>
        </recommendedName>
        <alternativeName>
            <fullName>Cation efflux system protein CzcA</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">czcA</name>
        <name type="ordered locus">Rmet_5980</name>
    </gene>
    <organism>
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid">
        <name>pMOL30</name>
    </geneLocation>
    <reference key="1">
        <citation type="journal article" date="1989" name="Proc. Natl. Acad. Sci. U.S.A." volume="86" first="7351" last="7355">
            <title>Expression and nucleotide sequence of a plasmid-determined divalent cation efflux system from Alcaligenes eutrophus.</title>
            <authorList>
                <person name="Nies D.H."/>
                <person name="Nies A."/>
                <person name="Chu L."/>
                <person name="Silver S."/>
            </authorList>
            <dbReference type="PubMed" id="2678100"/>
            <dbReference type="DOI" id="10.1073/pnas.86.19.7351"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC DNA]</scope>
    </reference>
    <reference key="2">
        <citation type="submission" date="1996-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="van der Lelie D."/>
                <person name="Schwuchow T."/>
                <person name="Wuertz S."/>
                <person name="Schwidetzky U."/>
                <person name="Baeyens W."/>
                <person name="Scheel P.O."/>
                <person name="Nies D.H."/>
            </authorList>
        </citation>
        <scope>SEQUENCE REVISION TO 918 AND 959-960</scope>
    </reference>
    <reference key="3">
        <citation type="submission" date="2004-11" db="EMBL/GenBank/DDBJ databases">
            <title>Sequence and features of the Ralstonia metallidurans CH34 heavy metals plasmids pMOL28 and pMOL30.</title>
            <authorList>
                <person name="Monchy S."/>
                <person name="van der Lelie D."/>
                <person name="Vallaeys T."/>
                <person name="Taghavi S."/>
                <person name="Benotmane M."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="Lapidus A."/>
                <person name="Mergeay M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference key="4">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <reference key="5">
        <citation type="journal article" date="1995" name="J. Ind. Microbiol." volume="14" first="142" last="153">
            <title>The czc operon of Alcaligenes eutrophus CH34: from resistance mechanism to the removal of heavy metals.</title>
            <authorList>
                <person name="Diels L."/>
                <person name="Dong Q."/>
                <person name="van der Lelie D."/>
                <person name="Baeyens W."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="7766206"/>
            <dbReference type="DOI" id="10.1007/BF01569896"/>
        </citation>
        <scope>CHARACTERIZATION</scope>
        <scope>INDUCTION</scope>
        <scope>POSSIBLE MODE OF ACTION</scope>
    </reference>
    <comment type="function">
        <text>Has a low cation transport activity for cobalt, it is essential for the expression of cobalt, zinc, and cadmium resistance. CzcA and CzcB together would act in zinc efflux nearly as effectively as the complete CZC efflux system (CzcABC).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cell inner membrane</location>
            <topology evidence="4">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="induction">
        <text evidence="3">By zinc, cadmium and cobalt (zinc being the best and cobalt being the worst inducer).</text>
    </comment>
    <comment type="biotechnology">
        <text evidence="3">In the presence of 2 mM Cd(2+) or 4-10 mM Zn(2+) up to 99% of the metal is removed from the culture supernatant and is sequestered via bioprecipitation. Additionally Cu(2+), Co(2+), Ni(2+), Pb(2+), Y(3+) and Ge(4+) can also be removed from culture supernatants, although it is not clear which efflux system is responsible for all these substrates (PubMed:7766206).</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the resistance-nodulation-cell division (RND) (TC 2.A.6) family.</text>
    </comment>
    <dbReference type="EMBL" id="X98451">
        <property type="protein sequence ID" value="CAA67084.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="X71400">
        <property type="protein sequence ID" value="CAI11244.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP000354">
        <property type="protein sequence ID" value="ABF12839.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A33830">
        <property type="entry name" value="A33830"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011229347.1">
        <property type="nucleotide sequence ID" value="NC_007971.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_145595.1">
        <property type="nucleotide sequence ID" value="NC_006466.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="P13511"/>
    <dbReference type="SMR" id="P13511"/>
    <dbReference type="TCDB" id="2.A.6.1.2">
        <property type="family name" value="the resistance-nodulation-cell division (rnd) superfamily"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF12839">
        <property type="protein sequence ID" value="ABF12839"/>
        <property type="gene ID" value="Rmet_5980"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5980"/>
    <dbReference type="HOGENOM" id="CLU_002755_1_2_4"/>
    <dbReference type="OMA" id="PMALTFI"/>
    <dbReference type="OrthoDB" id="91817at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid pMOL30"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008324">
        <property type="term" value="F:cation transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046686">
        <property type="term" value="P:response to cadmium ion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2090.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027463">
        <property type="entry name" value="AcrB_DN_DC_subdom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001036">
        <property type="entry name" value="Acrflvin-R"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004763">
        <property type="entry name" value="CzcA/CusA/SilA/NccA/HelA/CnrA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32063">
        <property type="entry name" value="PTHR32063"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00873">
        <property type="entry name" value="ACR_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00702">
        <property type="entry name" value="ACRIFLAVINRP"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82714">
        <property type="entry name" value="SSF82714"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00914">
        <property type="entry name" value="2A0601"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0105">Cadmium resistance</keyword>
    <keyword id="KW-0997">Cell inner membrane</keyword>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0170">Cobalt</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-0614">Plasmid</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <keyword id="KW-0813">Transport</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <feature type="chain" id="PRO_0000161818" description="Cobalt-zinc-cadmium resistance protein CzcA">
        <location>
            <begin position="1"/>
            <end position="1063"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="14"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="350"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="371"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="395"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="452"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="487"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="534"/>
            <end position="554"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="883"/>
            <end position="903"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="906"/>
            <end position="926"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="937"/>
            <end position="957"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="981"/>
            <end position="1001"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="1013"/>
            <end position="1033"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1040"/>
            <end position="1063"/>
        </location>
    </feature>
    <feature type="sequence conflict" description="In Ref. 1; CAA67084." evidence="4" ref="1">
        <original>T</original>
        <variation>I</variation>
        <location>
            <position position="918"/>
        </location>
    </feature>
    <feature type="sequence conflict" description="In Ref. 1; CAA67084." evidence="4" ref="1">
        <original>SL</original>
        <variation>LV</variation>
        <location>
            <begin position="959"/>
            <end position="960"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="3">
        <source>
            <dbReference type="PubMed" id="7766206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="4"/>
    <sequence length="1063" mass="115644" checksum="47AF0AA18E0D4D2E" modified="2006-07-11" version="3"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-10-07" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>Q1L9I9</accession>
    <name>Q1L9I9_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_6328</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="1 2">
        <name>pMOL28</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid pMOL28</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000355">
        <property type="protein sequence ID" value="ABF13187.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF13187">
        <property type="protein sequence ID" value="ABF13187"/>
        <property type="gene ID" value="Rmet_6328"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6328"/>
    <dbReference type="HOGENOM" id="CLU_2438618_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid pMOL28"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF13187.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="90" mass="10020" checksum="56750207A43287D8" modified="2006-05-30" version="1">MADEFPVGVLVLDDAAVYSLRIIDEGGGRYRGRYVWHKGTDLASVEALLCAKWLPEAEWHRTFGCFTSDDEVDCAVTIEIRNKLKNGATS</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-10-07" version="58" xmlns="http://uniprot.org/uniprot">
    <accession>Q1L9N8</accession>
    <name>Q1L9N8_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_6279</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2 3">
        <name>pMOL28</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid pMOL28</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000355">
        <property type="protein sequence ID" value="ABF13138.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF13138">
        <property type="protein sequence ID" value="ABF13138"/>
        <property type="gene ID" value="Rmet_6279"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6279"/>
    <dbReference type="HOGENOM" id="CLU_1056343_0_0_4"/>
    <dbReference type="OMA" id="WDKWLWS"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid pMOL28"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="127"/>
            <end position="155"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF13138.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="260" mass="28834" checksum="17409CF585A50D64" modified="2006-05-30" version="1">MSLFNLFKQAAISLLGHKNPTPPSAKVHVGTGADNEFQIHIGGVPHLFRDEGDRVVHFRAVFDRTIQHTAFGDKLLPEVMPRWSKVGVHDADGDTLQSVAERVVRDAKLSASVSRQEDSKAEVVRQHSTGLQKAGQLDDRAVHRPQTSASDASSSTYTVGTLLEWGEMEFPNRKPGGKRTYTSFAVKLDTGAGVKTLQGEGLKDVLADARCKVGERVGVKRLYKEKVPAFDQTTGQPLRDRTTGEQKLWDRWVWSINRIH</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-10-07" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>Q1L9S5</accession>
    <name>Q1L9S5_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_6242</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="1 2">
        <name>pMOL28</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid pMOL28</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000355">
        <property type="protein sequence ID" value="ABF13101.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF13101">
        <property type="protein sequence ID" value="ABF13101"/>
        <property type="gene ID" value="Rmet_6242"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6242"/>
    <dbReference type="HOGENOM" id="CLU_1843478_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid pMOL28"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF13101.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="139" mass="14932" checksum="73DCEC3C24EAB61E" modified="2006-05-30" version="1">MLLRDYKMTKPFTLFNAAALGKSQPIVDVGEPEPVVGIATDDYIIALANLTNIVVSLQRMKVGNNPWTAFTPMAPIGRTCSYGDPKKCADEGKYQKVTVIGCGGASFSIELRNHDGEGAVWDGLYADCQYGGGVLFLVP</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-10-07" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LA87</accession>
    <name>Q1LA87_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_6080</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="1 2">
        <name>pMOL30</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid pMOL30</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000354">
        <property type="protein sequence ID" value="ABF12939.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF12939">
        <property type="protein sequence ID" value="ABF12939"/>
        <property type="gene ID" value="Rmet_6080"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6080"/>
    <dbReference type="HOGENOM" id="CLU_515671_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid pMOL30"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF12939.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="528" mass="58758" checksum="464979660CBBC8A6" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="103" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LB47</accession>
    <name>Q1LB47_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 9">2-dehydropantoate 2-reductase</fullName>
            <ecNumber evidence="4 9">1.1.1.169</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8 9">Ketopantoate reductase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="primary">panE</name>
        <name evidence="12" type="ordered locus">Rmet_5770</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="13">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="13">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Catalyzes the NADPH-dependent reduction of ketopantoate into pantoic acid.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 9">
            <text>(R)-pantoate + NADP(+) = 2-dehydropantoate + H(+) + NADPH</text>
            <dbReference type="Rhea" id="RHEA:16233"/>
            <dbReference type="ChEBI" id="CHEBI:11561"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15980"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.1.1.169"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 9">Cofactor biosynthesis; (R)-pantothenate biosynthesis; (R)-pantoate from 3-methyl-2-oxobutanoate: step 2/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the ketopantoate reductase family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.169" evidence="4 9"/>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12629.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011520169.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5770"/>
    <dbReference type="EnsemblBacteria" id="ABF12629">
        <property type="protein sequence ID" value="ABF12629"/>
        <property type="gene ID" value="Rmet_5770"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5770"/>
    <dbReference type="eggNOG" id="COG1893">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031468_6_1_4"/>
    <dbReference type="OMA" id="WEKWVFL"/>
    <dbReference type="OrthoDB" id="427052at2"/>
    <dbReference type="UniPathway" id="UPA00028">
        <property type="reaction ID" value="UER00004"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008677">
        <property type="term" value="F:2-dehydropantoate 2-reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015940">
        <property type="term" value="P:pantothenate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1040.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008927">
        <property type="entry name" value="6-PGluconate_DH-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013328">
        <property type="entry name" value="6PGD_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003710">
        <property type="entry name" value="ApbA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013752">
        <property type="entry name" value="KPA_reductase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013332">
        <property type="entry name" value="KPR_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02558">
        <property type="entry name" value="ApbA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08546">
        <property type="entry name" value="ApbA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48179">
        <property type="entry name" value="SSF48179"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00745">
        <property type="entry name" value="apbA_panE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0520">NAD</keyword>
    <keyword evidence="9" id="KW-0521">NADP</keyword>
    <keyword evidence="9 12" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6 9" id="KW-0566">Pantothenate biosynthesis</keyword>
    <keyword evidence="12" id="KW-0614">Plasmid</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ApbA" evidence="10">
        <location>
            <begin position="3"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="domain" description="ApbA_C" evidence="11">
        <location>
            <begin position="179"/>
            <end position="299"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000784"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007870"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00019465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022655"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00032024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU362068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF02558"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF08546"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ABF12629.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="307" mass="32634" checksum="421F0B589D8B731F" modified="2006-05-30" version="1">MRILVVGVGATGGYFGARLLQAGRDVTFLVRPRRAAQLAEHGLRVKSPHGDLHFPNPPVVLSEQITQPYDVVLLSCKAYDLESAMDGFAPAMGPNTVIIPLLNGMQHLDRLDARFGAGRVMGGRCLISSTLDDQGTILHLSSFHVLSFGERPSGSSERALALAAAFSDAGFDVQRSDAMLQDMWEKWLFLATLASATCLMRATIGDILAAPRGRETIAALLGECAGVAAANGYPARGPVLEQAKTTLFAEGSPLTASMLRDLERGGAIESDQIVGDMLARRGQTAAPTLEMALTSLKAYEARRARGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LB60</accession>
    <name>Q1LB60_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Outer membrane protein (Porin)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Rmet_5757</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="7">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="7">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12616.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011520155.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5757"/>
    <dbReference type="EnsemblBacteria" id="ABF12616">
        <property type="protein sequence ID" value="ABF12616"/>
        <property type="gene ID" value="Rmet_5757"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5757"/>
    <dbReference type="eggNOG" id="COG3203">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038238_2_0_4"/>
    <dbReference type="OMA" id="IKAIFVL"/>
    <dbReference type="OrthoDB" id="1064531at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046930">
        <property type="term" value="C:pore complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015288">
        <property type="term" value="F:porin activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006811">
        <property type="term" value="P:ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00342">
        <property type="entry name" value="gram_neg_porins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.160.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033900">
        <property type="entry name" value="Gram_neg_porin_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023614">
        <property type="entry name" value="Porin_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13609">
        <property type="entry name" value="Porin_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0406">Ion transport</keyword>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-0626">Porin</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1134">Transmembrane beta strand</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193266" evidence="4">
        <location>
            <begin position="20"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="domain" description="Porin_4" evidence="5">
        <location>
            <begin position="7"/>
            <end position="351"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF13609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABF12616.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="387" mass="40902" checksum="DB21BC064D40DF76" modified="2006-05-30" version="1">MKKILFATAVLGAMGTAHAQSSVTLYGVADANIEYVSHLGSQPPTPANGFNAGQGGNTWRLTSGGLSSSRWGLRGVEDLGSGTKAVYVLESGFSIDNGVQSQGRLFGRQAYVGVDSRYGQFLFGRQYTSLFHIMANYSPTAYAVQYEPVVAFAGANLREDNTAKYIGTFGPVTAIAHWSFGTGVALPGTSQGAPSVGGNGEVPGQFRRDSAYGLGAEYAAGPFGVAVAYDQYNPSLSSAAGAFVGTGSFKKATIGASYTLGATAKIMGGYRWGQNKAQDGSTLILRDDFFWIGGNYQVTPALGLTLEYNYDNVKNLLGQTHLANPWQVSFIADYTLSKRTDLYLTTAFSKNAGLNFDTSAISFANGYFLASGQSTMLGVALGVRHKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LBE1</accession>
    <name>Q1LBE1_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transcriptional regulator, ArsR family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_5676</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12535.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011520081.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5676"/>
    <dbReference type="EnsemblBacteria" id="ABF12535">
        <property type="protein sequence ID" value="ABF12535"/>
        <property type="gene ID" value="Rmet_5676"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5676"/>
    <dbReference type="eggNOG" id="COG0640">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_097806_6_4_4"/>
    <dbReference type="OMA" id="CILANEE"/>
    <dbReference type="OrthoDB" id="1772343at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00090">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011991">
        <property type="entry name" value="ArsR-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001845">
        <property type="entry name" value="HTH_ArsR_DNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01022">
        <property type="entry name" value="HTH_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00778">
        <property type="entry name" value="HTHARSR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00418">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50987">
        <property type="entry name" value="HTH_ARSR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH arsR-type" evidence="1">
        <location>
            <begin position="13"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF12535.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="106" mass="11422" checksum="D88920C7EB777F5B" modified="2006-05-30" version="1">MNQTHSSIDLVSLQSAAADACALLKVLANPDRLLLMCQLSQGELSVGELEARLGIHQPTLSQQLAVLRENGLVVTRREGKNIIYAVASEQALAVMAVLYEQFCANS</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LBF2</accession>
    <name>Q1LBF2_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">NmrA-like protein/ Nucleoside-diphosphate-sugar epimerase (Exported)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_5665</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12524.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011520070.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5665"/>
    <dbReference type="EnsemblBacteria" id="ABF12524">
        <property type="protein sequence ID" value="ABF12524"/>
        <property type="gene ID" value="Rmet_5665"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5665"/>
    <dbReference type="eggNOG" id="COG0451">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_049717_0_0_4"/>
    <dbReference type="OMA" id="IGHQWAY"/>
    <dbReference type="OrthoDB" id="1928091at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001509">
        <property type="entry name" value="Epimerase_deHydtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01370">
        <property type="entry name" value="Epimerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Epimerase" evidence="1">
        <location>
            <begin position="6"/>
            <end position="216"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF12524.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="324" mass="35243" checksum="776F2A9618A8F78B" modified="2006-05-30" version="1">MSKVALFGAAGAIGRSIAAALRSRGEPYRVVGRHEASLRQAFGDDPLAEILTWNPDSPEAIQAAATGVETLIYLVGVNYWQFELHPALMRRTLDAAVAAGVKQIILIGTVYPYGRARTTPVSEDHPREPHTFKGRMRKSQEDLLMQAHADGRIRATVLRLPDFYGPGVENSLLHRAVLAAANGGTADLIGPIDTPHEFIFVPDVGPVVARLVDTPAAFGKIWHLAGAGVTTQRELVEEMERQTGRAMKLRVVGKTMLRLLGLFNPLMRELVEMHYLQTDPVIMDDSALRALIGPIRKTSYQDGIRQTLTAAMCANARLAPASAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LBM8</accession>
    <name>Q1LBM8_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Putative integral membrane protein putative MscS Mechanosensitive ion channel</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Rmet_5589</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="6">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="6">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12448.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519997.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5589"/>
    <dbReference type="EnsemblBacteria" id="ABF12448">
        <property type="protein sequence ID" value="ABF12448"/>
        <property type="gene ID" value="Rmet_5589"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5589"/>
    <dbReference type="eggNOG" id="COG0668">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_059701_1_0_4"/>
    <dbReference type="OrthoDB" id="1903165at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010920">
        <property type="entry name" value="LSM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006685">
        <property type="entry name" value="MscS_channel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023408">
        <property type="entry name" value="MscS_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00924">
        <property type="entry name" value="MS_channel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50182">
        <property type="entry name" value="SSF50182"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 4" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0614">Plasmid</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2 4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="51"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="76"/>
            <end position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABF12448.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="275" mass="30634" checksum="CCE86584DCAD6C42" modified="2006-05-30" version="1">MHFNANDSIVINVVTSLFVVLVGTMVVKLINRFFSARLQADNRGSYRAWTVASRNFVAAVTFLLLLGIWVSELKSVAISLAAFAAALLLVGKELVMCFLGAFMRMVTRPFQLGDLVEIGPFGGEVIDMDVMSTTLVEVAPARHYTGFTVQVPNSMLLTTAVRNHSQAGAYTLDTVRIPLEVGVDPEDVEARLIKVARQACEDFMEEAGRTLRRYGDMRFVDLSQFEPRVLFEPVDVDRFDAIVRFPAPVSARLPVAQQIVRGYYREPVRKNVAQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-10-07" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LBN6</accession>
    <name>Q1LBN6_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">GGDEF domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_5581</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12440.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5581"/>
    <dbReference type="EnsemblBacteria" id="ABF12440">
        <property type="protein sequence ID" value="ABF12440"/>
        <property type="gene ID" value="Rmet_5581"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5581"/>
    <dbReference type="eggNOG" id="COG3706">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025182_1_0_4"/>
    <dbReference type="OMA" id="RRYFQRE"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01949">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000160">
        <property type="entry name" value="GGDEF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031621">
        <property type="entry name" value="HisKA_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029787">
        <property type="entry name" value="Nucleotide_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035965">
        <property type="entry name" value="PAS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00990">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16927">
        <property type="entry name" value="HisKA_7TM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00267">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55073">
        <property type="entry name" value="SSF55073"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55785">
        <property type="entry name" value="SSF55785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00254">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50887">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="50"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="86"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="117"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="161"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="194"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="domain" description="GGDEF" evidence="2">
        <location>
            <begin position="405"/>
            <end position="540"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF12440.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="548" mass="59915" checksum="C5B87E0AEEAC1064" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="65" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LBP7</accession>
    <name>Q1LBP7_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_5570</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12429.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519979.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF12429">
        <property type="protein sequence ID" value="ABF12429"/>
        <property type="gene ID" value="Rmet_5570"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5570"/>
    <dbReference type="eggNOG" id="COG3831">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1467657_0_0_4"/>
    <dbReference type="OMA" id="WNWDTGG"/>
    <dbReference type="OrthoDB" id="1416846at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF12429.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="168" mass="18988" checksum="BE8672DC67F43C02" modified="2006-05-30" version="1">MAIELEDDAQDAVSRLFALINLGDTQQTTRQLTLLEEALEDAEEDDVDSEALLSQIKEIIDWESGFYVDWKDVESFIGCLNQLCERIDLEIDWGTDDTEDEDFLEGTSVPELMELAADQLRVAGYTLWNWDTGGDAYAGWITRSEDDDEMLEIADTLGFDARSGDQPF</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LBR6</accession>
    <name>Q1LBR6_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Extra-cytoplasmatic solute receptor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">bug</name>
        <name evidence="4" type="ordered locus">Rmet_5551</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="5">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="5">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Periplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the UPF0065 (bug) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12410.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5551"/>
    <dbReference type="EnsemblBacteria" id="ABF12410">
        <property type="protein sequence ID" value="ABF12410"/>
        <property type="gene ID" value="Rmet_5551"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5551"/>
    <dbReference type="eggNOG" id="COG3181">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045683_0_2_4"/>
    <dbReference type="OMA" id="QPAMSES"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042597">
        <property type="term" value="C:periplasmic space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005064">
        <property type="entry name" value="BUG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042100">
        <property type="entry name" value="Bug_dom1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42928">
        <property type="entry name" value="PTHR42928"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03401">
        <property type="entry name" value="TctC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017082">
        <property type="entry name" value="YflP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-0675">Receptor</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004192989" evidence="3">
        <location>
            <begin position="26"/>
            <end position="322"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF12410.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="322" mass="34529" checksum="85D96C8EA7E5E694" modified="2006-05-30" version="1">MRPRSLNLRAMLSLALFLASPFVFAKGYPDKPIRLIVPYAAGGSTDQLARIIQKPMSEYLGQPVVVENKPGAGGAIGTDVVAKSAPDGYTLVFGNTGPNAVIQLLRKVPYDTLRDLRPISTVAIAPMILAVPADSPAKSMKEFLAYARKQGTAMNIGSVGNGSLSHLTSEYFNEMAGLKMEHIPYTGGAPLMTAFLNGQVQAAFVTGLDGAVMVGSGKVRYLAVASPGRTEVVPGLPTMAEDVPGFKSQAWFGVLAPKGIPPEVEKKLRDAITKAVAQPEVRKIFIDRKVEPRSGSAEDLEKLIRDEIGQWNPVITKFNIRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LBT2</accession>
    <name>Q1LBT2_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Alpha-methylacyl-CoA racemase</fullName>
            <ecNumber evidence="2">5.1.99.4</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_5535</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EC" id="5.1.99.4" evidence="2"/>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12394.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519939.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5535"/>
    <dbReference type="EnsemblBacteria" id="ABF12394">
        <property type="protein sequence ID" value="ABF12394"/>
        <property type="gene ID" value="Rmet_5535"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5535"/>
    <dbReference type="eggNOG" id="COG1804">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033975_5_0_4"/>
    <dbReference type="OMA" id="HWCELLE"/>
    <dbReference type="OrthoDB" id="969868at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008111">
        <property type="term" value="F:alpha-methylacyl-CoA racemase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1540.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10540">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003673">
        <property type="entry name" value="CoA-Trfase_fam_III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044855">
        <property type="entry name" value="CoA-Trfase_III_dom3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023606">
        <property type="entry name" value="CoA-Trfase_III_dom_1_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02515">
        <property type="entry name" value="CoA_transf_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89796">
        <property type="entry name" value="SSF89796"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0413">Isomerase</keyword>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="335"/>
            <end position="373"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF12394.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="373" mass="40123" checksum="BF51C644FFC481C3" modified="2006-05-30" version="1">MARESDSPTLGPLAGLRVIEIAGIGPGPFACMLLSDLGAEVVRIDRSDAKLGDRFDIVGRGRKTVLLDLKKQTDVEKVLDLVSEADVLVEGFRPGVMERLGLGPADVEKRNPKLVYGRMTGWGQDGPLAQAAGHDINYIAISGALAGIGEAGRAPVPPLNLVGDYGGGSLYLVVGILAAVFEAQRSGKGQVVDAAICDGAVSLTSLFHTQLLRGHYVEKKASNTLDGGAPYYKSYETSDHQYISVGPIEPKFFALLCEKLGVPDDLQKSQNNRERWSELEKFFGRTFRTNTRAHWCELLEGTDACFAPVLTLSEAEMHPHLQYRKSFVSVGGVQQPAPAPRFSRTPGSVQGPAPQEAASLPDLLEAWRGGNRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="67" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LBX6</accession>
    <name>Q1LBX6_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Extra-cytoplasmic solute receptor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">bug</name>
        <name evidence="4" type="ordered locus">Rmet_5491</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="5">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="5">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Periplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the UPF0065 (bug) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12350.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519896.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5491"/>
    <dbReference type="EnsemblBacteria" id="ABF12350">
        <property type="protein sequence ID" value="ABF12350"/>
        <property type="gene ID" value="Rmet_5491"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5491"/>
    <dbReference type="eggNOG" id="COG3181">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045683_0_1_4"/>
    <dbReference type="OMA" id="GEANTTM"/>
    <dbReference type="OrthoDB" id="447734at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042597">
        <property type="term" value="C:periplasmic space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005064">
        <property type="entry name" value="BUG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042100">
        <property type="entry name" value="Bug_dom1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42928">
        <property type="entry name" value="PTHR42928"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03401">
        <property type="entry name" value="TctC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017082">
        <property type="entry name" value="YflP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-0675">Receptor</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193045" evidence="3">
        <location>
            <begin position="22"/>
            <end position="321"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF12350.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="321" mass="33759" checksum="9E6E7C3D50F194F4" modified="2006-05-30" version="1">MRYLKLPLMAACALVAGLAIAEEPYPSRPITIVVPYPAGGTSDVQVRKIQEPLSKLLGQPVLVDNKAGASGGIAAMAVARAKPDGYTFLYANNGLLIAPLLYKKSGYNPLKDFTPVGTVTSVPLVLVTNKAVPAKNLQEFLQYARKQPSGVRYASAGTVSYGHLATVRFGQLAGIKVDHIPYKGEAGTTFAAKAGEVEMLLTTPSPTMLAQVGPGGLTMLGVGTEKPSPVVPGVPTINQSVPGFTAEAWFGLLAPAGTPDAIVRKVNAAISTVMADKALQDWFIKTGAQAQPSTPDAFAAILKKETAQWQDIITKYKIEAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LC18</accession>
    <name>Q1LC18_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_5449</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12308.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519855.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5449"/>
    <dbReference type="EnsemblBacteria" id="ABF12308">
        <property type="protein sequence ID" value="ABF12308"/>
        <property type="gene ID" value="Rmet_5449"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5449"/>
    <dbReference type="eggNOG" id="COG5485">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_100997_5_0_4"/>
    <dbReference type="OMA" id="DYVVGQW"/>
    <dbReference type="OrthoDB" id="1907687at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030638">
        <property type="term" value="P:polyketide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009959">
        <property type="entry name" value="Cyclase_SnoaL-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032710">
        <property type="entry name" value="NTF2-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38436">
        <property type="entry name" value="PTHR38436"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07366">
        <property type="entry name" value="SnoaL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54427">
        <property type="entry name" value="SSF54427"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF12308.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="155" mass="16942" checksum="A8E1A451646B39BC" modified="2006-05-30" version="1">MSKEDDNKAVVGRWFTEFWGKNPNLAVIDEIAAPDMLLHYSLHEPRRGRADIRAFMTDFRKAFPDLNFWGTADLIAEGDYVIGQWEGGGTHTGPAHDDFLIGGLPAATGRKMHFTGTTVLKVIDGKIAEERGLDDGVTALTQLGLIKATTKNGDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LCC9</accession>
    <name>Q1LCC9_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative lipoprotein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_5338</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12197.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519740.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5338"/>
    <dbReference type="EnsemblBacteria" id="ABF12197">
        <property type="protein sequence ID" value="ABF12197"/>
        <property type="gene ID" value="Rmet_5338"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5338"/>
    <dbReference type="eggNOG" id="COG4259">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_139008_0_0_4"/>
    <dbReference type="OMA" id="YYWGDYQ"/>
    <dbReference type="OrthoDB" id="1969739at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014508">
        <property type="entry name" value="UCP020555_TPR-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16068">
        <property type="entry name" value="DUF4810"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF020555">
        <property type="entry name" value="UCP020555"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193303" evidence="2">
        <location>
            <begin position="22"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="120"/>
            <end position="145"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF12197.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="145" mass="15765" checksum="F8BD5CE107A94AC4" modified="2006-05-30" version="1">MMSMVTLRKAAFLSAAGLVLAGCASGPKPLYQWEGYQTQVYQYLKDGAKEEQVLALESGLDKMKAKGGSAPPGYHAQLGMLYLNLGKGDQMVKEFQTEKTLFPEATPYMDFLMRNVKTDKRPDTRAAAKADTTEAKSDATTGSAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="113" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LCF8</accession>
    <name>Q1LCF8_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Histidine kinase</fullName>
            <ecNumber evidence="2">2.7.13.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Rmet_5309</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="7">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="7">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.</text>
            <dbReference type="EC" id="2.7.13.3"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.7.13.3" evidence="2"/>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12168.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5309"/>
    <dbReference type="EnsemblBacteria" id="ABF12168">
        <property type="protein sequence ID" value="ABF12168"/>
        <property type="gene ID" value="Rmet_5309"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5309"/>
    <dbReference type="eggNOG" id="COG2203">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG3899">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG4191">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_34_2_4"/>
    <dbReference type="OMA" id="VPFEPFA"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00082">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041664">
        <property type="entry name" value="AAA_16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003018">
        <property type="entry name" value="GAF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029016">
        <property type="entry name" value="GAF-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003661">
        <property type="entry name" value="HisK_dim/P"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036097">
        <property type="entry name" value="HisK_dim/P_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004358">
        <property type="entry name" value="Sig_transdc_His_kin-like_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13191">
        <property type="entry name" value="AAA_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13185">
        <property type="entry name" value="GAF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00512">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00344">
        <property type="entry name" value="BCTRLSENSOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00065">
        <property type="entry name" value="GAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00388">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47384">
        <property type="entry name" value="SSF47384"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="SSF55874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="SSF56112"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="6" id="KW-0614">Plasmid</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="4">
        <location>
            <begin position="1"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="5">
        <location>
            <begin position="1524"/>
            <end position="1739"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABF12168.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="1744" mass="187170" checksum="F275945FB9D09F47" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="72" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LCH6</accession>
    <name>Q1LCH6_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_5291</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12150.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519695.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5291"/>
    <dbReference type="EnsemblBacteria" id="ABF12150">
        <property type="protein sequence ID" value="ABF12150"/>
        <property type="gene ID" value="Rmet_5291"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5291"/>
    <dbReference type="eggNOG" id="COG3246">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_065536_1_0_4"/>
    <dbReference type="OMA" id="YTAAHFI"/>
    <dbReference type="OrthoDB" id="1892901at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019475">
        <property type="term" value="P:L-lysine catabolic process to acetate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008567">
        <property type="entry name" value="KCE_family"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37418">
        <property type="entry name" value="PTHR37418"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05853">
        <property type="entry name" value="BKACE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF12150.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="311" mass="34221" checksum="9B8D548E7FBC39AD" modified="2006-05-30" version="1">MAAPQSKVIVTCAVTGSIHTPTMSPYLPITAREIEDEAVAAAEAGAAIVHLHARNPENGQPSQDPALFRAFLPRIAARSDVVINLTTGGAPNMSVEERLQPALQLKPEVASLNMGSMNFGLYPMLARHATFKHDWERPYLEGSEDRVFRNTFKEIRYILESCADNGTRFEIECYDTSHLYTAAHFIDRGILKPPFFIQSVFGLLGGIGTHADDVMHMRRTAERLFGKDYYWSVLGAGRSQMPIASMAAAMGGNVRVGLEDSLWEDRGKLSTSNADQVKRVVGILRSLNLDVASPDEARDMLQLKGRDAVAF</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LCN5</accession>
    <name>Q1LCN5_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Cytochrome bd ubiquinol oxidase, subunit I</fullName>
            <ecNumber evidence="13">1.10.3.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">cydA</name>
        <name evidence="13" type="ordered locus">Rmet_5232</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="14">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="14">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="12">Cell inner membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the cytochrome ubiquinol oxidase subunit 1 family.</text>
    </comment>
    <dbReference type="EC" id="1.10.3.-" evidence="13"/>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12091.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519637.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5232"/>
    <dbReference type="EnsemblBacteria" id="ABF12091">
        <property type="protein sequence ID" value="ABF12091"/>
        <property type="gene ID" value="Rmet_5232"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5232"/>
    <dbReference type="eggNOG" id="COG1271">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030555_3_1_4"/>
    <dbReference type="OMA" id="WAIIFNP"/>
    <dbReference type="OrthoDB" id="271551at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070069">
        <property type="term" value="C:cytochrome complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019646">
        <property type="term" value="P:aerobic electron transport chain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002585">
        <property type="entry name" value="Cyt-d_ubiquinol_oxidase_su_1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30365">
        <property type="entry name" value="PTHR30365"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01654">
        <property type="entry name" value="Cyt_bd_oxida_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006446">
        <property type="entry name" value="Cyt_quinol_oxidase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 12" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8 12" id="KW-0249">Electron transport</keyword>
    <keyword evidence="5 12" id="KW-0349">Heme</keyword>
    <keyword evidence="10 12" id="KW-0408">Iron</keyword>
    <keyword evidence="11 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="13" id="KW-0614">Plasmid</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 12" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="15"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="57"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="98"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="131"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="185"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="221"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="320"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="354"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="408"/>
            <end position="430"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRNR" id="PIRNR006446"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ABF12091.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="453" mass="49961" checksum="3E60FB2BB4D0FF88" modified="2006-05-30" version="1">MFASVDPVILARIQFAANITFHILFPTISISLAWVLLFFKLRYNKTGDENWMDAYRVWVKIFALTFALGVVSGITMSFQFGTNWPGYMETVGNIAGPLLAYEVLTAFFLEATFLGIMLFGMRRVSNRMHTIATLLVAGGTTLSAFWILALNSWMQTPAGFEMIDGRAHVTSWIEVIFNPSFPYRLVHMLLASGLTVAFLVAGISAYRWLRNDRSREVLHSMHTGVVLAAILIPLQIVAGDLHGLNTLHHQPAKIAAMEGIWKTEKGAPAVLFGLPNATTQSNDFEIAIPKLASIYLTHKVDGEIKGIDAFADRHPPVAPLFFAFRIMVGVGLLMLAASWVAAWQFRKRRAPAPWMARLLVAMTFSGWVALVAGWYVTEIGRQPYLVYGVLTTAQAASKVPATMIGSTLAMYLTLYAVLIVAYISVVFHLARKAGKSKDDSSQSVIPGQLRSAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-10-07" version="62" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LCU9</accession>
    <name>Q1LCU9_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Autotransporter domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_5168</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12027.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5168"/>
    <dbReference type="EnsemblBacteria" id="ABF12027">
        <property type="protein sequence ID" value="ABF12027"/>
        <property type="gene ID" value="Rmet_5168"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5168"/>
    <dbReference type="eggNOG" id="COG3468">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2603483_0_0_4"/>
    <dbReference type="OrthoDB" id="1713183at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019867">
        <property type="term" value="C:outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.128.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005546">
        <property type="entry name" value="Autotransporte_beta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036709">
        <property type="entry name" value="Autotransporte_beta_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006315">
        <property type="entry name" value="OM_autotransptr_brl"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03797">
        <property type="entry name" value="Autotransporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103515">
        <property type="entry name" value="SSF103515"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01414">
        <property type="entry name" value="autotrans_barl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Autotransporter" evidence="1">
        <location>
            <begin position="5"/>
            <end position="62"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03797"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF12027.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="79" mass="9112" checksum="F9F2A7C29072A873" modified="2006-05-30" version="1">MREEGKTKWQPYLTLNWWHTGVSSNISFNELPLGSMYPSNRYEVKIGANAQFSKRWTGWTNVSGRGARRASTTTRCVWA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LD32</accession>
    <name>Q1LD32_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transcriptional regulator, XRE family with cupin sensor domain</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_5085</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11944.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5085"/>
    <dbReference type="EnsemblBacteria" id="ABF11944">
        <property type="protein sequence ID" value="ABF11944"/>
        <property type="gene ID" value="Rmet_5085"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5085"/>
    <dbReference type="eggNOG" id="COG1396">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG3837">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_085376_3_0_4"/>
    <dbReference type="OMA" id="GQMSPTY"/>
    <dbReference type="OrthoDB" id="1292067at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00093">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001387">
        <property type="entry name" value="Cro/C1-type_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013096">
        <property type="entry name" value="Cupin_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010982">
        <property type="entry name" value="Lambda_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011051">
        <property type="entry name" value="RmlC_Cupin_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07883">
        <property type="entry name" value="Cupin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00530">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47413">
        <property type="entry name" value="SSF47413"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51182">
        <property type="entry name" value="SSF51182"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50943">
        <property type="entry name" value="HTH_CROC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH cro/C1-type" evidence="1">
        <location>
            <begin position="25"/>
            <end position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF11944.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="251" mass="26791" checksum="E98C45C725C76A01" modified="2006-05-30" version="1">MKETNASAHVDPSRTLDRETFGKRLRGARKALGWTLAHLAELSGVSITTISRAERGQLALGYDNFAAIGKALQMDMGEMFAGAGETAAPFQGPIVTRANQGVVYTGQSIAYEFLCTEAAGKQMSPTVATVHARHITGPEVFASHPGEEFIYVLSGEVEVHFANGEFVRLARGDSLYFDARLGHAYISVSRQLARTIGVTTSESQQMRSAQALPAAVSSKVKERRAVAAPGAKAVSGKRAGTVAKRAVKIKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LD54</accession>
    <name>Q1LD54_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Acetyl-CoA:acetoacetyl-CoA transferase, beta subunit</fullName>
            <ecNumber evidence="3">2.8.3.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">atoA1</name>
        <name evidence="3" type="ordered locus">Rmet_5060</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the 3-oxoacid CoA-transferase subunit B family.</text>
    </comment>
    <dbReference type="EC" id="2.8.3.-" evidence="3"/>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11922.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519472.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5060"/>
    <dbReference type="EnsemblBacteria" id="ABF11922">
        <property type="protein sequence ID" value="ABF11922"/>
        <property type="gene ID" value="Rmet_5060"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5060"/>
    <dbReference type="eggNOG" id="COG2057">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019942_4_1_4"/>
    <dbReference type="OMA" id="HADAFGM"/>
    <dbReference type="OrthoDB" id="1380130at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008410">
        <property type="term" value="F:CoA-transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012791">
        <property type="entry name" value="3-oxoacid_CoA-transf_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004165">
        <property type="entry name" value="CoA_trans_fam_I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004164">
        <property type="entry name" value="CoA_transf_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037171">
        <property type="entry name" value="NagB/RpiA_transferase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13707">
        <property type="entry name" value="PTHR13707"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01144">
        <property type="entry name" value="CoA_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00882">
        <property type="entry name" value="CoA_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100950">
        <property type="entry name" value="SSF100950"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02428">
        <property type="entry name" value="pcaJ_scoB_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01274">
        <property type="entry name" value="COA_TRANSF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF11922.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="233" mass="24542" checksum="B0A199F8DFF66B04" modified="2006-05-30" version="1">MHDTLPRLGTLPRLSRAQMARIVAHDIPDGSVVNLGIGMPTLVSDQLPDDREIVLHSENGILGMGPVIREGETDPDLINASKEPISLIAGASISDHAVSFTMMRGGHIDITVLGGFQVAANGDLANWSTGEPDAIPAVGGAMDLVVGAARLFVMMEHVTRDGQPKIVERCSYPLTGIGVVDRIYTDLAVIDVRPGKPLLVTGLVEGVSFETVQALSAAPLTLAADCRVLRAGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LD97</accession>
    <name>Q1LD97_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">NAD+-dependent betaine aldehyde dehydrogenase</fullName>
            <ecNumber evidence="3">1.2.1.8</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">betB</name>
        <name evidence="3" type="ordered locus">Rmet_5017</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EC" id="1.2.1.8" evidence="3"/>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11879.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519428.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5017"/>
    <dbReference type="EnsemblBacteria" id="ABF11879">
        <property type="protein sequence ID" value="ABF11879"/>
        <property type="gene ID" value="Rmet_5017"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5017"/>
    <dbReference type="eggNOG" id="COG1012">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005391_0_0_4"/>
    <dbReference type="OMA" id="AKTICIN"/>
    <dbReference type="OrthoDB" id="618655at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008802">
        <property type="term" value="F:betaine-aldehyde dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.309.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.605.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016161">
        <property type="entry name" value="Ald_DH/histidinol_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016163">
        <property type="entry name" value="Ald_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016162">
        <property type="entry name" value="Ald_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015590">
        <property type="entry name" value="Aldehyde_DH_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00171">
        <property type="entry name" value="Aldedh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53720">
        <property type="entry name" value="SSF53720"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aldedh" evidence="2">
        <location>
            <begin position="13"/>
            <end position="471"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF11879.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="473" mass="49570" checksum="55A6F6AB2119135B" modified="2006-05-30" version="1">MKTLDRFYIDGQWVTPAAAARSMPIVNPATEEVAGHVTLGSAEDVNRAVAAARAAFPAWSETSREERLALLERITALYKARLGDFAEAVRTEIGAPVSLCNTLQAPIGLAHLGAATETLRAFQFETAHGNNYVRHEPIGVAALITAWNWPLSLICAKVAAALAAGCTIVLKPSEIAPLDAALFAEVMHEAGTPAGVFNMVFGDGQEVGARLAAHPDVDVISITGSTRAGIAVAQAAAATVKRVHQELGGKSPLLILDDADLQAAVAQGVGHVMLNSGQTCIAPTRMLVPRDRYDEAVRVAAATANALQVGDPAEPGTRIGPISNKAQYEKVQRMIGTGIEEGARVVAGGPGRPDGLTRGFYARPTIFADVDNSMTIAREEIFGPVLVMIPYDDEAQGVDIANDTPYGLAAYVASGDPARARRVAARLRAGSVRINGGQVDLSMPFGGYRASGNGRENGAHGLAEYLEVKAVTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-10-07" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LDD0</accession>
    <name>Q1LDD0_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_4984</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11846.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4984"/>
    <dbReference type="EnsemblBacteria" id="ABF11846">
        <property type="protein sequence ID" value="ABF11846"/>
        <property type="gene ID" value="Rmet_4984"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4984"/>
    <dbReference type="eggNOG" id="COG0697">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033863_15_1_4"/>
    <dbReference type="OMA" id="IGWLVMS"/>
    <dbReference type="OrthoDB" id="1515911at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000620">
        <property type="entry name" value="EamA_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00892">
        <property type="entry name" value="EamA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="21"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="47"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="75"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="105"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="136"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="162"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="190"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="221"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="domain" description="EamA" evidence="2">
        <location>
            <begin position="18"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="domain" description="EamA" evidence="2">
        <location>
            <begin position="107"/>
            <end position="237"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF11846.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="255" mass="27012" checksum="2263393ED19A8037" modified="2006-05-30" version="1">MQSSSENDSYLRPGRISRRQLALAVAGGLAIVANWLLLFSAYSGASISIATAVYNTQPFMLVGLGALFLGERLTLARFGWLVLAFGGMLLIVRAGPDAGAMDGRYLVGILLALGAAFCYALAALIAKKLKDVPPHLIALIHVIVGTVTLLPMSLQADWPTGAATWTTLVTLGFVHTGVMYILLYGAIQKLSTSLTGALSFIYPIVAILVDRLAFGHHLHPSQIAGATLILLAAAGMTMGWSWRKRLPSAARKPSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LDP6</accession>
    <name>Q1LDP6_CUPMC</name>
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        <submittedName>
            <fullName evidence="2">Esterase/lipase/thioesterase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">llpE</name>
        <name evidence="2" type="ordered locus">Rmet_4868</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11730.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4868"/>
    <dbReference type="ESTHER" id="ralme-q1ldp6">
        <property type="family name" value="Hormone-sensitive_lipase_like"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF11730">
        <property type="protein sequence ID" value="ABF11730"/>
        <property type="gene ID" value="Rmet_4868"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4868"/>
    <dbReference type="eggNOG" id="COG0657">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012494_6_0_4"/>
    <dbReference type="OMA" id="TEFHVYP"/>
    <dbReference type="OrthoDB" id="1361074at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013094">
        <property type="entry name" value="AB_hydrolase_3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07859">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="SSF53474"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Abhydrolase_3" evidence="1">
        <location>
            <begin position="58"/>
            <end position="256"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF07859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF11730.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="291" mass="30991" checksum="14BE1C8866DB2C14" modified="2006-05-30" version="1">MTKTTTARSSAVQPAAEQGEPYVVQHMVVSGDREIKVCVCYPAGYWPLSAGSATLPWLVYLHAGGFVEGDVEAAKELARDLAESVPAVVVTPGYSLAPENPFPAAPEDAVATVTWVLKQAKKLKVDKLRFALVGEEAGGNLAIATAQMLRDRGLPQPAGQWLIRPVTDPCLSAMSSTRQVPMEMLRRLACNYREYLPTPADSVHPYAAPALASRMAGLPATLVQVAEQDALRAEGEAFAAKLAKAGISARALIMPGACGDGVEQSHEKCQAWVEEGARFLRDCFAKADDAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LDV5</accession>
    <name>Q1LDV5_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative acid phosphatase protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">acpA</name>
        <name evidence="2" type="ordered locus">Rmet_4809</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11671.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4809"/>
    <dbReference type="EnsemblBacteria" id="ABF11671">
        <property type="protein sequence ID" value="ABF11671"/>
        <property type="gene ID" value="Rmet_4809"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4809"/>
    <dbReference type="eggNOG" id="COG3511">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023677_0_0_4"/>
    <dbReference type="OMA" id="DLYHRFY"/>
    <dbReference type="OrthoDB" id="250274at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003993">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="2"/>
    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="acid_phos_Burk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193397" evidence="1">
        <location>
            <begin position="23"/>
            <end position="709"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF11671.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="709" mass="74832" checksum="98B722DD493C65ED" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LDW8</accession>
    <name>Q1LDW8_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Rhs element Vgr protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Rmet_4796</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="6">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="6">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11658.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4796"/>
    <dbReference type="EnsemblBacteria" id="ABF11658">
        <property type="protein sequence ID" value="ABF11658"/>
        <property type="gene ID" value="Rmet_4796"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4796"/>
    <dbReference type="eggNOG" id="COG3501">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG4253">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004121_4_0_4"/>
    <dbReference type="OMA" id="FAGWISE"/>
    <dbReference type="OrthoDB" id="1880900at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.230">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028244">
        <property type="entry name" value="T6SS_Rhs_Vgr_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017847">
        <property type="entry name" value="T6SS_RhsGE_Vgr_subset"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006533">
        <property type="entry name" value="T6SS_Vgr_RhsGE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037026">
        <property type="entry name" value="Vgr_OB-fold_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018769">
        <property type="entry name" value="VgrG2_C_DUF2345"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10106">
        <property type="entry name" value="DUF2345"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13296">
        <property type="entry name" value="T6SS_Vgr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01646">
        <property type="entry name" value="vgr_GE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03361">
        <property type="entry name" value="VI_Rhs_Vgr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0614">Plasmid</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="T6SS_Vgr" evidence="4">
        <location>
            <begin position="476"/>
            <end position="576"/>
        </location>
    </feature>
    <feature type="domain" description="DUF2345" evidence="3">
        <location>
            <begin position="596"/>
            <end position="734"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="892"/>
            <end position="911"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="556"/>
            <end position="604"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF10106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF13296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABF11658.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="911" mass="99347" checksum="AB7B3A7BE964D1C8" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LE19</accession>
    <name>Q1LE19_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Short-chain dehydrogenase/reductase SDR</fullName>
            <ecNumber evidence="1">1.1.1.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_4745</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EC" id="1.1.1.-" evidence="1"/>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11607.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008651283.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4745"/>
    <dbReference type="EnsemblBacteria" id="ABF11607">
        <property type="protein sequence ID" value="ABF11607"/>
        <property type="gene ID" value="Rmet_4745"/>
    </dbReference>
    <dbReference type="GeneID" id="60821665"/>
    <dbReference type="KEGG" id="rme:Rmet_4745"/>
    <dbReference type="eggNOG" id="COG1028">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010194_1_1_4"/>
    <dbReference type="OMA" id="ITYNSRA"/>
    <dbReference type="OrthoDB" id="1294334at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00061">
        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF11607.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="254" mass="26950" checksum="95441645739D1704" modified="2006-05-30" version="1">MSLFDLSGKVAIITGSSRGIGRAIAEQLAIQGAKVVVSSRKIEACQEVVDAINAKHGAGTALAVAANISSKDALQHLVDETNRVYGKVDVLVCNAASNPYYGPMSGVSDEQFRKVLDNNVISNHWLIQMVAPQMIERREGSIIIVSSIGGLRGSPTIGVYNVSKAADFQLARNLAVEFGPHNVRVNCIAPGLIKTDFARALWEDPVRYKQSTENAPLRRIGEPVEIAGAACYLASAASTFMTGQALVVDGGVTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="66" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LE25</accession>
    <name>Q1LE25_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative permeases of the major facilitator superfamily (NnrU)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">nnrU</name>
        <name evidence="3" type="ordered locus">Rmet_4739</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11601.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519173.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF11601">
        <property type="protein sequence ID" value="ABF11601"/>
        <property type="gene ID" value="Rmet_4739"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4739"/>
    <dbReference type="eggNOG" id="COG4094">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_104582_0_0_4"/>
    <dbReference type="OMA" id="APFVELW"/>
    <dbReference type="OrthoDB" id="1734625at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009915">
        <property type="entry name" value="NnrU_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07298">
        <property type="entry name" value="NnrU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="37"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="72"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="114"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="142"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="201"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="domain" description="NnrU" evidence="2">
        <location>
            <begin position="8"/>
            <end position="221"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07298"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF11601.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="230" mass="24665" checksum="90658B336DCD0052" modified="2006-05-30" version="1">MGSIGAVVVASVAFVGSHFLLSHPLRGALVRAVGEKGFLGIYSLVAFLTLGWMVMTYKRAPVSSPLWVPGDGIWAVVTVVMLIASILLMGSLIRNPALPTVGRPGAFPEAARGVFAVTRHPMMWAFALWGVCHIAVYPVTRNIVVALAMIVLALVGAALQDQKKERLLPDRWPDWEAKTSYLPFAAIVTGRARLAGFGSHALLGGLIVWLAATWAHLPLAAIAAGIWRWL</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="54" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LEF6</accession>
    <name>Q1LEF6_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_4608</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11470.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519045.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF11470">
        <property type="protein sequence ID" value="ABF11470"/>
        <property type="gene ID" value="Rmet_4608"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4608"/>
    <dbReference type="HOGENOM" id="CLU_123889_0_0_4"/>
    <dbReference type="OMA" id="DKHFDRC"/>
    <dbReference type="OrthoDB" id="1816600at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021333">
        <property type="entry name" value="DUF2946"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11162">
        <property type="entry name" value="DUF2946"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF11470.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="122" mass="12775" checksum="4EA7AE657ADCC654" modified="2006-05-30" version="1">MRRQSSLRTAWLGILAILLATLMPLASTLVSGAKLPVDTVCSAAGHQSGGQNEASLEDSFAAHFKHCGYCGLLAQLPALAATSIDTASAIVVTAAAVLPRLPAGVRHQRYLRRPSRAPPFSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="58" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LEH8</accession>
    <name>Q1LEH8_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_4586</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11448.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008648539.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4586"/>
    <dbReference type="EnsemblBacteria" id="ABF11448">
        <property type="protein sequence ID" value="ABF11448"/>
        <property type="gene ID" value="Rmet_4586"/>
    </dbReference>
    <dbReference type="GeneID" id="60825944"/>
    <dbReference type="KEGG" id="rme:Rmet_4586"/>
    <dbReference type="eggNOG" id="COG1062">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_073281_0_0_4"/>
    <dbReference type="OMA" id="MYGEAAF"/>
    <dbReference type="OrthoDB" id="913342at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193077" evidence="1">
        <location>
            <begin position="20"/>
            <end position="286"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF11448.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="286" mass="30978" checksum="3CB368AF075C5357" modified="2006-05-30" version="1">MKSALLSILLLGVLGNALADSAEPMAAPVTDGASAAEAAPVGPQLCDAPWFRSGARVQMEGDGTMPMSITMTLRDVMRTARGCSAQLEVRSKSALSALMGPPVVMDQVHEINIDRNSTRTRIDSRNATINAQARYARMFGGASFTGSGVFNYAGMTIRENTTLEGETFQSAVDLKIYPLGSDDMVGTMQAQHASIIVGSRHVGRKQMIDTVLGRKECMPITYEKRTSLGPLMIGDELLQLEPSVLHVTDWYCPTEAFVLRTEIRQNNKVQKVNVTALELTGDEDSH</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="97" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LEK5</accession>
    <name>Q1LEK5_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Transcriptional regulator, AsnC family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Rmet_4559</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="6">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="6">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11421.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008649536.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4559"/>
    <dbReference type="EnsemblBacteria" id="ABF11421">
        <property type="protein sequence ID" value="ABF11421"/>
        <property type="gene ID" value="Rmet_4559"/>
    </dbReference>
    <dbReference type="GeneID" id="60825971"/>
    <dbReference type="KEGG" id="rme:Rmet_4559"/>
    <dbReference type="eggNOG" id="COG1522">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_091233_0_3_4"/>
    <dbReference type="OMA" id="TAFILME"/>
    <dbReference type="OrthoDB" id="1534467at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="CDD" id="cd00090">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011991">
        <property type="entry name" value="ArsR-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000485">
        <property type="entry name" value="AsnC-type_HTH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011008">
        <property type="entry name" value="Dimeric_a/b-barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019888">
        <property type="entry name" value="Tscrpt_reg_AsnC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019887">
        <property type="entry name" value="Tscrpt_reg_AsnC/Lrp_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01037">
        <property type="entry name" value="AsnC_trans_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00033">
        <property type="entry name" value="HTHASNC"/>
    </dbReference>
    <dbReference type="SMART" id="SM00344">
        <property type="entry name" value="HTH_ASNC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54909">
        <property type="entry name" value="SSF54909"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50956">
        <property type="entry name" value="HTH_ASNC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0614">Plasmid</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH asnC-type" evidence="4">
        <location>
            <begin position="6"/>
            <end position="67"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50956"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABF11421.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="156" mass="17404" checksum="B989EA23DF9C7C53" modified="2006-05-30" version="1">MLDIELDATDIRILGELQRDGSLTNVELASRVSLSPSPCLARVKRLEKIGVISRRVTLLDARRLGLKVVVFISVSLDKQRRETLDTFERRIAALPQVMECYLMSGDADYLLRVVVPDVEALERLIIDQITRIPGVASIRSSFALKQVLYSTALPLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="95" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LEV2</accession>
    <name>Q1LEV2_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">NADP oxidoreductase, coenzyme F420-dependent:6-phosphogluconate dehydrogenase, NAD-binding</fullName>
            <ecNumber evidence="6">1.1.1.31</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Rmet_4459</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="7">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="7">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EC" id="1.1.1.31" evidence="6"/>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11324.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518927.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4459"/>
    <dbReference type="EnsemblBacteria" id="ABF11324">
        <property type="protein sequence ID" value="ABF11324"/>
        <property type="gene ID" value="Rmet_4459"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4459"/>
    <dbReference type="eggNOG" id="COG2084">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035117_1_0_4"/>
    <dbReference type="OMA" id="IFVDHTT"/>
    <dbReference type="OrthoDB" id="1194694at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008442">
        <property type="term" value="F:3-hydroxyisobutyrate dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016054">
        <property type="term" value="P:organic acid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1040.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002204">
        <property type="entry name" value="3-OH-isobutyrate_DH-rel_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008927">
        <property type="entry name" value="6-PGluconate_DH-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013328">
        <property type="entry name" value="6PGD_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006115">
        <property type="entry name" value="6PGDH_NADP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015815">
        <property type="entry name" value="HIBADH-related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029154">
        <property type="entry name" value="NADP-bd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14833">
        <property type="entry name" value="NAD_binding_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03446">
        <property type="entry name" value="NAD_binding_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000103">
        <property type="entry name" value="HIBADH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48179">
        <property type="entry name" value="SSF48179"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00895">
        <property type="entry name" value="3_HYDROXYISOBUT_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0520">NAD</keyword>
    <keyword evidence="1 6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-0614">Plasmid</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAD_binding_2" evidence="4">
        <location>
            <begin position="3"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="domain" description="NAD_binding_11" evidence="5">
        <location>
            <begin position="168"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="active site" evidence="3">
        <location>
            <position position="174"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR000103-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF03446"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF14833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABF11324.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="291" mass="30808" checksum="2B7DADC2198F8F36" modified="2006-05-30" version="1">MRVAFLGLGVMGYPMAGHLAAKGHDVTVYNRTAAKAERWVQAFGKHGGKQAATPALAAVDCDIVCACVGNDDDLRAVMTGPDGAFQHAAPGTIFVDHTTASASVARELHEAARERGCHFIDAPVSGGQAGAEQGILTIMCGGDPEAFQRAEPVIAAYARAVTRIGESGAGQLAKMVNQISVAGLLQALSEAIAFGEHAGLDMRVVLDVISKGAASSWQMENRGPTMLQSKFDFGFAVDWMRKDLGLCLDEARRNGATLPVTSLVDQFYADLQQMGSSRDDTSSLIKRLRRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-10-07" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LFF2</accession>
    <name>Q1LFF2_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_4258</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11124.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4258"/>
    <dbReference type="EnsemblBacteria" id="ABF11124">
        <property type="protein sequence ID" value="ABF11124"/>
        <property type="gene ID" value="Rmet_4258"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4258"/>
    <dbReference type="HOGENOM" id="CLU_2900989_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="36"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF11124.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="62" mass="6796" checksum="DA2C810FB7C79ADD" modified="2006-05-30" version="1">MSYCDRPSLAASSLFAFFSIGSLLVYAVFGVLYLGAAFAPPIHDHLDGHLLLNRLNVDGRVW</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LG97</accession>
    <name>Q1LG97_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Succinate-semialdehyde dehydrogenase</fullName>
            <ecNumber evidence="3">1.2.1.16</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">gabD</name>
        <name evidence="3" type="ordered locus">Rmet_3961</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
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        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EC" id="1.2.1.16" evidence="3"/>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10829.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518468.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3961"/>
    <dbReference type="EnsemblBacteria" id="ABF10829">
        <property type="protein sequence ID" value="ABF10829"/>
        <property type="gene ID" value="Rmet_3961"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3961"/>
    <dbReference type="eggNOG" id="COG1012">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005391_1_0_4"/>
    <dbReference type="OMA" id="IWRYYAQ"/>
    <dbReference type="OrthoDB" id="384611at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009013">
        <property type="term" value="F:succinate-semialdehyde dehydrogenase [NAD(P)+] activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.309.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.605.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016161">
        <property type="entry name" value="Ald_DH/histidinol_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016163">
        <property type="entry name" value="Ald_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016162">
        <property type="entry name" value="Ald_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015590">
        <property type="entry name" value="Aldehyde_DH_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00171">
        <property type="entry name" value="Aldedh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53720">
        <property type="entry name" value="SSF53720"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aldedh" evidence="2">
        <location>
            <begin position="18"/>
            <end position="471"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF10829.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="475" mass="51543" checksum="8F7278FA3B226F8B" modified="2006-05-30" version="1">MYQDLALYIDGEFIKGGDRREQDVINPATQELLGKLPHATTADLDRALSAAQRAFETWKKTSPLERSQILRRVAELTRTRAKEIGRDITLDQGKPLAEAVGEVMICAEHAEWHAEECRRIYGRVIPPRNPAVRQIVVREPIGVCAAFTPWNFPFNQAIRKIVAALGAGCTLILKGPEDSPSAVVALARLFHDAGLPPGVLNIVWGVPSEVSTYLIESPIVRKISFTGSVPVGKQLAAMAGAHMKRVTMELGGHSPVLVFGDADIEPAAEMLARFKLRNAGQVCVSPTRFYVQEKAYDKFLARFTEVIGSIKVGNGLDDGTQMGPLAHERRIASMEQFLDDASHRGGKIVAGGSRIGDKGFFFAPTVVTDLPDDAKLMVDEPFGPVAPVTRFKDAEEVLRRANSLPFGLASYVFTNSLKTANLVSNGLEAGMVNINHFGMALAETPFGGIKDSGIGSEGGQETFDGYLVTKFITQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LGA8</accession>
    <name>Q1LGA8_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Permease of the major facilitator superfamily</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Rmet_3950</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
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        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="8">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10818.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518458.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3950"/>
    <dbReference type="EnsemblBacteria" id="ABF10818">
        <property type="protein sequence ID" value="ABF10818"/>
        <property type="gene ID" value="Rmet_3950"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3950"/>
    <dbReference type="eggNOG" id="COG0477">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001265_39_0_4"/>
    <dbReference type="OMA" id="PLNRQDY"/>
    <dbReference type="OrthoDB" id="961781at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="SSF103473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0614">Plasmid</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="30"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="70"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="168"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="202"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="256"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="294"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="322"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="348"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="383"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="412"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="domain" description="MFS" evidence="6">
        <location>
            <begin position="29"/>
            <end position="436"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABF10818.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="436" mass="46070" checksum="77C17A2B92040D39" modified="2006-05-30" version="1">MSTSSSTAGASGAQGGHAARPLTGQDYKTLALAALGGALEFYDFIIFVFFAKVIGQLFFPASVPDWLRMLQTFGIFAAGYLARPLGGIIMAHFGDLLGRKKMFTLSILLMSVPTLLMGLLPTYASVGLLAPMALLVLRILQGAAVGGEVPGAWVFVSEHVPRRHVGYACGTLTAGLTAGILLGSLVATGINAVFDPQELVDHGWRVPFVLGGVFGVGSMYLRRWLHETPVFAELQQRKALAAELPLKTVVRDHRGAVVISMLLTWMLSAGIVVVILLTPTYLQTLYGFDARTALVANSAATLCLSIGCVVAGILADRIGARLTLSIGGALLAATACVLYTTIGTRPDLLLPLYALAGFFVGTIGAVPYVLVHAFPAQVRFSGLSFSYNVSYAIFGGLTPVIVSLMLKNDSLAPAHYVVGVCIMGIVTALFVRKRHA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LGI1</accession>
    <name>Q1LGI1_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_3877</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10745.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518392.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="DNASU" id="4040571"/>
    <dbReference type="EnsemblBacteria" id="ABF10745">
        <property type="protein sequence ID" value="ABF10745"/>
        <property type="gene ID" value="Rmet_3877"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3877"/>
    <dbReference type="eggNOG" id="COG3619">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_073333_0_0_4"/>
    <dbReference type="OMA" id="MFRHQGK"/>
    <dbReference type="OrthoDB" id="1751020at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010699">
        <property type="entry name" value="DUF1275"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06912">
        <property type="entry name" value="DUF1275"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="21"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="64"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="100"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
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            <end position="147"/>
        </location>
    </feature>
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        <location>
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            <end position="213"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="219"/>
            <end position="240"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF10745.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="268" mass="29062" checksum="AAE2B7DCB819F13D" modified="2006-05-30" version="1">MPIGFLRSLTARHRNPVSNRRLARVLCFVAGAANAGGFLAVQQYTSHMSGIVSAIADDLVLQRYGLVVHGIGALASFLAGAGTTAILVNWARRARLRSEYAIPLLVEATLLLCFGLLGSHLNDMDWLFVPATVMLLCFMMGLQNALITKLSNAEIRTTHVTGMVTDIGIELGKLFYWNAAVRPNSRPVMADRHKLRLLTVLVGMFLVGGIVGAQAFSTLGYAATLPLAALLLIVAIIPVADDLLTRLLLRVHRADSARRARRWGARGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LGS4</accession>
    <name>Q1LGS4_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Major facilitator superfamily MFS_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Rmet_3782</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="8">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="8">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10652.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518298.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3782"/>
    <dbReference type="EnsemblBacteria" id="ABF10652">
        <property type="protein sequence ID" value="ABF10652"/>
        <property type="gene ID" value="Rmet_3782"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3782"/>
    <dbReference type="eggNOG" id="COG2807">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001265_59_9_4"/>
    <dbReference type="OMA" id="WVLASHQ"/>
    <dbReference type="OrthoDB" id="1594866at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="SSF103473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0614">Plasmid</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="12"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="55"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="87"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="110"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="145"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="175"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="224"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="262"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="288"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="310"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="347"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="376"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="domain" description="MFS" evidence="6">
        <location>
            <begin position="19"/>
            <end position="407"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABF10652.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="415" mass="42920" checksum="A0BBCD5CD4E7F282" modified="2006-05-30" version="1">MHDAPQVLPRAAGWIYVSVILGAALGMLAGYAPLFNATAGVFVRPLAAEFGWGRSQASLSYAASMFGLAVVSPIVGAMMDRFGIRRVIVCSAIVFGLATAAMSLQNGSMLMWVALSVVVGVSGAATSVLGYLAVLPQWFDRRLGLALGIAMCGLGAGTVILPITAQFLVTTFGWRMAYVGLGVGSVVLSLVACGLLRERFSGSRGAKHVPQGVALAAAMRSYRLWAIWLVFVLSSAATLSLGPHLPSLFADRGFDAAMAARSASMVGVGLLVGRLLTGLLIDKIHAPLVASVFFIGGAVGVFLLRGTHDYAALLVATTLIGLTIGAEGDLISYLVRSYFGLTSFGSLFGIAFSGYGLGAVIGPIGLGAWFDRHGSYDVPLLVLPWMLLAAAALVLSLGRYRAGAEGKDATAVASA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LGW7</accession>
    <name>Q1LGW7_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 5">Flagellar hook protein FlgE</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">flgE</name>
        <name evidence="9" type="ordered locus">Rmet_3737</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="10">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="10">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 5">Bacterial flagellum basal body</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the flagella basal body rod proteins family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10609.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518256.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3737"/>
    <dbReference type="EnsemblBacteria" id="ABF10609">
        <property type="protein sequence ID" value="ABF10609"/>
        <property type="gene ID" value="Rmet_3737"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3737"/>
    <dbReference type="eggNOG" id="COG1749">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013687_2_0_4"/>
    <dbReference type="OMA" id="SNVDMSQ"/>
    <dbReference type="OrthoDB" id="704242at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009425">
        <property type="term" value="C:bacterial-type flagellum basal body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.98.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011491">
        <property type="entry name" value="Falgellar_hook_FlgE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037058">
        <property type="entry name" value="Falgellar_hook_FlgE_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001444">
        <property type="entry name" value="Flag_bb_rod_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019776">
        <property type="entry name" value="Flagellar_basal_body_rod_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020013">
        <property type="entry name" value="Flagellar_FlgE/F/G"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010930">
        <property type="entry name" value="Flg_bb/hook_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037925">
        <property type="entry name" value="FlgE/F/G-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07559">
        <property type="entry name" value="FlaE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00460">
        <property type="entry name" value="Flg_bb_rod"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06429">
        <property type="entry name" value="Flg_bbr_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117143">
        <property type="entry name" value="SSF117143"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03506">
        <property type="entry name" value="FlgEFG_subfam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00588">
        <property type="entry name" value="FLAGELLA_BB_ROD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0975">Bacterial flagellum</keyword>
    <keyword evidence="9" id="KW-0966">Cell projection</keyword>
    <keyword evidence="9" id="KW-0969">Cilium</keyword>
    <keyword evidence="9" id="KW-0282">Flagellum</keyword>
    <keyword evidence="9" id="KW-0614">Plasmid</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Flg_bb_rod" evidence="6">
        <location>
            <begin position="5"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="domain" description="FlaE" evidence="8">
        <location>
            <begin position="180"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="domain" description="Flg_bbr_C" evidence="7">
        <location>
            <begin position="339"/>
            <end position="415"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00019015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023143"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU362116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF06429"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF07559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ABF10609.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="415" mass="42505" checksum="95BBDBE1A6C361A5" modified="2006-05-30" version="1">MGFGQGVSGLNAAASNLDVIGNNIANANTVGFKQSTAQFADVYAGSKIGLGTRVNSVVQSFTNGNISSTGRNLDVAISNGNGFFRLASTDGGVYYSRNGQFQRLDDGRLVNASGLQVTGYPAGTTAGGVAPQPIVISNAQMPPQATTNIKAQFQLDSRSTVPTNAFVSTPGATPPVLPNSTMFNYSTAMTVYDSLGNKQQLTAYFGKSIPATTPAPPNAFGGTDWDMNVTDASGNVLQTVKLSFDANGMLKAPSATTMPTVTLPAANGAAAMTATLDLSGTTQFGADNDPKSIVQNGFTSGSLLGFSVQSDGTILGSFSNEQTKSLGQIAMAAFGNAEGLKPEGNNVWSATGASGQELLGVAGGSMGTLQSGSVEESNVDLSGQLVNLIVAQRNYQANAQTIKAQDTVMQTLVNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LH33</accession>
    <name>Q1LH33_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Extra-cytoplasmic Solute Receptor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">bug</name>
        <name evidence="4" type="ordered locus">Rmet_3671</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="5">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="5">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Periplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the UPF0065 (bug) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10543.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518195.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3671"/>
    <dbReference type="EnsemblBacteria" id="ABF10543">
        <property type="protein sequence ID" value="ABF10543"/>
        <property type="gene ID" value="Rmet_3671"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3671"/>
    <dbReference type="eggNOG" id="COG3181">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045683_0_0_4"/>
    <dbReference type="OMA" id="LHHTTRR"/>
    <dbReference type="OrthoDB" id="447734at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042597">
        <property type="term" value="C:periplasmic space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005064">
        <property type="entry name" value="BUG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042100">
        <property type="entry name" value="Bug_dom1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006311">
        <property type="entry name" value="TAT_signal"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42928">
        <property type="entry name" value="PTHR42928"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03401">
        <property type="entry name" value="TctC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017082">
        <property type="entry name" value="YflP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51318">
        <property type="entry name" value="TAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-0675">Receptor</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193449" evidence="3">
        <location>
            <begin position="34"/>
            <end position="334"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF10543.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="334" mass="34795" checksum="2973AF8890386C57" modified="2006-05-30" version="1">MSITKQTSKARRRLLAVGVALATGVAAMTGAQAQGTYPTKPITMIVPFSAGGTTDILARIVGLQLGKALGQPVVIDNRPGAGGNIGASLAAKAPGDGYTLFMGTIGTHAINQSLYSKLPYDPVKDFAPITRVAMVPNIVVVNPKVPVNNIKELIAYVKANPDKLSYGSSGSGSSMHLSGELFNSMTGLHIQHIPYKGSAPAVNDLLGNQIGLMFDNMPSSYPHVKAGKLRAIAVTSAKRSPALPNVPTVAESGVPGYEATSWFALYATGGTPQAVVDRLNAEVVKILAMPEVKKQMAEQGAEPHPEKPAELAAFMKSEAAKWAKVVKASGATVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="95" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LH49</accession>
    <name>Q1LH49_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Pilus assembly protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">cpaE2</name>
        <name evidence="3" type="ordered locus">Rmet_3655</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10527.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3655"/>
    <dbReference type="EnsemblBacteria" id="ABF10527">
        <property type="protein sequence ID" value="ABF10527"/>
        <property type="gene ID" value="Rmet_3655"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3655"/>
    <dbReference type="eggNOG" id="COG4963">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_043017_1_0_4"/>
    <dbReference type="OMA" id="WDRLQIR"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031580">
        <property type="entry name" value="TadZ_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16968">
        <property type="entry name" value="TadZ_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Response regulatory" evidence="2">
        <location>
            <begin position="9"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="1">
        <location>
            <position position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF10527.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="412" mass="44443" checksum="1E6D0DD5B5182CA7" modified="2006-05-30" version="1">MERNTSVDHFLLNTTREDVRHWLGHALGAVGTLVADADSQDSFVEKIGTLRPGLVLLDFTQSRAIDSARLAEQIARLFPQLPLVAVGHAGEPEALLAALRAGVRDFIDLGGAPDHAASVARQLMVPRVQVRPVAATRRDGKIVALLGARPGVGVTSLAVNLAAAVRRGAQAEVLLLDLGLPARDGALYSNVSPEFHFVEAVRNLRRFDQVFVDTAIVRHTNGMSVLPLPASLAEMRDISFSEAMALLDRLREFFDLQVVDLGGFSNAEFMAQIVKAADTVVLVAEQSVGAIVSAAELLQELKKREVERDDLHLLVSRFDAELGVDAEQIADRIGVASVGVLPERRAALLQAGNRGVVLADQSPADPYVRAIGALLERLGFREGQVPERSVLARIKEKLPQVLRPVRAARVGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LHB4</accession>
    <name>Q1LHB4_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Multidrug/solvent efflux outer membrane protein, RND family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Rmet_3590</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the outer membrane factor (OMF) (TC 1.B.17) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10462.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518005.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3590"/>
    <dbReference type="EnsemblBacteria" id="ABF10462">
        <property type="protein sequence ID" value="ABF10462"/>
        <property type="gene ID" value="Rmet_3590"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3590"/>
    <dbReference type="eggNOG" id="COG1538">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012817_13_3_4"/>
    <dbReference type="OMA" id="ILQDRNA"/>
    <dbReference type="OrthoDB" id="1619477at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015562">
        <property type="term" value="F:efflux transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003423">
        <property type="entry name" value="OMP_efflux"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010131">
        <property type="entry name" value="RND_efflux_OM_lipoprot_NodT"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02321">
        <property type="entry name" value="OEP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01845">
        <property type="entry name" value="outer_NodT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0564">Palmitate</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1134">Transmembrane beta strand</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5001442049" evidence="2">
        <location>
            <begin position="22"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="233"/>
            <end position="260"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU362097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF10462.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="491" mass="53620" checksum="91890432CE3C3185" modified="2006-05-30" version="1">MTKTLTTLLLVAGVLSGCTMAPHYDRPAAPVAGGYPAAPEGYASAEVKSGETRRATEIGWREFFTDPRLQGLIEAALENNRDLRTAALRIEEARAQYQIQRADLLPTVNANAGFQRGRFSGASSGIDQSFTGNVYQVGLSVTQWELDFFGRVRSLSNAALAQYLATEEAHRAAYISLVSEVAKAYLAERAFAEQYDLARDTYKARQSTYELAQKRFSAGATSALDLRDNESLVAQARVAAAQLARQRAQAQNALEVLVGKPIAEIPNLPAPMRLSDEHIISDIPEGLPSELLTQRPDIRQAEQQLLSANANIGAARAAFFPRITMTANAGTISTSFTNLFDSGAHVWSFAPQLVLPIFDYGRNMSNLDLSNVRKNIQIANYEKTIQTAFSEVADALVARGTLEDQVAGQEQQRDAEAARYELARMRFRSGVASYLDELDAQRQLFSAEQDLVAARQLRLVNSIDLYRALGGGLVEKGPVAQQPAPQTTTTQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LHD0</accession>
    <name>Q1LHD0_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Starvation-induced protein involved in peptide utilization during carbon starvation</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">cstA</name>
        <name evidence="11" type="ordered locus">Rmet_3574</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptide transporter carbon starvation (CstA) (TC 2.A.114) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10446.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517987.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3574"/>
    <dbReference type="EnsemblBacteria" id="ABF10446">
        <property type="protein sequence ID" value="ABF10446"/>
        <property type="gene ID" value="Rmet_3574"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3574"/>
    <dbReference type="eggNOG" id="COG1966">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010531_2_0_4"/>
    <dbReference type="OMA" id="MLAESFV"/>
    <dbReference type="OrthoDB" id="457782at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009267">
        <property type="term" value="P:cellular response to starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025299">
        <property type="entry name" value="CstA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003706">
        <property type="entry name" value="CstA_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02554">
        <property type="entry name" value="CstA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13722">
        <property type="entry name" value="CstA_5TM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="7"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="33"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="93"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="119"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="160"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="189"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="221"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="255"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="288"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="325"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="365"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="465"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
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            <end position="529"/>
        </location>
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    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="541"/>
            <end position="566"/>
        </location>
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    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
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            <end position="593"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
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        </location>
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        <location>
            <begin position="32"/>
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        </location>
    </feature>
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        <location>
            <begin position="463"/>
            <end position="591"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00007755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF02554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF13722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ABF10446.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="691" mass="74266" checksum="528D84248BBE89AC" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-10-07" version="61" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LHN3</accession>
    <name>Q1LHN3_CUPMC</name>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <lineage>
            <taxon>Bacteria</taxon>
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            <taxon>Betaproteobacteria</taxon>
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        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10343.1"/>
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    </dbReference>
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    <dbReference type="EnsemblBacteria" id="ABF10343">
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    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3471"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193473" evidence="1">
        <location>
            <begin position="27"/>
            <end position="126"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF10343.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="126" mass="14026" checksum="A04405C5F27C4A55" modified="2006-05-30" version="1">MARRLSWLAATATACVVMTMGQSALAQEIPLVTGKQWTQSTEQMKKAYLVGIANLAQVESAYYADKPPGDTQSIMPRLSKGLRGHTLDTVRQRVDNWYAAHPDQLARPVIETIWFDMVIPGLQPQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LHU5</accession>
    <name>Q1LHU5_CUPMC</name>
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        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Rmet_3409</name>
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    <organism evidence="5 6">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
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            <taxon>Bacteria</taxon>
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    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
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                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="CP000352">
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    </dbReference>
    <dbReference type="RefSeq" id="WP_011517845.1">
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    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3409"/>
    <dbReference type="EnsemblBacteria" id="ABF10281">
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        <property type="gene ID" value="Rmet_3409"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3409"/>
    <dbReference type="eggNOG" id="COG3394">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_064244_2_0_4"/>
    <dbReference type="OMA" id="IGRDYGM"/>
    <dbReference type="OrthoDB" id="1204930at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Glyco_hydro/deAcase_b/a-brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017836">
        <property type="entry name" value="Hopanoid_biosynth-assoc_HpnK"/>
    </dbReference>
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        <property type="entry name" value="YdjC-like"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABF10281.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="275" mass="29785" checksum="DB214F66210A4643" modified="2006-05-30" version="1">MTHGQLLIVTADDFGLHHDINEAVERAHCQGILNAASLMVSSPAAADAVERARRLPGLRVGLHLVLADGVATLPYDQIPDLVDASGRFGSAMVRDGFRFFFLPRVRAQLAAEIRAQFEAFAATGLPLDHVNAHKHFHLHPTVLSLILSIGRDYGMRAVRLPREAGGPLLLRPWLALLRARLQRAGIAYNDYVLGLSDTGAMDEATLLAAFEQLPRGVVEMYLHPAVTSGAEVGASMTGYRHADELAALLSPRVRAAADRFAPRRGGFTDLHAGLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LI90</accession>
    <name>Q1LI90_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative transposase or inactive derivative</fullName>
        </submittedName>
    </protein>
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        <name evidence="3" type="ordered locus">Rmet_3264</name>
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        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
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            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
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        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10136.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
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    <dbReference type="EnsemblBacteria" id="ABF10136">
        <property type="protein sequence ID" value="ABF10136"/>
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    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3264"/>
    <dbReference type="eggNOG" id="COG1943">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_068226_1_2_4"/>
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    <dbReference type="OrthoDB" id="1547701at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
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        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="F:transposase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002686">
        <property type="entry name" value="Transposase_17"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036515">
        <property type="entry name" value="Transposase_17_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01797">
        <property type="entry name" value="Y1_Tnp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01321">
        <property type="entry name" value="Y1_Tnp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143422">
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Y1_Tnp" evidence="2">
        <location>
            <begin position="9"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="214"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="223"/>
            <end position="241"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM01321"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF10136.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="247" mass="27646" checksum="91DBE12E3FF792B4" modified="2006-05-30" version="1">MARLPRFSPAGLPALVLQRGNNRQPVFLGPDDYLHYLDCLRMAAREHDVAVHAYVLRPNHIHLVATPKGADSLSLTMQAVGRRYARHFNRAASRTGTLWEGRFRSAVIEPGAWLLPAMLYVESNAMRAGDTATLEADRWSSYRHHIGIEASPLVSDHSIYWELGNTPFERQSNYRALALEGLSGKTLATLQKHAHSGWPLGDDAFLAQLERHATRRIHPLPKGRPRKSTESESERPGDTEDFDSAPT</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LI94</accession>
    <name>Q1LI94_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Extra-cytoplasmic Solute Receptor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">bug</name>
        <name evidence="3" type="ordered locus">Rmet_3260</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Periplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the UPF0065 (bug) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10132.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517732.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3260"/>
    <dbReference type="EnsemblBacteria" id="ABF10132">
        <property type="protein sequence ID" value="ABF10132"/>
        <property type="gene ID" value="Rmet_3260"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3260"/>
    <dbReference type="eggNOG" id="COG3181">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045683_0_2_4"/>
    <dbReference type="OMA" id="RSESIQW"/>
    <dbReference type="OrthoDB" id="447734at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042597">
        <property type="term" value="C:periplasmic space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005064">
        <property type="entry name" value="BUG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042100">
        <property type="entry name" value="Bug_dom1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42928">
        <property type="entry name" value="PTHR42928"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03401">
        <property type="entry name" value="TctC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017082">
        <property type="entry name" value="YflP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0675">Receptor</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF10132.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="372" mass="39497" checksum="DE31360A985BB75D" modified="2006-05-30" version="1">MSEVRKSRRLWRGIAKLPKLAAILAVLRCGPRSAISWGLVACAASTATALAQPPAIAQLASIPSAGRSEMSRPVSFPARPIRMIVPFPAGGVSDAVARLLAERLSARLGVPIEVDNRPGASGTMAGDVVAKASSDGHTLLFHQANILIQPGLEPVPYDVIRDFVPVGRVATMPLFLVIDGRLPMQTPQQWATAVRSNPGSYSYGYGQPGSPSHLYAEYAVRGLPNAVPLVTAKGESAVVQEMLAGRISACFCSYVSIQQHIRSGALRMIAVTGVARSPLAPQVPTLQESGIEGYAAAAWYGVMVPAKTPRAIVARLANELDNVTEEREVRARMLAAGLTPMQDSPEAFATAIRSESTQWQAILRQTGQHGDP</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="59" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LIF9</accession>
    <name>Q1LIF9_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_3195</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10067.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517676.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3195"/>
    <dbReference type="EnsemblBacteria" id="ABF10067">
        <property type="protein sequence ID" value="ABF10067"/>
        <property type="gene ID" value="Rmet_3195"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3195"/>
    <dbReference type="eggNOG" id="COG4929">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_105410_0_0_4"/>
    <dbReference type="OMA" id="SIMQGDY"/>
    <dbReference type="OrthoDB" id="1812417at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025833">
        <property type="entry name" value="GDYXXLXY"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14345">
        <property type="entry name" value="GDYXXLXY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF10067.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="166" mass="18566" checksum="393B92768D9C6B65" modified="2006-05-30" version="1">MKRWIMIGWLLTIAVAVAGIVGKERVISRGDVVYLRLAPVDPRSLMQGDYMALNFAIGMEVSAAHGQDQTENRRDELVVIRRDASNEGHFVRLHKGEALAAGEQLLRVQNVASRWGRSNVQVSTDAWFFQEGHAERFSKARFGEFRVDASGQALLVGMRDDKLAPM</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="64" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LII0</accession>
    <name>Q1LII0_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_3174</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10046.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517658.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF10046">
        <property type="protein sequence ID" value="ABF10046"/>
        <property type="gene ID" value="Rmet_3174"/>
    </dbReference>
    <dbReference type="GeneID" id="60825467"/>
    <dbReference type="KEGG" id="rme:Rmet_3174"/>
    <dbReference type="HOGENOM" id="CLU_186128_0_0_4"/>
    <dbReference type="OMA" id="HPSRANQ"/>
    <dbReference type="OrthoDB" id="1955893at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="37"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="47"/>
            <end position="65"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF10046.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="65" mass="7115" checksum="01C3C557B29438B4" modified="2006-05-30" version="1">MSQFFENHPTLLLAAEAGVALLLLIFIVVWTMSTAKKNPRPTRAPQDHPSRANQTSTDADTPPEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LIS8</accession>
    <name>Q1LIS8_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Extra-cytoplasmic Solute Receptor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">bug</name>
        <name evidence="4" type="ordered locus">Rmet_3076</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Periplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the UPF0065 (bug) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09948.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517577.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3076"/>
    <dbReference type="EnsemblBacteria" id="ABF09948">
        <property type="protein sequence ID" value="ABF09948"/>
        <property type="gene ID" value="Rmet_3076"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3076"/>
    <dbReference type="eggNOG" id="COG3181">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045683_0_0_4"/>
    <dbReference type="OMA" id="QRISAYP"/>
    <dbReference type="OrthoDB" id="447734at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042597">
        <property type="term" value="C:periplasmic space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005064">
        <property type="entry name" value="BUG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042100">
        <property type="entry name" value="Bug_dom1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42928">
        <property type="entry name" value="PTHR42928"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03401">
        <property type="entry name" value="TctC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017082">
        <property type="entry name" value="YflP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0675">Receptor</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193592" evidence="3">
        <location>
            <begin position="27"/>
            <end position="323"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF09948.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="323" mass="33548" checksum="D1216670187978B0" modified="2006-05-30" version="1">MRFSPRGRVMALALATTSLIAATAHAADAWPNHVIKFVVPFTAGGANDLVARAGAEAVSKRIGQPVVIENRPGAGGIVGADYVAKSKPDGYTFLVGAVGTVTNGLIRSKMPYAPDDLVPVGLIAVSPSVIVASPSVPVNNVKELIEYSKKQGGVPFATAGSGSTPHFVEEMLKEKGAVLTVVPYKSGSESITAVIGNQVVATSEASIVVLPQIKAGKLKALGATWDKRMPAAPNIATTAEQGLPEVRIGHWAGIFAPKGTDPAIIKKLNAELSAGMQSPDIRERLIQNGIEPAGGSVESFVAFIKSERERLGRIAKNANMSAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="69" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LJE4</accession>
    <name>Q1LJE4_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Taurine ABC-type transporter, periplasmic component</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">tauA</name>
        <name evidence="4" type="ordered locus">Rmet_2859</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09732.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517416.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2859"/>
    <dbReference type="EnsemblBacteria" id="ABF09732">
        <property type="protein sequence ID" value="ABF09732"/>
        <property type="gene ID" value="Rmet_2859"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2859"/>
    <dbReference type="eggNOG" id="COG0715">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028871_3_2_4"/>
    <dbReference type="OMA" id="AMTYEPY"/>
    <dbReference type="OrthoDB" id="785019at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001638">
        <property type="entry name" value="Solute-binding_3/MltF_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015168">
        <property type="entry name" value="SsuA/THI5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09084">
        <property type="entry name" value="NMT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00062">
        <property type="entry name" value="PBPb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193607" evidence="2">
        <location>
            <begin position="25"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="domain" description="PBPb" evidence="3">
        <location>
            <begin position="28"/>
            <end position="274"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00062"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF09732.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="317" mass="33864" checksum="1A9D43E94A7A17CB" modified="2006-05-30" version="1">MSLQRHAVTAILALAASAAATAHADNTPVVLGMSGWTGFAPLTLADKAGIFKKHGVDVDIKMIPQKDRHLALASGAIQCAATTVETHVAWNANGVPITQIVQLDKSYGADGLAVRSDVKSFADLKGKTIGVDAPGTAPYFGLAWMLKKNGMTLKDVKVTTLSPQAAAQAFVAGQNDGAMTYEPYLSTVRANPDKGKILATTLDYPMVTDTLGCAPKWLKDNPKAAQALVDSYFEALEMIRKEPDKANEIMGAAVKQTGEQFAKSAAYLRWQDKDANRKFFSGELASFSKEAAGVLMDIGVIRQVPDVTAIYDARFLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="95" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LJM4</accession>
    <name>Q1LJM4_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Bifunctional adenosylcobalamin biosynthesis protein</fullName>
            <ecNumber evidence="12">2.7.1.156</ecNumber>
            <ecNumber evidence="12">2.7.7.62</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">cobU</name>
        <name evidence="16" type="ordered locus">Rmet_2779</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4 12">Catalyzes ATP-dependent phosphorylation of adenosylcobinamide and addition of GMP to adenosylcobinamide phosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 12">
            <text>adenosylcob(III)inamide + ATP = adenosylcob(III)inamide phosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:15769"/>
            <dbReference type="ChEBI" id="CHEBI:2480"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58502"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.156"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>adenosylcob(III)inamide + GTP = adenosylcob(III)inamide phosphate + GDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:15765"/>
            <dbReference type="ChEBI" id="CHEBI:2480"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="ChEBI" id="CHEBI:58502"/>
            <dbReference type="EC" id="2.7.1.156"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2 12">
            <text>adenosylcob(III)inamide phosphate + GTP + H(+) = adenosylcob(III)inamide-GDP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:22712"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:58502"/>
            <dbReference type="ChEBI" id="CHEBI:60487"/>
            <dbReference type="EC" id="2.7.7.62"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="6 12">Cofactor biosynthesis; adenosylcobalamin biosynthesis; adenosylcobalamin from cob(II)yrinate a,c-diamide: step 5/7.</text>
    </comment>
    <comment type="pathway">
        <text evidence="5 12">Cofactor biosynthesis; adenosylcobalamin biosynthesis; adenosylcobalamin from cob(II)yrinate a,c-diamide: step 6/7.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7 12">Belongs to the CobU/CobP family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.156" evidence="12"/>
    <dbReference type="EC" id="2.7.7.62" evidence="12"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09652.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2779"/>
    <dbReference type="EnsemblBacteria" id="ABF09652">
        <property type="protein sequence ID" value="ABF09652"/>
        <property type="gene ID" value="Rmet_2779"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2779"/>
    <dbReference type="eggNOG" id="COG2087">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_094161_0_1_4"/>
    <dbReference type="OMA" id="DCLTVWL"/>
    <dbReference type="UniPathway" id="UPA00148">
        <property type="reaction ID" value="UER00236"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036429">
        <property type="term" value="F:adenosylcobinamide kinase (ATP-specific) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036428">
        <property type="term" value="F:adenosylcobinamide kinase (GTP-specific) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008820">
        <property type="term" value="F:cobinamide phosphate guanylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009236">
        <property type="term" value="P:cobalamin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00544">
        <property type="entry name" value="CobU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003203">
        <property type="entry name" value="Cobinamide_kinase/P_G-Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34848">
        <property type="entry name" value="PTHR34848"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02283">
        <property type="entry name" value="CobU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006135">
        <property type="entry name" value="CobU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="12" id="KW-0169">Cobalamin biosynthesis</keyword>
    <keyword evidence="12 14" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="10 12 16" id="KW-0418">Kinase</keyword>
    <keyword evidence="9 12 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 12 16" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="active site" description="GMP-histidine intermediate" evidence="13">
        <location>
            <position position="77"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <begin position="28"/>
            <end position="35"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <begin position="57"/>
            <end position="59"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <begin position="78"/>
            <end position="81"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="89"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="111"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000312"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00000711"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00001522"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00003889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00005159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00007490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRNR" id="PIRNR006135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR006135-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR006135-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="ABF09652.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="214" mass="23314" checksum="CB80941BD3C6345C" modified="2006-05-30" version="1">MAGEPEARPTHEPHASMSMPRQLTLVIGGARSGKSHFAEQLATDHAALTRGPVTYIATARHDVDSTDEEMELRIALHRARRPADWTLVEEPVHLADALYGHARHDGCILVDCVTLWLNNLIFSESRDYPEHGVITPPPAFTEEIDALMTALPMLPGHVILVTNEIGFGVVPMGAITRFYVDELGRLNQKLAAAADRVRLLVAGIPVAVKDAPIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LJQ7</accession>
    <name>Q1LJQ7_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative Acyltransferase 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_2746</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09619.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517319.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2746"/>
    <dbReference type="EnsemblBacteria" id="ABF09619">
        <property type="protein sequence ID" value="ABF09619"/>
        <property type="gene ID" value="Rmet_2746"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2746"/>
    <dbReference type="eggNOG" id="COG1835">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005679_3_0_4"/>
    <dbReference type="OMA" id="LAYEWRF"/>
    <dbReference type="OrthoDB" id="1979131at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016747">
        <property type="term" value="F:acyltransferase activity, transferring groups other than amino-acyl groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002656">
        <property type="entry name" value="Acyl_transf_3_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01757">
        <property type="entry name" value="Acyl_transf_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="46"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="77"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="119"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="180"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="209"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="287"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="320"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="350"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl_transf_3" evidence="2">
        <location>
            <begin position="43"/>
            <end position="366"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01757"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF09619.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="408" mass="44864" checksum="7C234D737C0B7750" modified="2006-05-30" version="1">MDLYSIWPSAAVIALCLALVWPKRLWRFLDDPPSGQGQRYVTVDGLRGFLALAVVLHHCVISYGFHQTGEWKLPPSSFYSIIGQVGVSIFFMITAFLFWGRLLDQGKRLDWFALYVNRFLRIAPLYWVVVALMLLVVAIKTGFTLAVPPSELVKQVFQWALPAIVRGMPPVNGYQQTSTITAGVTWTLYYEWMFYFSLPFLAIAAIKRSPLAFVPACIWIVFVMPETLSSAFARNLVAMFVMGMVAASLVRRSPGFRGDSVLKSAIAVALLAFPLLTRSTAYESVSILSLGAFFILVSSGASLFGLLASRSAVRLGSVSYGIYLLQGIVITVLHSPRVLGAFACKGPEQFWLTTIAVGLVLVCVAAASYHFVERPCIRLGKKIGKAPESARQTHVEMGDGTPSDKARC</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-10-07" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LK38</accession>
    <name>Q1LK38_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_2611</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09488.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2611"/>
    <dbReference type="EnsemblBacteria" id="ABF09488">
        <property type="protein sequence ID" value="ABF09488"/>
        <property type="gene ID" value="Rmet_2611"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2611"/>
    <dbReference type="HOGENOM" id="CLU_2357666_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF09488.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="96" mass="11024" checksum="FFB2F84393FB9682" modified="2006-05-30" version="1">MTKPGWPSTTPRPACSRTRCTRRSPCIANTAAWCPNSHHATISGACCRYCSRYWTMQAARARTSTPSLTRRAPDLPARCWWAHRWPMRWASRSVCR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-01-15" modified="2022-05-25" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LKL8</accession>
    <name>PLSX_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Phosphate acyltransferase</fullName>
            <ecNumber evidence="1">2.3.1.274</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Acyl-ACP phosphotransacylase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Acyl-[acyl-carrier-protein]--phosphate acyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Phosphate-acyl-ACP acyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">plsX</name>
        <name type="ordered locus">Rmet_2431</name>
    </gene>
    <organism>
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the reversible formation of acyl-phosphate (acyl-PO(4)) from acyl-[acyl-carrier-protein] (acyl-ACP). This enzyme utilizes acyl-ACP as fatty acyl donor, but not acyl-CoA.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a fatty acyl-[ACP] + phosphate = an acyl phosphate + holo-[ACP]</text>
            <dbReference type="Rhea" id="RHEA:42292"/>
            <dbReference type="Rhea" id="RHEA-COMP:9685"/>
            <dbReference type="Rhea" id="RHEA-COMP:14125"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:59918"/>
            <dbReference type="ChEBI" id="CHEBI:64479"/>
            <dbReference type="ChEBI" id="CHEBI:138651"/>
            <dbReference type="EC" id="2.3.1.274"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Lipid metabolism; phospholipid metabolism.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer. Probably interacts with PlsY.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
        <text evidence="1">Associated with the membrane possibly through PlsY.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the PlsX family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.274" evidence="1"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09308.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517025.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q1LKL8"/>
    <dbReference type="SMR" id="Q1LKL8"/>
    <dbReference type="STRING" id="266264.Rmet_2431"/>
    <dbReference type="EnsemblBacteria" id="ABF09308">
        <property type="protein sequence ID" value="ABF09308"/>
        <property type="gene ID" value="Rmet_2431"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2431"/>
    <dbReference type="eggNOG" id="COG0416">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039379_1_0_4"/>
    <dbReference type="OMA" id="HGKSNAR"/>
    <dbReference type="OrthoDB" id="631784at2"/>
    <dbReference type="UniPathway" id="UPA00085"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043811">
        <property type="term" value="F:phosphate:acyl-[acyl carrier protein] acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006633">
        <property type="term" value="P:fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008654">
        <property type="term" value="P:phospholipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00019">
        <property type="entry name" value="PlsX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003664">
        <property type="entry name" value="FA_synthesis"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012281">
        <property type="entry name" value="Phospholipid_synth_PlsX-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30100">
        <property type="entry name" value="PTHR30100"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02504">
        <property type="entry name" value="FA_synthesis"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002465">
        <property type="entry name" value="Phsphlp_syn_PlsX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00182">
        <property type="entry name" value="plsX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0444">Lipid biosynthesis</keyword>
    <keyword id="KW-0443">Lipid metabolism</keyword>
    <keyword id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword id="KW-1208">Phospholipid metabolism</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_1000001809" description="Phosphate acyltransferase">
        <location>
            <begin position="1"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="335"/>
            <end position="359"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="359" mass="37937" checksum="844D7AEAD36A589B" modified="2006-05-30" version="1">MTIKLAIDCMGGDHGVSVTVPAAISFLSSHDDAEMILVGLPDAIGAQLKKLHATDHPRVTVVPATEVITMDDPVEVALRKKKDSSMRVAATQVKEGKADACISAGNTGALMAVSRYVLKTLEGIERPAIATTIPNEQGWGTTVLDLGANADCEPGHLLQFARMADAMVSVVDHKEHPTVGLLNIGEEVIKGNEVVKAAGELLRASELNFYGNVEGNDIFKGTTDIVVCDGFVGNVALKSTEGLAKMIGSMIKEEFTRSWFTKLLAVVAMPVLSRLARRLDPARYNGASLLGLRGLVIKSHGSADAHSFEWAIKRGYDAARNGVIERIARAFAHKSGAGGAATGSPETDAPNPHPDSRAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="55" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LKY8</accession>
    <name>Q1LKY8_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_2309</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09188.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_003116829.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2309"/>
    <dbReference type="EnsemblBacteria" id="ABF09188">
        <property type="protein sequence ID" value="ABF09188"/>
        <property type="gene ID" value="Rmet_2309"/>
    </dbReference>
    <dbReference type="GeneID" id="61526435"/>
    <dbReference type="KEGG" id="rme:Rmet_2309"/>
    <dbReference type="eggNOG" id="ENOG502Z8H7">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_112900_0_0_4"/>
    <dbReference type="OMA" id="FYSRAWE"/>
    <dbReference type="OrthoDB" id="1511258at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF09188.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="245" mass="26587" checksum="9A4F036BF7A1D9C1" modified="2006-05-30" version="1">MSQNPNPFLRGYWNLKIVRTLSISYEDGSPHVWRNIHPSQQHLCDAALVSSPCIITSDFAVVRTGTEPVGAALIAECDAAEGGSGEGMVGAVVYAIHGDDFDGRPVHIGDTYSAEAAREVVQRLSFETGYYSRCWEISSAHISRETGQYLANLADLATPEAFLFIAFRIPYSPAIGVKLISTPWTDQHLQDVEGIAAEQLRQEHRSKGMPDELAQILELAGQADVRILILDADAPVLPGLSLAGE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-04-14" modified="2022-08-03" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LL91</accession>
    <name>GLO2_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Hydroxyacylglutathione hydrolase</fullName>
            <ecNumber evidence="1">3.1.2.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Glyoxalase II</fullName>
            <shortName evidence="1">Glx II</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">gloB</name>
        <name type="ordered locus">Rmet_2206</name>
    </gene>
    <organism>
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Thiolesterase that catalyzes the hydrolysis of S-D-lactoyl-glutathione to form glutathione and D-lactic acid.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>an S-(2-hydroxyacyl)glutathione + H2O = a 2-hydroxy carboxylate + glutathione + H(+)</text>
            <dbReference type="Rhea" id="RHEA:21864"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57925"/>
            <dbReference type="ChEBI" id="CHEBI:58896"/>
            <dbReference type="ChEBI" id="CHEBI:71261"/>
            <dbReference type="EC" id="3.1.2.6"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="1">Binds 2 Zn(2+) ions per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Secondary metabolite metabolism; methylglyoxal degradation; (R)-lactate from methylglyoxal: step 2/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Monomer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the metallo-beta-lactamase superfamily. Glyoxalase II family.</text>
    </comment>
    <dbReference type="EC" id="3.1.2.6" evidence="1"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09085.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516913.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q1LL91"/>
    <dbReference type="SMR" id="Q1LL91"/>
    <dbReference type="STRING" id="266264.Rmet_2206"/>
    <dbReference type="EnsemblBacteria" id="ABF09085">
        <property type="protein sequence ID" value="ABF09085"/>
        <property type="gene ID" value="Rmet_2206"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2206"/>
    <dbReference type="eggNOG" id="COG0491">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030571_4_1_4"/>
    <dbReference type="OMA" id="NYIWLLQ"/>
    <dbReference type="OrthoDB" id="733552at2"/>
    <dbReference type="UniPathway" id="UPA00619">
        <property type="reaction ID" value="UER00676"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004416">
        <property type="term" value="F:hydroxyacylglutathione hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019243">
        <property type="term" value="P:methylglyoxal catabolic process to D-lactate via S-lactoyl-glutathione"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07723">
        <property type="entry name" value="hydroxyacylglutathione_hydrolase_MBL-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01374">
        <property type="entry name" value="Glyoxalase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035680">
        <property type="entry name" value="Clx_II_MBL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032282">
        <property type="entry name" value="HAGH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017782">
        <property type="entry name" value="Hydroxyacylglutathione_Hdrlase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001279">
        <property type="entry name" value="Metallo-B-lactamas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16123">
        <property type="entry name" value="HAGH_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00753">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005457">
        <property type="entry name" value="Glx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00849">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="SSF56281"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03413">
        <property type="entry name" value="GSH_gloB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <feature type="chain" id="PRO_1000144793" description="Hydroxyacylglutathione hydrolase">
        <location>
            <begin position="1"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="53"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="55"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="57"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="58"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="118"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="140"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="140"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="178"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01374"/>
        </source>
    </evidence>
    <sequence length="266" mass="28137" checksum="435AA549216FF9EC" modified="2006-05-30" version="1">MLKVEPIPAFQDNYIWAIHDGHSAAVVDPGEAAPVEQFLAQRGLALGAIVITHHHGDHQGGVSDLLARHPSGPNGEPLPVIGPQGERIGHRTQAVREGDTVTLRHPAVTFRVLDVPGHTAGHVAYVGDLPGAGPVVFCGDTLFASGCGRLFEGTPAQMLASLDKLAALPGDTRVYCAHEYTRSNVRFAQAVEPANPDLAAWAARVDILREAGTPTVPTTVAHERAVNPFLRSRESTVRQAVRAQGGDVSGDAAAFGALRGWKDGFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LLK3</accession>
    <name>Q1LLK3_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">NAD-dependent epimerase/dehydratase</fullName>
            <ecNumber evidence="2">1.5.1.30</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_2094</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EC" id="1.5.1.30" evidence="2"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08973.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516807.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2094"/>
    <dbReference type="EnsemblBacteria" id="ABF08973">
        <property type="protein sequence ID" value="ABF08973"/>
        <property type="gene ID" value="Rmet_2094"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2094"/>
    <dbReference type="eggNOG" id="COG2910">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025711_3_1_4"/>
    <dbReference type="OMA" id="DWTFLSP"/>
    <dbReference type="OrthoDB" id="948454at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042602">
        <property type="term" value="F:riboflavin reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016040">
        <property type="entry name" value="NAD(P)-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13460">
        <property type="entry name" value="NAD_binding_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAD(P)-bd_dom" evidence="1">
        <location>
            <begin position="7"/>
            <end position="192"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF08973.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="203" mass="21806" checksum="678EED28FDABE78A" modified="2006-05-30" version="1">MKIAIIGATGNVGTRLTDEALRRGHTVTAIARQASKLPTRDGVTTRDADVADAKALADAVRGNDVVISTVRFLQATAAQITDAAKTAGIKRLLVVGGAGSLYVAPGVQLIDTPEFPDLYKAEASAGRDFLNALRSESQLEWTFLSPSALFTPGERTGKFRLGKEDLLVAADGKSWISMEDYAIAMLDEIEQPKHVRERFTVGY</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="109" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LLK5</accession>
    <name>Q1LLK5_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Ribonuclease R</fullName>
            <shortName evidence="6">RNase R</shortName>
            <ecNumber evidence="6">3.1.13.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6 9" type="primary">rnr</name>
        <name evidence="9" type="ordered locus">Rmet_2092</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">3'-5' exoribonuclease that releases 5'-nucleoside monophosphates and is involved in maturation of structured RNAs.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 6">
            <text>Exonucleolytic cleavage in the 3'- to 5'-direction to yield nucleoside 5'-phosphates.</text>
            <dbReference type="EC" id="3.1.13.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the RNR ribonuclease family. RNase R subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.13.1" evidence="6"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08971.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516805.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2092"/>
    <dbReference type="EnsemblBacteria" id="ABF08971">
        <property type="protein sequence ID" value="ABF08971"/>
        <property type="gene ID" value="Rmet_2092"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2092"/>
    <dbReference type="eggNOG" id="COG0557">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002333_7_0_4"/>
    <dbReference type="OMA" id="DWYEYRS"/>
    <dbReference type="OrthoDB" id="1602988at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008859">
        <property type="term" value="F:exoribonuclease II activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01895">
        <property type="entry name" value="RNase_R"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011129">
        <property type="entry name" value="CSD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040476">
        <property type="entry name" value="CSD2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013223">
        <property type="entry name" value="RNase_B_OB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001900">
        <property type="entry name" value="RNase_II/R"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022966">
        <property type="entry name" value="RNase_II/R_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004476">
        <property type="entry name" value="RNase_II/RNase_R"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011805">
        <property type="entry name" value="RNase_R"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022967">
        <property type="entry name" value="S1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003029">
        <property type="entry name" value="S1_domain"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17876">
        <property type="entry name" value="CSD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08206">
        <property type="entry name" value="OB_RNB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00773">
        <property type="entry name" value="RNB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00575">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00357">
        <property type="entry name" value="CSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00955">
        <property type="entry name" value="RNB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00316">
        <property type="entry name" value="S1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00358">
        <property type="entry name" value="3_prime_RNase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02063">
        <property type="entry name" value="RNase_R"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01175">
        <property type="entry name" value="RIBONUCLEASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50126">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 6" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="4 6 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 6" id="KW-0540">Nuclease</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="S1 motif" evidence="8">
        <location>
            <begin position="663"/>
            <end position="744"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="811"/>
            <end position="876"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="847"/>
            <end position="861"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ABF08971.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="876" mass="98178" checksum="2CD0B424F1BA9AA9" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="69" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LLP1</accession>
    <name>Q1LLP1_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Hydrolase (HAD superfamily IIB)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_2056</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08935.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516767.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2056"/>
    <dbReference type="EnsemblBacteria" id="ABF08935">
        <property type="protein sequence ID" value="ABF08935"/>
        <property type="gene ID" value="Rmet_2056"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2056"/>
    <dbReference type="eggNOG" id="COG0561">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_075026_0_0_4"/>
    <dbReference type="OMA" id="VQCHIKP"/>
    <dbReference type="OrthoDB" id="1861282at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016791">
        <property type="term" value="F:phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006379">
        <property type="entry name" value="HAD-SF_hydro_IIB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="SSF56784"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01484">
        <property type="entry name" value="HAD-SF-IIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF08935.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="268" mass="29145" checksum="5C7757929603F031" modified="2006-05-30" version="1">MTSPQPFSALPDAALRRVRGILTDIDGTLTTDGRLPAATYLALDRLTRAGLHVIPVTGRCIAWAEILTRLWPVDAIIGENGAFYSHLRDGKLVTRFLDDAETRDANLFRIHALGQEILREAPGCALASDQAWHAADLAIDHAEDVPPLPQPAVDRIAEIMREAGMTATISSIHVNGWFGHHDKLSMSRLCVEELLGDDVDAHRDEWLFIGDSANDVAMFGHFPLSVGVANVGDILHLLPHKPAYVTNASGGEGFAEMAERLLGLRQLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LLR2</accession>
    <name>Q1LLR2_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Putative transcriptional regulator (ScpB/YpuH-like)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Rmet_2035</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08914.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516749.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2035"/>
    <dbReference type="EnsemblBacteria" id="ABF08914">
        <property type="protein sequence ID" value="ABF08914"/>
        <property type="gene ID" value="Rmet_2035"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2035"/>
    <dbReference type="eggNOG" id="COG1386">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045647_2_4_4"/>
    <dbReference type="OMA" id="LICAREP"/>
    <dbReference type="OrthoDB" id="1771906at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051304">
        <property type="term" value="P:chromosome separation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005234">
        <property type="entry name" value="ScpB_csome_segregation"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34298">
        <property type="entry name" value="PTHR34298"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04079">
        <property type="entry name" value="SMC_ScpB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00281">
        <property type="entry name" value="TIGR00281"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="1" id="KW-0132">Cell division</keyword>
    <keyword evidence="2" id="KW-0159">Chromosome partition</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="214"/>
            <end position="302"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABF08914.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="302" mass="32872" checksum="89CFB522CC25D7BD" modified="2006-05-30" version="1">MNTQEAKIVLETALICAQEPLRVGDLRRLFADDVGADTIRVLLEELRNDWQQRGVELVALASGWRFQSRPEMREFLDRLNPEKPPKYSRAVMETLAIIAYRQPVTRGDIEEIRGVTVSTEVVKKLEDRDWIEVIGHRDVPGRPALYATTKAFLDDLGLRSLDELPPLEDAQAQAQAALLDQQAIDFEAVATANPPAGDEEAFSEATVGVAETGEPAVESLSVEPQSEDLSGGASDAAGNENEQNDSVAQLPEGDGHDDDAPVAQANGFHVDTESSSQEDAAVPARDGDMPLTTEDDDERVSN</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-01-15" modified="2022-08-03" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LLZ4</accession>
    <name>ISPF_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase</fullName>
            <shortName evidence="1">MECDP-synthase</shortName>
            <shortName evidence="1">MECPP-synthase</shortName>
            <shortName evidence="1">MECPS</shortName>
            <ecNumber evidence="1">4.6.1.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">ispF</name>
        <name type="ordered locus">Rmet_1953</name>
    </gene>
    <organism>
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in the biosynthesis of isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP), two major building blocks of isoprenoid compounds. Catalyzes the conversion of 4-diphosphocytidyl-2-C-methyl-D-erythritol 2-phosphate (CDP-ME2P) to 2-C-methyl-D-erythritol 2,4-cyclodiphosphate (ME-CPP) with a corresponding release of cytidine 5-monophosphate (CMP).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>4-CDP-2-C-methyl-D-erythritol 2-phosphate = 2-C-methyl-D-erythritol 2,4-cyclic diphosphate + CMP</text>
            <dbReference type="Rhea" id="RHEA:23864"/>
            <dbReference type="ChEBI" id="CHEBI:57919"/>
            <dbReference type="ChEBI" id="CHEBI:58483"/>
            <dbReference type="ChEBI" id="CHEBI:60377"/>
            <dbReference type="EC" id="4.6.1.12"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
        <text evidence="1">Binds 1 divalent metal cation per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 4/6.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homotrimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the IspF family.</text>
    </comment>
    <dbReference type="EC" id="4.6.1.12" evidence="1"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08832.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516680.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q1LLZ4"/>
    <dbReference type="SMR" id="Q1LLZ4"/>
    <dbReference type="STRING" id="266264.Rmet_1953"/>
    <dbReference type="EnsemblBacteria" id="ABF08832">
        <property type="protein sequence ID" value="ABF08832"/>
        <property type="gene ID" value="Rmet_1953"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1953"/>
    <dbReference type="eggNOG" id="COG0245">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_084630_2_0_4"/>
    <dbReference type="OMA" id="QHFGTGR"/>
    <dbReference type="OrthoDB" id="1716560at2"/>
    <dbReference type="UniPathway" id="UPA00056">
        <property type="reaction ID" value="UER00095"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008685">
        <property type="term" value="F:2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019288">
        <property type="term" value="P:isopentenyl diphosphate biosynthetic process, methylerythritol 4-phosphate pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016114">
        <property type="term" value="P:terpenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00554">
        <property type="entry name" value="MECDP_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00107">
        <property type="entry name" value="IspF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003526">
        <property type="entry name" value="MECDP_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020555">
        <property type="entry name" value="MECDP_synthase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036571">
        <property type="entry name" value="MECDP_synthase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43181">
        <property type="entry name" value="PTHR43181"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02542">
        <property type="entry name" value="YgbB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69765">
        <property type="entry name" value="SSF69765"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00151">
        <property type="entry name" value="ispF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01350">
        <property type="entry name" value="ISPF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0414">Isoprene biosynthesis</keyword>
    <keyword id="KW-0456">Lyase</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_1000022868" description="2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase">
        <location>
            <begin position="1"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="13"/>
            <end position="15"/>
        </location>
        <ligand>
            <name>4-CDP-2-C-methyl-D-erythritol 2-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:57919"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="13"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="15"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="39"/>
            <end position="40"/>
        </location>
        <ligand>
            <name>4-CDP-2-C-methyl-D-erythritol 2-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:57919"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="47"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="61"/>
            <end position="63"/>
        </location>
        <ligand>
            <name>4-CDP-2-C-methyl-D-erythritol 2-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:57919"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="66"/>
            <end position="70"/>
        </location>
        <ligand>
            <name>4-CDP-2-C-methyl-D-erythritol 2-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:57919"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="144"/>
        </location>
        <ligand>
            <name>4-CDP-2-C-methyl-D-erythritol 2-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:57919"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="147"/>
        </location>
        <ligand>
            <name>4-CDP-2-C-methyl-D-erythritol 2-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:57919"/>
        </ligand>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="1">
        <location>
            <position position="39"/>
        </location>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="1">
        <location>
            <position position="138"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00107"/>
        </source>
    </evidence>
    <sequence length="168" mass="17908" checksum="C1F60B02EBC78760" modified="2006-05-30" version="1">MNPFDIRIGQGYDVHALVPGRKLILGGVDIPHDRGLLGHSDADALLHAVTDALFGAAALGDIGRHFPDTDPNFAGADSRVLLREAVRRVREAGYEIGNVDATVIAQKPKLAPHISGMVANLAEDLGLPVTRCNVKAKTNEKLGFEGKEEGIVAQAAVLIYRRNGDAQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LMA5</accession>
    <name>Q1LMA5_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Extra-cytoplasmic Solute Receptor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">bug</name>
        <name evidence="4" type="ordered locus">Rmet_1842</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Periplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the UPF0065 (bug) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08721.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516572.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1842"/>
    <dbReference type="EnsemblBacteria" id="ABF08721">
        <property type="protein sequence ID" value="ABF08721"/>
        <property type="gene ID" value="Rmet_1842"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1842"/>
    <dbReference type="eggNOG" id="COG3181">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045683_1_2_4"/>
    <dbReference type="OMA" id="YTTKINW"/>
    <dbReference type="OrthoDB" id="447734at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042597">
        <property type="term" value="C:periplasmic space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005064">
        <property type="entry name" value="BUG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042100">
        <property type="entry name" value="Bug_dom1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006311">
        <property type="entry name" value="TAT_signal"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42928">
        <property type="entry name" value="PTHR42928"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03401">
        <property type="entry name" value="TctC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017082">
        <property type="entry name" value="YflP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51318">
        <property type="entry name" value="TAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0675">Receptor</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193556" evidence="3">
        <location>
            <begin position="29"/>
            <end position="327"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF08721.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="327" mass="35629" checksum="3A0CFAC2319C2AC7" modified="2006-05-30" version="1">MQRRQFIARASLLAATLAAGFAAMPAHAQADKFPQRPIRLVVGYTAGGSTDIPFRVLAENASKILGQPVIVENKPGAGGVLPAQMMQSTQPDGYTLAQVSMPVFRLPYTTKINWDPVKDLTYVINLAGYSFGLVVPADSPIKTMQDYIAYAKANPGKLTYGSPGTMTTLHLTMEDLAMRQGVQFSHIPYKGNSESMQALLGGHVMSVADTPAWAQYVESGKLRLLSTWGEKRSPRFPNVPTLKELGMGIVQASPFGLVVPKGTDPAIVKKLHDAFKKAMEMPNYRESLAKFDMEPNYMSSSQYQAFAADTVKRERAIIEKLGLGKGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LMF6</accession>
    <name>Q1LMF6_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Efflux transporter, RND family, MFP subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Rmet_1791</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08670.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516520.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1791"/>
    <dbReference type="EnsemblBacteria" id="ABF08670">
        <property type="protein sequence ID" value="ABF08670"/>
        <property type="gene ID" value="Rmet_1791"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1791"/>
    <dbReference type="eggNOG" id="COG0845">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018816_1_2_4"/>
    <dbReference type="OMA" id="ATANIIY"/>
    <dbReference type="OrthoDB" id="1432684at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032317">
        <property type="entry name" value="HlyD_D23"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006143">
        <property type="entry name" value="RND_pump_MFP"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16576">
        <property type="entry name" value="HlyD_D23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01730">
        <property type="entry name" value="RND_mfp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="26"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="domain" description="HlyD_D23" evidence="4">
        <location>
            <begin position="78"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="113"/>
            <end position="147"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF16576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABF08670.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="402" mass="42900" checksum="6B7E1E9EB9673D4F" modified="2006-05-30" version="1">MADPDLSKLKIDRSAMAPAQRRRRPWLRYAIIAVVILVLAGIGLKLAGPQTVETATVTSAYPSQNFTLLNATGYVVPQRKAAVASKAQGRLEWLGVLEGSRVKKDEIIARIESRDVEASAAQARAQVQVAEANLALQQAELRDADVNLRRSKELLAPAAISQQQYDSDLARYNKARASISNSEASITSAKANARAAEVAVEQTVVRAPFDGVVLIKHANVGDNITPFSAAADTKGAIVTIADMSTLEVEADVAESNIAKITVEQPCEVLLDALPNLRQEGRVSRIVPTVDRSKATVLVKIRFVDRDPRVLPDMSAKIAFLSKPVAPAENKPLTAIQPSAVVERDGKPVVFLVQDGRAKQAPVTRGAKIGELLAVQGVKPGDVVVLAPSEKLGDGSKVSVAKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="63" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LMZ8</accession>
    <name>Q1LMZ8_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF4136 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_1595</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08478.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516325.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1595"/>
    <dbReference type="EnsemblBacteria" id="ABF08478">
        <property type="protein sequence ID" value="ABF08478"/>
        <property type="gene ID" value="Rmet_1595"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1595"/>
    <dbReference type="eggNOG" id="ENOG5032YB2">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_113282_0_0_4"/>
    <dbReference type="OMA" id="KPGIDKA"/>
    <dbReference type="OrthoDB" id="1557777at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025411">
        <property type="entry name" value="DUF4136"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13590">
        <property type="entry name" value="DUF4136"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF4136" evidence="1">
        <location>
            <begin position="31"/>
            <end position="189"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13590"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF08478.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="194" mass="21608" checksum="C27E4C5117C0DDBA" modified="2006-05-30" version="1">MLSLGGIRRAAPWMVVLVSLAGCATGPDIRADYNHANDFSKYRTFGFVAHPGTDRSGYESLTTQYLKTAVEREMSARGYRYAAHLPDLLVNFNAQLREKVVSNPAPMPPPGYAGYYAYRGGLYAPWPGYGFYSDTYTYTEGTLNIDIVDAQRNQLVWEGVAQGDASDVDPQTRQQAIDKVVTQIFLKYPFRAAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="67" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LN39</accession>
    <name>Q1LN39_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Mating pair formation protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">trbJ</name>
        <name evidence="3" type="ordered locus">Rmet_1554</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08437.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516291.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1554"/>
    <dbReference type="EnsemblBacteria" id="ABF08437">
        <property type="protein sequence ID" value="ABF08437"/>
        <property type="gene ID" value="Rmet_1554"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1554"/>
    <dbReference type="eggNOG" id="COG5314">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_065326_1_0_4"/>
    <dbReference type="OMA" id="SRWENTV"/>
    <dbReference type="OrthoDB" id="1557327at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025415">
        <property type="entry name" value="DUF4141"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014147">
        <property type="entry name" value="T4SS_TrbJ"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13605">
        <property type="entry name" value="DUF4141"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02780">
        <property type="entry name" value="TrbJ_Ti"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193696" evidence="2">
        <location>
            <begin position="22"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="35"/>
            <end position="94"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF08437.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="241" mass="26810" checksum="00DFBED1205AA5A1" modified="2006-05-30" version="1">MKKRLIAAAIAAMLCTATAHAQWVVIDPTNLVQNTLTAIRTLEQINNQIKQLQNEAQMLINQARNLASLPSSVVNQLRTNLATTERLIAQARGLAYDVTNLDREFQRLYPEQYAATVSGDQMYRDAQERWKNTLNGLQTTMQMQAQASQNLHDDEGVLADLVGKSQSAVGALQAMQATNQLLALQAKQSIQTQRLQITQDRAASLELARQAAATERAREVRRRFLGEGTPYTPQSVNFYGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="101" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LNG4</accession>
    <name>Q1LNG4_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Peptide deformylase</fullName>
            <shortName evidence="2">PDF</shortName>
            <ecNumber evidence="2">3.5.1.88</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">Polypeptide deformylase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="3" type="primary">dmf</name>
        <name evidence="2" type="synonym">def</name>
        <name evidence="3" type="ordered locus">Rmet_1429</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>H2O + N-terminal N-formyl-L-methionyl-[peptide] = formate + N-terminal L-methionyl-[peptide]</text>
            <dbReference type="Rhea" id="RHEA:24420"/>
            <dbReference type="Rhea" id="RHEA-COMP:10639"/>
            <dbReference type="Rhea" id="RHEA-COMP:10640"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15740"/>
            <dbReference type="ChEBI" id="CHEBI:49298"/>
            <dbReference type="ChEBI" id="CHEBI:64731"/>
            <dbReference type="EC" id="3.5.1.88"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
        <text evidence="2">Binds 1 Fe(2+) ion.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the polypeptide deformylase family.</text>
    </comment>
    <dbReference type="EC" id="3.5.1.88" evidence="2"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08312.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516180.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1429"/>
    <dbReference type="EnsemblBacteria" id="ABF08312">
        <property type="protein sequence ID" value="ABF08312"/>
        <property type="gene ID" value="Rmet_1429"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1429"/>
    <dbReference type="eggNOG" id="COG0242">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_061901_5_2_4"/>
    <dbReference type="OMA" id="ILYPMRI"/>
    <dbReference type="OrthoDB" id="1649129at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042586">
        <property type="term" value="F:peptide deformylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00487">
        <property type="entry name" value="Pep_deformylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.45.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00163">
        <property type="entry name" value="Pep_deformylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023635">
        <property type="entry name" value="Peptide_deformylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036821">
        <property type="entry name" value="Peptide_deformylase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10458">
        <property type="entry name" value="PTHR10458"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01327">
        <property type="entry name" value="Pep_deformylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004749">
        <property type="entry name" value="Pep_def"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01576">
        <property type="entry name" value="PDEFORMYLASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56420">
        <property type="entry name" value="SSF56420"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00079">
        <property type="entry name" value="pept_deformyl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0408">Iron</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" evidence="2">
        <location>
            <position position="142"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="99"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="141"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="145"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF08312.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="177" mass="20163" checksum="854FDEEB45A85A1B" modified="2006-05-30" version="1">MIRDILKMGDARLLQQARPVTQFNTPELRLLIDDMFDTMEHANGAGLAAPQIGVDLQVVIFGFDRNPRYPDAPTVPKTVLINPSLEPLSDEMDDGWEGCLSVPGLRGVVPRYTRLKYTGFDMMGNRIERVAEDFHARVVQHECDHLIGVLYPMRIKDFTRFGFTEILFPELPPNYDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LNH7</accession>
    <name>Q1LNH7_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">2Fe-2S iron-sulfur cluster binding domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_1416</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08299.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008649088.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1416"/>
    <dbReference type="EnsemblBacteria" id="ABF08299">
        <property type="protein sequence ID" value="ABF08299"/>
        <property type="gene ID" value="Rmet_1416"/>
    </dbReference>
    <dbReference type="GeneID" id="60822172"/>
    <dbReference type="KEGG" id="rme:Rmet_1416"/>
    <dbReference type="eggNOG" id="COG2080">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_052511_2_0_4"/>
    <dbReference type="OMA" id="MMIDFLH"/>
    <dbReference type="OrthoDB" id="1843415at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002888">
        <property type="entry name" value="2Fe-2S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036884">
        <property type="entry name" value="2Fe-2S-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036010">
        <property type="entry name" value="2Fe-2S_ferredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012675">
        <property type="entry name" value="Beta-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01799">
        <property type="entry name" value="Fer2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47741">
        <property type="entry name" value="SSF47741"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54292">
        <property type="entry name" value="SSF54292"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fer2_2" evidence="1">
        <location>
            <begin position="83"/>
            <end position="168"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01799"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF08299.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="184" mass="20037" checksum="502AF5FF2B861289" modified="2006-05-30" version="1">MIATQALSMNINGKDVGPLEVPVGLMMIDFLHEYVNLTGSRLGCGQGVCHACVAILDHPDGTSETIRTCITGAHFFQGKRVRTVEGHGKRNDKGEVVEMTPVQQAFLKHFSFQCGYCTPGFVNGATVLVEKLKREPVAADKVEQTIMSSMDEHICRCTGYVRYYEAIKDVVTSTPGLVKGKGAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LNX3</accession>
    <name>Q1LNX3_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Transport permease protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">ybhR</name>
        <name evidence="7" type="ordered locus">Rmet_1268</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cell inner membrane</location>
            <topology evidence="6">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the ABC-2 integral membrane protein family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08153.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516039.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1268"/>
    <dbReference type="DNASU" id="4038071"/>
    <dbReference type="EnsemblBacteria" id="ABF08153">
        <property type="protein sequence ID" value="ABF08153"/>
        <property type="gene ID" value="Rmet_1268"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1268"/>
    <dbReference type="eggNOG" id="COG0842">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039483_8_3_4"/>
    <dbReference type="OMA" id="WGQELIE"/>
    <dbReference type="OrthoDB" id="1552415at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043190">
        <property type="term" value="C:ATP-binding cassette (ABC) transporter complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000412">
        <property type="entry name" value="ABC_2_transport"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00164">
        <property type="entry name" value="ABC2TRNSPORT"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51012">
        <property type="entry name" value="ABC_TM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4 5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="174"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="228"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="257"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="288"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="349"/>
            <end position="367"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00442"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU361157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABF08153.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="372" mass="40162" checksum="BF8D72B9BC0D925E" modified="2006-05-30" version="1">MLSSAFSFTRLRAQFIKEVLSVLRDPRSRMVVFVPPILQLLVFAFAATLEVRNVDIAVYDQDAGRWSHELVQRLDSARFITHIRHVDSQQQLHELIDRGEVIAALAIPVDFSRSIAAGESGRAQVLVDGRRSNSGQITVAYLSTIAADVGAEVVPDAQAPTPVVVRHWFNPNLVYRWFIVPGLTGILALFSALLITSLSIARERELGTFDQLLVSPTSTPEIIISKSLPALAIGTALGLFMISAGVFLFGIPFTGSFALLLASLVLFILSVVGIGLMISAVSMTQQQAILGAFAIGVPAVLMSGFATPVENMPVVLQWLAQAIPLTHFLIIVEGSFLKAMPPGDILASLWPLAVIALATLTMATVFVRGRLQ</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-10-31" modified="2022-05-25" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LP24</accession>
    <name>LFTR_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Leucyl/phenylalanyl-tRNA--protein transferase</fullName>
            <ecNumber evidence="1">2.3.2.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">L/F-transferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Leucyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Phenyalanyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">aat</name>
        <name type="ordered locus">Rmet_1216</name>
    </gene>
    <organism>
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Functions in the N-end rule pathway of protein degradation where it conjugates Leu, Phe and, less efficiently, Met from aminoacyl-tRNAs to the N-termini of proteins containing an N-terminal arginine or lysine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>L-leucyl-tRNA(Leu) + N-terminal L-lysyl-[protein] = H(+) + N-terminal L-leucyl-L-lysyl-[protein] + tRNA(Leu)</text>
            <dbReference type="Rhea" id="RHEA:12340"/>
            <dbReference type="Rhea" id="RHEA-COMP:9613"/>
            <dbReference type="Rhea" id="RHEA-COMP:9622"/>
            <dbReference type="Rhea" id="RHEA-COMP:12670"/>
            <dbReference type="Rhea" id="RHEA-COMP:12671"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:65249"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78494"/>
            <dbReference type="ChEBI" id="CHEBI:133043"/>
            <dbReference type="EC" id="2.3.2.6"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>L-leucyl-tRNA(Leu) + N-terminal L-arginyl-[protein] = H(+) + N-terminal L-leucyl-L-arginyl-[protein] + tRNA(Leu)</text>
            <dbReference type="Rhea" id="RHEA:50416"/>
            <dbReference type="Rhea" id="RHEA-COMP:9613"/>
            <dbReference type="Rhea" id="RHEA-COMP:9622"/>
            <dbReference type="Rhea" id="RHEA-COMP:12672"/>
            <dbReference type="Rhea" id="RHEA-COMP:12673"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:64719"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78494"/>
            <dbReference type="ChEBI" id="CHEBI:133044"/>
            <dbReference type="EC" id="2.3.2.6"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>an N-terminal L-alpha-aminoacyl-[protein] + L-phenylalanyl-tRNA(Phe) = an N-terminal L-phenylalanyl-L-alpha-aminoacyl-[protein] + tRNA(Phe)</text>
            <dbReference type="Rhea" id="RHEA:43632"/>
            <dbReference type="Rhea" id="RHEA-COMP:9668"/>
            <dbReference type="Rhea" id="RHEA-COMP:9699"/>
            <dbReference type="Rhea" id="RHEA-COMP:10636"/>
            <dbReference type="Rhea" id="RHEA-COMP:10637"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78531"/>
            <dbReference type="ChEBI" id="CHEBI:78597"/>
            <dbReference type="ChEBI" id="CHEBI:83561"/>
            <dbReference type="EC" id="2.3.2.6"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the L/F-transferase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.6" evidence="1"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08102.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515992.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q1LP24"/>
    <dbReference type="SMR" id="Q1LP24"/>
    <dbReference type="STRING" id="266264.Rmet_1216"/>
    <dbReference type="EnsemblBacteria" id="ABF08102">
        <property type="protein sequence ID" value="ABF08102"/>
        <property type="gene ID" value="Rmet_1216"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1216"/>
    <dbReference type="eggNOG" id="COG2360">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_075045_0_0_4"/>
    <dbReference type="OMA" id="YRQGIFP"/>
    <dbReference type="OrthoDB" id="1670846at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008914">
        <property type="term" value="F:leucyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030163">
        <property type="term" value="P:protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.3550">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00688">
        <property type="entry name" value="Leu_Phe_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004616">
        <property type="entry name" value="Leu/Phe-tRNA_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042203">
        <property type="entry name" value="Leu/Phe-tRNA_Trfase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042221">
        <property type="entry name" value="Leu/Phe-tRNA_Trfase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30098">
        <property type="entry name" value="PTHR30098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03588">
        <property type="entry name" value="Leu_Phe_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="SSF55729"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00667">
        <property type="entry name" value="aat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0012">Acyltransferase</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000258084" description="Leucyl/phenylalanyl-tRNA--protein transferase">
        <location>
            <begin position="1"/>
            <end position="249"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00688"/>
        </source>
    </evidence>
    <sequence length="249" mass="28028" checksum="E04684097E1ACF37" modified="2006-05-30" version="1">MITWLDPQDPFPPVERALGPASDAPGLLAASRDLSPQRLLLAYRQGIFPWYSEGQPVLWWSTDPRMVLAPHRLKISVSLRKTLRRILRDPDWEIRVDDDFVAVMQACAMTPRDGQLGTWITDDIVAAYGSLHRLGLAHSVETWYRGERVGGLYGVALGRMFYGESMFAHRTDASKIALAALCGFLERHGVTMIDCQQETDHLASLGAEPIPREQFIAHVRQTAAEANISPWRFDKSELTRWTSQASTEL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2007-05-29" modified="2022-05-25" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LPE1</accession>
    <name>GCH41_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">GTP cyclohydrolase FolE2 1</fullName>
            <ecNumber evidence="1">3.5.4.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">folE2-1</name>
        <name type="ordered locus">Rmet_1099</name>
    </gene>
    <organism>
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Converts GTP to 7,8-dihydroneopterin triphosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>GTP + H2O = 7,8-dihydroneopterin 3'-triphosphate + formate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17473"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15740"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:58462"/>
            <dbReference type="EC" id="3.5.4.16"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; 7,8-dihydroneopterin triphosphate biosynthesis; 7,8-dihydroneopterin triphosphate from GTP: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the GTP cyclohydrolase IV family.</text>
    </comment>
    <comment type="sequence caution" evidence="2">
        <conflict type="erroneous initiation">
            <sequence resource="EMBL-CDS" id="ABF07985" version="1"/>
        </conflict>
    </comment>
    <dbReference type="EC" id="3.5.4.16" evidence="1"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07985.1"/>
        <property type="status" value="ALT_INIT"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_029309978.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q1LPE1"/>
    <dbReference type="SMR" id="Q1LPE1"/>
    <dbReference type="STRING" id="266264.Rmet_1099"/>
    <dbReference type="EnsemblBacteria" id="ABF07985">
        <property type="protein sequence ID" value="ABF07985"/>
        <property type="gene ID" value="Rmet_1099"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1099"/>
    <dbReference type="eggNOG" id="COG1469">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_062816_0_0_4"/>
    <dbReference type="OrthoDB" id="757842at2"/>
    <dbReference type="UniPathway" id="UPA00848">
        <property type="reaction ID" value="UER00151"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003934">
        <property type="term" value="F:GTP cyclohydrolase I activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035998">
        <property type="term" value="P:7,8-dihydroneopterin 3'-triphosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01527_B">
        <property type="entry name" value="GTP_cyclohydrol_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022838">
        <property type="entry name" value="GTP_cyclohydrolase_FolE2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003801">
        <property type="entry name" value="GTP_cyclohydrolase_FolE2/MptA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36445">
        <property type="entry name" value="PTHR36445"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02649">
        <property type="entry name" value="GCHY-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000289517" description="GTP cyclohydrolase FolE2 1">
        <location>
            <begin position="1"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="site" description="May be catalytically important" evidence="1">
        <location>
            <position position="156"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="309" mass="33970" checksum="FFF204BF8A9293C6" modified="2009-05-05" version="2">MSAALPDVSLTEASPTFTPLEWVGMQGIDLPIRLVEPGYRRQLHARADIHVDLPAPRVKGIHMSRLYALLDALDDGEGLSPIRIRQLLEAMVDSHRDCETGSARLRLRFDLLVRRPALVTEGIAGWKSYPVRLDASLVGNVFALTARITAGYSSTCPCSAALTRQLVEQGFAKAFAHERRVDVARVSAWLRQHGTLATPHSQRSEASITVDIAVDLPNLGLLDLVDHIERALGTPVQTAVKRADEQAFAALNGQNLMFVEDAARRIQVALDGRFANPRVHVRHLESLHPHDAVAWAAPLQMKHSREVAA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-03-03" modified="2022-08-03" version="99" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LPH7</accession>
    <name>FENR1_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Ferredoxin--NADP reductase 1</fullName>
            <shortName evidence="1">FNR 1</shortName>
            <shortName evidence="1">Fd-NADP(+) reductase 1</shortName>
            <ecNumber evidence="1">1.18.1.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">Rmet_1063</name>
    </gene>
    <organism>
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>H(+) + NADP(+) + 2 reduced [2Fe-2S]-[ferredoxin] = NADPH + 2 oxidized [2Fe-2S]-[ferredoxin]</text>
            <dbReference type="Rhea" id="RHEA:20125"/>
            <dbReference type="Rhea" id="RHEA-COMP:10000"/>
            <dbReference type="Rhea" id="RHEA-COMP:10001"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33737"/>
            <dbReference type="ChEBI" id="CHEBI:33738"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.18.1.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
        <text evidence="1">Binds 1 FAD per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the ferredoxin--NADP reductase type 2 family.</text>
    </comment>
    <dbReference type="EC" id="1.18.1.2" evidence="1"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07949.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515851.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q1LPH7"/>
    <dbReference type="SMR" id="Q1LPH7"/>
    <dbReference type="STRING" id="266264.Rmet_1063"/>
    <dbReference type="EnsemblBacteria" id="ABF07949">
        <property type="protein sequence ID" value="ABF07949"/>
        <property type="gene ID" value="Rmet_1063"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1063"/>
    <dbReference type="eggNOG" id="COG0492">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031864_5_5_4"/>
    <dbReference type="OMA" id="LEQCAPF"/>
    <dbReference type="OrthoDB" id="692968at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004324">
        <property type="term" value="F:ferredoxin-NADP+ reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01685">
        <property type="entry name" value="FENR2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023753">
        <property type="entry name" value="FAD/NAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022890">
        <property type="entry name" value="Fd--NADP_Rdtase_type_2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07992">
        <property type="entry name" value="Pyr_redox_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="SSF51905"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0274">FAD</keyword>
    <keyword id="KW-0285">Flavoprotein</keyword>
    <keyword id="KW-0521">NADP</keyword>
    <keyword id="KW-0560">Oxidoreductase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000364907" description="Ferredoxin--NADP reductase 1">
        <location>
            <begin position="1"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="43"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="51"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="56"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="96"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="135"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="300"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="341"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01685"/>
        </source>
    </evidence>
    <sequence length="353" mass="37657" checksum="D6DBE36B23B96783" modified="2006-05-30" version="1">MSTTPTSAPETTQTDVLIVGAGPVGLFAAFQAGVLGLKCEIVDVLDRAGGQCTELYPEKPIYDIPAVPGCLAQDLVDRLLEQCAPFAFPMHFSQRAESVSDTTCTNGHPRLLVTTDAGKRFDVAAVLICAGAGAFAPQRVSLPEAAALEDRHLHYAVRDLSRFAGKRVIVAGGGDSALDWAMALRKTAARVTLLHRREGFRAADHTVKAMRDAVAAGEMDFVVGMLGGLQTNGDGALTGAVVKSRDGEQTIPADDLVALYGLVSEPGPIAQWDMDMRAGRIAVDTTTYESSRRGIFAAGDIAFYPNKQKLILSGFHEAALALRKAYHYAFPEKSLVHVHTSNNAALKERLTHG</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LPM8</accession>
    <name>Q1LPM8_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Flavin-binding monooxygenase</fullName>
            <ecNumber evidence="5">1.14.13.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Rmet_1012</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the FAD-binding monooxygenase family.</text>
    </comment>
    <dbReference type="EC" id="1.14.13.-" evidence="5"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07898.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515811.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1012"/>
    <dbReference type="EnsemblBacteria" id="ABF07898">
        <property type="protein sequence ID" value="ABF07898"/>
        <property type="gene ID" value="Rmet_1012"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1012"/>
    <dbReference type="eggNOG" id="COG2072">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032067_2_0_4"/>
    <dbReference type="OMA" id="ASWTLKC"/>
    <dbReference type="OrthoDB" id="324612at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004499">
        <property type="term" value="F:N,N-dimethylaniline monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020946">
        <property type="entry name" value="Flavin_mOase-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00743">
        <property type="entry name" value="FMO-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="SSF51905"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0274">FAD</keyword>
    <keyword evidence="2" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="5" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="4 5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABF07898.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="505" mass="56394" checksum="1E73F7532D398138" modified="2006-05-30" version="1">MTAPTSDDAVLDVLIVGAGLSGIGAARQLQDRCPGKRYAILEARQAMGGTWDLFRYPGIRSDSDMYTLGYRFKPWRGAKAIADGPSILSYIRETADEAGITKHIRYGHKVVSAAWDSRSACWTIEAERTADGSRVRLRAHFLYVCAGYYSYAEGHRPTFPGEENFRGRMVHPQFWDPSIDYAGKRVVVIGSGATAVTLVPSMSQTAAHVTMLQRSPTYIVTRPGQDAIAHKLRRMLPEGLAYAATRWKNVLLGMFFFQLARRKPEKVKARMIGMAAEQLAPGYDVDTHFTPRYKPWDQRVCLVPDGDLFREIREGRASIVTDTIERFTEDGIVLASGQTLPADIVVMATGLKLNMLGDIVVTVDGVPRRPAEAMAYKGMMLSDVPNLVLAFGYTNASWTLKADLTAEYVCRLLRYMDRHGYRSAVPHVDPTVQTMPFLDFSSGYVQRAASVLPRQGDRRPWRVYQNYFMDMMTIRHGRIADGVLQFDRQGAATLPQEDALAEVQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="53" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LPS4</accession>
    <name>Q1LPS4_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_0966</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07852.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515771.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF07852">
        <property type="protein sequence ID" value="ABF07852"/>
        <property type="gene ID" value="Rmet_0966"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0966"/>
    <dbReference type="HOGENOM" id="CLU_992951_0_0_4"/>
    <dbReference type="OMA" id="HEWWGCL"/>
    <dbReference type="OrthoDB" id="1993825at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF07852.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="229" mass="24228" checksum="2024BD4A07F1A28B" modified="2006-05-30" version="1">MRIYTFFHAHHRGDLPHLKTATTNGFGHCLCVIPTTAGLGHSCDYIHLRAGDCYPVTVAGHPEWWACLQGGLRVLDGDAERAVLACGDIHTPEVHQAVELQAVVDSVLVRAIPQAGEAGHCNAGAAEFIAIDAGEAEWAQHLHARGLVWGVCHRGVLNLQWRGGASGGEVQSTYLQPGMAFAPEPDDDYCIDAMLSGAGLLCCMQPAPAPAPVPVPSDAYAPQGERQAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="51" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LQ11</accession>
    <name>Q1LQ11_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_0879</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07765.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515706.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF07765">
        <property type="protein sequence ID" value="ABF07765"/>
        <property type="gene ID" value="Rmet_0879"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0879"/>
    <dbReference type="HOGENOM" id="CLU_2571368_0_0_4"/>
    <dbReference type="OMA" id="NNPECGE"/>
    <dbReference type="OrthoDB" id="1845640at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF07765.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="81" mass="9398" checksum="60CE3E2A5DDD677A" modified="2006-05-30" version="1">MRRYVKSRSELMAILSQCLDNNPECGEVELHAMRVHQPDHTGCNWSAEVDFRQETFTDLAQQMAAARSIIVVMREQYNVLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="103" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LQ12</accession>
    <name>Q1LQ12_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="13">NADP-dependent fused malic enzyme oxidoreductase phosphotransacetylase</fullName>
            <ecNumber evidence="13">1.1.1.40</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">maeB</name>
        <name evidence="13" type="ordered locus">Rmet_0878</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="4">In the C-terminal section; belongs to the phosphate acetyltransferase and butyryltransferase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">In the N-terminal section; belongs to the malic enzymes family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.40" evidence="13"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07764.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515705.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0878"/>
    <dbReference type="EnsemblBacteria" id="ABF07764">
        <property type="protein sequence ID" value="ABF07764"/>
        <property type="gene ID" value="Rmet_0878"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0878"/>
    <dbReference type="eggNOG" id="COG0280">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG0281">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012366_0_0_4"/>
    <dbReference type="OMA" id="KPVHILN"/>
    <dbReference type="OrthoDB" id="654531at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016746">
        <property type="term" value="F:acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004471">
        <property type="term" value="F:malate dehydrogenase (decarboxylating) (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004473">
        <property type="term" value="F:malate dehydrogenase (decarboxylating) (NADP+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008948">
        <property type="term" value="F:oxaloacetate decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006108">
        <property type="term" value="P:malate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05311">
        <property type="entry name" value="NAD_bind_2_malic_enz"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10380">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10750">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10950">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046346">
        <property type="entry name" value="Aminiacid_DH-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015884">
        <property type="entry name" value="Malic_enzyme_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012301">
        <property type="entry name" value="Malic_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037062">
        <property type="entry name" value="Malic_N_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012302">
        <property type="entry name" value="Malic_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045213">
        <property type="entry name" value="Malic_NAD-bd_bact_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012188">
        <property type="entry name" value="ME_PTA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042113">
        <property type="entry name" value="P_AcTrfase_dom1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042112">
        <property type="entry name" value="P_AcTrfase_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002505">
        <property type="entry name" value="PTA_PTB"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00390">
        <property type="entry name" value="malic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03949">
        <property type="entry name" value="Malic_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01515">
        <property type="entry name" value="PTA_PTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036684">
        <property type="entry name" value="ME_PTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01274">
        <property type="entry name" value="malic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00919">
        <property type="entry name" value="Malic_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53223">
        <property type="entry name" value="SSF53223"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00331">
        <property type="entry name" value="MALIC_ENZYMES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="10" id="KW-0521">NADP</keyword>
    <keyword evidence="6 13" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="malic" evidence="12">
        <location>
            <begin position="34"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="domain" description="Malic_M" evidence="11">
        <location>
            <begin position="179"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="8">
        <location>
            <position position="110"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="92"/>
            <end position="99"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="152"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="153"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="178"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="303"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007686"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008756"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR036684-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR036684-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR036684-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM01274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ABF07764.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="773" mass="83025" checksum="CFA1A6C67B96E071" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LQ67</accession>
    <name>Q1LQ67_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Extra-cytoplasmic Solute Receptor protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">bug</name>
        <name evidence="4" type="ordered locus">Rmet_0823</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Periplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the UPF0065 (bug) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07709.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515653.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0823"/>
    <dbReference type="EnsemblBacteria" id="ABF07709">
        <property type="protein sequence ID" value="ABF07709"/>
        <property type="gene ID" value="Rmet_0823"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0823"/>
    <dbReference type="eggNOG" id="COG3181">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045683_0_0_4"/>
    <dbReference type="OMA" id="YNTETDF"/>
    <dbReference type="OrthoDB" id="447734at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042597">
        <property type="term" value="C:periplasmic space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005064">
        <property type="entry name" value="BUG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042100">
        <property type="entry name" value="Bug_dom1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42928">
        <property type="entry name" value="PTHR42928"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03401">
        <property type="entry name" value="TctC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017082">
        <property type="entry name" value="YflP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0675">Receptor</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193426" evidence="3">
        <location>
            <begin position="26"/>
            <end position="325"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF07709.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="325" mass="34414" checksum="4726F79D14497CD2" modified="2006-05-30" version="1">MKTWLRLFGVGLATSLTLAAAPSHAENYPSKPIRLIVPFPASGATDLLARAIAQKVGTSMGQQIVVDNRPGAGGAIGSDMAAKAPADGYTLLIATTSTHSIGPYINTHLPYNTETDFTPIGQVATATNVLVVPNSLPVKNVKELIDYAKKHPGELNYASSGNGTVVHLTAEAFKAQAGVFITHIPYRGTALAVPDLISNKVQILFDSIVSGMPHVKNGKLKGLAVTSMKRSPLAPDLPTVNESGLPGFESDTWFGIYGPKGLPADIVNRLNSEFNKAIQSQDVKERLAKLGAEPVGGTPAQFAAMVKKDSARWGKLIKDRKIQVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-12-02" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LQB3</accession>
    <name>Q1LQB3_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Bcr/CflA family efflux transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">bcr</name>
        <name evidence="9" type="ordered locus">Rmet_0777</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cell inner membrane</location>
            <topology evidence="7">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the major facilitator superfamily. Bcr/CmlA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07663.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0777"/>
    <dbReference type="EnsemblBacteria" id="ABF07663">
        <property type="protein sequence ID" value="ABF07663"/>
        <property type="gene ID" value="Rmet_0777"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0777"/>
    <dbReference type="eggNOG" id="COG2814">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001265_47_1_4"/>
    <dbReference type="OMA" id="FNIICVV"/>
    <dbReference type="OrthoDB" id="1452500at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042910">
        <property type="term" value="F:xenobiotic transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990961">
        <property type="term" value="P:xenobiotic detoxification by transmembrane export across the plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004812">
        <property type="entry name" value="Efflux_drug-R_Bcr/CmlA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="SSF103473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00710">
        <property type="entry name" value="efflux_Bcr_CflA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="7" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 7" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="28"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="63"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="93"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="118"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="151"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="181"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="231"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="269"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="389"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="domain" description="MFS" evidence="8">
        <location>
            <begin position="24"/>
            <end position="412"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU365088"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ABF07663.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="418" mass="43882" checksum="895D751FEC48A678" modified="2006-05-30" version="1">MSEVDVPGAVVAAGAEAGPRRPSVGSMAALTAGLSMLGQFAIATYLPAFAAIANALHASTAEVQQSLTAYLLPFALLLPWHGAISDAIGRRRMILAGCVFFVAGSLVCAGATGIDMFYVGRALQGVGAGIGVIVGRAMVRDTFDGMQAQKVMALIAMIFALAPALAPICGGWLLVWMGWRSIFVFLALISGSLLVSCWIWVPETLPPACRHPMHPKVLARGYFAMLSSRRFMSLALANAGVNLAIYIYVFAAPRFVYQHLGLGAQSFGWLFIPIVAGIIAGALVAHRIAGRTTPESSVNRGHVVMMAAGVFNLAVAALHLPPMPWALVALPVFAAGMMMTQPSLQLLALDCFPERRGLASSGYVTAQQIGNLLSSALLVPLLLDSTMKMALGMAGLQIAGLVAFHVALRGRRVGVTSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="99" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LQC8</accession>
    <name>Q1LQC8_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Carbon-phosphorus lyase complex subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">phnK</name>
        <name evidence="9" type="ordered locus">Rmet_0762</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07648.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515603.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0762"/>
    <dbReference type="EnsemblBacteria" id="ABF07648">
        <property type="protein sequence ID" value="ABF07648"/>
        <property type="gene ID" value="Rmet_0762"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0762"/>
    <dbReference type="eggNOG" id="COG4107">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_23_4"/>
    <dbReference type="OMA" id="TDWGFVR"/>
    <dbReference type="OrthoDB" id="1101128at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012700">
        <property type="entry name" value="CP_lyase_PhnK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037116">
        <property type="entry name" value="CP_lyase_PhnK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02323">
        <property type="entry name" value="CP_lyasePhnK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="1" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-0456">Lyase</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-1278">Translocase</keyword>
    <feature type="domain" description="ABC transporter" evidence="8">
        <location>
            <begin position="7"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="39"/>
            <end position="46"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ABF07648.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="258" mass="28388" checksum="4A41242F1A775810" modified="2006-05-30" version="1">MNATPLLSVRNLTHTWDGVHGCHDVNFDLYPGEVLCVVGESGSGKSTLLQALSMQVKAQRGSVSYDMRESGLTDLAGLSSARMRLLARTDWGFVRQHARDGLRMQVSAGANIAERLMAVGNRHYGDLREIAGAWLEKMEIDLSRLDDVPTTFSGGMQQRLQIARNLVTHPRLVFMDEPTASLDVSVQARLLDLMRRLVTDLGLAAILVTHDLAVARLLAHRTLVMQGGRVVEQGLTDQILDDPQHPYTQLLVSSILQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-10-07" version="59" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LQI4</accession>
    <name>Q1LQI4_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF1854 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_0706</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07592.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0706"/>
    <dbReference type="EnsemblBacteria" id="ABF07592">
        <property type="protein sequence ID" value="ABF07592"/>
        <property type="gene ID" value="Rmet_0706"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0706"/>
    <dbReference type="eggNOG" id="ENOG5031GY9">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_121383_1_0_4"/>
    <dbReference type="OMA" id="FSTPSTW"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015005">
        <property type="entry name" value="DUF1854"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08909">
        <property type="entry name" value="DUF1854"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF1854" evidence="2">
        <location>
            <begin position="41"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF08909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF07592.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="171" mass="18944" checksum="05976D86326E6649" modified="2006-05-30" version="1">MRSNQTSEQTQEQPAMQTPDFNLSRNSFGKLVLTLADGLSHDGVVPVRAFPISAPDGGVGMMSTDGRELAWIPSLDGLPAPIRELIEAELAAREFMPEIRKIVGVSTYATPSVWTVQTDRGQTDLVLRGEEDIRRLVGNTLLISDSHGIHYLIRDLMALDKPSRKIMDRFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LQK7</accession>
    <name>Q1LQK7_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Smr protein/MutS2 C-terminal</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_0683</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07569.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515536.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0683"/>
    <dbReference type="EnsemblBacteria" id="ABF07569">
        <property type="protein sequence ID" value="ABF07569"/>
        <property type="gene ID" value="Rmet_0683"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0683"/>
    <dbReference type="eggNOG" id="COG2840">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_055978_1_0_4"/>
    <dbReference type="OMA" id="MIKSHVN"/>
    <dbReference type="OrthoDB" id="1888882at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002625">
        <property type="entry name" value="Smr_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036063">
        <property type="entry name" value="Smr_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01713">
        <property type="entry name" value="Smr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00463">
        <property type="entry name" value="SMR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160443">
        <property type="entry name" value="SSF160443"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50828">
        <property type="entry name" value="SMR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Smr" evidence="2">
        <location>
            <begin position="139"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="17"/>
            <end position="45"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50828"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF07569.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="224" mass="25038" checksum="0E04C280A8E6D732" modified="2006-05-30" version="1">MSHGAKPTSKPPHRMGLHDLATVRDGLKADAERREAERQAAEAAARRAEEEANVFRTSIGQVSNLRDRNRVEHPVKKPNPEPVQTRLNDKAVLEASLSDEFDVENLLDVDETMSFRRPGIGEDVIKKLRRGEWVPQDKVDLHGLRSDEAREALANFLRRSVRNGVRCVRVIHGKGIGSPDKLPVLKGKVRSWLVQKEEVIAFVQARESQGGAGALMVLLRQPKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LQP1</accession>
    <name>Q1LQP1_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Predicted ATPase with chaperone activity</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_0649</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07535.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0649"/>
    <dbReference type="EnsemblBacteria" id="ABF07535">
        <property type="protein sequence ID" value="ABF07535"/>
        <property type="gene ID" value="Rmet_0649"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0649"/>
    <dbReference type="eggNOG" id="COG1239">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029703_1_0_4"/>
    <dbReference type="OMA" id="EKFEVPF"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000523">
        <property type="entry name" value="Mg_chelatse_chII-like_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01078">
        <property type="entry name" value="Mg_chelatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA" evidence="2">
        <location>
            <begin position="180"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="449"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="454"/>
            <end position="470"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF07535.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="470" mass="51527" checksum="FE57DD2BD199F8C7" modified="2006-05-30" version="1">MLEHHEAGQATRLPEAVASMVRHAQADHDALPAWHVPPRSIADTGLEIALLLGLLLKAAYLHRSVSLTILTDTLKLPASVVNEIATVAVRERLLEVAHRGASDIDVRFRLTDAGYARAAETSARCSYSGAAPVTLQAYLDAIQHHSIHHTSVTKADVTAAFHNLVAPVHLLDSIGMALNTARALMIYGPPGSGKTYLAQRLGALLPGAVPVPHAITVAGEIIQIMDPLVHTPIDDSEASSARRSIDRRWVVCHRPVVLTGGELTLSMLDLRYDSNSGFYQAPPHMKANNGIYIVDDLGRQMVGVEQLLNRWIVPLDRGIDMFTLHSGVRFSVPFDVWPVFSTNLSPTDLSDDAFLRRLGSKLYVGPLPIDDYREVCLRTATELGLTWPEPAFDFLLESLHFSSDTPLLACIPRDLLRLVASHVRYHGHAPQVTEESLCRAWQSYYGLQGKPEAPPPDNEHRWPAGHRAAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LQP3</accession>
    <name>Q1LQP3_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Flp pilus assembly protein secretin CpaC</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">cpaC1</name>
        <name evidence="5" type="ordered locus">Rmet_0647</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the bacterial secretin family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07533.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515504.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0647"/>
    <dbReference type="EnsemblBacteria" id="ABF07533">
        <property type="protein sequence ID" value="ABF07533"/>
        <property type="gene ID" value="Rmet_0647"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0647"/>
    <dbReference type="eggNOG" id="COG4964">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017952_1_0_4"/>
    <dbReference type="OMA" id="TELMIMV"/>
    <dbReference type="OrthoDB" id="870677at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009306">
        <property type="term" value="P:protein secretion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001775">
        <property type="entry name" value="GspD/PilQ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032789">
        <property type="entry name" value="T2SS-T3SS_pil_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004846">
        <property type="entry name" value="T2SS/T3SS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00263">
        <property type="entry name" value="Secretin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13629">
        <property type="entry name" value="T2SS-T3SS_pil_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00811">
        <property type="entry name" value="BCTERIALGSPD"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193713" evidence="3">
        <location>
            <begin position="29"/>
            <end position="620"/>
        </location>
    </feature>
    <feature type="domain" description="T2SS-T3SS_pil_N" evidence="4">
        <location>
            <begin position="90"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="488"/>
            <end position="589"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="505"/>
            <end position="529"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="530"/>
            <end position="544"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="557"/>
            <end position="571"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU004003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF13629"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABF07533.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="620" mass="62939" checksum="24DDA5220FAAF08B" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-10-07" version="50" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LQQ8</accession>
    <name>Q1LQQ8_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_0632</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07518.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0632"/>
    <dbReference type="EnsemblBacteria" id="ABF07518">
        <property type="protein sequence ID" value="ABF07518"/>
        <property type="gene ID" value="Rmet_0632"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0632"/>
    <dbReference type="eggNOG" id="ENOG502Z9U6">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_053135_0_0_4"/>
    <dbReference type="OMA" id="PQTAWNG"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="34"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="68"/>
            <end position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF07518.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="425" mass="46165" checksum="99F1F783387A4D9A" modified="2006-05-30" version="1">MWLQADIEPYLSATMPIELPEPPSGEGAPKPPNLWVWLVLLVVCLAAGIVWTLLTWPKGQSTGTSRFWMQLLGYPTLACAVAFGLRLHFYEEACNYLMAQEEVLQADREEAIAFGSEPLVVIGIAYLCGMGSSGVSGRVAQKESALEARAPKRDMAPIRHTRLALPEEYQGTDRYRQVFRALLAKLDHALCALPSGVPFDVQLQLPGDADSEPLLTIWSTCWDDCGLRPTKARLLPDGAGLMALDTWLDEYGGPALEKFTLFVALQLNDEPPANSAEVAVALLLGWPPMAQRRDVLPIAMLHRPVACETDGFAESLRTVALLGRTPPNELHQLWQSSLSKVDKAALLKSASEVSLEAAEADDLSGIHDIDAALGNPGVAASWFATALAVEYAQQNNASQLIACQEDTLRLAVVRPTDSQAELETT</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="64" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LQR9</accession>
    <name>Q1LQR9_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DotU domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_0621</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07507.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515478.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0621"/>
    <dbReference type="EnsemblBacteria" id="ABF07507">
        <property type="protein sequence ID" value="ABF07507"/>
        <property type="gene ID" value="Rmet_0621"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0621"/>
    <dbReference type="eggNOG" id="COG3455">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_071818_2_1_4"/>
    <dbReference type="OMA" id="HAQCALL"/>
    <dbReference type="OrthoDB" id="1650600at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.590">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017732">
        <property type="entry name" value="T4/T6SS_DotU"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038522">
        <property type="entry name" value="T4/T6SS_DotU_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09850">
        <property type="entry name" value="DotU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03349">
        <property type="entry name" value="IV_VI_DotU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="188"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="domain" description="DotU" evidence="2">
        <location>
            <begin position="11"/>
            <end position="206"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF09850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF07507.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="218" mass="23778" checksum="C7900F11A2D37169" modified="2006-05-30" version="1">MTTLTTILPLALRDTALTVTELADEAAPDDSFAMFRKKCLEQVTRLREEFLAAGHAPAVVEDAAYAQCALLDEYALRRLTGDERSAWEREPLQVQEFHSHNAGEELIARIERRLAEAQPVIPLLVVFHAVLGLGFQGKFALEGNAAREALMHAIDERLQRAGVQKATGPVIVTAGKARGWRGLSLSPLAWVAIALVGAGLVYFALDRWLAVSIARLAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="100" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LR53</accession>
    <name>Q1LR53_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Zinc metalloprotease, peptidase family M48</fullName>
            <ecNumber evidence="9">3.4.24.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">Rmet_0487</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <dbReference type="EC" id="3.4.24.-" evidence="9"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07373.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0487"/>
    <dbReference type="EnsemblBacteria" id="ABF07373">
        <property type="protein sequence ID" value="ABF07373"/>
        <property type="gene ID" value="Rmet_0487"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0487"/>
    <dbReference type="eggNOG" id="COG4783">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030556_1_0_4"/>
    <dbReference type="OMA" id="HLSQRHF"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001915">
        <property type="entry name" value="Peptidase_M48"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01435">
        <property type="entry name" value="Peptidase_M48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="SSF48452"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 9" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="2 9" id="KW-0645">Protease</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Peptidase_M48" evidence="8">
        <location>
            <begin position="194"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="64"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="118"/>
            <end position="138"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ABF07373.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="605" mass="65281" checksum="78388BB3705C737F" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="113" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LR70</accession>
    <name>Q1LR70_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Succinate--CoA ligase [ADP-forming] subunit alpha</fullName>
            <ecNumber evidence="2">6.2.1.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">Succinyl-CoA synthetase subunit alpha</fullName>
            <shortName evidence="2">SCS-alpha</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="2 7" type="primary">sucD</name>
        <name evidence="7" type="ordered locus">Rmet_0470</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2 5">Succinyl-CoA synthetase functions in the citric acid cycle (TCA), coupling the hydrolysis of succinyl-CoA to the synthesis of either ATP or GTP and thus represents the only step of substrate-level phosphorylation in the TCA. The alpha subunit of the enzyme binds the substrates coenzyme A and phosphate, while succinate binding and nucleotide specificity is provided by the beta subunit.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2 5">
            <text>ATP + CoA + succinate = ADP + phosphate + succinyl-CoA</text>
            <dbReference type="Rhea" id="RHEA:17661"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57292"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.2.1.5"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 5">Carbohydrate metabolism; tricarboxylic acid cycle; succinate from succinyl-CoA (ligase route): step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2 5">Heterotetramer of two alpha and two beta subunits.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 4">Belongs to the succinate/malate CoA ligase alpha subunit family.</text>
    </comment>
    <dbReference type="EC" id="6.2.1.5" evidence="2"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07356.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008646503.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0470"/>
    <dbReference type="EnsemblBacteria" id="ABF07356">
        <property type="protein sequence ID" value="ABF07356"/>
        <property type="gene ID" value="Rmet_0470"/>
    </dbReference>
    <dbReference type="GeneID" id="60824075"/>
    <dbReference type="KEGG" id="rme:Rmet_0470"/>
    <dbReference type="eggNOG" id="COG0074">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_052104_0_0_4"/>
    <dbReference type="OMA" id="TVIFIPP"/>
    <dbReference type="OrthoDB" id="1081709at2"/>
    <dbReference type="UniPathway" id="UPA00223">
        <property type="reaction ID" value="UER00999"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004775">
        <property type="term" value="F:succinate-CoA ligase (ADP-forming) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.261">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01988">
        <property type="entry name" value="Succ_CoA_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017440">
        <property type="entry name" value="Cit_synth/succinyl-CoA_lig_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033847">
        <property type="entry name" value="Citrt_syn/SCS-alpha_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003781">
        <property type="entry name" value="CoA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005810">
        <property type="entry name" value="CoA_lig_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005811">
        <property type="entry name" value="CoA_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016102">
        <property type="entry name" value="Succinyl-CoA_synth-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02629">
        <property type="entry name" value="CoA_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00549">
        <property type="entry name" value="Ligase_CoA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001553">
        <property type="entry name" value="SucCS_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00881">
        <property type="entry name" value="CoA_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52210">
        <property type="entry name" value="SSF52210"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01019">
        <property type="entry name" value="sucCoAalpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01216">
        <property type="entry name" value="SUCCINYL_COA_LIG_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00399">
        <property type="entry name" value="SUCCINYL_COA_LIG_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4 7" id="KW-0436">Ligase</keyword>
    <keyword evidence="1 2 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0816">Tricarboxylic acid cycle</keyword>
    <feature type="domain" description="CoA_binding" evidence="6">
        <location>
            <begin position="4"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="region of interest" description="Coenzyme A binding" evidence="2">
        <location>
            <begin position="17"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Coenzyme A binding" evidence="2">
        <location>
            <begin position="99"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="active site" description="Tele-phosphohistidine intermediate" evidence="2 3">
        <location>
            <position position="252"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="46"/>
        </location>
        <ligand>
            <name>CoA</name>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="164"/>
        </location>
        <ligand>
            <name>substrate</name>
            <note>ligand shared with subunit beta</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01988"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR001553-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU000699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABF07356.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="293" mass="30907" checksum="C3EB0AF0877CFB4C" modified="2006-05-30" version="1">MSILINKDTKVITQGITGKTGQFHTRGCRDYANGKNCFVAGVNPKKAGEDFEGIPIYASVKDAKEQTGATVSVIYVPPAGAAAAIWEAVDADLDLVVCITEGIPVRDMMEVKDKMRRQNKKTLLLGPNCPGLITPDEIKIGIMPGHIHRKGRIGVVSRSGTLTYEAVGQLTALGLGQSSAVGIGGDPINGLKHIDVMKMFNDDPETDAVVMIGEIGGPDEANAAYWIKENMKKPVVGFIAGVTAPPGKRMGHAGALISGGADTAQAKLEIMEACGIKVTKNPSEMGRLLKAML</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LRF6</accession>
    <name>Q1LRF6_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative transcriptional regulator, TetR/AcrR-family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_0384</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07270.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0384"/>
    <dbReference type="EnsemblBacteria" id="ABF07270">
        <property type="protein sequence ID" value="ABF07270"/>
        <property type="gene ID" value="Rmet_0384"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0384"/>
    <dbReference type="eggNOG" id="COG1309">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069356_4_1_4"/>
    <dbReference type="OMA" id="VIREYHT"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001647">
        <property type="entry name" value="HTH_TetR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036271">
        <property type="entry name" value="Tet_transcr_reg_TetR-rel_C_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00440">
        <property type="entry name" value="TetR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00455">
        <property type="entry name" value="HTHTETR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48498">
        <property type="entry name" value="SSF48498"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50977">
        <property type="entry name" value="HTH_TETR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH tetR-type" evidence="2">
        <location>
            <begin position="21"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="H-T-H motif" evidence="1">
        <location>
            <begin position="44"/>
            <end position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF07270.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="212" mass="24388" checksum="34F56A6EC9504A46" modified="2006-05-30" version="1">MRHQSVRLESAAAPAPLRKGEMTRVAILDAALELSSRDGLEGLTIGLLAERMQMSKSGVFAHFGSREDLQVEVVREYHRRFEQEVFYPSLQEPRGLPRLWSMVRRWMEKRIQEVTTGCIYISGAVEYDDRAESPVRDELVKSVTIWRAALMRAIDQAKEEGHLRADCDPRLMLFEMYSLELGLHHDARFLRLPDSAELAMVALNKLIQSYRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LRH3</accession>
    <name>Q1LRH3_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Putative Fe-S oxidoreductase FAD/FMN-containing dehydrogenase oxidoreductase protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Rmet_0367</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07253.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515245.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0367"/>
    <dbReference type="EnsemblBacteria" id="ABF07253">
        <property type="protein sequence ID" value="ABF07253"/>
        <property type="gene ID" value="Rmet_0367"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0367"/>
    <dbReference type="eggNOG" id="COG0247">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG0277">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG1146">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_262983_0_0_4"/>
    <dbReference type="OMA" id="FGLMGHE"/>
    <dbReference type="OrthoDB" id="1034540at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1060.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.465.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004017">
        <property type="entry name" value="Cys_rich_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021817">
        <property type="entry name" value="DUF3400"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022153">
        <property type="entry name" value="DUF3683"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016166">
        <property type="entry name" value="FAD-bd_PCMH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036318">
        <property type="entry name" value="FAD-bd_PCMH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016169">
        <property type="entry name" value="FAD-bd_PCMH_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016164">
        <property type="entry name" value="FAD-linked_Oxase-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004113">
        <property type="entry name" value="FAD-linked_oxidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009051">
        <property type="entry name" value="Helical_ferredxn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006094">
        <property type="entry name" value="Oxid_FAD_bind_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02754">
        <property type="entry name" value="CCG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11880">
        <property type="entry name" value="DUF3400"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12447">
        <property type="entry name" value="DUF3683"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02913">
        <property type="entry name" value="FAD-oxidase_C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01565">
        <property type="entry name" value="FAD_binding_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13183">
        <property type="entry name" value="Fer4_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55103">
        <property type="entry name" value="SSF55103"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56176">
        <property type="entry name" value="SSF56176"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51387">
        <property type="entry name" value="FAD_PCMH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0408">Iron</keyword>
    <keyword evidence="3" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD-binding PCMH-type" evidence="5">
        <location>
            <begin position="187"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="4">
        <location>
            <begin position="906"/>
            <end position="935"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABF07253.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="1324" mass="147685" checksum="9C2B2ECF748396E1" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LRM8</accession>
    <name>Q1LRM8_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Putative transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">Rmet_0312</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07198.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515198.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0312"/>
    <dbReference type="EnsemblBacteria" id="ABF07198">
        <property type="protein sequence ID" value="ABF07198"/>
        <property type="gene ID" value="Rmet_0312"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0312"/>
    <dbReference type="eggNOG" id="COG1283">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025623_2_1_4"/>
    <dbReference type="OMA" id="FCAVAYN"/>
    <dbReference type="OrthoDB" id="1341748at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005436">
        <property type="term" value="F:sodium:phosphate symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044341">
        <property type="term" value="P:sodium-dependent phosphate transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.220">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003841">
        <property type="entry name" value="Na/Pi_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004633">
        <property type="entry name" value="NaPi_cotrn-rel/YqeW-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038078">
        <property type="entry name" value="PhoU-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026022">
        <property type="entry name" value="PhoU_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10010">
        <property type="entry name" value="PTHR10010"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02690">
        <property type="entry name" value="Na_Pi_cotrans"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="PhoU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00704">
        <property type="entry name" value="NaPi_cotrn_rel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="65"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="104"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="134"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="175"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="206"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="238"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="278"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="domain" description="PhoU" evidence="8">
        <location>
            <begin position="340"/>
            <end position="422"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ABF07198.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="558" mass="59452" checksum="77D4CBB564EDBC8A" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="97" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LRS5</accession>
    <name>Q1LRS5_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Cytochrome-c oxidase</fullName>
            <ecNumber evidence="3">7.1.1.9</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Cytochrome aa3 subunit 3</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">Cytochrome c oxidase polypeptide III</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="primary">coxC</name>
        <name evidence="12" type="ordered locus">Rmet_0265</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cell membrane</location>
            <topology evidence="9">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the cytochrome c oxidase subunit 3 family.</text>
    </comment>
    <dbReference type="EC" id="7.1.1.9" evidence="3"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07151.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008642679.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0265"/>
    <dbReference type="EnsemblBacteria" id="ABF07151">
        <property type="protein sequence ID" value="ABF07151"/>
        <property type="gene ID" value="Rmet_0265"/>
    </dbReference>
    <dbReference type="GeneID" id="60822990"/>
    <dbReference type="KEGG" id="rme:Rmet_0265"/>
    <dbReference type="eggNOG" id="COG1845">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_044071_0_0_4"/>
    <dbReference type="OMA" id="ALLWPDF"/>
    <dbReference type="OrthoDB" id="1502742at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004129">
        <property type="term" value="F:cytochrome-c oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019646">
        <property type="term" value="P:aerobic electron transport chain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01665">
        <property type="entry name" value="Cyt_c_Oxidase_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024791">
        <property type="entry name" value="Cyt_c/ubiquinol_Oxase_su3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033945">
        <property type="entry name" value="Cyt_c_oxase_su3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000298">
        <property type="entry name" value="Cyt_c_oxidase-like_su3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035973">
        <property type="entry name" value="Cyt_c_oxidase_su3-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013833">
        <property type="entry name" value="Cyt_c_oxidase_su3_a-hlx"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11403">
        <property type="entry name" value="PTHR11403"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00510">
        <property type="entry name" value="COX3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81452">
        <property type="entry name" value="SSF81452"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50253">
        <property type="entry name" value="COX3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="21"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="44"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="83"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="150"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="182"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="222"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="267"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="domain" description="COX3" evidence="11">
        <location>
            <begin position="7"/>
            <end position="286"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010581"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00031400"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00031625"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003376"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50253"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ABF07151.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="286" mass="32053" checksum="393EF1A44C30A6B9" modified="2006-05-30" version="1">MSANRANAPYYFVPGPSRHPITAAFGLLMTGAGAAGWVNGQPWAPYLCGFGLLWFLFVLKSWFGDAIRESEGGMYGKNIDTSFRWSMGWFIFSEVMFFAAFFGALFYARTIAMPWLGDLNNKIIWPDFAAVWPNMGPAGTVEDFQTMGPWPIPTINTALLLTSGVTLTWAHHALLAGKRSQLIQGLVLTILLGAIFMCLQAYEYMHAYSELNLKLTSGVYGSTFFLLTGFHGFHVTMGAIMLTVVLIRVLKGHFTPEHHFAFEGAAWYWHFVDVVWLGLYVVVYWL</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="70" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LS09</accession>
    <name>Q1LS09_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Rmet_0181</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07067.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515089.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0181"/>
    <dbReference type="EnsemblBacteria" id="ABF07067">
        <property type="protein sequence ID" value="ABF07067"/>
        <property type="gene ID" value="Rmet_0181"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0181"/>
    <dbReference type="eggNOG" id="COG1565">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024840_1_0_4"/>
    <dbReference type="OMA" id="YYHPQRN"/>
    <dbReference type="OrthoDB" id="1220759at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12710">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003788">
        <property type="entry name" value="NDUFAF7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038375">
        <property type="entry name" value="NDUFAF7_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12049">
        <property type="entry name" value="PTHR12049"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02636">
        <property type="entry name" value="Methyltransf_28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="4" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABF07067.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="398" mass="42739" checksum="118B940B3012BDC3" modified="2006-05-30" version="1">MQKVASLPLPSADALDASAALSICIAGAIDAAGGWIGFDRFMSLALYAPRLGYYSGGAAKFGRDVNDGSDFITAPELTPFFARTLARQFAPLVRMNLPRVMEFGAGTGRLAADLLLALETEDALPDTYQIVELSGELRARQQATLDQRAPHLAGRVTWLDALPDRFEGVVVGNEVLDAMPVRLYARTGGRWHERGVVCAKDGKAGAEAFAFEDRPLEDGAIPAVLLGIPGDHDIVTETHTEAEGFTRAVGALLARGAAFFIDYGFPASEYYHPHRTGGTLMCHYRHHAHPDPFLYPGLQDITAHVNFSGIAQAGVEAGLQVSGYASQARFLMNAGITELMMSLDPSDARAFLPQANAVQKLLSEAEMGELFKVIALTRGIDDAMPLDGFARGDRSFAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LS90</accession>
    <name>Q1LS90_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative periplasmic metal ion binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_0100</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF06986.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515021.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0100"/>
    <dbReference type="EnsemblBacteria" id="ABF06986">
        <property type="protein sequence ID" value="ABF06986"/>
        <property type="gene ID" value="Rmet_0100"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0100"/>
    <dbReference type="eggNOG" id="COG0614">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038034_2_7_4"/>
    <dbReference type="OMA" id="FSWYGSR"/>
    <dbReference type="OrthoDB" id="1473468at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002491">
        <property type="entry name" value="ABC_transptr_periplasmic_BD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01497">
        <property type="entry name" value="Peripla_BP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50983">
        <property type="entry name" value="FE_B12_PBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fe/B12 periplasmic-binding" evidence="1">
        <location>
            <begin position="18"/>
            <end position="280"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF06986.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="280" mass="30970" checksum="D182FE5E0D3E0391" modified="2006-05-30" version="1">MWTDAGGQVHEAASGDVRIASLVPSITELLFDLGLADQLVARTGFCIHPEPAVRAVAKVGGTKDVKLDRLRALAPTHVVVNIDENRRETVDEIRTFVPHVIVTHPCAPEDNLALYRLLGGIFGRRPQAEALVGQLRGELDAIQAQTWPARRVLYAIWQDPWMTVSRATYISRMLALVNWATWPDDADTSDTPAACVDGDCSRPNAPGERYPTFRWSDALVRELDAVLLSTEPYRFTEDHADALERQIGKPVLLVDGEMLSWYGSRAVAGARYLRSLAQGF</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-01-15" modified="2022-05-25" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LSC6</accession>
    <name>PXPA_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">5-oxoprolinase subunit A</fullName>
            <shortName evidence="1">5-OPase subunit A</shortName>
            <ecNumber evidence="1">3.5.2.9</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">5-oxoprolinase (ATP-hydrolyzing) subunit A</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">pxpA</name>
        <name type="ordered locus">Rmet_0064</name>
    </gene>
    <organism>
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the cleavage of 5-oxoproline to form L-glutamate coupled to the hydrolysis of ATP to ADP and inorganic phosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>5-oxo-L-proline + ATP + 2 H2O = ADP + H(+) + L-glutamate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:10348"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58402"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.5.2.9"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Forms a complex composed of PxpA, PxpB and PxpC.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the LamB/PxpA family.</text>
    </comment>
    <dbReference type="EC" id="3.5.2.9" evidence="1"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF06950.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011514989.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q1LSC6"/>
    <dbReference type="SMR" id="Q1LSC6"/>
    <dbReference type="STRING" id="266264.Rmet_0064"/>
    <dbReference type="EnsemblBacteria" id="ABF06950">
        <property type="protein sequence ID" value="ABF06950"/>
        <property type="gene ID" value="Rmet_0064"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0064"/>
    <dbReference type="eggNOG" id="COG1540">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069535_0_0_4"/>
    <dbReference type="OMA" id="DRTYKQD"/>
    <dbReference type="OrthoDB" id="918580at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017168">
        <property type="term" value="F:5-oxoprolinase (ATP-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00691">
        <property type="entry name" value="PxpA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011330">
        <property type="entry name" value="Glyco_hydro/deAcase_b/a-brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005501">
        <property type="entry name" value="LamB/YcsF/PxpA-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30292">
        <property type="entry name" value="PTHR30292"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03746">
        <property type="entry name" value="LamB_YcsF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88713">
        <property type="entry name" value="SSF88713"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_1000045217" description="5-oxoprolinase subunit A">
        <location>
            <begin position="1"/>
            <end position="246"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00691"/>
        </source>
    </evidence>
    <sequence length="246" mass="26563" checksum="925D5BDBEC3CCB58" modified="2006-05-30" version="1">MQIDLNADLGEGCGNDEALLALISSANIACGWHAGDAATMLQTVKWALANKVSIGAHPSFPDRENFGRTEMQRDPEAVYADVLYQIGALDAMVRAQGGELAHVKPHGALYNMAVRDAKLCEAIVRAVRDYDSDLVFFGLANSQMIDIARAAGLRVKEEVFADRGYNPDGTLVKRGTPGALHEDEDVALNQTLSMVRDKQVRAIDGTWVPIRAETVCLHGDGAHALAFARRIRERLGAEGIAIRAGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="110" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LSE9</accession>
    <name>Q1LSE9_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Histidine kinase</fullName>
            <ecNumber evidence="3">2.7.13.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">kdpD</name>
        <name evidence="10" type="ordered locus">Rmet_0041</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.</text>
            <dbReference type="EC" id="2.7.13.3"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.7.13.3" evidence="3"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF06927.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011514968.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0041"/>
    <dbReference type="EnsemblBacteria" id="ABF06927">
        <property type="protein sequence ID" value="ABF06927"/>
        <property type="gene ID" value="Rmet_0041"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0041"/>
    <dbReference type="eggNOG" id="COG2205">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_113_1_4"/>
    <dbReference type="OMA" id="LWATAFQ"/>
    <dbReference type="OrthoDB" id="1293707at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00082">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003018">
        <property type="entry name" value="GAF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029016">
        <property type="entry name" value="GAF-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003661">
        <property type="entry name" value="HisK_dim/P"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036097">
        <property type="entry name" value="HisK_dim/P_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038318">
        <property type="entry name" value="KdpD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025201">
        <property type="entry name" value="KdpD_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004358">
        <property type="entry name" value="Sig_transdc_His_kin-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003852">
        <property type="entry name" value="Sig_transdc_His_kinase_KdpD_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13493">
        <property type="entry name" value="DUF4118"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13492">
        <property type="entry name" value="GAF_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00512">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02702">
        <property type="entry name" value="KdpD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00344">
        <property type="entry name" value="BCTRLSENSOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00388">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47384">
        <property type="entry name" value="SSF47384"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="SSF55874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="10" id="KW-0407">Ion channel</keyword>
    <keyword evidence="10" id="KW-0406">Ion transport</keyword>
    <keyword evidence="6 10" id="KW-0418">Kinase</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="10" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="483"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="506"/>
            <end position="523"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="535"/>
            <end position="556"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="9">
        <location>
            <begin position="736"/>
            <end position="950"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="947"/>
            <end position="967"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ABF06927.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="967" mass="106179" checksum="38EF002956EE1C08" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-10-07" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LSF3</accession>
    <name>Q1LSF3_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_0037</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF06923.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0037"/>
    <dbReference type="EnsemblBacteria" id="ABF06923">
        <property type="protein sequence ID" value="ABF06923"/>
        <property type="gene ID" value="Rmet_0037"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0037"/>
    <dbReference type="HOGENOM" id="CLU_3065365_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="32"/>
            <end position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF06923.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="53" mass="5202" checksum="D2990E970A55E277" modified="2010-03-23" version="2">MDGIYFLVLLAFGALTGALLALCAALGANPPASRGNGEAAAADSRPAADNRGQ</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="128" xmlns="http://uniprot.org/uniprot">
    <accession>Q44006</accession>
    <accession>Q58AM1</accession>
    <name>CZCR_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName>Transcriptional activator protein CzcR</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">czcR</name>
        <name type="ordered locus">Rmet_5978</name>
    </gene>
    <organism>
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid">
        <name>pMOL30</name>
    </geneLocation>
    <reference key="1">
        <citation type="journal article" date="1997" name="Mol. Microbiol." volume="23" first="493" last="503">
            <title>Two-component regulatory system involved in transcriptional control of heavy-metal homoeostasis in Alcaligenes eutrophus.</title>
            <authorList>
                <person name="van der Lelie D."/>
                <person name="Schwuchow T."/>
                <person name="Schwidetzky T."/>
                <person name="Wuertz S."/>
                <person name="Baeyens W."/>
                <person name="Mergeay M."/>
                <person name="Nies D.H."/>
            </authorList>
            <dbReference type="PubMed" id="9044283"/>
            <dbReference type="DOI" id="10.1046/j.1365-2958.1997.d01-1866.x"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC DNA]</scope>
    </reference>
    <reference key="2">
        <citation type="submission" date="2004-11" db="EMBL/GenBank/DDBJ databases">
            <title>Sequence and features of the Ralstonia metallidurans CH34 heavy metals plasmids pMOL28 and pMOL30.</title>
            <authorList>
                <person name="Monchy S."/>
                <person name="van der Lelie D."/>
                <person name="Vallaeys T."/>
                <person name="Taghavi S."/>
                <person name="Benotmane M."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="Lapidus A."/>
                <person name="Mergeay M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference key="3">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text>Member of the two-component regulatory system CzcS/CzcR involved in the control of cobalt, zinc and cadmium homeostasis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="induction">
        <text>By cadmium, lead, and zinc.</text>
    </comment>
    <comment type="PTM">
        <text evidence="3">Phosphorylated by CzcS.</text>
    </comment>
    <dbReference type="EMBL" id="X71400">
        <property type="protein sequence ID" value="CAI11246.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="X98451">
        <property type="protein sequence ID" value="CAA67086.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP000354">
        <property type="protein sequence ID" value="ABF12837.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011229348.1">
        <property type="nucleotide sequence ID" value="NC_007971.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_145597.1">
        <property type="nucleotide sequence ID" value="NC_006466.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q44006"/>
    <dbReference type="SMR" id="Q44006"/>
    <dbReference type="EnsemblBacteria" id="ABF12837">
        <property type="protein sequence ID" value="ABF12837"/>
        <property type="gene ID" value="Rmet_5978"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5978"/>
    <dbReference type="HOGENOM" id="CLU_000445_30_1_4"/>
    <dbReference type="OMA" id="EFSILWV"/>
    <dbReference type="OrthoDB" id="1020672at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid pMOL30"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00383">
        <property type="entry name" value="trans_reg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001867">
        <property type="entry name" value="OmpR/PhoB-type_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006291">
        <property type="entry name" value="PcoR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039420">
        <property type="entry name" value="WalR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48111">
        <property type="entry name" value="PTHR48111"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00486">
        <property type="entry name" value="Trans_reg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00862">
        <property type="entry name" value="Trans_reg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01387">
        <property type="entry name" value="cztR_silR_copR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51755">
        <property type="entry name" value="OMPR_PHOB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword id="KW-0010">Activator</keyword>
    <keyword id="KW-0104">Cadmium</keyword>
    <keyword id="KW-0170">Cobalt</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0238">DNA-binding</keyword>
    <keyword id="KW-0597">Phosphoprotein</keyword>
    <keyword id="KW-0614">Plasmid</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0804">Transcription</keyword>
    <keyword id="KW-0805">Transcription regulation</keyword>
    <keyword id="KW-0902">Two-component regulatory system</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <feature type="chain" id="PRO_0000081087" description="Transcriptional activator protein CzcR">
        <location>
            <begin position="1"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="domain" description="Response regulatory" evidence="1">
        <location>
            <begin position="2"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="OmpR/PhoB-type" evidence="2">
        <location>
            <begin position="123"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="1">
        <location>
            <position position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="225" mass="25540" checksum="8ABDDFF2B140A017" modified="1996-11-01" version="1">MRVLVVEDEPRTAEYLQKGLSESGFVVDIANNGGDGLHMAEETDYDVIILDVMLPGMDGWTVIKSIRSKSETPVLFLTALDDVADRVRGFELGADDYLVKPFAFAELLARIRRCLRQSTSKESERLRIADLDIDVLGRRVFRGTTRIELTNQEFSLLHLLMRRRGEVLSRTTIASQVWGVNFDTDTNVVDVAIRRLRSKVDDPFDQKLIHTVRGMGYVLDPERGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-03-23" modified="2020-10-07" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>D3DXU9</accession>
    <name>D3DXU9_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_6511</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ADC45119.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADC45119">
        <property type="protein sequence ID" value="ADC45119"/>
        <property type="gene ID" value="Rmet_6511"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6511"/>
    <dbReference type="HOGENOM" id="CLU_2772945_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADC45119.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="69" mass="7392" checksum="CCEA66D7381FA3B8" modified="2010-03-23" version="1">MQYSHGPGPDYHNYGETAPRAMRLGWGTPEPCPARHAVPRRLGSRTCGKQNMTACRGPESEGAPLPCGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-03-23" modified="2020-10-07" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>D3DXX7</accession>
    <name>D3DXX7_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_6540</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ADC45147.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_6540"/>
    <dbReference type="EnsemblBacteria" id="ADC45147">
        <property type="protein sequence ID" value="ADC45147"/>
        <property type="gene ID" value="Rmet_6540"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6540"/>
    <dbReference type="HOGENOM" id="CLU_2882719_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADC45147.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="63" mass="7180" checksum="3DC1DBDF48E898E8" modified="2010-03-23" version="1">MSRITIDLPYIRLGNVEAFIERVSPRKRGSWFNLQRDTSGFHLGGLWLDVQVNHCARPRGNAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-03-23" modified="2020-10-07" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>D3DY75</accession>
    <name>D3DY75_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_6645</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ADC45245.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_6645"/>
    <dbReference type="EnsemblBacteria" id="ADC45245">
        <property type="protein sequence ID" value="ADC45245"/>
        <property type="gene ID" value="Rmet_6645"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6645"/>
    <dbReference type="HOGENOM" id="CLU_2411059_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADC45245.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="92" mass="10327" checksum="67000080801C6766" modified="2010-03-23" version="1">MRRCERQSASRLRLRPECMVAAPSGSDWDLDFLMSPIENIHFDPGRHSVKIVHLDRPKGMRTRWDYAADESAACRLPPTVGPRAAAAARLPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-03-23" modified="2020-10-07" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>D3DY98</accession>
    <name>D3DY98_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_6680</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ADC45268.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_6680"/>
    <dbReference type="EnsemblBacteria" id="ADC45268">
        <property type="protein sequence ID" value="ADC45268"/>
        <property type="gene ID" value="Rmet_6680"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6680"/>
    <dbReference type="HOGENOM" id="CLU_2957387_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADC45268.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="59" mass="6584" checksum="D28D16B6BA071B1A" modified="2010-03-23" version="1">MKNALFIPQIGIFSGVSLLILTQGRRTRGVTMTKTWKAEPGGRRCLKRTEVEARNAPLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-06-17" version="52" xmlns="http://uniprot.org/uniprot">
    <accession>Q1L9W9</accession>
    <name>Q1L9W9_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Protein (Secreted)corresponding to a gene involved in the response to chromate</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">chrO</name>
        <name evidence="2" type="ordered locus">Rmet_6198</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2 3">
        <name>pMOL28</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid pMOL28</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000355">
        <property type="protein sequence ID" value="ABF13057.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF13057">
        <property type="protein sequence ID" value="ABF13057"/>
        <property type="gene ID" value="Rmet_6198"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6198"/>
    <dbReference type="HOGENOM" id="CLU_159283_0_0_4"/>
    <dbReference type="OMA" id="TDEVHWA"/>
    <dbReference type="OrthoDB" id="1952976at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid pMOL28"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193226" evidence="1">
        <location>
            <begin position="21"/>
            <end position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF13057.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="119" mass="13075" checksum="95FF06F68EA4E3BF" modified="2006-05-30" version="1">MKRILTAVCAGIAITSSATAATAQDVFRHLKASEIKRTISGKVITDGPHWADKFASDGTVESIMQGQVQKGRWSVRGSNLCLAYPSAKAEECFEVWRYGQMIEYRRDGVLLAQGKLVIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="53" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LA83</accession>
    <name>Q1LA83_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_6084</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="1 2">
        <name>pMOL30</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid pMOL30</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000354">
        <property type="protein sequence ID" value="ABF12943.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011514857.1">
        <property type="nucleotide sequence ID" value="NC_007971.2"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF12943">
        <property type="protein sequence ID" value="ABF12943"/>
        <property type="gene ID" value="Rmet_6084"/>
    </dbReference>
    <dbReference type="GeneID" id="60825037"/>
    <dbReference type="KEGG" id="rme:Rmet_6084"/>
    <dbReference type="HOGENOM" id="CLU_1377287_0_0_4"/>
    <dbReference type="OMA" id="CKSWQQG"/>
    <dbReference type="OrthoDB" id="1485385at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid pMOL30"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF12943.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="206" mass="23386" checksum="5F9DDC72BD43CA02" modified="2006-05-30" version="1">MKEDILEQMVDEYLQHKGYFTQHNLKFRPAKDHVDYVAQADAVHSDVDVIGIHPLMQGPERVVVVSCKSWQQGFSPQYWSDAIAKNKKVNGREAWMAFRELARPKWAQAFRAEVERATGAKAFTYVTAVTRLTAQADRTAWEQHPEFRQSLNGNPIRILTFDDMLSELFPTINQTVASSQLGRLLQLIKASGWALASRNENGPSLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LAB7</accession>
    <name>Q1LAB7_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_6050</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2 3">
        <name>pMOL30</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid pMOL30</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000354">
        <property type="protein sequence ID" value="ABF12909.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF12909">
        <property type="protein sequence ID" value="ABF12909"/>
        <property type="gene ID" value="Rmet_6050"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6050"/>
    <dbReference type="HOGENOM" id="CLU_2143768_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid pMOL30"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="47"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="88"/>
            <end position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF12909.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="112" mass="12274" checksum="D3B933C7ABA29194" modified="2006-05-30" version="1">MSTDPVTIGTTTEIHEYAFDVRLSCAVRVKATSKAEAIKLLDRIQGNSANLGSWPNGDPGTHGRSLRGNREISRLASRSMDWSASGRRGAVADDERTGEVRLRRSSEERSTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LAN8</accession>
    <name>Q1LAN8_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Rmet_5929</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="9">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="9">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the UPF0410 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12788.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518135.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5929"/>
    <dbReference type="EnsemblBacteria" id="ABF12788">
        <property type="protein sequence ID" value="ABF12788"/>
        <property type="gene ID" value="Rmet_5929"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5929"/>
    <dbReference type="eggNOG" id="COG2261">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_160040_2_0_4"/>
    <dbReference type="OMA" id="GWIMTIV"/>
    <dbReference type="OrthoDB" id="2084822at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3470.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037294">
        <property type="entry name" value="ABC_BtuC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007341">
        <property type="entry name" value="Transgly_assoc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33884">
        <property type="entry name" value="PTHR33884"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04226">
        <property type="entry name" value="Transgly_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0614">Plasmid</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="30"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="60"/>
            <end position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ABF12788.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="83" mass="8693" checksum="B9F22D540B0534DA" modified="2006-05-30" version="1">MMAFLGTVFVGLIVGLIARAIKPGDDKMGWIMTIVLGILGSVAAGYVGRALGMYQPGQPAGWIASVIGAIVLLAIYDMVRRKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LAV7</accession>
    <name>Q1LAV7_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 4">GDP-mannose 4,6-dehydratase</fullName>
            <ecNumber evidence="3 4">4.2.1.47</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">GDP-D-mannose dehydratase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="4 6" type="primary">gmd</name>
        <name evidence="6" type="ordered locus">Rmet_5860</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="7">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="7">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Catalyzes the conversion of GDP-D-mannose to GDP-4-dehydro-6-deoxy-D-mannose.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>GDP-alpha-D-mannose = GDP-4-dehydro-alpha-D-rhamnose + H2O</text>
            <dbReference type="Rhea" id="RHEA:23820"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:57527"/>
            <dbReference type="ChEBI" id="CHEBI:57964"/>
            <dbReference type="EC" id="4.2.1.47"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 4">
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 4">Belongs to the NAD(P)-dependent epimerase/dehydratase family. GDP-mannose 4,6-dehydratase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.47" evidence="3 4"/>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12719.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5860"/>
    <dbReference type="EnsemblBacteria" id="ABF12719">
        <property type="protein sequence ID" value="ABF12719"/>
        <property type="gene ID" value="Rmet_5860"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5860"/>
    <dbReference type="eggNOG" id="COG1089">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007383_14_0_4"/>
    <dbReference type="OMA" id="TDCLYLG"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008446">
        <property type="term" value="F:GDP-mannose 4,6-dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070401">
        <property type="term" value="F:NADP+ binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019673">
        <property type="term" value="P:GDP-mannose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00955">
        <property type="entry name" value="GDP_Man_dehydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006368">
        <property type="entry name" value="GDP_Man_deHydtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016040">
        <property type="entry name" value="NAD(P)-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43715">
        <property type="entry name" value="PTHR43715"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16363">
        <property type="entry name" value="GDP_Man_Dehyd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01472">
        <property type="entry name" value="gmd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0456">Lyase</keyword>
    <keyword evidence="4" id="KW-0521">NADP</keyword>
    <keyword evidence="6" id="KW-0614">Plasmid</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAD(P)-bd_dom" evidence="5">
        <location>
            <begin position="32"/>
            <end position="375"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00955"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF16363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABF12719.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="401" mass="45574" checksum="E8AEC1FB3DD733CF" modified="2006-05-30" version="1">MGVNELDNLFDAADKPGDASTPRGANGRRRALITGITGQDGSYLCEYLLDKGYEVHGIKRRSSLFNTERIDHLYEDPQAPDRRLILHHGDMTDTASLVRVVQQSRPDEVYNLAAQSHVQVSFEEPEYTANTDAVGTLRLLEAMRILGMEQSTRFYQASTSELYGLVQEIPQKETTPFYPRSPYAVAKLYAYWITVNYREAYGMYACNGILFNHESPVRGETFVTRKITRAIARIALGLQEVLYLGNLSALRDWGHARDYVEMQWLMLQQETPRDFVIATGEQHSVREFVTLSAQKLGLTVTFEGSGVDEVGIISHIDTSRRELSSRCKVGDVIVRVDPRYFRPTEVETLLGDPTQAREQLGWSPRITFDELVHEMIEADFVAARRDALVKMAGFRTYDYHE</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LB49</accession>
    <name>Q1LB49_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">AMP-dependent synthetase and ligase putative o-succinylbenzoate-CoA ligase</fullName>
            <ecNumber evidence="3">6.2.1.26</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_5768</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EC" id="6.2.1.26" evidence="3"/>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12627.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011520167.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5768"/>
    <dbReference type="EnsemblBacteria" id="ABF12627">
        <property type="protein sequence ID" value="ABF12627"/>
        <property type="gene ID" value="Rmet_5768"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5768"/>
    <dbReference type="eggNOG" id="COG0318">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000022_59_5_4"/>
    <dbReference type="OMA" id="ITHRNIW"/>
    <dbReference type="OrthoDB" id="377638at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008756">
        <property type="term" value="F:o-succinylbenzoate-CoA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025110">
        <property type="entry name" value="AMP-bd_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045851">
        <property type="entry name" value="AMP-bd_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020845">
        <property type="entry name" value="AMP-binding_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_Synth/Lig"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13193">
        <property type="entry name" value="AMP-binding_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00455">
        <property type="entry name" value="AMP_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0436">Ligase</keyword>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AMP-binding" evidence="1">
        <location>
            <begin position="26"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="domain" description="AMP-binding_C" evidence="2">
        <location>
            <begin position="452"/>
            <end position="526"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF12627.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="544" mass="59337" checksum="1829998B1191C82D" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LB71</accession>
    <name>Q1LB71_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 4">Ferric uptake regulation protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4 5" type="primary">fur</name>
        <name evidence="5" type="ordered locus">Rmet_5746</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="6">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="6">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="4">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the Fur family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12605.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011520147.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5746"/>
    <dbReference type="EnsemblBacteria" id="ABF12605">
        <property type="protein sequence ID" value="ABF12605"/>
        <property type="gene ID" value="Rmet_5746"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5746"/>
    <dbReference type="eggNOG" id="COG0735">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_096072_3_3_4"/>
    <dbReference type="OMA" id="HHYHLIC"/>
    <dbReference type="OrthoDB" id="1630127at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd07153">
        <property type="entry name" value="Fur_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.190">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002481">
        <property type="entry name" value="FUR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043135">
        <property type="entry name" value="Fur_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33202">
        <property type="entry name" value="PTHR33202"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01475">
        <property type="entry name" value="FUR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0614">Plasmid</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0678">Repressor</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="3 4" id="KW-0862">Zinc</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007957"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00020910"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU364037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABF12605.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="157" mass="17853" checksum="941140F2BD3DAEE2" modified="2006-05-30" version="1">MMTSNVNADVVPLKRAGLRATSPRMHVLEVFRASERRHLSAEDVYRTMLELGIDAGLSTVYRVLNQLVQADILLRHSFESDHSVFELNEGGHHDHLICLECGRVEEFRDEAIEQRQKQVAARNDFVLREHMLVLYGLCPDCQRSRIGAVEADVDAAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-12-02" version="60" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LBE2</accession>
    <name>Q1LBE2_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Sulf_transp domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">Rmet_5675</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="10">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="10">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the UPF0394 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12534.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5675"/>
    <dbReference type="EnsemblBacteria" id="ABF12534">
        <property type="protein sequence ID" value="ABF12534"/>
        <property type="gene ID" value="Rmet_5675"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5675"/>
    <dbReference type="eggNOG" id="COG2391">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_122700_1_0_4"/>
    <dbReference type="OMA" id="IDWANFT"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007272">
        <property type="entry name" value="Sulf_transp"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04143">
        <property type="entry name" value="Sulf_transp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0614">Plasmid</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="53"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="81"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="122"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="domain" description="Sulf_transp" evidence="8">
        <location>
            <begin position="51"/>
            <end position="133"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009590"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF04143"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ABF12534.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="145" mass="14676" checksum="06F01211E08F1C3D" modified="2006-05-30" version="1">MLLIDLGNFTPGLSLAGGIVIGVAAAVLVLFNGRIAGISGILGGLLSLPRRDMTWRVAFLIGLIGAPILAALFGMPAKADIQASWGEVLVAGFLVGIGTRYAGGCTSGHGVCGISRGSIRSLVATLTFMAAGFLTVFVTHHLIGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="114" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LBE4</accession>
    <name>Q1LBE4_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Sensor protein</fullName>
            <ecNumber evidence="5">2.7.13.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">copS2</name>
        <name evidence="8" type="ordered locus">Rmet_5673</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="9">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="9">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Member of a two-component regulatory system.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.</text>
            <dbReference type="EC" id="2.7.13.3"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cell inner membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.7.13.3" evidence="5"/>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12532.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011520078.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5673"/>
    <dbReference type="EnsemblBacteria" id="ABF12532">
        <property type="protein sequence ID" value="ABF12532"/>
        <property type="gene ID" value="Rmet_5673"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5673"/>
    <dbReference type="eggNOG" id="COG2205">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_89_6_4"/>
    <dbReference type="OMA" id="MPAQQNE"/>
    <dbReference type="OrthoDB" id="692375at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00082">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006290">
        <property type="entry name" value="CztS_silS_copS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003660">
        <property type="entry name" value="HAMP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003661">
        <property type="entry name" value="HisK_dim/P"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036097">
        <property type="entry name" value="HisK_dim/P_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004358">
        <property type="entry name" value="Sig_transdc_His_kin-like_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00672">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00512">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00344">
        <property type="entry name" value="BCTRLSENSOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00304">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00388">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47384">
        <property type="entry name" value="SSF47384"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="SSF55874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01386">
        <property type="entry name" value="cztS_silS_copS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50885">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4 5 8" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="8" id="KW-0614">Plasmid</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0902">Two-component regulatory system</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="164"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="domain" description="HAMP" evidence="7">
        <location>
            <begin position="184"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="6">
        <location>
            <begin position="245"/>
            <end position="459"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU364088"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ABF12532.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="462" mass="50927" checksum="440661B737942E5D" modified="2006-05-30" version="1">MRGYSLTTRLTALFSLTSACVLLGLGVLIFTAMDRHFAVEDYALLRDNMRLVEKTIADNTGSTLPTRLADAYQHHSGLLVYTRGANGQVHYATPDFDFAAALKTSIEASRKHVGDDDVFQWTQDGKTYRGMRATVNAAGELTDVLLGMDTEIHEHFVHAFRRSLVSYVALAVLASGVFGWWAARRGLAPLRAMATRAKVVTGDKLHERMPVEAVPEEVANLAANLNAMLERLQDDFRRLSEFSSDLAHELRTPLTNLMTQTQVALSQPRDAEKYREILASNAEELQRLSRMVSDMLYLAKMEHGLDLPHKETISIAQEAGALFEFYEALAEDKQVALRLQGDGIVEGDRLMLRRALSNLLSNALRHTPQAGAVVVEAHAEGDSVVVAVENDGPEIPAELLSSLFDRFFRGDKSRRRPESDSVGLGLSITRAIMTAHGGGISVQSANGKTRFVLRFPPRNAQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="52" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LBQ1</accession>
    <name>Q1LBQ1_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_5566</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12425.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519975.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5566"/>
    <dbReference type="EnsemblBacteria" id="ABF12425">
        <property type="protein sequence ID" value="ABF12425"/>
        <property type="gene ID" value="Rmet_5566"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5566"/>
    <dbReference type="HOGENOM" id="CLU_1234187_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF12425.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="224" mass="24756" checksum="7D733B94937E4225" modified="2006-05-30" version="1">MSANLPTLRKTMPAVAATEFPEFAYLVSDFGHFAPRGSEYGVQAPAGISIYTAIVRVPITASYVHCIPEDADIVGLKFEAIQGKYGRVLGIRFQFERLEVSVFAAMDDSAASSAVLDWRHSGIVPFIFGTQHGEVWRYHLKTCPFSEDDWAEASRVLLAPENQGQQPEKTWDDMCRLADEQTAVVPADAISEGEDVMHTLGFPHNRPLSFAGIFPIKQRHIGVC</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="95" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LC43</accession>
    <name>Q1LC43_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Transcriptional regulator, LysR family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_5424</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the LysR transcriptional regulatory family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12283.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5424"/>
    <dbReference type="EnsemblBacteria" id="ABF12283">
        <property type="protein sequence ID" value="ABF12283"/>
        <property type="gene ID" value="Rmet_5424"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5424"/>
    <dbReference type="eggNOG" id="COG0583">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039613_37_1_4"/>
    <dbReference type="OMA" id="GESRWLF"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005119">
        <property type="entry name" value="LysR_subst-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000847">
        <property type="entry name" value="Tscrpt_reg_HTH_LysR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00126">
        <property type="entry name" value="HTH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03466">
        <property type="entry name" value="LysR_substrate"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00039">
        <property type="entry name" value="HTHLYSR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50931">
        <property type="entry name" value="HTH_LYSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH lysR-type" evidence="2">
        <location>
            <begin position="5"/>
            <end position="62"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF12283.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="304" mass="34207" checksum="2300EA34B5038183" modified="2006-05-30" version="1">MMKIPPLNPLKVFEVVARAKNLTTAANELRVSQSAVSRQIAVLEEYLGVQLFTRERIGVKLTEVGEAYARRIGPAFEEISEATKFVTQKYSDNVIRLRTYTTLTARWLIPRLPHFKLQYPDVEVLITNSNAPLDFGTEQCDLAIVLGDGNWPNAEATLLLEDVIEPVCSPAFLEAFHGSTEQLLESLQSKRLLVSKYRKNDWPTWLAHVGMGHVFDKAEKMVFSSSILTWQAATDNLGIAIGQQHMLDPDLKAGRLVCPFARPLRTGRDHYIVTPSVQRYSGKIAAFKEWLVREARESKPMTPG</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LC45</accession>
    <name>Q1LC45_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Sulfatase-modifying factor 1 (C-alpha-formyglycine-generating enzyme 1) putative exported protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">sumF</name>
        <name evidence="2" type="ordered locus">Rmet_5422</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12281.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519829.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5422"/>
    <dbReference type="EnsemblBacteria" id="ABF12281">
        <property type="protein sequence ID" value="ABF12281"/>
        <property type="gene ID" value="Rmet_5422"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5422"/>
    <dbReference type="eggNOG" id="COG1262">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012431_4_0_4"/>
    <dbReference type="OMA" id="EWEYAGK"/>
    <dbReference type="OrthoDB" id="1377603at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1580.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016187">
        <property type="entry name" value="CTDL_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005532">
        <property type="entry name" value="SUMF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042095">
        <property type="entry name" value="SUMF_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03781">
        <property type="entry name" value="FGE-sulfatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56436">
        <property type="entry name" value="SSF56436"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FGE-sulfatase" evidence="1">
        <location>
            <begin position="69"/>
            <end position="348"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF12281.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="352" mass="37342" checksum="AE0F95181243CC32" modified="2006-05-30" version="1">MVVNVLRNRGRWRTPLAVAGLAFAVGVIASLGVHAVPGAAMPDGATLGSVQRCAAYSGLPAGWGKSRTAGMARVTGGEFVPGTTLGYPDERPAGKTRVGSFWIDRTEVTVAQFAAFVQATGYVTDAERQGAAVVFHKPTDAELGQRPYAWWTMVTGANWRHPEGPAAANSHGYDHRRDNQPVTLVTQADARAYANWLGHDLPTEDEWEFAAKAGRSDAGLETAPQTAEGTPTANYWQGVFPVLNTSRDGFAGLAPVGCYTANALGLFDMIANAWEWTGDAYTGPRQSHANGDTAVVAAASRSRKPAATSVIKGGSFLCAPDFCVRYRASARESAESDLPTSHIGFRTVLRDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="62" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LC80</accession>
    <name>Q1LC80_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_5387</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12246.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519792.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5387"/>
    <dbReference type="EnsemblBacteria" id="ABF12246">
        <property type="protein sequence ID" value="ABF12246"/>
        <property type="gene ID" value="Rmet_5387"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5387"/>
    <dbReference type="eggNOG" id="COG3247">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031762_0_0_4"/>
    <dbReference type="OMA" id="IFGGWNM"/>
    <dbReference type="OrthoDB" id="1137170at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="20"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="51"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="77"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="99"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="131"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="157"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="187"/>
            <end position="214"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF12246.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="440" mass="48705" checksum="8784C56A43F419BF" modified="2006-05-30" version="1">MVRLLMLLLGVDYLRTRWKALAWAGALSIVVGAVLFIDALDGKLDFPIQPFAWLLLLEGLATLAVASTGIGGQRTLRYAKGIAFSVAAILVLAERHHGHMVLSVIFGALFLADGLLQVASAHVVRFRTWRLAMAGGVIEIALAIFFIQPYPTHYVGTVPYCVALGLIFGGWNMLLLASRARRLAGNPGLRKDPESASAESNKRRLTTWSGPPASDERALTVHVWTPVGSAKSKAQHQPIIDRYIAAVDRDGVISTGHAALESPEGIYISLYPAVEIDRSPDDFTRLLRATQENNVPGRFQPDYPTESKAWCASTVQVRIRNYDAERLQNFWHDYRQNTTYNLTYRNCSSTVARALDAALEGAATRAWTRLGGWWPLFRLLLTPELWVAAHLRKRAATMAWTPGLTLDYARAMSMLADPRPFAWATVWSGLAARRSGRESR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="109" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LCF7</accession>
    <name>Q1LCF7_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Two component transcriptional regulator, LuxR family (Receiver domain CheY-like)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Rmet_5310</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="6">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="6">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12169.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519714.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5310"/>
    <dbReference type="EnsemblBacteria" id="ABF12169">
        <property type="protein sequence ID" value="ABF12169"/>
        <property type="gene ID" value="Rmet_5310"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5310"/>
    <dbReference type="eggNOG" id="COG4566">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_90_4_4"/>
    <dbReference type="OMA" id="KMNKHIA"/>
    <dbReference type="OrthoDB" id="930905at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06170">
        <property type="entry name" value="LuxR_C_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000792">
        <property type="entry name" value="Tscrpt_reg_LuxR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00196">
        <property type="entry name" value="GerE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00038">
        <property type="entry name" value="HTHLUXR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00421">
        <property type="entry name" value="HTH_LUXR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50043">
        <property type="entry name" value="HTH_LUXR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="5" id="KW-0614">Plasmid</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Response regulatory" evidence="4">
        <location>
            <begin position="18"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="domain" description="HTH luxR-type" evidence="3">
        <location>
            <begin position="148"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="2">
        <location>
            <position position="67"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABF12169.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="228" mass="24363" checksum="2000A30892CD0503" modified="2006-05-30" version="1">MTSTCLEPAASAEYEPGTVLVLDDDAMVLDALGGLFRSLDMDVALFASEAELMAYPLPDTPCCLLLDVRLGGASGLDLQSRLGQSGVGMPIIFMTGYGDVAMTAAAMKAGAVDFLTKPILETSLIAAVSAALDRDRSRRRGMRRTDAIRRGFATLTPREAEVMGMAVSGLLNKQIAGEIGISEVTVKIHRGQAMRKMNARSFADLVLMGQELGLYRDAARRARQFADA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LCK0</accession>
    <name>Q1LCK0_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative alpha/beta hydrolase putative prolyl aminopeptidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_5267</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12126.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519672.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5267"/>
    <dbReference type="ESTHER" id="ralme-q1lck0">
        <property type="family name" value="AlphaBeta_hydrolase"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF12126">
        <property type="protein sequence ID" value="ABF12126"/>
        <property type="gene ID" value="Rmet_5267"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5267"/>
    <dbReference type="eggNOG" id="COG2267">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020336_2_0_4"/>
    <dbReference type="OMA" id="HYQYSFQ"/>
    <dbReference type="OrthoDB" id="1119700at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004177">
        <property type="term" value="F:aminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000639">
        <property type="entry name" value="Epox_hydrolase-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12697">
        <property type="entry name" value="Abhydrolase_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00111">
        <property type="entry name" value="ABHYDROLASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00412">
        <property type="entry name" value="EPOXHYDRLASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="SSF53474"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0031">Aminopeptidase</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193310" evidence="1">
        <location>
            <begin position="22"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="domain" description="AB hydrolase-1" evidence="2">
        <location>
            <begin position="76"/>
            <end position="331"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF12126.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="344" mass="37983" checksum="CC1C8F7B6709FBE4" modified="2006-05-30" version="1">MLKVRPLLLAAIALSVPLAQAAHAAQTADSGPAYGPQLEGFDYPYPVQQYRFSSQGQPLEMAYIDVKPEKPNGRAVVLLHGKNFCAATWQESIQQLTAAGYRVIAPDQIGFCKSTKPPHYQYSFQQLARNTHDLLASIGVHDVTVIGHSTGGMLAMRYALMYPKETAQLVLVNPIGLEDWKAKGVPSLSVDQWYARELNVTADRIRNYEKSTYYVNQWKPEYEPWVQMLAGMNRGPGKQIVAWNSALLYDMIYTQPVVYEMSAIQAPTLLMIGDRDTTAIAKDAAPPDVQAKLGHYPELAKATARAIPNTTLVEFPDMGHAPQMQDPQAFHKALLEGLAKVGGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="104" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LCK3</accession>
    <name>Q1LCK3_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Flagellum-specific ATP synthase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">fliI</name>
        <name evidence="10" type="ordered locus">Rmet_5264</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="11">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="11">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12123.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519669.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5264"/>
    <dbReference type="EnsemblBacteria" id="ABF12123">
        <property type="protein sequence ID" value="ABF12123"/>
        <property type="gene ID" value="Rmet_5264"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5264"/>
    <dbReference type="eggNOG" id="COG1157">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022398_5_3_4"/>
    <dbReference type="OMA" id="MLMMDSV"/>
    <dbReference type="OrthoDB" id="875807at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045261">
        <property type="term" value="C:proton-transporting ATP synthase complex, catalytic core F(1)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030257">
        <property type="term" value="C:type III protein secretion system complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046933">
        <property type="term" value="F:proton-transporting ATP synthase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046961">
        <property type="term" value="F:proton-transporting ATPase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044780">
        <property type="term" value="P:bacterial-type flagellum assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071973">
        <property type="term" value="P:bacterial-type flagellum-dependent cell motility"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030254">
        <property type="term" value="P:protein secretion by the type III secretion system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020003">
        <property type="entry name" value="ATPase_a/bsu_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000194">
        <property type="entry name" value="ATPase_F1/V1/A1_a/bsu_nucl-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005714">
        <property type="entry name" value="ATPase_T3SS_FliI/YscN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020005">
        <property type="entry name" value="FliI_clade1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040627">
        <property type="entry name" value="T3SS_ATPase_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00006">
        <property type="entry name" value="ATP-synt_ab"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18269">
        <property type="entry name" value="T3SS_ATPase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03496">
        <property type="entry name" value="FliI_clade1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01026">
        <property type="entry name" value="fliI_yscN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00152">
        <property type="entry name" value="ATPASE_ALPHA_BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0066">ATP synthesis</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0139">CF(1)</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-0614">Plasmid</keyword>
    <keyword evidence="5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="AAA" evidence="9">
        <location>
            <begin position="201"/>
            <end position="384"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023310"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ABF12123.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="486" mass="51559" checksum="079D6CA863A11489" modified="2006-05-30" version="1">MHDEPAQATAPDAPAAHVGRWRDALAAASAGVSDIDSKRTCGRLTRAAGLVLEAVGLRLPVGSDCLIELPTGQHSHGGPRTAEAEVVGFGADRLYLMPQSDVAGLVPGARVYPLEPSPLPNGIIGAGAPREQGSKRLPVGDGLLGRVLDAAGRPLDGLGPITAASEVPLAGTQINPLTRAPIETVLDTGVRAINGMLTVGRGQRMGLFAGSGVGKSVLLGMMARYTNADVIVVGLIGERGREVKEFIENILGEEGRKRSVVVAAPADTSPLLRMQGAAYATRLAEYFRDKGQHVLLIMDSLTRYAMAQREIALAIGEPPATKGYPPSVFAKLPTLVERTGNGPEGGGSITAFYTVLTEGDDQQDPIADSARAILDGHIVLSRSLAEAGHYPAIDVEASISRAMTALIDAPQFASVRRFKQLMSRYQRNRDLISVGAYVPGSDPELDLAIRMYPRMEAFLQQDIHERAGYDASIAQLHDLFERTHHG</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LCN9</accession>
    <name>Q1LCN9_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Putative monooxygenase (Flavin-binding family)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Rmet_5228</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="6">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="6">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the FAD-binding monooxygenase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12087.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519633.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5228"/>
    <dbReference type="EnsemblBacteria" id="ABF12087">
        <property type="protein sequence ID" value="ABF12087"/>
        <property type="gene ID" value="Rmet_5228"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5228"/>
    <dbReference type="eggNOG" id="COG2072">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032067_2_0_4"/>
    <dbReference type="OMA" id="HTFGYEF"/>
    <dbReference type="OrthoDB" id="324612at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004499">
        <property type="term" value="F:N,N-dimethylaniline monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020946">
        <property type="entry name" value="Flavin_mOase-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00743">
        <property type="entry name" value="FMO-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="SSF51905"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0274">FAD</keyword>
    <keyword evidence="2" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="5" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-0614">Plasmid</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABF12087.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="509" mass="57342" checksum="B1E0284BBD53EECF" modified="2006-05-30" version="1">MNANYDVLIIGAGLSGIGMACHLAEACPGKRVGILERRSAIGGTWDLFRYPGIRSDSDMFSFGYKFRPWHELKVLADGPSIRRYIGDTAREYGVDKWIQFGLKVLRASWSSEDQYWTVTTQQEGSHDTRTLTCKFLIPCTGYYNYDHGHRPDFAGLERFQGICVHPQHWPEDLDYRGKRVVVIGSGATAVTLVPAMAGQASHVTMLQRSPSYVFSVPAYDKISAVLQKMLPARWVYGLARWRNIRLQRFIYRTAKRWPRRVRSWLLSHVEQQLGHGADMRHFSPSYNPWDQRLCAVPDGDLFKVISEGKASVVTDHIETFTETGVRLKSGKSLDADIIITATGLQLQSLGGMEVQVDGRPRHPSSLMTYKSVLMQDVPNLANLFGYTNASWTLKVDLAANYICRLLNHMDRHGYNVVTPIAPAGEMLEETVMDSLQSGYVQRGRSTLPRQGRTPPWRVLHNFVADREMLTRHPIHDPALRFVRAATTTTVARGRPACDLVATSMDETAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LCQ3</accession>
    <name>Q1LCQ3_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Glyoxalase/bleomycin resistance protein/dioxygenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_5214</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12073.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519618.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5214"/>
    <dbReference type="EnsemblBacteria" id="ABF12073">
        <property type="protein sequence ID" value="ABF12073"/>
        <property type="gene ID" value="Rmet_5214"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5214"/>
    <dbReference type="eggNOG" id="COG0346">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_153797_0_0_4"/>
    <dbReference type="OMA" id="FYEVKYR"/>
    <dbReference type="OrthoDB" id="1984437at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051213">
        <property type="term" value="F:dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004462">
        <property type="term" value="F:lactoylglutathione lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.180.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029068">
        <property type="entry name" value="Glyas_Bleomycin-R_OHBP_Dase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004360">
        <property type="entry name" value="Glyas_Fos-R_dOase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018146">
        <property type="entry name" value="Glyoxalase_1_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037523">
        <property type="entry name" value="VOC"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00903">
        <property type="entry name" value="Glyoxalase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54593">
        <property type="entry name" value="SSF54593"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00934">
        <property type="entry name" value="GLYOXALASE_I_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51819">
        <property type="entry name" value="VOC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="VOC" evidence="2">
        <location>
            <begin position="4"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="132"/>
            <end position="157"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF12073.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="157" mass="17066" checksum="AECA4854B0A471C8" modified="2006-05-30" version="1">MSSKLRHIALSVPDPWAAAEFYQQAFGFRKVGETDSSLARGVYLTDGTISIALLNYKSDHAAGEDRGKDFVGLHHIGVWVDDIVEARKTVEAAGGRYYMGEVPVKGNIFYEVKYRDPNGIIIDLTDHGWGGASKEANGGDEGPALRNPDLKADRSGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LCR0</accession>
    <name>Q1LCR0_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">2-keto-4-pentenoate hydratase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">mhpD</name>
        <name evidence="2" type="ordered locus">Rmet_5207</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12066.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519612.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5207"/>
    <dbReference type="EnsemblBacteria" id="ABF12066">
        <property type="protein sequence ID" value="ABF12066"/>
        <property type="gene ID" value="Rmet_5207"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5207"/>
    <dbReference type="eggNOG" id="COG3971">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_060136_4_0_4"/>
    <dbReference type="OMA" id="PLYEADM"/>
    <dbReference type="OrthoDB" id="1314120at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.850.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011234">
        <property type="entry name" value="Fumarylacetoacetase-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036663">
        <property type="entry name" value="Fumarylacetoacetase_C_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01557">
        <property type="entry name" value="FAA_hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56529">
        <property type="entry name" value="SSF56529"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAA_hydrolase" evidence="1">
        <location>
            <begin position="81"/>
            <end position="264"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF12066.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="273" mass="28490" checksum="95E98461D2F7B2D3" modified="2006-05-30" version="1">MLNPELIQVADQLRFASEHGQPCPPVRDAIARAASAGADPLALAYAVQQRNTEHALAAGRRLVGRKIGLTSVAVQRQLGVDQPDFGMLFADMVRQDGEEIDWGDVLQPKVETEIALVLEHDLPHAAHTVADVIRATAFALPAIEIVGSRIANWDIRLTDTIADNASSGLYVLGTRPVSLAALDLPHCGMVMELAGEPVSVGSGAACLGNPLNAAVWLANTMSRVGAPLRAGDTILTGALGPMVAVQPGNVFTASIEGLGSVTASFQAVRQGDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LCR5</accession>
    <name>Q1LCR5_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Dihydrodipicolinate synthetase</fullName>
            <ecNumber evidence="2">4.3.3.7</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_5202</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the DapA family.</text>
    </comment>
    <dbReference type="EC" id="4.3.3.7" evidence="2"/>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12061.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519607.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5202"/>
    <dbReference type="EnsemblBacteria" id="ABF12061">
        <property type="protein sequence ID" value="ABF12061"/>
        <property type="gene ID" value="Rmet_5202"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5202"/>
    <dbReference type="eggNOG" id="COG0329">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_049343_5_1_4"/>
    <dbReference type="OMA" id="VVPPVCT"/>
    <dbReference type="OrthoDB" id="1438588at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008840">
        <property type="term" value="F:4-hydroxy-tetrahydrodipicolinate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002220">
        <property type="entry name" value="DapA-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12128">
        <property type="entry name" value="PTHR12128"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00701">
        <property type="entry name" value="DHDPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001365">
        <property type="entry name" value="DHDPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00146">
        <property type="entry name" value="DHPICSNTHASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM01130">
        <property type="entry name" value="DHDPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 2" id="KW-0456">Lyase</keyword>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRNR" id="PIRNR001365"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF12061.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="300" mass="31842" checksum="54FB24D6AEB44AFD" modified="2006-05-30" version="1">MPTQQLRGVFTPVVTPFHEDLSINLPAFVAHCKRLIADGAGIAISGTNSEACSETFEERIALVDAAIDAGIPGERVLVGTGCCAIDDAVVLTRHAIERGCAGVLVLPPFFFKNVSEDGVFAYYSELIARVNDPALRIVLYHIPAMSGVPISLSLIERLVQAFPTHIVGIKDSSGNWDNLVAMLERFPSLSIFPASELLMLRSLSLGAAGCISATNNINARAIAQAFADAADAARLQPAIAAVRSTVERFPMVAAVKHTLAQQTSDPSWRHVRPPLVPLAADASTELLQALAGIPAMEARA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="72" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LCT1</accession>
    <name>Q1LCT1_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative Voltage-gated potassium channel</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_5186</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12045.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519592.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF12045">
        <property type="protein sequence ID" value="ABF12045"/>
        <property type="gene ID" value="Rmet_5186"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5186"/>
    <dbReference type="eggNOG" id="ENOG5032Y28">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_089632_1_0_4"/>
    <dbReference type="OMA" id="LVMLEQF"/>
    <dbReference type="OrthoDB" id="1991946at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013099">
        <property type="entry name" value="K_chnl_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07885">
        <property type="entry name" value="Ion_trans_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0407">Ion channel</keyword>
    <keyword evidence="3" id="KW-0406">Ion transport</keyword>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="43"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="65"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="94"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="126"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="170"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="201"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="domain" description="Ion_trans_2" evidence="2">
        <location>
            <begin position="144"/>
            <end position="215"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF12045.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="229" mass="25567" checksum="1A84DEA1E404D2E7" modified="2006-05-30" version="1">MAIRTTRDALSRIFYQRCFWLFVVLLTLIGAVSFVPPTDSGRLVLNIVNMFLLLATVAAVGRTTLSFVIVLLLAVPAMWFQYLGLWHDDDSSLAISWLFSVALYFITVAYLLRYVFQPRIMTPDKLFGAAAAYLLIGVLWAYVYASIGFFYPQSYMVVGQPGRLVYADALYFSITVLTSTGFGDITPLTRPARGMCMVEQITGSLFVAILIARLAGVYPPRESYTDDKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-10-07" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LCT8</accession>
    <name>Q1LCT8_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_5179</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12038.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF12038">
        <property type="protein sequence ID" value="ABF12038"/>
        <property type="gene ID" value="Rmet_5179"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5179"/>
    <dbReference type="HOGENOM" id="CLU_1729875_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF12038.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="151" mass="16797" checksum="E3CE51669F8F696E" modified="2006-05-30" version="1">MATTYRRHIINCNPQLLASGEYNPRLIVTEHQADAAIDHVIDLGPDVYRTAQEASQKAERAGMRWVDARVGPVHRHGDFGYEVSAASKERAQWTGEVRIYGSSSPANIFDRRTDYLVAPGIFRSDGEAMVVARAYAIQIIDAGTMFTWADV</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LCX3</accession>
    <name>Q1LCX3_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative acyl-CoA dehydrogenase</fullName>
            <ecNumber evidence="3">1.3.99.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_5144</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EC" id="1.3.99.-" evidence="3"/>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12003.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519550.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5144"/>
    <dbReference type="EnsemblBacteria" id="ABF12003">
        <property type="protein sequence ID" value="ABF12003"/>
        <property type="gene ID" value="Rmet_5144"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5144"/>
    <dbReference type="eggNOG" id="COG1960">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018204_9_1_4"/>
    <dbReference type="OMA" id="NAQHCQY"/>
    <dbReference type="OrthoDB" id="760677at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003995">
        <property type="term" value="F:acyl-CoA dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.540.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.110.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006089">
        <property type="entry name" value="Acyl-CoA_DH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006091">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046373">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013786">
        <property type="entry name" value="AcylCoA_DH/ox_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037069">
        <property type="entry name" value="AcylCoA_DH/ox_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009100">
        <property type="entry name" value="AcylCoA_DH/oxidase_NM_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02770">
        <property type="entry name" value="Acyl-CoA_dh_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02771">
        <property type="entry name" value="Acyl-CoA_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56645">
        <property type="entry name" value="SSF56645"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00072">
        <property type="entry name" value="ACYL_COA_DH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Acyl-CoA_dh_N" evidence="2">
        <location>
            <begin position="15"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA_dh_M" evidence="1">
        <location>
            <begin position="131"/>
            <end position="226"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF12003.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="385" mass="41949" checksum="876B201857A193C2" modified="2006-05-30" version="1">MNHLLDTFHLAQLPPEAEAFRAEVKAFLADMQPKAPPSKRARSWMGFDADFSRKLAGRGWVGITLPAQYGGAGLDPFRRFVLVEELLSAGAPVAAHWIADRQSGPLILRYGTEAQKAAYLPKICRGEAFFCIGMSEPNSGSDLASVRTRATPAADGWVLNGQKIWTTNAQHCQYMIALVRTSGVPEDKQKGLSQFIIDLSLPGVTVRPIRDLTGDAHFAEVVFENVALRSDALIGEEGSGWEQVNAELAFERSGPERIYSSIVLLDAWIEQLRRTEATRDQVAALGRFVTHVATLRNMSIAVTSKLARHESPVVEAALVKDLGTELEQAIPAYLEAAIASDPEAAGDGELLRTVAYLSQISPTFSLRGGTREILRGMIARSLGLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LCX4</accession>
    <name>Q1LCX4_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Putative acyl-CoA dehydrogenase</fullName>
            <ecNumber evidence="5">1.3.99.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Rmet_5143</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="6">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="6">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the acyl-CoA dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.3.99.-" evidence="5"/>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12002.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519549.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5143"/>
    <dbReference type="EnsemblBacteria" id="ABF12002">
        <property type="protein sequence ID" value="ABF12002"/>
        <property type="gene ID" value="Rmet_5143"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5143"/>
    <dbReference type="eggNOG" id="COG1960">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018204_5_0_4"/>
    <dbReference type="OMA" id="ITIAPRC"/>
    <dbReference type="OrthoDB" id="760677at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016627">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-CH group of donors"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.540.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036250">
        <property type="entry name" value="AcylCo_DH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009075">
        <property type="entry name" value="AcylCo_DH/oxidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013786">
        <property type="entry name" value="AcylCoA_DH/ox_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037069">
        <property type="entry name" value="AcylCoA_DH/ox_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009100">
        <property type="entry name" value="AcylCoA_DH/oxidase_NM_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00441">
        <property type="entry name" value="Acyl-CoA_dh_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02771">
        <property type="entry name" value="Acyl-CoA_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47203">
        <property type="entry name" value="SSF47203"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56645">
        <property type="entry name" value="SSF56645"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0274">FAD</keyword>
    <keyword evidence="2" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-0614">Plasmid</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Acyl-CoA_dh_N" evidence="4">
        <location>
            <begin position="1"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA_dh_1" evidence="3">
        <location>
            <begin position="181"/>
            <end position="291"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF02771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABF12002.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="327" mass="34447" checksum="50D0FF4B9394C7D6" modified="2006-05-30" version="1">MFVEAIANILKDRFSPQALRKIGQGVEVREVWGAIEEAGFLELLAPEEAGGAGLALGELYPILLEVGKHAVPLPIGQTIAARAILSKSGGGAPAGMITIAPRCRCEPDGSLHIANVIGGLVADHVLVGCGDAVLLLDCSAAQRDWIGVHGSLSADLKWVRGDAVPIEGVTVDELDAWDGAVLAVLMAGAARRAGELTLQYCQDRSQFGRSIGKFQVIQHQLSVIVEHIAAMSVAAQMAFAGGQRHPGKLAAAVAKARCAEAVQVVAAAAHGLHGAIGITEEYDLQLVTRCLHEWRMAHGSELYWNRVVGMAILGRDGVKVSDFARQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-10-07" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LD01</accession>
    <name>Q1LD01_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Fimbrial protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_5116</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11975.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5116"/>
    <dbReference type="EnsemblBacteria" id="ABF11975">
        <property type="protein sequence ID" value="ABF11975"/>
        <property type="gene ID" value="Rmet_5116"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5116"/>
    <dbReference type="eggNOG" id="COG3539">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_058392_5_0_4"/>
    <dbReference type="OMA" id="FMPHAKA"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009289">
        <property type="term" value="C:pilus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007155">
        <property type="term" value="P:cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1090">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000259">
        <property type="entry name" value="Adhesion_dom_fimbrial"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036937">
        <property type="entry name" value="Adhesion_dom_fimbrial_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008966">
        <property type="entry name" value="Adhesion_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00419">
        <property type="entry name" value="Fimbrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49401">
        <property type="entry name" value="SSF49401"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193073" evidence="1">
        <location>
            <begin position="29"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="domain" description="Fimbrial" evidence="2">
        <location>
            <begin position="191"/>
            <end position="339"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00419"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF11975.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="339" mass="34513" checksum="8465999587AA972D" modified="2006-05-30" version="1">MIRRVWNETLQKMLCGLALVAVSDAVHALGDCSTSPSMPYLQAMNNMAVAVNLAVGAAIPGTRRNYTFSGRCVADGNNLIYAGAPIIACYYGSGREITPGVYSTGVAGIGIRLRNAVGQPLTNAIGVTCDTRAAALGALAADLSYSVSVSIEFVKTGAIGQGSLDPSQTLFGFGVYRSAVGLGGGGNHIGFTGSATPRQITCTTRAPTTVALPDATVSSLSGNGSTTGFAPFSIGLICDGAAVVGISFDGAGGTPVRSASTGVLGIQNEGAAGMASGVGVQLINDGTYSPVPLQTRNHLGNISANGMASYRYAFRYHSLSARPSPGTVRGTMVFTFDYQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="61" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LD71</accession>
    <name>Q1LD71_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Phasin_2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_5043</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11905.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5043"/>
    <dbReference type="EnsemblBacteria" id="ABF11905">
        <property type="protein sequence ID" value="ABF11905"/>
        <property type="gene ID" value="Rmet_5043"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5043"/>
    <dbReference type="eggNOG" id="COG5490">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1266052_0_0_4"/>
    <dbReference type="OMA" id="QFKLPGF"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018968">
        <property type="entry name" value="Phasin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010127">
        <property type="entry name" value="Phasin_subfam-1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09361">
        <property type="entry name" value="Phasin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01841">
        <property type="entry name" value="phasin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Phasin_2" evidence="2">
        <location>
            <begin position="105"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF09361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF11905.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="218" mass="23907" checksum="E1A434CB63E09F0D" modified="2006-05-30" version="1">MQRDRAGQSGRLPGPDDTRTRVPCQPLGAPRRYPPYSFPSSLAARITLSLPRGDRATSPVSRRRSTSACSTPTRRFVMATAPNPFTDITKLLEQYKLPGVDMTSIIEARRKDIEALAEANRIAYEGMQALVQKQTEILSKSMQEIQATAQKMATSGNPAEAMTRQGELVQQGLQTAFNNMRELAEMAQKSQAEALAVITKRAEQSIAEAKSLMKPAGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LDD8</accession>
    <name>Q1LDD8_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">EamA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_3006</name>
        <name evidence="4" type="ordered locus">Rmet_4041</name>
        <name evidence="5" type="ordered locus">Rmet_4976</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="5">
        <name>megaplasmid</name>
    </geneLocation>
    <geneLocation type="plasmid" evidence="6">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="5" key="1">
        <citation type="submission" date="2006-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of the chromosome of Ralstonia metallidurans CH34.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Copeland A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Barry K."/>
                <person name="Detter J.C."/>
                <person name="Glavina del Rio T."/>
                <person name="Hammon N."/>
                <person name="Israni S."/>
                <person name="Dalin E."/>
                <person name="Tice H."/>
                <person name="Martinez M."/>
                <person name="Goltsman E."/>
                <person name="Pitluck S."/>
                <person name="Schmutz J."/>
                <person name="Larimer F."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Kim E."/>
                <person name="Mergeay M."/>
                <person name="Benotmane M.A."/>
                <person name="Vallaeys T."/>
                <person name="Michaux A."/>
                <person name="Monchy S."/>
                <person name="Dunn J."/>
                <person name="McCorkle S."/>
                <person name="Taghavi S."/>
                <person name="van der Lelie D."/>
                <person name="Richardson P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">CH34</strain>
            <plasmid evidence="5">megaplasmid</plasmid>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="6">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2010-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Benotmane R."/>
                <person name="Lapidus A."/>
                <person name="McCorkle S."/>
                <person name="Monchy S."/>
                <person name="Taghavi S."/>
                <person name="van der Lelie N."/>
                <person name="Dunn J."/>
                <person name="Leys N."/>
                <person name="Mergeay M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">CH34</strain>
            <plasmid evidence="5">megaplasmid</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09879.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10909.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11838.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_005797960.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3006"/>
    <dbReference type="EnsemblBacteria" id="ABF09879">
        <property type="protein sequence ID" value="ABF09879"/>
        <property type="gene ID" value="Rmet_3006"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF10909">
        <property type="protein sequence ID" value="ABF10909"/>
        <property type="gene ID" value="Rmet_4041"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF11838">
        <property type="protein sequence ID" value="ABF11838"/>
        <property type="gene ID" value="Rmet_4976"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3006"/>
    <dbReference type="KEGG" id="rme:Rmet_4041"/>
    <dbReference type="KEGG" id="rme:Rmet_4976"/>
    <dbReference type="eggNOG" id="COG2510">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_120467_0_0_4"/>
    <dbReference type="OMA" id="VNSDFAT"/>
    <dbReference type="OrthoDB" id="1668457at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000620">
        <property type="entry name" value="EamA_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00892">
        <property type="entry name" value="EamA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0614">Plasmid</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="39"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="69"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="124"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="domain" description="EamA" evidence="2">
        <location>
            <begin position="8"/>
            <end position="141"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF09879.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF10909.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABF11838.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="143" mass="15255" checksum="1B8C77398B1809FF" modified="2006-05-30" version="1">MVNSAGWFYWALLSAILAALTAIFAKVGIQGVDSDLATLIRTAIIIVVLSAFVAYTGKWTNPLELSPKTWLFLGLSGLATGASWVCYFRALKIGDASKVAPVDKLSLVLVAVFAFAFLGERPSLREWSGISMVAGGVLLLAFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="107" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LDD9</accession>
    <name>Q1LDD9_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Serine-based site-specific recombinase activity (Resolvase) (Tn6048)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">tniR</name>
        <name evidence="7" type="ordered locus">Rmet_3007</name>
        <name evidence="8" type="ordered locus">Rmet_4042</name>
        <name evidence="9" type="ordered locus">Rmet_4975</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="9">
        <name>megaplasmid</name>
    </geneLocation>
    <geneLocation type="plasmid" evidence="10">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="9" key="1">
        <citation type="submission" date="2006-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of the chromosome of Ralstonia metallidurans CH34.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Copeland A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Barry K."/>
                <person name="Detter J.C."/>
                <person name="Glavina del Rio T."/>
                <person name="Hammon N."/>
                <person name="Israni S."/>
                <person name="Dalin E."/>
                <person name="Tice H."/>
                <person name="Martinez M."/>
                <person name="Goltsman E."/>
                <person name="Pitluck S."/>
                <person name="Schmutz J."/>
                <person name="Larimer F."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Kim E."/>
                <person name="Mergeay M."/>
                <person name="Benotmane M.A."/>
                <person name="Vallaeys T."/>
                <person name="Michaux A."/>
                <person name="Monchy S."/>
                <person name="Dunn J."/>
                <person name="McCorkle S."/>
                <person name="Taghavi S."/>
                <person name="van der Lelie D."/>
                <person name="Richardson P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">CH34</strain>
            <plasmid evidence="9">megaplasmid</plasmid>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="10">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2010-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Benotmane R."/>
                <person name="Lapidus A."/>
                <person name="McCorkle S."/>
                <person name="Monchy S."/>
                <person name="Taghavi S."/>
                <person name="van der Lelie N."/>
                <person name="Dunn J."/>
                <person name="Leys N."/>
                <person name="Mergeay M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">CH34</strain>
            <plasmid evidence="9">megaplasmid</plasmid>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the site-specific recombinase resolvase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09880.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10910.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11837.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517527.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3007"/>
    <dbReference type="EnsemblBacteria" id="ABF09880">
        <property type="protein sequence ID" value="ABF09880"/>
        <property type="gene ID" value="Rmet_3007"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF10910">
        <property type="protein sequence ID" value="ABF10910"/>
        <property type="gene ID" value="Rmet_4042"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF11837">
        <property type="protein sequence ID" value="ABF11837"/>
        <property type="gene ID" value="Rmet_4975"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3007"/>
    <dbReference type="KEGG" id="rme:Rmet_4042"/>
    <dbReference type="KEGG" id="rme:Rmet_4975"/>
    <dbReference type="eggNOG" id="COG1961">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010686_8_0_4"/>
    <dbReference type="OMA" id="YRHISPK"/>
    <dbReference type="OrthoDB" id="1791095at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000150">
        <property type="term" value="F:DNA strand exchange activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1390">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006118">
        <property type="entry name" value="Recombinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006119">
        <property type="entry name" value="Resolv_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036162">
        <property type="entry name" value="Resolvase-like_N_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00239">
        <property type="entry name" value="Resolvase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00857">
        <property type="entry name" value="Resolvase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53041">
        <property type="entry name" value="SSF53041"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00398">
        <property type="entry name" value="RECOMBINASES_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51736">
        <property type="entry name" value="RECOMBINASES_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0229">DNA integration</keyword>
    <keyword evidence="4" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="9" id="KW-0614">Plasmid</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Resolvase/invertase-type recombinase catalytic" evidence="6">
        <location>
            <begin position="1"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="active site" description="O-(5'-phospho-DNA)-serine intermediate" evidence="5">
        <location>
            <position position="9"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR606118-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51736"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABF09880.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ABF10910.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ABF11837.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="204" mass="22112" checksum="7A7221CDA100183B" modified="2006-05-30" version="1">MLIGYMRVSKADGSQATDLQRDALIAAGVDPAHLYEDQASGKREDRPGLLSCLKALRPGDTLMVWKLDRLGRDLRHLINTVHDLTGRGIGLKVLTGHGAAIDTTTAAGKLVFGIFAALAEFERELITERTIAGLASARARGRKGGRPFKMTAAKLRLAMAAMGQPETKVGDLCQELGITRQTLYRHISPKGELRLDGEKLLSQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="102" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LDU3</accession>
    <name>Q1LDU3_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Assimilatory nitrate reductase (NADH) large subunit</fullName>
            <ecNumber evidence="11">1.7.99.4</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">nasA</name>
        <name evidence="11" type="ordered locus">Rmet_4821</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="12">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="12">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mo-bis(molybdopterin guanine dinucleotide)</name>
            <dbReference type="ChEBI" id="CHEBI:60539"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/NapA/NarB subfamily.</text>
    </comment>
    <dbReference type="EC" id="1.7.99.4" evidence="11"/>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11683.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519246.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4821"/>
    <dbReference type="EnsemblBacteria" id="ABF11683">
        <property type="protein sequence ID" value="ABF11683"/>
        <property type="gene ID" value="Rmet_4821"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4821"/>
    <dbReference type="eggNOG" id="COG0243">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000422_13_4_4"/>
    <dbReference type="OMA" id="NLAWCHP"/>
    <dbReference type="OrthoDB" id="323168at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043546">
        <property type="term" value="F:molybdopterin cofactor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008940">
        <property type="term" value="F:nitrate reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042128">
        <property type="term" value="P:nitrate assimilation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02791">
        <property type="entry name" value="MopB_CT_Nitrate-R-NapA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.1100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009010">
        <property type="entry name" value="Asp_de-COase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007419">
        <property type="entry name" value="BFD-like_2Fe2S-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041854">
        <property type="entry name" value="BFD-like_2Fe2S-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041957">
        <property type="entry name" value="CT_Nitrate-R-NapA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006657">
        <property type="entry name" value="MoPterin_dinucl-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006656">
        <property type="entry name" value="Mopterin_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006963">
        <property type="entry name" value="Mopterin_OxRdtase_4Fe-4S_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027467">
        <property type="entry name" value="MopterinOxRdtase_cofactor_BS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04324">
        <property type="entry name" value="Fer2_BFD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04879">
        <property type="entry name" value="Molybdop_Fe4S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00384">
        <property type="entry name" value="Molybdopterin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01568">
        <property type="entry name" value="Molydop_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00926">
        <property type="entry name" value="Molybdop_Fe4S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50692">
        <property type="entry name" value="SSF50692"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51669">
        <property type="entry name" value="4FE4S_MOW_BIS_MGD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00551">
        <property type="entry name" value="MOLYBDOPTERIN_PROK_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="6 9" id="KW-0408">Iron</keyword>
    <keyword evidence="7 9" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="5 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0500">Molybdenum</keyword>
    <keyword evidence="8" id="KW-0534">Nitrate assimilation</keyword>
    <keyword evidence="11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-0614">Plasmid</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="4Fe-4S Mo/W bis-MGD-type" evidence="10">
        <location>
            <begin position="14"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="21"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="24"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="28"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="56"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022505"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51669"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ABF11683.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="915" mass="99231" checksum="25A4DB916B0C30BE" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LDV1</accession>
    <name>Q1LDV1_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative short-chain dehydrogenase/reductase SDR</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_4813</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11675.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519238.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4813"/>
    <dbReference type="EnsemblBacteria" id="ABF11675">
        <property type="protein sequence ID" value="ABF11675"/>
        <property type="gene ID" value="Rmet_4813"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4813"/>
    <dbReference type="eggNOG" id="COG4221">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010194_1_0_4"/>
    <dbReference type="OMA" id="DWWVASI"/>
    <dbReference type="OrthoDB" id="1356861at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00061">
        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU000363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF11675.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="239" mass="24851" checksum="267A967662F6CCFE" modified="2006-05-30" version="1">MDSTAARRHVVITGAAGALGRAVASRFAAEGARLALIDRDLQHLQSVFAHPEPDHGGTLLHAADVTSDTAMAPVAAAILDAFGTVDVLVHVAGGFEMGEATHAMSRESWMRMMDLNAWSFVAVTSHFIPAMLFQRHGKVVAVSARGAMAGAATMAAYAASKSALQRLVESLSHEVRGAGINVNSVAPSILDTPANRQAMPSVDHTRWVSTGAAARAVAFLASDAAEAVHGQHMVLDGLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="67" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LDW9</accession>
    <name>Q1LDW9_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative general (Type II) secretion pathway protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_4795</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11657.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4795"/>
    <dbReference type="EnsemblBacteria" id="ABF11657">
        <property type="protein sequence ID" value="ABF11657"/>
        <property type="gene ID" value="Rmet_4795"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4795"/>
    <dbReference type="eggNOG" id="COG2165">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1239312_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045584">
        <property type="entry name" value="Pilin-like"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54523">
        <property type="entry name" value="SSF54523"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="94"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="45"/>
            <end position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF11657.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="223" mass="24520" checksum="3B0CBA27661ED487" modified="2006-05-30" version="1">MQMISLRTSSCVSRDSQCCAEIWRWDAIVASAPLAAPQKHLKTSVGSNVTGTSADRRYPPQLHRNTVPNQTHMACPQGLTMTISMATARRRARFTLATLLAALIGIGLIAQIALIAPHLTAEAEPSQEVALRNNLLAMREAITRFHVDHGHYPQSLDTLVQRAYLHRIPQDPITHTADNWEIVPPPIDPQSPTMLEDRAIAGVFDVRSGALGVTHEGTPYREL</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-10-07" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LEB9</accession>
    <name>Q1LEB9_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Extra-cytoplasmic solute receptor protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">bug</name>
        <name evidence="4" type="ordered locus">Rmet_4645</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="5">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="5">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Periplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the UPF0065 (bug) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11507.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4645"/>
    <dbReference type="EnsemblBacteria" id="ABF11507">
        <property type="protein sequence ID" value="ABF11507"/>
        <property type="gene ID" value="Rmet_4645"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4645"/>
    <dbReference type="eggNOG" id="COG3181">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045683_0_1_4"/>
    <dbReference type="OMA" id="TNTTHAM"/>
    <dbReference type="OrthoDB" id="447734at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042597">
        <property type="term" value="C:periplasmic space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005064">
        <property type="entry name" value="BUG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042100">
        <property type="entry name" value="Bug_dom1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42928">
        <property type="entry name" value="PTHR42928"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03401">
        <property type="entry name" value="TctC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017082">
        <property type="entry name" value="YflP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-0675">Receptor</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193409" evidence="3">
        <location>
            <begin position="39"/>
            <end position="348"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF11507.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="348" mass="36549" checksum="0FAE76AD2BE4F10A" modified="2006-05-30" version="1">MDASLVHARPRRLRPLRPLRPLPALLPTALSIATVCLAAVTPVPGQAASDYPSRPIKLVLGFPPGGSTDVGARRVADQLGKRLGQPVVVENRPGASGNIAAAYVAKTPPDGYTLFYGTNTTHAMNVSLYPKLGYDPVKDFVPIVLMGKVWNVLSVNPSYKATSLAELIAMAKAAPGQIDVATPGNSTSPHMSLVLLESRAGIKITHVPYKGSAAALNDVMGGQTSVLFDNLPASLPYIKAGKLRALAVSSPKRLSVLPDVPTFDELGIKGYEVAGWGALWAPAGTPQAIVDRLNRETNAVLKDPTMIAQMEALASDPQGGTAKSLAAFAATETQRWGEVIRAAGLKLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="59" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LEC1</accession>
    <name>Q1LEC1_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_4643</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11505.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4643"/>
    <dbReference type="EnsemblBacteria" id="ABF11505">
        <property type="protein sequence ID" value="ABF11505"/>
        <property type="gene ID" value="Rmet_4643"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4643"/>
    <dbReference type="HOGENOM" id="CLU_131512_0_0_4"/>
    <dbReference type="OMA" id="KCLRNNL"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF11505.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="174" mass="18878" checksum="0DEAA4A8AC7701D2" modified="2006-05-30" version="1">MCQGNEVCTMPAPWLARRRKHARRPARLRTTHARGAGVSGKHMQKTRVLLIAALAAPLLAACAGGGNTPDYIQSQEPKRIHSNASVGDLVQCLKSKLQEEATIVPYAEPGKVDIRIGHASASEMRYFYLIYLRAAAQGSDVEIRSAGEWHPILRQGRIEGIVEDCKPGAASARG</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LEE5</accession>
    <name>Q1LEE5_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">RND efflux system, outer membrane lipoprotein, NodT</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">nodT</name>
        <name evidence="3" type="ordered locus">Rmet_4619</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the outer membrane factor (OMF) (TC 1.B.17) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11481.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519056.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4619"/>
    <dbReference type="EnsemblBacteria" id="ABF11481">
        <property type="protein sequence ID" value="ABF11481"/>
        <property type="gene ID" value="Rmet_4619"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4619"/>
    <dbReference type="eggNOG" id="COG1538">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012817_13_1_4"/>
    <dbReference type="OMA" id="AGSIWNV"/>
    <dbReference type="OrthoDB" id="1619477at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015562">
        <property type="term" value="F:efflux transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003423">
        <property type="entry name" value="OMP_efflux"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010131">
        <property type="entry name" value="RND_efflux_OM_lipoprot_NodT"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02321">
        <property type="entry name" value="OEP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01845">
        <property type="entry name" value="outer_NodT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0564">Palmitate</keyword>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1134">Transmembrane beta strand</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5001442048" evidence="2">
        <location>
            <begin position="26"/>
            <end position="476"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU362097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF11481.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="476" mass="50968" checksum="766BC7520662E653" modified="2006-05-30" version="1">MSSSQIKKSLLLALPVAMLAGCALQTPPKGEDLRQQTVPSLEGRAGWANPTSGDAVADGWLAAFDDAELQRLVREAIAHNGDLRLAEARVQQAQALVAIARSGLLPSAGVKGRAGNSETQILSIGASWEVDLWGRIRAESRSAESQYAATQDDYLWAQRVIAATTAKAWFSLIRYTVLEDRLRQSATIQEELVRIVGQRVTIGIAPETDLLEARNTLHAQRDGIEKTALARSQAAQALELLLGRYPAGDIATAPTLPAMPPAPNASLPANVVERRPDLTAAAHRVAAAFDMRQSAEAARLPRISISAAVTGIRSDVFLLNQAGSPVKGLNGAFFAPIFMGGELKARVDYYTAEQKAALIAYGNSALRALGEVETGLRAESGYAERTRQLRERVNERRVLVQREETRVTIGASDTRSLQQQRQTLVAAEMDLVNVEADHLDQRVALLLALGGDWRPETTATATATATATGTGTGTGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LEZ7</accession>
    <name>Q1LEZ7_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">3-oxoacyl-(Acyl-carrier-protein) reductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">fabG</name>
        <name evidence="1" type="ordered locus">Rmet_4414</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11279.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518893.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4414"/>
    <dbReference type="EnsemblBacteria" id="ABF11279">
        <property type="protein sequence ID" value="ABF11279"/>
        <property type="gene ID" value="Rmet_4414"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4414"/>
    <dbReference type="eggNOG" id="COG1028">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010194_1_3_4"/>
    <dbReference type="OMA" id="HAAAYWK"/>
    <dbReference type="OrthoDB" id="1601931at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00061">
        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF11279.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="304" mass="32288" checksum="F2E4CB9BD71A69B2" modified="2006-05-30" version="1">MQGIMEGKVALVTGAGGGIGRGIALALGAAGARVVVNDLGVSMSGEGGDAGPAQRVAEEIRAAGGQAVANTDSVSTWNGAHNIIQCALDNFGRIDAVVNNAGNLRDRMFFKMNEEEWRSVIDVHLHGTFFVSRAAANHFKDQEGGAYVHMTSTSGLIGNFGQANYAAAKLGIVALSKSIALDMQRFNVRSNCIAPFAWSRMTSSIPAETPEEKARVAKLQRMEAGKIAPMAVFLASDAARDVSGQIFAVRANEIMLMSHSRPIRSVHMGEGWTPEAIGEVAVPAMRNSFYKLERSADVIGWDPI</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-10-07" version="67" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LF23</accession>
    <name>Q1LF23_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative Phospho-N-acetylmuramoyl-pentapeptide-transferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_4388</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11253.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4388"/>
    <dbReference type="EnsemblBacteria" id="ABF11253">
        <property type="protein sequence ID" value="ABF11253"/>
        <property type="gene ID" value="Rmet_4388"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4388"/>
    <dbReference type="eggNOG" id="ENOG50330BN">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_120407_1_0_4"/>
    <dbReference type="OMA" id="LFNLMIT"/>
    <dbReference type="OrthoDB" id="2073232at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021675">
        <property type="entry name" value="DUF3261"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11659">
        <property type="entry name" value="DUF3261"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193439" evidence="1">
        <location>
            <begin position="21"/>
            <end position="190"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF11253.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="190" mass="20765" checksum="52728EE65CBFF943" modified="2006-05-30" version="1">MRLPAALATLAALAALAVLAGCATVAPTRAEVPLLRLPPASLGRTLSLQQQITVQFPSPQGEQTRDMIAMLEADAAHTRLAAVAGGQVLARIDWDGRDLRVTRAPWAPRELMPERILSDLQLSLWPVEAIQAALPDGWHVDATPAMRVLRYGDEVVAEVRYPDATTTLFTQRRDGYRLTIRTLQEEGKTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-10-07" version="69" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LFC4</accession>
    <name>Q1LFC4_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Extra-cytoplasmatic solute receptor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">bug</name>
        <name evidence="3" type="ordered locus">Rmet_4286</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Periplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the UPF0065 (bug) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11152.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4286"/>
    <dbReference type="EnsemblBacteria" id="ABF11152">
        <property type="protein sequence ID" value="ABF11152"/>
        <property type="gene ID" value="Rmet_4286"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4286"/>
    <dbReference type="eggNOG" id="COG3181">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045683_0_0_4"/>
    <dbReference type="OMA" id="MAESIPQ"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042597">
        <property type="term" value="C:periplasmic space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005064">
        <property type="entry name" value="BUG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042100">
        <property type="entry name" value="Bug_dom1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42928">
        <property type="entry name" value="PTHR42928"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03401">
        <property type="entry name" value="TctC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017082">
        <property type="entry name" value="YflP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-0675">Receptor</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF11152.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="353" mass="37091" checksum="69071BB9C134F31A" modified="2006-05-30" version="1">MSYKRHNTGPIHASQSVAVSGERKMTMPVARRVLHALATLALTPLAFAAGAAHGAWPEKPIRMVVGFPPGGPVDTHARILAEKLQPILGQSVVLDYKAGAAGNIGSDNVAKSAPDGYTLLIANTGQMTINNAIYPKLPYSMPKDFAPVARTALIPLVMVVNNNVPAKNLKEFIAYAKANPGKLNFASGGNGGISHLMPEMFKQASGTFIVHIPYKGSAPALTDVMGGQAEMMADSIPLFTQYIKGGKVRALAVTSGKRSPALPDVPTMEEAGLKGFEVVGFYGVLAPAGTPKDIVDKLSAALRTVLADPDTKAKLEFQGAEPAWQSPDAFAATIASEQKRWGQAVKASGARID</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LFD1</accession>
    <name>Q1LFD1_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative fusaric acid resistance protein, conserved region</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_4279</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11145.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518771.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4279"/>
    <dbReference type="EnsemblBacteria" id="ABF11145">
        <property type="protein sequence ID" value="ABF11145"/>
        <property type="gene ID" value="Rmet_4279"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4279"/>
    <dbReference type="eggNOG" id="COG1289">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013927_0_0_4"/>
    <dbReference type="OMA" id="MITQACE"/>
    <dbReference type="OrthoDB" id="1320135at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006726">
        <property type="entry name" value="PHBA_efflux_AaeB/fusaric-R"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04632">
        <property type="entry name" value="FUSC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="79"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="105"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="131"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="157"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="397"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="453"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="477"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="535"/>
            <end position="558"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF11145.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="728" mass="77977" checksum="9B363F3CBC9A5159" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="109" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LFF6</accession>
    <name>Q1LFF6_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Histidine kinase</fullName>
            <ecNumber evidence="2">2.7.13.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">Rmet_4254</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="11">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="11">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.</text>
            <dbReference type="EC" id="2.7.13.3"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.7.13.3" evidence="2"/>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11120.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518746.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4254"/>
    <dbReference type="EnsemblBacteria" id="ABF11120">
        <property type="protein sequence ID" value="ABF11120"/>
        <property type="gene ID" value="Rmet_4254"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4254"/>
    <dbReference type="eggNOG" id="COG2199">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG4251">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_403790_0_0_4"/>
    <dbReference type="OMA" id="NSERREH"/>
    <dbReference type="OrthoDB" id="1755994at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
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        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="HAMP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003661">
        <property type="entry name" value="HisK_dim/P"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036097">
        <property type="entry name" value="HisK_dim/P_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004358">
        <property type="entry name" value="Sig_transdc_His_kin-like_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00672">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00512">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00344">
        <property type="entry name" value="BCTRLSENSOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00304">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00388">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47384">
        <property type="entry name" value="SSF47384"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="SSF55874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50885">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 10" id="KW-0418">Kinase</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="10" id="KW-0614">Plasmid</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="315"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="domain" description="HAMP" evidence="9">
        <location>
            <begin position="338"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="8">
        <location>
            <begin position="437"/>
            <end position="658"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="658"/>
            <end position="681"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="667"/>
            <end position="681"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ABF11120.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="681" mass="73252" checksum="D21CA1B52505B4E8" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LFX0</accession>
    <name>Q1LFX0_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Paraquat-inducible protein A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">pqiA3</name>
        <name evidence="8" type="ordered locus">Rmet_4089</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="9">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="9">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PqiA family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10956.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518583.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4089"/>
    <dbReference type="EnsemblBacteria" id="ABF10956">
        <property type="protein sequence ID" value="ABF10956"/>
        <property type="gene ID" value="Rmet_4089"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4089"/>
    <dbReference type="eggNOG" id="COG2995">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041903_0_1_4"/>
    <dbReference type="OMA" id="WVMFDVY"/>
    <dbReference type="OrthoDB" id="836803at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007498">
        <property type="entry name" value="PqiA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005219">
        <property type="entry name" value="PqiA-like_Ent"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04403">
        <property type="entry name" value="PqiA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00155">
        <property type="entry name" value="pqiA_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0614">Plasmid</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="47"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="95"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="142"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="170"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="267"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="312"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="360"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="387"/>
            <end position="407"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ABF10956.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="423" mass="45631" checksum="D60A19ED8974CBB1" modified="2006-05-30" version="1">MTRTDLIACPECDLLQRALPVPIKGTLRCCRCDAELARNHPDGLEQALALALGALVLLGISNLYPIVGLSVSGTLVQTTLAGAVRVLFHGGMWPLAILVLITTIVMPVLQTVGMLWVLLPLRLGRKPWFAPQVLRLCHQAESWGMTEVLILGLLVALVKLAHIASVVPGVALWSFGALMLVLVGAHTAFDPHSLWVRFSDVRTTSGARPHLEPPIVSGPITAAHCGLLSCHDCGLLTSAHTRDHALACPRCGATLHSRKPASLTRTWALLISAVVLYIPANVLPVMYTSSLFGAENDTILSGVAYLWTSGSWLLAIVVFIASIAVPMLKILALGYLAFSTQFHSTSAPKRRTKIYRVVEFVGRWSMLDIYVITVLVALVQFKALATIQAGPAAIAFGAVVVLTMFAAMTFDPRLIWDEAEHQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-10-07" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LFX6</accession>
    <name>Q1LFX6_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_4083</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10950.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4083"/>
    <dbReference type="EnsemblBacteria" id="ABF10950">
        <property type="protein sequence ID" value="ABF10950"/>
        <property type="gene ID" value="Rmet_4083"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4083"/>
    <dbReference type="HOGENOM" id="CLU_2384048_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF10950.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="94" mass="10688" checksum="3C3EFAEBB97669A5" modified="2006-05-30" version="1">MAAMLQRIRYMAMATGRVAARKALHFCNTSRHTTGSHSPKWARNDYEPHPVAGGSQFACFRHCSGCFAEFPGERRHRHRLRVNDEAPRELAPNP</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="67" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LGI0</accession>
    <name>Q1LGI0_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative sterol desaturase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_3878</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10746.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518393.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3878"/>
    <dbReference type="EnsemblBacteria" id="ABF10746">
        <property type="protein sequence ID" value="ABF10746"/>
        <property type="gene ID" value="Rmet_3878"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3878"/>
    <dbReference type="eggNOG" id="COG3000">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033631_1_1_4"/>
    <dbReference type="OMA" id="RFWWATH"/>
    <dbReference type="OrthoDB" id="2048011at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008610">
        <property type="term" value="P:lipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006694">
        <property type="entry name" value="Fatty_acid_hydroxylase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04116">
        <property type="entry name" value="FA_hydroxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="54"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="83"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="domain" description="Fatty acid hydroxylase" evidence="2">
        <location>
            <begin position="87"/>
            <end position="220"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF04116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF10746.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="307" mass="35183" checksum="F035C3AFC73FDE20" modified="2006-05-30" version="1">MVITGKFAMYVTAFVVTVSLIEAFVLWRKRRDGVKPFSWNEVWLSLADLAGRKLSVLLPLSLAAPVFSFAWDHRLFTITLNSALAVFLLFVGQEFCYYWYHRAAHRIRFFWATHAVHHSPNQLTLTSAYRLGWTGKLTGSAVFFAPLVLLGVRPEVVLATLSLNLLYQFWLHNDWMPKLGWFEYVFNTPSAHRVHHASNIDYLDANYGGVLIIFDRLFGTYVEERADEPCRYGLVHPTTTQNPIVNQFEHWVSLGRDMASASKLWHAAAYLIMPPGWAPNGKGTTTEILRRQQEELRQAGWQRPGVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="97" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LGI5</accession>
    <name>Q1LGI5_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_3873</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10741.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518388.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3873"/>
    <dbReference type="EnsemblBacteria" id="ABF10741">
        <property type="protein sequence ID" value="ABF10741"/>
        <property type="gene ID" value="Rmet_3873"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3873"/>
    <dbReference type="eggNOG" id="COG1846">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_083287_8_0_4"/>
    <dbReference type="OMA" id="WTDLDEA"/>
    <dbReference type="OrthoDB" id="1744037at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00090">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011991">
        <property type="entry name" value="ArsR-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000835">
        <property type="entry name" value="HTH_MarR-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12802">
        <property type="entry name" value="MarR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00347">
        <property type="entry name" value="HTH_MARR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50995">
        <property type="entry name" value="HTH_MARR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH marR-type" evidence="2">
        <location>
            <begin position="31"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF10741.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="172" mass="18952" checksum="50ADC2ED97BD3F09" modified="2006-05-30" version="1">MSSTTRSASVDTGTTDPWSNLDEAGTDLNVNDFLTTMLSQLVTALRSNVTEPYASRFELTVPEWRILALLAHGETMSFAELVRQSTSDKALVSRTLRLLEDRGLVTLESEGGTRRRLTCRITPEGAALHAQVIPLARRGQASVIRMLSPEERRGLYSGLRKLHGLCTTPNKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="92" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LGJ0</accession>
    <name>Q1LGJ0_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative cytochrome P450</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_3868</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the cytochrome P450 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10736.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518383.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3868"/>
    <dbReference type="EnsemblBacteria" id="ABF10736">
        <property type="protein sequence ID" value="ABF10736"/>
        <property type="gene ID" value="Rmet_3868"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3868"/>
    <dbReference type="eggNOG" id="COG2124">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001570_5_1_4"/>
    <dbReference type="OMA" id="IFRRMKM"/>
    <dbReference type="OrthoDB" id="520970at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="SSF48264"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0349">Heme</keyword>
    <keyword evidence="1" id="KW-0408">Iron</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="16"/>
            <end position="32"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF10736.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="473" mass="53199" checksum="536DF87D068EB83C" modified="2006-05-30" version="1">MTTESQAQCPFQTAKSPPPAGSPLPHPYGTWPPGPAAGLTGWHLLRRMSRDLLGTLGEWQQTYGDVVHLRMWPEHAVVVTDPQLVRELLVTHHDSLIRWERGTRVFSRVHGHSVLTAEGDAWSRKRQALQPGFMPKAVHGFVPGIVEIVDKGLATWPTRVADWPVESALTSLTMDVIVRMMFSDEIGEDARVAECAVRAISEAANADFYWPASLPDWVPWKRARRRALHTLRDLIERHLQARLRMRTDTWPDDLLSRLLCLHRDDATAWPLQAVRDECMTTFLAGHETTAATLTWWAWCMASNPSAQDAARAEVTHVLRGQAPTADSRQALRQVVQTITESMRLYPVAPVLISRRAVRPITLGPWRLPARTLFMLPLQLMHHDPRLFPEPERFQPDRFSTGSPQAPRGAYMPFGTGPRVCLGQHLATAEMTVIAAMLLQRYKLSVPEGAAHPRPLLNVTLRPDQPLWLAVTPI</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LGN9</accession>
    <name>Q1LGN9_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Acyl-CoA dehydrogenase</fullName>
            <ecNumber evidence="8">1.3.99.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">acd</name>
        <name evidence="8" type="ordered locus">Rmet_3817</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="9">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="9">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the acyl-CoA dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.3.99.-" evidence="8"/>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10687.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518333.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3817"/>
    <dbReference type="EnsemblBacteria" id="ABF10687">
        <property type="protein sequence ID" value="ABF10687"/>
        <property type="gene ID" value="Rmet_3817"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3817"/>
    <dbReference type="eggNOG" id="COG1960">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016513_0_0_4"/>
    <dbReference type="OMA" id="VEFDGTW"/>
    <dbReference type="OrthoDB" id="433393at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003995">
        <property type="term" value="F:acyl-CoA dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006089">
        <property type="entry name" value="Acyl-CoA_DH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006091">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036250">
        <property type="entry name" value="AcylCo_DH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009075">
        <property type="entry name" value="AcylCo_DH/oxidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009100">
        <property type="entry name" value="AcylCoA_DH/oxidase_NM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041504">
        <property type="entry name" value="AidB_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00441">
        <property type="entry name" value="Acyl-CoA_dh_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02770">
        <property type="entry name" value="Acyl-CoA_dh_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18158">
        <property type="entry name" value="AidB_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47203">
        <property type="entry name" value="SSF47203"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56645">
        <property type="entry name" value="SSF56645"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00072">
        <property type="entry name" value="ACYL_COA_DH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00073">
        <property type="entry name" value="ACYL_COA_DH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0274">FAD</keyword>
    <keyword evidence="3" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-0614">Plasmid</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AidB_N" evidence="7">
        <location>
            <begin position="10"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA_dh_M" evidence="6">
        <location>
            <begin position="179"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA_dh_1" evidence="5">
        <location>
            <begin position="287"/>
            <end position="442"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF02770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF18158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ABF10687.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="548" mass="60838" checksum="C5CC16333243299F" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LH06</accession>
    <name>Q1LH06_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 13">Flagellar biosynthetic protein FlhB</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13 15" type="primary">flhB</name>
        <name evidence="15" type="ordered locus">Rmet_3698</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="16">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="16">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12 13">Required for formation of the rod structure in the basal body of the flagellar apparatus. Together with FliI and FliH, may constitute the export apparatus of flagellin.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="13">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the type III secretion exporter family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10570.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518219.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3698"/>
    <dbReference type="MEROPS" id="N06.A01"/>
    <dbReference type="EnsemblBacteria" id="ABF10570">
        <property type="protein sequence ID" value="ABF10570"/>
        <property type="gene ID" value="Rmet_3698"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3698"/>
    <dbReference type="eggNOG" id="COG1377">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041013_1_0_4"/>
    <dbReference type="OMA" id="DEYKQQE"/>
    <dbReference type="OrthoDB" id="1424100at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044780">
        <property type="term" value="P:bacterial-type flagellum assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009306">
        <property type="term" value="P:protein secretion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1690.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006136">
        <property type="entry name" value="FlhB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006135">
        <property type="entry name" value="T3SS_substrate_exporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029025">
        <property type="entry name" value="T3SS_substrate_exporter_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30531">
        <property type="entry name" value="PTHR30531"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01312">
        <property type="entry name" value="Bac_export_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00950">
        <property type="entry name" value="TYPE3IMSPROT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160544">
        <property type="entry name" value="SSF160544"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00328">
        <property type="entry name" value="flhB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 13" id="KW-1005">Bacterial flagellum biogenesis</keyword>
    <keyword evidence="11 13" id="KW-1006">Bacterial flagellum protein export</keyword>
    <keyword evidence="5 13" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="15" id="KW-0966">Cell projection</keyword>
    <keyword evidence="15" id="KW-0969">Cilium</keyword>
    <keyword evidence="15" id="KW-0282">Flagellum</keyword>
    <keyword evidence="10 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-0614">Plasmid</keyword>
    <keyword evidence="8 13" id="KW-0653">Protein transport</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 13" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4 13" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="33"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="89"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="189"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00021622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022795"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00025078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU364091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="ABF10570.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="380" mass="41795" checksum="5F28072224800264" modified="2006-05-30" version="1">MSEESDLEKTEPATARRLEKAREEGQVVRSRELGTFAMLMTGVIGLWATGGVLGRKLDAVMHAGLAFEPATAFDTNRMLSQFAAIVWDALLAFLPLLLMFGVAALVTPLALGGWMFSTKSFSPDFSRMSPISGLGRLFSAHSLVELAKAIAKSLLVGGVGAWMIWRKLPEAIALMDAPIQEALLHMMELVLYVSGVVAGSLILVAALDVPWQLYTFHKKLRMTKEEIKQEMKETDGDPHIKARIRQQQRAIARRRMMAEVPKADVVVTNPTHYAVALRYEEGRMGAPRVVAKGADEVAARIRELAAEHRVPMLSAPPLARALHRHVELGHEIPAGLYTAVAEVLAWVYQLKNWHYSYGPQPNGPADLVVPDELAVPESRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LH37</accession>
    <name>Q1LH37_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">3-oxoacid CoA-transferase, subunit A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_3667</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the 3-oxoacid CoA-transferase subunit A family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10539.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3667"/>
    <dbReference type="EnsemblBacteria" id="ABF10539">
        <property type="protein sequence ID" value="ABF10539"/>
        <property type="gene ID" value="Rmet_3667"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3667"/>
    <dbReference type="eggNOG" id="COG1788">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019942_2_1_4"/>
    <dbReference type="OMA" id="HDGATVM"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008410">
        <property type="term" value="F:CoA-transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012792">
        <property type="entry name" value="3-oxoacid_CoA-transf_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004165">
        <property type="entry name" value="CoA_trans_fam_I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004163">
        <property type="entry name" value="CoA_transf_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037171">
        <property type="entry name" value="NagB/RpiA_transferase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13707">
        <property type="entry name" value="PTHR13707"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01144">
        <property type="entry name" value="CoA_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00882">
        <property type="entry name" value="CoA_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100950">
        <property type="entry name" value="SSF100950"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02429">
        <property type="entry name" value="pcaI_scoA_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01273">
        <property type="entry name" value="COA_TRANSF_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005612"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF10539.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="221" mass="23417" checksum="EE47A6E5683F3272" modified="2006-05-30" version="1">MESAMADIHDGATILIGGFGLAGMPAELIDALIAQGARDLTIVNNNAGNGDTGLAALLKAKRVRKIICSFPRQADSYVFDSLFHAGEIELELVPQGNLAERIRAAGAGIGGFFTRTAYGTPLAEGKETRIIDGQGYVFEKPIHADFALIKAETADRWGNLVYRKTARNFGPIMAMAAKCPIVQVSNVVELGELDPEHIITPGLFVKRVVKVASAAKPASAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LHC2</accession>
    <name>Q1LHC2_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Multidrug resistance efflux transporter, acriflavin resistance protein (MtdBC-AcrB/AcrD/AcrF family)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">acr</name>
        <name evidence="2" type="ordered locus">Rmet_3582</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10454.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517996.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3582"/>
    <dbReference type="EnsemblBacteria" id="ABF10454">
        <property type="protein sequence ID" value="ABF10454"/>
        <property type="gene ID" value="Rmet_3582"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3582"/>
    <dbReference type="eggNOG" id="COG0841">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002755_1_2_4"/>
    <dbReference type="OMA" id="TSYNRMS"/>
    <dbReference type="OrthoDB" id="145346at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2090.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027463">
        <property type="entry name" value="AcrB_DN_DC_subdom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001036">
        <property type="entry name" value="Acrflvin-R"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32063">
        <property type="entry name" value="PTHR32063"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00873">
        <property type="entry name" value="ACR_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00702">
        <property type="entry name" value="ACRIFLAVINRP"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82714">
        <property type="entry name" value="SSF82714"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="335"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="361"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="433"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="464"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="559"/>
            <end position="578"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="886"/>
            <end position="904"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="937"/>
            <end position="963"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="984"/>
            <end position="1003"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="1015"/>
            <end position="1041"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF10454.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="1069" mass="114635" checksum="502CB20708895A86" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-04-07" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LHH0</accession>
    <name>Q1LHH0_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Putative pterin-4-alpha-carbinolamine dehydratase</fullName>
            <shortName evidence="4">PHS</shortName>
            <ecNumber evidence="4">4.2.1.96</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">4-alpha-hydroxy-tetrahydropterin dehydratase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="4">Pterin carbinolamine dehydratase</fullName>
            <shortName evidence="4">PCD</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5" type="primary">phhB</name>
        <name evidence="5" type="ordered locus">Rmet_3534</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 4">
            <text>(4aS,6R)-4a-hydroxy-L-erythro-5,6,7,8-tetrahydrobiopterin = (6R)-L-erythro-6,7-dihydrobiopterin + H2O</text>
            <dbReference type="Rhea" id="RHEA:11920"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15642"/>
            <dbReference type="ChEBI" id="CHEBI:43120"/>
            <dbReference type="EC" id="4.2.1.96"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 4">Belongs to the pterin-4-alpha-carbinolamine dehydratase family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.96" evidence="4"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10406.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3534"/>
    <dbReference type="EnsemblBacteria" id="ABF10406">
        <property type="protein sequence ID" value="ABF10406"/>
        <property type="gene ID" value="Rmet_3534"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3534"/>
    <dbReference type="eggNOG" id="COG2154">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_081974_3_0_4"/>
    <dbReference type="OMA" id="NFAQMVG"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008124">
        <property type="term" value="F:4-alpha-hydroxytetrahydrobiopterin dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006729">
        <property type="term" value="P:tetrahydrobiopterin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1360.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00434">
        <property type="entry name" value="Pterin_4_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036428">
        <property type="entry name" value="PCD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001533">
        <property type="entry name" value="Pterin_deHydtase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12599">
        <property type="entry name" value="PTHR12599"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01329">
        <property type="entry name" value="Pterin_4a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55248">
        <property type="entry name" value="SSF55248"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4 5" id="KW-0456">Lyase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABF10406.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="125" mass="13583" checksum="FF9E8F35703CC5BC" modified="2006-05-30" version="1">MPASPAARGPGGPAFVLPYSSDRAPIMTPLSQEARASLLAGLPGWTPVQDRDAIQKSFRFADFNAAFGFMTRVALQADKVDHHPEWFNVYNRVDITLSTHDANGLTQRDIDLATFIEKAAAGLAR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-05-20" modified="2022-08-03" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LHJ9</accession>
    <name>RSMG_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Ribosomal RNA small subunit methyltransferase G</fullName>
            <ecNumber evidence="1">2.1.1.170</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">16S rRNA 7-methylguanosine methyltransferase</fullName>
            <shortName evidence="1">16S rRNA m7G methyltransferase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rsmG</name>
        <name type="ordered locus">Rmet_3505</name>
    </gene>
    <organism>
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Specifically methylates the N7 position of guanine in position 527 of 16S rRNA.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>guanosine(527) in 16S rRNA + S-adenosyl-L-methionine = N(7)-methylguanosine(527) in 16S rRNA + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:42732"/>
            <dbReference type="Rhea" id="RHEA-COMP:10209"/>
            <dbReference type="Rhea" id="RHEA-COMP:10210"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:74269"/>
            <dbReference type="ChEBI" id="CHEBI:74480"/>
            <dbReference type="EC" id="2.1.1.170"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the methyltransferase superfamily. RNA methyltransferase RsmG family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.170" evidence="1"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10377.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517929.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q1LHJ9"/>
    <dbReference type="SMR" id="Q1LHJ9"/>
    <dbReference type="STRING" id="266264.Rmet_3505"/>
    <dbReference type="EnsemblBacteria" id="ABF10377">
        <property type="protein sequence ID" value="ABF10377"/>
        <property type="gene ID" value="Rmet_3505"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3505"/>
    <dbReference type="eggNOG" id="COG0357">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_065341_2_0_4"/>
    <dbReference type="OMA" id="ICFPHLH"/>
    <dbReference type="OrthoDB" id="1590629at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070043">
        <property type="term" value="F:rRNA (guanine-N7-)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00074">
        <property type="entry name" value="16SrRNA_methyltr_G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003682">
        <property type="entry name" value="rRNA_ssu_MeTfrase_G"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31760">
        <property type="entry name" value="PTHR31760"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02527">
        <property type="entry name" value="GidB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF003078">
        <property type="entry name" value="GidB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00138">
        <property type="entry name" value="rsmG_gidB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0489">Methyltransferase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0698">rRNA processing</keyword>
    <keyword id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000335409" description="Ribosomal RNA small subunit methyltransferase G">
        <location>
            <begin position="1"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="98"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="103"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="149"/>
            <end position="150"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="164"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00074"/>
        </source>
    </evidence>
    <sequence length="235" mass="25290" checksum="EE5A60B450F34606" modified="2006-05-30" version="1">MGGSRHFAVDDAAQRRRLEAGLDAIGLALTPAQVDTLFAYLTLLRKWNGVYNLTAIRHPDEMLTHHLLDSLTAVPALAEAARSANVAQGARGRVLDVGSGGGMPGMPLAISCPDVSVLMVDIVQKKTAFLTQCRAQLHLTNAAAHWGPVEKIDDEQGYAVITSRAFAELTDFVTLSGHLLAPGGKLIAMKGVYPQAEIDRMEAAGLMADWQVEAVPKLVVPELDVERHLVVLSRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="109" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LHL4</accession>
    <name>Q1LHL4_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Primosomal protein N'</fullName>
            <ecNumber evidence="9">3.6.4.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">ATP-dependent helicase PriA</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9 11" type="primary">priA</name>
        <name evidence="11" type="ordered locus">Rmet_3490</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Involved in the restart of stalled replication forks. Recognizes and binds the arrested nascent DNA chain at stalled replication forks. It can open the DNA duplex, via its helicase activity, and promote assembly of the primosome and loading of the major replicative helicase DnaB onto DNA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Component of the primosome.</text>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the helicase family. PriA subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.-" evidence="9"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10362.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517918.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3490"/>
    <dbReference type="EnsemblBacteria" id="ABF10362">
        <property type="protein sequence ID" value="ABF10362"/>
        <property type="gene ID" value="Rmet_3490"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3490"/>
    <dbReference type="eggNOG" id="COG1198">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013353_4_0_4"/>
    <dbReference type="OMA" id="RCHYCGY"/>
    <dbReference type="OrthoDB" id="1132322at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990077">
        <property type="term" value="C:primosome complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003678">
        <property type="term" value="F:DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006269">
        <property type="term" value="P:DNA replication, synthesis of RNA primer"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006268">
        <property type="term" value="P:DNA unwinding involved in DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1440.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00983">
        <property type="entry name" value="PriA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005259">
        <property type="entry name" value="PriA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041222">
        <property type="entry name" value="PriA_3primeBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042115">
        <property type="entry name" value="PriA_3primeBD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041236">
        <property type="entry name" value="PriA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040498">
        <property type="entry name" value="PriA_CRR"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17764">
        <property type="entry name" value="PriA_3primeBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18074">
        <property type="entry name" value="PriA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18319">
        <property type="entry name" value="PriA_CRR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00595">
        <property type="entry name" value="priA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 9" id="KW-0235">DNA replication</keyword>
    <keyword evidence="8 9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6 9" id="KW-0347">Helicase</keyword>
    <keyword evidence="5 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="1 9" id="KW-0639">Primosome</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <keyword evidence="9" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="10">
        <location>
            <begin position="234"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C4-type" evidence="9">
        <location>
            <begin position="468"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C4-type" evidence="9">
        <location>
            <begin position="495"/>
            <end position="511"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ABF10362.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="764" mass="83936" checksum="A4AB56BC6286B051" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="103" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LHP3</accession>
    <name>Q1LHP3_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Polyamine aminopropyltransferase</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">Putrescine aminopropyltransferase</fullName>
            <shortName evidence="4">PAPT</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="4">Spermidine synthase</fullName>
            <shortName evidence="4">SPDS</shortName>
            <shortName evidence="4">SPDSY</shortName>
            <ecNumber evidence="4">2.5.1.16</ecNumber>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="4" type="primary">speE</name>
        <name evidence="7" type="ordered locus">Rmet_3461</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Catalyzes the irreversible transfer of a propylamine group from the amino donor S-adenosylmethioninamine (decarboxy-AdoMet) to putrescine (1,4-diaminobutane) to yield spermidine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>putrescine + S-adenosyl 3-(methylsulfanyl)propylamine = H(+) + S-methyl-5'-thioadenosine + spermidine</text>
            <dbReference type="Rhea" id="RHEA:12721"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17509"/>
            <dbReference type="ChEBI" id="CHEBI:57443"/>
            <dbReference type="ChEBI" id="CHEBI:57834"/>
            <dbReference type="ChEBI" id="CHEBI:326268"/>
            <dbReference type="EC" id="2.5.1.16"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="4">Amine and polyamine biosynthesis; spermidine biosynthesis; spermidine from putrescine: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homodimer or homotetramer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cell membrane</location>
            <topology evidence="4">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the spermidine/spermine synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.16" evidence="4"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10333.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517891.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3461"/>
    <dbReference type="EnsemblBacteria" id="ABF10333">
        <property type="protein sequence ID" value="ABF10333"/>
        <property type="gene ID" value="Rmet_3461"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3461"/>
    <dbReference type="eggNOG" id="COG4262">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_034289_1_0_4"/>
    <dbReference type="OMA" id="SFGEWGY"/>
    <dbReference type="OrthoDB" id="1613081at2"/>
    <dbReference type="UniPathway" id="UPA00248">
        <property type="reaction ID" value="UER00314"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004766">
        <property type="term" value="F:spermidine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008295">
        <property type="term" value="P:spermidine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00198">
        <property type="entry name" value="Spermidine_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030374">
        <property type="entry name" value="PABS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030373">
        <property type="entry name" value="PABS_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001045">
        <property type="entry name" value="Spermi_synthase"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="SSF103473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01330">
        <property type="entry name" value="PABS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51006">
        <property type="entry name" value="PABS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 4 5" id="KW-0620">Polyamine biosynthesis</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0745">Spermidine biosynthesis</keyword>
    <keyword evidence="1 4 5" id="KW-0808">Transferase</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="35"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="69"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="100"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="141"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="167"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="197"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="domain" description="PABS" evidence="6">
        <location>
            <begin position="199"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="region of interest" description="S-methyl-5'-thioadenosine binding" evidence="4">
        <location>
            <begin position="346"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="4 5">
        <location>
            <position position="364"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="238"/>
        </location>
        <ligand>
            <name>S-methyl-5'-thioadenosine</name>
            <dbReference type="ChEBI" id="CHEBI:17509"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="268"/>
        </location>
        <ligand>
            <name>spermidine</name>
            <dbReference type="ChEBI" id="CHEBI:57834"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="292"/>
        </location>
        <ligand>
            <name>spermidine</name>
            <dbReference type="ChEBI" id="CHEBI:57834"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="312"/>
        </location>
        <ligand>
            <name>S-methyl-5'-thioadenosine</name>
            <dbReference type="ChEBI" id="CHEBI:17509"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00354"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABF10333.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="503" mass="56757" checksum="82E736B65A982B77" modified="2006-05-30" version="1">MRDRTLVLSVLIVASCGLGYELIAGALSSYLLGDSILQFSSIIGCYLFAMGVGSWLSRYVKDEDVLTRFIDIEILVGLLGGISAALLFVVFAWLSAPFRTALYALVFVIGMLVGMEIPLVMRILNARRAQFSDLVSRVLTFDYLGALAVSLVFPLVLAPRLGLSRTGFLFGMLNVAVALFTLRIFRDEVSRTTLRTLRAGMVLAVLTVGFAGSNYLTHWAERGLFGDEIIHSETTPYQRLVITRWKDDLRLYINGNLQFSSRDEHRYHEALVHPVLQALPWARRVLVIGGGDGLALREILKYRNIEHVTLVDLDPAMTTLFSRSEPLRALNHGSLTDQRVTVVNADAAQWLEQNQDSFDAIIVDLPDPSNFGLGKLYSVPMYRLMSRHLAEKGYAVVQSTSPYYAPRSFWDIDATLREAGLHTWPYHTYVPSFGEWGYILVGKRNDYTPPTRYDVPMKFLDGDTTALMFRFPADMAPRQGEPNRLNEQTLVHDFERDWRNVIR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-01-15" modified="2022-08-03" version="99" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LIA4</accession>
    <name>MURA_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">UDP-N-acetylglucosamine 1-carboxyvinyltransferase</fullName>
            <ecNumber evidence="1">2.5.1.7</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Enoylpyruvate transferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">UDP-N-acetylglucosamine enolpyruvyl transferase</fullName>
            <shortName evidence="1">EPT</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">murA</name>
        <name type="ordered locus">Rmet_3250</name>
    </gene>
    <organism>
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Cell wall formation. Adds enolpyruvyl to UDP-N-acetylglucosamine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>phosphoenolpyruvate + UDP-N-acetyl-alpha-D-glucosamine = phosphate + UDP-N-acetyl-3-O-(1-carboxyvinyl)-alpha-D-glucosamine</text>
            <dbReference type="Rhea" id="RHEA:18681"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57705"/>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
            <dbReference type="ChEBI" id="CHEBI:68483"/>
            <dbReference type="EC" id="2.5.1.7"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cell wall biogenesis; peptidoglycan biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the EPSP synthase family. MurA subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.7" evidence="1"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10122.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517724.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q1LIA4"/>
    <dbReference type="SMR" id="Q1LIA4"/>
    <dbReference type="STRING" id="266264.Rmet_3250"/>
    <dbReference type="EnsemblBacteria" id="ABF10122">
        <property type="protein sequence ID" value="ABF10122"/>
        <property type="gene ID" value="Rmet_3250"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3250"/>
    <dbReference type="eggNOG" id="COG0766">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027387_0_0_4"/>
    <dbReference type="OMA" id="CDPHRAT"/>
    <dbReference type="OrthoDB" id="537477at2"/>
    <dbReference type="UniPathway" id="UPA00219"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008760">
        <property type="term" value="F:UDP-N-acetylglucosamine 1-carboxyvinyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007049">
        <property type="term" value="P:cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009252">
        <property type="term" value="P:peptidoglycan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008360">
        <property type="term" value="P:regulation of cell shape"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019277">
        <property type="term" value="P:UDP-N-acetylgalactosamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01555">
        <property type="entry name" value="UdpNAET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.65.10.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00111">
        <property type="entry name" value="MurA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001986">
        <property type="entry name" value="Enolpyruvate_Tfrase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036968">
        <property type="entry name" value="Enolpyruvate_Tfrase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013792">
        <property type="entry name" value="RNA3'P_cycl/enolpyr_Trfase_a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005750">
        <property type="entry name" value="UDP_GlcNAc_COvinyl_MurA"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00275">
        <property type="entry name" value="EPSP_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55205">
        <property type="entry name" value="SSF55205"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01072">
        <property type="entry name" value="murA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0131">Cell cycle</keyword>
    <keyword id="KW-0132">Cell division</keyword>
    <keyword id="KW-0133">Cell shape</keyword>
    <keyword id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0573">Peptidoglycan synthesis</keyword>
    <keyword id="KW-0670">Pyruvate</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_1000023080" description="UDP-N-acetylglucosamine 1-carboxyvinyltransferase">
        <location>
            <begin position="1"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="1">
        <location>
            <position position="116"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="22"/>
            <end position="23"/>
        </location>
        <ligand>
            <name>phosphoenolpyruvate</name>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="92"/>
        </location>
        <ligand>
            <name>UDP-N-acetyl-alpha-D-glucosamine</name>
            <dbReference type="ChEBI" id="CHEBI:57705"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="121"/>
            <end position="125"/>
        </location>
        <ligand>
            <name>UDP-N-acetyl-alpha-D-glucosamine</name>
            <dbReference type="ChEBI" id="CHEBI:57705"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="304"/>
        </location>
        <ligand>
            <name>UDP-N-acetyl-alpha-D-glucosamine</name>
            <dbReference type="ChEBI" id="CHEBI:57705"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="326"/>
        </location>
        <ligand>
            <name>UDP-N-acetyl-alpha-D-glucosamine</name>
            <dbReference type="ChEBI" id="CHEBI:57705"/>
        </ligand>
    </feature>
    <feature type="modified residue" description="2-(S-cysteinyl)pyruvic acid O-phosphothioketal" evidence="1">
        <location>
            <position position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00111"/>
        </source>
    </evidence>
    <sequence length="416" mass="44218" checksum="5C3AB414F11A86DA" modified="2006-05-30" version="1">MDKFQIQGNGPLKGEIRISGAKNAALPILCAGLLTADTVTIGNVPDLQDTRTMLKLLRQMGMKAEMVDGVATLQGADINSPEASYDLVKTMRASILVLGPLVARFGEARVSLPGGCGIGARPVDQHIKGLQAMGAEITIEHGFIHARANRLKGARVVTDMITVTGTENLLMAATLAEGETVLENAAREPEVTDLAELLVKMGAKIEGIGTDRLVVQGVDRLHGAEHKVVADRIEAGTFLCAAAATLGDIVLRGIPPLILDAVLIKLREAGATVETGDDWIRLAMPQRAQAVSFRTSEYPAFPTDMQAQFMALNAVAEGTARITETIFENRFMHVQELNRLGANITAEGNTAVVTGVPRLSGASVMATDLRASASLVIAGLVADGETVIDRIYHLDRGYDRMENKLSAVGAKILRIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LIB6</accession>
    <name>Q1LIB6_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Diadenosine tetraphosphate (Ap4A) hydrolase or other HIT (Histidine triad) family hydrolase</fullName>
            <ecNumber evidence="3">3.6.1.17</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_3238</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EC" id="3.6.1.17" evidence="3"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10110.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008643063.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3238"/>
    <dbReference type="EnsemblBacteria" id="ABF10110">
        <property type="protein sequence ID" value="ABF10110"/>
        <property type="gene ID" value="Rmet_3238"/>
    </dbReference>
    <dbReference type="GeneID" id="60825562"/>
    <dbReference type="KEGG" id="rme:Rmet_3238"/>
    <dbReference type="eggNOG" id="COG0537">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_056776_8_1_4"/>
    <dbReference type="OMA" id="YHLHAHL"/>
    <dbReference type="OrthoDB" id="1934382at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004081">
        <property type="term" value="F:bis(5'-nucleosyl)-tetraphosphatase (asymmetrical) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.428.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019808">
        <property type="entry name" value="Histidine_triad_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001310">
        <property type="entry name" value="Histidine_triad_HIT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011146">
        <property type="entry name" value="HIT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036265">
        <property type="entry name" value="HIT-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23089">
        <property type="entry name" value="PTHR23089"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01230">
        <property type="entry name" value="HIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00332">
        <property type="entry name" value="HISTRIAD"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54197">
        <property type="entry name" value="SSF54197"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00892">
        <property type="entry name" value="HIT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51084">
        <property type="entry name" value="HIT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HIT" evidence="2">
        <location>
            <begin position="8"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Histidine triad motif" evidence="1">
        <location>
            <begin position="100"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51084"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF10110.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="118" mass="12927" checksum="16DBAD8DBB37A00B" modified="2006-05-30" version="1">MKTQDNCIFCKIVAGQIPSNKVYEDEDLLAFHDIHPKAPVHFLLIPKSHVDSLADCGPGESDVLARMMLKVPELARQAGCNNGFRTVINTGTDGGQEVFHLHIHVMGGPRSQWKAPAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LIS3</accession>
    <name>Q1LIS3_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Oxidoreductase (2,5-didehydrogluconate reductase)</fullName>
            <ecNumber evidence="5">1.1.1.274</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Rmet_3081</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EC" id="1.1.1.274" evidence="5"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09953.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517581.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3081"/>
    <dbReference type="EnsemblBacteria" id="ABF09953">
        <property type="protein sequence ID" value="ABF09953"/>
        <property type="gene ID" value="Rmet_3081"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3081"/>
    <dbReference type="eggNOG" id="COG0656">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023205_2_3_4"/>
    <dbReference type="OMA" id="HCRFVNM"/>
    <dbReference type="OrthoDB" id="1035565at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050580">
        <property type="term" value="F:2,5-didehydrogluconate reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020471">
        <property type="entry name" value="AKR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023210">
        <property type="entry name" value="NADP_OxRdtase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036812">
        <property type="entry name" value="NADP_OxRdtase_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00248">
        <property type="entry name" value="Aldo_ket_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000097">
        <property type="entry name" value="AKR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00069">
        <property type="entry name" value="ALDKETRDTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51430">
        <property type="entry name" value="SSF51430"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aldo_ket_red" evidence="4">
        <location>
            <begin position="15"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="1">
        <location>
            <position position="53"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="111"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Lowers pKa of active site Tyr" evidence="3">
        <location>
            <position position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRSR" id="PIRSR000097-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR000097-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR000097-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABF09953.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="277" mass="30858" checksum="2C780E1713CD1446" modified="2006-05-30" version="1">MKQVTLPGGERVPALGMGTWNIGDHRQTRADEIATLQLGLDLGLRLIDTAEMYGEGLSESLVGEAIRGRRDQTFLVSKVYPHNASRKGAAAACERSLKRLGTDHIDLYLLHWRGDVPFEETIEAFLALRQAGKVRHFGVSNLDLSDMEELWDVPGGDEVAVNQLLYNLSRRGIEFDLLPWLREREVPVMAYSPIEQARLLRSPGLKRFAQSIGKSPAQVALAWLLSRDDIIAIPKTGNRERLRENVGALDLTLSAEQLAALDELFPPPRHVQPLAML</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LIY6</accession>
    <name>Q1LIY6_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Ferric reductase, Oxidoreductase FAD/NAD(P)-binding protein</fullName>
            <ecNumber evidence="9">1.18.1.3</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">Rmet_3017</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="1.18.1.3" evidence="9"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09890.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517535.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3017"/>
    <dbReference type="EnsemblBacteria" id="ABF09890">
        <property type="protein sequence ID" value="ABF09890"/>
        <property type="gene ID" value="Rmet_3017"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3017"/>
    <dbReference type="eggNOG" id="COG4097">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003827_19_0_4"/>
    <dbReference type="OMA" id="VGITPMR"/>
    <dbReference type="OrthoDB" id="1834577at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008860">
        <property type="term" value="F:ferredoxin-NAD+ reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017927">
        <property type="entry name" value="FAD-bd_FR_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013130">
        <property type="entry name" value="Fe3_Rdtase_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039261">
        <property type="entry name" value="FNR_nucleotide-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000951">
        <property type="entry name" value="Ph_dOase_redase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017938">
        <property type="entry name" value="Riboflavin_synthase-like_b-brl"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01794">
        <property type="entry name" value="Ferric_reduct"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00409">
        <property type="entry name" value="PHDIOXRDTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52343">
        <property type="entry name" value="SSF52343"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63380">
        <property type="entry name" value="SSF63380"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51384">
        <property type="entry name" value="FAD_FR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0408">Iron</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="40"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="80"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="142"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="172"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="197"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="domain" description="FAD-binding FR-type" evidence="8">
        <location>
            <begin position="221"/>
            <end position="321"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ABF09890.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="447" mass="50212" checksum="513C6655FBA2B693" modified="2006-05-30" version="1">MKRIIWTFWFFALALSALWLFTDTLWPAQSNYFALRTVWIQYSGVLAIGAMSVAMMLATRPAWLEPSLNGLDKIYRLHKWLGIAALTAATVHWLWAQGTKWAVGWGWLTRPARKPRATADSMGSIESALRGWRGLAEDLGEWAFYAVAVLLVLALVKRFPYRLFAKTHHWMAAIYLVLAFHTLVLVQFSYWAQPVGWALAMLLASGTVSAVWVLLGRVGAQRTAHGVIESLQTYPALNVLETTLRMDPGWPGHRPGQFAFAMSNPKEGPHPYTLASAWVPEDRRITFITKALGDHTRRLPERLRVGDQVTVEGPYGCFTFDDEKARQIWIGAGIGITPFIARMKYLAQHKGQDARPVDLFHPTSDVDPQAIEKLRADSQAAGVVLHLLIDKQNGRLTGERLRAMVPGWKDASVWFCGPTAFGRALRADLLAQGLEPEAFHQELFEMR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LJ70</accession>
    <name>Q1LJ70_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Transcriptional regulator, DeoR-family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Rmet_2933</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09806.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517476.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2933"/>
    <dbReference type="EnsemblBacteria" id="ABF09806">
        <property type="protein sequence ID" value="ABF09806"/>
        <property type="gene ID" value="Rmet_2933"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2933"/>
    <dbReference type="eggNOG" id="COG2378">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041141_7_1_4"/>
    <dbReference type="OMA" id="PDVLMTL"/>
    <dbReference type="OrthoDB" id="1654295at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001034">
        <property type="entry name" value="DeoR_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013196">
        <property type="entry name" value="HTH_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026881">
        <property type="entry name" value="WYL_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08279">
        <property type="entry name" value="HTH_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13280">
        <property type="entry name" value="WYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51000">
        <property type="entry name" value="HTH_DEOR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH deoR-type" evidence="3">
        <location>
            <begin position="4"/>
            <end position="59"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51000"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF09806.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="230" mass="25833" checksum="722C17005973A0B2" modified="2006-05-30" version="1">MSRRADRLFQIVQVLRGRRLTTAALLAQRLGVSERTVYRDIQALSLSGVPVEGEAGIGYRLRPDYDVPPLMFTSMEVEAMVAGLRLLKAWGGGALAAAADPSLEKLMAALPPQRRVAAQQSRIFAPEYVNQPAVREAFDIVHTAIGAQRLLRIDYSDAQQRESVRVVQPLGLFFWGNVWLLAAWCTTRSDYRSFRLDRCRALTMLDEHFLETSERSLNGFLRTIHSDTQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="111" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LJP2</accession>
    <name>Q1LJP2_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Formate dehydrogenase alpha subunit (Fe4S4 domain)</fullName>
            <ecNumber evidence="11">1.2.1.2</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">fdhA</name>
        <name evidence="11" type="ordered locus">Rmet_2761</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
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    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the prokaryotic molybdopterin-containing oxidoreductase family.</text>
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        <property type="gene ID" value="Rmet_2761"/>
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    <dbReference type="eggNOG" id="COG0243">
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    </dbReference>
    <dbReference type="eggNOG" id="COG3383">
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    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000422_1_1_4"/>
    <dbReference type="OMA" id="RSIQWGE"/>
    <dbReference type="OrthoDB" id="83812at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
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        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="entry name" value="Asp_de-COase-like_dom_sf"/>
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        <property type="entry name" value="Mopterin_OxRdtase"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00384">
        <property type="entry name" value="Molybdopterin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01568">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Molybdop_Fe4S4"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="4FE4S_MOW_BIS_MGD"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="MOLYBDOPTERIN_PROK_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51318">
        <property type="entry name" value="TAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="6 8" id="KW-0408">Iron</keyword>
    <keyword evidence="7 8" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="3 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="domain" description="4Fe-4S Mo/W bis-MGD-type" evidence="10">
        <location>
            <begin position="108"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="29"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="115"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="118"/>
        </location>
        <ligand>
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        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="122"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
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        <location>
            <position position="150"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010312"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01004"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51669"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ABF09634.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="1025" mass="113420" checksum="527D05A9F4A382AF" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LJW6</accession>
    <name>Q1LJW6_CUPMC</name>
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        <alternativeName>
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        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ordered locus">Rmet_2687</name>
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        <lineage>
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            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
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        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
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                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell inner membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
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            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
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        <property type="molecule type" value="Genomic_DNA"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
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    <keyword evidence="5" id="KW-0488">Methylation</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 11" id="KW-1133">Transmembrane helix</keyword>
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        <location>
            <begin position="20"/>
            <end position="38"/>
        </location>
    </feature>
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        <location>
            <begin position="50"/>
            <end position="160"/>
        </location>
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        <source>
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    </evidence>
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    </evidence>
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        <source>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00030775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
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    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF12019"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="ABF09560.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="174" mass="18493" checksum="E0AA4ED9046F9FB7" modified="2006-05-30" version="1">MRTTGHASRNAKRGATLPELLISLAISAILAIAAWPVLRNLTAEQTVAHAADRLAASLALARTIAASRRVEVRIGPLAGAHTLDGGWQLDSDMEPSLAVVRASDPCLRITLRSTAGPAGAQSLRLTPVGYSRSEQGGFLAATFQLRCHRAQRQVRLGAQGRIRICRPGIDVDCD</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="54" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LJZ0</accession>
    <name>Q1LJZ0_CUPMC</name>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
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        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
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                <person name="Van Houdt R."/>
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                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
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    <dbReference type="EnsemblBacteria" id="ABF09536">
        <property type="protein sequence ID" value="ABF09536"/>
        <property type="gene ID" value="Rmet_2663"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2663"/>
    <dbReference type="eggNOG" id="ENOG5033CEH">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1377164_0_0_4"/>
    <dbReference type="OMA" id="LHIEVIM"/>
    <dbReference type="OrthoDB" id="1466911at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="163"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="175"/>
            <end position="198"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF09536.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="198" mass="21748" checksum="64D6D8F741D5530A" modified="2006-05-30" version="1">MSRPDIGDFLLHIEVIMTEQGSSDLLLLKSAEAHKVGQRSTGSIDYEVLTDMNRQVVWLRIVGNTSRGYFSSEKIDFEKAVACVKNIQADQPFPSKLLGGAFVGRSANNAGFLVAVLRAEKLLIAATNSIHQHLVAGDWRAWKQEMLALTGIPLAAEVEAKSEAQPPIVENNSVAFPEHKEHTKRQKSGRSNGADRSR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2007-09-11" modified="2022-08-03" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LK37</accession>
    <name>TSAD_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">tRNA N6-adenosine threonylcarbamoyltransferase</fullName>
            <ecNumber evidence="1">2.3.1.234</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">N6-L-threonylcarbamoyladenine synthase</fullName>
            <shortName evidence="1">t(6)A synthase</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">t(6)A37 threonylcarbamoyladenosine biosynthesis protein TsaD</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">tRNA threonylcarbamoyladenosine biosynthesis protein TsaD</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">tsaD</name>
        <name type="synonym">gcp</name>
        <name type="ordered locus">Rmet_2612</name>
    </gene>
    <organism>
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t(6)A37) in tRNAs that read codons beginning with adenine. Is involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37, together with TsaE and TsaB. TsaD likely plays a direct catalytic role in this reaction.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>adenosine(37) in tRNA + L-threonylcarbamoyladenylate = AMP + H(+) + N(6)-L-threonylcarbamoyladenosine(37) in tRNA</text>
            <dbReference type="Rhea" id="RHEA:37059"/>
            <dbReference type="Rhea" id="RHEA-COMP:10162"/>
            <dbReference type="Rhea" id="RHEA-COMP:10163"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:73682"/>
            <dbReference type="ChEBI" id="CHEBI:74411"/>
            <dbReference type="ChEBI" id="CHEBI:74418"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="2.3.1.234"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
        <text evidence="1">Binds 1 Fe(2+) ion per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the KAE1 / TsaD family.</text>
    </comment>
    <comment type="sequence caution" evidence="2">
        <conflict type="erroneous initiation">
            <sequence resource="EMBL-CDS" id="ABF09489" version="1"/>
        </conflict>
    </comment>
    <dbReference type="EC" id="2.3.1.234" evidence="1"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09489.1"/>
        <property type="status" value="ALT_INIT"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008649849.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q1LK37"/>
    <dbReference type="SMR" id="Q1LK37"/>
    <dbReference type="STRING" id="266264.Rmet_2612"/>
    <dbReference type="EnsemblBacteria" id="ABF09489">
        <property type="protein sequence ID" value="ABF09489"/>
        <property type="gene ID" value="Rmet_2612"/>
    </dbReference>
    <dbReference type="GeneID" id="60820952"/>
    <dbReference type="KEGG" id="rme:Rmet_2612"/>
    <dbReference type="eggNOG" id="COG0533">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023208_6_0_4"/>
    <dbReference type="OrthoDB" id="1257362at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061711">
        <property type="term" value="F:N(6)-L-threonylcarbamoyladenine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002949">
        <property type="term" value="P:tRNA threonylcarbamoyladenosine modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01445">
        <property type="entry name" value="TsaD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000905">
        <property type="entry name" value="Gcp-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017861">
        <property type="entry name" value="KAE1/TsaD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022450">
        <property type="entry name" value="TsaD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11735">
        <property type="entry name" value="PTHR11735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11735:SF6">
        <property type="entry name" value="PTHR11735:SF6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00814">
        <property type="entry name" value="TsaD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00789">
        <property type="entry name" value="OSIALOPTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="SSF53067"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00329">
        <property type="entry name" value="gcp_kae1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03723">
        <property type="entry name" value="T6A_TsaD_YgjD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0012">Acyltransferase</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0408">Iron</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <keyword id="KW-0819">tRNA processing</keyword>
    <feature type="chain" id="PRO_0000303505" description="tRNA N6-adenosine threonylcarbamoyltransferase">
        <location>
            <begin position="1"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="111"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="115"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="134"/>
            <end position="138"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="167"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="180"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="273"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="301"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01445"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="346" mass="36826" checksum="D596FC32FEA47A4B" modified="2007-09-11" version="2">MLVLGIESSCDETGLALYDTEAGLLAHALHSQIAMHREYGGVVPELASRDHIRRVLPLLQQVLDDAGRTRADIDAIAYTQGPGLAGALLVGASVANALGFALGVPMIGVHHLEGHLLSPLLTSAPPPFPFVALLVSGGHTQLMEVKGIGDYKLLGETLDDAAGEAFDKTAKLLGLGYPGGPEVSRLAEFGNPGAFELPRPMLHSGNLDFSFAGLKTAVLTQTRKLSNACEQDRANLARAFVDAIVDVLAAKSFAALKQTGDKRLVVAGGVGANRQLRERLNEMGKRRKVEVFYPDLAFCTDNGAMIAFAGAMRLKAAPELARHGYDYGVTPRWDLADIRLPEARTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LK45</accession>
    <name>Q1LK45_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Short-chain dehydrogenase/reductase SDR</fullName>
            <ecNumber evidence="2">1.1.1.100</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_2604</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.100" evidence="2"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09481.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517184.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2604"/>
    <dbReference type="EnsemblBacteria" id="ABF09481">
        <property type="protein sequence ID" value="ABF09481"/>
        <property type="gene ID" value="Rmet_2604"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2604"/>
    <dbReference type="eggNOG" id="COG1028">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010194_1_3_4"/>
    <dbReference type="OMA" id="HEHQPRV"/>
    <dbReference type="OrthoDB" id="1601931at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004316">
        <property type="term" value="F:3-oxoacyl-[acyl-carrier-protein] reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00061">
        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU000363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF09481.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="270" mass="28449" checksum="A78D7199ED4BDF5E" modified="2006-05-30" version="1">MELHLQGKVALVTGGGQGVGRRICLELAGEGVKVVINDYFLERAQAVQKEIEAAGGQALAVQADIGDSEQVERMFAAARERFGDVDILVNNAGVTVARRTKGGMPPTFMEMPVEDWKEVVDLNIYGVMNCCHSALPGMQRKGGGKIVNIMSEAGRVGEARLAVYSGAKAAMLGFAKAIAQEHGRDCINVNTIALGAVSHEGIANGPLHPSSTPETDDRLKKMLGAYPIARGLGRLSRPEDISGVVAFLASDRAAFITGQSLGVSGGYVML</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-10-31" modified="2022-05-25" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LK78</accession>
    <name>Y2571_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">UPF0307 protein Rmet_2571</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">Rmet_2571</name>
    </gene>
    <organism>
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0307 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09448.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517151.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q1LK78"/>
    <dbReference type="SMR" id="Q1LK78"/>
    <dbReference type="STRING" id="266264.Rmet_2571"/>
    <dbReference type="EnsemblBacteria" id="ABF09448">
        <property type="protein sequence ID" value="ABF09448"/>
        <property type="gene ID" value="Rmet_2571"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2571"/>
    <dbReference type="eggNOG" id="COG3028">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_106757_1_0_4"/>
    <dbReference type="OMA" id="QMQFVGK"/>
    <dbReference type="OrthoDB" id="1711519at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd16331">
        <property type="entry name" value="YjgA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.60.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00765">
        <property type="entry name" value="UPF0307"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023153">
        <property type="entry name" value="PSPTO4464-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006839">
        <property type="entry name" value="Ribosome-assoc_YjgA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38101">
        <property type="entry name" value="PTHR38101"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04751">
        <property type="entry name" value="DUF615"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016183">
        <property type="entry name" value="UCP016183"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF158710">
        <property type="entry name" value="SSF158710"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000257637" description="UPF0307 protein Rmet_2571">
        <location>
            <begin position="1"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="20"/>
            <end position="39"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00765"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="202" mass="22677" checksum="0AA9579EB48CE4D6" modified="2006-05-30" version="1">MPPMTRNTRNNPNGRFPGAFAPEDEDDLPKSKSQRKRDMTALQDIGAELESLPKDRLARVPMPEALADAIHAARKITSHEGKRRQMQFVGKVMRGLDDDEIATIRAALEGFKGTSKAETARMHLIERWRELLLADDDALTKFLGEHPSVDVQSVRNIIRNARKEKELAKPPKYFRELFQAIKTALEAKDADAAPDNAPEPEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LK90</accession>
    <name>Q1LK90_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Serine--based site-specific recombinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">tnpR</name>
        <name evidence="8" type="ordered locus">Rmet_2559</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09436.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517138.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2559"/>
    <dbReference type="EnsemblBacteria" id="ABF09436">
        <property type="protein sequence ID" value="ABF09436"/>
        <property type="gene ID" value="Rmet_2559"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2559"/>
    <dbReference type="eggNOG" id="COG1961">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010686_10_0_4"/>
    <dbReference type="OMA" id="PELPTSW"/>
    <dbReference type="OrthoDB" id="1791095at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000150">
        <property type="term" value="F:DNA strand exchange activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1390">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006118">
        <property type="entry name" value="Recombinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006119">
        <property type="entry name" value="Resolv_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036162">
        <property type="entry name" value="Resolvase-like_N_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00239">
        <property type="entry name" value="Resolvase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00857">
        <property type="entry name" value="Resolvase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53041">
        <property type="entry name" value="SSF53041"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00397">
        <property type="entry name" value="RECOMBINASES_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51736">
        <property type="entry name" value="RECOMBINASES_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0229">DNA integration</keyword>
    <keyword evidence="3" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Resolvase/invertase-type recombinase catalytic" evidence="7">
        <location>
            <begin position="2"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="149"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="active site" description="O-(5'-phospho-DNA)-serine intermediate" evidence="4 5">
        <location>
            <position position="10"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR606118-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51736"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ABF09436.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="222" mass="24696" checksum="63815AF5FF03B256" modified="2006-05-30" version="1">MAIRAYLRASTADQDADRARESLTKFATEQGARITTFYVENASGASLDRNELDRLIDESRSGDVLLVEGIDRLTRLTQDDWKTLKQRLASKCILICAKFVPMTHDVLQATQAQRSDWAHDAVKAAIRDLMIELAAAKAREDYEVRRERAEQGIARRKAKDAAGETDRPGYGGRKADADMHRKIIDLRKRGMSYSQIVDTLGTTRPTISRALKAAGMLGTEAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LKA6</accession>
    <name>Q1LKA6_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Multidrug resistance protein DLP12 prophage</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">emrE</name>
        <name evidence="6" type="ordered locus">Rmet_2543</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cell membrane</location>
            <topology evidence="4">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the drug/metabolite transporter (DMT) superfamily. Small multidrug resistance (SMR) (TC 2.A.7.1) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09420.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517120.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2543"/>
    <dbReference type="EnsemblBacteria" id="ABF09420">
        <property type="protein sequence ID" value="ABF09420"/>
        <property type="gene ID" value="Rmet_2543"/>
    </dbReference>
    <dbReference type="GeneID" id="60820998"/>
    <dbReference type="KEGG" id="rme:Rmet_2543"/>
    <dbReference type="eggNOG" id="COG2076">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_133067_0_2_4"/>
    <dbReference type="OMA" id="ATTAMKY"/>
    <dbReference type="OrthoDB" id="1921902at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005887">
        <property type="term" value="C:integral component of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042221">
        <property type="term" value="P:response to chemical"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000390">
        <property type="entry name" value="Small_drug/metabolite_transptr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045324">
        <property type="entry name" value="Small_multidrug_res"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30561">
        <property type="entry name" value="PTHR30561"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00893">
        <property type="entry name" value="Multi_Drug_Res"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="3 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="27"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="60"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="85"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABF09420.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="109" mass="11546" checksum="AC60A8A31CE18CC1" modified="2006-05-30" version="1">MNGYLLLALAIVAEVIATSSLKAAENFTRLVPSVLVVTGYVAAFWLLMQVMKTVPVGVAYAIWCGAGIVLVTLIASVLYRQVPDLAAWLGIGLIIAGVAVIQLFSKMSH</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-10-31" modified="2022-05-25" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LKE3</accession>
    <name>HTPG_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Chaperone protein HtpG</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Heat shock protein HtpG</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">High temperature protein G</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">htpG</name>
        <name type="ordered locus">Rmet_2506</name>
    </gene>
    <organism>
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Molecular chaperone. Has ATPase activity.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the heat shock protein 90 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09383.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008648084.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q1LKE3"/>
    <dbReference type="SMR" id="Q1LKE3"/>
    <dbReference type="STRING" id="266264.Rmet_2506"/>
    <dbReference type="PRIDE" id="Q1LKE3"/>
    <dbReference type="EnsemblBacteria" id="ABF09383">
        <property type="protein sequence ID" value="ABF09383"/>
        <property type="gene ID" value="Rmet_2506"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2506"/>
    <dbReference type="eggNOG" id="COG0326">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006684_3_0_4"/>
    <dbReference type="OMA" id="MRRMKEM"/>
    <dbReference type="OrthoDB" id="246194at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.790">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00505">
        <property type="entry name" value="HSP90"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019805">
        <property type="entry name" value="Heat_shock_protein_90_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037196">
        <property type="entry name" value="HSP90_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001404">
        <property type="entry name" value="Hsp90_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020575">
        <property type="entry name" value="Hsp90_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11528">
        <property type="entry name" value="PTHR11528"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00183">
        <property type="entry name" value="HSP90"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002583">
        <property type="entry name" value="Hsp90"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00775">
        <property type="entry name" value="HEATSHOCK90"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110942">
        <property type="entry name" value="SSF110942"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="SSF54211"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="SSF55874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00298">
        <property type="entry name" value="HSP90"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0143">Chaperone</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0346">Stress response</keyword>
    <feature type="chain" id="PRO_0000258519" description="Chaperone protein HtpG">
        <location>
            <begin position="1"/>
            <end position="633"/>
        </location>
    </feature>
    <feature type="region of interest" description="A; substrate-binding" evidence="1">
        <location>
            <begin position="1"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="region of interest" description="B" evidence="1">
        <location>
            <begin position="342"/>
            <end position="562"/>
        </location>
    </feature>
    <feature type="region of interest" description="C" evidence="1">
        <location>
            <begin position="563"/>
            <end position="633"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00505"/>
        </source>
    </evidence>
    <sequence length="633" mass="71067" checksum="63CAC5B488AADA62" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="97" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LKQ8</accession>
    <name>Q1LKQ8_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Periplasmic thioredoxin of cytochrome c-type biogenesis</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">ccmG</name>
        <name evidence="7" type="ordered locus">Rmet_2391</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the thioredoxin family. DsbE subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09268.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_003149830.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2391"/>
    <dbReference type="EnsemblBacteria" id="ABF09268">
        <property type="protein sequence ID" value="ABF09268"/>
        <property type="gene ID" value="Rmet_2391"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2391"/>
    <dbReference type="eggNOG" id="COG0526">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_042529_19_1_4"/>
    <dbReference type="OMA" id="CKEEHPL"/>
    <dbReference type="OrthoDB" id="1617952at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030288">
        <property type="term" value="C:outer membrane-bounded periplasmic space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015036">
        <property type="term" value="F:disulfide oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017004">
        <property type="term" value="P:cytochrome complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03010">
        <property type="entry name" value="TlpA_like_DsbE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004799">
        <property type="entry name" value="Periplasmic_diS_OxRdtase_DsbE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013740">
        <property type="entry name" value="Redoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017937">
        <property type="entry name" value="Thioredoxin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08534">
        <property type="entry name" value="Redoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="SSF52833"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00385">
        <property type="entry name" value="dsbE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00194">
        <property type="entry name" value="THIOREDOXIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0201">Cytochrome c-type biogenesis</keyword>
    <keyword evidence="4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Thioredoxin" evidence="6">
        <location>
            <begin position="32"/>
            <end position="174"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007758"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABF09268.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="174" mass="19156" checksum="A8DFE0FAA9E7CF63" modified="2006-05-30" version="1">MNRFLWPLLGFLVLVSFLAVGLTLKPSEVPSPLINKPAPRFSLPQLQAPDLTFSSESMIGKVWVLNVWASWCAPCLEEHPVITELASRHAVSVVGMNYKDTPQNAIAWLRRNGNAFETTVSDAHGAVGIDFGVYGVPETYVIDKAGIIRYKHTGAIDAGEMRGELLPLVRELEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LL68</accession>
    <name>Q1LL68_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">ABC-type sugar transporter, periplasmic component, probable sugar binding</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_2229</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09108.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2229"/>
    <dbReference type="EnsemblBacteria" id="ABF09108">
        <property type="protein sequence ID" value="ABF09108"/>
        <property type="gene ID" value="Rmet_2229"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2229"/>
    <dbReference type="eggNOG" id="COG1653">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039393_0_0_4"/>
    <dbReference type="OMA" id="IRDSDIH"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008643">
        <property type="term" value="P:carbohydrate transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014597">
        <property type="entry name" value="ABC_tp_sb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006059">
        <property type="entry name" value="SBP"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13416">
        <property type="entry name" value="SBP_bac_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF035859">
        <property type="entry name" value="ABC_tp_sb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0762">Sugar transport</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193596" evidence="1">
        <location>
            <begin position="27"/>
            <end position="580"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF09108.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="580" mass="64794" checksum="A1BC0988899A116B" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="100" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LL76</accession>
    <name>Q1LL76_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Alkanesulfonate transporter subunit ATP-binding component of ABC superfamily</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">ssuB1</name>
        <name evidence="9" type="ordered locus">Rmet_2221</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09100.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516928.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2221"/>
    <dbReference type="EnsemblBacteria" id="ABF09100">
        <property type="protein sequence ID" value="ABF09100"/>
        <property type="gene ID" value="Rmet_2221"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2221"/>
    <dbReference type="eggNOG" id="COG1116">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_22_4"/>
    <dbReference type="OMA" id="KRVQMAQ"/>
    <dbReference type="OrthoDB" id="1832232at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 7 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="1" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-1278">Translocase</keyword>
    <feature type="domain" description="ABC transporter" evidence="8">
        <location>
            <begin position="16"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="52"/>
            <end position="59"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ABF09100.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="262" mass="28720" checksum="738FB3202C77B898" modified="2006-05-30" version="1">MVAVLEHAPEAAGARIDIRGVSHAFGDGARRLPVLDDVSLDVAPGEFVALLGPSGCGKSTLLRLVAGLETPNAGEIRQDGTPIDHPDPSRIVVFQDPTLYPWRRVRDNVALGLQARGLLRREGHRVDAALRRVGLEAFADAFPHQLSGGMAQRVALARALVNDPRLLVLDEPLGKLDSLTRLAMQSDLVELWQRAGFSALLVTHDVEEALFLAQRVIVFSQRPAKITAQIEVDLPYPRHRGDPRLTELRHEALRHLGLDASW</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LM50</accession>
    <name>Q1LM50_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Transcriptional regulator, LysR family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">ycaN</name>
        <name evidence="3" type="ordered locus">Rmet_1897</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the LysR transcriptional regulatory family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08776.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1897"/>
    <dbReference type="EnsemblBacteria" id="ABF08776">
        <property type="protein sequence ID" value="ABF08776"/>
        <property type="gene ID" value="Rmet_1897"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1897"/>
    <dbReference type="eggNOG" id="COG0583">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039613_16_1_4"/>
    <dbReference type="OMA" id="QVEVHLF"/>
    <dbReference type="OrthoDB" id="750230at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005119">
        <property type="entry name" value="LysR_subst-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000847">
        <property type="entry name" value="Tscrpt_reg_HTH_LysR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00126">
        <property type="entry name" value="HTH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03466">
        <property type="entry name" value="LysR_substrate"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50931">
        <property type="entry name" value="HTH_LYSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH lysR-type" evidence="2">
        <location>
            <begin position="6"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF08776.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="301" mass="32937" checksum="7F50D7C27D6E1960" modified="2006-05-30" version="1">MHRTGMTELEAVLAVARRNSFRGAAQELGMSTTAVSSAVAGLEARLKVRLFNRSTRSVALTDAGLRYVERIAPALAAIKSAGEEAGSGPDVPSGTLRINAPQGAANLLLAPLFNVYAQRYPDVRIDIVSESRMIDVVAEGFDAGIRLAESVPQDMIAVPLSRDVRMLVVATPAYLEHHGTPRHPRDLLQHRSIGMRMSHGGIYQWELEHKGQKLQMDLPVRIALNEMPAIRQALLLGLGIGFISEWFIEDELKTGALVPVLAPWCPSFGGLRLYYSGHRFVPARLRALVSLVHELRLAEAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LM95</accession>
    <name>Q1LM95_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Acyl-CoA dehydrogenase</fullName>
            <ecNumber evidence="10">1.3.8.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">Rmet_1852</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the acyl-CoA dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.3.8.-" evidence="10"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08731.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516579.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1852"/>
    <dbReference type="EnsemblBacteria" id="ABF08731">
        <property type="protein sequence ID" value="ABF08731"/>
        <property type="gene ID" value="Rmet_1852"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1852"/>
    <dbReference type="eggNOG" id="COG1960">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018204_12_2_4"/>
    <dbReference type="OMA" id="CMFHMMN"/>
    <dbReference type="OrthoDB" id="357522at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016627">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-CH group of donors"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.540.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.110.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025878">
        <property type="entry name" value="Acyl-CoA_dh-like_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020953">
        <property type="entry name" value="Acyl-CoA_DH_N_bac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006091">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046373">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036250">
        <property type="entry name" value="AcylCo_DH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009075">
        <property type="entry name" value="AcylCo_DH/oxidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013786">
        <property type="entry name" value="AcylCoA_DH/ox_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037069">
        <property type="entry name" value="AcylCoA_DH/ox_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009100">
        <property type="entry name" value="AcylCoA_DH/oxidase_NM_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00441">
        <property type="entry name" value="Acyl-CoA_dh_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12806">
        <property type="entry name" value="Acyl-CoA_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02770">
        <property type="entry name" value="Acyl-CoA_dh_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02771">
        <property type="entry name" value="Acyl-CoA_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12418">
        <property type="entry name" value="AcylCoA_DH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47203">
        <property type="entry name" value="SSF47203"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56645">
        <property type="entry name" value="SSF56645"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0274">FAD</keyword>
    <keyword evidence="3" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="10" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AcylCoA_DH_N" evidence="8">
        <location>
            <begin position="4"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA_dh_N" evidence="7">
        <location>
            <begin position="40"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA_dh_M" evidence="6">
        <location>
            <begin position="161"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA_dh_1" evidence="5">
        <location>
            <begin position="300"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA_dh_C" evidence="9">
        <location>
            <begin position="490"/>
            <end position="615"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF02770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF02771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF12418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF12806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ABF08731.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="619" mass="67823" checksum="04BD0C783CCD3ACF" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-10-07" version="53" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LMR5</accession>
    <name>Q1LMR5_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_1680</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08561.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1680"/>
    <dbReference type="EnsemblBacteria" id="ABF08561">
        <property type="protein sequence ID" value="ABF08561"/>
        <property type="gene ID" value="Rmet_1680"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1680"/>
    <dbReference type="eggNOG" id="COG4727">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2131431_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014547">
        <property type="entry name" value="UCP028477"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09916">
        <property type="entry name" value="DUF2145"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF08561.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="113" mass="12805" checksum="AAFF09072162BF47" modified="2006-05-30" version="1">MNAFDDVCGLVKIRIRTAGSSKPWPPQARDATIRDRGQAQAWLEMAGYKLSEMEIGTLTRLGGWMFKANVAFDDHPDSLRFAGRIRTVTVESIETFLKTQRKGWDIFEIPGRC</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LN93</accession>
    <name>Q1LN93_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Ribulose bisphosphate carboxylase small subunit</fullName>
            <shortName evidence="3">RuBisCO small subunit</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3 5" type="primary">cbbS</name>
        <name evidence="5" type="ordered locus">Rmet_1500</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">RuBisCO catalyzes two reactions: the carboxylation of D-ribulose 1,5-bisphosphate, the primary event in carbon dioxide fixation, as well as the oxidative fragmentation of the pentose substrate. Both reactions occur simultaneously and in competition at the same active site. Although the small subunit is not catalytic it is essential for maximal activity.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Heterohexadecamer of 8 large and 8 small subunits.</text>
    </comment>
    <comment type="miscellaneous">
        <text evidence="3">The basic functional RuBisCO is composed of a large chain homodimer in a 'head-to-tail' conformation. In form I RuBisCO this homodimer is arranged in a barrel-like tetramer with the small subunits forming a tetrameric 'cap' on each end of the 'barrel'.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the RuBisCO small chain family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08383.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516246.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1500"/>
    <dbReference type="EnsemblBacteria" id="ABF08383">
        <property type="protein sequence ID" value="ABF08383"/>
        <property type="gene ID" value="Rmet_1500"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1500"/>
    <dbReference type="eggNOG" id="COG4451">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_098114_2_0_4"/>
    <dbReference type="OMA" id="WNPAIEH"/>
    <dbReference type="OrthoDB" id="1708389at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016984">
        <property type="term" value="F:ribulose-bisphosphate carboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019253">
        <property type="term" value="P:reductive pentose-phosphate cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03527">
        <property type="entry name" value="RuBisCO_small"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.190.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00859">
        <property type="entry name" value="RuBisCO_S_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024681">
        <property type="entry name" value="RuBisCO_ssu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000894">
        <property type="entry name" value="RuBisCO_ssu_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036385">
        <property type="entry name" value="RuBisCO_ssu_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31262">
        <property type="entry name" value="PTHR31262"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00101">
        <property type="entry name" value="RuBisCO_small"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00961">
        <property type="entry name" value="RuBisCO_small"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55239">
        <property type="entry name" value="SSF55239"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 3" id="KW-0113">Calvin cycle</keyword>
    <keyword evidence="2 3" id="KW-0120">Carbon dioxide fixation</keyword>
    <keyword evidence="5" id="KW-0456">Lyase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RuBisCO_small" evidence="4">
        <location>
            <begin position="18"/>
            <end position="115"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022567"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABF08383.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="118" mass="13661" checksum="A303A4DCF7AE1677" modified="2006-05-30" version="1">MSDVMDYNSRLSDPASRKFETFSYLPAMDAASIKRQVEYLVKKGWNPAIEHIEPEYLMDSYWYMWKLPMFGETDVDRVLAEAKACHKANPNNHVRLVGYDNFKQSQGAAMVVYRGKTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LNS6</accession>
    <name>Q1LNS6_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Tautomerase</fullName>
            <ecNumber evidence="3">5.3.2.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">nahJ</name>
        <name evidence="5" type="ordered locus">Rmet_1317</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the 4-oxalocrotonate tautomerase family.</text>
    </comment>
    <dbReference type="EC" id="5.3.2.-" evidence="3"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08200.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516083.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1317"/>
    <dbReference type="EnsemblBacteria" id="ABF08200">
        <property type="protein sequence ID" value="ABF08200"/>
        <property type="gene ID" value="Rmet_1317"/>
    </dbReference>
    <dbReference type="GeneID" id="57200708"/>
    <dbReference type="KEGG" id="rme:Rmet_1317"/>
    <dbReference type="eggNOG" id="COG1942">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_148073_5_2_4"/>
    <dbReference type="OMA" id="MPIVQVE"/>
    <dbReference type="OrthoDB" id="2013680at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016853">
        <property type="term" value="F:isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006725">
        <property type="term" value="P:cellular aromatic compound metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00491">
        <property type="entry name" value="4Oxalocrotonate_Tautomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.429.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004370">
        <property type="entry name" value="4-oxalocrotonate_tautomerase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014347">
        <property type="entry name" value="Tautomerase/MIF_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018191">
        <property type="entry name" value="Tautomerase_Pseudo-typ"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01361">
        <property type="entry name" value="Tautomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55331">
        <property type="entry name" value="SSF55331"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00013">
        <property type="entry name" value="taut"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0413">Isomerase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tautomerase" evidence="4">
        <location>
            <begin position="2"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor; via imino nitrogen" evidence="2">
        <location>
            <position position="2"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR618191-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU362032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABF08200.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="63" mass="7110" checksum="57208963ADDE025F" modified="2006-05-30" version="1">MPIAQLYIIEGRDAEKKERLIAEVTEAIHRAIDAPVESIRVLITEMPKEHFGIAGQSARKRGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LNX6</accession>
    <name>Q1LNX6_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Cytokinin riboside 5'-monophosphate phosphoribohydrolase</fullName>
            <ecNumber evidence="2">3.2.2.n1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_1265</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>AMP + H2O = adenine + D-ribose 5-phosphate</text>
            <dbReference type="Rhea" id="RHEA:20129"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16708"/>
            <dbReference type="ChEBI" id="CHEBI:78346"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="3.2.2.4"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the LOG family.</text>
    </comment>
    <dbReference type="EC" id="3.2.2.n1" evidence="2"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08150.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516036.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1265"/>
    <dbReference type="EnsemblBacteria" id="ABF08150">
        <property type="protein sequence ID" value="ABF08150"/>
        <property type="gene ID" value="Rmet_1265"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1265"/>
    <dbReference type="eggNOG" id="COG1611">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_058336_0_0_4"/>
    <dbReference type="OMA" id="QARHYEQ"/>
    <dbReference type="OrthoDB" id="902542at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008714">
        <property type="term" value="F:AMP nucleosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009691">
        <property type="term" value="P:cytokinin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005269">
        <property type="entry name" value="LOG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031100">
        <property type="entry name" value="LOG_fam"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03641">
        <property type="entry name" value="Lysine_decarbox"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00730">
        <property type="entry name" value="TIGR00730"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0203">Cytokinin biosynthesis</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU363015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF08150.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="288" mass="32497" checksum="F86234EF4DB090D9" modified="2006-05-30" version="1">MKPAEDLAERLRAIRESPTYRLAYEDIELLGQDELRPLRLQLELLKPERILHEQGIHSTVVVFGSARVSDAETAEARLGALERQALVTPENVGLRQELARANRRVEQARHYEQARRFSGLISARFQQQNRRDFVVVTGGGPGIMEAANRGAFEVGARSIGLNITLPHEQAPNPYMCPDLAFRFHYFALRKMHFLLHAKGLVAFPGGYRTLDELFEVLTLIQTGKMQRIPVVLVGRAFWHRVVDFDLLLDEGYVSPSDLDLFTCVDKAEEIVSALERFYVTRAAGDGAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="67" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LNY8</accession>
    <name>Q1LNY8_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative dna binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_1253</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08138.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1253"/>
    <dbReference type="EnsemblBacteria" id="ABF08138">
        <property type="protein sequence ID" value="ABF08138"/>
        <property type="gene ID" value="Rmet_1253"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1253"/>
    <dbReference type="eggNOG" id="COG3311">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_140176_9_0_4"/>
    <dbReference type="OMA" id="YGTGPIY"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009061">
        <property type="entry name" value="DNA-bd_dom_put_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041657">
        <property type="entry name" value="HTH_17"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12728">
        <property type="entry name" value="HTH_17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46955">
        <property type="entry name" value="SSF46955"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH_17" evidence="1">
        <location>
            <begin position="34"/>
            <end position="84"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF08138.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="105" mass="11846" checksum="B694A402A0179CA5" modified="2006-05-30" version="1">MAGRFQPMEVHIMRPAPLRPAATVSTAAAQPQRYLTNDEAAEYLRLSPRTLEKQRVIGGGPRFRKFGRRVMYAVADLDAWAADRSFETTSDPEYAEHHSADSRAR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-01-15" modified="2022-05-25" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LP77</accession>
    <name>RL20_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">50S ribosomal protein L20</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rplT</name>
        <name type="ordered locus">Rmet_1163</name>
    </gene>
    <organism>
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Binds directly to 23S ribosomal RNA and is necessary for the in vitro assembly process of the 50S ribosomal subunit. It is not involved in the protein synthesizing functions of that subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the bacterial ribosomal protein bL20 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08049.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008650487.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q1LP77"/>
    <dbReference type="SMR" id="Q1LP77"/>
    <dbReference type="STRING" id="266264.Rmet_1163"/>
    <dbReference type="EnsemblBacteria" id="ABF08049">
        <property type="protein sequence ID" value="ABF08049"/>
        <property type="gene ID" value="Rmet_1163"/>
    </dbReference>
    <dbReference type="GeneID" id="60822033"/>
    <dbReference type="KEGG" id="rme:Rmet_1163"/>
    <dbReference type="eggNOG" id="COG0292">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_123265_0_1_4"/>
    <dbReference type="OMA" id="GRRKNVW"/>
    <dbReference type="OrthoDB" id="1932144at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000027">
        <property type="term" value="P:ribosomal large subunit assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07026">
        <property type="entry name" value="Ribosomal_L20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1900.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00382">
        <property type="entry name" value="Ribosomal_L20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005813">
        <property type="entry name" value="Ribosomal_L20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035566">
        <property type="entry name" value="Ribosomal_protein_L20_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10986">
        <property type="entry name" value="PTHR10986"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00453">
        <property type="entry name" value="Ribosomal_L20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00062">
        <property type="entry name" value="RIBOSOMALL20"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF74731">
        <property type="entry name" value="SSF74731"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01032">
        <property type="entry name" value="rplT_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00937">
        <property type="entry name" value="RIBOSOMAL_L20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <feature type="chain" id="PRO_1000049047" description="50S ribosomal protein L20">
        <location>
            <begin position="1"/>
            <end position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00382"/>
        </source>
    </evidence>
    <sequence length="118" mass="13513" checksum="F85BECF7BC0E0E9F" modified="2006-05-30" version="1">MPRVKRGVTARARHKKVIDAAKGYRGRRNNVYRIAKQAVMRAGQYAYRDRRNKKRVFRALWIARINAATREHGMTYSVFMNGLKKASIELDRKVLSDMAIHDKPAFAAIVNQVKATVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LPH3</accession>
    <name>Q1LPH3_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_1067</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07953.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515855.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1067"/>
    <dbReference type="EnsemblBacteria" id="ABF07953">
        <property type="protein sequence ID" value="ABF07953"/>
        <property type="gene ID" value="Rmet_1067"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1067"/>
    <dbReference type="eggNOG" id="COG2518">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_056435_5_2_4"/>
    <dbReference type="OMA" id="VVTNYLW"/>
    <dbReference type="OrthoDB" id="2049098at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF07953.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="191" mass="20561" checksum="A24D1F880F135F6F" modified="2006-05-30" version="1">MTLTHPPLAAPSPWLTRWAHLIRPGGRVLDLACGSGRHSAWLAAQGFAVLAVDRDAEAIAALPSGIERRTVDLEQGAWPLADAGRFDAVVVTNYLHRPLWPHLIEALAPGGVLIYETFAAGNETVGKPSRPDFLLRPGELLEVAHDALRVVGYEDGTLDVPRTAFVQRICAVREAPGGVDGTAPPRYRLPT</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LPJ7</accession>
    <name>Q1LPJ7_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">DNA internalization-related competence protein ComEC/Rec2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Rmet_1043</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07929.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515834.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1043"/>
    <dbReference type="EnsemblBacteria" id="ABF07929">
        <property type="protein sequence ID" value="ABF07929"/>
        <property type="gene ID" value="Rmet_1043"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1043"/>
    <dbReference type="eggNOG" id="COG0658">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG2333">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010363_3_0_4"/>
    <dbReference type="OMA" id="RTDKQGA"/>
    <dbReference type="OrthoDB" id="1796527at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030420">
        <property type="term" value="P:establishment of competence for transformation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07731">
        <property type="entry name" value="ComA-like_MBL-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035681">
        <property type="entry name" value="ComA-like_MBL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004477">
        <property type="entry name" value="ComEC_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004797">
        <property type="entry name" value="Competence_ComEC/Rec2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025405">
        <property type="entry name" value="DUF4131"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001279">
        <property type="entry name" value="Metallo-B-lactamas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03772">
        <property type="entry name" value="Competence"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13567">
        <property type="entry name" value="DUF4131"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00753">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00849">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="SSF56281"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00360">
        <property type="entry name" value="ComEC_N-term"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00361">
        <property type="entry name" value="ComEC_Rec2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="27"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="51"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="267"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="310"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="381"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="446"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="475"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="domain" description="Lactamase_B" evidence="7">
        <location>
            <begin position="591"/>
            <end position="790"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ABF07929.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="851" mass="91525" checksum="3994FD38B849BE1F" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="104" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LPZ7</accession>
    <name>Q1LPZ7_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Xanthine dehydrogenase, iron-sulfur cluster and FAD-binding subunit A</fullName>
            <ecNumber evidence="5">1.17.3.2</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">xdhA</name>
        <name evidence="5" type="ordered locus">Rmet_0893</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EC" id="1.17.3.2" evidence="5"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07779.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515719.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0893"/>
    <dbReference type="EnsemblBacteria" id="ABF07779">
        <property type="protein sequence ID" value="ABF07779"/>
        <property type="gene ID" value="Rmet_0893"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0893"/>
    <dbReference type="eggNOG" id="COG4630">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001681_9_0_4"/>
    <dbReference type="OMA" id="AFHKIAK"/>
    <dbReference type="OrthoDB" id="1017413at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004854">
        <property type="term" value="F:xanthine dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004855">
        <property type="term" value="F:xanthine oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd00207">
        <property type="entry name" value="fer2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.43.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.465.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002888">
        <property type="entry name" value="2Fe-2S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036884">
        <property type="entry name" value="2Fe-2S-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036010">
        <property type="entry name" value="2Fe-2S_ferredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001041">
        <property type="entry name" value="2Fe-2S_ferredoxin-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006058">
        <property type="entry name" value="2Fe2S_fd_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016208">
        <property type="entry name" value="Ald_Oxase/xanthine_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012675">
        <property type="entry name" value="Beta-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005107">
        <property type="entry name" value="CO_DH_flav_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036683">
        <property type="entry name" value="CO_DH_flav_C_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016166">
        <property type="entry name" value="FAD-bd_PCMH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036318">
        <property type="entry name" value="FAD-bd_PCMH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016167">
        <property type="entry name" value="FAD-bd_PCMH_sub1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016169">
        <property type="entry name" value="FAD-bd_PCMH_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002346">
        <property type="entry name" value="Mopterin_DH_FAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012175">
        <property type="entry name" value="Xanth_DH_ssu_bac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014307">
        <property type="entry name" value="Xanthine_DH_ssu"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11908">
        <property type="entry name" value="PTHR11908"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03450">
        <property type="entry name" value="CO_deh_flav_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00941">
        <property type="entry name" value="FAD_binding_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00111">
        <property type="entry name" value="Fer2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01799">
        <property type="entry name" value="Fer2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036557">
        <property type="entry name" value="XdhA_RC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01092">
        <property type="entry name" value="CO_deh_flav_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47741">
        <property type="entry name" value="SSF47741"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54292">
        <property type="entry name" value="SSF54292"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55447">
        <property type="entry name" value="SSF55447"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56176">
        <property type="entry name" value="SSF56176"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02963">
        <property type="entry name" value="xanthine_xdhA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00197">
        <property type="entry name" value="2FE2S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51085">
        <property type="entry name" value="2FE2S_FER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51387">
        <property type="entry name" value="FAD_PCMH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0274">FAD</keyword>
    <keyword evidence="1" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="2 5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="2Fe-2S ferredoxin-type" evidence="3">
        <location>
            <begin position="4"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="domain" description="FAD-binding PCMH-type" evidence="4">
        <location>
            <begin position="197"/>
            <end position="370"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABF07779.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="498" mass="53981" checksum="F68FCE561617C683" modified="2006-05-30" version="1">METQTIRFYHRGQVQEVSDAPVTRTLLQYLREDVRCTGTKEGCAEGDCGACTVVIGELQDSGDVEFKAVNACIQFLPTLDGKALITVEDLRQADGALHPVQEAMVECHGSQCGFCTPGFVMSLWALYQQHTPGGEPPPRQTICDALTGNLCRCTGYRPIIDAGQRMMALPAPQADRIDPRQIADTLRNLKRGETFHYNARGQHFYAPRTAAEFGAIKAAEPNIRILAGSTDVGLWVTKQFRELGNLLYVGQVEDLNQIAEHDGFIEIGAAVTLEKAYAALNTAHPELEELWKRFASLPIRNAGTLGGNIANGSPIGDSMPALIALGTQVVLQRGDVRRVMPLEDLYLAYQKTAMVEGEFVAGLRVPVQGPQHFRTYKLSKRFDEDISAVCAAFGITVENGIVTAACIAFGGMAATPKRAVLAEDALTGKPWNEATARAGMAALGQDYTPLTDMRATADYRSRGAANLLYRFWLETRDSALPAAMVNVRAIGAGETVSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="72" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LQ43</accession>
    <name>Q1LQ43_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_0847</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07733.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515675.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0847"/>
    <dbReference type="EnsemblBacteria" id="ABF07733">
        <property type="protein sequence ID" value="ABF07733"/>
        <property type="gene ID" value="Rmet_0847"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0847"/>
    <dbReference type="eggNOG" id="COG0265">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_072546_1_0_4"/>
    <dbReference type="OMA" id="DQVNLMP"/>
    <dbReference type="OrthoDB" id="741829at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="SSF50494"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF07733.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="296" mass="32192" checksum="035DCD325309A262" modified="2006-05-30" version="1">MQVKTISEQLFFSTVRIDTVAANGSSGSGTGFFFGHKFGGDKQALFVVTNKHVVTGMREGRFSFLKQKDGQPTLGDGFNLHIGQQDWQSMWFGHPDQDVDIAICPLVPLLEFVKQHHGTDLFFRAVETSMIPTQQQLAELDAVEPVTFIGYPNGIWDSKNLLPVARRGTTASPMEVDFEGTPRFLIDASVFGGSSGSPVFILNQGSFATKDGGLAIGSRFYFVGVIAAVFFRTHLNQIIPVPIPTQVQPMAQQQEMIDLGIVFKAGTVVETIDAFLKAHNVDTTAPQVAADLAPQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LQ79</accession>
    <name>Q1LQ79_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Sulfurtransferase, predicted redox protein, regulator of disulfide-bond formation</fullName>
            <ecNumber evidence="3">2.8.1.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">sirA</name>
        <name evidence="3" type="ordered locus">Rmet_0811</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the sulfur carrier protein TusA family.</text>
    </comment>
    <dbReference type="EC" id="2.8.1.-" evidence="3"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07697.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008645216.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0811"/>
    <dbReference type="EnsemblBacteria" id="ABF07697">
        <property type="protein sequence ID" value="ABF07697"/>
        <property type="gene ID" value="Rmet_0811"/>
    </dbReference>
    <dbReference type="GeneID" id="60825297"/>
    <dbReference type="KEGG" id="rme:Rmet_0811"/>
    <dbReference type="eggNOG" id="COG0425">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_165255_1_1_4"/>
    <dbReference type="OMA" id="NDMASWA"/>
    <dbReference type="OrthoDB" id="2042068at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.110.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001455">
        <property type="entry name" value="TusA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036868">
        <property type="entry name" value="TusA-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01206">
        <property type="entry name" value="TusA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64307">
        <property type="entry name" value="SSF64307"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01148">
        <property type="entry name" value="UPF0033"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="UPF0033" evidence="2">
        <location>
            <begin position="7"/>
            <end position="31"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS01148"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF07697.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="75" mass="8400" checksum="D1BD9D103E578804" modified="2006-05-30" version="1">MDFQKEVDARGLNCPLPILRTKKALADMASGEVLKVLATDPGATRDFQAFAKQTGNELLSHAEVDKVFVFYMKRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LQB2</accession>
    <name>Q1LQB2_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Ribonuclease G</fullName>
            <ecNumber evidence="7">3.1.4.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">rng</name>
        <name evidence="7" type="ordered locus">Rmet_0778</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <dbReference type="EC" id="3.1.4.-" evidence="7"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07664.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0778"/>
    <dbReference type="EnsemblBacteria" id="ABF07664">
        <property type="protein sequence ID" value="ABF07664"/>
        <property type="gene ID" value="Rmet_0778"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0778"/>
    <dbReference type="eggNOG" id="COG1530">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003468_5_3_4"/>
    <dbReference type="OMA" id="TKGPRIS"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004540">
        <property type="term" value="F:ribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006396">
        <property type="term" value="P:RNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019307">
        <property type="entry name" value="RNA-bd_AU-1/RNase_E/G"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004659">
        <property type="entry name" value="RNase_E/G"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022967">
        <property type="entry name" value="S1_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30001">
        <property type="entry name" value="PTHR30001"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10150">
        <property type="entry name" value="RNase_E_G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00316">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00757">
        <property type="entry name" value="RNaseEG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0460">Magnesium</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="S1" evidence="6">
        <location>
            <begin position="62"/>
            <end position="141"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABF07664.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="510" mass="57486" checksum="EEE57E7F1918C9BB" modified="2006-05-30" version="1">MPGHEPGHNNKRQRIGDARLSVSIAMTEDILVNITPQETRVAIVQQAAVQELHVERTLTRGLVGNIYLGKVVRVLPGMQSAFIDVGLERAAFLHVADIWHPRDGDKNGHLAIEKTLFEGQALMVQVIKDPIGTKGARLSTQVSIAGRTLVYLPQDPHIGISQRIEGEIDREALRARVQGLVPTDERGGFIVRTIAEEASDEELGNDIAYLRKIWATIRSNATTLPAPSLLYQDLNLAQRVLRDFINDATGVIQIDSRENYQKLVEFAQEYTPAVLPRLSHYTGERPIFDLFNIDAEIEKALSRRVDLKSGGYLMIDQTEAMTTIDVNTGGYVGARNFDDTIFKTNLEAAHTIARQLRLRNLGGIIIIDFIDMENVEHRDAVLSELKRALSRDRTRITVNGFSQLGLVEMTRKRTRESLAHVLCEQCPVCTGKGQVKTPRTVCYDIFREIMRESRQFNPREFRILASQEVIDLFLEEESQHLAMLGDFIGKPISLQVESTFHQEQYDIILM</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LQT3</accession>
    <name>Q1LQT3_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Hydroxymethylglutaryl-CoA lyase</fullName>
            <ecNumber evidence="4">4.1.3.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Rmet_0607</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(3S)-hydroxy-3-methylglutaryl-CoA = acetoacetate + acetyl-CoA</text>
            <dbReference type="Rhea" id="RHEA:24404"/>
            <dbReference type="ChEBI" id="CHEBI:13705"/>
            <dbReference type="ChEBI" id="CHEBI:43074"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="EC" id="4.1.3.4"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Metabolic intermediate metabolism; (S)-3-hydroxy-3-methylglutaryl-CoA degradation; acetoacetate from (S)-3-hydroxy-3-methylglutaryl-CoA: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the HMG-CoA lyase family.</text>
    </comment>
    <dbReference type="EC" id="4.1.3.4" evidence="4"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07493.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515466.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0607"/>
    <dbReference type="EnsemblBacteria" id="ABF07493">
        <property type="protein sequence ID" value="ABF07493"/>
        <property type="gene ID" value="Rmet_0607"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0607"/>
    <dbReference type="eggNOG" id="COG0119">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022138_3_2_4"/>
    <dbReference type="OMA" id="FHNTRGT"/>
    <dbReference type="OrthoDB" id="1111394at2"/>
    <dbReference type="UniPathway" id="UPA00896">
        <property type="reaction ID" value="UER00863"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004419">
        <property type="term" value="F:hydroxymethylglutaryl-CoA lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000138">
        <property type="entry name" value="HMG_CoA_lyase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043594">
        <property type="entry name" value="HMGL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000891">
        <property type="entry name" value="PYR_CT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42738">
        <property type="entry name" value="PTHR42738"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00682">
        <property type="entry name" value="HMGL-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01062">
        <property type="entry name" value="HMG_COA_LYASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50991">
        <property type="entry name" value="PYR_CT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0456">Lyase</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pyruvate carboxyltransferase" evidence="6">
        <location>
            <begin position="7"/>
            <end position="274"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005143"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012910"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABF07493.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="310" mass="32534" checksum="AC4814D2B1C10169" modified="2006-05-30" version="1">MTIPQYVKIVEVGPRDGLQNEKAMVPTDVKVALINRLTDAGFVNIEAASFVSPKWVPQMADGADVMARITRRPGTLYSVLTPNMKGFEGAVAAGADEVVIFGAASEAFSQKNINCSIAESIARFEPVAAAAKEAGVRLRGSISCALGCPYQGEVSVHAVVDVVRRLRELGCDEIDIADTIGVGTPTRVQEVMRAAAAEFSIDRLSGHFHDTYGQALSNILASLEVGIAIFHASVAGLGGCPYAKGATGNVATEDVLYMLHGMGLHTGIELDDVVRAGDFISQAIGRANSSRVGRALLTKWASQAGEASCA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LR37</accession>
    <name>Q1LR37_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Fructose-1,6-bisphosphate aldolase</fullName>
            <shortName evidence="8">FBP aldolase</shortName>
            <ecNumber evidence="8">4.1.2.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">cbbA3</name>
        <name evidence="9" type="ordered locus">Rmet_0503</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2 8">Catalyzes the aldol condensation of dihydroxyacetone phosphate (DHAP or glycerone-phosphate) with glyceraldehyde 3-phosphate (G3P) to form fructose 1,6-bisphosphate (FBP) in gluconeogenesis and the reverse reaction in glycolysis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 8">
            <text>beta-D-fructose 1,6-bisphosphate = D-glyceraldehyde 3-phosphate + dihydroxyacetone phosphate</text>
            <dbReference type="Rhea" id="RHEA:14729"/>
            <dbReference type="ChEBI" id="CHEBI:32966"/>
            <dbReference type="ChEBI" id="CHEBI:57642"/>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
            <dbReference type="EC" id="4.1.2.13"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="7">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="7">Binds 2 Zn(2+) ions per subunit. One is catalytic and the other provides a structural contribution.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="8">One is catalytic and the other provides a structural contribution.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3 8">Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 4/4.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 8">Belongs to the class II fructose-bisphosphate aldolase family.</text>
    </comment>
    <dbReference type="EC" id="4.1.2.13" evidence="8"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07389.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008652148.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0503"/>
    <dbReference type="EnsemblBacteria" id="ABF07389">
        <property type="protein sequence ID" value="ABF07389"/>
        <property type="gene ID" value="Rmet_0503"/>
    </dbReference>
    <dbReference type="GeneID" id="60824100"/>
    <dbReference type="KEGG" id="rme:Rmet_0503"/>
    <dbReference type="eggNOG" id="COG0191">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_040088_0_0_4"/>
    <dbReference type="OMA" id="PRTWGKL"/>
    <dbReference type="OrthoDB" id="827430at2"/>
    <dbReference type="UniPathway" id="UPA00109">
        <property type="reaction ID" value="UER00183"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004332">
        <property type="term" value="F:fructose-bisphosphate aldolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006096">
        <property type="term" value="P:glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00947">
        <property type="entry name" value="TBP_aldolase_IIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000771">
        <property type="entry name" value="FBA_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006412">
        <property type="entry name" value="Fruct_bisP_Calv"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01116">
        <property type="entry name" value="F_bP_aldolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001359">
        <property type="entry name" value="F_bP_aldolase_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00167">
        <property type="entry name" value="cbbA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01521">
        <property type="entry name" value="FruBisAldo_II_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00602">
        <property type="entry name" value="ALDOLASE_CLASS_II_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00806">
        <property type="entry name" value="ALDOLASE_CLASS_II_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="8 9" id="KW-0456">Lyase</keyword>
    <keyword evidence="7 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 8" id="KW-0862">Zinc</keyword>
    <feature type="region of interest" description="Dihydroxyacetone phosphate binding" evidence="6">
        <location>
            <begin position="233"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="region of interest" description="Dihydroxyacetone phosphate binding" evidence="6">
        <location>
            <begin position="275"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="5">
        <location>
            <position position="83"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="84"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="105"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="142"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="198"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="199"/>
        </location>
        <ligand>
            <name>dihydroxyacetone phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:57642"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="232"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005812"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR001359-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR001359-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR001359-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU365019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ABF07389.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="354" mass="38511" checksum="BA056FE9787FB913" modified="2006-05-30" version="1">MPLVSMRQLLDHAAENNYGLPAFNVNNLEQVQAIMQAADEVNAPVIMQASAGARKYAGEHFLRHLIEAAVEAYPHIPVVMHQDHGQSPAICKAAIDLNFSSVMMDGSLKEDGKTPSDYEYNVDVTRKVVQMAHALGVTVEGELGCLGSLETGEAGEEDGIGAEGKLDHSMLLTDPEQAADFVKATQLDALAIAIGTSHGAYKFTRKPTGDILAIGRIKEIHARIPNTHLVMHGSSSVPQELLAEIRQFGGDMKETYGVPVEEIQEAIKYGVRKINIDTDIRLAMTGAIRRFFVENPSKFDPREYLKPAREAAKLVCKARYIAFGCEGQASKIKAVSLTDVAQQYKAGKLAQVVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="72" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LRK2</accession>
    <name>Q1LRK2_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Beta-lactamase-like protein</fullName>
            <ecNumber evidence="2">3.5.2.6</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_0338</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EC" id="3.5.2.6" evidence="2"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07224.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008645261.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0338"/>
    <dbReference type="EnsemblBacteria" id="ABF07224">
        <property type="protein sequence ID" value="ABF07224"/>
        <property type="gene ID" value="Rmet_0338"/>
    </dbReference>
    <dbReference type="GeneID" id="60823940"/>
    <dbReference type="KEGG" id="rme:Rmet_0338"/>
    <dbReference type="eggNOG" id="COG0491">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_056342_4_0_4"/>
    <dbReference type="OMA" id="NVGPGHT"/>
    <dbReference type="OrthoDB" id="775108at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008800">
        <property type="term" value="F:beta-lactamase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001279">
        <property type="entry name" value="Metallo-B-lactamas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00753">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00849">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="SSF56281"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Lactamase_B" evidence="1">
        <location>
            <begin position="33"/>
            <end position="222"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF07224.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="319" mass="34963" checksum="01864A0A7F9B203C" modified="2006-05-30" version="1">MAKAFASQADLEAKQITFTQLSENAYAYTAEGDPNSGVIIGDDGVMIIDTTATPAMAQDLIAKIRAITDKPIKYVVLSHYHAVRVLGASAYFAEGAQQIIASRGTYEMIVERGEADMKSEIERFPRLFAGVETVPGLTWPTLVFEREITLFLGKLEVRIAHLGAGHTKGDTVVWLPQQKVLFSGDLVEYDAACYCGDAQLEQWPATLDALAALQPEKLVPGRGPALTTREDVAKGLAYTKDFVSTLFQAGKEAVAGKMDLKAAMAHTRKSMDPKFGQVFIYEHCLPFDVSRAFDEAAGVTHPRIWTAQRDQEMWAALQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="102" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LRQ9</accession>
    <name>Q1LRQ9_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Signal recognition particle receptor FtsY</fullName>
            <shortName evidence="5">SRP receptor</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5 8" type="primary">ftsY</name>
        <name evidence="8" type="ordered locus">Rmet_0281</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Involved in targeting and insertion of nascent membrane proteins into the cytoplasmic membrane. Acts as a receptor for the complex formed by the signal recognition particle (SRP) and the ribosome-nascent chain (RNC). Interaction with SRP-RNC leads to the transfer of the RNC complex to the Sec translocase for insertion into the membrane, the hydrolysis of GTP by both Ffh and FtsY, and the dissociation of the SRP-FtsY complex into the individual components.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Part of the signal recognition particle protein translocation system, which is composed of SRP and FtsY. SRP is a ribonucleoprotein composed of Ffh and a 4.5S RNA molecule.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cell inner membrane</location>
            <topology evidence="5">Peripheral membrane protein</topology>
            <orientation evidence="5">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the GTP-binding SRP family. FtsY subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07167.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0281"/>
    <dbReference type="EnsemblBacteria" id="ABF07167">
        <property type="protein sequence ID" value="ABF07167"/>
        <property type="gene ID" value="Rmet_0281"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0281"/>
    <dbReference type="eggNOG" id="COG0552">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009301_3_0_4"/>
    <dbReference type="OMA" id="GISDQFQ"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031226">
        <property type="term" value="C:intrinsic component of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006614">
        <property type="term" value="P:SRP-dependent cotranslational protein targeting to membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00920">
        <property type="entry name" value="FtsY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013822">
        <property type="entry name" value="Signal_recog_particl_SRP54_hlx"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004390">
        <property type="entry name" value="SR_rcpt_FtsY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036225">
        <property type="entry name" value="SRP/SRP_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000897">
        <property type="entry name" value="SRP54_GTPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042101">
        <property type="entry name" value="SRP54_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43134:SF7">
        <property type="entry name" value="PTHR43134:SF7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00448">
        <property type="entry name" value="SRP54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02881">
        <property type="entry name" value="SRP54_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00962">
        <property type="entry name" value="SRP54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00963">
        <property type="entry name" value="SRP54_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47364">
        <property type="entry name" value="SSF47364"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00064">
        <property type="entry name" value="ftsY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00300">
        <property type="entry name" value="SRP54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="8" id="KW-0132">Cell division</keyword>
    <keyword evidence="5" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 5" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="2 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4 5 8" id="KW-0675">Receptor</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SRP54" evidence="7">
        <location>
            <begin position="313"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="147"/>
            <end position="154"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="228"/>
            <end position="232"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="292"/>
            <end position="295"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004287"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00920"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS00300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ABF07167.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="340" mass="36445" checksum="F61965830D7C4CAB" modified="2006-05-30" version="1">MAPPVAPPVVPPVASAPPPASYTPPVIEELELTPPPAQTAEAKRGWMSRLRAGLSKTSRNIGVLFVGVKVDEALFEELETALLMADAGVEATEYLLGELRRRIKNDRIETAEGVKAALKDLLTQLLKPLEKTMELGREQPLVMMIAGVNGAGKTTSIGKLCKHFQSYHQSVLLAAGDTFRAAAREQLTIWGERNNVTVVAQESGDPAAVIFDAVNAARARKIDIVMADTAGRLPTQLHLMEELKKVKRVIGKSMPTAPHEVLLVIDANTGQNALAQTRAFDDALGLTGLIVTKLDGTAKGGILAAIARQRPVPVYFIGVGEKVEDLQPFKADEFAEALLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LS43</accession>
    <name>Q1LS43_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Peptidase family M48</fullName>
            <ecNumber evidence="8">3.4.-.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Rmet_0147</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="6">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the peptidase M48 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.-.-" evidence="8"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07033.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0147"/>
    <dbReference type="EnsemblBacteria" id="ABF07033">
        <property type="protein sequence ID" value="ABF07033"/>
        <property type="gene ID" value="Rmet_0147"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0147"/>
    <dbReference type="eggNOG" id="COG0501">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029002_4_0_4"/>
    <dbReference type="OMA" id="QWEVNLI"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001915">
        <property type="entry name" value="Peptidase_M48"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01435">
        <property type="entry name" value="Peptidase_M48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 6" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="1 6" id="KW-0645">Protease</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Peptidase_M48" evidence="7">
        <location>
            <begin position="82"/>
            <end position="236"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ABF07033.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="284" mass="31605" checksum="071BC56D84D89EC6" modified="2006-05-30" version="1">MVSAPDDGVRVDRRYSKVRDIVPMEVIDQQAAREYEQVKQEAIAKHTLVDEDDPQVKRLREIGIRLLPQTVLWNPRAGRWAWEINLIRSKQVNAFCMPGGKMAVYSGLLDELNLTDDEVAMVMGHEISHALEEHARQRAAQAEISNLGANVVSQLTGFGNLGNMDIGSNAKMLSLRFSRQEETEADLIGMDIAARAGYDPRAAITLWQKMGAVLKAGDEFTSTHPSGRSRIAVLQAHMNEVLPLYAKAMNMPMEKLPPYKANMADLHDAPVDAGDEDLQHPLKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="101" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LS54</accession>
    <name>Q1LS54_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Histidine kinase</fullName>
            <ecNumber evidence="2">2.7.13.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">regB</name>
        <name evidence="6" type="ordered locus">Rmet_0136</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.</text>
            <dbReference type="EC" id="2.7.13.3"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.7.13.3" evidence="2"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07022.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515053.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0136"/>
    <dbReference type="EnsemblBacteria" id="ABF07022">
        <property type="protein sequence ID" value="ABF07022"/>
        <property type="gene ID" value="Rmet_0136"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0136"/>
    <dbReference type="eggNOG" id="COG0642">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046130_1_1_4"/>
    <dbReference type="OMA" id="LNRWHLM"/>
    <dbReference type="OrthoDB" id="1755994at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00082">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003661">
        <property type="entry name" value="HisK_dim/P"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004358">
        <property type="entry name" value="Sig_transdc_His_kin-like_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00344">
        <property type="entry name" value="BCTRLSENSOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00388">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="SSF55874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="35"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="64"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="105"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="139"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="174"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="5">
        <location>
            <begin position="230"/>
            <end position="445"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABF07022.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="459" mass="49734" checksum="95EE9E7CF45C0680" modified="2006-05-30" version="1">MHPPIVMSTLPPSVAPPIGTAPQRHSLVSLRRLSWLRWALLAGQALLLVSCESLAGIALPYKPLLFLFAVQTVFNVFTVARLHWHTRHGTAPSDAELMVQMMVDLTALSAILFYTGGATNPFVSFYLPGLAIASAILPWRLVVTLAAYALACYSMLLLEYIPLDLHNPDNAVNYHLAGMWLNFVASAVMIAFFVARLSNALRQRDAQLNEARELLLREARVEALNDQAAAVAHEIGTPLATLAVIAGELRADAENTNRSDTPVADYQADLRTMEQQLELCRSILARLRADHETLAPQRIDAWLAPFVERWQLRHPQASLQAGAAPDAAAQAVDTARISQILTIALDNAARSQQAAGHDHRQGPPLRLDIAREADALVYRVTDHGHGLPPGLRARLGESPVASAHGGQGIGLYLAQSAARQLGGQISWHDNPAGGTVLELRLPLTAASERAIAAQPDFQP</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-07-28" modified="2022-08-03" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LS75</accession>
    <name>BIOF_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">8-amino-7-oxononanoate synthase</fullName>
            <shortName evidence="1">AONS</shortName>
            <ecNumber evidence="1">2.3.1.47</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">7-keto-8-amino-pelargonic acid synthase</fullName>
            <shortName evidence="1">7-KAP synthase</shortName>
            <shortName evidence="1">KAPA synthase</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">8-amino-7-ketopelargonate synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">bioF</name>
        <name type="ordered locus">Rmet_0115</name>
    </gene>
    <organism>
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the decarboxylative condensation of pimeloyl-[acyl-carrier protein] and L-alanine to produce 8-amino-7-oxononanoate (AON), [acyl-carrier protein], and carbon dioxide.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>6-carboxyhexanoyl-[ACP] + H(+) + L-alanine = (8S)-8-amino-7-oxononanoate + CO2 + holo-[ACP]</text>
            <dbReference type="Rhea" id="RHEA:42288"/>
            <dbReference type="Rhea" id="RHEA-COMP:9685"/>
            <dbReference type="Rhea" id="RHEA-COMP:9955"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57972"/>
            <dbReference type="ChEBI" id="CHEBI:64479"/>
            <dbReference type="ChEBI" id="CHEBI:78846"/>
            <dbReference type="ChEBI" id="CHEBI:149468"/>
            <dbReference type="EC" id="2.3.1.47"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; biotin biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-II pyridoxal-phosphate-dependent aminotransferase family. BioF subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.47" evidence="1"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07001.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515035.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q1LS75"/>
    <dbReference type="SMR" id="Q1LS75"/>
    <dbReference type="STRING" id="266264.Rmet_0115"/>
    <dbReference type="EnsemblBacteria" id="ABF07001">
        <property type="protein sequence ID" value="ABF07001"/>
        <property type="gene ID" value="Rmet_0115"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0115"/>
    <dbReference type="eggNOG" id="COG0156">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015846_11_2_4"/>
    <dbReference type="OMA" id="HYHASGI"/>
    <dbReference type="OrthoDB" id="479874at2"/>
    <dbReference type="UniPathway" id="UPA00078"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008710">
        <property type="term" value="F:8-amino-7-oxononanoate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009102">
        <property type="term" value="P:biotin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01693">
        <property type="entry name" value="BioF_aminotrans_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004723">
        <property type="entry name" value="AONS_Archaea/Proteobacteria"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022834">
        <property type="entry name" value="AONS_Proteobacteria"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="SSF53383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00858">
        <property type="entry name" value="bioF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0093">Biotin biosynthesis</keyword>
    <keyword id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000381088" description="8-amino-7-oxononanoate synthase">
        <location>
            <begin position="1"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="20"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="116"/>
            <end position="117"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="141"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="187"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="215"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="243"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="366"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="1">
        <location>
            <position position="246"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01693"/>
        </source>
    </evidence>
    <sequence length="406" mass="42228" checksum="1F11AC40F06A019C" modified="2006-05-30" version="1">MLLDHLKRAAAERDAQSLTRRRRIAHTACAPHQSVGGIDATPTPLLTFCSNDYLGLANHGSIAHALAEGAHRYGAGSGASHLVSGHSLAHAQLERELARWLAPYIPQAQALYFCTGYMANLAVLTSLGATGATLFCESLNHASLIDGARLARADVQRYPHGDAGALDALLAASTTALKLIVTDSVFSMDGDIAPLAALLEVAERHDAWIIVDDAHGFGVLGENGHGVLEHLGLRSERLIYIGTLGKAAGVAGAFVAAHETIVEHLINTARPYIYTTAAPPAVAHALLTSLEIIAGEEGRSRRAHLTGLIAQLRAGLAVLAAQAGWSLGESETAIQPLIVGDNAAALALSAALEAEGIRVGAIRPPTVPAGTARLRITLSASHSAADVARLLETIARAAPQLHKEAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LS99</accession>
    <name>Q1LS99_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Response regulator without effector domain</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_0091</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF06977.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0091"/>
    <dbReference type="EnsemblBacteria" id="ABF06977">
        <property type="protein sequence ID" value="ABF06977"/>
        <property type="gene ID" value="Rmet_0091"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0091"/>
    <dbReference type="eggNOG" id="COG0745">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_69_15_4"/>
    <dbReference type="OMA" id="VYTNHAQ"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Response regulatory" evidence="2">
        <location>
            <begin position="14"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="1">
        <location>
            <position position="65"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF06977.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="135" mass="15217" checksum="BCFCA7436528F5C4" modified="2006-05-30" version="1">MPEQPTASIPRPLRVLLIEDSAVIRSMLMEYLTAFPFVEAVDWADTESKAVGLAAAGAYHVAIVDLQLRQGNGINVLREMQKARFPGIRIVYTNHAQVSMYRRQCAEAGADYFFDKSLEMEQVFRVIEDYAMSTP</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-01-15" modified="2022-05-25" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LSE0</accession>
    <name>GATC_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase subunit C</fullName>
            <shortName evidence="1">Asp/Glu-ADT subunit C</shortName>
            <ecNumber evidence="1">6.3.5.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">gatC</name>
        <name type="ordered locus">Rmet_0050</name>
    </gene>
    <organism>
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp-tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases. The reaction takes place in the presence of glutamine and ATP through an activated phospho-Asp-tRNA(Asn) or phospho-Glu-tRNA(Gln).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + H2O + L-glutamine + L-glutamyl-tRNA(Gln) = ADP + H(+) + L-glutamate + L-glutaminyl-tRNA(Gln) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:17521"/>
            <dbReference type="Rhea" id="RHEA-COMP:9681"/>
            <dbReference type="Rhea" id="RHEA-COMP:9684"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:78520"/>
            <dbReference type="ChEBI" id="CHEBI:78521"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + H2O + L-aspartyl-tRNA(Asn) + L-glutamine = ADP + 2 H(+) + L-asparaginyl-tRNA(Asn) + L-glutamate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:14513"/>
            <dbReference type="Rhea" id="RHEA-COMP:9674"/>
            <dbReference type="Rhea" id="RHEA-COMP:9677"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:78515"/>
            <dbReference type="ChEBI" id="CHEBI:78516"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Heterotrimer of A, B and C subunits.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the GatC family.</text>
    </comment>
    <dbReference type="EC" id="6.3.5.-" evidence="1"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF06936.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008648047.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q1LSE0"/>
    <dbReference type="SMR" id="Q1LSE0"/>
    <dbReference type="STRING" id="266264.Rmet_0050"/>
    <dbReference type="EnsemblBacteria" id="ABF06936">
        <property type="protein sequence ID" value="ABF06936"/>
        <property type="gene ID" value="Rmet_0050"/>
    </dbReference>
    <dbReference type="GeneID" id="60822769"/>
    <dbReference type="KEGG" id="rme:Rmet_0050"/>
    <dbReference type="eggNOG" id="COG0721">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_105899_2_2_4"/>
    <dbReference type="OMA" id="VTPMAMK"/>
    <dbReference type="OrthoDB" id="1780195at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050566">
        <property type="term" value="F:asparaginyl-tRNA synthase (glutamine-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050567">
        <property type="term" value="F:glutaminyl-tRNA synthase (glutamine-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006450">
        <property type="term" value="P:regulation of translational fidelity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00122">
        <property type="entry name" value="GatC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003837">
        <property type="entry name" value="Asp/Glu-ADT_csu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036113">
        <property type="entry name" value="Asp/Glu-ADT_sf_sub_c"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15004">
        <property type="entry name" value="PTHR15004"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02686">
        <property type="entry name" value="Glu-tRNAGln"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF141000">
        <property type="entry name" value="SSF141000"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00135">
        <property type="entry name" value="gatC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_1000016187" description="Aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase subunit C">
        <location>
            <begin position="1"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00122"/>
        </source>
    </evidence>
    <sequence length="99" mass="10972" checksum="3C8B49E7EB8B37A2" modified="2006-05-30" version="1">MALALSDVKRIAHLARIETSDAEAEQTLAQLNNFFSLVEQMQAVDTTGIEPLAHPLSAVRDIAQRLREDAVTESDRRADYQRPAPATENGLYLVPKVIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="104" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LSG3</accession>
    <name>Q1LSG3_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Response regulator, OmpR-family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Rmet_0027</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF06913.1"/>
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    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_007973.1"/>
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    <dbReference type="STRING" id="266264.Rmet_0027"/>
    <dbReference type="EnsemblBacteria" id="ABF06913">
        <property type="protein sequence ID" value="ABF06913"/>
        <property type="gene ID" value="Rmet_0027"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0027"/>
    <dbReference type="eggNOG" id="COG0745">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_30_0_4"/>
    <dbReference type="OMA" id="TKLALWP"/>
    <dbReference type="OrthoDB" id="817710at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00383">
        <property type="entry name" value="trans_reg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001867">
        <property type="entry name" value="OmpR/PhoB-type_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016032">
        <property type="entry name" value="Sig_transdc_resp-reg_C-effctor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039420">
        <property type="entry name" value="WalR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48111">
        <property type="entry name" value="PTHR48111"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00486">
        <property type="entry name" value="Trans_reg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00862">
        <property type="entry name" value="Trans_reg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46894">
        <property type="entry name" value="SSF46894"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51755">
        <property type="entry name" value="OMPR_PHOB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Response regulatory" evidence="4">
        <location>
            <begin position="2"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="domain" description="OmpR/PhoB-type" evidence="5">
        <location>
            <begin position="139"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="OmpR/PhoB-type" evidence="3">
        <location>
            <begin position="139"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="2">
        <location>
            <position position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABF06913.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="255" mass="27947" checksum="0A84FC6E0407521B" modified="2006-05-30" version="1">MRIAALEDDPARAIVLERALHPGGHTLARFRSSQPMIEAMRREPFDLLLLDRDVAGTNADDLLDWVRRTLGHGMPVMMLGQSDAEEVVVGCLAAGADAYVPQPARCDELRARIEALTRRAAVPGMTVATVPPGALGDTSDQLVCGVFRFAVAEHRVWVNSMTVALSPKEYALALLLFRNLGALVTRRAMIEHVWPATGIKEQARTIDSHLSRVRTKLALWPFNGIMLRSVYRLGSRLDPIETAVATRGRPSLAAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-01-04" modified="2021-06-02" version="99" xmlns="http://uniprot.org/uniprot">
    <accession>Q5NUZ3</accession>
    <name>Q5NUZ3_CUPMC</name>
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        <submittedName>
            <fullName evidence="7">Tyrosine-based site-specific recombinase BimA</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">bimA1</name>
        <name evidence="7" type="ordered locus">Rmet_6193</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="7 8">
        <name>pMOL28</name>
    </geneLocation>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="8">Plasmid pMOL28</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000355">
        <property type="protein sequence ID" value="ABF13052.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011239980.1">
        <property type="nucleotide sequence ID" value="NC_007972.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_161719.1">
        <property type="nucleotide sequence ID" value="NC_006525.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF13052">
        <property type="protein sequence ID" value="ABF13052"/>
        <property type="gene ID" value="Rmet_6193"/>
    </dbReference>
    <dbReference type="GeneID" id="60825790"/>
    <dbReference type="KEGG" id="rme:Rmet_6193"/>
    <dbReference type="HOGENOM" id="CLU_556302_0_0_4"/>
    <dbReference type="OrthoDB" id="745068at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid pMOL28"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.443.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044068">
        <property type="entry name" value="CB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011010">
        <property type="entry name" value="DNA_brk_join_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013762">
        <property type="entry name" value="Integrase-like_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002104">
        <property type="entry name" value="Integrase_catalytic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010998">
        <property type="entry name" value="Integrase_recombinase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004107">
        <property type="entry name" value="Integrase_SAM-like_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02899">
        <property type="entry name" value="Phage_int_SAM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00589">
        <property type="entry name" value="Phage_integrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56349">
        <property type="entry name" value="SSF56349"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51900">
        <property type="entry name" value="CB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51898">
        <property type="entry name" value="TYR_RECOMBINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0229">DNA integration</keyword>
    <keyword evidence="3" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="2 4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-0614">Plasmid</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Core-binding (CB)" evidence="6">
        <location>
            <begin position="164"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="domain" description="Tyr recombinase" evidence="5">
        <location>
            <begin position="283"/>
            <end position="487"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABF13052.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="495" mass="53556" checksum="FE86B1427E827C14" modified="2005-01-04" version="1">MNPADWIDTGAVPPRPLPAKVDAALAYLAEALGHPVYAHWTLTRIKRRYGSLADAKAAQPTVLKLLLTHDGAVEYWERGRLRTAPADQAPSPDAVLARLLHTHRRRFRSADASANAGAVPATAQTTGLVANPWLAAHSHADHAWLARTGRFAQPQAAANTLGAVDDAQALALFLRDRTGRSSHTLRAYGAELRRLMRWCGTHGFGPFSDLTRQQLLAYRHTLEHGSSGTANTTPPLSEATRTRALAVVASLYGYWYATGYLHANPAAGLSAGSRARSGFVPTRLIPSALLDACDAWLDANSAGDLLPALRQRAIWALYRYAGVRLAELAWSAETALPRLEAEAPGRWTLYVCGKGRKVRAIPLPAQCMVVLRAYRRARGLPPEPSAHETLPVIHGSKGEALQQTGLYREIKAIFAVVADGLQAREPAKAMLLRAASPHWLRHAYARTLVVDHQVPLPAAQALLGHASVQTTAAYAKTDLTQLRAFVDATFADDVP</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-01-04" modified="2022-08-03" version="105" xmlns="http://uniprot.org/uniprot">
    <accession>Q5NV14</accession>
    <name>Q5NV14_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DNA helicase</fullName>
            <ecNumber evidence="2">3.6.4.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">uvrD2</name>
        <name evidence="6" type="ordered locus">Rmet_6248</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="6 7">
        <name>pMOL28</name>
    </geneLocation>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="7">Plasmid pMOL28</plasmid>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + H2O = ADP + H(+) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.12"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="3.6.4.12" evidence="2"/>
    <dbReference type="EMBL" id="CP000355">
        <property type="protein sequence ID" value="ABF13107.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011239937.1">
        <property type="nucleotide sequence ID" value="NC_007972.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_161676.1">
        <property type="nucleotide sequence ID" value="NC_006525.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF13107">
        <property type="protein sequence ID" value="ABF13107"/>
        <property type="gene ID" value="Rmet_6248"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6248"/>
    <dbReference type="HOGENOM" id="CLU_023291_2_0_4"/>
    <dbReference type="OMA" id="IRAPECA"/>
    <dbReference type="OrthoDB" id="137860at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid pMOL28"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003678">
        <property type="term" value="F:DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014017">
        <property type="entry name" value="DNA_helicase_UvrD-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000212">
        <property type="entry name" value="DNA_helicase_UvrD/REP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11070">
        <property type="entry name" value="PTHR11070"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13361">
        <property type="entry name" value="UvrD_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0347">Helicase</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0614">Plasmid</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UvrD-like helicase C-terminal" evidence="5">
        <location>
            <begin position="417"/>
            <end position="473"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001665"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF13361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABF13107.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="486" mass="54209" checksum="681253A476E03E0B" modified="2005-01-04" version="1">MTPTTEQEQVVETVRGGGPVKVKAYAGAGKTSTLRMAAEARGRARGLYLAFNKEIANEAARKFPQNTRCRTVHSLAYSGTRPEITRKLRNPVEPPHQLALRYGLGKLHLPTTIGKDLELSPSKVARMVMDGAARFCRSAQAEPLAWHIPVDAIVKDDEAEHLREALLPSVKRLWSEYLDPAAPSAIGHDTYVKLWQLSRPRIGADFILFDEAQDADGLMLSVLRAQQCQVIYVGDPYQQIYEWRGAVNAMDHIRAPECALTESFRFGPAIAQLASRVLALMDEDTPVRGQNGVASRILHESALGQERFDAVLCRKNATVLTHLAQGIGRGDRVAVRANVDELRAFADGAEQLMRGQRIGYPATLALFETWEEVQEYAESFAGRDLKPLVTLIDNEGVDYLRLILTRVSPEDEADYIVSTVHRAKGLEWDRVQLAGDFKFKNGDDGKLTMAPEEMRLLYVAMTRAKHVLDVSEIRRDLYTMFREAGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-03-23" modified="2020-10-07" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>D3DXY3</accession>
    <name>D3DXY3_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_6546</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ADC45153.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_6546"/>
    <dbReference type="EnsemblBacteria" id="ADC45153">
        <property type="protein sequence ID" value="ADC45153"/>
        <property type="gene ID" value="Rmet_6546"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6546"/>
    <dbReference type="HOGENOM" id="CLU_3047197_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADC45153.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="54" mass="6093" checksum="D56F677970F76B97" modified="2010-03-23" version="1">MTSSDFVVTLYSFGLEGNPTKLGSLCLHVIPYVYLLLFQETPDGTASWRYRTGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-03-23" modified="2020-10-07" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>D3DY93</accession>
    <name>D3DY93_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_6672</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ADC45263.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADC45263">
        <property type="protein sequence ID" value="ADC45263"/>
        <property type="gene ID" value="Rmet_6672"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6672"/>
    <dbReference type="HOGENOM" id="CLU_1957741_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADC45263.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="128" mass="14789" checksum="4F16CF74E97BB35A" modified="2010-03-23" version="1">MRATHYISGYTVQIYSIAMPRFPIGTDGRYNLPKLTAADLRALYFQCPEPETRALLWDIHRLRILVVHVDQLQRTMASHCHISTLQVLESLRAALAGEPCVLEHAARIEETFEPHSAREYTRKCNRRD</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1994-10-01" modified="2022-05-25" version="98" xmlns="http://uniprot.org/uniprot">
    <accession>P37974</accession>
    <accession>Q5NUX3</accession>
    <accession>Q7B056</accession>
    <name>CNRC_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName>Nickel and cobalt resistance protein CnrC</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">cnrC</name>
        <name type="ordered locus">Rmet_6208</name>
        <name type="ORF">RMe0085</name>
    </gene>
    <organism>
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid">
        <name>pMOL28</name>
    </geneLocation>
    <reference key="1">
        <citation type="journal article" date="1993" name="J. Bacteriol." volume="175" first="767" last="778">
            <title>Characterization of the inducible nickel and cobalt resistance determinant cnr from pMOL28 of Alcaligenes eutrophus CH34.</title>
            <authorList>
                <person name="Liesegang H."/>
                <person name="Lemke K."/>
                <person name="Siddiqui R.A."/>
                <person name="Schlegel H.G."/>
            </authorList>
            <dbReference type="PubMed" id="8380802"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC DNA]</scope>
    </reference>
    <reference key="2">
        <citation type="submission" date="2004-10" db="EMBL/GenBank/DDBJ databases">
            <title>Sequence and features of the Ralstonia metallidurans CH34 heavy metal plasmids pMOL28 and pMOL30.</title>
            <authorList>
                <person name="van der Lelie D."/>
                <person name="Monchy S."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="Benotmane M."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Mergeay M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference key="3">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <reference key="4">
        <citation type="journal article" date="2000" name="J. Bacteriol." volume="182" first="1390" last="1398">
            <title>Regulation of the cnr cobalt and nickel resistance determinant from Ralstonia sp. strain CH34.</title>
            <authorList>
                <person name="Grass G."/>
                <person name="Grosse C."/>
                <person name="Nies D.H."/>
            </authorList>
            <dbReference type="PubMed" id="10671463"/>
            <dbReference type="DOI" id="10.1128/JB.182.5.1390-1398.2000"/>
        </citation>
        <scope>INDUCTION</scope>
    </reference>
    <comment type="function">
        <text>The products of the genes cnrA, cnrB, and cnrC are likely to form a membrane-bound protein complex catalyzing an energy-dependent efflux of Ni(2+) and Co(2+). The mechanism of action of the CnrCBA complex may be that of a proton/cation antiporter.</text>
    </comment>
    <comment type="induction">
        <text evidence="2">By nickel.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the outer membrane factor (OMF) (TC 1.B.17) family.</text>
    </comment>
    <dbReference type="EMBL" id="M91650">
        <property type="protein sequence ID" value="AAA21968.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AJ276513">
        <property type="protein sequence ID" value="CAB82451.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="X90708">
        <property type="protein sequence ID" value="CAI30229.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP000355">
        <property type="protein sequence ID" value="ABF13067.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E47056">
        <property type="entry name" value="E47056"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011239968.1">
        <property type="nucleotide sequence ID" value="NC_007972.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_161707.1">
        <property type="nucleotide sequence ID" value="NC_006525.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="P37974"/>
    <dbReference type="SMR" id="P37974"/>
    <dbReference type="TCDB" id="1.B.17.2.1">
        <property type="family name" value="the outer membrane factor (omf) family"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF13067">
        <property type="protein sequence ID" value="ABF13067"/>
        <property type="gene ID" value="Rmet_6208"/>
    </dbReference>
    <dbReference type="GeneID" id="60825774"/>
    <dbReference type="KEGG" id="rme:Rmet_6208"/>
    <dbReference type="HOGENOM" id="CLU_012817_14_2_4"/>
    <dbReference type="OMA" id="HIEIQRL"/>
    <dbReference type="OrthoDB" id="1533572at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid pMOL28"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015562">
        <property type="term" value="F:efflux transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003423">
        <property type="entry name" value="OMP_efflux"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02321">
        <property type="entry name" value="OEP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword id="KW-0170">Cobalt</keyword>
    <keyword id="KW-0533">Nickel</keyword>
    <keyword id="KW-0614">Plasmid</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0732">Signal</keyword>
    <keyword id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000020952" description="Nickel and cobalt resistance protein CnrC">
        <location>
            <begin position="30"/>
            <end position="418"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000269" key="2">
        <source>
            <dbReference type="PubMed" id="10671463"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="418" mass="43760" checksum="C7A277D975CE69E5" modified="1995-02-01" version="2" precursor="true">MKQVISSFLCRPRFVGSAIWLLPVALSHAAEAPPFPNLLQQSLALAPAMVAQAANVRAAGADAAQAQAWLNPRIDTVLENLGAPSSDGLSQRQNTYSITQPFELGGKRGARIEVGERNFAAAQARERQAQVAYAAELAVAYATAEAALGRKILATENLARANEELAAARALVDSGKEASLRSAQAKASVAAAQAAEAAATNDATQALARLSAMSGASEPYTAVTSSLLTTQAVVPNAPAALAESPSVRAAEAERNALDAQVDVERKRWIPDVGVSAGVRRYGWTNSSGYVVGVTASIPLFDQNRNGINAAVERVAAAQARLDSVRLEANVARQSAISQVATADKQLAAASEGEQAAAEAYRMGRIGYESGKTPLMELLAVRRALVDARQLTIDARLARVRALAALAQADGRLAFEESR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-10-07" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>Q1L9P8</accession>
    <name>Q1L9P8_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_6269</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2 3">
        <name>pMOL28</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid pMOL28</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000355">
        <property type="protein sequence ID" value="ABF13128.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF13128">
        <property type="protein sequence ID" value="ABF13128"/>
        <property type="gene ID" value="Rmet_6269"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6269"/>
    <dbReference type="HOGENOM" id="CLU_1053224_0_0_4"/>
    <dbReference type="OMA" id="GEIHNSD"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid pMOL28"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="238"/>
            <end position="262"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF13128.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="264" mass="29932" checksum="AF75396A46FE6D9F" modified="2006-05-30" version="1">MLHGLTGGTNRQYKALVEDAIENAPGELVFGETKFAKLYRGIQVEMDDWTEIPLRDAFRMQFKAALTPMRMFMVAAGMRRERRTMADRFGANGFRRLQDIGGSAAFHALSPDERRRIAGFPEPYAYLVENCRRRAGGGDRCGPVFPDPDWRWLAMIEPELNIPLRSVFMLEFAVERARVFGGSEISIFVGEIHNSDMVWLAGFDESAVDPKVRDMVDNVRWRAIELARAPRRPSRLRFVLASLAGASIPLSLYFGLGTWLWARG</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-10-07" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>Q1L9R6</accession>
    <name>Q1L9R6_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_6251</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2 3">
        <name>pMOL28</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid pMOL28</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000355">
        <property type="protein sequence ID" value="ABF13110.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF13110">
        <property type="protein sequence ID" value="ABF13110"/>
        <property type="gene ID" value="Rmet_6251"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6251"/>
    <dbReference type="HOGENOM" id="CLU_2234310_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid pMOL28"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="84"/>
            <end position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF13110.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="105" mass="11383" checksum="0A9AF798D77E1C48" modified="2006-05-30" version="1">MEKHTVRENIPSGAESPSLVSIGRRPIAARAKGGYLVHLVDLATGLALCGHKPRHTAKRMKRRSGWLPYGAVPPTCIKCEAAQPPATEAPERDPLTIDMFEDAQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="59" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LA01</accession>
    <name>Q1LA01_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Type-F conjugative transfer system pilin assembly protein TrbC</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">trbC</name>
        <name evidence="1" type="ordered locus">Rmet_6166</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="1 2">
        <name>pMOL30</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid pMOL30</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000354">
        <property type="protein sequence ID" value="ABF13025.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011514826.1">
        <property type="nucleotide sequence ID" value="NC_006466.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF13025">
        <property type="protein sequence ID" value="ABF13025"/>
        <property type="gene ID" value="Rmet_6166"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6166"/>
    <dbReference type="HOGENOM" id="CLU_091617_0_0_4"/>
    <dbReference type="OMA" id="VMVVRGF"/>
    <dbReference type="OrthoDB" id="1761021at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid pMOL30"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019106">
        <property type="entry name" value="T4SS_TrbC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014113">
        <property type="entry name" value="T4SS_TrbC_subgr"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09673">
        <property type="entry name" value="TrbC_Ftype"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02742">
        <property type="entry name" value="TrbC_Ftype"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF13025.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="205" mass="21881" checksum="111B5E2997D50464" modified="2006-05-30" version="1">MAKHREEAAAVLGQNIKPVKPGTFKVEVPTIKAPPPVRTQSLDDIMQQYAEAKKGGPAPAKQGANDLIIFVSFSMPKPVLTELARQAKETGAVMVVRGFKDGSLKATKLAALEVNRAGAPWEIHPDLFKAFKVTAVPTFVVASAEAESVLDDGCSPETAYSAVAGNMSVELALDTIRRRAQPTIAKLAENRLAAIRAKNQPGTLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="58" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LAB2</accession>
    <name>Q1LAB2_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_6055</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2 3">
        <name>pMOL30</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid pMOL30</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000354">
        <property type="protein sequence ID" value="ABF12914.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011514777.1">
        <property type="nucleotide sequence ID" value="NC_007971.2"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF12914">
        <property type="protein sequence ID" value="ABF12914"/>
        <property type="gene ID" value="Rmet_6055"/>
    </dbReference>
    <dbReference type="GeneID" id="60825077"/>
    <dbReference type="KEGG" id="rme:Rmet_6055"/>
    <dbReference type="HOGENOM" id="CLU_709539_0_0_4"/>
    <dbReference type="OMA" id="SSYIYGR"/>
    <dbReference type="OrthoDB" id="1476147at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid pMOL30"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="20"/>
            <end position="40"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF12914.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="381" mass="42765" checksum="9ECC72DE3F7B411D" modified="2006-05-30" version="1">MVTEEVAEFIPAEDEGVDPVDTTEVDGFDTPTATGKPSELERMSAEFLDNPKRPKTFIDRFIKAMYADERTHKICRVRALKSKVDLGDVDEILQRVVVVFFATKQLDKLREANAIYAVIYAIASNVCREHIRDALALTINHDSIEEMRERGEELENVGLVDSEPIDRDQVIDSRTAAAKMALAFRRHLNGEEKLSNLGVFDLDPLIAPVAPAPESAAADKNLEALPAPAAPKPRVRRANRNETLSADQLELVQICADLQMRNQDFAAALGIGLPRLSSYIYGRTASVPADIMTAARKLRDEEPEAMARREMFNRPMSDILGRWANDLQVESDTDLATILGVTKMTIFRWRTDETKPDNTALNRYEQVVRTFKNRLAAIKQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="55" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LAY4</accession>
    <name>Q1LAY4_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_5833</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12692.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518042.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF12692">
        <property type="protein sequence ID" value="ABF12692"/>
        <property type="gene ID" value="Rmet_5833"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5833"/>
    <dbReference type="HOGENOM" id="CLU_2411058_0_0_4"/>
    <dbReference type="OMA" id="RGKWACC"/>
    <dbReference type="OrthoDB" id="1991100at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF12692.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="92" mass="10025" checksum="98759E48D3C03831" modified="2006-05-30" version="1">MDRSLDILAVHGFAVREGRGKWACCYEIRLAIVGGPLLYRGELHGRNFATEEAAIIAAVEIGEREAGRHIDAARAMIVALARITDGAHHEMS</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="72" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LAY9</accession>
    <name>Q1LAY9_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Isochorismatase hydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">ycaC</name>
        <name evidence="2" type="ordered locus">Rmet_5828</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12687.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008646397.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5828"/>
    <dbReference type="EnsemblBacteria" id="ABF12687">
        <property type="protein sequence ID" value="ABF12687"/>
        <property type="gene ID" value="Rmet_5828"/>
    </dbReference>
    <dbReference type="GeneID" id="60823573"/>
    <dbReference type="KEGG" id="rme:Rmet_5828"/>
    <dbReference type="eggNOG" id="COG1335">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_066901_1_2_4"/>
    <dbReference type="OMA" id="CVAMPAI"/>
    <dbReference type="OrthoDB" id="1860541at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.850">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000868">
        <property type="entry name" value="Isochorismatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036380">
        <property type="entry name" value="Isochorismatase-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00857">
        <property type="entry name" value="Isochorismatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52499">
        <property type="entry name" value="SSF52499"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Isochorismatase" evidence="1">
        <location>
            <begin position="19"/>
            <end position="172"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF12687.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="225" mass="24227" checksum="36448988727AD0B2" modified="2006-05-30" version="1">MTQPANFNGARPVIDPADTAMLLIDHQSGLFQTVGDMPMPELRQRAAALAKMATLSKLPVITTASVPQGPNGPLIPEIHENAPHAKYVARKGEINAWDNPEFVAAVKATGKKTLIIAGTITSVCMAFPSISAVADGYKVFAVVDASGTYSKMAQEITLARVVQAGVVPMDTAAVASELQRTWNRDDAQQWAEIYTKIFPAYQLLIESYLKAQDVQKNGEALDSSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="106" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LAZ9</accession>
    <name>Q1LAZ9_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Cold-shock DNA-binding protein family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">csp</name>
        <name evidence="4" type="ordered locus">Rmet_5818</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="5">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="5">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12677.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_006159056.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5818"/>
    <dbReference type="EnsemblBacteria" id="ABF12677">
        <property type="protein sequence ID" value="ABF12677"/>
        <property type="gene ID" value="Rmet_5818"/>
    </dbReference>
    <dbReference type="GeneID" id="60823562"/>
    <dbReference type="KEGG" id="rme:Rmet_5818"/>
    <dbReference type="eggNOG" id="COG1278">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_117621_0_3_4"/>
    <dbReference type="OMA" id="IMGLVKW"/>
    <dbReference type="OrthoDB" id="2034748at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04458">
        <property type="entry name" value="CSP_CDS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012156">
        <property type="entry name" value="Cold_shock_CspA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011129">
        <property type="entry name" value="CSD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019844">
        <property type="entry name" value="CSD_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002059">
        <property type="entry name" value="CSP_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00313">
        <property type="entry name" value="CSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002599">
        <property type="entry name" value="Cold_shock_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00050">
        <property type="entry name" value="COLDSHOCK"/>
    </dbReference>
    <dbReference type="SMART" id="SM00357">
        <property type="entry name" value="CSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00352">
        <property type="entry name" value="CSD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51857">
        <property type="entry name" value="CSD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0614">Plasmid</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CSD" evidence="3">
        <location>
            <begin position="1"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU000408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF12677.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="67" mass="7163" checksum="73687BC8234B26EB" modified="2006-05-30" version="1">MATGTVKWFNETKGFGFITPDDGGADLFAHFSEIQGSGFKTLKDGQRVTFEVKQGPKGLQASAIKPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-10-07" version="56" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LB97</accession>
    <name>Q1LB97_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_5720</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12579.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5720"/>
    <dbReference type="EnsemblBacteria" id="ABF12579">
        <property type="protein sequence ID" value="ABF12579"/>
        <property type="gene ID" value="Rmet_5720"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5720"/>
    <dbReference type="eggNOG" id="COG1404">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1685141_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193262" evidence="1">
        <location>
            <begin position="46"/>
            <end position="156"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF12579.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="156" mass="16457" checksum="5CA688924182F652" modified="2006-05-30" version="1">MPTAAPESEFGPPRAWVARKRWSLAMAMALPLGAVMLGAAPPAHAQKRNPSEVPSAPVVQLIVKESGAIRSAAAINGSGDEMRRVQQWSAAALQPLTSKRVMSGGARILRLSTPLTASDAAAVAQRLRATGAFDYVDVDYRIHVAGVAANDTYLLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LBA6</accession>
    <name>Q1LBA6_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_5711</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12570.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5711"/>
    <dbReference type="EnsemblBacteria" id="ABF12570">
        <property type="protein sequence ID" value="ABF12570"/>
        <property type="gene ID" value="Rmet_5711"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5711"/>
    <dbReference type="eggNOG" id="COG0457">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010140_2_0_4"/>
    <dbReference type="OMA" id="KVGLAWT"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_repeat"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="SSF48452"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="1">
        <location>
            <begin position="114"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="1">
        <location>
            <begin position="148"/>
            <end position="181"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF12570.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="488" mass="52769" checksum="735AA9320A901796" modified="2006-05-30" version="1">MIRDWRKRTPESVDAIVAQAALDVELGDTASARTALEQVLATVPNHPRALAGLAHLALCAGDAKSAVDWFQQTGPEALDEAELSDWCEALTATGDLTHGADIARHLCERAPKAATSWFQLGLTLHHSGFHEQALDAYPKAHALDPDLDNLCNNIGAAYLQSGRTEEAMTPIRRAIELKPSNPLAWINLSDALLKLRRPDDAALAARRACALAPTMPLALQAQSNALKDLGQWADALALMEKAAMQAPVSGGIIWNLAMLQMLHGDYRNGLINFEHRWASPELAGTFPDLKAPIRRGESLAGKTLFLWGEQGFGDAMQFVRFVPGIAEFVRQQGGQLYYCCFSKLHTLFSRGLADVTPEVIPHDTPSLPRFGYHLPLCSAPLRLGTELDTLPADVPYLRADAAAASAWAQRLSASGKLNVGVVWSGSRGHQRNPLRAVPVAELAGALSDLPGIEFHSMQIDGGRNWQASPISISVITLRNCEATTTPPH</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="66" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LBF3</accession>
    <name>Q1LBF3_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_5664</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12523.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011520069.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5664"/>
    <dbReference type="EnsemblBacteria" id="ABF12523">
        <property type="protein sequence ID" value="ABF12523"/>
        <property type="gene ID" value="Rmet_5664"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5664"/>
    <dbReference type="eggNOG" id="ENOG50333YQ">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_142719_0_0_4"/>
    <dbReference type="OMA" id="WANDSTK"/>
    <dbReference type="OrthoDB" id="1916766at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022236">
        <property type="entry name" value="DUF3761"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12587">
        <property type="entry name" value="DUF3761"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193282" evidence="2">
        <location>
            <begin position="23"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="89"/>
            <end position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF12523.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="165" mass="15900" checksum="F234262A5526DC32" modified="2006-05-30" version="1">MKNLLKSMAVAAALGLSLGAQAQAPAGAPAGSTALCKDGSYYSGASKKGACAGHKGVKDWYGGAAAASAPAAMAPAAMAPAAAPAPKAAAPAATAAPAAPATATEKKTTAAAPGGGPGQVWANSSTKVYHCPSDRYYGKTKSGAYMSEADAKAKGYKPDHGKACS</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LBH5</accession>
    <name>Q1LBH5_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Flagellar hook-associated protein 2</fullName>
            <shortName evidence="5">HAP2</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Flagellar cap protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">fliD3</name>
        <name evidence="8" type="ordered locus">Rmet_5642</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="9">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="9">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Required for morphogenesis and for the elongation of the flagellar filament by facilitating polymerization of the flagellin monomers at the tip of growing filament. Forms a capping structure, which prevents flagellin subunits (transported through the central channel of the flagellum) from leaking out without polymerization at the distal end.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2 5">Homopentamer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Secreted</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Bacterial flagellum</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the FliD family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12501.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011520046.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5642"/>
    <dbReference type="EnsemblBacteria" id="ABF12501">
        <property type="protein sequence ID" value="ABF12501"/>
        <property type="gene ID" value="Rmet_5642"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5642"/>
    <dbReference type="eggNOG" id="COG1345">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015182_4_1_4"/>
    <dbReference type="OMA" id="DWGMTER"/>
    <dbReference type="OrthoDB" id="883503at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009421">
        <property type="term" value="C:bacterial-type flagellum filament cap"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009424">
        <property type="term" value="C:bacterial-type flagellum hook"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007155">
        <property type="term" value="P:cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010810">
        <property type="entry name" value="Flagellin_hook_IN_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040026">
        <property type="entry name" value="FliD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010809">
        <property type="entry name" value="FliD_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003481">
        <property type="entry name" value="FliD_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30288">
        <property type="entry name" value="PTHR30288"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07196">
        <property type="entry name" value="Flagellin_IN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07195">
        <property type="entry name" value="FliD_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02465">
        <property type="entry name" value="FliD_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0975">Bacterial flagellum</keyword>
    <keyword evidence="8" id="KW-0966">Cell projection</keyword>
    <keyword evidence="8" id="KW-0969">Cilium</keyword>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8" id="KW-0282">Flagellum</keyword>
    <keyword evidence="8" id="KW-0614">Plasmid</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0964">Secreted</keyword>
    <feature type="domain" description="FliD_N" evidence="6">
        <location>
            <begin position="11"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="domain" description="FliD_C" evidence="7">
        <location>
            <begin position="246"/>
            <end position="468"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009764"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023143"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU362066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF02465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF07195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ABF12501.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="487" mass="50547" checksum="DDAEACDF78954AEE" modified="2006-05-30" version="1">MAALDFLGIGSNMQLGTLLDQLATAERLPLKALETQNTAYQAKLSGYGTIKSMLSAFQASANSLAKLDTFGAVKAQVSNSDVMTVATGVNAIAGNYNVNVKSLAQAQSLAGAGQLKQDAIIGTGTLTFEFGVTGGYDADPASPTYGTYVNPTFTPTADSAKTVTIDSSNQTLQGIRDAINKANMGVKASIINDGSGTPYRLVLNSEKTGEAMSMRITGSSPELQALAGYDPVAGPQPGGMKEVVRATNAQLTINDIAITSASNVVTDAAQGVTMTLKKTGTTSMTLTQDLDSIKAAVQGLATAYNNLQSATKQLTAFDVKSGTKAALTGDGVLRGIQSTLRSVLNTPEAGMRDGAATTLAQLGVTLQKDGTMSVDTKKLDAALAGNLADVTALFGGDGTKGGIARRLSEAVDTMTGTNGPLTSATKGIEQSVKDLGKRYDSMSEHIDANIKRVQKQFQALDLAVSQMNRTKDYLTQQFSAMNNTNSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LBI0</accession>
    <name>Q1LBI0_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 8 10">FAD:protein FMN transferase</fullName>
            <ecNumber evidence="2 8 10">2.7.1.180</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Flavin transferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">apbE</name>
        <name evidence="11" type="ordered locus">Rmet_5637</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="12">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="12">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Flavin transferase that catalyzes the transfer of the FMN moiety of FAD and its covalent binding to the hydroxyl group of a threonine residue in a target flavoprotein.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 8 10">
            <text>FAD + L-threonyl-[protein] = AMP + FMN-L-threonyl-[protein] + H(+)</text>
            <dbReference type="Rhea" id="RHEA:36847"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11061"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
            <dbReference type="ChEBI" id="CHEBI:74257"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="2.7.1.180"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <cofactor evidence="9">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="9">Magnesium. Can also use manganese.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Cell inner membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8 10">Belongs to the ApbE family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.180" evidence="2 8 10"/>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12496.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011520040.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5637"/>
    <dbReference type="EnsemblBacteria" id="ABF12496">
        <property type="protein sequence ID" value="ABF12496"/>
        <property type="gene ID" value="Rmet_5637"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5637"/>
    <dbReference type="eggNOG" id="COG1477">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_044403_0_2_4"/>
    <dbReference type="OMA" id="MGTFWRV"/>
    <dbReference type="OrthoDB" id="2021063at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017013">
        <property type="term" value="P:protein flavinylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.520.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024932">
        <property type="entry name" value="ApbE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003374">
        <property type="entry name" value="ApbE-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30040">
        <property type="entry name" value="PTHR30040"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02424">
        <property type="entry name" value="ApbE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006268">
        <property type="entry name" value="ApbE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143631">
        <property type="entry name" value="SSF143631"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="10" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8 10" id="KW-0274">FAD</keyword>
    <keyword evidence="4 8 10" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="10 11" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="7 8 9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 8 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0614">Plasmid</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8 10" id="KW-0808">Transferase</keyword>
    <feature type="binding site" evidence="9">
        <location>
            <position position="171"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="289"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="293"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001219"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011955"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00016337"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRNR" id="PIRNR006268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR006268-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU363002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ABF12496.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="337" mass="36101" checksum="9DA260FA22C841A8" modified="2006-05-30" version="1">MNRVLIPATLDLPPPVREPQGSIAQWTGETMGTTWAVRAVLARGQDGGGLEAGIRAVLDEVIAQMSNWSEISDLSRFNRADASAWVTLPEDCFTVLQAALDTARDSGGAYDPSAGPLVDLWGFGPAGKRHTAPTADDVARARQHCGWQRIEIDPSARRARQPGGLSLDFCAIAKGFAVDAVSRYLLAQGVPHHLVEIGGELRGQGLKPDGMPWWIELESPSASATAAGGIRRTLVALLGLSIATSGDYRRYFEHDGRRYAHTIDPRTGYPAAHALASVTVLHRECMMADALSTALTVLGPEAGITFATRQGLAARFLVRTPHGFDERVTPAFAAMQT</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LBL6</accession>
    <name>Q1LBL6_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Short-chain dehydrogenase/reductase SDR putative 3-oxoacyl-(Acyl-carrier-protein) reductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_5601</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12460.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011520008.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5601"/>
    <dbReference type="EnsemblBacteria" id="ABF12460">
        <property type="protein sequence ID" value="ABF12460"/>
        <property type="gene ID" value="Rmet_5601"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5601"/>
    <dbReference type="eggNOG" id="COG4221">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010194_2_10_4"/>
    <dbReference type="OMA" id="ANVQFMT"/>
    <dbReference type="OrthoDB" id="1261526at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00061">
        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU000363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF12460.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="252" mass="26256" checksum="09CF1198F923B51C" modified="2006-05-30" version="1">MTKQAKVALVTGAARGIGKAVAQAMLADGYQVVLAGRTAETLEALAAQATAAGQTALAVQCDVSDAASVDALFGKIRERFGRLDVLFNNAGINAPGVSIDELSVEQWKAVVDTNLTGAFLCARGAFALMKSQDPRGGRIINNGSISAHAPRPNSVAYTATKHAITGLTKSLSLDGRPWDIACGQIDIGNAGTDMANRMARGVPQANGEIRPEPLMDVAHVASSVLHMANLPLETNVQFMTVMATKMPFVGRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LBT3</accession>
    <name>Q1LBT3_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">2-nitropropane dioxygenase, NPD</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_5534</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12393.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519938.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5534"/>
    <dbReference type="EnsemblBacteria" id="ABF12393">
        <property type="protein sequence ID" value="ABF12393"/>
        <property type="gene ID" value="Rmet_5534"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5534"/>
    <dbReference type="eggNOG" id="COG2070">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038732_1_0_4"/>
    <dbReference type="OMA" id="THESFAH"/>
    <dbReference type="OrthoDB" id="275153at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051213">
        <property type="term" value="F:dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018580">
        <property type="term" value="F:nitronate monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04730">
        <property type="entry name" value="NPD_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004136">
        <property type="entry name" value="NMO"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03060">
        <property type="entry name" value="NMO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NMO" evidence="1">
        <location>
            <begin position="3"/>
            <end position="309"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03060"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF12393.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="323" mass="34068" checksum="8A97D2F23EEB57AC" modified="2006-05-30" version="1">MKTRITELLNIAYPIIQGGMQWVGRAELASAVSNAGGLGVLTGLTQPTPEALHREIGRCREMTDRPFGVNMTIFPTIAPPPYEEYIDAIIANGVGVVETAGNKALEYLPKLKDAGIKVIHKCVAVRHALTAERLGVDAISIDGFECAGHPGEEDIPGLVLIPLAASQLSVPVIASGGIADGRGMAAALALGAHGVNMGTRFCATQEAPIHENIKQRLLAASERDTNLIFRSLKNTARVLKNAVSDEVVAAERRPGGCGFEDIRHLVAGARGRTALQEGDPDGGIVTAGMVVGLIRDLPSCAELLERMVAECRESLLTASAFAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LBW7</accession>
    <name>Q1LBW7_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_5500</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12359.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519901.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5500"/>
    <dbReference type="EnsemblBacteria" id="ABF12359">
        <property type="protein sequence ID" value="ABF12359"/>
        <property type="gene ID" value="Rmet_5500"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5500"/>
    <dbReference type="eggNOG" id="COG1545">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_119412_1_0_4"/>
    <dbReference type="OMA" id="FFDYPYV"/>
    <dbReference type="OrthoDB" id="1756670at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002878">
        <property type="entry name" value="DUF35_OB-fold_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022002">
        <property type="entry name" value="DUF35_Znr_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12172">
        <property type="entry name" value="DUF35_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01796">
        <property type="entry name" value="OB_aCoA_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF35_N" evidence="2">
        <location>
            <begin position="24"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="domain" description="OB_aCoA_assoc" evidence="1">
        <location>
            <begin position="59"/>
            <end position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01796"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF12359.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="132" mass="14664" checksum="229DF4DAF49F94EA" modified="2006-05-30" version="1">MTTPHVDRPLAAPVVDASNESYWRYAREGMLSSRRCKSCKKLHWYPRALCPFCQGETEWECLSGNGTIYSVSVTRKAGPIPYAIAYVTLDEGPTMLTNIVDCDLDSLKIGQRVKACFKPSEGDSPIPMFTPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LD75</accession>
    <name>Q1LD75_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Acetolactate synthase, large subunit (TPP dependent)</fullName>
            <ecNumber evidence="6">2.2.1.6</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Rmet_5039</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="7">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="7">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the TPP enzyme family.</text>
    </comment>
    <dbReference type="EC" id="2.2.1.6" evidence="6"/>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11901.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5039"/>
    <dbReference type="EnsemblBacteria" id="ABF11901">
        <property type="protein sequence ID" value="ABF11901"/>
        <property type="gene ID" value="Rmet_5039"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5039"/>
    <dbReference type="eggNOG" id="COG0028">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013748_3_1_4"/>
    <dbReference type="OMA" id="MATCGEV"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003984">
        <property type="term" value="F:acetolactate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030976">
        <property type="term" value="F:thiamine pyrophosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044281">
        <property type="term" value="P:small molecule metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
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        <property type="entry name" value="DHS-like_NAD/FAD-binding_dom"/>
    </dbReference>
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        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012000">
        <property type="entry name" value="Thiamin_PyroP_enz_cen_dom"/>
    </dbReference>
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        <property type="entry name" value="Thiamin_PyroP_enz_TPP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000399">
        <property type="entry name" value="TPP-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045229">
        <property type="entry name" value="TPP_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011766">
        <property type="entry name" value="TPP_enzyme-bd_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18968">
        <property type="entry name" value="PTHR18968"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02775">
        <property type="entry name" value="TPP_enzyme_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00205">
        <property type="entry name" value="TPP_enzyme_M"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="TPP_enzyme_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52467">
        <property type="entry name" value="SSF52467"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="SSF52518"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00187">
        <property type="entry name" value="TPP_ENZYMES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0614">Plasmid</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="TPP_enzyme_N" evidence="5">
        <location>
            <begin position="51"/>
            <end position="220"/>
        </location>
    </feature>
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        <location>
            <begin position="244"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="domain" description="TPP_enzyme_C" evidence="4">
        <location>
            <begin position="456"/>
            <end position="594"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007812"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU362132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF02775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF02776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABF11901.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="611" mass="65316" checksum="C571D8822C2F7731" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LDV0</accession>
    <name>Q1LDV0_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Threonine and homoserine efflux system</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">rhtA</name>
        <name evidence="3" type="ordered locus">Rmet_4814</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11676.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519239.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF11676">
        <property type="protein sequence ID" value="ABF11676"/>
        <property type="gene ID" value="Rmet_4814"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4814"/>
    <dbReference type="eggNOG" id="COG5006">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_057295_0_1_4"/>
    <dbReference type="OMA" id="MWAAYIV"/>
    <dbReference type="OrthoDB" id="1515911at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000620">
        <property type="entry name" value="EamA_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00892">
        <property type="entry name" value="EamA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
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    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="44"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="75"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="98"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="123"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="149"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="179"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="207"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="238"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="264"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="domain" description="EamA" evidence="2">
        <location>
            <begin position="150"/>
            <end position="277"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF11676.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="288" mass="29749" checksum="783DF3342CDF419E" modified="2006-05-30" version="1">MPLSRIKQSDSLLAVLALIGSMASLCIGTSFAKSLFASIGAQGTTALRVSFAAVILICVWRPWRFPLSGKNARAIALYGAALGGTNLLFYMSLRTIPLGLAIAIEFTGPLAVAVLSSRRAIDFLWIGCAVTGLILLLPLGEAATHLDPVGIGFALAAGVGWALYIVFGQMAGNAHGGQATSLGMTMAALVALPFGLGHAGLEMFSPALLLSGLAVGILSSAIPYSLEMVALKRLPRRTFGILLSLEPAMGALAGLAFLNEQLTPIQWVAIAAIIVASIGCTATSKSKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LE28</accession>
    <name>Q1LE28_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Probable 5-dehydro-4-deoxyglucarate dehydratase</fullName>
            <ecNumber evidence="4">4.2.1.41</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">5-keto-4-deoxy-glucarate dehydratase</fullName>
            <shortName evidence="4">KDGDH</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">kdgD</name>
        <name evidence="6" type="ordered locus">Rmet_4736</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="7">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="7">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 4">
            <text>5-dehydro-4-deoxy-D-glucarate + H(+) = 2,5-dioxopentanoate + CO2 + H2O</text>
            <dbReference type="Rhea" id="RHEA:24608"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:42819"/>
            <dbReference type="ChEBI" id="CHEBI:58136"/>
            <dbReference type="EC" id="4.2.1.41"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 4">Carbohydrate acid metabolism; D-glucarate degradation; 2,5-dioxopentanoate from D-glucarate: step 2/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 4 5">Belongs to the DapA family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.41" evidence="4"/>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11598.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519170.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4736"/>
    <dbReference type="EnsemblBacteria" id="ABF11598">
        <property type="protein sequence ID" value="ABF11598"/>
        <property type="gene ID" value="Rmet_4736"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4736"/>
    <dbReference type="eggNOG" id="COG0329">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_049343_5_2_4"/>
    <dbReference type="OMA" id="EFHALTP"/>
    <dbReference type="OrthoDB" id="1438588at2"/>
    <dbReference type="UniPathway" id="UPA00564">
        <property type="reaction ID" value="UER00628"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047448">
        <property type="term" value="F:5-dehydro-4-deoxyglucarate dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042838">
        <property type="term" value="P:D-glucarate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00951">
        <property type="entry name" value="KDGDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00694">
        <property type="entry name" value="KDGDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002220">
        <property type="entry name" value="DapA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017655">
        <property type="entry name" value="Dehydro-deoxyglucarate_dehyd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12128">
        <property type="entry name" value="PTHR12128"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00701">
        <property type="entry name" value="DHDPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001365">
        <property type="entry name" value="DHDPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01130">
        <property type="entry name" value="DHDPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03249">
        <property type="entry name" value="KdgD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5 6" id="KW-0456">Lyase</keyword>
    <keyword evidence="6" id="KW-0614">Plasmid</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001446"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRNR" id="PIRNR001365"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABF11598.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="304" mass="33122" checksum="885918AAE78C98A5" modified="2006-05-30" version="1">MTTPQELKQIISEGLLSFPITDFDAQGNFRPDTYIERLEWLAPYGASALFAAGGTGEFFSLTQQDYSNVIRTAVETCRGKVPILAGAGGATRVAIEYAKEAERLGAHGVLLMPHYLTEASEEGIANHIEEVCKALKIGVIVYNRANSRIGADLLERVVDRCPNLIGFKDGVGEIEAMVRVRRKLGDRLAYLGGLPTAEVYAAAYKALGVPVYSSAVFNFIPKTAMEFYRAIAADDHATVGRLIDDFFLPYLDIRNRKQGYAVSIVKAGAKLVGHDAGPVRAPLTDLTEDEMARLDALIRKLGPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LEV7</accession>
    <name>Q1LEV7_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Long-chain-fatty-acid-CoA ligase (AMP-dependent synthetase and ligase)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">fadD</name>
        <name evidence="3" type="ordered locus">Rmet_4454</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11319.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518923.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4454"/>
    <dbReference type="EnsemblBacteria" id="ABF11319">
        <property type="protein sequence ID" value="ABF11319"/>
        <property type="gene ID" value="Rmet_4454"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4454"/>
    <dbReference type="eggNOG" id="COG0318">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000022_59_7_4"/>
    <dbReference type="OMA" id="VPTMWIA"/>
    <dbReference type="OrthoDB" id="961884at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016874">
        <property type="term" value="F:ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025110">
        <property type="entry name" value="AMP-bd_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045851">
        <property type="entry name" value="AMP-bd_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020845">
        <property type="entry name" value="AMP-binding_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_Synth/Lig"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13193">
        <property type="entry name" value="AMP-binding_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00455">
        <property type="entry name" value="AMP_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0436">Ligase</keyword>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AMP-binding" evidence="1">
        <location>
            <begin position="32"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="domain" description="AMP-binding_C" evidence="2">
        <location>
            <begin position="472"/>
            <end position="547"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF11319.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="555" mass="60417" checksum="A8A29BB0AA2AC1D6" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-10-07" version="72" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LEX3</accession>
    <name>Q1LEX3_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Extra-cytoplasmic solute receptor (Bug)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">bug</name>
        <name evidence="4" type="ordered locus">Rmet_4438</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="5">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="5">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Periplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the UPF0065 (bug) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11303.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4438"/>
    <dbReference type="EnsemblBacteria" id="ABF11303">
        <property type="protein sequence ID" value="ABF11303"/>
        <property type="gene ID" value="Rmet_4438"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4438"/>
    <dbReference type="eggNOG" id="COG3181">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045683_0_0_4"/>
    <dbReference type="OMA" id="EQGYKDY"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042597">
        <property type="term" value="C:periplasmic space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005064">
        <property type="entry name" value="BUG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042100">
        <property type="entry name" value="Bug_dom1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006311">
        <property type="entry name" value="TAT_signal"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42928">
        <property type="entry name" value="PTHR42928"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03401">
        <property type="entry name" value="TctC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017082">
        <property type="entry name" value="YflP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51318">
        <property type="entry name" value="TAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-0675">Receptor</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193476" evidence="3">
        <location>
            <begin position="31"/>
            <end position="329"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF11303.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="329" mass="35068" checksum="9A33B68807C15590" modified="2006-05-30" version="1">MRRPNLKRRHFLLAPVLGAIALGTATLAHAAYPDRPITLVIPFSAGGPTDVVGRIVASKASEILGQQIVVDNRVGAAGTIGMNAVARAKADGYTLGLATVSTHGTAPHLLAKPPYDPVKDFTPVVNLVSSPNILSVHPGFPARTLKEFVDYTKKHPGKESYANAGAGGINDLGMAWFLQLNDANMISVPYKGSAPALNDAIGGNVKVIFDNFPSSLPFIKAGKLRALAITGTQRNDKLPDVPTFAEQGYKDYDVSAWYGVVAPAKLPPEVRTRLVEAFQAALADANTRQRLVDAGAFPVSVGPDAFARQIRQEYERWGTVIRKAGITPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="101" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LFA7</accession>
    <name>Q1LFA7_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Anion transporter</fullName>
        </submittedName>
        <submittedName>
            <fullName evidence="7">Sodium/sulphate symporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">yflS</name>
        <name evidence="7" type="ordered locus">Rmet_1092</name>
        <name evidence="8" type="ordered locus">Rmet_4303</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="8">
        <name>megaplasmid</name>
    </geneLocation>
    <geneLocation type="plasmid" evidence="9">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="8" key="1">
        <citation type="submission" date="2006-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of the chromosome of Ralstonia metallidurans CH34.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Copeland A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Barry K."/>
                <person name="Detter J.C."/>
                <person name="Glavina del Rio T."/>
                <person name="Hammon N."/>
                <person name="Israni S."/>
                <person name="Dalin E."/>
                <person name="Tice H."/>
                <person name="Martinez M."/>
                <person name="Goltsman E."/>
                <person name="Pitluck S."/>
                <person name="Schmutz J."/>
                <person name="Larimer F."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Kim E."/>
                <person name="Mergeay M."/>
                <person name="Benotmane M.A."/>
                <person name="Vallaeys T."/>
                <person name="Michaux A."/>
                <person name="Monchy S."/>
                <person name="Dunn J."/>
                <person name="McCorkle S."/>
                <person name="Taghavi S."/>
                <person name="van der Lelie D."/>
                <person name="Richardson P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">CH34</strain>
            <plasmid evidence="8">megaplasmid</plasmid>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="9">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2010-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Benotmane R."/>
                <person name="Lapidus A."/>
                <person name="McCorkle S."/>
                <person name="Monchy S."/>
                <person name="Taghavi S."/>
                <person name="van der Lelie N."/>
                <person name="Dunn J."/>
                <person name="Leys N."/>
                <person name="Mergeay M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">CH34</strain>
            <plasmid evidence="8">megaplasmid</plasmid>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SLC13A/DASS transporter (TC 2.A.47) family. DIT1 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07978.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11169.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515879.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1092"/>
    <dbReference type="EnsemblBacteria" id="ABF07978">
        <property type="protein sequence ID" value="ABF07978"/>
        <property type="gene ID" value="Rmet_1092"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF11169">
        <property type="protein sequence ID" value="ABF11169"/>
        <property type="gene ID" value="Rmet_4303"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1092"/>
    <dbReference type="KEGG" id="rme:Rmet_4303"/>
    <dbReference type="eggNOG" id="COG0471">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005170_7_3_4"/>
    <dbReference type="OMA" id="AMAHISA"/>
    <dbReference type="OrthoDB" id="626718at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030676">
        <property type="entry name" value="CitT-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001898">
        <property type="entry name" value="SLC13A/DASS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42826">
        <property type="entry name" value="PTHR42826"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00939">
        <property type="entry name" value="Na_sulph_symp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002457">
        <property type="entry name" value="DASS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00785">
        <property type="entry name" value="dass"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0614">Plasmid</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="20"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="50"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="95"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="126"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="235"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="291"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="317"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="350"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="380"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="409"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="475"/>
            <end position="495"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABF07978.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ABF11169.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="501" mass="53736" checksum="5CF297A4CE865940" modified="2006-05-30" version="1">MFAKIVATYRYFMSVVPFRLTPAIITTVVLVTLLLTPTPAGLKDNAWDLVAIFLTTIVAIILKVMPIGVMAMMAIVIVSLAQVTSNSSKGAIADALSSFDSPLIWLIVVAILISRGLKKTGLGSRIGLMFIALLGKRTVGIGYGLAICELVLAPFTPSNTARGGGIVHPIMRSIAGAFDSDPANGTQGKVGTYLALVNYHANPITSAMFVTATAPNPLVVDYVAKASGQSLHLSWTTWALCMLLPGLVCLLLMPLVIRVLCPPELKATPNAIEYAKGELARMGPLGGKERVMIGVFAMMLVLWANVPAMIWGPTFTLDPTVVAFLGLFALIITGTIDWDDVLSEKSAWDTLIWFGALVMLAEQLNKLGVIAWFSADMRDAIAASGMGWLSIAAILVLAFVFSHYLFASTTAHISAMMLAFLTVGAQLIPQPYIAPFMLMMTAGSAIMMTLTHYATGTSPIIFGSGYVTMGQWWRVGAVMCVFELVIFAVVGGIWWKILGFW</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-10-07" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LFC7</accession>
    <name>Q1LFC7_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">BON domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_4283</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11149.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF11149">
        <property type="protein sequence ID" value="ABF11149"/>
        <property type="gene ID" value="Rmet_4283"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4283"/>
    <dbReference type="HOGENOM" id="CLU_1667955_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007055">
        <property type="entry name" value="BON_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04972">
        <property type="entry name" value="BON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BON" evidence="1">
        <location>
            <begin position="99"/>
            <end position="147"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF04972"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF11149.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="158" mass="17449" checksum="F37BC76426BFC7A2" modified="2006-05-30" version="1">MRRGTWRRRGRLTTDGPVERKHLVSPLSRTVRWQATRGRRKLQHVLHGPRQQSAAPEFEGMPAPVPAVLGLNVTDSIGDTVLPQSDSYLVVVLDERFAQTVDAGTVRAEAHDGIVTLRGTVRDAATCTILETEARNCPGIKAVHSELLARNRSRTNRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LFY5</accession>
    <name>Q1LFY5_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Extra-cytoplasmic solute receptor protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">bug</name>
        <name evidence="4" type="ordered locus">Rmet_4073</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="5">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="5">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Periplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the UPF0065 (bug) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10941.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518566.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4073"/>
    <dbReference type="EnsemblBacteria" id="ABF10941">
        <property type="protein sequence ID" value="ABF10941"/>
        <property type="gene ID" value="Rmet_4073"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4073"/>
    <dbReference type="eggNOG" id="COG3181">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045683_0_1_4"/>
    <dbReference type="OMA" id="FEVGAWQ"/>
    <dbReference type="OrthoDB" id="447734at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042597">
        <property type="term" value="C:periplasmic space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005064">
        <property type="entry name" value="BUG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042100">
        <property type="entry name" value="Bug_dom1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42928">
        <property type="entry name" value="PTHR42928"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03401">
        <property type="entry name" value="TctC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017082">
        <property type="entry name" value="YflP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-0675">Receptor</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193425" evidence="3">
        <location>
            <begin position="34"/>
            <end position="332"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF10941.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="332" mass="35105" checksum="B8CBE1626527E074" modified="2006-05-30" version="1">MPTQSKKAGHSIRSILLAGCAFALTAAVAPAMAGEFPDHPIRLVVPFPPGGPTDLVSRVIARKMSEEIGQQVIVDNRPGANGNIAGEMVAKSPADGYTVLYNTSSIALSPALYKKLPYDVKRDFAPVAMTAMVPLVLEVNAQVPVRTVPEFVAWLKANPGKMTYGSAGNGNVTHLAAFLFLQANGLDATHAPYKGSAPALTDLASGQVQFMTDTINSSLPFIRDKRMRALAVTSSARSSQLPDVPTLAESGMHGFEVGAWQGMMVPAKTSPEIIKKLNSAALKALAAPDVRASLAQQGAEVRASSPEAYAKYVASEIDRWHKVVKDTGVTLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LG32</accession>
    <name>Q1LG32_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Periplasmic ligand-binding sensor protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_4026</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10894.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518533.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4026"/>
    <dbReference type="EnsemblBacteria" id="ABF10894">
        <property type="protein sequence ID" value="ABF10894"/>
        <property type="gene ID" value="Rmet_4026"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4026"/>
    <dbReference type="eggNOG" id="COG3416">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_082335_3_0_4"/>
    <dbReference type="OMA" id="APYGRTN"/>
    <dbReference type="OrthoDB" id="1972954at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018648">
        <property type="entry name" value="DUF2076"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09849">
        <property type="entry name" value="DUF2076"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="76"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="109"/>
            <end position="128"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF10894.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="239" mass="24768" checksum="49F7703D5BA6F2E7" modified="2006-05-30" version="1">MTPQDMQALETFLSQLVQARADGKDPQAAAMIADAAGRQPDAAYLLVQRAMLLDRALVNAQAQIASLQQQLQQMQQGQGSRSFIDDANAWGNHPGRAMPGAAPSARPPYQAAPQYPSTPQPAPQPPQAARPGFLSGGLGGTLGSIATTAAGVAGGAMLFQGIENLFHRNSGGFFGQPAMGSAPTETVINNYYEDGSGSNGGSGGSNALLADNGNLDSGNLDNGLDDFVDDDYRNDDSIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LGL5</accession>
    <name>Q1LGL5_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative peroxidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_3843</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10711.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3843"/>
    <dbReference type="EnsemblBacteria" id="ABF10711">
        <property type="protein sequence ID" value="ABF10711"/>
        <property type="gene ID" value="Rmet_3843"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3843"/>
    <dbReference type="eggNOG" id="COG2128">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_082760_6_2_4"/>
    <dbReference type="OMA" id="SETHAPD"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004601">
        <property type="term" value="F:peroxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051920">
        <property type="term" value="F:peroxiredoxin activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1290.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029032">
        <property type="entry name" value="AhpD-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004675">
        <property type="entry name" value="AhpD_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003779">
        <property type="entry name" value="CMD-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02627">
        <property type="entry name" value="CMD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69118">
        <property type="entry name" value="SSF69118"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00778">
        <property type="entry name" value="ahpD_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="2" id="KW-0575">Peroxidase</keyword>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CMD" evidence="1">
        <location>
            <begin position="36"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02627"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF10711.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="167" mass="18181" checksum="AD28879BA279E8E6" modified="2006-05-30" version="1">MSSSPTQGDETMTARLDPFAAAPVQMKSWMGTSIAIAGSLEPSLVSLVEVRASQINGCANCINMHTTQARERGETEQRLYLLSAWREAPCYTDRERAALGWTEAMTRLSEGHALDRASATLREHFTEEEQVKLTLMINVINGWNRLAVGFGLWVDPAAAKAAAKAAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="118" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LGU1</accession>
    <name>Q1LGU1_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Fused DNA-binding transcriptional dual regulator O6-methylguanine-DNA methyltransferase</fullName>
            <ecNumber evidence="10">2.1.1.63</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">ada</name>
        <name evidence="10" type="ordered locus">Rmet_3765</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="11">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="11">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a 4-O-methyl-thymidine in DNA + L-cysteinyl-[protein] = a thymidine in DNA + S-methyl-L-cysteinyl-[protein]</text>
            <dbReference type="Rhea" id="RHEA:53428"/>
            <dbReference type="Rhea" id="RHEA-COMP:10131"/>
            <dbReference type="Rhea" id="RHEA-COMP:10132"/>
            <dbReference type="Rhea" id="RHEA-COMP:13555"/>
            <dbReference type="Rhea" id="RHEA-COMP:13556"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:82612"/>
            <dbReference type="ChEBI" id="CHEBI:137386"/>
            <dbReference type="ChEBI" id="CHEBI:137387"/>
            <dbReference type="EC" id="2.1.1.63"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>a 6-O-methyl-2'-deoxyguanosine in DNA + L-cysteinyl-[protein] = a 2'-deoxyguanosine in DNA + S-methyl-L-cysteinyl-[protein]</text>
            <dbReference type="Rhea" id="RHEA:24000"/>
            <dbReference type="Rhea" id="RHEA-COMP:10131"/>
            <dbReference type="Rhea" id="RHEA-COMP:10132"/>
            <dbReference type="Rhea" id="RHEA-COMP:11367"/>
            <dbReference type="Rhea" id="RHEA-COMP:11368"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:82612"/>
            <dbReference type="ChEBI" id="CHEBI:85445"/>
            <dbReference type="ChEBI" id="CHEBI:85448"/>
            <dbReference type="EC" id="2.1.1.63"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="8">Binds 1 zinc ion per subunit.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.63" evidence="10"/>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10635.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518282.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3765"/>
    <dbReference type="EnsemblBacteria" id="ABF10635">
        <property type="protein sequence ID" value="ABF10635"/>
        <property type="gene ID" value="Rmet_3765"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3765"/>
    <dbReference type="eggNOG" id="COG0350">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG2169">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_52_0_4"/>
    <dbReference type="OMA" id="RFAIGQC"/>
    <dbReference type="OrthoDB" id="1327994at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003908">
        <property type="term" value="F:methylated-DNA-[protein]-cysteine S-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006304">
        <property type="term" value="P:DNA modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06445">
        <property type="entry name" value="ATase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035451">
        <property type="entry name" value="Ada-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004026">
        <property type="entry name" value="Ada_DNA_repair_Zn-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016221">
        <property type="entry name" value="Bifunct_regulatory_prot_Ada"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018060">
        <property type="entry name" value="HTH_AraC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001497">
        <property type="entry name" value="MethylDNA_cys_MeTrfase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014048">
        <property type="entry name" value="MethylDNA_cys_MeTrfase_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036217">
        <property type="entry name" value="MethylDNA_cys_MeTrfase_DNAb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008332">
        <property type="entry name" value="MethylG_MeTrfase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036631">
        <property type="entry name" value="MGMT_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02805">
        <property type="entry name" value="Ada_Zn_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01035">
        <property type="entry name" value="DNA_binding_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12833">
        <property type="entry name" value="HTH_18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02870">
        <property type="entry name" value="Methyltransf_1N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000409">
        <property type="entry name" value="Ada"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00342">
        <property type="entry name" value="HTH_ARAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46767">
        <property type="entry name" value="SSF46767"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53155">
        <property type="entry name" value="SSF53155"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57884">
        <property type="entry name" value="SSF57884"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00589">
        <property type="entry name" value="ogt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01124">
        <property type="entry name" value="HTH_ARAC_FAMILY_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00374">
        <property type="entry name" value="MGMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0227">DNA damage</keyword>
    <keyword evidence="6" id="KW-0234">DNA repair</keyword>
    <keyword evidence="10" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3 10" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="10" id="KW-0614">Plasmid</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0808">Transferase</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="HTH araC/xylS-type" evidence="9">
        <location>
            <begin position="89"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile; methyl group acceptor from methylphosphotriester" evidence="7">
        <location>
            <position position="39"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile; methyl group acceptor from either O6-methylguanine or O4-methylthymine" evidence="7">
        <location>
            <position position="324"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="39"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001596"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR000409-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR000409-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS01124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ABF10635.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="354" mass="38873" checksum="471F40A78B7B1868" modified="2006-05-30" version="1">MKTTIAYETEDDRWTAVQARDAGADGHFVYAVRTTGVYCQPSSSARLPKRENVEFFDSAEIAEAAGYRCSRRAHGDQTSAAAERAALVARACRMIEASETPPQLDDLATAIGMSPFHFHRLFKAETGLTPKAYSSAYRARKLREELSSPEASITNAIYDSGFNSNSRFYEASDQLLGMRARDYRSGGVGAVIRFAVGQCSLGAILVAQSQRGICAILLGDDPDQLVRDLQDQFRKAEIVGCDGEFEQLIAQVVGFIEAPAMGLHLPLDVQGTAFQERVWRALREIPPGTTVSYAEIAERIGSPKAVRAVAQACATNHIAVAIPCHRVVRRDGDLAGYRWGVDRKRELLRRETQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="115" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LH42</accession>
    <name>Q1LH42_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Putative transcriptional regulator, sigma54-interaction region (Fis family)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Rmet_3662</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="7">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="7">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10534.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518189.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3662"/>
    <dbReference type="EnsemblBacteria" id="ABF10534">
        <property type="protein sequence ID" value="ABF10534"/>
        <property type="gene ID" value="Rmet_3662"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3662"/>
    <dbReference type="eggNOG" id="COG2204">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_7_0_4"/>
    <dbReference type="OMA" id="YWLADAV"/>
    <dbReference type="OrthoDB" id="123059at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002197">
        <property type="entry name" value="HTH_Fis"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002078">
        <property type="entry name" value="Sigma_54_int"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025662">
        <property type="entry name" value="Sigma_54_int_dom_ATP-bd_1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02954">
        <property type="entry name" value="HTH_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00158">
        <property type="entry name" value="Sigma54_activat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01590">
        <property type="entry name" value="HTHFIS"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00675">
        <property type="entry name" value="SIGMA54_INTERACT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50045">
        <property type="entry name" value="SIGMA54_INTERACT_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0614">Plasmid</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Sigma-54 factor interaction" evidence="5">
        <location>
            <begin position="137"/>
            <end position="369"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50045"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABF10534.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="454" mass="50220" checksum="EA2BBBC83EE350B1" modified="2006-05-30" version="1">MKADRWAYESLEVYVWEGKYEIADRVARFLTPLGAEVVRAGALDALPAEPRVKPSIAVISASAIGSAKFTLDWEAAHGMPVIWVGAPDRESDPGRFPPEYAYILPHDFTGADLRTQIAKLMPQLMAAAEARPDVADLVAGSAAMQSLLEQVDTFANFDSNVMLYGETGAGKERIARLFHDRNATYGKGPFVAVNCGAIPDGLFESQFFGHAKGAFTGAMFAHRGFFEQATGGTLFLDEIGDLPLFQQVKLLRVLEENAVTRLGSAMAVKLDFRLVAATNKDLREAVTQGRFRADLYFRLAVIELRLPSLEDRGPADKIGLLQSFMRHMIGAAAYDALPPMPDWLKGAVGSAFFVGNVRELRNLAERIGITVQQRGEWDEARIRPLFRSLRPGAFDGTERADPRGDAEERRRIIAALDANSWRRQDTATCLGISRKVLWEKMRKFQIEDSEAEPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="96" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LH81</accession>
    <name>Q1LH81_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transcriptional regulator, MarR-family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_3623</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10495.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008646910.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3623"/>
    <dbReference type="EnsemblBacteria" id="ABF10495">
        <property type="protein sequence ID" value="ABF10495"/>
        <property type="gene ID" value="Rmet_3623"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3623"/>
    <dbReference type="eggNOG" id="COG1846">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_083287_27_3_4"/>
    <dbReference type="OMA" id="RIDRFCA"/>
    <dbReference type="OrthoDB" id="1744037at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000835">
        <property type="entry name" value="HTH_MarR-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023187">
        <property type="entry name" value="Tscrpt_reg_MarR-type_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01047">
        <property type="entry name" value="MarR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00598">
        <property type="entry name" value="HTHMARR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00347">
        <property type="entry name" value="HTH_MARR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01117">
        <property type="entry name" value="HTH_MARR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50995">
        <property type="entry name" value="HTH_MARR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH marR-type" evidence="1">
        <location>
            <begin position="21"/>
            <end position="155"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF10495.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="165" mass="18332" checksum="4EED98B5A6610047" modified="2006-05-30" version="1">MSFERRIDRFCAEYPEAPRDLILASRLLLRSARLLRQHIERALAVHDLDLNQYLVISMLGIDAMEPTMPSELGLTIDATRTQMTRLIDGLETRGLVQRKASQHDRRSLELILTPAAHDLLKRAAPTVHAAYAEAWAPIGKTGLAAVTRDLGRLHQHLAALGANAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LH92</accession>
    <name>Q1LH92_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Trans-aconitate methyltransferase</fullName>
            <ecNumber evidence="1">2.1.1.144</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">tam</name>
        <name evidence="1" type="ordered locus">Rmet_3612</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.1.1.144" evidence="1"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10484.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518026.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3612"/>
    <dbReference type="EnsemblBacteria" id="ABF10484">
        <property type="protein sequence ID" value="ABF10484"/>
        <property type="gene ID" value="Rmet_3612"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3612"/>
    <dbReference type="eggNOG" id="COG4106">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037990_5_2_4"/>
    <dbReference type="OMA" id="RFDARYY"/>
    <dbReference type="OrthoDB" id="1739932at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030798">
        <property type="term" value="F:trans-aconitate 2-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.290">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023149">
        <property type="entry name" value="Trans_acon_MeTrfase_C"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF10484.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="256" mass="28275" checksum="27A1529DCF50785F" modified="2006-05-30" version="1">MTWSASQYVQFEDERTRPVRDLVGAIPTASARRAADVGCGPGNSTEVLAARYAGAEVTGFDSDADMIAAARKRLPDLRFDIADVTAWNDPGPYDVILANAVFQWVPDHATLFPSLIDKLAPGGSLAVQMPDNLNEPPHQLMRETALNGPWANKLAKAAEARTPLANERWYYELLKPHCTRVDIWRTIYQHPLKGGPAAIVEWFKGSGLRPFLAPLTEDEKAEYLKRYEAAVAKVFPAMSDGTVLLPFPRVFIVATR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="57" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LHA7</accession>
    <name>Q1LHA7_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_3597</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10469.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518012.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3597"/>
    <dbReference type="EnsemblBacteria" id="ABF10469">
        <property type="protein sequence ID" value="ABF10469"/>
        <property type="gene ID" value="Rmet_3597"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3597"/>
    <dbReference type="eggNOG" id="ENOG5032XB1">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_114930_1_0_4"/>
    <dbReference type="OMA" id="MAWYERP"/>
    <dbReference type="OrthoDB" id="1673363at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006311">
        <property type="entry name" value="TAT_signal"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51318">
        <property type="entry name" value="TAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF10469.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="193" mass="20509" checksum="DD4458431F21AC97" modified="2006-05-30" version="1">MPVPAPPTVEKSSRRQFLKVGVGFSAAIACSALLPSLTGCSPASTPPQAGMAFLRPADVALFRALLPAVVSELESVDSVQREKRIAAAVQNIDGSIAAMGLNGRAELRKLLDLLASTPLRWALAGLRAPWDQATPEQVRGFLTRWRTSRFETFNAGGVVLVKLTSVGYFVLPDAWAAAGYPGPNPAVYRAIHS</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-10-07" version="67" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LHG4</accession>
    <name>Q1LHG4_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF2272 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_3540</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10412.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3540"/>
    <dbReference type="EnsemblBacteria" id="ABF10412">
        <property type="protein sequence ID" value="ABF10412"/>
        <property type="gene ID" value="Rmet_3540"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3540"/>
    <dbReference type="eggNOG" id="COG4322">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_982362_0_0_4"/>
    <dbReference type="OMA" id="PMHCDIV"/>
    <dbReference type="OrthoDB" id="682655at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019262">
        <property type="entry name" value="DUF2272"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10030">
        <property type="entry name" value="DUF2272"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF2272" evidence="2">
        <location>
            <begin position="182"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="55"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="106"/>
            <end position="157"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF10030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF10412.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="326" mass="35559" checksum="3F9BF43FC8A7A180" modified="2006-05-30" version="1">MLPRLLPRRFPQRYSQRFPHKAPQSSPQALAQRLTAAIALAVLCGCTTTPPRTARPQAVPFPDYQQVTTPPGATPRERIVDIAMQEWRKWGQQVVHIGRDDSACVTQSPLPAPMTAGPEKASDGGDVSSQASADTENDENGREADCVRFPDGTGMEATPRGCRMAQGYWGIVGEAPDCRQITQGAWAWSAVFISWVMRKAGLDNDQFLTGQSHSMYVVDARDGILPRPAFHIEPMPAVPRPGDVVCAARGRDKYISNVAEIGFGTTPMHCDIVVEVDPAARVVKAIGGNVQQSVSMDVIDMNDAGQLDGFTNSHMPWLLLMRNNLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="96" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LHP8</accession>
    <name>Q1LHP8_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transcriptional regulator, MerR-family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">pbrR3</name>
        <name evidence="2" type="ordered locus">Rmet_3456</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10328.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3456"/>
    <dbReference type="EnsemblBacteria" id="ABF10328">
        <property type="protein sequence ID" value="ABF10328"/>
        <property type="gene ID" value="Rmet_3456"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3456"/>
    <dbReference type="eggNOG" id="COG0789">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_060077_2_0_4"/>
    <dbReference type="OMA" id="FICRARE"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045893">
        <property type="term" value="P:positive regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04784">
        <property type="entry name" value="HTH_CadR-PbrR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011791">
        <property type="entry name" value="CadR-PbrR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009061">
        <property type="entry name" value="DNA-bd_dom_put_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000551">
        <property type="entry name" value="MerR-type_HTH_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13411">
        <property type="entry name" value="MerR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00040">
        <property type="entry name" value="HTHMERR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00422">
        <property type="entry name" value="HTH_MERR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46955">
        <property type="entry name" value="SSF46955"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50937">
        <property type="entry name" value="HTH_MERR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH merR-type" evidence="1">
        <location>
            <begin position="5"/>
            <end position="74"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF10328.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="149" mass="17031" checksum="1286CEBD2B9E9472" modified="2006-05-30" version="1">MEALTMRIGELSRHSGCDIETIRYYEREGLLDAPQREDNGYRRYGDGHLVQLNFVRHCRSLGMSLADVRKLRDFQRNPSLACDDINTLLDRQIEQIHAQRVSLEALEGQLRTLRHTCENPNPHPASECGILQNLQQAAEGAACECHPRH</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LHR8</accession>
    <name>Q1LHR8_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Arsenate reductase</fullName>
            <ecNumber evidence="3">1.20.4.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">yfgD</name>
        <name evidence="4" type="ordered locus">Rmet_3436</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>[glutaredoxin]-dithiol + arsenate + glutathione + H(+) = arsenite + glutathionyl-S-S-[glutaredoxin] + H2O</text>
            <dbReference type="Rhea" id="RHEA:22016"/>
            <dbReference type="Rhea" id="RHEA-COMP:10729"/>
            <dbReference type="Rhea" id="RHEA-COMP:17668"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29242"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:48597"/>
            <dbReference type="ChEBI" id="CHEBI:57925"/>
            <dbReference type="ChEBI" id="CHEBI:146199"/>
            <dbReference type="EC" id="1.20.4.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 2 3">Belongs to the ArsC family.</text>
    </comment>
    <dbReference type="EC" id="1.20.4.1" evidence="3"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10308.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517868.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3436"/>
    <dbReference type="EnsemblBacteria" id="ABF10308">
        <property type="protein sequence ID" value="ABF10308"/>
        <property type="gene ID" value="Rmet_3436"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3436"/>
    <dbReference type="eggNOG" id="COG1393">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_116644_0_1_4"/>
    <dbReference type="OMA" id="MVTHPKL"/>
    <dbReference type="OrthoDB" id="1650947at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008794">
        <property type="term" value="F:arsenate reductase (glutaredoxin) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd03034">
        <property type="entry name" value="ArsC_ArsC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006659">
        <property type="entry name" value="Arsenate_reductase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006660">
        <property type="entry name" value="Arsenate_reductase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30041">
        <property type="entry name" value="PTHR30041"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03960">
        <property type="entry name" value="ArsC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="SSF52833"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00014">
        <property type="entry name" value="arsC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51353">
        <property type="entry name" value="ARSC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU362029"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF10308.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="119" mass="13032" checksum="00A6E85A483C482C" modified="2006-05-30" version="1">MITIYHNPRCSKSRETLALVESAAQRLGEPVEIVEYLKTPPSLATLRQLHTMLGVPVRAMIRDGESAYKELDLADPGLTDAELLAAVADNPILLQRPVVVRNRRAAIGRPPENVAQLLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="106" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LI25</accession>
    <name>Q1LI25_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative transcriptional regulator, GntR-related protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_3329</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">In the C-terminal section; belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10201.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517778.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF10201">
        <property type="protein sequence ID" value="ABF10201"/>
        <property type="gene ID" value="Rmet_3329"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3329"/>
    <dbReference type="eggNOG" id="COG1167">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017584_0_1_4"/>
    <dbReference type="OMA" id="PGLVMGF"/>
    <dbReference type="OrthoDB" id="1320028at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009058">
        <property type="term" value="P:biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07377">
        <property type="entry name" value="WHTH_GntR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000524">
        <property type="entry name" value="Tscrpt_reg_HTH_GntR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00392">
        <property type="entry name" value="GntR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00345">
        <property type="entry name" value="HTH_GNTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="SSF53383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50949">
        <property type="entry name" value="HTH_GNTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH gntR-type" evidence="2">
        <location>
            <begin position="18"/>
            <end position="86"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF10201.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="515" mass="55883" checksum="65BDB2EFEB033DCB" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="113" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LIA6</accession>
    <name>Q1LIA6_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Histidinol dehydrogenase</fullName>
            <shortName evidence="4">HDH</shortName>
            <ecNumber evidence="4">1.1.1.23</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4 11" type="primary">hisD</name>
        <name evidence="11" type="ordered locus">Rmet_3248</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Catalyzes the sequential NAD-dependent oxidations of L-histidinol to L-histidinaldehyde and then to L-histidine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>H2O + L-histidinol + 2 NAD(+) = 3 H(+) + L-histidine + 2 NADH</text>
            <dbReference type="Rhea" id="RHEA:20641"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57595"/>
            <dbReference type="ChEBI" id="CHEBI:57699"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.1.1.23"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="4 9">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="4 9">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="4">Amino-acid biosynthesis; L-histidine biosynthesis; L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 9/9.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 5 10">Belongs to the histidinol dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.23" evidence="4"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10120.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517723.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3248"/>
    <dbReference type="EnsemblBacteria" id="ABF10120">
        <property type="protein sequence ID" value="ABF10120"/>
        <property type="gene ID" value="Rmet_3248"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3248"/>
    <dbReference type="eggNOG" id="COG0141">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006732_3_3_4"/>
    <dbReference type="OMA" id="MVTGPGN"/>
    <dbReference type="OrthoDB" id="935289at2"/>
    <dbReference type="UniPathway" id="UPA00031">
        <property type="reaction ID" value="UER00014"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004399">
        <property type="term" value="F:histidinol dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000105">
        <property type="term" value="P:histidine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd06572">
        <property type="entry name" value="Histidinol_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01024">
        <property type="entry name" value="HisD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016161">
        <property type="entry name" value="Ald_DH/histidinol_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001692">
        <property type="entry name" value="Histidinol_DH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022695">
        <property type="entry name" value="Histidinol_DH_monofunct"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012131">
        <property type="entry name" value="Hstdl_DH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21256">
        <property type="entry name" value="PTHR21256"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00815">
        <property type="entry name" value="Histidinol_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000099">
        <property type="entry name" value="Histidinol_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00083">
        <property type="entry name" value="HOLDHDRGNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53720">
        <property type="entry name" value="SSF53720"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00069">
        <property type="entry name" value="hisD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00611">
        <property type="entry name" value="HISOL_DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="4" id="KW-0368">Histidine biosynthesis</keyword>
    <keyword evidence="1 4 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 7" id="KW-0520">NAD</keyword>
    <keyword evidence="3 4 5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4 9" id="KW-0862">Zinc</keyword>
    <feature type="active site" description="Proton acceptor" evidence="4 6">
        <location>
            <position position="341"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="4 6">
        <location>
            <position position="342"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4 7">
        <location>
            <position position="144"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4 7">
        <location>
            <position position="205"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4 7">
        <location>
            <position position="228"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4 8">
        <location>
            <position position="251"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4 8 9">
        <location>
            <position position="273"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4 8 9">
        <location>
            <position position="276"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4 8">
        <location>
            <position position="342"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4 8 9">
        <location>
            <position position="375"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4 8">
        <location>
            <position position="429"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4 8 9">
        <location>
            <position position="434"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRNR" id="PIRNR000099"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR000099-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR000099-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR000099-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR000099-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU004175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ABF10120.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="444" mass="48104" checksum="5F9AE1FB337BE8E2" modified="2006-05-30" version="1">MNDTEMENLSIRRLDSREPGFAGALRQVLAFEASEDEAIDRAAADILADVRRRGDEAVLEYTRRFDRMEASSMSALALSQSELEAALEDLEPKRRAALEAAAARVRAYHEKQKIECGSHSWEYTEADGTMLGQKVTPLDRVGLYVPGGKAAYPSSVLMNAIPARVAGVKEVIMVVPTPGGVRNELVLAAAQIAGVDRVYTIGGAQAVGALAYGTATLPQVDKIVGPGNAYVAAAKRRVFGTVGIDMIAGPSEILVICDGTTDPDWVAMDLFSQAEHDELAQSILLCPDAEYLNRVLESIQRQLPTMPRREVIAASLSGRGALVKVRDMEEACEIANAIAPEHLEISAENPRQWSEKIRHAGAIFLGRYTSESLGDYCAGPNHVLPTSRTARFSSPLGVYDFQKRSSLIEVSENGAQMLGQIAAELAYGEGLQAHARSAEYRFKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LID8</accession>
    <name>Q1LID8_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Transcriptional regulator, LysR-family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_3216</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the LysR transcriptional regulatory family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10088.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517698.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3216"/>
    <dbReference type="EnsemblBacteria" id="ABF10088">
        <property type="protein sequence ID" value="ABF10088"/>
        <property type="gene ID" value="Rmet_3216"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3216"/>
    <dbReference type="eggNOG" id="COG0583">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039613_16_2_4"/>
    <dbReference type="OMA" id="REICDIR"/>
    <dbReference type="OrthoDB" id="1661500at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005119">
        <property type="entry name" value="LysR_subst-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000847">
        <property type="entry name" value="Tscrpt_reg_HTH_LysR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00126">
        <property type="entry name" value="HTH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03466">
        <property type="entry name" value="LysR_substrate"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50931">
        <property type="entry name" value="HTH_LYSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH lysR-type" evidence="2">
        <location>
            <begin position="1"/>
            <end position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF10088.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="306" mass="34043" checksum="638CCBBFC3248AC8" modified="2006-05-30" version="1">MDLHLVQAFVDIVEAGNLAEAGRRRGVTRSQISRQLGQLEEQAGAMLLRRTTRRLEMTDAGQSLYEHGLRILQEVAAAQAEIDSLGKTLRGHVRVSVPTGLGDAFIAPLLLQFAEKHPGISLRVFFANRVTDLIAAEIDVALKVTSEPPLDTVARDVCPIHWQLCASPAYLAGIAPIREPADLAACRFLCPPYTSRRFLLTLGRDTCDGPEREDVDITPYLQSEHFPFLMNAVRQGHGISLLPLYMTWEDVRRGTLVPVLPDWKPKGLGNRLYIFTTANPHLSMATRALISFLRETVAELDVFKER</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="59" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LIW9</accession>
    <name>Q1LIW9_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">F plasmid protein 32-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">yupB</name>
        <name evidence="2" type="ordered locus">Rmet_3034</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09907.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_003056211.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3034"/>
    <dbReference type="EnsemblBacteria" id="ABF09907">
        <property type="protein sequence ID" value="ABF09907"/>
        <property type="gene ID" value="Rmet_3034"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3034"/>
    <dbReference type="eggNOG" id="COG1961">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_059317_0_0_4"/>
    <dbReference type="OMA" id="TPRRYED"/>
    <dbReference type="OrthoDB" id="716673at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026325">
        <property type="entry name" value="DUF932"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06067">
        <property type="entry name" value="DUF932"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF09907.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="275" mass="30785" checksum="2D5042B7B134C1B1" modified="2006-05-30" version="1">MQLASRFASHSPALRSDSPLSDDQIRRVAPSIFADAPHESRSERYSYIPTAAVLTELRKEGFQPFMVCQTRVRNEGRREHTKHMLRLRHANQINAREANEIILLNSHDGTSSYQLLGGMFRFVCSNGLVCGDTVGDVRVPHKGDVAGHVIEGAYQVLSGFEHAQESRESMQAITLDAGESEVFARAALALKYDDPTKPAPVTESQILMPRRFDDRRPDLWSVFNRTQENLTKGGLHGRAANGRRQQTRPVQGIDSDIRLNRALWLLADGMRALKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LJ73</accession>
    <name>Q1LJ73_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Effector of murein hydrolase LrgA</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">lrgA</name>
        <name evidence="7" type="ordered locus">Rmet_2930</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09803.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008641946.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2930"/>
    <dbReference type="EnsemblBacteria" id="ABF09803">
        <property type="protein sequence ID" value="ABF09803"/>
        <property type="gene ID" value="Rmet_2930"/>
    </dbReference>
    <dbReference type="GeneID" id="60820634"/>
    <dbReference type="KEGG" id="rme:Rmet_2930"/>
    <dbReference type="eggNOG" id="COG1380">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_113736_4_3_4"/>
    <dbReference type="OMA" id="MLQTFAI"/>
    <dbReference type="OrthoDB" id="1846131at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005538">
        <property type="entry name" value="LrgA/CidA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33931">
        <property type="entry name" value="PTHR33931"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03788">
        <property type="entry name" value="LrgA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="31"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="57"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="83"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABF09803.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="128" mass="13745" checksum="6FA44FE599CEAC3B" modified="2006-05-30" version="1">MLQTFAILLVFQTVGEVISYALHLPVPGPVIGMLLLFGWLVFDNRLLPVVQNSANELLKHLSLLFVPAGVGIMVHANRIGSEWLPIVIALVVSTWVAIAVTALVTRALMRKPPADTPVDTSADTGAQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="113" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LJA3</accession>
    <name>Q1LJA3_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7 19">UDP-N-acetylenolpyruvoylglucosamine reductase</fullName>
            <ecNumber evidence="6 19">1.3.1.98</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="18 19">UDP-N-acetylmuramate dehydrogenase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19 21" type="primary">murB</name>
        <name evidence="21" type="ordered locus">Rmet_2900</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="22">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3 19">Cell wall formation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 19">
            <text>NADP(+) + UDP-N-acetyl-alpha-D-muramate = H(+) + NADPH + UDP-N-acetyl-3-O-(1-carboxyvinyl)-alpha-D-glucosamine</text>
            <dbReference type="Rhea" id="RHEA:12248"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:68483"/>
            <dbReference type="ChEBI" id="CHEBI:70757"/>
            <dbReference type="EC" id="1.3.1.98"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 19">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="5 19">Cell wall biogenesis; peptidoglycan biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4 19">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="19">Belongs to the MurB family.</text>
    </comment>
    <dbReference type="EC" id="1.3.1.98" evidence="6 19"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09773.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517449.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2900"/>
    <dbReference type="EnsemblBacteria" id="ABF09773">
        <property type="protein sequence ID" value="ABF09773"/>
        <property type="gene ID" value="Rmet_2900"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2900"/>
    <dbReference type="eggNOG" id="COG0812">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035304_0_0_4"/>
    <dbReference type="OMA" id="MQNIGAY"/>
    <dbReference type="OrthoDB" id="841869at2"/>
    <dbReference type="UniPathway" id="UPA00219"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008762">
        <property type="term" value="F:UDP-N-acetylmuramate dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007049">
        <property type="term" value="P:cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009252">
        <property type="term" value="P:peptidoglycan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008360">
        <property type="term" value="P:regulation of cell shape"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.43.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.465.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.78.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00037">
        <property type="entry name" value="MurB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016166">
        <property type="entry name" value="FAD-bd_PCMH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036318">
        <property type="entry name" value="FAD-bd_PCMH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016167">
        <property type="entry name" value="FAD-bd_PCMH_sub1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016169">
        <property type="entry name" value="FAD-bd_PCMH_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003170">
        <property type="entry name" value="MurB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011601">
        <property type="entry name" value="MurB_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036635">
        <property type="entry name" value="MurB_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006094">
        <property type="entry name" value="Oxid_FAD_bind_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21071">
        <property type="entry name" value="PTHR21071"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01565">
        <property type="entry name" value="FAD_binding_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02873">
        <property type="entry name" value="MurB_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56176">
        <property type="entry name" value="SSF56176"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56194">
        <property type="entry name" value="SSF56194"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00179">
        <property type="entry name" value="murB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51387">
        <property type="entry name" value="FAD_PCMH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="16 19" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="9 19" id="KW-0132">Cell division</keyword>
    <keyword evidence="13 19" id="KW-0133">Cell shape</keyword>
    <keyword evidence="17 19" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="8 19" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11 19" id="KW-0274">FAD</keyword>
    <keyword evidence="10 19" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="12 19" id="KW-0521">NADP</keyword>
    <keyword evidence="15 19 21" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="14 19" id="KW-0573">Peptidoglycan synthesis</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD-binding PCMH-type" evidence="20">
        <location>
            <begin position="18"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="active site" evidence="19">
        <location>
            <position position="164"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="19">
        <location>
            <position position="236"/>
        </location>
    </feature>
    <feature type="active site" evidence="19">
        <location>
            <position position="332"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00003921"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004752"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012518"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00015188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00031026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS51387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="ABF09773.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="336" mass="36208" checksum="A9AD6EEA143BBB28" modified="2006-05-30" version="1">MADFLEFYPLRTHNTFGFDARARLAVHIRNESDLVSALSDHRIDNLPIVVLGGGSNVVLTGDLDACVLLMEIPGYDVAEDDASWLVTAGAGETWHGLVNRTIDEGMPGLENLALIPGTAGAAPIQNIGAYGVELRERFARLRAYDRQSGDFVTIDLDTCAFGYRDSLFKREGRDRYIITAVTLRLPKAWQPVLNYGELTRELEGVSTPDAAQVRDAIVAIRSRKLPDPAKIGNAGSFFKNPVVDASLRNALLADHPALVSYPQPDGSYKLAAGWLIDQCGFKGVDDGPVGVYGKQALVLVHHGGGTGAALLALANRIADAVQAHFGVRIEPEPVIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-12-02" version="69" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LJA5</accession>
    <name>Q1LJA5_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_2898</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09771.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2898"/>
    <dbReference type="EnsemblBacteria" id="ABF09771">
        <property type="protein sequence ID" value="ABF09771"/>
        <property type="gene ID" value="Rmet_2898"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2898"/>
    <dbReference type="eggNOG" id="COG1430">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_097039_0_0_4"/>
    <dbReference type="OMA" id="TMWMKNT"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.1140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003795">
        <property type="entry name" value="DUF192"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038695">
        <property type="entry name" value="Saro_0823-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37953">
        <property type="entry name" value="PTHR37953"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02643">
        <property type="entry name" value="DUF192"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF09771.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="160" mass="17264" checksum="B7A5A25D27FF2D26" modified="2006-05-30" version="1">MLSAAVDPLTSDNAMHRMIKTLLAAGTTVLATLAHAQSALPVIPLTAGMYAIQAEVAASPEARERGLMFRKNMPANAGMLFVFEDKAGHCFWMKNTDLPLSIAFLADDGSIVNIEEMKPQTEDNHCPRAAVRYALEMNKGWFAQKGLKPGAKIGGLPQPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="98" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LJA7</accession>
    <name>Q1LJA7_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Pseudouridine synthase</fullName>
            <ecNumber evidence="3">5.4.99.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">rluE</name>
        <name evidence="6" type="ordered locus">Rmet_2896</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the pseudouridine synthase RsuA family.</text>
    </comment>
    <dbReference type="EC" id="5.4.99.-" evidence="3"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09769.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517446.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2896"/>
    <dbReference type="EnsemblBacteria" id="ABF09769">
        <property type="protein sequence ID" value="ABF09769"/>
        <property type="gene ID" value="Rmet_2896"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2896"/>
    <dbReference type="eggNOG" id="COG1187">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024979_8_1_4"/>
    <dbReference type="OMA" id="HPRTYWV"/>
    <dbReference type="OrthoDB" id="1037615at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140098">
        <property type="term" value="F:catalytic activity, acting on RNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009982">
        <property type="term" value="F:pseudouridine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034470">
        <property type="term" value="P:ncRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001522">
        <property type="term" value="P:pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1560">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.580">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042092">
        <property type="entry name" value="PsdUridine_s_RsuA/RluB/E/F_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020103">
        <property type="entry name" value="PsdUridine_synth_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006145">
        <property type="entry name" value="PsdUridine_synth_RsuA/RluA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000748">
        <property type="entry name" value="PsdUridine_synth_RsuA/RluB/E/F"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018496">
        <property type="entry name" value="PsdUridine_synth_RsuA/RluB_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020094">
        <property type="entry name" value="TruA/RsuA/RluB/E/F_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00849">
        <property type="entry name" value="PseudoU_synth_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55120">
        <property type="entry name" value="SSF55120"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00093">
        <property type="entry name" value="TIGR00093"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01149">
        <property type="entry name" value="PSI_RSU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0413">Isomerase</keyword>
    <keyword evidence="6" id="KW-0456">Lyase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PseudoU_synth_2" evidence="5">
        <location>
            <begin position="3"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="188"/>
            <end position="214"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008348"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABF09769.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="214" mass="23510" checksum="EB3586B73D248E16" modified="2006-05-30" version="1">MTLIALNKPYQTMSQFSAHPSRATLADCVDVPGVYPAGRLDADSEGLLLLTDDGALQARIADPRHKLVKTYLAQVEGVPDEAALATLRTGVDLGDFKTLPAKARVVDEPAWLWERHPPIRYRAAIPTTWIELSIREGKNRQVRRMTAAVGLPTLRLVRISIGNLKLPELGLMPGQWCETDVSRLGLSSGNPHVSTARPERAPTSGPARRSYKFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="95" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LJE5</accession>
    <name>Q1LJE5_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Taurine ABC-type transporter, permease component: TauT family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">tauC</name>
        <name evidence="6" type="ordered locus">Rmet_2858</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cell membrane</location>
            <topology evidence="4">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the binding-protein-dependent transport system permease family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09731.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517415.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2858"/>
    <dbReference type="EnsemblBacteria" id="ABF09731">
        <property type="protein sequence ID" value="ABF09731"/>
        <property type="gene ID" value="Rmet_2858"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2858"/>
    <dbReference type="eggNOG" id="COG0600">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046113_1_4_4"/>
    <dbReference type="OMA" id="GIGHMIV"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="SSF161098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2 4" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="46"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="101"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="137"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="163"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="227"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="259"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="5">
        <location>
            <begin position="97"/>
            <end position="277"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABF09731.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="291" mass="31331" checksum="EC6A621B7E9C4969" modified="2006-05-30" version="1">MSQASTPTTAAAARVPAPIEAQGAPARRARHPWMSPLVPVSPRARWLLGMSFFVAFFAVWAVATLGGFVSRTFLADPLTMAHEGWRLFTQYGFIGDIGMTVWRVVGGFVLAALLAVPLGIFMGAYKAAEAFFEPFVSFCRYLPASAFIPLLILWAGIGETQKLLVIFIGSFFQIVLMVAVTVGGARKDLVEAAYTLGATPRGIVRRVLIPGAAPDIAETLRLVLGWAWTYVIVAELIGSSSGIGHMITDSQALLNTGQIIFGIIVIGCIGLVSDLVFKQANQRLFPWSSIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LJI1</accession>
    <name>Q1LJI1_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative arylformamidase</fullName>
            <ecNumber evidence="2">3.5.1.9</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_2822</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EC" id="3.5.1.9" evidence="2"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09695.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517388.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2822"/>
    <dbReference type="EnsemblBacteria" id="ABF09695">
        <property type="protein sequence ID" value="ABF09695"/>
        <property type="gene ID" value="Rmet_2822"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2822"/>
    <dbReference type="eggNOG" id="COG0657">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012494_4_7_4"/>
    <dbReference type="OMA" id="LEAAEYH"/>
    <dbReference type="OrthoDB" id="1470522at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004061">
        <property type="term" value="F:arylformamidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013094">
        <property type="entry name" value="AB_hydrolase_3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07859">
        <property type="entry name" value="Abhydrolase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="SSF53474"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Abhydrolase_3" evidence="1">
        <location>
            <begin position="99"/>
            <end position="242"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF07859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF09695.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="313" mass="34334" checksum="9FA10C35124535EB" modified="2006-05-30" version="1">MSVQPPLDVAPAATFAALAELPSQDPAFYAQQYNNRANVPENPVHMARWAADSALARAGAIEYFEDLSYGEVDSSLVATETYDYFPAMHVPDGTRPPLLVFLHGGYYRALDKRDHSFVASVPTRRGVSVAVVNYALCPDVTVETIVRQALEAVAWLYRQADALGHDPDRIFLAGHSVGGHLVTMLMTALWPQVGADLPADLVKGGLSISGLYDLEPLRRTEFLQADLKLTDSDVARMSPAFMLPATRAPLITAVGELESSEYHRHNALIHAAWPDNVREQIALPGRHHFNAMDDLMRDDSPMMRALLGMIAGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LJM1</accession>
    <name>Q1LJM1_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Vitamin B12 transporter subunit: periplasmic-binding component of ABC superfamily Cobalamin-binding protein (BtuF)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">btuF</name>
        <name evidence="3" type="ordered locus">Rmet_2782</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09655.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517353.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2782"/>
    <dbReference type="EnsemblBacteria" id="ABF09655">
        <property type="protein sequence ID" value="ABF09655"/>
        <property type="gene ID" value="Rmet_2782"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2782"/>
    <dbReference type="eggNOG" id="COG0614">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038034_2_5_4"/>
    <dbReference type="OMA" id="WQGINLE"/>
    <dbReference type="OrthoDB" id="1473468at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002491">
        <property type="entry name" value="ABC_transptr_periplasmic_BD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01497">
        <property type="entry name" value="Peripla_BP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50983">
        <property type="entry name" value="FE_B12_PBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193165" evidence="1">
        <location>
            <begin position="23"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="domain" description="Fe/B12 periplasmic-binding" evidence="2">
        <location>
            <begin position="42"/>
            <end position="295"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF09655.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="300" mass="32261" checksum="55451C30D9147FEC" modified="2006-05-30" version="1">MKAFYVASAASALLIAAMPAHAAVSVVDDAGETVTLAQPARRIVSLAPHVTELIFAAGGGDRIVGTVSYSDYPPQARDIPRVGDNKALDLERIAALKPDLIVVWRHGNAQKQTDRLRALGMPLFFSEPRKLDGIADNLEKLGTLMGTTPVAHRAATDFRQQVSTLRKTYAGRPPVTVFYQVWQQPLMTLNGEHMVSDLLALCGGRNLFGKEAALVPTVSVEAVVAGNPEVMLTAGMGATRSDKPLADFAMWEKWKQVTAVARNNLFVIDGDLVNRAGPRVAQGAAEICKDLDVARSRRPG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-01-15" modified="2022-05-25" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LJQ2</accession>
    <name>ARLY_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Argininosuccinate lyase</fullName>
            <shortName evidence="1">ASAL</shortName>
            <ecNumber evidence="1">4.3.2.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Arginosuccinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">argH</name>
        <name type="ordered locus">Rmet_2751</name>
    </gene>
    <organism>
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>2-(N(omega)-L-arginino)succinate = fumarate + L-arginine</text>
            <dbReference type="Rhea" id="RHEA:24020"/>
            <dbReference type="ChEBI" id="CHEBI:29806"/>
            <dbReference type="ChEBI" id="CHEBI:32682"/>
            <dbReference type="ChEBI" id="CHEBI:57472"/>
            <dbReference type="EC" id="4.3.2.1"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-arginine biosynthesis; L-arginine from L-ornithine and carbamoyl phosphate: step 3/3.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the lyase 1 family. Argininosuccinate lyase subfamily.</text>
    </comment>
    <dbReference type="EC" id="4.3.2.1" evidence="1"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09624.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517324.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q1LJQ2"/>
    <dbReference type="SMR" id="Q1LJQ2"/>
    <dbReference type="STRING" id="266264.Rmet_2751"/>
    <dbReference type="EnsemblBacteria" id="ABF09624">
        <property type="protein sequence ID" value="ABF09624"/>
        <property type="gene ID" value="Rmet_2751"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2751"/>
    <dbReference type="eggNOG" id="COG0165">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027272_2_3_4"/>
    <dbReference type="OMA" id="KKNPDVF"/>
    <dbReference type="OrthoDB" id="751464at2"/>
    <dbReference type="UniPathway" id="UPA00068">
        <property type="reaction ID" value="UER00114"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004056">
        <property type="term" value="F:argininosuccinate lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042450">
        <property type="term" value="P:arginine biosynthetic process via ornithine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01359">
        <property type="entry name" value="Argininosuccinate_lyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.275.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00006">
        <property type="entry name" value="Arg_succ_lyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029419">
        <property type="entry name" value="Arg_succ_lyase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009049">
        <property type="entry name" value="Argininosuccinate_lyase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024083">
        <property type="entry name" value="Fumarase/histidase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020557">
        <property type="entry name" value="Fumarate_lyase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000362">
        <property type="entry name" value="Fumarate_lyase_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022761">
        <property type="entry name" value="Fumarate_lyase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008948">
        <property type="entry name" value="L-Aspartase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43814">
        <property type="entry name" value="PTHR43814"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14698">
        <property type="entry name" value="ASL_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00206">
        <property type="entry name" value="Lyase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00149">
        <property type="entry name" value="FUMRATELYASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48557">
        <property type="entry name" value="SSF48557"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00838">
        <property type="entry name" value="argH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00163">
        <property type="entry name" value="FUMARATE_LYASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0055">Arginine biosynthesis</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0456">Lyase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_1000000530" description="Argininosuccinate lyase">
        <location>
            <begin position="1"/>
            <end position="469"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00006"/>
        </source>
    </evidence>
    <sequence length="469" mass="51367" checksum="2E1DF9AD69095A07" modified="2006-05-30" version="1">MTSQLAKKGEAWSARFSEPMSDLVKRYTASVFFDKRLALFDIQGSLAHAAMLAKQGIIAEADRAEIERGMTQIRGEIESGAFEWKLDLEDVHLNIEARLTALVGDAGKRLHTGRSRNDQVATDIRLWLRSEIDSIVGLLGDLRGALLDLAEKNADTILPGFTHLQVAQPVTFGHHLMAYVEMFTRDAERMADCRRRVNRLPLGAAALAGTSYPIDREFVATQLGFDGVCRNSLDAVSDRDFAIEFLAAASLIMTHVSRFSEELVIWMSPRVGFIDIADRFCTGSSIMPQKKNPDVPELARGKTGRVYGHLTGLLTLMKGQPLAYNKDNQEDKEPLFDTVDTVVDTLRIFADMVPGISVKPDNMRAAALQGYATATDLADYLVKRGLPFRDAHEAVAHAVRACDDRHCDLADLTVEQLREVSGLGDKASLIGDDVHSVLTLEGSVASRNHIGGTAPDQVRAAIAAARKAL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2007-05-29" modified="2022-08-03" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LJU9</accession>
    <name>TRMB_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">tRNA (guanine-N(7)-)-methyltransferase</fullName>
            <ecNumber evidence="2">2.1.1.33</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">tRNA (guanine(46)-N(7))-methyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="2">tRNA(m7G46)-methyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="2" type="primary">trmB</name>
        <name type="ordered locus">Rmet_2704</name>
    </gene>
    <organism>
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>guanosine(46) in tRNA + S-adenosyl-L-methionine = N(7)-methylguanosine(46) in tRNA + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:42708"/>
            <dbReference type="Rhea" id="RHEA-COMP:10188"/>
            <dbReference type="Rhea" id="RHEA-COMP:10189"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:74269"/>
            <dbReference type="ChEBI" id="CHEBI:74480"/>
            <dbReference type="EC" id="2.1.1.33"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">tRNA modification; N(7)-methylguanine-tRNA biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.33" evidence="2"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09577.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517275.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q1LJU9"/>
    <dbReference type="SMR" id="Q1LJU9"/>
    <dbReference type="STRING" id="266264.Rmet_2704"/>
    <dbReference type="EnsemblBacteria" id="ABF09577">
        <property type="protein sequence ID" value="ABF09577"/>
        <property type="gene ID" value="Rmet_2704"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2704"/>
    <dbReference type="eggNOG" id="COG0220">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_050910_0_1_4"/>
    <dbReference type="OMA" id="PDPWHKS"/>
    <dbReference type="OrthoDB" id="1025521at2"/>
    <dbReference type="UniPathway" id="UPA00989"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008176">
        <property type="term" value="F:tRNA (guanine-N7-)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01057">
        <property type="entry name" value="tRNA_methyltr_TrmB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003358">
        <property type="entry name" value="tRNA_(Gua-N-7)_MeTrfase_Trmb"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23417">
        <property type="entry name" value="PTHR23417"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02390">
        <property type="entry name" value="Methyltransf_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00091">
        <property type="entry name" value="TIGR00091"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51625">
        <property type="entry name" value="SAM_MT_TRMB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0489">Methyltransferase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <keyword id="KW-0819">tRNA processing</keyword>
    <feature type="chain" id="PRO_0000288208" description="tRNA (guanine-N(7)-)-methyltransferase">
        <location>
            <begin position="1"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="region of interest" description="Interaction with RNA" evidence="2">
        <location>
            <begin position="173"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="167"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="92"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="117"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="144"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="167"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="171"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="203"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="241"/>
            <end position="244"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="262" mass="29030" checksum="325EF93273C5BACB" modified="2006-05-30" version="1">MLPQDTNTDPLPGDDAESASGKSADASQGTPNPGDEVAHPRRIRSFVRRAGRTSTGQQRAMDELGPTYIVPYQPEPLDWTATFGREAPSILEIGFGMGETTAHIAGLRSQDNFLGCEVHEPGVGALLKLIGERGLQNVRIMQHDAVEVAAHMLTENSLDGVHIYFPDPWHKKRHNKRRLVQPPLVKLLASRLKPGGYIHCATDWEEYAHQMVEVLSGEPALENTSKADGGFAERPDYRPVTKFEKRGLRLGHGVWDVVFRKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-10-07" version="70" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LJW2</accession>
    <name>Q1LJW2_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">AB hydrolase-1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_2691</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09564.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2691"/>
    <dbReference type="ESTHER" id="ralme-q1ljw2">
        <property type="family name" value="6_AlphaBeta_hydrolase"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF09564">
        <property type="protein sequence ID" value="ABF09564"/>
        <property type="gene ID" value="Rmet_2691"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2691"/>
    <dbReference type="eggNOG" id="COG1073">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_086027_0_0_4"/>
    <dbReference type="OMA" id="DWIATRK"/>
    <dbReference type="OrthoDB" id="1544202at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00561">
        <property type="entry name" value="Abhydrolase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="SSF53474"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193227" description="AB hydrolase-1 domain-containing protein" evidence="1">
        <location>
            <begin position="23"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="domain" description="AB hydrolase-1" evidence="2">
        <location>
            <begin position="111"/>
            <end position="192"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF09564.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="282" mass="30872" checksum="EA1C34EFF23D0D8F" modified="2006-05-30" version="1">MRYVMTIVALCLGVFLSPRTMAQEPVLEESVTTVSLPRGTELSLVVSKKPGSSPSTAALLFPGYPGVLRVEVQNGAPVYQLRGNFLVRARRHLVSDQVMTVMVDCPKDRWSNCDDDYRTSDQYAVDVGAAIDKLKADLGIGKVYLIGTSYGTVSSAFLARKLDGRIDGAVHTSTITDPRAGRNRNAHGLPMWNFDWTATHVDQLFVHHQDDPCPLTQYRSIVARRGNIPLITVLGSKGARGEPCEAFSQHGFVGREQVVMRAIGDWIATRKAAETVGEKGDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="54" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LJY7</accession>
    <name>Q1LJY7_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_2666</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09539.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2666"/>
    <dbReference type="EnsemblBacteria" id="ABF09539">
        <property type="protein sequence ID" value="ABF09539"/>
        <property type="gene ID" value="Rmet_2666"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2666"/>
    <dbReference type="eggNOG" id="ENOG5033WMT">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_120842_0_0_4"/>
    <dbReference type="OMA" id="LRICKQH"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF09539.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="212" mass="23424" checksum="660A1844F5F192B7" modified="2006-05-30" version="1">MGITRVEGEAKNQDYFMAGRLADSSGYTWTLPTRLSEMLCEAEGLFGRRDLSWTILGVEFGPGPQPQIWYPMNHDGRRHVLIQLAPNALLDGAIACYQLAHEVVHLLAPNPGVMAPVIEEGAATVFSEDYTERVFGRRNMTSSQTYRAAAQVVRELLALDPAAIKKMREVEPAFTNFTLETFEKAGLKAPQLLKEALLTPFSEVLAVTQLAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LK12</accession>
    <name>Q1LK12_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Hemerythrin-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Rmet_2637</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the hemerythrin family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09514.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517213.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2637"/>
    <dbReference type="EnsemblBacteria" id="ABF09514">
        <property type="protein sequence ID" value="ABF09514"/>
        <property type="gene ID" value="Rmet_2637"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2637"/>
    <dbReference type="eggNOG" id="COG2703">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_086902_1_0_4"/>
    <dbReference type="OMA" id="QEDRWMQ"/>
    <dbReference type="OrthoDB" id="1991725at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd12107">
        <property type="entry name" value="Hemerythrin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016131">
        <property type="entry name" value="Haemerythrin_Fe_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012312">
        <property type="entry name" value="Hemerythrin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035938">
        <property type="entry name" value="Hemerythrin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012827">
        <property type="entry name" value="Hemerythrin_metal-bd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01814">
        <property type="entry name" value="Hemerythrin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47188">
        <property type="entry name" value="SSF47188"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00550">
        <property type="entry name" value="HEMERYTHRINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0408">Iron</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Hemerythrin" evidence="4">
        <location>
            <begin position="32"/>
            <end position="144"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010587"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABF09514.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="160" mass="17749" checksum="18B257A8E67E45E3" modified="2006-05-30" version="1">MTNTDSYADPYAGLSAEGLPDDLRLGEPVTDATHAEFLQLLAAMEKADDAGFLAAFEEWIEHTRHHFEQEEQWMEAMQFGPRFCHSNEHQGVMSVAKAVRDKIVEERADPEGPGKGALSLGRRLTGELSGWFDNHVKSMDTMMVEHMKANGFSLVEAPAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LK61</accession>
    <name>Q1LK61_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">2-keto-3-deoxygluconate permease</fullName>
            <shortName evidence="9">KDG permease</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9 10" type="primary">kdgT</name>
        <name evidence="10" type="ordered locus">Rmet_2588</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8 9">The 2-keto-3-deoxygluconate permease transports the degraded pectin products into the bacterial cell, where they serve as carbon and energy sources. This is a hydrogen coupled transport system.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cell inner membrane</location>
            <topology evidence="9">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the KdgT transporter family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09465.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008649893.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2588"/>
    <dbReference type="EnsemblBacteria" id="ABF09465">
        <property type="protein sequence ID" value="ABF09465"/>
        <property type="gene ID" value="Rmet_2588"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2588"/>
    <dbReference type="eggNOG" id="ENOG502Z7JT">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_057476_0_0_4"/>
    <dbReference type="OMA" id="VMIPFFA"/>
    <dbReference type="OrthoDB" id="1029737at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015649">
        <property type="term" value="F:2-keto-3-deoxygluconate:proton symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00070">
        <property type="entry name" value="KdgT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004684">
        <property type="entry name" value="2keto-3dGluconate_permease"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03812">
        <property type="entry name" value="KdgT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="4 9" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0762">Sugar transport</keyword>
    <keyword evidence="9" id="KW-0769">Symport</keyword>
    <keyword evidence="5 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 9" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="44"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="78"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="103"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="141"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="168"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="196"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="222"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="253"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="288"/>
            <end position="311"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00024880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ABF09465.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="327" mass="33337" checksum="40CD87BE13ACF9D8" modified="2006-05-30" version="1">MQIHIKRAIERVPGGMMIVPLLIGSLVATFAPDGPKFFGSFTNALFTGALPILAVFYVCMGASINIKATPYIVKKGGVLFATKVGTAMLLGVILGHFLGESPISAGMFAGLSTLAVVAAMNDTNGGLYMALMGQYGRPEDVGAYSIMSLESGPFLTMVTLGVAGLSAFPWPTLVGSILPLMLGMLLGNLDREMRDFLSKAVPVMIPFFALALGAGLDLHKVWQAGMLGLGLGVAVVVVTGFALYIADRLSGGTGVAGVAAASTAGNAAAVPTLVAAANPAYTEAAKSATILVAACVVVTAVLTPLVTAFVARRVANRTSAAVARTTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LKD6</accession>
    <name>Q1LKD6_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Aminotransferase family protein</fullName>
            <ecNumber evidence="2">2.6.1.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_2513</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.6.1.-" evidence="2"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09390.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2513"/>
    <dbReference type="EnsemblBacteria" id="ABF09390">
        <property type="protein sequence ID" value="ABF09390"/>
        <property type="gene ID" value="Rmet_2513"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2513"/>
    <dbReference type="eggNOG" id="COG1167">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017584_0_6_4"/>
    <dbReference type="OMA" id="GGCFNNY"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008483">
        <property type="term" value="F:transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009058">
        <property type="term" value="P:biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="SSF53383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aminotran_1_2" evidence="1">
        <location>
            <begin position="56"/>
            <end position="383"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF09390.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="404" mass="44403" checksum="46510D605C536ACC" modified="2006-05-30" version="1">MNSPTTRPIMKWAISRRAQQLTSSAIREILKVTERPEVISFAGGLPSPASFPVAAMEAAVARVFADNPQSALQYAATEGFMPLREFVAKRYNVDVERVMITTGSQQALDLIAKVMIDPGSPVLVETPSYLGALQAFSLFEPEFVSIPSDEKGLIPEALTPELTAGARFLYALPNFQNPTGRRLPLERRQELVKRAKELGVLLVEDDPYGELSYSGNQLPSLLSMNPDGVIYMGSFSKILAPGMRLGFVIAPPDLHFKLCQAKQASDLHTPTFTQRIAYETIKDGLLEQHIPTIRELYGKQCAFMLDALKRHMPAGVTWNAPEGGMFIWVELPEGLDSMKILEEAVRRNVAYVPGAPFYAGSPRKNTLRLAFVTVSAERIEQGVSILGEVFREAIAQAAPQARAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LKJ3</accession>
    <name>Q1LKJ3_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Transporter, LysE family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Rmet_2456</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09333.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517047.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2456"/>
    <dbReference type="EnsemblBacteria" id="ABF09333">
        <property type="protein sequence ID" value="ABF09333"/>
        <property type="gene ID" value="Rmet_2456"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2456"/>
    <dbReference type="eggNOG" id="COG1280">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_079569_2_3_4"/>
    <dbReference type="OMA" id="ATPGPNM"/>
    <dbReference type="OrthoDB" id="1440181at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006865">
        <property type="term" value="P:amino acid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001123">
        <property type="entry name" value="LeuE-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30086">
        <property type="entry name" value="PTHR30086"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01810">
        <property type="entry name" value="LysE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006324">
        <property type="entry name" value="LeuE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="6"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="40"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="65"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="116"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="144"/>
            <end position="169"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABF09333.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="205" mass="21553" checksum="D72570EFC36C3C9D" modified="2006-05-30" version="1">MTFEHWITFVLTSVILLIIPGPTILLVIGDALAHRGRSAYATVAGVACGDLTAFTLSMAGLGAVLAASATLFTALKWVGAAYLVYLGVQALRTANQARELEVAPQSGSGAQRFAKAWLVTALNPKSIVFFVAFVPQFMDAKLAFWSQAVVLIPTFVVLASANATVYALLARSLATRLTTRRAQANMQRAGGITLIGAGALIATRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="60" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LKR1</accession>
    <name>Q1LKR1_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Heme exporter protein D</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">ccmD</name>
        <name evidence="12" type="ordered locus">Rmet_2388</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Required for the export of heme to the periplasm for the biogenesis of c-type cytochromes.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell inner membrane</location>
            <topology evidence="3">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the CcmD/CycX/HelD family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09265.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_003149824.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2388"/>
    <dbReference type="EnsemblBacteria" id="ABF09265">
        <property type="protein sequence ID" value="ABF09265"/>
        <property type="gene ID" value="Rmet_2388"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2388"/>
    <dbReference type="eggNOG" id="COG3114">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_180892_2_0_4"/>
    <dbReference type="OMA" id="SHFAERR"/>
    <dbReference type="OrthoDB" id="2101555at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017004">
        <property type="term" value="P:cytochrome complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015886">
        <property type="term" value="P:heme transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007078">
        <property type="entry name" value="Haem_export_protD_CcmD"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03141">
        <property type="entry name" value="cytochro_ccmD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002442"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004377"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00016461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ABF09265.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="63" mass="7331" checksum="FFFAF63AA5A72546" modified="2006-05-30" version="1">MNWASVGEFLAMGGYGVYVWGSVLTTVVLLWTECRMLRRRRRAALWRIQSELLGKEARREATK</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-12-02" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LKS1</accession>
    <name>Q1LKS1_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Putative cytochrome c</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Rmet_2378</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09255.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2378"/>
    <dbReference type="EnsemblBacteria" id="ABF09255">
        <property type="protein sequence ID" value="ABF09255"/>
        <property type="gene ID" value="Rmet_2378"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2378"/>
    <dbReference type="eggNOG" id="COG3258">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_058582_1_0_4"/>
    <dbReference type="OMA" id="IYENMPF"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.760.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009056">
        <property type="entry name" value="Cyt_c-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036909">
        <property type="entry name" value="Cyt_c-like_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13442">
        <property type="entry name" value="Cytochrome_CBB3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46626">
        <property type="entry name" value="SSF46626"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51007">
        <property type="entry name" value="CYTC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 4" id="KW-0349">Heme</keyword>
    <keyword evidence="3 4" id="KW-0408">Iron</keyword>
    <keyword evidence="2 4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cytochrome c" evidence="5">
        <location>
            <begin position="24"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="domain" description="Cytochrome c" evidence="5">
        <location>
            <begin position="151"/>
            <end position="237"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABF09255.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="282" mass="30507" checksum="AC43D170F23BCB37" modified="2006-05-30" version="1">MPVGADGYYLPPAEDDIPKGPYGDAIRRGMKIFTDTGAMVKDHVGNSLACANCHLDSGRRENAAPMWAAYASYPAFRSKTGTISTLEDRIMGCFTYSMNAQASSSGKPPPAGSDVYRDLMTYMAWMADGLPAGNKPRGALYPKVPKPKDDYDVGRGLAVYQQNCALCHGPDGQGTREENGKMRFPPLWGPESYNWGAGMARIDTAAGFIWANMPLGKPYSLTEQEAWDVAAFINSHERPKDTRQTGTVAEALEKYHEGEQSYYGKVVGGKLLGVGTDAKSGP</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="61" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LL03</accession>
    <name>Q1LL03_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_2294</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09173.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_003116837.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2294"/>
    <dbReference type="EnsemblBacteria" id="ABF09173">
        <property type="protein sequence ID" value="ABF09173"/>
        <property type="gene ID" value="Rmet_2294"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2294"/>
    <dbReference type="eggNOG" id="ENOG502Z7Q1">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039028_2_1_4"/>
    <dbReference type="OMA" id="INEVRTV"/>
    <dbReference type="OrthoDB" id="359514at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="32"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="229"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="368"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="377"/>
            <end position="392"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF09173.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="412" mass="45118" checksum="1DF4FD7751FE540A" modified="2006-05-30" version="1">MAVDDTAPRAQRPGPIALADLFDAALKDLAPKSSSGAPASLPAPTPTPPTSGDAFLFSGNRHESVPRRLFLDRRLTPLERNAWQVFRLMLNDDGVTAFPTYEQLRPWLASMPCAGQASHETVARALTLLRLTRWLSLVRRRRDPKTGRILGNLYVLHDEPLTPFEAMQLDADYLALVSQSLGHSAKAVQVVGLNTLQEIADDPMLSGRTLPSRLQVLAERLADQGITASESYPQEDAVHDSEEGPASLLRNGDRPSSESEAGPKPAPDGALRNSKQDRTVRSSRIDEVRTTAQARALGDLQWPKRFAELKAEQQAGAKMALQQVDAALRQAVLDEWAARCGKHGIRNPAGYLFGIIQRAMRGEFNAWAKQTGPAPPTPSAARAPPTEPPRNVVPPEVARQHIERLRDLLRKS</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-04-29" modified="2022-08-03" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LL89</accession>
    <name>RNH_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Ribonuclease H</fullName>
            <shortName evidence="1">RNase H</shortName>
            <ecNumber evidence="1">3.1.26.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rnhA</name>
        <name type="ordered locus">Rmet_2208</name>
    </gene>
    <organism>
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Endonuclease that specifically degrades the RNA of RNA-DNA hybrids.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Endonucleolytic cleavage to 5'-phosphomonoester.</text>
            <dbReference type="EC" id="3.1.26.4"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="1">Binds 1 Mg(2+) ion per subunit. May bind a second metal ion at a regulatory site, or after substrate binding.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the RNase H family.</text>
    </comment>
    <dbReference type="EC" id="3.1.26.4" evidence="1"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09087.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516915.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q1LL89"/>
    <dbReference type="SMR" id="Q1LL89"/>
    <dbReference type="STRING" id="266264.Rmet_2208"/>
    <dbReference type="EnsemblBacteria" id="ABF09087">
        <property type="protein sequence ID" value="ABF09087"/>
        <property type="gene ID" value="Rmet_2208"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2208"/>
    <dbReference type="eggNOG" id="COG0328">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030894_6_0_4"/>
    <dbReference type="OMA" id="MQEIEIF"/>
    <dbReference type="OrthoDB" id="1953872at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004523">
        <property type="term" value="F:RNA-DNA hybrid ribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006401">
        <property type="term" value="P:RNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd09278">
        <property type="entry name" value="RNase_HI_prokaryote_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00042">
        <property type="entry name" value="RNase_H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002156">
        <property type="entry name" value="RNaseH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022892">
        <property type="entry name" value="RNaseHI"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00075">
        <property type="entry name" value="RNase_H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="SSF53098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50879">
        <property type="entry name" value="RNASE_H_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0255">Endonuclease</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0540">Nuclease</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000332660" description="Ribonuclease H">
        <location>
            <begin position="1"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="domain" description="RNase H type-1" evidence="2">
        <location>
            <begin position="1"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="9"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="9"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="47"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="69"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="133"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00408"/>
        </source>
    </evidence>
    <sequence length="145" mass="15966" checksum="0153403085748C65" modified="2006-05-30" version="1">MQEVTIYSDGACKGNPGPGGWGAVLVAGGHEKELFGGESPTTNNRMELMAVIEALRALKRPCIVNIYTDSQYVQKGISEWIHGWKARGWKTADKKPVKNADLWQALDEAQKPHQITWHWVRGHNGHPGNERADALANRGVASINT</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="63" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LLD1</accession>
    <name>Q1LLD1_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_2166</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09045.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516875.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2166"/>
    <dbReference type="EnsemblBacteria" id="ABF09045">
        <property type="protein sequence ID" value="ABF09045"/>
        <property type="gene ID" value="Rmet_2166"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2166"/>
    <dbReference type="eggNOG" id="COG0603">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_048552_0_0_4"/>
    <dbReference type="OMA" id="RAWDFLS"/>
    <dbReference type="OrthoDB" id="1861107at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF09045.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="419" mass="46141" checksum="CF75382128B0EC43" modified="2006-05-30" version="1">MKVICTSFDARPRAISAGTRAFTFFQSSDVAGSERIANGWLQELERAGYAPSIPIWDFVLFGFAVCAADLAVPRNKSADGWTRELELSVTVVDPTPWNAQRPLVEGMLRILTGDFWSVTFLSDGPQPPRGQRRICDRDCVALLSGGLDSLIGGIDAVAKMRRPIFVSQLAYEDSERQRQYAAALGGAAWHQQWSHRIDPAAPREPSTRARSMGFYALAVLATSLLTTTGPAEILVPENGFISVNPPLLPGRMASLSTRTTHPLFMKQLQGLLKAVGIPANLSMPYRFKTKGEMMMECLDQKLLAHFASDSTSCGRYRTYNRTHCGRCVPCMVRRAAFLRWGQADDTRYKFPNLRLSEKSAADDPMAVACAVLATEANGIDAFLGATLSFATQNERVHYRDVAERALLELSTLLKQDGVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LLE6</accession>
    <name>Q1LLE6_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Peptidoglycan glycosyltransferase (Penicillin-binding domain)</fullName>
            <ecNumber evidence="5">2.4.1.129</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">ftsI1</name>
        <name evidence="5" type="ordered locus">Rmet_2151</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.4.1.129" evidence="5"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09030.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516857.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2151"/>
    <dbReference type="EnsemblBacteria" id="ABF09030">
        <property type="protein sequence ID" value="ABF09030"/>
        <property type="gene ID" value="Rmet_2151"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2151"/>
    <dbReference type="eggNOG" id="COG0768">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009289_6_2_4"/>
    <dbReference type="OMA" id="IVMESKT"/>
    <dbReference type="OrthoDB" id="213680at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008658">
        <property type="term" value="F:penicillin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008955">
        <property type="term" value="F:peptidoglycan glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.710.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012338">
        <property type="entry name" value="Beta-lactam/transpept-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005311">
        <property type="entry name" value="PBP_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036138">
        <property type="entry name" value="PBP_dimer_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001460">
        <property type="entry name" value="PCN-bd_Tpept"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03717">
        <property type="entry name" value="PBP_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00905">
        <property type="entry name" value="Transpeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56519">
        <property type="entry name" value="SSF56519"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56601">
        <property type="entry name" value="SSF56601"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="1 2" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="43"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="domain" description="PBP_dimer" evidence="4">
        <location>
            <begin position="84"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="domain" description="Transpeptidase" evidence="3">
        <location>
            <begin position="271"/>
            <end position="559"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00905"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF03717"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABF09030.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="581" mass="62218" checksum="EEA7A096A984522C" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="101" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LLM3</accession>
    <name>Q1LLM3_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Nitrate reductase (quinone)</fullName>
            <ecNumber evidence="7">1.7.5.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">narG</name>
        <name evidence="14" type="ordered locus">Rmet_2074</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a quinol + nitrate = a quinone + H2O + nitrite</text>
            <dbReference type="Rhea" id="RHEA:56144"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16301"/>
            <dbReference type="ChEBI" id="CHEBI:17632"/>
            <dbReference type="ChEBI" id="CHEBI:24646"/>
            <dbReference type="ChEBI" id="CHEBI:132124"/>
            <dbReference type="EC" id="1.7.5.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mo-bis(molybdopterin guanine dinucleotide)</name>
            <dbReference type="ChEBI" id="CHEBI:60539"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cell membrane</location>
            <topology evidence="5">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Membrane</location>
            <topology evidence="4">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the prokaryotic molybdopterin-containing oxidoreductase family.</text>
    </comment>
    <dbReference type="EC" id="1.7.5.1" evidence="7"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08953.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516787.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2074"/>
    <dbReference type="EnsemblBacteria" id="ABF08953">
        <property type="protein sequence ID" value="ABF08953"/>
        <property type="gene ID" value="Rmet_2074"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2074"/>
    <dbReference type="eggNOG" id="COG5013">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000422_14_1_4"/>
    <dbReference type="OMA" id="GGHHNSV"/>
    <dbReference type="OrthoDB" id="88184at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009325">
        <property type="term" value="C:nitrate reductase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043546">
        <property type="term" value="F:molybdopterin cofactor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008940">
        <property type="term" value="F:nitrate reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042126">
        <property type="term" value="P:nitrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02776">
        <property type="entry name" value="MopB_CT_Nitrate-R-NarG-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1200.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009010">
        <property type="entry name" value="Asp_de-COase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037943">
        <property type="entry name" value="MopB_CT_Nitrate-R-NarG-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006657">
        <property type="entry name" value="MoPterin_dinucl-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006656">
        <property type="entry name" value="Mopterin_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006963">
        <property type="entry name" value="Mopterin_OxRdtase_4Fe-4S_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006655">
        <property type="entry name" value="Mopterin_OxRdtase_prok_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027467">
        <property type="entry name" value="MopterinOxRdtase_cofactor_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006468">
        <property type="entry name" value="NarG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028189">
        <property type="entry name" value="Nitr_red_alph_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044906">
        <property type="entry name" value="Nitr_red_alph_N_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00384">
        <property type="entry name" value="Molybdopterin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01568">
        <property type="entry name" value="Molydop_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14710">
        <property type="entry name" value="Nitr_red_alph_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00926">
        <property type="entry name" value="Molybdop_Fe4S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50692">
        <property type="entry name" value="SSF50692"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01580">
        <property type="entry name" value="narG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51669">
        <property type="entry name" value="4FE4S_MOW_BIS_MGD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00551">
        <property type="entry name" value="MOLYBDOPTERIN_PROK_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00932">
        <property type="entry name" value="MOLYBDOPTERIN_PROK_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="8" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10 12" id="KW-0408">Iron</keyword>
    <keyword evidence="11 12" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="9 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="4Fe-4S Mo/W bis-MGD-type" evidence="13">
        <location>
            <begin position="43"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="50"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="54"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="58"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="93"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001854"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00010312"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00012500"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51669"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ABF08953.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="1252" mass="140660" checksum="BB89167FFE64DF46" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="70" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LLQ0</accession>
    <name>Q1LLQ0_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">AFG1-like ATPase</fullName>
            <ecNumber evidence="3">3.6.1.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_2047</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EC" id="3.6.1.-" evidence="3"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08926.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516758.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2047"/>
    <dbReference type="EnsemblBacteria" id="ABF08926">
        <property type="protein sequence ID" value="ABF08926"/>
        <property type="gene ID" value="Rmet_2047"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2047"/>
    <dbReference type="eggNOG" id="COG1485">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008681_0_4_4"/>
    <dbReference type="OMA" id="FDEMQIT"/>
    <dbReference type="OrthoDB" id="607330at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005654">
        <property type="entry name" value="ATPase_AFG1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12169">
        <property type="entry name" value="PTHR12169"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03969">
        <property type="entry name" value="AFG1_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF08926.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="365" mass="42607" checksum="A21DA5036ABBBBDE" modified="2006-05-30" version="1">MNVTEFYEHELKQRGYQSDEAQRRAVERLQQCYDEWVAYKGRRNSTLKKLLVHPEVPKGVYMWGGVGRGKSFLMDSFYSCVPVVRKTRLHFHEFMREVHRELEELRGRADPLDELAKRIARRFRLICFDEFHVNDVADAMILHRLLQQLFENGVQFVMTSNYRPDLLYPDGLHRDRVLPAIALLQQKLDVLNVDAGIDYRKRALEQVQAYHTPLNREASAALRDAFVSIAGTADESPILHIEHREIKALRKANGVVWFDFATLCGGPRSQNDYLEIASQFHTVILSDVPKMTPRMSSEARRFTWLIDVFYDHKVKLLMSAEVPADELYTEGQMANEFHRTVSRIIEMQSREYLESERRTVDTSLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LLX7</accession>
    <name>Q1LLX7_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">PhoH family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Rmet_1970</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PhoH family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08849.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516694.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1970"/>
    <dbReference type="EnsemblBacteria" id="ABF08849">
        <property type="protein sequence ID" value="ABF08849"/>
        <property type="gene ID" value="Rmet_1970"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1970"/>
    <dbReference type="eggNOG" id="COG1875">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022283_0_1_4"/>
    <dbReference type="OMA" id="KMSPWAQ"/>
    <dbReference type="OrthoDB" id="445854at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003714">
        <property type="entry name" value="PhoH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029060">
        <property type="entry name" value="PIN-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002716">
        <property type="entry name" value="PIN_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02562">
        <property type="entry name" value="PhoH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13638">
        <property type="entry name" value="PIN_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00670">
        <property type="entry name" value="PINc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88723">
        <property type="entry name" value="SSF88723"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PINc" evidence="5">
        <location>
            <begin position="103"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="47"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="53"/>
            <end position="86"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010393"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABF08849.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="564" mass="61796" checksum="A78E7676C5F4B7D0" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-10-07" version="64" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LM37</accession>
    <name>Q1LM37_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_1910</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08789.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1910"/>
    <dbReference type="EnsemblBacteria" id="ABF08789">
        <property type="protein sequence ID" value="ABF08789"/>
        <property type="gene ID" value="Rmet_1910"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1910"/>
    <dbReference type="eggNOG" id="COG3246">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_065536_2_0_4"/>
    <dbReference type="OMA" id="FECFDVG"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019475">
        <property type="term" value="P:L-lysine catabolic process to acetate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008567">
        <property type="entry name" value="KCE_family"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37418">
        <property type="entry name" value="PTHR37418"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05853">
        <property type="entry name" value="BKACE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF08789.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="311" mass="33119" checksum="838ADCF21E2C55C7" modified="2006-05-30" version="1">MAGASSADSFSSQFNVSMHARKTIITCAVTGNIVKPDQHPGLPITPEQIANAALEAADAGAAVAHIHVRDPETGKPSMRLDYYADVIDRIRARNRALIINLTTGPGGRFVPDEDEPRVAAAGTTLLHPLKRVEHIAALRPDVCSLDLNTMNSGADVVINTPGNVRKMAEVIRAAGVMPELEIFDSGDLNMALDFMRDGVLEGPGLWTFVLGVKYGFAPTPETIFYARNMLPPGAHWSAFGIGRAEFPIVAQAWLAGGHVRVGLEDNIYLEKGVLAPSNAALVAKARDIVKSLGGDIASSADARRTLGLREV</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="66" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LM39</accession>
    <name>Q1LM39_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Cupin_2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_1908</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08787.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008651613.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1908"/>
    <dbReference type="EnsemblBacteria" id="ABF08787">
        <property type="protein sequence ID" value="ABF08787"/>
        <property type="gene ID" value="Rmet_1908"/>
    </dbReference>
    <dbReference type="GeneID" id="60821540"/>
    <dbReference type="KEGG" id="rme:Rmet_1908"/>
    <dbReference type="eggNOG" id="COG1917">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_168167_0_0_4"/>
    <dbReference type="OMA" id="EKHYVVL"/>
    <dbReference type="OrthoDB" id="1928636at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013096">
        <property type="entry name" value="Cupin_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011051">
        <property type="entry name" value="RmlC_Cupin_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07883">
        <property type="entry name" value="Cupin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51182">
        <property type="entry name" value="SSF51182"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cupin_2" evidence="1">
        <location>
            <begin position="41"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF07883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF08787.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="116" mass="12450" checksum="16E16BE85159F3D2" modified="2006-05-30" version="1">MNTTRFAQAPAYFPANHEGMHCLRLQGHEAGPSEALWMGVSVILPGGHTSMDASPVEKHYVVLEGEVCIRTPGESVTLGQFDSVRLAPGEGRQVSNPGNRPAMLLLAMPYPKAVNP</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LM71</accession>
    <name>Q1LM71_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">TRAP-type transporter, large permease component / DctM analogue</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Rmet_1876</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell inner membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08755.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516601.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1876"/>
    <dbReference type="EnsemblBacteria" id="ABF08755">
        <property type="protein sequence ID" value="ABF08755"/>
        <property type="gene ID" value="Rmet_1876"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1876"/>
    <dbReference type="eggNOG" id="COG1593">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019824_4_0_4"/>
    <dbReference type="OMA" id="NCFIVAR"/>
    <dbReference type="OrthoDB" id="903568at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010656">
        <property type="entry name" value="DctM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004681">
        <property type="entry name" value="TRAP_DctM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33362">
        <property type="entry name" value="PTHR33362"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06808">
        <property type="entry name" value="DctM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006066">
        <property type="entry name" value="HI0050"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00786">
        <property type="entry name" value="dctM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="53"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="142"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="172"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="220"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="247"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="277"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="310"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="339"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="362"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="397"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="domain" description="DctM" evidence="4">
        <location>
            <begin position="11"/>
            <end position="421"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004429"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF06808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABF08755.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="436" mass="46950" checksum="28BEDB359BA8B508" modified="2006-05-30" version="1">MSTVLVALILLLVMIAFLAIGAWIPVAIAVTSWVGLVIFSDHDALVNLANSWWSSSASYTLASLPLFVWMGEILFRTKLSEQMFNGLSPWLNWLPGRLMHVNILGCGIFGSVSGSSAATCATIAKSALPELTRRGYDERITLGSLSTAGTLGILIPPSITMVVYAVSADVSIIRVFLAGFLPGLLLMILFSGYIVVWALMNPEKTPAAETFGWSARLASIRQLMPCIILIAFITWIMMAGYSTATEAAAYGVVASLALAWAGGSLTRKAFWESLMSATRLTAMIMFVLGATSFLSVTMSFTGIPRALAEWVAALHLSPWALIAVLTIIYILLGTALDGISMIALTTATVLPMVQAAGFDLVWFGIFIVLLVEIAEVTPPVGFNLFVLQSMTGKDSNYIARVSLPFFMMMVLAIGIITVWPSVVTWLPDVVMAKELK</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="63" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LMI3</accession>
    <name>Q1LMI3_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_1764</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08643.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516496.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1764"/>
    <dbReference type="EnsemblBacteria" id="ABF08643">
        <property type="protein sequence ID" value="ABF08643"/>
        <property type="gene ID" value="Rmet_1764"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1764"/>
    <dbReference type="eggNOG" id="COG4188">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045366_0_0_4"/>
    <dbReference type="OMA" id="HIWAGEK"/>
    <dbReference type="OrthoDB" id="685252at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016986">
        <property type="entry name" value="UCP031982_abhydr"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF031982">
        <property type="entry name" value="UCP031982_abhydr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="SSF53474"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193318" evidence="1">
        <location>
            <begin position="21"/>
            <end position="340"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF08643.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="340" mass="36447" checksum="CE3387072ADCF391" modified="2006-05-30" version="1">MHRFRWAGLLLALLCGIAHASMGLTELPASGDDGPVTVYYPSNDASHPVKRGRFLLDVAVEGHPVAGNGRLIVISHGSGGAPWVHADLARNLVDAGYIVAMPLHKADNYLDSSDPGPDSWTIRPAEVSRAIDAMGRDARFASMLRLDKVGMFGISAGGHTALSLAGGRWSPARFMQHCDAHIAEDFQTCVGLITRLTGGPLDGVKEWLALRVIHHRFDDDTPRATFDPRIAAVVAGVPAAADFDMDSLVRPAVPLGLVTAEQDRWLIPRFHADRVLAVCQTCEHVADMPTGGHGALLSPHPPGLTGLLGDLLNDPPGFDRAEVAEVHRKIVAFFGRHLLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-10-07" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LMQ6</accession>
    <name>Q1LMQ6_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">EAL domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_1689</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08570.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1689"/>
    <dbReference type="EnsemblBacteria" id="ABF08570">
        <property type="protein sequence ID" value="ABF08570"/>
        <property type="gene ID" value="Rmet_1689"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1689"/>
    <dbReference type="eggNOG" id="COG2200">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_70_50_4"/>
    <dbReference type="OMA" id="FYQPIIG"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd01948">
        <property type="entry name" value="EAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.450">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001633">
        <property type="entry name" value="EAL_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035919">
        <property type="entry name" value="EAL_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00563">
        <property type="entry name" value="EAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00052">
        <property type="entry name" value="EAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF141868">
        <property type="entry name" value="SSF141868"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50883">
        <property type="entry name" value="EAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="EAL" evidence="1">
        <location>
            <begin position="1"/>
            <end position="235"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF08570.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="344" mass="37648" checksum="FBC22D915D58C4EF" modified="2006-05-30" version="1">MMYQPILDLRTGGILKIEALARLLDDEDVLLPASFLPHLDPEHLLELYVLGLDQALQEQRNWLDHGKAIGFAINVPPDGLDDPRYYDATVKALARHDRAPQLLTLEILESEGLSDTNEALTQFNRFRTLGVCMAEDDLGYGHSSLIRLRRLPFDCIKIDRDIVAMSTVDDVDALHFVYQLTRLGHALGKTVIVEGVDSVGLIDAIGTLGADGAQGYAVARPMPARQLSDWLENRQGPPSSVPSSSAPGRLAHLVVAEERLLAGGQDFPVVDLRCAGLAHDSAYTCMAQTCRTCRLARFRDEVHTTFGPLTNVGSEVNELLQAATRDGVSALAYRTARRKLAAAM</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LMT7</accession>
    <name>Q1LMT7_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">S-transferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">yqjG</name>
        <name evidence="3" type="ordered locus">Rmet_1656</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08539.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516385.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1656"/>
    <dbReference type="EnsemblBacteria" id="ABF08539">
        <property type="protein sequence ID" value="ABF08539"/>
        <property type="gene ID" value="Rmet_1656"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1656"/>
    <dbReference type="eggNOG" id="COG0435">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037263_0_1_4"/>
    <dbReference type="OMA" id="PWANRAI"/>
    <dbReference type="OrthoDB" id="780709at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004364">
        <property type="term" value="F:glutathione transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006749">
        <property type="term" value="P:glutathione metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010987">
        <property type="entry name" value="Glutathione-S-Trfase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036282">
        <property type="entry name" value="Glutathione-S-Trfase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040079">
        <property type="entry name" value="Glutathione_S-Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004045">
        <property type="entry name" value="Glutathione_S-Trfase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016639">
        <property type="entry name" value="GST_Omega/GSH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32419">
        <property type="entry name" value="PTHR32419"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13409">
        <property type="entry name" value="GST_N_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015753">
        <property type="entry name" value="GST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00019">
        <property type="entry name" value="Glutathione_Transferase_(cytos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01206">
        <property type="entry name" value="Xi.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47616">
        <property type="entry name" value="SSF47616"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="SSF52833"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50405">
        <property type="entry name" value="GST_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="GST C-terminal" evidence="2">
        <location>
            <begin position="171"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="1">
        <location>
            <position position="63"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="1">
        <location>
            <position position="194"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRSR" id="PIRSR015753-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF08539.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="328" mass="37163" checksum="93013D8FCF16FF41" modified="2006-05-30" version="1">MGLLVNGEWQDRWYDTTSTGGRFVRKNAAFRNWVTCDGAAGPTGIGGFRAEVGRYHLYVSLACPWAHRTLIMRELKGLEDMISFSVVHWLMREEGWTFADGPGVIPDSVSGARVLHQVYTLANPNYTGRVTVPVLWDRQQATIVSNESSEIIRMMNSAFDRLGARLGDYYPLDLRAQIDAINARIYDTVNNGVYKAGFATSQAAYEESVRSLFDTLDWLEAQLSQQRWLVGNRFTEADIRLFTTLIRFDAVYVGHFKCNLRRIVDYPALSAYLRDIYQRPGIAGTVDFLHIKGHYYESHRMLNPTGIVPLGPMLDLSSPHGRERLGQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LN36</accession>
    <name>Q1LN36_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Conjugal transfer protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">trbF</name>
        <name evidence="7" type="ordered locus">Rmet_1557</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08440.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516294.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1557"/>
    <dbReference type="EnsemblBacteria" id="ABF08440">
        <property type="protein sequence ID" value="ABF08440"/>
        <property type="gene ID" value="Rmet_1557"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1557"/>
    <dbReference type="eggNOG" id="COG3701">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_076026_0_0_4"/>
    <dbReference type="OMA" id="FVNALNW"/>
    <dbReference type="OrthoDB" id="1214981at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd16425">
        <property type="entry name" value="TrbF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032710">
        <property type="entry name" value="NTF2-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035658">
        <property type="entry name" value="TrbF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007430">
        <property type="entry name" value="VirB8"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04335">
        <property type="entry name" value="VirB8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54427">
        <property type="entry name" value="SSF54427"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="43"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="domain" description="VirB8" evidence="6">
        <location>
            <begin position="23"/>
            <end position="225"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF04335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABF08440.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="234" mass="26052" checksum="58450F31B4AD4DB9" modified="2006-05-30" version="1">MRFKKPQVRYADTPQAATPYQAAGQVWDERIGSPRVQAKNWRLMAFGCLTLALLMAGGLVWRSAQSIVTPYVVEVDNAGQVRAVGEAATPYRPNDAQTAHHIARFVTLVRSLSIDPIVVRQNWLDAYDYTTDKGAVALNDYARVNDPFARIGKESVTVQIASVTRASDTSFNVRWTERRYVNGAAAGTERWNAVISIVQQTPRTEERLRKNPLGIYVNGLSWSRELDSSEGAKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="72" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LN60</accession>
    <name>Q1LN60_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Nickel/cobalt efflux system</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">hoxN</name>
        <name evidence="9" type="ordered locus">Rmet_1533</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the NiCoT transporter (TC 2.A.52) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08416.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516273.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1533"/>
    <dbReference type="EnsemblBacteria" id="ABF08416">
        <property type="protein sequence ID" value="ABF08416"/>
        <property type="gene ID" value="Rmet_1533"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1533"/>
    <dbReference type="eggNOG" id="COG3376">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_090283_0_0_4"/>
    <dbReference type="OMA" id="GINGLWI"/>
    <dbReference type="OrthoDB" id="1515392at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005887">
        <property type="term" value="C:integral component of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015099">
        <property type="term" value="F:nickel cation transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004688">
        <property type="entry name" value="Ni/Co_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011541">
        <property type="entry name" value="Ni/Co_transpt_high_affinity"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31611">
        <property type="entry name" value="PTHR31611"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03824">
        <property type="entry name" value="NicO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0533">Nickel</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 8" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="45"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="87"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="127"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="167"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="211"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="242"/>
            <end position="262"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022596"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU362101"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ABF08416.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="278" mass="29393" checksum="0A37EF86746F7F06" modified="2006-05-30" version="1">MEALPNDWVALAALVFMLGVKHGFDADHLATIDGLTRFNLRHGAPVARACGILFSLGHGTVVIAIALAVSTLARRWQVPGWLEASGAWISIGFLVALGVINLRAVLTTRHDEVVQPVGLKGRWLGRLQHASHPLAVAAVGALFALSFDTVSQAALFAAAGAQYGGWGHALALGLLFTLGMLVMDGINGLWISRLIRRTDQMALVASRVMSLAVSGTSLLVAAFGVMRQLLPAVDAWSDGKELAFGLSVVCVIAGSFFLALRLTRVPAVAAARPMRFVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LND5</accession>
    <name>Q1LND5_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">UPF0125 protein Rmet_1458</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">yfjF</name>
        <name evidence="4" type="ordered locus">Rmet_1458</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the UPF0125 (RnfH) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08341.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516207.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="SMR" id="Q1LND5"/>
    <dbReference type="STRING" id="266264.Rmet_1458"/>
    <dbReference type="EnsemblBacteria" id="ABF08341">
        <property type="protein sequence ID" value="ABF08341"/>
        <property type="gene ID" value="Rmet_1458"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1458"/>
    <dbReference type="eggNOG" id="COG2914">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_150721_0_0_4"/>
    <dbReference type="OMA" id="DMIHVEV"/>
    <dbReference type="OrthoDB" id="1921802at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.280">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00460">
        <property type="entry name" value="UPF0125_RnfH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016155">
        <property type="entry name" value="Mopterin_synth/thiamin_S_b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005346">
        <property type="entry name" value="RnfH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037021">
        <property type="entry name" value="RnfH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37483">
        <property type="entry name" value="PTHR37483"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03658">
        <property type="entry name" value="Ub-RnfH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54285">
        <property type="entry name" value="SSF54285"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="94"/>
            <end position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF08341.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="121" mass="13023" checksum="BB36035A490BEB17" modified="2006-05-30" version="1">MPTTPTTAEVGGLVRLSVCYARPDTVFLKELDVPAGTTIIGVIEASGLTQACPEVDPSTLRVGVYGKLKTLDTPVRQGDRVEVYRVLTADPKTARRKRVQRTRASGTREGQKWLRGGGQGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="63" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LND9</accession>
    <name>Q1LND9_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">NfeD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_1454</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08337.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516203.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1454"/>
    <dbReference type="EnsemblBacteria" id="ABF08337">
        <property type="protein sequence ID" value="ABF08337"/>
        <property type="gene ID" value="Rmet_1454"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1454"/>
    <dbReference type="eggNOG" id="COG1585">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_116732_0_0_4"/>
    <dbReference type="OMA" id="PYRGADW"/>
    <dbReference type="OrthoDB" id="1951939at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002810">
        <property type="entry name" value="NfeD-like_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01957">
        <property type="entry name" value="NfeD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="47"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="domain" description="NfeD" evidence="2">
        <location>
            <begin position="53"/>
            <end position="140"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01957"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF08337.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="144" mass="15589" checksum="9247EDDDF52A8E2C" modified="2006-05-30" version="1">MTSYYLWFAVAVVLVIAELATGTFYLLMIALGVAAGGVAALLGADEPIQTVAAALVAIIGIVLLRRTRFGKLRRRDAASNPDVNLDIGQELEVPAWDDSRRARVPYRGADWTVELAPGAEPKPGRYRIVEVRGATLIVARHYTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LNH8</accession>
    <name>Q1LNH8_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Aldehyde oxidase</fullName>
            <ecNumber evidence="2">1.17.1.4</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_1415</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EC" id="1.17.1.4" evidence="2"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08298.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516170.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1415"/>
    <dbReference type="EnsemblBacteria" id="ABF08298">
        <property type="protein sequence ID" value="ABF08298"/>
        <property type="gene ID" value="Rmet_1415"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1415"/>
    <dbReference type="eggNOG" id="COG1529">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_311632_0_0_4"/>
    <dbReference type="OMA" id="ANDRYEQ"/>
    <dbReference type="OrthoDB" id="117507at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004854">
        <property type="term" value="F:xanthine dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036856">
        <property type="entry name" value="Ald_Oxase/Xan_DH_a/b_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016208">
        <property type="entry name" value="Ald_Oxase/xanthine_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008274">
        <property type="entry name" value="AldOxase/xan_DH_Mopterin-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037165">
        <property type="entry name" value="AldOxase/xan_DH_Mopterin-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006311">
        <property type="entry name" value="TAT_signal"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11908">
        <property type="entry name" value="PTHR11908"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02738">
        <property type="entry name" value="Ald_Xan_dh_C2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54665">
        <property type="entry name" value="SSF54665"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56003">
        <property type="entry name" value="SSF56003"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51318">
        <property type="entry name" value="TAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ald_Xan_dh_C2" evidence="1">
        <location>
            <begin position="265"/>
            <end position="604"/>
        </location>
    </feature>
    <feature type="domain" description="Ald_Xan_dh_C2" evidence="1">
        <location>
            <begin position="689"/>
            <end position="880"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF08298.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="941" mass="102715" checksum="1AA73F5ACE440B56" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LNJ9</accession>
    <name>Q1LNJ9_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Acyl-CoA-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_1394</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08277.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008649110.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1394"/>
    <dbReference type="EnsemblBacteria" id="ABF08277">
        <property type="protein sequence ID" value="ABF08277"/>
        <property type="gene ID" value="Rmet_1394"/>
    </dbReference>
    <dbReference type="GeneID" id="60822150"/>
    <dbReference type="KEGG" id="rme:Rmet_1394"/>
    <dbReference type="eggNOG" id="COG4281">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_118853_4_1_4"/>
    <dbReference type="OMA" id="RYKFEAW"/>
    <dbReference type="OrthoDB" id="1980628at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000062">
        <property type="term" value="F:fatty-acyl-CoA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.80.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000582">
        <property type="entry name" value="Acyl-CoA-binding_protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035984">
        <property type="entry name" value="Acyl-CoA-binding_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014352">
        <property type="entry name" value="FERM/acyl-CoA-bd_prot_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00887">
        <property type="entry name" value="ACBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00689">
        <property type="entry name" value="ACOABINDINGP"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47027">
        <property type="entry name" value="SSF47027"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51228">
        <property type="entry name" value="ACB_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ACB" evidence="1">
        <location>
            <begin position="4"/>
            <end position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF08277.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="89" mass="10088" checksum="A398D2A8A509E07F" modified="2006-05-30" version="1">MSDLQTRFDQAQIDVKTLSERPNNMTLLRLYALYKQGAEGDAHGDKPGMTDFVGRYKFEAWEALKGTGREEAMQKYIELVTDLLEGKAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="97" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LNL3</accession>
    <name>Q1LNL3_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Histidine kinase</fullName>
            <ecNumber evidence="2">2.7.13.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Rmet_1380</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.</text>
            <dbReference type="EC" id="2.7.13.3"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.7.13.3" evidence="2"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08263.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516141.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1380"/>
    <dbReference type="EnsemblBacteria" id="ABF08263">
        <property type="protein sequence ID" value="ABF08263"/>
        <property type="gene ID" value="Rmet_1380"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1380"/>
    <dbReference type="eggNOG" id="COG2972">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020473_1_1_4"/>
    <dbReference type="OMA" id="TQLEPHM"/>
    <dbReference type="OrthoDB" id="1031920at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004358">
        <property type="entry name" value="Sig_transdc_His_kin-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010559">
        <property type="entry name" value="Sig_transdc_His_kin_internal"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06580">
        <property type="entry name" value="His_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00344">
        <property type="entry name" value="BCTRLSENSOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="SSF55874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="18"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="48"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="76"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="114"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="5">
        <location>
            <begin position="261"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="144"/>
            <end position="173"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABF08263.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="390" mass="42879" checksum="B04BFFDF4C536BC4" modified="2006-05-30" version="1">MRLSCAGPSRLNILWRDLLFVLCMNTVIAVSLNYGFRTAGTLLQNFAYSQLIGLSIWVLIDIPRVLIWWNDRPRRWPFMCLAVVAVPMGVVMGSWATRELLGLPEKSAAETGDMLRMCLIVGMLASASMIYFYWSREKLAYLERQAALDALQREEAEKQLVRAQLMALQAQIEPHFLFNSLANLDGLIATDPRAARQLLQRLIGFLRMSLSHTRAEHCTLAQEFELLRSYLDIQGMRFGARLDYEIDLPPALAEAEIPPMLVQPLVENAVTHGIEPCMRGGKIVLSARETGDGSLQVVIADTGVGFSQGNKGSKGGSGLGLTHVRERLARIFGAAATMQMEENTPRGVVVRLTLPLQRGFEPMSVPVRAAAGAADLQHAPAGVAPAVSRS</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2007-03-06" modified="2022-08-03" version="100" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LNM0</accession>
    <name>SSUB_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Aliphatic sulfonates import ATP-binding protein SsuB</fullName>
            <ecNumber evidence="1">7.6.2.14</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">ssuB</name>
        <name type="ordered locus">Rmet_1373</name>
    </gene>
    <organism>
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Part of the ABC transporter complex SsuABC involved in aliphatic sulfonates import. Responsible for energy coupling to the transport system.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + H2O + aliphatic sulfonate-[sulfonate-binding protein]Side 1 = ADP + phosphate + aliphatic sulfonateSide 2 + [sulfonate-binding protein]Side 1.</text>
            <dbReference type="EC" id="7.6.2.14"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">The complex is composed of two ATP-binding proteins (SsuB), two transmembrane proteins (SsuC) and a solute-binding protein (SsuA).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell inner membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the ABC transporter superfamily. Aliphatic sulfonates importer (TC 3.A.1.17.2) family.</text>
    </comment>
    <dbReference type="EC" id="7.6.2.14" evidence="1"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08256.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516134.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q1LNM0"/>
    <dbReference type="SMR" id="Q1LNM0"/>
    <dbReference type="STRING" id="266264.Rmet_1373"/>
    <dbReference type="EnsemblBacteria" id="ABF08256">
        <property type="protein sequence ID" value="ABF08256"/>
        <property type="gene ID" value="Rmet_1373"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1373"/>
    <dbReference type="eggNOG" id="COG1116">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_22_4"/>
    <dbReference type="OMA" id="ADRANDW"/>
    <dbReference type="OrthoDB" id="1200451at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51291">
        <property type="entry name" value="SSUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0997">Cell inner membrane</keyword>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-1278">Translocase</keyword>
    <keyword id="KW-0813">Transport</keyword>
    <feature type="chain" id="PRO_0000279948" description="Aliphatic sulfonates import ATP-binding protein SsuB">
        <location>
            <begin position="1"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="1">
        <location>
            <begin position="40"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="72"/>
            <end position="79"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01724"/>
        </source>
    </evidence>
    <sequence length="291" mass="31610" checksum="87F4C95FF66F1F41" modified="2006-05-30" version="1">MMHTHPVEQAALAWLDANLARRDAARPAARASHGVGGIALHVEQVVKRYNGREVLHGVNLRAAPGEFVAIVGRSGCGKSTLLRLVAGLESADAGAVALDGQPGGTPSSVRVMFQDARLLPWKRVLDNVAIGLPRERHADAAALLDQVGLADRANEWPARLSGGQRQRVALARALVHHPRLLLLDEPLGALDALTRIEMQALIESLWRRLGFTALLVTHDVSEAIALADRVVLIEDGRITLDQQVTLARPRQRGTADFAQIEDTVLRRVMRQESPAASDAELHFRSAEEFLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LNZ2</accession>
    <name>Q1LNZ2_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transcription regulator (Helix turn helix motif) XRE family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_1249</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08134.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516021.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1249"/>
    <dbReference type="EnsemblBacteria" id="ABF08134">
        <property type="protein sequence ID" value="ABF08134"/>
        <property type="gene ID" value="Rmet_1249"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1249"/>
    <dbReference type="eggNOG" id="COG1396">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_066192_35_2_4"/>
    <dbReference type="OMA" id="CEVMDVH"/>
    <dbReference type="OrthoDB" id="2063007at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00093">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001387">
        <property type="entry name" value="Cro/C1-type_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010982">
        <property type="entry name" value="Lambda_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01381">
        <property type="entry name" value="HTH_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00530">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47413">
        <property type="entry name" value="SSF47413"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50943">
        <property type="entry name" value="HTH_CROC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH cro/C1-type" evidence="1">
        <location>
            <begin position="11"/>
            <end position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF08134.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="101" mass="11145" checksum="C3907BD61A44CDED" modified="2006-05-30" version="1">MAAGNSLAKALKTVRKARGLSQEAFSDVSSRTYMSTLERDLKSPTLHKLAELCEVMDIHPLTLLTLAYAGDSPHKADELLVQVRRELEAVLKERDAAKPRA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-05-20" modified="2022-08-03" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LP27</accession>
    <name>PYRD_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Dihydroorotate dehydrogenase (quinone)</fullName>
            <ecNumber evidence="1">1.3.5.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">DHOdehase</fullName>
            <shortName evidence="1">DHOD</shortName>
            <shortName evidence="1">DHODase</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Dihydroorotate oxidase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">pyrD</name>
        <name type="ordered locus">Rmet_1213</name>
    </gene>
    <organism>
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the conversion of dihydroorotate to orotate with quinone as electron acceptor.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(S)-dihydroorotate + a quinone = a quinol + orotate</text>
            <dbReference type="Rhea" id="RHEA:30187"/>
            <dbReference type="ChEBI" id="CHEBI:24646"/>
            <dbReference type="ChEBI" id="CHEBI:30839"/>
            <dbReference type="ChEBI" id="CHEBI:30864"/>
            <dbReference type="ChEBI" id="CHEBI:132124"/>
            <dbReference type="EC" id="1.3.5.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
        <text evidence="1">Binds 1 FMN per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Pyrimidine metabolism; UMP biosynthesis via de novo pathway; orotate from (S)-dihydroorotate (quinone route): step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the dihydroorotate dehydrogenase family. Type 2 subfamily.</text>
    </comment>
    <comment type="sequence caution" evidence="2">
        <conflict type="erroneous initiation">
            <sequence resource="EMBL-CDS" id="ABF08099" version="1"/>
        </conflict>
    </comment>
    <dbReference type="EC" id="1.3.5.2" evidence="1"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08099.1"/>
        <property type="status" value="ALT_INIT"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_017514992.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q1LP27"/>
    <dbReference type="SMR" id="Q1LP27"/>
    <dbReference type="STRING" id="266264.Rmet_1213"/>
    <dbReference type="EnsemblBacteria" id="ABF08099">
        <property type="protein sequence ID" value="ABF08099"/>
        <property type="gene ID" value="Rmet_1213"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1213"/>
    <dbReference type="eggNOG" id="COG0167">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013640_2_0_4"/>
    <dbReference type="OrthoDB" id="1109542at2"/>
    <dbReference type="UniPathway" id="UPA00070">
        <property type="reaction ID" value="UER00946"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004152">
        <property type="term" value="F:dihydroorotate dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006207">
        <property type="term" value="P:'de novo' pyrimidine nucleobase biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044205">
        <property type="term" value="P:'de novo' UMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd04738">
        <property type="entry name" value="DHOD_2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00225">
        <property type="entry name" value="DHO_dh_type2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005720">
        <property type="entry name" value="Dihydroorotate_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012135">
        <property type="entry name" value="Dihydroorotate_DH_1_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005719">
        <property type="entry name" value="Dihydroorotate_DH_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001295">
        <property type="entry name" value="Dihydroorotate_DH_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01180">
        <property type="entry name" value="DHO_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000164">
        <property type="entry name" value="DHO_oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01036">
        <property type="entry name" value="pyrD_sub2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00911">
        <property type="entry name" value="DHODEHASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00912">
        <property type="entry name" value="DHODEHASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0285">Flavoprotein</keyword>
    <keyword id="KW-0288">FMN</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-0560">Oxidoreductase</keyword>
    <keyword id="KW-0665">Pyrimidine biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000336483" description="Dihydroorotate dehydrogenase (quinone)">
        <location>
            <begin position="1"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="1">
        <location>
            <position position="181"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="65"/>
            <end position="69"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="69"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="89"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="114"/>
            <end position="118"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="145"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="178"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="178"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="183"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="223"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="251"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="252"/>
            <end position="253"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="274"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="303"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="324"/>
            <end position="325"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="344" mass="36977" checksum="C3E49ACB89F75F11" modified="2008-05-20" version="2">MLNALYPLLRPALFSMDAEDAHHFTLNNLMRAKRMGLAGCIGNSIADDPRTVMGVRFPNPVGLAAGLDKDGAYIDGLAAFGFGFIEVGTVTPRAQPGNPRPRMFRLPQADALINRMGFNNGGVDAFIRNVQASRWKAEGGVLGLNIGKNADTPIERAADDYLYCLERVYPHASYVTVNISSPNTKNLRQLQGASELDSLLSTLKAAQQRLADQHKRYVPVALKIAPDLDDDQVRNIGDALVRHKIDGVIATNTTISRDAVKGLPHAEEAGGLSGRPVFEASTRVVRALREVIGDALPIIGVGGIFSGADARAKIDAGAQLVQVYSGLIYRGPTLVRECASALRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LPB3</accession>
    <name>Q1LPB3_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_1127</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08013.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515914.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1127"/>
    <dbReference type="EnsemblBacteria" id="ABF08013">
        <property type="protein sequence ID" value="ABF08013"/>
        <property type="gene ID" value="Rmet_1127"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1127"/>
    <dbReference type="eggNOG" id="COG1321">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_150493_1_0_4"/>
    <dbReference type="OMA" id="QLWRAHR"/>
    <dbReference type="OrthoDB" id="1944083at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF08013.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="97" mass="10376" checksum="5754E718FCDAE337" modified="2006-05-30" version="1">MSDDVNEQNGIDPVLEAILRALWEHAREAGGDLSLARLSKRAEAQMSVLRRVLTQLSDAGLAVMTLEEDGRGAARLTEEGEALCGALFEGDDGGMPH</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="66" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LPH1</accession>
    <name>Q1LPH1_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative lipoprotein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_1069</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07955.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515856.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1069"/>
    <dbReference type="EnsemblBacteria" id="ABF07955">
        <property type="protein sequence ID" value="ABF07955"/>
        <property type="gene ID" value="Rmet_1069"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1069"/>
    <dbReference type="eggNOG" id="COG3317">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_056157_0_0_4"/>
    <dbReference type="OMA" id="DSMQELK"/>
    <dbReference type="OrthoDB" id="1197067at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.310.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042268">
        <property type="entry name" value="BamC_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010653">
        <property type="entry name" value="NlpB/DapX"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06804">
        <property type="entry name" value="Lipoprotein_18"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193679" evidence="2">
        <location>
            <begin position="38"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="68"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="75"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF07955.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="413" mass="45169" checksum="171309F770BD2556" modified="2006-05-30" version="1">MKQKLNRRGATQVRRVAVVAPVLALSLIAGCSSINEAMQPDKIDYKSQGKKTASLDIPPDLTKLEGDRRYTVPDAAGTSTLSNYSQANKTREASPTDNVLPSTDGIRMERDGNQRWLVINNGMPPDKLWESLRGFWQENGFLLTQDSPETGIMETDWAENRAKIPQDIIRNTIGKVFDGLYSTSERDKFRTRVERSQNGQLEVFISHRGAQEQLTGVDKSQTVWTPRPADPELEAEFLSRLAQRLGVQKEQADKMAKNPTPAGIGTAQPSAAAAGVGAAAATGAATAAATSSSKSFLSQVNGSPALQLPEPFDRAWRSVGLSLDRVNFTVEDRDRAQGLYYVRYVDPKTTVDDRGFFGKLFTKPNDGKTAKKYRVALKGNGTGTTVTVLNDAGQPENSEIGKKILTLLDEQLH</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="96" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LQ06</accession>
    <name>Q1LQ06_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Transcriptional regulator, GntR-family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Rmet_0884</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07770.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515710.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0884"/>
    <dbReference type="EnsemblBacteria" id="ABF07770">
        <property type="protein sequence ID" value="ABF07770"/>
        <property type="gene ID" value="Rmet_0884"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0884"/>
    <dbReference type="eggNOG" id="COG1802">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017584_5_0_4"/>
    <dbReference type="OMA" id="ACSFDEH"/>
    <dbReference type="OrthoDB" id="1911275at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07377">
        <property type="entry name" value="WHTH_GntR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.530">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011711">
        <property type="entry name" value="GntR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008920">
        <property type="entry name" value="TF_FadR/GntR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000524">
        <property type="entry name" value="Tscrpt_reg_HTH_GntR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07729">
        <property type="entry name" value="FCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00392">
        <property type="entry name" value="GntR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00895">
        <property type="entry name" value="FCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00345">
        <property type="entry name" value="HTH_GNTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48008">
        <property type="entry name" value="SSF48008"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50949">
        <property type="entry name" value="HTH_GNTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH gntR-type" evidence="4">
        <location>
            <begin position="26"/>
            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABF07770.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="247" mass="27681" checksum="F5509CF12429B198" modified="2006-05-30" version="1">MSKNLKLIAAADALPALTDNKPARKGSVEERMYHEIYDAIMEHRLPPRTKLTEHSLCEIYATARHTVRKVLSRLAADGMVDLEPNRGAFIASPSTDEAHDMFELRQMLERAVLDKLASLPNVRTVIAPLRKMVNDERQAFLTHERPRWIRLSAEFHTALAELSGNQLLVTMMRRLVSRTTLMIASVESPGHNACSFDEHDEILDALEQGDAALAQTRMAHHLGACAERVQPDEPGNFDLRSVLGRST</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LQG5</accession>
    <name>Q1LQG5_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Lipopolysaccharide assembly protein B</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">lapB</name>
        <name evidence="4" type="ordered locus">Rmet_0725</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Modulates cellular lipopolysaccharide (LPS) levels by regulating LpxC, which is involved in lipid A biosynthesis. May act by modulating the proteolytic activity of FtsH towards LpxC. May also coordinate assembly of proteins involved in LPS synthesis at the plasma membrane.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell inner membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
            <orientation evidence="2">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the LapB family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07611.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515572.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0725"/>
    <dbReference type="EnsemblBacteria" id="ABF07611">
        <property type="protein sequence ID" value="ABF07611"/>
        <property type="gene ID" value="Rmet_0725"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0725"/>
    <dbReference type="eggNOG" id="COG2956">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_059365_1_0_4"/>
    <dbReference type="OMA" id="FWQCPGC"/>
    <dbReference type="OrthoDB" id="1425742at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031235">
        <property type="term" value="C:intrinsic component of the cytoplasmic side of the plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008653">
        <property type="term" value="P:lipopolysaccharide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046890">
        <property type="term" value="P:regulation of lipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00994">
        <property type="entry name" value="LPS_assembly_LapB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030865">
        <property type="entry name" value="LapB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041166">
        <property type="entry name" value="Rubredoxin_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_repeat"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18073">
        <property type="entry name" value="Rubredoxin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="SSF48452"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="2" id="KW-0408">Iron</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="1 2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0802">TPR repeat</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="topological domain" description="Cytoplasmic" evidence="2">
        <location>
            <begin position="23"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="domain" description="Rubredoxin_2" evidence="3">
        <location>
            <begin position="375"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="377"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="380"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="391"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="394"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF18073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF07611.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="409" mass="46048" checksum="B623275690ABEB7A" modified="2006-05-30" version="1">MMFETWWLLALPLVFGLGWMAARFDLRQLISEQGALPRSYFKGLNFLLNEQPDQAIDAFVEVARLDPETTELHFALGALFRRRGETERAIRVHQNLATRPDLPEPEREHALYELGQDFLRAGLLDRAEESLRRLMSGPYAASAKRVLLELYEVEKEWQKAIEAARELQALDQQDYRVQIAQFCCELAQDALLKKRPEDAVEWLRRATQENPANVRAPILLGDVSAAGGDTEGALKQWLAIEAQDAAYVPLVADKVVKAYAALGEQGKALEWLHGLLKGNLAPELLDTAYRTELEVNGPVAAATLMREQLRRQPTLLALTKYFEAQAAENQAAQKQSAESAEPAEGGGDAVIDPQAQETAAIRDLLQLRTRNLARYTCRECGFRARLFYWQCPGCNRWETYAPRRSEALG</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="62" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LQH0</accession>
    <name>Q1LQH0_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF2061 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_0720</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07606.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515569.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0720"/>
    <dbReference type="EnsemblBacteria" id="ABF07606">
        <property type="protein sequence ID" value="ABF07606"/>
        <property type="gene ID" value="Rmet_0720"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0720"/>
    <dbReference type="eggNOG" id="COG3205">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_160691_2_0_4"/>
    <dbReference type="OMA" id="FHEKVWK"/>
    <dbReference type="OrthoDB" id="1574819at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018638">
        <property type="entry name" value="DUF2061_membrane"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09834">
        <property type="entry name" value="DUF2061"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF2061" evidence="1">
        <location>
            <begin position="1"/>
            <end position="52"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF09834"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF07606.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="76" mass="8254" checksum="C7053D00CF134418" modified="2006-05-30" version="1">MAKTLTFGIMHLGIAFSVTYALTGSLAISGAITFIEPAVNTVAHYFFDKFWERRERQAKTLAAPPEATTAARYAAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="114" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LQH1</accession>
    <name>Q1LQH1_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">3-phosphoshikimate 1-carboxyvinyltransferase</fullName>
            <ecNumber evidence="7">2.5.1.19</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">5-enolpyruvylshikimate-3-phosphate synthase</fullName>
            <shortName evidence="7">EPSP synthase</shortName>
            <shortName evidence="7">EPSPS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7 9" type="primary">aroA</name>
        <name evidence="9" type="ordered locus">Rmet_0719</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Catalyzes the transfer of the enolpyruvyl moiety of phosphoenolpyruvate (PEP) to the 5-hydroxyl of shikimate-3-phosphate (S3P) to produce enolpyruvyl shikimate-3-phosphate and inorganic phosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 7">
            <text>3-phosphoshikimate + phosphoenolpyruvate = 5-O-(1-carboxyvinyl)-3-phosphoshikimate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:21256"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57701"/>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
            <dbReference type="ChEBI" id="CHEBI:145989"/>
            <dbReference type="EC" id="2.5.1.19"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 7">Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 6/7.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 7">Belongs to the EPSP synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.19" evidence="7"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07605.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0719"/>
    <dbReference type="EnsemblBacteria" id="ABF07605">
        <property type="protein sequence ID" value="ABF07605"/>
        <property type="gene ID" value="Rmet_0719"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0719"/>
    <dbReference type="eggNOG" id="COG0128">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024321_0_0_4"/>
    <dbReference type="OMA" id="YEDHRMA"/>
    <dbReference type="UniPathway" id="UPA00053">
        <property type="reaction ID" value="UER00089"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003866">
        <property type="term" value="F:3-phosphoshikimate 1-carboxyvinyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009073">
        <property type="term" value="P:aromatic amino acid family biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008652">
        <property type="term" value="P:cellular amino acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009423">
        <property type="term" value="P:chorismate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd01556">
        <property type="entry name" value="EPSP_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.65.10.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00210">
        <property type="entry name" value="EPSP_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001986">
        <property type="entry name" value="Enolpyruvate_Tfrase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036968">
        <property type="entry name" value="Enolpyruvate_Tfrase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006264">
        <property type="entry name" value="EPSP_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023193">
        <property type="entry name" value="EPSP_synthase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013792">
        <property type="entry name" value="RNA3'P_cycl/enolpyr_Trfase_a/b"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00275">
        <property type="entry name" value="EPSP_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000505">
        <property type="entry name" value="EPSPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55205">
        <property type="entry name" value="SSF55205"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01356">
        <property type="entry name" value="aroA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00104">
        <property type="entry name" value="EPSP_SYNTHASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00885">
        <property type="entry name" value="EPSP_SYNTHASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="6 7" id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7 9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="EPSP_synthase" evidence="8">
        <location>
            <begin position="25"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="region of interest" description="Shikimate-3-phosphate binding" evidence="7">
        <location>
            <begin position="37"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="region of interest" description="Phosphoenolpyruvate" evidence="7">
        <location>
            <begin position="106"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="region of interest" description="Shikimate-3-phosphate binding" evidence="7">
        <location>
            <begin position="183"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="7">
        <location>
            <position position="335"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="7">
        <location>
            <position position="363"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="42"/>
        </location>
        <ligand>
            <name>3-phosphoshikimate</name>
            <dbReference type="ChEBI" id="CHEBI:145989"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="136"/>
        </location>
        <ligand>
            <name>phosphoenolpyruvate</name>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="214"/>
        </location>
        <ligand>
            <name>3-phosphoshikimate</name>
            <dbReference type="ChEBI" id="CHEBI:145989"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="362"/>
        </location>
        <ligand>
            <name>3-phosphoshikimate</name>
            <dbReference type="ChEBI" id="CHEBI:145989"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="366"/>
        </location>
        <ligand>
            <name>phosphoenolpyruvate</name>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="413"/>
        </location>
        <ligand>
            <name>phosphoenolpyruvate</name>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="438"/>
        </location>
        <ligand>
            <name>phosphoenolpyruvate</name>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001901"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009948"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ABF07605.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="452" mass="48241" checksum="41C9724B2AFC0ECD" modified="2006-05-30" version="1">MPPARAARNGHAAENMEQLTLGPLTRASGTVRLPGSKSISNRVLLLSALATGETRVRDLLDSDDTRVMLEALKVLGVAWRREGNDCIVSGVGGNFPSKAADLFMGNAGTAIRPLTAALALQGGTYKLSGVQRMHERPIGDLVDGLRQVGASVDYLGTPGYPPLQINPAQIRIDAPIRVRGDVSSQFLTALLMTLPMAQAQSGRIEIEVVGELISKPYIEITLNLLSRFGIAVERQGWERFILPAGARYRSPGEIYVEGDASTASYFLAAGAIGGGPVRVEGVGMASIQGDVRFADALNRMGANVMAGDNWIEVRGTERDDGRLNGIELDCNHIPDAAMTLAVAALFAEGTTTLTNIASWRVKETDRISAMATELRKLGAEVEEGADYLKVTPPTSPATWQTPDAGIGTYDDHRMAMCFSLAAFGPLPVKINDPGCVAKTFPEYFEVFAGVAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="104" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LQH3</accession>
    <name>Q1LQH3_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Histidinol-phosphate aminotransferase</fullName>
            <ecNumber evidence="6">2.6.1.9</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Imidazole acetol-phosphate transaminase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">hisC</name>
        <name evidence="8" type="synonym">hisC1</name>
        <name evidence="8" type="ordered locus">Rmet_0717</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 6">
            <text>2-oxoglutarate + L-histidinol phosphate = 3-(imidazol-4-yl)-2-oxopropyl phosphate + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:23744"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:57766"/>
            <dbReference type="ChEBI" id="CHEBI:57980"/>
            <dbReference type="EC" id="2.6.1.9"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 6">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3 6">Amino-acid biosynthesis; L-histidine biosynthesis; L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 7/9.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4 6">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the class-II pyridoxal-phosphate-dependent aminotransferase family. Histidinol-phosphate aminotransferase subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.6.1.9" evidence="6"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07603.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515566.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0717"/>
    <dbReference type="EnsemblBacteria" id="ABF07603">
        <property type="protein sequence ID" value="ABF07603"/>
        <property type="gene ID" value="Rmet_0717"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0717"/>
    <dbReference type="eggNOG" id="COG0079">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017584_3_3_4"/>
    <dbReference type="OMA" id="YPDMACT"/>
    <dbReference type="OrthoDB" id="1248286at2"/>
    <dbReference type="UniPathway" id="UPA00031">
        <property type="reaction ID" value="UER00012"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004400">
        <property type="term" value="F:histidinol-phosphate transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000105">
        <property type="term" value="P:histidine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01023">
        <property type="entry name" value="HisC_aminotrans_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001917">
        <property type="entry name" value="Aminotrans_II_pyridoxalP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005861">
        <property type="entry name" value="HisP_aminotrans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="SSF53383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01141">
        <property type="entry name" value="hisC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00599">
        <property type="entry name" value="AA_TRANSFER_CLASS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="5 6 8" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="6" id="KW-0368">Histidine biosynthesis</keyword>
    <keyword evidence="6" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aminotran_1_2" evidence="7">
        <location>
            <begin position="42"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="6">
        <location>
            <position position="233"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001045"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ABF07603.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="374" mass="40258" checksum="5E4CA79FA574CD0C" modified="2006-05-30" version="1">MAGQDNAADYFGPSYVRKISPYIAGKPISEVAREFGLDEARIVKLASNENPLGMPASAKAAIAAATEDLGRYPDSNGFELKAKLSEKYDVPAEWLTLGNGSNDILELAAHALVRNGESIVYAEYSFAVYALATQEVGARAIEVKSRAYGHDLDAMAAAIAPDTKLVFIANPNNPTGTFVSAAEIEAFLAKVPPHVVVVLDEAYNEYLDAGQQYESVAWVRKYPNLMVSRTFSKAYGLAGLRVGYGVAQPQLTDLLNRVRQPFNVNSLAQAAAVAALDDTAFLQRSAELNRQGKARLVEAFERSGLEYVKSSGNFVMVRVGGDDGAGARVNLALLKQGVIVRPVANYGLPQWLRVTIGLPEENDAFIAALEQALK</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="63" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LQN8</accession>
    <name>Q1LQN8_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Flp/Fap pilin component Putative pilus subunit protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">pilA</name>
        <name evidence="2" type="ordered locus">Rmet_0652</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07538.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515509.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0652"/>
    <dbReference type="EnsemblBacteria" id="ABF07538">
        <property type="protein sequence ID" value="ABF07538"/>
        <property type="gene ID" value="Rmet_0652"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0652"/>
    <dbReference type="eggNOG" id="COG3847">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_171854_6_1_4"/>
    <dbReference type="OMA" id="AINAKFT"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007047">
        <property type="entry name" value="Flp_Fap"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04964">
        <property type="entry name" value="Flp_Fap"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="41"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF07538.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="57" mass="6010" checksum="5C79DF22DE74528E" modified="2006-05-30" version="1">MSNHFVSLKAFLRDDDGVTAIEYGLIAALIAVVIIASVQLVGTNLSSIFNTIASELS</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LQP9</accession>
    <name>Q1LQP9_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Flp pilus assembly ATPase CpaF</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">cpaF1</name>
        <name evidence="3" type="ordered locus">Rmet_0641</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the GSP E family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07527.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515498.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0641"/>
    <dbReference type="EnsemblBacteria" id="ABF07527">
        <property type="protein sequence ID" value="ABF07527"/>
        <property type="gene ID" value="Rmet_0641"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0641"/>
    <dbReference type="eggNOG" id="COG4962">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005379_4_1_4"/>
    <dbReference type="OMA" id="HDERVIT"/>
    <dbReference type="OrthoDB" id="473670at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001482">
        <property type="entry name" value="T2SS/T4SS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00437">
        <property type="entry name" value="T2SSE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="T2SP_E" evidence="2">
        <location>
            <begin position="103"/>
            <end position="378"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006611"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF07527.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="456" mass="50611" checksum="31E1D36CD5E027C7" modified="2006-05-30" version="1">MSIREQLASAGTPHTANGHAAIHFSMATNTTSPGYHALKRDVHETVLDRVELERLAHFPQEQVRQEIASLVSLIIEEHKVLLNDNERRQLTVEIYDEMFGFGPLEPLLKDPTVSDILVNTARQTYVERRGKLELTNVVFYDDAHLMKVIEKIVSRVGRRIDETSPMVDARLPDGSRVNAIIPPSAIDGPLLSIRRFSVNPLQVSDLVELKSLTQPMAQMLQALAHAKINVLVSGGTGSGKTTLLNILSGFIPEDERVVTIEDAAELQLRQPHVLRLETRPPNIEGKGEITQRALVRNALRMRPDRIILGEIRGGEALDMLNAMNTGHEGSLTTIHANTPRDALTRLENMVSMAGLTMPAKAMRQQIASAITVIVQAARLTDGRRKIISIQEITGMEGDIINTQEIFTFQRTGVAEDGTVRGHFKATGVYPKFAERLRVFGVGLPDETYDPARRYEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="72" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LRI8</accession>
    <name>Q1LRI8_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Porin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">oprB</name>
        <name evidence="3" type="ordered locus">Rmet_0352</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell outer membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the OprB family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07238.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0352"/>
    <dbReference type="EnsemblBacteria" id="ABF07238">
        <property type="protein sequence ID" value="ABF07238"/>
        <property type="gene ID" value="Rmet_0352"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0352"/>
    <dbReference type="eggNOG" id="COG3659">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_545978_0_0_4"/>
    <dbReference type="OMA" id="DPRTQFM"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009279">
        <property type="term" value="C:cell outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046930">
        <property type="term" value="C:pore complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015288">
        <property type="term" value="F:porin activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008643">
        <property type="term" value="P:carbohydrate transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006811">
        <property type="term" value="P:ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.160.180">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007049">
        <property type="entry name" value="Carb-sel_porin_OprB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038673">
        <property type="entry name" value="OprB_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04966">
        <property type="entry name" value="OprB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0998">Cell outer membrane</keyword>
    <keyword evidence="2" id="KW-0406">Ion transport</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0626">Porin</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU363072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF07238.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="492" mass="54215" checksum="579314D2BDC03509" modified="2006-05-30" version="1">MGLWLSCPPIHWMPACAHPLPPIPMSRSCPPAAPQRLRPSFARRFAVPFAALLVAGGLALPWAAQAQEATGPGQEAVSDVESPFAIHGQTTYIWQRKPSFGAGYSGENSLTPDRAKSYSFTATLDLGLRLWQGAEFHFNPEVAQGVPFSELHGVGGPSNGELAKTASTNPKFYRARAFIRQTWGLGGGEEKLEADFNQFARTVDKQRVVLTAGNFSVLDVFDQNPYGSDPRTQFMNWSFLTHGSFDYAADARGYNWGASVEYIGDGWSARFGRFLQPLESNGQELDTRMFEHYGDVLELEKRYQIGGRPGQARLLLWRNKAKMGAFNDAIAFGLANNVTPDVADVRREHAKVGVGLAFLQEVNDSLGLFLRANWSDDKTETYAFTEIGRQVAWGGTLKGTAWGRPKDEVGLGMAINMLGSNHRNYLAAGGLGAFLGDGALNYGPEQVFEIYYSFAVWKTLSLSPDFQYMRNPGYNRDRGPAKFIGIRAHAEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="70" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LRJ8</accession>
    <name>Q1LRJ8_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Glutamate and aspartate transporter subunit periplasmic-binding component of ABC superfamily</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">gltI</name>
        <name evidence="4" type="ordered locus">Rmet_0342</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07228.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515222.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0342"/>
    <dbReference type="EnsemblBacteria" id="ABF07228">
        <property type="protein sequence ID" value="ABF07228"/>
        <property type="gene ID" value="Rmet_0342"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0342"/>
    <dbReference type="eggNOG" id="COG0834">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019602_0_0_4"/>
    <dbReference type="OMA" id="RVAAFMM"/>
    <dbReference type="OrthoDB" id="685252at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001638">
        <property type="entry name" value="Solute-binding_3/MltF_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00497">
        <property type="entry name" value="SBP_bac_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00062">
        <property type="entry name" value="PBPb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193663" evidence="2">
        <location>
            <begin position="25"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="domain" description="PBPb" evidence="3">
        <location>
            <begin position="41"/>
            <end position="273"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00062"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF07228.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="302" mass="32824" checksum="6B5AAF62A48A7481" modified="2006-05-30" version="1">MNQFFMTRAAVLMAVSLGAGSALAVDVAASPTLSKIKSSGTISIGHRTSSIPFSYYDANQKVIGFSQDICDRVIDAVKKETGTPNLQVRMVPVTSQNRQSLVQNGTVDLECGVTTNLKSRQQQVAFSTTFFVAGTRLLVKKGSPVHDFGDLSGKAVVTNAGTTSERILRKLNDEKGANITIQSAKDYGESFLILQSGRVAAFMMDDVLLSGSRTLAPNPNDWVVVGTPQSFEAYGFMMRKDDPGFKQVVDATTTGLMRSGEINTLYTKWFLKPVPPKNLSFDLPQSEQIKKAYANPNDEAFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LRJ9</accession>
    <name>Q1LRJ9_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Major facilitator superfamily (MFS_1) transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Rmet_0341</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07227.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515221.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0341"/>
    <dbReference type="EnsemblBacteria" id="ABF07227">
        <property type="protein sequence ID" value="ABF07227"/>
        <property type="gene ID" value="Rmet_0341"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0341"/>
    <dbReference type="eggNOG" id="COG2271">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001265_0_0_4"/>
    <dbReference type="OMA" id="KGQSICA"/>
    <dbReference type="OrthoDB" id="1345431at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="SSF103473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="36"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="74"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="106"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="131"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="163"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="199"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="269"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="302"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="335"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="361"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="394"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="426"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="domain" description="MFS" evidence="6">
        <location>
            <begin position="40"/>
            <end position="450"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABF07227.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="453" mass="48919" checksum="E010709068C0E15D" modified="2006-05-30" version="1">MHTQSLGAAPVLPEIPSHAIPQVLPTQQEDALYRKVWLRIIPFLFICYVVSFLDRINIGFAQLQMKHDLGFSDAMYGLGAAVFYVGYVLCEVPSNMLLARFGARRTFTRIMLLWGVASVGMMLVSQPSHFYLLRFLLGVFEAGFFPGIVLYLTYWFPARRRAAVMAIFFAGVAVAGVLGGLISGWIMRDMAGVLGMRGWQWMFAIEGAPAVLLGLIAAVCLVDGPRQARWLTPAEQDYLVAQHEAESRGAGGHAHSMQALRQALANPRVYLFAFIYFALTCGSLTLSFWMPLMIRDFGIQDVVAVSLYSVIPNAIGAVGLILIARRSDRLGERHGHFLCCTAGGALALAALTLHVPSLGVSLAILSVAAVLIFAALPIFWALPPSHLPAQTAATGIAFISSIGITSGIVSPWVIGQIKAHTGSMDNALYLLAGLLLASGLALWKGVPRTPTAH</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LRS7</accession>
    <name>Q1LRS7_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Cytochrome c oxidase assembly protein CtaG</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">ctaG</name>
        <name evidence="11" type="ordered locus">Rmet_0263</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Exerts its effect at some terminal stage of cytochrome c oxidase synthesis, probably by being involved in the insertion of the copper B into subunit I.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell inner membrane</location>
            <topology evidence="2">Single-pass type II membrane protein</topology>
            <orientation evidence="2">Periplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the COX11/CtaG family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07149.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515155.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0263"/>
    <dbReference type="EnsemblBacteria" id="ABF07149">
        <property type="protein sequence ID" value="ABF07149"/>
        <property type="gene ID" value="Rmet_0263"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0263"/>
    <dbReference type="eggNOG" id="COG3175">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045000_5_2_4"/>
    <dbReference type="OMA" id="NKLECFC"/>
    <dbReference type="OrthoDB" id="1821272at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.370.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023471">
        <property type="entry name" value="CtaG/Cox11_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007533">
        <property type="entry name" value="Cyt_c_oxidase_assmbl_CtaG"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04442">
        <property type="entry name" value="CtaG_Cox11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005413">
        <property type="entry name" value="COX11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110111">
        <property type="entry name" value="SSF110111"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0186">Copper</keyword>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="5 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="20"/>
            <end position="38"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009620"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00015384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ABF07149.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="198" mass="22057" checksum="175FF88D7D78A346" modified="2006-05-30" version="1">MDGQDKAIEKRFNRGMMLRLLVVVTLMFGFGYALVPLYKAICEVTGINVLTTRDLYGGSALKNSQVDTSRTITVEFDSNARGPFAFRPVKHSMEVHPGELTTIVYEVANGQSRDISAQAIPSYAPKQATEYFKKIECFCFKQQTLKSKEAREMPVVFVIDPKLPKDVKTITLSYTFFEVGTPVAQAPQDEQASVQKGS</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-09-02" modified="2022-08-03" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LS06</accession>
    <name>TTCA_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">tRNA-cytidine(32) 2-sulfurtransferase</fullName>
            <ecNumber evidence="1">2.8.1.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Two-thiocytidine biosynthesis protein A</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">tRNA 2-thiocytidine biosynthesis protein TtcA</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">ttcA</name>
        <name type="ordered locus">Rmet_0184</name>
    </gene>
    <organism>
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the ATP-dependent 2-thiolation of cytidine in position 32 of tRNA, to form 2-thiocytidine (s(2)C32). The sulfur atoms are provided by the cysteine/cysteine desulfurase (IscS) system.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>AH2 + ATP + cytidine(32) in tRNA + S-sulfanyl-L-cysteinyl-[cysteine desulfurase] = 2-thiocytidine(32) in tRNA + A + AMP + diphosphate + H(+) + L-cysteinyl-[cysteine desulfurase]</text>
            <dbReference type="Rhea" id="RHEA:57048"/>
            <dbReference type="Rhea" id="RHEA-COMP:10288"/>
            <dbReference type="Rhea" id="RHEA-COMP:12157"/>
            <dbReference type="Rhea" id="RHEA-COMP:12158"/>
            <dbReference type="Rhea" id="RHEA-COMP:14821"/>
            <dbReference type="ChEBI" id="CHEBI:13193"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17499"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61963"/>
            <dbReference type="ChEBI" id="CHEBI:82748"/>
            <dbReference type="ChEBI" id="CHEBI:141453"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="1">
            <dbReference type="Rhea" id="RHEA:57049"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="1">Binds 1 [4Fe-4S] cluster per subunit. The cluster is chelated by three Cys residues, the fourth Fe has a free coordination site that may bind a sulfur atom transferred from the persulfide of IscS.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">tRNA modification.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="miscellaneous">
        <text evidence="1">The thiolation reaction likely consists of two steps: a first activation step by ATP to form an adenylated intermediate of the target base of tRNA, and a second nucleophilic substitution step of the sulfur (S) atom supplied by the hydrosulfide attached to the Fe-S cluster.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the TtcA family.</text>
    </comment>
    <dbReference type="EC" id="2.8.1.-" evidence="1"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07070.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515091.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q1LS06"/>
    <dbReference type="SMR" id="Q1LS06"/>
    <dbReference type="STRING" id="266264.Rmet_0184"/>
    <dbReference type="EnsemblBacteria" id="ABF07070">
        <property type="protein sequence ID" value="ABF07070"/>
        <property type="gene ID" value="Rmet_0184"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0184"/>
    <dbReference type="eggNOG" id="COG0037">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026481_0_0_4"/>
    <dbReference type="OMA" id="MNLDQKQ"/>
    <dbReference type="OrthoDB" id="1051352at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016783">
        <property type="term" value="F:sulfurtransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034227">
        <property type="term" value="P:tRNA thio-modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01850">
        <property type="entry name" value="TtcA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011063">
        <property type="entry name" value="TilS/TtcA_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012089">
        <property type="entry name" value="tRNA_Cyd_32_2_STrfase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01171">
        <property type="entry name" value="ATP_bind_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0004">4Fe-4S</keyword>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0408">Iron</keyword>
    <keyword id="KW-0411">Iron-sulfur</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <keyword id="KW-0819">tRNA processing</keyword>
    <keyword id="KW-0820">tRNA-binding</keyword>
    <feature type="chain" id="PRO_0000348812" description="tRNA-cytidine(32) 2-sulfurtransferase">
        <location>
            <begin position="1"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="PP-loop motif" evidence="1">
        <location>
            <begin position="39"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="114"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="117"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="205"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01850"/>
        </source>
    </evidence>
    <sequence length="314" mass="34865" checksum="3769E8C49B8E461C" modified="2006-05-30" version="1">MSHSANFYRLETRLQSLTGKAIGDFGMIEDGDTVLVCMSGGKDSYTMLSVLTALQKRAPIQFKLIAMNLDQKQPGFPEHVLPAYLKSLGVEYVIVEADTYSIVKEKVPEGKTTCSLCSRLRRGVIYRTAKELGANKIALGHHRDDIVNTFFLNMFFGGKMKAMPPKLATDDGQHIVIRPLAYCAEKDIASYARAMAFPIIPCNLCGSQENLQRKKVSEMLQAWERENPGRIDNIFAALRNVVPSHLADTDLFPFTGLATGLAKIDEASLFGETTFTQQALTFTGNVEANRMEFVRFDKIQKVQEAPERASEPSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="68" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LSC1</accession>
    <name>Q1LSC1_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative lipoprotein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_0069</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF06955.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011514994.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0069"/>
    <dbReference type="EnsemblBacteria" id="ABF06955">
        <property type="protein sequence ID" value="ABF06955"/>
        <property type="gene ID" value="Rmet_0069"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0069"/>
    <dbReference type="eggNOG" id="COG3218">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_091341_1_0_4"/>
    <dbReference type="OMA" id="YANSHWT"/>
    <dbReference type="OrthoDB" id="1526230at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005586">
        <property type="entry name" value="ABC_trans_aux"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03886">
        <property type="entry name" value="ABC_trans_aux"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193486" evidence="1">
        <location>
            <begin position="26"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="domain" description="ABC_trans_aux" evidence="2">
        <location>
            <begin position="39"/>
            <end position="188"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF03886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF06955.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="215" mass="22737" checksum="DEAB30211B746920" modified="2006-05-30" version="1">MTIASALRFTALGLLIALSGCSVLSSQPPSTTYDFGPLAAAPSQPPAPLPKLRITQTDGPVWMDGSGIYYRLQYTQAQRLQAYSTQRWVDSPQHLFNDRLRDAVAARGPLSWSGDTTIPALKVDLLAFEQVFDSPTSSRGVVRARATVYHKGLIGQKTFVAEQPAPSADGQGGVKALSAGVDAVIAAILDWTATLPLDGAQASSAPPAPPAPQRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LSC8</accession>
    <name>Q1LSC8_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Putative allophanate hydrolase subunit 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Rmet_0062</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF06948.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011514987.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0062"/>
    <dbReference type="EnsemblBacteria" id="ABF06948">
        <property type="protein sequence ID" value="ABF06948"/>
        <property type="gene ID" value="Rmet_0062"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0062"/>
    <dbReference type="eggNOG" id="COG2049">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020207_0_0_4"/>
    <dbReference type="OMA" id="QPGFAYM"/>
    <dbReference type="OrthoDB" id="1848526at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.100.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003833">
        <property type="entry name" value="CT_C_D"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029000">
        <property type="entry name" value="Cyclophilin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010016">
        <property type="entry name" value="KipI_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34698">
        <property type="entry name" value="PTHR34698"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02682">
        <property type="entry name" value="CT_C_D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00796">
        <property type="entry name" value="AHS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50891">
        <property type="entry name" value="SSF50891"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00370">
        <property type="entry name" value="TIGR00370"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AHS1" evidence="4">
        <location>
            <begin position="4"/>
            <end position="201"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00796"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABF06948.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="216" mass="23127" checksum="56E75CC98CE9E972" modified="2006-05-30" version="1">MPQCTVHRLAEQALLYSVEPPASLDVQRRIWAMAARAADWRGVVDVVPGMNNLTVTFDGTADVAALERNLKLAWASGEARNATGRLVEVPVRYGGEFGPDLADVAAHTGLTPAEVVHRHAAGEYVVYFLGFQPGFTYMGGLAPELATPRRREPRLAVPAGAVGIGGEQTGIYPAVLPGGWQLIGRTDAQLFVADRDPPSLFAPGDTVRFVAEEILL</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-04-26" modified="2021-09-29" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>Q58AC6</accession>
    <name>Q58AC6_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative hemagglutinin-related transmembrane</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_6093</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2 3">
        <name>pMOL30</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid pMOL30</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000354">
        <property type="protein sequence ID" value="ABF12952.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011229408.1">
        <property type="nucleotide sequence ID" value="NC_006466.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_145692.1">
        <property type="nucleotide sequence ID" value="NC_006466.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF12952">
        <property type="protein sequence ID" value="ABF12952"/>
        <property type="gene ID" value="Rmet_6093"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6093"/>
    <dbReference type="HOGENOM" id="CLU_034937_0_0_4"/>
    <dbReference type="OMA" id="TAMMMED"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid pMOL30"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019501">
        <property type="entry name" value="Peptidase_M30_hyicolysin"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10460">
        <property type="entry name" value="Peptidase_M30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="57"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="63"/>
            <end position="90"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF12952.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="595" mass="63475" checksum="CDE1FD0856CD0C9B" modified="2005-04-26" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2005-04-26" modified="2022-08-03" version="112" xmlns="http://uniprot.org/uniprot">
    <accession>Q58AR3</accession>
    <name>Q58AR3_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Ribonuclease VapC</fullName>
            <shortName evidence="5">RNase VapC</shortName>
            <ecNumber evidence="5">3.1.-.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Toxin VapC</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5" type="primary">vapC</name>
        <name evidence="7" type="ordered locus">Rmet_6032</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="7 8">
        <name>pMOL30</name>
    </geneLocation>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="8">Plasmid pMOL30</plasmid>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Toxic component of a toxin-antitoxin (TA) system. An RNase.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the PINc/VapC protein family.</text>
    </comment>
    <dbReference type="EC" id="3.1.-.-" evidence="5"/>
    <dbReference type="EMBL" id="CP000354">
        <property type="protein sequence ID" value="ABF12891.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011229306.1">
        <property type="nucleotide sequence ID" value="NC_007971.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_145552.1">
        <property type="nucleotide sequence ID" value="NC_006466.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF12891">
        <property type="protein sequence ID" value="ABF12891"/>
        <property type="gene ID" value="Rmet_6032"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6032"/>
    <dbReference type="HOGENOM" id="CLU_147393_0_0_4"/>
    <dbReference type="OMA" id="IDHFRHA"/>
    <dbReference type="OrthoDB" id="1968541at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid pMOL30"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004540">
        <property type="term" value="F:ribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090729">
        <property type="term" value="F:toxin activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00265">
        <property type="entry name" value="VapC_Nob1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029060">
        <property type="entry name" value="PIN-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002716">
        <property type="entry name" value="PIN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022907">
        <property type="entry name" value="VapC_family"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01850">
        <property type="entry name" value="PIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88723">
        <property type="entry name" value="SSF88723"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3 5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2 5" id="KW-0540">Nuclease</keyword>
    <keyword evidence="7" id="KW-0614">Plasmid</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0800">Toxin</keyword>
    <keyword evidence="1 5" id="KW-1277">Toxin-antitoxin system</keyword>
    <feature type="domain" description="PINc" evidence="6">
        <location>
            <begin position="2"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="5"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="86"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00265"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABF12891.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="123" mass="13614" checksum="6ED4C6D18047402F" modified="2005-04-26" version="1">MILVDTSVWIDHLNASDPMLISLLTQERVLIHPYVIGEISLGSLRDRDVVLGALRDLPRAPVATPEETLYLIEREGLFNRGIGYGDTSLLASARLQPGIAIWTRDKRLKKVADELCLGAVLSH</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-03-23" modified="2020-10-07" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>D3DXS2</accession>
    <name>D3DXS2_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_6484</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ADC45092.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADC45092">
        <property type="protein sequence ID" value="ADC45092"/>
        <property type="gene ID" value="Rmet_6484"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6484"/>
    <dbReference type="HOGENOM" id="CLU_2719417_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="33"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADC45092.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="72" mass="7879" checksum="685C4937991DA0C6" modified="2010-03-23" version="1">MIPVFLALASAAFSAGCSSFDFWRGTYEGARSHDAAVNPMTPGTVRQTLPPYEDYERERNRARGGNPSIQGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-03-23" modified="2020-10-07" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>D3DY02</accession>
    <name>D3DY02_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_6569</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ADC45172.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADC45172">
        <property type="protein sequence ID" value="ADC45172"/>
        <property type="gene ID" value="Rmet_6569"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6569"/>
    <dbReference type="HOGENOM" id="CLU_2587193_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="22"/>
            <end position="47"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADC45172.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="80" mass="8750" checksum="708777C6F35EED44" modified="2010-03-23" version="1">MSCELNVMVRSNLPSAVDGLRGRPGLGEYSAEPARRNRNPENETAGVGCRPHSFDFVDFRSARCFIHFADLPPLGSLTAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-03-23" modified="2020-10-07" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>D3DY95</accession>
    <name>D3DY95_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_6677</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ADC45265.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADC45265">
        <property type="protein sequence ID" value="ADC45265"/>
        <property type="gene ID" value="Rmet_6677"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6677"/>
    <dbReference type="HOGENOM" id="CLU_3029136_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADC45265.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="55" mass="6186" checksum="C4F4F5ADADE61E47" modified="2010-03-23" version="1">MNIGIEAETPNSIIGHFFDEARREGVSDCNLLHCSPQHMPDQYNLSFVQGSDRHA</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-03-23" modified="2020-10-07" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>D3DYD9</accession>
    <name>D3DYD9_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_6730</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ADC45309.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_6730"/>
    <dbReference type="EnsemblBacteria" id="ADC45309">
        <property type="protein sequence ID" value="ADC45309"/>
        <property type="gene ID" value="Rmet_6730"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6730"/>
    <dbReference type="HOGENOM" id="CLU_2207900_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADC45309.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="107" mass="11414" checksum="B24D60F31E2359B9" modified="2010-03-23" version="1">MESPQLGRPNPFSGRAAQRAIRWFWKHSLGEIRALYGYTVYPISSRKKASCGVPAGWDSYPGAIAIWAIVGCSMGNVGPTHEIAPCPASWSACSTRDQEGVAAFAGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-03-23" modified="2020-10-07" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>D3DYF6</accession>
    <name>D3DYF6_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_6747</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ADC45326.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_6747"/>
    <dbReference type="EnsemblBacteria" id="ADC45326">
        <property type="protein sequence ID" value="ADC45326"/>
        <property type="gene ID" value="Rmet_6747"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6747"/>
    <dbReference type="HOGENOM" id="CLU_2790989_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADC45326.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="68" mass="7432" checksum="4767763ACFA363B9" modified="2010-03-23" version="1">MPHSPWRNTQCPSDGCAMATNQLNAGQSRFRPNSNLIGKVADTSKKALDALIPRVTMTSYHICEVGDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-03-23" modified="2020-10-07" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>D3DYH7</accession>
    <name>D3DYH7_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_6770</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ADC45347.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_6770"/>
    <dbReference type="EnsemblBacteria" id="ADC45347">
        <property type="protein sequence ID" value="ADC45347"/>
        <property type="gene ID" value="Rmet_6770"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6770"/>
    <dbReference type="HOGENOM" id="CLU_2331656_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADC45347.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="98" mass="11002" checksum="873EE4815EBE4D49" modified="2010-03-23" version="1">MRGRRLWRGCLRQPIPQRAAQLLTGRFLTRAGDWSLLFVARGSTPVYVLRPPAGTFFSEGGSHPCTLESLPPSRWPCSRALRRRPWQATTHTSTGPTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-10-07" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>Q1L9J4</accession>
    <name>Q1L9J4_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_6323</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="1 2">
        <name>pMOL28</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid pMOL28</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000355">
        <property type="protein sequence ID" value="ABF13182.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF13182">
        <property type="protein sequence ID" value="ABF13182"/>
        <property type="gene ID" value="Rmet_6323"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6323"/>
    <dbReference type="HOGENOM" id="CLU_1465591_0_0_4"/>
    <dbReference type="OMA" id="GGFYGFE"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid pMOL28"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF13182.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="209" mass="24085" checksum="D375E7F9D8966FAF" modified="2006-05-30" version="1">MNWWLDIQSREIQWAHAYRRLTMYHYITPEVLEDLFAGQPPAGLQPDVIDITEPLSLDEGIEVIGILFAGLRFVVFFADVLVYALGDPEWIEETADEWANGFQPAPAARLVKFMRADADADTMFDPEAWPLPHPRLIWQFCEVLARALSIHAQFFPGIPQYFYMTQSSQLDLLYNRLGRRFEAGAYGPRFECVTRPNADTGGFYGFERR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LAN9</accession>
    <name>Q1LAN9_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Putative membrane carboxypeptidase (Penicillin-binding) transmembrane protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">Rmet_5928</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="10">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="10">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12787.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5928"/>
    <dbReference type="EnsemblBacteria" id="ABF12787">
        <property type="protein sequence ID" value="ABF12787"/>
        <property type="gene ID" value="Rmet_5928"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5928"/>
    <dbReference type="eggNOG" id="COG0744">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG0768">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012369_0_0_4"/>
    <dbReference type="OMA" id="WLFKSRH"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004180">
        <property type="term" value="F:carboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009252">
        <property type="term" value="P:peptidoglycan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008360">
        <property type="term" value="P:regulation of cell shape"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3810.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.710.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012338">
        <property type="entry name" value="Beta-lactam/transpept-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001264">
        <property type="entry name" value="Glyco_trans_51"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023346">
        <property type="entry name" value="Lysozyme-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036950">
        <property type="entry name" value="PBP_transglycosylase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00912">
        <property type="entry name" value="Transgly"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53955">
        <property type="entry name" value="SSF53955"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56601">
        <property type="entry name" value="SSF56601"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-0121">Carboxypeptidase</keyword>
    <keyword evidence="3" id="KW-0133">Cell shape</keyword>
    <keyword evidence="6" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="1" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0573">Peptidoglycan synthesis</keyword>
    <keyword evidence="9" id="KW-0614">Plasmid</keyword>
    <keyword evidence="9" id="KW-0645">Protease</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <keyword evidence="2 7 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="9"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="domain" description="Transgly" evidence="8">
        <location>
            <begin position="136"/>
            <end position="324"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ABF12787.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="1022" mass="114737" checksum="220D6D41D2D5EA05" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-10-07" version="62" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LAT7</accession>
    <name>Q1LAT7_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_5880</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12739.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5880"/>
    <dbReference type="EnsemblBacteria" id="ABF12739">
        <property type="protein sequence ID" value="ABF12739"/>
        <property type="gene ID" value="Rmet_5880"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5880"/>
    <dbReference type="eggNOG" id="COG3185">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012617_1_1_4"/>
    <dbReference type="OMA" id="KVNVCFE"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010839">
        <property type="entry name" value="AtuA"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07287">
        <property type="entry name" value="AtuA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF12739.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="466" mass="48729" checksum="607F3A37C2DDD5C5" modified="2006-05-30" version="1">MGSPSFSSSFGNAMTVPLLIGSGAGFSGDRTDAALPVVRTLIAAGGPAALIFETLAERTLALAQLARRDDPEHGYEPLLDQLLTPVLGLCLVNDIPIVGNFGAANPRAAARRIGELAVELGLPVPRVAVVEGDDLSHDEGRALLRGIVGDTFPEARFVCANAYIGAQGIADALRDGAQVVVCGRVADPALAVGPAMAHFGWAWDDWNRLAAATMAGHLLECGAQVSGGYFADPGMKDVPDVHAVGFPIARLDADGGIEIFKAAGTGGCVDLRTVKEQLLYEVHDPCAYLTPDVVADIGEVTVAQVGPDRVAVRNIRGHARPDTLKVNLFYQGGWIAEGEISYAGPNAAARARLAADILGKRMKGLGFDVPIRFDLIGVLSVFADDAGEMLARPQPHEAEARDVRLRAALAHEDKAVAQALLREVNALYTCGPAGGGGVRTALRARLNAMSCLAPRSAVAPRHTLLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="102" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LB11</accession>
    <name>Q1LB11_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Ferric citrate outer membrane transporter KpLE2 phage-like element</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">fecA1</name>
        <name evidence="11" type="ordered locus">Rmet_5806</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="12">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="12">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cell outer membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the TonB-dependent receptor family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12665.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5806"/>
    <dbReference type="EnsemblBacteria" id="ABF12665">
        <property type="protein sequence ID" value="ABF12665"/>
        <property type="gene ID" value="Rmet_5806"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5806"/>
    <dbReference type="eggNOG" id="COG4772">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008287_17_1_4"/>
    <dbReference type="OMA" id="QYNNENA"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009279">
        <property type="term" value="C:cell outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015344">
        <property type="term" value="F:siderophore uptake transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038023">
        <property type="term" value="F:signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.130.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.170.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012910">
        <property type="entry name" value="Plug_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037066">
        <property type="entry name" value="Plug_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000531">
        <property type="entry name" value="TonB-dep_rcpt_b-brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036942">
        <property type="entry name" value="TonB_rcpt_b-brl_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010105">
        <property type="entry name" value="TonB_sidphr_rcpt"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07715">
        <property type="entry name" value="Plug"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00593">
        <property type="entry name" value="TonB_dep_Rec"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01783">
        <property type="entry name" value="TonB-siderophor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0998">Cell outer membrane</keyword>
    <keyword evidence="3 6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-0675">Receptor</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0798">TonB box</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="domain" description="Plug" evidence="10">
        <location>
            <begin position="73"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="domain" description="TonB_dep_Rec" evidence="9">
        <location>
            <begin position="272"/>
            <end position="728"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="643"/>
            <end position="662"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009810"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003357"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00593"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF07715"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ABF12665.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="731" mass="79781" checksum="7CF11889D55C9165" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="100" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LB26</accession>
    <name>Q1LB26_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Cytochrome bo(3) ubiquinol oxidase subunit 3</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Cytochrome o ubiquinol oxidase subunit 3</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">Oxidase bo(3) subunit 3</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="15">Ubiquinol oxidase polypeptide III</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">Ubiquinol oxidase subunit 3</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="primary">cyoC</name>
        <name evidence="19" type="ordered locus">Rmet_5791</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="20">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="20" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="20">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Cytochrome bo(3) ubiquinol terminal oxidase is the component of the aerobic respiratory chain of E.coli that predominates when cells are grown at high aeration. Has proton pump activity across the membrane in addition to electron transfer, pumping 2 protons/electron.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Heterooctamer of two A chains, two B chains, two C chains and two D chains.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="16">Cell membrane</location>
            <topology evidence="16">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 16">Belongs to the cytochrome c oxidase subunit 3 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12650.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5791"/>
    <dbReference type="EnsemblBacteria" id="ABF12650">
        <property type="protein sequence ID" value="ABF12650"/>
        <property type="gene ID" value="Rmet_5791"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5791"/>
    <dbReference type="eggNOG" id="COG1845">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_044071_3_0_4"/>
    <dbReference type="OMA" id="MWFFVIG"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009486">
        <property type="term" value="F:cytochrome bo3 ubiquinol oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004129">
        <property type="term" value="F:cytochrome-c oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019646">
        <property type="term" value="P:aerobic electron transport chain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02863">
        <property type="entry name" value="Ubiquinol_oxidase_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024791">
        <property type="entry name" value="Cyt_c/ubiquinol_Oxase_su3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000298">
        <property type="entry name" value="Cyt_c_oxidase-like_su3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035973">
        <property type="entry name" value="Cyt_c_oxidase_su3-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013833">
        <property type="entry name" value="Cyt_c_oxidase_su3_a-hlx"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014206">
        <property type="entry name" value="Cyt_c_ubiqinol_oxidase_su3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033946">
        <property type="entry name" value="Ubiquinol_oxase_su3_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11403">
        <property type="entry name" value="PTHR11403"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00510">
        <property type="entry name" value="COX3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81452">
        <property type="entry name" value="SSF81452"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02842">
        <property type="entry name" value="CyoC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50253">
        <property type="entry name" value="COX3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-0249">Electron transport</keyword>
    <keyword evidence="10 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="19" id="KW-0614">Plasmid</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 16 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 17" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="49"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="88"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="120"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="160"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="204"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="domain" description="COX3" evidence="18">
        <location>
            <begin position="28"/>
            <end position="225"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010581"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011700"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00014687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00025694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00030072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00031884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00032189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00032717"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU003376"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50253"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="ABF12650.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="226" mass="24823" checksum="6B0661DE8DB87B51" modified="2006-05-30" version="1">MTTSLKLRADAAPGIHAPGHASDAHGHDAAHGHDGHDDHVVQSRTTLGFWLYLMSDCLIFAVLFATFGVLVNNTAGGPGGRELFDLRFVLGETMLLLTSSLTFGVAMIKLEDGKANPNQVIRWIVLTFALGAAFVGMEIFEFMELIHEGAGPGRSAYLSAFFMLVGTHGLHVTAGLLWIIVMVHQIQVFGLDGIVRRRLACLSLFWHFLDLVWICVFSIVYLRTFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-10-07" version="50" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LBK2</accession>
    <name>Q1LBK2_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_5615</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12474.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5615"/>
    <dbReference type="EnsemblBacteria" id="ABF12474">
        <property type="protein sequence ID" value="ABF12474"/>
        <property type="gene ID" value="Rmet_5615"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5615"/>
    <dbReference type="HOGENOM" id="CLU_1947049_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="60"/>
            <end position="82"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF12474.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="129" mass="14489" checksum="15AAB5D4296727CD" modified="2006-05-30" version="1">MSWVSARRCGEFLMMALLVRYAVSLARSWDQCFPVATRQCPASGADPSEFPVRYMRGVHATPEESAPTRKARPDAAVWPARGKNRPPTQNYCIEVIPMSWVLMHNFLNLGLKDCIDACDDSNHDDSRSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="56" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LBU1</accession>
    <name>Q1LBU1_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF6285 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_5526</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12385.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519929.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5526"/>
    <dbReference type="EnsemblBacteria" id="ABF12385">
        <property type="protein sequence ID" value="ABF12385"/>
        <property type="gene ID" value="Rmet_5526"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5526"/>
    <dbReference type="eggNOG" id="ENOG5032UR4">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_142892_1_0_4"/>
    <dbReference type="OMA" id="AVDQPNY"/>
    <dbReference type="OrthoDB" id="2054647at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046252">
        <property type="entry name" value="DUF6285"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19802">
        <property type="entry name" value="DUF6285"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF6285" evidence="1">
        <location>
            <begin position="25"/>
            <end position="115"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF19802"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF12385.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="121" mass="13514" checksum="12F66EB76421A30A" modified="2006-05-30" version="1">MSTFVPDANRLLQAAAEYLERELLPTLTGYHGFQTRVCINVLKIVARELEMRPEAQARERRRLEDILGKQGDVGALNRELATRLGAGDLPLDHVGVVEHLISTLQDALSINSPRWAKQVSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="117" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LBV6</accession>
    <name>Q1LBV6_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="12">3-hydroxybutyryl-CoA epimerase (3-hydroxyacyl-CoA dehydrogenase, NAD-binding)</fullName>
            <ecNumber evidence="12">5.1.2.3</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">Rmet_5511</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="13">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="13">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>a (3S)-3-hydroxyacyl-CoA + NAD(+) = a 3-oxoacyl-CoA + H(+) + NADH</text>
            <dbReference type="Rhea" id="RHEA:22432"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57318"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:90726"/>
            <dbReference type="EC" id="1.1.1.35"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Lipid metabolism; fatty acid beta-oxidation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the enoyl-CoA hydratase/isomerase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">In the N-terminal section; belongs to the enoyl-CoA hydratase/isomerase family.</text>
    </comment>
    <dbReference type="EC" id="5.1.2.3" evidence="12"/>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12370.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519913.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5511"/>
    <dbReference type="EnsemblBacteria" id="ABF12370">
        <property type="protein sequence ID" value="ABF12370"/>
        <property type="gene ID" value="Rmet_5511"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5511"/>
    <dbReference type="eggNOG" id="COG1024">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG1250">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009834_15_3_4"/>
    <dbReference type="OMA" id="PFRYMDT"/>
    <dbReference type="OrthoDB" id="977512at2"/>
    <dbReference type="UniPathway" id="UPA00659"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003857">
        <property type="term" value="F:3-hydroxyacyl-CoA dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008692">
        <property type="term" value="F:3-hydroxybutyryl-CoA epimerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070403">
        <property type="term" value="F:NAD+ binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006635">
        <property type="term" value="P:fatty acid beta-oxidation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006176">
        <property type="entry name" value="3-OHacyl-CoA_DH_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006108">
        <property type="entry name" value="3HC_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008927">
        <property type="entry name" value="6-PGluconate_DH-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029045">
        <property type="entry name" value="ClpP/crotonase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018376">
        <property type="entry name" value="Enoyl-CoA_hyd/isom_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001753">
        <property type="entry name" value="Enoyl-CoA_hydra/iso"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00725">
        <property type="entry name" value="3HCDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02737">
        <property type="entry name" value="3HCDH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00378">
        <property type="entry name" value="ECH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48179">
        <property type="entry name" value="SSF48179"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52096">
        <property type="entry name" value="SSF52096"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00166">
        <property type="entry name" value="ENOYL_COA_HYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="12" id="KW-0413">Isomerase</keyword>
    <keyword evidence="5" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="7" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="6" id="KW-0520">NAD</keyword>
    <keyword evidence="12" id="KW-0614">Plasmid</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="3HCDH_N" evidence="11">
        <location>
            <begin position="318"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="domain" description="3HCDH" evidence="10">
        <location>
            <begin position="498"/>
            <end position="598"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023693"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF02737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ABF12370.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="714" mass="76207" checksum="FC20BDD7E8D9D49C" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LBY0</accession>
    <name>Q1LBY0_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DNA helicase</fullName>
            <ecNumber evidence="3">3.6.4.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Rmet_5487</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="9">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="9">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + H2O = ADP + H(+) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.12"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the helicase family. UvrD subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.12" evidence="3"/>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12346.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519888.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5487"/>
    <dbReference type="EnsemblBacteria" id="ABF12346">
        <property type="protein sequence ID" value="ABF12346"/>
        <property type="gene ID" value="Rmet_5487"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5487"/>
    <dbReference type="eggNOG" id="COG0210">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004585_5_10_4"/>
    <dbReference type="OMA" id="ITYSQEC"/>
    <dbReference type="OrthoDB" id="137860at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003678">
        <property type="term" value="F:DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.160">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013986">
        <property type="entry name" value="DExx_box_DNA_helicase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014017">
        <property type="entry name" value="DNA_helicase_UvrD-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000212">
        <property type="entry name" value="DNA_helicase_UvrD/REP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014016">
        <property type="entry name" value="UvrD-like_ATP-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11070">
        <property type="entry name" value="PTHR11070"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13361">
        <property type="entry name" value="UvrD_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51198">
        <property type="entry name" value="UVRD_HELICASE_ATP_BIND"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 8" id="KW-0347">Helicase</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0614">Plasmid</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UvrD-like helicase ATP-binding" evidence="7">
        <location>
            <begin position="13"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="34"/>
            <end position="41"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001665"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ABF12346.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="591" mass="65689" checksum="E88C06A4FE343EAC" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-10-07" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LC51</accession>
    <name>Q1LC51_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Arylsulfatase-like enzyme</fullName>
            <ecNumber evidence="5">3.1.6.1</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">aslA</name>
        <name evidence="5" type="ordered locus">Rmet_5416</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="6">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="6">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="PTM">
        <text evidence="1">The conversion to 3-oxoalanine (also known as C-formylglycine, FGly), of a serine or cysteine residue in prokaryotes and of a cysteine residue in eukaryotes, is critical for catalytic activity.</text>
    </comment>
    <dbReference type="EC" id="3.1.6.1" evidence="5"/>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12275.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5416"/>
    <dbReference type="EnsemblBacteria" id="ABF12275">
        <property type="protein sequence ID" value="ABF12275"/>
        <property type="gene ID" value="Rmet_5416"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5416"/>
    <dbReference type="eggNOG" id="COG3119">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006332_10_0_4"/>
    <dbReference type="OMA" id="IMHHMDW"/>
    <dbReference type="OrthoDB" id="1067869at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004065">
        <property type="term" value="F:arylsulfatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.720.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017850">
        <property type="entry name" value="Alkaline_phosphatase_core_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000917">
        <property type="entry name" value="Sulfatase_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00884">
        <property type="entry name" value="Sulfatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53649">
        <property type="entry name" value="SSF53649"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0614">Plasmid</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193304" evidence="3">
        <location>
            <begin position="28"/>
            <end position="579"/>
        </location>
    </feature>
    <feature type="domain" description="Sulfatase" evidence="4">
        <location>
            <begin position="88"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="33"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="modified residue" description="3-oxoalanine (Cys)" evidence="1">
        <location>
            <position position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRSR" id="PIRSR600917-51"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABF12275.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="579" mass="63723" checksum="0BEC54D6880C8D39" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="67" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LC69</accession>
    <name>Q1LC69_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF305 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Rmet_5398</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="5">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="5">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12257.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519805.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5398"/>
    <dbReference type="EnsemblBacteria" id="ABF12257">
        <property type="protein sequence ID" value="ABF12257"/>
        <property type="gene ID" value="Rmet_5398"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5398"/>
    <dbReference type="eggNOG" id="COG3544">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_074343_4_2_4"/>
    <dbReference type="OMA" id="TRMMDGM"/>
    <dbReference type="OrthoDB" id="1714516at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1260.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005183">
        <property type="entry name" value="DUF305"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012347">
        <property type="entry name" value="Ferritin-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03713">
        <property type="entry name" value="DUF305"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0614">Plasmid</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193309" description="DUF305 domain-containing protein" evidence="2">
        <location>
            <begin position="32"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="domain" description="DUF305" evidence="3">
        <location>
            <begin position="32"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="117"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="120"/>
            <end position="136"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF03713"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF12257.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="152" mass="16025" checksum="22F7AACBC5AC6E11" modified="2006-05-30" version="1">MVSFYRLPTPPAIGAVLLLLAALTAAAPALAATATGSDAERFAAENEVAMTRMMDGMSVHPSGNIDEDFAAMMIPHHQGAIDMAMAELRYGKNEQLRRIAQEIIVDQMQEIAAMRLALGQPPPPSVPAPTQPAASTPSPSQPASHDHSAHAH</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="61" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LCB2</accession>
    <name>Q1LCB2_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_5355</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12214.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519760.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5355"/>
    <dbReference type="EnsemblBacteria" id="ABF12214">
        <property type="protein sequence ID" value="ABF12214"/>
        <property type="gene ID" value="Rmet_5355"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5355"/>
    <dbReference type="eggNOG" id="COG4786">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_078227_0_0_4"/>
    <dbReference type="OMA" id="NDNMIAG"/>
    <dbReference type="OrthoDB" id="1456527at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021556">
        <property type="entry name" value="DUF2950"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11453">
        <property type="entry name" value="DUF2950"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193324" evidence="1">
        <location>
            <begin position="33"/>
            <end position="301"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF12214.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="301" mass="32333" checksum="DD63D4F5EAC581B6" modified="2006-05-30" version="1">MTLTTPIRATLRRTLCTLLLGAAASLTPAAHANEVFATPEAAMTAFGNAVINDDEPTLQKLFGGKFRDIIPPVGAEVRSKFLTAWGKSHAVDQKENRAYIDVGDDGWTFPIPLVKGSKGWQFDTLAGVEEMRVRRVGRNELAVIQTMLAITDAQAEYALTLHDGSKTLVYASKLSSTPGKQDGLYWASAPGEPQSPLGAAFRGAGTSHASKDGYYGYHYKLLDAQGPNAPGGAYSYVVNGNLFGGFAVLAWPARYQDTGVMSFMVSHDGQVYERDLGADSAVKAKEIRNFDPGAGWRKVAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LCY5</accession>
    <name>Q1LCY5_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Extra-cytoplasmic solute receptor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">bugT</name>
        <name evidence="4" type="ordered locus">Rmet_5132</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="5">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="5">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Periplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the UPF0065 (bug) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11991.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519538.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5132"/>
    <dbReference type="EnsemblBacteria" id="ABF11991">
        <property type="protein sequence ID" value="ABF11991"/>
        <property type="gene ID" value="Rmet_5132"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5132"/>
    <dbReference type="eggNOG" id="COG3181">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045683_0_1_4"/>
    <dbReference type="OMA" id="QTQFGMP"/>
    <dbReference type="OrthoDB" id="447734at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042597">
        <property type="term" value="C:periplasmic space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005064">
        <property type="entry name" value="BUG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042100">
        <property type="entry name" value="Bug_dom1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006311">
        <property type="entry name" value="TAT_signal"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42928">
        <property type="entry name" value="PTHR42928"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03401">
        <property type="entry name" value="TctC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017082">
        <property type="entry name" value="YflP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51318">
        <property type="entry name" value="TAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-0675">Receptor</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193013" evidence="3">
        <location>
            <begin position="30"/>
            <end position="332"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF11991.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="332" mass="34752" checksum="B3E062E2397D89BB" modified="2006-05-30" version="1">MNRRQWFATAGAVAGATALSGMMSRVARAAAPYPSRPIRLIVPFIAGTAPDSMARTISAELAPVLGQPLVVENIGGAGGIIGARALKRAEPDGYTLGVLANQHVINVHLYRDQPYDATKDFTAISALSGGPVVLAVPASSPYKTARDLIEAMRRQPDGLNYGSGGKGSVSHLAVESLLHQTRTKAVHIPYKGASEIVTAMMSGQTQFGMPVLSTAAPFLRNGQLRSLAVSSAARSIYFPDVPTLAESLPPGFVLDNWSGLFAPAGLPAPLTKKLFDAMVALQASGKLDASMKANAGEIRRSASPAEFAQMVAADNARYDKLIGEIGMRGEVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="99" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LD74</accession>
    <name>Q1LD74_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Transcriptional regulator, GntR family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Rmet_5040</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="7">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="7">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11902.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5040"/>
    <dbReference type="EnsemblBacteria" id="ABF11902">
        <property type="protein sequence ID" value="ABF11902"/>
        <property type="gene ID" value="Rmet_5040"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5040"/>
    <dbReference type="eggNOG" id="COG1802">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017584_5_2_4"/>
    <dbReference type="OMA" id="IRWIDME"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.530">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011711">
        <property type="entry name" value="GntR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008920">
        <property type="entry name" value="TF_FadR/GntR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000524">
        <property type="entry name" value="Tscrpt_reg_HTH_GntR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07729">
        <property type="entry name" value="FCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00392">
        <property type="entry name" value="GntR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00035">
        <property type="entry name" value="HTHGNTR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00895">
        <property type="entry name" value="FCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00345">
        <property type="entry name" value="HTH_GNTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48008">
        <property type="entry name" value="SSF48008"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50949">
        <property type="entry name" value="HTH_GNTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0614">Plasmid</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH gntR-type" evidence="5">
        <location>
            <begin position="24"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="9"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABF11902.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="243" mass="27865" checksum="73D970F002C06739" modified="2006-05-30" version="1">MIRAIQESHRAETSMNSPISTTRRKRGSDVFQTLRDMAISYALKPGERLNEIELAEQLGVSRTPVREALARLATHGFLEESSRGYVRRPLDIREMQDLYEARVAVERECLRLAFERASAEGIEELARYLEHSRSVSPDTPAQQLVELDEGFHLRIAELSGNAELRRMLASLNERIRFIRWIDMENVGRDSTQSEHAEIVDALKARDWQRAEARLTSHIALRREQIVDAIKKGLARVYLEGEAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LDH4</accession>
    <name>Q1LDH4_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Ubiquinone biosynthesis protein UbiU</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">ubiU</name>
        <name evidence="2" type="ordered locus">Rmet_4940</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Required for O(2)-independent ubiquinone (coenzyme Q) biosynthesis. Together with UbiV, is essential for the C6-hydroxylation reaction in the oxygen-independent ubiquinone biosynthesis pathway.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; ubiquinone biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Forms an heterodimer with UbiV.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase U32 family. UbiU subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11802.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519362.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4940"/>
    <dbReference type="EnsemblBacteria" id="ABF11802">
        <property type="protein sequence ID" value="ABF11802"/>
        <property type="gene ID" value="Rmet_4940"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4940"/>
    <dbReference type="eggNOG" id="COG0826">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011540_3_2_4"/>
    <dbReference type="OMA" id="GGLCVME"/>
    <dbReference type="UniPathway" id="UPA00232"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008233">
        <property type="term" value="F:peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006744">
        <property type="term" value="P:ubiquinone biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02232">
        <property type="entry name" value="UbiU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001539">
        <property type="entry name" value="Peptidase_U32"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043692">
        <property type="entry name" value="UbiU"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01136">
        <property type="entry name" value="Peptidase_U32"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01276">
        <property type="entry name" value="PEPTIDASE_U32"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0408">Iron</keyword>
    <keyword evidence="1" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0831">Ubiquinone biosynthesis</keyword>
    <feature type="binding site" evidence="1">
        <location>
            <position position="180"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="187"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="204"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="243"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02232"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF11802.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="342" mass="36833" checksum="7845B9F7F3FEF4AB" modified="2006-05-30" version="1">MNPPLAAPVRRPQLVAPAGSLAALRMALQHGADAVYLGLRDATNARNFGGLNFSEDDIRTGVAEAHACGAEVLFAINTFAQMGHVEQWHHAVDAAAALGADAVIMADPGLIAYAAKRHPSLRIHLSVQGSATHADAIELMREQFGIRRVVLPRVLTVTQIAKLARQTDIELEVFGFGSLCVMAEGRCLLSSYATGDSPNNKGVCSPAHAVRWDEQDGTMQARLSGILIDSYAPGEPAGYPTLCKGRFTVEGERGYVLEEPTSLNAVSLLPTLIDIGIAAIKIEGRQRSPRYVADVVGVLRAAIDDACRDPKRFAPRQEWQATLGRHAEGDQVTQGAYERPWR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="63" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LDW3</accession>
    <name>Q1LDW3_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_4801</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11663.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519228.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4801"/>
    <dbReference type="EnsemblBacteria" id="ABF11663">
        <property type="protein sequence ID" value="ABF11663"/>
        <property type="gene ID" value="Rmet_4801"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4801"/>
    <dbReference type="eggNOG" id="COG4104">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_148568_4_2_4"/>
    <dbReference type="OMA" id="PHGINKI"/>
    <dbReference type="OrthoDB" id="1960797at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008727">
        <property type="entry name" value="PAAR_motif"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05488">
        <property type="entry name" value="PAAR_motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF11663.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="88" mass="8973" checksum="4D02DA169FBF805A" modified="2006-05-30" version="1">MTSIAFVGDATTHGGVILTGSDTMIVMGRKAARVGDLVSCPLPGHGINPIIEGSDMLTDNGKAIALHGHRTACGCTLIALSTDATIRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-10-07" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LE42</accession>
    <name>Q1LE42_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Putative lipoprotein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_4722</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11584.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4722"/>
    <dbReference type="EnsemblBacteria" id="ABF11584">
        <property type="protein sequence ID" value="ABF11584"/>
        <property type="gene ID" value="Rmet_4722"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4722"/>
    <dbReference type="HOGENOM" id="CLU_1561621_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF11584.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="171" mass="18751" checksum="B2AB106B39E38783" modified="2006-05-30" version="1">MRGAHGWVLAAVTAATMLGGCATYPGYDSPYGYDPNYPLGDLGSVYGGYPGYEYDYAYPYWYGYPYAYGGGYPYWPAYWPAYTGGVFFGSFWYGCCHTSYYHRYGWYGHGYRGYYGHGWYGYRGGYYGYRGSPGYPGYRGYAGYQGAGGMPGWGGGGRGGRSGGGRGGGHR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="70" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LE86</accession>
    <name>Q1LE86_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_4678</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11540.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519112.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4678"/>
    <dbReference type="EnsemblBacteria" id="ABF11540">
        <property type="protein sequence ID" value="ABF11540"/>
        <property type="gene ID" value="Rmet_4678"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4678"/>
    <dbReference type="eggNOG" id="COG1878">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030671_0_1_4"/>
    <dbReference type="OrthoDB" id="831439at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004061">
        <property type="term" value="F:arylformamidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019441">
        <property type="term" value="P:tryptophan catabolic process to kynurenine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.30.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007325">
        <property type="entry name" value="KFase/CYL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037175">
        <property type="entry name" value="KFase_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04199">
        <property type="entry name" value="Cyclase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102198">
        <property type="entry name" value="SSF102198"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF11540.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="352" mass="38599" checksum="767D821DDA4EB8FD" modified="2006-05-30" version="1">MKRWKQRPEGANWGDFGDDDQLGRLNLITADRVLAAAREIRSGKTFCLSLPLDLPGGNVLNPRRHPPVLRPTFRDDVAYVNFPLAKVDPNALDVLSDDQVLLSMQYSTQWDSLAHVGALFDADGDGVAERVYYNGFRPNTDIVGPVDYAEDDHFSAHPCGHDHSHADALGIETFAAHGIQARGVLVDLAAHYGRECRTVGYDDLMRVIEADGIVVEPGDILVLRTGFAEMVMEMAGQPDAEVLHHSCCALDGRDERLLQWITDSGIAAIAADNYAVERYPARPAPEGAGPVPMLPLHHHCLFKLGLPLGELWYLRDLAAWLRAEGRHHFFLSAPPLRLPGAMGSPVTPVATV</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LEC6</accession>
    <name>Q1LEC6_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative Glycosyl hydrolase, BNR repeat protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_4638</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11500.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519074.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4638"/>
    <dbReference type="EnsemblBacteria" id="ABF11500">
        <property type="protein sequence ID" value="ABF11500"/>
        <property type="gene ID" value="Rmet_4638"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4638"/>
    <dbReference type="eggNOG" id="COG4447">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_058803_2_0_4"/>
    <dbReference type="OMA" id="GQCVHKI"/>
    <dbReference type="OrthoDB" id="1293876at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="78"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF11500.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="377" mass="40927" checksum="69D80CD65019FB82" modified="2006-05-30" version="1">MSDRLLVATRKGLFVLQADGKGGWTLGEPHFIGEPVNMVLADRRDGSLYAALNLGHFGVKLHRRRAGMDDWEECAVPVYPPQPADEQTAGGANDASATAPSPPSPPWSLQQIWSLETGGGDEPGVLWAGTIPGGLFRSDDGGDTWVLNRALWDRPERRGWFGGGYDAPGIHSVMVDPRDSRHVTIGVSCGGVWQTSDGGATWQLTADGMEADYMPPEQRGDANTQDPHRVVQCAANPDVLWTQHHCAIFRSTDGAMQWHRIEAQPSSFGFAVAVHPHEPDTAWFVPAVKDQCRIPVDGQFIVTRTRDGGRTFERFSAGLPSAPAYDLVYRHGLAVDDSGTRLAMGSTTGALWASENGGENWHLISAHLPPIYCVRFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="58" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LEI0</accession>
    <name>Q1LEI0_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_4584</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11446.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008648536.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF11446">
        <property type="protein sequence ID" value="ABF11446"/>
        <property type="gene ID" value="Rmet_4584"/>
    </dbReference>
    <dbReference type="GeneID" id="60825946"/>
    <dbReference type="KEGG" id="rme:Rmet_4584"/>
    <dbReference type="HOGENOM" id="CLU_136209_3_0_4"/>
    <dbReference type="OMA" id="QKFDTYS"/>
    <dbReference type="OrthoDB" id="1960881at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193127" evidence="2">
        <location>
            <begin position="23"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="28"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="57"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF11446.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="99" mass="10383" checksum="04007DC7F7BDA27E" modified="2006-05-30" version="1">MNKLIATAIALGLATASVAAVAQDKKQFDPYSQGAKAGQKFDTYSEGAKSGDKFDPYSQGANKSTRADLTDQSTQSTDTTKKSSKSSKKATKKPTASNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-10-07" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LEL0</accession>
    <name>Q1LEL0_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_4551</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11416.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF11416">
        <property type="protein sequence ID" value="ABF11416"/>
        <property type="gene ID" value="Rmet_4551"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4551"/>
    <dbReference type="HOGENOM" id="CLU_2370739_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="12"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="52"/>
            <end position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF11416.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="95" mass="10073" checksum="9EDDEAE35CD53928" modified="2006-05-30" version="1">MFTESGARHASARFGVLVAILLIAIEIVCAASAILALRSLREVGRHYKAFEDGLSPPAAARDQSRDASASPPNLARHSSEVADVSGPEFRSITLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="103" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LET3</accession>
    <name>Q1LET3_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="9">High-affinity branched-chain amino acid transport protein (ABC superfamily, ATP binding)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">livG3</name>
        <name evidence="9" type="ordered locus">Rmet_4478</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="10">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="10">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11343.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008643515.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4478"/>
    <dbReference type="EnsemblBacteria" id="ABF11343">
        <property type="protein sequence ID" value="ABF11343"/>
        <property type="gene ID" value="Rmet_4478"/>
    </dbReference>
    <dbReference type="GeneID" id="60821769"/>
    <dbReference type="KEGG" id="rme:Rmet_4478"/>
    <dbReference type="eggNOG" id="COG0411">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_2_4"/>
    <dbReference type="OMA" id="IPCFAKR"/>
    <dbReference type="OrthoDB" id="1179151at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032823">
        <property type="entry name" value="BCA_ABC_TP_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12399">
        <property type="entry name" value="BCA_ABC_TP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="1" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-0614">Plasmid</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-1278">Translocase</keyword>
    <feature type="domain" description="ABC transporter" evidence="8">
        <location>
            <begin position="6"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="38"/>
            <end position="45"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ABF11343.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="255" mass="27293" checksum="78E240A75B9F8666" modified="2006-05-30" version="1">MSTPILEATGIVKQYGKFMALGGVDLRVMPGTVHSVIGPNGAGKTTLFHMLTGTRPVSAGRIVFEGKDVTAEADYQRVQRGIARSFQVTSLFPNLSVRENLRLAALGTSPRKAMNGWRLPAGDLACAEVVDHVLERLELTHVSGSAAGVLSHGQQRRLEVGMALAARPRAVFLDEPTSGMGVDDLGAMKRLIRGLVQDHTVVLIEHNMDIVMDISDTITVMQQGKVLMEGAPADVRSDPRVRAAYLGNMITGGKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LET6</accession>
    <name>Q1LET6_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Branched-chain amino acid ABC transporter, periplasmic amino acid-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_4475</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11340.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518939.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4475"/>
    <dbReference type="EnsemblBacteria" id="ABF11340">
        <property type="protein sequence ID" value="ABF11340"/>
        <property type="gene ID" value="Rmet_4475"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4475"/>
    <dbReference type="eggNOG" id="COG0683">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027128_1_0_4"/>
    <dbReference type="OMA" id="GAQYWHT"/>
    <dbReference type="OrthoDB" id="538961at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028081">
        <property type="entry name" value="Leu-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028082">
        <property type="entry name" value="Peripla_BP_I"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13458">
        <property type="entry name" value="Peripla_BP_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53822">
        <property type="entry name" value="SSF53822"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peripla_BP_6" evidence="1">
        <location>
            <begin position="24"/>
            <end position="360"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF11340.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="392" mass="42174" checksum="F9C70DF8517A8634" modified="2006-05-30" version="1">MAAVSAVPGVVTYSRSVLAAGEAIELGCPVPMSGAFAANGKFADLGMKLAVEQYGKVLGRPLQYSVLDTEGKPATAVRKVQELAQQKAARYFAGGILSSESLAMGKEAEKLGGVFVTTAGADEITGKDCNRATFRWSVPTYGAIERTVRPLIEAMPKAKRWYTITPQYVFGDGLLSAAKAIFKEKGIEHVGNSYHALTEKEFSGYLTNAMAAKPDVLLILNFGSQSSDTLRQAVSFGMKKNCTILVAWASGLEQFESLGADLCEGVYFGAQYWHGVDTPLNRDLVKRANEKFKANPNYSLAGSYICTKILVDGMIKAGSADPKAVVAALEGLKYEGLTGPEEIRAGDHQVLKNYYLLKGKAKSKMKDKDDFAEIVSVGKAFLPLDQTQCKMA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LEX5</accession>
    <name>Q1LEX5_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Beta-lactamase</fullName>
            <ecNumber evidence="2">3.5.2.6</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">bla</name>
        <name evidence="4" type="ordered locus">Rmet_4436</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="5">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="5">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-A beta-lactamase family.</text>
    </comment>
    <dbReference type="EC" id="3.5.2.6" evidence="2"/>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11301.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518907.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4436"/>
    <dbReference type="EnsemblBacteria" id="ABF11301">
        <property type="protein sequence ID" value="ABF11301"/>
        <property type="gene ID" value="Rmet_4436"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4436"/>
    <dbReference type="eggNOG" id="COG2367">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031960_9_2_4"/>
    <dbReference type="OMA" id="FVTRFMV"/>
    <dbReference type="OrthoDB" id="991474at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008800">
        <property type="term" value="F:beta-lactamase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030655">
        <property type="term" value="P:beta-lactam antibiotic catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046677">
        <property type="term" value="P:response to antibiotic"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.710.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012338">
        <property type="entry name" value="Beta-lactam/transpept-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045155">
        <property type="entry name" value="Beta-lactam_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000871">
        <property type="entry name" value="Beta-lactam_class-A"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35333">
        <property type="entry name" value="PTHR35333"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13354">
        <property type="entry name" value="Beta-lactamase2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56601">
        <property type="entry name" value="SSF56601"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0614">Plasmid</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Beta-lactamase2" evidence="3">
        <location>
            <begin position="44"/>
            <end position="249"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009009"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF13354"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF11301.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="293" mass="30889" checksum="ED6A75FEF66D7028" modified="2006-05-30" version="1">MELVAFPLGSRIPDAAALRARVETALAPLLAGTQARVAISLRLADGKVVLEREAERVQPSASIIKLPILFTLLEQVAQGHLQLEQRFSLDGLERVGGTGILSQLPSVQSLTLAELVRLMIVLSDNLATNALIELLGFEGVNDWCDRAGLGQTRLQRRMMDAAARAAGLDNFTSASDAAAPLCWLLRDGSLPAPLRDFALGLLADQRERAHFGAALPAGAMLANKTGQLPGLRHDAGILTVGAYSVVLAVLADGFTDARTAQTLHGGDGAQWLAEAARIVAQALAAQQDENGAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="95" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LEY1</accession>
    <name>Q1LEY1_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Betaine aldehyde dehydrogenase, NAD-dependent</fullName>
            <ecNumber evidence="5">1.2.1.8</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">betB</name>
        <name evidence="5" type="ordered locus">Rmet_4430</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="6">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="6">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the aldehyde dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.2.1.8" evidence="5"/>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11295.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518901.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4430"/>
    <dbReference type="EnsemblBacteria" id="ABF11295">
        <property type="protein sequence ID" value="ABF11295"/>
        <property type="gene ID" value="Rmet_4430"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4430"/>
    <dbReference type="eggNOG" id="COG1012">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005391_5_1_4"/>
    <dbReference type="OMA" id="KARLHAH"/>
    <dbReference type="OrthoDB" id="744602at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008802">
        <property type="term" value="F:betaine-aldehyde dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.309.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.605.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016161">
        <property type="entry name" value="Ald_DH/histidinol_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016163">
        <property type="entry name" value="Ald_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029510">
        <property type="entry name" value="Ald_DH_CS_GLU"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016162">
        <property type="entry name" value="Ald_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015590">
        <property type="entry name" value="Aldehyde_DH_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00171">
        <property type="entry name" value="Aldedh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53720">
        <property type="entry name" value="SSF53720"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00687">
        <property type="entry name" value="ALDEHYDE_DEHYDR_GLU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 3 5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-0614">Plasmid</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aldedh" evidence="4">
        <location>
            <begin position="23"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="active site" evidence="2">
        <location>
            <position position="253"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABF11295.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="496" mass="52554" checksum="A88BC8EAE6AA5905" modified="2006-05-30" version="1">MQDQSDQTRALWVDGVSVFTPARTFDSVNPATGEVNWQVAFATAEHVAAAVASAKRAVNDPAWRKMLPHVRAGILMRIADAMTARADEFARLQMLENGKVWSECRAQALSAASTFRFYASACETLGSEVTPSRGNYLSMTAYEPYGVVAAITPWNSPLTMEAQKVAPALAAGNAVILKPSEVTPSGALLLAQVAFEAGLPKGLLNVVTGDGRETGTALVEHPDVRMVSFTGGTATGRSIAGIAAQRLIPVALELGGKSPHIIFEDANIDAAINGVVDGIFEGSGQSCVAGSRLFVQRSIYDKAVGLLVERARALRVGLPDVAGSQLGPLAGFAHRDRVERFVSIAREEGGQILAGGRRPPEAALQAGAFFEPTVIGGLSNSATAVQQEIFGPVLCVLPFGDEDDLIAQANDSAFGLASGIWTANYQRAWRVARALETGLVWINTYKQLSIATPFGGFKDSGIGREKGVNGVRLYQQSKGIYWSMDEKNFPSDGALA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LFB5</accession>
    <name>Q1LFB5_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">DoxX, putative exported protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Rmet_4295</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="8">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="8">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the DoxX family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11161.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4295"/>
    <dbReference type="DNASU" id="4041153"/>
    <dbReference type="EnsemblBacteria" id="ABF11161">
        <property type="protein sequence ID" value="ABF11161"/>
        <property type="gene ID" value="Rmet_4295"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4295"/>
    <dbReference type="eggNOG" id="COG2259">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_058421_8_3_4"/>
    <dbReference type="OMA" id="LLYIMAF"/>
    <dbReference type="OrthoDB" id="2084223at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032808">
        <property type="entry name" value="DoxX"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07681">
        <property type="entry name" value="DoxX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0614">Plasmid</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="51"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="77"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="109"/>
            <end position="128"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABF11161.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="140" mass="14456" checksum="4923BE891846A0D5" modified="2006-05-30" version="1">MNKNLAIPKTVIPAIGRILIAALFLVSGLGKLMAPGATMSYIASLGLPAPLLGYLGALILELAGAVLLVAGYRTRIVALLLATYAVVTALIFHHALADQNQMFHFLKNFAMAGGLLQVAAFGSGAFGLDARRAPASQAIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-10-07" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LFD5</accession>
    <name>Q1LFD5_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Extra-cytoplasmic solute receptor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">bug</name>
        <name evidence="3" type="ordered locus">Rmet_4275</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Periplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the UPF0065 (bug) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11141.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4275"/>
    <dbReference type="EnsemblBacteria" id="ABF11141">
        <property type="protein sequence ID" value="ABF11141"/>
        <property type="gene ID" value="Rmet_4275"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4275"/>
    <dbReference type="eggNOG" id="COG3181">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045683_0_0_4"/>
    <dbReference type="OMA" id="LTNDATF"/>
    <dbReference type="OrthoDB" id="447734at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042597">
        <property type="term" value="C:periplasmic space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005064">
        <property type="entry name" value="BUG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042100">
        <property type="entry name" value="Bug_dom1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42928">
        <property type="entry name" value="PTHR42928"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03401">
        <property type="entry name" value="TctC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017082">
        <property type="entry name" value="YflP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-0675">Receptor</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF11141.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="341" mass="36402" checksum="28AE1271D6359FE0" modified="2006-05-30" version="1">MHAHRTTARFSAAAILLSSRWTGRFAMLGTAWLLGGGVAQAQTYPTQTIRLIVPYAPGGTTDIIARQLAHRMSEKLGQQVIVENKSGANTAIGADLVARSKPDGYTLLLTNDATFVLNPVVLPGLTYNVSRDFIPVATIGYVPLVMAVPASLPVNSMKALTAYAKDRPHALSYGSFGNGSQPHLMGELFNKLAGTDLTHVPYKGSAPAVSDVIGGQILFTFPALPTIQGFLDAGKLKVLAVSGEKRTQALPDVPTFTEAGFGEMNISAVYGVLGPAKLPKPVVAKLNATILEILNDRDFVETKFAAQGMVPMRLTPEQFARYIEDQTRKTTRIAALAGLKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="64" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LFF4</accession>
    <name>Q1LFF4_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Putative transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_4256</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11122.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4256"/>
    <dbReference type="EnsemblBacteria" id="ABF11122">
        <property type="protein sequence ID" value="ABF11122"/>
        <property type="gene ID" value="Rmet_4256"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4256"/>
    <dbReference type="eggNOG" id="COG0583">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2702164_0_0_4"/>
    <dbReference type="OMA" id="FQVIRRH"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF11122.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="73" mass="8423" checksum="C56B12381A6F67B2" modified="2006-05-30" version="1">MLSYCPKPLLVCDAMRGWVQPVRISEQFETSRLGIIGRRNTMRSNAAQCFVECLLEEVRRRSRSAAPGRRRIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="70" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LFI6</accession>
    <name>Q1LFI6_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Extra-cytoplasmic solute receptor protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">bug</name>
        <name evidence="4" type="ordered locus">Rmet_4223</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="5">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="5">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Periplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the UPF0065 (bug) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11090.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518713.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4223"/>
    <dbReference type="EnsemblBacteria" id="ABF11090">
        <property type="protein sequence ID" value="ABF11090"/>
        <property type="gene ID" value="Rmet_4223"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4223"/>
    <dbReference type="eggNOG" id="COG3181">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045683_0_0_4"/>
    <dbReference type="OMA" id="RFESLGM"/>
    <dbReference type="OrthoDB" id="447734at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042597">
        <property type="term" value="C:periplasmic space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005064">
        <property type="entry name" value="BUG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042100">
        <property type="entry name" value="Bug_dom1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42928">
        <property type="entry name" value="PTHR42928"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03401">
        <property type="entry name" value="TctC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017082">
        <property type="entry name" value="YflP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-0675">Receptor</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193140" evidence="3">
        <location>
            <begin position="21"/>
            <end position="317"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF11090.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="317" mass="33406" checksum="85C05A1FB14BEB6C" modified="2006-05-30" version="1">MKTRIAALLAAAIVAPHAFAQGDGYPNKPIKLVVPFTAGSASDTAARIVAEEVRSSIGTIIVDNRPGANGIIGSSYVAKSPADGYTLLLTSSSTHSGIEALFKRPGYDPIKDFVHISRIATIPMVLVTRPDAPYKTAMDMALYAQKNHLRYGYGSGSAQIAAATFSQMANVPSDAIPYKSQPPAVTDLLGGQIDYMVADTSVVMSFLHGGKLDGLAITSPRRLPELPNVPTMAEAGYKNFDLVVWVGLAAPAGTPTAVVERWNKAVNDAVARPPVVEKLAKLGMVAAPASTRDFAAFTVAQRGIWTSRATKAGVEQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LFJ5</accession>
    <name>Q1LFJ5_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative Glyoxylate/hydroxypyruvate reductase 6-phosphogluconate dehydrogenase hydroxyacid dehydrogenase</fullName>
            <ecNumber evidence="3">1.1.1.95</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_4214</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EC" id="1.1.1.95" evidence="3"/>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11081.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518704.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4214"/>
    <dbReference type="EnsemblBacteria" id="ABF11081">
        <property type="protein sequence ID" value="ABF11081"/>
        <property type="gene ID" value="Rmet_4214"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4214"/>
    <dbReference type="eggNOG" id="COG0111">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019796_1_0_4"/>
    <dbReference type="OMA" id="WHHGTDK"/>
    <dbReference type="OrthoDB" id="1435073at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004617">
        <property type="term" value="F:phosphoglycerate dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029753">
        <property type="entry name" value="D-isomer_DH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006140">
        <property type="entry name" value="D-isomer_DH_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02826">
        <property type="entry name" value="2-Hacid_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00671">
        <property type="entry name" value="D_2_HYDROXYACID_DH_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="2-Hacid_dh_C" evidence="2">
        <location>
            <begin position="133"/>
            <end position="309"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF11081.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="341" mass="36491" checksum="2F7CB8B6861241F3" modified="2006-05-30" version="1">MSQTQPKSQVSVQPLRILLSEPTLREHRAAIDLSMDGAPWLPVLAPATDDPKGVDADIAFVSRDVTGLSTKHQILPATQRFYSAMLEAPALRWVHVHSAGADRAVFGQLRQRGVTVTTSSGANAGVVAQTALAGLLALARHFPLLLAAQRKQRWAPLLGTGLPRDVRGQTATIVGWGPIGQQIGAMLRLLGMSVAVVRQQPTPAGEGFETFAFDTLHEVLPRTDWLILACPLTDRTRGLVDGAALRRLPTGARLINVARGEVVDEPALIEALTEGRVAGAYLDVFAHEPLPATSPLWSLPNVIATPHSAGFSDGNAARVVDIFLDNLRRRLAGQAMRNVSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LFL1</accession>
    <name>Q1LFL1_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Transcriptional regulator, PadR family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_4198</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11065.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4198"/>
    <dbReference type="EnsemblBacteria" id="ABF11065">
        <property type="protein sequence ID" value="ABF11065"/>
        <property type="gene ID" value="Rmet_4198"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4198"/>
    <dbReference type="eggNOG" id="COG1695">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_063440_1_0_4"/>
    <dbReference type="OMA" id="PPEIHEA"/>
    <dbReference type="OrthoDB" id="1706427at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005149">
        <property type="entry name" value="Tscrpt_reg_PadR_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03551">
        <property type="entry name" value="PadR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PadR" evidence="2">
        <location>
            <begin position="92"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="20"/>
            <end position="36"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF03551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF11065.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="247" mass="27536" checksum="F77E1E332348042B" modified="2006-05-30" version="1">MRDCFGSDGRGEGRQGHQGRHGGHSGHGHGHGHGHWRGQRHDGLRVLIHAMGRGGSFWSRGGGGDWDGSDGFDGESLRRGRKFSAEDLQLLLLSLLEEKPSHGYELIKALEARTGGFYKPSPGVVYPALTFLEDLGYATVDTEGNKKRYQLADAGRAYLEENRERLEQMLARLQHVARKMDWMRRAMRGEPQREPEQGGWVPEFVTSRMRLKQALALRSGASPDEQRRIAAILNRAADDIESGDQNA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-10-07" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LFM5</accession>
    <name>Q1LFM5_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_4184</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11051.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4184"/>
    <dbReference type="EnsemblBacteria" id="ABF11051">
        <property type="protein sequence ID" value="ABF11051"/>
        <property type="gene ID" value="Rmet_4184"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4184"/>
    <dbReference type="HOGENOM" id="CLU_2275139_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="41"/>
            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF11051.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="102" mass="11748" checksum="D9A9E85B94F149D0" modified="2006-05-30" version="1">MREKCLQLTDVACGRRSVVFLPMAGFDGADRVRGRQWQLRHNRRDRPAQCPRNGKAGTRSTRHPGQWANNLLLRAAYRNHSCGRPHMNHGDPSCTLRRIVYI</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LFT1</accession>
    <name>Q1LFT1_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">DNA damage-inducible mutagenesis protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">imuA</name>
        <name evidence="2" type="ordered locus">Rmet_4128</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10995.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518621.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4128"/>
    <dbReference type="EnsemblBacteria" id="ABF10995">
        <property type="protein sequence ID" value="ABF10995"/>
        <property type="gene ID" value="Rmet_4128"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4128"/>
    <dbReference type="eggNOG" id="COG4544">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_064653_0_0_4"/>
    <dbReference type="OMA" id="SAIWVFR"/>
    <dbReference type="OrthoDB" id="1941503at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="285"/>
            <end position="309"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF10995.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="309" mass="31912" checksum="5689C45B61A26103" modified="2006-05-30" version="1">MTVSSHVTPPTVVAGHAASSSSAPRASVSELEHRYPGLWRAGQLGRAGRLPVCPTGYDALTAELPGGGWPVGAVTELLLAQGGAGELRLLMPALRALAAAGRCIGFVDPPYLPNAAGLAAGLADGGLPARQLYWVRPQAGASVASRRADMAWAAEQMLRSQAFGAVLVWLPAARPEALRRLQVLAQAGDTVVWVLRPIRVLHESSPAMLRLALSPLPGNLLSIVFHKRRGPARDTPLLLPLEGMLHVPRRAGVPAVPTPVALVVPATPAGADHHVPDISDIRGSASHPGVLDRGAPAAPAAGRAAAELA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="99" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LG55</accession>
    <name>Q1LG55_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative aspartate racemase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_4003</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the aspartate/glutamate racemases family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10871.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518511.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4003"/>
    <dbReference type="EnsemblBacteria" id="ABF10871">
        <property type="protein sequence ID" value="ABF10871"/>
        <property type="gene ID" value="Rmet_4003"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4003"/>
    <dbReference type="eggNOG" id="COG1794">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_055360_1_0_4"/>
    <dbReference type="OMA" id="TNTMHLI"/>
    <dbReference type="OrthoDB" id="1359310at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047661">
        <property type="term" value="F:amino-acid racemase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006807">
        <property type="term" value="P:nitrogen compound metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015942">
        <property type="entry name" value="Asp/Glu/hydantoin_racemase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001920">
        <property type="entry name" value="Asp/Glu_race"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018187">
        <property type="entry name" value="Asp/Glu_racemase_AS_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004380">
        <property type="entry name" value="Asp_race"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21198:SF7">
        <property type="entry name" value="PTHR21198:SF7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01177">
        <property type="entry name" value="Asp_Glu_race"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53681">
        <property type="entry name" value="SSF53681"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00035">
        <property type="entry name" value="asp_race"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00923">
        <property type="entry name" value="ASP_GLU_RACEMASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0413">Isomerase</keyword>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF10871.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="242" mass="26708" checksum="4006BF06A9239D1A" modified="2006-05-30" version="1">MKTIGLIGGMSWESSAEYYRLINQGMKARLGGHRNARSVMVTVCFEEVKTLQHAERWEDLGNQMQQAARQVEAGGADFVVLCTNTMHKLAPAIEAVLDVPFLHIVDPTADALRRAGIERVGLLGTRFTMEQDFYRARMEQRHGIQVVIPDQDDRTLVHDVIYDELCHGVVRSESRAVYQRIIESLKAGGAQGVILGCTEITLLIKPEDSALPTFDTTELHAMAAVALAAGEVARWPEALLPG</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="66" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LG92</accession>
    <name>Q1LG92_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">TagA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_3966</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10834.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF10834">
        <property type="protein sequence ID" value="ABF10834"/>
        <property type="gene ID" value="Rmet_3966"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3966"/>
    <dbReference type="eggNOG" id="ENOG502Z8SD">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_344478_0_0_4"/>
    <dbReference type="OrthoDB" id="469334at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022218">
        <property type="entry name" value="TagA_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12561">
        <property type="entry name" value="TagA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TagA" evidence="2">
        <location>
            <begin position="518"/>
            <end position="618"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="45"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="60"/>
            <end position="82"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF10834.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="821" mass="90263" checksum="44EA4D530C1ACC8D" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="63" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LGB1</accession>
    <name>Q1LGB1_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_3947</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10815.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518455.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3947"/>
    <dbReference type="EnsemblBacteria" id="ABF10815">
        <property type="protein sequence ID" value="ABF10815"/>
        <property type="gene ID" value="Rmet_3947"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3947"/>
    <dbReference type="eggNOG" id="COG4786">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_078227_0_0_4"/>
    <dbReference type="OMA" id="HHAVQPD"/>
    <dbReference type="OrthoDB" id="1456527at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021556">
        <property type="entry name" value="DUF2950"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11453">
        <property type="entry name" value="DUF2950"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193470" evidence="2">
        <location>
            <begin position="29"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="262"/>
            <end position="281"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF10815.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="281" mass="30837" checksum="9B1A9DF0C4FEA029" modified="2006-05-30" version="1">MKRQSRFESMLRAGALAMLLSMSVPVLAQHVYPTPDAAADAFGDAVARSDPDALKQVLGANYEKLVPPGLSQNDIYDFLAAWASHHAVQPDGDKAARIEVGGSGWTFPVPLAKRKTGWQFDLAAGEQEMRRRRIGRNELVAMDTLLQLADAQQRYAEQIGNGKYAHQLVSTPGKTNGLYWPSASAENDSPLGPDALAMGPEVPPEEAFYGYRYRIIAPPKGSDAKFAFIAWPARYGDSGIHSFMVDSERRFYERDLGKSTGTRAGAMRAFSPEGWERAADR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="62" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LGL1</accession>
    <name>Q1LGL1_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_3847</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10715.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518362.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3847"/>
    <dbReference type="EnsemblBacteria" id="ABF10715">
        <property type="protein sequence ID" value="ABF10715"/>
        <property type="gene ID" value="Rmet_3847"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3847"/>
    <dbReference type="eggNOG" id="ENOG5032Z85">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_177836_0_0_4"/>
    <dbReference type="OMA" id="DWAVPFD"/>
    <dbReference type="OrthoDB" id="1938485at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.1130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021250">
        <property type="entry name" value="DUF2789"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038086">
        <property type="entry name" value="DUF2789_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10982">
        <property type="entry name" value="DUF2789"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF10715.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="79" mass="8791" checksum="41E90E738D86282C" modified="2006-05-30" version="1">MDAPFHQFSELFAQLGLPSDESDIRAFIAKHSPLPQNVELWDAAFWTPAQASLLRDEIAEDADWAEAVDQLNLALRAPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="54" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LGM7</accession>
    <name>Q1LGM7_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_3829</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10699.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518345.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3829"/>
    <dbReference type="EnsemblBacteria" id="ABF10699">
        <property type="protein sequence ID" value="ABF10699"/>
        <property type="gene ID" value="Rmet_3829"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3829"/>
    <dbReference type="HOGENOM" id="CLU_1460157_0_0_4"/>
    <dbReference type="OMA" id="WMIAYEE"/>
    <dbReference type="OrthoDB" id="1407717at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF10699.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="185" mass="19947" checksum="E829A66F057D52C8" modified="2006-05-30" version="1">MYLDPMAGLQPLPTAYTPSGLRQGSRPGHLPFSDSPCAVLGSSRLPIGHLTVAEVSAQGLVNLTPCELHRAIEAGHFYAEGYDPAGMPTILPAWQFVPPAPSLIMPIIMALRNEPSGEDPFFWMIAYEELNDLTPAEVLCGRLLCTQGSVSIGQVHHLRRSDAERQATVTAALELLPSRLLRAGP</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="98" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LGR3</accession>
    <name>Q1LGR3_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Transcriptional regulator, LysR family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_3793</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the LysR transcriptional regulatory family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10663.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518311.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3793"/>
    <dbReference type="EnsemblBacteria" id="ABF10663">
        <property type="protein sequence ID" value="ABF10663"/>
        <property type="gene ID" value="Rmet_3793"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3793"/>
    <dbReference type="eggNOG" id="COG0583">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039613_6_4_4"/>
    <dbReference type="OMA" id="PNLACRP"/>
    <dbReference type="OrthoDB" id="1439189at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005119">
        <property type="entry name" value="LysR_subst-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000847">
        <property type="entry name" value="Tscrpt_reg_HTH_LysR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00126">
        <property type="entry name" value="HTH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03466">
        <property type="entry name" value="LysR_substrate"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00039">
        <property type="entry name" value="HTHLYSR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50931">
        <property type="entry name" value="HTH_LYSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH lysR-type" evidence="2">
        <location>
            <begin position="1"/>
            <end position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF10663.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="304" mass="33337" checksum="5C9482DCBCEF0353" modified="2006-05-30" version="1">MDLRQLRYFVALAEELHFGRAAARLAITQPPLSFNIAKLEASLGVQLLQRTTREVALTAAGKEIYKEALKILALTANARELAGRAARGEIGAVHVGFVGSAVLTPLGERIRAFGAIHPDVDVVLHELNSFEQIDALQARHIDIGIIHPRVIPNGIASHSLHGEHFICLLPADHPLARDRTIDVRELRHDDFVLFPRHFSPEFHDQIVALCASVGFTPNIRHQVRHMLTIATLVAKRFGVSIVPRSVDVVTLPNLACRPLGKQATPSELVGIWRDDEQGSAVLSMLHALGAPVQQRELANSPRLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LGS9</accession>
    <name>Q1LGS9_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">2,3-dihydroxybenzoate-AMP ligase, Enterobactin synthetase component E, AMP-dependent synthetase and ligase</fullName>
            <ecNumber evidence="3">2.7.7.58</ecNumber>
            <ecNumber evidence="3">6.3.2.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">entE</name>
        <name evidence="3" type="ordered locus">Rmet_3777</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EC" id="2.7.7.58" evidence="3"/>
    <dbReference type="EC" id="6.3.2.-" evidence="3"/>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10647.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518293.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3777"/>
    <dbReference type="EnsemblBacteria" id="ABF10647">
        <property type="protein sequence ID" value="ABF10647"/>
        <property type="gene ID" value="Rmet_3777"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3777"/>
    <dbReference type="eggNOG" id="COG1021">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000022_59_7_4"/>
    <dbReference type="OMA" id="PMSPHDE"/>
    <dbReference type="OrthoDB" id="1015470at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008668">
        <property type="term" value="F:(2,3-dihydroxybenzoyl)adenylate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016874">
        <property type="term" value="F:ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025110">
        <property type="entry name" value="AMP-bd_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045851">
        <property type="entry name" value="AMP-bd_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020845">
        <property type="entry name" value="AMP-binding_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_Synth/Lig"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13193">
        <property type="entry name" value="AMP-binding_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00455">
        <property type="entry name" value="AMP_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0436">Ligase</keyword>
    <keyword evidence="3" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="AMP-binding" evidence="1">
        <location>
            <begin position="42"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="domain" description="AMP-binding_C" evidence="2">
        <location>
            <begin position="462"/>
            <end position="540"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF10647.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="567" mass="63056" checksum="5265AD04A6C6E717" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LHB5</accession>
    <name>Q1LHB5_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Efflux pump membrane transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">acrB</name>
        <name evidence="5" type="ordered locus">Rmet_3589</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 4">Cell inner membrane</location>
            <topology evidence="2 4">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 4">Belongs to the resistance-nodulation-cell division (RND) (TC 2.A.6) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10461.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518004.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3589"/>
    <dbReference type="EnsemblBacteria" id="ABF10461">
        <property type="protein sequence ID" value="ABF10461"/>
        <property type="gene ID" value="Rmet_3589"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3589"/>
    <dbReference type="eggNOG" id="COG0841">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002755_0_2_4"/>
    <dbReference type="OMA" id="EVNSMLM"/>
    <dbReference type="OrthoDB" id="91817at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015562">
        <property type="term" value="F:efflux transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042908">
        <property type="term" value="P:xenobiotic transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2090.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027463">
        <property type="entry name" value="AcrB_DN_DC_subdom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001036">
        <property type="entry name" value="Acrflvin-R"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004764">
        <property type="entry name" value="HAE1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32063">
        <property type="entry name" value="PTHR32063"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00873">
        <property type="entry name" value="ACR_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00702">
        <property type="entry name" value="ACRIFLAVINRP"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82714">
        <property type="entry name" value="SSF82714"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00915">
        <property type="entry name" value="2A0602"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="340"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="366"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="392"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="434"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="470"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="537"/>
            <end position="555"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="873"/>
            <end position="890"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="897"/>
            <end position="917"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="923"/>
            <end position="947"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="972"/>
            <end position="992"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="1004"/>
            <end position="1026"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004429"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU364070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABF10461.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="1050" mass="113222" checksum="8EB6123FAD35A431" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LHN4</accession>
    <name>Q1LHN4_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Enoyl-CoA hydratase/isomerase</fullName>
            <ecNumber evidence="3">4.2.1.17</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_3470</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the enoyl-CoA hydratase/isomerase family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.17" evidence="3"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10342.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3470"/>
    <dbReference type="EnsemblBacteria" id="ABF10342">
        <property type="protein sequence ID" value="ABF10342"/>
        <property type="gene ID" value="Rmet_3470"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3470"/>
    <dbReference type="eggNOG" id="COG1024">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009834_7_3_4"/>
    <dbReference type="OMA" id="SCDMVVC"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004300">
        <property type="term" value="F:enoyl-CoA hydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016853">
        <property type="term" value="F:isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.12.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029045">
        <property type="entry name" value="ClpP/crotonase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001753">
        <property type="entry name" value="Enoyl-CoA_hydra/iso"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014748">
        <property type="entry name" value="Enoyl-CoA_hydra_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00378">
        <property type="entry name" value="ECH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52096">
        <property type="entry name" value="SSF52096"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0413">Isomerase</keyword>
    <keyword evidence="3" id="KW-0456">Lyase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF10342.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="266" mass="28901" checksum="577ADD423D62FABA" modified="2006-05-30" version="1">MEMESGGKAVDPFVLRETHDGVVTLRLNRPQQFNALSEAMLEALRVAFEDVARDDSVRCVVLAAEGKAFCAGHDLREMRGRPDLAYYRTLFAQCSVVMQAIQALPVPVIARVHGIATAAGCQLVASCDLAIASVAAKFAVSGINVGLFCSTPSVALSRNVSTKRAFDLLVTGRFIDAETAADWGLINEAVPEQELDAAVARKAEAILSKSPAAIRYGKAMFYRQRLMELGDAYAFAGDVMARNMMEEDAGEGIDAFLQKRPPRWQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-10-07" version="53" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LIE8</accession>
    <name>Q1LIE8_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_3206</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10078.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3206"/>
    <dbReference type="EnsemblBacteria" id="ABF10078">
        <property type="protein sequence ID" value="ABF10078"/>
        <property type="gene ID" value="Rmet_3206"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3206"/>
    <dbReference type="HOGENOM" id="CLU_2790985_0_0_4"/>
    <dbReference type="OMA" id="CAVYPTP"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193194" evidence="1">
        <location>
            <begin position="21"/>
            <end position="68"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF10078.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="68" mass="7148" checksum="973B0337A5EB1D66" modified="2006-05-30" version="1">MKKILMGLAVAGAAMLGGCAVYPTPYGPAVAPAPIAVAPAPVYVGPPAVVVRPYGGYYGYYGRRYYHW</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="64" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LIF2</accession>
    <name>Q1LIF2_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_3202</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10074.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517683.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3202"/>
    <dbReference type="EnsemblBacteria" id="ABF10074">
        <property type="protein sequence ID" value="ABF10074"/>
        <property type="gene ID" value="Rmet_3202"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3202"/>
    <dbReference type="HOGENOM" id="CLU_2192644_0_0_4"/>
    <dbReference type="OMA" id="ANEGVEN"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193582" evidence="2">
        <location>
            <begin position="19"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="86"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="93"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF10074.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="107" mass="10225" checksum="DF2E2466368C810A" modified="2006-05-30" version="1">MKKLLALLMITAALTACGKKEEAPQTNTDAAMQNAAESAKAAASSAAAAASDAADAAKAAASDAAADVSAAAEKAKADAGSAMSAAKDAAKDAVNSTADKAKDAVNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LIJ6</accession>
    <name>Q1LIJ6_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Periplasmic dipeptide transport protein (ABC-type dipeptide transport system, periplasmic component)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">dppA</name>
        <name evidence="2" type="ordered locus">Rmet_3158</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10030.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517641.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3158"/>
    <dbReference type="EnsemblBacteria" id="ABF10030">
        <property type="protein sequence ID" value="ABF10030"/>
        <property type="gene ID" value="Rmet_3158"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3158"/>
    <dbReference type="eggNOG" id="COG0747">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017028_7_4_4"/>
    <dbReference type="OMA" id="GIKHEWS"/>
    <dbReference type="OrthoDB" id="162819at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039424">
        <property type="entry name" value="SBP_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023765">
        <property type="entry name" value="SBP_5_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000914">
        <property type="entry name" value="SBP_5_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30290">
        <property type="entry name" value="PTHR30290"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00496">
        <property type="entry name" value="SBP_bac_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01040">
        <property type="entry name" value="SBP_BACTERIAL_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SBP_bac_5" evidence="1">
        <location>
            <begin position="77"/>
            <end position="447"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF10030.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="535" mass="59475" checksum="62439947F2D37D68" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="99" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LIV8</accession>
    <name>Q1LIV8_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Fused malic enzyme oxidoreductase phosphotransacetylase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">maeB1</name>
        <name evidence="6" type="ordered locus">Rmet_3046</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09918.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3046"/>
    <dbReference type="EnsemblBacteria" id="ABF09918">
        <property type="protein sequence ID" value="ABF09918"/>
        <property type="gene ID" value="Rmet_3046"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3046"/>
    <dbReference type="eggNOG" id="COG0280">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG0281">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012366_0_0_4"/>
    <dbReference type="OMA" id="NIWVTDL"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016746">
        <property type="term" value="F:acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004471">
        <property type="term" value="F:malate dehydrogenase (decarboxylating) (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05311">
        <property type="entry name" value="NAD_bind_2_malic_enz"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10380">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10750">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10950">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046346">
        <property type="entry name" value="Aminiacid_DH-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032683">
        <property type="entry name" value="Malate_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012301">
        <property type="entry name" value="Malic_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037062">
        <property type="entry name" value="Malic_N_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012302">
        <property type="entry name" value="Malic_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045213">
        <property type="entry name" value="Malic_NAD-bd_bact_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042113">
        <property type="entry name" value="P_AcTrfase_dom1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042112">
        <property type="entry name" value="P_AcTrfase_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002505">
        <property type="entry name" value="PTA_PTB"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12434">
        <property type="entry name" value="Malate_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00390">
        <property type="entry name" value="malic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03949">
        <property type="entry name" value="Malic_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01515">
        <property type="entry name" value="PTA_PTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01274">
        <property type="entry name" value="malic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00919">
        <property type="entry name" value="Malic_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53223">
        <property type="entry name" value="SSF53223"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="malic" evidence="5">
        <location>
            <begin position="121"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="domain" description="Malic_M" evidence="4">
        <location>
            <begin position="266"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="83"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="83"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM01274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABF09918.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="861" mass="93792" checksum="3A48220F5CFBD0D6" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="110" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LJB1</accession>
    <name>Q1LJB1_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="12">ATPase and specificity subunit of ClpA-ClpP ATP-dependent serine protease, chaperone activity</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">clpA</name>
        <name evidence="12" type="ordered locus">Rmet_2892</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Part of a stress-induced multi-chaperone system, it is involved in the recovery of the cell from heat-induced damage, in cooperation with DnaK, DnaJ and GrpE. Acts before DnaK, in the processing of protein aggregates. Protein binding stimulates the ATPase activity; ATP hydrolysis unfolds the denatured protein aggregates, which probably helps expose new hydrophobic binding sites on the surface of ClpB-bound aggregates, contributing to the solubilization and refolding of denatured protein aggregates by DnaK.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the ClpA/ClpB family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09765.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008644941.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2892"/>
    <dbReference type="EnsemblBacteria" id="ABF09765">
        <property type="protein sequence ID" value="ABF09765"/>
        <property type="gene ID" value="Rmet_2892"/>
    </dbReference>
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    <dbReference type="eggNOG" id="COG0542">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005070_4_2_4"/>
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    <dbReference type="OrthoDB" id="44062at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="F:ATP hydrolysis activity"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008233">
        <property type="term" value="F:peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043335">
        <property type="term" value="P:protein unfolding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019489">
        <property type="entry name" value="Clp_ATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036628">
        <property type="entry name" value="Clp_N_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004176">
        <property type="entry name" value="Clp_R_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013461">
        <property type="entry name" value="ClpA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001270">
        <property type="entry name" value="ClpA/B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018368">
        <property type="entry name" value="ClpA/B_CS1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028299">
        <property type="entry name" value="ClpA/B_CS2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041546">
        <property type="entry name" value="ClpA/ClpB_AAA_lid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07724">
        <property type="entry name" value="AAA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17871">
        <property type="entry name" value="AAA_lid_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02861">
        <property type="entry name" value="Clp_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10431">
        <property type="entry name" value="ClpB_D2-small"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00300">
        <property type="entry name" value="CLPPROTEASEA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01086">
        <property type="entry name" value="ClpB_D2-small"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81923">
        <property type="entry name" value="SSF81923"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02639">
        <property type="entry name" value="ClpA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51903">
        <property type="entry name" value="CLP_R"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00870">
        <property type="entry name" value="CLPAB_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00871">
        <property type="entry name" value="CLPAB_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 9" id="KW-0143">Chaperone</keyword>
    <keyword evidence="12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-0645">Protease</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Clp R" evidence="11">
        <location>
            <begin position="1"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="146"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="743"/>
            <end position="765"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="146"/>
            <end position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008675"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00025613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU004432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51903"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ABF09765.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="765" mass="84099" checksum="FCF00E764224744B" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LJI7</accession>
    <name>Q1LJI7_CUPMC</name>
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        <submittedName>
            <fullName evidence="8">Sulfite reductase, beta subunit (Hemoprotein with two 4Fe-4S domains)</fullName>
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        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">cysI</name>
        <name evidence="8" type="ordered locus">Rmet_2816</name>
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    <organism evidence="8 9">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EC" id="1.8.1.2" evidence="8"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09689.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008644114.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2816"/>
    <dbReference type="EnsemblBacteria" id="ABF09689">
        <property type="protein sequence ID" value="ABF09689"/>
        <property type="gene ID" value="Rmet_2816"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2816"/>
    <dbReference type="eggNOG" id="COG0155">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015667_1_0_4"/>
    <dbReference type="OMA" id="IKISGCM"/>
    <dbReference type="OrthoDB" id="1588103at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004783">
        <property type="term" value="F:sulfite reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.413.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005117">
        <property type="entry name" value="NiRdtase/SiRdtase_haem-b_fer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036136">
        <property type="entry name" value="Nit/Sulf_reduc_fer-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006067">
        <property type="entry name" value="NO2/SO3_Rdtase_4Fe4S_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045169">
        <property type="entry name" value="NO2/SO3_Rdtase_4Fe4S_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045854">
        <property type="entry name" value="NO2/SO3_Rdtase_4Fe4S_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006066">
        <property type="entry name" value="NO2/SO3_Rdtase_FeS/sirohaem_BS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11493">
        <property type="entry name" value="PTHR11493"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01077">
        <property type="entry name" value="NIR_SIR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03460">
        <property type="entry name" value="NIR_SIR_ferr"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00397">
        <property type="entry name" value="SIROHAEM"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55124">
        <property type="entry name" value="SSF55124"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56014">
        <property type="entry name" value="SSF56014"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="2" id="KW-0349">Heme</keyword>
    <keyword evidence="4" id="KW-0408">Iron</keyword>
    <keyword evidence="5" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3 8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NIR_SIR_ferr" evidence="7">
        <location>
            <begin position="50"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="domain" description="NIR_SIR" evidence="6">
        <location>
            <begin position="119"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="domain" description="NIR_SIR_ferr" evidence="7">
        <location>
            <begin position="328"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="domain" description="NIR_SIR" evidence="6">
        <location>
            <begin position="452"/>
            <end position="549"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF03460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ABF09689.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="560" mass="62999" checksum="F1BF33F5B60C5C21" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LJP7</accession>
    <name>Q1LJP7_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Sulfur carrier protein FdhD</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3 4" type="primary">fdhD</name>
        <name evidence="4" type="ordered locus">Rmet_2756</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Required for formate dehydrogenase (FDH) activity. Acts as a sulfur carrier protein that transfers sulfur from IscS to the molybdenum cofactor prior to its insertion into FDH.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the FdhD family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09629.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517327.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2756"/>
    <dbReference type="EnsemblBacteria" id="ABF09629">
        <property type="protein sequence ID" value="ABF09629"/>
        <property type="gene ID" value="Rmet_2756"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2756"/>
    <dbReference type="eggNOG" id="COG1526">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_056887_0_0_4"/>
    <dbReference type="OMA" id="SEMVIKT"/>
    <dbReference type="OrthoDB" id="948173at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097163">
        <property type="term" value="F:sulfur carrier activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016783">
        <property type="term" value="F:sulfurtransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006777">
        <property type="term" value="P:Mo-molybdopterin cofactor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00187">
        <property type="entry name" value="FdhD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016193">
        <property type="entry name" value="Cytidine_deaminase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003786">
        <property type="entry name" value="FdhD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30592">
        <property type="entry name" value="PTHR30592"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02634">
        <property type="entry name" value="FdhD-NarQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015626">
        <property type="entry name" value="FdhD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53927">
        <property type="entry name" value="SSF53927"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 3" id="KW-0501">Molybdenum cofactor biosynthesis</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Cysteine persulfide intermediate" evidence="3">
        <location>
            <position position="113"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF09629.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="278" mass="30882" checksum="F7F0BFEA462042A4" modified="2006-05-30" version="1">MSLRPELTHASVPLIEEVAVLDEQGRERPAWLPGERPLTVYLDKRELVTLMTLGGAPEHLVLGYLRNQRLVESIEQIASVQVDWETESVAVTTRSGVDRIEERTARRVVTTGCGQGTVFGSLMDEVDTIALPPDARLDQETLYAIVDTIRLQQSVYKQAGSVHGCALFRGSELLTFVEDVGRHNAVDAIAGWMWLEDMSGADKIFYTTGRLTSEMVIKGAQMGIPFLLSRSGVTQMGYQMAKRVGMTLFARCTGKHFLLYTGRERFRHTPTEALVPAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="62" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LJT5</accession>
    <name>Q1LJT5_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Putative sugar transferase involved in lipopolysaccharide synthesis</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">Rmet_2718</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the bacterial sugar transferase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09591.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517290.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2718"/>
    <dbReference type="EnsemblBacteria" id="ABF09591">
        <property type="protein sequence ID" value="ABF09591"/>
        <property type="gene ID" value="Rmet_2718"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2718"/>
    <dbReference type="eggNOG" id="COG2148">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024920_0_0_4"/>
    <dbReference type="OMA" id="FHIEYSR"/>
    <dbReference type="OrthoDB" id="1759731at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003362">
        <property type="entry name" value="Bact_transf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017475">
        <property type="entry name" value="EPS_sugar_tfrase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02397">
        <property type="entry name" value="Bac_transf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03025">
        <property type="entry name" value="EPS_sugtrans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 10" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="31"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="57"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="92"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="121"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="256"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="domain" description="Bac_transf" evidence="9">
        <location>
            <begin position="254"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF02397"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ABF09591.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="441" mass="49532" checksum="83675C9F02E5468C" modified="2006-05-30" version="1">MSNKQARLTHSQRSPAANTRGSETVRRASRMLSWALVGLVLFGINTVAAEYLHHSGIVTYVFTRTLLWAIVPYLCTFVLLHRSLHLPAIEGNSLVGMSATLPFGLLLIVFAAFHIEYSRGALLVGYITTLVWSGIGYRRFVQNYVPVFGYTDPRTLEQLEEVLAMPGATPPTPTRFEFIHAIEDAVHCDGLMLDRSATADPERTRALARLKLSHVRMYSVERVGEMLTGRVGLDHIDENFLDDYARHYLYGYVKRLVDIAAVLCLAPLALPLSALTALAIRLESTGPVLFRQARVGRFGEPFTMLKFRSMSVQPDATAQFAQRHDPRVTRVGRVIRKYRLDELPQLWNVLTGDMSLIGPRPEQVPMVDMFADTIAYYPYRHLVRPGLSGWAQVQQGYVGTHAETVTKLSYDLYYVKHCSFALDLLIAVKTVRTLLTGYGAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="100" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LJW5</accession>
    <name>Q1LJW5_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Riboflavin biosynthesis protein RibD</fullName>
        </recommendedName>
        <domain>
            <recommendedName>
                <fullName evidence="10">Diaminohydroxyphosphoribosylaminopyrimidine deaminase</fullName>
                <shortName evidence="10">DRAP deaminase</shortName>
                <ecNumber evidence="10">3.5.4.26</ecNumber>
            </recommendedName>
            <alternativeName>
                <fullName evidence="10">Riboflavin-specific deaminase</fullName>
            </alternativeName>
        </domain>
        <domain>
            <recommendedName>
                <fullName evidence="10">5-amino-6-(5-phosphoribosylamino)uracil reductase</fullName>
                <ecNumber evidence="10">1.1.1.193</ecNumber>
            </recommendedName>
            <alternativeName>
                <fullName evidence="10">HTP reductase</fullName>
            </alternativeName>
        </domain>
    </protein>
    <gene>
        <name evidence="15" type="primary">ribD</name>
        <name evidence="15" type="ordered locus">Rmet_2688</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 10">Converts 2,5-diamino-6-(ribosylamino)-4(3h)-pyrimidinone 5'-phosphate into 5-amino-6-(ribosylamino)-2,4(1h,3h)-pyrimidinedione 5'-phosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>2,5-diamino-6-hydroxy-4-(5-phosphoribosylamino)-pyrimidine + H(+) + H2O = 5-amino-6-(5-phospho-D-ribosylamino)uracil + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:21868"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:58453"/>
            <dbReference type="ChEBI" id="CHEBI:58614"/>
            <dbReference type="EC" id="3.5.4.26"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>5-amino-6-(5-phospho-D-ribitylamino)uracil + NADP(+) = 5-amino-6-(5-phospho-D-ribosylamino)uracil + H(+) + NADPH</text>
            <dbReference type="Rhea" id="RHEA:17845"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:58421"/>
            <dbReference type="ChEBI" id="CHEBI:58453"/>
            <dbReference type="EC" id="1.1.1.193"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10 13">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="10 13">Binds 1 zinc ion.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 10">Cofactor biosynthesis; riboflavin biosynthesis; 5-amino-6-(D-ribitylamino)uracil from GTP: step 2/4.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3 10">Cofactor biosynthesis; riboflavin biosynthesis; 5-amino-6-(D-ribitylamino)uracil from GTP: step 3/4.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5 10">In the C-terminal section; belongs to the HTP reductase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 10">In the N-terminal section; belongs to the cytidine and deoxycytidylate deaminase family.</text>
    </comment>
    <dbReference type="EC" id="3.5.4.26" evidence="10"/>
    <dbReference type="EC" id="1.1.1.193" evidence="10"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09561.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517261.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2688"/>
    <dbReference type="EnsemblBacteria" id="ABF09561">
        <property type="protein sequence ID" value="ABF09561"/>
        <property type="gene ID" value="Rmet_2688"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2688"/>
    <dbReference type="eggNOG" id="COG0117">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG1985">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036590_1_2_4"/>
    <dbReference type="OMA" id="PYIILKW"/>
    <dbReference type="OrthoDB" id="1775351at2"/>
    <dbReference type="UniPathway" id="UPA00275">
        <property type="reaction ID" value="UER00401"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008703">
        <property type="term" value="F:5-amino-6-(5-phosphoribosylamino)uracil reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008835">
        <property type="term" value="F:diaminohydroxyphosphoribosylaminopyrimidine deaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009231">
        <property type="term" value="P:riboflavin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.430.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016192">
        <property type="entry name" value="APOBEC/CMP_deaminase_Zn-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002125">
        <property type="entry name" value="CMP_dCMP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016193">
        <property type="entry name" value="Cytidine_deaminase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024072">
        <property type="entry name" value="DHFR-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004794">
        <property type="entry name" value="Eubact_RibD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011549">
        <property type="entry name" value="RibD_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002734">
        <property type="entry name" value="RibDG_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00383">
        <property type="entry name" value="dCMP_cyt_deam_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01872">
        <property type="entry name" value="RibD_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006769">
        <property type="entry name" value="RibD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53597">
        <property type="entry name" value="SSF53597"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53927">
        <property type="entry name" value="SSF53927"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00326">
        <property type="entry name" value="eubact_ribD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00227">
        <property type="entry name" value="ribD_Cterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00903">
        <property type="entry name" value="CYT_DCMP_DEAMINASES_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51747">
        <property type="entry name" value="CYT_DCMP_DEAMINASES_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 15" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7 10 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="10 12" id="KW-0521">NADP</keyword>
    <keyword evidence="10 15" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 10" id="KW-0686">Riboflavin biosynthesis</keyword>
    <keyword evidence="8 10 13" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="CMP/dCMP-type deaminase" evidence="14">
        <location>
            <begin position="4"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="11">
        <location>
            <position position="55"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="53"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="81"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="90"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="160"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="174"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="176"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="190"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="202"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="206"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="210"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="213"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="228"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="302"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="304"/>
            <end position="310"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004910"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00007417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022619"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRNR" id="PIRNR006769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR006769-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR006769-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR006769-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="ABF09561.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="373" mass="40602" checksum="487CCFE2BF35B5D4" modified="2006-05-30" version="1">MFSAADYTAMEQALALAARGMFTTTPNPRVGCVLMKDGVVIGEGYTQPAGQDHAEIQAMKDAISRGHDPAGATAYVTLEPCSHFGRTPPCADALVRAGIARVVAAMEDPNPTVSGRGLQRLRDAGIDVRCGLLEKEARDLNIGFISRMTRGLPWVRVKVAASLDGGTALHDGTSQWITGQPARDDGHAWRARACAILTGIGTVRDDNPALTVRAIPTPRQPQRVLVDSRLEVPLDAQILNRDTGDFAKPVLVFCAIEDKARQRALEARGVEVVVLPNPHGKVELRRMLEELARRGINELHVEAGFKLNGSLIREGCADELLVYLAPKLLGDAQGMFNLPPLSKLEDATEFRWHEVRQIGDDLRLIARRADTQH</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LKI7</accession>
    <name>Q1LKI7_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">SPOR domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Rmet_2462</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09339.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517052.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2462"/>
    <dbReference type="EnsemblBacteria" id="ABF09339">
        <property type="protein sequence ID" value="ABF09339"/>
        <property type="gene ID" value="Rmet_2462"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2462"/>
    <dbReference type="eggNOG" id="COG3147">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_068683_0_0_4"/>
    <dbReference type="OMA" id="RAGPFND"/>
    <dbReference type="OrthoDB" id="1648036at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042834">
        <property type="term" value="F:peptidoglycan binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1070">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007730">
        <property type="entry name" value="SPOR-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036680">
        <property type="entry name" value="SPOR-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05036">
        <property type="entry name" value="SPOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110997">
        <property type="entry name" value="SSF110997"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51724">
        <property type="entry name" value="SPOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="60"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="domain" description="SPOR" evidence="3">
        <location>
            <begin position="167"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="96"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="134"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="136"/>
            <end position="161"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51724"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF09339.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="245" mass="26152" checksum="38793B2F2FECFE19" modified="2006-05-30" version="1">MGLLSLFSSRKGTDPAPERGRRTRAESSAGIGASRRTADEYADDTLDPDFPQKQRARRRLIGAVVLLAAAIIVLPIMFEHKPRPVSEDVAVSVANGQGATQKPKVEARKAEPLPPSANANRNDAALDAGEEIVTAPASDKSDKSDKPKADAKPDTKVADSKPADKPAAAGTKYLIMIGAFSSEERAKNWLAKLKESKVPAYVEKKTLADGERNLLRAGPFTSRDAAEAAEKKVKAIGLTSKIVEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="92" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LKJ0</accession>
    <name>Q1LKJ0_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">O-succinylhomoserine sulfhydrylase</fullName>
            <shortName evidence="2">OSH sulfhydrylase</shortName>
            <shortName evidence="2">OSHS sulfhydrylase</shortName>
            <ecNumber evidence="2">2.5.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">metB</name>
        <name evidence="2" type="synonym">metZ</name>
        <name evidence="5" type="ordered locus">Rmet_2459</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Catalyzes the formation of L-homocysteine from O-succinyl-L-homoserine (OSHS) and hydrogen sulfide.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>hydrogen sulfide + O-succinyl-L-homoserine = L-homocysteine + succinate</text>
            <dbReference type="Rhea" id="RHEA:27826"/>
            <dbReference type="ChEBI" id="CHEBI:29919"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:57661"/>
            <dbReference type="ChEBI" id="CHEBI:58199"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 2 4">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-homocysteine from O-succinyl-L-homoserine: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Homotetramer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the trans-sulfuration enzymes family. MetZ subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.-" evidence="2"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09336.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517050.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2459"/>
    <dbReference type="EnsemblBacteria" id="ABF09336">
        <property type="protein sequence ID" value="ABF09336"/>
        <property type="gene ID" value="Rmet_2459"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2459"/>
    <dbReference type="eggNOG" id="COG0626">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018986_2_0_4"/>
    <dbReference type="OMA" id="THGGIIV"/>
    <dbReference type="OrthoDB" id="637281at2"/>
    <dbReference type="UniPathway" id="UPA00051">
        <property type="reaction ID" value="UER00449"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016765">
        <property type="term" value="F:transferase activity, transferring alkyl or aryl (other than methyl) groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071266">
        <property type="term" value="P:'de novo' L-methionine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071268">
        <property type="term" value="P:homocysteine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019346">
        <property type="term" value="P:transsulfuration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00614">
        <property type="entry name" value="CGS_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02056">
        <property type="entry name" value="MetZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000277">
        <property type="entry name" value="Cys/Met-Metab_PyrdxlP-dep_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006234">
        <property type="entry name" value="O-succ-hSer_sulfhydrylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11808">
        <property type="entry name" value="PTHR11808"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01053">
        <property type="entry name" value="Cys_Met_Meta_PP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001434">
        <property type="entry name" value="CGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="SSF53383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01325">
        <property type="entry name" value="O_suc_HS_sulf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="2" id="KW-0486">Methionine biosynthesis</keyword>
    <keyword evidence="2 3 4" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0808">Transferase</keyword>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="2 3">
        <location>
            <position position="213"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR001434-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU362118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABF09336.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="402" mass="42801" checksum="876AD3753274B0E2" modified="2006-05-30" version="1">MSEPFNPDAYGIDTLGVRAGTLRTGEFMEHSEAMYLTSSFCFNSAAEAAARFANSEEGYTYSRFTNPTVSMFQSRLAALEGAEACMATASGMSAILSVALATLQAGDHLVSSRSIFGSTMTLFNSILAKFGVETTYVDGTDLAAWRAAVKPNTKLFFLETPSNPLTEVSDIAAVADIAHNAGALFVVDNCFCSPALQQPIKFGADVVVHSATKHIDGQGRVLGGAVVGKHDFIMGKVFPFVRTAGPTLSAFNAWVMLKGMETLAIRMERHSQSALAIAEFLESHPAVNRVFHPALKSHPQYEIAQRQQSGGGAIVSFELKGDSPEAMRAAAWRVIDSTKLCSITGNLGDTRTTITHPYTTTHGRVAPEAKAAAGISEGLIRLAVGLESVEDLKADLLRGLGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="70" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LL72</accession>
    <name>Q1LL72_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Hydrolase, isochorismatase family</fullName>
            <ecNumber evidence="2">3.-.-.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_2225</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EC" id="3.-.-.-" evidence="2"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09104.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516932.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2225"/>
    <dbReference type="EnsemblBacteria" id="ABF09104">
        <property type="protein sequence ID" value="ABF09104"/>
        <property type="gene ID" value="Rmet_2225"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2225"/>
    <dbReference type="eggNOG" id="COG1335">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_068979_5_1_4"/>
    <dbReference type="OMA" id="MADNFAT"/>
    <dbReference type="OrthoDB" id="1442962at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.850">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000868">
        <property type="entry name" value="Isochorismatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036380">
        <property type="entry name" value="Isochorismatase-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00857">
        <property type="entry name" value="Isochorismatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52499">
        <property type="entry name" value="SSF52499"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Isochorismatase" evidence="1">
        <location>
            <begin position="27"/>
            <end position="196"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF09104.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="202" mass="20886" checksum="F43AD2FC183B58AE" modified="2006-05-30" version="1">MSVAPVPTTLLDAAGASRQPSAWSDAVLVLVDHQREYVDGKLPLTGMPEAVASCGELLALARRYGSPVIHVVHHGKAGGAFDPAGPHAAIIEGLTPVDGEQSVVKHLPNSFAGTDLADLLVATGKKELIVAGFQTHMCISATVRSALDHGYRVTLVAAACATRDLPDPLGGAPLSAAVLHRVTLAALNDRFATVVADASVWH</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2010-11-02" modified="2022-08-03" version="99" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LLA9</accession>
    <name>FTSH_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">ATP-dependent zinc metalloprotease FtsH</fullName>
            <ecNumber evidence="1">3.4.24.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">ftsH</name>
        <name type="ordered locus">Rmet_2188</name>
    </gene>
    <organism>
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Acts as a processive, ATP-dependent zinc metallopeptidase for both cytoplasmic and membrane proteins. Plays a role in the quality control of integral membrane proteins.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="1">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homohexamer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">In the central section; belongs to the AAA ATPase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">In the C-terminal section; belongs to the peptidase M41 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.24.-" evidence="1"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09067.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q1LLA9"/>
    <dbReference type="SMR" id="Q1LLA9"/>
    <dbReference type="STRING" id="266264.Rmet_2188"/>
    <dbReference type="PRIDE" id="Q1LLA9"/>
    <dbReference type="EnsemblBacteria" id="ABF09067">
        <property type="protein sequence ID" value="ABF09067"/>
        <property type="gene ID" value="Rmet_2188"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2188"/>
    <dbReference type="eggNOG" id="COG0465">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000688_16_2_4"/>
    <dbReference type="OMA" id="QYGMTER"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004176">
        <property type="term" value="F:ATP-dependent peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030163">
        <property type="term" value="P:protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.760">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01458">
        <property type="entry name" value="FtsH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041569">
        <property type="entry name" value="AAA_lid_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005936">
        <property type="entry name" value="FtsH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011546">
        <property type="entry name" value="Pept_M41_FtsH_extracell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000642">
        <property type="entry name" value="Peptidase_M41"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037219">
        <property type="entry name" value="Peptidase_M41-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17862">
        <property type="entry name" value="AAA_lid_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06480">
        <property type="entry name" value="FtsH_ext"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01434">
        <property type="entry name" value="Peptidase_M41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140990">
        <property type="entry name" value="SSF140990"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01241">
        <property type="entry name" value="FtsH_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0997">Cell inner membrane</keyword>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0482">Metalloprotease</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0645">Protease</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <feature type="chain" id="PRO_0000400380" description="ATP-dependent zinc metalloprotease FtsH">
        <location>
            <begin position="1"/>
            <end position="649"/>
        </location>
    </feature>
    <feature type="topological domain" description="Cytoplasmic" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="19"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="topological domain" description="Periplasmic" evidence="1">
        <location>
            <begin position="40"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="116"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="topological domain" description="Cytoplasmic" evidence="1">
        <location>
            <begin position="137"/>
            <end position="649"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="606"/>
            <end position="649"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="433"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="210"/>
            <end position="217"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="432"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="436"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="508"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="649" mass="71260" checksum="8EEF901F3EA5DBE9" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LLE7</accession>
    <name>Q1LLE7_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Flagellin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">fliC1</name>
        <name evidence="6" type="ordered locus">Rmet_2150</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Flagellin is the subunit protein which polymerizes to form the filaments of bacterial flagella.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Secreted</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Bacterial flagellum</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the bacterial flagellin family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09029.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516856.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2150"/>
    <dbReference type="EnsemblBacteria" id="ABF09029">
        <property type="protein sequence ID" value="ABF09029"/>
        <property type="gene ID" value="Rmet_2150"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2150"/>
    <dbReference type="eggNOG" id="COG1344">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011142_7_2_4"/>
    <dbReference type="OMA" id="MEWQARS"/>
    <dbReference type="OrthoDB" id="636786at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009288">
        <property type="term" value="C:bacterial-type flagellum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005198">
        <property type="term" value="F:structural molecule activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001492">
        <property type="entry name" value="Flagellin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046358">
        <property type="entry name" value="Flagellin_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042187">
        <property type="entry name" value="Flagellin_C_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001029">
        <property type="entry name" value="Flagellin_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42792">
        <property type="entry name" value="PTHR42792"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00700">
        <property type="entry name" value="Flagellin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00669">
        <property type="entry name" value="Flagellin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00207">
        <property type="entry name" value="FLAGELLIN"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0975">Bacterial flagellum</keyword>
    <keyword evidence="6" id="KW-0966">Cell projection</keyword>
    <keyword evidence="6" id="KW-0969">Cilium</keyword>
    <keyword evidence="6" id="KW-0282">Flagellum</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <feature type="domain" description="Flagellin_N" evidence="4">
        <location>
            <begin position="5"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="domain" description="Flagellin_C" evidence="5">
        <location>
            <begin position="542"/>
            <end position="626"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005709"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023143"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU362073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00669"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00700"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABF09029.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="629" mass="65078" checksum="2F03A43296C4F68C" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LLJ0</accession>
    <name>Q1LLJ0_CUPMC</name>
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        <recommendedName>
            <fullName evidence="3">HTH cro/C1-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Rmet_2107</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08986.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2107"/>
    <dbReference type="EnsemblBacteria" id="ABF08986">
        <property type="protein sequence ID" value="ABF08986"/>
        <property type="gene ID" value="Rmet_2107"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2107"/>
    <dbReference type="eggNOG" id="COG1426">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047530_3_0_4"/>
    <dbReference type="OMA" id="ADCWTQV"/>
    <dbReference type="OrthoDB" id="2071222at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00093">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001387">
        <property type="entry name" value="Cro/C1-type_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025194">
        <property type="entry name" value="DUF4115"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000048">
        <property type="entry name" value="IQ_motif_EF-hand-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010982">
        <property type="entry name" value="Lambda_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13464">
        <property type="entry name" value="DUF4115"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00530">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47413">
        <property type="entry name" value="SSF47413"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50943">
        <property type="entry name" value="HTH_CROC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50096">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="144"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="domain" description="HTH cro/C1-type" evidence="3">
        <location>
            <begin position="37"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="180"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="238"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="211"/>
            <end position="226"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF08986.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="351" mass="35783" checksum="366BDB13E39C0174" modified="2006-05-30" version="1">MSEQERADSQAATTGHVGGGATDSDREAVAREIGAKLKDAREAQRLSLEDVSARLKVAASKLVAIESGNVSSLPDVTFAKGVMRAYARTLQVNIDGLLGRYHAQAQATPVTGITQRHEGALNQAFDDRKRFGSKGTGGGAGGRWVWLVCVLALIGVGGYFGFDHAKAWVEARSKAAAEAPKPAVAEERAQEQSNNDGTVSAALPPVMTGNDSPAPSETASASAAQSVAVTPAPATMSAGLPMVTGGEAPAEAAKTPAATPPATVPAAQGDSAGAVQIRFSADSWYEVRDRTGKVILGGTAKAGDTVSGGGAGGPYKIILGNVKGVESLTHNGAAVNFQASNRNNVARLTLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="103" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LLL0</accession>
    <name>Q1LLL0_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Transcriptional regulator, luxR family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">narL</name>
        <name evidence="5" type="ordered locus">Rmet_2087</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08966.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008643789.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2087"/>
    <dbReference type="EnsemblBacteria" id="ABF08966">
        <property type="protein sequence ID" value="ABF08966"/>
        <property type="gene ID" value="Rmet_2087"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2087"/>
    <dbReference type="eggNOG" id="COG2197">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_90_10_4"/>
    <dbReference type="OMA" id="YKKRLMQ"/>
    <dbReference type="OrthoDB" id="930905at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06170">
        <property type="entry name" value="LuxR_C_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016032">
        <property type="entry name" value="Sig_transdc_resp-reg_C-effctor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000792">
        <property type="entry name" value="Tscrpt_reg_LuxR_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00196">
        <property type="entry name" value="GerE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00038">
        <property type="entry name" value="HTHLUXR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00421">
        <property type="entry name" value="HTH_LUXR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46894">
        <property type="entry name" value="SSF46894"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00622">
        <property type="entry name" value="HTH_LUXR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50043">
        <property type="entry name" value="HTH_LUXR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Response regulatory" evidence="4">
        <location>
            <begin position="4"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="domain" description="HTH luxR-type" evidence="3">
        <location>
            <begin position="149"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="2">
        <location>
            <position position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABF08966.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="217" mass="23387" checksum="F1C9223182DD8221" modified="2006-05-30" version="1">MTIRILLIDDHTLFRSGVRALLQRQSDFEVVDEAADGVEGIKRAKQHRPDVILLDLNMPGLSGLEALQLLTEDLPECAVIVLTVSEEGEELASALRSGARGYLIKNIETEALTSAIRRAAAGEPVISDTMTAKLVQQFKAPAAAPALLERGDAAPLTARERDIVRGLVRGESNKMIARALGVAESTVKIHVQNILKKLNLASRVQVAVYAVEHGLTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LLS0</accession>
    <name>Q1LLS0_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Multidrug efflux system (MFS family)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">emrA</name>
        <name evidence="5" type="ordered locus">Rmet_2027</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08906.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516742.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2027"/>
    <dbReference type="EnsemblBacteria" id="ABF08906">
        <property type="protein sequence ID" value="ABF08906"/>
        <property type="gene ID" value="Rmet_2027"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2027"/>
    <dbReference type="eggNOG" id="COG1566">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018816_15_0_4"/>
    <dbReference type="OMA" id="KEVQITH"/>
    <dbReference type="OrthoDB" id="1680841at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039562">
        <property type="entry name" value="MFP_biotin_lipoyl_2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13533">
        <property type="entry name" value="Biotin_lipoyl_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="23"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="domain" description="Biotin_lipoyl_2" evidence="4">
        <location>
            <begin position="61"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="390"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="94"/>
            <end position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF13533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABF08906.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="413" mass="43626" checksum="61EAD4F565D80447" modified="2006-05-30" version="1">MSNNQQSASQKPAPTGDSKRKRLLTTLTAAIVVAGVGYGLYWGLYARHFESTDDAYVAGNVVQVTPLVGGTVVSIAADDTQLVTAGQPLIQLDRADTQVAMEQAEAQLAQAVREVRTLYVNNASLAANVALRDADVAKARDDLRRRQAIAGSGAVSSEEISHAQTALKAAESALLASKEQLASNQVLTDQTTVEKHPNVQRAAANLRSAYLSFARSTLPAPVTGYVAKRSVQVGQRVAAGTPLMAVVPLDQVWVDANFKEVQITHMRIGQPVELEADVYGSKVKYQGHVEGFSAGTGAAFSLLPAQNATGNWIKVVQRLPVRIALDAQQLKEHPLRVGLSMNVTVDVRKEEGAAMVTATNARPLQTDVYDKVAQDADELIARIISVNNGGRTPAPAAPGGASAARAAAKPANT</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LLZ0</accession>
    <name>Q1LLZ0_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Gamma-glutamyltransferase 1</fullName>
            <ecNumber evidence="2">2.3.2.2</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_1957</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.3.2.2" evidence="2"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08836.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1957"/>
    <dbReference type="MEROPS" id="T03.001"/>
    <dbReference type="EnsemblBacteria" id="ABF08836">
        <property type="protein sequence ID" value="ABF08836"/>
        <property type="gene ID" value="Rmet_1957"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1957"/>
    <dbReference type="eggNOG" id="COG0405">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014813_0_1_4"/>
    <dbReference type="OMA" id="VCGMGPP"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102953">
        <property type="term" value="F:hypoglycin A gamma-glutamyl transpeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0103068">
        <property type="term" value="F:leukotriene C4 gamma-glutamyl transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.246.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043138">
        <property type="entry name" value="GGT_lsub_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043137">
        <property type="entry name" value="GGT_ssub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="SSF56235"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="125"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="594"/>
            <end position="616"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF08836.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="616" mass="63613" checksum="0DF12FE8510699E1" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LMC0</accession>
    <name>Q1LMC0_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">DNA polymerase III, delta prime subunit</fullName>
            <ecNumber evidence="4">2.7.7.7</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">holB</name>
        <name evidence="4" type="ordered locus">Rmet_1827</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.7.7.7" evidence="4"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08706.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516556.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1827"/>
    <dbReference type="EnsemblBacteria" id="ABF08706">
        <property type="protein sequence ID" value="ABF08706"/>
        <property type="gene ID" value="Rmet_1827"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1827"/>
    <dbReference type="eggNOG" id="COG0470">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006229_4_3_4"/>
    <dbReference type="OMA" id="QRGHAWL"/>
    <dbReference type="OrthoDB" id="1971388at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008408">
        <property type="term" value="F:3'-5' exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003887">
        <property type="term" value="F:DNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004622">
        <property type="entry name" value="DNA_pol_HolB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00678">
        <property type="entry name" value="holB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0235">DNA replication</keyword>
    <keyword evidence="3" id="KW-0239">DNA-directed DNA polymerase</keyword>
    <keyword evidence="1 4" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF08706.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="337" mass="36677" checksum="6E7364FFEE35CAD6" modified="2006-05-30" version="1">MLYPWQRDDWHRLTALRDRLPHALLLHGQQGIGKRDLALHFAQGLLCESALPDGQPCNTCSACHWFGQGNHPDFTVVRPEALEAGAGEAEGDGESGSKKKAPSKIIRMEQVRALIEAVGVGTHRAGLRVVVVYPLDALQTEGANALLKTLEEPPPSTVFLLVTDRIDRVLPTILSRCRQFSMTRPTSADALDWLRGQGVADVEAQLALAGGAPLTALHAAEAEEQPLQRWLVGQLGSAAALDALAAAEQLQKLPVPAVLGTLQRWTYDLLALCLGTGAARYFPKEQTALARCASATDAHRLQAFAARLVGHRRNENHPLAARLVMESVLLDYRQLFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LMP6</accession>
    <name>Q1LMP6_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Response regulator, LuxR-family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_1699</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08580.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516432.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1699"/>
    <dbReference type="EnsemblBacteria" id="ABF08580">
        <property type="protein sequence ID" value="ABF08580"/>
        <property type="gene ID" value="Rmet_1699"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1699"/>
    <dbReference type="eggNOG" id="COG4566">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_69_8_4"/>
    <dbReference type="OMA" id="LITDVQM"/>
    <dbReference type="OrthoDB" id="1630092at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039420">
        <property type="entry name" value="WalR-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48111">
        <property type="entry name" value="PTHR48111"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Response regulatory" evidence="2">
        <location>
            <begin position="7"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="1">
        <location>
            <position position="59"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF08580.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="124" mass="13292" checksum="BF671D115367BF8D" modified="2006-05-30" version="1">MGSSGQFAVVIDDDESVARAISRLLRAAGIAVDTFTSGTVFLDQILSQPAYRPACVILDVSMPHLDGLEVQRRLGGLSLPIIFITAHDVPEARNKALSAGAIGYLRKPFNTQQLIELVRGVMTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="70" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LNB2</accession>
    <name>Q1LNB2_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative DNA-binding transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">ydcN</name>
        <name evidence="3" type="ordered locus">Rmet_1481</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08364.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516230.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1481"/>
    <dbReference type="EnsemblBacteria" id="ABF08364">
        <property type="protein sequence ID" value="ABF08364"/>
        <property type="gene ID" value="Rmet_1481"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1481"/>
    <dbReference type="eggNOG" id="COG1476">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_066192_29_0_4"/>
    <dbReference type="OMA" id="EIDRSYM"/>
    <dbReference type="OrthoDB" id="2063007at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00093">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001387">
        <property type="entry name" value="Cro/C1-type_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010982">
        <property type="entry name" value="Lambda_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01381">
        <property type="entry name" value="HTH_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00530">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47413">
        <property type="entry name" value="SSF47413"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50943">
        <property type="entry name" value="HTH_CROC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH cro/C1-type" evidence="2">
        <location>
            <begin position="29"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF08364.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="97" mass="10332" checksum="53285F6D4F5F98BD" modified="2006-05-30" version="1">MQKRTVQRGRPAGSTTHDAELAHAFGAAVRALRMERGIAQESLAHQAGIERSHMGKIERGEHMPTLAIIFKIAGALECSTAVLMSEAESQLAAAAQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="67" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LNV1</accession>
    <name>Q1LNV1_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">HoxQ protein involved in nickel incorporation into hydrogenase-1 proteins(Belongs to CMGI-2)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">hoxQ</name>
        <name evidence="3" type="ordered locus">Rmet_1290</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the HupH/HyaF family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08175.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516057.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1290"/>
    <dbReference type="EnsemblBacteria" id="ABF08175">
        <property type="protein sequence ID" value="ABF08175"/>
        <property type="gene ID" value="Rmet_1290"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1290"/>
    <dbReference type="eggNOG" id="COG1773">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_079566_0_0_4"/>
    <dbReference type="OMA" id="LMVQESV"/>
    <dbReference type="OrthoDB" id="391242at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.140">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038527">
        <property type="entry name" value="HupH_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006894">
        <property type="entry name" value="HupH_Hydgase_express_prot_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04809">
        <property type="entry name" value="HupH_C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HupH_C" evidence="2">
        <location>
            <begin position="73"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="domain" description="HupH_C" evidence="2">
        <location>
            <begin position="167"/>
            <end position="282"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF04809"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF08175.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="287" mass="31659" checksum="803BEF91845B04C5" modified="2006-05-30" version="1">MKAFPIPVVALGPGTQTEDEHLDYLPMPKDMDTYRAPMLPEPEEIAGRTAAREALAQVLALLDQAAEGAPGGQVGLLSLSPEDRQLVNQVMGEGEASAIVREESSSLEVRIQESVFAGVWRIMSFNGSVLVDDVIEVGPVPMQLRITADEDAESLYRPWRGLLPANVQNAPLLVEEIRDQIARWQPGQPPHVVNLTLLPVTPEDIAYLDHQLGTGRVLLLSRGYGNCRITNTRHPHCWRVVYYNSMDSVILNTVEVVDMPEVAMAAPEDLRDSHERLADVLGYLEQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="69" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LP38</accession>
    <name>Q1LP38_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Thioesterase superfamily</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_1202</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08088.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1202"/>
    <dbReference type="EnsemblBacteria" id="ABF08088">
        <property type="protein sequence ID" value="ABF08088"/>
        <property type="gene ID" value="Rmet_1202"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1202"/>
    <dbReference type="eggNOG" id="COG0824">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_101141_0_1_4"/>
    <dbReference type="OMA" id="NDMYDHM"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029069">
        <property type="entry name" value="HotDog_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006683">
        <property type="entry name" value="Thioestr_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03061">
        <property type="entry name" value="4HBT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54637">
        <property type="entry name" value="SSF54637"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="4HBT" evidence="1">
        <location>
            <begin position="54"/>
            <end position="136"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF08088.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="175" mass="19407" checksum="F608379B793C983A" modified="2006-05-30" version="1">MAASALISSCRASGRCRLSEPFLTPITMKPEPDCRSAYAHFQPITTRWMDNDVYGHVNNVVYYSYFDTVVNTYLIRAGVLDIEAGETIGLVIETQCNYFSSLSFPDTVVAGLRVAKLGNSSVRYEVGLFCNDDDVAAAQGHFVHVYVDRETRRPVPLPQPLRQALEALVIGQQAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="62" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LP57</accession>
    <name>Q1LP57_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Conserved hypothetical signal peptide protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_1183</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08069.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515963.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF08069">
        <property type="protein sequence ID" value="ABF08069"/>
        <property type="gene ID" value="Rmet_1183"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1183"/>
    <dbReference type="eggNOG" id="ENOG5032B7Z">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_136209_0_0_4"/>
    <dbReference type="OMA" id="QKNYTEG"/>
    <dbReference type="OrthoDB" id="1826965at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193655" evidence="2">
        <location>
            <begin position="26"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="29"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="59"/>
            <end position="73"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF08069.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="112" mass="11744" checksum="E1EFF6DA75271DAA" modified="2006-05-30" version="1">MLKKTVPTLVVAALAALGTMQLAHAQKNYTDGGDLYSGAHSKDKANPNKSAAKSGKFDPYTEGAKKSTKSELAPGKKADPYTDGAKSGKFDPYTDGAKSGKFDPYTDGAKKQ</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-12-12" modified="2022-08-03" version="95" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LPI8</accession>
    <name>PYRG_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">CTP synthase</fullName>
            <ecNumber evidence="1">6.3.4.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Cytidine 5'-triphosphate synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Cytidine triphosphate synthetase</fullName>
            <shortName evidence="1">CTP synthetase</shortName>
            <shortName evidence="1">CTPS</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">UTP--ammonia ligase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">pyrG</name>
        <name type="ordered locus">Rmet_1052</name>
    </gene>
    <organism>
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen. Regulates intracellular CTP levels through interactions with the four ribonucleotide triphosphates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + H2O + L-glutamine + UTP = ADP + CTP + 2 H(+) + L-glutamate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:26426"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:46398"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.4.2"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>H2O + L-glutamine = L-glutamate + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:15889"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + NH4(+) + UTP = ADP + CTP + 2 H(+) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:16597"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:46398"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="activity regulation">
        <text evidence="1">Allosterically activated by GTP, when glutamine is the substrate; GTP has no effect on the reaction when ammonia is the substrate. The allosteric effector GTP functions by stabilizing the protein conformation that binds the tetrahedral intermediate(s) formed during glutamine hydrolysis. Inhibited by the product CTP, via allosteric rather than competitive inhibition.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Pyrimidine metabolism; CTP biosynthesis via de novo pathway; CTP from UDP: step 2/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homotetramer.</text>
    </comment>
    <comment type="miscellaneous">
        <text evidence="1">CTPSs have evolved a hybrid strategy for distinguishing between UTP and CTP. The overlapping regions of the product feedback inhibitory and substrate sites recognize a common feature in both compounds, the triphosphate moiety. To differentiate isosteric substrate and product pyrimidine rings, an additional pocket far from the expected kinase/ligase catalytic site, specifically recognizes the cytosine and ribose portions of the product inhibitor.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the CTP synthase family.</text>
    </comment>
    <dbReference type="EC" id="6.3.4.2" evidence="1"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07938.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515844.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q1LPI8"/>
    <dbReference type="SMR" id="Q1LPI8"/>
    <dbReference type="STRING" id="266264.Rmet_1052"/>
    <dbReference type="MEROPS" id="C26.964"/>
    <dbReference type="EnsemblBacteria" id="ABF07938">
        <property type="protein sequence ID" value="ABF07938"/>
        <property type="gene ID" value="Rmet_1052"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1052"/>
    <dbReference type="eggNOG" id="COG0504">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011675_5_0_4"/>
    <dbReference type="OMA" id="EFNNAYR"/>
    <dbReference type="OrthoDB" id="783657at2"/>
    <dbReference type="UniPathway" id="UPA00159">
        <property type="reaction ID" value="UER00277"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003883">
        <property type="term" value="F:CTP synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004359">
        <property type="term" value="F:glutaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044210">
        <property type="term" value="P:'de novo' CTP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006541">
        <property type="term" value="P:glutamine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03113">
        <property type="entry name" value="CTPS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01746">
        <property type="entry name" value="GATase1_CTP_Synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01227">
        <property type="entry name" value="PyrG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004468">
        <property type="entry name" value="CTP_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017456">
        <property type="entry name" value="CTP_synthase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017926">
        <property type="entry name" value="GATASE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033828">
        <property type="entry name" value="GATase1_CTP_Synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11550">
        <property type="entry name" value="PTHR11550"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06418">
        <property type="entry name" value="CTP_synth_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00117">
        <property type="entry name" value="GATase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="SSF52317"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00337">
        <property type="entry name" value="PyrG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51273">
        <property type="entry name" value="GATASE_TYPE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0665">Pyrimidine biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000266192" description="CTP synthase">
        <location>
            <begin position="1"/>
            <end position="549"/>
        </location>
    </feature>
    <feature type="domain" description="Glutamine amidotransferase type-1" evidence="1">
        <location>
            <begin position="295"/>
            <end position="547"/>
        </location>
    </feature>
    <feature type="region of interest" description="Amidoligase domain" evidence="1">
        <location>
            <begin position="1"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile; for glutamine hydrolysis" evidence="1">
        <location>
            <position position="383"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="520"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="522"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="13"/>
        </location>
        <ligand>
            <name>CTP</name>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
            <note>allosteric inhibitor</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="13"/>
        </location>
        <ligand>
            <name>UTP</name>
            <dbReference type="ChEBI" id="CHEBI:46398"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="14"/>
            <end position="19"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="71"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="71"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="144"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="151"/>
            <end position="153"/>
        </location>
        <ligand>
            <name>CTP</name>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
            <note>allosteric inhibitor</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="191"/>
            <end position="196"/>
        </location>
        <ligand>
            <name>CTP</name>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
            <note>allosteric inhibitor</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="191"/>
            <end position="196"/>
        </location>
        <ligand>
            <name>UTP</name>
            <dbReference type="ChEBI" id="CHEBI:46398"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="227"/>
        </location>
        <ligand>
            <name>CTP</name>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
            <note>allosteric inhibitor</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="227"/>
        </location>
        <ligand>
            <name>UTP</name>
            <dbReference type="ChEBI" id="CHEBI:46398"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="356"/>
        </location>
        <ligand>
            <name>L-glutamine</name>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="384"/>
            <end position="387"/>
        </location>
        <ligand>
            <name>L-glutamine</name>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="407"/>
        </location>
        <ligand>
            <name>L-glutamine</name>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="473"/>
        </location>
        <ligand>
            <name>L-glutamine</name>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01227"/>
        </source>
    </evidence>
    <sequence length="549" mass="60704" checksum="395145B2147557F9" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="109" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LPL5</accession>
    <name>Q1LPL5_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Cysteine desulfurase IscS</fullName>
            <ecNumber evidence="10">2.8.1.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10 13" type="primary">iscS</name>
        <name evidence="13" type="ordered locus">Rmet_1025</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Master enzyme that delivers sulfur to a number of partners involved in Fe-S cluster assembly, tRNA modification or cofactor biosynthesis. Catalyzes the removal of elemental sulfur atoms from cysteine to produce alanine. Functions as a sulfur delivery protein for Fe-S cluster synthesis onto IscU, an Fe-S scaffold assembly protein, as well as other S acceptor proteins.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 10">
            <text>[sulfur carrier]-H + L-cysteine = [sulfur carrier]-SH + L-alanine</text>
            <dbReference type="Rhea" id="RHEA:43892"/>
            <dbReference type="Rhea" id="RHEA-COMP:14737"/>
            <dbReference type="Rhea" id="RHEA-COMP:14739"/>
            <dbReference type="ChEBI" id="CHEBI:29917"/>
            <dbReference type="ChEBI" id="CHEBI:35235"/>
            <dbReference type="ChEBI" id="CHEBI:57972"/>
            <dbReference type="ChEBI" id="CHEBI:64428"/>
            <dbReference type="EC" id="2.8.1.7"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 10 11">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="10">Cofactor biosynthesis; iron-sulfur cluster biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Homodimer. Forms a heterotetramer with IscU, interacts with other sulfur acceptors.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. NifS/IscS subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.8.1.7" evidence="10"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07911.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515819.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1025"/>
    <dbReference type="EnsemblBacteria" id="ABF07911">
        <property type="protein sequence ID" value="ABF07911"/>
        <property type="gene ID" value="Rmet_1025"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1025"/>
    <dbReference type="eggNOG" id="COG1104">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003433_0_2_4"/>
    <dbReference type="OMA" id="KGLYWAR"/>
    <dbReference type="OrthoDB" id="839689at2"/>
    <dbReference type="UniPathway" id="UPA00266"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031071">
        <property type="term" value="F:cysteine desulfurase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008483">
        <property type="term" value="F:transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044571">
        <property type="term" value="P:[2Fe-2S] cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00331">
        <property type="entry name" value="Cys_desulf_IscS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000192">
        <property type="entry name" value="Aminotrans_V_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020578">
        <property type="entry name" value="Aminotrans_V_PyrdxlP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010240">
        <property type="entry name" value="Cys_deSase_IscS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016454">
        <property type="entry name" value="Cysteine_dSase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11601:SF34">
        <property type="entry name" value="PTHR11601:SF34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00266">
        <property type="entry name" value="Aminotran_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005572">
        <property type="entry name" value="NifS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="SSF53383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02006">
        <property type="entry name" value="IscS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00595">
        <property type="entry name" value="AA_TRANSFER_CLASS_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 10" id="KW-0001">2Fe-2S</keyword>
    <keyword evidence="4" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="10" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8 10" id="KW-0408">Iron</keyword>
    <keyword evidence="9 10" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="6 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 10" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10 13" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aminotran_5" evidence="12">
        <location>
            <begin position="9"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="region of interest" description="Pyridoxal phosphate binding" evidence="10">
        <location>
            <begin position="77"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="region of interest" description="Pyridoxal phosphate binding" evidence="10">
        <location>
            <begin position="205"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="active site" description="Cysteine persulfide intermediate" evidence="10">
        <location>
            <position position="330"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="157"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="185"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="245"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="binding site" description="via persulfide group" evidence="10">
        <location>
            <position position="330"/>
        </location>
        <ligand>
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
            <note>ligand shared with IscU</note>
        </ligand>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="10">
        <location>
            <position position="208"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001357"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00331"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU004504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ABF07911.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="406" mass="45305" checksum="051B4C09D4A0F4E5" modified="2006-05-30" version="1">MSTTPHFPIYMDYSATTPVDPRVADKMIPYLREQFGNPASRSHAYGWDAERAVEEAREQVAALVDADPREIVWTSGATESDNLAIKGAANFYSGKGKHIITVKTEHKAVLDTTRELERQGFEVTYLDVKDDGLIDMDVFKQAIRPDTILVSVMLVNNEIGVIQDVEQIGEICREKGIIFHCDAAQATGKVTIDLSTLKCDLMSFSAHKTYGPKGIGALYVRRKPRVRIEAQMHGGGHERGMRSGTLAPHQIVGMGEAFRLAREEMATENERIRMLRDRLWNGLSSMEEVYLNGSMEHRVPHNLNVSFNFVEGESLIMAIKDVAVSSGSACTSASLEPSYVLRALGRNDELAHSSIRFTVGRFTTEAEIDYTVELLKNKIGKLRDLSPLWEMFKDGVDLNSIQWAAH</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LPS0</accession>
    <name>Q1LPS0_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Glutathione hydrolase proenzyme</fullName>
            <ecNumber evidence="4">2.3.2.2</ecNumber>
            <ecNumber evidence="4">3.4.19.13</ecNumber>
        </recommendedName>
        <component>
            <recommendedName>
                <fullName evidence="4">Glutathione hydrolase large chain</fullName>
            </recommendedName>
        </component>
        <component>
            <recommendedName>
                <fullName evidence="4">Glutathione hydrolase small chain</fullName>
            </recommendedName>
        </component>
    </protein>
    <gene>
        <name evidence="6" type="primary">ggt</name>
        <name evidence="6" type="ordered locus">Rmet_0970</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="2 4">
            <text>an S-substituted glutathione + H2O = an S-substituted L-cysteinylglycine + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:59468"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:90779"/>
            <dbReference type="ChEBI" id="CHEBI:143103"/>
            <dbReference type="EC" id="3.4.19.13"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 4">
            <text>an alpha-amino acid + an N-terminal (5-L-glutamyl)-[peptide] = 5-L-glutamyl amino acid + N-terminal L-alpha-aminoacyl-[peptide]</text>
            <dbReference type="Rhea" id="RHEA:23904"/>
            <dbReference type="Rhea" id="RHEA-COMP:9780"/>
            <dbReference type="Rhea" id="RHEA-COMP:9795"/>
            <dbReference type="ChEBI" id="CHEBI:77644"/>
            <dbReference type="ChEBI" id="CHEBI:78597"/>
            <dbReference type="ChEBI" id="CHEBI:78599"/>
            <dbReference type="ChEBI" id="CHEBI:78608"/>
            <dbReference type="EC" id="2.3.2.2"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3 4">
            <text>glutathione + H2O = L-cysteinylglycine + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:28807"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:57925"/>
            <dbReference type="ChEBI" id="CHEBI:61694"/>
            <dbReference type="EC" id="3.4.19.13"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="4">Sulfur metabolism; glutathione metabolism.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">This enzyme consists of two polypeptide chains, which are synthesized in precursor form from a single polypeptide.</text>
    </comment>
    <comment type="PTM">
        <text evidence="4">Cleaved by autocatalysis into a large and a small subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the gamma-glutamyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.2" evidence="4"/>
    <dbReference type="EC" id="3.4.19.13" evidence="4"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07856.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0970"/>
    <dbReference type="MEROPS" id="T03.001"/>
    <dbReference type="EnsemblBacteria" id="ABF07856">
        <property type="protein sequence ID" value="ABF07856"/>
        <property type="gene ID" value="Rmet_0970"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0970"/>
    <dbReference type="eggNOG" id="COG0405">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014813_0_1_4"/>
    <dbReference type="OMA" id="LAPGKRC"/>
    <dbReference type="UniPathway" id="UPA00204"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036374">
        <property type="term" value="F:glutathione hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102953">
        <property type="term" value="F:hypoglycin A gamma-glutamyl transpeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0103068">
        <property type="term" value="F:leukotriene C4 gamma-glutamyl transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006750">
        <property type="term" value="P:glutathione biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006751">
        <property type="term" value="P:glutathione catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.246.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043138">
        <property type="entry name" value="GGT_lsub_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000101">
        <property type="entry name" value="GGT_peptidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043137">
        <property type="entry name" value="GGT_ssub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="SSF56235"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00066">
        <property type="entry name" value="g_glut_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="4" id="KW-0317">Glutathione biosynthesis</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0808">Transferase</keyword>
    <keyword evidence="4" id="KW-0865">Zymogen</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="49"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="633"/>
            <end position="653"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="52"/>
            <end position="68"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00001089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU368036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABF07856.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="653" mass="68348" checksum="4EFE33F5AE8BF290" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-07-22" modified="2022-05-25" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LPS7</accession>
    <name>UREF_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Urease accessory protein UreF</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">ureF</name>
        <name type="ordered locus">Rmet_0963</name>
    </gene>
    <organism>
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Required for maturation of urease via the functional incorporation of the urease nickel metallocenter.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">UreD, UreF and UreG form a complex that acts as a GTP-hydrolysis-dependent molecular chaperone, activating the urease apoprotein by helping to assemble the nickel containing metallocenter of UreC. The UreE protein probably delivers the nickel.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the UreF family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07849.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515770.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q1LPS7"/>
    <dbReference type="SMR" id="Q1LPS7"/>
    <dbReference type="STRING" id="266264.Rmet_0963"/>
    <dbReference type="EnsemblBacteria" id="ABF07849">
        <property type="protein sequence ID" value="ABF07849"/>
        <property type="gene ID" value="Rmet_0963"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0963"/>
    <dbReference type="eggNOG" id="COG0830">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_049215_2_1_4"/>
    <dbReference type="OMA" id="TQYTRLF"/>
    <dbReference type="OrthoDB" id="1942614at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016151">
        <property type="term" value="F:nickel cation binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006807">
        <property type="term" value="P:nitrogen compound metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.4190.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01385">
        <property type="entry name" value="UreF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002639">
        <property type="entry name" value="UreF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038277">
        <property type="entry name" value="UreF_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01730">
        <property type="entry name" value="UreF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF009467">
        <property type="entry name" value="Ureas_acces_UreF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0143">Chaperone</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0996">Nickel insertion</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000344161" description="Urease accessory protein UreF">
        <location>
            <begin position="1"/>
            <end position="230"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01385"/>
        </source>
    </evidence>
    <sequence length="230" mass="25327" checksum="3B80F772EFCDA9AB" modified="2006-05-30" version="1">MTALPQLISLLHLASPALPIGGFSYSQGLEAAIDCELVRDATTAERWIRDNLLHVQAQCEAPVWLLLHRAWQTQDHASVRQWNDWFHATRETSELRLETEQMGWSLAKLIAQMGWGDDASRDLLRDMRPVCLPTAFSSACVALGIAAREGLAAYLFNWAENQVAAAIKAVPLGQVAGQQMLLGLHQAVLGTVDEAVCRADAVPPLLSTFSPMLGVLSARHETQYSRLFRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="109" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LPV7</accession>
    <name>Q1LPV7_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">NADH-quinone oxidoreductase</fullName>
            <ecNumber evidence="10">7.1.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">nuoG</name>
        <name evidence="14" type="ordered locus">Rmet_0933</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 10">
            <text>a quinone + 5 H(+)(in) + NADH = a quinol + 4 H(+)(out) + NAD(+)</text>
            <dbReference type="Rhea" id="RHEA:57888"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:24646"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:132124"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>2Fe-2S cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49601"/>
        </cofactor>
        <text evidence="10">Binds 1 [2Fe-2S] cluster per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 10">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the complex I 75 kDa subunit family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the prokaryotic molybdopterin-containing oxidoreductase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="7.1.1.-" evidence="10"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07819.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515750.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0933"/>
    <dbReference type="EnsemblBacteria" id="ABF07819">
        <property type="protein sequence ID" value="ABF07819"/>
        <property type="gene ID" value="Rmet_0933"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0933"/>
    <dbReference type="eggNOG" id="COG1034">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000422_11_6_4"/>
    <dbReference type="OMA" id="HKNVGPL"/>
    <dbReference type="OrthoDB" id="323168at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008137">
        <property type="term" value="F:NADH dehydrogenase (ubiquinone) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048038">
        <property type="term" value="F:quinone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042773">
        <property type="term" value="P:ATP synthesis coupled electron transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00207">
        <property type="entry name" value="fer2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036010">
        <property type="entry name" value="2Fe-2S_ferredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001041">
        <property type="entry name" value="2Fe-2S_ferredoxin-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009010">
        <property type="entry name" value="Asp_de-COase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006656">
        <property type="entry name" value="Mopterin_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006963">
        <property type="entry name" value="Mopterin_OxRdtase_4Fe-4S_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000283">
        <property type="entry name" value="NADH_UbQ_OxRdtase_75kDa_su_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010228">
        <property type="entry name" value="NADH_UbQ_OxRdtase_Gsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019574">
        <property type="entry name" value="NADH_UbQ_OxRdtase_Gsu_4Fe4S-bd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00384">
        <property type="entry name" value="Molybdopterin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10588">
        <property type="entry name" value="NADH-G_4Fe-4S_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00929">
        <property type="entry name" value="NADH-G_4Fe-4S_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50692">
        <property type="entry name" value="SSF50692"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54292">
        <property type="entry name" value="SSF54292"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01973">
        <property type="entry name" value="NuoG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51085">
        <property type="entry name" value="2FE2S_FER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51839">
        <property type="entry name" value="4FE4S_HC3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51669">
        <property type="entry name" value="4FE4S_MOW_BIS_MGD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00641">
        <property type="entry name" value="COMPLEX1_75K_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00642">
        <property type="entry name" value="COMPLEX1_75K_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00643">
        <property type="entry name" value="COMPLEX1_75K_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0001">2Fe-2S</keyword>
    <keyword evidence="5 10" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="7 10" id="KW-0408">Iron</keyword>
    <keyword evidence="8 10" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="6 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0520">NAD</keyword>
    <keyword evidence="14" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-0874">Quinone</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-1278">Translocase</keyword>
    <feature type="domain" description="2Fe-2S ferredoxin-type" evidence="11">
        <location>
            <begin position="1"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S His(Cys)3-ligated-type" evidence="13">
        <location>
            <begin position="78"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S Mo/W bis-MGD-type" evidence="12">
        <location>
            <begin position="216"/>
            <end position="272"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000100"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010312"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU003525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51669"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ABF07819.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="790" mass="83737" checksum="3E286095619E5E0E" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LQB9</accession>
    <name>Q1LQB9_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative GCN5-related N-acetyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">phnO</name>
        <name evidence="2" type="ordered locus">Rmet_0771</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07657.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515612.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0771"/>
    <dbReference type="EnsemblBacteria" id="ABF07657">
        <property type="protein sequence ID" value="ABF07657"/>
        <property type="gene ID" value="Rmet_0771"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0771"/>
    <dbReference type="eggNOG" id="COG0456">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013985_34_6_4"/>
    <dbReference type="OMA" id="SSAMIIE"/>
    <dbReference type="OrthoDB" id="2055010at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008080">
        <property type="term" value="F:N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00583">
        <property type="entry name" value="Acetyltransf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="SSF55729"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="1">
        <location>
            <begin position="15"/>
            <end position="155"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF07657.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="155" mass="17263" checksum="E0DEA1AF8C41E0FE" modified="2006-05-30" version="1">MSEADFSTALMLREVQCVDAELLHLSALLATMDNETPLPLATMRERFAAMRRYPDYRVYLMFDADGVPLGTFSLLVFPVMVHDGRPEAILEAVVVAPAARGRGIGRQMMQEAMRLAREAGAAKLMLSSNARRLQAHQFYRQLGFTEHGISFSTAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="61" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LQE4</accession>
    <name>Q1LQE4_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_0746</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07632.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515588.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF07632">
        <property type="protein sequence ID" value="ABF07632"/>
        <property type="gene ID" value="Rmet_0746"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0746"/>
    <dbReference type="HOGENOM" id="CLU_1924090_0_0_4"/>
    <dbReference type="OMA" id="HAQVAFW"/>
    <dbReference type="OrthoDB" id="2041128at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193340" evidence="2">
        <location>
            <begin position="30"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="72"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="72"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF07632.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="137" mass="14702" checksum="8D969ECD403C62E5" modified="2006-05-30" version="1">MFTRPLLRLMVAGSVAVAGLGGSIAVSHAQVAFWHHSRTDRTVQAVHAANGWEIASVRAENRDHVQAQIDRMEARARNDDRLSGRPASPGGRDDEQRARQPGGGDRNSPGEATLRNSEAHPGNVGPGWQARGRDGGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LQF4</accession>
    <name>Q1LQF4_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">DL-methionine transporter subunit periplasmic-binding component of ABC superfamily</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">metQ</name>
        <name evidence="6" type="ordered locus">Rmet_0736</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NlpA lipoprotein family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07622.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008643677.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0736"/>
    <dbReference type="EnsemblBacteria" id="ABF07622">
        <property type="protein sequence ID" value="ABF07622"/>
        <property type="gene ID" value="Rmet_0736"/>
    </dbReference>
    <dbReference type="GeneID" id="60825223"/>
    <dbReference type="KEGG" id="rme:Rmet_0736"/>
    <dbReference type="eggNOG" id="COG1464">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_067080_0_0_4"/>
    <dbReference type="OMA" id="HIEPMGV"/>
    <dbReference type="OrthoDB" id="1230456at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004872">
        <property type="entry name" value="Lipoprotein_NlpA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30429">
        <property type="entry name" value="PTHR30429"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03180">
        <property type="entry name" value="Lipoprotein_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002854">
        <property type="entry name" value="MetQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00363">
        <property type="entry name" value="TIGR00363"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193749" evidence="5">
        <location>
            <begin position="25"/>
            <end position="264"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008973"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABF07622.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="264" mass="28428" checksum="74192E041D809C24" modified="2006-05-30" version="1">MQRRNVLKGAIVALGVVVSAGAFAQEKPIKVGVTGGPHAQIMEQVKKVAAKDGLTIQVVEFSDYIQPNAALAAGDLDANSYQHLPYLEAQIKDRGYKFTNVAFTVTFPMGVYSKKIKSLDQLKQGARVGVPNDPTNGGRGLLLLQSKGLIKLRPDAGLKATPLDVVENPKKIKLVELDAAQLPRSLDDLDAAAINGNYAESAGLSPTRDAIAMEGPKGPYANLIAIREADKNKPWVAKLVKAYHSPEIKQYIQATFKDSVITAW</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-10-07" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LQK2</accession>
    <name>Q1LQK2_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_0688</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07574.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF07574">
        <property type="protein sequence ID" value="ABF07574"/>
        <property type="gene ID" value="Rmet_0688"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0688"/>
    <dbReference type="HOGENOM" id="CLU_2668421_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF07574.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="75" mass="8966" checksum="873A5388628CBC3F" modified="2006-05-30" version="1">MRPTMTDTRRWCRPQEETVAMAITTRIRKKKQYRKAVLKLRAAIKRGDAEAIRIRREQLQAMPKPRLRYAREAQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LQN0</accession>
    <name>Q1LQN0_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Transcriptional regulator, LysR-family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_0660</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the LysR transcriptional regulatory family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07546.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515517.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0660"/>
    <dbReference type="EnsemblBacteria" id="ABF07546">
        <property type="protein sequence ID" value="ABF07546"/>
        <property type="gene ID" value="Rmet_0660"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0660"/>
    <dbReference type="eggNOG" id="COG0583">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039613_37_1_4"/>
    <dbReference type="OMA" id="WHAHLAI"/>
    <dbReference type="OrthoDB" id="846433at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005119">
        <property type="entry name" value="LysR_subst-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000847">
        <property type="entry name" value="Tscrpt_reg_HTH_LysR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00126">
        <property type="entry name" value="HTH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03466">
        <property type="entry name" value="LysR_substrate"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00039">
        <property type="entry name" value="HTHLYSR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50931">
        <property type="entry name" value="HTH_LYSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH lysR-type" evidence="2">
        <location>
            <begin position="16"/>
            <end position="73"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF07546.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="313" mass="34533" checksum="D1C7A43B0DBD2C3C" modified="2006-05-30" version="1">MTDSRPPRHSRSPHLPPLQALRALEAATRHRSFTRAAEELALTHSAISHHIRALENNLGTKLFQRTGLSMTPTSAGTRLAEQVRGALDQLESALREAGSVSGPPVVQLQVSVMADLANAWLIRRLPSLHAQAPELDLHLRLHAEISPPDPYAVDVGIWHQRVDLPGFECHNLIEDYVIAVASPALLARYPGFTVADLPRLPMLRFALRPWRDWLLLAGLPPDEPVRGPIFQDAGLMLQSAVAGLGVATARAQLAHDYIASGQLVQIGTTRIPSSLHYWVTWREGNPREKAILRFHTWLKDQIAGIAPENDAQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LQP2</accession>
    <name>Q1LQP2_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Flp pilus assembly CpaB</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">cpaB1</name>
        <name evidence="2" type="ordered locus">Rmet_0648</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07534.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515505.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0648"/>
    <dbReference type="EnsemblBacteria" id="ABF07534">
        <property type="protein sequence ID" value="ABF07534"/>
        <property type="gene ID" value="Rmet_0648"/>
    </dbReference>
    <dbReference type="GeneID" id="60825126"/>
    <dbReference type="KEGG" id="rme:Rmet_0648"/>
    <dbReference type="eggNOG" id="COG3745">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_057068_4_0_4"/>
    <dbReference type="OMA" id="VRAVNWP"/>
    <dbReference type="OrthoDB" id="2060643at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017592">
        <property type="entry name" value="Pilus_assmbl_Flp-typ_CpaB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031571">
        <property type="entry name" value="RcpC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013974">
        <property type="entry name" value="SAF"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16976">
        <property type="entry name" value="RcpC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08666">
        <property type="entry name" value="SAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00858">
        <property type="entry name" value="SAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03177">
        <property type="entry name" value="pilus_cpaB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SAF" evidence="1">
        <location>
            <begin position="37"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00858"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF07534.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="281" mass="29011" checksum="8297148DE546C480" modified="2006-05-30" version="1">MKNTRAILMLLIALVAGAAAVVSASRWLLQQSSSSVKQVVVAANDLDLGQPLNGSQLKLVSWPAGSVPPGAFEDPKTLDGRVVRTSLQRGEPIIDAKLAPVGTKGGLSAVINDGKRAITVRVNDVVGVAGFALPGNYVDVIVNTNEDTKSAQNGSISKIVLEKILVLAVAQQVSRDDTQPKVVNAVTLEVSPDQAEKLDVARSVGTLSLVLRNQMDVADINTGGATKHTLLGKAELPAPAPLAAPAPVTRTVVKTRTVAAAPRAGNCVGVLAGIQGSLECF</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LQV7</accession>
    <name>Q1LQV7_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">CBS-domain-containing membrane protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Rmet_0583</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07469.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515443.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0583"/>
    <dbReference type="EnsemblBacteria" id="ABF07469">
        <property type="protein sequence ID" value="ABF07469"/>
        <property type="gene ID" value="Rmet_0583"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0583"/>
    <dbReference type="eggNOG" id="COG3448">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_040397_1_1_4"/>
    <dbReference type="OMA" id="TRCLHPP"/>
    <dbReference type="OrthoDB" id="2008563at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.580.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007065">
        <property type="entry name" value="HPP"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00571">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04982">
        <property type="entry name" value="HPP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00116">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="SSF54631"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51371">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0129">CBS domain</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="58"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="82"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="109"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="146"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="3">
        <location>
            <begin position="253"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="3">
        <location>
            <begin position="318"/>
            <end position="374"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF07469.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="376" mass="40428" checksum="181C96A2CCF21D8F" modified="2006-05-30" version="1">MPAAFDLSTSVAGTRAWLRTFVPMPIAASRRERIKSCIGALIGLFLTEWICRHFLQGFNPWFVAPMGASAVLLFAVPASPLAQPWSIIGGNLVAATVGVACVRWIPEPGLAAGVAVAVAIALMFQLRCVHPPSGAVAITAVFGGPAVQALGFGFVVAPVAINSALLLLTALAFNNLMQRRYPHRPPEPPSQHLTKDVPPSRRVGFTRADLDAVLAARGEFLDIEEDDLEAILVAAELRAYSRRFGDVRCSDIMSRDVVAVRPGQRAADAAALLARHHIKALPVVDGNRRLLGIVTQSDFFRAHRSNSRRVNGTVRDLMTRVVVTAHVDQPMVELAHAFSDGGLHHVPVVDGDRHVVGMVTQSDLVAALLRRDTTAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LQX9</accession>
    <name>Q1LQX9_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Putative tyrosine/serine phosphatase</fullName>
            <ecNumber evidence="1">3.1.3.48</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_0561</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EC" id="3.1.3.48" evidence="1"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07447.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0561"/>
    <dbReference type="EnsemblBacteria" id="ABF07447">
        <property type="protein sequence ID" value="ABF07447"/>
        <property type="gene ID" value="Rmet_0561"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0561"/>
    <dbReference type="eggNOG" id="COG2365">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_057546_0_0_4"/>
    <dbReference type="OMA" id="HTIFDFR"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004725">
        <property type="term" value="F:protein tyrosine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026893">
        <property type="entry name" value="Tyr/Ser_Pase_IphP-type"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13350">
        <property type="entry name" value="Y_phosphatase3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="SSF52799"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF07447.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="279" mass="31209" checksum="E4C2D38DE5509F23" modified="2006-05-30" version="1">MRRSEIIEADAERFWKACLTARLCEMTNNMNHDTRPSLGLETIPNARDLGGLAGLSGRRVRSGKLYRSANPALASAADLDRLHSLDLDIVVDFRSPGEKSPAEAAFGERFHWLAVPVLEGSMAMDVLVPRLRASTPTQMRDFMLDVYRDFPVRYREAFGAFLRHAEAGRTLFYHCTAGKDRTGFASLLLLSALGVSQDEIVANYLESNHWNRRLNEDVLARVAPIGVDADVIMPLLEVRPEYLEASMDAIARAYGSVEQFLADDLRIDVGQLRGHYLED</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="107" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LQZ5</accession>
    <name>Q1LQZ5_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Alcohol dehydrogenase, zinc-containing</fullName>
            <ecNumber evidence="4">1.1.1.284</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Rmet_0545</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the zinc-containing alcohol dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.284" evidence="4"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07431.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515407.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0545"/>
    <dbReference type="EnsemblBacteria" id="ABF07431">
        <property type="protein sequence ID" value="ABF07431"/>
        <property type="gene ID" value="Rmet_0545"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0545"/>
    <dbReference type="eggNOG" id="COG1062">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026673_14_1_4"/>
    <dbReference type="OMA" id="AGWCQTD"/>
    <dbReference type="OrthoDB" id="1859760at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106322">
        <property type="term" value="F:S-(hydroxymethyl)glutathione dehydrogenase NAD activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106321">
        <property type="term" value="F:S-(hydroxymethyl)glutathione dehydrogenase NADP activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013149">
        <property type="entry name" value="ADH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013154">
        <property type="entry name" value="ADH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002328">
        <property type="entry name" value="ADH_Zn_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020843">
        <property type="entry name" value="PKS_ER"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08240">
        <property type="entry name" value="ADH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00107">
        <property type="entry name" value="ADH_zinc_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00829">
        <property type="entry name" value="PKS_ER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="SSF50129"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00059">
        <property type="entry name" value="ADH_ZINC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1 4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="PKS_ER" evidence="3">
        <location>
            <begin position="10"/>
            <end position="357"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU361277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF07431.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="365" mass="37942" checksum="30CA8A5E25D48753" modified="2006-05-30" version="1">MKAAVMEKAGAPLVIHDVAIAEPGPREVRVRTAAVGLCHSDLHMMQGTYPLPLPAVLGHEVSGVVEAVGTQVSGLKAGDHVVGCLSVYCGHCSVCASGHQVLCQNPEVKLPPGKANRLRLAGNPLSQVFNLSGFAEEILVHENALVKIRDDMPLDRAALLGCAVITGTGAVFHSAKVPPGSTVVVVGCGGVGLSVINGAVIAGASRVIAVDMLPAKLELARKFGATDVVNAGDGDPVAQVMQLTGGGAEFAFECIGLKQTAEQCYAMLAPGGTATVIGLFRPDARIELPALDFLKEKRIQGSFMGSNRLSLDIPRLVELYMQGKLLLDDLISQRISLEDINEGFIAMQSGSVARSVITFPGVGGL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-01-15" modified="2022-05-25" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LR36</accession>
    <name>PUR7_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Phosphoribosylaminoimidazole-succinocarboxamide synthase</fullName>
            <ecNumber evidence="1">6.3.2.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">SAICAR synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">purC</name>
        <name type="ordered locus">Rmet_0504</name>
    </gene>
    <organism>
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate + ATP + L-aspartate = (2S)-2-[5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamido]succinate + ADP + 2 H(+) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:22628"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58443"/>
            <dbReference type="ChEBI" id="CHEBI:77657"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.2.6"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide from 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate: step 1/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the SAICAR synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.3.2.6" evidence="1"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07390.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008652147.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q1LR36"/>
    <dbReference type="SMR" id="Q1LR36"/>
    <dbReference type="STRING" id="266264.Rmet_0504"/>
    <dbReference type="EnsemblBacteria" id="ABF07390">
        <property type="protein sequence ID" value="ABF07390"/>
        <property type="gene ID" value="Rmet_0504"/>
    </dbReference>
    <dbReference type="GeneID" id="60824101"/>
    <dbReference type="KEGG" id="rme:Rmet_0504"/>
    <dbReference type="eggNOG" id="COG0152">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045637_0_0_4"/>
    <dbReference type="OMA" id="TKFEFGF"/>
    <dbReference type="OrthoDB" id="1345271at2"/>
    <dbReference type="UniPathway" id="UPA00074">
        <property type="reaction ID" value="UER00131"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004639">
        <property type="term" value="F:phosphoribosylaminoimidazolesuccinocarboxamide synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00137">
        <property type="entry name" value="SAICAR_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028923">
        <property type="entry name" value="SAICAR_synt/ADE2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001636">
        <property type="entry name" value="SAICAR_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018236">
        <property type="entry name" value="SAICAR_synthetase_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01259">
        <property type="entry name" value="SAICAR_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00081">
        <property type="entry name" value="purC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01057">
        <property type="entry name" value="SAICAR_SYNTHETASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01058">
        <property type="entry name" value="SAICAR_SYNTHETASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0658">Purine biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_1000018767" description="Phosphoribosylaminoimidazole-succinocarboxamide synthase">
        <location>
            <begin position="1"/>
            <end position="302"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00137"/>
        </source>
    </evidence>
    <sequence length="302" mass="33240" checksum="0171AD9A48623014" modified="2006-05-30" version="1">MSDALYESSIQSLPLLGKGKVRDNYAVGDDKLLIVTTDRLSAFDVILGEPIPAKGRVLNQMANFWFKKLAHIVPNHETDVTAESVVAPNEVEQVKGRAVVVKRLKPILVEAVVRGYLAGSGWKDYQATGKVCGIALPAGLQNAQKLPEPIFTPAAKAEMGEHDENISFDEVEARIGKELAAKMRDISIRLYKEAADYAATRGIIIADTKFEFGLDENGTLTLMDEVLTADSSRFWPADSYQVGTNPPSFDKQFVRDWLEAVRIDGKPWPKTAPAPKLPEDVVQKTADKYKEALTRLTGEALQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-10-07" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LR79</accession>
    <name>Q1LR79_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_0461</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07347.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF07347">
        <property type="protein sequence ID" value="ABF07347"/>
        <property type="gene ID" value="Rmet_0461"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0461"/>
    <dbReference type="HOGENOM" id="CLU_2525140_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF07347.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="84" mass="9263" checksum="6D3787CF337990DB" modified="2006-05-30" version="1">MFRIVALGNLRARAMPRGSPLTSGSQAKQVATRITSKAAAHPPQATAELPWRRYQNEAKRFWLDARPQTVQVARQGCATTPESC</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="98" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LRF5</accession>
    <name>Q1LRF5_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Acyl-CoA dehydrogenase</fullName>
            <ecNumber evidence="10">1.3.8.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">Rmet_0385</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the acyl-CoA dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.3.8.-" evidence="10"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07271.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515263.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0385"/>
    <dbReference type="EnsemblBacteria" id="ABF07271">
        <property type="protein sequence ID" value="ABF07271"/>
        <property type="gene ID" value="Rmet_0385"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0385"/>
    <dbReference type="eggNOG" id="COG1960">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018204_12_2_4"/>
    <dbReference type="OMA" id="MWAKMAK"/>
    <dbReference type="OrthoDB" id="357522at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016627">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-CH group of donors"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.540.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.110.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025878">
        <property type="entry name" value="Acyl-CoA_dh-like_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020953">
        <property type="entry name" value="Acyl-CoA_DH_N_bac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006091">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046373">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036250">
        <property type="entry name" value="AcylCo_DH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009075">
        <property type="entry name" value="AcylCo_DH/oxidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013786">
        <property type="entry name" value="AcylCoA_DH/ox_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037069">
        <property type="entry name" value="AcylCoA_DH/ox_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009100">
        <property type="entry name" value="AcylCoA_DH/oxidase_NM_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00441">
        <property type="entry name" value="Acyl-CoA_dh_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12806">
        <property type="entry name" value="Acyl-CoA_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02770">
        <property type="entry name" value="Acyl-CoA_dh_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02771">
        <property type="entry name" value="Acyl-CoA_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12418">
        <property type="entry name" value="AcylCoA_DH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47203">
        <property type="entry name" value="SSF47203"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56645">
        <property type="entry name" value="SSF56645"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0274">FAD</keyword>
    <keyword evidence="3" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="10" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AcylCoA_DH_N" evidence="8">
        <location>
            <begin position="3"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA_dh_N" evidence="7">
        <location>
            <begin position="41"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA_dh_M" evidence="6">
        <location>
            <begin position="164"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA_dh_1" evidence="5">
        <location>
            <begin position="283"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA_dh_C" evidence="9">
        <location>
            <begin position="471"/>
            <end position="591"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF02770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF02771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF12418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF12806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ABF07271.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="595" mass="64585" checksum="93EFF319DF807E82" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="63" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LRG3</accession>
    <name>Q1LRG3_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_0377</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07263.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515255.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0377"/>
    <dbReference type="EnsemblBacteria" id="ABF07263">
        <property type="protein sequence ID" value="ABF07263"/>
        <property type="gene ID" value="Rmet_0377"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0377"/>
    <dbReference type="eggNOG" id="COG2928">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_068050_1_1_4"/>
    <dbReference type="OMA" id="PTTGWYT"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007462">
        <property type="entry name" value="COV1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31876">
        <property type="entry name" value="PTHR31876"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04367">
        <property type="entry name" value="DUF502"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="54"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="205"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="219"/>
            <end position="235"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF07263.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="235" mass="25404" checksum="B1B6246795AEDBF8" modified="2006-05-30" version="1">MVTKKTSALKTWFLTGLLVLVPLGITLWVLNAVISTMDQSMALLPLAWQPENLFGLRIPGLGAILTLLFILVVGVLAHNFIGQRLVKWWEALLRHIPVVGPIYTSVKQVSDTLLSSSGNAFRKALLVQYPREGSWTIAFLTGRPGGDVQNHLQGDYVSVYVPTTPNPTSGFFLMMPRADTIELDMTVDAALKYIVSMGVVAPADLPRKSGADTRPAHGAVADADADDAVADTHTN</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LRJ2</accession>
    <name>Q1LRJ2_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">ABC-type multidrug transporter, permease component</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Rmet_0348</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07234.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515227.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0348"/>
    <dbReference type="DNASU" id="4037135"/>
    <dbReference type="EnsemblBacteria" id="ABF07234">
        <property type="protein sequence ID" value="ABF07234"/>
        <property type="gene ID" value="Rmet_0348"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0348"/>
    <dbReference type="eggNOG" id="COG0842">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039483_8_0_4"/>
    <dbReference type="OMA" id="WVPVFAM"/>
    <dbReference type="OrthoDB" id="1552415at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043190">
        <property type="term" value="C:ATP-binding cassette (ABC) transporter complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000412">
        <property type="entry name" value="ABC_2_transport"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51012">
        <property type="entry name" value="ABC_TM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4 5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="32"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="191"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="239"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="273"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="305"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="361"/>
            <end position="379"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00442"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABF07234.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="384" mass="42602" checksum="3864594D42F647C0" modified="2006-05-30" version="1">MKAVRRQISRFSAHRWWSIVLKEFLQLRRDRVTFGMMIGIPIIQLLLFGFAINSDPRHMPTAVIVAEHSDFSRTFIAALDNTTYFKVVRTMPDEASGREALMRGDVQFVVSIPADFSRRLLHGDRPSILVEADATDPSATGQAIAALAQLPATVARIDLKGALAPLAGGQPAFDVNVHRLYNPEGITQYNIVPGLMGTILTMTMVMMTGLAMTRERERGTMENLLATPVQPLEVISGKIIPYIFIGLIQATIILLAAHFVFGVPFVGSPVAVYLAALLFIAANLTVGITLSSLAANQLQALQLTFFYFLPNILLSGFMFPFAGMPTWAQWIGNVLPMTYFNRMIRSIMLKGSGWIELWPNVWPVALFTVIVLTIAVRFYKRTLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LRS3</accession>
    <name>Q1LRS3_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">SURF1-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Rmet_0267</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cell membrane</location>
            <topology evidence="4">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the SURF1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07153.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0267"/>
    <dbReference type="EnsemblBacteria" id="ABF07153">
        <property type="protein sequence ID" value="ABF07153"/>
        <property type="gene ID" value="Rmet_0267"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0267"/>
    <dbReference type="eggNOG" id="COG3346">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047737_2_0_4"/>
    <dbReference type="OMA" id="NRGWLPW"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd06662">
        <property type="entry name" value="SURF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002994">
        <property type="entry name" value="Surf1/Shy1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045214">
        <property type="entry name" value="Surf1/Surf4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23427">
        <property type="entry name" value="PTHR23427"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02104">
        <property type="entry name" value="SURF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50895">
        <property type="entry name" value="SURF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="3 4" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2 4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="32"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="242"/>
            <end position="261"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU363076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABF07153.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="272" mass="29423" checksum="C6B61B5EF660CCED" modified="2006-05-30" version="1">MGARSAKRPLSHCDARREPVTTRTLWRSPGTVPMVAALVVIVVTCALGNWQLRRAHEKIDRAERLATLAKQAPVELRAGQTDAAQLVERRVRARGTFDADKTVLLDNRPHGNGTDSRAGFLVLTPLRLTDGGSVLVMRGWLPRDAQDRTRIAPFPTPTGEVTVEGTALAAVPRVYSLGQDASAEAGRKIRQNIDLAAFSHEIGASLLPVVVEQSGDAGDGLARDWAPADFGADRHYGYATQWFGLAVLTLVLVAVLGWRRARLDRADGNGNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LRU6</accession>
    <name>Q1LRU6_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Phosphotransferase system, fructose-specific component IIA</fullName>
            <ecNumber evidence="4">2.7.1.69</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Rmet_0244</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.7.1.69" evidence="4"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07130.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008645870.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0244"/>
    <dbReference type="EnsemblBacteria" id="ABF07130">
        <property type="protein sequence ID" value="ABF07130"/>
        <property type="gene ID" value="Rmet_0244"/>
    </dbReference>
    <dbReference type="GeneID" id="60822969"/>
    <dbReference type="KEGG" id="rme:Rmet_0244"/>
    <dbReference type="eggNOG" id="COG2893">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_123235_0_0_4"/>
    <dbReference type="OMA" id="VRAICYR"/>
    <dbReference type="OrthoDB" id="2008912at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0103111">
        <property type="term" value="F:D-glucosamine PTS permease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008982">
        <property type="term" value="F:protein-N(PI)-phosphohistidine-sugar phosphotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009401">
        <property type="term" value="P:phosphoenolpyruvate-dependent sugar phosphotransferase system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00006">
        <property type="entry name" value="PTS_IIA_man"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.510">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004701">
        <property type="entry name" value="PTS_EIIA_man-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036662">
        <property type="entry name" value="PTS_EIIA_man-typ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033887">
        <property type="entry name" value="PTS_IIA_man"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03610">
        <property type="entry name" value="EIIA-man"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53062">
        <property type="entry name" value="SSF53062"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51096">
        <property type="entry name" value="PTS_EIIA_TYPE_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PTS EIIA type-4" evidence="3">
        <location>
            <begin position="1"/>
            <end position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51096"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF07130.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="151" mass="15797" checksum="DA8EF8D03D60141C" modified="2006-05-30" version="1">MAGILIIAHAPLASALRDCAGHVYCGQPQRVEAIDVIADADPAAVLAEARKRLADVCEENGALVLTDIFGATPANIAARLAEPGRVKVLAGVNLPMLVRAICYRTEKLDQLATKALAGGAQGVLQIGTTAVQNQTANHPDKYAAEGHHHHQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="63" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LRY1</accession>
    <name>Q1LRY1_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_0209</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07095.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515114.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0209"/>
    <dbReference type="EnsemblBacteria" id="ABF07095">
        <property type="protein sequence ID" value="ABF07095"/>
        <property type="gene ID" value="Rmet_0209"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0209"/>
    <dbReference type="HOGENOM" id="CLU_149882_0_0_4"/>
    <dbReference type="OMA" id="AFGECPR"/>
    <dbReference type="OrthoDB" id="1720932at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021317">
        <property type="entry name" value="DUF2917"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011051">
        <property type="entry name" value="RmlC_Cupin_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11142">
        <property type="entry name" value="DUF2917"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51182">
        <property type="entry name" value="SSF51182"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF07095.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="112" mass="12820" checksum="A424FC1046E92D26" modified="2006-05-30" version="1">MRELRTFELEEPDQPVTWRARHGHSVTALEGRLWLTVEGHLADIWLRPGDVMTLPEGTRVWLSGDAPGSRFLLTESPAPWSLRRAVFITRQFARRWGEHKTAAFGDCPRISA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="104" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LRY9</accession>
    <name>Q1LRY9_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">3-isopropylmalate dehydratase small subunit</fullName>
            <ecNumber evidence="10">4.2.1.33</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Alpha-IPM isomerase</fullName>
            <shortName evidence="10">IPMI</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">Isopropylmalate isomerase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10 12" type="primary">leuD</name>
        <name evidence="12" type="ordered locus">Rmet_0201</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2 10">Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 10">
            <text>(2R,3S)-3-isopropylmalate = (2S)-2-isopropylmalate</text>
            <dbReference type="Rhea" id="RHEA:32287"/>
            <dbReference type="ChEBI" id="CHEBI:1178"/>
            <dbReference type="ChEBI" id="CHEBI:35121"/>
            <dbReference type="EC" id="4.2.1.33"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3 10">Amino-acid biosynthesis; L-leucine biosynthesis; L-leucine from 3-methyl-2-oxobutanoate: step 2/4.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5 10">Heterodimer of LeuC and LeuD.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 10">Belongs to the LeuD family. LeuD type 1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.33" evidence="10"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07087.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515107.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0201"/>
    <dbReference type="EnsemblBacteria" id="ABF07087">
        <property type="protein sequence ID" value="ABF07087"/>
        <property type="gene ID" value="Rmet_0201"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0201"/>
    <dbReference type="eggNOG" id="COG0066">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_081378_0_3_4"/>
    <dbReference type="OMA" id="NCQRNGV"/>
    <dbReference type="OrthoDB" id="1384217at2"/>
    <dbReference type="UniPathway" id="UPA00048">
        <property type="reaction ID" value="UER00071"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009316">
        <property type="term" value="C:3-isopropylmalate dehydratase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003861">
        <property type="term" value="F:3-isopropylmalate dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016853">
        <property type="term" value="F:isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009098">
        <property type="term" value="P:leucine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01577">
        <property type="entry name" value="IPMI_Swivel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.19.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01031">
        <property type="entry name" value="LeuD_type1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004431">
        <property type="entry name" value="3-IsopropMal_deHydase_ssu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015928">
        <property type="entry name" value="Aconitase/3IPM_dehydase_swvl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000573">
        <property type="entry name" value="AconitaseA/IPMdHydase_ssu_swvl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033940">
        <property type="entry name" value="IPMI_Swivel"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00694">
        <property type="entry name" value="Aconitase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00171">
        <property type="entry name" value="leuD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="9 10" id="KW-0100">Branched-chain amino acid biosynthesis</keyword>
    <keyword evidence="12" id="KW-0413">Isomerase</keyword>
    <keyword evidence="6 10" id="KW-0432">Leucine biosynthesis</keyword>
    <keyword evidence="8 10 12" id="KW-0456">Lyase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aconitase_C" evidence="11">
        <location>
            <begin position="3"/>
            <end position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ABF07087.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="217" mass="23524" checksum="7092EF1974CC107B" modified="2006-05-30" version="1">MDDITTIEGVGAPLPLPNLDTDQIMPKQFLRGIDKAGLDRGLLHDMRFDANGAPRADFVLNQPAWRDTRVLVAGPNFGCGSSREHAVWGLQQYGIRAVIAPSFGEIFYSNAMNNRLLLVALPQADVDQIMAAVSDPVAPASIRIDLSTMTVNAGDFAKSFSLSPRHLDMFRLGLDVIGMSLTHADAIAAFTSRHHADNPWLRDVAATTMARLGRMAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="55" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LS22</accession>
    <name>Q1LS22_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_0168</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07054.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515078.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF07054">
        <property type="protein sequence ID" value="ABF07054"/>
        <property type="gene ID" value="Rmet_0168"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0168"/>
    <dbReference type="eggNOG" id="ENOG5033XRI">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_119537_0_0_4"/>
    <dbReference type="OMA" id="RRMDEPF"/>
    <dbReference type="OrthoDB" id="1430044at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025688">
        <property type="entry name" value="PGDYG_prot"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14083">
        <property type="entry name" value="PGDYG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF07054.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="147" mass="16167" checksum="0E15D95BF31B21DA" modified="2006-05-30" version="1">MQTLTNIDLREDRAANWYVKDETVQVAFATEPGELISREGPNRYQPGDAIVTGATGDRWVVSRGRFDPRYVAVGPNAHGESGPYRNKPVPVLAKEMTHAFAIARSTGGDVLTGKPGDWLMQYAPGDYGITERARFEAVYRPADGPKD</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-01-15" modified="2022-05-25" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LSE1</accession>
    <name>GATA_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Glutamyl-tRNA(Gln) amidotransferase subunit A</fullName>
            <shortName evidence="1">Glu-ADT subunit A</shortName>
            <ecNumber evidence="1">6.3.5.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">gatA</name>
        <name type="ordered locus">Rmet_0049</name>
    </gene>
    <organism>
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Allows the formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu-tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activated gamma-phospho-Glu-tRNA(Gln).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + H2O + L-glutamine + L-glutamyl-tRNA(Gln) = ADP + H(+) + L-glutamate + L-glutaminyl-tRNA(Gln) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:17521"/>
            <dbReference type="Rhea" id="RHEA-COMP:9681"/>
            <dbReference type="Rhea" id="RHEA-COMP:9684"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:78520"/>
            <dbReference type="ChEBI" id="CHEBI:78521"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.5.7"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Heterotrimer of A, B and C subunits.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the amidase family. GatA subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.3.5.7" evidence="1"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF06935.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011514976.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q1LSE1"/>
    <dbReference type="SMR" id="Q1LSE1"/>
    <dbReference type="STRING" id="266264.Rmet_0049"/>
    <dbReference type="EnsemblBacteria" id="ABF06935">
        <property type="protein sequence ID" value="ABF06935"/>
        <property type="gene ID" value="Rmet_0049"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0049"/>
    <dbReference type="eggNOG" id="COG0154">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009600_0_3_4"/>
    <dbReference type="OMA" id="EVSCPHF"/>
    <dbReference type="OrthoDB" id="1239251at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030956">
        <property type="term" value="C:glutamyl-tRNA(Gln) amidotransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050567">
        <property type="term" value="F:glutaminyl-tRNA synthase (glutamine-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1300.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00120">
        <property type="entry name" value="GatA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000120">
        <property type="entry name" value="Amidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020556">
        <property type="entry name" value="Amidase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023631">
        <property type="entry name" value="Amidase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036928">
        <property type="entry name" value="AS_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004412">
        <property type="entry name" value="GatA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006594">
        <property type="entry name" value="LisH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11895">
        <property type="entry name" value="PTHR11895"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01425">
        <property type="entry name" value="Amidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75304">
        <property type="entry name" value="SSF75304"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00132">
        <property type="entry name" value="gatA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00571">
        <property type="entry name" value="AMIDASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_1000015891" description="Glutamyl-tRNA(Gln) amidotransferase subunit A">
        <location>
            <begin position="1"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="1">
        <location>
            <position position="80"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="1">
        <location>
            <position position="155"/>
        </location>
    </feature>
    <feature type="active site" description="Acyl-ester intermediate" evidence="1">
        <location>
            <position position="179"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00120"/>
        </source>
    </evidence>
    <sequence length="499" mass="53022" checksum="C4EF36A1822CF91C" modified="2006-05-30" version="1">MPFSADSVTSLRQIADALAARSVSAEELAREYLARIEAGRALNAFVAVDPELTLAQARAADERRAQGQATPLTGVPIAHKDVFVTRGWKSTAGSKMLANYESPFDATVVERLAAAGMVTLGKTNMDEFAMGSSNENSFFGPVSNPWDTSRVPGGSSGGSAAAVAAGLAPAATGTDTGGSIRQPASFSGITGIKPTYGRVSRYGMIAFASSLDQGGPMAHSAEDCALLLNGMAGFDPKDSTSLTPELGGVTEDFTRLLGQPRAGATASQPLAGLRIGLPREYFGKGLSADVEQALRAALAEYEKLGATLVDVTLPKTELSIPVYYIIAPAEASSNLSRFDGVRYGHRAAQYGDLLDMYKKSRAEGFGPEVKRRIMVGTYVLSHGYYDAYYLQAQKIRRIIADDFQRAFTQCDVIMGPVAPTVAWKLGEKTADPVQMYLADIFTLSTSLAGLPGMSVPCGFGEGNMPVGLQLIGNYFDEAQLLQTAHAFQQATDWHLRRPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-04-26" modified="2021-09-29" version="67" xmlns="http://uniprot.org/uniprot">
    <accession>Q58AG0</accession>
    <name>Q58AG0_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Protein involved in metal resistance</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">mmrQ</name>
        <name evidence="2" type="ordered locus">Rmet_6143</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2 3">
        <name>pMOL30</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid pMOL30</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000354">
        <property type="protein sequence ID" value="ABF13002.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008642700.1">
        <property type="nucleotide sequence ID" value="NC_007971.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_145658.1">
        <property type="nucleotide sequence ID" value="NC_006466.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF13002">
        <property type="protein sequence ID" value="ABF13002"/>
        <property type="gene ID" value="Rmet_6143"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6143"/>
    <dbReference type="HOGENOM" id="CLU_136209_5_1_4"/>
    <dbReference type="OrthoDB" id="2229280at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid pMOL30"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5013531916" evidence="1">
        <location>
            <begin position="23"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF13002.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="81" mass="8273" checksum="64829551FF788654" modified="2005-04-26" version="1">MKKLHTAILTAALSILAGTAVAAPAEGQVHRPRDPFTDGARAGASASDVYAGGARIDKRDVFTDGARIGARDVFTDGAHGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-01-04" modified="2022-05-25" version="68" xmlns="http://uniprot.org/uniprot">
    <accession>Q5NUV8</accession>
    <name>Q5NUV8_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_6230</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="1 2">
        <name>pMOL28</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid pMOL28</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000355">
        <property type="protein sequence ID" value="ABF13089.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011239952.1">
        <property type="nucleotide sequence ID" value="NC_007972.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_161691.1">
        <property type="nucleotide sequence ID" value="NC_006525.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF13089">
        <property type="protein sequence ID" value="ABF13089"/>
        <property type="gene ID" value="Rmet_6230"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6230"/>
    <dbReference type="HOGENOM" id="CLU_162029_0_0_4"/>
    <dbReference type="OMA" id="ICADDYL"/>
    <dbReference type="OrthoDB" id="2078365at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid pMOL28"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF13089.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="130" mass="14050" checksum="A82DDCC6EC93A1F4" modified="2005-01-04" version="1">MSRTVYGAGLASYPAGVLLRASVYSTNLRPAFSLVCADDQMPYGVLSVNLPDANLADDEFLVTGDWNLPTDLKAALLETGKFVQTGRWNQVGFDQGEVWRIVDAEILTEVAAARVVASRSRSDRRTAAVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-01-04" modified="2022-05-25" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>Q5NUZ0</accession>
    <name>Q5NUZ0_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="9">TnpA Transposase Tn4378 mercury transposon Tn3 family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">tnpA</name>
        <name evidence="9" type="ordered locus">Rmet_6188</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="9 10">
        <name>pMOL28</name>
    </geneLocation>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="10">Plasmid pMOL28</plasmid>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the transposase 7 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000355">
        <property type="protein sequence ID" value="ABF13047.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011239983.1">
        <property type="nucleotide sequence ID" value="NC_007972.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_161722.1">
        <property type="nucleotide sequence ID" value="NC_006525.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF13047">
        <property type="protein sequence ID" value="ABF13047"/>
        <property type="gene ID" value="Rmet_6188"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6188"/>
    <dbReference type="HOGENOM" id="CLU_009098_1_0_4"/>
    <dbReference type="OMA" id="YPHAMRT"/>
    <dbReference type="OrthoDB" id="45599at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid pMOL28"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004803">
        <property type="term" value="F:transposase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006313">
        <property type="term" value="P:transposition, DNA-mediated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025296">
        <property type="entry name" value="DUF4158"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002513">
        <property type="entry name" value="Tn3_Tnp_DDE_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01526">
        <property type="entry name" value="DDE_Tnp_Tn3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13700">
        <property type="entry name" value="DUF4158"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
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    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0814">Transposable element</keyword>
    <keyword evidence="3" id="KW-0815">Transposition</keyword>
    <feature type="domain" description="DUF4158" evidence="8">
        <location>
            <begin position="6"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="domain" description="DDE_Tnp_Tn3" evidence="7">
        <location>
            <begin position="580"/>
            <end position="967"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="672"/>
            <end position="697"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009402"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022578"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01526"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF13700"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ABF13047.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="989" mass="111500" checksum="C179430F3E1E66CA" modified="2005-01-04" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2010-03-23" modified="2020-10-07" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>D3DXM7</accession>
    <name>D3DXM7_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name evidence="1" type="ordered locus">Rmet_6430</name>
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    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
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            <taxon>Betaproteobacteria</taxon>
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        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ADC45047.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADC45047">
        <property type="protein sequence ID" value="ADC45047"/>
        <property type="gene ID" value="Rmet_6430"/>
    </dbReference>
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    <dbReference type="HOGENOM" id="CLU_1702808_0_0_4"/>
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        <property type="component" value="Chromosome"/>
    </dbReference>
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    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADC45047.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="154" mass="16077" checksum="C2C90E24469C939B" modified="2010-03-23" version="1">MDASAEEAGSGAPEPTVVAEADCPLVAGRPCGARDAGSAGGATAGSGGGKVMGTAPVIQRNARLKAHICEATMNHRPSRQSGRYLLQHLDANLARCDLAERHHGGLVLCLDLGRVALHELTCTVRRGEGELEAVRDVFQTIFDGNTRHIKSTLG</sequence>
</entry>
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    <accession>D3DXT7</accession>
    <name>D3DXT7_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_6499</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
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        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ADC45107.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_6499"/>
    <dbReference type="EnsemblBacteria" id="ADC45107">
        <property type="protein sequence ID" value="ADC45107"/>
        <property type="gene ID" value="Rmet_6499"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6499"/>
    <dbReference type="HOGENOM" id="CLU_2809360_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADC45107.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="67" mass="7667" checksum="D8B12D1B1545BEE0" modified="2010-03-23" version="1">MRWGDPVSSIPNARKTQSLAIGEHCYERPDDTLTMGKATLSDIRYCFYKNWSGPLNLRTPGTSYNKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-03-23" modified="2020-10-07" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>D3DY70</accession>
    <name>D3DY70_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_6640</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ADC45240.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_6640"/>
    <dbReference type="EnsemblBacteria" id="ADC45240">
        <property type="protein sequence ID" value="ADC45240"/>
        <property type="gene ID" value="Rmet_6640"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6640"/>
    <dbReference type="HOGENOM" id="CLU_3029134_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="39"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADC45240.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="55" mass="5996" checksum="418B9A5C3D04DBCD" modified="2010-03-23" version="1">MSMSDWTSSVLVAECGAWDMIVSVSDLSATALAFLTGVWRGRFAFIVLRANHCSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-03-23" modified="2020-10-07" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>D3DYG7</accession>
    <name>D3DYG7_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_6758</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ADC45337.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_6758"/>
    <dbReference type="EnsemblBacteria" id="ADC45337">
        <property type="protein sequence ID" value="ADC45337"/>
        <property type="gene ID" value="Rmet_6758"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6758"/>
    <dbReference type="HOGENOM" id="CLU_2938425_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADC45337.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="60" mass="6950" checksum="7FF8912DD5E14769" modified="2010-03-23" version="1">MLLEMSCLDYLVDPDSKPIRKILLLRRYNAGCPLSMNDHAQAHRVGHSWLSAHHVHCRSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="51" xmlns="http://uniprot.org/uniprot">
    <accession>Q1L9J2</accession>
    <name>Q1L9J2_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_6325</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="1 2">
        <name>pMOL28</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid pMOL28</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000355">
        <property type="protein sequence ID" value="ABF13184.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011514919.1">
        <property type="nucleotide sequence ID" value="NC_007972.2"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF13184">
        <property type="protein sequence ID" value="ABF13184"/>
        <property type="gene ID" value="Rmet_6325"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6325"/>
    <dbReference type="HOGENOM" id="CLU_038463_0_0_4"/>
    <dbReference type="OMA" id="NLSEARW"/>
    <dbReference type="OrthoDB" id="1036927at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid pMOL28"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025359">
        <property type="entry name" value="DUF4263"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14082">
        <property type="entry name" value="DUF4263"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF13184.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="479" mass="54245" checksum="440AB61CB44641A7" modified="2006-05-30" version="1">MEQGRLEVTATFNNNEVLNPSFELSHGVDQGVQYRDLYLQTYQRGANDPNVWVAQPREQWHCLARVYPDRLLTYPVFTNPHAQRYLSPKHGPLTSVIFHRKVDEPLPETADAAVTEIDLSLPWKCFSESTYGLGLVPELDEVWRSLSGIPAITAIGVTRSGTSTVTESIAWITVSDLHRFRRNFQRFKRLAREHVHVARRWDVRNNLLATLDPDRFPHMAPAAAAAPLVEYVRTRGRRADVSERAQRQAAVRAVRQELATIATEAPAEVLQLHAEIERVTLARMIDRYATMLEQSLPEPRWQQFFENNLFVLSLVFSRPVRLLHTQFHAQASRLDGTGAQIGDFLFAEQGQALAIVEIKKPSSSLVQATPYRNREVFGPHAELSGAITQVLYQRSAIQSNWLLHRAEAALRDSQPDAIKCIVLAGTMPSDETQRRSFELFRNACKDVEVVTFDELLGKLRLLLQHLTPSSGTTEDTVPF</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-10-07" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LA89</accession>
    <name>Q1LA89_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_6078</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2 3">
        <name>pMOL30</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid pMOL30</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000354">
        <property type="protein sequence ID" value="ABF12937.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF12937">
        <property type="protein sequence ID" value="ABF12937"/>
        <property type="gene ID" value="Rmet_6078"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6078"/>
    <dbReference type="HOGENOM" id="CLU_1748149_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid pMOL30"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="76"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF12937.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="149" mass="16661" checksum="A98128D2CAE64C52" modified="2006-05-30" version="1">MSTRRIDHPLNLNWRQLRAIVSFLELIMTTDTGGSSSEPVALADRAEVNHVKRHIRKCQRHLARLDGHLTSAFDKALDNSDDDTDPEELQTLGEQKTQCEQAARKYSEAIGLASAKKLTEEQFAVVTTMVNEADVALNEAEPMGWPSMK</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-10-07" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LAE0</accession>
    <name>Q1LAE0_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_6027</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="1 2">
        <name>pMOL30</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid pMOL30</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000354">
        <property type="protein sequence ID" value="ABF12886.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF12886">
        <property type="protein sequence ID" value="ABF12886"/>
        <property type="gene ID" value="Rmet_6027"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6027"/>
    <dbReference type="HOGENOM" id="CLU_2411062_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid pMOL30"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF12886.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="92" mass="10323" checksum="C61B8A7581533E7E" modified="2006-05-30" version="1">MTEDFSSFLATDARTKRRLPVDLQRLWRLNRGLCPVHGQPLSLSIQAEPIDRARDLSPLECPVAGCSAKVSAWDWNLPGNENRPYCIESLGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LAP0</accession>
    <name>Q1LAP0_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Aldo/keto oxidoreductase, NADP-binding</fullName>
            <ecNumber evidence="2">1.1.1.91</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">mocA</name>
        <name evidence="2" type="ordered locus">Rmet_5927</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EC" id="1.1.1.91" evidence="2"/>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12786.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518133.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5927"/>
    <dbReference type="EnsemblBacteria" id="ABF12786">
        <property type="protein sequence ID" value="ABF12786"/>
        <property type="gene ID" value="Rmet_5927"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5927"/>
    <dbReference type="eggNOG" id="COG0667">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023205_2_0_4"/>
    <dbReference type="OMA" id="VDLWQVH"/>
    <dbReference type="OrthoDB" id="1351747at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047681">
        <property type="term" value="F:aryl-alcohol dehydrogenase (NADP+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023210">
        <property type="entry name" value="NADP_OxRdtase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036812">
        <property type="entry name" value="NADP_OxRdtase_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00248">
        <property type="entry name" value="Aldo_ket_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51430">
        <property type="entry name" value="SSF51430"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aldo_ket_red" evidence="1">
        <location>
            <begin position="21"/>
            <end position="314"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF12786.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="341" mass="37029" checksum="4C8B84ECA5495DF4" modified="2006-05-30" version="1">MPDFAMQYRRLGASNLNVSTLCLGTMMFADQTDEAEAARIVASAREHGVNFIDTADVYSKGASEQMVGRLLAGSRHDWVLATKLGNAMGSGPNQSNYSRTWIMRSVEDSLTRLATDYIDILYLHRDFAGANLEEPVRAMGDLIRAGKIRYWAVSNFRGWRIAEIAALCDRLGVPRPVACQPYYNLLNRVPEVEILPACQHFGLGVVPYSPIARGVLTGKYLPGQAPGAETRAGRADRRIMETEFREESLVIAQTLKSHAEGRGLTPGQFATAWVLANPIISSVIAGPRTLAQFEDYFGAVAAAITPAEEAMVDGLVPRGHASTHGYNDPGYPFGGRPVAAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="110" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LAQ5</accession>
    <name>Q1LAQ5_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Transcriptional regulator Ada / DNA-3-methyladenine glycosylase II / DNA-O6-methylguanine--protein-cysteine S-methyltransferase</fullName>
            <ecNumber evidence="6">2.1.1.63</ecNumber>
            <ecNumber evidence="6">3.2.2.21</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Rmet_5912</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="7">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="7">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <dbReference type="EC" id="2.1.1.63" evidence="6"/>
    <dbReference type="EC" id="3.2.2.21" evidence="6"/>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12771.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518121.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5912"/>
    <dbReference type="EnsemblBacteria" id="ABF12771">
        <property type="protein sequence ID" value="ABF12771"/>
        <property type="gene ID" value="Rmet_5912"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5912"/>
    <dbReference type="eggNOG" id="COG0122">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG2169">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_72_6_4"/>
    <dbReference type="OMA" id="RWPDAFP"/>
    <dbReference type="OrthoDB" id="1781735at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008725">
        <property type="term" value="F:DNA-3-methyladenine glycosylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052822">
        <property type="term" value="F:DNA-3-methylguanine glycosylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052821">
        <property type="term" value="F:DNA-7-methyladenine glycosylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043916">
        <property type="term" value="F:DNA-7-methylguanine glycosylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003908">
        <property type="term" value="F:methylated-DNA-[protein]-cysteine S-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006284">
        <property type="term" value="P:base-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00056">
        <property type="entry name" value="ENDO3c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1670.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.310.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035451">
        <property type="entry name" value="Ada-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004026">
        <property type="entry name" value="Ada_DNA_repair_Zn-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010316">
        <property type="entry name" value="AlkA_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037046">
        <property type="entry name" value="AlkA_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011257">
        <property type="entry name" value="DNA_glycosylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003265">
        <property type="entry name" value="HhH-GPD_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023170">
        <property type="entry name" value="HhH_base_excis_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018060">
        <property type="entry name" value="HTH_AraC"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02805">
        <property type="entry name" value="Ada_Zn_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06029">
        <property type="entry name" value="AlkA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12833">
        <property type="entry name" value="HTH_18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01009">
        <property type="entry name" value="AlkA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00478">
        <property type="entry name" value="ENDO3c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00342">
        <property type="entry name" value="HTH_ARAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48150">
        <property type="entry name" value="SSF48150"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57884">
        <property type="entry name" value="SSF57884"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01124">
        <property type="entry name" value="HTH_ARAC_FAMILY_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0227">DNA damage</keyword>
    <keyword evidence="4" id="KW-0234">DNA repair</keyword>
    <keyword evidence="6" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 6" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="6" id="KW-0614">Plasmid</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="HTH araC/xylS-type" evidence="5">
        <location>
            <begin position="87"/>
            <end position="185"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS01124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABF12771.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="500" mass="53811" checksum="BBE401AFDD9170C2" modified="2006-05-30" version="1">MELDPDTCYKAVASHDRRFDGRFFVGVSSTGVYCRPICAVRTPKRENCTFYETAAAAEKHGFRPCLRCRPELAPGHGLADISGRLAQAAATLIDEGFMAGAGVAQLAARIGVTERHLRRLFDAEFGVSVHEYAQTQKLLLAKRLLTDTALPVTEVALAAGFGSVRRFNDVLKERYGLTPLAMRKRAADGMADRLVFELGYRPPMAWGALLGFLAVRAVDGIEQCDGEVYSRTLSIEAGGRCHAGCVRLEHVARRAVLRITLSGALAPVIPQALGKVRRLCDLGCRPDIVDAHLGELAARTPGMRLPGAFDGFEIGVRAIVGQVIPVVQARRILARLAQVAGEPLPEAAQAGLTTPAGQAPLTHAFPTAQALAALPDEAYRAAGVTAAKTRTIRALARSVAAHEIDLDPHAPPEVTVAQLRAIDGIGDWTAQYVAMRALGWPDAFPGTDYALRKVLGVDTVRGMQAKTAGWAPWRAYAAIHLWHRYEALKLQGSDIEEQAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="61" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LAW0</accession>
    <name>Q1LAW0_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_5857</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12716.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518065.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5857"/>
    <dbReference type="EnsemblBacteria" id="ABF12716">
        <property type="protein sequence ID" value="ABF12716"/>
        <property type="gene ID" value="Rmet_5857"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5857"/>
    <dbReference type="HOGENOM" id="CLU_585087_0_0_4"/>
    <dbReference type="OrthoDB" id="2222339at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="42"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="78"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="111"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="155"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="187"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="218"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="342"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="375"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="408"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="431"/>
            <end position="450"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF12716.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="467" mass="50179" checksum="754913CB3B30ADA1" modified="2006-05-30" version="1">MTTIKGELLEGETETGMAAAAQGGQYGAAAERASEAKRALRLAVASSMVSKVCSIGLQAVSIPLFARVLDTDDFSAMMVYFGVTAWLSLVAIGIWPTVTSIAADDRRRGQLAGVLTVSWVGLSAIFASLIVLVLVLRPVLEQSGINLFSARHYSLFTAALVCYAMLTVLSVGEAVNQGQHRQHVNNLITAAGSILNVVFILLVTYFRPPGEEHVDVTLLFVASQAGFILIRVVNMIGVVVRAGWPHAFPGKAFARELARNSWSLLQAQLAVVFLQQGAVLLCYETDMPRDAALLALLFRANLLLHSFVYMINQPLWPIIHRNMLAGDWQWVIRIYRKLATCYVAYGLLAIIGAAFVGGAAVSLWTAGEYPISNKLATLCAVYFALLVTSAASTPVLMGAKAFVSLGNIGMAEFLAAGVLCTILYFADAVSFPHVVEALIAANAMTALWMMPTQAIVRLSRGSLDAQH</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="60" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LB36</accession>
    <name>Q1LB36_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_5781</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12640.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011520179.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5781"/>
    <dbReference type="EnsemblBacteria" id="ABF12640">
        <property type="protein sequence ID" value="ABF12640"/>
        <property type="gene ID" value="Rmet_5781"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5781"/>
    <dbReference type="eggNOG" id="ENOG5032YE4">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1406684_0_0_4"/>
    <dbReference type="OMA" id="YLYAWSE"/>
    <dbReference type="OrthoDB" id="1614808at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193279" evidence="1">
        <location>
            <begin position="20"/>
            <end position="215"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF12640.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="215" mass="23684" checksum="1EE8A0534EBFA9E4" modified="2006-05-30" version="1">MRHPVAALGIALAVSSAHAQEAAAPAANASQEAQPPAWNFSLAGYWNMPRQEQSYLSAIAIAERDKLHLEARANYEAMHAQSAFVGWSFAGGETFTYKVTPIIGFAMGSIHGVIPGLEASLAAYKFDYYIEAEYLTGSGTQTPSYLYAWSELGYRPVDWLRLGFVMQRTRIYGGDREFQRGGLVQLTFKNVTVGCYWFNPGSSEQVVMTNLTVSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="58" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LB65</accession>
    <name>Q1LB65_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_5752</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12611.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF12611">
        <property type="protein sequence ID" value="ABF12611"/>
        <property type="gene ID" value="Rmet_5752"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5752"/>
    <dbReference type="eggNOG" id="ENOG5033KGW">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_145403_0_0_4"/>
    <dbReference type="OMA" id="QTMENAK"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="128"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="12"/>
            <end position="47"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF12611.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="151" mass="16173" checksum="12C6921087A37141" modified="2006-05-30" version="1">MNPLSGVWPTKRAEGRGRRKQAVRKDSIMGEAKRRGTPEERAQQARARVDALRPAQLVCGACKTAFTNFDAIPAPGLHGIDAVFGGQCPNCGNDVVVFKGESKAVAEAMLAYERMMGESAKLGYQSADGRHVPFDADAPQASDASDESTKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="92" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LB86</accession>
    <name>Q1LB86_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Polysialic acid transport ATP-binding protein kpsT</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">kpsT</name>
        <name evidence="10" type="ordered locus">Rmet_5731</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="11">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="11">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ABC transporter superfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12590.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011520131.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5731"/>
    <dbReference type="EnsemblBacteria" id="ABF12590">
        <property type="protein sequence ID" value="ABF12590"/>
        <property type="gene ID" value="Rmet_5731"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5731"/>
    <dbReference type="eggNOG" id="COG1134">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_2_4"/>
    <dbReference type="OMA" id="VHIVYRV"/>
    <dbReference type="OrthoDB" id="887953at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd03220">
        <property type="entry name" value="ABC_KpsT_Wzt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015860">
        <property type="entry name" value="ABC_transpr_TagH-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-0614">Plasmid</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-1278">Translocase</keyword>
    <feature type="domain" description="ABC transporter" evidence="9">
        <location>
            <begin position="2"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="38"/>
            <end position="45"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ABF12590.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="221" mass="24803" checksum="6003110F33AB1E7C" modified="2006-05-30" version="1">MIELRQVWKRYSGHHSRNWVLRDINLCIRQGDRVGLIGRNGAGKSTLLNLIGGMDHPSRGQVRRDGKLSWTLGLNGGFQGALSGEQNARFVFRIHGVPDQEIAERTNFVHVFSELGDYFFAPMKTYSSGMRSRLAFALSLAFRFDTYLVDELIAVGDEKFKKKSKKAFEDLIASAGLIMVSHDEGTLKNFCEKGILMSNGAAYWFDDIADALSEYKKSINA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LBK6</accession>
    <name>Q1LBK6_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Cytochrome bo(3) ubiquinol oxidase subunit 4</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Cytochrome o ubiquinol oxidase subunit 4</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">Oxidase bo(3) subunit 4</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">Ubiquinol oxidase polypeptide IV</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">Ubiquinol oxidase subunit 4</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="primary">cyoD</name>
        <name evidence="17" type="ordered locus">Rmet_5611</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="18">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="18">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Cytochrome bo(3) ubiquinol terminal oxidase is the component of the aerobic respiratory chain of E.coli that predominates when cells are grown at high aeration. Has proton pump activity across the membrane in addition to electron transfer, pumping 2 protons/electron.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Heterooctamer of two A chains, two B chains, two C chains and two D chains.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the cytochrome c oxidase bacterial subunit 4 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12470.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008643179.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5611"/>
    <dbReference type="EnsemblBacteria" id="ABF12470">
        <property type="protein sequence ID" value="ABF12470"/>
        <property type="gene ID" value="Rmet_5611"/>
    </dbReference>
    <dbReference type="GeneID" id="60824426"/>
    <dbReference type="KEGG" id="rme:Rmet_5611"/>
    <dbReference type="eggNOG" id="COG3125">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_140945_0_0_4"/>
    <dbReference type="OMA" id="HYHMMIN"/>
    <dbReference type="OrthoDB" id="2088013at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009486">
        <property type="term" value="F:cytochrome bo3 ubiquinol oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015990">
        <property type="term" value="P:electron transport coupled proton transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005171">
        <property type="entry name" value="Cyt_c_oxidase_su4_prok"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014210">
        <property type="entry name" value="Cyt_o_ubiqinol_oxidase_su4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03626">
        <property type="entry name" value="COX4_pro"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02847">
        <property type="entry name" value="CyoD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="17" id="KW-0614">Plasmid</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 16" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="27"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="60"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="91"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008079"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011700"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00014689"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00025694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00030071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00030211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00031887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00032185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="ABF12470.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="142" mass="15574" checksum="A904563243EAA514" modified="2006-05-30" version="1">MSAQDHAMDGHGHGHEHEEEVGPHATLGGYLTGFVLSVFLTAVPFWLVMGKVFEKSSTTAIAILLIGAVQIVVHMIYFLHMNAKSEGGWNMLSLMFTLTLVVITLTGSLWVMFHLNSNMMPQMRHTQQAAESGGTIVPPRTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="52" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LBL9</accession>
    <name>Q1LBL9_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_5598</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12457.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008642633.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5598"/>
    <dbReference type="EnsemblBacteria" id="ABF12457">
        <property type="protein sequence ID" value="ABF12457"/>
        <property type="gene ID" value="Rmet_5598"/>
    </dbReference>
    <dbReference type="GeneID" id="60824413"/>
    <dbReference type="KEGG" id="rme:Rmet_5598"/>
    <dbReference type="HOGENOM" id="CLU_2272691_0_0_4"/>
    <dbReference type="OMA" id="IKADSQH"/>
    <dbReference type="OrthoDB" id="1747833at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF12457.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="103" mass="11177" checksum="389E21C21E9E665E" modified="2006-05-30" version="1">MQARFARGIAQTPLTVAAREFPLAEEAAMPEVGDLVTVFSQDLVTEFSIRLNAAAGPDLWYGVIYAISRGTEMLVVAEGLELDDVVSVRRQEVASVIKADSQH</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LBM0</accession>
    <name>Q1LBM0_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative hydrolase of the alpha/beta superfamily</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_5597</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12456.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5597"/>
    <dbReference type="EnsemblBacteria" id="ABF12456">
        <property type="protein sequence ID" value="ABF12456"/>
        <property type="gene ID" value="Rmet_5597"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5597"/>
    <dbReference type="eggNOG" id="COG2945">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_086287_1_0_4"/>
    <dbReference type="OMA" id="HFFKGCL"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022742">
        <property type="entry name" value="Hydrolase_4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12146">
        <property type="entry name" value="Hydrolase_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="SSF53474"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Hydrolase_4" evidence="1">
        <location>
            <begin position="72"/>
            <end position="159"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF12456.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="237" mass="24939" checksum="22FE31CA212531F6" modified="2006-05-30" version="1">MPETVTPAPSQQVAVPMSSPLPVPATTRQFIAGPAGQIEVLVDTPAAATIGVAVVAHPHPSQGGNAEHKIPQLLARILQAHGFLALRPNYRGVGQSEGEYDEGNGETDDVLAVIRYAQSANAGLPLALAGFSFGAFVQTRAAEVLTAEGESIAHLMLTGMPAGALSDTLSYDTPTVPAHALVVHGERDERVPLVNVFDWARPQELPVVVVPGAGHFFTGKLPGLRRVVESYLRRPAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="96" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LBV7</accession>
    <name>Q1LBV7_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Beta-ketoadipyl CoA thiolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Rmet_5510</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="7">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="7">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the thiolase-like superfamily. Thiolase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12369.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519912.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5510"/>
    <dbReference type="EnsemblBacteria" id="ABF12369">
        <property type="protein sequence ID" value="ABF12369"/>
        <property type="gene ID" value="Rmet_5510"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5510"/>
    <dbReference type="eggNOG" id="COG0183">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031026_2_3_4"/>
    <dbReference type="OMA" id="DIIMGCV"/>
    <dbReference type="OrthoDB" id="287662at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016747">
        <property type="term" value="F:acyltransferase activity, transferring groups other than amino-acyl groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00751">
        <property type="entry name" value="thiolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.47.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002155">
        <property type="entry name" value="Thiolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016039">
        <property type="entry name" value="Thiolase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020610">
        <property type="entry name" value="Thiolase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020617">
        <property type="entry name" value="Thiolase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020613">
        <property type="entry name" value="Thiolase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020616">
        <property type="entry name" value="Thiolase_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02803">
        <property type="entry name" value="Thiolase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00108">
        <property type="entry name" value="Thiolase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000429">
        <property type="entry name" value="Ac-CoA_Ac_transf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53901">
        <property type="entry name" value="SSF53901"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01930">
        <property type="entry name" value="AcCoA-C-Actrans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00737">
        <property type="entry name" value="THIOLASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00099">
        <property type="entry name" value="THIOLASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="6" id="KW-0614">Plasmid</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Thiolase_N" evidence="4">
        <location>
            <begin position="5"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="domain" description="Thiolase_C" evidence="5">
        <location>
            <begin position="280"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="active site" description="Acyl-thioester intermediate" evidence="2">
        <location>
            <position position="91"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="2">
        <location>
            <position position="358"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="2">
        <location>
            <position position="388"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR000429-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF02803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABF12369.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="402" mass="42632" checksum="31B9D07F94F906F7" modified="2006-05-30" version="1">MNEAYIFDALRTPRGRGRADGSLYEVNPVDLLAGLMTELQQRHDLDTAQVEDALIGCVMPNGEQSGCIAKTAALRANWDWQVPGVQLDRYCGSGLETVNIAAAKVRSGWENLLVAGGVESMSRITMGGAPGPRDTKPDMVFQLKPVPTGVAADLMATLDGHTREAVDRYALQSQQRAGHARASGYFDRSVVPVKDANGLTILKQDELIREDTTLEKLGALKASFQAMGELGGFDALAQMKYPEVEKVRHIHTGGNSSGIVDGASLLLVGSEQKGRELGLTPRARVVSVAAVGAEPTIMLAGPAPASRLALKKAGLSIDDIDLIEVNEAFASVVLRFMTEMQVDPAKVNVNGGAIAMGHPLGATGGMLLGTLLDELERRNLKRGLITLCVAGGMGIATIIERV</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-10-07" version="53" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LBZ2</accession>
    <name>Q1LBZ2_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_5475</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12334.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5475"/>
    <dbReference type="EnsemblBacteria" id="ABF12334">
        <property type="protein sequence ID" value="ABF12334"/>
        <property type="gene ID" value="Rmet_5475"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5475"/>
    <dbReference type="HOGENOM" id="CLU_180622_0_0_4"/>
    <dbReference type="OMA" id="SHYYWSA"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF12334.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="107" mass="11652" checksum="4E3B7102BE1A2EC9" modified="2006-05-30" version="1">MRNENGIMGNNRLAANCHLPGDSPAENPTPGANGILGRIPSQYVSAAVNLLPPAASTTASHQEIELAAGPVGKVRFFAEKKLARHHRHSHYFWSVYRAEPVRTDDLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LC30</accession>
    <name>Q1LC30_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Long-chain-acyl-CoA dehydrogenase</fullName>
            <ecNumber evidence="8">1.3.8.8</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Rmet_5437</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="9">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="9">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the acyl-CoA dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.3.8.8" evidence="8"/>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12296.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519843.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5437"/>
    <dbReference type="EnsemblBacteria" id="ABF12296">
        <property type="protein sequence ID" value="ABF12296"/>
        <property type="gene ID" value="Rmet_5437"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5437"/>
    <dbReference type="eggNOG" id="COG1960">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018204_0_3_4"/>
    <dbReference type="OMA" id="AGYMNEY"/>
    <dbReference type="OrthoDB" id="760677at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004466">
        <property type="term" value="F:long-chain-acyl-CoA dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.540.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.110.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006089">
        <property type="entry name" value="Acyl-CoA_DH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006091">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046373">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036250">
        <property type="entry name" value="AcylCo_DH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009075">
        <property type="entry name" value="AcylCo_DH/oxidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013786">
        <property type="entry name" value="AcylCoA_DH/ox_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037069">
        <property type="entry name" value="AcylCoA_DH/ox_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009100">
        <property type="entry name" value="AcylCoA_DH/oxidase_NM_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00441">
        <property type="entry name" value="Acyl-CoA_dh_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02770">
        <property type="entry name" value="Acyl-CoA_dh_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02771">
        <property type="entry name" value="Acyl-CoA_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47203">
        <property type="entry name" value="SSF47203"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56645">
        <property type="entry name" value="SSF56645"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00072">
        <property type="entry name" value="ACYL_COA_DH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00073">
        <property type="entry name" value="ACYL_COA_DH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0274">FAD</keyword>
    <keyword evidence="3" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-0614">Plasmid</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Acyl-CoA_dh_N" evidence="7">
        <location>
            <begin position="11"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA_dh_M" evidence="6">
        <location>
            <begin position="126"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA_dh_1" evidence="5">
        <location>
            <begin position="233"/>
            <end position="380"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF02770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF02771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ABF12296.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="387" mass="43128" checksum="9CEC9ED88E8ECC00" modified="2006-05-30" version="1">MSQTRRLIFDADHEQFRASVARFVQNEITPYVDQWREQGCCDRAIFEKAGEQGLLLMWADEAYGGAGVTDFRYEQILFEELIRHGDVGVYLTLHSRLVAPYIGRLGTEAQKRRWLTGAASGKTILAIAMTEPGAGSDLAGIGARAERRDGGWVINGAKTFISNGHNADAVIVVARTNPDERRAFGLFVVERGMAGFERGRRLKKLGLDAQDTAELFFNDVFVPDENVLGNPARGFAYLAEGLEEERLLCACQSIAHAQSAFDLTLPYVKERRAFGRPIGALQNTRFVLARRRAELDAMQIWLDALVMEHNAGRLDAVTAASAKLLSTELENRVIDDCLQLHGGSGYMDEYRISRMYRDARVSRIFAGASEIMLEIISRSLGLTERMD</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="49" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LC40</accession>
    <name>Q1LC40_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_5427</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12286.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008641308.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF12286">
        <property type="protein sequence ID" value="ABF12286"/>
        <property type="gene ID" value="Rmet_5427"/>
    </dbReference>
    <dbReference type="GeneID" id="60826363"/>
    <dbReference type="KEGG" id="rme:Rmet_5427"/>
    <dbReference type="HOGENOM" id="CLU_2525165_0_0_4"/>
    <dbReference type="OMA" id="RHVTATY"/>
    <dbReference type="OrthoDB" id="1848842at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF12286.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="84" mass="9356" checksum="2E2A2467FF5B7A53" modified="2006-05-30" version="1">MPNNEEWEELHLTPAGWIAGSYRHTPWQPVAVTPPDAAVLTVRRHVTATYGGPSRAIEDRTPQTQDMALIESLLTRYGSPEFGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="60" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LCC3</accession>
    <name>Q1LCC3_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_5344</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12203.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519748.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF12203">
        <property type="protein sequence ID" value="ABF12203"/>
        <property type="gene ID" value="Rmet_5344"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5344"/>
    <dbReference type="eggNOG" id="ENOG5031UYB">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1493511_0_0_4"/>
    <dbReference type="OMA" id="INWIYLP"/>
    <dbReference type="OrthoDB" id="1827812at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="49"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="82"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="119"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="146"/>
            <end position="169"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF12203.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="197" mass="21862" checksum="C1969EE3A845B0BC" modified="2006-05-30" version="1">MPHLRLQFAMVLATIVMFALTLAANDWLFPQLELIPGINWIYLPAGVRLVATLLFGGAGALGLLIVSWLVCFLYLFPDDFSRAFMGGILATVAPYLVYKSAQHWFGLRASLANLTPKRLLACIVAYSVASPLLHHIWFYLHDGADHVVQSFFVMFIGDLSGTLIVIYTIKGLLALMPRRPRADAQCTFPEQSAGHRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="68" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LCF3</accession>
    <name>Q1LCF3_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Rmet_5314</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="8">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="8">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the DoxX family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12173.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519718.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5314"/>
    <dbReference type="EnsemblBacteria" id="ABF12173">
        <property type="protein sequence ID" value="ABF12173"/>
        <property type="gene ID" value="Rmet_5314"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5314"/>
    <dbReference type="eggNOG" id="COG2259">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_058421_11_0_4"/>
    <dbReference type="OMA" id="IVIVHTF"/>
    <dbReference type="OrthoDB" id="2084223at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032808">
        <property type="entry name" value="DoxX"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07681">
        <property type="entry name" value="DoxX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0614">Plasmid</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="21"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="60"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="86"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="114"/>
            <end position="135"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABF12173.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="140" mass="14793" checksum="635A7FBBA29640A5" modified="2006-05-30" version="1">MPSSMPSLSSAGDSPHRLPRWLHWVALLLLCAAYLQGGLVKLFDFPSAMAEMQHFGLSPAGPLAAGTIALELGASAMILSGRGRWLGALALAAFTLAATFLANSFWSAPPDARFGMANAFFEHLGLVGGFVLVAWHDLRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LCF5</accession>
    <name>Q1LCF5_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Non-heme chloroperoxidase (Chloride peroxidase) (CPO-F) (Chloroperoxidase F)</fullName>
            <ecNumber evidence="2">1.11.1.10</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">cpo</name>
        <name evidence="2" type="ordered locus">Rmet_5312</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EC" id="1.11.1.10" evidence="2"/>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12171.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519716.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5312"/>
    <dbReference type="ESTHER" id="ralme-q1lcf5">
        <property type="family name" value="Haloperoxidase"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF12171">
        <property type="protein sequence ID" value="ABF12171"/>
        <property type="gene ID" value="Rmet_5312"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5312"/>
    <dbReference type="eggNOG" id="COG2267">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020336_12_3_4"/>
    <dbReference type="OMA" id="EDQMLFL"/>
    <dbReference type="OrthoDB" id="1282004at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016691">
        <property type="term" value="F:chloride peroxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000639">
        <property type="entry name" value="Epox_hydrolase-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00561">
        <property type="entry name" value="Abhydrolase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00111">
        <property type="entry name" value="ABHYDROLASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00412">
        <property type="entry name" value="EPOXHYDRLASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="SSF53474"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="2" id="KW-0575">Peroxidase</keyword>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AB hydrolase-1" evidence="1">
        <location>
            <begin position="22"/>
            <end position="257"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF12171.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="273" mass="29373" checksum="AB3B787AF541BF4D" modified="2006-05-30" version="1">MSYITAEDGTQIFYKDWGSGRPVVFSHGWPLNADAWDAQMLFLVQKGFRVIAHDRRGHGRSDQPARGNDMDTYADDLAALLVALDIKGATLVGHSTGGGEVARYIGRHGTQRVAKAVLIGAVPPIMAKTASNPNGLPIDVFDGIRKGVADNRSQFYKDLAVPFFGFNRPGAKVSQGTIDTFWAQGMAGGIVGQYACIHEFSEVDYTEDLKKIDVPTLVLHGDDDQIVPIDNAGRLSAKLVKNATLKVYPGASHGMCVVNADAVNADLLAFLNT</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-10-07" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LCI9</accession>
    <name>Q1LCI9_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Extra-cytoplasmic solute receptor protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">bug</name>
        <name evidence="4" type="ordered locus">Rmet_5278</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="5">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="5">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Periplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the UPF0065 (bug) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12137.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5278"/>
    <dbReference type="EnsemblBacteria" id="ABF12137">
        <property type="protein sequence ID" value="ABF12137"/>
        <property type="gene ID" value="Rmet_5278"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5278"/>
    <dbReference type="eggNOG" id="COG3181">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045683_0_0_4"/>
    <dbReference type="OMA" id="ATVAHTM"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042597">
        <property type="term" value="C:periplasmic space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005064">
        <property type="entry name" value="BUG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042100">
        <property type="entry name" value="Bug_dom1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006311">
        <property type="entry name" value="TAT_signal"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42928">
        <property type="entry name" value="PTHR42928"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03401">
        <property type="entry name" value="TctC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017082">
        <property type="entry name" value="YflP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51318">
        <property type="entry name" value="TAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-0675">Receptor</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193337" evidence="3">
        <location>
            <begin position="32"/>
            <end position="331"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF12137.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="331" mass="34547" checksum="6FDFDCA5D8A2D8DD" modified="2006-05-30" version="1">MTPCMARRTALRMLAGAAIVVAAVPTGSAFAADHWPSKPITLVVPFASGGTTDIIGRAVGQRLGEALGQPVVVDNRPGAGGTIGGALVARANPDGYTFLLATVAHTMAPGIYKTLPYDFQKDLEPIGMVALTPNVVLVNTSIPARNVQELIAYIKANPGKVNYGSAGIGSTEHLSGELFRSATGTDISHVPYKGGAPMMTDLIAGQIQMAIETSPSANPHVKSGKVKALAVTSAKRSPAYPGVPTVAESGVPGYEVTTWYALMAPRGTPEPIRQRMSAELAKVLKQSDVQKRFDDQGVTAGDMTPPQLAAFIKTETTKWTKVAKDSGAKAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LCS6</accession>
    <name>Q1LCS6_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Amino acid permease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Rmet_5191</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="9">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="9">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12050.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519597.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5191"/>
    <dbReference type="EnsemblBacteria" id="ABF12050">
        <property type="protein sequence ID" value="ABF12050"/>
        <property type="gene ID" value="Rmet_5191"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5191"/>
    <dbReference type="eggNOG" id="COG1113">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007946_9_3_4"/>
    <dbReference type="OMA" id="FSMLKIM"/>
    <dbReference type="OrthoDB" id="430372at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006865">
        <property type="term" value="P:amino acid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004841">
        <property type="entry name" value="AA-permease/SLC12A_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004840">
        <property type="entry name" value="Amoino_acid_permease_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00324">
        <property type="entry name" value="AA_permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00218">
        <property type="entry name" value="AMINO_ACID_PERMEASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0614">Plasmid</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="26"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="55"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="103"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="134"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="167"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="206"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="251"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="290"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="342"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="366"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="402"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="432"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="domain" description="AA_permease" evidence="7">
        <location>
            <begin position="26"/>
            <end position="431"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ABF12050.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="475" mass="51222" checksum="4770F7F682A652FB" modified="2006-05-30" version="1">MRQQQGFDAIVEREQGLHRGLSTGQLSMIAIGGAIGTGLFLGSGFAIGFAGPSVLVSYAIGALIALLLMGCLAEMTVAHPTSGSFGANAEHYIGPLAGFLVRYAYWSSIVFAVGTEVTAIAVYMKYWFPMVPGWYWIVGFSAALIGINATSVKVFGAVEYVFSMLKIVAIVGFILLGAYVVTRAPTGSGIGLANYTAHGGFFPKGMWGTWVAVIVSIFSYLSIEMIAVAAGEARDPETAITRAFRATMVRLVFFYLLTLALMLMIVPWTAAGAGESPFVRVMAATRVPGAAAVFNFVILVAALSAMNSQLYITTRMMFSLSRAGYAPKALGQVNGRGVPMTALLLSTVGIAFAVVLNVVYPDASFMLMMAISMFGAMFTWLMIFITHLFFRRQHRGRPLAFRMWGFPVTTLLGAGLMFATLVTTWFTPEFRMTLVAGVPFMLALSAAYWLWYRKRGGAAQGDGEDAGRAEVRNSW</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="64" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LD38</accession>
    <name>Q1LD38_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_5079</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11938.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008652041.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5079"/>
    <dbReference type="EnsemblBacteria" id="ABF11938">
        <property type="protein sequence ID" value="ABF11938"/>
        <property type="gene ID" value="Rmet_5079"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5079"/>
    <dbReference type="eggNOG" id="COG3246">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_065536_2_0_4"/>
    <dbReference type="OMA" id="MEDPAVF"/>
    <dbReference type="OrthoDB" id="1892901at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019475">
        <property type="term" value="P:L-lysine catabolic process to acetate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008567">
        <property type="entry name" value="KCE_family"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37418">
        <property type="entry name" value="PTHR37418"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05853">
        <property type="entry name" value="BKACE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF11938.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="352" mass="38746" checksum="C6C96510062815DE" modified="2006-05-30" version="1">MNFLDGHLFPENQQPLIITAAPYAPSWVPSDFPEEIPVTMEEQIQKAVDCYNAGATVLHLHVRELDGKGSKRLSKFNELIAGVRARVPEMIIQVGGSISFAPETEGAAAKWLSDDTRHMLAELDPKPDQVTVTVNTSQMNVLEQFDHRDIVGSSMEDPAVFNAYKEMTVPAQPGWVEEHVRRLTAAGIQSAFQCYNVNSFESVERLMRRGIYKGPLVMNWVAIGGGMDSPTVYSLANFMRGVPDGAVVTVESSVLNVLPVNTIGIAMGLHVRCGTEDNLWNQTRTKKIGTAAQIEQLVRISRELSRPIATAKEAREMCKIGVFYDTVEESLEANGFAPNRNGGRQGFLQKAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LD76</accession>
    <name>Q1LD76_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Extra-cytoplasmic solute receptor protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">bug</name>
        <name evidence="4" type="ordered locus">Rmet_5038</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="5">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="5">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Periplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the UPF0065 (bug) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11900.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5038"/>
    <dbReference type="EnsemblBacteria" id="ABF11900">
        <property type="protein sequence ID" value="ABF11900"/>
        <property type="gene ID" value="Rmet_5038"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5038"/>
    <dbReference type="eggNOG" id="COG3181">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045683_0_0_4"/>
    <dbReference type="OMA" id="YSWIGIF"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042597">
        <property type="term" value="C:periplasmic space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005064">
        <property type="entry name" value="BUG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042100">
        <property type="entry name" value="Bug_dom1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42928">
        <property type="entry name" value="PTHR42928"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03401">
        <property type="entry name" value="TctC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017082">
        <property type="entry name" value="YflP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-0675">Receptor</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF11900.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="348" mass="36754" checksum="36F9EE414909D95A" modified="2006-05-30" version="1">MLFPCFSSRRQTTPAASGGMPRAQRGRLAVIAALSALCLPGGMLPTSAWADSGTPLKLVVTFPPGGSTDIAARIVQPKLAEVLGRPVVVENRPGAASQVATQYVARSAPDGNTLLISFDTHAINPIAKSRLPYDTFKDFSGVTLALRFPLVIGAHPSVPGKDLRGFLDAARRAPNQYSYASTGLGSMNHLVAEDLKRQAGVELLHVPYAGGGPAVQAVLGNVSSLTLLSYAALKGQIAAGRIKPLAVTGANRLPDLPDVPTVAESGFPGFEAYSWIGVFAPSGTPPAVARKLTSDFQAALNDPETHRKLTQAGFEVMATDGPALDRYAREQYERWKAFVVKTGLKLEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LDB5</accession>
    <name>Q1LDB5_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Rmet_4999</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="5">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="5">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11861.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519411.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4999"/>
    <dbReference type="EnsemblBacteria" id="ABF11861">
        <property type="protein sequence ID" value="ABF11861"/>
        <property type="gene ID" value="Rmet_4999"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4999"/>
    <dbReference type="eggNOG" id="COG5361">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027269_2_0_4"/>
    <dbReference type="OMA" id="MHTTGAG"/>
    <dbReference type="OrthoDB" id="547506at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.600">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1610">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010621">
        <property type="entry name" value="DUF1214"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037049">
        <property type="entry name" value="DUF1214_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010679">
        <property type="entry name" value="DUF1254"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037050">
        <property type="entry name" value="DUF1254_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06742">
        <property type="entry name" value="DUF1214"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06863">
        <property type="entry name" value="DUF1254"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0614">Plasmid</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193335" evidence="1">
        <location>
            <begin position="25"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="domain" description="DUF1254" evidence="3">
        <location>
            <begin position="88"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="domain" description="DUF1214" evidence="2">
        <location>
            <begin position="384"/>
            <end position="490"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF06742"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF06863"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF11861.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="506" mass="55875" checksum="A1E0CE3E1425AD74" modified="2006-05-30" version="1">MKELRWTSIALAALALGLSGAAIAQSSRYDALANAAFEKDYPTPETSRMLQEELLFQRATQAYLWALPAINIWAMKEGSEKVFGGGYNVLPVWKERIKASTQVTTPNSDVVYAMGYLDLKKDGPIVVEAPAGVQGILDDFFQRPLVGPTTEGRTWTGDVGLAGPDKGKGGTYVLLPPDYKGEPPKGSLPKGAFVYRSRTYNVFLFWRTFFTDPKDLSKPNAQIAATRIYPLGQKASARPMQFPDANGKPADLLFPRDASYFDMMSRFIDSEYVDPSDLDMRGFLHTIGIEKGKPFSPSPEMRSLLERAAQTGFKISKVVIADMVVKEPGGKYYADRQWVNVFAGEDTSFQSSRTYTNFEQRSGYFTSAYSASPGMVKNIVNAGAKYPVTSRDKDGNFLDGGQSYTLHLPPNIPAKNFWSATVYDGTTASGLDNGQSHPSLNQMDKPVQNPDGSTNLYFGPTAPAGKEKNWLRTVPGKGYFVILRLYSPEKAFFDQTWKPGDIEKVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="58" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LDB8</accession>
    <name>Q1LDB8_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_4996</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11858.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519407.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF11858">
        <property type="protein sequence ID" value="ABF11858"/>
        <property type="gene ID" value="Rmet_4996"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4996"/>
    <dbReference type="eggNOG" id="ENOG50330AH">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_101709_1_1_4"/>
    <dbReference type="OMA" id="VKYQADY"/>
    <dbReference type="OrthoDB" id="1400203at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021851">
        <property type="entry name" value="DUF3455"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11937">
        <property type="entry name" value="DUF3455"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193023" evidence="1">
        <location>
            <begin position="38"/>
            <end position="184"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF11858.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="184" mass="19234" checksum="5CED1FD9EE30361B" modified="2006-05-30" version="1">MNQSIRRGSRGVRLATRRAFAIATALAGSVALSLAQAQSPVPGVLQPAGMTHAVASLEASGVQIYVCRRDAANQLTWTFQAPQADLYDASGQLVVRHGAGPSWEALDGSRITGKLMQHAANPDDSAAIPMLLLSSTNAGGPGLLSTVRYVQRLATHGGAAPAQACVHEGEEGRSPYLAEYVFLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="96" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LDM3</accession>
    <name>Q1LDM3_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Sulfite:cytochrome c oxidoreductase subunit A</fullName>
            <ecNumber evidence="7">1.8.2.1</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">sorA</name>
        <name evidence="7" type="ordered locus">Rmet_4891</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="8">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="8">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mo-molybdopterin</name>
            <dbReference type="ChEBI" id="CHEBI:71302"/>
        </cofactor>
    </comment>
    <dbReference type="EC" id="1.8.2.1" evidence="7"/>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11753.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519313.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4891"/>
    <dbReference type="EnsemblBacteria" id="ABF11753">
        <property type="protein sequence ID" value="ABF11753"/>
        <property type="gene ID" value="Rmet_4891"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4891"/>
    <dbReference type="eggNOG" id="COG2041">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003827_5_5_4"/>
    <dbReference type="OMA" id="WARHVQF"/>
    <dbReference type="OrthoDB" id="1345710at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030151">
        <property type="term" value="F:molybdenum ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050310">
        <property type="term" value="F:sulfite dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.420.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005066">
        <property type="entry name" value="MoCF_OxRdtse_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008335">
        <property type="entry name" value="Mopterin_OxRdtase_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000572">
        <property type="entry name" value="OxRdtase_Mopterin-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036374">
        <property type="entry name" value="OxRdtase_Mopterin-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006311">
        <property type="entry name" value="TAT_signal"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03404">
        <property type="entry name" value="Mo-co_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00174">
        <property type="entry name" value="Oxidored_molyb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00407">
        <property type="entry name" value="EUMOPTERIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56524">
        <property type="entry name" value="SSF56524"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="SSF81296"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51318">
        <property type="entry name" value="TAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0500">Molybdenum</keyword>
    <keyword evidence="3 7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-0614">Plasmid</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193375" evidence="4">
        <location>
            <begin position="33"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="domain" description="Oxidored_molyb" evidence="5">
        <location>
            <begin position="96"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="domain" description="Mo-co_dimer" evidence="6">
        <location>
            <begin position="293"/>
            <end position="401"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001924"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022505"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF03404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABF11753.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="410" mass="44686" checksum="40378D11467B7A97" modified="2006-05-30" version="1">MENRVRRRRFLKQAGALTANAVVGTTALNALAASGDKQVQLPFDNGARELVAFPQKRPLILLTSRPPQLETPFSVFNEGILTPNDAFFVRYHWSGIPTSVDPQTYRLRIGGKVKTPLELSLADIKAIAQPMEVVAVNQCSGNSRGFLAPRVNGGQLANGAMGNARWTGVPLKAVLERAGVQPGAVQVSFNGLDRPPLDNSPDFVKSLDIDHALDGEVMLAWSMNGEDLPMLNGYPLRLVVPGHYGTYWVKHLSDITVLDKPFDGFWVQSAYRIPDNAGANVEPGSAPARTRPIGRFNVRSFITSLTEGATVPSGRETVVRGIAFDGGYGIAEVAFSADGGRSWTEATLGQDLGKYSFREWRAAFRPPRKGAYDLRVRAVNRIGQSQPMTALWNPAGYMRNVVETTRVKAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LDX5</accession>
    <name>Q1LDX5_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Lipoprotein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">metQ</name>
        <name evidence="6" type="ordered locus">Rmet_4789</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="7">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="7">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the nlpA lipoprotein family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11651.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008651202.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4789"/>
    <dbReference type="EnsemblBacteria" id="ABF11651">
        <property type="protein sequence ID" value="ABF11651"/>
        <property type="gene ID" value="Rmet_4789"/>
    </dbReference>
    <dbReference type="GeneID" id="60821623"/>
    <dbReference type="KEGG" id="rme:Rmet_4789"/>
    <dbReference type="eggNOG" id="COG1464">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_067080_3_0_4"/>
    <dbReference type="OMA" id="YQDDAES"/>
    <dbReference type="OrthoDB" id="1230456at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004872">
        <property type="entry name" value="Lipoprotein_NlpA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30429">
        <property type="entry name" value="PTHR30429"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03180">
        <property type="entry name" value="Lipoprotein_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002854">
        <property type="entry name" value="MetQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00363">
        <property type="entry name" value="TIGR00363"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0614">Plasmid</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193046" description="Lipoprotein" evidence="5">
        <location>
            <begin position="28"/>
            <end position="270"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRNR" id="PIRNR002854"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABF11651.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="270" mass="28854" checksum="B98A11401F171FD9" modified="2006-05-30" version="1">MKMLSISKTLAAATVALGIVAAGNAAAQEQTIRVGTVSGPDAEVWQVVQKVAKKNGLNVKVVEFNDYVQPNAALDAGDLDANSFQHQPYLDSQIKQRGYKMTSVGYTYISPLGIYSKNLKSSKDLPQGAKVAVPNDPSNENRALLLLAAQGAIKLKPGVGTNGVNATPLDVAENPKKLKIVELDAAQLARALPDVAAAVINTNYALAAGLQPTKDAIALEDIHSPYANIIVVRTQDKDKPWVKKLVAAYQSEEVRQFMKAQYKGAMVPSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="98" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LE41</accession>
    <name>Q1LE41_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Transcriptional regulator, TetR family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Rmet_4723</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="8">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="8">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11585.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4723"/>
    <dbReference type="EnsemblBacteria" id="ABF11585">
        <property type="protein sequence ID" value="ABF11585"/>
        <property type="gene ID" value="Rmet_4723"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4723"/>
    <dbReference type="eggNOG" id="COG1309">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069356_40_0_4"/>
    <dbReference type="OMA" id="VAFACAQ"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001647">
        <property type="entry name" value="HTH_TetR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036271">
        <property type="entry name" value="Tet_transcr_reg_TetR-rel_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025996">
        <property type="entry name" value="TetR_C_WHG"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13305">
        <property type="entry name" value="TetR_C_33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00440">
        <property type="entry name" value="TetR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48498">
        <property type="entry name" value="SSF48498"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50977">
        <property type="entry name" value="HTH_TETR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-0614">Plasmid</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH tetR-type" evidence="6">
        <location>
            <begin position="33"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="H-T-H motif" evidence="4">
        <location>
            <begin position="56"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABF11585.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="230" mass="24420" checksum="F5C98C12162772EA" modified="2006-05-30" version="1">MNRTAAPTPPTPPTPPTPPTPPTPPTRTTYRHGDLRRALLEAGIDLARQGGPDAVVLREATRRAGVVPNAAYRHFANRQDLLQAVRAHALSGLAMAMEAELATIPSDAAPAERARASLRAVGAGYLRFALGETGLFRTAFSVPDDVEEDSDPAKAGNSGLNPFELLGAALDRWVEAGLLPVERRPGAEYLAWSAVHGLAILLIDGPLRGRAREQAGVLGQRLLDLVEKGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LEH5</accession>
    <name>Q1LEH5_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">PLP-dependent aminotransferase, UDP-4-amino-4-deoxy-L-arabinose--oxoglutarate aminotransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">arnB</name>
        <name evidence="4" type="ordered locus">Rmet_4589</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="5">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="5">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the DegT/DnrJ/EryC1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11451.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008648543.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4589"/>
    <dbReference type="EnsemblBacteria" id="ABF11451">
        <property type="protein sequence ID" value="ABF11451"/>
        <property type="gene ID" value="Rmet_4589"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4589"/>
    <dbReference type="eggNOG" id="COG0399">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033332_7_2_4"/>
    <dbReference type="OMA" id="QVHYKPI"/>
    <dbReference type="OrthoDB" id="1722208at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008483">
        <property type="term" value="F:transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00616">
        <property type="entry name" value="AHBA_syn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000653">
        <property type="entry name" value="DegT/StrS_aminotransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30244">
        <property type="entry name" value="PTHR30244"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01041">
        <property type="entry name" value="DegT_DnrJ_EryC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000390">
        <property type="entry name" value="PLP_StrS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="SSF53383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="4" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2 3" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="active site" description="Proton acceptor" evidence="1">
        <location>
            <position position="188"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="2">
        <location>
            <position position="188"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRSR" id="PIRSR000390-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR000390-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU004508"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF11451.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="391" mass="42586" checksum="D7ED2B8AF7EB5D73" modified="2006-05-30" version="1">MTASAPQDFLPFVRPNIDDATIAEVGKVLASGWITSGPKVQAFEATLSDLFGGRPVRTFSNGSATMEIALRIADIGPGDEVITTPITWVATANVVLTVGARPVFVDIDPRTRNIDLDAVEAAITPRTRAIIPVYLSGLPVDMDRLYAIAKRHNLRVIEDAAQAIDSRWRGQRIGAFGDLVSFSFQANKNITTIEGGCLVMNSPEEAQRAERLRLQGVIRTGMDGMDVEEPGGKFNLTDVNAAIGLAQLQHLDAITARRAELAETYFRCAANAGLADLGIELPLPRDPDVATTNWHMFQVVLPTDRLRGGAAEARKHVMESMRESGIGTGVHYPPVHLFTYFRSLGWREGMLPHAERIGRGIVTLPMFPAMQTADVERVCATLSETCKRLSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="92" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LEL8</accession>
    <name>Q1LEL8_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Transcriptional regulator, LysR family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_4543</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the LysR transcriptional regulatory family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11408.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518999.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4543"/>
    <dbReference type="EnsemblBacteria" id="ABF11408">
        <property type="protein sequence ID" value="ABF11408"/>
        <property type="gene ID" value="Rmet_4543"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4543"/>
    <dbReference type="eggNOG" id="COG0583">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039613_16_3_4"/>
    <dbReference type="OMA" id="LYPRNRQ"/>
    <dbReference type="OrthoDB" id="750230at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005119">
        <property type="entry name" value="LysR_subst-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000847">
        <property type="entry name" value="Tscrpt_reg_HTH_LysR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00126">
        <property type="entry name" value="HTH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03466">
        <property type="entry name" value="LysR_substrate"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50931">
        <property type="entry name" value="HTH_LYSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH lysR-type" evidence="2">
        <location>
            <begin position="1"/>
            <end position="59"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF11408.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="304" mass="33472" checksum="E1C9B28147BCB1F4" modified="2006-05-30" version="1">MDRIDAMRVFTRIVERRSFTAAAKDLDLPRSSVTDAVKQLESRLGVRLLQRTTRHVSPTLDGEAYFQRCLRLLADFEEAEAAFAGTSPKGVLRVDVQGQLARRFLFPALPAFLAQHPGITLQISEGDRLVDLVREGIDCVLRVGEPQDSEMVARRVARLAQLTLAAPPYLARHGSPVSIESLGNHRMVGFRSTLTGGLLPLEFMTGGVRSEVMLPAPVSVSGAESLVAACRLGLGLIQVPRYHVEEDLLRGTLVSVLSEIPPPTMSVSLLYPRSRQLSQRVRVFLDWVSAEFAKHRGKNGVEVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="104" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LEP5</accession>
    <name>Q1LEP5_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Two component transcriptional regulator, LuxR family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Rmet_4516</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="6">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="6">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11381.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518974.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4516"/>
    <dbReference type="EnsemblBacteria" id="ABF11381">
        <property type="protein sequence ID" value="ABF11381"/>
        <property type="gene ID" value="Rmet_4516"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4516"/>
    <dbReference type="eggNOG" id="COG2197">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_90_1_4"/>
    <dbReference type="OMA" id="HQGNANF"/>
    <dbReference type="OrthoDB" id="1687028at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06170">
        <property type="entry name" value="LuxR_C_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016032">
        <property type="entry name" value="Sig_transdc_resp-reg_C-effctor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000792">
        <property type="entry name" value="Tscrpt_reg_LuxR_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00196">
        <property type="entry name" value="GerE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00038">
        <property type="entry name" value="HTHLUXR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00421">
        <property type="entry name" value="HTH_LUXR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46894">
        <property type="entry name" value="SSF46894"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50043">
        <property type="entry name" value="HTH_LUXR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="5" id="KW-0614">Plasmid</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Response regulatory" evidence="4">
        <location>
            <begin position="3"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="domain" description="HTH luxR-type" evidence="3">
        <location>
            <begin position="141"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="2">
        <location>
            <position position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABF11381.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="214" mass="23679" checksum="39C89A35832B4D00" modified="2006-05-30" version="1">MIRVIIADDHTVVRNGLRHILERTSDFEVVGEAARGTEVPQLVRELSPHVILLDLSMPGRNGLELIRQLRTDHPALRILVLTMHAEEQYVVRAFRAGAAGYLTKESAATELVEALRKVAAGGTYVSPAMAEKLALGLQEKPDAPHEGLSDRELEVFRRIVDGESLTQIAAALCVSAKTVSTYKMRLMEKLQLRSDAALVRYAIEARLFGDEDKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LES7</accession>
    <name>Q1LES7_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Bifunctional deaminase-reductase-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_4484</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11349.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518947.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4484"/>
    <dbReference type="EnsemblBacteria" id="ABF11349">
        <property type="protein sequence ID" value="ABF11349"/>
        <property type="gene ID" value="Rmet_4484"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4484"/>
    <dbReference type="eggNOG" id="COG0262">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_043966_3_0_4"/>
    <dbReference type="OMA" id="HHLFWRR"/>
    <dbReference type="OrthoDB" id="1920912at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008703">
        <property type="term" value="F:5-amino-6-(5-phosphoribosylamino)uracil reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009231">
        <property type="term" value="P:riboflavin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.430.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024072">
        <property type="entry name" value="DHFR-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002734">
        <property type="entry name" value="RibDG_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01872">
        <property type="entry name" value="RibD_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53597">
        <property type="entry name" value="SSF53597"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RibD_C" evidence="1">
        <location>
            <begin position="9"/>
            <end position="208"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01872"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF11349.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="214" mass="23525" checksum="50C0C14A4F9EF28C" modified="2006-05-30" version="1">MARVRVNGFTISLDGYGAGPNQDINNPLGIGGTELHQWLIPTRTFQRSLFGKDDGTTGIDDDFAARGFQNVGAWILGRNMFGPIRGDWPDMNWNGWWGDSPPYHVPVFVLTHHPRPSVEMKGGTTFHFITGGIREALDRAREAAAGMDVRIGGGPETIRQYLRDGLIDELHIAISPVLLGQGESLFEGIDLRALGYTCVESVASEKATHVILRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-10-07" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LEW9</accession>
    <name>Q1LEW9_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Transcriptional regulator, LysR family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_4442</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the LysR transcriptional regulatory family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11307.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4442"/>
    <dbReference type="EnsemblBacteria" id="ABF11307">
        <property type="protein sequence ID" value="ABF11307"/>
        <property type="gene ID" value="Rmet_4442"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4442"/>
    <dbReference type="eggNOG" id="COG0583">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039613_6_3_4"/>
    <dbReference type="OMA" id="VQTYYMA"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005119">
        <property type="entry name" value="LysR_subst-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000847">
        <property type="entry name" value="Tscrpt_reg_HTH_LysR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00126">
        <property type="entry name" value="HTH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03466">
        <property type="entry name" value="LysR_substrate"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00039">
        <property type="entry name" value="HTHLYSR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50931">
        <property type="entry name" value="HTH_LYSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH lysR-type" evidence="2">
        <location>
            <begin position="32"/>
            <end position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF11307.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="334" mass="36142" checksum="F49D2424657C31B7" modified="2006-05-30" version="1">MPKTGVTAVTGVFRPLVRYTAAQCRRSSPRAMRLRHLEIFHAVMRTGSLSRAAELLFISQPAASKVLAHAERDLGFKLFTRAHGRLLPTREAQLLHAETVHLFANLENVRQLARNLRRHPEGHLRIGCLPSLGLSLIPQAVREFRQRCPGVSLEIETRHGDELQGLVMSRELDIAVVFDPAPRPGVTSSALGQAEVVYLGPASNAPPHGPVQLAALDDQHWIGIGNSDPLGGLIAQAFRDLGLEERTPMIEARTYYVAAALAECGVGYALVDHFTAQFSQRASGEELLIRPLEPALSVGVAAIYADGAASSHAHDTLLAILGSQFEAALPGRPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LF26</accession>
    <name>Q1LF26_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Outer membrane lipoprotein carrier protein LolA</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">lolA</name>
        <name evidence="3" type="ordered locus">Rmet_4385</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11250.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518867.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4385"/>
    <dbReference type="EnsemblBacteria" id="ABF11250">
        <property type="protein sequence ID" value="ABF11250"/>
        <property type="gene ID" value="Rmet_4385"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4385"/>
    <dbReference type="eggNOG" id="COG2834">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_091014_0_0_4"/>
    <dbReference type="OMA" id="NGTAWEN"/>
    <dbReference type="OrthoDB" id="1933827at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="CDD" id="cd16325">
        <property type="entry name" value="LolA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029046">
        <property type="entry name" value="LolA/LolB/LppX"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004564">
        <property type="entry name" value="OM_lipoprot_carrier_LolA-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03548">
        <property type="entry name" value="LolA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89392">
        <property type="entry name" value="SSF89392"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193134" evidence="2">
        <location>
            <begin position="27"/>
            <end position="209"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF11250.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="209" mass="22762" checksum="D292D3042BEF146E" modified="2006-05-30" version="1">MMRRRALQATFALVTMAAMSVVPAHAEDLSAAVSARLSDAPVIHGQFEQTRRLAGFSNPLISRGDFVMARGRGVVWATREPFASSLLVTPEKLVMRGADGKVQQEMQADAQPAMRLVGESMIAILRGDLRSLSTRFSVTGKLVGKSGWTLTLTPTDAGVRRAFTRIDLAGDRYVRDVRLEEASGDSTVVRMIDPAGAEKLSPSEEQRFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="64" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LF62</accession>
    <name>Q1LF62_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_4349</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11214.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518834.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF11214">
        <property type="protein sequence ID" value="ABF11214"/>
        <property type="gene ID" value="Rmet_4349"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4349"/>
    <dbReference type="eggNOG" id="COG3171">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_153063_1_0_4"/>
    <dbReference type="OMA" id="PLLDVWY"/>
    <dbReference type="OrthoDB" id="1513915at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007416">
        <property type="entry name" value="YggL_50S_bp"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38778">
        <property type="entry name" value="PTHR38778"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04320">
        <property type="entry name" value="YggL_50S_bp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF11214.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="116" mass="12787" checksum="33DFFC4696F31F50" modified="2006-05-30" version="1">MSQRRSRRQRKKLRVAEFQELGFLVTAELAVGLGADAKIAACEAFIADCIEANQLTYGGAIDDRLDGFVSPEGNRSSSTEEHRRIVLDWLNGRAEFGAVRVGPLIDAWYGNFAELG</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="55" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LFN6</accession>
    <name>Q1LFN6_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_4173</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11040.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518661.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4173"/>
    <dbReference type="EnsemblBacteria" id="ABF11040">
        <property type="protein sequence ID" value="ABF11040"/>
        <property type="gene ID" value="Rmet_4173"/>
    </dbReference>
    <dbReference type="GeneID" id="60823246"/>
    <dbReference type="KEGG" id="rme:Rmet_4173"/>
    <dbReference type="HOGENOM" id="CLU_2424765_0_0_4"/>
    <dbReference type="OMA" id="RGTECVC"/>
    <dbReference type="OrthoDB" id="1845791at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF11040.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="91" mass="10511" checksum="1A26DCD5F5FC05A6" modified="2006-05-30" version="1">MDKPTVSPRRFVEILNERLTRHPLYREGMHVYAIPPHSEHPHSLVCVGPRGTECVCTTIEAIVRGECDVFPDIARDWRPRPNPPAHEHRAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-12-02" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LFP4</accession>
    <name>Q1LFP4_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Lipoprotein vacJ</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Rmet_4165</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="5">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="5">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the MlaA family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11032.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4165"/>
    <dbReference type="EnsemblBacteria" id="ABF11032">
        <property type="protein sequence ID" value="ABF11032"/>
        <property type="gene ID" value="Rmet_4165"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4165"/>
    <dbReference type="eggNOG" id="COG2853">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_059326_1_0_4"/>
    <dbReference type="OMA" id="PYRYIDH"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007428">
        <property type="entry name" value="MlaA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30035">
        <property type="entry name" value="PTHR30035"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04333">
        <property type="entry name" value="MlaA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01805">
        <property type="entry name" value="VACJLIPOPROT"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="4" id="KW-0614">Plasmid</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="244"/>
            <end position="291"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF11032.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="291" mass="30519" checksum="7A34E060806440FC" modified="2006-05-30" version="1">MLSRFSPSGAVALRQPQLARTLAAVSLSVLATGCATGPQANPQDPLEPMNRVIFKVNDTIDTYGAQPLAKGYKAVTPEPARLAVTNFFSNLSDVGNFVNNVLQGKGVDATETMMRIAINSVLGIGGLIDVATAAGLNRHSQDFGLTLGTWGVPAGPYLVLPIYGPSSFRDSMGVAASIYLDPAHYLDPAWRNSLFGVNVINTRSNLLGATDLLSQAALDKYAFVRDAYSQRRQYLLNQGENALPSYDEVDESDKASEAKPAAGEAAPATPATPATPATPATPAAPPSDSAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="106" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LG01</accession>
    <name>Q1LG01_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Cytochrome c-type biogenesis protein CcmE</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">Cytochrome c maturation protein E</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="4">Heme chaperone CcmE</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="4 7" type="primary">ccmE</name>
        <name evidence="4" type="synonym">cycJ</name>
        <name evidence="7" type="ordered locus">Rmet_4057</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="8">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="8">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Heme chaperone required for the biogenesis of c-type cytochromes. Transiently binds heme delivered by CcmC and transfers the heme to apo-cytochromes in a process facilitated by CcmF and CcmH.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cell inner membrane</location>
            <topology evidence="4">Single-pass type II membrane protein</topology>
            <orientation evidence="4">Periplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the CcmE/CycJ family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10925.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518552.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4057"/>
    <dbReference type="EnsemblBacteria" id="ABF10925">
        <property type="protein sequence ID" value="ABF10925"/>
        <property type="gene ID" value="Rmet_4057"/>
    </dbReference>
    <dbReference type="GeneID" id="60823367"/>
    <dbReference type="KEGG" id="rme:Rmet_4057"/>
    <dbReference type="eggNOG" id="COG2332">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_079503_1_1_4"/>
    <dbReference type="OMA" id="VTIHFRV"/>
    <dbReference type="OrthoDB" id="1724595at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017004">
        <property type="term" value="P:cytochrome complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017003">
        <property type="term" value="P:protein-heme linkage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01959">
        <property type="entry name" value="CcmE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004329">
        <property type="entry name" value="CcmE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036127">
        <property type="entry name" value="CcmE-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34128">
        <property type="entry name" value="PTHR34128"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03100">
        <property type="entry name" value="CcmE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82093">
        <property type="entry name" value="SSF82093"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="2 4" id="KW-0201">Cytochrome c-type biogenesis</keyword>
    <keyword evidence="1 4 5" id="KW-0349">Heme</keyword>
    <keyword evidence="4 5" id="KW-0408">Iron</keyword>
    <keyword evidence="3 4" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0614">Plasmid</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="topological domain" description="Cytoplasmic" evidence="4">
        <location>
            <begin position="1"/>
            <end position="7"/>
        </location>
    </feature>
    <feature type="topological domain" description="Periplasmic" evidence="4">
        <location>
            <begin position="29"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="128"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="binding site" description="covalent" evidence="4 5">
        <location>
            <position position="123"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="4 5">
        <location>
            <position position="127"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01959"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR604329-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABF10925.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="149" mass="16331" checksum="9221D9807546091E" modified="2006-05-30" version="1">MTRRQRRLGLLLAALLCGGTALAFVLNAFRSNLVFFFSPTQVAAHEAPASRNFRLGGLVAPGSLKRDPENLTIRFVVTDTRHDIPVVYRGLLPDLFREGKGVVARGALDRDGTFVASEILAKHDENYMPPEAADALKRAASDNPTEKTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LGD3</accession>
    <name>Q1LGD3_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Multidrug efflux system protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Rmet_3925</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="5">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="5">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the outer membrane factor (OMF) (TC 1.B.17) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10793.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518441.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3925"/>
    <dbReference type="EnsemblBacteria" id="ABF10793">
        <property type="protein sequence ID" value="ABF10793"/>
        <property type="gene ID" value="Rmet_3925"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3925"/>
    <dbReference type="eggNOG" id="COG1538">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012817_13_3_4"/>
    <dbReference type="OMA" id="AQYERTI"/>
    <dbReference type="OrthoDB" id="1619477at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015562">
        <property type="term" value="F:efflux transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003423">
        <property type="entry name" value="OMP_efflux"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010131">
        <property type="entry name" value="RND_efflux_OM_lipoprot_NodT"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02321">
        <property type="entry name" value="OEP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01845">
        <property type="entry name" value="outer_NodT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0564">Palmitate</keyword>
    <keyword evidence="4" id="KW-0614">Plasmid</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1134">Transmembrane beta strand</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5001442009" evidence="2">
        <location>
            <begin position="24"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="470"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="481"/>
            <end position="499"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU362097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF10793.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="499" mass="53917" checksum="003DA179768AE2FA" modified="2006-05-30" version="1">MRKTLLCLAAVAALSGCSLIPNYQRPAAPVDATFPQGEAYPKTAAVDASVTAVDLGWRDYFTDPRLRELIQVSLDNNRDLRVAVLNVEAYRAQYRIQRADLFPQVDAAAQGTRQRVPADLSATGQRVISSQYGVSLGTTAWELDLFGRLRSLSEAAFEQYLAGDEARRSTQIALVASVANAYLTLRADEEQLKVTRDTLTTYERTYNLTRRRFEEGIASRLELRQSETQVETARASLAAYTRALAQDTNALTLLVGSGMPADLPQGLGLEEKLVAEVPVGLPSDLLQNRPDILSAEHRLKAANADIGAARAAFFPRITLTASAGTASRQLSDLFAAGQGTWLFSPQIVLPIFTAGSLQASLDYAKIQKDINVAAYERTIQGAFREVADGLAARGTFSDQVQAQVRLVRASQDYFTLAEHRYSTGVDSYLTLLDAQRQLYNAQQTLITDRLNQLTSEVNLYKALGGGWTDVTHRPENGAPADGAGSSTTGSTGGGAGSAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LGT7</accession>
    <name>Q1LGT7_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Alkylated DNA repair protein alk</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">alkB1</name>
        <name evidence="7" type="ordered locus">Rmet_3769</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="8">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="8">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10639.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3769"/>
    <dbReference type="EnsemblBacteria" id="ABF10639">
        <property type="protein sequence ID" value="ABF10639"/>
        <property type="gene ID" value="Rmet_3769"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3769"/>
    <dbReference type="eggNOG" id="COG3145">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039677_1_1_4"/>
    <dbReference type="OMA" id="FRNMETP"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051213">
        <property type="term" value="F:dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.590">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004574">
        <property type="entry name" value="Alkb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027450">
        <property type="entry name" value="AlkB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037151">
        <property type="entry name" value="AlkB-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005123">
        <property type="entry name" value="Oxoglu/Fe-dep_dioxygenase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16557">
        <property type="entry name" value="PTHR16557"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13532">
        <property type="entry name" value="2OG-FeII_Oxy_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51471">
        <property type="entry name" value="FE2OG_OXY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="2" id="KW-0227">DNA damage</keyword>
    <keyword evidence="5" id="KW-0234">DNA repair</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-0614">Plasmid</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fe2OG dioxygenase" evidence="6">
        <location>
            <begin position="94"/>
            <end position="194"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABF10639.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="194" mass="21314" checksum="5F90DC2889ABFCC5" modified="2006-05-30" version="1">MVLRGFALPYVPDLMPAIAGIETTSPFRHMVTPGGFTMSVALTNCGALGWTTDRRGYRYTTVDPDTGKPWPVMPEVFFRLANEAAAEAGFDDFEPDACLLNRYLPGSRLALHQDKNEQAYETPIVSVSLGMRATFLFGGHARTAPTIKVPLHHGDVVVWGGADRLRYHGVMPIKDAPHALLGSQRINFTFRKAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LGY2</accession>
    <name>Q1LGY2_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">CMP/dCMP deaminase</fullName>
            <ecNumber evidence="4">3.5.4.3</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Rmet_3722</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="5">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="5">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EC" id="3.5.4.3" evidence="4"/>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10594.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518242.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3722"/>
    <dbReference type="EnsemblBacteria" id="ABF10594">
        <property type="protein sequence ID" value="ABF10594"/>
        <property type="gene ID" value="Rmet_3722"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3722"/>
    <dbReference type="eggNOG" id="COG0590">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025810_5_2_4"/>
    <dbReference type="OMA" id="CLGSLYY"/>
    <dbReference type="OrthoDB" id="1890883at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008892">
        <property type="term" value="F:guanine deaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016192">
        <property type="entry name" value="APOBEC/CMP_deaminase_Zn-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002125">
        <property type="entry name" value="CMP_dCMP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016193">
        <property type="entry name" value="Cytidine_deaminase-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00383">
        <property type="entry name" value="dCMP_cyt_deam_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53927">
        <property type="entry name" value="SSF53927"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00903">
        <property type="entry name" value="CYT_DCMP_DEAMINASES_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51747">
        <property type="entry name" value="CYT_DCMP_DEAMINASES_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0614">Plasmid</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="CMP/dCMP-type deaminase" evidence="3">
        <location>
            <begin position="4"/>
            <end position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF10594.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="156" mass="16743" checksum="6FD59E3D0D30A726" modified="2006-05-30" version="1">MNTNHAERYLGEAIELARTNLEQGGRPFGAVIVKDGEVIATGVNQILSTNDPTAHAELTAIRAASQKLGSPSLDGCAVYASGHPCPMCMAAMRMAGVKEVTYAYSNDDGEPYGLSTAAIYADLAKPFAEQSMKIRYMPVRPASCPDLYAEWKRRAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LH18</accession>
    <name>Q1LH18_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 5">Flagellar transcriptional regulator FlhC</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">flhC</name>
        <name evidence="6" type="synonym">flhC2</name>
        <name evidence="6" type="ordered locus">Rmet_3686</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="7">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="7">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Functions in complex with FlhD as a master transcriptional regulator that regulates transcription of several flagellar and non-flagellar operons by binding to their promoter region. Activates expression of class 2 flagellar genes, including fliA, which is a flagellum-specific sigma factor that turns on the class 3 genes. Also regulates genes whose products function in a variety of physiological pathways.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="4">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="4">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Heterohexamer composed of two FlhC and four FlhD subunits. Each FlhC binds a FlhD dimer, forming a heterotrimer, and a hexamer assembles by dimerization of two heterotrimers.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4 5">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 5">Belongs to the FlhC family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10558.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518208.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3686"/>
    <dbReference type="EnsemblBacteria" id="ABF10558">
        <property type="protein sequence ID" value="ABF10558"/>
        <property type="gene ID" value="Rmet_3686"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3686"/>
    <dbReference type="eggNOG" id="ENOG502Z927">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_122824_0_0_4"/>
    <dbReference type="OMA" id="MLQLSAC"/>
    <dbReference type="OrthoDB" id="1247512at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044781">
        <property type="term" value="P:bacterial-type flagellum organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045893">
        <property type="term" value="P:positive regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902208">
        <property type="term" value="P:regulation of bacterial-type flagellum assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01891">
        <property type="entry name" value="FhlC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007944">
        <property type="entry name" value="FlhC"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05280">
        <property type="entry name" value="FlhC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF003159">
        <property type="entry name" value="FlhC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0010">Activator</keyword>
    <keyword evidence="3 4 5" id="KW-1005">Bacterial flagellum biogenesis</keyword>
    <keyword evidence="1 4 5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 5 6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2 4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0614">Plasmid</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5" id="KW-0804">Transcription</keyword>
    <keyword evidence="4 5" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <feature type="binding site" evidence="4">
        <location>
            <position position="155"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="158"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="175"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="178"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022795"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01891"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRNR" id="PIRNR003159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABF10558.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="201" mass="22219" checksum="557E2F280DBF7B1C" modified="2006-05-30" version="1">MTALLQASPARSFAATPIPNRKSVLQDANQTQLAIELIGLGARLQVLEAETTLSRDRLIRLYKELRGASPPKGMLPFSTDWFTTWLPNIHSSLFFSAYQFMVQEGETSGIRAVVAAYRLYLEHVSLLGGEIVLSFTRAWTLVRFFESNMLQLSSCTCCGGQFVTHAYEPHTNFVCSLCRPPSRAGKVKKLSKDAAAAQTEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="56" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LH32</accession>
    <name>Q1LH32_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_3672</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10544.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518196.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF10544">
        <property type="protein sequence ID" value="ABF10544"/>
        <property type="gene ID" value="Rmet_3672"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3672"/>
    <dbReference type="HOGENOM" id="CLU_2737173_0_0_4"/>
    <dbReference type="OrthoDB" id="2065960at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF10544.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="69" mass="7550" checksum="2ACDF7ECEA3E76F0" modified="2006-05-30" version="1">MNARPDATAGTAAAEATKPVTRTRAGTAPFYYRLPDTPEGHHIKEYASGLRQLVRFVGVEEQVVKYSLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LH88</accession>
    <name>Q1LH88_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Osmotically inducible protein (OsmC-like)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_3616</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10488.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518147.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3616"/>
    <dbReference type="EnsemblBacteria" id="ABF10488">
        <property type="protein sequence ID" value="ABF10488"/>
        <property type="gene ID" value="Rmet_3616"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3616"/>
    <dbReference type="eggNOG" id="COG1765">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_100275_4_0_4"/>
    <dbReference type="OMA" id="MTIQATW"/>
    <dbReference type="OrthoDB" id="1430018at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015946">
        <property type="entry name" value="KH_dom-like_a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003718">
        <property type="entry name" value="OsmC/Ohr_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036102">
        <property type="entry name" value="OsmC/Ohrsf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02566">
        <property type="entry name" value="OsmC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82784">
        <property type="entry name" value="SSF82784"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF10488.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="129" mass="14054" checksum="397D75B83AFE777A" modified="2006-05-30" version="1">MTIEATWLANSGASLCDLSNGTAHWKADLDAPAGDATLPNPHDLLDSALAACTTLTLQIYAKRRNYDLQEVRVTVSHEEAPGTYKLVRRLELQGNLDEKAREDLLRVANKCPVHKVLSSQISIDTTLAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="100" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LHQ4</accession>
    <name>Q1LHQ4_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Tartronate semialdehyde reductase, NADH-dependent</fullName>
            <ecNumber evidence="6">1.1.1.60</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">glxR</name>
        <name evidence="6" type="ordered locus">Rmet_3450</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EC" id="1.1.1.60" evidence="6"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10322.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517881.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3450"/>
    <dbReference type="EnsemblBacteria" id="ABF10322">
        <property type="protein sequence ID" value="ABF10322"/>
        <property type="gene ID" value="Rmet_3450"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3450"/>
    <dbReference type="eggNOG" id="COG2084">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035117_1_0_4"/>
    <dbReference type="OMA" id="METPYPK"/>
    <dbReference type="OrthoDB" id="1194694at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008679">
        <property type="term" value="F:2-hydroxy-3-oxopropionate reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046487">
        <property type="term" value="P:glyoxylate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016054">
        <property type="term" value="P:organic acid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1040.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002204">
        <property type="entry name" value="3-OH-isobutyrate_DH-rel_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008927">
        <property type="entry name" value="6-PGluconate_DH-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013328">
        <property type="entry name" value="6PGD_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006115">
        <property type="entry name" value="6PGDH_NADP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015815">
        <property type="entry name" value="HIBADH-related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029154">
        <property type="entry name" value="NADP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006398">
        <property type="entry name" value="Tartro_sem_red"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14833">
        <property type="entry name" value="NAD_binding_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03446">
        <property type="entry name" value="NAD_binding_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000103">
        <property type="entry name" value="HIBADH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48179">
        <property type="entry name" value="SSF48179"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01505">
        <property type="entry name" value="tartro_sem_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00895">
        <property type="entry name" value="3_HYDROXYISOBUT_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0520">NAD</keyword>
    <keyword evidence="1 6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAD_binding_2" evidence="4">
        <location>
            <begin position="6"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="domain" description="NAD_binding_11" evidence="5">
        <location>
            <begin position="174"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="active site" evidence="3">
        <location>
            <position position="180"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR000103-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF03446"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF14833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABF10322.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="307" mass="31898" checksum="03D149A9A3B871F6" modified="2006-05-30" version="1">MANQRNVGFIGLGIMGAPMAGHLRAAGHTLFVHDVNPAPQFLVDAGVTVCTSAEEVAKRADIVIVMVPDTPHVEAVLFGEKGVAAAFKAAGKEASYSKIVVDMSSISPIATKDFAARINKLGASYLDAPVSGGEVGAKAATLTIMVGGPAEAFDQVKPLFELMGKNITLVGGNGDGQTTKVANQIIVALNIQAVSEALLFASKAGADPAKVRQALMGGFAASRILEVHGERMVKRTFDPGFRIELHQKDLNLALQGAKALGVSLPNTSTAQELFNACAANGFGKLDHSALCRAIEIMSNHEIAKGAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="60" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LHQ7</accession>
    <name>Q1LHQ7_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_3447</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10319.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517878.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3447"/>
    <dbReference type="EnsemblBacteria" id="ABF10319">
        <property type="protein sequence ID" value="ABF10319"/>
        <property type="gene ID" value="Rmet_3447"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3447"/>
    <dbReference type="HOGENOM" id="CLU_1007868_0_0_4"/>
    <dbReference type="OMA" id="ACYLYLH"/>
    <dbReference type="OrthoDB" id="1870996at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="32"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="65"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="151"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="177"/>
            <end position="196"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF10319.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="227" mass="24598" checksum="942DB077D13DE7D9" modified="2006-05-30" version="1">MYSRLPTTYELIEALQASPQAGPAAESRLATVLWAGFVLALVPMLVAGGALVWWHRVLAQVPPAWLPQVFHVLLAVCGVFYVGAWVIRLLDRRAALQHPLRWLTRRLDTQSEVENALLLRLGRIPALQLRARQRRVVLQARLWDASARTMALVLAIGPAAVILADGVAVMAPGGVPALVAIYGAVLVAGCALALFAQLQCSAPLRRLAHVLGEAAEINEAVNRQRQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-10-07" version="55" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LHZ6</accession>
    <name>Q1LHZ6_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_3358</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10230.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3358"/>
    <dbReference type="EnsemblBacteria" id="ABF10230">
        <property type="protein sequence ID" value="ABF10230"/>
        <property type="gene ID" value="Rmet_3358"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3358"/>
    <dbReference type="eggNOG" id="ENOG502Z7JB">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_097421_0_0_4"/>
    <dbReference type="OMA" id="SMTYRHA"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027802">
        <property type="entry name" value="Multi-ubiquitin_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14452">
        <property type="entry name" value="Multi_ubiq"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Multi_ubiq" evidence="1">
        <location>
            <begin position="2"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="domain" description="Multi_ubiq" evidence="1">
        <location>
            <begin position="69"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="domain" description="Multi_ubiq" evidence="1">
        <location>
            <begin position="141"/>
            <end position="212"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF14452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF10230.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="215" mass="23518" checksum="5A54394E228F116E" modified="2006-05-30" version="1">MNFIKIEIDDPVPLGRQVLTAAGMHGDDNYSLFNILESGDFEDVRLDEQIDLRRPGAERFIAFKSDRNFKLTVNGSQVVWGRPTISGADLYALSKPADGEAVFMVVSGGEDRQIEREDDVDLAAPGVERFENAPKRHPKVVIIVNGTKEELPAPLVTFDQLVALAYPGQPPQPGITYSITYYKVASYPHQGPMAPGGSVEAKNGSIFNVGRTIQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="62" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LI11</accession>
    <name>Q1LI11_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized membrane protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_3343</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10215.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517787.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3343"/>
    <dbReference type="EnsemblBacteria" id="ABF10215">
        <property type="protein sequence ID" value="ABF10215"/>
        <property type="gene ID" value="Rmet_3343"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3343"/>
    <dbReference type="eggNOG" id="COG0700">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG2715">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037414_0_0_4"/>
    <dbReference type="OMA" id="SNAQIMF"/>
    <dbReference type="OrthoDB" id="325457at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011642">
        <property type="entry name" value="Gate_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011415">
        <property type="entry name" value="SpmA_SpmB"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07670">
        <property type="entry name" value="Gate"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036542">
        <property type="entry name" value="SpmA_SpmB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="44"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="138"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="178"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="205"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="239"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="278"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="391"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="domain" description="Gate" evidence="2">
        <location>
            <begin position="51"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="domain" description="Gate" evidence="2">
        <location>
            <begin position="282"/>
            <end position="384"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF10215.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="414" mass="43987" checksum="787B4A66544B0B10" modified="2006-05-30" version="1">MALNLVWLGFFLVSFVAACVRLIGGDTAVFPAMLASLFDSARTAFEISLGLAGVMSLWIGIMRVGERAGAVDMFARLVNPLLRHFFPGVPQGHPAQGAMMMNISANMLGLDNAATPLGLNAMRELQSLNRQPSTATNAQIMFIVLNTAGLTLIPTSVIAIRQAMAVKQGLVGFNAADIFLPTLLATAVSCLAGLLAVAWVQRLNLLKPAVLVTFGLIGGLLGVLFFWLRHAPPDQVSKLTALAGSGFILSLITLFLVVGALRRINVYDAFIDGAKEGFGVAVQIIPYLVAILVAIGLFRVTGCMDVVMQALTSVFQWLGMNTDFVPALPVGLMKILSGAGARGLMVDVMTTYGVDSFQARLAAIIQGSTETTFYVLAVYFGSVNVRNTRHALACALFADGVGLVAAVWIAYLFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="106" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LI15</accession>
    <name>Q1LI15_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 6">Transcription termination/antitermination protein NusG</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5 9" type="primary">nusG</name>
        <name evidence="9" type="ordered locus">Rmet_3339</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5 6">Participates in transcription elongation, termination and antitermination.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5 6">Belongs to the NusG family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10211.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008649197.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3339"/>
    <dbReference type="EnsemblBacteria" id="ABF10211">
        <property type="protein sequence ID" value="ABF10211"/>
        <property type="gene ID" value="Rmet_3339"/>
    </dbReference>
    <dbReference type="GeneID" id="60826578"/>
    <dbReference type="KEGG" id="rme:Rmet_3339"/>
    <dbReference type="eggNOG" id="COG0250">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_067287_1_0_4"/>
    <dbReference type="OMA" id="DGPFNGF"/>
    <dbReference type="OrthoDB" id="2027986at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006354">
        <property type="term" value="P:DNA-templated transcription, elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006353">
        <property type="term" value="P:DNA-templated transcription, termination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032784">
        <property type="term" value="P:regulation of DNA-templated transcription, elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031564">
        <property type="term" value="P:transcription antitermination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.940">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00948">
        <property type="entry name" value="NusG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005824">
        <property type="entry name" value="KOW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006645">
        <property type="entry name" value="NGN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036735">
        <property type="entry name" value="NGN_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043425">
        <property type="entry name" value="NusG-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014722">
        <property type="entry name" value="Rib_L2_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001062">
        <property type="entry name" value="Transcrpt_antiterm_NusG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015869">
        <property type="entry name" value="Transcrpt_antiterm_NusG_bac_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008991">
        <property type="entry name" value="Translation_prot_SH3-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30265">
        <property type="entry name" value="PTHR30265"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30265:SF2">
        <property type="entry name" value="PTHR30265:SF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00467">
        <property type="entry name" value="KOW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02357">
        <property type="entry name" value="NusG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00338">
        <property type="entry name" value="NUSGTNSCPFCT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00739">
        <property type="entry name" value="KOW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00738">
        <property type="entry name" value="NGN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50104">
        <property type="entry name" value="SSF50104"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82679">
        <property type="entry name" value="SSF82679"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00922">
        <property type="entry name" value="nusG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01014">
        <property type="entry name" value="NUSG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5 6" id="KW-0804">Transcription</keyword>
    <keyword evidence="2 5 6" id="KW-0889">Transcription antitermination</keyword>
    <keyword evidence="3 5 6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="1 5 6" id="KW-0806">Transcription termination</keyword>
    <feature type="domain" description="NGN" evidence="7">
        <location>
            <begin position="18"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="domain" description="KOW" evidence="8">
        <location>
            <begin position="139"/>
            <end position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00948"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00739"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ABF10211.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="193" mass="22058" checksum="DC49EA83B866260D" modified="2006-05-30" version="1">MTDNAQQETTAAESPSSKKRWYVVHAYSGMEKSVQRALQERIERAEMQDKFGRILVPSEEVVEIKGGHKSVTERRFFPGYVLVEMEMTDETWHLVKNTSKVTGFVGGARNRPSPISQREVDKIMTQMQEGVEKPRPKTLFEVGEMVRVKDGPFTDFNGNVEEVNYEKSRLRVTVTIFGRATPVELEFGQVEKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LII4</accession>
    <name>Q1LII4_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Heme d1 biosynthesis protein NirJ</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">nirJ</name>
        <name evidence="7" type="ordered locus">Rmet_3170</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10042.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517654.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3170"/>
    <dbReference type="EnsemblBacteria" id="ABF10042">
        <property type="protein sequence ID" value="ABF10042"/>
        <property type="gene ID" value="Rmet_3170"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3170"/>
    <dbReference type="eggNOG" id="COG0535">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009273_4_0_4"/>
    <dbReference type="OMA" id="PVVIWNL"/>
    <dbReference type="OrthoDB" id="1172757at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaB/NifB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023992">
        <property type="entry name" value="HemeD1_Synth_NirJ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017200">
        <property type="entry name" value="PqqE-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037420">
        <property type="entry name" value="PQQ_syn_pqqE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00393">
        <property type="entry name" value="heme_D1_biosynthesis_(NirJ-lik"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR04051">
        <property type="entry name" value="rSAM_NirJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0408">Iron</keyword>
    <keyword evidence="5" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <feature type="domain" description="Radical SAM core" evidence="6">
        <location>
            <begin position="22"/>
            <end position="233"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABF10042.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="387" mass="42457" checksum="B4855C1028794A51" modified="2006-05-30" version="1">MFRLSRFMEALRDGGSVPPPRRASGPVVIWNLVRRCNLNCKHCYSTSADTDFKGELSTGEVMAVLDQLKAARVPALILSGGEPLLRPDLYEIAARARELGFHLSLSSNGTLLDAAHAARLAAAGFDYVGVSLDGIEATHDRFRRMNGAFTQAIAGLRIAREAGLRVGVRMTLTEANAAELPSVVALAEAEGIDKFYLSHFNYAGRGRSHRNDDARHAGTRVAVEWLFEHVWARAQQGSAGDFVTGNNDADGVFLLYWIARQWPERVADMRERLVNWGGNATGVGVANIDNLGNVHPDTMWWQLSLGNVRERPFGEIWADRSHPLMRGLAARPRPLQGRCATCLHRDICNGNTRARAYAVSGNPWAQDPGCYLSDAEISAQPNLETVT</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-01-15" modified="2022-05-25" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LIP9</accession>
    <name>RL27_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">50S ribosomal protein L27</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpmA</name>
        <name type="ordered locus">Rmet_3105</name>
    </gene>
    <organism>
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the bacterial ribosomal protein bL27 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09977.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008647258.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q1LIP9"/>
    <dbReference type="SMR" id="Q1LIP9"/>
    <dbReference type="STRING" id="266264.Rmet_3105"/>
    <dbReference type="EnsemblBacteria" id="ABF09977">
        <property type="protein sequence ID" value="ABF09977"/>
        <property type="gene ID" value="Rmet_3105"/>
    </dbReference>
    <dbReference type="GeneID" id="60820520"/>
    <dbReference type="KEGG" id="rme:Rmet_3105"/>
    <dbReference type="eggNOG" id="COG0211">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_095424_4_1_4"/>
    <dbReference type="OMA" id="GKDHTLH"/>
    <dbReference type="OrthoDB" id="1904463at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00539">
        <property type="entry name" value="Ribosomal_L27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001684">
        <property type="entry name" value="Ribosomal_L27"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018261">
        <property type="entry name" value="Ribosomal_L27_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15893">
        <property type="entry name" value="PTHR15893"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01016">
        <property type="entry name" value="Ribosomal_L27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00063">
        <property type="entry name" value="RIBOSOMALL27"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00062">
        <property type="entry name" value="L27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00831">
        <property type="entry name" value="RIBOSOMAL_L27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <feature type="chain" id="PRO_1000017572" description="50S ribosomal protein L27">
        <location>
            <begin position="1"/>
            <end position="86"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00539"/>
        </source>
    </evidence>
    <sequence length="86" mass="8877" checksum="AA4478DA91064121" modified="2006-05-30" version="1">MAQKKGGGSTRNGRDSESKRLGVKVFGGQAINAGGIIVRQRGTRVHAGENVGIGKDHTLFALVDGHVQFAVKGAAKKQQVSVVPAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="53" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LIY2</accession>
    <name>Q1LIY2_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_3021</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09894.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_003056183.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3021"/>
    <dbReference type="EnsemblBacteria" id="ABF09894">
        <property type="protein sequence ID" value="ABF09894"/>
        <property type="gene ID" value="Rmet_3021"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3021"/>
    <dbReference type="eggNOG" id="ENOG502Z7UG">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_123182_0_0_4"/>
    <dbReference type="OMA" id="RWIRFGH"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021263">
        <property type="entry name" value="DUF2840"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11000">
        <property type="entry name" value="DUF2840"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF09894.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="174" mass="19197" checksum="2513D07DDF06DBBC" modified="2006-05-30" version="1">MNTPASTASAPPSIPPVGVADGVPLTRVALAYIDQRFDLYLRFGDPARIIRFDRWRRCAVFTPNAVLCRIRWQANDYGTIRWQLMVMQACMPMDGAQRISGVQPGARLLLHAEGEQSVRAVLARIDAIEALGITPVGVSPAYWRTLANRLAAHLPLPEYTAERHAAWLAGRALP</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2007-10-02" modified="2022-08-03" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LJF1</accession>
    <name>HUTI_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Imidazolonepropionase</fullName>
            <ecNumber evidence="1">3.5.2.7</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Imidazolone-5-propionate hydrolase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">hutI</name>
        <name type="ordered locus">Rmet_2852</name>
    </gene>
    <organism>
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the hydrolytic cleavage of the carbon-nitrogen bond in imidazolone-5-propanoate to yield N-formimidoyl-L-glutamate. It is the third step in the universal histidine degradation pathway.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>4-imidazolone-5-propanoate + H2O = N-formimidoyl-L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:23660"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:58928"/>
            <dbReference type="ChEBI" id="CHEBI:77893"/>
            <dbReference type="EC" id="3.5.2.7"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <cofactor evidence="1">
            <name>Fe(3+)</name>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
        </cofactor>
        <text evidence="1">Binds 1 zinc or iron ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid degradation; L-histidine degradation into L-glutamate; N-formimidoyl-L-glutamate from L-histidine: step 3/3.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the metallo-dependent hydrolases superfamily. HutI family.</text>
    </comment>
    <dbReference type="EC" id="3.5.2.7" evidence="1"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09725.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517410.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q1LJF1"/>
    <dbReference type="SMR" id="Q1LJF1"/>
    <dbReference type="STRING" id="266264.Rmet_2852"/>
    <dbReference type="PRIDE" id="Q1LJF1"/>
    <dbReference type="EnsemblBacteria" id="ABF09725">
        <property type="protein sequence ID" value="ABF09725"/>
        <property type="gene ID" value="Rmet_2852"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2852"/>
    <dbReference type="eggNOG" id="COG1228">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041647_0_0_4"/>
    <dbReference type="OMA" id="CAPHARW"/>
    <dbReference type="OrthoDB" id="785930at2"/>
    <dbReference type="UniPathway" id="UPA00379">
        <property type="reaction ID" value="UER00551"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050480">
        <property type="term" value="F:imidazolonepropionase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019556">
        <property type="term" value="P:histidine catabolic process to glutamate and formamide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019557">
        <property type="term" value="P:histidine catabolic process to glutamate and formate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd01296">
        <property type="entry name" value="Imidazolone-5PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00372">
        <property type="entry name" value="HutI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006680">
        <property type="entry name" value="Amidohydro-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005920">
        <property type="entry name" value="HutI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011059">
        <property type="entry name" value="Metal-dep_hydrolase_composite"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42752">
        <property type="entry name" value="PTHR42752"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01979">
        <property type="entry name" value="Amidohydro_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51338">
        <property type="entry name" value="SSF51338"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="SSF51556"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01224">
        <property type="entry name" value="hutI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0369">Histidine metabolism</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0408">Iron</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <feature type="chain" id="PRO_0000306496" description="Imidazolonepropionase">
        <location>
            <begin position="1"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="74"/>
        </location>
        <ligand>
            <name>Fe(3+)</name>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="74"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="76"/>
        </location>
        <ligand>
            <name>Fe(3+)</name>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="76"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="83"/>
        </location>
        <ligand>
            <name>4-imidazolone-5-propanoate</name>
            <dbReference type="ChEBI" id="CHEBI:77893"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="146"/>
        </location>
        <ligand>
            <name>4-imidazolone-5-propanoate</name>
            <dbReference type="ChEBI" id="CHEBI:77893"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="146"/>
        </location>
        <ligand>
            <name>N-formimidoyl-L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:58928"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="179"/>
        </location>
        <ligand>
            <name>4-imidazolone-5-propanoate</name>
            <dbReference type="ChEBI" id="CHEBI:77893"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="244"/>
        </location>
        <ligand>
            <name>Fe(3+)</name>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="244"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="247"/>
        </location>
        <ligand>
            <name>4-imidazolone-5-propanoate</name>
            <dbReference type="ChEBI" id="CHEBI:77893"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="319"/>
        </location>
        <ligand>
            <name>Fe(3+)</name>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="319"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="321"/>
        </location>
        <ligand>
            <name>N-formimidoyl-L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:58928"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="323"/>
        </location>
        <ligand>
            <name>N-formimidoyl-L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:58928"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="324"/>
        </location>
        <ligand>
            <name>4-imidazolone-5-propanoate</name>
            <dbReference type="ChEBI" id="CHEBI:77893"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00372"/>
        </source>
    </evidence>
    <sequence length="415" mass="44303" checksum="2FA81F7A77116921" modified="2006-05-30" version="1">MKFNEAPSADGVWHHCHLLPAADPAQAIRDGAIVVEQGRIAWLGAFAALPDVYRHLPRHDANGAWITPGLVDCHTHLVYGGQRADEFAMRLAGASYEEIARAGGGIVSTVRATREADEATLFAQASARLEPLLAEGVTAIEIKSGYGLSLEAERKQLRVARQLGETYGVAVHTTFLGAHALPPEYAGRADAYIELVCETMLPALAAEGLVDAVDAFCEGIGFSIAQTTRVFEAAARHGLPVKLHAEQLSNLGGAALAARHKAISADHLEHLDEAGVVAMAEAGTVAVLLPGAYYFLRDTQLPPLALLRKHGVPMAISTDHNPGTSPTTSLLLMMNMGCTIFRMTVPEVLAGVTTHAARALGAADRHGLLAVDRAADFVLWQVASPAELAYWFGRNPCAAVVRRGRVFRKGEGWQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="64" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LJL1</accession>
    <name>Q1LJL1_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">EVE domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_2792</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09665.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008644085.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2792"/>
    <dbReference type="EnsemblBacteria" id="ABF09665">
        <property type="protein sequence ID" value="ABF09665"/>
        <property type="gene ID" value="Rmet_2792"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2792"/>
    <dbReference type="eggNOG" id="COG2947">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041799_2_2_4"/>
    <dbReference type="OMA" id="LWDGIRN"/>
    <dbReference type="OrthoDB" id="1829674at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002740">
        <property type="entry name" value="EVE_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015947">
        <property type="entry name" value="PUA-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01878">
        <property type="entry name" value="EVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88697">
        <property type="entry name" value="SSF88697"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="EVE" evidence="1">
        <location>
            <begin position="10"/>
            <end position="155"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF09665.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="159" mass="18202" checksum="DD5E96EF97A32627" modified="2006-05-30" version="1">MPSSAQRARQYWLMKSEPDEASIDDLAREGTLPWTGVRNYQARNFMRDQMRIGDGVLFYHSSCPEPGIAGLAEVCSQPYPDSTQFDPKSDYYDSASKQDNPRWSLVDVSFVRKSTLIPLQALREHDALADMVVLRRGNRLSITPVTPAEWRYITEKLMK</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="121" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LJM5</accession>
    <name>Q1LJM5_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 7">Cobyric acid synthase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7 10" type="primary">cobQ</name>
        <name evidence="10" type="ordered locus">Rmet_2778</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6 7">Catalyzes amidations at positions B, D, E, and G on adenosylcobyrinic A,C-diamide. NH(2) groups are provided by glutamine, and one molecule of ATP is hydrogenolyzed for each amidation.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1 7">Cofactor biosynthesis; adenosylcobalamin biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the CobB/CobQ family. CobQ subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09651.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2778"/>
    <dbReference type="EnsemblBacteria" id="ABF09651">
        <property type="protein sequence ID" value="ABF09651"/>
        <property type="gene ID" value="Rmet_2778"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2778"/>
    <dbReference type="eggNOG" id="COG1492">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019250_2_2_4"/>
    <dbReference type="OMA" id="EIHHGVA"/>
    <dbReference type="UniPathway" id="UPA00148"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015420">
        <property type="term" value="F:ABC-type vitamin B12 transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016874">
        <property type="term" value="F:ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009236">
        <property type="term" value="P:cobalamin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006541">
        <property type="term" value="P:glutamine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01750">
        <property type="entry name" value="GATase1_CobQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00028">
        <property type="entry name" value="CobQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002586">
        <property type="entry name" value="CobQ/CobB/MinD/ParA_Nub-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033949">
        <property type="entry name" value="CobQ_GATase1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004459">
        <property type="entry name" value="CobQ_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011698">
        <property type="entry name" value="GATase_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21343:SF1">
        <property type="entry name" value="PTHR21343:SF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01656">
        <property type="entry name" value="CbiA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07685">
        <property type="entry name" value="GATase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="SSF52317"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00313">
        <property type="entry name" value="cobQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0169">Cobalamin biosynthesis</keyword>
    <keyword evidence="5 7" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="10" id="KW-0436">Ligase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CbiA" evidence="8">
        <location>
            <begin position="22"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="domain" description="GATase_3" evidence="9">
        <location>
            <begin position="271"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="7">
        <location>
            <position position="350"/>
        </location>
    </feature>
    <feature type="active site" evidence="7">
        <location>
            <position position="449"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00019833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022573"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00025166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF07685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ABF09651.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="505" mass="53440" checksum="BED975F51A492CEB" modified="2006-05-30" version="1">MRDEPASRTDFLPAGRALHGTLMIQGTTSDAGKSTVVAGLCRVLARAGVPVAPFKPQNMALNSAVTVDGGEIGRAQALQAQAAGLAPHTDMNPVLLKPSSDTGAQVIIHGRARMDLDARAYHDYKPRAMAAVLESHERLAQAYDVVLVEGAGSPAEVNLRDRDIANMGFAERVDCPVVIVADIDRGGVFAHLVGTLDCLSETERTRVTGFIINRFRGDIALLEPGLDWLTARTGKPVFGVLPYLHGLHLDAEDAIVSAQVAGQAGGSEVLRVIVPVLPRISNHTDFDALRAHPGVDLQFIGPGMPIPPADLVILPGSKSVRADLAFLRANGWDRALRRHLRYGGKLIGICGGMQMLGRSIHDPDGREGPAGTSDGLALLDFETTLAPEKQLRRVAGRLADGNDATVRGYEIHLGVTEGPALARPALWLEGDRPDGARSDDGQVLATYVHGLFDDPAACGALLRWAGLGQAEGVDLDALREASLDRLADSLAANLDLGALFAPLGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="56" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LJR6</accession>
    <name>Q1LJR6_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_2737</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09610.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2737"/>
    <dbReference type="EnsemblBacteria" id="ABF09610">
        <property type="protein sequence ID" value="ABF09610"/>
        <property type="gene ID" value="Rmet_2737"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2737"/>
    <dbReference type="eggNOG" id="COG3765">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_715467_0_0_4"/>
    <dbReference type="OrthoDB" id="1547209at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="31"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="353"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="173"/>
            <end position="214"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF09610.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="398" mass="44236" checksum="AC394C2E06806DFD" modified="2006-05-30" version="1">MGQKEEFGQAVNWSPVAKSIVRRISANRRPFLLTTALASLALFALMSYLLRHYQSEGFLRASRPLTEFNAQRATFEDKESFQRFLRANKLMDSPNAVYLAHAIGPGLMQRQVKVVLPYTKDDLRFLTDLKTPLDSNILGFSVTFAGATPDDAAARVSLMGDYLKDTMLRQDLLDNIHQRAGEVRAEKQKLDNKLIAKRLELEEAARKLSALKEIAAKYPEASKYESRQLLSSDTDGARYLSPTMQLVGVESSIVDIRNELASLERDEAQNDLRLKFYAGAEKLNPSVKSGSELFEGFKALRAETFKDVRLDDDRTREVVNKIDLLAETLQTKHLMNTRFTSGPSLPDHRSGPAIGLMLLLSVVLGGTFAAMAIAVLGWVQAKNGKRAISGLADPGMQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LK10</accession>
    <name>Q1LK10_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Putative dinucleotide-utilizing enzyme involved in molybdopterin and thiamine biosynthesis</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Rmet_2639</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09516.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2639"/>
    <dbReference type="EnsemblBacteria" id="ABF09516">
        <property type="protein sequence ID" value="ABF09516"/>
        <property type="gene ID" value="Rmet_2639"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2639"/>
    <dbReference type="eggNOG" id="COG1179">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013325_4_0_4"/>
    <dbReference type="OMA" id="DMDDICV"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008641">
        <property type="term" value="F:ubiquitin-like modifier activating enzyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045886">
        <property type="entry name" value="ThiF/MoeB/HesA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000594">
        <property type="entry name" value="ThiF_NAD_FAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035985">
        <property type="entry name" value="Ubiquitin-activating_enz"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43267">
        <property type="entry name" value="PTHR43267"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00899">
        <property type="entry name" value="ThiF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69572">
        <property type="entry name" value="SSF69572"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="276"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="domain" description="ThiF" evidence="3">
        <location>
            <begin position="54"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="8"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF09516.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="302" mass="32370" checksum="BF5B91DCC6CFFD5D" modified="2006-05-30" version="1">MPARFVGSRCRQTQQQERMTLTPTPLAAQDLPAPLSHESPEDDDYHRRFGGVARLYGAEGLARLETANVCVIGIGGVGSWAAEALARNAVGRLTLIDLDHIAASNTNRQIHALGDAYGRSKVDAMAERIVAINPRCRVTAVDDFVTEDNVQALLSGFDYVIDAIDAVKVKTAILGWARREDARVITCGGAGGQLDPTRVRIEDLARTIQDPLLAKVRGNLRRQWGFPRDPKKKFGIQAVYSDEPLRYPEPEQQACEIDEVPPAPRQGPQGLACAGFGSSVAVTAVFGFVAASAVIGAIASGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LKG9</accession>
    <name>Q1LKG9_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Leucine/isoleucine/valine transporter subunit, periplasmic-binding component of ABC superfamily</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">livK1</name>
        <name evidence="5" type="ordered locus">Rmet_2480</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the leucine-binding protein family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09357.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008649366.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2480"/>
    <dbReference type="EnsemblBacteria" id="ABF09357">
        <property type="protein sequence ID" value="ABF09357"/>
        <property type="gene ID" value="Rmet_2480"/>
    </dbReference>
    <dbReference type="GeneID" id="60821045"/>
    <dbReference type="KEGG" id="rme:Rmet_2480"/>
    <dbReference type="eggNOG" id="COG0683">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027128_6_0_4"/>
    <dbReference type="OMA" id="MEFTGAR"/>
    <dbReference type="OrthoDB" id="776281at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006865">
        <property type="term" value="P:amino acid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028081">
        <property type="entry name" value="Leu-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000709">
        <property type="entry name" value="Leu_Ile_Val-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028082">
        <property type="entry name" value="Peripla_BP_I"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13458">
        <property type="entry name" value="Peripla_BP_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00337">
        <property type="entry name" value="LEUILEVALBP"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53822">
        <property type="entry name" value="SSF53822"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0029">Amino-acid transport</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193580" evidence="3">
        <location>
            <begin position="29"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="domain" description="Peripla_BP_6" evidence="4">
        <location>
            <begin position="30"/>
            <end position="368"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010062"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF13458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABF09357.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="375" mass="39315" checksum="15765D30B9136EA1" modified="2006-05-30" version="1">MTLSRLTSTAMAAVLATAGLAAFTSANAETIKIAIAGPMSGSVAQYGDMVKAGALTAIEQINAAGGANGNKFEAVMMDDACEPKQAVAVANKIVSQGIHYVIGHVCSGSTIPASDIYENEGIVMVTPSATAPQLTETKKRNFIFRTIGRDDQQGPAAAQYIINKVKPKKVAVLHDKQSYGQGIASSVKKDLEAAKIPVAVFEGINAGDSDYSAVITKLKSQGVDFVYFGGYHPEMGLLLRQAREQGVKATFMGPEGVGNKDVTAIAGPASEGMLVTLPADFSADPANAALVKAFADKKRDPNGPFQMPAYAAVKIIGDAITGAKSTDPTKVAAYMHKNAFNTPIGKVEYDAKGDLKSFKFVVYTWHKDATKTAAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="110" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LKI0</accession>
    <name>Q1LKI0_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">tRNA pseudouridine synthase A</fullName>
            <ecNumber evidence="4">5.4.99.12</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">tRNA pseudouridine(38-40) synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="4">tRNA pseudouridylate synthase I</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="4">tRNA-uridine isomerase I</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="4 9" type="primary">truA</name>
        <name evidence="9" type="ordered locus">Rmet_2469</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4 7">
            <text>uridine(38/39/40) in tRNA = pseudouridine(38/39/40) in tRNA</text>
            <dbReference type="Rhea" id="RHEA:22376"/>
            <dbReference type="Rhea" id="RHEA-COMP:10085"/>
            <dbReference type="Rhea" id="RHEA-COMP:10087"/>
            <dbReference type="ChEBI" id="CHEBI:65314"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="EC" id="5.4.99.12"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4 7">Belongs to the tRNA pseudouridine synthase TruA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="5.4.99.12" evidence="4"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09346.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2469"/>
    <dbReference type="EnsemblBacteria" id="ABF09346">
        <property type="protein sequence ID" value="ABF09346"/>
        <property type="gene ID" value="Rmet_2469"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2469"/>
    <dbReference type="eggNOG" id="COG0101">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014673_0_2_4"/>
    <dbReference type="OMA" id="FLYGMVR"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106029">
        <property type="term" value="F:tRNA pseudouridine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031119">
        <property type="term" value="P:tRNA pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02570">
        <property type="entry name" value="PseudoU_synth_EcTruA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.580">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.660">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00171">
        <property type="entry name" value="TruA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020103">
        <property type="entry name" value="PsdUridine_synth_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001406">
        <property type="entry name" value="PsdUridine_synth_TruA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020097">
        <property type="entry name" value="PsdUridine_synth_TruA_a/b_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020095">
        <property type="entry name" value="PsdUridine_synth_TruA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020094">
        <property type="entry name" value="TruA/RsuA/RluB/E/F_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11142">
        <property type="entry name" value="PTHR11142"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01416">
        <property type="entry name" value="PseudoU_synth_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001430">
        <property type="entry name" value="tRNA_psdUrid_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55120">
        <property type="entry name" value="SSF55120"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00071">
        <property type="entry name" value="hisT_truA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4 7" id="KW-0413">Isomerase</keyword>
    <keyword evidence="9" id="KW-0456">Lyase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4 7" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="PseudoU_synth_1" evidence="8">
        <location>
            <begin position="11"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="domain" description="PseudoU_synth_1" evidence="8">
        <location>
            <begin position="150"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="4 5">
        <location>
            <position position="54"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4 6">
        <location>
            <position position="112"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR001430-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR001430-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01416"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ABF09346.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="278" mass="31365" checksum="1D6B1F1CEBA9C55E" modified="2006-05-30" version="1">MPVNRIAIGLHYDGSAYSGWQSQPHRNTVQDHLEDAIERFAGVRLLTTVAGRTDAGVHALGQVIHLDTELDRDRFSWVRGVNAFLPPSIALQWAEPVDENFHARFLAYERMYYYALYTGPHRAPMIHGRAGYLMLPHGQRLDTEAMRRASACLLGEHDFSAFRAAECQAKSPVKTMYDVTIEADGNWVFLRFRASAFLHHMVRNLMGCLVAVGRGRYPAEWMAEVLEGRNRQKAAPTFMPDGLYLVDVKYPEPYQLPKADPAASFFHGVFVDDSHDGA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-03-03" modified="2022-08-03" version="95" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LKJ7</accession>
    <name>FENR2_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Ferredoxin--NADP reductase 2</fullName>
            <shortName evidence="1">FNR 2</shortName>
            <shortName evidence="1">Fd-NADP(+) reductase 2</shortName>
            <ecNumber evidence="1">1.18.1.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">Rmet_2452</name>
    </gene>
    <organism>
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>H(+) + NADP(+) + 2 reduced [2Fe-2S]-[ferredoxin] = NADPH + 2 oxidized [2Fe-2S]-[ferredoxin]</text>
            <dbReference type="Rhea" id="RHEA:20125"/>
            <dbReference type="Rhea" id="RHEA-COMP:10000"/>
            <dbReference type="Rhea" id="RHEA-COMP:10001"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33737"/>
            <dbReference type="ChEBI" id="CHEBI:33738"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.18.1.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
        <text evidence="1">Binds 1 FAD per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the ferredoxin--NADP reductase type 2 family.</text>
    </comment>
    <dbReference type="EC" id="1.18.1.2" evidence="1"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09329.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008648409.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q1LKJ7"/>
    <dbReference type="SMR" id="Q1LKJ7"/>
    <dbReference type="STRING" id="266264.Rmet_2452"/>
    <dbReference type="EnsemblBacteria" id="ABF09329">
        <property type="protein sequence ID" value="ABF09329"/>
        <property type="gene ID" value="Rmet_2452"/>
    </dbReference>
    <dbReference type="GeneID" id="60821074"/>
    <dbReference type="KEGG" id="rme:Rmet_2452"/>
    <dbReference type="eggNOG" id="COG0492">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031864_5_5_4"/>
    <dbReference type="OMA" id="PEKVIYD"/>
    <dbReference type="OrthoDB" id="692968at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004324">
        <property type="term" value="F:ferredoxin-NADP+ reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01685">
        <property type="entry name" value="FENR2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022890">
        <property type="entry name" value="Fd--NADP_Rdtase_type_2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="SSF51905"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0274">FAD</keyword>
    <keyword id="KW-0285">Flavoprotein</keyword>
    <keyword id="KW-0521">NADP</keyword>
    <keyword id="KW-0560">Oxidoreductase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000364908" description="Ferredoxin--NADP reductase 2">
        <location>
            <begin position="1"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="56"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="64"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="69"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="109"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="144"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="309"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="350"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01685"/>
        </source>
    </evidence>
    <sequence length="367" mass="40152" checksum="31884C147AF1D894" modified="2006-05-30" version="1">MDLSIPNPVADATHQAGGNPGGQPLEIDALIVGAGPVGLFQVFELGLLEIKAHIIDSLKVVGGQCVELYPDKPIYDIPAVPICTGQELTDNLLKQIEPFSPTFHLGQEVSVVERREDGRFFVETSLGTRFITKTIFIAAGVGSFQPRTLKVEGIDKFEGKQLFYRVKDPSRFHGRNLVVVGGGDSALDWTLDLVGKAESVVMIHRRDGFRAAPASVAKMRELCEQMEMQFMVGQIGGYEEKDGVLTEIKVTGADGVTRRMPVDDILVFFGLSPKLGPIAEWGLDLERKQIKVDTEKFETNIPGIFAVGDINTYPGKKKLILSGFHEAALAAFGAAPYIFPDKKIHMQYTTTSPKLHKVLGVETPVFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="65" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LKX8</accession>
    <name>Q1LKX8_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">yubP</name>
        <name evidence="1" type="ordered locus">Rmet_2321</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09198.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_003116824.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2321"/>
    <dbReference type="EnsemblBacteria" id="ABF09198">
        <property type="protein sequence ID" value="ABF09198"/>
        <property type="gene ID" value="Rmet_2321"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2321"/>
    <dbReference type="eggNOG" id="COG1961">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_059317_0_0_4"/>
    <dbReference type="OMA" id="RFGEAHQ"/>
    <dbReference type="OrthoDB" id="716673at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026325">
        <property type="entry name" value="DUF932"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06067">
        <property type="entry name" value="DUF932"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF09198.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="275" mass="30998" checksum="FB37E96F048E5A08" modified="2006-05-30" version="1">MSLVSRFAPQSPILRSDRPLSDDRIRAVVPSIFADAPHGSRSDRYAYIPTSTVLTKLRQEGFEPFMVCQTRVRNEDRREYTKHLIRLRHASQINGDEANEIILLNSHDGTSSYQMLAGMFRFVCHNGLVCGDTTADIRVPHKGDVASQVIEGAYGVLEGFERVHNARDAMRTITLDEGEAEVFANSALALKYDDPAKSTPVTESQLLAPRRWDDRKNDLWAVFNRVQENLVKGGLNGRTANGRNQRTRPVQGIDQNLRLNRALWLLAEGMRQLKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="70" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LKY4</accession>
    <name>Q1LKY4_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Mercuric transport protein MerT</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Mercury ion transport protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="primary">merT</name>
        <name evidence="16" type="ordered locus">Rmet_2313</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell inner membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the MerT family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09192.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_003116827.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2313"/>
    <dbReference type="EnsemblBacteria" id="ABF09192">
        <property type="protein sequence ID" value="ABF09192"/>
        <property type="gene ID" value="Rmet_2313"/>
    </dbReference>
    <dbReference type="GeneID" id="61526439"/>
    <dbReference type="KEGG" id="rme:Rmet_2313"/>
    <dbReference type="eggNOG" id="COG2608">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_151729_0_0_4"/>
    <dbReference type="OMA" id="MEPERVN"/>
    <dbReference type="OrthoDB" id="1896057at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015097">
        <property type="term" value="F:mercury ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003457">
        <property type="entry name" value="Transprt_MerT"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02411">
        <property type="entry name" value="MerT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="7" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="13 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0475">Mercuric resistance</keyword>
    <keyword evidence="11" id="KW-0476">Mercury</keyword>
    <keyword evidence="10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12 15" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="12"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="53"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="90"/>
            <end position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004429"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00017053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00030934"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="ABF09192.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="116" mass="12226" checksum="C955EE580C988827" modified="2006-05-30" version="1">MSEPKNGCGALATGGVAAVLASACCLGPLVLVALGFSGAWIGNLTVLEPYRPIFIGAALIALFFAWRSIFRPAHACKPGDVCAVPQVRTAYKVIFWIVAALVLVALAFPYVLPLFY</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-10-07" version="72" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LL26</accession>
    <name>Q1LL26_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_2271</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09150.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2271"/>
    <dbReference type="EnsemblBacteria" id="ABF09150">
        <property type="protein sequence ID" value="ABF09150"/>
        <property type="gene ID" value="Rmet_2271"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2271"/>
    <dbReference type="eggNOG" id="COG0697">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033863_4_3_4"/>
    <dbReference type="OMA" id="LWMTGLK"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000620">
        <property type="entry name" value="EamA_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00892">
        <property type="entry name" value="EamA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="32"/>
            <end position="51"/>
        </location>
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        <location>
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        <location>
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            <end position="293"/>
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    <feature type="domain" description="EamA" evidence="2">
        <location>
            <begin position="5"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="domain" description="EamA" evidence="2">
        <location>
            <begin position="156"/>
            <end position="292"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF09150.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="308" mass="32634" checksum="37CCEF1E3E2F435C" modified="2006-05-30" version="1">MPIHAFFLIAAMALVGSNVGLGKSIIAEVPVLLFALLRFLIAIVCLSPWYRPARMRQVSRGEWINLFLMAFFGTFMFTLLMLGGVRLTSAMAAGVITSTIPATVAILSWLWLRERLSRRTVFSVLLAVAGIAVLNIARGSGHDAATSSGGGSGALIGNLMILGAVVCESIYVILSRRLSQTLAAIEICAYTHLIGGALMLPLGLVPLLTFDFGSVSGTTWAMLLWYALSASVFSFWLWMKGVRHVPAQLAGVFTSVLPVAAALYGIVVLGERPGWAHGVALACVLAGIVLASWPGPIARRALGGDPGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LL41</accession>
    <name>Q1LL41_CUPMC</name>
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        <recommendedName>
            <fullName evidence="10">Cellulose synthase catalytic subunit [UDP-forming]</fullName>
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        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
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        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
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            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
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    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
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                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
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                <person name="Vallaeys T."/>
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                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
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            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
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    </reference>
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        <text evidence="10">Catalytic subunit of cellulose synthase. It polymerizes uridine 5'-diphosphate glucose to cellulose.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 10">
            <text>[(1-&gt;4)-beta-D-glucosyl](n) + UDP-alpha-D-glucose = [(1-&gt;4)-beta-D-glucosyl](n+1) + H(+) + UDP</text>
            <dbReference type="Rhea" id="RHEA:19929"/>
            <dbReference type="Rhea" id="RHEA-COMP:10033"/>
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            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:18246"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
            <dbReference type="EC" id="2.4.1.12"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="10">Glycan metabolism; bacterial cellulose biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell inner membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
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    </dbReference>
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    </dbReference>
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    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="7 10" id="KW-0135">Cellulose biosynthesis</keyword>
    <keyword evidence="4 10 13" id="KW-0328">Glycosyltransferase</keyword>
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        </location>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004429"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU365020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF07238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ABF09135.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="857" mass="97095" checksum="B5FAE73D56F8D2C4" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-10-07" version="70" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LLD8</accession>
    <name>Q1LLD8_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative DNA-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_2159</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
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        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09038.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2159"/>
    <dbReference type="EnsemblBacteria" id="ABF09038">
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        <property type="gene ID" value="Rmet_2159"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2159"/>
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        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1377163_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00093">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.40">
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Cro/C1-type_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010982">
        <property type="entry name" value="Lambda_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01381">
        <property type="entry name" value="HTH_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00530">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47413">
        <property type="entry name" value="SSF47413"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50943">
        <property type="entry name" value="HTH_CROC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH cro/C1-type" evidence="2">
        <location>
            <begin position="93"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="163"/>
            <end position="198"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF09038.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="198" mass="22483" checksum="EB62674D71CC72CE" modified="2006-05-30" version="1">MPFLGCRHLHHFAWTPASRRGWIRARTTSELRRRQLAHPDALSQGYELPRPEFARTEWSGAADLSMGRWSPVQVMTRSHLTLDEMEAEIGGYLRTYRIHRNIDQATLSMRAGISVRALRNLETGNGSSLRTLCTVLRALGREGWLDMIAPVPTINPLMLTRQAKPRQRASKPRKKKTTFKEALAAIPNVGEDDSDSRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-10-07" version="52" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LLT4</accession>
    <name>Q1LLT4_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_2013</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08892.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2013"/>
    <dbReference type="EnsemblBacteria" id="ABF08892">
        <property type="protein sequence ID" value="ABF08892"/>
        <property type="gene ID" value="Rmet_2013"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2013"/>
    <dbReference type="HOGENOM" id="CLU_2357665_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="44"/>
            <end position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF08892.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="96" mass="10966" checksum="EACD27DD7682C05A" modified="2006-05-30" version="1">MFEHMSAEQTAEVIHQCIDMRLKGPFQQVAEAFKARDAKRTEDMAALRQELAALRGEVRALKAERRRADALGAEAERCELVRREAQAAESRLSRRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="126" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LLY8</accession>
    <name>Q1LLY8_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 13">Chaperone protein ClpB</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13 15" type="primary">clpB</name>
        <name evidence="15" type="ordered locus">Rmet_1959</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Part of a stress-induced multi-chaperone system, it is involved in the recovery of the cell from heat-induced damage, in cooperation with DnaK, DnaJ and GrpE. Acts before DnaK, in the processing of protein aggregates. Protein binding stimulates the ATPase activity; ATP hydrolysis unfolds the denatured protein aggregates, which probably helps expose new hydrophobic binding sites on the surface of ClpB-bound aggregates, contributing to the solubilization and refolding of denatured protein aggregates by DnaK.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Homohexamer. The oligomerization is ATP-dependent.</text>
    </comment>
    <comment type="subunit">
        <text evidence="13">Homohexamer; The oligomerization is ATP-dependent.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 13">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the ClpA/ClpB family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08838.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516684.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1959"/>
    <dbReference type="EnsemblBacteria" id="ABF08838">
        <property type="protein sequence ID" value="ABF08838"/>
        <property type="gene ID" value="Rmet_1959"/>
    </dbReference>
    <dbReference type="GeneID" id="60821435"/>
    <dbReference type="KEGG" id="rme:Rmet_1959"/>
    <dbReference type="eggNOG" id="COG0542">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005070_4_0_4"/>
    <dbReference type="OMA" id="SKMMQGE"/>
    <dbReference type="OrthoDB" id="44062at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008233">
        <property type="term" value="F:peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042026">
        <property type="term" value="P:protein refolding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009408">
        <property type="term" value="P:response to heat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1780.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017730">
        <property type="entry name" value="Chaperonin_ClpB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019489">
        <property type="entry name" value="Clp_ATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036628">
        <property type="entry name" value="Clp_N_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004176">
        <property type="entry name" value="Clp_R_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001270">
        <property type="entry name" value="ClpA/B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018368">
        <property type="entry name" value="ClpA/B_CS1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028299">
        <property type="entry name" value="ClpA/B_CS2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041546">
        <property type="entry name" value="ClpA/ClpB_AAA_lid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07724">
        <property type="entry name" value="AAA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17871">
        <property type="entry name" value="AAA_lid_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02861">
        <property type="entry name" value="Clp_N"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10431">
        <property type="entry name" value="ClpB_D2-small"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00300">
        <property type="entry name" value="CLPPROTEASEA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01086">
        <property type="entry name" value="ClpB_D2-small"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81923">
        <property type="entry name" value="SSF81923"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03346">
        <property type="entry name" value="chaperone_ClpB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51903">
        <property type="entry name" value="CLP_R"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00870">
        <property type="entry name" value="CLPAB_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00871">
        <property type="entry name" value="CLPAB_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 12 15" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 12" id="KW-0143">Chaperone</keyword>
    <keyword evidence="7 13" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="13" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="15" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-0645">Protease</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0677">Repeat</keyword>
    <keyword evidence="13" id="KW-0346">Stress response</keyword>
    <feature type="domain" description="Clp R" evidence="14">
        <location>
            <begin position="3"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="13">
        <location>
            <begin position="411"/>
            <end position="491"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008675"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00017574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00025613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00026057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU004432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU362034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51903"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="ABF08838.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="862" mass="95943" checksum="06679138DC5D5D09" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="95" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LM23</accession>
    <name>Q1LM23_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Short-chain dehydrogenase/reductase SDR</fullName>
            <ecNumber evidence="1">1.1.1.100</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_1924</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EC" id="1.1.1.100" evidence="1"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08803.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516655.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1924"/>
    <dbReference type="EnsemblBacteria" id="ABF08803">
        <property type="protein sequence ID" value="ABF08803"/>
        <property type="gene ID" value="Rmet_1924"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1924"/>
    <dbReference type="eggNOG" id="COG1028">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010194_1_2_4"/>
    <dbReference type="OMA" id="IRCNSVM"/>
    <dbReference type="OrthoDB" id="1601931at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004316">
        <property type="term" value="F:3-oxoacyl-[acyl-carrier-protein] reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF08803.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="238" mass="24796" checksum="2FCB7CA8159E4C6E" modified="2006-05-30" version="1">MAERRTALVTGATKGIGLALSKHLQQLGWSVIGVARHAIEGFPGQLLTADLADIQQTDAVLAQVVAASRIDAVINNAGIALPQKLESLDLPSLQTVFDLNVRAAVQVTQACLPSLKQSPAGRIVNVCSRAIHGARDRTSYAAAKSALIGVTRTWALELAPLGITANAVAPGPVETELFRLTHPVGSDEEKRILATIPMQRLGKPEEVASLISYLISDGASFVTGQVIGIDGGGSLGGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-10-07" version="64" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LMP9</accession>
    <name>Q1LMP9_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_1696</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08577.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1696"/>
    <dbReference type="EnsemblBacteria" id="ABF08577">
        <property type="protein sequence ID" value="ABF08577"/>
        <property type="gene ID" value="Rmet_1696"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1696"/>
    <dbReference type="eggNOG" id="COG4786">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_078227_0_0_4"/>
    <dbReference type="OMA" id="KKDGLYW"/>
    <dbReference type="OrthoDB" id="1456527at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021556">
        <property type="entry name" value="DUF2950"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11453">
        <property type="entry name" value="DUF2950"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193578" evidence="1">
        <location>
            <begin position="32"/>
            <end position="304"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF08577.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="304" mass="33003" checksum="EF9239A26AA5E624" modified="2006-05-30" version="1">MLALTRILRMAITATAVPLMLMLGLVPAAHAQKSFATPEAAMNAFGDAVTTSNDTAIDTMFGNQARRLIPPLGAEIRYKFLSAWSQSHSIQKQDDNTARIAVGNDGWTMPIPLVRRNGQWQFDTQAGLTEIRLRRIGRNELSTIQTMLAVYDAQREYASTDHVGDGMMVYAAHLVSSPGKRDGLYWPTSAGEPESPLGSAFLRAVGRNASAEGYNGYRYKLLTSQGPAAPGGQYNYLVNGRLFGGFAVLAWPVKYGETGIKSFIVNHEGQVYERDLGKNTSARADGIQSFDPVPGWSKVSATAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-10-07" version="50" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LMT8</accession>
    <name>Q1LMT8_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DDE_Tnp_ISL3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_1655</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08538.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1655"/>
    <dbReference type="EnsemblBacteria" id="ABF08538">
        <property type="protein sequence ID" value="ABF08538"/>
        <property type="gene ID" value="Rmet_1655"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1655"/>
    <dbReference type="eggNOG" id="COG3464">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2938422_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002560">
        <property type="entry name" value="Transposase_DDE"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01610">
        <property type="entry name" value="DDE_Tnp_ISL3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DDE_Tnp_ISL3" evidence="1">
        <location>
            <begin position="4"/>
            <end position="45"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01610"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF08538.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="60" mass="6698" checksum="75F19BE521DBC9D7" modified="2006-05-30" version="1">MALFGQRLQTYWHGTLARCGHPLNTSVVEGINNTIRVIKRRAYGYPVIRSSTTCGWTTSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LNI8</accession>
    <name>Q1LNI8_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Transcriptional regulator, RpiR/GlvR-family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Rmet_1405</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08288.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1405"/>
    <dbReference type="EnsemblBacteria" id="ABF08288">
        <property type="protein sequence ID" value="ABF08288"/>
        <property type="gene ID" value="Rmet_1405"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1405"/>
    <dbReference type="eggNOG" id="COG1737">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_055769_1_0_4"/>
    <dbReference type="OMA" id="DGYPQFR"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097367">
        <property type="term" value="F:carbohydrate derivative binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006096">
        <property type="term" value="P:glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05013">
        <property type="entry name" value="SIS_RpiR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000281">
        <property type="entry name" value="HTH_RpiR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035472">
        <property type="entry name" value="RpiR-like_SIS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001347">
        <property type="entry name" value="SIS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046348">
        <property type="entry name" value="SIS_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01418">
        <property type="entry name" value="HTH_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01380">
        <property type="entry name" value="SIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53697">
        <property type="entry name" value="SSF53697"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51071">
        <property type="entry name" value="HTH_RPIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51464">
        <property type="entry name" value="SIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="1" id="KW-0418">Kinase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="HTH rpiR-type" evidence="3">
        <location>
            <begin position="42"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="domain" description="SIS" evidence="4">
        <location>
            <begin position="165"/>
            <end position="304"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABF08288.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="337" mass="36477" checksum="7F8542F5F325A34B" modified="2006-05-30" version="1">MVAPGKCFFIKARCRSWCANHCFALIRAVLNGSCSPEMPDGLLISERIARALPTLTPAHQRMAQYVLENPFRAATMRIDEFATAVDVSVATANRFARALGFEGYPQFRTELVRGFEATLAPVERLRDELARPTTAAEVFAASLEDGIRNLEATRRALDPEACERAVSAILEAERVYVMGFGASGFLAGLLQHGLEMHCRMVTSVSNSGGASHAGRQLFKLQPQDLVIAIAFPRYVTDTIMLAQRVKEHGGRLMALTDGPASPLAPLADYTLYANTGYRFSANSDATVLALIEALCGAVAHRSARSVKAAAEMTEFLLPWLHGAPDARGGRIAPKSPD</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LNK9</accession>
    <name>Q1LNK9_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Predicted hydrolase or acyltransferase</fullName>
            <ecNumber evidence="2">3.-.-.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_1384</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EC" id="3.-.-.-" evidence="2"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08267.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516145.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1384"/>
    <dbReference type="ESTHER" id="ralme-q1lnk9">
        <property type="family name" value="Epoxide_hydrolase"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF08267">
        <property type="protein sequence ID" value="ABF08267"/>
        <property type="gene ID" value="Rmet_1384"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1384"/>
    <dbReference type="eggNOG" id="COG2267">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020336_7_3_4"/>
    <dbReference type="OMA" id="YRANFIA"/>
    <dbReference type="OrthoDB" id="1439833at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016746">
        <property type="term" value="F:acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00561">
        <property type="entry name" value="Abhydrolase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="SSF53474"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="AB hydrolase-1" evidence="1">
        <location>
            <begin position="39"/>
            <end position="280"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF08267.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="320" mass="35610" checksum="4858DFB09C62130F" modified="2006-05-30" version="1">MTPVDSAHPEFPPPAPTHFAVQSGPVRLAATAWGDPGHPVVVLVHGYPDNSHVWHGVAQALAGRFRVVAYDVRGAGASDVPAGVADYRLERLADDFIAVIDAVSPDRPVHLVGHDWGSIQGWEFVTDPRLYGRIASFTSCSGPCLDHAARALREPPRHWGATLRQALASWYIVFFHIPGLPEWNWKLWLGRNWTFWLRRTEGLHTAANPTQTADGVHGLRLYRANIRTRLRHPHNRHAHAPVQVIVPTRDRYVRPAVTEGVGRWAAQSWRRPVDAHHWFPLADPHRMAAMVREFVDHLQGAPASPALQSARVTSEAGKIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LNP9</accession>
    <name>Q1LNP9_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">ATPase involved in conjugal transfer CagE TrbE VirB component of type IV transporter system (Protein belongs to CMGI-2)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">trbE</name>
        <name evidence="3" type="ordered locus">Rmet_1344</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TrbE/VirB4 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08227.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516108.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1344"/>
    <dbReference type="EnsemblBacteria" id="ABF08227">
        <property type="protein sequence ID" value="ABF08227"/>
        <property type="gene ID" value="Rmet_1344"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1344"/>
    <dbReference type="eggNOG" id="COG3451">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008341_1_0_4"/>
    <dbReference type="OMA" id="PAANYPH"/>
    <dbReference type="OrthoDB" id="182908at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018145">
        <property type="entry name" value="CagE_TrbE_VirB_cntrl_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043964">
        <property type="entry name" value="P-loop_TraG"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03135">
        <property type="entry name" value="CagE_TrbE_VirB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19044">
        <property type="entry name" value="P-loop_TraG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA" evidence="2">
        <location>
            <begin position="440"/>
            <end position="704"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF08227.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="819" mass="90723" checksum="6B6835803E9EAD9E" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LNR9</accession>
    <name>Q1LNR9_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Metapyrocatechase</fullName>
            <ecNumber evidence="4">1.13.11.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">CatO2ase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">Catechol 2,3-dioxygenase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="primary">dmpB</name>
        <name evidence="14" type="ordered locus">Rmet_1324</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>catechol + O2 = (2Z,4E)-2-hydroxy-6-oxohexa-2,4-dienoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17337"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:18135"/>
            <dbReference type="ChEBI" id="CHEBI:71198"/>
            <dbReference type="EC" id="1.13.11.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 12">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3 12">Belongs to the extradiol ring-cleavage dioxygenase family.</text>
    </comment>
    <dbReference type="EC" id="1.13.11.2" evidence="4"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08207.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516090.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1324"/>
    <dbReference type="EnsemblBacteria" id="ABF08207">
        <property type="protein sequence ID" value="ABF08207"/>
        <property type="gene ID" value="Rmet_1324"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1324"/>
    <dbReference type="eggNOG" id="COG0346">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_052361_3_0_4"/>
    <dbReference type="OMA" id="PRRQTLW"/>
    <dbReference type="OrthoDB" id="1335734at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018577">
        <property type="term" value="F:catechol 2,3-dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008198">
        <property type="term" value="F:ferrous iron binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019439">
        <property type="term" value="P:aromatic compound catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.180.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017624">
        <property type="entry name" value="Catechol_2-3_dOase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029068">
        <property type="entry name" value="Glyas_Bleomycin-R_OHBP_Dase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004360">
        <property type="entry name" value="Glyas_Fos-R_dOase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037523">
        <property type="entry name" value="VOC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000486">
        <property type="entry name" value="Xdiol_ring_cleave_dOase_1/2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00903">
        <property type="entry name" value="Glyoxalase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54593">
        <property type="entry name" value="SSF54593"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03211">
        <property type="entry name" value="catechol_2_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00082">
        <property type="entry name" value="EXTRADIOL_DIOXYGENAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51819">
        <property type="entry name" value="VOC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 12" id="KW-0058">Aromatic hydrocarbons catabolism</keyword>
    <keyword evidence="8 12 14" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="12" id="KW-0408">Iron</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9 12 14" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="VOC" evidence="13">
        <location>
            <begin position="5"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="domain" description="VOC" evidence="13">
        <location>
            <begin position="153"/>
            <end position="275"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008784"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022797"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00030369"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00031146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000683"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ABF08207.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="314" mass="35317" checksum="1C0EEE7B64282611" modified="2006-05-30" version="1">MGVMRIGHASLKVMDMALAIKHYENVLGMKRTMEDEHGNVYLKCWDEWDKYSVILTPSDQAGLNHVAYKVEHDADLDALQKRIEAYGFKTQMLPEGTLPSTGRMLQFNLPSGHEMRLFATKEYVGTGVGTTNPDPWPDDVKGAGAHWLDHCLLMCELNPETGVNRVAENTRFMKECLDFYLAEQVMVGPDSSIQAATWMFRTSTPHDIAFVGGPRNGLHHIAFFLDSWHDVLKSADVMAKNKVKIDVAPTRHGITRGETIYFFDPSGNRNETFAGLGYLAQPDRPVTTWTEEHLGSGIFYHTGELVQSFTEVYT</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LP00</accession>
    <name>Q1LP00_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Tyrosine-based site-specific recombinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">ritB2</name>
        <name evidence="4" type="ordered locus">Rmet_1240</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08126.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516013.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1240"/>
    <dbReference type="EnsemblBacteria" id="ABF08126">
        <property type="protein sequence ID" value="ABF08126"/>
        <property type="gene ID" value="Rmet_1240"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1240"/>
    <dbReference type="eggNOG" id="COG4974">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027562_10_0_4"/>
    <dbReference type="OMA" id="GCKFACE"/>
    <dbReference type="OrthoDB" id="745068at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.443.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011010">
        <property type="entry name" value="DNA_brk_join_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013762">
        <property type="entry name" value="Integrase-like_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002104">
        <property type="entry name" value="Integrase_catalytic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010998">
        <property type="entry name" value="Integrase_recombinase_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00589">
        <property type="entry name" value="Phage_integrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56349">
        <property type="entry name" value="SSF56349"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51898">
        <property type="entry name" value="TYR_RECOMBINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tyr recombinase" evidence="3">
        <location>
            <begin position="111"/>
            <end position="315"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF08126.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="330" mass="37268" checksum="F290126EC50D5E29" modified="2006-05-30" version="1">MTAHWNGYHSALATHIEQYLRAKRAMGCKFACEGRQLRLLDRFLAERGIDKIDVIDGACLQAFLDSRHRTNPRSYNNLLGDVRRLFDWIVGQQMLPASPLVAKAKPQRARRIPFLFSPEVIRRLLALARALPDNSRAPQRGITYEMIFALLASLGLRVGEVAGLQWGDVDLEREVLEISNTKFGKDRLVPFGPKLAARLREYRLERERQGRICTGAHHLFSWNGRTPISTNSIRNTFRDDLLPQLHLTVPPGCFGPHVHGLRHSFAVRTLLGWYRQGIIPSDRLHHLSTFLGHVNPKSTAVYLTITNELLDAANERFESFAPLLAAGGSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="111" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LP74</accession>
    <name>Q1LP74_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 8 10">Integration host factor subunit alpha</fullName>
            <shortName evidence="8">IHF-alpha</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8 12" type="primary">ihfA</name>
        <name evidence="8" type="synonym">himA</name>
        <name evidence="12" type="ordered locus">Rmet_1166</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8 10">This protein is one of the two subunits of integration host factor, a specific DNA-binding protein that functions in genetic recombination as well as in transcriptional and translational control.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8 10">Heterodimer of an alpha and a beta chain.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 8 9">Belongs to the bacterial histone-like protein family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08052.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008650476.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1166"/>
    <dbReference type="EnsemblBacteria" id="ABF08052">
        <property type="protein sequence ID" value="ABF08052"/>
        <property type="gene ID" value="Rmet_1166"/>
    </dbReference>
    <dbReference type="GeneID" id="60822036"/>
    <dbReference type="KEGG" id="rme:Rmet_1166"/>
    <dbReference type="eggNOG" id="COG0776">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_105066_1_0_4"/>
    <dbReference type="OMA" id="IYVRGFG"/>
    <dbReference type="OrthoDB" id="1847472at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030527">
        <property type="term" value="F:structural constituent of chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006417">
        <property type="term" value="P:regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd13835">
        <property type="entry name" value="IHF_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.520.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00380">
        <property type="entry name" value="IHF_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000119">
        <property type="entry name" value="Hist_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020816">
        <property type="entry name" value="Histone-like_DNA-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010992">
        <property type="entry name" value="IHF-like_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005684">
        <property type="entry name" value="IHF_alpha"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33175">
        <property type="entry name" value="PTHR33175"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00216">
        <property type="entry name" value="Bac_DNA_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01727">
        <property type="entry name" value="DNABINDINGHU"/>
    </dbReference>
    <dbReference type="SMART" id="SM00411">
        <property type="entry name" value="BHL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47729">
        <property type="entry name" value="SSF47729"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00987">
        <property type="entry name" value="himA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00045">
        <property type="entry name" value="HISTONE_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8 10" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="5 8 10" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 8 10" id="KW-0804">Transcription</keyword>
    <keyword evidence="4 8 10" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="3 8 10" id="KW-0810">Translation regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00018329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00380"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003939"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU004485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ABF08052.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="136" mass="14941" checksum="0DC9F38EBCF062EF" modified="2006-05-30" version="1">MNDRDTISMNAADLGDASDRHAASLDDASPEARAAEVPTLTKAELAEMLFDQVGLNKRESKDMVEAFFDVIREALERGDSVKLSGFGNFQLRDKAQRPGRNPKTGEIIPITARRVVTFHASQKLKAQVEERAGLEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="97" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LPK4</accession>
    <name>Q1LPK4_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Peptide chain release factor 2</fullName>
            <shortName evidence="4">RF-2</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4 6" type="primary">prfB</name>
        <name evidence="6" type="ordered locus">Rmet_1036</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Peptide chain release factor 2 directs the termination of translation in response to the peptide chain termination codons UGA and UAA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="PTM">
        <text evidence="4">Methylated by PrmC. Methylation increases the termination efficiency of RF2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the prokaryotic/mitochondrial release factor family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07922.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1036"/>
    <dbReference type="EnsemblBacteria" id="ABF07922">
        <property type="protein sequence ID" value="ABF07922"/>
        <property type="gene ID" value="Rmet_1036"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1036"/>
    <dbReference type="eggNOG" id="COG1186">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036856_6_0_4"/>
    <dbReference type="OMA" id="YVFHPYQ"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016149">
        <property type="term" value="F:translation release factor activity, codon specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00094">
        <property type="entry name" value="Rel_fac_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005139">
        <property type="entry name" value="PCRF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000352">
        <property type="entry name" value="Pep_chain_release_fac_I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045853">
        <property type="entry name" value="Pep_chain_release_fac_I_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004374">
        <property type="entry name" value="PrfB"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03462">
        <property type="entry name" value="PCRF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00472">
        <property type="entry name" value="RF-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00937">
        <property type="entry name" value="PCRF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75620">
        <property type="entry name" value="SSF75620"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00020">
        <property type="entry name" value="prfB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00745">
        <property type="entry name" value="RF_PROK_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 4" id="KW-0488">Methylation</keyword>
    <keyword evidence="3 4" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RF_PROK_I" evidence="5">
        <location>
            <begin position="237"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="modified residue" description="N5-methylglutamine" evidence="4">
        <location>
            <position position="244"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS00745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABF07922.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="357" mass="39889" checksum="1F9E13443FBDC80B" modified="2006-05-30" version="1">MQSPISVPGRKIYGGIFDYDVKVNRLDEVNGLLEDPDVWNNPKRAQDLGKEKKALEGVVQVLGKLTDDLNGTDELFELAREEGDDETLESIEADVSGFEALVADMEFRRMFSGEMDQANAFIDIQAGAGGTEACDWASMLLRQYLKYCERKGFKAEVLEESEGDVAGIKSATIKVEGEYAFGYLRTETGVHRLVRKSPFDSSGGRHTSFSSVFVYPEVDDSFEVEVNPADLRVDTYRASGAGGQHINKTDSAVRITHMPTGIVVQCQNDRSQHRNRAEAMSMLKSRLYEHEMRKRQAEADKLEAGKTDVGWGHQIRSYVLDQSRIKDLRTNVEMSNTQKVLDGDLDPFIEASLKQGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="95" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LQ45</accession>
    <name>Q1LQ45_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Acyl-CoA dehydrogenase</fullName>
            <ecNumber evidence="8">1.3.8.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Rmet_0845</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the acyl-CoA dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.3.8.-" evidence="8"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07731.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515673.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0845"/>
    <dbReference type="EnsemblBacteria" id="ABF07731">
        <property type="protein sequence ID" value="ABF07731"/>
        <property type="gene ID" value="Rmet_0845"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0845"/>
    <dbReference type="eggNOG" id="COG1960">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018204_9_0_4"/>
    <dbReference type="OMA" id="NKERVAC"/>
    <dbReference type="OrthoDB" id="760677at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016627">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-CH group of donors"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.540.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.110.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006091">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046373">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036250">
        <property type="entry name" value="AcylCo_DH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009075">
        <property type="entry name" value="AcylCo_DH/oxidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013786">
        <property type="entry name" value="AcylCoA_DH/ox_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037069">
        <property type="entry name" value="AcylCoA_DH/ox_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009100">
        <property type="entry name" value="AcylCoA_DH/oxidase_NM_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00441">
        <property type="entry name" value="Acyl-CoA_dh_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02770">
        <property type="entry name" value="Acyl-CoA_dh_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02771">
        <property type="entry name" value="Acyl-CoA_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47203">
        <property type="entry name" value="SSF47203"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56645">
        <property type="entry name" value="SSF56645"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0274">FAD</keyword>
    <keyword evidence="3" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Acyl-CoA_dh_N" evidence="7">
        <location>
            <begin position="6"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA_dh_M" evidence="6">
        <location>
            <begin position="127"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA_dh_1" evidence="5">
        <location>
            <begin position="233"/>
            <end position="394"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF02770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF02771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ABF07731.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="398" mass="44299" checksum="B688BFF0A3C1BDC4" modified="2006-05-30" version="1">MDLLLTPELVEFRDTVRAFLREHLPAELRRGQQLTTAMYPEPEISGPWQARLRERGWLVPLWPREWGGTGWSSIERFIFETECALAGAPLVHPMGVRLVGPVILRYGTEAQKQTYLSRILSGQDYWCQGFSEPGAGSDLASLRTRAVRDGDHYVINGSKLWTTHAHHANRMFALVRTSTEGRRQEGISFILIDMETPGISVRPIPTIGGDHDVNEVFFDNVRVPVANRIGAENEGWQCARYLLEFERGAGIFSPRLRSQLKRIGDVLAREAQCDGDTTVTALRRLRFGEVRADLDAFEMLELKTMCSVAPGESPGPVASVLKLRASRLRQAISEMGTTVVGAESLRWRADDAHDEEPSEDEVLRACLLPDFLNARAYTIFGGAAEVQLGIIAKSCLGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-10-07" version="70" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LQ55</accession>
    <name>Q1LQ55_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Extra-cytoplasmic Solute Receptor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">bug</name>
        <name evidence="4" type="ordered locus">Rmet_0835</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Periplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the UPF0065 (bug) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07721.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0835"/>
    <dbReference type="EnsemblBacteria" id="ABF07721">
        <property type="protein sequence ID" value="ABF07721"/>
        <property type="gene ID" value="Rmet_0835"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0835"/>
    <dbReference type="eggNOG" id="COG3181">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045683_0_0_4"/>
    <dbReference type="OMA" id="YSWQGMA"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042597">
        <property type="term" value="C:periplasmic space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005064">
        <property type="entry name" value="BUG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042100">
        <property type="entry name" value="Bug_dom1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42928">
        <property type="entry name" value="PTHR42928"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03401">
        <property type="entry name" value="TctC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017082">
        <property type="entry name" value="YflP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0675">Receptor</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193695" evidence="3">
        <location>
            <begin position="47"/>
            <end position="351"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF07721.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="351" mass="37061" checksum="9A27E3291840FE6C" modified="2006-05-30" version="1">MFQTLSPIAAQGGTRRVSHLSTLLRRCLGVAIALAAGATVSTTALANTTGTTAWPQKAVTVVVPFPAGGSTDTIARMLAAPLNEKLGQPFVIDNRPGATGAIGATFVKRAPADGYTMMVASIGVYAVNPFLQKNLQYDPAKDFDLLTVAVRAPNVLVANPNFPAKTLQELVAYMKKNPGKVSFATSGAGSSDHLTAALFWQKSGTDGLHVPYKGGAPAISDLLAGQVDVSFQNINAVLQHIRSGKLRALAVTSDKRSAVLPNVPTMAEGGVKDVEVYSWQGVAAPKGLPADVKSRLHGAIVTALKDAKMQQKLNEQGFEVVGNSPEQFAEFETQELKRWKTVIEQGKITAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="117" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LQA7</accession>
    <name>Q1LQA7_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Oxygen-dependent coproporphyrinogen-III oxidase</fullName>
            <shortName evidence="5">CPO</shortName>
            <shortName evidence="5">Coprogen oxidase</shortName>
            <shortName evidence="5">Coproporphyrinogenase</shortName>
            <ecNumber evidence="5">1.3.3.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5 6" type="primary">hemF</name>
        <name evidence="6" type="ordered locus">Rmet_0783</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Involved in the heme biosynthesis. Catalyzes the aerobic oxidative decarboxylation of propionate groups of rings A and B of coproporphyrinogen-III to yield the vinyl groups in protoporphyrinogen-IX.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>coproporphyrinogen III + 2 H(+) + O2 = 2 CO2 + 2 H2O + protoporphyrinogen IX</text>
            <dbReference type="Rhea" id="RHEA:18257"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57307"/>
            <dbReference type="ChEBI" id="CHEBI:57309"/>
            <dbReference type="EC" id="1.3.3.3"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="5">Porphyrin-containing compound metabolism; protoporphyrin-IX biosynthesis; protoporphyrinogen-IX from coproporphyrinogen-III (O2 route): step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1 5">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the aerobic coproporphyrinogen-III oxidase family.</text>
    </comment>
    <dbReference type="EC" id="1.3.3.3" evidence="5"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07669.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515623.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0783"/>
    <dbReference type="EnsemblBacteria" id="ABF07669">
        <property type="protein sequence ID" value="ABF07669"/>
        <property type="gene ID" value="Rmet_0783"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0783"/>
    <dbReference type="eggNOG" id="COG0408">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026169_0_1_4"/>
    <dbReference type="OMA" id="HDKGTLF"/>
    <dbReference type="OrthoDB" id="1086432at2"/>
    <dbReference type="UniPathway" id="UPA00251">
        <property type="reaction ID" value="UER00322"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004109">
        <property type="term" value="F:coproporphyrinogen oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042803">
        <property type="term" value="F:protein homodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006782">
        <property type="term" value="P:protoporphyrinogen IX biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1500.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00333">
        <property type="entry name" value="Coprogen_oxidas"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001260">
        <property type="entry name" value="Coprogen_oxidase_aer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036406">
        <property type="entry name" value="Coprogen_oxidase_aer_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018375">
        <property type="entry name" value="Coprogen_oxidase_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10755">
        <property type="entry name" value="PTHR10755"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01218">
        <property type="entry name" value="Coprogen_oxidas"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000166">
        <property type="entry name" value="Coproporphyri_ox"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00073">
        <property type="entry name" value="COPRGNOXDASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102886">
        <property type="entry name" value="SSF102886"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01021">
        <property type="entry name" value="COPROGEN_OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 5" id="KW-0350">Heme biosynthesis</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2 5 6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4 5" id="KW-0627">Porphyrin biosynthesis</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Substrate binding" evidence="5">
        <location>
            <begin position="111"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="region of interest" description="Important for dimerization" evidence="5">
        <location>
            <begin position="243"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="5">
        <location>
            <begin position="261"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="5">
        <location>
            <position position="109"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="95"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="99"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="109"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="148"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="178"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="site" description="Important for dimerization" evidence="5">
        <location>
            <position position="178"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00333"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABF07669.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="303" mass="34225" checksum="CDBD7AC75F3F3F32" modified="2006-05-30" version="1">MIDSQAVRDYLLGLQDRITDAIAAVDGQAFLTDNWEKPPTERLRGSGRTRILEGGAVMERAGVGFSHVRGDTLPPSASANRPELAGRSFEAMGVSLVFHPRNPYVPTVHMNVRCFIAVKPDAEPVWWFGGGMDLTPYYGNADDCAHFHRTCKDSLAPFGDDLYPRFKQWCDEYFFLRHRGEARGIGGIFFDDFSALGFDRSFEMLRSVGDAFLAAYLPILTARKDAAYGERERDFQAYRRGRYVEFNLVFDRGTLFGLQSGGRTESILMSMPPQVSWRYDWQPEPGTPEAALYTDFLPARDWA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-10-17" modified="2022-05-25" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LQD9</accession>
    <name>RL19_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">50S ribosomal protein L19</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rplS</name>
        <name type="ordered locus">Rmet_0751</name>
    </gene>
    <organism>
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">This protein is located at the 30S-50S ribosomal subunit interface and may play a role in the structure and function of the aminoacyl-tRNA binding site.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the bacterial ribosomal protein bL19 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07637.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008643705.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q1LQD9"/>
    <dbReference type="SMR" id="Q1LQD9"/>
    <dbReference type="STRING" id="266264.Rmet_0751"/>
    <dbReference type="PRIDE" id="Q1LQD9"/>
    <dbReference type="EnsemblBacteria" id="ABF07637">
        <property type="protein sequence ID" value="ABF07637"/>
        <property type="gene ID" value="Rmet_0751"/>
    </dbReference>
    <dbReference type="GeneID" id="60825237"/>
    <dbReference type="KEGG" id="rme:Rmet_0751"/>
    <dbReference type="eggNOG" id="COG0335">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_103507_1_0_4"/>
    <dbReference type="OMA" id="TITVYYE"/>
    <dbReference type="OrthoDB" id="1698718at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.790">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00402">
        <property type="entry name" value="Ribosomal_L19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038657">
        <property type="entry name" value="L19_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001857">
        <property type="entry name" value="Ribosomal_L19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018257">
        <property type="entry name" value="Ribosomal_L19_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008991">
        <property type="entry name" value="Translation_prot_SH3-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15680">
        <property type="entry name" value="PTHR15680"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01245">
        <property type="entry name" value="Ribosomal_L19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002191">
        <property type="entry name" value="Ribosomal_L19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00061">
        <property type="entry name" value="RIBOSOMALL19"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50104">
        <property type="entry name" value="SSF50104"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01024">
        <property type="entry name" value="rplS_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01015">
        <property type="entry name" value="RIBOSOMAL_L19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <feature type="chain" id="PRO_0000252530" description="50S ribosomal protein L19">
        <location>
            <begin position="1"/>
            <end position="130"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00402"/>
        </source>
    </evidence>
    <sequence length="130" mass="14511" checksum="2C6582D4F4D68E91" modified="2006-05-30" version="1">MNIIEQIEKEEIARLTANKTIPAFAPGDTVIVNVNVVEGNRKRVQAYEGVVIAKRNRGLNSSFIVRKISSGEGVERTFQLYSPLIAGIEVKRRGDVRRAKLYYLRERSGKSARIKEKLVMKAKPAAVAAE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-01-15" modified="2022-08-03" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LQG9</accession>
    <name>KCY_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Cytidylate kinase</fullName>
            <shortName evidence="1">CK</shortName>
            <ecNumber evidence="1">2.7.4.25</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Cytidine monophosphate kinase</fullName>
            <shortName evidence="1">CMP kinase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">cmk</name>
        <name type="ordered locus">Rmet_0721</name>
    </gene>
    <organism>
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + CMP = ADP + CDP</text>
            <dbReference type="Rhea" id="RHEA:11600"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58069"/>
            <dbReference type="ChEBI" id="CHEBI:60377"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.25"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + dCMP = ADP + dCDP</text>
            <dbReference type="Rhea" id="RHEA:25094"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57566"/>
            <dbReference type="ChEBI" id="CHEBI:58593"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.25"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the cytidylate kinase family. Type 1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.4.25" evidence="1"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07607.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515570.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q1LQG9"/>
    <dbReference type="SMR" id="Q1LQG9"/>
    <dbReference type="STRING" id="266264.Rmet_0721"/>
    <dbReference type="PRIDE" id="Q1LQG9"/>
    <dbReference type="EnsemblBacteria" id="ABF07607">
        <property type="protein sequence ID" value="ABF07607"/>
        <property type="gene ID" value="Rmet_0721"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0721"/>
    <dbReference type="eggNOG" id="COG0283">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_079959_2_0_4"/>
    <dbReference type="OMA" id="RAITWWM"/>
    <dbReference type="OrthoDB" id="776861at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036430">
        <property type="term" value="F:CMP kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036431">
        <property type="term" value="F:dCMP kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006220">
        <property type="term" value="P:pyrimidine nucleotide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02020">
        <property type="entry name" value="CMPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00238">
        <property type="entry name" value="Cytidyl_kinase_type1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003136">
        <property type="entry name" value="Cytidylate_kin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011994">
        <property type="entry name" value="Cytidylate_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02224">
        <property type="entry name" value="Cytidylate_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00017">
        <property type="entry name" value="cmk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0418">Kinase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_1000048258" description="Cytidylate kinase">
        <location>
            <begin position="1"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="11"/>
            <end position="19"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00238"/>
        </source>
    </evidence>
    <sequence length="219" mass="23742" checksum="741669245987B760" modified="2006-05-30" version="1">MTIVDVITIDGPTASGKGTVAHKVADALGFHLLDSGALYRLVALASDRAGVGVQDVDTLAKMAARLDVKFGPDRVWLSGEEVSQTIRLEAIGNRASAIAVHQPVRDALTALQRGFRKLPGLVADGRDMGTVIFPDAPLKVFLTASVEARARRRYKQLIDKGISANIEDLLRDLEERDARDRTRAAAPLRPAEGAYRLDTSEMTVEEAVAQVLEWFAAVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LR10</accession>
    <name>Q1LR10_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">HTH cro/C1-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_0530</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07416.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515392.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0530"/>
    <dbReference type="DNASU" id="4037319"/>
    <dbReference type="EnsemblBacteria" id="ABF07416">
        <property type="protein sequence ID" value="ABF07416"/>
        <property type="gene ID" value="Rmet_0530"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0530"/>
    <dbReference type="eggNOG" id="COG1396">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_057862_2_1_4"/>
    <dbReference type="OMA" id="GVSTTWY"/>
    <dbReference type="OrthoDB" id="922937at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001387">
        <property type="entry name" value="Cro/C1-type_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010982">
        <property type="entry name" value="Lambda_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041413">
        <property type="entry name" value="MLTR_LBD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17765">
        <property type="entry name" value="MLTR_LBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00530">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47413">
        <property type="entry name" value="SSF47413"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH cro/C1-type" evidence="1">
        <location>
            <begin position="17"/>
            <end position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF07416.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="264" mass="29297" checksum="01D2C96442A2D8AF" modified="2006-05-30" version="1">MSIEPTQVSREAELGAFLRTHRERLTPSDVGLPPGRRRRTPGLRREEVAQLSGLSATWITWLEQGRPVAMSVRALGSLAEALRLSRAERAYLFALAGKPEPRQDAEPAVPVALLASVQAISGPAYLLDRQWTARAWNAAAADLFVGWLDAASGERNLLRAMFLPTALQALVEDWPHRAARLVAEFRVHSLRHAEDPPTRALIERLMEDSPAFAAAWRSQDVDERQGGRRGFHHPMRGLVYFEQLTLVPPAAPGMMLVMLLPETP</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="66" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LR50</accession>
    <name>Q1LR50_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">SnoaL-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_0490</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07376.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515361.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0490"/>
    <dbReference type="EnsemblBacteria" id="ABF07376">
        <property type="protein sequence ID" value="ABF07376"/>
        <property type="gene ID" value="Rmet_0490"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0490"/>
    <dbReference type="eggNOG" id="COG4319">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_084893_2_0_4"/>
    <dbReference type="OMA" id="HIHASPM"/>
    <dbReference type="OrthoDB" id="1644255at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032710">
        <property type="entry name" value="NTF2-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037401">
        <property type="entry name" value="SnoaL-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13474">
        <property type="entry name" value="SnoaL_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54427">
        <property type="entry name" value="SSF54427"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SnoaL-like" evidence="1">
        <location>
            <begin position="13"/>
            <end position="129"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13474"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF07376.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="146" mass="16164" checksum="5B7AD267CBFF45D5" modified="2006-05-30" version="1">MPRFARLFDSAEDIVDAFREAMKLRDADGALRLWLDEDSVTCVMPDGQRLIGHDQLRQAFALLLEEQPVLIDAIETSSHASLAVSIFDVTEALRFGTDRVEADLYVHTTYVLMQNHEGWRLAHIHCSPANAVQVAAVAAAPGQALH</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-01-15" modified="2022-05-25" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LRC9</accession>
    <name>RS9_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">30S ribosomal protein S9</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpsI</name>
        <name type="ordered locus">Rmet_0411</name>
    </gene>
    <organism>
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uS9 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07297.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008646004.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q1LRC9"/>
    <dbReference type="SMR" id="Q1LRC9"/>
    <dbReference type="STRING" id="266264.Rmet_0411"/>
    <dbReference type="EnsemblBacteria" id="ABF07297">
        <property type="protein sequence ID" value="ABF07297"/>
        <property type="gene ID" value="Rmet_0411"/>
    </dbReference>
    <dbReference type="GeneID" id="60824013"/>
    <dbReference type="KEGG" id="rme:Rmet_0411"/>
    <dbReference type="eggNOG" id="COG0103">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046483_2_1_4"/>
    <dbReference type="OMA" id="QWKINGR"/>
    <dbReference type="OrthoDB" id="1766414at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00532_B">
        <property type="entry name" value="Ribosomal_S9_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000754">
        <property type="entry name" value="Ribosomal_S9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023035">
        <property type="entry name" value="Ribosomal_S9_bac/plastid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020574">
        <property type="entry name" value="Ribosomal_S9_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21569">
        <property type="entry name" value="PTHR21569"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00380">
        <property type="entry name" value="Ribosomal_S9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="SSF54211"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00360">
        <property type="entry name" value="RIBOSOMAL_S9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <feature type="chain" id="PRO_1000051302" description="30S ribosomal protein S9">
        <location>
            <begin position="1"/>
            <end position="130"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00532"/>
        </source>
    </evidence>
    <sequence length="130" mass="14484" checksum="6964B97FE30D508D" modified="2006-05-30" version="1">MIGNWNYGTGRRKSAVARVFIKSGKGDIIVNGKPIKEYFARETSLMIVRQPLELTAHAETFDIKVNVTGGGETGQAGAVRHGITRALIDYDATLKPTLSKAGYVTRDAREVERKKVGLHKARRRKQFSKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="101" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LRN7</accession>
    <name>Q1LRN7_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">RNA polymerase sigma-54 factor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">rpoN</name>
        <name evidence="13" type="ordered locus">Rmet_0303</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 9">Belongs to the sigma-54 factor family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07189.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515189.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0303"/>
    <dbReference type="EnsemblBacteria" id="ABF07189">
        <property type="protein sequence ID" value="ABF07189"/>
        <property type="gene ID" value="Rmet_0303"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0303"/>
    <dbReference type="eggNOG" id="COG1508">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020569_0_1_4"/>
    <dbReference type="OMA" id="VTTQKFM"/>
    <dbReference type="OrthoDB" id="1906842at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000428">
        <property type="term" value="C:DNA-directed RNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001216">
        <property type="term" value="F:DNA-binding transcription activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016779">
        <property type="term" value="F:nucleotidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016987">
        <property type="term" value="F:sigma factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006352">
        <property type="term" value="P:DNA-templated transcription, initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.1330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000394">
        <property type="entry name" value="RNA_pol_sigma_54"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007046">
        <property type="entry name" value="RNA_pol_sigma_54_core-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007634">
        <property type="entry name" value="RNA_pol_sigma_54_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038709">
        <property type="entry name" value="RpoN_core-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32248">
        <property type="entry name" value="PTHR32248"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00309">
        <property type="entry name" value="Sigma54_AID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04963">
        <property type="entry name" value="Sigma54_CBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04552">
        <property type="entry name" value="Sigma54_DBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000774">
        <property type="entry name" value="RpoN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00045">
        <property type="entry name" value="SIGMA54FCT"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02395">
        <property type="entry name" value="rpoN_sigma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00717">
        <property type="entry name" value="SIGMA54_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00718">
        <property type="entry name" value="SIGMA54_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2 9" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="4 9" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9" id="KW-0731">Sigma factor</keyword>
    <keyword evidence="8 9" id="KW-0804">Transcription</keyword>
    <keyword evidence="5 9" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="3 9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Sigma54_CBD" evidence="12">
        <location>
            <begin position="129"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="domain" description="Sigma54_DBD" evidence="11">
        <location>
            <begin position="335"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="66"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="69"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="109"/>
            <end position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008798"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR000774"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF04552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF04963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ABF07189.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="492" mass="54773" checksum="3AD0CCC1F41DA088" modified="2006-05-30" version="1">MKPSLQLRLSQHLALTPQLQQSIRLLQLSTLELQQEVEQALTENPLLERENDWIESPLRVAADGSVNVQSAPAPASSEPQGNGDDRANGANGEDDSYGDSAGDDYGSDWSLDDFARRPQGDDDEKTPMQLREAEPTLREYLMEQLTPLKISTRDKGLAIFLIESLDDDGYLGATLDEICSELPKELEFEVDEVQAILTLLQSFDPPGVGARNAAECLTLQLRRLQHPQRDLALSIVNHHLELLAVRDYTRLKKALQVDEIALKAAHDLIRSLAPYPGHAYSRPEADFVVPDVFVRKSGSSWIAQLNPDVMPRLRINDMYAQILRGAKGEAGAAGLQQKLQEARWLIKNIQQRFDTILRVAQAIVERQRNFFSHGEIAMRPLVLREIADTLGLHESTISRVTTNKYMATPMGTFELKYFFGSHVSTETGGAASSTAIRALIKQLIGAEEPRNPLSDSRIAELLGEQGFVVARRTVAKYREALKIPAVNLRKTL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-01-15" modified="2022-08-03" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LS47</accession>
    <name>METXS_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Homoserine O-succinyltransferase</fullName>
            <shortName evidence="1">HST</shortName>
            <ecNumber evidence="1">2.3.1.46</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Homoserine transsuccinylase</fullName>
            <shortName evidence="1">HTS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">metXS</name>
        <name type="ordered locus">Rmet_0143</name>
    </gene>
    <organism>
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Transfers a succinyl group from succinyl-CoA to L-homoserine, forming succinyl-L-homoserine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>L-homoserine + succinyl-CoA = CoA + O-succinyl-L-homoserine</text>
            <dbReference type="Rhea" id="RHEA:22008"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57292"/>
            <dbReference type="ChEBI" id="CHEBI:57476"/>
            <dbReference type="ChEBI" id="CHEBI:57661"/>
            <dbReference type="EC" id="2.3.1.46"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; O-succinyl-L-homoserine from L-homoserine: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the AB hydrolase superfamily. MetX family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.46" evidence="1"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07029.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008647216.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q1LS47"/>
    <dbReference type="SMR" id="Q1LS47"/>
    <dbReference type="STRING" id="266264.Rmet_0143"/>
    <dbReference type="ESTHER" id="ralme-q1ls47">
        <property type="family name" value="Homoserine_transacetylase"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF07029">
        <property type="protein sequence ID" value="ABF07029"/>
        <property type="gene ID" value="Rmet_0143"/>
    </dbReference>
    <dbReference type="GeneID" id="60822866"/>
    <dbReference type="KEGG" id="rme:Rmet_0143"/>
    <dbReference type="eggNOG" id="COG2021">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028760_1_2_4"/>
    <dbReference type="OMA" id="TRFCVVS"/>
    <dbReference type="OrthoDB" id="536745at2"/>
    <dbReference type="UniPathway" id="UPA00051">
        <property type="reaction ID" value="UER00075"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008899">
        <property type="term" value="F:homoserine O-succinyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009086">
        <property type="term" value="P:methionine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00296">
        <property type="entry name" value="MetX_acyltransf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008220">
        <property type="entry name" value="HAT_MetX-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32268">
        <property type="entry name" value="PTHR32268"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00561">
        <property type="entry name" value="Abhydrolase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000443">
        <property type="entry name" value="Homoser_Ac_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="SSF53474"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01392">
        <property type="entry name" value="homoserO_Ac_trn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0012">Acyltransferase</keyword>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0486">Methionine biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_1000021900" description="Homoserine O-succinyltransferase">
        <location>
            <begin position="1"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="domain" description="AB hydrolase-1" evidence="1">
        <location>
            <begin position="59"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="1">
        <location>
            <position position="165"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="330"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="363"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="235"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="364"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Important for acyl-CoA specificity" evidence="1">
        <location>
            <position position="332"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00296"/>
        </source>
    </evidence>
    <sequence length="390" mass="43010" checksum="3F656780DF96A696" modified="2006-05-30" version="1">MTDVAPPAGVLDVPTDSVGVVTPQRMHFSEPLQLRNGSQIADYDLMVETYGTLNAARTNAVLVCHALNASHHVAGIAADNPRDIGWWDNMVGPGKPLDTNRFFVIGVNNLGSCFGSTGPMSTNPSTGAPYGALFPVVTVEDWVNAQARVADIFGIQQFAAVMGGSLGGMQALAWSLMYPERLRHCIVVASTPKLSAQNIAFNEVARSSILSDPDFHGGNYYAHNVKPKRGLRVARMIGHITYLSDEDMAEKFGRSLKAEDIRFSFDVEFQVESYLRYQGDKFAEYFDANTYLLITRALDYFDPALAHGGDLTRAMAQTQAGFLVVSFTTDWRFAPNRSREIVKALLDNKRPVSYAEIDAPHGHDAFLLDDARYHNLMRAYYDRIAEEIGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="98" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LS48</accession>
    <name>Q1LS48_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Transcriptional regulator, TetR family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">ttk</name>
        <name evidence="4" type="ordered locus">Rmet_0142</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07028.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515058.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0142"/>
    <dbReference type="EnsemblBacteria" id="ABF07028">
        <property type="protein sequence ID" value="ABF07028"/>
        <property type="gene ID" value="Rmet_0142"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0142"/>
    <dbReference type="eggNOG" id="COG1309">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069356_5_0_4"/>
    <dbReference type="OMA" id="IEQTVFG"/>
    <dbReference type="OrthoDB" id="1437290at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001647">
        <property type="entry name" value="HTH_TetR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036271">
        <property type="entry name" value="Tet_transcr_reg_TetR-rel_C_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00440">
        <property type="entry name" value="TetR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48498">
        <property type="entry name" value="SSF48498"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50977">
        <property type="entry name" value="HTH_TETR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH tetR-type" evidence="3">
        <location>
            <begin position="31"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="H-T-H motif" evidence="1">
        <location>
            <begin position="54"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF07028.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="216" mass="23800" checksum="6A132E1ACD61ABDE" modified="2006-05-30" version="1">MTQTISSQAEADTPAGHSPEAAPARKRPRPGERRIQILQTLAGMLEHPRGEKITTAALAAKLSVSEAALYRHFASKAQMFEGLIGFIEQTVFGLINQITTQEEHGLRQAQAIVRMLLSFAEKNPGMTRVLTGEALVGEHERLQERINQMIDRIEASLRQCLKVAVSQAAFPPDADIPLRVALITAAVQGHWHRFVKSGFRKAPTENIDAQLRILLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LS78</accession>
    <name>Q1LS78_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Conserved hypothetical signal peptide protein</fullName>
            <ecNumber evidence="4">3.1.1.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Rmet_0112</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EC" id="3.1.1.-" evidence="4"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF06998.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515032.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0112"/>
    <dbReference type="ESTHER" id="ralme-q1ls78">
        <property type="family name" value="Prolyl_oligopeptidase_S9"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF06998">
        <property type="protein sequence ID" value="ABF06998"/>
        <property type="gene ID" value="Rmet_0112"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0112"/>
    <dbReference type="eggNOG" id="COG0412">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_043246_0_0_4"/>
    <dbReference type="OMA" id="DNDSYWP"/>
    <dbReference type="OrthoDB" id="721431at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008236">
        <property type="term" value="F:serine-type peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001375">
        <property type="entry name" value="Peptidase_S9"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00326">
        <property type="entry name" value="Peptidase_S9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="SSF53474"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193798" evidence="2">
        <location>
            <begin position="28"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase_S9" evidence="3">
        <location>
            <begin position="92"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="290"/>
            <end position="309"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF06998.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="309" mass="33399" checksum="B2E82247E366DAF6" modified="2006-05-30" version="1">MKSRVVSSICGIAAALAGLVWCATAIAQVALPAHTIEQVVMVPKVGAEDTVDLETTIFRPPGEGRRPLVVINHGKTPGKPAFQRRARFAAQTAEFVRRGFVVALPMRQGFSKSGGKYVGGSCDVGANGMAQAEDVIAALDYLVQQPYVDPTRIVVIGQSFGGLSTMALSTIGYPGVVGVINFAGGLRNESCSGWENNLVDVFGDYGRGARYPSLWIYGDNDRFWPWPLPERMFNSYKANVSGAAKDTHFVDVGEFDGDSHQLFSDSAGIAVWLPEVETFFRDHGLPFDVEPPEAEPVTRGVTSNRRQKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="96" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LS84</accession>
    <name>Q1LS84_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Acetyl-CoA acetyltransferase</fullName>
            <ecNumber evidence="6">2.3.1.9</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">atoB</name>
        <name evidence="6" type="ordered locus">Rmet_0106</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the thiolase-like superfamily. Thiolase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.9" evidence="6"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF06992.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515026.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0106"/>
    <dbReference type="EnsemblBacteria" id="ABF06992">
        <property type="protein sequence ID" value="ABF06992"/>
        <property type="gene ID" value="Rmet_0106"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0106"/>
    <dbReference type="eggNOG" id="COG0183">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031026_0_1_4"/>
    <dbReference type="OMA" id="YEAGRSM"/>
    <dbReference type="OrthoDB" id="1058688at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003985">
        <property type="term" value="F:acetyl-CoA C-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd00751">
        <property type="entry name" value="thiolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.47.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002155">
        <property type="entry name" value="Thiolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016039">
        <property type="entry name" value="Thiolase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020610">
        <property type="entry name" value="Thiolase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020617">
        <property type="entry name" value="Thiolase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020616">
        <property type="entry name" value="Thiolase_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02803">
        <property type="entry name" value="Thiolase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00108">
        <property type="entry name" value="Thiolase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000429">
        <property type="entry name" value="Ac-CoA_Ac_transf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53901">
        <property type="entry name" value="SSF53901"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01930">
        <property type="entry name" value="AcCoA-C-Actrans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00099">
        <property type="entry name" value="THIOLASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Thiolase_N" evidence="4">
        <location>
            <begin position="6"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="domain" description="Thiolase_C" evidence="5">
        <location>
            <begin position="272"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="active site" description="Acyl-thioester intermediate" evidence="2">
        <location>
            <position position="90"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="2">
        <location>
            <position position="350"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="2">
        <location>
            <position position="380"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR000429-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF02803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABF06992.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="394" mass="41356" checksum="122B1D2608546355" modified="2006-05-30" version="1">MQKDPVVIVSAVRTPMGAFQSELSSLMAPQLGAAAIRAAVERAGIKPEQVQEVVFGCVLPAGQGQAPARQAALGAGLPLDVACTTVNKMCGSGMRAAMSVYDGLLAGSFDIAVAGGMESMTNAPYLVPKARGGYRIGHGMIYDHMMLDGLEDAYDKGRAMGTFGEDCAAKYHFTREQQDAFAMESVRRAQRATEQGDFRWEIVPVTVSGKGGDTVIDTDEGPRRIKVDKIPSLKPAFAKDGTITAASSSSINDGASALVMMRESTAKSLGLQPIARMLGHTSHAQAPGWFTTAPVEAIAKLYRKLDWNTGSVDLFEINEAFAVVPMAAMHDLDIPRDKVNIHGGACALGHPIGASGARIMTTLIGALRKTGGKRGVASLCIGGGEATAVGIELY</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="103" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LSI3</accession>
    <name>Q1LSI3_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transcriptional regulator, araC family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_0007</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF06893.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011514941.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0007"/>
    <dbReference type="EnsemblBacteria" id="ABF06893">
        <property type="protein sequence ID" value="ABF06893"/>
        <property type="gene ID" value="Rmet_0007"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0007"/>
    <dbReference type="eggNOG" id="COG2207">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_88_16_4"/>
    <dbReference type="OMA" id="RAWRMLY"/>
    <dbReference type="OrthoDB" id="1268974at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003313">
        <property type="entry name" value="AraC-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037923">
        <property type="entry name" value="HTH-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018060">
        <property type="entry name" value="HTH_AraC"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02311">
        <property type="entry name" value="AraC_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12833">
        <property type="entry name" value="HTH_18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00342">
        <property type="entry name" value="HTH_ARAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51215">
        <property type="entry name" value="SSF51215"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01124">
        <property type="entry name" value="HTH_ARAC_FAMILY_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH araC/xylS-type" evidence="1">
        <location>
            <begin position="171"/>
            <end position="268"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS01124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF06893.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="272" mass="29565" checksum="C82A08381655FEBA" modified="2006-05-30" version="1">MAGGQFQIFRCHVAGTQAVQADSRHAFGRHTHDQFGIGVIGKGAQRSASGQGIVEAGPGDTITVNPGEVHDGMPIGDAGRAWQMLYFDPPVVADAILDVTEGRTRSFEFAHPVMRDVRLAQRFLTLFTAMTAAHAEHASVLREELLLTLLADTMHGQTRSDTRGPGFAEIALARRRIDDDSAAPLTLGELANECRLSRFQFIRAFSRATGMTPHAYMIQRRIIAARHLIARRMSLADAAAASGFADQSHMTRIFVRTYGISPSMYARACGAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-04-26" modified="2022-05-25" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>Q58AE2</accession>
    <name>Q58AE2_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">CopG involved in survival in presence of high bioavailable Cu(II)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">copG</name>
        <name evidence="2" type="ordered locus">Rmet_6118</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2 3">
        <name>pMOL30</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid pMOL30</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000354">
        <property type="protein sequence ID" value="ABF12977.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011229399.1">
        <property type="nucleotide sequence ID" value="NC_007971.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_145676.1">
        <property type="nucleotide sequence ID" value="NC_006466.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF12977">
        <property type="protein sequence ID" value="ABF12977"/>
        <property type="gene ID" value="Rmet_6118"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6118"/>
    <dbReference type="HOGENOM" id="CLU_112034_0_0_4"/>
    <dbReference type="OMA" id="CCKKWVE"/>
    <dbReference type="OrthoDB" id="1676023at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid pMOL30"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007332">
        <property type="entry name" value="DUF411"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04214">
        <property type="entry name" value="DUF411"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="SSF52833"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5013531912" evidence="1">
        <location>
            <begin position="19"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF12977.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="137" mass="14134" checksum="094286A6660C4BD3" modified="2005-04-26" version="1">MKYLFAAAILAGSAASYAAAPAMTVYKDPNCGCCEEWVKHINQAGFPPKVINSTDVTSVKTRLGVPASFSSCHTAVLETSGQVIEGHVPASAIQKLVANRAVKGVAVPGMPTNAPGMGKLNGGLVTVDFQGKPFSQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-04-26" modified="2021-09-29" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>Q58AH3</accession>
    <name>Q58AH3_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_6163</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2 3">
        <name>pMOL30</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid pMOL30</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000354">
        <property type="protein sequence ID" value="ABF13022.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011229377.1">
        <property type="nucleotide sequence ID" value="NC_007971.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_145645.1">
        <property type="nucleotide sequence ID" value="NC_006466.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF13022">
        <property type="protein sequence ID" value="ABF13022"/>
        <property type="gene ID" value="Rmet_6163"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6163"/>
    <dbReference type="HOGENOM" id="CLU_066864_0_0_4"/>
    <dbReference type="OMA" id="FSWVSKY"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid pMOL30"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021496">
        <property type="entry name" value="DUF3150"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11348">
        <property type="entry name" value="DUF3150"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="296"/>
            <end position="355"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF13022.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="355" mass="38259" checksum="CF3499BDBB9DAC56" modified="2005-04-26" version="1">MNQATTVASSQMAILKDVVLFDFIIGGSTGEKTVEADDFIREVGKMLPRGSFSWVSKYRSEAKREILKVGVARRIHNRVRGYFVPLSEAAGLSSKMTDIKARFMKEKAEFLANLPRIVDEWANAPVNASSTNNGKSRADLIRLHAPKTDDMDRMLSFDISATGIQSTSFFGEDDALHAEVKGLVGQAALEIAEDVRKSWKGPAAGRTSSRVLGLIRRVRNKAEAMGVLSMKFSRLAEVCDKVLGAVPEGQNIEGVQFLMVSSLLSRCMKAENILSEDSVKFDPLDVESAAPAVTPGVQPPAANVPMQSQPDLAIGDTAPAFDESPQPSSPLPTAILPSAAEPVASTQDDTLELVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-04-26" modified="2022-08-03" version="117" xmlns="http://uniprot.org/uniprot">
    <accession>Q58AI1</accession>
    <accession>Q1L9H1</accession>
    <name>Q58AI1_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Periplasmic mercury ion-binding protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7 11" type="primary">merP</name>
        <name evidence="10" type="ordered locus">Rmet_6173</name>
        <name evidence="11" type="ordered locus">Rmet_6346</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="11 12">
        <name>pMOL28</name>
    </geneLocation>
    <geneLocation type="plasmid" evidence="10 12">
        <name>pMOL30</name>
    </geneLocation>
    <reference evidence="11" key="1">
        <citation type="submission" date="2006-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of the chromosome of Ralstonia metallidurans CH34.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Copeland A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Barry K."/>
                <person name="Detter J.C."/>
                <person name="Glavina del Rio T."/>
                <person name="Hammon N."/>
                <person name="Israni S."/>
                <person name="Dalin E."/>
                <person name="Tice H."/>
                <person name="Martinez M."/>
                <person name="Goltsman E."/>
                <person name="Pitluck S."/>
                <person name="Schmutz J."/>
                <person name="Larimer F."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Kim E."/>
                <person name="Mergeay M."/>
                <person name="Benotmane M.A."/>
                <person name="Vallaeys T."/>
                <person name="Michaux A."/>
                <person name="Monchy S."/>
                <person name="Dunn J."/>
                <person name="McCorkle S."/>
                <person name="Taghavi S."/>
                <person name="van der Lelie D."/>
                <person name="Richardson P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">CH34</strain>
            <plasmid evidence="11">pMOL28</plasmid>
            <plasmid evidence="10">pMOL30</plasmid>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="12">Plasmid pMOL28</plasmid>
            <plasmid evidence="12">Plasmid pMOL30</plasmid>
        </source>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2010-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Benotmane R."/>
                <person name="Lapidus A."/>
                <person name="McCorkle S."/>
                <person name="Monchy S."/>
                <person name="Taghavi S."/>
                <person name="van der Lelie N."/>
                <person name="Dunn J."/>
                <person name="Leys N."/>
                <person name="Mergeay M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">CH34</strain>
            <plasmid evidence="11">pMOL28</plasmid>
            <plasmid evidence="10">pMOL30</plasmid>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Involved in mercury resistance. Acts as a mercury scavenger that specifically binds to a mercuric ion in the periplasm and probably passes it to the cytoplasmic mercuric reductase MerA via the mercuric transport protein MerT.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Periplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MerP family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000354">
        <property type="protein sequence ID" value="ABF13032.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP000355">
        <property type="protein sequence ID" value="ABF13205.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_003131987.1">
        <property type="nucleotide sequence ID" value="NC_007972.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_145637.1">
        <property type="nucleotide sequence ID" value="NC_006466.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF13032">
        <property type="protein sequence ID" value="ABF13032"/>
        <property type="gene ID" value="Rmet_6173"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF13205">
        <property type="protein sequence ID" value="ABF13205"/>
        <property type="gene ID" value="Rmet_6346"/>
    </dbReference>
    <dbReference type="GeneID" id="61089174"/>
    <dbReference type="KEGG" id="rme:Rmet_6173"/>
    <dbReference type="KEGG" id="rme:Rmet_6346"/>
    <dbReference type="HOGENOM" id="CLU_134973_2_1_4"/>
    <dbReference type="OMA" id="CNHCKAS"/>
    <dbReference type="OrthoDB" id="2061355at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid pMOL28"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid pMOL30"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042597">
        <property type="term" value="C:periplasmic space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045340">
        <property type="term" value="F:mercury ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015097">
        <property type="term" value="F:mercury ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00371">
        <property type="entry name" value="HMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017969">
        <property type="entry name" value="Heavy-metal-associated_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006121">
        <property type="entry name" value="HMA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036163">
        <property type="entry name" value="HMA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011795">
        <property type="entry name" value="MerP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001802">
        <property type="entry name" value="MerP/CopZ"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00403">
        <property type="entry name" value="HMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00946">
        <property type="entry name" value="HGSCAVENGER"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55008">
        <property type="entry name" value="SSF55008"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02052">
        <property type="entry name" value="MerP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01047">
        <property type="entry name" value="HMA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50846">
        <property type="entry name" value="HMA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0475">Mercuric resistance</keyword>
    <keyword evidence="7" id="KW-0476">Mercury</keyword>
    <keyword evidence="4 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 7" id="KW-0574">Periplasm</keyword>
    <keyword evidence="11" id="KW-0614">Plasmid</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5013531909" description="Periplasmic mercury ion-binding protein" evidence="8">
        <location>
            <begin position="20"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="domain" description="HMA" evidence="9">
        <location>
            <begin position="23"/>
            <end position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005938"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022764"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU361212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ABF13032.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ABF13205.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="91" mass="9490" checksum="C7CDB86B5FC74D78" modified="2005-04-26" version="1">MKKLFASLALAAVVAPVWAATQTVTLSVPGMTCSACPITVKKAISKVEGVSKVDVTFETRQAVVTFDDAKTSVQKLTKATADAGYPSSVKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-01-04" modified="2022-08-03" version="119" xmlns="http://uniprot.org/uniprot">
    <accession>Q5NUV1</accession>
    <name>Q5NUV1_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">HTH-type transcriptional regulator MerD</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">Mercuric resistance protein MerD</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="4" type="primary">merD</name>
        <name evidence="4" type="ordered locus">Rmet_6175</name>
        <name evidence="5" type="ordered locus">Rmet_6184</name>
    </gene>
    <organism evidence="4 6">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="5 6">
        <name>pMOL28</name>
    </geneLocation>
    <geneLocation type="plasmid" evidence="4 6">
        <name>pMOL30</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="submission" date="2006-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of the chromosome of Ralstonia metallidurans CH34.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Copeland A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Barry K."/>
                <person name="Detter J.C."/>
                <person name="Glavina del Rio T."/>
                <person name="Hammon N."/>
                <person name="Israni S."/>
                <person name="Dalin E."/>
                <person name="Tice H."/>
                <person name="Martinez M."/>
                <person name="Goltsman E."/>
                <person name="Pitluck S."/>
                <person name="Schmutz J."/>
                <person name="Larimer F."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Kim E."/>
                <person name="Mergeay M."/>
                <person name="Benotmane M.A."/>
                <person name="Vallaeys T."/>
                <person name="Michaux A."/>
                <person name="Monchy S."/>
                <person name="Dunn J."/>
                <person name="McCorkle S."/>
                <person name="Taghavi S."/>
                <person name="van der Lelie D."/>
                <person name="Richardson P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">CH34</strain>
            <plasmid evidence="5">pMOL28</plasmid>
            <plasmid evidence="4">pMOL30</plasmid>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="6">Plasmid pMOL28</plasmid>
            <plasmid evidence="6">Plasmid pMOL30</plasmid>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2010-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Benotmane R."/>
                <person name="Lapidus A."/>
                <person name="McCorkle S."/>
                <person name="Monchy S."/>
                <person name="Taghavi S."/>
                <person name="van der Lelie N."/>
                <person name="Dunn J."/>
                <person name="Leys N."/>
                <person name="Mergeay M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">CH34</strain>
            <plasmid evidence="5">pMOL28</plasmid>
            <plasmid evidence="4">pMOL30</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000354">
        <property type="protein sequence ID" value="ABF13034.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP000355">
        <property type="protein sequence ID" value="ABF13043.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_000995360.1">
        <property type="nucleotide sequence ID" value="NC_007972.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_145635.1">
        <property type="nucleotide sequence ID" value="NC_006466.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_161726.1">
        <property type="nucleotide sequence ID" value="NC_006525.1"/>
    </dbReference>
    <dbReference type="SMR" id="Q5NUV1"/>
    <dbReference type="EnsemblBacteria" id="ABF13034">
        <property type="protein sequence ID" value="ABF13034"/>
        <property type="gene ID" value="Rmet_6175"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF13043">
        <property type="protein sequence ID" value="ABF13043"/>
        <property type="gene ID" value="Rmet_6184"/>
    </dbReference>
    <dbReference type="GeneID" id="61089176"/>
    <dbReference type="KEGG" id="rme:Rmet_6175"/>
    <dbReference type="KEGG" id="rme:Rmet_6184"/>
    <dbReference type="HOGENOM" id="CLU_140237_0_0_4"/>
    <dbReference type="OMA" id="NELACCA"/>
    <dbReference type="OrthoDB" id="1748559at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid pMOL28"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid pMOL30"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045892">
        <property type="term" value="P:negative regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046689">
        <property type="term" value="P:response to mercury ion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009061">
        <property type="entry name" value="DNA-bd_dom_put_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011797">
        <property type="entry name" value="MerD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000551">
        <property type="entry name" value="MerR-type_HTH_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13411">
        <property type="entry name" value="MerR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00040">
        <property type="entry name" value="HTHMERR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00422">
        <property type="entry name" value="HTH_MERR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46955">
        <property type="entry name" value="SSF46955"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02054">
        <property type="entry name" value="MerD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50937">
        <property type="entry name" value="HTH_MERR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0614">Plasmid</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH merR-type" evidence="3">
        <location>
            <begin position="3"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00019396"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00032882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF13034.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABF13043.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="121" mass="13018" checksum="FEB2C162D7FFBC2C" modified="2005-01-04" version="1">MNAYTVSRLALDAGVSVHIVRDYLLRGLLRPVACTPGGYGLFDDAALQRLCFVRAAFEAGIGLDALARLCRALDAADGDEAAAQLALLRQFVERRREALADLEVQLATLPTEPAQHAESLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-03-23" modified="2020-10-07" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>D3DXM6</accession>
    <name>D3DXM6_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_6429</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ADC45046.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_6429"/>
    <dbReference type="EnsemblBacteria" id="ADC45046">
        <property type="protein sequence ID" value="ADC45046"/>
        <property type="gene ID" value="Rmet_6429"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6429"/>
    <dbReference type="HOGENOM" id="CLU_2619474_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="12"/>
            <end position="38"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADC45046.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="78" mass="9073" checksum="8608F0D2DD6AFB44" modified="2010-03-23" version="1">MLWIIVGNLAKKSNNRKTPGKARRPAPRKDRGQADRLKNQLKTEAEKRGYLATVPANEFFTSDRLARRIREQIRSRIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-03-23" modified="2021-06-02" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>D3DXN9</accession>
    <name>D3DXN9_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_6445</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ADC45059.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADC45059">
        <property type="protein sequence ID" value="ADC45059"/>
        <property type="gene ID" value="Rmet_6445"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6445"/>
    <dbReference type="HOGENOM" id="CLU_2481051_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="37"/>
            <end position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADC45059.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="87" mass="8816" checksum="77518ABB7977AE47" modified="2010-03-23" version="1">MKPEAQPRHRQDSQRDKDALGRTAPKGDGAGSASPHEGFSGSPTDAGQVNHPTTGENAPAAPCGAALEAWRGSSIAMPGGLSVNRPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-03-23" modified="2020-10-07" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>D3DXS4</accession>
    <name>D3DXS4_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_6486</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ADC45094.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_6486"/>
    <dbReference type="EnsemblBacteria" id="ADC45094">
        <property type="protein sequence ID" value="ADC45094"/>
        <property type="gene ID" value="Rmet_6486"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6486"/>
    <dbReference type="HOGENOM" id="CLU_2587190_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADC45094.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="80" mass="8645" checksum="78832556B9C59ECA" modified="2010-03-23" version="1">MLGVAWARDEMLRRVARAMLRVLVMALGPWSERLALDGVWLTYMDARSGCLGVGVADGPPIGRFLGSIRSWMVGSAHAGC</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-03-23" modified="2020-10-07" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>D3DY11</accession>
    <name>D3DY11_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_6580</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ADC45181.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADC45181">
        <property type="protein sequence ID" value="ADC45181"/>
        <property type="gene ID" value="Rmet_6580"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6580"/>
    <dbReference type="HOGENOM" id="CLU_3414942_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADC45181.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="27" mass="2988" checksum="2BB5DD7B0D8BF87B" modified="2010-03-23" version="1">MQPHGLGKVGAKIRKKHIADHGQNNPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-03-23" modified="2020-10-07" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>D3DYC2</accession>
    <name>D3DYC2_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_6712</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ADC45292.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_6712"/>
    <dbReference type="EnsemblBacteria" id="ADC45292">
        <property type="protein sequence ID" value="ADC45292"/>
        <property type="gene ID" value="Rmet_6712"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6712"/>
    <dbReference type="HOGENOM" id="CLU_2976093_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="31"/>
            <end position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADC45292.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="58" mass="6516" checksum="26C48090B5BE2533" modified="2010-03-23" version="1">MLAIYFSSFALLVLSQLFEWHHRRSETPSRALYWVSAFSYSTSLLVMVCAGATIVIGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-03-23" modified="2021-06-02" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>D3DYH9</accession>
    <name>D3DYH9_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_6352</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2 3">
        <name>pMOL30</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid pMOL30</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000354">
        <property type="protein sequence ID" value="ADC45349.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011514786.1">
        <property type="nucleotide sequence ID" value="NC_007971.2"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADC45349">
        <property type="protein sequence ID" value="ADC45349"/>
        <property type="gene ID" value="Rmet_6352"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6352"/>
    <dbReference type="HOGENOM" id="CLU_2957396_0_0_4"/>
    <dbReference type="OrthoDB" id="2231892at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid pMOL30"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="34"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="35"/>
            <end position="59"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADC45349.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="59" mass="6807" checksum="28037FC3DC8ED459" modified="2010-03-23" version="1">MTKTSFRSEFVTAAREAPRLFFAPLVGAIQAVRKEARRTPTEQNKEKQDSLHGFRKQRS</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1990-08-01" modified="2022-05-25" version="103" xmlns="http://uniprot.org/uniprot">
    <accession>P17552</accession>
    <accession>Q1L9W4</accession>
    <accession>Q5NV01</accession>
    <accession>Q93JN1</accession>
    <name>CHRB1_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Protein ChrB</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">chrB1</name>
        <name evidence="3" type="synonym">chrB</name>
        <name evidence="5" type="ordered locus">Rmet_6203</name>
        <name evidence="7" type="ORF">RMe0089</name>
    </gene>
    <organism>
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid">
        <name>pMOL28</name>
    </geneLocation>
    <reference key="1">
        <citation type="journal article" date="1990" name="J. Biol. Chem." volume="265" first="5648" last="5653">
            <title>Nucleotide sequence and expression of a plasmid-encoded chromate resistance determinant from Alcaligenes eutrophus.</title>
            <authorList>
                <person name="Nies A."/>
                <person name="Nies D.H."/>
                <person name="Silver S."/>
            </authorList>
            <dbReference type="PubMed" id="2180932"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC DNA]</scope>
        <scope>FUNCTION</scope>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2002" name="Arch. Microbiol." volume="179" first="15" last="25">
            <title>New genes involved in chromate resistance in Ralstonia metallidurans strain CH34.</title>
            <authorList>
                <person name="Juhnke S."/>
                <person name="Peitzsch N."/>
                <person name="Hubener N."/>
                <person name="Grosse C."/>
                <person name="Nies D.H."/>
            </authorList>
            <dbReference type="PubMed" id="12471500"/>
            <dbReference type="DOI" id="10.1007/s00203-002-0492-5"/>
        </citation>
        <scope>SEQUENCE REVISION TO C-TERMINUS</scope>
        <scope>INDUCTION</scope>
    </reference>
    <reference key="3">
        <citation type="submission" date="2004-10" db="EMBL/GenBank/DDBJ databases">
            <title>Sequence and features of the Ralstonia metallidurans CH34 heavy metal plasmids pMOL28 and pMOL30.</title>
            <authorList>
                <person name="van der Lelie D."/>
                <person name="Monchy S."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="Benotmane M."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Mergeay M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference key="4">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Together with ChrA1, this protein reduces chromate accumulation and is essential for chromate resistance, possibly as a regulatory protein.</text>
    </comment>
    <comment type="induction">
        <text evidence="1">By chromate; induction increases when cells are grown in the presence of high sulfate concentrations (3 mM SO4(2-)).</text>
    </comment>
    <comment type="sequence caution" evidence="4">
        <conflict type="erroneous initiation">
            <sequence resource="EMBL-CDS" id="CAI30233" version="1"/>
        </conflict>
        <text>Truncated N-terminus.</text>
    </comment>
    <dbReference type="EMBL" id="AJ313327">
        <property type="protein sequence ID" value="CAC42410.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="X90708">
        <property type="protein sequence ID" value="CAI30233.1"/>
        <property type="status" value="ALT_INIT"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP000355">
        <property type="protein sequence ID" value="ABF13062.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A35177">
        <property type="entry name" value="A35177"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011514878.1">
        <property type="nucleotide sequence ID" value="NC_007972.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_161711.1">
        <property type="nucleotide sequence ID" value="NC_006525.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="P17552"/>
    <dbReference type="SMR" id="P17552"/>
    <dbReference type="EnsemblBacteria" id="ABF13062">
        <property type="protein sequence ID" value="ABF13062"/>
        <property type="gene ID" value="Rmet_6203"/>
    </dbReference>
    <dbReference type="GeneID" id="60825780"/>
    <dbReference type="KEGG" id="rme:Rmet_6203"/>
    <dbReference type="HOGENOM" id="CLU_079058_0_0_4"/>
    <dbReference type="OMA" id="FTHIGDR"/>
    <dbReference type="OrthoDB" id="1108707at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid pMOL28"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046687">
        <property type="term" value="P:response to chromate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018634">
        <property type="entry name" value="Chromate_resist"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09828">
        <property type="entry name" value="Chrome_Resist"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword id="KW-0155">Chromate resistance</keyword>
    <keyword id="KW-0614">Plasmid</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000089655" description="Protein ChrB">
        <location>
            <begin position="1"/>
            <end position="324"/>
        </location>
    </feature>
    <evidence type="ECO:0000269" key="1">
        <source>
            <dbReference type="PubMed" id="12471500"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="2">
        <source>
            <dbReference type="PubMed" id="2180932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000303" key="3">
        <source>
            <dbReference type="PubMed" id="2180932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="4"/>
    <evidence type="ECO:0000312" key="5">
        <source>
            <dbReference type="EMBL" id="ABF13062.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000312" key="6">
        <source>
            <dbReference type="EMBL" id="CAC42410.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000312" key="7">
        <source>
            <dbReference type="EMBL" id="CAI30233.1"/>
        </source>
    </evidence>
    <sequence length="324" mass="35993" checksum="F3B6922FD73A4675" modified="2002-05-27" version="2">MNALPSSPETAWLLLVVSLPTSASTARMRFWRGIKALGATALRDGAYLLPNLPGLRAPLQTLATDAASEDGKVWMLSVQAADDQQEAEYRALFDRSTEYAEWMVELSSARSTLSDSDEAELLRVARRHGRGIDAIRKVDFFPNEASARAELQWRDFNAAIDILLSPGEPHGVAGNIPRRDPTQYQGRQWATRQHLWVDRVACAWLIRRFIDPHATFLWLEDVRQCPDDALGFDFDGATFTHIGDRVSFEVLLASFGLDEDKGLARLGQMIHVLDVGGTPVAEASGFEAVLAGARERLPNDDALLDEVGYVLDSLYTHFSSPRKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-10-07" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LA66</accession>
    <name>Q1LA66_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_6101</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="1 2">
        <name>pMOL30</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid pMOL30</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000354">
        <property type="protein sequence ID" value="ABF12960.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF12960">
        <property type="protein sequence ID" value="ABF12960"/>
        <property type="gene ID" value="Rmet_6101"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6101"/>
    <dbReference type="HOGENOM" id="CLU_1815062_0_0_4"/>
    <dbReference type="OMA" id="IFERIWA"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid pMOL30"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF12960.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="143" mass="16381" checksum="01493C26D98D6D4E" modified="2006-05-30" version="1">MVAETQSPSSCAGRRGRLGERAVTDPSYHTRKQPFSVVSLTASSLWDQSNMATLDLIGVAIQFKDWEKPWTFYSVVLDHISHDSDDRAIFERIWAEACRPEHWQYVDISKCSDACDHGLEIGFSWLPEEARVQFVRAASFQWK</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LA78</accession>
    <name>Q1LA78_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_6089</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="1 2">
        <name>pMOL30</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid pMOL30</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000354">
        <property type="protein sequence ID" value="ABF12948.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011514854.1">
        <property type="nucleotide sequence ID" value="NC_007971.2"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF12948">
        <property type="protein sequence ID" value="ABF12948"/>
        <property type="gene ID" value="Rmet_6089"/>
    </dbReference>
    <dbReference type="GeneID" id="60825031"/>
    <dbReference type="KEGG" id="rme:Rmet_6089"/>
    <dbReference type="HOGENOM" id="CLU_679472_0_0_4"/>
    <dbReference type="OrthoDB" id="172711at28216"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid pMOL30"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF12948.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="405" mass="43914" checksum="42FBCDA9F2B36D55" modified="2006-05-30" version="1">MPTVPIHKEFRLLSSTPNLTWQKKTPPASGAHVNIWWLYLTMQRASKLGLTLGGSGQETVRATAEAALSVCAWDGHGYSVTPAYSRLKDYVKPFLSGTLGAGLALLQMDHAGYPWMAHWEDCGGQTTSAHPDFVFFRAATATTPAAVCLAESKGSRATTTDLDVQKDWERQVWSNRHQVLTLPGGGNVAPTEGTLVSSELPGPAGKKRFRSTLVHGVFPSGLPAVTPATASSPGGGVGPNAAGGNQPLDQHAPEMDWRGIQRASLRHTCRLLDMPLTESILASRFWDGFSLNLLFTQPRPSARRVVLELRDRRALLGWDDQSEPDVVYAGPPQVVVQPDGSWWGATIYCRASVLREVLRGRVVDVVDTTERRVRSWGEGGQQMIRLEAADGVGLMVRKMFGAPGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-10-07" version="62" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LAN4</accession>
    <name>Q1LAN4_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_5933</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12792.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF12792">
        <property type="protein sequence ID" value="ABF12792"/>
        <property type="gene ID" value="Rmet_5933"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5933"/>
    <dbReference type="eggNOG" id="ENOG502ZQ5C">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_077139_0_0_4"/>
    <dbReference type="OMA" id="WRMRAIN"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021409">
        <property type="entry name" value="DUF3047"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11249">
        <property type="entry name" value="DUF3047"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193243" evidence="1">
        <location>
            <begin position="30"/>
            <end position="247"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF12792.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="247" mass="26604" checksum="826BA75250590046" modified="2006-05-30" version="1">MPSILTKARLLGLAACLLPTVLHCVPVRAQTVEPGVVFSTARPGSGLPSGWQNRPVLPGKKMTSYVLVADQGTTVLEADASGSASGLVHEGDFDLNKTPVVSWRWKVAAPIQNADNRVADREDSPARLVFFFGGQVDSLSIGDRAEMFAAHALGESLPYATLMYIWTNSAAPGTVIENPHSSRVQMIVVGNEAGKWQSLRRNVVADFEKVFHEKPGKLTGYGILTDTDNTGGVARAWYGDIRFSPQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="102" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LAX3</accession>
    <name>Q1LAX3_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Protein-tyrosine kinase</fullName>
            <ecNumber evidence="11">2.7.10.2</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">epsB1</name>
        <name evidence="11" type="ordered locus">Rmet_5844</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="12">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="12">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EC" id="2.7.10.2" evidence="11"/>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12703.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5844"/>
    <dbReference type="EnsemblBacteria" id="ABF12703">
        <property type="protein sequence ID" value="ABF12703"/>
        <property type="gene ID" value="Rmet_5844"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5844"/>
    <dbReference type="eggNOG" id="COG0489">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG3206">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009912_0_0_4"/>
    <dbReference type="OMA" id="NSMEYQR"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004715">
        <property type="term" value="F:non-membrane spanning protein tyrosine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045226">
        <property type="term" value="P:extracellular polysaccharide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025669">
        <property type="entry name" value="AAA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005702">
        <property type="entry name" value="EPS_synthesis"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032807">
        <property type="entry name" value="GNVR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13614">
        <property type="entry name" value="AAA_31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13807">
        <property type="entry name" value="GNVR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01007">
        <property type="entry name" value="eps_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3 11" id="KW-0418">Kinase</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-0614">Plasmid</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 11" id="KW-0808">Transferase</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5 11" id="KW-0829">Tyrosine-protein kinase</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="32"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="domain" description="GNVR" evidence="10">
        <location>
            <begin position="376"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="domain" description="AAA_31" evidence="9">
        <location>
            <begin position="567"/>
            <end position="697"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="276"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="348"/>
            <end position="406"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF13614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF13807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ABF12703.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="780" mass="83724" checksum="61C12B9F1588190C" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LAY7</accession>
    <name>Q1LAY7_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF1653 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_5830</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12689.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5830"/>
    <dbReference type="EnsemblBacteria" id="ABF12689">
        <property type="protein sequence ID" value="ABF12689"/>
        <property type="gene ID" value="Rmet_5830"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5830"/>
    <dbReference type="eggNOG" id="COG4339">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG4728">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_059733_0_0_4"/>
    <dbReference type="OMA" id="MPYRHYK"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.320">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023387">
        <property type="entry name" value="DUF1653-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037135">
        <property type="entry name" value="DUF1653-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009218">
        <property type="entry name" value="HD_phosphohydro"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21174">
        <property type="entry name" value="PTHR21174"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07866">
        <property type="entry name" value="DUF1653"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF1653" evidence="2">
        <location>
            <begin position="122"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="81"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="83"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07866"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF12689.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="389" mass="42165" checksum="191C616B5B186DC0" modified="2006-05-30" version="1">MVRRSGFLRRRTQGAIAGSVATICAHSNGRVNLRCAAHKSPPATTASDAAVTPGFKPEAHNGGLDSRTRFARHALRCSDSITTGGPLAATENTGSQSQPQSQSPADLTALPDNPDLVPGMPYRHYKGGAYLVTGVGRFEADMTPVVVYESMRDRSLLWVRHADVFTEPVSTATGDVARFAPHWAPALSCLDFLPRKTVLQVLALYDAPYRKYHDRRHVLEMFEQAHALGIALNPAQALAVLFHDAVYVPGFEHNEAASAALLDTMVEDVNADVIHLARQIILDTRTHEASVPDAATVLDLDLLRLAADDDVFDAHGLAVFDENRGLLASRVQLSGEALYHAFMVRRAAFLGALAQRPKLFLTTAFAEFEGPARRNIARVLASFGQAKAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="103" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LB01</accession>
    <name>Q1LB01_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Major cold shock protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">cspA</name>
        <name evidence="4" type="ordered locus">Rmet_5816</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="5">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="5">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12675.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008646365.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5816"/>
    <dbReference type="EnsemblBacteria" id="ABF12675">
        <property type="protein sequence ID" value="ABF12675"/>
        <property type="gene ID" value="Rmet_5816"/>
    </dbReference>
    <dbReference type="GeneID" id="60823560"/>
    <dbReference type="KEGG" id="rme:Rmet_5816"/>
    <dbReference type="eggNOG" id="COG1278">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_117621_0_3_4"/>
    <dbReference type="OMA" id="DGQKVQF"/>
    <dbReference type="OrthoDB" id="2034748at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04458">
        <property type="entry name" value="CSP_CDS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012156">
        <property type="entry name" value="Cold_shock_CspA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011129">
        <property type="entry name" value="CSD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019844">
        <property type="entry name" value="CSD_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002059">
        <property type="entry name" value="CSP_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00313">
        <property type="entry name" value="CSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002599">
        <property type="entry name" value="Cold_shock_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00050">
        <property type="entry name" value="COLDSHOCK"/>
    </dbReference>
    <dbReference type="SMART" id="SM00357">
        <property type="entry name" value="CSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00352">
        <property type="entry name" value="CSD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51857">
        <property type="entry name" value="CSD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0614">Plasmid</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CSD" evidence="3">
        <location>
            <begin position="1"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU000408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF12675.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="67" mass="7342" checksum="D33960920685F5CB" modified="2006-05-30" version="1">MQTGIVKWFNDAKGFGFIKPDAGGDDLFAHFSEVRADGFKSLQENQRVSFEVKNGPKGLQAANITPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LB17</accession>
    <name>Q1LB17_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 7">6-phosphogluconolactonase</fullName>
            <shortName evidence="7">6PGL</shortName>
            <ecNumber evidence="5 7">3.1.1.31</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">pgl</name>
        <name evidence="9" type="ordered locus">Rmet_5800</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="10">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="10">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2 7">Hydrolysis of 6-phosphogluconolactone to 6-phosphogluconate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 7">
            <text>6-phospho-D-glucono-1,5-lactone + H2O = 6-phospho-D-gluconate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:12556"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57955"/>
            <dbReference type="ChEBI" id="CHEBI:58759"/>
            <dbReference type="EC" id="3.1.1.31"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3 7">Carbohydrate degradation; pentose phosphate pathway; D-ribulose 5-phosphate from D-glucose 6-phosphate (oxidative stage): step 2/3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 7">Belongs to the glucosamine/galactosamine-6-phosphate isomerase family. 6-phosphogluconolactonase subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.31" evidence="5 7"/>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12659.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011520195.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5800"/>
    <dbReference type="EnsemblBacteria" id="ABF12659">
        <property type="protein sequence ID" value="ABF12659"/>
        <property type="gene ID" value="Rmet_5800"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5800"/>
    <dbReference type="eggNOG" id="COG0363">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_053947_2_1_4"/>
    <dbReference type="OMA" id="YQLFEFE"/>
    <dbReference type="OrthoDB" id="1828393at2"/>
    <dbReference type="UniPathway" id="UPA00115">
        <property type="reaction ID" value="UER00409"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017057">
        <property type="term" value="F:6-phosphogluconolactonase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006098">
        <property type="term" value="P:pentose-phosphate shunt"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd01400">
        <property type="entry name" value="6PGL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005900">
        <property type="entry name" value="6-phosphogluconolactonase_DevB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006148">
        <property type="entry name" value="Glc/Gal-6P_isomerase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037171">
        <property type="entry name" value="NagB/RpiA_transferase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039104">
        <property type="entry name" value="PGLS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11054">
        <property type="entry name" value="PTHR11054"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01182">
        <property type="entry name" value="Glucosamine_iso"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100950">
        <property type="entry name" value="SSF100950"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01198">
        <property type="entry name" value="pgl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0614">Plasmid</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glucosamine_iso" evidence="8">
        <location>
            <begin position="13"/>
            <end position="215"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002681"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010662"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00013198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00020337"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU365095"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ABF12659.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="226" mass="24203" checksum="656428305D852D4E" modified="2006-05-30" version="1">MRQFEHATLMDQAESLAISIGHALDLAIKAKGWAVLAVSGGKSPQAMFERLRYRPVRWEAVTVTLVDERAVPPDDADSNGAMVRSHLLREGAGVAAWAPLIRDEAEAAHPQSAVARLNQAFLQPDVVVLGMGEDGHTASLFPDAPELQTALSEPTPGYIVTHPSVAPHARVTLNLAALLAADRMFLAISGEKKAAVLEQALKTPTPSLPVSVVLARHRHGVDVFRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-10-07" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LB53</accession>
    <name>Q1LB53_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_5764</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12623.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF12623">
        <property type="protein sequence ID" value="ABF12623"/>
        <property type="gene ID" value="Rmet_5764"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5764"/>
    <dbReference type="HOGENOM" id="CLU_1766489_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="40"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="63"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="121"/>
            <end position="147"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF12623.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="147" mass="15314" checksum="887837D088F501A7" modified="2006-05-30" version="1">MLIWLSGSHARAGCSGAVRPWWSPAFPYQDGTLMFTGSIHWLRGVAIVVLLGAASDMAFAASQAKSLDTQRGIRDGSSGGTELSTAPLSQRGVTGTQSMGSTDGSGQYPYIVAPYIQVGPGGGPIPYPPNPPHPPQPPRPRPTPHAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-04-07" version="105" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LBB0</accession>
    <name>Q1LBB0_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Two component transcriptional regulator, winged helix family (Receiver domain)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Rmet_5707</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="7">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="7">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12566.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5707"/>
    <dbReference type="EnsemblBacteria" id="ABF12566">
        <property type="protein sequence ID" value="ABF12566"/>
        <property type="gene ID" value="Rmet_5707"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5707"/>
    <dbReference type="eggNOG" id="COG0745">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_30_0_4"/>
    <dbReference type="OMA" id="AHACPET"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001867">
        <property type="entry name" value="OmpR/PhoB-type_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016032">
        <property type="entry name" value="Sig_transdc_resp-reg_C-effctor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039420">
        <property type="entry name" value="WalR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48111">
        <property type="entry name" value="PTHR48111"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00486">
        <property type="entry name" value="Trans_reg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00862">
        <property type="entry name" value="Trans_reg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46894">
        <property type="entry name" value="SSF46894"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51755">
        <property type="entry name" value="OMPR_PHOB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0614">Plasmid</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Response regulatory" evidence="4">
        <location>
            <begin position="45"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="domain" description="OmpR/PhoB-type" evidence="5">
        <location>
            <begin position="171"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="OmpR/PhoB-type" evidence="3">
        <location>
            <begin position="171"/>
            <end position="270"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABF12566.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="277" mass="30612" checksum="9135D70A4B96C994" modified="2006-05-30" version="1">MDHSFHRKFPNRPAEWLCYSQSIQHECAPIAGAPGFRKLKVNSIKIALLDAEPSSATGISKVLEAGGVVSSRFTSSERLFRELRRSVFDVVLVSDNLPDDAATDVVQALRRATTFQMPILIIAHHASQVNLIDAFDAGADDYVMFASDPNSLLPRIIGHHRRARRRGGSGGRQLSAGDYTLDLDACCVRFRAETIRLTPKEFALAKLMFSSPAVTLPRDHIEIVVWGSTLPPLSRALAAMVARLRRVLRLRPEHGVTLRGVHSMGYRLDIAAPESRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="59" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LBC9</accession>
    <name>Q1LBC9_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_5688</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12547.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011520089.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5688"/>
    <dbReference type="EnsemblBacteria" id="ABF12547">
        <property type="protein sequence ID" value="ABF12547"/>
        <property type="gene ID" value="Rmet_5688"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5688"/>
    <dbReference type="eggNOG" id="COG4726">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_126486_0_0_4"/>
    <dbReference type="OMA" id="QWDVAAT"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF12547.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="181" mass="18778" checksum="5EFB2A435A3EC7CA" modified="2006-05-30" version="1">MKMHRTRRRQSGATLLITMIFVVIFLLLVISLVSTGIVNTKVTANQQHNVEAVSAAQQGIEQVISQDFTSAPVATTVPVDVNGDGKADYTAQVATPVCQSSTTITNTQLDVNNPDDVACFVGNGNNNTGIIDATGGSGGNSLCNTTQWDVSATVSDAAATNANATLHQGIAVRVPYGTACP</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LBF4</accession>
    <name>Q1LBF4_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 6">Phosphate-binding protein PstS</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">pstS</name>
        <name evidence="10" type="ordered locus">Rmet_5663</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="11">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="11">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 6">Part of the ABC transporter complex PstSACB involved in phosphate import.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3 6">The complex is composed of two ATP-binding proteins (PstB), two transmembrane proteins (PstC and PstA) and a solute-binding protein (PstS).</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the PstS family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12522.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011520067.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5663"/>
    <dbReference type="EnsemblBacteria" id="ABF12522">
        <property type="protein sequence ID" value="ABF12522"/>
        <property type="gene ID" value="Rmet_5663"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5663"/>
    <dbReference type="eggNOG" id="COG0226">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_034528_1_0_4"/>
    <dbReference type="OMA" id="QFVYAYI"/>
    <dbReference type="OrthoDB" id="1222928at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043190">
        <property type="term" value="C:ATP-binding cassette (ABC) transporter complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042301">
        <property type="term" value="F:phosphate ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035435">
        <property type="term" value="P:phosphate ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005673">
        <property type="entry name" value="ABC_phos-bd_PstS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024370">
        <property type="entry name" value="PBP_domain"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12849">
        <property type="entry name" value="PBP_like_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002756">
        <property type="entry name" value="PstS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00975">
        <property type="entry name" value="3a0107s03"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0592">Phosphate transport</keyword>
    <keyword evidence="10" id="KW-0614">Plasmid</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193272" description="Phosphate-binding protein PstS" evidence="8">
        <location>
            <begin position="22"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="domain" description="PBP_domain" evidence="9">
        <location>
            <begin position="19"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="region of interest" description="Phosphate binding" evidence="7">
        <location>
            <begin position="29"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="region of interest" description="Phosphate binding" evidence="7">
        <location>
            <begin position="159"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="58"/>
        </location>
        <ligand>
            <name>phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="76"/>
        </location>
        <ligand>
            <name>phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002841"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00021889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR002756"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR002756-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF12849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ABF12522.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="355" mass="37128" checksum="6A42F03F02FA6348" modified="2006-05-30" version="1">MLKRTALAILGSALIMSTAVAADIQGAGSTAAAPVYRVWSTGYAAATGTRLNYAAVGSGEGIRRIREGQVDFGASDVPLSPQEASKSGLVCVPAVVTAAVPVINLPSVPKGQLKLTGDLLAKIFLGKVDSWDAPEIRAVNPGLALPNLKIHPVVRADGSGTTYHFSAYLSEVSAEWKSTYGTKSAFSWPAGYTGAKGSGEVVKAVQSNRGAIGYVDYNYVLDEGLNPVQLRNADGNFVSATVGGFRDAVVHSNWNRKGDFTASLVNQAGADAWPITMGTFVVVPAVSRAGERTLEAMKFVTWGYLHGDQLAREAKFVPLPGQVQANAYRELARVTDASGKAIGLQSMASPAGKAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="60" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LBN4</accession>
    <name>Q1LBN4_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_5583</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12442.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008649522.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5583"/>
    <dbReference type="EnsemblBacteria" id="ABF12442">
        <property type="protein sequence ID" value="ABF12442"/>
        <property type="gene ID" value="Rmet_5583"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5583"/>
    <dbReference type="HOGENOM" id="CLU_140204_1_0_4"/>
    <dbReference type="OMA" id="TMDYNPQ"/>
    <dbReference type="OrthoDB" id="1955080at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193043" evidence="2">
        <location>
            <begin position="33"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="28"/>
            <end position="52"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF12442.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="113" mass="11731" checksum="7F20DE93BB14AEAE" modified="2006-05-30" version="1">MTRSNRQLKRLTTASAVALVAMVAGCASQPPAEPIPNPGPQGGRGTPEGCQADKLGDEALIGKTEAEAAALLKGCAWRFGSRDGQSLPATMDYNPQRRSIDVVGGKVTAVRRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LC25</accession>
    <name>Q1LC25_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Glycerol-3-phosphate responsive antiterminator (MRNA-binding)-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_5442</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12301.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519848.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5442"/>
    <dbReference type="EnsemblBacteria" id="ABF12301">
        <property type="protein sequence ID" value="ABF12301"/>
        <property type="gene ID" value="Rmet_5442"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5442"/>
    <dbReference type="eggNOG" id="COG1954">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_111516_0_0_4"/>
    <dbReference type="OMA" id="PAIRNMK"/>
    <dbReference type="OrthoDB" id="1560657at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006071">
        <property type="term" value="P:glycerol metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006699">
        <property type="entry name" value="GlpP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35787">
        <property type="entry name" value="PTHR35787"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04309">
        <property type="entry name" value="G3P_antiterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016897">
        <property type="entry name" value="GlpP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF12301.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="196" mass="21241" checksum="C2B595484E4CFAEB" modified="2006-05-30" version="1">MDRSLGARLTRHPVIATLYGADQIDAFIDSEAEVSIVANVELRRLQPVIATLTKAGKYVIVNIDSCEGLSQDKGGVEYLADIGVTSLVSTRVATIQRANRAGMMTMQKVFVTDRSTWPRSVKALEQSDANLVQLMPAPMLQHLPGSVRKGLPPIVASGFVSTQDDIRSAIAHDALAVSTSDQRLWACDPQAKTIRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LC52</accession>
    <name>Q1LC52_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Putative alkyl sulfatase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Rmet_5415</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="8">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="8">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the metallo-beta-lactamase superfamily. Type III sulfatase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12274.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519822.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5415"/>
    <dbReference type="EnsemblBacteria" id="ABF12274">
        <property type="protein sequence ID" value="ABF12274"/>
        <property type="gene ID" value="Rmet_5415"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5415"/>
    <dbReference type="eggNOG" id="COG2015">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014655_1_0_4"/>
    <dbReference type="OMA" id="ANTPTGM"/>
    <dbReference type="OrthoDB" id="775108at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018741">
        <property type="term" value="F:alkyl sulfatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018909">
        <property type="term" value="P:dodecyl sulfate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07710">
        <property type="entry name" value="arylsulfatase_Sdsa1-like_MBL-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1050.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038536">
        <property type="entry name" value="Alkyl/aryl-sulf_dimr_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029229">
        <property type="entry name" value="Alkyl_sulf_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029228">
        <property type="entry name" value="Alkyl_sulf_dimr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044097">
        <property type="entry name" value="Bds1/SdsA1_MBL-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001279">
        <property type="entry name" value="Metallo-B-lactamas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036527">
        <property type="entry name" value="SCP2_sterol-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14864">
        <property type="entry name" value="Alkyl_sulf_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14863">
        <property type="entry name" value="Alkyl_sulf_dimr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00753">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00849">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55718">
        <property type="entry name" value="SSF55718"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="SSF56281"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0614">Plasmid</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193294" evidence="5">
        <location>
            <begin position="27"/>
            <end position="662"/>
        </location>
    </feature>
    <feature type="domain" description="Lactamase_B" evidence="6">
        <location>
            <begin position="133"/>
            <end position="355"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00033751"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABF12274.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="662" mass="73253" checksum="149F3C9B1CE3F17A" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LCG3</accession>
    <name>Q1LCG3_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Metallophosphoesterase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Rmet_5304</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="5">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="5">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12163.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5304"/>
    <dbReference type="EnsemblBacteria" id="ABF12163">
        <property type="protein sequence ID" value="ABF12163"/>
        <property type="gene ID" value="Rmet_5304"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5304"/>
    <dbReference type="eggNOG" id="COG1409">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_040518_0_0_4"/>
    <dbReference type="OMA" id="MSIFTNH"/>
    <dbReference type="OrthoDB" id="550557at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP_ApaH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="SSF56300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0614">Plasmid</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193059" evidence="2">
        <location>
            <begin position="21"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="domain" description="Metallophos" evidence="3">
        <location>
            <begin position="165"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="30"/>
            <end position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF12163.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="509" mass="52660" checksum="1B06081989C395FA" modified="2006-05-30" version="1">MRTKKALGWTAGMVALVAVAGLHGCGSDDTNANSSNNGSGNGGTTTPPTVTPADAATASANIQAAWVEIGDSNQAIARAVTSYVAPADAGANGVCPMLTVDGKATRMTLRVGPATVPLRTTASAPADSKPSEFPVSVCEATVASTATSAAIGTQTLPLPKALPQRIVVLADTGCRLKKADNAWQACSDTDAWPLSTVATTAAGFNPDLVLHIGDYHYRENACPPDIAGCQGSPWGYGWDTWQADLFKPAAPLLAKAPWVVVRGNHEECARAGQGWSRFLDPRPFDTTRSCDDPVNDSTGNYADPYAVSLGGGSQVIVFDSAKAGKAALPTTDPQFIAYQKQFQTVATLAAKPGMTTTIFTNHHPILGFAPIAGANPAPGNLALQSVMSNLNAQAYYPTGIHVALHGHVHDFQAINFASAHPATIVTGNGGDNLDVALPDPLPAGSVPAPGTTIDRITHHASFGFLVMDRKASPATGWTFKAYAKDGKLMATCNQTGNTVTCDKTGFIAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LCS8</accession>
    <name>Q1LCS8_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">VOC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_5189</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12048.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519595.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5189"/>
    <dbReference type="EnsemblBacteria" id="ABF12048">
        <property type="protein sequence ID" value="ABF12048"/>
        <property type="gene ID" value="Rmet_5189"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5189"/>
    <dbReference type="eggNOG" id="COG0346">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046006_6_1_4"/>
    <dbReference type="OMA" id="LWPEYHP"/>
    <dbReference type="OrthoDB" id="1857145at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.180.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029068">
        <property type="entry name" value="Glyas_Bleomycin-R_OHBP_Dase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004360">
        <property type="entry name" value="Glyas_Fos-R_dOase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037523">
        <property type="entry name" value="VOC"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00903">
        <property type="entry name" value="Glyoxalase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54593">
        <property type="entry name" value="SSF54593"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51819">
        <property type="entry name" value="VOC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="VOC" evidence="1">
        <location>
            <begin position="1"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF12048.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="128" mass="13544" checksum="8CC1F14D58AF3196" modified="2006-05-30" version="1">MIDHTGVNVSDFSRSLDFYAAALGAIGIVKIMEIPASVTGHTDVAGFGPPGKPEFWIISGAPNKPPLHVAFRVDSRAEVDAFYKAAMAAGGRDNGGPGLRPHYHPDYYGAFVLDPDGHNIEAVCHQAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LCX2</accession>
    <name>Q1LCX2_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Putative enoyl-CoA hydratase/isomerase</fullName>
            <ecNumber evidence="4">4.2.1.17</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Rmet_5145</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="5">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="5">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the enoyl-CoA hydratase/isomerase family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.17" evidence="4"/>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12004.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519551.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5145"/>
    <dbReference type="EnsemblBacteria" id="ABF12004">
        <property type="protein sequence ID" value="ABF12004"/>
        <property type="gene ID" value="Rmet_5145"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5145"/>
    <dbReference type="eggNOG" id="COG1024">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009834_7_2_4"/>
    <dbReference type="OMA" id="TMAYTRV"/>
    <dbReference type="OrthoDB" id="1275789at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004300">
        <property type="term" value="F:enoyl-CoA hydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016853">
        <property type="term" value="F:isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.12.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029045">
        <property type="entry name" value="ClpP/crotonase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018376">
        <property type="entry name" value="Enoyl-CoA_hyd/isom_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001753">
        <property type="entry name" value="Enoyl-CoA_hydra/iso"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014748">
        <property type="entry name" value="Enoyl-CoA_hydra_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00378">
        <property type="entry name" value="ECH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52096">
        <property type="entry name" value="SSF52096"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00166">
        <property type="entry name" value="ENOYL_COA_HYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0413">Isomerase</keyword>
    <keyword evidence="4" id="KW-0456">Lyase</keyword>
    <keyword evidence="4" id="KW-0614">Plasmid</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF12004.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="260" mass="28332" checksum="98776451A6E1D29F" modified="2006-05-30" version="1">MTVLEVRHVGTTAVVTMNRPEQRNALDMTMRDAFAEVVPALRDDKAVKAIVLTGAGGHFCAGGDVKAIAAGQGGERDIFEGRERIRKIHRWSDELIDMEKPVIAAVDGVAFGAGLSLALCADFILASKRATFCSVFSRIGFVPDVGALYLLPRWIGLARAKELVFSARVVKADEALDLGLLHSISDDDVVADAIALANRFAHAPTDAIGAAKSVMNRAFESDRHTVHAQEAMLQAMCRESAYHQEAVRRFIDKEPAKYQW</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LD49</accession>
    <name>Q1LD49_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_5065</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2011-04" db="PDB data bank">
            <title>Solution NMR structure of protein Rmet_5065 from Ralstonia metallidurans. Northeast Structural Genomics Consortium Target CrR115.</title>
            <authorList>
                <person name="Ramelot T.A."/>
                <person name="Yang Y."/>
                <person name="Cort J.R."/>
                <person name="Wang D."/>
                <person name="Ciccosanti C."/>
                <person name="Janjua H."/>
                <person name="Rajesh N."/>
                <person name="Acton T.B."/>
                <person name="Xiao R."/>
                <person name="Everett J.K."/>
                <person name="Montelione G.T."/>
                <person name="Kennedy M.A."/>
            </authorList>
        </citation>
        <scope>STRUCTURE BY NMR</scope>
    </reference>
    <reference evidence="5 6" key="3">
        <citation type="journal article" date="2015" name="Nat. Methods" volume="12" first="751" last="754">
            <title>Protein structure determination by combining sparse NMR data with evolutionary couplings.</title>
            <authorList>
                <person name="Tang Y."/>
                <person name="Huang Y.J."/>
                <person name="Hopf T.A."/>
                <person name="Sander C."/>
                <person name="Marks D.S."/>
                <person name="Montelione G.T."/>
            </authorList>
            <dbReference type="PubMed" id="26121406"/>
            <dbReference type="DOI" id="10.1038/nmeth.3455"/>
        </citation>
        <scope>STRUCTURE BY NMR</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the AHA1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11927.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519477.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="PDB" id="2LCG">
        <property type="method" value="NMR"/>
        <property type="chains" value="A=1-134"/>
    </dbReference>
    <dbReference type="PDB" id="2N4A">
        <property type="method" value="NMR"/>
        <property type="chains" value="A=1-134"/>
    </dbReference>
    <dbReference type="PDB" id="2N4B">
        <property type="method" value="NMR"/>
        <property type="chains" value="A=1-134"/>
    </dbReference>
    <dbReference type="PDBsum" id="2LCG"/>
    <dbReference type="PDBsum" id="2N4A"/>
    <dbReference type="PDBsum" id="2N4B"/>
    <dbReference type="SMR" id="Q1LD49"/>
    <dbReference type="STRING" id="266264.Rmet_5065"/>
    <dbReference type="EnsemblBacteria" id="ABF11927">
        <property type="protein sequence ID" value="ABF11927"/>
        <property type="gene ID" value="Rmet_5065"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5065"/>
    <dbReference type="eggNOG" id="COG3832">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_108923_1_0_4"/>
    <dbReference type="OMA" id="ENDLRVG"/>
    <dbReference type="OrthoDB" id="1914312at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.530.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013538">
        <property type="entry name" value="Activator_of_Hsp90_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023393">
        <property type="entry name" value="START-like_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08327">
        <property type="entry name" value="AHSA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword evidence="4 5 6" id="KW-0002">3D-structure</keyword>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006817"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF11927.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="4">
        <source>
            <dbReference type="PDB" id="2LCG"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="5">
        <source>
            <dbReference type="PDB" id="2N4A"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="6">
        <source>
            <dbReference type="PDB" id="2N4B"/>
        </source>
    </evidence>
    <sequence length="134" mass="15049" checksum="CB0F9BA763EF7947" modified="2006-05-30" version="1">MNITVETTVAAPVGKVWRAYTTPEDIKQWNAASDDWHTTAATVDLREGGAFSSRMEAKDGSMGFDFAGTYTKVVENKRIEYAFGDRTAKVEFLEAPQGVTVRVSFVAETEYPVEQQQQGWQAILNNFKRHVESH</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="104" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LD86</accession>
    <name>Q1LD86_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">3-oxoacyl-(Acyl-carrier-protein) reductase</fullName>
            <ecNumber evidence="1">1.1.1.100</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">fabG</name>
        <name evidence="1" type="ordered locus">Rmet_5028</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EC" id="1.1.1.100" evidence="1"/>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11890.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519439.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5028"/>
    <dbReference type="EnsemblBacteria" id="ABF11890">
        <property type="protein sequence ID" value="ABF11890"/>
        <property type="gene ID" value="Rmet_5028"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5028"/>
    <dbReference type="eggNOG" id="COG1028">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010194_1_3_4"/>
    <dbReference type="OMA" id="NGGQLIC"/>
    <dbReference type="OrthoDB" id="1601931at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004316">
        <property type="term" value="F:3-oxoacyl-[acyl-carrier-protein] reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00061">
        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF11890.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="274" mass="29077" checksum="AAE4BEB66A13C9F9" modified="2006-05-30" version="1">MGALEGKVALVTGSGRGIGHAIAQRLAREGARLVINDLDAEPAHATVEALRKMGVEAVACVGNVTAPDFADRFIGTAMSEFKGIDIIVNNAGFTWDDVVQKMSDEQWHAILDCHMTAPFRILRAAYPHIKALHAADKEAGREVYRKIVNISSTSALNGNAGQINYSGAKAGVIGMTRAIAREWGRFNVNVNAVAFGLIHTRMTSADANAGATVNIEGREIRVGLNPEMLKSHAQRNPLGRGGTPEEAAGGVYLFCSPDSNYITGQTIAVAGNLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="99" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LDK0</accession>
    <name>Q1LDK0_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Putative ATP binding component of ABC transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">nosF</name>
        <name evidence="9" type="ordered locus">Rmet_4914</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="10">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="10">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11776.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519335.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4914"/>
    <dbReference type="EnsemblBacteria" id="ABF11776">
        <property type="protein sequence ID" value="ABF11776"/>
        <property type="gene ID" value="Rmet_4914"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4914"/>
    <dbReference type="eggNOG" id="COG1131">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_2_4"/>
    <dbReference type="OMA" id="IMCAFLV"/>
    <dbReference type="OrthoDB" id="1833499at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="1" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-0614">Plasmid</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-1278">Translocase</keyword>
    <feature type="domain" description="ABC transporter" evidence="8">
        <location>
            <begin position="13"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="45"/>
            <end position="52"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ABF11776.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="316" mass="34343" checksum="623EA8AAEF2FA93C" modified="2006-05-30" version="1">MTEHIHVEGQAAIALRGVCKDYGAVRAVDDVDLEIGYGELFGLIGHNGAGKSTLFRMMLGLIAASEGDIRVAGASVGGAAFRAARRQIGYLPENLVLYDNLSGLETLRFFGRMKGAPLADCELLLARVGLAGAAEKPVRAYSKGMRQRLGFAQALLGSPRVVFLDEPTNGLDPAAIHDFYAMLQALREQGVTIVITSHILAELQQRIDRLAIMGNGRILAVGSVAELRRQLDLPVTLALRVERGARAALWQHFAPLRAQGIELENGRAEYELILRCRRSLKMQVLGALHPMGGRLLDLQIQEPTLEDVFFGLKEAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="69" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LDU1</accession>
    <name>Q1LDU1_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_4823</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11685.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519248.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4823"/>
    <dbReference type="EnsemblBacteria" id="ABF11685">
        <property type="protein sequence ID" value="ABF11685"/>
        <property type="gene ID" value="Rmet_4823"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4823"/>
    <dbReference type="eggNOG" id="COG2964">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_080179_0_1_4"/>
    <dbReference type="OMA" id="MYGEHTE"/>
    <dbReference type="OrthoDB" id="1474443at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039446">
        <property type="entry name" value="DauR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039445">
        <property type="entry name" value="DauR-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013559">
        <property type="entry name" value="YheO"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35568">
        <property type="entry name" value="PTHR35568"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13309">
        <property type="entry name" value="HTH_22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08348">
        <property type="entry name" value="PAS_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PAS_6" evidence="1">
        <location>
            <begin position="12"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="domain" description="HTH_22" evidence="2">
        <location>
            <begin position="147"/>
            <end position="208"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF08348"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13309"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF11685.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="208" mass="22539" checksum="8869F64A81B58546" modified="2006-05-30" version="1">MVKRTAEQRVLLDQIKQIADGLGQTLAPFCEVVVHDLLEPEQSILAIHNNLSGRAVGDPTTELGQARITDPDYPQVLANYANQFADGRQAKSTSIGIKDASGNYIAALCMNVDLTLFRSLQMAMGQFVQTATDGPKETLDPAGTDALRDRIDAFAARLATTPRALKTEERRALLGELKASGLLDVRRAMETVAAHLGVSRAAVYSYVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="102" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LDV9</accession>
    <name>Q1LDV9_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">2-amino-3-ketobutyrate coenzyme A ligase</fullName>
            <shortName evidence="1">AKB ligase</shortName>
            <ecNumber evidence="1">2.3.1.29</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Glycine acetyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1 3" type="primary">kbl</name>
        <name evidence="3" type="ordered locus">Rmet_4805</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the cleavage of 2-amino-3-ketobutyrate to glycine and acetyl-CoA.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>acetyl-CoA + glycine = (2S)-2-amino-3-oxobutanoate + CoA</text>
            <dbReference type="Rhea" id="RHEA:20736"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
            <dbReference type="ChEBI" id="CHEBI:78948"/>
            <dbReference type="EC" id="2.3.1.29"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
        <text evidence="1">Binds 1 pyridoxal phosphate per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid degradation; L-threonine degradation via oxydo-reductase pathway; glycine from L-threonine: step 2/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-II pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="1">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.29" evidence="1"/>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11667.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519232.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4805"/>
    <dbReference type="EnsemblBacteria" id="ABF11667">
        <property type="protein sequence ID" value="ABF11667"/>
        <property type="gene ID" value="Rmet_4805"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4805"/>
    <dbReference type="eggNOG" id="COG0156">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015846_11_0_4"/>
    <dbReference type="OMA" id="MDTHGFG"/>
    <dbReference type="OrthoDB" id="479874at2"/>
    <dbReference type="UniPathway" id="UPA00046">
        <property type="reaction ID" value="UER00506"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008890">
        <property type="term" value="F:glycine C-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009058">
        <property type="term" value="P:biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019518">
        <property type="term" value="P:L-threonine catabolic process to glycine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00985">
        <property type="entry name" value="2am3keto_CoA_ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011282">
        <property type="entry name" value="2am3keto_CoA_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001917">
        <property type="entry name" value="Aminotrans_II_pyridoxalP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="SSF53383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01822">
        <property type="entry name" value="2am3keto_CoA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00599">
        <property type="entry name" value="AA_TRANSFER_CLASS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 3" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="1" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aminotran_1_2" evidence="2">
        <location>
            <begin position="45"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="region of interest" description="Pyridoxal phosphate binding" evidence="1">
        <location>
            <begin position="243"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="region of interest" description="Pyridoxal phosphate binding; shared with dimeric partner" evidence="1">
        <location>
            <begin position="276"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="138"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="187"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="370"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="1">
        <location>
            <position position="246"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00985"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF11667.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="400" mass="43524" checksum="C0B806B0897E2FCC" modified="2006-05-30" version="1">MSSAEAFYASIRAELDSIRAAGLYKRERVIATPQGAKIRTADGREVINLCANNYLGLSSHPKVIEAAHEALRTHGYGLSSVRFICGTQDLHKELEGRLARFLGTEDTILYGSAFDANGGLFETILGPEDAVISDELNHASIIDGIRLSKARRLRYRHNDLDDLRAQLRQADADGARFKLVFSDGVFSMDGTVARLDEIRKICDEHGALLGIDECHATGFMGARGRGTHEARGVFGKIDIITGTLGKALGGASGGFTSGRKEVVELLRQRSRPYLFSNTVAPAIVGGSIAVLDILEGSTELRDRLEQNTQFFREGIAKLGFDIKPGDHPIVPIMVYDAEKAQQLADRLLELGVYVVGFFYPVVPKGQARIRVQMSAVHDAVTLQSALDAFGQAGRELGLIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="70" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LE80</accession>
    <name>Q1LE80_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">UPF0276 protein Rmet_4684</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_4684</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0276 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11546.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519118.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="SMR" id="Q1LE80"/>
    <dbReference type="STRING" id="266264.Rmet_4684"/>
    <dbReference type="EnsemblBacteria" id="ABF11546">
        <property type="protein sequence ID" value="ABF11546"/>
        <property type="gene ID" value="Rmet_4684"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4684"/>
    <dbReference type="eggNOG" id="COG3220">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_064263_0_0_4"/>
    <dbReference type="OMA" id="EWDTDVP"/>
    <dbReference type="OrthoDB" id="1602134at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00697">
        <property type="entry name" value="UPF0276"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007801">
        <property type="entry name" value="UPF0276"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036237">
        <property type="entry name" value="Xyl_isomerase-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42194">
        <property type="entry name" value="PTHR42194"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05114">
        <property type="entry name" value="DUF692"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51658">
        <property type="entry name" value="SSF51658"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF11546.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="278" mass="31062" checksum="E44C0DB4A3F0F067" modified="2006-05-30" version="1">MTATPRAGLGLKPQHFEQALQCAEPGLWFEVHPENYLMAGGPRLAWLTAIRERHPVALHGVSLSLAGDAPPDEAHLDRLHALLRRIEPVLVSEHLAWSTLDGRYVPDLLPVARTTARLAKVAANISRVQDRLGCAIALENPSHYLRMDEHDWSETDFLAELVRRTGCGLLLDINNVYVSARNMGYDAWDYLTRVPAEAVREIHLAGHSFDDEFGASLLIDSHDAPVAEAVWSLYRRFVARVGERPTLIERDDNIPEFADLLDERDRAQSILDAEVATS</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="98" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LEF2</accession>
    <name>Q1LEF2_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Transcriptional regulator, IclR family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Rmet_4612</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="7">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="7">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11474.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519049.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4612"/>
    <dbReference type="EnsemblBacteria" id="ABF11474">
        <property type="protein sequence ID" value="ABF11474"/>
        <property type="gene ID" value="Rmet_4612"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4612"/>
    <dbReference type="eggNOG" id="COG1414">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_062618_4_0_4"/>
    <dbReference type="OMA" id="RRITSAW"/>
    <dbReference type="OrthoDB" id="370329at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029016">
        <property type="entry name" value="GAF-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014757">
        <property type="entry name" value="Tscrpt_reg_IclR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005471">
        <property type="entry name" value="Tscrpt_reg_IclR_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09339">
        <property type="entry name" value="HTH_IclR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01614">
        <property type="entry name" value="IclR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00346">
        <property type="entry name" value="HTH_ICLR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51077">
        <property type="entry name" value="HTH_ICLR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51078">
        <property type="entry name" value="ICLR_ED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0614">Plasmid</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH iclR-type" evidence="4">
        <location>
            <begin position="5"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="domain" description="IclR-ED" evidence="5">
        <location>
            <begin position="66"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="253"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="262"/>
            <end position="279"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABF11474.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="285" mass="30435" checksum="BE63A031E55359DE" modified="2006-05-30" version="1">MATRSSPVQRALAVLRALSDPEVRRMSDVARATGLDPATTSRLLDLLVQEGFATRDARRHYGIGPEVFQLADVGQRRVDLRQLARPALERLQDAFGDTAVLTETSRGEAVCSEVITGTYPVHANYVTVGTRRPLGVGAGSLAVLAWLPPAERARRLQQTARKLANYPQLDAASIAAEADAACARGYVLFLDWVVEGMGGIAVPILDYDGWPIGALSVAALSTRIRSREAAMARLLRQEARRITSAWTRAVVPAGGTQRAGPRSRGASASGISTSKRSTALAKALP</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LEG2</accession>
    <name>Q1LEG2_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Putative DnaK suppressor protein, dksA-like (Tn6050)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Rmet_4470</name>
        <name evidence="7" type="ordered locus">Rmet_4602</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="7">
        <name>megaplasmid</name>
    </geneLocation>
    <geneLocation type="plasmid" evidence="8">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="7" key="1">
        <citation type="submission" date="2006-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of the chromosome of Ralstonia metallidurans CH34.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Copeland A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Barry K."/>
                <person name="Detter J.C."/>
                <person name="Glavina del Rio T."/>
                <person name="Hammon N."/>
                <person name="Israni S."/>
                <person name="Dalin E."/>
                <person name="Tice H."/>
                <person name="Martinez M."/>
                <person name="Goltsman E."/>
                <person name="Pitluck S."/>
                <person name="Schmutz J."/>
                <person name="Larimer F."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Kim E."/>
                <person name="Mergeay M."/>
                <person name="Benotmane M.A."/>
                <person name="Vallaeys T."/>
                <person name="Michaux A."/>
                <person name="Monchy S."/>
                <person name="Dunn J."/>
                <person name="McCorkle S."/>
                <person name="Taghavi S."/>
                <person name="van der Lelie D."/>
                <person name="Richardson P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">CH34</strain>
            <plasmid evidence="7">megaplasmid</plasmid>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="8">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2010-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Benotmane R."/>
                <person name="Lapidus A."/>
                <person name="McCorkle S."/>
                <person name="Monchy S."/>
                <person name="Taghavi S."/>
                <person name="van der Lelie N."/>
                <person name="Dunn J."/>
                <person name="Leys N."/>
                <person name="Mergeay M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">CH34</strain>
            <plasmid evidence="7">megaplasmid</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11335.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11464.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518936.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4470"/>
    <dbReference type="EnsemblBacteria" id="ABF11335">
        <property type="protein sequence ID" value="ABF11335"/>
        <property type="gene ID" value="Rmet_4470"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF11464">
        <property type="protein sequence ID" value="ABF11464"/>
        <property type="gene ID" value="Rmet_4602"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4470"/>
    <dbReference type="KEGG" id="rme:Rmet_4602"/>
    <dbReference type="eggNOG" id="COG1734">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_043144_3_3_4"/>
    <dbReference type="OMA" id="ECGESIG"/>
    <dbReference type="OrthoDB" id="1932026at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000962">
        <property type="entry name" value="Znf_DskA_TraR"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51128">
        <property type="entry name" value="ZF_DKSA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0614">Plasmid</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="DksA C4-type" evidence="5">
        <location>
            <begin position="39"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="dksA C4-type" evidence="4">
        <location>
            <begin position="82"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00510"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABF11335.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABF11464.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="111" mass="12344" checksum="4DA4BE58212AE7C8" modified="2006-05-30" version="1">MDMEQFRARLLIEQRETVEAIQQAQQSAAPVELDQSCVGRVSRIDALQQQALAQGLRERLTIRKRKVEAALARLDSGTYGLCCACHSDLEPELLNADPAVVFCQECATARQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LEP1</accession>
    <name>Q1LEP1_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Universal stress protein UspA family UspA14</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">uspA14</name>
        <name evidence="3" type="ordered locus">Rmet_4520</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal stress protein A family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11385.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518978.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4520"/>
    <dbReference type="EnsemblBacteria" id="ABF11385">
        <property type="protein sequence ID" value="ABF11385"/>
        <property type="gene ID" value="Rmet_4520"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4520"/>
    <dbReference type="eggNOG" id="COG0589">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_049301_5_2_4"/>
    <dbReference type="OMA" id="CEARIRY"/>
    <dbReference type="OrthoDB" id="2040681at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006015">
        <property type="entry name" value="Universal_stress_UspA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006016">
        <property type="entry name" value="UspA"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00582">
        <property type="entry name" value="Usp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01438">
        <property type="entry name" value="UNVRSLSTRESS"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Usp" evidence="2">
        <location>
            <begin position="209"/>
            <end position="272"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008791"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF11385.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="274" mass="28731" checksum="E3A0CD9EAAA7D991" modified="2006-05-30" version="1">MDFKTILVDLAADPARDARLDAALTLASLFTARVVGLTATGTLLDPFRSAGEETARYQQMRADMLRNLKQADAAALDAAVARRGRGIEISHVVVEQEAGWALTSRGVSSDLILPAPPSLAQDTPALMATSAEYVLLNSGRPILVIPPGGSLVPGGTVVVAWDGRREAARAMRDALPLLALASRVILQVVLSGAGPNEHGAAEAVRWLADHGVSASLRIDEAASPADSLMRVTRNEAADLLVTGGYGHSRLGELVMGGTTRTLLRNAPVPLLMSH</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LEQ1</accession>
    <name>Q1LEQ1_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Membrane fusion protein of efflux pump HlyD family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_4510</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11375.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518968.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4510"/>
    <dbReference type="EnsemblBacteria" id="ABF11375">
        <property type="protein sequence ID" value="ABF11375"/>
        <property type="gene ID" value="Rmet_4510"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4510"/>
    <dbReference type="eggNOG" id="COG1566">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018816_15_2_4"/>
    <dbReference type="OMA" id="QRIPMRV"/>
    <dbReference type="OrthoDB" id="757027at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039562">
        <property type="entry name" value="MFP_biotin_lipoyl_2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13533">
        <property type="entry name" value="Biotin_lipoyl_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Biotin_lipoyl_2" evidence="1">
        <location>
            <begin position="50"/>
            <end position="97"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF11375.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="349" mass="37428" checksum="C0F401DD9DBAD2D9" modified="2006-05-30" version="1">MKMAGVQKGPLRGRVITLAIVLVAVLLSVYAYERTVHHPSSDDATLDADVVHVASPVGGRIARIAVTENQHVAKGDVLFEIDPVPYKLAVDQARADLELAQAGLGTRRRTIVSERAAASIASEQTTRAAQNYALTARTVERLRPLAADGYVPKQQLDQAQVAQHDAAVSLKQANVQHSASTQAIGNDDDAIAAVHAREAALAIAQRALQDTVVHAPQEGRVTGLSVLAGEVVIPNQSLFLLVHTGEWFAVANFRETSLADIHIGDCATVYSMIDRRKAIRGTVTGIGAGITDTERLNLPRSLPYVQASVNWVRVAKRFPVRIRLEDPPETLARAGASANVEIRHGAACR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LF27</accession>
    <name>Q1LF27_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Protein with predicted thioesterase domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_4384</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11249.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518866.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4384"/>
    <dbReference type="EnsemblBacteria" id="ABF11249">
        <property type="protein sequence ID" value="ABF11249"/>
        <property type="gene ID" value="Rmet_4384"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4384"/>
    <dbReference type="eggNOG" id="COG0824">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_101141_3_0_4"/>
    <dbReference type="OMA" id="WHGHYVK"/>
    <dbReference type="OrthoDB" id="1786865at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029069">
        <property type="entry name" value="HotDog_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54637">
        <property type="entry name" value="SSF54637"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF11249.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="138" mass="16091" checksum="317634D82A3DFF5C" modified="2006-05-30" version="1">MPRILSHEITLTPAFHDLDPMNVVWHGNYLRYFELARCALLQQIDYDYPQMSVSGYAWPIVDVRVKYVRPLRYGQPVIVRATFAEWENRLRIDYEMRDALSGARLTKGYSIQVAVDMATQEMQYVSPPVLLQRLGVEP</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LF45</accession>
    <name>Q1LF45_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Major facilitator superfamily MFS_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Rmet_4366</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="8">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="8">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
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        <property type="protein sequence ID" value="ABF11231.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
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    <dbReference type="EnsemblBacteria" id="ABF11231">
        <property type="protein sequence ID" value="ABF11231"/>
        <property type="gene ID" value="Rmet_4366"/>
    </dbReference>
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    <dbReference type="KEGG" id="rme:Rmet_4366"/>
    <dbReference type="eggNOG" id="COG2223">
        <property type="taxonomic scope" value="Bacteria"/>
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    <dbReference type="OrthoDB" id="1594866at2"/>
    <dbReference type="Proteomes" id="UP000002429">
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    </dbReference>
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        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="term" value="F:oxalate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
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        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026355">
        <property type="entry name" value="Oxa/Form_antiport"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SSF103473"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="oxa_formateAnti"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0614">Plasmid</keyword>
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    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="23"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="63"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="90"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="115"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="147"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="180"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="230"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="262"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="306"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="330"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="366"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="392"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="domain" description="MFS" evidence="6">
        <location>
            <begin position="18"/>
            <end position="418"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABF11231.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="447" mass="47422" checksum="DE7C541D488D8633" modified="2006-05-30" version="1">MAHTNAAGPAVGNFNGGLLRNRWFQLFIGVICMGLVANLQYGWTLFVTPMNARHHWGESAIQVAFSIFIVTETWLVPLEGWLVDKFGPRPVVAGGAICAGLAWVMNSYATTLPMLYTAAVIAGIGAGGVYGTCVGNALKWFPDKRGLAAGLTAAGFGAGSALTVIPIANMIQKSGYEHAFFTFGILQGVLIFAMALLLVKPQPPKGVVANKAILTNPVEFTPGQMVRTPVFWVIYASFVAVAAGGIMATAQLGPIAKDFGFASMPVTMLGMTLPLLTMTLSIDNLCNGLTRPLCGFISDRIGRENTMFVIFIGEGLSLIGLLELGHDPYWFMLFAALTFLFWGEIFSIFPALCADTFGSKFAAANAGTLYTAKGTAALLVPLASVLSHHGGWNLVFTVAAVMTVAAGLSAKFVLQPMRRRFITGHAMAQPVSDHATYMAGFSTGKGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="92" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LF76</accession>
    <name>Q1LF76_CUPMC</name>
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        <submittedName>
            <fullName evidence="5">Fumarate reductase/succinate dehydrogenase flavoprotein-like protein, oxidoreductase (AsfA-like), putative L-aspartate oxidase</fullName>
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        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">asfA</name>
        <name evidence="5" type="ordered locus">Rmet_4335</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="6">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="6">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
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    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11200.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4335"/>
    <dbReference type="EnsemblBacteria" id="ABF11200">
        <property type="protein sequence ID" value="ABF11200"/>
        <property type="gene ID" value="Rmet_4335"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4335"/>
    <dbReference type="eggNOG" id="COG1053">
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    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014312_8_5_4"/>
    <dbReference type="OMA" id="YMRLMRK"/>
    <dbReference type="OrthoDB" id="153138at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008734">
        <property type="term" value="F:L-aspartate oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044318">
        <property type="term" value="F:L-aspartate:fumarate oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008152">
        <property type="term" value="P:metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003953">
        <property type="entry name" value="FAD-binding_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037099">
        <property type="entry name" value="Fum_R/Succ_DH_flav-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015939">
        <property type="entry name" value="Fum_Rdtase/Succ_DH_flav-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030664">
        <property type="entry name" value="SdhA/FrdA/AprA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11632">
        <property type="entry name" value="PTHR11632"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SSF46977"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SSF51905"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="2 5" id="KW-0560">Oxidoreductase</keyword>
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    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="20"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="domain" description="Succ_DH_flav_C" evidence="4">
        <location>
            <begin position="457"/>
            <end position="506"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00890"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF02910"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABF11200.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="549" mass="58757" checksum="45AE4BF1A1D3F30F" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="60" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LFT4</accession>
    <name>Q1LFT4_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_4125</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10992.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518618.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4125"/>
    <dbReference type="EnsemblBacteria" id="ABF10992">
        <property type="protein sequence ID" value="ABF10992"/>
        <property type="gene ID" value="Rmet_4125"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4125"/>
    <dbReference type="eggNOG" id="ENOG50305GB">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_112398_1_0_4"/>
    <dbReference type="OMA" id="MTLGYDP"/>
    <dbReference type="OrthoDB" id="1961159at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011473">
        <property type="entry name" value="DUF1579"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07617">
        <property type="entry name" value="DUF1579"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF10992.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="165" mass="18586" checksum="80AA8546444DBB84" modified="2006-05-30" version="1">MKIEATNEHRWLQKLVGRWVGEGEAQMGPEAPPEKWKIPEQVSQVGDVWVQARAEGEFPGCGSAVTIMTLGYDPVRKHFVGTFIGSMMTHMWVYEGDLEADGRTLTLRADGPAFGPDGNVIEGKTAKYRDVVAMVDDDHRTLTSFMQADNGEWMQIVQARYRREK</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LFU9</accession>
    <name>Q1LFU9_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Transcriptional regulator, Xre/Cro/CI family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_4110</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10977.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518605.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4110"/>
    <dbReference type="EnsemblBacteria" id="ABF10977">
        <property type="protein sequence ID" value="ABF10977"/>
        <property type="gene ID" value="Rmet_4110"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4110"/>
    <dbReference type="eggNOG" id="COG0662">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG1396">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_085376_3_2_4"/>
    <dbReference type="OMA" id="TGMTKSY"/>
    <dbReference type="OrthoDB" id="1292067at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00093">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001387">
        <property type="entry name" value="Cro/C1-type_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013096">
        <property type="entry name" value="Cupin_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010982">
        <property type="entry name" value="Lambda_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011051">
        <property type="entry name" value="RmlC_Cupin_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07883">
        <property type="entry name" value="Cupin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01381">
        <property type="entry name" value="HTH_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00530">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47413">
        <property type="entry name" value="SSF47413"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51182">
        <property type="entry name" value="SSF51182"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50943">
        <property type="entry name" value="HTH_CROC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH cro/C1-type" evidence="2">
        <location>
            <begin position="34"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF10977.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="212" mass="23324" checksum="F06963D2D9C1A2A6" modified="2006-05-30" version="1">MDAPVQARTNLDSGMSKKQKVEPVAAEPSIAERLAELRKLNGLTLEELAQRASLTKSYLSKLERGLSSPTIGTVLKLADALGVTVDQLITRSSRANEILHIRAADRIPFSPSTERLGYTYEAIATERPDKAMQPFIMVPPFTLEEDQPMASHAGEELIFVVSGEMEVLFDDRTVRMSAGDSLYFNASIPHRSRSLGRIQAQALVVVSDRKKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="52" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LG49</accession>
    <name>Q1LG49_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_4009</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10877.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518516.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4009"/>
    <dbReference type="EnsemblBacteria" id="ABF10877">
        <property type="protein sequence ID" value="ABF10877"/>
        <property type="gene ID" value="Rmet_4009"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4009"/>
    <dbReference type="HOGENOM" id="CLU_1990831_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF10877.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="125" mass="13362" checksum="EDD8C556457F0793" modified="2006-05-30" version="1">MATLAAPAIPRAGVFSFAAPLLPTRADSIALYQTSNLSAQTDTTFHPAMVHDGDLFNDSARRSTMEMTHHPDTQCLNRATDGQCPVEAQDQPSNPAVAQSRSLAALILAIPPGDASDEWDFARVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="63" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LG60</accession>
    <name>Q1LG60_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_3998</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10866.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518505.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3998"/>
    <dbReference type="EnsemblBacteria" id="ABF10866">
        <property type="protein sequence ID" value="ABF10866"/>
        <property type="gene ID" value="Rmet_3998"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3998"/>
    <dbReference type="eggNOG" id="COG3209">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_074047_0_0_4"/>
    <dbReference type="OMA" id="WRTLDKP"/>
    <dbReference type="OrthoDB" id="1085497at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193150" evidence="1">
        <location>
            <begin position="28"/>
            <end position="236"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF10866.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="236" mass="25496" checksum="51A784328AF7190F" modified="2006-05-30" version="1">MPVSLLRRALTIGVMLATCGMTSNTLATTASRGTIALVMQSNGPNADVDQNIRIRLEGRGFTVRMLNQDTPASNAGDVRLVILSSTISAKQVSAGWRELAVPLLTWENDLLDDLAMTGKRLDTDYGESRRERFVWLVNAPHPVAAGLPAGATNVYRKQAAMNWGKPGLGASIIATLYGQPEKAVIFTYEKGATMDYESLAPARRVMFFLGNDTFTNLSGAGIKLFDAAIDWATGDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="69" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LGG7</accession>
    <name>Q1LGG7_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative endoribonuclease L-PSP</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_3891</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10759.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3891"/>
    <dbReference type="EnsemblBacteria" id="ABF10759">
        <property type="protein sequence ID" value="ABF10759"/>
        <property type="gene ID" value="Rmet_3891"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3891"/>
    <dbReference type="eggNOG" id="COG0251">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_104845_0_1_4"/>
    <dbReference type="OMA" id="GFDRLHH"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="CDD" id="cd02199">
        <property type="entry name" value="YjgF_YER057c_UK114_like_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013813">
        <property type="entry name" value="Endoribo_LPSP/chorism_mut-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035959">
        <property type="entry name" value="RutC-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14588">
        <property type="entry name" value="YjgF_endoribonc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55298">
        <property type="entry name" value="SSF55298"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="YjgF_endoribonc" evidence="1">
        <location>
            <begin position="4"/>
            <end position="149"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF14588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF10759.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="152" mass="16026" checksum="A12821A83AC163F1" modified="2006-05-30" version="1">MLQRLAARGLMLPDPPRPLGNYTAVSQAGDLLFVSGQLPLANGKVVWQGQVGRDLTLEEGQRAAVLAALNVLAQIRAYLGGFDRLDHIVRVEGHIASAEGWLQQPAVIDGASDLLVDVLGARAGHARTISSHFQQPGNAAVILVVIAQIGAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LGH0</accession>
    <name>Q1LGH0_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Major facilitator superfamily MFS_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Rmet_3888</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="8">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="8">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10756.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518403.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3888"/>
    <dbReference type="EnsemblBacteria" id="ABF10756">
        <property type="protein sequence ID" value="ABF10756"/>
        <property type="gene ID" value="Rmet_3888"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3888"/>
    <dbReference type="eggNOG" id="COG2271">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001265_5_1_4"/>
    <dbReference type="OMA" id="CVAWGGW"/>
    <dbReference type="OrthoDB" id="1345431at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000849">
        <property type="entry name" value="Sugar_P_transporter"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002808">
        <property type="entry name" value="Hexose_phosphate_transp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="SSF103473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0614">Plasmid</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="18"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="56"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="148"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="175"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="243"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="281"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="313"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="340"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="372"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="404"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="domain" description="MFS" evidence="6">
        <location>
            <begin position="22"/>
            <end position="430"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABF10756.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="433" mass="46618" checksum="DF920F11555D5B90" modified="2006-05-30" version="1">MQSTSPAAASLAVPRSSVRWKIFLMMLLLVAINYIDRASLSVAMPLIAKEFDLSPAMQGLILSSFFWTYAVMQVPGGMLADKYKPRIVIATATVFWGAFQALAAVCTNATSLLLTRLGLGAAEAPIYPAGGKLNAIWMTKNERGRGATLLDGGAPLGAALGSLIIAGLISELGSWRLSFVVAGVGTVLAGFVAWWYIRNSPREHLGVNEQEARHIEDALALEHSEEPTNLSGRSLDFFKYRSVWCMAISWMCFNAVFYGLLTWMPTYLSKARGFDLKQMGGASFIIFFAGFVGELIGGWIADKWKEAGGRPNVVMRTLFGIAAVVATVSIFSVAYVTDPVAVVVLLSSTLFFLRWCGLFWSVPSMLGTRNKVGFLGGVMNLGGNIAGISVPIIVGLIVQATGSYFLALMFFAACGIGLLLASTNIDYEKKIPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="106" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LHC7</accession>
    <name>Q1LHC7_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Phosphoenolpyruvate carboxykinase [GTP]</fullName>
            <shortName evidence="9">PEP carboxykinase</shortName>
            <shortName evidence="9">PEPCK</shortName>
            <ecNumber evidence="9">4.1.1.32</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">GTP-dependent phosphoenolpyruvate carboxykinase</fullName>
            <shortName evidence="9">GTP-PEPCK</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9 12" type="primary">pckG</name>
        <name evidence="12" type="ordered locus">Rmet_3577</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>GTP + oxaloacetate = CO2 + GDP + phosphoenolpyruvate</text>
            <dbReference type="Rhea" id="RHEA:10388"/>
            <dbReference type="ChEBI" id="CHEBI:16452"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
            <dbReference type="EC" id="4.1.1.32"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="9">Binds 1 Mn(2+) ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="9">Carbohydrate biosynthesis; gluconeogenesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 9">Belongs to the phosphoenolpyruvate carboxykinase [GTP] family.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.32" evidence="9"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10449.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517991.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3577"/>
    <dbReference type="EnsemblBacteria" id="ABF10449">
        <property type="protein sequence ID" value="ABF10449"/>
        <property type="gene ID" value="Rmet_3577"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3577"/>
    <dbReference type="eggNOG" id="COG1274">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028872_1_1_4"/>
    <dbReference type="OMA" id="GPTNNWV"/>
    <dbReference type="OrthoDB" id="267285at2"/>
    <dbReference type="UniPathway" id="UPA00138"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030145">
        <property type="term" value="F:manganese ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004613">
        <property type="term" value="F:phosphoenolpyruvate carboxykinase (GTP) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006094">
        <property type="term" value="P:gluconeogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00819">
        <property type="entry name" value="PEPCK_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.449.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.228.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00452">
        <property type="entry name" value="PEPCK_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018091">
        <property type="entry name" value="PEP_carboxykin_GTP_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013035">
        <property type="entry name" value="PEP_carboxykinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008209">
        <property type="entry name" value="PEP_carboxykinase_GTP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035077">
        <property type="entry name" value="PEP_carboxykinase_GTP_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035078">
        <property type="entry name" value="PEP_carboxykinase_GTP_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008210">
        <property type="entry name" value="PEP_carboxykinase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11561">
        <property type="entry name" value="PTHR11561"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00821">
        <property type="entry name" value="PEPCK_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17297">
        <property type="entry name" value="PEPCK_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001348">
        <property type="entry name" value="PEP_carboxykinase_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF68923">
        <property type="entry name" value="SSF68923"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00505">
        <property type="entry name" value="PEPCK_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 9" id="KW-0210">Decarboxylase</keyword>
    <keyword evidence="2 9" id="KW-0312">Gluconeogenesis</keyword>
    <keyword evidence="6 9" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="12" id="KW-0418">Kinase</keyword>
    <keyword evidence="8 9" id="KW-0456">Lyase</keyword>
    <keyword evidence="7 9" id="KW-0464">Manganese</keyword>
    <keyword evidence="3 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PEPCK_N" evidence="11">
        <location>
            <begin position="26"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="domain" description="PEPCK_GTP" evidence="10">
        <location>
            <begin position="249"/>
            <end position="615"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="9">
        <location>
            <begin position="224"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="9">
        <location>
            <begin position="396"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="active site" evidence="9">
        <location>
            <position position="277"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="85"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="233"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="253"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="275"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="276"/>
            <end position="281"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="302"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="398"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="429"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="526"/>
            <end position="529"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005796"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF17297"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ABF10449.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="618" mass="67885" checksum="DE3A8CDDA3865C4D" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="101" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LHG8</accession>
    <name>Q1LHG8_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Sensor protein QseC</fullName>
            <ecNumber evidence="4">2.7.13.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">phhS</name>
        <name evidence="13" type="ordered locus">Rmet_3536</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.</text>
            <dbReference type="EC" id="2.7.13.3"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell inner membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.7.13.3" evidence="4"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10408.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517954.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3536"/>
    <dbReference type="EnsemblBacteria" id="ABF10408">
        <property type="protein sequence ID" value="ABF10408"/>
        <property type="gene ID" value="Rmet_3536"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3536"/>
    <dbReference type="eggNOG" id="COG0642">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_89_37_4"/>
    <dbReference type="OMA" id="FDTGAEW"/>
    <dbReference type="OrthoDB" id="692375at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00082">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013727">
        <property type="entry name" value="2CSK_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003661">
        <property type="entry name" value="HisK_dim/P"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036097">
        <property type="entry name" value="HisK_dim/P_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004358">
        <property type="entry name" value="Sig_transdc_His_kin-like_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08521">
        <property type="entry name" value="2CSK_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00512">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00344">
        <property type="entry name" value="BCTRLSENSOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00388">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47384">
        <property type="entry name" value="SSF47384"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="SSF55874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="13" id="KW-0418">Kinase</keyword>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="43"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="206"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="12">
        <location>
            <begin position="290"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004429"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00017234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ABF10408.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="560" mass="60170" checksum="410B93355F9C4725" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="103" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LHG9</accession>
    <name>Q1LHG9_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Two component transcriptional regulator, winged helix family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">phhR</name>
        <name evidence="6" type="ordered locus">Rmet_3535</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10407.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008640981.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3535"/>
    <dbReference type="EnsemblBacteria" id="ABF10407">
        <property type="protein sequence ID" value="ABF10407"/>
        <property type="gene ID" value="Rmet_3535"/>
    </dbReference>
    <dbReference type="GeneID" id="60822640"/>
    <dbReference type="KEGG" id="rme:Rmet_3535"/>
    <dbReference type="eggNOG" id="COG0745">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_30_1_4"/>
    <dbReference type="OMA" id="IMITARV"/>
    <dbReference type="OrthoDB" id="1020672at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00383">
        <property type="entry name" value="trans_reg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001867">
        <property type="entry name" value="OmpR/PhoB-type_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016032">
        <property type="entry name" value="Sig_transdc_resp-reg_C-effctor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039420">
        <property type="entry name" value="WalR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48111">
        <property type="entry name" value="PTHR48111"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00486">
        <property type="entry name" value="Trans_reg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00862">
        <property type="entry name" value="Trans_reg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46894">
        <property type="entry name" value="SSF46894"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51755">
        <property type="entry name" value="OMPR_PHOB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Response regulatory" evidence="4">
        <location>
            <begin position="2"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="domain" description="OmpR/PhoB-type" evidence="5">
        <location>
            <begin position="127"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="OmpR/PhoB-type" evidence="3">
        <location>
            <begin position="127"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="2">
        <location>
            <position position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABF10407.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="234" mass="26093" checksum="3034598D5C0EA5E6" modified="2006-05-30" version="1">MRILLIEDDRQIASGVEAGLSRAGHQVRVVHDGVYATDHLLREQHDLVILDLGLPGIDGMTLLARYRARNRTTPVIILTARDELEDKLSGLNAGADDYLVKPFALPELEARVRVLLRRSQHGEAAPERDVRLGRLRLSGNDRRMFVDGRPLELSPREFAVLELLLQRQGRVVSKAQLQDHLATFAHPAGEGGDTVGDTAIEVYVHRVRKKLEDGDVEIVTVRGFGYLLQIRAGH</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LHV1</accession>
    <name>Q1LHV1_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Proline/glycine betaine transporter: Permease of the major facilitator superfamily (MFS) MFS transporter, MHS family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">proP</name>
        <name evidence="7" type="ordered locus">Rmet_3403</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10275.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517838.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3403"/>
    <dbReference type="EnsemblBacteria" id="ABF10275">
        <property type="protein sequence ID" value="ABF10275"/>
        <property type="gene ID" value="Rmet_3403"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3403"/>
    <dbReference type="eggNOG" id="COG0477">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001265_39_0_4"/>
    <dbReference type="OMA" id="STAINYM"/>
    <dbReference type="OrthoDB" id="961781at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="SSF103473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00216">
        <property type="entry name" value="SUGAR_TRANSPORT_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00217">
        <property type="entry name" value="SUGAR_TRANSPORT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="44"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="82"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="115"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="216"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="273"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="306"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="339"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="370"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="402"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="435"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="domain" description="MFS" evidence="6">
        <location>
            <begin position="44"/>
            <end position="457"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABF10275.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="461" mass="49417" checksum="F118CE47E7FABA00" modified="2006-05-30" version="1">MTPSPLPEHALAVASKGSLNIIIRAMSVTATSANELSKATRRRAIIAATIGNGLEWFDFTVYSFFAVIIAKLFFPTGNDMTSFLLTVATFGVGFFMRPVGAIVLGVYADRVGRKAALTLTILMMALGTAIIGLAPTYDSIGLWAPALIVLARLIQGFSAGGEVGGATAFLIEHAPDEERGMYASWQQASQGISFMLGAAMGALVTNGLDQAQIDAWGWRIPFLFGLLIGPVGMYIRSHLEEPPEFEARQAERRASNVKFSPLSQVLRDHPREVLAGLGVTILWTVCTYVLVFYMPSYAKQQLGLPLGATFQSTAICGAIILVLCPLMGMLSDRVGRKRMLGVVALAIGVLAYPLFHWLNVSPTTATLLQVQIVLGILLAAFTGPAPAVLAEQFPTEVRSTGLSLAYNFAVTIFGGFAPLIVTWLIESTHNKLAPAYYVIAAAAISFVALVFMHDRTGKKLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LHV6</accession>
    <name>Q1LHV6_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Glyoxalase/bleomycin resistance protein/ring-cleaving dioxygenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_3398</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10270.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517833.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3398"/>
    <dbReference type="EnsemblBacteria" id="ABF10270">
        <property type="protein sequence ID" value="ABF10270"/>
        <property type="gene ID" value="Rmet_3398"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3398"/>
    <dbReference type="eggNOG" id="COG0346">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046006_4_0_4"/>
    <dbReference type="OMA" id="NVDHICL"/>
    <dbReference type="OrthoDB" id="1984437at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051213">
        <property type="term" value="F:dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.180.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029068">
        <property type="entry name" value="Glyas_Bleomycin-R_OHBP_Dase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004360">
        <property type="entry name" value="Glyas_Fos-R_dOase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037523">
        <property type="entry name" value="VOC"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00903">
        <property type="entry name" value="Glyoxalase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54593">
        <property type="entry name" value="SSF54593"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51819">
        <property type="entry name" value="VOC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="VOC" evidence="2">
        <location>
            <begin position="6"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="110"/>
            <end position="136"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF10270.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="136" mass="14671" checksum="2993975929404930" modified="2006-05-30" version="1">MLKIRGIDHLVLRTADVPRLVRFYVDVLGCTIEREQPAFGLTQLRAGGGLIDLVSLDGPLGQAGGAGPGPEGHNLDHFCLRIDPFDEQAIFRHLSIHHVDASPVEQRFGAEGRGPSIYVKDPDGNTVELKGPATPD</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LI77</accession>
    <name>Q1LI77_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Protein-methionine-sulfoxide reductase heme-binding subunit MsrQ</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Flavocytochrome MsrQ</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">yedZ</name>
        <name evidence="7" type="synonym">msrQ</name>
        <name evidence="9" type="ordered locus">Rmet_3277</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Part of the MsrPQ system that repairs oxidized periplasmic proteins containing methionine sulfoxide residues (Met-O), using respiratory chain electrons. Thus protects these proteins from oxidative-stress damage caused by reactive species of oxygen and chlorine generated by the host defense mechanisms. MsrPQ is essential for the maintenance of envelope integrity under bleach stress, rescuing a wide series of structurally unrelated periplasmic proteins from methionine oxidation. MsrQ provides electrons for reduction to the reductase catalytic subunit MsrP, using the quinone pool of the respiratory chain.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="7">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
        <text evidence="7">Binds 1 FMN per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="7">
            <name>heme b</name>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
        </cofactor>
        <text evidence="7">Binds 1 heme b (iron(II)-protoporphyrin IX) group per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Heterodimer of a catalytic subunit (MsrP) and a heme-binding subunit (MsrQ).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cell inner membrane</location>
            <topology evidence="7">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the MsrQ family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10149.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517751.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3277"/>
    <dbReference type="EnsemblBacteria" id="ABF10149">
        <property type="protein sequence ID" value="ABF10149"/>
        <property type="gene ID" value="Rmet_3277"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3277"/>
    <dbReference type="eggNOG" id="COG2717">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_080662_0_0_4"/>
    <dbReference type="OMA" id="LHFFWMR"/>
    <dbReference type="OrthoDB" id="1508607at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005887">
        <property type="term" value="C:integral component of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030091">
        <property type="term" value="P:protein repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01207">
        <property type="entry name" value="MsrQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013130">
        <property type="entry name" value="Fe3_Rdtase_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022837">
        <property type="entry name" value="MsrQ-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36964">
        <property type="entry name" value="PTHR36964"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01794">
        <property type="entry name" value="Ferric_reduct"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="7" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0249">Electron transport</keyword>
    <keyword evidence="7" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="7" id="KW-0288">FMN</keyword>
    <keyword evidence="7" id="KW-0349">Heme</keyword>
    <keyword evidence="5 7" id="KW-0408">Iron</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 7" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="30"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="68"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="99"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="137"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="169"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="199"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="domain" description="Ferric oxidoreductase" evidence="8">
        <location>
            <begin position="68"/>
            <end position="182"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01207"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01794"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ABF10149.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="224" mass="25574" checksum="85469CB56D12D905" modified="2006-05-30" version="1">MAERDLPASAAQASPRHGGVALLAPSRVRAVKVCAWLLALLPFARLMYLGATSQFGANPLEFVTRSTGTWTLVMLCVTLAVTPLRRITGWHWLIRSRRMFGLFTFFYGVQHFMLWLAVDRGFDVAYMIKDIGKRPFITVGFAAFVLMVPLAATSANAMVRWLGGRRWQLLHRAIYAIAVLAILHYWWHKAGKNDFQEVSIYAVVVFVLLALRVWWWQQRRRAVP</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="107" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LI80</accession>
    <name>Q1LI80_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Penicillin-binding protein 1A</fullName>
            <ecNumber evidence="7">2.4.1.129</ecNumber>
            <ecNumber evidence="6">3.4.16.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="27" type="primary">mrcA</name>
        <name evidence="27" type="ordered locus">Rmet_3274</name>
    </gene>
    <organism evidence="27 28">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="28" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="28">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>[GlcNAc-(1-&gt;4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)](n)-di-trans,octa-cis-undecaprenyl diphosphate + beta-D-GlcNAc-(1-&gt;4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-di-trans,octa-cis-undecaprenyl diphosphate = [GlcNAc-(1-&gt;4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)](n+1)-di-trans-octa-cis-undecaprenyl diphosphate + di-trans,octa-cis-undecaprenyl diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:23708"/>
            <dbReference type="Rhea" id="RHEA-COMP:9602"/>
            <dbReference type="Rhea" id="RHEA-COMP:9603"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:58405"/>
            <dbReference type="ChEBI" id="CHEBI:60033"/>
            <dbReference type="ChEBI" id="CHEBI:78435"/>
            <dbReference type="EC" id="2.4.1.129"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Cell wall biogenesis; peptidoglycan biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell inner membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">In the C-terminal section; belongs to the transpeptidase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">In the N-terminal section; belongs to the glycosyltransferase 51 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.129" evidence="7"/>
    <dbReference type="EC" id="3.4.16.4" evidence="6"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10146.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517747.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3274"/>
    <dbReference type="CAZy" id="GT51">
        <property type="family name" value="Glycosyltransferase Family 51"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF10146">
        <property type="protein sequence ID" value="ABF10146"/>
        <property type="gene ID" value="Rmet_3274"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3274"/>
    <dbReference type="eggNOG" id="COG5009">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006354_2_4_4"/>
    <dbReference type="OMA" id="TYYDPAG"/>
    <dbReference type="OrthoDB" id="652304at2"/>
    <dbReference type="UniPathway" id="UPA00219"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008658">
        <property type="term" value="F:penicillin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008955">
        <property type="term" value="F:peptidoglycan glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009002">
        <property type="term" value="F:serine-type D-Ala-D-Ala carboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009252">
        <property type="term" value="P:peptidoglycan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008360">
        <property type="term" value="P:regulation of cell shape"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046677">
        <property type="term" value="P:response to antibiotic"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3810.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.710.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012338">
        <property type="entry name" value="Beta-lactam/transpept-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001264">
        <property type="entry name" value="Glyco_trans_51"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023346">
        <property type="entry name" value="Lysozyme-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036950">
        <property type="entry name" value="PBP_transglycosylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031376">
        <property type="entry name" value="PCB_OB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001460">
        <property type="entry name" value="PCN-bd_Tpept"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17092">
        <property type="entry name" value="PCB_OB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00912">
        <property type="entry name" value="Transgly"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00905">
        <property type="entry name" value="Transpeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53955">
        <property type="entry name" value="SSF53955"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56601">
        <property type="entry name" value="SSF56601"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="18" id="KW-0046">Antibiotic resistance</keyword>
    <keyword evidence="9" id="KW-0121">Carboxypeptidase</keyword>
    <keyword evidence="14" id="KW-0133">Cell shape</keyword>
    <keyword evidence="20" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="11" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="13" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="23" id="KW-0472">Membrane</keyword>
    <keyword evidence="19" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="16" id="KW-0573">Peptidoglycan synthesis</keyword>
    <keyword evidence="10" id="KW-0645">Protease</keyword>
    <keyword evidence="28" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="15" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="11" id="KW-0808">Transferase</keyword>
    <keyword evidence="12 23" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="17 23" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="20"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="domain" description="Transgly" evidence="25">
        <location>
            <begin position="73"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="domain" description="PCB_OB" evidence="26">
        <location>
            <begin position="332"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="domain" description="Transpeptidase" evidence="24">
        <location>
            <begin position="441"/>
            <end position="703"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="22">
        <location>
            <begin position="751"/>
            <end position="781"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004752"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00007739"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00012555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00018638"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00023988"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="Pfam" id="PF00905"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="Pfam" id="PF00912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="26">
        <source>
            <dbReference type="Pfam" id="PF17092"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="EMBL" id="ABF10146.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="792" mass="86875" checksum="3711DF7422E94379" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-10-07" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LI81</accession>
    <name>Q1LI81_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_3273</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10145.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3273"/>
    <dbReference type="EnsemblBacteria" id="ABF10145">
        <property type="protein sequence ID" value="ABF10145"/>
        <property type="gene ID" value="Rmet_3273"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3273"/>
    <dbReference type="HOGENOM" id="CLU_2466758_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF10145.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="88" mass="9785" checksum="91EDF2E4371390A2" modified="2006-05-30" version="1">MAWELACPALQAIAMERARRAGNGSGGVECVARNTGKDRRIGAGQRYATKLLLSVGFWQRFGRVDLKKKAWLNRHIASALGFCKNRRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="72" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LIL9</accession>
    <name>Q1LIL9_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Cell division protein FtsL</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6 8" type="primary">ftsL</name>
        <name evidence="8" type="ordered locus">Rmet_3135</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Essential cell division protein. May link together the upstream cell division proteins, which are predominantly cytoplasmic, with the downstream cell division proteins, which are predominantly periplasmic.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Part of a complex composed of FtsB, FtsL and FtsQ.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cell inner membrane</location>
            <topology evidence="6">Single-pass type II membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
        <text evidence="6">Localizes to the division septum where it forms a ring structure.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the FtsL family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10007.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008650296.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3135"/>
    <dbReference type="EnsemblBacteria" id="ABF10007">
        <property type="protein sequence ID" value="ABF10007"/>
        <property type="gene ID" value="Rmet_3135"/>
    </dbReference>
    <dbReference type="GeneID" id="60820489"/>
    <dbReference type="KEGG" id="rme:Rmet_3135"/>
    <dbReference type="eggNOG" id="COG3116">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_156524_0_1_4"/>
    <dbReference type="OMA" id="DWSRLQY"/>
    <dbReference type="OrthoDB" id="1803426at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032153">
        <property type="term" value="C:cell division site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005887">
        <property type="term" value="C:integral component of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043093">
        <property type="term" value="P:FtsZ-dependent cytokinesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00910">
        <property type="entry name" value="FtsL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011922">
        <property type="entry name" value="Cell_div_FtsL"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04999">
        <property type="entry name" value="FtsL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02209">
        <property type="entry name" value="ftsL_broad"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="6 8" id="KW-0132">Cell division</keyword>
    <keyword evidence="6" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="2 6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193201" description="Cell division protein FtsL" evidence="7">
        <location>
            <begin position="27"/>
            <end position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00910"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ABF10007.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="105" mass="11532" checksum="0551E98A76B93B1C" modified="2006-05-30" version="1">MNRLTFFLLAALLFCALSLVSAQHQARTLFVALERAQSEEKQLDIDWSRLQYQQSSLSKSARIAEAARTQLKMSPAVAGRTQYLQGVVLPSTPPDAEQPAEGAAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LIT5</accession>
    <name>Q1LIT5_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Carbohydrate kinase, PfkB family</fullName>
            <ecNumber evidence="4">2.7.1.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Rmet_3069</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.7.1.-" evidence="4"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09941.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517569.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3069"/>
    <dbReference type="EnsemblBacteria" id="ABF09941">
        <property type="protein sequence ID" value="ABF09941"/>
        <property type="gene ID" value="Rmet_3069"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3069"/>
    <dbReference type="eggNOG" id="COG0524">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027634_5_2_4"/>
    <dbReference type="OMA" id="SQQIARM"/>
    <dbReference type="OrthoDB" id="1604782at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016773">
        <property type="term" value="F:phosphotransferase activity, alcohol group as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1190.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002173">
        <property type="entry name" value="Carboh/pur_kinase_PfkB_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011611">
        <property type="entry name" value="PfkB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029056">
        <property type="entry name" value="Ribokinase-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00294">
        <property type="entry name" value="PfkB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53613">
        <property type="entry name" value="SSF53613"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00583">
        <property type="entry name" value="PFKB_KINASES_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 4" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PfkB" evidence="3">
        <location>
            <begin position="27"/>
            <end position="294"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF09941.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="315" mass="34493" checksum="9198530FF121B080" modified="2006-05-30" version="1">MSSLICGSVAYDTIMTFDGRFREHILPDQIHMLNVSFLVPGMRREFGGCAGNIAYTLKMLGGDPIVMATVGQDAEPYLQYLRNLEIRTDHIRVLPETFTAQAMITTDLDNNQITAFHPGAMGQSQLNRVQDALATGNRPAVGIVAPDSREGMLHHAQQFAAEGIPFIFDLGQAMPLFNGEDLRQFIELASYVTVNDYEAQVLLSRTAWTSAEVAAKVRAFIVTHGERGASVFADGKQYAIPAVPAERVVDPTGCGDAFRGGLLHGIENGMDWETTGRLASLMGSLKIAQQGPQNHWMSCDEIGSRFQSVFGYRYA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LJ07</accession>
    <name>Q1LJ07_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Transcriptional regulator, LysR family, acts on the phosphonate ptx genes</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">ptxR</name>
        <name evidence="3" type="ordered locus">Rmet_2996</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the LysR transcriptional regulatory family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09869.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2996"/>
    <dbReference type="EnsemblBacteria" id="ABF09869">
        <property type="protein sequence ID" value="ABF09869"/>
        <property type="gene ID" value="Rmet_2996"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2996"/>
    <dbReference type="eggNOG" id="COG0583">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039613_6_1_4"/>
    <dbReference type="OMA" id="EERSIDC"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005119">
        <property type="entry name" value="LysR_subst-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000847">
        <property type="entry name" value="Tscrpt_reg_HTH_LysR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00126">
        <property type="entry name" value="HTH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03466">
        <property type="entry name" value="LysR_substrate"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00039">
        <property type="entry name" value="HTHLYSR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50931">
        <property type="entry name" value="HTH_LYSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH lysR-type" evidence="2">
        <location>
            <begin position="1"/>
            <end position="41"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF09869.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="274" mass="30708" checksum="87511A4FCDCDB039" modified="2006-05-30" version="1">MTAAAKLLDLQQPTISAHIQRLENEYGVELFLRQGRRLELTTFGRTLLDYTRRAFSGEEDAHALLAAAKNRFVGRLVIHAIGPYNVVPVLKAFGSRHPQVEVSVRVGDSRSITEKLLDYQGDVGVVLNHAEHPELHCMPYRSQRLVVFANREHALARCGEIVLKDLQSQRFVIREEGSTTRRVFESELIARNINIQVALEMGSREAVREAVAQGIGLGVVAETAYVPDPRLVKLKILDTAMATHVDFICRRERQNAPLIATIFDLAKEVRRGLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LJ14</accession>
    <name>Q1LJ14_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 12">Epoxyqueuosine reductase QueH</fullName>
            <ecNumber evidence="5 12">1.17.99.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11 12">Queuosine biosynthesis protein QueH</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="primary">queH</name>
        <name evidence="13" type="ordered locus">Rmet_2989</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2 12">Catalyzes the conversion of epoxyqueuosine (oQ) to queuosine (Q), which is a hypermodified base found in the wobble positions of tRNA(Asp), tRNA(Asn), tRNA(His) and tRNA(Tyr).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 12">
            <text>AH2 + epoxyqueuosine(34) in tRNA = A + H2O + queuosine(34) in tRNA</text>
            <dbReference type="Rhea" id="RHEA:32159"/>
            <dbReference type="Rhea" id="RHEA-COMP:10345"/>
            <dbReference type="Rhea" id="RHEA-COMP:10346"/>
            <dbReference type="ChEBI" id="CHEBI:13193"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:17499"/>
            <dbReference type="ChEBI" id="CHEBI:82831"/>
            <dbReference type="ChEBI" id="CHEBI:82834"/>
            <dbReference type="EC" id="1.17.99.6"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3 12">tRNA modification; tRNA-queuosine biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 12">Belongs to the QueH family.</text>
    </comment>
    <dbReference type="EC" id="1.17.99.6" evidence="5 12"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09862.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_003056085.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2989"/>
    <dbReference type="EnsemblBacteria" id="ABF09862">
        <property type="protein sequence ID" value="ABF09862"/>
        <property type="gene ID" value="Rmet_2989"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2989"/>
    <dbReference type="eggNOG" id="COG1636">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_088177_0_0_4"/>
    <dbReference type="OMA" id="PNIHPYT"/>
    <dbReference type="OrthoDB" id="1078220at2"/>
    <dbReference type="UniPathway" id="UPA00392"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052693">
        <property type="term" value="F:epoxyqueuosine reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008616">
        <property type="term" value="P:queuosine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008033">
        <property type="term" value="P:tRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02089">
        <property type="entry name" value="QueH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003828">
        <property type="entry name" value="QueH"/>
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        <location>
            <position position="24"/>
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        <ligand>
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        <location>
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        <ligand>
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            <position position="106"/>
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        <ligand>
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        <location>
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            <end position="188"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00002268"/>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00008207"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00012622"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00016895"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00031446"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="HAMAP-Rule" id="MF_02089"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="ABF09862.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="222" mass="25798" checksum="AC9664E94E3D7846" modified="2006-05-30" version="1">MNTTVERKALALPGGHDKVLLHSCCAPCSGEVMEAMLASGIDYTIFFYNPNIHPLKEYELRKNENIRFAEQFGVPFVDADYDRDNWFERAKGMEHEPERGVRCTMCFDMRFERTALYAHEHGFPVITSSLGISRWKNMQQINDCGVRAAAKYSGLMYWEYNWRKGGGSARMIEISKRENFYQQEYCGCVYSLRDTNRHRVESGRERIQLGVTFYGDEQPPKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="51" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LJH2</accession>
    <name>Q1LJH2_CUPMC</name>
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        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name evidence="1" type="ordered locus">Rmet_2831</name>
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        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
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    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
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    <dbReference type="OMA" id="ANYRINR"/>
    <dbReference type="Proteomes" id="UP000002429">
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    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
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        <source>
            <dbReference type="EMBL" id="ABF09704.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="221" mass="24801" checksum="E2EFA803CDED3025" modified="2006-05-30" version="1">MPAKPEWRDSLEARKRPIRVLALLEALADASIFEAPLRVVHELAYLSNVLAPVFELAPFSASLLKRQGGPYYPELQDTIDLLVGRGMVFASGIRYEPVPEEKRYRLHANYRINRALAGSALAAYHEVYADTGEPFFIGELCAAYSMLADQELGQAFRFDARYADKDVDNNEVIDFGQWVSAASTNFSRNAAMSFRPGESLQPAERIFMYIEHVQRKVAHGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="57" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LJH6</accession>
    <name>Q1LJH6_CUPMC</name>
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        <submittedName>
            <fullName evidence="4">TraY/DotA-like type IV secretion system protein</fullName>
        </submittedName>
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    <gene>
        <name evidence="4" type="primary">traY</name>
        <name evidence="4" type="ordered locus">Rmet_2827</name>
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    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
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        <lineage>
            <taxon>Bacteria</taxon>
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        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
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        </source>
    </reference>
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    <dbReference type="EnsemblBacteria" id="ABF09700">
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    <dbReference type="OrthoDB" id="1740269at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="entry name" value="DotA_TraY"/>
    </dbReference>
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    </dbReference>
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    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
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        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193608" evidence="3">
        <location>
            <begin position="22"/>
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        <location>
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        <location>
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        <location>
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        <location>
            <begin position="648"/>
            <end position="673"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="715"/>
            <end position="751"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF09700.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="751" mass="80083" checksum="D68EC975202F3DBB" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="110" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LJQ9</accession>
    <name>Q1LJQ9_CUPMC</name>
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        <recommendedName>
            <fullName evidence="3">Pseudouridine synthase</fullName>
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    </protein>
    <gene>
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        <name evidence="6" type="ordered locus">Rmet_2744</name>
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        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
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    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the pseudouridine synthase RsuA family.</text>
    </comment>
    <dbReference type="EC" id="5.4.99.-" evidence="3"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09617.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="STRING" id="266264.Rmet_2744"/>
    <dbReference type="EnsemblBacteria" id="ABF09617">
        <property type="protein sequence ID" value="ABF09617"/>
        <property type="gene ID" value="Rmet_2744"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2744"/>
    <dbReference type="eggNOG" id="COG1187">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024979_1_2_4"/>
    <dbReference type="OMA" id="HVPKTYE"/>
    <dbReference type="OrthoDB" id="1037615at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
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        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120159">
        <property type="term" value="F:rRNA pseudouridine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000455">
        <property type="term" value="P:enzyme-directed rRNA pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.290.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1560">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.580">
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PsdUridine_s_RsuA/RluB/E/F_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020103">
        <property type="entry name" value="PsdUridine_synth_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006145">
        <property type="entry name" value="PsdUridine_synth_RsuA/RluA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000748">
        <property type="entry name" value="PsdUridine_synth_RsuA/RluB/E/F"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018496">
        <property type="entry name" value="PsdUridine_synth_RsuA/RluB_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002942">
        <property type="entry name" value="S4_RNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036986">
        <property type="entry name" value="S4_RNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020094">
        <property type="entry name" value="TruA/RsuA/RluB/E/F_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00849">
        <property type="entry name" value="PseudoU_synth_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01479">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55120">
        <property type="entry name" value="SSF55120"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00093">
        <property type="entry name" value="TIGR00093"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01149">
        <property type="entry name" value="PSI_RSU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0413">Isomerase</keyword>
    <keyword evidence="6" id="KW-0456">Lyase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="S4" evidence="5">
        <location>
            <begin position="5"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="domain" description="PseudoU_synth_2" evidence="4">
        <location>
            <begin position="64"/>
            <end position="195"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008348"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABF09617.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="238" mass="26158" checksum="D555288F9EC2FCE3" modified="2006-05-30" version="1">MATTLDRILQSQGFGTRRYCGDLVAAGLVEVNGELCEDPGARFEVDGLRLMVDGEEWLACTKAYVMLNKPTGYECSQRPRHHPSVYNLLPVPLRQREVQAVGRLDHDTTGLLLLTDDGQFIHAQTSPKKQVPKIYEVTTFDPVTPEQVEALRTGVKLDDEPAPIAADACEATGERSLRLTLLQGKYHQVKRMVAAVGNHVTALHRSAIGGLVLDPALGPGEWRWLTPEDLAALRDGAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-10-07" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LK69</accession>
    <name>Q1LK69_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative DNA-binding transcriptional regulator (LysR-type)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_2580</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the LysR transcriptional regulatory family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09457.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2580"/>
    <dbReference type="EnsemblBacteria" id="ABF09457">
        <property type="protein sequence ID" value="ABF09457"/>
        <property type="gene ID" value="Rmet_2580"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2580"/>
    <dbReference type="eggNOG" id="COG0583">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039613_35_1_4"/>
    <dbReference type="OMA" id="QIRGLGC"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005119">
        <property type="entry name" value="LysR_subst-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000847">
        <property type="entry name" value="Tscrpt_reg_HTH_LysR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00126">
        <property type="entry name" value="HTH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03466">
        <property type="entry name" value="LysR_substrate"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50931">
        <property type="entry name" value="HTH_LYSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH lysR-type" evidence="2">
        <location>
            <begin position="34"/>
            <end position="91"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF09457.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="342" mass="37532" checksum="A15F769668BE0981" modified="2006-05-30" version="1">MGRGVSMLGSLGQREYIGGHSIRSSRSENLNMPLSLESLEVLDAIERKGSFAAAAHEMGKVPSALTYVVRKLEEDLDVLLFDRRRHRAELTPAGRALLDEGRHLLRAADDLERRIKRLATGWEASLNITVDDLVNFRALLPVIQDFYAENTATRLRFSKEVLGGAWDALVSNRADLIIGGAYEAPSTQGFQVRALGAMPFVFAVAAHHPLAAVEGPLTTIEIAKHRIVAVGDTSRNLPARTHGVLAGQDVLVVPTMRDKLEAQIRGLGCGWLPAPLAQPHIESGVLQARETVEVRAPGNFKVAWRTNTRGKALQWWTNKLEDPRLAQALLLQPPHSPLDQTD</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LK71</accession>
    <name>Q1LK71_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Cobalamin biosynthesis protein CobD</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">cobD</name>
        <name evidence="11" type="ordered locus">Rmet_2578</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Converts cobyric acid to cobinamide by the addition of aminopropanol on the F carboxylic group.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3 10">Cofactor biosynthesis; adenosylcobalamin biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 10">Cell membrane</location>
            <topology evidence="2 10">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 10">Belongs to the CobD/CbiB family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09455.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517158.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2578"/>
    <dbReference type="TCDB" id="9.B.125.1.2">
        <property type="family name" value="the ampe/cobd (ampe/cobd) family"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF09455">
        <property type="protein sequence ID" value="ABF09455"/>
        <property type="gene ID" value="Rmet_2578"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2578"/>
    <dbReference type="eggNOG" id="COG1270">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_054212_1_0_4"/>
    <dbReference type="OMA" id="YAWRNHA"/>
    <dbReference type="OrthoDB" id="2029688at2"/>
    <dbReference type="UniPathway" id="UPA00148"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015420">
        <property type="term" value="F:ABC-type vitamin B12 transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048472">
        <property type="term" value="F:threonine-phosphate decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009236">
        <property type="term" value="P:cobalamin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00024">
        <property type="entry name" value="CobD_CbiB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031347">
        <property type="entry name" value="AmpE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004485">
        <property type="entry name" value="Cobalamin_biosynth_CobD/CbiB"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17113">
        <property type="entry name" value="AmpE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03186">
        <property type="entry name" value="CobD_Cbib"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 10" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 10" id="KW-0169">Cobalamin biosynthesis</keyword>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 10 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="48"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="74"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="148"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="304"/>
            <end position="325"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022573"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ABF09455.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="326" mass="35914" checksum="E9D44B6CA06F815C" modified="2006-05-30" version="1">MTFVSILLALIAEQFRALGRNNPIFDIVRVLGDRAEHAFDTGRPRDAALAWLTVVLPLTLAVVIVHYLLSSISIALTLVWNVLLLYLTLGFRQFSHYFTDIHEALNRDDLHTARTILHEWTGLDTVEMPVSEIVRHTLEAAIVAVHRHVFGVFFWFLVPIGPGGVILYRTAEYLSRQWNSPSNERSPALGCFAAQAFYVLDWVPSRLTSIGFAIVGNFEDAVYAWRNHARKWNDAVNGILLASGGGALGVRLGAPLAEDDSSVILRMSVAGVDYAPGMDDGSDPVPPEFGSDPGVRTLQSAVGLVWRAVVLWMILLAMISLALWVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LK87</accession>
    <name>Q1LK87_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Phosphoprotein phosphatase</fullName>
            <ecNumber evidence="2">3.1.3.16</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_2562</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EC" id="3.1.3.16" evidence="2"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09439.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517142.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2562"/>
    <dbReference type="EnsemblBacteria" id="ABF09439">
        <property type="protein sequence ID" value="ABF09439"/>
        <property type="gene ID" value="Rmet_2562"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2562"/>
    <dbReference type="eggNOG" id="COG0631">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_034545_0_3_4"/>
    <dbReference type="OMA" id="EDHTWVA"/>
    <dbReference type="OrthoDB" id="1835483at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017018">
        <property type="term" value="F:myosin phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006470">
        <property type="term" value="P:protein dephosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00143">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015655">
        <property type="entry name" value="PP2C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036457">
        <property type="entry name" value="PPM-type_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001932">
        <property type="entry name" value="PPM-type_phosphatase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13832">
        <property type="entry name" value="PTHR13832"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00331">
        <property type="entry name" value="PP2C_SIG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00332">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81606">
        <property type="entry name" value="SSF81606"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51746">
        <property type="entry name" value="PPM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PPM-type phosphatase" evidence="1">
        <location>
            <begin position="10"/>
            <end position="239"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF09439.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="256" mass="27477" checksum="7BBB81473E902884" modified="2006-05-30" version="1">MENTTAFQWESAAHTDVGLARARNEDAWLAAPGLWVVADGMGGHAHGDFASRSIVEALSTLSLPGDLDQAIADTRAAILGVNQLLMDEARHARVRVIGSTVVALVSRGHQCACLWAGDSRLYLLRDNHLQQLTRDHSHVEQLRARGLITADEAKHHPAHNAITRAVGAAATLDLETLTLDVRDGDMFLLCSDGLYNDVDDTDIATVLASWDCRHAADELVRLALARGGQDNVTVVVIRASEADGSSESYLSPDTTG</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="95" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LKB6</accession>
    <name>Q1LKB6_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">3-Oxoacyl-(Acyl-carrier-protein) reductase</fullName>
            <ecNumber evidence="2">1.1.1.100</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_2533</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.100" evidence="2"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09410.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517109.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2533"/>
    <dbReference type="EnsemblBacteria" id="ABF09410">
        <property type="protein sequence ID" value="ABF09410"/>
        <property type="gene ID" value="Rmet_2533"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2533"/>
    <dbReference type="eggNOG" id="COG1028">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010194_1_3_4"/>
    <dbReference type="OMA" id="IASHITY"/>
    <dbReference type="OrthoDB" id="1601931at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004316">
        <property type="term" value="F:3-oxoacyl-[acyl-carrier-protein] reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00061">
        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU000363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF09410.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="276" mass="29351" checksum="0FE07F6AC62B586C" modified="2006-05-30" version="1">MSRKLEGKVAIVTGAGRGIGRCIALKFASEGAGVVVNDLDAAPANDVVEEIRRSGGKALAFACNVTEPDFGERLVKATLEAFGGIDIIVNNAGYTWDNVIQKMSDEQWDAVIDCHLKAPFNLLRAAQPYFREVSKAEAAEGREVFRKVVNISSISGTQGNAGQVNYSAAKAGVMGMTKTLAKEWGRMKVNVNCVAFGFISTRLTEATTEEKTVSIEGRDIKVGVNPERIAQASKEMALGRPGTAEEAAGSVYMFCIPESNYVTGQTIICGGGRGGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LKE8</accession>
    <name>Q1LKE8_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Glutathione s-transferase transmembrane protein</fullName>
            <ecNumber evidence="4">2.5.1.18</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">gstF</name>
        <name evidence="4" type="ordered locus">Rmet_2501</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the AHA1 family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.18" evidence="4"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09378.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517079.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2501"/>
    <dbReference type="EnsemblBacteria" id="ABF09378">
        <property type="protein sequence ID" value="ABF09378"/>
        <property type="gene ID" value="Rmet_2501"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2501"/>
    <dbReference type="eggNOG" id="COG0625">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG3832">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011226_5_2_4"/>
    <dbReference type="OMA" id="WISLINC"/>
    <dbReference type="OrthoDB" id="1383885at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004364">
        <property type="term" value="F:glutathione transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006749">
        <property type="term" value="P:glutathione metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.530.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013538">
        <property type="entry name" value="Activator_of_Hsp90_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010987">
        <property type="entry name" value="Glutathione-S-Trfase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036282">
        <property type="entry name" value="Glutathione-S-Trfase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040079">
        <property type="entry name" value="Glutathione_S-Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004045">
        <property type="entry name" value="Glutathione_S-Trfase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004046">
        <property type="entry name" value="GST_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023393">
        <property type="entry name" value="START-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08327">
        <property type="entry name" value="AHSA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00043">
        <property type="entry name" value="GST_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13417">
        <property type="entry name" value="GST_N_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00019">
        <property type="entry name" value="Glutathione_Transferase_(cytos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47616">
        <property type="entry name" value="SSF47616"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="SSF52833"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50405">
        <property type="entry name" value="GST_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50404">
        <property type="entry name" value="GST_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <feature type="domain" description="GST N-terminal" evidence="2">
        <location>
            <begin position="153"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="domain" description="GST C-terminal" evidence="3">
        <location>
            <begin position="235"/>
            <end position="360"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006817"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF09378.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="360" mass="40090" checksum="7E2B8870BAB76FF7" modified="2006-05-30" version="1">MTEAATFHLEMTRQIRAPRERVFDAFTDQAALAAWHCPRGMRVLEASADARVGGRYRLVMGSRDGSQHMVGGEYQKIDRANFLAYTWQWEGGELPEGTRTLIEVTLTDKDGGTLLHMRHSGFPDTATRDAHTSGWQSVFNRLSDYVDAEGSAGTVTVYGDGRSTYVRTVRMALAEKGIAYKLDPLTPRDPELLKHNPFGRIPAFTDGPLEFFETRAILSYIEEAFDGPSLISQAGPTARARCEQWISLINCHAYDAMVRRYVLQYVFPSGENGQPDRKTIDAALPDIAKQLDALEHAYGGRDFLAGNTLTMADLFFAPIVEYLGRFPESAAMLESRPNIRRAHAAMRARPSYAATQPNFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LKH0</accession>
    <name>Q1LKH0_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Leucine/isoleucine/valine transporter subunit, membrane component of ABC superfamily</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">livH1</name>
        <name evidence="7" type="ordered locus">Rmet_2479</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09356.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517066.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2479"/>
    <dbReference type="EnsemblBacteria" id="ABF09356">
        <property type="protein sequence ID" value="ABF09356"/>
        <property type="gene ID" value="Rmet_2479"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2479"/>
    <dbReference type="eggNOG" id="COG0559">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039929_3_1_4"/>
    <dbReference type="OMA" id="NMGWFLI"/>
    <dbReference type="OrthoDB" id="1529037at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001851">
        <property type="entry name" value="ABC_transp_permease"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02653">
        <property type="entry name" value="BPD_transp_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="20"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="46"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="71"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="102"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="155"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="204"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="282"/>
            <end position="301"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABF09356.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="308" mass="32271" checksum="5D10158DF92FA7B6" modified="2006-05-30" version="1">MNEFLPQFTQQLVNGLTLGAIYALIAIGYTMVYGIIGMINFAHGEIYMIGAYVGLVTLTAIGAQAGYPLPLVLGAALIVSVAVTGCYGYAVERVAYRPLRGGPRLVPLISAIGMSIFLQNYVQIGQGARDVSVPILISGAIEFQMGGDFTVTVPYSRLLIVGVTLVLMIVLTLFIGHSRMGRACRACAEDMRMANLLGIDTNRVISFTFVLGAMLAAVGGVLIGLTIGKLNPFIGFIAGIKAFTAAVLGGIGSIPGAMLGGVLLGLAETFASGYMPAEYKDVVAFGLLVLVLLFRPTGLLGKPDVEKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="65" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LKK2</accession>
    <name>Q1LKK2_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Na+/H+ antiporter NhaD or related arsenite permease transmembrane protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_2447</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09324.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517040.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2447"/>
    <dbReference type="EnsemblBacteria" id="ABF09324">
        <property type="protein sequence ID" value="ABF09324"/>
        <property type="gene ID" value="Rmet_2447"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2447"/>
    <dbReference type="eggNOG" id="COG1055">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_034923_0_0_4"/>
    <dbReference type="OMA" id="WGVPFAG"/>
    <dbReference type="OrthoDB" id="626718at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031566">
        <property type="entry name" value="CitMHS_2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16980">
        <property type="entry name" value="CitMHS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1 3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193529" evidence="2">
        <location>
            <begin position="26"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="35"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="67"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="99"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="130"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="165"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="209"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="258"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="297"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="335"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="374"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="410"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="451"/>
            <end position="474"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF09324.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="476" mass="51408" checksum="ACD03D1BED52C737" modified="2006-05-30" version="1">MRRVSTLLPHCLLALVGLLPGWGHAADLDGAALAPIWGLPFAGILLSIALFPLFAPKLWHYHYGKIAAAWGVLFLVPFATVFGMHTAAANVVHALLSEYIPFIVLLTALYVVAGGICVRGNLHGTPKLNTGILALGTLLASIMGTTGAAMLLIRPLLRANDNRRHVAHVVVFFIFLVANAGGALTPLGDPPLFLGFLKGVDFFWTMRNILPETIFMWVLLLALFYVIDRHYYLNREEELPVRQDPTPDSRGIRIDGKVNFVLLLVVIGLVLMSGLWKPGIVFDVMGTDVPLPAVLRDVLLVVVTVASLIVTPHVARAGNEFNWEPILEVGKLFAGIFLTIIPVIAMLKAGTDGAFAGVIRAVSDGNGQPIDSMYFWATGILSSFLDNAPTYLVFFNTAGGDPATLMTRDASTLAAISAGAVFMGANTYIGNAPNLMVKAIAESRGVRMPSFFGYMAWSCTVLLPLFLVMTLLFFHV</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="59" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LKX7</accession>
    <name>Q1LKX7_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_2322</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09199.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_003116823.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2322"/>
    <dbReference type="EnsemblBacteria" id="ABF09199">
        <property type="protein sequence ID" value="ABF09199"/>
        <property type="gene ID" value="Rmet_2322"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2322"/>
    <dbReference type="eggNOG" id="ENOG502Z8W2">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_106522_1_0_4"/>
    <dbReference type="OMA" id="TWYPAES"/>
    <dbReference type="OrthoDB" id="1140071at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021693">
        <property type="entry name" value="DUF3275"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11679">
        <property type="entry name" value="DUF3275"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="110"/>
            <end position="169"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF09199.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="229" mass="24518" checksum="8F668916004D8C78" modified="2006-05-30" version="1">MATPSASEKSVAPIVVPGQLTLRTIRGKNGPFTVGRLATHLGTFEVKDPELEQYPEGKYDGEFIIRYIFPKSYPVGGGMRFEIRASLDGMTLNGIDKLSRDEARSFATQDVDPLDEEQGAQPAATPAKPTKASRPAKPAPVQASADPLVDTTPFGVDAPPPATAAAPGSTEDGDAALFGLLWPLGESVKLDSTIDRRTLRAQIARLGELGYALDFKTQEWSRQAEPKPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="54" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LKY1</accession>
    <name>Q1LKY1_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_2318</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09195.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2318"/>
    <dbReference type="EnsemblBacteria" id="ABF09195">
        <property type="protein sequence ID" value="ABF09195"/>
        <property type="gene ID" value="Rmet_2318"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2318"/>
    <dbReference type="eggNOG" id="ENOG5031C7I">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_165361_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021436">
        <property type="entry name" value="DUF3085"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11284">
        <property type="entry name" value="DUF3085"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF09195.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="86" mass="9551" checksum="80DA14469C865B1F" modified="2006-05-30" version="1">MYFLAERGERRPDGRQALLAYAVGCNPDTDPFDDWWHLAGRELGGDDFAEYFDPKDGLFTRLQHSADDLVLSATATHLSLAVVPPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="118" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LKZ4</accession>
    <name>Q1LKZ4_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Lead/cadmium-transporting ATPase (Cation efflux ATPase)</fullName>
            <ecNumber evidence="9">3.6.3.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">cadA</name>
        <name evidence="9" type="ordered locus">Rmet_2303</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cell membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IB subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.3.-" evidence="9"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09182.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_005408895.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2303"/>
    <dbReference type="EnsemblBacteria" id="ABF09182">
        <property type="protein sequence ID" value="ABF09182"/>
        <property type="gene ID" value="Rmet_2303"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2303"/>
    <dbReference type="eggNOG" id="COG2217">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001771_6_1_4"/>
    <dbReference type="OMA" id="HLHLMET"/>
    <dbReference type="OrthoDB" id="237367at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019829">
        <property type="term" value="F:ATPase-coupled cation transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00371">
        <property type="entry name" value="HMA"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1110.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023299">
        <property type="entry name" value="ATPase_P-typ_cyto_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018303">
        <property type="entry name" value="ATPase_P-typ_P_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023298">
        <property type="entry name" value="ATPase_P-typ_TM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008250">
        <property type="entry name" value="ATPase_P-typ_transduc_dom_A_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006121">
        <property type="entry name" value="HMA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036163">
        <property type="entry name" value="HMA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027256">
        <property type="entry name" value="P-typ_ATPase_IB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001757">
        <property type="entry name" value="P_typ_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044492">
        <property type="entry name" value="P_typ_ATPase_HD_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00403">
        <property type="entry name" value="HMA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00941">
        <property type="entry name" value="CDATPASE"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00027">
        <property type="entry name" value="p-type_atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55008">
        <property type="entry name" value="SSF55008"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="SSF56784"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81653">
        <property type="entry name" value="SSF81653"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81665">
        <property type="entry name" value="SSF81665"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01525">
        <property type="entry name" value="ATPase-IB_hvy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01494">
        <property type="entry name" value="ATPase_P-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00154">
        <property type="entry name" value="ATPASE_E1_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50846">
        <property type="entry name" value="HMA_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-1278">Translocase</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="367"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="393"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="596"/>
            <end position="621"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="627"/>
            <end position="654"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="929"/>
            <end position="951"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="957"/>
            <end position="979"/>
        </location>
    </feature>
    <feature type="domain" description="HMA" evidence="8">
        <location>
            <begin position="44"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="domain" description="HMA" evidence="8">
        <location>
            <begin position="115"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="domain" description="HMA" evidence="8">
        <location>
            <begin position="215"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="domain" description="HMA" evidence="8">
        <location>
            <begin position="286"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="14"/>
            <end position="32"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU362081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ABF09182.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="984" mass="104651" checksum="8E605371822E8FC8" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LL79</accession>
    <name>Q1LL79_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Alkanesulfonate monooxygenase, FMNH(2)-dependent</fullName>
            <ecNumber evidence="2">1.14.14.5</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">ssuD1</name>
        <name evidence="2" type="ordered locus">Rmet_2218</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EC" id="1.14.14.5" evidence="2"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09097.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516925.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2218"/>
    <dbReference type="EnsemblBacteria" id="ABF09097">
        <property type="protein sequence ID" value="ABF09097"/>
        <property type="gene ID" value="Rmet_2218"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2218"/>
    <dbReference type="eggNOG" id="COG2141">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027853_1_4_4"/>
    <dbReference type="OMA" id="PRIQVAF"/>
    <dbReference type="OrthoDB" id="919913at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008726">
        <property type="term" value="F:alkanesulfonate monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011251">
        <property type="entry name" value="Luciferase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036661">
        <property type="entry name" value="Luciferase-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00296">
        <property type="entry name" value="Bac_luciferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51679">
        <property type="entry name" value="SSF51679"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Bac_luciferase" evidence="1">
        <location>
            <begin position="15"/>
            <end position="323"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF09097.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="363" mass="40432" checksum="EF6CAE787862EA77" modified="2006-05-30" version="1">MSVDIIGMIQSQKQSEIHPPDGPVIDRDYVRAFAQAHEQAGFDRILVPHHSTGPSATLTISYAATVTERIHFMLAHRPGFTAPTLAARQIATLDQFSGGRLGVHFISGGSDDEQRRDGDYLDHDQRYARTDEYLGILRRIWTEHQPFDHEGTFYRFQRGFSEVKPAQQPHVPIYFGGASEAALAVAGKHADVYALWGESLDQVRELTTRVRAEAARHAREVRFSVSFRPVLADTEEKAWARADSILERTRALRVQAGYSRGGPQQSEGARRLLAAADKGDRVDQRLWTAIARETGGRSNSTGLVGTPEQVAEALLAYYDLGVSTFLLRGFDPLDDAVDYGRELIPRVRALVAQRDAERQRKAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="60" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LLD2</accession>
    <name>Q1LLD2_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_2165</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09044.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516874.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2165"/>
    <dbReference type="EnsemblBacteria" id="ABF09044">
        <property type="protein sequence ID" value="ABF09044"/>
        <property type="gene ID" value="Rmet_2165"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2165"/>
    <dbReference type="eggNOG" id="ENOG502ZA36">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1007492_0_0_4"/>
    <dbReference type="OMA" id="TFEVFEG"/>
    <dbReference type="OrthoDB" id="1306712at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF09044.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="290" mass="30241" checksum="D9B2D634B22AAF31" modified="2006-05-30" version="1">MGTSTSSAGAPGGSPFDPPWLTDVEQGIDTSQSDAPLAPVPPAPQPDGGADDTETGADADAVQTVGPNIAPPGRYGEARRALSSFVKGGGSADLRKGINRLVNKGMGGSKRAASRMQSTVTAAAALGGLLSAARAGGDPGIDAWVASVKQRGLSATDIALEVAERLLPNGGSIDEESAKHAMDQAIIKLYDTDPNADIFNLTDDQIADVMTYTIAYDVYNRVQLELGRVFEKLKYSAKVVHERLGQVLDYVTGVVADAMKGAREGKKPRSMREVSAKAMDDAMFVFGTAE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-01-15" modified="2022-08-03" version="95" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LLF7</accession>
    <name>SYE_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Glutamate--tRNA ligase</fullName>
            <ecNumber evidence="1">6.1.1.17</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Glutamyl-tRNA synthetase</fullName>
            <shortName evidence="1">GluRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">gltX</name>
        <name type="ordered locus">Rmet_2140</name>
    </gene>
    <organism>
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu)</text>
            <dbReference type="Rhea" id="RHEA:23540"/>
            <dbReference type="Rhea" id="RHEA-COMP:9663"/>
            <dbReference type="Rhea" id="RHEA-COMP:9680"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78520"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.17"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-I aminoacyl-tRNA synthetase family. Glutamate--tRNA ligase type 1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.17" evidence="1"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09019.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516848.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
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    <dbReference type="AlphaFoldDB" id="Q1LLF7"/>
    <dbReference type="SMR" id="Q1LLF7"/>
    <dbReference type="STRING" id="266264.Rmet_2140"/>
    <dbReference type="EnsemblBacteria" id="ABF09019">
        <property type="protein sequence ID" value="ABF09019"/>
        <property type="gene ID" value="Rmet_2140"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2140"/>
    <dbReference type="eggNOG" id="COG0008">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015768_6_0_4"/>
    <dbReference type="OMA" id="WDEGPFF"/>
    <dbReference type="OrthoDB" id="1409413at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004818">
        <property type="term" value="F:glutamate-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006424">
        <property type="term" value="P:glutamyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00808">
        <property type="entry name" value="GluRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.350">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00022">
        <property type="entry name" value="Glu_tRNA_synth_type1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045462">
        <property type="entry name" value="aa-tRNA-synth_I_cd-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020751">
        <property type="entry name" value="aa-tRNA-synth_I_codon-bd_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001412">
        <property type="entry name" value="aa-tRNA-synth_I_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008925">
        <property type="entry name" value="aa_tRNA-synth_I_cd-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004527">
        <property type="entry name" value="Glu-tRNA-ligase_bac/mito"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000924">
        <property type="entry name" value="Glu/Gln-tRNA-synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020058">
        <property type="entry name" value="Glu/Gln-tRNA-synth_Ib_cat-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033910">
        <property type="entry name" value="GluRS_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19269">
        <property type="entry name" value="Anticodon_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00749">
        <property type="entry name" value="tRNA-synt_1c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00987">
        <property type="entry name" value="TRNASYNTHGLU"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48163">
        <property type="entry name" value="SSF48163"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00464">
        <property type="entry name" value="gltX_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00178">
        <property type="entry name" value="AA_TRNA_LIGASE_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_1000001946" description="Glutamate--tRNA ligase">
        <location>
            <begin position="1"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'HIGH' region" evidence="1">
        <location>
            <begin position="11"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'KMSKS' region" evidence="1">
        <location>
            <begin position="243"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="246"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00022"/>
        </source>
    </evidence>
    <sequence length="465" mass="52295" checksum="DC16FD3A2048AAFD" modified="2006-05-30" version="1">MTQRVRTRFAPSPTGFIHLGNIRSAFYPWAFARRMKGDFILRIEDTDVERSTDVAVDVILESMAWLDLDIDEGPFYQMQRMDRYREVVQQMLDNELAYHCYMSTEELDALREAQRAAGEKPRYNGFWRPEPGKVLPEPPAGVQPVVRFKNPIGGSVVWDDAVKGRIEISNDELDDLVIARPDGTPTYNFCVVVDDLDMKITHVIRGDDHVNNTPRQINIIRALGGEVPVYAHLPTVLNEQGEKMSKRHGALPVTGYRDEGYLPEAVLNYLARLGWAHGDAEIFSREQFVEWFDLEHLGKSPAQYNPEKLAWLNNHYIKVGDNTRLADLTRPFIEALGGKVEGANLVDVIALVKDRANTLKEVAQTAMLFYRGEPQADAALKAEHLTDEIRPALQALATQLAALPEWKREAISAAFKAVLAEFGLKMPKLAMPVRLLVAGQLQTPSIDAVLELFGRDTVLRRLAAA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-05-20" modified="2022-08-03" version="105" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LLR6</accession>
    <name>IF2_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Translation initiation factor IF-2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">infB</name>
        <name type="ordered locus">Rmet_2031</name>
    </gene>
    <organism>
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">One of the essential components for the initiation of protein synthesis. Protects formylmethionyl-tRNA from spontaneous hydrolysis and promotes its binding to the 30S ribosomal subunits. Also involved in the hydrolysis of GTP during the formation of the 70S ribosomal complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. IF-2 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08910.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516746.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q1LLR6"/>
    <dbReference type="SMR" id="Q1LLR6"/>
    <dbReference type="STRING" id="266264.Rmet_2031"/>
    <dbReference type="PRIDE" id="Q1LLR6"/>
    <dbReference type="EnsemblBacteria" id="ABF08910">
        <property type="protein sequence ID" value="ABF08910"/>
        <property type="gene ID" value="Rmet_2031"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2031"/>
    <dbReference type="eggNOG" id="COG0532">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006301_6_0_4"/>
    <dbReference type="OMA" id="RDVMMAG"/>
    <dbReference type="OrthoDB" id="347113at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd03702">
        <property type="entry name" value="IF2_mtIF2_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10050">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00100_B">
        <property type="entry name" value="IF_2_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009061">
        <property type="entry name" value="DNA-bd_dom_put_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013575">
        <property type="entry name" value="IF2_assoc_dom_bac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044145">
        <property type="entry name" value="IF2_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006847">
        <property type="entry name" value="IF2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000795">
        <property type="entry name" value="T_Tr_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000178">
        <property type="entry name" value="TF_IF2_bacterial-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015760">
        <property type="entry name" value="TIF_IF2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023115">
        <property type="entry name" value="TIF_IF2_dom3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036925">
        <property type="entry name" value="TIF_IF2_dom3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43381">
        <property type="entry name" value="PTHR43381"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00009">
        <property type="entry name" value="GTP_EFTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11987">
        <property type="entry name" value="IF-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08364">
        <property type="entry name" value="IF2_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04760">
        <property type="entry name" value="IF2_N"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46955">
        <property type="entry name" value="SSF46955"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="SSF50447"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52156">
        <property type="entry name" value="SSF52156"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00487">
        <property type="entry name" value="IF-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51722">
        <property type="entry name" value="G_TR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01176">
        <property type="entry name" value="IF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0342">GTP-binding</keyword>
    <keyword id="KW-0396">Initiation factor</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000335501" description="Translation initiation factor IF-2">
        <location>
            <begin position="1"/>
            <end position="979"/>
        </location>
    </feature>
    <feature type="domain" description="tr-type G">
        <location>
            <begin position="479"/>
            <end position="646"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="50"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="region of interest" description="G1" evidence="1">
        <location>
            <begin position="488"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="region of interest" description="G2" evidence="1">
        <location>
            <begin position="513"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="region of interest" description="G3" evidence="1">
        <location>
            <begin position="534"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="region of interest" description="G4" evidence="1">
        <location>
            <begin position="588"/>
            <end position="591"/>
        </location>
    </feature>
    <feature type="region of interest" description="G5" evidence="1">
        <location>
            <begin position="624"/>
            <end position="626"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="50"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="86"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="145"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="194"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="279"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="488"/>
            <end position="495"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="534"/>
            <end position="538"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="588"/>
            <end position="591"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00100"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="979" mass="104941" checksum="B72464CECD3E8F1E" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LMF4</accession>
    <name>Q1LMF4_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">ABC transporter, permease protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Rmet_1793</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08672.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516522.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1793"/>
    <dbReference type="EnsemblBacteria" id="ABF08672">
        <property type="protein sequence ID" value="ABF08672"/>
        <property type="gene ID" value="Rmet_1793"/>
    </dbReference>
    <dbReference type="GeneID" id="60825713"/>
    <dbReference type="KEGG" id="rme:Rmet_1793"/>
    <dbReference type="eggNOG" id="COG0577">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_8_10_4"/>
    <dbReference type="OMA" id="NWFGGVY"/>
    <dbReference type="OrthoDB" id="768211at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025857">
        <property type="entry name" value="MacB_PCD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12704">
        <property type="entry name" value="MacB_PCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="260"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="300"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="349"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="domain" description="MacB_PCD" evidence="7">
        <location>
            <begin position="18"/>
            <end position="226"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF12704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ABF08672.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="384" mass="41828" checksum="D509EFFE863EEE60" modified="2006-05-30" version="1">MYLAKLITRNAMRHKLRTALTVLGLVIAVLAYGLLQTVVDAWYAGASAASNARLVTRNAISLTFTLPLSYENRIKGVDGVTMVARSNWFGGVYRDPKNFFAQFAVSDNYLDLYPEFIVPPTQRADYERDRRGALIGRQLADQYGFKIGDVIPIKGTIYPGTWDFVVRGIMEGRDESTITRQMVFHWDYLNETVRKKTARQADQVGVYVIGVDNPDNAAAISRAVDNVFKNSLAETLTETEQAFQLGFVAMSNQIIAAIRVVSYVVIVIVMAVMANAMAMSARERITEYATLKALGFGPGFLSMLVFGESLIISAFGGGLGMLLTPPAARFFKQAVGGVFPVFTVSHDTMAMQAACALVVGLAAAIVPAVQAARVRIVEGLRAIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LMH2</accession>
    <name>Q1LMH2_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Putative FAD/FMN-containing dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Rmet_1775</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the oxygen-dependent FAD-linked oxidoreductase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08654.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516507.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1775"/>
    <dbReference type="EnsemblBacteria" id="ABF08654">
        <property type="protein sequence ID" value="ABF08654"/>
        <property type="gene ID" value="Rmet_1775"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1775"/>
    <dbReference type="eggNOG" id="COG0277">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018354_10_2_4"/>
    <dbReference type="OMA" id="IAGMWPD"/>
    <dbReference type="OrthoDB" id="330793at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.43.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.465.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012951">
        <property type="entry name" value="BBE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016166">
        <property type="entry name" value="FAD-bd_PCMH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036318">
        <property type="entry name" value="FAD-bd_PCMH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016167">
        <property type="entry name" value="FAD-bd_PCMH_sub1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016169">
        <property type="entry name" value="FAD-bd_PCMH_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006094">
        <property type="entry name" value="Oxid_FAD_bind_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006093">
        <property type="entry name" value="Oxy_OxRdtase_FAD_BS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08031">
        <property type="entry name" value="BBE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01565">
        <property type="entry name" value="FAD_binding_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56176">
        <property type="entry name" value="SSF56176"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51387">
        <property type="entry name" value="FAD_PCMH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00862">
        <property type="entry name" value="OX2_COVAL_FAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0274">FAD</keyword>
    <keyword evidence="2" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD-binding PCMH-type" evidence="3">
        <location>
            <begin position="47"/>
            <end position="217"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF08654.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="463" mass="49958" checksum="589E898173E966B5" modified="2006-05-30" version="1">MRSDRQEASGDGGNPGFEAFRQALGGQLVQRGDPDYDMARKVWNGAIDKHPALVVYCADATDVAGAVRFARATGLTIAVRSGGHNLAGLSTCDNGIVIDLSRMKRIDVDVARRRARAEAGLNLGEFDQATLRHGLATTMGVNSDTGIAGLTLGGGFGKLGRKYGLSCDNLEAVEIVTADGERLHASTTEHPDLFWAIRGGGGNFGIVTAFHFRLHPIPARLPVCAVVYPWDQAREAMLHYDAFARAAPDDVAADAALVTQPSGERCLSISACHVGPDGTEETRQAALRPLVEFGNPVDAQLDFVPYLQMQSASDALFARGRRYYWKAQFLRQIRAEAVDALLAAYALAPSPGCLVVFQQVGGAIARVPDEATAYGNRSADFDCFPLAIWDDPADDDKHREWARGLWEAVQPYSTGGVYANNLGDEGAQRTRAAYGVNHSRLVAVKRQYDPDNAFRLNQNIDPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LMW2</accession>
    <name>Q1LMW2_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Transcriptional regulator, LysR-family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_1631</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the LysR transcriptional regulatory family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08514.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516359.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1631"/>
    <dbReference type="EnsemblBacteria" id="ABF08514">
        <property type="protein sequence ID" value="ABF08514"/>
        <property type="gene ID" value="Rmet_1631"/>
    </dbReference>
    <dbReference type="GeneID" id="60822282"/>
    <dbReference type="KEGG" id="rme:Rmet_1631"/>
    <dbReference type="eggNOG" id="COG0583">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039613_6_5_4"/>
    <dbReference type="OMA" id="ELWDIMQ"/>
    <dbReference type="OrthoDB" id="990666at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005119">
        <property type="entry name" value="LysR_subst-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000847">
        <property type="entry name" value="Tscrpt_reg_HTH_LysR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00126">
        <property type="entry name" value="HTH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03466">
        <property type="entry name" value="LysR_substrate"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00039">
        <property type="entry name" value="HTHLYSR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50931">
        <property type="entry name" value="HTH_LYSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH lysR-type" evidence="2">
        <location>
            <begin position="4"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF08514.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="311" mass="32988" checksum="F14AB7F195A0FF86" modified="2006-05-30" version="1">MSALNSDDLACFICVANTQSISRAALELGADQSTVSRQIARLEASLDTRLFHRSGRGVVLTEAGNTLLGYARQVAATLTEARAAVRASTAQGPAQLVIAAQPTIANTAFAAIGTALKTRFPATKLRFVEGLASPILAWLAAGEIDVALLYLPEQQGALKVDILLEEDLTLVTPTSWSHLGPTFQARRLGEVPMILPSTGHGLRVLAESLAARVGTTLNMALECDASNTVSMRLVEDGCGATLLPFAAVADRVAQGRLHSARLVDPVVTRQVALATARNRPPVPELWDIMQTVRLSVRNIVMSGAWPGARLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="98" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LMX8</accession>
    <name>Q1LMX8_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Dipeptide transporter membrane component of ABC superfamily</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">dppB</name>
        <name evidence="7" type="ordered locus">Rmet_1615</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cell membrane</location>
            <topology evidence="5">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the binding-protein-dependent transport system permease family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08498.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1615"/>
    <dbReference type="EnsemblBacteria" id="ABF08498">
        <property type="protein sequence ID" value="ABF08498"/>
        <property type="gene ID" value="Rmet_1615"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1615"/>
    <dbReference type="eggNOG" id="COG0601">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036879_1_0_4"/>
    <dbReference type="OMA" id="AKDYPLM"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="entry name" value="MetI-like"/>
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    </dbReference>
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        <property type="entry name" value="BPD_transp_1_N"/>
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    </dbReference>
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        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <location>
            <begin position="20"/>
            <end position="38"/>
        </location>
    </feature>
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        <location>
            <begin position="109"/>
            <end position="131"/>
        </location>
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        <location>
            <begin position="151"/>
            <end position="177"/>
        </location>
    </feature>
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        <location>
            <begin position="197"/>
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        </location>
    </feature>
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        <location>
            <begin position="255"/>
            <end position="281"/>
        </location>
    </feature>
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        <location>
            <begin position="301"/>
            <end position="320"/>
        </location>
    </feature>
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        <location>
            <begin position="105"/>
            <end position="324"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="ABF08498.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="340" mass="37256" checksum="F625CB05546D57F1" modified="2006-05-30" version="1">MHAEVIVNFLSFFLSRLVKALVVVIGVVVINFFLIRMAPGDPATVMAGEAGAGDATYVTQLREQFGLDQPVLTQLGIYLKGVAKLDLGYSYRNHLPVLDLILDRLPATFLLMSGAFLFSIVMGVLLGVIAARTRYENRRRWIDSAVMSGALLLYATPLFWLSLMAIILFSVVLGWLPAFGMESVGAGYTGLARVQDIALHLVLPTISLGCFFMAVYVRLTRASMLEVMGMDFVKTARAKGVPARRVIRVHVLRNALLPVITFAGIQLGQMAGGAVLTETVFSWPGIGRLMFDALLQRDYQLLLGIFFVTSALVVFFNLVTDVVYRFIDPRIAIGGKEATA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LN26</accession>
    <name>Q1LN26_CUPMC</name>
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        <submittedName>
            <fullName evidence="4">Putative transcriptional regulator, TetR-family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Rmet_1567</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
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        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08450.1"/>
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    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1567"/>
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        <property type="protein sequence ID" value="ABF08450"/>
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    </dbReference>
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        <property type="taxonomic scope" value="Bacteria"/>
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    <dbReference type="OrthoDB" id="1437290at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
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        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
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        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
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        <property type="entry name" value="HTH_TetR"/>
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    </dbReference>
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        <property type="entry name" value="SSF46689"/>
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    </dbReference>
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        <property type="entry name" value="HTH_TETR_2"/>
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    </dbReference>
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        <location>
            <begin position="40"/>
            <end position="100"/>
        </location>
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        <location>
            <begin position="63"/>
            <end position="82"/>
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    </feature>
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        <location>
            <begin position="1"/>
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        </location>
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        <location>
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            <end position="261"/>
        </location>
    </feature>
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        <location>
            <begin position="13"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF08450.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="261" mass="28636" checksum="B04226F81A6CA9CB" modified="2006-05-30" version="1">MLDVPIGSAKASPERSDRSDRQTDATPPKNRRLALQARSQATEKALLDAGLAHFEAHGFEGLSMQAVAEYAGTSIGALYFRFGDREGFIQAVLERGFDQIRQDTDDLLAKVTTEHASPHEVINAFVDLAVRVQKKSHGVFRAVLQRALVDPKAWEPVGRLGNDATRRLVSALSRFPEVVAIPDWEARLMFGVYAARSAQFNAMYNSQAPLPKGHVAMVHVLYELIVSYLGLPPIGRVGQGAKDQGGRPRKLATSSRTRHTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LN45</accession>
    <name>Q1LN45_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Conjugal transfer protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">traG</name>
        <name evidence="5" type="ordered locus">Rmet_1548</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the VirD4/TraG family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08431.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1548"/>
    <dbReference type="EnsemblBacteria" id="ABF08431">
        <property type="protein sequence ID" value="ABF08431"/>
        <property type="gene ID" value="Rmet_1548"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1548"/>
    <dbReference type="eggNOG" id="COG3505">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012039_0_1_4"/>
    <dbReference type="OMA" id="FRWTLDP"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
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        <property type="entry name" value="TraG/VirD4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02534">
        <property type="entry name" value="T4SS-DNA_transf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="72"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="572"/>
            <end position="613"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABF08431.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="664" mass="72738" checksum="6CA6780CAEEE2A48" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LNE3</accession>
    <name>Q1LNE3_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">23S rRNA methyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">rlmB</name>
        <name evidence="5" type="ordered locus">Rmet_1450</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08333.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516199.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1450"/>
    <dbReference type="EnsemblBacteria" id="ABF08333">
        <property type="protein sequence ID" value="ABF08333"/>
        <property type="gene ID" value="Rmet_1450"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1450"/>
    <dbReference type="eggNOG" id="COG0566">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021322_3_2_4"/>
    <dbReference type="OMA" id="ILIFEAK"/>
    <dbReference type="OrthoDB" id="1697536at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008173">
        <property type="term" value="F:RNA methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006396">
        <property type="term" value="P:RNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1280.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029028">
        <property type="entry name" value="Alpha/beta_knot_MTases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029064">
        <property type="entry name" value="L30e-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001537">
        <property type="entry name" value="SpoU_MeTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029026">
        <property type="entry name" value="tRNA_m1G_MTases_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00588">
        <property type="entry name" value="SpoU_methylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55315">
        <property type="entry name" value="SSF55315"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75217">
        <property type="entry name" value="SSF75217"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 5" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="SpoU_methylase" evidence="4">
        <location>
            <begin position="116"/>
            <end position="251"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABF08333.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="262" mass="27583" checksum="A8CA20596631FDDE" modified="2006-05-30" version="1">MKHVTSRDNALFKQLKALTGSTHQRRKAGQSVLDGIHLAQAYVAALGQPASCVVSERHYDHAEVRALLEKIDSDKLVVLADALFAQISSVVNGVDLMLVIDTPAGHLPATIDTDCIILDGLQDAGNVGSILRSAAAAGIPHAFLTKGCAFAWSVKTLRAGMGANFHLNIVEHCTLEMLESRLAVPLLATSSHADAAVFDTDLRQPVGWVIGNEGGGVSPEWMAHVSRTVGIPQPGGLESLNVAAATAVCLFEAVRQRRASGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LNG2</accession>
    <name>Q1LNG2_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transcriptional regulator, XRE family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_1431</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08314.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1431"/>
    <dbReference type="EnsemblBacteria" id="ABF08314">
        <property type="protein sequence ID" value="ABF08314"/>
        <property type="gene ID" value="Rmet_1431"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1431"/>
    <dbReference type="eggNOG" id="COG1396">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_057862_2_0_4"/>
    <dbReference type="OMA" id="VYHAEPG"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00093">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001387">
        <property type="entry name" value="Cro/C1-type_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010982">
        <property type="entry name" value="Lambda_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041413">
        <property type="entry name" value="MLTR_LBD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17765">
        <property type="entry name" value="MLTR_LBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00530">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47413">
        <property type="entry name" value="SSF47413"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH cro/C1-type" evidence="1">
        <location>
            <begin position="31"/>
            <end position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF08314.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="303" mass="33786" checksum="86700B5A86A982F8" modified="2006-05-30" version="1">MNARQEPGVLLDVDGYEEIEGTGREPELGAFLRARRESLDPARLGLSRMGRRRTPGLRREEVAAMADIGITWYTKLEQGRPIRVSPKVLNAVAAALQCSPSETEHLFTLAGMQRPAPLAGTSVCETVSATTQRVLDQLDPIPALVQNARFDIVAHNEAYCRLLGVDLPGLPVEDRNCIYLALTHPAWRAIVVDWHGMVANMVALYRAAMAEHVHDPVWDAQLKRYMAVSALFRDLWQRYQVRGIQNHVKQFTHPETGAFNLTQTNWWTAPRNGARLLVYVPADESAEEALHKLRPVTGQSAGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="99" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LNI3</accession>
    <name>Q1LNI3_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Putative peptide transporter permease subunit: membrane component of ABC superfamily</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">yliD</name>
        <name evidence="7" type="ordered locus">Rmet_1410</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cell membrane</location>
            <topology evidence="5">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the binding-protein-dependent transport system permease family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08293.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516165.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1410"/>
    <dbReference type="EnsemblBacteria" id="ABF08293">
        <property type="protein sequence ID" value="ABF08293"/>
        <property type="gene ID" value="Rmet_1410"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1410"/>
    <dbReference type="eggNOG" id="COG1173">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028518_1_1_4"/>
    <dbReference type="OMA" id="AWWLTTF"/>
    <dbReference type="OrthoDB" id="1734230at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025966">
        <property type="entry name" value="OppC_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12911">
        <property type="entry name" value="OppC_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="SSF161098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="35"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="99"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="136"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="163"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="218"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="263"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="6">
        <location>
            <begin position="97"/>
            <end position="286"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABF08293.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="300" mass="32294" checksum="60A4745E76D1B775" modified="2006-05-30" version="1">MSDISTPAAPEAASVPEAVRTPWTEFWRKFRKQHLALAAGAFVLLLVVVAILAPHIVPYDPENFFDYDALNAGPSAAHWMGVDSLGRDIFSRILAGTRISLAAGFFSVIIGAVIGTVLGLLAGYYEGWWDRIVTRICDVLFAFPGILLAIAIVAILGNGMVNVIFAVAVFSIPAFARLVRGNTLMLKRLTYVEAARSIGASDWTILMRHILPGTVSSIVVYFSMRIGTSIITAAGLSFLGLGAQPPTPEWGAMLNEARADMVTAPHVAIFPSLAIFLTVLAFNLLGDGLRDALDPKIDRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="96" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LNN1</accession>
    <name>Q1LNN1_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Acetyl-CoA acetyltransferase</fullName>
            <ecNumber evidence="7">2.3.1.9</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">bktB</name>
        <name evidence="7" type="ordered locus">Rmet_1362</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the thiolase-like superfamily. Thiolase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.9" evidence="7"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08245.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516125.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1362"/>
    <dbReference type="EnsemblBacteria" id="ABF08245">
        <property type="protein sequence ID" value="ABF08245"/>
        <property type="gene ID" value="Rmet_1362"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1362"/>
    <dbReference type="eggNOG" id="COG0183">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031026_0_0_4"/>
    <dbReference type="OMA" id="GIWEINE"/>
    <dbReference type="OrthoDB" id="1058688at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003985">
        <property type="term" value="F:acetyl-CoA C-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd00751">
        <property type="entry name" value="thiolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.47.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002155">
        <property type="entry name" value="Thiolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016039">
        <property type="entry name" value="Thiolase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020615">
        <property type="entry name" value="Thiolase_acyl_enz_int_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020610">
        <property type="entry name" value="Thiolase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020617">
        <property type="entry name" value="Thiolase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020616">
        <property type="entry name" value="Thiolase_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02803">
        <property type="entry name" value="Thiolase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00108">
        <property type="entry name" value="Thiolase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000429">
        <property type="entry name" value="Ac-CoA_Ac_transf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53901">
        <property type="entry name" value="SSF53901"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01930">
        <property type="entry name" value="AcCoA-C-Actrans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00098">
        <property type="entry name" value="THIOLASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00099">
        <property type="entry name" value="THIOLASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4 7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Thiolase_N" evidence="5">
        <location>
            <begin position="5"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="domain" description="Thiolase_C" evidence="6">
        <location>
            <begin position="271"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="active site" description="Acyl-thioester intermediate" evidence="3">
        <location>
            <position position="90"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="3">
        <location>
            <position position="350"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="3">
        <location>
            <position position="380"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR000429-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF02803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABF08245.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="394" mass="40995" checksum="2DF9BD0D9B5B250E" modified="2006-05-30" version="1">MAREVVVVSGVRTAIGTFGGSLKDQAPTDMGALVVREALARAGVSGDDVGHVVFGNVIQTEPKDMYLGRVAAVNGGVSINAPALTVNRLCGSGLQAIVSAAQTILLGDADVAIGGGAESMSRAPYLAPMARWGARMGDAKLVDMMLGALHDPFHAIHMGVTAENVAKEYDISRQQQDEAALESHRRASAAIKAGYFKDQIVPVTLKSRKGDVVFDTDEHVRHDATMDDMTKLKPVFVKENGTVTAGNASGLNDAAAAVVLMERAEAERRGLKPLARLVSYGHAGVDPKTMGIGPVPATKIALERAGLTVKDLDVIEANEAFAAQACAVTKALGLDPAKVNPNGSGISLGHPIGATGALITVKALYELQRVQGRYALVTMCIGGGQGIAAIFERV</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-10-07" version="64" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LNY1</accession>
    <name>Q1LNY1_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_1260</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08145.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1260"/>
    <dbReference type="EnsemblBacteria" id="ABF08145">
        <property type="protein sequence ID" value="ABF08145"/>
        <property type="gene ID" value="Rmet_1260"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1260"/>
    <dbReference type="eggNOG" id="COG3909">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_140302_0_0_4"/>
    <dbReference type="OMA" id="CHSAFRD"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010980">
        <property type="entry name" value="Cyt_c/b562"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002321">
        <property type="entry name" value="Cyt_c_II"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01322">
        <property type="entry name" value="Cytochrom_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47175">
        <property type="entry name" value="SSF47175"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51009">
        <property type="entry name" value="CYTCII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193601" evidence="1">
        <location>
            <begin position="34"/>
            <end position="153"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF08145.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="153" mass="16654" checksum="7A0A77B8B6C1A4D9" modified="2006-05-30" version="1">MNQPLKPRSFFTVSLSVLLATFATALGAQSVEAAKPMALRTIMERLGRDMQAVTGAISKEDWPLVAELAPRIAKHAEPPMSEKMRILAWLGADAGKFRGLDGQVHDAATAMGEAAQRNDGQAVIAAFSKTQQSCLACHQSYRRSFVEKFYGSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-10-07" version="52" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LPD5</accession>
    <name>Q1LPD5_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative membrane protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_1105</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07991.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1105"/>
    <dbReference type="EnsemblBacteria" id="ABF07991">
        <property type="protein sequence ID" value="ABF07991"/>
        <property type="gene ID" value="Rmet_1105"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1105"/>
    <dbReference type="eggNOG" id="ENOG5033AQ3">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1873723_0_0_4"/>
    <dbReference type="OMA" id="IRNANHY"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022109">
        <property type="entry name" value="DUF3649"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12365">
        <property type="entry name" value="DUF3649"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="54"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="88"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="113"/>
            <end position="133"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF07991.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="136" mass="14869" checksum="C5B16DE28BFABA2E" modified="2006-05-30" version="1">MSYPTYNPRSSFPTQIRNANHYHMRLCLLGLNGSRRIMKQRVSVGYRMAIGSRVIAAVVGGYAVAALCTGCLSLALVRWTDVARAEAVMTATLMSFLWFALAVIWVFAASTAWRAWVGLCVPGVLMAAGWLILRGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LPY9</accession>
    <name>Q1LPY9_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Transcriptional regulator, TetR/AcrR-family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_0901</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07787.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515727.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0901"/>
    <dbReference type="EnsemblBacteria" id="ABF07787">
        <property type="protein sequence ID" value="ABF07787"/>
        <property type="gene ID" value="Rmet_0901"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0901"/>
    <dbReference type="eggNOG" id="COG1309">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_091262_0_0_4"/>
    <dbReference type="OMA" id="YHYPAKD"/>
    <dbReference type="OrthoDB" id="1437290at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001647">
        <property type="entry name" value="HTH_TetR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025722">
        <property type="entry name" value="TetR"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13972">
        <property type="entry name" value="TetR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00440">
        <property type="entry name" value="TetR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00455">
        <property type="entry name" value="HTHTETR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50977">
        <property type="entry name" value="HTH_TETR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH tetR-type" evidence="2">
        <location>
            <begin position="10"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="H-T-H motif" evidence="1">
        <location>
            <begin position="33"/>
            <end position="52"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF07787.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="236" mass="27878" checksum="9F83BA231E07156F" modified="2006-05-30" version="1">MEAKPQAGPRRTRDRILDVSLRLFNELGEPNVTTTTIAEAMEISPGNLYYHFRNKDDIINSIFVRFEQEMERRLKMPEDHKATLGESWGYLQYMSEFLWNYRFLYRDINDLLARNRMLETNFKRIVDQKKRFALEICRQFQEDGDMDATPEQVDAICTNIVVIATYWLSFQFVQHPRQYNDPEQIRGYLHGSSYHIFSILAPYLRGKAREAFDQLAREYAAEKAAADAAKLEKDRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LQ80</accession>
    <name>Q1LQ80_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">NADH pyrophosphatase I of the Nudix family of hydrolases</fullName>
            <ecNumber evidence="3">3.6.1.22</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_0810</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EC" id="3.6.1.22" evidence="3"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07696.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515644.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0810"/>
    <dbReference type="EnsemblBacteria" id="ABF07696">
        <property type="protein sequence ID" value="ABF07696"/>
        <property type="gene ID" value="Rmet_0810"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0810"/>
    <dbReference type="eggNOG" id="COG2816">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037162_16_2_4"/>
    <dbReference type="OMA" id="IIAYHVK"/>
    <dbReference type="OrthoDB" id="1846332at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000210">
        <property type="term" value="F:NAD+ diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015797">
        <property type="entry name" value="NUDIX_hydrolase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020084">
        <property type="entry name" value="NUDIX_hydrolase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000086">
        <property type="entry name" value="NUDIX_hydrolase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029401">
        <property type="entry name" value="Nudix_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00293">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14803">
        <property type="entry name" value="Nudix_N_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55811">
        <property type="entry name" value="SSF55811"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51462">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00893">
        <property type="entry name" value="NUDIX_BOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nudix hydrolase" evidence="2">
        <location>
            <begin position="38"/>
            <end position="159"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF07696.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="198" mass="22563" checksum="F8721246F8279846" modified="2006-05-30" version="1">MHNYRFCPQCGSELEHLPMSGRYRHVCLRDGCGFVHWDNPLPVLAAVVEYEGKLLLARNAVWPEKMFALVTGFMERDETPELGVARELKEETNLDTVSASLIGVYDFMRKNELIIAYHVQATGNIQLSEELAEYKLVAPEKMRIWNAGTGFAVADFLERRGMPVRFYDRATGDDIPDPRRPTDFSLLGASLQYGIYQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LQJ1</accession>
    <name>Q1LQJ1_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Transcriptional regulator, LysR-family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_0699</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the LysR transcriptional regulatory family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07585.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008643631.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0699"/>
    <dbReference type="EnsemblBacteria" id="ABF07585">
        <property type="protein sequence ID" value="ABF07585"/>
        <property type="gene ID" value="Rmet_0699"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0699"/>
    <dbReference type="eggNOG" id="COG0583">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039613_6_3_4"/>
    <dbReference type="OMA" id="ANRDELC"/>
    <dbReference type="OrthoDB" id="990666at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd08415">
        <property type="entry name" value="PBP2_LysR_opines_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005119">
        <property type="entry name" value="LysR_subst-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037424">
        <property type="entry name" value="NocR_PBP2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000847">
        <property type="entry name" value="Tscrpt_reg_HTH_LysR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00126">
        <property type="entry name" value="HTH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03466">
        <property type="entry name" value="LysR_substrate"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00039">
        <property type="entry name" value="HTHLYSR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50931">
        <property type="entry name" value="HTH_LYSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH lysR-type" evidence="2">
        <location>
            <begin position="1"/>
            <end position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF07585.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="317" mass="34833" checksum="5813E511653AACD6" modified="2006-05-30" version="1">MRLRQIEVFRAVMLTGTVSEAARLLHVSQPVVSRVLQHAESSLGFRLFDRQRGRLQPTPEALALYGDVERLYGEIERVRRVSESLRHKGTGRLRVAATPSLANPLLVPAVRRFCARHPETQVQVLTHHTSEIVEALLANQIDVGFAFSPPRHEAITSEPVAAGRMLLAVPRPQALPSGGRRGIVSIRKLMERPFIGYDDDSSLGLLVRQTLARHALEPHSTLEVQTYSLALALVDAGLGVTLLDQYTALSANPANVALHDVQPELPFEIQMLRATHRGKSSLVDDMRTQFAAAASALAATLDDRLDSPVVQFDPAPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="62" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LQV9</accession>
    <name>Q1LQV9_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized paraquat-inducible protein A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">pqiA2</name>
        <name evidence="2" type="ordered locus">Rmet_0581</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07467.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515442.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0581"/>
    <dbReference type="EnsemblBacteria" id="ABF07467">
        <property type="protein sequence ID" value="ABF07467"/>
        <property type="gene ID" value="Rmet_0581"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0581"/>
    <dbReference type="eggNOG" id="COG2995">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041903_1_1_4"/>
    <dbReference type="OMA" id="WGMLEVY"/>
    <dbReference type="OrthoDB" id="1604315at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007498">
        <property type="entry name" value="PqiA-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04403">
        <property type="entry name" value="PqiA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="62"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="109"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="185"/>
            <end position="203"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF07467.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="229" mass="25389" checksum="CA292533E9D5E6E4" modified="2006-05-30" version="1">MPEHHGPAASASAPAPDLRHLVACEYCDALYERASLAPGERANCLRCGSELYRESTRAYRRLLPLVFTALILFVISNTYPIVEMDLKGVRTETTLWGAVQALYSDQMTLVAMLVFATTILFPLSEMLMMCYLLVPMAQRKMPHGFDAIVRGIRQTRPWGMIEVFMIGVLVTLVKLSTMAEVLPGVALWSFGALVVVLAAMLSFDPRDLWHYLETPDGDDTPPGAQEPTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LQW2</accession>
    <name>Q1LQW2_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">ABC_trans_aux domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_0578</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07464.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515439.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0578"/>
    <dbReference type="EnsemblBacteria" id="ABF07464">
        <property type="protein sequence ID" value="ABF07464"/>
        <property type="gene ID" value="Rmet_0578"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0578"/>
    <dbReference type="eggNOG" id="COG3009">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_096001_0_0_4"/>
    <dbReference type="OMA" id="NERWTST"/>
    <dbReference type="OrthoDB" id="1956076at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005586">
        <property type="entry name" value="ABC_trans_aux"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03886">
        <property type="entry name" value="ABC_trans_aux"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193365" description="ABC_trans_aux domain-containing protein" evidence="1">
        <location>
            <begin position="21"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="domain" description="ABC_trans_aux" evidence="2">
        <location>
            <begin position="27"/>
            <end position="187"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF03886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF07464.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="195" mass="20774" checksum="C1D2EE8F923600CB" modified="2006-05-30" version="1">MMKRSLMLLALAAALLNGCASPEPRYYTLASTAAPAATPPTPGTQPIWIEVSPVRVPERLNRTQLVVADGNAGVVKMLDLSRWSAPLPDELRDALSQQLQAKLGAVDIYQQGLSEVEPVYRVTTEVVRLDADPGQRASASIAWTVRRLPDGKVLGGRTEAELPAPGQVDGVVNAYRQIVAATAADIAAGVLSLRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LR01</accession>
    <name>Q1LR01_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 6">Superoxide dismutase</fullName>
            <ecNumber evidence="2 6">1.15.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">sodB</name>
        <name evidence="9" type="ordered locus">Rmet_0539</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Destroys radicals which are normally produced within the cells and which are toxic to biological systems.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>2 H(+) + 2 superoxide = H2O2 + O2</text>
            <dbReference type="Rhea" id="RHEA:20696"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:18421"/>
            <dbReference type="EC" id="1.15.1.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the iron/manganese superoxide dismutase family.</text>
    </comment>
    <dbReference type="EC" id="1.15.1.1" evidence="2 6"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07425.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008640957.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0539"/>
    <dbReference type="EnsemblBacteria" id="ABF07425">
        <property type="protein sequence ID" value="ABF07425"/>
        <property type="gene ID" value="Rmet_0539"/>
    </dbReference>
    <dbReference type="GeneID" id="60824136"/>
    <dbReference type="KEGG" id="rme:Rmet_0539"/>
    <dbReference type="eggNOG" id="COG0605">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031625_0_0_4"/>
    <dbReference type="OMA" id="KWGSFDK"/>
    <dbReference type="OrthoDB" id="1440645at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004784">
        <property type="term" value="F:superoxide dismutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.990">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.55.40.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001189">
        <property type="entry name" value="Mn/Fe_SOD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019833">
        <property type="entry name" value="Mn/Fe_SOD_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019832">
        <property type="entry name" value="Mn/Fe_SOD_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019831">
        <property type="entry name" value="Mn/Fe_SOD_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036324">
        <property type="entry name" value="Mn/Fe_SOD_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036314">
        <property type="entry name" value="SOD_C_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02777">
        <property type="entry name" value="Sod_Fe_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00081">
        <property type="entry name" value="Sod_Fe_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000349">
        <property type="entry name" value="SODismutase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01703">
        <property type="entry name" value="MNSODISMTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46609">
        <property type="entry name" value="SSF46609"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54719">
        <property type="entry name" value="SSF54719"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00088">
        <property type="entry name" value="SOD_MN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 6 9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Sod_Fe_N" evidence="7">
        <location>
            <begin position="2"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="domain" description="Sod_Fe_C" evidence="8">
        <location>
            <begin position="89"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="27"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="74"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="157"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="161"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR000349-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000414"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ABF07425.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="192" mass="21527" checksum="FF71BAF5E0996CC0" modified="2006-05-30" version="1">MEHKLPSLPYAHDALAPHISKETLEFHHDKHHQTYVTNLNNLIKGTEFESASLEEIVKKSSGGIFNNAAQVWNHTFYWESMKPNGGGQPTGALADAINAKWGSFDKFKEEFTKVAVGTFGSGWAWLVKKADGSLDLVSTSNAATPLTTDAKPLLTCDVWEHAYYIDYRNARPKYVEAFWNVVSWDFASKNFA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="111" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LR75</accession>
    <name>Q1LR75_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">DNA-binding response regulator in two-component regulatory system with Rmet_0464</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Rmet_0465</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07351.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515340.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0465"/>
    <dbReference type="EnsemblBacteria" id="ABF07351">
        <property type="protein sequence ID" value="ABF07351"/>
        <property type="gene ID" value="Rmet_0465"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0465"/>
    <dbReference type="eggNOG" id="COG0745">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_30_1_4"/>
    <dbReference type="OMA" id="LMREVWD"/>
    <dbReference type="OrthoDB" id="1020672at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00383">
        <property type="entry name" value="trans_reg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001867">
        <property type="entry name" value="OmpR/PhoB-type_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039420">
        <property type="entry name" value="WalR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48111">
        <property type="entry name" value="PTHR48111"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00486">
        <property type="entry name" value="Trans_reg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00862">
        <property type="entry name" value="Trans_reg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51755">
        <property type="entry name" value="OMPR_PHOB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Response regulatory" evidence="3">
        <location>
            <begin position="2"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="domain" description="OmpR/PhoB-type" evidence="4">
        <location>
            <begin position="124"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="OmpR/PhoB-type" evidence="2">
        <location>
            <begin position="124"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="1">
        <location>
            <position position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABF07351.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="229" mass="24987" checksum="77C166B4ED5CA43F" modified="2006-05-30" version="1">MRILIAEDDDVLADGLTRSLRQSGYAVDHVANGVEADSALSTQSFDLLILDLGLPRMSGLEVLKRLRARSAMVPVLILTAADSVDERVKGLDLGADDYMAKPFALSELEARVRALVRRGAGGGATLMRHGPLAFDQVGRIAYLNEQMLDLSAREVGLLEILLTRSGRLVSKEQLVDHLCEWGEEVSNNAIEVYVHRLRKKIEVGGIRIATVRGLGYCLEKYQPAVAAHG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-11-28" modified="2022-05-25" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LRD0</accession>
    <name>RL13_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">50S ribosomal protein L13</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rplM</name>
        <name type="ordered locus">Rmet_0410</name>
    </gene>
    <organism>
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">This protein is one of the early assembly proteins of the 50S ribosomal subunit, although it is not seen to bind rRNA by itself. It is important during the early stages of 50S assembly.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the 50S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL13 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07296.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008646003.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q1LRD0"/>
    <dbReference type="SMR" id="Q1LRD0"/>
    <dbReference type="STRING" id="266264.Rmet_0410"/>
    <dbReference type="EnsemblBacteria" id="ABF07296">
        <property type="protein sequence ID" value="ABF07296"/>
        <property type="gene ID" value="Rmet_0410"/>
    </dbReference>
    <dbReference type="GeneID" id="60824012"/>
    <dbReference type="KEGG" id="rme:Rmet_0410"/>
    <dbReference type="eggNOG" id="COG0102">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_082184_2_2_4"/>
    <dbReference type="OMA" id="DFVVIIN"/>
    <dbReference type="OrthoDB" id="1822215at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00392">
        <property type="entry name" value="Ribosomal_L13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1180.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01366">
        <property type="entry name" value="Ribosomal_L13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005822">
        <property type="entry name" value="Ribosomal_L13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005823">
        <property type="entry name" value="Ribosomal_L13_bac-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036899">
        <property type="entry name" value="Ribosomal_L13_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11545">
        <property type="entry name" value="PTHR11545"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00572">
        <property type="entry name" value="Ribosomal_L13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002181">
        <property type="entry name" value="Ribosomal_L13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52161">
        <property type="entry name" value="SSF52161"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01066">
        <property type="entry name" value="rplM_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <feature type="chain" id="PRO_0000261779" description="50S ribosomal protein L13">
        <location>
            <begin position="1"/>
            <end position="142"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01366"/>
        </source>
    </evidence>
    <sequence length="142" mass="16041" checksum="CE7773FD5362BCF5" modified="2006-05-30" version="1">MKTFSAKPAEVKRDWYVIDATDKVLGRVASEVARRLRGKHKPEFTPHVDTGDFIIIVNAAKLRVTGTKEQDKKYYRHSGYPGGIYETTFGKMQQRFPGRALEKAVKGMLPKGPLGYAMIKKLKVYAEAEHPHSAQQPKVLEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="70" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LRD7</accession>
    <name>Q1LRD7_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Glutamine amidotransferases class-II</fullName>
            <ecNumber evidence="2">2.4.2.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">yafJ</name>
        <name evidence="2" type="ordered locus">Rmet_0403</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.4.2.-" evidence="2"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07289.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515279.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0403"/>
    <dbReference type="EnsemblBacteria" id="ABF07289">
        <property type="protein sequence ID" value="ABF07289"/>
        <property type="gene ID" value="Rmet_0403"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0403"/>
    <dbReference type="eggNOG" id="COG0121">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_059273_0_0_4"/>
    <dbReference type="OMA" id="YWVFAHN"/>
    <dbReference type="OrthoDB" id="693464at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006541">
        <property type="term" value="P:glutamine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026869">
        <property type="entry name" value="EgtC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017932">
        <property type="entry name" value="GATase_2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13230">
        <property type="entry name" value="GATase_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="SSF56235"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51278">
        <property type="entry name" value="GATASE_TYPE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="2" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glutamine amidotransferase type-2" evidence="1">
        <location>
            <begin position="2"/>
            <end position="251"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF07289.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="276" mass="30475" checksum="AAD73BC73158E2E4" modified="2006-05-30" version="1">MCQLLGMNCATPTDVTFSFTGFAARGGVTDHHSDGFGVAFFEDKACRLFIDNQSAGTSPVADLIKRYPIKSKNVISHIRKATQGTVLLENCHPFMRELWGRHWIFAHNGDLHGFAPFLSGVYQPVGDTDSELAFCALMQGLRKRFPGSQPPLNELGHALADITRDITLHGVFNFLLCNGQALFAHCSTRLYYIVRQWPFSKAHLIDADLSIDFAQVTTPDDRVAVIATAPLTDNETWTQFAPGELIMFEDGRPTMTWSVPIPPEVQAKNAANTACT</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LRE3</accession>
    <name>Q1LRE3_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Transcriptional regulator, LysR-family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Rmet_0397</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the LysR transcriptional regulatory family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07283.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0397"/>
    <dbReference type="EnsemblBacteria" id="ABF07283">
        <property type="protein sequence ID" value="ABF07283"/>
        <property type="gene ID" value="Rmet_0397"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0397"/>
    <dbReference type="eggNOG" id="COG0583">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039613_4_1_4"/>
    <dbReference type="OMA" id="WHVPLEI"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005119">
        <property type="entry name" value="LysR_subst-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000847">
        <property type="entry name" value="Tscrpt_reg_HTH_LysR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00126">
        <property type="entry name" value="HTH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03466">
        <property type="entry name" value="LysR_substrate"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00039">
        <property type="entry name" value="HTHLYSR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50931">
        <property type="entry name" value="HTH_LYSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH lysR-type" evidence="3">
        <location>
            <begin position="16"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="298"/>
            <end position="317"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF07283.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="329" mass="36970" checksum="7A2983BBE5814CAB" modified="2006-05-30" version="1">MRAVRPGGRRVIIRAMEIKWLEDFVSLAETHSFSRSAELRHVTQPAFSRRIQSLEAWVGTELIDRSSYPTSLTAAGKVFYEQALAMLAQVSETRALMRGQRSANAQVLEFAVPHTLSLTFFPEWLKGVERKVGTLPCRLRALNVHDAVLMLVEGGCDLVMVYHHSRQAIQLDPAHYDMLVLGTERLSPYSVPDASGRPLFRMPGTDKKPVPYLSYTPNAFLGRMVDMLLAESPETLKLDKCYETDMAEALKVMALAGHGMAFLPESAVREDVAAGRLVRADAAKGLPQSIDMEIRLYRERPPEHANERRGSQQRRKRQLVDKIWAALTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LSD9</accession>
    <name>Q1LSD9_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Cell shape-determining protein MreB</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2 3" type="primary">mreB</name>
        <name evidence="3" type="ordered locus">Rmet_0051</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Forms membrane-associated dynamic filaments that are essential for cell shape determination. Acts by regulating cell wall synthesis and cell elongation, and thus cell shape. A feedback loop between cell geometry and MreB localization may maintain elongated cell shape by targeting cell wall growth to regions of negative cell wall curvature.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Forms polymers.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <text evidence="2">Membrane-associated.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the FtsA/MreB family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF06937.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008648066.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0051"/>
    <dbReference type="EnsemblBacteria" id="ABF06937">
        <property type="protein sequence ID" value="ABF06937"/>
        <property type="gene ID" value="Rmet_0051"/>
    </dbReference>
    <dbReference type="GeneID" id="60822770"/>
    <dbReference type="KEGG" id="rme:Rmet_0051"/>
    <dbReference type="eggNOG" id="COG1077">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_052037_0_0_4"/>
    <dbReference type="OMA" id="MVICIPS"/>
    <dbReference type="OrthoDB" id="410781at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000902">
        <property type="term" value="P:cell morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008360">
        <property type="term" value="P:regulation of cell shape"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd10225">
        <property type="entry name" value="MreB_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02207">
        <property type="entry name" value="MreB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004753">
        <property type="entry name" value="MreB"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01652">
        <property type="entry name" value="SHAPEPROTEIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="SSF53067"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00904">
        <property type="entry name" value="mreB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0133">Cell shape</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="19"/>
            <end position="21"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="169"/>
            <end position="171"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="217"/>
            <end position="220"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="297"/>
            <end position="300"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02207"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF06937.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="347" mass="37091" checksum="9AE07FF9CA20DAA7" modified="2006-05-30" version="1">MFGFLRSYFSNDLAIDLGTANTLIYMRDKGIVLDEPSVVAIRQEGGPNAKKTITAVGKEAKQMLGKVPGNIEAIRPMKDGVIADFTVTEQMLKQFIKMVHDSKLLRPSPRIIICVPCGSTQVERRAIRESALGAGASQVYLIEEPMSAAIGAGLPVSEPSGSMVVDIGGGTTEVGIISLGGMVYKGSVRVGGDKFDEAIVNYIRRNYGMLIGEQTAEAIKKEIGSAFPGSEVREMEVKGRNLSEGIPRAFTVSSNEILEALTDPLNQIVSAVKIALEQTPPELGADIAERGMMLTGGGALLRDLDRLLAEETGLPVLVAEDPLTCVVRGSGMALERMDKLGSIFSYE</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LSH0</accession>
    <name>Q1LSH0_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Fumarylacetoacetate hydrolase family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_0020</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF06906.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011514954.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0020"/>
    <dbReference type="EnsemblBacteria" id="ABF06906">
        <property type="protein sequence ID" value="ABF06906"/>
        <property type="gene ID" value="Rmet_0020"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0020"/>
    <dbReference type="eggNOG" id="COG0179">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028458_3_4_4"/>
    <dbReference type="OMA" id="YVSQCMS"/>
    <dbReference type="OrthoDB" id="775108at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.850.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011234">
        <property type="entry name" value="Fumarylacetoacetase-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036663">
        <property type="entry name" value="Fumarylacetoacetase_C_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01557">
        <property type="entry name" value="FAA_hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56529">
        <property type="entry name" value="SSF56529"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAA_hydrolase" evidence="1">
        <location>
            <begin position="71"/>
            <end position="276"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF06906.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="288" mass="30850" checksum="672D589CF550487F" modified="2006-05-30" version="1">MKLVRIGEPGAERPGMLDSDGRVRDLSGVIDNIGPQELAPGVLARLANIDPATLPVVEGGRFGVPWSGVGKIVAIGLNYADHAAEAGMPLPTEPIVFLKANTSLNGPNDNVMLPLDSQKSDWEVELGVVIGTRARNVWKEQALDYVAGYCVVNDVSEREFQLERGGTWDKGKGCDTFCPAGPWLVTRDEVPDAQKLGMWLDVNGERMQKGSTATMVFDVATIVSYVSRFMTLEPGDLIATGTPPGVGMGFKPPRYLRAGDVMRLGVEGLGEQSQNVVAYVGRGGAIRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-04-26" modified="2021-09-29" version="98" xmlns="http://uniprot.org/uniprot">
    <accession>Q58AF6</accession>
    <name>Q58AF6_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Permease major facilitator superfamily MFS_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Rmet_6139</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="7 8">
        <name>pMOL30</name>
    </geneLocation>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="8">Plasmid pMOL30</plasmid>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP000354">
        <property type="protein sequence ID" value="ABF12998.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008642705.1">
        <property type="nucleotide sequence ID" value="NC_007971.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_145662.1">
        <property type="nucleotide sequence ID" value="NC_006466.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF12998">
        <property type="protein sequence ID" value="ABF12998"/>
        <property type="gene ID" value="Rmet_6139"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6139"/>
    <dbReference type="HOGENOM" id="CLU_040020_2_0_4"/>
    <dbReference type="OMA" id="FGFHRMM"/>
    <dbReference type="OrthoDB" id="1345431at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid pMOL30"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="SSF103473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0614">Plasmid</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="50"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="96"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="196"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="243"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="268"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="308"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="337"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="399"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="domain" description="MFS" evidence="6">
        <location>
            <begin position="30"/>
            <end position="426"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABF12998.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="426" mass="44644" checksum="AAF2C79607F335A2" modified="2005-04-26" version="1">MTDSKDTAPLSNDKPPAAPVPLQREWLNRTVVGAGLTSALGDFCYETTTVILPGFLAILGIPAAVLGLIEGLADAVASFTKMISGYVADKLGHRKLLVLVGYALTPAGQIAIALATAWPMLLVGRIISWFGKGLRGPLRDAIVIQAIPPRARGRAFGFHRAMDTIGAVVGPLLGVALLGWAQSHHGWNDPIGPFRLVLWLSVIPGVLAVLAFLGFVKDPAFSPNPGLRFFRALRGLPPKFKRYLGAVGLFGMGDFSHSLLILGATKLLTPALGIVQAAQIAGGLYVWRNVVQSAASFPVGMLADRVGALRILVLGYALGAMTGLLMAITFWLHVNTLALLAAVFLLAGLYTAVQEALEPTVIAEMVSADTLAMSIGALGTTNGTAKFISSAGVGLIWSALSPVLGFAFATITMAAGTVMLARLRHI</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-01-04" modified="2022-08-03" version="123" xmlns="http://uniprot.org/uniprot">
    <accession>Q5NUY9</accession>
    <name>Q5NUY9_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Serine-based site-specific recombinase activity TnpR (Resolvase) Tn4378 mercury transposon Tn3 family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">tnpR</name>
        <name evidence="8" type="ordered locus">Rmet_6187</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="8 9">
        <name>pMOL28</name>
    </geneLocation>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="9">Plasmid pMOL28</plasmid>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the site-specific recombinase resolvase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000355">
        <property type="protein sequence ID" value="ABF13046.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011239984.1">
        <property type="nucleotide sequence ID" value="NC_007972.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_161723.1">
        <property type="nucleotide sequence ID" value="NC_006525.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF13046">
        <property type="protein sequence ID" value="ABF13046"/>
        <property type="gene ID" value="Rmet_6187"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6187"/>
    <dbReference type="HOGENOM" id="CLU_010686_8_3_4"/>
    <dbReference type="OMA" id="HLMADST"/>
    <dbReference type="OrthoDB" id="1791095at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid pMOL28"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000150">
        <property type="term" value="F:DNA strand exchange activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1390">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006118">
        <property type="entry name" value="Recombinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006119">
        <property type="entry name" value="Resolv_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036162">
        <property type="entry name" value="Resolvase-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006120">
        <property type="entry name" value="Resolvase_HTH_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02796">
        <property type="entry name" value="HTH_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00239">
        <property type="entry name" value="Resolvase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00857">
        <property type="entry name" value="Resolvase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53041">
        <property type="entry name" value="SSF53041"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00397">
        <property type="entry name" value="RECOMBINASES_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00398">
        <property type="entry name" value="RECOMBINASES_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51736">
        <property type="entry name" value="RECOMBINASES_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0229">DNA integration</keyword>
    <keyword evidence="4" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8" id="KW-0614">Plasmid</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Resolvase/invertase-type recombinase catalytic" evidence="7">
        <location>
            <begin position="4"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="active site" description="O-(5'-phospho-DNA)-serine intermediate" evidence="5 6">
        <location>
            <position position="12"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR606118-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51736"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ABF13046.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="186" mass="21198" checksum="297FE3ACF3C19C2A" modified="2005-01-04" version="1">MQGQRIGYVRVSSFDQNPERQLEHVEVGKVFTDKASGKDTQRPELDSLLAFVREGDTVVVHSMDRLARNLDDLRRLVQKLTKRGVRIEFVKESLTFTGEDSPMANLMLSVMGAFAEFERALIRERQREGIALAKQRGAYRGRKKALSPEQVADLRQRAAAGEQKAKLAREFGVSRETLYQYLRADQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-01-04" modified="2022-01-19" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>Q5NV02</accession>
    <name>Q5NV02_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Type II secretory pathway component PulF-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">pilR</name>
        <name evidence="2" type="ordered locus">Rmet_6290</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2 3">
        <name>pMOL28</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid pMOL28</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000355">
        <property type="protein sequence ID" value="ABF13149.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011239910.1">
        <property type="nucleotide sequence ID" value="NC_006525.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_161650.1">
        <property type="nucleotide sequence ID" value="NC_006525.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF13149">
        <property type="protein sequence ID" value="ABF13149"/>
        <property type="gene ID" value="Rmet_6290"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6290"/>
    <dbReference type="HOGENOM" id="CLU_056146_0_0_4"/>
    <dbReference type="OMA" id="MWRNRRD"/>
    <dbReference type="OrthoDB" id="1312986at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid pMOL28"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.81.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042094">
        <property type="entry name" value="T2SS_GspF_sf"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="150"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="209"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="366"/>
            <end position="384"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF13149.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="411" mass="46166" checksum="0D290C0BD9599BE6" modified="2005-01-04" version="1">MMQRLKDALAAIKRKKYAAAAESIGGATGRFPDKLWFAKLEFSPKRADYYYDLAMRIEKMPGEPISNHFAKDAARRAGEPLGLLAALWLARYEGLDGQTQESRLAEIFRGTVPDEDLAILAVAEQGGDVKDGLFRLAENLRAMSEAKSNILLLLASMGITLIILHVYLGVMAFIVAPMLDRSFANLLPVDGYGPIARAFHLGTTFLREWGWLVLLGEVGLVWWVLRALRNYTGPYRAWLDKNVIIFDFFRRFQGAQFLAAMSAVTQRFGGDMNNLTNALEMMRANAYPYLAQHIRRIEATLEESPNAGGKMFDTGLFDRDTTYRIQDIAEYEDDLSKMLNTVSVTVLKTAPRELEQRASKFNRRSSVLLIVLITALAFMPAFMARELKKSMQVHGIKTHQKSINPSTLPSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-03-23" modified="2020-10-07" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>D3DXU0</accession>
    <name>D3DXU0_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_6502</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ADC45110.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADC45110">
        <property type="protein sequence ID" value="ADC45110"/>
        <property type="gene ID" value="Rmet_6502"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6502"/>
    <dbReference type="HOGENOM" id="CLU_1359467_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADC45110.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="201" mass="21101" checksum="48B286140BEBE2AC" modified="2010-03-23" version="1">MSSISGMSNWLGAGEQFCHTALAMSCGTYRRHMATAQKREHIAAQLKEIPEKSDRQVAKALGVSHHTVSEERAALGAGGQIAHQPTRTGTKSRLVGDIVRVLELVQVISMGFGLAVSLRPINLYSLADDAVTALTGRGGTTPLLGYLVTQAGFAGQLVKVDVATTPCGCGGKIIAIGGVGSRYSRCQQQGEKGVCHSSILR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-03-23" modified="2020-10-07" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>D3DXU1</accession>
    <name>D3DXU1_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_6503</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ADC45111.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADC45111">
        <property type="protein sequence ID" value="ADC45111"/>
        <property type="gene ID" value="Rmet_6503"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6503"/>
    <dbReference type="HOGENOM" id="CLU_2938423_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADC45111.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="60" mass="6374" checksum="38195A2EBFEBB5F2" modified="2010-03-23" version="1">MNVCGEAIVPGSANVCVLCELPNAAQPEMTAAARTVKARLPVRAATPLRPRVEEGERFIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-03-23" modified="2021-06-02" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>D3DYD1</accession>
    <name>D3DYD1_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_6722</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ADC45301.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519771.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_6722"/>
    <dbReference type="EnsemblBacteria" id="ADC45301">
        <property type="protein sequence ID" value="ADC45301"/>
        <property type="gene ID" value="Rmet_6722"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6722"/>
    <dbReference type="HOGENOM" id="CLU_2864651_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADC45301.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="64" mass="7126" checksum="93EEC5209436B586" modified="2010-03-23" version="1">MPQLFKSFAMGERALSSRIAVAPLTPARAEPFVANQDLVAHLEHNWPRSVAPHAFHDVLRGLPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-03-23" modified="2020-10-07" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>D3DYE6</accession>
    <name>D3DYE6_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_6737</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ADC45316.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADC45316">
        <property type="protein sequence ID" value="ADC45316"/>
        <property type="gene ID" value="Rmet_6737"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6737"/>
    <dbReference type="HOGENOM" id="CLU_2900990_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADC45316.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="62" mass="7047" checksum="D1A81C12D9D13009" modified="2010-03-23" version="1">MVSVVDESLVRGDYQSSVLLMAAGSELSVRWPKELTQSLVAEASRQSQLFRYLFWLVVRRSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-03-23" modified="2021-06-02" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>D3DYJ5</accession>
    <name>D3DYJ5_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_6395</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="1 2">
        <name>pMOL28</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid pMOL28</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000355">
        <property type="protein sequence ID" value="ADC45365.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011514931.1">
        <property type="nucleotide sequence ID" value="NC_007972.2"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADC45365">
        <property type="protein sequence ID" value="ADC45365"/>
        <property type="gene ID" value="Rmet_6395"/>
    </dbReference>
    <dbReference type="GeneID" id="60824660"/>
    <dbReference type="KEGG" id="rme:Rmet_6395"/>
    <dbReference type="HOGENOM" id="CLU_2737171_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid pMOL28"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADC45365.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="71" mass="8623" checksum="AF5F4848238E7CAB" modified="2010-03-23" version="1">MMQKFELWEFFNEKGNSYSVELCEEGKFVNLDPPEWKKPRLLKVFEARDIDEATQMRNDYMGWGKHYPTKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="54" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LA34</accession>
    <name>Q1LA34_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">SilD transmembrane protein involved in copper, silver resistance</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">silD</name>
        <name evidence="1" type="ordered locus">Rmet_6133</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="1 2">
        <name>pMOL30</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid pMOL30</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000354">
        <property type="protein sequence ID" value="ABF12992.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008642716.1">
        <property type="nucleotide sequence ID" value="NC_007971.2"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF12992">
        <property type="protein sequence ID" value="ABF12992"/>
        <property type="gene ID" value="Rmet_6133"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6133"/>
    <dbReference type="HOGENOM" id="CLU_121434_0_0_4"/>
    <dbReference type="OMA" id="LCLAHCQ"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid pMOL30"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF12992.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="131" mass="14020" checksum="8CAADFD76FC6B816" modified="2006-05-30" version="1">MHASYRRHWIAAFLLISFLTVQLAAAAYACEGSRYALSLTASMTGMAESCPDMAIKRGHSHAGLCQAHCHQDSKSADHASPQLPVFQAASVSPVATPDLTPILGGYRLATGNPVPRPPPRPLAILHCCFRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LA88</accession>
    <name>Q1LA88_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_6079</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="1 2">
        <name>pMOL30</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid pMOL30</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000354">
        <property type="protein sequence ID" value="ABF12938.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011514862.1">
        <property type="nucleotide sequence ID" value="NC_007971.2"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF12938">
        <property type="protein sequence ID" value="ABF12938"/>
        <property type="gene ID" value="Rmet_6079"/>
    </dbReference>
    <dbReference type="GeneID" id="60825042"/>
    <dbReference type="KEGG" id="rme:Rmet_6079"/>
    <dbReference type="HOGENOM" id="CLU_2168943_0_0_4"/>
    <dbReference type="OrthoDB" id="2064310at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid pMOL30"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF12938.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="110" mass="12299" checksum="38E6778FEF78C9E3" modified="2006-05-30" version="1">MKQFTFEDVLSLTFDELGAIEDPMQLAATAQVSPMLVRYVIRTDQLEERYRGVRMRTLLGAIDVAAAAVKWPNVVGQKALLAQKDADVDAYLDELQPHVAKAIELAPKYH</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LAV3</accession>
    <name>Q1LAV3_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Cyanate hydratase</fullName>
            <shortName evidence="3">Cyanase</shortName>
            <ecNumber evidence="3">4.2.1.104</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">Cyanate hydrolase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="3">Cyanate lyase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="3 5" type="primary">cynS</name>
        <name evidence="5" type="ordered locus">Rmet_5864</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="6">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="6">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 3">Catalyzes the reaction of cyanate with bicarbonate to produce ammonia and carbon dioxide.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>cyanate + 3 H(+) + hydrogencarbonate = 2 CO2 + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:11120"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:29195"/>
            <dbReference type="EC" id="4.2.1.104"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the cyanase family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.104" evidence="3"/>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12723.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5864"/>
    <dbReference type="EnsemblBacteria" id="ABF12723">
        <property type="protein sequence ID" value="ABF12723"/>
        <property type="gene ID" value="Rmet_5864"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5864"/>
    <dbReference type="eggNOG" id="COG1513">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_103452_1_0_4"/>
    <dbReference type="OMA" id="FLPYKAW"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008824">
        <property type="term" value="F:cyanate hydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009439">
        <property type="term" value="P:cyanate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00559">
        <property type="entry name" value="Cyanase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1160.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00535">
        <property type="entry name" value="Cyanate_hydrat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008076">
        <property type="entry name" value="Cyanase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003712">
        <property type="entry name" value="Cyanate_lyase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036581">
        <property type="entry name" value="Cyanate_lyase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010982">
        <property type="entry name" value="Lambda_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34186">
        <property type="entry name" value="PTHR34186"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02560">
        <property type="entry name" value="Cyanate_lyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001263">
        <property type="entry name" value="Cyanate_hydratas"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01693">
        <property type="entry name" value="CYANASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM01116">
        <property type="entry name" value="Cyanate_lyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47413">
        <property type="entry name" value="SSF47413"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55234">
        <property type="entry name" value="SSF55234"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00673">
        <property type="entry name" value="cynS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 3 5" id="KW-0456">Lyase</keyword>
    <keyword evidence="5" id="KW-0614">Plasmid</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cyanate_lyase" evidence="4">
        <location>
            <begin position="61"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="active site" evidence="3">
        <location>
            <position position="74"/>
        </location>
    </feature>
    <feature type="active site" evidence="3">
        <location>
            <position position="77"/>
        </location>
    </feature>
    <feature type="active site" evidence="3">
        <location>
            <position position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM01116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABF12723.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="133" mass="15083" checksum="D4B9D32646A0969F" modified="2006-05-30" version="1">MLRGIRWADVAERVGKSKEWTTAACLGQMAFDEAGARAVMEIFGLPAEAEPWLRAVPYKGSLPTQVPTDPLIYRFYELISVYGTTFKALIHEEFGDGIMSAIDFRMDLQREPDPNGDRVRIEMSGKFLPYKTY</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="68" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LB81</accession>
    <name>Q1LB81_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Capsule polysaccharide modification protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">kpsC</name>
        <name evidence="1" type="ordered locus">Rmet_5736</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
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            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid megaplasmid CH34</plasmid>
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        <property type="taxonomic scope" value="Bacteria"/>
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    <dbReference type="Proteomes" id="UP000002429">
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        <property type="evidence" value="ECO:0007669"/>
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    </dbReference>
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        <property type="entry name" value="Capsule_synth"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF12595.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="682" mass="75399" checksum="8A128B12826CB8B0" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="64" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LBB5</accession>
    <name>Q1LBB5_CUPMC</name>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name evidence="2" type="ordered locus">Rmet_5702</name>
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        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
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            <taxon>Betaproteobacteria</taxon>
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    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
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                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
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    </reference>
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    <dbReference type="EnsemblBacteria" id="ABF12561">
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    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5702"/>
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    <dbReference type="Proteomes" id="UP000002429">
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            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193264" evidence="1">
        <location>
            <begin position="21"/>
            <end position="384"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF12561.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="384" mass="41523" checksum="D8A4E8F61D4990C3" modified="2006-05-30" version="1">MLVTRLTLCVLALIATVAQAADKVAFAGFAYAGEARQIDSRFPLTRKVEAELAANGRSPSKLITQSLAANPPANFAITTDGMASLKDSDQALATALVVTSESVSSERFGSIYKVMTNLRGQVLVFDFKAMTVVRAYPLNVSYLDVLDHMPGDAEKRDRVKRLLAGGERPGLLDRYVRALSLAQLPSSGTRYLQIERVELSPGARAQLPEALRGDGVAEGWVADMFGEALLDKAQVPMLPFTKGYAIGNAMSMRFSDGEVFNLQLPKPDYTIDLSVKDFKRVEYGSSAAGTSYIYASYSHVSMAEPMSGRRYLDADFKNGEVKAVPVTQSRTDDFPAFAESLRGLFTKLADVVGGKDSDWLAAAASGDNVKSQIESTRGVIKSCR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="98" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LBK8</accession>
    <name>Q1LBK8_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Cytochrome o ubiquinol oxidase subunit I</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">cyoB</name>
        <name evidence="8" type="ordered locus">Rmet_5609</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="9">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="9">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the heme-copper respiratory oxidase family.</text>
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    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12468.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5609"/>
    <dbReference type="EnsemblBacteria" id="ABF12468">
        <property type="protein sequence ID" value="ABF12468"/>
        <property type="gene ID" value="Rmet_5609"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5609"/>
    <dbReference type="eggNOG" id="COG0843">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011899_7_1_4"/>
    <dbReference type="OMA" id="FRLWGPL"/>
    <dbReference type="OrthoDB" id="316745at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
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        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070469">
        <property type="term" value="C:respirasome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004129">
        <property type="term" value="F:cytochrome-c oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016682">
        <property type="term" value="F:oxidoreductase activity, acting on diphenols and related substances as donors, oxygen as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009060">
        <property type="term" value="P:aerobic respiration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Cyt_c_oxase-like_su1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036927">
        <property type="entry name" value="Cyt_c_oxase-like_su1_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000883">
        <property type="entry name" value="Cyt_C_Oxase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023615">
        <property type="entry name" value="Cyt_c_Oxase_su1_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014207">
        <property type="entry name" value="Cyt_c_ubiqinol_oxidase_su1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10422">
        <property type="entry name" value="PTHR10422"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00115">
        <property type="entry name" value="COX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01165">
        <property type="entry name" value="CYCOXIDASEI"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81442">
        <property type="entry name" value="SSF81442"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02843">
        <property type="entry name" value="CyoB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50855">
        <property type="entry name" value="COX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00077">
        <property type="entry name" value="COX1_CUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0249">Electron transport</keyword>
    <keyword evidence="5" id="KW-0349">Heme</keyword>
    <keyword evidence="5" id="KW-0408">Iron</keyword>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0614">Plasmid</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0679">Respiratory chain</keyword>
    <keyword evidence="2 5 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="26"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
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            <end position="92"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
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            <end position="136"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
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            <end position="180"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
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            <end position="223"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
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            <end position="265"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
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            <end position="308"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
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            <end position="342"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
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        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
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        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
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            <end position="450"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
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            <end position="483"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
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            <end position="528"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
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            <end position="617"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
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            <end position="641"/>
        </location>
    </feature>
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        <location>
            <begin position="49"/>
            <end position="569"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU000370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50855"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ABF12468.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="667" mass="74719" checksum="0AD299B2723DF470" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-10-07" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LBQ2</accession>
    <name>Q1LBQ2_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_5565</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
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        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
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        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12424.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF12424">
        <property type="protein sequence ID" value="ABF12424"/>
        <property type="gene ID" value="Rmet_5565"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5565"/>
    <dbReference type="HOGENOM" id="CLU_2318120_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="67"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF12424.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="99" mass="10895" checksum="BB7084D098122B33" modified="2006-05-30" version="1">MERLEARRKNWFEISREFQIRGNTLNSEVFSGRAPSAAFGLQGRPRSVCHDRHRLYASEVESEGRDARAVARSGVTSDTAEDDGGSDVGADGNTHCIGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LBU8</accession>
    <name>Q1LBU8_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Electron transfer flavoprotein beta-subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">etfB</name>
        <name evidence="4" type="ordered locus">Rmet_5519</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="5">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="5">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ETF beta-subunit/FixA family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12378.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519922.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5519"/>
    <dbReference type="EnsemblBacteria" id="ABF12378">
        <property type="protein sequence ID" value="ABF12378"/>
        <property type="gene ID" value="Rmet_5519"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5519"/>
    <dbReference type="eggNOG" id="COG2086">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_060196_0_0_4"/>
    <dbReference type="OMA" id="LEYSCAT"/>
    <dbReference type="OrthoDB" id="1027643at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01714">
        <property type="entry name" value="ETF_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000049">
        <property type="entry name" value="ET-Flavoprotein_bsu_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014730">
        <property type="entry name" value="ETF_a/b_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012255">
        <property type="entry name" value="ETF_b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033948">
        <property type="entry name" value="ETF_beta_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21294">
        <property type="entry name" value="PTHR21294"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01012">
        <property type="entry name" value="ETF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000090">
        <property type="entry name" value="Beta-ETF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00893">
        <property type="entry name" value="ETF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01065">
        <property type="entry name" value="ETF_BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0249">Electron transport</keyword>
    <keyword evidence="4" id="KW-0614">Plasmid</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ETF" evidence="3">
        <location>
            <begin position="23"/>
            <end position="211"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF12378.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="249" mass="26350" checksum="C514D05B3D650218" modified="2006-05-30" version="1">MKILVAVKRVVDSNVKVRVKSDGTGVDLANVKMSVNPFDEIAVEEAVRLKEAGVVTEVVAVSCGGAQCQETLRTAMAIGADRGILVESNEDLQPLAVAKLLKALIDKEQPGLVILGKQAIDDDSNQTGQMVAALAGLPQATFASKVVLADGKASVTREVDGGLETVSVKLPAVVTTDLRLNEPRYVTLPNIMKAKKKPLDTVKPEDLGVDVKPRLSTVKVIEPAKRSAGVMVPDVATLVQKLKTEAKVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LC91</accession>
    <name>Q1LC91_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Hemin-binding periplasmic protein hmuT</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">hmuT</name>
        <name evidence="3" type="ordered locus">Rmet_5376</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12235.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519782.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5376"/>
    <dbReference type="EnsemblBacteria" id="ABF12235">
        <property type="protein sequence ID" value="ABF12235"/>
        <property type="gene ID" value="Rmet_5376"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5376"/>
    <dbReference type="eggNOG" id="COG4558">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038034_6_0_4"/>
    <dbReference type="OMA" id="PNVGYMR"/>
    <dbReference type="OrthoDB" id="1473468at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002491">
        <property type="entry name" value="ABC_transptr_periplasmic_BD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01497">
        <property type="entry name" value="Peripla_BP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50983">
        <property type="entry name" value="FE_B12_PBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193313" evidence="1">
        <location>
            <begin position="25"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="domain" description="Fe/B12 periplasmic-binding" evidence="2">
        <location>
            <begin position="29"/>
            <end position="279"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF12235.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="283" mass="29321" checksum="69C8F03CC13E7F5F" modified="2006-05-30" version="1">MKILQSALITALMPLLCAAGVAHAAPPARVVGLGGPATEIVYALDAGKSMVGVDSSSIYPPAALKLPKVGYYRAFSVEGVVSLKPDLVLALDHAGPPQALEQLRKLGSKVVVVPSEPTLAALDQRILGVAAALDVPKQGQALVDKLHTELRDIKPSAEGLRVLMVSSHTGRMQAVGENTAGDAMLKLLGATNALAGQRGFKPFSAEAAAALRPDVIVTTSMSIDAVGSVDAFLAQPGLSATPAARDKRIVVMDDLLMLSIGPRLPEALRLLRNGLASRQVAAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="55" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LCB5</accession>
    <name>Q1LCB5_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_5352</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12211.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519757.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5352"/>
    <dbReference type="EnsemblBacteria" id="ABF12211">
        <property type="protein sequence ID" value="ABF12211"/>
        <property type="gene ID" value="Rmet_5352"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5352"/>
    <dbReference type="HOGENOM" id="CLU_2344603_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193053" evidence="2">
        <location>
            <begin position="20"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="22"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="23"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="40"/>
            <end position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF12211.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="97" mass="9618" checksum="8DE301F9E5B4BE5E" modified="2006-05-30" version="1">MKKLFAALASVAFVGTVFAQTGAPAAASATPSKSEPAQAQASAHKDKADVHTGAHKSTAKGTHHKAKATTDMAKSTDTTGATAGAAKTAAPDNSKKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-04-07" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LCF0</accession>
    <name>Q1LCF0_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Two-component response regulator (Receiver domain protein CheY-like)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_5317</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12176.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5317"/>
    <dbReference type="EnsemblBacteria" id="ABF12176">
        <property type="protein sequence ID" value="ABF12176"/>
        <property type="gene ID" value="Rmet_5317"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5317"/>
    <dbReference type="eggNOG" id="COG4566">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_69_8_4"/>
    <dbReference type="OMA" id="GPDLPRK"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Response regulatory" evidence="2">
        <location>
            <begin position="1"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="1">
        <location>
            <position position="36"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF12176.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="110" mass="11603" checksum="CBFEEDAE4EB85891" modified="2006-05-30" version="1">MGQLVRSFDLAVELYASGQALLQSASIGRIRCVVCDVRMPGIDGFALCTALRALGLDVPVILMTAYAQDGDEQRALDVGATGFLGKPFQERELLRCLARALASGESGSGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="72" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LCH4</accession>
    <name>Q1LCH4_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_5293</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12152.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519697.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5293"/>
    <dbReference type="ESTHER" id="ralme-q1lch4">
        <property type="family name" value="Hydrolase_RBBP9_YdeN"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF12152">
        <property type="protein sequence ID" value="ABF12152"/>
        <property type="gene ID" value="Rmet_5293"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5293"/>
    <dbReference type="eggNOG" id="COG3545">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_088863_2_2_4"/>
    <dbReference type="OMA" id="GWQGSPD"/>
    <dbReference type="OrthoDB" id="721431at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010662">
        <property type="entry name" value="Hydrolase_RBBP9/YdeN"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06821">
        <property type="entry name" value="Ser_hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="SSF53474"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF12152.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="189" mass="20834" checksum="910020ADBBE0816B" modified="2006-05-30" version="1">MTSGNQPTVVIVPGFRDHMPEHWQSLLAERLQQQGRAVRIVPQIDHDKRLRRARVENLERVVSGIPGPVILVAHSVGCLITVHWAQETKCAVQGALLAAPADFDNPLPPGYTDLPTLAKEGWTPIPRRQLKFPSIVVASRNDPLGKFERIQELAQAWGSRCIDAGEVGHLGPVDGFGTWPLAEELVQQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="108" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LCI3</accession>
    <name>Q1LCI3_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="10">ATP-dependent RNA helicase, specific for 23S rRNA</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">dbpA</name>
        <name evidence="10" type="ordered locus">Rmet_5284</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="11">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="11">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="6">Belongs to the DEAD box helicase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12143.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519689.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5284"/>
    <dbReference type="EnsemblBacteria" id="ABF12143">
        <property type="protein sequence ID" value="ABF12143"/>
        <property type="gene ID" value="Rmet_5284"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5284"/>
    <dbReference type="eggNOG" id="COG0513">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003041_1_3_4"/>
    <dbReference type="OMA" id="FGCQALV"/>
    <dbReference type="OrthoDB" id="626183at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005580">
        <property type="entry name" value="DbpA/CsdA_RNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000629">
        <property type="entry name" value="RNA-helicase_DEAD-box_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014014">
        <property type="entry name" value="RNA_helicase_DEAD_Q_motif"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03880">
        <property type="entry name" value="DbpA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00039">
        <property type="entry name" value="DEAD_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51195">
        <property type="entry name" value="Q_MOTIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 6 10" id="KW-0347">Helicase</keyword>
    <keyword evidence="2 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-0614">Plasmid</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Q_MOTIF" evidence="9">
        <location>
            <begin position="7"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="7">
        <location>
            <begin position="38"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="8">
        <location>
            <begin position="219"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Q motif" evidence="5">
        <location>
            <begin position="7"/>
            <end position="35"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ABF12143.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="467" mass="50753" checksum="76F7F6393DF7B4AE" modified="2006-05-30" version="1">MTSSAPTSFATLPLSAAMQATLVQLGYDAMTPIQAASLPLALAGHDLIAQARTGSGKTAAFALSLLHRLDPRPLDVQALVLCPTRELADQVTQEIRRLARAEDNVKVLTLSGGTPIRPQVESLVHGAHVVVGTPGRIIDHLDRGSLNIDAINTLVLDEADRMLDMGFHDDIAFIASHAPKDRQTLLFSATYPAAIDKLAHRFLRQPKTVKVEEAHDAATITQRFYEVEEGDRLNAVGRLLDHFRPATTLAFCNTKARCRDLADLLRAQGYAALELHGDLDQRDRDQVLVQFANRSCSVLVATDVAARGLDIAQLEAVINVDVTPDPEVHVHRVGRTGRAGEAGSAFSLVSLDEMGRVGNIEQHQGGEFEWHALDELKPSGGGRLLPPMVTLQMLGGRKEKIRPGDILGALTGEAGFTKEQIGKISVMEMSTYIAVDRAIGREAVKRLNEGKVKGRKVRVRMLTTDQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LCL2</accession>
    <name>Q1LCL2_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Flagellar secretion chaperone FliS</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">fliS</name>
        <name evidence="7" type="ordered locus">Rmet_5255</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="8">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="8">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Cytoplasm</location>
            <location evidence="1 6">Cytosol</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the FliS family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12114.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519660.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5255"/>
    <dbReference type="EnsemblBacteria" id="ABF12114">
        <property type="protein sequence ID" value="ABF12114"/>
        <property type="gene ID" value="Rmet_5255"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5255"/>
    <dbReference type="eggNOG" id="COG1516">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_080373_1_0_4"/>
    <dbReference type="OMA" id="EFRDTWK"/>
    <dbReference type="OrthoDB" id="1730695at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044780">
        <property type="term" value="P:bacterial-type flagellum assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd16098">
        <property type="entry name" value="FliS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003713">
        <property type="entry name" value="FliS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036584">
        <property type="entry name" value="FliS_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34773">
        <property type="entry name" value="PTHR34773"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02561">
        <property type="entry name" value="FliS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF039090">
        <property type="entry name" value="Flis"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101116">
        <property type="entry name" value="SSF101116"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00208">
        <property type="entry name" value="fliS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-1005">Bacterial flagellum biogenesis</keyword>
    <keyword evidence="7" id="KW-0966">Cell projection</keyword>
    <keyword evidence="5" id="KW-0143">Chaperone</keyword>
    <keyword evidence="7" id="KW-0969">Cilium</keyword>
    <keyword evidence="3 6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0282">Flagellum</keyword>
    <keyword evidence="7" id="KW-0614">Plasmid</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022795"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR039090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABF12114.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="144" mass="15132" checksum="8B6BC3CE6F0169F8" modified="2006-05-30" version="1">MFARRNAVNAYAQVGVETGAMSASPHQLIVMLYDGARAAIARAKFHLAAGDIAAKGNAISKSIDIIDNGLRAVLDHNAGGDVAADLERLYDYMSRQLMLANLRNDEALLDQVDSLLQDLSSAWAQIGNPAANGALAAQPIAANS</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LCM1</accession>
    <name>Q1LCM1_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_5246</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12105.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519651.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5246"/>
    <dbReference type="EnsemblBacteria" id="ABF12105">
        <property type="protein sequence ID" value="ABF12105"/>
        <property type="gene ID" value="Rmet_5246"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5246"/>
    <dbReference type="eggNOG" id="COG3238">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_068878_1_1_4"/>
    <dbReference type="OMA" id="ALQGPWW"/>
    <dbReference type="OrthoDB" id="2005506at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006750">
        <property type="entry name" value="YdcZ"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34821">
        <property type="entry name" value="PTHR34821"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04657">
        <property type="entry name" value="DMT_YdcZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="44"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="80"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="107"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="137"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="165"/>
            <end position="184"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF12105.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="184" mass="18918" checksum="42BF234EFF843890" modified="2006-05-30" version="1">MTSPLAASAGSLWMPLGVALLAGAMIPFQAGSNATLGRALGHPLWATVISLVISLVAVVPVLLALRAPPPNLAAAAQGPWWQWIGGLFGVIYITAALWLAPRMGAANFIVGAVAGQLLASMLVDHFGLVGLPARPVTLARLAGVVLVLAGICVMQWQPTRATGSALSERNLRGQSDQPRHPGSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LCP2</accession>
    <name>Q1LCP2_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Rmet_5225</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="5">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="5">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12084.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5225"/>
    <dbReference type="EnsemblBacteria" id="ABF12084">
        <property type="protein sequence ID" value="ABF12084"/>
        <property type="gene ID" value="Rmet_5225"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5225"/>
    <dbReference type="eggNOG" id="COG5361">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_057994_1_0_4"/>
    <dbReference type="OMA" id="QDVIRMN"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.600">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1610">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010621">
        <property type="entry name" value="DUF1214"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037049">
        <property type="entry name" value="DUF1214_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010679">
        <property type="entry name" value="DUF1254"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037050">
        <property type="entry name" value="DUF1254_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06742">
        <property type="entry name" value="DUF1214"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06863">
        <property type="entry name" value="DUF1254"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0614">Plasmid</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193346" evidence="1">
        <location>
            <begin position="24"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="domain" description="DUF1254" evidence="3">
        <location>
            <begin position="62"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="domain" description="DUF1214" evidence="2">
        <location>
            <begin position="247"/>
            <end position="331"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF06742"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF06863"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF12084.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="348" mass="37825" checksum="302BC162556C1EC4" modified="2006-05-30" version="1">MKRTMISNALMGFALVGVSTTYGQIAAAQATGAPVPVTVDNFVRAESDMYIVGVAKQGGVGKLQHRREPASIDDQAVIRLNRDTLYSSGVFDLDAGPVTITMPDPGKRFMSLQVISEDHYVPNVFYGAGTHTLTRENVGTRYVAAAIRTLVNPNDSNDLKQVHALQDAIKVSQKATGKLDLPNWDHASQKKVRDALLALSATTPDFKRSFGKKGEVDPVRHLMGTAAGWGGNPDKDAIYLNITPEKNDGKTVYRVNVKDVPVDGFWSISVYNAEGYFQRNAANAYTVNNITATKNSDGSIPIQFGGCNGQLPNCLPIVPGWNYTVRLYRPHAEIVRGAWSFPQAQAVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LCP5</accession>
    <name>Q1LCP5_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Hippurate hydrolase (Hippuricase) (Benzoylglycine amidohydrolase)</fullName>
            <ecNumber evidence="3">3.5.1.32</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">hipO</name>
        <name evidence="3" type="ordered locus">Rmet_5222</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EC" id="3.5.1.32" evidence="3"/>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12081.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519627.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5222"/>
    <dbReference type="EnsemblBacteria" id="ABF12081">
        <property type="protein sequence ID" value="ABF12081"/>
        <property type="gene ID" value="Rmet_5222"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5222"/>
    <dbReference type="eggNOG" id="COG1473">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023257_1_1_4"/>
    <dbReference type="OMA" id="HHEAEFD"/>
    <dbReference type="OrthoDB" id="796259at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047980">
        <property type="term" value="F:hippurate hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017439">
        <property type="entry name" value="Amidohydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036264">
        <property type="entry name" value="Bact_exopeptidase_dim_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002933">
        <property type="entry name" value="Peptidase_M20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011650">
        <property type="entry name" value="Peptidase_M20_dimer"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11014">
        <property type="entry name" value="PTHR11014"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07687">
        <property type="entry name" value="M20_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01546">
        <property type="entry name" value="Peptidase_M20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005962">
        <property type="entry name" value="Pept_M20D_amidohydro"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55031">
        <property type="entry name" value="SSF55031"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01891">
        <property type="entry name" value="amidohydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="M20_dimer" evidence="2">
        <location>
            <begin position="199"/>
            <end position="284"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF12081.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="398" mass="42703" checksum="3712425817D25694" modified="2006-05-30" version="1">MTMAIDSKASTALPDLLPLHDELAALRQHIHRHPELAFEEHDTADLVAGKLQEWGYHVTRGIGKTGLVGQLRVGNGDRCLGLRADMDALPIQEDTGLPYASIRPGLMHACGHDGHTAMLLGAAKHLAATRNFSGTLNLIFQPAEERGFDSGAKAMVADGLFDRFPCDMVFAMHNHPGAPQGRFLMRPGPFLAAGDRVFIKVIGLGGHAARPHLAVDPLVAAAAIVMALQTVVSRNVPPNESAVLTVGRLRAGDALNVIPAEAEIGISVRSFSPEVRALLRDRITTLVHMTAESYGAKAEIRYVEGYPVVDNAADAVALAAEVATALVGPEDVDRDYPRMMGSEDFAYMLQECPGALVRIGNGPSDGGRGLHNPRYDFNDRNLALGAAYWCRLTETFLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LCT0</accession>
    <name>Q1LCT0_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_5187</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12046.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5187"/>
    <dbReference type="EnsemblBacteria" id="ABF12046">
        <property type="protein sequence ID" value="ABF12046"/>
        <property type="gene ID" value="Rmet_5187"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5187"/>
    <dbReference type="eggNOG" id="COG2911">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG3055">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_414864_0_0_4"/>
    <dbReference type="OMA" id="TTTELRM"/>
    <dbReference type="OrthoDB" id="1977684at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034976">
        <property type="term" value="P:response to endoplasmic reticulum stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.80">
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015919">
        <property type="entry name" value="Cadherin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011043">
        <property type="entry name" value="Gal_Oxase/kelch_b-propeller"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037293">
        <property type="entry name" value="Gal_Oxidase_central_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006652">
        <property type="entry name" value="Kelch_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044832">
        <property type="entry name" value="NRP-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46034">
        <property type="entry name" value="PTHR46034"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01344">
        <property type="entry name" value="Kelch_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00612">
        <property type="entry name" value="Kelch"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49313">
        <property type="entry name" value="SSF49313"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50965">
        <property type="entry name" value="SSF50965"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193068" evidence="2">
        <location>
            <begin position="20"/>
            <end position="646"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF12046.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="646" mass="65375" checksum="F689AA3FEA4691B2" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LD08</accession>
    <name>Q1LD08_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">L-carnitine dehydratase/bile acid-inducible protein F</fullName>
            <ecNumber evidence="1">2.8.3.16</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_5109</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EC" id="2.8.3.16" evidence="1"/>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11968.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519515.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5109"/>
    <dbReference type="EnsemblBacteria" id="ABF11968">
        <property type="protein sequence ID" value="ABF11968"/>
        <property type="gene ID" value="Rmet_5109"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5109"/>
    <dbReference type="eggNOG" id="COG1804">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033975_0_0_4"/>
    <dbReference type="OMA" id="NCAPYEV"/>
    <dbReference type="OrthoDB" id="969868at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033608">
        <property type="term" value="F:formyl-CoA transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1540.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10540">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003673">
        <property type="entry name" value="CoA-Trfase_fam_III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044855">
        <property type="entry name" value="CoA-Trfase_III_dom3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023606">
        <property type="entry name" value="CoA-Trfase_III_dom_1_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02515">
        <property type="entry name" value="CoA_transf_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89796">
        <property type="entry name" value="SSF89796"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF11968.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="394" mass="42473" checksum="92B2D779360771EA" modified="2006-05-30" version="1">MLHRALEGIRVLDLSRILAGPWCTQNLADLGAEVVKVEHPQRGDDTRGWGPPYLDAADGSDARLSAYFISCNRGKQSVCIDYGQPEGVAQLLELARHADVLVENYKVGTLKRYGLDYASLKAINPRLIYLSITGFGQDGPMADKPGYDYVFQGMGGLMSYTGQPDGSPGAGPLRTGVAVVDVSTGMYATSAVLAALFQRQQTGEGQHLDIALLDVAVALNANQGANYLVSGKNPQRTGNAHPNCAPYEVFRCADGYLILAIGNDTQFARFCDVAGRPDLAQDPRYVTNSGRIEHLTELRKVLTALFPTRSRADWTSAFDAAGVPWGPIHTMEEVFAHPQVQHRQLRQVATHPVTGEVPMVRNPMLPANPQPLKPPPLLGEHTGVTAVAAIAAAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LD82</accession>
    <name>Q1LD82_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Copper and silver tricomponent efflux membrane fusion protein CusB (CzcB-like)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">cusB</name>
        <name evidence="5" type="ordered locus">Rmet_5032</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="6">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="6">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11894.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519445.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5032"/>
    <dbReference type="EnsemblBacteria" id="ABF11894">
        <property type="protein sequence ID" value="ABF11894"/>
        <property type="gene ID" value="Rmet_5032"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5032"/>
    <dbReference type="eggNOG" id="COG0845">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG5569">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018816_13_1_4"/>
    <dbReference type="OMA" id="NEPLYWV"/>
    <dbReference type="OrthoDB" id="949250at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.320">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021647">
        <property type="entry name" value="CusF_Ec"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042230">
        <property type="entry name" value="CusF_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032317">
        <property type="entry name" value="HlyD_D23"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045800">
        <property type="entry name" value="HMBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006143">
        <property type="entry name" value="RND_pump_MFP"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11604">
        <property type="entry name" value="CusF_Ec"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16576">
        <property type="entry name" value="HlyD_D23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19335">
        <property type="entry name" value="HMBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01730">
        <property type="entry name" value="RND_mfp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0614">Plasmid</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HMBD" evidence="4">
        <location>
            <begin position="45"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="domain" description="HlyD_D23" evidence="3">
        <location>
            <begin position="112"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="402"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="402"/>
            <end position="429"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF16576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF19335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABF11894.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="505" mass="53688" checksum="5DCBC86904CE9433" modified="2006-05-30" version="1">MNARLTITATCLVLIGVAGYSGYHLGALHTMPATSATQGDRKILYWHDPMVPGQRFDKPGKSPFMDMPLVPVYADGDDAGNGVNKGGVSIDSRIAQNLGIRTAEVKSAHMGAVVEAPGNVAIDERSIRVIQARTTAFVQHVGVRATLDPVRQGQTLVTFYAPDWVAAQEEYLAVMRLPVSLQTDLASAAKARMLQSGMTQSQADAVIRTGKLQSAQSIASPLDGLVTEIAVREGMTISPGMTLFRLADLTQVWVIAEVPEAQARSIAPGQTVTVSATAAPDPVQGKVDAILPDVDPATRTIKVRIVLPNKQRRLVPGMFASVRFENGDRQDVLQIPTEAVIRTGQRSLVMVDAGQRGYVSTEIRTGREVDGMTEVISGLRAGQRVVTSGQFLIDSEASLRGTSERLETPVTASAPSAPSTPSTATQEHEATGRIEAVNADSLTISHSAVPSAQWGAMTMDFAAHDAAQLKGLKPGDRIRFRFHLAEDGTAMLSSVQPAGAEGGQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="109" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LDF1</accession>
    <name>Q1LDF1_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Histidine kinase</fullName>
            <ecNumber evidence="2">2.7.13.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">Rmet_4963</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="11">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="11">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.</text>
            <dbReference type="EC" id="2.7.13.3"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.7.13.3" evidence="2"/>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11825.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519385.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4963"/>
    <dbReference type="EnsemblBacteria" id="ABF11825">
        <property type="protein sequence ID" value="ABF11825"/>
        <property type="gene ID" value="Rmet_4963"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4963"/>
    <dbReference type="eggNOG" id="COG2205">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_15_0_4"/>
    <dbReference type="OMA" id="MNAWLVK"/>
    <dbReference type="OrthoDB" id="1755994at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00082">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.160">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003661">
        <property type="entry name" value="HisK_dim/P"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036097">
        <property type="entry name" value="HisK_dim/P_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036641">
        <property type="entry name" value="HPT_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004358">
        <property type="entry name" value="Sig_transdc_His_kin-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008207">
        <property type="entry name" value="Sig_transdc_His_kin_Hpt_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00512">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01627">
        <property type="entry name" value="Hpt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00344">
        <property type="entry name" value="BCTRLSENSOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00388">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47226">
        <property type="entry name" value="SSF47226"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47384">
        <property type="entry name" value="SSF47384"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="SSF55874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50894">
        <property type="entry name" value="HPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="10" id="KW-0418">Kinase</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="10" id="KW-0614">Plasmid</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="23"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="7">
        <location>
            <begin position="522"/>
            <end position="742"/>
        </location>
    </feature>
    <feature type="domain" description="Response regulatory" evidence="8">
        <location>
            <begin position="886"/>
            <end position="1000"/>
        </location>
    </feature>
    <feature type="domain" description="HPt" evidence="9">
        <location>
            <begin position="1010"/>
            <end position="1113"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="5">
        <location>
            <position position="935"/>
        </location>
    </feature>
    <feature type="modified residue" description="Phosphohistidine" evidence="4">
        <location>
            <position position="1049"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ABF11825.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="1122" mass="122762" checksum="B9286DD0A1DBD933" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="92" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LDI9</accession>
    <name>Q1LDI9_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">4-hydroxyphenylpyruvate dioxygenase, oxidoreductase protein</fullName>
            <ecNumber evidence="7">1.13.11.27</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Rmet_4925</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="8">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="8">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </cofactor>
        <text evidence="5">Binds 1 Fe cation per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the 4HPPD family.</text>
    </comment>
    <dbReference type="EC" id="1.13.11.27" evidence="7"/>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11787.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519347.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4925"/>
    <dbReference type="EnsemblBacteria" id="ABF11787">
        <property type="protein sequence ID" value="ABF11787"/>
        <property type="gene ID" value="Rmet_4925"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4925"/>
    <dbReference type="eggNOG" id="COG3185">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_034004_1_0_4"/>
    <dbReference type="OMA" id="DPFPVKG"/>
    <dbReference type="OrthoDB" id="402639at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003868">
        <property type="term" value="F:4-hydroxyphenylpyruvate dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009072">
        <property type="term" value="P:aromatic amino acid family metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07250">
        <property type="entry name" value="HPPD_C_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd08342">
        <property type="entry name" value="HPPD_N_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.180.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005956">
        <property type="entry name" value="4OHPhenylPyrv_dOase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041735">
        <property type="entry name" value="4OHPhenylPyrv_dOase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041736">
        <property type="entry name" value="4OHPhenylPyrv_dOase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029068">
        <property type="entry name" value="Glyas_Bleomycin-R_OHBP_Dase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004360">
        <property type="entry name" value="Glyas_Fos-R_dOase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037523">
        <property type="entry name" value="VOC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11959">
        <property type="entry name" value="PTHR11959"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00903">
        <property type="entry name" value="Glyoxalase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF009283">
        <property type="entry name" value="HPP_dOase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54593">
        <property type="entry name" value="SSF54593"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01263">
        <property type="entry name" value="4HPPD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51819">
        <property type="entry name" value="VOC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="4 5" id="KW-0408">Iron</keyword>
    <keyword evidence="2 5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-0614">Plasmid</keyword>
    <keyword evidence="7" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="VOC" evidence="6">
        <location>
            <begin position="13"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="domain" description="VOC" evidence="6">
        <location>
            <begin position="159"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="162"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="241"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="323"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005877"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR009283-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABF11787.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="357" mass="40090" checksum="8CB5AC54C8B0BCB9" modified="2006-05-30" version="1">MADLFENPMKLMGFEFVEFASPTPNVLEPLFEKMGFTLVARHRSKDVVLYRQGDINFIVNNEPHSPAAYFAAEHGPSACGMAFRVKDSHQAYNRALMLGAQPVEIATGPMELRLPAIKGIGGAPLYLIDRFEDGKSIYDIDFEFIEGVDRHPKGHGLKLIDHLTHNVYRGRMAYWASFYERLFNFREIRYFDIQGEYTGLTSKAMTAPDGKIRIPLNEESSKGAGQIEEFLMAFNGEGIQHIAFSVDNLIEVIDSLQMAGVELMTAPNDYYYQALDTRLPGHGQPVDQLKARGILLDGTTEGGKPRLLLQIFSKTVLGPVFFEYIQRSGDDGFGEGNFKALFESLERDQIERGTLKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="95" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LDP5</accession>
    <name>Q1LDP5_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Transcriptional regulator, LysR family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_4869</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the LysR transcriptional regulatory family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11731.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008641456.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4869"/>
    <dbReference type="EnsemblBacteria" id="ABF11731">
        <property type="protein sequence ID" value="ABF11731"/>
        <property type="gene ID" value="Rmet_4869"/>
    </dbReference>
    <dbReference type="GeneID" id="60823878"/>
    <dbReference type="KEGG" id="rme:Rmet_4869"/>
    <dbReference type="eggNOG" id="COG0583">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039613_16_3_4"/>
    <dbReference type="OMA" id="LYAHRRN"/>
    <dbReference type="OrthoDB" id="750230at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005119">
        <property type="entry name" value="LysR_subst-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000847">
        <property type="entry name" value="Tscrpt_reg_HTH_LysR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00126">
        <property type="entry name" value="HTH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03466">
        <property type="entry name" value="LysR_substrate"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50931">
        <property type="entry name" value="HTH_LYSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH lysR-type" evidence="2">
        <location>
            <begin position="1"/>
            <end position="59"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF11731.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="308" mass="34226" checksum="DFDC46E4E7C5C402" modified="2006-05-30" version="1">MDRLVSMQAFTRVVDVGSFARAAESLDLPKATLTRLIQNLETHLGVKLLHRTTRRISVTTDGAAYYERCVRILADVEEAEQSLTRHNNSPRGTLCVDTTSGLAQMVLLPHLHDFFKAYPDLKLELTINGKPIDLLKEGVDVVLRVGALDESMVARRIGQLKLAFYASPIYLRRVGTPQNLTELAQHKAVNFLSNRTGREMPWPLSRGGERTEVLLPSAISTNDADVYMACALEGHGIARISRAMAEPYVNSGRLVEVMSDWQTDELPISAMYPQNRHLSAKVRVFVNWAAELFSRHPHFGAAPQAVAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="105" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LDY0</accession>
    <name>Q1LDY0_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 8">S-(hydroxymethyl)glutathione dehydrogenase</fullName>
            <ecNumber evidence="5 8">1.1.1.284</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">frmA</name>
        <name evidence="10" type="ordered locus">Rmet_4784</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="11">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="11">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 8">
            <text>NAD(+) + S-(hydroxymethyl)glutathione = H(+) + NADH + S-formylglutathione</text>
            <dbReference type="Rhea" id="RHEA:19985"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57688"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:58758"/>
            <dbReference type="EC" id="1.1.1.284"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>NADP(+) + S-(hydroxymethyl)glutathione = H(+) + NADPH + S-formylglutathione</text>
            <dbReference type="Rhea" id="RHEA:19981"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57688"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:58758"/>
            <dbReference type="EC" id="1.1.1.284"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3 8">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="4 8">Belongs to the zinc-containing alcohol dehydrogenase family. Class-III subfamily.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.284" evidence="5 8"/>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11646.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519214.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4784"/>
    <dbReference type="EnsemblBacteria" id="ABF11646">
        <property type="protein sequence ID" value="ABF11646"/>
        <property type="gene ID" value="Rmet_4784"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4784"/>
    <dbReference type="eggNOG" id="COG1062">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026673_14_0_4"/>
    <dbReference type="OMA" id="IHHYMGT"/>
    <dbReference type="OrthoDB" id="1859760at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106322">
        <property type="term" value="F:S-(hydroxymethyl)glutathione dehydrogenase NAD activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106321">
        <property type="term" value="F:S-(hydroxymethyl)glutathione dehydrogenase NADP activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006069">
        <property type="term" value="P:ethanol oxidation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd08300">
        <property type="entry name" value="alcohol_DH_class_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013149">
        <property type="entry name" value="ADH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014183">
        <property type="entry name" value="ADH_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013154">
        <property type="entry name" value="ADH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002328">
        <property type="entry name" value="ADH_Zn_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020843">
        <property type="entry name" value="PKS_ER"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08240">
        <property type="entry name" value="ADH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00107">
        <property type="entry name" value="ADH_zinc_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00829">
        <property type="entry name" value="PKS_ER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="SSF50129"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02818">
        <property type="entry name" value="adh_III_F_hyde"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00059">
        <property type="entry name" value="ADH_ZINC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0520">NAD</keyword>
    <keyword evidence="7 8 10" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-0614">Plasmid</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="PKS_ER" evidence="9">
        <location>
            <begin position="12"/>
            <end position="366"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001646"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012309"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00021865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU362016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ABF11646.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="368" mass="39361" checksum="AFFF9776A9523992" modified="2006-05-30" version="1">MKTKAAIAWKAGAPLTIEEVDLDGPRAGEVLVEIKATGICHTDYYTLSGADPEGIFPAILGHEGAGIVTDVGPGVTSLKPGDHVIPLYTPECRQCKFCLSRKTNLCQAIRATQGKGLMPDGTSRFSIDGKPIFHYMGTSTFANHIVVPEIALAKIRPDAPFDKVCYIGCGVTTGVGAVIFTAKVEAGANVVVFGLGGIGLNVIQGAKMVGADKIIGVDLNPAREAMARKFGMTHFVNPKDVDNVVDHIVQLTDGGADYSFECIGNTQVMRQALECCHKGWGKSIIIGVAEAGAEISTRPFQLVTGREWKGSAFGGARGRTDVPKIVDWYMDGKLNIDDLITHTLPLDRINEGFDLMKRGESIRSVVLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="108" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LE49</accession>
    <name>Q1LE49_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Two component transcriptional regulator, LuxR family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Rmet_4715</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="7">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="7">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11577.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519148.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4715"/>
    <dbReference type="EnsemblBacteria" id="ABF11577">
        <property type="protein sequence ID" value="ABF11577"/>
        <property type="gene ID" value="Rmet_4715"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4715"/>
    <dbReference type="eggNOG" id="COG2197">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_90_1_4"/>
    <dbReference type="OMA" id="LDKYATH"/>
    <dbReference type="OrthoDB" id="1687028at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06170">
        <property type="entry name" value="LuxR_C_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016032">
        <property type="entry name" value="Sig_transdc_resp-reg_C-effctor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000792">
        <property type="entry name" value="Tscrpt_reg_LuxR_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00196">
        <property type="entry name" value="GerE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00038">
        <property type="entry name" value="HTHLUXR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00421">
        <property type="entry name" value="HTH_LUXR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46894">
        <property type="entry name" value="SSF46894"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00622">
        <property type="entry name" value="HTH_LUXR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50043">
        <property type="entry name" value="HTH_LUXR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="6" id="KW-0614">Plasmid</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="198"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="domain" description="Response regulatory" evidence="5">
        <location>
            <begin position="6"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="domain" description="HTH luxR-type" evidence="4">
        <location>
            <begin position="149"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="2">
        <location>
            <position position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABF11577.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="264" mass="28811" checksum="940A7888A86A6D73" modified="2006-05-30" version="1">MNNNCSVLIVEDHNLLRAGLRSMLSAQMGYHVAGEARDGREACQLAISLCPDLILMDLSLPGMNGIDAIAAIKRRSPGIRIIALSVHQNDEYVREALRAGVDGYVLKDTSFDDLVTAMRVVLLGKKHLSPELYGNLVESYVSGHEMSTAKTGWDLLTARERSVLKLVAEGRSNRQVGIYMNLSPKTIEKYRASLMRKLGITNLTELVLIAISMGLITSLARKYCDDNHPSVTNHDGLDAVPLPAVRRQMLDEGRAAGSDGEASP</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="106" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LED7</accession>
    <name>Q1LED7_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Two component transcriptional regulator, LuxR family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Rmet_4627</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="6">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="6">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11489.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519065.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4627"/>
    <dbReference type="EnsemblBacteria" id="ABF11489">
        <property type="protein sequence ID" value="ABF11489"/>
        <property type="gene ID" value="Rmet_4627"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4627"/>
    <dbReference type="eggNOG" id="COG2197">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_90_1_4"/>
    <dbReference type="OMA" id="VSGMTIK"/>
    <dbReference type="OrthoDB" id="1687028at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06170">
        <property type="entry name" value="LuxR_C_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016032">
        <property type="entry name" value="Sig_transdc_resp-reg_C-effctor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000792">
        <property type="entry name" value="Tscrpt_reg_LuxR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00196">
        <property type="entry name" value="GerE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00038">
        <property type="entry name" value="HTHLUXR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00421">
        <property type="entry name" value="HTH_LUXR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46894">
        <property type="entry name" value="SSF46894"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50043">
        <property type="entry name" value="HTH_LUXR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="5" id="KW-0614">Plasmid</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Response regulatory" evidence="4">
        <location>
            <begin position="7"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="domain" description="HTH luxR-type" evidence="3">
        <location>
            <begin position="149"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="2">
        <location>
            <position position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABF11489.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="216" mass="22652" checksum="4952D2A18C1A013D" modified="2006-05-30" version="1">MPPDTISVVIADDHPAIQLAVKSTLTEIPTVQIAAMCASGGELFAAVEAHRPDLIVTDFTMGRSEGNDDGLRLIQRLRQSGPDTPIVVFTMLTNGGLLTRIQESGVAGIVGKDEEPAILRQICMDAVSGNGLRLSPGISARMASAGAMAGGGISPLSPKEIEVVRMFAAGSPLTEIAGYLHRTLATVATQKRSAMRKLNITSNADLVTYARDNGLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LEE1</accession>
    <name>Q1LEE1_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_4623</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11485.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4623"/>
    <dbReference type="EnsemblBacteria" id="ABF11485">
        <property type="protein sequence ID" value="ABF11485"/>
        <property type="gene ID" value="Rmet_4623"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4623"/>
    <dbReference type="HOGENOM" id="CLU_194587_0_0_4"/>
    <dbReference type="OMA" id="PDIPHAK"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="38"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="50"/>
            <end position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF11485.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="82" mass="8569" checksum="58C9A79CEDD7B61C" modified="2006-05-30" version="1">MRGFQSTPDIPHAKDPMQRMRLIRALLVASLVAIGTVACGGNDDSPPASSPQQPVSPPQQPSTPDGAPNPTEPAKPDMRCAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="107" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LEG5</accession>
    <name>Q1LEG5_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Serine-based site-specific recombinase activity TnpR (Resolvase) Tn6050</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">tnpR</name>
        <name evidence="8" type="ordered locus">Rmet_4473</name>
        <name evidence="9" type="ordered locus">Rmet_4599</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="9">
        <name>megaplasmid</name>
    </geneLocation>
    <geneLocation type="plasmid" evidence="10">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="9" key="1">
        <citation type="submission" date="2006-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of the chromosome of Ralstonia metallidurans CH34.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Copeland A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Barry K."/>
                <person name="Detter J.C."/>
                <person name="Glavina del Rio T."/>
                <person name="Hammon N."/>
                <person name="Israni S."/>
                <person name="Dalin E."/>
                <person name="Tice H."/>
                <person name="Martinez M."/>
                <person name="Goltsman E."/>
                <person name="Pitluck S."/>
                <person name="Schmutz J."/>
                <person name="Larimer F."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Kim E."/>
                <person name="Mergeay M."/>
                <person name="Benotmane M.A."/>
                <person name="Vallaeys T."/>
                <person name="Michaux A."/>
                <person name="Monchy S."/>
                <person name="Dunn J."/>
                <person name="McCorkle S."/>
                <person name="Taghavi S."/>
                <person name="van der Lelie D."/>
                <person name="Richardson P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">CH34</strain>
            <plasmid evidence="9">megaplasmid</plasmid>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="10">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2010-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Benotmane R."/>
                <person name="Lapidus A."/>
                <person name="McCorkle S."/>
                <person name="Monchy S."/>
                <person name="Taghavi S."/>
                <person name="van der Lelie N."/>
                <person name="Dunn J."/>
                <person name="Leys N."/>
                <person name="Mergeay M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="9">CH34</strain>
            <plasmid evidence="9">megaplasmid</plasmid>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the site-specific recombinase resolvase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11338.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11461.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_003124096.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4473"/>
    <dbReference type="EnsemblBacteria" id="ABF11338">
        <property type="protein sequence ID" value="ABF11338"/>
        <property type="gene ID" value="Rmet_4473"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF11461">
        <property type="protein sequence ID" value="ABF11461"/>
        <property type="gene ID" value="Rmet_4599"/>
    </dbReference>
    <dbReference type="GeneID" id="34794304"/>
    <dbReference type="KEGG" id="rme:Rmet_4473"/>
    <dbReference type="KEGG" id="rme:Rmet_4599"/>
    <dbReference type="eggNOG" id="COG1961">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010686_8_3_4"/>
    <dbReference type="OMA" id="QRQMEGI"/>
    <dbReference type="OrthoDB" id="1791095at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000150">
        <property type="term" value="F:DNA strand exchange activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1390">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006118">
        <property type="entry name" value="Recombinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006119">
        <property type="entry name" value="Resolv_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036162">
        <property type="entry name" value="Resolvase-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006120">
        <property type="entry name" value="Resolvase_HTH_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02796">
        <property type="entry name" value="HTH_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00239">
        <property type="entry name" value="Resolvase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00857">
        <property type="entry name" value="Resolvase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53041">
        <property type="entry name" value="SSF53041"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00397">
        <property type="entry name" value="RECOMBINASES_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00398">
        <property type="entry name" value="RECOMBINASES_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51736">
        <property type="entry name" value="RECOMBINASES_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0229">DNA integration</keyword>
    <keyword evidence="4" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="9" id="KW-0614">Plasmid</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Resolvase/invertase-type recombinase catalytic" evidence="7">
        <location>
            <begin position="4"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="active site" description="O-(5'-phospho-DNA)-serine intermediate" evidence="5 6">
        <location>
            <position position="12"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR606118-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51736"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ABF11338.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ABF11461.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="186" mass="21596" checksum="63CE0709762CF8DE" modified="2006-05-30" version="1">MHGQRIGYVRVSSFDQNPERQLEQIQVDKVFTDKASGKDTRRPELERLLAFVREGDTVVVHSMDRLARNLDDLRRLVQGLTQRGVRIEFLKEHLTFTGEDSPMANLMLSVMGAFAEFERALIRERQREGIALAKQRGAYRGRKKSLSSERIAELRQRVEAGEQKTKLAREFGISRETLYQYLRTDQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="67" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LEQ3</accession>
    <name>Q1LEQ3_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">T3SS_YcgR_PilZN domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_4508</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11373.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518966.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4508"/>
    <dbReference type="EnsemblBacteria" id="ABF11373">
        <property type="protein sequence ID" value="ABF11373"/>
        <property type="gene ID" value="Rmet_4508"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4508"/>
    <dbReference type="eggNOG" id="COG5581">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_073573_0_0_4"/>
    <dbReference type="OrthoDB" id="1377603at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009926">
        <property type="entry name" value="T3SS_YcgR_PilZN"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12945">
        <property type="entry name" value="YcgR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="T3SS_YcgR_PilZN" evidence="1">
        <location>
            <begin position="76"/>
            <end position="155"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12945"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF11373.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="290" mass="31261" checksum="A9AF569E1B534B6F" modified="2006-05-30" version="1">MRREILANEIELGQPLKSALVDELGFPVADEGTVFQTGDEVALVLQAGRCFWADDMAPLRPDEPSPPWAGGYGLLVGTLLHVKRPGDLERAWVSRLIGFNDDAVFVAWPGLAGMSAEPCDGDLVLVRGFTGTSIHSFSARVRATSRTPFPYWMLTDISHVDAREMRGSIRVPVRLAGRMSRVDGAEAPQTVLITDLSTGGVSVSTANDALTAPGLQVRLAFTLRTGGIKSTFSLEGWTRQDPNAGAAAPYHSVGVAFDGYGEREALLLRSFVHEQLLESATMRPAKLAKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="64" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LF85</accession>
    <name>Q1LF85_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transmembrane protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_4326</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11191.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518815.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4326"/>
    <dbReference type="EnsemblBacteria" id="ABF11191">
        <property type="protein sequence ID" value="ABF11191"/>
        <property type="gene ID" value="Rmet_4326"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4326"/>
    <dbReference type="eggNOG" id="ENOG5032SXK">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_108389_0_0_4"/>
    <dbReference type="OMA" id="DRLCANY"/>
    <dbReference type="OrthoDB" id="1767215at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="32"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="65"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="121"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="161"/>
            <end position="179"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF11191.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="191" mass="20717" checksum="06C0A537F096F314" modified="2006-05-30" version="1">MSRKSWHFVLLILLIPALPPLRDVVEASMSSLMLCLLPCLAAAGWAFAELYVISRRSLMDAMRPYGLALCTLCTFSYGVWMVPIAIDLSRMSMATGIARDASVCVAGFSMALAIRISPWPVLLFFIGNLVWMGVTVGLIFVEEPVRLCANYLLGDQHQAGAGIVAYSAGAGLWLLGWVFRKAGLADLSSDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LF86</accession>
    <name>Q1LF86_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein SCO1/SenC/PrrC,involved in biogenesis of respiratory and photosynthetic systems</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_4325</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SCO1/2 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11190.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4325"/>
    <dbReference type="EnsemblBacteria" id="ABF11190">
        <property type="protein sequence ID" value="ABF11190"/>
        <property type="gene ID" value="Rmet_4325"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4325"/>
    <dbReference type="eggNOG" id="COG1999">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_050131_4_4_4"/>
    <dbReference type="OMA" id="DFIYTRC"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="CDD" id="cd02968">
        <property type="entry name" value="SCO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003782">
        <property type="entry name" value="SCO1/SenC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02630">
        <property type="entry name" value="SCO1-SenC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="SSF52833"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010996"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF11190.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="182" mass="19638" checksum="B99F9BCD24E3B922" modified="2006-05-30" version="1">MSAWTLDERRDLRIASLDLRMPATAVRNQQGHGVQLFSGNATSTVYIVDFIYTRCNTVCSALGGEFAQLSHQVKADGLSGQLGLVSISLDARDTAGDLKTYAATYRASLPEWEVVTASDSRELNALLRQADVIAIPDGMGGITHNGALDVVSANGVVLATYPMEQFQQAYLFARSVVRQAAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-10-07" version="72" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LFC5</accession>
    <name>Q1LFC5_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Glyoxalase/bleomycin resistance protein/dioxygenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_4285</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11151.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4285"/>
    <dbReference type="EnsemblBacteria" id="ABF11151">
        <property type="protein sequence ID" value="ABF11151"/>
        <property type="gene ID" value="Rmet_4285"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4285"/>
    <dbReference type="eggNOG" id="COG0346">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046006_13_3_4"/>
    <dbReference type="OMA" id="VMNHGWI"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051213">
        <property type="term" value="F:dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.180.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029068">
        <property type="entry name" value="Glyas_Bleomycin-R_OHBP_Dase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004360">
        <property type="entry name" value="Glyas_Fos-R_dOase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037523">
        <property type="entry name" value="VOC"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00903">
        <property type="entry name" value="Glyoxalase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54593">
        <property type="entry name" value="SSF54593"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51819">
        <property type="entry name" value="VOC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="VOC" evidence="1">
        <location>
            <begin position="23"/>
            <end position="136"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF11151.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="137" mass="15245" checksum="98D96AA069EA1AC4" modified="2006-05-30" version="1">MTCAPASGHWQPGLINQKECNLKVKRVVANIEVADVTRARAFYHDVLGLELLMDHGWIRTYGSQSEMTIQVSFATEGGSGAPVPDLSIEVDDADEAHRRMRDAGFSIEHGPVDEPWGVRRFFVRDPYGKLVNILAHT</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LFH5</accession>
    <name>Q1LFH5_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Putative D-3-phosphoglycerate dehydrogenase</fullName>
            <ecNumber evidence="5">1.1.1.95</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Rmet_4234</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="6">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="6">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.95" evidence="5"/>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11101.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518724.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4234"/>
    <dbReference type="EnsemblBacteria" id="ABF11101">
        <property type="protein sequence ID" value="ABF11101"/>
        <property type="gene ID" value="Rmet_4234"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4234"/>
    <dbReference type="eggNOG" id="COG0111">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019796_1_3_4"/>
    <dbReference type="OMA" id="QSDLHEA"/>
    <dbReference type="OrthoDB" id="1638924at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004617">
        <property type="term" value="F:phosphoglycerate dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006139">
        <property type="entry name" value="D-isomer_2_OHA_DH_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029753">
        <property type="entry name" value="D-isomer_DH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006140">
        <property type="entry name" value="D-isomer_DH_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00389">
        <property type="entry name" value="2-Hacid_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02826">
        <property type="entry name" value="2-Hacid_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00671">
        <property type="entry name" value="D_2_HYDROXYACID_DH_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 2 5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-0614">Plasmid</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="2-Hacid_dh" evidence="3">
        <location>
            <begin position="8"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="domain" description="2-Hacid_dh_C" evidence="4">
        <location>
            <begin position="111"/>
            <end position="283"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU003719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF02826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABF11101.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="312" mass="32629" checksum="329CC589BA073FD4" modified="2006-05-30" version="1">MKKQPVVLVTGNDLAPQALELLKDFEVRFAGKSCTEDVLLALAKETDPVAIIVRYGRIGASIMDAAPSLKVISKHGSGIDVIDQKAAAERGIAVKAAVGANAAAVAEHAWALILACAKSVPHLNDRMREGYWDKSIHKSIELNGRTLGIVGLGEIGRRVAVTGVVLGMRVIAFDPYANAAPDGVELVNDLHDIYRNADVVSLHCPLTESNRGMLHRETLGLFKNGAILINTARGGLVDEPALAEALTNGMLYAAGLDSFAVEPMTAPHPLQQIPNAILTPHIGGVSDAAYVSMGVGAATNVLAVLGSVTADG</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="96" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LFL8</accession>
    <name>Q1LFL8_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Transcriptional regulator, LysR family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_4191</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the LysR transcriptional regulatory family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11058.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518680.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4191"/>
    <dbReference type="EnsemblBacteria" id="ABF11058">
        <property type="protein sequence ID" value="ABF11058"/>
        <property type="gene ID" value="Rmet_4191"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4191"/>
    <dbReference type="eggNOG" id="COG0583">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039613_6_1_4"/>
    <dbReference type="OMA" id="HYEIDLG"/>
    <dbReference type="OrthoDB" id="750230at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005119">
        <property type="entry name" value="LysR_subst-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000847">
        <property type="entry name" value="Tscrpt_reg_HTH_LysR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017724">
        <property type="entry name" value="Tscrpt_reg_LysR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00126">
        <property type="entry name" value="HTH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03466">
        <property type="entry name" value="LysR_substrate"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00039">
        <property type="entry name" value="HTHLYSR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03339">
        <property type="entry name" value="phn_lysR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50931">
        <property type="entry name" value="HTH_LYSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH lysR-type" evidence="2">
        <location>
            <begin position="1"/>
            <end position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF11058.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="304" mass="33480" checksum="B7D2B9E1BB564B79" modified="2006-05-30" version="1">MLGDLLMTFYEVARQGSITTAARQLRVSQPTVTGRIRQLEELYGVELFHRRASRVDLSDTGVALMPLAEQIVQQESNADFLLRNAGNLRMGNLRIGATGPYYILRSVAAFRQRYPAIGVSIQIGNSRQMLDALFEYRIDAAVSSHAVNDDRLHRVRLAADPMVLVTHPAHPFARRPRVEVSELAACHLLVREQGSMTREATESALRAVGEELPAHTVIGSREAICEAIRHNLGASVMPMGEVPRDPALTVVPFASRAPVIHEYLYCLNSRRETRLLSALLDSLVLPGAASGRHCEVPPPAPILN</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LFM7</accession>
    <name>Q1LFM7_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Carboxylesterase 2 (Esterase II)</fullName>
            <ecNumber evidence="2">3.1.1.1</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">estB</name>
        <name evidence="2" type="ordered locus">Rmet_4182</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EC" id="3.1.1.1" evidence="2"/>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11049.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518670.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4182"/>
    <dbReference type="ESTHER" id="ralme-q1lfm7">
        <property type="family name" value="LYsophospholipase_carboxylesterase"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF11049">
        <property type="protein sequence ID" value="ABF11049"/>
        <property type="gene ID" value="Rmet_4182"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4182"/>
    <dbReference type="eggNOG" id="COG0400">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_049413_3_2_4"/>
    <dbReference type="OMA" id="WYDILAM"/>
    <dbReference type="OrthoDB" id="1195966at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080030">
        <property type="term" value="F:methyl indole-3-acetate esterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003140">
        <property type="entry name" value="PLipase/COase/thioEstase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02230">
        <property type="entry name" value="Abhydrolase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="SSF53474"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Abhydrolase_2" evidence="1">
        <location>
            <begin position="18"/>
            <end position="219"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02230"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF11049.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="223" mass="23868" checksum="AF0E263A221585B2" modified="2006-05-30" version="1">MSEPLPAIEIETAPNPAFSVIWMHGLGADGSDFVPVVPELGLPETLAVRFIFPNAPAIPVTCNGGYVMPAWYDIYSLDEAGRRADERGILQSRDAIRTLIARENARGVPTNRIVLAGFSQGGAIAYTTALTHPETLAGVIALSTYIPSTDLIASEAAPANAATPILAAHGTEDGVVPLMLGTRARDWVAAAGHPVTWLTYRMPHSVCIEEIVDIGQWLQERFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="69" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LFT3</accession>
    <name>Q1LFT3_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Putative nitrogen fixation protein NifQ</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Rmet_4126</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="5">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="5">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Probably involved in molybdenum processing.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NifQ family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10993.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518619.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4126"/>
    <dbReference type="EnsemblBacteria" id="ABF10993">
        <property type="protein sequence ID" value="ABF10993"/>
        <property type="gene ID" value="Rmet_4126"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4126"/>
    <dbReference type="eggNOG" id="ENOG50327FW">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_094545_2_0_4"/>
    <dbReference type="OMA" id="MVARRAM"/>
    <dbReference type="OrthoDB" id="1740725at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030151">
        <property type="term" value="F:molybdenum ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009399">
        <property type="term" value="P:nitrogen fixation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006975">
        <property type="entry name" value="NifQ"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04891">
        <property type="entry name" value="NifQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0535">Nitrogen fixation</keyword>
    <keyword evidence="4" id="KW-0614">Plasmid</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF10993.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="210" mass="22815" checksum="9212402FC15F1D96" modified="2006-05-30" version="1">MNRSLLLLDAFAAQPGDPVTRALAGVLESACAGRLPRFAQWLGLPPDAFRHMLDHCFPGAAQAGWAPDVPPQAPDSLPCEFADLVEMLEDGCTPGAAGTLVPLVHWAAHALACGCFGHTHLWQDMGLSGRDDVSALLRQVFWPVFAANTTDMKWKKFFYHRVCERLDIHPCPEPSCEGCDHYAACHGAESAIESAIESVIESVIVRQPRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="117" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LFU7</accession>
    <name>Q1LFU7_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Oxidoreductase/oxygenase, vanB family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Rmet_4112</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="7">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="7">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10979.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518607.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4112"/>
    <dbReference type="EnsemblBacteria" id="ABF10979">
        <property type="protein sequence ID" value="ABF10979"/>
        <property type="gene ID" value="Rmet_4112"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4112"/>
    <dbReference type="eggNOG" id="COG1018">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003827_17_0_4"/>
    <dbReference type="OMA" id="EWITPCV"/>
    <dbReference type="OrthoDB" id="1885467at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00207">
        <property type="entry name" value="fer2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036010">
        <property type="entry name" value="2Fe-2S_ferredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001041">
        <property type="entry name" value="2Fe-2S_ferredoxin-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006058">
        <property type="entry name" value="2Fe2S_fd_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012675">
        <property type="entry name" value="Beta-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017927">
        <property type="entry name" value="FAD-bd_FR_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039261">
        <property type="entry name" value="FNR_nucleotide-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000951">
        <property type="entry name" value="Ph_dOase_redase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017938">
        <property type="entry name" value="Riboflavin_synthase-like_b-brl"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00111">
        <property type="entry name" value="Fer2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00409">
        <property type="entry name" value="PHDIOXRDTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52343">
        <property type="entry name" value="SSF52343"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54292">
        <property type="entry name" value="SSF54292"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63380">
        <property type="entry name" value="SSF63380"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00197">
        <property type="entry name" value="2FE2S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51384">
        <property type="entry name" value="FAD_FR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0001">2Fe-2S</keyword>
    <keyword evidence="3" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0614">Plasmid</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD-binding FR-type" evidence="5">
        <location>
            <begin position="5"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABF10979.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="317" mass="33766" checksum="0C2CA0C021CC03F5" modified="2006-05-30" version="1">MAARPIKTVSVVVSAIDEPLPGVKRFVLSDQDHWPLPPFTPGAHIDLHLGGGLVRTYSLCNEPSDRNRYVIAVKHEVAGRGGSAFVHERLDVGASIGVSLPRGGIRTTDTGMNVFIAGGIGVTPFISTIRDLELRGQTNYVLHWSSAGVPSLVDMLGDAIAAGRVRLYDTRVESAPDMDAILNACGENAKAYCCGPMRMLDAFERIVEGWPDARKHIERFAPPKPVEDTDAAPYTVVLARSGKEAIVEPHVGLVGTLEALGADVSVSCGGGVCGACRTTWLEGPPIHRDRVLSPEERAQDVMVCVAGCAGSRLVLDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LG07</accession>
    <name>Q1LG07_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Putative glutathione S-transferase</fullName>
            <ecNumber evidence="4">2.5.1.18</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Rmet_4051</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="5">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="5">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the GST superfamily.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.18" evidence="4"/>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10919.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4051"/>
    <dbReference type="EnsemblBacteria" id="ABF10919">
        <property type="protein sequence ID" value="ABF10919"/>
        <property type="gene ID" value="Rmet_4051"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4051"/>
    <dbReference type="eggNOG" id="COG0625">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011226_6_0_4"/>
    <dbReference type="OMA" id="AYKVRLF"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004364">
        <property type="term" value="F:glutathione transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006749">
        <property type="term" value="P:glutathione metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010987">
        <property type="entry name" value="Glutathione-S-Trfase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036282">
        <property type="entry name" value="Glutathione-S-Trfase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040079">
        <property type="entry name" value="Glutathione_S-Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004045">
        <property type="entry name" value="Glutathione_S-Trfase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004046">
        <property type="entry name" value="GST_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00043">
        <property type="entry name" value="GST_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02798">
        <property type="entry name" value="GST_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00019">
        <property type="entry name" value="Glutathione_Transferase_(cytos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47616">
        <property type="entry name" value="SSF47616"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="SSF52833"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50405">
        <property type="entry name" value="GST_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50404">
        <property type="entry name" value="GST_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0614">Plasmid</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="GST N-terminal" evidence="2">
        <location>
            <begin position="19"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="domain" description="GST C-terminal" evidence="3">
        <location>
            <begin position="105"/>
            <end position="223"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU003494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF10919.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="223" mass="24366" checksum="E31ED7383D5F6B74" modified="2006-05-30" version="1">MMRARHLPSRRTIASKDVPTMKLYVHPLSGHAHRAQLLLSLLGQPAELIEVDLMTGAHKSPEFLKLNPFGQIPVLVDGETVVPDSNAILVYLSKKLGRTDWLPEAPATAAAVQRWLSVAAGDIAFGPARARIATLFNAPYDLDDAVARAHVVLKRMDDALADRPWIAADHPTIADVALYSYTARAPEGFVDLRDYGKVRQWLARVEALPGFVPIVKSPVGLEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LG59</accession>
    <name>Q1LG59_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Putative TonB-dependent siderophore receptor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">Rmet_3999</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="11">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="11">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cell outer membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the TonB-dependent receptor family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10867.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518506.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3999"/>
    <dbReference type="EnsemblBacteria" id="ABF10867">
        <property type="protein sequence ID" value="ABF10867"/>
        <property type="gene ID" value="Rmet_3999"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3999"/>
    <dbReference type="eggNOG" id="COG4774">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008287_9_4_4"/>
    <dbReference type="OMA" id="HEDEDYW"/>
    <dbReference type="OrthoDB" id="169533at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009279">
        <property type="term" value="C:cell outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015344">
        <property type="term" value="F:siderophore uptake transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038023">
        <property type="term" value="F:signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.130.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.170.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012910">
        <property type="entry name" value="Plug_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037066">
        <property type="entry name" value="Plug_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011662">
        <property type="entry name" value="Secretin/TonB_short_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039423">
        <property type="entry name" value="TonB-dep_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000531">
        <property type="entry name" value="TonB-dep_rcpt_b-brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036942">
        <property type="entry name" value="TonB_rcpt_b-brl_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010105">
        <property type="entry name" value="TonB_sidphr_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32552">
        <property type="entry name" value="PTHR32552"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07715">
        <property type="entry name" value="Plug"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07660">
        <property type="entry name" value="STN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00593">
        <property type="entry name" value="TonB_dep_Rec"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00965">
        <property type="entry name" value="STN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01783">
        <property type="entry name" value="TonB-siderophor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0998">Cell outer membrane</keyword>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0614">Plasmid</keyword>
    <keyword evidence="6 10" id="KW-0675">Receptor</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="4 8" id="KW-0798">TonB box</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="STN" evidence="9">
        <location>
            <begin position="73"/>
            <end position="124"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009810"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003357"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00965"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ABF10867.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="821" mass="89363" checksum="0741EF97B99F870E" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="60" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LGA4</accession>
    <name>Q1LGA4_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_3954</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10822.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518461.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3954"/>
    <dbReference type="EnsemblBacteria" id="ABF10822">
        <property type="protein sequence ID" value="ABF10822"/>
        <property type="gene ID" value="Rmet_3954"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3954"/>
    <dbReference type="eggNOG" id="ENOG5031WHT">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_126950_0_0_4"/>
    <dbReference type="OMA" id="IHFVLGA"/>
    <dbReference type="OrthoDB" id="1556128at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="21"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="68"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="109"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="140"/>
            <end position="160"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF10822.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="167" mass="18348" checksum="DC5658FA63181001" modified="2006-05-30" version="1">MELHMHSHHYMRRLPDWTAAAVSGLAAGALLIVVEMFWSTMVAGVHPWGTTRMVAAIVLGRDVLQNSFFSVSTVAAALIIHFVLGAILGCVLCAIIAPFQLDSSVGMSLLVGAVFGLVVYMFNFYVMTSAFPWFVDQRGWHTLVGHLIFGMAIAICYWKLESRGVSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LGL9</accession>
    <name>Q1LGL9_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Heavy metal cation tricomponent efflux, membrane fusion protein HmvB (CzcB-like)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">hmvB</name>
        <name evidence="4" type="ordered locus">Rmet_3837</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="5">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="5">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10707.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3837"/>
    <dbReference type="EnsemblBacteria" id="ABF10707">
        <property type="protein sequence ID" value="ABF10707"/>
        <property type="gene ID" value="Rmet_3837"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3837"/>
    <dbReference type="eggNOG" id="COG0845">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018816_13_3_4"/>
    <dbReference type="OMA" id="VWNEDKT"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043602">
        <property type="entry name" value="CusB_dom_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032317">
        <property type="entry name" value="HlyD_D23"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006143">
        <property type="entry name" value="RND_pump_MFP"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00529">
        <property type="entry name" value="CusB_dom_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16576">
        <property type="entry name" value="HlyD_D23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01730">
        <property type="entry name" value="RND_mfp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0614">Plasmid</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HlyD_D23" evidence="3">
        <location>
            <begin position="80"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="domain" description="CusB_dom_1" evidence="2">
        <location>
            <begin position="315"/>
            <end position="379"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF16576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF10707.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="405" mass="43360" checksum="FC212FCC4FC01C79" modified="2006-05-30" version="1">MHRIPDMPLISRDSRFTRAGAARLAAVLSLCVLTLGACGKKEAPENDDEPKVAGQVIEYPASVRSLPGVVGQPVKSGGDRMLSMPGRLVWNEDKTVRVASPFAGRVMEILVQPGATVKAGQPLAMLTSPDFGSAQADARKASADSAVASKALARQRELYEAGVIAQKDLEQAQADNARAAADQQRTQAALRMYGASGSDGVNQRFALRSPIDGLVVERNINPGMELRPDQPPTAPLYLITDPTTLWAQLDAAEADLGHFRPGVTVQLASAAYPGETFTGTVVKIADYVDPSSRSVKVRLSVPNADRRLKAEMFVTAKMQAASFQGISVPSKAVFLADNKNYVFVRTTPNRFERREVKLGVTIPGTTEVVEGLKEGDVVITDGNLYLQDILRDATAVNAARATAKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LGR6</accession>
    <name>Q1LGR6_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Acyl-CoA dehydrogenase</fullName>
            <ecNumber evidence="9">1.3.99.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">Rmet_3790</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="10">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="10">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the acyl-CoA dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.3.99.-" evidence="9"/>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10660.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518308.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3790"/>
    <dbReference type="EnsemblBacteria" id="ABF10660">
        <property type="protein sequence ID" value="ABF10660"/>
        <property type="gene ID" value="Rmet_3790"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3790"/>
    <dbReference type="eggNOG" id="COG1960">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016513_2_1_4"/>
    <dbReference type="OMA" id="IEYHPAY"/>
    <dbReference type="OrthoDB" id="433393at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003995">
        <property type="term" value="F:acyl-CoA dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006089">
        <property type="entry name" value="Acyl-CoA_DH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006091">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036250">
        <property type="entry name" value="AcylCo_DH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009075">
        <property type="entry name" value="AcylCo_DH/oxidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009100">
        <property type="entry name" value="AcylCoA_DH/oxidase_NM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041504">
        <property type="entry name" value="AidB_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00441">
        <property type="entry name" value="Acyl-CoA_dh_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02770">
        <property type="entry name" value="Acyl-CoA_dh_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18158">
        <property type="entry name" value="AidB_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47203">
        <property type="entry name" value="SSF47203"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56645">
        <property type="entry name" value="SSF56645"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00073">
        <property type="entry name" value="ACYL_COA_DH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0274">FAD</keyword>
    <keyword evidence="3" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-0614">Plasmid</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AidB_N" evidence="8">
        <location>
            <begin position="27"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA_dh_M" evidence="7">
        <location>
            <begin position="198"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA_dh_1" evidence="6">
        <location>
            <begin position="305"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="562"/>
            <end position="581"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF02770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF18158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ABF10660.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="596" mass="65147" checksum="1DA02E0E223D199A" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="64" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LH77</accession>
    <name>Q1LH77_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Putative deoxygenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_3627</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10499.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518157.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3627"/>
    <dbReference type="EnsemblBacteria" id="ABF10499">
        <property type="protein sequence ID" value="ABF10499"/>
        <property type="gene ID" value="Rmet_3627"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3627"/>
    <dbReference type="eggNOG" id="COG5285">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_048953_3_0_4"/>
    <dbReference type="OMA" id="HHNCMMT"/>
    <dbReference type="OrthoDB" id="1070946at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008775">
        <property type="entry name" value="Phytyl_CoA_dOase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05721">
        <property type="entry name" value="PhyH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF10499.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="281" mass="30366" checksum="2CE8C77BAC23CF9E" modified="2006-05-30" version="1">MTAPQHHASGASASLDFDSSAPLADQAALASLAREFHAGGYIVLRKFASEATCAALEAVTRQQLAAAVPPVEFEADLGYPGAPATRESAGGHTVRRLRQAYGRDEVFRRWASDPAVVATVEALLGEPARLTLAHHNCVMTKHPHYGSQTGWHRDTRYWSFVKNDLITVWLALGDEDERNGVLRVIPGSHRAKLDPAQLDPAEFLIEAHPASQQLLKGTMPLALHRGDVLMFDSRLFHAAGRNDSEAVKLSVAFAYFGASNRPVAGTRSAEFGSVELPPTTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LHL6</accession>
    <name>Q1LHL6_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">ABC-type transporter, periplasmic component (Branched-chain amino acid transport)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Rmet_3488</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the leucine-binding protein family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10360.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008647384.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3488"/>
    <dbReference type="EnsemblBacteria" id="ABF10360">
        <property type="protein sequence ID" value="ABF10360"/>
        <property type="gene ID" value="Rmet_3488"/>
    </dbReference>
    <dbReference type="GeneID" id="60822594"/>
    <dbReference type="KEGG" id="rme:Rmet_3488"/>
    <dbReference type="eggNOG" id="COG0683">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027128_6_0_4"/>
    <dbReference type="OMA" id="PEYTKQG"/>
    <dbReference type="OrthoDB" id="776281at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006865">
        <property type="term" value="P:amino acid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028081">
        <property type="entry name" value="Leu-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000709">
        <property type="entry name" value="Leu_Ile_Val-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028082">
        <property type="entry name" value="Peripla_BP_I"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13458">
        <property type="entry name" value="Peripla_BP_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00337">
        <property type="entry name" value="LEUILEVALBP"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53822">
        <property type="entry name" value="SSF53822"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0029">Amino-acid transport</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193550" evidence="3">
        <location>
            <begin position="30"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="domain" description="Peripla_BP_6" evidence="4">
        <location>
            <begin position="31"/>
            <end position="356"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010062"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF13458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABF10360.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="383" mass="40314" checksum="0EA8224685D20EC3" modified="2006-05-30" version="1">MSSTFRKTAMTVAMAVAGLTAASAAFAQAQEIKLGFAGPMTGGQAQYGKDMQNGIVLAIEDMNATKPKIGGKDVKFVLVSEDDQADPKTGSVVAQKLVDAGIQGMLGHFNSGTSIPASMVYNRAGIPQIAMATAPEYTRQGFKTTFRMMTSDTQQGSVIGAFVVKKLGAKNIAIVDDRTAYGQGLADEFEKAAKAAGGKIVRREFTNDKAVDFKAVLTNIKRANPDIIFYGGAETQSAPMAKQVKELGMKAPLVSGEMSKTDNFLKLAGPAADGTVVSLAGLPLDQMPGGVAYEKKYEKRFGSKVQTYSPYAYDGATAMMTAMIKAGSADPARYLPVLAATNMQGVTTKTLAYDARGDLKDGGITVYKVADGKWTVLQTVGGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LHV4</accession>
    <name>Q1LHV4_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Permease Membrane protein mosC Major facilitator superfamily MFS_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">mosC</name>
        <name evidence="3" type="ordered locus">Rmet_3400</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10272.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3400"/>
    <dbReference type="EnsemblBacteria" id="ABF10272">
        <property type="protein sequence ID" value="ABF10272"/>
        <property type="gene ID" value="Rmet_3400"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3400"/>
    <dbReference type="eggNOG" id="COG2814">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035309_1_1_4"/>
    <dbReference type="OMA" id="FPMGMSA"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="SSF103473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="31"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="60"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="92"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="120"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="153"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="180"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="221"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="251"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="287"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="311"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="344"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="372"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF10272.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="400" mass="41027" checksum="AD6F1DD27F1F5E4F" modified="2006-05-30" version="1">MSEYSIESPARTETAPPPSPSAVSRATRGTMALFFANGATFATWGVHIPTIKARFGLSDAMLSFAMLAVAAGAIVAMGPVGRWVGRLGSARASMIGGVAFALLTLAIIAMPSFALLLPTLLIFGMANAAFDVGMNAQAAIVEASRTRPVMSALHGMFSLGGMIGAAFGGLMLSLGVPPLVHAALIASLTAGVAIGMTPGLLADHPVLPTADHHREHASRALWLLGLMAFLGLVGEGAMYDWTSVYMRDVAVAPLGWISFGYAAFSGGMACGRFGGDRLRARFGDARTLTGSAWLGFAGIVLAIAVPLPLAALIGFGLMGLGAANMVPIFFAAASRLPGVAAAEAIARVARFAYVGLLLGPVLIGGVAHLSDLRIGITVVALTMGYIALFGAKAARRYLIT</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LIC8</accession>
    <name>Q1LIC8_CUPMC</name>
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        <submittedName>
            <fullName evidence="2">ClpXP protease specificity-enhancing factor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">sspB</name>
        <name evidence="2" type="ordered locus">Rmet_3226</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
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            <taxon>Burkholderiaceae</taxon>
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        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10098.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517709.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3226"/>
    <dbReference type="EnsemblBacteria" id="ABF10098">
        <property type="protein sequence ID" value="ABF10098"/>
        <property type="gene ID" value="Rmet_3226"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3226"/>
    <dbReference type="eggNOG" id="COG2969">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_118425_1_0_4"/>
    <dbReference type="OMA" id="FQARFGG"/>
    <dbReference type="OrthoDB" id="1790783at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008233">
        <property type="term" value="F:peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.220">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007481">
        <property type="entry name" value="SspB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036760">
        <property type="entry name" value="SspB-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37486">
        <property type="entry name" value="PTHR37486"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04386">
        <property type="entry name" value="SspB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005276">
        <property type="entry name" value="SspB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101738">
        <property type="entry name" value="SSF101738"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="99"/>
            <end position="165"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF10098.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="165" mass="17931" checksum="608FBE71E83CA393" modified="2006-05-30" version="1">MSETSTKPYLIRAIYEWCTDNGFTPYIAVFVDGNTSVPREFVKNNEIVLNVSFDATSGLDMGNEWITFSARFGGVSRKIEVPVDNVLAIYARENGQGMAFPVERSIPETQAATERENNPPPKLASVDSDTPAPVTAEEGVSPDDDPTPPSVPRIGGKKPSLKVVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LIH5</accession>
    <name>Q1LIH5_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Bifunctional protein</fullName>
            <ecNumber evidence="2">2.6.1.85</ecNumber>
            <ecNumber evidence="2">4.1.3.27</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">trpG</name>
        <name evidence="2" type="ordered locus">Rmet_3179</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
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    <dbReference type="EC" id="4.1.3.27" evidence="2"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10051.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008647587.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3179"/>
    <dbReference type="MEROPS" id="C26.955"/>
    <dbReference type="EnsemblBacteria" id="ABF10051">
        <property type="protein sequence ID" value="ABF10051"/>
        <property type="gene ID" value="Rmet_3179"/>
    </dbReference>
    <dbReference type="GeneID" id="60825472"/>
    <dbReference type="KEGG" id="rme:Rmet_3179"/>
    <dbReference type="eggNOG" id="COG0512">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014340_1_2_4"/>
    <dbReference type="OMA" id="TEHGHAM"/>
    <dbReference type="OrthoDB" id="1561172at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046820">
        <property type="term" value="F:4-amino-4-deoxychorismate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004049">
        <property type="term" value="F:anthranilate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071704">
        <property type="term" value="P:organic substance metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="CDD" id="cd01743">
        <property type="entry name" value="GATase1_Anthranilate_Synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017926">
        <property type="entry name" value="GATASE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006221">
        <property type="entry name" value="TrpG/PapA_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00117">
        <property type="entry name" value="GATase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="SSF52317"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00566">
        <property type="entry name" value="trpG_papA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="2" id="KW-0456">Lyase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="GATase" evidence="1">
        <location>
            <begin position="4"/>
            <end position="185"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF10051.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="189" mass="20737" checksum="7AD0A43E28475A54" modified="2006-05-30" version="1">MLLMIDNYDSFTYNLVQYFGELGADVRTFRNDEITIEEIEALKPDHICVSPGPCSPHEAGISVPVLQHFAGKIPLLGVCLGHQAIGEAFGGKVIRAKQVMHGKVSTIETTQEGVFAGLPKHFDVTRYHSLAIERETLPDCLAVTAWTPDGEIMGVRHKTLAVEGVQFHPESILSEHGHALLANFIKAPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="110" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LIJ9</accession>
    <name>Q1LIJ9_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Dipeptide transporter ATP-binding component of ABC superfamily</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">dppD</name>
        <name evidence="10" type="ordered locus">Rmet_3155</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10027.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517638.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3155"/>
    <dbReference type="EnsemblBacteria" id="ABF10027">
        <property type="protein sequence ID" value="ABF10027"/>
        <property type="gene ID" value="Rmet_3155"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3155"/>
    <dbReference type="eggNOG" id="COG0444">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_23_4"/>
    <dbReference type="OMA" id="PYHTIGK"/>
    <dbReference type="OrthoDB" id="1101128at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015833">
        <property type="term" value="P:peptide transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013563">
        <property type="entry name" value="Oligopep_ABC_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08352">
        <property type="entry name" value="oligo_HPY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01727">
        <property type="entry name" value="oligo_HPY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 8 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-1278">Translocase</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ABC transporter" evidence="9">
        <location>
            <begin position="9"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="42"/>
            <end position="49"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ABF10027.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="332" mass="36174" checksum="256165DF964CB806" modified="2006-05-30" version="1">MTQPTLVVEHLKTQFFTRAGVARAVDDVSFTVGRGEIMGLVGESGSGKSMTGYSIMGLIDPPGKVVEGRIALTSRDGVTRDLRALTPAQQRDVRGNRIAMIFQDPMMTLNPVLRIDTQMIEAVRAHDDVSKAAARERARNALARVGIPSPDERLQAYPHQFSGGMRQRVAIAIALLNKPDLIIADEPTTALDVTIQGQILYEMQTLCRESGTALIWITHDLSVVAGLADKVCVMYAGKIVESGDVRQVLEAPRHPYTHGLIDSAPSRNPRGVPLRQIPGMTPSLLNLPSGCAFRERCPYATEICATEPPLELSPDGRQLRCFHPVHAQQEAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LIZ1</accession>
    <name>Q1LIZ1_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Cation/multidrug efflux system, membrane-fusion component (Modular protein)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">hmzB</name>
        <name evidence="4" type="ordered locus">Rmet_3012</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09885.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517531.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3012"/>
    <dbReference type="EnsemblBacteria" id="ABF09885">
        <property type="protein sequence ID" value="ABF09885"/>
        <property type="gene ID" value="Rmet_3012"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3012"/>
    <dbReference type="eggNOG" id="COG0845">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018816_13_0_4"/>
    <dbReference type="OMA" id="RNEQTQA"/>
    <dbReference type="OrthoDB" id="1174564at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032317">
        <property type="entry name" value="HlyD_D23"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006143">
        <property type="entry name" value="RND_pump_MFP"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16576">
        <property type="entry name" value="HlyD_D23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01730">
        <property type="entry name" value="RND_mfp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="17"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="domain" description="HlyD_D23" evidence="3">
        <location>
            <begin position="102"/>
            <end position="298"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF16576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF09885.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="407" mass="41805" checksum="B9C5C19C7C24FE34" modified="2006-05-30" version="1">MSFPTLKAAGTSSRTKTLALSAVGVAVLAAVGYGWWAWATQQTTVADKDASEAVAQPNAGGVHLNATQLRAQGVETALVKDAAVIPLDSLPAQTVAPLSASAQVVAPYGGVVTRVLVDEGAAVRQGQALARIQSKDVLASQADLARARTEAAVATAQARRDATLLAEGIIPATRNEQTQARAAAAQSTLQEANGALARLRPVSGGQAGEYELLAPLSGRVMRRHLSLGQAVAPLDIAFIVAQPGPLDVSVAVPLRWRSDLHPGLEVRLPDGTIARVTAVGGDTDLSSQSLRVRARVDADQAGADRYAAGQQISVALLLPAPQGTLSVPSSALLPAGSAHVVYVAEPSSQDKHGDLRVRAVPVRLLGQDESDASSAVRAVSPDTAPLAAGMQVVVRGTALLKSMIPLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="113" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LJ95</accession>
    <name>Q1LJ95_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 5">DNA mismatch repair protein MutL</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5 9" type="primary">mutL</name>
        <name evidence="9" type="ordered locus">Rmet_2908</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">This protein is involved in the repair of mismatches in DNA. It is required for dam-dependent methyl-directed DNA mismatch repair. May act as a 'molecular matchmaker', a protein that promotes the formation of a stable complex between two or more DNA-binding proteins in an ATP-dependent manner without itself being part of a final effector complex.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the DNA mismatch repair MutL/HexB family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09781.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517455.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2908"/>
    <dbReference type="EnsemblBacteria" id="ABF09781">
        <property type="protein sequence ID" value="ABF09781"/>
        <property type="gene ID" value="Rmet_2908"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2908"/>
    <dbReference type="eggNOG" id="COG0323">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004131_4_2_4"/>
    <dbReference type="OMA" id="AHERIMY"/>
    <dbReference type="OrthoDB" id="764332at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032300">
        <property type="term" value="C:mismatch repair complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140664">
        <property type="term" value="F:ATP-dependent DNA damage sensor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030983">
        <property type="term" value="F:mismatched DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006298">
        <property type="term" value="P:mismatch repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1540.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00149">
        <property type="entry name" value="DNA_mis_repair"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014762">
        <property type="entry name" value="DNA_mismatch_repair_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020667">
        <property type="entry name" value="DNA_mismatch_repair_MutL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002099">
        <property type="entry name" value="DNA_mismatch_repair_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013507">
        <property type="entry name" value="DNA_mismatch_S5_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038973">
        <property type="entry name" value="MutL/Mlh/Pms"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014790">
        <property type="entry name" value="MutL_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042120">
        <property type="entry name" value="MutL_C_dimsub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042121">
        <property type="entry name" value="MutL_C_regsub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037198">
        <property type="entry name" value="MutL_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10073">
        <property type="entry name" value="PTHR10073"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01119">
        <property type="entry name" value="DNA_mis_repair"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08676">
        <property type="entry name" value="MutL_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01340">
        <property type="entry name" value="DNA_mis_repair"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00853">
        <property type="entry name" value="MutL_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF118116">
        <property type="entry name" value="SSF118116"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="SSF54211"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="SSF55874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00585">
        <property type="entry name" value="mutl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00058">
        <property type="entry name" value="DNA_MISMATCH_REPAIR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0227">DNA damage</keyword>
    <keyword evidence="4 5" id="KW-0234">DNA repair</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA_mis_repair" evidence="8">
        <location>
            <begin position="220"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="domain" description="MutL_C" evidence="7">
        <location>
            <begin position="468"/>
            <end position="611"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="409"/>
            <end position="462"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00021975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM01340"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ABF09781.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="655" mass="71196" checksum="31EACCA067B960B3" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LJB9</accession>
    <name>Q1LJB9_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Riboflavin biosynthesis protein</fullName>
        </recommendedName>
        <domain>
            <recommendedName>
                <fullName evidence="12">Riboflavin kinase</fullName>
                <ecNumber evidence="12">2.7.1.26</ecNumber>
            </recommendedName>
            <alternativeName>
                <fullName evidence="12">Flavokinase</fullName>
            </alternativeName>
        </domain>
        <domain>
            <recommendedName>
                <fullName evidence="12">FMN adenylyltransferase</fullName>
                <ecNumber evidence="12">2.7.7.2</ecNumber>
            </recommendedName>
            <alternativeName>
                <fullName evidence="12">FAD pyrophosphorylase</fullName>
            </alternativeName>
            <alternativeName>
                <fullName evidence="12">FAD synthase</fullName>
            </alternativeName>
        </domain>
    </protein>
    <gene>
        <name evidence="14" type="primary">ribF</name>
        <name evidence="14" type="ordered locus">Rmet_2884</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Catalyzes the phosphorylation of riboflavin to FMN followed by the adenylation of FMN to FAD.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2 12">
            <text>ATP + FMN + H(+) = diphosphate + FAD</text>
            <dbReference type="Rhea" id="RHEA:17237"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
            <dbReference type="EC" id="2.7.7.2"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 12">
            <text>ATP + riboflavin = ADP + FMN + H(+)</text>
            <dbReference type="Rhea" id="RHEA:14357"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57986"/>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.26"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="4 12">Cofactor biosynthesis; FAD biosynthesis; FAD from FMN: step 1/1.</text>
    </comment>
    <comment type="pathway">
        <text evidence="5 12">Cofactor biosynthesis; FMN biosynthesis; FMN from riboflavin (ATP route): step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the ribF family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.26" evidence="12"/>
    <dbReference type="EC" id="2.7.7.2" evidence="12"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09757.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2884"/>
    <dbReference type="EnsemblBacteria" id="ABF09757">
        <property type="protein sequence ID" value="ABF09757"/>
        <property type="gene ID" value="Rmet_2884"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2884"/>
    <dbReference type="eggNOG" id="COG0196">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_048437_0_1_4"/>
    <dbReference type="OMA" id="HRGHQAI"/>
    <dbReference type="UniPathway" id="UPA00276">
        <property type="reaction ID" value="UER00406"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00277">
        <property type="reaction ID" value="UER00407"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003919">
        <property type="term" value="F:FMN adenylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008531">
        <property type="term" value="F:riboflavin kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006747">
        <property type="term" value="P:FAD biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009398">
        <property type="term" value="P:FMN biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009231">
        <property type="term" value="P:riboflavin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02064">
        <property type="entry name" value="FAD_synthetase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004821">
        <property type="entry name" value="Cyt_trans-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015864">
        <property type="entry name" value="FAD_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023468">
        <property type="entry name" value="Riboflavin_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002606">
        <property type="entry name" value="Riboflavin_kinase_bac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015865">
        <property type="entry name" value="Riboflavin_kinase_bac/euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023465">
        <property type="entry name" value="Riboflavin_kinase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22749">
        <property type="entry name" value="PTHR22749"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06574">
        <property type="entry name" value="FAD_syn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01687">
        <property type="entry name" value="Flavokinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004491">
        <property type="entry name" value="FAD_Synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00904">
        <property type="entry name" value="Flavokinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82114">
        <property type="entry name" value="SSF82114"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00125">
        <property type="entry name" value="cyt_tran_rel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00083">
        <property type="entry name" value="ribF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10 12" id="KW-0274">FAD</keyword>
    <keyword evidence="6 12" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="7 12" id="KW-0288">FMN</keyword>
    <keyword evidence="12 14" id="KW-0418">Kinase</keyword>
    <keyword evidence="9 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12 14" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 12 14" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Flavokinase" evidence="13">
        <location>
            <begin position="197"/>
            <end position="322"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000372"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001332"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00002121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00005201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRNR" id="PIRNR004491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM00904"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ABF09757.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="342" mass="37547" checksum="0B0E66F38D204B7E" modified="2006-05-30" version="1">MVCSYPPQKRSSAVLVKVFRGLPNAESRAPCALTIGNFDGVHRGHQSLLARARAAADARGLPLCVMTFEPHPREFFAPEHAPTRIALLRDKLDALRRNGVDRVVVEHFNAHFAAQSPQAFVENVLWHGLHARWVLVGDDFRFGAKRAGDFSYLQEAGRQFGFDVEQMGSVSEGGIRISSSAVRQALAGGDLEHARRLLGHGYAISGHVIHGRKLGRDLGFPTLNLRISHKRPAVNGIFVVQVHGIADHPVPGVASIGVRPTVEDAGRVLLEVHLFDFNENLYGKLIRVEFMKKLRDEKRYDSLEALTAAIAKDAADARAFFGQTAPDAGRRDFATSATDRIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="97" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LJL6</accession>
    <name>Q1LJL6_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="9">ABC-type cobalamin transporter, ATPase component(BtuD)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">btuD</name>
        <name evidence="9" type="ordered locus">Rmet_2787</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09660.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517358.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2787"/>
    <dbReference type="EnsemblBacteria" id="ABF09660">
        <property type="protein sequence ID" value="ABF09660"/>
        <property type="gene ID" value="Rmet_2787"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2787"/>
    <dbReference type="eggNOG" id="COG1120">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_11_4"/>
    <dbReference type="OMA" id="PIGRTHV"/>
    <dbReference type="OrthoDB" id="1752365at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="1" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-1278">Translocase</keyword>
    <feature type="domain" description="ABC transporter" evidence="8">
        <location>
            <begin position="14"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="46"/>
            <end position="53"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ABF09660.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="269" mass="28944" checksum="C6D136D8ED8616A0" modified="2006-05-30" version="1">MSHWSEPLTDCPQLTLRQLGLHAGSRVLLDGLSMSIGAGQLWCIVGPNGVGKSTLMGVLAGLRAPDSGAAEVDEIAVTQIAPATLARQRAYLPQAVHDTFSMLVEDAVRIGRHPHLSGWGWGQRDDDRIVDDVIDALDLEPLRGRDVLTLSGGERQRVSLAAALAQQAPLLLLDEPVAHLDLRHQIMVLDLLQRLTRSGRHAVVVIVHDLTLAHRYATHALLMAEDGHALHGPAHEVLTPAQCSHALRTPIISISDGTHTALIPDGNQT</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="97" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LJZ7</accession>
    <name>Q1LJZ7_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Alkyl hydroperoxide reductase C</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Peroxiredoxin</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">Thioredoxin peroxidase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">Rmet_2654</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peroxiredoxin family. AhpC/Prx1 subfamily.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the peroxiredoxin family. Prx6 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09529.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517227.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2654"/>
    <dbReference type="EnsemblBacteria" id="ABF09529">
        <property type="protein sequence ID" value="ABF09529"/>
        <property type="gene ID" value="Rmet_2654"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2654"/>
    <dbReference type="eggNOG" id="COG0450">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_042529_4_2_4"/>
    <dbReference type="OMA" id="EKVMILP"/>
    <dbReference type="OrthoDB" id="892697at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008379">
        <property type="term" value="F:thioredoxin peroxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03016">
        <property type="entry name" value="PRX_1cys"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000866">
        <property type="entry name" value="AhpC/TSA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024706">
        <property type="entry name" value="Peroxiredoxin_AhpC-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019479">
        <property type="entry name" value="Peroxiredoxin_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045020">
        <property type="entry name" value="PRX_1cys"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10417">
        <property type="entry name" value="1-cysPrx_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00578">
        <property type="entry name" value="AhpC-TSA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000239">
        <property type="entry name" value="AHPC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="SSF52833"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0049">Antioxidant</keyword>
    <keyword evidence="5 11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3 11" id="KW-0575">Peroxidase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thioredoxin" evidence="10">
        <location>
            <begin position="3"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="active site" description="Cysteine sulfenic acid (-SOH) intermediate" evidence="9">
        <location>
            <position position="45"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009796"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00017462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00025719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00032077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00032824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR000239-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ABF09529.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="212" mass="23939" checksum="06284115D6D3181A" modified="2006-05-30" version="1">MALRLGDIAPDFEQESSEGRIRFHDWLGDGWGVLFSHPADYTPVCTTELGLTARLKEEFAKRNVKAIALSVDSVESHHGWIQDINDTQSTTVNFPILADGDRKVSELYDMIHPNANETLTVRSLFVIDPKKKVRLIITYPASTGRNFNEILRVIDSLQLTDNHSVATPGNWQDGDDVVIVPSLKDEEVIRQKFPKGYKAVRPYLRLTPQPNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LK65</accession>
    <name>Q1LK65_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Extra-cytoplasmic Solute Receptor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">bug</name>
        <name evidence="4" type="ordered locus">Rmet_2584</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Periplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the UPF0065 (bug) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09461.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517164.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2584"/>
    <dbReference type="EnsemblBacteria" id="ABF09461">
        <property type="protein sequence ID" value="ABF09461"/>
        <property type="gene ID" value="Rmet_2584"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2584"/>
    <dbReference type="eggNOG" id="COG3181">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045683_0_0_4"/>
    <dbReference type="OMA" id="PIMYADL"/>
    <dbReference type="OrthoDB" id="447734at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042597">
        <property type="term" value="C:periplasmic space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005064">
        <property type="entry name" value="BUG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042100">
        <property type="entry name" value="Bug_dom1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42928">
        <property type="entry name" value="PTHR42928"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03401">
        <property type="entry name" value="TctC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017082">
        <property type="entry name" value="YflP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0675">Receptor</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193619" evidence="3">
        <location>
            <begin position="36"/>
            <end position="337"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF09461.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="337" mass="35870" checksum="D5A1BD00EC0109C8" modified="2006-05-30" version="1">MQYRSHRHQRTHRRLALSTLAVAALLPFVPATAQAQANYPDKPIRLVVPFPAGGPTDTAARIIGQKMSETLKQPIVIDNRPGASGTIGTETVAKATPDGYTIVMLATPTLLAPYLLPRKGYDIFKDFAPIGNAYELPIVMVVNPHALPDVTNLQQYIAKAKANPGQMNYTSAGNGSFGHLSTELLKNLGHFDVQHVPYKGSAPAMSDVLAGQVPMMFSDMIAALPHIKAGKLRAIAVGSSKRVSFAPEVKTVAEQGFPGFDAASWGGLLAPAGTSKEIVAKLSAALKGALADPTVEQKMLGAGTVASYLPSDQFATRMRNDYAKWGKVIQDNGIKSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-12-02" version="64" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LKE5</accession>
    <name>Q1LKE5_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Gluconate 2-dehydrogenase subunit 3 (GADH subunit 3) (GA 2-DH subunit 3)</fullName>
            <ecNumber evidence="1">1.1.99.3</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_2504</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EC" id="1.1.99.3" evidence="1"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09381.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2504"/>
    <dbReference type="EnsemblBacteria" id="ABF09381">
        <property type="protein sequence ID" value="ABF09381"/>
        <property type="gene ID" value="Rmet_2504"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2504"/>
    <dbReference type="eggNOG" id="ENOG502Z7SX">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_065508_0_0_4"/>
    <dbReference type="OMA" id="MDWVERN"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033717">
        <property type="term" value="F:gluconate 2-dehydrogenase (acceptor) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027056">
        <property type="entry name" value="Gluconate_2DH_su3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13618">
        <property type="entry name" value="Gluconate_2-dh3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF09381.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="273" mass="30017" checksum="7FD8D61066AA7798" modified="2006-05-30" version="1">MTVSLMVQGSTTCFVFFPKALRSTPHEKTTMSQEQEPRRRFFRQMLAVVPAASVATGAAISQSACSESPGPQSANAGNVGPYKPNYFTAGEWKFVQAAVDRLIPEDELGPGGLKAGVPEYIDRQMDTPYGHGKLWYIQGPFHPDVPAELGFQMNLAPRDIYRLGVKACDEYCKKQYGGKAFADLSKEDQESVLGQMEHAKISFDAVPARTFFSYLLNNTKEGFFADPIYGGNKGMVGWKMVGFPGARADFADWIDQPGVKYPYGPVSIDGRRG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2007-07-10" modified="2022-08-03" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LKG0</accession>
    <name>MDH_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Malate dehydrogenase</fullName>
            <ecNumber evidence="2">1.1.1.37</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">mdh</name>
        <name type="ordered locus">Rmet_2489</name>
    </gene>
    <organism>
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Catalyzes the reversible oxidation of malate to oxaloacetate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>(S)-malate + NAD(+) = H(+) + NADH + oxaloacetate</text>
            <dbReference type="Rhea" id="RHEA:21432"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15589"/>
            <dbReference type="ChEBI" id="CHEBI:16452"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.1.1.37"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the LDH/MDH superfamily. MDH type 2 family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.37" evidence="2"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09366.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008649349.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
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    <dbReference type="AlphaFoldDB" id="Q1LKG0"/>
    <dbReference type="SMR" id="Q1LKG0"/>
    <dbReference type="STRING" id="266264.Rmet_2489"/>
    <dbReference type="EnsemblBacteria" id="ABF09366">
        <property type="protein sequence ID" value="ABF09366"/>
        <property type="gene ID" value="Rmet_2489"/>
    </dbReference>
    <dbReference type="GeneID" id="60821036"/>
    <dbReference type="KEGG" id="rme:Rmet_2489"/>
    <dbReference type="eggNOG" id="COG0039">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_040727_2_0_4"/>
    <dbReference type="OMA" id="TKGMERG"/>
    <dbReference type="OrthoDB" id="870724at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030060">
        <property type="term" value="F:L-malate dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006108">
        <property type="term" value="P:malate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.110.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01517">
        <property type="entry name" value="Malate_dehydrog_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001557">
        <property type="entry name" value="L-lactate/malate_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022383">
        <property type="entry name" value="Lactate/malate_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001236">
        <property type="entry name" value="Lactate/malate_DH_N"/>
    </dbReference>
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        <property type="entry name" value="Lactate_DH/Glyco_Ohase_4_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010945">
        <property type="entry name" value="Malate_DH_type2"/>
    </dbReference>
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        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23382">
        <property type="entry name" value="PTHR23382"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02866">
        <property type="entry name" value="Ldh_1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00056">
        <property type="entry name" value="Ldh_1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000102">
        <property type="entry name" value="Lac_mal_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56327">
        <property type="entry name" value="SSF56327"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01759">
        <property type="entry name" value="MalateDH-SF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0520">NAD</keyword>
    <keyword id="KW-0560">Oxidoreductase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0816">Tricarboxylic acid cycle</keyword>
    <feature type="initiator methionine" description="Removed" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000294402" description="Malate dehydrogenase">
        <location>
            <begin position="2"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="2">
        <location>
            <position position="188"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="12"/>
            <end position="18"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="93"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="99"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="106"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="113"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="130"/>
            <end position="132"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="132"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="163"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01517"/>
        </source>
    </evidence>
    <sequence length="327" mass="35163" checksum="1E417F56B57623E7" modified="2006-05-30" version="1">MAKAPMRVAVTGAAGQIGYSLLFRIANGDMLGKDQPVILQLLDLPQAQAAVKGVVMELEDCAFPLLAGVVITDDPKVAFKDADVALLVGARPRSKGMERKDLLEANAQIFTVQGKALDEVASRDVKVLVVGNPANTNAYIAMKSAPNLKRENFTAMLRLDHNRALSQIAAKTGKPVSSIEKMFVWGNHSPTMYADYRYATVDGKSVKDMINDPVWNNDVFLPTVGKRGAAIIEARGLSSAASAANAAIDHVHDWVLGSNGKVVTMGIPSNGEYGIPNDVMFGYPVTTANGKYEIVKGLEIDAYSQEKINITLKELEEERAGVQHLLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="98" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LKH3</accession>
    <name>Q1LKH3_CUPMC</name>
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        <submittedName>
            <fullName evidence="9">Leucine/isoleucine/valine transporter subunit, ATP-binding component of ABC superfamily</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">livF1</name>
        <name evidence="9" type="ordered locus">Rmet_2476</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09353.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517064.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2476"/>
    <dbReference type="EnsemblBacteria" id="ABF09353">
        <property type="protein sequence ID" value="ABF09353"/>
        <property type="gene ID" value="Rmet_2476"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2476"/>
    <dbReference type="eggNOG" id="COG0410">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_2_4"/>
    <dbReference type="OMA" id="NDMFEAE"/>
    <dbReference type="OrthoDB" id="1475841at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015658">
        <property type="term" value="F:branched-chain amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030660">
        <property type="entry name" value="ABC_branched_ATPase_LivF/BraG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF039137">
        <property type="entry name" value="ABC_branched_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="2" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="1" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-1278">Translocase</keyword>
    <feature type="domain" description="ABC transporter" evidence="8">
        <location>
            <begin position="2"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="34"/>
            <end position="41"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ABF09353.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="233" mass="25359" checksum="8099E01DFF33052D" modified="2006-05-30" version="1">MLKLENVHTHYGAVEALSGVSIEVNKGEIVTLIGSNGAGKTTLMMTVCGTPRASSGRVSFEGQDITRRNTHEIMRMGMAISPEGRRVFPSLTVLENLKMGAFFASRHEIEEGIEHVFKLFPRLNQRASQRAGTMSGGEQQMLAIGRALMSKPRLLLLDEPTLGLAPLIIAQIFDIIRTIRDEGVTVFLVEQNANKALQAADRGYVLETGKVVLADTGQNLLANDRIRAAYLGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="100" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LKJ6</accession>
    <name>Q1LKJ6_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transcriptional Regulator, AraC family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_2453</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
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        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09330.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517045.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2453"/>
    <dbReference type="EnsemblBacteria" id="ABF09330">
        <property type="protein sequence ID" value="ABF09330"/>
        <property type="gene ID" value="Rmet_2453"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2453"/>
    <dbReference type="eggNOG" id="COG2207">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_88_16_4"/>
    <dbReference type="OMA" id="HAYDPHD"/>
    <dbReference type="OrthoDB" id="1268974at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003313">
        <property type="entry name" value="AraC-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037923">
        <property type="entry name" value="HTH-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018060">
        <property type="entry name" value="HTH_AraC"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02311">
        <property type="entry name" value="AraC_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12833">
        <property type="entry name" value="HTH_18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00342">
        <property type="entry name" value="HTH_ARAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51215">
        <property type="entry name" value="SSF51215"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01124">
        <property type="entry name" value="HTH_ARAC_FAMILY_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH araC/xylS-type" evidence="1">
        <location>
            <begin position="175"/>
            <end position="272"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS01124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF09330.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="279" mass="31548" checksum="584C1AB7613D11ED" modified="2006-05-30" version="1">MSPQSWIDLRQDASTGIETIRAHFTGHAYDPHWHDSYLVGYTEQGIQQFHCRREVQRSTPGKVFTLEPGEIHDGYAVAPEGFTYSMLYLDAQWMERELRAVFEDAPAHCQPGFAQTLREDPALISAIGSAYTVLQQPELRIVRQSALDALLAQLTAHLHWRAHHAFDPRLPLVAQRARDYLHDHLDHDIGLDDLAAISGVDRYRLTRAFKAAFGIAPHAYLVQMRLARARHLLARGATPADVAVALGFADQSHLGRWFRRAYGLTPAHYRKRCSNLPDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="72" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LKQ7</accession>
    <name>Q1LKQ7_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Cytochrome c-type biogenesis protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">cycL</name>
        <name evidence="6" type="ordered locus">Rmet_2392</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Possible subunit of a heme lyase.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the CcmH/CycL/Ccl2/NrfF family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09269.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_003149833.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2392"/>
    <dbReference type="EnsemblBacteria" id="ABF09269">
        <property type="protein sequence ID" value="ABF09269"/>
        <property type="gene ID" value="Rmet_2392"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2392"/>
    <dbReference type="eggNOG" id="COG3088">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_107187_2_1_4"/>
    <dbReference type="OMA" id="MIAADMR"/>
    <dbReference type="OrthoDB" id="1984854at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.640">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005616">
        <property type="entry name" value="CcmH/CycL/Ccl2/NrfF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038297">
        <property type="entry name" value="CcmH/CycL/NrfF/Ccl2_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03918">
        <property type="entry name" value="CcmH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 5" id="KW-0349">Heme</keyword>
    <keyword evidence="4 5" id="KW-0408">Iron</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5011023615" description="Cytochrome c-type biogenesis protein" evidence="5">
        <location>
            <begin position="20"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="96"/>
            <end position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU364112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABF09269.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="151" mass="17237" checksum="5682AFAE9DDB1A31" modified="2006-05-30" version="1">MILWRVALFFFLFTAPVWASDQAAEDRMLGIASELRCLVCQNESIAASQADLAADLRQQIREQIRAGKSDDDIRTYMVERYGDFVLYRPPLKPTTMLLWFGPFVVLALGIVVLVMNLRSRNRKPKHAALNEEERRHADALLAPPHNVLDET</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LL52</accession>
    <name>Q1LL52_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Putative diguanylate cyclase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Rmet_2245</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09124.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516948.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2245"/>
    <dbReference type="EnsemblBacteria" id="ABF09124">
        <property type="protein sequence ID" value="ABF09124"/>
        <property type="gene ID" value="Rmet_2245"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2245"/>
    <dbReference type="eggNOG" id="COG2199">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039310_2_1_4"/>
    <dbReference type="OMA" id="RVYADHN"/>
    <dbReference type="OrthoDB" id="1635706at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01949">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033417">
        <property type="entry name" value="CHASE8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000160">
        <property type="entry name" value="GGDEF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003660">
        <property type="entry name" value="HAMP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029787">
        <property type="entry name" value="Nucleotide_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17152">
        <property type="entry name" value="CHASE8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00990">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00267">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00304">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55073">
        <property type="entry name" value="SSF55073"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00254">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50887">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50885">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="27"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="157"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="domain" description="HAMP" evidence="2">
        <location>
            <begin position="189"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="domain" description="GGDEF" evidence="3">
        <location>
            <begin position="285"/>
            <end position="419"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF09124.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="426" mass="46102" checksum="1169879ECFFB9290" modified="2006-05-30" version="1">MNWPGRPPARDDAVPQRPTLYRALRRIHLGVALIAVFTVGISLTALGLTALRVYADHNLRLVARSIAYTVEAPVVFGDAAAAADALSVIAASEDVTRVIVYDRDHQPMTVWQRPGHEQFPALRRVAGDLLISGAVEQPILHEAQQVGSVRVAGDPVALLHFLLQGTLGLGACLLLTALVAHYMSRRMLRDVVNPLRNLMRVAHAVRSERAFGQRMPPARIEELNALSEDFNVLLDELEAWQNQLQHENRSLAHRASHDSLTGLLNRAAFTEALDLAVRTAAARNQYAAVLYLDSDDFKGINDRYGHAAGDAVLVAVGQRLRGCLREGDSVARLGGDEFAVLVNALRAPEDAVRIAEHILNAMATPVCLADGTTIFAPLSIGLAVYPLHARNGTALLHSADAAMYGAKRQSGATWHSAESAPPEPHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="107" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LL63</accession>
    <name>Q1LL63_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="11">ABC-type transporter, ATPase component</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">Rmet_2234</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09113.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516940.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2234"/>
    <dbReference type="EnsemblBacteria" id="ABF09113">
        <property type="protein sequence ID" value="ABF09113"/>
        <property type="gene ID" value="Rmet_2234"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2234"/>
    <dbReference type="eggNOG" id="COG3839">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_1_4"/>
    <dbReference type="OMA" id="FSEPPIN"/>
    <dbReference type="OrthoDB" id="1200451at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043190">
        <property type="term" value="C:ATP-binding cassette (ABC) transporter complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015408">
        <property type="term" value="F:ABC-type ferric iron transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd03259">
        <property type="entry name" value="ABC_Carb_Solutes_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015853">
        <property type="entry name" value="ABC_transpr_FbpC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008995">
        <property type="entry name" value="Mo/tungstate-bd_C_term_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013611">
        <property type="entry name" value="Transp-assoc_OB_typ2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08402">
        <property type="entry name" value="TOBE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50331">
        <property type="entry name" value="SSF50331"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-1278">Translocase</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ABC transporter" evidence="10">
        <location>
            <begin position="3"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="353"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="35"/>
            <end position="42"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ABF09113.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="374" mass="39773" checksum="002DCED5D47D9939" modified="2006-05-30" version="1">MQLSLEGIAQHVGSQAYLYSMSLAPVPGAVTILLGTTQAGKTSLMRVMAGLDKPTAGRVLVDGADVTGVPVRERNVSMVYQQFINYPSLRVFDNIASPLRLRRDRQADVDARVRSLAGRLHIEHLLDRYPSELSGGQQQRVALARALAKNAPLMLLDEPLVNLDYKLREELREELTQLFAQGDATVIYATTEPAEALLLGGHTAVLDAGELLQYGPTPEVFHYPASLRVARAFSDPPMNLLEGVLGHGRVVLAGNISIPMPDVAHADGPVTVGVRAGALRLDAVPQAVAVPGRVALAELSGSDTFVHVDTPAGNLVAQLTGVRDLELGAQLSLHIEPAQIYLFNADGDRIHAPRRPGMSPASAGPVAAITESGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="96" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LL77</accession>
    <name>Q1LL77_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">ABC nitrate/sulfonate/bicarbonate family transporter, inner membrane subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Rmet_2220</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cell membrane</location>
            <topology evidence="4">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the binding-protein-dependent transport system permease family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09099.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516927.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2220"/>
    <dbReference type="EnsemblBacteria" id="ABF09099">
        <property type="protein sequence ID" value="ABF09099"/>
        <property type="gene ID" value="Rmet_2220"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2220"/>
    <dbReference type="eggNOG" id="COG0600">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046113_0_1_4"/>
    <dbReference type="OMA" id="AYANMYA"/>
    <dbReference type="OrthoDB" id="1531811at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="SSF161098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2 4" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="20"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="63"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="96"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="156"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="189"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="214"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="256"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="306"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="5">
        <location>
            <begin position="150"/>
            <end position="331"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABF09099.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="347" mass="37320" checksum="C2294FCD68A00B68" modified="2006-05-30" version="1">MTTNQSTLNARLAQPAPRTVAPRVTVWPTGIVAALAWGVFGAVTWRWPNKMAGFNDWAYTEELGIVVLGVSAALLLLALAGGYASRSGPLQHAVATLRAAGPWLVALPLVFTAWEILTAKMAILPTPFFAPPQALIEVYLDDWQRLGDSAFNTLKLLGLGVGYGALAGFLIGVSIGWSRRIGYWIHPVLRVLGPLPSTALLPLTFYFFPSSYSAAVFLIALATAFPVAVLTWSGVAGVNKSYYDVARTMGASGWFLVLRVAIPAALPQVFVGLFMGLGASFSVLVTAEMMGVKSGLGWYLTWAQGWASYVNMYAALIVMALLFSGVITLLFAVRDRALSWQKGTVKW</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="72" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LL83</accession>
    <name>Q1LL83_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_2214</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09093.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516921.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2214"/>
    <dbReference type="EnsemblBacteria" id="ABF09093">
        <property type="protein sequence ID" value="ABF09093"/>
        <property type="gene ID" value="Rmet_2214"/>
    </dbReference>
    <dbReference type="GeneID" id="60821213"/>
    <dbReference type="KEGG" id="rme:Rmet_2214"/>
    <dbReference type="eggNOG" id="COG4318">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_090314_0_0_4"/>
    <dbReference type="OMA" id="PYHEFRW"/>
    <dbReference type="OrthoDB" id="579692at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd16390">
        <property type="entry name" value="ParB_N_Srx_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036086">
        <property type="entry name" value="ParB/Sulfiredoxin_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014956">
        <property type="entry name" value="ParBc_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016932">
        <property type="entry name" value="UCP029669"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08857">
        <property type="entry name" value="ParBc_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF029669">
        <property type="entry name" value="UCP029669"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110849">
        <property type="entry name" value="SSF110849"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF09093.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="198" mass="21976" checksum="318229BDB09ACD15" modified="2006-05-30" version="1">MPKIRESLIHDLHPTQLTVGMIEVQDKKTHLASLKPKDQQAFMEAHPIPAVEGPGGKLYITDHHHLGRAALEAGLTSGFFMVEADLSTSAPGDFWGEMDKNQWVHPLDENGVRHCYTLIPSHLEKLIDDPYRSLAGYVRDAGGYQKTPTAFAEFVWADFFRRHIAVEDLKADFQAAVKCAKVLAASKWASGLPGFQAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-10-07" version="53" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LLS7</accession>
    <name>Q1LLS7_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_2020</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08899.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2020"/>
    <dbReference type="EnsemblBacteria" id="ABF08899">
        <property type="protein sequence ID" value="ABF08899"/>
        <property type="gene ID" value="Rmet_2020"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2020"/>
    <dbReference type="HOGENOM" id="CLU_1667954_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="122"/>
            <end position="158"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF08899.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="158" mass="15798" checksum="1C3136E4C3993FE2" modified="2006-05-30" version="1">MKKLGGGNASTGAVRSRHIVGPGILSVAGLAILDGIRSPRRGGAMPDAAAGAFAHVTADLSGEDMKLSRRKPKSDALPGKVLAVLAQAPAPLSAGRVAKALAGVSRPTVTETLRAMAEAGAVLTGRTEGGQTRYAAPTSDPAQGDDTLRAGALRPGGW</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LM53</accession>
    <name>Q1LM53_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Transcriptional regulator (LysR family)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">aaeR</name>
        <name evidence="4" type="ordered locus">Rmet_1894</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the LysR transcriptional regulatory family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08773.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516625.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1894"/>
    <dbReference type="EnsemblBacteria" id="ABF08773">
        <property type="protein sequence ID" value="ABF08773"/>
        <property type="gene ID" value="Rmet_1894"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1894"/>
    <dbReference type="eggNOG" id="COG0583">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039613_16_2_4"/>
    <dbReference type="OMA" id="HSHNTEL"/>
    <dbReference type="OrthoDB" id="1661500at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005119">
        <property type="entry name" value="LysR_subst-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000847">
        <property type="entry name" value="Tscrpt_reg_HTH_LysR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00126">
        <property type="entry name" value="HTH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03466">
        <property type="entry name" value="LysR_substrate"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50931">
        <property type="entry name" value="HTH_LYSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH lysR-type" evidence="3">
        <location>
            <begin position="10"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="305"/>
            <end position="324"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF08773.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="324" mass="35053" checksum="2CB269DBDBEB5904" modified="2006-05-30" version="1">MRKNLDYGLLHAMTAFLRVVDAGSFTAAAAQMALTTAQVSRLVSELEKRLQAKLLQRTTRRLALTSVGERYADQCRSILELVAEAESGVGGANVEPDGRLRVRCMAAFGERYVVPLVSMYCARYPKVAVEYGTSQYAPDLIAEGVDASVYLAQRLSDSNLVAQALGETYGLLCAAPSYLKRRGTPKSVEELAAHACLRIANPSTTPDWTLTDGTRSFTLDAGGPLVGDNPGVVLHAASSGLGIALLPIFSAVDHIRNGELRVVLPAWRSPAIGVYLMLPSRKFLEAKTRAWIELLKTELPTALQRDAGHPAAATRAKPRRYSSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LM88</accession>
    <name>Q1LM88_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Leucine/isoleucine/valine ABC-like transporter, ATPase subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">livG7</name>
        <name evidence="9" type="ordered locus">Rmet_1859</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08738.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516586.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1859"/>
    <dbReference type="EnsemblBacteria" id="ABF08738">
        <property type="protein sequence ID" value="ABF08738"/>
        <property type="gene ID" value="Rmet_1859"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1859"/>
    <dbReference type="eggNOG" id="COG0411">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_2_4"/>
    <dbReference type="OMA" id="QITNIFH"/>
    <dbReference type="OrthoDB" id="1179151at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032823">
        <property type="entry name" value="BCA_ABC_TP_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12399">
        <property type="entry name" value="BCA_ABC_TP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="1" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-1278">Translocase</keyword>
    <feature type="domain" description="ABC transporter" evidence="8">
        <location>
            <begin position="6"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="38"/>
            <end position="45"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ABF08738.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="259" mass="28467" checksum="AE617B26397276C6" modified="2006-05-30" version="1">MTTPALELSDVRKRFGQTEIIRGVTLSIGKGERHALIGPNGAGKSTTFNLISGRFAPTSGSVKLNGQSIAGLRPFEINRMGLSRSFQITNIFHRLSVFENLRCAVLWSLGYKYSFWHRLSELRDARERAEEVLELIGMEHRRNTQAGLLTYAEQRALEIGITIAGGAEVILLDEPTAGMSRSESDHAVELIRKVTVGKTLVMVEHDMSVVFGLADRISVLVYGEVIATDTPEAIRNNRKVKEAYLGTTLDEAATEGAHG</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="51" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LMN1</accession>
    <name>Q1LMN1_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_1714</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08595.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1714"/>
    <dbReference type="EnsemblBacteria" id="ABF08595">
        <property type="protein sequence ID" value="ABF08595"/>
        <property type="gene ID" value="Rmet_1714"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1714"/>
    <dbReference type="HOGENOM" id="CLU_2092735_0_0_4"/>
    <dbReference type="OMA" id="QAYRDNC"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="47"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="98"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="119"/>
            <end position="133"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF08595.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="140" mass="14533" checksum="52523E4F820B745D" modified="2006-05-30" version="1">MTCTAPSNSPSLSVRRHIGAPMEMPNAVLLTVALTLGLPAVALAQSTSPAQGNAAAAISDAVQEGANPYRPPSASSVSPEEARRAKCEELKNQFAAGAHGPNSYTSPGMATQNAQGRAVPKIERDKSRDALEDTYRANCT</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LMU7</accession>
    <name>Q1LMU7_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Small-conductance mechanosensitive channel</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Rmet_1646</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Mechanosensitive channel that participates in the regulation of osmotic pressure changes within the cell, opening in response to stretch forces in the membrane lipid bilayer, without the need for other proteins. Contributes to normal resistance to hypoosmotic shock. Forms an ion channel of 1.0 nanosiemens conductance with a slight preference for anions.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Homoheptamer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cell inner membrane</location>
            <topology evidence="7">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the MscS (TC 1.A.23) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08529.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516374.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1646"/>
    <dbReference type="EnsemblBacteria" id="ABF08529">
        <property type="protein sequence ID" value="ABF08529"/>
        <property type="gene ID" value="Rmet_1646"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1646"/>
    <dbReference type="eggNOG" id="COG3264">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032048_1_0_4"/>
    <dbReference type="OMA" id="ITYMRPF"/>
    <dbReference type="OrthoDB" id="1903165at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="F:mechanosensitive ion channel activity"/>
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        <property type="entry name" value="MscS_channel"/>
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        <property type="entry name" value="MscS_channel_C"/>
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        <property type="entry name" value="MscS_dom_sf"/>
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        <property type="entry name" value="PTHR30221"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00924">
        <property type="entry name" value="MS_channel"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SSF50182"/>
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        <property type="entry name" value="SSF82689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0407">Ion channel</keyword>
    <keyword evidence="7" id="KW-0406">Ion transport</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="7" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="168"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="215"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="279"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="324"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="353"/>
            <end position="379"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU369025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ABF08529.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="557" mass="60235" checksum="F7F510617C69CD71" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="105" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LMW6</accession>
    <name>Q1LMW6_CUPMC</name>
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        <submittedName>
            <fullName evidence="5">DNA-binding response regulator in two-component regulatory system</fullName>
        </submittedName>
    </protein>
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        <name evidence="5" type="ordered locus">Rmet_1627</name>
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    <organism evidence="5 6">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
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            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
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        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="ABF08510.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1627"/>
    <dbReference type="EnsemblBacteria" id="ABF08510">
        <property type="protein sequence ID" value="ABF08510"/>
        <property type="gene ID" value="Rmet_1627"/>
    </dbReference>
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        <property type="taxonomic scope" value="Bacteria"/>
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    <dbReference type="OrthoDB" id="1020672at2"/>
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        <property type="component" value="Chromosome"/>
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        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="CheY-like_superfamily"/>
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        <property type="entry name" value="OmpR/PhoB-type_DNA-bd"/>
    </dbReference>
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        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
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        <property type="entry name" value="WalR-like"/>
    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Trans_reg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="OMPR_PHOB"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
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        <location>
            <begin position="2"/>
            <end position="116"/>
        </location>
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        <location>
            <begin position="124"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="OmpR/PhoB-type" evidence="2">
        <location>
            <begin position="124"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="1">
        <location>
            <position position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABF08510.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="230" mass="25444" checksum="D6D24ED25C01B2FE" modified="2006-05-30" version="1">MRILLAEDNLKLAESLVDSLSQSGFAVDCMHDGASADHLLTTQDYALAILDIGMPRLDGLEVLRRLRQRRNPVPVLILTAHGSVEDRVRGLDLGADDYLAKPFALNELEARARALIRRSHGHDRTALECGPLRYDSVSRAFTLHGELLQLTGRERAVLEVLLMRDGRAVNKAALSEKIFGIDESVNADAIEIYVYRLRKKLEGSGVSIVTLRGLGYLLESRDTATSDARQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LMX3</accession>
    <name>Q1LMX3_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Acetolactate synthase isozyme II large subunit</fullName>
            <ecNumber evidence="6">2.2.1.6</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">ilvG</name>
        <name evidence="6" type="ordered locus">Rmet_1620</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the TPP enzyme family.</text>
    </comment>
    <dbReference type="EC" id="2.2.1.6" evidence="6"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08503.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516349.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1620"/>
    <dbReference type="EnsemblBacteria" id="ABF08503">
        <property type="protein sequence ID" value="ABF08503"/>
        <property type="gene ID" value="Rmet_1620"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1620"/>
    <dbReference type="eggNOG" id="COG0028">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013748_3_4_4"/>
    <dbReference type="OMA" id="QIDRTDR"/>
    <dbReference type="OrthoDB" id="391134at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003984">
        <property type="term" value="F:acetolactate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030976">
        <property type="term" value="F:thiamine pyrophosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044281">
        <property type="term" value="P:small molecule metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029035">
        <property type="entry name" value="DHS-like_NAD/FAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012000">
        <property type="entry name" value="Thiamin_PyroP_enz_cen_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012001">
        <property type="entry name" value="Thiamin_PyroP_enz_TPP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045229">
        <property type="entry name" value="TPP_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011766">
        <property type="entry name" value="TPP_enzyme-bd_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18968">
        <property type="entry name" value="PTHR18968"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02775">
        <property type="entry name" value="TPP_enzyme_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00205">
        <property type="entry name" value="TPP_enzyme_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02776">
        <property type="entry name" value="TPP_enzyme_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52467">
        <property type="entry name" value="SSF52467"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="SSF52518"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0786">Thiamine pyrophosphate</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="TPP_enzyme_N" evidence="5">
        <location>
            <begin position="8"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="domain" description="TPP_enzyme_M" evidence="3">
        <location>
            <begin position="195"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="domain" description="TPP_enzyme_C" evidence="4">
        <location>
            <begin position="391"/>
            <end position="540"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007812"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU362132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF02775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF02776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABF08503.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="567" mass="61528" checksum="2AF2C8A4EF1902BA" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="106" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LN77</accession>
    <name>Q1LN77_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 7 10">Phosphoglycerate kinase</fullName>
            <ecNumber evidence="2 7 10">2.7.2.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">pgk1</name>
        <name evidence="7" type="synonym">pgk</name>
        <name evidence="11" type="ordered locus">Rmet_1516</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 7 10">
            <text>(2R)-3-phosphoglycerate + ATP = (2R)-3-phospho-glyceroyl phosphate + ADP</text>
            <dbReference type="Rhea" id="RHEA:14801"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57604"/>
            <dbReference type="ChEBI" id="CHEBI:58272"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.2.3"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="7">Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 2/5.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7 10">Belongs to the phosphoglycerate kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.7.2.3" evidence="2 7 10"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08399.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516259.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1516"/>
    <dbReference type="EnsemblBacteria" id="ABF08399">
        <property type="protein sequence ID" value="ABF08399"/>
        <property type="gene ID" value="Rmet_1516"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1516"/>
    <dbReference type="eggNOG" id="COG0126">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025427_0_2_4"/>
    <dbReference type="OrthoDB" id="462069at2"/>
    <dbReference type="UniPathway" id="UPA00109">
        <property type="reaction ID" value="UER00185"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004618">
        <property type="term" value="F:phosphoglycerate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006096">
        <property type="term" value="P:glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1260">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00145">
        <property type="entry name" value="Phosphoglyc_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001576">
        <property type="entry name" value="Phosphoglycerate_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015911">
        <property type="entry name" value="Phosphoglycerate_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015824">
        <property type="entry name" value="Phosphoglycerate_kinase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036043">
        <property type="entry name" value="Phosphoglycerate_kinase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11406">
        <property type="entry name" value="PTHR11406"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00162">
        <property type="entry name" value="PGK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000724">
        <property type="entry name" value="Pgk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00477">
        <property type="entry name" value="PHGLYCKINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53748">
        <property type="entry name" value="SSF53748"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00111">
        <property type="entry name" value="PGLYCERATE_KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="5 7 10" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7 10" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Substrate binding" evidence="7 8">
        <location>
            <begin position="25"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="7 8">
        <location>
            <begin position="64"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7 8">
        <location>
            <position position="41"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7 8">
        <location>
            <position position="118"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7 8">
        <location>
            <position position="151"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7 9">
        <location>
            <position position="202"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7 9">
        <location>
            <position position="324"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7 9">
        <location>
            <begin position="350"/>
            <end position="353"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR000724-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR000724-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ABF08399.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="396" mass="40955" checksum="A75A738C7C2915D5" modified="2006-05-30" version="1">MNVLRFADLVAQGRVCGQRVFIRADLNVPQDDAGNITEDTRIRASVLCIRMALDAGAAVMVTSHLGRPTEGEFKPEDSLAPVAARLAALLGRPVPLKSNWVGGVDVQPGEVVLLENCRLNKGEKKNSEALARQMAALTDIFVHDAFGTAHRAEASTYGIAQFAPVACAGPLLAAEIDAIGKALAAPKRPLVAIVAGSKVSTKLTILQSLAKNVDGLIVGGGIANTFLLAQGLPIGKSLAEPDLVDEARAVIDAMKARGAEVPIPVDVVCAKAFKADAPATVKAVAEVDDDDLILDIGPQTATLLAHKLAAAGTIVWNGPVGVFEFDAFANGTKTIARAIAASDAFSIAGGGDTLAAIAKYGIDKDVGYISTGGGAFLEVLEGKTLPAFEILARRAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="52" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LNI0</accession>
    <name>Q1LNI0_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_1413</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08296.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516168.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1413"/>
    <dbReference type="EnsemblBacteria" id="ABF08296">
        <property type="protein sequence ID" value="ABF08296"/>
        <property type="gene ID" value="Rmet_1413"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1413"/>
    <dbReference type="HOGENOM" id="CLU_1092893_0_0_4"/>
    <dbReference type="OMA" id="RYLVLRM"/>
    <dbReference type="OrthoDB" id="1360503at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF08296.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="245" mass="27180" checksum="C021C44870103F06" modified="2006-05-30" version="1">MTHPTHGSWTPVRKDFVDPASTRCHARNAKGGRHHGFPEGHAYILRGPDGHEYPFGPECAREVLAVPEALDQIPDYTERDAVKRIDADLPAPVRRKPSAAELELARRNAATRYVVLRMEKVAAVPRVQPTVLFPALDELYQRIATGGTLTNAQVQRVLAIERSKATPPKLKSTNLLDVYTAHVKLEWLIAGANRVDQIRFLRSLHDWLARHLVLSAAQIAAAGIVMHPHAFRSAWPSEGGPAELF</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-12-02" version="67" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LNJ5</accession>
    <name>Q1LNJ5_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_1398</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08281.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1398"/>
    <dbReference type="EnsemblBacteria" id="ABF08281">
        <property type="protein sequence ID" value="ABF08281"/>
        <property type="gene ID" value="Rmet_1398"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1398"/>
    <dbReference type="eggNOG" id="COG3782">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_053324_1_0_4"/>
    <dbReference type="OMA" id="GPGCHDR"/>
    <dbReference type="OrthoDB" id="1029598at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015003">
        <property type="entry name" value="DUF1853"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08907">
        <property type="entry name" value="DUF1853"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF08281.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="369" mass="40931" checksum="843CF75317342BEC" modified="2006-05-30" version="1">MRDLKPDLAQGIAPGGPALTGADGQMPLWRRAPSQRTRDLAWSSLSPPLLSRLPDGADVVRVAQWPAGVRAAWQDWLAQADPSSLPETITELSHHADANDGRSLRLGRHAERLLHFALDHAPGIELLAANVPIRRANGHGVQTLGELDVVWRDPAVMETVHWEMAAKFYLMVDGIEDRWLDLRAFVGPNLVDRLGDKLDHIVRRQLPLGRTAEAKAALGHAIDRSEVYLLGWLFYRDGVTPPGLDVLGIAPDHQRGWWSTLDAWMGRASERAGQGSGAARWCRLQRADWLSGARVPEAETESAEVLHAVLTERFTDAHPDHGWRRDAPVMVCELEPAGTDQPGIWVERSRGFVVPPGWEARAIERARRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="70" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LNK2</accession>
    <name>Q1LNK2_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">VOC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_1391</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08274.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008649113.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1391"/>
    <dbReference type="EnsemblBacteria" id="ABF08274">
        <property type="protein sequence ID" value="ABF08274"/>
        <property type="gene ID" value="Rmet_1391"/>
    </dbReference>
    <dbReference type="GeneID" id="60822147"/>
    <dbReference type="KEGG" id="rme:Rmet_1391"/>
    <dbReference type="eggNOG" id="COG0346">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1853996_0_0_4"/>
    <dbReference type="OMA" id="YERHWTP"/>
    <dbReference type="OrthoDB" id="1984437at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.180.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029068">
        <property type="entry name" value="Glyas_Bleomycin-R_OHBP_Dase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041581">
        <property type="entry name" value="Glyoxalase_6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037523">
        <property type="entry name" value="VOC"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18029">
        <property type="entry name" value="Glyoxalase_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54593">
        <property type="entry name" value="SSF54593"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51819">
        <property type="entry name" value="VOC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="VOC" evidence="1">
        <location>
            <begin position="1"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF08274.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="136" mass="15173" checksum="F4039C12CD0FE3E3" modified="2006-05-30" version="1">MELLINIDVDDLERGIGFYETGLGMRLARTLFDGTVAEMHGACAPVFLLAKPPGTFATTRSDSRRDYARHWTPVHLDFVVESLEPAIERALGAGAELEDWPRDFDWGRQAMLSDPFGHGLCFIEWKGDGYGANAER</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LNQ8</accession>
    <name>Q1LNQ8_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Cyclohexanol dehydrogenase (Protein belongs to CMGI-2)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">chnA</name>
        <name evidence="1" type="ordered locus">Rmet_1335</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08218.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1335"/>
    <dbReference type="EnsemblBacteria" id="ABF08218">
        <property type="protein sequence ID" value="ABF08218"/>
        <property type="gene ID" value="Rmet_1335"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1335"/>
    <dbReference type="eggNOG" id="COG1028">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010194_1_0_4"/>
    <dbReference type="OMA" id="LTTHIAC"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00061">
        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF08218.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="262" mass="26506" checksum="09BB942DB5A52C6F" modified="2006-05-30" version="1">MNQSNPEGGRFTMKYIFKGDSALVSGAGSGIGEATAHLLAINGVQVVASDIDFEAARRVTQAIIAAGGRAVAHEGNAAKPEDAGAAVQCALKHFGSLHLAFNNAGIAGELKPAGELAAEEWQRVIDTNLSGVQHAMRAQIPAILAAGGGAIVNMSSVLGLVGSADAPAYVAAKHGVTGLTRSAALAYSAQGVRINSLHPGYIETPLLKALDEATLAALKGLHAIGRLGRPEEIAHAACFLLSEGASFMCGSAMVVDGGYTAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LP93</accession>
    <name>Q1LP93_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Short-chain dehydrogenase/reductase SDR</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_1147</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08033.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008650518.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1147"/>
    <dbReference type="EnsemblBacteria" id="ABF08033">
        <property type="protein sequence ID" value="ABF08033"/>
        <property type="gene ID" value="Rmet_1147"/>
    </dbReference>
    <dbReference type="GeneID" id="60822016"/>
    <dbReference type="KEGG" id="rme:Rmet_1147"/>
    <dbReference type="eggNOG" id="COG1028">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010194_1_1_4"/>
    <dbReference type="OMA" id="GALPHMY"/>
    <dbReference type="OrthoDB" id="1294334at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00061">
        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF08033.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="261" mass="27624" checksum="D66CFFA2CBE6368E" modified="2006-05-30" version="1">MGLSINLEGKVALVTGASSGLGTRFSNVLAAAGAKVVLASRRVERLKELRASIEADGGSAHVVQLDVTDPDSIRAAVAHAETEAGAIDILVNNSGVSTTQKLTDVTADDFDFVFDTNTRGAFFVAQEVAKRMIARAKGAEKLGNPLPQARIVNIASVAGLKVLSQIGVYCMSKAAVVHMTKAMALEWARHGINTNAICPGYIDTEINHHHWDTDAGQKLIQMLPRKRLGQPEDLDGLLLLLASDASRFINGAVVTADDGMV</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LPC6</accession>
    <name>Q1LPC6_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Permeases of the major facilitator superfamily</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Rmet_1114</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08000.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515901.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1114"/>
    <dbReference type="EnsemblBacteria" id="ABF08000">
        <property type="protein sequence ID" value="ABF08000"/>
        <property type="gene ID" value="Rmet_1114"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1114"/>
    <dbReference type="eggNOG" id="COG2814">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001265_57_5_4"/>
    <dbReference type="OMA" id="LGGLMPC"/>
    <dbReference type="OrthoDB" id="626204at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001958">
        <property type="entry name" value="Tet-R_TetA/multi-R_MdtG"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01035">
        <property type="entry name" value="TCRTETA"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="SSF103473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="45"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="75"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="105"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="139"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="164"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="210"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="247"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="277"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="306"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="338"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="domain" description="MFS" evidence="6">
        <location>
            <begin position="8"/>
            <end position="388"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABF08000.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="395" mass="41000" checksum="7F43E509C1D438D8" modified="2006-05-30" version="1">MRPNRPQTLTVLLTTQAVVTTGLMTLVPILPLYLQAMPGTHGTDWSSVALAAPAVGALAVAPFTGIWCDRVGHRPVLLVSLVVFTVSVLILATSQHVAGFIVGRVLQGASAIGVMLTACIGHVSAPASRNWAFGLQESAVAAGALLGPVLGGVLQDVWSPRPMLFAVAALNALLTLALSTLLRDMPAHTAPVPSTGKRRPIRDVLMLRRWLAAACLSQAGAFALVNVFVLYLQQRAPHDALLASKAGTLHALGWLAAMFASPWWGARNDRVASGRHFLLAASGCAAAIALLPLATALWQIALLRVIHGALFAALALSVLAHCHRVLPAALHGTATGVARSAMTAGQLVGPLAVMVSLPLGGPNAALWVTAAMFAVSALLILPDRSPSFAPVHDAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LPF6</accession>
    <name>Q1LPF6_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_1084</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07970.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1084"/>
    <dbReference type="EnsemblBacteria" id="ABF07970">
        <property type="protein sequence ID" value="ABF07970"/>
        <property type="gene ID" value="Rmet_1084"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1084"/>
    <dbReference type="eggNOG" id="COG0457">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_068849_0_0_4"/>
    <dbReference type="OMA" id="TQSNPSY"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="SSF48452"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193302" evidence="2">
        <location>
            <begin position="50"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="53"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="209"/>
            <end position="251"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF07970.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="251" mass="26146" checksum="CF1BB7A3BA528CF0" modified="2006-05-30" version="1">MPRSITDRPYMSLPLPIPTIVSSRRGGPLSALASALAMAAALSAAPAFAQNGPLSLTPPPAAPTGPQSADPGMKPAQKAANEKRYDDAIAGFDRVLADNPRNAQARFQRAWAMAKAGREDDAIKAFAEMAQDFPELPEPHNNLALLYAKRGDLKRAEAELLLAIEARPDYALSYSNLGDVYRALAVQAYGDATRRNPGDKRAAASLRELQPAAASAPSAMPAPASGAAAVKGKAKAATSPVTPPASTPDAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LPI9</accession>
    <name>Q1LPI9_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Hydrolase, alpha/beta fold family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_1051</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07937.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515843.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1051"/>
    <dbReference type="ESTHER" id="ralme-q1lpi9">
        <property type="family name" value="6_AlphaBeta_hydrolase"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF07937">
        <property type="protein sequence ID" value="ABF07937"/>
        <property type="gene ID" value="Rmet_1051"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1051"/>
    <dbReference type="eggNOG" id="COG0596">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020336_22_0_4"/>
    <dbReference type="OMA" id="IFFAHAN"/>
    <dbReference type="OrthoDB" id="1498437at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12697">
        <property type="entry name" value="Abhydrolase_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="SSF53474"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AB hydrolase-1" evidence="1">
        <location>
            <begin position="5"/>
            <end position="258"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF07937.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="273" mass="30967" checksum="913627E29B1E308C" modified="2006-05-30" version="1">MRDILLFSHANGFPVPIYRKLFGALEDTFDIRAVERYGHNPKFPVTREWPHLVDELLGELDRPGRSGADAPKVWLVGHSLGGFLSLMAALRRPQRVRGVVMLDSPIIAGWKASLVRASQMLGLDEKPGPAAVTKNRRTHWPDTDAVWSHFQSKRNFKVWDPEVLRDYAEHGTEPTGVANERKLRFDREVEYWIYRTLPTSLGRKVARGAPIPVSFVAGTRSREVRQAGLGATRKLVGDRLRYIEGGHLYPMERPLETAGLVRDLIEEMRKSGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LPL3</accession>
    <name>Q1LPL3_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Iron-binding protein IscA</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">Iron-sulfur cluster assembly protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">iscA</name>
        <name evidence="6" type="ordered locus">Rmet_1027</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the HesB/IscA family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07913.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1027"/>
    <dbReference type="EnsemblBacteria" id="ABF07913">
        <property type="protein sequence ID" value="ABF07913"/>
        <property type="gene ID" value="Rmet_1027"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1027"/>
    <dbReference type="eggNOG" id="COG0316">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069054_5_1_4"/>
    <dbReference type="OMA" id="GYQYGMA"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.300.12">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000361">
        <property type="entry name" value="FeS_biogenesis"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016092">
        <property type="entry name" value="FeS_cluster_insertion"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017870">
        <property type="entry name" value="FeS_cluster_insertion_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035903">
        <property type="entry name" value="HesB-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011302">
        <property type="entry name" value="IscA_proteobacteria"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01521">
        <property type="entry name" value="Fe-S_biosyn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89360">
        <property type="entry name" value="SSF89360"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02011">
        <property type="entry name" value="IscA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00049">
        <property type="entry name" value="TIGR00049"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01152">
        <property type="entry name" value="HESB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fe-S_biosyn" evidence="5">
        <location>
            <begin position="9"/>
            <end position="109"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00014591"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00032050"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01521"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABF07913.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="113" mass="12577" checksum="9064FC1A38C76D11" modified="2006-05-30" version="1">MFDSKAKMISMTEKAAKHVARYLERRGKGVGLRVGVKTTGCSGLAYKLEYVDEVLPEDQVFETHGIKVIVDPKSLPYIDGTELDFAREGLNEGFKFNNPNVKDECGCGESFRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="51" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LPN7</accession>
    <name>Q1LPN7_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_1003</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07889.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF07889">
        <property type="protein sequence ID" value="ABF07889"/>
        <property type="gene ID" value="Rmet_1003"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1003"/>
    <dbReference type="HOGENOM" id="CLU_203774_0_0_4"/>
    <dbReference type="OMA" id="MTVFICL"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF07889.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="63" mass="6982" checksum="D605FE5E4B4B3D56" modified="2006-05-30" version="1">MDHFGASFGRFAMHKLPVVTLCLIAALSAMTVFICLSDTVAPRDTEYGSGKAVFKHYLVQYVR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-12-16" modified="2022-08-03" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LPW2</accession>
    <name>NUOB_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">NADH-quinone oxidoreductase subunit B</fullName>
            <ecNumber evidence="2">7.1.1.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">NADH dehydrogenase I subunit B</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="2">NDH-1 subunit B</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="2" type="primary">nuoB</name>
        <name type="ordered locus">Rmet_0928</name>
    </gene>
    <organism>
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>a quinone + 5 H(+)(in) + NADH = a quinol + 4 H(+)(out) + NAD(+)</text>
            <dbReference type="Rhea" id="RHEA:57888"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:24646"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:132124"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="2">Binds 1 [4Fe-4S] cluster.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">NDH-1 is composed of 14 different subunits. Subunits NuoB, C, D, E, F, and G constitute the peripheral sector of the complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell inner membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
            <orientation evidence="2">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the complex I 20 kDa subunit family.</text>
    </comment>
    <dbReference type="EC" id="7.1.1.-" evidence="2"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07814.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008643342.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q1LPW2"/>
    <dbReference type="SMR" id="Q1LPW2"/>
    <dbReference type="STRING" id="266264.Rmet_0928"/>
    <dbReference type="EnsemblBacteria" id="ABF07814">
        <property type="protein sequence ID" value="ABF07814"/>
        <property type="gene ID" value="Rmet_0928"/>
    </dbReference>
    <dbReference type="GeneID" id="57643151"/>
    <dbReference type="GeneID" id="60825415"/>
    <dbReference type="KEGG" id="rme:Rmet_0928"/>
    <dbReference type="eggNOG" id="COG0377">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_055737_7_3_4"/>
    <dbReference type="OMA" id="AGWVRKS"/>
    <dbReference type="OrthoDB" id="1904620at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008137">
        <property type="term" value="F:NADH dehydrogenase (ubiquinone) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048038">
        <property type="term" value="F:quinone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01356">
        <property type="entry name" value="NDH1_NuoB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006137">
        <property type="entry name" value="NADH_UbQ_OxRdtase-like_20kDa"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006138">
        <property type="entry name" value="NADH_UQ_OxRdtase_20Kd_su"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01058">
        <property type="entry name" value="Oxidored_q6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01957">
        <property type="entry name" value="nuoB_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01150">
        <property type="entry name" value="COMPLEX1_20K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0004">4Fe-4S</keyword>
    <keyword id="KW-0997">Cell inner membrane</keyword>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0408">Iron</keyword>
    <keyword id="KW-0411">Iron-sulfur</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0520">NAD</keyword>
    <keyword id="KW-0874">Quinone</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-1278">Translocase</keyword>
    <keyword id="KW-0813">Transport</keyword>
    <keyword id="KW-0830">Ubiquinone</keyword>
    <feature type="chain" id="PRO_0000358462" description="NADH-quinone oxidoreductase subunit B">
        <location>
            <begin position="1"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="37"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="38"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="102"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="132"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01356"/>
        </source>
    </evidence>
    <sequence length="160" mass="17635" checksum="481853353A83E48C" modified="2006-05-30" version="1">MAIEGVLNEGFVTTTADKLINWTRTGSLWPMTFGLACCAVEMMHAGAARYDMDRFGVIFRPSPRQSDVMIVAGTLCNKMAPALRKVYDQMAEPRWVISMGSCANGGGYYHYSYSVVRGCDRIVPVDIYVPGCPPTAEALIYGVIQLQNKIKRTNTIARKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LQ85</accession>
    <name>Q1LQ85_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Putative membrane-bound serine protease (Clpp class), S49 family</fullName>
            <ecNumber evidence="6">3.4.21.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Rmet_0805</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="3.4.21.-" evidence="6"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07691.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0805"/>
    <dbReference type="EnsemblBacteria" id="ABF07691">
        <property type="protein sequence ID" value="ABF07691"/>
        <property type="gene ID" value="Rmet_0805"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0805"/>
    <dbReference type="eggNOG" id="COG1030">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024619_1_0_4"/>
    <dbReference type="OMA" id="QMDTPGG"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008233">
        <property type="term" value="F:peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029045">
        <property type="entry name" value="ClpP/crotonase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002810">
        <property type="entry name" value="NfeD-like_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01957">
        <property type="entry name" value="NfeD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52096">
        <property type="entry name" value="SSF52096"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0645">Protease</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="291"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="326"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="363"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="399"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="domain" description="NfeD" evidence="5">
        <location>
            <begin position="406"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="177"/>
            <end position="196"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01957"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABF07691.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="491" mass="50756" checksum="DD5879CAECD7D95E" modified="2006-05-30" version="1">MAGRLHWKWRGHGIHCGPLPAMTLAPIRDWLARVRWLALGRALGLTLLAISGAHAQSASSASTAPAVPPVYVIPIRGAISPASASFAVHGIEQASKHGAQLIVIEMDTPGGLDKSMRDIIQAIIASPVPVATYVYPGGARAASAGTYILYASHIAAMAPGTNLGAASPVAIGIGGPKPEAGPAPASAPSSAPAAEDTMTRKQMHDASAYIRGLAQLRHRNADWGERAVREAVSLSADEAVAQHVADLIAADLPVLLRQVDGKRIEAAGAMRTLHTAGAPVVTLEPDWRSRLLAVITDPSVALILMMIGIYGLIFEFSTPGMVVPGIAGAICLLLGLFALQMLPVNYAGLALIALGVGCMTAELFLPTFGALGVGGIIAFAFGAVMLIDTDVPGFGVPLSLIATLSTVSAVFLFGMSMVLLRARRRPVVSGADALVGSIGVVIDDAPADGWATVHGEQWRIHSAAPLVRGQRVRVTARRGLVLDVTTAGDPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LQI6</accession>
    <name>Q1LQI6_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Cyanophycin synthetase</fullName>
            <ecNumber evidence="7">6.3.2.29</ecNumber>
            <ecNumber evidence="6">6.3.2.30</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Cyanophycin synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="primary">cphA2</name>
        <name evidence="12" type="ordered locus">Rmet_0704</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Catalyzes the ATP-dependent polymerization of arginine and aspartate to multi-L-arginyl-poly-L-aspartic acid (cyanophycin; a water-insoluble reserve polymer).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>[L-4-(L-arginin-2-N-yl)aspartate](n) + ATP + L-aspartate = [L-4-(L-arginin-2-N-yl)aspartate](n)-L-aspartate + ADP + H(+) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:13277"/>
            <dbReference type="Rhea" id="RHEA-COMP:13728"/>
            <dbReference type="Rhea" id="RHEA-COMP:13733"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:137986"/>
            <dbReference type="ChEBI" id="CHEBI:137990"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.2.29"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>[L-4-(L-arginin-2-N-yl)aspartate](n)-L-aspartate + ATP + L-arginine = [L-4-(L-arginin-2-N-yl)aspartate](n+1) + ADP + H(+) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:23888"/>
            <dbReference type="Rhea" id="RHEA-COMP:13732"/>
            <dbReference type="Rhea" id="RHEA-COMP:13733"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:32682"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:137986"/>
            <dbReference type="ChEBI" id="CHEBI:137990"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.2.30"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="5">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">In the C-terminal section; belongs to the MurCDEF family.</text>
    </comment>
    <dbReference type="EC" id="6.3.2.29" evidence="7"/>
    <dbReference type="EC" id="6.3.2.30" evidence="6"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07590.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515554.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0704"/>
    <dbReference type="EnsemblBacteria" id="ABF07590">
        <property type="protein sequence ID" value="ABF07590"/>
        <property type="gene ID" value="Rmet_0704"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0704"/>
    <dbReference type="eggNOG" id="COG1181">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016806_0_0_4"/>
    <dbReference type="OMA" id="LAYDRCQ"/>
    <dbReference type="OrthoDB" id="1861122at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071161">
        <property type="term" value="F:cyanophycin synthetase activity (L-arginine-adding)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071160">
        <property type="term" value="F:cyanophycin synthetase activity (L-aspartate-adding)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009059">
        <property type="term" value="P:macromolecule biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1190.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011761">
        <property type="entry name" value="ATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005479">
        <property type="entry name" value="CbamoylP_synth_lsu-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011810">
        <property type="entry name" value="Cya_phycin_syn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044019">
        <property type="entry name" value="Cyanophycin_syn_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036565">
        <property type="entry name" value="Mur-like_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004101">
        <property type="entry name" value="Mur_ligase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036615">
        <property type="entry name" value="Mur_ligase_C_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013221">
        <property type="entry name" value="Mur_ligase_cen"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02786">
        <property type="entry name" value="CPSase_L_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18921">
        <property type="entry name" value="Cyanophycin_syn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02875">
        <property type="entry name" value="Mur_ligase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08245">
        <property type="entry name" value="Mur_ligase_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53244">
        <property type="entry name" value="SSF53244"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53623">
        <property type="entry name" value="SSF53623"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02068">
        <property type="entry name" value="cya_phycin_syn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50975">
        <property type="entry name" value="ATP_GRASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="12" id="KW-0436">Ligase</keyword>
    <keyword evidence="10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ATP-grasp" evidence="11">
        <location>
            <begin position="224"/>
            <end position="474"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00000917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00003184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009060"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00013005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00031353"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ABF07590.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="883" mass="95726" checksum="16E0065AA36E4041" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="107" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LQJ7</accession>
    <name>Q1LQJ7_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Amino-acid transporter subunit ATP-binding component of ABC superfamily</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">aapP</name>
        <name evidence="10" type="ordered locus">Rmet_0693</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ABC transporter superfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07579.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515544.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0693"/>
    <dbReference type="EnsemblBacteria" id="ABF07579">
        <property type="protein sequence ID" value="ABF07579"/>
        <property type="gene ID" value="Rmet_0693"/>
    </dbReference>
    <dbReference type="GeneID" id="60825170"/>
    <dbReference type="KEGG" id="rme:Rmet_0693"/>
    <dbReference type="eggNOG" id="COG1126">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_22_4"/>
    <dbReference type="OMA" id="TIWFMDA"/>
    <dbReference type="OrthoDB" id="1191583at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015424">
        <property type="term" value="F:ABC-type amino acid transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030679">
        <property type="entry name" value="ABC_ATPase_HisP-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF039085">
        <property type="entry name" value="ABC_ATPase_HisP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-1278">Translocase</keyword>
    <feature type="domain" description="ABC transporter" evidence="9">
        <location>
            <begin position="2"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="34"/>
            <end position="41"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ABF07579.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="249" mass="27669" checksum="323D0B6CF176E551" modified="2006-05-30" version="1">MISIEHVSKWYGDYQALVDINESVAKAEVVVVCGPSGSGKSTLIRTLNRLEAIQKGRIVVNGQDVHARNVDVNALRSGIGFVFQQFNLFPHLTVLENCTLAPVRLKRMSTQEAKDFAMSLLERVGLPHKASAYPGQLSGGQQQRVAIARALAMKPPVMLFDEPTSALDPEMVNEVLQVMKGLARDGMTMVCVTHEMGFAREVADRVIFMDQGEVLERATPERFFQKPEHPRAQRFLADIRSPWESVIAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="111" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LQX8</accession>
    <name>Q1LQX8_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Acetate kinase</fullName>
            <ecNumber evidence="6">2.7.2.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Acetokinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">ackA2</name>
        <name evidence="6" type="synonym">ackA</name>
        <name evidence="8" type="ordered locus">Rmet_0562</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Catalyzes the formation of acetyl phosphate from acetate and ATP. Can also catalyze the reverse reaction.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>acetate + ATP = acetyl phosphate + ADP</text>
            <dbReference type="Rhea" id="RHEA:11352"/>
            <dbReference type="ChEBI" id="CHEBI:22191"/>
            <dbReference type="ChEBI" id="CHEBI:30089"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.2.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <cofactor evidence="6">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="6">Mg(2+). Can also accept Mn(2+).</text>
    </comment>
    <comment type="pathway">
        <text evidence="6">Metabolic intermediate biosynthesis; acetyl-CoA biosynthesis; acetyl-CoA from acetate: step 1/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 6 7">Belongs to the acetokinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.7.2.1" evidence="6"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07448.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515424.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="SMR" id="Q1LQX8"/>
    <dbReference type="STRING" id="266264.Rmet_0562"/>
    <dbReference type="EnsemblBacteria" id="ABF07448">
        <property type="protein sequence ID" value="ABF07448"/>
        <property type="gene ID" value="Rmet_0562"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0562"/>
    <dbReference type="eggNOG" id="COG0282">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020352_0_0_4"/>
    <dbReference type="OMA" id="IHYLYSI"/>
    <dbReference type="OrthoDB" id="537106at2"/>
    <dbReference type="UniPathway" id="UPA00340">
        <property type="reaction ID" value="UER00458"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008776">
        <property type="term" value="F:acetate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006085">
        <property type="term" value="P:acetyl-CoA biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006082">
        <property type="term" value="P:organic acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00020">
        <property type="entry name" value="Acetate_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004372">
        <property type="entry name" value="Ac/propionate_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000890">
        <property type="entry name" value="Aliphatic_acid_kin_short-chain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023865">
        <property type="entry name" value="Aliphatic_acid_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21060">
        <property type="entry name" value="PTHR21060"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00871">
        <property type="entry name" value="Acetate_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000722">
        <property type="entry name" value="Acetate_prop_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00471">
        <property type="entry name" value="ACETATEKNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="SSF53067"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00016">
        <property type="entry name" value="ackA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01075">
        <property type="entry name" value="ACETATE_KINASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01076">
        <property type="entry name" value="ACETATE_KINASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 6 7" id="KW-0418">Kinase</keyword>
    <keyword evidence="6" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6 7" id="KW-0808">Transferase</keyword>
    <feature type="active site" description="Proton donor/acceptor" evidence="6">
        <location>
            <position position="160"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="12"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="19"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="103"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="218"/>
            <end position="222"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="292"/>
            <end position="294"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="389"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="6">
        <location>
            <position position="191"/>
        </location>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="6">
        <location>
            <position position="251"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ABF07448.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="405" mass="42629" checksum="7C168BCB13FC7573" modified="2006-05-30" version="1">MTTAHPVILVVNAGSSSVKISIFSVPEAAHEDVEVLPAQLAHGQIEGIGVAPHIEGRLADGTVVADERFPVELVPDHDAAFRLIQARLQEGLRDTPPVAIGHRVVHGGMDFADAVRIDDNVIARLDALVPLAPLHQPHNLTAIRAVRAAAPDLLQVACFDTAFHAGHDPMAQLMALPYEYYEQGIRRFGFHGLSYAYIARRLKQVAPDLAEGRVIVAHFGNGASLCAMRGGKSVESTMGLTALDGMPMGTRCGTIDPGAVLWLAAQGMTPDAIQAMLYGQSGLKGISGVSSDMRALLASDTPRAKLAVDFYAYRAAQEIGKLAVTLGGLDGLVFTAGIGANSPDVRARVCAHLTDLFGISLDAAANGDNCQRISSDTSRVPVLALQTDEEGMIALSTAKILLNGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="61" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LQY9</accession>
    <name>Q1LQY9_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative hypothetical transmembrane protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_0551</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07437.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF07437">
        <property type="protein sequence ID" value="ABF07437"/>
        <property type="gene ID" value="Rmet_0551"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0551"/>
    <dbReference type="eggNOG" id="ENOG502Z9CN">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_034937_0_0_4"/>
    <dbReference type="OrthoDB" id="1881755at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019501">
        <property type="entry name" value="Peptidase_M30_hyicolysin"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10460">
        <property type="entry name" value="Peptidase_M30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193646" evidence="2">
        <location>
            <begin position="34"/>
            <end position="577"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="41"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="43"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF07437.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="577" mass="60324" checksum="6F2C10614B93C746" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2007-04-17" modified="2022-08-03" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LR89</accession>
    <name>YBEY_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Endoribonuclease YbeY</fullName>
            <ecNumber evidence="1">3.1.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">ybeY</name>
        <name type="ordered locus">Rmet_0451</name>
    </gene>
    <organism>
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Single strand-specific metallo-endoribonuclease involved in late-stage 70S ribosome quality control and in maturation of the 3' terminus of the 16S rRNA.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="1">Binds 1 zinc ion.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the endoribonuclease YbeY family.</text>
    </comment>
    <comment type="sequence caution" evidence="2">
        <conflict type="erroneous initiation">
            <sequence resource="EMBL-CDS" id="ABF07337" version="1"/>
        </conflict>
    </comment>
    <dbReference type="EC" id="3.1.-.-" evidence="1"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07337.1"/>
        <property type="status" value="ALT_INIT"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q1LR89"/>
    <dbReference type="SMR" id="Q1LR89"/>
    <dbReference type="STRING" id="266264.Rmet_0451"/>
    <dbReference type="EnsemblBacteria" id="ABF07337">
        <property type="protein sequence ID" value="ABF07337"/>
        <property type="gene ID" value="Rmet_0451"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0451"/>
    <dbReference type="eggNOG" id="COG0319">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1118710_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004521">
        <property type="term" value="F:endoribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.390.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00009">
        <property type="entry name" value="Endoribonucl_YbeY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023091">
        <property type="entry name" value="MetalPrtase_cat_dom_sf_prd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002036">
        <property type="entry name" value="YbeY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020549">
        <property type="entry name" value="YbeY_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02130">
        <property type="entry name" value="YbeY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00043">
        <property type="entry name" value="TIGR00043"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01306">
        <property type="entry name" value="UPF0054"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0255">Endonuclease</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0540">Nuclease</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0690">Ribosome biogenesis</keyword>
    <keyword id="KW-0698">rRNA processing</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <feature type="chain" id="PRO_0000284286" description="Endoribonuclease YbeY">
        <location>
            <begin position="1"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="156"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="160"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="166"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00009"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="197" mass="21530" checksum="A344EF107C2EC0FA" modified="2007-04-17" version="2">MVALLAEHTDTRQQAGLLVRPDNSDALSVAVTATPIVTTTGTPATPPALELDVQHGDGVSKRNGLPSRKQIEKWVKSALYADAALTVRFVDETEGRTLNRSYRGKDYATNVLTFAYAENDDDPVAGDIVLCCPVVESEAKAQKKSLEAHYAHLIVHGVLHAQGYEHDDDTEAEEMEAIETETLQALGFEDPYKPIRE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-01-15" modified="2022-05-25" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LRQ2</accession>
    <name>RL25_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">50S ribosomal protein L25</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">General stress protein CTC</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rplY</name>
        <name evidence="1" type="synonym">ctc</name>
        <name type="ordered locus">Rmet_0288</name>
    </gene>
    <organism>
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">This is one of the proteins that binds to the 5S RNA in the ribosome where it forms part of the central protuberance.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the 50S ribosomal subunit; part of the 5S rRNA/L5/L18/L25 subcomplex. Contacts the 5S rRNA. Binds to the 5S rRNA independently of L5 and L18.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the bacterial ribosomal protein bL25 family. CTC subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07174.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515177.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q1LRQ2"/>
    <dbReference type="SMR" id="Q1LRQ2"/>
    <dbReference type="STRING" id="266264.Rmet_0288"/>
    <dbReference type="EnsemblBacteria" id="ABF07174">
        <property type="protein sequence ID" value="ABF07174"/>
        <property type="gene ID" value="Rmet_0288"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0288"/>
    <dbReference type="eggNOG" id="COG1825">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_075939_0_1_4"/>
    <dbReference type="OMA" id="HVDFYEV"/>
    <dbReference type="OrthoDB" id="1673077at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008097">
        <property type="term" value="F:5S rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00495">
        <property type="entry name" value="Ribosomal_L25_TL5_CTC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.120.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.240.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01336">
        <property type="entry name" value="Ribosomal_L25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01334">
        <property type="entry name" value="Ribosomal_L25_CTC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020056">
        <property type="entry name" value="Rbsml_L25/Gln-tRNA_synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029751">
        <property type="entry name" value="Ribosomal_L25"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011035">
        <property type="entry name" value="Ribosomal_L25/Gln-tRNA_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020057">
        <property type="entry name" value="Ribosomal_L25_b-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037121">
        <property type="entry name" value="Ribosomal_L25_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001021">
        <property type="entry name" value="Ribosomal_L25_long"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020055">
        <property type="entry name" value="Ribosomal_L25_short"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01386">
        <property type="entry name" value="Ribosomal_L25p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14693">
        <property type="entry name" value="Ribosomal_TL5_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50715">
        <property type="entry name" value="SSF50715"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00731">
        <property type="entry name" value="bL25_bact_ctc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <feature type="chain" id="PRO_1000052924" description="50S ribosomal protein L25">
        <location>
            <begin position="1"/>
            <end position="203"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01334"/>
        </source>
    </evidence>
    <sequence length="203" mass="21782" checksum="8CE93565341F8621" modified="2006-05-30" version="1">MKVVAFERSVQGTGASRRLRNSGKTPGIIYGGAAEPKMIELDHNALWHALKKEAFHSSILDLEVAGKSEKALLRAFQMHPFKPLVLHVDFQRVSATEKIHVKVPLHFMNQETAPGVKLGHGIVSHILNDLEVSCLPADLPEFIEVDLAAAELNQTIHLSDLKLPKGVTAITHGDENPAIASITLPAGAQSSEAAEGGEETPAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="57" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LRU3</accession>
    <name>Q1LRU3_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">BFD-like (2Fe-2S)-binding region</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">bfd</name>
        <name evidence="2" type="ordered locus">Rmet_0247</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07133.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515143.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0247"/>
    <dbReference type="EnsemblBacteria" id="ABF07133">
        <property type="protein sequence ID" value="ABF07133"/>
        <property type="gene ID" value="Rmet_0247"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0247"/>
    <dbReference type="eggNOG" id="COG2906">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_159205_2_0_4"/>
    <dbReference type="OMA" id="YVCICNQ"/>
    <dbReference type="OrthoDB" id="1969696at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.1100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007419">
        <property type="entry name" value="BFD-like_2Fe2S-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041854">
        <property type="entry name" value="BFD-like_2Fe2S-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04324">
        <property type="entry name" value="Fer2_BFD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fer2_BFD" evidence="1">
        <location>
            <begin position="2"/>
            <end position="49"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF04324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF07133.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="101" mass="10628" checksum="E10B1BF04B5DCF19" modified="2006-05-30" version="1">MYVCICNQVTDHEIHGAAHLGVSTMDELAETLGVGTCCGRCRECAQQVLCEGVAAVKTVRSSSMATIKVVEISSCSTTTSHAIMDARDPAVANDRRTALVA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-01-15" modified="2022-08-03" version="92" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LS28</accession>
    <name>THIC_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Phosphomethylpyrimidine synthase</fullName>
            <ecNumber evidence="1">4.1.99.17</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Hydroxymethylpyrimidine phosphate synthase</fullName>
            <shortName evidence="1">HMP-P synthase</shortName>
            <shortName evidence="1">HMP-phosphate synthase</shortName>
            <shortName evidence="1">HMPP synthase</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Thiamine biosynthesis protein ThiC</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">thiC</name>
        <name type="ordered locus">Rmet_0162</name>
    </gene>
    <organism>
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the synthesis of the hydroxymethylpyrimidine phosphate (HMP-P) moiety of thiamine from aminoimidazole ribotide (AIR) in a radical S-adenosyl-L-methionine (SAM)-dependent reaction.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>5-amino-1-(5-phospho-beta-D-ribosyl)imidazole + S-adenosyl-L-methionine = 4-amino-2-methyl-5-(phosphooxymethyl)pyrimidine + 5'-deoxyadenosine + CO + formate + 3 H(+) + L-methionine</text>
            <dbReference type="Rhea" id="RHEA:24840"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15740"/>
            <dbReference type="ChEBI" id="CHEBI:17245"/>
            <dbReference type="ChEBI" id="CHEBI:17319"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:58354"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:137981"/>
            <dbReference type="EC" id="4.1.99.17"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="1">Binds 1 [4Fe-4S] cluster per subunit. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; thiamine diphosphate biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the ThiC family.</text>
    </comment>
    <dbReference type="EC" id="4.1.99.17" evidence="1"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07048.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008642628.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q1LS28"/>
    <dbReference type="SMR" id="Q1LS28"/>
    <dbReference type="STRING" id="266264.Rmet_0162"/>
    <dbReference type="EnsemblBacteria" id="ABF07048">
        <property type="protein sequence ID" value="ABF07048"/>
        <property type="gene ID" value="Rmet_0162"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0162"/>
    <dbReference type="eggNOG" id="COG0422">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013181_2_1_4"/>
    <dbReference type="OMA" id="TWELFRD"/>
    <dbReference type="OrthoDB" id="505395at2"/>
    <dbReference type="UniPathway" id="UPA00060"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016830">
        <property type="term" value="F:carbon-carbon lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009228">
        <property type="term" value="P:thiamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009229">
        <property type="term" value="P:thiamine diphosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.540">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00089">
        <property type="entry name" value="ThiC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037509">
        <property type="entry name" value="ThiC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025747">
        <property type="entry name" value="ThiC-associated_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038521">
        <property type="entry name" value="ThiC/Bza_core_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002817">
        <property type="entry name" value="ThiC/BzaA/B"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30557">
        <property type="entry name" value="PTHR30557"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13667">
        <property type="entry name" value="ThiC-associated"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01964">
        <property type="entry name" value="ThiC_Rad_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00407">
        <property type="entry name" value="phosphomethylpyrimidine_syntha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00190">
        <property type="entry name" value="thiC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0004">4Fe-4S</keyword>
    <keyword id="KW-0408">Iron</keyword>
    <keyword id="KW-0411">Iron-sulfur</keyword>
    <keyword id="KW-0456">Lyase</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword id="KW-0784">Thiamine biosynthesis</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <feature type="chain" id="PRO_1000004797" description="Phosphomethylpyrimidine synthase">
        <location>
            <begin position="1"/>
            <end position="625"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="237"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="266"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="295"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="331"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="351"/>
            <end position="353"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="392"/>
            <end position="395"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="431"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="435"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="458"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="499"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="579"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="582"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="587"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00089"/>
        </source>
    </evidence>
    <sequence length="625" mass="69358" checksum="6EFF5569259C33E9" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LSB2</accession>
    <name>Q1LSB2_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Predicted esterase of the alpha-beta hydrolase superfamily</fullName>
            <ecNumber evidence="4">3.1.-.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">rssA</name>
        <name evidence="4" type="ordered locus">Rmet_0078</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="3.1.-.-" evidence="4"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF06964.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515002.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0078"/>
    <dbReference type="EnsemblBacteria" id="ABF06964">
        <property type="protein sequence ID" value="ABF06964"/>
        <property type="gene ID" value="Rmet_0078"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0078"/>
    <dbReference type="eggNOG" id="COG1752">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_042893_0_0_4"/>
    <dbReference type="OMA" id="QVYRADS"/>
    <dbReference type="OrthoDB" id="1149616at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016035">
        <property type="entry name" value="Acyl_Trfase/lysoPLipase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002641">
        <property type="entry name" value="PNPLA_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01734">
        <property type="entry name" value="Patatin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52151">
        <property type="entry name" value="SSF52151"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51635">
        <property type="entry name" value="PNPLA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="1 2" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PNPLA" evidence="3">
        <location>
            <begin position="23"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="GXSXG" evidence="2">
        <location>
            <begin position="63"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="2">
        <location>
            <position position="65"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="2">
        <location>
            <position position="232"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF06964.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="418" mass="44528" checksum="7F29E6532CE933BF" modified="2006-05-30" version="1">MSALSAHSAEASARARQRQSTALVMMGGGARAAYQAGVLSGIAKIAARLGLAHCPLPFGIIAGTSAGAINGAGLAVGAADFRAATDKLSALWHSLHADDVYRTDVLRVGVSGARWLSSLAFGWVTRRSSPRALFDNAPLGSFLTELFEPERVQASLDAGVLQAFAVTALSYSTGRHVTFYQSHRRIHGWQRSQRMAVESAIGVDHLLASASIPFLFPAIPLELEGHHEWFGDGTMRQMSPLSPAIHLGASRILAIGAASRQRAGWFDTVPSGGYPSLAQVGGQALASIFLDGLQADIERLMHINRLLSRMPEMANDTEGWRPVQVMTISPSEPIEAIAAEHLNRLPRTVRAFLSPLGGTEARGAAFASYLLFEPEFTQALLALGERDAAAQGDQIAAFLYGVVPETTPAKKEVTEVSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="111" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LSD0</accession>
    <name>Q1LSD0_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Octanoyltransferase</fullName>
            <ecNumber evidence="6">2.3.1.181</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Lipoate-protein ligase B</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">Lipoyl/octanoyl transferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">Octanoyl-[acyl-carrier-protein]-protein N-octanoyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6 8" type="primary">lipB</name>
        <name evidence="8" type="ordered locus">Rmet_0060</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5 6">Catalyzes the transfer of endogenously produced octanoic acid from octanoyl-acyl-carrier-protein onto the lipoyl domains of lipoate-dependent enzymes. Lipoyl-ACP can also act as a substrate although octanoyl-ACP is likely to be the physiological substrate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 6">
            <text>L-lysyl-[protein] + octanoyl-[ACP] = H(+) + holo-[ACP] + N(6)-octanoyl-L-lysyl-[protein]</text>
            <dbReference type="Rhea" id="RHEA:17665"/>
            <dbReference type="Rhea" id="RHEA-COMP:9636"/>
            <dbReference type="Rhea" id="RHEA-COMP:9685"/>
            <dbReference type="Rhea" id="RHEA-COMP:9752"/>
            <dbReference type="Rhea" id="RHEA-COMP:9928"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:64479"/>
            <dbReference type="ChEBI" id="CHEBI:78463"/>
            <dbReference type="ChEBI" id="CHEBI:78809"/>
            <dbReference type="EC" id="2.3.1.181"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 6">Protein modification; protein lipoylation via endogenous pathway; protein N(6)-(lipoyl)lysine from octanoyl-[acyl-carrier-protein]: step 1/2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="miscellaneous">
        <text evidence="6">In the reaction, the free carboxyl group of octanoic acid is attached via an amide linkage to the epsilon-amino group of a specific lysine residue of lipoyl domains of lipoate-dependent enzymes.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the LipB family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.181" evidence="6"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF06946.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0060"/>
    <dbReference type="EnsemblBacteria" id="ABF06946">
        <property type="protein sequence ID" value="ABF06946"/>
        <property type="gene ID" value="Rmet_0060"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0060"/>
    <dbReference type="eggNOG" id="COG0321">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035168_3_1_4"/>
    <dbReference type="OMA" id="GEVTYHC"/>
    <dbReference type="UniPathway" id="UPA00538">
        <property type="reaction ID" value="UER00592"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016874">
        <property type="term" value="F:ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033819">
        <property type="term" value="F:lipoyl(octanoyl) transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009249">
        <property type="term" value="P:protein lipoylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd16444">
        <property type="entry name" value="LipB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00013">
        <property type="entry name" value="LipB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004143">
        <property type="entry name" value="BPL_LPL_catalytic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000544">
        <property type="entry name" value="Octanoyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020605">
        <property type="entry name" value="Octanoyltransferase_CS"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="SSF55681"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00214">
        <property type="entry name" value="lipB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51733">
        <property type="entry name" value="BPL_LPL_CATALYTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01313">
        <property type="entry name" value="LIPB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6 8" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0436">Ligase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6 8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="BPL/LPL catalytic" evidence="7">
        <location>
            <begin position="52"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="6">
        <location>
            <begin position="92"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="6">
        <location>
            <begin position="164"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="6">
        <location>
            <begin position="177"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="active site" description="Acyl-thioester intermediate" evidence="6">
        <location>
            <position position="195"/>
        </location>
    </feature>
    <feature type="site" description="Lowers pKa of active site Cys" evidence="6">
        <location>
            <position position="161"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00024732"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ABF06946.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="264" mass="28335" checksum="88912DB96C3D7892" modified="2006-05-30" version="1">MTKQIGPARALRRPALFVTLLPMHPIHVIDRGRQPYQPCFDAMRDFTATRTPETPDQIWLVEHPPVYTLGQAGDPAHLLAPDDTIPVVQIDRGGQITYHGPGQVVAYLLLDLRRRHLMVRELVHDIEQAVLDTLAAYNLAAERKAGAPGIYLSDGKHQGAKIAALGLKIRNGCSYHGVSLNVQMDLSPFLRINPCGYAGLETVDMATAGAAFTAADDAGGAVHRPVTATQQPEIAQRLAAALCEVLAAREARAPVPGQAAAEAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-01-04" modified="2021-06-02" version="70" xmlns="http://uniprot.org/uniprot">
    <accession>Q5NUV6</accession>
    <name>Q5NUV6_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_6228</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="1 2">
        <name>pMOL28</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid pMOL28</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000355">
        <property type="protein sequence ID" value="ABF13087.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011239954.1">
        <property type="nucleotide sequence ID" value="NC_007972.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_161693.1">
        <property type="nucleotide sequence ID" value="NC_006525.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF13087">
        <property type="protein sequence ID" value="ABF13087"/>
        <property type="gene ID" value="Rmet_6228"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6228"/>
    <dbReference type="HOGENOM" id="CLU_539344_0_0_4"/>
    <dbReference type="OMA" id="NGRHEVH"/>
    <dbReference type="OrthoDB" id="961967at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid pMOL28"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF13087.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="516" mass="57745" checksum="1E8646855AD46F06" modified="2005-01-04" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2005-01-04" modified="2022-01-19" version="69" xmlns="http://uniprot.org/uniprot">
    <accession>Q5NUV9</accession>
    <name>Q5NUV9_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_6231</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
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            <taxon>Bacteria</taxon>
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            <taxon>Betaproteobacteria</taxon>
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            <taxon>Cupriavidus</taxon>
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        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
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                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid pMOL28</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000355">
        <property type="protein sequence ID" value="ABF13090.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011239951.1">
        <property type="nucleotide sequence ID" value="NC_006525.1"/>
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    <dbReference type="RefSeq" id="YP_161690.1">
        <property type="nucleotide sequence ID" value="NC_006525.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF13090">
        <property type="protein sequence ID" value="ABF13090"/>
        <property type="gene ID" value="Rmet_6231"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6231"/>
    <dbReference type="HOGENOM" id="CLU_142250_0_0_4"/>
    <dbReference type="OMA" id="HEDGYRI"/>
    <dbReference type="OrthoDB" id="2062414at2"/>
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        <property type="component" value="Plasmid pMOL28"/>
    </dbReference>
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        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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    <keyword evidence="1" id="KW-0472">Membrane</keyword>
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    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
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        <location>
            <begin position="104"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="128"/>
            <end position="152"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF13090.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="156" mass="16404" checksum="2E7F74E68D42E684" modified="2005-01-04" version="1">MSRLIKVTFNSTSGEETTAFATLHTDDTVELPKRMAARVTAAVDAGEGYALVAHQRGSSVPLVHMVDAVYRLDTEHATGDGWLSRLADALTNPTKDQMQAYGRYYHTLSAACSVGFAGYVAGVQSINATVVINAACLLLGAAVLFALGAVLAKGEK</sequence>
</entry>
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    <accession>D3DXN0</accession>
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            <fullName evidence="1">Uncharacterized protein</fullName>
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    <organism evidence="1 2">
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        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
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                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
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            <dbReference type="PubMed" id="20463976"/>
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        </source>
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    <dbReference type="EnsemblBacteria" id="ADC45050">
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        <property type="gene ID" value="Rmet_6433"/>
    </dbReference>
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        <property type="component" value="Chromosome"/>
    </dbReference>
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    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADC45050.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="59" mass="6037" checksum="893F7C858470F0DE" modified="2010-03-23" version="1">MGAGAGPNVPPKSCDTSKMSAMLAKNTWTLPLLYASADIIAINQSPAVVPPPHGSCNLM</sequence>
</entry>
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    <accession>D3DXT8</accession>
    <name>D3DXT8_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name evidence="2" type="ordered locus">Rmet_6500</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ADC45108.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="EnsemblBacteria" id="ADC45108">
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        <property type="gene ID" value="Rmet_6500"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6500"/>
    <dbReference type="HOGENOM" id="CLU_3358004_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
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    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="15"/>
            <end position="36"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADC45108.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="36" mass="3931" checksum="7E69CF2CD5A086C3" modified="2010-03-23" version="1">MPVESGFGTDCKEVGTKYQTEEGTRAEGKEASQNHE</sequence>
</entry>
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    <accession>D3DY09</accession>
    <name>D3DY09_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_6577</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
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    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ADC45179.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADC45179">
        <property type="protein sequence ID" value="ADC45179"/>
        <property type="gene ID" value="Rmet_6577"/>
    </dbReference>
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    <dbReference type="HOGENOM" id="CLU_3410023_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADC45179.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="29" mass="3452" checksum="408EA14494D377A4" modified="2010-03-23" version="1">MFRHVTVAQRPRPNIPNREKLFGSDCIRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-03-23" modified="2021-06-02" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>D3DY13</accession>
    <name>D3DY13_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_6582</name>
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    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
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        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
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            <taxon>Burkholderiaceae</taxon>
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        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ADC45183.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_6582"/>
    <dbReference type="EnsemblBacteria" id="ADC45183">
        <property type="protein sequence ID" value="ADC45183"/>
        <property type="gene ID" value="Rmet_6582"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6582"/>
    <dbReference type="HOGENOM" id="CLU_2790984_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="54"/>
            <end position="68"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADC45183.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="68" mass="7408" checksum="754E137000C75671" modified="2010-03-23" version="1">MRWWHGHGPSFRNRRTPSIDPSPAGGEGGQHRAIPTLSVRAPFSQAGEGLGERAKASYADDLTPKRAR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1998-12-15" modified="2022-05-25" version="111" xmlns="http://uniprot.org/uniprot">
    <accession>P94139</accession>
    <accession>Q1LAI3</accession>
    <accession>Q44008</accession>
    <name>CZCN_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName>Cobalt-zinc-cadmium resistance protein CzcN</fullName>
        </recommendedName>
        <alternativeName>
            <fullName>Cation efflux system protein CzcN</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">czcN</name>
        <name type="ordered locus">Rmet_5984</name>
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    <organism>
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        <name type="synonym">Ralstonia metallidurans</name>
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            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
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            <taxon>Burkholderiaceae</taxon>
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    <geneLocation type="plasmid">
        <name>pMOL30</name>
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    <reference key="1">
        <citation type="journal article" date="1992" name="J. Bacteriol." volume="174" first="8102" last="8110">
            <title>CzcR and CzcD, gene products affecting regulation of resistance to cobalt, zinc, and cadmium (czc system) in Alcaligenes eutrophus.</title>
            <authorList>
                <person name="Nies D.H."/>
            </authorList>
            <dbReference type="PubMed" id="1459958"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC DNA]</scope>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <reference key="3">
        <citation type="journal article" date="1997" name="Mol. Microbiol." volume="23" first="493" last="503">
            <title>Two-component regulatory system involved in transcriptional control of heavy-metal homoeostasis in Alcaligenes eutrophus.</title>
            <authorList>
                <person name="van der Lelie D."/>
                <person name="Schwuchow T."/>
                <person name="Schwidetzky T."/>
                <person name="Wuertz S."/>
                <person name="Baeyens W."/>
                <person name="Mergeay M."/>
                <person name="Nies D.H."/>
            </authorList>
            <dbReference type="PubMed" id="9044283"/>
            <dbReference type="DOI" id="10.1046/j.1365-2958.1997.d01-1866.x"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 50-216</scope>
    </reference>
    <reference key="4">
        <citation type="journal article" date="1995" name="J. Ind. Microbiol." volume="14" first="142" last="153">
            <title>The czc operon of Alcaligenes eutrophus CH34: from resistance mechanism to the removal of heavy metals.</title>
            <authorList>
                <person name="Diels L."/>
                <person name="Dong Q."/>
                <person name="van der Lelie D."/>
                <person name="Baeyens W."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="7766206"/>
            <dbReference type="DOI" id="10.1007/BF01569896"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 171-216</scope>
        <scope>IDENTIFICATION</scope>
        <scope>DISCUSSION OF FUNCTION</scope>
    </reference>
    <comment type="function">
        <text>Component of the CZC cation-efflux system that confers resistance to cobalt, zinc and cadmium.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell inner membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">To A.xylosoxydans NccN.</text>
    </comment>
    <comment type="sequence caution" evidence="2">
        <conflict type="erroneous initiation">
            <sequence resource="EMBL-CDS" id="ABF12843" version="1"/>
        </conflict>
    </comment>
    <dbReference type="EMBL" id="X71400">
        <property type="protein sequence ID" value="CAA50523.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP000354">
        <property type="protein sequence ID" value="ABF12843.1"/>
        <property type="status" value="ALT_INIT"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="X98451">
        <property type="protein sequence ID" value="CAA67079.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011229344.1">
        <property type="nucleotide sequence ID" value="NC_007971.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_145591.1">
        <property type="nucleotide sequence ID" value="NC_006466.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="P94139"/>
    <dbReference type="SMR" id="P94139"/>
    <dbReference type="EnsemblBacteria" id="ABF12843">
        <property type="protein sequence ID" value="ABF12843"/>
        <property type="gene ID" value="Rmet_5984"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5984"/>
    <dbReference type="HOGENOM" id="CLU_1018913_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid pMOL30"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046686">
        <property type="term" value="P:response to cadmium ion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007318">
        <property type="entry name" value="Phopholipid_MeTrfase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04191">
        <property type="entry name" value="PEMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-0105">Cadmium resistance</keyword>
    <keyword id="KW-0997">Cell inner membrane</keyword>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0170">Cobalt</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-0614">Plasmid</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <keyword id="KW-0813">Transport</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <feature type="chain" id="PRO_0000079763" description="Cobalt-zinc-cadmium resistance protein CzcN">
        <location>
            <begin position="1"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="27"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="50"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="116"/>
            <end position="136"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="216" mass="23753" checksum="F2429D4145AF2A53" modified="2005-05-10" version="2">MTDSSISPSHPAGLSRALDNFLTRHRIGVWRVVVTFVLASLLFGHSRWDGTWVSPLLLTLGMLGVSLATVGRLWCALYISGRKNNTLVTSGPYSLCRHPLYVCNLLGILGLGAMTESLAVTAVLALAFALMYPAVIRTEDRFLASAFPEFAEYARRTPAFFPRLSLYRGESTWTVHVSSFQRNIADSVWFLGLSVVVESFDLFHDAGVLRAVVTLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>Q1L9J7</accession>
    <name>Q1L9J7_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Tyrosine-based site-specific recombinase activity site-specific DNA integration</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">int</name>
        <name evidence="3" type="ordered locus">Rmet_6320</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3 4">
        <name>pMOL28</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid pMOL28</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000355">
        <property type="protein sequence ID" value="ABF13179.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008649488.1">
        <property type="nucleotide sequence ID" value="NC_007972.2"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF13179">
        <property type="protein sequence ID" value="ABF13179"/>
        <property type="gene ID" value="Rmet_6320"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6320"/>
    <dbReference type="HOGENOM" id="CLU_634414_0_0_4"/>
    <dbReference type="OMA" id="REDAHAY"/>
    <dbReference type="OrthoDB" id="745068at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid pMOL28"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.443.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011010">
        <property type="entry name" value="DNA_brk_join_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013762">
        <property type="entry name" value="Integrase-like_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002104">
        <property type="entry name" value="Integrase_catalytic"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00589">
        <property type="entry name" value="Phage_integrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56349">
        <property type="entry name" value="SSF56349"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51898">
        <property type="entry name" value="TYR_RECOMBINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tyr recombinase" evidence="2">
        <location>
            <begin position="162"/>
            <end position="392"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF13179.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="398" mass="43869" checksum="E54E9D23ED3D204E" modified="2006-05-30" version="1">MEDLLPTLPSLDHRDALPNALASPVLPAGQSDAQLVRTWLDTKYAAGLGIRASTRAQYALEAHRLLWYAHALGRPLASWQVADANGYLAFLADPPAHAVGDGRVRQDSPAWRPLRGPLSEASRRQTATIVGGLFEWLVGVEALRVNPFQAVPRARSARGPGSQRRFLEAEQLAAVFDAVEARPAPTLWAQLHKARDRLLLALLFHTGLRASEVTALTWPDFERQRGRHGDFWTVRIREAKGGSDQLVPCDNVMAELARFRQLVGLSPEPRATDTMAVVPAMAGGRQRLAPLTDTLALQRRLSRPVRTRQGLYTIVRQVFEAAAVRLEASGYAEGAANLRAASTHWLRHSHATHLLRAGVPVTDVQRTLRHRDINTTRRYTHEALEDVARSIGGKLPAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="61" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LAC4</accession>
    <name>Q1LAC4_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative metalloprotease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_6043</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2 3">
        <name>pMOL30</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid pMOL30</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000354">
        <property type="protein sequence ID" value="ABF12902.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF12902">
        <property type="protein sequence ID" value="ABF12902"/>
        <property type="gene ID" value="Rmet_6043"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6043"/>
    <dbReference type="HOGENOM" id="CLU_694205_0_0_4"/>
    <dbReference type="OMA" id="NHYAFET"/>
    <dbReference type="OrthoDB" id="2100014at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid pMOL30"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004176">
        <property type="term" value="F:ATP-dependent peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.760">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037219">
        <property type="entry name" value="Peptidase_M41-like"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140990">
        <property type="entry name" value="SSF140990"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="64"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="96"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="130"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="160"/>
            <end position="184"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF12902.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="408" mass="44919" checksum="2B822E355B464F86" modified="2006-05-30" version="1">MDFAERAARGEEGARTGARMRRHSLTLTLAKILFKDGPVKFFQLVYILGRSKLRRTYDSHTRRFLQAGHAALLAAGVIVLWLAVRDPSLIDARSTAGRIFCGAIGMAFTLSGATWFLMPAFQRATLLGEVTAGTLYGMLLAGVTWLGWERAAALYQANPVALVAGVLVVAALVLAFAHPLSLFLRFRLLRLRTNHYAFETPYHQMLASRPARGQYRGVAIHEAGHALMYSLGDRVPEDAIAYIDFDTISPNLGQVSLPTLKEGDISMAMLEWKMHTYLAGMVAEVLRAGTHGMGVSGDLEGWSELVGPYLLLRTDVVYFANPSTELEIESNKREVAALKKHMLAVLTRFMRANWHVVERIADCLEEVDCLDHQQLAGLISGVVRTGGLPTPVWPDHMDGTPLRQIPGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LAE2</accession>
    <name>Q1LAE2_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_6025</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="1 2">
        <name>pMOL30</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid pMOL30</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000354">
        <property type="protein sequence ID" value="ABF12884.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011514800.1">
        <property type="nucleotide sequence ID" value="NC_007971.2"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF12884">
        <property type="protein sequence ID" value="ABF12884"/>
        <property type="gene ID" value="Rmet_6025"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6025"/>
    <dbReference type="HOGENOM" id="CLU_1748148_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid pMOL30"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF12884.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="149" mass="16787" checksum="E986EF328DCC222A" modified="2006-05-30" version="1">MSSQIVNSARAVIGASGTIDGSEAPTFAVFDIDRAFIATVSRLINLCNEHKLTEARTVHYPAWGPGWIEEELKLQNGELVVQPNGIFRFTDYPKYGGYLIQTADVDFNQLRSKFDSAVDGEVLFLAKEPYVRQYYEQEYEQSARELVPS</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-12-12" modified="2022-08-03" version="95" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LAN7</accession>
    <name>EFG2_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Elongation factor G 2</fullName>
            <shortName evidence="1">EF-G 2</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">fusA2</name>
        <name type="ordered locus">Rmet_5930</name>
    </gene>
    <organism>
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. EF-G/EF-2 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12789.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008645473.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q1LAN7"/>
    <dbReference type="SMR" id="Q1LAN7"/>
    <dbReference type="STRING" id="266264.Rmet_5930"/>
    <dbReference type="PRIDE" id="Q1LAN7"/>
    <dbReference type="EnsemblBacteria" id="ABF12789">
        <property type="protein sequence ID" value="ABF12789"/>
        <property type="gene ID" value="Rmet_5930"/>
    </dbReference>
    <dbReference type="GeneID" id="60823727"/>
    <dbReference type="KEGG" id="rme:Rmet_5930"/>
    <dbReference type="eggNOG" id="COG0480">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002794_4_1_4"/>
    <dbReference type="OMA" id="FRVVQMM"/>
    <dbReference type="OrthoDB" id="88967at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd16262">
        <property type="entry name" value="EFG_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01434">
        <property type="entry name" value="EFG_mtEFG1_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03713">
        <property type="entry name" value="EFG_mtEFG_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00054_B">
        <property type="entry name" value="EF_G_EF_2_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041095">
        <property type="entry name" value="EFG_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009022">
        <property type="entry name" value="EFG_III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035647">
        <property type="entry name" value="EFG_III/V"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035649">
        <property type="entry name" value="EFG_V"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000640">
        <property type="entry name" value="EFG_V-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004161">
        <property type="entry name" value="EFTu-like_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031157">
        <property type="entry name" value="G_TR_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000795">
        <property type="entry name" value="T_Tr_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004540">
        <property type="entry name" value="Transl_elong_EFG/EF2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005517">
        <property type="entry name" value="Transl_elong_EFG/EF2_IV"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00679">
        <property type="entry name" value="EFG_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14492">
        <property type="entry name" value="EFG_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03764">
        <property type="entry name" value="EFG_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00009">
        <property type="entry name" value="GTP_EFTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03144">
        <property type="entry name" value="GTP_EFTU_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00315">
        <property type="entry name" value="ELONGATNFCT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00838">
        <property type="entry name" value="EFG_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00889">
        <property type="entry name" value="EFG_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="SSF50447"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="SSF54211"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54980">
        <property type="entry name" value="SSF54980"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00484">
        <property type="entry name" value="EF-G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00301">
        <property type="entry name" value="G_TR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51722">
        <property type="entry name" value="G_TR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0251">Elongation factor</keyword>
    <keyword id="KW-0342">GTP-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0614">Plasmid</keyword>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000263488" description="Elongation factor G 2">
        <location>
            <begin position="1"/>
            <end position="702"/>
        </location>
    </feature>
    <feature type="domain" description="tr-type G">
        <location>
            <begin position="8"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="17"/>
            <end position="24"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="88"/>
            <end position="92"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="142"/>
            <end position="145"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00054"/>
        </source>
    </evidence>
    <sequence length="702" mass="77270" checksum="E8D767FF8C6FFDB8" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="58" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LAQ8</accession>
    <name>Q1LAQ8_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_5909</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12768.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518116.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5909"/>
    <dbReference type="EnsemblBacteria" id="ABF12768">
        <property type="protein sequence ID" value="ABF12768"/>
        <property type="gene ID" value="Rmet_5909"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5909"/>
    <dbReference type="HOGENOM" id="CLU_1438901_0_0_4"/>
    <dbReference type="OMA" id="NATRVWH"/>
    <dbReference type="OrthoDB" id="1535047at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF12768.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="191" mass="20585" checksum="1411DB2D3BE5A95E" modified="2006-05-30" version="1">MSRFQVTASQGPVTAGQQSKVSQDDLHAVHRLRAVVATYRTTLQAYTLVVSPDELMSLSRGPEPSHSARDYRAQAHLDRLLNSVRDSGLALPSAIVVAVSGGRMQCECDHLYTLEMHPRDGDHLIVLDGYDRLAALASTGRGNIKTLVTGVLCCTAIDGAAVESQSANEADRAMMHTSAAARWAGAMRVWQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LAW7</accession>
    <name>Q1LAW7_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Protein-tyrosine-phosphatase</fullName>
            <ecNumber evidence="3">3.1.3.48</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">epsP</name>
        <name evidence="7" type="ordered locus">Rmet_5850</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="8">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="8">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>H2O + O-phospho-L-tyrosyl-[protein] = L-tyrosyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:10684"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:10137"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:82620"/>
            <dbReference type="EC" id="3.1.3.48"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the low molecular weight phosphotyrosine protein phosphatase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.48" evidence="3"/>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12709.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5850"/>
    <dbReference type="EnsemblBacteria" id="ABF12709">
        <property type="protein sequence ID" value="ABF12709"/>
        <property type="gene ID" value="Rmet_5850"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5850"/>
    <dbReference type="eggNOG" id="COG0394">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_071415_1_1_4"/>
    <dbReference type="OMA" id="VDPYQRH"/>
    <dbReference type="OrthoDB" id="2062716at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004725">
        <property type="term" value="F:protein tyrosine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006470">
        <property type="term" value="P:protein dephosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023485">
        <property type="entry name" value="Ptyr_pPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036196">
        <property type="entry name" value="Ptyr_pPase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017867">
        <property type="entry name" value="Tyr_phospatase_low_mol_wt"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01451">
        <property type="entry name" value="LMWPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00719">
        <property type="entry name" value="LMWPTPASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00226">
        <property type="entry name" value="LMWPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52788">
        <property type="entry name" value="SSF52788"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0614">Plasmid</keyword>
    <keyword evidence="5" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="LMWPc" evidence="6">
        <location>
            <begin position="14"/>
            <end position="151"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABF12709.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="164" mass="17507" checksum="4B089AF1EE663584" modified="2006-05-30" version="1">MEVMREGKEAAFIRSILVVCIGNRCRSPMAAALLGRALPQCRVLSAGLAPPVGAAADPRAVRLLAVEGSDLTDHRARAVSAALVAGADLVLVMDEEQRDALEDMYPQARGKTYRLCEQEQTDVPDPFSGSMSMFVIALGLIKMGVESWSARIHAAEPAHRHGGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="99" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LB23</accession>
    <name>Q1LB23_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Transcriptional regulator, GntR family (HTH:Aminotransferase)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_5794</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">In the C-terminal section; belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12653.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011520190.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5794"/>
    <dbReference type="EnsemblBacteria" id="ABF12653">
        <property type="protein sequence ID" value="ABF12653"/>
        <property type="gene ID" value="Rmet_5794"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5794"/>
    <dbReference type="eggNOG" id="COG1167">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017584_0_0_4"/>
    <dbReference type="OMA" id="LLMTYQL"/>
    <dbReference type="OrthoDB" id="1024978at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008483">
        <property type="term" value="F:transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009058">
        <property type="term" value="P:biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000524">
        <property type="entry name" value="Tscrpt_reg_HTH_GntR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00392">
        <property type="entry name" value="GntR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00345">
        <property type="entry name" value="HTH_GNTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="SSF53383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50949">
        <property type="entry name" value="HTH_GNTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="HTH gntR-type" evidence="2">
        <location>
            <begin position="23"/>
            <end position="91"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF12653.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="465" mass="49551" checksum="994E472A727A663C" modified="2006-05-30" version="1">MRTKTSALTGKPAWIGEFSPTGGPRYLQIAGFLEHAVADGRLSPGDRLAPQRELAAMLGVDLTTVTRALAEARRRRLVEPRGSLGTFVAAPVFELAQTVDLSMNVPPPPESIDFDDMLRRGLAQVTLHADASLLMTYQLGGGTQPDREAGARWLGPMLGTVASSRVIASPGAQSALSAVILSLSDPRSVIAAEPLIYPGLRAAAAQTGRRVVTVATDDDGMRPDALEAACRAHAIRLIYLNPTLQNPTTHTMPEHRRRDIARVAARCGARIIEDDPYWLLTPDAPPPVAALAPAQTIHIATLSKCLSPGLRTAYVVAPDVDTQELILAALRAITLMASPIATALATQWIRDGSAEALLNGIRSEAAARQQMARQWLIGADQSPQSGIHVWHPLPSYWTSSGFSRAASDEHLRVTASDAFSDGADAPNAIRISLGGVAERAQLSMALKKLSGLLARKPPHAMLQVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LBJ5</accession>
    <name>Q1LBJ5_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Probable membrane transporter protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Rmet_5622</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="7">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="7">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cell membrane</location>
            <topology evidence="5">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the 4-toluene sulfonate uptake permease (TSUP) (TC 2.A.102) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12481.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011520028.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5622"/>
    <dbReference type="EnsemblBacteria" id="ABF12481">
        <property type="protein sequence ID" value="ABF12481"/>
        <property type="gene ID" value="Rmet_5622"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5622"/>
    <dbReference type="eggNOG" id="COG0730">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_054750_5_6_4"/>
    <dbReference type="OMA" id="MSFWAWG"/>
    <dbReference type="OrthoDB" id="1274094at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002781">
        <property type="entry name" value="TM_pro_TauE-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01925">
        <property type="entry name" value="TauE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0614">Plasmid</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="7"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="44"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="71"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="97"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="129"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="170"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="198"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="224"/>
            <end position="245"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU363041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABF12481.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="248" mass="26294" checksum="6389B212D47DBD3D" modified="2006-05-30" version="1">MPVDSVFLIVALGAIVAGFVQGLSGFAFGMVAMSFWAWGVEPRLASAMSVFGALTGQLLAAFTVRRKFHLATVLPFLLGGIVGIPIGVSILPRLDVHLFKLCLGIVLVIWCPIMLFSNRIPPIRRGGRLADAVVGAIGGIMGGIGGFTGIAPTLWCTLRGFDKDKQRVVIQNFNLGALLVTMAVYVGTGIITRDMLPMFAIVAPAMLIPTLLGTRVYIGISDIAFRRIVLTLLTCSGIAMLVAAIPKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="92" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LBR9</accession>
    <name>Q1LBR9_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Putative Phthalate 4,5-dioxygenase, subunit alpha</fullName>
            <ecNumber evidence="8">1.14.12.7</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Rmet_5548</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="9">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="9">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="journal article" date="2021" name="J. Biol. Chem." volume="297" first="101416" last="101416">
            <title>Molecular insights into substrate recognition and catalysis by phthalate dioxygenase from Comamonas testosteroni.</title>
            <authorList>
                <person name="Mahto J.K."/>
                <person name="Neetu N."/>
                <person name="Waghmode B."/>
                <person name="Kuatsjah E."/>
                <person name="Sharma M."/>
                <person name="Sircar D."/>
                <person name="Sharma A.K."/>
                <person name="Tomar S."/>
                <person name="Eltis L.D."/>
                <person name="Kumar P."/>
            </authorList>
            <dbReference type="PubMed" id="34800435"/>
            <dbReference type="DOI" id="10.1016/j.jbc.2021.101416"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (1.84 ANGSTROMS)</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the bacterial ring-hydroxylating dioxygenase alpha subunit family.</text>
    </comment>
    <dbReference type="EC" id="1.14.12.7" evidence="8"/>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12407.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519954.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="PDB" id="7FHR">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="1.84 A"/>
        <property type="chains" value="A=1-439"/>
    </dbReference>
    <dbReference type="SMR" id="Q1LBR9"/>
    <dbReference type="STRING" id="266264.Rmet_5548"/>
    <dbReference type="EnsemblBacteria" id="ABF12407">
        <property type="protein sequence ID" value="ABF12407"/>
        <property type="gene ID" value="Rmet_5548"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5548"/>
    <dbReference type="eggNOG" id="COG4638">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039484_2_1_4"/>
    <dbReference type="OMA" id="QWHVPVD"/>
    <dbReference type="OrthoDB" id="691776at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018620">
        <property type="term" value="F:phthalate 4,5-dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019439">
        <property type="term" value="P:aromatic compound catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.102.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045623">
        <property type="entry name" value="LigXa_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017941">
        <property type="entry name" value="Rieske_2Fe-2S"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036922">
        <property type="entry name" value="Rieske_2Fe-2S_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015881">
        <property type="entry name" value="Ring-hydroxy_dOase_2Fe2S_BS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19301">
        <property type="entry name" value="LigXa_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00355">
        <property type="entry name" value="Rieske"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50022">
        <property type="entry name" value="SSF50022"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51296">
        <property type="entry name" value="RIESKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00570">
        <property type="entry name" value="RING_HYDROXYL_ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword evidence="10" id="KW-0002">3D-structure</keyword>
    <keyword evidence="3" id="KW-0058">Aromatic hydrocarbons catabolism</keyword>
    <keyword evidence="8" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="5" id="KW-0408">Iron</keyword>
    <keyword evidence="6" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-0614">Plasmid</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rieske" evidence="7">
        <location>
            <begin position="27"/>
            <end position="133"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008751"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022797"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ABF12407.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="10">
        <source>
            <dbReference type="PDB" id="7FHR"/>
        </source>
    </evidence>
    <sequence length="439" mass="49468" checksum="BC22B39714962C75" modified="2006-05-30" version="1">MLSREDNELLVRVGPGTAMGTMMRLYWIPFLKSSEVTAGGQPYRVRLLGEDLVAFRDNSGNVGLVDHTCPHRGAPMVFGRNENDGLRCVYHGWKFKVSGQCEEMPCEPADSPMCKRMKIKAYPVKERNGILWAYMGPDAENAPELPDVEWNMVPEEQVAISMRVQECNWLQALEGELDSAHAAILHGRVGEGGVINQWRQAQDLSPTFECVQHDAGISIGARRKTPDGENYVRVNQFLMPFWTLVPPQSQFPELSGHAWVPIDDEHTLCLMFSYHPAKPFYERTRKLFKEGHNGRETGHHSDNAFEKRPVTEPYHTYWSKFNRGNAYQFDYQSQVEKYNSGMPGLWIQDAACQSGTTPILDRSKEHLGTSDTGVARMRRVLLEAVKKLVATGEHPVSSNAPAAFRWRAVSLTIPLGGDWTKLGEEAMRAEPGKDFGYTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LC48</accession>
    <name>Q1LC48_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Peptidase, M20D subfamily</fullName>
            <ecNumber evidence="3">3.5.1.32</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_5419</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EC" id="3.5.1.32" evidence="3"/>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12278.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519826.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5419"/>
    <dbReference type="EnsemblBacteria" id="ABF12278">
        <property type="protein sequence ID" value="ABF12278"/>
        <property type="gene ID" value="Rmet_5419"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5419"/>
    <dbReference type="eggNOG" id="COG1473">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023257_1_1_4"/>
    <dbReference type="OMA" id="MKMHACG"/>
    <dbReference type="OrthoDB" id="796259at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047980">
        <property type="term" value="F:hippurate hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017439">
        <property type="entry name" value="Amidohydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036264">
        <property type="entry name" value="Bact_exopeptidase_dim_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002933">
        <property type="entry name" value="Peptidase_M20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011650">
        <property type="entry name" value="Peptidase_M20_dimer"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11014">
        <property type="entry name" value="PTHR11014"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07687">
        <property type="entry name" value="M20_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01546">
        <property type="entry name" value="Peptidase_M20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005962">
        <property type="entry name" value="Pept_M20D_amidohydro"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55031">
        <property type="entry name" value="SSF55031"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01891">
        <property type="entry name" value="amidohydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="M20_dimer" evidence="2">
        <location>
            <begin position="188"/>
            <end position="284"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF12278.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="393" mass="41345" checksum="B8243399DAD0358E" modified="2006-05-30" version="1">MTTTPIPAAISSAASRFADLRHRIHAHPELGADVPRTAAMVAGLLREWGYDVHTGIGGHGIVAQLNVGNGPRRIGIRADMDALPLQERTGLPYASEVPGRMHACGHDGHTATLLAAAEYLAATRNFDGTLNLIFQPDEEGMTGALSMMEDGLFERFPCDAIYAFHNAPGLPVGMAVVQSGAQGGSSDRVTVTFRGKGGHGAIPNRAADPTLALAHTVVALQGIIARNVAPVDAGVISVGQIEAGTTYNIIPETATLKLSVRATLPEVRKLLEERIRAVVTHQAASAGVEAEIEYLHLVPAIRNTPAEAAIAREAVASVLGNDNVIQVPPSTQMGSEDFAWMLEKVPGCYFVLGNGTGEWVGCSVHNDGYDFNDALIPLGAACWVALAEGYLKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="61" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LC64</accession>
    <name>Q1LC64_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_5403</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12262.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519810.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF12262">
        <property type="protein sequence ID" value="ABF12262"/>
        <property type="gene ID" value="Rmet_5403"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5403"/>
    <dbReference type="eggNOG" id="COG4320">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032121_0_0_4"/>
    <dbReference type="OMA" id="DFDEAHP"/>
    <dbReference type="OrthoDB" id="571864at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018721">
        <property type="entry name" value="DUF2252"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39441">
        <property type="entry name" value="PTHR39441"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10009">
        <property type="entry name" value="DUF2252"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="15"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="15"/>
            <end position="29"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF12262.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="470" mass="51898" checksum="85FF8B7665DF33E8" modified="2006-05-30" version="1">MADIRTLAERQAIGREARSRAKRSSNAEIGNTDRDPVALLEQNSAGRVEALVPLRYGRMSVSPFTFFRGSAILQAHDLAATANAGIAFPICGDAHLMNFGGFATPERQLVFDLNDFDEVAVGPWEWDVKRLAGSLAIAGEHMGIARDTVSDIVATAVHEYRDRMEEYAGYSALDLWNEIVSFERMLEAATSEEGRRTILKAKEKAAGRTNESMLNKMAAQRDGQWWIQDAPPAIFHPSGPTSLLGEHDQWSNTEAWRGKLARAFDGYLKTLPSERRALIDHFSLQDVAFKVVGVGSVGTFCLVLLMVDSHEQPLFLQVKEARDSVIALHYDAEGPAHQGQRVVSGQRLLQAASDAFLGWTSGPANRQFYFRQLRDMKVSADVERMSNGVLQGYARFCGWALARAHAKASGKAVEIAAYLGSGERFADAITEYSFACATQNLKDYEAFKLACRTGKIEARSDEDMAADFRM</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LCR3</accession>
    <name>Q1LCR3_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Putative NADH dehydrogenase/NAD(P)H nitroreductase Rmet_5204</fullName>
            <ecNumber evidence="5">1.-.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Rmet_5204</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="8">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="8">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the nitroreductase family. HadB/RutE subfamily.</text>
    </comment>
    <dbReference type="EC" id="1.-.-.-" evidence="5"/>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12063.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519609.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5204"/>
    <dbReference type="EnsemblBacteria" id="ABF12063">
        <property type="protein sequence ID" value="ABF12063"/>
        <property type="gene ID" value="Rmet_5204"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5204"/>
    <dbReference type="eggNOG" id="COG0778">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_084441_0_0_4"/>
    <dbReference type="OMA" id="THNGWLD"/>
    <dbReference type="OrthoDB" id="1847197at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02148">
        <property type="entry name" value="RutE-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.109.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01204">
        <property type="entry name" value="Oxidoreductase_RutE_HadB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029479">
        <property type="entry name" value="Nitroreductase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000415">
        <property type="entry name" value="Nitroreductase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023936">
        <property type="entry name" value="RutE-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00881">
        <property type="entry name" value="Nitroreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55469">
        <property type="entry name" value="SSF55469"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 5" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="2 5" id="KW-0288">FMN</keyword>
    <keyword evidence="5" id="KW-0520">NAD</keyword>
    <keyword evidence="3 5" id="KW-0521">NADP</keyword>
    <keyword evidence="4 5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-0614">Plasmid</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nitroreductase" evidence="6">
        <location>
            <begin position="18"/>
            <end position="175"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABF12063.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="197" mass="21195" checksum="159B8D19F356C2DE" modified="2006-05-30" version="1">MSAPVASDVLDTLFLTARSHNGWLDGDVSDATLTRLYALVSQGPTSANCSPMRLVFVRSDAARARLLPTLAPANVDKVRHAPVTAIVAYDTAFHAFGETLFPHRPAMFDTFAADPALAAETAMRNGSMQGGYLILAARALGLDCGPLSGFNADAVNRAFFPDGRWRVNFLCNLGKGDPARLFAKLPRLPFDQACRIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-10-07" version="53" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LDB7</accession>
    <name>Q1LDB7_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_4997</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11859.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4997"/>
    <dbReference type="EnsemblBacteria" id="ABF11859">
        <property type="protein sequence ID" value="ABF11859"/>
        <property type="gene ID" value="Rmet_4997"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4997"/>
    <dbReference type="HOGENOM" id="CLU_155885_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF11859.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="103" mass="11196" checksum="3987A490C1876250" modified="2006-05-30" version="1">MNLASIIADLESADSTLTIVAKRPWTANSDARLVSLTDEYSIPGNVLLEGFEYFLEVSIALDEVLGESASRLSSDQRVAAIVYYAENDDYPDWLNAIAGSLHG</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LDQ3</accession>
    <name>Q1LDQ3_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative CDP-alcohol phosphatidyltransferase</fullName>
            <ecNumber evidence="3">2.7.8.8</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_4861</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CDP-alcohol phosphatidyltransferase class-I family.</text>
    </comment>
    <dbReference type="EC" id="2.7.8.8" evidence="3"/>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11723.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519284.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4861"/>
    <dbReference type="DNASU" id="4041723"/>
    <dbReference type="EnsemblBacteria" id="ABF11723">
        <property type="protein sequence ID" value="ABF11723"/>
        <property type="gene ID" value="Rmet_4861"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4861"/>
    <dbReference type="eggNOG" id="COG1183">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_049944_5_2_4"/>
    <dbReference type="OMA" id="CGMISKS"/>
    <dbReference type="OrthoDB" id="1232102at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003882">
        <property type="term" value="F:CDP-diacylglycerol-serine O-phosphatidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008654">
        <property type="term" value="P:phospholipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1760">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000462">
        <property type="entry name" value="CDP-OH_P_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043130">
        <property type="entry name" value="CDP-OH_PTrfase_TM_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01066">
        <property type="entry name" value="CDP-OH_P_transf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00379">
        <property type="entry name" value="CDP_ALCOHOL_P_TRANSF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 3" id="KW-0808">Transferase</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="20"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="49"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU003750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF11723.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="210" mass="22498" checksum="FF241E562F26817C" modified="2006-05-30" version="1">MSTPQPRPRHFSMLRDLQLADFFTLANAACGMGSVFFAMFFVAEPSLRTFYIAAALAPAAFIFDVLDGRIARWRHAHSALGRELDSLSDIISFGVGPATLAFAAGMRGGWDLAALIYFVCCGVSRLARFNVTAESMAEGTGKVKYFEGTPIPTSVVLTGVLAWCASQGRIGDALVGGAWEIGPGTLHPMALLFVLSGSLMISKTLRIPKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LDW7</accession>
    <name>Q1LDW7_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Rhs-related transmembrane protein, YD repeat protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">rhsC</name>
        <name evidence="3" type="ordered locus">Rmet_4797</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11659.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519225.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4797"/>
    <dbReference type="EnsemblBacteria" id="ABF11659">
        <property type="protein sequence ID" value="ABF11659"/>
        <property type="gene ID" value="Rmet_4797"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4797"/>
    <dbReference type="eggNOG" id="COG3209">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001218_1_0_4"/>
    <dbReference type="OMA" id="DGKGTHR"/>
    <dbReference type="OrthoDB" id="1639867at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045351">
        <property type="entry name" value="DUF6531"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001826">
        <property type="entry name" value="RHS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022385">
        <property type="entry name" value="Rhs_assc_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031325">
        <property type="entry name" value="RHS_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006530">
        <property type="entry name" value="YD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20148">
        <property type="entry name" value="DUF6531"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03527">
        <property type="entry name" value="RHS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05593">
        <property type="entry name" value="RHS_repeat"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03696">
        <property type="entry name" value="Rhs_assc_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01643">
        <property type="entry name" value="YD_repeat_2x"/>
        <property type="match status" value="7"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <feature type="domain" description="DUF6531" evidence="2">
        <location>
            <begin position="327"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="271"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1116"/>
            <end position="1138"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1603"/>
            <end position="1626"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="291"/>
            <end position="306"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF20148"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF11659.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="1626" mass="181650" checksum="8BA5F5F2F6512D35" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LDZ5</accession>
    <name>Q1LDZ5_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">2-Keto-3-deoxygluconate kinase</fullName>
            <ecNumber evidence="2">2.7.1.45</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">kdgK</name>
        <name evidence="2" type="ordered locus">Rmet_4769</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EC" id="2.7.1.45" evidence="2"/>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11631.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519201.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4769"/>
    <dbReference type="EnsemblBacteria" id="ABF11631">
        <property type="protein sequence ID" value="ABF11631"/>
        <property type="gene ID" value="Rmet_4769"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4769"/>
    <dbReference type="eggNOG" id="COG0524">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027634_6_0_4"/>
    <dbReference type="OMA" id="MAMFYAN"/>
    <dbReference type="OrthoDB" id="1604782at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008673">
        <property type="term" value="F:2-dehydro-3-deoxygluconokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1190.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011611">
        <property type="entry name" value="PfkB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029056">
        <property type="entry name" value="Ribokinase-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00294">
        <property type="entry name" value="PfkB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53613">
        <property type="entry name" value="SSF53613"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0418">Kinase</keyword>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PfkB" evidence="1">
        <location>
            <begin position="5"/>
            <end position="295"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF11631.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="311" mass="33353" checksum="53033D69E807050D" modified="2006-05-30" version="1">MSIDILAYGEPLIEFNQQPDDPSRYLQGFGGDTSNFCIAAARQGATTGYITAVGADSFGERLLALWTRERVDTRHVVVNASAPTGVYFVSHDNHGHRFDYLRERSAASRYHHEQLPLATIAQARFLHLSGISLAISTSACDAGLAAMEHARKAGAKVSLDTNLRLRLWSLARARGIMREAFSLTDICLPSWDDITILTGLDDRDAIVDYLLGCGVELVALKLGEEGSYIATQESRALVAPFPVRPVDATGAGDCFGGSFIARLAADADPFDAARYANIAAALSTTGYGAVAPIPHAETVRARLEQGVTVIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LDZ8</accession>
    <name>Q1LDZ8_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative diguanylate cyclase/phosphodiesterase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_4766</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11628.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519199.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4766"/>
    <dbReference type="EnsemblBacteria" id="ABF11628">
        <property type="protein sequence ID" value="ABF11628"/>
        <property type="gene ID" value="Rmet_4766"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4766"/>
    <dbReference type="eggNOG" id="COG2199">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG2200">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_70_50_4"/>
    <dbReference type="OMA" id="YTVRLRW"/>
    <dbReference type="OrthoDB" id="1635706at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="CDD" id="cd01948">
        <property type="entry name" value="EAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.450">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001633">
        <property type="entry name" value="EAL_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035919">
        <property type="entry name" value="EAL_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029787">
        <property type="entry name" value="Nucleotide_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00563">
        <property type="entry name" value="EAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00052">
        <property type="entry name" value="EAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF141868">
        <property type="entry name" value="SSF141868"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55073">
        <property type="entry name" value="SSF55073"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50883">
        <property type="entry name" value="EAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="EAL" evidence="2">
        <location>
            <begin position="181"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF11628.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="445" mass="48147" checksum="6158DF552198AAD8" modified="2006-05-30" version="1">MDSEAKDAHWGDDRAREPALSESQPAAFLAALAGHRAEIGTAHPLAIVIVRLDRFQFACETIGSRRTRLLRAEVKSRIAGVGVMPAVMHWLSPADLGIACLLPATAEEMAELSQSIATALRLPYSVDGFELFLSCSIGTAIDSPESATERNLQQAFDAMLQINRRGGGGIANASQPVSPRMVTLLAALPEAVSRGEFSLQLQPRALFDTAEIAAYTVRLRWHHPVLGRISPQDFLPAVEALGMMREVGHWLVQQLLQLIQESKHVAKVQFTLLASSPQLLADDAITMLQRAVETSGISPDRLCVEVPVQCISAVADATQKAARLHDCGIQISLSDFTDDQVSRHALALVAPDMVMLDARHLGSSAQARDAATLLRAACVFARSRGVAVHARGVETRAQLEAVRDWGCDGVQGYLLAQPFPAQWLVQTHAAVAERARQLLRPTNLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LE97</accession>
    <name>Q1LE97_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Hydrogenase-4 component G</fullName>
            <ecNumber evidence="3">1.6.99.5</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">hyfG</name>
        <name evidence="3" type="ordered locus">Rmet_4667</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EC" id="1.6.99.5" evidence="3"/>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11529.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519100.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4667"/>
    <dbReference type="EnsemblBacteria" id="ABF11529">
        <property type="protein sequence ID" value="ABF11529"/>
        <property type="gene ID" value="Rmet_4667"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4667"/>
    <dbReference type="eggNOG" id="COG3261">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG3262">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015134_3_1_4"/>
    <dbReference type="OMA" id="WAYCMAL"/>
    <dbReference type="OrthoDB" id="473681at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008137">
        <property type="term" value="F:NADH dehydrogenase (ubiquinone) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048038">
        <property type="term" value="F:quinone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.645.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001135">
        <property type="entry name" value="NADH_Q_OxRdtase_suD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037232">
        <property type="entry name" value="NADH_quin_OxRdtase_su_C/D-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001268">
        <property type="entry name" value="NADH_UbQ_OxRdtase_30kDa_su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029014">
        <property type="entry name" value="NiFe-Hase_large"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00329">
        <property type="entry name" value="Complex1_30kDa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00346">
        <property type="entry name" value="Complex1_49kDa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143243">
        <property type="entry name" value="SSF143243"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56762">
        <property type="entry name" value="SSF56762"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Complex1_30kDa" evidence="1">
        <location>
            <begin position="54"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="domain" description="Complex1_49kDa" evidence="2">
        <location>
            <begin position="281"/>
            <end position="435"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00346"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF11529.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="514" mass="55800" checksum="502E2AFF09B77DB0" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-10-07" version="52" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LES1</accession>
    <name>Q1LES1_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_4490</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11355.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4490"/>
    <dbReference type="EnsemblBacteria" id="ABF11355">
        <property type="protein sequence ID" value="ABF11355"/>
        <property type="gene ID" value="Rmet_4490"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4490"/>
    <dbReference type="HOGENOM" id="CLU_2481055_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="63"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF11355.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="87" mass="9275" checksum="B5B11BBF175EC6F3" modified="2006-05-30" version="1">MRGSIMQERTKAVLAIRVACVAAVCVVLANYLLDAAKACKLNRGLFMIDQQTGTSWCVPYQTLGGGLLLLSLFLIAVGVLLAFSKGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="58" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LF66</accession>
    <name>Q1LF66_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_4345</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11210.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518832.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF11210">
        <property type="protein sequence ID" value="ABF11210"/>
        <property type="gene ID" value="Rmet_4345"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4345"/>
    <dbReference type="HOGENOM" id="CLU_2013387_0_0_4"/>
    <dbReference type="OMA" id="QATAQNE"/>
    <dbReference type="OrthoDB" id="1748053at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF11210.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="123" mass="13442" checksum="FD58C43D4417F756" modified="2006-05-30" version="1">MAKKSIRVSHVAIPGDLSVLKLKGYLRSALTEVAEGTGDDEILLIKVLVPRPLGLKAGEKLLDKVLQAIVDKDPRVSRVSVEFVDGDVTPEKIAESQARTQKEIDAYGHLLQESTDEGESDRH</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LFF0</accession>
    <name>Q1LFF0_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Pirin-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Rmet_4260</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="6">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="6">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the pirin family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11126.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518752.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4260"/>
    <dbReference type="EnsemblBacteria" id="ABF11126">
        <property type="protein sequence ID" value="ABF11126"/>
        <property type="gene ID" value="Rmet_4260"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4260"/>
    <dbReference type="eggNOG" id="COG1741">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045717_5_1_4"/>
    <dbReference type="OMA" id="GWSACSY"/>
    <dbReference type="OrthoDB" id="833478at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012093">
        <property type="entry name" value="Pirin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008778">
        <property type="entry name" value="Pirin_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003829">
        <property type="entry name" value="Pirin_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011051">
        <property type="entry name" value="RmlC_Cupin_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02678">
        <property type="entry name" value="Pirin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05726">
        <property type="entry name" value="Pirin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006232">
        <property type="entry name" value="Pirin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51182">
        <property type="entry name" value="SSF51182"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0614">Plasmid</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pirin" evidence="3">
        <location>
            <begin position="20"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="domain" description="Pirin_C" evidence="4">
        <location>
            <begin position="182"/>
            <end position="284"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008416"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU003457"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF02678"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF05726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABF11126.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="293" mass="31446" checksum="99339FDEDE8B6438" modified="2006-05-30" version="1">MKRVLGVYSAPRSHWVGDGFPVRSMFSYQSHGKRLSPFLLLDYAGPADFSPGQQPRGVGVHPHRGFETVTIVYKGEVAHRDSTGQGGVIGAGDVQWMTAGGGILHEEFHSEAFTRTGGPLEMVQLWVNLPARHKMTAPGYQAIVDADIPVVPLRDDAGTVRVIAGQYEGKVGPAHTFTPMNVWDMRLNQGGISELPIPSGWNAALIVLRGTVRVNVSTVAGEAQMVLLDRVGEDVSMEASTDAVLILLAGEPIDEPIIGYGPFVMNSQDEILQAFTDLNGGRFGTVAQQTTSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LFG2</accession>
    <name>Q1LFG2_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_4248</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11114.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518739.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF11114">
        <property type="protein sequence ID" value="ABF11114"/>
        <property type="gene ID" value="Rmet_4248"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4248"/>
    <dbReference type="HOGENOM" id="CLU_1884065_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF11114.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="135" mass="15049" checksum="75A6AF1564F11959" modified="2006-05-30" version="1">MTVHPTRIAAQRILAVLQQGRILSRIDMMQMGLVATPREAEGALEILRAAGTIRRSRVSASCAAKDTRYEATGFTLREPRPKPSAASFDALLQVWHIALPKAPRRGHVRDMFAQEEKRYLAEELELGTNGENRQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="72" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LFQ1</accession>
    <name>Q1LFQ1_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">pelD</name>
        <name evidence="4" type="ordered locus">Rmet_4158</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="5">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="5">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11025.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518647.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4158"/>
    <dbReference type="EnsemblBacteria" id="ABF11025">
        <property type="protein sequence ID" value="ABF11025"/>
        <property type="gene ID" value="Rmet_4158"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4158"/>
    <dbReference type="eggNOG" id="COG2203">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046136_1_0_4"/>
    <dbReference type="OMA" id="NHYLLRI"/>
    <dbReference type="OrthoDB" id="1776821at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.2880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003018">
        <property type="entry name" value="GAF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029016">
        <property type="entry name" value="GAF-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031583">
        <property type="entry name" value="PelD_GGDEF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038367">
        <property type="entry name" value="PelD_GGDEF_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13492">
        <property type="entry name" value="GAF_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16963">
        <property type="entry name" value="PelD_GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0614">Plasmid</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="21"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="67"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="108"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="domain" description="GAF" evidence="2">
        <location>
            <begin position="205"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="domain" description="PelD_GGDEF" evidence="3">
        <location>
            <begin position="333"/>
            <end position="457"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF16963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF11025.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="487" mass="52630" checksum="025B229A039170E3" modified="2006-05-30" version="1">MAKTVETRRQGQGIGLGGRYARWLAPAGGGAAAVIEMIVIMAAAMGITWLVLPQNPLLLGMGFPWAWLLPVILALRYGTLVGVGAVLMLLGGWFFFDEIGAHSGAFPRMFFMGGLLLVLVAGQFGDVWNTRLARARAVNRYLDERLAALTKNHYLLRISHARLENDLLARPTTLRDTLSQLRAVALQDAMNGGKSGPLAGAQPMLQVVAQACQVEGAALYACDGERVVPNAVASIGPAFEIDVNDPLVRHCLETRALAHLRSTGLQQDAQTRYVAVAPVLAGSDRLIGVLVVERMPFLSLTYENLQMLMVLMGYYADGVEHARATHGIQEMAPAIPYAFALDYARLSRLRHETGIQSSVVALVFDLDEARDALFEQVVRSRRALDVAWPVRNAHHRAMLTLMPLSDAQAVSAYLVRIEDMLRAQFGTDFSSAHIGVYTLSVPAEGAEEALMRLLHRCQLDGASTITAVQADGTRDASVASAGPARAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-10-07" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LG93</accession>
    <name>Q1LG93_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_3965</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10833.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3965"/>
    <dbReference type="EnsemblBacteria" id="ABF10833">
        <property type="protein sequence ID" value="ABF10833"/>
        <property type="gene ID" value="Rmet_3965"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3965"/>
    <dbReference type="eggNOG" id="COG1695">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_155886_0_0_4"/>
    <dbReference type="OMA" id="MIRIPAP"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005149">
        <property type="entry name" value="Tscrpt_reg_PadR_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03551">
        <property type="entry name" value="PadR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PadR" evidence="1">
        <location>
            <begin position="29"/>
            <end position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF10833.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="113" mass="12683" checksum="A33D23928554B353" modified="2006-05-30" version="1">MIRFSAPLVALPAPMKTPSLDRIVSRLYILRLVQASPSTVFNLMERLRERGIDKNIRALRPILRSLMMARSITAELVEGNGRVYSITDAGRAELDAYLAHLNVLQDDMSETAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LGD1</accession>
    <name>Q1LGD1_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Extra-cytoplasmic solute receptor protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">bug</name>
        <name evidence="3" type="ordered locus">Rmet_3927</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Periplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the UPF0065 (bug) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10795.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518443.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3927"/>
    <dbReference type="EnsemblBacteria" id="ABF10795">
        <property type="protein sequence ID" value="ABF10795"/>
        <property type="gene ID" value="Rmet_3927"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3927"/>
    <dbReference type="eggNOG" id="COG3181">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045683_0_1_4"/>
    <dbReference type="OMA" id="YPGFEMT"/>
    <dbReference type="OrthoDB" id="447734at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042597">
        <property type="term" value="C:periplasmic space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005064">
        <property type="entry name" value="BUG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042100">
        <property type="entry name" value="Bug_dom1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006311">
        <property type="entry name" value="TAT_signal"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42928">
        <property type="entry name" value="PTHR42928"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03401">
        <property type="entry name" value="TctC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017082">
        <property type="entry name" value="YflP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51318">
        <property type="entry name" value="TAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-0675">Receptor</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF10795.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="329" mass="34488" checksum="F4A0E308D59B9B43" modified="2006-05-30" version="1">MSQFDSSRRRVLGATGALAAAAMLPSLSRAQTWPSKTIRFVVPFAPGGSSEIIARSVAAELTSQLGVSVFVENKPGGAGNVAMGEVARADDQHTIILGHIGTLAVNPFIFPKLPYDVNKDFTPVSLLAKVPSLYVVHPDVPAKNLKEFVALAKSKPGKLNYGSAGNGSAGHLAFEYLKEAAGIFVLHVPYRGSGPQITDLLSGRLEAAAVGAPAILPFIKSGKLRCIATGSAQRIPQLPDVPTVAEQGYPGFEMTQWYGILAPSSMSKAAVDKLAAASAKSVRSPAASSRMTADAAIVVGDTPAEFASFIAQEQKRWKPIIARASIKPD</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="95" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LGD6</accession>
    <name>Q1LGD6_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DNA-directed DNA polymerase</fullName>
            <ecNumber evidence="1">2.7.7.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">ydjQ</name>
        <name evidence="5" type="ordered locus">Rmet_3922</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="6">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="6">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) = diphosphate + DNA(n+1)</text>
            <dbReference type="Rhea" id="RHEA:22508"/>
            <dbReference type="Rhea" id="RHEA-COMP:17339"/>
            <dbReference type="Rhea" id="RHEA-COMP:17340"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61560"/>
            <dbReference type="ChEBI" id="CHEBI:173112"/>
            <dbReference type="EC" id="2.7.7.7"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.7.7.7" evidence="1"/>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10790.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518437.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3922"/>
    <dbReference type="EnsemblBacteria" id="ABF10790">
        <property type="protein sequence ID" value="ABF10790"/>
        <property type="gene ID" value="Rmet_3922"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3922"/>
    <dbReference type="eggNOG" id="COG0322">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG0847">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030720_0_0_4"/>
    <dbReference type="OMA" id="AVDHVEC"/>
    <dbReference type="OrthoDB" id="1985371at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003887">
        <property type="term" value="F:DNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004527">
        <property type="term" value="F:exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1440.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006054">
        <property type="entry name" value="DnaQ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013520">
        <property type="entry name" value="Exonuclease_RNaseT/DNA_pol3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000305">
        <property type="entry name" value="GIY-YIG_endonuc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035901">
        <property type="entry name" value="GIY-YIG_endonuc_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01541">
        <property type="entry name" value="GIY-YIG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00929">
        <property type="entry name" value="RNase_T"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00479">
        <property type="entry name" value="EXOIII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00465">
        <property type="entry name" value="GIYc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="SSF53098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82771">
        <property type="entry name" value="SSF82771"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00573">
        <property type="entry name" value="dnaq"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50164">
        <property type="entry name" value="GIY_YIG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="5" id="KW-0614">Plasmid</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="GIY-YIG" evidence="4">
        <location>
            <begin position="228"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="504"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="516"/>
            <end position="530"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00024632"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50164"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABF10790.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="530" mass="59081" checksum="26BFDB40DC15579A" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="98" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LGI8</accession>
    <name>Q1LGI8_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_3870</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the LysR transcriptional regulatory family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10738.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518385.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3870"/>
    <dbReference type="EnsemblBacteria" id="ABF10738">
        <property type="protein sequence ID" value="ABF10738"/>
        <property type="gene ID" value="Rmet_3870"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3870"/>
    <dbReference type="eggNOG" id="COG0583">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039613_16_2_4"/>
    <dbReference type="OMA" id="PEWEFIS"/>
    <dbReference type="OrthoDB" id="1661500at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005119">
        <property type="entry name" value="LysR_subst-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000847">
        <property type="entry name" value="Tscrpt_reg_HTH_LysR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00126">
        <property type="entry name" value="HTH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03466">
        <property type="entry name" value="LysR_substrate"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50931">
        <property type="entry name" value="HTH_LYSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH lysR-type" evidence="2">
        <location>
            <begin position="1"/>
            <end position="59"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF10738.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="289" mass="32231" checksum="5551250E38B3EC1B" modified="2006-05-30" version="1">MELLNDMALFVEVVKAKGFRSAADVLDMPSSTLSRRISALEKAIGLRLLHRTTRKIELTEAGRIYFERCKRIVGDARLAHEQLGTMLAEPVGVLRASFPVDFAVTYLAPLIAEFAGRYPGLSFDFDLTPRRVDLVSEPFDVAIRAGEPEVSQLIARQLASFRTYLYASPGYIKRAGEPRAPADLNHHECLGIQRGGAWTLHRGEDTTTIPVGSRFTLNSVGMARRLAALDMGIVLMAEAIVADDLATGQLRRLMPEWQGTPVPVYAMTETRLLPAKTQRFIEFLRERFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LGJ1</accession>
    <name>Q1LGJ1_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_3867</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10735.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518382.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3867"/>
    <dbReference type="EnsemblBacteria" id="ABF10735">
        <property type="protein sequence ID" value="ABF10735"/>
        <property type="gene ID" value="Rmet_3867"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3867"/>
    <dbReference type="eggNOG" id="COG2207">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_879183_0_0_4"/>
    <dbReference type="OMA" id="HLAPRWR"/>
    <dbReference type="OrthoDB" id="1757088at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018060">
        <property type="entry name" value="HTH_AraC"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01124">
        <property type="entry name" value="HTH_ARAC_FAMILY_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH araC/xylS-type" evidence="2">
        <location>
            <begin position="221"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS01124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF10735.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="312" mass="34724" checksum="1EE7C98CE69AE22B" modified="2006-05-30" version="1">MSSGPLSTPIQCPDPAARAPLASHGGRTPHARLYPAPPSLHGAIAAIVCRDTRGFPLTDAQRLTHVPASPLVCLSWFPDPGTGLVERSSNGPLWRPFGATVTVSGSQSSPLTSWAPTTGRGGMVCFTADVAKALFDLDLTAIHDRFRCAREVLGDGWRPLIESLSDAESDAETLAALEQHLAPRWHALRHRSSPLASLRQTGRRWVESLAWQAREWQRTSSPRQVERRIKAYSGRSLREWQAIVRTEGAFFTARDRYEAGQPFDWPGLALEEGFADQAHLVRTTKRITGFSPTEFTQRFVEDESFWLYRLWV</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-10-07" version="58" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LGU6</accession>
    <name>Q1LGU6_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">bPH_2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_3759</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10630.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3759"/>
    <dbReference type="TCDB" id="9.B.154.1.4">
        <property type="family name" value="the putative holin-2 (ph-2) family"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF10630">
        <property type="protein sequence ID" value="ABF10630"/>
        <property type="gene ID" value="Rmet_3759"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3759"/>
    <dbReference type="eggNOG" id="COG3428">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1947046_0_0_4"/>
    <dbReference type="OMA" id="THTERMR"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005182">
        <property type="entry name" value="DUF304"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03703">
        <property type="entry name" value="bPH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="bPH_2" evidence="1">
        <location>
            <begin position="28"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF10630.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="129" mass="14480" checksum="D36D5D1E6487F6D2" modified="2006-05-30" version="1">MLCVVMCFIPFLIPAAVIIAALSYLRIQTTRIEVTTKRVTVRSGIMSMKLDEVELSLVRDIQLTQPLLLWLLKLANIAIVSSNGASPDIHLHGIPNAYVLREKIRRCVENHRDSKGVRDSESRHLVAHA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-10-03" modified="2022-05-25" version="72" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LH12</accession>
    <name>CHED_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Probable chemoreceptor glutamine deamidase CheD</fullName>
            <ecNumber evidence="1">3.5.1.44</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">cheD</name>
        <name type="ordered locus">Rmet_3692</name>
    </gene>
    <organism>
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid">
        <name>megaplasmid</name>
    </geneLocation>
    <reference key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Probably deamidates glutamine residues to glutamate on methyl-accepting chemotaxis receptors (MCPs), playing an important role in chemotaxis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>H2O + L-glutaminyl-[protein] = L-glutamyl-[protein] + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:16441"/>
            <dbReference type="Rhea" id="RHEA-COMP:10207"/>
            <dbReference type="Rhea" id="RHEA-COMP:10208"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:29973"/>
            <dbReference type="ChEBI" id="CHEBI:30011"/>
            <dbReference type="EC" id="3.5.1.44"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the CheD family.</text>
    </comment>
    <dbReference type="EC" id="3.5.1.44" evidence="1"/>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10564.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q1LH12"/>
    <dbReference type="SMR" id="Q1LH12"/>
    <dbReference type="STRING" id="266264.Rmet_3692"/>
    <dbReference type="EnsemblBacteria" id="ABF10564">
        <property type="protein sequence ID" value="ABF10564"/>
        <property type="gene ID" value="Rmet_3692"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3692"/>
    <dbReference type="eggNOG" id="COG1871">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_087854_0_0_4"/>
    <dbReference type="OMA" id="VGMADLN"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050568">
        <property type="term" value="F:protein-glutamine glutaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006935">
        <property type="term" value="P:chemotaxis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd16352">
        <property type="entry name" value="CheD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01440">
        <property type="entry name" value="CheD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038592">
        <property type="entry name" value="CheD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005659">
        <property type="entry name" value="Chemorcpt_Glu_NH3ase_CheD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011324">
        <property type="entry name" value="Cytotoxic_necrot_fac-like_cat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35147">
        <property type="entry name" value="PTHR35147"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03975">
        <property type="entry name" value="CheD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64438">
        <property type="entry name" value="SSF64438"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0145">Chemotaxis</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0614">Plasmid</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000251054" description="Probable chemoreceptor glutamine deamidase CheD">
        <location>
            <begin position="1"/>
            <end position="220"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01440"/>
        </source>
    </evidence>
    <sequence length="220" mass="24534" checksum="2856CD64569503FC" modified="2006-05-30" version="1">MMRGTPQMPEAIATRKYFDREFDRQAIKLLPNEYYVTAEDVVLTTVLGSCVSACIRDEVAGVGGMNHFMLPDDENAGADRMLSTSMRYGCYALEVLINELLKMGARRERLEAKVFGGGAVLANMTTLNIGDRNADFVLKYLRTEEVRVAAQDLRGPHARRVSYFPRTGLALVRRLTRQDDTVGIEREERALARALSTSTKAPARGAVELFTRQTTARIPT</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LH25</accession>
    <name>Q1LH25_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Diguanylate cyclase/phosphodiesterase (GGDEF and EAL domains)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Rmet_3679</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="6">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="6">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10551.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518202.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3679"/>
    <dbReference type="EnsemblBacteria" id="ABF10551">
        <property type="protein sequence ID" value="ABF10551"/>
        <property type="gene ID" value="Rmet_3679"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3679"/>
    <dbReference type="eggNOG" id="COG2199">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG2200">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_70_49_4"/>
    <dbReference type="OMA" id="MTARACI"/>
    <dbReference type="OrthoDB" id="1635706at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01948">
        <property type="entry name" value="EAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01949">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.450">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001633">
        <property type="entry name" value="EAL_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035919">
        <property type="entry name" value="EAL_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000160">
        <property type="entry name" value="GGDEF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029787">
        <property type="entry name" value="Nucleotide_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00563">
        <property type="entry name" value="EAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00990">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00052">
        <property type="entry name" value="EAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00267">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF141868">
        <property type="entry name" value="SSF141868"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55073">
        <property type="entry name" value="SSF55073"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00254">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50883">
        <property type="entry name" value="EAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50887">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0614">Plasmid</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193120" evidence="2">
        <location>
            <begin position="26"/>
            <end position="660"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="41"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="72"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="105"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="148"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="domain" description="GGDEF" evidence="4">
        <location>
            <begin position="260"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="domain" description="EAL" evidence="3">
        <location>
            <begin position="402"/>
            <end position="656"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABF10551.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="660" mass="70069" checksum="8E73F0C0F4D4A50A" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LH27</accession>
    <name>Q1LH27_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Histone-like nucleoid-structuring protein HNS-like: transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">stpA</name>
        <name evidence="6" type="ordered locus">Rmet_3677</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="7">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="7">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Nucleoid</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the histone-like protein H-NS family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10549.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518200.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3677"/>
    <dbReference type="EnsemblBacteria" id="ABF10549">
        <property type="protein sequence ID" value="ABF10549"/>
        <property type="gene ID" value="Rmet_3677"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3677"/>
    <dbReference type="eggNOG" id="COG2916">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_117503_4_1_4"/>
    <dbReference type="OMA" id="QGNTWTG"/>
    <dbReference type="OrthoDB" id="1911889at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009295">
        <property type="term" value="C:nucleoid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030527">
        <property type="term" value="F:structural constituent of chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.430.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027444">
        <property type="entry name" value="H-NS_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037150">
        <property type="entry name" value="H-NS_C_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001801">
        <property type="entry name" value="Histone_HNS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00816">
        <property type="entry name" value="Histone_HNS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00528">
        <property type="entry name" value="HNS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0614">Plasmid</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HNS" evidence="5">
        <location>
            <begin position="58"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004453"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010610"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABF10549.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="102" mass="11340" checksum="D8C6D9F261E91219" modified="2006-05-30" version="1">MATYQEIVQKISELQKQAEEIKAREQASVIADIREKIEQYGLTAEDLGFGGKAVAGKKAVSRKVPVRYRDSAGNTWTGRGKRPGWLTQALANGKSMEDFLIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LHV0</accession>
    <name>Q1LHV0_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">3-methyl-adenine DNA glycosylase I, constitutive</fullName>
            <ecNumber evidence="1">3.2.2.20</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">tag</name>
        <name evidence="1" type="ordered locus">Rmet_3404</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EC" id="3.2.2.20" evidence="1"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10276.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517840.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3404"/>
    <dbReference type="EnsemblBacteria" id="ABF10276">
        <property type="protein sequence ID" value="ABF10276"/>
        <property type="gene ID" value="Rmet_3404"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3404"/>
    <dbReference type="eggNOG" id="COG2818">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_083758_0_0_4"/>
    <dbReference type="OMA" id="YVAYHDT"/>
    <dbReference type="OrthoDB" id="1835478at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008725">
        <property type="term" value="F:DNA-3-methyladenine glycosylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006284">
        <property type="term" value="P:base-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005019">
        <property type="entry name" value="Adenine_glyco"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011257">
        <property type="entry name" value="DNA_glycosylase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03352">
        <property type="entry name" value="Adenine_glyco"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48150">
        <property type="entry name" value="SSF48150"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF10276.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="208" mass="23914" checksum="28798723800235CF" modified="2006-05-30" version="1">MTRSTSAPQPRERCGWCGTLEDYCHYHDHEWGFPVDDDRRLFEKLCLEGFQSGLSWLTILRKREAFRSAFANFEINKVARFTEHDVERLLGDASIVRHRGKIEAAINNAQCAKELLKTESSLAAYFWKFEPDPKSRPKTLTAEVLQGMTTCPEAVALSKDLKKRGWKFVGPTTMYALMQAMGLVNDHREGCWTRAEVQRARKAFKVPK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2007-07-10" modified="2022-05-25" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LI62</accession>
    <name>RS4_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">30S ribosomal protein S4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpsD</name>
        <name type="ordered locus">Rmet_3292</name>
    </gene>
    <organism>
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">One of the primary rRNA binding proteins, it binds directly to 16S rRNA where it nucleates assembly of the body of the 30S subunit.</text>
    </comment>
    <comment type="function">
        <text evidence="1">With S5 and S12 plays an important role in translational accuracy.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the 30S ribosomal subunit. Contacts protein S5. The interaction surface between S4 and S5 is involved in control of translational fidelity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uS4 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10164.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517763.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q1LI62"/>
    <dbReference type="SMR" id="Q1LI62"/>
    <dbReference type="STRING" id="266264.Rmet_3292"/>
    <dbReference type="EnsemblBacteria" id="ABF10164">
        <property type="protein sequence ID" value="ABF10164"/>
        <property type="gene ID" value="Rmet_3292"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3292"/>
    <dbReference type="eggNOG" id="COG0522">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_092403_0_2_4"/>
    <dbReference type="OMA" id="NVVFRMG"/>
    <dbReference type="OrthoDB" id="1211060at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015935">
        <property type="term" value="C:small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00165">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.290.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01306_B">
        <property type="entry name" value="Ribosomal_S4_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022801">
        <property type="entry name" value="Ribosomal_S4/S9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001912">
        <property type="entry name" value="Ribosomal_S4/S9_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005709">
        <property type="entry name" value="Ribosomal_S4_bac-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018079">
        <property type="entry name" value="Ribosomal_S4_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002942">
        <property type="entry name" value="S4_RNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036986">
        <property type="entry name" value="S4_RNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11831">
        <property type="entry name" value="PTHR11831"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11831:SF4">
        <property type="entry name" value="PTHR11831:SF4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00163">
        <property type="entry name" value="Ribosomal_S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01479">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01390">
        <property type="entry name" value="Ribosomal_S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00363">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01017">
        <property type="entry name" value="rpsD_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00632">
        <property type="entry name" value="RIBOSOMAL_S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50889">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <feature type="chain" id="PRO_0000293347" description="30S ribosomal protein S4">
        <location>
            <begin position="1"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="domain" description="S4 RNA-binding" evidence="1">
        <location>
            <begin position="97"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="31"/>
            <end position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="207" mass="23277" checksum="42970FA304357141" modified="2006-05-30" version="1">MARYIGPKAKLSRREGTDLFLKSARRSLADKCKLDSKPGQHGRTSGARTSDYGNQLREKQKVKRIYGVLERQFRRYFAEADRRKGNTGENLLQLLESRLDNVVYRMGFGSTRAEARQLVSHKAILVNGETLNVPSAQIKSGDIVSVREQSKKQVRIAEALSLAEQSGFPTWVAVDAKKFEGTYKQAPDRADISGDINESLIVELYSR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2007-06-12" modified="2022-05-25" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LIB1</accession>
    <name>HIS4_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase</fullName>
            <ecNumber evidence="1">5.3.1.16</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Phosphoribosylformimino-5-aminoimidazole carboxamide ribotide isomerase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">hisA</name>
        <name type="ordered locus">Rmet_3243</name>
    </gene>
    <organism>
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>1-(5-phospho-beta-D-ribosyl)-5-[(5-phospho-beta-D-ribosylamino)methylideneamino]imidazole-4-carboxamide = 5-[(5-phospho-1-deoxy-D-ribulos-1-ylimino)methylamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide</text>
            <dbReference type="Rhea" id="RHEA:15469"/>
            <dbReference type="ChEBI" id="CHEBI:58435"/>
            <dbReference type="ChEBI" id="CHEBI:58525"/>
            <dbReference type="EC" id="5.3.1.16"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-histidine biosynthesis; L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 4/9.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the HisA/HisF family.</text>
    </comment>
    <dbReference type="EC" id="5.3.1.16" evidence="1"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10115.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008643054.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q1LIB1"/>
    <dbReference type="SMR" id="Q1LIB1"/>
    <dbReference type="STRING" id="266264.Rmet_3243"/>
    <dbReference type="EnsemblBacteria" id="ABF10115">
        <property type="protein sequence ID" value="ABF10115"/>
        <property type="gene ID" value="Rmet_3243"/>
    </dbReference>
    <dbReference type="GeneID" id="60825567"/>
    <dbReference type="KEGG" id="rme:Rmet_3243"/>
    <dbReference type="eggNOG" id="COG0106">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_048577_1_1_4"/>
    <dbReference type="OMA" id="EWLHLVD"/>
    <dbReference type="OrthoDB" id="794219at2"/>
    <dbReference type="UniPathway" id="UPA00031">
        <property type="reaction ID" value="UER00009"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003949">
        <property type="term" value="F:1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino]imidazole-4-carboxamide isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000105">
        <property type="term" value="P:histidine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd04732">
        <property type="entry name" value="HisA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01014">
        <property type="entry name" value="HisA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006062">
        <property type="entry name" value="His_biosynth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006063">
        <property type="entry name" value="HisA_bact_arch"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044524">
        <property type="entry name" value="Isoase_HisA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023016">
        <property type="entry name" value="Isoase_HisA-like_bact"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011060">
        <property type="entry name" value="RibuloseP-bd_barrel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43090">
        <property type="entry name" value="PTHR43090"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00977">
        <property type="entry name" value="His_biosynth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51366">
        <property type="entry name" value="SSF51366"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00007">
        <property type="entry name" value="TIGR00007"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0368">Histidine biosynthesis</keyword>
    <keyword id="KW-0413">Isomerase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000290518" description="1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase">
        <location>
            <begin position="1"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="1">
        <location>
            <position position="8"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="1">
        <location>
            <position position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01014"/>
        </source>
    </evidence>
    <sequence length="247" mass="26115" checksum="5136C5D81A713DDF" modified="2006-05-30" version="1">MLLIPAIDLKDGQCVRLKQGDMDQATVFSEDPAAMARHWVNQGARRLHLVDLNGAFVGKPRNEAAIKAIIAEVGDEIPVQLGGGIRDLNTIERWLDDGLSYVIIGTAAVKNPGFLKDACAAFGGHIIVGLDAKDGKVATDGWSKLTGHEVADLARKYEDYGVESIIYTDIGRDGMLQGINIDATVKLARSMSIPVIASGGLSNMADIDQLCAVEGEGVEGVICGRAIYSGDLNFAAAQAHADKLGAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LIG4</accession>
    <name>Q1LIG4_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Thiol:disulfide interchange protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">dsbC</name>
        <name evidence="9" type="ordered locus">Rmet_3190</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Required for disulfide bond formation in some periplasmic proteins. Acts by transferring its disulfide bond to other proteins and is reduced in the process.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Periplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the thioredoxin family. DsbC subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10062.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517671.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3190"/>
    <dbReference type="EnsemblBacteria" id="ABF10062">
        <property type="protein sequence ID" value="ABF10062"/>
        <property type="gene ID" value="Rmet_3190"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3190"/>
    <dbReference type="eggNOG" id="COG1651">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_083593_1_1_4"/>
    <dbReference type="OMA" id="QMIVYKA"/>
    <dbReference type="OrthoDB" id="1678187at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042597">
        <property type="term" value="C:periplasmic space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015036">
        <property type="term" value="F:disulfide oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016853">
        <property type="term" value="F:isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03020">
        <property type="entry name" value="DsbA_DsbC_DsbG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.450.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033954">
        <property type="entry name" value="DiS-bond_Isoase_DsbC/G"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018950">
        <property type="entry name" value="DiS-bond_isomerase_DsbC/G_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009094">
        <property type="entry name" value="DiS-bond_isomerase_DsbC/G_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012336">
        <property type="entry name" value="Thioredoxin-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017937">
        <property type="entry name" value="Thioredoxin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10411">
        <property type="entry name" value="DsbC_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13098">
        <property type="entry name" value="Thioredoxin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="SSF52833"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54423">
        <property type="entry name" value="SSF54423"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00194">
        <property type="entry name" value="THIOREDOXIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0413">Isomerase</keyword>
    <keyword evidence="4 7" id="KW-0574">Periplasm</keyword>
    <keyword evidence="6 7" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5010000265" description="Thiol:disulfide interchange protein" evidence="7">
        <location>
            <begin position="34"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="domain" description="Thioredoxin" evidence="8">
        <location>
            <begin position="56"/>
            <end position="252"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009813"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022764"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU364038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ABF10062.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="252" mass="27529" checksum="8B01E9CAA477F224" modified="2006-05-30" version="1">MFQMFRRRSARFAAIAALASGVGAAGYALHALAADEPGTDRIKASIQKILGGRAEVKSVTKAPVPGLYEVNVSGQLVYTDATGRYIINGELIDTKTNTNLTEERLAELNKIKWSDLPLSRAIKWTKGDGSRTVAVFSDPNCGYCKKIEQTFQQMDNITVYTFLYPVLSPDSTVKAKQVWCSADRTKVWRDWMLNHVALTGNGSCKTPVDENLELGHNLNITGTPAVFFSDGTRIPGAADAATLERKLASLKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LIG9</accession>
    <name>Q1LIG9_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">ABC-type transporter, permease component</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Rmet_3185</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cell membrane</location>
            <topology evidence="4">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the binding-protein-dependent transport system permease family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10057.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008647147.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3185"/>
    <dbReference type="EnsemblBacteria" id="ABF10057">
        <property type="protein sequence ID" value="ABF10057"/>
        <property type="gene ID" value="Rmet_3185"/>
    </dbReference>
    <dbReference type="GeneID" id="60825478"/>
    <dbReference type="KEGG" id="rme:Rmet_3185"/>
    <dbReference type="eggNOG" id="COG4986">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036171_2_0_4"/>
    <dbReference type="OMA" id="FGTQWYI"/>
    <dbReference type="OrthoDB" id="1065003at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="SSF161098"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2 4" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="27"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="75"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="115"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="192"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="245"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="344"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="373"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="418"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="444"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="491"/>
            <end position="512"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="549"/>
            <end position="568"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="5">
        <location>
            <begin position="70"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="5">
        <location>
            <begin position="381"/>
            <end position="567"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABF10057.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="581" mass="64157" checksum="C9959C68D63C327D" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LII9</accession>
    <name>Q1LII9_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Phenylacetate-coenzyme A ligase</fullName>
            <ecNumber evidence="1">6.2.1.30</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Phenylacetyl-CoA ligase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="4" type="primary">paaK</name>
        <name evidence="4" type="ordered locus">Rmet_3165</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the activation of phenylacetic acid (PA) to phenylacetyl-CoA (PA-CoA).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>2-phenylacetate + ATP + CoA = AMP + diphosphate + phenylacetyl-CoA</text>
            <dbReference type="Rhea" id="RHEA:20956"/>
            <dbReference type="ChEBI" id="CHEBI:18401"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57390"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.2.1.30"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Aromatic compound metabolism; phenylacetate degradation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the phenylacetyl-CoA ligase family.</text>
    </comment>
    <dbReference type="EC" id="6.2.1.30" evidence="1"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10037.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008647562.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3165"/>
    <dbReference type="EnsemblBacteria" id="ABF10037">
        <property type="protein sequence ID" value="ABF10037"/>
        <property type="gene ID" value="Rmet_3165"/>
    </dbReference>
    <dbReference type="GeneID" id="60825458"/>
    <dbReference type="KEGG" id="rme:Rmet_3165"/>
    <dbReference type="eggNOG" id="COG1541">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035301_1_1_4"/>
    <dbReference type="OMA" id="GVAPHFQ"/>
    <dbReference type="OrthoDB" id="839891at2"/>
    <dbReference type="UniPathway" id="UPA00930"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047475">
        <property type="term" value="F:phenylacetate-CoA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010124">
        <property type="term" value="P:phenylacetate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd05913">
        <property type="entry name" value="PaaK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045851">
        <property type="entry name" value="AMP-bd_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028154">
        <property type="entry name" value="AMP-dep_Lig_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_Synth/Lig"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011880">
        <property type="entry name" value="PA_CoA_ligase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14535">
        <property type="entry name" value="AMP-binding_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006444">
        <property type="entry name" value="PaaK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02155">
        <property type="entry name" value="PA_CoA_ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 4" id="KW-0436">Ligase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AMP-binding" evidence="2">
        <location>
            <begin position="85"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="domain" description="AMP-binding_C_2" evidence="3">
        <location>
            <begin position="339"/>
            <end position="432"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRNR" id="PIRNR006444"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF14535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF10037.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="434" mass="48001" checksum="21D446A1D0278039" modified="2006-05-30" version="1">MITRLTDLPLEPIETASRQELQALQLDRLRWSLRHAYDHSPVYRRKFDEAGVHPDQVRSLADLARFPFTTKKDLRDNYPFGMFAVPQDKIARIHASSGTTGKPTVVGYTLGDIDTWATVMARSIRASGARRGDKVHISYGYGLFTGGLGAHYGVEKAGLTAIPFGGGQTERQVQLIQDFKPEVIMVTPSYMLAIADELERQGVDPASTSLKIGIFGAEPWTPEMRLAIEKRMGISAVDIYGLSEVMGPGVANECAETKDGPTIWEDHFYPEIIDPDTGEVLPDGEFGELVFTSLTKEAMPVIRYRTRDLTRLMPGSARAAFRRMEKITGRTDDMMIVRGVNVFPSQIEELILKHAELAPHYQCVLNKDGPLDTLIVRVECAHGADPATARQAESQLAHDIKSYIGVTAGIEILAEGGVERSVGKARRIVDLRPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="60" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LIK7</accession>
    <name>Q1LIK7_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_3147</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10019.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517629.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3147"/>
    <dbReference type="EnsemblBacteria" id="ABF10019">
        <property type="protein sequence ID" value="ABF10019"/>
        <property type="gene ID" value="Rmet_3147"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3147"/>
    <dbReference type="eggNOG" id="ENOG50344XI">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1737501_0_0_4"/>
    <dbReference type="OMA" id="LAPSNEC"/>
    <dbReference type="OrthoDB" id="1764806at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193531" evidence="1">
        <location>
            <begin position="23"/>
            <end position="153"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF10019.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="153" mass="15518" checksum="CA56393BB8730DFB" modified="2006-05-30" version="1">MTQLLSRPLQLIGILSTACVMAACASSAPSGPQITPVNATVKLGRVTEKTFLTRIDVANPNDSGYGVGVGAAGVGGGGGGGGVGVGFSMDLTRLFNRPPPQQMDLYQYKIRAADGTVAQVNAPAAPGLEPGACVRLIYPDGSTQPQMAPSNEC</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="70" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LIN5</accession>
    <name>Q1LIN5_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Peptidase_M23 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_3119</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09991.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517614.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3119"/>
    <dbReference type="EnsemblBacteria" id="ABF09991">
        <property type="protein sequence ID" value="ABF09991"/>
        <property type="gene ID" value="Rmet_3119"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3119"/>
    <dbReference type="eggNOG" id="COG0739">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029425_2_2_4"/>
    <dbReference type="OMA" id="WQPGKRW"/>
    <dbReference type="OrthoDB" id="1891666at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.70.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011055">
        <property type="entry name" value="Dup_hybrid_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016047">
        <property type="entry name" value="Peptidase_M23"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01551">
        <property type="entry name" value="Peptidase_M23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51261">
        <property type="entry name" value="SSF51261"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="21"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase_M23" evidence="2">
        <location>
            <begin position="191"/>
            <end position="285"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF09991.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="310" mass="33691" checksum="927C61ACE8810083" modified="2006-05-30" version="1">MQIIVLHPREQRMLRFHLTRLRVALLMLALCSLVAAAASGVIWLLARSQASPEVSRQARENAFLRQNLAVLAASVGDLQAQMVRLDALGERVSGLAGIAPQDFDFRHRPARGGPAAPAQSPELTLPELRAELARLGEQAEHRVDYFDVIETALMDRQMRERRIPRVLPVATGYDGSSFGTRIDPFTGRRSQHEGVDFVAPTGTPILAAAGGVVVAAEWHNEYGNMIDIDHDNGLKTRYAHASKSLVRAGDIVRPGQMIARVGSTGRSTGAHLHFEVHVNGLPRNPNGFLNAAVPAPLAEALSPTNRTAIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LIY3</accession>
    <name>Q1LIY3_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Plasmid conjugal transfer transmembrane protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">traF</name>
        <name evidence="2" type="ordered locus">Rmet_3020</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09893.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517538.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3020"/>
    <dbReference type="MEROPS" id="S26.014"/>
    <dbReference type="EnsemblBacteria" id="ABF09893">
        <property type="protein sequence ID" value="ABF09893"/>
        <property type="gene ID" value="Rmet_3020"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3020"/>
    <dbReference type="eggNOG" id="COG4959">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_104604_0_0_4"/>
    <dbReference type="OMA" id="WYRIEPF"/>
    <dbReference type="OrthoDB" id="1741894at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006465">
        <property type="term" value="P:signal peptide processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036286">
        <property type="entry name" value="LexA/Signal_pep-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019533">
        <property type="entry name" value="Peptidase_S26"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10502">
        <property type="entry name" value="Peptidase_S26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51306">
        <property type="entry name" value="SSF51306"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <feature type="domain" description="Peptidase_S26" evidence="1">
        <location>
            <begin position="21"/>
            <end position="190"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF10502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF09893.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="195" mass="20887" checksum="07AA21225197DFD1" modified="2006-05-30" version="1">MTAVSTSGTAPHPRLRVRARLVLAGLSACGLAALAWASFVHPLPRLTYNPSDSVEVGWYRIDPLDHRTSSPPRPLSVGSIVLVPLPAEAAALAAQRGYLPTRIPLLKRVGAVAPQEVCIADGKVRIDGVPSAAVLSADRWGRPLPSRQQCHRLRHGELFLLSVTNPASFDSRYFGPVSAASVIGVARPVWLESRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="67" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LJ41</accession>
    <name>Q1LJ41_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_2962</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09835.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517498.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2962"/>
    <dbReference type="EnsemblBacteria" id="ABF09835">
        <property type="protein sequence ID" value="ABF09835"/>
        <property type="gene ID" value="Rmet_2962"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2962"/>
    <dbReference type="eggNOG" id="ENOG50318BZ">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_074587_2_0_4"/>
    <dbReference type="OMA" id="KFAYAAS"/>
    <dbReference type="OrthoDB" id="1970612at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010239">
        <property type="entry name" value="CHP02001"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09694">
        <property type="entry name" value="Gcw_chp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02001">
        <property type="entry name" value="gcw_chp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193157" evidence="1">
        <location>
            <begin position="30"/>
            <end position="265"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF09835.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="265" mass="28868" checksum="97825AEB368F5104" modified="2006-05-30" version="1">MIATRLLTSAPAGLAAAVLFAMPFSPAFAQEASAPVAEPSTPHTFTANVSLVSDYRYRGISQTNLRPAIQGGFDYSHASGFYVGNWNSSISWLEDADSSVSSPVEMDFYGGFRNTFKLSDLEFNYDVGVLEYYYPGGYTTTRPYTTELYAGIGYGPVFLKYSHAVTNLFGIDDSKNSYYVDLSANVPLNVWGLTFNAHVGYQGVAHHSDASYTDWKLGLTKDLGKGFSLAVAYVDTNAKASFYTSANRGRYLGKAAAWASITKTF</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LJ51</accession>
    <name>Q1LJ51_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Enzyme required for production of the extracellular factor (EF) 3-hydroxypalmitic acid methyl ester (3-OH PAME)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">phcB</name>
        <name evidence="2" type="ordered locus">Rmet_2952</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09825.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2952"/>
    <dbReference type="EnsemblBacteria" id="ABF09825">
        <property type="protein sequence ID" value="ABF09825"/>
        <property type="gene ID" value="Rmet_2952"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2952"/>
    <dbReference type="eggNOG" id="COG0500">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045659_0_0_4"/>
    <dbReference type="OMA" id="NPYSIVQ"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041698">
        <property type="entry name" value="Methyltransf_25"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13649">
        <property type="entry name" value="Methyltransf_25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Methyltransf_25" evidence="1">
        <location>
            <begin position="314"/>
            <end position="427"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF09825.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="489" mass="55836" checksum="3FD96F6E1D68D21A" modified="2006-05-30" version="1">MPELYPAGAHWRHGAQRFSDFPWRDTVYSKTQIDPVVSFRNSQGEQVRGTIINLQRKSLVMEIYNPYSIVQVSEVLSELSVRMGAKNAYLGKAVVMSLVNTGLTAVVSLTLIDEWRELTDVNDAPKSVAREAELFVQDWDTRFNIRRDYQIVVNEMRAYLSEVSRWVEQVDLTESLPKREGRLREDVFYELATPLMGKVKVYLDWLEDEAQRVDPELAPVHRTYAQAALHPLLLRAPFVYRTFTKPLGYAGDYEMVNQILSDPQQGPTTYFQIVNTAFLKAAVATAHRNRIDLLVDFLTRQAERARAEGRPFRVLNVGCGPAFEIQRFVREYANPELLSFQLVDFSAETLEYTRGSIERSAASAGHKVSVEMVHQSVHDLLKRRITADDPSVREFDAVYCAGLFDYLSDKVCSRLLSYFASRTRPGGHLLVTNVHSDNPEKFGMEHLLEWYLIYRDEAGMASLLPENSHAHRLYVDETGVNVFAEAAIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="96" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LJ53</accession>
    <name>Q1LJ53_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Histidine kinase</fullName>
            <ecNumber evidence="2">2.7.13.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">phcR</name>
        <name evidence="7" type="ordered locus">Rmet_2950</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.</text>
            <dbReference type="EC" id="2.7.13.3"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.7.13.3" evidence="2"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09823.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517490.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2950"/>
    <dbReference type="EnsemblBacteria" id="ABF09823">
        <property type="protein sequence ID" value="ABF09823"/>
        <property type="gene ID" value="Rmet_2950"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2950"/>
    <dbReference type="eggNOG" id="COG0784">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG4191">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_114_72_4"/>
    <dbReference type="OMA" id="PNCVALV"/>
    <dbReference type="OrthoDB" id="1755994at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00082">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003661">
        <property type="entry name" value="HisK_dim/P"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036097">
        <property type="entry name" value="HisK_dim/P_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004358">
        <property type="entry name" value="Sig_transdc_His_kin-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00512">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00344">
        <property type="entry name" value="BCTRLSENSOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00388">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47384">
        <property type="entry name" value="SSF47384"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="SSF55874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="3 4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Response regulatory" evidence="6">
        <location>
            <begin position="11"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="5">
        <location>
            <begin position="149"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="4">
        <location>
            <position position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABF09823.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="378" mass="40871" checksum="48F9B78C311A7EFA" modified="2006-05-30" version="1">MTTDTSQPRATILYVDDEEMACKYFARAAGSEHEVLSATGADEAIAILQEHQAKISVLVTDYRMPGRDGGDLLRQVALEYPQIVRILVTAYADKDMLLETVNSGEVFKILEKPITVTDVREVLRLAGERYRERAVRQQRLMAIDETLAFLAHELNTPLAAIANFARGIEARVAGEYNQQRNAEIGRAAGAMHDNAQYCLAVLSSFLQSVRSSAGGAKPKPGPGNDVSAGALVASLLDSYPFADNQRNWVQVELHDDFPVPTLPNCVALVLSSVMSNALRALADVDNPSLRFVVVAGDRPEIRIADNGPGIPPEVMDRLLVDPVTTHASAGGSGMGMIFCNRVMQSFGGGIRIASASGAGTTVTLDFPNMKNRMNRSDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LJF0</accession>
    <name>Q1LJF0_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_2853</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09726.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517411.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2853"/>
    <dbReference type="EnsemblBacteria" id="ABF09726">
        <property type="protein sequence ID" value="ABF09726"/>
        <property type="gene ID" value="Rmet_2853"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2853"/>
    <dbReference type="eggNOG" id="COG3758">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_090931_5_0_4"/>
    <dbReference type="OMA" id="DFNIMTQ"/>
    <dbReference type="OrthoDB" id="1912404at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011051">
        <property type="entry name" value="RmlC_Cupin_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010282">
        <property type="entry name" value="Uncharacterised_HutD/Ves"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37943">
        <property type="entry name" value="PTHR37943"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05962">
        <property type="entry name" value="HutD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51182">
        <property type="entry name" value="SSF51182"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF09726.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="191" mass="20687" checksum="16E27836681D9252" modified="2006-05-30" version="1">MKFGLDRIVPTPWKNGGGTTREIAVWPPGAGMDTFDWRISVADIAMDGPFSAFPGIDRQIVLIDGAGVHLQAHDDSFCHKLRHLGEPFAFSGDSAVHATLVDGPTRDFNVMTRRGVCRAEVRLMRQAFTADPTDATVLILVLRGAWDCGDGQHMTAGDGIVMVPGAPAVTMIHDEMSALCLRVALHKEPVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="92" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LJJ0</accession>
    <name>Q1LJJ0_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Sulfate adenylyltransferase subunit 2</fullName>
            <ecNumber evidence="2">2.7.7.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">ATP-sulfurylase small subunit</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="5">Sulfate adenylate transferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">cysD</name>
        <name evidence="8" type="ordered locus">Rmet_2813</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PAPS reductase family. CysD subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.4" evidence="2"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09686.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2813"/>
    <dbReference type="EnsemblBacteria" id="ABF09686">
        <property type="protein sequence ID" value="ABF09686"/>
        <property type="gene ID" value="Rmet_2813"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2813"/>
    <dbReference type="eggNOG" id="COG0175">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_043026_0_0_4"/>
    <dbReference type="OMA" id="LMIDTGH"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004781">
        <property type="term" value="F:sulfate adenylyltransferase (ATP) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019419">
        <property type="term" value="P:sulfate reduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01713">
        <property type="entry name" value="PAPS_reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002500">
        <property type="entry name" value="PAPS_reduct"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011784">
        <property type="entry name" value="SO4_adenylTrfase_ssu"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01507">
        <property type="entry name" value="PAPS_reduct"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002936">
        <property type="entry name" value="CysDAde_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PAPS_reduct" evidence="7">
        <location>
            <begin position="47"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="300"/>
            <end position="323"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012391"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00030256"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00031812"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01507"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ABF09686.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="323" mass="36495" checksum="498E3DEB4CCD2435" modified="2006-05-30" version="1">MGIMNDIASAGIPANVEHLLQVQNDHLDRLEAESIYIIREVVAECRNPALLFSGGKDSIVMLHLALKAFRLGDRKVELPFPLVHIDTGHNYPEVIQFRDQRVGELGARLVVGHVEDSIKRGTVRLRKETDSRNAAQAVTLLETIEAHKFDALMGGARRDEEKARAKERIFSFRDEFGQWDPKAQRPELWSLYNARMAQGEQMRVFPISNWTELDVWQYIARENLALPPIYYAHEREVVRKNGLLVPVTPITPKQDGDVSEVLSVRFRTVGDISCTCPVASTAATPVEIIAETAVTEITERGATRMDDQTSEASMERRKKEGYF</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="113" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LJJ1</accession>
    <name>Q1LJJ1_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Sulfate adenylyltransferase</fullName>
            <ecNumber evidence="1">2.7.7.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">cysN</name>
        <name evidence="8" type="ordered locus">Rmet_2812</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.7.7.4" evidence="1"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09685.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008644107.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2812"/>
    <dbReference type="EnsemblBacteria" id="ABF09685">
        <property type="protein sequence ID" value="ABF09685"/>
        <property type="gene ID" value="Rmet_2812"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2812"/>
    <dbReference type="eggNOG" id="COG2895">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007265_5_2_4"/>
    <dbReference type="OMA" id="PGHVEYT"/>
    <dbReference type="OrthoDB" id="244339at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004781">
        <property type="term" value="F:sulfate adenylyltransferase (ATP) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006790">
        <property type="term" value="P:sulfur compound metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04166">
        <property type="entry name" value="CysN_ATPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03695">
        <property type="entry name" value="CysN_NodQ_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04095">
        <property type="entry name" value="CysN_NoDQ_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041757">
        <property type="entry name" value="CysN_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044138">
        <property type="entry name" value="CysN_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044139">
        <property type="entry name" value="CysN_NoDQ_III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031157">
        <property type="entry name" value="G_TR_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011779">
        <property type="entry name" value="SO4_adenylTrfase_lsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000795">
        <property type="entry name" value="T_Tr_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009001">
        <property type="entry name" value="Transl_elong_EF1A/Init_IF2_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00009">
        <property type="entry name" value="GTP_EFTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00315">
        <property type="entry name" value="ELONGATNFCT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="SSF50447"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50465">
        <property type="entry name" value="SSF50465"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02034">
        <property type="entry name" value="CysN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00301">
        <property type="entry name" value="G_TR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51722">
        <property type="entry name" value="G_TR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3 8" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Tr-type G" evidence="7">
        <location>
            <begin position="8"/>
            <end position="232"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012391"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ABF09685.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="434" mass="47005" checksum="8715B9CE905FEFF4" modified="2006-05-30" version="1">MSHQSQHQGLLRFITAGSVDDGKSTLIGRLLFDSKAVLSDQLTALANAKNKRTAGEQIDFSLLTDGLEAEREQGITIDVAYRYFSTARRKFIIADTPGHEQYTRNMVTGASTAHAAIVLVDATRVTVKDGRAELLAQTKRHSAILKLLELQHVIVAVNKMDLVDYSEQRFNEIRAAYIELAAQLGLKDVTYVPVSALRGDNIVHASDAMPWYQGEPLLPLLESLPVEDVAPVGDAALRFPVQLVVRQDGSAADDFRGYAGRVEAGTVRVGQKLRVLPANREAVVAEVLTPNGSADSASAGDTVTVRLAEDVDVSRGDMFVSADTAAESAKKLTADLCWFDDESLNPSRKYVLKHTTASVFARVSAVDRVLDVHTLSHETGRHEINLNDIGSVQISLQKPIVCDTYGDNPATGAFVLIDEATNHTVAAGMIRAYA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="69" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LJJ4</accession>
    <name>Q1LJJ4_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Predicted permease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">yjgQ</name>
        <name evidence="7" type="ordered locus">Rmet_2809</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09682.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517378.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2809"/>
    <dbReference type="DNASU" id="4039636"/>
    <dbReference type="EnsemblBacteria" id="ABF09682">
        <property type="protein sequence ID" value="ABF09682"/>
        <property type="gene ID" value="Rmet_2809"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2809"/>
    <dbReference type="eggNOG" id="COG0795">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028799_1_1_4"/>
    <dbReference type="OMA" id="WFTSKLA"/>
    <dbReference type="OrthoDB" id="1558251at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043190">
        <property type="term" value="C:ATP-binding cassette (ABC) transporter complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030923">
        <property type="entry name" value="LptG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005495">
        <property type="entry name" value="LptG/LptF_permease"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33529">
        <property type="entry name" value="PTHR33529"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03739">
        <property type="entry name" value="LptF_LptG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR04408">
        <property type="entry name" value="LptG_lptG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="63"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="101"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="304"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="330"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="363"/>
            <end position="384"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABF09682.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="387" mass="43819" checksum="0B54920AEB595B8F" modified="2006-05-30" version="1">MRVLSVYERYFARLIYGVFAFILFAVLALFVFFDMLSELESVQGKYTSLIAFFHVMLEAPTRIYEVLPIAVLISAIYVFSQLASQSEYTIFRVAGLNTRQALFSLFKLAVPLALATFIFGEFIGPAAEQYAQKIKLEAIGATVSSGFRSGVWVKDRDREPEEKGAGEITRFVNVQGLRPDQSITGVTIYEFDKDYRLRVIRVAKEGKYQGGQSWQLNDVNETRFIELRDGQPAANRDALAPDFRGEQVQFPNILMHSELTPQILSVLLVQPENMSTVNLFRYIRHLRDNKQDTQRYEIAFWKKVVYPLTLFVMVALALPFAYLHARAGAVGVKVFGGIMLGLSFHLSNTLFSHVGLLHTWPPIVSALVPGTLYLMIALGALRWVDRH</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-01-15" modified="2022-08-03" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LJP8</accession>
    <name>DCD_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">dCTP deaminase</fullName>
            <ecNumber evidence="1">3.5.4.13</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Deoxycytidine triphosphate deaminase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">dcd</name>
        <name type="ordered locus">Rmet_2755</name>
    </gene>
    <organism>
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the deamination of dCTP to dUTP.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>dCTP + H(+) + H2O = dUTP + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:22680"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:61481"/>
            <dbReference type="ChEBI" id="CHEBI:61555"/>
            <dbReference type="EC" id="3.5.4.13"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Pyrimidine metabolism; dUMP biosynthesis; dUMP from dCTP (dUTP route): step 1/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homotrimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the dCTP deaminase family.</text>
    </comment>
    <dbReference type="EC" id="3.5.4.13" evidence="1"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09628.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008646296.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q1LJP8"/>
    <dbReference type="SMR" id="Q1LJP8"/>
    <dbReference type="STRING" id="266264.Rmet_2755"/>
    <dbReference type="EnsemblBacteria" id="ABF09628">
        <property type="protein sequence ID" value="ABF09628"/>
        <property type="gene ID" value="Rmet_2755"/>
    </dbReference>
    <dbReference type="GeneID" id="60820795"/>
    <dbReference type="KEGG" id="rme:Rmet_2755"/>
    <dbReference type="eggNOG" id="COG0717">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_087476_4_0_4"/>
    <dbReference type="OMA" id="FENHRYP"/>
    <dbReference type="OrthoDB" id="1598407at2"/>
    <dbReference type="UniPathway" id="UPA00610">
        <property type="reaction ID" value="UER00665"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008829">
        <property type="term" value="F:dCTP deaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006226">
        <property type="term" value="P:dUMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006229">
        <property type="term" value="P:dUTP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd07557">
        <property type="entry name" value="trimeric_dUTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00146">
        <property type="entry name" value="dCTP_deaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011962">
        <property type="entry name" value="dCTP_deaminase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029054">
        <property type="entry name" value="dUTPase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036157">
        <property type="entry name" value="dUTPase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033704">
        <property type="entry name" value="dUTPase_trimeric"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00692">
        <property type="entry name" value="dUTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51283">
        <property type="entry name" value="SSF51283"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02274">
        <property type="entry name" value="dCTP_deam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0546">Nucleotide metabolism</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_1000009795" description="dCTP deaminase">
        <location>
            <begin position="1"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="1">
        <location>
            <position position="137"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="111"/>
            <end position="116"/>
        </location>
        <ligand>
            <name>dCTP</name>
            <dbReference type="ChEBI" id="CHEBI:61481"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="135"/>
            <end position="137"/>
        </location>
        <ligand>
            <name>dCTP</name>
            <dbReference type="ChEBI" id="CHEBI:61481"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="156"/>
        </location>
        <ligand>
            <name>dCTP</name>
            <dbReference type="ChEBI" id="CHEBI:61481"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="170"/>
        </location>
        <ligand>
            <name>dCTP</name>
            <dbReference type="ChEBI" id="CHEBI:61481"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="180"/>
        </location>
        <ligand>
            <name>dCTP</name>
            <dbReference type="ChEBI" id="CHEBI:61481"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00146"/>
        </source>
    </evidence>
    <sequence length="188" mass="21209" checksum="E3B65CD4905728B7" modified="2006-05-30" version="1">MSIKSDKWIRRMAEQHGMIEPFEPGQVRESDGRKIVSYGTSSYGYDIRCADEFKIFTNINSTIVDPKNFDEKSFVDFKGDVCIIPPNSFALARTMEYFRIPRSVLTICLGKSTYARCGIIVNVTPFEPEWEGYVTLEFSNTTPLPAKIYAGEGCAQVLFFESDEICETSYADRGGKYQGQQGVTLPKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LJR8</accession>
    <name>Q1LJR8_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 5">dTDP-glucose 4,6-dehydratase</fullName>
            <ecNumber evidence="4 5">4.2.1.46</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">rfbB</name>
        <name evidence="7" type="ordered locus">Rmet_2735</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 5">
            <text>dTDP-alpha-D-glucose = dTDP-4-dehydro-6-deoxy-alpha-D-glucose + H2O</text>
            <dbReference type="Rhea" id="RHEA:17221"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:57477"/>
            <dbReference type="ChEBI" id="CHEBI:57649"/>
            <dbReference type="EC" id="4.2.1.46"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 5">
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3 5">Belongs to the NAD(P)-dependent epimerase/dehydratase family. dTDP-glucose dehydratase subfamily.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.46" evidence="4 5"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09608.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517309.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2735"/>
    <dbReference type="EnsemblBacteria" id="ABF09608">
        <property type="protein sequence ID" value="ABF09608"/>
        <property type="gene ID" value="Rmet_2735"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2735"/>
    <dbReference type="eggNOG" id="COG1088">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007383_1_14_4"/>
    <dbReference type="OMA" id="KLIPLMC"/>
    <dbReference type="OrthoDB" id="707977at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008460">
        <property type="term" value="F:dTDP-glucose 4,6-dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009225">
        <property type="term" value="P:nucleotide-sugar metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05246">
        <property type="entry name" value="dTDP_GD_SDR_e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005888">
        <property type="entry name" value="dTDP_Gluc_deHydtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016040">
        <property type="entry name" value="NAD(P)-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16363">
        <property type="entry name" value="GDP_Man_Dehyd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01181">
        <property type="entry name" value="dTDP_gluc_dehyt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0456">Lyase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAD(P)-bd_dom" evidence="6">
        <location>
            <begin position="5"/>
            <end position="325"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001539"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001911"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU004473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF16363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABF09608.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="354" mass="39323" checksum="CBC97D58B8762C6D" modified="2006-05-30" version="1">MSHILVTGGAGFIGANFVLNWLAQPGADSVVNVDKLTYAGNRKTLASLDGDSRHVFSQTDICDRDALDRLLAEHKPRAIVHFAAESHVDRSIHGPAEFIQTNIVGTFTLLEAVRAYWNGLDAEAKAAFRFLHVSTDEVFGSLGPADPQFSETTAYAPNSPYSASKAASDHLVRAYHHTYGLPVLTTNCSNNYGPYHFPEKLIPLMITNALSGKPLPVYGDGQNVRDWLYVGDHCSAIREVLARGRVGETYNVGGWNEMTNLDVVHTICSLLDQLKPKAAGSYSDQINFVKDRPGHDRRYAIDARKLERELGWKPAETFESGLRKTVQWYLDNQPWVQDVISGEYRNWVAKQYAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="69" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LJU0</accession>
    <name>Q1LJU0_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Putative heat shock protein (HslJ-like)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Rmet_2713</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09586.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517285.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2713"/>
    <dbReference type="EnsemblBacteria" id="ABF09586">
        <property type="protein sequence ID" value="ABF09586"/>
        <property type="gene ID" value="Rmet_2713"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2713"/>
    <dbReference type="eggNOG" id="COG3187">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_063818_1_0_4"/>
    <dbReference type="OMA" id="QFVEREG"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.128.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005184">
        <property type="entry name" value="DUF306_Meta_HslJ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025485">
        <property type="entry name" value="DUF4377"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038670">
        <property type="entry name" value="HslJ-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14302">
        <property type="entry name" value="DUF4377"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03724">
        <property type="entry name" value="META"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0346">Stress response</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193522" evidence="2">
        <location>
            <begin position="25"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="domain" description="META" evidence="3">
        <location>
            <begin position="47"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="domain" description="DUF4377" evidence="4">
        <location>
            <begin position="191"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="24"/>
            <end position="47"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF03724"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF14302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABF09586.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="269" mass="29418" checksum="73D8C4DE3B0EDF7B" modified="2006-05-30" version="1">MQKILRPLAMILALASATIMSACASGQPPDTPSANTSLSETRSSGPSRWELVRWQRPDGTLRDIPHGDNGQPIIFDFNDGIDSPQGTVSGTSGCNRFTGGYGKTSTGIRFDRIAGTRMACPGPRMELESALLKAMSSPFTTVGTQPSAGSMGRQIVWKTADGDLLQFVEREGVGRRGAKIDAASGKEKIVYIDSQRVECSGVGKMQCYRWRESPDAPWQLWYGAIEGLDFEPGVSYKLRVREYQVPNPPADASSIRWQLLDVEERSRAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LK08</accession>
    <name>Q1LK08_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Putative major facilitator superfamily (MFS_1) transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Rmet_2641</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09518.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517217.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2641"/>
    <dbReference type="EnsemblBacteria" id="ABF09518">
        <property type="protein sequence ID" value="ABF09518"/>
        <property type="gene ID" value="Rmet_2641"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2641"/>
    <dbReference type="eggNOG" id="COG2814">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_053107_1_0_4"/>
    <dbReference type="OMA" id="DRNFGHF"/>
    <dbReference type="OrthoDB" id="626204at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001958">
        <property type="entry name" value="Tet-R_TetA/multi-R_MdtG"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01035">
        <property type="entry name" value="TCRTETA"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="SSF103473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="12"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="43"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="74"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="100"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="135"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="163"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="251"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="302"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="364"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="domain" description="MFS" evidence="6">
        <location>
            <begin position="1"/>
            <end position="390"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABF09518.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="396" mass="42093" checksum="5443F94D8F7C2384" modified="2006-05-30" version="1">MTLPHAAVPPISWLIALTVCNHLAFNASRVVVSLFAISLKASTVTLGILMSLYALLPMFLAIRAGKRIDEIGPRLPMTAGSLMVVAGTLLPAVWHDIASLYGACALIGVGFMLVQVAMQLLIGQVSTNETRLRNYTLHALGLSISGTLGPVAMGYMIEHAGYRAAFCVLVAIALVGQAGLQYVRPRLPAQGGNAGRIAIEDGSRRSTLDLLQHPELRAVFIASAVLSAAWDLHAFLIPIQGTHIGLSPSSIGWVLGAFSIATLAIRVAMPMVSRHFSEWRIIRLALTVGAMAYLVYPFVSHFWLMCSLAFVLGLALGSAQPNVMNILHTVSPSGRAGEALGLRSAVLNTSQVVWPLTFGVVGTALGMLPIFLSMAGAMGMAGYYSRKQSRKIALPD</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-10-31" modified="2022-08-03" version="97" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LK34</accession>
    <name>DXS_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">1-deoxy-D-xylulose-5-phosphate synthase</fullName>
            <ecNumber evidence="1">2.2.1.7</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">1-deoxyxylulose-5-phosphate synthase</fullName>
            <shortName evidence="1">DXP synthase</shortName>
            <shortName evidence="1">DXPS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">dxs</name>
        <name type="ordered locus">Rmet_2615</name>
    </gene>
    <organism>
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the acyloin condensation reaction between C atoms 2 and 3 of pyruvate and glyceraldehyde 3-phosphate to yield 1-deoxy-D-xylulose-5-phosphate (DXP).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>D-glyceraldehyde 3-phosphate + H(+) + pyruvate = 1-deoxy-D-xylulose 5-phosphate + CO2</text>
            <dbReference type="Rhea" id="RHEA:12605"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57792"/>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
            <dbReference type="EC" id="2.2.1.7"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="1">Binds 1 Mg(2+) ion per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>thiamine diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58937"/>
        </cofactor>
        <text evidence="1">Binds 1 thiamine pyrophosphate per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Metabolic intermediate biosynthesis; 1-deoxy-D-xylulose 5-phosphate biosynthesis; 1-deoxy-D-xylulose 5-phosphate from D-glyceraldehyde 3-phosphate and pyruvate: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the transketolase family. DXPS subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.2.1.7" evidence="1"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09492.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517195.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q1LK34"/>
    <dbReference type="SMR" id="Q1LK34"/>
    <dbReference type="STRING" id="266264.Rmet_2615"/>
    <dbReference type="EnsemblBacteria" id="ABF09492">
        <property type="protein sequence ID" value="ABF09492"/>
        <property type="gene ID" value="Rmet_2615"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2615"/>
    <dbReference type="eggNOG" id="COG1154">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009227_1_4_4"/>
    <dbReference type="OMA" id="QVGYHAQ"/>
    <dbReference type="OrthoDB" id="566385at2"/>
    <dbReference type="UniPathway" id="UPA00064">
        <property type="reaction ID" value="UER00091"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008661">
        <property type="term" value="F:1-deoxy-D-xylulose-5-phosphate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030976">
        <property type="term" value="F:thiamine pyrophosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052865">
        <property type="term" value="P:1-deoxy-D-xylulose 5-phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016114">
        <property type="term" value="P:terpenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009228">
        <property type="term" value="P:thiamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02007">
        <property type="entry name" value="TPP_DXS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00315">
        <property type="entry name" value="DXP_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005477">
        <property type="entry name" value="Dxylulose-5-P_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009014">
        <property type="entry name" value="Transketo_C/PFOR_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005475">
        <property type="entry name" value="Transketolase-like_Pyr-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020826">
        <property type="entry name" value="Transketolase_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033248">
        <property type="entry name" value="Transketolase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005474">
        <property type="entry name" value="Transketolase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43322">
        <property type="entry name" value="PTHR43322"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13292">
        <property type="entry name" value="DXP_synthase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02779">
        <property type="entry name" value="Transket_pyr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02780">
        <property type="entry name" value="Transketolase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00861">
        <property type="entry name" value="Transket_pyr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="SSF52518"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52922">
        <property type="entry name" value="SSF52922"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00204">
        <property type="entry name" value="dxs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00801">
        <property type="entry name" value="TRANSKETOLASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00802">
        <property type="entry name" value="TRANSKETOLASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0414">Isoprene biosynthesis</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0784">Thiamine biosynthesis</keyword>
    <keyword id="KW-0786">Thiamine pyrophosphate</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000256466" description="1-deoxy-D-xylulose-5-phosphate synthase">
        <location>
            <begin position="1"/>
            <end position="637"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="75"/>
        </location>
        <ligand>
            <name>thiamine diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58937"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="116"/>
            <end position="118"/>
        </location>
        <ligand>
            <name>thiamine diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58937"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="147"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="148"/>
            <end position="149"/>
        </location>
        <ligand>
            <name>thiamine diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58937"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="177"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="177"/>
        </location>
        <ligand>
            <name>thiamine diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58937"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="288"/>
        </location>
        <ligand>
            <name>thiamine diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58937"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="370"/>
        </location>
        <ligand>
            <name>thiamine diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58937"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00315"/>
        </source>
    </evidence>
    <sequence length="637" mass="68870" checksum="79CD97C0C3A948A4" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LK46</accession>
    <name>Q1LK46_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Enoyl-CoA hydratase/isomerase</fullName>
            <ecNumber evidence="4">4.2.1.17</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">paaG1</name>
        <name evidence="4" type="ordered locus">Rmet_2603</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the enoyl-CoA hydratase/isomerase family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.17" evidence="4"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09480.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517183.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2603"/>
    <dbReference type="EnsemblBacteria" id="ABF09480">
        <property type="protein sequence ID" value="ABF09480"/>
        <property type="gene ID" value="Rmet_2603"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2603"/>
    <dbReference type="eggNOG" id="COG1024">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009834_7_6_4"/>
    <dbReference type="OMA" id="AMEMIMT"/>
    <dbReference type="OrthoDB" id="1498685at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004300">
        <property type="term" value="F:enoyl-CoA hydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016853">
        <property type="term" value="F:isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.12.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029045">
        <property type="entry name" value="ClpP/crotonase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018376">
        <property type="entry name" value="Enoyl-CoA_hyd/isom_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001753">
        <property type="entry name" value="Enoyl-CoA_hydra/iso"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014748">
        <property type="entry name" value="Enoyl-CoA_hydra_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00378">
        <property type="entry name" value="ECH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52096">
        <property type="entry name" value="SSF52096"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00166">
        <property type="entry name" value="ENOYL_COA_HYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0413">Isomerase</keyword>
    <keyword evidence="4" id="KW-0456">Lyase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF09480.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="263" mass="28683" checksum="C4AE8CBA1BB2B078" modified="2006-05-30" version="1">MQFETLLYEVVDGVAIIRLNRPDRMNTLGGTMKPDLARAFFELARDDTSVRCVVLTGTGDRAFCAGADIKERAGGTAHPADYYVTQQRTHELFRGIEEFEKPVIAAINGVALGGGLELALCADIRLAARSARFGLPEIKLGVIPAAGGTQRLPRVVGPAIAKELIFTSELINADAAKDIRLVNRVIDDDKLMDAALTMARRIAAQPPLAVRFAKRSVNLGMQGSVDMGLQFECYGAAMVMDSEDRKEGMRAFVEKREPRFVGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LKB1</accession>
    <name>Q1LKB1_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Outer membrane protein (Porin)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Rmet_2538</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09415.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517115.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2538"/>
    <dbReference type="EnsemblBacteria" id="ABF09415">
        <property type="protein sequence ID" value="ABF09415"/>
        <property type="gene ID" value="Rmet_2538"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2538"/>
    <dbReference type="eggNOG" id="COG3203">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038238_2_0_4"/>
    <dbReference type="OMA" id="TAFAFKY"/>
    <dbReference type="OrthoDB" id="1064531at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046930">
        <property type="term" value="C:pore complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015288">
        <property type="term" value="F:porin activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006811">
        <property type="term" value="P:ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00342">
        <property type="entry name" value="gram_neg_porins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.160.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033900">
        <property type="entry name" value="Gram_neg_porin_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023614">
        <property type="entry name" value="Porin_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13609">
        <property type="entry name" value="Porin_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0406">Ion transport</keyword>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0626">Porin</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1134">Transmembrane beta strand</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193625" evidence="4">
        <location>
            <begin position="21"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="domain" description="Porin_4" evidence="5">
        <location>
            <begin position="8"/>
            <end position="347"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF13609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABF09415.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="382" mass="40915" checksum="14C410343E050504" modified="2006-05-30" version="1">MKRKFLTLAALAPAAVTAHAQTSVTLYGVIDGSVEYVNRVAAGAATPTQGGSRFGMPSVGGLSASRWGLRGVEDLGAGNSAMFVLESGFQWDSGALQSAPLFNRQSYIGLQNKEYGKLSFGRQYTSFFDGMANFAPLRFAATYEPGIWWMGLNYREDNMVKYTGQFGPLQAVGHFSFGTGTAVQNANIVLANGGVGETPGNFTDNTAYGGSLMYLDSSGLGFSVAADIWRPAAVTGQPGRVNKYGAAALYNTGPFKFTAGYRYNKGEFSNGATVLRDDYWWAGINYQATPALGLSLAYYYADIKSARTAATSPETNPANMQQVSFIADYNFSKRTDVYLSVGYAHNGGLSFDSTATAFAFKYPTMTGQQNMVGVTVGVRQVF</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-02-05" modified="2022-05-25" version="95" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LKM2</accession>
    <name>ACP_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Acyl carrier protein</fullName>
            <shortName evidence="1">ACP</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">acpP</name>
        <name type="ordered locus">Rmet_2427</name>
    </gene>
    <organism>
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Carrier of the growing fatty acid chain in fatty acid biosynthesis.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Lipid metabolism; fatty acid biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="PTM">
        <text evidence="1">4'-phosphopantetheine is transferred from CoA to a specific serine of apo-ACP by AcpS. This modification is essential for activity because fatty acids are bound in thioester linkage to the sulfhydryl of the prosthetic group.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the acyl carrier protein (ACP) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09304.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008644813.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q1LKM2"/>
    <dbReference type="SMR" id="Q1LKM2"/>
    <dbReference type="STRING" id="266264.Rmet_2427"/>
    <dbReference type="EnsemblBacteria" id="ABF09304">
        <property type="protein sequence ID" value="ABF09304"/>
        <property type="gene ID" value="Rmet_2427"/>
    </dbReference>
    <dbReference type="GeneID" id="60821099"/>
    <dbReference type="KEGG" id="rme:Rmet_2427"/>
    <dbReference type="eggNOG" id="COG0236">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_108696_5_1_4"/>
    <dbReference type="OMA" id="CEIPDEQ"/>
    <dbReference type="OrthoDB" id="1943389at2"/>
    <dbReference type="UniPathway" id="UPA00094"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000036">
        <property type="term" value="F:acyl carrier activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1200.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01217">
        <property type="entry name" value="Acyl_carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036736">
        <property type="entry name" value="ACP-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003231">
        <property type="entry name" value="Acyl_carrier"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009081">
        <property type="entry name" value="PP-bd_ACP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006162">
        <property type="entry name" value="Ppantetheine_attach_site"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20863">
        <property type="entry name" value="PTHR20863"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00550">
        <property type="entry name" value="PP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47336">
        <property type="entry name" value="SSF47336"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00517">
        <property type="entry name" value="acyl_carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50075">
        <property type="entry name" value="CARRIER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00012">
        <property type="entry name" value="PHOSPHOPANTETHEINE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword id="KW-0276">Fatty acid metabolism</keyword>
    <keyword id="KW-0444">Lipid biosynthesis</keyword>
    <keyword id="KW-0443">Lipid metabolism</keyword>
    <keyword id="KW-0596">Phosphopantetheine</keyword>
    <keyword id="KW-0597">Phosphoprotein</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_1000066668" description="Acyl carrier protein">
        <location>
            <begin position="1"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="domain" description="Carrier" evidence="2">
        <location>
            <begin position="2"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="modified residue" description="O-(pantetheine 4'-phosphoryl)serine" evidence="2">
        <location>
            <position position="37"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00258"/>
        </source>
    </evidence>
    <sequence length="79" mass="8682" checksum="F2737B0EF58D427B" modified="2006-05-30" version="1">MDNIEQRVKKIVAEQLGVAEADIKNESSFVNDLGADSLDTVELVMALEDEFGMEIPDEEAEKITTVQQAIDYATAHVKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="55" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LL01</accession>
    <name>Q1LL01_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Integrase regulator R</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">inrR</name>
        <name evidence="1" type="ordered locus">Rmet_2296</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09175.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_003098978.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2296"/>
    <dbReference type="EnsemblBacteria" id="ABF09175">
        <property type="protein sequence ID" value="ABF09175"/>
        <property type="gene ID" value="Rmet_2296"/>
    </dbReference>
    <dbReference type="GeneID" id="56409805"/>
    <dbReference type="GeneID" id="61526424"/>
    <dbReference type="KEGG" id="rme:Rmet_2296"/>
    <dbReference type="eggNOG" id="ENOG502Z824">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_129290_1_0_4"/>
    <dbReference type="OMA" id="WASECHA"/>
    <dbReference type="OrthoDB" id="1663131at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021502">
        <property type="entry name" value="DUF3158"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11358">
        <property type="entry name" value="DUF3158"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF09175.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="175" mass="19840" checksum="9FB1DEF30E4CF142" modified="2006-05-30" version="1">MSDPNREPRYFRRLEQPAFMRLEHAASLKGLLKPFKGKGDFEAWASQCFAMRDELIALAQRQVLPQACGHPFHLLPVELAQQTTGAGTTFLRWRKHDRSAMGVALWQELMASPSTPVNLLHDLHEIELQRVMLNMQISLLHTLGRQAQECASKAAQADNTYLRRLASVPAAVRDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="60" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LL37</accession>
    <name>Q1LL37_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_2260</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09139.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516962.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2260"/>
    <dbReference type="EnsemblBacteria" id="ABF09139">
        <property type="protein sequence ID" value="ABF09139"/>
        <property type="gene ID" value="Rmet_2260"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2260"/>
    <dbReference type="eggNOG" id="ENOG502ZAGN">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_117549_0_0_4"/>
    <dbReference type="OMA" id="YYVGYFS"/>
    <dbReference type="OrthoDB" id="1616869at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046239">
        <property type="entry name" value="DUF6272"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19788">
        <property type="entry name" value="DUF6272"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF09139.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="190" mass="21104" checksum="744A4B8179CC7FCC" modified="2006-05-30" version="1">MTAAQSIDNEYEPFFAMARERNVIFYYVGYFSQNIIAAMSDAVRLQLSVSGVDGTTRRKLFSSFVEMAQNIVHYSSDSLTSPSQADGHLRHGSVCISMVDEHYRLMCSNPVSTARVAQLRGTLERVRTMTLDEIKRAYKETLRAEVPEGSKGAGLGILTMARDASAPLEYNFQPIDSDPVSTMFQLVATI</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LLA3</accession>
    <name>Q1LLA3_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Putative neutral amino-acid efflux system</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">yeaS</name>
        <name evidence="6" type="ordered locus">Rmet_2194</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09073.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516901.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2194"/>
    <dbReference type="EnsemblBacteria" id="ABF09073">
        <property type="protein sequence ID" value="ABF09073"/>
        <property type="gene ID" value="Rmet_2194"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2194"/>
    <dbReference type="eggNOG" id="COG1280">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_079569_3_1_4"/>
    <dbReference type="OMA" id="HTWISYL"/>
    <dbReference type="OrthoDB" id="1927576at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006865">
        <property type="term" value="P:amino acid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001123">
        <property type="entry name" value="LeuE-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30086">
        <property type="entry name" value="PTHR30086"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01810">
        <property type="entry name" value="LysE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006324">
        <property type="entry name" value="LeuE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="14"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="51"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="79"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="130"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="163"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="198"/>
            <end position="218"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABF09073.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="223" mass="23722" checksum="A6036DC99AEE565F" modified="2006-05-30" version="1">MNAFMHANLGITDFWTFVLGTIFIVLLPGPNSMYVLSVAAQRGVRAGYKGACGVFLGDAILMVLSAAGVASLLKASPGLFHVVKYVGAAYLAWIGFQMLRGAVRNWSAQADVATNAVASLDRASNPFSKALFISLLNPKAILFFISFFIQFVDPQFVVPALSFVALGLVCQICSFAYLTTIIFVGARLADAFRRRRRLSAGMSSGVGAMFIGFGAKLATATLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="72" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LLC4</accession>
    <name>Q1LLC4_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Diguanylate cyclase (GGDEF domain protein)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Rmet_2173</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09052.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516882.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2173"/>
    <dbReference type="EnsemblBacteria" id="ABF09052">
        <property type="protein sequence ID" value="ABF09052"/>
        <property type="gene ID" value="Rmet_2173"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2173"/>
    <dbReference type="eggNOG" id="COG3706">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_11_1_4"/>
    <dbReference type="OMA" id="EPDPIAY"/>
    <dbReference type="OrthoDB" id="1635706at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01949">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000160">
        <property type="entry name" value="GGDEF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029787">
        <property type="entry name" value="Nucleotide_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00990">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00267">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55073">
        <property type="entry name" value="SSF55073"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00254">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50887">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="6"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="37"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="62"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="94"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="128"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="163"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="207"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="domain" description="GGDEF" evidence="3">
        <location>
            <begin position="266"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="394"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="400"/>
            <end position="416"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF09052.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="416" mass="44922" checksum="28D6EC3D5CEB711D" modified="2006-05-30" version="1">MFQSQVVVLIAGLIALQMAIVCAVLRRSAGMERSGLTSWALGDLLVTLAVVLQAAHAYRPEWPLAVLPEFALTAGLSMMVYGTRHFAGKRGRGVVLGVLNLPGLAALTIYAVGGLAEMGLGRVPTGTLAWLGLATASIQTLLLLEIARITVALVPLKRGTGRLAALSLSVLSLGCAVLAGAEAWPMFAALMDGHVQPLVDRHESMTVWFVFGVMGLAVSFALMAHDRLRQIIERRARHDDLTDVLSRGAFWEELELACKQAERSRKPLTVAFMDLDHFKAINDLYGHLAGDSVLRHFAGLLRKVAAPDSVIGRLGGEEFAIVMPATGLETGRAISVRLSALVRATPCPSEPDPIAYTVSIGMAEWQLGENADAVMRRSDRALYDAKQMGRNCVSSHDAEEFTGMPRRPRVREREPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-10-07" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LLE1</accession>
    <name>Q1LLE1_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_2156</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09035.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2156"/>
    <dbReference type="EnsemblBacteria" id="ABF09035">
        <property type="protein sequence ID" value="ABF09035"/>
        <property type="gene ID" value="Rmet_2156"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2156"/>
    <dbReference type="HOGENOM" id="CLU_1516697_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF09035.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="177" mass="19347" checksum="E86381FB66303FB0" modified="2006-05-30" version="1">MSGGSTMLRPHSQSGLFPEKPLREQPVLDNALNLLPVGLNQVRPGEEKFRELVVVAIKVCRRNLEHLRQLLSDFNSGLVNSTLVSADACAGNSFVQADFHAELILGNAGTPPCLPQATSKNSNRLLFSHSSNIVDFALRFPTKFVESGTNYEQPNALDKPPFHGSFVAIGVINNHWS</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="70" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LLH3</accession>
    <name>Q1LLH3_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Hydroxymethylglutaryl-CoA lyase</fullName>
            <ecNumber evidence="4">4.1.3.4</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Rmet_2124</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the HMG-CoA lyase family.</text>
    </comment>
    <dbReference type="EC" id="4.1.3.4" evidence="4"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09003.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2124"/>
    <dbReference type="EnsemblBacteria" id="ABF09003">
        <property type="protein sequence ID" value="ABF09003"/>
        <property type="gene ID" value="Rmet_2124"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2124"/>
    <dbReference type="eggNOG" id="COG0119">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022138_3_2_4"/>
    <dbReference type="OMA" id="KAGRNCD"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004419">
        <property type="term" value="F:hydroxymethylglutaryl-CoA lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043594">
        <property type="entry name" value="HMGL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000891">
        <property type="entry name" value="PYR_CT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42738">
        <property type="entry name" value="PTHR42738"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00682">
        <property type="entry name" value="HMGL-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50991">
        <property type="entry name" value="PYR_CT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0456">Lyase</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pyruvate carboxyltransferase" evidence="3">
        <location>
            <begin position="18"/>
            <end position="285"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF09003.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="326" mass="34163" checksum="4722AE030A90E8D6" modified="2006-05-30" version="1">MSAKMSAATYPLSGPAKVEINEVAPRDGLQIEPFIVPTEGKVAFVNALARCGFARIEATSFTSARAIPALADAEAVMHQVERVAGVGLTVLVPNLRGLERALSCRPDEVNLVMSSSETHNRANLRMTREQSQAQLLAMIAEAGKAGVPVNVSLSTVFGCPFEGDVSEAEVMRLAKAFADAGAAGITLCDTTGMAYPNQVAALCETFQRALPKTGLTIHLHNTRGMGLANAIAAWQTGVTRFDAAAGGLGGCPYAPGASGNVSTEELVHMFASMGVDTGVELGALLDVVAGVSDLVQRDVSSQLLRAGPRLRTHQPPAWMEEFFAKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LLP2</accession>
    <name>Q1LLP2_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Glycerol-3-phosphate transporter subunit periplasmic-binding component of ABC superfamily</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">ugpB</name>
        <name evidence="5" type="ordered locus">Rmet_2055</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the bacterial solute-binding protein 1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08934.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516766.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2055"/>
    <dbReference type="EnsemblBacteria" id="ABF08934">
        <property type="protein sequence ID" value="ABF08934"/>
        <property type="gene ID" value="Rmet_2055"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2055"/>
    <dbReference type="eggNOG" id="COG1653">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031285_3_0_4"/>
    <dbReference type="OMA" id="ESFSAWH"/>
    <dbReference type="OrthoDB" id="447734at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006059">
        <property type="entry name" value="SBP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006061">
        <property type="entry name" value="SBP_1_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13416">
        <property type="entry name" value="SBP_bac_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01037">
        <property type="entry name" value="SBP_BACTERIAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193654" evidence="4">
        <location>
            <begin position="25"/>
            <end position="436"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABF08934.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="436" mass="47793" checksum="82FCB846571D6981" modified="2006-05-30" version="1">MSFPRTALKFAAVASLALAGTAHAAVEIQWWHAMQGALNDKVNEIADKFNASQSDYKIVPVNKGNYDETMAAGIAAFRAGGAPAILQVFEVGTATMMSAKGAIKPVSQVMKDAGEKFDQKAYIPAVAGYYTSSKGEMLSFPFNSSTTVFYYNKDAFKKAGISAPPKTWPEVMQYSAKLKASGQNCAYTTDWQSWVHLESFSAWHNTLFATKNNGFGGTDARLVFNSPLHVKHITNLQEMVKKGYFSYGGRKAESQAKFYNGECAMFTGSSASLANIRKNAKFQFGVSQLPYYPDVPGAPQNTIIGGASLWVMGGKKADEYKGVAKFFTFLSRPEIQSDWHQATGYLPVTMAAYEMTRKSGYYDKNPGADVSVEQMVVKTTDKSRGVRLGNLVQIRTVIDEELEAVWAGKKEPKAALDNAVARGNELLERFQKTARE</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LLU4</accession>
    <name>Q1LLU4_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_2003</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08882.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516721.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2003"/>
    <dbReference type="EnsemblBacteria" id="ABF08882">
        <property type="protein sequence ID" value="ABF08882"/>
        <property type="gene ID" value="Rmet_2003"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2003"/>
    <dbReference type="HOGENOM" id="CLU_1359466_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF08882.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="201" mass="22765" checksum="B322B98CD5D0C3BE" modified="2006-05-30" version="1">MIYLDDATAAELRAGYEMELPLTPVGEVDKFLADFVDATEFIGLTVFEQTQEKRRGAFDKAATALMNLLEALSGLSDIDNSQLRFLLTVEIESRLPESLRLQWQEIVSSLTKTDALAALGQLDLHARGMAQVAQGGERLEAFAGRMLLRVFRKHHLPWDYAETGYAATCLRVMLRLSGRDDITEQKRIRHWLEKAEQPRSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="65" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LM01</accession>
    <name>Q1LM01_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_1946</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08825.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1946"/>
    <dbReference type="EnsemblBacteria" id="ABF08825">
        <property type="protein sequence ID" value="ABF08825"/>
        <property type="gene ID" value="Rmet_1946"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1946"/>
    <dbReference type="HOGENOM" id="CLU_2104938_0_0_4"/>
    <dbReference type="OMA" id="GHPQHGI"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="64"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="64"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="87"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="117"/>
            <end position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF08825.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="141" mass="15421" checksum="D585C2B830013A9D" modified="2006-05-30" version="1">MLDGRRSGCRRNRHYPMKVKRIRPGHTLRRATYARAACLAGLTLLITAASGVALAQSAGMAHLLHTSKSQSSSSAQGGNGQASERGRPSGRRSDKAESERDMADRATRQGQRGGNPRLSPDERKTLRKNLYDLSREMYQGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="66" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LME9</accession>
    <name>Q1LME9_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_1798</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the AHA1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08677.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516527.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1798"/>
    <dbReference type="EnsemblBacteria" id="ABF08677">
        <property type="protein sequence ID" value="ABF08677"/>
        <property type="gene ID" value="Rmet_1798"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1798"/>
    <dbReference type="eggNOG" id="COG3832">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_899479_0_0_4"/>
    <dbReference type="OMA" id="MLGPDGW"/>
    <dbReference type="OrthoDB" id="1914312at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.530.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013538">
        <property type="entry name" value="Activator_of_Hsp90_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023393">
        <property type="entry name" value="START-like_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08327">
        <property type="entry name" value="AHSA1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006817"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF08677.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="329" mass="36486" checksum="0C7A224B84266F82" modified="2006-05-30" version="1">MATATETSKATTKTQASAAADRESLTVSRAFNAPRDLVFQAWSAAEHLKHWFCPQGFTIPECEVEFRPGGKFDLWMRSLDGNYSHWIRGHYVEIIPHSRLVLDMTVVTEAGVALFNANTSVTFETVCGGTEMSVTQRYTALDPSAGNFTRGAEEGWAQTLDRLEKEVARIAAATPARSVAHGVFTIERTYDASPELVFRALSDPVAKAQWFNGGPGYTEVKRTMDVRPGGREHLQGRWENGLVSTFDAVYFDVIPNERLVYSYEMHMGERKISVSLATFELKRAGQGTRLVMTEQGAFLDGYDDAGSREHGSGLLLDAIGEWLKAMTKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LMN4</accession>
    <name>Q1LMN4_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Multidrug resistance efflux pump</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Rmet_1711</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08592.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516444.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1711"/>
    <dbReference type="EnsemblBacteria" id="ABF08592">
        <property type="protein sequence ID" value="ABF08592"/>
        <property type="gene ID" value="Rmet_1711"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1711"/>
    <dbReference type="eggNOG" id="COG1566">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018816_15_3_4"/>
    <dbReference type="OMA" id="WYLLADR"/>
    <dbReference type="OrthoDB" id="757027at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043602">
        <property type="entry name" value="CusB_dom_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032317">
        <property type="entry name" value="HlyD_D23"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00529">
        <property type="entry name" value="CusB_dom_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16576">
        <property type="entry name" value="HlyD_D23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="29"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="domain" description="CusB_dom_1" evidence="4">
        <location>
            <begin position="61"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="domain" description="HlyD_D23" evidence="5">
        <location>
            <begin position="64"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF16576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABF08592.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="378" mass="40530" checksum="94546BC7A45E438C" modified="2006-05-30" version="1">MSDASDANAPATPPASPGPAASGDASGKGVRWVVIAIVLSLVWYLLADRFTPYTQQARVQAFVIPVASEVSGRVTQVPVHNNQNVEVGTVLFEVDPQQYRIALDRARADFESTRRQIGANTAGIDSARESLRAAQANELKARQDSERLERLYREDPGTVSVRRLEVARATHTQAVSQVGAARAEVERAKEQQGGSEENNAKLRSAAAAVEKAELDLSNAKIRARTGGLVTDLQTEVGRFAGAGSPVMTLIAIQDIWITADLTENNLGHVEPGTPVAIVLDALPGKVLEGKVRSIGYGVSTGQSPPPGSLPTVQNSRDWLRPAQRFPVIVDIDPGQLSSLRGVRVGGQAEVMAFPTSGNPLNPLGRLYIRLMSWLSYVY</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-10-07" version="69" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LMS6</accession>
    <name>Q1LMS6_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative Dipeptidyl aminopeptidase/acylaminoacyl-peptidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_1669</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08550.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1669"/>
    <dbReference type="EnsemblBacteria" id="ABF08550">
        <property type="protein sequence ID" value="ABF08550"/>
        <property type="gene ID" value="Rmet_1669"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1669"/>
    <dbReference type="eggNOG" id="COG0412">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_043246_0_0_4"/>
    <dbReference type="OMA" id="FDPEYSG"/>
    <dbReference type="OrthoDB" id="721431at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004177">
        <property type="term" value="F:aminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000383">
        <property type="entry name" value="Xaa-Pro-like_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02129">
        <property type="entry name" value="Peptidase_S15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="SSF53474"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0031">Aminopeptidase</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase_S15" evidence="1">
        <location>
            <begin position="82"/>
            <end position="223"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02129"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF08550.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="331" mass="35211" checksum="87C5D2E324FFE0C1" modified="2006-05-30" version="1">MYQEHTNDREIVVMRSARSIRTSRSACLAVIVAIACQFVADIGGSPARAAESGNDAASAKPATHAETVLIPAPGMGTFGGTVDMHADLFKPDGPGPYPVLIFSHGRAPDRLARAGLKHPIQEWHVRYWLEHGVAVLAPVRVGYGETGGPDRENNGAIFDASGRCTSKPDYANVAMALNDATLTTINWVRSQPWADGNRIVLEGRSMGGFASVAAAASRPDGVVGYINFSGGAGGSPDRSPGHSCDPDQMREVMAEFGKRVTLPGLWLYARNDRYFGAEAPLGWYAAFAAGGSPAKLISVQEVPGRDGHLLLSYGQEFWLSDLDAFVKSLGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="53" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LNB9</accession>
    <name>Q1LNB9_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_1474</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08357.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516223.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1474"/>
    <dbReference type="EnsemblBacteria" id="ABF08357">
        <property type="protein sequence ID" value="ABF08357"/>
        <property type="gene ID" value="Rmet_1474"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1474"/>
    <dbReference type="eggNOG" id="ENOG5032ZVZ">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_196766_0_0_4"/>
    <dbReference type="OMA" id="TNIADWM"/>
    <dbReference type="OrthoDB" id="2058205at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF08357.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="79" mass="9182" checksum="D3EBA7202366EC4A" modified="2006-05-30" version="1">MKFETLKNSIATLQKLRDAHYSQLDAGALAELDDVLWQLRDMSESSEKREHDHGKLLDRTLRITDIVIRAVSNLTDWMK</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="97" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LNF4</accession>
    <name>Q1LNF4_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Isoprenyl transferase</fullName>
            <ecNumber evidence="2">2.5.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">uppS</name>
        <name evidence="3" type="ordered locus">Rmet_1439</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Catalyzes the condensation of isopentenyl diphosphate (IPP) with allylic pyrophosphates generating different type of terpenoids.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="2">Binds 2 magnesium ions per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the UPP synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.-" evidence="2"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08322.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516189.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1439"/>
    <dbReference type="EnsemblBacteria" id="ABF08322">
        <property type="protein sequence ID" value="ABF08322"/>
        <property type="gene ID" value="Rmet_1439"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1439"/>
    <dbReference type="eggNOG" id="COG0020">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038505_1_1_4"/>
    <dbReference type="OMA" id="PRTEGHK"/>
    <dbReference type="OrthoDB" id="1630604at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004659">
        <property type="term" value="F:prenyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00475">
        <property type="entry name" value="Cis_IPPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1180.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01139">
        <property type="entry name" value="ISPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001441">
        <property type="entry name" value="UPP_synth-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018520">
        <property type="entry name" value="UPP_synth-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036424">
        <property type="entry name" value="UPP_synth-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10291">
        <property type="entry name" value="PTHR10291"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01255">
        <property type="entry name" value="Prenyltransf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64005">
        <property type="entry name" value="SSF64005"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00055">
        <property type="entry name" value="uppS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01066">
        <property type="entry name" value="UPP_SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0460">Magnesium</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2 3" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Substrate binding" evidence="2">
        <location>
            <begin position="26"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="2">
        <location>
            <begin position="70"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="2">
        <location>
            <begin position="197"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="active site" evidence="2">
        <location>
            <position position="25"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="2">
        <location>
            <position position="73"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="25"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="30"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="38"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="42"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="74"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="76"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="191"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="210"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF08322.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="256" mass="28898" checksum="FD6E2293B61DA9A6" modified="2006-05-30" version="1">MQHISSTLGVPDTTYVPRHVAIIMDGNGRWATQRHLPRVAGHNRGLDAVREIVQACAARGVQYLTLFAFSSENWRRPADEVSFLMRLFMTALRREVVRMHANNIRLRVVGDLSRFSPRIQQAIKDAEARTEGNTGLTVTIAANYGGRWDLLQAMRKMLARSPLLDSDAIDESVLAPHLAMAYAPEPDLFIRTGGEQRISNFLLWQLAYTELYFTETFWPDFDAAELDKAFASYRQRERRFGRTSAQLVAPGLSGTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LNP5</accession>
    <name>Q1LNP5_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Conjugal transfer trbF (Protein belongs to CMGI-2)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">trbF</name>
        <name evidence="7" type="ordered locus">Rmet_1348</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08231.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516112.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1348"/>
    <dbReference type="EnsemblBacteria" id="ABF08231">
        <property type="protein sequence ID" value="ABF08231"/>
        <property type="gene ID" value="Rmet_1348"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1348"/>
    <dbReference type="eggNOG" id="COG3701">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_076026_0_0_4"/>
    <dbReference type="OMA" id="YVIEVDQ"/>
    <dbReference type="OrthoDB" id="1214981at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd16425">
        <property type="entry name" value="TrbF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032710">
        <property type="entry name" value="NTF2-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035658">
        <property type="entry name" value="TrbF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007430">
        <property type="entry name" value="VirB8"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04335">
        <property type="entry name" value="VirB8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54427">
        <property type="entry name" value="SSF54427"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="43"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="domain" description="VirB8" evidence="6">
        <location>
            <begin position="23"/>
            <end position="225"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF04335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABF08231.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="234" mass="26121" checksum="E10030E042EFE8D9" modified="2006-05-30" version="1">MRFRKPQVRYADTPQPATPYQAAGQVWDDRIGSPRVQAKNWRLMAFGCLTLALLMAGGLVWRSAQSFVTPYVVEVDNAGQVRAVGEAATPYRPNDAQTAHHIARFVTLVRSLSIDPIVVRQNWLDAYDYTTDKGAAVLNDYARVNDPFARIGKESVTVQITSVVRASDTSFNVRWTERRYVNGAAAGLERWTAVVSIVLQPPRTEERLRKNPLGIYVNGLSWSRELDSSEGAKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LNT8</accession>
    <name>Q1LNT8_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">IS3/IS407-type transposase (OrfA)(Belongs to CMGI-2) DNA-binding protein second orf of element ares Rmet_1302 and Rmet_1279</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">tnpA</name>
        <name evidence="1" type="ordered locus">Rmet_1303</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08188.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1303"/>
    <dbReference type="EnsemblBacteria" id="ABF08188">
        <property type="protein sequence ID" value="ABF08188"/>
        <property type="gene ID" value="Rmet_1303"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1303"/>
    <dbReference type="eggNOG" id="COG2963">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027402_34_1_4"/>
    <dbReference type="OMA" id="AGMPIKE"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004803">
        <property type="term" value="F:transposase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006313">
        <property type="term" value="P:transposition, DNA-mediated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002514">
        <property type="entry name" value="Transposase_8"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01527">
        <property type="entry name" value="HTH_Tnp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF08188.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="87" mass="10185" checksum="76D0AB0FD34C6166" modified="2006-05-30" version="1">MKKRFTEEQIIGFLREAEAGMPIKELCRRHGFSEASYYLWRSKLGGMSVADAKRLKELEAENARLKKLLAESLLENEVTREALRKKW</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="72" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LNU7</accession>
    <name>Q1LNU7_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative plasmid maintenance system antidote protein, XRE family Putative HTH-type transcriptional regulator ybaQ (Belongs to CMGI-2)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_1294</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08179.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1294"/>
    <dbReference type="EnsemblBacteria" id="ABF08179">
        <property type="protein sequence ID" value="ABF08179"/>
        <property type="gene ID" value="Rmet_1294"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1294"/>
    <dbReference type="eggNOG" id="COG3093">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_140230_3_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001387">
        <property type="entry name" value="Cro/C1-type_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010982">
        <property type="entry name" value="Lambda_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013430">
        <property type="entry name" value="Toxin_antidote_HigA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36924">
        <property type="entry name" value="PTHR36924"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01381">
        <property type="entry name" value="HTH_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00530">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47413">
        <property type="entry name" value="SSF47413"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02607">
        <property type="entry name" value="antidote_HigA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50943">
        <property type="entry name" value="HTH_CROC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH cro/C1-type" evidence="1">
        <location>
            <begin position="55"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF08179.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="127" mass="13978" checksum="13B59ABF633AB7D0" modified="2006-05-30" version="1">MIKASLSFCFFIMTRIGPRSSHPAAPVLPPVGVPLRVPTHPGRLLARTCLLPLALNQSEAARLLGLSRRRLHELVHGQRAMSPDTAIRCARQFGIDAGFWLAQQAAWDSFHAWKRLSSGSATSPFPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="67" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LNW6</accession>
    <name>Q1LNW6_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_1275</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08160.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1275"/>
    <dbReference type="EnsemblBacteria" id="ABF08160">
        <property type="protein sequence ID" value="ABF08160"/>
        <property type="gene ID" value="Rmet_1275"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1275"/>
    <dbReference type="eggNOG" id="COG3436">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_128110_0_1_4"/>
    <dbReference type="OMA" id="QITPQHR"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008878">
        <property type="entry name" value="Transposase_IS66_Orf2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36455">
        <property type="entry name" value="PTHR36455"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05717">
        <property type="entry name" value="TnpB_IS66"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF08160.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="119" mass="13948" checksum="DCCCADBEB43D3E92" modified="2006-05-30" version="1">MHPGSRLGSVYLCREPVDFRKSIGGLSALVEQDLGMSPFGDALFVFVNRQRDKIKALYWHRNGFCLWYKRLERQRFAWPALAPGQRTCTLNLQELEWLLEGFDLWSHQPHKTLHYEAVT</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LNX8</accession>
    <name>Q1LNX8_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">IstB-like ATP binding protein ISRme20</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">tnpB</name>
        <name evidence="2" type="ordered locus">Rmet_1263</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08148.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516034.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1263"/>
    <dbReference type="EnsemblBacteria" id="ABF08148">
        <property type="protein sequence ID" value="ABF08148"/>
        <property type="gene ID" value="Rmet_1263"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1263"/>
    <dbReference type="eggNOG" id="COG1484">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_062999_1_0_4"/>
    <dbReference type="OMA" id="YLPMDRE"/>
    <dbReference type="OrthoDB" id="1792718at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028350">
        <property type="entry name" value="DNAC/ATP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002611">
        <property type="entry name" value="IstB_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01695">
        <property type="entry name" value="IstB_IS21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF003073">
        <property type="entry name" value="DNAC_TnpB_IstB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA" evidence="1">
        <location>
            <begin position="100"/>
            <end position="237"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF08148.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="268" mass="29893" checksum="CEFFDC3DF2170C83" modified="2006-05-30" version="1">MDIQHERILEACRQLRIDTMGEQYSMLAARAAANQLTFADFFEALLKSAMDAKHTRTKSMLLRTAGFPVLKTLEEFDFTFAHGVAKKSVLELSSMAFVERAENIVLLGPSGLGKTHLAIALGYIATQMGIKTRFISAADLVIALAAASRQDRLADFLKRNIMNPRLLIIDEVGYLPLGRDDANLFFQVIAKRYEKGSVIVTSNLPFGQWDQTFAGDQTLTAALLDRLLHHSHVLQIKGESYRLKDKRKAGVIRSRASESAEQQLEMHA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LP65</accession>
    <name>Q1LP65_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Transcriptional regulator, LysR family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_1175</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the LysR transcriptional regulatory family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08061.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515955.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1175"/>
    <dbReference type="EnsemblBacteria" id="ABF08061">
        <property type="protein sequence ID" value="ABF08061"/>
        <property type="gene ID" value="Rmet_1175"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1175"/>
    <dbReference type="eggNOG" id="COG0583">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039613_37_2_4"/>
    <dbReference type="OMA" id="PGSWESW"/>
    <dbReference type="OrthoDB" id="846433at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005119">
        <property type="entry name" value="LysR_subst-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000847">
        <property type="entry name" value="Tscrpt_reg_HTH_LysR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00126">
        <property type="entry name" value="HTH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03466">
        <property type="entry name" value="LysR_substrate"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00039">
        <property type="entry name" value="HTHLYSR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50931">
        <property type="entry name" value="HTH_LYSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH lysR-type" evidence="2">
        <location>
            <begin position="7"/>
            <end position="62"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF08061.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="299" mass="32461" checksum="E7F2BA34D385F952" modified="2006-05-30" version="1">MRTLPPMSTLRAFEATARTGSVTRAAEELFRTHGAVSRQLRQLQEHAGVELFEREGTGLRLNEHGQALYEVVRTALDQLEQGYGRLLDQARGPTLHVACSATFAMRWLVPELVDFYRLRPDIRIRLSMTSAREIRTEGADVVIAWDLSSYPAAERQRAIPLAPVAFGPVCAPAYAPEVATGARITHDFTSSAWDQWEALTGHHIAPGAGLTFPHTHLCIQAALSGLGVALIEQRLIQAELAAGTLVAPFGFTAFGGGLMAVPATRDVPADADVFIAWLQRRLLPPVDSRAPRQAGQTGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-12-02" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LPH6</accession>
    <name>Q1LPH6_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative metal-dependent phosphoesterase (PHP family)</fullName>
            <ecNumber evidence="2">3.1.-.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_1064</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EC" id="3.1.-.-" evidence="2"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07950.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1064"/>
    <dbReference type="EnsemblBacteria" id="ABF07950">
        <property type="protein sequence ID" value="ABF07950"/>
        <property type="gene ID" value="Rmet_1064"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1064"/>
    <dbReference type="eggNOG" id="COG0613">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_067347_0_0_4"/>
    <dbReference type="OMA" id="CTWGGAT"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004013">
        <property type="entry name" value="PHP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003141">
        <property type="entry name" value="Pol/His_phosphatase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016195">
        <property type="entry name" value="Pol/histidinol_Pase-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02811">
        <property type="entry name" value="PHP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00481">
        <property type="entry name" value="POLIIIAc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89550">
        <property type="entry name" value="SSF89550"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="POLIIIAc" evidence="1">
        <location>
            <begin position="50"/>
            <end position="115"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF07950.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="322" mass="34474" checksum="A8A7A5CA5BEA3766" modified="2006-05-30" version="1">MADEEGIGFVTVPSYLAVVGNPGTPPLLAGLKSPAGSRMVAMQNAHAINADLHCHSTVSDGLLSPSDVAARAHAGGVAFWSLTDHDEVGGQIEARRAAEALGMRYVPGVEISVTWAGQTVHIVGLQIDPFHPELIDGLAKTRDGRARRAQDIGDALAKVGISGAYDGALRYVGNPDLISRTHFARWLVDEGRCATIGDVFAEYLSEGRPGFVPHRWATLAEAVGWIRTAGGIPVMAHPGRYNYTDTQHDALFDEFTQLGGRAVEVVTGSHTPDQFRRYANVARHYGLLASRGSDFHGPGEGRVELGALPPLPADVTPVWYDW</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="115" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LPM1</accession>
    <name>Q1LPM1_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="13 14 15">UvrABC system protein B</fullName>
            <shortName evidence="14">Protein UvrB</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Excinuclease ABC subunit B</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14 20" type="primary">uvrB</name>
        <name evidence="20" type="ordered locus">Rmet_1019</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11 14">The UvrABC repair system catalyzes the recognition and processing of DNA lesions. A damage recognition complex composed of 2 UvrA and 2 UvrB subunits scans DNA for abnormalities. Upon binding of the UvrA(2)B(2) complex to a putative damaged site, the DNA wraps around one UvrB monomer. DNA wrap is dependent on ATP binding by UvrB and probably causes local melting of the DNA helix, facilitating insertion of UvrB beta-hairpin between the DNA strands. Then UvrB probes one DNA strand for the presence of a lesion. If a lesion is found the UvrA subunits dissociate and the UvrB-DNA preincision complex is formed. This complex is subsequently bound by UvrC and the second UvrB is released. If no lesion is found, the DNA wraps around the other UvrB subunit that will check the other stand for damage.</text>
    </comment>
    <comment type="subunit">
        <text evidence="12 14 15">Forms a heterotetramer with UvrA during the search for lesions. Interacts with UvrC in an incision complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 14 15">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="14">The beta-hairpin motif is involved in DNA binding.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 14 15">Belongs to the UvrB family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07905.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515816.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1019"/>
    <dbReference type="EnsemblBacteria" id="ABF07905">
        <property type="protein sequence ID" value="ABF07905"/>
        <property type="gene ID" value="Rmet_1019"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1019"/>
    <dbReference type="eggNOG" id="COG0556">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009621_2_1_4"/>
    <dbReference type="OMA" id="EYVDRMV"/>
    <dbReference type="OrthoDB" id="95696at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009380">
        <property type="term" value="C:excinuclease repair complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009381">
        <property type="term" value="F:excinuclease ABC activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006289">
        <property type="term" value="P:nucleotide-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009432">
        <property type="term" value="P:SOS response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00204">
        <property type="entry name" value="UvrB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006935">
        <property type="entry name" value="Helicase/UvrB_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001943">
        <property type="entry name" value="UVR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036876">
        <property type="entry name" value="UVR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004807">
        <property type="entry name" value="UvrB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041471">
        <property type="entry name" value="UvrB_inter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024759">
        <property type="entry name" value="UvrB_YAD/RRR_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24029">
        <property type="entry name" value="PTHR24029"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04851">
        <property type="entry name" value="ResIII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02151">
        <property type="entry name" value="UVR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12344">
        <property type="entry name" value="UvrB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17757">
        <property type="entry name" value="UvrB_inter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46600">
        <property type="entry name" value="SSF46600"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00631">
        <property type="entry name" value="uvrb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50151">
        <property type="entry name" value="UVR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="16" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3 14" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 14 15" id="KW-0227">DNA damage</keyword>
    <keyword evidence="6 14 15" id="KW-0228">DNA excision</keyword>
    <keyword evidence="9 14 15" id="KW-0234">DNA repair</keyword>
    <keyword evidence="8 14 15" id="KW-0267">Excision nuclease</keyword>
    <keyword evidence="4 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10 14 15" id="KW-0742">SOS response</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="18">
        <location>
            <begin position="45"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="19">
        <location>
            <begin position="449"/>
            <end position="615"/>
        </location>
    </feature>
    <feature type="domain" description="UVR" evidence="17">
        <location>
            <begin position="646"/>
            <end position="681"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="16">
        <location>
            <begin position="642"/>
            <end position="669"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Beta-hairpin" evidence="14">
        <location>
            <begin position="111"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <begin position="58"/>
            <end position="65"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00025647"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00026033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00029504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU003587"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="ABF07905.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="697" mass="79320" checksum="E3F1911EC3C43D4A" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="96" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LPV9</accession>
    <name>Q1LPV9_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">NADH dehydrogenase chain E</fullName>
            <ecNumber evidence="7">1.6.99.5</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">nuoE</name>
        <name evidence="7" type="ordered locus">Rmet_0931</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>2Fe-2S cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49601"/>
        </cofactor>
        <text evidence="6">Binds 1 [2Fe-2S] cluster.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the complex I 24 kDa subunit family.</text>
    </comment>
    <dbReference type="EC" id="1.6.99.5" evidence="7"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07817.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0931"/>
    <dbReference type="EnsemblBacteria" id="ABF07817">
        <property type="protein sequence ID" value="ABF07817"/>
        <property type="gene ID" value="Rmet_0931"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0931"/>
    <dbReference type="eggNOG" id="COG1905">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_054362_2_0_4"/>
    <dbReference type="OMA" id="MYDTKPV"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03064">
        <property type="entry name" value="TRX_Fd_NuoE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.1590">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002023">
        <property type="entry name" value="NuoE-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042128">
        <property type="entry name" value="NuoE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041921">
        <property type="entry name" value="NuoE_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000216">
        <property type="entry name" value="NADH_DH_24kDa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="SSF52833"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01958">
        <property type="entry name" value="nuoE_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01099">
        <property type="entry name" value="COMPLEX1_24K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 6" id="KW-0001">2Fe-2S</keyword>
    <keyword evidence="4 6" id="KW-0408">Iron</keyword>
    <keyword evidence="5 6" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="3 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="6">
        <location>
            <position position="84"/>
        </location>
        <ligand>
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="89"/>
        </location>
        <ligand>
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="125"/>
        </location>
        <ligand>
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="129"/>
        </location>
        <ligand>
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR000216-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABF07817.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="168" mass="18260" checksum="833F5BE426B9DB2D" modified="2006-05-30" version="1">MTMLSAEALKEIDRAIAKYPADQKQSAVMAALAVAQGEVGWVSPEVMQFVASYLEMPPVWVEEVATFYNMYDTKPVGKHKLAVCTNLPCALSGGERAGEYLKRKLGIDYNETTADGCFTLKEGECMGACGDAPVMIVNNTRMCSFMSEQKIDALVEELKSEAAAKGDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LQ01</accession>
    <name>Q1LQ01_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 5">Guanine deaminase</fullName>
            <shortName evidence="5">Guanase</shortName>
            <ecNumber evidence="3 5">3.5.4.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Guanine aminohydrolase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">guaD</name>
        <name evidence="7" type="ordered locus">Rmet_0889</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Catalyzes the hydrolytic deamination of guanine, producing xanthine and ammonia.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>guanine + H(+) + H2O = NH4(+) + xanthine</text>
            <dbReference type="Rhea" id="RHEA:14665"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16235"/>
            <dbReference type="ChEBI" id="CHEBI:17712"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="EC" id="3.5.4.3"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="5">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1 5">Purine metabolism; guanine degradation; xanthine from guanine: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the metallo-dependent hydrolases superfamily. ATZ/TRZ family.</text>
    </comment>
    <dbReference type="EC" id="3.5.4.3" evidence="3 5"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07775.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0889"/>
    <dbReference type="EnsemblBacteria" id="ABF07775">
        <property type="protein sequence ID" value="ABF07775"/>
        <property type="gene ID" value="Rmet_0889"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0889"/>
    <dbReference type="eggNOG" id="COG0402">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012358_0_2_4"/>
    <dbReference type="OMA" id="ALIGKVC"/>
    <dbReference type="UniPathway" id="UPA00603">
        <property type="reaction ID" value="UER00660"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008892">
        <property type="term" value="F:guanine deaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006147">
        <property type="term" value="P:guanine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01303">
        <property type="entry name" value="GDEase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006680">
        <property type="entry name" value="Amidohydro-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014311">
        <property type="entry name" value="Guanine_deaminase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011059">
        <property type="entry name" value="Metal-dep_hydrolase_composite"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11271:SF6">
        <property type="entry name" value="PTHR11271:SF6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01979">
        <property type="entry name" value="Amidohydro_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51338">
        <property type="entry name" value="SSF51338"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="SSF51556"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02967">
        <property type="entry name" value="guan_deamin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Amidohydro-rel" evidence="6">
        <location>
            <begin position="80"/>
            <end position="439"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU366009"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABF07775.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="446" mass="49566" checksum="EA235DB634A101FC" modified="2006-05-30" version="1">MKMTTTSPASGADNAVTRAIRGRILHFLRDPQFHDDAYQYWEDGLMIVKAGRIAAVGNYADLSRDIPQGAEVVDHRGKLIVPGFIDTHIHYPQTDIIASPSPGLLHWLDTYTFPEERRFSDPAYARAVADFFTDELLRNGTTTAMVWSTVHAASADALFAASQQRHMRMITGKVLMDRNCPDFLRDTAESGARESADLISKWHNVDRLAYAITPRFAPTSTEAQLEACGELAKAHSDVFIQTHVAENRDEVKWVAELFPDARSYLDVYDRYKLLRPGAFYGHAIYLDEDDRRRLADSGAAVAHCPTSNLFLGSGFYDFHQSDANRLNVTLATDVGGGTSFSMFRTMNAAHKVARMGGYHLTALRMFYLATRAAAEALGWQDRIGSFEVGCEADFVVLDPDATPLISRRSSRSETLEEQLFAFAMLGDDRVIENVYIMGEATSPVAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LQ24</accession>
    <name>Q1LQ24_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Aspartyl/Asparaginyl beta-hydroxylase</fullName>
            <ecNumber evidence="3">1.14.11.16</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">aspH</name>
        <name evidence="3" type="ordered locus">Rmet_0866</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EC" id="1.14.11.16" evidence="3"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07752.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515693.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0866"/>
    <dbReference type="EnsemblBacteria" id="ABF07752">
        <property type="protein sequence ID" value="ABF07752"/>
        <property type="gene ID" value="Rmet_0866"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0866"/>
    <dbReference type="eggNOG" id="COG3555">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_927100_0_0_4"/>
    <dbReference type="OMA" id="YAGSYRY"/>
    <dbReference type="OrthoDB" id="1564693at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0062101">
        <property type="term" value="F:peptidyl-aspartic acid 3-dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018193">
        <property type="term" value="P:peptidyl-amino acid modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007803">
        <property type="entry name" value="Asp/Arg/Pro-Hydrxlase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027443">
        <property type="entry name" value="IPNS-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05118">
        <property type="entry name" value="Asp_Arg_Hydrox"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="281"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="domain" description="Asp_Arg_Hydrox" evidence="2">
        <location>
            <begin position="70"/>
            <end position="223"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF05118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF07752.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="300" mass="34804" checksum="D9ECD7BDA36DE820" modified="2006-05-30" version="1">MIKWIIVAAYLGAILYVHFRGKVRLPFMRQLLDHSALVAPFNCFMYIFSGVPRTPYIPVERFPDLEKIRSAWPQIRDEGLALIAMRKIKAADKNDDAGFNSFFKYGWKRFYLKWYEAQHPSAEVLCPNTVALLRDMPSVKAAMFAELPPGGKLNPHRDPFAGSLRYHLGLATPNDDRCFIEVDGERHSWRDGQGVVFDETYLHWAENKSETDRLILFCDIERPMRYRWAAAVNHWLGRKVMTAASSPNDENDQTGMINKLFRFVWLGGQYRRRFKAWNKNLYYVTKFGLIIGGVALIVFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LQA1</accession>
    <name>Q1LQA1_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Extra-cytoplasmic Solute Receptor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">bug</name>
        <name evidence="4" type="ordered locus">Rmet_0789</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Periplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the UPF0065 (bug) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07675.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515627.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0789"/>
    <dbReference type="EnsemblBacteria" id="ABF07675">
        <property type="protein sequence ID" value="ABF07675"/>
        <property type="gene ID" value="Rmet_0789"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0789"/>
    <dbReference type="eggNOG" id="COG3181">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045683_0_0_4"/>
    <dbReference type="OrthoDB" id="447734at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042597">
        <property type="term" value="C:periplasmic space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005064">
        <property type="entry name" value="BUG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042100">
        <property type="entry name" value="Bug_dom1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42928">
        <property type="entry name" value="PTHR42928"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03401">
        <property type="entry name" value="TctC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017082">
        <property type="entry name" value="YflP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0675">Receptor</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193336" evidence="3">
        <location>
            <begin position="30"/>
            <end position="329"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF07675.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="329" mass="34445" checksum="7BB18BA5FF5E4F6E" modified="2010-03-23" version="2">MPSYRFTARRRAILSATLLAFAVPSAAFAADSFPSKPIRFIVPYAAGGTTDLVARTVGQRVAEKLGQPVVIENRPGAGGNIGMDAVAKAPADGYTIGFGAISTNALNPHIYKHVPFDPRRDFTAISLLGTSTIVLEVSESLPVKTVPELIAYAKAHPGLTYATAGTGTSMHLAGAMFSQVTQTNLTHVPYKGSSPAINDMLGGHIQVMFDNLPASLPHIQAGKLRALAVAGKTRSPSLPNVPTLAEAGLPGYAVEPWFGVYGPANLPAPVVQALNTAFVEALARPDVRDKLAQAGFNPKGSSAAELQTLTQTEYERFGKVAKSAGITVD</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2007-01-09" modified="2022-08-03" version="99" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LQD3</accession>
    <name>MSBA_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">ATP-dependent lipid A-core flippase</fullName>
            <ecNumber evidence="1">7.5.2.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Lipid A export ATP-binding/permease protein MsbA</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">msbA</name>
        <name type="ordered locus">Rmet_0757</name>
    </gene>
    <organism>
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in lipopolysaccharide (LPS) biosynthesis. Translocates lipid A-core from the inner to the outer leaflet of the inner membrane. Transmembrane domains (TMD) form a pore in the inner membrane and the ATP-binding domain (NBD) is responsible for energy generation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + H2O + lipid A-core oligosaccharideSide 1 = ADP + phosphate + lipid A-core oligosaccharideSide 2.</text>
            <dbReference type="EC" id="7.5.2.6"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="1">In MsbA the ATP-binding domain (NBD) and the transmembrane domain (TMD) are fused.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the ABC transporter superfamily. Lipid exporter (TC 3.A.1.106) family.</text>
    </comment>
    <dbReference type="EC" id="7.5.2.6" evidence="1"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07643.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q1LQD3"/>
    <dbReference type="SMR" id="Q1LQD3"/>
    <dbReference type="STRING" id="266264.Rmet_0757"/>
    <dbReference type="EnsemblBacteria" id="ABF07643">
        <property type="protein sequence ID" value="ABF07643"/>
        <property type="gene ID" value="Rmet_0757"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0757"/>
    <dbReference type="eggNOG" id="COG1132">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_84_3_4"/>
    <dbReference type="OMA" id="WGTYLVK"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034040">
        <property type="term" value="F:ATPase-coupled lipid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1560.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011527">
        <property type="entry name" value="ABC1_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036640">
        <property type="entry name" value="ABC1_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011917">
        <property type="entry name" value="ABC_transpr_lipidA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039421">
        <property type="entry name" value="Type_1_exporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24221">
        <property type="entry name" value="PTHR24221"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00664">
        <property type="entry name" value="ABC_membrane"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90123">
        <property type="entry name" value="SSF90123"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02203">
        <property type="entry name" value="MsbA_lipidA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50929">
        <property type="entry name" value="ABC_TM1F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51239">
        <property type="entry name" value="MSBA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0997">Cell inner membrane</keyword>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0445">Lipid transport</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-1278">Translocase</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <keyword id="KW-0813">Transport</keyword>
    <feature type="chain" id="PRO_0000271647" description="ATP-dependent lipid A-core flippase">
        <location>
            <begin position="1"/>
            <end position="594"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="35"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="64"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="135"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="161"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="262"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="289"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="1">
        <location>
            <begin position="36"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="1">
        <location>
            <begin position="350"/>
            <end position="588"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="384"/>
            <end position="391"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01703"/>
        </source>
    </evidence>
    <sequence length="594" mass="64433" checksum="2EC4BAB37B8D47BB" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="102" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LQF0</accession>
    <name>Q1LQF0_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">D-amino acid dehydrogenase</fullName>
            <ecNumber evidence="4">1.4.99.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4 6" type="primary">dadA</name>
        <name evidence="6" type="ordered locus">Rmet_0740</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Oxidative deamination of D-amino acids.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 4">
            <text>A + a D-alpha-amino acid + H2O = a 2-oxocarboxylate + AH2 + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:18125"/>
            <dbReference type="ChEBI" id="CHEBI:13193"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:17499"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:35179"/>
            <dbReference type="ChEBI" id="CHEBI:59871"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 4">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3 4">Belongs to the DadA oxidoreductase family.</text>
    </comment>
    <dbReference type="EC" id="1.4.99.-" evidence="4"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07626.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008643686.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0740"/>
    <dbReference type="EnsemblBacteria" id="ABF07626">
        <property type="protein sequence ID" value="ABF07626"/>
        <property type="gene ID" value="Rmet_0740"/>
    </dbReference>
    <dbReference type="GeneID" id="60825227"/>
    <dbReference type="KEGG" id="rme:Rmet_0740"/>
    <dbReference type="eggNOG" id="COG0665">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007884_9_2_4"/>
    <dbReference type="OMA" id="NDLYPRG"/>
    <dbReference type="OrthoDB" id="573710at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008718">
        <property type="term" value="F:D-amino-acid dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019478">
        <property type="term" value="P:D-amino acid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01202">
        <property type="entry name" value="DadA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023080">
        <property type="entry name" value="DadA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006076">
        <property type="entry name" value="FAD-dep_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01266">
        <property type="entry name" value="DAO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="SSF51905"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0274">FAD</keyword>
    <keyword evidence="4" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="4 6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DAO" evidence="5">
        <location>
            <begin position="2"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="3"/>
            <end position="17"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009410"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABF07626.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="434" mass="46969" checksum="36C901D135166266" modified="2006-05-30" version="1">MRVLILGSGVIGVTSAWYLARAGHEVIVVDREGGPALGTSFANAGQISPGYASPWAAPGVPLKAIKWMFQEHAPLTIKPDGTLFQLQWMWQMLQNCTADRYAVNKERMVRLAEYSRDCIRALRADTGISYEGRQQGTLQVFRTDAQLEAASRDIAVLEEAGVPYELLSREELAASEPALAAVGHKLAGGLRLPNDETGDCQLFTERLAAMAESIGVQFIYNRSIDGLLTQGDAVTGAIVEGQPMMADAVVVALGSWSTPFVKHVLPGLSNLPVYPLKGFSITVPLTDASKSPVSTVLDETYKVAITRFDDRIRVGGMAQIVGYDRSLDPNKRRTLEHVVNDLFPGAGDVSRATFWTGLRPMTPDSTPVVGPAGIRGLWLNTGHGTLGWTMACGSGQLLSDLVSGTRPAIRADDLSVDRYRKQPRTHHLPRPLPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LQK0</accession>
    <name>Q1LQK0_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Putative amino-acid-binding periplasmic abc transporter protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Rmet_0690</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the bacterial solute-binding protein 3 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07576.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008643603.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0690"/>
    <dbReference type="EnsemblBacteria" id="ABF07576">
        <property type="protein sequence ID" value="ABF07576"/>
        <property type="gene ID" value="Rmet_0690"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0690"/>
    <dbReference type="eggNOG" id="COG0834">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019602_18_4_4"/>
    <dbReference type="OMA" id="SADWPPY"/>
    <dbReference type="OrthoDB" id="685252at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018313">
        <property type="entry name" value="SBP_3_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001638">
        <property type="entry name" value="Solute-binding_3/MltF_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00497">
        <property type="entry name" value="SBP_bac_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00062">
        <property type="entry name" value="PBPb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01039">
        <property type="entry name" value="SBP_BACTERIAL_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193432" evidence="4">
        <location>
            <begin position="31"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="domain" description="PBPb" evidence="5">
        <location>
            <begin position="41"/>
            <end position="264"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010333"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003744"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00062"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABF07576.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="282" mass="30411" checksum="E356B61704912869" modified="2006-05-30" version="1">MQADFRRKLRQIAGVATLSALALSAAPASADQIADIKAKGQLVCGVLGTDEPFSFVDPATREIVGYDVDMCNAVAKSLGVKPVLKQISVAARLPELQQGHVDLLAASLTHNKERESQIDFSLSTFITGQKAMVRKESGITTLGQLDGKKILTVKGSTMESNIAAKIKNATVVSFDNSPQALLALEQGKGVAYVNDETSLVSNLVKLGPAAKDYTLVPQYLSSEHLALGIRKGETGFRNQVNQVLSKMETDGDAQKLYDKWFGPNTKMKFPPRAFKISTDKID</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="98" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LQK5</accession>
    <name>Q1LQK5_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="12">DNA segregation ATPase ftsk/spoIIIE protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">ftsK</name>
        <name evidence="12" type="ordered locus">Rmet_0685</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Essential cell division protein that coordinates cell division and chromosome segregation. The N-terminus is involved in assembly of the cell-division machinery. The C-terminus functions as a DNA motor that moves dsDNA in an ATP-dependent manner towards the dif recombination site, which is located within the replication terminus region. Translocation stops specifically at Xer-dif sites, where FtsK interacts with the Xer recombinase, allowing activation of chromosome unlinking by recombination. FtsK orienting polar sequences (KOPS) guide the direction of DNA translocation. FtsK can remove proteins from DNA as it translocates, but translocation stops specifically at XerCD-dif site, thereby preventing removal of XerC and XerD from dif.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the FtsK/SpoIIIE/SftA family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07571.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515538.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0685"/>
    <dbReference type="EnsemblBacteria" id="ABF07571">
        <property type="protein sequence ID" value="ABF07571"/>
        <property type="gene ID" value="Rmet_0685"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0685"/>
    <dbReference type="eggNOG" id="COG1674">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001981_9_7_4"/>
    <dbReference type="OMA" id="YKAEARD"/>
    <dbReference type="OrthoDB" id="349533at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025199">
        <property type="entry name" value="FtsK_4TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041027">
        <property type="entry name" value="FtsK_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002543">
        <property type="entry name" value="FtsK_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018541">
        <property type="entry name" value="Ftsk_gamma"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13491">
        <property type="entry name" value="FtsK_4TM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17854">
        <property type="entry name" value="FtsK_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09397">
        <property type="entry name" value="FtsK_gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01580">
        <property type="entry name" value="FtsK_SpoIIIE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00843">
        <property type="entry name" value="Ftsk_gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50901">
        <property type="entry name" value="FTSK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="28"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="68"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="118"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="160"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="domain" description="FtsK" evidence="11">
        <location>
            <begin position="411"/>
            <end position="620"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="428"/>
            <end position="435"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006474"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00024784"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50901"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ABF07571.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="775" mass="84238" checksum="C86EFDF2E60AF5A5" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="65" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LQU4</accession>
    <name>Q1LQU4_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative transmembrane transcriptional regulator (Anti-sigma factor)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_0596</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07482.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515455.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0596"/>
    <dbReference type="EnsemblBacteria" id="ABF07482">
        <property type="protein sequence ID" value="ABF07482"/>
        <property type="gene ID" value="Rmet_0596"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0596"/>
    <dbReference type="eggNOG" id="COG5662">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_058379_0_0_4"/>
    <dbReference type="OMA" id="KPWFNGK"/>
    <dbReference type="OrthoDB" id="1494577at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.1320">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041916">
        <property type="entry name" value="Anti_sigma_zinc_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027383">
        <property type="entry name" value="Znf_put"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13490">
        <property type="entry name" value="zf-HC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <feature type="domain" description="zf-HC2" evidence="1">
        <location>
            <begin position="3"/>
            <end position="36"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF07482.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="266" mass="28745" checksum="126C9FD63A85279C" modified="2006-05-30" version="1">MDCNDARNLLQACADGEVGAADSVRLERHLEDCESCVDSLRNLQALRAATRHGAAYHRAPADLRARILASLPAPAEPGFVDSGAAEPAGWRSWFTWSPAVNAALATITAAAVGLSLFQFNQQPSEGDLTANAIVASHVRGLISNRAIDVVSSDQHTVKPWFNGRIDYAPDVRDLGAAGFPLVGGRLDYVDGQRVAVIVYRRNQHPIDVFVIPGNNSGALGERERQGYQIETWSAHGMRYWAVTDASAGDLRAFVQAWRNAEGGVER</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LR73</accession>
    <name>Q1LR73_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 4">Regulatory protein RecX</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4 5" type="primary">recX</name>
        <name evidence="5" type="ordered locus">Rmet_0467</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Modulates RecA activity.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 4">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 4">Belongs to the RecX family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07353.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0467"/>
    <dbReference type="EnsemblBacteria" id="ABF07353">
        <property type="protein sequence ID" value="ABF07353"/>
        <property type="gene ID" value="Rmet_0467"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0467"/>
    <dbReference type="eggNOG" id="COG2137">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_066607_3_1_4"/>
    <dbReference type="OMA" id="RCKMQEK"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006282">
        <property type="term" value="P:regulation of DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01114">
        <property type="entry name" value="RecX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003783">
        <property type="entry name" value="Regulatory_RecX"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33602">
        <property type="entry name" value="PTHR33602"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02631">
        <property type="entry name" value="RecX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00018111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABF07353.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="182" mass="20906" checksum="AAF1C43B6EED7993" modified="2006-05-30" version="1">MRRLFCFHPGFPYRAAPTAGFLVISRMPRPPLSLKARAVGYLSRREHSRAELARKLAPHAESAEEVEALLDVLERDNWLSNERFVDSLVHRRASRYGAARVLQEAKTHQLGNDQLRDLQDRLRDSELDRAREVWRKRFGVPPDSPEARAKQIRFMMARGFSRSVVGKIIRGAEDEGMDDLGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="112" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LR91</accession>
    <name>Q1LR91_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Apolipoprotein N-acyltransferase</fullName>
            <shortName evidence="9">ALP N-acyltransferase</shortName>
            <ecNumber evidence="9">2.3.1.269</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9 11" type="primary">lnt</name>
        <name evidence="11" type="ordered locus">Rmet_0449</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Catalyzes the phospholipid dependent N-acylation of the N-terminal cysteine of apolipoprotein, the last step in lipoprotein maturation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>a glycerophospholipid + N-terminal S-1,2-diacyl-sn-glyceryl-L-cysteinyl-[lipoprotein] = a 2-acyl-sn-glycero-3-phospholipid + H(+) + N-acyl-S-1,2-diacyl-sn-glyceryl-L-cysteinyl-[lipoprotein]</text>
            <dbReference type="Rhea" id="RHEA:48228"/>
            <dbReference type="Rhea" id="RHEA-COMP:14681"/>
            <dbReference type="Rhea" id="RHEA-COMP:14684"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:136912"/>
            <dbReference type="ChEBI" id="CHEBI:140656"/>
            <dbReference type="ChEBI" id="CHEBI:140657"/>
            <dbReference type="ChEBI" id="CHEBI:140660"/>
            <dbReference type="EC" id="2.3.1.269"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="9">Protein modification; lipoprotein biosynthesis (N-acyl transfer).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cell inner membrane</location>
            <topology evidence="9">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the CN hydrolase family. Apolipoprotein N-acyltransferase subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.269" evidence="9"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07335.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0449"/>
    <dbReference type="EnsemblBacteria" id="ABF07335">
        <property type="protein sequence ID" value="ABF07335"/>
        <property type="gene ID" value="Rmet_0449"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0449"/>
    <dbReference type="eggNOG" id="COG0815">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019563_3_0_4"/>
    <dbReference type="OMA" id="IPQSMKW"/>
    <dbReference type="UniPathway" id="UPA00666"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016410">
        <property type="term" value="F:N-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042158">
        <property type="term" value="P:lipoprotein biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd07571">
        <property type="entry name" value="ALP_N-acyl_transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.110.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01148">
        <property type="entry name" value="Lnt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004563">
        <property type="entry name" value="Apolipo_AcylTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003010">
        <property type="entry name" value="C-N_Hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036526">
        <property type="entry name" value="C-N_Hydrolase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045378">
        <property type="entry name" value="LNT_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00795">
        <property type="entry name" value="CN_hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20154">
        <property type="entry name" value="LNT_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56317">
        <property type="entry name" value="SSF56317"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00546">
        <property type="entry name" value="lnt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50263">
        <property type="entry name" value="CN_HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 9 11" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="9" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="3 9" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9 11" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="34"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="58"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="88"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="127"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="162"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="200"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="483"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="domain" description="CN hydrolase" evidence="10">
        <location>
            <begin position="235"/>
            <end position="476"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01148"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ABF07335.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="510" mass="55053" checksum="ED369D6D4841E3F2" modified="2006-05-30" version="1">MQVTSRSGTVFRLLIAAVLGVAHTQAFAPHNWWWLQILSLAGLVALIAGAPRPRAAAATAYAFGLGWFLSGIWWLFISMHVYGEMPSWMAGLAVVLFAGYLSIWPALAGAAWHWLTQRGRNGVLPALAFGATWALSEWLRGVVFTGFPWLGSGYPHTDGPLAGFAPLIGVYGISALAGFIAALLVAVVGAIGRQRKARSAVLAGGLAVGVLACGAALAPIQYTSPIGQPIHVRLLQGNVAQDIKFEPVGIERSLELYRDLITEAPADLVVTPETAFPVILQELPVEIARAVRTFMEKTGTTIIFGAAGADSPVDFTNSVFAIGPEQDQLYRYNKHHLVPFGEFIPFGFRWFVDMMKMPLGDFRRGTLDQPAVPVRGIHVAPNICYEDLFGEEIAATLRQQATPANMLANVTNLAWFGDTIALDQHLQISRMRALEMRRPMLRATNTGMTAVVAPDGTVQAKLPTFQVGTLTATVQGMQGLTPYIRWGNVPVLIGSLLVLLVAARARRARG</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="72" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LRF9</accession>
    <name>Q1LRF9_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative endonuclease/exonuclease/phosphatase protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_0381</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07267.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515259.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0381"/>
    <dbReference type="EnsemblBacteria" id="ABF07267">
        <property type="protein sequence ID" value="ABF07267"/>
        <property type="gene ID" value="Rmet_0381"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0381"/>
    <dbReference type="eggNOG" id="COG3568">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_060500_3_2_4"/>
    <dbReference type="OMA" id="DHGHHGN"/>
    <dbReference type="OrthoDB" id="1387978at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004527">
        <property type="term" value="F:exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005135">
        <property type="entry name" value="Endo/exonuclease/phosphatase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03372">
        <property type="entry name" value="Exo_endo_phos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="SSF56219"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="2" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0540">Nuclease</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Endo/exonuclease/phosphatase" evidence="1">
        <location>
            <begin position="6"/>
            <end position="235"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03372"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF07267.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="251" mass="28426" checksum="58762F8D34E82961" modified="2006-05-30" version="1">MKLRVVTYNIHKGVKGFTGKPRIQSVRTGLQSMDADIVFLQEVQDRNDRLIAAELFDPDHTQLNYLATDAYPHSVYGRNAVYDHGHHGNAILSRHPILMSENLDISDHRFEQRGLLHAVADINGVEAHLICVHFGLFAGSRVRQTTALVERVRSVVPADAPLVIAGDFNDWNHKLDSKICNTLNAVESANAKNGRIHTFPSHMPWWQLDRIYARGFEFERTQALTGREWAQRSDHVPLMAELARRVAIPEV</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-05-20" modified="2022-08-03" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LRQ0</accession>
    <name>ISPE_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">4-diphosphocytidyl-2-C-methyl-D-erythritol kinase</fullName>
            <shortName evidence="1">CMK</shortName>
            <ecNumber evidence="1">2.7.1.148</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol kinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">ispE</name>
        <name type="ordered locus">Rmet_0290</name>
    </gene>
    <organism>
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the phosphorylation of the position 2 hydroxy group of 4-diphosphocytidyl-2C-methyl-D-erythritol.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>4-CDP-2-C-methyl-D-erythritol + ATP = 4-CDP-2-C-methyl-D-erythritol 2-phosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:18437"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57823"/>
            <dbReference type="ChEBI" id="CHEBI:57919"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.148"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 3/6.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the GHMP kinase family. IspE subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.148" evidence="1"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07176.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515178.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q1LRQ0"/>
    <dbReference type="SMR" id="Q1LRQ0"/>
    <dbReference type="STRING" id="266264.Rmet_0290"/>
    <dbReference type="EnsemblBacteria" id="ABF07176">
        <property type="protein sequence ID" value="ABF07176"/>
        <property type="gene ID" value="Rmet_0290"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0290"/>
    <dbReference type="eggNOG" id="COG1947">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_053057_3_0_4"/>
    <dbReference type="OMA" id="RWPSPAK"/>
    <dbReference type="OrthoDB" id="1938933at2"/>
    <dbReference type="UniPathway" id="UPA00056">
        <property type="reaction ID" value="UER00094"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050515">
        <property type="term" value="F:4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019288">
        <property type="term" value="P:isopentenyl diphosphate biosynthetic process, methylerythritol 4-phosphate pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016114">
        <property type="term" value="P:terpenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.890">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00061">
        <property type="entry name" value="IspE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013750">
        <property type="entry name" value="GHMP_kinase_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036554">
        <property type="entry name" value="GHMP_kinase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006204">
        <property type="entry name" value="GHMP_kinase_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004424">
        <property type="entry name" value="IspE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43527:SF2">
        <property type="entry name" value="PTHR43527:SF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08544">
        <property type="entry name" value="GHMP_kinases_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00288">
        <property type="entry name" value="GHMP_kinases_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF010376">
        <property type="entry name" value="IspE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="SSF54211"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55060">
        <property type="entry name" value="SSF55060"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00154">
        <property type="entry name" value="ispE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0414">Isoprene biosynthesis</keyword>
    <keyword id="KW-0418">Kinase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000335744" description="4-diphosphocytidyl-2-C-methyl-D-erythritol kinase">
        <location>
            <begin position="1"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="20"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="145"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="103"/>
            <end position="113"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00061"/>
        </source>
    </evidence>
    <sequence length="292" mass="31781" checksum="B700AEA8E1E73486" modified="2006-05-30" version="1">MTVGTALPPPELRNCPAPAKLNLFLHVTGRRPDGYHTLQTVFQLIDWSDTLHFHRRDDGVITRVTDIPGVPADTDLVVRAARAMQAASGTRFGVDIAIDKILPMGGGIGGGSSDAATTLLALNRLWGVNLPREELMRIGLSLGADVPVFVFGQNAFAEGVGEELTPVALPDSWFVVIHPRQHVPTAAIFSDERLTRNSPISIVADFAACTNKFAFGRNDLETIATAKFGEVARALEWLKNYSPHARMTGSGACVFARFEDEQTAQRVMERLPSEWDGRCVKSLSHHPLATFA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="108" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LSA2</accession>
    <name>Q1LSA2_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Methionine synthase</fullName>
            <ecNumber evidence="5">2.1.1.13</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">5-methyltetrahydrofolate--homocysteine methyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="primary">metH</name>
        <name evidence="13" type="ordered locus">Rmet_0088</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Catalyzes the transfer of a methyl group from methyl-cobalamin to homocysteine, yielding enzyme-bound cob(I)alamin and methionine. Subsequently, remethylates the cofactor using methyltetrahydrofolate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>(6S)-5-methyl-5,6,7,8-tetrahydrofolate + L-homocysteine = (6S)-5,6,7,8-tetrahydrofolate + L-methionine</text>
            <dbReference type="Rhea" id="RHEA:11172"/>
            <dbReference type="ChEBI" id="CHEBI:18608"/>
            <dbReference type="ChEBI" id="CHEBI:57453"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:58199"/>
            <dbReference type="EC" id="2.1.1.13"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="5 7">
            <name>methylcob(III)alamin</name>
            <dbReference type="ChEBI" id="CHEBI:28115"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="5">Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-methionine from L-homocysteine (MetH route): step 1/1.</text>
    </comment>
    <comment type="domain">
        <text evidence="5">Modular enzyme with four functionally distinct domains. The isolated Hcy-binding domain catalyzes methyl transfer from free methylcobalamin to homocysteine. The Hcy-binding domain in association with the pterin-binding domain catalyzes the methylation of cob(I)alamin by methyltetrahydrofolate and the methylation of homocysteine. The B12-binding domain binds the cofactor. The AdoMet activation domain binds S-adenosyl-L-methionine. Under aerobic conditions cob(I)alamin can be converted to inactive cob(II)alamin. Reductive methylation by S-adenosyl-L-methionine and flavodoxin regenerates methylcobalamin.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the vitamin-B12 dependent methionine synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.13" evidence="5"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF06974.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0088"/>
    <dbReference type="EnsemblBacteria" id="ABF06974">
        <property type="protein sequence ID" value="ABF06974"/>
        <property type="gene ID" value="Rmet_0088"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0088"/>
    <dbReference type="eggNOG" id="COG1410">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004914_2_0_4"/>
    <dbReference type="OMA" id="ADCIAMS"/>
    <dbReference type="UniPathway" id="UPA00051">
        <property type="reaction ID" value="UER00081"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031419">
        <property type="term" value="F:cobalamin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008705">
        <property type="term" value="F:methionine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042558">
        <property type="term" value="P:pteridine-containing compound metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02069">
        <property type="entry name" value="methionine_synthase_B12_BD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1240.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.196.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003759">
        <property type="entry name" value="Cbl-bd_cap"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006158">
        <property type="entry name" value="Cobalamin-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036724">
        <property type="entry name" value="Cobalamin-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011005">
        <property type="entry name" value="Dihydropteroate_synth-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033706">
        <property type="entry name" value="Met_synthase_B12-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011822">
        <property type="entry name" value="MetH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036594">
        <property type="entry name" value="Meth_synthase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000489">
        <property type="entry name" value="Pterin-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004223">
        <property type="entry name" value="VitB12-dep_Met_synth_activ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037010">
        <property type="entry name" value="VitB12-dep_Met_synth_activ_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02310">
        <property type="entry name" value="B12-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02607">
        <property type="entry name" value="B12-binding_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02965">
        <property type="entry name" value="Met_synt_B12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00809">
        <property type="entry name" value="Pterin_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000381">
        <property type="entry name" value="MetH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01018">
        <property type="entry name" value="B12-binding_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47644">
        <property type="entry name" value="SSF47644"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51717">
        <property type="entry name" value="SSF51717"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52242">
        <property type="entry name" value="SSF52242"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56507">
        <property type="entry name" value="SSF56507"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02082">
        <property type="entry name" value="metH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50974">
        <property type="entry name" value="ADOMET_ACTIVATION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51332">
        <property type="entry name" value="B12_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51337">
        <property type="entry name" value="B12_BINDING_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50972">
        <property type="entry name" value="PTERIN_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="5 7" id="KW-0846">Cobalamin</keyword>
    <keyword evidence="4 5 6" id="KW-0170">Cobalt</keyword>
    <keyword evidence="5 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0486">Methionine biosynthesis</keyword>
    <keyword evidence="5 8 13" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 5 7" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="5 8 13" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Pterin-binding" evidence="9">
        <location>
            <begin position="27"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="domain" description="B12-binding N-terminal" evidence="12">
        <location>
            <begin position="323"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="domain" description="B12-binding" evidence="11">
        <location>
            <begin position="430"/>
            <end position="569"/>
        </location>
    </feature>
    <feature type="domain" description="AdoMet activation" evidence="10">
        <location>
            <begin position="584"/>
            <end position="915"/>
        </location>
    </feature>
    <feature type="region of interest" description="Methylcob(III)alamin binding" evidence="7">
        <location>
            <begin position="440"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="region of interest" description="S-adenosyl-L-methionine binding" evidence="7">
        <location>
            <begin position="882"/>
            <end position="883"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="371"/>
        </location>
        <ligand>
            <name>methylcob(III)alamin</name>
            <dbReference type="ChEBI" id="CHEBI:28115"/>
        </ligand>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="6">
        <location>
            <position position="443"/>
        </location>
        <ligand>
            <name>methylcob(III)alamin</name>
            <dbReference type="ChEBI" id="CHEBI:28115"/>
        </ligand>
        <ligandPart>
            <name>Co</name>
            <dbReference type="ChEBI" id="CHEBI:27638"/>
        </ligandPart>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="488"/>
        </location>
        <ligand>
            <name>methylcob(III)alamin</name>
            <dbReference type="ChEBI" id="CHEBI:28115"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="492"/>
        </location>
        <ligand>
            <name>methylcob(III)alamin</name>
            <dbReference type="ChEBI" id="CHEBI:28115"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="548"/>
        </location>
        <ligand>
            <name>methylcob(III)alamin</name>
            <dbReference type="ChEBI" id="CHEBI:28115"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="634"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="828"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010398"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRNR" id="PIRNR000381"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR000381-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR000381-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00346"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50972"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51332"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51337"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ABF06974.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="915" mass="100927" checksum="0FF9529E3BBF727F" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-10-07" version="68" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LSG4</accession>
    <name>Q1LSG4_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Extra-cytoplasmic Solute Receptor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">bug</name>
        <name evidence="4" type="ordered locus">Rmet_0026</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Periplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the UPF0065 (bug) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF06912.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0026"/>
    <dbReference type="EnsemblBacteria" id="ABF06912">
        <property type="protein sequence ID" value="ABF06912"/>
        <property type="gene ID" value="Rmet_0026"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0026"/>
    <dbReference type="eggNOG" id="COG3181">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045683_0_1_4"/>
    <dbReference type="OMA" id="IVAYPTG"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042597">
        <property type="term" value="C:periplasmic space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005064">
        <property type="entry name" value="BUG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042100">
        <property type="entry name" value="Bug_dom1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42928">
        <property type="entry name" value="PTHR42928"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03401">
        <property type="entry name" value="TctC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017082">
        <property type="entry name" value="YflP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0675">Receptor</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="42"/>
            <end position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF06912.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="363" mass="37862" checksum="DAA744CAE99D778E" modified="2006-05-30" version="1">MAAIRTRPCISRHVPPNPQIPANFPPISRSSPIMVAFRSLPLHAAFIAAAALTASISVPAFAADNYPSRPIRMIVAYPTGGISDAVARALGEKLSVQMGTSVVVENKAGAGGSIGIDAVAKAAPDGYTVGFSSTSPLTLNPHVGHVNYDPQHDVAPVMSVMYSPVLVVATPSFSGKSFQDLIAQAKAKPGSVRWATSGLGTVGHVMLEQIKAKSKTELTMIPYKGAGQQMNDALGGQFEVMSTNASPMLTQHMQAGRLRAIAVGAPKRIDSLPNVPTMDELGYPKANLTSTFGIFAPGKTPPAIINKLNAELNKALAEPDVRDRLLRGGEVPTGGSAAQFGAAIRAESAENARIVKEAGIKID</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-04-26" modified="2022-01-19" version="96" xmlns="http://uniprot.org/uniprot">
    <accession>Q58AL6</accession>
    <name>Q58AL6_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 6">Flagellar basal body rod protein FlgB</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">flgB</name>
        <name evidence="8" type="ordered locus">Rmet_5971</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="8 9">
        <name>pMOL30</name>
    </geneLocation>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="9">Plasmid pMOL30</plasmid>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5 6">Structural component of flagellum, the bacterial motility apparatus. Part of the rod structure of flagellar basal body.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">The basal body constitutes a major portion of the flagellar organelle and consists of a number of rings mounted on a central rod.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Bacterial flagellum basal body</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the flagella basal body rod proteins family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000354">
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        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="EnsemblBacteria" id="ABF12830">
        <property type="protein sequence ID" value="ABF12830"/>
        <property type="gene ID" value="Rmet_5971"/>
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    <dbReference type="KEGG" id="rme:Rmet_5971"/>
    <dbReference type="HOGENOM" id="CLU_125463_1_0_4"/>
    <dbReference type="OMA" id="GHMARND"/>
    <dbReference type="OrthoDB" id="1914857at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid pMOL30"/>
    </dbReference>
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        <property type="term" value="C:bacterial-type flagellum basal body, rod"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071973">
        <property type="term" value="P:bacterial-type flagellum-dependent cell motility"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="Flag_bb_rod_N"/>
    </dbReference>
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        <property type="entry name" value="FlgB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30435:SF12">
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01396">
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        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="8" id="KW-0969">Cilium</keyword>
    <keyword evidence="8" id="KW-0282">Flagellum</keyword>
    <keyword evidence="8" id="KW-0614">Plasmid</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Flg_bb_rod" evidence="7">
        <location>
            <begin position="16"/>
            <end position="44"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009677"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00014376"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023143"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00024934"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR002889"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF00460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ABF12830.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="140" mass="16115" checksum="D0AB8490A2C3B58E" modified="2005-04-26" version="1">MDTPYVAKWARSEDSVHEQALRLRTRRFELLSANIANADTPNYKARDIDFSAELDRVMGSGQNFAGMTMTSPRHIEASKPALEEDLMYRVPLQSSMDGNTVEMDVERVAFAENALRMRFSIQKTADEYKDMLKLYQDMRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-04-26" modified="2021-06-02" version="53" xmlns="http://uniprot.org/uniprot">
    <accession>Q58AR7</accession>
    <name>Q58AR7_CUPMC</name>
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            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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    </gene>
    <organism evidence="1 2">
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        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
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            <taxon>Betaproteobacteria</taxon>
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        </lineage>
    </organism>
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        <name>pMOL30</name>
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    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
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                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
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                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid pMOL30</plasmid>
        </source>
    </reference>
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    </dbReference>
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    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_006466.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADC45351">
        <property type="protein sequence ID" value="ADC45351"/>
        <property type="gene ID" value="Rmet_6356"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6356"/>
    <dbReference type="HOGENOM" id="CLU_2804132_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid pMOL30"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADC45351.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="73" mass="8092" checksum="53E70160B6C601BE" modified="2005-04-26" version="1">MDNELELRTMVGEADRTLLVAGLQALHRERLAAYNAAVYVAFIRGELHPPMAMFGLDEVHTMLRRVGAAPAPF</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-03-23" modified="2020-10-07" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>D3DXT0</accession>
    <name>D3DXT0_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_6492</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
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        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
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                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
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    </dbReference>
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        <property type="gene ID" value="Rmet_6492"/>
    </dbReference>
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    </dbReference>
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    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADC45100.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="40" mass="4075" checksum="3190235F7F9ED922" modified="2010-03-23" version="1">MSRGGQAASFIHGIAQRLCDNNTIIEGVINGIGATGTSSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-03-23" modified="2020-10-07" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>D3DXX6</accession>
    <name>D3DXX6_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_6539</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
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        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ADC45146.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="protein sequence ID" value="ADC45146"/>
        <property type="gene ID" value="Rmet_6539"/>
    </dbReference>
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    <dbReference type="HOGENOM" id="CLU_3390997_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADC45146.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="32" mass="3683" checksum="25DFD01DC6A5D29C" modified="2010-03-23" version="1">MWGAELVDPIMGPNAQPNWLVLLCKDDWLILM</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-03-23" modified="2021-06-02" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>D3DY14</accession>
    <name>D3DY14_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_6583</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
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        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ADC45184.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="protein sequence ID" value="ADC45184"/>
        <property type="gene ID" value="Rmet_6583"/>
    </dbReference>
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    <dbReference type="HOGENOM" id="CLU_3358006_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="9"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADC45184.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="36" mass="4104" checksum="84FC08C4F999C044" modified="2010-03-23" version="1">MPPPTTATWKREEEFEDMEEVTVRDTDAAGPDIPEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-03-23" modified="2020-12-02" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>D3DY19</accession>
    <name>D3DY19_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Rmet_6588</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
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        <lineage>
            <taxon>Bacteria</taxon>
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    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
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                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ADC45189.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_6588"/>
    <dbReference type="EnsemblBacteria" id="ADC45189">
        <property type="protein sequence ID" value="ADC45189"/>
        <property type="gene ID" value="Rmet_6588"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6588"/>
    <dbReference type="eggNOG" id="COG5567">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_172405_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032831">
        <property type="entry name" value="LPAM_2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13627">
        <property type="entry name" value="LPAM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0564">Palmitate</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="27"/>
            <end position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ADC45189.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="85" mass="8458" checksum="E57435D4CE36BE7D" modified="2010-03-23" version="1">MLRRAAIVSAFAATLAMPLAGCGIRGPLYMPTVPPAPTAPTVADPGLGKPDAPGAVEPARPMSEPSTASPVPVEPARDSSAPSAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-03-23" modified="2020-02-26" version="16" xmlns="http://uniprot.org/uniprot">
    <accession>D3DY60</accession>
    <name>D3DY60_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_6630</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ADC45230.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADC45230">
        <property type="protein sequence ID" value="ADC45230"/>
        <property type="gene ID" value="Rmet_6630"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6630"/>
    <dbReference type="HOGENOM" id="CLU_2957383_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADC45230.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="59" mass="6148" checksum="09619A0F66024FA1" modified="2010-03-23" version="1">MTGATTCNVVAVVVEPVLVEVDPDDVPVVALELPSPPQPARLTAATPMVNDVRNEIAPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="111" xmlns="http://uniprot.org/uniprot">
    <accession>Q1L9Z3</accession>
    <name>Q1L9Z3_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 16 19">Mercuric reductase</fullName>
            <ecNumber evidence="5 16 19">1.16.1.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15 16 19">Hg(II) reductase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19 21" type="primary">merA</name>
        <name evidence="21" type="ordered locus">Rmet_6174</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="21 22">
        <name>pMOL30</name>
    </geneLocation>
    <reference evidence="22" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="22">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="22">Plasmid pMOL30</plasmid>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2 16">Resistance to Hg(2+) in bacteria appears to be governed by a specialized system which includes mercuric reductase. MerA protein is responsible for volatilizing mercury as Hg(0).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 16 19">
            <text>H(+) + Hg + NADP(+) = Hg(2+) + NADPH</text>
            <dbReference type="Rhea" id="RHEA:23856"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16170"/>
            <dbReference type="ChEBI" id="CHEBI:16793"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.16.1.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="16 17 19">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
        <text evidence="16 17 19">Binds 1 FAD per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4 16 19">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 16 19">Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family.</text>
    </comment>
    <dbReference type="EC" id="1.16.1.1" evidence="5 16 19"/>
    <dbReference type="EMBL" id="CP000354">
        <property type="protein sequence ID" value="ABF13033.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011514825.1">
        <property type="nucleotide sequence ID" value="NC_007971.2"/>
    </dbReference>
    <dbReference type="SMR" id="Q1L9Z3"/>
    <dbReference type="EnsemblBacteria" id="ABF13033">
        <property type="protein sequence ID" value="ABF13033"/>
        <property type="gene ID" value="Rmet_6174"/>
    </dbReference>
    <dbReference type="GeneID" id="60820420"/>
    <dbReference type="KEGG" id="rme:Rmet_6174"/>
    <dbReference type="HOGENOM" id="CLU_016755_1_1_4"/>
    <dbReference type="OMA" id="PVDIQMR"/>
    <dbReference type="OrthoDB" id="267896at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid pMOL30"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016152">
        <property type="term" value="F:mercury (II) reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045340">
        <property type="term" value="F:mercury ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016668">
        <property type="term" value="F:oxidoreductase activity, acting on a sulfur group of donors, NAD(P) as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045454">
        <property type="term" value="P:cell redox homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050787">
        <property type="term" value="P:detoxification of mercury ion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00371">
        <property type="entry name" value="HMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.390.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023753">
        <property type="entry name" value="FAD/NAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016156">
        <property type="entry name" value="FAD/NAD-linked_Rdtase_dimer_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017969">
        <property type="entry name" value="Heavy-metal-associated_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006121">
        <property type="entry name" value="HMA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036163">
        <property type="entry name" value="HMA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021179">
        <property type="entry name" value="Mercury_reductase_MerA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001100">
        <property type="entry name" value="Pyr_nuc-diS_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004099">
        <property type="entry name" value="Pyr_nucl-diS_OxRdtase_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012999">
        <property type="entry name" value="Pyr_OxRdtase_I_AS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00403">
        <property type="entry name" value="HMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07992">
        <property type="entry name" value="Pyr_redox_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02852">
        <property type="entry name" value="Pyr_redox_dim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000350">
        <property type="entry name" value="Mercury_reductase_MerA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="SSF51905"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55008">
        <property type="entry name" value="SSF55008"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55424">
        <property type="entry name" value="SSF55424"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02053">
        <property type="entry name" value="MerA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01047">
        <property type="entry name" value="HMA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50846">
        <property type="entry name" value="HMA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00076">
        <property type="entry name" value="PYRIDINE_REDOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 16 17" id="KW-0274">FAD</keyword>
    <keyword evidence="8 16 19" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="7 16" id="KW-0475">Mercuric resistance</keyword>
    <keyword evidence="12 16 19" id="KW-0476">Mercury</keyword>
    <keyword evidence="9 16 19" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="17" id="KW-0520">NAD</keyword>
    <keyword evidence="11 16 19" id="KW-0521">NADP</keyword>
    <keyword evidence="17" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13 16 19" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="21" id="KW-0614">Plasmid</keyword>
    <keyword evidence="14" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HMA" evidence="20">
        <location>
            <begin position="1"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="145"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <begin position="277"/>
            <end position="284"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="362"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="403"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="disulfide bond" description="Redox-active" evidence="18">
        <location>
            <begin position="136"/>
            <end position="141"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000896"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00003340"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012661"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00014791"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00031725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRNR" id="PIRNR000350"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRSR" id="PIRSR000350-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PIRSR" id="PIRSR000350-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="RuleBase" id="RU361223"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="ABF13033.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="561" mass="58698" checksum="8E8AAFD7CD33BCA4" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-10-07" version="37" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LA29</accession>
    <name>Q1LA29_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_6138</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="1 2">
        <name>pMOL30</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid pMOL30</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000354">
        <property type="protein sequence ID" value="ABF12997.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF12997">
        <property type="protein sequence ID" value="ABF12997"/>
        <property type="gene ID" value="Rmet_6138"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6138"/>
    <dbReference type="HOGENOM" id="CLU_2397472_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid pMOL30"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF12997.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="93" mass="10923" checksum="AE9947A2CFB7C08A" modified="2006-05-30" version="1">MLSAATSDNMHGGAAFFQQMRTMSWIRRRQSTPRLWRLVIADFRDFLDSAAPDQCAQRFRQPGLGQLWFRSSWAMRPIARQHDRFKGHRTAVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-10-07" version="55" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LAB5</accession>
    <name>Q1LAB5_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Conjugal transfer protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_6052</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3 4">
        <name>pMOL30</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid pMOL30</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000354">
        <property type="protein sequence ID" value="ABF12911.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF12911">
        <property type="protein sequence ID" value="ABF12911"/>
        <property type="gene ID" value="Rmet_6052"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6052"/>
    <dbReference type="HOGENOM" id="CLU_1049186_0_0_4"/>
    <dbReference type="OMA" id="RRAPMEH"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid pMOL30"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="58"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="83"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="140"/>
            <end position="202"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF12911.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="265" mass="29913" checksum="EED8AC1D12BD4733" modified="2006-05-30" version="1">MAHFPGTRRKPANLSRGAAPMDKFVQIIIAGANIAGCSSSLSRPDYRARVQQMKAKRWMCYAMFYAVAICVVITVKANPGHGYWWAPPLLYALMSWPFFMWYYCGKFQFTPHYVEPIELRCDETQLPCIEVERPVRRRGPSSLELLRRAPMEHQRQPSPPEARTPKRVPAAQRPATLPTPEMADKPMNGKRASVSKHSVPTVPTGLPGAAAAMTDHAEETTDAAKDYWQAKLHRQARHPDEFSLPLFPEEERALNANISSEDPFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LAH2</accession>
    <name>Q1LAH2_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">5-carboxymethyl-2-hydroxymuconate delta-isomerase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">mhpD</name>
        <name evidence="2" type="ordered locus">Rmet_5995</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2 3">
        <name>pMOL30</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid pMOL30</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000354">
        <property type="protein sequence ID" value="ABF12854.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011514815.1">
        <property type="nucleotide sequence ID" value="NC_007971.2"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF12854">
        <property type="protein sequence ID" value="ABF12854"/>
        <property type="gene ID" value="Rmet_5995"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5995"/>
    <dbReference type="HOGENOM" id="CLU_028458_3_1_4"/>
    <dbReference type="OMA" id="EEPDFFI"/>
    <dbReference type="OrthoDB" id="775108at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid pMOL30"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016853">
        <property type="term" value="F:isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.850.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011234">
        <property type="entry name" value="Fumarylacetoacetase-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036663">
        <property type="entry name" value="Fumarylacetoacetase_C_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01557">
        <property type="entry name" value="FAA_hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56529">
        <property type="entry name" value="SSF56529"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0413">Isomerase</keyword>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAA_hydrolase" evidence="1">
        <location>
            <begin position="76"/>
            <end position="291"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF12854.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="292" mass="31914" checksum="11869CED36E189A7" modified="2006-05-30" version="1">MKFATLQTAQGKLVSLVNTESQQYWPLQDLVSGFTGDMVQLVHVFPEIKGRLTPTGEGKPLSQATVLAPIDQPRRNIFCVGKNYHEHAKEFSKSGFDSSAKEGEHAPEAPVVFTKPASTVIGPGKKVPSHPNVTKQLDYEAELAVVIGKAGRAIRKEEAYKHVIGYTIVNDFTARDLQKTHRQWFLGKSLDGFCPMGPYLVTADELDVENLEVKCWINDELRQDSNTSQLIFDIPTLIETLSAGIELQPGDVIATGTPAGVGIGFNPPKFVKAGDVMRIEITGLGTLVNEVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LAY1</accession>
    <name>Q1LAY1_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Acyl carrier protein-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_5836</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12695.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008650026.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5836"/>
    <dbReference type="EnsemblBacteria" id="ABF12695">
        <property type="protein sequence ID" value="ABF12695"/>
        <property type="gene ID" value="Rmet_5836"/>
    </dbReference>
    <dbReference type="GeneID" id="60823581"/>
    <dbReference type="KEGG" id="rme:Rmet_5836"/>
    <dbReference type="eggNOG" id="COG0236">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_108696_10_0_4"/>
    <dbReference type="OMA" id="LATVHVM"/>
    <dbReference type="OrthoDB" id="1943389at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1200.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036736">
        <property type="entry name" value="ACP-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009081">
        <property type="entry name" value="PP-bd_ACP"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00550">
        <property type="entry name" value="PP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47336">
        <property type="entry name" value="SSF47336"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50075">
        <property type="entry name" value="CARRIER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0596">Phosphopantetheine</keyword>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Carrier" evidence="2">
        <location>
            <begin position="1"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="modified residue" description="O-(pantetheine 4'-phosphoryl)serine" evidence="1">
        <location>
            <position position="35"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00258"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF12695.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="83" mass="8995" checksum="F65DA82C0D5F7D1D" modified="2006-05-30" version="1">MKATIRNILGEVARLDVPLATLADNDDLYAAGLSSLATVHVMLALENAFDIEIPDQMLTRQLFRSVDSLAAAVEAIRQQQEAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LAY2</accession>
    <name>Q1LAY2_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="9">UDP-sugar lipid carrier transferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">Rmet_5835</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="10">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="10">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the bacterial sugar transferase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12694.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518045.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5835"/>
    <dbReference type="EnsemblBacteria" id="ABF12694">
        <property type="protein sequence ID" value="ABF12694"/>
        <property type="gene ID" value="Rmet_5835"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5835"/>
    <dbReference type="eggNOG" id="COG2148">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024920_0_1_4"/>
    <dbReference type="OMA" id="NWSVLFD"/>
    <dbReference type="OrthoDB" id="1759731at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <property type="entry name" value="Bact_transf"/>
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        <property type="entry name" value="Undecaprenyl-P_gluc_Ptfrase"/>
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        <property type="match status" value="1"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
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        <source>
            <dbReference type="Pfam" id="PF02397"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="ABF12694.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="468" mass="52648" checksum="C114C23BBD884F67" modified="2006-05-30" version="1">MASEWNVAAERHSALHYLYRLGDAVLIAVCGIAMGALYFHDGIRSAAPVNGFLTMLCALGALLIFPAFGIYESWRGRSRAVLGLRIAAAWTLVFISGLLGAFLIHQIGAVSRVWSMGWFIGTGVMLALSRVVMFRMLANVRQQGINAKRVLIFGYGPLGREMYDRVQHFRDAGYRVVGVYDEESDAIPYDVTPLRQIEEVSGFVRDHGVREIWLTLPMAACRDLSGVVRQFRNEMIDIRWVPDVSSVELLGHRFSEFMGLPVIDLNSPPVSGITGVVKASFDRVFSLGVLVCLSPLLLLIAAMVKLSSPGPVLFRQQRLGIDGQPFNVYKFRTMRQHQDGGGVTQAVRGDPRVTRVGAFLRRTSLDELPQFINVLRGEMSVVGPRPHAMEHNELYKELIDRYMLRHRVKPGITGWAQINGLRGQTDTVEKMRKRIEFDIYYIQHWSFQLDLRIILRTALHGWTGTTAY</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="57" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LB33</accession>
    <name>Q1LB33_CUPMC</name>
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        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_5784</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
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        </lineage>
    </organism>
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    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12643.1"/>
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    <dbReference type="STRING" id="266264.Rmet_5784"/>
    <dbReference type="EnsemblBacteria" id="ABF12643">
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        <property type="gene ID" value="Rmet_5784"/>
    </dbReference>
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    <dbReference type="HOGENOM" id="CLU_2384053_0_0_4"/>
    <dbReference type="OrthoDB" id="2103344at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193026" evidence="2">
        <location>
            <begin position="27"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="58"/>
            <end position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF12643.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="92" mass="9438" checksum="9983DD7F0421D099" modified="2006-05-30" version="1">MKRDCGVRVMTAAWLLAAASSGNALAQHVVGGMGEPVRMGAFDARGQFEGQAILAAPVSGKEKARARPTGTRRVPAAGTRGVTPTLPGKQLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LBF7</accession>
    <name>Q1LBF7_CUPMC</name>
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        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name evidence="7" type="ordered locus">Rmet_5660</name>
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        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
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        </lineage>
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    </geneLocation>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
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                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
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                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
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            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="8">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
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    </dbReference>
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        <property type="gene ID" value="Rmet_5660"/>
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    <dbReference type="OrthoDB" id="1635706at2"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
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        <property type="entry name" value="EAL_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035919">
        <property type="entry name" value="EAL_sf"/>
    </dbReference>
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        <property type="entry name" value="GGDEF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029787">
        <property type="entry name" value="Nucleotide_cyclase"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="PAS-assoc_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035965">
        <property type="entry name" value="PAS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
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        <property type="match status" value="2"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SSF55785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00254">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00229">
        <property type="entry name" value="sensory_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51371">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50883">
        <property type="entry name" value="EAL"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50113">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50112">
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0129">CBS domain</keyword>
    <keyword evidence="7" id="KW-0614">Plasmid</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CBS" evidence="6">
        <location>
            <begin position="14"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="6">
        <location>
            <begin position="77"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="6">
        <location>
            <begin position="204"/>
            <end position="262"/>
        </location>
    </feature>
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        <location>
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            <end position="325"/>
        </location>
    </feature>
    <feature type="domain" description="PAC" evidence="3">
        <location>
            <begin position="352"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="domain" description="GGDEF" evidence="5">
        <location>
            <begin position="436"/>
            <end position="569"/>
        </location>
    </feature>
    <feature type="domain" description="EAL" evidence="4">
        <location>
            <begin position="578"/>
            <end position="832"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABF12519.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="839" mass="92869" checksum="40CE3009F17F5561" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="70" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LBG3</accession>
    <name>Q1LBG3_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_5654</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12513.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008641502.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5654"/>
    <dbReference type="EnsemblBacteria" id="ABF12513">
        <property type="protein sequence ID" value="ABF12513"/>
        <property type="gene ID" value="Rmet_5654"/>
    </dbReference>
    <dbReference type="GeneID" id="60824468"/>
    <dbReference type="KEGG" id="rme:Rmet_5654"/>
    <dbReference type="eggNOG" id="COG2084">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_136595_2_1_4"/>
    <dbReference type="OMA" id="WKIANKA"/>
    <dbReference type="OrthoDB" id="1702133at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032710">
        <property type="entry name" value="NTF2-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039437">
        <property type="entry name" value="Put_lumazine-bd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12893">
        <property type="entry name" value="Lumazine_bd_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54427">
        <property type="entry name" value="SSF54427"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF12513.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="129" mass="14427" checksum="1593F82A8181DCB4" modified="2006-05-30" version="1">MTNQAIPAEYLAALEPIQHYLEAHRTGRAEYVRKAFHADARIISFRDGTLHSLTVEEFSARFQGEPAADEAQRRRFIASFDVVGNAATAKVVLQYPEVTFTDYMNLLQIDGVWKIANKTFSAAPKAPAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="92" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LCA9</accession>
    <name>Q1LCA9_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Putative Acyl-CoA dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Rmet_5358</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="6">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="6">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the acyl-CoA dehydrogenase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12217.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519763.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5358"/>
    <dbReference type="EnsemblBacteria" id="ABF12217">
        <property type="protein sequence ID" value="ABF12217"/>
        <property type="gene ID" value="Rmet_5358"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5358"/>
    <dbReference type="eggNOG" id="COG1960">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018204_5_0_4"/>
    <dbReference type="OMA" id="AKIRCAE"/>
    <dbReference type="OrthoDB" id="760677at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016627">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-CH group of donors"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.540.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036250">
        <property type="entry name" value="AcylCo_DH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009075">
        <property type="entry name" value="AcylCo_DH/oxidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013786">
        <property type="entry name" value="AcylCoA_DH/ox_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037069">
        <property type="entry name" value="AcylCoA_DH/ox_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009100">
        <property type="entry name" value="AcylCoA_DH/oxidase_NM_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00441">
        <property type="entry name" value="Acyl-CoA_dh_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02771">
        <property type="entry name" value="Acyl-CoA_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47203">
        <property type="entry name" value="SSF47203"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56645">
        <property type="entry name" value="SSF56645"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0274">FAD</keyword>
    <keyword evidence="2" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="5" id="KW-0614">Plasmid</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Acyl-CoA_dh_N" evidence="4">
        <location>
            <begin position="3"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA_dh_1" evidence="3">
        <location>
            <begin position="197"/>
            <end position="309"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF02771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABF12217.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="346" mass="37238" checksum="769D88D1D959E884" modified="2006-05-30" version="1">MNELEQMLTDSAERLFGNEVTLEQSEAMERGEFPQSLWNAVDKSGLVHVLASEEAGGANASWTEALPVMLAAGRTVTPLPFVDAATATWLLSRLGIEAPQGLLALADLSAAPTASQDGDTWRLSAEVEVPWGRHAQHVLVRAANAWLLLPTEHASVREDANIAGEPRDRLTFVDAVATAASTRQPLEGCDDPAALGALMRAIQITGVLERVLNQTVQYATERIQFGRPIGKFQAIQQQLAVLANEVVASRMAVASACAALSGKDWAQQAMIAKIICGLAAGRAPAIAHQVHGAIGFTREHSLHYATRRLWSWRAEYGSEAQWAAKLGKMAIEHGGDTLWERLTEAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LCD2</accession>
    <name>Q1LCD2_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative metallo-beta-lactamase putative exported protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_5335</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12194.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519737.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5335"/>
    <dbReference type="EnsemblBacteria" id="ABF12194">
        <property type="protein sequence ID" value="ABF12194"/>
        <property type="gene ID" value="Rmet_5335"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5335"/>
    <dbReference type="eggNOG" id="COG0491">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_056519_0_0_4"/>
    <dbReference type="OMA" id="GMQTAVN"/>
    <dbReference type="OrthoDB" id="1815576at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001279">
        <property type="entry name" value="Metallo-B-lactamas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00753">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00849">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="SSF56281"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Lactamase_B" evidence="1">
        <location>
            <begin position="110"/>
            <end position="321"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF12194.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="351" mass="37933" checksum="AC073080BD9EC20A" modified="2006-05-30" version="1">MKLHSAPLRAGMGSGRRVFQTLVSATLLAVAALVLPAMPTDARAQAPAHAQKKTQVPGYYRMMVGQFEVTALYDGYIDIDPKLFKYTSAREVQRLLARMFVESTPGMQTAVNAYLVNTGSHLILVDTGASACFGPTLGRIRENLHAAGYEPEQVDTILLTHLHADHACGLLSNGTAVFPAATVYADKADADYWLDESKAAAAPKDAQGLFAMARDAVAPYRAAGRFRTFSASASAGAGDELVPGVRAVPEFGHTPGHVGYLFTSGKEKLLLWGDVIHSHAVQFRSPQVAIEFDTDSHQAVITRNRVLADAARDKLWVGGAHLPFPGIGHVRREQVGYTWVPVEYGPIRNDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LCM3</accession>
    <name>Q1LCM3_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Major facilitator superfamily protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Rmet_5244</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="8">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="8">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12103.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519649.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5244"/>
    <dbReference type="EnsemblBacteria" id="ABF12103">
        <property type="protein sequence ID" value="ABF12103"/>
        <property type="gene ID" value="Rmet_5244"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5244"/>
    <dbReference type="eggNOG" id="COG0477">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000960_22_3_4"/>
    <dbReference type="OMA" id="MQITMLV"/>
    <dbReference type="OrthoDB" id="1558246at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="SSF103473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0614">Plasmid</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="21"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="56"/>
            <end position="74"/>
        </location>
    </feature>
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        </location>
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            <begin position="147"/>
            <end position="168"/>
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            <begin position="174"/>
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            <end position="225"/>
        </location>
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        <location>
            <begin position="237"/>
            <end position="255"/>
        </location>
    </feature>
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        <location>
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            <end position="291"/>
        </location>
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        <location>
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        <location>
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        <location>
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            <end position="491"/>
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        <location>
            <begin position="21"/>
            <end position="499"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABF12103.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="513" mass="53795" checksum="260AE8D6E95E1B66" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="99" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LCW5</accession>
    <name>Q1LCW5_CUPMC</name>
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        <submittedName>
            <fullName evidence="8">Putative acyl-CoA dehydrogenase</fullName>
            <ecNumber evidence="8">1.3.8.8</ecNumber>
            <ecNumber evidence="8">1.3.99.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Rmet_5152</name>
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        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="9">
        <name>megaplasmid CH34</name>
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    <reference evidence="9" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="9">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the acyl-CoA dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.3.8.8" evidence="8"/>
    <dbReference type="EC" id="1.3.99.-" evidence="8"/>
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        <property type="protein sequence ID" value="ABF12011.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519557.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5152"/>
    <dbReference type="EnsemblBacteria" id="ABF12011">
        <property type="protein sequence ID" value="ABF12011"/>
        <property type="gene ID" value="Rmet_5152"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5152"/>
    <dbReference type="eggNOG" id="COG1960">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018204_0_3_4"/>
    <dbReference type="OMA" id="NGTHCDL"/>
    <dbReference type="OrthoDB" id="760677at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004466">
        <property type="term" value="F:long-chain-acyl-CoA dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.540.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.110.10">
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Acyl-CoA_DH_CS"/>
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    <dbReference type="InterPro" id="IPR006091">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046373">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom_sf"/>
    </dbReference>
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        <property type="entry name" value="AcylCo_DH-like_C"/>
    </dbReference>
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        <property type="entry name" value="AcylCo_DH/oxidase_C"/>
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        <property type="entry name" value="AcylCoA_DH/ox_N"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="ACYL_COA_DH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="3" id="KW-0285">Flavoprotein</keyword>
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    </feature>
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    </evidence>
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    </evidence>
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    </evidence>
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    </evidence>
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    </evidence>
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            <dbReference type="Pfam" id="PF02771"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
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    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="63" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LCY1</accession>
    <name>Q1LCY1_CUPMC</name>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_5136</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11995.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5136"/>
    <dbReference type="EnsemblBacteria" id="ABF11995">
        <property type="protein sequence ID" value="ABF11995"/>
        <property type="gene ID" value="Rmet_5136"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5136"/>
    <dbReference type="eggNOG" id="ENOG502ZAFN">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1124155_0_0_4"/>
    <dbReference type="OMA" id="ALMACRG"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF11995.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="261" mass="29319" checksum="0507DA15FF38231A" modified="2006-05-30" version="1">MRDDRDPRITLQPMSRSHPQPHLQDSLTAYYWSGDAIRSRRVSDVVLSGTVDIPEPPARLTADWAREISHHMNLEVGDVEVMPLARARVRWPDYSRCVRAVSDWASTLGLPEVLAASDVALMVCRGARYHHDGDQYGGAAFCNLFLSEDKGQDVHFPLLDLRIPLQRGSVLIFDTCQPHAVIPRGSSRFSAADFPANQDCTQLFLTWELPIENAHVARALKIAFDSDGSTARDLDEEQVRLHCERVGVCQETGAWQRYVAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-10-07" version="53" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LD63</accession>
    <name>Q1LD63_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_5051</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
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        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11913.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5051"/>
    <dbReference type="EnsemblBacteria" id="ABF11913">
        <property type="protein sequence ID" value="ABF11913"/>
        <property type="gene ID" value="Rmet_5051"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5051"/>
    <dbReference type="HOGENOM" id="CLU_972780_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193074" evidence="2">
        <location>
            <begin position="22"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="135"/>
            <end position="154"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF11913.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="286" mass="28030" checksum="4D98033584FB223B" modified="2006-05-30" version="1">MTKTRRFAAATLYGFLAVTIAACGGGGDAAGTGSATDATPAASCTAAKPQCTVSSSPATSADNPVPAGTNVVLTAKCDTSVTQADWFTNAKTFASGMNVTVNPSATTSYGFAYKNCAGGDATGLWVYVGSGSSSGSSSGGSAGTSTGTGGSATVDSGASGSGGLKYGVSAMNCVRMGHNASGSTTITNTCGGTISYTFCNLYAAPDGDTGSLFACKGQRATFSPSGYVYGQGADSLGAGNTQVILGTRVNNQVTWVIACATGLPYITNFDTSTIGPNSKATGICVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="95" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LDI6</accession>
    <name>Q1LDI6_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Lysine-specific permease amino acid-polyamine-organocation (APC) superfamily</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">lysP2</name>
        <name evidence="8" type="ordered locus">Rmet_4928</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="9">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="9">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11790.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519350.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4928"/>
    <dbReference type="EnsemblBacteria" id="ABF11790">
        <property type="protein sequence ID" value="ABF11790"/>
        <property type="gene ID" value="Rmet_4928"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4928"/>
    <dbReference type="eggNOG" id="COG0833">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007946_12_1_4"/>
    <dbReference type="OMA" id="AMFSTAN"/>
    <dbReference type="OrthoDB" id="430372at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006865">
        <property type="term" value="P:amino acid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004841">
        <property type="entry name" value="AA-permease/SLC12A_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004840">
        <property type="entry name" value="Amoino_acid_permease_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00324">
        <property type="entry name" value="AA_permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00218">
        <property type="entry name" value="AMINO_ACID_PERMEASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0614">Plasmid</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="20"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="51"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="104"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="133"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="163"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="204"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="249"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="291"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="348"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="375"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="420"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="451"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="domain" description="AA_permease" evidence="7">
        <location>
            <begin position="23"/>
            <end position="478"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ABF11790.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="510" mass="54998" checksum="477AF8C0F8AF78D2" modified="2006-05-30" version="1">MSNTNTTPAVPVPGLRRELRARHLTMIAIGGSIGTGLFVASGATIAQAGPGGALAAYILIGAMVYFLMTSLGELAAYMPVSGSFATYGALYVDEGFGFALGWNYWYNWAVTIAVELAAAQLVMQYWFPDTPGVLWSALFLGLMFALNAISVRGFGEAEYWCALIKVLTVIAFIVIGTLMIFGIMRGDFSHAGGLANLTTGDAPFVGGLPAMIGVAMIAGFSFQGTELIGVAAGESDEPAKNIPRAVRQVFWRILLFYVLAILIIGILIPYTDPSLLKTDVETVGVSPFTLVFRHAGLAFAAGVMNAVILTAVLSAGNSGMYASTRMLYNLATEGRAPRIFAQLTRNGVPLVALLATTAVGGLCFLTSLFESKSVYLWLLNLSGMTGFIAWLGIAVSHYRFRRGFMAQSLDLNRLPYRSPFFPYGPIFAFVLCMIVTLGQNYQAFSEGKIDWVAVTATYVGIPIFLLIWLGYRLVRKSRFVPYAEMKFPALPEIEKDDSPVTRPLAPASAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LDK1</accession>
    <name>Q1LDK1_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Putative transmembrane component of ABC-type transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">nosY</name>
        <name evidence="7" type="ordered locus">Rmet_4913</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="8">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="8">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11775.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4913"/>
    <dbReference type="EnsemblBacteria" id="ABF11775">
        <property type="protein sequence ID" value="ABF11775"/>
        <property type="gene ID" value="Rmet_4913"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4913"/>
    <dbReference type="eggNOG" id="COG1277">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_071765_1_0_4"/>
    <dbReference type="OMA" id="RWSYVYF"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032688">
        <property type="entry name" value="ABC2_membrane_2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12679">
        <property type="entry name" value="ABC2_membrane_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0614">Plasmid</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="24"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="57"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="109"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="144"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="179"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="250"/>
            <end position="269"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABF11775.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="273" mass="29497" checksum="7913E5C673C3A47D" modified="2006-05-30" version="1">MAIECRQVVTLAAKELRDRLRNRWVLAVATVFAVFSLVICYFGGAEQGTLGPRSLEFVITSLVSLVIYLIPMIALLLGFDAVVGERERGSLDLLLALPVTRFEVLLGKYLGLAVALTLSTLAGFALMVVLLWQQFGWVGLFHAFGFVVSSILLGLVFLSLSLLISVISRDRARASGAAIGLWFGFVLVFDLLMLGLLVASGGSFGGEALAYVLLLNPTDIFRILNVFSLEQLRGLQGLVSIVPPALDNPWAMGGAMLGWIAAPLALAAWRFRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LDM2</accession>
    <name>Q1LDM2_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Sulfite:cytochrome c oxidoreductase subunit B</fullName>
            <ecNumber evidence="7">1.8.2.1</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">sorB</name>
        <name evidence="7" type="ordered locus">Rmet_4892</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="8">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="8">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EC" id="1.8.2.1" evidence="7"/>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11754.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519314.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4892"/>
    <dbReference type="EnsemblBacteria" id="ABF11754">
        <property type="protein sequence ID" value="ABF11754"/>
        <property type="gene ID" value="Rmet_4892"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4892"/>
    <dbReference type="eggNOG" id="COG4654">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1325412_0_0_4"/>
    <dbReference type="OMA" id="APQDIKL"/>
    <dbReference type="OrthoDB" id="1939800at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050310">
        <property type="term" value="F:sulfite dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.760.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009056">
        <property type="entry name" value="Cyt_c-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036909">
        <property type="entry name" value="Cyt_c-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002324">
        <property type="entry name" value="Cyt_c_ID"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13442">
        <property type="entry name" value="Cytochrome_CBB3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00606">
        <property type="entry name" value="CYTCHROMECID"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46626">
        <property type="entry name" value="SSF46626"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51007">
        <property type="entry name" value="CYTC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 4" id="KW-0349">Heme</keyword>
    <keyword evidence="3 4" id="KW-0408">Iron</keyword>
    <keyword evidence="2 4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-0614">Plasmid</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193352" evidence="5">
        <location>
            <begin position="31"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="domain" description="Cytochrome c" evidence="6">
        <location>
            <begin position="134"/>
            <end position="218"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABF11754.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="218" mass="23062" checksum="740FA25109BF88C9" modified="2006-05-30" version="1">MQKTPCSRFPARRLAISLALMLSALPAAHALEIQLPPETASYQPSELPGYRLVQQNCMTCHSAQYVSTQPQSLGRAYWDATVKKMKTAFGAPLRDEDIPVMADYLVRTYGAERQTTAAAAVSPAPAASAKPAPAKAHDASSLLAANNCLACHAVDKKVVGPAFHDVAAKYAGQADAVSTVMRSIRAGGSGKWGSVPMPPFADVSDADLRELAGWVLGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LDX4</accession>
    <name>Q1LDX4_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">RND efflux system, outer membrane lipoprotein, NodT family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Rmet_4790</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="5">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="5">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the outer membrane factor (OMF) (TC 1.B.17) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11652.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519219.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4790"/>
    <dbReference type="EnsemblBacteria" id="ABF11652">
        <property type="protein sequence ID" value="ABF11652"/>
        <property type="gene ID" value="Rmet_4790"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4790"/>
    <dbReference type="eggNOG" id="COG1538">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012817_13_0_4"/>
    <dbReference type="OMA" id="FGPLISW"/>
    <dbReference type="OrthoDB" id="1619477at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015562">
        <property type="term" value="F:efflux transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003423">
        <property type="entry name" value="OMP_efflux"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010131">
        <property type="entry name" value="RND_efflux_OM_lipoprot_NodT"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02321">
        <property type="entry name" value="OEP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01845">
        <property type="entry name" value="outer_NodT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0564">Palmitate</keyword>
    <keyword evidence="4" id="KW-0614">Plasmid</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1134">Transmembrane beta strand</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5001442008" evidence="2">
        <location>
            <begin position="19"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="476"/>
            <end position="496"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU362097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF11652.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="496" mass="53097" checksum="9D5BAE06DE44CD37" modified="2006-05-30" version="1">MKRFARASLAAMLPLALAACMTVGPDHKVPDDAVVKSSAANGPFAGTDSDAVSIGDVPNDWWRLYDDPRIDELVQQALVANTDLRVAAANLKRSIAVYHEVEAENLPETKFRASAERGQIAGEPLLHEEKIPVMNFGDIGLNVSYLIDFWGKLKRADEAALASAQASHAALDQARVGVVAETVRAYVQGCTATHELHVAAHQLDLQERGVKLAQKLVDAGRGQPTDLLRAEAQADTLRSALPKYRAEQEGAAYRLAVMLGKPPTALDVSSVACEHVPALKQALPVGDGMALLKRRPDVRQAERELASATAKIGVATADLFPQIRIGASAGFTGILDHLGQAPTAHWGWGPAISWTIPTSGTRARIHNMEYGAEGALAHFDGVVLKALRETQTALSAYTHELDRNASLRSARDKANEAARQNRLLWQAGRSPYLQSLDADRTLANTDAALAASDAQVAMDQINLFLALGGGWQDAPAIASHPVGKEPGQATEHTEEH</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LE14</accession>
    <name>Q1LE14_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Rh-like protein/ammonium transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">Rmet_4750</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="11">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="11">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ammonia transporter channel (TC 1.A.11.2) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11612.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519184.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4750"/>
    <dbReference type="EnsemblBacteria" id="ABF11612">
        <property type="protein sequence ID" value="ABF11612"/>
        <property type="gene ID" value="Rmet_4750"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4750"/>
    <dbReference type="eggNOG" id="COG0004">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_33_1_4"/>
    <dbReference type="OMA" id="CAGSDVM"/>
    <dbReference type="OrthoDB" id="562287at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008519">
        <property type="term" value="F:ammonium transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3430.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029020">
        <property type="entry name" value="Ammonium/urea_transptr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018047">
        <property type="entry name" value="Ammonium_transpt_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024041">
        <property type="entry name" value="NH4_transpt_AmtB-like_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00909">
        <property type="entry name" value="Ammonium_transp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01219">
        <property type="entry name" value="AMMONIUM_TRANSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0924">Ammonia transport</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0614">Plasmid</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="12"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="47"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="89"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="117"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="157"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="201"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="227"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="256"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="280"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="310"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="341"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="domain" description="Ammonium_transp" evidence="9">
        <location>
            <begin position="12"/>
            <end position="390"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ABF11612.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="400" mass="42145" checksum="C2FB7823B952AA71" modified="2006-05-30" version="1">MDNLKTGTDAMFILLGAIMVLAMHAGFAFLELGTVRKKNQVNALVKILVDFAVSTISYFFIGYTVAYGVQFMSGADVLAQKSGYELVKFFFLATFAAAIPAIISGGIAERSRFNPQLAATFVLVGFVYPFFEGIAWNNRFGIQDWIASVTGAPFHDFAGSVVVHAVGGWIALPAVLLLGARRGRYQKDGRISAHPPSNIPFLALGAWVLAVGWFGFNVMSAQTVDKISGLVAINSLMAMAGGTLAAWWAGRNDPGFAYNGPLAGLVAVCAGSDLMHPLGALITGAVAGVLFVHLFTLAQNKWHIDDVLGVWPLHGLCGAWGGIAAGIFGSHALGGIGGVSIVAQLIGTAMGIVIALVGGTVVYGTLKKVVGIRLDAEEEYNGADLTLHRISATPERETSW</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LE73</accession>
    <name>Q1LE73_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative permease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_4691</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11553.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519124.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4691"/>
    <dbReference type="EnsemblBacteria" id="ABF11553">
        <property type="protein sequence ID" value="ABF11553"/>
        <property type="gene ID" value="Rmet_4691"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4691"/>
    <dbReference type="eggNOG" id="COG2610">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_042638_1_1_4"/>
    <dbReference type="OMA" id="MPFFGTT"/>
    <dbReference type="OrthoDB" id="1585405at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015128">
        <property type="term" value="F:gluconate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003474">
        <property type="entry name" value="Glcn_transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30354">
        <property type="entry name" value="PTHR30354"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02447">
        <property type="entry name" value="GntP_permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="50"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="97"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="135"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="176"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="255"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="298"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="333"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="376"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="460"/>
            <end position="482"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF11553.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="485" mass="50087" checksum="B21D1DDD8579D481" modified="2006-05-30" version="1">MGLLGIVIALGAMIWLAFRGWSILIIAPAAALLAAATAGEPLLAHWTQTFMGTAAGFVAQFFPLFLLGALFGKLMDDSGSVTAIAEWMTRVLGPQRAMLAVVLAGALLTYGGVSLFVAFFVLAPMAHALFRAADIPPRLMPAAIVLGTSTFTMSALPGTPAIQNAIPMPFFSTTPFAAPGLGIVASAIMLTFGLWWLSQRENAARRRGEGYAIAEEEASPDEVAERPLIRELASTAREFDPAEALQGSRSDEPPAAFIALLPLAVVIVVNLLMSFVVLPRVDADYLGLPEWGATTLQAVSGVWSVIVALTAAILCLIALNFRRLPELRDSMGAGAHAAALPVLSVASLVGFGAVVASLPGFGVVRDWVLSIEGGPLVSLAVATNVLSALTGSASGGLTIALDALGGTYLTRAFDLGIDPAILHRVAVMSAGTLDTLPHNGAVVTLLAVCGATHRESYGGIVMVGIVGPMLALAAVIALGSWVGSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LEH1</accession>
    <name>Q1LEH1_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Polysaccharide deacetylase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_4593</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11455.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519036.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4593"/>
    <dbReference type="EnsemblBacteria" id="ABF11455">
        <property type="protein sequence ID" value="ABF11455"/>
        <property type="gene ID" value="Rmet_4593"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4593"/>
    <dbReference type="eggNOG" id="COG0726">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_084199_0_0_4"/>
    <dbReference type="OMA" id="KFLWKML"/>
    <dbReference type="OrthoDB" id="1701876at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016810">
        <property type="term" value="F:hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011330">
        <property type="entry name" value="Glyco_hydro/deAcase_b/a-brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002509">
        <property type="entry name" value="NODB_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01522">
        <property type="entry name" value="Polysacc_deac_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88713">
        <property type="entry name" value="SSF88713"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51677">
        <property type="entry name" value="NODB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NodB homology" evidence="1">
        <location>
            <begin position="2"/>
            <end position="261"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF11455.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="296" mass="32823" checksum="8E2244144D7E6090" modified="2006-05-30" version="1">MARIALKVDVDTLRGTREGVPALLSMLSAAEAEATFLFSLGPDHTGWALRRVFRPGFLKKVSRTSVVSNYGLRTLMYGVLLPGPDIGRKGAAEMRAAAAAGHECGIHTWDHVYWQDNVRERDAAWTRRQMQQSFERFTDIFGAAPTTHGAAGWQMNNEAFRQIDDWGMVYASDGRGTAPYIPTVDGKACRHVQMPTTLPTLDELIGVDDLTEDNVHKAVLKLTEGDGDHIFTLHTELEGGKLAPVFKRLLSGWRAQGHQLVSMATYYQHIDRSDLPQMPVTWGEIPGRSGELIIQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LEH2</accession>
    <name>Q1LEH2_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Epimerase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_4592</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11454.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519035.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4592"/>
    <dbReference type="EnsemblBacteria" id="ABF11454">
        <property type="protein sequence ID" value="ABF11454"/>
        <property type="gene ID" value="Rmet_4592"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4592"/>
    <dbReference type="eggNOG" id="COG0451">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007383_7_0_4"/>
    <dbReference type="OMA" id="WIYSCAK"/>
    <dbReference type="OrthoDB" id="1566711at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016831">
        <property type="term" value="F:carboxy-lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05257">
        <property type="entry name" value="Arna_like_SDR_e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045869">
        <property type="entry name" value="Arna-like_SDR_e"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001509">
        <property type="entry name" value="Epimerase_deHydtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01370">
        <property type="entry name" value="Epimerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Epimerase" evidence="1">
        <location>
            <begin position="4"/>
            <end position="252"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF11454.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="350" mass="39651" checksum="CDE759F94848D899" modified="2006-05-30" version="1">MKKVLILGVNGFIGHHLTRRILETTQWEVYGMDMSSDRLGDLVNHPRMHFFEGDITINKEWIEYNIRKCDVVLPLVAIATPATYVREPLRVFELDFEANLPIVRAAVKYGKHLVFPSTSEVYGMCADEEFDPEASPLIYGPINKPRWIYACSKQLMDRVIHAYGMQEGLNYTLFRPFNWIGAGLDSIFESKEGSSRVVTQFLGHIVRGEPIKLVDGGAQQRAFADISDGISALMRIIENKDGVANGKIFNIGNPGNIHSVRELAEMMLKMAAEYPEYAEEARKTKIVETSSGDFYGKGYQDVQHRVPKIDNTIGELGWKPEVSMEQALRRIFEAYRDKVVEARTLVDADN</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-12-02" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LEN2</accession>
    <name>Q1LEN2_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Sulfatase-modifying factor (C-alpha-formyglycine-generating enzyme) DUF323</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">sumF1</name>
        <name evidence="4" type="ordered locus">Rmet_4529</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="5">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="5">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11394.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4529"/>
    <dbReference type="EnsemblBacteria" id="ABF11394">
        <property type="protein sequence ID" value="ABF11394"/>
        <property type="gene ID" value="Rmet_4529"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4529"/>
    <dbReference type="eggNOG" id="COG1262">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012431_4_0_4"/>
    <dbReference type="OMA" id="RQMANVW"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1580.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016187">
        <property type="entry name" value="CTDL_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005532">
        <property type="entry name" value="SUMF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042095">
        <property type="entry name" value="SUMF_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03781">
        <property type="entry name" value="FGE-sulfatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56436">
        <property type="entry name" value="SSF56436"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0614">Plasmid</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="23"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="domain" description="FGE-sulfatase" evidence="3">
        <location>
            <begin position="67"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="320"/>
            <end position="340"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF03781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF11394.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="408" mass="44491" checksum="CC9E7087B1F5A54C" modified="2006-05-30" version="1">MARKQQERAAAASAAPSSWRRRMWLGTLVVGMAGAGAAGTVWYSRHNGQGVDPASIRAGDGVSGPAGMVHVPGGEFLMGSDHKMSQANERPAHKVQVKAFWMDQHHVTNADFRKFVEATGYLTTAERKPDWETLRVQLPAGTPRPPDSAMVAGGMVFVGTNSPVPLREYWRWWRFVPGADWRHPTGPGSSIEGKDNHPVVQVSYEDAQAYAKWAGKRLPTEAEWEFAARGGLEQATYAWGDKFAPDGRQMANVWQGQQVQPFPVVSAKAGGAAGTSAVGTFPGNGYGLYDMTGNAWQWVADWYRADQFRREATVAAVLQNPTGPADSWDPTEPGVPVSAPKRVTRGGSFLCNEDFCLSYRPSARRGTDPYTSMSHLGFRLVMDDARWAEVRKQPAVAMAAGGQQNVQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="68" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LES2</accession>
    <name>Q1LES2_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Phage head/capsid assembly, phage maturation protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">zot</name>
        <name evidence="2" type="ordered locus">Rmet_4489</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11354.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518952.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4489"/>
    <dbReference type="EnsemblBacteria" id="ABF11354">
        <property type="protein sequence ID" value="ABF11354"/>
        <property type="gene ID" value="Rmet_4489"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4489"/>
    <dbReference type="eggNOG" id="COG4128">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_066220_0_0_4"/>
    <dbReference type="OMA" id="DECGTWF"/>
    <dbReference type="OrthoDB" id="671293at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008900">
        <property type="entry name" value="Zot_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05707">
        <property type="entry name" value="Zot"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Zot" evidence="1">
        <location>
            <begin position="7"/>
            <end position="190"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF05707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF11354.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="358" mass="40525" checksum="D0A64E4BDB354B2F" modified="2006-05-30" version="1">MSDYALTGKKGTGKSSGAVRQIQARLRAGQRVATNLNIHMDHLMPAHSRAFVIRLRDKPSAHDLFSLGSGNPKIKFDPIVDTDPKSKTYGQCIGTTDPQILDGFDENENGLLVLDEMGTWLNSRSFQDASRAPVLDWLAHGRKFGWDVFYLMQHINQVDKQLRESFIEYCVRFHRLDKIKIPGVSAATRLITAGQFKGQFPRFHIGVMRLGCDPAGMVADRWVFRGGNIHKAYDTTQVFQESYPHGTFCYLSHWHLEGWKHPEVVPIGWRALNWMRKFWQPSVGSWSIKDRQLVAAVYAREKSPQKRQKLFEVLARQQAQMNRAGARLVAAGGSLAPTLPSLKDQMEVVRRSLKRGAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="62" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LEV0</accession>
    <name>Q1LEV0_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Protein involved in acute metal response (CopQ/CzcJ/MmrQ/MmtQlike)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">mmmQ</name>
        <name evidence="3" type="ordered locus">Rmet_4461</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11326.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518929.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4461"/>
    <dbReference type="EnsemblBacteria" id="ABF11326">
        <property type="protein sequence ID" value="ABF11326"/>
        <property type="gene ID" value="Rmet_4461"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4461"/>
    <dbReference type="eggNOG" id="ENOG502ZK25">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_136209_5_1_4"/>
    <dbReference type="OrthoDB" id="2228253at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193474" evidence="2">
        <location>
            <begin position="27"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="27"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="42"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF11326.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="88" mass="9216" checksum="CFEB2BA2D57CB19F" modified="2006-05-30" version="1">MRTIKQALLYTALTATLASAAGLANAAPRSADPFTDGARVTNRDGLVERTGTRDPYTDGGRAIHDPRSPYTDGGFSATGKRDVFTDGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-12-02" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LEY3</accession>
    <name>Q1LEY3_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Beta-methylmalyl-CoA dehydratase</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">2-methylfumaryl-CoA hydratase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Mesaconyl-CoA hydratase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_4428</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(2R,3S)-beta-methylmalyl-CoA = 2-methylfumaryl-CoA + H2O</text>
            <dbReference type="Rhea" id="RHEA:38263"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:75634"/>
            <dbReference type="ChEBI" id="CHEBI:75635"/>
            <dbReference type="EC" id="4.2.1.148"/>
        </reaction>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11293.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4428"/>
    <dbReference type="EnsemblBacteria" id="ABF11293">
        <property type="protein sequence ID" value="ABF11293"/>
        <property type="gene ID" value="Rmet_4428"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4428"/>
    <dbReference type="eggNOG" id="COG2030">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_067804_0_0_4"/>
    <dbReference type="OMA" id="RLIYGGH"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016833">
        <property type="term" value="F:oxo-acid-lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029069">
        <property type="entry name" value="HotDog_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002539">
        <property type="entry name" value="MaoC-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016790">
        <property type="entry name" value="Thiol_ester_hydratase_Rv0216"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01575">
        <property type="entry name" value="MaoC_dehydratas"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF021494">
        <property type="entry name" value="Rv0216_prd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54637">
        <property type="entry name" value="SSF54637"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0456">Lyase</keyword>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MaoC-like" evidence="2">
        <location>
            <begin position="17"/>
            <end position="129"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRNR" id="PIRNR021494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01575"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF11293.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="353" mass="38383" checksum="7FAD014FA2AFA979" modified="2006-05-30" version="1">MDMEKCRSGNYFEDFRVGMKIRHATPRTLTEGDRSLYIGLTGSRAALCTAETNARDLGMERRPLEDLLVFNTAFGKTVPDISLNAVANLGYAEVRFLAHVYPGDTLAVESEVIGLKENTSGKSGVVYVRSMARNQHGREVLSWVRWVMVHKRDHGAACAEAVVPATQAVVPSAWMRRDDYTARVREITAMTGFADLWDDYAVGERIEHPGAMTINDSDHSIATRLYQNTARAHFDGHAMAAGGGRRLVYGGHIISVCRALSYDGLENALSILAINGGSHVNPTYAGDTIACATQVIEKLDLGQGHLGGLRLRMIGVKNVSAASIAFPAPGESRTAHPSSVVLDLDYTIAIPRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-12-02" version="64" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LFD3</accession>
    <name>Q1LFD3_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_4277</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11143.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4277"/>
    <dbReference type="EnsemblBacteria" id="ABF11143">
        <property type="protein sequence ID" value="ABF11143"/>
        <property type="gene ID" value="Rmet_4277"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4277"/>
    <dbReference type="eggNOG" id="ENOG5033A7D">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_188292_3_0_4"/>
    <dbReference type="OMA" id="LYRFFWH"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012451">
        <property type="entry name" value="DUF1656"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07869">
        <property type="entry name" value="DUF1656"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="13"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="49"/>
            <end position="73"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF11143.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="74" mass="8360" checksum="935BEB9367B11179" modified="2006-05-30" version="1">MTQTGTTAMPREIAFFSVLMPSLLPVFLACGVLYWIFDHLLARAGIYRLVWHPALFRVALFACLFSGIGLLLWD</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LFL2</accession>
    <name>Q1LFL2_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Short-chain dehydrogenase/reductase SDR</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_4197</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11064.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4197"/>
    <dbReference type="EnsemblBacteria" id="ABF11064">
        <property type="protein sequence ID" value="ABF11064"/>
        <property type="gene ID" value="Rmet_4197"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4197"/>
    <dbReference type="eggNOG" id="COG1028">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010194_17_0_4"/>
    <dbReference type="OMA" id="DTAFIRD"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF11064.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="298" mass="33250" checksum="7040C1A8E150751A" modified="2006-05-30" version="1">MHLTIYIVRYKVKDEFSGARYGNVPVIMQATTQTPRTPKAFHAVPFPQFNLAGGDTVPEKKAILVVGAGDATGGAIARRFAHEGYVACVTRRDVDKLAPLVEQIRAEGGEAHAFGCDARKEEETVAMIERIERDIGQLEVAVFNIGANVNFPILETTSRVYFKVWEMACFAGFLMGREAARVMVPRQRGSIFFTGATASTRGREGMSAFSGAKHALRALAQSMARELGPKNLHVAHLIIDAAIDTAFIRDNFPDRYATRTQDGIVNPEHIADTYWMLHQQPRDAWTHELDLRPWIEHW</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="105" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LFP7</accession>
    <name>Q1LFP7_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Response regulator, OmpR-family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Rmet_4162</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="6">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="6">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11029.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518651.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4162"/>
    <dbReference type="EnsemblBacteria" id="ABF11029">
        <property type="protein sequence ID" value="ABF11029"/>
        <property type="gene ID" value="Rmet_4162"/>
    </dbReference>
    <dbReference type="GeneID" id="60823257"/>
    <dbReference type="KEGG" id="rme:Rmet_4162"/>
    <dbReference type="eggNOG" id="COG0745">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_30_0_4"/>
    <dbReference type="OMA" id="RTMDTHV"/>
    <dbReference type="OrthoDB" id="817710at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00383">
        <property type="entry name" value="trans_reg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001867">
        <property type="entry name" value="OmpR/PhoB-type_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039420">
        <property type="entry name" value="WalR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48111">
        <property type="entry name" value="PTHR48111"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00486">
        <property type="entry name" value="Trans_reg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00862">
        <property type="entry name" value="Trans_reg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51755">
        <property type="entry name" value="OMPR_PHOB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="5" id="KW-0614">Plasmid</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Response regulatory" evidence="3">
        <location>
            <begin position="2"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="domain" description="OmpR/PhoB-type" evidence="4">
        <location>
            <begin position="128"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="OmpR/PhoB-type" evidence="2">
        <location>
            <begin position="128"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="1">
        <location>
            <position position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABF11029.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="232" mass="26130" checksum="35707574F35260A9" modified="2006-05-30" version="1">MRIASVEDDTSQAEQICQLLDEAGFECSSFATGSAFLRALRDQAFDLVVMDWQLPDVSGYELVSWVRQHSGRLPPILFLTSRIEESDIVAGLSVGADDYLAKPIRPGEFVARVRALLRRAYPKTLHEDDLIRQGAYIVDARRRVIALDGQNVDLSPREFDLALFLFRNIGRLLARDVIEQAVWGRAMDAASRTLDTHMSRLRIKLALRPENGVRLASVYSHGYRFEATAAHE</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LFT6</accession>
    <name>Q1LFT6_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Heavy metal cation tricomponent efflux pump HmyA (CzcA-like)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">hmyA</name>
        <name evidence="6" type="ordered locus">Rmet_4123</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="7">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="7">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the resistance-nodulation-cell division (RND) (TC 2.A.6) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10990.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4123"/>
    <dbReference type="EnsemblBacteria" id="ABF10990">
        <property type="protein sequence ID" value="ABF10990"/>
        <property type="gene ID" value="Rmet_4123"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4123"/>
    <dbReference type="eggNOG" id="COG3696">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002755_1_2_4"/>
    <dbReference type="OMA" id="IVMMMRG"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008324">
        <property type="term" value="F:cation transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2090.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027463">
        <property type="entry name" value="AcrB_DN_DC_subdom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001036">
        <property type="entry name" value="Acrflvin-R"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004763">
        <property type="entry name" value="CzcA/CusA/SilA/NccA/HelA/CnrA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32063">
        <property type="entry name" value="PTHR32063"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00873">
        <property type="entry name" value="ACR_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00702">
        <property type="entry name" value="ACRIFLAVINRP"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82714">
        <property type="entry name" value="SSF82714"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00914">
        <property type="entry name" value="2A0601"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0614">Plasmid</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5012565208" evidence="5">
        <location>
            <begin position="16"/>
            <end position="1023"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="326"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="356"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="382"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="423"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="459"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="523"/>
            <end position="545"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="858"/>
            <end position="875"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="882"/>
            <end position="902"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="908"/>
            <end position="932"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="953"/>
            <end position="973"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="985"/>
            <end position="1011"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="591"/>
            <end position="620"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABF10990.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="1023" mass="111443" checksum="0F15A25584197ABF" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-10-07" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LG34</accession>
    <name>Q1LG34_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_4024</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10892.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4024"/>
    <dbReference type="EnsemblBacteria" id="ABF10892">
        <property type="protein sequence ID" value="ABF10892"/>
        <property type="gene ID" value="Rmet_4024"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4024"/>
    <dbReference type="HOGENOM" id="CLU_2156269_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF10892.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="111" mass="12488" checksum="2A313A0E11AB9ECD" modified="2006-05-30" version="1">MWGGHEQRDCEALVIACRRSLADRILRPVSGAPACSARREGAIVKQNGAACRTRPGVDEKADRRTIRAQSAHDRAMVVDPTIRSHQYCAQGYRRTMRRPMLKLCDARVPYP</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LG51</accession>
    <name>Q1LG51_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Cupin_3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_4007</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10875.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4007"/>
    <dbReference type="EnsemblBacteria" id="ABF10875">
        <property type="protein sequence ID" value="ABF10875"/>
        <property type="gene ID" value="Rmet_4007"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4007"/>
    <dbReference type="eggNOG" id="COG3450">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_147448_1_1_4"/>
    <dbReference type="OMA" id="KGTWEVL"/>
    <dbReference type="OrthoDB" id="1928636at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011051">
        <property type="entry name" value="RmlC_Cupin_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008579">
        <property type="entry name" value="UGlyAH_Cupin_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05899">
        <property type="entry name" value="Cupin_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51182">
        <property type="entry name" value="SSF51182"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cupin_3" evidence="2">
        <location>
            <begin position="48"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF05899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF10875.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="128" mass="14082" checksum="A96DBE7212239468" modified="2006-05-30" version="1">MNSTTQPSLPTLTDFLGRPAPSHDNPRPDRLVKGNPDRTTWTHYSATQGDFDCGIWACEPGAWRIAFPAGKEEFFHVISGRLRITSLAGEAREFGPGDACVIPAGFEGVFEVLEPVRKHFVVIDRNAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LGE2</accession>
    <name>Q1LGE2_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative acetyl-CoA synthetase</fullName>
            <ecNumber evidence="3">6.2.1.13</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_3916</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EC" id="6.2.1.13" evidence="3"/>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10784.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518431.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3916"/>
    <dbReference type="EnsemblBacteria" id="ABF10784">
        <property type="protein sequence ID" value="ABF10784"/>
        <property type="gene ID" value="Rmet_3916"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3916"/>
    <dbReference type="eggNOG" id="COG1042">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007415_3_1_4"/>
    <dbReference type="OMA" id="YMEGCRD"/>
    <dbReference type="OrthoDB" id="445933at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043758">
        <property type="term" value="F:acetate-CoA ligase (ADP-forming) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.261">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011761">
        <property type="entry name" value="ATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013815">
        <property type="entry name" value="ATP_grasp_subdomain_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003781">
        <property type="entry name" value="CoA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032875">
        <property type="entry name" value="Succ_CoA_lig_flav_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016102">
        <property type="entry name" value="Succinyl-CoA_synth-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13380">
        <property type="entry name" value="CoA_binding_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13607">
        <property type="entry name" value="Succ_CoA_lig"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00881">
        <property type="entry name" value="CoA_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52210">
        <property type="entry name" value="SSF52210"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50975">
        <property type="entry name" value="ATP_GRASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0436">Ligase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ATP-grasp" evidence="2">
        <location>
            <begin position="500"/>
            <end position="536"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF10784.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="703" mass="72183" checksum="196C5B090EC63DAD" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LGJ7</accession>
    <name>Q1LGJ7_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Putative intramembrane serine protease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Rmet_3861</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="8">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="8">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10729.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3861"/>
    <dbReference type="EnsemblBacteria" id="ABF10729">
        <property type="protein sequence ID" value="ABF10729"/>
        <property type="gene ID" value="Rmet_3861"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3861"/>
    <dbReference type="eggNOG" id="COG0705">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_507940_0_0_4"/>
    <dbReference type="OMA" id="DHRQFFA"/>
    <dbReference type="OrthoDB" id="1565071at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
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    <keyword evidence="7" id="KW-0614">Plasmid</keyword>
    <keyword evidence="7" id="KW-0645">Protease</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
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        </location>
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        <location>
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            <end position="215"/>
        </location>
    </feature>
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        <location>
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            <end position="267"/>
        </location>
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        </location>
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            <end position="382"/>
        </location>
    </feature>
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            <begin position="394"/>
            <end position="413"/>
        </location>
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        <location>
            <begin position="237"/>
            <end position="378"/>
        </location>
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        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF01694"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="ABF10729.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="554" mass="60150" checksum="0E9DC2ABDD2E6F47" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LGQ1</accession>
    <name>Q1LGQ1_CUPMC</name>
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        <submittedName>
            <fullName evidence="1">Acetyl-CoA acetyltransferase</fullName>
            <ecNumber evidence="1">2.3.1.9</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_3805</name>
    </gene>
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        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
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            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
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        </lineage>
    </organism>
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        <name>megaplasmid CH34</name>
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    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
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            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
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    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
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    <dbReference type="EnsemblBacteria" id="ABF10675">
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        <property type="gene ID" value="Rmet_3805"/>
    </dbReference>
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    <dbReference type="KEGG" id="rme:Rmet_3805"/>
    <dbReference type="eggNOG" id="COG0183">
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    </dbReference>
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    <dbReference type="OrthoDB" id="647502at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000429">
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    </dbReference>
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        <property type="entry name" value="SSF53901"/>
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    </dbReference>
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    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF10675.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="388" mass="41022" checksum="463F00FB9C82DEA4" modified="2006-05-30" version="1">MTINSKAYIVGAYEHPTRKAPDKTVAQLHAESAKGALEDAGLSLADVDGYFCAGDAPGLGMVNMVDYLGLNVRHVDSTEMGGSSYIAHVSHAAQAIAAGKCNVALITLAGRPRSEGSSGTQARNWGANLPDQPFEAPFNPVTVNLYAMVAQRHMYEFGTTPEQLAWVKVAASHHAQYNPNAMLREVVTVEDVVNSPMISDPLHRLDCCVVSDGGGALIVARPEIAAKLNRPKIKILGAGEHVKGLLGGQVDLSWSAARFSGAAAFEEARVTPADIKYASIYDSFTITVLMQLEDLGFCKKGEGGKFVMDGNLISGVGKLPFNTDGGGLCNNHPANRGGITKVIEAVRQLRGEAHPAVQVKNCDLALAQGTGGYMGSRHGSATLILERE</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="102" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LH04</accession>
    <name>Q1LH04_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Flagellar biosynthesis protein FlhF</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Flagella-associated GTP-binding protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="primary">flhF</name>
        <name evidence="12" type="ordered locus">Rmet_3700</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="13">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="13">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Necessary for flagellar biosynthesis. May be involved in translocation of the flagellum.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the GTP-binding SRP family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10572.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3700"/>
    <dbReference type="EnsemblBacteria" id="ABF10572">
        <property type="protein sequence ID" value="ABF10572"/>
        <property type="gene ID" value="Rmet_3700"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3700"/>
    <dbReference type="eggNOG" id="COG1419">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009301_11_0_4"/>
    <dbReference type="OMA" id="QIYGRLM"/>
    <dbReference type="OrthoDB" id="1356333at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044781">
        <property type="term" value="P:bacterial-type flagellum organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006614">
        <property type="term" value="P:SRP-dependent cotranslational protein targeting to membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020006">
        <property type="entry name" value="FlhF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000897">
        <property type="entry name" value="SRP54_GTPase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43134:SF3">
        <property type="entry name" value="PTHR43134:SF3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00448">
        <property type="entry name" value="SRP54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00962">
        <property type="entry name" value="SRP54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03499">
        <property type="entry name" value="FlhF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1005">Bacterial flagellum biogenesis</keyword>
    <keyword evidence="7" id="KW-1006">Bacterial flagellum protein export</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="12" id="KW-0966">Cell projection</keyword>
    <keyword evidence="12" id="KW-0969">Cilium</keyword>
    <keyword evidence="12" id="KW-0282">Flagellum</keyword>
    <keyword evidence="6" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-0614">Plasmid</keyword>
    <keyword evidence="7" id="KW-0653">Protein transport</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0813">Transport</keyword>
    <feature type="domain" description="AAA" evidence="10">
        <location>
            <begin position="191"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="domain" description="SRP54" evidence="11">
        <location>
            <begin position="192"/>
            <end position="390"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008531"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022795"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00025337"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00030866"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ABF10572.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="825" mass="87546" checksum="E4B0142E846C896C" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="51" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LH76</accession>
    <name>Q1LH76_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_3628</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10500.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="gene ID" value="Rmet_3628"/>
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        <property type="component" value="Plasmid megaplasmid CH34"/>
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    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="60" mass="6921" checksum="86837155ACD3B793" modified="2006-05-30" version="1">MGMTIMQYPDASYFDRPVGEQLLSHFARRDAVRHTTPLLPFRFADVLALFRGTNAHRKTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="72" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LHF8</accession>
    <name>Q1LHF8_CUPMC</name>
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        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
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                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
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                <person name="Medigue C."/>
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                <person name="McCorkle S."/>
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            <dbReference type="PubMed" id="20463976"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="SSF103473"/>
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    </dbReference>
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    </dbReference>
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    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
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        <location>
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        </location>
    </feature>
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            <end position="100"/>
        </location>
    </feature>
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        </location>
    </feature>
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    </feature>
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        <location>
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            <end position="185"/>
        </location>
    </feature>
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            <end position="242"/>
        </location>
    </feature>
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    </feature>
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    </feature>
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        <location>
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        </location>
    </feature>
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        <location>
            <begin position="1"/>
            <end position="398"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="ABF10418.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="398" mass="41361" checksum="8FE82EC813EE07E7" modified="2006-05-30" version="1">MTSATAQRADAPAAWITPLLATACGMIVANLYYAQPLVGPIARALELSPEIAGLIVTLIQIGYCVGLLLLVPLGDIVENRKLVLALIAGCAVALVAAALATHASVFLIAGCAIGLCSVAVQVLVPFAAHLAPEHARGRAVGNVTSGLLMGIMLARPVSSLVADLFGWHTIFAASAVAMVLLGVVLSRKLPQRQPAPGVNYVEMLGSMGHLLRTQPVLRRRAMYQASMFGVFSLFWTTTPLYLAGPAFHMSQTGIAVFALVGVAGAIAAPMAGRYADRGHSRQMTAIALALGAGSFLLSRIGVEGSLMSLAALTVAAVVLDFAVSANLVIGQRAIFSLSAEHRSRLNGLYMAIFFVGGAIGSSVGAWAYARAGWPLASWIGFALPAIALLYFRTEPAST</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LHL8</accession>
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            <fullName evidence="6">Sodium/hydrogen exchanger family protein</fullName>
        </submittedName>
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    </gene>
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        <lineage>
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        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="ABF10358.1"/>
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    </dbReference>
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    <dbReference type="EnsemblBacteria" id="ABF10358">
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        <property type="gene ID" value="Rmet_3486"/>
    </dbReference>
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        <property type="taxonomic scope" value="Bacteria"/>
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    <dbReference type="OMA" id="FRHNPMV"/>
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        <property type="component" value="Chromosome"/>
    </dbReference>
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        <property type="term" value="C:integral component of membrane"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006812">
        <property type="term" value="P:cation transport"/>
        <property type="evidence" value="ECO:0007669"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006153">
        <property type="entry name" value="Cation/H_exchanger"/>
    </dbReference>
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        <property type="entry name" value="Na+/solute_symporter_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00999">
        <property type="entry name" value="Na_H_Exchanger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 4" id="KW-0472">Membrane</keyword>
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    <keyword evidence="1 4" id="KW-0812">Transmembrane</keyword>
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        <location>
            <begin position="20"/>
            <end position="38"/>
        </location>
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        </location>
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        </location>
    </feature>
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        </location>
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        <location>
            <begin position="24"/>
            <end position="363"/>
        </location>
    </feature>
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        <source>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="406" mass="42958" checksum="398E883054EA4E45" modified="2006-05-30" version="1">MSAMNGLSQLFPSLPLAPSGLFWVGLALVGAGLAGELCRRWLGFPRIVGYAAAGLFAGTLGRSIVDENMIAQTRILIDMALALALFELGHRLSFSWLRANRWLLFTSGAESLLTWGLVSTVLQWLGASPAVAILAGGIAVSTSPTIVLQLKNELRAEGQVTERLMAMAALNSIYAAVIVQVATGWLHSEYGNIGAALLHPLYLLTGSCLLAWVVGKVGHAIYARMSADDHYSFLVLVGLVLFALALTRVLKLSMPLSLMLAGVVFKHQDRQPHVWPTHFGSAGSLLIIVMIVSLGLPLTAHDWAVGGMLAIALVVMRHVAKLVGVISLGSFSGLSLRQCTALGLALAPMSGLAYLLMNDVALLYPGTGSPLASIILCALAIEQLLGPVIAAWALHYVSEARDNGGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LHT5</accession>
    <name>Q1LHT5_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Sulfur oxidation protein (SoxX)</fullName>
        </submittedName>
    </protein>
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    </gene>
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    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
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                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
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                <person name="Medigue C."/>
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            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="ABF10291.1"/>
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        <property type="nucleotide sequence ID" value="NC_007973.1"/>
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    <dbReference type="EnsemblBacteria" id="ABF10291">
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    </dbReference>
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    </dbReference>
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    <dbReference type="OrthoDB" id="1853490at2"/>
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        <property type="component" value="Chromosome"/>
    </dbReference>
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        <property type="term" value="F:electron transfer activity"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="F:heme binding"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Cyt_c-like_dom"/>
    </dbReference>
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        <property type="entry name" value="Cyt_c-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030999">
        <property type="entry name" value="Thiosulf_SoxX"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016823">
        <property type="entry name" value="Thiosulf_SoxX_II"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF024608">
        <property type="entry name" value="UCP024608"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46626">
        <property type="entry name" value="SSF46626"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR04485">
        <property type="entry name" value="thiosulf_SoxX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51007">
        <property type="entry name" value="CYTC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 4" id="KW-0349">Heme</keyword>
    <keyword evidence="3 4" id="KW-0408">Iron</keyword>
    <keyword evidence="2 4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193179" evidence="5">
        <location>
            <begin position="25"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="domain" description="Cytochrome c" evidence="6">
        <location>
            <begin position="108"/>
            <end position="215"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABF10291.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="220" mass="23618" checksum="D232A5FF43B607EC" modified="2006-05-30" version="1">MNKLHTMALVAMVALSAAGTQALAQTAAKGTTKGATTKVTDADVRQMIESSFTSKGPATVEGVLNQDATQKACSQYPDRSKVPAAVAKKVEAAELKQVKYPADNNWLGDWKEGEKIAQNGRGMQFTDAVGGTNGGNCYACHQLTKAEISFGNIGPSLYQYGKLRGNSQEVVRYTWGKIWDSNAFSACSNMPRFGHKGILTEQQIRDVMALLLDPASPVNQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LHV7</accession>
    <name>Q1LHV7_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative acyltransferase with acyl-CoA N-acyltransferase domain</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">ypeA</name>
        <name evidence="2" type="ordered locus">Rmet_3397</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10269.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517832.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3397"/>
    <dbReference type="EnsemblBacteria" id="ABF10269">
        <property type="protein sequence ID" value="ABF10269"/>
        <property type="gene ID" value="Rmet_3397"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3397"/>
    <dbReference type="eggNOG" id="COG0456">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013985_34_1_4"/>
    <dbReference type="OMA" id="IAGFDGW"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008080">
        <property type="term" value="F:N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00583">
        <property type="entry name" value="Acetyltransf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="SSF55729"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="1">
        <location>
            <begin position="1"/>
            <end position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF10269.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="139" mass="15602" checksum="6411B5C0916A71A9" modified="2006-05-30" version="1">MQIRPYNPTDEADVIALWKACGLTRPWNDPHRDIARKMTEQPEMFLIGDVDGHVRASAMVGFDGHRGWVYYLAVDPAHQGHGYGRMLMAHAEALLVERGCPKINLLVREGNDAVLAFYNELGYTRDAAVSLGKRLIPDQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="59" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LHY4</accession>
    <name>Q1LHY4_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Putative multicomponent oxygenase/reductase subunit for phenylacetic acid degradation</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">paaB</name>
        <name evidence="1" type="ordered locus">Rmet_3370</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10242.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008647104.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3370"/>
    <dbReference type="EnsemblBacteria" id="ABF10242">
        <property type="protein sequence ID" value="ABF10242"/>
        <property type="gene ID" value="Rmet_3370"/>
    </dbReference>
    <dbReference type="GeneID" id="60822474"/>
    <dbReference type="KEGG" id="rme:Rmet_3370"/>
    <dbReference type="eggNOG" id="COG3460">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_141459_2_0_4"/>
    <dbReference type="OMA" id="PYRHPTF"/>
    <dbReference type="OrthoDB" id="2083684at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.520">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009359">
        <property type="entry name" value="PaaB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038693">
        <property type="entry name" value="PaaB_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06243">
        <property type="entry name" value="PaaB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF030200">
        <property type="entry name" value="PaaB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02157">
        <property type="entry name" value="PA_CoA_Oxy2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF10242.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="95" mass="10833" checksum="83A1782134F2B893" modified="2006-05-30" version="1">MTQKEWPLWEVFVRSKQGLEHKHCGSLHAADAQQALLMARDVYTRRQEGVSIWVVPSSAITASAPAEKGELFDPAADKIYRHPTFYQLPEEVNHM</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="61" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LI75</accession>
    <name>Q1LI75_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative transmembrane transcriptional regulator (Anti-sigma factor)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_3279</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10151.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517753.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3279"/>
    <dbReference type="EnsemblBacteria" id="ABF10151">
        <property type="protein sequence ID" value="ABF10151"/>
        <property type="gene ID" value="Rmet_3279"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3279"/>
    <dbReference type="eggNOG" id="COG5662">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_083880_0_0_4"/>
    <dbReference type="OMA" id="MAFYWID"/>
    <dbReference type="OrthoDB" id="1494577at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="86"/>
            <end position="109"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF10151.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="277" mass="29634" checksum="827BC367E31A3477" modified="2006-05-30" version="1">MVTPTPVGEADLHAYADGQLSGPRRAEVEAFLAADPEAARKVEAWRRQTAGLHASLNAVLKEAVPLDALPRGLRAGSGRTKAHRPLWPVALALAASVATFIIGGTSGWMARGRFDADLGGVPPLERFARDALASHVVYAPEVRHVVEVPASDEAHMVAWLSKRLGAPLQVPDLRAQGFRLIGGRLGVAEGGPMAILMYENDAGTRLSLQLRRMAQGTPDTAFRLERLAPAQGAQSTGPAAMAFYWVDHNLGFALAGPIDRERLLALATAVYQQYQRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="98" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LIF6</accession>
    <name>Q1LIF6_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Adenosylhomocysteine nucleosidase</fullName>
            <ecNumber evidence="2">3.2.2.9</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">mtnN</name>
        <name evidence="7" type="ordered locus">Rmet_3198</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-methionine biosynthesis via salvage pathway; S-methyl-5-thio-alpha-D-ribose 1-phosphate from S-methyl-5'-thioadenosine (hydrolase route): step 1/2.</text>
    </comment>
    <dbReference type="EC" id="3.2.2.9" evidence="2"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10070.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3198"/>
    <dbReference type="EnsemblBacteria" id="ABF10070">
        <property type="protein sequence ID" value="ABF10070"/>
        <property type="gene ID" value="Rmet_3198"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3198"/>
    <dbReference type="eggNOG" id="COG0775">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031248_2_0_4"/>
    <dbReference type="OMA" id="DQFVHSK"/>
    <dbReference type="UniPathway" id="UPA00904">
        <property type="reaction ID" value="UER00871"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008782">
        <property type="term" value="F:adenosylhomocysteine nucleosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008930">
        <property type="term" value="F:methylthioadenosine nucleosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019509">
        <property type="term" value="P:L-methionine salvage from methylthioadenosine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009164">
        <property type="term" value="P:nucleoside catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1580">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010049">
        <property type="entry name" value="MTA_SAH_Nsdase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000845">
        <property type="entry name" value="Nucleoside_phosphorylase_d"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035994">
        <property type="entry name" value="Nucleoside_phosphorylase_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01048">
        <property type="entry name" value="PNP_UDP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53167">
        <property type="entry name" value="SSF53167"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01704">
        <property type="entry name" value="MTA/SAH-Nsdase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="7" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="4 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0486">Methionine biosynthesis</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PNP_UDP_1" evidence="6">
        <location>
            <begin position="32"/>
            <end position="280"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004945"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABF10070.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="284" mass="30559" checksum="0F3DB04CED904502" modified="2006-05-30" version="1">MHGARTFRPRRHTGMLALSSFPAFSALPAMTLGILAALHDEVDGLIAAMRHEDARATKRTIGMRDYYSGTLHGQPVVLVLARIGKVAAAATTVTLIREFGVNEIVFSGLAGGVGPEVRVGDIVVADRTVQHDLDARPLFPRHEVPLLARTEFPADPGLTGALRQAAEDFLRQDLATEVPDGVLARFGVRDPRLHVGMIASGDQFIGSPAAVSGLREQLPALQAVEMEGAALAQICYEYGVPYALLRTISDRADDTAHVDFSSFLRDVASHYSGAILRRFLDAKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LII7</accession>
    <name>Q1LII7_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Nitric oxide reductase large subunit</fullName>
            <ecNumber evidence="2">1.7.2.5</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">norB</name>
        <name evidence="2" type="ordered locus">Rmet_3167</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EC" id="1.7.2.5" evidence="2"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10039.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3167"/>
    <dbReference type="EnsemblBacteria" id="ABF10039">
        <property type="protein sequence ID" value="ABF10039"/>
        <property type="gene ID" value="Rmet_3167"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3167"/>
    <dbReference type="eggNOG" id="COG3256">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021582_0_0_4"/>
    <dbReference type="OMA" id="VTRTWHT"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004129">
        <property type="term" value="F:cytochrome-c oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016966">
        <property type="term" value="F:nitric oxide reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009060">
        <property type="term" value="P:aerobic respiration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.210.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036927">
        <property type="entry name" value="Cyt_c_oxase-like_su1_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000883">
        <property type="entry name" value="Cyt_C_Oxase_1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10422">
        <property type="entry name" value="PTHR10422"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00115">
        <property type="entry name" value="COX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81442">
        <property type="entry name" value="SSF81442"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="230"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="285"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="335"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="369"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="414"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="447"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="488"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="518"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="548"/>
            <end position="567"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="588"/>
            <end position="611"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="631"/>
            <end position="653"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="674"/>
            <end position="693"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="725"/>
            <end position="743"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF10039.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="759" mass="84094" checksum="5C6D9E93E5774DAF" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LIN2</accession>
    <name>Q1LIN2_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Glutathione-dependent peroxiredoxin</fullName>
            <ecNumber evidence="3">1.11.1.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Rmet_3122</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Thiol-specific peroxidase that catalyzes the reduction of hydrogen peroxide and organic hydroperoxides to water and alcohols, respectively. Plays a role in cell protection against oxidative stress by detoxifying peroxides.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>a hydroperoxide + 2 glutathione = an alcohol + glutathione disulfide + H2O</text>
            <dbReference type="Rhea" id="RHEA:62632"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30879"/>
            <dbReference type="ChEBI" id="CHEBI:35924"/>
            <dbReference type="ChEBI" id="CHEBI:57925"/>
            <dbReference type="ChEBI" id="CHEBI:58297"/>
            <dbReference type="EC" id="1.11.1.27"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the peroxiredoxin family. Prx5 subfamily.</text>
    </comment>
    <dbReference type="EC" id="1.11.1.27" evidence="3"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09994.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517616.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3122"/>
    <dbReference type="EnsemblBacteria" id="ABF09994">
        <property type="protein sequence ID" value="ABF09994"/>
        <property type="gene ID" value="Rmet_3122"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3122"/>
    <dbReference type="eggNOG" id="COG0678">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_072440_1_2_4"/>
    <dbReference type="OMA" id="GYINHPK"/>
    <dbReference type="OrthoDB" id="892697at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004601">
        <property type="term" value="F:peroxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03013">
        <property type="entry name" value="PRX5_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037944">
        <property type="entry name" value="PRX5-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013740">
        <property type="entry name" value="Redoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10430">
        <property type="entry name" value="PTHR10430"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08534">
        <property type="entry name" value="Redoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="SSF52833"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0049">Antioxidant</keyword>
    <keyword evidence="2 3 5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="1 3 5" id="KW-0575">Peroxidase</keyword>
    <keyword evidence="3" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thioredoxin" evidence="4">
        <location>
            <begin position="2"/>
            <end position="168"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU366011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABF09994.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="168" mass="18309" checksum="C2E3F01C980B72C2" modified="2006-05-30" version="1">MIAIGQRVPDATLYEFFETESEGCVLGPNAFSVAELVKGRKIVVFGLPGAFTPTCSAKHVPSYLKEYDALRAAGVDEIWCHSVNDAFVMGAWGREQKATGKVRMMGDGAAQWARALGLDQDLSQRGLGVRAKRYAMVVDDGVVTHLFIEEPGEFKVSSAEWVLQALRA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-10-31" modified="2022-08-03" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LIU6</accession>
    <name>UBIC_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Probable chorismate pyruvate-lyase</fullName>
            <shortName evidence="1">CL</shortName>
            <shortName evidence="1">CPL</shortName>
            <ecNumber evidence="1">4.1.3.40</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">ubiC</name>
        <name type="ordered locus">Rmet_3058</name>
    </gene>
    <organism>
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Removes the pyruvyl group from chorismate, with concomitant aromatization of the ring, to provide 4-hydroxybenzoate (4HB) for the ubiquinone pathway.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>chorismate = 4-hydroxybenzoate + pyruvate</text>
            <dbReference type="Rhea" id="RHEA:16505"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:17879"/>
            <dbReference type="ChEBI" id="CHEBI:29748"/>
            <dbReference type="EC" id="4.1.3.40"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; ubiquinone biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the UbiC family.</text>
    </comment>
    <comment type="sequence caution" evidence="3">
        <conflict type="erroneous initiation">
            <sequence resource="EMBL-CDS" id="ABF09930" version="1"/>
        </conflict>
        <text>Extended N-terminus.</text>
    </comment>
    <dbReference type="EC" id="4.1.3.40" evidence="1"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09930.1"/>
        <property type="status" value="ALT_INIT"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_029307163.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q1LIU6"/>
    <dbReference type="SMR" id="Q1LIU6"/>
    <dbReference type="STRING" id="266264.Rmet_3058"/>
    <dbReference type="EnsemblBacteria" id="ABF09930">
        <property type="protein sequence ID" value="ABF09930"/>
        <property type="gene ID" value="Rmet_3058"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3058"/>
    <dbReference type="eggNOG" id="COG3161">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_096824_2_0_4"/>
    <dbReference type="OrthoDB" id="1274776at2"/>
    <dbReference type="UniPathway" id="UPA00232"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008813">
        <property type="term" value="F:chorismate lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042866">
        <property type="term" value="P:pyruvate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006744">
        <property type="term" value="P:ubiquinone biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1410.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01632">
        <property type="entry name" value="UbiC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007440">
        <property type="entry name" value="Chorismate--pyruvate_lyase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028978">
        <property type="entry name" value="Chorismate_lyase_/UTRA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38683">
        <property type="entry name" value="PTHR38683"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04345">
        <property type="entry name" value="Chor_lyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64288">
        <property type="entry name" value="SSF64288"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0456">Lyase</keyword>
    <keyword id="KW-0670">Pyruvate</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0831">Ubiquinone biosynthesis</keyword>
    <feature type="chain" id="PRO_0000255911" description="Probable chorismate pyruvate-lyase">
        <location>
            <begin position="1"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="183"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="196"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="74"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="112"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="173"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01632"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="214" mass="24061" checksum="F1EC498766F0D862" modified="2006-10-31" version="2">MQKLRAAWHAHLPYDASIPLNQRRWITGEGSLTARLMSASAAFRVRRLAQAPQLPLADEWRALGLIRPLPAITREVLLICDETPAVFAHTIVDPRYARRDWPFLRGLGNRPLGGALFVDPRVRRDPFQFARLTPCHPLRQALQRVLPALSSEPMLPARRSVFRRGGGAMLVTEVFLPDLLTRAAPENTGAGGTRLPRRIDTHHTPSKQEERPES</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LIU9</accession>
    <name>Q1LIU9_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Putative TonB-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Rmet_3055</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09927.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3055"/>
    <dbReference type="EnsemblBacteria" id="ABF09927">
        <property type="protein sequence ID" value="ABF09927"/>
        <property type="gene ID" value="Rmet_3055"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3055"/>
    <dbReference type="eggNOG" id="COG0810">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_052089_0_0_4"/>
    <dbReference type="OMA" id="NRDGSLY"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006260">
        <property type="entry name" value="TonB/TolA_C"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01352">
        <property type="entry name" value="tonB_Cterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="94"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="123"/>
            <end position="144"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABF09927.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="328" mass="36462" checksum="EFF388FEC3B1B832" modified="2006-05-30" version="1">MAGVSIDSCRRPPIPIVNAVLPAPRLPRHPREWWQSSSTLTKALAISVAVHALLLMVRVAAPEVFEIKRSDPQLDVVLVNSKSAVKPRNPTVLAQANLDGGGEHDAQRATTPLPAQTESKEGDLIKMTQRRVEQLEEEQRRLMTQSREAAPAVRSQPVKPGEQRSDQPTRGQDDRTSNDEMARLEAEIGRNLEHYAKRPKRMQLTATSAQGVDYAQYYDRLRRKIETRGTSDFPQRNGKPIYGQLILVINVNRQGKLGYNRDGYNVEAIDVAKSSGDPALDRQAVAIVRAAAPFGAFTAEMQAKQDILEVVSTFKFTRSGLDVRLQGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="104" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LJ89</accession>
    <name>Q1LJ89_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Poly(A) polymerase I</fullName>
            <shortName evidence="9">PAP I</shortName>
            <ecNumber evidence="9">2.7.7.19</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9 15" type="primary">pcnB</name>
        <name evidence="15" type="ordered locus">Rmet_2914</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Adds poly(A) tail to the 3' end of many RNAs, which usually targets these RNAs for decay. Plays a significant role in the global control of gene expression, through influencing the rate of transcript degradation, and in the general RNA quality control.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>ATP + RNA(n) = diphosphate + RNA(n)-3'-adenine ribonucleotide</text>
            <dbReference type="Rhea" id="RHEA:11332"/>
            <dbReference type="Rhea" id="RHEA-COMP:14527"/>
            <dbReference type="Rhea" id="RHEA-COMP:17347"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:140395"/>
            <dbReference type="ChEBI" id="CHEBI:173115"/>
            <dbReference type="EC" id="2.7.7.19"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="9 10">Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.19" evidence="9"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09787.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517460.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2914"/>
    <dbReference type="EnsemblBacteria" id="ABF09787">
        <property type="protein sequence ID" value="ABF09787"/>
        <property type="gene ID" value="Rmet_2914"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2914"/>
    <dbReference type="eggNOG" id="COG0617">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015961_0_0_4"/>
    <dbReference type="OMA" id="FMAKLDM"/>
    <dbReference type="OrthoDB" id="2018439at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004652">
        <property type="term" value="F:polynucleotide adenylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006378">
        <property type="term" value="P:mRNA polyadenylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05398">
        <property type="entry name" value="NT_ClassII-CCAase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.460.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00957">
        <property type="entry name" value="PolyA_pol"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043519">
        <property type="entry name" value="NT_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002646">
        <property type="entry name" value="PolA_pol_head_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010206">
        <property type="entry name" value="PolA_pol_I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025866">
        <property type="entry name" value="PolyA_pol_arg_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032828">
        <property type="entry name" value="PolyA_RNA-bd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01743">
        <property type="entry name" value="PolyA_pol"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12626">
        <property type="entry name" value="PolyA_pol_arg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12627">
        <property type="entry name" value="PolyA_pol_RNAbd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81301">
        <property type="entry name" value="SSF81301"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01942">
        <property type="entry name" value="pcnB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0460">Magnesium</keyword>
    <keyword evidence="1 9" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="8" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3 15" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9 10" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="7 9" id="KW-0804">Transcription</keyword>
    <keyword evidence="2 9 10" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PolyA_pol" evidence="12">
        <location>
            <begin position="59"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="domain" description="PolyA_pol_RNAbd" evidence="14">
        <location>
            <begin position="235"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="domain" description="PolyA_pol_arg_C" evidence="13">
        <location>
            <begin position="348"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="451"/>
            <end position="529"/>
        </location>
    </feature>
    <feature type="active site" evidence="9">
        <location>
            <position position="77"/>
        </location>
    </feature>
    <feature type="active site" evidence="9">
        <location>
            <position position="79"/>
        </location>
    </feature>
    <feature type="active site" evidence="9">
        <location>
            <position position="177"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00957"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU003953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF01743"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF12626"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF12627"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="ABF09787.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="529" mass="59271" checksum="8B0123BEB9094ADA" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LJY0</accession>
    <name>Q1LJY0_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Cell division coordinator CpoB</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">cpoB</name>
        <name evidence="4" type="ordered locus">Rmet_2673</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Mediates coordination of peptidoglycan synthesis and outer membrane constriction during cell division.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Periplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the CpoB family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09546.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008642274.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2673"/>
    <dbReference type="EnsemblBacteria" id="ABF09546">
        <property type="protein sequence ID" value="ABF09546"/>
        <property type="gene ID" value="Rmet_2673"/>
    </dbReference>
    <dbReference type="GeneID" id="60820889"/>
    <dbReference type="KEGG" id="rme:Rmet_2673"/>
    <dbReference type="eggNOG" id="COG1729">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_044315_1_0_4"/>
    <dbReference type="OMA" id="IDMLTQN"/>
    <dbReference type="OrthoDB" id="1099900at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030288">
        <property type="term" value="C:outer membrane-bounded periplasmic space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043093">
        <property type="term" value="P:FtsZ-dependent cytokinesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070206">
        <property type="term" value="P:protein trimerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02066">
        <property type="entry name" value="CpoB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039565">
        <property type="entry name" value="BamD-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034706">
        <property type="entry name" value="CpoB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014162">
        <property type="entry name" value="CpoB_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032519">
        <property type="entry name" value="YbgF_tri"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16331">
        <property type="entry name" value="TolA_bind_tri"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13525">
        <property type="entry name" value="YfiO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="SSF48452"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02795">
        <property type="entry name" value="tol_pal_ybgF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="1" id="KW-0132">Cell division</keyword>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0574">Periplasm</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009992470" description="Cell division coordinator CpoB" evidence="1">
        <location>
            <begin position="28"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="domain" description="TolA_bind_tri" evidence="3">
        <location>
            <begin position="56"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="domain" description="YfiO" evidence="2">
        <location>
            <begin position="132"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="57"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF16331"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF09546.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="252" mass="27756" checksum="D0873A1B6428FC56" modified="2006-05-30" version="1" precursor="true">MNARVSTLLWLAAVSGGGLLAAIPAHAGVFDDDEARRAVIQIRDQFNGFQATASQRIDQNSRTLLDAQNQLEGLRQEVARLRGQNEVLQNTVDTLQKQQKDYYADLDARLKKFEPQQVTVEGREGIAQPTEKPEYDAALKQFQSGDFKGAGNAFSAFAKKYPQSPYLPLAQFWLGNSLYAQRDYKGSTYVLDTMVKNFPTHPKAPDAMIAIANNQFESGQKAAAKKTLEAVVAKYPGTEGAQAASNRLKTLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LK14</accession>
    <name>Q1LK14_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Long-chain-fatty-acid-CoA ligase (Acyl-CoA synthetase (AMP-forming)/AMP-acid ligase II)</fullName>
            <ecNumber evidence="3">6.2.1.3</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">fadD</name>
        <name evidence="3" type="ordered locus">Rmet_2635</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EC" id="6.2.1.3" evidence="3"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09512.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517211.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2635"/>
    <dbReference type="EnsemblBacteria" id="ABF09512">
        <property type="protein sequence ID" value="ABF09512"/>
        <property type="gene ID" value="Rmet_2635"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2635"/>
    <dbReference type="eggNOG" id="COG0318">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000022_59_9_4"/>
    <dbReference type="OMA" id="THWTNIP"/>
    <dbReference type="OrthoDB" id="961884at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004467">
        <property type="term" value="F:long-chain fatty acid-CoA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025110">
        <property type="entry name" value="AMP-bd_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045851">
        <property type="entry name" value="AMP-bd_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020845">
        <property type="entry name" value="AMP-binding_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_Synth/Lig"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13193">
        <property type="entry name" value="AMP-binding_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00455">
        <property type="entry name" value="AMP_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0436">Ligase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AMP-binding" evidence="1">
        <location>
            <begin position="34"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="domain" description="AMP-binding_C" evidence="2">
        <location>
            <begin position="473"/>
            <end position="550"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF09512.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="566" mass="62562" checksum="ACA5A1A30A907295" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="102" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LKD3</accession>
    <name>Q1LKD3_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="13">ABC-type transporter, ATPase and permease component</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ordered locus">Rmet_2516</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09393.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517092.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2516"/>
    <dbReference type="EnsemblBacteria" id="ABF09393">
        <property type="protein sequence ID" value="ABF09393"/>
        <property type="gene ID" value="Rmet_2516"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2516"/>
    <dbReference type="eggNOG" id="COG1132">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_84_5_4"/>
    <dbReference type="OMA" id="ETLMQGK"/>
    <dbReference type="OrthoDB" id="643917at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1560.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011527">
        <property type="entry name" value="ABC1_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036640">
        <property type="entry name" value="ABC1_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039421">
        <property type="entry name" value="Type_1_exporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24221">
        <property type="entry name" value="PTHR24221"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00664">
        <property type="entry name" value="ABC_membrane"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90123">
        <property type="entry name" value="SSF90123"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50929">
        <property type="entry name" value="ABC_TM1F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="1" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-1278">Translocase</keyword>
    <keyword evidence="3 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="39"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="84"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="157"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="185"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="268"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="12">
        <location>
            <begin position="41"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="11">
        <location>
            <begin position="362"/>
            <end position="601"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="395"/>
            <end position="402"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ABF09393.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="610" mass="67359" checksum="E6808B8226CDFE1F" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-12-12" modified="2022-05-25" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LKN9</accession>
    <name>UVRC_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">UvrABC system protein C</fullName>
            <shortName evidence="1">Protein UvrC</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Excinuclease ABC subunit C</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">uvrC</name>
        <name type="ordered locus">Rmet_2410</name>
    </gene>
    <organism>
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">The UvrABC repair system catalyzes the recognition and processing of DNA lesions. UvrC both incises the 5' and 3' sides of the lesion. The N-terminal half is responsible for the 3' incision and the C-terminal half is responsible for the 5' incision.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Interacts with UvrB in an incision complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the UvrC family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09287.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517006.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q1LKN9"/>
    <dbReference type="SMR" id="Q1LKN9"/>
    <dbReference type="STRING" id="266264.Rmet_2410"/>
    <dbReference type="PRIDE" id="Q1LKN9"/>
    <dbReference type="EnsemblBacteria" id="ABF09287">
        <property type="protein sequence ID" value="ABF09287"/>
        <property type="gene ID" value="Rmet_2410"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2410"/>
    <dbReference type="eggNOG" id="COG0322">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014841_3_0_4"/>
    <dbReference type="OMA" id="HIECFDN"/>
    <dbReference type="OrthoDB" id="1036075at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009380">
        <property type="term" value="C:excinuclease repair complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009381">
        <property type="term" value="F:excinuclease ABC activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006289">
        <property type="term" value="P:nucleotide-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009432">
        <property type="term" value="P:SOS response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.340">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1440.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00203">
        <property type="entry name" value="UvrC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000305">
        <property type="entry name" value="GIY-YIG_endonuc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035901">
        <property type="entry name" value="GIY-YIG_endonuc_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010994">
        <property type="entry name" value="RuvA_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001943">
        <property type="entry name" value="UVR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036876">
        <property type="entry name" value="UVR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004791">
        <property type="entry name" value="UvrC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001162">
        <property type="entry name" value="UvrC_RNase_H_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038476">
        <property type="entry name" value="UvrC_RNase_H_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01541">
        <property type="entry name" value="GIY-YIG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02151">
        <property type="entry name" value="UVR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08459">
        <property type="entry name" value="UvrC_HhH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00465">
        <property type="entry name" value="GIYc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46600">
        <property type="entry name" value="SSF46600"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47781">
        <property type="entry name" value="SSF47781"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82771">
        <property type="entry name" value="SSF82771"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00194">
        <property type="entry name" value="uvrC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50164">
        <property type="entry name" value="GIY_YIG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50151">
        <property type="entry name" value="UVR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50165">
        <property type="entry name" value="UVRC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0227">DNA damage</keyword>
    <keyword id="KW-0228">DNA excision</keyword>
    <keyword id="KW-0234">DNA repair</keyword>
    <keyword id="KW-0267">Excision nuclease</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0742">SOS response</keyword>
    <feature type="chain" id="PRO_0000264929" description="UvrABC system protein C">
        <location>
            <begin position="1"/>
            <end position="673"/>
        </location>
    </feature>
    <feature type="domain" description="GIY-YIG" evidence="1">
        <location>
            <begin position="39"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="domain" description="UVR" evidence="1">
        <location>
            <begin position="226"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="673" mass="74070" checksum="F686AC12A2FE3466" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="64" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LKV0</accession>
    <name>Q1LKV0_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_2349</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09226.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004350603.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2349"/>
    <dbReference type="EnsemblBacteria" id="ABF09226">
        <property type="protein sequence ID" value="ABF09226"/>
        <property type="gene ID" value="Rmet_2349"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2349"/>
    <dbReference type="eggNOG" id="ENOG502Z7PS">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_100592_0_0_4"/>
    <dbReference type="OMA" id="MRAPDNL"/>
    <dbReference type="OrthoDB" id="1234627at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021548">
        <property type="entry name" value="DUF2895"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11444">
        <property type="entry name" value="DUF2895"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03746">
        <property type="entry name" value="conj_TIGR03746"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="20"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="205"/>
            <end position="230"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF09226.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="230" mass="25835" checksum="F8BCB685E8543A5E" modified="2006-05-30" version="1">MSRFKNEIAHLQAHIKTLRLGAGALVIVALVMGGGWWSAPRDLTIHVPPDLRSGSTRKWWEVPPESVYAFTFYVFQTLNRWPTNGEEDYSRNLHTLSPYLTPSCQAFLRADYDYRRSTGELRQRVRGIYEIPGRGYGDDPTARVRTVSDRDWVVTLDITADEYYGAEQVKRALVRYPIKVTRVDVDPARNPFGLALDCYEGAPQRISAPEPARPASSGLSPQAPQGGNTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-10-07" version="52" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LKX9</accession>
    <name>Q1LKX9_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_2320</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09197.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2320"/>
    <dbReference type="EnsemblBacteria" id="ABF09197">
        <property type="protein sequence ID" value="ABF09197"/>
        <property type="gene ID" value="Rmet_2320"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2320"/>
    <dbReference type="eggNOG" id="ENOG502Z9W9">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_056377_0_0_4"/>
    <dbReference type="OMA" id="EYRYINT"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021960">
        <property type="entry name" value="DUF3577"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12101">
        <property type="entry name" value="DUF3577"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="284"/>
            <end position="332"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF09197.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="332" mass="36410" checksum="A829B974517AA55A" modified="2006-05-30" version="1">MLSPYQPTAGSRTFPACVGRCVSASSQWRFTMNTTSNEKSYFDLHTSGIGYIQRVREVPVRGGRRAQPFLACTIAALVGPARDPSYRYFDVKVSGAEAKKLVQRYIGVDDPKQRPLVRFRLGDLWGDVYIRDKGEQKGQAAASLKARLLKAELIDRAELASIEQHELITRGIGYLNRVKDVTPKAGDSFLSCTVAALAGPVDEPEYRYFDTIVATPEAEHLVRRCVQAVEGDRKVLIAFRLNDMKIDPYIRTKGEHAGEPAASLESTLVHIGLIKIDGTQVYPTSQAQAEAPPAEDASASEADDAIDTATEPAEREPEAQVQEQEPALAASF</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="60" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LL17</accession>
    <name>Q1LL17_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Hemin uptake protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">hemP</name>
        <name evidence="1" type="ordered locus">Rmet_2280</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09159.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516984.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2280"/>
    <dbReference type="EnsemblBacteria" id="ABF09159">
        <property type="protein sequence ID" value="ABF09159"/>
        <property type="gene ID" value="Rmet_2280"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2280"/>
    <dbReference type="eggNOG" id="COG4256">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2329017_0_0_4"/>
    <dbReference type="OMA" id="IMHGAST"/>
    <dbReference type="OrthoDB" id="1966462at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019600">
        <property type="entry name" value="Hemin_uptake_protein_HemP"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10636">
        <property type="entry name" value="hemP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF09159.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="108" mass="11834" checksum="1820333C7E4E6B90" modified="2006-05-30" version="1">MSTLSLPMSQPREVTRRRLSLRRVEVVQQDRRETSTASSAIESVKSAVAALPARLEALMRQDAPAVGSTAQPVPLEAIMHGATTLPILHNGNVYTLRVTRYGKLILTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LL24</accession>
    <name>Q1LL24_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Fumarate hydratase class I</fullName>
            <ecNumber evidence="10">4.2.1.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">fumA</name>
        <name evidence="13" type="ordered locus">Rmet_2273</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Catalyzes the reversible hydration of fumarate to (S)-malate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 10">
            <text>(S)-malate = fumarate + H2O</text>
            <dbReference type="Rhea" id="RHEA:12460"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15589"/>
            <dbReference type="ChEBI" id="CHEBI:29806"/>
            <dbReference type="EC" id="4.2.1.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 10">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="4 10">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the class-I fumarase family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.2" evidence="10"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09152.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2273"/>
    <dbReference type="EnsemblBacteria" id="ABF09152">
        <property type="protein sequence ID" value="ABF09152"/>
        <property type="gene ID" value="Rmet_2273"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2273"/>
    <dbReference type="eggNOG" id="COG1838">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG1951">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041245_2_0_4"/>
    <dbReference type="OMA" id="AGVLPMC"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004333">
        <property type="term" value="F:fumarate hydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006091">
        <property type="term" value="P:generation of precursor metabolites and energy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.130.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004646">
        <property type="entry name" value="Fe-S_hydro-lyase_TtdA-typ_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004647">
        <property type="entry name" value="Fe-S_hydro-lyase_TtdB-typ_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036660">
        <property type="entry name" value="Fe-S_hydroAse_TtdB_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011167">
        <property type="entry name" value="Fe_dep_fumarate_hydratase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05681">
        <property type="entry name" value="Fumerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05683">
        <property type="entry name" value="Fumerase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001394">
        <property type="entry name" value="Fe_dep_fumar_hy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117457">
        <property type="entry name" value="SSF117457"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00722">
        <property type="entry name" value="ttdA_fumA_fumB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00723">
        <property type="entry name" value="ttdB_fumA_fumB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 10" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="7 10" id="KW-0408">Iron</keyword>
    <keyword evidence="8 10" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="9 10 13" id="KW-0456">Lyase</keyword>
    <keyword evidence="6 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fumerase" evidence="11">
        <location>
            <begin position="25"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="domain" description="Fumerase_C" evidence="12">
        <location>
            <begin position="303"/>
            <end position="504"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008876"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRNR" id="PIRNR001394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF05681"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF05683"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ABF09152.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="521" mass="55777" checksum="2C2BA4D6DE7C8DAE" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LLT5</accession>
    <name>Q1LLT5_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_2012</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08891.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516726.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2012"/>
    <dbReference type="EnsemblBacteria" id="ABF08891">
        <property type="protein sequence ID" value="ABF08891"/>
        <property type="gene ID" value="Rmet_2012"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2012"/>
    <dbReference type="HOGENOM" id="CLU_2071165_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF08891.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="118" mass="12555" checksum="87DA60C21E27EF83" modified="2006-05-30" version="1">MDDDTLGAGLHLAWPTAEGYRHFMVAVLGADGKAADYVMVGASRPDLPALAQRYLSGAREFDDATRRDMERWLGQSIESAIAAALETCIRNLSTLTVGDISAKASRPRGDSKGGMGNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="61" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LM31</accession>
    <name>Q1LM31_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Isochorismatase hydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_1916</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08795.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1916"/>
    <dbReference type="EnsemblBacteria" id="ABF08795">
        <property type="protein sequence ID" value="ABF08795"/>
        <property type="gene ID" value="Rmet_1916"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1916"/>
    <dbReference type="eggNOG" id="COG1335">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_066901_1_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.850">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000868">
        <property type="entry name" value="Isochorismatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036380">
        <property type="entry name" value="Isochorismatase-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00857">
        <property type="entry name" value="Isochorismatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52499">
        <property type="entry name" value="SSF52499"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Isochorismatase" evidence="1">
        <location>
            <begin position="6"/>
            <end position="144"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF08795.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="204" mass="22639" checksum="F1FDD9755862F262" modified="2006-05-30" version="1">MAFGIQSIDRQTLKNNVVGLAKAAKIFNIPTTISTVESESFSGYTFPELLDVFPNAKTLERSSMNSWDDQKVRDALKAAGRKKIVAAGLWTEVCITTFALCAMQDAGYEFYVVADACGGNTREAHDYAMQRMIQAGVVPVTWQQVLLEWQRDWAHHDTYDAVMQLVKEHSGAYGMGVDYAYTMVHKAAQRTATPHESLAPVPAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LM73</accession>
    <name>Q1LM73_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">TRAP-type transporter, periplasmic component / DctP analogue</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_1874</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08753.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516600.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1874"/>
    <dbReference type="EnsemblBacteria" id="ABF08753">
        <property type="protein sequence ID" value="ABF08753"/>
        <property type="gene ID" value="Rmet_1874"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1874"/>
    <dbReference type="eggNOG" id="COG1638">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036176_2_3_4"/>
    <dbReference type="OMA" id="TQAWLPK"/>
    <dbReference type="OrthoDB" id="752834at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071702">
        <property type="term" value="P:organic substance transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018389">
        <property type="entry name" value="DctP_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038404">
        <property type="entry name" value="TRAP_DctP_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33376">
        <property type="entry name" value="PTHR33376"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03480">
        <property type="entry name" value="DctP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193612" evidence="2">
        <location>
            <begin position="27"/>
            <end position="327"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF08753.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="327" mass="35310" checksum="94CFAD654D15584C" modified="2006-05-30" version="1">MRVKVLAVAVTMAVTGVLLTATAARADTKWDLPTGYPATNLHTENLQQMANDVDKATGGKLKIVLHPNGSLLKANEIKRGVQTGQVQMGEILMSTLANENPVFGVDAVPFLATSYGDSYKLWQASRTVTDKILDRQGMKLLYAVAWPPQGIYANKPLNSAADMKGLKWRAYNPATSKIAELVSAQPVTIQAADLAQALATGTVNSFMSSGATGVDTKVWESVKYFYTVDAWLPKNMLVVSKKAFNGLDKPTQDALLKAVADAEKRGWQVSEQKTREYLATLSKNGMTVAPPSPQLKSDMQKVGEVMVADWAKSAGDDGKAILDAYKK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-01-15" modified="2022-08-03" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LM81</accession>
    <name>YCIB_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Inner membrane-spanning protein YciB</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">yciB</name>
        <name type="ordered locus">Rmet_1866</name>
    </gene>
    <organism>
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Plays a role in cell envelope biogenesis, maintenance of cell envelope integrity and membrane homeostasis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the YciB family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08745.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008650249.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q1LM81"/>
    <dbReference type="STRING" id="266264.Rmet_1866"/>
    <dbReference type="EnsemblBacteria" id="ABF08745">
        <property type="protein sequence ID" value="ABF08745"/>
        <property type="gene ID" value="Rmet_1866"/>
    </dbReference>
    <dbReference type="GeneID" id="60825641"/>
    <dbReference type="KEGG" id="rme:Rmet_1866"/>
    <dbReference type="eggNOG" id="COG2917">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_089554_2_0_4"/>
    <dbReference type="OMA" id="AWVNFKL"/>
    <dbReference type="OrthoDB" id="1886685at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005887">
        <property type="term" value="C:integral component of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00189">
        <property type="entry name" value="YciB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006008">
        <property type="entry name" value="YciB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36917">
        <property type="entry name" value="PTHR36917"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04279">
        <property type="entry name" value="IspA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00997">
        <property type="entry name" value="ispZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0997">Cell inner membrane</keyword>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_1000021048" description="Inner membrane-spanning protein YciB">
        <location>
            <begin position="1"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="3"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="49"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="76"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="121"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="149"/>
            <end position="169"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00189"/>
        </source>
    </evidence>
    <sequence length="179" mass="20408" checksum="1FB13F764F4DFCD2" modified="2006-05-30" version="1">MKFLFDLFPVILFFVAFKLFGIYPATAVAIGATVVQIAWVHFRHGKAEPMQWVSLAIIAVFGGATILLHNETFIKWKPTVLYWLFAVTLIGSVIGWRKNLIRAMMEKQVTLPEPMWGRLNVAWAGFFAVMGVLNLYVAYQFSTDTWVNFKLFGSMGLMLVFIVAQSIWLSRHIQETPSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="104" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LM82</accession>
    <name>Q1LM82_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Peptide methionine sulfoxide reductase MsrB</fullName>
            <ecNumber evidence="3">1.8.4.12</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">Peptide-methionine (R)-S-oxide reductase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="3 5" type="primary">msrB</name>
        <name evidence="5" type="ordered locus">Rmet_1865</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 3">
            <text>[thioredoxin]-disulfide + H2O + L-methionyl-[protein] = [thioredoxin]-dithiol + L-methionyl-(R)-S-oxide-[protein]</text>
            <dbReference type="Rhea" id="RHEA:24164"/>
            <dbReference type="Rhea" id="RHEA-COMP:10698"/>
            <dbReference type="Rhea" id="RHEA-COMP:10700"/>
            <dbReference type="Rhea" id="RHEA-COMP:12313"/>
            <dbReference type="Rhea" id="RHEA-COMP:12314"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16044"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:45764"/>
            <dbReference type="ChEBI" id="CHEBI:50058"/>
            <dbReference type="EC" id="1.8.4.12"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="3">Binds 1 zinc ion per subunit. The zinc ion is important for the structural integrity of the protein.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the MsrB Met sulfoxide reductase family.</text>
    </comment>
    <dbReference type="EC" id="1.8.4.12" evidence="3"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08744.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008650248.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1865"/>
    <dbReference type="EnsemblBacteria" id="ABF08744">
        <property type="protein sequence ID" value="ABF08744"/>
        <property type="gene ID" value="Rmet_1865"/>
    </dbReference>
    <dbReference type="GeneID" id="60825642"/>
    <dbReference type="KEGG" id="rme:Rmet_1865"/>
    <dbReference type="eggNOG" id="COG0229">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031040_8_5_4"/>
    <dbReference type="OMA" id="DEQWRAE"/>
    <dbReference type="OrthoDB" id="1650261at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033743">
        <property type="term" value="F:peptide-methionine (R)-S-oxide reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030091">
        <property type="term" value="P:protein repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006979">
        <property type="term" value="P:response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01400">
        <property type="entry name" value="MsrB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028427">
        <property type="entry name" value="Met_Sox_Rdtase_MsrB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002579">
        <property type="entry name" value="Met_Sox_Rdtase_MsrB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011057">
        <property type="entry name" value="Mss4-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10173">
        <property type="entry name" value="PTHR10173"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01641">
        <property type="entry name" value="SelR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51316">
        <property type="entry name" value="SSF51316"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00357">
        <property type="entry name" value="TIGR00357"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51790">
        <property type="entry name" value="MSRB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2 3 5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="MsrB" evidence="4">
        <location>
            <begin position="7"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="3">
        <location>
            <position position="118"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="46"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="49"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="95"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="98"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001795"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01400"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51790"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABF08744.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="135" mass="15286" checksum="68F7C80FF6E8DFD9" modified="2006-05-30" version="1">MTTTKTDAEWRSQLSDIEYRVTREAATERPFTGQYWDHWNQGIYHCVGCGTPLFESATKFDAGCGWPSYFRPINGEVIAEHTDHSHGMTRVEVRCKECGSHLGHVFEDGPAPTGLRYCINSAALKFDDRDPEDRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="56" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LMK1</accession>
    <name>Q1LMK1_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative transposase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_1746</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08625.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF08625">
        <property type="protein sequence ID" value="ABF08625"/>
        <property type="gene ID" value="Rmet_1746"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1746"/>
    <dbReference type="eggNOG" id="COG2801">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2603479_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="44"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="50"/>
            <end position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF08625.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="79" mass="8667" checksum="35AC31158F3A3393" modified="2006-05-30" version="1">MAEYPEGHVELWADAAPLPYTTYDRFPEVDQRAIVDNKRQGLALASTAEGQERTAHSPLTGHSNVVSPPSNGPSQSRHR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-12-02" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LMT0</accession>
    <name>Q1LMT0_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Outer membrane usher protein( fimbrial usher protein)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">papC</name>
        <name evidence="12" type="ordered locus">Rmet_1665</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell outer membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the fimbrial export usher family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08546.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1665"/>
    <dbReference type="EnsemblBacteria" id="ABF08546">
        <property type="protein sequence ID" value="ABF08546"/>
        <property type="gene ID" value="Rmet_1665"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1665"/>
    <dbReference type="eggNOG" id="COG3188">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009120_1_0_4"/>
    <dbReference type="OMA" id="WNNIRTY"/>
    <dbReference type="OrthoDB" id="120032at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009279">
        <property type="term" value="C:cell outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015473">
        <property type="term" value="F:fimbrial usher porin activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009297">
        <property type="term" value="P:pilus assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.2070">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.2610">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.410">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000015">
        <property type="entry name" value="Fimb_usher"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042186">
        <property type="entry name" value="FimD_plug_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025949">
        <property type="entry name" value="PapC-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043142">
        <property type="entry name" value="PapC-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025885">
        <property type="entry name" value="PapC_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037224">
        <property type="entry name" value="PapC_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30451">
        <property type="entry name" value="PTHR30451"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13953">
        <property type="entry name" value="PapC_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13954">
        <property type="entry name" value="PapC_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00577">
        <property type="entry name" value="Usher"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF141729">
        <property type="entry name" value="SSF141729"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0998">Cell outer membrane</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1134">Transmembrane beta strand</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="domain" description="PapC_N" evidence="11">
        <location>
            <begin position="59"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="domain" description="PapC_C" evidence="10">
        <location>
            <begin position="820"/>
            <end position="884"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004571"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF13953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF13954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ABF08546.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="929" mass="99055" checksum="E71B970AE3939330" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-10-07" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LMV1</accession>
    <name>Q1LMV1_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_1642</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08525.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1642"/>
    <dbReference type="EnsemblBacteria" id="ABF08525">
        <property type="protein sequence ID" value="ABF08525"/>
        <property type="gene ID" value="Rmet_1642"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1642"/>
    <dbReference type="HOGENOM" id="CLU_2247854_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="53"/>
            <end position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF08525.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="104" mass="11806" checksum="9BFD7819404BC0BC" modified="2006-05-30" version="1">MRLDRHTENFRRFPAGFRSSLTYNPFPSGPPRLHDRIMFRTLAMRSQPLPVKIMLTLTAIVAYMLACIVALAFGARWVLKNDPLLKQQQLLDSAAGARAEDHPT</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LMW4</accession>
    <name>Q1LMW4_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Glutathione-dependent formaldehyde-activating enzyme, GFA</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Rmet_1629</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the Gfa family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08512.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516357.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1629"/>
    <dbReference type="EnsemblBacteria" id="ABF08512">
        <property type="protein sequence ID" value="ABF08512"/>
        <property type="gene ID" value="Rmet_1629"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1629"/>
    <dbReference type="eggNOG" id="COG3791">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_116419_0_0_4"/>
    <dbReference type="OMA" id="LEGSCQC"/>
    <dbReference type="OrthoDB" id="1555973at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016846">
        <property type="term" value="F:carbon-sulfur lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006913">
        <property type="entry name" value="CENP-V/GFA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011057">
        <property type="entry name" value="Mss4-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04828">
        <property type="entry name" value="GFA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51316">
        <property type="entry name" value="SSF51316"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51891">
        <property type="entry name" value="CENP_V_GFA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CENP-V/GFA" evidence="3">
        <location>
            <begin position="3"/>
            <end position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005495"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51891"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF08512.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="161" mass="17931" checksum="D98C48C3ECF261C4" modified="2006-05-30" version="1">MHLEGSCQCGAVRFSLESDSPYPFMHCHCSICRKTGGGGGYGINLGGDASTLRVRGKTHVGRFHAVIREPGKRPVRSKAFRHFCVKCGSPLWLWDPRWPELVHPNASAIDTPLPKPPEVIEAALDYAAPWVDVPRGKGHRHARTWPAESLREWHERHGLLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="95" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LN91</accession>
    <name>Q1LN91_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">RuBisCO operon transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">cbbR1</name>
        <name evidence="3" type="ordered locus">Rmet_1502</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the LysR transcriptional regulatory family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08385.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516248.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1502"/>
    <dbReference type="EnsemblBacteria" id="ABF08385">
        <property type="protein sequence ID" value="ABF08385"/>
        <property type="gene ID" value="Rmet_1502"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1502"/>
    <dbReference type="eggNOG" id="COG0583">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039613_6_1_4"/>
    <dbReference type="OMA" id="LYCLKER"/>
    <dbReference type="OrthoDB" id="750230at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005119">
        <property type="entry name" value="LysR_subst-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000847">
        <property type="entry name" value="Tscrpt_reg_HTH_LysR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00126">
        <property type="entry name" value="HTH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03466">
        <property type="entry name" value="LysR_substrate"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00039">
        <property type="entry name" value="HTHLYSR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50931">
        <property type="entry name" value="HTH_LYSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH lysR-type" evidence="2">
        <location>
            <begin position="3"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF08385.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="293" mass="32213" checksum="4FD28A433788A9D4" modified="2006-05-30" version="1">MHLSLRQIRIFEAVARHRSYTRAAEELRLTQPAVFTQAKQLEEGVGHPLLERIGKQIYLTDAGREVLATCRETIKGLDCLEMRLADMQGLKRGRLRLAMVTTAEYLLPRLLGEFCAHYPGIEAKLIVSNREQLLARIANNEDDLTILGAPPEGMDVAAIPIADNPLVVIARNDHPLAGEKAIPMARIAEEPFVFREPGSGTRLATERHFAEHGHKLKVRLELGSNEAIKQAIAGGLGVSVLSGYTLALEGLSGLVQPLDVQGFPLMRKWYVAYPKGKHLSAVAEAFLGHLLGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="100" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LNA3</accession>
    <name>Q1LNA3_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_1490</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08373.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1490"/>
    <dbReference type="EnsemblBacteria" id="ABF08373">
        <property type="protein sequence ID" value="ABF08373"/>
        <property type="gene ID" value="Rmet_1490"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1490"/>
    <dbReference type="eggNOG" id="COG2207">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_87_0_4"/>
    <dbReference type="OMA" id="HARGQLF"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018060">
        <property type="entry name" value="HTH_AraC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018062">
        <property type="entry name" value="HTH_AraC-typ_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020449">
        <property type="entry name" value="Tscrpt_reg_HTH_AraC-type"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12833">
        <property type="entry name" value="HTH_18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00032">
        <property type="entry name" value="HTHARAC"/>
    </dbReference>
    <dbReference type="SMART" id="SM00342">
        <property type="entry name" value="HTH_ARAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00041">
        <property type="entry name" value="HTH_ARAC_FAMILY_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01124">
        <property type="entry name" value="HTH_ARAC_FAMILY_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH araC/xylS-type" evidence="2">
        <location>
            <begin position="89"/>
            <end position="186"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS01124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF08373.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="197" mass="21524" checksum="54F431E431126F0E" modified="2006-05-30" version="1">MTHSARVHGTMSGWVGHLAPELSLDLPGRPTAFRQSALSAAILERLPGSGVSSASDKRELRLLEALVDELGLLPPQPMCLPYPKTSRLADIARKIIDDLAVANSLDDMAALAFMSRRSLTRQFRAETGMSIVEWRDAARMQRAVQLLAAGHSVTSIAYTLGYDGASRFIDKFSATFGVTPKQFSLRSIRNPLREDAD</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-10-31" modified="2022-05-25" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LNE5</accession>
    <name>LPXB_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Lipid-A-disaccharide synthase</fullName>
            <ecNumber evidence="1">2.4.1.182</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">lpxB</name>
        <name type="ordered locus">Rmet_1448</name>
    </gene>
    <organism>
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Condensation of UDP-2,3-diacylglucosamine and 2,3-diacylglucosamine-1-phosphate to form lipid A disaccharide, a precursor of lipid A, a phosphorylated glycolipid that anchors the lipopolysaccharide to the outer membrane of the cell.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a lipid X + a UDP-2-N,3-O-bis[(3R)-3-hydroxyacyl]-alpha-D-glucosamine = a lipid A disaccharide + H(+) + UDP</text>
            <dbReference type="Rhea" id="RHEA:67828"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:137748"/>
            <dbReference type="ChEBI" id="CHEBI:176338"/>
            <dbReference type="ChEBI" id="CHEBI:176343"/>
            <dbReference type="EC" id="2.4.1.182"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Bacterial outer membrane biogenesis; LPS lipid A biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the LpxB family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.182" evidence="1"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08331.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516197.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q1LNE5"/>
    <dbReference type="SMR" id="Q1LNE5"/>
    <dbReference type="STRING" id="266264.Rmet_1448"/>
    <dbReference type="CAZy" id="GT19">
        <property type="family name" value="Glycosyltransferase Family 19"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF08331">
        <property type="protein sequence ID" value="ABF08331"/>
        <property type="gene ID" value="Rmet_1448"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1448"/>
    <dbReference type="eggNOG" id="COG0763">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036577_3_0_4"/>
    <dbReference type="OMA" id="PTVWAWR"/>
    <dbReference type="OrthoDB" id="1258510at2"/>
    <dbReference type="UniPathway" id="UPA00973"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008915">
        <property type="term" value="F:lipid-A-disaccharide synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009245">
        <property type="term" value="P:lipid A biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00392">
        <property type="entry name" value="LpxB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003835">
        <property type="entry name" value="Glyco_trans_19"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30372">
        <property type="entry name" value="PTHR30372"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02684">
        <property type="entry name" value="LpxB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00215">
        <property type="entry name" value="lpxB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0328">Glycosyltransferase</keyword>
    <keyword id="KW-0441">Lipid A biosynthesis</keyword>
    <keyword id="KW-0444">Lipid biosynthesis</keyword>
    <keyword id="KW-0443">Lipid metabolism</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000255212" description="Lipid-A-disaccharide synthase">
        <location>
            <begin position="1"/>
            <end position="401"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00392"/>
        </source>
    </evidence>
    <sequence length="401" mass="43417" checksum="C02EB9CE4BB11AAB" modified="2006-05-30" version="1">MVDTVQAVLPADAGANGNKRGSIAMVAGEASGDLLASLLLEGLRARLGESVDYAGIGGHRMMAQGFVSHWPMETLSVNGYVEVLGSLREILATRREIREQLLARPPLCFIGVDAPDFNFGLEVPLRRAGIPVVHFVSPSIWAWRGGRIRTIARAVDHILCLFPFEPEIYAKAGIPATYVGHPLADVIPMVPDVAGARAKLALPEGKRIVAVLPGSRQSEVRNLGATFFAAMDRMHRMDGNLAFVLPVASAPLREIVAGLHAQYPDIDLTVVDGQSHLAMESADVVLLASGTATLEAALYKKPMVISYKVPWLTAQIMKRQGYLPYVGLPNILSGRFVVPELLQDDATPEALARETLLQLNDEGNIAFLRQHFTTMHETLKRDTAKLAAGVVVDLMRSRGVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="92" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LNF0</accession>
    <name>Q1LNF0_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Outer membrane protein assembly factor BamA</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">yaeT</name>
        <name evidence="6" type="synonym">bamA</name>
        <name evidence="8" type="ordered locus">Rmet_1443</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Part of the outer membrane protein assembly complex, which is involved in assembly and insertion of beta-barrel proteins into the outer membrane.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Part of the Bam complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Cell outer membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the BamA family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08326.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1443"/>
    <dbReference type="EnsemblBacteria" id="ABF08326">
        <property type="protein sequence ID" value="ABF08326"/>
        <property type="gene ID" value="Rmet_1443"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1443"/>
    <dbReference type="eggNOG" id="COG4775">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007664_1_0_4"/>
    <dbReference type="OMA" id="IFMPAFG"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009279">
        <property type="term" value="C:cell outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043165">
        <property type="term" value="P:Gram-negative-bacterium-type cell outer membrane assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051205">
        <property type="term" value="P:protein insertion into membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01430">
        <property type="entry name" value="OM_assembly_BamA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000184">
        <property type="entry name" value="Bac_surfAg_D15"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010827">
        <property type="entry name" value="BamA/TamA_POTRA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039910">
        <property type="entry name" value="D15-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023707">
        <property type="entry name" value="OM_assembly_BamA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034746">
        <property type="entry name" value="POTRA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12815">
        <property type="entry name" value="PTHR12815"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12815:SF23">
        <property type="entry name" value="PTHR12815:SF23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01103">
        <property type="entry name" value="Omp85"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07244">
        <property type="entry name" value="POTRA"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006076">
        <property type="entry name" value="OM_assembly_OMP85"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03303">
        <property type="entry name" value="OM_YaeT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51779">
        <property type="entry name" value="POTRA"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0998">Cell outer membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2 6" id="KW-1134">Transmembrane beta strand</keyword>
    <feature type="domain" description="POTRA" evidence="7">
        <location>
            <begin position="52"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="domain" description="POTRA" evidence="7">
        <location>
            <begin position="120"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="domain" description="POTRA" evidence="7">
        <location>
            <begin position="203"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="domain" description="POTRA" evidence="7">
        <location>
            <begin position="294"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="domain" description="POTRA" evidence="7">
        <location>
            <begin position="375"/>
            <end position="449"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004442"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51779"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ABF08326.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="791" mass="88361" checksum="A5F9E83A1BDC845A" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LNK3</accession>
    <name>Q1LNK3_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 7">Malate synthase</fullName>
            <ecNumber evidence="2 7">2.3.3.9</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">aceB</name>
        <name evidence="8" type="ordered locus">Rmet_1390</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 7">
            <text>acetyl-CoA + glyoxylate + H2O = (S)-malate + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:18181"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15589"/>
            <dbReference type="ChEBI" id="CHEBI:36655"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="EC" id="2.3.3.9"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="7">Carbohydrate metabolism; glyoxylate cycle; (S)-malate from isocitrate: step 2/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the malate synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.3.9" evidence="2 7"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08273.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516149.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1390"/>
    <dbReference type="EnsemblBacteria" id="ABF08273">
        <property type="protein sequence ID" value="ABF08273"/>
        <property type="gene ID" value="Rmet_1390"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1390"/>
    <dbReference type="eggNOG" id="COG2225">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018928_3_0_4"/>
    <dbReference type="OMA" id="CHKRGAP"/>
    <dbReference type="OrthoDB" id="322024at2"/>
    <dbReference type="UniPathway" id="UPA00703">
        <property type="reaction ID" value="UER00720"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004474">
        <property type="term" value="F:malate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006097">
        <property type="term" value="P:glyoxylate cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00727">
        <property type="entry name" value="malate_synt_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1220.12">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044856">
        <property type="entry name" value="Malate_synth_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011076">
        <property type="entry name" value="Malate_synth_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006252">
        <property type="entry name" value="Malate_synthA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001465">
        <property type="entry name" value="Malate_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019830">
        <property type="entry name" value="Malate_synthase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046363">
        <property type="entry name" value="MS_N_TIM-barrel_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42902">
        <property type="entry name" value="PTHR42902"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01274">
        <property type="entry name" value="Malate_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001363">
        <property type="entry name" value="Malate_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51645">
        <property type="entry name" value="SSF51645"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01344">
        <property type="entry name" value="malate_syn_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00510">
        <property type="entry name" value="MALATE_SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="3 7" id="KW-0329">Glyoxylate bypass</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7 8" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 7" id="KW-0816">Tricarboxylic acid cycle</keyword>
    <feature type="active site" description="Proton acceptor" evidence="6">
        <location>
            <position position="163"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="6">
        <location>
            <position position="445"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012636"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR001363-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ABF08273.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="527" mass="58842" checksum="9E8D0359D5C1504B" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LNV5</accession>
    <name>Q1LNV5_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Hydrogenase maturation factor HypA</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">hypA</name>
        <name evidence="5" type="synonym">hypA1</name>
        <name evidence="5" type="ordered locus">Rmet_1286</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Involved in the maturation of [NiFe] hydrogenases. Required for nickel insertion into the metal center of the hydrogenase.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the HypA/HybF family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08171.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516053.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1286"/>
    <dbReference type="EnsemblBacteria" id="ABF08171">
        <property type="protein sequence ID" value="ABF08171"/>
        <property type="gene ID" value="Rmet_1286"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1286"/>
    <dbReference type="eggNOG" id="COG0375">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_126929_0_0_4"/>
    <dbReference type="OMA" id="HEMTICE"/>
    <dbReference type="OrthoDB" id="1995603at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016151">
        <property type="term" value="F:nickel cation binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051604">
        <property type="term" value="P:protein maturation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00213">
        <property type="entry name" value="HypA_HybF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020538">
        <property type="entry name" value="Hydgase_Ni_incorp_HypA/HybF_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000688">
        <property type="entry name" value="HypA/HybF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34535">
        <property type="entry name" value="PTHR34535"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01155">
        <property type="entry name" value="HypA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004761">
        <property type="entry name" value="Hydrgn_mat_HypA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01249">
        <property type="entry name" value="HYPA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1 4" id="KW-0533">Nickel</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0862">Zinc</keyword>
    <feature type="binding site" evidence="4">
        <location>
            <position position="2"/>
        </location>
        <ligand>
            <name>Ni(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:49786"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="73"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="76"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="89"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="92"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022596"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00213"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABF08171.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="115" mass="12211" checksum="B5E7210EBB58FDE1" modified="2006-05-30" version="1">MHEASLAGGVLQLVEDTARREGFARLLELRLEAGQLAGVDVRALRFALESIAPGTLMAGARIEIEEPPGQAWCLSCSQTVAIAQRGDACPACGGYQLQPTGGMELRIIDMKVSDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LP89</accession>
    <name>Q1LP89_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Thioesterase superfamily</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_1151</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08037.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515938.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1151"/>
    <dbReference type="EnsemblBacteria" id="ABF08037">
        <property type="protein sequence ID" value="ABF08037"/>
        <property type="gene ID" value="Rmet_1151"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1151"/>
    <dbReference type="eggNOG" id="COG0824">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_101141_2_2_4"/>
    <dbReference type="OMA" id="DMDAMGH"/>
    <dbReference type="OrthoDB" id="1989068at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029069">
        <property type="entry name" value="HotDog_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006683">
        <property type="entry name" value="Thioestr_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03061">
        <property type="entry name" value="4HBT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54637">
        <property type="entry name" value="SSF54637"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="4HBT" evidence="1">
        <location>
            <begin position="19"/>
            <end position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF08037.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="133" mass="15162" checksum="D8F475B938C5914C" modified="2006-05-30" version="1">MKHVFTFVMPIRWGDMDAMGHVNNTVYFRYMEQARLEWFASLGRSGKDASGHGPVIINASMTFLKQLRHPGDIECRVYAGKLGRTSFETWTEIIRTDLPDVIWAEGGAKVVWCDYAAEKSVPVPDEIRRLIEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="65" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LPA8</accession>
    <name>Q1LPA8_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Predicted membrane protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_1132</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08018.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515919.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1132"/>
    <dbReference type="DNASU" id="4037930"/>
    <dbReference type="EnsemblBacteria" id="ABF08018">
        <property type="protein sequence ID" value="ABF08018"/>
        <property type="gene ID" value="Rmet_1132"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1132"/>
    <dbReference type="eggNOG" id="COG4323">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_140388_0_0_4"/>
    <dbReference type="OMA" id="FRHPLYS"/>
    <dbReference type="OrthoDB" id="1928487at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009305">
        <property type="entry name" value="Mpo1-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06127">
        <property type="entry name" value="DUF962"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="31"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="54"/>
            <end position="73"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF08018.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="105" mass="12168" checksum="75659D0609D1C320" modified="2006-05-30" version="1">MAHAHTKEFTSFSDFYPFYLNEHRNRTCRRLHFVGSTVALACLVTLLATGNAWWLLAALVSGYAFAWVGHFGFEKNRPATFKHPFYSLMGDWVMYADILRGKIRF</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-05-20" modified="2022-08-03" version="98" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LPG5</accession>
    <name>MUTS_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DNA mismatch repair protein MutS</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">mutS</name>
        <name type="ordered locus">Rmet_1075</name>
    </gene>
    <organism>
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">This protein is involved in the repair of mismatches in DNA. It is possible that it carries out the mismatch recognition step. This protein has a weak ATPase activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the DNA mismatch repair MutS family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07961.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q1LPG5"/>
    <dbReference type="SMR" id="Q1LPG5"/>
    <dbReference type="STRING" id="266264.Rmet_1075"/>
    <dbReference type="EnsemblBacteria" id="ABF07961">
        <property type="protein sequence ID" value="ABF07961"/>
        <property type="gene ID" value="Rmet_1075"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1075"/>
    <dbReference type="eggNOG" id="COG0249">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002472_3_1_4"/>
    <dbReference type="OMA" id="TPMMAQY"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140664">
        <property type="term" value="F:ATP-dependent DNA damage sensor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003684">
        <property type="term" value="F:damaged DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030983">
        <property type="term" value="F:mismatched DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006298">
        <property type="term" value="P:mismatch repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1170.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00096">
        <property type="entry name" value="MutS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005748">
        <property type="entry name" value="DNA_mismatch_repair_MutS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007695">
        <property type="entry name" value="DNA_mismatch_repair_MutS-lik_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017261">
        <property type="entry name" value="DNA_mismatch_repair_MutS/MSH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000432">
        <property type="entry name" value="DNA_mismatch_repair_MutS_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007861">
        <property type="entry name" value="DNA_mismatch_repair_MutS_clamp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007696">
        <property type="entry name" value="DNA_mismatch_repair_MutS_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016151">
        <property type="entry name" value="DNA_mismatch_repair_MutS_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036187">
        <property type="entry name" value="DNA_mismatch_repair_MutS_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007860">
        <property type="entry name" value="DNA_mmatch_repair_MutS_con_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036678">
        <property type="entry name" value="MutS_con_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045076">
        <property type="entry name" value="MutS_family"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11361">
        <property type="entry name" value="PTHR11361"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01624">
        <property type="entry name" value="MutS_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05188">
        <property type="entry name" value="MutS_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05192">
        <property type="entry name" value="MutS_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05190">
        <property type="entry name" value="MutS_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00488">
        <property type="entry name" value="MutS_V"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037677">
        <property type="entry name" value="DNA_mis_repair_Msh6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00534">
        <property type="entry name" value="MUTSac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00533">
        <property type="entry name" value="MUTSd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48334">
        <property type="entry name" value="SSF48334"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53150">
        <property type="entry name" value="SSF53150"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55271">
        <property type="entry name" value="SSF55271"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01070">
        <property type="entry name" value="mutS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00486">
        <property type="entry name" value="DNA_MISMATCH_REPAIR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0227">DNA damage</keyword>
    <keyword id="KW-0234">DNA repair</keyword>
    <keyword id="KW-0238">DNA-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000335209" description="DNA mismatch repair protein MutS">
        <location>
            <begin position="1"/>
            <end position="916"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="843"/>
            <end position="880"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="843"/>
            <end position="860"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="656"/>
            <end position="663"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00096"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="916" mass="99842" checksum="542A42985B7D2A44" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="65" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LPQ6</accession>
    <name>Q1LPQ6_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_0984</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07870.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008641734.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0984"/>
    <dbReference type="EnsemblBacteria" id="ABF07870">
        <property type="protein sequence ID" value="ABF07870"/>
        <property type="gene ID" value="Rmet_0984"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0984"/>
    <dbReference type="eggNOG" id="COG3825">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_059555_0_0_4"/>
    <dbReference type="OMA" id="VWDKREF"/>
    <dbReference type="OrthoDB" id="1121563at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008912">
        <property type="entry name" value="Uncharacterised_CoxE"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05762">
        <property type="entry name" value="VWA_CoxE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF07870.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="391" mass="45376" checksum="F65050C7991D64AF" modified="2006-05-30" version="1">MLIDFFFSLRHAKLPVSVKEYLTMLEALRAQVIAPSIDEFYYLSRMTLVKDEKHFDKFDQTFAAYFKGVESLIDWKSDIPLDWLQKTLERELSAEEKAKIEAMGGLDKLMERLKQLLEEQKEKHEGGNKWIGTGGTSPFGHGGYNPEGIRIGGPSKGNRTAIKVWETRAYKDYDDQVELGTRNIKVALRRLRRFAREGADTELDLDDTIHSTAANAGMLDIKLRPERHNKVKVLMLMDVGGSMDDHIKRVEELFSATKTEFKHLEYYYFHNCLYDYVWKNNRRRHAEKIATWDLIHKYTPDYKVIFVGDATMSPYEILQPGGSVEYNNAEAGAVWLNRMIEQFPRFVWLNPEPEGLWQYRQSITVINQIMKNRMYPVTIAGLEAAMKVLSK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-06-10" modified="2022-05-25" version="72" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LPT3</accession>
    <name>URED_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Urease accessory protein UreD</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">ureD</name>
        <name type="ordered locus">Rmet_0957</name>
    </gene>
    <organism>
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Required for maturation of urease via the functional incorporation of the urease nickel metallocenter.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">UreD, UreF and UreG form a complex that acts as a GTP-hydrolysis-dependent molecular chaperone, activating the urease apoprotein by helping to assemble the nickel containing metallocenter of UreC. The UreE protein probably delivers the nickel.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the UreD family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07843.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515765.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q1LPT3"/>
    <dbReference type="SMR" id="Q1LPT3"/>
    <dbReference type="STRING" id="266264.Rmet_0957"/>
    <dbReference type="EnsemblBacteria" id="ABF07843">
        <property type="protein sequence ID" value="ABF07843"/>
        <property type="gene ID" value="Rmet_0957"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0957"/>
    <dbReference type="eggNOG" id="COG0829">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_056339_0_0_4"/>
    <dbReference type="OMA" id="CQHIIVH"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016151">
        <property type="term" value="F:nickel cation binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006807">
        <property type="term" value="P:nitrogen compound metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01384">
        <property type="entry name" value="UreD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002669">
        <property type="entry name" value="UreD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33643">
        <property type="entry name" value="PTHR33643"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01774">
        <property type="entry name" value="UreD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0143">Chaperone</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0996">Nickel insertion</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000340499" description="Urease accessory protein UreD">
        <location>
            <begin position="1"/>
            <end position="293"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01384"/>
        </source>
    </evidence>
    <sequence length="293" mass="31470" checksum="F1EA71F8C6677C55" modified="2006-05-30" version="1">MRHPDFPSQLAMTSAWHASLRLRFAARAGRTAMVERRHEGPLRVQKPLYPEGEICHGVVLHPPAGVAGGDRLDIDIAVASGAHAVLATPGATKWYKSLGRDAAQHVRIEVAAGGKLDWLPQENIVFDDARARITTDVVVAPGGSAIGWDAVVLGRQASGERWASGALWLDTRVGDPDRALWIEQSHFDGASPLRGAVAGMDGLHILGTLWAIGPGATQELAEALAEHLPYRADLRAGVTCLAGYGTATAQSMLLLRVLGSDMEAVRHLMIDAWTVLRQPMHGVPARPLRLWST</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="115" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LQ31</accession>
    <name>Q1LQ31_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transcription elongation factor GreB</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">Transcript cleavage factor GreB</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="4 8" type="primary">greB</name>
        <name evidence="8" type="ordered locus">Rmet_0859</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Necessary for efficient RNA polymerase transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary complexes. Cleavage of the nascent transcript by cleavage factors such as GreA or GreB allows the resumption of elongation from the new 3'terminus. GreB releases sequences of up to 9 nucleotides in length.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the GreA/GreB family. GreB subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07745.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515686.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0859"/>
    <dbReference type="EnsemblBacteria" id="ABF07745">
        <property type="protein sequence ID" value="ABF07745"/>
        <property type="gene ID" value="Rmet_0859"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0859"/>
    <dbReference type="eggNOG" id="COG0782">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_101379_3_0_4"/>
    <dbReference type="OMA" id="DQIFFGA"/>
    <dbReference type="OrthoDB" id="1591985at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070063">
        <property type="term" value="F:RNA polymerase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032784">
        <property type="term" value="P:regulation of DNA-templated transcription, elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.180">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.50.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00105">
        <property type="entry name" value="GreA_GreB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00930">
        <property type="entry name" value="GreB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036953">
        <property type="entry name" value="GreA/GreB_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018151">
        <property type="entry name" value="TF_GreA/GreB_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028624">
        <property type="entry name" value="Tscrpt_elong_fac_GreA/B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001437">
        <property type="entry name" value="Tscrpt_elong_fac_GreA/B_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023459">
        <property type="entry name" value="Tscrpt_elong_fac_GreA/B_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022691">
        <property type="entry name" value="Tscrpt_elong_fac_GreA/B_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036805">
        <property type="entry name" value="Tscrpt_elong_fac_GreA/B_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006358">
        <property type="entry name" value="Tscrpt_elong_fac_GreB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30437">
        <property type="entry name" value="PTHR30437"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01272">
        <property type="entry name" value="GreA_GreB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03449">
        <property type="entry name" value="GreA_GreB_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006092">
        <property type="entry name" value="GreA_GreB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46557">
        <property type="entry name" value="SSF46557"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01461">
        <property type="entry name" value="greB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00829">
        <property type="entry name" value="GREAB_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00830">
        <property type="entry name" value="GREAB_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8" id="KW-0251">Elongation factor</keyword>
    <keyword evidence="8" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0804">Transcription</keyword>
    <keyword evidence="1 4" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="GreA_GreB_N" evidence="7">
        <location>
            <begin position="30"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="domain" description="GreA_GreB" evidence="6">
        <location>
            <begin position="109"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00930"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF03449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ABF07745.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="186" mass="20901" checksum="BC02CB50C9CAE911" modified="2006-05-30" version="1">MNKAFVKESSNDDDEDLPEGATPLPAGTKNYITAAGYKRLRDELMHLIDVERPDIVQIVSWAASNGDRSENGDYLYGKKRMREIDRRIRFLTRRLDKAEVVDPSMQGDNDQIFFGATVTYANQSGEETTITIVGMDEVDLDSNHVSWISPIAKALIKAREGDTVPLRTPAGIEQIDILEVRYPQKS</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-12-12" modified="2022-08-03" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LQ34</accession>
    <name>KGUA_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Guanylate kinase</fullName>
            <ecNumber evidence="1">2.7.4.8</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">GMP kinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">gmk</name>
        <name type="ordered locus">Rmet_0856</name>
    </gene>
    <organism>
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Essential for recycling GMP and indirectly, cGMP.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + GMP = ADP + GDP</text>
            <dbReference type="Rhea" id="RHEA:20780"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58115"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.8"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the guanylate kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.4.8" evidence="1"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07742.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515683.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q1LQ34"/>
    <dbReference type="SMR" id="Q1LQ34"/>
    <dbReference type="STRING" id="266264.Rmet_0856"/>
    <dbReference type="EnsemblBacteria" id="ABF07742">
        <property type="protein sequence ID" value="ABF07742"/>
        <property type="gene ID" value="Rmet_0856"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0856"/>
    <dbReference type="eggNOG" id="COG0194">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001715_1_0_4"/>
    <dbReference type="OMA" id="EWAVVHG"/>
    <dbReference type="OrthoDB" id="1502854at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004385">
        <property type="term" value="F:guanylate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00328">
        <property type="entry name" value="Guanylate_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008145">
        <property type="entry name" value="GK/Ca_channel_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008144">
        <property type="entry name" value="Guanylate_kin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017665">
        <property type="entry name" value="Guanylate_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020590">
        <property type="entry name" value="Guanylate_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00625">
        <property type="entry name" value="Guanylate_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00072">
        <property type="entry name" value="GuKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03263">
        <property type="entry name" value="guanyl_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00856">
        <property type="entry name" value="GUANYLATE_KINASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50052">
        <property type="entry name" value="GUANYLATE_KINASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0418">Kinase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000266378" description="Guanylate kinase">
        <location>
            <begin position="1"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="domain" description="Guanylate kinase-like" evidence="1">
        <location>
            <begin position="15"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="22"/>
            <end position="29"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00328"/>
        </source>
    </evidence>
    <sequence length="216" mass="24340" checksum="76F4B7104334A445" modified="2006-05-30" version="1">MSETTHTAIDTAYPGNLFMVVAPSGAGKSTLVNALLAQDKAIRLSISHTTRSPRPGEQNGREYHFISVDEFRAARDRGEFLEWAEVHGNYYATSRVWIEEQMAQGTDVLLEIDWQGAQQVHKRFSNAVEIFILPPSLTALEERLKKRGQDEPNVIVRRLLAAGSEMSHASESDYVIINEVFDDALKQLQNVVHATRLRFSSQKARHAELFIELGIH</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-12-12" modified="2022-05-25" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LQB1</accession>
    <name>NTPPA_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">dTTP/UTP pyrophosphatase</fullName>
            <shortName evidence="1">dTTPase/UTPase</shortName>
            <ecNumber evidence="1">3.6.1.9</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Nucleoside triphosphate pyrophosphatase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Nucleotide pyrophosphatase</fullName>
            <shortName evidence="1">Nucleotide PPase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name type="ordered locus">Rmet_0779</name>
    </gene>
    <organism>
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Nucleoside triphosphate pyrophosphatase that hydrolyzes dTTP and UTP. May have a dual role in cell division arrest and in preventing the incorporation of modified nucleotides into cellular nucleic acids.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>dTTP + H2O = diphosphate + dTMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:28534"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37568"/>
            <dbReference type="ChEBI" id="CHEBI:63528"/>
            <dbReference type="EC" id="3.6.1.9"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>H2O + UTP = diphosphate + H(+) + UMP</text>
            <dbReference type="Rhea" id="RHEA:29395"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:46398"/>
            <dbReference type="ChEBI" id="CHEBI:57865"/>
            <dbReference type="EC" id="3.6.1.9"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the Maf family. YhdE subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.1.9" evidence="1"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07665.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515619.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q1LQB1"/>
    <dbReference type="SMR" id="Q1LQB1"/>
    <dbReference type="STRING" id="266264.Rmet_0779"/>
    <dbReference type="EnsemblBacteria" id="ABF07665">
        <property type="protein sequence ID" value="ABF07665"/>
        <property type="gene ID" value="Rmet_0779"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0779"/>
    <dbReference type="eggNOG" id="COG0424">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_040416_2_1_4"/>
    <dbReference type="OMA" id="RIDGDFY"/>
    <dbReference type="OrthoDB" id="1469203at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036218">
        <property type="term" value="F:dTTP diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035529">
        <property type="term" value="F:NADH pyrophosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036221">
        <property type="term" value="F:UTP diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009117">
        <property type="term" value="P:nucleotide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00555">
        <property type="entry name" value="Maf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.950.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00528">
        <property type="entry name" value="Maf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029001">
        <property type="entry name" value="ITPase-like_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003697">
        <property type="entry name" value="Maf-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43213">
        <property type="entry name" value="PTHR43213"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02545">
        <property type="entry name" value="Maf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006305">
        <property type="entry name" value="Maf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52972">
        <property type="entry name" value="SSF52972"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00172">
        <property type="entry name" value="maf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0546">Nucleotide metabolism</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000267389" description="dTTP/UTP pyrophosphatase">
        <location>
            <begin position="1"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="1">
        <location>
            <position position="81"/>
        </location>
    </feature>
    <feature type="site" description="Important for substrate specificity" evidence="1">
        <location>
            <position position="13"/>
        </location>
    </feature>
    <feature type="site" description="Important for substrate specificity" evidence="1">
        <location>
            <position position="82"/>
        </location>
    </feature>
    <feature type="site" description="Important for substrate specificity" evidence="1">
        <location>
            <position position="164"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00528"/>
        </source>
    </evidence>
    <sequence length="200" mass="21229" checksum="736D42F5912D962D" modified="2006-05-30" version="1">MTSILYLASQSPRRRELLTQLGVTYELLLADAGEDAEALEAVRPGESPDDYVQRVCALKADAALQRRARRGLPDAPILTSDTTVCRGGDILGKPADARDAAAMLASLSGTTHRVLTAVTVATSAGQRHALSISHVTFRPILAPEIERYVASGEPLGKAGAYGIQGRAAEFVERIDGSYSGIMGLPLFETAALLREAGLHF</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LQG8</accession>
    <name>Q1LQG8_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 4">30S ribosomal protein S1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">rpsA</name>
        <name evidence="6" type="ordered locus">Rmet_0722</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Binds mRNA; thus facilitating recognition of the initiation point. It is needed to translate mRNA with a short Shine-Dalgarno (SD) purine-rich sequence.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the bacterial ribosomal protein bS1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07608.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008643658.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0722"/>
    <dbReference type="EnsemblBacteria" id="ABF07608">
        <property type="protein sequence ID" value="ABF07608"/>
        <property type="gene ID" value="Rmet_0722"/>
    </dbReference>
    <dbReference type="GeneID" id="60825209"/>
    <dbReference type="KEGG" id="rme:Rmet_0722"/>
    <dbReference type="eggNOG" id="COG0539">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015805_2_1_4"/>
    <dbReference type="OMA" id="SLNEFRY"/>
    <dbReference type="OrthoDB" id="1235756at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035104">
        <property type="entry name" value="Ribosomal_protein_S1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000110">
        <property type="entry name" value="Ribosomal_S1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022967">
        <property type="entry name" value="S1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003029">
        <property type="entry name" value="S1_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10724:SF7">
        <property type="entry name" value="PTHR10724:SF7"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00575">
        <property type="entry name" value="S1"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002111">
        <property type="entry name" value="RpsA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00681">
        <property type="entry name" value="RIBOSOMALS1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00316">
        <property type="entry name" value="S1"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00717">
        <property type="entry name" value="rpsA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50126">
        <property type="entry name" value="S1"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="4" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="S1 motif" evidence="5">
        <location>
            <begin position="26"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="domain" description="S1 motif" evidence="5">
        <location>
            <begin position="110"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="domain" description="S1 motif" evidence="5">
        <location>
            <begin position="197"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="domain" description="S1 motif" evidence="5">
        <location>
            <begin position="282"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="domain" description="S1 motif" evidence="5">
        <location>
            <begin position="369"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="domain" description="S1 motif" evidence="5">
        <location>
            <begin position="456"/>
            <end position="525"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00016782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRNR" id="PIRNR002111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABF07608.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="564" mass="61649" checksum="496F99106ACF6C00" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="67" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LR52</accession>
    <name>Q1LR52_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_0488</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07374.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515359.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0488"/>
    <dbReference type="EnsemblBacteria" id="ABF07374">
        <property type="protein sequence ID" value="ABF07374"/>
        <property type="gene ID" value="Rmet_0488"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0488"/>
    <dbReference type="eggNOG" id="ENOG5032RXQ">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1375933_0_0_4"/>
    <dbReference type="OMA" id="WYFQNGP"/>
    <dbReference type="OrthoDB" id="1766090at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021332">
        <property type="entry name" value="DUF2944"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11161">
        <property type="entry name" value="DUF2944"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF07374.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="190" mass="21606" checksum="2FC3630C0BF31E63" modified="2006-05-30" version="1">MDEVVKQAMARWPNVPYCYGWLALDRRGQWRMRNEFAQQHGLSGDPIRHEALIGFIERNYTHDERGAWYFQNGPQRVFVSLMYTPWVARLHNGRLTTTSDRPFTPTACYTDEQGNVVFAGTVQGVDNAVQVALLHDHDLEAFSEACDWHGEACGATLGVFHHDGVDIDIEPVTEAELPKRYGFVRTPVAE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-01-15" modified="2022-08-03" version="99" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LR74</accession>
    <name>RECA_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Protein RecA</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Recombinase A</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">recA</name>
        <name type="ordered locus">Rmet_0466</name>
    </gene>
    <organism>
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Can catalyze the hydrolysis of ATP in the presence of single-stranded DNA, the ATP-dependent uptake of single-stranded DNA by duplex DNA, and the ATP-dependent hybridization of homologous single-stranded DNAs. It interacts with LexA causing its activation and leading to its autocatalytic cleavage.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the RecA family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07352.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008646499.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q1LR74"/>
    <dbReference type="SMR" id="Q1LR74"/>
    <dbReference type="STRING" id="266264.Rmet_0466"/>
    <dbReference type="EnsemblBacteria" id="ABF07352">
        <property type="protein sequence ID" value="ABF07352"/>
        <property type="gene ID" value="Rmet_0466"/>
    </dbReference>
    <dbReference type="GeneID" id="60824071"/>
    <dbReference type="KEGG" id="rme:Rmet_0466"/>
    <dbReference type="eggNOG" id="COG0468">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_040469_3_2_4"/>
    <dbReference type="OMA" id="DYGEQAL"/>
    <dbReference type="OrthoDB" id="1080436at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140664">
        <property type="term" value="F:ATP-dependent DNA damage sensor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003684">
        <property type="term" value="F:damaged DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009432">
        <property type="term" value="P:SOS response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00983">
        <property type="entry name" value="recA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00268">
        <property type="entry name" value="RecA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013765">
        <property type="entry name" value="DNA_recomb/repair_RecA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020584">
        <property type="entry name" value="DNA_recomb/repair_RecA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020588">
        <property type="entry name" value="RecA_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023400">
        <property type="entry name" value="RecA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020587">
        <property type="entry name" value="RecA_monomer-monomer_interface"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45900">
        <property type="entry name" value="PTHR45900"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00154">
        <property type="entry name" value="RecA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00142">
        <property type="entry name" value="RECA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54752">
        <property type="entry name" value="SSF54752"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02012">
        <property type="entry name" value="tigrfam_recA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00321">
        <property type="entry name" value="RECA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50162">
        <property type="entry name" value="RECA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50163">
        <property type="entry name" value="RECA_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0227">DNA damage</keyword>
    <keyword id="KW-0233">DNA recombination</keyword>
    <keyword id="KW-0234">DNA repair</keyword>
    <keyword id="KW-0238">DNA-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0742">SOS response</keyword>
    <feature type="chain" id="PRO_1000047977" description="Protein RecA">
        <location>
            <begin position="1"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="75"/>
            <end position="82"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00268"/>
        </source>
    </evidence>
    <sequence length="353" mass="37483" checksum="3B883308EB294272" modified="2006-05-30" version="1">MDDKKAGAGVSAEKQKALAAALSQIEKQFGKGSIMRLGDGEIEQDIQVVSTGSLGLDIALGVGGLPRGRVVEIYGPESSGKTTLTLQVVAEMQKLGGTCAFIDAEHALDVQYAGKLGVDVGNLLISQPDTGEQALEITDALVRSGSIDLIVIDSVAALVPKAEIEGEMGDSLPGLQARLMSQALRKLTGTIKRTNCLVIFINQIRMKIGVMFGSPETTTGGNALKFYASVRLDIRRIGSIKKGDEVVGNETKVKVVKNKVSPPFREAFFDILYGQGISRQGEIIDLGVDAKIVEKAGAWYSYNGEKIGQGKDNAREYLRENPDIADEIENKVRLALGVAPLNTVAGAPAEVEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="102" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LR95</accession>
    <name>Q1LR95_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 5">D,D-heptose 1,7-bisphosphate phosphatase</fullName>
            <ecNumber evidence="5">3.1.3.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">gmhB</name>
        <name evidence="9" type="ordered locus">Rmet_0445</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 5">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the gmhB family.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.-" evidence="5"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07331.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515319.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0445"/>
    <dbReference type="EnsemblBacteria" id="ABF07331">
        <property type="protein sequence ID" value="ABF07331"/>
        <property type="gene ID" value="Rmet_0445"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0445"/>
    <dbReference type="eggNOG" id="COG0241">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_085077_2_0_4"/>
    <dbReference type="OMA" id="EHQICLE"/>
    <dbReference type="OrthoDB" id="1472357at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016791">
        <property type="term" value="F:phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006549">
        <property type="entry name" value="HAD-SF_hydro_IIIA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004446">
        <property type="entry name" value="Heptose_bisP_phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006543">
        <property type="entry name" value="Histidinol-phos"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42891">
        <property type="entry name" value="PTHR42891"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004682">
        <property type="entry name" value="GmhB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="SSF56784"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01662">
        <property type="entry name" value="HAD-SF-IIIA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01656">
        <property type="entry name" value="Histidinol-ppas"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="2 5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <feature type="active site" description="Nucleophile" evidence="6">
        <location>
            <position position="12"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="6">
        <location>
            <position position="14"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="12"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="14"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="94"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="96"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="102"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="104"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="131"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="site" description="Stabilizes the phosphoryl group" evidence="7">
        <location>
            <position position="55"/>
        </location>
    </feature>
    <feature type="site" description="Contributes to substrate recognition" evidence="7">
        <location>
            <position position="105"/>
        </location>
    </feature>
    <feature type="site" description="Stabilizes the phosphoryl group" evidence="7">
        <location>
            <position position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00031828"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRNR" id="PIRNR004682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR004682-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR004682-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR004682-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ABF07331.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="193" mass="21003" checksum="DB3B46BB83DB50CF" modified="2006-05-30" version="1">MPQSQLKFVILDRDGVINRDSDQFIKTPDEWIPIEGSLEAIAMLNQAGYRVVVASNQSGIGRGLFEMSALNAMHEKMYKALANLGGRVEAIFFCPHTAADNCDCRKPKSGLYEQIGERFGIELRTVPTVGDSLRDLQAGVAVGCIPHLVRTGKGMKTLEKGGLPDGTRVHDDLAAFARWLIDDAAPKPQPTAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="72" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LRG7</accession>
    <name>Q1LRG7_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Ubiquinone biosynthesis accessory factor UbiJ</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">ubiJ</name>
        <name evidence="4" type="ordered locus">Rmet_0373</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Required for ubiquinone (coenzyme Q) biosynthesis. Binds hydrophobic ubiquinone biosynthetic intermediates via its SCP2 domain and is essential for the stability of the Ubi complex. May constitute a docking platform where Ubi enzymes assemble and access their SCP2-bound polyprenyl substrates.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; ubiquinone biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the UbiJ family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07259.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515250.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0373"/>
    <dbReference type="EnsemblBacteria" id="ABF07259">
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        <property type="gene ID" value="Rmet_0373"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0373"/>
    <dbReference type="eggNOG" id="COG3165">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_100130_0_0_4"/>
    <dbReference type="OMA" id="LEQEPWA"/>
    <dbReference type="OrthoDB" id="1959286at2"/>
    <dbReference type="UniPathway" id="UPA00232"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032150">
        <property type="term" value="P:ubiquinone biosynthetic process from chorismate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02215">
        <property type="entry name" value="UbiJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003033">
        <property type="entry name" value="SCP2_sterol-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038989">
        <property type="entry name" value="UbiJ"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38693">
        <property type="entry name" value="PTHR38693"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02036">
        <property type="entry name" value="SCP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0831">Ubiquinone biosynthesis</keyword>
    <feature type="domain" description="SCP2" evidence="3">
        <location>
            <begin position="16"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="182"/>
            <end position="209"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02215"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF02036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF07259.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="223" mass="23821" checksum="531FA24A3E3DEC42" modified="2006-05-30" version="1">MNALPTPLAAPIVAALNHLIEQEPWACSLLTPFAGRVIRFDASAFALSLKVTEQGGIDQAPEAEAAAVTLIVPLQQWPLVVADVAEGGQAAAMRHVRIDGDAELANVVSTLARNLRWDAAEDLSRALRGVMGGPVSDSVAQRVVEGARQVHESATRAGRALLDNVTEYLLDEQPTLVRHAALEGFGDDLARLRDDLARLEKRLERLERAPGAGSPDALPSAHR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LRS1</accession>
    <name>Q1LRS1_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Cytochrome c oxidase assembly</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">ctaA</name>
        <name evidence="7" type="ordered locus">Rmet_0269</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07155.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515159.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0269"/>
    <dbReference type="EnsemblBacteria" id="ABF07155">
        <property type="protein sequence ID" value="ABF07155"/>
        <property type="gene ID" value="Rmet_0269"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0269"/>
    <dbReference type="eggNOG" id="COG1612">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041525_0_0_4"/>
    <dbReference type="OMA" id="HRYFAGT"/>
    <dbReference type="OrthoDB" id="1135592at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016653">
        <property type="term" value="F:oxidoreductase activity, acting on NAD(P)H, heme protein as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006784">
        <property type="term" value="P:heme A biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003780">
        <property type="entry name" value="COX15/CtaA_fam"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02628">
        <property type="entry name" value="COX15-CtaA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0350">Heme biosynthesis</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="6"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="34"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="100"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="131"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="158"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="197"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="269"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="299"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="323"/>
            <end position="343"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABF07155.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="367" mass="40441" checksum="567323B217DF00B9" modified="2006-05-30" version="1">MLLQLAIIGILIALLPLSYVMVRGDRDKYRRLVWITAFLTLDLIMFGSFTRLTDSGLGCPDWPGCYGHSNPHAAMEPIRAAETALPSGPVTLMKAWIEMIHRYFAMAVGVLIISLMVLAWVKRRELKQSPWFATGVFLLVCLQGAFGMWTVTMKLQPIIVVTHLLLAMALLASLILLGSRNEPPHVVAPQAASLRWAGLLALVLLVVQIFLGGWVSTNYAVLACTDFPLCNGQLVPEMDFRHGFTLWRQLGMTADGDYIPHAALVAIHWVHRGFAFVVLGFLLWFALRARRMEGLARHARWLLVVLTLQLCTGMSNIVFDWPLVAAVAHNGGAALLLMLLLRLNYNIGLATRSAGAATQPSIAARAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LRW8</accession>
    <name>Q1LRW8_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">2-nitropropane dioxygenase</fullName>
            <ecNumber evidence="2">1.13.11.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">nppD</name>
        <name evidence="2" type="ordered locus">Rmet_0222</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EC" id="1.13.11.-" evidence="2"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07108.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008641770.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0222"/>
    <dbReference type="EnsemblBacteria" id="ABF07108">
        <property type="protein sequence ID" value="ABF07108"/>
        <property type="gene ID" value="Rmet_0222"/>
    </dbReference>
    <dbReference type="GeneID" id="60822947"/>
    <dbReference type="KEGG" id="rme:Rmet_0222"/>
    <dbReference type="eggNOG" id="COG2070">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038732_3_0_4"/>
    <dbReference type="OMA" id="HGHEILA"/>
    <dbReference type="OrthoDB" id="275153at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051213">
        <property type="term" value="F:dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018580">
        <property type="term" value="F:nitronate monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04730">
        <property type="entry name" value="NPD_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004136">
        <property type="entry name" value="NMO"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03060">
        <property type="entry name" value="NMO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NMO" evidence="1">
        <location>
            <begin position="16"/>
            <end position="310"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03060"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF07108.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="325" mass="34146" checksum="6FCFB95E420DB1CD" modified="2006-05-30" version="1">MPAAKLIPPVLQNLALPVIASPMFIVSYPELVLAQCKAGIVGSFPALNARPAELLDEWLTQIQAELANFKAANPGKPVGPIAVNQIVHASNARLEHDVKVCVEHKVPIFITSLRAPPKEMIDAVHSYGGIVLHDVINIRHAEKAIEAGVDGLILVAAGAGGHAGTLSPFALVGEVRKFFDGPIALSGSIATGEAVLAAQAMGADFAYVGTRFIASQEAHAALEYKQSITSSAASDIIYTNLFTGVHGNYIRESIVNSGLDPENLPVADKSKMDFSSGSSKAKAWKDIWGAGQGVGQIEDIPTAGEIVARMKSEYDAARARLGLVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="72" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LRX2</accession>
    <name>Q1LRX2_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">ABC-type transporter, periplasmic component: HAAT family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_0218</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07104.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515123.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0218"/>
    <dbReference type="EnsemblBacteria" id="ABF07104">
        <property type="protein sequence ID" value="ABF07104"/>
        <property type="gene ID" value="Rmet_0218"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0218"/>
    <dbReference type="eggNOG" id="COG0683">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_050369_0_0_4"/>
    <dbReference type="OMA" id="PDYVLLW"/>
    <dbReference type="OrthoDB" id="776281at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028081">
        <property type="entry name" value="Leu-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028082">
        <property type="entry name" value="Peripla_BP_I"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13458">
        <property type="entry name" value="Peripla_BP_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53822">
        <property type="entry name" value="SSF53822"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193673" evidence="1">
        <location>
            <begin position="31"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="domain" description="Peripla_BP_6" evidence="2">
        <location>
            <begin position="36"/>
            <end position="405"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF07104.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="445" mass="48093" checksum="BB4D28269ED13593" modified="2006-05-30" version="1">MTTVFRNVQRAALVVSVAAAMLVPALPALAQTNDQFVALPSYRVGPYGANGQSFYGGFVDYLNYVNLKEGGVNGVKLGWEECETEYNNAKGVECYERLKAKSSASKGTAYHTMSTGISYALVDKTAADKVPLVMMGYGRTDAVDGSVFPYAFPLVTTYQMQVSAIVKFLASKNGGSLAGKKIVYLYHDSAYGKEPIVALEAEAKLGKFNLVEIPVAHPGNEQGAQWLKIRQENPDYVIFWGWGVMNQTALKAAQKVGYSRDKMIGSWWAGSEEDTVPAGEAAKGYMSATWNVAGKNVPLIADIEKVVYGAGKGNMQDRNKVGSVLYNRGVSAAVVTVEAIRGAQAKFGKGKVMSGDEMRWAFENLNLTSARLQQVGATGLLPEIKTSCDNHEGSGKVKIQQWDGAKWVVVSDWIEGNKSLIHPLFKATAAQYAKEKGITPACMKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="95" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LS76</accession>
    <name>Q1LS76_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Adenosylmethionine-8-amino-7-oxononanoate aminotransferase</fullName>
            <ecNumber evidence="4">2.6.1.62</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">7,8-diamino-pelargonic acid aminotransferase</fullName>
            <shortName evidence="4">DAPA AT</shortName>
            <shortName evidence="4">DAPA aminotransferase</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="4">7,8-diaminononanoate synthase</fullName>
            <shortName evidence="4">DANS</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="4">Diaminopelargonic acid synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="4 7" type="primary">bioA</name>
        <name evidence="7" type="ordered locus">Rmet_0114</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Catalyzes the transfer of the alpha-amino group from S-adenosyl-L-methionine (SAM) to 7-keto-8-aminopelargonic acid (KAPA) to form 7,8-diaminopelargonic acid (DAPA). It is the only aminotransferase known to utilize SAM as an amino donor.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>(8S)-8-amino-7-oxononanoate + S-adenosyl-L-methionine = (7R,8S)-7,8-diammoniononanoate + S-adenosyl-4-methylsulfanyl-2-oxobutanoate</text>
            <dbReference type="Rhea" id="RHEA:16861"/>
            <dbReference type="ChEBI" id="CHEBI:16490"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:149468"/>
            <dbReference type="ChEBI" id="CHEBI:149469"/>
            <dbReference type="EC" id="2.6.1.62"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 4">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="4">Cofactor biosynthesis; biotin biosynthesis; 7,8-diaminononanoate from 8-amino-7-oxononanoate (SAM route): step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family. BioA subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.6.1.62" evidence="4"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07000.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0114"/>
    <dbReference type="EnsemblBacteria" id="ABF07000">
        <property type="protein sequence ID" value="ABF07000"/>
        <property type="gene ID" value="Rmet_0114"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0114"/>
    <dbReference type="eggNOG" id="COG0161">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016922_4_3_4"/>
    <dbReference type="OMA" id="VAVKMCL"/>
    <dbReference type="UniPathway" id="UPA00078">
        <property type="reaction ID" value="UER00160"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004015">
        <property type="term" value="F:adenosylmethionine-8-amino-7-oxononanoate transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009102">
        <property type="term" value="P:biotin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00610">
        <property type="entry name" value="OAT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00834">
        <property type="entry name" value="BioA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005814">
        <property type="entry name" value="Aminotrans_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005815">
        <property type="entry name" value="BioA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00202">
        <property type="entry name" value="Aminotran_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000521">
        <property type="entry name" value="Transaminase_4ab_Lys_Orn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="SSF53383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00508">
        <property type="entry name" value="bioA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="4" id="KW-0093">Biotin biosynthesis</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 4 5" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="4 7" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="region of interest" description="Pyridoxal phosphate binding" evidence="4">
        <location>
            <begin position="146"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="region of interest" description="Pyridoxal phosphate binding" evidence="4">
        <location>
            <begin position="357"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="86"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="179"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="285"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="322"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="356"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="444"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Participates in the substrate recognition with KAPA and in a stacking interaction with the adenine ring of SAM" evidence="4">
        <location>
            <position position="47"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="4">
        <location>
            <position position="322"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00834"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABF07000.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="488" mass="52863" checksum="7AF0F1FC306463E8" modified="2006-05-30" version="1">MSFPHPAARDARADTHRRVPLTHPVLDSGGGDTSLAARSQRAVWHPCTRLRPDASANEPPLAIVRGEGPWLIDAGDKRYFDAISSWWVNLFGHANPAINAAIGEQLQTLEHVMLAGCTHAPAVELAERLTALTGGALGNAFYASDGASAVEIALKMSFHYWRNTGLTAKREFICLRHGYHGETVGALAVTDVEIFRDAYDPLTRRAHVVMSPDARQAARSESAADVATRALAELEALLRERAGQIAALIVEPLVQGAAGMAMYDPSYLEGARALCDRYRVHLIADEIAVGCGRTGTFFAWEQARGGDAVPAHAWPDFLCLSKGITGGYLPLSLVLSRPEIQRAFVADDIARSFLHSHSYTGNPLACRAALATLDLFESEDVLARNRERAQWLGTAMAGIAADPRVSHVRQRGMIWAADIDPQAAGANFAGRFHLAARERGVLARPIGHTLYVMPPYVFDAELANWLGEQLLDTLQAVLDPAEEARHAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LS82</accession>
    <name>Q1LS82_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Acyl-CoA dehydrogenase, short-chain specific</fullName>
            <ecNumber evidence="8">1.3.8.1</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Rmet_0108</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the acyl-CoA dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.3.8.1" evidence="8"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF06994.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515028.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0108"/>
    <dbReference type="EnsemblBacteria" id="ABF06994">
        <property type="protein sequence ID" value="ABF06994"/>
        <property type="gene ID" value="Rmet_0108"/>
    </dbReference>
    <dbReference type="GeneID" id="60822830"/>
    <dbReference type="KEGG" id="rme:Rmet_0108"/>
    <dbReference type="eggNOG" id="COG1960">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018204_0_2_4"/>
    <dbReference type="OMA" id="RDVKLNQ"/>
    <dbReference type="OrthoDB" id="760677at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003995">
        <property type="term" value="F:acyl-CoA dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004085">
        <property type="term" value="F:butyryl-CoA dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.540.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.110.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006089">
        <property type="entry name" value="Acyl-CoA_DH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006091">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046373">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036250">
        <property type="entry name" value="AcylCo_DH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009075">
        <property type="entry name" value="AcylCo_DH/oxidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013786">
        <property type="entry name" value="AcylCoA_DH/ox_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037069">
        <property type="entry name" value="AcylCoA_DH/ox_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009100">
        <property type="entry name" value="AcylCoA_DH/oxidase_NM_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00441">
        <property type="entry name" value="Acyl-CoA_dh_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02770">
        <property type="entry name" value="Acyl-CoA_dh_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02771">
        <property type="entry name" value="Acyl-CoA_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47203">
        <property type="entry name" value="SSF47203"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56645">
        <property type="entry name" value="SSF56645"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00073">
        <property type="entry name" value="ACYL_COA_DH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0274">FAD</keyword>
    <keyword evidence="3" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Acyl-CoA_dh_N" evidence="7">
        <location>
            <begin position="4"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA_dh_M" evidence="6">
        <location>
            <begin position="120"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA_dh_1" evidence="5">
        <location>
            <begin position="227"/>
            <end position="375"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF02770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF02771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ABF06994.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="376" mass="40409" checksum="E6D818F955F8D6A2" modified="2006-05-30" version="1">MLLTPEQEMIRDAVRQFAQDVIAPQAARWDRDKTFPKDVHKELARLGAYGVAVPEALGGAGLDYLSLALILEEIAAGDGGTSTVISVNNCPVCSMLMAFASDAQKQQWLVPLAQGDMLGAFCLTEPHVGSDASALRTTATRDGDHYVLNGVKQFITSGKHADVAIVMAVTDKAAGKRGISAFLVPTSTPGYIVARLEEKLGQHSSDTAQIVFENCRIPAANMLGEEGAGYKMALSGLEGGRIGIASQSIGMARAAFEAALAYAKERESFGQPLFQHQAVQFRLADMATQIDVARQMVWHAASLKDAGRPCLKEAAMAKLFASEMAERVCSDAIQVFGGYGYVTDFPVERIYRDVRVCQIYEGTSDIQKILIARALG</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LS93</accession>
    <name>Q1LS93_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">SPOR domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Rmet_0097</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF06983.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515018.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0097"/>
    <dbReference type="EnsemblBacteria" id="ABF06983">
        <property type="protein sequence ID" value="ABF06983"/>
        <property type="gene ID" value="Rmet_0097"/>
    </dbReference>
    <dbReference type="GeneID" id="60822819"/>
    <dbReference type="KEGG" id="rme:Rmet_0097"/>
    <dbReference type="eggNOG" id="COG3087">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_076835_1_0_4"/>
    <dbReference type="OMA" id="PIPGWLW"/>
    <dbReference type="OrthoDB" id="1741144at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042834">
        <property type="term" value="F:peptidoglycan binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1070">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007730">
        <property type="entry name" value="SPOR-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036680">
        <property type="entry name" value="SPOR-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05036">
        <property type="entry name" value="SPOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110997">
        <property type="entry name" value="SSF110997"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51724">
        <property type="entry name" value="SPOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="21"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="domain" description="SPOR" evidence="3">
        <location>
            <begin position="165"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="49"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="69"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="124"/>
            <end position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51724"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF06983.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="246" mass="26200" checksum="B6091870772446C7" modified="2006-05-30" version="1">MQQIAKRAPRTVRHTKTQRGGTFLGLVLGLIVGLAIAVVVALYITKSPAPFQQKGGSSAPAPRPSEPGNVVSTLPSPTQQAQQPEEPSDPNRPLWSKTPAKPVGQPEQAQPGQPAPQAPVATTRPADAGTNSASSTVAGKTPEKPAARPAEKPVADPIAEIAQADANKVGYLLQVGAFRSQDDADRQKANLAMQGFEAKVTDRDVNGVKMYRVRLGPFNRIDDMNRARDRLQSAGFEASVIRFTKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-01-04" modified="2022-08-03" version="134" xmlns="http://uniprot.org/uniprot">
    <accession>Q5NUV0</accession>
    <name>Q5NUV0_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 16 19">Mercuric reductase</fullName>
            <ecNumber evidence="5 16 19">1.16.1.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15 16 19">Hg(II) reductase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19 21" type="primary">merA</name>
        <name evidence="21" type="ordered locus">Rmet_6183</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="21 22">
        <name>pMOL28</name>
    </geneLocation>
    <reference evidence="22" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="22">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="22">Plasmid pMOL28</plasmid>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2 16">Resistance to Hg(2+) in bacteria appears to be governed by a specialized system which includes mercuric reductase. MerA protein is responsible for volatilizing mercury as Hg(0).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 16 19">
            <text>H(+) + Hg + NADP(+) = Hg(2+) + NADPH</text>
            <dbReference type="Rhea" id="RHEA:23856"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16170"/>
            <dbReference type="ChEBI" id="CHEBI:16793"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.16.1.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="16 17 19">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
        <text evidence="16 17 19">Binds 1 FAD per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4 16 19">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 16 19">Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family.</text>
    </comment>
    <dbReference type="EC" id="1.16.1.1" evidence="5 16 19"/>
    <dbReference type="EMBL" id="CP000355">
        <property type="protein sequence ID" value="ABF13042.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_003158917.1">
        <property type="nucleotide sequence ID" value="NC_007972.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_161727.1">
        <property type="nucleotide sequence ID" value="NC_006525.1"/>
    </dbReference>
    <dbReference type="SMR" id="Q5NUV0"/>
    <dbReference type="EnsemblBacteria" id="ABF13042">
        <property type="protein sequence ID" value="ABF13042"/>
        <property type="gene ID" value="Rmet_6183"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6183"/>
    <dbReference type="HOGENOM" id="CLU_016755_1_1_4"/>
    <dbReference type="OMA" id="GTCINWG"/>
    <dbReference type="OrthoDB" id="267896at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid pMOL28"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016152">
        <property type="term" value="F:mercury (II) reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045340">
        <property type="term" value="F:mercury ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016668">
        <property type="term" value="F:oxidoreductase activity, acting on a sulfur group of donors, NAD(P) as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045454">
        <property type="term" value="P:cell redox homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050787">
        <property type="term" value="P:detoxification of mercury ion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00371">
        <property type="entry name" value="HMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.390.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023753">
        <property type="entry name" value="FAD/NAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016156">
        <property type="entry name" value="FAD/NAD-linked_Rdtase_dimer_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017969">
        <property type="entry name" value="Heavy-metal-associated_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006121">
        <property type="entry name" value="HMA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036163">
        <property type="entry name" value="HMA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021179">
        <property type="entry name" value="Mercury_reductase_MerA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001100">
        <property type="entry name" value="Pyr_nuc-diS_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004099">
        <property type="entry name" value="Pyr_nucl-diS_OxRdtase_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012999">
        <property type="entry name" value="Pyr_OxRdtase_I_AS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00403">
        <property type="entry name" value="HMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07992">
        <property type="entry name" value="Pyr_redox_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02852">
        <property type="entry name" value="Pyr_redox_dim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000350">
        <property type="entry name" value="Mercury_reductase_MerA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="SSF51905"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55008">
        <property type="entry name" value="SSF55008"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55424">
        <property type="entry name" value="SSF55424"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02053">
        <property type="entry name" value="MerA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01047">
        <property type="entry name" value="HMA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50846">
        <property type="entry name" value="HMA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00076">
        <property type="entry name" value="PYRIDINE_REDOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 16 17" id="KW-0274">FAD</keyword>
    <keyword evidence="8 16 19" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="7 16" id="KW-0475">Mercuric resistance</keyword>
    <keyword evidence="12 16 19" id="KW-0476">Mercury</keyword>
    <keyword evidence="9 16 19" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="17" id="KW-0520">NAD</keyword>
    <keyword evidence="11 16 19" id="KW-0521">NADP</keyword>
    <keyword evidence="17" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13 16 19" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="21" id="KW-0614">Plasmid</keyword>
    <keyword evidence="14" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HMA" evidence="20">
        <location>
            <begin position="1"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="145"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <begin position="277"/>
            <end position="284"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="362"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="403"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="disulfide bond" description="Redox-active" evidence="18">
        <location>
            <begin position="136"/>
            <end position="141"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000896"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00003340"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012661"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00014791"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00031725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRNR" id="PIRNR000350"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRSR" id="PIRSR000350-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PIRSR" id="PIRSR000350-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="RuleBase" id="RU361223"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="ABF13042.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="561" mass="58700" checksum="F83E8157B8003DD9" modified="2005-01-04" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2005-01-04" modified="2021-02-10" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>Q5NUV4</accession>
    <name>Q5NUV4_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_6225</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="1 2">
        <name>pMOL28</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid pMOL28</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000355">
        <property type="protein sequence ID" value="ABF13084.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011239956.1">
        <property type="nucleotide sequence ID" value="NC_006525.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_161695.1">
        <property type="nucleotide sequence ID" value="NC_006525.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF13084">
        <property type="protein sequence ID" value="ABF13084"/>
        <property type="gene ID" value="Rmet_6225"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6225"/>
    <dbReference type="HOGENOM" id="CLU_153227_0_0_4"/>
    <dbReference type="OMA" id="GMSIHGD"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid pMOL28"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007607">
        <property type="entry name" value="BacA/B"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04519">
        <property type="entry name" value="Bactofilin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF13084.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="139" mass="14482" checksum="489D41BB813744FF" modified="2005-01-04" version="1">MTMPSTPTFSVLRAGMSIHGDLVSEDGFTCLALVTGNVRASHGLLHIGRGGVVHGDVEGEHIIIDGTVEGDVKSRVSLKINGRVIGRIFYAGTIRLGENVTLEGSSITRVPAVGYVASPQKEDAPAIHIDDPEVPGTAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-01-04" modified="2022-01-19" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>Q5NV49</accession>
    <name>Q5NV49_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Channel-forming protein, conjugal transfer protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">traG</name>
        <name evidence="4" type="ordered locus">Rmet_6309</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4 5">
        <name>pMOL28</name>
    </geneLocation>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="5">Plasmid pMOL28</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000355">
        <property type="protein sequence ID" value="ABF13168.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011239900.1">
        <property type="nucleotide sequence ID" value="NC_006525.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_161632.1">
        <property type="nucleotide sequence ID" value="NC_006525.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF13168">
        <property type="protein sequence ID" value="ABF13168"/>
        <property type="gene ID" value="Rmet_6309"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6309"/>
    <dbReference type="HOGENOM" id="CLU_024857_0_0_4"/>
    <dbReference type="OMA" id="EWTQKHC"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid pMOL28"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022458">
        <property type="entry name" value="Conjugative_coupling_TraG/TraD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002789">
        <property type="entry name" value="HerA_central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032689">
        <property type="entry name" value="TraG-D_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01935">
        <property type="entry name" value="DUF87"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12696">
        <property type="entry name" value="TraG-D_C"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03743">
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        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0614">Plasmid</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="domain" description="DUF87" evidence="2">
        <location>
            <begin position="162"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="domain" description="TraG-D_C" evidence="3">
        <location>
            <begin position="473"/>
            <end position="599"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01935"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF12696"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF13168.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="622" mass="69406" checksum="9ED18ED8F60BE7AB" modified="2005-01-04" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2010-03-23" modified="2020-10-07" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>D3DXQ1</accession>
    <name>D3DXQ1_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_6456</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
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            <taxon>Proteobacteria</taxon>
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            <taxon>Cupriavidus</taxon>
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    </organism>
    <reference evidence="2" key="1">
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            <authorList>
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                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ADC45071.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_6456"/>
    <dbReference type="EnsemblBacteria" id="ADC45071">
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        <property type="gene ID" value="Rmet_6456"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6456"/>
    <dbReference type="HOGENOM" id="CLU_3426659_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADC45071.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="21" mass="2500" checksum="C05D0B176739E78C" modified="2010-03-23" version="1">MYRSNARDAALCVMNFLVRRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-03-23" modified="2020-10-07" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>D3DXV1</accession>
    <name>D3DXV1_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_6513</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ADC45121.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADC45121">
        <property type="protein sequence ID" value="ADC45121"/>
        <property type="gene ID" value="Rmet_6513"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6513"/>
    <dbReference type="HOGENOM" id="CLU_3416986_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADC45121.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="26" mass="2956" checksum="5AC971DA745CBA42" modified="2010-03-23" version="1">MLSDSFIFASVHEIQLRAILDSHGRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-03-23" modified="2021-06-02" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>D3DY41</accession>
    <name>D3DY41_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_6612</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ADC45211.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_6612"/>
    <dbReference type="EnsemblBacteria" id="ADC45211">
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        <property type="gene ID" value="Rmet_6612"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6612"/>
    <dbReference type="HOGENOM" id="CLU_2303682_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="68"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="68"/>
            <end position="87"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADC45211.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="100" mass="10911" checksum="50029A7AE6363587" modified="2010-03-23" version="1">MMPDDGDLKGSPFSFAGTRVTLAEMGTLWSVRTPLPRRRRGVGGVGVQALPAALSQFEALFPLTRPLSRKRERGANQGGKRTQKRVRTSQECVTVPCPES</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-03-23" modified="2020-10-07" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>D3DY89</accession>
    <name>D3DY89_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_6660</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
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            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ADC45259.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADC45259">
        <property type="protein sequence ID" value="ADC45259"/>
        <property type="gene ID" value="Rmet_6660"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6660"/>
    <dbReference type="HOGENOM" id="CLU_2737165_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADC45259.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="71" mass="7998" checksum="3ECDFD7A8BDA53FC" modified="2010-03-23" version="1">MNSKQRRKAYRKSPYQIETTWTFRGAQVIITRIASPTAVIVEFEGAGARTHVPTRALEPRGPNGSMFRPLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-03-23" modified="2020-10-07" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>D3DYE8</accession>
    <name>D3DYE8_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_6739</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ADC45318.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADC45318">
        <property type="protein sequence ID" value="ADC45318"/>
        <property type="gene ID" value="Rmet_6739"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6739"/>
    <dbReference type="HOGENOM" id="CLU_2702168_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADC45318.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="73" mass="8004" checksum="CBD175D6D784734C" modified="2010-03-23" version="1">MTWRMSSRSFVQTSLDSSLALSLLSTEATALPSWGPPKLGAEIDSASGLLLGEKRGERRWALRIPGGSQFRQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LA86</accession>
    <name>Q1LA86_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_6081</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="1 2">
        <name>pMOL30</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid pMOL30</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000354">
        <property type="protein sequence ID" value="ABF12940.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011514860.1">
        <property type="nucleotide sequence ID" value="NC_007971.2"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF12940">
        <property type="protein sequence ID" value="ABF12940"/>
        <property type="gene ID" value="Rmet_6081"/>
    </dbReference>
    <dbReference type="GeneID" id="60825040"/>
    <dbReference type="KEGG" id="rme:Rmet_6081"/>
    <dbReference type="HOGENOM" id="CLU_1204016_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid pMOL30"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF12940.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="230" mass="24287" checksum="9F417C2040DDD8A0" modified="2006-05-30" version="1">MTNSLIRPTVGEVYQLLQGVSGLLVHFSGAPKGAGKTDAERLWFPDDLQKVLDGKAQGGLSASVVMPGDRFGQHYASNAVGCVGVILGLHSPQSLRCADAADCGSWTDQTGSRMCDAPASLSIQELALTISNRRQGCYNEWVIADYIPLGILAMPPFEVRTGGSPSDLPGGGDLSPELAGDSPVEVPKFLDLASVRRVFPSQPLYTMTGEGIALVGPDDSTSIILHDQIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LAR5</accession>
    <name>Q1LAR5_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Ethanolamine ammonia-lyase large subunit</fullName>
            <shortName evidence="1">EAL large subunit</shortName>
            <ecNumber evidence="1">4.3.1.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1 2" type="primary">eutB</name>
        <name evidence="2" type="ordered locus">Rmet_5902</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the deamination of various vicinal amino-alcohols to oxo compounds. Allows this organism to utilize ethanolamine as the sole source of nitrogen and carbon in the presence of vitamin B12.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ethanolamine = acetaldehyde + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:15313"/>
            <dbReference type="ChEBI" id="CHEBI:15343"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:57603"/>
            <dbReference type="EC" id="4.3.1.7"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>adenosylcob(III)alamin</name>
            <dbReference type="ChEBI" id="CHEBI:18408"/>
        </cofactor>
        <text evidence="1">Binds between the large and small subunits.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amine and polyamine degradation; ethanolamine degradation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">The basic unit is a heterodimer which dimerizes to form tetramers. The heterotetramers trimerize; 6 large subunits form a core ring with 6 small subunits projecting outwards.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Bacterial microcompartment</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the EutB family.</text>
    </comment>
    <dbReference type="EC" id="4.3.1.7" evidence="1"/>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12761.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518109.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5902"/>
    <dbReference type="EnsemblBacteria" id="ABF12761">
        <property type="protein sequence ID" value="ABF12761"/>
        <property type="gene ID" value="Rmet_5902"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5902"/>
    <dbReference type="eggNOG" id="COG4303">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_048555_0_0_4"/>
    <dbReference type="OMA" id="EADQNDM"/>
    <dbReference type="OrthoDB" id="230269at2"/>
    <dbReference type="UniPathway" id="UPA00560"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008851">
        <property type="term" value="F:ethanolamine ammonia-lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006520">
        <property type="term" value="P:cellular amino acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.220.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.170.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00861">
        <property type="entry name" value="EutB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010628">
        <property type="entry name" value="EutB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044939">
        <property type="entry name" value="EutB_dom_2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044941">
        <property type="entry name" value="EutB_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39329">
        <property type="entry name" value="PTHR39329"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06751">
        <property type="entry name" value="EutB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF018788">
        <property type="entry name" value="EutB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-1283">Bacterial microcompartment</keyword>
    <keyword evidence="1" id="KW-0846">Cobalamin</keyword>
    <keyword evidence="1" id="KW-0170">Cobalt</keyword>
    <keyword evidence="1 2" id="KW-0456">Lyase</keyword>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Substrate binding" evidence="1">
        <location>
            <begin position="160"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="193"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="194"/>
        </location>
        <ligand>
            <name>adenosylcob(III)alamin</name>
            <dbReference type="ChEBI" id="CHEBI:18408"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="246"/>
        </location>
        <ligand>
            <name>adenosylcob(III)alamin</name>
            <dbReference type="ChEBI" id="CHEBI:18408"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="288"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="296"/>
        </location>
        <ligand>
            <name>adenosylcob(III)alamin</name>
            <dbReference type="ChEBI" id="CHEBI:18408"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="363"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="402"/>
        </location>
        <ligand>
            <name>adenosylcob(III)alamin</name>
            <dbReference type="ChEBI" id="CHEBI:18408"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00861"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF12761.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="460" mass="49413" checksum="5113D8EA69A4886A" modified="2006-05-30" version="1">MAFTHTVGVRRHVFDDLRTLLAKASPARSGDALAGIAAASEEERMAARMALADMPLAHCLAEPLVPYEADEVTRLIVDSHDAQAFAALASLTVGEFRDWLLRHDTDTATLARVAPGVTPEMAAAVSKLMRNQDLVAVARKCAVVTAFRNTIGLPGRLSVRLQPNHPTDDPRGIAASIIDGLLFGCGDATIGINPASDNLGAITTLLKLLDDIRIRYEIPTQSCVLTHVTNTLRVMELGAPVDLVFQSVAGSQAANAAFGISLALLEEARQAALSLRRGTVGHNVMYFETGQGSALSANAHHGVDQQTMEARAYAVARRFEPLLVNTVVGFIGPEYLYDGKQIIRAGLEDHFCGKLLGVPMGCDVCYTNHAEADQDDMDNLLTLFGVAGINFIMGVPGADDIMLNYQSTSFHDALYLREVLGLRPAPEFEAWLRKMGIVDAGGRLLEPGTRQALLTMADTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="99" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LAT8</accession>
    <name>Q1LAT8_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Transcriptional regulator, LysR family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Rmet_5879</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="5">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="5">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the LysR transcriptional regulatory family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12738.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518086.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5879"/>
    <dbReference type="EnsemblBacteria" id="ABF12738">
        <property type="protein sequence ID" value="ABF12738"/>
        <property type="gene ID" value="Rmet_5879"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5879"/>
    <dbReference type="eggNOG" id="COG0583">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039613_6_0_4"/>
    <dbReference type="OMA" id="FATDDYP"/>
    <dbReference type="OrthoDB" id="990666at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005119">
        <property type="entry name" value="LysR_subst-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000847">
        <property type="entry name" value="Tscrpt_reg_HTH_LysR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00126">
        <property type="entry name" value="HTH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03466">
        <property type="entry name" value="LysR_substrate"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00039">
        <property type="entry name" value="HTHLYSR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50931">
        <property type="entry name" value="HTH_LYSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0614">Plasmid</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="226"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="domain" description="HTH lysR-type" evidence="3">
        <location>
            <begin position="5"/>
            <end position="62"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF12738.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="299" mass="32837" checksum="B304A083128AE76D" modified="2006-05-30" version="1">MLSNLSVKHLRAFVALATHRNFTRAAQACHLSQSAFSALIQTLEEQAGCRLFERTTRHVDLSTDGRRFEEMARRLLGDFEAAFDDLRDHAERRKGRVAIAALPSLAGGDLPPLLASFHQQYPGIALELHDQLADGCIEFVRRGQADFALAPAPAQDADLRVEALVRDSFHLVCPADHPLAGKRRVTPQALAGLPFIQLSRTSSVRQHLDAALHPLKLNGVMEVEHLATVAALVEAGLGVSVVPALALFQFRREGLAIRPMQMPTLVRDICVVRLKDRGDSAAATAMLDCLRAHYRSGRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LAU2</accession>
    <name>Q1LAU2_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_5875</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12734.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518084.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5875"/>
    <dbReference type="EnsemblBacteria" id="ABF12734">
        <property type="protein sequence ID" value="ABF12734"/>
        <property type="gene ID" value="Rmet_5875"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5875"/>
    <dbReference type="eggNOG" id="COG3170">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG4313">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041778_0_0_4"/>
    <dbReference type="OrthoDB" id="1970761at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193019" evidence="2">
        <location>
            <begin position="27"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="72"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="120"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="120"/>
            <end position="156"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF12734.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="490" mass="52473" checksum="5AE51338CE41FCC8" modified="2006-05-30" version="1">MKKRSLQGIGLASCSTMCLLGGMAHAQTPPDDGPPAAAKLETLQKELTEQAKQLEAMKRALAEQEATIRELRSTVDSESLARKRGGQAGVGTGVTPSNMATNAAAAAAGASATQINSTAQQGVQAQQAPSDAPPQAVAPQNVQQGQAAQGDQQGESAAGPEQPVGRPPERDTRPPEIAPIFDQPGVLTPQGKIVIEPSFQYGYSSSDRAVLVGYTVIPALLIGLIDVREIKTSTYITSMAFRYGITKRLEIEAKVPYVHSSSDTVSREIFTGTAVDNAFNASGSGLGDVEATLRYQLNYGNEKMPYFIGWLRYKSNTGKDPFEVVTDCVTRCVSNTTGTGLPLGMPTGTGFQAIQPGVTWLLPTDPAVFFGTFSYLHNFARDNIYRTVLNGQQEFLGKIAPGDIFEFNFGMGLSLNEHASFSIGYDQSIIWPTKQNGQTVPGSVRITQGTLLVGYSYRFNNRYTLNLSVGAGLTRDTPDLTVTVRLPITF</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="55" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LB32</accession>
    <name>Q1LB32_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_5785</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12644.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5785"/>
    <dbReference type="EnsemblBacteria" id="ABF12644">
        <property type="protein sequence ID" value="ABF12644"/>
        <property type="gene ID" value="Rmet_5785"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5785"/>
    <dbReference type="HOGENOM" id="CLU_2957394_0_0_4"/>
    <dbReference type="OMA" id="NNSMKQY"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="59"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF12644.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="59" mass="6253" checksum="0C50D2D2FED17EB2" modified="2006-05-30" version="1">MKKTDLEKNKGLKINNSMKQYGANRAGSLPKGSRRAETGKTGLLGKLLGKAVPPEKAGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="63" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LB70</accession>
    <name>Q1LB70_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_5747</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12606.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008643981.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5747"/>
    <dbReference type="EnsemblBacteria" id="ABF12606">
        <property type="protein sequence ID" value="ABF12606"/>
        <property type="gene ID" value="Rmet_5747"/>
    </dbReference>
    <dbReference type="GeneID" id="60823494"/>
    <dbReference type="KEGG" id="rme:Rmet_5747"/>
    <dbReference type="eggNOG" id="ENOG5033A3X">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_129162_1_0_4"/>
    <dbReference type="OMA" id="EWYRHHG"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024446">
        <property type="entry name" value="PXPV"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12778">
        <property type="entry name" value="PXPV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193031" evidence="1">
        <location>
            <begin position="28"/>
            <end position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF12606.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="118" mass="12902" checksum="B84BC874EE9379F5" modified="2006-05-30" version="1">MNNKRPLYFSIFLAGAAALAASASAMARVDIGVSLGVPAPVVVAPAPVVVAPAPVVVAPPPPAYIAPPPPVVYRPGPPVYVAPAVVVGWHGDRYWDGRRWYDRREYHAWHHHDDYYRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LBJ3</accession>
    <name>Q1LBJ3_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Hemolysin III family protein, channel protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Rmet_5624</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="8">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="8">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the UPF0073 (Hly-III) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12483.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011520029.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5624"/>
    <dbReference type="EnsemblBacteria" id="ABF12483">
        <property type="protein sequence ID" value="ABF12483"/>
        <property type="gene ID" value="Rmet_5624"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5624"/>
    <dbReference type="eggNOG" id="COG1272">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_051078_1_1_4"/>
    <dbReference type="OMA" id="HEVFHSL"/>
    <dbReference type="OrthoDB" id="1659567at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019835">
        <property type="term" value="P:cytolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004254">
        <property type="entry name" value="AdipoR/HlyIII-related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005744">
        <property type="entry name" value="Hy-lIII"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20855">
        <property type="entry name" value="PTHR20855"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03006">
        <property type="entry name" value="HlyIII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01065">
        <property type="entry name" value="hlyIII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0614">Plasmid</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="39"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="73"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="98"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="126"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="154"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="185"/>
            <end position="204"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABF12483.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="205" mass="22516" checksum="4FAD25D60F8E1B19" modified="2006-05-30" version="1">MYRGERFNGYSHLAGAVLSAAGMAVLVTSSALHSDAWKVVSSVVYGTTLVLLYTISTVYHSVRGPAKDIFRRLDYCAIYLLIAGSYTPFALVTLRGPWGWTLFGVNWGLAVAGIIQELWIGHRTRIFSLAIYVIMGWLVLIAFGPLAAALPPVGLYWLVAGGAIYTAGIGFFLFDEKVRHFHGIWHLFVLGGSMCQFISILFYVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LBP4</accession>
    <name>Q1LBP4_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Transcriptional regulator, LysR family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_5573</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the LysR transcriptional regulatory family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12432.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519982.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5573"/>
    <dbReference type="EnsemblBacteria" id="ABF12432">
        <property type="protein sequence ID" value="ABF12432"/>
        <property type="gene ID" value="Rmet_5573"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5573"/>
    <dbReference type="eggNOG" id="COG0583">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039613_37_1_4"/>
    <dbReference type="OMA" id="ARYAHHV"/>
    <dbReference type="OrthoDB" id="846433at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005119">
        <property type="entry name" value="LysR_subst-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000847">
        <property type="entry name" value="Tscrpt_reg_HTH_LysR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00126">
        <property type="entry name" value="HTH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03466">
        <property type="entry name" value="LysR_substrate"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00039">
        <property type="entry name" value="HTHLYSR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50931">
        <property type="entry name" value="HTH_LYSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH lysR-type" evidence="2">
        <location>
            <begin position="6"/>
            <end position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF12432.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="301" mass="33275" checksum="23F43A936822FED5" modified="2006-05-30" version="1">MRQRLPSLSNLRAFDAAALHLNLRRAAADLYITESAVSRQIASLESLLGVQLFHRANQRITLTAAGVLYSRQVREMLRKLQRDTLDIMAHEGVGGTVELACVPTLAVEWLIPRLPGFYARHPQVIVNIRAQSDVFLFEGTPYDAAIHFGEAVYPGARVDRMFGEESVAVCHPDFFGGKKRVSVNDIARSPLLHLVTRSFGWKQWMEAAGIDDVNALRGARYDHHSMVISAARAGLGVGLVPRFLVDEYLTRGLLVTPVARSLRATSAYYLVVPDTQPMSDAMGHLREWLLEAAGEFVGVAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LBQ5</accession>
    <name>Q1LBQ5_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Histone-like nucleoid-structuring protein H-NS</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Rmet_5562</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="8">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="8">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Nucleoid</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the histone-like protein H-NS family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12421.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519969.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5562"/>
    <dbReference type="EnsemblBacteria" id="ABF12421">
        <property type="protein sequence ID" value="ABF12421"/>
        <property type="gene ID" value="Rmet_5562"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5562"/>
    <dbReference type="eggNOG" id="COG2916">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_117503_5_1_4"/>
    <dbReference type="OMA" id="HWIAGAK"/>
    <dbReference type="OrthoDB" id="1911889at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009295">
        <property type="term" value="C:nucleoid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030527">
        <property type="term" value="F:structural constituent of chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027444">
        <property type="entry name" value="H-NS_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001801">
        <property type="entry name" value="Histone_HNS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00816">
        <property type="entry name" value="Histone_HNS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00528">
        <property type="entry name" value="HNS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-0614">Plasmid</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HNS" evidence="6">
        <location>
            <begin position="63"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="47"/>
            <end position="90"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004453"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010610"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABF12421.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="104" mass="11458" checksum="6EE0FF772D74AE64" modified="2006-05-30" version="1">MATYKQLLAQKEALEAQLAEVRATEVAGVIEQIRTLMEQYGLSVEDIQGKRRRGRPAGGGVAKAAKEPLPPKYRDPKTGKTWSGRGRAPAWLGKNPNRFLVAEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LBR2</accession>
    <name>Q1LBR2_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Fumarate reductase/succinate dehydrogenase flavoprotein-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Rmet_5555</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="6">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="6">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12414.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5555"/>
    <dbReference type="EnsemblBacteria" id="ABF12414">
        <property type="protein sequence ID" value="ABF12414"/>
        <property type="gene ID" value="Rmet_5555"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5555"/>
    <dbReference type="eggNOG" id="COG1053">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011398_4_2_4"/>
    <dbReference type="OMA" id="THIPCWL"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008152">
        <property type="term" value="P:metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003953">
        <property type="entry name" value="FAD-binding_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027477">
        <property type="entry name" value="Succ_DH/fumarate_Rdtase_cat_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00890">
        <property type="entry name" value="FAD_binding_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="SSF51905"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56425">
        <property type="entry name" value="SSF56425"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-0614">Plasmid</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD_binding_2" evidence="4">
        <location>
            <begin position="1"/>
            <end position="523"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00890"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABF12414.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="555" mass="59312" checksum="C1C88FD7DAFD7D4D" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="55" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LC39</accession>
    <name>Q1LC39_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_5428</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12287.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519835.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF12287">
        <property type="protein sequence ID" value="ABF12287"/>
        <property type="gene ID" value="Rmet_5428"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5428"/>
    <dbReference type="HOGENOM" id="CLU_2000104_0_0_4"/>
    <dbReference type="OMA" id="CFIDAER"/>
    <dbReference type="OrthoDB" id="1800689at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="95"/>
            <end position="124"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF12287.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="124" mass="12724" checksum="0E0B528EE010104C" modified="2006-05-30" version="1">MTNTAAPSSFLCTLPGQGKGIAYHVAVSFADAAQPTGVRFTSFVGDGRRSFGVVTGAGSLQGEIAANSEALCRLAIGQAVRDHLYEKTKEGDTALDLGAEPWDGELRPVGAGAPTGGLRHRPLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LCA1</accession>
    <name>Q1LCA1_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Transporter, major facilitator superfamily (MFS)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Rmet_5366</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="8">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="8">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12225.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519772.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5366"/>
    <dbReference type="EnsemblBacteria" id="ABF12225">
        <property type="protein sequence ID" value="ABF12225"/>
        <property type="gene ID" value="Rmet_5366"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5366"/>
    <dbReference type="eggNOG" id="COG2814">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001265_61_2_4"/>
    <dbReference type="OMA" id="EFAPMGL"/>
    <dbReference type="OrthoDB" id="1384345at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="SSF103473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0614">Plasmid</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="44"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="71"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="105"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="130"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="158"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="201"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="237"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="268"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="293"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="325"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="355"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="domain" description="MFS" evidence="6">
        <location>
            <begin position="6"/>
            <end position="381"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABF12225.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="388" mass="39011" checksum="CD568791A12614DD" modified="2006-05-30" version="1">MKSNTALLALAIGAFGIGTTEFTPMGLLPVIAEGVNVSIPTAGMLITAYAIGVMVGAPVMTLLFSRFGKRTALMALMAIFTIGNLLSALAPGYGTLLASRLVTSLNHGAFFGLGSVVAASVVPKEKQASAVATMFMGLTIANIGGVPAATWIGQQVGWRLAFAGTAALGLVAIAALWLALPKGERGTRPNVRRELTVLTHPTVLLAMATTVLGAGAMFTLYTYVAPMLAKLTGASDNFVALGLVLIGVGFTIGNHLGGKLADRSLDGATTTFLTALAVIMLVLPFALQSHVGAAVGLLVWGAAAFAVVPPLQMRVMEAAAEAPGLASSINVGAFNLGNAVGAALGGGVISLGLGYAVVPVAGGLLAASGVLLVWLGRASKRAGQPVKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LCJ4</accession>
    <name>Q1LCJ4_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Extra-cytoplasmic solute receptor protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">bug</name>
        <name evidence="4" type="ordered locus">Rmet_5273</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="5">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="5">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Periplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the UPF0065 (bug) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12132.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519678.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5273"/>
    <dbReference type="EnsemblBacteria" id="ABF12132">
        <property type="protein sequence ID" value="ABF12132"/>
        <property type="gene ID" value="Rmet_5273"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5273"/>
    <dbReference type="eggNOG" id="COG3181">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045683_0_0_4"/>
    <dbReference type="OMA" id="LAMMVNP"/>
    <dbReference type="OrthoDB" id="447734at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042597">
        <property type="term" value="C:periplasmic space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005064">
        <property type="entry name" value="BUG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042100">
        <property type="entry name" value="Bug_dom1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42928">
        <property type="entry name" value="PTHR42928"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03401">
        <property type="entry name" value="TctC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017082">
        <property type="entry name" value="YflP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-0675">Receptor</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193063" evidence="3">
        <location>
            <begin position="23"/>
            <end position="322"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF12132.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="322" mass="33346" checksum="1BF1A6808EF6CC4E" modified="2006-05-30" version="1">MLKTARLAVALCAATLSVTAPAQTNWPTRAVTIIVPFTPGGGTDIGTRLVAQRLSQLWGQPVVVDNRPGAAGNVGLELTARAKPDGYTLVAGNVGTQSINPFLYKKLSYNPDGFVPVTMMAELPFALVVTPGLPANTPKELVALAKAKPGKLTFASSGTGGSPHLSGEIFKAATSTDMLHVPYKGGGAAMTDLMAGNVDMLFASILETTGHVKAGKLRALAVTGTVRSPSMPDVPTLAEAGIQNAESGSWVGLLAPAGTPKEIVDKIAASVKQVVVIPDVKKQLIEQGAIPVGNTPQQFAAIIAQDRKRYAKIIADNHLSAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-10-07" version="60" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LCU5</accession>
    <name>Q1LCU5_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_5172</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12031.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5172"/>
    <dbReference type="EnsemblBacteria" id="ABF12031">
        <property type="protein sequence ID" value="ABF12031"/>
        <property type="gene ID" value="Rmet_5172"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5172"/>
    <dbReference type="HOGENOM" id="CLU_2435877_0_0_4"/>
    <dbReference type="OMA" id="GMYIHVA"/>
    <dbReference type="OrthoDB" id="2144979at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF12031.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="90" mass="10579" checksum="E1579AA9D7C6FFD4" modified="2006-05-30" version="1">MPARMRWLCQGYRGMYIHVAAFEEPGEPIQTCAPGPKWRYRMAIRYTADRDDRIFFESFDEEDDYYTHAAAEQAALHYGRFAVDLIYGMA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="97" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LD16</accession>
    <name>Q1LD16_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Transcriptional regulator, LysR family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_5101</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the LysR transcriptional regulatory family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11960.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519506.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5101"/>
    <dbReference type="EnsemblBacteria" id="ABF11960">
        <property type="protein sequence ID" value="ABF11960"/>
        <property type="gene ID" value="Rmet_5101"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5101"/>
    <dbReference type="eggNOG" id="COG0583">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039613_39_3_4"/>
    <dbReference type="OMA" id="QDNDPGE"/>
    <dbReference type="OrthoDB" id="1591366at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd08417">
        <property type="entry name" value="PBP2_Nitroaromatics_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005119">
        <property type="entry name" value="LysR_subst-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000847">
        <property type="entry name" value="Tscrpt_reg_HTH_LysR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037402">
        <property type="entry name" value="YidZ_PBP2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00126">
        <property type="entry name" value="HTH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03466">
        <property type="entry name" value="LysR_substrate"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50931">
        <property type="entry name" value="HTH_LYSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH lysR-type" evidence="2">
        <location>
            <begin position="15"/>
            <end position="74"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF11960.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="315" mass="34658" checksum="CFA881F3DED053FC" modified="2006-05-30" version="1">MTDIRGISVVDFKGVDLNLLVTLDALLDERNVTHTAERLGLTQPAVSAQLARLRRIFDDPLLIPADTGRGMIASARALALLEPLHVALKDLESVVHRRPEFDPFVDTRQFVIAASDNATSTLGMSLLERLSIVAGTGVRVAFVHGRQAEIAAQLESGEVDLLIGSERMVPPTMRARKLLDETFVMAQRKSHPRGARRLTLDMYCKLRHVLVSTSGGSFFGFVDEQLQLLGRSRDVAVSVQAFTVVPDLLRRSDYVCTMPARLAARYADWLDAFALPFDVPGFSLSAAWHPRNHADPAIAWLRSTLAEVAGQLREG</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LDI5</accession>
    <name>Q1LDI5_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Glutathione S-transferase enzyme with thioredoxin-like domain protein</fullName>
            <ecNumber evidence="4">2.5.1.18</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">gst</name>
        <name evidence="4" type="ordered locus">Rmet_4929</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="5">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="5">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the GST superfamily.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.18" evidence="4"/>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11791.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519351.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4929"/>
    <dbReference type="EnsemblBacteria" id="ABF11791">
        <property type="protein sequence ID" value="ABF11791"/>
        <property type="gene ID" value="Rmet_4929"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4929"/>
    <dbReference type="eggNOG" id="COG0625">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011226_14_4_4"/>
    <dbReference type="OMA" id="FPITRKW"/>
    <dbReference type="OrthoDB" id="1560019at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004364">
        <property type="term" value="F:glutathione transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006749">
        <property type="term" value="P:glutathione metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010987">
        <property type="entry name" value="Glutathione-S-Trfase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036282">
        <property type="entry name" value="Glutathione-S-Trfase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040079">
        <property type="entry name" value="Glutathione_S-Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004045">
        <property type="entry name" value="Glutathione_S-Trfase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004046">
        <property type="entry name" value="GST_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00043">
        <property type="entry name" value="GST_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02798">
        <property type="entry name" value="GST_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00019">
        <property type="entry name" value="Glutathione_Transferase_(cytos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47616">
        <property type="entry name" value="SSF47616"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="SSF52833"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50405">
        <property type="entry name" value="GST_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50404">
        <property type="entry name" value="GST_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0614">Plasmid</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="GST N-terminal" evidence="2">
        <location>
            <begin position="18"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="domain" description="GST C-terminal" evidence="3">
        <location>
            <begin position="108"/>
            <end position="235"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU003494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF11791.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="235" mass="26702" checksum="7CD811D91D584BC7" modified="2006-05-30" version="1">MADLSAFPITKRWPAQHPDRLQLYSLNTPNGIKVSLMLEETGLPYEPHLVRFDKNDQMTPEFLSLNPNNKIPAILDPNGPGGKPLALWESGAILLYLAEKTGKFMSADPALRYETIQWVMFQMGGIGPMFGQVGFFNRFAGKDFEDKRPRDRYVSESRRLLNVLNERLKGRQWIMGDDYTIADMATFPWIRNLLGFYEAGDLVGIQDFPEVTRVLGAFVARPAVQRAVNIPKRPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-10-07" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LE05</accession>
    <name>Q1LE05_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_4759</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11621.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4759"/>
    <dbReference type="EnsemblBacteria" id="ABF11621">
        <property type="protein sequence ID" value="ABF11621"/>
        <property type="gene ID" value="Rmet_4759"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4759"/>
    <dbReference type="HOGENOM" id="CLU_2303683_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="35"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF11621.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="100" mass="11147" checksum="030DBB5970F7548B" modified="2006-05-30" version="1">MISENGKDTPTARNGRHRARMGSCEFRPSSFIRTLTVGPGLSPDLLTPRGERGRSRARDNAPLAPATYRRWGIAPRPEDVTMPPRETSQRPEDLSTTVLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="100" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LE15</accession>
    <name>Q1LE15_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Histone-like bacterial DNA-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">hupB</name>
        <name evidence="4" type="ordered locus">Rmet_4749</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="5">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="5">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the bacterial histone-like protein family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11611.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008651272.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4749"/>
    <dbReference type="EnsemblBacteria" id="ABF11611">
        <property type="protein sequence ID" value="ABF11611"/>
        <property type="gene ID" value="Rmet_4749"/>
    </dbReference>
    <dbReference type="GeneID" id="60821661"/>
    <dbReference type="KEGG" id="rme:Rmet_4749"/>
    <dbReference type="eggNOG" id="COG0776">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_105066_3_1_4"/>
    <dbReference type="OMA" id="ISQEKQC"/>
    <dbReference type="OrthoDB" id="1847472at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030527">
        <property type="term" value="F:structural constituent of chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.520.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000119">
        <property type="entry name" value="Hist_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020816">
        <property type="entry name" value="Histone-like_DNA-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010992">
        <property type="entry name" value="IHF-like_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33175">
        <property type="entry name" value="PTHR33175"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00216">
        <property type="entry name" value="Bac_DNA_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01727">
        <property type="entry name" value="DNABINDINGHU"/>
    </dbReference>
    <dbReference type="SMART" id="SM00411">
        <property type="entry name" value="BHL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47729">
        <property type="entry name" value="SSF47729"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00045">
        <property type="entry name" value="HISTONE_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0614">Plasmid</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003939"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF11611.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="93" mass="9549" checksum="64FDABD209E67A90" modified="2006-05-30" version="1">MNKGELVSAIAAEAELSNAAAERALNAALDSIKKAVAKGDSVTLVGFGSFSSGKRAARTGRNPRTGETIKIPAAKTVKFSAGQGFKDAVNKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="97" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LE60</accession>
    <name>Q1LE60_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Type II secretion system protein G</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_4704</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11566.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519137.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4704"/>
    <dbReference type="EnsemblBacteria" id="ABF11566">
        <property type="protein sequence ID" value="ABF11566"/>
        <property type="gene ID" value="Rmet_4704"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4704"/>
    <dbReference type="eggNOG" id="COG2165">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_091705_7_2_4"/>
    <dbReference type="OMA" id="ELIVVMM"/>
    <dbReference type="OrthoDB" id="2044924at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015627">
        <property type="term" value="C:type II protein secretion system complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015628">
        <property type="term" value="P:protein secretion by the type II secretion system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000983">
        <property type="entry name" value="Bac_GSPG_pilin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012902">
        <property type="entry name" value="N_methyl_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045584">
        <property type="entry name" value="Pilin-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07963">
        <property type="entry name" value="N_methyl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00813">
        <property type="entry name" value="BCTERIALGSPG"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54523">
        <property type="entry name" value="SSF54523"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02532">
        <property type="entry name" value="IV_pilin_GFxxxE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00409">
        <property type="entry name" value="PROKAR_NTER_METHYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="123"/>
            <end position="145"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF11566.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="145" mass="15739" checksum="9FFBD87EDBB302A6" modified="2006-05-30" version="1">MRFPAFTWRRGFTLIEIVVVMAILGLLLTIALPRYLHAIDRGKEQVQRQNLAVMRDAIDKFYGDNGRYPETLDELVTRRYLRNVPDDPVSGSRDWVVVASPDGEKPGVYDLAPASAPEAVKPAAATTTAAGGASAATAAAERPSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="106" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LEI5</accession>
    <name>Q1LEI5_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Oligopeptide/dipeptide ABC transporter, ATP-binding protein-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">dppD</name>
        <name evidence="10" type="ordered locus">Rmet_4579</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="11">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="11">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11441.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008648527.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4579"/>
    <dbReference type="EnsemblBacteria" id="ABF11441">
        <property type="protein sequence ID" value="ABF11441"/>
        <property type="gene ID" value="Rmet_4579"/>
    </dbReference>
    <dbReference type="GeneID" id="60825951"/>
    <dbReference type="KEGG" id="rme:Rmet_4579"/>
    <dbReference type="eggNOG" id="COG0444">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_23_4"/>
    <dbReference type="OMA" id="YEPAHPY"/>
    <dbReference type="OrthoDB" id="1101128at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015833">
        <property type="term" value="P:peptide transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013563">
        <property type="entry name" value="Oligopep_ABC_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08352">
        <property type="entry name" value="oligo_HPY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01727">
        <property type="entry name" value="oligo_HPY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 8 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-0614">Plasmid</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-1278">Translocase</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ABC transporter" evidence="9">
        <location>
            <begin position="6"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="42"/>
            <end position="49"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ABF11441.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="325" mass="35500" checksum="4AD3DC441AA1BD02" modified="2006-05-30" version="1">MTEPILQVRDLTTRFRTDRGVVTAVDRVSFDVNAGETLAIVGESGSGKSVTALSILGLIPQPPGEIASGEIRFEGKDLLRLKPAEMRAIRGNRIAMIFQEPMSSMNPALTVGKQIAEPIRIHEHRPWRDAYDEAVALLAQVQIPEPRSRVQAYPHQFSGGMRQRAMIAMALACKPKLIIADEPTTALDVTVQAQILDLLKTLAQKSGTALILITHDLGVVARYADRVAVMYGGRLVEMASAEALYRHPAHPYTRGLIACVPRLDGDTSQPLVPIDGQPPDLTALGQGCAFRPRCKLANETCDQAPPPLREISHQHLNACFLHDAY</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LEJ2</accession>
    <name>Q1LEJ2_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Putative GTP cyclohydrolase II</fullName>
            <ecNumber evidence="9">3.5.4.25</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">Rmet_4572</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="10">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="10">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; riboflavin biosynthesis.</text>
    </comment>
    <dbReference type="EC" id="3.5.4.25" evidence="9"/>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11434.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008648510.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4572"/>
    <dbReference type="EnsemblBacteria" id="ABF11434">
        <property type="protein sequence ID" value="ABF11434"/>
        <property type="gene ID" value="Rmet_4572"/>
    </dbReference>
    <dbReference type="GeneID" id="60825958"/>
    <dbReference type="KEGG" id="rme:Rmet_4572"/>
    <dbReference type="eggNOG" id="COG0807">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029639_1_0_4"/>
    <dbReference type="OMA" id="AHRNVIG"/>
    <dbReference type="OrthoDB" id="900513at2"/>
    <dbReference type="UniPathway" id="UPA00275"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003935">
        <property type="term" value="F:GTP cyclohydrolase II activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009231">
        <property type="term" value="P:riboflavin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00641">
        <property type="entry name" value="GTP_cyclohydro2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10990">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022163">
        <property type="entry name" value="GTP_CH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032677">
        <property type="entry name" value="GTP_cyclohydro_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000926">
        <property type="entry name" value="RibA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036144">
        <property type="entry name" value="RibA-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12471">
        <property type="entry name" value="GTP_CH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00925">
        <property type="entry name" value="GTP_cyclohydro2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF142695">
        <property type="entry name" value="SSF142695"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="4 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-0614">Plasmid</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0686">Riboflavin biosynthesis</keyword>
    <feature type="domain" description="GTP_CH_N" evidence="8">
        <location>
            <begin position="4"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="domain" description="GTP_cyclohydro2" evidence="7">
        <location>
            <begin position="227"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="70"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022619"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00925"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF12471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ABF11434.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="412" mass="45133" checksum="8CDB066086D8C172" modified="2006-05-30" version="1">MSDHIVLTSHTRRDKPAEPIHWGAPTAAERGPVIASLTDPAQRNVIGTHAGAYAIYRALAVAAGSLQRAHRPDLTDTSPAEAIGPHPQWGDPDRIVSLDPWGHLVSTVFADRIAAGVDIRPTIAITRAHINMPELMAAISAGRLKPDGDILEANGDVRVTKAAVDPVWYLPGIARRFNIKESVLRRSLFEQTGGMFPELVTRPDLKVFLPPIGGMTLYFFGDVTKLGQPDTRVACRVHDECNGSDVFGSDICTCRPYLAHGIEVCIEMAQQGGVGLVVYNRKEGRALGEVTKFLVYNARKRQVGGDRAETYFERTECVAGVQDMRFQELMPDVFHWLGIRRIDRWASMSNMKHGALTAQGIEVVEQVAIPESLIPADARVEIDAKVAAGYFSRYTPPDAKELAQAKGRGLKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="99" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LEJ5</accession>
    <name>Q1LEJ5_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Transcriptional regulator, TetR family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_4569</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11431.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4569"/>
    <dbReference type="EnsemblBacteria" id="ABF11431">
        <property type="protein sequence ID" value="ABF11431"/>
        <property type="gene ID" value="Rmet_4569"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4569"/>
    <dbReference type="eggNOG" id="COG1309">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069356_1_0_4"/>
    <dbReference type="OMA" id="WIDAGKM"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045892">
        <property type="term" value="P:negative regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001647">
        <property type="entry name" value="HTH_TetR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036271">
        <property type="entry name" value="Tet_transcr_reg_TetR-rel_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013573">
        <property type="entry name" value="Tscrpt_reg_YcdC_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08362">
        <property type="entry name" value="TetR_C_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00440">
        <property type="entry name" value="TetR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00455">
        <property type="entry name" value="HTHTETR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48498">
        <property type="entry name" value="SSF48498"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50977">
        <property type="entry name" value="HTH_TETR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH tetR-type" evidence="2">
        <location>
            <begin position="20"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="H-T-H motif" evidence="1">
        <location>
            <begin position="43"/>
            <end position="62"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF11431.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="221" mass="24451" checksum="DDB8E429DAD3EE25" modified="2006-05-30" version="1">MMGAPALAPPVRESGGRIRQENEAQILRAAEHVFARAGFAGATMADIALHAGMPKSNLHYYFRTKQALYRAVLAHTLQLWLSETDIISADHAPRVALEQYIRAKMRLSASHPDASRVFANELLHGAPEIGGVLREALRELVTRKAMVIRSWIEDGQMARVDPYHLFFTIWAATQTYADFDAQVCAVLGVSQLDTEDFERATEHLVALLLRGCGLDQEASLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LEJ8</accession>
    <name>Q1LEJ8_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Rmet_4566</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="5">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="5">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11428.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519016.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4566"/>
    <dbReference type="EnsemblBacteria" id="ABF11428">
        <property type="protein sequence ID" value="ABF11428"/>
        <property type="gene ID" value="Rmet_4566"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4566"/>
    <dbReference type="eggNOG" id="COG5361">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027269_1_1_4"/>
    <dbReference type="OMA" id="MLRAYWP"/>
    <dbReference type="OrthoDB" id="547506at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.600">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1610">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010621">
        <property type="entry name" value="DUF1214"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037049">
        <property type="entry name" value="DUF1214_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010679">
        <property type="entry name" value="DUF1254"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037050">
        <property type="entry name" value="DUF1254_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06742">
        <property type="entry name" value="DUF1214"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06863">
        <property type="entry name" value="DUF1254"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0614">Plasmid</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193464" evidence="1">
        <location>
            <begin position="23"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="domain" description="DUF1254" evidence="3">
        <location>
            <begin position="73"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="domain" description="DUF1214" evidence="2">
        <location>
            <begin position="346"/>
            <end position="454"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF06742"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF06863"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF11428.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="470" mass="51772" checksum="C5CBEAA939DE72F0" modified="2006-05-30" version="1">MNSSIRRLAGAVALAVFLSACSSTSERSVADSAVEQPPSDQAMKTLSAEVFTYAYPLVLMDVTREVATAKVPVNTFGHKRAFPDAAFTDVVSPNGDTLYSSAWLDLSKEPMVLSVPDTSGRYYLMPMLDAWTNVFASPGKRTTGTKRGVFAIVGPNWQGQLPKGVKEIRSPTDMAWLIGRIQTNGKQDYANVHRLQDQFRLVPLSAWRKGSRTAVQPVPRAAMIDTETPPAEQVAAMDAQAFFTRLAALLPANPPAADDTAMVEKMQRMGIKAGQPFKTTVLEPSTARAVQEGASAALTMIKANGRKSAAGNMGWRVQRDIGVYGTDYGRRAVVAMFGLGANLPEDAIYPTARADAMGRPFDGGSRYVLHFDKSQLPPVKAFWSLTMYNEKQAFVSNSMQRYAIGSRDRLRYNRDGSLDIYIQYERPDERKVSNWLPAPPDAFNLMLRAYWPDQVVLDGKWMPPAVQRVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LEM9</accession>
    <name>Q1LEM9_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">VWFA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_4532</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11397.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518990.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4532"/>
    <dbReference type="EnsemblBacteria" id="ABF11397">
        <property type="protein sequence ID" value="ABF11397"/>
        <property type="gene ID" value="Rmet_4532"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4532"/>
    <dbReference type="eggNOG" id="COG2304">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024570_0_1_4"/>
    <dbReference type="OMA" id="RYFRATD"/>
    <dbReference type="OrthoDB" id="736429at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01467">
        <property type="entry name" value="vWA_BatA_type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.410">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033881">
        <property type="entry name" value="vWA_BatA_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002035">
        <property type="entry name" value="VWF_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036465">
        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00092">
        <property type="entry name" value="VWA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00327">
        <property type="entry name" value="VWA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53300">
        <property type="entry name" value="SSF53300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50234">
        <property type="entry name" value="VWFA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="298"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="domain" description="VWFA" evidence="2">
        <location>
            <begin position="95"/>
            <end position="279"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF11397.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="334" mass="36587" checksum="7BDF265A7B7CE88D" modified="2006-05-30" version="1">MITFEYPWLFALLPLPLLLWWWLPPYREESASVRLPFFGEVASAAGLKPALGAHVPRRNLVQLLLGPIAWGLVVVALARPQYLEPPLQRTQPVRDLLLALDLSQSMDTRDFKTPSGVLEPRVDAVRQVVADFVARRTGDRIGLIVFGDAPYPLAPFTLDHALVRELLADMVPGMAGASTSLGDAIGLGIKMFDQSHAQEKVMILLTDGNDTASRMPPAQAADIAKTRGVVVHTVGIGDPATTGEQKVDLDALKHIASTTGGRYFFGADQTSLASIYATLDRVTPHREKTLTWQPRRELFMWPLGAAVLLMLCYQLAMALYARWLARAAALESTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="100" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LF54</accession>
    <name>Q1LF54_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Transcriptional regulator, HTH GntR</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Rmet_4357</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="6">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="6">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11222.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008652314.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4357"/>
    <dbReference type="EnsemblBacteria" id="ABF11222">
        <property type="protein sequence ID" value="ABF11222"/>
        <property type="gene ID" value="Rmet_4357"/>
    </dbReference>
    <dbReference type="GeneID" id="60823122"/>
    <dbReference type="KEGG" id="rme:Rmet_4357"/>
    <dbReference type="eggNOG" id="COG1802">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017584_5_1_4"/>
    <dbReference type="OMA" id="DEHKHIV"/>
    <dbReference type="OrthoDB" id="1911275at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07377">
        <property type="entry name" value="WHTH_GntR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.530">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011711">
        <property type="entry name" value="GntR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008920">
        <property type="entry name" value="TF_FadR/GntR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000524">
        <property type="entry name" value="Tscrpt_reg_HTH_GntR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07729">
        <property type="entry name" value="FCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00392">
        <property type="entry name" value="GntR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00035">
        <property type="entry name" value="HTHGNTR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00895">
        <property type="entry name" value="FCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00345">
        <property type="entry name" value="HTH_GNTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48008">
        <property type="entry name" value="SSF48008"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50949">
        <property type="entry name" value="HTH_GNTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0614">Plasmid</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH gntR-type" evidence="4">
        <location>
            <begin position="26"/>
            <end position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABF11222.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="240" mass="27300" checksum="96D6AEFCA859E039" modified="2006-05-30" version="1">MSAQSQTDVQPTSGLTLSLQPINAGASLRDQAYAMLRQAIADADIYQSRDEVRLDERVLSEALGVSRTPIREAMTLLEQEGFLRTVPRRGIYITRKTKREIVEMIQVWGALESMAARLATLHASDEEIGKLRHMFDSFRDATPAEHIEEYSDANIAFHQAIVQLSKSQIIMDSIKNIFVHVRAIRKMTISQSDRAARSIVDHLRIIEALEKRDTELAERLVRQHSLDLADYVEKHCDFLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-10-07" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LFH8</accession>
    <name>Q1LFH8_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_4231</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11098.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4231"/>
    <dbReference type="EnsemblBacteria" id="ABF11098">
        <property type="protein sequence ID" value="ABF11098"/>
        <property type="gene ID" value="Rmet_4231"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4231"/>
    <dbReference type="HOGENOM" id="CLU_2247855_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="79"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF11098.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="104" mass="12081" checksum="6663522B45A62928" modified="2006-05-30" version="1">MAVSLSLFFSNIARYWNRPKFDYKKRICVAMVRRVVKLAGHLTRQCGRASPLQEKGAPRKQPLMNPSQIMGKCRHLSNTKHSIDHSKTSNGFVENYFQNEDRKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LFY3</accession>
    <name>Q1LFY3_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Acyl-CoA dehydrogenase-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_4075</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10943.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518568.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4075"/>
    <dbReference type="EnsemblBacteria" id="ABF10943">
        <property type="protein sequence ID" value="ABF10943"/>
        <property type="gene ID" value="Rmet_4075"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4075"/>
    <dbReference type="eggNOG" id="COG1960">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018204_5_0_4"/>
    <dbReference type="OMA" id="GSERWWS"/>
    <dbReference type="OrthoDB" id="760677at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016627">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-CH group of donors"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.540.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036250">
        <property type="entry name" value="AcylCo_DH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009075">
        <property type="entry name" value="AcylCo_DH/oxidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037069">
        <property type="entry name" value="AcylCoA_DH/ox_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009100">
        <property type="entry name" value="AcylCoA_DH/oxidase_NM_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00441">
        <property type="entry name" value="Acyl-CoA_dh_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47203">
        <property type="entry name" value="SSF47203"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56645">
        <property type="entry name" value="SSF56645"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0274">FAD</keyword>
    <keyword evidence="1" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Acyl-CoA_dh_1" evidence="2">
        <location>
            <begin position="180"/>
            <end position="302"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF10943.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="331" mass="35546" checksum="7AC75B2E1F6D32A4" modified="2006-05-30" version="1">MYNEYSEALEVLLRDHCTPAVVRQIESGGSGADLWRLLDESGFADSLLPEAGLSLADVAPMLFVMGRHVLPVPLAQTMFARAVLRMAGVEVPEGREGPEGSIALADLDGAQRTHVPCGATATWFLVQDGAQAALVHRDAVALSPTGVRDDLTLSLGEIGMQRFPQRFPLPAGLLRSVGACLHAVQMAGAMSSVFDLTLQYANDRVQFGRNIGKFQAIQHQISVMAEQVAAVRMAAQIACAADGWQPDRLRAAIGKFNASDAVTAVTSIAHAVHGAIGVTAEYDLQLYTRRLHAWRLADGSERYWARELGEATCEADGLTVDLIREWSGEAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LG33</accession>
    <name>Q1LG33_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Putative transmembrane transporter of the major facilitator superfamily MFS</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Rmet_4025</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="8">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="8">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10893.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4025"/>
    <dbReference type="EnsemblBacteria" id="ABF10893">
        <property type="protein sequence ID" value="ABF10893"/>
        <property type="gene ID" value="Rmet_4025"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4025"/>
    <dbReference type="eggNOG" id="COG2814">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001265_61_2_4"/>
    <dbReference type="OMA" id="GSIVIHQ"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001958">
        <property type="entry name" value="Tet-R_TetA/multi-R_MdtG"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01035">
        <property type="entry name" value="TCRTETA"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="SSF103473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0614">Plasmid</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="40"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="66"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="99"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="124"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="155"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="197"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="230"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="258"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="285"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="325"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="352"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="domain" description="MFS" evidence="6">
        <location>
            <begin position="1"/>
            <end position="377"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABF10893.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="382" mass="39318" checksum="A3E9DA03CD3E9C2E" modified="2006-05-30" version="1">MLALGVGSFGIGTGEFVIMGLLPDAARDLAITIPEAGHLISAYALGVVIGAPLLAVLGARWPRRNLLIALMSVFALGNFASALAPGYLPMLAVRLLTGFPHGTYFGVAALVAASLVARERRAQAVGLVMLGLTVATLVGVPIAAGIGTWLGWRTAFVIVGAIGALTALLVWRWVPFVPADRHASPLRELSALARKQVWFTLGIGAIGFGGMFAVFSYVKPTMLEVAHMPASGIPIVLALFGVGMVAGNLAGAKLADKALMPTIGGVLLWSALVLGAFVYTAPHPWLASLNVMLVGTAVALGPPLQIRLMDVAGDAQTLAAALNHSAFNMANALGAWLGGVTIAAGLGWTSTGWVGMLLALAGMVVFGWSLMSASRRDITVTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LG69</accession>
    <name>Q1LG69_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">BLUF domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_3989</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10857.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518497.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3989"/>
    <dbReference type="EnsemblBacteria" id="ABF10857">
        <property type="protein sequence ID" value="ABF10857"/>
        <property type="gene ID" value="Rmet_3989"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3989"/>
    <dbReference type="eggNOG" id="COG3431">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_097099_1_1_4"/>
    <dbReference type="OMA" id="FGGWTMG"/>
    <dbReference type="OrthoDB" id="1977204at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009882">
        <property type="term" value="F:blue light photoreceptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036046">
        <property type="entry name" value="Acylphosphatase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007024">
        <property type="entry name" value="BLUF_domain"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04940">
        <property type="entry name" value="BLUF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01034">
        <property type="entry name" value="BLUF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54975">
        <property type="entry name" value="SSF54975"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50925">
        <property type="entry name" value="BLUF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BLUF" evidence="1">
        <location>
            <begin position="2"/>
            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50925"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF10857.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="147" mass="16041" checksum="104BAD2D9DD26EC1" modified="2006-05-30" version="1">MLVRLLYASRARQPLDADVLDAILDTSLEQNLRHGITGVLCHSNTVFLQALEGDREAVSRLFLNIGRDDRHHHVVLLSYEEIAERAFAGWSMGRVNAGRINAATLLKYSAQGELDPCRMPGAASLALLTELIAGASIIGRGGERGRH</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LGF3</accession>
    <name>Q1LGF3_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">UPF0229 protein Rmet_3905</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_3905</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0229 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10773.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518419.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3905"/>
    <dbReference type="EnsemblBacteria" id="ABF10773">
        <property type="protein sequence ID" value="ABF10773"/>
        <property type="gene ID" value="Rmet_3905"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3905"/>
    <dbReference type="eggNOG" id="COG2718">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_049702_0_0_4"/>
    <dbReference type="OMA" id="QYFAYIE"/>
    <dbReference type="OrthoDB" id="481720at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01232">
        <property type="entry name" value="UPF0229"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006698">
        <property type="entry name" value="UPF0229"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036465">
        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30510">
        <property type="entry name" value="PTHR30510"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04285">
        <property type="entry name" value="DUF444"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53300">
        <property type="entry name" value="SSF53300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="66"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="66"/>
            <end position="87"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01232"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF10773.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="423" mass="48821" checksum="C9BECC866CB5BA0E" modified="2006-05-30" version="1">MATVIDRRENGGNKSAVNQQRFIKRYREQIRQAVAKAVSGRKIMDIEQSGQVSIPVKDISEPIFHHGQGGRREWIHPGNKKFVRGDSFDRQQQGGGGGGSRASDSGEGEDDFVFTLSREDFLNFFFEDMALPDLAKRHLAKIAEVRKVRAGFSIDGTPSNLSILRTMRSSLGRRIALSSPYLKRLRELELEYNDEVEKDPYSEKAEALMERMRHLRAQVDRVPFIEKLDLRYNNRVLKKRPQAQAVMFCLMDVSGSMDESRKDLAKRFFMLLYLFLKRNYERIDVVFIRHHTVAKEVDEEDFFHSRESGGTVVSSALKLMVEVIHDRYPPSQWNIYCAQASDGDNWAGDSELCGRLLRESILPLVQYYAYVEVASEEPQNLWEEYLTVKGEFEHFAMQRIIGADEIYPVLHDLFQKRAAWPGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="95" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LGG5</accession>
    <name>Q1LGG5_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_3893</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the LysR transcriptional regulatory family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10761.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518408.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3893"/>
    <dbReference type="EnsemblBacteria" id="ABF10761">
        <property type="protein sequence ID" value="ABF10761"/>
        <property type="gene ID" value="Rmet_3893"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3893"/>
    <dbReference type="eggNOG" id="COG0583">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039613_16_1_4"/>
    <dbReference type="OMA" id="LHAHECI"/>
    <dbReference type="OrthoDB" id="1661500at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005119">
        <property type="entry name" value="LysR_subst-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000847">
        <property type="entry name" value="Tscrpt_reg_HTH_LysR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00126">
        <property type="entry name" value="HTH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03466">
        <property type="entry name" value="LysR_substrate"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00039">
        <property type="entry name" value="HTHLYSR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50931">
        <property type="entry name" value="HTH_LYSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH lysR-type" evidence="2">
        <location>
            <begin position="8"/>
            <end position="65"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF10761.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="317" mass="35161" checksum="3C615799009D2948" modified="2006-05-30" version="1">MHRHFDDVLLGSIELFCLAAEAGSFTAAANTAGVTPAAVSRSISRLEKRLGVRLFVRSTRSIRLTEGGRSYFEQCRGALNQLIEAERKVTGEQVQPSGTLRISAPTTYGHYRLLPLLPALRERFPLLRFEVQIGNRNIDFHEENFDVAIRFRAQPDSTMVSRHLEDAELVVVASPGYLKRAGRPQALEDLQQHECIQFDLPSSGRPIAWLFMDGGREREVLATESHYLCAEDVLAGVTLARSGAGLFQTYRFIVERDLADGTLVEVLQPFAGRSRPVSLMYPHGRLLPSRVRVFVDFLMEQAAALASARGAGKPARG</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LGS8</accession>
    <name>Q1LGS8_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Glyoxalase/bleomycin resistance protein/dioxygenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_3778</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10648.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518294.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3778"/>
    <dbReference type="EnsemblBacteria" id="ABF10648">
        <property type="protein sequence ID" value="ABF10648"/>
        <property type="gene ID" value="Rmet_3778"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3778"/>
    <dbReference type="eggNOG" id="COG0346">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_084417_0_0_4"/>
    <dbReference type="OMA" id="DIGNDDR"/>
    <dbReference type="OrthoDB" id="971992at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051213">
        <property type="term" value="F:dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.180.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029068">
        <property type="entry name" value="Glyas_Bleomycin-R_OHBP_Dase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004360">
        <property type="entry name" value="Glyas_Fos-R_dOase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037523">
        <property type="entry name" value="VOC"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00903">
        <property type="entry name" value="Glyoxalase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54593">
        <property type="entry name" value="SSF54593"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51819">
        <property type="entry name" value="VOC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="VOC" evidence="1">
        <location>
            <begin position="19"/>
            <end position="153"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF10648.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="292" mass="32986" checksum="C6F6C553BD1E1904" modified="2006-05-30" version="1">MNQSTQGAHVNVPVVALHGVHHTARPTWKLAETVHFYRDLLGLPLVHAISAKGWGPDNHADFLHFFFDSGNGSTIAFFYYIGTERPDWLTVREHYQDRATHTAWRVRDEAELLQWRQRVEAVGIPLRYQIRHEVIESIYFNDPNGYPIEITWQVRPFSDADKQDAAFTIDSAIELERAREKGAAFASIEPVWQRKAERIERAVPNGEKASVYVLDVPEFAPLIDVARKTAGYQTTAIGNGYVRIDGNPTLQFRRKELGFKPAVWYGALTGGLAGSIRQFDMDALVVAPGDTQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-04-07" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LGY7</accession>
    <name>Q1LGY7_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Nitroreductase family protein (FMN dependent)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_3717</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the nitroreductase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10589.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3717"/>
    <dbReference type="EnsemblBacteria" id="ABF10589">
        <property type="protein sequence ID" value="ABF10589"/>
        <property type="gene ID" value="Rmet_3717"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3717"/>
    <dbReference type="eggNOG" id="COG0778">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_070764_4_1_4"/>
    <dbReference type="OMA" id="PWHFVVA"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02149">
        <property type="entry name" value="NfsB-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.109.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033878">
        <property type="entry name" value="NfsB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029479">
        <property type="entry name" value="Nitroreductase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000415">
        <property type="entry name" value="Nitroreductase-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00881">
        <property type="entry name" value="Nitroreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55469">
        <property type="entry name" value="SSF55469"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nitroreductase" evidence="2">
        <location>
            <begin position="10"/>
            <end position="196"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF10589.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="220" mass="24172" checksum="43252CB96CD8E613" modified="2006-05-30" version="1">MKAMKLSEMIATRHTVKAYEPGRAIPQDVIDELLAVLHKSPSSVNSQPWHFVVASTPEARARVARAASGGYSANAPRIENASHVIALAVRADMDNAHLNKLLEAEERDGRFTAEGAKAGQDKGRRYFVDLHRFAFRDVQQWMANQVYLALGSLLTGAAVLGVDATPMEGFDRNILDAELGLRERGYTSLVLVSLGYGSEQDFNADLPKSRLGKEEVFTFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LH14</accession>
    <name>Q1LH14_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">CheW protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">cheW</name>
        <name evidence="2" type="ordered locus">Rmet_3690</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10562.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008641041.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3690"/>
    <dbReference type="EnsemblBacteria" id="ABF10562">
        <property type="protein sequence ID" value="ABF10562"/>
        <property type="gene ID" value="Rmet_3690"/>
    </dbReference>
    <dbReference type="GeneID" id="60826245"/>
    <dbReference type="KEGG" id="rme:Rmet_3690"/>
    <dbReference type="eggNOG" id="COG0835">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_048995_1_0_4"/>
    <dbReference type="OMA" id="ERTIDTC"/>
    <dbReference type="OrthoDB" id="1711729at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006935">
        <property type="term" value="P:chemotaxis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039315">
        <property type="entry name" value="CheW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036061">
        <property type="entry name" value="CheW-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002545">
        <property type="entry name" value="CheW-lke_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22617">
        <property type="entry name" value="PTHR22617"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01584">
        <property type="entry name" value="CheW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00260">
        <property type="entry name" value="CheW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50341">
        <property type="entry name" value="SSF50341"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50851">
        <property type="entry name" value="CHEW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CheW-like" evidence="1">
        <location>
            <begin position="16"/>
            <end position="156"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50851"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF10562.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="164" mass="17809" checksum="B6E8476CB1C4C143" modified="2006-05-30" version="1">MAGIGHIDTQGSEASGQEYLVFTLGTEEYGIDILKVQEIRSYETVTRIANAPDFLKGVTNLRGVIVPIVDLRLKFKLGNVRYDHQTVVIILNVAGRVVGIVVDGVSDVLTLTGDDIKPAPEFGVSVSNEHLTGLGSVEGRMLILIDIERMMTSPEMELIEAELA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="102" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LH28</accession>
    <name>Q1LH28_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 8">UDP-glucose 4-epimerase</fullName>
            <ecNumber evidence="5 8">5.1.3.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">Rmet_3676</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="11">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="11">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 8">
            <text>UDP-alpha-D-glucose = UDP-alpha-D-galactose</text>
            <dbReference type="Rhea" id="RHEA:22168"/>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
            <dbReference type="ChEBI" id="CHEBI:66914"/>
            <dbReference type="EC" id="5.1.3.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 8">
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3 8">Carbohydrate metabolism; galactose metabolism.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 8">Belongs to the NAD(P)-dependent epimerase/dehydratase family.</text>
    </comment>
    <dbReference type="EC" id="5.1.3.2" evidence="5 8"/>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10548.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518199.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3676"/>
    <dbReference type="EnsemblBacteria" id="ABF10548">
        <property type="protein sequence ID" value="ABF10548"/>
        <property type="gene ID" value="Rmet_3676"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3676"/>
    <dbReference type="eggNOG" id="COG1087">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007383_1_10_4"/>
    <dbReference type="OMA" id="KVMTGQY"/>
    <dbReference type="OrthoDB" id="1597849at2"/>
    <dbReference type="UniPathway" id="UPA00214"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003978">
        <property type="term" value="F:UDP-glucose 4-epimerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006012">
        <property type="term" value="P:galactose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd05247">
        <property type="entry name" value="UDP_G4E_1_SDR_e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016040">
        <property type="entry name" value="NAD(P)-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005886">
        <property type="entry name" value="UDP_G4E"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16363">
        <property type="entry name" value="GDP_Man_Dehyd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01179">
        <property type="entry name" value="galE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="8" id="KW-0413">Isomerase</keyword>
    <keyword evidence="7 8" id="KW-0520">NAD</keyword>
    <keyword evidence="10" id="KW-0614">Plasmid</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAD(P)-bd_dom" evidence="9">
        <location>
            <begin position="6"/>
            <end position="325"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000083"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001911"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007637"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00013189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00018569"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU366046"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF16363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ABF10548.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="339" mass="36735" checksum="3181BC3026531E63" modified="2006-05-30" version="1">MAETLLLTGATGYIASHTWVALHEAGYNVIGLDNLCNSSAEVVNRLAKITGTTPHFVEGDVRDRALLDRLLAEHKINGAIHFAALKAVGESVSKPLEYYGNNLEGLITLCSAMQSAGVRQLVFSSSATVYGNPHAMPILENFPLSATNPYGQTKLMGEQILRDLEISDPEWKIAFLRYFNPVGAHESGTIGEDPGGIPNNLMPYVAQVAAGRREKLSVYGGDYPTPDGTGVRDYIHVSDLADGHVAALAYLGKNSRGMTVNLGTGRGYSVLEVVKAYERASGRPIPYEIVARRPGDIAECYADPGIARTLLGWSARHDLDRMCEDSWRWQSLNPVGFTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LHD7</accession>
    <name>Q1LHD7_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">DNA processing protein (DprA/Smf type)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_3567</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the DprA/Smf family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10439.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517982.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3567"/>
    <dbReference type="EnsemblBacteria" id="ABF10439">
        <property type="protein sequence ID" value="ABF10439"/>
        <property type="gene ID" value="Rmet_3567"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3567"/>
    <dbReference type="eggNOG" id="COG0758">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029601_1_1_4"/>
    <dbReference type="OMA" id="SEMPFGW"/>
    <dbReference type="OrthoDB" id="1881437at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009294">
        <property type="term" value="P:DNA-mediated transformation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003488">
        <property type="entry name" value="DprA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041614">
        <property type="entry name" value="DprA_WH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43022">
        <property type="entry name" value="PTHR43022"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02481">
        <property type="entry name" value="DNA_processg_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17782">
        <property type="entry name" value="DprA_WH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00732">
        <property type="entry name" value="dprA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DprA_WH" evidence="2">
        <location>
            <begin position="323"/>
            <end position="365"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF17782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF10439.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="371" mass="38107" checksum="81F5EE683F6E4488" modified="2006-05-30" version="1">MTDSTRDPAEVAAWLRLTATPGVSALAGRRLLAKFGLPQTVLAQNLASLREVVDAKVACVLNAPLDAAMSALIDRTVAWLADARHAMVTMADAGYPAGLLDLADPPLLLYINGDPARLAGPALGMVGARKATTQGARDAQAFASAFSNAGWTVVSGLALGIDAAAHAGALEGPGGTVAVIGTGADIVYPGRHHALAHRIVDAGGAIVSEFALGTPGLPNHFPRRNRIIAALSRGVLVVEAAERSGSLITARLASEIGREVFAMPGSIHAELARGCHKLIRQGAKLVETPADVFEEFGDPGGVASVAPVPVPDSAPVPGDPFGAALAYDPVTFDALCERSGLAPHMAAAMLLELELGGTVERMAGNRYRRLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LHF2</accession>
    <name>Q1LHF2_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">4HBT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_3552</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10424.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008641008.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3552"/>
    <dbReference type="EnsemblBacteria" id="ABF10424">
        <property type="protein sequence ID" value="ABF10424"/>
        <property type="gene ID" value="Rmet_3552"/>
    </dbReference>
    <dbReference type="GeneID" id="60822656"/>
    <dbReference type="KEGG" id="rme:Rmet_3552"/>
    <dbReference type="eggNOG" id="COG2050">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_089876_4_1_4"/>
    <dbReference type="OMA" id="NFAPWVL"/>
    <dbReference type="OrthoDB" id="1814691at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029069">
        <property type="entry name" value="HotDog_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003736">
        <property type="entry name" value="PAAI_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006683">
        <property type="entry name" value="Thioestr_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03061">
        <property type="entry name" value="4HBT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54637">
        <property type="entry name" value="SSF54637"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00369">
        <property type="entry name" value="unchar_dom_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="4HBT" evidence="1">
        <location>
            <begin position="53"/>
            <end position="128"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF10424.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="138" mass="14688" checksum="6044C38EDFA2216C" modified="2006-05-30" version="1">MPESTFTLADIDATLERVLAPWVRELGLRAEAVDADGVTLRLPFSESLRHAGGVVCGQVLMSAADTAMIVAIANALGNFRPMTTVSLTTNFMRPVIDGDVLVRANVLRLGKTVVFGEIELTGKDGKLAVQATTTYALL</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LHU8</accession>
    <name>Q1LHU8_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Cation Diffusion Factor, metal efflux system component</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">fieF</name>
        <name evidence="8" type="ordered locus">Rmet_3406</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10278.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3406"/>
    <dbReference type="TCDB" id="2.A.4.7.9">
        <property type="family name" value="the cation diffusion facilitator (cdf) family"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF10278">
        <property type="protein sequence ID" value="ABF10278"/>
        <property type="gene ID" value="Rmet_3406"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3406"/>
    <dbReference type="eggNOG" id="COG0053">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013430_3_3_4"/>
    <dbReference type="OMA" id="YGHQRIE"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008324">
        <property type="term" value="F:cation transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1510.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1350">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002524">
        <property type="entry name" value="Cation_efflux"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027470">
        <property type="entry name" value="Cation_efflux_CTD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036837">
        <property type="entry name" value="Cation_efflux_CTD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027469">
        <property type="entry name" value="Cation_efflux_TMD_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01545">
        <property type="entry name" value="Cation_efflux"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16916">
        <property type="entry name" value="ZT_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160240">
        <property type="entry name" value="SSF160240"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161111">
        <property type="entry name" value="SSF161111"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01297">
        <property type="entry name" value="CDF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="51"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="117"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="153"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="domain" description="ZT_dimer" evidence="7">
        <location>
            <begin position="248"/>
            <end position="323"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF16916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ABF10278.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="337" mass="35923" checksum="001CBA2CB143752D" modified="2006-05-30" version="1">MHVLLTPISIPFSLNTMRDSSIADDSLAPEVAQHDEAARARHLAGRRSTMVSVYVNIALSIAQAVIGIIAGSQALVADALHSLSDLISDFVVLFAGHHSRKDADTDHPYGHQRFETAASLAIGALLLAVGVGMLWAAVGKIQHPNGVQPVQTIAIWMALGALVAKELLFRYMLRVAERIRSSMLVANAWHARSDAASSLVVALGVGGNLLGYHVLDPVAAIVVGLMVSRTGLKFGWDALSDLMDRAADEDTVAALRAAMLETPGVLGLHDLKTRKMGDMILVDVHLEIQADLTVEQGHAIATEAARRAMARNDVLNVMTHVDPVRVTGVSGESRRSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-10-07" version="53" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LHZ1</accession>
    <name>Q1LHZ1_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_3363</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10235.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3363"/>
    <dbReference type="EnsemblBacteria" id="ABF10235">
        <property type="protein sequence ID" value="ABF10235"/>
        <property type="gene ID" value="Rmet_3363"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3363"/>
    <dbReference type="eggNOG" id="ENOG50332W9">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_844332_0_0_4"/>
    <dbReference type="OMA" id="MERVPRC"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009492">
        <property type="entry name" value="TniQ"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06527">
        <property type="entry name" value="TniQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF10235.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="329" mass="36949" checksum="43D01FB5CB6744A1" modified="2006-05-30" version="1">MRVPIRVEPFPEESLHGYLTRVGEANAIRFDRLRFELLGRKPKPSYALLGAFAESCRILPDEALRLGGFWKSDEGGTLYSMAGRLMLPERMLAPSMERVPRCKMCVRENGYQKSIWACTLLTTCHVHGVKLYNACESCGRPVGNDPWVKRTPCVCKSKGPIVAEASDGEIAIARLVEDGQLKAVPATLATSLGYLALEPVHRRLHTLHFLSDVFGRQARALISEGRVSSCPSRAYIAQQLLFVLSDWPAGFNDVFAKMSILDRQQNTVAAMRLWRLFRYYISDELWACSPFVRSAFEAAFNANFCKTLRRSSRLQIQESLDLVPSRDAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LI24</accession>
    <name>Q1LI24_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Cytochrome c, class I (C553-like)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Rmet_3330</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10202.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008649187.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3330"/>
    <dbReference type="EnsemblBacteria" id="ABF10202">
        <property type="protein sequence ID" value="ABF10202"/>
        <property type="gene ID" value="Rmet_3330"/>
    </dbReference>
    <dbReference type="GeneID" id="60826587"/>
    <dbReference type="KEGG" id="rme:Rmet_3330"/>
    <dbReference type="eggNOG" id="COG2863">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_128253_1_2_4"/>
    <dbReference type="OrthoDB" id="1939800at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.760.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009056">
        <property type="entry name" value="Cyt_c-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036909">
        <property type="entry name" value="Cyt_c-like_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00034">
        <property type="entry name" value="Cytochrom_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46626">
        <property type="entry name" value="SSF46626"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51007">
        <property type="entry name" value="CYTC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 4" id="KW-0349">Heme</keyword>
    <keyword evidence="3 4" id="KW-0408">Iron</keyword>
    <keyword evidence="2 4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193138" evidence="5">
        <location>
            <begin position="25"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="domain" description="Cytochrome c" evidence="6">
        <location>
            <begin position="9"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABF10202.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="109" mass="11949" checksum="3014D2CD2C2E2AE1" modified="2006-05-30" version="1">MTDFRLNRSAVTLALVLFSVGVHAETPKLDEKVKAILPLCASCHGIDGISSVGLYPNLSGQKPEYLAKQLHDFKAGRRNDPVMRPMAEPLDDAAIDALSRYFSKVKPPK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-12-12" modified="2022-08-03" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LI41</accession>
    <name>RS19_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">30S ribosomal protein S19</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpsS</name>
        <name type="ordered locus">Rmet_3313</name>
    </gene>
    <organism>
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Protein S19 forms a complex with S13 that binds strongly to the 16S ribosomal RNA.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uS19 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10185.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008642928.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q1LI41"/>
    <dbReference type="SMR" id="Q1LI41"/>
    <dbReference type="STRING" id="266264.Rmet_3313"/>
    <dbReference type="EnsemblBacteria" id="ABF10185">
        <property type="protein sequence ID" value="ABF10185"/>
        <property type="gene ID" value="Rmet_3313"/>
    </dbReference>
    <dbReference type="GeneID" id="60826604"/>
    <dbReference type="KEGG" id="rme:Rmet_3313"/>
    <dbReference type="eggNOG" id="COG0185">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_144911_0_1_4"/>
    <dbReference type="OMA" id="FPEMVGH"/>
    <dbReference type="OrthoDB" id="1893906at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015935">
        <property type="term" value="C:small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.860.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00531">
        <property type="entry name" value="Ribosomal_S19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020934">
        <property type="entry name" value="Ribosomal_S15/S19_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002222">
        <property type="entry name" value="Ribosomal_S19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005732">
        <property type="entry name" value="Ribosomal_S19_bac-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023575">
        <property type="entry name" value="Ribosomal_S19_S15_SF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11880">
        <property type="entry name" value="PTHR11880"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00203">
        <property type="entry name" value="Ribosomal_S19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002144">
        <property type="entry name" value="Ribosomal_S19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00975">
        <property type="entry name" value="RIBOSOMALS19"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54570">
        <property type="entry name" value="SSF54570"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01050">
        <property type="entry name" value="rpsS_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00323">
        <property type="entry name" value="RIBOSOMAL_S19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <feature type="chain" id="PRO_0000265409" description="30S ribosomal protein S19">
        <location>
            <begin position="1"/>
            <end position="91"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00531"/>
        </source>
    </evidence>
    <sequence length="91" mass="10080" checksum="9D13692BB58C2A41" modified="2006-05-30" version="1">MTRSAKKGPFCDGHLLKKVEAAVGGKDKKPIKTWSRRSTILPDFIGLTIAVHNGRQHVPVYVTENMVGHKLGEFAITRTFKGHAADKKAKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="65" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LID1</accession>
    <name>Q1LID1_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative metal dependent hydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">ygjP</name>
        <name evidence="2" type="ordered locus">Rmet_3223</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10095.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517706.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3223"/>
    <dbReference type="EnsemblBacteria" id="ABF10095">
        <property type="protein sequence ID" value="ABF10095"/>
        <property type="gene ID" value="Rmet_3223"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3223"/>
    <dbReference type="eggNOG" id="COG1451">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_101303_0_0_4"/>
    <dbReference type="OMA" id="LCCYMEP"/>
    <dbReference type="OrthoDB" id="1301886at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002725">
        <property type="entry name" value="YgjP-like_metallopeptidase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01863">
        <property type="entry name" value="DUF45"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF45" evidence="1">
        <location>
            <begin position="90"/>
            <end position="146"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01863"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF10095.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="165" mass="19358" checksum="AE1121BA68AED8BF" modified="2006-05-30" version="1">MRFLGGYPPDVLAQVRALIDADRLGEHIARRYPDRHTIQTDRALYDYTSEIKQEYLRSAPPLHKVRYDARLDTAQHALGLHTAISRVQGGKLKAKKEIRVASLFKEAPPEFLRMIVVHELAHLKETDHDKAFYRLCEYMEPRYHQLEFDTRLFLAWREMEKRGGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="96" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LIR6</accession>
    <name>Q1LIR6_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 12">Ribonucleoside-diphosphate reductase</fullName>
            <ecNumber evidence="4 12">1.17.4.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">nrdA</name>
        <name evidence="14" type="ordered locus">Rmet_3088</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 12">
            <text>[thioredoxin]-disulfide + a 2'-deoxyribonucleoside 5'-diphosphate + H2O = [thioredoxin]-dithiol + a ribonucleoside 5'-diphosphate</text>
            <dbReference type="Rhea" id="RHEA:23252"/>
            <dbReference type="Rhea" id="RHEA-COMP:10698"/>
            <dbReference type="Rhea" id="RHEA-COMP:10700"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:50058"/>
            <dbReference type="ChEBI" id="CHEBI:57930"/>
            <dbReference type="ChEBI" id="CHEBI:73316"/>
            <dbReference type="EC" id="1.17.4.1"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 12">Genetic information processing; DNA replication.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 12">Belongs to the ribonucleoside diphosphate reductase large chain family.</text>
    </comment>
    <dbReference type="EC" id="1.17.4.1" evidence="4 12"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09960.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517588.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3088"/>
    <dbReference type="EnsemblBacteria" id="ABF09960">
        <property type="protein sequence ID" value="ABF09960"/>
        <property type="gene ID" value="Rmet_3088"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3088"/>
    <dbReference type="eggNOG" id="COG0209">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000404_3_0_4"/>
    <dbReference type="OMA" id="RGSIQNI"/>
    <dbReference type="OrthoDB" id="357568at2"/>
    <dbReference type="UniPathway" id="UPA00326"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004748">
        <property type="term" value="F:ribonucleoside-diphosphate reductase activity, thioredoxin disulfide as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005144">
        <property type="entry name" value="ATP-cone_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013346">
        <property type="entry name" value="NrdE_NrdA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000788">
        <property type="entry name" value="RNR_lg_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013509">
        <property type="entry name" value="RNR_lsu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008926">
        <property type="entry name" value="RNR_R1-su_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039718">
        <property type="entry name" value="Rrm1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11573">
        <property type="entry name" value="PTHR11573"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03477">
        <property type="entry name" value="ATP-cone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02867">
        <property type="entry name" value="Ribonuc_red_lgC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00317">
        <property type="entry name" value="Ribonuc_red_lgN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01183">
        <property type="entry name" value="RIBORDTASEM1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48168">
        <property type="entry name" value="SSF48168"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02506">
        <property type="entry name" value="NrdE_NrdA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51161">
        <property type="entry name" value="ATP_CONE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00089">
        <property type="entry name" value="RIBORED_LARGE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0021">Allosteric enzyme</keyword>
    <keyword evidence="8 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6 12" id="KW-0235">DNA replication</keyword>
    <keyword evidence="7 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9 12 14" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ATP-cone" evidence="13">
        <location>
            <begin position="38"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="domain" description="ATP-cone" evidence="13">
        <location>
            <begin position="156"/>
            <end position="245"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU003410"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ABF09960.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="979" mass="108463" checksum="5E6E776FC37BA6A8" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-09-02" modified="2022-08-03" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LIS6</accession>
    <name>Y3078_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">PKHD-type hydroxylase Rmet_3078</fullName>
            <ecNumber evidence="1">1.14.11.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">Rmet_3078</name>
    </gene>
    <organism>
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
        <text evidence="1">Binds 1 Fe(2+) ion per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>L-ascorbate</name>
            <dbReference type="ChEBI" id="CHEBI:38290"/>
        </cofactor>
    </comment>
    <dbReference type="EC" id="1.14.11.-" evidence="1"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09950.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517579.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q1LIS6"/>
    <dbReference type="SMR" id="Q1LIS6"/>
    <dbReference type="STRING" id="266264.Rmet_3078"/>
    <dbReference type="EnsemblBacteria" id="ABF09950">
        <property type="protein sequence ID" value="ABF09950"/>
        <property type="gene ID" value="Rmet_3078"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3078"/>
    <dbReference type="eggNOG" id="COG3128">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_106663_0_0_4"/>
    <dbReference type="OMA" id="VGCYHNL"/>
    <dbReference type="OrthoDB" id="1139586at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016706">
        <property type="term" value="F:2-oxoglutarate-dependent dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031418">
        <property type="term" value="F:L-ascorbic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00657">
        <property type="entry name" value="Hydroxyl_YbiX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005123">
        <property type="entry name" value="Oxoglu/Fe-dep_dioxygenase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041097">
        <property type="entry name" value="PKHD_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023550">
        <property type="entry name" value="PKHD_hydroxylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006620">
        <property type="entry name" value="Pro_4_hyd_alph"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044862">
        <property type="entry name" value="Pro_4_hyd_alph_FE2OG_OXY"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR41536">
        <property type="entry name" value="PTHR41536"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13640">
        <property type="entry name" value="2OG-FeII_Oxy_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18331">
        <property type="entry name" value="PKHD_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00702">
        <property type="entry name" value="P4Hc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51471">
        <property type="entry name" value="FE2OG_OXY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0223">Dioxygenase</keyword>
    <keyword id="KW-0408">Iron</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0560">Oxidoreductase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0847">Vitamin C</keyword>
    <feature type="chain" id="PRO_0000346516" description="PKHD-type hydroxylase Rmet_3078">
        <location>
            <begin position="1"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="domain" description="Fe2OG dioxygenase" evidence="1">
        <location>
            <begin position="80"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="98"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="100"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="161"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="171"/>
        </location>
        <ligand>
            <name>2-oxoglutarate</name>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00657"/>
        </source>
    </evidence>
    <sequence length="228" mass="25463" checksum="8F6921D9BBCAA275" modified="2006-05-30" version="1">MLVRIPQVLNAEQLAMLREQLDHAGDAWVDGRVTAGYSGAPVKFNQQIDERSEAAAQCQHLVLSALERNPLFISAVLPNIVYPPMFNRYSEGMTFGLHVDGGVRLHPHNGRKLRTDVSATLFLSDPASYDGGELQIEDTYGVHSVKLAAGDMVVYPSTSLHQVKPITRGVRVGCFFWIQSLIRDDGQRALLFDMDNAIQTLNQTNADERARRTLVGCYHNLLRQWSDT</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="70" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LIU0</accession>
    <name>Q1LIU0_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 3">Biotin carboxyl carrier protein of acetyl-CoA carboxylase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">accB</name>
        <name evidence="5" type="ordered locus">Rmet_3064</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 3">This protein is a component of the acetyl coenzyme A carboxylase complex; first, biotin carboxylase catalyzes the carboxylation of the carrier protein and then the transcarboxylase transfers the carboxyl group to form malonyl-CoA.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3">Lipid metabolism; fatty acid biosynthesis.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09936.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517564.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3064"/>
    <dbReference type="EnsemblBacteria" id="ABF09936">
        <property type="protein sequence ID" value="ABF09936"/>
        <property type="gene ID" value="Rmet_3064"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3064"/>
    <dbReference type="eggNOG" id="COG0511">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016733_3_1_4"/>
    <dbReference type="OMA" id="IKSPIIG"/>
    <dbReference type="OrthoDB" id="1938042at2"/>
    <dbReference type="UniPathway" id="UPA00094"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009317">
        <property type="term" value="C:acetyl-CoA carboxylase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003989">
        <property type="term" value="F:acetyl-CoA carboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006633">
        <property type="term" value="P:fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001249">
        <property type="entry name" value="AcCoA_biotinCC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000089">
        <property type="entry name" value="Biotin_lipoyl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011053">
        <property type="entry name" value="Single_hybrid_motif"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00364">
        <property type="entry name" value="Biotin_lipoyl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01071">
        <property type="entry name" value="ACOABIOTINCC"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51230">
        <property type="entry name" value="SSF51230"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00531">
        <property type="entry name" value="BCCP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50968">
        <property type="entry name" value="BIOTINYL_LIPOYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0092">Biotin</keyword>
    <keyword evidence="3" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="3" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="3" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="3" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Lipoyl-binding" evidence="4">
        <location>
            <begin position="77"/>
            <end position="153"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00017562"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU364072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABF09936.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="153" mass="15976" checksum="456DC6374B1FA297" modified="2006-05-30" version="1">MDLRKLKTLIDLVAESGISELEVTEGEGKVRIVKQPPQVMAAPMAMPQYQQLPAAPAVNVAGAPAAAAAEAPQVPAGHVVTSPMVGTFYRAPSPGAAPFVNVGDTVKEGQTVCIIEAMKLLNEIECDKAGVIKEILVENGQAVEYGQPLFVIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LIV5</accession>
    <name>Q1LIV5_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Competence/damage-induced protein, CinA-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_3049</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09921.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517552.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3049"/>
    <dbReference type="EnsemblBacteria" id="ABF09921">
        <property type="protein sequence ID" value="ABF09921"/>
        <property type="gene ID" value="Rmet_3049"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3049"/>
    <dbReference type="eggNOG" id="COG1546">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030805_1_1_4"/>
    <dbReference type="OMA" id="IYICVIV"/>
    <dbReference type="OrthoDB" id="1834406at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.950.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036653">
        <property type="entry name" value="CinA-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008136">
        <property type="entry name" value="CinA_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02464">
        <property type="entry name" value="CinA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF142433">
        <property type="entry name" value="SSF142433"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00199">
        <property type="entry name" value="PncC_domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CinA" evidence="1">
        <location>
            <begin position="9"/>
            <end position="160"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF09921.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="165" mass="17289" checksum="E341D5C5D2D0B654" modified="2006-05-30" version="1">MSVSRLLDQLAIQAGAALSESSLMLATAESCTGGLVAAAITDVSGSSGWFERGFVTYSNEAKTTMVGVPAKLIRDHGAVSEEVARAMAEGALLNSRAQVSLAITGVAGPGGGTPEKPVGMVCFGWSNRITTHTETQRFKGDRAQIRRQAAEHAMRGLLELIRNEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="95" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LJ56</accession>
    <name>Q1LJ56_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Protein-export membrane protein SecF</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9 11" type="primary">secF</name>
        <name evidence="11" type="ordered locus">Rmet_2947</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Part of the Sec protein translocase complex. Interacts with the SecYEG preprotein conducting channel. SecDF uses the proton motive force (PMF) to complete protein translocation after the ATP-dependent function of SecA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Forms a complex with SecD. Part of the essential Sec protein translocation apparatus which comprises SecA, SecYEG and auxiliary proteins SecDF-YajC and YidC.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cell inner membrane</location>
            <topology evidence="9">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the SecD/SecF family. SecF subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09820.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517487.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2947"/>
    <dbReference type="EnsemblBacteria" id="ABF09820">
        <property type="protein sequence ID" value="ABF09820"/>
        <property type="gene ID" value="Rmet_2947"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2947"/>
    <dbReference type="eggNOG" id="COG0341">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_050012_1_0_4"/>
    <dbReference type="OMA" id="AFEWRMA"/>
    <dbReference type="OrthoDB" id="410730at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015450">
        <property type="term" value="F:protein-transporting ATPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0065002">
        <property type="term" value="P:intracellular protein transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006605">
        <property type="term" value="P:protein targeting"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043952">
        <property type="term" value="P:protein transport by the Sec complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01464_B">
        <property type="entry name" value="SecF_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022813">
        <property type="entry name" value="SecD/SecF_arch_bac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022645">
        <property type="entry name" value="SecD/SecF_bac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022646">
        <property type="entry name" value="SecD/SecF_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005665">
        <property type="entry name" value="SecF_bac"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30081">
        <property type="entry name" value="PTHR30081"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07549">
        <property type="entry name" value="Sec_GG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02355">
        <property type="entry name" value="SecD_SecF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01755">
        <property type="entry name" value="SECFTRNLCASE"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00916">
        <property type="entry name" value="2A0604s01"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00966">
        <property type="entry name" value="3a0501s07"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="3 9" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 9" id="KW-0653">Protein transport</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 9" id="KW-0811">Translocation</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 9" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="12"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="142"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="166"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="193"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="253"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="277"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="304"/>
            <end position="324"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ABF09820.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="324" mass="35971" checksum="42E33DDFE4AD60B3" modified="2006-05-30" version="1">MEFFRIKRDIPFMKHALIFNVVSFLTFAAAVFFLWHKGLHLSIEFTGGTVMEVNYQQAVDLEKVRGQVSKLGYSDVQVQNFGTSRDVMIRLPLQKGPDGKQVTSAQQSEQVMGALTAASPDVKLQRVEFVGPQVGKELATDGLLALLCVVAGIVIYLSFRFEWKFAVAGIIANLHDVVIILGFFAFFQWEFSLSVLAAILAVLGYSVNESVVIFDRIREAFRKYRKMTTHEVIDHAITSTMSRTIITHGSTEMMVLSMFFFGGPTLHYFALALTVGILFGIYSSVFVAAALAMWLGVKREDLVKGDKKGGDVTDRNDPNFGAQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="109" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LJB7</accession>
    <name>Q1LJB7_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Lipoprotein signal peptidase</fullName>
            <ecNumber evidence="6">3.4.23.36</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Prolipoprotein signal peptidase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">Signal peptidase II</fullName>
            <shortName evidence="6">SPase II</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6 10" type="primary">lspA</name>
        <name evidence="10" type="ordered locus">Rmet_2886</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6 7">This protein specifically catalyzes the removal of signal peptides from prolipoproteins.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6 7">
            <text>Release of signal peptides from bacterial membrane prolipoproteins. Hydrolyzes -Xaa-Yaa-Zaa-|-(S,diacylglyceryl)Cys-, in which Xaa is hydrophobic (preferably Leu), and Yaa (Ala or Ser) and Zaa (Gly or Ala) have small, neutral side chains.</text>
            <dbReference type="EC" id="3.4.23.36"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="6">Protein modification; lipoprotein biosynthesis (signal peptide cleavage).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cell inner membrane</location>
            <topology evidence="6">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 6 8">Belongs to the peptidase A8 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.23.36" evidence="6"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09759.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517439.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2886"/>
    <dbReference type="EnsemblBacteria" id="ABF09759">
        <property type="protein sequence ID" value="ABF09759"/>
        <property type="gene ID" value="Rmet_2886"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2886"/>
    <dbReference type="eggNOG" id="COG0597">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_083252_4_0_4"/>
    <dbReference type="OMA" id="NRWYFPA"/>
    <dbReference type="OrthoDB" id="1575081at2"/>
    <dbReference type="UniPathway" id="UPA00665"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004190">
        <property type="term" value="F:aspartic-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00161">
        <property type="entry name" value="LspA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001872">
        <property type="entry name" value="Peptidase_A8"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33695">
        <property type="entry name" value="PTHR33695"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01252">
        <property type="entry name" value="Peptidase_A8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00781">
        <property type="entry name" value="LIPOSIGPTASE"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00077">
        <property type="entry name" value="lspA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00855">
        <property type="entry name" value="SPASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6 7" id="KW-0064">Aspartyl protease</keyword>
    <keyword evidence="6" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="2 6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4 6 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 7" id="KW-0645">Protease</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="27"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="84"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="112"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="149"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="active site" evidence="6">
        <location>
            <position position="139"/>
        </location>
    </feature>
    <feature type="active site" evidence="6">
        <location>
            <position position="157"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU004181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ABF09759.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="178" mass="19904" checksum="11787B9A7AE1DDD0" modified="2006-05-30" version="1">MASSTRSTRSTPARGKSKGASGNVTPWLWMAFALLVVMIDQFFKVVITRSFQYGESRPITSFFNLVLVYNKGAAFSFLADAGGWQRWFFTLLGVVVGAFIIWLLYRHTGQKLFCLAVSLILGGAVGNVVDRIIHGHVIDFLDFHLRNTHFPAFNVADCAITLGAILLIVDELRRVRRH</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LJF6</accession>
    <name>Q1LJF6_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Tyr recombinase activity site-specific recombination Tyr recombinase activity CMGI-5</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">int</name>
        <name evidence="7" type="ordered locus">Rmet_2847</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09720.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2847"/>
    <dbReference type="EnsemblBacteria" id="ABF09720">
        <property type="protein sequence ID" value="ABF09720"/>
        <property type="gene ID" value="Rmet_2847"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2847"/>
    <dbReference type="eggNOG" id="COG0582">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027562_32_1_4"/>
    <dbReference type="OMA" id="LMRYYHP"/>
    <dbReference type="OrthoDB" id="785253at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.443.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044068">
        <property type="entry name" value="CB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011010">
        <property type="entry name" value="DNA_brk_join_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013762">
        <property type="entry name" value="Integrase-like_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002104">
        <property type="entry name" value="Integrase_catalytic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010998">
        <property type="entry name" value="Integrase_recombinase_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00589">
        <property type="entry name" value="Phage_integrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56349">
        <property type="entry name" value="SSF56349"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51900">
        <property type="entry name" value="CB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51898">
        <property type="entry name" value="TYR_RECOMBINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0229">DNA integration</keyword>
    <keyword evidence="3" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="2 4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Core-binding (CB)" evidence="6">
        <location>
            <begin position="73"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="domain" description="Tyr recombinase" evidence="5">
        <location>
            <begin position="183"/>
            <end position="353"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABF09720.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="366" mass="40975" checksum="17B62B5429115A95" modified="2006-05-30" version="1">MGKSRAIESGIYQRGPYSFQVKLMVDGHRVTGTFDTLDEARAYRNSKRAALALDPDARRVLDARVKRHEVKAATLSLALDRYEKEVSAKKKSAESEAHKITRLKRSKLAKMSLYRITADDVTAFLKDLKRDQPGPLQGQPLSDATKRKLVALLSHVFTIARKRWRMAVANPVADIELPSHGKSRKRRLEEGEEQRLLRELAKARQAAIVVPLVRLAIETAMRQGELLALEWKDLRIADTHGTAMLHDTKNGEPRIVPLNATACEILGAMPRPLKGGPVFPIKTAALRAIWEFACERASIEGLRFHDLRHEATSRLFELGLGRIEAASITGHKTLQVLKNYSHLRAEWLAQKMNQAASSQGLAARQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="114" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LK25</accession>
    <name>Q1LK25_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 13">DNA polymerase I</fullName>
            <ecNumber evidence="3 13">2.7.7.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13 17" type="primary">polA</name>
        <name evidence="17" type="ordered locus">Rmet_2624</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">In addition to polymerase activity, this DNA polymerase exhibits 5'-3' exonuclease activity.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12 13">
            <text>a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) = diphosphate + DNA(n+1)</text>
            <dbReference type="Rhea" id="RHEA:22508"/>
            <dbReference type="Rhea" id="RHEA-COMP:17339"/>
            <dbReference type="Rhea" id="RHEA-COMP:17340"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61560"/>
            <dbReference type="ChEBI" id="CHEBI:173112"/>
            <dbReference type="EC" id="2.7.7.7"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="2">Single-chain monomer with multiple functions.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 13">Belongs to the DNA polymerase type-A family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.7" evidence="3 13"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09501.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517202.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2624"/>
    <dbReference type="EnsemblBacteria" id="ABF09501">
        <property type="protein sequence ID" value="ABF09501"/>
        <property type="gene ID" value="Rmet_2624"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2624"/>
    <dbReference type="eggNOG" id="COG0258">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG0749">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004675_0_0_4"/>
    <dbReference type="OMA" id="NRPPMPD"/>
    <dbReference type="OrthoDB" id="1220182at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008408">
        <property type="term" value="F:3'-5' exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003887">
        <property type="term" value="F:DNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006261">
        <property type="term" value="P:DNA-templated DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd09898">
        <property type="entry name" value="H3TH_53EXO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002562">
        <property type="entry name" value="3'-5'_exonuclease_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020046">
        <property type="entry name" value="5-3_exonucl_a-hlix_arch_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002421">
        <property type="entry name" value="5-3_exonuclease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036279">
        <property type="entry name" value="5-3_exonuclease_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019760">
        <property type="entry name" value="DNA-dir_DNA_pol_A_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001098">
        <property type="entry name" value="DNA-dir_DNA_pol_A_palm_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020045">
        <property type="entry name" value="DNA_polI_H3TH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018320">
        <property type="entry name" value="DNA_polymerase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002298">
        <property type="entry name" value="DNA_polymerase_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008918">
        <property type="entry name" value="HhH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029060">
        <property type="entry name" value="PIN-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10133">
        <property type="entry name" value="PTHR10133"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01367">
        <property type="entry name" value="5_3_exonuc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02739">
        <property type="entry name" value="5_3_exonuc_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00476">
        <property type="entry name" value="DNA_pol_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01612">
        <property type="entry name" value="DNA_pol_A_exo1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00868">
        <property type="entry name" value="DNAPOLI"/>
    </dbReference>
    <dbReference type="SMART" id="SM00474">
        <property type="entry name" value="35EXOc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00475">
        <property type="entry name" value="53EXOc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00279">
        <property type="entry name" value="HhH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00482">
        <property type="entry name" value="POLAc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47807">
        <property type="entry name" value="SSF47807"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="SSF53098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="SSF56672"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88723">
        <property type="entry name" value="SSF88723"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00593">
        <property type="entry name" value="pola"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00447">
        <property type="entry name" value="DNA_POLYMERASE_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-0227">DNA damage</keyword>
    <keyword evidence="13" id="KW-0234">DNA repair</keyword>
    <keyword evidence="13" id="KW-0235">DNA replication</keyword>
    <keyword evidence="11 13" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="10 13" id="KW-0239">DNA-directed DNA polymerase</keyword>
    <keyword evidence="9 17" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0540">Nuclease</keyword>
    <keyword evidence="6 13 17" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 13 17" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="53EXOc" evidence="15">
        <location>
            <begin position="4"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="domain" description="3'-5' exonuclease" evidence="14">
        <location>
            <begin position="332"/>
            <end position="523"/>
        </location>
    </feature>
    <feature type="domain" description="POLAc" evidence="16">
        <location>
            <begin position="692"/>
            <end position="898"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011541"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00020311"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00024632"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU004460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM00474"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="SMART" id="SM00475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="SMART" id="SM00482"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="ABF09501.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="934" mass="102529" checksum="A811701C5053DE56" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="68" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LK82</accession>
    <name>Q1LK82_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Membrane protein TerC family, possibly involved in tellurium resistance</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_2567</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09444.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517147.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2567"/>
    <dbReference type="EnsemblBacteria" id="ABF09444">
        <property type="protein sequence ID" value="ABF09444"/>
        <property type="gene ID" value="Rmet_2567"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2567"/>
    <dbReference type="eggNOG" id="COG0861">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_070543_0_1_4"/>
    <dbReference type="OMA" id="YTAGKMI"/>
    <dbReference type="OrthoDB" id="1917125at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005496">
        <property type="entry name" value="Integral_membrane_TerC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022301">
        <property type="entry name" value="Integral_membrane_YjbE"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03741">
        <property type="entry name" value="TerC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03717">
        <property type="entry name" value="R_switched_YjbE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="49"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="77"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="106"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="131"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="167"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="201"/>
            <end position="220"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF09444.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="232" mass="24507" checksum="F374287C05B0A075" modified="2006-05-30" version="1">MEWFTDLFTMQFLTALLSIVVIDLVLAGDNAIVIALAARNLPPHLQKKAIVWGTIGAVVVRSAMTIGVVWLLKIPGLLLVGGLALVWIAYKLLAAEEDGDEHGSGASTLVGAMKTIIIADAVMGVDNVLAVAGAAHGSFLLVVLGLLISIPIVVWGSSLVLKLMARFPSIIYVGAGVLAFTAVKMILGEPITKPFFEAQPIIKWALYLVIVGGVLGAGYLTQKRREPSTQAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="95" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LKB7</accession>
    <name>Q1LKB7_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Acetyl-CoA C-acyltransferase</fullName>
            <ecNumber evidence="6">2.3.1.16</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Rmet_2532</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the thiolase-like superfamily. Thiolase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.16" evidence="6"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09409.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517108.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2532"/>
    <dbReference type="EnsemblBacteria" id="ABF09409">
        <property type="protein sequence ID" value="ABF09409"/>
        <property type="gene ID" value="Rmet_2532"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2532"/>
    <dbReference type="eggNOG" id="COG0183">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035425_0_0_4"/>
    <dbReference type="OMA" id="QHAFYDV"/>
    <dbReference type="OrthoDB" id="311458at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003988">
        <property type="term" value="F:acetyl-CoA C-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.47.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002155">
        <property type="entry name" value="Thiolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016039">
        <property type="entry name" value="Thiolase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020615">
        <property type="entry name" value="Thiolase_acyl_enz_int_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020617">
        <property type="entry name" value="Thiolase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020613">
        <property type="entry name" value="Thiolase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020616">
        <property type="entry name" value="Thiolase_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02803">
        <property type="entry name" value="Thiolase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00108">
        <property type="entry name" value="Thiolase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000429">
        <property type="entry name" value="Ac-CoA_Ac_transf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53901">
        <property type="entry name" value="SSF53901"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00098">
        <property type="entry name" value="THIOLASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00737">
        <property type="entry name" value="THIOLASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3 6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Thiolase_N" evidence="4">
        <location>
            <begin position="20"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="domain" description="Thiolase_C" evidence="5">
        <location>
            <begin position="272"/>
            <end position="364"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF02803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABF09409.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="395" mass="41892" checksum="4EC9FD7AF5A42FD3" modified="2006-05-30" version="1">MSEKVLVGGVGMIPFSKPGASPSYTDMGAEAVRRALADAGIGYEQVQQAYVGYVYGDSTCGQTALYEVGLTGIPVVNVNNNCSTGSTALYLARQAVALGDADCVLALGFEQMQAGALKSHWDDRPKTRARFAHVLQDLTADVADLPQALRTFGGAGREHMQKYGTKMETFAAIRAKASRHAANNPLALFRNVVTTEEVMNDKVMWPGVMTRLMACPPTCGAAAALIVSEKFAKKHGLRTDVQILAQAMATDPVESFDPPSMISYAGFHMAQAAANKVYEKAGVNPKDIRVAELHDCFAHNELLSYEALGLCGIGEGEKFVMDGDNTYGGRVVTNSSGGLLSKGHPLGATGLAQCYELTHQLRGTADKRQVEGAKLALQHNIGLGGACVVTLYGKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LKK1</accession>
    <name>Q1LKK1_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">LUD_dom domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_2448</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09325.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517041.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2448"/>
    <dbReference type="EnsemblBacteria" id="ABF09325">
        <property type="protein sequence ID" value="ABF09325"/>
        <property type="gene ID" value="Rmet_2448"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2448"/>
    <dbReference type="eggNOG" id="COG1556">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_090664_3_0_4"/>
    <dbReference type="OMA" id="YRVHVIL"/>
    <dbReference type="OrthoDB" id="1580190at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10420">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024185">
        <property type="entry name" value="FTHF_cligase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003741">
        <property type="entry name" value="LUD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037171">
        <property type="entry name" value="NagB/RpiA_transferase-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02589">
        <property type="entry name" value="LUD_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100950">
        <property type="entry name" value="SSF100950"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="LUD_dom" evidence="1">
        <location>
            <begin position="129"/>
            <end position="225"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02589"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF09325.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="228" mass="24279" checksum="F35A1E21AD26B45A" modified="2006-05-30" version="1">METNAARDRIFARIRAAQGRGPAVTEGEREAVADYLARHPQGPQPPVDGDRTAGFVAQAQKMASTVDRVATLSEVPAAVARYVDGLKLVRRAVAWTSLGSLPWAEQGMTVECRPPLRDPQGDHDHGDLVGITGCFVAIAETGSLMLLSGPDTFASAALLPETHIAVVPVSRIVANLEEAFAQMRAEHGRLPRATNIISGPSRTGDIEQTIVLGAHGPYRVHVILVDQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LKL6</accession>
    <name>Q1LKL6_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Large ribosomal RNA subunit accumulation protein YceD</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">23S rRNA accumulation protein YceD</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Rmet_2433</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Plays a role in synthesis, processing and/or stability of 23S rRNA.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the DUF177 domain family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09310.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517026.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2433"/>
    <dbReference type="EnsemblBacteria" id="ABF09310">
        <property type="protein sequence ID" value="ABF09310"/>
        <property type="gene ID" value="Rmet_2433"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2433"/>
    <dbReference type="eggNOG" id="COG1399">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_094127_0_0_4"/>
    <dbReference type="OMA" id="CMTPYDQ"/>
    <dbReference type="OrthoDB" id="1665714at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003772">
        <property type="entry name" value="YceD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039255">
        <property type="entry name" value="YceD_bac"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38099">
        <property type="entry name" value="PTHR38099"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02620">
        <property type="entry name" value="YceD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="174"/>
            <end position="205"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002868"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010740"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00015716"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00031841"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABF09310.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="205" mass="21804" checksum="FE435540B1C41C08" modified="2006-05-30" version="1">MSQPTQPVASAGAFDLRETDLFAFCRKGSNATGDVATRDLPRILAETAADAPASAPDETFHYTVAGSVRDEAVEPGTPVVQRLFLDVAVNGRVWLDCQRCLGVYAEPIATEMCFEVVVSEAAAEAAPMDDDETDVIVGSKKFSVLELIEDEVLLALPTAPKHAVCPSVHESLVTGADGEVEPEAPPEEEKRPSPFAALASLKTKH</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-01-15" modified="2022-05-25" version="99" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LKN7</accession>
    <name>EFP_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Elongation factor P</fullName>
            <shortName evidence="1">EF-P</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">efp</name>
        <name type="ordered locus">Rmet_2412</name>
    </gene>
    <organism>
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in peptide bond synthesis. Stimulates efficient translation and peptide-bond synthesis on native or reconstituted 70S ribosomes in vitro. Probably functions indirectly by altering the affinity of the ribosome for aminoacyl-tRNA, thus increasing their reactivity as acceptors for peptidyl transferase.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Protein biosynthesis; polypeptide chain elongation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the elongation factor P family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09289.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008642375.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q1LKN7"/>
    <dbReference type="SMR" id="Q1LKN7"/>
    <dbReference type="STRING" id="266264.Rmet_2412"/>
    <dbReference type="EnsemblBacteria" id="ABF09289">
        <property type="protein sequence ID" value="ABF09289"/>
        <property type="gene ID" value="Rmet_2412"/>
    </dbReference>
    <dbReference type="GeneID" id="60821114"/>
    <dbReference type="KEGG" id="rme:Rmet_2412"/>
    <dbReference type="eggNOG" id="COG0231">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_074944_2_1_4"/>
    <dbReference type="OMA" id="LYRMRMY"/>
    <dbReference type="OrthoDB" id="1260763at2"/>
    <dbReference type="UniPathway" id="UPA00345"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd04470">
        <property type="entry name" value="S1_EF-P_repeat_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05794">
        <property type="entry name" value="S1_EF-P_repeat_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00141">
        <property type="entry name" value="EF_P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015365">
        <property type="entry name" value="Elong-fact-P_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014722">
        <property type="entry name" value="Rib_L2_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020599">
        <property type="entry name" value="Transl_elong_fac_P/YeiP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013185">
        <property type="entry name" value="Transl_elong_KOW-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001059">
        <property type="entry name" value="Transl_elong_P/YeiP_cen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013852">
        <property type="entry name" value="Transl_elong_P/YeiP_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011768">
        <property type="entry name" value="Transl_elongation_fac_P"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008991">
        <property type="entry name" value="Translation_prot_SH3-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30053">
        <property type="entry name" value="PTHR30053"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01132">
        <property type="entry name" value="EFP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08207">
        <property type="entry name" value="EFP_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09285">
        <property type="entry name" value="Elong-fact-P_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005901">
        <property type="entry name" value="EF-P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01185">
        <property type="entry name" value="EFP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00841">
        <property type="entry name" value="Elong-fact-P_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50104">
        <property type="entry name" value="SSF50104"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00038">
        <property type="entry name" value="efp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01275">
        <property type="entry name" value="EFP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0251">Elongation factor</keyword>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_1000010825" description="Elongation factor P">
        <location>
            <begin position="1"/>
            <end position="186"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00141"/>
        </source>
    </evidence>
    <sequence length="186" mass="21071" checksum="48D08896B9ADC13E" modified="2006-05-30" version="1">MKIAQELRVGNVFMIGSDPMVVQKAEYNKSGRNAAVVKMKYKNLLTEAPGESVFKADDKFEVVVLERRECTYSYFADPMYVFMDTEYNQYEVEKDSMGDSLNYLEDGMVVEVVFYNDKAISVEMPTTLVREIIYTEPAVKGDTSSGKVLKGAKINTGFELQVPLFCNIGDKIEIDTRTGEYRSRAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="65" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LKW8</accession>
    <name>Q1LKW8_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">PilL lipoprotein (Type IV pili)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">pilL</name>
        <name evidence="2" type="ordered locus">Rmet_2331</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09208.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516994.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2331"/>
    <dbReference type="EnsemblBacteria" id="ABF09208">
        <property type="protein sequence ID" value="ABF09208"/>
        <property type="gene ID" value="Rmet_2331"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2331"/>
    <dbReference type="eggNOG" id="COG3266">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_068440_3_0_4"/>
    <dbReference type="OMA" id="WFHYPER"/>
    <dbReference type="OrthoDB" id="1941902at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022260">
        <property type="entry name" value="Integr_conj_element_PilL"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03748">
        <property type="entry name" value="conj_PilL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="170"/>
            <end position="199"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF09208.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="199" mass="21405" checksum="94818C08A13FEFA7" modified="2006-05-30" version="1">MCPSPPWFHYPERRLLAGFLGLLWSVLAGGCATTTAPVAPDTIAEVLAAPEPEASEYIPVVRYARYTLVELAPLAAQRDLLLQTIDVSMPEDARATVGDGLRHVLKRSGYGLCQTAHAVIELYALPLPAVHLHLGPMTLRDALLTLAGPAWELHADDRARQICFERPGDRAVAESPSEPPATEAVQTFPLAPMVSGGQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="54" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LKY0</accession>
    <name>Q1LKY0_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_2319</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09196.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_003098923.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2319"/>
    <dbReference type="EnsemblBacteria" id="ABF09196">
        <property type="protein sequence ID" value="ABF09196"/>
        <property type="gene ID" value="Rmet_2319"/>
    </dbReference>
    <dbReference type="GeneID" id="56409826"/>
    <dbReference type="GeneID" id="61526443"/>
    <dbReference type="KEGG" id="rme:Rmet_2319"/>
    <dbReference type="eggNOG" id="ENOG503067M">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2379609_0_0_4"/>
    <dbReference type="OrthoDB" id="1751798at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="73"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="80"/>
            <end position="94"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF09196.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="94" mass="10034" checksum="E33A09433442C6D1" modified="2006-05-30" version="1">MSAARSRGTWTLEVTRLCTDGTPSACSKLYGAAWQAARALGYIRLLTYTMPDEGGASLRAAGWRLIGARGGGAWSRPGRPRADTPEHLRGAKCL</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="59" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LL05</accession>
    <name>Q1LL05_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_2292</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09171.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004350508.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2292"/>
    <dbReference type="EnsemblBacteria" id="ABF09171">
        <property type="protein sequence ID" value="ABF09171"/>
        <property type="gene ID" value="Rmet_2292"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2292"/>
    <dbReference type="eggNOG" id="COG1475">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028960_2_0_4"/>
    <dbReference type="OMA" id="QPHISRM"/>
    <dbReference type="OrthoDB" id="174505at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022304">
        <property type="entry name" value="ICE_PFGI_1_ParB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036086">
        <property type="entry name" value="ParB/Sulfiredoxin_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110849">
        <property type="entry name" value="SSF110849"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03764">
        <property type="entry name" value="ICE_PFGI_1_parB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="295"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="305"/>
            <end position="319"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF09171.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="559" mass="60182" checksum="5B8A46A238CF31F5" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="95" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LL28</accession>
    <name>Q1LL28_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Na+/solute symporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">Rmet_2269</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the sodium:solute symporter (SSF) (TC 2.A.21) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09148.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516973.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2269"/>
    <dbReference type="EnsemblBacteria" id="ABF09148">
        <property type="protein sequence ID" value="ABF09148"/>
        <property type="gene ID" value="Rmet_2269"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2269"/>
    <dbReference type="eggNOG" id="COG4147">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018808_8_2_4"/>
    <dbReference type="OMA" id="GTTWVQM"/>
    <dbReference type="OrthoDB" id="278483at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1730.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038377">
        <property type="entry name" value="Na/Glc_symporter_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001734">
        <property type="entry name" value="Na/solute_symporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019899">
        <property type="entry name" value="Na/solute_symporter_VC_2705"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00474">
        <property type="entry name" value="SSF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03648">
        <property type="entry name" value="Na_symport_lg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50283">
        <property type="entry name" value="NA_SOLUT_SYMP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
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        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="42"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="83"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="111"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="157"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="189"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="222"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="379"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="413"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="500"/>
            <end position="529"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="550"/>
            <end position="567"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="573"/>
            <end position="597"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="604"/>
            <end position="625"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="645"/>
            <end position="665"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="351"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="354"/>
            <end position="370"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00006434"/>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="RuleBase" id="RU362091"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ABF09148.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="683" mass="74797" checksum="7CAA29BE3D44C139" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LL38</accession>
    <name>Q1LL38_CUPMC</name>
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        <recommendedName>
            <fullName evidence="2">CBS domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_2259</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
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        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09138.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2259"/>
    <dbReference type="EnsemblBacteria" id="ABF09138">
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        <property type="gene ID" value="Rmet_2259"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2259"/>
    <dbReference type="eggNOG" id="COG2208">
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    <dbReference type="HOGENOM" id="CLU_694159_0_0_4"/>
    <dbReference type="OMA" id="AFMDKEP"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="CBS_dom_sf"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0129">CBS domain</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="23"/>
            <end position="80"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF09138.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="415" mass="45610" checksum="C6F0AF3C7E5AC1BE" modified="2006-05-30" version="1">MSGVLVDPSAARPRFRQPCAEDLAMPTPSVTTDQTNQDVLEVFAAHRDLACLPVTEQGKPIGLINRSIFLSQMSKPYHRELYNRKSCIAFMDKEPLIVDAAMSIDALTFRTVEYGEKALADGFIIARDDQYIGLGQGLQLMRVVADMQAERNRQIMHSIDYASTIQRAMLRASREMLGATLPDAALVWEPRDVVGGDFYHFAAFEDGWFAALADCTGHGVPGAFMTLIASSLLTQAIEQIGPRDPGALLMAVNRGIKQMLGQNDREPRESPSASESDDGMDAAFFWFDNATRQLTFAGAKSSLFVLQPADAEGEMINGARMGVGYVDTDMAFTWQNSVAPTTSGTLVFVTTDGLIDQIGGPKEIAFGKRRMREAICANRAASATQISQAVQDAHRDWQGTQRRRDDLTFFCFRAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LL51</accession>
    <name>Q1LL51_CUPMC</name>
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        <submittedName>
            <fullName evidence="5">Putative outer membrane lipoprotein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Rmet_2246</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
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        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="ABF09125.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2246"/>
    <dbReference type="EnsemblBacteria" id="ABF09125">
        <property type="protein sequence ID" value="ABF09125"/>
        <property type="gene ID" value="Rmet_2246"/>
    </dbReference>
    <dbReference type="GeneID" id="60821181"/>
    <dbReference type="KEGG" id="rme:Rmet_2246"/>
    <dbReference type="eggNOG" id="COG2885">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016890_12_3_4"/>
    <dbReference type="OMA" id="TDEGWAF"/>
    <dbReference type="OrthoDB" id="2018797at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07185">
        <property type="entry name" value="OmpA_C-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006664">
        <property type="entry name" value="OMP_bac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006665">
        <property type="entry name" value="OmpA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036737">
        <property type="entry name" value="OmpA-like_sf"/>
    </dbReference>
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        <property type="entry name" value="TAT_signal"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00691">
        <property type="entry name" value="OmpA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01021">
        <property type="entry name" value="OMPADOMAIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103088">
        <property type="entry name" value="SSF103088"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="TAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="1 2" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193645" evidence="3">
        <location>
            <begin position="34"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="domain" description="OmpA-like" evidence="4">
        <location>
            <begin position="52"/>
            <end position="167"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABF09125.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="167" mass="18009" checksum="726CCB61F77D47EA" modified="2006-05-30" version="1">MRAPSRPRRAFLLTTLAGLGALALSGCASPAHKLTPAQIAVLKNQGFMLTGNGWELVMPDKVLFGFDDDTVSAEQQNALARVARTLRDAGIDALRVDGHTDNVGTIEYNQQLSMRRAEAVARVLLTCGFARDHLDVRGFGKTRPIADNGTALGRAENRRVAIIVTVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="108" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LL64</accession>
    <name>Q1LL64_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="10">ABC-type transporter, ATPase component</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">Rmet_2233</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09112.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516939.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2233"/>
    <dbReference type="EnsemblBacteria" id="ABF09112">
        <property type="protein sequence ID" value="ABF09112"/>
        <property type="gene ID" value="Rmet_2233"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2233"/>
    <dbReference type="eggNOG" id="COG3839">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_1_4"/>
    <dbReference type="OMA" id="YDTMTVF"/>
    <dbReference type="OrthoDB" id="1200451at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015408">
        <property type="term" value="F:ABC-type ferric iron transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd03259">
        <property type="entry name" value="ABC_Carb_Solutes_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015853">
        <property type="entry name" value="ABC_transpr_FbpC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008995">
        <property type="entry name" value="Mo/tungstate-bd_C_term_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040582">
        <property type="entry name" value="OB_MalK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17912">
        <property type="entry name" value="OB_MalK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50331">
        <property type="entry name" value="SSF50331"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-1278">Translocase</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ABC transporter" evidence="9">
        <location>
            <begin position="6"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="41"/>
            <end position="48"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ABF09112.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="366" mass="40087" checksum="949A186B2EADC397" modified="2006-05-30" version="1">MARIDLDLAHAYVANPQKDEDYALLPLHFTFEDGGAYALLGPSGCGKTTLLNCISGLLRPSQGTISFDGKDVTGDSPQARNIAQVFQFPVIYDTMTVGENLAFPLRNRGVPAAQVKERVGRVAEMLDLSASLDRRASGLAADAKQKISLGRGLVRQDVSAILFDEPLTVIDPHLKWQLRRKLKEIHHEFRLTLIYVTHDQTEALTFADQVVVMSRGKAVQVGSADALFERPAHTFVGHFIGSPGMNFLPGQWRDGGIEMAGRRYAPDLSPDVAARLQAAGSFKVGVRPEYLKLAAGNDPQALPVRVERAQDIGTYWLVSSTVDHGEAATLRARLGTEAAHLRAGDTAWLSVFNRHTCFYVNEELVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="97" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LLB0</accession>
    <name>Q1LLB0_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Dihydropteroate synthase</fullName>
            <shortName evidence="1">DHPS</shortName>
            <ecNumber evidence="1">2.5.1.15</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Dihydropteroate pyrophosphorylase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="3" type="primary">folP</name>
        <name evidence="3" type="ordered locus">Rmet_2187</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the condensation of para-aminobenzoate (pABA) with 6-hydroxymethyl-7,8-dihydropterin diphosphate (DHPt-PP) to form 7,8-dihydropteroate (H2Pte), the immediate precursor of folate derivatives.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; tetrahydrofolate biosynthesis; 7,8-dihydrofolate from 2-amino-4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine diphosphate and 4-aminobenzoate: step 1/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the DHPS family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.15" evidence="1"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09066.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516894.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2187"/>
    <dbReference type="EnsemblBacteria" id="ABF09066">
        <property type="protein sequence ID" value="ABF09066"/>
        <property type="gene ID" value="Rmet_2187"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2187"/>
    <dbReference type="eggNOG" id="COG0294">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008023_0_3_4"/>
    <dbReference type="OMA" id="FSIDTYH"/>
    <dbReference type="OrthoDB" id="1275854at2"/>
    <dbReference type="UniPathway" id="UPA00077">
        <property type="reaction ID" value="UER00156"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004156">
        <property type="term" value="F:dihydropteroate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046656">
        <property type="term" value="P:folic acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046654">
        <property type="term" value="P:tetrahydrofolate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00739">
        <property type="entry name" value="DHPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045031">
        <property type="entry name" value="DHP_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006390">
        <property type="entry name" value="DHP_synth_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011005">
        <property type="entry name" value="Dihydropteroate_synth-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000489">
        <property type="entry name" value="Pterin-binding_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20941">
        <property type="entry name" value="PTHR20941"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00809">
        <property type="entry name" value="Pterin_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51717">
        <property type="entry name" value="SSF51717"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01496">
        <property type="entry name" value="DHPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00792">
        <property type="entry name" value="DHPS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00793">
        <property type="entry name" value="DHPS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50972">
        <property type="entry name" value="PTERIN_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0289">Folate biosynthesis</keyword>
    <keyword evidence="1" id="KW-0460">Magnesium</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Pterin-binding" evidence="2">
        <location>
            <begin position="19"/>
            <end position="271"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU361205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50972"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF09066.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="289" mass="31005" checksum="BB76F0BA4C53D62E" modified="2006-05-30" version="1">MSTEFFQCGRYRFSRRRRPLVMGILNVTPDSFSDGGQHATRDAALRHAEQLIAEGVDIIDIGGESSRPGSAALPLDQELDRVMPIVESLADCGKPLSIDTYKPEVMRAALAGGADLINDIWGFRMPGAVEAVAAGQFGLCVMHMQRDPQTMQEDPHYTDVVAEVASFLEERVSTLRAAGIDTARISLDPGFGFGKTPDHNLRLLGQLPSLAVDGLPILVGLSRKSTLGAIIGGKPPQARVAASVAAAVCAAERGAYIVRVHDVEQTVDALKVWWAVRQESVAPDGQHGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LLB2</accession>
    <name>Q1LLB2_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 6">Phosphate-binding protein PstS</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">pstS</name>
        <name evidence="9" type="ordered locus">Rmet_2185</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 6">Part of the ABC transporter complex PstSACB involved in phosphate import.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3 6">The complex is composed of two ATP-binding proteins (PstB), two transmembrane proteins (PstC and PstA) and a solute-binding protein (PstS).</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the PstS family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09064.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516892.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2185"/>
    <dbReference type="EnsemblBacteria" id="ABF09064">
        <property type="protein sequence ID" value="ABF09064"/>
        <property type="gene ID" value="Rmet_2185"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2185"/>
    <dbReference type="eggNOG" id="COG0226">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_034528_1_0_4"/>
    <dbReference type="OMA" id="FPYPVYA"/>
    <dbReference type="OrthoDB" id="1222928at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043190">
        <property type="term" value="C:ATP-binding cassette (ABC) transporter complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042301">
        <property type="term" value="F:phosphate ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035435">
        <property type="term" value="P:phosphate ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005673">
        <property type="entry name" value="ABC_phos-bd_PstS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024370">
        <property type="entry name" value="PBP_domain"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12849">
        <property type="entry name" value="PBP_like_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002756">
        <property type="entry name" value="PstS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00975">
        <property type="entry name" value="3a0107s03"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0592">Phosphate transport</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193649" description="Phosphate-binding protein PstS" evidence="7">
        <location>
            <begin position="23"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="domain" description="PBP_domain" evidence="8">
        <location>
            <begin position="22"/>
            <end position="304"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002841"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00021889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR002756"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF12849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ABF09064.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="343" mass="36292" checksum="83EA266E536AF97E" modified="2006-05-30" version="1">MKLVKTAVAGIVSIVVAGTAFAAEITGAGASFPAPVYSKWADAYQKATGNKVNYQSIGSSGGIKQIGAKTVDFGASDAPLKDDELAKGNLIQFPTVIGGVVPVINLQGVKPGELIITGDVLANIYLGKIKKWDDPAIKALNPKVHLPSQDILPVRRADGSGTTFIFTNYLSKVSPDWKNSVGEGTTVNWPGGGTGGKGNEGVAAFVQRLNGAIGYVEYAYAKQNKMTYLTMKNSAGEIVKPSDEAFKAAAAGADWSKSFYQILTNEPGKTAWPIAGATFILLHKTQDKAAQGQEVMKFFDWAYKSGGTMAAELDYVPLPESVVNQIRSTWKSSVKDGSGKALY</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="101" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LMF5</accession>
    <name>Q1LMF5_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="9">ABC transporter related, ATP binding protein</fullName>
            <ecNumber evidence="9">3.6.3.30</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">ybbA</name>
        <name evidence="9" type="ordered locus">Rmet_1792</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EC" id="3.6.3.30" evidence="9"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08671.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516521.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1792"/>
    <dbReference type="EnsemblBacteria" id="ABF08671">
        <property type="protein sequence ID" value="ABF08671"/>
        <property type="gene ID" value="Rmet_1792"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1792"/>
    <dbReference type="eggNOG" id="COG1136">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_22_4"/>
    <dbReference type="OMA" id="FNWPGHP"/>
    <dbReference type="OrthoDB" id="1181903at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03255">
        <property type="entry name" value="ABC_MJ0796_LolCDE_FtsE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017911">
        <property type="entry name" value="MacB_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="1" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-1278">Translocase</keyword>
    <feature type="domain" description="ABC transporter" evidence="8">
        <location>
            <begin position="7"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="43"/>
            <end position="50"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ABF08671.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="234" mass="25280" checksum="1A5FAE2AB41D8B02" modified="2006-05-30" version="1">MSAAPLVSIRHLAKSYQRGAQTVPVLSDISLDIAEGDFIALMGPSGSGKSTLLNLIAGIDRPDSGELIVGGVDISDLPESALADWRAAHVGFIFQFYNLMPVLTAFENVELPLMLTRLSRAERRERVELVLRMVNLADRMDHYPSELSGGQQQRVAIARALITDPTLIVADEPTGDLDRTSATEILTMLQRLNADVGKTIIMVTHDAHAAEAAKSLVHLEKGELTNSVATTASR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="103" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LMI1</accession>
    <name>Q1LMI1_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 7">Glutamate decarboxylase</fullName>
            <ecNumber evidence="3 7">4.1.1.15</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">gadB</name>
        <name evidence="8" type="ordered locus">Rmet_1766</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 7">
            <text>H(+) + L-glutamate = 4-aminobutanoate + CO2</text>
            <dbReference type="Rhea" id="RHEA:17785"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:59888"/>
            <dbReference type="EC" id="4.1.1.15"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 5 6">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the group II decarboxylase family.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.15" evidence="3 7"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08645.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516498.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1766"/>
    <dbReference type="EnsemblBacteria" id="ABF08645">
        <property type="protein sequence ID" value="ABF08645"/>
        <property type="gene ID" value="Rmet_1766"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1766"/>
    <dbReference type="eggNOG" id="COG0076">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019582_2_2_4"/>
    <dbReference type="OMA" id="TMAPHSE"/>
    <dbReference type="OrthoDB" id="1478871at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004351">
        <property type="term" value="F:glutamate decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006536">
        <property type="term" value="P:glutamate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010107">
        <property type="entry name" value="Glutamate_decarboxylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002129">
        <property type="entry name" value="PyrdxlP-dep_de-COase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43321">
        <property type="entry name" value="PTHR43321"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00282">
        <property type="entry name" value="Pyridoxal_deC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="SSF53383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01788">
        <property type="entry name" value="Glu-decarb-GAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0210">Decarboxylase</keyword>
    <keyword evidence="4 6 8" id="KW-0456">Lyase</keyword>
    <keyword evidence="5 6" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="5">
        <location>
            <position position="267"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012421"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR602129-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU361171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ABF08645.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="460" mass="52338" checksum="65F6E8AD4E912697" modified="2006-05-30" version="1">MSDMQEHKVTPYDQFNYEVPENVFPREGISANAAAAIVISDEWTDTNPMLNMSSFVTTFAEPEAREVAARNIFKNYIDHDMYPRLFAMEGRMVRWLHELWNGPKGVEPYGTCTVGSSEACMLGGLAHKWNWRQRRQAEGKDATRPNMVTGGNVQIVWKKFLRYFDVEPRIVPLKPGNYCLTAEDLDKYVDENTICVVAIAGQTFTGEDDDIQGIHDWLDAYEKRTGISIPMHIDGASGGFVNPFLYPDYKWDFRLPRVQSINASGHKYGLTPPGLGWVVFRERKIFNEELVFYVNYLGGEMPTATLNFSRNAFQVAVQYYQFLRLGFDGYKRVMQHTLDNAIFLRQQLVDSGYFDIMNTTQRIPVVAVTLNKKYKNFNEFDVSNKVREKGWVLSAYTMPPNAESVTSLRVVVRPHVNHNVAQLLANDIINACKFLEANGGNAKPPALHKHAEAQSTSLKC</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="66" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LMI9</accession>
    <name>Q1LMI9_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_1758</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08637.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1758"/>
    <dbReference type="EnsemblBacteria" id="ABF08637">
        <property type="protein sequence ID" value="ABF08637"/>
        <property type="gene ID" value="Rmet_1758"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1758"/>
    <dbReference type="eggNOG" id="COG4313">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_077940_0_0_4"/>
    <dbReference type="OMA" id="LQGHLIY"/>
    <dbReference type="OrthoDB" id="1322015at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025737">
        <property type="entry name" value="Phenol_MetA_deg_put"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13557">
        <property type="entry name" value="Phenol_MetA_deg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193218" evidence="1">
        <location>
            <begin position="27"/>
            <end position="306"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF08637.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="306" mass="32866" checksum="D1123544AF9EC7DC" modified="2006-05-30" version="1">MVCPGRTLVTLWMWLALWCCTMPAHAQELEPRSYSAAPVGTNFLAATYTRLSGDVLTDPSLPITDVRSRINIFGAGYVHVFDLAGRTASIGLLAPFASGDVSGNVLDAPTAVHRAGIGDARVRLAINLLGGPALTPAEFMKRAPDTMLGASLTVVAPTGQYDASRLVNVGTHRWAFKPEIGLSQPIGNWFSELSAGVWLFGDNNAFLGDKRREQSPLGVLQWHVGYQFRPALWIAADAGFYSGGRTSVNGIPNADRQQNSRYGVTLSVPIVPGWSAKLAASKGLITRAAGDYTAITLALQYQWINH</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LML4</accession>
    <name>Q1LML4_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Outer membrane protein (Porin)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Rmet_1733</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08612.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516465.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1733"/>
    <dbReference type="EnsemblBacteria" id="ABF08612">
        <property type="protein sequence ID" value="ABF08612"/>
        <property type="gene ID" value="Rmet_1733"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1733"/>
    <dbReference type="eggNOG" id="COG3203">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038238_2_0_4"/>
    <dbReference type="OMA" id="ANDAWRI"/>
    <dbReference type="OrthoDB" id="1064531at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046930">
        <property type="term" value="C:pore complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015288">
        <property type="term" value="F:porin activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006811">
        <property type="term" value="P:ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00342">
        <property type="entry name" value="gram_neg_porins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.160.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033900">
        <property type="entry name" value="Gram_neg_porin_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023614">
        <property type="entry name" value="Porin_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002299">
        <property type="entry name" value="Porin_Neis"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13609">
        <property type="entry name" value="Porin_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00184">
        <property type="entry name" value="NEISSPPORIN"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0406">Ion transport</keyword>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0626">Porin</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1134">Transmembrane beta strand</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193678" evidence="5">
        <location>
            <begin position="21"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="domain" description="Porin_4" evidence="6">
        <location>
            <begin position="7"/>
            <end position="356"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF13609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABF08612.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="398" mass="41882" checksum="371441820C4163BE" modified="2006-05-30" version="1">MKLKGMAMAALLACTGSAYAQSAVTLYGVLDTSLEFANHVAPYSAAGFTANPGAGNNVFRVNSGGLSGSRWGLRGTEDLGGGLKSIFVLESGISVDNGTMQQSNRLFGRQAFVGISHNTYGQITLGRQYTSLFVAMANFMPTAYATQYEPAAFMAGNNFREDNTIAYTGVFGPVTALAHFSFGAGVPNLAIQGGASAFGGNGEVPGAFRRDSAFGGAFTYSAGPFGAAIAYDQWNPSVNNAAGVYQGTTATLKKASVAGSYAIGQVAKIMAGYRWGKVDNNGTGAQLQRDDYYWIGANYQVTPAVGLTLEYDYDNLKRDYFNNTHAPNPWQIAFVADYSFSKRTDVYLSAAYAKNAGLTLDAANTNQLATTGATGNSYILGTGQSSMVGVSLGLRHKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LN44</accession>
    <name>Q1LN44_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">DNA-binding helix-turn-helix domain-containing protein (CopG-like) (Belongs to the transfer module of genomic island CMGI-3)</fullName>
        </submittedName>
        <submittedName>
            <fullName evidence="1">DNA-binding helix-turn-helix domain-containing protein (CopG-like) (CMGI-2)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_1340</name>
        <name evidence="2" type="ordered locus">Rmet_1549</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="submission" date="2006-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of the chromosome of Ralstonia metallidurans CH34.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Copeland A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Barry K."/>
                <person name="Detter J.C."/>
                <person name="Glavina del Rio T."/>
                <person name="Hammon N."/>
                <person name="Israni S."/>
                <person name="Dalin E."/>
                <person name="Tice H."/>
                <person name="Martinez M."/>
                <person name="Goltsman E."/>
                <person name="Pitluck S."/>
                <person name="Schmutz J."/>
                <person name="Larimer F."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Kim E."/>
                <person name="Mergeay M."/>
                <person name="Benotmane M.A."/>
                <person name="Vallaeys T."/>
                <person name="Michaux A."/>
                <person name="Monchy S."/>
                <person name="Dunn J."/>
                <person name="McCorkle S."/>
                <person name="Taghavi S."/>
                <person name="van der Lelie D."/>
                <person name="Richardson P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CH34</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2010-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Benotmane R."/>
                <person name="Lapidus A."/>
                <person name="McCorkle S."/>
                <person name="Monchy S."/>
                <person name="Taghavi S."/>
                <person name="van der Lelie N."/>
                <person name="Dunn J."/>
                <person name="Leys N."/>
                <person name="Mergeay M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08223.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08432.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516105.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1340"/>
    <dbReference type="EnsemblBacteria" id="ABF08223">
        <property type="protein sequence ID" value="ABF08223"/>
        <property type="gene ID" value="Rmet_1340"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF08432">
        <property type="protein sequence ID" value="ABF08432"/>
        <property type="gene ID" value="Rmet_1549"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1340"/>
    <dbReference type="KEGG" id="rme:Rmet_1549"/>
    <dbReference type="eggNOG" id="COG4962">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_117977_0_0_4"/>
    <dbReference type="OMA" id="FIQHEHA"/>
    <dbReference type="OrthoDB" id="1982724at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1220.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013321">
        <property type="entry name" value="Arc_rbn_hlx_hlx"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010985">
        <property type="entry name" value="Ribbon_hlx_hlx"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47598">
        <property type="entry name" value="SSF47598"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF08223.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF08432.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="154" mass="17381" checksum="ED39964F22CBF53B" modified="2006-05-30" version="1">MSHYRLNLFIQPEHAKRLDELAAKKGVSKSSIVAAALASWLSPDAADQREAAIAKRLDRLSRQSERMERDQNIAIETLALFIRYYLTVSTPVPEAHQEAARAQGKARFEQFTEQLGRQLLRGRSLVRDVVEELHPDPMRMEDAAAATQAQERAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="110" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LNF9</accession>
    <name>Q1LNF9_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 3">Methionine aminopeptidase</fullName>
            <shortName evidence="2">MAP</shortName>
            <shortName evidence="2">MetAP</shortName>
            <ecNumber evidence="2 3">3.4.11.18</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">Peptidase M</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="2 5" type="primary">map</name>
        <name evidence="5" type="ordered locus">Rmet_1434</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 2">Removes the N-terminal methionine from nascent proteins. The N-terminal methionine is often cleaved when the second residue in the primary sequence is small and uncharged (Met-Ala-, Cys, Gly, Pro, Ser, Thr, or Val). Requires deformylation of the N(alpha)-formylated initiator methionine before it can be hydrolyzed.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2 3">
            <text>Release of N-terminal amino acids, preferentially methionine, from peptides and arylamides.</text>
            <dbReference type="EC" id="3.4.11.18"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 3">
            <name>Co(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:48828"/>
        </cofactor>
        <cofactor evidence="2 3">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <cofactor evidence="2 3">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <cofactor evidence="2 3">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
        <text evidence="2 3">Binds 2 divalent metal cations per subunit. Has a high-affinity and a low affinity metal-binding site. The true nature of the physiological cofactor is under debate. The enzyme is active with cobalt, zinc, manganese or divalent iron ions. Most likely, methionine aminopeptidases function as mononuclear Fe(2+)-metalloproteases under physiological conditions, and the catalytically relevant metal-binding site has been assigned to the histidine-containing high-affinity site.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Monomer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase M24A family. Methionine aminopeptidase type 1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.4.11.18" evidence="2 3"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08317.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516185.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1434"/>
    <dbReference type="MEROPS" id="M24.001"/>
    <dbReference type="EnsemblBacteria" id="ABF08317">
        <property type="protein sequence ID" value="ABF08317"/>
        <property type="gene ID" value="Rmet_1434"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1434"/>
    <dbReference type="eggNOG" id="COG0024">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015857_0_1_4"/>
    <dbReference type="OMA" id="GDHAYTF"/>
    <dbReference type="OrthoDB" id="1285136at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070006">
        <property type="term" value="F:metalloaminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046914">
        <property type="term" value="F:transition metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070084">
        <property type="term" value="P:protein initiator methionine removal"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01086">
        <property type="entry name" value="MetAP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01974">
        <property type="entry name" value="MetAP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036005">
        <property type="entry name" value="Creatinase/aminopeptidase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000994">
        <property type="entry name" value="Pept_M24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001714">
        <property type="entry name" value="Pept_M24_MAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002467">
        <property type="entry name" value="Pept_M24A_MAP1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00557">
        <property type="entry name" value="Peptidase_M24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00599">
        <property type="entry name" value="MAPEPTIDASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55920">
        <property type="entry name" value="SSF55920"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00500">
        <property type="entry name" value="met_pdase_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00680">
        <property type="entry name" value="MAP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3 5" id="KW-0031">Aminopeptidase</keyword>
    <keyword evidence="2 5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2 3" id="KW-0645">Protease</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase_M24" evidence="4">
        <location>
            <begin position="13"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="84"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="101"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="113"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="176"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="183"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="209"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="240"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002521"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003653"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABF08317.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="272" mass="29710" checksum="F928066722C35260" modified="2006-05-30" version="1">MSIHINTAEDIAQMRVACRLASEVLDYITPHVKPGVTTGELDRLCHAYMRDVQGTVPAPLNYAPPGYPPFPGAICTSVNDVICHGIPGDRVLKSGDVVNLDITVITKEGYYGDTSRMFVVGEASILAKRLSQITYECMWKGIQQVRNGARLGDIGHAIQTHAEAAGYSVVREYCGHGIGKVFHSDPQVLHYGRPATGMEIKAGMIFTVEPMINAGKRDIRTMPDQWTVKTRDRSLSAQWEHTVLVTETGYEVLTLSEGSPLPPAFITETVAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="60" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LNH9</accession>
    <name>Q1LNH9_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_1414</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08297.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516169.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1414"/>
    <dbReference type="EnsemblBacteria" id="ABF08297">
        <property type="protein sequence ID" value="ABF08297"/>
        <property type="gene ID" value="Rmet_1414"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1414"/>
    <dbReference type="eggNOG" id="COG2866">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_055736_0_0_4"/>
    <dbReference type="OMA" id="NPYGFSH"/>
    <dbReference type="OrthoDB" id="1584387at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021259">
        <property type="entry name" value="DUF2817"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10994">
        <property type="entry name" value="DUF2817"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF08297.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="361" mass="39216" checksum="D794C7F0FC4540BE" modified="2006-05-30" version="1">MPASQTARYFARSYAEARQKFLAAARACGAALTEFPHPTETGNEGEALAMDVAWVGAADASRVLLVTSGTHGAEGFCGSGTQVAMLADTGLHSDCHRAGCALMFIHAVNPYGFSHLRRVNEDNVDLNRNFADFERPLPANPAYAEIAPMLLPSQWPPSCDEHTALMKRVTERGMGWFQAAISAGQYQDPDGMFFGGQQATWSNYTLRRILARFGAGRTTLRWVDVHTGLGPWGYGEAIYMGPDEARQLNKTRAIWGGSVTSIYDGSSTSANLTGLAWAAVPQTLPTIDYAGIALEFGTLPLPEVLDALRGDHWLHVHPEADENQRALIKQAVWRAFYGDSDEWRDGVVAQVTDAVRKGIGA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-02-05" modified="2022-05-25" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LNM2</accession>
    <name>SSUD_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Alkanesulfonate monooxygenase</fullName>
            <ecNumber evidence="1">1.14.14.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">FMNH2-dependent aliphatic sulfonate monooxygenase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">ssuD</name>
        <name type="ordered locus">Rmet_1371</name>
    </gene>
    <organism>
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the desulfonation of aliphatic sulfonates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>an alkanesulfonate + FMNH2 + O2 = an aldehyde + FMN + 2 H(+) + H2O + sulfite</text>
            <dbReference type="Rhea" id="RHEA:23064"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:17359"/>
            <dbReference type="ChEBI" id="CHEBI:17478"/>
            <dbReference type="ChEBI" id="CHEBI:57618"/>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
            <dbReference type="ChEBI" id="CHEBI:134249"/>
            <dbReference type="EC" id="1.14.14.5"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the SsuD family.</text>
    </comment>
    <dbReference type="EC" id="1.14.14.5" evidence="1"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08254.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516133.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q1LNM2"/>
    <dbReference type="SMR" id="Q1LNM2"/>
    <dbReference type="STRING" id="266264.Rmet_1371"/>
    <dbReference type="EnsemblBacteria" id="ABF08254">
        <property type="protein sequence ID" value="ABF08254"/>
        <property type="gene ID" value="Rmet_1371"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1371"/>
    <dbReference type="eggNOG" id="COG2141">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027853_1_0_4"/>
    <dbReference type="OMA" id="NIFWFLP"/>
    <dbReference type="OrthoDB" id="919913at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008726">
        <property type="term" value="F:alkanesulfonate monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01229">
        <property type="entry name" value="Alkanesulf_monooxygen"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019911">
        <property type="entry name" value="Alkanesulphonate_mOase_FMN-dep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011251">
        <property type="entry name" value="Luciferase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036661">
        <property type="entry name" value="Luciferase-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00296">
        <property type="entry name" value="Bac_luciferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51679">
        <property type="entry name" value="SSF51679"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03565">
        <property type="entry name" value="alk_sulf_monoox"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0285">Flavoprotein</keyword>
    <keyword id="KW-0288">FMN</keyword>
    <keyword id="KW-0503">Monooxygenase</keyword>
    <keyword id="KW-0560">Oxidoreductase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_1000066834" description="Alkanesulfonate monooxygenase">
        <location>
            <begin position="1"/>
            <end position="387"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01229"/>
        </source>
    </evidence>
    <sequence length="387" mass="41844" checksum="E8B67249071A3DCC" modified="2006-05-30" version="1">MQVLWFIPTHGDSRYLGTSEGAREVSFDYLKQVAVAADTLGYDGVLIPTGRSCEDPWVVASALAAVTRKLRFLVALRPGLMTPTLAARMAATFDRVSNGRLLVNLVTGGDVAELEGDGLFLNHAERYEASAEFIRVWRDLLAASHENGEISFEGKHVTVKGARVLYPPIQRPHPPVYFGGSSEAAHDLAAEQVETYLTWGEPPADVAKKIADVRARAAKHGRTVRFGIRLHVIVRETDAAAWAAADELISKLDDQTVARAQAVFAKMDSEGQRRMAALHAGGTRRTREALEISPNLWAGVGLVRGGAGTALVGDPKTVAARIEEYAALGIDTFVLSGYPHLEEAYRFAELVFPLLPRKVRDKLPGQVLSGPFGEVMATGIVPIASQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="70" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LNR4</accession>
    <name>Q1LNR4_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Phenol hydroxylase P2 protein (Phenol 2-monooxygenase P2 component) (Protein belongs to CMGI-2)</fullName>
            <ecNumber evidence="2">1.14.13.7</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">tomA2</name>
        <name evidence="2" type="ordered locus">Rmet_1329</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TmoD/XamoD family.</text>
    </comment>
    <dbReference type="EC" id="1.14.13.7" evidence="2"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08212.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516095.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1329"/>
    <dbReference type="EnsemblBacteria" id="ABF08212">
        <property type="protein sequence ID" value="ABF08212"/>
        <property type="gene ID" value="Rmet_1329"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1329"/>
    <dbReference type="eggNOG" id="ENOG5032S7J">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_148539_2_0_4"/>
    <dbReference type="OMA" id="KIDAPGH"/>
    <dbReference type="OrthoDB" id="1929261at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018662">
        <property type="term" value="F:phenol 2-monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006725">
        <property type="term" value="P:cellular aromatic compound metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.56.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003454">
        <property type="entry name" value="MOase_MmoB_DmpM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036889">
        <property type="entry name" value="mOase_MmoB_DmpM_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02406">
        <property type="entry name" value="MmoB_DmpM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56029">
        <property type="entry name" value="SSF56029"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006313"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF08212.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="89" mass="10014" checksum="8D01744400395742" modified="2006-05-30" version="1">MSNVFIAFQANEESRSVVEAILADNPNAVATESPGMVKIDAPGHLTINRRSIEDRIGMKFDLQQIHINLITLSGYIDEDDEQFTLSWKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="63" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LP07</accession>
    <name>Q1LP07_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_1233</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08119.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516007.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1233"/>
    <dbReference type="EnsemblBacteria" id="ABF08119">
        <property type="protein sequence ID" value="ABF08119"/>
        <property type="gene ID" value="Rmet_1233"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1233"/>
    <dbReference type="eggNOG" id="COG3064">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_064668_0_0_4"/>
    <dbReference type="OMA" id="CIDKAKD"/>
    <dbReference type="OrthoDB" id="1514869at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="141"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="141"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="228"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="244"/>
            <end position="258"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF08119.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="258" mass="28629" checksum="528FB9812D2A0A39" modified="2006-05-30" version="1">MIVKPHPATMAAPVSQVPTVSSTALTIALAALLLAAPFRLAMAQASTPAAAAVPPAPGVARDFDAERKAIDDSRAWAKYRYAVAERACYSKFLVNNCIDKAKEVQRGELQVLRERDLEVGDAERAYRATQRDHDQALRRAEYEASLPKRAADEQASREAFERKQQDQALRDAQHNAGAPQRAANAEAYQKKQDDFATKMQEAQQKGAEQARQHEENAKAYQQKQVDAAQRQKELEERRARAAEKQNQGQSSKPSPFGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LP11</accession>
    <name>Q1LP11_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="9">ABC-type branched-chain amino acid transport systems, ATPase component</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">livG6</name>
        <name evidence="9" type="ordered locus">Rmet_1229</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08115.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516003.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1229"/>
    <dbReference type="EnsemblBacteria" id="ABF08115">
        <property type="protein sequence ID" value="ABF08115"/>
        <property type="gene ID" value="Rmet_1229"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1229"/>
    <dbReference type="eggNOG" id="COG0411">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_2_4"/>
    <dbReference type="OMA" id="EVAMCLA"/>
    <dbReference type="OrthoDB" id="1179151at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032823">
        <property type="entry name" value="BCA_ABC_TP_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12399">
        <property type="entry name" value="BCA_ABC_TP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="1" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-1278">Translocase</keyword>
    <feature type="domain" description="ABC transporter" evidence="8">
        <location>
            <begin position="10"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="42"/>
            <end position="49"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ABF08115.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="255" mass="27610" checksum="CC951CBC9EB0C6CB" modified="2006-05-30" version="1">MSTIPSVPVLDAQSLTRRFGGLTAVNSVDLALHLHEIHAVIGTNGAGKSTLINLLSGELPPSEGRLQLQGRDVTGWVQPKLARHGIGRSYQRNNIFLPLTVRENCRLAAQSRAQRAWRLWEPAQGCRTSRALADEALERAGLMQHAGRLASDLAHGQKRQLEVAMCLATEPVALLLDEPLAGMGAEESERMLDLLRGLKEGHAILLVEHDMEAVFAVADRITVMVNGTVIATGDPASIRANREVQLAYLGEEEAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LP56</accession>
    <name>Q1LP56_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Extra-cytoplasmic Solute Receptor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">bug</name>
        <name evidence="3" type="ordered locus">Rmet_1184</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Periplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the UPF0065 (bug) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08070.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1184"/>
    <dbReference type="EnsemblBacteria" id="ABF08070">
        <property type="protein sequence ID" value="ABF08070"/>
        <property type="gene ID" value="Rmet_1184"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1184"/>
    <dbReference type="eggNOG" id="COG3181">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045683_0_0_4"/>
    <dbReference type="OMA" id="TDMVHIP"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042597">
        <property type="term" value="C:periplasmic space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005064">
        <property type="entry name" value="BUG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042100">
        <property type="entry name" value="Bug_dom1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42928">
        <property type="entry name" value="PTHR42928"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03401">
        <property type="entry name" value="TctC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017082">
        <property type="entry name" value="YflP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0675">Receptor</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF08070.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="356" mass="37655" checksum="3D1B50A0BCE0BD5C" modified="2006-05-30" version="1">MPVDAHHGTKFRIAEPAQLATRTSNTWRHLLKAVLNTGAIAALAVLGLTQTGIAHAQAYPTKPIRLIVPFAAGGTTDIVGRAVSDAIGRELGQPVVVENRGGGGGAIGADALAKSAPDGYTLGIATVSTMATNPATNPKNPYDPIKDFAPITNLVNVPNVLTVNPKVPAKNLKEFIALVKSNPGKYSYASAGKGSISHLDGELFKYITKTDMVHIPYRGSGPALNDTLAGQVNAQFDNLPSSMPFIQSDKLRALAVAAPRRVEGLPNVPTFAEEGMKDMNNMAWYGLVAPAGTPAAIITKVHDAAVKALQDPNVKKRLHDAGAYPDGNTPQQYAAQIKRELELRKKIAVDQNITLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-10-07" version="61" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LP62</accession>
    <name>Q1LP62_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_1178</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08064.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1178"/>
    <dbReference type="EnsemblBacteria" id="ABF08064">
        <property type="protein sequence ID" value="ABF08064"/>
        <property type="gene ID" value="Rmet_1178"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1178"/>
    <dbReference type="HOGENOM" id="CLU_125460_0_0_4"/>
    <dbReference type="OMA" id="DIWESGA"/>
    <dbReference type="OrthoDB" id="1960995at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="64"/>
            <end position="86"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF08064.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="185" mass="20410" checksum="1A72531476CB8C5C" modified="2006-05-30" version="1">MYSISTLSTHCTCRILIFMEDRVRDAGLASTSHWCNLKRNEAPDIRPQTEVPMLQRDAQFTSRICWFAAGLLAGALAASLCAALGMRRARQVADDDDAATAQDPRAMVRASISPESGSDMFEYRGFVVHLFWQALGRERYKAWCDIWEAGAVVQEAGGPPATYPTAAEARMAAGAWARHWVQNNG</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="102" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LPB5</accession>
    <name>Q1LPB5_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Sulfur carrier protein FdhD</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3 4" type="primary">fdhD</name>
        <name evidence="4" type="ordered locus">Rmet_1125</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Required for formate dehydrogenase (FDH) activity. Acts as a sulfur carrier protein that transfers sulfur from IscS to the molybdenum cofactor prior to its insertion into FDH.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the FdhD family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08011.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1125"/>
    <dbReference type="EnsemblBacteria" id="ABF08011">
        <property type="protein sequence ID" value="ABF08011"/>
        <property type="gene ID" value="Rmet_1125"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1125"/>
    <dbReference type="eggNOG" id="COG1526">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_056887_2_0_4"/>
    <dbReference type="OMA" id="WAALFDF"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097163">
        <property type="term" value="F:sulfur carrier activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016783">
        <property type="term" value="F:sulfurtransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006777">
        <property type="term" value="P:Mo-molybdopterin cofactor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00187">
        <property type="entry name" value="FdhD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016193">
        <property type="entry name" value="Cytidine_deaminase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003786">
        <property type="entry name" value="FdhD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30592">
        <property type="entry name" value="PTHR30592"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02634">
        <property type="entry name" value="FdhD-NarQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015626">
        <property type="entry name" value="FdhD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53927">
        <property type="entry name" value="SSF53927"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00129">
        <property type="entry name" value="fdhD_narQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 3" id="KW-0501">Molybdenum cofactor biosynthesis</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Cysteine persulfide intermediate" evidence="3">
        <location>
            <position position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF08011.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="290" mass="31301" checksum="A5DC463164070EE2" modified="2006-05-30" version="1">MVEDEYGQAGYVEREIRRHTGRHTGGHTVPEIDDVAEEVPVALVYNGISHAVMMATPLDLEAFAIGFSLTEGIVDSVAEIRDIEIEPGCDGIEVRIEIGQRAFMALRAHRRTLAGRTGCGVCGTESLALLDLEPTPVGVPARRIEPTPAMLRRATDALQARQVLMAATGGVHAAAWCDAQGDVVAVFEDVGRHNALDKLIGHLTRTRTTFGDGFVLLSSRASYELVRKTARMRIQMLATISAPTSLAIRIAAQAGLRLLSFCRKDGFVEYTAPAENEGFVLRSEPLAVPG</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="113" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LPD7</accession>
    <name>Q1LPD7_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Dihydroorotase</fullName>
            <ecNumber evidence="9">3.5.2.3</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">allB</name>
        <name evidence="9" type="ordered locus">Rmet_1103</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Catalyzes the reversible cyclization of carbamoyl aspartate to dihydroorotate.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the metallo-dependent hydrolases superfamily. DHOase family. Class I DHOase subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.5.2.3" evidence="9"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07989.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515890.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1103"/>
    <dbReference type="EnsemblBacteria" id="ABF07989">
        <property type="protein sequence ID" value="ABF07989"/>
        <property type="gene ID" value="Rmet_1103"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1103"/>
    <dbReference type="eggNOG" id="COG0044">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015572_1_1_4"/>
    <dbReference type="OMA" id="WVTAEVT"/>
    <dbReference type="OrthoDB" id="906155at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004151">
        <property type="term" value="F:dihydroorotase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006221">
        <property type="term" value="P:pyrimidine nucleotide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006680">
        <property type="entry name" value="Amidohydro-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002195">
        <property type="entry name" value="Dihydroorotase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011059">
        <property type="entry name" value="Metal-dep_hydrolase_composite"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01979">
        <property type="entry name" value="Amidohydro_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51338">
        <property type="entry name" value="SSF51338"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="SSF51556"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00483">
        <property type="entry name" value="DIHYDROOROTASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0665">Pyrimidine biosynthesis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Amidohydro-rel" evidence="8">
        <location>
            <begin position="68"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="317"/>
            <end position="339"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002368"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ABF07989.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="465" mass="50256" checksum="9056FC9B1BCC777B" modified="2006-05-30" version="1">MTDGTVPGVGLPSARRHADLLVIGGALVTPNGVERADVACAAGRIVALGDLQCSWTADATLDAKGLHVLPGVIDSQVHFREPGLTHKETLEAGTRGAALGGVTAVFEMPNTQPLTLRAEDLRAKLDAAQGRAWCDHAFYIGGSSVNAEHLEYLESLPGCAGVKIFMGSSFGDLLADDDEVLCRILAHGRRRMAVHAEDEARLRERRIIAESSGDVRQHPVWRDARSALLATQRIVKLAGDTGRRLHILHVSTAEEIAFLADRKDRVTVEVTPHHLTLEAPECYERLGTLAQMNPPVRERCHRNALWQAIREGVGDVIGSDHAPHTPKEKSRPYPASPSGMTGVQTLVPVMLDHVHRGQLSLQRFVDLTSAGPARIFGVEGKGRIALGYDADLTLVDLAARRTIRNDWIASVSGWTPYDGIGVTGWPIHTVVRGHIVVRDEALVGTTPLGRPLRFLEAPPRVPAGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="108" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LPE7</accession>
    <name>Q1LPE7_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Alcohol dehydrogenase, 1-propanol preferring</fullName>
            <ecNumber evidence="4">1.1.1.1</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">adh</name>
        <name evidence="4" type="ordered locus">Rmet_1093</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the zinc-containing alcohol dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.1" evidence="4"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07979.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515880.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1093"/>
    <dbReference type="EnsemblBacteria" id="ABF07979">
        <property type="protein sequence ID" value="ABF07979"/>
        <property type="gene ID" value="Rmet_1093"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1093"/>
    <dbReference type="eggNOG" id="COG1064">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026673_20_1_4"/>
    <dbReference type="OMA" id="GLKMTDT"/>
    <dbReference type="OrthoDB" id="969875at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004022">
        <property type="term" value="F:alcohol dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013149">
        <property type="entry name" value="ADH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013154">
        <property type="entry name" value="ADH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002328">
        <property type="entry name" value="ADH_Zn_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020843">
        <property type="entry name" value="PKS_ER"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08240">
        <property type="entry name" value="ADH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00107">
        <property type="entry name" value="ADH_zinc_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00829">
        <property type="entry name" value="PKS_ER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="SSF50129"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00059">
        <property type="entry name" value="ADH_ZINC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1 4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="PKS_ER" evidence="3">
        <location>
            <begin position="14"/>
            <end position="338"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU361277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF07979.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="342" mass="36061" checksum="6CF70DF21409973C" modified="2006-05-30" version="1">MPAMMKAAVVRAFGAPLTIDEVPVPQPGPGQIQVKIEASGVCHTDLHAAQGDWPVKPSLPFIPGHEGVGFVSAVGANVKRVKEGDRVGVPWLYSACGYCEHCLQGWETLCEKQQNTGYSVNGGYGEFVVADPNYVGLLPKNVDFIEIAPILCAGVTVYKGLKVTDTRPGQWVVISGIGGLGHVAVQYARAMGLRVAAVDIDDAKLAFARKLGAEVAINARTTDPAAFLQKEIGGAHGVLVTAVSPQAFSQAIGMVRRGGTISLNGLPPGDFPTPIFDVVLKAITIRGSIVGTRSDLQEALEFAASGVVKATVSTEKLDSINSIFDRMKAGQIEGRIVLDFSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LPS5</accession>
    <name>Q1LPS5_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">HTH cro/C1-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_0965</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07851.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008643418.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0965"/>
    <dbReference type="EnsemblBacteria" id="ABF07851">
        <property type="protein sequence ID" value="ABF07851"/>
        <property type="gene ID" value="Rmet_0965"/>
    </dbReference>
    <dbReference type="GeneID" id="60825452"/>
    <dbReference type="KEGG" id="rme:Rmet_0965"/>
    <dbReference type="eggNOG" id="COG1396">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_066192_17_5_4"/>
    <dbReference type="OMA" id="SSHCADA"/>
    <dbReference type="OrthoDB" id="2063007at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00093">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001387">
        <property type="entry name" value="Cro/C1-type_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010982">
        <property type="entry name" value="Lambda_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01381">
        <property type="entry name" value="HTH_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00530">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47413">
        <property type="entry name" value="SSF47413"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50943">
        <property type="entry name" value="HTH_CROC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH cro/C1-type" evidence="1">
        <location>
            <begin position="19"/>
            <end position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF07851.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="131" mass="14409" checksum="9C926571BEC3B303" modified="2006-05-30" version="1">MHLMTGDPLKLFGRHLTWLRKQRGWSQEALSLESGLARSYLSGIERGTRNVALYNICTLADTLGVAPSVMLDFSSHCADAPVEPARAPFNPEISPAVQATLRYMTELSDTDRDVVAGVARALARKQLRKIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LQD2</accession>
    <name>Q1LQD2_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Alkylhydroperoxidase AhpD core:Uncharacterized peroxidase-related protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_0758</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07644.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515599.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0758"/>
    <dbReference type="EnsemblBacteria" id="ABF07644">
        <property type="protein sequence ID" value="ABF07644"/>
        <property type="gene ID" value="Rmet_0758"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0758"/>
    <dbReference type="eggNOG" id="COG2128">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_082760_4_0_4"/>
    <dbReference type="OMA" id="MRPNDEF"/>
    <dbReference type="OrthoDB" id="1925754at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004601">
        <property type="term" value="F:peroxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051920">
        <property type="term" value="F:peroxiredoxin activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1290.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029032">
        <property type="entry name" value="AhpD-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004675">
        <property type="entry name" value="AhpD_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003779">
        <property type="entry name" value="CMD-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010195">
        <property type="entry name" value="Uncharacterised_peroxidase-rel"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02627">
        <property type="entry name" value="CMD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69118">
        <property type="entry name" value="SSF69118"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00778">
        <property type="entry name" value="ahpD_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01926">
        <property type="entry name" value="peroxid_rel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="2" id="KW-0575">Peroxidase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CMD" evidence="1">
        <location>
            <begin position="45"/>
            <end position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02627"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF07644.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="194" mass="21706" checksum="1F48F673AF55F59A" modified="2006-05-30" version="1">MTQPISRYPVPAIENLPDDIRQRVLEVQEKAGFVPNVFLALAHRPDECRAFFAYHDALMLKETGSLTKGDREMIVVATSGVNQCLYCVVAHGAILRIYEKKPLVADQVAVNYLKADITPRQRAMLDFAMKVCTDSHTIGEADFEALRAHGFDDEDAWDIAGITAFFGLSNRMANTIGMRPNEQFFLMGRVPKAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="72" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LQD4</accession>
    <name>Q1LQD4_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Glycosyltransferase involved in cell wall biogenesis</fullName>
            <ecNumber evidence="3">2.4.1.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_0756</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.4.1.-" evidence="3"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07642.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515597.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0756"/>
    <dbReference type="CAZy" id="GT2">
        <property type="family name" value="Glycosyltransferase Family 2"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF07642">
        <property type="protein sequence ID" value="ABF07642"/>
        <property type="gene ID" value="Rmet_0756"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0756"/>
    <dbReference type="eggNOG" id="COG1216">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_065962_1_0_4"/>
    <dbReference type="OMA" id="YTELWAL"/>
    <dbReference type="OrthoDB" id="1379602at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001173">
        <property type="entry name" value="Glyco_trans_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00535">
        <property type="entry name" value="Glycos_transf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="SSF53448"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glyco_trans_2-like" evidence="2">
        <location>
            <begin position="4"/>
            <end position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF07642.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="260" mass="29079" checksum="8274E6E97470444F" modified="2006-05-30" version="1">MKISVVLITKNEAHNIRECLESVSWCDRAIIVDSGSTDGTVETARAMGAEVHQTATWPGFGPQKNLALSKAQSEWVLSIDADERVTPELRDEILAAIASGQADAYDMPRLSRFCGRFIRHSGWYPDRLTRLFRVGKARFTDDLVHENVVTDGPIAHLRNPLLHYTYDDFSQVLRKVDQYSTLGATQAFQRGKTATPASAWLHGSWAFLRTYLLRRGFLDGPQGLAIALMNGQASYYKYIKLWLLQQSARQPAASGDPQAH</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LQE8</accession>
    <name>Q1LQE8_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">DNA repair photolyase</fullName>
            <ecNumber evidence="8">4.1.99.3</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">splB</name>
        <name evidence="8" type="ordered locus">Rmet_0742</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EC" id="4.1.99.3" evidence="8"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07628.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515583.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0742"/>
    <dbReference type="EnsemblBacteria" id="ABF07628">
        <property type="protein sequence ID" value="ABF07628"/>
        <property type="gene ID" value="Rmet_0742"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0742"/>
    <dbReference type="eggNOG" id="COG1533">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015525_0_0_4"/>
    <dbReference type="OMA" id="GNTDCYQ"/>
    <dbReference type="OrthoDB" id="979208at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003904">
        <property type="term" value="F:deoxyribodipyrimidine photo-lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaB/NifB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040086">
        <property type="entry name" value="MJ0683-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43432">
        <property type="entry name" value="PTHR43432"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01084">
        <property type="entry name" value="Uncharacterised_Radical_SAM_Su"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="4" id="KW-0408">Iron</keyword>
    <keyword evidence="5" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="8" id="KW-0456">Lyase</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <feature type="domain" description="Elp3" evidence="7">
        <location>
            <begin position="70"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ABF07628.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="361" mass="40292" checksum="C904D4578F7CDAA1" modified="2006-05-30" version="1">MTSLPDGFRKGRGALSNTESRFDSLSREADAAMLADLEALHGESPAPRTTIAIEPARTIISRNQSPDVPFDQSINMYRGCEHGCSYCFARPNHAYLGLSPGLDFETKLFAKPNAATLLEQELRKKHYKPSVIALGTNTDPYQPIERKFQLTRGILEVLERFQHPVAITTKSFLVSRDIDILARMARNGLARVFMSVTTLDADIARKMEPRASTPAKRIEGIRLLAEAGVPAGVLFAPVIPALTDYDLENVLKACSEVGATSAGFVMLRLPLEVHGIFIEWLEEHFPGRKQHVLSIIENMRNGKHNDATFTSRMRGTGIFAELVRKRFEIACRRLGLNQERHPLRTDLFRVPPEDSPQGSLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="99" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LQG4</accession>
    <name>Q1LQG4_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 7">UDP-glucose 6-dehydrogenase</fullName>
            <ecNumber evidence="4 7">1.1.1.22</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">ugd</name>
        <name evidence="12" type="ordered locus">Rmet_0726</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 7">
            <text>H2O + 2 NAD(+) + UDP-alpha-D-glucose = 3 H(+) + 2 NADH + UDP-alpha-D-glucuronate</text>
            <dbReference type="Rhea" id="RHEA:23596"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:58052"/>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
            <dbReference type="EC" id="1.1.1.22"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Nucleotide-sugar biosynthesis; UDP-alpha-D-glucuronate biosynthesis; UDP-alpha-D-glucuronate from UDP-alpha-D-glucose: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 7">Belongs to the UDP-glucose/GDP-mannose dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.22" evidence="4 7"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07612.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515573.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0726"/>
    <dbReference type="EnsemblBacteria" id="ABF07612">
        <property type="protein sequence ID" value="ABF07612"/>
        <property type="gene ID" value="Rmet_0726"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0726"/>
    <dbReference type="eggNOG" id="COG1004">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023810_1_2_4"/>
    <dbReference type="OMA" id="CECLNLP"/>
    <dbReference type="OrthoDB" id="647136at2"/>
    <dbReference type="UniPathway" id="UPA00038">
        <property type="reaction ID" value="UER00491"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003979">
        <property type="term" value="F:UDP-glucose 6-dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000271">
        <property type="term" value="P:polysaccharide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006065">
        <property type="term" value="P:UDP-glucuronate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008927">
        <property type="entry name" value="6-PGluconate_DH-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017476">
        <property type="entry name" value="UDP-Glc/GDP-Man"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014027">
        <property type="entry name" value="UDP-Glc/GDP-Man_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036220">
        <property type="entry name" value="UDP-Glc/GDP-Man_DH_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014026">
        <property type="entry name" value="UDP-Glc/GDP-Man_DH_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001732">
        <property type="entry name" value="UDP-Glc/GDP-Man_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028357">
        <property type="entry name" value="UDPglc_DH_bac"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00984">
        <property type="entry name" value="UDPG_MGDP_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03720">
        <property type="entry name" value="UDPG_MGDP_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03721">
        <property type="entry name" value="UDPG_MGDP_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF500134">
        <property type="entry name" value="UDPglc_DH_bac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000124">
        <property type="entry name" value="UDPglc_GDPman_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00984">
        <property type="entry name" value="UDPG_MGDP_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48179">
        <property type="entry name" value="SSF48179"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52413">
        <property type="entry name" value="SSF52413"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03026">
        <property type="entry name" value="NDP-sugDHase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7 10" id="KW-0520">NAD</keyword>
    <keyword evidence="5 7 12" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UDPG_MGDP_dh_C" evidence="11">
        <location>
            <begin position="325"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="9">
        <location>
            <begin position="156"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="9">
        <location>
            <begin position="260"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="8">
        <location>
            <position position="271"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="30"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="35"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="86"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="121"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="159"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="215"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="268"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="274"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="332"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="339"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000874"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR000124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR500134-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR500134-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR500134-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ABF07612.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="457" mass="49777" checksum="903C4714E3D49FE7" modified="2006-05-30" version="1">MKVTIIGSGYVGLVTGACLAELGNSVFCLDVDEKKIALLNAGGVPIYEPGLKELIDRNRAAGRLTFSTDVAASVEHADVQFIAVGTPPDEDGSADLKYVLAAARNIGRHMTGFKVVVDKSTVPVGTGDRVSAVIREELAARGLEDLQFSVVSNPEFLKEGAAVEDFMRPDRIVLGCAPDVAGRHAQAIMRQLYSPFNRNHERTFYMDVRSAEFTKYAANSMLATRISFMNELANLADEVGADIELVRLGIGSDPRIGYSFLYAGAGYGGSCFPKDVQALMRTASAHGKPMRVLEAVEAVNDSQKQVLGDKIIKRFGEDLTGKTFGVWGLAFKPNTDDMREAPSRVLAREMISRGASLRVHDPVAMDEARRVLELDLADIPDAMARIQFCNAQMDVLKDADALVIVTEWKVFRSPDFSQIRNMLKTPVIIDGRNLYEPDAMAEAGIEYHAIGRSSSAH</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-12-02" version="57" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LQR8</accession>
    <name>Q1LQR8_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_0622</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07508.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0622"/>
    <dbReference type="EnsemblBacteria" id="ABF07508">
        <property type="protein sequence ID" value="ABF07508"/>
        <property type="gene ID" value="Rmet_0622"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0622"/>
    <dbReference type="eggNOG" id="COG3522">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_079549_0_0_4"/>
    <dbReference type="OMA" id="VHPWQVY"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010263">
        <property type="entry name" value="T6SS_TssK"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35566">
        <property type="entry name" value="PTHR35566"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05936">
        <property type="entry name" value="T6SS_VasE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03353">
        <property type="entry name" value="VI_chp_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF07508.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="276" mass="30709" checksum="3D7239C2FEB1B183" modified="2006-05-30" version="1">MRTPQGRFELDPVFVPPCLFLSANDRLTERMRRLSEILAAKSASLAVRRRERSDQIADFAVADVALFWLLHSVNGTWPELARLCAAPQQHPERLYAVLSRLAGSLLTFSTTETLQAIPAYDHQTPEPMFAELESLIRTLLDTVIPSRVVPVALEQIRPTVWMGKILDERLVEGAEYYLSVQAGLPAQMLIEQLPRLCKAGAPDEVEQILNSALPGIPLRTMSRLPAAIPVRIENQYFALDSGHPAFQRMLAARACQFYVPASVPDVSLELFAVLPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="97" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LQX0</accession>
    <name>Q1LQX0_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Molybdenum transport system permease</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">modB</name>
        <name evidence="11" type="ordered locus">Rmet_0570</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 9">Part of the binding-protein-dependent transport system for molybdenum; probably responsible for the translocation of the substrate across the membrane.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cell inner membrane</location>
            <topology evidence="9">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="8">Cell membrane</location>
            <topology evidence="8">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the binding-protein-dependent transport system permease family. CysTW subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07456.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008640894.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0570"/>
    <dbReference type="EnsemblBacteria" id="ABF07456">
        <property type="protein sequence ID" value="ABF07456"/>
        <property type="gene ID" value="Rmet_0570"/>
    </dbReference>
    <dbReference type="GeneID" id="60824167"/>
    <dbReference type="KEGG" id="rme:Rmet_0570"/>
    <dbReference type="eggNOG" id="COG4149">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016047_14_3_4"/>
    <dbReference type="OMA" id="MYSFIET"/>
    <dbReference type="OrthoDB" id="1915735at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015098">
        <property type="term" value="F:molybdate ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011867">
        <property type="entry name" value="ModB_ABC"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="SSF161098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02141">
        <property type="entry name" value="modB_ABC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="9" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 9" id="KW-0500">Molybdenum</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="8" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="14"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="46"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="85"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="195"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="10">
        <location>
            <begin position="8"/>
            <end position="211"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022505"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU365097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ABF07456.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="238" mass="25221" checksum="C9B70ADCCBB3DED7" modified="2006-05-30" version="1">MDAVWVPLLLSLKVAGWATLLNAILGVAAAYAISRWRSSARDVVDAILTLPLVLPPTVLGYYLLVLVGRRGVFGAWLDRIGIELVFTWQGAVLASTVVAFPLVLKSARAAFEGVDHQLENAARVLGVSETGIFFRVTLPMALRGISAGVVLAFARALGEFGATLMVAGNLPGRTQTLSVAIYEAVQAGDDNTANLLVLVTSVTCIVLLVVAGRLVPPAARGVGEVYERRAFRREAVGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="100" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LRE2</accession>
    <name>Q1LRE2_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Glutamate dehydrogenase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">gdhA</name>
        <name evidence="11" type="ordered locus">Rmet_0398</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Catalyzes the reversible oxidative deamination of glutamate to alpha-ketoglutarate and ammonia.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>H2O + L-glutamate + NADP(+) = 2-oxoglutarate + H(+) + NADPH + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:11612"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.4.1.4"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="3 5 9">Belongs to the Glu/Leu/Phe/Val dehydrogenases family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07284.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515276.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0398"/>
    <dbReference type="EnsemblBacteria" id="ABF07284">
        <property type="protein sequence ID" value="ABF07284"/>
        <property type="gene ID" value="Rmet_0398"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0398"/>
    <dbReference type="eggNOG" id="COG0334">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025763_1_2_4"/>
    <dbReference type="OMA" id="WLQNRNG"/>
    <dbReference type="OrthoDB" id="1184827at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004354">
        <property type="term" value="F:glutamate dehydrogenase (NADP+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006520">
        <property type="term" value="P:cellular amino acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01076">
        <property type="entry name" value="NAD_bind_1_Glu_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046346">
        <property type="entry name" value="Aminiacid_DH-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006095">
        <property type="entry name" value="Glu/Leu/Phe/Val_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033524">
        <property type="entry name" value="Glu/Leu/Phe/Val_DH_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006096">
        <property type="entry name" value="Glu/Leu/Phe/Val_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006097">
        <property type="entry name" value="Glu/Leu/Phe/Val_DH_dimer_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014362">
        <property type="entry name" value="Glu_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033922">
        <property type="entry name" value="NAD_bind_Glu_DH"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00208">
        <property type="entry name" value="ELFV_dehydrog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02812">
        <property type="entry name" value="ELFV_dehydrog_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000185">
        <property type="entry name" value="Glu_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00082">
        <property type="entry name" value="GLFDHDRGNASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00839">
        <property type="entry name" value="ELFV_dehydrog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53223">
        <property type="entry name" value="SSF53223"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00074">
        <property type="entry name" value="GLFV_DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0520">NAD</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4 5 9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ELFV_dehydrog" evidence="10">
        <location>
            <begin position="203"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="6">
        <location>
            <position position="126"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="90"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="114"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="210"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="241"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="368"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Important for catalysis" evidence="8">
        <location>
            <position position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001463"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00003868"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRNR" id="PIRNR000185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR000185-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR000185-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR000185-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU004417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ABF07284.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="435" mass="47255" checksum="CD8BAD91493261FB" modified="2006-05-30" version="1">MSSAAPTQNVSNGHTLPSYLNADNLGPWGIYLQQVDRVTPYLGSLARWVETLKRPKRAMIVDVPIELDNGTIAHFEGYRVQHNLSRGPGKGGVRFHQDVTLSEVMALSAWMSVKNAAVNVPYGGAKGGIRVDPRTLSHGELERLTRRYTSEINIIIGPSKDIPAPDVNTNAQVMAWMMDTYSMNSGSTATGVVTGKPISLGGSLGRHEATGRGVFVVGSEAARNLGMDVKGARVAVQGFGNVGAVAAKLFHEAGATVVAVQDHRTTLFDPAGLDVPKMMEYASHSGTIEGYRAEVISTEQFWEVDCDILIPAALEGQLTASNAPKIKAKLVIEGANGPTTPEADDILRERNILVCPDVIANAGGVTVSYFEWVQDFSSFFWTEEEINERLVRIMQEAFRAIWQVAQDNKVTLRTAAFIVACTRILQAREMRGLYP</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="64" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LRG1</accession>
    <name>Q1LRG1_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_0379</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07265.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515257.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0379"/>
    <dbReference type="EnsemblBacteria" id="ABF07265">
        <property type="protein sequence ID" value="ABF07265"/>
        <property type="gene ID" value="Rmet_0379"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0379"/>
    <dbReference type="eggNOG" id="ENOG5033RGH">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_917406_0_0_4"/>
    <dbReference type="OMA" id="RFLIQKY"/>
    <dbReference type="OrthoDB" id="1266878at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="162"/>
            <end position="189"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF07265.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="311" mass="33761" checksum="8E2F327C542AAF38" modified="2006-05-30" version="1">MNLDTELIRAFLQVHAETILTWLAAGLLMMLLARFELRRAVRKSAQAMSQSVAATVAACVPDIAHAAQQGHTVPGELAATGTEPVATQRADALRKLALDTVATVMTRGRWLEDVGNLRLGEDALLSGRRAGGADPLLVVAPQPVVQAYLNAQRTFDDEATTLQNHRREALRLQEALQALNAEAAHVEQETASIVMRFEQVNAQVAQGVEAGDYQRFLIQKYNQLGVREKEIGQERASMAAQAREAADALASLAHTASQRYTQALAPLMQQLRSAWGHGDSPEVFDTWLRAAPAAQPGDRQATIRPGEHTLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="61" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LRH0</accession>
    <name>Q1LRH0_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">GBBH-like_N domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Rmet_0370</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07256.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515247.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0370"/>
    <dbReference type="EnsemblBacteria" id="ABF07256">
        <property type="protein sequence ID" value="ABF07256"/>
        <property type="gene ID" value="Rmet_0370"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0370"/>
    <dbReference type="eggNOG" id="COG3536">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_117841_0_1_4"/>
    <dbReference type="OMA" id="TAINLHK"/>
    <dbReference type="OrthoDB" id="2003927at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2020.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010376">
        <property type="entry name" value="GBBH-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038492">
        <property type="entry name" value="GBBH-like_N_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06155">
        <property type="entry name" value="GBBH-like_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0408">Iron</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GBBH-like_N" evidence="3">
        <location>
            <begin position="15"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF06155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF07256.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="138" mass="15129" checksum="BF06D68A29D56B29" modified="2006-05-30" version="1">MAGLEKDTPHPTALTVHTQSRVLEIGFDNGNTWRLPFELLRVYSPSAEVQGHGPGQETLQTGKRDVGIAAVEPVGNYAIQIRFDDGHDTGIYSWELLYRLGDQQEALWADYLKRLADAGLDRDAPMAAAGGHACGHHH</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LRI6</accession>
    <name>Q1LRI6_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">L-threonine dehydratase</fullName>
            <ecNumber evidence="11">4.3.1.19</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Threonine deaminase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11 13" type="primary">ilvA</name>
        <name evidence="13" type="ordered locus">Rmet_0354</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10 11">Catalyzes the anaerobic formation of alpha-ketobutyrate and ammonia from threonine in a two-step reaction. The first step involved a dehydration of threonine and a production of enamine intermediates (aminocrotonate), which tautomerizes to its imine form (iminobutyrate). Both intermediates are unstable and short-lived. The second step is the nonenzymatic hydrolysis of the enamine/imine intermediates to form 2-ketobutyrate and free ammonia. In the low water environment of the cell, the second step is accelerated by RidA.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 11">
            <text>L-threonine = 2-oxobutanoate + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:22108"/>
            <dbReference type="ChEBI" id="CHEBI:16763"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:57926"/>
            <dbReference type="EC" id="4.3.1.19"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 11">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3 11">Amino-acid biosynthesis; L-isoleucine biosynthesis; 2-oxobutanoate from L-threonine: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Homotetramer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 11">Belongs to the serine/threonine dehydratase family.</text>
    </comment>
    <dbReference type="EC" id="4.3.1.19" evidence="11"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07240.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0354"/>
    <dbReference type="EnsemblBacteria" id="ABF07240">
        <property type="protein sequence ID" value="ABF07240"/>
        <property type="gene ID" value="Rmet_0354"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0354"/>
    <dbReference type="eggNOG" id="COG1171">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021152_6_2_4"/>
    <dbReference type="UniPathway" id="UPA00047">
        <property type="reaction ID" value="UER00054"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004794">
        <property type="term" value="F:L-threonine ammonia-lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009097">
        <property type="term" value="P:isoleucine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1020.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1100">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045865">
        <property type="entry name" value="ACT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001926">
        <property type="entry name" value="PLP-dep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000634">
        <property type="entry name" value="Ser/Thr_deHydtase_PyrdxlP-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001721">
        <property type="entry name" value="TD_ACT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038110">
        <property type="entry name" value="TD_ACT-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005787">
        <property type="entry name" value="Thr_deHydtase_biosynth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036052">
        <property type="entry name" value="Trypto_synt_PLP_dependent"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00291">
        <property type="entry name" value="PALP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00585">
        <property type="entry name" value="Thr_dehydrat_C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53686">
        <property type="entry name" value="SSF53686"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55021">
        <property type="entry name" value="SSF55021"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01124">
        <property type="entry name" value="ilvA_2Cterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51672">
        <property type="entry name" value="ACT_LIKE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00165">
        <property type="entry name" value="DEHYDRATASE_SER_THR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="9 11" id="KW-0100">Branched-chain amino acid biosynthesis</keyword>
    <keyword evidence="5 11" id="KW-0412">Isoleucine biosynthesis</keyword>
    <keyword evidence="8 11 13" id="KW-0456">Lyase</keyword>
    <keyword evidence="7 11" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="ACT-like" evidence="12">
        <location>
            <begin position="391"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="domain" description="ACT-like" evidence="12">
        <location>
            <begin position="485"/>
            <end position="556"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004810"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010869"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022624"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00025527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU362012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51672"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ABF07240.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="565" mass="62317" checksum="44FE444FCCDDA244" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="68" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LRV1</accession>
    <name>Q1LRV1_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_0239</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07125.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515137.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0239"/>
    <dbReference type="EnsemblBacteria" id="ABF07125">
        <property type="protein sequence ID" value="ABF07125"/>
        <property type="gene ID" value="Rmet_0239"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0239"/>
    <dbReference type="eggNOG" id="ENOG50318BZ">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_074587_2_0_4"/>
    <dbReference type="OMA" id="YIWRGLT"/>
    <dbReference type="OrthoDB" id="1970612at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010239">
        <property type="entry name" value="CHP02001"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09694">
        <property type="entry name" value="Gcw_chp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02001">
        <property type="entry name" value="gcw_chp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193395" evidence="1">
        <location>
            <begin position="25"/>
            <end position="266"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF07125.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="266" mass="28947" checksum="E0684FCBD8CD0163" modified="2006-05-30" version="1">MKKSSLAVSAAAALFSLATSVAFAQSAPEAAAPAAAPAAEPASPHTITANVSLVSDYRYRGLSQTNRRPAIQGGFDYAHESGFYIGNWNSTISWISDADKSVSAPVEMDFYGGFKNTFKMSDLEFNYDVGVLQYFYPGGYSNPRPYTTELYAGIGYGPVFLKYSQAVTNLFGIANSQYSSYIDLAFNYPLNVWDLTLNAHLGYQNVQHNSAASYLDWKVGLTKDLGKGFALAVAYIGTNAKESFYTNSYNHNVGNNTAWVSLSKTF</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LS12</accession>
    <name>Q1LS12_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Glutathione S-transferase</fullName>
            <ecNumber evidence="3">2.5.1.18</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">gst</name>
        <name evidence="3" type="ordered locus">Rmet_0178</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.5.1.18" evidence="3"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07064.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515087.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0178"/>
    <dbReference type="EnsemblBacteria" id="ABF07064">
        <property type="protein sequence ID" value="ABF07064"/>
        <property type="gene ID" value="Rmet_0178"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0178"/>
    <dbReference type="eggNOG" id="COG0625">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_070658_0_0_4"/>
    <dbReference type="OMA" id="CPMNIEA"/>
    <dbReference type="OrthoDB" id="1512459at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004364">
        <property type="term" value="F:glutathione transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006749">
        <property type="term" value="P:glutathione metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036282">
        <property type="entry name" value="Glutathione-S-Trfase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040079">
        <property type="entry name" value="Glutathione_S-Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004045">
        <property type="entry name" value="Glutathione_S-Trfase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13417">
        <property type="entry name" value="GST_N_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00019">
        <property type="entry name" value="Glutathione_Transferase_(cytos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47616">
        <property type="entry name" value="SSF47616"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="SSF52833"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50404">
        <property type="entry name" value="GST_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="GST N-terminal" evidence="2">
        <location>
            <begin position="1"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="209"/>
            <end position="229"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF07064.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="229" mass="25475" checksum="CEF90663627B9FE3" modified="2006-05-30" version="1">MKLVIGNKNYSSWSLRPWLLLRHAGIAFEEIAVRLFTKEFAAEIARYSPAGKVPALIDGDVTVWDSLSISEYVAERFPEKALWPKDAAARALARSICAEMHSGFGNLRSQMPMNVTAMLPGLGWNVAVQRDIDRIASIWTDLRTRYASEGPFLFGSFTIADAFYAPIVSRFATYGVHLPESAKAYADFVLALPAMQEWAAAAREERDFVPADEPYRTEPDRPDAIIVSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="113" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LS58</accession>
    <name>Q1LS58_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">ATP-dependent protease ATPase subunit HslU</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Unfoldase HslU</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5 8" type="primary">hslU</name>
        <name evidence="8" type="ordered locus">Rmet_0132</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">ATPase subunit of a proteasome-like degradation complex; this subunit has chaperone activity. The binding of ATP and its subsequent hydrolysis by HslU are essential for unfolding of protein substrates subsequently hydrolyzed by HslV. HslU recognizes the N-terminal part of its protein substrates and unfolds these before they are guided to HslV for hydrolysis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">A double ring-shaped homohexamer of HslV is capped on each side by a ring-shaped HslU homohexamer. The assembly of the HslU/HslV complex is dependent on binding of ATP.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the ClpX chaperone family. HslU subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07018.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0132"/>
    <dbReference type="EnsemblBacteria" id="ABF07018">
        <property type="protein sequence ID" value="ABF07018"/>
        <property type="gene ID" value="Rmet_0132"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0132"/>
    <dbReference type="eggNOG" id="COG1220">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033123_0_0_4"/>
    <dbReference type="OMA" id="KYGMIKT"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009376">
        <property type="term" value="C:HslUV protease complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008233">
        <property type="term" value="F:peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036402">
        <property type="term" value="F:proteasome-activating activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043335">
        <property type="term" value="P:protein unfolding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00249">
        <property type="entry name" value="HslU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019489">
        <property type="entry name" value="Clp_ATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004491">
        <property type="entry name" value="HslU"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48102:SF3">
        <property type="entry name" value="PTHR48102:SF3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07724">
        <property type="entry name" value="AAA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10431">
        <property type="entry name" value="ClpB_D2-small"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01086">
        <property type="entry name" value="ClpB_D2-small"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00390">
        <property type="entry name" value="hslU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 5" id="KW-0143">Chaperone</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0645">Protease</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA" evidence="6">
        <location>
            <begin position="46"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="domain" description="ClpB_D2-small" evidence="7">
        <location>
            <begin position="331"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="15"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="57"/>
            <end position="62"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="252"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="317"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="389"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM01086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ABF07018.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="439" mass="49107" checksum="202D9221CD5562E0" modified="2006-05-30" version="1">MTPSEIVSELDKHIIGQNKAKKAVAVALRNRWRRQQVAEPLRQEITPKNILMIGPTGVGKTEIARRLAKLADAPFIKIEATKFTEVGYVGRDVDTIVRDLAEMAVKQTRESEMKKVRTKAEDAAEDRLLDVLLPPPRDIGFAQHEEKDSNTRQVFRKKLREGELDEKDIELEVSAGMPNMDIMGPPGMEDMTEQIRSMFAGLGQGKKARRKMKVKDAFKLLIDEEAAKLVNEEELKHKAIANVEQNGIVFLDEIDKIASRSEVGGGEVSRQGVQRDLLPLVEGTTVNTKYGMIKTDHILFIASGAFHLSKPSDLIPELQGRFPIRVELESLSVQDFEAILTQTDASLTKQYQALLKTEEVELQFSPDGIRRLAEIAFSVNEKVENIGARRLYTVMERLLEDLSFHATKSSGETVTVDAAYVDARLGDLAGNEDLSRYVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-01-04" modified="2021-06-02" version="101" xmlns="http://uniprot.org/uniprot">
    <accession>Q5NV17</accession>
    <name>Q5NV17_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Tyrosine-based site-specific recombinase activity site-specific DNA recombination</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">int</name>
        <name evidence="7" type="ordered locus">Rmet_6252</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="7 8">
        <name>pMOL28</name>
    </geneLocation>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="8">Plasmid pMOL28</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000355">
        <property type="protein sequence ID" value="ABF13111.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011239934.1">
        <property type="nucleotide sequence ID" value="NC_007972.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_161673.1">
        <property type="nucleotide sequence ID" value="NC_006525.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF13111">
        <property type="protein sequence ID" value="ABF13111"/>
        <property type="gene ID" value="Rmet_6252"/>
    </dbReference>
    <dbReference type="GeneID" id="60824667"/>
    <dbReference type="KEGG" id="rme:Rmet_6252"/>
    <dbReference type="HOGENOM" id="CLU_027562_9_6_4"/>
    <dbReference type="OMA" id="VRDRMGH"/>
    <dbReference type="OrthoDB" id="745068at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid pMOL28"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.443.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044068">
        <property type="entry name" value="CB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011010">
        <property type="entry name" value="DNA_brk_join_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013762">
        <property type="entry name" value="Integrase-like_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002104">
        <property type="entry name" value="Integrase_catalytic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010998">
        <property type="entry name" value="Integrase_recombinase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004107">
        <property type="entry name" value="Integrase_SAM-like_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02899">
        <property type="entry name" value="Phage_int_SAM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00589">
        <property type="entry name" value="Phage_integrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56349">
        <property type="entry name" value="SSF56349"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51900">
        <property type="entry name" value="CB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51898">
        <property type="entry name" value="TYR_RECOMBINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0229">DNA integration</keyword>
    <keyword evidence="3" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="2 4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-0614">Plasmid</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Core-binding (CB)" evidence="6">
        <location>
            <begin position="28"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="domain" description="Tyr recombinase" evidence="5">
        <location>
            <begin position="161"/>
            <end position="363"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABF13111.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="381" mass="43615" checksum="9D9B4A8C8C02F242" modified="2005-01-04" version="1">MKLFFTDGQFAVAGYSRPDVPFLCDAEMELVDAPNRYLRYIATVKGRTRAANTWRTYGAALYEFFAFLEANGLAWDRVDQSQIAAWRDTMLERGCARSTVNQRLRTVDAFYNWAKSNGMTHSIPFDRTDVWVAKPRGFLAHVDASGDRFEANSLTVQTHKVTPQFLHLDRAIRFLDALTPYRMRLMGYLALLTGMRREEVVSMDCRVLPNPAGRDSNKQIPMHLDPSITPTKGEKDRTVMLPYDLAVALYQYFTFERSRLLAKHRAKFGIETTLLFLSQTGDPLSVNGLNNAFRRVSQKTGIWCTPHMLRHTFGTYELMRMSRQKTQTQALLWVRDRMGHNSITTTEIYVHAADLVEHDDVDGYHLDICRALAHGNPTSKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-03-23" modified="2021-06-02" version="36" xmlns="http://uniprot.org/uniprot">
    <accession>D3DXZ4</accession>
    <name>D3DXZ4_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_6558</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ADC45164.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517343.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_6558"/>
    <dbReference type="EnsemblBacteria" id="ADC45164">
        <property type="protein sequence ID" value="ADC45164"/>
        <property type="gene ID" value="Rmet_6558"/>
    </dbReference>
    <dbReference type="GeneID" id="60820777"/>
    <dbReference type="KEGG" id="rme:Rmet_6558"/>
    <dbReference type="eggNOG" id="ENOG5032ZFH">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_188971_0_0_4"/>
    <dbReference type="OrthoDB" id="2010021at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021853">
        <property type="entry name" value="DUF3460"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11943">
        <property type="entry name" value="DUF3460"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADC45164.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="63" mass="7301" checksum="600364108AA909C2" modified="2010-03-23" version="1">MAMYESDITQFLKTLKQERPSLDAEQREGRALLWDKAPIDLEERARADASRVAQKPYVYASEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-03-23" modified="2021-06-02" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>D3DY61</accession>
    <name>D3DY61_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_6631</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ADC45231.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518359.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADC45231">
        <property type="protein sequence ID" value="ADC45231"/>
        <property type="gene ID" value="Rmet_6631"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6631"/>
    <dbReference type="HOGENOM" id="CLU_2651868_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="51"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADC45231.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="76" mass="8246" checksum="DEAFA1B883CA9EA4" modified="2010-03-23" version="1">MTVVARIVISGPLNGGWFVTPLHHDGTAGETRRFAAPEEARAFAAATYPGKPIRQANLHPPPMPKGSRVRFPVFPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-03-23" modified="2020-10-07" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>D3DYF9</accession>
    <name>D3DYF9_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_6750</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ADC45329.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_6750"/>
    <dbReference type="EnsemblBacteria" id="ADC45329">
        <property type="protein sequence ID" value="ADC45329"/>
        <property type="gene ID" value="Rmet_6750"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6750"/>
    <dbReference type="HOGENOM" id="CLU_3083915_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADC45329.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="52" mass="5831" checksum="40DCEFD33383B266" modified="2010-03-23" version="1">MWIVYRFGLAAIALIALIALFVCQYRLSARLARREAKLDGQQAHEEPGAIES</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1990-08-01" modified="2022-05-25" version="129" xmlns="http://uniprot.org/uniprot">
    <accession>P17551</accession>
    <accession>Q1L9W5</accession>
    <accession>Q5NV00</accession>
    <name>CHRA1_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Chromate transport protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">chrA1</name>
        <name evidence="4" type="synonym">chrA</name>
        <name evidence="6" type="ordered locus">Rmet_6202</name>
        <name evidence="8" type="ORF">RMe0090</name>
    </gene>
    <organism>
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid">
        <name>pMOL28</name>
    </geneLocation>
    <reference key="1">
        <citation type="journal article" date="1990" name="J. Biol. Chem." volume="265" first="5648" last="5653">
            <title>Nucleotide sequence and expression of a plasmid-encoded chromate resistance determinant from Alcaligenes eutrophus.</title>
            <authorList>
                <person name="Nies A."/>
                <person name="Nies D.H."/>
                <person name="Silver S."/>
            </authorList>
            <dbReference type="PubMed" id="2180932"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC DNA]</scope>
        <scope>FUNCTION</scope>
    </reference>
    <reference key="2">
        <citation type="submission" date="2004-10" db="EMBL/GenBank/DDBJ databases">
            <title>Sequence and features of the Ralstonia metallidurans CH34 heavy metal plasmids pMOL28 and pMOL30.</title>
            <authorList>
                <person name="van der Lelie D."/>
                <person name="Monchy S."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="Benotmane M."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Mergeay M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference key="3">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <reference key="4">
        <citation type="journal article" date="2002" name="Arch. Microbiol." volume="179" first="15" last="25">
            <title>New genes involved in chromate resistance in Ralstonia metallidurans strain CH34.</title>
            <authorList>
                <person name="Juhnke S."/>
                <person name="Peitzsch N."/>
                <person name="Hubener N."/>
                <person name="Grosse C."/>
                <person name="Nies D.H."/>
            </authorList>
            <dbReference type="PubMed" id="12471500"/>
            <dbReference type="DOI" id="10.1007/s00203-002-0492-5"/>
        </citation>
        <scope>INDUCTION</scope>
    </reference>
    <comment type="function">
        <text evidence="3">This protein reduces chromate accumulation and is essential for chromate resistance.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cell inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="induction">
        <text evidence="2">By chromate; induction increases when cells are grown in the presence of high sulfate concentrations (3 mM SO4(2-)).</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the chromate ion transporter (CHR) (TC 2.A.51) family.</text>
    </comment>
    <comment type="sequence caution" evidence="5">
        <conflict type="erroneous initiation">
            <sequence resource="EMBL-CDS" id="CAI30234" version="1"/>
        </conflict>
        <text>Truncated N-terminus.</text>
    </comment>
    <dbReference type="EMBL" id="AJ313327">
        <property type="protein sequence ID" value="CAC42411.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="X90708">
        <property type="protein sequence ID" value="CAI30234.1"/>
        <property type="status" value="ALT_INIT"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP000355">
        <property type="protein sequence ID" value="ABF13061.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B35177">
        <property type="entry name" value="B35177"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011514877.1">
        <property type="nucleotide sequence ID" value="NC_007972.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_161712.1">
        <property type="nucleotide sequence ID" value="NC_006525.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="P17551"/>
    <dbReference type="TCDB" id="2.A.51.1.1">
        <property type="family name" value="the chromate ion transporter (chr) family"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF13061">
        <property type="protein sequence ID" value="ABF13061"/>
        <property type="gene ID" value="Rmet_6202"/>
    </dbReference>
    <dbReference type="GeneID" id="60825781"/>
    <dbReference type="KEGG" id="rme:Rmet_6202"/>
    <dbReference type="HOGENOM" id="CLU_018106_0_0_4"/>
    <dbReference type="OMA" id="CMFFGCL"/>
    <dbReference type="OrthoDB" id="1884249at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid pMOL28"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015109">
        <property type="term" value="F:chromate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046687">
        <property type="term" value="P:response to chromate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014047">
        <property type="entry name" value="Chr_Tranpt_l_chain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003370">
        <property type="entry name" value="Chromate_transpt"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02417">
        <property type="entry name" value="Chromate_transp"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004810">
        <property type="entry name" value="ChrA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00937">
        <property type="entry name" value="2A51"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at transcript level"/>
    <keyword id="KW-0997">Cell inner membrane</keyword>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0155">Chromate resistance</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-0614">Plasmid</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <keyword id="KW-0813">Transport</keyword>
    <feature type="chain" id="PRO_0000089654" description="Chromate transport protein">
        <location>
            <begin position="1"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="26"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="67"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="93"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="124"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="172"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="214"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="237"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="272"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="299"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="330"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="356"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="379"/>
            <end position="399"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000269" key="2">
        <source>
            <dbReference type="PubMed" id="12471500"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="3">
        <source>
            <dbReference type="PubMed" id="2180932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000303" key="4">
        <source>
            <dbReference type="PubMed" id="2180932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="5"/>
    <evidence type="ECO:0000312" key="6">
        <source>
            <dbReference type="EMBL" id="ABF13061.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000312" key="7">
        <source>
            <dbReference type="EMBL" id="CAC42411.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000312" key="8">
        <source>
            <dbReference type="EMBL" id="CAI30234.1"/>
        </source>
    </evidence>
    <sequence length="401" mass="42642" checksum="6DC963FBADA6DBC5" modified="1990-08-01" version="1">MNSPQPPDTTAAGSVHTAPTYTLRQLVMYFLRLGTLGFGGPVALAGYMHRDLVEAKQWITDADYKEGLALAQLAPGPLAAQLAIYLGYVHYRIVGATLVGVAFVLPSFLMVLALGWAYVRFGGLTWMQSVFYGVGAAVIGIIAISAYKLTKKSVGNDKLLWFIYLVLVAVTVITESEVAWLFLAAGVLVWFWRAPPKWLRQGKMNAFAATPLPAASGMMSTLDWPLLSQIGVFFAKAGAFVFGSGLAIVPFLYGGVVTEYHWLNDKQFVDAVAVAMITPGPVVITVGFIGYLVAGLPGACVAAAATFLPCYLFTVLPAPYFKKYGKLPAILAFVDGVTAAAIGAITGAVIVLAKRSIVDIPTALLALVTVALLLKFKKLSEPMIVAGAALIGLVAYPLLHH</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="60" xmlns="http://uniprot.org/uniprot">
    <accession>Q1L9K9</accession>
    <name>Q1L9K9_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Conjugative transfer protein, possible DNA relaxase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">traI</name>
        <name evidence="3" type="ordered locus">Rmet_6308</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3 4">
        <name>pMOL28</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid pMOL28</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000355">
        <property type="protein sequence ID" value="ABF13167.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008649463.1">
        <property type="nucleotide sequence ID" value="NC_007972.2"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF13167">
        <property type="protein sequence ID" value="ABF13167"/>
        <property type="gene ID" value="Rmet_6308"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6308"/>
    <dbReference type="HOGENOM" id="CLU_620647_0_0_4"/>
    <dbReference type="OMA" id="RMRTGML"/>
    <dbReference type="OrthoDB" id="819661at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid pMOL28"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011119">
        <property type="entry name" value="Unchr_helicase_relaxase_TraI"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07514">
        <property type="entry name" value="TraI_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TraI_2" evidence="2">
        <location>
            <begin position="10"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="249"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="270"/>
            <end position="304"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF13167.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="413" mass="44823" checksum="3B1CE6F0C08055D8" modified="2006-05-30" version="1">MSTHTHSSMPAAALLSAHESRIALIRQYANEVSGDLFAVKWLSILTRCADWFSSMPLRFAEHAEPGGAFRATVEAAYFAMRLSGAQKFGADQPSERRRMLEPQYLYALFLAACCSRLDEPCRHFQFYRDSDGAEWIPAAHGAFGPWVGTSNYRVTRREAIQPIERMRTALLAREILGSERLSAFDSQVLTELFGAINPDPRPTGLETLLHKVVRQSIDTLTQFEIKARKAEFPPDSTPVPTAERLEKAVNPSLAQADAPVPAPPPATSTQHADGKGNVSPAESAGLGSQAQVESPDATASDASAARNVVQLDGARSLRKELSADDPFKALAGSSNLMREFFKALAQDVASGKAKVARIDGKVSISKRSLGNFGLASDTLVEHLRKGKHLYKVDGQNILLVDEVGRLIAPETAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LAM8</accession>
    <name>Q1LAM8_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">HPt domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_5939</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12798.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518144.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5939"/>
    <dbReference type="EnsemblBacteria" id="ABF12798">
        <property type="protein sequence ID" value="ABF12798"/>
        <property type="gene ID" value="Rmet_5939"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5939"/>
    <dbReference type="eggNOG" id="COG2198">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1853569_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.160">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036641">
        <property type="entry name" value="HPT_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008207">
        <property type="entry name" value="Sig_transdc_His_kin_Hpt_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01627">
        <property type="entry name" value="Hpt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47226">
        <property type="entry name" value="SSF47226"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50894">
        <property type="entry name" value="HPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HPt" evidence="2">
        <location>
            <begin position="29"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="modified residue" description="Phosphohistidine" evidence="1">
        <location>
            <position position="68"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF12798.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="138" mass="14855" checksum="89B874E21D7A4C86" modified="2006-05-30" version="1">MTSSGTPSVLTTEECQAFGPALASAAREDPTVYQLLLTRLIDANHGDLTDLRRAAKLRDWAGVHRSIHRLKGSAALARCPTLVAAGKSIESAAKYRKAAVVNALLPRYVAIVTEFNDRLRDLRSNRRSAPGGGFVQGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LAW2</accession>
    <name>Q1LAW2_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Glycose-acyl transferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">wcaF</name>
        <name evidence="2" type="ordered locus">Rmet_5855</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12714.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518062.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5855"/>
    <dbReference type="EnsemblBacteria" id="ABF12714">
        <property type="protein sequence ID" value="ABF12714"/>
        <property type="gene ID" value="Rmet_5855"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5855"/>
    <dbReference type="eggNOG" id="COG0110">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_051638_7_2_4"/>
    <dbReference type="OMA" id="IWAPWNL"/>
    <dbReference type="OrthoDB" id="1878940at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001451">
        <property type="entry name" value="Hexapep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011004">
        <property type="entry name" value="Trimer_LpxA-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00132">
        <property type="entry name" value="Hexapep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51161">
        <property type="entry name" value="SSF51161"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="178"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="180"/>
            <end position="206"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF12714.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="206" mass="22782" checksum="B78E3418C06ED4B6" modified="2006-05-30" version="1">MFQDLSTFRMPPGFRGRSAVVVQLWWCVQSLFFHHSPHFANGFRCWLLRRFGARVGHHVVIRPSVRITYPWKVSIGDYAWIGDGAELYSLAEIEIGNHAVVSQFSYLCGGDHDYRSSDFAIRGRPIRIADEAWVASHTFIAPGVSIGKGAVIGARSAVFTDMPAGMLCVGSPCRPLRPRLDTASQNQTRPHSQAAAPSELDSAQVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="109" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LB09</accession>
    <name>Q1LB09_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Peptide chain release factor 3</fullName>
            <shortName evidence="5">RF-3</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5 7" type="primary">prfC</name>
        <name evidence="7" type="ordered locus">Rmet_5808</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="8">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="8">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Increases the formation of ribosomal termination complexes and stimulates activities of RF-1 and RF-2. It binds guanine nucleotides and has strong preference for UGA stop codons. It may interact directly with the ribosome. The stimulation of RF-1 and RF-2 is significantly reduced by GTP and GDP, but not by GMP.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 5">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. PrfC subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12667.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011520203.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5808"/>
    <dbReference type="EnsemblBacteria" id="ABF12667">
        <property type="protein sequence ID" value="ABF12667"/>
        <property type="gene ID" value="Rmet_5808"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5808"/>
    <dbReference type="eggNOG" id="COG4108">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002794_2_1_4"/>
    <dbReference type="OMA" id="GFVFKIH"/>
    <dbReference type="OrthoDB" id="164090at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016149">
        <property type="term" value="F:translation release factor activity, codon specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006449">
        <property type="term" value="P:regulation of translational termination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd04169">
        <property type="entry name" value="RF3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.3280">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00072">
        <property type="entry name" value="Rel_fac_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035647">
        <property type="entry name" value="EFG_III/V"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004161">
        <property type="entry name" value="EFTu-like_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031157">
        <property type="entry name" value="G_TR_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004548">
        <property type="entry name" value="PrfC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032090">
        <property type="entry name" value="RF3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038467">
        <property type="entry name" value="RF3_dom_3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041732">
        <property type="entry name" value="RF3_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000795">
        <property type="entry name" value="T_Tr_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43556">
        <property type="entry name" value="PTHR43556"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00009">
        <property type="entry name" value="GTP_EFTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03144">
        <property type="entry name" value="GTP_EFTU_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16658">
        <property type="entry name" value="RF3_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00315">
        <property type="entry name" value="ELONGATNFCT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="SSF50447"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54980">
        <property type="entry name" value="SSF54980"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00503">
        <property type="entry name" value="prfC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00301">
        <property type="entry name" value="G_TR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51722">
        <property type="entry name" value="G_TR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 5" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0614">Plasmid</keyword>
    <keyword evidence="5" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tr-type G" evidence="6">
        <location>
            <begin position="9"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="18"/>
            <end position="25"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="92"/>
            <end position="96"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="146"/>
            <end position="149"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009978"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABF12667.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="530" mass="59000" checksum="7CD8D6CC9F7C13FF" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="63" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LB28</accession>
    <name>Q1LB28_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_5789</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12648.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008648807.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5789"/>
    <dbReference type="EnsemblBacteria" id="ABF12648">
        <property type="protein sequence ID" value="ABF12648"/>
        <property type="gene ID" value="Rmet_5789"/>
    </dbReference>
    <dbReference type="GeneID" id="60823535"/>
    <dbReference type="KEGG" id="rme:Rmet_5789"/>
    <dbReference type="HOGENOM" id="CLU_188872_0_0_4"/>
    <dbReference type="OMA" id="RKFIDCR"/>
    <dbReference type="OrthoDB" id="2067433at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009409">
        <property type="entry name" value="DUF1059"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06348">
        <property type="entry name" value="DUF1059"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF12648.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="64" mass="7183" checksum="C25520EF1F25ED7F" modified="2006-05-30" version="1">MGRKFIDCRDYPSQMHCTVALSADTEEELLEAAVQHAVQVHEHQDTPELRSQLKACFKDGSPPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LB39</accession>
    <name>Q1LB39_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Porin, Gram-negative type</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">Rmet_5778</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="10">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="10">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12637.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011520176.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5778"/>
    <dbReference type="EnsemblBacteria" id="ABF12637">
        <property type="protein sequence ID" value="ABF12637"/>
        <property type="gene ID" value="Rmet_5778"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5778"/>
    <dbReference type="eggNOG" id="COG3203">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038238_2_0_4"/>
    <dbReference type="OMA" id="FQDYRIN"/>
    <dbReference type="OrthoDB" id="1064531at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009279">
        <property type="term" value="C:cell outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046930">
        <property type="term" value="C:pore complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015288">
        <property type="term" value="F:porin activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034220">
        <property type="term" value="P:ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00342">
        <property type="entry name" value="gram_neg_porins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.160.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033900">
        <property type="entry name" value="Gram_neg_porin_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023614">
        <property type="entry name" value="Porin_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001702">
        <property type="entry name" value="Porin_Gram-ve"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002299">
        <property type="entry name" value="Porin_Neis"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13609">
        <property type="entry name" value="Porin_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00182">
        <property type="entry name" value="ECOLNEIPORIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00184">
        <property type="entry name" value="NEISSPPORIN"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0406">Ion transport</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0614">Plasmid</keyword>
    <keyword evidence="5" id="KW-0626">Porin</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1134">Transmembrane beta strand</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193028" evidence="7">
        <location>
            <begin position="22"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="domain" description="Porin_4" evidence="8">
        <location>
            <begin position="11"/>
            <end position="319"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF13609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ABF12637.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="358" mass="38319" checksum="D7AEB1152EEF49BA" modified="2006-05-30" version="1">MKKSASLWLLAATVVPGTTYAESGVNLYGIVSSAVRYTSNLDGRHHDQVALVSGGMVGSRFGLRGSEDLGGGNRAIFQLEAGIGSDDGRAAYNAFFGRQAWAGLSGNWGSLTFGRQYNALNNIGWAFNPLDQGWGNFWSDPLYVGGDIFFQDYRINNSVVYQNTVGPFSVQLDYGAGEQPGNQSRGTTLGGGVKYQQGPLALGVAYDQRSSTDGTNTIRNYSVGGSYAFGKATAYLGHMGRRETASDASFATSFIGLGYQLTPALHLSGAYYRYQQRGAVTTQFQETPILLGSGNADSVALVGDYALSKRTSLYVEADVVYARGGTVGRETEYWAGTPVTDIDSTTRVGVMVGMRHQF</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="67" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LBF0</accession>
    <name>Q1LBF0_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">Rmet_5667</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="10">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="10">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12526.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008647667.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5667"/>
    <dbReference type="DNASU" id="4042531"/>
    <dbReference type="EnsemblBacteria" id="ABF12526">
        <property type="protein sequence ID" value="ABF12526"/>
        <property type="gene ID" value="Rmet_5667"/>
    </dbReference>
    <dbReference type="GeneID" id="60824484"/>
    <dbReference type="KEGG" id="rme:Rmet_5667"/>
    <dbReference type="eggNOG" id="ENOG5032Z2S">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_126480_0_0_4"/>
    <dbReference type="OMA" id="FRDHKTS"/>
    <dbReference type="OrthoDB" id="1764449at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027379">
        <property type="entry name" value="CLS_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018649">
        <property type="entry name" value="SHOCT"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13396">
        <property type="entry name" value="PLDc_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09851">
        <property type="entry name" value="SHOCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0614">Plasmid</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="44"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="domain" description="PLDc_N" evidence="8">
        <location>
            <begin position="23"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="domain" description="SHOCT" evidence="7">
        <location>
            <begin position="98"/>
            <end position="124"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF09851"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF13396"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ABF12526.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="126" mass="14635" checksum="82C6898757F266FE" modified="2006-05-30" version="1">MVIQEGFSFGNFIIDVFSIFLFILWFWLLITISGDLFRRHDVSGFAKVLWVIFLIVLPYIGVFAYILTQGRGMAERNQERAREARNELRQVVGFSVADELEKLDRLKASGSISEDEFKRLRAKLVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LBH3</accession>
    <name>Q1LBH3_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Dioxygenase</fullName>
            <ecNumber evidence="4">1.13.11.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">carX</name>
        <name evidence="5" type="ordered locus">Rmet_5644</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="6">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="6">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="4">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
        <text evidence="4">Binds 1 Fe(2+) ion per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the carotenoid oxygenase family.</text>
    </comment>
    <dbReference type="EC" id="1.13.11.-" evidence="4"/>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12503.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5644"/>
    <dbReference type="EnsemblBacteria" id="ABF12503">
        <property type="protein sequence ID" value="ABF12503"/>
        <property type="gene ID" value="Rmet_5644"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5644"/>
    <dbReference type="eggNOG" id="COG3670">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016472_0_2_4"/>
    <dbReference type="OMA" id="SYRNRWV"/>
    <dbReference type="OrthoDB" id="389495at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016702">
        <property type="term" value="F:oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004294">
        <property type="entry name" value="Carotenoid_Oase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10543">
        <property type="entry name" value="PTHR10543"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03055">
        <property type="entry name" value="RPE65"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="3 4" id="KW-0408">Iron</keyword>
    <keyword evidence="2 4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-0614">Plasmid</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU364048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABF12503.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="478" mass="52126" checksum="B410014578822482" modified="2006-05-30" version="1">MEDSLSEVEGAAGDVSDVSEVDNFAPVTQEVDASDLCVRGVLPAGLQGTLYRIGPNPRFPQPEAHWFGGDGMVHVFAIDDGRVSYRNRWVRTPKWRDEDRAGRPLFRAFAGKLPDAPEWSGSDGGVANTNIIWHGGALLALEEAHLPTAVTPRAIDTLGYMEYGPLKGPFTAHPKTDPRTGELLFFGYNADGKLTPGVQVGAIDAQGRLTHYARLQAPYPSMIHDFMATPGHLLIPVLPLTGSLARVERGGPGYAWEPEKPAMIGVLPRGGGADDVRWFEGPACYVFHVMNAWEEGEGGQRILADVMVYDEPPLFPHADGSPGDPARQQAYLTRWTFDLASDIRRFTAERLDDTAGEFPRIDDRVSGFRYRHGWFAASHPEYGEGALNGIMHLDMQDGTRDAYWLPRGDITSEPVFAPGGGAHGGEGDGWLLAVVWRAAAQCSELLVFDARHVARGPLATVELPQRVPFGFHGNWVAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-12-02" version="70" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LBI9</accession>
    <name>Q1LBI9_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Small permease component of tripartite tricarboxylate transporter (TctB)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">tctB</name>
        <name evidence="2" type="ordered locus">Rmet_5628</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12487.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5628"/>
    <dbReference type="EnsemblBacteria" id="ABF12487">
        <property type="protein sequence ID" value="ABF12487"/>
        <property type="gene ID" value="Rmet_5628"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5628"/>
    <dbReference type="eggNOG" id="ENOG5032FF5">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_110735_4_0_4"/>
    <dbReference type="OMA" id="ERWFLVP"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009936">
        <property type="entry name" value="DUF1468"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07331">
        <property type="entry name" value="TctB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="32"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="64"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="104"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="141"/>
            <end position="160"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF12487.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="179" mass="19552" checksum="FCF19616D57EA17D" modified="2006-05-30" version="1">MGWGATIRGDTFMSEQLHDTAPAAITVRAAEIATALLIAIGAVVVMVSNYQVGAGWEPDGPQAGYFPMRIGAIIFIACMVVLWQAWHGEKNKVFVTWPQLRQVAVILVPLTVYVALIAFLGIYVASAIFIAGFMLVIGKFAWWRAVLTGVAINLVLFWIFEMQFRVPLPKGPLEAALGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="63" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LBJ8</accession>
    <name>Q1LBJ8_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_5619</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12478.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011520024.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5619"/>
    <dbReference type="EnsemblBacteria" id="ABF12478">
        <property type="protein sequence ID" value="ABF12478"/>
        <property type="gene ID" value="Rmet_5619"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5619"/>
    <dbReference type="eggNOG" id="COG2931">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_688618_0_0_4"/>
    <dbReference type="OMA" id="KYTLAAY"/>
    <dbReference type="OrthoDB" id="2029905at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008160">
        <property type="entry name" value="Collagen"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01391">
        <property type="entry name" value="Collagen"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193039" evidence="2">
        <location>
            <begin position="25"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="182"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="215"/>
            <end position="252"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF12478.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="400" mass="37976" checksum="DEAFD3EE207BD969" modified="2006-05-30" version="1">MKNTQRKLAVLPLACLAATLAACGGDSSESGTAAATPTSTPAATPAGSMIYTGVTDPTSSIGVNGDFYLNSATSTLFGPKANSAWSAGVSLIGPAGTLIRYGTRDPIASDGNDGDFYLNTATSTLFGPKAAGAWPMPGKSLIGATGAVGATGATGATGATGATGLQGIQGVTGPTGVTGATGATGAGVTGPQGIQGIQGPTGPAGAAGPTGATGATGATGVTGPSGVTGPTGPQGVQGIQGPTGTTGATGATGATGATGATGATGATGPALYTANFVVGNGTTFGNATYIMTGSGLTPIATQPYLGVLLPINCSTLTLSTAVAGIPPAPYAMEVFKIQPPSTTVTSTGLRCQANSSGASCTQTQTGTYAASAGEVLQVQLTGTTPFNTWSGAVYATLTCQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LCA0</accession>
    <name>Q1LCA0_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative Acyl-CoA synthetase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_5367</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12226.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519773.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5367"/>
    <dbReference type="EnsemblBacteria" id="ABF12226">
        <property type="protein sequence ID" value="ABF12226"/>
        <property type="gene ID" value="Rmet_5367"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5367"/>
    <dbReference type="eggNOG" id="COG1042">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007415_3_1_4"/>
    <dbReference type="OMA" id="WCDAVLC"/>
    <dbReference type="OrthoDB" id="445933at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.261">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013815">
        <property type="entry name" value="ATP_grasp_subdomain_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003781">
        <property type="entry name" value="CoA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032875">
        <property type="entry name" value="Succ_CoA_lig_flav_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016102">
        <property type="entry name" value="Succinyl-CoA_synth-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13380">
        <property type="entry name" value="CoA_binding_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13607">
        <property type="entry name" value="Succ_CoA_lig"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00881">
        <property type="entry name" value="CoA_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52210">
        <property type="entry name" value="SSF52210"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CoA_binding" evidence="1">
        <location>
            <begin position="24"/>
            <end position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF12226.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="715" mass="74754" checksum="3D98E77D4C0AA71F" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-12-02" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LCD3</accession>
    <name>Q1LCD3_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Protein MgtC</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Rmet_5334</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="9">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="9">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cell inner membrane</location>
            <topology evidence="7">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the MgtC/SapB family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12193.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5334"/>
    <dbReference type="EnsemblBacteria" id="ABF12193">
        <property type="protein sequence ID" value="ABF12193"/>
        <property type="gene ID" value="Rmet_5334"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5334"/>
    <dbReference type="eggNOG" id="COG1285">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_079292_0_0_4"/>
    <dbReference type="OMA" id="VIMREEG"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003416">
        <property type="entry name" value="MgtC/SapB/SrpB/YhiD_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33778">
        <property type="entry name" value="PTHR33778"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02308">
        <property type="entry name" value="MgtC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01837">
        <property type="entry name" value="MGTCSAPBPROT"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0614">Plasmid</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="14"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="44"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="73"/>
            <end position="90"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009298"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU365041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ABF12193.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="240" mass="25370" checksum="616AE02ADAE61DB3" modified="2006-05-30" version="1">MSIEAIQNINLESLLDTSISLVTAFVLGGVIGYERQYRQRTAGLRTNVLVAVGAAIFVDMANRLAGHDGAVRVVAYVVSGIGFLGAGVIMREEGNVRGLNTAATLWGSAAVGASAGADLIIEAGLGSMFVLAANTLLRPVVNRINQQPIDTPAVEVTNTVYVITPRHRQKEALRLLSDMLEASNHPTRDLVVHAFGANDIEIEAVLMATSVDGDELDAVVARLSACEAVTQAFWSPSTTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="62" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LCP3</accession>
    <name>Q1LCP3_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_5224</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12083.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519629.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5224"/>
    <dbReference type="EnsemblBacteria" id="ABF12083">
        <property type="protein sequence ID" value="ABF12083"/>
        <property type="gene ID" value="Rmet_5224"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5224"/>
    <dbReference type="eggNOG" id="COG4313">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_066206_0_0_4"/>
    <dbReference type="OrthoDB" id="1322015at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025737">
        <property type="entry name" value="Phenol_MetA_deg_put"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13557">
        <property type="entry name" value="Phenol_MetA_deg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193315" evidence="1">
        <location>
            <begin position="26"/>
            <end position="322"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF12083.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="322" mass="34135" checksum="68B9242B8FF9BAEC" modified="2006-05-30" version="1">MKRASGPVLAAIAAGSLTAPLAGHATEGALGRPVAGTSVSSNVAVIPDQPVWIANLQQLYMDGSISGSRQVPIGGRTTLGVDAKIAFTLATLMKVWDTGPGAWHFASSFTLPYVWTSVHANAGVTAGGASFSRGTSDRASNLYDIYFSPIIAGFHFSQNSHMALSFNVWAPTGQYDKNALANPSLNNWTFVPQAAFTHVMPQYGLEFNGVAAFQFYTRNSATDYQNAPLFTLDVMGLKKFGAWGTGIVMGTVQQLGNDTGPTADRLNGFRGHDFTIGPIVTYDTKLGGKAPLSFSVRWVPTVTSKNRLDSTATVLATATLVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-10-07" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LD73</accession>
    <name>Q1LD73_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative signaling protein (GGDEF domain)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_5041</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11903.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5041"/>
    <dbReference type="EnsemblBacteria" id="ABF11903">
        <property type="protein sequence ID" value="ABF11903"/>
        <property type="gene ID" value="Rmet_5041"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5041"/>
    <dbReference type="eggNOG" id="COG3706">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_731171_0_0_4"/>
    <dbReference type="OMA" id="CAGLFID"/>
    <dbReference type="OrthoDB" id="1635706at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01949">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000160">
        <property type="entry name" value="GGDEF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029787">
        <property type="entry name" value="Nucleotide_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00990">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00267">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55073">
        <property type="entry name" value="SSF55073"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00254">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50887">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="38"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="68"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="101"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="125"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="150"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="179"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="domain" description="GGDEF" evidence="2">
        <location>
            <begin position="246"/>
            <end position="385"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF11903.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="400" mass="44452" checksum="1FB1C081D100A4FF" modified="2006-05-30" version="1">MSEADVEMSFVVDPVRVEFSDPGLEREFQQHHLRTTRASLRVTLVFCSLFYLAFSLTDLAALGYGTSFLVLLLGRITVALTACGGLYLIRRKTESVLAHRAVASAAEVVGMAIFMLVVWFRPGELPWHAMSLCIMLIVIYVFIPNRLITAIGIALGATAAFIALAVIRGESRFSDEVTMAMLLLLTNSFGIIAARRYHRLWRDEYRALSTLKALSIRDHLTACFNRRHLHTTLLPQVLERARAERRWLTLMVCDIDHFKRVNDNHGHQGGDAVLMHVAMLLQDRIHEPRDSVVRYGGEEFLLVMPQMDFESAASLAEDMRAAVAGSEVPGPDGKSMRATVSIGVLSVDFSASDTEVTESALIAAADMLLYEAKRAGRNSIRTRLWADMVSSHAVLSMRTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LDC9</accession>
    <name>Q1LDC9_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Sodium/hydrogen exchanger</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Rmet_4985</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="9">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="9">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11847.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519395.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4985"/>
    <dbReference type="DNASU" id="4041847"/>
    <dbReference type="EnsemblBacteria" id="ABF11847">
        <property type="protein sequence ID" value="ABF11847"/>
        <property type="gene ID" value="Rmet_4985"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4985"/>
    <dbReference type="eggNOG" id="COG0025">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005912_8_1_4"/>
    <dbReference type="OMA" id="AGMMMNY"/>
    <dbReference type="OrthoDB" id="1194802at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015299">
        <property type="term" value="F:solute:proton antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006814">
        <property type="term" value="P:sodium ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006153">
        <property type="entry name" value="Cation/H_exchanger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018422">
        <property type="entry name" value="Cation/H_exchanger_CPA1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10110">
        <property type="entry name" value="PTHR10110"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00999">
        <property type="entry name" value="Na_H_Exchanger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0406">Ion transport</keyword>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0614">Plasmid</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0915">Sodium</keyword>
    <keyword evidence="5" id="KW-0739">Sodium transport</keyword>
    <keyword evidence="1 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="7"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="34"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="74"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="103"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="129"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="173"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="203"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="256"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="288"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="320"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="392"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="domain" description="Na_H_Exchanger" evidence="7">
        <location>
            <begin position="14"/>
            <end position="418"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ABF11847.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="435" mass="45624" checksum="0B7FD8F2137A4381" modified="2006-05-30" version="1">MDVAIELTTRVGVLLFFIAICHVIAQRVRLPDPIILTAAGVTIGFGYVALEHVAPAFAANTIAPFLSPAMPPEAYLWIFLPPILFQAAIEVDSSGFVADLGPILLLAIGAVVAATLSVGAVAYLTSGQGLIACLLLGAIVSTTDPSTVIGLFQSSGAPARLIRLVEGESLFNDAAAIAITTLLTGVISVMPTHDAAHFPLAQFLLSLLGGCAVGWLVGVVLVFVSGAMRSPPFAEYSLSLALPNLIYPFAERVLHVSGVAAVVCAGLVVGRIMLSRRPASHVKLFRQLWLYQATIASASVLLLATAHVPSLLDDLRMIDVVILLLVLVAAVASRFGILSVSMPMMSRIGICKPLPVSHQLLIAWGGVRGPVTLTLAICLARNQDLAPDTRHFAAAMAAGFVLLNLFFNGLTLKLFARWLGVAAPGGRRPAAIEVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="56" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LDZ7</accession>
    <name>Q1LDZ7_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Putative lipoprotein entericidin AB</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">ecnAB</name>
        <name evidence="6" type="ordered locus">Rmet_4767</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="7">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="7">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the EcnA/EcnB lipoprotein family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11629.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008651235.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF11629">
        <property type="protein sequence ID" value="ABF11629"/>
        <property type="gene ID" value="Rmet_4767"/>
    </dbReference>
    <dbReference type="GeneID" id="60821644"/>
    <dbReference type="KEGG" id="rme:Rmet_4767"/>
    <dbReference type="eggNOG" id="COG5510">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_193827_3_0_4"/>
    <dbReference type="OrthoDB" id="2055071at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009636">
        <property type="term" value="P:response to toxic substance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012556">
        <property type="entry name" value="Entericidin"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08085">
        <property type="entry name" value="Entericidin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0614">Plasmid</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193406" evidence="5">
        <location>
            <begin position="23"/>
            <end position="42"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABF11629.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="42" mass="4548" checksum="C149744FFAB45EF7" modified="2006-05-30" version="1">MKKGWIWCVLVATLLAGCNTMAGLGQDIQKGGQKLENSAERK</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="99" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LDZ9</accession>
    <name>Q1LDZ9_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Putative inner membrane protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ordered locus">Rmet_4765</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="12">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="12">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11627.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519198.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4765"/>
    <dbReference type="EnsemblBacteria" id="ABF11627">
        <property type="protein sequence ID" value="ABF11627"/>
        <property type="gene ID" value="Rmet_4765"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4765"/>
    <dbReference type="eggNOG" id="COG1253">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015237_4_0_4"/>
    <dbReference type="OMA" id="EHTMVRN"/>
    <dbReference type="OrthoDB" id="1155802at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04590">
        <property type="entry name" value="CBS_pair_CorC_HlyC_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.580.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.465.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002550">
        <property type="entry name" value="CNNM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036318">
        <property type="entry name" value="FAD-bd_PCMH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016169">
        <property type="entry name" value="FAD-bd_PCMH_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044751">
        <property type="entry name" value="Ion_transp-like_CBS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005170">
        <property type="entry name" value="Transptr-assoc_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00571">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03471">
        <property type="entry name" value="CorC_HlyC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01595">
        <property type="entry name" value="DUF21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00116">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01091">
        <property type="entry name" value="CorC_HlyC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="SSF54631"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56176">
        <property type="entry name" value="SSF56176"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51371">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51846">
        <property type="entry name" value="CNNM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 7" id="KW-0129">CBS domain</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-0614">Plasmid</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="CNNM transmembrane" evidence="10">
        <location>
            <begin position="1"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="9">
        <location>
            <begin position="215"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="9">
        <location>
            <begin position="279"/>
            <end position="341"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ABF11627.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="437" mass="47468" checksum="325BD7396A08EABD" modified="2006-05-30" version="1">MEIAILLALILLNGLFAMSEIALVTARKARLQRHIDEGDRGAMAAVKLGEDPTRFLSTVQIGITSIGVLNGVVGESTLAAPLGVWLQGFGISATTAGYVATAIVVAGLTYFSIVLGELVPKRLGQLAPEAIARMAARPISFLAVASTPFVKLLSSSTRLVLRLLGVKTNRGPAVTEEEIHALLVEGSEAGIIEQQEHTMVRNVFRLDDRQLASLMVPRGDVVYLDVEESEDENLRRIEESDHARFPVVRGGMHDILGVVSARQMLAKRLRGEKAELTAVLQPAVFVPESVTGMELLENFRSSGGQVAFVIDEYGEVLGLVTLQDLVEAITGEFKPDAAGDEWAVQRDDGSWLLDGLIPIPELKDRIGLRAVPEEAKERYHTLSGMLLLLLGRLPQTTDAVQWEDWKFEIVDMDGKRIDKVLASRVPAEDGPEPDTTG</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="64" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LE21</accession>
    <name>Q1LE21_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_4743</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11605.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519177.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4743"/>
    <dbReference type="EnsemblBacteria" id="ABF11605">
        <property type="protein sequence ID" value="ABF11605"/>
        <property type="gene ID" value="Rmet_4743"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4743"/>
    <dbReference type="eggNOG" id="COG4539">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_081702_2_0_4"/>
    <dbReference type="OMA" id="IQFIGHY"/>
    <dbReference type="OrthoDB" id="1934114at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009305">
        <property type="entry name" value="Mpo1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28026">
        <property type="entry name" value="PTHR28026"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06127">
        <property type="entry name" value="DUF962"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="21"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="52"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="75"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="100"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="133"/>
            <end position="153"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF11605.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="165" mass="17933" checksum="AE0B7C254C6272C1" modified="2006-05-30" version="1">MKTLIDHLSNYAAYHRDTRNVVTHFIGIPMIVLAVTALLGRPALPLGDGARYLTPAMIVYALSCLFYLRLSFAFGVVMAVILAFFVWAGAWLAALPTAAWLASGIGLFVVGWVIQFVGHYFEGRKPAFVDDLVGLLVGPLFLVAETAFALGLARETRDAMLAHAH</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LE71</accession>
    <name>Q1LE71_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative lytic transglycosylase, catalytic</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_4693</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the transglycosylase Slt family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11555.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4693"/>
    <dbReference type="CAZy" id="GH23">
        <property type="family name" value="Glycoside Hydrolase Family 23"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF11555">
        <property type="protein sequence ID" value="ABF11555"/>
        <property type="gene ID" value="Rmet_4693"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4693"/>
    <dbReference type="eggNOG" id="COG0741">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_065765_1_0_4"/>
    <dbReference type="OMA" id="RYRLEWH"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008933">
        <property type="term" value="F:lytic transglycosylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000270">
        <property type="term" value="P:peptidoglycan metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023346">
        <property type="entry name" value="Lysozyme-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000189">
        <property type="entry name" value="Transglyc_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008258">
        <property type="entry name" value="Transglycosylase_SLT_dom_1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01464">
        <property type="entry name" value="SLT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53955">
        <property type="entry name" value="SSF53955"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00922">
        <property type="entry name" value="TRANSGLYCOSYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SLT" evidence="2">
        <location>
            <begin position="92"/>
            <end position="190"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF11555.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="215" mass="23485" checksum="E80E4163CA9C2CB2" modified="2006-05-30" version="1">MYWEGTPMTERKPSTLPRTILFTVVVIVGLAFARVARAQIFGAVSADGSITLTNTAGNPHLHLLVAGNPPAERKPREPRDLPTDAALFATFISEASRNSHLPPELIRAVIAIESRFNPNAVSRKGAIGLMQLMPDTAKRFSTGDMLNPRDNILAGAQYLRYLLDLFRDDLELALAAYNAGENAVIQAGNRIPAYAETQAYVPMVMAHYRRLLASG</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="62" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LEJ3</accession>
    <name>Q1LEJ3_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_4571</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11433.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519021.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4571"/>
    <dbReference type="EnsemblBacteria" id="ABF11433">
        <property type="protein sequence ID" value="ABF11433"/>
        <property type="gene ID" value="Rmet_4571"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4571"/>
    <dbReference type="eggNOG" id="COG0807">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026445_1_0_4"/>
    <dbReference type="OMA" id="AGTKWQY"/>
    <dbReference type="OrthoDB" id="935342at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012469">
        <property type="entry name" value="DUF1688"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31687">
        <property type="entry name" value="PTHR31687"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07958">
        <property type="entry name" value="DUF1688"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="415"/>
            <end position="435"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF11433.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="435" mass="47208" checksum="A21EE0FC011013F6" modified="2006-05-30" version="1">MNEFPEGGRAGRDDGMGSGWHSPVPPDHPAAALLSAEAVRTRCAVVTDFVASGESELFTWHPDRVHAIADYVAATIRRRYPTLQVPYHSRWRHFESGGPGQATINRWQILCERAGMSGPEHREERARIGIDLVIPSVLLDAGAGPDWRYRDAASDMMLTRSEGLGVASFDLFARGGFSAGQGDPLRSDADRLCRIDASTIASAFQVAQHNPLVGLEGRAGLLRRLGEVMQDTPAVFGSPARLGNLYDYLASHAREGRIEASFVLRTLLVALGPVWPGRLQIQGISLGDCWRHPAAPEGMVPFHKLTQWLTYSLLEPLEDAGLTVTGLDALTGLPEYRNGGLLYDFELMVPRDATFASRAHAVDEPEIVEWRALTVTGLDLIADGVRQALGLSEADFPLARVLEGGTWAAGRRVAAQRRPPGGPPPFAIQSDGTVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="92" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LF77</accession>
    <name>Q1LF77_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">4Fe-4S ferredoxin, iron-sulfur binding</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">asfB</name>
        <name evidence="5" type="ordered locus">Rmet_4334</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="6">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="6">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11199.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008652374.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4334"/>
    <dbReference type="EnsemblBacteria" id="ABF11199">
        <property type="protein sequence ID" value="ABF11199"/>
        <property type="gene ID" value="Rmet_4334"/>
    </dbReference>
    <dbReference type="GeneID" id="60823162"/>
    <dbReference type="KEGG" id="rme:Rmet_4334"/>
    <dbReference type="eggNOG" id="COG1146">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_152535_0_0_4"/>
    <dbReference type="OMA" id="YCPTDAL"/>
    <dbReference type="OrthoDB" id="1873445at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00037">
        <property type="entry name" value="Fer4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0408">Iron</keyword>
    <keyword evidence="3" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0614">Plasmid</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="4">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="4">
        <location>
            <begin position="31"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABF11199.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="111" mass="12225" checksum="881EC4B59489242D" modified="2006-05-30" version="1">MIELISESRCTGCNICVRVCPVNVFDAVPQAAPTIARQADCQTCFMCEAWCPEDAMFVSPNAEASTGVSEQSLVREELLGSYRKNIGWNRGFPEKRGVDDSYQLLSMPGAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-10-07" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LFH1</accession>
    <name>Q1LFH1_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_4238</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11105.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF11105">
        <property type="protein sequence ID" value="ABF11105"/>
        <property type="gene ID" value="Rmet_4238"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4238"/>
    <dbReference type="HOGENOM" id="CLU_1659314_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF11105.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="159" mass="17374" checksum="9ACE84A05DE20A1F" modified="2006-05-30" version="1">MVPFLPQQVLQRAILPADCYVTMADRVLAQVAAASAAVACEGPCSDTNRDLHFEPTRGALSDAHILVLWDTCGDRRLPIRRTLPLRPTSIAFPDYSMNLVFPSVQLLSLCPRRCAPKGVILAHSAYPGQLVSTEHRAMPRSIKVMFDLALERGSASSMS</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="59" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LFM6</accession>
    <name>Q1LFM6_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_4183</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11050.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518671.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4183"/>
    <dbReference type="EnsemblBacteria" id="ABF11050">
        <property type="protein sequence ID" value="ABF11050"/>
        <property type="gene ID" value="Rmet_4183"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4183"/>
    <dbReference type="eggNOG" id="ENOG5031D9T">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_182104_0_0_4"/>
    <dbReference type="OMA" id="PWVTSHA"/>
    <dbReference type="OrthoDB" id="1870918at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="14"/>
            <end position="41"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF11050.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="98" mass="10706" checksum="ED9F2231F69994E3" modified="2006-05-30" version="1">MRSLANLFSPPTPEKIAEKELQDMRLTLFQAERRLLEAQMQVDYYRNMIAFLEEVGTSGVEGVADRRYAITHAGTDATATPARAAPGLTTVPIVPSAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="63" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LFP8</accession>
    <name>Q1LFP8_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">pelG</name>
        <name evidence="2" type="ordered locus">Rmet_4161</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11028.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518650.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4161"/>
    <dbReference type="EnsemblBacteria" id="ABF11028">
        <property type="protein sequence ID" value="ABF11028"/>
        <property type="gene ID" value="Rmet_4161"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4161"/>
    <dbReference type="eggNOG" id="COG4267">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_043533_1_1_4"/>
    <dbReference type="OMA" id="ITQFQVS"/>
    <dbReference type="OrthoDB" id="859701at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031617">
        <property type="entry name" value="PelG"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16933">
        <property type="entry name" value="PelG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="21"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="66"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="106"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="133"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="163"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="189"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="230"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="273"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="330"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="366"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="396"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="421"/>
            <end position="441"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF11028.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="456" mass="51146" checksum="2F25C4160BED2A3D" modified="2006-05-30" version="1">MAGIGFELRKMLRRDSLSGLIGAYAYAGVISSGPWVLSIVGILLIGLLSVGFVIPGMLITQFQVSVTYLIACSLILTGPMQLSFTRFTSDRLFERRDDLVLSNYHAVALLATMLAGLIGLACAFWAFPEQSVLYRLLMIAGFVIMSNIWIAAIFLSGMKQYKAIVWTFLIGYAITVLAALAMRKSGLEGLLGGFVVGQLCLLVGMITLIYRNYTSRRFMSFEVFQKRYAYPSLIAIGLFYNLGIWLDKFMFWYAPSTGQQMIGPLHASIIYDIPVFLAYLAIIPGMAVFLVRIETDFVEYYDAFYNAVRGGSSLEHIEDMRNTMVQTIRLGLYEIVKVQAIASLVLFVVGGWVLRLLGISELYLPLLYVDVIGASLQVVLLGVLNIYFYLDRRREVLMLTGTFVVLNFVLTRLTLELGPAWYGYGFAVSLLIVVALALYLLDRKLDRLEYETYMLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="72" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LFR2</accession>
    <name>Q1LFR2_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative nucleoside phosphorylase</fullName>
            <ecNumber evidence="2">2.4.2.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_4147</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EC" id="2.4.2.-" evidence="2"/>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11014.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4147"/>
    <dbReference type="EnsemblBacteria" id="ABF11014">
        <property type="protein sequence ID" value="ABF11014"/>
        <property type="gene ID" value="Rmet_4147"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4147"/>
    <dbReference type="eggNOG" id="COG0775">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031248_4_0_4"/>
    <dbReference type="OMA" id="VDMESHV"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009116">
        <property type="term" value="P:nucleoside metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1580">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017831">
        <property type="entry name" value="Hopanoid-assoc_phosphoryl_HpnG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000845">
        <property type="entry name" value="Nucleoside_phosphorylase_d"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035994">
        <property type="entry name" value="Nucleoside_phosphorylase_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01048">
        <property type="entry name" value="PNP_UDP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53167">
        <property type="entry name" value="SSF53167"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03468">
        <property type="entry name" value="HpnG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PNP_UDP_1" evidence="1">
        <location>
            <begin position="26"/>
            <end position="168"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF11014.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="215" mass="21538" checksum="FE09678C88CAB4BA" modified="2006-05-30" version="1">MDFEARIASGAGVETLYGHRDAALARVLDARLAQGCDGVISFGVAGGLDPTLPPGTVIVATAVRAGDASYSTDPYWTAALRRALPQAVCGLLAGSDTPVTTVTGKTALHESHGALAVDMESHIAALAAQTHHIPFAALRVVIDPADRPVPPLAVAGMAADGSTDIGAIIFGLLKAPHQLGGLLRLGRDASAAKSALSAARHAVGAGFALTALAAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LGG0</accession>
    <name>Q1LGG0_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Rmet_3898</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="5">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="5">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PrpD family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10766.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518413.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3898"/>
    <dbReference type="EnsemblBacteria" id="ABF10766">
        <property type="protein sequence ID" value="ABF10766"/>
        <property type="gene ID" value="Rmet_3898"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3898"/>
    <dbReference type="eggNOG" id="COG2079">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026574_3_1_4"/>
    <dbReference type="OMA" id="AKFSMGT"/>
    <dbReference type="OrthoDB" id="688128at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.4100.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036148">
        <property type="entry name" value="MmgE/PrpD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042183">
        <property type="entry name" value="MmgE/PrpD_sf_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042188">
        <property type="entry name" value="MmgE/PrpD_sf_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005656">
        <property type="entry name" value="MmgE_PrpD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045337">
        <property type="entry name" value="MmgE_PrpD_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045336">
        <property type="entry name" value="MmgE_PrpD_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16943">
        <property type="entry name" value="PTHR16943"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03972">
        <property type="entry name" value="MmgE_PrpD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19305">
        <property type="entry name" value="MmgE_PrpD_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103378">
        <property type="entry name" value="SSF103378"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0614">Plasmid</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MmgE_PrpD" evidence="2">
        <location>
            <begin position="11"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="domain" description="MmgE_PrpD_C" evidence="3">
        <location>
            <begin position="273"/>
            <end position="444"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF03972"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF19305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF10766.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="459" mass="48798" checksum="8D91FD64BC08ED58" modified="2006-05-30" version="1">MSDIDTRYPTRQLCEFLARFTLADVPSPVVERTKDLFLDWIASAIAGKDAPAVRRIQEFAAAMGPATGQAEVFVDRRRTSPYFAALINGASSHVVEQDDVHNGSVLHPAAVVFPAVVAAAQAEGKTGAEVLLASIAGYEAGIRIGEFMGRSHYRVFHTTGTVGTLAAAAAVAKLYGLDAEGINQALGSAGTQAAGLWEFLRDAADSKQLHTAKAAADGLQSAWLARAGFTGARQILEGAQGMAAGMSSDANPAALTDGLGTRWATAETSFKFFASCRHTHPAADALKALMLREGLQADQIAAVTAHVHQGAIDVLGPVVNPATIHQAKFSMGTVLGLVAVHAHAGLGEFEQHSLKDAQVSAFRDKVTMELDEEINAAYPRQWIGRVTVRTTDGRTLQARVDVPKGDPDNTLSRPELEAKAIQLGAFRHGATEAEMRAIIARVWQLDREANVNDWLPAAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="72" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LGL2</accession>
    <name>Q1LGL2_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative methyltransferase, ubiE/COQ5 family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_3846</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10714.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518361.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3846"/>
    <dbReference type="EnsemblBacteria" id="ABF10714">
        <property type="protein sequence ID" value="ABF10714"/>
        <property type="gene ID" value="Rmet_3846"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3846"/>
    <dbReference type="eggNOG" id="COG2226">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037990_2_0_4"/>
    <dbReference type="OMA" id="MFFPDRV"/>
    <dbReference type="OrthoDB" id="1664438at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013216">
        <property type="entry name" value="Methyltransf_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08241">
        <property type="entry name" value="Methyltransf_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Methyltransf_11" evidence="1">
        <location>
            <begin position="47"/>
            <end position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF08241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF10714.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="272" mass="28826" checksum="D0391F606024FDF1" modified="2006-05-30" version="1">MTSHGTDTVFAGSVPELYDTLMVPLIFEPYAADLAARAAALAPTHVLEVAAGTGALTRRLASDLPDGTSIVATDLNRPMLDRAAAIGTSRPLTWRQADAMQLPFPDASFDLVICQFGAMFFPEKHKAFAEARRVLKPGGVLLFNVWDRIEENAFAHTVTQTLTGMFPHKPPVFLARTPHGYHDPAVVAADLRLGGFSEASTCATVAAESRAVDANTPAIAFCQGTPLRGELEAFGPDALVNATLACAKALTREFGPGPVSGKIQAHVFSARA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LGR5</accession>
    <name>Q1LGR5_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">L-carnitine dehydratase/bile acid-inducible protein F (CoA-transferase family)</fullName>
            <ecNumber evidence="1">2.8.3.16</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_3791</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EC" id="2.8.3.16" evidence="1"/>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10661.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518309.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3791"/>
    <dbReference type="EnsemblBacteria" id="ABF10661">
        <property type="protein sequence ID" value="ABF10661"/>
        <property type="gene ID" value="Rmet_3791"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3791"/>
    <dbReference type="eggNOG" id="COG1804">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033975_0_0_4"/>
    <dbReference type="OMA" id="RDSAYFG"/>
    <dbReference type="OrthoDB" id="969868at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033608">
        <property type="term" value="F:formyl-CoA transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1540.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10540">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003673">
        <property type="entry name" value="CoA-Trfase_fam_III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044855">
        <property type="entry name" value="CoA-Trfase_III_dom3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023606">
        <property type="entry name" value="CoA-Trfase_III_dom_1_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02515">
        <property type="entry name" value="CoA_transf_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89796">
        <property type="entry name" value="SSF89796"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF10661.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="407" mass="43657" checksum="F084C47E8834D8CF" modified="2006-05-30" version="1">MIKPLSHIRVLDLTRVLAGPWCTQNLADLGAEVIKVERPEVGDETRGWGPPWLPAAEESERRDSSYYASANRGKKSITVNLASADGQAIIRRLAASADVLVENYKVGTLARYGLGYDDLREANPGLVYCSVTGFGQTGPYRHLPGYDFVFQGMGGLMSITGERDDLPGGGPQKVGIAVADITTGMYASLAIAAALTWRERSGQGQHIDMALLDCMLAFNSNQAVSYLVSGKLPKRQGNAHANAVPYQVFDSLDGKLIVAVGNDSQFAAYCRAVGKPELAIDTRFAKVTGRIVNREVLLPMLVDVMRTDSTASWLARLKAAGVPCGPVNDFSQTFEDPQVRHRGIRVDLPLPDGGICPSIASPMRFSATPLEHSAAPPALGEHTDTVLTECLGLLPAELSRLREAGAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="96" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LGY5</accession>
    <name>Q1LGY5_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Transcriptional regulator, LysR family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_3719</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the LysR transcriptional regulatory family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10591.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518238.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3719"/>
    <dbReference type="EnsemblBacteria" id="ABF10591">
        <property type="protein sequence ID" value="ABF10591"/>
        <property type="gene ID" value="Rmet_3719"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3719"/>
    <dbReference type="eggNOG" id="COG0583">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039613_16_4_4"/>
    <dbReference type="OMA" id="LWLICPS"/>
    <dbReference type="OrthoDB" id="1661500at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005119">
        <property type="entry name" value="LysR_subst-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000847">
        <property type="entry name" value="Tscrpt_reg_HTH_LysR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00126">
        <property type="entry name" value="HTH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03466">
        <property type="entry name" value="LysR_substrate"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50931">
        <property type="entry name" value="HTH_LYSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH lysR-type" evidence="2">
        <location>
            <begin position="1"/>
            <end position="59"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF10591.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="302" mass="33664" checksum="FD58C9F93C17FAAA" modified="2006-05-30" version="1">MRLLQELDIFVRTADNGSLSATARALDLTPAATSAALKRLEGELGVQLFVRSTRTMRLTREGEQYLAQCRPALEVLQQASRDLLTGNQAFQGVVRLSAPSDFGRNLLLPWLDAFQDRHPLVEFRLQLTDRMANIYSDPVDVAFRYGEPPDSNLVAVPVATGIHRVLCASPGYLASHDMPQTPYDLSAHRCLCYMFGDDVHDRWRFAREDGETLTVRVKPANVSNDGDAVRRWALAGKGIAYKAWFDVADDLAAGRLRALCTAWTAEASPLYMVVAGRSQLTPLLRGLRDFVAERVERLVARY</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LH71</accession>
    <name>Q1LH71_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Transcriptional activator, TenA family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_3633</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10505.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008646888.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3633"/>
    <dbReference type="EnsemblBacteria" id="ABF10505">
        <property type="protein sequence ID" value="ABF10505"/>
        <property type="gene ID" value="Rmet_3633"/>
    </dbReference>
    <dbReference type="GeneID" id="60826189"/>
    <dbReference type="KEGG" id="rme:Rmet_3633"/>
    <dbReference type="eggNOG" id="COG5424">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1173532_0_0_4"/>
    <dbReference type="OMA" id="ICEIGAQ"/>
    <dbReference type="OrthoDB" id="1571811at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.910.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016084">
        <property type="entry name" value="Haem_Oase-like_multi-hlx"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039068">
        <property type="entry name" value="PqqC-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40279">
        <property type="entry name" value="PTHR40279"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48613">
        <property type="entry name" value="SSF48613"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF10505.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="238" mass="27114" checksum="A51B74B07CFAC9ED" modified="2006-05-30" version="1">MAELMNREEFRTALENAIKGKSANQAPFSKAWASGELSREHLARWAENHYHYVGPFADYLGYIYARTPDHMTEAKDFLLANMYEEEIGGDRHTDLLIRFAEACGTTRERVVSPDNMSPTTRGLQSWCYAVAMREDPVVAVAGLVVGLESQVPSIYRKQTPTLREKYGFTDEEVEFFDLHIVSDEIHGERGYQIVLEHANTPELQQRCLKICEIGAQMRLLYTTALYYDYVDTKAQAAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LHK1</accession>
    <name>Q1LHK1_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Exonuclease involved in chromosome partitioning ParB</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">parB</name>
        <name evidence="5" type="ordered locus">Rmet_3503</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Involved in chromosome partition. Localize to both poles of the predivisional cell following completion of DNA replication. Binds to the DNA origin of replication.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the ParB family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10375.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3503"/>
    <dbReference type="EnsemblBacteria" id="ABF10375">
        <property type="protein sequence ID" value="ABF10375"/>
        <property type="gene ID" value="Rmet_3503"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3503"/>
    <dbReference type="eggNOG" id="COG1475">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023853_0_0_4"/>
    <dbReference type="OMA" id="IALSYQR"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004527">
        <property type="term" value="F:exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041468">
        <property type="entry name" value="HTH_ParB/Spo0J"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004437">
        <property type="entry name" value="ParB/RepB/Spo0J"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003115">
        <property type="entry name" value="ParB/Sulfiredoxin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036086">
        <property type="entry name" value="ParB/Sulfiredoxin_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17762">
        <property type="entry name" value="HTH_ParB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02195">
        <property type="entry name" value="ParBc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00470">
        <property type="entry name" value="ParB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110849">
        <property type="entry name" value="SSF110849"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00180">
        <property type="entry name" value="parB_part"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0540">Nuclease</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ParB" evidence="4">
        <location>
            <begin position="37"/>
            <end position="128"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00025472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00470"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABF10375.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="300" mass="32572" checksum="78655E2C7A7D22F8" modified="2006-05-30" version="1">MATMSTAKKKGLGRGLEALLGGPAEIVETSKQEGAPSVLRVDQLQPGKYQPRTRMDEGALQELAASIRAQGLMQPILVRRVAEPDRYEIIAGERRYRASKLAGLDKVPVLVKDVADEAAAAMALIENIQREDLNPLEEAQGIMRLIREFRFTHEQAAESVGRSRSAVSNLLRLLNLAAPVQTMLMAGDLDMGHARALLAVDGANQITLANQIVNKRLSVRETEKLVASTLKPFDLKSLKQKSGNGTRDVARLEEELSDTLGLSVQIKLGARGKGQLTIHFASNDALDGVLTRLREPQASA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LHM0</accession>
    <name>Q1LHM0_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Bifunctional protein: Zn-dependent hydrolase including glyoxylases (N-terminal) and Rhodanese-related sulfurtransferase (C-terminal)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_3484</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10356.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517913.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3484"/>
    <dbReference type="EnsemblBacteria" id="ABF10356">
        <property type="protein sequence ID" value="ABF10356"/>
        <property type="gene ID" value="Rmet_3484"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3484"/>
    <dbReference type="eggNOG" id="COG0491">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030571_7_0_4"/>
    <dbReference type="OMA" id="DYKGDTV"/>
    <dbReference type="OrthoDB" id="1581636at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016788">
        <property type="term" value="F:hydrolase activity, acting on ester bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050313">
        <property type="term" value="F:sulfur dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006749">
        <property type="term" value="P:glutathione metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07724">
        <property type="entry name" value="POD-like_MBL-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001279">
        <property type="entry name" value="Metallo-B-lactamas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044528">
        <property type="entry name" value="POD-like_MBL-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00753">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00849">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="SSF56281"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Lactamase_B" evidence="1">
        <location>
            <begin position="13"/>
            <end position="180"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF10356.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="248" mass="27120" checksum="92DB597E63B5CFA0" modified="2006-05-30" version="1">MQTFHQLFDETSSTYTYLLIDADTHEAVLIDPVDRQYERDLGVLADTGAKLAWVVETHAHADHITSAGHLAQQTGAHTAAPSGCDIKPAQKQLIDGDTLRFGNQVLRAIHTPGHTAGSMSYLWEEPTTDGVVRRIFTGDALLIDGCGRTDFQSGDAGTLFDSLTQKLFRLPDDTRVYPAHDYKGRTESTIGQERATNSRVAGRTRDEFIVMMRGLNLPRPKLIDVAVPANQQLGLITGRDGESVPHGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LHT4</accession>
    <name>Q1LHT4_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">SoxAX cytochrome complex subunit A</fullName>
            <ecNumber evidence="12">2.8.5.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Protein SoxA</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">Sulfur oxidizing protein A</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">Thiosulfate-oxidizing multienzyme system protein SoxA</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="primary">soxA</name>
        <name evidence="17" type="ordered locus">Rmet_3420</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10 12">
            <text>2 Fe(III)-[cytochrome c] + L-cysteinyl-[SoxY protein] + thiosulfate = 2 Fe(II)-[cytochrome c] + 2 H(+) + S-sulfosulfanyl-L-cysteinyl-[SoxY protein]</text>
            <dbReference type="Rhea" id="RHEA:56720"/>
            <dbReference type="Rhea" id="RHEA-COMP:10350"/>
            <dbReference type="Rhea" id="RHEA-COMP:14328"/>
            <dbReference type="Rhea" id="RHEA-COMP:14399"/>
            <dbReference type="Rhea" id="RHEA-COMP:14691"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:33542"/>
            <dbReference type="ChEBI" id="CHEBI:139321"/>
            <dbReference type="EC" id="2.8.5.2"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11 12">
            <text>2 Fe(III)-[cytochrome c] + S-sulfanyl-L-cysteinyl-[SoxY protein] + thiosulfate = 2 Fe(II)-[cytochrome c] + 2 H(+) + S-(2-sulfodisulfanyl)-L-cysteinyl-[SoxY protein]</text>
            <dbReference type="Rhea" id="RHEA:51224"/>
            <dbReference type="Rhea" id="RHEA-COMP:10350"/>
            <dbReference type="Rhea" id="RHEA-COMP:14399"/>
            <dbReference type="Rhea" id="RHEA-COMP:14689"/>
            <dbReference type="Rhea" id="RHEA-COMP:14690"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
            <dbReference type="ChEBI" id="CHEBI:33542"/>
            <dbReference type="ChEBI" id="CHEBI:61963"/>
            <dbReference type="ChEBI" id="CHEBI:140664"/>
            <dbReference type="EC" id="2.8.5.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="14">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
        <text evidence="14">Binds 2 heme groups per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2 12">Heterodimer of SoxA and SoxX.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 12">Periplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9 12">Belongs to the SoxA family.</text>
    </comment>
    <dbReference type="EC" id="2.8.5.2" evidence="12"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10292.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3420"/>
    <dbReference type="EnsemblBacteria" id="ABF10292">
        <property type="protein sequence ID" value="ABF10292"/>
        <property type="gene ID" value="Rmet_3420"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3420"/>
    <dbReference type="eggNOG" id="COG3258">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_079910_0_0_4"/>
    <dbReference type="OMA" id="RTMQHRL"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070069">
        <property type="term" value="C:cytochrome complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042597">
        <property type="term" value="C:periplasmic space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016669">
        <property type="term" value="F:oxidoreductase activity, acting on a sulfur group of donors, cytochrome as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019417">
        <property type="term" value="P:sulfur oxidation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.760.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036909">
        <property type="entry name" value="Cyt_c-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025710">
        <property type="entry name" value="SoxA"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038455">
        <property type="entry name" value="SoxA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46626">
        <property type="entry name" value="SSF46626"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR04484">
        <property type="entry name" value="thiosulf_SoxA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0249">Electron transport</keyword>
    <keyword evidence="4 12 14" id="KW-0349">Heme</keyword>
    <keyword evidence="8 12 15" id="KW-0408">Iron</keyword>
    <keyword evidence="6 12 15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 12" id="KW-0574">Periplasm</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="16" id="KW-0732">Signal</keyword>
    <keyword evidence="5 12" id="KW-0808">Transferase</keyword>
    <keyword evidence="3 12" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="16">
        <location>
            <begin position="1"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193137" description="SoxAX cytochrome complex subunit A" evidence="16">
        <location>
            <begin position="46"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="active site" description="Cysteine persulfide intermediate" evidence="13">
        <location>
            <position position="252"/>
        </location>
    </feature>
    <feature type="binding site" description="covalent" evidence="14">
        <location>
            <position position="91"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="15">
        <location>
            <position position="127"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
            <label>1</label>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <feature type="binding site" description="covalent" evidence="14">
        <location>
            <position position="200"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" description="covalent" evidence="14">
        <location>
            <position position="203"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="15">
        <location>
            <position position="204"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
            <label>2</label>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="248"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="15">
        <location>
            <position position="252"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
            <label>2</label>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022764"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00025746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00029285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00029308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRNR" id="PIRNR038455"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR038455-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR038455-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR038455-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="ABF10292.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="291" mass="32138" checksum="CE91FA71C323426A" modified="2006-05-30" version="1">MVSPRNTRSNTRRSPRRTYRAAPAVLAVAAAVTAIATTLSAPALAQGSTAEELAKYRQMLAEGNPAELWEAAGEELWKKPAGPKNVSLEQCDLGKGPGVTKGAYAELPRYFKDTNKVMDLEQRLAYCRVTLQGLTQEEATKNPFSSQGKPSDIERLAAYLTGESRGVKMNVQLTHPEEKRVYALGEKMFFYRGGAYDFACATCHAVDGQRIRLQDLPNLLTSKGAEAAYTTWPAYRVSQGEVRTMQHRLYDCLRQQRFPEPAYGSDVITALTMFLAKNANGGTYDGPNMKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LI67</accession>
    <name>Q1LI67_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Putative Mg2+ and Co2+ transporter, CorA-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Rmet_3287</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CorA metal ion transporter (MIT) (TC 1.A.35) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10159.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517760.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3287"/>
    <dbReference type="EnsemblBacteria" id="ABF10159">
        <property type="protein sequence ID" value="ABF10159"/>
        <property type="gene ID" value="Rmet_3287"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3287"/>
    <dbReference type="eggNOG" id="COG0598">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007127_4_0_4"/>
    <dbReference type="OMA" id="SHYDYTS"/>
    <dbReference type="OrthoDB" id="822653at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046873">
        <property type="term" value="F:metal ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045861">
        <property type="entry name" value="CorA_cytoplasmic_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045863">
        <property type="entry name" value="CorA_TM1_TM2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002523">
        <property type="entry name" value="MgTranspt_CorA/ZnTranspt_ZntB"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01544">
        <property type="entry name" value="CorA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143865">
        <property type="entry name" value="SSF143865"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144083">
        <property type="entry name" value="SSF144083"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="333"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="367"/>
            <end position="387"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009765"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABF10159.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="393" mass="44262" checksum="62EA6637FF3607B3" modified="2006-05-30" version="1">MQLLGFSANQVHPLDTLDAARDFLSGNAAARFVWADFGTEEVTPAPSVWRDSLMALTGAPILDLHLADATNPAHPSFFDTTHAYDMVIFRKLTFETSRQFAEAEARQSVAQRKASRKYAPGFLPALARIDTQPVAFFVFDNVLVTVRPSPSRTIEQVRQRLLESCGAPRPASARAGGTAALVHGVRPPTRPEDLMLRLLNAMVDRYLDLRAPLTRQLDRWQRALLSSRHSFSSWEGLLDARIQLRRLEHLSEEQRDALQEFRDSLLDNRYSAEDAEGMQEASRDEVLLVRINDLMEHILRVLAHARRLEDSIESAVQIHFSAVAHRTNRTMRALTVITAVFMPLTLITGIFGMNFARMPWLQEPEGFWWSIGLMGAVVAVIGGVWGLGRWLDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LIA2</accession>
    <name>Q1LIA2_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Transport permease protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Rmet_3252</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cell inner membrane</location>
            <topology evidence="6">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the ABC-2 integral membrane protein family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10124.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517726.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3252"/>
    <dbReference type="DNASU" id="4040087"/>
    <dbReference type="EnsemblBacteria" id="ABF10124">
        <property type="protein sequence ID" value="ABF10124"/>
        <property type="gene ID" value="Rmet_3252"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3252"/>
    <dbReference type="eggNOG" id="COG0842">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039483_3_0_4"/>
    <dbReference type="OMA" id="HSLPPFW"/>
    <dbReference type="OrthoDB" id="1891092at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043190">
        <property type="term" value="C:ATP-binding cassette (ABC) transporter complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013525">
        <property type="entry name" value="ABC_2_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000412">
        <property type="entry name" value="ABC_2_transport"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01061">
        <property type="entry name" value="ABC2_membrane"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006648">
        <property type="entry name" value="DrrB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00164">
        <property type="entry name" value="ABC2TRNSPORT"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51012">
        <property type="entry name" value="ABC_TM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4 5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="40"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="75"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="124"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="155"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="193"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="244"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="domain" description="ABC2_membrane" evidence="7">
        <location>
            <begin position="26"/>
            <end position="236"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00442"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU361157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ABF10124.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="273" mass="30163" checksum="A35B7468033404A9" modified="2006-05-30" version="1">MKNPAEIDVADQQRLESMAVGGLIGFRTLLYKEVLRFWKVSFQTVAAPVLTAVLYLLIFGHVLEDRVRVYDQISYTAFLVPGLVMMSVLQNAFANSSSSLIQSKITGNLVFILLPPLSHWEMFGAYVVASIIRGLCVGLGVLLVTAWFANLHFAHIGWILVFGLMGAGILGTLGLIAGIWAEKFDQLAAFQNFLIMPATFLSGVFYSIHSLPAFWQAVSHANPFFYMIDGFRYGFFGVSDVPPLQSLAVVVVAFVVLAALALRLLRNGYKLRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LIC7</accession>
    <name>Q1LIC7_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Stringent starvation protein A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">sspA</name>
        <name evidence="4" type="ordered locus">Rmet_3227</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the GST superfamily. HSP26 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10099.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008643086.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3227"/>
    <dbReference type="EnsemblBacteria" id="ABF10099">
        <property type="protein sequence ID" value="ABF10099"/>
        <property type="gene ID" value="Rmet_3227"/>
    </dbReference>
    <dbReference type="GeneID" id="60825551"/>
    <dbReference type="KEGG" id="rme:Rmet_3227"/>
    <dbReference type="eggNOG" id="COG0625">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011226_9_3_4"/>
    <dbReference type="OMA" id="ADHYSHR"/>
    <dbReference type="OrthoDB" id="1997747at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006749">
        <property type="term" value="P:glutathione metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03059">
        <property type="entry name" value="GST_N_SspA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010987">
        <property type="entry name" value="Glutathione-S-Trfase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036282">
        <property type="entry name" value="Glutathione-S-Trfase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040079">
        <property type="entry name" value="Glutathione_S-Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004045">
        <property type="entry name" value="Glutathione_S-Trfase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004046">
        <property type="entry name" value="GST_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034341">
        <property type="entry name" value="SspA_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00043">
        <property type="entry name" value="GST_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13417">
        <property type="entry name" value="GST_N_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00019">
        <property type="entry name" value="Glutathione_Transferase_(cytos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47616">
        <property type="entry name" value="SSF47616"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="SSF52833"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50405">
        <property type="entry name" value="GST_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50404">
        <property type="entry name" value="GST_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GST N-terminal" evidence="2">
        <location>
            <begin position="1"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="domain" description="GST C-terminal" evidence="3">
        <location>
            <begin position="83"/>
            <end position="203"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF10099.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="203" mass="23788" checksum="F231532B5392AF5A" modified="2006-05-30" version="1">MMVLYSGTTCPFSQRCRLVLFEKGMDFEIRDVDLFNKPEDISVMNPYGQVPILVERDLILYESNIINEYIDERFPHPQLMPADPVQRARARLFLFNFEKELFTHVYVLENEKGKAAEKNHERARAAIRDRLTQLAPIFVKNKYMLGEEFSMLDVAIAPLLWRLDHYGIELSKNAAPLLKYAERIFSRPAYIEALTPSEKVMRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="100" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LIG1</accession>
    <name>Q1LIG1_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ribosome-binding ATPase YchF</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4 7" type="primary">ychF</name>
        <name evidence="7" type="ordered locus">Rmet_3193</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">ATPase that binds to both the 70S ribosome and the 50S ribosomal subunit in a nucleotide-independent manner.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the TRAFAC class OBG-HflX-like GTPase superfamily. OBG GTPase family. YchF/OLA1 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10065.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517674.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3193"/>
    <dbReference type="EnsemblBacteria" id="ABF10065">
        <property type="protein sequence ID" value="ABF10065"/>
        <property type="gene ID" value="Rmet_3193"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3193"/>
    <dbReference type="eggNOG" id="COG0012">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018395_0_1_4"/>
    <dbReference type="OMA" id="VLRCFDN"/>
    <dbReference type="OrthoDB" id="603226at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043023">
        <property type="term" value="F:ribosomal large subunit binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043022">
        <property type="term" value="F:ribosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd04867">
        <property type="entry name" value="TGS_YchF_OLA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01900">
        <property type="entry name" value="YchF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00944">
        <property type="entry name" value="YchF_OLA1_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004396">
        <property type="entry name" value="ATPase_YchF/OLA1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012675">
        <property type="entry name" value="Beta-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031167">
        <property type="entry name" value="G_OBG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004095">
        <property type="entry name" value="TGS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012676">
        <property type="entry name" value="TGS-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023192">
        <property type="entry name" value="TGS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013029">
        <property type="entry name" value="YchF_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041706">
        <property type="entry name" value="YchF_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01926">
        <property type="entry name" value="MMR_HSR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06071">
        <property type="entry name" value="YchF-GTPase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006641">
        <property type="entry name" value="CHP00092"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00326">
        <property type="entry name" value="GTP1OBG"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81271">
        <property type="entry name" value="SSF81271"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00092">
        <property type="entry name" value="TIGR00092"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51710">
        <property type="entry name" value="G_OBG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51880">
        <property type="entry name" value="TGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0460">Magnesium</keyword>
    <keyword evidence="1 4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="OBG-type G" evidence="5">
        <location>
            <begin position="3"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="domain" description="TGS" evidence="6">
        <location>
            <begin position="278"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="12"/>
            <end position="17"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00944"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51710"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABF10065.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="363" mass="39211" checksum="43EDECA2B3EFCEE3" modified="2006-05-30" version="1">MSLKCGIVGLPNVGKSTLFNALTKAGIAAENYPFCTIEPNVGVVEVPDPRLAKLAEIVKPERILPATVEFVDIAGLVAGASKGEGLGNQFLANIRETDAITHVVRCFEDDNVIHVAGKVDPLSDIEVINTELALADLATVEKALARYIKPARAGDKEALRLVAVLEKAQAVLDQAKAVRTLDLAPEEWEALRPFCLITAKPTMYVANVREDGFENNPHLDAVREYAKTTGSPVVAVCAAIEAEIADLDDADKAEFLADLGMEEPGLDRVIRAGFKLLGLQTYFTAGVKEVRAWTIHVGDTAPKAAGVIHTDFERGFIRAQTIAYDDFIAFKGEHGAKEAGKMRAEGKEYVVHDGDVMNFLFNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LIJ1</accession>
    <name>Q1LIJ1_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Acyl-CoA hydratase</fullName>
            <ecNumber evidence="3">4.2.1.17</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">paaG</name>
        <name evidence="3" type="ordered locus">Rmet_3163</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the enoyl-CoA hydratase/isomerase family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.17" evidence="3"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10035.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3163"/>
    <dbReference type="EnsemblBacteria" id="ABF10035">
        <property type="protein sequence ID" value="ABF10035"/>
        <property type="gene ID" value="Rmet_3163"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3163"/>
    <dbReference type="eggNOG" id="COG1024">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009834_7_2_4"/>
    <dbReference type="OMA" id="LDCEKPV"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004300">
        <property type="term" value="F:enoyl-CoA hydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010124">
        <property type="term" value="P:phenylacetate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.12.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029045">
        <property type="entry name" value="ClpP/crotonase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001753">
        <property type="entry name" value="Enoyl-CoA_hydra/iso"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014748">
        <property type="entry name" value="Enoyl-CoA_hydra_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011968">
        <property type="entry name" value="PaaB1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00378">
        <property type="entry name" value="ECH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52096">
        <property type="entry name" value="SSF52096"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02280">
        <property type="entry name" value="PaaB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0456">Lyase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF10035.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="280" mass="29731" checksum="396133B5FA982132" modified="2006-05-30" version="1">MAQETTMPPSQAQTTARASLDSGPILLHWQGDVAVVTLNRPDKLNSFTRDMHRALVTALDHVEAGGARALLLTGSGRGFCAGQDLADLDFTPGQATDLGELINTWFNPLVRRLQALPLPVIAAVNGTAAGAGANLAFACDIVLAARSASFIQAFVKIGLLPDSGGTWLLPKRVGMARALGLAMTGDKLSAEEAAKWGLVWEVMDDPLLAEQAMALATHLSAQPTRALAAIKQAIHASATNTYDAQLDLERDLQRELGQSHDYNEGVNAFLEKRAPRFTGA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-02-26" modified="2022-08-03" version="102" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LIN6</accession>
    <name>SECA_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Protein translocase subunit SecA</fullName>
            <ecNumber evidence="1">7.4.2.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">secA</name>
        <name type="ordered locus">Rmet_3118</name>
    </gene>
    <organism>
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Part of the Sec protein translocase complex. Interacts with the SecYEG preprotein conducting channel. Has a central role in coupling the hydrolysis of ATP to the transfer of proteins into and across the cell membrane, serving both as a receptor for the preprotein-SecB complex and as an ATP-driven molecular motor driving the stepwise translocation of polypeptide chains across the membrane.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + H2O + cellular proteinSide 1 = ADP + phosphate + cellular proteinSide 2.</text>
            <dbReference type="EC" id="7.4.2.8"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="1">May bind 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Monomer and homodimer. Part of the essential Sec protein translocation apparatus which comprises SecA, SecYEG and auxiliary proteins SecDF-YajC and YidC.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell inner membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
        <text evidence="1">Distribution is 50-50.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the SecA family.</text>
    </comment>
    <dbReference type="EC" id="7.4.2.8" evidence="1"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09990.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517613.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q1LIN6"/>
    <dbReference type="SMR" id="Q1LIN6"/>
    <dbReference type="STRING" id="266264.Rmet_3118"/>
    <dbReference type="EnsemblBacteria" id="ABF09990">
        <property type="protein sequence ID" value="ABF09990"/>
        <property type="gene ID" value="Rmet_3118"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3118"/>
    <dbReference type="eggNOG" id="COG0653">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005314_3_0_4"/>
    <dbReference type="OMA" id="MVHYDVQ"/>
    <dbReference type="OrthoDB" id="212453at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008564">
        <property type="term" value="F:protein-exporting ATPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0065002">
        <property type="term" value="P:intracellular protein transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017038">
        <property type="term" value="P:protein import"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006605">
        <property type="term" value="P:protein targeting"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd18803">
        <property type="entry name" value="SF2_C_secA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01382">
        <property type="entry name" value="SecA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004027">
        <property type="entry name" value="SEC_C_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000185">
        <property type="entry name" value="SecA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020937">
        <property type="entry name" value="SecA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011115">
        <property type="entry name" value="SecA_DEAD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014018">
        <property type="entry name" value="SecA_motor_DEAD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011130">
        <property type="entry name" value="SecA_preprotein_X-link_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044722">
        <property type="entry name" value="SecA_SF2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011116">
        <property type="entry name" value="SecA_Wing/Scaffold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036266">
        <property type="entry name" value="SecA_Wing/Scaffold_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036670">
        <property type="entry name" value="SecA_X-link_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30612">
        <property type="entry name" value="PTHR30612"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02810">
        <property type="entry name" value="SEC-C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07517">
        <property type="entry name" value="SecA_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01043">
        <property type="entry name" value="SecA_PP_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07516">
        <property type="entry name" value="SecA_SW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00906">
        <property type="entry name" value="SECA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00957">
        <property type="entry name" value="SecA_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00958">
        <property type="entry name" value="SecA_PP_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81767">
        <property type="entry name" value="SSF81767"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81886">
        <property type="entry name" value="SSF81886"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00963">
        <property type="entry name" value="secA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01312">
        <property type="entry name" value="SECA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51196">
        <property type="entry name" value="SECA_MOTOR_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0997">Cell inner membrane</keyword>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0653">Protein transport</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-1278">Translocase</keyword>
    <keyword id="KW-0811">Translocation</keyword>
    <keyword id="KW-0813">Transport</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <feature type="chain" id="PRO_0000320912" description="Protein translocase subunit SecA">
        <location>
            <begin position="1"/>
            <end position="930"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="87"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="105"/>
            <end position="109"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="515"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="914"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="916"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="925"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="926"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01382"/>
        </source>
    </evidence>
    <sequence length="930" mass="105159" checksum="7AFCE4E076B3107D" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="61" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LIX9</accession>
    <name>Q1LIX9_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Replication initiator and transcription repressor protein RepA (Maintenance module of genomic island family Tn4371)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">repA</name>
        <name evidence="1" type="ordered locus">Rmet_3024</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09897.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517539.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3024"/>
    <dbReference type="EnsemblBacteria" id="ABF09897">
        <property type="protein sequence ID" value="ABF09897"/>
        <property type="gene ID" value="Rmet_3024"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3024"/>
    <dbReference type="eggNOG" id="COG5534">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_050846_0_0_4"/>
    <dbReference type="OMA" id="ERRMHRF"/>
    <dbReference type="OrthoDB" id="355274at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018777">
        <property type="entry name" value="Replication_initiator_prot_A"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10134">
        <property type="entry name" value="RPA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF09897.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="285" mass="32268" checksum="8CA8604F474D080F" modified="2006-05-30" version="1">MSSPALPMRQRPMQEREQLDLFRALPGDMAPRDSQDLMAFPFFSLAKSRRTAPIDFRASGITIRVEGTQEHGIATIWDADILIWAASQIVEARDAGLRPSRLMQATPYEILRFIGRGTSLRDYQRLKAALDRLQSTTVATSIRETTGRRLHRFSWINEWKELADAKGTPLGLELILPDWFYAGVLDAALVLTIDPAYFRLTGGIERWLYRLVRKHGGRQPGGWQFDFQHLYRKSGSVARYYDFAADLRALVVRQALPGYQLGIEYVSGIASPLLTFRPVPSTARG</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="99" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LJ15</accession>
    <name>Q1LJ15_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Methylenetetrahydrofolate reductase</fullName>
            <ecNumber evidence="8">1.5.1.20</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">metF2</name>
        <name evidence="9" type="ordered locus">Rmet_2988</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="2 8">
            <text>(6S)-5-methyl-5,6,7,8-tetrahydrofolate + NAD(+) = (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate + H(+) + NADH</text>
            <dbReference type="Rhea" id="RHEA:19821"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15636"/>
            <dbReference type="ChEBI" id="CHEBI:18608"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.5.1.20"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 8">
            <text>(6S)-5-methyl-5,6,7,8-tetrahydrofolate + NADP(+) = (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate + H(+) + NADPH</text>
            <dbReference type="Rhea" id="RHEA:19817"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15636"/>
            <dbReference type="ChEBI" id="CHEBI:18608"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.5.1.20"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3 8">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="4 8">One-carbon metabolism; tetrahydrofolate interconversion.</text>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the methylenetetrahydrofolate reductase family.</text>
    </comment>
    <dbReference type="EC" id="1.5.1.20" evidence="8"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09861.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_003056080.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2988"/>
    <dbReference type="EnsemblBacteria" id="ABF09861">
        <property type="protein sequence ID" value="ABF09861"/>
        <property type="gene ID" value="Rmet_2988"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2988"/>
    <dbReference type="eggNOG" id="COG0685">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_081788_0_0_4"/>
    <dbReference type="OMA" id="VYIAHIE"/>
    <dbReference type="OrthoDB" id="1425269at2"/>
    <dbReference type="UniPathway" id="UPA00193"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106312">
        <property type="term" value="F:methylenetetrahydrofolate reductase NADH activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106313">
        <property type="term" value="F:methylenetetrahydrofolate reductase NADPH activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006555">
        <property type="term" value="P:methionine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035999">
        <property type="term" value="P:tetrahydrofolate interconversion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00537">
        <property type="entry name" value="MTHFR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029041">
        <property type="entry name" value="FAD-linked_oxidoreductase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003171">
        <property type="entry name" value="Mehydrof_redctse-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02219">
        <property type="entry name" value="MTHFR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51730">
        <property type="entry name" value="SSF51730"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0274">FAD</keyword>
    <keyword evidence="5 8" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="7 8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000431"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001287"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ABF09861.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="300" mass="31861" checksum="F2C26FD4A2BEBB7A" modified="2006-05-30" version="1">MASPLLHSDKSKPIGNITDAYSLEVNAKDISALTAAAPRVLPGSTISIPYLANQDNDARLAAAQAVRGLGFEPMPHLAARRIASFAKLESFIQRAVAEAGVKRCFVIAGDPSTPLGPFADSSSLIESGVFERSGIQMVGVGGHPEGHPVMSTSDRWKVLEHKCRCIEGRGMAPLIVTQFAFDADIVLAWLEALRGRGVEHPVRVGVPGPAGVAVLARYAALCGVSACASMLSKYGISISKLFGIAGPDLFVDRLTTRLTEAHGKVSLHFFPFGGIAQSVKWVEQYCSRAEPEQPIDTAPM</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="98" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LJ62</accession>
    <name>Q1LJ62_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Oxidative stress-inducible genes activator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">oxyR</name>
        <name evidence="3" type="ordered locus">Rmet_2941</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the LysR transcriptional regulatory family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09814.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517481.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2941"/>
    <dbReference type="EnsemblBacteria" id="ABF09814">
        <property type="protein sequence ID" value="ABF09814"/>
        <property type="gene ID" value="Rmet_2941"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2941"/>
    <dbReference type="eggNOG" id="COG0583">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039613_6_4_4"/>
    <dbReference type="OMA" id="DQALDIC"/>
    <dbReference type="OrthoDB" id="990666at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005119">
        <property type="entry name" value="LysR_subst-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000847">
        <property type="entry name" value="Tscrpt_reg_HTH_LysR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00126">
        <property type="entry name" value="HTH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03466">
        <property type="entry name" value="LysR_substrate"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00039">
        <property type="entry name" value="HTHLYSR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50931">
        <property type="entry name" value="HTH_LYSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH lysR-type" evidence="2">
        <location>
            <begin position="1"/>
            <end position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF09814.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="318" mass="34982" checksum="6470D82369A2B92C" modified="2006-05-30" version="1">MTLTELKYIVAVARERHFGRAAEACFVSQPTLSVAIKKLEDELTVQIFERGTSEVSVTAIGEQIVAQAQRVLEQTMAIREIAKQGKDPLAGPLRLGVIYTIGPYLLPALVKQMIETVPQMPLMLQENYTNKLIELLKQGEIDCAIMAEPFPDSGLTVRPLYDEPFVVAVPRGHQLSQQTKVDPDELKQQTMLLLGSGHCFRDHVLSVCPELSRFSQAADGIQKTFEGSSLETIRHMVASGVGITVLPRTSVPDLKPHNDMLSYVPFAEPVPDRRVVLAWRKSFTRLPAMEALANAVAACELPGVRRLGDTETVEAVAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LJ66</accession>
    <name>Q1LJ66_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Pyridoxal phosphate homeostasis protein</fullName>
            <shortName evidence="1">PLP homeostasis protein</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">yggS</name>
        <name evidence="5" type="ordered locus">Rmet_2937</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Pyridoxal 5'-phosphate (PLP)-binding protein, which is involved in PLP homeostasis.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the pyridoxal phosphate-binding protein YggS/PROSC family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09810.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517478.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2937"/>
    <dbReference type="EnsemblBacteria" id="ABF09810">
        <property type="protein sequence ID" value="ABF09810"/>
        <property type="gene ID" value="Rmet_2937"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2937"/>
    <dbReference type="eggNOG" id="COG0325">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_059988_0_1_4"/>
    <dbReference type="OMA" id="IEWHMIG"/>
    <dbReference type="OrthoDB" id="1423012at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02087">
        <property type="entry name" value="PLP_homeostasis"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001608">
        <property type="entry name" value="Ala_racemase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029066">
        <property type="entry name" value="PLP-binding_barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011078">
        <property type="entry name" value="PyrdxlP_homeostasis"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10146">
        <property type="entry name" value="PTHR10146"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01168">
        <property type="entry name" value="Ala_racemase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004848">
        <property type="entry name" value="YBL036c_PLPDEIII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51419">
        <property type="entry name" value="SSF51419"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00044">
        <property type="entry name" value="TIGR00044"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01211">
        <property type="entry name" value="UPF0001"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 2" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ala_racemase_N" evidence="4">
        <location>
            <begin position="7"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="1 2">
        <location>
            <position position="36"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR004848-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABF09810.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="229" mass="24364" checksum="FC294A2FB4C18AF2" modified="2006-05-30" version="1">MSVIAANLQAVKNDIAAAAQQASRDPSGVTLLAVSKTVSPDRVREAFEAGQPAFGENYVQEGLDKIAALGDLRSRIAWHFIGPLQSNKTRPVAEQFDWVHAIDRLKIAERLSAQRPADLAPLQVCIQVNISNEDTKSGVLPEEVLALARAVAALPNLRLRGLMAIPAPAADLAAQRVPFAALRRLLEELRQSGLDVDTLSMGMSADMPAAIAEGATMVRIGTAIFGARG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-11-28" modified="2022-05-25" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LJ99</accession>
    <name>RS20_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">30S ribosomal protein S20</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpsT</name>
        <name type="ordered locus">Rmet_2904</name>
    </gene>
    <organism>
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Binds directly to 16S ribosomal RNA.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the bacterial ribosomal protein bS20 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09777.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008644962.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q1LJ99"/>
    <dbReference type="SMR" id="Q1LJ99"/>
    <dbReference type="STRING" id="266264.Rmet_2904"/>
    <dbReference type="EnsemblBacteria" id="ABF09777">
        <property type="protein sequence ID" value="ABF09777"/>
        <property type="gene ID" value="Rmet_2904"/>
    </dbReference>
    <dbReference type="GeneID" id="60820660"/>
    <dbReference type="KEGG" id="rme:Rmet_2904"/>
    <dbReference type="eggNOG" id="COG0268">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_160655_4_0_4"/>
    <dbReference type="OMA" id="GVIHKNA"/>
    <dbReference type="OrthoDB" id="2005443at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00500">
        <property type="entry name" value="Ribosomal_S20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002583">
        <property type="entry name" value="Ribosomal_S20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036510">
        <property type="entry name" value="Ribosomal_S20_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33398">
        <property type="entry name" value="PTHR33398"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01649">
        <property type="entry name" value="Ribosomal_S20p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46992">
        <property type="entry name" value="SSF46992"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00029">
        <property type="entry name" value="S20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <feature type="chain" id="PRO_0000260132" description="30S ribosomal protein S20">
        <location>
            <begin position="1"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00500"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="88" mass="9403" checksum="B1E5485B4CFC8AE5" modified="2006-05-30" version="1">MANSAQARKRARQAVAANAHNSSLRSRLRTAVKAVRKAIDAGDKAAAAEIYKQSQVVIDSIADKKIVHKNKAARHKSRLSAAIKAMAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-10-07" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LJB2</accession>
    <name>Q1LJB2_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Extra-cytoplasmic Solute Receptor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">bug</name>
        <name evidence="4" type="ordered locus">Rmet_2891</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Periplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the UPF0065 (bug) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09764.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2891"/>
    <dbReference type="EnsemblBacteria" id="ABF09764">
        <property type="protein sequence ID" value="ABF09764"/>
        <property type="gene ID" value="Rmet_2891"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2891"/>
    <dbReference type="eggNOG" id="COG3181">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045683_0_1_4"/>
    <dbReference type="OMA" id="KANIQFE"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042597">
        <property type="term" value="C:periplasmic space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005064">
        <property type="entry name" value="BUG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042100">
        <property type="entry name" value="Bug_dom1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42928">
        <property type="entry name" value="PTHR42928"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03401">
        <property type="entry name" value="TctC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017082">
        <property type="entry name" value="YflP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0675">Receptor</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193549" evidence="3">
        <location>
            <begin position="34"/>
            <end position="334"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF09764.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="334" mass="35195" checksum="F21A3A95E51DF8AF" modified="2006-05-30" version="1">MSSPDRMRLRRLLLKTLPMGAVPVASISSLARAAEPASGGRPISMVLPFPPGGSVDIVARQLQPGLKSGLGQTVVVDNKPGAGGLIASSTVARAKPDGNTLLMAFDTHAINPFAYKNLPYDTFKDFTPISQLVRFPLVIAANPALSASNVRELVELARANPKGVRYASSGIGSLNQLAAEALATDAGVQFLHVPYKGGGPAVQAVLAGEVDLFFSSYAAVQAHIAAKTIKVLGVTGSSRIKQLPQVPTVAEQGFKGFEAYSWIGVFGPAHMQPDTVTRLHDALGVAIRQPKVVEALTAQGFEIAVGTPADLGNLVRHEHDKWQAVARKANIQFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LK49</accession>
    <name>Q1LK49_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Regulatory protein, IclR family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Rmet_2600</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09477.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517180.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2600"/>
    <dbReference type="EnsemblBacteria" id="ABF09477">
        <property type="protein sequence ID" value="ABF09477"/>
        <property type="gene ID" value="Rmet_2600"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2600"/>
    <dbReference type="eggNOG" id="COG1414">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_062618_3_1_4"/>
    <dbReference type="OMA" id="GWYEAHD"/>
    <dbReference type="OrthoDB" id="370329at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029016">
        <property type="entry name" value="GAF-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014757">
        <property type="entry name" value="Tscrpt_reg_IclR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005471">
        <property type="entry name" value="Tscrpt_reg_IclR_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09339">
        <property type="entry name" value="HTH_IclR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01614">
        <property type="entry name" value="IclR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00346">
        <property type="entry name" value="HTH_ICLR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51077">
        <property type="entry name" value="HTH_ICLR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51078">
        <property type="entry name" value="ICLR_ED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH iclR-type" evidence="3">
        <location>
            <begin position="5"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="domain" description="IclR-ED" evidence="4">
        <location>
            <begin position="68"/>
            <end position="249"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABF09477.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="249" mass="27426" checksum="2CDBE50160163455" modified="2006-05-30" version="1">MPSFVPAAARSLAVFEVFSREKRELSNSELARLLDLPESSCSDLLHTLTETGYLMRTARTRRFYPTMRLHSICEAIAGNDPLAAVAAEATEFLTGKTGETSLCGRLDGAYVRVTGFQESRYELRYVLRVGKRLALHATALGKALLAVLPPEDAARQLRLRALPKLGPKTEVNPVALEKQVAEVRERGWAWVEDEGGEGVTAIAVAGRIGDEPFAISLAGPTERFNRRKDEYLEVIEAARDLVFPTAQKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="59" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LK57</accession>
    <name>Q1LK57_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_2592</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09469.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2592"/>
    <dbReference type="EnsemblBacteria" id="ABF09469">
        <property type="protein sequence ID" value="ABF09469"/>
        <property type="gene ID" value="Rmet_2592"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2592"/>
    <dbReference type="eggNOG" id="ENOG5033C2R">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_091686_0_0_4"/>
    <dbReference type="OMA" id="VERMMHN"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="105"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="244"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="105"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="135"/>
            <end position="187"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF09469.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="265" mass="29239" checksum="C43F3C6C675D97F7" modified="2006-05-30" version="1">MAFMVARTLSARTDMQSFVTLREMRRNTAYRPGVVCLRSCRINAGKGDVPIRNDGPNTLTTYVEESIMKRNSVLLAGLIAATLSGIASAQTGAVTYDTQRDVNQQQRIENGLKSGTLTTREASSLEKQESKVDRMEANSLQNGRLSPQEQARIQNAQNRVSQDISRDTHNGKVGNPDSVSSQRMQADVQRNINQETRVRNGVENGSLTNRETAQLERGQAHVDHAEARAGRNGHVGAGEQRSINRAENHASGQIYHDKHNGHRRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LKQ4</accession>
    <name>Q1LKQ4_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Putative thiol:disulfide interchange protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">dsbE</name>
        <name evidence="8" type="ordered locus">Rmet_2395</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the Lgt family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09272.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004350666.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2395"/>
    <dbReference type="EnsemblBacteria" id="ABF09272">
        <property type="protein sequence ID" value="ABF09272"/>
        <property type="gene ID" value="Rmet_2395"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2395"/>
    <dbReference type="eggNOG" id="COG0526">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG0682">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_089636_0_0_4"/>
    <dbReference type="OMA" id="LAYWPMY"/>
    <dbReference type="OrthoDB" id="1617952at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005887">
        <property type="term" value="C:integral component of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015036">
        <property type="term" value="F:disulfide oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008961">
        <property type="term" value="F:phosphatidylglycerol-prolipoprotein diacylglyceryl transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017004">
        <property type="term" value="P:cytochrome complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042158">
        <property type="term" value="P:lipoprotein biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001640">
        <property type="entry name" value="Lgt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013740">
        <property type="entry name" value="Redoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017937">
        <property type="entry name" value="Thioredoxin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01790">
        <property type="entry name" value="LGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08534">
        <property type="entry name" value="Redoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="SSF52833"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00194">
        <property type="entry name" value="THIOREDOXIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0201">Cytochrome c-type biogenesis</keyword>
    <keyword evidence="4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="6"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="37"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="81"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="109"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="domain" description="Thioredoxin" evidence="7">
        <location>
            <begin position="131"/>
            <end position="268"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ABF09272.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="278" mass="30460" checksum="DB559EC5926F5B7E" modified="2006-05-30" version="1">MISVGPFSVQVVAVFLAVLLAWAVARMVAKRLPDSPYKAAGGMLLDAAFVGFVAARLGYIAQWWEEYAQSPMSMISIGDQGFSWWVGVLAALALIWWRTRAVRALRRPVLAGVAVGLAAWFATGGVLALLQRSAPPLPALTLATLDEQPVVLNSYAGRPVVLNLWASWCPPCRREMPVFEQAQAQYPDIAFVMVNQGESAQQARAFLESERLHLKDVLLDPASQTMQAVASRGLPTTLFFDEQGRLVDTHLGELTMASLKHTVSRRFAPAQQIKTDKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-04-07" version="59" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LKW7</accession>
    <name>Q1LKW7_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Conserved putative exported protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Rmet_2332</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09209.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_003098888.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2332"/>
    <dbReference type="EnsemblBacteria" id="ABF09209">
        <property type="protein sequence ID" value="ABF09209"/>
        <property type="gene ID" value="Rmet_2332"/>
    </dbReference>
    <dbReference type="GeneID" id="56409839"/>
    <dbReference type="KEGG" id="rme:Rmet_2332"/>
    <dbReference type="eggNOG" id="ENOG502Z87B">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_108020_1_0_4"/>
    <dbReference type="OMA" id="WFLIYAW"/>
    <dbReference type="OrthoDB" id="1808854at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="20"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="123"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="44"/>
            <end position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF09209.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="214" mass="22927" checksum="DF0921FB548316CC" modified="2006-05-30" version="1">MNAPQPAQRSTAAVVVQSLMWLWLIFLSVLAALGYQALSDQADQERLDSRLQRLEAQATGLAETIEAIQQRPAVATDADLKDTRQILEARAAQVEKSLSGYAAADDLQALRAEVEQIKARQAAARATAPAQRRASRTSAASKTELPPLPFRVVGAELRAGQRSVSVAPSIGDFTPAQLQVLLPGDAVGPWRLQAIEGNTAVFQAGNQTRRVAIP</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="67" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LL42</accession>
    <name>Q1LL42_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Cellulose biosynthesis protein, putative</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_2255</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09134.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516957.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2255"/>
    <dbReference type="EnsemblBacteria" id="ABF09134">
        <property type="protein sequence ID" value="ABF09134"/>
        <property type="gene ID" value="Rmet_2255"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2255"/>
    <dbReference type="eggNOG" id="COG0455">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037612_7_0_4"/>
    <dbReference type="OMA" id="TAWHAST"/>
    <dbReference type="OrthoDB" id="729012at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017746">
        <property type="entry name" value="Cellulose_synthase_operon_BcsQ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06564">
        <property type="entry name" value="CBP_BcsQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03371">
        <property type="entry name" value="cellulose_yhjQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF09134.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="251" mass="26549" checksum="2BF55B3FD5AC293C" modified="2006-05-30" version="1">MNTVAIVSTTGGAGRSTLTAELASLLAQRKHPALALECDPANVLGFHFGMREIPEDGLGAYLDAPTPGAWARAGQRSDDDVLFVPWGSGGAPAALANAAPDWLGRLLAQVDLPPRGVTLIDCARWPSPLADQAIAAADLVLVLAPAQPETCLTLRRLADALTAQGKTARYVATRLQPARQLHVDIVALLQAMLGQDMLPYHVHDDSSVAEALARSESFCRSTPHSQAAHDMNGLASWLSAWLEAATQESRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LLA7</accession>
    <name>Q1LLA7_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">RNA-binding protein, containing KH domain</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Rmet_2190</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09069.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516897.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2190"/>
    <dbReference type="EnsemblBacteria" id="ABF09069">
        <property type="protein sequence ID" value="ABF09069"/>
        <property type="gene ID" value="Rmet_2190"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2190"/>
    <dbReference type="eggNOG" id="COG1534">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_095994_0_0_4"/>
    <dbReference type="OMA" id="VIWKPEA"/>
    <dbReference type="OrthoDB" id="2034603at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.110.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001890">
        <property type="entry name" value="RNA-binding_CRM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035920">
        <property type="entry name" value="YhbY-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01985">
        <property type="entry name" value="CRS1_YhbY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01103">
        <property type="entry name" value="CRS1_YhbY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75471">
        <property type="entry name" value="SSF75471"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51295">
        <property type="entry name" value="CRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="CRM" evidence="4">
        <location>
            <begin position="4"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="104"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="124"/>
            <end position="151"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00626"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABF09069.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="169" mass="18098" checksum="D2C3C8D8590C6105" modified="2006-05-30" version="1">MPALQLISAQRSDLRSRAHSLNPVVMIGAEGLTPAVLAEIDRSLAAHDLIKIRVLGDERDARIAIYDAICDELSAAPIQHIGKLLVIWRPGPALLKENQPEELGAAARRRATGTGAAPRTVVVRKPSTSGQRRPKSTAVTVLGNERVTQGGNVKRSRARPASQKKKALG</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LLF6</accession>
    <name>Q1LLF6_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Predicted esterase of the alpha-beta hydrolase superfamily</fullName>
            <ecNumber evidence="4">3.1.-.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Rmet_2141</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="3.1.-.-" evidence="4"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09020.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516849.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF09020">
        <property type="protein sequence ID" value="ABF09020"/>
        <property type="gene ID" value="Rmet_2141"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2141"/>
    <dbReference type="eggNOG" id="COG1752">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047251_1_1_4"/>
    <dbReference type="OMA" id="LQTFAIM"/>
    <dbReference type="OrthoDB" id="373926at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016035">
        <property type="entry name" value="Acyl_Trfase/lysoPLipase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002641">
        <property type="entry name" value="PNPLA_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01734">
        <property type="entry name" value="Patatin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52151">
        <property type="entry name" value="SSF52151"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51635">
        <property type="entry name" value="PNPLA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="1 2" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PNPLA" evidence="3">
        <location>
            <begin position="50"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="GXSXG" evidence="2">
        <location>
            <begin position="81"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="DGA/G" evidence="2">
        <location>
            <begin position="199"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="2">
        <location>
            <position position="83"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="2">
        <location>
            <position position="199"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF09020.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="316" mass="32562" checksum="73DA2BBABD9FD37E" modified="2006-05-30" version="1">MQRRNFLGASAAMLLAACSFGPKPDATNPGTTAAVPPVATNPPRPIRIGLALGGGAARGFAHIGVIKALEAQGIRPDIVTGTSAGSVVAALYASGMDGFTLNKLALTMDEATIADWALPFGTRFGGWLKGEALQNYVNRLVKNRPIESMKLPLGIVATDLKTGERILFRRGNTGQAVRASSSVPGVFQPVAIGGHDYVDGGLVEPVPVDAAREMGADFVIAVNISADPASQKNGGQSGVLLQTTAIMGQSINKMALARADVVIRPDLPDMGGSDFTSRNRAILAGEQATSAVLAALREKLAAARNRPATGGTVATQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LLH5</accession>
    <name>Q1LLH5_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">ABC-type transporter, periplasmic component</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Rmet_2122</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09001.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516835.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2122"/>
    <dbReference type="EnsemblBacteria" id="ABF09001">
        <property type="protein sequence ID" value="ABF09001"/>
        <property type="gene ID" value="Rmet_2122"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2122"/>
    <dbReference type="eggNOG" id="COG0715">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_052162_0_0_4"/>
    <dbReference type="OMA" id="PYTTFMA"/>
    <dbReference type="OrthoDB" id="785019at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001638">
        <property type="entry name" value="Solute-binding_3/MltF_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015168">
        <property type="entry name" value="SsuA/THI5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09084">
        <property type="entry name" value="NMT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00062">
        <property type="entry name" value="PBPb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193546" evidence="2">
        <location>
            <begin position="30"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="domain" description="PBPb" evidence="3">
        <location>
            <begin position="37"/>
            <end position="278"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00062"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF09001.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="347" mass="37317" checksum="215A0CE23199E792" modified="2006-05-30" version="1">MYNKKFWKQVLIALALFLAGWFTFGSAQAQGKPEKSKVTIAVGGKNLFYYLPLTIAERLGYFKEEGLDVEIVDFAGGAKALQAVVGGSADVVSGAYEHTINLQAKGQQYQEFVLQGRAPQIVLVVSNKTMPNFKSIADLKGKKIGVTAPGSSTNMMANFVLAKAGLKPSDVSFIGVGASAGAVAAMRSGQIDAMANLDPVISMLTQKNEVKIASDTRTIKETQTVFGGNMPSGCLYASVSFIQQNPNTTQAMANAMVRALKWLQKAGPSDIVKTVPESYLLGDRALYLAAWDKVKEAISPDGMMPTDGPHTALNTLQQFDPELKGKAIKLENTFTNNFVQKANAKYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LLJ4</accession>
    <name>Q1LLJ4_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Outer membrane protein assembly factor BamB</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">yfgL</name>
        <name evidence="6" type="synonym">bamB</name>
        <name evidence="8" type="ordered locus">Rmet_2103</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Part of the outer membrane protein assembly complex, which is involved in assembly and insertion of beta-barrel proteins into the outer membrane.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Part of the Bam complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cell outer membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the BamB family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08982.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516816.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2103"/>
    <dbReference type="EnsemblBacteria" id="ABF08982">
        <property type="protein sequence ID" value="ABF08982"/>
        <property type="gene ID" value="Rmet_2103"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2103"/>
    <dbReference type="eggNOG" id="COG1520">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027480_0_1_4"/>
    <dbReference type="OMA" id="GRVLWQR"/>
    <dbReference type="OrthoDB" id="1377603at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009279">
        <property type="term" value="C:cell outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043165">
        <property type="term" value="P:Gram-negative-bacterium-type cell outer membrane assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051205">
        <property type="term" value="P:protein insertion into membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00923">
        <property type="entry name" value="OM_assembly_BamB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017687">
        <property type="entry name" value="BamB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025666">
        <property type="entry name" value="PQQ-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018391">
        <property type="entry name" value="PQQ_beta_propeller_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002372">
        <property type="entry name" value="PQQ_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011047">
        <property type="entry name" value="Quinoprotein_ADH-like_supfam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13360">
        <property type="entry name" value="PQQ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13570">
        <property type="entry name" value="PQQ_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00564">
        <property type="entry name" value="PQQ"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50998">
        <property type="entry name" value="SSF50998"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03300">
        <property type="entry name" value="assembly_YfgL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0998">Cell outer membrane</keyword>
    <keyword evidence="5" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="2 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0564">Palmitate</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 6" id="KW-0732">Signal</keyword>
    <feature type="domain" description="PQQ_3" evidence="7">
        <location>
            <begin position="49"/>
            <end position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00923"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF13570"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ABF08982.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="383" mass="40358" checksum="92DE2D3B9BCFDB1B" modified="2006-05-30" version="1">MTSLLRVAATRTIVAGTCLGLLAGCSLFSKENKHPPTELKPIAATLSVKQAWKADVGKGGPYVMSPVASGNTVYVSSNGGTVLALDGATGQTRWKAKTDLDLTSGPGTDGTVTAVAGEKGAIYAFDTSGKQIWKKQVNGEVLSAPLVGNGLVIVRTTDTRLIGMDATTGERRWIYQRTQTPLNLRAAMGMVFAGDGIVTGFPGGKLGVLAPGNGALRWESTVSYPKGVSEIERLNDVTGVPAVNGRQVCATTFQGRVSCLELANGQPQWGKDFSSPSGLAQDDTSLYASDETSAVYAFDRQNGNERWKNTELRYRSLGAPVAVGRSVVVGDFEGFVHFLSREDGKILARMKTDGSAVTAAPVMAGQTLVVQTRDGDIYGFVPD</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-12-12" modified="2022-05-25" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LLJ6</accession>
    <name>HFQ_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RNA-binding protein Hfq</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">hfq</name>
        <name type="ordered locus">Rmet_2101</name>
    </gene>
    <organism>
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">RNA chaperone that binds small regulatory RNA (sRNAs) and mRNAs to facilitate mRNA translational regulation in response to envelope stress, environmental stress and changes in metabolite concentrations. Also binds with high specificity to tRNAs.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homohexamer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the Hfq family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08980.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516814.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q1LLJ6"/>
    <dbReference type="SMR" id="Q1LLJ6"/>
    <dbReference type="STRING" id="266264.Rmet_2101"/>
    <dbReference type="EnsemblBacteria" id="ABF08980">
        <property type="protein sequence ID" value="ABF08980"/>
        <property type="gene ID" value="Rmet_2101"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2101"/>
    <dbReference type="eggNOG" id="COG1923">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_113688_2_2_4"/>
    <dbReference type="OMA" id="QQMVYKH"/>
    <dbReference type="OrthoDB" id="1989988at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01716">
        <property type="entry name" value="Hfq"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00436">
        <property type="entry name" value="Hfq"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005001">
        <property type="entry name" value="Hfq"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010920">
        <property type="entry name" value="LSM_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34772">
        <property type="entry name" value="PTHR34772"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17209">
        <property type="entry name" value="Hfq"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50182">
        <property type="entry name" value="SSF50182"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02383">
        <property type="entry name" value="Hfq"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0346">Stress response</keyword>
    <feature type="chain" id="PRO_0000265178" description="RNA-binding protein Hfq">
        <location>
            <begin position="1"/>
            <end position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00436"/>
        </source>
    </evidence>
    <sequence length="79" mass="8904" checksum="94723A5F23353799" modified="2006-05-30" version="1">MSNKGQLLQDPFLNALRKEHVPVSIYLVNGIKLQGNIESFDQYVVLLRNTVTQMVYKHAISTVVPARAVNFRVDDSSES</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-01-15" modified="2022-05-25" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LLY7</accession>
    <name>HBOH_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">D-(-)-3-hydroxybutyrate oligomer hydrolase</fullName>
            <shortName evidence="1">3HB-oligomer hydrolase</shortName>
            <shortName evidence="1">3HBOH</shortName>
            <ecNumber evidence="1">3.1.1.22</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">Rmet_1960</name>
    </gene>
    <organism>
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Participates in the degradation of poly-3-hydroxybutyrate (PHB). It works downstream of poly(3-hydroxybutyrate) depolymerase, hydrolyzing D(-)-3-hydroxybutyrate oligomers of various length (3HB-oligomers) into 3HB-monomers.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(3R)-hydroxybutanoate dimer + H2O = 2 (R)-3-hydroxybutanoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10172"/>
            <dbReference type="ChEBI" id="CHEBI:10979"/>
            <dbReference type="ChEBI" id="CHEBI:10983"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="EC" id="3.1.1.22"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Lipid metabolism; butanoate metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the D-(-)-3-hydroxybutyrate oligomer hydrolase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.22" evidence="1"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08839.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516685.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q1LLY7"/>
    <dbReference type="STRING" id="266264.Rmet_1960"/>
    <dbReference type="EnsemblBacteria" id="ABF08839">
        <property type="protein sequence ID" value="ABF08839"/>
        <property type="gene ID" value="Rmet_1960"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1960"/>
    <dbReference type="eggNOG" id="ENOG502Z8QU">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_420258_0_0_4"/>
    <dbReference type="OMA" id="NPEKDWG"/>
    <dbReference type="OrthoDB" id="865015at2"/>
    <dbReference type="UniPathway" id="UPA00863"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047989">
        <property type="term" value="F:hydroxybutyrate-dimer hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019605">
        <property type="term" value="P:butyrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01906">
        <property type="entry name" value="3HBOH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016582">
        <property type="entry name" value="OHBut_olig_hydro_put"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10605">
        <property type="entry name" value="3HBOH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF011409">
        <property type="entry name" value="HObutyrate_olig_hydrol"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0964">Secreted</keyword>
    <keyword id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5000118750" description="D-(-)-3-hydroxybutyrate oligomer hydrolase">
        <location>
            <begin position="37"/>
            <end position="722"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="671"/>
            <end position="697"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="1">
        <location>
            <position position="327"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="722" mass="73917" checksum="911C2C8140F5A68D" modified="2006-05-30" version="1" precursor="true"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-10-07" version="61" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LM26</accession>
    <name>Q1LM26_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">UBP-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_1921</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08800.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1921"/>
    <dbReference type="EnsemblBacteria" id="ABF08800">
        <property type="protein sequence ID" value="ABF08800"/>
        <property type="gene ID" value="Rmet_1921"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1921"/>
    <dbReference type="eggNOG" id="COG5207">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_163943_1_0_4"/>
    <dbReference type="OMA" id="WGWCYVD"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001607">
        <property type="entry name" value="Znf_UBP"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02148">
        <property type="entry name" value="zf-UBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50271">
        <property type="entry name" value="ZF_UBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UBP-type" evidence="1">
        <location>
            <begin position="18"/>
            <end position="86"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF08800.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="100" mass="11228" checksum="0A5CCB2C09219226" modified="2006-05-30" version="1">MKTCDHADTIRDVVPSARGCEECLNVGSTWVHLRLCRACGHVGCCDNSPLRHARAHYEKTGHPIIEGYDPPEGWGWCYVDREEVTLPDQTPQLGPIPRFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="60" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LM28</accession>
    <name>Q1LM28_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_1919</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08798.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516651.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1919"/>
    <dbReference type="EnsemblBacteria" id="ABF08798">
        <property type="protein sequence ID" value="ABF08798"/>
        <property type="gene ID" value="Rmet_1919"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1919"/>
    <dbReference type="eggNOG" id="COG3619">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_090580_0_0_4"/>
    <dbReference type="OMA" id="FTNHVTG"/>
    <dbReference type="OrthoDB" id="1344403at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010699">
        <property type="entry name" value="DUF1275"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06912">
        <property type="entry name" value="DUF1275"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="24"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="68"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="101"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="126"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="187"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="212"/>
            <end position="232"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF08798.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="237" mass="25126" checksum="ED6CEF2AAE54BC1F" modified="2006-05-30" version="1">MTAISDSQSHDNPAASLWAQCRAAVLLTIVGGAIDTIGFIALLGFFTAHVTGNLVLAGAALVKGGAGLWVKLGAIPLFIVTVMLAKMWIDRCNQSHKTLGWLFVAEAVFLSAFMLVGLYFDPLLDPGAGELALTGGLGLMALAIRNTASKTLIKHISPSTMMTGNTTQFGIDLSNFVRTFSREHGVAVLKSGSIVVGFVAGAFLGAVLYMRIGFWSVLPFTLAILYLASLAFRKQFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LM65</accession>
    <name>Q1LM65_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Peptidyl-prolyl cis-trans isomerase</fullName>
            <ecNumber evidence="4">5.2.1.8</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">ppiD</name>
        <name evidence="4" type="ordered locus">Rmet_1882</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EC" id="5.2.1.8" evidence="4"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08761.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516608.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1882"/>
    <dbReference type="EnsemblBacteria" id="ABF08761">
        <property type="protein sequence ID" value="ABF08761"/>
        <property type="gene ID" value="Rmet_1882"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1882"/>
    <dbReference type="eggNOG" id="COG0760">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023843_1_2_4"/>
    <dbReference type="OMA" id="DNSQGWI"/>
    <dbReference type="OrthoDB" id="1201303at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003755">
        <property type="term" value="F:peptidyl-prolyl cis-trans isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.50.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046357">
        <property type="entry name" value="PPIase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000297">
        <property type="entry name" value="PPIase_PpiC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023058">
        <property type="entry name" value="PPIase_PpiC_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027304">
        <property type="entry name" value="Trigger_fact/SurA_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109998">
        <property type="entry name" value="SSF109998"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01096">
        <property type="entry name" value="PPIC_PPIASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50198">
        <property type="entry name" value="PPIC_PPIASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2 4" id="KW-0413">Isomerase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0697">Rotamase</keyword>
    <feature type="domain" description="PpiC" evidence="3">
        <location>
            <begin position="269"/>
            <end position="372"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF08761.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="647" mass="70518" checksum="7E878D0C73D32DA4" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LMK7</accession>
    <name>Q1LMK7_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Putative membrane protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Rmet_1740</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08619.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516471.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1740"/>
    <dbReference type="EnsemblBacteria" id="ABF08619">
        <property type="protein sequence ID" value="ABF08619"/>
        <property type="gene ID" value="Rmet_1740"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1740"/>
    <dbReference type="eggNOG" id="COG1566">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033219_0_0_4"/>
    <dbReference type="OMA" id="APEHRHG"/>
    <dbReference type="OrthoDB" id="1832217at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="46"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="224"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF08619.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="443" mass="48359" checksum="64A5144D2BDD38D0" modified="2006-05-30" version="1">MDKKVDQKDSVPARVKPSQHPPHHDSLMQRRAGVPGLPLWRVVPRLASYGMVVFIIWVVLSVMFPYIFTRSSERAIVNSPVTLVTTPVEGVVTKQVVTVGGSFKAGDPLMALQNPNMDRALLVELTGKQLDNQKRLEAAKVKLEADQTRMASTGGDIKRYQASADREHASRVRGIEARLAVAKQQIDAQEDVVNRNQAMQWAGAVSQAYTDASRNQLTVLAGSRDAIQAELDSARNSSEAARSKVFSSSADGALGALSQKQDELKAEITQTQAEITQLEQYGAEVDKLVTAEQERLERVSNLDIKAYASGIVEDVLTPPGTRVAAGATLMRTTNCSQPNVVAVFPRSLSKDLLPGAKLTVLVDGVPTPQPGTVAEIMPRAPEGDQARYFVPFPPIEKNEIYLIAKLNKPLPDLRNRGSGSTDRCALGHWAKVSLDRPWFTRLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="102" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LMR1</accession>
    <name>Q1LMR1_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Fused ribosome-associated ATPase: ATP-binding protein ATP-binding protein membrane protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">rbbA</name>
        <name evidence="14" type="ordered locus">Rmet_1684</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08565.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516413.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1684"/>
    <dbReference type="EnsemblBacteria" id="ABF08565">
        <property type="protein sequence ID" value="ABF08565"/>
        <property type="gene ID" value="Rmet_1684"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1684"/>
    <dbReference type="eggNOG" id="COG0842">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG1129">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_16_3_4"/>
    <dbReference type="OMA" id="FTENFTD"/>
    <dbReference type="OrthoDB" id="870493at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043190">
        <property type="term" value="C:ATP-binding cassette (ABC) transporter complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000412">
        <property type="entry name" value="ABC_2_transport"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51012">
        <property type="entry name" value="ABC_TM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 10 14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 11" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9 11 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-1278">Translocase</keyword>
    <keyword evidence="4 11 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="570"/>
            <end position="588"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="722"/>
            <end position="747"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="776"/>
            <end position="797"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="804"/>
            <end position="826"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="832"/>
            <end position="853"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="865"/>
            <end position="883"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="895"/>
            <end position="915"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="13">
        <location>
            <begin position="12"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="13">
        <location>
            <begin position="277"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="44"/>
            <end position="51"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="309"/>
            <end position="316"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00442"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ABF08565.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="919" mass="100250" checksum="FF1A2952D6C55B5C" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="70" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LN23</accession>
    <name>Q1LN23_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_1570</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08453.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516307.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1570"/>
    <dbReference type="EnsemblBacteria" id="ABF08453">
        <property type="protein sequence ID" value="ABF08453"/>
        <property type="gene ID" value="Rmet_1570"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1570"/>
    <dbReference type="eggNOG" id="COG3812">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_090929_1_0_4"/>
    <dbReference type="OMA" id="DMFPLVR"/>
    <dbReference type="OrthoDB" id="2020712at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.450">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034660">
        <property type="entry name" value="DinB/YfiT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018531">
        <property type="entry name" value="DUF1993"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36922">
        <property type="entry name" value="PTHR36922"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09351">
        <property type="entry name" value="DUF1993"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109854">
        <property type="entry name" value="SSF109854"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF08453.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="170" mass="18663" checksum="81B5C75BE6FBDFDF" modified="2006-05-30" version="1">MALSMYDVSVPVFIRALTNLSAILEKGAAHAKAQGMDAAELIQTRLIADMDPLPAQVQRASDAAKGCAARLAGLETPSFPDTEATFPELQERIAKTIGYLKTIRPEQFEGSETRTVELKLRHGPITFDGKNYLLGFALPNFYFHVTTAYDILRHKGVQIGKMDFLGAPPR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-10-03" modified="2022-08-03" version="96" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LN92</accession>
    <name>RBL_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Ribulose bisphosphate carboxylase large chain</fullName>
            <shortName evidence="1">RuBisCO large subunit</shortName>
            <ecNumber evidence="1">4.1.1.39</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">cbbL</name>
        <name type="ordered locus">Rmet_1501</name>
    </gene>
    <organism>
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">RuBisCO catalyzes two reactions: the carboxylation of D-ribulose 1,5-bisphosphate, the primary event in carbon dioxide fixation, as well as the oxidative fragmentation of the pentose substrate. Both reactions occur simultaneously and in competition at the same active site.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>2 (2R)-3-phosphoglycerate + 2 H(+) = CO2 + D-ribulose 1,5-bisphosphate + H2O</text>
            <dbReference type="Rhea" id="RHEA:23124"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57870"/>
            <dbReference type="ChEBI" id="CHEBI:58272"/>
            <dbReference type="EC" id="4.1.1.39"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>D-ribulose 1,5-bisphosphate + O2 = (2R)-3-phosphoglycerate + 2-phosphoglycolate + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:36631"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:57870"/>
            <dbReference type="ChEBI" id="CHEBI:58033"/>
            <dbReference type="ChEBI" id="CHEBI:58272"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="1">Binds 1 Mg(2+) ion per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Heterohexadecamer of 8 large chains and 8 small chains.</text>
    </comment>
    <comment type="miscellaneous">
        <text evidence="1">The basic functional RuBisCO is composed of a large chain homodimer in a 'head-to-tail' conformation. In form I RuBisCO this homodimer is arranged in a barrel-like tetramer with the small subunits forming a tetrameric 'cap' on each end of the 'barrel'.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the RuBisCO large chain family. Type I subfamily.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.39" evidence="1"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08384.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516247.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q1LN92"/>
    <dbReference type="SMR" id="Q1LN92"/>
    <dbReference type="STRING" id="266264.Rmet_1501"/>
    <dbReference type="EnsemblBacteria" id="ABF08384">
        <property type="protein sequence ID" value="ABF08384"/>
        <property type="gene ID" value="Rmet_1501"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1501"/>
    <dbReference type="eggNOG" id="COG1850">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031450_2_0_4"/>
    <dbReference type="OMA" id="EYRETYW"/>
    <dbReference type="OrthoDB" id="848380at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016984">
        <property type="term" value="F:ribulose-bisphosphate carboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019253">
        <property type="term" value="P:reductive pentose-phosphate cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01338">
        <property type="entry name" value="RuBisCO_L_type1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033966">
        <property type="entry name" value="RuBisCO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020878">
        <property type="entry name" value="RuBisCo_large_chain_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000685">
        <property type="entry name" value="RuBisCO_lsu_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036376">
        <property type="entry name" value="RuBisCO_lsu_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017443">
        <property type="entry name" value="RuBisCO_lsu_fd_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036422">
        <property type="entry name" value="RuBisCO_lsu_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020888">
        <property type="entry name" value="RuBisCO_lsuI"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42704">
        <property type="entry name" value="PTHR42704"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00016">
        <property type="entry name" value="RuBisCO_large"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02788">
        <property type="entry name" value="RuBisCO_large_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00014">
        <property type="entry name" value="RuBisCO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51649">
        <property type="entry name" value="SSF51649"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54966">
        <property type="entry name" value="SSF54966"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00157">
        <property type="entry name" value="RUBISCO_LARGE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0113">Calvin cycle</keyword>
    <keyword id="KW-0120">Carbon dioxide fixation</keyword>
    <keyword id="KW-0456">Lyase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0503">Monooxygenase</keyword>
    <keyword id="KW-0560">Oxidoreductase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000251455" description="Ribulose bisphosphate carboxylase large chain">
        <location>
            <begin position="1"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="1">
        <location>
            <position position="168"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="1">
        <location>
            <position position="287"/>
        </location>
    </feature>
    <feature type="binding site" description="in homodimeric partner" evidence="1">
        <location>
            <position position="116"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="166"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="170"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" description="via carbamate group" evidence="1">
        <location>
            <position position="194"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="196"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="197"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="288"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="320"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="372"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="1">
        <location>
            <position position="327"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-carboxylysine" evidence="1">
        <location>
            <position position="194"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01338"/>
        </source>
    </evidence>
    <sequence length="473" mass="52608" checksum="6CBA7F7722693534" modified="2006-05-30" version="1">MAAKTYNAGVKEYRQTYWTPEYTPKDTDLLACFKVTPQPGVAREEVAAAVAAESSTGTWTTVWTDLLTDLDYYKGRAYRIEDVPGDDTCFYAFVAYPIDLFEEGSIVNVLTSLVGNVFGFKALRALRLEDVRFPIAYVMTCGGPPHGIQVERDIMNKYGRPLLGCTIKPKLGLSAKNYGRAVYECLRGGLDFTKDDENVNSQPFMRWKQRFDFVQEATEKAQRETGERKGHYLNVTAPTPEEMYKRAEYAKEIGAPIIMHDYLTGGFCANTGLANWCRDNGMLLHIHRAMHAVLDRNPHHGIHFRVLTKCLRLSGGDHLHSGTVVGKLEGDRDATLGWIDIMRDDFIKEDRSRGIMFDQDFGSMPGVMPVASGGIHVWHMPALVTIFGDDSVLQFGGGTLGHPWGNAAGAAANRVALEACVEARNKGVAVEKEGKSILTEAATHSPELKIAMETWKEIKFEFDTVDKLDVAHK</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="58" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LNB1</accession>
    <name>Q1LNB1_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Lipoprotein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_1482</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08365.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516231.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1482"/>
    <dbReference type="EnsemblBacteria" id="ABF08365">
        <property type="protein sequence ID" value="ABF08365"/>
        <property type="gene ID" value="Rmet_1482"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1482"/>
    <dbReference type="eggNOG" id="COG3616">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_119528_1_1_4"/>
    <dbReference type="OMA" id="HLTWLLA"/>
    <dbReference type="OrthoDB" id="1558145at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021341">
        <property type="entry name" value="DUF2958"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11171">
        <property type="entry name" value="DUF2958"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF08365.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="115" mass="12640" checksum="AAF57B869E3144C5" modified="2006-05-30" version="1">MNRLITEDERRQLLVHGQARAGGEAIDPLPVARLFTPDAHLIWLLAALDPADGDTAWGLIDLGLGMPELGTVKLSDLASIVGPRQQPVMRDRYFQAARPLSEYLRLAQENGSILD</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="63" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LNC1</accession>
    <name>Q1LNC1_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KAP P-loop</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_1472</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08355.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516220.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1472"/>
    <dbReference type="EnsemblBacteria" id="ABF08355">
        <property type="protein sequence ID" value="ABF08355"/>
        <property type="gene ID" value="Rmet_1472"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1472"/>
    <dbReference type="eggNOG" id="COG4928">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028167_1_0_4"/>
    <dbReference type="OrthoDB" id="1668340at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011646">
        <property type="entry name" value="KAP_P-loop"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07693">
        <property type="entry name" value="KAP_NTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="KAP NTPase" evidence="1">
        <location>
            <begin position="18"/>
            <end position="381"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF07693"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF08355.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="644" mass="69870" checksum="3DAC30EF3B0C3203" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="63" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LNE2</accession>
    <name>Q1LNE2_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Rmet_1451</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08334.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF08334">
        <property type="protein sequence ID" value="ABF08334"/>
        <property type="gene ID" value="Rmet_1451"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1451"/>
    <dbReference type="HOGENOM" id="CLU_1502284_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="36"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="95"/>
            <end position="158"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF08334.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="179" mass="19874" checksum="D4E8B9C2B1A15709" modified="2006-05-30" version="1">MFFMTADRQPLPPNRAAGRKAPNDLAYSGSSHALKAAGLVFSGVALSLAGIFAIIEYSSSHSRALIAADINTVSAEVRAINTPLDSQQAATDQRFNRVEQRLDKFDERFERIDERFESLESKFDLKLEHLETRFDSKLERLETKLDQKLDTVVELLLQLVSQKAEQPAGTGQPAAGRAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="59" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LNK7</accession>
    <name>Q1LNK7_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_1386</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08269.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1386"/>
    <dbReference type="EnsemblBacteria" id="ABF08269">
        <property type="protein sequence ID" value="ABF08269"/>
        <property type="gene ID" value="Rmet_1386"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1386"/>
    <dbReference type="eggNOG" id="COG3459">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021468_0_0_4"/>
    <dbReference type="OrthoDB" id="1384939at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021478">
        <property type="entry name" value="DUF3131"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11329">
        <property type="entry name" value="DUF3131"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="40"/>
            <end position="94"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF08269.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="529" mass="57074" checksum="24FCD191D05945D3" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LNV7</accession>
    <name>Q1LNV7_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">HypF1 hydrogenase maturation protein (CMGI-2)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">hypF1</name>
        <name evidence="3" type="ordered locus">Rmet_1284</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the carbamoyltransferase HypF family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08169.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516051.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1284"/>
    <dbReference type="EnsemblBacteria" id="ABF08169">
        <property type="protein sequence ID" value="ABF08169"/>
        <property type="gene ID" value="Rmet_1284"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1284"/>
    <dbReference type="eggNOG" id="COG0068">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009164_1_0_4"/>
    <dbReference type="OMA" id="GGCFFNR"/>
    <dbReference type="OrthoDB" id="1428450at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041440">
        <property type="entry name" value="HypF_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17788">
        <property type="entry name" value="HypF_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HypF_C" evidence="2">
        <location>
            <begin position="3"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF17788"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF08169.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="379" mass="39347" checksum="1160304066B369BF" modified="2006-05-30" version="1">MSATVLAVGAWLKNAACLVLDGRAHWSPVHGDLSDPAACDALERSVQALARQAEAAGAPVQAIAHDLHPDFFSTQLAIATADALGVPAIGVQHHHAHLAAVIAEHAIDGPVVGLALDGVGLGTDGTPWGGELLWLEGAQWQRLGHLQPLALPGGDRAAREPWRMAASALHALDRADEIVPRFAPVVGEAVAAGVRQMLDKRLNCPLTSSAGRWFDAAAGALGLCHHQQDEAQAAIALEQAATRAISALPDLPPLAGAVRHDDGRIDLRAVLAQLFDAPDADTGAAGFHLALADALAEALITAARGRGARTAALGGGCFFNRILRERTVQRLQAAGLQVFQTGDRGCGDAGLAIGQAWVAAQQLQTPRQEPHVEETVPCA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LNW0</accession>
    <name>Q1LNW0_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Carbamoyl phosphate phosphatase, hydrogenase 3 maturation protein (CMGI-2)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">hypE1</name>
        <name evidence="4" type="ordered locus">Rmet_1281</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the HypE family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08166.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516048.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1281"/>
    <dbReference type="EnsemblBacteria" id="ABF08166">
        <property type="protein sequence ID" value="ABF08166"/>
        <property type="gene ID" value="Rmet_1281"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1281"/>
    <dbReference type="eggNOG" id="COG0309">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_049733_0_0_4"/>
    <dbReference type="OMA" id="TINDLAC"/>
    <dbReference type="OrthoDB" id="1016556at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd02197">
        <property type="entry name" value="HypE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.650.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011854">
        <property type="entry name" value="HypE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010918">
        <property type="entry name" value="PurM-like_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036676">
        <property type="entry name" value="PurM-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016188">
        <property type="entry name" value="PurM-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036921">
        <property type="entry name" value="PurM-like_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30303">
        <property type="entry name" value="PTHR30303"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00586">
        <property type="entry name" value="AIRS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02769">
        <property type="entry name" value="AIRS_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005644">
        <property type="entry name" value="Hdrgns_mtr_HypE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55326">
        <property type="entry name" value="SSF55326"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56042">
        <property type="entry name" value="SSF56042"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02124">
        <property type="entry name" value="hypE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AIRS" evidence="2">
        <location>
            <begin position="66"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="domain" description="AIRS_C" evidence="3">
        <location>
            <begin position="195"/>
            <end position="347"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006243"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF02769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF08166.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="370" mass="38460" checksum="6A9FBC9B20ADF85F" modified="2006-05-30" version="1">MRTEHHQAGAGEAPAPKPVKKNYIRPLDIKHGRIDMGHGAGGKAAAQLIEELFLAAFDNEFLRQGDDGAVLALPAFDPAEGRLVMATDGHVISPLFFPGGDIGCLSVHGTVNDVAMSGARPLYLSASFIIEEGFPLVDLQRIVQSMAAAAREAGVPVVTGDTKVVEQGKGDGVFISTTGVGVLPPGVSISGRNARPGDVVLVSGSIGDHGVAVLSQRESLAFETTIESDTAALHTLVAAMLAAAPGAVRVLRDPTRGGLATTLNEVARQSGVGMLLQEAAIPVKPQVDAACELLGLDPLYVANEGKLIAVVAPEAAEAVLAAMRAHPLGQDAARIGEVTTDPHHFVQMATRFGGRRVVDWLSGEQLPRIC</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="69" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LNW4</accession>
    <name>Q1LNW4_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Putative transporter fused subunits of ABC superfamily: ATP-binding components</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_1277</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08162.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1277"/>
    <dbReference type="EnsemblBacteria" id="ABF08162">
        <property type="protein sequence ID" value="ABF08162"/>
        <property type="gene ID" value="Rmet_1277"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1277"/>
    <dbReference type="eggNOG" id="COG1131">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2540125_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF08162.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="83" mass="9453" checksum="61A0AB3A435FED76" modified="2006-05-30" version="1">MIHRTRSLFLDEPTSGVDPITRREFWTHINGLARKGVTIMVTTHFMDEAEYCDRVAMLSRAQLIALDTPDALSMSSINRILFA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="70" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LP06</accession>
    <name>Q1LP06_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_1234</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08120.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1234"/>
    <dbReference type="EnsemblBacteria" id="ABF08120">
        <property type="protein sequence ID" value="ABF08120"/>
        <property type="gene ID" value="Rmet_1234"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1234"/>
    <dbReference type="eggNOG" id="COG5570">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_175516_1_1_4"/>
    <dbReference type="OMA" id="DEIAWCE"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.280.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007420">
        <property type="entry name" value="DUF465"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038444">
        <property type="entry name" value="DUF465_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04325">
        <property type="entry name" value="DUF465"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="9"/>
            <end position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF08120.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="78" mass="9171" checksum="533548D86AC51D09" modified="2006-05-30" version="1">MAARQGRGRRNMDENLNTLARQIIELQIEHRDLDFMIDRLASDPSHDELQLRRLKKRRLKLKDAITLLQVQVEPDVPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="95" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LP94</accession>
    <name>Q1LP94_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Electron transfer flavoprotein-ubiquinone oxidoreductase</fullName>
            <shortName evidence="10">ETF-QO</shortName>
            <ecNumber evidence="10">1.5.5.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">etfD</name>
        <name evidence="12" type="ordered locus">Rmet_1146</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2 10">Accepts electrons from ETF and reduces ubiquinone.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>a ubiquinone + reduced [electron-transfer flavoprotein] = a ubiquinol + H(+) + oxidized [electron-transfer flavoprotein]</text>
            <dbReference type="Rhea" id="RHEA:24052"/>
            <dbReference type="Rhea" id="RHEA-COMP:9565"/>
            <dbReference type="Rhea" id="RHEA-COMP:9566"/>
            <dbReference type="Rhea" id="RHEA-COMP:10685"/>
            <dbReference type="Rhea" id="RHEA-COMP:10686"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16389"/>
            <dbReference type="ChEBI" id="CHEBI:17976"/>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
            <dbReference type="ChEBI" id="CHEBI:58307"/>
            <dbReference type="EC" id="1.5.5.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 10">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="10">Binds 1 [4Fe-4S] cluster.</text>
    </comment>
    <dbReference type="EC" id="1.5.5.1" evidence="10"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08032.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515934.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1146"/>
    <dbReference type="EnsemblBacteria" id="ABF08032">
        <property type="protein sequence ID" value="ABF08032"/>
        <property type="gene ID" value="Rmet_1146"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1146"/>
    <dbReference type="eggNOG" id="COG0644">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG2440">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009667_4_1_4"/>
    <dbReference type="OMA" id="GGGWMYH"/>
    <dbReference type="OrthoDB" id="1770293at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004174">
        <property type="term" value="F:electron-transferring-flavoprotein dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040156">
        <property type="entry name" value="ETF-QO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10617">
        <property type="entry name" value="PTHR10617"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="SSF51905"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 10" id="KW-0249">Electron transport</keyword>
    <keyword evidence="5 10" id="KW-0274">FAD</keyword>
    <keyword evidence="4 10" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="10" id="KW-0408">Iron</keyword>
    <keyword evidence="8 10" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 10 12" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 10" id="KW-0813">Transport</keyword>
    <keyword evidence="9 10 12" id="KW-0830">Ubiquinone</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="11">
        <location>
            <begin position="521"/>
            <end position="550"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU366068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ABF08032.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="561" mass="61512" checksum="367D397240390EB6" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LPN5</accession>
    <name>Q1LPN5_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Thioredoxin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_1005</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07891.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515805.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1005"/>
    <dbReference type="EnsemblBacteria" id="ABF07891">
        <property type="protein sequence ID" value="ABF07891"/>
        <property type="gene ID" value="Rmet_1005"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1005"/>
    <dbReference type="eggNOG" id="COG0526">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_137795_0_0_4"/>
    <dbReference type="OMA" id="FNCEMAK"/>
    <dbReference type="OrthoDB" id="1630944at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00085">
        <property type="entry name" value="Thioredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="SSF52833"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thioredoxin" evidence="1">
        <location>
            <begin position="20"/>
            <end position="115"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF07891.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="134" mass="14707" checksum="55BD6B420DFCEEC4" modified="2006-05-30" version="1">MSEENQPHLDHRAFEALDMAEVDGENIDAAIANAGDDLVCVFFWGVDCFNCEMAKKAILANPEPIRALDLRWLHANVYAHPELGRRFGLHGIPVFMFFHKGKKLGRATGWHGHGQFSAAVGNARLKASGKPLAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LPP9</accession>
    <name>Q1LPP9_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Peptidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">tldD</name>
        <name evidence="6" type="ordered locus">Rmet_0991</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase U62 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07877.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008641746.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0991"/>
    <dbReference type="EnsemblBacteria" id="ABF07877">
        <property type="protein sequence ID" value="ABF07877"/>
        <property type="gene ID" value="Rmet_0991"/>
    </dbReference>
    <dbReference type="GeneID" id="60821852"/>
    <dbReference type="KEGG" id="rme:Rmet_0991"/>
    <dbReference type="eggNOG" id="COG0312">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026425_1_0_4"/>
    <dbReference type="OMA" id="TDFWGSM"/>
    <dbReference type="OrthoDB" id="806701at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008237">
        <property type="term" value="F:metallopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2290.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045569">
        <property type="entry name" value="Metalloprtase-TldD/E_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045570">
        <property type="entry name" value="Metalloprtase-TldD/E_cen_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002510">
        <property type="entry name" value="Metalloprtase-TldD/E_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025502">
        <property type="entry name" value="TldD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035068">
        <property type="entry name" value="TldD/PmbA_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036059">
        <property type="entry name" value="TldD/PmbA_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01523">
        <property type="entry name" value="PmbA_TldD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19289">
        <property type="entry name" value="PmbA_TldD_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19290">
        <property type="entry name" value="PmbA_TldD_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004919">
        <property type="entry name" value="TldD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111283">
        <property type="entry name" value="SSF111283"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PmbA_TldD" evidence="3">
        <location>
            <begin position="44"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="domain" description="PmbA_TldD_M" evidence="5">
        <location>
            <begin position="135"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="domain" description="PmbA_TldD_C" evidence="4">
        <location>
            <begin position="251"/>
            <end position="484"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005836"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01523"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF19289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF19290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABF07877.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="486" mass="51387" checksum="D6E4617B2E9ABE6D" modified="2006-05-30" version="1">MNAGDLGIRNLATAQQLLLAPYGLDEAKLQRVLADIFTHKVDYADLYFQHTRNEAWSLEEGIVKSGSFSIDQGVGVRAVSGDKTAFAYSDDISLPALSQAAAATRTIGKSGGGRVKVAGSLASHTGRNLYAPNDPLDSMTATEKVALLEKIEKLARAKDPRVVQVMAGLAGEYDVVLVARSDGVIAADVRPLVRVSVTVIAEQNGRREIGSSGGGGRYAYGYFTDDLLRKYVDEAVSSALVNLEARPAPAGAMTVVLGPGWPGVLLHEAVGHGLEGDFNRKGSSAFAGRMGERVAAKGVTVVDDGTIANRRGSLNLDDEGNPTQCTTLIEDGILRGYIQDTLNARLMKMPVTGNARRESYAALPMPRMTNTYMLNGDRDPQEILASVKRGLYAVNFGGGQVDITNGKFVFSASEAYMIEDGKITYPVKGATLVGNGPESLKDVTMIGNDMRLDSGVGVCGKEGQSVPVGVGQPTLRIENMTVGGTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LPQ1</accession>
    <name>Q1LPQ1_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Phospho-2-dehydro-3-deoxyheptonate aldolase</fullName>
            <ecNumber evidence="5">2.5.1.54</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">aroG</name>
        <name evidence="7" type="ordered locus">Rmet_0989</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2 5">Stereospecific condensation of phosphoenolpyruvate (PEP) and D-erythrose-4-phosphate (E4P) giving rise to 3-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 5">
            <text>D-erythrose 4-phosphate + H2O + phosphoenolpyruvate = 7-phospho-2-dehydro-3-deoxy-D-arabino-heptonate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:14717"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16897"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58394"/>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
            <dbReference type="EC" id="2.5.1.54"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3 5">Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 1/7.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 5">Belongs to the class-I DAHP synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.54" evidence="5"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07875.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515790.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0989"/>
    <dbReference type="EnsemblBacteria" id="ABF07875">
        <property type="protein sequence ID" value="ABF07875"/>
        <property type="gene ID" value="Rmet_0989"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0989"/>
    <dbReference type="eggNOG" id="COG0722">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030903_0_1_4"/>
    <dbReference type="OMA" id="DINTGLR"/>
    <dbReference type="UniPathway" id="UPA00053">
        <property type="reaction ID" value="UER00084"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003849">
        <property type="term" value="F:3-deoxy-7-phosphoheptulonate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009073">
        <property type="term" value="P:aromatic amino acid family biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008652">
        <property type="term" value="P:cellular amino acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009423">
        <property type="term" value="P:chorismate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006218">
        <property type="entry name" value="DAHP1/KDSA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006219">
        <property type="entry name" value="DHAP_synth_1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21225">
        <property type="entry name" value="PTHR21225"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00793">
        <property type="entry name" value="DAHP_synth_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001361">
        <property type="entry name" value="DAHP_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00034">
        <property type="entry name" value="aroFGH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="5" id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="DAHP_synth_1" evidence="6">
        <location>
            <begin position="43"/>
            <end position="341"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00003726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007985"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRNR" id="PIRNR001361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABF07875.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="359" mass="38936" checksum="25F19FF1CA12B79B" modified="2006-05-30" version="1">MLKNTDDLRIRELKELLPPAHLIREFACSEAASDVIYGARQAMHRILHGMDDRLIVIIGPCSIHDTRAALEYAKLLKVQRDRFAGELEIVMRVYFEKPRTTVGWKGLINDPHMDGSFKINDGLRTARELLLNISEMGVPTGTEYLDMISPQYIADLVSWGAIGARTTESQVHRELASGLSCPVGFKNGTDGNVKIAVDAIKAASQPHHFLSVTKGGHSAIVSTSGNEDCHIILRGGKTPNYDAASVQEACDAISKSGLAARLMIDASHANSSKKHENQIPVCEDIGKQIAGGEQRIVGVMVESHLVAGRQDHVQGTPVENLTYGQSVTDACIAWDDSVAVLETLANAVKQRRLVTGSGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LPU4</accession>
    <name>Q1LPU4_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative acyl dehydratase with a MaoC-like domain</fullName>
            <ecNumber evidence="3">4.2.1.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_0946</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EC" id="4.2.1.-" evidence="3"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07832.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515757.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0946"/>
    <dbReference type="EnsemblBacteria" id="ABF07832">
        <property type="protein sequence ID" value="ABF07832"/>
        <property type="gene ID" value="Rmet_0946"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0946"/>
    <dbReference type="eggNOG" id="COG2030">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_094876_1_0_4"/>
    <dbReference type="OMA" id="ISSGWMT"/>
    <dbReference type="OrthoDB" id="1563802at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029069">
        <property type="entry name" value="HotDog_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002539">
        <property type="entry name" value="MaoC-like_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01575">
        <property type="entry name" value="MaoC_dehydratas"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54637">
        <property type="entry name" value="SSF54637"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0456">Lyase</keyword>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="50"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="domain" description="MaoC-like" evidence="2">
        <location>
            <begin position="19"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01575"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF07832.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="149" mass="16504" checksum="FE4268E5741172F7" modified="2006-05-30" version="1">MSKMYFEDYVVGSEHPLGSYTVTEEEIISFARQYDPQPFHVDKAAAEQSIYGGLISSGWMTCGIIMRLLVQSMGDRSTSMGSPGVDEVRWIKPVYAGDTLTAVLRILEARPSSSKPDRGVVMTQWVATNQKGEVVCTVKGMGMYKRRPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="109" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LPW5</accession>
    <name>Q1LPW5_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 4">Triosephosphate isomerase</fullName>
            <shortName evidence="3">TIM</shortName>
            <shortName evidence="3">TPI</shortName>
            <ecNumber evidence="3 4">5.3.1.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">Triose-phosphate isomerase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">cbbJ2</name>
        <name evidence="3" type="synonym">tpiA</name>
        <name evidence="6" type="ordered locus">Rmet_0925</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Involved in the gluconeogenesis. Catalyzes stereospecifically the conversion of dihydroxyacetone phosphate (DHAP) to D-glyceraldehyde-3-phosphate (G3P).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3 4">
            <text>D-glyceraldehyde 3-phosphate = dihydroxyacetone phosphate</text>
            <dbReference type="Rhea" id="RHEA:18585"/>
            <dbReference type="ChEBI" id="CHEBI:57642"/>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
            <dbReference type="EC" id="5.3.1.1"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3 4">Carbohydrate biosynthesis; gluconeogenesis.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3 4">Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate from glycerone phosphate: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3 4">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3 4">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 4">Belongs to the triosephosphate isomerase family.</text>
    </comment>
    <dbReference type="EC" id="5.3.1.1" evidence="3 4"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07811.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0925"/>
    <dbReference type="EnsemblBacteria" id="ABF07811">
        <property type="protein sequence ID" value="ABF07811"/>
        <property type="gene ID" value="Rmet_0925"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0925"/>
    <dbReference type="eggNOG" id="COG0149">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024251_2_3_4"/>
    <dbReference type="OMA" id="HTEVICA"/>
    <dbReference type="UniPathway" id="UPA00109">
        <property type="reaction ID" value="UER00189"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00138"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004807">
        <property type="term" value="F:triose-phosphate isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006094">
        <property type="term" value="P:gluconeogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006096">
        <property type="term" value="P:glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00311">
        <property type="entry name" value="TIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00147_B">
        <property type="entry name" value="TIM_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035990">
        <property type="entry name" value="TIM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022896">
        <property type="entry name" value="TrioseP_Isoase_bac/euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000652">
        <property type="entry name" value="Triosephosphate_isomerase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020861">
        <property type="entry name" value="Triosephosphate_isomerase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21139">
        <property type="entry name" value="PTHR21139"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00121">
        <property type="entry name" value="TIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51351">
        <property type="entry name" value="SSF51351"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00419">
        <property type="entry name" value="tim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00171">
        <property type="entry name" value="TIM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51440">
        <property type="entry name" value="TIM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="1 3 4" id="KW-0312">Gluconeogenesis</keyword>
    <keyword evidence="3 4" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="2 3 4" id="KW-0413">Isomerase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193689" description="Triosephosphate isomerase" evidence="5">
        <location>
            <begin position="21"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="3">
        <location>
            <begin position="9"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="3">
        <location>
            <begin position="230"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="active site" description="Electrophile" evidence="3">
        <location>
            <position position="92"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="3">
        <location>
            <position position="164"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="170"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="209"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00147"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU363013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABF07811.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="245" mass="25353" checksum="0785E60BCA4A72DD" modified="2006-05-30" version="1">MRQKLVIGNWKMHGSLAANAALLEGIKAAPARAKLAVCAPFPYLAQCQTLLSGSQVAWGAQDVSSEARGAFTGEVAASMLGEFGCGYALVGHSERRTYHGETDAVVAAKALRALEFDIVPVVCVGETLAEREAGQTEAVVGRQLQAVLDALSIEQLGRIVLAYEPVWAIGTGKTATSAQAQEVHAALRARVTAKDAGVAARMAILYGGSVKPDNAAELFSMSDIDGGLIGGASLKADDFLAIGNA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-02-10" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LPZ1</accession>
    <name>Q1LPZ1_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Protozoan/cyanobacterial globin family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Rmet_0899</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07785.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0899"/>
    <dbReference type="EnsemblBacteria" id="ABF07785">
        <property type="protein sequence ID" value="ABF07785"/>
        <property type="gene ID" value="Rmet_0899"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0899"/>
    <dbReference type="eggNOG" id="COG2346">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_103526_3_0_4"/>
    <dbReference type="OMA" id="QYWGGPT"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019825">
        <property type="term" value="F:oxygen binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.490.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009050">
        <property type="entry name" value="Globin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012292">
        <property type="entry name" value="Globin/Proto"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001486">
        <property type="entry name" value="Hemoglobin_trunc"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01152">
        <property type="entry name" value="Bac_globin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46458">
        <property type="entry name" value="SSF46458"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0274">FAD</keyword>
    <keyword evidence="2" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABF07785.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="147" mass="17182" checksum="1C7253CB158F6D74" modified="2006-05-30" version="1">MSTESTNPPDGQSNQPEEQREQITAYELIGGEARVRELVDRFYDLMDLETEFAGLRALHPPSLEGSRDKLFWFLCGWLGGPNYFIERFGHPRLRARHLPFEIGTSERDQWMRCMALAMQDVGLSEDLQMRLMQALFQTADWMRNVQR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-03-24" modified="2022-08-03" version="100" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LQ72</accession>
    <name>SYQ_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Glutamine--tRNA ligase</fullName>
            <ecNumber evidence="1">6.1.1.18</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Glutaminyl-tRNA synthetase</fullName>
            <shortName evidence="1">GlnRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">glnS</name>
        <name type="ordered locus">Rmet_0818</name>
    </gene>
    <organism>
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + L-glutamine + tRNA(Gln) = AMP + diphosphate + L-glutaminyl-tRNA(Gln)</text>
            <dbReference type="Rhea" id="RHEA:20121"/>
            <dbReference type="Rhea" id="RHEA-COMP:9662"/>
            <dbReference type="Rhea" id="RHEA-COMP:9681"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78521"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.18"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-I aminoacyl-tRNA synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.18" evidence="1"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07704.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515648.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q1LQ72"/>
    <dbReference type="SMR" id="Q1LQ72"/>
    <dbReference type="STRING" id="266264.Rmet_0818"/>
    <dbReference type="EnsemblBacteria" id="ABF07704">
        <property type="protein sequence ID" value="ABF07704"/>
        <property type="gene ID" value="Rmet_0818"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0818"/>
    <dbReference type="eggNOG" id="COG0008">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001882_2_3_4"/>
    <dbReference type="OMA" id="INNFCAQ"/>
    <dbReference type="OrthoDB" id="142899at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004819">
        <property type="term" value="F:glutamine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006425">
        <property type="term" value="P:glutaminyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006424">
        <property type="term" value="P:glutamyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.240.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00126">
        <property type="entry name" value="Gln_tRNA_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001412">
        <property type="entry name" value="aa-tRNA-synth_I_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004514">
        <property type="entry name" value="Gln-tRNA-synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022861">
        <property type="entry name" value="Gln_tRNA_ligase_bac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000924">
        <property type="entry name" value="Glu/Gln-tRNA-synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020058">
        <property type="entry name" value="Glu/Gln-tRNA-synth_Ib_cat-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020059">
        <property type="entry name" value="Glu/Gln-tRNA-synth_Ib_codon-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020056">
        <property type="entry name" value="Rbsml_L25/Gln-tRNA_synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011035">
        <property type="entry name" value="Ribosomal_L25/Gln-tRNA_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00749">
        <property type="entry name" value="tRNA-synt_1c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03950">
        <property type="entry name" value="tRNA-synt_1c_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00987">
        <property type="entry name" value="TRNASYNTHGLU"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50715">
        <property type="entry name" value="SSF50715"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00440">
        <property type="entry name" value="glnS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00178">
        <property type="entry name" value="AA_TRNA_LIGASE_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_1000095506" description="Glutamine--tRNA ligase">
        <location>
            <begin position="1"/>
            <end position="582"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'HIGH' region" evidence="1">
        <location>
            <begin position="50"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'KMSKS' region" evidence="1">
        <location>
            <begin position="296"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="51"/>
            <end position="53"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="57"/>
            <end position="63"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="83"/>
        </location>
        <ligand>
            <name>L-glutamine</name>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="235"/>
        </location>
        <ligand>
            <name>L-glutamine</name>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="254"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="289"/>
            <end position="290"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00126"/>
        </source>
    </evidence>
    <sequence length="582" mass="66121" checksum="688FBD9447623404" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="58" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LQ76</accession>
    <name>Q1LQ76_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_0814</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07700.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515645.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0814"/>
    <dbReference type="EnsemblBacteria" id="ABF07700">
        <property type="protein sequence ID" value="ABF07700"/>
        <property type="gene ID" value="Rmet_0814"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0814"/>
    <dbReference type="HOGENOM" id="CLU_2275135_0_0_4"/>
    <dbReference type="OMA" id="RFPLWAV"/>
    <dbReference type="OrthoDB" id="1960953at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="46"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="76"/>
            <end position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF07700.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="102" mass="10693" checksum="1857C29E9800FE25" modified="2006-05-30" version="1">MSMIHLNDVVAASLAAAGACLACSIPADFLRRFPLWAYKTYGRGALLLPLLAVMVGTLIAGVAVEGDINSDGGIALVAAASFLGLILASALLRFWLADYRKR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-01-15" modified="2022-05-25" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LQD7</accession>
    <name>TRHO_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">tRNA uridine(34) hydroxylase</fullName>
            <ecNumber evidence="1">1.14.-.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">tRNA hydroxylation protein O</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">trhO</name>
        <name type="ordered locus">Rmet_0753</name>
    </gene>
    <organism>
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes oxygen-dependent 5-hydroxyuridine (ho5U) modification at position 34 in tRNAs.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>AH2 + O2 + uridine(34) in tRNA = 5-hydroxyuridine(34) in tRNA + A + H2O</text>
            <dbReference type="Rhea" id="RHEA:64224"/>
            <dbReference type="Rhea" id="RHEA-COMP:11727"/>
            <dbReference type="Rhea" id="RHEA-COMP:13381"/>
            <dbReference type="ChEBI" id="CHEBI:13193"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:17499"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="ChEBI" id="CHEBI:136877"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the TrhO family.</text>
    </comment>
    <dbReference type="EC" id="1.14.-.-" evidence="1"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07639.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515594.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q1LQD7"/>
    <dbReference type="SMR" id="Q1LQD7"/>
    <dbReference type="STRING" id="266264.Rmet_0753"/>
    <dbReference type="EnsemblBacteria" id="ABF07639">
        <property type="protein sequence ID" value="ABF07639"/>
        <property type="gene ID" value="Rmet_0753"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0753"/>
    <dbReference type="eggNOG" id="COG1054">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038878_0_1_4"/>
    <dbReference type="OMA" id="CDTHTNC"/>
    <dbReference type="OrthoDB" id="684577at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006400">
        <property type="term" value="P:tRNA modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.250.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00469">
        <property type="entry name" value="TrhO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001763">
        <property type="entry name" value="Rhodanese-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036873">
        <property type="entry name" value="Rhodanese-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020936">
        <property type="entry name" value="TrhO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040503">
        <property type="entry name" value="TRHO_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43268">
        <property type="entry name" value="PTHR43268"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17773">
        <property type="entry name" value="UPF0176_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00450">
        <property type="entry name" value="RHOD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52821">
        <property type="entry name" value="SSF52821"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50206">
        <property type="entry name" value="RHODANESE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0560">Oxidoreductase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0819">tRNA processing</keyword>
    <feature type="chain" id="PRO_1000013762" description="tRNA uridine(34) hydroxylase">
        <location>
            <begin position="1"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="domain" description="Rhodanese" evidence="1">
        <location>
            <begin position="128"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="active site" description="Cysteine persulfide intermediate" evidence="1">
        <location>
            <position position="182"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00469"/>
        </source>
    </evidence>
    <sequence length="282" mass="31384" checksum="F9D0DCC3E190E212" modified="2006-05-30" version="1">MQIVNISAYKFVTLEDIETLRPVMRERCDAAGLKGTILLAPEGINMFLAGTREQIDEFMAWLHADARFADIAPKESLSENQPFKRMLVRAKKEIITMKMPLIRPEAGRAPSVRPVDLKRWLDQGHDDDGRPVVMLDTRNAFEVAVGTFDKAVEYGIDKFSEFPPAVAENKAEFEGKTIVSFCTGGIRCEKAAIHMQEVGIDHVYQLEGGILKYFEEVGGSHYNGDCFVFDYRTALNPNLEPAGPVQCFACRAVVTPEEQQHPKYVVGKSCPHCVDTATQAAA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-10-31" modified="2022-08-03" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LQE0</accession>
    <name>TRMD_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">tRNA (guanine-N(1)-)-methyltransferase</fullName>
            <ecNumber evidence="1">2.1.1.228</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">M1G-methyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">tRNA [GM37] methyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">trmD</name>
        <name type="ordered locus">Rmet_0750</name>
    </gene>
    <organism>
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Specifically methylates guanosine-37 in various tRNAs.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>guanosine(37) in tRNA + S-adenosyl-L-methionine = H(+) + N(1)-methylguanosine(37) in tRNA + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:36899"/>
            <dbReference type="Rhea" id="RHEA-COMP:10145"/>
            <dbReference type="Rhea" id="RHEA-COMP:10147"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:73542"/>
            <dbReference type="ChEBI" id="CHEBI:74269"/>
            <dbReference type="EC" id="2.1.1.228"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the RNA methyltransferase TrmD family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.228" evidence="1"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07636.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515592.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q1LQE0"/>
    <dbReference type="SMR" id="Q1LQE0"/>
    <dbReference type="STRING" id="266264.Rmet_0750"/>
    <dbReference type="EnsemblBacteria" id="ABF07636">
        <property type="protein sequence ID" value="ABF07636"/>
        <property type="gene ID" value="Rmet_0750"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0750"/>
    <dbReference type="eggNOG" id="COG0336">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047363_0_2_4"/>
    <dbReference type="OMA" id="ILCGHYK"/>
    <dbReference type="OrthoDB" id="525632at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052906">
        <property type="term" value="F:tRNA (guanine(37)-N(1))-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd18080">
        <property type="entry name" value="TrmD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1270.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1280.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00605">
        <property type="entry name" value="TrmD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029028">
        <property type="entry name" value="Alpha/beta_knot_MTases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002649">
        <property type="entry name" value="tRNA_m1G_MeTrfase_bac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023148">
        <property type="entry name" value="tRNA_m1G_MeTrfase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029026">
        <property type="entry name" value="tRNA_m1G_MTases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016009">
        <property type="entry name" value="tRNA_MeTrfase_TRMD/TRM10"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46417">
        <property type="entry name" value="PTHR46417"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01746">
        <property type="entry name" value="tRNA_m1G_MT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000386">
        <property type="entry name" value="tRNA_mtase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75217">
        <property type="entry name" value="SSF75217"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00088">
        <property type="entry name" value="trmD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0489">Methyltransferase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <keyword id="KW-0819">tRNA processing</keyword>
    <feature type="chain" id="PRO_0000257454" description="tRNA (guanine-N(1)-)-methyltransferase">
        <location>
            <begin position="1"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="117"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="137"/>
            <end position="142"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00605"/>
        </source>
    </evidence>
    <sequence length="260" mass="28627" checksum="DC8F07B8032765D0" modified="2006-05-30" version="1">MQFDVITLFPEMFRALTDWGITSRAAKQQRYALRTWNPRDFTVDNYRTIDDRPYGGGPGMVMLAKPLEDAIDAAAAAQSDAGVGRPHVVLMSPQGATLTHEKVMALSQRPGLVLLCGRYEAIDQRLIDRRVDEEISLGDFVLSGGELPAMALIDAVVRHLPGVLGDAQSAVQDSFVNGLLDCPHYTRPEEYEGVRVPDVLLGGHHAEIEKWRRRQALANTARKRPDLIVAAREQGLLSKADEKFLSELAAKAGQEFAPAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LQE3</accession>
    <name>Q1LQE3_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative soluble quinoprotein glucose/sorbosone dehydrogenase</fullName>
            <ecNumber evidence="3">1.1.1.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_0747</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EC" id="1.1.1.-" evidence="3"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07633.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515589.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0747"/>
    <dbReference type="EnsemblBacteria" id="ABF07633">
        <property type="protein sequence ID" value="ABF07633"/>
        <property type="gene ID" value="Rmet_0747"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0747"/>
    <dbReference type="eggNOG" id="COG2133">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024435_3_1_4"/>
    <dbReference type="OMA" id="ETHHGWK"/>
    <dbReference type="OrthoDB" id="1150223at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011042">
        <property type="entry name" value="6-blade_b-propeller_TolB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012938">
        <property type="entry name" value="Glc/Sorbosone_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011041">
        <property type="entry name" value="Quinoprot_gluc/sorb_DH"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07995">
        <property type="entry name" value="GSDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50952">
        <property type="entry name" value="SSF50952"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193701" evidence="1">
        <location>
            <begin position="33"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="domain" description="GSDH" evidence="2">
        <location>
            <begin position="145"/>
            <end position="369"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF07633.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="377" mass="41588" checksum="4E410C6282A90963" modified="2006-05-30" version="1">MRPNRHTAQYLAKSLCRLALLGAAWSAATAQAALPIDQIRLPPGFKIEVLSDDVPGARAMTLSPKGTLFVGSRAEGKVYALESPLGPHPKMRVIASGLTMPVGVAFHDGALYASSTSRIVRLDNIEGRLGDPPRPVTISDRFPTETHHGWKFIAFGPDGYLYVPVGAPCNICAPDENRYANIMKMKPDGSDLRVVARGVRNTVGFDWHPATHELWFTDNGRDWLGDDQPDDELNRLTREGEHFGFPYCHAGTIADPEFGKQRPCSAFTPPVAKLGPHVAALGMRFYTGTQFPPEYRKNNIFIAEHGSWNRSTAIGYRIARIVLDDDGRVIRQEIFAEGWLRDGKAWGRPVDVQVASDGSLLVSDDKANAIYRIRYAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="112" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LQK6</accession>
    <name>Q1LQK6_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Thioredoxin reductase</fullName>
            <ecNumber evidence="3">1.8.1.9</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">trxB</name>
        <name evidence="6" type="ordered locus">Rmet_0684</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>[thioredoxin]-dithiol + NADP(+) = [thioredoxin]-disulfide + H(+) + NADPH</text>
            <dbReference type="Rhea" id="RHEA:20345"/>
            <dbReference type="Rhea" id="RHEA-COMP:10698"/>
            <dbReference type="Rhea" id="RHEA-COMP:10700"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:50058"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.8.1.9"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="4">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
        <text evidence="4">Binds 1 FAD per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the class-II pyridine nucleotide-disulfide oxidoreductase family.</text>
    </comment>
    <dbReference type="EC" id="1.8.1.9" evidence="3"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07570.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515537.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0684"/>
    <dbReference type="EnsemblBacteria" id="ABF07570">
        <property type="protein sequence ID" value="ABF07570"/>
        <property type="gene ID" value="Rmet_0684"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0684"/>
    <dbReference type="eggNOG" id="COG0492">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031864_5_1_4"/>
    <dbReference type="OMA" id="FRAEDYW"/>
    <dbReference type="OrthoDB" id="692968at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004791">
        <property type="term" value="F:thioredoxin-disulfide reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019430">
        <property type="term" value="P:removal of superoxide radicals"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023753">
        <property type="entry name" value="FAD/NAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008255">
        <property type="entry name" value="Pyr_nucl-diS_OxRdtase_2_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005982">
        <property type="entry name" value="Thioredox_Rdtase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07992">
        <property type="entry name" value="Pyr_redox_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="SSF51905"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01292">
        <property type="entry name" value="TRX_reduct"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00573">
        <property type="entry name" value="PYRIDINE_REDOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0274">FAD</keyword>
    <keyword evidence="3" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="4" id="KW-0521">NADP</keyword>
    <keyword evidence="3 6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="2 3" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pyr_redox_2" evidence="5">
        <location>
            <begin position="7"/>
            <end position="299"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF07992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABF07570.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="317" mass="33990" checksum="71B8296B21832052" modified="2006-05-30" version="1">MSAKHAKVLILGSGPAGYTAAVYAARANLNPVLITGLAQGGQLMTTTDVENWPADPNGVQGPDLMQRFLAHAERFNTEIIFDHIHTAKLTEKPIRLIGDSGEYTCDALIIATGASAQYLGLPSEEAFMGRGVSACATCDGFFYKNQEVAVIGGGNTAVEEALYLSHIASKVTLIHRRDKFRAEPILVDRLMQRVEEGRIDLRYDSTLDEVLGDESGVTGLRIRDTKDAAKTEDLKLAGVFVAIGHKPNTDLFEGQLEMKNGYIVTKSGLSGDATATNIPGVFAAGDVQDHVYRQAITSAGTGCMAALDAQRYVEALK</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="52" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LQL4</accession>
    <name>Q1LQL4_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_0676</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07562.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008643588.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0676"/>
    <dbReference type="EnsemblBacteria" id="ABF07562">
        <property type="protein sequence ID" value="ABF07562"/>
        <property type="gene ID" value="Rmet_0676"/>
    </dbReference>
    <dbReference type="GeneID" id="60825154"/>
    <dbReference type="KEGG" id="rme:Rmet_0676"/>
    <dbReference type="HOGENOM" id="CLU_2603478_0_0_4"/>
    <dbReference type="OMA" id="RIHWCLY"/>
    <dbReference type="OrthoDB" id="1826937at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="51"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF07562.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="79" mass="9168" checksum="365D6B5D8537D175" modified="2006-05-30" version="1">MGNINFLSVAFAIFFFWMAWHVRNKRATNPSGMPRLQVFKRKWGESLGDRIHWCLYVALPFLLAIMMVGNALRGKGPFH</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LQL9</accession>
    <name>Q1LQL9_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Type IV pilus signal transduction protein PilI</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">pilI</name>
        <name evidence="2" type="ordered locus">Rmet_0671</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07557.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515527.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0671"/>
    <dbReference type="EnsemblBacteria" id="ABF07557">
        <property type="protein sequence ID" value="ABF07557"/>
        <property type="gene ID" value="Rmet_0671"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0671"/>
    <dbReference type="eggNOG" id="COG0835">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_048995_6_0_4"/>
    <dbReference type="OMA" id="PLTQPWY"/>
    <dbReference type="OrthoDB" id="1711729at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006935">
        <property type="term" value="P:chemotaxis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036061">
        <property type="entry name" value="CheW-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002545">
        <property type="entry name" value="CheW-lke_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01584">
        <property type="entry name" value="CheW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00260">
        <property type="entry name" value="CheW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50341">
        <property type="entry name" value="SSF50341"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50851">
        <property type="entry name" value="CHEW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CheW-like" evidence="1">
        <location>
            <begin position="35"/>
            <end position="172"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50851"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF07557.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="180" mass="19814" checksum="63B3336DC962FA43" modified="2006-05-30" version="1">MNAPRQDLRARQTRLQDYQAMLARRLRDARNLPAVDSYLGVVVGQRNWLLPLLETGEVIEMRQPAGVPLTQPWYRGLVNARGNLLGVIDFAMFCGDGPTPVQPGSKIVVLSRHVERACGIIATRVLGLRHAVDLTPAQGDEPEGWQGLRFADKEGRGWQVLDIGQLLASPAFLQAGRTVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="100" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LR46</accession>
    <name>Q1LR46_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Superfamily II DNA/RNA helicase, SNF2 family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Rmet_0494</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07380.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0494"/>
    <dbReference type="EnsemblBacteria" id="ABF07380">
        <property type="protein sequence ID" value="ABF07380"/>
        <property type="gene ID" value="Rmet_0494"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0494"/>
    <dbReference type="eggNOG" id="COG0553">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000315_21_7_4"/>
    <dbReference type="OMA" id="FSQFVGH"/>
    <dbReference type="OrthoDB" id="325071at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140658">
        <property type="term" value="F:ATP-dependent chromatin remodeler activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10810">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038718">
        <property type="entry name" value="SNF2-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000330">
        <property type="entry name" value="SNF2_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00176">
        <property type="entry name" value="SNF2-rel_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 5" id="KW-0347">Helicase</keyword>
    <keyword evidence="1 5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="3">
        <location>
            <begin position="565"/>
            <end position="721"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="4">
        <location>
            <begin position="856"/>
            <end position="1019"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="36"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABF07380.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="1025" mass="111132" checksum="CF822DAE9DCED104" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LRL3</accession>
    <name>Q1LRL3_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Permease of the Major Facilitator Family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">arsP</name>
        <name evidence="7" type="ordered locus">Rmet_0327</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07213.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515208.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0327"/>
    <dbReference type="TCDB" id="2.A.1.70.8">
        <property type="family name" value="the major facilitator superfamily (mfs)"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF07213">
        <property type="protein sequence ID" value="ABF07213"/>
        <property type="gene ID" value="Rmet_0327"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0327"/>
    <dbReference type="eggNOG" id="COG2814">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001265_59_0_4"/>
    <dbReference type="OMA" id="IGWRGAC"/>
    <dbReference type="OrthoDB" id="1594866at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="SSF103473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="7"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="44"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="74"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="99"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="132"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="158"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="217"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="243"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="284"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="369"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="domain" description="MFS" evidence="6">
        <location>
            <begin position="7"/>
            <end position="391"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABF07213.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="397" mass="40512" checksum="C9432D450A6B6802" modified="2006-05-30" version="1">MEPSSSVIARLGVAQTLAWGSTYYLPAMLATPMAQDLGVSTPTIFAAFSVALVVSALLGPAAGRMIDRTGGRSVLVATSLVFTIGLARLGVAQGVVGLFAAWVILGIGMGAGLYEAAFATLVALYGNRSRGAITGITLIAGFASTVGWPLSTSMEAHIGWRGACLSWAALHLTLGLALNLSIPRVAATATAPSRPTSQTGSALTHHADERSQRTASYALAFVFAATWFISTAMAAHLPRLLQAGGASLATAVMVGALIGPAQVGARILEFGLLRKVHPLLSARIAAALHPLGALAFGLVGAPAAALFGVLHGAGNGILTIAKGTLPLVIFGPGGYGHRQGVLMVPARVAQALAPWIFGMCLDQFGAKSLWLSSALGMMAILTLMAVRQSAPLKTSTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LRM6</accession>
    <name>Q1LRM6_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Putative transporter, major facilitator family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Rmet_0314</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
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        <property type="protein sequence ID" value="ABF07200.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515200.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0314"/>
    <dbReference type="EnsemblBacteria" id="ABF07200">
        <property type="protein sequence ID" value="ABF07200"/>
        <property type="gene ID" value="Rmet_0314"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0314"/>
    <dbReference type="eggNOG" id="COG2814">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001265_10_0_4"/>
    <dbReference type="OMA" id="LYADSFH"/>
    <dbReference type="OrthoDB" id="626204at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="SSF103473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00216">
        <property type="entry name" value="SUGAR_TRANSPORT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="34"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="69"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="102"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="126"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="158"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="189"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="231"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="268"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="293"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="317"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="355"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="382"/>
            <end position="400"/>
        </location>
    </feature>
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        <location>
            <begin position="34"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ABF07200.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="418" mass="44247" checksum="3C4915265C87A9B7" modified="2006-05-30" version="1">MPSIPPSSQPASLADESTRTEPARDRMTKGELRASLTLASIFALRMLGLFLILPVFAEYARTLPDGHDAQRVGIAMGIYGLMQAFLHIPLGWLSDRVGRKPVMVAGLLLFVAGALVAAFSDTLTGIIIGRALQGMGAISAAITACIADLTRERHRTKAMAMVGGSIGLTFALSLVIASPLLHSIGMSGIFSLMAVFGVVAIGVTLFLVPAPPPPHPVRLPFRQVLMNADLIRLNVGVLALHASQVAMFMVVPAMLSDAGMPLDQHWKVYLPVVLVSFLLMLAPMMAAERYGKVRPVLLASVALMTVVQILFASVHGLWPIVGVLLLFFVAFNVLEAMQPSLVSRYAAVSRGAALGVYNTTQSLGLFLGGVAGGWLLQHEGRSAVFIGCAVVLVLWLIIAWSMKAPPARSQGSASAVAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-10-07" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LRN9</accession>
    <name>Q1LRN9_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_0301</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07187.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0301"/>
    <dbReference type="EnsemblBacteria" id="ABF07187">
        <property type="protein sequence ID" value="ABF07187"/>
        <property type="gene ID" value="Rmet_0301"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0301"/>
    <dbReference type="HOGENOM" id="CLU_2357664_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="39"/>
            <end position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF07187.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="96" mass="10903" checksum="61CA1F94C5BAA059" modified="2006-05-30" version="1">MMGARRTRRWRTRYPSTSPATFGQPGVFATDAARCMLRHTTTTDRTAPDNRPGIPRGKRSRDGGWQFSGWAIRPASSRHHSRRRRRSAQVRSGPAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="98" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LRR5</accession>
    <name>Q1LRR5_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Nitrate ATP-binding ABC transporter protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">nasD</name>
        <name evidence="9" type="ordered locus">Rmet_0275</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07161.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515164.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0275"/>
    <dbReference type="EnsemblBacteria" id="ABF07161">
        <property type="protein sequence ID" value="ABF07161"/>
        <property type="gene ID" value="Rmet_0275"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0275"/>
    <dbReference type="eggNOG" id="COG1116">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_22_4"/>
    <dbReference type="OMA" id="LPWLTCF"/>
    <dbReference type="OrthoDB" id="1832232at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015112">
        <property type="term" value="F:nitrate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005890">
        <property type="entry name" value="NO3_transporter_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01184">
        <property type="entry name" value="ntrCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 7 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="1" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-1278">Translocase</keyword>
    <feature type="domain" description="ABC transporter" evidence="8">
        <location>
            <begin position="5"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="41"/>
            <end position="48"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ABF07161.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="267" mass="29066" checksum="EF253C482035E820" modified="2006-05-30" version="1">MEKFVSIENVGQTFKTRKGPFVALRDIDLSIAEGEFVTLIGHSGCGKSTLLNLIAGLTAPTTGALICAGREIAGPGPERAVVFQNHSLLPWMTCFENVHLGVERVFGQSETPAQLKARTHDTLALVGLTHAESKYPHEISGGMKQRVGIARALAMQPKVLLMDEPFGALDALTRAHLQDALLKIVARTRSTVVMVTHDVDEAVLLADRIVMMTNGPAATIGEILRVDIPRPRDRVTLADDKTFHACRTAVLDFLYRKQRNPALEEAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="72" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LS46</accession>
    <name>Q1LS46_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Methionine biosynthesis protein MetW</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">metW</name>
        <name evidence="1" type="ordered locus">Rmet_0144</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07030.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008647215.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0144"/>
    <dbReference type="EnsemblBacteria" id="ABF07030">
        <property type="protein sequence ID" value="ABF07030"/>
        <property type="gene ID" value="Rmet_0144"/>
    </dbReference>
    <dbReference type="GeneID" id="60822867"/>
    <dbReference type="KEGG" id="rme:Rmet_0144"/>
    <dbReference type="eggNOG" id="COG2226">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_091323_0_0_4"/>
    <dbReference type="OMA" id="LPYEWYN"/>
    <dbReference type="OrthoDB" id="957193at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010743">
        <property type="entry name" value="Methionine_synth_MetW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07021">
        <property type="entry name" value="MetW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02081">
        <property type="entry name" value="metW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF07030.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="203" mass="22735" checksum="5AA1D9442DACFFAE" modified="2006-05-30" version="1">MNAASNPNILALRPDFRAIARWIEPNSTVLDAGCGDGSLLRVLQDELDVQAYGIEIRDEEVLACAQKGVHVIQQNLEGGLALFEDQSFDTVILSQTLQTIHNTAQVLRDTLRVGRECIVSFPNFGYWPHRLSVFRGRMPVSESLPYQWYNTPNVRVLTISDFEALAPKVGLRVLDRVVMHEGVTVNWGVNWRGSLAVYRVCAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LS80</accession>
    <name>Q1LS80_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">HAD-superfamily hydrolase, subfamily IA</fullName>
            <ecNumber evidence="1">3.1.3.18</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_0110</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EC" id="3.1.3.18" evidence="1"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF06996.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515030.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0110"/>
    <dbReference type="EnsemblBacteria" id="ABF06996">
        <property type="protein sequence ID" value="ABF06996"/>
        <property type="gene ID" value="Rmet_0110"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0110"/>
    <dbReference type="eggNOG" id="COG0637">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045011_13_2_4"/>
    <dbReference type="OMA" id="MDVYGFT"/>
    <dbReference type="OrthoDB" id="1167152at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008967">
        <property type="term" value="F:phosphoglycolate phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.240">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006439">
        <property type="entry name" value="HAD-SF_hydro_IA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041492">
        <property type="entry name" value="HAD_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023198">
        <property type="entry name" value="PGP-like_dom2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13419">
        <property type="entry name" value="HAD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="SSF56784"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01509">
        <property type="entry name" value="HAD-SF-IA-v3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF06996.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="227" mass="24651" checksum="7EEB42723BAF6576" modified="2006-05-30" version="1">MTAANQAARFDCVIFDCDGVLVDSEPIVHRVLNRVLNEIGIEITLEESMKWFLGRAVRDELGNIEARLGKPLPRNFLSEWFVRRDAALIEELEAVPHVRQAIEAVKARGLPVCVASGADRIKVKLQLNHTQLVELFTDDAAREHIFSATEVEHSKPAPDVYLLAARTMGVDPSRCAVIEDSPTGATAGVAAGMTVFGYAASTNADALRAVGVTTIFTDMRELPGLIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="96" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LSF0</accession>
    <name>Q1LSF0_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Potassium-transporting ATPase KdpC subunit</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">ATP phosphohydrolase [potassium-transporting] C chain</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">Potassium-binding and translocating subunit C</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">Potassium-translocating ATPase C chain</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11 12" type="primary">kdpC</name>
        <name evidence="12" type="ordered locus">Rmet_0040</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Part of the high-affinity ATP-driven potassium transport (or Kdp) system, which catalyzes the hydrolysis of ATP coupled with the electrogenic transport of potassium into the cytoplasm. This subunit acts as a catalytic chaperone that increases the ATP-binding affinity of the ATP-hydrolyzing subunit KdpB by the formation of a transient KdpB/KdpC/ATP ternary complex.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">The system is composed of three essential subunits: KdpA, KdpB and KdpC.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="11">Cell inner membrane</location>
            <topology evidence="11">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the KdpC family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF06926.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011514967.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0040"/>
    <dbReference type="EnsemblBacteria" id="ABF06926">
        <property type="protein sequence ID" value="ABF06926"/>
        <property type="gene ID" value="Rmet_0040"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0040"/>
    <dbReference type="eggNOG" id="COG2156">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_077094_2_0_4"/>
    <dbReference type="OMA" id="KYFWPRP"/>
    <dbReference type="OrthoDB" id="1912405at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005887">
        <property type="term" value="C:integral component of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008556">
        <property type="term" value="F:P-type potassium transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00276">
        <property type="entry name" value="KdpC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003820">
        <property type="entry name" value="KdpC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30042">
        <property type="entry name" value="PTHR30042"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02669">
        <property type="entry name" value="KdpC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001296">
        <property type="entry name" value="K_ATPase_KdpC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00681">
        <property type="entry name" value="kdpC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="2 11" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9 11" id="KW-0406">Ion transport</keyword>
    <keyword evidence="10 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7 11" id="KW-0630">Potassium</keyword>
    <keyword evidence="3 11" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="1 11" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="21"/>
            <end position="39"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00276"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ABF06926.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="203" mass="20828" checksum="9E84C673730E8B5E" modified="2006-05-30" version="1">MTTQSNTVAQVPRQTGLLRPALTVFIVLSVVTGLLYPGVVTGIARAVFPHQAAGSLIVKDGKTVGSELIGQPFSDPKYFWGRLSATAPMSYNATASGGSNLGPTNPALTDAAKARIDGLREADPDNKAPVPVDLVTASGSGLDPHISPAAAQYQAARVARLRGVSLEKVEALIAANTKDPGLAVLGEPAVNVLALNLALDGLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-04-26" modified="2022-01-19" version="95" xmlns="http://uniprot.org/uniprot">
    <accession>Q58AE8</accession>
    <name>Q58AE8_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Glycosyltransferase (Bactoprenol) involved in cell wall biogenesis family 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">gtrB2</name>
        <name evidence="4" type="ordered locus">Rmet_6128</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4 5">
        <name>pMOL30</name>
    </geneLocation>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="5">Plasmid pMOL30</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000354">
        <property type="protein sequence ID" value="ABF12987.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011229396.1">
        <property type="nucleotide sequence ID" value="NC_007971.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_145670.1">
        <property type="nucleotide sequence ID" value="NC_006466.1"/>
    </dbReference>
    <dbReference type="CAZy" id="GT2">
        <property type="family name" value="Glycosyltransferase Family 2"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF12987">
        <property type="protein sequence ID" value="ABF12987"/>
        <property type="gene ID" value="Rmet_6128"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6128"/>
    <dbReference type="HOGENOM" id="CLU_033536_0_1_4"/>
    <dbReference type="OMA" id="IVVPMYF"/>
    <dbReference type="OrthoDB" id="1064289at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid pMOL30"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001173">
        <property type="entry name" value="Glyco_trans_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00535">
        <property type="entry name" value="Glycos_transf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="SSF53448"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0614">Plasmid</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0808">Transferase</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="236"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="269"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="domain" description="Glyco_trans_2-like" evidence="3">
        <location>
            <begin position="12"/>
            <end position="173"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF12987.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="367" mass="40791" checksum="22CE0A3EB7285ED7" modified="2005-04-26" version="1">MSSDKRLQGTVSVVVPIFNEEQVLPLFHARLGAVLGTLPYTSEVIYVDDGSRDRTLDVLRTIRGADEKVGIIELSRNFGKEAALTAGLDVACGDAVVVIDADLQDPPELIPTLINKWEQGFDVVYATRRTREGESFLKKATAHCFYRLMGKVSDIDIPADTGDFRLMSQRAVAAIGQLREQNRFMKGLYAWIGYRQTSILYDRDARASGKTKFNYRRLLKLAIDGVTGFSTIPLRIATSVGLIVAVPAFWMALYTVGKTLLFGDPVRGYPSLMTVVLMLGGLQLFFLGILGEYVGRLFNEAKRRPLYLVAGYTPAREMDEEGIPFRRVSDATRSMDVVVRAVRRADSVEHSPAPLDIASSEVRKNWT</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-04-26" modified="2021-06-02" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>Q58AQ3</accession>
    <name>Q58AQ3_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_6015</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="1 2">
        <name>pMOL30</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid pMOL30</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000354">
        <property type="protein sequence ID" value="ABF12874.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011229316.1">
        <property type="nucleotide sequence ID" value="NC_007971.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_145562.1">
        <property type="nucleotide sequence ID" value="NC_006466.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF12874">
        <property type="protein sequence ID" value="ABF12874"/>
        <property type="gene ID" value="Rmet_6015"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6015"/>
    <dbReference type="HOGENOM" id="CLU_1271422_0_0_4"/>
    <dbReference type="OMA" id="KECWLAG"/>
    <dbReference type="OrthoDB" id="2021077at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid pMOL30"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF12874.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="217" mass="24159" checksum="49D47229984A2998" modified="2005-04-26" version="1">MQDQFYVLYSPTEAAALTDATPGEHGAGFWSAEDGWSSFDKATRYAAGLREILPFPKSLGDDAGWVLVDSILFLALAKRYSEDPQLSEQLDRLADRYADEVIGGHPERFNSVEIQGVRNEHSPRDAGGGIVELDNVDPQFYSVYLHYKPEEECGSDCVGDFATYEGAVQYANELAEENGWAILDLVTEHGRTPRWEPSHSVPRVLPVRGRHDLSVQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-03-23" modified="2021-06-02" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>D3DXL4</accession>
    <name>D3DXL4_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_6416</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ADC45034.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515307.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_6416"/>
    <dbReference type="EnsemblBacteria" id="ADC45034">
        <property type="protein sequence ID" value="ADC45034"/>
        <property type="gene ID" value="Rmet_6416"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6416"/>
    <dbReference type="eggNOG" id="COG4392">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_157896_1_2_4"/>
    <dbReference type="OrthoDB" id="2078961at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008407">
        <property type="entry name" value="Brnchd-chn_aa_trnsp_AzlD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05437">
        <property type="entry name" value="AzlD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="36"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="74"/>
            <end position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADC45034.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="107" mass="11389" checksum="689835EEEC0426F0" modified="2010-03-23" version="1">MSQMTLLWVFIGAGLATFLIRLSFIAVEGRVRLPAWFRTALQFVPAAMLSALIAPDLLMRDGAVFVSPHNARLIAGVVAIVIAARTRSVGWTIAGGMAALIALEALI</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-03-23" modified="2020-10-07" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>D3DXR6</accession>
    <name>D3DXR6_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_6478</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ADC45086.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_6478"/>
    <dbReference type="EnsemblBacteria" id="ADC45086">
        <property type="protein sequence ID" value="ADC45086"/>
        <property type="gene ID" value="Rmet_6478"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6478"/>
    <dbReference type="HOGENOM" id="CLU_2993478_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADC45086.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="57" mass="6271" checksum="8131DD03A1B04CDC" modified="2010-03-23" version="1">MICFSPFPHSGRGAGERDSAPPGQLRKTKPLPSPKPSPAMREREQTGCIFNTRGDFQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-03-23" modified="2020-10-07" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>D3DXS8</accession>
    <name>D3DXS8_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_6490</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ADC45098.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_6490"/>
    <dbReference type="EnsemblBacteria" id="ADC45098">
        <property type="protein sequence ID" value="ADC45098"/>
        <property type="gene ID" value="Rmet_6490"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6490"/>
    <dbReference type="HOGENOM" id="CLU_1915340_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="23"/>
            <end position="46"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADC45098.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="132" mass="14387" checksum="21BED0BB7CCAC1F2" modified="2010-03-23" version="1">MCLTSVARESQAALLRQIRDMPRASGQSGAAHGAAAPTHLSHLRRRSPSRYRNAIGGAFAGNVGWHKLRVNCDARIYRRCGTDNGLERQGTVHQLSACTGKAQSKSSFILASNLGFGLGRHRFAHAGRFEFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-03-23" modified="2020-10-07" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>D3DXW4</accession>
    <name>D3DXW4_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_6527</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ADC45134.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_6527"/>
    <dbReference type="EnsemblBacteria" id="ADC45134">
        <property type="protein sequence ID" value="ADC45134"/>
        <property type="gene ID" value="Rmet_6527"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6527"/>
    <dbReference type="HOGENOM" id="CLU_3423057_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADC45134.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="23" mass="2698" checksum="BB5857EB089E2460" modified="2010-03-23" version="1">MNATKAAWQADFRIELPTKHIKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-03-23" modified="2021-06-02" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>D3DY73</accession>
    <name>D3DY73_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_6643</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ADC45243.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_6643"/>
    <dbReference type="EnsemblBacteria" id="ADC45243">
        <property type="protein sequence ID" value="ADC45243"/>
        <property type="gene ID" value="Rmet_6643"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6643"/>
    <dbReference type="HOGENOM" id="CLU_2438614_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="54"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="63"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADC45243.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="90" mass="9742" checksum="D65591C727E8D752" modified="2010-03-23" version="1">MAQPVDPSRRGRITPRCVDAPGFGHGYGPDWSTLATTLQIGNEIGLRVMAQQQLARASAGGSGSRRGERREASRHEMAHYFSVGGEQSLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-03-23" modified="2020-10-07" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>D3DY96</accession>
    <name>D3DY96_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_6678</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ADC45266.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_6678"/>
    <dbReference type="EnsemblBacteria" id="ADC45266">
        <property type="protein sequence ID" value="ADC45266"/>
        <property type="gene ID" value="Rmet_6678"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6678"/>
    <dbReference type="HOGENOM" id="CLU_2495633_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADC45266.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="86" mass="9240" checksum="6934F9959405FEE4" modified="2010-03-23" version="1">MGRPGILALPELGHLNFSLAAQGQECRLMAGKSLIDQDIFRACRRRLESTHLRPSAVPSQVTGADASAVVRALPQVESAVQKVLPH</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-03-23" modified="2020-10-07" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>D3DYA4</accession>
    <name>D3DYA4_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_6689</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ADC45274.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_6689"/>
    <dbReference type="EnsemblBacteria" id="ADC45274">
        <property type="protein sequence ID" value="ADC45274"/>
        <property type="gene ID" value="Rmet_6689"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6689"/>
    <dbReference type="HOGENOM" id="CLU_2510301_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADC45274.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="85" mass="9244" checksum="DBBA765ADF924EA0" modified="2010-03-23" version="1">MCQFATCPSWCLCGPPWTIPAIAKSARGNPSGRIWIPDLFSGPTTDDILTLQYFGNKTLPSLIPHTWIRSTLISVASVIVAPSTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-03-23" modified="2020-02-26" version="17" xmlns="http://uniprot.org/uniprot">
    <accession>D3DYB9</accession>
    <name>D3DYB9_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_6707</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ADC45289.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADC45289">
        <property type="protein sequence ID" value="ADC45289"/>
        <property type="gene ID" value="Rmet_6707"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6707"/>
    <dbReference type="HOGENOM" id="CLU_2809364_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADC45289.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="67" mass="7402" checksum="32C9424A01C2D4FC" modified="2010-03-23" version="1">MRRSVAERAPFVDLAVAPVYQQLSPCSLRQLRDDVDGRLSCLIPRAGSRPFDCDGPLTAVREDMNVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="56" xmlns="http://uniprot.org/uniprot">
    <accession>Q1L9S8</accession>
    <name>Q1L9S8_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_6239</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="1 2">
        <name>pMOL28</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid pMOL28</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000355">
        <property type="protein sequence ID" value="ABF13098.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011514885.1">
        <property type="nucleotide sequence ID" value="NC_007972.2"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF13098">
        <property type="protein sequence ID" value="ABF13098"/>
        <property type="gene ID" value="Rmet_6239"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6239"/>
    <dbReference type="HOGENOM" id="CLU_199579_1_0_4"/>
    <dbReference type="OMA" id="YRFESVM"/>
    <dbReference type="OrthoDB" id="2035597at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid pMOL28"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032866">
        <property type="entry name" value="Prok_Ub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022289">
        <property type="entry name" value="PRTRC_protein-C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14454">
        <property type="entry name" value="Prok_Ub"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03738">
        <property type="entry name" value="PRTRC_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF13098.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="74" mass="8035" checksum="D6BA7185A0D67E07" modified="2006-05-30" version="1">MALEIVKLIRQFSYNGMTFMDPGAAFTPDQVRDVYSAQYPELTTAAVDGPEVKDGVARFTFVRAAGAKGAYAQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="72" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LAS6</accession>
    <name>Q1LAS6_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Fe2+ transport system protein A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">feoA</name>
        <name evidence="4" type="ordered locus">Rmet_5891</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="5">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="5">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the FeoA family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12750.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518099.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5891"/>
    <dbReference type="EnsemblBacteria" id="ABF12750">
        <property type="protein sequence ID" value="ABF12750"/>
        <property type="gene ID" value="Rmet_5891"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5891"/>
    <dbReference type="eggNOG" id="COG1918">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_150646_1_0_4"/>
    <dbReference type="OMA" id="VQIIAYG"/>
    <dbReference type="OrthoDB" id="2047422at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046914">
        <property type="term" value="F:transition metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007167">
        <property type="entry name" value="Fe-transptr_FeoA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038157">
        <property type="entry name" value="FeoA_core_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008988">
        <property type="entry name" value="Transcriptional_repressor_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04023">
        <property type="entry name" value="FeoA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00899">
        <property type="entry name" value="FeoA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50037">
        <property type="entry name" value="SSF50037"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0614">Plasmid</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FeoA" evidence="3">
        <location>
            <begin position="1"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF12750.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="100" mass="10804" checksum="D4C03C8440E35D88" modified="2006-05-30" version="1">MRLSELPRRTAAVVKSVDDEFTHDPIARRLRDLGFVPGETVQIIAFGPFGKDPLVAQVGYSRFALRRSEAARITVEVASATVTSIAPAGETEAPPSQRIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-10-07" version="67" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LB68</accession>
    <name>Q1LB68_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative transcriptional regulator (PadR-like family)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_5749</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12608.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5749"/>
    <dbReference type="EnsemblBacteria" id="ABF12608">
        <property type="protein sequence ID" value="ABF12608"/>
        <property type="gene ID" value="Rmet_5749"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5749"/>
    <dbReference type="eggNOG" id="COG1695">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2207905_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005149">
        <property type="entry name" value="Tscrpt_reg_PadR_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03551">
        <property type="entry name" value="PadR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PadR" evidence="1">
        <location>
            <begin position="38"/>
            <end position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF12608.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="127" mass="14303" checksum="3B08F04D72E7606F" modified="2006-05-30" version="1">MCSRVNHPPPYSVASDMKRQFLGLLARVYVMQFAMEVPATVAMLAEMLMDYGFQVDIGSVRPLLRALQMEGYLESVLRDGIGRVYLLTELGREELATSRTRIDELYRRLHNPVAETVAMAPATVSHT</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LBL3</accession>
    <name>Q1LBL3_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">HDOD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_5604</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12463.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011520011.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5604"/>
    <dbReference type="EnsemblBacteria" id="ABF12463">
        <property type="protein sequence ID" value="ABF12463"/>
        <property type="gene ID" value="Rmet_5604"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5604"/>
    <dbReference type="eggNOG" id="COG1639">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018569_0_0_4"/>
    <dbReference type="OMA" id="YWTKRIS"/>
    <dbReference type="OrthoDB" id="935550at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029016">
        <property type="entry name" value="GAF-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013976">
        <property type="entry name" value="HDOD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08668">
        <property type="entry name" value="HDOD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51833">
        <property type="entry name" value="HDOD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HDOD" evidence="1">
        <location>
            <begin position="20"/>
            <end position="215"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF12463.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="480" mass="52435" checksum="48BF241A453FB1AB" modified="2006-05-30" version="1">MSKSDFFETLWARMAQQGDFPTLQYCVDNIFKTLNADLTVGEMATSVLSDFSLSQKVIRLANSAMYRSFGGEVTTVSRAILVLGVEAISHLTLGIQLLDYFHGVAPNRPQATRALQRALVAGEITRALSQARGINEGEEAVVCTLMHHMSRLLIVFYFPEEWERVEAMCAAHPMTENEACQEVLGVSMVEIAEAAARKWRLPGLIAHAMKPRALSTDTVLETHADWLAAIAQVSAQAAATMVRDGNQASVEALLLSHAKVLGLDASDVSVAVRQAEVLNASIVESESPAQDATTLAHGARPDNVLERLRQGIAEVQREGAGRTVSQLAPMVLESSMRALDFSRCFLMMLNPSTRHFHARLGFGEGMRERLSALSFEEGFVPDVFHFAALASKPLLVEDTTDAEVARRLPRWYRDAIPDARSILLAPVRIRNRCIAMVYGDWGTARAPAGLGEAELETVDELMREIAAGVLRAAPAQSPSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LBP1</accession>
    <name>Q1LBP1_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative 2-aminoadipate transaminase</fullName>
            <ecNumber evidence="2">2.6.1.39</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_5576</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EC" id="2.6.1.39" evidence="2"/>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12435.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519985.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5576"/>
    <dbReference type="EnsemblBacteria" id="ABF12435">
        <property type="protein sequence ID" value="ABF12435"/>
        <property type="gene ID" value="Rmet_5576"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5576"/>
    <dbReference type="eggNOG" id="COG1167">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017584_0_6_4"/>
    <dbReference type="OMA" id="WRKIYKH"/>
    <dbReference type="OrthoDB" id="1024978at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047536">
        <property type="term" value="F:2-aminoadipate transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009058">
        <property type="term" value="P:biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="SSF53383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aminotran_1_2" evidence="1">
        <location>
            <begin position="59"/>
            <end position="392"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF12435.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="407" mass="43372" checksum="75FBBCC5816BB23C" modified="2006-05-30" version="1">MAAYPFVPALREPQGSPIRELFKYLSDPEMISFAGGYPSAALFDVEGIGAASAQALRERPAECLQYGATEGTPALRNALAALMAERGAAVALDELLITSGSQQGFDFLVRALVEPGSLVLVEEPTYSATLQALRLAGADVRGVPSDHDGMNVDALEAMLADAPARPRMIYTVPTFANPTGATLSPARRLQLLELVARHEIVLVEDDPYGALSFDGPPPPSLLALCDQVSGARPWLVHLASLSKTLAPGLRLGWMVAAPEIVRRAVIAKQVSDLCTPPWLQLTAAHYLQAGNLPGQIAREVAVYRDKRDRLTTALHQAFGTRIRFADPAGGMFLWVSVDGIDDAAALLPHAIAEKVLFVPGTGFYAERRARPAFRLSFVGADPAQIEEGVARLARAAARAGVSVVNPD</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="97" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LBS3</accession>
    <name>Q1LBS3_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Aldehyde dehydrogenase</fullName>
            <ecNumber evidence="6">1.2.1.3</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Rmet_5544</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="7">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="7">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the aldehyde dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.2.1.3" evidence="6"/>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12403.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5544"/>
    <dbReference type="EnsemblBacteria" id="ABF12403">
        <property type="protein sequence ID" value="ABF12403"/>
        <property type="gene ID" value="Rmet_5544"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5544"/>
    <dbReference type="eggNOG" id="COG1012">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005391_0_0_4"/>
    <dbReference type="OMA" id="ALRWCGT"/>
    <dbReference type="OrthoDB" id="744602at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004029">
        <property type="term" value="F:aldehyde dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043878">
        <property type="term" value="F:glyceraldehyde-3-phosphate dehydrogenase (NAD+) (non-phosphorylating) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd07106">
        <property type="entry name" value="ALDH_AldA-AAD23400"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.309.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.605.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016161">
        <property type="entry name" value="Ald_DH/histidinol_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016163">
        <property type="entry name" value="Ald_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016160">
        <property type="entry name" value="Ald_DH_CS_CYS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029510">
        <property type="entry name" value="Ald_DH_CS_GLU"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016162">
        <property type="entry name" value="Ald_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015590">
        <property type="entry name" value="Aldehyde_DH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044086">
        <property type="entry name" value="FUS7/LUC3-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00171">
        <property type="entry name" value="Aldedh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53720">
        <property type="entry name" value="SSF53720"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00070">
        <property type="entry name" value="ALDEHYDE_DEHYDR_CYS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00687">
        <property type="entry name" value="ALDEHYDE_DEHYDR_GLU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 4 6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-0614">Plasmid</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aldedh" evidence="5">
        <location>
            <begin position="31"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="active site" evidence="2">
        <location>
            <position position="255"/>
        </location>
    </feature>
    <feature type="active site" evidence="3">
        <location>
            <position position="289"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABF12403.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="486" mass="51354" checksum="C5BB2FB478628CD1" modified="2006-05-30" version="1">MKYLTADAISEALRGNPVRQLINGSMVDGAETLEVINPATGEACAIAPVASLRQLDEAVDAARRSQQSWGGLPLTERRTALKGLATILREHVAELAALLTLEQGRPLAQTEAEVMRAAMLLEAMLTIDIDDEILREDESGRVILQHKPIGVVGAIAPWNVPIGLAVPKITHALYAGNTVVLKPSQYTPLATLRLGEYASNLFPPGVLNVLNGGNDLGERICTHPDIAKISLTGSVPTGKRVMASAAASLKRLTLELGGNDACIVRQDADVDKIAPALFAAAFINSGQVCMAIKRLFVHQDLHERLVEKLGGLAAKAKVGDGFDSTSQMGPVQNRAQYESVKAVLAEVAADPAAIIVAGGEALSRQGFFIAPTVVSGVREGNSLVDKETFGPVLPVLSFQTDEEAIERANAGSMGLGASVWGNDLKMAEHVARQLVAGTVWINRHVGVDPLVPFGGAKESGLGRQFGKAGLLEFTETSALFVPRANK</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-10-07" version="35" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LC05</accession>
    <name>Q1LC05_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_5462</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12321.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF12321">
        <property type="protein sequence ID" value="ABF12321"/>
        <property type="gene ID" value="Rmet_5462"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5462"/>
    <dbReference type="HOGENOM" id="CLU_2094832_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF12321.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="116" mass="12914" checksum="4F05D4FB3D19E450" modified="2006-05-30" version="1">MMPLKPEQFDELRNWPLQLVDIVFVEEWRLIRTPAGRVHLVGRFSPGGRLKASLPLAALNLSSRSVVTLTGKKLRLQGEPGCTAIGKCIASMYGIFDRRMLEAQDVTDAYVSLTAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LCE8</accession>
    <name>Q1LCE8_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Heavy metal cation tricomponent efflux efflux pump ZniA (CzcA-like)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">zniA</name>
        <name evidence="4" type="ordered locus">Rmet_5319</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="5">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="5">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the resistance-nodulation-cell division (RND) (TC 2.A.6) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12178.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519722.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5319"/>
    <dbReference type="TCDB" id="2.A.6.1.17">
        <property type="family name" value="the resistance-nodulation-cell division (rnd) superfamily"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF12178">
        <property type="protein sequence ID" value="ABF12178"/>
        <property type="gene ID" value="Rmet_5319"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5319"/>
    <dbReference type="eggNOG" id="COG3696">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002755_1_2_4"/>
    <dbReference type="OMA" id="QDNVEEY"/>
    <dbReference type="OrthoDB" id="91817at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008324">
        <property type="term" value="F:cation transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2090.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027463">
        <property type="entry name" value="AcrB_DN_DC_subdom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001036">
        <property type="entry name" value="Acrflvin-R"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004763">
        <property type="entry name" value="CzcA/CusA/SilA/NccA/HelA/CnrA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32063">
        <property type="entry name" value="PTHR32063"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00873">
        <property type="entry name" value="ACR_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00702">
        <property type="entry name" value="ACRIFLAVINRP"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82714">
        <property type="entry name" value="SSF82714"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00914">
        <property type="entry name" value="2A0601"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0614">Plasmid</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="336"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="362"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="388"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="456"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="489"/>
            <end position="512"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="544"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="884"/>
            <end position="903"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="910"/>
            <end position="930"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="936"/>
            <end position="961"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="982"/>
            <end position="1001"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="1013"/>
            <end position="1035"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF12178.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="1045" mass="114036" checksum="75718AA6181682C3" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LCL6</accession>
    <name>Q1LCL6_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_5251</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12110.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519656.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5251"/>
    <dbReference type="EnsemblBacteria" id="ABF12110">
        <property type="protein sequence ID" value="ABF12110"/>
        <property type="gene ID" value="Rmet_5251"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5251"/>
    <dbReference type="eggNOG" id="COG1923">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2397493_0_0_4"/>
    <dbReference type="OMA" id="VYKRAIC"/>
    <dbReference type="OrthoDB" id="2075838at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005001">
        <property type="entry name" value="Hfq"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010920">
        <property type="entry name" value="LSM_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17209">
        <property type="entry name" value="Hfq"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50182">
        <property type="entry name" value="SSF50182"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF12110.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="106" mass="11800" checksum="3C0A2A3A81A21988" modified="2006-05-30" version="1">MKKEKKAPRPSRKLQHLHYAAHGASRNAVIVHLSNGTRLTGVVLASDNYMVLLGQSAEDPTPTLIYKRAITVVTPAHAPETTSVLLEAETSPDFVRLYTPRTRKRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="49" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LDA8</accession>
    <name>Q1LDA8_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_5006</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11868.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5006"/>
    <dbReference type="EnsemblBacteria" id="ABF11868">
        <property type="protein sequence ID" value="ABF11868"/>
        <property type="gene ID" value="Rmet_5006"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5006"/>
    <dbReference type="HOGENOM" id="CLU_1785313_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="13"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="93"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="19"/>
            <end position="36"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF11868.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="145" mass="15667" checksum="8265121C325A19BB" modified="2006-05-30" version="1">MRPCIKVRFAVGEGSRRAVPKGASDDSDDGSKEAPLTHGRRTAGARQAHGRRTAGARRTSLLGLDGIQYAEFREGPDASGTPMLDHVKFGVSDESRSAGRWQQGQQCPASALPCEPLRELTSGVRHWPRRAQHRSGLPSTRGQKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LDH3</accession>
    <name>Q1LDH3_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Putative transcriptional regulator, IclR family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Rmet_4941</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="7">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="7">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11803.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4941"/>
    <dbReference type="EnsemblBacteria" id="ABF11803">
        <property type="protein sequence ID" value="ABF11803"/>
        <property type="gene ID" value="Rmet_4941"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4941"/>
    <dbReference type="eggNOG" id="COG1414">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_062618_6_3_4"/>
    <dbReference type="OMA" id="LWQMVQQ"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029016">
        <property type="entry name" value="GAF-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014757">
        <property type="entry name" value="Tscrpt_reg_IclR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005471">
        <property type="entry name" value="Tscrpt_reg_IclR_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09339">
        <property type="entry name" value="HTH_IclR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01614">
        <property type="entry name" value="IclR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00346">
        <property type="entry name" value="HTH_ICLR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51077">
        <property type="entry name" value="HTH_ICLR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51078">
        <property type="entry name" value="ICLR_ED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0614">Plasmid</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH iclR-type" evidence="4">
        <location>
            <begin position="47"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="domain" description="IclR-ED" evidence="5">
        <location>
            <begin position="109"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABF11803.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="301" mass="31619" checksum="E7DAAB1E08AEBE6C" modified="2006-05-30" version="1">MEYLTPSSERPPEGKNRSDSSQPMPKTAYAIPAADAADAAPALKSTSPAVVRAVQVMDAVAAAHEPLTLADLTRLTGAPKSSLHGMCDTLVQLQLLKRLPGGAMALGPHVMSWANAFLSQTQITEEFRALWDETDAFHDATVTLSMLDGAEVVYLACQNGDRPLGVTFRIGMRLPAPYTATGKAMLSTVPAAEVRNLFDGGMPVPLTRASVATVAALVDELEQVRALGYSIDNGQMRDGMTCFGAPVFDATRSRAVGAIAVSYLTSEIDAPTGKQIGGQVRALADQLSARLGAGHLSRALG</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LDY3</accession>
    <name>Q1LDY3_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Putative branched-chain amino acid ABC transport system permease protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Rmet_4781</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="8">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="8">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11643.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519211.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4781"/>
    <dbReference type="EnsemblBacteria" id="ABF11643">
        <property type="protein sequence ID" value="ABF11643"/>
        <property type="gene ID" value="Rmet_4781"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4781"/>
    <dbReference type="eggNOG" id="COG0559">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039929_2_1_4"/>
    <dbReference type="OMA" id="MPEFGEF"/>
    <dbReference type="OrthoDB" id="1841075at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001851">
        <property type="entry name" value="ABC_transp_permease"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02653">
        <property type="entry name" value="BPD_transp_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0614">Plasmid</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="38"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="62"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="97"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="141"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="190"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="225"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="263"/>
            <end position="284"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABF11643.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="288" mass="30128" checksum="EC80BA4C4142FF31" modified="2006-05-30" version="1">MISSLPGVLFDGVAYGSLLFLISIGLSVTMGLMNFVNLAHGAFAMLGGYVAVVLMNRAGVPFLAALPLAFLGAAAAGWVLERTLYRRLYRASHLDQVLFSIGLTFMAMAGATWLFGPGQQPVVLPDFLIGQVRVGGVDLGAYRLFLIAVVIAITLALGWLVARTRFGAQVRAAVDHQQAARGLGIDVERVFSLTFALGSGLAGLGGGLGIDVLGLDPTFPLKYMVYFLLVVAVGGAGSIRGSLVAAIVLGVADVAGKYYVPEIGAFVIYAMMVLLLVAFPAGLYGRRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LE22</accession>
    <name>Q1LE22_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Histone-like DNA-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Rmet_4742</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="5">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="5">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the bacterial histone-like protein family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11604.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4742"/>
    <dbReference type="EnsemblBacteria" id="ABF11604">
        <property type="protein sequence ID" value="ABF11604"/>
        <property type="gene ID" value="Rmet_4742"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4742"/>
    <dbReference type="eggNOG" id="COG0776">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_105066_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030527">
        <property type="term" value="F:structural constituent of chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.520.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000119">
        <property type="entry name" value="Hist_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010992">
        <property type="entry name" value="IHF-like_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33175">
        <property type="entry name" value="PTHR33175"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00216">
        <property type="entry name" value="Bac_DNA_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00411">
        <property type="entry name" value="BHL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47729">
        <property type="entry name" value="SSF47729"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0614">Plasmid</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="82"/>
            <end position="129"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU003939"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF11604.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="230" mass="24695" checksum="660A2E85810C9586" modified="2006-05-30" version="1">MRKWGDVTLRSVFADANQLQNTGFSLGLPCGMYENPFTSGRFRYDSVRTGTDVQQHAKRCDRPAHYLQLSYIDMATKAKPTAKTATKPAAKKAAAPKAPVKAPVKAAAKKAAPAKKAAPAAAAPTPKPLKDTFNKSSLVAHLVAQTQLEAKAVKTVLAHLENTIVSALNKKGAGEFTLPGLLKVTAQQVPAKKKRFGKDPFTGEERWFPAKPASVKVKVRPLKKLKDAAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LE24</accession>
    <name>Q1LE24_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">GCN5-related N-acetyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_4740</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11602.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519174.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4740"/>
    <dbReference type="EnsemblBacteria" id="ABF11602">
        <property type="protein sequence ID" value="ABF11602"/>
        <property type="gene ID" value="Rmet_4740"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4740"/>
    <dbReference type="eggNOG" id="COG3153">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_081840_2_0_4"/>
    <dbReference type="OMA" id="HIMSSKA"/>
    <dbReference type="OrthoDB" id="1687413at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008080">
        <property type="term" value="F:N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="SSF55729"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="1">
        <location>
            <begin position="3"/>
            <end position="148"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF11602.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="168" mass="18034" checksum="206DD247846541BA" modified="2006-05-30" version="1">MTISVRTERPEDVETIAQLTEAAFRGETHSSHTEAFIINGLRRAGCLTLSLVAEDESGIVGHVAASPVTISSGNSGWYGIGPVSVLPDRQRRGIGSVLMLAALDELRRLGARGCVVLGEPAYYGRFGFQADSRLVLPGVPQEYFQTLAFGPDVPVGTVRYHDAFDATE</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="98" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LEF0</accession>
    <name>Q1LEF0_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Short-chain dehydrogenase/reductase SDR</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_4614</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11476.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519051.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4614"/>
    <dbReference type="EnsemblBacteria" id="ABF11476">
        <property type="protein sequence ID" value="ABF11476"/>
        <property type="gene ID" value="Rmet_4614"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4614"/>
    <dbReference type="eggNOG" id="COG1028">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010194_1_1_4"/>
    <dbReference type="OMA" id="GMCADWG"/>
    <dbReference type="OrthoDB" id="1294334at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00061">
        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF11476.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="250" mass="26409" checksum="428CD2D84773A750" modified="2006-05-30" version="1">MKETLLGLDGKRALVTGASSGLGAHFAQRLAAHGAEVVLAARRVDALQSVAKQLEPYGRARCVALDVTSASSRAAMVEEAGPIDILVNNAGLVREGAALKHSEEDWDVVLDTNLKGMFFMAQALASGMRERGGGSIINVASILGLRQAGGVVSYAVSKAGVVQLTRTLALEWARHGIRVNALAPGYIDTEINHDFWQTDAGKALIQRIPQRRLGQPDDLDGPLLLLASDASRYMTGAVLPVDGGHLVNTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LEF7</accession>
    <name>Q1LEF7_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="9">TonB-dependent copper receptor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">oprC</name>
        <name evidence="9" type="ordered locus">Rmet_4607</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="10">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="10">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cell outer membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the TonB-dependent receptor family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11469.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4607"/>
    <dbReference type="EnsemblBacteria" id="ABF11469">
        <property type="protein sequence ID" value="ABF11469"/>
        <property type="gene ID" value="Rmet_4607"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4607"/>
    <dbReference type="eggNOG" id="COG4771">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014873_2_1_4"/>
    <dbReference type="OMA" id="MPDYWEI"/>
    <dbReference type="OrthoDB" id="173242at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009279">
        <property type="term" value="C:cell outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.130.10">
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="BtuB-like"/>
    </dbReference>
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        <property type="entry name" value="Plug_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037066">
        <property type="entry name" value="Plug_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010100">
        <property type="entry name" value="TonB-dep_Cu_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000531">
        <property type="entry name" value="TonB-dep_rcpt_b-brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036942">
        <property type="entry name" value="TonB_rcpt_b-brl_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30069">
        <property type="entry name" value="PTHR30069"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07715">
        <property type="entry name" value="Plug"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00593">
        <property type="entry name" value="TonB_dep_Rec"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01778">
        <property type="entry name" value="TonB-copper"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0998">Cell outer membrane</keyword>
    <keyword evidence="3 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4 9" id="KW-0675">Receptor</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0798">TonB box</keyword>
    <feature type="domain" description="Plug" evidence="8">
        <location>
            <begin position="92"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="domain" description="TonB_dep_Rec" evidence="7">
        <location>
            <begin position="244"/>
            <end position="723"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009810"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003357"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00593"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF07715"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ABF11469.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="724" mass="78068" checksum="90BD68E9D3550462" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LEI6</accession>
    <name>Q1LEI6_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">ABC-type dipeptide/oligopeptide/nickel transport system, substrate binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">dppA</name>
        <name evidence="3" type="ordered locus">Rmet_4578</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11440.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4578"/>
    <dbReference type="EnsemblBacteria" id="ABF11440">
        <property type="protein sequence ID" value="ABF11440"/>
        <property type="gene ID" value="Rmet_4578"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4578"/>
    <dbReference type="eggNOG" id="COG0747">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017028_7_4_4"/>
    <dbReference type="OMA" id="FEFTIIA"/>
    <dbReference type="OrthoDB" id="162819at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043190">
        <property type="term" value="C:ATP-binding cassette (ABC) transporter complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030288">
        <property type="term" value="C:outer membrane-bounded periplasmic space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030678">
        <property type="entry name" value="Peptide/Ni-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039424">
        <property type="entry name" value="SBP_5"/>
    </dbReference>
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        <property type="entry name" value="SBP_5_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30290">
        <property type="entry name" value="PTHR30290"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00496">
        <property type="entry name" value="SBP_bac_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002741">
        <property type="entry name" value="MppA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193390" evidence="1">
        <location>
            <begin position="37"/>
            <end position="575"/>
        </location>
    </feature>
    <feature type="domain" description="SBP_bac_5" evidence="2">
        <location>
            <begin position="109"/>
            <end position="470"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF11440.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="575" mass="65160" checksum="AD040FDD3E4524C0" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LEV4</accession>
    <name>Q1LEV4_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Extra-cytoplasmic solute receptor (Bug)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">bug</name>
        <name evidence="4" type="ordered locus">Rmet_4457</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="5">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="5">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Periplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the UPF0065 (bug) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11322.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518925.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4457"/>
    <dbReference type="EnsemblBacteria" id="ABF11322">
        <property type="protein sequence ID" value="ABF11322"/>
        <property type="gene ID" value="Rmet_4457"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4457"/>
    <dbReference type="eggNOG" id="COG3181">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045683_0_0_4"/>
    <dbReference type="OMA" id="MKQLRYD"/>
    <dbReference type="OrthoDB" id="447734at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042597">
        <property type="term" value="C:periplasmic space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005064">
        <property type="entry name" value="BUG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042100">
        <property type="entry name" value="Bug_dom1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42928">
        <property type="entry name" value="PTHR42928"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03401">
        <property type="entry name" value="TctC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017082">
        <property type="entry name" value="YflP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-0675">Receptor</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193434" evidence="3">
        <location>
            <begin position="29"/>
            <end position="327"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF11322.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="327" mass="34564" checksum="394FC5CDC482A8A5" modified="2006-05-30" version="1">MPNFRIRRTLAAAILALPAAAISLPASANDAYPSRPIRLVVPFAAGSGTDAVARITAKELGDALNQNVIVDNRPGANGSIAAEIVAQAAPDGYTLFMTTNTTHSANPSLMKKLPYDPIKDFTPVARMGNLPFMLVINPKLPVKTVGELIAYAKSHPGMSYASGNSTGIVSGATLARMAQIDLLHVPYKSTPPAMTDVIAGQVPMMFVDVAAGIANVKAGRMRALAVTTAQRSRLLPDLPPIGDAPELKGFDITSWNGVFAPAKTPQPIVDRLNRELSKIAASKEVAPKFEALGFEAFGQTPQQFAGFVGSELVKWNKLVKDAGIQPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="95" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LEY4</accession>
    <name>Q1LEY4_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Acyl-CoA dehydrogenase(Butyryl-CoA dehydrogenase)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Rmet_4427</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="9">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="9">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the acyl-CoA dehydrogenase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11292.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518898.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4427"/>
    <dbReference type="EnsemblBacteria" id="ABF11292">
        <property type="protein sequence ID" value="ABF11292"/>
        <property type="gene ID" value="Rmet_4427"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4427"/>
    <dbReference type="eggNOG" id="COG1960">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018204_3_5_4"/>
    <dbReference type="OMA" id="EIQMRII"/>
    <dbReference type="OrthoDB" id="760677at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016627">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-CH group of donors"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.540.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.110.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006091">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046373">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036250">
        <property type="entry name" value="AcylCo_DH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009075">
        <property type="entry name" value="AcylCo_DH/oxidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013786">
        <property type="entry name" value="AcylCoA_DH/ox_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037069">
        <property type="entry name" value="AcylCoA_DH/ox_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009100">
        <property type="entry name" value="AcylCoA_DH/oxidase_NM_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00441">
        <property type="entry name" value="Acyl-CoA_dh_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02770">
        <property type="entry name" value="Acyl-CoA_dh_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02771">
        <property type="entry name" value="Acyl-CoA_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47203">
        <property type="entry name" value="SSF47203"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56645">
        <property type="entry name" value="SSF56645"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0274">FAD</keyword>
    <keyword evidence="3" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="8" id="KW-0614">Plasmid</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Acyl-CoA_dh_N" evidence="7">
        <location>
            <begin position="5"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA_dh_M" evidence="6">
        <location>
            <begin position="120"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA_dh_1" evidence="5">
        <location>
            <begin position="237"/>
            <end position="383"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF02770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF02771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ABF11292.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="388" mass="42736" checksum="622E1E8E53FCA4C6" modified="2006-05-30" version="1">MQKLTEERRMIQEAARQFTMERVLPIANRLDPEKGQIPRDLIDEMAELGYFGILIPEEYGGLGLGAYEYCLVAEQLSRGWMSVGSLIARGNGLIGALKGLAPEKKAEYLPRMARGEFLGAFSLSEPNAGSDVANISCRAVRDGDDWLITGNKYWCTFADQSDFILVICRTDPVIDPKARHKGLSAFLVEKPRGELPEGVKGSVIPKIGYHGWTTWELAFDNCRVPHSSMVGEEGKAFYLTTAGLETARAHTAARSIGLAQGSLEDSIQYAKDRAQFGAPIGNFQAIRFKLADMATQIEASRALLYTVCEKIDEGVRADTEASMVKLFASEMCERVTSEGLQIHGGAGYTTHFAAERYWRDARLTKIFEGTSEIQMRIISDAMLGKVAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="99" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LF46</accession>
    <name>Q1LF46_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">LysR-type transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">cbbR3</name>
        <name evidence="3" type="ordered locus">Rmet_4365</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the LysR transcriptional regulatory family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11230.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518847.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4365"/>
    <dbReference type="EnsemblBacteria" id="ABF11230">
        <property type="protein sequence ID" value="ABF11230"/>
        <property type="gene ID" value="Rmet_4365"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4365"/>
    <dbReference type="eggNOG" id="COG0583">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039613_6_1_4"/>
    <dbReference type="OMA" id="GATACIH"/>
    <dbReference type="OrthoDB" id="750230at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005119">
        <property type="entry name" value="LysR_subst-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000847">
        <property type="entry name" value="Tscrpt_reg_HTH_LysR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00126">
        <property type="entry name" value="HTH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03466">
        <property type="entry name" value="LysR_substrate"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00039">
        <property type="entry name" value="HTHLYSR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50931">
        <property type="entry name" value="HTH_LYSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH lysR-type" evidence="2">
        <location>
            <begin position="1"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF11230.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="316" mass="35218" checksum="D16F3271FA64071D" modified="2006-05-30" version="1">MKNATLRQLKVFETVARHLSFSRAAEELHLTQPAVSTQVRQLELHVGLPLFEQLGKRIYLTPAGTEMLHYSRCIIQQFREAEDAMAQLKGISGGRLNVAVISAGDYFFPRLLAEFMNRHESVTLNLAVHNREELLHQLAGNLTDLAVMVRPPEGMDTIAEAFAPHPYVIVAAPNHPLVDQRNIPLADLAEEAFVSREKGSDTWNSMQEGFAGRLSNLRIAMEIKSTETIKQAVIANMGIAFLSAHTVGLELQAGKLAVLDIQGFPVMLNWYVVHRKNKRLPPVALAFKHFLMEEGATLIQQITGVEGLIRPRAIHK</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="59" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LF47</accession>
    <name>Q1LF47_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_4364</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11229.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518846.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF11229">
        <property type="protein sequence ID" value="ABF11229"/>
        <property type="gene ID" value="Rmet_4364"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4364"/>
    <dbReference type="eggNOG" id="ENOG5033FNF">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_176089_0_0_4"/>
    <dbReference type="OMA" id="LCGIECE"/>
    <dbReference type="OrthoDB" id="1741226at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.240">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023198">
        <property type="entry name" value="PGP-like_dom2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF11229.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="102" mass="11622" checksum="684D1C4E55A9C401" modified="2006-05-30" version="1">MNQLQPAAPITAPAVSEKGQPDREMQLAAYNAAFAELGLRFRWDMQMFDWLCGIECEKTRVARYIEDFHAHLLKAYEASFLSELIFDKKSEYMRAMAGAGVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="117" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LF53</accession>
    <name>Q1LF53_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Succinate--CoA ligase [ADP-forming] subunit alpha</fullName>
            <ecNumber evidence="2">6.2.1.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">Succinyl-CoA synthetase subunit alpha</fullName>
            <shortName evidence="2">SCS-alpha</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">sucD1</name>
        <name evidence="2" type="synonym">sucD</name>
        <name evidence="7" type="ordered locus">Rmet_4358</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="8">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="8">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2 5">Succinyl-CoA synthetase functions in the citric acid cycle (TCA), coupling the hydrolysis of succinyl-CoA to the synthesis of either ATP or GTP and thus represents the only step of substrate-level phosphorylation in the TCA. The alpha subunit of the enzyme binds the substrates coenzyme A and phosphate, while succinate binding and nucleotide specificity is provided by the beta subunit.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2 5">
            <text>ATP + CoA + succinate = ADP + phosphate + succinyl-CoA</text>
            <dbReference type="Rhea" id="RHEA:17661"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57292"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.2.1.5"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 5">Carbohydrate metabolism; tricarboxylic acid cycle; succinate from succinyl-CoA (ligase route): step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2 5">Heterotetramer of two alpha and two beta subunits.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 4">Belongs to the succinate/malate CoA ligase alpha subunit family.</text>
    </comment>
    <dbReference type="EC" id="6.2.1.5" evidence="2"/>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11223.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518841.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4358"/>
    <dbReference type="EnsemblBacteria" id="ABF11223">
        <property type="protein sequence ID" value="ABF11223"/>
        <property type="gene ID" value="Rmet_4358"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4358"/>
    <dbReference type="eggNOG" id="COG0074">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_052104_0_0_4"/>
    <dbReference type="OMA" id="GEGMKHT"/>
    <dbReference type="OrthoDB" id="1081709at2"/>
    <dbReference type="UniPathway" id="UPA00223">
        <property type="reaction ID" value="UER00999"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004775">
        <property type="term" value="F:succinate-CoA ligase (ADP-forming) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.261">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01988">
        <property type="entry name" value="Succ_CoA_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017440">
        <property type="entry name" value="Cit_synth/succinyl-CoA_lig_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033847">
        <property type="entry name" value="Citrt_syn/SCS-alpha_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003781">
        <property type="entry name" value="CoA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005810">
        <property type="entry name" value="CoA_lig_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005811">
        <property type="entry name" value="CoA_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016102">
        <property type="entry name" value="Succinyl-CoA_synth-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02629">
        <property type="entry name" value="CoA_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00549">
        <property type="entry name" value="Ligase_CoA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001553">
        <property type="entry name" value="SucCS_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00881">
        <property type="entry name" value="CoA_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52210">
        <property type="entry name" value="SSF52210"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01019">
        <property type="entry name" value="sucCoAalpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01216">
        <property type="entry name" value="SUCCINYL_COA_LIG_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00399">
        <property type="entry name" value="SUCCINYL_COA_LIG_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4 7" id="KW-0436">Ligase</keyword>
    <keyword evidence="1 2 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0614">Plasmid</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0816">Tricarboxylic acid cycle</keyword>
    <feature type="domain" description="CoA_binding" evidence="6">
        <location>
            <begin position="4"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="region of interest" description="Coenzyme A binding" evidence="2">
        <location>
            <begin position="17"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Coenzyme A binding" evidence="2">
        <location>
            <begin position="96"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="active site" description="Tele-phosphohistidine intermediate" evidence="2 3">
        <location>
            <position position="248"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="43"/>
        </location>
        <ligand>
            <name>CoA</name>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="159"/>
        </location>
        <ligand>
            <name>substrate</name>
            <note>ligand shared with subunit beta</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01988"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR001553-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU000699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABF11223.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="290" mass="29363" checksum="A089828D4F005E84" modified="2006-05-30" version="1">MAVLIDQHTRVICQGFTGAQGTFHSEQALAYGTKMVGGVTPGKGGETHLGLPVFDTVSAAAHATGADASVIYVPPPFAADAILEAVDAGIGLIVCITEGIPVIDMVRVKRALSGSASRLVGPNCPGVITPGQCKIGIMPGHIHKPGRIGVVSRSGTLTYEAVAQTTATGLGQSTCIGIGGDPVNGTNFIDSLELFLADDQTEGIIMIGEIGGSAEEEAAQFLRDARTRKPVVGFIAGSTAPPGRRMGHAGAIISGGTGTAEAKIEAMREAGIHVAASPAELGNTMAAAMR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LFI7</accession>
    <name>Q1LFI7_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Transcriptional regulator, IclR family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Rmet_4222</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="5">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="5">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11089.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518712.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4222"/>
    <dbReference type="EnsemblBacteria" id="ABF11089">
        <property type="protein sequence ID" value="ABF11089"/>
        <property type="gene ID" value="Rmet_4222"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4222"/>
    <dbReference type="eggNOG" id="COG1414">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_062618_0_0_4"/>
    <dbReference type="OMA" id="DEMIYLE"/>
    <dbReference type="OrthoDB" id="370329at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029016">
        <property type="entry name" value="GAF-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005471">
        <property type="entry name" value="Tscrpt_reg_IclR_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09339">
        <property type="entry name" value="HTH_IclR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00346">
        <property type="entry name" value="HTH_ICLR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51077">
        <property type="entry name" value="HTH_ICLR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0614">Plasmid</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH iclR-type" evidence="3">
        <location>
            <begin position="18"/>
            <end position="80"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF11089.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="266" mass="29058" checksum="27D543C4316695A1" modified="2006-05-30" version="1">MLEPTHESEPFSRTTPGSQTLLRGLNLLRAFVSGAPVLSNAQLAERSGLPRPTVSRLTHSLVEGGYLEYDGVSKGYRLAPVCLSLARSFHIGRSELDAVLPLMAQVAMAEQINVTLSAADGFWMVYLHTIRKGRGLMSRAAMTGTRFGMVRSSTGHAYLAGLPESRRQLLMGRLASHYGEKWPKYKERIDTSRAKYEKDGYCRSRVVLGMEGIATAVMGPDGVLYTLSVSYAVGDDDDGPLEARYAPVLLSLRDAIEIAWSEFGGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="69" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LFT9</accession>
    <name>Q1LFT9_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Heavy metal cation tricomponent efflux outer membrane porin HmyC (CzcC-like)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">hmyC</name>
        <name evidence="3" type="ordered locus">Rmet_4120</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the outer membrane factor (OMF) (TC 1.B.17) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10987.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518615.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4120"/>
    <dbReference type="EnsemblBacteria" id="ABF10987">
        <property type="protein sequence ID" value="ABF10987"/>
        <property type="gene ID" value="Rmet_4120"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4120"/>
    <dbReference type="eggNOG" id="COG1538">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012817_14_0_4"/>
    <dbReference type="OMA" id="SAYRFGE"/>
    <dbReference type="OrthoDB" id="1533572at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015562">
        <property type="term" value="F:efflux transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003423">
        <property type="entry name" value="OMP_efflux"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02321">
        <property type="entry name" value="OEP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193146" evidence="2">
        <location>
            <begin position="23"/>
            <end position="460"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF10987.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="460" mass="49794" checksum="8324894BB6004476" modified="2006-05-30" version="1">MKTKKTLTWAAALSLMSAPAWAAQPVPAGVTAAPGAASPQAAKLAAKAMARATTPAPDTFDLPQLLQLAQSTNKGVEAAQANVDAATAAITSARAYPNPQVEVMYGRLSGKQPGVTSGNAPSYAIVQKLDYPNQRNLREQMAGRGLEASEAMRQGFRSDLAARVKTSYYQVLRRETELAAAREDLNMMRQIQERAKVRVNVGEAPRYELIKADTELLAAQKTLQTAELRVDQAKATLRQQVGGVMPARFALDGSLGHAPDMPPLATLRDTLQASNAELTQRRSELERAKLGVDYQRALRWPEVAVRASTDRQPDNNVSQIGLVMTIPIWDRRSGPVGEATAQATQARTALEAREFELMQELDTAYRQYEIAQAQVTALESGIVREAESALGVAESAYRFGERGILDYLDAQRVLRSARSELIAAQYELQVAAIQIDKLMSASPGVNTAPASSMPDLPNLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="69" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LFX2</accession>
    <name>Q1LFX2_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">ABC_trans_aux domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_4087</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10954.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4087"/>
    <dbReference type="EnsemblBacteria" id="ABF10954">
        <property type="protein sequence ID" value="ABF10954"/>
        <property type="gene ID" value="Rmet_4087"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4087"/>
    <dbReference type="eggNOG" id="COG3009">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_096001_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005586">
        <property type="entry name" value="ABC_trans_aux"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03886">
        <property type="entry name" value="ABC_trans_aux"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC_trans_aux" evidence="1">
        <location>
            <begin position="21"/>
            <end position="179"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF10954.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="188" mass="20032" checksum="7707D2BED4D1E363" modified="2010-03-23" version="2">MASVALIGLLAGCSSPKSRFYTLDPDNTVERASTATPIRVVVGPVTIPDLVDRPQIVTRVSANEVKVNEFARWAEPLKSEISQVIAADLRTMLGSDQVTVFDTGSNAMPAWRVRVDILSLDSEPAVAVSMDALWTIQPPGKQPAIAGRSVVREAVASDGFDAIIAAHNRALASVSRDIATSIRTQPGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LG56</accession>
    <name>Q1LG56_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Probable two-component response regulator with EAL domain, transcription regulator protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Rmet_4002</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="5">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="5">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10870.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518510.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4002"/>
    <dbReference type="EnsemblBacteria" id="ABF10870">
        <property type="protein sequence ID" value="ABF10870"/>
        <property type="gene ID" value="Rmet_4002"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4002"/>
    <dbReference type="eggNOG" id="COG2200">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_70_2_4"/>
    <dbReference type="OMA" id="LILCDMM"/>
    <dbReference type="OrthoDB" id="1635706at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01948">
        <property type="entry name" value="EAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.450">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001633">
        <property type="entry name" value="EAL_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035919">
        <property type="entry name" value="EAL_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00563">
        <property type="entry name" value="EAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00052">
        <property type="entry name" value="EAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF141868">
        <property type="entry name" value="SSF141868"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50883">
        <property type="entry name" value="EAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="4" id="KW-0614">Plasmid</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Response regulatory" evidence="2">
        <location>
            <begin position="9"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="domain" description="EAL" evidence="3">
        <location>
            <begin position="147"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="1">
        <location>
            <position position="59"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF10870.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="404" mass="44010" checksum="22D0D77D871F3C6F" modified="2006-05-30" version="1">MPAQFEALRVLIVEDHPFQRAVAGHLLHRLGAMNTHAAADGREAEQALREQAFDLVLCDIDMPNCNGPELMARLTRDREHAFFGTPPTWAWTSSLAPDILDSHRAFASEHIFPRVYALAKPLNLITLQAILEEVATSKSVLNIANHNGPTDDELRDLTSHPDALVFMLQPQFHIATGRLAGAEALCRWQHPRLGLIPPTLFIPRLEALDAADVIFLTAIDRCLAALILLAKAGVAVPISVNASAQTLCRPGLVERLESVVAASGVPRARLTLELTEETTVRDPFALGIALNRLRLLGYRIAIDDFGIGIATLKLLADLPFTQMKVDKSFVSQMLAGNQRATICRSIIRLAKDLSMECVAEGVETPAQCAALHALGCDIGQGYLWAPPEPIDVFLNAAPGRYGQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LGD9</accession>
    <name>Q1LGD9_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_3919</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10787.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518434.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3919"/>
    <dbReference type="EnsemblBacteria" id="ABF10787">
        <property type="protein sequence ID" value="ABF10787"/>
        <property type="gene ID" value="Rmet_3919"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3919"/>
    <dbReference type="eggNOG" id="COG5361">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027269_1_1_4"/>
    <dbReference type="OMA" id="LYEMARM"/>
    <dbReference type="OrthoDB" id="547506at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.600">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1610">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010621">
        <property type="entry name" value="DUF1214"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037049">
        <property type="entry name" value="DUF1214_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010679">
        <property type="entry name" value="DUF1254"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037050">
        <property type="entry name" value="DUF1254_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06742">
        <property type="entry name" value="DUF1214"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06863">
        <property type="entry name" value="DUF1254"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF1254" evidence="2">
        <location>
            <begin position="54"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="domain" description="DUF1214" evidence="1">
        <location>
            <begin position="308"/>
            <end position="418"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF06742"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF06863"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF10787.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="438" mass="47869" checksum="9472BF81522A8A17" modified="2006-05-30" version="1">MTQQENTAQQRARDAVIHTLPLYEMARMRAATCPRRDHTGRFAGDGPESTLRWVNHVIRPRQLLGPQHRQVVTPNNDTLYTNAWIDLSRGPVVLEVPDFNGRYYVLGLLDFYTNPFGYIGSRTTGTSAGRFLLHGPDWLGTVPAGMRAVACPTNAVWMIGRLLVDGEADLPVVHALQDAIALRQLDGSLAAFAFDVAMQPEEHLGDARRFAEVVNRVVGENPPLGAEAAEIAAFAEVGIGHGIVPTPQQIDLLDAALRGVLADLAEPQPSDMGGGWAMSVDVRESFGSNYLQRALVARNYIGALGVQEAMYVMADRGGDGEPLDGRHAYRLDFAADNLPDVGAFWSLTMYDKGDCMLVPNDISRYSLGDRSPSLTRGADGSLSLYLSATPPRDEALRGNWLPAPPGPFYVALRLYVPQPSHLNRTYRYPAIQLLEVPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LGF4</accession>
    <name>Q1LGF4_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative serine protein kinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">yeaG</name>
        <name evidence="2" type="ordered locus">Rmet_3904</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10772.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008647020.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3904"/>
    <dbReference type="EnsemblBacteria" id="ABF10772">
        <property type="protein sequence ID" value="ABF10772"/>
        <property type="gene ID" value="Rmet_3904"/>
    </dbReference>
    <dbReference type="GeneID" id="60824242"/>
    <dbReference type="KEGG" id="rme:Rmet_3904"/>
    <dbReference type="eggNOG" id="COG2766">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028588_1_0_4"/>
    <dbReference type="OMA" id="DFYGMED"/>
    <dbReference type="OrthoDB" id="49410at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013153">
        <property type="entry name" value="Prk_AAA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010650">
        <property type="entry name" value="PrkA_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016230">
        <property type="entry name" value="Ser_kinase_PrkA"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08298">
        <property type="entry name" value="AAA_PrkA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06798">
        <property type="entry name" value="PrkA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000549">
        <property type="entry name" value="Ser_prot_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00763">
        <property type="entry name" value="AAA_PrkA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0418">Kinase</keyword>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="AAA_PrkA" evidence="1">
        <location>
            <begin position="19"/>
            <end position="376"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF10772.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="640" mass="73388" checksum="EA89865608247673" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="92" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LGG6</accession>
    <name>Q1LGG6_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Putative oxidoreductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_3892</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10760.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518407.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3892"/>
    <dbReference type="EnsemblBacteria" id="ABF10760">
        <property type="protein sequence ID" value="ABF10760"/>
        <property type="gene ID" value="Rmet_3892"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3892"/>
    <dbReference type="eggNOG" id="COG1028">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010194_1_3_4"/>
    <dbReference type="OMA" id="NYQTCYN"/>
    <dbReference type="OrthoDB" id="1294334at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF10760.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="244" mass="25392" checksum="EF317050E35360C1" modified="2006-05-30" version="1">MSNTHPTVIITGATSGIGLGLAKAFLKEGYNVVGTGRSQERLQATAAQLDAGERFVGVAGDVALPDTAREVFAQAIARFGHVDVLVNNAGIFSAKPFVQFTPEEIELQIATNLKGTLYPSQEAARHMSERRQGKIINITASIALQPHAGVPALLAVALKGGLNQATRALALELAPFGVTVNAVAPGVTDTPMHAAETHDALGRLHPLGRIARIDEINAAVLYLAKADFVSGTVLAVDGGYSAGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LGN7</accession>
    <name>Q1LGN7_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Cysteine synthase A</fullName>
            <ecNumber evidence="4">2.5.1.47</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">cysK</name>
        <name evidence="4" type="ordered locus">Rmet_3819</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="5">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="5">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <dbReference type="EC" id="2.5.1.47" evidence="4"/>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10689.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518335.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3819"/>
    <dbReference type="EnsemblBacteria" id="ABF10689">
        <property type="protein sequence ID" value="ABF10689"/>
        <property type="gene ID" value="Rmet_3819"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3819"/>
    <dbReference type="eggNOG" id="COG0031">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021018_1_0_4"/>
    <dbReference type="OMA" id="WMADYGF"/>
    <dbReference type="OrthoDB" id="1677960at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004124">
        <property type="term" value="F:cysteine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006535">
        <property type="term" value="P:cysteine biosynthetic process from serine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1100">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001216">
        <property type="entry name" value="P-phosphate_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001926">
        <property type="entry name" value="PLP-dep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036052">
        <property type="entry name" value="Trypto_synt_PLP_dependent"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00291">
        <property type="entry name" value="PALP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53686">
        <property type="entry name" value="SSF53686"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00901">
        <property type="entry name" value="CYS_SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PALP" evidence="3">
        <location>
            <begin position="10"/>
            <end position="301"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF10689.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="324" mass="34297" checksum="883B92B54ABFDD4B" modified="2006-05-30" version="1">MATRNGFIGAVGNTPLIRLGKLSAETGCEILGKAEFLNPGGSVKDRAALYIIADAERRGTLKPGGTVVEGTAGNTGIGLAHICAARGYRCVIVIPETQSKEKMDLLRVLGAEVRTVPAVPYADPNNYQKVAGRLAQELDNAIWANQFDNTVNRQAHYETTGPEIWRDTDGKIDAFVCATGTGGTLAGVARFLKAQNPAVRTVLADPHGSGLYSFVKTGQIRAEGNSITEGIGSSRITANLADTPIDDAVRIDDQRCVTMVYRLLREEGLYVGGSTGINVAAAVQIAQDLGPGHTIVTLLCDRGSLYSARLFNAEWLAQKGLVAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="95" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LGW5</accession>
    <name>Q1LGW5_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 7">Flagellar basal-body rod protein FlgG</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6 7">Distal rod protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="primary">flgG</name>
        <name evidence="10" type="ordered locus">Rmet_3739</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="11">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="11">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="5 7">The basal body constitutes a major portion of the flagellar organelle and consists of four rings (L,P,S, and M) mounted on a central rod. The rod consists of about 26 subunits of FlgG in the distal portion, and FlgB, FlgC and FlgF are thought to build up the proximal portion of the rod with about 6 subunits each.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Bacterial flagellum basal body</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the flagella basal body rod proteins family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10611.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008641159.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3739"/>
    <dbReference type="EnsemblBacteria" id="ABF10611">
        <property type="protein sequence ID" value="ABF10611"/>
        <property type="gene ID" value="Rmet_3739"/>
    </dbReference>
    <dbReference type="GeneID" id="60826293"/>
    <dbReference type="KEGG" id="rme:Rmet_3739"/>
    <dbReference type="eggNOG" id="COG4786">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013687_0_1_4"/>
    <dbReference type="OMA" id="TRDGTFH"/>
    <dbReference type="OrthoDB" id="1488472at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009426">
        <property type="term" value="C:bacterial-type flagellum basal body, distal rod"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071973">
        <property type="term" value="P:bacterial-type flagellum-dependent cell motility"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001444">
        <property type="entry name" value="Flag_bb_rod_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019776">
        <property type="entry name" value="Flagellar_basal_body_rod_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020013">
        <property type="entry name" value="Flagellar_FlgE/F/G"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010930">
        <property type="entry name" value="Flg_bb/hook_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037925">
        <property type="entry name" value="FlgE/F/G-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012834">
        <property type="entry name" value="FlgG_G_neg"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00460">
        <property type="entry name" value="Flg_bb_rod"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06429">
        <property type="entry name" value="Flg_bbr_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117143">
        <property type="entry name" value="SSF117143"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03506">
        <property type="entry name" value="FlgEFG_subfam"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02488">
        <property type="entry name" value="flgG_G_neg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00588">
        <property type="entry name" value="FLAGELLA_BB_ROD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0975">Bacterial flagellum</keyword>
    <keyword evidence="10" id="KW-0966">Cell projection</keyword>
    <keyword evidence="10" id="KW-0969">Cilium</keyword>
    <keyword evidence="10" id="KW-0282">Flagellum</keyword>
    <keyword evidence="10" id="KW-0614">Plasmid</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Flg_bb_rod" evidence="8">
        <location>
            <begin position="8"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="domain" description="Flg_bbr_C" evidence="9">
        <location>
            <begin position="183"/>
            <end position="260"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00017948"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023143"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00025933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00032912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU362116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF06429"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ABF10611.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="261" mass="27890" checksum="6F38F7F97EC1C1C4" modified="2006-05-30" version="1">MIRSLWIAKTGLDAQQTQMDVISNNLANVSTTGFKRSRAVFEEMMYQTIRQPGAMSSDQTVLPSGMQIGTGVRPVATERIHTQGNPQQTGNAKDVAIIGNGFFQVTMPDGTTSYTRDGSFQVDQNGQLVTSSGFVINPAITLPANYTSVNIARDGTVTVMQPGSTNAATVGQLQLATFPNPTGLESMGENLYVQTDASGAPNTTVPGLNGAGTLQNNYVEASNVNVVEELVSMIQTQRAYEINSKAVQTSDQMLQRLTQMG</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="50" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LHD3</accession>
    <name>Q1LHD3_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative Cu(II)/Cu(I) resistance protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">copQ1</name>
        <name evidence="2" type="ordered locus">Rmet_3571</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10443.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF10443">
        <property type="protein sequence ID" value="ABF10443"/>
        <property type="gene ID" value="Rmet_3571"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3571"/>
    <dbReference type="HOGENOM" id="CLU_136209_5_0_4"/>
    <dbReference type="OMA" id="HAGSIDP"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193172" evidence="1">
        <location>
            <begin position="31"/>
            <end position="90"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF10443.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="90" mass="9598" checksum="C3F5D36C330AAC74" modified="2006-05-30" version="1">MNVKEDSIMKRQFALIAALTVALSAAPAFAKAGKFDTYTDGARASKFDPYTDGAKSGKFDTYTDGAKSGKFDTYTDGAKSFDVYSDGAKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="59" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LHE7</accession>
    <name>Q1LHE7_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_3557</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10429.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517973.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3557"/>
    <dbReference type="EnsemblBacteria" id="ABF10429">
        <property type="protein sequence ID" value="ABF10429"/>
        <property type="gene ID" value="Rmet_3557"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3557"/>
    <dbReference type="eggNOG" id="ENOG503334Q">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_183045_4_0_4"/>
    <dbReference type="OMA" id="WIFAWFV"/>
    <dbReference type="OrthoDB" id="2071349at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021741">
        <property type="entry name" value="DUF3311"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11755">
        <property type="entry name" value="DUF3311"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="5"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="35"/>
            <end position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF10429.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="69" mass="7914" checksum="C3D8B4FBCF20DCA8" modified="2006-05-30" version="1">MLKLLIGIGIPYVGVLGLLPWVASVDTFVLGVPFVYAWMFAWFVLTSVCLYVCWRFFDRPVDAERRLDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LHI6</accession>
    <name>Q1LHI6_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">ABC-type transporter, periplasmic component</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_3518</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10390.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517940.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3518"/>
    <dbReference type="EnsemblBacteria" id="ABF10390">
        <property type="protein sequence ID" value="ABF10390"/>
        <property type="gene ID" value="Rmet_3518"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3518"/>
    <dbReference type="eggNOG" id="COG0683">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027128_1_0_4"/>
    <dbReference type="OMA" id="DIQAADY"/>
    <dbReference type="OrthoDB" id="776281at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028081">
        <property type="entry name" value="Leu-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028082">
        <property type="entry name" value="Peripla_BP_I"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13458">
        <property type="entry name" value="Peripla_BP_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53822">
        <property type="entry name" value="SSF53822"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193129" evidence="1">
        <location>
            <begin position="25"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="domain" description="Peripla_BP_6" evidence="2">
        <location>
            <begin position="30"/>
            <end position="365"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF10390.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="402" mass="43195" checksum="E72887117D0C8E82" modified="2006-05-30" version="1">MKMTRLAVAVAATVLGAFAGGASAQVSGDTVKIGYITDMSGLYADIDGPGGLEAVKMAIEDHGGKVLGKPIELVSADHQNKADIAASKAREWMDQQGLDMLLGGTNSATGLAMNKVAQEKKRVYFNVGAGTARLTNEECSPYTVHYAYDTVALAKGTGSAVVKQGGKKWFFLTADYAFGHSLENDTAAVVKANGGTVVGSVRHPLSASDFSSFLLQAQSSKADILGLANAGGDTINAIKAAKEFGITKNMKIAGLLMFINDIHSLGLKNTEGLLMTDSWYWDMNDDTRKFANRFFGKMKKMPSSLQAADYSAISTYLKAVEAAKTDDPDKVMAELKKMKISDFYNKSYIRADGRNIHDMYLMQVKAPADSKKPWDYLKIVTTIPGDQAFTTPAESKCALLKK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-12-12" modified="2022-05-25" version="97" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LHL1</accession>
    <name>ATPE_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">ATP synthase epsilon chain</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">ATP synthase F1 sector epsilon subunit</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">F-ATPase epsilon subunit</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">atpC</name>
        <name type="ordered locus">Rmet_3493</name>
    </gene>
    <organism>
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Produces ATP from ADP in the presence of a proton gradient across the membrane.</text>
    </comment>
    <comment type="subunit">
        <text>F-type ATPases have 2 components, CF(1) - the catalytic core - and CF(0) - the membrane proton channel. CF(1) has five subunits: alpha(3), beta(3), gamma(1), delta(1), epsilon(1). CF(0) has three main subunits: a, b and c.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell inner membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the ATPase epsilon chain family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10365.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517921.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q1LHL1"/>
    <dbReference type="SMR" id="Q1LHL1"/>
    <dbReference type="STRING" id="266264.Rmet_3493"/>
    <dbReference type="EnsemblBacteria" id="ABF10365">
        <property type="protein sequence ID" value="ABF10365"/>
        <property type="gene ID" value="Rmet_3493"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3493"/>
    <dbReference type="eggNOG" id="COG0355">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_084338_2_0_4"/>
    <dbReference type="OMA" id="MGGFAEI"/>
    <dbReference type="OrthoDB" id="1696893at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045261">
        <property type="term" value="C:proton-transporting ATP synthase complex, catalytic core F(1)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046933">
        <property type="term" value="F:proton-transporting ATP synthase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd12152">
        <property type="entry name" value="F1-ATPase_delta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.15.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00530">
        <property type="entry name" value="ATP_synth_epsil_bac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036794">
        <property type="entry name" value="ATP_F1_dsu/esu_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001469">
        <property type="entry name" value="ATP_synth_F1_dsu/esu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020547">
        <property type="entry name" value="ATP_synth_F1_dsu/esu_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020546">
        <property type="entry name" value="ATP_synth_F1_dsu/esu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036771">
        <property type="entry name" value="ATPsynth_dsu/esu_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13822">
        <property type="entry name" value="PTHR13822"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00401">
        <property type="entry name" value="ATP-synt_DE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02823">
        <property type="entry name" value="ATP-synt_DE_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46604">
        <property type="entry name" value="SSF46604"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51344">
        <property type="entry name" value="SSF51344"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01216">
        <property type="entry name" value="ATP_synt_epsi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0066">ATP synthesis</keyword>
    <keyword id="KW-0997">Cell inner membrane</keyword>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0139">CF(1)</keyword>
    <keyword id="KW-0375">Hydrogen ion transport</keyword>
    <keyword id="KW-0406">Ion transport</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0813">Transport</keyword>
    <feature type="chain" id="PRO_0000265869" description="ATP synthase epsilon chain">
        <location>
            <begin position="1"/>
            <end position="138"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00530"/>
        </source>
    </evidence>
    <sequence length="138" mass="14530" checksum="C4E0C0DCE274976D" modified="2006-05-30" version="1">MATILVDVVSAEASIFSGQAKFVALPGESGELGILPGHTPLITRIQPGAVRIEKEDGGEEFVFVAGGILEVQPQHVTVLADTAIRGTDLDEAKASEAKRAAEEMLQNQSSDLDLARAQSELAVAAAQLAAIARLRRKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LHX1</accession>
    <name>Q1LHX1_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Putative transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_3383</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10255.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008647898.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3383"/>
    <dbReference type="EnsemblBacteria" id="ABF10255">
        <property type="protein sequence ID" value="ABF10255"/>
        <property type="gene ID" value="Rmet_3383"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3383"/>
    <dbReference type="eggNOG" id="COG1959">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_107144_2_0_4"/>
    <dbReference type="OMA" id="VLMYLTQ"/>
    <dbReference type="OrthoDB" id="1765806at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000944">
        <property type="entry name" value="Tscrpt_reg_Rrf2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33221">
        <property type="entry name" value="PTHR33221"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02082">
        <property type="entry name" value="Rrf2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00738">
        <property type="entry name" value="rrf2_super"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51197">
        <property type="entry name" value="HTH_RRF2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF10255.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="155" mass="16882" checksum="BEAC59CB20A78335" modified="2006-05-30" version="1">MIPVTMQLTRFSDYALRLLMYVSRGDGSRPITIAEVGQQFDISHNHLVKVAARLSKLGWISATRGRHGGLQLGPGAEKLSVGAILRELEGRKAIIDCADPPCALNGNCRLKRALDDAEEAFYRALDGVTLADVSGSRTAESIISLHRDFLSRHVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LHY3</accession>
    <name>Q1LHY3_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Putative multicomponent oxygenase/reductase subunit for phenylacetic acid degradation</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">paaC</name>
        <name evidence="1" type="ordered locus">Rmet_3371</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10243.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517808.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3371"/>
    <dbReference type="EnsemblBacteria" id="ABF10243">
        <property type="protein sequence ID" value="ABF10243"/>
        <property type="gene ID" value="Rmet_3371"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3371"/>
    <dbReference type="eggNOG" id="COG3396">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_070585_0_0_4"/>
    <dbReference type="OMA" id="NGDFAHT"/>
    <dbReference type="OrthoDB" id="1389523at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010124">
        <property type="term" value="P:phenylacetate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1260.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012347">
        <property type="entry name" value="Ferritin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009078">
        <property type="entry name" value="Ferritin-like_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007814">
        <property type="entry name" value="PaaA_PaaC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011882">
        <property type="entry name" value="PaaC"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05138">
        <property type="entry name" value="PaaA_PaaC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037834">
        <property type="entry name" value="PA_CoA_Oase3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47240">
        <property type="entry name" value="SSF47240"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02158">
        <property type="entry name" value="PA_CoA_Oxy3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF10243.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="285" mass="31486" checksum="419CA88195337136" modified="2006-05-30" version="1">MNSSITAASNGDASLAGLPLTRSAPLQYVLRLADNALILGQRNAEWCGHGPALEEDIALTNISLDLIGQARLLYSHAGVLEGQLTGRTRHEDDYAYWRGEREFHNWTLLELPHHGPLAGTARADRDYAITIMRNFLFGALMVELWPALERSRDAELAAIAAKSIKEARYHLHHAAGWVVRLGDGTETSHLRMQRALEHLMPYMNECFAVDPIEREAAEQGIGVLTADLRDAWDATVRATLEEATLRLPTESAFLSTGKQGVHSEHMGYLLAELQGLARAHPGAQW</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LI33</accession>
    <name>Q1LI33_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Dienelactone hydrolase (Carboxymethylenebutenolidase)</fullName>
            <ecNumber evidence="3">3.1.1.45</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_3321</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EC" id="3.1.1.45" evidence="3"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10193.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517772.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3321"/>
    <dbReference type="ESTHER" id="ralme-q1li33">
        <property type="family name" value="Dienelactone_hydrolase"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF10193">
        <property type="protein sequence ID" value="ABF10193"/>
        <property type="gene ID" value="Rmet_3321"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3321"/>
    <dbReference type="eggNOG" id="COG0412">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032662_0_0_4"/>
    <dbReference type="OMA" id="FCFTHTT"/>
    <dbReference type="OrthoDB" id="1889569at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008806">
        <property type="term" value="F:carboxymethylenebutenolidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002925">
        <property type="entry name" value="Dienelactn_hydro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032710">
        <property type="entry name" value="NTF2-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037401">
        <property type="entry name" value="SnoaL-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01738">
        <property type="entry name" value="DLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12680">
        <property type="entry name" value="SnoaL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="SSF53474"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54427">
        <property type="entry name" value="SSF54427"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DLH" evidence="1">
        <location>
            <begin position="14"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="domain" description="SnoaL-like" evidence="2">
        <location>
            <begin position="247"/>
            <end position="354"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12680"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF10193.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="409" mass="45025" checksum="AD27E4FDD4A7B7F8" modified="2006-05-30" version="1">MGQTIQIQTQDGNFSGYLATPAGGKGPGIVLCQEIFGVNATMRQVADYYAEEGYTVLVPDLFWRIEHGIELSDRGADFQRALGLYQQFDEDKGVQDVGAALATLRQRPECAGQTGVLGFCLGGKLAYLAACRLPDVACAVGYYGVGIERALGEARNIRGRLVLHVAERDGFCPPEAQASIREALSGQPGVELYVYPGMDHAFARTGGEHFDKASALMAHQRSIAAFRAEMGPHYDFSALWDKHCEYEFATRDVDATMATMVAEPYVNHIPTMTGGVGYRQLKRFYQHHFVHSNPPDTTLIPLSRTIGASQVVDEILFCFTHTCEIDWMLPGVPPTGKRVEIPLIAIIKFRGDKLYHEHIYWDQASVLVQVGLLDPKGLPVAGVETARKLLDETLPSNTLMARWSESEGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="95" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LIC1</accession>
    <name>Q1LIC1_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Periplasmic serine endoprotease DegP-like</fullName>
            <ecNumber evidence="4">3.4.21.107</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Rmet_3233</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>Acts on substrates that are at least partially unfolded. The cleavage site P1 residue is normally between a pair of hydrophobic residues, such as Val-|-Val.</text>
            <dbReference type="EC" id="3.4.21.107"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Periplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the peptidase S1C family.</text>
    </comment>
    <dbReference type="EC" id="3.4.21.107" evidence="4"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10105.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517715.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3233"/>
    <dbReference type="MEROPS" id="S01.477"/>
    <dbReference type="EnsemblBacteria" id="ABF10105">
        <property type="protein sequence ID" value="ABF10105"/>
        <property type="gene ID" value="Rmet_3233"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3233"/>
    <dbReference type="eggNOG" id="COG0265">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020120_1_2_4"/>
    <dbReference type="OMA" id="IIGINRQ"/>
    <dbReference type="OrthoDB" id="741829at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042597">
        <property type="term" value="C:periplasmic space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001478">
        <property type="entry name" value="PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011782">
        <property type="entry name" value="Pept_S1C_Do"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001940">
        <property type="entry name" value="Peptidase_S1C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13180">
        <property type="entry name" value="PDZ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00834">
        <property type="entry name" value="PROTEASES2C"/>
    </dbReference>
    <dbReference type="SMART" id="SM00228">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="SSF50156"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="SSF50494"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02037">
        <property type="entry name" value="degP_htrA_DO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50106">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 7" id="KW-0645">Protease</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0720">Serine protease</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0346">Stress response</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="domain" description="PDZ" evidence="6">
        <location>
            <begin position="307"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="3">
        <location>
            <position position="133"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="3">
        <location>
            <position position="163"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="3">
        <location>
            <position position="237"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR611782-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU364067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABF10105.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="398" mass="41867" checksum="CC1E9F88166C85E1" modified="2006-05-30" version="1">MLRRFWLFFAQAVTVVLAVWFVVATLKPEWLQRGRVAVQSGSPIVALKEVVPSVEGSAAPGSYSEAARLAMPAVVNIFTSKNGSKRSPNNPQAEDPWFRFFFGDRLPERQEPVSSLGSGVIVSAEGYILTNHHVVDGADEIEVALTDGRKANAKVVGSDPETDLAVLKVTLKDLPAITLGRIENVKVGDVVLAIGNPFGVGQTVTMGIVSALGRSHLGINTFENFIQTDAAINPGNSGGALVDAQGNLLGINTAIYSRSGGSLGIGFAIPVSTAKQVMESIISTGSVTRGWIGVEPQDLTPEIAESFGLEAKEGALIAAVVQGGPADKAGVKPGDVLVSVDNQSISDTTALLNAIAQLKPGAEVKMKVIRRGKPAELTVTIGKRPPPPRRPMPLDEEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="52" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LIG0</accession>
    <name>Q1LIG0_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_3194</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10066.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517675.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3194"/>
    <dbReference type="EnsemblBacteria" id="ABF10066">
        <property type="protein sequence ID" value="ABF10066"/>
        <property type="gene ID" value="Rmet_3194"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3194"/>
    <dbReference type="eggNOG" id="COG3409">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2919463_0_0_4"/>
    <dbReference type="OrthoDB" id="1120636at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF10066.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="61" mass="6620" checksum="2715EAEB73377409" modified="2006-05-30" version="1">MILLHVWRGVPAVRFSRTPEGKATVAAFMDAYNNMVVSLRNYKAQDVKGGLGRGGQLQVGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="115" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LIN1</accession>
    <name>Q1LIN1_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 5">Cell division protein FtsZ</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4 8" type="primary">ftsZ</name>
        <name evidence="8" type="ordered locus">Rmet_3123</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4 5">Essential cell division protein that forms a contractile ring structure (Z ring) at the future cell division site. The regulation of the ring assembly controls the timing and the location of cell division. One of the functions of the FtsZ ring is to recruit other cell division proteins to the septum to produce a new cell wall between the dividing cells. Binds GTP and shows GTPase activity.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homodimer. Polymerizes to form a dynamic ring structure in a strictly GTP-dependent manner. Interacts directly with several other division proteins.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
        <text evidence="4">Assembles at midcell at the inner surface of the cytoplasmic membrane.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4 5">Belongs to the FtsZ family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09995.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008650317.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3123"/>
    <dbReference type="EnsemblBacteria" id="ABF09995">
        <property type="protein sequence ID" value="ABF09995"/>
        <property type="gene ID" value="Rmet_3123"/>
    </dbReference>
    <dbReference type="GeneID" id="60820501"/>
    <dbReference type="KEGG" id="rme:Rmet_3123"/>
    <dbReference type="eggNOG" id="COG0206">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024865_0_1_4"/>
    <dbReference type="OMA" id="GNPSIGQ"/>
    <dbReference type="OrthoDB" id="1009041at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032153">
        <property type="term" value="C:cell division site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000917">
        <property type="term" value="P:division septum assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043093">
        <property type="term" value="P:FtsZ-dependent cytokinesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051258">
        <property type="term" value="P:protein polymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02201">
        <property type="entry name" value="FtsZ_type1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1440">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00909">
        <property type="entry name" value="FtsZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000158">
        <property type="entry name" value="Cell_div_FtsZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020805">
        <property type="entry name" value="Cell_div_FtsZ_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045061">
        <property type="entry name" value="FtsZ/CetZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024757">
        <property type="entry name" value="FtsZ_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008280">
        <property type="entry name" value="Tub_FtsZ_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037103">
        <property type="entry name" value="Tubulin/FtsZ-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018316">
        <property type="entry name" value="Tubulin/FtsZ_2-layer-sand-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036525">
        <property type="entry name" value="Tubulin/FtsZ_GTPase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003008">
        <property type="entry name" value="Tubulin_FtsZ_GTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30314">
        <property type="entry name" value="PTHR30314"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12327">
        <property type="entry name" value="FtsZ_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00091">
        <property type="entry name" value="Tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00423">
        <property type="entry name" value="CELLDVISFTSZ"/>
    </dbReference>
    <dbReference type="SMART" id="SM00864">
        <property type="entry name" value="Tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00865">
        <property type="entry name" value="Tubulin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52490">
        <property type="entry name" value="SSF52490"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55307">
        <property type="entry name" value="SSF55307"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00065">
        <property type="entry name" value="ftsZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01135">
        <property type="entry name" value="FTSZ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="4 5 8" id="KW-0132">Cell division</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 4 5" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="2 4 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5" id="KW-0717">Septation</keyword>
    <feature type="domain" description="Tubulin" evidence="6">
        <location>
            <begin position="15"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="domain" description="Tubulin_C" evidence="7">
        <location>
            <begin position="206"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="23"/>
            <end position="27"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="107"/>
            <end position="109"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="138"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="142"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="186"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU000631"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ABF09995.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="396" mass="41332" checksum="C6F8C5A8F1290C68" modified="2006-05-30" version="1">MDFDMIETEIQDGTIIKVVGVGGAGGNAVQHMISRGVQGVEFICMNTDAQALKRSTASRVLQLGNTGLGAGAKPEVGRNCAEQARDQIADALRGAHMVFITAGMGGGTGTGAAPIVAQVAKEMGILTVGVVSKPFDFEGARRAKVAEHGSGELESSVDSLIVVLNEKLFEVMGDDAEMDKCFQCADDVLHNAVAGIAEIINVDGLVNVDFEDVKTVMGEQGKAMMGTATVSGVDRARLAAEQAVASPLLEGVDLSGARGVLVNITASRSLKLSETKEVMNTIRSYAAEDATVIFGTVYDDSMSDALRVTVVATGLGRSAKKQQPMTLLKTGTDNIPVQMMAGLTAAPAHASPDYSGLDTPAVWRSSRESASAHVAALQEKGVDTYDIPAFLRKQAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LJD8</accession>
    <name>Q1LJD8_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Leucine/isoleucine/valine transporter subunit, membrane component of ABC superfamily</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">livM2</name>
        <name evidence="7" type="ordered locus">Rmet_2865</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09738.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008644903.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2865"/>
    <dbReference type="EnsemblBacteria" id="ABF09738">
        <property type="protein sequence ID" value="ABF09738"/>
        <property type="gene ID" value="Rmet_2865"/>
    </dbReference>
    <dbReference type="GeneID" id="60820696"/>
    <dbReference type="KEGG" id="rme:Rmet_2865"/>
    <dbReference type="eggNOG" id="COG4177">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031365_1_1_4"/>
    <dbReference type="OMA" id="PIFANKY"/>
    <dbReference type="OrthoDB" id="1456257at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015658">
        <property type="term" value="F:branched-chain amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06581">
        <property type="entry name" value="TM_PBP1_LivM_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001851">
        <property type="entry name" value="ABC_transp_permease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043428">
        <property type="entry name" value="LivM-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30482">
        <property type="entry name" value="PTHR30482"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02653">
        <property type="entry name" value="BPD_transp_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="21"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="53"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="78"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="116"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="142"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="204"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="255"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="290"/>
            <end position="315"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABF09738.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="384" mass="41543" checksum="1DCDBFFAD0FBE8D6" modified="2006-05-30" version="1">MNTPIPSTAVAAPSRVPAKTLAALVGFLVVALCAPFFVQTFGGNYWVRVLDFALLYIMLALGLNIVVGFAGLLDLGYIAFYAVGAYMMALLGSPHLSNQFEWIHQLFPNGLHLSMWFVLPIAVVVAATFGVLLGAPTLKLRGDYLAIVTLGFGEIVRIFMNNLDRPVNITNGPKGITAVDPVHIFGFDFSKSHEIFGLKFTPVFMYYYLLVALVIVIVFICLRLQNSRIGRAFVAIREDEIAAKAMGINTRNIKLLAFAMGASFGGASGATFGAFQGFVSPESFTLWESIYVLAIVVLGGMGHIPGVILGGILLVGFQELLRAVAEPIQTGIFGQVIVDAEVLRQLLFGLALVGVMLYRPAGLWPSPRKEDRPVVRRAGSVGRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="55" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LJG3</accession>
    <name>Q1LJG3_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Auxiliry mobilization protein C</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">mobC</name>
        <name evidence="1" type="ordered locus">Rmet_2840</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09713.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517401.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2840"/>
    <dbReference type="EnsemblBacteria" id="ABF09713">
        <property type="protein sequence ID" value="ABF09713"/>
        <property type="gene ID" value="Rmet_2840"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2840"/>
    <dbReference type="eggNOG" id="ENOG5032XJ5">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2094829_0_0_4"/>
    <dbReference type="OMA" id="LAWHANR"/>
    <dbReference type="OrthoDB" id="1601217at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF09713.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="116" mass="12724" checksum="30083A9721EC67FB" modified="2006-05-30" version="1">MTKMHDDGLRKVLAIRLTKGERALVEQKAVEAGLPPATYIREAALRAIVAPVKSDIPEINRAAWASLARVCANLNQYQAAINAGQATGYPAQFLEELRGQVQMLRRAVIGVRDNDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LJK6</accession>
    <name>Q1LJK6_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Malonyl-CoA decarboxylase</fullName>
            <ecNumber evidence="4">4.1.1.9</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">matA</name>
        <name evidence="4" type="ordered locus">Rmet_2797</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="journal article" date="2013" name="Structure" volume="21" first="1182" last="1192">
            <title>Crystal structures of malonyl-coenzyme A decarboxylase provide insights into its catalytic mechanism and disease-causing mutations.</title>
            <authorList>
                <person name="Froese D.S."/>
                <person name="Forouhar F."/>
                <person name="Tran T.H."/>
                <person name="Vollmar M."/>
                <person name="Kim Y.S."/>
                <person name="Lew S."/>
                <person name="Neely H."/>
                <person name="Seetharaman J."/>
                <person name="Shen Y."/>
                <person name="Xiao R."/>
                <person name="Acton T.B."/>
                <person name="Everett J.K."/>
                <person name="Cannone G."/>
                <person name="Puranik S."/>
                <person name="Savitsky P."/>
                <person name="Krojer T."/>
                <person name="Pilka E.S."/>
                <person name="Kiyani W."/>
                <person name="Lee W.H."/>
                <person name="Marsden B.D."/>
                <person name="von Delft F."/>
                <person name="Allerston C.K."/>
                <person name="Spagnolo L."/>
                <person name="Gileadi O."/>
                <person name="Montelione G.T."/>
                <person name="Oppermann U."/>
                <person name="Yue W.W."/>
                <person name="Tong L."/>
            </authorList>
            <dbReference type="PubMed" id="23791943"/>
            <dbReference type="DOI" id="10.1016/j.str.2013.05.001"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (2.30 ANGSTROMS) OF 57-473</scope>
    </reference>
    <dbReference type="EC" id="4.1.1.9" evidence="4"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09670.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517365.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="PDB" id="4KS9">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.30 A"/>
        <property type="chains" value="A/B=57-473"/>
    </dbReference>
    <dbReference type="PDBsum" id="4KS9"/>
    <dbReference type="SMR" id="Q1LJK6"/>
    <dbReference type="DIP" id="DIP-60408N"/>
    <dbReference type="STRING" id="266264.Rmet_2797"/>
    <dbReference type="EnsemblBacteria" id="ABF09670">
        <property type="protein sequence ID" value="ABF09670"/>
        <property type="gene ID" value="Rmet_2797"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2797"/>
    <dbReference type="eggNOG" id="COG1593">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023433_3_1_4"/>
    <dbReference type="OMA" id="QPITWDS"/>
    <dbReference type="OrthoDB" id="864913at2"/>
    <dbReference type="BRENDA" id="4.1.1.9">
        <property type="organism ID" value="5272"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050080">
        <property type="term" value="F:malonyl-CoA decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006633">
        <property type="term" value="P:fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038917">
        <property type="entry name" value="Malonyl_CoA_deC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007956">
        <property type="entry name" value="Malonyl_CoA_deC_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042303">
        <property type="entry name" value="Malonyl_CoA_deC_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035372">
        <property type="entry name" value="MCD_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038351">
        <property type="entry name" value="MCD_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28641">
        <property type="entry name" value="PTHR28641"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05292">
        <property type="entry name" value="MCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17408">
        <property type="entry name" value="MCD_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword evidence="6" id="KW-0002">3D-structure</keyword>
    <keyword evidence="4" id="KW-0456">Lyase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MCD_N" evidence="3">
        <location>
            <begin position="110"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="domain" description="MCD" evidence="2">
        <location>
            <begin position="194"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="38"/>
            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF05292"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF17408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF09670.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="6">
        <source>
            <dbReference type="PDB" id="4KS9"/>
        </source>
    </evidence>
    <sequence length="473" mass="52592" checksum="B4A2158D02293C92" modified="2006-05-30" version="1">MTALDTSDQQKVSAPLDAAEPVSDTLLSRFGRWWNRKGDADATAAGPAKAQDTPALSTRATKRQRDQLRQCFDARLTDVAANAAAQAWQDEYEAAVEPLRQAMLGVLAEVAAVRDAAGGQPATASGLSQALSNARIRFFKRFAALHGQRGNSACGLHFLIQLRADMLRWHKRIPGLRELDEDLEALFSNWFDVGLLELQPITWDSPASLLEKLIRYEAVHEISSWTDLRNRLDSDRRCYAFFHPRIPREPLIFVEVAFVPEMAANVQALLDEAAPLEDLRRVKWAIFYSISNTQAGLRGVSFGNFLLKRVIEELQREHPKLKQFATLSPIPGFADWLRKRDGESIDRVLGVKRLARWREQHGEVPADGAAWFSALSADTEDTVIRDTAMTLAAHYLVREGGKGVPADPVARFHLGNGACVERVNWGADMSRKGRAQSCGMMVNYLYVPDALDDNLARLGDGNPRISRAVAKLL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-10-31" modified="2022-05-25" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LJX8</accession>
    <name>TOLB_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Tol-Pal system protein TolB</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">tolB</name>
        <name type="ordered locus">Rmet_2675</name>
    </gene>
    <organism>
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Part of the Tol-Pal system, which plays a role in outer membrane invagination during cell division and is important for maintaining outer membrane integrity.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">The Tol-Pal system is composed of five core proteins: the inner membrane proteins TolA, TolQ and TolR, the periplasmic protein TolB and the outer membrane protein Pal. They form a network linking the inner and outer membranes and the peptidoglycan layer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Periplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the TolB family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09548.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517249.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q1LJX8"/>
    <dbReference type="SMR" id="Q1LJX8"/>
    <dbReference type="STRING" id="266264.Rmet_2675"/>
    <dbReference type="PRIDE" id="Q1LJX8"/>
    <dbReference type="EnsemblBacteria" id="ABF09548">
        <property type="protein sequence ID" value="ABF09548"/>
        <property type="gene ID" value="Rmet_2675"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2675"/>
    <dbReference type="eggNOG" id="COG0823">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047123_0_0_4"/>
    <dbReference type="OMA" id="VREPSWG"/>
    <dbReference type="OrthoDB" id="821953at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042597">
        <property type="term" value="C:periplasmic space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007049">
        <property type="term" value="P:cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017038">
        <property type="term" value="P:protein import"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00671">
        <property type="entry name" value="TolB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011042">
        <property type="entry name" value="6-blade_b-propeller_TolB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011659">
        <property type="entry name" value="PD40"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014167">
        <property type="entry name" value="Tol-Pal_TolB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007195">
        <property type="entry name" value="TolB_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07676">
        <property type="entry name" value="PD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04052">
        <property type="entry name" value="TolB_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02800">
        <property type="entry name" value="propeller_TolB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0131">Cell cycle</keyword>
    <keyword id="KW-0132">Cell division</keyword>
    <keyword id="KW-0574">Periplasm</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000259075" description="Tol-Pal system protein TolB" evidence="1">
        <location>
            <begin position="44"/>
            <end position="446"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00671"/>
        </source>
    </evidence>
    <sequence length="446" mass="48010" checksum="4E5221DEC0D1C58E" modified="2006-05-30" version="1" precursor="true">MRKLWAPNWLSRRQNANPTRDQSRHALMAWLAAALMSAGAAHAQLNVEITGVGSNQFPVATANFQGEAQAPQNLTAIIRSDLTNSGRFRNVDPAGATVAESAQVDLGSWKAKGADAFVAGSVTPTSNGQYEVRFRLYDTAKGQSLGGLAFTVTQGQLRVTAHKIADYIYEKLLGERGVFATRLSYVSKVGNRYQLLISDSDGQNAQIALTSTEPIISPAWSPDGRRVAYVSFEAKKPVVYVHDLATGKRVVVSNQKGNNSAPSWSPDGQHLAVSLSRDGNTQIYQVNADGSGLRRLTRSSAIDTEPQFSPDGRSIYFTSDRGGAPQIYRMPASGEESGAAQRVTFKGSYNVSPRISPDGKYLAYISRSGGFRLQLQDLSNGDVTSLTDTTNDESPSFAANGKYILYATRVGGRSVLAAVSTDGRTKQVLSLQSGAVREPSWGPFMQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="103" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LK20</accession>
    <name>Q1LK20_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DNA helicase</fullName>
            <ecNumber evidence="3">3.6.4.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">uvrD</name>
        <name evidence="9" type="ordered locus">Rmet_2629</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + H2O = ADP + H(+) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.12"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the helicase family. UvrD subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.12" evidence="3"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09506.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517205.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2629"/>
    <dbReference type="EnsemblBacteria" id="ABF09506">
        <property type="protein sequence ID" value="ABF09506"/>
        <property type="gene ID" value="Rmet_2629"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2629"/>
    <dbReference type="eggNOG" id="COG0210">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004585_5_2_4"/>
    <dbReference type="OMA" id="YQDTNRT"/>
    <dbReference type="OrthoDB" id="137860at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003678">
        <property type="term" value="F:DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.160">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013986">
        <property type="entry name" value="DExx_box_DNA_helicase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014017">
        <property type="entry name" value="DNA_helicase_UvrD-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000212">
        <property type="entry name" value="DNA_helicase_UvrD/REP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014016">
        <property type="entry name" value="UvrD-like_ATP-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11070">
        <property type="entry name" value="PTHR11070"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00580">
        <property type="entry name" value="UvrD-helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13361">
        <property type="entry name" value="UvrD_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51198">
        <property type="entry name" value="UVRD_HELICASE_ATP_BIND"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51217">
        <property type="entry name" value="UVRD_HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 9" id="KW-0347">Helicase</keyword>
    <keyword evidence="6 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UvrD-like helicase ATP-binding" evidence="7">
        <location>
            <begin position="6"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="domain" description="UvrD-like helicase C-terminal" evidence="8">
        <location>
            <begin position="288"/>
            <end position="592"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="27"/>
            <end position="34"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001665"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ABF09506.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="787" mass="87790" checksum="A07959AF1DF6B560" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="118" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LKK8</accession>
    <name>Q1LKK8_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 12">GTP 3',8-cyclase</fullName>
            <ecNumber evidence="3 12">4.1.99.22</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Molybdenum cofactor biosynthesis protein A</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12 14" type="primary">moaA</name>
        <name evidence="14" type="ordered locus">Rmet_2441</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Catalyzes the cyclization of GTP to (8S)-3',8-cyclo-7,8-dihydroguanosine 5'-triphosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 12">
            <text>AH2 + GTP + S-adenosyl-L-methionine = (8S)-3',8-cyclo-7,8-dihydroguanosine 5'-triphosphate + 5'-deoxyadenosine + A + H(+) + L-methionine</text>
            <dbReference type="Rhea" id="RHEA:49576"/>
            <dbReference type="ChEBI" id="CHEBI:13193"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17319"/>
            <dbReference type="ChEBI" id="CHEBI:17499"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:131766"/>
            <dbReference type="EC" id="4.1.99.22"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="12">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="12">Binds 2 [4Fe-4S] clusters. Binds 1 [4Fe-4S] cluster coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine and 1 [4Fe-4S] cluster coordinated with 3 cysteines and the GTP-derived substrate.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 12">Cofactor biosynthesis; molybdopterin biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="12">Monomer and homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the radical SAM superfamily. MoaA family.</text>
    </comment>
    <dbReference type="EC" id="4.1.99.22" evidence="3 12"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09318.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2441"/>
    <dbReference type="EnsemblBacteria" id="ABF09318">
        <property type="protein sequence ID" value="ABF09318"/>
        <property type="gene ID" value="Rmet_2441"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2441"/>
    <dbReference type="eggNOG" id="COG2896">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009273_0_1_4"/>
    <dbReference type="OMA" id="IEFMPIG"/>
    <dbReference type="UniPathway" id="UPA00344"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019008">
        <property type="term" value="C:molybdopterin synthase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061798">
        <property type="term" value="F:GTP 3',8'-cyclase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904047">
        <property type="term" value="F:S-adenosyl-L-methionine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006777">
        <property type="term" value="P:Mo-molybdopterin cofactor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01225_B">
        <property type="entry name" value="MoaA_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaB/NifB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013483">
        <property type="entry name" value="MoaA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000385">
        <property type="entry name" value="MoaA_NifB_PqqE_Fe-S-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010505">
        <property type="entry name" value="Mob_synth_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06463">
        <property type="entry name" value="Mob_synth_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02666">
        <property type="entry name" value="moaA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01305">
        <property type="entry name" value="MOAA_NIFB_PQQE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="9 12" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="7 12" id="KW-0408">Iron</keyword>
    <keyword evidence="8 12" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="11 12" id="KW-0456">Lyase</keyword>
    <keyword evidence="5 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10 12" id="KW-0501">Molybdenum cofactor biosynthesis</keyword>
    <keyword evidence="6 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 12" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <feature type="domain" description="Radical SAM core" evidence="13">
        <location>
            <begin position="58"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="67"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="74"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="78"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="80"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="81"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="121"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="125"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="156"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="180"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="218"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="252"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="316"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
            <note>4Fe-4S-substrate</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="319"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
            <note>4Fe-4S-substrate</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="321"/>
            <end position="323"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="333"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
            <note>4Fe-4S-substrate</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005046"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ABF09318.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="395" mass="44002" checksum="07D7F9CEEDC499EF" modified="2006-05-30" version="1">MADRKASLAPARRRASVLTLAMTDTVIPILDLRDHRYRSMVPSIPSHLVAPNGLVADTRGRPLHDLRISVTDRCNFRCVYCMPKEVFDKDYTFLPHSELLSFEEIERTARIFVSQGVEKIRLTGGEPLLRKNIERLVEMLARIETVSGKPLDLTLTTNASLLARKAQSLRDAGLSRISVSLDAIDDAIFRKMNDVDYAVRDVLHGIEVAQAVGLAPIKVNMVVKRGTNDGEIVPLARHFRGSGIIMRFIEFMDVGASNHWQMGEVLPSAEVVRRIDEVFPLDAVAANYSGETAERWRYRDGGGEIGVISSVTQAFCGDCSRIRLSTEGRLYLCLFATEGYDLRALLRGGHSDLEIANAIAQVWQGRTDNYSEQRSDPAIRAARAERKIEMSYIGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="106" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LKZ3</accession>
    <name>Q1LKZ3_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Lipoprotein signal peptidase</fullName>
            <ecNumber evidence="6">3.4.23.36</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Prolipoprotein signal peptidase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">Signal peptidase II</fullName>
            <shortName evidence="6">SPase II</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">pbrC2</name>
        <name evidence="6" type="synonym">lspA</name>
        <name evidence="9" type="ordered locus">Rmet_2304</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6 7">This protein specifically catalyzes the removal of signal peptides from prolipoproteins.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6 7">
            <text>Release of signal peptides from bacterial membrane prolipoproteins. Hydrolyzes -Xaa-Yaa-Zaa-|-(S,diacylglyceryl)Cys-, in which Xaa is hydrophobic (preferably Leu), and Yaa (Ala or Ser) and Zaa (Gly or Ala) have small, neutral side chains.</text>
            <dbReference type="EC" id="3.4.23.36"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="6">Protein modification; lipoprotein biosynthesis (signal peptide cleavage).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cell inner membrane</location>
            <topology evidence="6">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 6 8">Belongs to the peptidase A8 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="3.4.23.36" evidence="6"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09183.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_003098955.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2304"/>
    <dbReference type="EnsemblBacteria" id="ABF09183">
        <property type="protein sequence ID" value="ABF09183"/>
        <property type="gene ID" value="Rmet_2304"/>
    </dbReference>
    <dbReference type="GeneID" id="56409812"/>
    <dbReference type="GeneID" id="61526430"/>
    <dbReference type="KEGG" id="rme:Rmet_2304"/>
    <dbReference type="eggNOG" id="COG0597">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_083252_4_0_4"/>
    <dbReference type="OMA" id="GAPWWSL"/>
    <dbReference type="OrthoDB" id="1575081at2"/>
    <dbReference type="UniPathway" id="UPA00665"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004190">
        <property type="term" value="F:aspartic-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00161">
        <property type="entry name" value="LspA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001872">
        <property type="entry name" value="Peptidase_A8"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33695">
        <property type="entry name" value="PTHR33695"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01252">
        <property type="entry name" value="Peptidase_A8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00781">
        <property type="entry name" value="LIPOSIGPTASE"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00077">
        <property type="entry name" value="lspA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00855">
        <property type="entry name" value="SPASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6 7" id="KW-0064">Aspartyl protease</keyword>
    <keyword evidence="6" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="2 6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4 6 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 7" id="KW-0645">Protease</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="69"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="active site" evidence="6">
        <location>
            <position position="120"/>
        </location>
    </feature>
    <feature type="active site" evidence="6">
        <location>
            <position position="138"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU004181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ABF09183.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="166" mass="18157" checksum="941F028174124A00" modified="2006-05-30" version="1">MDEPSLRKTQWYSLAIVIFAGDQAAKSYIDATTPLGWSHEVASFFNLVHALNPGAAFSFLAGAGGWQRWFFLAIAFAISAWLAWLLSRPLRKTEGLSYSLILGGALGNAFDRATRGHVIDYIDFHLHGWHWPAFNIADMAIVGGAIALVAQSFMSVENPAATKESQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-10-07" version="62" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LL10</accession>
    <name>Q1LL10_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF305 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_2287</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09166.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2287"/>
    <dbReference type="EnsemblBacteria" id="ABF09166">
        <property type="protein sequence ID" value="ABF09166"/>
        <property type="gene ID" value="Rmet_2287"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2287"/>
    <dbReference type="eggNOG" id="COG3544">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_113754_1_1_4"/>
    <dbReference type="OMA" id="MEWLIED"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1260.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005183">
        <property type="entry name" value="DUF305"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012347">
        <property type="entry name" value="Ferritin-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03713">
        <property type="entry name" value="DUF305"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="42"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="72"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="domain" description="DUF305" evidence="2">
        <location>
            <begin position="95"/>
            <end position="146"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF03713"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF09166.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="151" mass="16997" checksum="C09C40064B1D8D41" modified="2006-05-30" version="1">MGKHGRPYAKFWVNMVLGLVVMYFVMFSMIDGARDFRNNLNMLYMAVTMWAPMGIFMLATMPGMFPNRRLNLVLYGLFAVLTLGSFAATRAQTGIGDRQFIASMVPHHSGAILMCREAQLSDPELVNLCQAISDGQRAEIEQMNRIAARLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-10-07" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LL21</accession>
    <name>Q1LL21_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_2276</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09155.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2276"/>
    <dbReference type="EnsemblBacteria" id="ABF09155">
        <property type="protein sequence ID" value="ABF09155"/>
        <property type="gene ID" value="Rmet_2276"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2276"/>
    <dbReference type="HOGENOM" id="CLU_2587191_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="52"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF09155.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="80" mass="9183" checksum="8CE1BD602F8018F4" modified="2006-05-30" version="1">MPTDAARIRGWKESRSRIAQCATNKNHYRLYCHPDTLPKSGAPPGNRFLRGIYFWSVIIMELLLSLLVGCGISLILFYRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LLI1</accession>
    <name>Q1LLI1_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Metallopeptidase, M23B subfamily</fullName>
            <ecNumber evidence="3">3.4.24.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_2116</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EC" id="3.4.24.-" evidence="3"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08995.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516829.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2116"/>
    <dbReference type="EnsemblBacteria" id="ABF08995">
        <property type="protein sequence ID" value="ABF08995"/>
        <property type="gene ID" value="Rmet_2116"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2116"/>
    <dbReference type="eggNOG" id="COG1388">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG4942">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029425_0_3_4"/>
    <dbReference type="OMA" id="WTWPANG"/>
    <dbReference type="OrthoDB" id="1891666at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00118">
        <property type="entry name" value="LysM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.70.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.350.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011055">
        <property type="entry name" value="Dup_hybrid_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018392">
        <property type="entry name" value="LysM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036779">
        <property type="entry name" value="LysM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016047">
        <property type="entry name" value="Peptidase_M23"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01476">
        <property type="entry name" value="LysM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01551">
        <property type="entry name" value="Peptidase_M23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00257">
        <property type="entry name" value="LysM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51261">
        <property type="entry name" value="SSF51261"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51782">
        <property type="entry name" value="LYSM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="LysM" evidence="2">
        <location>
            <begin position="59"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="39"/>
            <end position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF08995.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="295" mass="30528" checksum="632CB4648A7E0FEF" modified="2006-05-30" version="1">MHNIVKSDSFARVGQRLSVVAIAAVLAACANSPMPAPVVDRTSTAGAQQAASTEPAPPGYYRVKRGDTLYRIALENGQSYRDVASWNNMANANQIEVGQLLRVVPPGADVNAAPGVATAPVASGVVQSQPIDSSRPVGAPVGATTSTPAAVAAAPASAAAAPAATPSASASAPASDIRLAWPASGQLIGKFDEKSNKGIDIAGKKGDAVLAADEGRVIHVGPLRGYGNLVIIKHNDTYLTAYGHNDKVLVTEQSTVRKGQKIAEMGNSDSDRVKLHFEVRKNGKPVDPMRYLPPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LLI4</accession>
    <name>Q1LLI4_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Metal-dependent hydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_2113</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08992.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516826.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2113"/>
    <dbReference type="EnsemblBacteria" id="ABF08992">
        <property type="protein sequence ID" value="ABF08992"/>
        <property type="gene ID" value="Rmet_2113"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2113"/>
    <dbReference type="eggNOG" id="COG3568">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_064248_0_0_4"/>
    <dbReference type="OMA" id="IQWGRGA"/>
    <dbReference type="OrthoDB" id="1387978at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005135">
        <property type="entry name" value="Endo/exonuclease/phosphatase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03372">
        <property type="entry name" value="Exo_endo_phos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="SSF56219"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Endo/exonuclease/phosphatase" evidence="2">
        <location>
            <begin position="5"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="165"/>
            <end position="184"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF03372"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF08992.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="284" mass="31777" checksum="0735906A9706EFA4" modified="2006-05-30" version="1">MELISWNIQWGRGADGKVDLARIVDTLRGMADADVLCLQEITRGFSDLAGHPGPDQVAELSALLPDYQVIFAPGVDRTHRDGSPRQFGNVIATRLPVREIFRHALPWPADPDVASMPRVALEVTVQAGSRLLRIICTHLEYYSTSQRAAQTEALRDWHVQACDHARHPGRSENRPGPFTPEPRPSEAILCGDFNSRPEDGAYLRMVETFGGVTPDWHDAWIHMHPGQPHAPTCALFDKEQWPEPPFACDFVFVTDDLLGNVRRCEVNDRTDASDHQPIHLTTDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="102" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LLL9</accession>
    <name>Q1LLL9_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 6">Carbonic anhydrase</fullName>
            <ecNumber evidence="3 6">4.2.1.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Carbonate dehydratase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">cynT</name>
        <name evidence="8" type="ordered locus">Rmet_2078</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Reversible hydration of carbon dioxide.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 6">
            <text>H(+) + hydrogencarbonate = CO2 + H2O</text>
            <dbReference type="Rhea" id="RHEA:10748"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <dbReference type="EC" id="4.2.1.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the beta-class carbonic anhydrase family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.1" evidence="3 6"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08957.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516791.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2078"/>
    <dbReference type="EnsemblBacteria" id="ABF08957">
        <property type="protein sequence ID" value="ABF08957"/>
        <property type="gene ID" value="Rmet_2078"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2078"/>
    <dbReference type="eggNOG" id="COG0288">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_053879_5_3_4"/>
    <dbReference type="OMA" id="EFIERWV"/>
    <dbReference type="OrthoDB" id="1841447at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004089">
        <property type="term" value="F:carbonate dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015976">
        <property type="term" value="P:carbon utilization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00884">
        <property type="entry name" value="beta_CA_cladeB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1050.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045066">
        <property type="entry name" value="Beta_CA_cladeB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001765">
        <property type="entry name" value="Carbonic_anhydrase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015892">
        <property type="entry name" value="Carbonic_anhydrase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036874">
        <property type="entry name" value="Carbonic_anhydrase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11002">
        <property type="entry name" value="PTHR11002"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00484">
        <property type="entry name" value="Pro_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00947">
        <property type="entry name" value="Pro_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53056">
        <property type="entry name" value="SSF53056"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00704">
        <property type="entry name" value="PROK_CO2_ANHYDRASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00705">
        <property type="entry name" value="PROK_CO2_ANHYDRASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6 8" id="KW-0456">Lyase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0862">Zinc</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="220"/>
            <end position="244"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012925"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003956"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ABF08957.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="244" mass="26856" checksum="CFC2F1F8E41EB1E2" modified="2006-05-30" version="1">MHEIERLLKGFERFQRRYFEARPELFDALRTGQKPQTLLIGCSDSRVDPGLLLDCDPGELFTVRNVANLVPPCGDGASGRLHGVSAAIQFAVEQLHVVRIIVMGHAGCGGIRALLAHPQNDDTNAPQDFIGPWVRIAGPARRMVEQTLPNASPAERQRACEQAAILVSLRNLESFPFVREACERGALTLHGWYFDLQAGALLAYSERADDFLPLVCPLTPEARPHRQPSNRPSIDSGQAEMLGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="122" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LLN8</accession>
    <name>Q1LLN8_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 9">DNA-binding transcriptional regulator NtrC</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Nitrogen regulation protein NR(I)</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="primary">glnG</name>
        <name evidence="9" type="synonym">ntrC</name>
        <name evidence="13" type="ordered locus">Rmet_2059</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Member of the two-component regulatory system NtrB/NtrC, which controls expression of the nitrogen-regulated (ntr) genes in response to nitrogen limitation. Phosphorylated NtrC binds directly to DNA and stimulates the formation of open promoter-sigma54-RNA polymerase complexes.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08938.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2059"/>
    <dbReference type="EnsemblBacteria" id="ABF08938">
        <property type="protein sequence ID" value="ABF08938"/>
        <property type="gene ID" value="Rmet_2059"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2059"/>
    <dbReference type="eggNOG" id="COG2204">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_0_1_4"/>
    <dbReference type="OMA" id="DWTGNIR"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000156">
        <property type="term" value="F:phosphorelay response regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009399">
        <property type="term" value="P:nitrogen fixation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006808">
        <property type="term" value="P:regulation of nitrogen utilization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002197">
        <property type="entry name" value="HTH_Fis"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002078">
        <property type="entry name" value="Sigma_54_int"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025662">
        <property type="entry name" value="Sigma_54_int_dom_ATP-bd_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025943">
        <property type="entry name" value="Sigma_54_int_dom_ATP-bd_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025944">
        <property type="entry name" value="Sigma_54_int_dom_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010114">
        <property type="entry name" value="Transcript_reg_NtrC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003903">
        <property type="entry name" value="UIM_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02954">
        <property type="entry name" value="HTH_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00158">
        <property type="entry name" value="Sigma54_activat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01590">
        <property type="entry name" value="HTHFIS"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01818">
        <property type="entry name" value="ntrC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00675">
        <property type="entry name" value="SIGMA54_INTERACT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00676">
        <property type="entry name" value="SIGMA54_INTERACT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00688">
        <property type="entry name" value="SIGMA54_INTERACT_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50045">
        <property type="entry name" value="SIGMA54_INTERACT_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50330">
        <property type="entry name" value="UIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-0010">Activator</keyword>
    <keyword evidence="4 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="9" id="KW-0535">Nitrogen fixation</keyword>
    <keyword evidence="3 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0678">Repressor</keyword>
    <keyword evidence="7 9" id="KW-0804">Transcription</keyword>
    <keyword evidence="5 9" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="9" id="KW-0902">Two-component regulatory system</keyword>
    <feature type="domain" description="Response regulatory" evidence="12">
        <location>
            <begin position="12"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="domain" description="Sigma-54 factor interaction" evidence="11">
        <location>
            <begin position="145"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="397"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="8">
        <location>
            <position position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00019059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU365013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50045"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ABF08938.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="528" mass="58080" checksum="002AF2FC74825FD2" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LLT6</accession>
    <name>Q1LLT6_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_2011</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08890.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516725.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2011"/>
    <dbReference type="EnsemblBacteria" id="ABF08890">
        <property type="protein sequence ID" value="ABF08890"/>
        <property type="gene ID" value="Rmet_2011"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2011"/>
    <dbReference type="HOGENOM" id="CLU_1659312_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="111"/>
            <end position="159"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF08890.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="159" mass="17741" checksum="7DC694D13A4576E6" modified="2006-05-30" version="1">MTARLPHVFPGTPAITTLLRQGHAWMDRLAALEGWSAVLLADLHDRLRRQPAATVADTVEHFRHRLNDAEERERERLRAVRPSVGWCPGCHGECVGTHRSCMPGYGVAPPSPIPEVPQRVEPQPIASPTRPRYQRKPRGLARHLGHVTAPGCKENIEEP</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LLW9</accession>
    <name>Q1LLW9_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Primosomal replication protein N</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1 2" type="primary">priB</name>
        <name evidence="2" type="ordered locus">Rmet_1978</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Binds single-stranded DNA at the primosome assembly site (PAS).</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Component of the preprimosomal complex composed of PriA, PriB, PriC, DnaB and DnaT. Upon transient interaction with DnaG it forms the primosome.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the PriB family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08857.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516699.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1978"/>
    <dbReference type="EnsemblBacteria" id="ABF08857">
        <property type="protein sequence ID" value="ABF08857"/>
        <property type="gene ID" value="Rmet_1978"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1978"/>
    <dbReference type="eggNOG" id="COG2965">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_166075_1_2_4"/>
    <dbReference type="OMA" id="LLDCEGF"/>
    <dbReference type="OrthoDB" id="1942216at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990077">
        <property type="term" value="C:primosome complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006269">
        <property type="term" value="P:DNA replication, synthesis of RNA primer"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00720">
        <property type="entry name" value="PriB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023646">
        <property type="entry name" value="Prisomal_replication_PriB"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF003135">
        <property type="entry name" value="Primosomal_n"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR04418">
        <property type="entry name" value="PriB_gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0235">DNA replication</keyword>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="1" id="KW-0639">Primosome</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00720"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF08857.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="99" mass="11122" checksum="A0BD5EEBD6F44364" modified="2006-05-30" version="1">MNQLRLTATLVEREALRYTPAGVPVVNCILQHAGETLEAETPRQVEFAIVAMGIGAVGQQLERFPLDTLLDCEGFLARKHRNSRTLVFHITRCKAFEKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LLY4</accession>
    <name>Q1LLY4_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Molybdopterin synthase, small subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">moaD</name>
        <name evidence="1" type="ordered locus">Rmet_1963</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08842.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516687.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1963"/>
    <dbReference type="EnsemblBacteria" id="ABF08842">
        <property type="protein sequence ID" value="ABF08842"/>
        <property type="gene ID" value="Rmet_1963"/>
    </dbReference>
    <dbReference type="GeneID" id="60821431"/>
    <dbReference type="KEGG" id="rme:Rmet_1963"/>
    <dbReference type="eggNOG" id="COG1977">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_114601_4_0_4"/>
    <dbReference type="OMA" id="DRVHAKP"/>
    <dbReference type="OrthoDB" id="1995573at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012675">
        <property type="entry name" value="Beta-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016155">
        <property type="entry name" value="Mopterin_synth/thiamin_S_b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003749">
        <property type="entry name" value="ThiS/MoaD-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02597">
        <property type="entry name" value="ThiS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54285">
        <property type="entry name" value="SSF54285"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF08842.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="85" mass="9124" checksum="DD8EA1758B571F62" modified="2006-05-30" version="1">MQLELRYFASVREQVGQGAERAEVPAEVRTVGDLRAWLRGRGGAWTEALADGRALRMAVDHAVARADTPLADGCEVAFFPPVTGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="117" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LMJ5</accession>
    <name>Q1LMJ5_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Sensor protein</fullName>
            <ecNumber evidence="5">2.7.13.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">agrS</name>
        <name evidence="8" type="ordered locus">Rmet_1752</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Member of a two-component regulatory system.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.</text>
            <dbReference type="EC" id="2.7.13.3"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cell inner membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.7.13.3" evidence="5"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08631.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516483.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1752"/>
    <dbReference type="EnsemblBacteria" id="ABF08631">
        <property type="protein sequence ID" value="ABF08631"/>
        <property type="gene ID" value="Rmet_1752"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1752"/>
    <dbReference type="eggNOG" id="COG2205">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_89_6_4"/>
    <dbReference type="OMA" id="CANAIKY"/>
    <dbReference type="OrthoDB" id="692375at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00082">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006290">
        <property type="entry name" value="CztS_silS_copS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003660">
        <property type="entry name" value="HAMP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003661">
        <property type="entry name" value="HisK_dim/P"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036097">
        <property type="entry name" value="HisK_dim/P_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004358">
        <property type="entry name" value="Sig_transdc_His_kin-like_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00672">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00512">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00344">
        <property type="entry name" value="BCTRLSENSOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00304">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00388">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47384">
        <property type="entry name" value="SSF47384"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="SSF55874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01386">
        <property type="entry name" value="cztS_silS_copS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50885">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4 5 8" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5 8" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0902">Two-component regulatory system</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="167"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="domain" description="HAMP" evidence="7">
        <location>
            <begin position="189"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="6">
        <location>
            <begin position="250"/>
            <end position="464"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU364088"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ABF08631.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="469" mass="50960" checksum="4B3B6A931D13C289" modified="2006-05-30" version="1">MRKRSLTARLALAFALIAGLTFSGVGIYLYGSLASQIIDRDDAELLRKAARAQQELAELRSSPTENRWEEVLGVVKGNEEFGIRILDARGVQLAAAGANYGIKPIGGGAYAKVPDAAGSIQAWSTPSDMPVRMTTIKASVSGEASPVVVELYQVASSRVSLLRDYRWKLFIAVLLGTVGAGGLGYAALRAGMAPLRSIAAGTASVTFTSGALPVDPSRLPAELDDLARALQRMIDRLRERYDRLSQFSADLAHDFRTPITNLLGQTQVALSSDRSIEEYQSLLVSNVEEYERLSRMIENMLFLARADNARVAIRATELDLREELHRQADYFESLAEARQITIQVNAQGKVFADPTLFRRALSNLLSNAIRYANEGTSVTVHGRHTGGVAQVKVENVGPGIAPEHLSLLFDRFFRADPARANSGESSGIGLAIVKTIMDLHHGAVSVRSVLNATTSFQLEFPDNRTIPHT</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="70" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LMS4</accession>
    <name>Q1LMS4_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Poly(3-hydroxybutyrate) polymerase</fullName>
            <ecNumber evidence="6">2.3.1.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">phaC2</name>
        <name evidence="6" type="ordered locus">Rmet_1671</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.3.1.-" evidence="6"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08552.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="SMR" id="Q1LMS4"/>
    <dbReference type="STRING" id="266264.Rmet_1671"/>
    <dbReference type="ESTHER" id="ralme-q1lms4">
        <property type="family name" value="PHA_synth_I"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF08552">
        <property type="protein sequence ID" value="ABF08552"/>
        <property type="gene ID" value="Rmet_1671"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1671"/>
    <dbReference type="eggNOG" id="COG3243">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017387_3_0_4"/>
    <dbReference type="OMA" id="FLTWYLR"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016746">
        <property type="term" value="F:acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042619">
        <property type="term" value="P:poly-hydroxybutyrate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010963">
        <property type="entry name" value="PHA_synth_I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010941">
        <property type="entry name" value="PhaC_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07167">
        <property type="entry name" value="PhaC_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="SSF53474"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01838">
        <property type="entry name" value="PHA_synth_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PhaC_N" evidence="5">
        <location>
            <begin position="87"/>
            <end position="253"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF07167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABF08552.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="572" mass="63689" checksum="030E00409386D639" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LMT2</accession>
    <name>Q1LMT2_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Pili assembly chaperone, PapD family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">papD</name>
        <name evidence="9" type="ordered locus">Rmet_1663</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Periplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the periplasmic pilus chaperone family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08544.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516392.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1663"/>
    <dbReference type="EnsemblBacteria" id="ABF08544">
        <property type="protein sequence ID" value="ABF08544"/>
        <property type="gene ID" value="Rmet_1663"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1663"/>
    <dbReference type="eggNOG" id="COG3121">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_070768_0_2_4"/>
    <dbReference type="OMA" id="VNINDYP"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030288">
        <property type="term" value="C:outer membrane-bounded periplasmic space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061077">
        <property type="term" value="P:chaperone-mediated protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008962">
        <property type="entry name" value="PapD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036316">
        <property type="entry name" value="Pili_assmbl_chap_C_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001829">
        <property type="entry name" value="Pili_assmbl_chaperone_bac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016148">
        <property type="entry name" value="Pili_assmbl_chaperone_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018046">
        <property type="entry name" value="Pili_assmbl_chaperone_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016147">
        <property type="entry name" value="Pili_assmbl_chaperone_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02753">
        <property type="entry name" value="PapD_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00345">
        <property type="entry name" value="PapD_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00969">
        <property type="entry name" value="CHAPERONPILI"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49354">
        <property type="entry name" value="SSF49354"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49584">
        <property type="entry name" value="SSF49584"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00635">
        <property type="entry name" value="PILI_CHAPERONE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0143">Chaperone</keyword>
    <keyword evidence="4" id="KW-0574">Periplasm</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="domain" description="PapD_N" evidence="7">
        <location>
            <begin position="53"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="domain" description="PapD_C" evidence="8">
        <location>
            <begin position="202"/>
            <end position="263"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007399"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022764"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ABF08544.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="275" mass="30896" checksum="86D5692057363C41" modified="2006-05-30" version="1">MSPIGRDEPKIRATRIKRIWNDWRFPSGFHALRFVIIFGVMIMQAWNPQAIAGVTPEVSRVVYAADAAEQSLQVFNVNDYPVLAQAWIDDGRIDALPQESTAPIVVIPPIFRLGRGDQTSLRLINSGAALPSDRESLFWLNIYEIPSTPNGSMAESQLVTVTMRTQIKVFVRPRKLPFSSDEIPKRLAFSVKIMTDESLLEIRNPTPYYVTFSKIQTDIGRISQVSSVEMIAPYSQTIAHLGEVKGIGNSARIRFSLIDDDGNPFYGERNIDIEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="104" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LMY9</accession>
    <name>Q1LMY9_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Putative AraC-type DNA-binding transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Rmet_1604</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08487.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516335.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1604"/>
    <dbReference type="EnsemblBacteria" id="ABF08487">
        <property type="protein sequence ID" value="ABF08487"/>
        <property type="gene ID" value="Rmet_1604"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1604"/>
    <dbReference type="eggNOG" id="COG4977">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_81_0_4"/>
    <dbReference type="OMA" id="AFMIRAC"/>
    <dbReference type="OrthoDB" id="1785082at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032783">
        <property type="entry name" value="AraC_lig"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018060">
        <property type="entry name" value="HTH_AraC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011051">
        <property type="entry name" value="RmlC_Cupin_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12852">
        <property type="entry name" value="Cupin_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12833">
        <property type="entry name" value="HTH_18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00342">
        <property type="entry name" value="HTH_ARAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51182">
        <property type="entry name" value="SSF51182"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01124">
        <property type="entry name" value="HTH_ARAC_FAMILY_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH araC/xylS-type" evidence="4">
        <location>
            <begin position="211"/>
            <end position="308"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS01124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABF08487.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="316" mass="33115" checksum="FB39A15BE6573CE4" modified="2006-05-30" version="1">MDSLSELVRLLAPAGTVDLHCRVAGAWSAHNEQAPPGHVPYHVILDGQASVQVGKETLQVGMGDVLLFPHGAAHTLHGMQGGRRRAAESRQFNGVVTEVSVPGAAAPLDILCGTFVLGSASKVLLSALPEMLCVGTRGNGDGEGEGGADDLAWLRGLIGMMHAEAERPGPGSAAIIADLSTALFTVLLRTLIAQGGVSQGVIALMADPRMARAVDAVVRAPEKPWTLDSLAQVCNVSRATLARRFSQLGMTPLELVTTLRMERAARLLRQDGLSAAAVAEQCGYASQAAFGRVFAQQFGIGPGAYRRQHRALRHGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LN37</accession>
    <name>Q1LN37_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Conjugal transfer transmembrane protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">trbL</name>
        <name evidence="7" type="ordered locus">Rmet_1556</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08439.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516293.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1556"/>
    <dbReference type="EnsemblBacteria" id="ABF08439">
        <property type="protein sequence ID" value="ABF08439"/>
        <property type="gene ID" value="Rmet_1556"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1556"/>
    <dbReference type="eggNOG" id="COG3846">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_049867_0_0_4"/>
    <dbReference type="OMA" id="WATRMKR"/>
    <dbReference type="OrthoDB" id="763833at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030255">
        <property type="term" value="P:protein secretion by the type IV secretion system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014150">
        <property type="entry name" value="Conjugal_tfr_TrbL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007688">
        <property type="entry name" value="Conjugal_tfr_TrbL/VirB6"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04610">
        <property type="entry name" value="TrbL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02783">
        <property type="entry name" value="TrbL_P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="29"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="68"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="146"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="176"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="207"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="240"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="273"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="390"/>
            <end position="461"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABF08439.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="461" mass="45811" checksum="009D021C06FEDE36" modified="2006-05-30" version="1">MNDVTIIDQFLNTFSTYIDSGFGLLRGEVAFLTATLIVIDMTIAGLYWAMSHATGQGEDVIAKLLRKVLYVGAFAYIINNFNWLAGIVFRSFAGLGITATGSAITMGNFLQPGRLAKAGIDAGAPILKQIGKMAGFPEVFVNLDPIVVMFLAWLVVVLCFFVLAIQLFITLIEFKLTTLAGFVLVPFALWNKTSFLAEKVLGNVVSAGVKVLVLAVIVGIGSGLFAQFQVHPAEPSIDHALVIMLASLTLLALGIFGPGIATGLVSGAPQLGAGAMAGAAVGAAGTAVAIGAAATGVGGAVAAGARMAPAAAKLAGSGARAATSAASSAKSAFQAGSAAAGGGAKGAMAGLGNVAKTGAQSAGRAAVSRASAAGQRMAAPFRAGWNGPAADSGATAGGAGASGQAATGEATSGAANAQPAWAKRLHRRQQLTHAATTTAHTLRGGDGGGSGQGPSLRDSDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LN67</accession>
    <name>Q1LN67_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">((NiFe) hydrogenase C-terminal protease)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">hoxW</name>
        <name evidence="2" type="ordered locus">Rmet_1526</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase A31 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08409.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516269.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1526"/>
    <dbReference type="MEROPS" id="A31.004"/>
    <dbReference type="EnsemblBacteria" id="ABF08409">
        <property type="protein sequence ID" value="ABF08409"/>
        <property type="gene ID" value="Rmet_1526"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1526"/>
    <dbReference type="eggNOG" id="COG0680">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_099037_6_0_4"/>
    <dbReference type="OMA" id="QDDGLGW"/>
    <dbReference type="OrthoDB" id="1858718at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008047">
        <property type="term" value="F:enzyme activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008233">
        <property type="term" value="F:peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1450">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023430">
        <property type="entry name" value="Pept_HybD-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000671">
        <property type="entry name" value="Peptidase_A31"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30302">
        <property type="entry name" value="PTHR30302"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53163">
        <property type="entry name" value="SSF53163"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00072">
        <property type="entry name" value="hydrog_prot"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF08409.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="168" mass="19208" checksum="14A32177881228AB" modified="2006-05-30" version="1">MNAPAEFPLVTLEDFDDPSSLIYGIGNVGRQDDGLGWALIDWLEAESLCPKAEIQRHYQLHLEDADLISRKRRVLFIDATKDASVESFTLERAEPKMDFSFTSHAISIPSIMATCQQCFQRLPEVYVLAIRGYEWELKMGLTQRARQNLDDATAYLAMRRSVESREFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="60" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LNB6</accession>
    <name>Q1LNB6_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">F plasmid protein 32-like protein (Protein belongs to CMGI-3)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">yupB</name>
        <name evidence="1" type="ordered locus">Rmet_1477</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08360.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516226.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1477"/>
    <dbReference type="EnsemblBacteria" id="ABF08360">
        <property type="protein sequence ID" value="ABF08360"/>
        <property type="gene ID" value="Rmet_1477"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1477"/>
    <dbReference type="eggNOG" id="COG1961">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_059317_0_0_4"/>
    <dbReference type="OMA" id="DSDIRIN"/>
    <dbReference type="OrthoDB" id="716673at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026325">
        <property type="entry name" value="DUF932"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06067">
        <property type="entry name" value="DUF932"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF08360.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="276" mass="30928" checksum="B64ED424BF6723C0" modified="2006-05-30" version="1">MQLASRFASRSPSLRSDYPLSDDQIRRVAPSIFADAPHESRSERYSYIPTAAVLTELRKEGFQPFMVTQTRVRDEGKREHTKHMLRLRHASQINGAEANEIVLLNSHDGTSSYQMLAGMFRFVCSNGLVCGDTVADVRVPHKGDVAGSVIEGAFEVLSGFERVKESRDAMRAITLDEGEAEVFARSALALKYDPTNNKPAPITESQILMPRRFDDSRPDLWSVFNRTQENLTKGGLHGRSANGRRQQTRPVQGIDSDVRLNRALWMLADGLRQLKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="61" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LNB8</accession>
    <name>Q1LNB8_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Lipoprotein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_1475</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08358.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516224.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1475"/>
    <dbReference type="EnsemblBacteria" id="ABF08358">
        <property type="protein sequence ID" value="ABF08358"/>
        <property type="gene ID" value="Rmet_1475"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1475"/>
    <dbReference type="eggNOG" id="COG3616">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_119528_1_1_4"/>
    <dbReference type="OMA" id="GEPEIGW"/>
    <dbReference type="OrthoDB" id="1558145at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021341">
        <property type="entry name" value="DUF2958"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11171">
        <property type="entry name" value="DUF2958"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF08358.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="116" mass="12620" checksum="7F1463137C60657C" modified="2006-05-30" version="1">MINALITDEQRIVLLANGRESLQNPDFDPAPVVKLFTPDAGATWLLTEIDPDDHDHAFGLCDLGLGEPEVGWVSLGELATVRGGLGLPIERDLSFRAEKRLSAYARDARLAGRVIV</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-01-15" modified="2022-05-25" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LNF7</accession>
    <name>EFTS_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Elongation factor Ts</fullName>
            <shortName evidence="1">EF-Ts</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">tsf</name>
        <name type="ordered locus">Rmet_1436</name>
    </gene>
    <organism>
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Associates with the EF-Tu.GDP complex and induces the exchange of GDP to GTP. It remains bound to the aminoacyl-tRNA.EF-Tu.GTP complex up to the GTP hydrolysis stage on the ribosome.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the EF-Ts family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08319.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516186.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q1LNF7"/>
    <dbReference type="SMR" id="Q1LNF7"/>
    <dbReference type="STRING" id="266264.Rmet_1436"/>
    <dbReference type="EnsemblBacteria" id="ABF08319">
        <property type="protein sequence ID" value="ABF08319"/>
        <property type="gene ID" value="Rmet_1436"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1436"/>
    <dbReference type="eggNOG" id="COG0264">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047155_0_2_4"/>
    <dbReference type="OMA" id="DAGMMDC"/>
    <dbReference type="OrthoDB" id="1405357at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.479.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00050">
        <property type="entry name" value="EF_Ts"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036402">
        <property type="entry name" value="EF-Ts_dimer_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001816">
        <property type="entry name" value="Transl_elong_EFTs/EF1B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014039">
        <property type="entry name" value="Transl_elong_EFTs/EF1B_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018101">
        <property type="entry name" value="Transl_elong_Ts_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009060">
        <property type="entry name" value="UBA-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11741">
        <property type="entry name" value="PTHR11741"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00889">
        <property type="entry name" value="EF_TS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46934">
        <property type="entry name" value="SSF46934"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54713">
        <property type="entry name" value="SSF54713"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00116">
        <property type="entry name" value="tsf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01127">
        <property type="entry name" value="EF_TS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0251">Elongation factor</keyword>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_1000006158" description="Elongation factor Ts">
        <location>
            <begin position="1"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="region of interest" description="Involved in Mg(2+) ion dislocation from EF-Tu" evidence="1">
        <location>
            <begin position="80"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00050"/>
        </source>
    </evidence>
    <sequence length="292" mass="30949" checksum="D65D5FAF33FEA8CD" modified="2006-05-30" version="1">MAAITASMVAELRAKTDAPMMECKKALTEADGDLGKAEELLRVKLGNKASKAASRVTAEGIVVSYIDGTTGALVELNCETDFVSKNDDFLGFGAKVAELVAKQNPADVAVLSALEMDGSTVDAVRSALIGKIGENMTIRRFVRYTNGGKLVSYLHGTRIGVMVEFDGDEAAAKDVAMHVAAMKPVSLSADQVPAELIAKERSIAEQKAAESGKPAEIVAKMVEGSVQKYLKEVSLLNQSFVKNDKQTVEQMLKGVNTTVKGFTLYVVGEGIEKKQDDFAAEVAAQVAAAQKA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-02-05" modified="2022-08-03" version="96" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LNJ3</accession>
    <name>ALR_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Alanine racemase</fullName>
            <ecNumber evidence="1">5.1.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">alr</name>
        <name type="ordered locus">Rmet_1400</name>
    </gene>
    <organism>
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the interconversion of L-alanine and D-alanine. May also act on other amino acids.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>L-alanine = D-alanine</text>
            <dbReference type="Rhea" id="RHEA:20249"/>
            <dbReference type="ChEBI" id="CHEBI:57416"/>
            <dbReference type="ChEBI" id="CHEBI:57972"/>
            <dbReference type="EC" id="5.1.1.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; D-alanine biosynthesis; D-alanine from L-alanine: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the alanine racemase family.</text>
    </comment>
    <dbReference type="EC" id="5.1.1.1" evidence="1"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08283.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516157.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q1LNJ3"/>
    <dbReference type="SMR" id="Q1LNJ3"/>
    <dbReference type="STRING" id="266264.Rmet_1400"/>
    <dbReference type="EnsemblBacteria" id="ABF08283">
        <property type="protein sequence ID" value="ABF08283"/>
        <property type="gene ID" value="Rmet_1400"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1400"/>
    <dbReference type="eggNOG" id="COG0787">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028393_1_0_4"/>
    <dbReference type="OMA" id="HMTHFSD"/>
    <dbReference type="OrthoDB" id="859043at2"/>
    <dbReference type="UniPathway" id="UPA00042">
        <property type="reaction ID" value="UER00497"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008784">
        <property type="term" value="F:alanine racemase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030632">
        <property type="term" value="P:D-alanine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.37.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01201">
        <property type="entry name" value="Ala_racemase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000821">
        <property type="entry name" value="Ala_racemase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009006">
        <property type="entry name" value="Ala_racemase/Decarboxylase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011079">
        <property type="entry name" value="Ala_racemase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001608">
        <property type="entry name" value="Ala_racemase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020622">
        <property type="entry name" value="Ala_racemase_pyridoxalP-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029066">
        <property type="entry name" value="PLP-binding_barrel"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00842">
        <property type="entry name" value="Ala_racemase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01168">
        <property type="entry name" value="Ala_racemase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00992">
        <property type="entry name" value="ALARACEMASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM01005">
        <property type="entry name" value="Ala_racemase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50621">
        <property type="entry name" value="SSF50621"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51419">
        <property type="entry name" value="SSF51419"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00492">
        <property type="entry name" value="alr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00395">
        <property type="entry name" value="ALANINE_RACEMASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0413">Isomerase</keyword>
    <keyword id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_1000066032" description="Alanine racemase">
        <location>
            <begin position="1"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor; specific for D-alanine" evidence="1">
        <location>
            <position position="35"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor; specific for L-alanine" evidence="1">
        <location>
            <position position="253"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="130"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="305"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="1">
        <location>
            <position position="35"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01201"/>
        </source>
    </evidence>
    <sequence length="368" mass="39528" checksum="59FA8BE9F087B6BB" modified="2006-05-30" version="1">MPRPIQAVIHQPALANNLEVVRRQAPASRIWAVVKANAYGHGIRRVFAGLKAADGFGLLDLSEAVLLRDLGWQGPILLLEGIFQAQDVPLLEQYRLTTAVHCEEQLRMLELARPKGPLAIQLKLNTGMNRLGFRPAQYRAAWERARTMSCIGSIVHMTHFSDADSKRGIAHQVEAFEAATANLPGEVSMANSAAVLWHPQAHRNWVRPGIILYGGSPTGVSSDIAETGLQPAMSLHSELIGIQDLQPGDTVGYGSMFTADRAMRVGVVACGYADGYPRHAVGWGERRAPVLVDGVRTQLVGRVSMDMICVDLTPCPKAKVGTPVTLWGHGLPIDDVAAASGTVGYELMCALAPRVPTSVATLTTADAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LNN3</accession>
    <name>Q1LNN3_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_1360</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08243.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516123.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1360"/>
    <dbReference type="EnsemblBacteria" id="ABF08243">
        <property type="protein sequence ID" value="ABF08243"/>
        <property type="gene ID" value="Rmet_1360"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1360"/>
    <dbReference type="HOGENOM" id="CLU_1766487_0_0_4"/>
    <dbReference type="OrthoDB" id="2232592at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="117"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="53"/>
            <end position="80"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF08243.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="147" mass="16405" checksum="39AF7CE696FD22AC" modified="2006-05-30" version="1">MVQLAMPCLFRGPVQTSSPRRPMEIDKFQPFVLDLYTQLGQVQRDIGSLTAGQQAMTEQLRQQESQMRESESRIIHAMDQFEARLTARVDKVESDLSGRIQSTEDKLSVMKDRVNRVFWATGGGGVVLGLALGWRPILARAASLLGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="61" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LNY7</accession>
    <name>Q1LNY7_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Replication initiator and transcription repressor protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">repA</name>
        <name evidence="1" type="ordered locus">Rmet_1254</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08139.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516026.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1254"/>
    <dbReference type="EnsemblBacteria" id="ABF08139">
        <property type="protein sequence ID" value="ABF08139"/>
        <property type="gene ID" value="Rmet_1254"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1254"/>
    <dbReference type="eggNOG" id="COG5534">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_050846_0_0_4"/>
    <dbReference type="OMA" id="DILIWAI"/>
    <dbReference type="OrthoDB" id="355274at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018777">
        <property type="entry name" value="Replication_initiator_prot_A"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10134">
        <property type="entry name" value="RPA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF08139.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="285" mass="32460" checksum="A7E7FE4083548953" modified="2006-05-30" version="1">MSSTALPMRQRPLQEREQLDLFRALPGDMAPRDSQDLMAYPFFSLAKSKRVKPIDFRAGNVTIRVEGTQEHGIATIWDADVLIWAASQIVEAKDAGLRPSRLMRATPYEILRFIGRGKSLRDYQRLKAALDRLQSTTVATSIRETTGRRLHRFSWINEWKELADASGTPLGIELILPDWFYAGVLDAALVLTIDPAYFRLTGGIERWLYRLVRKHGGKQAYGWQFDFRYLHQKSGSTAKPYDFACDLRALVARQSLPGYVLGIERMPDNGMELLTFRPVPQTAWG</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-10-07" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LPG9</accession>
    <name>Q1LPG9_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_1071</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07957.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1071"/>
    <dbReference type="EnsemblBacteria" id="ABF07957">
        <property type="protein sequence ID" value="ABF07957"/>
        <property type="gene ID" value="Rmet_1071"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1071"/>
    <dbReference type="HOGENOM" id="CLU_2384046_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF07957.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="94" mass="11289" checksum="3ECDF85ECC9C1123" modified="2006-05-30" version="1">MLWPLAVRKKKLLLPPLTRLLRLLLLRLKLRLRLLLRLLTLRLRLLLRLTLRLRLLTLRLRLPSSNRIRPIKKPTFGSAFFISRIFLTPHSRGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LPJ4</accession>
    <name>Q1LPJ4_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 9">Gluconokinase</fullName>
            <ecNumber evidence="4 9">2.7.1.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">gntK</name>
        <name evidence="10" type="ordered locus">Rmet_1046</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 9">
            <text>ATP + D-gluconate = 6-phospho-D-gluconate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19433"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:18391"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58759"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.12"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Carbohydrate acid metabolism.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the gluconokinase GntK/GntV family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.12" evidence="4 9"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07932.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515837.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1046"/>
    <dbReference type="EnsemblBacteria" id="ABF07932">
        <property type="protein sequence ID" value="ABF07932"/>
        <property type="gene ID" value="Rmet_1046"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1046"/>
    <dbReference type="eggNOG" id="COG3265">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_077168_4_0_4"/>
    <dbReference type="OMA" id="ENFIQVS"/>
    <dbReference type="OrthoDB" id="1843451at2"/>
    <dbReference type="UniPathway" id="UPA01057">
        <property type="reaction ID" value="UER00900"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046316">
        <property type="term" value="F:gluconokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02021">
        <property type="entry name" value="GntK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031322">
        <property type="entry name" value="Shikimate/glucono_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006001">
        <property type="entry name" value="Therm_gnt_kin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43442">
        <property type="entry name" value="PTHR43442"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01202">
        <property type="entry name" value="SKI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01313">
        <property type="entry name" value="therm_gnt_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 9 10" id="KW-0418">Kinase</keyword>
    <keyword evidence="6 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 9 10" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008420"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU363066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ABF07932.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="171" mass="18622" checksum="EBFB41C831A44F8D" modified="2006-05-30" version="1">MIYILMGVSGSGKSTVGQMLADRLHCGFHDADSFHSDANKAKMHAGIPLTDDDRWPWLDAMRAAIDAARAEGRTHVFTCSALRQVYRDRLLPADGGVTFVFMKGDASTIGSRLSARTDHFFNPALLQSQFETLEEPCDALVLDIRRTPEALVEDILEKCPPASAACSGKAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="98" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LPL0</accession>
    <name>Q1LPL0_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">2Fe-2S ferredoxin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">fdx</name>
        <name evidence="5" type="ordered locus">Rmet_1030</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>2Fe-2S cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49601"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the adrenodoxin/putidaredoxin family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07916.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515823.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1030"/>
    <dbReference type="EnsemblBacteria" id="ABF07916">
        <property type="protein sequence ID" value="ABF07916"/>
        <property type="gene ID" value="Rmet_1030"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1030"/>
    <dbReference type="eggNOG" id="COG0633">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_082632_5_2_4"/>
    <dbReference type="OMA" id="SACGGVC"/>
    <dbReference type="OrthoDB" id="1837979at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140647">
        <property type="term" value="P:P450-containing electron transport chain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00207">
        <property type="entry name" value="fer2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036010">
        <property type="entry name" value="2Fe-2S_ferredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001041">
        <property type="entry name" value="2Fe-2S_ferredoxin-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001055">
        <property type="entry name" value="Adrenodoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018298">
        <property type="entry name" value="Adrenodoxin_Fe-S_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012675">
        <property type="entry name" value="Beta-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011536">
        <property type="entry name" value="Fdx_isc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23426">
        <property type="entry name" value="PTHR23426"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00111">
        <property type="entry name" value="Fer2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00355">
        <property type="entry name" value="ADRENODOXIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54292">
        <property type="entry name" value="SSF54292"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02007">
        <property type="entry name" value="fdx_isc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51085">
        <property type="entry name" value="2FE2S_FER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00814">
        <property type="entry name" value="ADX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="2Fe-2S ferredoxin-type" evidence="4">
        <location>
            <begin position="8"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001939"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00019395"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABF07916.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="112" mass="12205" checksum="F05C69AD595608D0" modified="2006-05-30" version="1">MPQIIVLPHVELCPEGAVFEANTGTSVCDALLANGIEIEHACEKSCACTTCHVIVREGFNSLEDAEEKEEDLLDKAWGLEPNSRLSCQALVADDDLTVEIPKYTINHAKEGH</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="57" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LPN3</accession>
    <name>Q1LPN3_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_1007</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07893.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515807.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1007"/>
    <dbReference type="EnsemblBacteria" id="ABF07893">
        <property type="protein sequence ID" value="ABF07893"/>
        <property type="gene ID" value="Rmet_1007"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1007"/>
    <dbReference type="eggNOG" id="ENOG50315C6">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_120353_0_0_4"/>
    <dbReference type="OMA" id="TNPFLHM"/>
    <dbReference type="OrthoDB" id="1648106at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014993">
        <property type="entry name" value="DUF1841"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08897">
        <property type="entry name" value="DUF1841"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF07893.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="143" mass="16183" checksum="7AA1E5E7F41AD6A0" modified="2006-05-30" version="1">MFNPSREEVRRFFCEAWQKRVAGNVLTPLEAIAVDWIDQHPEYQSLLTDTEGALARDYTPEGGQTNPFLHLAMHLSISEQVSIDQPPGIRAAYEALSRRLDSPHEAQHQIMEALGQMLWNAQRNGMPPDGQAYVDDIRRRAAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LPX8</accession>
    <name>Q1LPX8_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Acetolactate synthase small subunit</fullName>
            <shortName evidence="8">AHAS</shortName>
            <shortName evidence="8">ALS</shortName>
            <ecNumber evidence="8">2.2.1.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Acetohydroxy-acid synthase small subunit</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="primary">ilvH</name>
        <name evidence="10" type="ordered locus">Rmet_0912</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Catalyzes the conversion of 2 pyruvate molecules into acetolactate in the first common step of the biosynthetic pathway of the branched-amino acids such as leucine, isoleucine, and valine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 8">
            <text>H(+) + 2 pyruvate = (2S)-2-acetolactate + CO2</text>
            <dbReference type="Rhea" id="RHEA:25249"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:58476"/>
            <dbReference type="EC" id="2.2.1.6"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 8">Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 1/4.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3 8">Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 1/4.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5 8">Dimer of large and small chains.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 8">Belongs to the acetolactate synthase small subunit family.</text>
    </comment>
    <dbReference type="EC" id="2.2.1.6" evidence="8"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07798.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008643328.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0912"/>
    <dbReference type="EnsemblBacteria" id="ABF07798">
        <property type="protein sequence ID" value="ABF07798"/>
        <property type="gene ID" value="Rmet_0912"/>
    </dbReference>
    <dbReference type="GeneID" id="60825400"/>
    <dbReference type="KEGG" id="rme:Rmet_0912"/>
    <dbReference type="eggNOG" id="COG0440">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_055003_1_3_4"/>
    <dbReference type="OMA" id="EPYGIKE"/>
    <dbReference type="OrthoDB" id="1500398at2"/>
    <dbReference type="UniPathway" id="UPA00047">
        <property type="reaction ID" value="UER00055"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00049">
        <property type="reaction ID" value="UER00059"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003984">
        <property type="term" value="F:acetolactate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990610">
        <property type="term" value="F:acetolactate synthase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009097">
        <property type="term" value="P:isoleucine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009099">
        <property type="term" value="P:valine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd04878">
        <property type="entry name" value="ACT_AHAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004789">
        <property type="entry name" value="Acetalactate_synth_ssu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027271">
        <property type="entry name" value="Acetolactate_synth/TF_NikR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019455">
        <property type="entry name" value="Acetolactate_synth_ssu_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045865">
        <property type="entry name" value="ACT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002912">
        <property type="entry name" value="ACT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039557">
        <property type="entry name" value="AHAS_ACT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30239">
        <property type="entry name" value="PTHR30239"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10369">
        <property type="entry name" value="ALS_ss_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55021">
        <property type="entry name" value="SSF55021"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00119">
        <property type="entry name" value="acolac_sm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51671">
        <property type="entry name" value="ACT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="7 8" id="KW-0100">Branched-chain amino acid biosynthesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 10" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="ACT" evidence="9">
        <location>
            <begin position="4"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006341"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011744"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU368092"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ABF07798.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="163" mass="17958" checksum="4486FC06C9F78E49" modified="2006-05-30" version="1">MRHIISVLLENEPGALSRVVGLFSARGYNIETLTVAPTEDASLSRMTIVTTGSDDVIEQITKHLNRLVEVVKVVDLTEGAHIERELMLVKVRAVGKEREEMKRTADIFRGRIIDVTEKTYTIELTGNGGKLDAFLDAIDRTAILETVRTGGSGIGRGERILKV</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-01-15" modified="2022-08-03" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LQ98</accession>
    <name>MGSA_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Methylglyoxal synthase</fullName>
            <shortName evidence="1">MGS</shortName>
            <ecNumber evidence="1">4.2.3.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">mgsA</name>
        <name type="ordered locus">Rmet_0792</name>
    </gene>
    <organism>
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the formation of methylglyoxal from dihydroxyacetone phosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>dihydroxyacetone phosphate = methylglyoxal + phosphate</text>
            <dbReference type="Rhea" id="RHEA:17937"/>
            <dbReference type="ChEBI" id="CHEBI:17158"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57642"/>
            <dbReference type="EC" id="4.2.3.3"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the methylglyoxal synthase family.</text>
    </comment>
    <dbReference type="EC" id="4.2.3.3" evidence="1"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07678.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515630.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q1LQ98"/>
    <dbReference type="SMR" id="Q1LQ98"/>
    <dbReference type="STRING" id="266264.Rmet_0792"/>
    <dbReference type="EnsemblBacteria" id="ABF07678">
        <property type="protein sequence ID" value="ABF07678"/>
        <property type="gene ID" value="Rmet_0792"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0792"/>
    <dbReference type="eggNOG" id="COG1803">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_120420_1_0_4"/>
    <dbReference type="OMA" id="RACDVHN"/>
    <dbReference type="OrthoDB" id="1726279at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008929">
        <property type="term" value="F:methylglyoxal synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019242">
        <property type="term" value="P:methylglyoxal biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01422">
        <property type="entry name" value="MGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1380">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00549">
        <property type="entry name" value="Methylglyoxal_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004363">
        <property type="entry name" value="Methylgl_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018148">
        <property type="entry name" value="Methylglyoxal_synth_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011607">
        <property type="entry name" value="MGS-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036914">
        <property type="entry name" value="MGS-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30492">
        <property type="entry name" value="PTHR30492"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02142">
        <property type="entry name" value="MGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006614">
        <property type="entry name" value="Methylglyox_syn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00851">
        <property type="entry name" value="MGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52335">
        <property type="entry name" value="SSF52335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00160">
        <property type="entry name" value="MGSA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01335">
        <property type="entry name" value="METHYLGLYOXAL_SYNTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51855">
        <property type="entry name" value="MGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0456">Lyase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_1000017824" description="Methylglyoxal synthase">
        <location>
            <begin position="1"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="domain" description="MGS-like" evidence="1">
        <location>
            <begin position="1"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="1">
        <location>
            <position position="64"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="12"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="16"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="38"/>
            <end position="41"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="58"/>
            <end position="59"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="91"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00549"/>
        </source>
    </evidence>
    <sequence length="140" mass="14934" checksum="0BEC4FB86A44B433" modified="2006-05-30" version="1">MRSKPRIALIAHDRKKDDIVAFAGRHRDFLAQCDLLATGTTGGRIAAETGLPVARMLSGPWGGDLQIGAQLAEGRVTAVVFLRDPMTPQPHEPDINALVRACDVHNVPCATNLATAELVVVELARICFEEDQAAADDAAP</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2007-02-06" modified="2022-08-03" version="98" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LQB5</accession>
    <name>PHNC1_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Phosphonates import ATP-binding protein PhnC 1</fullName>
            <ecNumber evidence="1">7.3.2.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">phnC1</name>
        <name type="ordered locus">Rmet_0775</name>
    </gene>
    <organism>
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Part of the ABC transporter complex PhnCDE involved in phosphonates import. Responsible for energy coupling to the transport system.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + H2O + phosphonate(out) = ADP + H(+) + phosphate + phosphonate(in)</text>
            <dbReference type="Rhea" id="RHEA:18065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16215"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="7.3.2.2"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">The complex is composed of two ATP-binding proteins (PhnC), two transmembrane proteins (PhnE) and a solute-binding protein (PhnD).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell inner membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the ABC transporter superfamily. Phosphonates importer (TC 3.A.1.9.1) family.</text>
    </comment>
    <comment type="sequence caution" evidence="2">
        <conflict type="erroneous initiation">
            <sequence resource="EMBL-CDS" id="ABF07661" version="1"/>
        </conflict>
    </comment>
    <dbReference type="EC" id="7.3.2.2" evidence="1"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07661.1"/>
        <property type="status" value="ALT_INIT"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_024570712.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q1LQB5"/>
    <dbReference type="SMR" id="Q1LQB5"/>
    <dbReference type="STRING" id="266264.Rmet_0775"/>
    <dbReference type="EnsemblBacteria" id="ABF07661">
        <property type="protein sequence ID" value="ABF07661"/>
        <property type="gene ID" value="Rmet_0775"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0775"/>
    <dbReference type="eggNOG" id="COG3638">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_22_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015416">
        <property type="term" value="F:ABC-type phosphonate transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03256">
        <property type="entry name" value="ABC_PhnC_transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012693">
        <property type="entry name" value="ABC_transpr_PhnC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02315">
        <property type="entry name" value="ABC_phnC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51249">
        <property type="entry name" value="PHNC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0997">Cell inner membrane</keyword>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0918">Phosphonate transport</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-1278">Translocase</keyword>
    <keyword id="KW-0813">Transport</keyword>
    <feature type="chain" id="PRO_0000274735" description="Phosphonates import ATP-binding protein PhnC 1">
        <location>
            <begin position="1"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="1">
        <location>
            <begin position="5"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="38"/>
            <end position="45"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01713"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="284" mass="30641" checksum="114F46FC4B5E0668" modified="2007-02-06" version="2">MTHAIEVRGLSKSFRADRKALDDVTLQIAPGEMVALLGASGSGKSTLLRHVAGFVTGDAGSGEILVNGRTVQRNGRLARDVRRVRADIGFVFQQFNLVGRLPVITNVLVGMLTRVPKWRSLLRIFKASEVQAGLDALAQVGIDDYAFQRASTLSGGQQQRAAIARTLVQNAQVILADEPIASLDPESSRRVMSQLAQINRTRKVAVVVSLHQVDVAMRYCPRVVALRHGKVVYDGPSAALTQQMLRDLYGSEADELLHDSVPEAPSCAEGVPAPVMVRMDLAAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LQM0</accession>
    <name>Q1LQM0_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Type IV pilus response regulator PilH</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">pilH</name>
        <name evidence="3" type="ordered locus">Rmet_0670</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07556.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008644878.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0670"/>
    <dbReference type="EnsemblBacteria" id="ABF07556">
        <property type="protein sequence ID" value="ABF07556"/>
        <property type="gene ID" value="Rmet_0670"/>
    </dbReference>
    <dbReference type="GeneID" id="60825148"/>
    <dbReference type="KEGG" id="rme:Rmet_0670"/>
    <dbReference type="eggNOG" id="COG0745">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_69_17_4"/>
    <dbReference type="OMA" id="VMCTSVE"/>
    <dbReference type="OrthoDB" id="1729688at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Response regulatory" evidence="2">
        <location>
            <begin position="5"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="1">
        <location>
            <position position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF07556.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="122" mass="13370" checksum="84EF3B5506263BEE" modified="2006-05-30" version="1">MTISKILIVDDSPTEALFMSDLLGKKGFKVSVAGNSDQAMARLEADAFDLILMDVVMPGQNGYQATRAIKRDDRFKDIPVIMCTSKGLETDRIWGMRQGASDYVVKPVDSEELLSKIAALAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="64" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LQQ6</accession>
    <name>Q1LQQ6_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">ImpA, N-terminal</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_0634</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07520.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515491.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0634"/>
    <dbReference type="EnsemblBacteria" id="ABF07520">
        <property type="protein sequence ID" value="ABF07520"/>
        <property type="gene ID" value="Rmet_0634"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0634"/>
    <dbReference type="eggNOG" id="COG3515">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026423_0_0_4"/>
    <dbReference type="OMA" id="AFAQADW"/>
    <dbReference type="OrthoDB" id="1255529at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010657">
        <property type="entry name" value="ImpA_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017739">
        <property type="entry name" value="T6SS-assoc_VCA0119"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06812">
        <property type="entry name" value="ImpA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16989">
        <property type="entry name" value="T6SS_VasJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03362">
        <property type="entry name" value="VI_chp_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ImpA_N" evidence="1">
        <location>
            <begin position="30"/>
            <end position="135"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF06812"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF07520.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="523" mass="57827" checksum="D42909ACCFB7F3BC" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LQW4</accession>
    <name>Q1LQW4_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Type-1 fimbrial protein subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">fimA</name>
        <name evidence="2" type="ordered locus">Rmet_0576</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07462.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515437.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0576"/>
    <dbReference type="EnsemblBacteria" id="ABF07462">
        <property type="protein sequence ID" value="ABF07462"/>
        <property type="gene ID" value="Rmet_0576"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0576"/>
    <dbReference type="eggNOG" id="COG3539">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_088965_2_4_4"/>
    <dbReference type="OMA" id="NIVDNTC"/>
    <dbReference type="OrthoDB" id="951513at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009289">
        <property type="term" value="C:pilus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007155">
        <property type="term" value="P:cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1090">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036937">
        <property type="entry name" value="Adhesion_dom_fimbrial_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008966">
        <property type="entry name" value="Adhesion_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039458">
        <property type="entry name" value="FimA-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16970">
        <property type="entry name" value="FimA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49401">
        <property type="entry name" value="SSF49401"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193446" evidence="1">
        <location>
            <begin position="25"/>
            <end position="182"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF07462.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="182" mass="18462" checksum="324A577577F6A2CA" modified="2006-05-30" version="1">MKHIRTKILATSLLLAALSPTAFAADGTIVFTGEIKGQTCSVAGEGRPAGDFTVVLPNVPSSLLNTGGNIAGNTPFAITLSNCTASLKKAAVYFEAGGNVDAVTGNLNNNAATNGAKNVQIALSNNDGSPIKLGAAYHAQNSKTVDIAGGNATLRYQAQYVATGTASAGLISTTLRYSVVYP</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="113" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LR21</accession>
    <name>Q1LR21_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 11">Tyrosine recombinase XerD</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11 15" type="primary">xerD</name>
        <name evidence="15" type="ordered locus">Rmet_0519</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Site-specific tyrosine recombinase, which acts by catalyzing the cutting and rejoining of the recombining DNA molecules. The XerC-XerD complex is essential to convert dimers of the bacterial chromosome into monomers to permit their segregation at cell division. It also contributes to the segregational stability of plasmids.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Forms a cyclic heterotetrameric complex composed of two molecules of XerC and two molecules of XerD.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the 'phage' integrase family. XerD subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07405.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515383.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0519"/>
    <dbReference type="EnsemblBacteria" id="ABF07405">
        <property type="protein sequence ID" value="ABF07405"/>
        <property type="gene ID" value="Rmet_0519"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0519"/>
    <dbReference type="eggNOG" id="COG4974">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027562_9_0_4"/>
    <dbReference type="OMA" id="QAFWYLI"/>
    <dbReference type="OrthoDB" id="745068at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009037">
        <property type="term" value="F:tyrosine-based site-specific recombinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007049">
        <property type="term" value="P:cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007059">
        <property type="term" value="P:chromosome segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006313">
        <property type="term" value="P:transposition, DNA-mediated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.443.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01808">
        <property type="entry name" value="Recomb_XerC_XerD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01807">
        <property type="entry name" value="Recomb_XerD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044068">
        <property type="entry name" value="CB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011010">
        <property type="entry name" value="DNA_brk_join_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013762">
        <property type="entry name" value="Integrase-like_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002104">
        <property type="entry name" value="Integrase_catalytic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010998">
        <property type="entry name" value="Integrase_recombinase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004107">
        <property type="entry name" value="Integrase_SAM-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011932">
        <property type="entry name" value="Recomb_XerD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023009">
        <property type="entry name" value="Tyrosine_recombinase_XerC/XerD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02899">
        <property type="entry name" value="Phage_int_SAM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00589">
        <property type="entry name" value="Phage_integrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56349">
        <property type="entry name" value="SSF56349"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02225">
        <property type="entry name" value="recomb_XerD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51900">
        <property type="entry name" value="CB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51898">
        <property type="entry name" value="TYR_RECOMBINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 11" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="5 11" id="KW-0132">Cell division</keyword>
    <keyword evidence="6 11" id="KW-0159">Chromosome partition</keyword>
    <keyword evidence="4 11" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 11" id="KW-0229">DNA integration</keyword>
    <keyword evidence="9 11" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="8 11 12" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Core-binding (CB)" evidence="14">
        <location>
            <begin position="6"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="domain" description="Tyr recombinase" evidence="13">
        <location>
            <begin position="112"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="active site" evidence="11">
        <location>
            <position position="152"/>
        </location>
    </feature>
    <feature type="active site" evidence="11">
        <location>
            <position position="177"/>
        </location>
    </feature>
    <feature type="active site" evidence="11">
        <location>
            <position position="248"/>
        </location>
    </feature>
    <feature type="active site" evidence="11">
        <location>
            <position position="251"/>
        </location>
    </feature>
    <feature type="active site" evidence="11">
        <location>
            <position position="274"/>
        </location>
    </feature>
    <feature type="active site" description="O-(3'-phospho-DNA)-tyrosine intermediate" evidence="11">
        <location>
            <position position="283"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010450"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00015810"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="ABF07405.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="302" mass="33986" checksum="D8864815AD5A5648" modified="2006-05-30" version="1">MTAALDGDLSLVDRFCDALWLEDGLARNTIDAYRRDLAMLARWLHESGTTALLGVDDTALSGYFAARHLETRASSANRRLTVFRRFYQWALREHMIEADPCLLLRPAKQPPRFPKTLSEAQVVALLEAPDIETPLGLRDRTMLELMYASGLRVSELTNMKTIEVGLNEGVARVVGGKGNKERLVPFGAQAGDWLHRYLSDARPTLLAGRACDELFVTQRGEGMTRQTFWHLIKKHARDAGIHAPLSPHTLRHAFATHLLNHGADLRVVQLLLGHADISTTQIYTHVARERLRELHLHHHPRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="65" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LR64</accession>
    <name>Q1LR64_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_0476</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07362.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0476"/>
    <dbReference type="EnsemblBacteria" id="ABF07362">
        <property type="protein sequence ID" value="ABF07362"/>
        <property type="gene ID" value="Rmet_0476"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0476"/>
    <dbReference type="eggNOG" id="ENOG503303I">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_135590_1_0_4"/>
    <dbReference type="OMA" id="QPEVYVE"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193776" evidence="2">
        <location>
            <begin position="27"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="135"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="137"/>
            <end position="154"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF07362.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="154" mass="16113" checksum="00B63A8B71CABD3A" modified="2006-05-30" version="1">MNGRKLCQIALVAAAALAFVSTSAQAHGGGHGGGGGHAHVGVGVVVGPGWGWGWGPGWGWGPGWYAPYGPYYYPGYYPPYYPPAAAVAPSEPPQYVEQGNVSNHGAPGDANAWWYHCSRPEGYYPYVKDCPGGWQRVPPQQPSSSNPSNPGVSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LR96</accession>
    <name>Q1LR96_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">1-Acylglycerol-3-phosphate O-acyltransferase</fullName>
            <ecNumber evidence="2">2.3.1.51</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">plsC</name>
        <name evidence="2" type="ordered locus">Rmet_0444</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.3.1.51" evidence="2"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07330.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515318.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0444"/>
    <dbReference type="EnsemblBacteria" id="ABF07330">
        <property type="protein sequence ID" value="ABF07330"/>
        <property type="gene ID" value="Rmet_0444"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0444"/>
    <dbReference type="eggNOG" id="COG0204">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027938_5_0_4"/>
    <dbReference type="OMA" id="MPRPLCY"/>
    <dbReference type="OrthoDB" id="1756450at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003841">
        <property type="term" value="F:1-acylglycerol-3-phosphate O-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002123">
        <property type="entry name" value="Plipid/glycerol_acylTrfase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01553">
        <property type="entry name" value="Acyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00563">
        <property type="entry name" value="PlsC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PlsC" evidence="1">
        <location>
            <begin position="75"/>
            <end position="191"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF07330.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="253" mass="28633" checksum="F1AD5A379BA6A4FD" modified="2006-05-30" version="1">MTFLRSLLFSLYLLALTPPYACLCFIVFPFMNAENRFRFVRGWTRLVIWAGRLICGIHYRIEGMEQAQSMLDKHVVLLSKHQSAWETVAYVALMPKPLCFVFKRELLLVPFFGWALGMLKMVHINRKDGPRAFASAARQGRDRLAEGAWIIMFPEGTRTASGLEKPRYKSGGARLAVETGAWVIPMAVNSGRLWPRNSFLKYPGMITISIGAPIASANKTADMLNQEVEAWIEAEMRRIDPDSYRRAGNQATA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LRE8</accession>
    <name>Q1LRE8_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">ABC-type nitrate/sulfonate/bicarbonate transport system, periplasmic component: TauT family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_0392</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07278.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515269.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0392"/>
    <dbReference type="EnsemblBacteria" id="ABF07278">
        <property type="protein sequence ID" value="ABF07278"/>
        <property type="gene ID" value="Rmet_0392"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0392"/>
    <dbReference type="eggNOG" id="COG0715">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028871_1_1_4"/>
    <dbReference type="OMA" id="DWKFEGP"/>
    <dbReference type="OrthoDB" id="1635706at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009228">
        <property type="term" value="P:thiamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027939">
        <property type="entry name" value="NMT1/THI5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015168">
        <property type="entry name" value="SsuA/THI5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006311">
        <property type="entry name" value="TAT_signal"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31528">
        <property type="entry name" value="PTHR31528"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09084">
        <property type="entry name" value="NMT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51318">
        <property type="entry name" value="TAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193380" evidence="1">
        <location>
            <begin position="34"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="domain" description="NMT1" evidence="2">
        <location>
            <begin position="53"/>
            <end position="267"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF09084"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF07278.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="352" mass="38107" checksum="39BEBBACBED53EDA" modified="2006-05-30" version="1">MPQSPLRRHLLKLCTAMGTTMVLSTLCAGTAQAQTATKIRFQLDWRFEGPSALFLLGEQKGYYKAEKLDVSIDAGNGSGNVVNRVASGTYDMGFADLASVMEFYGNNPDAKNKPVAVMMVYNNTPAAVLALKKSGIRAPKDLTGKKLGAPVFDAGRRAFPIFAKANGLQAASFNWQAMDPTLRETMLTRGDLDAITGFSFTSILNLNARGVKDEDIVVLPYPQYGVKLYGNAIIASEQFLKAHPEAVKAFLRAFAKSAKDVIANPEEGIKAVKARDGIIDEKLEVRRLKMALDAVVKSPDAKAEGFGRVNKPRLSLMASQVADAFATKGRINPDALWTDAYLPSAAELDVLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="51" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LRK0</accession>
    <name>Q1LRK0_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_0340</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07226.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515220.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0340"/>
    <dbReference type="EnsemblBacteria" id="ABF07226">
        <property type="protein sequence ID" value="ABF07226"/>
        <property type="gene ID" value="Rmet_0340"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0340"/>
    <dbReference type="eggNOG" id="ENOG5032YI0">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_189765_0_0_4"/>
    <dbReference type="OMA" id="ANHIGDH"/>
    <dbReference type="OrthoDB" id="2069137at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021233">
        <property type="entry name" value="DUF2783"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10932">
        <property type="entry name" value="DUF2783"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF07226.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="69" mass="7657" checksum="EFD003D19D0396D3" modified="2006-05-30" version="1">MPLNTAPNLAYPDEFYEALIEMHHDLDDAESQAVNARLILLLANHIGDLDTLREAMRLARDVALEPAAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LRK4</accession>
    <name>Q1LRK4_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Putative regulatory protein, IclR family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Rmet_0336</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07222.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515217.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0336"/>
    <dbReference type="EnsemblBacteria" id="ABF07222">
        <property type="protein sequence ID" value="ABF07222"/>
        <property type="gene ID" value="Rmet_0336"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0336"/>
    <dbReference type="eggNOG" id="COG1414">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_062618_2_0_4"/>
    <dbReference type="OMA" id="SRRFGWM"/>
    <dbReference type="OrthoDB" id="370329at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029016">
        <property type="entry name" value="GAF-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014757">
        <property type="entry name" value="Tscrpt_reg_IclR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005471">
        <property type="entry name" value="Tscrpt_reg_IclR_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09339">
        <property type="entry name" value="HTH_IclR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01614">
        <property type="entry name" value="IclR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00346">
        <property type="entry name" value="HTH_ICLR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51077">
        <property type="entry name" value="HTH_ICLR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51078">
        <property type="entry name" value="ICLR_ED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH iclR-type" evidence="3">
        <location>
            <begin position="16"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="domain" description="IclR-ED" evidence="4">
        <location>
            <begin position="78"/>
            <end position="262"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABF07222.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="268" mass="27603" checksum="BDBDB279D3B5741D" modified="2006-05-30" version="1">MSDDMLADAAKPQRGIQSLDNTGQLLGALVDAGQPLSLGELARAAGMVPAKAFPHLVSLQKIGLISRNADGDFQAGTLGLELGLIALQRLSPTREAEPEVIALAHATGLSVAMAVQGPLGPTVIRLEESARPQHVSLRVGTVLSMVNTAIGRVVAAHLPDDLLADALAQDAIRMAGTAFDGALPSGYLERLRGIRAGGIDHAVSRPVPGIDTLAAPVFDHTGALALVVAVMGSSGSFDSRLDSDTAHVVRQAARRLSWRFGASGATPA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-02-05" modified="2022-08-03" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LRP1</accession>
    <name>HPRK_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">HPr kinase/phosphorylase</fullName>
            <shortName evidence="1">HPrK/P</shortName>
            <ecNumber evidence="1">2.7.11.-</ecNumber>
            <ecNumber evidence="1">2.7.4.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">HPr(Ser) kinase/phosphorylase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">hprK</name>
        <name type="ordered locus">Rmet_0299</name>
    </gene>
    <organism>
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the ATP- as well as the pyrophosphate-dependent phosphorylation of a specific serine residue in HPr, a phosphocarrier protein of the phosphoenolpyruvate-dependent sugar phosphotransferase system (PTS). HprK/P also catalyzes the pyrophosphate-producing, inorganic phosphate-dependent dephosphorylation (phosphorolysis) of seryl-phosphorylated HPr (P-Ser-HPr).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>[HPr protein]-L-serine + ATP = [HPr protein]-O-phospho-L-serine + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46600"/>
            <dbReference type="Rhea" id="RHEA-COMP:11602"/>
            <dbReference type="Rhea" id="RHEA-COMP:11603"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>[HPr protein]-O-phospho-L-serine + H(+) + phosphate = [HPr protein]-L-serine + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:46604"/>
            <dbReference type="Rhea" id="RHEA-COMP:11602"/>
            <dbReference type="Rhea" id="RHEA-COMP:11603"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homohexamer.</text>
    </comment>
    <comment type="domain">
        <text evidence="1">The Walker A ATP-binding motif also binds Pi and PPi.</text>
    </comment>
    <comment type="miscellaneous">
        <text evidence="1">Both phosphorylation and phosphorolysis are carried out by the same active site and suggest a common mechanism for both reactions.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the HPrK/P family.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.-" evidence="1"/>
    <dbReference type="EC" id="2.7.4.-" evidence="1"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07185.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008650955.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q1LRP1"/>
    <dbReference type="SMR" id="Q1LRP1"/>
    <dbReference type="STRING" id="266264.Rmet_0299"/>
    <dbReference type="EnsemblBacteria" id="ABF07185">
        <property type="protein sequence ID" value="ABF07185"/>
        <property type="gene ID" value="Rmet_0299"/>
    </dbReference>
    <dbReference type="GeneID" id="60823025"/>
    <dbReference type="KEGG" id="rme:Rmet_0299"/>
    <dbReference type="eggNOG" id="COG1493">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_052030_0_2_4"/>
    <dbReference type="OMA" id="AMNNRQK"/>
    <dbReference type="OrthoDB" id="391150at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004712">
        <property type="term" value="F:protein serine/threonine/tyrosine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006109">
        <property type="term" value="P:regulation of carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01918">
        <property type="entry name" value="HprK_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1390.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01249">
        <property type="entry name" value="HPr_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003755">
        <property type="entry name" value="HPr(Ser)_kin/Pase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011104">
        <property type="entry name" value="Hpr_kin/Pase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011126">
        <property type="entry name" value="Hpr_kin/Pase_Hpr_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028979">
        <property type="entry name" value="Ser_kin/Pase_Hpr-like_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30305:SF1">
        <property type="entry name" value="PTHR30305:SF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07475">
        <property type="entry name" value="Hpr_kinase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02603">
        <property type="entry name" value="Hpr_kinase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75138">
        <property type="entry name" value="SSF75138"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00679">
        <property type="entry name" value="hpr-ser"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0418">Kinase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0511">Multifunctional enzyme</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_1000067169" description="HPr kinase/phosphorylase">
        <location>
            <begin position="1"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="region of interest" description="Important for the catalytic mechanism of both phosphorylation and dephosphorylation" evidence="1">
        <location>
            <begin position="209"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="region of interest" description="Important for the catalytic mechanism of dephosphorylation" evidence="1">
        <location>
            <begin position="271"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="146"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="167"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor; for phosphorylation activity. Proton donor; for dephosphorylation activity" evidence="1">
        <location>
            <position position="185"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="250"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="161"/>
            <end position="168"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="168"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="210"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01249"/>
        </source>
    </evidence>
    <sequence length="323" mass="35714" checksum="464B333F284C2C63" modified="2006-05-30" version="1">MELTGVTSQSIFDDNAADIKLSWVAGLEGADRAFDVEFAREATSAADLVGHLNLIHPNRIQVLGKPEILYYQRLDDEPRKRQMGELILLEPPFLVVADGMEPPPDLELRCTRSSTPLFTTPVSSAAVIDHLRLYLSRISAPRVTMHGVFLDILGMGVLIMGESGLGKSELGLELISRGHGLVADDAVDFVRLGPDFIEGRCPPLLQNLLEVRGLGLLDIKTIFGETAVRRKMKLKLVVQLVRRNDGEFERLPLDSQYLDVLGLPIHMVKIQVAAGRNLAVLVEAAVRNTILRLRGIDTLRDFMDRQRAAMQADVVSRGQGRLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="63" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LRR3</accession>
    <name>Q1LRR3_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">ABC-type transporter, periplasmic component: NitT family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">nasF</name>
        <name evidence="1" type="ordered locus">Rmet_0277</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07163.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515166.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0277"/>
    <dbReference type="EnsemblBacteria" id="ABF07163">
        <property type="protein sequence ID" value="ABF07163"/>
        <property type="gene ID" value="Rmet_0277"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0277"/>
    <dbReference type="eggNOG" id="COG0715">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037398_3_0_4"/>
    <dbReference type="OMA" id="GPKKDMA"/>
    <dbReference type="OrthoDB" id="1832232at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd13553">
        <property type="entry name" value="PBP2_NrtA_CpmA_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044527">
        <property type="entry name" value="NrtA/CpmA_ABC-bd_dom"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF07163.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="434" mass="47298" checksum="7082B37064EC21C5" modified="2006-05-30" version="1">MPSRLKVLKPQRANDTAAADAAPACHGRRRALATIAGASVMTLVDPLVRSGAWAAGSDAPEKPEVRIGFIPLTDCASVVMASTLGLDKKYGIKITPSKEASWAGVRDKLVNGELDAAHVLYGLIYGVQLGIGGPKKDMAVLMNLNHNGQAITLSSKLAEKGVKDGASLKALMSREKRDYVFAQTFPTGTHAMWLYYWLAANGIDPMRDAKAITVPPPQMVANMRVGNMDGFCVGEPWGARAIADKIGFTAETTQAIWKDHPEKTLGTTAEFVQKYPNTARAMVAAVLDASKFIDASASNRRKTAETIAAKSYVNTDMDIILDRMLGRYSNGLGKTWDDPDSMKFYQDGLANYPFLSDGMWFLTQHKRWGLLKEHPDYLAVAKQVNRIDIYKQAAVAAQVPLPKSDFRTARLIDGVTWDATKDPKAYADGFKIKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="92" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LS29</accession>
    <name>Q1LS29_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Isovaleryl CoA dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">aidB</name>
        <name evidence="8" type="ordered locus">Rmet_0161</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the acyl-CoA dehydrogenase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07047.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515073.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0161"/>
    <dbReference type="EnsemblBacteria" id="ABF07047">
        <property type="protein sequence ID" value="ABF07047"/>
        <property type="gene ID" value="Rmet_0161"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0161"/>
    <dbReference type="eggNOG" id="COG1960">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016513_0_0_4"/>
    <dbReference type="OMA" id="GNVQCLD"/>
    <dbReference type="OrthoDB" id="433393at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003995">
        <property type="term" value="F:acyl-CoA dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006089">
        <property type="entry name" value="Acyl-CoA_DH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006091">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036250">
        <property type="entry name" value="AcylCo_DH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009075">
        <property type="entry name" value="AcylCo_DH/oxidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009100">
        <property type="entry name" value="AcylCoA_DH/oxidase_NM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041504">
        <property type="entry name" value="AidB_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00441">
        <property type="entry name" value="Acyl-CoA_dh_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02770">
        <property type="entry name" value="Acyl-CoA_dh_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18158">
        <property type="entry name" value="AidB_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47203">
        <property type="entry name" value="SSF47203"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56645">
        <property type="entry name" value="SSF56645"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00073">
        <property type="entry name" value="ACYL_COA_DH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0274">FAD</keyword>
    <keyword evidence="3" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AidB_N" evidence="7">
        <location>
            <begin position="19"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA_dh_M" evidence="6">
        <location>
            <begin position="188"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA_dh_1" evidence="5">
        <location>
            <begin position="299"/>
            <end position="454"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF02770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF18158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ABF07047.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="561" mass="60867" checksum="43932F75C32EB127" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-07-28" modified="2022-08-03" version="92" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LS73</accession>
    <name>BIOB_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Biotin synthase</fullName>
            <ecNumber evidence="1">2.8.1.6</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">bioB</name>
        <name type="ordered locus">Rmet_0117</name>
    </gene>
    <organism>
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(4R,5S)-dethiobiotin + [sulfur carrier]-SH + 2 reduced [2Fe-2S]-[ferredoxin] + 2 S-adenosyl-L-methionine = 2 5'-deoxyadenosine + [sulfur carrier]-H + biotin + 2 L-methionine + 2 oxidized [2Fe-2S]-[ferredoxin]</text>
            <dbReference type="Rhea" id="RHEA:22060"/>
            <dbReference type="Rhea" id="RHEA-COMP:10000"/>
            <dbReference type="Rhea" id="RHEA-COMP:10001"/>
            <dbReference type="Rhea" id="RHEA-COMP:14737"/>
            <dbReference type="Rhea" id="RHEA-COMP:14739"/>
            <dbReference type="ChEBI" id="CHEBI:17319"/>
            <dbReference type="ChEBI" id="CHEBI:29917"/>
            <dbReference type="ChEBI" id="CHEBI:33737"/>
            <dbReference type="ChEBI" id="CHEBI:33738"/>
            <dbReference type="ChEBI" id="CHEBI:57586"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:64428"/>
            <dbReference type="ChEBI" id="CHEBI:149473"/>
            <dbReference type="EC" id="2.8.1.6"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="1">Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </cofactor>
        <text evidence="1">Binds 1 [2Fe-2S] cluster. The cluster is coordinated with 3 cysteines and 1 arginine.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; biotin biosynthesis; biotin from 7,8-diaminononanoate: step 2/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the radical SAM superfamily. Biotin synthase family.</text>
    </comment>
    <comment type="sequence caution" evidence="3">
        <conflict type="erroneous initiation">
            <sequence resource="EMBL-CDS" id="ABF07003" version="1"/>
        </conflict>
    </comment>
    <dbReference type="EC" id="2.8.1.6" evidence="1"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07003.1"/>
        <property type="status" value="ALT_INIT"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008642857.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q1LS73"/>
    <dbReference type="SMR" id="Q1LS73"/>
    <dbReference type="STRING" id="266264.Rmet_0117"/>
    <dbReference type="EnsemblBacteria" id="ABF07003">
        <property type="protein sequence ID" value="ABF07003"/>
        <property type="gene ID" value="Rmet_0117"/>
    </dbReference>
    <dbReference type="GeneID" id="60822839"/>
    <dbReference type="KEGG" id="rme:Rmet_0117"/>
    <dbReference type="eggNOG" id="COG0502">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033172_1_2_4"/>
    <dbReference type="OrthoDB" id="940969at2"/>
    <dbReference type="UniPathway" id="UPA00078">
        <property type="reaction ID" value="UER00162"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004076">
        <property type="term" value="F:biotin synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009102">
        <property type="term" value="P:biotin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01694">
        <property type="entry name" value="BioB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010722">
        <property type="entry name" value="BATS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002684">
        <property type="entry name" value="Biotin_synth/BioAB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024177">
        <property type="entry name" value="Biotin_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaB/NifB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22976">
        <property type="entry name" value="PTHR22976"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06968">
        <property type="entry name" value="BATS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001619">
        <property type="entry name" value="Biotin_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00272">
        <property type="entry name" value="biotin_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00876">
        <property type="entry name" value="BATS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00433">
        <property type="entry name" value="bioB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0001">2Fe-2S</keyword>
    <keyword id="KW-0004">4Fe-4S</keyword>
    <keyword id="KW-0093">Biotin biosynthesis</keyword>
    <keyword id="KW-0408">Iron</keyword>
    <keyword id="KW-0411">Iron-sulfur</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000381574" description="Biotin synthase">
        <location>
            <begin position="1"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="domain" description="Radical SAM core" evidence="2">
        <location>
            <begin position="66"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="81"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="85"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="88"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="125"/>
        </location>
        <ligand>
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="156"/>
        </location>
        <ligand>
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="216"/>
        </location>
        <ligand>
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="288"/>
        </location>
        <ligand>
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="345" mass="37484" checksum="BE1CEA1AD65D19E9" modified="2009-07-28" version="2">MTQQANQTVATISAEALRQSARNIAAAAPKEGDAWRVDDVAALFALPFNDLLFRAQQVHREHFDANTVQLSTLLSIKTGGCEEDCGYCPQSAHHDAGVKAEKLMDLEAVLDAAKAAKANGATRFCMGAAWREPKERHLEPVIDMVREVKAMGLETCVTLGMLKAEQAQRLKDAGLDYYNHNLDTSPEFYGKIITTRTYQDRLDTIGHVREAGINVCCGGIVGMGEAREARAGLIAQLANMDPYPESVPINNLVQVEGTPLAGTEALDPFEFVRTIAVARITMPRAMVRLSAGREAMDEALQALCFMAGANSIFYGEKLLTTGNPQADRDRALLARLDIRAEGYAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="63" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LSC0</accession>
    <name>Q1LSC0_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">ABC-type transporter, periplasmic component</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Rmet_0070</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF06956.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0070"/>
    <dbReference type="EnsemblBacteria" id="ABF06956">
        <property type="protein sequence ID" value="ABF06956"/>
        <property type="gene ID" value="Rmet_0070"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0070"/>
    <dbReference type="eggNOG" id="COG1463">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013850_1_1_4"/>
    <dbReference type="OMA" id="VPGQIIV"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003399">
        <property type="entry name" value="Mce/MlaD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02470">
        <property type="entry name" value="MlaD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="7"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="domain" description="MlaD" evidence="3">
        <location>
            <begin position="44"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="280"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="280"/>
            <end position="295"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF02470"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF06956.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="313" mass="33841" checksum="5D1379221F3D9A7B" modified="2006-05-30" version="1">MENKSNAFLAGVFTIGLAILVSVSLFWFSSDHTVRVPYDLITRSTVNGLGPQADVKYRGLEVGKVLSIKFDPQVPGQIIVRISINHDTPITRTTYATLGFQGVTGIAYVQLDDSERTEGDTSSSPRLTTSAREPARIVMRPGFFEELEKRGDSLLTQAETLMASLDSMFQPANRDELMAAIRAVHKTADDYSHLKSTLQPAAEKLPKAVDNLNAALVSTKRLTDEIGNPDGTVMRTVNRVGNDLQVAADSVQSTTTIVNQETLPQINGLARESRQAVRSIDRAASQFNDKPSSVLFGGPSPTPGPGEPGFQAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-01-04" modified="2021-06-02" version="63" xmlns="http://uniprot.org/uniprot">
    <accession>Q5NUV7</accession>
    <name>Q5NUV7_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_6229</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="1 2">
        <name>pMOL28</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid pMOL28</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000355">
        <property type="protein sequence ID" value="ABF13088.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011239953.1">
        <property type="nucleotide sequence ID" value="NC_007972.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_161692.1">
        <property type="nucleotide sequence ID" value="NC_006525.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF13088">
        <property type="protein sequence ID" value="ABF13088"/>
        <property type="gene ID" value="Rmet_6229"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6229"/>
    <dbReference type="HOGENOM" id="CLU_162029_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid pMOL28"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF13088.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="117" mass="12366" checksum="11832FAD3C0F49F7" modified="2005-01-04" version="1">MPITIDGAGLAGSPGPLRIAVELYAVNDRAALSLVETKTGDLYETFTSNVPSETLADNEVIVCLWRLPMSTVVALLDSDCFEPTRTLAVGNARGAVWRITSVDLLQAIHDAKAGVPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-01-04" modified="2021-09-29" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>Q5NUY1</accession>
    <name>Q5NUY1_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">DNA repair exonuclease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">sbcD</name>
        <name evidence="1" type="ordered locus">Rmet_6220</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="1 2">
        <name>pMOL28</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid pMOL28</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000355">
        <property type="protein sequence ID" value="ABF13079.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011239960.1">
        <property type="nucleotide sequence ID" value="NC_007972.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_161699.1">
        <property type="nucleotide sequence ID" value="NC_006525.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF13079">
        <property type="protein sequence ID" value="ABF13079"/>
        <property type="gene ID" value="Rmet_6220"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6220"/>
    <dbReference type="HOGENOM" id="CLU_578326_0_0_4"/>
    <dbReference type="OMA" id="NGCQTEH"/>
    <dbReference type="OrthoDB" id="1831911at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid pMOL28"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004527">
        <property type="term" value="F:exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="SSF56300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0540">Nuclease</keyword>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF13079.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="482" mass="51509" checksum="D5C488D366CDCB4F" modified="2005-01-04" version="1">MLEDGESLLAAHFSDLHYSPGHLAEADRCFGFAVEDAIANRCRVAVVSGDSTDHRLDAHTPALSALARRIHQLSVHMPVVMLQGTFSHEPPGTLDLFRLIGASFPVYVADRIHQVALVGRTFIPSDGAIFSHAEMDKLLTQQGLPDVVFTCVPTVNKAVLAAAAGVANAATAVGDHLADYLAAAGTVNRQWRSHGVRTIGVSHGTVNGCQTEHGVPMAGLDHEFTIGALFEAHCDAFMLGHIHRAQQWEREGRVVAYPGSIGRFHYGEIGAKGYLQWQVSPGEAVAIPVKTPASETVCIDFDGPPDMAKLAEMAADAAHKFVRVRWTVNEEHRQLVDREAIEALFVGSAEVKLEARVLPAVRSRAEGISRAATLSAKLGRWCELTGVDANPLLDSLSMLQNLEAQAIADQVLAGLDEMSMPVTQKVGAVEAVAPPFVAKLTDMELEEPSAAPEAFPVAAAEPVAPIATASAPMNWLTDDLFA</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-03-23" modified="2020-10-07" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>D3DXL1</accession>
    <name>D3DXL1_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_6413</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ADC45031.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADC45031">
        <property type="protein sequence ID" value="ADC45031"/>
        <property type="gene ID" value="Rmet_6413"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6413"/>
    <dbReference type="HOGENOM" id="CLU_2993474_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="16"/>
            <end position="34"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADC45031.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="57" mass="6325" checksum="C2E31D62CE05A186" modified="2010-03-23" version="1">MASIIGGPSEHNDAKFSLEWIGIVILFYWLRIFGNDLARRASGRAPGDNPRHGNQMA</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-03-23" modified="2020-10-07" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>D3DXL6</accession>
    <name>D3DXL6_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_6418</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ADC45036.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADC45036">
        <property type="protein sequence ID" value="ADC45036"/>
        <property type="gene ID" value="Rmet_6418"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6418"/>
    <dbReference type="HOGENOM" id="CLU_3011044_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="35"/>
            <end position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADC45036.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="56" mass="6401" checksum="8BE0A2BD8D1A33CD" modified="2010-03-23" version="1">MPLVKGDPRGIARARKLPKATIRNMKQNLGFAFVRRPVPTSQNKKPQSREGLRLFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-03-23" modified="2020-10-07" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>D3DXV2</accession>
    <name>D3DXV2_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_6514</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ADC45122.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_6514"/>
    <dbReference type="EnsemblBacteria" id="ADC45122">
        <property type="protein sequence ID" value="ADC45122"/>
        <property type="gene ID" value="Rmet_6514"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6514"/>
    <dbReference type="HOGENOM" id="CLU_2754980_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADC45122.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="70" mass="8204" checksum="7BDF33FD4909DD29" modified="2010-03-23" version="1">MSTGYFNPPEEPLPPYVVEEGTACRTCSRLKIEVLQETPHPRLYLYCDFEDRKSMPRRSCEFYAREPGTD</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-03-23" modified="2020-10-07" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>D3DY66</accession>
    <name>D3DY66_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_6636</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ADC45236.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_6636"/>
    <dbReference type="EnsemblBacteria" id="ADC45236">
        <property type="protein sequence ID" value="ADC45236"/>
        <property type="gene ID" value="Rmet_6636"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6636"/>
    <dbReference type="HOGENOM" id="CLU_2668427_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADC45236.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="75" mass="8530" checksum="C107935315B18C05" modified="2010-03-23" version="1">MFFIMETDYGSAKDDLVIRFIQNQGCDRPIWYRYAFAKRPLPASLLTPPASGSLSSCCYPFGRPPPRHPCCALRM</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="70" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LAW5</accession>
    <name>Q1LAW5_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Rmet_5852</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="6">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="6">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12711.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518059.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5852"/>
    <dbReference type="EnsemblBacteria" id="ABF12711">
        <property type="protein sequence ID" value="ABF12711"/>
        <property type="gene ID" value="Rmet_5852"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5852"/>
    <dbReference type="eggNOG" id="COG3394">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_064244_4_0_4"/>
    <dbReference type="OrthoDB" id="1204930at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011330">
        <property type="entry name" value="Glyco_hydro/deAcase_b/a-brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006879">
        <property type="entry name" value="YdjC-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31609">
        <property type="entry name" value="PTHR31609"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04794">
        <property type="entry name" value="YdjC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88713">
        <property type="entry name" value="SSF88713"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="3" id="KW-0460">Magnesium</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0614">Plasmid</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABF12711.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="253" mass="28411" checksum="97B5B34068FB952E" modified="2006-05-30" version="1">MKGVIINADDFGYDDDTFQATVACFDAGLLTSATILAGSPATAHACQYARDNHSRFSFGLHFNIVEGRRPHGASSTLCRKDGSLFPPKIQRARAMAGLIDAADIRREFRLQLDELRDHGVNVSHVDGHGHLHKYPAVMRVLRDEMLRAGIERVRPPQNCYFLKNHMRGAMNVMLLRHFNGLRSPDFYVGADTRQQGWALRLPAMLPEGVTEMSVHPGFAEPWRREEGEPLMHPTPLATAFRQAGVVLMRYHDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LB02</accession>
    <name>Q1LB02_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Putative xanthine/uracil permease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Rmet_5815</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="9">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="9">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the nucleobase:cation symporter-2 (NCS2) (TC 2.A.40) family. Azg-like subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12674.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5815"/>
    <dbReference type="EnsemblBacteria" id="ABF12674">
        <property type="protein sequence ID" value="ABF12674"/>
        <property type="gene ID" value="Rmet_5815"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5815"/>
    <dbReference type="eggNOG" id="COG2252">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024508_0_1_4"/>
    <dbReference type="OMA" id="RKGSYFF"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015205">
        <property type="term" value="F:nucleobase transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904823">
        <property type="term" value="P:purine nucleobase transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045018">
        <property type="entry name" value="Azg-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026033">
        <property type="entry name" value="Azg-like_bact_archaea"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006043">
        <property type="entry name" value="NCS2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43337">
        <property type="entry name" value="PTHR43337"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00860">
        <property type="entry name" value="Xan_ur_permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005353">
        <property type="entry name" value="PbuG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0614">Plasmid</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="41"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="71"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="121"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="151"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="189"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="214"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="256"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="337"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="402"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="434"/>
            <end position="450"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ABF12674.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="453" mass="47256" checksum="DF8635CC991DBF46" modified="2006-05-30" version="1">MSTSDQRPRPGSESTLAQPSTGVLERLFKLGEHKTDARTEILAGVTTFLTMAYIIFVNPSILGDAGVPKDAVFVATCLAAAIGTLIMGFYANYPIAMAPGMGLNAYFAYTVVKGMGLPWEAALGAVFISGCLFLLVTLFRVREMIVNGIPHALRVAITAGIGLFLAIVALKNAGIITASPATLVTIGDLHQTSAILAVIGFFAIVALDHLKVKGAILIGILGTTILSFVFGGNTFHGVFSAPPSIAPTLFKLDISAALSIGIVNVVLVFFLVELFDATGTLMGVANRAGLLKAGRMDRLNKALMADSTAIMAGSLLGTSSTTAYIESASGVQAGGRTGLTAVTVAVLFLACLFIAPLAGTVPAYATAPALLYVSCLMLRELVDVDWSDATEVVPAVLTALGMPFTYSVANGVAFGFIAYAGLKLLTGRAREVPVMAWVIAAVFLFKFYYLPGH</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LB50</accession>
    <name>Q1LB50_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative transcriptional regulator with cAMP binding domain belongs to HTH Crp family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_5767</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12626.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5767"/>
    <dbReference type="EnsemblBacteria" id="ABF12626">
        <property type="protein sequence ID" value="ABF12626"/>
        <property type="gene ID" value="Rmet_5767"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5767"/>
    <dbReference type="eggNOG" id="COG0664">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_075053_1_1_4"/>
    <dbReference type="OMA" id="NERMGQF"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00038">
        <property type="entry name" value="CAP_ED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018490">
        <property type="entry name" value="cNMP-bd-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012318">
        <property type="entry name" value="HTH_CRP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00027">
        <property type="entry name" value="cNMP_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13545">
        <property type="entry name" value="HTH_Crp_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00419">
        <property type="entry name" value="HTH_CRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51206">
        <property type="entry name" value="SSF51206"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="2">
        <location>
            <begin position="39"/>
            <end position="142"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF12626.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="253" mass="27920" checksum="CF25EB13D5410035" modified="2006-05-30" version="1">MTDLAFSMRCSIVNAQKIDSYQMSSSLDQVNALLAQHDWFCALSPAHQALAASETVVREYPAGGYIARRGEFSRYWIGVDTGLIKLAVYTPDGRGCTFSGVPAGGWCGEGSVIKREDRRYDVIAIRDSRVLLVPEAVFNMLLSDSLPFAGFVVRQLNERMGQFIATVQNDRLLGADARVAQAVAQLFHPALYPKTTEVLELSQEEIGLLTGLSRQRVNQALRRLEAGGMVSLAYQTIRVTDLEGLRRFGLSEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="62" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LB58</accession>
    <name>Q1LB58_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_5759</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12618.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011520157.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5759"/>
    <dbReference type="EnsemblBacteria" id="ABF12618">
        <property type="protein sequence ID" value="ABF12618"/>
        <property type="gene ID" value="Rmet_5759"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5759"/>
    <dbReference type="eggNOG" id="COG2866">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_055736_0_0_4"/>
    <dbReference type="OMA" id="GFAWHRR"/>
    <dbReference type="OrthoDB" id="1584387at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021259">
        <property type="entry name" value="DUF2817"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10994">
        <property type="entry name" value="DUF2817"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF12618.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="369" mass="41163" checksum="DDF7F114CA5D8754" modified="2006-05-30" version="1">MESALHHRGVKVQPEVRNLFSRTYAEARQKFLQAAASRGLDVESHELPLDGAHGETLATDVVRDGPLHASKLVIIISGVHGVEGYCGSAIQTGLLGLGPVAAPDTAVLYIHAVNPHGFSYSRRETQENVDLNRNFVDFSAPLPDNKPYAEIHELLLPREWPPGDDNELALFMLRKEWGVKGFQRAVSLGQYRFEDGMHFGGREPAWSNTTFRRILQRHARNCQHLGSIDVHTGLGPYGVGERIFACLDEGRSLERARRWWGDVTSVHAGTSNSIPMTGPIQFALFDECAQAEQTNLCLEFGTYPMPQVIGASRAEHWLHRHGSSDPLQAAAIRQTLKDAFYPQADDWQQAVWQQGRDVFLQTLNGLRED</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LB69</accession>
    <name>Q1LB69_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_5748</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12607.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011520148.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5748"/>
    <dbReference type="EnsemblBacteria" id="ABF12607">
        <property type="protein sequence ID" value="ABF12607"/>
        <property type="gene ID" value="Rmet_5748"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5748"/>
    <dbReference type="eggNOG" id="COG0697">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033863_5_2_4"/>
    <dbReference type="OMA" id="MYVVVKV"/>
    <dbReference type="OrthoDB" id="1425840at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000620">
        <property type="entry name" value="EamA_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00892">
        <property type="entry name" value="EamA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="36"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="66"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="95"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="125"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="149"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="180"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="216"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="248"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="272"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="domain" description="EamA" evidence="2">
        <location>
            <begin position="9"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="domain" description="EamA" evidence="2">
        <location>
            <begin position="149"/>
            <end position="289"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF12607.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="303" mass="31941" checksum="0C193CF1E9EAAB53" modified="2006-05-30" version="1">MRPADLFRLLLLSAIWGASFLFIRIGAPVLGPLPTAFFRVLIGAVTLGACLPMLGLKWDMQGKWRAVIILGIINSGIPFVMYAIAALWLPAGYSAIFNAMTPLMGVLIGSLFFAEKLTRAKAAGVLMGLAGVGVLTRTGPVVFSHEVLLGALACLVATSCYGLAGFLTRRWITARGGLDSRLVAAGSLVGATLFLLPFSAVALWRENTLPAAGPGVWAAMLAVGMFCTAIAYVLYFRLIADLGPVRSLTVTFLIPPFGIVWGWMFLGESLSWAHAAGMALIGVAVWLVLRPAAPAVAPAPARS</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="69" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LBE3</accession>
    <name>Q1LBE3_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Sulf_transp domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">Rmet_5674</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="10">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="10">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the UPF0394 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12533.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011520079.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5674"/>
    <dbReference type="EnsemblBacteria" id="ABF12533">
        <property type="protein sequence ID" value="ABF12533"/>
        <property type="gene ID" value="Rmet_5674"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5674"/>
    <dbReference type="eggNOG" id="COG2391">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037802_2_1_4"/>
    <dbReference type="OMA" id="WGIAGFC"/>
    <dbReference type="OrthoDB" id="1518604at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007272">
        <property type="entry name" value="Sulf_transp"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04143">
        <property type="entry name" value="Sulf_transp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0614">Plasmid</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="41"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="80"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="112"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="domain" description="Sulf_transp" evidence="8">
        <location>
            <begin position="46"/>
            <end position="130"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009590"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF04143"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ABF12533.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="143" mass="14655" checksum="33E47AF33F689B73" modified="2006-05-30" version="1">MSAITALLAGLLFGIGLMVSGMANPAKVLGFLDLAGRWDPSLAFVMVGAIAIGSAAFLLAKRRQRSLLGLPMQLPTNTVITPRLVIGSALFGIGWGTAGFCPGPALVALGAGYPKAIGFVLAMAAGMLVFEVLERSKARVARA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="70" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LBQ8</accession>
    <name>Q1LBQ8_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_5559</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12418.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519965.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5559"/>
    <dbReference type="EnsemblBacteria" id="ABF12418">
        <property type="protein sequence ID" value="ABF12418"/>
        <property type="gene ID" value="Rmet_5559"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5559"/>
    <dbReference type="eggNOG" id="COG0210">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_387748_0_0_4"/>
    <dbReference type="OMA" id="FECARTA"/>
    <dbReference type="OrthoDB" id="137860at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011528">
        <property type="entry name" value="NERD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018647">
        <property type="entry name" value="SLFN_3-like_DNA/RNA_helicase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09848">
        <property type="entry name" value="DUF2075"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08378">
        <property type="entry name" value="NERD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NERD" evidence="1">
        <location>
            <begin position="56"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="domain" description="DUF2075" evidence="2">
        <location>
            <begin position="251"/>
            <end position="450"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF08378"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF09848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF12418.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="800" mass="90168" checksum="40607046BFC6EADF" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LBS1</accession>
    <name>Q1LBS1_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Putative vanillate O-demethylase oxygenase subunit (4-hydroxy-3-methoxybenzoate demethylase)</fullName>
            <ecNumber evidence="7">1.14.13.82</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">vanA</name>
        <name evidence="7" type="ordered locus">Rmet_5546</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="8">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="8">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EC" id="1.14.13.82" evidence="7"/>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12405.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519949.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="SMR" id="Q1LBS1"/>
    <dbReference type="STRING" id="266264.Rmet_5546"/>
    <dbReference type="EnsemblBacteria" id="ABF12405">
        <property type="protein sequence ID" value="ABF12405"/>
        <property type="gene ID" value="Rmet_5546"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5546"/>
    <dbReference type="eggNOG" id="COG4638">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039484_0_0_4"/>
    <dbReference type="OMA" id="WVHQSSF"/>
    <dbReference type="OrthoDB" id="691776at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018489">
        <property type="term" value="F:vanillate monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019439">
        <property type="term" value="P:aromatic compound catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.102.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017941">
        <property type="entry name" value="Rieske_2Fe-2S"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036922">
        <property type="entry name" value="Rieske_2Fe-2S_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044043">
        <property type="entry name" value="VanA_C_cat"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00355">
        <property type="entry name" value="Rieske"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19112">
        <property type="entry name" value="VanA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50022">
        <property type="entry name" value="SSF50022"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51296">
        <property type="entry name" value="RIESKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0058">Aromatic hydrocarbons catabolism</keyword>
    <keyword evidence="4" id="KW-0408">Iron</keyword>
    <keyword evidence="5" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="3 7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-0614">Plasmid</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Rieske" evidence="6">
        <location>
            <begin position="7"/>
            <end position="109"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022797"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABF12405.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="347" mass="39310" checksum="4C10E07F23434FA5" modified="2006-05-30" version="1">MYLKNAWYVAGWNSEFQKDQLVSRRLLGQDIVFYRRGDGSVAALEDRCCHRAAPLSKGQQEGDQIRCMYHGLLFNCAGQCVEVPGQERISDKLKVRSFPTEVRDNLVWIWMGQPELADASEIKQVHWHNREGWRVEEGGYLRYASGVQLIVDNLLDFSHLGFVHNKSIGTRKQGNIRPETEFQGETVSVRFTTLGSPPPAFARELGQLPENVDRFNYYVWHIRGNLFVQDSVIAPVGEGYESLDPATMKLHTFIALTPCDENTAHYFWATAHNNFTSTTEDLTRRLTEQVAHAFDEDREIIEAQQAVISTAPDAPMVAIAADGTLLKVRRMLDALLLEERSPKAATQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-10-07" version="39" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LC01</accession>
    <name>Q1LC01_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_5466</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12325.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5466"/>
    <dbReference type="EnsemblBacteria" id="ABF12325">
        <property type="protein sequence ID" value="ABF12325"/>
        <property type="gene ID" value="Rmet_5466"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5466"/>
    <dbReference type="HOGENOM" id="CLU_2234307_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF12325.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="105" mass="12020" checksum="288C19D58C99F368" modified="2006-05-30" version="1">MLPVAKGLFGSRRQDRTLWIAGVDCAGVPTISPHVSQTATRKFFTEPLLFANARLRRRKVVATTLPQREQIALLLFAFPEMLKTFLDRTELEIPKRCREFSLYAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LC99</accession>
    <name>Q1LC99_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Rmet_5368</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="5">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="5">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PrpD family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12227.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519774.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5368"/>
    <dbReference type="EnsemblBacteria" id="ABF12227">
        <property type="protein sequence ID" value="ABF12227"/>
        <property type="gene ID" value="Rmet_5368"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5368"/>
    <dbReference type="eggNOG" id="COG2079">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026574_3_1_4"/>
    <dbReference type="OMA" id="AFRMDSM"/>
    <dbReference type="OrthoDB" id="688128at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.4100.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036148">
        <property type="entry name" value="MmgE/PrpD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042183">
        <property type="entry name" value="MmgE/PrpD_sf_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042188">
        <property type="entry name" value="MmgE/PrpD_sf_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005656">
        <property type="entry name" value="MmgE_PrpD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045337">
        <property type="entry name" value="MmgE_PrpD_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045336">
        <property type="entry name" value="MmgE_PrpD_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16943">
        <property type="entry name" value="PTHR16943"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03972">
        <property type="entry name" value="MmgE_PrpD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19305">
        <property type="entry name" value="MmgE_PrpD_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103378">
        <property type="entry name" value="SSF103378"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0614">Plasmid</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MmgE_PrpD" evidence="2">
        <location>
            <begin position="5"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="domain" description="MmgE_PrpD_C" evidence="3">
        <location>
            <begin position="266"/>
            <end position="436"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF03972"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF19305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF12227.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="451" mass="47481" checksum="D9ED6292A01BDFC7" modified="2006-05-30" version="1">MLIDDFAEYVGDQARATLADDVMHHARRAVIDWFAALMAGVPMAPGRQLVAAHQTETGVGRSTLVGHATTAFPGLAAWINGSASHAAEFDDIFRDAIYHPGCPTIAAALALAEHRDASGTDFLRAVIAGYEVSTRIGAAIQPAHYRYFHTTGTVGCIGAAAAGSVLTAPGNARAAADAMATATTFASGLQQAFRSDAMTKALHAGHAAQVGVQAAQGAAHGVTGVHDILEGDAGFGAAMCGSPDWHGVTNGLGTDFNITRITQKNHGCCGHTFASIDAAMRIMRDNALAPARIAAVNVRTYQVALDVTGNFDPKTPFEARFSLPYVVAHGILHGAVRLDAFEPARLADASIRTLMSKIRLTADPELTAAFPKQRAARVEIEVTDGSRHAHFAPYRLGDPEAPLDDATLGDKFMELSAPALGSVHAARLLDALWRLDDRPLRSLALDKLQMG</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-06-17" version="57" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LCC2</accession>
    <name>Q1LCC2_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Transcription regulator XpsR</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">xpsR</name>
        <name evidence="1" type="ordered locus">Rmet_5345</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12204.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF12204">
        <property type="protein sequence ID" value="ABF12204"/>
        <property type="gene ID" value="Rmet_5345"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5345"/>
    <dbReference type="HOGENOM" id="CLU_076550_0_0_4"/>
    <dbReference type="OMA" id="WQSHQKI"/>
    <dbReference type="OrthoDB" id="1964917at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF12204.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="275" mass="29854" checksum="57A73D3CEA5D5470" modified="2006-05-30" version="1">MEGCSSWGGYPAFISTIPAAQSDRAGVSAGDRKPGLDAFLASYAGMQGNPESPVWLCEATSRLAGSPIAASPEPLQMPVVWNADFRQRNQDEMPKWQSHQKIARILAAVHTPATDPGAPDWKEYFQERLYGPAGNEFWLSLYPALARTEDSASGRSPHDTFAVPRTLPRYAALCRQGGRFEFVTRLARHMAPKIIVCFGHQHSDDYQSAFGFAGVVGEEVILQPADLPKRLRVFTKDGTKLVIVPLLGGTDGINSDVLQIALGRYIGRLLSAGAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LCG6</accession>
    <name>Q1LCG6_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 12">Flagellar biosynthetic protein FliP</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12 13" type="primary">fliP</name>
        <name evidence="13" type="ordered locus">Rmet_5301</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="14">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="14">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Plays a role in the flagellum-specific transport system.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="12">Cell membrane</location>
            <topology evidence="12">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="12">Bacterial flagellum basal body</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the FliP/MopC/SpaP family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12160.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5301"/>
    <dbReference type="EnsemblBacteria" id="ABF12160">
        <property type="protein sequence ID" value="ABF12160"/>
        <property type="gene ID" value="Rmet_5301"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5301"/>
    <dbReference type="eggNOG" id="COG1338">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_042028_0_1_4"/>
    <dbReference type="OMA" id="MPPNQIL"/>
    <dbReference type="OrthoDB" id="1049349at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009425">
        <property type="term" value="C:bacterial-type flagellum basal body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044781">
        <property type="term" value="P:bacterial-type flagellum organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009306">
        <property type="term" value="P:protein secretion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005837">
        <property type="entry name" value="FliP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005838">
        <property type="entry name" value="T3SS_IM_P"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30587">
        <property type="entry name" value="PTHR30587"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30587:SF0">
        <property type="entry name" value="PTHR30587:SF0"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00813">
        <property type="entry name" value="FliP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00951">
        <property type="entry name" value="FLGBIOSNFLIP"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01302">
        <property type="entry name" value="TYPE3IMPPROT"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01103">
        <property type="entry name" value="fliP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01060">
        <property type="entry name" value="FLIP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01061">
        <property type="entry name" value="FLIP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0975">Bacterial flagellum</keyword>
    <keyword evidence="7 12" id="KW-1005">Bacterial flagellum biogenesis</keyword>
    <keyword evidence="11 12" id="KW-1006">Bacterial flagellum protein export</keyword>
    <keyword evidence="5 12" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="13" id="KW-0966">Cell projection</keyword>
    <keyword evidence="13" id="KW-0969">Cilium</keyword>
    <keyword evidence="13" id="KW-0282">Flagellum</keyword>
    <keyword evidence="9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-0614">Plasmid</keyword>
    <keyword evidence="12" id="KW-0653">Protein transport</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4 12" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="63"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="105"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="204"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="242"/>
            <end position="263"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00021714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022795"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023143"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU362069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ABF12160.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="264" mass="28635" checksum="ED1987356B26E0A5" modified="2006-05-30" version="1">MNSLRADVARHPARLPRVAATLLALLPLVLWLAPESALAQSLPGVTSRPGPGGGQTWSLSIQTLVLLTSLSFLPAAMLMMTGFTRIIIVLGLLRNALGTASSPPNQVLVGLSLFLTFFVMSPVFDKVYTDAYQPLSENKISLETAAQRAAVPLRAFMLRQTREKDLAMFAQMAKTPELQGPEDVPFTVLVPAFVTSELKTAFQIGFTIFIPFLIIDLVVASVLMAMGMMMVPPATISLPFKLMLFVLVDGWQLLLGSLAQSFMN</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LCI4</accession>
    <name>Q1LCI4_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">6-aminohexanoate-dimer hydrolase</fullName>
            <ecNumber evidence="3">3.5.1.46</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">nylB</name>
        <name evidence="3" type="ordered locus">Rmet_5283</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EC" id="3.5.1.46" evidence="3"/>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12142.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519688.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5283"/>
    <dbReference type="EnsemblBacteria" id="ABF12142">
        <property type="protein sequence ID" value="ABF12142"/>
        <property type="gene ID" value="Rmet_5283"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5283"/>
    <dbReference type="eggNOG" id="COG1680">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030169_0_1_4"/>
    <dbReference type="OMA" id="HIWASMT"/>
    <dbReference type="OrthoDB" id="803444at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019875">
        <property type="term" value="F:6-aminohexanoate-dimer hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.710.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001466">
        <property type="entry name" value="Beta-lactam-related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012338">
        <property type="entry name" value="Beta-lactam/transpept-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00144">
        <property type="entry name" value="Beta-lactamase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56601">
        <property type="entry name" value="SSF56601"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193332" evidence="1">
        <location>
            <begin position="33"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="domain" description="Beta-lactamase" evidence="2">
        <location>
            <begin position="127"/>
            <end position="419"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF12142.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="450" mass="48516" checksum="5A31A3306E5D97F2" modified="2006-05-30" version="1">MDTVPRHRIHWQRKCRAIATIALLAVTGGVAAQSLGQSNDRKDVDVMEGFPPSPAMRVTKTNAFQPPYMRWAFRHARETSPTAGVRHASTPMTLPERPAASLDDVQFNVEGQSVKLSDYLRDTHTDGFIVLHKGQIVFERYLDGFGPAQPHIWASMTKSLTGLMAAMLIVDGRLNPEARLATYVPELVGNPFGDATVAQNLDMEVPVAYSPELPPDLGLFGAVGIIPRRAGAPDNIYDFLKTVSTDKRAVTGDYWFYQNGSPEAVAWAMRRISGKSWAELAQETVWSKLAQDDAYIQVDSLGTEMASGGLSTTLRDAARFADAVRRAQAGDADAGIPVQAVRAALHPRGNQAGFATGRLAAGRSGYAYGDYWYQVNDGDGSVEASGRFGQKIYINPKKELSVVKFSSSPDSAPRGASAEAGKTVAARNGSLESSTAFIAAIRAVYGAIPH</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LCJ0</accession>
    <name>Q1LCJ0_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Putative threonine efflux protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Rmet_5277</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="7">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="7">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12136.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519682.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF12136">
        <property type="protein sequence ID" value="ABF12136"/>
        <property type="gene ID" value="Rmet_5277"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5277"/>
    <dbReference type="eggNOG" id="COG1280">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_087840_1_1_4"/>
    <dbReference type="OMA" id="CIRRTLT"/>
    <dbReference type="OrthoDB" id="1953934at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006865">
        <property type="term" value="P:amino acid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001123">
        <property type="entry name" value="LeuE-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30086">
        <property type="entry name" value="PTHR30086"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01810">
        <property type="entry name" value="LysE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0614">Plasmid</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="42"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="75"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="113"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="147"/>
            <end position="170"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABF12136.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="205" mass="21283" checksum="5B844859DEB2DD80" modified="2006-05-30" version="1">MSLPALFLTAAGVGLAVAAPVGPMGMLCIRRTLTDGPRAGLAIGFGIACGDAIYGLVAALGLVGVSQFMLAYDKPLHLAAGLFLVYLGLRTFLQKPTDQAATLNSNGYGARAFGSALLLTLTNPQTIIMFAALFATLAPRGAFSTPIAMTTVLGVFCGSILWWCVLVTIVSGARHALGMRVRQWIDRAAGVALAAFGVSEIRRAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-10-07" version="50" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LCK7</accession>
    <name>Q1LCK7_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_5260</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12119.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5260"/>
    <dbReference type="EnsemblBacteria" id="ABF12119">
        <property type="protein sequence ID" value="ABF12119"/>
        <property type="gene ID" value="Rmet_5260"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5260"/>
    <dbReference type="HOGENOM" id="CLU_2452429_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="66"/>
            <end position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF12119.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="89" mass="9924" checksum="3F6E8340C769779D" modified="2006-05-30" version="1">MPAKARNRRENLPYSADCRWPFCPIIATVPDLAEQPRPLQPAPGHEHQLSRGVRVFFLQRDLSRAGKINGSKGQSGKGEFCLAKPETRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-10-07" version="52" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LCU0</accession>
    <name>Q1LCU0_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_5177</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12036.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF12036">
        <property type="protein sequence ID" value="ABF12036"/>
        <property type="gene ID" value="Rmet_5177"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5177"/>
    <dbReference type="HOGENOM" id="CLU_2013388_0_0_4"/>
    <dbReference type="OrthoDB" id="2065458at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018691">
        <property type="entry name" value="DUF2188"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09954">
        <property type="entry name" value="DUF2188"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF12036.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="123" mass="13366" checksum="803CC2604E5E0932" modified="2006-05-30" version="1">MCWIARNVADGQLSMRNGGALPVVGQRLIAETHLATRDCQYSAISLGAIMASRNIHVMPDDDRGWIVVVEGPNGATVLFPTEVEAIAAATDAAKQDGVKLFIHDQDGQIKERKSFDPLNHRKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="58" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LCU3</accession>
    <name>Q1LCU3_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_5174</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12033.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519579.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF12033">
        <property type="protein sequence ID" value="ABF12033"/>
        <property type="gene ID" value="Rmet_5174"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5174"/>
    <dbReference type="HOGENOM" id="CLU_1804613_0_0_4"/>
    <dbReference type="OMA" id="EQPNDPH"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193010" evidence="1">
        <location>
            <begin position="22"/>
            <end position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF12033.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="143" mass="15717" checksum="D59AD66C9763EC04" modified="2006-05-30" version="1">MALIRAALLGMMLTTASSAHAIRCEGSGKTLYTQDSACPDGYGIVDGSHRGSVSMLGKSEHVRENEATFLSDRALERETMRGHEARIKMASGVNQSRAEACEALSYQARSVDAAMEQPNDPHALNTLRKLHRNIREEQQRNDC</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LCU7</accession>
    <name>Q1LCU7_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">NUDIX hydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_5170</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12029.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519574.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5170"/>
    <dbReference type="EnsemblBacteria" id="ABF12029">
        <property type="protein sequence ID" value="ABF12029"/>
        <property type="gene ID" value="Rmet_5170"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5170"/>
    <dbReference type="eggNOG" id="COG1051">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037162_25_1_4"/>
    <dbReference type="OMA" id="RATVICK"/>
    <dbReference type="OrthoDB" id="1846332at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015797">
        <property type="entry name" value="NUDIX_hydrolase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020084">
        <property type="entry name" value="NUDIX_hydrolase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000086">
        <property type="entry name" value="NUDIX_hydrolase_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00293">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55811">
        <property type="entry name" value="SSF55811"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51462">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00893">
        <property type="entry name" value="NUDIX_BOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nudix hydrolase" evidence="2">
        <location>
            <begin position="1"/>
            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF12029.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="124" mass="13596" checksum="19B340215ABFC30A" modified="2006-05-30" version="1">MKTKVRATVVCARGDRVLLVSKDGSRWALPGGRPTKNETFADAAGRELLEETTLLARGLGFLFQVVGATTVHHVFVANIGKSAVPKPSKEIQGCQWFSQAELGKIIISPTTRHIIESVWAERQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="117" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LCZ0</accession>
    <name>Q1LCZ0_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Putative sigma-54-dependent transcriptional regulator, HTH Fis-type family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Rmet_5127</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="9">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="9">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11986.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519533.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5127"/>
    <dbReference type="EnsemblBacteria" id="ABF11986">
        <property type="protein sequence ID" value="ABF11986"/>
        <property type="gene ID" value="Rmet_5127"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5127"/>
    <dbReference type="eggNOG" id="COG3284">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_8_12_4"/>
    <dbReference type="OMA" id="QFLTCSC"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003018">
        <property type="entry name" value="GAF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029016">
        <property type="entry name" value="GAF-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002197">
        <property type="entry name" value="HTH_Fis"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002078">
        <property type="entry name" value="Sigma_54_int"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025662">
        <property type="entry name" value="Sigma_54_int_dom_ATP-bd_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025943">
        <property type="entry name" value="Sigma_54_int_dom_ATP-bd_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025944">
        <property type="entry name" value="Sigma_54_int_dom_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01590">
        <property type="entry name" value="GAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02954">
        <property type="entry name" value="HTH_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00158">
        <property type="entry name" value="Sigma54_activat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01590">
        <property type="entry name" value="HTHFIS"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00675">
        <property type="entry name" value="SIGMA54_INTERACT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00676">
        <property type="entry name" value="SIGMA54_INTERACT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00688">
        <property type="entry name" value="SIGMA54_INTERACT_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50045">
        <property type="entry name" value="SIGMA54_INTERACT_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0614">Plasmid</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Sigma-54 factor interaction" evidence="7">
        <location>
            <begin position="342"/>
            <end position="567"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="312"/>
            <end position="341"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50045"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ABF11986.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="654" mass="70908" checksum="C55B10C298E09248" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="95" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LD13</accession>
    <name>Q1LD13_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Nicotinamide-nucleotide adenylyltransferase</fullName>
            <ecNumber evidence="4">2.7.7.1</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Rmet_5104</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="5">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="5">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the Nudix hydrolase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.1" evidence="4"/>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11963.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519510.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5104"/>
    <dbReference type="EnsemblBacteria" id="ABF11963">
        <property type="protein sequence ID" value="ABF11963"/>
        <property type="gene ID" value="Rmet_5104"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5104"/>
    <dbReference type="eggNOG" id="COG1051">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG1056">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_068406_0_0_4"/>
    <dbReference type="OMA" id="YPPVFVT"/>
    <dbReference type="OrthoDB" id="1846332at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000309">
        <property type="term" value="F:nicotinamide-nucleotide adenylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009058">
        <property type="term" value="P:biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004821">
        <property type="entry name" value="Cyt_trans-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020476">
        <property type="entry name" value="Nudix_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015797">
        <property type="entry name" value="NUDIX_hydrolase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020084">
        <property type="entry name" value="NUDIX_hydrolase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000086">
        <property type="entry name" value="NUDIX_hydrolase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01467">
        <property type="entry name" value="CTP_transf_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00293">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00502">
        <property type="entry name" value="NUDIXFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55811">
        <property type="entry name" value="SSF55811"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00125">
        <property type="entry name" value="cyt_tran_rel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51462">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00893">
        <property type="entry name" value="NUDIX_BOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="4" id="KW-0614">Plasmid</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Nudix hydrolase" evidence="3">
        <location>
            <begin position="205"/>
            <end position="345"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU003476"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF11963.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="345" mass="39414" checksum="909AF4D6C37528BF" modified="2006-05-30" version="1">MSTNTKRFDALVFIGRFQPMHRGHVDVLRRALSQADTVCILIGSTDKPRTIKDPFSFDERRQMIESVLDESSRARVRIGAVQDSTYNDSDWVRWVQDAVAGLLGDTAGRRIGIIGHEKDGSSYYLRMFPQWELVEADSTEDISATEIREQFLAERSNSFVSWAVPAPVFQWMELFRDRPEFAQLKAEAEFIAGYRKAWSAAPYPVTFVTVDAVVVHSGHLLLVRRRSEPGRGLWALPGGFVNQDERLETACLRELREETGLKLPEPVLRGSLKDRQVFDHPQRSLRGRTITHAFLFSFPVGELPRVKGGDDADKARWVPLNDFARMRSVMFEDHFDIAYHFLGKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="64" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LD58</accession>
    <name>Q1LD58_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_5056</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11918.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5056"/>
    <dbReference type="EnsemblBacteria" id="ABF11918">
        <property type="protein sequence ID" value="ABF11918"/>
        <property type="gene ID" value="Rmet_5056"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5056"/>
    <dbReference type="eggNOG" id="COG0446">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1794876_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021095">
        <property type="entry name" value="DUF3734"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023753">
        <property type="entry name" value="FAD/NAD-binding_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12536">
        <property type="entry name" value="DUF3734"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07992">
        <property type="entry name" value="Pyr_redox_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="SSF51905"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pyr_redox_2" evidence="1">
        <location>
            <begin position="4"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="domain" description="DUF3734" evidence="2">
        <location>
            <begin position="81"/>
            <end position="113"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF07992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF11918.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="144" mass="15212" checksum="F9562C1D0E9D1A63" modified="2006-05-30" version="1">MVVALHGQRKAPEVETRSGRRPPCDVVVVCAGVDPATRFLKGSGIALEKGDVAVDELLQTNVPNVWAAGDVTSFRDPVFTRRSDEPSSRDYDFSKTAIQANLAQGYAEVTVVLGPISNASDGVVVRLGDSDESVTGDVPDGVMP</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LDC5</accession>
    <name>Q1LDC5_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Putative endonuclease/exonuclease/phosphatase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Rmet_4989</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="5">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="5">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11851.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519399.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4989"/>
    <dbReference type="EnsemblBacteria" id="ABF11851">
        <property type="protein sequence ID" value="ABF11851"/>
        <property type="gene ID" value="Rmet_4989"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4989"/>
    <dbReference type="eggNOG" id="COG2374">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006338_0_1_4"/>
    <dbReference type="OMA" id="VEWHINA"/>
    <dbReference type="OrthoDB" id="1884225at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004527">
        <property type="term" value="F:exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005135">
        <property type="entry name" value="Endo/exonuclease/phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001322">
        <property type="entry name" value="Lamin_tail_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03372">
        <property type="entry name" value="Exo_endo_phos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00932">
        <property type="entry name" value="LTD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="SSF56219"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51841">
        <property type="entry name" value="LTD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="4" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0540">Nuclease</keyword>
    <keyword evidence="4" id="KW-0614">Plasmid</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193085" evidence="2">
        <location>
            <begin position="20"/>
            <end position="848"/>
        </location>
    </feature>
    <feature type="domain" description="LTD" evidence="3">
        <location>
            <begin position="9"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="763"/>
            <end position="818"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="783"/>
            <end position="818"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51841"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF11851.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="848" mass="87901" checksum="D635648E7A06FC11" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="69" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LDK3</accession>
    <name>Q1LDK3_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_4911</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11773.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519332.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4911"/>
    <dbReference type="EnsemblBacteria" id="ABF11773">
        <property type="protein sequence ID" value="ABF11773"/>
        <property type="gene ID" value="Rmet_4911"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4911"/>
    <dbReference type="eggNOG" id="COG4314">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1365272_0_0_4"/>
    <dbReference type="OMA" id="GPMGHEF"/>
    <dbReference type="OrthoDB" id="1678497at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008719">
        <property type="entry name" value="N2O_reductase_NosL"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR41247">
        <property type="entry name" value="PTHR41247"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05573">
        <property type="entry name" value="NosL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF11773.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="181" mass="19644" checksum="805CF0EED8F8307D" modified="2006-05-30" version="1">MGWYAFHARVRASSQVPTDICFVAPATPYDPASGIAPDAPRVVPTNARCPVCGAFPSRVPEWAAQVIFSDGDAYFFDSPLSLFIYLRNLPRYAPSRTASEIAASYVRATEGGQWIATNEAVYVQGSSVPGPMRQGNLPAFEDVMAARHFISRHGGTVLHSPDITVQLLQDLAPTMHRADHA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LDP7</accession>
    <name>Q1LDP7_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Multidrug transport protein, cation/multidrug efflux system, RND family efflux transporter MFP (Membrane-fusion component)subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">acrA</name>
        <name evidence="4" type="ordered locus">Rmet_4867</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="5">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="5">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11729.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519289.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4867"/>
    <dbReference type="EnsemblBacteria" id="ABF11729">
        <property type="protein sequence ID" value="ABF11729"/>
        <property type="gene ID" value="Rmet_4867"/>
    </dbReference>
    <dbReference type="GeneID" id="60823880"/>
    <dbReference type="KEGG" id="rme:Rmet_4867"/>
    <dbReference type="eggNOG" id="COG0845">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018816_2_1_4"/>
    <dbReference type="OMA" id="RASILIK"/>
    <dbReference type="OrthoDB" id="1532186at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032317">
        <property type="entry name" value="HlyD_D23"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006143">
        <property type="entry name" value="RND_pump_MFP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006311">
        <property type="entry name" value="TAT_signal"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16576">
        <property type="entry name" value="HlyD_D23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01730">
        <property type="entry name" value="RND_mfp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51318">
        <property type="entry name" value="TAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0614">Plasmid</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HlyD_D23" evidence="3">
        <location>
            <begin position="72"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="383"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="390"/>
            <end position="411"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF16576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF11729.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="411" mass="43927" checksum="7AE44BD2C5C6E629" modified="2006-05-30" version="1">MNQQSRRTLIAIAIVAILGTGVATSVLRPWHGGEAQANTPPPAPAVEVSAAVGKTITEWDEFSGRIEAVDRVEIRPRVSGTIETVHFREGSIVKKGDPLFTIDPRPYAAEVARAEAALAAAQVRASLAQTEKARAQRLLDDNAISRREFDERINAASEMSADVRGAQAALEVAKLNLSYTRIVAPVSGKVSRAEITVGNLVAAGAASPALTTVVSVSPVYASFDVDEQSYLRYTAPGASGGKNDLPVFLGLANEDNTPHEGKIHSVDNRLDTRSGTIRVRAIFANEDGRLLPGLYAKIKLGGGTPHPAVLINDRAIGTDQGKKFVLVVDQANKLTYREVELGPTYEGLRVIRKGLKPGESIVVNGLMRVRPGDTVAPKPVSMAFRSELEPRGNAPDRKQAAEEKAEKPNVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-12-02" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LDP8</accession>
    <name>Q1LDP8_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Efflux pump membrane transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">acrB</name>
        <name evidence="6" type="ordered locus">Rmet_4866</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="7">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="7">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 4">Cell inner membrane</location>
            <topology evidence="2 4">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 4">Belongs to the resistance-nodulation-cell division (RND) (TC 2.A.6) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11728.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4866"/>
    <dbReference type="EnsemblBacteria" id="ABF11728">
        <property type="protein sequence ID" value="ABF11728"/>
        <property type="gene ID" value="Rmet_4866"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4866"/>
    <dbReference type="eggNOG" id="COG0841">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002755_1_2_4"/>
    <dbReference type="OMA" id="IMIVPMG"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015562">
        <property type="term" value="F:efflux transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042908">
        <property type="term" value="P:xenobiotic transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2090.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027463">
        <property type="entry name" value="AcrB_DN_DC_subdom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001036">
        <property type="entry name" value="Acrflvin-R"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004764">
        <property type="entry name" value="HAE1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000731">
        <property type="entry name" value="SSD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32063">
        <property type="entry name" value="PTHR32063"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00873">
        <property type="entry name" value="ACR_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00702">
        <property type="entry name" value="ACRIFLAVINRP"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82714">
        <property type="entry name" value="SSF82714"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00915">
        <property type="entry name" value="2A0602"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50156">
        <property type="entry name" value="SSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0614">Plasmid</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="29"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="360"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="387"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="413"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="458"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="490"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="565"/>
            <end position="585"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="898"/>
            <end position="916"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="923"/>
            <end position="945"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="951"/>
            <end position="974"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="995"/>
            <end position="1016"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="1028"/>
            <end position="1054"/>
        </location>
    </feature>
    <feature type="domain" description="SSD" evidence="5">
        <location>
            <begin position="386"/>
            <end position="515"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004429"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU364070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABF11728.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="1081" mass="117259" checksum="17903C521EE3911D" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LDY1</accession>
    <name>Q1LDY1_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative transporter, major facilitator superfamily (MFS)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_4783</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11645.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519213.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4783"/>
    <dbReference type="EnsemblBacteria" id="ABF11645">
        <property type="protein sequence ID" value="ABF11645"/>
        <property type="gene ID" value="Rmet_4783"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4783"/>
    <dbReference type="eggNOG" id="COG3202">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027240_1_0_4"/>
    <dbReference type="OMA" id="WLFTGTF"/>
    <dbReference type="OrthoDB" id="1046620at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="SSF103473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="26"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="60"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="90"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="121"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="153"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="181"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="244"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="284"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="314"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="404"/>
            <end position="424"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF11645.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="453" mass="48643" checksum="F6D06D4C99ED4E4D" modified="2006-05-30" version="1">MDTPDLPPNPAGPSGLLGIDRSETPAVVAGFLFFFCLFASYFMLRPVRETMGIAGGVQNLQWLFTATFVVMLVAIPFYGACCKWLPRRRFVPWVYAFFIANLVAFALVTRALPDSVWLARVFYVWISVFNLFVVSVAWSLMADVFRPEQAKRLFAIIAAGASAGGLAGPVLGAGLVGHIGLTGLMLLSAALLAATLPGVGYLFAWRRRYGAGAPGSETASAADPAQPVGGGLWAGLSLVLRSRYLIGISLFVILLATSSTFLYFEQARLVAATFHSRAQQTQVFSALDATVQALTILVQIFFSGRVARRYGVTALLTAVPLAVTGGFLLLAMWPTFGVLAAAMIVRRVGEYAMLRPGREMLFTVVDPETKYKAKNVIDTAIYRAGDAVSAWVKTAIDALSGHPATVALAGAVLAIVWGWLGWWLGRQHEGQLTELTVEKSSPAVLARGQTGSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="69" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LE77</accession>
    <name>Q1LE77_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_4687</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11549.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519121.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4687"/>
    <dbReference type="EnsemblBacteria" id="ABF11549">
        <property type="protein sequence ID" value="ABF11549"/>
        <property type="gene ID" value="Rmet_4687"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4687"/>
    <dbReference type="eggNOG" id="COG4944">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_097826_1_0_4"/>
    <dbReference type="OMA" id="RTWRTCA"/>
    <dbReference type="OrthoDB" id="1539086at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009495">
        <property type="entry name" value="NrsF"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06532">
        <property type="entry name" value="NrsF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="25"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="59"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="90"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="131"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="156"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="188"/>
            <end position="207"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF11549.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="213" mass="23057" checksum="C65FB83523AF496E" modified="2006-05-30" version="1">MKTDDLISLLATGIRPVDPHIMHRAFVRALCAGAAGALLLLTMTYGVRPDIRVMLVTPIFWLKVAFPLSLAIGAFVVLKRLVVPGAQVGTRWAVPGVPVLAVWIGALAVLANVPATERLSLMLGMTWRTCPFNIVFLSLPLCVALVWAVRQMAPTNLRAASALAGLLAGSVATIVYCLHCPEMEVPFWAIWYLAGMLIPAALGWLLGPRLLRW</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="52" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LE83</accession>
    <name>Q1LE83_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_4681</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11543.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4681"/>
    <dbReference type="EnsemblBacteria" id="ABF11543">
        <property type="protein sequence ID" value="ABF11543"/>
        <property type="gene ID" value="Rmet_4681"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4681"/>
    <dbReference type="HOGENOM" id="CLU_2481056_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="53"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="53"/>
            <end position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF11543.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="87" mass="9595" checksum="202F13616DCFB319" modified="2006-05-30" version="1">MRKSQLSDAALQRDMLCVTQGFWPCKYASNRIAQCSIDVRTDGLDLADIRTEPPCRKENAPANERAGAEGEDARGFSGQVEVHLRAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-12-02" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LEA0</accession>
    <name>Q1LEA0_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Phosphoryl transfer system, HPr</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_4664</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11526.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4664"/>
    <dbReference type="EnsemblBacteria" id="ABF11526">
        <property type="protein sequence ID" value="ABF11526"/>
        <property type="gene ID" value="Rmet_4664"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4664"/>
    <dbReference type="eggNOG" id="COG1925">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_136230_5_1_4"/>
    <dbReference type="OMA" id="GPWVANA"/>
    <dbReference type="OrthoDB" id="2083061at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="CDD" id="cd00367">
        <property type="entry name" value="PTS-HPr_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1340.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000032">
        <property type="entry name" value="HPr-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035895">
        <property type="entry name" value="HPr-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00381">
        <property type="entry name" value="PTS-HPr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00107">
        <property type="entry name" value="PHOSPHOCPHPR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55594">
        <property type="entry name" value="SSF55594"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01003">
        <property type="entry name" value="PTS_HPr_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51350">
        <property type="entry name" value="PTS_HPR_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HPr" evidence="1">
        <location>
            <begin position="1"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51350"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF11526.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="83" mass="8738" checksum="585D2ED8672CD700" modified="2006-05-30" version="1">MVEKSIVVTSSIGLQGRGSARLAHAANQFESDLLLVNDVCIVNAKDVMQVLRFSARAGSRVRVQASGSDEAAAVKRLVALLTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LEP9</accession>
    <name>Q1LEP9_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Protein CyaE</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Rmet_4512</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="6">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="6">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">CyaE is necessary for transport of calmodulin-sensitive adenylate cyclase-hemolysin (cyclolysin).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell outer membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the outer membrane factor (OMF) (TC 1.B.17) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11377.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4512"/>
    <dbReference type="EnsemblBacteria" id="ABF11377">
        <property type="protein sequence ID" value="ABF11377"/>
        <property type="gene ID" value="Rmet_4512"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4512"/>
    <dbReference type="eggNOG" id="COG1538">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012817_10_2_4"/>
    <dbReference type="OMA" id="EAAFMPK"/>
    <dbReference type="OrthoDB" id="1099555at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009279">
        <property type="term" value="C:cell outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015562">
        <property type="term" value="F:efflux transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044179">
        <property type="term" value="P:hemolysis in another organism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028351">
        <property type="entry name" value="CyaE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003423">
        <property type="entry name" value="OMP_efflux"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02321">
        <property type="entry name" value="OEP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001892">
        <property type="entry name" value="CyaE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0998">Cell outer membrane</keyword>
    <keyword evidence="2" id="KW-0204">Cytolysis</keyword>
    <keyword evidence="2" id="KW-0354">Hemolysis</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0614">Plasmid</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193430" description="Protein CyaE" evidence="4">
        <location>
            <begin position="25"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="29"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRNR" id="PIRNR001892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABF11377.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="520" mass="53533" checksum="43DF1AB0E52F216C" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="70" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LF64</accession>
    <name>Q1LF64_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Alkylhydroperoxidase AhpD core</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_4347</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11212.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF11212">
        <property type="protein sequence ID" value="ABF11212"/>
        <property type="gene ID" value="Rmet_4347"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4347"/>
    <dbReference type="eggNOG" id="COG0599">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1577221_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004601">
        <property type="term" value="F:peroxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051920">
        <property type="term" value="F:peroxiredoxin activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1290.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029032">
        <property type="entry name" value="AhpD-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004675">
        <property type="entry name" value="AhpD_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003779">
        <property type="entry name" value="CMD-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02627">
        <property type="entry name" value="CMD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69118">
        <property type="entry name" value="SSF69118"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00778">
        <property type="entry name" value="ahpD_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="2" id="KW-0575">Peroxidase</keyword>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CMD" evidence="1">
        <location>
            <begin position="95"/>
            <end position="152"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02627"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF11212.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="169" mass="17803" checksum="981CC1FA3A2EDADD" modified="2006-05-30" version="1">MDWKPLGGRHCGAQTEPIDSPGASVSRSVALQYYLGRSVMSVMRTLLASAGLALLLALATEPSVSAQEAPEWMKQTLPEQALKPHWDESRAVMNPTGALDAKTKQLIALGVAAQIPCAYCVSAHTKAAKAAGATDAQIKEAIATAALVRFNSTMLNGSDGGTEWSPQRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LFL7</accession>
    <name>Q1LFL7_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Phospholipase C</fullName>
            <ecNumber evidence="2">3.1.4.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">plcN</name>
        <name evidence="6" type="ordered locus">Rmet_4192</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="7">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="7">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the bacterial phospholipase C family.</text>
    </comment>
    <dbReference type="EC" id="3.1.4.3" evidence="2"/>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11059.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518681.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4192"/>
    <dbReference type="EnsemblBacteria" id="ABF11059">
        <property type="protein sequence ID" value="ABF11059"/>
        <property type="gene ID" value="Rmet_4192"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4192"/>
    <dbReference type="eggNOG" id="COG3511">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008770_1_0_4"/>
    <dbReference type="OMA" id="QGAWYTQ"/>
    <dbReference type="OrthoDB" id="250274at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034480">
        <property type="term" value="F:phosphatidylcholine phospholipase C activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.720.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017850">
        <property type="entry name" value="Alkaline_phosphatase_core_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017767">
        <property type="entry name" value="Bact_PC-PLC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007312">
        <property type="entry name" value="Phosphoesterase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008475">
        <property type="entry name" value="PLipase_C_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006311">
        <property type="entry name" value="TAT_signal"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019546">
        <property type="entry name" value="TAT_signal_bac_arc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31956">
        <property type="entry name" value="PTHR31956"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05506">
        <property type="entry name" value="DUF756"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04185">
        <property type="entry name" value="Phosphoesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03396">
        <property type="entry name" value="PC_PLC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01409">
        <property type="entry name" value="TAT_signal_seq"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51318">
        <property type="entry name" value="TAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0614">Plasmid</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193096" description="Phospholipase C" evidence="4">
        <location>
            <begin position="25"/>
            <end position="714"/>
        </location>
    </feature>
    <feature type="domain" description="DUF756" evidence="5">
        <location>
            <begin position="517"/>
            <end position="605"/>
        </location>
    </feature>
    <feature type="domain" description="DUF756" evidence="5">
        <location>
            <begin position="618"/>
            <end position="699"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="499"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="503"/>
            <end position="518"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009717"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF05506"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABF11059.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="714" mass="77823" checksum="30E7144C51D13D08" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="68" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LFX1</accession>
    <name>Q1LFX1_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Paraquat-inducible protein B</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">pqiB</name>
        <name evidence="2" type="ordered locus">Rmet_4088</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10955.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4088"/>
    <dbReference type="EnsemblBacteria" id="ABF10955">
        <property type="protein sequence ID" value="ABF10955"/>
        <property type="gene ID" value="Rmet_4088"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4088"/>
    <dbReference type="eggNOG" id="COG1463">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG3008">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018765_3_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003399">
        <property type="entry name" value="Mce/MlaD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02470">
        <property type="entry name" value="MlaD"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MlaD" evidence="1">
        <location>
            <begin position="21"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="domain" description="MlaD" evidence="1">
        <location>
            <begin position="136"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="domain" description="MlaD" evidence="1">
        <location>
            <begin position="266"/>
            <end position="370"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02470"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF10955.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="508" mass="54823" checksum="F9B95F96B095761B" modified="2010-03-23" version="2">MPIIAVLIGGWLAVKAVIEQGPTITVSFKTGEGLEAGKTKIKFKDVDIGLVKSVALSPDSKRVVVTAEIAKDAGRLLVDNTRFWVVRPRISGGSVSGLGTLLSGSYIATDAGTATATRHDFVGLETPPAFTSDVPGREFTLKSEDVGSLDVGSPVYFRRLQVGQITAFDLDSDGRGVTLKVFVNAPYDRFVQQGTRFWHASGVDVSLDTGGLRVNTQSVVSILIGGIGFETPYESQDQPVAAAGSTFALFHDRVTALKRQDSVVHHYVVNFTDTVRGLAVGAPIEFRGIVIGEVTGIYTRYDPVTRKFSIPVEINFYPGRFTSRYRTGAGAGPVTETPRELGDFLVSQGLRMQLRNGNLLTGQLYIALDFFPNAPKAKINWNENPPEFPSIQGDLQSLQQSLTRVVAQLNSVPFAQIGQNTKDMLRNATSLLTRLDSELVPQARDTLASARSTLDSANTALGPDSALQQNTADAMKELARTAAALRSLADYLEQHPEALIRGKAEDKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LGC2</accession>
    <name>Q1LGC2_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Putative drug resistance transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Rmet_3936</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="9">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="9">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10804.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3936"/>
    <dbReference type="EnsemblBacteria" id="ABF10804">
        <property type="protein sequence ID" value="ABF10804"/>
        <property type="gene ID" value="Rmet_3936"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3936"/>
    <dbReference type="eggNOG" id="COG0477">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000960_2_5_4"/>
    <dbReference type="OMA" id="GFGMSPK"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
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    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="SSF103473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0614">Plasmid</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="38"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="74"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="105"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="162"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="194"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="253"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="295"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="331"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="361"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="388"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="432"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="507"/>
            <end position="529"/>
        </location>
    </feature>
    <feature type="domain" description="MFS" evidence="7">
        <location>
            <begin position="40"/>
            <end position="521"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ABF10804.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="548" mass="58418" checksum="AE0E7497F7B27372" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LGM4</accession>
    <name>Q1LGM4_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Extra-cytoplasmic solute receptor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">bugT</name>
        <name evidence="4" type="ordered locus">Rmet_3832</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="5">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="5">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Periplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the UPF0065 (bug) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10702.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518348.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3832"/>
    <dbReference type="EnsemblBacteria" id="ABF10702">
        <property type="protein sequence ID" value="ABF10702"/>
        <property type="gene ID" value="Rmet_3832"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3832"/>
    <dbReference type="eggNOG" id="COG3181">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045683_0_0_4"/>
    <dbReference type="OMA" id="GYNGTLW"/>
    <dbReference type="OrthoDB" id="447734at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042597">
        <property type="term" value="C:periplasmic space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005064">
        <property type="entry name" value="BUG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042100">
        <property type="entry name" value="Bug_dom1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42928">
        <property type="entry name" value="PTHR42928"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03401">
        <property type="entry name" value="TctC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017082">
        <property type="entry name" value="YflP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-0675">Receptor</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193159" evidence="3">
        <location>
            <begin position="24"/>
            <end position="322"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF10702.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="322" mass="33816" checksum="0F72ADBA6896785D" modified="2006-05-30" version="1">MLRTIRAIAGIAMALSVVGHAQADTYPSKPIQMIVPQAPGGTNDIVARLVAADLSQRLGQQVVVENRPGAGGNIGTQTAARSAPDGYTLLMTISSTQAINPSLYRSIPFDPIKDFEPIAPVATVPNVLVVNPAFPAKSMSELIAMAKAKPDYYRFASAGNGTLNHLLGEMLNSMAAIKLEHVPYKGVAPALNDVLGNQVPMAFASLPSVLAHIKAGKVRALGVSSAKRSPFAPDIPAISETVPGYSGDLWVGLFAVRGTPKDVTQKLAATMQTALGDKTLRDKLAAQGAEVLTGTPQQFSTMLRGDIDKWAKIVKQSGAQVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LGP4</accession>
    <name>Q1LGP4_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative dehydratase (MaoC-like domain)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_3812</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10682.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008651486.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3812"/>
    <dbReference type="EnsemblBacteria" id="ABF10682">
        <property type="protein sequence ID" value="ABF10682"/>
        <property type="gene ID" value="Rmet_3812"/>
    </dbReference>
    <dbReference type="GeneID" id="60824329"/>
    <dbReference type="KEGG" id="rme:Rmet_3812"/>
    <dbReference type="eggNOG" id="COG2030">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_094876_0_0_4"/>
    <dbReference type="OMA" id="CRAHGMP"/>
    <dbReference type="OrthoDB" id="1563802at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029069">
        <property type="entry name" value="HotDog_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002539">
        <property type="entry name" value="MaoC-like_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01575">
        <property type="entry name" value="MaoC_dehydratas"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54637">
        <property type="entry name" value="SSF54637"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MaoC-like" evidence="1">
        <location>
            <begin position="17"/>
            <end position="124"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01575"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF10682.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="156" mass="17677" checksum="5BF8E1636517BC6B" modified="2006-05-30" version="1">MIRHELCAQRYFEDFEVGERMNLPSRTMTDALFAAFQLASGDNHPVHYDVEYCRAHGMPHMLAHGFQVLIQTAAGAGLFPHMVEESLKGFIEQSSRFLAPVYVGDTLYCSLTVSELIPGRTTGVLVMQSEIRNQNDVRVMEGTQKYLLRKRVPDAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="99" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LGU5</accession>
    <name>Q1LGU5_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 5">Glutaminase</fullName>
            <ecNumber evidence="3 5">3.5.1.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">glsA</name>
        <name evidence="6" type="ordered locus">Rmet_3760</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="7">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="7">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 5">
            <text>H2O + L-glutamine = L-glutamate + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:15889"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="EC" id="3.5.1.2"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="5">Homotetramer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the glutaminase family.</text>
    </comment>
    <dbReference type="EC" id="3.5.1.2" evidence="3 5"/>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10631.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518277.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3760"/>
    <dbReference type="EnsemblBacteria" id="ABF10631">
        <property type="protein sequence ID" value="ABF10631"/>
        <property type="gene ID" value="Rmet_3760"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3760"/>
    <dbReference type="eggNOG" id="COG2066">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027932_1_0_4"/>
    <dbReference type="OMA" id="MYTCGMY"/>
    <dbReference type="OrthoDB" id="1288854at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004359">
        <property type="term" value="F:glutaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006541">
        <property type="term" value="P:glutamine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.710.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00313">
        <property type="entry name" value="Glutaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012338">
        <property type="entry name" value="Beta-lactam/transpept-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015868">
        <property type="entry name" value="Glutaminase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12544">
        <property type="entry name" value="PTHR12544"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04960">
        <property type="entry name" value="Glutaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56601">
        <property type="entry name" value="SSF56601"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03814">
        <property type="entry name" value="Gln_ase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0007">Acetylation</keyword>
    <keyword evidence="4 5 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0614">Plasmid</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="5">
        <location>
            <position position="82"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="133"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="177"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="184"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="208"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="260"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="278"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001062"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00313"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABF10631.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="332" mass="34575" checksum="AED79559AC25F979" modified="2006-05-30" version="1">MNSSSTPTARFVSTGTLPGPEEIRSLVEAAYENFREEAGGKLADYIPALAEADPSAFGICVANTRGHAFMVGEAAETFSIQSVSKPFVFALVCEALGAAEAATRLGVDATGLPFNSIMAMELRTDGTSNPLVNAGAIAATSLIPGFGADDRWHHVRQGLSRFAGRELACDEHVYESEAANNQRNAGLAHLLRGHGRLYCDPDEAVEVYTRQCALLVSARDLALMGATLAGGGVHPLTGEQVVSAATCQRVLAVMATAGLYERSGSWLYDVGLPGKSGVSGGILTIAPGKGAVATFSPPLDAAGNSVRGQLVMRFLSERLGLNLFISAPAAHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="102" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LH35</accession>
    <name>Q1LH35_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">3-oxoadipyl-CoA thiolase</fullName>
            <ecNumber evidence="4">2.3.1.174</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">Rmet_3669</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="10">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="10">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>acetyl-CoA + succinyl-CoA = 3-oxoadipyl-CoA + CoA</text>
            <dbReference type="Rhea" id="RHEA:19481"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:57292"/>
            <dbReference type="ChEBI" id="CHEBI:57348"/>
            <dbReference type="EC" id="2.3.1.174"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Aromatic compound metabolism.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 6">Belongs to the thiolase-like superfamily. Thiolase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.174" evidence="4"/>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10541.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008645349.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3669"/>
    <dbReference type="EnsemblBacteria" id="ABF10541">
        <property type="protein sequence ID" value="ABF10541"/>
        <property type="gene ID" value="Rmet_3669"/>
    </dbReference>
    <dbReference type="GeneID" id="60826224"/>
    <dbReference type="KEGG" id="rme:Rmet_3669"/>
    <dbReference type="eggNOG" id="COG0183">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031026_0_0_4"/>
    <dbReference type="OMA" id="NASPMND"/>
    <dbReference type="OrthoDB" id="550338at2"/>
    <dbReference type="UniPathway" id="UPA00554">
        <property type="reaction ID" value="UER00611"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033812">
        <property type="term" value="F:3-oxoadipyl-CoA thiolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019619">
        <property type="term" value="P:3,4-dihydroxybenzoate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00751">
        <property type="entry name" value="thiolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.47.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012793">
        <property type="entry name" value="PcaF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002155">
        <property type="entry name" value="Thiolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016039">
        <property type="entry name" value="Thiolase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020610">
        <property type="entry name" value="Thiolase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020617">
        <property type="entry name" value="Thiolase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020613">
        <property type="entry name" value="Thiolase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020616">
        <property type="entry name" value="Thiolase_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02803">
        <property type="entry name" value="Thiolase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00108">
        <property type="entry name" value="Thiolase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000429">
        <property type="entry name" value="Ac-CoA_Ac_transf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53901">
        <property type="entry name" value="SSF53901"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01930">
        <property type="entry name" value="AcCoA-C-Actrans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02430">
        <property type="entry name" value="pcaF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00737">
        <property type="entry name" value="THIOLASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00099">
        <property type="entry name" value="THIOLASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="9" id="KW-0614">Plasmid</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Thiolase_N" evidence="7">
        <location>
            <begin position="5"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="domain" description="Thiolase_C" evidence="8">
        <location>
            <begin position="275"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="active site" description="Acyl-thioester intermediate" evidence="5">
        <location>
            <position position="90"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="5">
        <location>
            <position position="356"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="5">
        <location>
            <position position="386"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012233"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR000429-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ABF10541.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="400" mass="41672" checksum="CD67E061A5551B3D" modified="2006-05-30" version="1">MSEAFIVDAIRTPIGRYAGSLSGVRADDLGAVPLKALMARNASVDWSAIDDVIFGNANQAGEDNRNVARMSALLAGLPDAVPGATINRLCGSGMDATGTAARAIKAGEANLMISGGVESMSRAPFVMGKATSAFSRDAQIFDTTIGWRFINPAMRAAYGVDSMPETAENVATDYKIGREDQDLMALRSQEKASRAQADGTLAQEITPVTIPQKKGDAIVVDRDEHPRATSMEALAKLRGVVRPDGTVTAGNASGVNDGACALLLASEAGLKQHGLKPRARIVGMATAGVAPRVMGIGPAPATQKLMKQLGMTLDQIDVIELNEAFAAQGLAVLRQLGIADDDKRVNPNGGAIALGHPLGMSGARLVTTAMYQLERTGGRFALCTMCIGVGQGIAMVIERV</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LH59</accession>
    <name>Q1LH59_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Carboxymethylenebutenolidase (Dienelactone) hydrolase (DLH)</fullName>
            <ecNumber evidence="2">3.1.1.45</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_3645</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EC" id="3.1.1.45" evidence="2"/>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10517.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518172.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3645"/>
    <dbReference type="ESTHER" id="ralme-q1lh59">
        <property type="family name" value="Dienelactone_hydrolase"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF10517">
        <property type="protein sequence ID" value="ABF10517"/>
        <property type="gene ID" value="Rmet_3645"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3645"/>
    <dbReference type="eggNOG" id="COG0412">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_054590_7_3_4"/>
    <dbReference type="OMA" id="FAFFGEH"/>
    <dbReference type="OrthoDB" id="1889569at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008806">
        <property type="term" value="F:carboxymethylenebutenolidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002925">
        <property type="entry name" value="Dienelactn_hydro"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01738">
        <property type="entry name" value="DLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="SSF53474"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DLH" evidence="1">
        <location>
            <begin position="17"/>
            <end position="233"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF10517.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="236" mass="25487" checksum="908E13746F8E196B" modified="2006-05-30" version="1">MTTNSRWIRIDTPDGSFDAYLSLPPAGKQPGNPGIVLIQEIFGVNEHIRSVADQYAADGYVVLAPDVFWRTAPRVELGYSGKDWEEAMKLRQAVNLEEAVSDIGATAKALRAEIGASGKVAAVGYCFGGLLSYMAAARGMVDAAVPYYGGGIQNNLKEAEALNVPVQFHYGALDAHIKPEDVERVRQAVAGKRGVEVFVYDQADHGFNCWARGSYHQRSAALAHGRALTFLTNALS</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-10-07" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LHG5</accession>
    <name>Q1LHG5_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_3539</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10411.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF10411">
        <property type="protein sequence ID" value="ABF10411"/>
        <property type="gene ID" value="Rmet_3539"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3539"/>
    <dbReference type="HOGENOM" id="CLU_2603481_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF10411.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="79" mass="8915" checksum="189E2172CCCAF05A" modified="2006-05-30" version="1">MRRDIPQCLDQTRLDGIIQGLSAITKVAMPTVRFYPRRRRKSFVDNVPRLARTCASSGVWRRHGGVMRYGLETPAGITG</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LHS9</accession>
    <name>Q1LHS9_CUPMC</name>
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        <submittedName>
            <fullName evidence="3">Cysteine and O-acetyl-L-serine efflux system</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">eamA</name>
        <name evidence="3" type="ordered locus">Rmet_3425</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10297.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517859.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3425"/>
    <dbReference type="EnsemblBacteria" id="ABF10297">
        <property type="protein sequence ID" value="ABF10297"/>
        <property type="gene ID" value="Rmet_3425"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3425"/>
    <dbReference type="eggNOG" id="COG0697">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033863_20_1_4"/>
    <dbReference type="OMA" id="NTLTWPV"/>
    <dbReference type="OrthoDB" id="1515911at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000620">
        <property type="entry name" value="EamA_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00892">
        <property type="entry name" value="EamA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="33"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="61"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="87"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="116"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="141"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="171"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="205"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="241"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="266"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="domain" description="EamA" evidence="2">
        <location>
            <begin position="7"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="domain" description="EamA" evidence="2">
        <location>
            <begin position="144"/>
            <end position="283"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF10297.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="297" mass="31270" checksum="8C2E9982EE7D32DC" modified="2006-05-30" version="1">MQAKDRLLALAIIVVWGVNFVVIKVGLVGVPPMLLGALRFCLVAFPAVFFIPRPRIPLRMLVAYGATISLGQFVFLFYAMAVGMPAGLASLVLQSQVFFTVAIAGFWLGEPVRWHNIAGMAIAACGLALIGSGAAHGPGGMTLAGFLLTLCAALCWATGNIVSKKIGPVDLLGLVVWGALIPIVPFALLSLWFEGPARMTQALTNISGMGVFAVCYLAFCATLFGYTMWGRLLTRYAASKVAPLTLLVPVVGLVSAHLLLGEALALTQWAGALVVMAGLLLNVFGGRLWAGRMLAGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LHU9</accession>
    <name>Q1LHU9_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Transporter, LysE-family (Amino acid efflux)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Rmet_3405</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10277.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3405"/>
    <dbReference type="EnsemblBacteria" id="ABF10277">
        <property type="protein sequence ID" value="ABF10277"/>
        <property type="gene ID" value="Rmet_3405"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3405"/>
    <dbReference type="eggNOG" id="COG1280">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_079569_2_1_4"/>
    <dbReference type="OMA" id="WSGACLH"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006865">
        <property type="term" value="P:amino acid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001123">
        <property type="entry name" value="LeuE-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30086">
        <property type="entry name" value="PTHR30086"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01810">
        <property type="entry name" value="LysE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006324">
        <property type="entry name" value="LeuE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="6"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="43"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="74"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="115"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="149"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="184"/>
            <end position="206"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABF10277.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="212" mass="22449" checksum="5B8171254E8A3A64" modified="2006-05-30" version="1">MLPMDATTLFLYMIAVGAVVVTPGPTMLLALSNGATRGMRVAACGIAGAALADLILIGAVGCGLGAVLLASEQMFAVIKWIGAAYLLYLAWGLWRAPVQALTVSSGEASVNGWAAFLRALFVALSNPKTLLFTSAFVPQFIHPDKPVALQYTILAVVAAILDILMMMIYAFGGQHAMRTMSARAVRWVNRGCAGILAFLAVGLSLYRRSELR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="122" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LIV0</accession>
    <name>Q1LIV0_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 8">Shikimate dehydrogenase (NADP(+))</fullName>
            <shortName evidence="8">SDH</shortName>
            <ecNumber evidence="3 8">1.1.1.25</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8 12" type="primary">aroE</name>
        <name evidence="12" type="ordered locus">Rmet_3054</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Involved in the biosynthesis of the chorismate, which leads to the biosynthesis of aromatic amino acids. Catalyzes the reversible NADPH linked reduction of 3-dehydroshikimate (DHSA) to yield shikimate (SA).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 8">
            <text>NADP(+) + shikimate = 3-dehydroshikimate + H(+) + NADPH</text>
            <dbReference type="Rhea" id="RHEA:17737"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16630"/>
            <dbReference type="ChEBI" id="CHEBI:36208"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.1.1.25"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 8">Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 4/7.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the shikimate dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.25" evidence="3 8"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09926.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517556.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3054"/>
    <dbReference type="EnsemblBacteria" id="ABF09926">
        <property type="protein sequence ID" value="ABF09926"/>
        <property type="gene ID" value="Rmet_3054"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3054"/>
    <dbReference type="eggNOG" id="COG0169">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_044063_2_1_4"/>
    <dbReference type="OMA" id="FGNPIKH"/>
    <dbReference type="OrthoDB" id="1054867at2"/>
    <dbReference type="UniPathway" id="UPA00053">
        <property type="reaction ID" value="UER00087"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004764">
        <property type="term" value="F:shikimate 3-dehydrogenase (NADP+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009073">
        <property type="term" value="P:aromatic amino acid family biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008652">
        <property type="term" value="P:cellular amino acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009423">
        <property type="term" value="P:chorismate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019632">
        <property type="term" value="P:shikimate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00222">
        <property type="entry name" value="Shikimate_DH_AroE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046346">
        <property type="entry name" value="Aminiacid_DH-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041121">
        <property type="entry name" value="SDH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011342">
        <property type="entry name" value="Shikimate_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013708">
        <property type="entry name" value="Shikimate_DH-bd_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022893">
        <property type="entry name" value="Shikimate_DH_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006151">
        <property type="entry name" value="Shikm_DH/Glu-tRNA_Rdtase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21089">
        <property type="entry name" value="PTHR21089"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18317">
        <property type="entry name" value="SDH_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01488">
        <property type="entry name" value="Shikimate_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08501">
        <property type="entry name" value="Shikimate_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53223">
        <property type="entry name" value="SSF53223"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00507">
        <property type="entry name" value="aroE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="7 8" id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword evidence="5 8" id="KW-0521">NADP</keyword>
    <keyword evidence="6 8 12" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Shikimate_dh_N" evidence="10">
        <location>
            <begin position="23"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="domain" description="Shikimate_DH" evidence="9">
        <location>
            <begin position="134"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="domain" description="SDH_C" evidence="11">
        <location>
            <begin position="263"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="region of interest" description="Shikimate binding" evidence="8">
        <location>
            <begin position="31"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="8">
        <location>
            <position position="82"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="78"/>
        </location>
        <ligand>
            <name>shikimate</name>
            <dbReference type="ChEBI" id="CHEBI:36208"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="103"/>
        </location>
        <ligand>
            <name>shikimate</name>
            <dbReference type="ChEBI" id="CHEBI:36208"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="118"/>
        </location>
        <ligand>
            <name>shikimate</name>
            <dbReference type="ChEBI" id="CHEBI:36208"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="143"/>
            <end position="147"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="167"/>
            <end position="172"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="240"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="242"/>
        </location>
        <ligand>
            <name>shikimate</name>
            <dbReference type="ChEBI" id="CHEBI:36208"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="263"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="270"/>
        </location>
        <ligand>
            <name>shikimate</name>
            <dbReference type="ChEBI" id="CHEBI:36208"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001648"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004871"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF08501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF18317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ABF09926.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="302" mass="32214" checksum="080F001F2A988D48" modified="2006-05-30" version="1">MTADQNSQPNTAEPADSVDRYVVVGNPISHSRSPAIHAAFARQTGEAVQYDRLEAPLDAFADTMREFLAGGGHGFNVTVPFKLEAYDLADRLTPRAEAAGAVNTMWIEDGLIHGDNTDGIGLVRDIQDNLDVLLEDKRILVLGAGGAAMGAMLPLIECRPARIVVANRTASRASDMVEEFVEAADQYNVELWAGGLDALDALSEDERCDVVINASSSSLQGEVPPVPEHLLGEGVLAYDMMYGAQPTVFLAYAAKCGARTADGLGMLVEQAAEAFYNWRGVRPSTAPVLAELRAALQAEAAR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-02-05" modified="2022-08-03" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LJA1</accession>
    <name>OTC_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Ornithine carbamoyltransferase</fullName>
            <shortName evidence="2">OTCase</shortName>
            <ecNumber evidence="2">2.1.3.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">argF</name>
        <name type="ordered locus">Rmet_2902</name>
    </gene>
    <organism>
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Reversibly catalyzes the transfer of the carbamoyl group from carbamoyl phosphate (CP) to the N(epsilon) atom of ornithine (ORN) to produce L-citrulline.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>carbamoyl phosphate + L-ornithine = H(+) + L-citrulline + phosphate</text>
            <dbReference type="Rhea" id="RHEA:19513"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:46911"/>
            <dbReference type="ChEBI" id="CHEBI:57743"/>
            <dbReference type="ChEBI" id="CHEBI:58228"/>
            <dbReference type="EC" id="2.1.3.3"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Amino-acid biosynthesis; L-arginine biosynthesis; L-arginine from L-ornithine and carbamoyl phosphate: step 1/3.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the aspartate/ornithine carbamoyltransferase superfamily. OTCase family.</text>
    </comment>
    <dbReference type="EC" id="2.1.3.3" evidence="2"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09775.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517450.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q1LJA1"/>
    <dbReference type="SMR" id="Q1LJA1"/>
    <dbReference type="STRING" id="266264.Rmet_2902"/>
    <dbReference type="PRIDE" id="Q1LJA1"/>
    <dbReference type="EnsemblBacteria" id="ABF09775">
        <property type="protein sequence ID" value="ABF09775"/>
        <property type="gene ID" value="Rmet_2902"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2902"/>
    <dbReference type="eggNOG" id="COG0078">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_043846_3_2_4"/>
    <dbReference type="OMA" id="DGNNVCN"/>
    <dbReference type="OrthoDB" id="988597at2"/>
    <dbReference type="UniPathway" id="UPA00068">
        <property type="reaction ID" value="UER00112"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016597">
        <property type="term" value="F:amino acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004585">
        <property type="term" value="F:ornithine carbamoyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006526">
        <property type="term" value="P:arginine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1370">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01109">
        <property type="entry name" value="OTCase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006132">
        <property type="entry name" value="Asp/Orn_carbamoyltranf_P-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006130">
        <property type="entry name" value="Asp/Orn_carbamoylTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036901">
        <property type="entry name" value="Asp/Orn_carbamoylTrfase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006131">
        <property type="entry name" value="Asp_carbamoyltransf_Asp/Orn-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002292">
        <property type="entry name" value="Orn/put_carbamltrans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024904">
        <property type="entry name" value="OTCase_ArgI"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00185">
        <property type="entry name" value="OTCace"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02729">
        <property type="entry name" value="OTCace_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00100">
        <property type="entry name" value="AOTCASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00102">
        <property type="entry name" value="OTCASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53671">
        <property type="entry name" value="SSF53671"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00658">
        <property type="entry name" value="orni_carb_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00097">
        <property type="entry name" value="CARBAMOYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0055">Arginine biosynthesis</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_1000065114" description="Ornithine carbamoyltransferase">
        <location>
            <begin position="1"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="56"/>
            <end position="59"/>
        </location>
        <ligand>
            <name>carbamoyl phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58228"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="83"/>
        </location>
        <ligand>
            <name>carbamoyl phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58228"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="107"/>
        </location>
        <ligand>
            <name>carbamoyl phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58228"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="134"/>
            <end position="137"/>
        </location>
        <ligand>
            <name>carbamoyl phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58228"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="165"/>
        </location>
        <ligand>
            <name>L-ornithine</name>
            <dbReference type="ChEBI" id="CHEBI:46911"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="223"/>
        </location>
        <ligand>
            <name>L-ornithine</name>
            <dbReference type="ChEBI" id="CHEBI:46911"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="227"/>
            <end position="228"/>
        </location>
        <ligand>
            <name>L-ornithine</name>
            <dbReference type="ChEBI" id="CHEBI:46911"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="263"/>
            <end position="264"/>
        </location>
        <ligand>
            <name>carbamoyl phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58228"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="291"/>
        </location>
        <ligand>
            <name>carbamoyl phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58228"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01109"/>
        </source>
    </evidence>
    <sequence length="307" mass="35015" checksum="F8BD69B970FA33D6" modified="2006-05-30" version="1">MSPTPIKHYLQFSDLSADEYEYLLDRARILKAKFKNYETWHPLHDRTLAMIFEKNSTRTRLSFEAGIHQLGGHAVFLNTRDSQLGRGEPIEDAAQVISRMVDIIMIRTFGQEIIDRFAKHSRVPVINGLTNEYHPCQVLADVFTYIEQRGTIAGKTVAWIGDANNMAYTWIQAAERLDFTFHFSAPPGYQLDPAMVPDWAASRVKVFDNPLDACQGAHLVTTDVWTSMGFEAENDARKRAFKDWMVTTAMMDRAADDALFMHCLPAHRGEEVEAAVIDGPRSVVWEEAENRLHVQKALMEYLLCGRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-10-07" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LJC1</accession>
    <name>Q1LJC1_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Retrotransposon protein, putative, Ty1-copia subclass from Oryza sativa (Japonica cultivar-group)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_2882</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09755.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF09755">
        <property type="protein sequence ID" value="ABF09755"/>
        <property type="gene ID" value="Rmet_2882"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2882"/>
    <dbReference type="HOGENOM" id="CLU_2059452_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF09755.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="119" mass="13004" checksum="B47577433F3BA571" modified="2006-05-30" version="1">MRFACRSHTALRQPYGLTTFPAHYTTGLGPASSPVVRMATCSELSAEQPTTYLLVSAYQQLWHCNAYGVYQQFTYVAHTSQPGSSTPLLLGVSASLPLGSYAPLRGLRCQESFTPHSYP</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="112" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LJE8</accession>
    <name>Q1LJE8_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 5 7">Histidine ammonia-lyase</fullName>
            <shortName evidence="5">Histidase</shortName>
            <ecNumber evidence="3 5 7">4.3.1.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5 9" type="primary">hutH</name>
        <name evidence="9" type="ordered locus">Rmet_2855</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 5 7">
            <text>L-histidine = NH4(+) + trans-urocanate</text>
            <dbReference type="Rhea" id="RHEA:21232"/>
            <dbReference type="ChEBI" id="CHEBI:17771"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:57595"/>
            <dbReference type="EC" id="4.3.1.3"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 5 7">Amino-acid degradation; L-histidine degradation into L-glutamate; N-formimidoyl-L-glutamate from L-histidine: step 1/3.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5 8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="PTM">
        <text evidence="5">Contains an active site 4-methylidene-imidazol-5-one (MIO), which is formed autocatalytically by cyclization and dehydration of residues Ala-Ser-Gly.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5 6">Belongs to the PAL/histidase family.</text>
    </comment>
    <dbReference type="EC" id="4.3.1.3" evidence="3 5 7"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09728.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517413.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2855"/>
    <dbReference type="EnsemblBacteria" id="ABF09728">
        <property type="protein sequence ID" value="ABF09728"/>
        <property type="gene ID" value="Rmet_2855"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2855"/>
    <dbReference type="eggNOG" id="COG2986">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014801_4_0_4"/>
    <dbReference type="OMA" id="CAPQVAG"/>
    <dbReference type="OrthoDB" id="715502at2"/>
    <dbReference type="UniPathway" id="UPA00379">
        <property type="reaction ID" value="UER00549"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004397">
        <property type="term" value="F:histidine ammonia-lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019556">
        <property type="term" value="P:histidine catabolic process to glutamate and formamide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019557">
        <property type="term" value="P:histidine catabolic process to glutamate and formate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00332">
        <property type="entry name" value="PAL-HAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.275.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00229">
        <property type="entry name" value="His_ammonia_lyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001106">
        <property type="entry name" value="Aromatic_Lyase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024083">
        <property type="entry name" value="Fumarase/histidase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005921">
        <property type="entry name" value="HutH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008948">
        <property type="entry name" value="L-Aspartase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022313">
        <property type="entry name" value="Phe/His_NH3-lyase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10362">
        <property type="entry name" value="PTHR10362"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00221">
        <property type="entry name" value="Lyase_aromatic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48557">
        <property type="entry name" value="SSF48557"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01225">
        <property type="entry name" value="hutH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00488">
        <property type="entry name" value="PAL_HISTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 5 7" id="KW-0369">Histidine metabolism</keyword>
    <keyword evidence="5 6 9" id="KW-0456">Lyase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="modified residue" description="2,3-didehydroalanine (Ser)" evidence="5">
        <location>
            <position position="151"/>
        </location>
    </feature>
    <feature type="cross-link" description="5-imidazolinone (Ala-Gly)" evidence="5">
        <location>
            <begin position="150"/>
            <end position="152"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU004480"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ABF09728.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="522" mass="54122" checksum="742C5373861F714D" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="70" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LJM8</accession>
    <name>Q1LJM8_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">PepSY-associated TM helix</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_2775</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09648.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517346.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2775"/>
    <dbReference type="EnsemblBacteria" id="ABF09648">
        <property type="protein sequence ID" value="ABF09648"/>
        <property type="gene ID" value="Rmet_2775"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2775"/>
    <dbReference type="eggNOG" id="COG3182">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031962_4_2_4"/>
    <dbReference type="OMA" id="HRGRFFG"/>
    <dbReference type="OrthoDB" id="1656356at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025711">
        <property type="entry name" value="PepSY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005625">
        <property type="entry name" value="PepSY-ass_TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34219">
        <property type="entry name" value="PTHR34219"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03413">
        <property type="entry name" value="PepSY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03929">
        <property type="entry name" value="PepSY_TM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="15"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="158"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="208"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="354"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="domain" description="PepSY" evidence="2">
        <location>
            <begin position="260"/>
            <end position="322"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF03413"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF09648.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="393" mass="43658" checksum="68D76DA1AAD900E9" modified="2006-05-30" version="1">MLSGRLRTFFVKLHLYIGLVFGVLFILMGLTGTAISWRDEIDKWLNPDLLQAVSATRIPVGAATVESVTAKLMADPHFGKPNLLMLPTDEHDVFVAYYPIKGPEAGPGRFRQVMIDPITLAVKGERLWGVPGLSRQALMPTVFHLHRYLLSGDTGKTITGVTGMMLFISVLLGVVLWWPKFKWRSLKQSVRITHGGSWSRFIYTSHRAGGIFVAPILATIAFSGWYLNLPKWVTPIIASVATLSPQIKPVSDPAPEGTHAITPAQAMTAAQKQFPDAAVTRISLPRKPGDAYEVRLRQEGEVRADTGATRLWLDAYTGRPLAVRDPRNAASGDTIINWLFPLHTGEAFGSWGRAFITVFGLVPLAFAVTGVLIWWKRRSGHRRHLERERLRRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="102" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LK02</accession>
    <name>Q1LK02_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Transcriptional regulator, AsnC/Lrp-family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Rmet_2647</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09524.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517222.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2647"/>
    <dbReference type="EnsemblBacteria" id="ABF09524">
        <property type="protein sequence ID" value="ABF09524"/>
        <property type="gene ID" value="Rmet_2647"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2647"/>
    <dbReference type="eggNOG" id="COG1522">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_091233_0_3_4"/>
    <dbReference type="OMA" id="CSRRLDI"/>
    <dbReference type="OrthoDB" id="1534467at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="CDD" id="cd00090">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011991">
        <property type="entry name" value="ArsR-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000485">
        <property type="entry name" value="AsnC-type_HTH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011008">
        <property type="entry name" value="Dimeric_a/b-barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019888">
        <property type="entry name" value="Tscrpt_reg_AsnC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019887">
        <property type="entry name" value="Tscrpt_reg_AsnC/Lrp_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019885">
        <property type="entry name" value="Tscrpt_reg_HTH_AsnC-type_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01037">
        <property type="entry name" value="AsnC_trans_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00033">
        <property type="entry name" value="HTHASNC"/>
    </dbReference>
    <dbReference type="SMART" id="SM00344">
        <property type="entry name" value="HTH_ASNC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54909">
        <property type="entry name" value="SSF54909"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00519">
        <property type="entry name" value="HTH_ASNC_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50956">
        <property type="entry name" value="HTH_ASNC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH asnC-type" evidence="4">
        <location>
            <begin position="3"/>
            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50956"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABF09524.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="159" mass="17893" checksum="CBF474BE38103238" modified="2006-05-30" version="1">MNLDSIDLRILTCLQENGRISNQDLADRVALSPSACLRRVRLLEESGVIGGYHAWFDAEKLGLEIEAIVQVSMRHDVEGWHDTFNAVVQTWPEVLTAYVITGDSNYILRVQARNLKHYSDFIVNRLYRTPGVMDIRSNIVLQRIKTDGSPLAIVKEAGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LL95</accession>
    <name>Q1LL95_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">2-aminobenzoyl-CoA monooxygenase/reductase</fullName>
            <ecNumber evidence="2">1.14.13.40</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">abmA</name>
        <name evidence="2" type="ordered locus">Rmet_2202</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EC" id="1.14.13.40" evidence="2"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09081.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516909.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2202"/>
    <dbReference type="EnsemblBacteria" id="ABF09081">
        <property type="protein sequence ID" value="ABF09081"/>
        <property type="gene ID" value="Rmet_2202"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2202"/>
    <dbReference type="eggNOG" id="COG0654">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG1902">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012153_10_0_4"/>
    <dbReference type="OMA" id="PMFTPFR"/>
    <dbReference type="OrthoDB" id="1033671at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018673">
        <property type="term" value="F:anthraniloyl-CoA monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003959">
        <property type="term" value="F:NADPH dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02932">
        <property type="entry name" value="OYE_YqiM_FMN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001155">
        <property type="entry name" value="OxRdtase_FMN_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044152">
        <property type="entry name" value="YqjM-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43303">
        <property type="entry name" value="PTHR43303"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00724">
        <property type="entry name" value="Oxidored_FMN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="SSF51905"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Oxidored_FMN" evidence="1">
        <location>
            <begin position="421"/>
            <end position="750"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00724"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF09081.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="791" mass="87373" checksum="D66B682F22618A46" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LLD6</accession>
    <name>Q1LLD6_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Piwi domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_2161</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09040.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516870.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF09040">
        <property type="protein sequence ID" value="ABF09040"/>
        <property type="gene ID" value="Rmet_2161"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2161"/>
    <dbReference type="eggNOG" id="COG1431">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031104_1_0_4"/>
    <dbReference type="OMA" id="CCAAQMF"/>
    <dbReference type="OrthoDB" id="404766at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003165">
        <property type="entry name" value="Piwi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="SMART" id="SM00950">
        <property type="entry name" value="Piwi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="SSF53098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Piwi" evidence="1">
        <location>
            <begin position="197"/>
            <end position="467"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00950"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF09040.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="483" mass="54636" checksum="5739AED9537BBFD0" modified="2006-05-30" version="1">MMKLDFLKEPELEFGGHHRHVDMRQGIALFGPLDSIEGQPRRIRLGVVGTQQTVDGILQWLERSKAGIKAKASRQPHLFPDFPGFGADSPFRADFNIDASSVRTLSSRELSRITAQSELMAVADGQSMLADEVQAISESGRPDVVIVGVPQELVALEDNDANEATAEFNPKQDALEYGSTFDLRNMLKAKVMRMNQPIQLVLPQTYDETAKGKKSKKGEKRALQDEATRAWNFHTALYYKANYRPWRIVRDPSEFQACFVGISFYRSLDQATLVTSMAQVYDERGEGVIVRGKAVELLKDDRQPHLSRNDAYLLLTTALKEYRREHRHLPARVVVHKSSKYSKDELEGFNGAVDDQGLETADFMVVTKSFTRLFREGTYPPLRGTMLSLDEANHLLYTRGSVPYYATYPGMYVPRPLKFQIAQADTSPRKLAEEILALTKLNWNNTQFDGAEPITMRVAQQVGKVLKYVPEGDVVQPRYSFYM</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-12-02" version="69" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LLL8</accession>
    <name>Q1LLL8_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Cytochrome c55X (Precursor)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">nirC</name>
        <name evidence="6" type="ordered locus">Rmet_2079</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08958.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2079"/>
    <dbReference type="EnsemblBacteria" id="ABF08958">
        <property type="protein sequence ID" value="ABF08958"/>
        <property type="gene ID" value="Rmet_2079"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2079"/>
    <dbReference type="eggNOG" id="COG2010">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_159748_0_0_4"/>
    <dbReference type="OMA" id="DCGACHG"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.760.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009056">
        <property type="entry name" value="Cyt_c-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036909">
        <property type="entry name" value="Cyt_c-like_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13442">
        <property type="entry name" value="Cytochrome_CBB3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46626">
        <property type="entry name" value="SSF46626"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51007">
        <property type="entry name" value="CYTC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 4" id="KW-0349">Heme</keyword>
    <keyword evidence="3 4" id="KW-0408">Iron</keyword>
    <keyword evidence="2 4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cytochrome c" evidence="5">
        <location>
            <begin position="41"/>
            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABF08958.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="129" mass="13595" checksum="D0377EB99ABFAE91" modified="2006-05-30" version="1">MMSAFAPLAGEPMPTLRWICIPILSCIAATAIAAEPPAAAPTPARQAELFRLLRDDCGACHGMTLQGGLGSPLTAAALADKPRDGLVATVLQGRPGTAMPPWRPFMTQDEARWLIDRLQSGQVPAVPKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="99" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LLY1</accession>
    <name>Q1LLY1_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7 16">Homoserine dehydrogenase</fullName>
            <ecNumber evidence="6 16">1.1.1.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="primary">thrA</name>
        <name evidence="19" type="ordered locus">Rmet_1966</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>L-homoserine + NAD(+) = H(+) + L-aspartate 4-semialdehyde + NADH</text>
            <dbReference type="Rhea" id="RHEA:15757"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57476"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:537519"/>
            <dbReference type="EC" id="1.1.1.3"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 16">
            <text>L-homoserine + NADP(+) = H(+) + L-aspartate 4-semialdehyde + NADPH</text>
            <dbReference type="Rhea" id="RHEA:15761"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57476"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:537519"/>
            <dbReference type="EC" id="1.1.1.3"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="4 16">Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-homoserine from L-aspartate: step 3/3.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3 16">Amino-acid biosynthesis; L-threonine biosynthesis; L-threonine from L-aspartate: step 3/5.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5 17">Belongs to the homoserine dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.3" evidence="6 16"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08845.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516690.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1966"/>
    <dbReference type="EnsemblBacteria" id="ABF08845">
        <property type="protein sequence ID" value="ABF08845"/>
        <property type="gene ID" value="Rmet_1966"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1966"/>
    <dbReference type="eggNOG" id="COG0460">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009116_1_0_4"/>
    <dbReference type="OMA" id="LMFYGPG"/>
    <dbReference type="OrthoDB" id="1464088at2"/>
    <dbReference type="UniPathway" id="UPA00050">
        <property type="reaction ID" value="UER00063"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00051">
        <property type="reaction ID" value="UER00465"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004412">
        <property type="term" value="F:homoserine dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009097">
        <property type="term" value="P:isoleucine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009086">
        <property type="term" value="P:methionine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009088">
        <property type="term" value="P:threonine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045865">
        <property type="entry name" value="ACT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002912">
        <property type="entry name" value="ACT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005106">
        <property type="entry name" value="Asp/hSer_DH_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016204">
        <property type="entry name" value="HDH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001342">
        <property type="entry name" value="HDH_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019811">
        <property type="entry name" value="HDH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01842">
        <property type="entry name" value="ACT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00742">
        <property type="entry name" value="Homoserine_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03447">
        <property type="entry name" value="NAD_binding_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000098">
        <property type="entry name" value="Homoser_dehydrog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55021">
        <property type="entry name" value="SSF55021"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51671">
        <property type="entry name" value="ACT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01042">
        <property type="entry name" value="HOMOSER_DHGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 16" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="16" id="KW-0100">Branched-chain amino acid biosynthesis</keyword>
    <keyword evidence="9 16" id="KW-0412">Isoleucine biosynthesis</keyword>
    <keyword evidence="13 16" id="KW-0486">Methionine biosynthesis</keyword>
    <keyword evidence="11 15 16" id="KW-0521">NADP</keyword>
    <keyword evidence="12 16 19" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10 16" id="KW-0791">Threonine biosynthesis</keyword>
    <feature type="domain" description="ACT" evidence="18">
        <location>
            <begin position="355"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="14">
        <location>
            <position position="205"/>
        </location>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <begin position="9"/>
            <end position="16"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="105"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="190"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005062"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00006753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00013213"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00013376"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022624"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR000098-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR000098-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU000579"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU004171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="ABF08845.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="436" mass="47153" checksum="6D0650672BA697C9" modified="2006-05-30" version="1">MNPIKVGLLGIGTVGSGTFNVLKRNQEEIRRRAGRGIEIAVVADLNTERARELTGGTVDVVSDANDVVTRPDIDIVIELIGGYGIARELVLKAIENGKHVVTANKALLAVHGNEIFEAARKKGVIVAFEAAVAGGIPIIKALREGLTANRIQWIAGIINGTTNFILSEMRDKGLDFDTVLKEAQQLGYAEADPTFDIEGVDAAHKVTLMSAIAFGMPVQFDRAHVEGITKLSAIDIKYAEELGYRIKLLGITRRREEGVELRVHPTLVPASRLIANVEGAMNAVLVQGDAVGATLYYGKGAGAEPTASAVIADLVDVTRLHTADPNHRVPHLAFQPDELSNVPVLPIDEVTSSYYLRMRVSDETGVLAEITRILAEAGISIDAMLQKESREGEPQTDIIILTHLTREKHVNAAIRSIEALQTVLSPVTRLRMEELN</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="69" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LM44</accession>
    <name>Q1LM44_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Fatty acid hydroxylase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_1903</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08782.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516636.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1903"/>
    <dbReference type="DNASU" id="4038707"/>
    <dbReference type="EnsemblBacteria" id="ABF08782">
        <property type="protein sequence ID" value="ABF08782"/>
        <property type="gene ID" value="Rmet_1903"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1903"/>
    <dbReference type="eggNOG" id="COG3000">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033631_3_1_4"/>
    <dbReference type="OMA" id="HQIHHSP"/>
    <dbReference type="OrthoDB" id="2048011at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008610">
        <property type="term" value="P:lipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006694">
        <property type="entry name" value="Fatty_acid_hydroxylase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04116">
        <property type="entry name" value="FA_hydroxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="33"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="68"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="134"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="domain" description="Fatty acid hydroxylase" evidence="2">
        <location>
            <begin position="75"/>
            <end position="206"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF04116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF08782.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="245" mass="28911" checksum="E9C2D105EB0A1F3A" modified="2006-05-30" version="1">MISVILIVFACCFVLERIAPSWPLPRVRTWPVRVLLVNAVQLGVVLLAGMSWERWLASWSLFRLSDHVSPAAGGLLAYFIATFVFYWWHRWRHESDLLWRLFHQIHHSPQRLEVITSFYKHPAEMVVNSIIGSLLVYTLLGLSLEAGGVYTLCTALGEFFYHTNVRSPRWVGFVFQRPEMHRIHHRHGHHRNNYGDITWWDMLFGTYENPAEWRDSCGFDNNREQRLTEMLAWRDVHADKRPDSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LM52</accession>
    <name>Q1LM52_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Thiamine pyrophosphate-requiring enzyme (Multidomain protein)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Rmet_1895</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TPP enzyme family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08774.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516626.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1895"/>
    <dbReference type="EnsemblBacteria" id="ABF08774">
        <property type="protein sequence ID" value="ABF08774"/>
        <property type="gene ID" value="Rmet_1895"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1895"/>
    <dbReference type="eggNOG" id="COG0028">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013748_8_0_4"/>
    <dbReference type="OMA" id="CGVEVCF"/>
    <dbReference type="OrthoDB" id="391134at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030976">
        <property type="term" value="F:thiamine pyrophosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044281">
        <property type="term" value="P:small molecule metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012001">
        <property type="entry name" value="Thiamin_PyroP_enz_TPP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000399">
        <property type="entry name" value="TPP-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045229">
        <property type="entry name" value="TPP_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011766">
        <property type="entry name" value="TPP_enzyme-bd_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18968">
        <property type="entry name" value="PTHR18968"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02775">
        <property type="entry name" value="TPP_enzyme_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02776">
        <property type="entry name" value="TPP_enzyme_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="SSF52518"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00187">
        <property type="entry name" value="TPP_ENZYMES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TPP_enzyme_N" evidence="3">
        <location>
            <begin position="1"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="domain" description="TPP_enzyme_C" evidence="2">
        <location>
            <begin position="365"/>
            <end position="512"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007812"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF02776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF08774.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="514" mass="53457" checksum="446F4EC0D95AB9B3" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LME3</accession>
    <name>Q1LME3_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Oxidoreductase, 2OG-Fe(II) oxygenase family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Rmet_1804</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the iron/ascorbate-dependent oxidoreductase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08683.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516533.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1804"/>
    <dbReference type="EnsemblBacteria" id="ABF08683">
        <property type="protein sequence ID" value="ABF08683"/>
        <property type="gene ID" value="Rmet_1804"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1804"/>
    <dbReference type="eggNOG" id="COG3145">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1080430_0_0_4"/>
    <dbReference type="OMA" id="ELHTRNP"/>
    <dbReference type="OrthoDB" id="1892914at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051213">
        <property type="term" value="F:dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005123">
        <property type="entry name" value="Oxoglu/Fe-dep_dioxygenase"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51471">
        <property type="entry name" value="FE2OG_OXY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="2" id="KW-0408">Iron</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fe2OG dioxygenase" evidence="4">
        <location>
            <begin position="129"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU003682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABF08683.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="267" mass="29765" checksum="D24EAC38D203D392" modified="2006-05-30" version="1">MSEHASSDIALAPPPARPATSQPDAAVAHAIEQLDTPALRADFERQGAFLYLDKFLAPDECAQLVALTRAMQPGLNRNYLPGHKQGGSVSRHTIDEQAPFIADLYRSKALIGWLEKITGDKLQVCPDTDPHAYALYYYTRQGDHIGWHYDTSYYDGRRYTLLIGVLDDSSCLLEYELHTRTPEIPDEPGAVQIPPGGIVLFDGDKLRHRVTPAGPNETRVSLTFEYVTDPGMRPWLRFISNMKDAVAYFGFRQVFRRLLGGANSSGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LME5</accession>
    <name>Q1LME5_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Sulfatase</fullName>
            <ecNumber evidence="3">3.1.6.6</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">yidJ</name>
        <name evidence="3" type="ordered locus">Rmet_1802</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="PTM">
        <text evidence="1">The conversion to 3-oxoalanine (also known as C-formylglycine, FGly), of a serine or cysteine residue in prokaryotes and of a cysteine residue in eukaryotes, is critical for catalytic activity.</text>
    </comment>
    <dbReference type="EC" id="3.1.6.6" evidence="3"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08681.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516531.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1802"/>
    <dbReference type="EnsemblBacteria" id="ABF08681">
        <property type="protein sequence ID" value="ABF08681"/>
        <property type="gene ID" value="Rmet_1802"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1802"/>
    <dbReference type="eggNOG" id="COG3119">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006332_9_2_4"/>
    <dbReference type="OMA" id="DHGELHG"/>
    <dbReference type="OrthoDB" id="1067869at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047753">
        <property type="term" value="F:choline-sulfatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.720.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017850">
        <property type="entry name" value="Alkaline_phosphatase_core_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000917">
        <property type="entry name" value="Sulfatase_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00884">
        <property type="entry name" value="Sulfatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53649">
        <property type="entry name" value="SSF53649"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Sulfatase" evidence="2">
        <location>
            <begin position="4"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="modified residue" description="3-oxoalanine (Cys)" evidence="1">
        <location>
            <position position="52"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRSR" id="PIRSR600917-51"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF08681.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="518" mass="58528" checksum="AFC5BEF5AA2E887E" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LMR9</accession>
    <name>Q1LMR9_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Universal stress protein, UspA family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">uspA4</name>
        <name evidence="3" type="ordered locus">Rmet_1676</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal stress protein A family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08557.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516405.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1676"/>
    <dbReference type="EnsemblBacteria" id="ABF08557">
        <property type="protein sequence ID" value="ABF08557"/>
        <property type="gene ID" value="Rmet_1676"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1676"/>
    <dbReference type="eggNOG" id="COG0589">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_049301_11_0_4"/>
    <dbReference type="OMA" id="DCIDRIA"/>
    <dbReference type="OrthoDB" id="2040681at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006015">
        <property type="entry name" value="Universal_stress_UspA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006016">
        <property type="entry name" value="UspA"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00582">
        <property type="entry name" value="Usp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01438">
        <property type="entry name" value="UNVRSLSTRESS"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Usp" evidence="2">
        <location>
            <begin position="1"/>
            <end position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008791"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF08557.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="143" mass="15216" checksum="A5263D6595259A17" modified="2006-05-30" version="1">MFKHILLPVDGSDQSRKAVSAAIEFARSTRARLTPYICKEEPRLSISMSAAVDTGSVESRLESAAREELSRIEAAAALAGVPCIGKTSSASAPHLGIIEKANELQCDVIFMASHGRKGLGKLLLGSVTQDVLSHSTIPVLVFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LMU4</accession>
    <name>Q1LMU4_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Putative ferritin/ribonucleotide reductase-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_1649</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08532.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516377.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1649"/>
    <dbReference type="EnsemblBacteria" id="ABF08532">
        <property type="protein sequence ID" value="ABF08532"/>
        <property type="gene ID" value="Rmet_1649"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1649"/>
    <dbReference type="eggNOG" id="COG1633">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_120367_0_0_4"/>
    <dbReference type="OMA" id="AMHWAIL"/>
    <dbReference type="OrthoDB" id="1485364at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1260.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012347">
        <property type="entry name" value="Ferritin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009078">
        <property type="entry name" value="Ferritin-like_SF"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47240">
        <property type="entry name" value="SSF47240"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF08532.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="206" mass="21595" checksum="3302486DAAEF7608" modified="2006-05-30" version="1">MKTKPDLSLPFAGSACDADLAPDLVRRGWLKAPGAFALGSLAVMSLGEAMPAWGQTHSTSTKDDINILNTALGLEYQAIAAYQVGAESGLLQKPVLDTAVKFQTHHKAHADVLANTVMKLGGTPVKTRKASEYNFPAADLKTQADVLRFAAGLEKGATAAYLGVLPSFHNRELAKSAGSILGDEAMHWAILLSVLGEDPVPGAFVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="98" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LMW3</accession>
    <name>Q1LMW3_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Potassium-efflux system protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Rmet_1630</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the monovalent cation:proton antiporter 2 (CPA2) transporter (TC 2.A.37) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08513.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516358.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1630"/>
    <dbReference type="EnsemblBacteria" id="ABF08513">
        <property type="protein sequence ID" value="ABF08513"/>
        <property type="gene ID" value="Rmet_1630"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1630"/>
    <dbReference type="eggNOG" id="COG0475">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005126_1_0_4"/>
    <dbReference type="OMA" id="MMMLYMG"/>
    <dbReference type="OrthoDB" id="941006at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005887">
        <property type="term" value="C:integral component of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019899">
        <property type="term" value="F:enzyme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015503">
        <property type="term" value="F:glutathione-regulated potassium exporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015299">
        <property type="term" value="F:solute:proton antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015643">
        <property type="term" value="F:toxic substance binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051595">
        <property type="term" value="P:response to methylglyoxal"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1530.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006153">
        <property type="entry name" value="Cation/H_exchanger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004771">
        <property type="entry name" value="K/H_exchanger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023941">
        <property type="entry name" value="K_H_efflux_KefC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038770">
        <property type="entry name" value="Na+/solute_symporter_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46157:SF3">
        <property type="entry name" value="PTHR46157:SF3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00999">
        <property type="entry name" value="Na_H_Exchanger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00932">
        <property type="entry name" value="2a37"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="51"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="84"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="112"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="144"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="177"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="216"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="292"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="324"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="356"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="domain" description="Na_H_Exchanger" evidence="6">
        <location>
            <begin position="8"/>
            <end position="372"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABF08513.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="404" mass="42774" checksum="FFFCBCC3EA8A97AA" modified="2006-05-30" version="1">MELLLQAVLFLASALVVVPLSIRLGFGSVLGYLVAGMLIGPSALGFVTDVNAVLHIAELGIMLMMFIIGIEMDVRKLWNLRRSIFGHGGAQVLLCAVLLALAFTAFGVNWRVGVAAGFALSLSSTAMVIAILEQRGLMQAPIGRASFGILLFQDMAAIPMIALLPLLSPAPATTNTIAGWIVALEALGMLAAVVFGGTILLRYLLPIIRRSGTRDMVTVFALLWVIGIALLMQSVHLSMSLGAFVAGVLLAGSDCREEIEADISPIKGVLLGLFFMAVGMSIKFDVLASKPFLVAVLLLVLLVAKISGLLFLARQIQLPRGDRVYFAVLLSQGGEFAFVVMAVAEAARLLNGEQASIVTAVVALSMAATPLLLMALRTKEVARDVERPLPAANRHADAPDADLH</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LN52</accession>
    <name>Q1LN52_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Hydrogenase maturation factor hoxX</fullName>
            <ecNumber evidence="4">2.1.2.9</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">hoxX</name>
        <name evidence="4" type="ordered locus">Rmet_1541</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.1.2.9" evidence="4"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08424.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516281.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1541"/>
    <dbReference type="EnsemblBacteria" id="ABF08424">
        <property type="protein sequence ID" value="ABF08424"/>
        <property type="gene ID" value="Rmet_1541"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1541"/>
    <dbReference type="eggNOG" id="COG0223">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG1024">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008537_1_0_4"/>
    <dbReference type="OMA" id="GFDPSYH"/>
    <dbReference type="OrthoDB" id="178084at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004479">
        <property type="term" value="F:methionyl-tRNA formyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029045">
        <property type="entry name" value="ClpP/crotonase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001753">
        <property type="entry name" value="Enoyl-CoA_hydra/iso"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005793">
        <property type="entry name" value="Formyl_trans_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002376">
        <property type="entry name" value="Formyl_transf_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036477">
        <property type="entry name" value="Formyl_transf_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011034">
        <property type="entry name" value="Formyl_transferase-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009188">
        <property type="entry name" value="NiFe-hyd_mat_HypX/HoxX"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00378">
        <property type="entry name" value="ECH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02911">
        <property type="entry name" value="Formyl_trans_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00551">
        <property type="entry name" value="Formyl_trans_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006787">
        <property type="entry name" value="Hydrgn_mat_HoxX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50486">
        <property type="entry name" value="SSF50486"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52096">
        <property type="entry name" value="SSF52096"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53328">
        <property type="entry name" value="SSF53328"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0371">Homeobox</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Formyl_trans_N" evidence="2">
        <location>
            <begin position="39"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="domain" description="Formyl_trans_C" evidence="3">
        <location>
            <begin position="177"/>
            <end position="261"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF02911"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF08424.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="579" mass="64528" checksum="FD2FB3E28F076137" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="106" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LN74</accession>
    <name>Q1LN74_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 12">Pyruvate kinase</fullName>
            <ecNumber evidence="4 12">2.7.1.40</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">pykA2</name>
        <name evidence="15" type="ordered locus">Rmet_1519</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>ATP + pyruvate = ADP + H(+) + phosphoenolpyruvate</text>
            <dbReference type="Rhea" id="RHEA:18157"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.40"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2 12">Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 5/5.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 12">Belongs to the pyruvate kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.40" evidence="4 12"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08402.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516262.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1519"/>
    <dbReference type="EnsemblBacteria" id="ABF08402">
        <property type="protein sequence ID" value="ABF08402"/>
        <property type="gene ID" value="Rmet_1519"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1519"/>
    <dbReference type="eggNOG" id="COG0469">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015439_0_2_4"/>
    <dbReference type="OMA" id="PGCHCAV"/>
    <dbReference type="OrthoDB" id="1098983at2"/>
    <dbReference type="UniPathway" id="UPA00109">
        <property type="reaction ID" value="UER00188"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030955">
        <property type="term" value="F:potassium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004743">
        <property type="term" value="F:pyruvate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.33.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1380.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001697">
        <property type="entry name" value="Pyr_Knase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015813">
        <property type="entry name" value="Pyrv/PenolPyrv_Kinase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040442">
        <property type="entry name" value="Pyrv_Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011037">
        <property type="entry name" value="Pyrv_Knase-like_insert_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018209">
        <property type="entry name" value="Pyrv_Knase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015793">
        <property type="entry name" value="Pyrv_Knase_brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015795">
        <property type="entry name" value="Pyrv_Knase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036918">
        <property type="entry name" value="Pyrv_Knase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015806">
        <property type="entry name" value="Pyrv_Knase_insert_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11817">
        <property type="entry name" value="PTHR11817"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00224">
        <property type="entry name" value="PK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02887">
        <property type="entry name" value="PK_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01050">
        <property type="entry name" value="PYRUVTKNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50800">
        <property type="entry name" value="SSF50800"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51621">
        <property type="entry name" value="SSF51621"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52935">
        <property type="entry name" value="SSF52935"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01064">
        <property type="entry name" value="pyruv_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00110">
        <property type="entry name" value="PYRUVATE_KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10 12" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="7 12 15" id="KW-0418">Kinase</keyword>
    <keyword evidence="9 12" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11 15" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12 15" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PK" evidence="13">
        <location>
            <begin position="5"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="domain" description="PK_C" evidence="14">
        <location>
            <begin position="357"/>
            <end position="469"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004997"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF02887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="ABF08402.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="472" mass="50108" checksum="B30C911C724CD6C6" modified="2006-05-30" version="1">MRRNRNAKIVATLGPSSSDKDTIRRLFAAGVDVFRLNFSHGDEAAHRASFAILRELEREAGRPIGILADLQGPKLRVGTFAAGSVMLDESQPFRLELDTAPGDARRVGLPHPEIFAALAPGAELLLDDGKLRLIVERCGADFADTRVVAGGKLSDRKGVNVPGVLLPISALTAKDRRDLALALDLGADWIALSFVQRPEDVVEARKLIGERAAIVAKLEKPSAVEQLEAIAECADAVMVARGDLGVELPAERVPGIQKRILRTCRRLGRPVIVATQMLESMVTSPVPTRAEASDVATAIYDGADAVMLSAESAAGRYPVEAVDMMSRLITEVEGDPQYLQLMNASRPSAPAGAGAADAVCCAVRHAVALMHASAIVCYTSSGHTSLRVARERPEAPVLSLTPHLRVARRMALAWGIHSVHIEDVADVDQMTAKACAVACQEGFAQGGQTLVVIAGVPFGTPGTTNLMRIATV</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="62" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LNA4</accession>
    <name>Q1LNA4_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Relaxase type IV secretory pathway VirD2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">virD2</name>
        <name evidence="2" type="ordered locus">Rmet_1489</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08372.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1489"/>
    <dbReference type="EnsemblBacteria" id="ABF08372">
        <property type="protein sequence ID" value="ABF08372"/>
        <property type="gene ID" value="Rmet_1489"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1489"/>
    <dbReference type="eggNOG" id="COG3843">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027492_1_0_4"/>
    <dbReference type="OMA" id="IDHWNTE"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021795">
        <property type="entry name" value="DUF3363"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11843">
        <property type="entry name" value="DUF3363"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="30"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="42"/>
            <end position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF08372.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="700" mass="76908" checksum="C46B72F0AB9AFD3E" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="97" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LNF3</accession>
    <name>Q1LNF3_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Phosphatidate cytidylyltransferase</fullName>
            <ecNumber evidence="7">2.7.7.41</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">cdsA</name>
        <name evidence="9" type="ordered locus">Rmet_1440</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>a 1,2-diacyl-sn-glycero-3-phosphate + CTP + H(+) = a CDP-1,2-diacyl-sn-glycerol + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:16229"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
            <dbReference type="ChEBI" id="CHEBI:58332"/>
            <dbReference type="ChEBI" id="CHEBI:58608"/>
            <dbReference type="EC" id="2.7.7.41"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="7">Phospholipid metabolism; CDP-diacylglycerol biosynthesis; CDP-diacylglycerol from sn-glycerol 3-phosphate: step 3/3.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the CDS family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.41" evidence="7"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08323.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516190.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1440"/>
    <dbReference type="EnsemblBacteria" id="ABF08323">
        <property type="protein sequence ID" value="ABF08323"/>
        <property type="gene ID" value="Rmet_1440"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1440"/>
    <dbReference type="eggNOG" id="COG0575">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037294_1_2_4"/>
    <dbReference type="OMA" id="WEWGRLN"/>
    <dbReference type="OrthoDB" id="1533756at2"/>
    <dbReference type="UniPathway" id="UPA00557">
        <property type="reaction ID" value="UER00614"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004605">
        <property type="term" value="F:phosphatidate cytidylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016024">
        <property type="term" value="P:CDP-diacylglycerol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000374">
        <property type="entry name" value="PC_trans"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01315">
        <property type="entry name" value="CDS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 9" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7 9" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 7 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="6"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="51"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="76"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="106"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="131"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="174"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="208"/>
            <end position="232"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003938"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ABF08323.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="273" mass="28697" checksum="73678D681E39187A" modified="2006-05-30" version="1">MLLTRVITAVCLLLLILPILFLAPPAALAGLVAVIVLLGGWEFGRLIGLRGAWPYVYAVACLLVLIGWHDAPQRDAVTWLLEASLIAWGVALVLMARGVRTATPAFTALGVVLGLIMLPAFGHAVMVLRAAGIGVLLTAAVLVWAADIGAYFVGKAIGKRKLAPTISPGKSWEGAIGGWLLAMIVALSLAATHTFAPTWYSLVADKGGLRLVVILTTLLVAASIVGDLFESLLKRQVGMKDSSRLLPGHGGILDRIDALIPVFPLAALLLAWI</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LNI5</accession>
    <name>Q1LNI5_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Putative peptide transporter subunit: periplasmic-binding component of ABC superfamily</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">dppA1b</name>
        <name evidence="5" type="ordered locus">Rmet_1408</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the bacterial solute-binding protein 5 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08291.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516164.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1408"/>
    <dbReference type="EnsemblBacteria" id="ABF08291">
        <property type="protein sequence ID" value="ABF08291"/>
        <property type="gene ID" value="Rmet_1408"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1408"/>
    <dbReference type="eggNOG" id="COG0747">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017028_7_3_4"/>
    <dbReference type="OMA" id="KESPWVP"/>
    <dbReference type="OrthoDB" id="162819at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043190">
        <property type="term" value="C:ATP-binding cassette (ABC) transporter complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030288">
        <property type="term" value="C:outer membrane-bounded periplasmic space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030678">
        <property type="entry name" value="Peptide/Ni-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039424">
        <property type="entry name" value="SBP_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023765">
        <property type="entry name" value="SBP_5_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000914">
        <property type="entry name" value="SBP_5_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30290">
        <property type="entry name" value="PTHR30290"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00496">
        <property type="entry name" value="SBP_bac_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002741">
        <property type="entry name" value="MppA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01040">
        <property type="entry name" value="SBP_BACTERIAL_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193642" evidence="3">
        <location>
            <begin position="31"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="domain" description="SBP_bac_5" evidence="4">
        <location>
            <begin position="75"/>
            <end position="432"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABF08291.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="518" mass="57349" checksum="FC94FD97F50E08DB" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LNK4</accession>
    <name>Q1LNK4_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">(S)-2-haloacid dehalogenase</fullName>
            <ecNumber evidence="2">3.8.1.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">2-haloalkanoic acid dehalogenase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="2">Halocarboxylic acid halidohydrolase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="2">L-2-haloacid dehalogenase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="3" type="primary">dehII</name>
        <name evidence="3" type="ordered locus">Rmet_1389</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Catalyzes the hydrolytic dehalogenation of small (S)-2-haloalkanoic acids to yield the corresponding (R)-2-hydroxyalkanoic acids.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>an (S)-2-haloacid + H2O = a (2R)-2-hydroxycarboxylate + a halide anion + H(+)</text>
            <dbReference type="Rhea" id="RHEA:11192"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16042"/>
            <dbReference type="ChEBI" id="CHEBI:58314"/>
            <dbReference type="ChEBI" id="CHEBI:137405"/>
            <dbReference type="EC" id="3.8.1.2"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the HAD-like hydrolase superfamily. S-2-haloalkanoic acid dehalogenase family.</text>
    </comment>
    <dbReference type="EC" id="3.8.1.2" evidence="2"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08272.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516148.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1389"/>
    <dbReference type="EnsemblBacteria" id="ABF08272">
        <property type="protein sequence ID" value="ABF08272"/>
        <property type="gene ID" value="Rmet_1389"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1389"/>
    <dbReference type="eggNOG" id="COG1011">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045011_3_1_4"/>
    <dbReference type="OMA" id="TTFWINR"/>
    <dbReference type="OrthoDB" id="1204073at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018784">
        <property type="term" value="F:(S)-2-haloacid dehalogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02588">
        <property type="entry name" value="HAD_L2-DEX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.240">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006328">
        <property type="entry name" value="2-HAD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006439">
        <property type="entry name" value="HAD-SF_hydro_IA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041492">
        <property type="entry name" value="HAD_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023198">
        <property type="entry name" value="PGP-like_dom2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13419">
        <property type="entry name" value="HAD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00413">
        <property type="entry name" value="HADHALOGNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="SSF56784"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01493">
        <property type="entry name" value="HAD-SF-IA-v2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01428">
        <property type="entry name" value="HAD_type_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU368077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF08272.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="237" mass="25915" checksum="0AE8F9D0A6AF8EEB" modified="2006-05-30" version="1">MSKIRAVVFDAYGTLFDVYSVTARAEQLFPGKGETLALLWRERQIDYSRLRSLAAPDGSRYKPFWDITIDALRYAAERLGLPLDEAAEAQLLKEYACLSAFPENLGALKRLRRANLPLGILSNGNPEMLDIAVKSAGMHGLFDHVLSVDPVRQYKTSPAAYALGPQAFNLSAGEILFVSSNCWDACGATWFGYTTFWINRAGHPAERLDVAPTGTGHDMNDLLAFASPLVTATGVAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="65" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LNS0</accession>
    <name>Q1LNS0_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_1323</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08206.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516089.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1323"/>
    <dbReference type="EnsemblBacteria" id="ABF08206">
        <property type="protein sequence ID" value="ABF08206"/>
        <property type="gene ID" value="Rmet_1323"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1323"/>
    <dbReference type="eggNOG" id="COG3193">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_103773_2_2_4"/>
    <dbReference type="OMA" id="MLPGKFA"/>
    <dbReference type="OrthoDB" id="1551899at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005624">
        <property type="entry name" value="PduO/GlcC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038084">
        <property type="entry name" value="PduO/GlcC-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03928">
        <property type="entry name" value="Haem_degrading"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143744">
        <property type="entry name" value="SSF143744"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF08206.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="149" mass="15614" checksum="F5CB0A9F818FC342" modified="2006-05-30" version="1">MTQADSLHSVEARLINWPAANRVVEAAALHAQRLGVCVNIAVVDVAGNLAAFLRMPGAPLHSIEIAIDKAYTAASFGLPTSRWSEALLSHSEAVRQGIVRRPRFIAFGGGLPIVESGHRIGGIGVSGGTEEQDEHCAQAGLSLLGLTQT</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LNS1</accession>
    <name>Q1LNS1_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">2-hydroxymuconic semialdehyde dehydrogenase (HMSD)(Protein belongs to CMGI-2)</fullName>
            <ecNumber evidence="6">1.2.1.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">dmpC</name>
        <name evidence="6" type="ordered locus">Rmet_1322</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the aldehyde dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.2.1.-" evidence="6"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08205.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516088.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1322"/>
    <dbReference type="EnsemblBacteria" id="ABF08205">
        <property type="protein sequence ID" value="ABF08205"/>
        <property type="gene ID" value="Rmet_1322"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1322"/>
    <dbReference type="eggNOG" id="COG1012">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005391_0_2_4"/>
    <dbReference type="OMA" id="RAHNFSQ"/>
    <dbReference type="OrthoDB" id="744602at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016620">
        <property type="term" value="F:oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.309.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.605.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016161">
        <property type="entry name" value="Ald_DH/histidinol_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016163">
        <property type="entry name" value="Ald_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016160">
        <property type="entry name" value="Ald_DH_CS_CYS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029510">
        <property type="entry name" value="Ald_DH_CS_GLU"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016162">
        <property type="entry name" value="Ald_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015590">
        <property type="entry name" value="Aldehyde_DH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017628">
        <property type="entry name" value="OHmuconic_semiald_DH"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00171">
        <property type="entry name" value="Aldedh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53720">
        <property type="entry name" value="SSF53720"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03216">
        <property type="entry name" value="OH_muco_semi_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00070">
        <property type="entry name" value="ALDEHYDE_DEHYDR_CYS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00687">
        <property type="entry name" value="ALDEHYDE_DEHYDR_GLU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 4 6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aldedh" evidence="5">
        <location>
            <begin position="36"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="active site" evidence="2">
        <location>
            <position position="271"/>
        </location>
    </feature>
    <feature type="active site" evidence="3">
        <location>
            <position position="305"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABF08205.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="503" mass="53736" checksum="C96F05E26AF1E660" modified="2006-05-30" version="1">MLITDPQATASTGNQSGARAKEIHNFINGEYVPGQRWFEKRSPLNNAVIAKVAEAGRAEVDAAVAAAQAALKGAWGRMSLAQRVEVLYAVADGINRRFDDFLAAEVEDTGKPMSLARHVDIPRGAANFKIFADVVKNVPTEFFEMPTPDGVGAINYAMRRPVGVVGVICPWNLPLLLMTWKVGPALACGNTVVVKPSEETPQTAALLGEVMNAAGVPRGVYNVVHGFGPNSTGEFLTTHRGINAITFTGETRTGAAIMKAAADGARPVSLEMGGKNAAIVFADCDFDAAIEGTLRSCFANCGQVCLGTERVYVERPIFDRFVSALKKGAEGMQLGRPEDPATGMGPLISQEHRDKVLSYYKKAVEAGATVVAGGGVPDMPDALKGGAWVQPTIWTGLGDDSVIAREEIFGPCVLVMPFDSEEEVIRRANDNDYGLATAIWTTNLSRAHRVAGAIEVGIAWVNSWFLRDLRTPFGGAKQSGIGREGGVHSLEFYTELKNVCIKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="49" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LNZ1</accession>
    <name>Q1LNZ1_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Putative lipoprotein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_1250</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08135.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1250"/>
    <dbReference type="EnsemblBacteria" id="ABF08135">
        <property type="protein sequence ID" value="ABF08135"/>
        <property type="gene ID" value="Rmet_1250"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1250"/>
    <dbReference type="eggNOG" id="COG3616">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_119528_0_0_4"/>
    <dbReference type="OMA" id="VGPHQQP"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021341">
        <property type="entry name" value="DUF2958"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11171">
        <property type="entry name" value="DUF2958"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF08135.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="193" mass="21095" checksum="D89B7A3968C63F4C" modified="2006-05-30" version="1">MKNQDDIPLRPIGLQSIVFIWRCYQCLCLYGNLHRRIPAKPRKRIGACAPRRKSGFAALRFGVQPHSCIIGFVGLPTMPLPLATAPERAQLLANGEARAAGRAIDPVPVVRLFTPDAHVTWLLAALDPADGDTAWGLIDLGIGMPAQGTVKLSDLAGIVGPHQQPVMRDLYFRPTRTLSEYTRLAERDGAIPD</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LP31</accession>
    <name>Q1LP31_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="9">ABC transporter-related protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">Rmet_1209</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08095.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515985.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1209"/>
    <dbReference type="EnsemblBacteria" id="ABF08095">
        <property type="protein sequence ID" value="ABF08095"/>
        <property type="gene ID" value="Rmet_1209"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1209"/>
    <dbReference type="eggNOG" id="COG1101">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_22_4"/>
    <dbReference type="OMA" id="VMLHQGN"/>
    <dbReference type="OrthoDB" id="1179151at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="1" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-1278">Translocase</keyword>
    <feature type="domain" description="ABC transporter" evidence="8">
        <location>
            <begin position="2"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="39"/>
            <end position="46"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ABF08095.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="264" mass="29100" checksum="CC26415A1D682DD1" modified="2006-05-30" version="1">MLRAENLKLTFNPGTPIENRALRGLSLEIPAGQFVAVIGSNGAGKSTFLNAVSGDLMIDTGRIMIDDADVTRKPAWDRAHLVARVFQDPMAGTCEALTIEENMALAMARGSRRGFRPALNKSSRELFRDKLRLLNLGLENRLTDRIGLLSGGQRQAVSLLMASLQPSRILLLDEHTAALDPKTAAFVLELTARIVEESKLTTMMVTHSMRQALDYGQRTVMLHQGQVVLDVSGDQRKGLDVPDLLKMFEQTRHEQLDDDALLLG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-12-12" modified="2022-08-03" version="95" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LP48</accession>
    <name>FOLD_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Bifunctional protein FolD</fullName>
        </recommendedName>
        <domain>
            <recommendedName>
                <fullName evidence="1">Methylenetetrahydrofolate dehydrogenase</fullName>
                <ecNumber evidence="1">1.5.1.5</ecNumber>
            </recommendedName>
        </domain>
        <domain>
            <recommendedName>
                <fullName evidence="1">Methenyltetrahydrofolate cyclohydrolase</fullName>
                <ecNumber evidence="1">3.5.4.9</ecNumber>
            </recommendedName>
        </domain>
    </protein>
    <gene>
        <name evidence="1" type="primary">folD</name>
        <name type="ordered locus">Rmet_1192</name>
    </gene>
    <organism>
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the oxidation of 5,10-methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate and then the hydrolysis of 5,10-methenyltetrahydrofolate to 10-formyltetrahydrofolate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(6R)-5,10-methylene-5,6,7,8-tetrahydrofolate + NADP(+) = 5,10-methenyltetrahydrofolate + NADPH</text>
            <dbReference type="Rhea" id="RHEA:22812"/>
            <dbReference type="ChEBI" id="CHEBI:15636"/>
            <dbReference type="ChEBI" id="CHEBI:57455"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.5.1.5"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>5,10-methenyltetrahydrofolate + H2O = (6S)-10-formyltetrahydrofolate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:23700"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57454"/>
            <dbReference type="ChEBI" id="CHEBI:57455"/>
            <dbReference type="EC" id="3.5.4.9"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">One-carbon metabolism; tetrahydrofolate interconversion.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family.</text>
    </comment>
    <dbReference type="EC" id="1.5.1.5" evidence="1"/>
    <dbReference type="EC" id="3.5.4.9" evidence="1"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08078.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515972.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q1LP48"/>
    <dbReference type="SMR" id="Q1LP48"/>
    <dbReference type="STRING" id="266264.Rmet_1192"/>
    <dbReference type="EnsemblBacteria" id="ABF08078">
        <property type="protein sequence ID" value="ABF08078"/>
        <property type="gene ID" value="Rmet_1192"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1192"/>
    <dbReference type="eggNOG" id="COG0190">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_034045_2_1_4"/>
    <dbReference type="OMA" id="HIVGRPM"/>
    <dbReference type="OrthoDB" id="1449514at2"/>
    <dbReference type="UniPathway" id="UPA00193"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004477">
        <property type="term" value="F:methenyltetrahydrofolate cyclohydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004488">
        <property type="term" value="F:methylenetetrahydrofolate dehydrogenase (NADP+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000105">
        <property type="term" value="P:histidine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009086">
        <property type="term" value="P:methionine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006164">
        <property type="term" value="P:purine nucleotide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035999">
        <property type="term" value="P:tetrahydrofolate interconversion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd01080">
        <property type="entry name" value="NAD_bind_m-THF_DH_Cyclohyd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01576">
        <property type="entry name" value="THF_DHG_CYH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046346">
        <property type="entry name" value="Aminiacid_DH-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000672">
        <property type="entry name" value="THF_DH/CycHdrlase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020630">
        <property type="entry name" value="THF_DH/CycHdrlase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020867">
        <property type="entry name" value="THF_DH/CycHdrlase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020631">
        <property type="entry name" value="THF_DH/CycHdrlase_NAD-bd_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00763">
        <property type="entry name" value="THF_DHG_CYH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02882">
        <property type="entry name" value="THF_DHG_CYH_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00085">
        <property type="entry name" value="THFDHDRGNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53223">
        <property type="entry name" value="SSF53223"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00766">
        <property type="entry name" value="THF_DHG_CYH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00767">
        <property type="entry name" value="THF_DHG_CYH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0368">Histidine biosynthesis</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0486">Methionine biosynthesis</keyword>
    <keyword id="KW-0511">Multifunctional enzyme</keyword>
    <keyword id="KW-0521">NADP</keyword>
    <keyword id="KW-0554">One-carbon metabolism</keyword>
    <keyword id="KW-0560">Oxidoreductase</keyword>
    <keyword id="KW-0658">Purine biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000268457" description="Bifunctional protein FolD">
        <location>
            <begin position="1"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="165"/>
            <end position="167"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="190"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01576"/>
        </source>
    </evidence>
    <sequence length="285" mass="30005" checksum="790F335E278AAA85" modified="2006-05-30" version="1">MSAHLIDGNALAKQIRAEAAQRAARLTAAGHQPGLAVVLVGEDPASQVYVRNKVKACEDNGFYSSLNRYPADLSEADLLARIDELNNDPKIHGILVQLPLPKHIDSHKVLEAIAPEKDVDGFHVANAGALMTGAPLFRPCTPYGCMKMLESIQYPLRGARAVVVGASNIVGKPMAMMLLQAGATVTICNSKTRDLAAHTRDADVIVAAVGRRNIITADIVKPGAVVIDVGMNRDDAGKLCGDVDFAGVREVAGHITPVPGGVGPMTITMLLINTLEAAEREAGIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="108" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LPF9</accession>
    <name>Q1LPF9_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">UDP-2,3-diacylglucosamine hydrolase</fullName>
            <ecNumber evidence="9">3.6.1.54</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">UDP-2,3-diacylglucosamine diphosphatase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9 11" type="primary">lpxH</name>
        <name evidence="11" type="ordered locus">Rmet_1081</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Hydrolyzes the pyrophosphate bond of UDP-2,3-diacylglucosamine to yield 2,3-diacylglucosamine 1-phosphate (lipid X) and UMP by catalyzing the attack of water at the alpha-P atom. Involved in the biosynthesis of lipid A, a phosphorylated glycolipid that anchors the lipopolysaccharide to the outer membrane of the cell.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>H2O + UDP-2-N,3-O-bis[(3R)-3-hydroxytetradecanoyl]-alpha-D-glucosamine = 2-N,3-O-bis[(3R)-3-hydroxytetradecanoyl]-alpha-D-glucosaminyl 1-phosphate + 2 H(+) + UMP</text>
            <dbReference type="Rhea" id="RHEA:25213"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57865"/>
            <dbReference type="ChEBI" id="CHEBI:57957"/>
            <dbReference type="ChEBI" id="CHEBI:78847"/>
            <dbReference type="EC" id="3.6.1.54"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="9">Binds 2 Mn(2+) ions per subunit in a binuclear metal center.</text>
    </comment>
    <comment type="pathway">
        <text evidence="9">Glycolipid biosynthesis; lipid IV(A) biosynthesis; lipid IV(A) from (3R)-3-hydroxytetradecanoyl-[acyl-carrier-protein] and UDP-N-acetyl-alpha-D-glucosamine: step 4/6.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cell inner membrane</location>
            <topology evidence="9">Peripheral membrane protein</topology>
            <orientation evidence="9">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the LpxH family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="3.6.1.54" evidence="9"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07967.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515869.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1081"/>
    <dbReference type="EnsemblBacteria" id="ABF07967">
        <property type="protein sequence ID" value="ABF07967"/>
        <property type="gene ID" value="Rmet_1081"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1081"/>
    <dbReference type="eggNOG" id="COG2908">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_074586_0_0_4"/>
    <dbReference type="OMA" id="FDFWFEY"/>
    <dbReference type="OrthoDB" id="1690667at2"/>
    <dbReference type="UniPathway" id="UPA00359">
        <property type="reaction ID" value="UER00480"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019897">
        <property type="term" value="C:extrinsic component of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030145">
        <property type="term" value="F:manganese ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008758">
        <property type="term" value="F:UDP-2,3-diacylglucosamine hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009245">
        <property type="term" value="P:lipid A biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00575">
        <property type="entry name" value="LpxH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP_ApaH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043461">
        <property type="entry name" value="LpxH-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010138">
        <property type="entry name" value="UDP-diacylglucosamine_Hdrlase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34990">
        <property type="entry name" value="PTHR34990"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34990:SF1">
        <property type="entry name" value="PTHR34990:SF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="SSF56300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01854">
        <property type="entry name" value="lipid_A_lpxH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 9" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="1 9" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9 11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 9" id="KW-0441">Lipid A biosynthesis</keyword>
    <keyword evidence="2 9" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="6 9" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="8 9" id="KW-0464">Manganese</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Metallophos" evidence="10">
        <location>
            <begin position="20"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="9">
        <location>
            <begin position="96"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="24"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="26"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="57"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="96"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="131"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="139"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="177"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="184"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="217"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="219"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022556"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00575"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ABF07967.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="263" mass="29098" checksum="F129CF228F4743DE" modified="2006-05-30" version="1">MTAPTRTPVAAPIEVQAPAWFISDLHLTADMPRTLAAFHRVLERAARDARTLFILGDFFEFWVGDEETDSPFAAEVAAALRRLADAGVPVYLMHGNRDFLLGRRFAARAGASLLPDPSVIVCAGRRVALSHGDMLCTDDERYMRFRRWTRKGWVQRLFLLLPLGTRLSIARRLRADSEAGRTRQDIDAAPVTYGDAAPDAVNALLASSGAGTLVHGHTHHPARHTHEAGERWVLTDWDLDGRHPRAAVLELDGTGFRVLPQVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LPH5</accession>
    <name>Q1LPH5_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative translation factor (SUA5)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_1065</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07951.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515853.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1065"/>
    <dbReference type="EnsemblBacteria" id="ABF07951">
        <property type="protein sequence ID" value="ABF07951"/>
        <property type="gene ID" value="Rmet_1065"/>
    </dbReference>
    <dbReference type="GeneID" id="60821926"/>
    <dbReference type="KEGG" id="rme:Rmet_1065"/>
    <dbReference type="eggNOG" id="COG0009">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031397_3_0_4"/>
    <dbReference type="OMA" id="MLYPTDT"/>
    <dbReference type="OrthoDB" id="1913202at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003725">
        <property type="term" value="F:double-stranded RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017945">
        <property type="entry name" value="DHBP_synth_RibB-like_a/b_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006070">
        <property type="entry name" value="YrdC-like_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01300">
        <property type="entry name" value="Sua5_yciO_yrdC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55821">
        <property type="entry name" value="SSF55821"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00057">
        <property type="entry name" value="TIGR00057"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51163">
        <property type="entry name" value="YRDC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="YrdC-like" evidence="1">
        <location>
            <begin position="14"/>
            <end position="200"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF07951.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="215" mass="23624" checksum="A86EE95AC28178E0" modified="2006-05-30" version="1">MSQYFEIHPDNPQARLVKQAAQIVRQGGLIALPTDSSYALACQIDDKAAVEQLRRLREIDEKHHLTLMCSDLSELGNFARVDNRQYRWIKGATPGPYVFILEATKEVPRRLSHPARKTIGVRVPEHPIPLALVAELGQPLISATLQLPGDDAPMNDPAEIRARLEKRLDLVIDGGPAPAQPTTVIDLTSGEPVLVRAGRGDVERFGLNASDFATT</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="66" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LPI3</accession>
    <name>Q1LPI3_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_1057</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07943.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515846.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1057"/>
    <dbReference type="EnsemblBacteria" id="ABF07943">
        <property type="protein sequence ID" value="ABF07943"/>
        <property type="gene ID" value="Rmet_1057"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1057"/>
    <dbReference type="HOGENOM" id="CLU_149883_0_0_4"/>
    <dbReference type="OMA" id="GWGGGHW"/>
    <dbReference type="OrthoDB" id="2098179at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193400" evidence="2">
        <location>
            <begin position="26"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="35"/>
            <end position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF07943.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="131" mass="14391" checksum="C845AF2B981A47C9" modified="2006-05-30" version="1">MTKTYRSRALPWLVIAMAAMITGCAAPVPRDMYGSPATARLEPGSTPPAPLTDEEKQQLTELNQQVLREQEHVIASQQQAEAWARAAYAYPSTNWNLYYGGWGGGHWGGGVGISSPGWGWGGYPYGSPYWW</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="61" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LPK1</accession>
    <name>Q1LPK1_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_1039</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07925.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515830.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1039"/>
    <dbReference type="EnsemblBacteria" id="ABF07925">
        <property type="protein sequence ID" value="ABF07925"/>
        <property type="gene ID" value="Rmet_1039"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1039"/>
    <dbReference type="eggNOG" id="COG4255">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037503_1_0_4"/>
    <dbReference type="OMA" id="GHNIDIW"/>
    <dbReference type="OrthoDB" id="1293048at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016631">
        <property type="entry name" value="Regulatory_RpfE"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015283">
        <property type="entry name" value="Regulatory_RpfE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF07925.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="372" mass="40396" checksum="3D16C1B089391A97" modified="2006-05-30" version="1">MMTHLTLIVPFSVPPGAGDDASDDVVPGALLRQLELPALGKLLTRATPGPRETHDDPYLRTLPQERWLAGRAGLDTTRVPTAPFMRLADHRGATPPPTQAAGEGGWACLQPVHIHAARDHLVLMHPDQLDIRPDETAALHAAIAGLLDEAGITIDTPHPARWYIPDGMFGDLTATTPVRATGRNIDIWMQAGPKARDWRRLQNEIQMTWHGHPVNEAREAEGRLTVNSVWLHGGGRANAARKLADTVLTDDPFLAGLGLAGGSRLGPQPARFADIADTDGSVMTLLDSATEAHIAADWGLWLERMHTLDAEWFAPVLDALGEGRIQAVTLVLGGENHFAEFTVTRADLRKFWRGLGSRGDWRALLSDLAMVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="96" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LPL2</accession>
    <name>Q1LPL2_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Co-chaperone protein HscB homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4 6" type="primary">hscB</name>
        <name evidence="6" type="ordered locus">Rmet_1028</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3 4">Co-chaperone involved in the maturation of iron-sulfur cluster-containing proteins. Seems to help targeting proteins to be folded toward HscA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Interacts with HscA and stimulates its ATPase activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the HscB family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07914.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515821.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1028"/>
    <dbReference type="EnsemblBacteria" id="ABF07914">
        <property type="protein sequence ID" value="ABF07914"/>
        <property type="gene ID" value="Rmet_1028"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1028"/>
    <dbReference type="eggNOG" id="COG1076">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_068529_2_0_4"/>
    <dbReference type="OMA" id="FIARFAH"/>
    <dbReference type="OrthoDB" id="1520143at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001671">
        <property type="term" value="F:ATPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051087">
        <property type="term" value="F:chaperone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044571">
        <property type="term" value="P:[2Fe-2S] cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051259">
        <property type="term" value="P:protein complex oligomerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00682">
        <property type="entry name" value="HscB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004640">
        <property type="entry name" value="HscB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036386">
        <property type="entry name" value="HscB_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009073">
        <property type="entry name" value="HscB_oligo_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14021">
        <property type="entry name" value="PTHR14021"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07743">
        <property type="entry name" value="HSCB_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="SSF46565"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47144">
        <property type="entry name" value="SSF47144"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00714">
        <property type="entry name" value="hscB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0143">Chaperone</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="J" evidence="5">
        <location>
            <begin position="4"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010476"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00025596"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABF07914.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="172" mass="19506" checksum="85000DBB4A3BC4EB" modified="2006-05-30" version="1">MKDDFFALFGLPARYEVDEAALDAAYRTVQSQAHPDRFANAGDAERRVAMQWAAHANEAYRTLRQPLKRAIYLLHLRGVDIQAESNTAMAPEFLMQQMEWREALQDAVDGRAVDQLDRLLRDLRQEKLERHAALGALLDADDNGAAGEAARQLMFIEKIEHDASEAIDRLEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LPM9</accession>
    <name>Q1LPM9_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Alpha/beta hydrolase superfamily</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_1011</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07897.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515810.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1011"/>
    <dbReference type="ESTHER" id="ralme-q1lpm9">
        <property type="family name" value="6_AlphaBeta_hydrolase"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF07897">
        <property type="protein sequence ID" value="ABF07897"/>
        <property type="gene ID" value="Rmet_1011"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1011"/>
    <dbReference type="eggNOG" id="COG2267">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020336_13_0_4"/>
    <dbReference type="OMA" id="HGYSERG"/>
    <dbReference type="OrthoDB" id="1119700at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00561">
        <property type="entry name" value="Abhydrolase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00111">
        <property type="entry name" value="ABHYDROLASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="SSF53474"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="domain" description="AB hydrolase-1" evidence="2">
        <location>
            <begin position="60"/>
            <end position="169"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF07897.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="322" mass="34317" checksum="23DE3137C55849D6" modified="2006-05-30" version="1">MNALLSVVVAVVGLLVLIGLALFLYTWRTTRKIEAALPPTGRFIDVPGAQLHVVERGTGPAILLVHGLSGQLGNFDYGMIEPLARDFRVVAIDRPAAGYSTRAPGTPADLPAQADTLAALIDKLGLDKPLVVGHSLGGAIALTLASRHPERVGGLALIAPLTHPPEHISPVFEAMTIPSAWKRKLLAWTLLIPMSIRNREQVMDIVFGPDRAPDDFPMRGGGLLAMRPSHFLGASEDLIGAASSLPAVVPHYGTLRMPIHVLYGREDRILDYKLNGEAFAAKVPGARLTLVSGGHMLPVTAIDTSVDFVRNAALQLHEPVSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="95" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LPP2</accession>
    <name>Q1LPP2_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 5">DNA repair protein RecN</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4 5">Recombination protein N</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">recN</name>
        <name evidence="8" type="ordered locus">Rmet_0998</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 5">May be involved in recombinational repair of damaged DNA.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the RecN family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07884.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515799.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0998"/>
    <dbReference type="EnsemblBacteria" id="ABF07884">
        <property type="protein sequence ID" value="ABF07884"/>
        <property type="gene ID" value="Rmet_0998"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0998"/>
    <dbReference type="eggNOG" id="COG0497">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018297_3_1_4"/>
    <dbReference type="OMA" id="QVICVTH"/>
    <dbReference type="OrthoDB" id="1074831at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004604">
        <property type="entry name" value="DNA_recomb/repair_RecN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003395">
        <property type="entry name" value="RecF/RecN/SMC_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11059">
        <property type="entry name" value="PTHR11059"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02463">
        <property type="entry name" value="SMC_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF003128">
        <property type="entry name" value="RecN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00634">
        <property type="entry name" value="recN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0227">DNA damage</keyword>
    <keyword evidence="5" id="KW-0234">DNA repair</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SMC_N" evidence="7">
        <location>
            <begin position="2"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="507"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="562"/>
            <end position="589"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00021315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00033408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRNR" id="PIRNR003128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF02463"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ABF07884.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="589" mass="63120" checksum="2BBD939192D296F5" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="101" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LPX9</accession>
    <name>Q1LPX9_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 8">Acetolactate synthase</fullName>
            <ecNumber evidence="4 8">2.2.1.6</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">ilvI</name>
        <name evidence="12" type="ordered locus">Rmet_0911</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>H(+) + 2 pyruvate = (2S)-2-acetolactate + CO2</text>
            <dbReference type="Rhea" id="RHEA:25249"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:58476"/>
            <dbReference type="EC" id="2.2.1.6"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="8">Binds 1 Mg(2+) ion per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>thiamine diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58937"/>
        </cofactor>
        <text evidence="8">Binds 1 thiamine pyrophosphate per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1 8">Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 1/4.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 8">Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 1/4.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 8">Belongs to the TPP enzyme family.</text>
    </comment>
    <dbReference type="EC" id="2.2.1.6" evidence="4 8"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07797.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008643327.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0911"/>
    <dbReference type="EnsemblBacteria" id="ABF07797">
        <property type="protein sequence ID" value="ABF07797"/>
        <property type="gene ID" value="Rmet_0911"/>
    </dbReference>
    <dbReference type="GeneID" id="60825399"/>
    <dbReference type="KEGG" id="rme:Rmet_0911"/>
    <dbReference type="eggNOG" id="COG0028">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013748_1_2_4"/>
    <dbReference type="OMA" id="CFGTSGP"/>
    <dbReference type="OrthoDB" id="391134at2"/>
    <dbReference type="UniPathway" id="UPA00047">
        <property type="reaction ID" value="UER00055"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00049">
        <property type="reaction ID" value="UER00059"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003984">
        <property type="term" value="F:acetolactate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030976">
        <property type="term" value="F:thiamine pyrophosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009097">
        <property type="term" value="P:isoleucine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009099">
        <property type="term" value="P:valine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd02015">
        <property type="entry name" value="TPP_AHAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012846">
        <property type="entry name" value="Acetolactate_synth_lsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039368">
        <property type="entry name" value="AHAS_TPP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029035">
        <property type="entry name" value="DHS-like_NAD/FAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012000">
        <property type="entry name" value="Thiamin_PyroP_enz_cen_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012001">
        <property type="entry name" value="Thiamin_PyroP_enz_TPP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045229">
        <property type="entry name" value="TPP_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011766">
        <property type="entry name" value="TPP_enzyme-bd_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18968">
        <property type="entry name" value="PTHR18968"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02775">
        <property type="entry name" value="TPP_enzyme_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00205">
        <property type="entry name" value="TPP_enzyme_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02776">
        <property type="entry name" value="TPP_enzyme_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52467">
        <property type="entry name" value="SSF52467"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="SSF52518"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00118">
        <property type="entry name" value="acolac_lg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="7 8" id="KW-0100">Branched-chain amino acid biosynthesis</keyword>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0786">Thiamine pyrophosphate</keyword>
    <keyword evidence="6 8 12" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="TPP_enzyme_N" evidence="11">
        <location>
            <begin position="23"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="domain" description="TPP_enzyme_M" evidence="9">
        <location>
            <begin position="211"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="domain" description="TPP_enzyme_C" evidence="10">
        <location>
            <begin position="410"/>
            <end position="558"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007812"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003591"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF02775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF02776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ABF07797.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="587" mass="64784" checksum="E6A2BA6B754023EB" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="72" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LQB8</accession>
    <name>Q1LQB8_CUPMC</name>
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        <recommendedName>
            <fullName evidence="1">HD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_0772</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07658.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515613.1">
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    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0772"/>
    <dbReference type="EnsemblBacteria" id="ABF07658">
        <property type="protein sequence ID" value="ABF07658"/>
        <property type="gene ID" value="Rmet_0772"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0772"/>
    <dbReference type="eggNOG" id="COG4341">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_091985_0_1_4"/>
    <dbReference type="OMA" id="QHSLQCA"/>
    <dbReference type="OrthoDB" id="1704279at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd00077">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003607">
        <property type="entry name" value="HD/PDEase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006674">
        <property type="entry name" value="HD_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017670">
        <property type="entry name" value="Phosphonate_degrad_HDIG"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01966">
        <property type="entry name" value="HD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03276">
        <property type="entry name" value="Phn-HD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HD" evidence="1">
        <location>
            <begin position="31"/>
            <end position="87"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF07658.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="179" mass="19150" checksum="D22488DDBA6CB33A" modified="2006-05-30" version="1">MTTLTLSSIAALFENHGTAYYGGEAISQTAHALQCALLAEQAGDPEPLIVAALLHDIGHLMLAESLTTDMRHQETGADALASLFGEDVTEPVRMHVAAKRYLCAVDPAYFDTLSPVSVHSLSLQGGPYDGEQASVFAAQAHADAAVRLRRYDDLAKVVDLQTPTLSHYLDMAARCVRVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="103" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LQD6</accession>
    <name>Q1LQD6_CUPMC</name>
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        <recommendedName>
            <fullName evidence="1">DNA-directed DNA polymerase</fullName>
            <ecNumber evidence="1">2.7.7.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">dnaE</name>
        <name evidence="8" type="ordered locus">Rmet_0754</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
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        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) = diphosphate + DNA(n+1)</text>
            <dbReference type="Rhea" id="RHEA:22508"/>
            <dbReference type="Rhea" id="RHEA-COMP:17339"/>
            <dbReference type="Rhea" id="RHEA-COMP:17340"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61560"/>
            <dbReference type="ChEBI" id="CHEBI:173112"/>
            <dbReference type="EC" id="2.7.7.7"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.7.7.7" evidence="1"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07640.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0754"/>
    <dbReference type="EnsemblBacteria" id="ABF07640">
        <property type="protein sequence ID" value="ABF07640"/>
        <property type="gene ID" value="Rmet_0754"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0754"/>
    <dbReference type="eggNOG" id="COG0587">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001600_0_0_4"/>
    <dbReference type="OMA" id="NECRRMG"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008408">
        <property type="term" value="F:3'-5' exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003887">
        <property type="term" value="F:DNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="DNA_pol3_alpha_thumb_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040982">
        <property type="entry name" value="DNA_pol3_finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029460">
        <property type="entry name" value="DNAPol_HHH"/>
    </dbReference>
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        <property type="entry name" value="PHP_dom"/>
    </dbReference>
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        <property type="entry name" value="Pol/His_phosphatase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016195">
        <property type="entry name" value="Pol/histidinol_Pase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004805">
        <property type="entry name" value="PolC_alpha"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32294">
        <property type="entry name" value="PTHR32294"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="DNA_pol3_alpha"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00481">
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="5" id="KW-0239">DNA-directed DNA polymerase</keyword>
    <keyword evidence="3 8" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="POLIIIAc" evidence="7">
        <location>
            <begin position="16"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00024632"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ABF07640.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="1176" mass="130447" checksum="8C92234569DCB8FB" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LQE2</accession>
    <name>Q1LQE2_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">30S ribosomal protein S16</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3 4" type="primary">rpsP</name>
        <name evidence="4" type="ordered locus">Rmet_0748</name>
    </gene>
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        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the bacterial ribosomal protein bS16 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07634.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0748"/>
    <dbReference type="EnsemblBacteria" id="ABF07634">
        <property type="protein sequence ID" value="ABF07634"/>
        <property type="gene ID" value="Rmet_0748"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0748"/>
    <dbReference type="eggNOG" id="COG0228">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_100590_5_1_4"/>
    <dbReference type="OMA" id="GFYNPIA"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1320.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00385">
        <property type="entry name" value="Ribosomal_S16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000307">
        <property type="entry name" value="Ribosomal_S16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023803">
        <property type="entry name" value="Ribosomal_S16_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12919">
        <property type="entry name" value="PTHR12919"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00886">
        <property type="entry name" value="Ribosomal_S16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54565">
        <property type="entry name" value="SSF54565"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00002">
        <property type="entry name" value="S16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="1 3" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF07634.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="109" mass="12389" checksum="65A3208417E97A8B" modified="2006-05-30" version="1">MFVWAREVGYTLAFRVRTLPFFRGINMVVIRLARGGSKKRPFFNIVATDSRNRRDGRFIERIGFYNPLASEKEEGLRVVQDRLAYWQGVGAQLSPTVARLVKQAGKAAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="66" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LQM7</accession>
    <name>Q1LQM7_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">TPM_phosphatase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Rmet_0663</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07549.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515520.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0663"/>
    <dbReference type="EnsemblBacteria" id="ABF07549">
        <property type="protein sequence ID" value="ABF07549"/>
        <property type="gene ID" value="Rmet_0663"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0663"/>
    <dbReference type="eggNOG" id="COG3762">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_086382_1_0_4"/>
    <dbReference type="OMA" id="HTGQIRI"/>
    <dbReference type="OrthoDB" id="1927539at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007621">
        <property type="entry name" value="TPM_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04536">
        <property type="entry name" value="TPM_phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="TPM_phosphatase" evidence="4">
        <location>
            <begin position="33"/>
            <end position="152"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF04536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABF07549.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="178" mass="19806" checksum="2DA97324CDBDB95F" modified="2006-05-30" version="1">MKKASHPRPPFHQSLKRALRHIGTGHSVARRAFPDEARHQLQVAVHTGEQHHRGEVRVVIEANLPLSLAWRGVTPRARARTLFGALEVWNTEDHTGVLLYINLADHAVELLADRGIDARVKPEAWHDICAHLAQGLARNVSVEPVLEAIAQIHTLLTRHFPASGGNNPNELDDRPIVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="109" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LQN1</accession>
    <name>Q1LQN1_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains Sigma-54 dependent DNA-binding transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Rmet_0659</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07545.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515516.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0659"/>
    <dbReference type="EnsemblBacteria" id="ABF07545">
        <property type="protein sequence ID" value="ABF07545"/>
        <property type="gene ID" value="Rmet_0659"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0659"/>
    <dbReference type="eggNOG" id="COG2204">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_0_6_4"/>
    <dbReference type="OMA" id="GDMPFES"/>
    <dbReference type="OrthoDB" id="123059at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002197">
        <property type="entry name" value="HTH_Fis"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002078">
        <property type="entry name" value="Sigma_54_int"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025943">
        <property type="entry name" value="Sigma_54_int_dom_ATP-bd_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025944">
        <property type="entry name" value="Sigma_54_int_dom_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045343">
        <property type="entry name" value="VpsR"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02954">
        <property type="entry name" value="HTH_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00158">
        <property type="entry name" value="Sigma54_activat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20161">
        <property type="entry name" value="VpsR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00676">
        <property type="entry name" value="SIGMA54_INTERACT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00688">
        <property type="entry name" value="SIGMA54_INTERACT_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50045">
        <property type="entry name" value="SIGMA54_INTERACT_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Sigma-54 factor interaction" evidence="6">
        <location>
            <begin position="144"/>
            <end position="373"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50045"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABF07545.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="458" mass="50603" checksum="0D072AF01A25AEC9" modified="2006-05-30" version="1">MSHYVNRPLLYVSRHHDASLCRYLSQHGWKLVFANSLEDLAALPLDGPFSAGLLDFQSGFSDTDLELMEPWLATRQIGWIGAISCADELTDTARKLLGLYFIDYYTAPFDMAELSRTLGHAVGMAALRPGREQPAVPLTRPHGIIGECNAMQALFRSIEKVSRWDTPVLISGESGTGKELAAQAIHRASRRANQPFVAVNCAAIPPTLLMSELFGYERGAFTGATKRKPGRIEIAQGGTLFLDEIGDMPLESQTSLLRFLETGRIERLGGTESVAVDVRVISATHVDLEAAVATERFRGDLFHRLCVLRVKQPPLRERGSDVLLLAEHILDSVRKDSPEHIRGFSPMALRAITTHAWPGNVRELLNRIRLAAAMCENGVIQPSDLELDTPVESKPPTLAAIREAAEQAAILEALQRHHERLIDVAAELGISRVTLFRLMRDYKLQVRKGNVGLVCVQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="65" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LQW1</accession>
    <name>Q1LQW1_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Paraquat-inducible protein B</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">pqiB</name>
        <name evidence="4" type="ordered locus">Rmet_0579</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07465.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515440.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0579"/>
    <dbReference type="EnsemblBacteria" id="ABF07465">
        <property type="protein sequence ID" value="ABF07465"/>
        <property type="gene ID" value="Rmet_0579"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0579"/>
    <dbReference type="eggNOG" id="COG1463">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG3008">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018765_3_0_4"/>
    <dbReference type="OMA" id="TADTRFW"/>
    <dbReference type="OrthoDB" id="855063at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003399">
        <property type="entry name" value="Mce/MlaD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02470">
        <property type="entry name" value="MlaD"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="31"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="domain" description="MlaD" evidence="3">
        <location>
            <begin position="55"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="domain" description="MlaD" evidence="3">
        <location>
            <begin position="170"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="domain" description="MlaD" evidence="3">
        <location>
            <begin position="303"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF02470"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF07465.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="546" mass="59623" checksum="C7092C0EB27D1676" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LR27</accession>
    <name>Q1LR27_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Multidrug efflux system, subunit B</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">mdtB</name>
        <name evidence="3" type="ordered locus">Rmet_0513</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07399.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515379.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0513"/>
    <dbReference type="EnsemblBacteria" id="ABF07399">
        <property type="protein sequence ID" value="ABF07399"/>
        <property type="gene ID" value="Rmet_0513"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0513"/>
    <dbReference type="eggNOG" id="COG0841">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002755_1_2_4"/>
    <dbReference type="OMA" id="FIIPCFY"/>
    <dbReference type="OrthoDB" id="145346at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2090.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027463">
        <property type="entry name" value="AcrB_DN_DC_subdom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001036">
        <property type="entry name" value="Acrflvin-R"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32063">
        <property type="entry name" value="PTHR32063"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00873">
        <property type="entry name" value="ACR_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00702">
        <property type="entry name" value="ACRIFLAVINRP"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82714">
        <property type="entry name" value="SSF82714"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="336"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="360"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="431"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="463"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="528"/>
            <end position="548"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="857"/>
            <end position="875"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="908"/>
            <end position="933"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="953"/>
            <end position="974"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="986"/>
            <end position="1012"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1028"/>
            <end position="1051"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF07399.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="1051" mass="112905" checksum="40301D837E1FE1D1" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="107" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LRA2</accession>
    <name>Q1LRA2_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">4-hydroxythreonine-4-phosphate dehydrogenase</fullName>
            <ecNumber evidence="4">1.1.1.262</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">4-(phosphohydroxy)-L-threonine dehydrogenase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="4 5" type="primary">pdxA</name>
        <name evidence="5" type="ordered locus">Rmet_0438</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Catalyzes the NAD(P)-dependent oxidation of 4-(phosphooxy)-L-threonine (HTP) into 2-amino-3-oxo-4-(phosphooxy)butyric acid which spontaneously decarboxylates to form 3-amino-2-oxopropyl phosphate (AHAP).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>4-(phosphooxy)-L-threonine + NAD(+) = 3-amino-2-oxopropyl phosphate + CO2 + NADH</text>
            <dbReference type="Rhea" id="RHEA:32275"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57279"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:58452"/>
            <dbReference type="EC" id="1.1.1.262"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="4">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <cofactor evidence="4">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <cofactor evidence="4">
            <name>Co(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:48828"/>
        </cofactor>
        <text evidence="4">Binds 1 divalent metal cation per subunit. Can use ions such as Zn(2+), Mg(2+) or Co(2+).</text>
    </comment>
    <comment type="pathway">
        <text evidence="4">Cofactor biosynthesis; pyridoxine 5'-phosphate biosynthesis; pyridoxine 5'-phosphate from D-erythrose 4-phosphate: step 4/5.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="miscellaneous">
        <text evidence="4">The active site is located at the dimer interface.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the PdxA family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.262" evidence="4"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07324.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515312.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0438"/>
    <dbReference type="EnsemblBacteria" id="ABF07324">
        <property type="protein sequence ID" value="ABF07324"/>
        <property type="gene ID" value="Rmet_0438"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0438"/>
    <dbReference type="eggNOG" id="COG1995">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_040168_1_0_4"/>
    <dbReference type="OMA" id="TAQVVMM"/>
    <dbReference type="OrthoDB" id="1414545at2"/>
    <dbReference type="UniPathway" id="UPA00244">
        <property type="reaction ID" value="UER00312"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050570">
        <property type="term" value="F:4-hydroxythreonine-4-phosphate dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050897">
        <property type="term" value="F:cobalt ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042823">
        <property type="term" value="P:pyridoxal phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008615">
        <property type="term" value="P:pyridoxine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00536">
        <property type="entry name" value="PdxA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037510">
        <property type="entry name" value="PdxA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005255">
        <property type="entry name" value="PdxA_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30004">
        <property type="entry name" value="PTHR30004"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04166">
        <property type="entry name" value="PdxA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00557">
        <property type="entry name" value="pdxA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0170">Cobalt</keyword>
    <keyword evidence="1 4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0460">Magnesium</keyword>
    <keyword evidence="2 4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0520">NAD</keyword>
    <keyword evidence="4" id="KW-0521">NADP</keyword>
    <keyword evidence="3 4 5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-0664">Pyridoxine biosynthesis</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <feature type="binding site" evidence="4">
        <location>
            <position position="141"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="142"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="178"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="223"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="278"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="286"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="295"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="304"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABF07324.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="358" mass="37660" checksum="3E38FA8002BC9D02" modified="2006-05-30" version="1">MPDPAPLDLAITTGEPAGIGPDITIGALLQFAANHGGNVGHGGAVRFHVIGDARLLDQRAEALGVAHAWARRIADGDVIVEDIALGVHCEPGRLDARNGHYVLALLDAAVDGLRTGRYAAMITAPVQKSTINDAGVPFSGHTEYLAECAGVPRVVMMLAGPQPAHDNAMLRVALATTHLPLRAVPDALNIPMLVETLAIVDADLRRNFGIARPRILVTGLNPHAGESGHMGREEIDVISPALEQARDADIDARGPYPADTLFQPRHLRDADCVLAMYHDQGLAPLKYGTFGHGVNITLGLPFIRTSVDHGTALDLAGTGRAEHGSMIEAIRCAITMAGHASGRHSNGRADNGISNGQH</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="106" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LRD8</accession>
    <name>Q1LRD8_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Glutamate and aspartate transporter subunit ATP-binding component of ABC superfamily</fullName>
            <ecNumber evidence="10">3.6.3.28</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">gltL</name>
        <name evidence="10" type="ordered locus">Rmet_0402</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ABC transporter superfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.3.28" evidence="10"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07288.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008645995.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0402"/>
    <dbReference type="EnsemblBacteria" id="ABF07288">
        <property type="protein sequence ID" value="ABF07288"/>
        <property type="gene ID" value="Rmet_0402"/>
    </dbReference>
    <dbReference type="GeneID" id="60824004"/>
    <dbReference type="KEGG" id="rme:Rmet_0402"/>
    <dbReference type="eggNOG" id="COG1126">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_22_4"/>
    <dbReference type="OMA" id="CSQIGMV"/>
    <dbReference type="OrthoDB" id="1191583at2"/>
    <dbReference type="Proteomes" id="UP000002429">
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        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="term" value="F:ABC-type amino acid transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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        <property type="term" value="F:ATP binding"/>
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        <property type="project" value="UniProtKB-UniRule"/>
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        <property type="term" value="F:hydrolase activity"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="AAA+_ATPase"/>
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        <property type="entry name" value="ABC_ATPase_HisP-typ"/>
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    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF039085">
        <property type="entry name" value="ABC_ATPase_HisP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-1278">Translocase</keyword>
    <feature type="domain" description="ABC transporter" evidence="9">
        <location>
            <begin position="2"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="34"/>
            <end position="41"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ABF07288.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="244" mass="26889" checksum="E3359A9F48E17FF7" modified="2006-05-30" version="1">MIEINNVSKWYGAFQVLTDCTTKVAKGEVVVVCGPSGSGKSTLIKTVNALEPFQKGDILVDGTSVGNPKTNLPKLRSRVGMVFQNFELFPHLSITENLTIAQMKVLGRSKDEAMAKGLKYLERVGLKSQAEKYPGQLSGGQQQRVAIARALSMDPICMLFDEPTSALDPEMVNEVLDVMVQLAQEGMTMMCVTHEMGFARKVANRVIFMDAGKIVEDCAKEEFFGNIDARSDRAKQFLSKILHH</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LRV7</accession>
    <name>Q1LRV7_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Thiol-disulfide isomerase-like and thioredoxin-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_0233</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07119.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008652614.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0233"/>
    <dbReference type="EnsemblBacteria" id="ABF07119">
        <property type="protein sequence ID" value="ABF07119"/>
        <property type="gene ID" value="Rmet_0233"/>
    </dbReference>
    <dbReference type="GeneID" id="60822958"/>
    <dbReference type="KEGG" id="rme:Rmet_0233"/>
    <dbReference type="eggNOG" id="COG0526">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_042529_11_2_4"/>
    <dbReference type="OMA" id="RYVGEPQ"/>
    <dbReference type="OrthoDB" id="1617952at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016853">
        <property type="term" value="F:isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013740">
        <property type="entry name" value="Redoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08534">
        <property type="entry name" value="Redoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="SSF52833"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0413">Isomerase</keyword>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="16"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="domain" description="Thioredoxin" evidence="2">
        <location>
            <begin position="36"/>
            <end position="176"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF07119.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="179" mass="19456" checksum="111908BDF774B41B" modified="2006-05-30" version="1">MSNSATPATPSSKKRWPIAVAVVIVALLGFFGYRAMTPANTVPDATFTLLSGEKLSTGDLKGKVYLINFWATSCATCVKEMPQMVSTYKQFKDKGLDFVAVAMNYDPPMYVMNYAKTRELPFKVAMDADGAAAKAFGNVQLTPTTFVVDKNGKILKRYVGEPEWDALHKLLDGALANAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LS91</accession>
    <name>Q1LS91_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Dehydrogenase/reductase oxidoreductase protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_0099</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF06985.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515020.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0099"/>
    <dbReference type="EnsemblBacteria" id="ABF06985">
        <property type="protein sequence ID" value="ABF06985"/>
        <property type="gene ID" value="Rmet_0099"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0099"/>
    <dbReference type="eggNOG" id="COG4221">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010194_2_1_4"/>
    <dbReference type="OMA" id="RAMTVYA"/>
    <dbReference type="OrthoDB" id="1186478at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU000363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF06985.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="265" mass="28519" checksum="A40482BCCD7ED428" modified="2006-05-30" version="1">MRPQKIFITGASSGIGQALARAYAEQGAILGLVGRREDALRAFAGTLPNPGAVRVYAVDVRDVDAMGVAAADFLANVGCPDVVIANAGVSVGTVASEREDLEVFRTVMDTNWFGTLTTFQPFLGPMREQPPGADGWRGTLVGIASVAGVRGLPGGGAYSASKSAVIKLLESLRLEQAREKIRVVTIAPGYIRTPMTSHNPYRMPFLMDAEVFAQKAVRRIAAGSRFAVIPWQMGVVASLLHLMPRWLYDALFARAPRKPRRPRDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="96" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LS98</accession>
    <name>Q1LS98_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Signal transduction histidine kinase containing a CHASE3 sensory domain</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_0092</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF06978.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515013.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0092"/>
    <dbReference type="EnsemblBacteria" id="ABF06978">
        <property type="protein sequence ID" value="ABF06978"/>
        <property type="gene ID" value="Rmet_0092"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0092"/>
    <dbReference type="eggNOG" id="COG4585">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG5278">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_592959_0_0_4"/>
    <dbReference type="OMA" id="NHLQQVR"/>
    <dbReference type="OrthoDB" id="1755994at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007891">
        <property type="entry name" value="CHASE3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011712">
        <property type="entry name" value="Sig_transdc_His_kin_sub3_dim/P"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05227">
        <property type="entry name" value="CHASE3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07730">
        <property type="entry name" value="HisKA_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="SSF55874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Histidine kinase" evidence="2">
        <location>
            <begin position="253"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="208"/>
            <end position="235"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF06978.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="461" mass="51301" checksum="0905A9C8CE75B2D8" modified="2006-05-30" version="1">MAKHSLLVRNTLLLVSGIMLTLAVLIASETGNQRLREGYIEAIRSQQLQNDLGDLIAELVNAEGGQRGFLLTGKDSYLDPYYKALPRINELMSRIRQHYANDPEGLRQFGEASQFVTRKLNEMALTLVYGKRDFDVALDMVRTDFGKQTMENARRSLEHLQVRESGTVTHNLEAAEHDLQLSRYGIGLLTAINIVLMVTLGMGHARRMAMADATRAELEEESARLDRKVRARTRQLSALAAHLQRVTEDEKTRLARELHDELGAILTAIKLDLHWVRMRVQDTHPEARDKLTRVMQHADQAIQIKRQLIEDLRPTVLLNLGLREAIVQLVEDVGTRNQWDTALDLPETLPRLNDPAAIALYRIVQESLTNASKYAEAKQVSVTLACTTEHLVLTVRDDGRGLPPDFDAGSIAGHHGLLGMEQRVLALGGTMEVDSSPGHGVTIGIEVPLTAAVLAQEEPAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="61" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LSD4</accession>
    <name>Q1LSD4_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_0056</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF06942.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011514981.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0056"/>
    <dbReference type="EnsemblBacteria" id="ABF06942">
        <property type="protein sequence ID" value="ABF06942"/>
        <property type="gene ID" value="Rmet_0056"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0056"/>
    <dbReference type="eggNOG" id="COG3159">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_073320_1_0_4"/>
    <dbReference type="OMA" id="FQVPFVS"/>
    <dbReference type="OrthoDB" id="1264777at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007435">
        <property type="entry name" value="DUF484"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029016">
        <property type="entry name" value="GAF-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38765">
        <property type="entry name" value="PTHR38765"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04340">
        <property type="entry name" value="DUF484"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="38"/>
            <end position="65"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF06942.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="223" mass="24527" checksum="3E623051E1788106" modified="2006-05-30" version="1">MNAQDVAAYLQSHPQFFEDHAELLATVQLTSPHSHRAVSLQERQMEILREKNKGLELKLADLVRHGHENDRTQQRMHDWQMRLLAEADSHALPYAVRDGLQQVFDVPAVGLRLWSVGETYAHMEVAQGASDDLRLFAEGLRAPFCGANAGFEAAQLLERDDVASLAMVALRVPAKPDEDGPGAAFGLLVLGSPDARRFHEGMGTAYLAQIGEVAGAALNRLRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-04-26" modified="2022-08-03" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>Q58AB2</accession>
    <name>Q58AB2_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Rmet_6066</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="7 8">
        <name>pMOL30</name>
    </geneLocation>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="8">Plasmid pMOL30</plasmid>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SOS response-associated peptidase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000354">
        <property type="protein sequence ID" value="ABF12925.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011229421.1">
        <property type="nucleotide sequence ID" value="NC_007971.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_145706.1">
        <property type="nucleotide sequence ID" value="NC_006466.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF12925">
        <property type="protein sequence ID" value="ABF12925"/>
        <property type="gene ID" value="Rmet_6066"/>
    </dbReference>
    <dbReference type="GeneID" id="60825051"/>
    <dbReference type="KEGG" id="rme:Rmet_6066"/>
    <dbReference type="HOGENOM" id="CLU_035990_3_0_4"/>
    <dbReference type="OMA" id="WGIYRED"/>
    <dbReference type="OrthoDB" id="1718602at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid pMOL30"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008233">
        <property type="term" value="F:peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006974">
        <property type="term" value="P:cellular response to DNA damage stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018142">
        <property type="term" value="P:protein-DNA covalent cross-linking"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1680.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003738">
        <property type="entry name" value="SRAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036590">
        <property type="entry name" value="SRAP-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02586">
        <property type="entry name" value="SRAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143081">
        <property type="entry name" value="SSF143081"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0190">Covalent protein-DNA linkage</keyword>
    <keyword evidence="3" id="KW-0227">DNA damage</keyword>
    <keyword evidence="6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABF12925.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="228" mass="25878" checksum="BB7117663E9AC90A" modified="2005-04-26" version="1">MCVNYAPVQRQVLRDVFGVEPPSDEWRAEAWRDYPAPIIRAGDHGQRECVLAGFGMVPKAEIKRRNEETMQRTGAPPKVRDYDTMNARLETIGKLQSFAKYWRECRLALVGATAVYEPCWETGKAVRWRIGLPDGEPFAIAGLWRDWPDGSATFTMPTVAAETHALMRRMHAPGKEKRSVVILPRDEWDDWLNCRDPELARSFLRLYPADAMVAEAAPAPPRKKAVEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-04-26" modified="2022-05-25" version="117" xmlns="http://uniprot.org/uniprot">
    <accession>Q58AF7</accession>
    <name>Q58AF7_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Diguanylate cyclase/phosphodiesterase (GGDEF and EAL domains) with PAS/PAC sensor(S)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Rmet_6140</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="7 8">
        <name>pMOL30</name>
    </geneLocation>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="8">Plasmid pMOL30</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000354">
        <property type="protein sequence ID" value="ABF12999.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011229389.1">
        <property type="nucleotide sequence ID" value="NC_007971.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_145661.1">
        <property type="nucleotide sequence ID" value="NC_006466.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF12999">
        <property type="protein sequence ID" value="ABF12999"/>
        <property type="gene ID" value="Rmet_6140"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6140"/>
    <dbReference type="HOGENOM" id="CLU_000445_70_20_4"/>
    <dbReference type="OMA" id="ICFEDIS"/>
    <dbReference type="OrthoDB" id="1635706at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid pMOL30"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01948">
        <property type="entry name" value="EAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01949">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00130">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.580.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.450">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001633">
        <property type="entry name" value="EAL_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035919">
        <property type="entry name" value="EAL_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000160">
        <property type="entry name" value="GGDEF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029787">
        <property type="entry name" value="Nucleotide_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000014">
        <property type="entry name" value="PAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000700">
        <property type="entry name" value="PAS-assoc_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035965">
        <property type="entry name" value="PAS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013767">
        <property type="entry name" value="PAS_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00571">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00563">
        <property type="entry name" value="EAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00990">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00989">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00052">
        <property type="entry name" value="EAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00267">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00091">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF141868">
        <property type="entry name" value="SSF141868"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="SSF54631"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55073">
        <property type="entry name" value="SSF55073"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55785">
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        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00229">
        <property type="entry name" value="sensory_box"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="CBS"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="EAL"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="7" id="KW-0614">Plasmid</keyword>
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            <begin position="68"/>
            <end position="124"/>
        </location>
    </feature>
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        <location>
            <begin position="142"/>
            <end position="187"/>
        </location>
    </feature>
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        <location>
            <begin position="215"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="domain" description="GGDEF" evidence="5">
        <location>
            <begin position="299"/>
            <end position="432"/>
        </location>
    </feature>
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        <location>
            <begin position="441"/>
            <end position="695"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABF12999.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="705" mass="78498" checksum="59A865A5E5FBEDB6" modified="2005-04-26" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2005-04-26" modified="2022-05-25" version="97" xmlns="http://uniprot.org/uniprot">
    <accession>Q58AN8</accession>
    <name>Q58AN8_CUPMC</name>
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        <submittedName>
            <fullName evidence="3">Cytosine deaminase-like metal-dependent amidohydrolase</fullName>
        </submittedName>
    </protein>
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        <name evidence="3" type="ordered locus">Rmet_5997</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
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        <lineage>
            <taxon>Bacteria</taxon>
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    </organism>
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        <name>pMOL30</name>
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    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid pMOL30</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000354">
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_007971.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_145577.1">
        <property type="nucleotide sequence ID" value="NC_006466.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF12856">
        <property type="protein sequence ID" value="ABF12856"/>
        <property type="gene ID" value="Rmet_5997"/>
    </dbReference>
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    <dbReference type="HOGENOM" id="CLU_023620_5_1_4"/>
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    <dbReference type="OrthoDB" id="1456605at2"/>
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        <property type="component" value="Plasmid pMOL30"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016810">
        <property type="term" value="F:hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
    <dbReference type="InterPro" id="IPR011059">
        <property type="entry name" value="Metal-dep_hydrolase_composite"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01979">
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Amidohydro-rel" evidence="2">
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            <begin position="55"/>
            <end position="409"/>
        </location>
    </feature>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF01979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF12856.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="443" mass="48584" checksum="3FEA11B2D53C3467" modified="2005-04-26" version="1">MTKSVFHNVTIFDGTGSDPYAGSVLIQNDRIASIVRGDQEIDAPDARVFDGRGRFLMPGMTEAHTHFSWNDQPSLSAIQFMPPEEHILWCVRVAKRYLDMGWTSAIGAAAAKPRLDVVLRNAVNAGEFPGPRYLAGSQEITTIGGLGDNTLPHLPFEALSFGSVCSGPEEIRRSARTFIKYGVDHLKINLSGEYIAGLPAELSPFSEEEVAMLAAEAKRYGKRVAAHARSSESVKQCVRHGLELVYHASFADEEALDMLEANKDKHFVAPGIGWLVRTCFNASEYGITPEVAAKMGYKRELEIASETLSKMHKRGIRILPGGDYGFAWLPHGTNANDLQYFVDYIGMTPKEALLAATKLGGQIMMRPNELGQLREGFIADVVLIDGNPLEDLSILTDPNRIALVMKGGVIQKECRDVRPPSAALHIASEDRPLVDEGVRKAMH</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-01-04" modified="2021-09-29" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>Q5NV10</accession>
    <name>Q5NV10_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Soluble lytic murein transglycosylase, Type II secretory pathway</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">pilT</name>
        <name evidence="3" type="ordered locus">Rmet_6296</name>
    </gene>
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        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
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        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3 4">
        <name>pMOL28</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid pMOL28</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000355">
        <property type="protein sequence ID" value="ABF13155.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_007972.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_161644.1">
        <property type="nucleotide sequence ID" value="NC_006525.1"/>
    </dbReference>
    <dbReference type="CAZy" id="GH23">
        <property type="family name" value="Glycoside Hydrolase Family 23"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF13155">
        <property type="protein sequence ID" value="ABF13155"/>
        <property type="gene ID" value="Rmet_6296"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6296"/>
    <dbReference type="HOGENOM" id="CLU_094905_3_1_4"/>
    <dbReference type="OMA" id="KIYVVYT"/>
    <dbReference type="OrthoDB" id="1444566at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid pMOL28"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023346">
        <property type="entry name" value="Lysozyme-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008258">
        <property type="entry name" value="Transglycosylase_SLT_dom_1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01464">
        <property type="entry name" value="SLT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53955">
        <property type="entry name" value="SSF53955"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5013532134" evidence="1">
        <location>
            <begin position="17"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="domain" description="SLT" evidence="2">
        <location>
            <begin position="26"/>
            <end position="124"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF13155.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="140" mass="15369" checksum="6442E3C127B06359" modified="2005-01-04" version="1">MIALRIGLLIAGTAFASLVRADCLDDAAAFHRVSPILARGIAQVESGMRSWVTNRNSNGSEDIGLMQINSAHLPRLAKFGITRQSLFDPCTNAYVGTWILADCLSRYGETWTAVGCYNAGSPDKRLRYANRVYQKLQSTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-03-23" modified="2020-10-07" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>D3DXN6</accession>
    <name>D3DXN6_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_6439</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ADC45056.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_6439"/>
    <dbReference type="EnsemblBacteria" id="ADC45056">
        <property type="protein sequence ID" value="ADC45056"/>
        <property type="gene ID" value="Rmet_6439"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6439"/>
    <dbReference type="HOGENOM" id="CLU_3102964_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADC45056.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="51" mass="5448" checksum="C1929075A3C7104C" modified="2010-03-23" version="1">MRQGVAQAAGASDADRWLQVNPLRARMFADLSEVLRAYVVDKAPADEGGAC</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-03-23" modified="2020-10-07" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>D3DXP4</accession>
    <name>D3DXP4_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_6449</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ADC45064.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_6449"/>
    <dbReference type="EnsemblBacteria" id="ADC45064">
        <property type="protein sequence ID" value="ADC45064"/>
        <property type="gene ID" value="Rmet_6449"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6449"/>
    <dbReference type="HOGENOM" id="CLU_3256803_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADC45064.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="42" mass="4285" checksum="B09740953C4E5553" modified="2010-03-23" version="1">MGDSVSQPGWVRRAPDVPYARLSAKIAILANAPAPAQPAGAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-03-23" modified="2020-10-07" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>D3DXR2</accession>
    <name>D3DXR2_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_6472</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ADC45082.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADC45082">
        <property type="protein sequence ID" value="ADC45082"/>
        <property type="gene ID" value="Rmet_6472"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6472"/>
    <dbReference type="HOGENOM" id="CLU_3398082_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADC45082.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="31" mass="3643" checksum="B54FF9ACF15C255B" modified="2010-03-23" version="1">MSKLTRAFRESNRNFAVQKKKKLALLRALSH</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-03-23" modified="2020-10-07" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>D3DXS0</accession>
    <name>D3DXS0_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_6482</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ADC45090.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_6482"/>
    <dbReference type="EnsemblBacteria" id="ADC45090">
        <property type="protein sequence ID" value="ADC45090"/>
        <property type="gene ID" value="Rmet_6482"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6482"/>
    <dbReference type="HOGENOM" id="CLU_2525141_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADC45090.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="84" mass="8724" checksum="D9BE2BE48A01C81D" modified="2010-03-23" version="1">MLTPAARASGAAGEGLATGSALAGLSETGLRAMKSSLLGCDFGSLGARIWAIYATVYRQFEEKSAAITCCRAVAPRTRRFAEPI</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-03-23" modified="2021-06-02" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>D3DXX2</accession>
    <name>D3DXX2_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_6535</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ADC45142.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517131.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_6535"/>
    <dbReference type="EnsemblBacteria" id="ADC45142">
        <property type="protein sequence ID" value="ADC45142"/>
        <property type="gene ID" value="Rmet_6535"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6535"/>
    <dbReference type="HOGENOM" id="CLU_2846689_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADC45142.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="65" mass="7375" checksum="B99A453931C2DF9B" modified="2010-03-23" version="1">MQHAKGSTRKQRKRMLEGDDPHDDLRMRLELFIEKPSDHGRLLSLIGGLVDLELSARLSEVLPPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-03-23" modified="2020-10-07" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>D3DY51</accession>
    <name>D3DY51_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_6622</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ADC45221.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_6622"/>
    <dbReference type="EnsemblBacteria" id="ADC45221">
        <property type="protein sequence ID" value="ADC45221"/>
        <property type="gene ID" value="Rmet_6622"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6622"/>
    <dbReference type="HOGENOM" id="CLU_2846693_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADC45221.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="65" mass="7533" checksum="ED9C1A6635C92F27" modified="2010-03-23" version="1">MVPCWPNYPAIPKRGIEYRVMGRYAIWTGIIPKVGWVKEGQVLSFASRTRLATRGSNYYLSKNRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="50" xmlns="http://uniprot.org/uniprot">
    <accession>Q1L9Q1</accession>
    <name>Q1L9Q1_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_6266</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="1 2">
        <name>pMOL28</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid pMOL28</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000355">
        <property type="protein sequence ID" value="ABF13125.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011514902.1">
        <property type="nucleotide sequence ID" value="NC_007972.2"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF13125">
        <property type="protein sequence ID" value="ABF13125"/>
        <property type="gene ID" value="Rmet_6266"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6266"/>
    <dbReference type="HOGENOM" id="CLU_2587203_0_0_4"/>
    <dbReference type="OMA" id="LNEFWQI"/>
    <dbReference type="OrthoDB" id="2089299at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid pMOL28"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF13125.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="81" mass="8913" checksum="D7E25658F02F7410" modified="2006-05-30" version="1">MTQHDDNEREYPEPETVLAIRGAIATGQLGGPKGPPGHWLNEFWQIGAALRDHAEILQGFEDTALQELLNTTADYLATDVP</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="54" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LA26</accession>
    <name>Q1LA26_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_6141</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="1 2">
        <name>pMOL30</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid pMOL30</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000354">
        <property type="protein sequence ID" value="ABF13000.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008642702.1">
        <property type="nucleotide sequence ID" value="NC_007971.2"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF13000">
        <property type="protein sequence ID" value="ABF13000"/>
        <property type="gene ID" value="Rmet_6141"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6141"/>
    <dbReference type="HOGENOM" id="CLU_167402_0_0_4"/>
    <dbReference type="OMA" id="FEIPPMQ"/>
    <dbReference type="OrthoDB" id="1900669at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid pMOL30"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF13000.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="111" mass="12384" checksum="C2F91E928AB1AD5D" modified="2006-05-30" version="1">MEKRRQKRLRAHIKEFVLPPITDGLVLGRASPIGHVAVGRALSLLSTTAFEHIEIDDDVISDILIRKAILRKIPAAELKTFILDEIKPAMGAEEILHLALEVELFVDQKLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-10-07" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LA90</accession>
    <name>Q1LA90_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_6077</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="1 2">
        <name>pMOL30</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid pMOL30</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000354">
        <property type="protein sequence ID" value="ABF12936.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF12936">
        <property type="protein sequence ID" value="ABF12936"/>
        <property type="gene ID" value="Rmet_6077"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6077"/>
    <dbReference type="HOGENOM" id="CLU_1298908_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid pMOL30"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF12936.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="212" mass="23012" checksum="94C098D4B81BA9F0" modified="2006-05-30" version="1">MGKKRSFPMLLLFPSALTQNSAGEHKKAAREQFSGIKSSHLSEAIASGFGYQSHAALLAALGSNARPRSNFSLIGFGKRLTELGYERPADFLADQDVPWAVQCLVSLMIEAGFRDSLASNSSRIGVTKTIDALNVAGCTEFNFKALGRQAQQQQTRALRELFSTVIELQVSVRDGDRVLRSPIVREVGIETPTNCILFSLADVIVRAARLAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-10-07" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LAA6</accession>
    <name>Q1LAA6_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_6061</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="1 2">
        <name>pMOL30</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid pMOL30</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000354">
        <property type="protein sequence ID" value="ABF12920.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF12920">
        <property type="protein sequence ID" value="ABF12920"/>
        <property type="gene ID" value="Rmet_6061"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6061"/>
    <dbReference type="HOGENOM" id="CLU_1365271_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid pMOL30"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF12920.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="200" mass="22410" checksum="F18E3973F11E9DDE" modified="2006-05-30" version="1">MMAPSTSQKHHRPWCERSFFTVKSQWSLNRRLRSGSNFKPAVSIQGRGRTSLRQAGLRRNRSARCIPSQGALGERPVSAHCAERTTHCVLCASSRFHRETSGITIFTVKTNAIAGRALGIPYKYCYVLCIAIVRNEELVAVRKWQESTLGTSPSTSHSVKRDHREIGYRLAEVALSRTPRMNTGNFEKEAGGFVPRVGKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LAN1</accession>
    <name>Q1LAN1_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative monooxygenase with luciferase-like ATPase activity</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_5936</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">To bacterial alkanal monooxygenase alpha and beta chains.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12795.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5936"/>
    <dbReference type="EnsemblBacteria" id="ABF12795">
        <property type="protein sequence ID" value="ABF12795"/>
        <property type="gene ID" value="Rmet_5936"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5936"/>
    <dbReference type="eggNOG" id="COG2141">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027853_9_1_4"/>
    <dbReference type="OMA" id="APGSDQM"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011251">
        <property type="entry name" value="Luciferase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036661">
        <property type="entry name" value="Luciferase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019949">
        <property type="entry name" value="Luciferase_OxRdatse_group-1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00296">
        <property type="entry name" value="Bac_luciferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51679">
        <property type="entry name" value="SSF51679"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03558">
        <property type="entry name" value="oxido_grp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Bac_luciferase" evidence="2">
        <location>
            <begin position="22"/>
            <end position="302"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007789"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF12795.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="343" mass="37403" checksum="CC9C2F6C2D06411E" modified="2006-05-30" version="1">MQAFPMTPYSLLDLSPIIEGSDATQAMRNTLSLAQHAERLGYRRFWLAEHHNMPGIASAATAVLIGYVANGTSTIRVGSGGIMLPNHSPLVVAEQFGTLASLYPGRIDLGLGRAPGTDQATARALRRTLSHDSADSFPQDVEELQAYFDEVRPGQRVRAVPGAGLKVPLWLLGSSLFGAQLAAAMGLPFAFASHFAPGFMRQAVDLYRRTFRPSEQLDRPHVMLGLNVFAAETDAEARRLFSSLQQQFLALVRGTPGQLKPPVDDIERLWDASEAEHIRRSLACSVVGDPATVREGIQRFVDDLRPDELMLTGQIYDHAARLRSFEIAADAMRALQPASSQTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="65" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LAP4</accession>
    <name>Q1LAP4_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_5923</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12782.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518132.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5923"/>
    <dbReference type="EnsemblBacteria" id="ABF12782">
        <property type="protein sequence ID" value="ABF12782"/>
        <property type="gene ID" value="Rmet_5923"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5923"/>
    <dbReference type="eggNOG" id="ENOG50324B7">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_119404_0_0_4"/>
    <dbReference type="OMA" id="NIWTGEY"/>
    <dbReference type="OrthoDB" id="1771777at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010835">
        <property type="entry name" value="DUF1439"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07273">
        <property type="entry name" value="DUF1439"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193247" evidence="1">
        <location>
            <begin position="32"/>
            <end position="193"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF12782.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="193" mass="21169" checksum="2E8C8E1A5F94728A" modified="2006-05-30" version="1">MSRTLINRLSHARRPLLALAIVAAALGTAHAGYNVWTGEYTFTKDELQSAVDKRFPATLRYGELVSVRLSNPRLVLDEAGNRITTRVDAALSNTVIPSPPVNGSLALNSGVRYDATRRAVLLDNPTVQDVEVQGMSQYREQLNAIGAVVAQQLLKDYPLYTFKPEELRFNGKDVEPGAITVAPDGIRVQVNLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="64" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LAX9</accession>
    <name>Q1LAX9_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Putative Seryl-tRNA synthetase SerS2-like protein</fullName>
            <ecNumber evidence="1">6.1.1.11</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_5838</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EC" id="6.1.1.11" evidence="1"/>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12697.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518047.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5838"/>
    <dbReference type="EnsemblBacteria" id="ABF12697">
        <property type="protein sequence ID" value="ABF12697"/>
        <property type="gene ID" value="Rmet_5838"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5838"/>
    <dbReference type="eggNOG" id="COG0172">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_054340_0_0_4"/>
    <dbReference type="OMA" id="RFPPVMS"/>
    <dbReference type="OrthoDB" id="596492at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004828">
        <property type="term" value="F:serine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="SSF55681"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="1" id="KW-0436">Ligase</keyword>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF12697.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="321" mass="35293" checksum="09D84D5EA565C9BD" modified="2006-05-30" version="1">MDAHTLTEARPAGGADPMAAQPPTFLESLLAAGLLIQTGVRGLYGRSAVFEGIADGLNAAFTRIGADQNAEVLRFPPAIGKDDFETSEYLKSFPQLAGTIHSFCGDDRGHRQLLARLEDKQDWTDQQQFTGVVMTPAACYPIYPVIGKRGPLPAEGKIVDVMSYCYRHEPSLEPTRMQLFRQREYVCLGSPERIVAFRELWMERGLAFGRALQLPVEVDVANDPFFGRGGKIVADSQRELKLKFELLIPVNDGAPPTACMSFNYHMDHFGALWHIGLHDGGVAHTGCVGFGIERLVLALLKHHGLDPARWPQAVRETLGGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-10-07" version="63" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LBD4</accession>
    <name>Q1LBD4_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_5683</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12542.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF12542">
        <property type="protein sequence ID" value="ABF12542"/>
        <property type="gene ID" value="Rmet_5683"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5683"/>
    <dbReference type="eggNOG" id="ENOG50336B2">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1685142_0_0_4"/>
    <dbReference type="OMA" id="ITPRRAW"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="92"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="124"/>
            <end position="149"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF12542.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="149" mass="16054" checksum="8C45674A4ACA58A7" modified="2006-05-30" version="1">MTRATASIPLRGALLLIIVLAQLLTPLLHGHIGTPNQTGLHVHTSPSRSFDSHSRFEGLSGHGDHHHHAFGSHREPFEVDVETAIGPFSFSLLLQAAAVLGASVLTFVLLAALAVYITPRRAWRPASPRGRWSSRVGWPPPAQAPPTFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="97" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LBI6</accession>
    <name>Q1LBI6_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Transcriptional regulator, GntR family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Rmet_5631</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="6">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="6">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12490.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011520034.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5631"/>
    <dbReference type="EnsemblBacteria" id="ABF12490">
        <property type="protein sequence ID" value="ABF12490"/>
        <property type="gene ID" value="Rmet_5631"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5631"/>
    <dbReference type="eggNOG" id="COG1802">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017584_5_1_4"/>
    <dbReference type="OMA" id="DNHELYI"/>
    <dbReference type="OrthoDB" id="1911275at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07377">
        <property type="entry name" value="WHTH_GntR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.530">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011711">
        <property type="entry name" value="GntR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008920">
        <property type="entry name" value="TF_FadR/GntR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000524">
        <property type="entry name" value="Tscrpt_reg_HTH_GntR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07729">
        <property type="entry name" value="FCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00392">
        <property type="entry name" value="GntR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00895">
        <property type="entry name" value="FCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00345">
        <property type="entry name" value="HTH_GNTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48008">
        <property type="entry name" value="SSF48008"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50949">
        <property type="entry name" value="HTH_GNTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0614">Plasmid</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH gntR-type" evidence="4">
        <location>
            <begin position="15"/>
            <end position="82"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABF12490.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="229" mass="25918" checksum="61B1F10EC71214CC" modified="2006-05-30" version="1">MSTNPTTPSQAVDRQILHVAVAQRLRTMIMEGDLAPGTRLNERVLCDLLQVSRTPLREAFKVLAGDGLVTLLPNRGAEVVVLSQEAINHLFEMMGALEAMSGELACQRITDAELNEIRALHYEMLACHARRDLPNYYALNRRIHDAINAAARNPILEETYRRINLRIQALRFRSNFDQDKWDMAVREHGEMLDALSKRDGAALRDILRAHLQHKHDALIAELERAQDAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="67" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LBQ4</accession>
    <name>Q1LBQ4_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">AAA_13 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_5563</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12422.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5563"/>
    <dbReference type="EnsemblBacteria" id="ABF12422">
        <property type="protein sequence ID" value="ABF12422"/>
        <property type="gene ID" value="Rmet_5563"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5563"/>
    <dbReference type="eggNOG" id="COG4694">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020729_2_0_4"/>
    <dbReference type="OMA" id="LTHNVYF"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026866">
        <property type="entry name" value="CR006_AAA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13166">
        <property type="entry name" value="AAA_13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA_13" evidence="1">
        <location>
            <begin position="30"/>
            <end position="774"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF12422.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="792" mass="89244" checksum="0D6368950A11371A" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="92" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LBR0</accession>
    <name>Q1LBR0_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Enoyl-CoA hydratase/isomerase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Rmet_5557</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="5">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="5">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the enoyl-CoA hydratase/isomerase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12416.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519963.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5557"/>
    <dbReference type="EnsemblBacteria" id="ABF12416">
        <property type="protein sequence ID" value="ABF12416"/>
        <property type="gene ID" value="Rmet_5557"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5557"/>
    <dbReference type="eggNOG" id="COG1024">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009834_7_6_4"/>
    <dbReference type="OMA" id="ESARFCT"/>
    <dbReference type="OrthoDB" id="1275789at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016853">
        <property type="term" value="F:isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.12.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029045">
        <property type="entry name" value="ClpP/crotonase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018376">
        <property type="entry name" value="Enoyl-CoA_hyd/isom_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001753">
        <property type="entry name" value="Enoyl-CoA_hydra/iso"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014748">
        <property type="entry name" value="Enoyl-CoA_hydra_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00378">
        <property type="entry name" value="ECH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52096">
        <property type="entry name" value="SSF52096"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00166">
        <property type="entry name" value="ENOYL_COA_HYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0413">Isomerase</keyword>
    <keyword evidence="4" id="KW-0614">Plasmid</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF12416.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="264" mass="28283" checksum="208342780384134E" modified="2006-05-30" version="1">MSLRYGSVLYAVKGSVAIVTLNRPEFRNALGGTIREDIIEVMAVAEANDSVRAIILTGAGSAFCSGGDLNELYLRAVQGQTIAEKTEPIRDRTLLAVYEAKKPVIAAVNGPAMGAGMNLALAADIRIASKEARFSQAHTMRGMMPDYGGTYLLPALLGSSKAYELICTGATLDAEEALRLGLVSDVVEPSTLMDRARTMAQAIALNAPIPIRLAKRAVQQHNLGGLREALARETAAQNVCYESQDAREGLRSFLEKRTPTFQGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LC09</accession>
    <name>Q1LC09_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Tn7-like transposase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">tnsB</name>
        <name evidence="3" type="ordered locus">Rmet_5458</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12317.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519860.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5458"/>
    <dbReference type="EnsemblBacteria" id="ABF12317">
        <property type="protein sequence ID" value="ABF12317"/>
        <property type="gene ID" value="Rmet_5458"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5458"/>
    <dbReference type="eggNOG" id="COG2801">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019011_1_1_4"/>
    <dbReference type="OMA" id="PSWNGAR"/>
    <dbReference type="OrthoDB" id="1637053at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001584">
        <property type="entry name" value="Integrase_cat-core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015378">
        <property type="entry name" value="Transposase-like_Mu_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09299">
        <property type="entry name" value="Mu-transpos_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="SSF53098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50994">
        <property type="entry name" value="INTEGRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Integrase catalytic" evidence="2">
        <location>
            <begin position="272"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="674"/>
            <end position="693"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF12317.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="725" mass="81953" checksum="B6909B22E598E0C5" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="107" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LC71</accession>
    <name>Q1LC71_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Cation-transporting ATPase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">Rmet_5396</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="10">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="10">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12255.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519801.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5396"/>
    <dbReference type="EnsemblBacteria" id="ABF12255">
        <property type="protein sequence ID" value="ABF12255"/>
        <property type="gene ID" value="Rmet_5396"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5396"/>
    <dbReference type="eggNOG" id="COG0474">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002360_3_3_4"/>
    <dbReference type="OMA" id="GVHRMAK"/>
    <dbReference type="OrthoDB" id="178322at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006811">
        <property type="term" value="P:ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1110.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006068">
        <property type="entry name" value="ATPase_P-typ_cation-transptr_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004014">
        <property type="entry name" value="ATPase_P-typ_cation-transptr_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023299">
        <property type="entry name" value="ATPase_P-typ_cyto_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018303">
        <property type="entry name" value="ATPase_P-typ_P_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023298">
        <property type="entry name" value="ATPase_P-typ_TM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008250">
        <property type="entry name" value="ATPase_P-typ_transduc_dom_A_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001757">
        <property type="entry name" value="P_typ_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044492">
        <property type="entry name" value="P_typ_ATPase_HD_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00689">
        <property type="entry name" value="Cation_ATPase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00690">
        <property type="entry name" value="Cation_ATPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00120">
        <property type="entry name" value="HATPASE"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00027">
        <property type="entry name" value="p-type_atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00831">
        <property type="entry name" value="Cation_ATPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="SSF56784"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81653">
        <property type="entry name" value="SSF81653"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81660">
        <property type="entry name" value="SSF81660"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81665">
        <property type="entry name" value="SSF81665"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01494">
        <property type="entry name" value="ATPase_P-type"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00154">
        <property type="entry name" value="ATPASE_E1_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-0614">Plasmid</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-1278">Translocase</keyword>
    <keyword evidence="1 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="61"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="89"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="253"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="286"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="701"/>
            <end position="725"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="731"/>
            <end position="749"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="769"/>
            <end position="792"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="804"/>
            <end position="822"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="842"/>
            <end position="861"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="876"/>
            <end position="893"/>
        </location>
    </feature>
    <feature type="domain" description="Cation_ATPase_N" evidence="8">
        <location>
            <begin position="15"/>
            <end position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ABF12255.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="920" mass="98744" checksum="A281CE3F968A7D78" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="101" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LC94</accession>
    <name>Q1LC94_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="10">TonB-dependent outer-membrane transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">Rmet_5373</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
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        <name>megaplasmid CH34</name>
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        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="11">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cell outer membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the TonB-dependent receptor family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12232.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519779.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5373"/>
    <dbReference type="EnsemblBacteria" id="ABF12232">
        <property type="protein sequence ID" value="ABF12232"/>
        <property type="gene ID" value="Rmet_5373"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5373"/>
    <dbReference type="eggNOG" id="COG4771">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008287_18_0_4"/>
    <dbReference type="OMA" id="PDKEKMM"/>
    <dbReference type="OrthoDB" id="71288at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009279">
        <property type="term" value="C:cell outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.130.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.170.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039426">
        <property type="entry name" value="BtuB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012910">
        <property type="entry name" value="Plug_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037066">
        <property type="entry name" value="Plug_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000531">
        <property type="entry name" value="TonB-dep_rcpt_b-brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036942">
        <property type="entry name" value="TonB_rcpt_b-brl_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30069">
        <property type="entry name" value="PTHR30069"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07715">
        <property type="entry name" value="Plug"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00593">
        <property type="entry name" value="TonB_dep_Rec"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0998">Cell outer membrane</keyword>
    <keyword evidence="3 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-0675">Receptor</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="2 6" id="KW-0798">TonB box</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193321" evidence="7">
        <location>
            <begin position="22"/>
            <end position="661"/>
        </location>
    </feature>
    <feature type="domain" description="Plug" evidence="9">
        <location>
            <begin position="46"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="domain" description="TonB_dep_Rec" evidence="8">
        <location>
            <begin position="249"/>
            <end position="656"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009810"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003357"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00593"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF07715"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ABF12232.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="661" mass="73252" checksum="C93905E5B1C680D6" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LCC7</accession>
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        <submittedName>
            <fullName evidence="6">Hemolysin activator translocator</fullName>
        </submittedName>
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        <name evidence="6" type="primary">hlyB</name>
        <name evidence="6" type="ordered locus">Rmet_5340</name>
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        <lineage>
            <taxon>Bacteria</taxon>
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        <name>megaplasmid CH34</name>
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    <reference evidence="7" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
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                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
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                <person name="Vallaeys T."/>
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                <person name="Monchy S."/>
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                <person name="McCorkle S."/>
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            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="7">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
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        <property type="protein sequence ID" value="ABF12199.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5340"/>
    <dbReference type="EnsemblBacteria" id="ABF12199">
        <property type="protein sequence ID" value="ABF12199"/>
        <property type="gene ID" value="Rmet_5340"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5340"/>
    <dbReference type="eggNOG" id="COG2831">
        <property type="taxonomic scope" value="Bacteria"/>
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    <dbReference type="HOGENOM" id="CLU_020581_2_0_4"/>
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    <dbReference type="OrthoDB" id="956274at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005565">
        <property type="entry name" value="Hemolysn_activator_HlyB_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013686">
        <property type="entry name" value="Polypept-transport_assoc_ShlB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035251">
        <property type="entry name" value="ShlB_POTRA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027282">
        <property type="entry name" value="TPS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08479">
        <property type="entry name" value="POTRA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
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    <dbReference type="Pfam" id="PF03865">
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <location>
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            <end position="27"/>
        </location>
    </feature>
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            <end position="588"/>
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        <location>
            <begin position="101"/>
            <end position="175"/>
        </location>
    </feature>
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        <location>
            <begin position="177"/>
            <end position="229"/>
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    </feature>
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        <location>
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            <end position="548"/>
        </location>
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            <end position="58"/>
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        <location>
            <begin position="28"/>
            <end position="53"/>
        </location>
    </feature>
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        <source>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF17287"/>
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    </evidence>
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        <source>
            <dbReference type="EMBL" id="ABF12199.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="588" mass="64249" checksum="9F64EA903D61B862" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="115" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LCE1</accession>
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        <submittedName>
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        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
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                <person name="Monsieurs P."/>
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            </authorList>
            <dbReference type="PubMed" id="20463976"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
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    <dbReference type="KEGG" id="rme:Rmet_5326"/>
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    <dbReference type="OrthoDB" id="1020672at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
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        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
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        <property type="project" value="InterPro"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="CheY-like_superfamily"/>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
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        <location>
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        </location>
    </feature>
    <feature type="domain" description="OmpR/PhoB-type" evidence="5">
        <location>
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            <end position="219"/>
        </location>
    </feature>
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        <location>
            <begin position="124"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="2">
        <location>
            <position position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABF12185.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="221" mass="24160" checksum="DC4B60AE6CA0F3D1" modified="2006-05-30" version="1">MRVLLVEDDRMIGQVVQDALKDASCAADWVEDGQAALDALDRQHYDVVLLDLGLPRKSGHAVLTSLRARGSAVPVLILTARDALEDRIGGLDAGADDYLVKPFEMDELLARMRAVWRRRSGLASSVLGNGRVSLDLSTRQATLTGGAPQQLTNREFALLQALLARPGAILSRAELEERIYGWGDEVESNAVEFLIHGLRRKLGSTIIRNVRGVGWQVPKEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="59" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LCE2</accession>
    <name>Q1LCE2_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Permease of the Major facilitator protein family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">zneP</name>
        <name evidence="2" type="ordered locus">Rmet_5325</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12184.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519727.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5325"/>
    <dbReference type="EnsemblBacteria" id="ABF12184">
        <property type="protein sequence ID" value="ABF12184"/>
        <property type="gene ID" value="Rmet_5325"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5325"/>
    <dbReference type="eggNOG" id="COG4705">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_070268_0_0_4"/>
    <dbReference type="OMA" id="DWMADSS"/>
    <dbReference type="OrthoDB" id="1466669at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007136">
        <property type="entry name" value="DUF347"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03988">
        <property type="entry name" value="DUF347"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="44"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="73"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="99"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="138"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="164"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="193"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="225"/>
            <end position="244"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF12184.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="254" mass="26898" checksum="9992FEC8383A149B" modified="2006-05-30" version="1">MNTSTTHHALSKVPEITLGFWLIKIAATTLGETGGDALSMSLNLGYLVSTAIFAVVFLAAVSAQIRARAFRPALYWLTIVATTTVGTTLADFADRSLGIGYAGGSALLLALLLLSLAAWYRSMGTVSVDTVNSPKAELFYWVTIMFSQTLGTALGDWTADTIGLGYLGGMLVFVTLLAALAAAYYWTSISRTLLFWAAFILTRPLGAVVGDFLDKPLQSGGLALSRFSASAALVSFMVICILLLPQRAAARASH</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LCL4</accession>
    <name>Q1LCL4_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Flagellar protein FlaG</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">flaG</name>
        <name evidence="2" type="ordered locus">Rmet_5253</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12112.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519658.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5253"/>
    <dbReference type="EnsemblBacteria" id="ABF12112">
        <property type="protein sequence ID" value="ABF12112"/>
        <property type="gene ID" value="Rmet_5253"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5253"/>
    <dbReference type="eggNOG" id="COG1334">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_120910_4_4_4"/>
    <dbReference type="OMA" id="VRQIPNA"/>
    <dbReference type="OrthoDB" id="1913585at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005186">
        <property type="entry name" value="FlaG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035924">
        <property type="entry name" value="FlaG-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37166">
        <property type="entry name" value="PTHR37166"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03646">
        <property type="entry name" value="FlaG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160214">
        <property type="entry name" value="SSF160214"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0966">Cell projection</keyword>
    <keyword evidence="2" id="KW-0969">Cilium</keyword>
    <keyword evidence="2" id="KW-0282">Flagellum</keyword>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="22"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="28"/>
            <end position="43"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF12112.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="126" mass="12999" checksum="63A1BC903F51A73A" modified="2006-05-30" version="1">MATQTAPSSGVVQAAVGVSLPQPGLRASATPADTSQAPAGVTSATPAPDQPAVRPSTDAAVRKLTEALRAASVSVQFEIDRETNRVVTKIVDKANGEIIRQIPTEEVVRIADALAQLQGLFVNQTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LCM5</accession>
    <name>Q1LCM5_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_5242</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12101.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519647.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5242"/>
    <dbReference type="EnsemblBacteria" id="ABF12101">
        <property type="protein sequence ID" value="ABF12101"/>
        <property type="gene ID" value="Rmet_5242"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5242"/>
    <dbReference type="eggNOG" id="COG4122">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_070011_0_0_4"/>
    <dbReference type="OMA" id="NCAPPEV"/>
    <dbReference type="OrthoDB" id="1948290at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008884">
        <property type="entry name" value="TylF_MeTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40036">
        <property type="entry name" value="PTHR40036"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05711">
        <property type="entry name" value="TylF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF12101.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="254" mass="28509" checksum="B4EA016D8BCCFEA8" modified="2006-05-30" version="1">MEKTSPDFLAVLRTERRDLLNRMRQTRFESLREGCSHSQVIPFATFSPWLDDEAFSAIYEIVKPFTMVDIYRCYELYSLARQCRRVAGEIVEVGVWRGGTGALMAHALNNRPVHLFDTFSGVVKHDAEFDTLYSGGEHADTDEDIVRSLFAKANVECRIHRGIFPDDTLQGLPASICLAHIDVDTYRSVKESFAEIWPRVQPGGMVVFDDFAVFGCEGANQAVNEIVAAHDDGLFIHNLNGHALLVKQPAMARS</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LCS7</accession>
    <name>Q1LCS7_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Outer membrane porin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Rmet_5190</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="8">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="8">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12049.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519596.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5190"/>
    <dbReference type="EnsemblBacteria" id="ABF12049">
        <property type="protein sequence ID" value="ABF12049"/>
        <property type="gene ID" value="Rmet_5190"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5190"/>
    <dbReference type="eggNOG" id="COG3203">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038238_1_1_4"/>
    <dbReference type="OMA" id="IGANYAN"/>
    <dbReference type="OrthoDB" id="1064531at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046930">
        <property type="term" value="C:pore complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015288">
        <property type="term" value="F:porin activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006811">
        <property type="term" value="P:ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00342">
        <property type="entry name" value="gram_neg_porins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.160.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033900">
        <property type="entry name" value="Gram_neg_porin_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023614">
        <property type="entry name" value="Porin_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002299">
        <property type="entry name" value="Porin_Neis"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13609">
        <property type="entry name" value="Porin_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00184">
        <property type="entry name" value="NEISSPPORIN"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0406">Ion transport</keyword>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-0626">Porin</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1134">Transmembrane beta strand</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193008" evidence="5">
        <location>
            <begin position="29"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="domain" description="Porin_4" evidence="6">
        <location>
            <begin position="19"/>
            <end position="336"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF13609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABF12049.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="371" mass="39804" checksum="11B6E4D82BAA2681" modified="2006-05-30" version="1">MEPLYRGRLAPFVASFPVLLCAATPAAAQSDGVQIYGFIKENVESIQISGNGRSQSLARLSNDLSVLGFRGGESLGGGNRAFFQIETNLRMDGTGTAQIGDRNTAVGLSGPWGQFWMGQWEMPLRYVSVYKVDPFTAGIFASNSIMGNGFVTAANGVSPQSFDRRQPNLVQYATPDWGGVSARVAVSMPENRTATTNPIAWSGLVSYESGQWYAAWGHEYHGDYFYSGSHDYADRVGLVYAIGGVRLRAAYERLSYEPAPGQSVRRDAWQAAATYNRGPHTFRASYTQGGNAHGNTVQAVGGIGLPGTDSGAWQVSLGYGYTLSKRTELWAAYTRLVNHATASYNLSANSVPGLKAGDAVSGFGLGITHNF</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LCU6</accession>
    <name>Q1LCU6_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Cold-shock DNA-binding domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">cspA</name>
        <name evidence="3" type="ordered locus">Rmet_5171</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12030.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5171"/>
    <dbReference type="EnsemblBacteria" id="ABF12030">
        <property type="protein sequence ID" value="ABF12030"/>
        <property type="gene ID" value="Rmet_5171"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5171"/>
    <dbReference type="eggNOG" id="COG1278">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1979708_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04458">
        <property type="entry name" value="CSP_CDS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011129">
        <property type="entry name" value="CSD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019844">
        <property type="entry name" value="CSD_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002059">
        <property type="entry name" value="CSP_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00313">
        <property type="entry name" value="CSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00050">
        <property type="entry name" value="COLDSHOCK"/>
    </dbReference>
    <dbReference type="SMART" id="SM00357">
        <property type="entry name" value="CSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00352">
        <property type="entry name" value="CSD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51857">
        <property type="entry name" value="CSD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CSD" evidence="2">
        <location>
            <begin position="60"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU000408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF12030.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="126" mass="14162" checksum="5A4C3A68FDA457FE" modified="2006-05-30" version="1">MFIDRGAVYLYKYDRRIFKRNLEALGVYLGSTTFSPCFLLDRHASSAISARREFLRNFYMETGTVKWFNDSKGFGFITPDAGGDDLFAHFSEVQGNGFKSLQEGQKVRYVAGVGQKGPAATKIEPI</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="104" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LCV2</accession>
    <name>Q1LCV2_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Two component transcriptional regulator, LuxR family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Rmet_5165</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="7">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="7">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12024.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519569.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5165"/>
    <dbReference type="EnsemblBacteria" id="ABF12024">
        <property type="protein sequence ID" value="ABF12024"/>
        <property type="gene ID" value="Rmet_5165"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5165"/>
    <dbReference type="eggNOG" id="COG2197">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_90_10_4"/>
    <dbReference type="OMA" id="DDPMMQL"/>
    <dbReference type="OrthoDB" id="930905at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06170">
        <property type="entry name" value="LuxR_C_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000792">
        <property type="entry name" value="Tscrpt_reg_LuxR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00196">
        <property type="entry name" value="GerE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00038">
        <property type="entry name" value="HTHLUXR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00421">
        <property type="entry name" value="HTH_LUXR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00622">
        <property type="entry name" value="HTH_LUXR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50043">
        <property type="entry name" value="HTH_LUXR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="6" id="KW-0614">Plasmid</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Response regulatory" evidence="5">
        <location>
            <begin position="15"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="domain" description="HTH luxR-type" evidence="4">
        <location>
            <begin position="163"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="152"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="2">
        <location>
            <position position="67"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABF12024.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="231" mass="24815" checksum="5382994E9D42F31F" modified="2006-05-30" version="1">MPLRTLAADLTLPAPALLVEDDPLVSRRLENLLQQLGYAPDALVLAASLAEARARVTDQPVALALVDLGLPDGSGIDLIAELRAADPGLGILVISAWSTQDAILAALRAGATGYVVKERDDLEVALALRSVLRGGAPIDPFIARRIIEELHPGPSESANAQTPEPSTERLSPREHQILRLVADGLGNREIAERLFLSRYTVECHIKHIYRKLAVSSRTRAINAARSRGLLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LD42</accession>
    <name>Q1LD42_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">5-methyltetrahydropteroyltriglutamate-homocysteine methyltransferase</fullName>
            <ecNumber evidence="2">2.1.1.14</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">metE</name>
        <name evidence="2" type="ordered locus">Rmet_5073</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EC" id="2.1.1.14" evidence="2"/>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11934.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519482.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5073"/>
    <dbReference type="EnsemblBacteria" id="ABF11934">
        <property type="protein sequence ID" value="ABF11934"/>
        <property type="gene ID" value="Rmet_5073"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5073"/>
    <dbReference type="eggNOG" id="COG0620">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_040013_3_0_4"/>
    <dbReference type="OMA" id="DPDCGMR"/>
    <dbReference type="OrthoDB" id="577156at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003871">
        <property type="term" value="F:5-methyltetrahydropteroyltriglutamate-homocysteine S-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009086">
        <property type="term" value="P:methionine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03311">
        <property type="entry name" value="CIMS_C_terminal_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.210">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002629">
        <property type="entry name" value="Met_Synth_C/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016456">
        <property type="entry name" value="Met_Synthase_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038071">
        <property type="entry name" value="UROD/MetE-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01717">
        <property type="entry name" value="Meth_synt_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005632">
        <property type="entry name" value="Met_synth_catalytic_prd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51726">
        <property type="entry name" value="SSF51726"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Meth_synt_2" evidence="1">
        <location>
            <begin position="5"/>
            <end position="329"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01717"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF11934.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="342" mass="38530" checksum="940EB9CD2098A132" modified="2006-05-30" version="1">MKKLLPTSTAGSLPKPSWLAQPEKLWSPWKLQDDELIEGKQDALRLSLQEQQQAGIDIVSDGEQTRQHFVTTFIENLSGVDFEKRETVRIRDRYDASVPTVVGAVSRQKPVFVEDAKFLRQQTRQPIKWALPGPMTMIDTLYDNHYKSREKLAWEFATILNQEARELEAAGVDIIQFDEPAFNVFFDEVNDWGVATLERAIEGLKCETAVHICYGYGIKANTDWKKTLGSEWRQYEESFPKLQKSSIDIVSLECHNSYVPIDLIELIRGKKVMVGAIDVATNTIETPEEVANTLRKALRFVDADKLYPCTNCGMAPLPRGVARGKLNALSAGAEIIRGELSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="64" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LD94</accession>
    <name>Q1LD94_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative enzyme, thioesterase superfamily</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_5020</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the thioesterase PaaI family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11882.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519431.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5020"/>
    <dbReference type="EnsemblBacteria" id="ABF11882">
        <property type="protein sequence ID" value="ABF11882"/>
        <property type="gene ID" value="Rmet_5020"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5020"/>
    <dbReference type="eggNOG" id="COG2050">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_089876_8_1_4"/>
    <dbReference type="OrthoDB" id="1814691at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047617">
        <property type="term" value="F:acyl-CoA hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039298">
        <property type="entry name" value="ACOT13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029069">
        <property type="entry name" value="HotDog_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006683">
        <property type="entry name" value="Thioestr_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21660">
        <property type="entry name" value="PTHR21660"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03061">
        <property type="entry name" value="4HBT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54637">
        <property type="entry name" value="SSF54637"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="4HBT" evidence="2">
        <location>
            <begin position="58"/>
            <end position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF03061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF11882.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="143" mass="15407" checksum="364B0210187FC056" modified="2006-05-30" version="1">MSAPMLVLLPATSDTPHGFRRVRSSPGFMTTCGEFYVHESEPILATRITTAHLNAQDIVHGGFLATLADSAYGVVLRRTNPELIPRTAQLSVSYLGAVCEGDFVEARVTLHKIGKRLVNGSCDVYVGDRMVLQTTAVFSVVAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="104" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LDC8</accession>
    <name>Q1LDC8_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Two component transcriptional regulator, LuxR family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Rmet_4986</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="6">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="6">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11848.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519396.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4986"/>
    <dbReference type="EnsemblBacteria" id="ABF11848">
        <property type="protein sequence ID" value="ABF11848"/>
        <property type="gene ID" value="Rmet_4986"/>
    </dbReference>
    <dbReference type="GeneID" id="60823755"/>
    <dbReference type="KEGG" id="rme:Rmet_4986"/>
    <dbReference type="eggNOG" id="COG2197">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_90_10_4"/>
    <dbReference type="OMA" id="AEDQGMM"/>
    <dbReference type="OrthoDB" id="930905at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06170">
        <property type="entry name" value="LuxR_C_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016032">
        <property type="entry name" value="Sig_transdc_resp-reg_C-effctor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000792">
        <property type="entry name" value="Tscrpt_reg_LuxR_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00196">
        <property type="entry name" value="GerE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00038">
        <property type="entry name" value="HTHLUXR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00421">
        <property type="entry name" value="HTH_LUXR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46894">
        <property type="entry name" value="SSF46894"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00622">
        <property type="entry name" value="HTH_LUXR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50043">
        <property type="entry name" value="HTH_LUXR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="5" id="KW-0614">Plasmid</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Response regulatory" evidence="4">
        <location>
            <begin position="13"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="domain" description="HTH luxR-type" evidence="3">
        <location>
            <begin position="167"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="2">
        <location>
            <position position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABF11848.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="237" mass="25512" checksum="7474B4985C9B3EF9" modified="2006-05-30" version="1">MTHQPPAMDADIRVMLVEDDPATSARLTSALASVPGIHVMATCQNRVSACEWLTVNTPDVVLCDLGLPDGSGIDVIRFARRRHPHCECMVITMFGDEANVLASISAGATGYLLKDASDEDLRASVRELRAGGSPMSPVIARRVINRLRDANIPAPRAVRAEPATPLLPEAVVALSARESEILDLVARGYTYAEIARHLSISVNTVAAHIRNIYGKLAVNSRSEAVFEAHKLGLLKSM</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="97" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LDJ1</accession>
    <name>Q1LDJ1_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative thiol-disulfide isomerase/thioredoxin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_4923</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11785.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519346.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4923"/>
    <dbReference type="EnsemblBacteria" id="ABF11785">
        <property type="protein sequence ID" value="ABF11785"/>
        <property type="gene ID" value="Rmet_4923"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4923"/>
    <dbReference type="eggNOG" id="COG0526">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_090389_12_0_4"/>
    <dbReference type="OMA" id="YCQRAQP"/>
    <dbReference type="OrthoDB" id="1630944at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016853">
        <property type="term" value="F:isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00085">
        <property type="entry name" value="Thioredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="SSF52833"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0413">Isomerase</keyword>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thioredoxin" evidence="1">
        <location>
            <begin position="1"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF11785.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="108" mass="11998" checksum="E5FB300CD819C932" modified="2006-05-30" version="1">MSMTRSFAATEPTRDEIDALPGATLLEFGAPWCGYCMRAQPLIEEAFGGYPAIHHVKVEDGSGRPLGRSFRVKLWPTLVFLRDGREVARLVRPESAKDIAAAMRRILP</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LDX9</accession>
    <name>Q1LDX9_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 7">S-formylglutathione hydrolase</fullName>
            <ecNumber evidence="3 7">3.1.2.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">frmC</name>
        <name evidence="8" type="ordered locus">Rmet_4785</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="9">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="9">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Serine hydrolase involved in the detoxification of formaldehyde.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 7">
            <text>H2O + S-formylglutathione = formate + glutathione + H(+)</text>
            <dbReference type="Rhea" id="RHEA:14961"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15740"/>
            <dbReference type="ChEBI" id="CHEBI:57688"/>
            <dbReference type="ChEBI" id="CHEBI:57925"/>
            <dbReference type="EC" id="3.1.2.12"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the esterase D family.</text>
    </comment>
    <dbReference type="EC" id="3.1.2.12" evidence="3 7"/>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11647.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519215.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4785"/>
    <dbReference type="ESTHER" id="ralme-q1ldx9">
        <property type="family name" value="A85-EsteraseD-FGH"/>
    </dbReference>
    <dbReference type="MEROPS" id="S09.940"/>
    <dbReference type="EnsemblBacteria" id="ABF11647">
        <property type="protein sequence ID" value="ABF11647"/>
        <property type="gene ID" value="Rmet_4785"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4785"/>
    <dbReference type="eggNOG" id="COG0627">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_056472_0_0_4"/>
    <dbReference type="OMA" id="TFMEDHL"/>
    <dbReference type="OrthoDB" id="1828423at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052689">
        <property type="term" value="F:carboxylic ester hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018738">
        <property type="term" value="F:S-formylglutathione hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046294">
        <property type="term" value="P:formaldehyde catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000801">
        <property type="entry name" value="Esterase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014186">
        <property type="entry name" value="S-formylglutathione_hydrol"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10061">
        <property type="entry name" value="PTHR10061"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00756">
        <property type="entry name" value="Esterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="SSF53474"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02821">
        <property type="entry name" value="fghA_ester_D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0614">Plasmid</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0719">Serine esterase</keyword>
    <feature type="active site" description="Charge relay system" evidence="6">
        <location>
            <position position="150"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="6">
        <location>
            <position position="229"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="6">
        <location>
            <position position="262"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR614186-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU363068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ABF11647.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="282" mass="31010" checksum="3C67EABC043B9549" modified="2006-05-30" version="1">MELLSEHGCHGGVQRFYQHQSVAIGLPMRFSVYLPPQALQPGAKPLPALFYLAGLTCTEETFAIKANAQRFAAEHGLILVGPDTSPRGAHVPGEADAWDFGVGAGFYVDAVETPWRMHWRMESYVADELFQLVTRELPADAARVGIFGHSMGGHGALVLAQRFPQRFRSVSAFAPIAAPSDCPWGVKAFTGYLGEDRSRWAQHDASALMRAQTSAPFPAGILIDQGLADKFLAEQLHPEVFEAACEKAGQPLTLRRHDGYDHGYYFISTFIADHVRHHAEQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LE20</accession>
    <name>Q1LE20_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Arylamine N-acetyltransferase</fullName>
            <ecNumber evidence="3">2.3.1.5</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">nhoA</name>
        <name evidence="3" type="ordered locus">Rmet_4744</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the arylamine N-acetyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.5" evidence="3"/>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11606.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519179.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4744"/>
    <dbReference type="EnsemblBacteria" id="ABF11606">
        <property type="protein sequence ID" value="ABF11606"/>
        <property type="gene ID" value="Rmet_4744"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4744"/>
    <dbReference type="eggNOG" id="COG2162">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_049918_1_1_4"/>
    <dbReference type="OMA" id="CYEHNTL"/>
    <dbReference type="OrthoDB" id="1687886at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004060">
        <property type="term" value="F:arylamine N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001447">
        <property type="entry name" value="Arylamine_N-AcTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11786">
        <property type="entry name" value="PTHR11786"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00797">
        <property type="entry name" value="Acetyltransf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01543">
        <property type="entry name" value="ANATRNSFRASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="SSF54001"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006547"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU003452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF11606.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="292" mass="31626" checksum="231B76651FCFADE4" modified="2006-05-30" version="1">MLAADEIPALHPDQLVAWLRRIGIAEVPDAPTLPVLNTLIAAQLAHIPFENLDALLGRRVSIDLPSVFEKLVAQGRGGYCFEQNTLLCAGLKALGYAVTPLAARVRWHVPEATPTGLSHMLLRVEVAHESYIADVGFGGPTPDRALSLSLPPDENTPYRLHPSPANALTGTGFHCLDLTVPDVSDDASGWQPIYRFDLTPQPWIDFIARNWYVSTHPDSHFTRTLMAARTDGDTRLALANGNLSERSPDGSVRKTALASADAVIDTLATRFQIRLDPALRAALATRLPAVLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LEC3</accession>
    <name>Q1LEC3_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">ATP-dependent endonuclease of the OLD family-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_4641</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11503.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519078.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4641"/>
    <dbReference type="EnsemblBacteria" id="ABF11503">
        <property type="protein sequence ID" value="ABF11503"/>
        <property type="gene ID" value="Rmet_4641"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4641"/>
    <dbReference type="eggNOG" id="COG0419">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG3593">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017618_1_0_4"/>
    <dbReference type="OMA" id="EPELYIH"/>
    <dbReference type="OrthoDB" id="318943at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01026">
        <property type="entry name" value="TOPRIM_OLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041685">
        <property type="entry name" value="AAA_15"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034139">
        <property type="entry name" value="TOPRIM_OLD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13175">
        <property type="entry name" value="AAA_15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0540">Nuclease</keyword>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA_15" evidence="1">
        <location>
            <begin position="1"/>
            <end position="383"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF11503.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="626" mass="68130" checksum="A6750C3BEE2C180A" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="105" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LED5</accession>
    <name>Q1LED5_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Transcriptional regulator, AraC family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Rmet_4629</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="7">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="7">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11491.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519067.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4629"/>
    <dbReference type="EnsemblBacteria" id="ABF11491">
        <property type="protein sequence ID" value="ABF11491"/>
        <property type="gene ID" value="Rmet_4629"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4629"/>
    <dbReference type="eggNOG" id="COG2207">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047522_1_2_4"/>
    <dbReference type="OMA" id="HCERELA"/>
    <dbReference type="OrthoDB" id="1622875at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032687">
        <property type="entry name" value="AraC-type_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018060">
        <property type="entry name" value="HTH_AraC"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12625">
        <property type="entry name" value="Arabinose_bd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12833">
        <property type="entry name" value="HTH_18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00342">
        <property type="entry name" value="HTH_ARAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01124">
        <property type="entry name" value="HTH_ARAC_FAMILY_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0614">Plasmid</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH araC/xylS-type" evidence="5">
        <location>
            <begin position="239"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="338"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="338"/>
            <end position="355"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS01124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABF11491.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="363" mass="40099" checksum="503D2C51EBE93592" modified="2006-05-30" version="1">MTAPSIQQFRLVRSASLNGYIELVKSLGYDPRAFLRGVGLSARLLENPETLIPSPAVRELLEVTAQVTGVEDFALRLAARRTFSNLGPISLVLKEAATPREALDTLCRYLKLLSAALITRVEDVGDAVIIREDLLPTPGLSSRQALELAMGVTFRMLRELIGPRWRPQQVCFMHRPPADLSPYRAFFGRNPMFNQPFNGMVCAAADLSMSRMPDDPGVARFARDYLEAALRHREIGIRDSCRELVQALLPGGRCTADQVARHLGVDRRTLHRYLSAEGLTFSTLLHEVRSELVMRHLLESDLPMGEVAGLLGFSAQSSFSHWFQTVFGCSATDWRRVQSAQPGKSGKSGQSAAATGRRAPSRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LEI1</accession>
    <name>Q1LEI1_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Glutamate/aspartate transport protein (ABC superfamily, peri_bind) high affinity periplasmic solute-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">gltI</name>
        <name evidence="4" type="ordered locus">Rmet_4583</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="5">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="5">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11445.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519030.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4583"/>
    <dbReference type="EnsemblBacteria" id="ABF11445">
        <property type="protein sequence ID" value="ABF11445"/>
        <property type="gene ID" value="Rmet_4583"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4583"/>
    <dbReference type="eggNOG" id="COG0834">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019602_0_0_4"/>
    <dbReference type="OMA" id="DCAPNTN"/>
    <dbReference type="OrthoDB" id="685252at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001638">
        <property type="entry name" value="Solute-binding_3/MltF_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00497">
        <property type="entry name" value="SBP_bac_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00062">
        <property type="entry name" value="PBPb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0614">Plasmid</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193463" evidence="2">
        <location>
            <begin position="30"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="domain" description="PBPb" evidence="3">
        <location>
            <begin position="45"/>
            <end position="275"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00062"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF11445.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="304" mass="33077" checksum="8AAFA66CA3E6C00C" modified="2006-05-30" version="1">MPSIVSVSSLQRLLAGIALAAALVPAAHAQNPQSPVLDRIRDSGTITLGYRESALPFSFGDDKGQPAGYAVDLCLRVAEAAKEKLGLKTIKVRWLPLTPQNRVPAVVNGLVDLDCAPNTNTLERQKQVAFSVSHYVSTVRMLVRADSGIKSFDDLRGKTVVTSAGSTGDRHVRRLKAEYGYRDVYAKDHGESFLLLETGRAQAFVMDDVLLAGLRAKSKDPSQYVIVGPALSVEQNALMLSKADPEWKRLVDQTLAKTFTSPDVAAMQKRWFQQPIGTRGTNLALAPSTEVLQAWKHPSDVVTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-10-07" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LF38</accession>
    <name>Q1LF38_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Transcriptional regulator, XRE family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_4373</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11238.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4373"/>
    <dbReference type="EnsemblBacteria" id="ABF11238">
        <property type="protein sequence ID" value="ABF11238"/>
        <property type="gene ID" value="Rmet_4373"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4373"/>
    <dbReference type="eggNOG" id="COG3620">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2154181_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00093">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001387">
        <property type="entry name" value="Cro/C1-type_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010982">
        <property type="entry name" value="Lambda_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="SMART" id="SM00530">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47413">
        <property type="entry name" value="SSF47413"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50943">
        <property type="entry name" value="HTH_CROC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH cro/C1-type" evidence="2">
        <location>
            <begin position="17"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="93"/>
            <end position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF11238.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="123" mass="12637" checksum="59859A14B51DF4C6" modified="2006-05-30" version="1">MNSTAVPRGASEPFRQLRALRRARKLKQEDVARKAGISREAYLRAESGQADPRMSTFLAACEALGLEVVLAPQHLAADVNAFIASRNGGVTAASMAGPATTAPAAPAPGGFGSGSTPEGHKPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LF87</accession>
    <name>Q1LF87_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Cytochrome c oxidase, cbb3-type, subunit III</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">ccoP</name>
        <name evidence="8" type="ordered locus">Rmet_4324</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="9">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="9">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11189.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518813.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4324"/>
    <dbReference type="EnsemblBacteria" id="ABF11189">
        <property type="protein sequence ID" value="ABF11189"/>
        <property type="gene ID" value="Rmet_4324"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4324"/>
    <dbReference type="eggNOG" id="COG2010">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_093848_1_0_4"/>
    <dbReference type="OMA" id="VRSQWGN"/>
    <dbReference type="OrthoDB" id="1939800at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.760.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009056">
        <property type="entry name" value="Cyt_c-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036909">
        <property type="entry name" value="Cyt_c-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008168">
        <property type="entry name" value="Cyt_C_IC"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13442">
        <property type="entry name" value="Cytochrome_CBB3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00605">
        <property type="entry name" value="CYTCHROMECIC"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46626">
        <property type="entry name" value="SSF46626"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51007">
        <property type="entry name" value="CYTC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 4" id="KW-0349">Heme</keyword>
    <keyword evidence="3 4" id="KW-0408">Iron</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="2 4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0614">Plasmid</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="31"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="domain" description="Cytochrome c" evidence="7">
        <location>
            <begin position="75"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ABF11189.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="202" mass="20931" checksum="281C163B3F980549" modified="2006-05-30" version="1">MTDVRETRQAEAARAREHEDPHENQAPIPRYVLAMVAVLVAWGAWYIATAPINQAPELGDRRTLADLRGNAKGAGAKVDGAAIFQSRCVACHQSNGQGLPGVFPPLAGSEWVNGNESRVARIVLRGVTGKLTVKGAVYNGAMPAFADQLTDAEIAAVLTHVRVQWGNSASAISAEAVAASRADTAGMKGSFDGDAALGGSGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LFE2</accession>
    <name>Q1LFE2_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Citrate synthase (unknown stereospecificity)</fullName>
            <ecNumber evidence="2">2.3.3.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">citA</name>
        <name evidence="3" type="ordered locus">Rmet_4268</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Carbohydrate metabolism; tricarboxylic acid cycle; isocitrate from oxaloacetate: step 1/2.</text>
    </comment>
    <dbReference type="EC" id="2.3.3.16" evidence="2"/>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11134.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4268"/>
    <dbReference type="EnsemblBacteria" id="ABF11134">
        <property type="protein sequence ID" value="ABF11134"/>
        <property type="gene ID" value="Rmet_4268"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4268"/>
    <dbReference type="eggNOG" id="COG0372">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_070533_1_0_4"/>
    <dbReference type="OMA" id="YGHPLHK"/>
    <dbReference type="UniPathway" id="UPA00223">
        <property type="reaction ID" value="UER00717"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036440">
        <property type="term" value="F:citrate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.580.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016142">
        <property type="entry name" value="Citrate_synth-like_lrg_a-sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016143">
        <property type="entry name" value="Citrate_synth-like_sm_a-sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002020">
        <property type="entry name" value="Citrate_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036969">
        <property type="entry name" value="Citrate_synthase_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00285">
        <property type="entry name" value="Citrate_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48256">
        <property type="entry name" value="SSF48256"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004751"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012972"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF11134.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="265" mass="27469" checksum="278F15B437CF7F75" modified="2006-05-30" version="1">MKASSPDTKPPATAIAFVDMHGVSVHGRNLSRDLIGHMGFTEYFLFLLTSRVPDSGLVAITNACLVAIAEHGLVPSVQAARMTLAAAPDALQGAVAAGLLGCGSVILGASQTAGLMLQEVLTAQGERSLEESADTVVATLRRERKPLPGFGHPTHKEGDPRAHRLLEVSRELGLAGRHVAALEAVAAAVPRHFPRPIPLNVSGAIPAVLLDAGYPVGALKGVPLLARVASLIAHLQEETRLPIGFALADAAEHAVAYIGPEVCTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LFS6</accession>
    <name>Q1LFS6_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative oxidoreductase (Puryvate dehydrogenase E1 component, beta subunit)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">pdhB</name>
        <name evidence="2" type="ordered locus">Rmet_4133</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11000.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518625.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4133"/>
    <dbReference type="EnsemblBacteria" id="ABF11000">
        <property type="protein sequence ID" value="ABF11000"/>
        <property type="gene ID" value="Rmet_4133"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4133"/>
    <dbReference type="eggNOG" id="COG0022">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012907_1_0_4"/>
    <dbReference type="OMA" id="LPLDTCF"/>
    <dbReference type="OrthoDB" id="900125at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009014">
        <property type="entry name" value="Transketo_C/PFOR_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005475">
        <property type="entry name" value="Transketolase-like_Pyr-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033248">
        <property type="entry name" value="Transketolase_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02779">
        <property type="entry name" value="Transket_pyr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02780">
        <property type="entry name" value="Transketolase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00861">
        <property type="entry name" value="Transket_pyr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="SSF52518"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52922">
        <property type="entry name" value="SSF52922"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transket_pyr" evidence="1">
        <location>
            <begin position="4"/>
            <end position="179"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00861"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF11000.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="326" mass="34389" checksum="0D6B65EE6E0ACB57" modified="2006-05-30" version="1">MAELHMVEAVNLALAHALANDPDVVLLGEDIGVNGGVFRSTVGLQSRFGEARVIDTPLAEGAIVGAAIGMAAMGLKPVAEIQFAGFIYPAIDNILNHAGHMRHRTRGRITCPMVVRAPSGAGIHAPEHHSESPEALFAQMPGIRVVMPSSPARAYGLLLAAIRDPDPVIFLEPTRLYRLFRQEVADDGQALPLDACFTLREGTDLTLVSWGAMLRETLAAADVLADDGVSAAVIDVATLKPLDMETILESVAQSGRCVIVHEAPRTAGLGAEIAANLADAGLYSLSAPVQRVTAPDTVVPLARLEHSYMPSVARIVDAARRALEAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LFW2</accession>
    <name>Q1LFW2_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Outer membrane porin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Rmet_4097</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="8">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="8">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10964.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518591.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4097"/>
    <dbReference type="EnsemblBacteria" id="ABF10964">
        <property type="protein sequence ID" value="ABF10964"/>
        <property type="gene ID" value="Rmet_4097"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4097"/>
    <dbReference type="eggNOG" id="COG3203">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038238_1_1_4"/>
    <dbReference type="OMA" id="VYGHYAM"/>
    <dbReference type="OrthoDB" id="1064531at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046930">
        <property type="term" value="C:pore complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015288">
        <property type="term" value="F:porin activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006811">
        <property type="term" value="P:ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00342">
        <property type="entry name" value="gram_neg_porins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.160.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033900">
        <property type="entry name" value="Gram_neg_porin_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023614">
        <property type="entry name" value="Porin_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002299">
        <property type="entry name" value="Porin_Neis"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13609">
        <property type="entry name" value="Porin_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00184">
        <property type="entry name" value="NEISSPPORIN"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0406">Ion transport</keyword>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-0626">Porin</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1134">Transmembrane beta strand</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193421" evidence="5">
        <location>
            <begin position="33"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="domain" description="Porin_4" evidence="6">
        <location>
            <begin position="23"/>
            <end position="344"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF13609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABF10964.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="374" mass="40454" checksum="EBC495C06B9403C4" modified="2006-05-30" version="1">MTRVGVWHWMRPLATIAALSASATCLVGTANAQSSVVIYGRLNTAVEYASASTATDGTHLGGTARLTNNRSVFGIRGEEALGYGLKAIWQVESTLSIDTGAGQIAARDTRLGMASPYGTVFMGNWDTAYTTSTKTLDPYYPTTAGYMSIMGNGAAATTDNVQNTNSFDRRQQNSLHYQSPQWHGLSGAMTWGLPEEKISVPRNPALYSFAGAYERGPLSVSMAYEIHQHYQTAGRNDDGMKVGVSYQFPTTTIALVYERLHYRTATGDLARNSYYTSLVQKLGPGSVKIGFTFASNGTGNATETIGSFHAGPETGATQITVGYDYPLSKRTSIYTYYSRINNKRNALYDFAINGLGVKAGADPQTFALAMRHNF</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="102" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LG31</accession>
    <name>Q1LG31_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 6 8">Proline iminopeptidase</fullName>
            <shortName evidence="6">PIP</shortName>
            <ecNumber evidence="4 6 8">3.4.11.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Prolyl aminopeptidase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">Rmet_4027</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="11">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="11">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 6 8">
            <text>Release of N-terminal proline from a peptide.</text>
            <dbReference type="EC" id="3.4.11.5"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 6 8">Belongs to the peptidase S33 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.11.5" evidence="4 6 8"/>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10895.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518534.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4027"/>
    <dbReference type="ESTHER" id="ralme-q1lg31">
        <property type="family name" value="Proline_iminopeptidase"/>
    </dbReference>
    <dbReference type="MEROPS" id="S33.001"/>
    <dbReference type="EnsemblBacteria" id="ABF10895">
        <property type="protein sequence ID" value="ABF10895"/>
        <property type="gene ID" value="Rmet_4027"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4027"/>
    <dbReference type="eggNOG" id="COG0596">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_043739_2_2_4"/>
    <dbReference type="OMA" id="FYQDGAS"/>
    <dbReference type="OrthoDB" id="665798at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004177">
        <property type="term" value="F:aminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002410">
        <property type="entry name" value="Peptidase_S33"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005944">
        <property type="entry name" value="Pro_iminopeptidase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43722">
        <property type="entry name" value="PTHR43722"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00561">
        <property type="entry name" value="Abhydrolase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006431">
        <property type="entry name" value="Pept_S33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00111">
        <property type="entry name" value="ABHYDROLASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00793">
        <property type="entry name" value="PROAMNOPTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="SSF53474"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01249">
        <property type="entry name" value="pro_imino_pep_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8 10" id="KW-0031">Aminopeptidase</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 8 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-0614">Plasmid</keyword>
    <keyword evidence="6 8" id="KW-0645">Protease</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AB hydrolase-1" evidence="9">
        <location>
            <begin position="41"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="7">
        <location>
            <position position="113"/>
        </location>
    </feature>
    <feature type="active site" evidence="7">
        <location>
            <position position="268"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="7">
        <location>
            <position position="296"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001585"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010088"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00021843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR006431"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR006431-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003421"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ABF10895.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="318" mass="35515" checksum="99542FD0720D0B59" modified="2006-05-30" version="1">MPEPRTLYPAIEPYETGTLDVGDGHVLYYERVGTRGAKPAVFLHGGPGGGISPDHRRLFDPARYDVLLFDQRGCGQSTPHAELEANTTWHLVDDIEKLRERIGVERWLVFGGSWGSTLALAYAQTHPQRVSELVLRGVYTVSRAEVDWYYQFGVSEMFPDKWARFQAPVPEAERGNMMAAYRKLLTGDDKAKQVEAAQAWSVWEGETITLLPDAGISTKHADGHFALAFARIENHYFTHGAWLDDGQLLRDAHRLAGIPGVIVHGRYDMPCPVRYAYALHQAWPDADFHLIEGAGHAWSEPGILDQLIAATDHFAGKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="66" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LGF2</accession>
    <name>Q1LGF2_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_3906</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10774.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518420.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3906"/>
    <dbReference type="EnsemblBacteria" id="ABF10774">
        <property type="protein sequence ID" value="ABF10774"/>
        <property type="gene ID" value="Rmet_3906"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3906"/>
    <dbReference type="eggNOG" id="COG2719">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010179_3_0_4"/>
    <dbReference type="OMA" id="NSNPCYA"/>
    <dbReference type="OrthoDB" id="507759at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007390">
        <property type="entry name" value="Spore_V_R"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30029">
        <property type="entry name" value="PTHR30029"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04293">
        <property type="entry name" value="SpoVR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF10774.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="512" mass="60738" checksum="DE6B5EC7FC67F555" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="67" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LGM9</accession>
    <name>Q1LGM9_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_3827</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10697.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518343.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3827"/>
    <dbReference type="EnsemblBacteria" id="ABF10697">
        <property type="protein sequence ID" value="ABF10697"/>
        <property type="gene ID" value="Rmet_3827"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3827"/>
    <dbReference type="eggNOG" id="COG0220">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_067698_2_0_4"/>
    <dbReference type="OMA" id="NPEWGVR"/>
    <dbReference type="OrthoDB" id="1515497at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010342">
        <property type="entry name" value="DUF938"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20974">
        <property type="entry name" value="PTHR20974"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06080">
        <property type="entry name" value="DUF938"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF10697.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="204" mass="22099" checksum="D63975526B857194" modified="2006-05-30" version="1">MTADPTARRMAPATDRNREPILAVLRNVLPRSGTVLEIASGTGQHAVHFAAALPGLTWQPSDLDATHRESIAAWTEHTQVQNVLPPLAIDVRQEPWGIDAVDAIVCINMIHIAPWAAAEALFEGAGRRLAPGGVLYLYGPYRREGAHTAPSNEAFDRQLHSADSDWGVRDMEAVIALGTTHGLTCDAPIQMPANNFSLVFRKQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="104" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LH22</accession>
    <name>Q1LH22_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Methyl-accepting chemotaxis sensory transducer with Pas/Pac sensor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">aer</name>
        <name evidence="9" type="ordered locus">Rmet_3682</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="10">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="10">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the methyl-accepting chemotaxis (MCP) protein family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10554.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518205.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3682"/>
    <dbReference type="EnsemblBacteria" id="ABF10554">
        <property type="protein sequence ID" value="ABF10554"/>
        <property type="gene ID" value="Rmet_3682"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3682"/>
    <dbReference type="eggNOG" id="COG0840">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_107_26_4"/>
    <dbReference type="OMA" id="AVFEHMW"/>
    <dbReference type="OrthoDB" id="429006at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004888">
        <property type="term" value="F:transmembrane signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006935">
        <property type="term" value="P:chemotaxis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00130">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004090">
        <property type="entry name" value="Chemotax_Me-accpt_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003660">
        <property type="entry name" value="HAMP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004089">
        <property type="entry name" value="MCPsignal_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000014">
        <property type="entry name" value="PAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035965">
        <property type="entry name" value="PAS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013655">
        <property type="entry name" value="PAS_fold_3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00672">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00015">
        <property type="entry name" value="MCPsignal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08447">
        <property type="entry name" value="PAS_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00260">
        <property type="entry name" value="CHEMTRNSDUCR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00304">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00283">
        <property type="entry name" value="MA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55785">
        <property type="entry name" value="SSF55785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00229">
        <property type="entry name" value="sensory_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50111">
        <property type="entry name" value="CHEMOTAXIS_TRANSDUC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50885">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50112">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0614">Plasmid</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0807">Transducer</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="169"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="201"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="domain" description="PAS" evidence="7">
        <location>
            <begin position="25"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="domain" description="HAMP" evidence="8">
        <location>
            <begin position="222"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="domain" description="Methyl-accepting transducer" evidence="6">
        <location>
            <begin position="279"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="532"/>
            <end position="552"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="479"/>
            <end position="517"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00029447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ABF10554.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="552" mass="59457" checksum="BA4A651C7B61BD0B" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="66" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LH44</accession>
    <name>Q1LH44_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_3660</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10532.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518187.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3660"/>
    <dbReference type="EnsemblBacteria" id="ABF10532">
        <property type="protein sequence ID" value="ABF10532"/>
        <property type="gene ID" value="Rmet_3660"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3660"/>
    <dbReference type="eggNOG" id="ENOG50339NA">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2106993_0_0_4"/>
    <dbReference type="OMA" id="YTGQANA"/>
    <dbReference type="OrthoDB" id="2085303at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022053">
        <property type="entry name" value="DUF3613"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12266">
        <property type="entry name" value="DUF3613"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193539" evidence="2">
        <location>
            <begin position="33"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="30"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="103"/>
            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF10532.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="122" mass="12615" checksum="8D4D93ABD0A87BD9" modified="2006-05-30" version="1">MTRNRSRLTDAACAVAISAMLLGAAGAAGAQAAQSTQAVEPPPSPTFHPNDVPVGDTTRMLLEVQRNGSQAGPQQSMTGEEAAIGYARYMRSFQYPLPEFFSSQSTGSALRGSTGSPAPVGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LH61</accession>
    <name>Q1LH61_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Transcriptional regulator, MarR family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_3643</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10515.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3643"/>
    <dbReference type="EnsemblBacteria" id="ABF10515">
        <property type="protein sequence ID" value="ABF10515"/>
        <property type="gene ID" value="Rmet_3643"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3643"/>
    <dbReference type="eggNOG" id="COG1846">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_120009_1_0_4"/>
    <dbReference type="OMA" id="PGREWAT"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000835">
        <property type="entry name" value="HTH_MarR-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12802">
        <property type="entry name" value="MarR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00347">
        <property type="entry name" value="HTH_MARR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50995">
        <property type="entry name" value="HTH_MARR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH marR-type" evidence="2">
        <location>
            <begin position="32"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF10515.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="169" mass="19024" checksum="D7857D51E247F701" modified="2006-05-30" version="1">MGGRSTNALADPTSMPRKSAQARASEETAVLHKQDFEALSDFRYQLRRFLRFSEEAARAEGVTVQQYLLMLHIRGCPDKNWASVGELAERLQAKQHGVVALVNRCEAAGLVSRKVNPDDRRVVQIHLLAKGERCLNRLAALHRTELESLRNTFRVARITAFNDDGAERI</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="65" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LH73</accession>
    <name>Q1LH73_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_3631</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10503.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008646896.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3631"/>
    <dbReference type="EnsemblBacteria" id="ABF10503">
        <property type="protein sequence ID" value="ABF10503"/>
        <property type="gene ID" value="Rmet_3631"/>
    </dbReference>
    <dbReference type="GeneID" id="60826187"/>
    <dbReference type="KEGG" id="rme:Rmet_3631"/>
    <dbReference type="eggNOG" id="COG3193">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_103773_2_0_4"/>
    <dbReference type="OMA" id="RMDDAWV"/>
    <dbReference type="OrthoDB" id="1551899at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005624">
        <property type="entry name" value="PduO/GlcC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038084">
        <property type="entry name" value="PduO/GlcC-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03928">
        <property type="entry name" value="Haem_degrading"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143744">
        <property type="entry name" value="SSF143744"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF10503.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="159" mass="16740" checksum="543D5FD40C832B17" modified="2006-05-30" version="1">MKKAIKLELKEARHMVAAAIRKAEEIGVLESICIVDDGGYPLLLERMDGARITGPQIAWNKAFTAAGHKRSTHLFNTPPNGPALPGNEAFGIQWSFDGKFAVFVGGYPIVVNGEVIGGVGLSGGNGEQDTACGVAALEALRDLLAAEDLTVLAQADIKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LHA9</accession>
    <name>Q1LHA9_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Transcriptional regulator, TetR-family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Rmet_3595</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10467.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518010.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3595"/>
    <dbReference type="EnsemblBacteria" id="ABF10467">
        <property type="protein sequence ID" value="ABF10467"/>
        <property type="gene ID" value="Rmet_3595"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3595"/>
    <dbReference type="eggNOG" id="COG1309">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069356_19_0_4"/>
    <dbReference type="OMA" id="IRCEHVM"/>
    <dbReference type="OrthoDB" id="2089619at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023772">
        <property type="entry name" value="DNA-bd_HTH_TetR-type_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001647">
        <property type="entry name" value="HTH_TetR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041586">
        <property type="entry name" value="PsrA_TetR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036271">
        <property type="entry name" value="Tet_transcr_reg_TetR-rel_C_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17939">
        <property type="entry name" value="TetR_C_30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00440">
        <property type="entry name" value="TetR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00455">
        <property type="entry name" value="HTHTETR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48498">
        <property type="entry name" value="SSF48498"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01081">
        <property type="entry name" value="HTH_TETR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50977">
        <property type="entry name" value="HTH_TETR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0678">Repressor</keyword>
    <feature type="domain" description="HTH tetR-type" evidence="3">
        <location>
            <begin position="9"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="H-T-H motif" evidence="2">
        <location>
            <begin position="32"/>
            <end position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF10467.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="313" mass="34185" checksum="818C74DAB46123B1" modified="2006-05-30" version="1">MKTGKTTAGDTKARILDATEKLFIEVGYEATSLRQVTSRAIVNLAAVNYHFRSKEIMMQSVLGRRLDPLNTRRLALLDACEARWPGAEIRCEHVMGALFVPALQMAREPEIGGPSFLRLLGRVYSDTSPFIQSWLMGHYAPVFGRFFEAFAHALPDVPRQELGWRLHFALKALAGVLAGDELGNLMPTFTQGKRMSDAQVLAQLTSMVVAALKAPSPAPQELGALQDVFEIGETQHADEQAHLAALAATVEAESHEAAAAAAAALGRARRSARQVRNEGATRAATASMAVRREHCATFPSNPLDDWMRTRPRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="64" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LHP4</accession>
    <name>Q1LHP4_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Rmet_3460</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the UPF0719 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10332.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517890.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3460"/>
    <dbReference type="EnsemblBacteria" id="ABF10332">
        <property type="protein sequence ID" value="ABF10332"/>
        <property type="gene ID" value="Rmet_3460"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3460"/>
    <dbReference type="eggNOG" id="COG3766">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_122820_0_0_4"/>
    <dbReference type="OMA" id="VLNAACM"/>
    <dbReference type="OrthoDB" id="1807657at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007140">
        <property type="entry name" value="DUF350"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03994">
        <property type="entry name" value="DUF350"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="42"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="72"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="106"/>
            <end position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005779"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ABF10332.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="128" mass="13539" checksum="D5271B11D4BB2FCF" modified="2006-05-30" version="1">MLPIYAYAEHLLASLVLLVVFVGVYTRITPFNEFALIRQGNVAAALSLGGSTLGFCFTLSSSLQHNDTFLMFLVWSLVAMVVQAVVYAALARALPDMDAAIESNNMAMGGLMGTMSVSVGVLNAACLS</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-10-03" modified="2022-05-25" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LI56</accession>
    <name>RL15_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">50S ribosomal protein L15</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rplO</name>
        <name type="ordered locus">Rmet_3298</name>
    </gene>
    <organism>
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Binds to the 23S rRNA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the 50S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL15 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10170.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008642954.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q1LI56"/>
    <dbReference type="SMR" id="Q1LI56"/>
    <dbReference type="STRING" id="266264.Rmet_3298"/>
    <dbReference type="EnsemblBacteria" id="ABF10170">
        <property type="protein sequence ID" value="ABF10170"/>
        <property type="gene ID" value="Rmet_3298"/>
    </dbReference>
    <dbReference type="GeneID" id="60826619"/>
    <dbReference type="KEGG" id="rme:Rmet_3298"/>
    <dbReference type="eggNOG" id="COG0200">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_055188_4_2_4"/>
    <dbReference type="OMA" id="FKNINRV"/>
    <dbReference type="OrthoDB" id="1874945at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015934">
        <property type="term" value="C:large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01341">
        <property type="entry name" value="Ribosomal_L15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036227">
        <property type="entry name" value="L18e/L15P_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030878">
        <property type="entry name" value="Ribosomal_L15"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005749">
        <property type="entry name" value="Ribosomal_L15_bac-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021131">
        <property type="entry name" value="Ribosomal_L18e/L15P"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12934">
        <property type="entry name" value="PTHR12934"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00828">
        <property type="entry name" value="Ribosomal_L27A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52080">
        <property type="entry name" value="SSF52080"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01071">
        <property type="entry name" value="rplO_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <feature type="chain" id="PRO_0000251545" description="50S ribosomal protein L15">
        <location>
            <begin position="1"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="48"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01341"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="144" mass="15058" checksum="293772C0A1816482" modified="2006-05-30" version="1">MQLNNLKPADGSKHAKRRVGRGIGSGLGKTAGRGHKGQKSRSGGFHKVGFEGGQMPLYRRLPKRGFTSLTKAFTAEVTLRDIERLEAAEVDLLVLKQAGLVGELVKSAKVIKAGELSRKVTIKGLGATAGAKAAIEAAGGQIEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LIC2</accession>
    <name>Q1LIC2_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_3232</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the GTP cyclohydrolase I type 2/NIF3 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10104.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517714.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3232"/>
    <dbReference type="EnsemblBacteria" id="ABF10104">
        <property type="protein sequence ID" value="ABF10104"/>
        <property type="gene ID" value="Rmet_3232"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3232"/>
    <dbReference type="eggNOG" id="COG0327">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037423_3_0_4"/>
    <dbReference type="OMA" id="HGLFWRG"/>
    <dbReference type="OrthoDB" id="502462at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002678">
        <property type="entry name" value="DUF34/NIF3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036069">
        <property type="entry name" value="DUF34/NIF3_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13799">
        <property type="entry name" value="PTHR13799"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01784">
        <property type="entry name" value="NIF3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102705">
        <property type="entry name" value="SSF102705"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00486">
        <property type="entry name" value="YbgI_SA1388"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF10104.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="248" mass="26749" checksum="AD7A892EAB38AA27" modified="2006-05-30" version="1">MKTKELELYLNDLLQVSRFKDYCPNGLQVQGRPEVAHIVTGVTASLALIDAAIEAGADAILVHHGYFWKNEDACIVGQKHARLKRLLGADINLLAYHLPLDSHPELGNNAQLGLQLGITPTGRFSDDQLGWTGTLPQPMPLSAFAAHVGGVLEREPLVIGDADQMIRTVGWCTGGAQGYFAAAVDAGVDLYMSGEVSEPTTHLARESGVAYLAAGHHATERYGIRAVGEHLAQRFGLRHTFIDIPNPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LIR9</accession>
    <name>Q1LIR9_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative NADPH:quinone reductase or related Zn-dependent oxidoreductase</fullName>
            <ecNumber evidence="2">1.6.5.5</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_3085</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EC" id="1.6.5.5" evidence="2"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09957.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517585.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3085"/>
    <dbReference type="EnsemblBacteria" id="ABF09957">
        <property type="protein sequence ID" value="ABF09957"/>
        <property type="gene ID" value="Rmet_3085"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3085"/>
    <dbReference type="eggNOG" id="COG0604">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026673_7_0_4"/>
    <dbReference type="OMA" id="NPGMSSW"/>
    <dbReference type="OrthoDB" id="831053at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003960">
        <property type="term" value="F:NADPH:quinone reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020843">
        <property type="entry name" value="PKS_ER"/>
    </dbReference>
    <dbReference type="SMART" id="SM00829">
        <property type="entry name" value="PKS_ER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="SSF50129"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PKS_ER" evidence="1">
        <location>
            <begin position="10"/>
            <end position="317"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF09957.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="320" mass="33718" checksum="1BAC0E64501514D6" modified="2006-05-30" version="1">MKAVVQHQFGETPCFTTFDDPYPSDGETLVQVRAAVICPADLAIAKGLHPRLPTQLPFICGRDGVGRLADGQRVYFRASRAPYGAMAEYAPAEWVAPLPDDVGWAAAAALVGPALAAWLPLATRADVEPGETVLVLGASGIAGRLAVQAARLQGAGRVIAAGHLPEQLSTLGADALIDLALPPSILQYTFASFAEQGIDVVIDFVWGEALELLLAALVQCDGMPMSMFDRGVRIVTTANRGAGSMMLPPAALRDSRVQLMGCGPANHPPLGYMKTLVDDILACASDSDLGTEFELAQMSAVADVWRRAGEQATRVVLTLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LIZ2</accession>
    <name>Q1LIZ2_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">RND divalent metal cation efflux transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">hmzA</name>
        <name evidence="4" type="ordered locus">Rmet_3011</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the resistance-nodulation-cell division (RND) (TC 2.A.6) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09884.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3011"/>
    <dbReference type="EnsemblBacteria" id="ABF09884">
        <property type="protein sequence ID" value="ABF09884"/>
        <property type="gene ID" value="Rmet_3011"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3011"/>
    <dbReference type="eggNOG" id="COG3696">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002755_1_2_4"/>
    <dbReference type="OMA" id="GDNVIFW"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008324">
        <property type="term" value="F:cation transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2090.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027463">
        <property type="entry name" value="AcrB_DN_DC_subdom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001036">
        <property type="entry name" value="Acrflvin-R"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004763">
        <property type="entry name" value="CzcA/CusA/SilA/NccA/HelA/CnrA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32063">
        <property type="entry name" value="PTHR32063"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00873">
        <property type="entry name" value="ACR_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00702">
        <property type="entry name" value="ACRIFLAVINRP"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82714">
        <property type="entry name" value="SSF82714"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00914">
        <property type="entry name" value="2A0601"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="346"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="391"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="439"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="467"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="528"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="864"/>
            <end position="883"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="889"/>
            <end position="909"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF09884.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="1009" mass="107410" checksum="235C6C38F7BE25BA" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="63" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LJ18</accession>
    <name>Q1LJ18_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative barstar (Ribonuclease inhibitor)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_2985</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the barstar family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09858.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517519.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2985"/>
    <dbReference type="EnsemblBacteria" id="ABF09858">
        <property type="protein sequence ID" value="ABF09858"/>
        <property type="gene ID" value="Rmet_2985"/>
    </dbReference>
    <dbReference type="GeneID" id="60820578"/>
    <dbReference type="KEGG" id="rme:Rmet_2985"/>
    <dbReference type="eggNOG" id="COG2732">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_121832_0_0_4"/>
    <dbReference type="OMA" id="HFLYANC"/>
    <dbReference type="OrthoDB" id="2047621at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.370.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000468">
        <property type="entry name" value="Barstar"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035905">
        <property type="entry name" value="Barstar-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01337">
        <property type="entry name" value="Barstar"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52038">
        <property type="entry name" value="SSF52038"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Barstar" evidence="2">
        <location>
            <begin position="107"/>
            <end position="191"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01337"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF09858.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="202" mass="22584" checksum="71B7D19F38FCD6B7" modified="2006-05-30" version="1">MMTDIFGLGDALAAREERGAGDGWQRAHHQAQNLYDNVLMMPRQDLPHRLPPACAPAMVACDDNGTNEGAMNLFKTVRPNIVQSIRAFRVPELAQAAAALNQHFLYANCADCASKAEILERIATEFTFPKHFGKNFDALSDCLTDMIYKAGPQPGFVIVLEGLPIAQKFDKEAREVLLDVFRDAAEFWGERKVQFRVFYSFA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LJ25</accession>
    <name>Q1LJ25_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Flavin reductase-like, FMN-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_2978</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09851.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517513.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2978"/>
    <dbReference type="EnsemblBacteria" id="ABF09851">
        <property type="protein sequence ID" value="ABF09851"/>
        <property type="gene ID" value="Rmet_2978"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2978"/>
    <dbReference type="eggNOG" id="COG1853">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_059021_5_4_4"/>
    <dbReference type="OMA" id="WAMPLDF"/>
    <dbReference type="OrthoDB" id="1681849at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016646">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-NH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.110.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002563">
        <property type="entry name" value="Flavin_Rdtase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012349">
        <property type="entry name" value="Split_barrel_FMN-bd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01613">
        <property type="entry name" value="Flavin_Reduct"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00903">
        <property type="entry name" value="Flavin_Reduct"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Flavin_Reduct" evidence="1">
        <location>
            <begin position="15"/>
            <end position="167"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00903"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF09851.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="205" mass="21825" checksum="BE434215CADFA5A1" modified="2006-05-30" version="1">MASHRLDVDLAKAYRLLNHGPTVLVSAAHNGQRNIMAAAWAMPLDFAPPKVAVVLDKSTWTRQLLEASGEFALQVPTAAQVDITQALGSSSGRALTEKQGIDKFAAYGLQTFAGSVIGAPLLEGCAAWLECRLLPEPAIQNSYDLFLGEVVAAHADGRVFSNGRWHFTGQDDLRTIHHVAGGHFLVDGDAIDARPLTPPVTPPAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="99" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LJ52</accession>
    <name>Q1LJ52_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Histidine kinase</fullName>
            <ecNumber evidence="2">2.7.13.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">phcS</name>
        <name evidence="6" type="ordered locus">Rmet_2951</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.</text>
            <dbReference type="EC" id="2.7.13.3"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.7.13.3" evidence="2"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09824.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2951"/>
    <dbReference type="EnsemblBacteria" id="ABF09824">
        <property type="protein sequence ID" value="ABF09824"/>
        <property type="gene ID" value="Rmet_2951"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2951"/>
    <dbReference type="eggNOG" id="COG4191">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_619445_0_0_4"/>
    <dbReference type="OMA" id="MAVQATH"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00082">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003661">
        <property type="entry name" value="HisK_dim/P"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036097">
        <property type="entry name" value="HisK_dim/P_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004358">
        <property type="entry name" value="Sig_transdc_His_kin-like_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00512">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00344">
        <property type="entry name" value="BCTRLSENSOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00388">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47384">
        <property type="entry name" value="SSF47384"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="SSF55874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="39"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="70"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="96"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="128"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="153"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="176"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="5">
        <location>
            <begin position="247"/>
            <end position="462"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABF09824.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="462" mass="50524" checksum="8BA7F5A0754CE1E1" modified="2006-05-30" version="1">MSSPKPLSVEDSAAQPAKAMSSNKLYAGYQSELADFRLAFSRGGAYTAIVLVLLGVGLDYGQYPQWQVPFAAARIVVSLLIAGVVVALYSQAGRRFAPWLTMTWLLLPQIMIAWMISQTEGVESPYYVGLNLAIFASGIALPFGLWQNLVFGVLSYVLYAAACLLHPGGIEPVGTFIVNSLFLLFAAAASGVYTFFNERARFMLFRLKAEVADKNAELEVINRKLVDIKGQMLQQEKMAAIGTLAAGLLHEVNNPVNFCLMAIEVAMEEPAAKSEPGIQECLVDAKQGMQRIQHIVSDLKTFAYRKPGAEVEGTPFLFEKALDSSQRLTAHELRGVKMTREMPDDTLVLGDEAAIIGVLINLFSNAALAMRKAGTQNPAIHTTVRWEDKRLRVTVRDNGPGIAQEHLARVFEPFFTTREVGQGLGLGLSISYAVIERHGGVLYAESEVGQWAAFSFDLPRAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LJJ7</accession>
    <name>Q1LJJ7_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">DNA polymerase III chi subunit</fullName>
            <ecNumber evidence="1">2.7.7.7</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">holC</name>
        <name evidence="1" type="ordered locus">Rmet_2806</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.7.7.7" evidence="1"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09679.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517375.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2806"/>
    <dbReference type="EnsemblBacteria" id="ABF09679">
        <property type="protein sequence ID" value="ABF09679"/>
        <property type="gene ID" value="Rmet_2806"/>
    </dbReference>
    <dbReference type="GeneID" id="60820743"/>
    <dbReference type="KEGG" id="rme:Rmet_2806"/>
    <dbReference type="eggNOG" id="COG2927">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_131584_2_0_4"/>
    <dbReference type="OMA" id="WQFEPSA"/>
    <dbReference type="OrthoDB" id="1575254at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003887">
        <property type="term" value="F:DNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007459">
        <property type="entry name" value="DNA_pol3_chi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036768">
        <property type="entry name" value="PolIII_chi_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38767">
        <property type="entry name" value="PTHR38767"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04364">
        <property type="entry name" value="DNA_pol3_chi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102400">
        <property type="entry name" value="SSF102400"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF09679.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="144" mass="15811" checksum="29405D049912DC6E" modified="2006-05-30" version="1">MTRIDFHSNVPDTLTYVCRLVRKAYGAGQKVVVHGAPQQLAQLDARLWSFSPLDFLPHCGVDSPNAAVTPIILAAALDGVPHHQLLVNLAPEAPAQFASFERLIEVVGATPEARDSGRERYRFYRERGYPLTHHDIGQAKGDAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LJS6</accession>
    <name>Q1LJS6_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative glycosyl transferase, group 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_2727</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09600.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2727"/>
    <dbReference type="CAZy" id="GT4">
        <property type="family name" value="Glycosyltransferase Family 4"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF09600">
        <property type="protein sequence ID" value="ABF09600"/>
        <property type="gene ID" value="Rmet_2727"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2727"/>
    <dbReference type="eggNOG" id="COG0438">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009583_8_0_4"/>
    <dbReference type="OMA" id="SYYREGI"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001296">
        <property type="entry name" value="Glyco_trans_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028098">
        <property type="entry name" value="Glyco_trans_4-like_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13579">
        <property type="entry name" value="Glyco_trans_4_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00534">
        <property type="entry name" value="Glycos_transf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glyco_trans_4-like_N" evidence="2">
        <location>
            <begin position="49"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="domain" description="Glycos_transf_1" evidence="1">
        <location>
            <begin position="217"/>
            <end position="373"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13579"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF09600.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="403" mass="44573" checksum="533C79DBD22CC69E" modified="2006-05-30" version="1">MKSDLEECHEHRGNSEVQQLHEMAPKILISTTVPETFATILRGQPGYLASRFQVTLATSPADMLAKVETAEGLRVLSVPMARGINPLGDIKSIVRMIQVLREVRPQLVHSYTPKAGLVTMLAAWLCRVPVRVHTFTGLIFPTAHGIKQRILIWVDRLICFCSTRIVPEGLGVKRDLEKFGITRKPLCVIGSGNIAGVDTSYFSPALSGIAAGASDLKVKLAIEDRDFVFCFVGRLNRDKGVAELVSAFSVLPKNAILILVGAIDETAPVDENTLEMIASDQRIHHLGFMDDIRPALMLADVLVLPSYREGFPNVILQAGSMELPVIATDINGCNEVIEDGFNGWLVPPRDAVSLGRVMKSAMESPLSVLREMGVRARARIHERFERSQHWDRMIEFYSKLLNT</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-10-07" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LJY8</accession>
    <name>Q1LJY8_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_2665</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09538.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2665"/>
    <dbReference type="EnsemblBacteria" id="ABF09538">
        <property type="protein sequence ID" value="ABF09538"/>
        <property type="gene ID" value="Rmet_2665"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2665"/>
    <dbReference type="HOGENOM" id="CLU_2234304_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="83"/>
            <end position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF09538.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="105" mass="12332" checksum="BE1B556F769C8359" modified="2006-05-30" version="1">MIMHTYQYELVDPKCRSSINLAEHAKDIAHAFYFVQFQISDLRFYRDRFKFSVQSPLTPADARSMGRHIASCAKKLAQAATRVYNQKKSQDPKFAQSKQLFKRRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LKF9</accession>
    <name>Q1LKF9_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Putative citrate lyase beta subunit (CitE)</fullName>
            <ecNumber evidence="6">4.1.3.6</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Rmet_2490</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <dbReference type="EC" id="4.1.3.6" evidence="6"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09367.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517069.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2490"/>
    <dbReference type="EnsemblBacteria" id="ABF09367">
        <property type="protein sequence ID" value="ABF09367"/>
        <property type="gene ID" value="Rmet_2490"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2490"/>
    <dbReference type="eggNOG" id="COG2301">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_073837_0_0_4"/>
    <dbReference type="OMA" id="VCDHYAG"/>
    <dbReference type="OrthoDB" id="1107373at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008815">
        <property type="term" value="F:citrate (pro-3S)-lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005000">
        <property type="entry name" value="Aldolase/citrate-lyase_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040186">
        <property type="entry name" value="Citramalyl-CoA_lyase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011206">
        <property type="entry name" value="Citrate_lyase_beta/mcl1/mcl2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015813">
        <property type="entry name" value="Pyrv/PenolPyrv_Kinase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040442">
        <property type="entry name" value="Pyrv_Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11105">
        <property type="entry name" value="PTHR11105"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03328">
        <property type="entry name" value="HpcH_HpaI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015582">
        <property type="entry name" value="Cit_lyase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51621">
        <property type="entry name" value="SSF51621"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0456">Lyase</keyword>
    <keyword evidence="4" id="KW-0460">Magnesium</keyword>
    <keyword evidence="2 4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HpcH_HpaI" evidence="5">
        <location>
            <begin position="46"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="83"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3 4">
        <location>
            <position position="148"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="174"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR015582-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR015582-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF03328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABF09367.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="326" mass="36095" checksum="EE4743A81222AEA5" modified="2006-05-30" version="1">MHPSEVLFQGEAIPVQLPVCDHYAGSEKLMRKSLALQQALGPVFDITFDCEDGAAVGLEREHATLCAQLINSAENAHNRVGVRIHDPAHPCWREDVDILVRTAGARLAYVVVPKVTDVVEVARVTDHVNQVARASGIARHIPIHVLIETHSALEQVFDIASLVQVECLSFGLMDFVSAHHGAIPGEAMRSPEQFEHPLIRRAMLEISAACHRHGKVPSHNVSTDVQNPQRSGDDAYRARAEFGYLRKWSIHPGQIEPIVSAFRPGDSEIGAASQILLAAHENNWAPIRHDGQLHDRASYRYYWSLLQRAHATGTKLPSPVTELFFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LKG4</accession>
    <name>Q1LKG4_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Succinate dehydrogenase hydrophobic membrane anchor subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">sdhD</name>
        <name evidence="16" type="ordered locus">Rmet_2485</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Membrane-anchoring subunit of succinate dehydrogenase (SDH).</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="14">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
        <text evidence="14">The heme is bound between the two transmembrane subunits.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3">Carbohydrate metabolism; tricarboxylic acid cycle.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Part of an enzyme complex containing four subunits: a flavoprotein, an iron-sulfur protein, plus two membrane-anchoring proteins, SdhC and SdhD.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09362.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008649354.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2485"/>
    <dbReference type="EnsemblBacteria" id="ABF09362">
        <property type="protein sequence ID" value="ABF09362"/>
        <property type="gene ID" value="Rmet_2485"/>
    </dbReference>
    <dbReference type="GeneID" id="60821040"/>
    <dbReference type="KEGG" id="rme:Rmet_2485"/>
    <dbReference type="eggNOG" id="COG2142">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_151315_2_1_4"/>
    <dbReference type="OMA" id="QWMKVLT"/>
    <dbReference type="OrthoDB" id="2048140at2"/>
    <dbReference type="UniPathway" id="UPA00223"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000104">
        <property type="term" value="F:succinate dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1300.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034804">
        <property type="entry name" value="SQR/QFR_C/D"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014312">
        <property type="entry name" value="Succ_DH_anchor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000701">
        <property type="entry name" value="SuccDH_FuR_B_TM-su"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38689">
        <property type="entry name" value="PTHR38689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01127">
        <property type="entry name" value="Sdh_cyt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000169">
        <property type="entry name" value="SDH_D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81343">
        <property type="entry name" value="SSF81343"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02968">
        <property type="entry name" value="succ_dehyd_anc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7 14" id="KW-0349">Heme</keyword>
    <keyword evidence="11 14" id="KW-0408">Iron</keyword>
    <keyword evidence="12 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="9 14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 15" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="27"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="65"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="94"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="14">
        <location>
            <position position="77"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
            <note>ligand shared with second transmembrane subunit</note>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="89"/>
        </location>
        <ligand>
            <name>a ubiquinone</name>
            <dbReference type="ChEBI" id="CHEBI:16389"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004050"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011558"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00019425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR000169-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR000169-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="ABF09362.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="121" mass="13668" checksum="DE6CA229C56C52DD" modified="2006-05-30" version="1">MANNNIGPKRLVVGAHYGLKDWLAQRVTAVIMVVFTVVLAAVYLAFGNPSYEGWSGLFANQWMKILTFLTILSLLFHAWIGVRDIWMDYVKPMAVRLVLQVLTILWLVGCAGYAAQILWRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LKP0</accession>
    <name>Q1LKP0_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Phosphatidylglycerophosphate synthetase</fullName>
            <ecNumber evidence="13">2.7.8.5</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">pgsA</name>
        <name evidence="13" type="ordered locus">Rmet_2409</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="10">Phospholipid metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the CDP-alcohol phosphatidyltransferase class-I family.</text>
    </comment>
    <dbReference type="EC" id="2.7.8.5" evidence="13"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09286.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517005.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2409"/>
    <dbReference type="DNASU" id="4039231"/>
    <dbReference type="EnsemblBacteria" id="ABF09286">
        <property type="protein sequence ID" value="ABF09286"/>
        <property type="gene ID" value="Rmet_2409"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2409"/>
    <dbReference type="eggNOG" id="COG0558">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_051314_2_1_4"/>
    <dbReference type="OMA" id="WSMVYYL"/>
    <dbReference type="OrthoDB" id="1702107at2"/>
    <dbReference type="UniPathway" id="UPA00035">
        <property type="reaction ID" value="UER00042"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008444">
        <property type="term" value="F:CDP-diacylglycerol-glycerol-3-phosphate 3-phosphatidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008654">
        <property type="term" value="P:phospholipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1760">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000462">
        <property type="entry name" value="CDP-OH_P_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043130">
        <property type="entry name" value="CDP-OH_PTrfase_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004570">
        <property type="entry name" value="Phosphatidylglycerol_P_synth"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01066">
        <property type="entry name" value="CDP-OH_P_transf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000847">
        <property type="entry name" value="Phos_ph_gly_syn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00560">
        <property type="entry name" value="pgsA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00379">
        <property type="entry name" value="CDP_ALCOHOL_P_TRANSF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="6" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="7 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword evidence="9" id="KW-1208">Phospholipid metabolism</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 11 13" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="38"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="78"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="124"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="158"/>
            <end position="178"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00025707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU003750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ABF09286.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="199" mass="22071" checksum="58AE37C017BCB905" modified="2006-05-30" version="1">MPLNIPILLTWLRVAMIPLVVGVYYLPEAWLPMHTKNVTAASFFVIAAVTDWLDGFLARRWNQTSAFGAFLDPVADKLMVTAALLSLLALGRVADLIALIIIGREITISALREWMAQIGASKSVAVNFLGKLKTTVQMIAIPLLLFNDALFGVDASLLGGWMIYLAAVLTLWSMFYYMKLAWPQIRERSEVISGARTQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LKR2</accession>
    <name>Q1LKR2_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 8">Heme exporter protein C</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Cytochrome c-type biogenesis protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8 10" type="primary">ccmC</name>
        <name evidence="10" type="ordered locus">Rmet_2387</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 8">Required for the export of heme to the periplasm for the biogenesis of c-type cytochromes.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cell inner membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 8">Belongs to the CcmC/CycZ/HelC family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09264.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_003149822.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2387"/>
    <dbReference type="EnsemblBacteria" id="ABF09264">
        <property type="protein sequence ID" value="ABF09264"/>
        <property type="gene ID" value="Rmet_2387"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2387"/>
    <dbReference type="eggNOG" id="COG0755">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_066538_2_0_4"/>
    <dbReference type="OMA" id="CGRLLPW"/>
    <dbReference type="OrthoDB" id="955123at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015232">
        <property type="term" value="F:heme transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017004">
        <property type="term" value="P:cytochrome complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002541">
        <property type="entry name" value="Cyt_c_assembly"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003557">
        <property type="entry name" value="Cyt_c_biogenesis_CcmC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045062">
        <property type="entry name" value="Cyt_c_biogenesis_CcsA/CcmC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30071">
        <property type="entry name" value="PTHR30071"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01578">
        <property type="entry name" value="Cytochrom_C_asm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01386">
        <property type="entry name" value="CCMCBIOGNSIS"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01191">
        <property type="entry name" value="ccmC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="8" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-0201">Cytochrome c-type biogenesis</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="8" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="20"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="62"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="90"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="128"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="160"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="199"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="domain" description="Cytochrom_C_asm" evidence="9">
        <location>
            <begin position="27"/>
            <end position="183"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002442"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00016463"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU364092"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01578"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ABF09264.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="245" mass="27162" checksum="69DA8C0721E116FF" modified="2006-05-30" version="1">MVNWFKFSSPKAFYPLAGKLVPWLGVTAAALSLAGVYVGFFLAPTDAQQGEGYRIIFLHVPVSWMSMVIYLAMAFWAGIGLVFNSRLSAMMASALAPTGALFTFLSLWTGALWGKPMWGTWWVWDARLTSELVLLFLYIGFIALQSAIEDRRRADRAGAVLALVGVVNIPVIYFSVQWWNTLHQGASVSLTRSPSMATVMLLGMLIMVLAAWAYTAAAALARVRCIILEREHHAGWLQDIEEVKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="54" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LKT7</accession>
    <name>Q1LKT7_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">RES domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_2362</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the MbcT/ParT/Res family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09239.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_003149772.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2362"/>
    <dbReference type="EnsemblBacteria" id="ABF09239">
        <property type="protein sequence ID" value="ABF09239"/>
        <property type="gene ID" value="Rmet_2362"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2362"/>
    <dbReference type="eggNOG" id="ENOG502Z82U">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_113762_0_0_4"/>
    <dbReference type="OMA" id="SRDYTHT"/>
    <dbReference type="OrthoDB" id="1840242at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014914">
        <property type="entry name" value="RES_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08808">
        <property type="entry name" value="RES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00953">
        <property type="entry name" value="RES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RES" evidence="2">
        <location>
            <begin position="28"/>
            <end position="175"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF09239.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="210" mass="23256" checksum="8264E187F84FA350" modified="2006-05-30" version="1">MSHELPSFLIDAGELLQHVSRVVYRGSPLYYGRSSTNRYDDPARAYGVLYLGRDLPTALMESVFHKHQWLADTKRSIALKEVQARMVRAVGVMDDVLLADLTAPGVMAGYFGLNLEQLASRDYTHTQQVSAQVHAILGDDGQALFDGVLYPSRNNYPAKSIALFERAAAKVGVVDDIDLVDHVDWPHFVATYRVDVEPDPGPVEPDDEAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="55" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LKT8</accession>
    <name>Q1LKT8_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_2361</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09238.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_003149770.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2361"/>
    <dbReference type="DNASU" id="4039181"/>
    <dbReference type="EnsemblBacteria" id="ABF09238">
        <property type="protein sequence ID" value="ABF09238"/>
        <property type="gene ID" value="Rmet_2361"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2361"/>
    <dbReference type="eggNOG" id="ENOG502ZBV0">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_133794_2_0_4"/>
    <dbReference type="OMA" id="RCEQQIS"/>
    <dbReference type="OrthoDB" id="2056606at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022213">
        <property type="entry name" value="DUF3742"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12553">
        <property type="entry name" value="DUF3742"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="31"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="57"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF09238.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="119" mass="13268" checksum="AC9F537CD592AEB5" modified="2006-05-30" version="1">MSTTTRISTAERLGRALGRGWRAYARSERRASNWLVSKGVPLAAAAALLWAGKLAVLGLLLYVAFWLALLLVALVFVARGHDTVEWEPPEPEWRHGHAGFGLYTHDEYRIDSHDPSDET</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LL25</accession>
    <name>Q1LL25_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">UPF0114 protein Rmet_2272</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Rmet_2272</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cell membrane</location>
            <topology evidence="6">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the UPF0114 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09151.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516977.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2272"/>
    <dbReference type="EnsemblBacteria" id="ABF09151">
        <property type="protein sequence ID" value="ABF09151"/>
        <property type="gene ID" value="Rmet_2272"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2272"/>
    <dbReference type="eggNOG" id="COG2862">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_097887_0_0_4"/>
    <dbReference type="OMA" id="HTIMWQV"/>
    <dbReference type="OrthoDB" id="1466526at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00143">
        <property type="entry name" value="UPF0114"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005134">
        <property type="entry name" value="UPF0114"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020761">
        <property type="entry name" value="UPF0114_bac"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38596">
        <property type="entry name" value="PTHR38596"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03350">
        <property type="entry name" value="UPF0114"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00645">
        <property type="entry name" value="HI0507"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="21"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="59"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="140"/>
            <end position="160"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005774"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00143"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABF09151.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="184" mass="20408" checksum="9F7446584ECB006E" modified="2006-05-30" version="1">MAHSPLRPIPRIIFASRWLQLPLYLGLIVAQGVYVYHFLVEVWHLLSHVTTFDETKIMLVVLGLIDVVMISNLLIMVIVGGYETFVSRLGIDNHPDEPEWLDHVNAGVLKVKLSMALIGISSIHLLKTFIDADQRSEHTIMWQVIIHLAFLLSAVAMAWVDRMVTHPHPAAGPGAAGGAAETTH</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="108" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LL59</accession>
    <name>Q1LL59_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Glycerol kinase</fullName>
            <ecNumber evidence="5">2.7.1.30</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">ATP:glycerol 3-phosphotransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="5">Glycerokinase</fullName>
            <shortName evidence="5">GK</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5 9" type="primary">glpK</name>
        <name evidence="9" type="ordered locus">Rmet_2238</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Key enzyme in the regulation of glycerol uptake and metabolism. Catalyzes the phosphorylation of glycerol to yield sn-glycerol 3-phosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>ATP + glycerol = ADP + H(+) + sn-glycerol 3-phosphate</text>
            <dbReference type="Rhea" id="RHEA:21644"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17754"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57597"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.30"/>
        </reaction>
    </comment>
    <comment type="activity regulation">
        <text evidence="5">Inhibited by fructose 1,6-bisphosphate (FBP).</text>
    </comment>
    <comment type="pathway">
        <text evidence="5">Polyol metabolism; glycerol degradation via glycerol kinase pathway; sn-glycerol 3-phosphate from glycerol: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 5 6">Belongs to the FGGY kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.30" evidence="5"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09117.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2238"/>
    <dbReference type="EnsemblBacteria" id="ABF09117">
        <property type="protein sequence ID" value="ABF09117"/>
        <property type="gene ID" value="Rmet_2238"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2238"/>
    <dbReference type="eggNOG" id="COG0554">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009281_2_3_4"/>
    <dbReference type="OMA" id="FMLMNIG"/>
    <dbReference type="UniPathway" id="UPA00618">
        <property type="reaction ID" value="UER00672"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004370">
        <property type="term" value="F:glycerol kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019563">
        <property type="term" value="P:glycerol catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006072">
        <property type="term" value="P:glycerol-3-phosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00186">
        <property type="entry name" value="Glycerol_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000577">
        <property type="entry name" value="Carb_kinase_FGGY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018485">
        <property type="entry name" value="Carb_kinase_FGGY_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018483">
        <property type="entry name" value="Carb_kinase_FGGY_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018484">
        <property type="entry name" value="Carb_kinase_FGGY_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005999">
        <property type="entry name" value="Glycerol_kin"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02782">
        <property type="entry name" value="FGGY_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00370">
        <property type="entry name" value="FGGY_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000538">
        <property type="entry name" value="GlpK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="SSF53067"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01311">
        <property type="entry name" value="glycerol_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00933">
        <property type="entry name" value="FGGY_KINASES_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00445">
        <property type="entry name" value="FGGY_KINASES_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 5" id="KW-0319">Glycerol metabolism</keyword>
    <keyword evidence="5 6 9" id="KW-0418">Kinase</keyword>
    <keyword evidence="2 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 6 9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="FGGY_N" evidence="7">
        <location>
            <begin position="11"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="domain" description="FGGY_C" evidence="8">
        <location>
            <begin position="266"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="5">
        <location>
            <begin position="89"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="5">
        <location>
            <begin position="250"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="19"/>
            <end position="21"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="19"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="23"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="141"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="272"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="315"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="319"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="416"/>
            <end position="420"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022798"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ABF09117.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="510" mass="55986" checksum="77FADD0B8D8F931F" modified="2006-05-30" version="1">MTSTTSHQPQYVLALDQGTSSSRAILFDHLGNVVRIAQREFRQYYPHPGHVEHDPYEIWQSQLAVAHEVLGDAGVSGTQISAIGITNQRETTVLWDRKTGEPVGRALVWQDRRTAPMCEDLQAAGHAETFQRKTGLIVDAYFSGTKLRWMLDNYEGARARAERGELAFGTVDSWLIWQLTDGARHVTDVSNASRTMLFNIHDFRWDDELLGLLDIPRNLLPEVVPSSGEVARASARLFGVEIPIAGIAGDQQAATFGQACLRPGMAKNTYGTGCFILMNTGDKPVTSHNRLITTIGWQIGGNTQYCLEGGVFMGGATIQWLRDGLKLIHSAPEVEPLARQCTDTGGVVLVPAFAGLGAPHWDPFARGTLVGITRGTGGPHIARAALESIALQSVDVLDAMQKDAGISLAELRVDGGASRSDLLMQMQADLLGTVVVRPRVTETTALGAAYLAGLATGYWTDPEEITRQWQVEQRFEPKLSADERERRIARWHRAVDRARDWARESDDAAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LL84</accession>
    <name>Q1LL84_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Cob(II)yrinic acid a,c-diamide reductase (BluB-like)</fullName>
            <ecNumber evidence="2">1.16.8.1</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_2213</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EC" id="1.16.8.1" evidence="2"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09092.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516920.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2213"/>
    <dbReference type="EnsemblBacteria" id="ABF09092">
        <property type="protein sequence ID" value="ABF09092"/>
        <property type="gene ID" value="Rmet_2213"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2213"/>
    <dbReference type="eggNOG" id="COG0778">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_070764_3_1_4"/>
    <dbReference type="OMA" id="FMQPWDF"/>
    <dbReference type="OrthoDB" id="1847197at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043784">
        <property type="term" value="F:cob(II)yrinic acid a,c-diamide reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.109.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012825">
        <property type="entry name" value="BluB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029479">
        <property type="entry name" value="Nitroreductase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000415">
        <property type="entry name" value="Nitroreductase-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00881">
        <property type="entry name" value="Nitroreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55469">
        <property type="entry name" value="SSF55469"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02476">
        <property type="entry name" value="BluB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nitroreductase" evidence="1">
        <location>
            <begin position="21"/>
            <end position="182"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF09092.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="227" mass="25149" checksum="6A91EC22615DB400" modified="2006-05-30" version="1">MSGPHVEASEREALYRIMALRRDCRHFTPGPLLADDQLERLLDAAHQAPSVGLMQPWRFMRLSTPAWREHLIPFVEAERQATAQALGERAAEFLRIKVEGIRDCAELLAVVLAPDDGTVFGRRSMPREMAWASAACAVQNLWLAARAENLGLGWVSMFDPEALARELQLPEGATPFGLLCLGPVPAFYEKPMLEQIGWRQRRPLQEMLWASPDLPPASGGGEQAGGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LLC0</accession>
    <name>Q1LLC0_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Exopolyphosphatase</fullName>
            <ecNumber evidence="3">3.6.1.11</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">ppx</name>
        <name evidence="6" type="ordered locus">Rmet_2177</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>[phosphate](n) + H2O = [phosphate](n-1) + H(+) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:21528"/>
            <dbReference type="Rhea" id="RHEA-COMP:9859"/>
            <dbReference type="Rhea" id="RHEA-COMP:14279"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16838"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="EC" id="3.6.1.11"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the GppA/Ppx family.</text>
    </comment>
    <dbReference type="EC" id="3.6.1.11" evidence="3"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09056.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516887.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2177"/>
    <dbReference type="EnsemblBacteria" id="ABF09056">
        <property type="protein sequence ID" value="ABF09056"/>
        <property type="gene ID" value="Rmet_2177"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2177"/>
    <dbReference type="eggNOG" id="COG0248">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025908_4_0_4"/>
    <dbReference type="OMA" id="RISEGCY"/>
    <dbReference type="OrthoDB" id="1862004at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004309">
        <property type="term" value="F:exopolyphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006793">
        <property type="term" value="P:phosphorus metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022371">
        <property type="entry name" value="Exopolyphosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003695">
        <property type="entry name" value="Ppx_GppA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030673">
        <property type="entry name" value="PyroPPase_GppA_Ppx"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02541">
        <property type="entry name" value="Ppx-GppA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001267">
        <property type="entry name" value="Pyrophosphatase_GppA_Ppx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="SSF53067"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03706">
        <property type="entry name" value="exo_poly_only"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ppx-GppA" evidence="5">
        <location>
            <begin position="41"/>
            <end position="315"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012451"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00020416"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF02541"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABF09056.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="510" mass="56387" checksum="13AA33D963131148" modified="2006-05-30" version="1">MTQTPRLLAAVDMGSNSFRLMIGRVDETPTASGPASQIFQVDALREPVRLAAGLTPDKYLDQPARRRGIEALRRFGDRLRDFSPDQVRAVATNTLRVAKNASEFLIEAEAALGFPIEVIAGREEARLIYLGASHDAPACQGNRLVVDIGGGSTEFIIGSGYQSKLMESLYIGCVSHSRQFFPSGNVDEYAMKQAELAARREIQVLVRQYRAAGWQQAVGSSGTARALAELIELNGMNDSASEHGITREGLERLKRALVKAENANRVKLAGLKADRIPVLPGGLSIMLGVFAELDLDRMDVTDGALRLGVLYDLLGRSHHEDMRTVTVDQFMRRYGVDRAQAGRVGVAARDLLSQFPDPPNERRSDNLALLGWAASLHEIGMTISHSGYHKHSAYIATHADMPGFSKTDQARLATLLLGHASKLSKLSGSGKFLDWRMLFSLRLAFVLCRRRADVKLPEIQVGQRSDELDEGFEVRLPKSWIDMNPLVEYSLAQEADEWERIGKRYKVVYV</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LLC5</accession>
    <name>Q1LLC5_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Tyr recombinase activity site-specific recombination Tyr recombinase activity</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">int</name>
        <name evidence="3" type="ordered locus">Rmet_2172</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09051.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516881.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF09051">
        <property type="protein sequence ID" value="ABF09051"/>
        <property type="gene ID" value="Rmet_2172"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2172"/>
    <dbReference type="eggNOG" id="COG0582">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_579853_0_0_4"/>
    <dbReference type="OMA" id="GCRTHEA"/>
    <dbReference type="OrthoDB" id="1776972at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.443.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011010">
        <property type="entry name" value="DNA_brk_join_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013762">
        <property type="entry name" value="Integrase-like_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002104">
        <property type="entry name" value="Integrase_catalytic"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00589">
        <property type="entry name" value="Phage_integrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56349">
        <property type="entry name" value="SSF56349"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51898">
        <property type="entry name" value="TYR_RECOMBINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tyr recombinase" evidence="2">
        <location>
            <begin position="206"/>
            <end position="426"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF09051.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="464" mass="51739" checksum="7E2DA5166BA98260" modified="2006-05-30" version="1">MYKHVSLRRRQLSGRTSWLLLGPDGRPIDAFTAFAESLRNAPANTRDSYCRHLAEFFDYLIEAAALIGQGRQLTKLELTETLEAYGDYLLLGIDASRPIAQAVASSLPPGVNQASSVVPKKAALRRFLKLSEDVRKEMAELARLYDSRGVIADTPLLPELGQKRPLQLGEVRAMQAHSMIAGVVAGGPKFVECVVLGDGAADAPYDEQRAFPYDKVMDLIDAMPTYRDKAFYALLAASGCRTHEALQVLLNDDIDVDEGTVRLVDPKSRSGHSSYRSLTPSQRELLAWKGRTSDLTLLIEPFATSFFDSLQGYLEQEHLAHGKHGFLFQYLKGLERGQPYFLSAPGTRLELFHRVQKHIGVMLPPGTGPHSLRHMYGTYVLNYFPRANGDYGLPVPMVQQLLGHADINSTLKYAKFDKDLFKLEVQNANRVLFSHGTPKKLMELKLEALRAQMAKVQSQLSQRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="107" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LLF0</accession>
    <name>Q1LLF0_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Enoyl-[acyl-carrier-protein] reductase [NADH]</fullName>
            <ecNumber evidence="6">1.3.1.9</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">fabI</name>
        <name evidence="10" type="ordered locus">Rmet_2147</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>a 2,3-saturated acyl-[ACP] + NAD(+) = a (2E)-enoyl-[ACP] + H(+) + NADH</text>
            <dbReference type="Rhea" id="RHEA:10240"/>
            <dbReference type="Rhea" id="RHEA-COMP:9925"/>
            <dbReference type="Rhea" id="RHEA-COMP:9926"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:78784"/>
            <dbReference type="ChEBI" id="CHEBI:78785"/>
            <dbReference type="EC" id="1.3.1.9"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Lipid metabolism; fatty acid biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the short-chain dehydrogenases/reductases (SDR) family. FabI subfamily.</text>
    </comment>
    <dbReference type="EC" id="1.3.1.9" evidence="6"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09026.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008641690.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2147"/>
    <dbReference type="EnsemblBacteria" id="ABF09026">
        <property type="protein sequence ID" value="ABF09026"/>
        <property type="gene ID" value="Rmet_2147"/>
    </dbReference>
    <dbReference type="GeneID" id="60821265"/>
    <dbReference type="KEGG" id="rme:Rmet_2147"/>
    <dbReference type="eggNOG" id="COG0623">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010194_10_1_4"/>
    <dbReference type="OMA" id="GILDMIH"/>
    <dbReference type="OrthoDB" id="762291at2"/>
    <dbReference type="UniPathway" id="UPA00094"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004318">
        <property type="term" value="F:enoyl-[acyl-carrier-protein] reductase (NADH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016631">
        <property type="term" value="F:enoyl-[acyl-carrier-protein] reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006633">
        <property type="term" value="P:fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd05372">
        <property type="entry name" value="ENR_SDR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014358">
        <property type="entry name" value="Enoyl-ACP_Rdtase_NADH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43159">
        <property type="entry name" value="PTHR43159"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000094">
        <property type="entry name" value="Enoyl-ACP_rdct"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="5 6" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="6" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="5 6" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="4 6 9" id="KW-0520">NAD</keyword>
    <keyword evidence="3 6 10" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Proton acceptor" evidence="7">
        <location>
            <position position="146"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="7">
        <location>
            <position position="156"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="13"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="19"/>
            <end position="20"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="64"/>
            <end position="65"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="92"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="95"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="163"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="192"/>
            <end position="196"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009233"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR000094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR000094-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR000094-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR000094-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ABF09026.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="260" mass="27817" checksum="7EFB610123DE264C" modified="2006-05-30" version="1">MGFLAGKRILITGLLSNRSIAYGIAAACKREGAELAFTYVGERFKDRITDFAKEFGSDMIYECDVGSDEQIDATFAALGQRWEKFDGLVHSIGFAPREAIAGNFLDGLSREGFRIAHDISAYSFPALAKAAMPLLNDKASLLTLTYLGAERVVPNYNTMGLAKASLEASVRYLASAVGPRGMRANGISAGPIKTLAASGIKDFSRLLKHFEDVAPIRRNVTIEEVGNVAAFLLSDLASGVTGEITYVDGGYNIVGTAVED</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="101" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LLK9</accession>
    <name>Q1LLK9_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Ribose-5-phosphate isomerase A</fullName>
            <ecNumber evidence="2">5.3.1.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">Phosphoriboisomerase A</fullName>
            <shortName evidence="2">PRI</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="3" type="primary">cbbI2</name>
        <name evidence="2" type="synonym">rpiA</name>
        <name evidence="3" type="ordered locus">Rmet_2088</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Catalyzes the reversible conversion of ribose-5-phosphate to ribulose 5-phosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>aldehydo-D-ribose 5-phosphate = D-ribulose 5-phosphate</text>
            <dbReference type="Rhea" id="RHEA:14657"/>
            <dbReference type="ChEBI" id="CHEBI:58121"/>
            <dbReference type="ChEBI" id="CHEBI:58273"/>
            <dbReference type="EC" id="5.3.1.6"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Carbohydrate degradation; pentose phosphate pathway; D-ribose 5-phosphate from D-ribulose 5-phosphate (non-oxidative stage): step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ribose 5-phosphate isomerase family.</text>
    </comment>
    <dbReference type="EC" id="5.3.1.6" evidence="2"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08967.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516801.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2088"/>
    <dbReference type="EnsemblBacteria" id="ABF08967">
        <property type="protein sequence ID" value="ABF08967"/>
        <property type="gene ID" value="Rmet_2088"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2088"/>
    <dbReference type="eggNOG" id="COG0120">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_056590_1_1_4"/>
    <dbReference type="OMA" id="YDWDEVN"/>
    <dbReference type="OrthoDB" id="1681738at2"/>
    <dbReference type="UniPathway" id="UPA00115">
        <property type="reaction ID" value="UER00412"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004751">
        <property type="term" value="F:ribose-5-phosphate isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009052">
        <property type="term" value="P:pentose-phosphate shunt, non-oxidative branch"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01398">
        <property type="entry name" value="RPI_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00170">
        <property type="entry name" value="Rib_5P_isom_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037171">
        <property type="entry name" value="NagB/RpiA_transferase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020672">
        <property type="entry name" value="Ribose5P_isomerase_typA_subgr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004788">
        <property type="entry name" value="Ribose5P_isomerase_type_A"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11934">
        <property type="entry name" value="PTHR11934"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06026">
        <property type="entry name" value="Rib_5-P_isom_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100950">
        <property type="entry name" value="SSF100950"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00021">
        <property type="entry name" value="rpiA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 2 3" id="KW-0413">Isomerase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Substrate binding" evidence="2">
        <location>
            <begin position="32"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="2">
        <location>
            <begin position="85"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="2">
        <location>
            <begin position="98"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="2">
        <location>
            <position position="107"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="125"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF08967.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="228" mass="23854" checksum="62BC3C2DA0AE43F0" modified="2006-05-30" version="1">MTQDELKALVAQAAADYVKQEVPEGAVLGVGTGSTANLFIDAVAAFKERFSGAVSSSEASTRRLQQHGFNVLDLNEVDSIPVYVDGADEIDNSGAMIKGGGGALTREKIVASVADRFVCIADGSKLVPTMGAFPLPVEVIPMARAAVARSLAALGGQPRLRMTKEGGIYKTDNGNVILDVSGLKIENPKALEQQINQLPGVVTVGLFALRGANVLLLGTEQGVQRSDY</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="96" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LLW3</accession>
    <name>Q1LLW3_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Aminotransferase</fullName>
            <ecNumber evidence="1">2.6.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">asdA</name>
        <name evidence="3" type="ordered locus">Rmet_1984</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.6.1.-" evidence="1"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08863.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516704.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1984"/>
    <dbReference type="EnsemblBacteria" id="ABF08863">
        <property type="protein sequence ID" value="ABF08863"/>
        <property type="gene ID" value="Rmet_1984"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1984"/>
    <dbReference type="eggNOG" id="COG0436">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038911_0_0_4"/>
    <dbReference type="OMA" id="IFTPYIE"/>
    <dbReference type="OrthoDB" id="554560at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008483">
        <property type="term" value="F:transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901605">
        <property type="term" value="P:alpha-amino acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009058">
        <property type="term" value="P:biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022518">
        <property type="entry name" value="Aspartate_4-decarboxylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004838">
        <property type="entry name" value="NHTrfase_class1_PyrdxlP-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="SSF53383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03801">
        <property type="entry name" value="asp_4_decarbox"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00105">
        <property type="entry name" value="AA_TRANSFER_CLASS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="3" id="KW-0456">Lyase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aminotran_1_2" evidence="2">
        <location>
            <begin position="190"/>
            <end position="507"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU000481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF08863.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="538" mass="60465" checksum="588B22754D3C8874" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="100" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LM96</accession>
    <name>Q1LM96_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Short-chain dehydrogenase/reductase SDR</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_1851</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08730.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516578.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1851"/>
    <dbReference type="EnsemblBacteria" id="ABF08730">
        <property type="protein sequence ID" value="ABF08730"/>
        <property type="gene ID" value="Rmet_1851"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1851"/>
    <dbReference type="eggNOG" id="COG1028">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010194_1_1_4"/>
    <dbReference type="OMA" id="VYLCCKA"/>
    <dbReference type="OrthoDB" id="1294334at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00061">
        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF08730.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="260" mass="27421" checksum="245CD6D479066D3F" modified="2006-05-30" version="1">MRTIQQLFDLKGRTALITGGSRGLGLQIAEALGEQGARVVLSARKADELQTAQEHLKSRGIAAEWIAADGSRDAEIARLADEALAKLGHVDILVNNAGATWGAPAEDHPVEAWDKVMNLNIRGLFLLTQRIGKLSMIPRRSGRVINVASIAGLAGNPPGSMETIAYNTSKGAVVNFTRTLAGEWGEHNITVNALAPGFFPSKMTQGSLDRMGVDKMCEGVPLHRLGDDDDLKGAALLFASDAGKHITGQILAVDGGVSAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LMA9</accession>
    <name>Q1LMA9_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">MFS transporter, ACS family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Rmet_1838</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08717.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516568.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1838"/>
    <dbReference type="EnsemblBacteria" id="ABF08717">
        <property type="protein sequence ID" value="ABF08717"/>
        <property type="gene ID" value="Rmet_1838"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1838"/>
    <dbReference type="eggNOG" id="COG2271">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001265_0_0_4"/>
    <dbReference type="OMA" id="VIVCWFN"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="SSF103473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="31"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="69"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="100"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="125"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="159"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="194"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="269"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="303"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="335"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="360"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="386"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="426"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="domain" description="MFS" evidence="6">
        <location>
            <begin position="35"/>
            <end position="450"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABF08717.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="452" mass="49627" checksum="C5CDCBCB7677D923" modified="2006-05-30" version="1">MTASNPAVGTPVADTRLDADGTIEKRAYNKVFWRIMPFLMLCYVIAYLDRVNVGFAKLQMAQDLQFSETVFGLGAGLFFIGYFLFEVPSNLLMHHIGARIWIARIMITWGIISAAFVFVQTPTQFYVMRFLLGLAEAGFYPGVILYLTYWYPSHRRAKMIALFMSGIPVAGMFGNPLSGWIMDAFHNTHGWTGWQWMFLIEALPAFAIGIATIFVLRDGIDRAPWLSPDEKHVLKRNIEDDERAASAAATTGGQRHVHSLGAVFRDSRVWWMCLIYFCFVTGQYALTFWMPTLVKASGVTGNFNIGLLSAIPFLCAIVVMNILGHSADARRERRWHLIVPALMGATGFAIAASFTHNTTVAIAALSLAAAGVLTCAPLFWSLPTAFLSGIAAASGIAVVNSVGNLAGFVSPYMVGALKDMTQSTQLPMYVLSAILVVGALLVWLTPAKLVNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LMD6</accession>
    <name>Q1LMD6_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative hydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">ymdC</name>
        <name evidence="3" type="ordered locus">Rmet_1811</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08690.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516540.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1811"/>
    <dbReference type="EnsemblBacteria" id="ABF08690">
        <property type="protein sequence ID" value="ABF08690"/>
        <property type="gene ID" value="Rmet_1811"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1811"/>
    <dbReference type="eggNOG" id="COG1502">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026287_0_0_4"/>
    <dbReference type="OMA" id="DVSKCAP"/>
    <dbReference type="OrthoDB" id="1881748at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019637">
        <property type="term" value="P:organophosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025202">
        <property type="entry name" value="PLD-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001736">
        <property type="entry name" value="PLipase_D/transphosphatidylase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13091">
        <property type="entry name" value="PLDc_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00155">
        <property type="entry name" value="PLDc"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50035">
        <property type="entry name" value="PLD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="domain" description="PLD phosphodiesterase" evidence="2">
        <location>
            <begin position="170"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="domain" description="PLD phosphodiesterase" evidence="2">
        <location>
            <begin position="418"/>
            <end position="445"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF08690.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="532" mass="58317" checksum="4A1F1689BF1730E1" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="101" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LMF8</accession>
    <name>Q1LMF8_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Transcriptional regulator, GntR-family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Rmet_1789</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08668.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516518.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1789"/>
    <dbReference type="EnsemblBacteria" id="ABF08668">
        <property type="protein sequence ID" value="ABF08668"/>
        <property type="gene ID" value="Rmet_1789"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1789"/>
    <dbReference type="eggNOG" id="COG2186">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017584_9_1_4"/>
    <dbReference type="OMA" id="HNPYYRQ"/>
    <dbReference type="OrthoDB" id="1875458at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07377">
        <property type="entry name" value="WHTH_GntR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.530">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011711">
        <property type="entry name" value="GntR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008920">
        <property type="entry name" value="TF_FadR/GntR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000524">
        <property type="entry name" value="Tscrpt_reg_HTH_GntR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07729">
        <property type="entry name" value="FCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00392">
        <property type="entry name" value="GntR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00895">
        <property type="entry name" value="FCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00345">
        <property type="entry name" value="HTH_GNTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48008">
        <property type="entry name" value="SSF48008"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50949">
        <property type="entry name" value="HTH_GNTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH gntR-type" evidence="4">
        <location>
            <begin position="6"/>
            <end position="74"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABF08668.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="232" mass="25375" checksum="58FA0D8CA2D0A7BD" modified="2006-05-30" version="1">MQTRPITLTERVIQQLRGEIADGTFPVGARLPTGKQLAERYGVSAAVIREVTEHLRSLGMVESRQGLGSTVRSRVSDSGFRLSGGADNTDLADIFELRLDLESAAAAMAALHRTEQDIARMGALLDALAAQLDDADHGVEPDIQFHIAIATATHNKYYLQLLQYMTLQLHQAVRTARLNSSRQPGLPEQVHAEHVAIFDAIRARDAAQARLHAAAHLRNAAARLGLPMRQPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="101" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LMR4</accession>
    <name>Q1LMR4_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Phospho-2-dehydro-3-deoxyheptonate aldolase</fullName>
            <ecNumber evidence="5">2.5.1.54</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">aroG</name>
        <name evidence="8" type="ordered locus">Rmet_1681</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2 5">Stereospecific condensation of phosphoenolpyruvate (PEP) and D-erythrose-4-phosphate (E4P) giving rise to 3-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 5">
            <text>D-erythrose 4-phosphate + H2O + phosphoenolpyruvate = 7-phospho-2-dehydro-3-deoxy-D-arabino-heptonate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:14717"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16897"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58394"/>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
            <dbReference type="EC" id="2.5.1.54"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3 5">Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 1/7.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 5">Belongs to the class-I DAHP synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.54" evidence="5"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08562.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516410.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1681"/>
    <dbReference type="EnsemblBacteria" id="ABF08562">
        <property type="protein sequence ID" value="ABF08562"/>
        <property type="gene ID" value="Rmet_1681"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1681"/>
    <dbReference type="eggNOG" id="COG0722">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030903_0_1_4"/>
    <dbReference type="OMA" id="DLHIVHR"/>
    <dbReference type="OrthoDB" id="853329at2"/>
    <dbReference type="UniPathway" id="UPA00053">
        <property type="reaction ID" value="UER00084"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003849">
        <property type="term" value="F:3-deoxy-7-phosphoheptulonate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009073">
        <property type="term" value="P:aromatic amino acid family biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008652">
        <property type="term" value="P:cellular amino acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009423">
        <property type="term" value="P:chorismate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006218">
        <property type="entry name" value="DAHP1/KDSA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006219">
        <property type="entry name" value="DHAP_synth_1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21225">
        <property type="entry name" value="PTHR21225"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00793">
        <property type="entry name" value="DAHP_synth_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001361">
        <property type="entry name" value="DAHP_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00034">
        <property type="entry name" value="aroFGH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="5" id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="DAHP_synth_1" evidence="7">
        <location>
            <begin position="65"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00003726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007985"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRNR" id="PIRNR001361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ABF08562.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="380" mass="41585" checksum="4EB554D1970D8207" modified="2006-05-30" version="1">MNDPQKSPPSVAEGWQAPPDRTSVTDDARVEDIIPLPPPEHLIRFFPIRGTPVESLVTQTRQRISRILHGSDDRLLVIMGPCSIHDPQAALDYARRLAAERERYADSLEIVMRVYFEKPRTTVGWKGLINDPYLDESYRIDEGLRIARSLLVDINRLGLPAAGEFLDVISPQYIGDLICWGAIGARTTESQVHRELASGVSAPIGFKNGTDGNIKIAIDAIQAASRPHHFLGVHKNGQVATVHTKGNPDCHVILRGGKAPNYDAEVVAAACKELEAARLRNSLMVDCSHANSNKQHQRQIDVARDVAQQISGGSQSIFGLMVESHLVPGAQKFTPGEHNPSGLTYGQSITDACIGWEDSVTVLELLSEAVNVRRGVNRKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LN41</accession>
    <name>Q1LN41_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative conjugal transfer transmembrane protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">trbD</name>
        <name evidence="2" type="ordered locus">Rmet_1552</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08435.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516289.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1552"/>
    <dbReference type="EnsemblBacteria" id="ABF08435">
        <property type="protein sequence ID" value="ABF08435"/>
        <property type="gene ID" value="Rmet_1552"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1552"/>
    <dbReference type="eggNOG" id="COG5268">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_189159_0_0_4"/>
    <dbReference type="OMA" id="VWATKRD"/>
    <dbReference type="OrthoDB" id="2047516at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016704">
        <property type="entry name" value="Conjugal_tfr_TrbD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007792">
        <property type="entry name" value="T4SS_VirB3/TrbD/AvhB"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05101">
        <property type="entry name" value="VirB3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017854">
        <property type="entry name" value="T4SS_TrbD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="49"/>
            <end position="68"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF08435.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="90" mass="9688" checksum="FE9073E4DC6BC309" modified="2006-05-30" version="1">MSAPDTFADGFEVPLHRSLTEPILLGGAPRTVAIANGTLTAAVGLGLQLWIPGVVLWIVGHSLAVWGARVDPQFMQVFARHIKHRPLLDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="106" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LN79</accession>
    <name>Q1LN79_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Phosphoglycolate phosphatase (PGP)</fullName>
            <ecNumber evidence="6">3.1.3.18</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">cbbZ1</name>
        <name evidence="6" type="ordered locus">Rmet_1514</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <dbReference type="EC" id="3.1.3.18" evidence="6"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08397.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516257.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1514"/>
    <dbReference type="EnsemblBacteria" id="ABF08397">
        <property type="protein sequence ID" value="ABF08397"/>
        <property type="gene ID" value="Rmet_1514"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1514"/>
    <dbReference type="eggNOG" id="COG0546">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045011_19_1_4"/>
    <dbReference type="OMA" id="WAVPYGY"/>
    <dbReference type="OrthoDB" id="1562270at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008967">
        <property type="term" value="F:phosphoglycolate phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.240">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006439">
        <property type="entry name" value="HAD-SF_hydro_IA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041492">
        <property type="entry name" value="HAD_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023198">
        <property type="entry name" value="PGP-like_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006346">
        <property type="entry name" value="PGPase/MupP"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13419">
        <property type="entry name" value="HAD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00413">
        <property type="entry name" value="HADHALOGNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="SSF56784"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01549">
        <property type="entry name" value="HAD-SF-IA-v1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01509">
        <property type="entry name" value="HAD-SF-IA-v3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01449">
        <property type="entry name" value="PGP_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="3 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0460">Magnesium</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABF08397.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="243" mass="26054" checksum="45C1D56DA05F8128" modified="2006-05-30" version="1">MRYDIVSFDLDGTLVDTAAEIAEAANRALESHGIARRPVSEVTVLIGAGTRELMLKLLARVMIEQPHLADRVHPDQVLASMDEHYAVTTGTSSVPYPGALEALSALKAAGIKLACVTNKEFRHAERVLRVHRLDAYFDLVVGGDSLRVKKPDPGVLRHVVERLGGSTDRTGHVGDSRVDVEAARNAGVTAWAVPYGYNAGQPIEDAYPERLFPSLADLAQHVLAGRSGAPCVSLTSTQPTISS</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="62" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LNA8</accession>
    <name>Q1LNA8_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Replication initiation protein CMGI-3 (GI family Tn4371)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">repA</name>
        <name evidence="1" type="ordered locus">Rmet_1485</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08368.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1485"/>
    <dbReference type="EnsemblBacteria" id="ABF08368">
        <property type="protein sequence ID" value="ABF08368"/>
        <property type="gene ID" value="Rmet_1485"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1485"/>
    <dbReference type="eggNOG" id="COG5534">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_050846_0_0_4"/>
    <dbReference type="OMA" id="AISHIMR"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018777">
        <property type="entry name" value="Replication_initiator_prot_A"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10134">
        <property type="entry name" value="RPA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF08368.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="289" mass="32785" checksum="0596637EC025B7EC" modified="2006-05-30" version="1">MAFAMSSPALPSRQRPLQEREQLDLFRALPGDMAPRDSQDLMAYPFFSLGKSKRVKPIDFRAGNVTIRVEGTQEHGIATIWDADVLIWAASQIVEAKDAGLRPSRLMRATPYEILRFIGRGKSLRDYQRLKAALDRLQSTTVATSIRETTGRRLHRFSWINEWKELADASGTPLGIELILPDWFYAGVLDAALVLTIDPAYFRLTGGIERWLYRLVRKHGGKQAYGWQFDFRYLHQKSGSTAKPYEFACDLRALVARQSLPGYVLGIERMPDSGMELLTFRPVPHTARG</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LNC0</accession>
    <name>Q1LNC0_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative RNA-directed DNA polymerase (Reverse transcriptase)</fullName>
            <ecNumber evidence="2">2.7.7.49</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_1473</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.7.7.49" evidence="2"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08356.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516221.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1473"/>
    <dbReference type="EnsemblBacteria" id="ABF08356">
        <property type="protein sequence ID" value="ABF08356"/>
        <property type="gene ID" value="Rmet_1473"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1473"/>
    <dbReference type="eggNOG" id="COG3344">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028398_1_0_4"/>
    <dbReference type="OMA" id="RDYILCA"/>
    <dbReference type="OrthoDB" id="1581397at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003964">
        <property type="term" value="F:RNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03487">
        <property type="entry name" value="RT_Bac_retron_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000123">
        <property type="entry name" value="Reverse_transcriptase_msDNA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000477">
        <property type="entry name" value="RT_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00078">
        <property type="entry name" value="RVT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00866">
        <property type="entry name" value="RNADNAPOLMS"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="SSF56672"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50878">
        <property type="entry name" value="RT_POL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0695">RNA-directed DNA polymerase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Reverse transcriptase" evidence="1">
        <location>
            <begin position="31"/>
            <end position="247"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF08356.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="323" mass="35789" checksum="25815C89F7138BEF" modified="2006-05-30" version="1">MSQSATNAPSYSQRRIGTLQALHVALGMPVGDLPTLAERANDLYRVAKSITKPDGSIRNTYDAREPLKEVHRRIKSQILDHVNYPAYLTGSIKGCDYKVNASLHARARIVINEDISGFFPSTSADRVFSIWRGFFGFSEDVARCLTQLTTRHGELPQGAITSSFLANLVFWWDEPELFGKFAAQGLVYSRYVDDIAVSSKTFLTNEAKTNVVRQVYGMLLKHGYKAKRAKHEIATSGSRMAVTKLAVNNKPGLDKATRNKIRAVVHAVERRIAQGEALSFERGPYAQAMGQVLHLERFHPGDAAPLKRRLLALKEAGAALPHS</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LNX5</accession>
    <name>Q1LNX5_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative RNA Modifying Enzyme</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_1266</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08151.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516037.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1266"/>
    <dbReference type="EnsemblBacteria" id="ABF08151">
        <property type="protein sequence ID" value="ABF08151"/>
        <property type="gene ID" value="Rmet_1266"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1266"/>
    <dbReference type="eggNOG" id="COG1236">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009673_5_2_4"/>
    <dbReference type="OMA" id="FSCHAGM"/>
    <dbReference type="OrthoDB" id="580619at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022712">
        <property type="entry name" value="Beta_Casp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001279">
        <property type="entry name" value="Metallo-B-lactamas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011108">
        <property type="entry name" value="RMMBL"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10996">
        <property type="entry name" value="Beta-Casp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00753">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07521">
        <property type="entry name" value="RMMBL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01027">
        <property type="entry name" value="Beta-Casp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00849">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="SSF56281"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Lactamase_B" evidence="1">
        <location>
            <begin position="13"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="domain" description="Beta-Casp" evidence="2">
        <location>
            <begin position="252"/>
            <end position="377"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM01027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF08151.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="469" mass="52082" checksum="EB1B6B4F094D2997" modified="2006-05-30" version="1">MKLSFLGAAREVTGSCFLVEAANVRFLVDCGMVQGGRVAAARNYEPFAFDPASIDFVLLTHAHIDHSGLLPKLTRAGFKGPIYATPATVDLLGVMLPDSAHIQESDAKRVAKRFKEKTVPPPLYTLQDTHECLQQVRGIEYDREFAPRVGVRARFRDAGHILGSAIVEVWITEYGYPTKIVFSGDLGQPGRPILRDPTPIEDADILVIESTYGDRRHKDFSATEADMIGIVEKTLFERGGNVIVPAFAVGRTQEVLYHLHRLTCEGRLQRPMVFVDSPMATEATRITREHLELFDEQAKRLAGWHARGENLPYLDFTASAEESMALNRIRSGAIIISASGMCDAGRIRHHLRHNLPRRECSILFPGFQAQGTLGRRLIEGAERVRIFGEDIPVRAAIHSVDGLSAHADQKALLDWARAFIQAPAQTFVVHGESSAAQAFAELLQQQLGWQVTVPEYGHALRWPDFLAHE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-12-12" modified="2022-08-03" version="101" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LP64</accession>
    <name>ENO2_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Enolase 2</fullName>
            <ecNumber evidence="1">4.2.1.11</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">2-phospho-D-glycerate hydro-lyase 2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">2-phosphoglycerate dehydratase 2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">eno2</name>
        <name type="ordered locus">Rmet_1176</name>
    </gene>
    <organism>
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the reversible conversion of 2-phosphoglycerate into phosphoenolpyruvate. It is essential for the degradation of carbohydrates via glycolysis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(2R)-2-phosphoglycerate = H2O + phosphoenolpyruvate</text>
            <dbReference type="Rhea" id="RHEA:10164"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:58289"/>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
            <dbReference type="EC" id="4.2.1.11"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="activity regulation">
        <text evidence="1">The covalent binding to the substrate causes inactivation of the enzyme, and possibly serves as a signal for the export of the protein.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 4/5.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cell surface</location>
        </subcellularLocation>
        <text evidence="1">Fractions of enolase are present in both the cytoplasm and on the cell surface. The export of enolase possibly depends on the covalent binding to the substrate; once secreted, it remains attached to the cell surface.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the enolase family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.11" evidence="1"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08062.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515956.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q1LP64"/>
    <dbReference type="SMR" id="Q1LP64"/>
    <dbReference type="STRING" id="266264.Rmet_1176"/>
    <dbReference type="PRIDE" id="Q1LP64"/>
    <dbReference type="EnsemblBacteria" id="ABF08062">
        <property type="protein sequence ID" value="ABF08062"/>
        <property type="gene ID" value="Rmet_1176"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1176"/>
    <dbReference type="eggNOG" id="COG0148">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031223_2_1_4"/>
    <dbReference type="OMA" id="QHLGGAF"/>
    <dbReference type="OrthoDB" id="533698at2"/>
    <dbReference type="UniPathway" id="UPA00109">
        <property type="reaction ID" value="UER00187"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009986">
        <property type="term" value="C:cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000015">
        <property type="term" value="C:phosphopyruvate hydratase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004634">
        <property type="term" value="F:phosphopyruvate hydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006096">
        <property type="term" value="P:glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd03313">
        <property type="entry name" value="enolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.390.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00318">
        <property type="entry name" value="Enolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000941">
        <property type="entry name" value="Enolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036849">
        <property type="entry name" value="Enolase-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029017">
        <property type="entry name" value="Enolase-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020810">
        <property type="entry name" value="Enolase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020811">
        <property type="entry name" value="Enolase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11902">
        <property type="entry name" value="PTHR11902"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00113">
        <property type="entry name" value="Enolase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03952">
        <property type="entry name" value="Enolase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001400">
        <property type="entry name" value="Enolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00148">
        <property type="entry name" value="ENOLASE"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00002">
        <property type="entry name" value="enolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01192">
        <property type="entry name" value="Enolase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01193">
        <property type="entry name" value="Enolase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51604">
        <property type="entry name" value="SSF51604"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54826">
        <property type="entry name" value="SSF54826"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01060">
        <property type="entry name" value="eno"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0324">Glycolysis</keyword>
    <keyword id="KW-0456">Lyase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0964">Secreted</keyword>
    <feature type="chain" id="PRO_0000267085" description="Enolase 2">
        <location>
            <begin position="1"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="1">
        <location>
            <position position="205"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="1">
        <location>
            <position position="337"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="155"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="164"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="242"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="285"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="285"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="312"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="312"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" description="covalent; in inhibited form" evidence="1">
        <location>
            <position position="337"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="364"/>
            <end position="367"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="388"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00318"/>
        </source>
    </evidence>
    <sequence length="425" mass="45532" checksum="9752442518E0F051" modified="2006-05-30" version="1">MSKVAEIRGLEVLDSRGNPTVEVEVVLDDGAVGRAIVPSGASTGAREAVELRDADPRRYLGRGVLRAVQAVNTELCEALLGRDAGDQPEIDTIMIDLDGTPDKRRLGANALLGVSLAVAHAAAMSNGLPLFAYLGGERASLLPVPLINVINGGAHADNALDFQEFMIVPAGAPTFTEAVRASAEVFHTLRAMLKAAGYTTNVGDEGGFAPEFHAAEEALDILVAAIAKAGYRPGEDIALAIDPAASELYVNGSYVYQGEGVERSREEQVAYLVRLADRYPIVSIEDGMAEDDAMGWQLLTRQLGKRCQLVGDDVFCTHPTLLRQGVEQGMANAILVKANQIGTLSEMRETVRVAHGYAYSAVMSHRSGETEDVTIADLAVALRCGQIKTGSMSRADRTAKYNRLLRIERELGSRAEYAGALLRRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-12-02" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LPC2</accession>
    <name>Q1LPC2_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Ferric alcaligin E</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">aleB</name>
        <name evidence="10" type="ordered locus">Rmet_1118</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cell outer membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the TonB-dependent receptor family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08004.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1118"/>
    <dbReference type="EnsemblBacteria" id="ABF08004">
        <property type="protein sequence ID" value="ABF08004"/>
        <property type="gene ID" value="Rmet_1118"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1118"/>
    <dbReference type="eggNOG" id="COG4771">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015930_0_0_4"/>
    <dbReference type="OMA" id="MTGYRIP"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009279">
        <property type="term" value="C:cell outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015344">
        <property type="term" value="F:siderophore uptake transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038023">
        <property type="term" value="F:signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.130.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.170.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039426">
        <property type="entry name" value="BtuB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012910">
        <property type="entry name" value="Plug_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037066">
        <property type="entry name" value="Plug_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011662">
        <property type="entry name" value="Secretin/TonB_short_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000531">
        <property type="entry name" value="TonB-dep_rcpt_b-brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036942">
        <property type="entry name" value="TonB_rcpt_b-brl_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010105">
        <property type="entry name" value="TonB_sidphr_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30069">
        <property type="entry name" value="PTHR30069"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07715">
        <property type="entry name" value="Plug"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07660">
        <property type="entry name" value="STN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00593">
        <property type="entry name" value="TonB_dep_Rec"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00965">
        <property type="entry name" value="STN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01783">
        <property type="entry name" value="TonB-siderophor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0998">Cell outer membrane</keyword>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0675">Receptor</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="4 8" id="KW-0798">TonB box</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="STN" evidence="9">
        <location>
            <begin position="72"/>
            <end position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009810"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003357"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00965"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ABF08004.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="839" mass="91157" checksum="B88C46025458F202" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LPN4</accession>
    <name>Q1LPN4_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Phospholipase A1</fullName>
            <ecNumber evidence="13">3.1.1.32</ecNumber>
            <ecNumber evidence="13">3.1.1.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Phosphatidylcholine 1-acylhydrolase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ordered locus">Rmet_1006</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Hydrolysis of phosphatidylcholine with phospholipase A2 (EC 3.1.1.4) and phospholipase A1 (EC 3.1.1.32) activities.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2 13">
            <text>a 1,2-diacyl-sn-glycero-3-phosphocholine + H2O = a 1-acyl-sn-glycero-3-phosphocholine + a fatty acid + H(+)</text>
            <dbReference type="Rhea" id="RHEA:15801"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:28868"/>
            <dbReference type="ChEBI" id="CHEBI:57643"/>
            <dbReference type="ChEBI" id="CHEBI:58168"/>
            <dbReference type="EC" id="3.1.1.4"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 13">
            <text>a 1,2-diacyl-sn-glycero-3-phosphocholine + H2O = a 2-acyl-sn-glycero-3-phosphocholine + a fatty acid + H(+)</text>
            <dbReference type="Rhea" id="RHEA:18689"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:28868"/>
            <dbReference type="ChEBI" id="CHEBI:57643"/>
            <dbReference type="ChEBI" id="CHEBI:57875"/>
            <dbReference type="EC" id="3.1.1.32"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="13">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
        <text evidence="13">Binds 1 Ca(2+) ion per monomer. In the dimeric form the Ca(2+) is bound by different amino acids with binding of each Ca(2+) shared with ligands coming from each monomer. The Ca(2+) ion may have a role in catalysis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5 13">Homodimer; dimerization is reversible, and the dimeric form is the active one.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="13">Cell outer membrane</location>
            <topology evidence="13">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <text evidence="13">One of the very few enzymes located there.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 13">Belongs to the phospholipase A1 family.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.32" evidence="13"/>
    <dbReference type="EC" id="3.1.1.4" evidence="13"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07892.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1006"/>
    <dbReference type="EnsemblBacteria" id="ABF07892">
        <property type="protein sequence ID" value="ABF07892"/>
        <property type="gene ID" value="Rmet_1006"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1006"/>
    <dbReference type="eggNOG" id="COG2829">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_051993_0_0_4"/>
    <dbReference type="OMA" id="YTQFSLW"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009279">
        <property type="term" value="C:cell outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052740">
        <property type="term" value="F:1-acyl-2-lysophosphatidylserine acylhydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052739">
        <property type="term" value="F:phosphatidylserine 1-acylhydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008970">
        <property type="term" value="F:phospholipase A1 activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004623">
        <property type="term" value="F:phospholipase A2 activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00541">
        <property type="entry name" value="OMPLA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003187">
        <property type="entry name" value="PLipase_A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036541">
        <property type="entry name" value="PLipase_A1_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40457">
        <property type="entry name" value="PTHR40457"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02253">
        <property type="entry name" value="PLA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01486">
        <property type="entry name" value="PHPHLIPASEA1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56931">
        <property type="entry name" value="SSF56931"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-0106">Calcium</keyword>
    <keyword evidence="12 13" id="KW-0998">Cell outer membrane</keyword>
    <keyword evidence="10 13 14" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="13" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="13" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0732">Signal</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1134">Transmembrane beta strand</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011702"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU366027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ABF07892.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="469" mass="51793" checksum="8AA77FE8C8CF67D1" modified="2006-05-30" version="1">MPHAPHMVRLRASQLLSRPRHRSAVDIPPVRRLLLPMPRYVSGRVALPRTAFRLLLPTLASSAVLFHATPADAAVSVLQPPRVIDATQPLTLTLLITGDRDMRRYAVPDSLEVSASGDLVAPAPMTLKRVGKGPTVLNLRQGEHQTVSFRGEWPTTLRGVVRLDISGIDAAPVLITLNQAPGANPATVTAPVVAGTPAEPKVAVAPVSAGQVTPVVSAENVPASTPAPADLRDAQRLSFNEPMYIMFGAHDGANAKFQLSFKFRIYEGKDPASKRFIDNLYFAYTQFSLWDLHSASKPFEDTNYRPSLYYYLSDTGVKNSVISRLSIASGFEHESNGRAGADSRSINTLFLKPTMYFGDQNDWHWRVAPKVYAYLEKSENEDIAHYRGYMDLNLAYGKPDSWEFAATLRKGTRQMYGSVDAQASYPLSRLIPGTAGYLMLGYFYGYGESLLNYNQKTPWQIRLGYALSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-12-02" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LQ82</accession>
    <name>Q1LQ82_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Tautomerase</fullName>
            <ecNumber evidence="3">5.3.2.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Rmet_0808</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the 4-oxalocrotonate tautomerase family.</text>
    </comment>
    <dbReference type="EC" id="5.3.2.-" evidence="3"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07694.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0808"/>
    <dbReference type="EnsemblBacteria" id="ABF07694">
        <property type="protein sequence ID" value="ABF07694"/>
        <property type="gene ID" value="Rmet_0808"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0808"/>
    <dbReference type="eggNOG" id="COG1942">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_148073_5_3_4"/>
    <dbReference type="OMA" id="MHPGRTD"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016853">
        <property type="term" value="F:isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006725">
        <property type="term" value="P:cellular aromatic compound metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.429.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004370">
        <property type="entry name" value="4-oxalocrotonate_tautomerase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014347">
        <property type="entry name" value="Tautomerase/MIF_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018191">
        <property type="entry name" value="Tautomerase_Pseudo-typ"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01361">
        <property type="entry name" value="Tautomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55331">
        <property type="entry name" value="SSF55331"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00013">
        <property type="entry name" value="taut"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0413">Isomerase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tautomerase" evidence="4">
        <location>
            <begin position="3"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor; via imino nitrogen" evidence="2">
        <location>
            <position position="2"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR618191-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU362032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABF07694.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="62" mass="7061" checksum="DECB0A1CA769E0D8" modified="2006-05-30" version="1">MPTFHVEMFEGRTVEQKRKFVEEVTRVTCETLGCSSGAVDIIITDVKRENWATGGELWADKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="67" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LQG6</accession>
    <name>Q1LQG6_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">LapA_dom domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Rmet_0724</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07610.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008643660.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0724"/>
    <dbReference type="EnsemblBacteria" id="ABF07610">
        <property type="protein sequence ID" value="ABF07610"/>
        <property type="gene ID" value="Rmet_0724"/>
    </dbReference>
    <dbReference type="GeneID" id="60825211"/>
    <dbReference type="KEGG" id="rme:Rmet_0724"/>
    <dbReference type="eggNOG" id="COG5416">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_160072_1_0_4"/>
    <dbReference type="OMA" id="QQDATVH"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005887">
        <property type="term" value="C:integral component of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010445">
        <property type="entry name" value="LapA_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06305">
        <property type="entry name" value="LapA_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="37"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="domain" description="LapA_dom" evidence="6">
        <location>
            <begin position="23"/>
            <end position="82"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF06305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABF07610.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="107" mass="11428" checksum="F549C9959A0F7289" modified="2006-05-30" version="1">MKLVAWIFRIVLFVLLFVLALRNTGDASLQLFFGAVWHAPLILILLAAFALGIVAALAAVAPGLMRQRMEAGRLRRALGEARATSPAEAVPPEAKPGVPYNVVGPKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LQT1</accession>
    <name>Q1LQT1_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Acetyl/propionyl-CoA carboxylase,carboxyltransferase subunit</fullName>
            <ecNumber evidence="4">6.4.1.3</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">accB</name>
        <name evidence="4" type="ordered locus">Rmet_0609</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EC" id="6.4.1.3" evidence="4"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07495.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515468.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0609"/>
    <dbReference type="EnsemblBacteria" id="ABF07495">
        <property type="protein sequence ID" value="ABF07495"/>
        <property type="gene ID" value="Rmet_0609"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0609"/>
    <dbReference type="eggNOG" id="COG4799">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018822_0_1_4"/>
    <dbReference type="OMA" id="AYLPIMS"/>
    <dbReference type="OrthoDB" id="123223at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004658">
        <property type="term" value="F:propionyl-CoA carboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034733">
        <property type="entry name" value="AcCoA_carboxyl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029045">
        <property type="entry name" value="ClpP/crotonase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011763">
        <property type="entry name" value="COA_CT_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011762">
        <property type="entry name" value="COA_CT_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045190">
        <property type="entry name" value="MCCB/AccD1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22855">
        <property type="entry name" value="PTHR22855"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01039">
        <property type="entry name" value="Carboxyl_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52096">
        <property type="entry name" value="SSF52096"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50989">
        <property type="entry name" value="COA_CT_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50980">
        <property type="entry name" value="COA_CT_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0436">Ligase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="CoA carboxyltransferase N-terminal" evidence="2">
        <location>
            <begin position="22"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="domain" description="CoA carboxyltransferase C-terminal" evidence="3">
        <location>
            <begin position="275"/>
            <end position="527"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF07495.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="535" mass="58141" checksum="72164FC850F908E0" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="101" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LQY6</accession>
    <name>Q1LQY6_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">NAD-dependent formate dehydrogenase beta subunit</fullName>
            <ecNumber evidence="7">1.2.1.2</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">fdsB</name>
        <name evidence="7" type="ordered locus">Rmet_0554</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the complex I 51 kDa subunit family.</text>
    </comment>
    <dbReference type="EC" id="1.2.1.2" evidence="7"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07440.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515416.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0554"/>
    <dbReference type="EnsemblBacteria" id="ABF07440">
        <property type="protein sequence ID" value="ABF07440"/>
        <property type="gene ID" value="Rmet_0554"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0554"/>
    <dbReference type="eggNOG" id="COG1894">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014881_3_0_4"/>
    <dbReference type="OMA" id="DRMLMEG"/>
    <dbReference type="OrthoDB" id="688908at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008137">
        <property type="term" value="F:NADH dehydrogenase (ubiquinone) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1440.230">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11540">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001949">
        <property type="entry name" value="NADH-UbQ_OxRdtase_51kDa_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011538">
        <property type="entry name" value="Nuo51_FMN-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037225">
        <property type="entry name" value="Nuo51_FMN-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019575">
        <property type="entry name" value="Nuop51_4Fe4S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037207">
        <property type="entry name" value="Nuop51_4Fe4S-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01512">
        <property type="entry name" value="Complex1_51K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10589">
        <property type="entry name" value="NADH_4Fe-4S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00928">
        <property type="entry name" value="NADH_4Fe-4S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140490">
        <property type="entry name" value="SSF140490"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF142019">
        <property type="entry name" value="SSF142019"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="SSF52833"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00644">
        <property type="entry name" value="COMPLEX1_51K_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00645">
        <property type="entry name" value="COMPLEX1_51K_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="4" id="KW-0408">Iron</keyword>
    <keyword evidence="5" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NADH_4Fe-4S" evidence="6">
        <location>
            <begin position="426"/>
            <end position="471"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007523"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABF07440.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="518" mass="55099" checksum="92990D8CF834CF18" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="55" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LRB8</accession>
    <name>Q1LRB8_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_0422</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07308.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515297.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0422"/>
    <dbReference type="EnsemblBacteria" id="ABF07308">
        <property type="protein sequence ID" value="ABF07308"/>
        <property type="gene ID" value="Rmet_0422"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0422"/>
    <dbReference type="HOGENOM" id="CLU_111114_2_0_4"/>
    <dbReference type="OMA" id="NGNLAQM"/>
    <dbReference type="OrthoDB" id="1504440at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193782" evidence="1">
        <location>
            <begin position="31"/>
            <end position="203"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF07308.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="203" mass="21034" checksum="2F71F372AA09DC0A" modified="2006-05-30" version="1">MNLRAKPTSLISVLAAAAALAIGATGVAHAQKPQAKTPAPIGVAEEAVVSGKVVDVDPQSKAVLVQGPRGNLVELVAGDEAKNIGNVHKGDIVTLTRGAAVVAALQPVDSKDTPLLEQVERVSHAAEGGKPGVMREITTTITAQITKVDVAKRMVTFRGPRETLRTVKVQDPNIDLASIKQGQMAKIVVREVVAITVKSPNAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="62" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LRV2</accession>
    <name>Q1LRV2_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Ubiquinone biosynthesis accessory factor UbiK</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">ubiK</name>
        <name evidence="2" type="ordered locus">Rmet_0238</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Required for efficient ubiquinone (coenzyme Q) biosynthesis. UbiK is probably an accessory factor of Ubi enzymes and facilitates ubiquinone biosynthesis by acting as an assembly factor, a targeting factor, or both.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; ubiquinone biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the UbiK family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07124.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008652257.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0238"/>
    <dbReference type="EnsemblBacteria" id="ABF07124">
        <property type="protein sequence ID" value="ABF07124"/>
        <property type="gene ID" value="Rmet_0238"/>
    </dbReference>
    <dbReference type="GeneID" id="60822963"/>
    <dbReference type="KEGG" id="rme:Rmet_0238"/>
    <dbReference type="eggNOG" id="COG2960">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_154412_3_1_4"/>
    <dbReference type="OMA" id="KQPNDVF"/>
    <dbReference type="OrthoDB" id="1995361at2"/>
    <dbReference type="UniPathway" id="UPA00232"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006744">
        <property type="term" value="P:ubiquinone biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02216">
        <property type="entry name" value="UbiK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007475">
        <property type="entry name" value="UbiK"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38040">
        <property type="entry name" value="PTHR38040"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04380">
        <property type="entry name" value="BMFP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0831">Ubiquinone biosynthesis</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="49"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02216"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF07124.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="85" mass="9533" checksum="6AB3F701C47DBE45" modified="2006-05-30" version="1">MKPTDLFNDLQSKVSEALRNSPAKDIEKNVRSMMTQGFARLDLVTREEFDVQSQVLARTRARLEELEARVAELERRVGGTPTGGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="69" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LRY7</accession>
    <name>Q1LRY7_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Lipid A deacylase</fullName>
            <ecNumber evidence="2">3.1.1.77</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">LPS 3-O-deacylase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="2">Outer membrane enzyme</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Rmet_0203</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Has lipid A 3-O-deacylase activity. Hydrolyzes the ester bond at the 3 position of lipid A, a bioactive component of lipopolysaccharide (LPS), thereby releasing the primary fatty acyl moiety.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>a 3-(acyloxy)acyl derivative of bacterial toxin + H2O = 3-hydroxyacyl derivative of bacterial toxin + a fatty acid + H(+)</text>
            <dbReference type="Rhea" id="RHEA:12032"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:28868"/>
            <dbReference type="ChEBI" id="CHEBI:136853"/>
            <dbReference type="ChEBI" id="CHEBI:140675"/>
            <dbReference type="EC" id="3.1.1.77"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="2">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell outer membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PagL family.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.77" evidence="2"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07089.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0203"/>
    <dbReference type="EnsemblBacteria" id="ABF07089">
        <property type="protein sequence ID" value="ABF07089"/>
        <property type="gene ID" value="Rmet_0203"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0203"/>
    <dbReference type="eggNOG" id="COG3637">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_093405_0_0_4"/>
    <dbReference type="OMA" id="AYTYWDG"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009279">
        <property type="term" value="C:cell outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050528">
        <property type="term" value="F:acyloxyacyl hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018550">
        <property type="entry name" value="Lipid-A_deacylase-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011250">
        <property type="entry name" value="OMP/PagP_b-brl"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09411">
        <property type="entry name" value="PagL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF029681">
        <property type="entry name" value="PagL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56925">
        <property type="entry name" value="SSF56925"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 2" id="KW-0998">Cell outer membrane</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="site" description="Critical for activity" evidence="3">
        <location>
            <position position="205"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRNR" id="PIRNR029681"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR029681-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF07089.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="226" mass="24802" checksum="A9F8B26D7A75DE28" modified="2006-05-30" version="1">MAAYAVPFDVRCRANLSSMSMNKKTEQQEHHMPPANLSRKLPSARLLAIAALVAGASSAASAEELVGWAHPAVQAAFARDTDHGINKYEIAVNFNTPIQYGNPDGWLFRLQAEANMGYWDARSGTNRQNLMEFGLTPILRVEKRGGYFVPFLEAGVGLRLLTHTSTSDQHNFSTAFQFGDMVGLGVGFGKNAATEVGMRFQHISNAGIKEPNPGTNLYTGYVRYRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LS07</accession>
    <name>Q1LS07_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Dihydroneopterin aldolase</fullName>
            <ecNumber evidence="2">4.1.2.25</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">folB</name>
        <name evidence="2" type="ordered locus">Rmet_0183</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EC" id="4.1.2.25" evidence="2"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07069.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008641837.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0183"/>
    <dbReference type="EnsemblBacteria" id="ABF07069">
        <property type="protein sequence ID" value="ABF07069"/>
        <property type="gene ID" value="Rmet_0183"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0183"/>
    <dbReference type="eggNOG" id="COG1539">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_112632_0_1_4"/>
    <dbReference type="OMA" id="INIGVHD"/>
    <dbReference type="OrthoDB" id="1858654at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004150">
        <property type="term" value="F:dihydroneopterin aldolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006760">
        <property type="term" value="P:folic acid-containing compound metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1130.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006157">
        <property type="entry name" value="FolB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043133">
        <property type="entry name" value="GTP-CH-I_C/QueF"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02152">
        <property type="entry name" value="FolB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00905">
        <property type="entry name" value="FolB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00526">
        <property type="entry name" value="folB_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0456">Lyase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FolB" evidence="1">
        <location>
            <begin position="17"/>
            <end position="128"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00905"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF07069.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="132" mass="15122" checksum="4052318FF81B90C8" modified="2006-05-30" version="1">MTFAALSHPSLQNCRRMFLRNYEVQINIGVHEFEKKGEQRVLVNIDLFVSLAETTPQADKLEEVVDYDFMRNTVAERMARGHIHLQETLCDDVARAMLQHPKVRAVRVSTEKPDVYPDCDAVGVEVFHVKPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="96" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LSG6</accession>
    <name>Q1LSG6_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Carboxypeptidase G2 (CPDG2)</fullName>
            <ecNumber evidence="5">3.4.17.11</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Rmet_0024</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EC" id="3.4.17.11" evidence="5"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF06910.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011514957.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0024"/>
    <dbReference type="EnsemblBacteria" id="ABF06910">
        <property type="protein sequence ID" value="ABF06910"/>
        <property type="gene ID" value="Rmet_0024"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0024"/>
    <dbReference type="eggNOG" id="COG0624">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021802_7_2_4"/>
    <dbReference type="OMA" id="PMVHAEF"/>
    <dbReference type="OrthoDB" id="1015417at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004180">
        <property type="term" value="F:carboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001261">
        <property type="entry name" value="ArgE/DapE_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036264">
        <property type="entry name" value="Bact_exopeptidase_dim_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017150">
        <property type="entry name" value="Pept_M20_glutamate_carboxypep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002933">
        <property type="entry name" value="Peptidase_M20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011650">
        <property type="entry name" value="Peptidase_M20_dimer"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07687">
        <property type="entry name" value="M20_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01546">
        <property type="entry name" value="Peptidase_M20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037238">
        <property type="entry name" value="Carboxypeptidase_G2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55031">
        <property type="entry name" value="SSF55031"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00758">
        <property type="entry name" value="ARGE_DAPE_CPG2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00759">
        <property type="entry name" value="ARGE_DAPE_CPG2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0121">Carboxypeptidase</keyword>
    <keyword evidence="1 5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0645">Protease</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193416" evidence="3">
        <location>
            <begin position="37"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="domain" description="M20_dimer" evidence="4">
        <location>
            <begin position="230"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="active site" evidence="2">
        <location>
            <position position="132"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="2">
        <location>
            <position position="193"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR037238-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF07687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABF06910.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="438" mass="47134" checksum="E4D40AE6555393CE" modified="2006-05-30" version="1">MHAAPHPALRTRLARLALLPAALALAGAICALPAQAEPVQPAFQLAQQEKPALIDTLKDLVSIESGSKDIEGLDRIANLLRDRLAALGGDAKLVEPTEIYRMEDTPAKVGKMVMAQFKGTGQRKIMLIAHMDTVYLRGMLAQQPFRVDGDRAYGLGIADDKNGVAVILHTVAILQKMKFKDYGTLTVLINGDEEISSPGARATLTKLGAEQDAVFSCEASRVTGDRLSLATSGIGAISLKVDGKSSHAGSSPELGRNALYELSHQILQMRDLSNPDTGLKVNWTLSHAGTNRNVIPAVATAQADVRVLRTADYDSLERTLQERIQKKLIPDTKVEVKFERRRPPLEATPASRALAVHAQGIYAEIGEKLAVYDTAEGGGTDAAFAAAATKAPVIERFGLRGFGAHSNDAEYVDLNSVTPRLYLLTRMIMDVSQDKTGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="69" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LSH8</accession>
    <name>Q1LSH8_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Isoprenylcysteine carboxyl methyltransferase (ICMTase)</fullName>
            <ecNumber evidence="6">2.1.1.100</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Rmet_0012</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.1.1.100" evidence="6"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF06898.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011514946.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0012"/>
    <dbReference type="EnsemblBacteria" id="ABF06898">
        <property type="protein sequence ID" value="ABF06898"/>
        <property type="gene ID" value="Rmet_0012"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0012"/>
    <dbReference type="eggNOG" id="COG2020">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_065200_1_3_4"/>
    <dbReference type="OMA" id="DRDNRWV"/>
    <dbReference type="OrthoDB" id="1734625at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004671">
        <property type="term" value="F:protein C-terminal S-isoprenylcysteine carboxyl O-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007269">
        <property type="entry name" value="ICMT_MeTrfase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04140">
        <property type="entry name" value="ICMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="7"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="40"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="71"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="102"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="132"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="156"/>
            <end position="182"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABF06898.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="219" mass="24000" checksum="8C361076D8A3153D" modified="2006-05-30" version="1">MRPTPKMAIITIAGALAYLALPVFGWGGITAYFADTARTLAVVATLVMAVIAIFSGGNISAGEREDRDNRWVLVAFSVLSLLAGFLPAYTDRLDVWSIDGNTIRWIGLFLFVAGGALRLWPVFVLGRRFSGLVAIQPGHTLVTTGIYSVIRNPSYVGFVISSLGWGLVFRSWVGVLLALLMLPPLVARIRAEEALLQSQFGVEYEQYCARTWRMLPGLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-04-26" modified="2022-08-03" version="96" xmlns="http://uniprot.org/uniprot">
    <accession>Q58AJ2</accession>
    <name>Q58AJ2_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Conserved protein Tn6049</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">tnmC</name>
        <name evidence="1" type="ordered locus">Rmet_0319</name>
        <name evidence="2" type="ordered locus">Rmet_1998</name>
        <name evidence="3" type="ordered locus">Rmet_2010</name>
        <name evidence="4" type="ordered locus">Rmet_2407</name>
        <name evidence="5" type="ordered locus">Rmet_2550</name>
        <name evidence="6" type="ordered locus">Rmet_2835</name>
        <name evidence="7" type="ordered locus">Rmet_3140</name>
        <name evidence="8" type="ordered locus">Rmet_3929</name>
        <name evidence="9" type="ordered locus">Rmet_4422</name>
        <name evidence="10" type="ordered locus">Rmet_5478</name>
        <name evidence="11" type="ordered locus">Rmet_5943</name>
        <name evidence="12" type="ordered locus">Rmet_6337</name>
    </gene>
    <organism evidence="1 13">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="8">
        <name>megaplasmid</name>
    </geneLocation>
    <geneLocation type="plasmid" evidence="13">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <geneLocation type="plasmid" evidence="12 13">
        <name>pMOL28</name>
    </geneLocation>
    <geneLocation type="plasmid" evidence="11 13">
        <name>pMOL30</name>
    </geneLocation>
    <reference evidence="1" key="1">
        <citation type="submission" date="2006-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of the chromosome of Ralstonia metallidurans CH34.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Copeland A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Barry K."/>
                <person name="Detter J.C."/>
                <person name="Glavina del Rio T."/>
                <person name="Hammon N."/>
                <person name="Israni S."/>
                <person name="Dalin E."/>
                <person name="Tice H."/>
                <person name="Martinez M."/>
                <person name="Goltsman E."/>
                <person name="Pitluck S."/>
                <person name="Schmutz J."/>
                <person name="Larimer F."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Kim E."/>
                <person name="Mergeay M."/>
                <person name="Benotmane M.A."/>
                <person name="Vallaeys T."/>
                <person name="Michaux A."/>
                <person name="Monchy S."/>
                <person name="Dunn J."/>
                <person name="McCorkle S."/>
                <person name="Taghavi S."/>
                <person name="van der Lelie D."/>
                <person name="Richardson P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1">CH34</strain>
            <plasmid evidence="8">megaplasmid</plasmid>
            <plasmid evidence="12">pMOL28</plasmid>
            <plasmid evidence="11">pMOL30</plasmid>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="13">Plasmid megaplasmid CH34</plasmid>
            <plasmid evidence="13">Plasmid pMOL28</plasmid>
            <plasmid evidence="13">Plasmid pMOL30</plasmid>
        </source>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2010-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Benotmane R."/>
                <person name="Lapidus A."/>
                <person name="McCorkle S."/>
                <person name="Monchy S."/>
                <person name="Taghavi S."/>
                <person name="van der Lelie N."/>
                <person name="Dunn J."/>
                <person name="Leys N."/>
                <person name="Mergeay M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">CH34</strain>
            <plasmid evidence="8">megaplasmid</plasmid>
            <plasmid evidence="12">pMOL28</plasmid>
            <plasmid evidence="11">pMOL30</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07205.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08877.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08889.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09284.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09427.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09708.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10012.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10797.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11287.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12337.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP000354">
        <property type="protein sequence ID" value="ABF12802.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP000355">
        <property type="protein sequence ID" value="ABF13196.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011229369.1">
        <property type="nucleotide sequence ID" value="NC_006466.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_145626.1">
        <property type="nucleotide sequence ID" value="NC_006466.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0319"/>
    <dbReference type="EnsemblBacteria" id="ABF07205">
        <property type="protein sequence ID" value="ABF07205"/>
        <property type="gene ID" value="Rmet_0319"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF08877">
        <property type="protein sequence ID" value="ABF08877"/>
        <property type="gene ID" value="Rmet_1998"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF08889">
        <property type="protein sequence ID" value="ABF08889"/>
        <property type="gene ID" value="Rmet_2010"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF09284">
        <property type="protein sequence ID" value="ABF09284"/>
        <property type="gene ID" value="Rmet_2407"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF09427">
        <property type="protein sequence ID" value="ABF09427"/>
        <property type="gene ID" value="Rmet_2550"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF09708">
        <property type="protein sequence ID" value="ABF09708"/>
        <property type="gene ID" value="Rmet_2835"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF10012">
        <property type="protein sequence ID" value="ABF10012"/>
        <property type="gene ID" value="Rmet_3140"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF10797">
        <property type="protein sequence ID" value="ABF10797"/>
        <property type="gene ID" value="Rmet_3929"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF11287">
        <property type="protein sequence ID" value="ABF11287"/>
        <property type="gene ID" value="Rmet_4422"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF12337">
        <property type="protein sequence ID" value="ABF12337"/>
        <property type="gene ID" value="Rmet_5478"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF12802">
        <property type="protein sequence ID" value="ABF12802"/>
        <property type="gene ID" value="Rmet_5943"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF13196">
        <property type="protein sequence ID" value="ABF13196"/>
        <property type="gene ID" value="Rmet_6337"/>
    </dbReference>
    <dbReference type="GeneID" id="60824630"/>
    <dbReference type="KEGG" id="rme:Rmet_0319"/>
    <dbReference type="KEGG" id="rme:Rmet_1998"/>
    <dbReference type="KEGG" id="rme:Rmet_2010"/>
    <dbReference type="KEGG" id="rme:Rmet_2407"/>
    <dbReference type="KEGG" id="rme:Rmet_2550"/>
    <dbReference type="KEGG" id="rme:Rmet_2835"/>
    <dbReference type="KEGG" id="rme:Rmet_3140"/>
    <dbReference type="KEGG" id="rme:Rmet_3929"/>
    <dbReference type="KEGG" id="rme:Rmet_4422"/>
    <dbReference type="KEGG" id="rme:Rmet_5478"/>
    <dbReference type="KEGG" id="rme:Rmet_5943"/>
    <dbReference type="KEGG" id="rme:Rmet_6337"/>
    <dbReference type="eggNOG" id="ENOG5033BFG">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_202665_0_0_4"/>
    <dbReference type="OMA" id="IDANWTP"/>
    <dbReference type="OrthoDB" id="1900286at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid pMOL28"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid pMOL30"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0614">Plasmid</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF07205.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF08877.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF08889.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF09284.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABF09427.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABF09708.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABF10012.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ABF10797.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ABF11287.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ABF12337.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ABF12802.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ABF13196.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="71" mass="8016" checksum="3A7C9F9D0738696E" modified="2005-04-26" version="1">MTFHQPSGIAQGLPLLIDANWTPAQAWAVIELLDDLRDRLHAHYQLALAQWLAEDRQEHPDGLGDLADNDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-01-04" modified="2022-05-25" version="69" xmlns="http://uniprot.org/uniprot">
    <accession>Q5NV11</accession>
    <name>Q5NV11_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_6244</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="1 2">
        <name>pMOL28</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid pMOL28</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000355">
        <property type="protein sequence ID" value="ABF13103.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011239940.1">
        <property type="nucleotide sequence ID" value="NC_007972.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_161679.1">
        <property type="nucleotide sequence ID" value="NC_006525.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF13103">
        <property type="protein sequence ID" value="ABF13103"/>
        <property type="gene ID" value="Rmet_6244"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6244"/>
    <dbReference type="HOGENOM" id="CLU_1659316_0_0_4"/>
    <dbReference type="OMA" id="CAVYIRQ"/>
    <dbReference type="OrthoDB" id="1839756at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid pMOL28"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF13103.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="159" mass="18449" checksum="536D1F46C5F74F75" modified="2005-01-04" version="1">MSYPRTIRIEVGPVPAEESCAQVGHEDYSERSRRECAVYIRQLQRIFDNPDPEDLRFVRCGFPHELGRYHEVVAVMTAEGAKRFDEAMLPMQWDHIARAELTWLELQGKWRATVMARPEALALVPDVFRSGQIPQFPNHPIAQWWAMGFTPVTTAYRLH</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-03-23" modified="2020-10-07" version="31" xmlns="http://uniprot.org/uniprot">
    <accession>D3DXT6</accession>
    <name>D3DXT6_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_6498</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ADC45106.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_6498"/>
    <dbReference type="EnsemblBacteria" id="ADC45106">
        <property type="protein sequence ID" value="ADC45106"/>
        <property type="gene ID" value="Rmet_6498"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6498"/>
    <dbReference type="HOGENOM" id="CLU_3188138_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADC45106.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="46" mass="5190" checksum="433851A935CE5FC4" modified="2010-03-23" version="1">MANDAPMSSAFTRACKMLEIDDLHFRDMRHEAACDPDTFSLGLWTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-03-23" modified="2021-06-02" version="40" xmlns="http://uniprot.org/uniprot">
    <accession>D3DY15</accession>
    <name>D3DY15_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_6584</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ADC45185.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517692.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_6584"/>
    <dbReference type="EnsemblBacteria" id="ADC45185">
        <property type="protein sequence ID" value="ADC45185"/>
        <property type="gene ID" value="Rmet_6584"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6584"/>
    <dbReference type="HOGENOM" id="CLU_2131432_0_0_4"/>
    <dbReference type="OrthoDB" id="2101734at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="91"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="59"/>
            <end position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADC45185.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="113" mass="12298" checksum="C6771AC6B1236566" modified="2010-03-23" version="1">MATQRPVGTRSTSRSTSKASTSTSTSTSTNNSTQAGAPGSQPRRETPRAVDLGADTTIWHTRGLQTNSRMRMPVMQRTPSRQLPPSPIPRLILWAVLIGLALVCFNAVKHMLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-03-23" modified="2020-10-07" version="32" xmlns="http://uniprot.org/uniprot">
    <accession>D3DY85</accession>
    <name>D3DY85_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_4246</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ADC45255.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4246"/>
    <dbReference type="EnsemblBacteria" id="ADC45255">
        <property type="protein sequence ID" value="ADC45255"/>
        <property type="gene ID" value="Rmet_4246"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4246"/>
    <dbReference type="HOGENOM" id="CLU_2772949_0_0_4"/>
    <dbReference type="OrthoDB" id="1667004at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADC45255.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="69" mass="8182" checksum="C8FBE38E4E1A3A97" modified="2010-03-23" version="1">MVSGAVAEWHPKLHCAYAGFRERPFSPDFSSFWPIHRRFALMRHLLRNSLYCKDLAARISAWREFTELT</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-03-23" modified="2021-06-02" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>D3DY87</accession>
    <name>D3DY87_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_6657</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ADC45257.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADC45257">
        <property type="protein sequence ID" value="ADC45257"/>
        <property type="gene ID" value="Rmet_6657"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6657"/>
    <dbReference type="HOGENOM" id="CLU_3295404_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="18"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="19"/>
            <end position="40"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADC45257.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="40" mass="4476" checksum="6A336F92F9025C47" modified="2010-03-23" version="1">MGAAQLWSFVLPPTSIEPKFKVERGRQKPKHPEADFGTGH</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-03-23" modified="2020-10-07" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>D3DYD8</accession>
    <name>D3DYD8_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_6729</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ADC45308.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADC45308">
        <property type="protein sequence ID" value="ADC45308"/>
        <property type="gene ID" value="Rmet_6729"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6729"/>
    <dbReference type="HOGENOM" id="CLU_3011052_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADC45308.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="56" mass="6375" checksum="BC4001A14FD144E1" modified="2010-03-23" version="1">MRECGVPARIHEMIPYDLGGTALRDWAPCPCCYRKMYEMQVEGVVLAELVAEGEWT</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-03-23" modified="2020-10-07" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>D3DYE5</accession>
    <name>D3DYE5_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_6736</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ADC45315.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_6736"/>
    <dbReference type="EnsemblBacteria" id="ADC45315">
        <property type="protein sequence ID" value="ADC45315"/>
        <property type="gene ID" value="Rmet_6736"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6736"/>
    <dbReference type="HOGENOM" id="CLU_2247856_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADC45315.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="104" mass="11390" checksum="D73D697B9C52C055" modified="2010-03-23" version="1">MAFSFESQLEPLVQGALRHHPIPKPNVLKQIHCSLLEDSSTDSRFDFLSGPRLNHDRVDAFEVQEVRQQQASRACTNDSYLSSQNLVSVPAPMGVSAAKIPGGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-03-23" modified="2020-10-07" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>D3DYJ1</accession>
    <name>D3DYJ1_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_6384</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="1 2">
        <name>pMOL30</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid pMOL30</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000354">
        <property type="protein sequence ID" value="ADC45361.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADC45361">
        <property type="protein sequence ID" value="ADC45361"/>
        <property type="gene ID" value="Rmet_6384"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6384"/>
    <dbReference type="HOGENOM" id="CLU_2737170_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid pMOL30"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADC45361.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="71" mass="8013" checksum="B85027FDE63CF6DC" modified="2010-03-23" version="1">MSGRAWRVPLDPEFRPIELAAEVARALFSQFAPNADEITFLFKQDVEFFTPEQIGLAWLVPIAMWTSKTGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-10-07" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LA79</accession>
    <name>Q1LA79_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_6088</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="1 2">
        <name>pMOL30</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid pMOL30</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000354">
        <property type="protein sequence ID" value="ABF12947.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF12947">
        <property type="protein sequence ID" value="ABF12947"/>
        <property type="gene ID" value="Rmet_6088"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6088"/>
    <dbReference type="HOGENOM" id="CLU_1915343_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid pMOL30"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF12947.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="132" mass="14482" checksum="E9FF7182407D1E6D" modified="2006-05-30" version="1">MHSVGPIRPTQHTRLHNMVNPIYPHTSLDAMQLITFKYANGFSMMAELLPKPRVAGATITQVDVEITSARWSNVVQHSLPTLVQAGTQALLDAQTDAAQSTTHIAEIRITGEEFLTKHDMLQISGNLPVTVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="69" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LB87</accession>
    <name>Q1LB87_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative capsule polysaccharide export inner-membrane protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">kpsE</name>
        <name evidence="2" type="ordered locus">Rmet_5730</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12589.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5730"/>
    <dbReference type="EnsemblBacteria" id="ABF12589">
        <property type="protein sequence ID" value="ABF12589"/>
        <property type="gene ID" value="Rmet_5730"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5730"/>
    <dbReference type="eggNOG" id="COG3524">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027864_2_0_4"/>
    <dbReference type="OMA" id="LYMAFIA"/>
    <dbReference type="OrthoDB" id="792453at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="339"/>
            <end position="363"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF12589.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="368" mass="40528" checksum="7396E24AC9404956" modified="2006-05-30" version="1">MKTLSGVATAARRSPLLAAFVLFSLISGLYWGVIASDRYVSEAHIYLQKTNAIQQSSADLISILAGSGGNRDLLLLRDHLLSIDMMKLLDKDLHLREHFSDRRRDLLSRLWSKDAPDERFQRYYLSRIEVTYDDYAQVLSITAQAYSPEMARAIAQMMVTEGEKYMNALDHKTAHDQVSYIEQQVSALGVRMVAARKAVLAYQDAHGLVSPGNAVESISAIASRLDAELSDLMTKRAALAGYLTPAAPDIVQLDSQIAAVKSQLQAERQRVAAKRGGGLNAVAEEQGRLEAEAALADDMYKTALVALETARVESTRKIKSVAVLQSANLPVYPLEPRRIYNILLSLLAAAMLTGVLHLLVAIIRDHRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="66" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LBB6</accession>
    <name>Q1LBB6_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_5701</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12560.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011520103.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5701"/>
    <dbReference type="EnsemblBacteria" id="ABF12560">
        <property type="protein sequence ID" value="ABF12560"/>
        <property type="gene ID" value="Rmet_5701"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5701"/>
    <dbReference type="eggNOG" id="ENOG502ZCI4">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_064459_0_0_4"/>
    <dbReference type="OMA" id="SAESTWR"/>
    <dbReference type="OrthoDB" id="662528at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193288" evidence="2">
        <location>
            <begin position="21"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="171"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="172"/>
            <end position="190"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF12560.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="357" mass="38319" checksum="F43EC694214DC3FA" modified="2006-05-30" version="1">MQVIRNLWWLLVLASSLASAQVVTSKGMATVKYDGSLFSSKASAEDKQRAFQTAEVNAIERYYAESGESQAENFDSIRDKVAANLDKFVLGATLISDEDKKDAQQYSVVVRVDINVAKLRNAVKGGAPVAMAADGQKSPLTFVFVARQQDSVKSYDPRVYKRADIKESATEKSSRSGYAANTSVSVETGGSRTRKADEIKWKLVPSGNLNTIFTGVFAQSGFQVVEAGYVEPTSGGHLRISALESDFQTGNDLQPATMREAVLGLQTAQVPYLALGTLDIGMQDTDPVTGLTRIYVTVTAKLIDVTTRFPRTVSSVGPVQFSGTGPDASVAQSNALKRAADSAARELAAQLNNAKVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="70" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LBC8</accession>
    <name>Q1LBC8_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">PilY1, Type IV fimbrial biogenesis protein (Signal peptide)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">pilY1</name>
        <name evidence="4" type="ordered locus">Rmet_5689</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="5">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="5">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12548.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011520090.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5689"/>
    <dbReference type="EnsemblBacteria" id="ABF12548">
        <property type="protein sequence ID" value="ABF12548"/>
        <property type="gene ID" value="Rmet_5689"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5689"/>
    <dbReference type="eggNOG" id="COG3419">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004773_1_0_4"/>
    <dbReference type="OMA" id="NQGLVHM"/>
    <dbReference type="OrthoDB" id="165468at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008707">
        <property type="entry name" value="PilC_beta_prop_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002035">
        <property type="entry name" value="VWF_A"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05567">
        <property type="entry name" value="Neisseria_PilC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50234">
        <property type="entry name" value="VWFA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0614">Plasmid</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193034" evidence="2">
        <location>
            <begin position="25"/>
            <end position="1102"/>
        </location>
    </feature>
    <feature type="domain" description="VWFA" evidence="3">
        <location>
            <begin position="41"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="539"/>
            <end position="570"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF12548.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="1102" mass="115484" checksum="8D3908803AFC4D77" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="62" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LBG2</accession>
    <name>Q1LBG2_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_5655</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12514.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011520058.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF12514">
        <property type="protein sequence ID" value="ABF12514"/>
        <property type="gene ID" value="Rmet_5655"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5655"/>
    <dbReference type="HOGENOM" id="CLU_151772_0_0_4"/>
    <dbReference type="OMA" id="PVLTACY"/>
    <dbReference type="OrthoDB" id="2003763at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF12514.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="110" mass="12040" checksum="F2D9D71AFD5AE917" modified="2006-05-30" version="1">MTRLLSIWTLCLGLALASCTYYGYPPGTVPASYDRSFFAAADAMRDQGVAIAVQNQVSGEISGDRGGVPVSAWVRQQPDGSVRVQFNANDSRDPDLVHRISQSYDRRMGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="100" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LBK7</accession>
    <name>Q1LBK7_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Cytochrome bo(3) ubiquinol oxidase subunit 3</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Cytochrome o ubiquinol oxidase subunit 3</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">Oxidase bo(3) subunit 3</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="15">Ubiquinol oxidase polypeptide III</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">Ubiquinol oxidase subunit 3</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="primary">cyoC</name>
        <name evidence="19" type="ordered locus">Rmet_5610</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="20">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="20" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="20">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Cytochrome bo(3) ubiquinol terminal oxidase is the component of the aerobic respiratory chain of E.coli that predominates when cells are grown at high aeration. Has proton pump activity across the membrane in addition to electron transfer, pumping 2 protons/electron.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Heterooctamer of two A chains, two B chains, two C chains and two D chains.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="16">Cell membrane</location>
            <topology evidence="16">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 16">Belongs to the cytochrome c oxidase subunit 3 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12469.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008643180.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5610"/>
    <dbReference type="EnsemblBacteria" id="ABF12469">
        <property type="protein sequence ID" value="ABF12469"/>
        <property type="gene ID" value="Rmet_5610"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5610"/>
    <dbReference type="eggNOG" id="COG1845">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_044071_3_0_4"/>
    <dbReference type="OMA" id="REYAGGP"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009486">
        <property type="term" value="F:cytochrome bo3 ubiquinol oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004129">
        <property type="term" value="F:cytochrome-c oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019646">
        <property type="term" value="P:aerobic electron transport chain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02863">
        <property type="entry name" value="Ubiquinol_oxidase_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024791">
        <property type="entry name" value="Cyt_c/ubiquinol_Oxase_su3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000298">
        <property type="entry name" value="Cyt_c_oxidase-like_su3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035973">
        <property type="entry name" value="Cyt_c_oxidase_su3-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013833">
        <property type="entry name" value="Cyt_c_oxidase_su3_a-hlx"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014206">
        <property type="entry name" value="Cyt_c_ubiqinol_oxidase_su3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033946">
        <property type="entry name" value="Ubiquinol_oxase_su3_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11403">
        <property type="entry name" value="PTHR11403"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00510">
        <property type="entry name" value="COX3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81452">
        <property type="entry name" value="SSF81452"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02842">
        <property type="entry name" value="CyoC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50253">
        <property type="entry name" value="COX3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-0249">Electron transport</keyword>
    <keyword evidence="10 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="19" id="KW-0614">Plasmid</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 16 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 17" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="42"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="82"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="113"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="151"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="195"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="domain" description="COX3" evidence="18">
        <location>
            <begin position="35"/>
            <end position="216"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010581"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011700"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00014687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00025694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00030072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00031884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00032189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00032717"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU003376"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50253"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="ABF12469.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="218" mass="24105" checksum="BA0ECB104DE599F6" modified="2006-05-30" version="1">MVDTTLPLHAHGTADADQAPPGGYQFYLTEEVHPQNGTLLGFWLYLMSDCLIFACLFAAYGVLGREYAGGPSGAELFELPLVALNTSFLLISSITYGFAMLQMQQNRVASTQIWLAITGVFGAAFLTVELYEFAHLIHEGAGPQRSAFLTSFFSLVGTHGLHVTFGIIWLIVLMTQVARHGLITANKRRLMCLSMFWHFLDVVWIGVFTFVYLMGSLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LBQ9</accession>
    <name>Q1LBQ9_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Histone-like DNA-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Rmet_5558</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="5">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="5">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the bacterial histone-like protein family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12417.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519964.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5558"/>
    <dbReference type="EnsemblBacteria" id="ABF12417">
        <property type="protein sequence ID" value="ABF12417"/>
        <property type="gene ID" value="Rmet_5558"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5558"/>
    <dbReference type="eggNOG" id="COG0776">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_105066_0_0_4"/>
    <dbReference type="OMA" id="TGEPMTF"/>
    <dbReference type="OrthoDB" id="1847472at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030527">
        <property type="term" value="F:structural constituent of chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.520.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000119">
        <property type="entry name" value="Hist_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010992">
        <property type="entry name" value="IHF-like_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33175">
        <property type="entry name" value="PTHR33175"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00216">
        <property type="entry name" value="Bac_DNA_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00411">
        <property type="entry name" value="BHL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47729">
        <property type="entry name" value="SSF47729"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0614">Plasmid</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU003939"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF12417.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="140" mass="14801" checksum="BC4E5E02F3974A8B" modified="2006-05-30" version="1">MATKKTAATKPAAKKATAPAKKAAPVAKKAAAAPVAKPLKDTFNKSSLLAHLVAQTELDKKTVQTVLVHLENTIVSAIHKKGAGEFTLPGLFKVAAIQVPATKKRFGKNPFTGQDQWFAAKPASVKVKVRPLKKLKDAAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LC84</accession>
    <name>Q1LC84_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Transcriptional regulator, IclR family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Rmet_5383</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="7">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="7">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12242.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519789.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5383"/>
    <dbReference type="EnsemblBacteria" id="ABF12242">
        <property type="protein sequence ID" value="ABF12242"/>
        <property type="gene ID" value="Rmet_5383"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5383"/>
    <dbReference type="eggNOG" id="COG1414">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_062618_4_0_4"/>
    <dbReference type="OMA" id="CLYREEG"/>
    <dbReference type="OrthoDB" id="370329at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029016">
        <property type="entry name" value="GAF-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014757">
        <property type="entry name" value="Tscrpt_reg_IclR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005471">
        <property type="entry name" value="Tscrpt_reg_IclR_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09339">
        <property type="entry name" value="HTH_IclR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01614">
        <property type="entry name" value="IclR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00346">
        <property type="entry name" value="HTH_ICLR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51077">
        <property type="entry name" value="HTH_ICLR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51078">
        <property type="entry name" value="ICLR_ED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0614">Plasmid</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH iclR-type" evidence="4">
        <location>
            <begin position="24"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="domain" description="IclR-ED" evidence="5">
        <location>
            <begin position="87"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABF12242.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="268" mass="28453" checksum="C02CCD4F2878D3D6" modified="2006-05-30" version="1">MTSRTANRNVRQTDVSSSTSEEGTRALRRGLTLLDAILAAGKDGLRVIDMCRAAGLERPTVYRLLAALIDCGYVVQHGRFRYVRGPRLAAMPQRGESTDLSGKLGPVLTRVSQACGDAAFCVVRDGALSRCIARHVGTHPVQVLVIQVGTRQPLGVGAAGLALLAALPDATVDEVIATNAGVLDQYGGMTPDRMRILIRATRERGYSVIGNHATRGALAVGMAVHDRDGEPVAGISVATTLARMPRERQQLLARVMREAVAALLPCGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="92" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LC88</accession>
    <name>Q1LC88_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Short-chain dehydrogenase putative 3-oxoacyl-(Acyl-carrier-protein) reductase</fullName>
            <ecNumber evidence="1">1.1.1.100</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_5379</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EC" id="1.1.1.100" evidence="1"/>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12238.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519785.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5379"/>
    <dbReference type="EnsemblBacteria" id="ABF12238">
        <property type="protein sequence ID" value="ABF12238"/>
        <property type="gene ID" value="Rmet_5379"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5379"/>
    <dbReference type="eggNOG" id="COG1028">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010194_1_0_4"/>
    <dbReference type="OMA" id="RIWDSKV"/>
    <dbReference type="OrthoDB" id="1356861at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004316">
        <property type="term" value="F:3-oxoacyl-[acyl-carrier-protein] reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00061">
        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF12238.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="269" mass="28066" checksum="3AEAA9FBED0A1619" modified="2006-05-30" version="1">MNALPLSLADRTVFVAGGGSAGPGWSIGRAASVTYARLGARVCVVDRDAASAAETTALIRAEGGAAETFVGDVAEEAEIARLFAEAREHFGPIDVLHHNVGIGKTGGVLDTDVRDFDAAHNVNVRSLLLASQQVLPSMVERGRGAIVTVSSVAGLRYIGYPHLSYSVSKAALIQFTRMIAQQYAGSGIRANCVVPGLIDTPRIAHTVAKQFSADSLDDARAARARQVPMQRMGTAWDVAHACAFLASDAAAYITGTELVVDGGITGKYA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="70" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LCF6</accession>
    <name>Q1LCF6_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Isochorismatase hydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_5311</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12170.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519715.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5311"/>
    <dbReference type="EnsemblBacteria" id="ABF12170">
        <property type="protein sequence ID" value="ABF12170"/>
        <property type="gene ID" value="Rmet_5311"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5311"/>
    <dbReference type="eggNOG" id="COG1335">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_066901_1_0_4"/>
    <dbReference type="OMA" id="DAHDYAM"/>
    <dbReference type="OrthoDB" id="1860541at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.850">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000868">
        <property type="entry name" value="Isochorismatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036380">
        <property type="entry name" value="Isochorismatase-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00857">
        <property type="entry name" value="Isochorismatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52499">
        <property type="entry name" value="SSF52499"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Isochorismatase" evidence="1">
        <location>
            <begin position="15"/>
            <end position="168"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF12170.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="228" mass="25395" checksum="50881B2BD23D8812" modified="2006-05-30" version="1">MSNPKLEVLTPQNSQLIIIDHQPQMAFGVQSMDRQAMKNNVVGLAKAAKIFNIPTTITTVESESFSGFTYPELLDVFPNTKTLERTSMNSWDDQKVRDALKANGRNKVVVAGLWTEVCNTTFALCAMLEGNYEIYMVGDASGGTSKEAHDYAMQRMVQAGAVPVTWQQVLLEWQRDWAHRDTYDAVMKLVKEHSGAYGMGVDYAYTMVHKAAQRTATTHESLAPVPAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-10-07" version="59" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LCI7</accession>
    <name>Q1LCI7_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_5280</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12139.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5280"/>
    <dbReference type="EnsemblBacteria" id="ABF12139">
        <property type="protein sequence ID" value="ABF12139"/>
        <property type="gene ID" value="Rmet_5280"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5280"/>
    <dbReference type="HOGENOM" id="CLU_1304045_0_0_4"/>
    <dbReference type="OrthoDB" id="1880234at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="183"/>
            <end position="211"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF12139.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="211" mass="23275" checksum="301EB4019262AF11" modified="2006-05-30" version="1">MVEHVDIVLAVQRGDATVTALHRHPPDDRQMAGTVLACHAIDHHVYPNGASTMQSTPPAAATLWPMPTFSFQALTSFDHSAMESWHKFMGLNADFTRSMFEEVQFDWASCFVPQDPEDFYVREWSCQIPLLSIPLRYATAMIELTASTQRAWMDAWGHMFGLPVLPTPLSLMPAAVTDMPAAAPKGEVEDVPAASIRETPHPRKGKAASST</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LCJ5</accession>
    <name>Q1LCJ5_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Putative Dimethylmenaquinone methyltransferase-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_5272</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12131.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519677.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5272"/>
    <dbReference type="EnsemblBacteria" id="ABF12131">
        <property type="protein sequence ID" value="ABF12131"/>
        <property type="gene ID" value="Rmet_5272"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5272"/>
    <dbReference type="eggNOG" id="COG0684">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_072626_3_0_4"/>
    <dbReference type="OMA" id="YMPARED"/>
    <dbReference type="OrthoDB" id="1614890at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd16841">
        <property type="entry name" value="RraA_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005493">
        <property type="entry name" value="RraA/RraA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036704">
        <property type="entry name" value="RraA/RraA-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03737">
        <property type="entry name" value="RraA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89562">
        <property type="entry name" value="SSF89562"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF12131.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="239" mass="26093" checksum="5C1A7FBD40A67FFB" modified="2006-05-30" version="1">MSELTPALRAALKTVSTATLCTALFKRGLRNQFIQDVRPIQSNPGTMVGEAFTLRYIPAREDLNPITVFQDPNHPQRQAIEQCPPGAVLVIDSRKDARAASAGSILVTRLMQRGGAGIVTDGGFRDSPEIAQMAMPAYHHRPSAPTNLTLHQALEFNVPIGCGDVAVFPGDVIVGDAEGVVVIPQEMVEDIAAEAVEMTAFEDFVTEEVRKGRSIIGLYPPTTEKAREDFAVWRKENRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="72" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LCM4</accession>
    <name>Q1LCM4_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_5243</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12102.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519648.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5243"/>
    <dbReference type="EnsemblBacteria" id="ABF12102">
        <property type="protein sequence ID" value="ABF12102"/>
        <property type="gene ID" value="Rmet_5243"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5243"/>
    <dbReference type="eggNOG" id="COG2227">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1208169_0_0_4"/>
    <dbReference type="OMA" id="HYQYLEY"/>
    <dbReference type="OrthoDB" id="1718441at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF12102.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="231" mass="26184" checksum="87A8037F531255DD" modified="2006-05-30" version="1">MKRIAHYQYLEYVRVMSLMRQYQAASAKPLRVLDFGCGLGKFLNVFASLGMEVTGVDANPAYVAGAREKGFQAYEPEAFFATTQDPFDVVFLSHLIEHVTPDALVALVPRLCAQLARNGRMIMLSPTPGERFYHDFSHIRPYLPQSIRHAFGATGMPISYGEAGLLEMVDIHFFKDPYRTRLWRSFYVNRGIKGAFTRQYNRLLDVLWSASGGRVGKTASWLGVYELRARS</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LD35</accession>
    <name>Q1LD35_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">OmpA-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Rmet_5082</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="6">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="6">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11941.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519488.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5082"/>
    <dbReference type="EnsemblBacteria" id="ABF11941">
        <property type="protein sequence ID" value="ABF11941"/>
        <property type="gene ID" value="Rmet_5082"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5082"/>
    <dbReference type="eggNOG" id="COG1360">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1265694_0_0_4"/>
    <dbReference type="OMA" id="NRQFADN"/>
    <dbReference type="OrthoDB" id="1619550at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd07185">
        <property type="entry name" value="OmpA_C-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006665">
        <property type="entry name" value="OmpA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036737">
        <property type="entry name" value="OmpA-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00691">
        <property type="entry name" value="OmpA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103088">
        <property type="entry name" value="SSF103088"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51123">
        <property type="entry name" value="OMPA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 3" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0614">Plasmid</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="15"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="domain" description="OmpA-like" evidence="4">
        <location>
            <begin position="78"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="184"/>
            <end position="217"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABF11941.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="217" mass="23105" checksum="977ECC818C94DD74" modified="2006-05-30" version="1">MTDDFDAGAEPTVPAWAVFGDLMSVMLGAFVLVLLGVIGVQMELSARLENEVKQRQEELKRRQTLEEALAGPLAAGRVTLKDGRIGISGNVLFALNSDQLQPEGRALLKSLAAPLSAYLGARDEILMVSGFTDDQQVREGNRRFADNLELSAQRALTVTRALIEAGLPPASIFSAAFGSQQPVASNATEESRAKNRRVEIAPVPRVSAGAGATKPHA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LD77</accession>
    <name>Q1LD77_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative thioesterase/thiol ester dehydrase-isomerase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_5037</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the thioesterase PaaI family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11899.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519450.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5037"/>
    <dbReference type="EnsemblBacteria" id="ABF11899">
        <property type="protein sequence ID" value="ABF11899"/>
        <property type="gene ID" value="Rmet_5037"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5037"/>
    <dbReference type="eggNOG" id="COG2050">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_089876_5_1_4"/>
    <dbReference type="OMA" id="HAGMVST"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047617">
        <property type="term" value="F:acyl-CoA hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016853">
        <property type="term" value="F:isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039298">
        <property type="entry name" value="ACOT13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029069">
        <property type="entry name" value="HotDog_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003736">
        <property type="entry name" value="PAAI_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006683">
        <property type="entry name" value="Thioestr_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21660">
        <property type="entry name" value="PTHR21660"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03061">
        <property type="entry name" value="4HBT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54637">
        <property type="entry name" value="SSF54637"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00369">
        <property type="entry name" value="unchar_dom_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0413">Isomerase</keyword>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="4HBT" evidence="2">
        <location>
            <begin position="51"/>
            <end position="124"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF03061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF11899.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="146" mass="15421" checksum="0BC269DF9A62C722" modified="2006-05-30" version="1">MTPANANFVEAVQASFQSQNVMHLIRATLPVVEAGRTEIHVPHWDGIEQQHGYVHGGVVGTIADSAGGYAAMSLVPAGASVLTVEYKLNLLAPANGDKLIARGSVVRAGRTLIVTQAEVFAVEGPKETLCALMQQTIMVMQGKAEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="65" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LDL5</accession>
    <name>Q1LDL5_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_4899</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11761.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519321.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4899"/>
    <dbReference type="EnsemblBacteria" id="ABF11761">
        <property type="protein sequence ID" value="ABF11761"/>
        <property type="gene ID" value="Rmet_4899"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4899"/>
    <dbReference type="HOGENOM" id="CLU_1438903_0_0_4"/>
    <dbReference type="OMA" id="YWARGAK"/>
    <dbReference type="OrthoDB" id="1648114at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193381" evidence="2">
        <location>
            <begin position="20"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="36"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="40"/>
            <end position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF11761.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="178" mass="19141" checksum="A2031D2359A0A13B" modified="2006-05-30" version="1">MKTLALFCAALLTSAVAHAETLNAAAPLGVELGKTTCTRMPSGPDRTKTGTSPWAGGDTIEIRNLERFKLPGLTKATVNCDQHDTVALVTLNFDRSAMDDVVRQLDARYESKRKTDANAENGYAEWAAANGGIEMLYARDGKALVVAYWAKGAKAKYFSYSATRDKQPPAAVTPQGQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="102" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LE45</accession>
    <name>Q1LE45_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Transcriptional regulator, AraC family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Rmet_4719</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="6">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="6">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11581.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519153.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4719"/>
    <dbReference type="EnsemblBacteria" id="ABF11581">
        <property type="protein sequence ID" value="ABF11581"/>
        <property type="gene ID" value="Rmet_4719"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4719"/>
    <dbReference type="eggNOG" id="COG4977">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_88_15_4"/>
    <dbReference type="OMA" id="YPDQTTF"/>
    <dbReference type="OrthoDB" id="1268974at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018060">
        <property type="entry name" value="HTH_AraC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018062">
        <property type="entry name" value="HTH_AraC-typ_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011051">
        <property type="entry name" value="RmlC_Cupin_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12833">
        <property type="entry name" value="HTH_18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00342">
        <property type="entry name" value="HTH_ARAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51182">
        <property type="entry name" value="SSF51182"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00041">
        <property type="entry name" value="HTH_ARAC_FAMILY_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01124">
        <property type="entry name" value="HTH_ARAC_FAMILY_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0614">Plasmid</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH araC/xylS-type" evidence="4">
        <location>
            <begin position="154"/>
            <end position="252"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS01124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABF11581.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="255" mass="27738" checksum="C2848BBCF8E4F9BE" modified="2006-05-30" version="1">MPKLVEHASPLVEPRRYSPRPFGHAHDYHQLLFGVDGAIELQIDGHAYRVDDQHGLVVPAGAHHVCAGLTENLQLVADFPASSVALPARLMENPRAFAMDASFAARVRALATHRPSGDLPLQHAWHCATALAGNLASALGLDAARNDATHFPAMAIDAWLRANLASPLRVDQLAARMGWGARRFHTLFCEAFGDTPHGYQTRLRLDQAVQWLMAGTMPLADIAYGVGYPDQTTFTRAFARRFGKPPGAWRAAALA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LE84</accession>
    <name>Q1LE84_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Short chain fatty acid transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">atoE</name>
        <name evidence="2" type="ordered locus">Rmet_4680</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11542.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519114.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4680"/>
    <dbReference type="EnsemblBacteria" id="ABF11542">
        <property type="protein sequence ID" value="ABF11542"/>
        <property type="gene ID" value="Rmet_4680"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4680"/>
    <dbReference type="eggNOG" id="COG2031">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037744_0_0_4"/>
    <dbReference type="OMA" id="CAYIAFM"/>
    <dbReference type="OrthoDB" id="350103at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006160">
        <property type="entry name" value="SCFA_transpt_AtoE"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR41983">
        <property type="entry name" value="PTHR41983"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02667">
        <property type="entry name" value="SCFA_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="53"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="96"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="135"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="181"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="247"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="277"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="307"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="345"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="419"/>
            <end position="443"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF11542.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="445" mass="47779" checksum="E6F069EED4D1F458" modified="2006-05-30" version="1">MSKVAGFFTELMRRYLPDPFVFAIGLTLLTMVMAMLVEGQAAQAVITNWGKGFWNLLSFTTQMAVILAMGYVLATAPLVDRFLNAIVARVQTPRGAIIVATLVGGIGSYLNWGFGLVIGGIIARKLALKVKGVHYPLIIASAYSGFTMYGLGLSASIPVLISTPGHPMEKLMGVIGLKETIFSTPMLLTSLAVIVTLPLLNAMLHPRDAKEVVEIREENSSEDRAAGHGHSMGIENTLATRMNNSRLLSLIIGLCGLAYAGLHFSQGGSLDLNLINFLILFIGVLLLGTPANYVAKLNEGIKTISGIILQYPFYAGIMAVMAGSGLVDSISRVFVDIATPQTLPFWGLVSSFVINFFAPSGGGHWVIQGPFMIDAAKAINSSVAQTSMSVMLGNAWNDLVQPFWILPALALSKLKLKDVMGYTVIMMFWVGAIYIVAMLAWGAQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LEC7</accession>
    <name>Q1LEC7_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_4637</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11499.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519073.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4637"/>
    <dbReference type="EnsemblBacteria" id="ABF11499">
        <property type="protein sequence ID" value="ABF11499"/>
        <property type="gene ID" value="Rmet_4637"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4637"/>
    <dbReference type="eggNOG" id="COG1977">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_114601_0_1_4"/>
    <dbReference type="OMA" id="MPRIVFA"/>
    <dbReference type="OrthoDB" id="1995573at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012675">
        <property type="entry name" value="Beta-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016155">
        <property type="entry name" value="Mopterin_synth/thiamin_S_b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003749">
        <property type="entry name" value="ThiS/MoaD-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02597">
        <property type="entry name" value="ThiS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54285">
        <property type="entry name" value="SSF54285"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF11499.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="89" mass="9883" checksum="EA58DA69EF935713" modified="2006-05-30" version="1">MAHIFFAASIQRHIPTPEREVDARTLGEALEAVFADRPQLRGYILDDQGALRRHLAVFVDGQRVLDRQHLSDALGEKSQVHVIQALSGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="99" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LEF8</accession>
    <name>Q1LEF8_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">O-acetylhomoserine/O-acetylserine sulfhydrylase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">metY</name>
        <name evidence="5" type="ordered locus">Rmet_4606</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="6">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="6">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 4">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 4">Belongs to the trans-sulfuration enzymes family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11468.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519043.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4606"/>
    <dbReference type="EnsemblBacteria" id="ABF11468">
        <property type="protein sequence ID" value="ABF11468"/>
        <property type="gene ID" value="Rmet_4606"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4606"/>
    <dbReference type="eggNOG" id="COG2873">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018986_4_0_4"/>
    <dbReference type="OMA" id="TYTLFAH"/>
    <dbReference type="OrthoDB" id="637281at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016765">
        <property type="term" value="F:transferase activity, transferring alkyl or aryl (other than methyl) groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019346">
        <property type="term" value="P:transsulfuration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00614">
        <property type="entry name" value="CGS_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000277">
        <property type="entry name" value="Cys/Met-Metab_PyrdxlP-dep_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006235">
        <property type="entry name" value="OAc-hSer/O-AcSer_sulfhydrylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01053">
        <property type="entry name" value="Cys_Met_Meta_PP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001434">
        <property type="entry name" value="CGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="SSF53383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01326">
        <property type="entry name" value="OAH_OAS_sulfhy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00868">
        <property type="entry name" value="CYS_MET_METAB_PP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3 4" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="3">
        <location>
            <position position="208"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR001434-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU362118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABF11468.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="431" mass="45996" checksum="DCD0B3AD2F51C228" modified="2006-05-30" version="1">MSENWKFETRSVHAGYSPDPTTKAVAVPIYQTAAYAFDNAQHGADLFDLKVPGNIYTRIMNPTTDVLEKRIAALEGGVGALAVASGQAAVTAAIQTITEAGDNIVASSALYGGTYNLLAHTMPQFGVTTRFADHRCPESFEALIDDRTKAVFVESIGNPAGNVTDIAKIAAIAKRHGVPVIVDNTVATPYLLRPFEHGADIVVHSLTKYLGGHGTSIGGAIVDSGKFPWAKYPSRFPKLNTPDASYHGVTYTEAFGPAAYIGRARVVPLRNMGAALSPFNAFLILQGIETLGLRMDRINDNTLAVARHLSQHAKVGWVNYAGLEDNQDYALAKKYMNGKASGLLTFGVKAADGDGRAAGARFLDALQLFTRLVNIGDVRSLATHPASTTHRQLSPEELAQTGVGLDTVRLCVGIEHIDDLLADIDQALQAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="62" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LEM5</accession>
    <name>Q1LEM5_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Protein involved in meta-pathway of phenol degradation</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_4536</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11401.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518992.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4536"/>
    <dbReference type="EnsemblBacteria" id="ABF11401">
        <property type="protein sequence ID" value="ABF11401"/>
        <property type="gene ID" value="Rmet_4536"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4536"/>
    <dbReference type="eggNOG" id="COG4313">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_066206_0_0_4"/>
    <dbReference type="OMA" id="IIAGYHF"/>
    <dbReference type="OrthoDB" id="1322015at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025737">
        <property type="entry name" value="Phenol_MetA_deg_put"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13557">
        <property type="entry name" value="Phenol_MetA_deg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193082" evidence="1">
        <location>
            <begin position="26"/>
            <end position="318"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF11401.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="318" mass="33969" checksum="65B4F45957838E6B" modified="2006-05-30" version="1">MKRAQSAVLTAVAAGGLLAPLACHATEGALGRPVTGTSVSPNVAVIPSEPVWIANLQQLYMDGSITGGREVPIAGKTSIGLDAKVAFTLATLMKVWDTGGGAWNFASSFTLPYVWMSVHTTAGIGNFSRGTSDRASNLYDMYFSPIIAGYHFSKTSHMALSFNVWAPTGQYDKNALANPSLNNWTFVPQIAFTHLMPEYGLEFNAVGAFQFYTRNTATDYQNAPLFTLDVMGVKKFGKWGAGIVMGTVQQLGNDSGPIADRLNGFRGRDFAMGPIVTYDTKLGGKAPLSLSLRWVPTVASKNRLDSTATVMGSATLVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LEX2</accession>
    <name>Q1LEX2_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">N-acyl-D-amino-acid deacylase</fullName>
            <ecNumber evidence="2">3.5.1.81</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_4439</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EC" id="3.5.1.81" evidence="2"/>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11304.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518910.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4439"/>
    <dbReference type="EnsemblBacteria" id="ABF11304">
        <property type="protein sequence ID" value="ABF11304"/>
        <property type="gene ID" value="Rmet_4439"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4439"/>
    <dbReference type="eggNOG" id="COG3653">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016107_2_0_4"/>
    <dbReference type="OMA" id="GNCGFTL"/>
    <dbReference type="OrthoDB" id="1319925at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047420">
        <property type="term" value="F:N-acyl-D-amino-acid deacylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013108">
        <property type="entry name" value="Amidohydro_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023100">
        <property type="entry name" value="D-aminoacylase_insert_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011059">
        <property type="entry name" value="Metal-dep_hydrolase_composite"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07969">
        <property type="entry name" value="Amidohydro_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51338">
        <property type="entry name" value="SSF51338"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="SSF51556"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Amidohydro_3" evidence="1">
        <location>
            <begin position="46"/>
            <end position="460"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF07969"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF11304.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="477" mass="50341" checksum="C1ACA81356F1A55A" modified="2006-05-30" version="1">MPSCDLLFTNATLIDGTGATRRTGALAVTDGRIVALGDCSGWQAARTVDATGHVLAPGFIDVHTHDDSALLSTPAMAAKTSQGVTSVIAGNCGISIAPLTPTHGLPQPLMLLGEVSDYRFPSVADYRRALEASPPSVNVGLLVGHSTLRAGAMTDTTRAATAEEIEAMAAMLDQALSDGGLGMSTGLSYPASRAAPTAEVVALARVTARHPGAIYTTHMRSERDQVIEAVGETLEIGREAALPVVISHHKCIGRKNFGKTTVTLSLIDKALATQDVSLDVYPYTASSTVLLPEFVGEAERIIVTSSQPYPEQARRDLSAIAADWNVSLEEAAARLGPATAIYFQMDEGDLRRVLSHPRTMIGSDGIPGPYPHPRLWGSFPRVLGHYARELGLLTLEDAVRRMTSLPASVFGLPGRGVLEVGYAADLVLFDPEQVIDRADYTQPCEPSAGIEAVYVNGVQVWGPDGATGAAAGQFLRH</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-10-07" version="60" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LF35</accession>
    <name>Q1LF35_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Ketoacyl_synth_N domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_4376</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11241.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4376"/>
    <dbReference type="EnsemblBacteria" id="ABF11241">
        <property type="protein sequence ID" value="ABF11241"/>
        <property type="gene ID" value="Rmet_4376"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4376"/>
    <dbReference type="eggNOG" id="ENOG5032YZ0">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_086378_1_0_4"/>
    <dbReference type="OMA" id="MMRRRMS"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014030">
        <property type="entry name" value="Ketoacyl_synth_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13723">
        <property type="entry name" value="Ketoacyl-synt_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ketoacyl_synth_N" evidence="1">
        <location>
            <begin position="48"/>
            <end position="282"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF11241.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="286" mass="30833" checksum="A56E8CF16303237A" modified="2006-05-30" version="1">MRRRPDIQPIRIWRRTGSQSSHAQGMTLEFEIRAWAACAPALQTHADWLAWSRAPWLPEGADLPALAAMAPMLRRRVNATGRAALHPAYEIAANDAGVMPAVFASRHGDSRRAFSMLSELAAGEPLSPTAFGLSVHNAIGAMFSIDRSAASNIQALSGGKDTVEAAIVEACSLLADGADEVLLVCYDTPLPEPYLTYADEPQCLYGWAWRVGLPRAGAPVWTLELADPAIEVTDKSGDGPVDGQGKPLLPHGLEVLHFVLSNDAGAGKSQLLHRTDHAIWKWGRHV</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="97" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LFR7</accession>
    <name>Q1LFR7_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Putative transcriptional regulator, IclR family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Rmet_4142</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="6">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="6">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11009.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518634.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4142"/>
    <dbReference type="EnsemblBacteria" id="ABF11009">
        <property type="protein sequence ID" value="ABF11009"/>
        <property type="gene ID" value="Rmet_4142"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4142"/>
    <dbReference type="eggNOG" id="COG1414">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_062618_4_1_4"/>
    <dbReference type="OMA" id="CLYRVDS"/>
    <dbReference type="OrthoDB" id="370329at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029016">
        <property type="entry name" value="GAF-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014757">
        <property type="entry name" value="Tscrpt_reg_IclR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005471">
        <property type="entry name" value="Tscrpt_reg_IclR_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09339">
        <property type="entry name" value="HTH_IclR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00346">
        <property type="entry name" value="HTH_ICLR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51077">
        <property type="entry name" value="HTH_ICLR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51078">
        <property type="entry name" value="ICLR_ED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0614">Plasmid</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH iclR-type" evidence="3">
        <location>
            <begin position="20"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="domain" description="IclR-ED" evidence="4">
        <location>
            <begin position="82"/>
            <end position="236"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABF11009.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="258" mass="27595" checksum="42AB1BB1537BB36A" modified="2006-05-30" version="1">MPRKAAQLSEADKHAAEGGAVAVDRALFVLSAFREGDGTLGLAELAQRSGLYKSTLLRLLASLEHARLIQRHTDGRYGLGPEIARLNAVYAASFSLEQVVLPALRELVRTTRESAAFHIQQGEHRLCLYRVDSPQPVRDHIRAGDLLPLNRGAGGRVLRAFAGARGEIYQRIRDEGVIALVGDRSPDIAGVSSPVFGPGGELKGALTLTCPTPRFSESFREQVLDAARRLTQALGGIFPEVVEAAGPSSSAATAPQDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-10-07" version="58" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LG10</accession>
    <name>Q1LG10_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_4048</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10916.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4048"/>
    <dbReference type="EnsemblBacteria" id="ABF10916">
        <property type="protein sequence ID" value="ABF10916"/>
        <property type="gene ID" value="Rmet_4048"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4048"/>
    <dbReference type="eggNOG" id="COG2203">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_120803_0_0_4"/>
    <dbReference type="OMA" id="LLTINAW"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029016">
        <property type="entry name" value="GAF-like_dom_sf"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF10916.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="172" mass="18214" checksum="A41994448D94F912" modified="2006-05-30" version="1">MVDAANMTLPMHWQAQLNALLDRLRDAATPQGAMDAVGQATLAMVGPGLLTINAWHVRTGEIERLWSSDSAAYPVGGKKIKGDTAWTRQLLVRGEVFVGEGDEALAAVFDDIAVIRGLGLNGVVNVPLSRRGAVIGTFNYLADVQAWTADEITALRLLGQLVAPAVVEVIAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LGQ5</accession>
    <name>Q1LGQ5_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Extra-cytoplasmic solute receptor protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">bug</name>
        <name evidence="4" type="ordered locus">Rmet_3801</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="5">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="5">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Periplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the UPF0065 (bug) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10671.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518320.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3801"/>
    <dbReference type="EnsemblBacteria" id="ABF10671">
        <property type="protein sequence ID" value="ABF10671"/>
        <property type="gene ID" value="Rmet_3801"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3801"/>
    <dbReference type="eggNOG" id="COG3181">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045683_0_0_4"/>
    <dbReference type="OMA" id="GYEAYSW"/>
    <dbReference type="OrthoDB" id="447734at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042597">
        <property type="term" value="C:periplasmic space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005064">
        <property type="entry name" value="BUG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042100">
        <property type="entry name" value="Bug_dom1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42928">
        <property type="entry name" value="PTHR42928"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03401">
        <property type="entry name" value="TctC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017082">
        <property type="entry name" value="YflP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-0675">Receptor</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193528" evidence="3">
        <location>
            <begin position="31"/>
            <end position="330"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF10671.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="330" mass="35087" checksum="C7570D0313041596" modified="2006-05-30" version="1">MQSPRSFAMRRRLLAAGLLGAVAHPLSALAADTWPSKPVRMIVPFPPGGPVDTTARIFGQKLGEMWKVPVVIDNRPGAGGVIGATVAAKEPADGYSLFVGAIHHSVNPSLMGKLPYDIEKDFAPVSFATMYPIFVVVHPSVPANNIKEFIAYAKKSDKPLAFGSSGNGGGTHLAGELFNMEAGTKLQHIPYKGSAPAMSDLLGGQVQVMFSDAPTALPHIKTGRIKVLGVASRQRSAILPDVPTVAESGLPGYEAYSWSALFAPAHTPQPILNKLNADFNVAMNDPAVRQRMVQAGADADPGTQEQMRQRLHSEIEKWKKVIQTAGITAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="97" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LGX5</accession>
    <name>Q1LGX5_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Isocitrate dehydrogenase [NADP]</fullName>
            <ecNumber evidence="1">1.1.1.42</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Oxalosuccinate decarboxylase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5" type="primary">icdA</name>
        <name evidence="5" type="ordered locus">Rmet_3729</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="6">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="6">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>D-threo-isocitrate + NADP(+) = 2-oxoglutarate + CO2 + NADPH</text>
            <dbReference type="Rhea" id="RHEA:19629"/>
            <dbReference type="ChEBI" id="CHEBI:15562"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.1.1.42"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="4">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <cofactor evidence="4">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="4">Binds 1 Mg(2+) or Mn(2+) ion per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the monomeric-type IDH family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.42" evidence="1"/>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10601.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518249.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3729"/>
    <dbReference type="EnsemblBacteria" id="ABF10601">
        <property type="protein sequence ID" value="ABF10601"/>
        <property type="gene ID" value="Rmet_3729"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3729"/>
    <dbReference type="eggNOG" id="COG2838">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025308_1_0_4"/>
    <dbReference type="OMA" id="FRMCQTK"/>
    <dbReference type="OrthoDB" id="222705at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004450">
        <property type="term" value="F:isocitrate dehydrogenase (NADP+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006097">
        <property type="term" value="P:glyoxylate cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004436">
        <property type="entry name" value="Isocitrate_DH_NADP_mono"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36999">
        <property type="entry name" value="PTHR36999"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03971">
        <property type="entry name" value="IDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF009407">
        <property type="entry name" value="IDH_monmr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00178">
        <property type="entry name" value="monomer_idh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0329">Glyoxylate bypass</keyword>
    <keyword evidence="4" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1" id="KW-0521">NADP</keyword>
    <keyword evidence="1 5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-0614">Plasmid</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0816">Tricarboxylic acid cycle</keyword>
    <feature type="region of interest" description="Substrate binding" evidence="3">
        <location>
            <begin position="134"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="147"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="552"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Critical for catalysis" evidence="2">
        <location>
            <position position="257"/>
        </location>
    </feature>
    <feature type="site" description="Critical for catalysis" evidence="2">
        <location>
            <position position="422"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRNR" id="PIRNR009407"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR009407-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR009407-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR009407-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABF10601.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="747" mass="82169" checksum="2EB30EADADE1E386" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LH23</accession>
    <name>Q1LH23_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Purine-binding chemotaxis protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">cheW</name>
        <name evidence="3" type="ordered locus">Rmet_3681</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10553.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3681"/>
    <dbReference type="EnsemblBacteria" id="ABF10553">
        <property type="protein sequence ID" value="ABF10553"/>
        <property type="gene ID" value="Rmet_3681"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3681"/>
    <dbReference type="eggNOG" id="COG0835">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_048995_1_0_4"/>
    <dbReference type="OMA" id="CVTRIAN"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006935">
        <property type="term" value="P:chemotaxis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039315">
        <property type="entry name" value="CheW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036061">
        <property type="entry name" value="CheW-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002545">
        <property type="entry name" value="CheW-lke_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22617">
        <property type="entry name" value="PTHR22617"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01584">
        <property type="entry name" value="CheW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00260">
        <property type="entry name" value="CheW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50341">
        <property type="entry name" value="SSF50341"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50851">
        <property type="entry name" value="CHEW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CheW-like" evidence="2">
        <location>
            <begin position="33"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="8"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50851"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF10553.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="183" mass="19689" checksum="38E43127E1B2BE9C" modified="2006-05-30" version="1">MRGNGKGTPARQRSTMPAGATMNISINRTDGSGEEFLAFRLGREEYGIDILKVQEIRGYETVTQIANAPAYLKGVINLRGTIVPIIDLRIKFNQAQISYDQYTVVIIVDIHERTTGIVVDGVSDVLTLAPAQIKQAPTLSGGVETDYIRGIGSLEGRMLILADIEKLMNVDDLAAIEAAAGAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LHI1</accession>
    <name>Q1LHI1_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">DNA-binding transcriptional activator of copper-responsive regulon genes</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">cupR</name>
        <name evidence="5" type="ordered locus">Rmet_3523</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10395.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008648660.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="SMR" id="Q1LHI1"/>
    <dbReference type="STRING" id="266264.Rmet_3523"/>
    <dbReference type="EnsemblBacteria" id="ABF10395">
        <property type="protein sequence ID" value="ABF10395"/>
        <property type="gene ID" value="Rmet_3523"/>
    </dbReference>
    <dbReference type="GeneID" id="60822628"/>
    <dbReference type="KEGG" id="rme:Rmet_3523"/>
    <dbReference type="eggNOG" id="COG0789">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_060077_2_0_4"/>
    <dbReference type="OMA" id="FNDPERH"/>
    <dbReference type="OrthoDB" id="1661714at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045893">
        <property type="term" value="P:positive regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01108">
        <property type="entry name" value="HTH_CueR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011789">
        <property type="entry name" value="CueR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009061">
        <property type="entry name" value="DNA-bd_dom_put_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000551">
        <property type="entry name" value="MerR-type_HTH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015358">
        <property type="entry name" value="Tscrpt_reg_MerR_DNA-bd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00376">
        <property type="entry name" value="MerR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09278">
        <property type="entry name" value="MerR-DNA-bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00040">
        <property type="entry name" value="HTHMERR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00422">
        <property type="entry name" value="HTH_MERR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46955">
        <property type="entry name" value="SSF46955"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02044">
        <property type="entry name" value="CueR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00552">
        <property type="entry name" value="HTH_MERR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50937">
        <property type="entry name" value="HTH_MERR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH merR-type" evidence="4">
        <location>
            <begin position="1"/>
            <end position="69"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABF10395.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="139" mass="15452" checksum="6A89ADD41B8EFA8A" modified="2006-05-30" version="1">MNIGEAAEASGVSAKMIRHYESIGLVDAPPRSEGGYRRYDERAVHTLRFVRRARNLGFSLDEIRGLLSLWHDRARASADVKALTLKHVADLEVRIAELAAMRDTLRSLAEACTGDDRPDCPILSDMARADSEDRPACHH</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LHU7</accession>
    <name>Q1LHU7_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Glycosyl transferase, family 2-protein</fullName>
            <ecNumber evidence="6">2.4.1.80</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Rmet_3407</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Lipid metabolism.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.80" evidence="6"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10279.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3407"/>
    <dbReference type="CAZy" id="GT21">
        <property type="family name" value="Glycosyltransferase Family 21"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF10279">
        <property type="protein sequence ID" value="ABF10279"/>
        <property type="gene ID" value="Rmet_3407"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3407"/>
    <dbReference type="eggNOG" id="COG1215">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030898_2_0_4"/>
    <dbReference type="OMA" id="NPKLNNC"/>
    <dbReference type="UniPathway" id="UPA00109">
        <property type="reaction ID" value="UER00187"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008120">
        <property type="term" value="F:ceramide glucosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102769">
        <property type="term" value="F:dihydroceramide glucosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025993">
        <property type="entry name" value="Ceramide_glucosylTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017835">
        <property type="entry name" value="Hopen-assoc_HpnI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12726">
        <property type="entry name" value="PTHR12726"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13506">
        <property type="entry name" value="Glyco_transf_21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="SSF53448"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03472">
        <property type="entry name" value="HpnI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 6" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="266"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="295"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="344"/>
            <end position="380"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABF10279.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="380" mass="41368" checksum="329CFAB951C53B44" modified="2006-05-30" version="1">MVEAATVYAVVAACVSRKRPAQTATPSHLAPVSVLKPLCGAEPHLYENLAGLCRQDYPDYQLVFGVCAYDDPAITVVNLLRTAFPERDIVLIVDARTHGRNPKVSNLINLYRAARHAHLVIADSDIAVPPDYLRRLAGPLSDPHVGVVTCLYRGRPVDGIWSRLGAAFINEWFAPSVRVAHAGGSRRFAFGATIALRRDTLNAIGGFRALANRLADDYWLGELTRQSGWKTVLSDVVVETDVTETSLRALWRHELRWLRTIRSLNASGFFFTFLTCTWPMLVLGCLLAPHLPVLAIALVGALARSVIAGSIGAALRAPLRDALLLVEWAAALGGGRVHWRGHVMTTTDAPPTHSTAQPGNRPALPKPLYSTTPHAQDSPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LHW7</accession>
    <name>Q1LHW7_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">General secretion pathway protein J</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">gspJ</name>
        <name evidence="2" type="ordered locus">Rmet_3387</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10259.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517822.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3387"/>
    <dbReference type="EnsemblBacteria" id="ABF10259">
        <property type="protein sequence ID" value="ABF10259"/>
        <property type="gene ID" value="Rmet_3387"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3387"/>
    <dbReference type="eggNOG" id="COG4795">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_102874_0_0_4"/>
    <dbReference type="OMA" id="FQSDCEH"/>
    <dbReference type="OrthoDB" id="1213602at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012902">
        <property type="entry name" value="N_methyl_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045584">
        <property type="entry name" value="Pilin-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07963">
        <property type="entry name" value="N_methyl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54523">
        <property type="entry name" value="SSF54523"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02532">
        <property type="entry name" value="IV_pilin_GFxxxE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00409">
        <property type="entry name" value="PROKAR_NTER_METHYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF10259.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="230" mass="25043" checksum="F5A1EEB046A9E0AB" modified="2006-05-30" version="1">MSSDRGRGFTLLEMLVAITLLAVMAVIGWRALDSMTRTRERLVDHDKRLDTLKVLYGQFQSDCEHLTLPALLQASPVELAQNQILMVRDRRDAGSPPAWQVVSYRLDGNTVVRAASRPAMSRNEVQNAIQSMRQTAATTGVQLRPLLPDAEQLSARVWVEPSGWLTDTGRIRAALQQGSAAAAVGASDAQPGLDLVAKTVAIRAVELTVLARMGDGDVPRQFQKICTTGL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2007-09-11" modified="2022-08-03" version="92" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LI20</accession>
    <name>RPOB_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DNA-directed RNA polymerase subunit beta</fullName>
            <shortName evidence="1">RNAP subunit beta</shortName>
            <ecNumber evidence="1">2.7.7.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">RNA polymerase subunit beta</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Transcriptase subunit beta</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpoB</name>
        <name type="ordered locus">Rmet_3334</name>
    </gene>
    <organism>
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate + RNA(n+1)</text>
            <dbReference type="Rhea" id="RHEA:21248"/>
            <dbReference type="Rhea" id="RHEA-COMP:14527"/>
            <dbReference type="Rhea" id="RHEA-COMP:17342"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61557"/>
            <dbReference type="ChEBI" id="CHEBI:140395"/>
            <dbReference type="EC" id="2.7.7.6"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta' and 1 omega subunit. When a sigma factor is associated with the core the holoenzyme is formed, which can initiate transcription.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the RNA polymerase beta chain family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.6" evidence="1"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10206.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008649192.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q1LI20"/>
    <dbReference type="SMR" id="Q1LI20"/>
    <dbReference type="STRING" id="266264.Rmet_3334"/>
    <dbReference type="PRIDE" id="Q1LI20"/>
    <dbReference type="EnsemblBacteria" id="ABF10206">
        <property type="protein sequence ID" value="ABF10206"/>
        <property type="gene ID" value="Rmet_3334"/>
    </dbReference>
    <dbReference type="GeneID" id="60826583"/>
    <dbReference type="KEGG" id="rme:Rmet_3334"/>
    <dbReference type="eggNOG" id="COG0085">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000524_4_0_4"/>
    <dbReference type="OMA" id="FMTWEGY"/>
    <dbReference type="OrthoDB" id="9601at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000428">
        <property type="term" value="C:DNA-directed RNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed 5'-3' RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032549">
        <property type="term" value="F:ribonucleoside binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00653">
        <property type="entry name" value="RNA_pol_B_RPB2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.150.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.270.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1110.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01321">
        <property type="entry name" value="RNApol_bact_RpoB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042107">
        <property type="entry name" value="DNA-dir_RNA_pol_bsu_ext_1_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019462">
        <property type="entry name" value="DNA-dir_RNA_pol_bsu_external_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015712">
        <property type="entry name" value="DNA-dir_RNA_pol_su2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007120">
        <property type="entry name" value="DNA-dir_RNAP_su2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037033">
        <property type="entry name" value="DNA-dir_RNAP_su2_hyb_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010243">
        <property type="entry name" value="RNA_pol_bsu_bac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007121">
        <property type="entry name" value="RNA_pol_bsu_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007644">
        <property type="entry name" value="RNA_pol_bsu_protrusion"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007642">
        <property type="entry name" value="RNA_pol_Rpb2_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037034">
        <property type="entry name" value="RNA_pol_Rpb2_2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007645">
        <property type="entry name" value="RNA_pol_Rpb2_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007641">
        <property type="entry name" value="RNA_pol_Rpb2_7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014724">
        <property type="entry name" value="RNA_pol_RPB2_OB-fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20856">
        <property type="entry name" value="PTHR20856"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04563">
        <property type="entry name" value="RNA_pol_Rpb2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04561">
        <property type="entry name" value="RNA_pol_Rpb2_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04565">
        <property type="entry name" value="RNA_pol_Rpb2_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10385">
        <property type="entry name" value="RNA_pol_Rpb2_45"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00562">
        <property type="entry name" value="RNA_pol_Rpb2_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04560">
        <property type="entry name" value="RNA_pol_Rpb2_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02013">
        <property type="entry name" value="rpoB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01166">
        <property type="entry name" value="RNA_POL_BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0804">Transcription</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000300381" description="DNA-directed RNA polymerase subunit beta">
        <location>
            <begin position="1"/>
            <end position="1368"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01321"/>
        </source>
    </evidence>
    <sequence length="1368" mass="152496" checksum="7726E8CFF266CA4D" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2007-07-10" modified="2022-05-25" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LI61</accession>
    <name>RS11_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">30S ribosomal protein S11</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpsK</name>
        <name type="ordered locus">Rmet_3293</name>
    </gene>
    <organism>
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Located on the platform of the 30S subunit, it bridges several disparate RNA helices of the 16S rRNA. Forms part of the Shine-Dalgarno cleft in the 70S ribosome.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the 30S ribosomal subunit. Interacts with proteins S7 and S18. Binds to IF-3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uS11 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10165.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008642963.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q1LI61"/>
    <dbReference type="SMR" id="Q1LI61"/>
    <dbReference type="STRING" id="266264.Rmet_3293"/>
    <dbReference type="EnsemblBacteria" id="ABF10165">
        <property type="protein sequence ID" value="ABF10165"/>
        <property type="gene ID" value="Rmet_3293"/>
    </dbReference>
    <dbReference type="GeneID" id="60826624"/>
    <dbReference type="KEGG" id="rme:Rmet_3293"/>
    <dbReference type="eggNOG" id="COG0100">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_072439_5_0_4"/>
    <dbReference type="OMA" id="KWGVAHI"/>
    <dbReference type="OrthoDB" id="1713391at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01310">
        <property type="entry name" value="Ribosomal_S11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001971">
        <property type="entry name" value="Ribosomal_S11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019981">
        <property type="entry name" value="Ribosomal_S11_bac-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018102">
        <property type="entry name" value="Ribosomal_S11_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036967">
        <property type="entry name" value="Ribosomal_S11_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11759">
        <property type="entry name" value="PTHR11759"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00411">
        <property type="entry name" value="Ribosomal_S11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002131">
        <property type="entry name" value="Ribosomal_S11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03632">
        <property type="entry name" value="uS11_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00054">
        <property type="entry name" value="RIBOSOMAL_S11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <feature type="chain" id="PRO_0000294832" description="30S ribosomal protein S11">
        <location>
            <begin position="1"/>
            <end position="133"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01310"/>
        </source>
    </evidence>
    <sequence length="133" mass="14077" checksum="BCBD7030E65EA796" modified="2006-05-30" version="1">MAKGPNNAAQRARKKVKKNVADGIAHVHASFNNTIITITDRQGNALSWATAGGQGFKGSRKSTPFAAQVAAENAGRVAQDQGIKNLEVRIKGPGPGRESAVRALNALGIKIAVIEDVTPVPHNGCRPPKRRRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LI86</accession>
    <name>Q1LI86_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Pili assembly protein PilQ</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">pilQ</name>
        <name evidence="9" type="ordered locus">Rmet_3268</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cell outer membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the bacterial secretin family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10140.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517740.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3268"/>
    <dbReference type="EnsemblBacteria" id="ABF10140">
        <property type="protein sequence ID" value="ABF10140"/>
        <property type="gene ID" value="Rmet_3268"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3268"/>
    <dbReference type="eggNOG" id="COG4796">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006756_0_2_4"/>
    <dbReference type="OMA" id="QNVPWDQ"/>
    <dbReference type="OrthoDB" id="870677at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009279">
        <property type="term" value="C:cell outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009306">
        <property type="term" value="P:protein secretion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021731">
        <property type="entry name" value="AMIN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001775">
        <property type="entry name" value="GspD/PilQ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005644">
        <property type="entry name" value="NolW-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038591">
        <property type="entry name" value="NolW-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013355">
        <property type="entry name" value="Pilus_4_PilQ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011662">
        <property type="entry name" value="Secretin/TonB_short_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004846">
        <property type="entry name" value="T2SS/T3SS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11741">
        <property type="entry name" value="AMIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00263">
        <property type="entry name" value="Secretin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03958">
        <property type="entry name" value="Secretin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07660">
        <property type="entry name" value="STN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00811">
        <property type="entry name" value="BCTERIALGSPD"/>
    </dbReference>
    <dbReference type="SMART" id="SM00965">
        <property type="entry name" value="STN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02515">
        <property type="entry name" value="IV_pilus_PilQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0998">Cell outer membrane</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0732">Signal</keyword>
    <keyword evidence="1 6" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193141" evidence="7">
        <location>
            <begin position="28"/>
            <end position="710"/>
        </location>
    </feature>
    <feature type="domain" description="STN" evidence="8">
        <location>
            <begin position="302"/>
            <end position="350"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU004003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU004004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00965"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ABF10140.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="710" mass="76510" checksum="CEFC0CB78AD9AAA7" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="107" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LI87</accession>
    <name>Q1LI87_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 10">Shikimate kinase</fullName>
            <shortName evidence="10">SK</shortName>
            <ecNumber evidence="3 10">2.7.1.71</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10 11" type="primary">aroK</name>
        <name evidence="11" type="ordered locus">Rmet_3267</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Catalyzes the specific phosphorylation of the 3-hydroxyl group of shikimic acid using ATP as a cosubstrate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>ATP + shikimate = 3-phosphoshikimate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13121"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:36208"/>
            <dbReference type="ChEBI" id="CHEBI:145989"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.71"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="10">Binds 1 Mg(2+) ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 10">Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 5/7.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the shikimate kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.71" evidence="3 10"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10139.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3267"/>
    <dbReference type="EnsemblBacteria" id="ABF10139">
        <property type="protein sequence ID" value="ABF10139"/>
        <property type="gene ID" value="Rmet_3267"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3267"/>
    <dbReference type="eggNOG" id="COG0703">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_057607_3_1_4"/>
    <dbReference type="OMA" id="FFCGIKH"/>
    <dbReference type="UniPathway" id="UPA00053">
        <property type="reaction ID" value="UER00088"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004765">
        <property type="term" value="F:shikimate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009073">
        <property type="term" value="P:aromatic amino acid family biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008652">
        <property type="term" value="P:cellular amino acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009423">
        <property type="term" value="P:chorismate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00464">
        <property type="entry name" value="SK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00109">
        <property type="entry name" value="Shikimate_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031322">
        <property type="entry name" value="Shikimate/glucono_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000623">
        <property type="entry name" value="Shikimate_kinase/TSH1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023000">
        <property type="entry name" value="Shikimate_kinase_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01202">
        <property type="entry name" value="SKI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01128">
        <property type="entry name" value="SHIKIMATE_KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 10" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="9 10" id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword evidence="8 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 10 11" id="KW-0418">Kinase</keyword>
    <keyword evidence="10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 10 11" id="KW-0808">Transferase</keyword>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="45"/>
            <end position="50"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="49"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="67"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="91"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="113"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="151"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="170"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ABF10139.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="229" mass="25063" checksum="086912741E96BDC5" modified="2006-05-30" version="1">MMHNGVCITPLAGSRPDAGARRLIVQDLSPVTANRPNIFFVGLMGAGKTTVGRTVARRLNYPFFDSDHELEARCGVRIPIIFEHEGEAGFRDREAQVIAELTGRSGIVLATGGGAVLREVNRAALKANGTVVYLRASPHDLWLRTRHDRNRPLLQTEDPKGRLEALYAERDPLYREVADFIIETGKPTVAQLANMVLMQLEMAGFVFPPAEIVPESAEDGGPPAQDTHS</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="69" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LIA9</accession>
    <name>Q1LIA9_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">UPF0056 inner membrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Rmet_3245</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cell inner membrane</location>
            <topology evidence="7">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the UPF0056 (MarC) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10117.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008643049.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3245"/>
    <dbReference type="EnsemblBacteria" id="ABF10117">
        <property type="protein sequence ID" value="ABF10117"/>
        <property type="gene ID" value="Rmet_3245"/>
    </dbReference>
    <dbReference type="GeneID" id="60825569"/>
    <dbReference type="KEGG" id="rme:Rmet_3245"/>
    <dbReference type="eggNOG" id="COG2095">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_079909_2_1_4"/>
    <dbReference type="OMA" id="LFVIMDP"/>
    <dbReference type="OrthoDB" id="1323133at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002771">
        <property type="entry name" value="Multi_antbiot-R_MarC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33508">
        <property type="entry name" value="PTHR33508"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01914">
        <property type="entry name" value="MarC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00427">
        <property type="entry name" value="TIGR00427"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="38"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="63"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="108"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="139"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="181"/>
            <end position="202"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009784"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU362048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ABF10117.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="207" mass="21805" checksum="59FEBF7B7A038880" modified="2006-05-30" version="1">MDTLKSFISLLALINPIGAIPFFISLTSQQTEAEKLRTIKTASVSVAIVVGVSALLGQQIIEFFNISVASLEVGGGLLMVMMAMNMLNAQTSRTKATPEEENEAEAKASIAVVPLALPLLTGPGSISTVIVYAGKTQHWYQLLILVGIGVLLGAVVYVTFRAADPIARVIGQTGINIGTRLMGLILSALAVEFIVDGLKTLLPVLKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LIE9</accession>
    <name>Q1LIE9_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Flavin prenyltransferase UbiX</fullName>
            <ecNumber evidence="5">2.5.1.129</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5 7" type="primary">ubiX</name>
        <name evidence="7" type="ordered locus">Rmet_3205</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Flavin prenyltransferase that catalyzes the synthesis of the prenylated FMN cofactor (prenyl-FMN) for 4-hydroxy-3-polyprenylbenzoic acid decarboxylase UbiD. The prenyltransferase is metal-independent and links a dimethylallyl moiety from dimethylallyl monophosphate (DMAP) to the flavin N5 and C6 atoms of FMN.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>dimethylallyl phosphate + FMNH2 = phosphate + prenyl-FMNH2</text>
            <dbReference type="Rhea" id="RHEA:37743"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57618"/>
            <dbReference type="ChEBI" id="CHEBI:87467"/>
            <dbReference type="ChEBI" id="CHEBI:88052"/>
            <dbReference type="EC" id="2.5.1.129"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the UbiX/PAD1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.129" evidence="5"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10077.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517686.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3205"/>
    <dbReference type="EnsemblBacteria" id="ABF10077">
        <property type="protein sequence ID" value="ABF10077"/>
        <property type="gene ID" value="Rmet_3205"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3205"/>
    <dbReference type="eggNOG" id="COG0163">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_074522_0_1_4"/>
    <dbReference type="OMA" id="GATHIQD"/>
    <dbReference type="OrthoDB" id="1384786at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106141">
        <property type="term" value="F:flavin prenyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1950">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01984">
        <property type="entry name" value="ubiX_pad"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036551">
        <property type="entry name" value="Flavin_trans-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003382">
        <property type="entry name" value="Flavoprotein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004507">
        <property type="entry name" value="UbiX-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43374">
        <property type="entry name" value="PTHR43374"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02441">
        <property type="entry name" value="Flavoprotein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52507">
        <property type="entry name" value="SSF52507"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00421">
        <property type="entry name" value="ubiX_pad"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 5" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="3 5" id="KW-0288">FMN</keyword>
    <keyword evidence="7" id="KW-0456">Lyase</keyword>
    <keyword evidence="1 5" id="KW-0637">Prenyltransferase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Flavoprotein" evidence="6">
        <location>
            <begin position="11"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="18"/>
            <end position="20"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="45"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="96"/>
            <end position="99"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="131"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="161"/>
        </location>
        <ligand>
            <name>dimethylallyl phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:88052"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="177"/>
        </location>
        <ligand>
            <name>dimethylallyl phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:88052"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022602"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF02441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABF10077.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="204" mass="21857" checksum="5BEB76BE0F503951" modified="2006-05-30" version="1">MPVTNGRAPQRLIIAITGATGAIYGVRLLQALQGVADVETHLLMSPAGVMNLQHELDMGRAEVEALADVVHNVRDIGATIASGSFRANAMIVAPCSMRTLAAIAHGLSDNLITRAADVTLKERRKLVLMVRETPLNLAHLRNMTAVTEMGGIVFPPVPGFYQKPQTIDELVDHTVGRVLDLVDLREIGEKLAPGWGGLNNSAED</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LIT2</accession>
    <name>Q1LIT2_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_3072</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09944.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3072"/>
    <dbReference type="EnsemblBacteria" id="ABF09944">
        <property type="protein sequence ID" value="ABF09944"/>
        <property type="gene ID" value="Rmet_3072"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3072"/>
    <dbReference type="HOGENOM" id="CLU_159845_0_0_4"/>
    <dbReference type="OMA" id="RCGLIEY"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF09944.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="144" mass="15875" checksum="98B4FCF6334DA91C" modified="2006-05-30" version="1">MTQTEPKWLFKTRAVSSCPVTVWASYPRMIAVRSARGHARTVDLLTDISSFQAARRHDGRCGQIEFSRARLSPGIRLSAGFAARSRMPGRRGHTAMSAQVRAGRGCLPATEFISVRSMLRRAPMNRSLDWGTCKTRLGSVGSPI</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LIV6</accession>
    <name>Q1LIV6_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Phosphatidylglycerophosphatase A</fullName>
            <ecNumber evidence="1">3.1.3.27</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Phosphatidylglycerolphosphate phosphatase A</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="4" type="primary">pgpA</name>
        <name evidence="4" type="ordered locus">Rmet_3048</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Lipid phosphatase which dephosphorylates phosphatidylglycerophosphate (PGP) to phosphatidylglycerol (PG).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>1,2-diacyl-sn-glycero-3-phospho-(1'-sn-glycero-3'-phosphate) + H2O = 1,2-diacyl-sn-glycero-3-phospho-(1'-sn-glycerol) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:33751"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:60110"/>
            <dbReference type="ChEBI" id="CHEBI:64716"/>
            <dbReference type="EC" id="3.1.3.27"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="1">Phospholipid metabolism; phosphatidylglycerol biosynthesis; phosphatidylglycerol from CDP-diacylglycerol: step 2/2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="3.1.3.27" evidence="1"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09920.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517551.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3048"/>
    <dbReference type="EnsemblBacteria" id="ABF09920">
        <property type="protein sequence ID" value="ABF09920"/>
        <property type="gene ID" value="Rmet_3048"/>
    </dbReference>
    <dbReference type="GeneID" id="60820574"/>
    <dbReference type="KEGG" id="rme:Rmet_3048"/>
    <dbReference type="eggNOG" id="COG1267">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_103734_0_0_4"/>
    <dbReference type="OMA" id="VWDEIAG"/>
    <dbReference type="OrthoDB" id="1969080at2"/>
    <dbReference type="UniPathway" id="UPA00084">
        <property type="reaction ID" value="UER00504"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008962">
        <property type="term" value="F:phosphatidylglycerophosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006655">
        <property type="term" value="P:phosphatidylglycerol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009395">
        <property type="term" value="P:phospholipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06971">
        <property type="entry name" value="PgpA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026037">
        <property type="entry name" value="PgpA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036681">
        <property type="entry name" value="PgpA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007686">
        <property type="entry name" value="YutG/PgpA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36305">
        <property type="entry name" value="PTHR36305"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04608">
        <property type="entry name" value="PgpA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006162">
        <property type="entry name" value="PgpA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101307">
        <property type="entry name" value="SSF101307"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="1" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="1 4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="1" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="1" id="KW-0460">Magnesium</keyword>
    <keyword evidence="1 2" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1" id="KW-0595">Phospholipid degradation</keyword>
    <keyword evidence="1" id="KW-1208">Phospholipid metabolism</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="42"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="79"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="117"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="162"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="domain" description="PgpA" evidence="3">
        <location>
            <begin position="41"/>
            <end position="187"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRNR" id="PIRNR006162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF04608"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF09920.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="190" mass="21010" checksum="04BED32CD7A9602F" modified="2006-05-30" version="1">MSAYPPGAAPSDPVDPVTLEAGQTAKVTRPTGRFMLGHPARLIALGFGSGLSPVSPGTVGTLLGWLSFIVISKWIDPTTWLWIIAGGFVIGIWACLRTARDMGVYDHGSMVWDEIVAFWLVLLFVMPTGFWGQFAAFVWFRFFDIVKPAPIRYYDRTLKGPGFTGAFGVMFDDIFAAFYTLLVFALWRSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LJ17</accession>
    <name>Q1LJ17_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Guanyl-specific exported ribonuclease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Rmet_2986</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ribonuclease N1/T1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09859.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2986"/>
    <dbReference type="EnsemblBacteria" id="ABF09859">
        <property type="protein sequence ID" value="ABF09859"/>
        <property type="gene ID" value="Rmet_2986"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2986"/>
    <dbReference type="eggNOG" id="COG4290">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_112496_2_0_4"/>
    <dbReference type="OMA" id="AANDCYY"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004521">
        <property type="term" value="F:endoribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000026">
        <property type="entry name" value="Gua-sp_ribonuclease_N1/T1/U2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016191">
        <property type="entry name" value="Ribonuclease/ribotoxin"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00545">
        <property type="entry name" value="Ribonuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53933">
        <property type="entry name" value="SSF53933"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0540">Nuclease</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF09859.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="147" mass="16299" checksum="94F72B22EEBC1047" modified="2006-05-30" version="1">MSSQSQRQSHWQSLSLARSLGHVLAGAAISIALTLAPQGAMARQSPDDVQGVVGTIAAQQLPNEARQTLERIEAGGPFPYDKDGSRFGNYERSLPQQSRGYYREYTVKNSNSRNRGAKRIVCGGDQRAANDCYYTEDHYNSFKRITK</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="69" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LJ50</accession>
    <name>Q1LJ50_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Polyprenyl-pyrophosphate binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">yceI</name>
        <name evidence="3" type="ordered locus">Rmet_2953</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09826.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517493.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2953"/>
    <dbReference type="EnsemblBacteria" id="ABF09826">
        <property type="protein sequence ID" value="ABF09826"/>
        <property type="gene ID" value="Rmet_2953"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2953"/>
    <dbReference type="eggNOG" id="COG2353">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_071003_1_2_4"/>
    <dbReference type="OMA" id="FKCMQHP"/>
    <dbReference type="OrthoDB" id="1767227at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.128.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007372">
        <property type="entry name" value="Lipid/polyisoprenoid-bd_YceI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036761">
        <property type="entry name" value="TTHA0802/YceI-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04264">
        <property type="entry name" value="YceI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00867">
        <property type="entry name" value="YceI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101874">
        <property type="entry name" value="SSF101874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193511" evidence="1">
        <location>
            <begin position="25"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="domain" description="YceI" evidence="2">
        <location>
            <begin position="28"/>
            <end position="193"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00867"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF09826.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="196" mass="21096" checksum="1EC81DFEEC2DCE0D" modified="2006-05-30" version="1">MKMRTLFAAVAAASATLAAGTALANASTYNIDPTHTYPSFEADHMGGLSTWRGKFEKSSGVVTLDRAGKTGSVDIKIDASSIDFGNGKLNEHAKGPEMFDVQAFPEATYKGKFSKFKGDVPTEVDGVLTLHGVSKPVKLEIREFKCMQHPMLKREACGADAVAQINRADFGIDYGVKYGFKQDVKLAIQVEAVKAD</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2007-07-24" modified="2022-08-03" version="96" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LJ86</accession>
    <name>PANB_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">3-methyl-2-oxobutanoate hydroxymethyltransferase</fullName>
            <ecNumber evidence="1">2.1.2.11</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Ketopantoate hydroxymethyltransferase</fullName>
            <shortName evidence="1">KPHMT</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">panB</name>
        <name type="ordered locus">Rmet_2917</name>
    </gene>
    <organism>
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the reversible reaction in which hydroxymethyl group from 5,10-methylenetetrahydrofolate is transferred onto alpha-ketoisovalerate to form ketopantoate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(6R)-5,10-methylene-5,6,7,8-tetrahydrofolate + 3-methyl-2-oxobutanoate + H2O = (6S)-5,6,7,8-tetrahydrofolate + 2-dehydropantoate</text>
            <dbReference type="Rhea" id="RHEA:11824"/>
            <dbReference type="ChEBI" id="CHEBI:11561"/>
            <dbReference type="ChEBI" id="CHEBI:11851"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15636"/>
            <dbReference type="ChEBI" id="CHEBI:57453"/>
            <dbReference type="EC" id="2.1.2.11"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="1">Binds 1 Mg(2+) ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; (R)-pantothenate biosynthesis; (R)-pantoate from 3-methyl-2-oxobutanoate: step 1/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodecamer; pentamer of dimers.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the PanB family.</text>
    </comment>
    <dbReference type="EC" id="2.1.2.11" evidence="1"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09790.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517463.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q1LJ86"/>
    <dbReference type="SMR" id="Q1LJ86"/>
    <dbReference type="STRING" id="266264.Rmet_2917"/>
    <dbReference type="EnsemblBacteria" id="ABF09790">
        <property type="protein sequence ID" value="ABF09790"/>
        <property type="gene ID" value="Rmet_2917"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2917"/>
    <dbReference type="eggNOG" id="COG0413">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036645_1_0_4"/>
    <dbReference type="OMA" id="VLVWTDM"/>
    <dbReference type="OrthoDB" id="916845at2"/>
    <dbReference type="UniPathway" id="UPA00028">
        <property type="reaction ID" value="UER00003"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003864">
        <property type="term" value="F:3-methyl-2-oxobutanoate hydroxymethyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015940">
        <property type="term" value="P:pantothenate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd06557">
        <property type="entry name" value="KPHMT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00156">
        <property type="entry name" value="PanB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003700">
        <property type="entry name" value="Pantoate_hydroxy_MeTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015813">
        <property type="entry name" value="Pyrv/PenolPyrv_Kinase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040442">
        <property type="entry name" value="Pyrv_Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20881">
        <property type="entry name" value="PTHR20881"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02548">
        <property type="entry name" value="Pantoate_transf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000388">
        <property type="entry name" value="Pantoate_hydroxy_MeTrfase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51621">
        <property type="entry name" value="SSF51621"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00222">
        <property type="entry name" value="panB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0566">Pantothenate biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000297348" description="3-methyl-2-oxobutanoate hydroxymethyltransferase">
        <location>
            <begin position="1"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="1">
        <location>
            <position position="189"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="53"/>
            <end position="54"/>
        </location>
        <ligand>
            <name>3-methyl-2-oxobutanoate</name>
            <dbReference type="ChEBI" id="CHEBI:11851"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="53"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="92"/>
        </location>
        <ligand>
            <name>3-methyl-2-oxobutanoate</name>
            <dbReference type="ChEBI" id="CHEBI:11851"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="92"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="120"/>
        </location>
        <ligand>
            <name>3-methyl-2-oxobutanoate</name>
            <dbReference type="ChEBI" id="CHEBI:11851"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="122"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00156"/>
        </source>
    </evidence>
    <sequence length="273" mass="29018" checksum="19D9CD80A38B498F" modified="2006-05-30" version="1">MSYLLDPSRKTVTIPKLQAMREAGEKIAMLTAYDSSFSALLDLCGVEVILVGDSLGNVMQGQQTTLPVTLEHMVYHTECVSRGNQTALLVSDLPFGTYPTPEKAFESAAALMRAGAQMVKLEGGDWLAPTVKFLVERSIPVCAHIGLTPQSVHALGGFKVQGKTDESAAQLKRDALALQQAGAQLIVMEAVPAKLAAEVTAQLSIPTIGIGAGVDCSGQVLVLQDMINIYPGRKAKFVRNFMEGQPSIEAAIRAYVAAVKDGSFPAVEHTFSA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-01-15" modified="2022-08-03" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LJD2</accession>
    <name>RL33_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">50S ribosomal protein L33</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpmG</name>
        <name type="ordered locus">Rmet_2871</name>
    </gene>
    <organism>
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the bacterial ribosomal protein bL33 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09744.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008644909.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q1LJD2"/>
    <dbReference type="SMR" id="Q1LJD2"/>
    <dbReference type="STRING" id="266264.Rmet_2871"/>
    <dbReference type="EnsemblBacteria" id="ABF09744">
        <property type="protein sequence ID" value="ABF09744"/>
        <property type="gene ID" value="Rmet_2871"/>
    </dbReference>
    <dbReference type="GeneID" id="60820690"/>
    <dbReference type="KEGG" id="rme:Rmet_2871"/>
    <dbReference type="eggNOG" id="COG0267">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_190949_1_1_4"/>
    <dbReference type="OMA" id="GTGFYYT"/>
    <dbReference type="OrthoDB" id="2034291at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.28.120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00294">
        <property type="entry name" value="Ribosomal_L33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038584">
        <property type="entry name" value="L33_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001705">
        <property type="entry name" value="Ribosomal_L33"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018264">
        <property type="entry name" value="Ribosomal_L33_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011332">
        <property type="entry name" value="Ribosomal_zn-bd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00471">
        <property type="entry name" value="Ribosomal_L33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57829">
        <property type="entry name" value="SSF57829"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01023">
        <property type="entry name" value="rpmG_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00582">
        <property type="entry name" value="RIBOSOMAL_L33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <feature type="chain" id="PRO_1000004183" description="50S ribosomal protein L33">
        <location>
            <begin position="1"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="56" mass="6398" checksum="82B0556D727138E5" modified="2006-05-30" version="1">MASKGGRDKIKLESTAGTGHFYTTTKNKRTMPEKMEISKFDPVARKHVPYKETKIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-10-07" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LJK4</accession>
    <name>Q1LJK4_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_2799</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09672.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2799"/>
    <dbReference type="EnsemblBacteria" id="ABF09672">
        <property type="protein sequence ID" value="ABF09672"/>
        <property type="gene ID" value="Rmet_2799"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2799"/>
    <dbReference type="HOGENOM" id="CLU_2261483_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="26"/>
            <end position="50"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF09672.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="103" mass="11597" checksum="AB8D9D403766C2D6" modified="2006-05-30" version="1">MRPKTTEKPNGKICTHAWVLERGQALGQRQHVKSDPRAPGASSPASMMLSSSGSFWRGQFSCGRQERCPPHLYPLYPHPIVVYRPHLTGLSDITVWRMADSRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="64" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LJU6</accession>
    <name>Q1LJU6_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_2707</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09580.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517279.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2707"/>
    <dbReference type="EnsemblBacteria" id="ABF09580">
        <property type="protein sequence ID" value="ABF09580"/>
        <property type="gene ID" value="Rmet_2707"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2707"/>
    <dbReference type="eggNOG" id="ENOG5032S46">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_148512_0_1_4"/>
    <dbReference type="OMA" id="SAEMCGD"/>
    <dbReference type="OrthoDB" id="1677662at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF09580.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="99" mass="10266" checksum="1B1938205502CF5B" modified="2006-05-30" version="1">MTCQSALTCREGCGACCIAPSITSPLPGMPAGKAAGQACAQLLPDMRCAVFGRPERPDFCGGLRPSHEMCGESREAALLWLTRLEAMTAPASRSDGGHA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LJY3</accession>
    <name>Q1LJY3_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Methyltransferase</fullName>
            <ecNumber evidence="6">2.1.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Rmet_2670</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a 2'-deoxycytidine in DNA + S-adenosyl-L-methionine = an N(4)-methyl-2'-deoxycytidine in DNA + H(+) + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:16857"/>
            <dbReference type="Rhea" id="RHEA-COMP:11369"/>
            <dbReference type="Rhea" id="RHEA-COMP:13674"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:85452"/>
            <dbReference type="ChEBI" id="CHEBI:137933"/>
            <dbReference type="EC" id="2.1.1.113"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the N(4)/N(6)-methyltransferase family. N(4) subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.-" evidence="6"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09543.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2670"/>
    <dbReference type="REBASE" id="12356">
        <property type="entry name" value="M.RmeAORF2670P"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF09543">
        <property type="protein sequence ID" value="ABF09543"/>
        <property type="gene ID" value="Rmet_2670"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2670"/>
    <dbReference type="eggNOG" id="COG2189">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024927_2_0_4"/>
    <dbReference type="OMA" id="RHEYIIH"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008170">
        <property type="term" value="F:N-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015667">
        <property type="term" value="F:site-specific DNA-methyltransferase (cytosine-N4-specific) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002941">
        <property type="entry name" value="DNA_methylase_N4/N6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017985">
        <property type="entry name" value="MeTrfase_CN4_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001091">
        <property type="entry name" value="RM_Methyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01555">
        <property type="entry name" value="N6_N4_Mtase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00508">
        <property type="entry name" value="S21N4MTFRASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00093">
        <property type="entry name" value="N4_MTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 8" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N6_N4_Mtase" evidence="7">
        <location>
            <begin position="36"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="domain" description="N6_N4_Mtase" evidence="7">
        <location>
            <begin position="317"/>
            <end position="347"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU362026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ABF09543.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="388" mass="42561" checksum="310DD22EC3FCFE51" modified="2006-05-30" version="1">MMTTKPESSVFSNVSAPELLRYHSDARHIPVPDASVDLIVTSPPYWKKRDYGFDGQIGQESTPAAYVANMIECMREWRRVLKPSGSIFLNVGDSYMARTLAGVPGRLEAAAIDDGWLVRNRIIWAKESGMPEPAKNRLANRHEYIIHFVSSAKYFYDLAGYAEANGNGANPGDVWNIPLRRNMGDHLAPFPNEIVRRAILLACPIATCPKCGHIPERVVQRTAVLDESRPQARRAMEIAREAGLTDAHIAAVQATGISDAGKALRVQTGTGRNSREVQRLAAEAKEVLGGYFREFTFAKKQTVGWTTCSCKGKLKPGVVLDPFMGTGTTLSTALEMGRSAIGVDLAPKTDDHVWGADAESLENVDRAVKPVSVKRRSRSDSSRLALQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LK88</accession>
    <name>Q1LK88_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Tyrosine-based site-specific recombinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">int</name>
        <name evidence="7" type="ordered locus">Rmet_2561</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09438.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517141.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2561"/>
    <dbReference type="EnsemblBacteria" id="ABF09438">
        <property type="protein sequence ID" value="ABF09438"/>
        <property type="gene ID" value="Rmet_2561"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2561"/>
    <dbReference type="eggNOG" id="COG0582">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_043523_3_0_4"/>
    <dbReference type="OMA" id="PEGFPRY"/>
    <dbReference type="OrthoDB" id="785253at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.443.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044068">
        <property type="entry name" value="CB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011010">
        <property type="entry name" value="DNA_brk_join_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013762">
        <property type="entry name" value="Integrase-like_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002104">
        <property type="entry name" value="Integrase_catalytic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010998">
        <property type="entry name" value="Integrase_recombinase_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00589">
        <property type="entry name" value="Phage_integrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56349">
        <property type="entry name" value="SSF56349"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51900">
        <property type="entry name" value="CB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51898">
        <property type="entry name" value="TYR_RECOMBINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0229">DNA integration</keyword>
    <keyword evidence="3" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="2 4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Core-binding (CB)" evidence="6">
        <location>
            <begin position="155"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="domain" description="Tyr recombinase" evidence="5">
        <location>
            <begin position="260"/>
            <end position="458"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABF09438.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="458" mass="51200" checksum="728EA3DB5D922832" modified="2006-05-30" version="1">MKLTLGVLRVTLKHAFLRGGVIYYQRAIPKDLQDRYSAKLVKASLETGDVLVAKRKIEQLNRELEAEWAMMRGHPEATPKTIQGQAVELLRKWGLTATPTDDDEDAIDLFRESLDEKRQRYAEGDDEVYSDADGSEYLSPVEIKAAQIIAGTIKPVLSEALELYLKVHPKRNDKRFEDGARLVFKTLTDAIGDKPIEDLSRSDAHKYVAAEIARGVKTGTVNRYMNTIRAIIGTWLREKEIAKANPFTRIPIPENGKDAKKREPFTEPELKTLFAACKTKDDDVRWLTAILGDTGARLAEIAGLALDDINLDAEIPHVVIQPHPWRSLKNDVSARTVPLLGHALWAAQRIKETARKGQRFAFPRYTSADKAGTAVETKATHASNTIAKWIRTLKVDGVKMNHTAHELRHTMADRLREVGCPPEIRLAIGGWAIDGIGATYGKGYTLRVMSEWLGKVTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LKC3</accession>
    <name>Q1LKC3_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">3-Oxoacyl-(Acyl-carrier-protein) reductase</fullName>
            <ecNumber evidence="1">1.1.1.100</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">fabG</name>
        <name evidence="1" type="ordered locus">Rmet_2526</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EC" id="1.1.1.100" evidence="1"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09403.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2526"/>
    <dbReference type="EnsemblBacteria" id="ABF09403">
        <property type="protein sequence ID" value="ABF09403"/>
        <property type="gene ID" value="Rmet_2526"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2526"/>
    <dbReference type="eggNOG" id="COG1028">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010194_1_3_4"/>
    <dbReference type="OMA" id="REDGMER"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004316">
        <property type="term" value="F:3-oxoacyl-[acyl-carrier-protein] reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00061">
        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF09403.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="289" mass="30093" checksum="6B161B8638990874" modified="2006-05-30" version="1">MAPASSIERNHAMSKLAGKSAIVTGSGRGIGREVAMKLAAEGARVVVNDLDADPAQEVVELIRANGGEAVACVGSVTAPDFADRLIKTTVDSFKGIDIIVNNAGYTWDNVIQKMTDEQWYAILDCHLTAPFRILRAAQPVISAAAKAEAAEGREVFRKVVNISSGAAGGNAGQSNYSAAKAGILGMTKTLAKEWGRLKVNVNAVAFGLIETRLTQALAADQSKSVSIEGREIKVGVQQAMLDGASRMIPVGRAGRAEEAAGAVYLLCTPESNFLSGQVLYCGGGPGANF</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="59" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LKU0</accession>
    <name>Q1LKU0_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative membrane protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_2359</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09236.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_003149767.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2359"/>
    <dbReference type="EnsemblBacteria" id="ABF09236">
        <property type="protein sequence ID" value="ABF09236"/>
        <property type="gene ID" value="Rmet_2359"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2359"/>
    <dbReference type="eggNOG" id="ENOG502ZVEC">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_163355_0_0_4"/>
    <dbReference type="OMA" id="GYFFVWR"/>
    <dbReference type="OrthoDB" id="1999007at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="22"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="57"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="97"/>
            <end position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF09236.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="118" mass="13272" checksum="E4225FDAB8141DF2" modified="2006-05-30" version="1">MSAARMTWRPLRWLFSRRAAKALLWTVLIVAAAVAANIAGIYLVGSVAGWERWLAAAAGYLFVWRLCLYGATVYGWIWMRRRLLVREDDAQARHRLVRTEIAGVVAIVALEASLLMQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="68" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LKU6</accession>
    <name>Q1LKU6_CUPMC</name>
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        <submittedName>
            <fullName evidence="2">Type IV secretory pathway, VirB4 components</fullName>
        </submittedName>
    </protein>
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        <name evidence="2" type="ordered locus">Rmet_2353</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
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        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
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                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="TraC_F_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03744">
        <property type="entry name" value="traC_PFL_4706"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF09230.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="963" mass="108066" checksum="4AD2E1DCD9266DBD" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="55" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LKW0</accession>
    <name>Q1LKW0_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_2339</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
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        <lineage>
            <taxon>Bacteria</taxon>
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            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09216.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_003050273.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="SMR" id="Q1LKW0"/>
    <dbReference type="STRING" id="266264.Rmet_2339"/>
    <dbReference type="EnsemblBacteria" id="ABF09216">
        <property type="protein sequence ID" value="ABF09216"/>
        <property type="gene ID" value="Rmet_2339"/>
    </dbReference>
    <dbReference type="GeneID" id="56409846"/>
    <dbReference type="KEGG" id="rme:Rmet_2339"/>
    <dbReference type="eggNOG" id="ENOG502Z7SP">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_078737_0_0_4"/>
    <dbReference type="OMA" id="GGTYPPK"/>
    <dbReference type="OrthoDB" id="1472532at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF09216.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="241" mass="26663" checksum="0D42F5846786EEEA" modified="2006-05-30" version="1">MSQWSLSQLLSSLHEDIQQRLSVVRKTFGHPGTKGDASENVWIDMLDTYLPKRYQAAKAHVVDSLGNFSQQIDVVVFDRQYSPFIFTYENETIIPAESVYAVFEAKQTADAGLVAYAQEKVASVRRLHRTSLPIPHAGGTYPAKPLIPILGGLLTFESEWSPALGPSMDKALNANLTEGRLDIGCVAAHGHFFYDQASGAYSYTNENKPATAFLFKLIAQLQFSGTVPMIDVEAYGQWLTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LL94</accession>
    <name>Q1LL94_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Propionyl-CoA synthetase</fullName>
            <ecNumber evidence="4">6.2.1.17</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">prpE</name>
        <name evidence="4" type="ordered locus">Rmet_2203</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EC" id="6.2.1.17" evidence="4"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09082.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516910.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2203"/>
    <dbReference type="EnsemblBacteria" id="ABF09082">
        <property type="protein sequence ID" value="ABF09082"/>
        <property type="gene ID" value="Rmet_2203"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2203"/>
    <dbReference type="eggNOG" id="COG0365">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000022_3_6_4"/>
    <dbReference type="OMA" id="YVFVMGR"/>
    <dbReference type="OrthoDB" id="141801at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050218">
        <property type="term" value="F:propionate-CoA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019629">
        <property type="term" value="P:propionate catabolic process, 2-methylcitrate cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032387">
        <property type="entry name" value="ACAS_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025110">
        <property type="entry name" value="AMP-bd_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045851">
        <property type="entry name" value="AMP-bd_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020845">
        <property type="entry name" value="AMP-binding_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_Synth/Lig"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012694">
        <property type="entry name" value="Propion_PrpE"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16177">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02316">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00455">
        <property type="entry name" value="AMP_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="5"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="domain" description="AMP-binding" evidence="1">
        <location>
            <begin position="62"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="domain" description="AMP-binding_C" evidence="2">
        <location>
            <begin position="511"/>
            <end position="594"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF16177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF09082.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="629" mass="68869" checksum="342AD5864B1C733C" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-12-12" modified="2022-08-03" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LLI7</accession>
    <name>NDK_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Nucleoside diphosphate kinase</fullName>
            <shortName evidence="1">NDK</shortName>
            <shortName evidence="1">NDP kinase</shortName>
            <ecNumber evidence="1">2.7.4.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Nucleoside-2-P kinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">ndk</name>
        <name type="ordered locus">Rmet_2110</name>
    </gene>
    <organism>
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Major role in the synthesis of nucleoside triphosphates other than ATP. The ATP gamma phosphate is transferred to the NDP beta phosphate via a ping-pong mechanism, using a phosphorylated active-site intermediate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a 2'-deoxyribonucleoside 5'-diphosphate + ATP = a 2'-deoxyribonucleoside 5'-triphosphate + ADP</text>
            <dbReference type="Rhea" id="RHEA:44640"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61560"/>
            <dbReference type="ChEBI" id="CHEBI:73316"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.6"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a ribonucleoside 5'-diphosphate + ATP = a ribonucleoside 5'-triphosphate + ADP</text>
            <dbReference type="Rhea" id="RHEA:18113"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57930"/>
            <dbReference type="ChEBI" id="CHEBI:61557"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.6"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homotetramer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the NDK family.</text>
    </comment>
    <dbReference type="EC" id="2.7.4.6" evidence="1"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08989.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516823.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q1LLI7"/>
    <dbReference type="SMR" id="Q1LLI7"/>
    <dbReference type="STRING" id="266264.Rmet_2110"/>
    <dbReference type="EnsemblBacteria" id="ABF08989">
        <property type="protein sequence ID" value="ABF08989"/>
        <property type="gene ID" value="Rmet_2110"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2110"/>
    <dbReference type="eggNOG" id="COG0105">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_060216_8_1_4"/>
    <dbReference type="OMA" id="KIVAMKM"/>
    <dbReference type="OrthoDB" id="1752581at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004550">
        <property type="term" value="F:nucleoside diphosphate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006241">
        <property type="term" value="P:CTP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006183">
        <property type="term" value="P:GTP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006165">
        <property type="term" value="P:nucleoside diphosphate phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006228">
        <property type="term" value="P:UTP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.141">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00451">
        <property type="entry name" value="NDP_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034907">
        <property type="entry name" value="NDK-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036850">
        <property type="entry name" value="NDK-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001564">
        <property type="entry name" value="Nucleoside_diP_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023005">
        <property type="entry name" value="Nucleoside_diP_kinase_AS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00334">
        <property type="entry name" value="NDK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01243">
        <property type="entry name" value="NUCDPKINASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00562">
        <property type="entry name" value="NDK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54919">
        <property type="entry name" value="SSF54919"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00469">
        <property type="entry name" value="NDP_KINASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0418">Kinase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0546">Nucleotide metabolism</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0597">Phosphoprotein</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000267794" description="Nucleoside diphosphate kinase">
        <location>
            <begin position="1"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="active site" description="Pros-phosphohistidine intermediate" evidence="1">
        <location>
            <position position="117"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="11"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="59"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="87"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="93"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="104"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="114"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00451"/>
        </source>
    </evidence>
    <sequence length="141" mass="15247" checksum="56CCBD25085356A4" modified="2006-05-30" version="1">MAIERTLSIIKPDAVAKNVIGQIYARFEAAGLKVVAAKMVHLSRGEAEQFYAVHKARPFFKDLVDFMVSGPVMIQALEGESAIAKNRDLMGATDPKKAEKGTIRADFADSIDANAVHGSDAPETAAVEVAFFFPGMNVYSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LLN3</accession>
    <name>Q1LLN3_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative competence-damage protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_2064</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08943.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516777.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2064"/>
    <dbReference type="EnsemblBacteria" id="ABF08943">
        <property type="protein sequence ID" value="ABF08943"/>
        <property type="gene ID" value="Rmet_2064"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2064"/>
    <dbReference type="eggNOG" id="COG1058">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030805_0_1_4"/>
    <dbReference type="OMA" id="MAWPMME"/>
    <dbReference type="OrthoDB" id="1834406at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd00885">
        <property type="entry name" value="cinA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.980.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036425">
        <property type="entry name" value="MoaB/Mog-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001453">
        <property type="entry name" value="MoaB/Mog_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00994">
        <property type="entry name" value="MoCF_biosynth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00852">
        <property type="entry name" value="MoCF_biosynth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53218">
        <property type="entry name" value="SSF53218"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MoCF_biosynth" evidence="1">
        <location>
            <begin position="4"/>
            <end position="177"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF08943.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="266" mass="29382" checksum="D1C2684D559B3066" modified="2006-05-30" version="1">MGFGLIVIGDEILSGRREDKHMRRAIELLGARGLALDWAEYIGDDRERITATLRRTLAGPDIVFCTGGIGATPDDHTRQCVAAALGVDLALHPEARELIALRIAETANGDPVKADLNQPENQHRFKMGEFPVGARIIPNSYNRIPGFSVADHHFMPGFPVMAWPMMEWVLDNRYAHLFHQDTEVSRAFYVFEAPESTLTPLMEQVEASFAGIRVFSLPSVGDAARGDKFVRRHIDLGVKGPADMVGPAYAMLLDGVRAMGFEIVEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LLW7</accession>
    <name>Q1LLW7_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Periplasmic L-asparaginase II</fullName>
            <ecNumber evidence="10">3.5.1.1</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">ansB</name>
        <name evidence="10" type="ordered locus">Rmet_1980</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the asparaginase 1 family.</text>
    </comment>
    <dbReference type="EC" id="3.5.1.1" evidence="10"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08859.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516700.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1980"/>
    <dbReference type="EnsemblBacteria" id="ABF08859">
        <property type="protein sequence ID" value="ABF08859"/>
        <property type="gene ID" value="Rmet_1980"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1980"/>
    <dbReference type="eggNOG" id="COG0252">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019134_1_2_4"/>
    <dbReference type="OMA" id="LTCAMRP"/>
    <dbReference type="OrthoDB" id="1659551at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0110165">
        <property type="term" value="C:cellular anatomical entity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004067">
        <property type="term" value="F:asparaginase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006528">
        <property type="term" value="P:asparagine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd08964">
        <property type="entry name" value="L-asparaginase_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004550">
        <property type="entry name" value="AsnASE_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036152">
        <property type="entry name" value="Asp/glu_Ase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006034">
        <property type="entry name" value="Asparaginase/glutaminase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020827">
        <property type="entry name" value="Asparaginase/glutaminase_AS1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027475">
        <property type="entry name" value="Asparaginase/glutaminase_AS2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040919">
        <property type="entry name" value="Asparaginase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027473">
        <property type="entry name" value="L-asparaginase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027474">
        <property type="entry name" value="L-asparaginase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037152">
        <property type="entry name" value="L-asparaginase_N_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00710">
        <property type="entry name" value="Asparaginase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17763">
        <property type="entry name" value="Asparaginase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001220">
        <property type="entry name" value="L-ASNase_gatD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00139">
        <property type="entry name" value="ASNGLNASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00870">
        <property type="entry name" value="Asparaginase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53774">
        <property type="entry name" value="SSF53774"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00144">
        <property type="entry name" value="ASN_GLN_ASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00917">
        <property type="entry name" value="ASN_GLN_ASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51732">
        <property type="entry name" value="ASN_GLN_ASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Asparaginase" evidence="8">
        <location>
            <begin position="7"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="domain" description="Asparaginase_C" evidence="9">
        <location>
            <begin position="217"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="4">
        <location>
            <begin position="96"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="active site" evidence="6">
        <location>
            <position position="16"/>
        </location>
    </feature>
    <feature type="active site" description="Acyl-ester intermediate" evidence="5">
        <location>
            <position position="16"/>
        </location>
    </feature>
    <feature type="active site" description="O-isoaspartyl threonine intermediate" evidence="3">
        <location>
            <position position="16"/>
        </location>
    </feature>
    <feature type="active site" evidence="7">
        <location>
            <position position="96"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="63"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010518"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR001220-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR001220-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR604550-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10099"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10100"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00710"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF17763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ABF08859.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="333" mass="34172" checksum="15F7F9AEAAB2D713" modified="2006-05-30" version="1">MSVLLPRIVVLATGGTIAGSSANPASSAHYQAATVPVSQLVQAVPALADVARVEAEQVAQVDSKDMMFDLWRMLAARIGYWSAQDDVAGIVITHGTDTLEETAMFLHLTQACPLPVVMTAAMRPSTSLSADGPLNLLDAVRVAASPVAVGKGALVVLNQQIHAARDVVKAHTSAVDAFHSPVAGPLGFVQDTYVHFSRTPGPDQAPRLPVPAAWPLVEIVASYAQPGRVGIDAMVAAGVLGFVVAAAGNGSVHETLTAALVDAAACGVAVARSSRTGAGHVAIPAPPRPAGGVFVSAGDLNPYKARVLLLLALAGDPSLGRDAARLQALFERY</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LM08</accession>
    <name>Q1LM08_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Transcriptional regulator, lysR family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_1939</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the LysR transcriptional regulatory family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08818.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1939"/>
    <dbReference type="EnsemblBacteria" id="ABF08818">
        <property type="protein sequence ID" value="ABF08818"/>
        <property type="gene ID" value="Rmet_1939"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1939"/>
    <dbReference type="eggNOG" id="COG0583">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039613_6_4_4"/>
    <dbReference type="OMA" id="FVEIAEC"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005119">
        <property type="entry name" value="LysR_subst-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000847">
        <property type="entry name" value="Tscrpt_reg_HTH_LysR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00126">
        <property type="entry name" value="HTH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03466">
        <property type="entry name" value="LysR_substrate"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00039">
        <property type="entry name" value="HTHLYSR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50931">
        <property type="entry name" value="HTH_LYSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH lysR-type" evidence="2">
        <location>
            <begin position="17"/>
            <end position="74"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF08818.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="312" mass="33796" checksum="D9B3866065BE324F" modified="2006-05-30" version="1">MAAKVNEARGTLMLMTPSLKQLRYFVEIVDAGNFSLAAERLFIAQSALSRQIRDMEATLQVELLVRKPRHVEPTAAGQAFYESARRILSDLAGAAVQARQVQRGGADAIRLLHSSSVVLTPQRCGWLHRWQSDHPAARFEVAQASSEQQSVDIEEGRADLGLARDPVLRRYPNIQIDPMAGERLVVAVCADHPMSRRESLTLSALREMPFVSLPHPERGGLSYRVAQLCQASGFYPTAAGAISRKLSQLSLVAAGFGVAVVPESMQWFGPDGVRLVPLSDAGATTRVVLLSRRDAPEPVAALRTLALSAGAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="98" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LM14</accession>
    <name>Q1LM14_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">ABC-type transporter, permease component: PepT family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">dppB</name>
        <name evidence="7" type="ordered locus">Rmet_1933</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cell membrane</location>
            <topology evidence="5">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the binding-protein-dependent transport system permease family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08812.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516664.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1933"/>
    <dbReference type="EnsemblBacteria" id="ABF08812">
        <property type="protein sequence ID" value="ABF08812"/>
        <property type="gene ID" value="Rmet_1933"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1933"/>
    <dbReference type="eggNOG" id="COG0601">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036879_0_1_4"/>
    <dbReference type="OMA" id="FREMNGF"/>
    <dbReference type="OrthoDB" id="1479518at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045621">
        <property type="entry name" value="BPD_transp_1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19300">
        <property type="entry name" value="BPD_transp_1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="SSF161098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="7"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="100"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="143"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="181"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="241"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="283"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="6">
        <location>
            <begin position="94"/>
            <end position="306"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABF08812.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="316" mass="34447" checksum="6B69BCCDC4FB01C2" modified="2006-05-30" version="1">MTYLLRRIAYALPILLGVALLCFSLVHIAPGDPLVSVLPPDASEDLRRQLTALYGFDKPFLEQFLHWLLRALHGDLGTSIATNRPVMSEVSVAVVNSVRLAAVATLIGFTFGCLFGFVAGYLRDSVPDRVASFLSVLGVSIPHYWLGMVLVIAFSVLLGWLPATGAGPNGSGDWGWDWEHIQYMLLPAVTMSVIPMGIVARTIRALVAEILSNEFIAGLRARGLSDLAVFRHVVKNVTPTALSVMGLQLGYLLGGSILIETVFSWPGTGFLLNSAIFQRDFPLLQGTILVLAVFFVMLNLLVDALQTLFDPRIQRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LM85</accession>
    <name>Q1LM85_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">ABC-type transporter, periplasmic component: HAAT family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_1862</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08741.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516589.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1862"/>
    <dbReference type="EnsemblBacteria" id="ABF08741">
        <property type="protein sequence ID" value="ABF08741"/>
        <property type="gene ID" value="Rmet_1862"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1862"/>
    <dbReference type="eggNOG" id="COG0683">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027128_3_0_4"/>
    <dbReference type="OMA" id="AKHNERN"/>
    <dbReference type="OrthoDB" id="538961at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028081">
        <property type="entry name" value="Leu-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028082">
        <property type="entry name" value="Peripla_BP_I"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13458">
        <property type="entry name" value="Peripla_BP_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53822">
        <property type="entry name" value="SSF53822"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193666" evidence="1">
        <location>
            <begin position="29"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="domain" description="Peripla_BP_6" evidence="2">
        <location>
            <begin position="30"/>
            <end position="375"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF08741.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="416" mass="45373" checksum="F9DEC0BFB0E82AA6" modified="2006-05-30" version="1">MTKLRPLVTGVAAVVALGSALVSGVAAADTVKIAFIDPLSGLMAPVGQNQLKSWQYVADVANQKNWAGAHKFEVVGFDNKLSPQESLTILKQAVDQNIRYIVQGNGSAVGLALEDAVAKHNERNPGKEIVYLNYAAVDPDMTNSKCNYWHFRLDANSDMKMEALTTYLAKDPNVKKVYLINQNYSFGHQVARAAKEYLKRKRPDIQIVGEDLHPLAQVKDFAPYAAKIKASGADTVITGNWGSDLALLIKAGKDAGLPVNYYTYYAATTGVPTAMGASGAEHVKYVGYYNPNNPTFKGGDIVEGFKKKYNDDFYVMAAYSGVAFLAKAMKDTGSTDPVKVAKAMEGMKVESMNGTVEMRNTDHQAQQPLVVATWTKVNGKDIKFDQENTGYGWKTNAQLDQFVAAQPTSCQMKRPN</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="97" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LM89</accession>
    <name>Q1LM89_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Leucine/isoleucine/valine transporter subunit, ATP-binding component of ABC superfamily</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">livF7</name>
        <name evidence="9" type="ordered locus">Rmet_1858</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08737.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516585.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1858"/>
    <dbReference type="EnsemblBacteria" id="ABF08737">
        <property type="protein sequence ID" value="ABF08737"/>
        <property type="gene ID" value="Rmet_1858"/>
    </dbReference>
    <dbReference type="GeneID" id="60825649"/>
    <dbReference type="KEGG" id="rme:Rmet_1858"/>
    <dbReference type="eggNOG" id="COG0410">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_2_4"/>
    <dbReference type="OMA" id="YVMGHGQ"/>
    <dbReference type="OrthoDB" id="1475841at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 7 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="1" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-1278">Translocase</keyword>
    <feature type="domain" description="ABC transporter" evidence="8">
        <location>
            <begin position="6"/>
            <end position="237"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ABF08737.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="237" mass="26328" checksum="3BAB57E4E8306BE4" modified="2006-05-30" version="1">MSTRMLEVTGLHAYYGKSHILHGVDAHVDQGEIVALLGRNGVGRSTMAKAILGMVKAEGSVKFRGEEMLGRRTFEIAHAGIGYVPENRDIFPTLTVRQNLTLGEKRNPRQAKPRWSVADMYQMFPRLKERENTPAGVLSGGEQQMLTLCRTLMGDPDLVLIDEPTEGLAPMIVTLVGDFLKTLKERGISVLLIEQKLAIALDISQRVYVMGHGHIVFEGTPVELKANAQIRKEWLEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="70" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LMT1</accession>
    <name>Q1LMT1_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Fimbrial subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_1664</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08545.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516393.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1664"/>
    <dbReference type="EnsemblBacteria" id="ABF08545">
        <property type="protein sequence ID" value="ABF08545"/>
        <property type="gene ID" value="Rmet_1664"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1664"/>
    <dbReference type="eggNOG" id="COG3539">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045639_0_0_4"/>
    <dbReference type="OMA" id="TGACDTC"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009289">
        <property type="term" value="C:pilus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007155">
        <property type="term" value="P:cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1090">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000259">
        <property type="entry name" value="Adhesion_dom_fimbrial"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036937">
        <property type="entry name" value="Adhesion_dom_fimbrial_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008966">
        <property type="entry name" value="Adhesion_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011228">
        <property type="entry name" value="UCP029766"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00419">
        <property type="entry name" value="Fimbrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF029766">
        <property type="entry name" value="UCP029766"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49401">
        <property type="entry name" value="SSF49401"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fimbrial" evidence="1">
        <location>
            <begin position="280"/>
            <end position="475"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00419"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF08545.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="475" mass="49007" checksum="E23CF0FE2E8FAE6B" modified="2006-05-30" version="1">MIRRLQIFTIRFGEGNLIPSLAALLFIVGIAGPMLALAQSTSKGCWAITGTTTNPNSYLYTDPGTVARSWIGAADTAGQGAYPMTISVNIPALVPDGTLLGSGVSSINNLGGSGVGSYSPEQVLFRCSPDADGTLYEYYATNGDAAYAGATDVSAQSGIPGTYLSYYTGVASRITNIATGDYLSRAWKARPLTGLDRDTQGWILVKAKNFSQYKLEMFQCTVCQSGGTNGTGSWAQSQPQGYVAFVGGATGTVIKGNLAVGADSASNYSGWYSTWPGAINAYQTVTVRRAATCAVTNATPLVRFPIISVRELNQGNTRQLPVTIQMQCQSSTPSGLSAFISGTAANQTALGVLVQPANAQSAVAAGLKTAGSGVTYLLSDGYGTDLSVATGVGVALSRPNGTPLNLLTNQYVTTGGTIDGWDSVLNDATANGPASGGVTSYTRSFNATLKAFAPGVTPVTAGRFNATAQVIVRVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="66" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LMU6</accession>
    <name>Q1LMU6_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_1647</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08530.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516375.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1647"/>
    <dbReference type="EnsemblBacteria" id="ABF08530">
        <property type="protein sequence ID" value="ABF08530"/>
        <property type="gene ID" value="Rmet_1647"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1647"/>
    <dbReference type="eggNOG" id="COG5343">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_075065_0_0_4"/>
    <dbReference type="OMA" id="KDYELWM"/>
    <dbReference type="OrthoDB" id="1740922at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018764">
        <property type="entry name" value="Anti-sigma_K_RskA"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10099">
        <property type="entry name" value="RskA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="101"/>
            <end position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF08530.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="242" mass="26108" checksum="64E508FC78C09783" modified="2006-05-30" version="1">MNLVRHPDLLDRMAAQYALGVLRGGARRRMEQMARQEPAVRVAIAHWQARLAGVAELQARAVPVEAVWCGIERRLGWKADPAEAQPTRTTGWRHVWQAASFWRGATAAMTVIALIAVIATIGLGPERRPSPSTVVALLQSQQSRAAMLVSWDPTGPALVVRRLDDLTLTNQQVLQLWALPVDGKPQSLGVIGRARQVRLPLATLPANVPTLAVSIEPLGGSTNPDGPSGPVVFHGALLKSPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LN08</accession>
    <name>Q1LN08_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Aconitate hydratase</fullName>
            <ecNumber evidence="3">4.2.1.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">acnA1</name>
        <name evidence="10" type="ordered locus">Rmet_1585</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>citrate = D-threo-isocitrate</text>
            <dbReference type="Rhea" id="RHEA:10336"/>
            <dbReference type="ChEBI" id="CHEBI:15562"/>
            <dbReference type="ChEBI" id="CHEBI:16947"/>
            <dbReference type="EC" id="4.2.1.3"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the aconitase/IPM isomerase family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.3" evidence="3"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08468.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516320.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1585"/>
    <dbReference type="EnsemblBacteria" id="ABF08468">
        <property type="protein sequence ID" value="ABF08468"/>
        <property type="gene ID" value="Rmet_1585"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1585"/>
    <dbReference type="eggNOG" id="COG1048">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013476_2_1_4"/>
    <dbReference type="OMA" id="GRASYMR"/>
    <dbReference type="OrthoDB" id="363064at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003994">
        <property type="term" value="F:aconitate hydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047780">
        <property type="term" value="F:citrate dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019679">
        <property type="term" value="P:propionate metabolic process, methylcitrate cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.19.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.499.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012708">
        <property type="entry name" value="2Me_IsoCit_deHydtase_FeS-dep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015931">
        <property type="entry name" value="Acnase/IPM_dHydase_lsu_aba_1/3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001030">
        <property type="entry name" value="Acoase/IPM_deHydtase_lsu_aba"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015928">
        <property type="entry name" value="Aconitase/3IPM_dehydase_swvl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006249">
        <property type="entry name" value="Aconitase/IRP2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036008">
        <property type="entry name" value="Aconitase_4Fe-4S_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000573">
        <property type="entry name" value="AconitaseA/IPMdHydase_ssu_swvl"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11670">
        <property type="entry name" value="PTHR11670"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11670:SF43">
        <property type="entry name" value="PTHR11670:SF43"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00330">
        <property type="entry name" value="Aconitase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00694">
        <property type="entry name" value="Aconitase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00415">
        <property type="entry name" value="ACONITASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53732">
        <property type="entry name" value="SSF53732"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02333">
        <property type="entry name" value="2met_isocit_dHY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0408">Iron</keyword>
    <keyword evidence="6" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="10" id="KW-0456">Lyase</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aconitase" evidence="8">
        <location>
            <begin position="70"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="domain" description="Aconitase_C" evidence="9">
        <location>
            <begin position="657"/>
            <end position="787"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ABF08468.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="865" mass="94686" checksum="59D9713628E1A715" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-01-15" modified="2022-05-25" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LND7</accession>
    <name>SSRP_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">SsrA-binding protein</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Small protein B</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">smpB</name>
        <name type="ordered locus">Rmet_1456</name>
    </gene>
    <organism>
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Required for rescue of stalled ribosomes mediated by trans-translation. Binds to transfer-messenger RNA (tmRNA), required for stable association of tmRNA with ribosomes. tmRNA and SmpB together mimic tRNA shape, replacing the anticodon stem-loop with SmpB. tmRNA is encoded by the ssrA gene; the 2 termini fold to resemble tRNA(Ala) and it encodes a 'tag peptide', a short internal open reading frame. During trans-translation Ala-aminoacylated tmRNA acts like a tRNA, entering the A-site of stalled ribosomes, displacing the stalled mRNA. The ribosome then switches to translate the ORF on the tmRNA; the nascent peptide is terminated with the 'tag peptide' encoded by the tmRNA and targeted for degradation. The ribosome is freed to recommence translation, which seems to be the essential function of trans-translation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
        <text evidence="1">The tmRNA-SmpB complex associates with stalled 70S ribosomes.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the SmpB family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08339.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008652065.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q1LND7"/>
    <dbReference type="SMR" id="Q1LND7"/>
    <dbReference type="STRING" id="266264.Rmet_1456"/>
    <dbReference type="EnsemblBacteria" id="ABF08339">
        <property type="protein sequence ID" value="ABF08339"/>
        <property type="gene ID" value="Rmet_1456"/>
    </dbReference>
    <dbReference type="GeneID" id="60822213"/>
    <dbReference type="KEGG" id="rme:Rmet_1456"/>
    <dbReference type="eggNOG" id="COG0691">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_108953_3_0_4"/>
    <dbReference type="OMA" id="WTNHSAR"/>
    <dbReference type="OrthoDB" id="1720952at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070929">
        <property type="term" value="P:trans-translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd09294">
        <property type="entry name" value="SmpB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.280.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00023">
        <property type="entry name" value="SmpB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023620">
        <property type="entry name" value="SmpB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000037">
        <property type="entry name" value="SsrA-bd_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020081">
        <property type="entry name" value="SsrA-bd_prot_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30308">
        <property type="entry name" value="PTHR30308"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01668">
        <property type="entry name" value="SmpB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF74982">
        <property type="entry name" value="SSF74982"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00086">
        <property type="entry name" value="smpB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01317">
        <property type="entry name" value="SSRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <feature type="chain" id="PRO_1000002122" description="SsrA-binding protein">
        <location>
            <begin position="1"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="124"/>
            <end position="150"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="150" mass="17301" checksum="8DA792CC1F482807" modified="2006-05-30" version="1">MTIADNKKAFFDYFIEERYEAGIVLEGWEVKAIRAGRVQIKEGYVVVRDAEMFLIGAHISPLQSASTHINPDPVRTRKLLLKADEIKKLIGKVEQRGYTLVPLNLHYTRGRVKCEIGLAKGKKQFDKRETEKNRDWDREKARIMKGGVKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LNM1</accession>
    <name>Q1LNM1_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Alkanesulfonate transporter subunit membrane component of ABC superfamily</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">ssuC</name>
        <name evidence="6" type="ordered locus">Rmet_1372</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cell membrane</location>
            <topology evidence="4">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the binding-protein-dependent transport system permease family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08255.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008649165.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1372"/>
    <dbReference type="EnsemblBacteria" id="ABF08255">
        <property type="protein sequence ID" value="ABF08255"/>
        <property type="gene ID" value="Rmet_1372"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1372"/>
    <dbReference type="eggNOG" id="COG0600">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046113_1_2_4"/>
    <dbReference type="OMA" id="LRWHPGY"/>
    <dbReference type="OrthoDB" id="1694312at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="SSF161098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2 4" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="21"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="78"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="112"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="137"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="177"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="227"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="5">
        <location>
            <begin position="71"/>
            <end position="251"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABF08255.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="268" mass="29384" checksum="DA92B787AC9FB136" modified="2006-05-30" version="1">MSAPHSIAPRRIDWRQAARAIAPWIVPILLIVVWQVASQAGWLSNRVLPAPLAVVEAAWTLAASGELWKHVWISTWRALVGLAIGGGLGLVLGLLTGTFRTAATLLDSTLQMVRNIPPLALIPLVILWFGIDETAKLFLVALGVFFPVYLNTYHGIRAVDPALVEMARSYGLSGWRLYREVILPGALPGILVGVRFSLGLMWVILIVAETISAQSGIGYMTMNAREFLQTDVVLVGILLYALLGKLADVLSRALERHWLRWHPGYLSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LNQ2</accession>
    <name>Q1LNQ2_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">P-type conjugative transfer ATPase TrbB (CMGI-2)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">trbB</name>
        <name evidence="3" type="ordered locus">Rmet_1341</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the GSP E family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08224.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1341"/>
    <dbReference type="EnsemblBacteria" id="ABF08224">
        <property type="protein sequence ID" value="ABF08224"/>
        <property type="gene ID" value="Rmet_1341"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1341"/>
    <dbReference type="eggNOG" id="COG4962">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005379_3_0_4"/>
    <dbReference type="OMA" id="FNYEDAL"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014149">
        <property type="entry name" value="Conjug-transfer_TrbB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001482">
        <property type="entry name" value="T2SS/T4SS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00437">
        <property type="entry name" value="T2SSE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02782">
        <property type="entry name" value="TrbB_P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="T2SP_E" evidence="2">
        <location>
            <begin position="34"/>
            <end position="295"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006611"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF08224.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="354" mass="37492" checksum="09A84BBC707CB7CB" modified="2006-05-30" version="1">MSARISIEGQSMSATSLDRRIQMLRTAMGPLIAAALEDPDVVEVMLNPDRTLWVDRLSSGRAPMGVELPEADGERIIRLVAAHVGAEVHRGQPLLSAELPETGERFEGILPPAAPGPAFALRKRAIGVIPLERYVVEGMMTAAQAGFLVRAVRERQNVLIAGATSSGKTTLANALLAEIAATGDRVLVLEDTVELQCAARDHVPLRTRAGVVSMTELVRSSMRLRPDRVVVGEVRGAEALDLIKVWGTGHPGGIATIHAGSALGALLRLEQLILEVAVNPPRALIAEAVNVVIHIAGRGRKRRIESIARVVGFDGVGYQLADALETSFPELLPIPDAASAAATSPSPDQPGELP</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="50" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LNZ7</accession>
    <name>Q1LNZ7_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_1243</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08129.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516015.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF08129">
        <property type="protein sequence ID" value="ABF08129"/>
        <property type="gene ID" value="Rmet_1243"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1243"/>
    <dbReference type="eggNOG" id="ENOG502ZC17">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_126999_0_0_4"/>
    <dbReference type="OMA" id="WGLCPEH"/>
    <dbReference type="OrthoDB" id="1609626at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF08129.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="130" mass="14201" checksum="FA0569FFB97FB376" modified="2006-05-30" version="1">MHDKSHVSLEQHVCLVCGTAFDTGAILLDKRLRASMERHTATGWGLCPEHQKLSDDGFVALVECDPQRSGSQAGGRMKPEQAYRTGRLAHLRRTLFAQVFNVPIAAEQACVFVEPGVIDQLQSMTAPAAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="115" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LP17</accession>
    <name>Q1LP17_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Shikimate kinase</fullName>
            <shortName evidence="2">SK</shortName>
            <ecNumber evidence="2">2.7.1.71</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">bzdR</name>
        <name evidence="2" type="synonym">aroK</name>
        <name evidence="5" type="ordered locus">Rmet_1223</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Catalyzes the specific phosphorylation of the 3-hydroxyl group of shikimic acid using ATP as a cosubstrate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>ATP + shikimate = 3-phosphoshikimate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13121"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:36208"/>
            <dbReference type="ChEBI" id="CHEBI:145989"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.71"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="2">Binds 1 Mg(2+) ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 5/7.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the shikimate kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.71" evidence="2"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08109.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515998.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1223"/>
    <dbReference type="EnsemblBacteria" id="ABF08109">
        <property type="protein sequence ID" value="ABF08109"/>
        <property type="gene ID" value="Rmet_1223"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1223"/>
    <dbReference type="eggNOG" id="COG0703">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG1396">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_908130_0_0_4"/>
    <dbReference type="OMA" id="AYRRYEM"/>
    <dbReference type="OrthoDB" id="1542870at2"/>
    <dbReference type="UniPathway" id="UPA00053">
        <property type="reaction ID" value="UER00088"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004765">
        <property type="term" value="F:shikimate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009073">
        <property type="term" value="P:aromatic amino acid family biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008652">
        <property type="term" value="P:cellular amino acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009423">
        <property type="term" value="P:chorismate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00093">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00464">
        <property type="entry name" value="SK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00109">
        <property type="entry name" value="Shikimate_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001387">
        <property type="entry name" value="Cro/C1-type_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010982">
        <property type="entry name" value="Lambda_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031322">
        <property type="entry name" value="Shikimate/glucono_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000623">
        <property type="entry name" value="Shikimate_kinase/TSH1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01381">
        <property type="entry name" value="HTH_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01202">
        <property type="entry name" value="SKI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00530">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47413">
        <property type="entry name" value="SSF47413"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50943">
        <property type="entry name" value="HTH_CROC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="1 2" id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 5" id="KW-0418">Kinase</keyword>
    <keyword evidence="2" id="KW-0460">Magnesium</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="HTH cro/C1-type" evidence="4">
        <location>
            <begin position="34"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="155"/>
            <end position="160"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="159"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="201"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="224"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="263"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="282"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="298"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABF08109.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="316" mass="34599" checksum="D8109B6DC506328A" modified="2006-05-30" version="1">MRRDPDPLMTAEGRPDNRATGERDPYLTHLGERIRSLRAARGMSRKDLARGADVSERYLANLETGTGNASVLLLRQVANALDVPLPVVLAEVDGHADAGGAGNPRATEFSQLIQWLAQLPASDLARVREAARHALSPTESRDARHRRIALIGLRGAGKSTLGRALAAAEDMPFVELNAAIEREAGASLSEIHSLYGQAAYRRYEMRALERVLRENDTMVLATPGSLVSEPATFNLLLSRCFTIWVRTSPEEHMARVVAQGDMRPMEGNREAMTDLRRILQARTPLYSRADATIDTSGQDAATSLHALQAQILHTRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="59" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LP34</accession>
    <name>Q1LP34_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative hydroxyacid oxidoreductase (Fe-S centre)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">ykgE</name>
        <name evidence="2" type="ordered locus">Rmet_1206</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08092.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515983.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1206"/>
    <dbReference type="EnsemblBacteria" id="ABF08092">
        <property type="protein sequence ID" value="ABF08092"/>
        <property type="gene ID" value="Rmet_1206"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1206"/>
    <dbReference type="eggNOG" id="COG0247">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023081_1_0_4"/>
    <dbReference type="OMA" id="TEMPACE"/>
    <dbReference type="OrthoDB" id="647535at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004017">
        <property type="entry name" value="Cys_rich_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02754">
        <property type="entry name" value="CCG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CCG" evidence="1">
        <location>
            <begin position="4"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="domain" description="CCG" evidence="1">
        <location>
            <begin position="132"/>
            <end position="216"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02754"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF08092.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="241" mass="26644" checksum="1B7470923DD53081" modified="2006-05-30" version="1">MRIGLFATCLVDLMRPEIGFSVLKLLESAGYEVMVPEAQTCCGQPAYNSGERAVSRDLAEKFLREFEMFDYVVVPSGSCGGMIRQHYQDLLRDDPELHGRYERLRERVFELTDFLVNVAKIDRFDSTFAGRVTYHDSCSGLRELGVKQQPRELLGKLPGVTLTEMKDCEACCGFGGTFSVKYGNISTAIVDEKCANIQSSGADAVVLGDLGCILNIEGRLRRTGDTRTRVLHIAQVLAGDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="113" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LP75</accession>
    <name>Q1LP75_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Phenylalanine--tRNA ligase beta subunit</fullName>
            <ecNumber evidence="14">6.1.1.20</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Phenylalanyl-tRNA synthetase beta subunit</fullName>
            <shortName evidence="14">PheRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14 19" type="primary">pheT</name>
        <name evidence="19" type="ordered locus">Rmet_1165</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 14">
            <text>ATP + L-phenylalanine + tRNA(Phe) = AMP + diphosphate + H(+) + L-phenylalanyl-tRNA(Phe)</text>
            <dbReference type="Rhea" id="RHEA:19413"/>
            <dbReference type="Rhea" id="RHEA-COMP:9668"/>
            <dbReference type="Rhea" id="RHEA-COMP:9699"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58095"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78531"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.20"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="14">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="14">Binds 2 magnesium ions per tetramer.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4 14">Tetramer of two alpha and two beta subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 14">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 14">Belongs to the phenylalanyl-tRNA synthetase beta subunit family. Type 1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.20" evidence="14"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08051.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515947.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1165"/>
    <dbReference type="EnsemblBacteria" id="ABF08051">
        <property type="protein sequence ID" value="ABF08051"/>
        <property type="gene ID" value="Rmet_1165"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1165"/>
    <dbReference type="eggNOG" id="COG0072">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG0073">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016891_0_0_4"/>
    <dbReference type="OMA" id="ISYNWLK"/>
    <dbReference type="OrthoDB" id="53568at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004826">
        <property type="term" value="F:phenylalanine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006432">
        <property type="term" value="P:phenylalanyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00769">
        <property type="entry name" value="PheRS_beta_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02796">
        <property type="entry name" value="tRNA_bind_bactPheRS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.380">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00283">
        <property type="entry name" value="Phe_tRNA_synth_beta1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005146">
        <property type="entry name" value="B3/B4_tRNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009061">
        <property type="entry name" value="DNA-bd_dom_put_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005121">
        <property type="entry name" value="Fdx_antiC-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036690">
        <property type="entry name" value="Fdx_antiC-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045060">
        <property type="entry name" value="Phe-tRNA-ligase_IIc_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004532">
        <property type="entry name" value="Phe-tRNA-ligase_IIc_bsu_bact"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020825">
        <property type="entry name" value="Phe-tRNA_synthase-like_B3/B4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041616">
        <property type="entry name" value="PheRS_beta_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002547">
        <property type="entry name" value="tRNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033714">
        <property type="entry name" value="tRNA_bind_bactPheRS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005147">
        <property type="entry name" value="tRNA_synthase_B5-dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10947">
        <property type="entry name" value="PTHR10947"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03483">
        <property type="entry name" value="B3_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03484">
        <property type="entry name" value="B5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03147">
        <property type="entry name" value="FDX-ACB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01588">
        <property type="entry name" value="tRNA_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17759">
        <property type="entry name" value="tRNA_synthFbeta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00873">
        <property type="entry name" value="B3_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00874">
        <property type="entry name" value="B5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00896">
        <property type="entry name" value="FDX-ACB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46955">
        <property type="entry name" value="SSF46955"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54991">
        <property type="entry name" value="SSF54991"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="SSF55681"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00472">
        <property type="entry name" value="pheT_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51483">
        <property type="entry name" value="B5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51447">
        <property type="entry name" value="FDX_ACB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50886">
        <property type="entry name" value="TRBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13 14 19" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="9 14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="14" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 14 19" id="KW-0436">Ligase</keyword>
    <keyword evidence="10 14" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7 14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12 14" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11 15" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="5 15" id="KW-0820">tRNA-binding</keyword>
    <feature type="domain" description="TRNA-binding" evidence="16">
        <location>
            <begin position="40"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="domain" description="B5" evidence="18">
        <location>
            <begin position="406"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="domain" description="FDX-ACB" evidence="17">
        <location>
            <begin position="712"/>
            <end position="814"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="463"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="469"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="472"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="473"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000395"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008653"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="ABF08051.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="815" mass="89313" checksum="A0A3E4EF2FB06FEA" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LPH4</accession>
    <name>Q1LPH4_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="16">Zn-dependent membrane-bound protease, M50 family</fullName>
            <ecNumber evidence="16">3.4.24.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16" type="ordered locus">Rmet_1066</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the peptidase M50B family.</text>
    </comment>
    <dbReference type="EC" id="3.4.24.-" evidence="16"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07952.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515854.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1066"/>
    <dbReference type="EnsemblBacteria" id="ABF07952">
        <property type="protein sequence ID" value="ABF07952"/>
        <property type="gene ID" value="Rmet_1066"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1066"/>
    <dbReference type="eggNOG" id="COG1994">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_086979_0_0_4"/>
    <dbReference type="OMA" id="HEIMHGL"/>
    <dbReference type="OrthoDB" id="1401629at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06158">
        <property type="entry name" value="S2P-M50_like_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008915">
        <property type="entry name" value="Peptidase_M50"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044537">
        <property type="entry name" value="S2P-M50-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02163">
        <property type="entry name" value="Peptidase_M50"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9 16" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="13 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="6 16" id="KW-0645">Protease</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 14" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="10" id="KW-0862">Zinc</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="56"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="97"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="134"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="172"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase_M50" evidence="15">
        <location>
            <begin position="132"/>
            <end position="188"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF02163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="ABF07952.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="221" mass="24115" checksum="C28E467D821CC189" modified="2006-05-30" version="1">MDSSLIQTFAVYALPVLFAITLHEAAHGYVAKHFGDNTAYLLGRVSLNPVRHIDPIGTVAVPLLLYFLTAGQFVFGFAKPVPVAFDRLRNPRWHGMWVALAGPASNIVQAFAWALVAIGLALAHIQEPFWSEMARAGVLVNTVVAAFNLFPIPPLDGGRVLTALLPQRLAGVMARIEPYGIFVVLALVAAGVITKIWMRPVMNGLLSIVELMLRPVMWLLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="92" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LPI2</accession>
    <name>Q1LPI2_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Heat shock protein (Hsp33-like)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">hslO</name>
        <name evidence="6" type="ordered locus">Rmet_1058</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07944.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515847.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1058"/>
    <dbReference type="EnsemblBacteria" id="ABF07944">
        <property type="protein sequence ID" value="ABF07944"/>
        <property type="gene ID" value="Rmet_1058"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1058"/>
    <dbReference type="eggNOG" id="COG1281">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_054493_0_0_4"/>
    <dbReference type="OMA" id="DMQCECC"/>
    <dbReference type="OrthoDB" id="1406579at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00498">
        <property type="entry name" value="Hsp33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.480">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.55.30.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1280.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000397">
        <property type="entry name" value="Heat_shock_Hsp33"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016154">
        <property type="entry name" value="Heat_shock_Hsp33_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016153">
        <property type="entry name" value="Heat_shock_Hsp33_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023212">
        <property type="entry name" value="Hsp33_helix_hairpin_bin_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30111">
        <property type="entry name" value="PTHR30111"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01430">
        <property type="entry name" value="HSP33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005261">
        <property type="entry name" value="Heat_shock_Hsp33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF118352">
        <property type="entry name" value="SSF118352"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64397">
        <property type="entry name" value="SSF64397"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0143">Chaperone</keyword>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0346">Stress response</keyword>
    <keyword evidence="2" id="KW-0862">Zinc</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABF07944.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="334" mass="36347" checksum="24A67E6089F68609" modified="2006-05-30" version="1">MTATSNPDTLQKFLFDAAPVRGELVRMEATWQEVLNRHAYPAPVRRLLGEMMAAAALLSANLKFNGALVMQLHGDGPVRMLVVECLSDLSMRATAKIAEDATITDDATLTTLVNAHGHGRFAITLDPQDKLPGQQPYQGIVPLSDENGPLDSMAAVLEHYMQASEQLDTRLWLASNEQVAAGMLLQKLPSYGGTMEAQVGETGSPLSGDVQAQDLDTWDRACQLGATLKQDELLAESPETLLRRLFWEELQNAGLRVFEPMTPHFQCSCSRAKVAGMLQSLGQAEIESIIAERGDVEIHCDFCGQRYVFDPVDAAQLFTLPVATGAGEGAQQQH</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="103" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LPI7</accession>
    <name>Q1LPI7_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">2-dehydro-3-deoxyphosphooctonate aldolase</fullName>
            <ecNumber evidence="6">2.5.1.55</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">3-deoxy-D-manno-octulosonic acid 8-phosphate synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">KDO-8-phosphate synthase</fullName>
            <shortName evidence="6">KDO 8-P synthase</shortName>
            <shortName evidence="6">KDOPS</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">Phospho-2-dehydro-3-deoxyoctonate aldolase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6 8" type="primary">kdsA</name>
        <name evidence="8" type="ordered locus">Rmet_1053</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 6">
            <text>D-arabinose 5-phosphate + H2O + phosphoenolpyruvate = 3-deoxy-alpha-D-manno-2-octulosonate-8-phosphate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:14053"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57693"/>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
            <dbReference type="ChEBI" id="CHEBI:85985"/>
            <dbReference type="EC" id="2.5.1.55"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3 6">Bacterial outer membrane biogenesis; lipopolysaccharide biosynthesis.</text>
    </comment>
    <comment type="pathway">
        <text evidence="4 6">Carbohydrate biosynthesis; 3-deoxy-D-manno-octulosonate biosynthesis; 3-deoxy-D-manno-octulosonate from D-ribulose 5-phosphate: step 2/3.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5 6">Belongs to the KdsA family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.55" evidence="6"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07939.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515845.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1053"/>
    <dbReference type="EnsemblBacteria" id="ABF07939">
        <property type="protein sequence ID" value="ABF07939"/>
        <property type="gene ID" value="Rmet_1053"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1053"/>
    <dbReference type="eggNOG" id="COG2877">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036666_0_0_4"/>
    <dbReference type="OMA" id="PHISQGV"/>
    <dbReference type="OrthoDB" id="687380at2"/>
    <dbReference type="UniPathway" id="UPA00030"/>
    <dbReference type="UniPathway" id="UPA00357">
        <property type="reaction ID" value="UER00474"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008676">
        <property type="term" value="F:3-deoxy-8-phosphooctulonate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019294">
        <property type="term" value="P:keto-3-deoxy-D-manno-octulosonic acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00056">
        <property type="entry name" value="KDO8P_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006218">
        <property type="entry name" value="DAHP1/KDSA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006269">
        <property type="entry name" value="KDO8P_synthase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21057">
        <property type="entry name" value="PTHR21057"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00793">
        <property type="entry name" value="DAHP_synth_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01362">
        <property type="entry name" value="KDO8P_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0448">Lipopolysaccharide biosynthesis</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="DAHP_synth_1" evidence="7">
        <location>
            <begin position="8"/>
            <end position="273"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004756"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00010499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ABF07939.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="289" mass="31004" checksum="70585766C05A1979" modified="2006-05-30" version="1">MKLCGFDVGLDKPFFLIAGPCVIESEQMALDTAGELKAITGELGIPFIYKSSFDKANRSSGKSFRGLGMEKGLEILATVKRQIGVPVLTDIHEIDEIKPVAAVVDVLQTPAFLCRQTDFIRACAQSGKPVNIKKGQFLAPHDMKNVIDKARDAAREAGLPDDVFMACERGVSFGYNNLVSDMRSLAIMRETGAPVVFDATHSVQLPGGQGTSSGGQREFVPVLSRAAVATGVAGLFMETHPDPSKAMSDGPNAVPLSRMKELLTVLRDLDGMVKRAGFLEDNFGWPACA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LPQ4</accession>
    <name>Q1LPQ4_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative ATPase, AAA family</fullName>
            <ecNumber evidence="2">3.6.1.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_0986</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EC" id="3.6.1.-" evidence="2"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07872.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008641739.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0986"/>
    <dbReference type="EnsemblBacteria" id="ABF07872">
        <property type="protein sequence ID" value="ABF07872"/>
        <property type="gene ID" value="Rmet_0986"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0986"/>
    <dbReference type="eggNOG" id="COG0714">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_051820_2_1_4"/>
    <dbReference type="OMA" id="YVYETRE"/>
    <dbReference type="OrthoDB" id="489714at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA" evidence="1">
        <location>
            <begin position="37"/>
            <end position="195"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF07872.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="292" mass="33288" checksum="8E5940D4473ABD03" modified="2006-05-30" version="1">MSTTANANPSQQVKFEGSAQYVATDDLKLAVNAARTLQRPLLIKGEPGTGKTMLAEEVAAALGMPLLQWHIKSTTKAQQGLYEYDAVSRLRDSQLGDNRVHDIANYIVKGVLWQAFEADEPVVLLIDEIDKADIEFPNDLLRELDRMEFYVYETRELIQAKHRPLVIITSNNEKELPDAFLRRCFFHYIKFPDAETMQQIVDVHYPGIKRELVAAAMESFYEMRNLPGLKKKPSTSELIDWLKLLMAEDIPAEALRSPDRKAAIPPLHGALLKNEQDVHLFERLVFMNRANR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="97" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LPU2</accession>
    <name>Q1LPU2_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Ubiquinol oxidase subunit 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">cyoA</name>
        <name evidence="15" type="ordered locus">Rmet_0948</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="11">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Periplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the cytochrome c oxidase subunit 2 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07834.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0948"/>
    <dbReference type="EnsemblBacteria" id="ABF07834">
        <property type="protein sequence ID" value="ABF07834"/>
        <property type="gene ID" value="Rmet_0948"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0948"/>
    <dbReference type="eggNOG" id="COG1622">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036876_6_1_4"/>
    <dbReference type="OMA" id="TSLDWKW"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042597">
        <property type="term" value="C:periplasmic space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070469">
        <property type="term" value="C:respirasome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009486">
        <property type="term" value="F:cytochrome bo3 ubiquinol oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004129">
        <property type="term" value="F:cytochrome-c oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016682">
        <property type="term" value="F:oxidoreductase activity, acting on diphenols and related substances as donors, oxygen as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04212">
        <property type="entry name" value="CuRO_UO_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.420">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045187">
        <property type="entry name" value="CcO_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002429">
        <property type="entry name" value="CcO_II-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010514">
        <property type="entry name" value="COX_ARM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008972">
        <property type="entry name" value="Cupredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034227">
        <property type="entry name" value="CuRO_UO_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011759">
        <property type="entry name" value="Cyt_c_oxidase_su2_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036257">
        <property type="entry name" value="Cyt_c_oxidase_su2_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006333">
        <property type="entry name" value="Cyt_o_ubiquinol_oxidase_su2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22888">
        <property type="entry name" value="PTHR22888"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00116">
        <property type="entry name" value="COX2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06481">
        <property type="entry name" value="COX_ARM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000292">
        <property type="entry name" value="Ubi_od_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49503">
        <property type="entry name" value="SSF49503"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81464">
        <property type="entry name" value="SSF81464"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01433">
        <property type="entry name" value="CyoA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50857">
        <property type="entry name" value="COX2_CUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50999">
        <property type="entry name" value="COX2_TM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 11" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8 11" id="KW-0249">Electron transport</keyword>
    <keyword evidence="10 11 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="11 15" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 11" id="KW-0679">Respiratory chain</keyword>
    <keyword evidence="7 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4 11" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="39"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="87"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="domain" description="COX2_TM" evidence="14">
        <location>
            <begin position="20"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="domain" description="COX2_CUA" evidence="13">
        <location>
            <begin position="123"/>
            <end position="235"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007866"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRNR" id="PIRNR000292"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="ABF07834.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="322" mass="35459" checksum="0173569E96DAF1E3" modified="2006-05-30" version="1">MKKPKMPPLMRYLPCLGLLLLAGCDFALMNPKGQVGVDIKGIILIATWLMLLVVVPVIILTLVFAWKYRASNTSAEYDPNWSHSTRIEVVVWLIPCLIIIALGIITWKSSHDLDPYKPLESNVKPVTVEAVAMNWKWLFIYPELKIATVNQLALPVNTPVNFKITSDSIMNSFFIPQLGSQVYAMAGMETKLHLIANEAGTFDGMSANYSGGGFSGMKFKAIAMSNFEFDKWVEKVRASEKQLASEDYKVLAKPSEAEPVTYFGKVEDGLYTGILHQYMGGDGHAMAREGFDGGPVCTSRNTLGEERVSMATPAKPALGAQI</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-05-05" modified="2022-05-25" version="106" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LPW0</accession>
    <name>NUOD_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">NADH-quinone oxidoreductase subunit D</fullName>
            <ecNumber evidence="1">7.1.1.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">NADH dehydrogenase I subunit D</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">NDH-1 subunit D</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">nuoD</name>
        <name type="ordered locus">Rmet_0930</name>
    </gene>
    <organism>
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a quinone + 5 H(+)(in) + NADH = a quinol + 4 H(+)(out) + NAD(+)</text>
            <dbReference type="Rhea" id="RHEA:57888"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:24646"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:132124"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">NDH-1 is composed of 14 different subunits. Subunits NuoB, C, D, E, F, and G constitute the peripheral sector of the complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell inner membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the complex I 49 kDa subunit family.</text>
    </comment>
    <dbReference type="EC" id="7.1.1.-" evidence="1"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07816.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515748.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q1LPW0"/>
    <dbReference type="SMR" id="Q1LPW0"/>
    <dbReference type="STRING" id="266264.Rmet_0930"/>
    <dbReference type="EnsemblBacteria" id="ABF07816">
        <property type="protein sequence ID" value="ABF07816"/>
        <property type="gene ID" value="Rmet_0930"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0930"/>
    <dbReference type="eggNOG" id="COG0649">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015134_1_1_4"/>
    <dbReference type="OMA" id="IMGTSME"/>
    <dbReference type="OrthoDB" id="473681at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050136">
        <property type="term" value="F:NADH dehydrogenase (quinone) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048038">
        <property type="term" value="F:quinone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.645.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01358">
        <property type="entry name" value="NDH1_NuoD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001135">
        <property type="entry name" value="NADH_Q_OxRdtase_suD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014029">
        <property type="entry name" value="NADH_UbQ_OxRdtase_49kDa_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022885">
        <property type="entry name" value="NDH1_su_D/H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029014">
        <property type="entry name" value="NiFe-Hase_large"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11993">
        <property type="entry name" value="PTHR11993"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00346">
        <property type="entry name" value="Complex1_49kDa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56762">
        <property type="entry name" value="SSF56762"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01962">
        <property type="entry name" value="NuoD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00535">
        <property type="entry name" value="COMPLEX1_49K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0997">Cell inner membrane</keyword>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-0520">NAD</keyword>
    <keyword id="KW-0874">Quinone</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-1278">Translocase</keyword>
    <keyword id="KW-0813">Transport</keyword>
    <keyword id="KW-0830">Ubiquinone</keyword>
    <feature type="chain" id="PRO_0000371919" description="NADH-quinone oxidoreductase subunit D">
        <location>
            <begin position="1"/>
            <end position="417"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01358"/>
        </source>
    </evidence>
    <sequence length="417" mass="47833" checksum="37C1465E1EEC92D0" modified="2006-05-30" version="1">MADIKNYTLNFGPQHPAAHGVLRLVLELDGEVIQRADPHIGLLHRATEKLAEQKTWIQSVPYMDRLDYVSMMVNEHAYVMAIERLLGLEVPIRAQYIRVMFDEITRLLNHLMWIGSHALDVGAMAVFLYAFREREDMFDMYEAVSGARMHAAYYRPGGVYRDLPDTMPQYRASKVHNEKAIKVMNEARSGSLLDFIEDFTNRFPTYVDEYETLLTDNRIWKQRLVDIGVVTPERALQMGFTGPMLRGSGIEWDLRKKQPYEVYDKMDFDIPVGTAGDCYSRYLVRVEEMRQSNRIIKQCIDWLRRNPGPVITENHKVAPPSRVDMKSNMEELIHHFKLFTEGIHVPAGEAYAAVEHPKGEFGIYAISDGANKPYRLKIRAPGFVHLAALDEMARGHMIADAVTIIGTQDIVFGEIDR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-09-02" modified="2022-08-03" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LQ59</accession>
    <name>SYA_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Alanine--tRNA ligase</fullName>
            <ecNumber evidence="1">6.1.1.7</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Alanyl-tRNA synthetase</fullName>
            <shortName evidence="1">AlaRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">alaS</name>
        <name type="ordered locus">Rmet_0831</name>
    </gene>
    <organism>
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the attachment of alanine to tRNA(Ala) in a two-step reaction: alanine is first activated by ATP to form Ala-AMP and then transferred to the acceptor end of tRNA(Ala). Also edits incorrectly charged Ser-tRNA(Ala) and Gly-tRNA(Ala) via its editing domain.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + L-alanine + tRNA(Ala) = AMP + diphosphate + L-alanyl-tRNA(Ala)</text>
            <dbReference type="Rhea" id="RHEA:12540"/>
            <dbReference type="Rhea" id="RHEA-COMP:9657"/>
            <dbReference type="Rhea" id="RHEA-COMP:9923"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57972"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78497"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.7"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="1">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="1">Consists of three domains; the N-terminal catalytic domain, the editing domain and the C-terminal C-Ala domain. The editing domain removes incorrectly charged amino acids, while the C-Ala domain, along with tRNA(Ala), serves as a bridge to cooperatively bring together the editing and aminoacylation centers thus stimulating deacylation of misacylated tRNAs.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-II aminoacyl-tRNA synthetase family.</text>
    </comment>
    <comment type="sequence caution" evidence="2">
        <conflict type="erroneous initiation">
            <sequence resource="EMBL-CDS" id="ABF07717" version="1"/>
        </conflict>
    </comment>
    <dbReference type="EC" id="6.1.1.7" evidence="1"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07717.1"/>
        <property type="status" value="ALT_INIT"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_029308285.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q1LQ59"/>
    <dbReference type="SMR" id="Q1LQ59"/>
    <dbReference type="STRING" id="266264.Rmet_0831"/>
    <dbReference type="PRIDE" id="Q1LQ59"/>
    <dbReference type="EnsemblBacteria" id="ABF07717">
        <property type="protein sequence ID" value="ABF07717"/>
        <property type="gene ID" value="Rmet_0831"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0831"/>
    <dbReference type="eggNOG" id="COG0013">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004485_1_1_4"/>
    <dbReference type="OrthoDB" id="91428at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004813">
        <property type="term" value="F:alanine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006419">
        <property type="term" value="P:alanyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00036_B">
        <property type="entry name" value="Ala_tRNA_synth_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002318">
        <property type="entry name" value="Ala-tRNA-lgiase_IIc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018162">
        <property type="entry name" value="Ala-tRNA-ligase_IIc_anticod-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018165">
        <property type="entry name" value="Ala-tRNA-synth_IIc_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018164">
        <property type="entry name" value="Ala-tRNA-synth_IIc_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023033">
        <property type="entry name" value="Ala_tRNA_ligase_euk/bac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003156">
        <property type="entry name" value="DHHA1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018163">
        <property type="entry name" value="Thr/Ala-tRNA-synth_IIc_edit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012947">
        <property type="entry name" value="tRNA_SAD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02272">
        <property type="entry name" value="DHHA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01411">
        <property type="entry name" value="tRNA-synt_2c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07973">
        <property type="entry name" value="tRNA_SAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00980">
        <property type="entry name" value="TRNASYNTHALA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00863">
        <property type="entry name" value="tRNA_SAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101353">
        <property type="entry name" value="SSF101353"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="SSF50447"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55186">
        <property type="entry name" value="SSF55186"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="SSF55681"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00344">
        <property type="entry name" value="alaS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50860">
        <property type="entry name" value="AA_TRNA_LIGASE_II_ALA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0820">tRNA-binding</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <feature type="chain" id="PRO_0000347748" description="Alanine--tRNA ligase">
        <location>
            <begin position="1"/>
            <end position="874"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="564"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="568"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="665"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="669"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="874" mass="94706" checksum="39B4BDE49BCAC1BF" modified="2008-09-02" version="2"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-10-14" modified="2022-05-25" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LQE1</accession>
    <name>RIMM_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Ribosome maturation factor RimM</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rimM</name>
        <name type="ordered locus">Rmet_0749</name>
    </gene>
    <organism>
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">An accessory protein needed during the final step in the assembly of 30S ribosomal subunit, possibly for assembly of the head region. Probably interacts with S19. Essential for efficient processing of 16S rRNA. May be needed both before and after RbfA during the maturation of 16S rRNA. It has affinity for free ribosomal 30S subunits but not for 70S ribosomes.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Binds ribosomal protein S19.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="1">The PRC barrel domain binds ribosomal protein S19.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the RimM family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07635.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515591.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q1LQE1"/>
    <dbReference type="SMR" id="Q1LQE1"/>
    <dbReference type="STRING" id="266264.Rmet_0749"/>
    <dbReference type="EnsemblBacteria" id="ABF07635">
        <property type="protein sequence ID" value="ABF07635"/>
        <property type="gene ID" value="Rmet_0749"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0749"/>
    <dbReference type="eggNOG" id="COG0806">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_077636_1_0_4"/>
    <dbReference type="OMA" id="DEFYHAD"/>
    <dbReference type="OrthoDB" id="1497415at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043022">
        <property type="term" value="F:ribosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042274">
        <property type="term" value="P:ribosomal small subunit biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00014">
        <property type="entry name" value="Ribosome_mat_RimM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011961">
        <property type="entry name" value="16S_RimM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027275">
        <property type="entry name" value="PRC-brl_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011033">
        <property type="entry name" value="PRC_barrel-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002676">
        <property type="entry name" value="RimM_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036976">
        <property type="entry name" value="RimM_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05239">
        <property type="entry name" value="PRC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01782">
        <property type="entry name" value="RimM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50346">
        <property type="entry name" value="SSF50346"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="SSF50447"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02273">
        <property type="entry name" value="16S_RimM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0143">Chaperone</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0690">Ribosome biogenesis</keyword>
    <feature type="chain" id="PRO_0000351789" description="Ribosome maturation factor RimM">
        <location>
            <begin position="1"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="domain" description="PRC barrel" evidence="1">
        <location>
            <begin position="143"/>
            <end position="220"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00014"/>
        </source>
    </evidence>
    <sequence length="220" mass="23635" checksum="8909AEEEE723C486" modified="2006-05-30" version="1">MTGNASGRKPLNLAATQGGASGRQKKLPAALLFTDTLPDDLVEVGYVGAAYGIRGWIKVQPHADDASALLHARRWWLLRAPPAGVVSAPAQSAEAVSIKISQSREHSGTVVAQPAGVSDRSVAEMLKGRRVWVRRADFPAAEEGEFYWVDLIGCAVVNEQGEALGEVTGLIDNGAHQILQVAYELPDGKAAERLIPFVDAFLRTVDTPARRIVVDWGLDY</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="97" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LQE9</accession>
    <name>Q1LQE9_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">DNA-binding protein transcriptional dual regulator, leucine-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">lrp</name>
        <name evidence="5" type="ordered locus">Rmet_0741</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07627.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008643687.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0741"/>
    <dbReference type="EnsemblBacteria" id="ABF07627">
        <property type="protein sequence ID" value="ABF07627"/>
        <property type="gene ID" value="Rmet_0741"/>
    </dbReference>
    <dbReference type="GeneID" id="60825228"/>
    <dbReference type="KEGG" id="rme:Rmet_0741"/>
    <dbReference type="eggNOG" id="COG1522">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_091233_0_0_4"/>
    <dbReference type="OMA" id="AGDENYI"/>
    <dbReference type="OrthoDB" id="1844486at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000485">
        <property type="entry name" value="AsnC-type_HTH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011008">
        <property type="entry name" value="Dimeric_a/b-barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019888">
        <property type="entry name" value="Tscrpt_reg_AsnC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019887">
        <property type="entry name" value="Tscrpt_reg_AsnC/Lrp_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019885">
        <property type="entry name" value="Tscrpt_reg_HTH_AsnC-type_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01037">
        <property type="entry name" value="AsnC_trans_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00033">
        <property type="entry name" value="HTHASNC"/>
    </dbReference>
    <dbReference type="SMART" id="SM00344">
        <property type="entry name" value="HTH_ASNC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54909">
        <property type="entry name" value="SSF54909"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00519">
        <property type="entry name" value="HTH_ASNC_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50956">
        <property type="entry name" value="HTH_ASNC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH asnC-type" evidence="4">
        <location>
            <begin position="11"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50956"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABF07627.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="169" mass="19114" checksum="E015E7F897D53606" modified="2006-05-30" version="1">MRTSRQPVRALDRLDRKILGVLQADGRISMKELAEAVGLTITPCIERVKRLERDGVIMGYYARLNPTLLGSALLVFVEISLGNKSGNMFEQFRREVLRIPEVLECHLVSGDFDYLIKARIREIGEYRRLLGDILLQLPGAAQSKSYVVMEEIKETLAIAVDEKERSQPG</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="69" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LQQ4</accession>
    <name>Q1LQQ4_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_0636</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07522.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515493.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0636"/>
    <dbReference type="EnsemblBacteria" id="ABF07522">
        <property type="protein sequence ID" value="ABF07522"/>
        <property type="gene ID" value="Rmet_0636"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0636"/>
    <dbReference type="eggNOG" id="COG3520">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_048238_2_0_4"/>
    <dbReference type="OMA" id="NRRVTYK"/>
    <dbReference type="OrthoDB" id="1688251at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010732">
        <property type="entry name" value="T6SS_TssG-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35564">
        <property type="entry name" value="PTHR35564"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06996">
        <property type="entry name" value="T6SS_TssG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03347">
        <property type="entry name" value="VI_chp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF07522.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="362" mass="39693" checksum="7832979FD028A6D8" modified="2006-05-30" version="1">MSPPKEGPLLRALLANAPQMNFFRLCELIELSVPQRPPLGTTDSLADDPVRFRSNGGLGFPGREIDLVDYDDDYPDRPPVIRTAFLGLYGVDARMPSYFVDEIAQRRDGAESLAAFLDLFHHRVVTQFYRVARKYRYPMGFLSGGPDEVSRYLLSLLGLGLREPGAGRPGVEHVEHVEGAVSPRKLLSMLGLASQRTRTAEGLAGVLQHAVPDAQILVEEFYPVWVRLDATERMPLGENCVLGRGFYDRANAVRVVITPQTPESVLGLMPGRAMHREVMVLLRFYLGHAAHAHLEMQVRPALMPAPALNSNAVSLGYSTQLQCSVATTNANATRGAAITRVQLGTWNGFNAGASAPLSHRSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="57" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LR63</accession>
    <name>Q1LR63_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_0477</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07363.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515350.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0477"/>
    <dbReference type="EnsemblBacteria" id="ABF07363">
        <property type="protein sequence ID" value="ABF07363"/>
        <property type="gene ID" value="Rmet_0477"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0477"/>
    <dbReference type="eggNOG" id="ENOG5030X9Q">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_136209_5_1_4"/>
    <dbReference type="OMA" id="GPRDVYT"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193450" evidence="1">
        <location>
            <begin position="22"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF07363.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="81" mass="8363" checksum="96B06CF8C5A3569A" modified="2006-05-30" version="1">MKTLLMLAAACAALGAGMAHAGNGPRDMYLDGARTPDPYTDGAKATRFDVYTDGASVLGTRDVFSDGSSTIDKRDAFTDGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="61" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LR65</accession>
    <name>Q1LR65_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_0475</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07361.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515348.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0475"/>
    <dbReference type="EnsemblBacteria" id="ABF07361">
        <property type="protein sequence ID" value="ABF07361"/>
        <property type="gene ID" value="Rmet_0475"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0475"/>
    <dbReference type="eggNOG" id="ENOG5032S0D">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_119887_1_0_4"/>
    <dbReference type="OMA" id="VPPDYYP"/>
    <dbReference type="OrthoDB" id="2008495at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193653" evidence="2">
        <location>
            <begin position="24"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="152"/>
            <end position="179"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF07361.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="179" mass="17943" checksum="93DAE1B704C91E69" modified="2006-05-30" version="1">MNLRTLSLLTLATTASLGLGACAVMPSGPTVMALPGSNKTFDQFRGDDYNCRQFAFQQIGGTTAQQNANAAAVGSTVVGTALGAAAGAAFGGGSGAAVGAGAGMLTGAAVGTGAAYNSAYGTQRQYDAAYVQCMYATGNRVPVYGQMVSAPPPGPAHEPMPPANYQPYYPPPPPPGYVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="102" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LR78</accession>
    <name>Q1LR78_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Permease of the Major Facilitator Superfamily</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Rmet_0462</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07348.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515337.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0462"/>
    <dbReference type="EnsemblBacteria" id="ABF07348">
        <property type="protein sequence ID" value="ABF07348"/>
        <property type="gene ID" value="Rmet_0462"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0462"/>
    <dbReference type="eggNOG" id="COG0477">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001265_39_2_4"/>
    <dbReference type="OMA" id="LWYPIVF"/>
    <dbReference type="OrthoDB" id="446363at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="SSF103473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00217">
        <property type="entry name" value="SUGAR_TRANSPORT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="26"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="64"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="122"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="164"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="197"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="252"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="290"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="319"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="465"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="511"/>
            <end position="529"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="535"/>
            <end position="553"/>
        </location>
    </feature>
    <feature type="domain" description="MFS" evidence="6">
        <location>
            <begin position="26"/>
            <end position="557"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABF07348.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="565" mass="61378" checksum="51219771DA170523" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-05-05" modified="2022-08-03" version="103" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LR87</accession>
    <name>MIAB_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">tRNA-2-methylthio-N(6)-dimethylallyladenosine synthase</fullName>
            <ecNumber evidence="1">2.8.4.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">(Dimethylallyl)adenosine tRNA methylthiotransferase MiaB</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">tRNA-i(6)A37 methylthiotransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">miaB</name>
        <name type="ordered locus">Rmet_0453</name>
    </gene>
    <organism>
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the methylthiolation of N6-(dimethylallyl)adenosine (i(6)A), leading to the formation of 2-methylthio-N6-(dimethylallyl)adenosine (ms(2)i(6)A) at position 37 in tRNAs that read codons beginning with uridine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>[sulfur carrier]-SH + AH2 + N(6)-dimethylallyladenosine(37) in tRNA + 2 S-adenosyl-L-methionine = 2-methylsulfanyl-N(6)-dimethylallyladenosine(37) in tRNA + 5'-deoxyadenosine + [sulfur carrier]-H + A + 2 H(+) + L-methionine + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:37067"/>
            <dbReference type="Rhea" id="RHEA-COMP:10375"/>
            <dbReference type="Rhea" id="RHEA-COMP:10376"/>
            <dbReference type="Rhea" id="RHEA-COMP:14737"/>
            <dbReference type="Rhea" id="RHEA-COMP:14739"/>
            <dbReference type="ChEBI" id="CHEBI:13193"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17319"/>
            <dbReference type="ChEBI" id="CHEBI:17499"/>
            <dbReference type="ChEBI" id="CHEBI:29917"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:64428"/>
            <dbReference type="ChEBI" id="CHEBI:74415"/>
            <dbReference type="ChEBI" id="CHEBI:74417"/>
            <dbReference type="EC" id="2.8.4.3"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="1">Binds 2 [4Fe-4S] clusters. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the methylthiotransferase family. MiaB subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.8.4.3" evidence="1"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07339.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515327.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q1LR87"/>
    <dbReference type="SMR" id="Q1LR87"/>
    <dbReference type="STRING" id="266264.Rmet_0453"/>
    <dbReference type="EnsemblBacteria" id="ABF07339">
        <property type="protein sequence ID" value="ABF07339"/>
        <property type="gene ID" value="Rmet_0453"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0453"/>
    <dbReference type="eggNOG" id="COG0621">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018697_2_0_4"/>
    <dbReference type="OMA" id="CEHFHIP"/>
    <dbReference type="OrthoDB" id="397139at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035596">
        <property type="term" value="F:methylthiotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006400">
        <property type="term" value="P:tRNA modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12160">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.30.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01864">
        <property type="entry name" value="tRNA_metthiotr_MiaB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaB/NifB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005839">
        <property type="entry name" value="Methylthiotransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020612">
        <property type="entry name" value="Methylthiotransferase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013848">
        <property type="entry name" value="Methylthiotransferase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038135">
        <property type="entry name" value="Methylthiotransferase_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006463">
        <property type="entry name" value="MiaB_methiolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023404">
        <property type="entry name" value="rSAM_horseshoe"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002792">
        <property type="entry name" value="TRAM_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01938">
        <property type="entry name" value="TRAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00919">
        <property type="entry name" value="UPF0004"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00273">
        <property type="entry name" value="(dimethylallyl)adenosine_tRNA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00089">
        <property type="entry name" value="TIGR00089"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51449">
        <property type="entry name" value="MTTASE_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01278">
        <property type="entry name" value="MTTASE_RADICAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50926">
        <property type="entry name" value="TRAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0004">4Fe-4S</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0408">Iron</keyword>
    <keyword id="KW-0411">Iron-sulfur</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <keyword id="KW-0819">tRNA processing</keyword>
    <feature type="chain" id="PRO_0000374477" description="tRNA-2-methylthio-N(6)-dimethylallyladenosine synthase">
        <location>
            <begin position="1"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="domain" description="MTTase N-terminal" evidence="1">
        <location>
            <begin position="2"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="domain" description="Radical SAM core" evidence="2">
        <location>
            <begin position="142"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="domain" description="TRAM" evidence="1">
        <location>
            <begin position="378"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="11"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="48"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="82"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="156"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="160"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="163"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01266"/>
        </source>
    </evidence>
    <sequence length="450" mass="50151" checksum="1BCA1E08211B509F" modified="2006-05-30" version="1">MKKVFVKTYGCQMNEYDSDKMVDVLNATQGMEPTDNVEDADVILFNTCSIREKAQEKVFSELGRMKALKAAKPDLVIGVGGCVASQEGASIVSRAPYVDVVFGPQTLHRLPDLINRRQQTGLSQVDISFPEIEKFDHLPPARVDGPSAFVSIMEGCSKYCSYCVVPYTRGEEVSRPFEDVLAEVAGLAEQGVREVTLLGQNVNAYRGAMGGTSEIADFALLIEYVADIPGIERIRYTTSHPKEFTSRLIELYGRCDKLVNHLHLPVQHASDRVLAGMKRGYSVLEYKSIIRRLRALRPDMSMSSDFIVGFPGETDADFDKLMAMVHEIGYDTSFSFIFSPRPGTPAANLHDDTPHEVKLRRLQLLQATIEENVQRISQSMVGTVQRILVEGPARKDPTELHGRTENNRVVNFALPGVPQAQRDRMIGQMVDVNVTQAFPHSLRGEFVVRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-12-02" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LRI0</accession>
    <name>Q1LRI0_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Carbon monoxide dehydrogenase accessory protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">coxG</name>
        <name evidence="2" type="ordered locus">Rmet_0360</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07246.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0360"/>
    <dbReference type="EnsemblBacteria" id="ABF07246">
        <property type="protein sequence ID" value="ABF07246"/>
        <property type="gene ID" value="Rmet_0360"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0360"/>
    <dbReference type="eggNOG" id="COG3427">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046420_1_0_4"/>
    <dbReference type="OMA" id="MELTQTH"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd05018">
        <property type="entry name" value="CoxG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.530.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010419">
        <property type="entry name" value="CO_DH_gsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023393">
        <property type="entry name" value="START-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38588">
        <property type="entry name" value="PTHR38588"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06240">
        <property type="entry name" value="COXG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="143"/>
            <end position="187"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF07246.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="187" mass="19755" checksum="92980224BDBE2031" modified="2006-05-30" version="1">MTQTQRLPVPQQVAWDALNDTALLKQCIPGCESIEPDGDNAYQLALTAAVGPVKARFKGRMALQDIQAPESYTIQFDGQGGAAGFGKGSAQVKLTPEGDETLLTYVVHAQVGGKIAQIGSRLVDAAARKMADTFFARFTEAVGGGGANEDDDNTETEQSADAGKTTDGGDDTGTKRKRSWTAWISKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LRL1</accession>
    <name>Q1LRL1_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Arsenate reductase</fullName>
            <ecNumber evidence="3">1.20.4.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">arsC1</name>
        <name evidence="4" type="ordered locus">Rmet_0329</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>[glutaredoxin]-dithiol + arsenate + glutathione + H(+) = arsenite + glutathionyl-S-S-[glutaredoxin] + H2O</text>
            <dbReference type="Rhea" id="RHEA:22016"/>
            <dbReference type="Rhea" id="RHEA-COMP:10729"/>
            <dbReference type="Rhea" id="RHEA-COMP:17668"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29242"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:48597"/>
            <dbReference type="ChEBI" id="CHEBI:57925"/>
            <dbReference type="ChEBI" id="CHEBI:146199"/>
            <dbReference type="EC" id="1.20.4.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 2 3">Belongs to the ArsC family.</text>
    </comment>
    <dbReference type="EC" id="1.20.4.1" evidence="3"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07215.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515210.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0329"/>
    <dbReference type="EnsemblBacteria" id="ABF07215">
        <property type="protein sequence ID" value="ABF07215"/>
        <property type="gene ID" value="Rmet_0329"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0329"/>
    <dbReference type="eggNOG" id="COG1393">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_116644_0_0_4"/>
    <dbReference type="OMA" id="HLEKHNS"/>
    <dbReference type="OrthoDB" id="1650947at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008794">
        <property type="term" value="F:arsenate reductase (glutaredoxin) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd03034">
        <property type="entry name" value="ArsC_ArsC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006659">
        <property type="entry name" value="Arsenate_reductase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006660">
        <property type="entry name" value="Arsenate_reductase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30041">
        <property type="entry name" value="PTHR30041"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03960">
        <property type="entry name" value="ArsC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="SSF52833"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00014">
        <property type="entry name" value="arsC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51353">
        <property type="entry name" value="ARSC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU362029"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF07215.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="140" mass="15361" checksum="2D515E04731D12D7" modified="2006-05-30" version="1">MTVTIYHNPACGTSRNTLAMIRNAGIEPIVVEYLNMPPDRQTLQSMIRAAGLSVREAIREKGTPYAELGLGDPALTDDQLLDAMLAHPILINRPFVITELGVRLCRPSEVVLDILPAPQQGAFTKEDGEAVIDTQGRRIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="110" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LRU4</accession>
    <name>Q1LRU4_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 13">Phosphoenolpyruvate-protein phosphotransferase</fullName>
            <ecNumber evidence="5 13">2.7.3.9</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12 13">Phosphotransferase system, enzyme I</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="21" type="primary">ptsI</name>
        <name evidence="21" type="ordered locus">Rmet_0246</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="22">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">General (non sugar-specific) component of the phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS). This major carbohydrate active-transport system catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. Enzyme I transfers the phosphoryl group from phosphoenolpyruvate (PEP) to the phosphoryl carrier protein (HPr).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 13">
            <text>L-histidyl-[protein] + phosphoenolpyruvate = N(pros)-phospho-L-histidyl-[protein] + pyruvate</text>
            <dbReference type="Rhea" id="RHEA:23880"/>
            <dbReference type="Rhea" id="RHEA-COMP:9745"/>
            <dbReference type="Rhea" id="RHEA-COMP:9746"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:29979"/>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
            <dbReference type="ChEBI" id="CHEBI:64837"/>
            <dbReference type="EC" id="2.7.3.9"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 13 16">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3 13">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 13">Belongs to the PEP-utilizing enzyme family.</text>
    </comment>
    <dbReference type="EC" id="2.7.3.9" evidence="5 13"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07132.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515142.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0246"/>
    <dbReference type="EnsemblBacteria" id="ABF07132">
        <property type="protein sequence ID" value="ABF07132"/>
        <property type="gene ID" value="Rmet_0246"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0246"/>
    <dbReference type="eggNOG" id="COG1080">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007308_7_0_4"/>
    <dbReference type="OMA" id="CNAEWAL"/>
    <dbReference type="OrthoDB" id="467393at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008965">
        <property type="term" value="F:phosphoenolpyruvate-protein phosphotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009401">
        <property type="term" value="P:phosphoenolpyruvate-dependent sugar phosphotransferase system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.274.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008279">
        <property type="entry name" value="PEP-util_enz_mobile_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018274">
        <property type="entry name" value="PEP_util_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000121">
        <property type="entry name" value="PEP_util_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023151">
        <property type="entry name" value="PEP_util_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036637">
        <property type="entry name" value="Phosphohistidine_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024692">
        <property type="entry name" value="PTS_EI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006318">
        <property type="entry name" value="PTS_EI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008731">
        <property type="entry name" value="PTS_EIN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036618">
        <property type="entry name" value="PtsI_HPr-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015813">
        <property type="entry name" value="Pyrv/PenolPyrv_Kinase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040442">
        <property type="entry name" value="Pyrv_Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05524">
        <property type="entry name" value="PEP-utilisers_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00391">
        <property type="entry name" value="PEP-utilizers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02896">
        <property type="entry name" value="PEP-utilizers_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000732">
        <property type="entry name" value="PTS_enzyme_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47831">
        <property type="entry name" value="SSF47831"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51621">
        <property type="entry name" value="SSF51621"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52009">
        <property type="entry name" value="SSF52009"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01417">
        <property type="entry name" value="PTS_I_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00742">
        <property type="entry name" value="PEP_ENZYMES_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00370">
        <property type="entry name" value="PEP_ENZYMES_PHOS_SITE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="17" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8 13" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="13" id="KW-0418">Kinase</keyword>
    <keyword evidence="13 16" id="KW-0460">Magnesium</keyword>
    <keyword evidence="11 13 16" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10 13" id="KW-0598">Phosphotransferase system</keyword>
    <keyword evidence="21" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 13" id="KW-0762">Sugar transport</keyword>
    <keyword evidence="13 21" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 13" id="KW-0813">Transport</keyword>
    <feature type="domain" description="PEP-utilisers_N" evidence="20">
        <location>
            <begin position="6"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="domain" description="PEP-utilizers" evidence="18">
        <location>
            <begin position="165"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="domain" description="PEP-utilizers_C" evidence="19">
        <location>
            <begin position="263"/>
            <end position="556"/>
        </location>
    </feature>
    <feature type="region of interest" description="PEP binding" evidence="15">
        <location>
            <begin position="469"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="17">
        <location>
            <begin position="36"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="active site" description="Tele-phosphohistidine intermediate" evidence="14">
        <location>
            <position position="201"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="14">
        <location>
            <position position="517"/>
        </location>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="308"/>
        </location>
        <ligand>
            <name>phosphoenolpyruvate</name>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="345"/>
        </location>
        <ligand>
            <name>phosphoenolpyruvate</name>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="446"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="470"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="480"/>
        </location>
        <ligand>
            <name>phosphoenolpyruvate</name>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000683"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012232"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00016544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022597"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022683"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00033235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRNR" id="PIRNR000732"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR000732-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR000732-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR000732-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF00391"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF02896"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF05524"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="ABF07132.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="585" mass="64537" checksum="E77A55289D45C39D" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="104" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LRU7</accession>
    <name>Q1LRU7_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Glutathione synthetase</fullName>
            <ecNumber evidence="10">6.3.2.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">GSH synthetase</fullName>
            <shortName evidence="10">GSH-S</shortName>
            <shortName evidence="10">GSHase</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">Glutathione synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10 13" type="primary">gshB</name>
        <name evidence="13" type="ordered locus">Rmet_0243</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>ATP + gamma-L-glutamyl-L-cysteine + glycine = ADP + glutathione + H(+) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:13557"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
            <dbReference type="ChEBI" id="CHEBI:57925"/>
            <dbReference type="ChEBI" id="CHEBI:58173"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.2.3"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="10">Sulfur metabolism; glutathione biosynthesis; glutathione from L-cysteine and L-glutamate: step 2/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the prokaryotic GSH synthase family.</text>
    </comment>
    <dbReference type="EC" id="6.3.2.3" evidence="10"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07129.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515141.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0243"/>
    <dbReference type="EnsemblBacteria" id="ABF07129">
        <property type="protein sequence ID" value="ABF07129"/>
        <property type="gene ID" value="Rmet_0243"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0243"/>
    <dbReference type="eggNOG" id="COG0189">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_068239_0_0_4"/>
    <dbReference type="OMA" id="WMRKDPP"/>
    <dbReference type="OrthoDB" id="878336at2"/>
    <dbReference type="UniPathway" id="UPA00142">
        <property type="reaction ID" value="UER00210"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004363">
        <property type="term" value="F:glutathione synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00162">
        <property type="entry name" value="GSH_S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011761">
        <property type="entry name" value="ATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013815">
        <property type="entry name" value="ATP_grasp_subdomain_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006284">
        <property type="entry name" value="Glut_synth_pro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004218">
        <property type="entry name" value="GSHS_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004215">
        <property type="entry name" value="GSHS_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016185">
        <property type="entry name" value="PreATP-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21621:SF4">
        <property type="entry name" value="PTHR21621:SF4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02955">
        <property type="entry name" value="GSH-S_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02951">
        <property type="entry name" value="GSH-S_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52440">
        <property type="entry name" value="SSF52440"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01380">
        <property type="entry name" value="glut_syn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50975">
        <property type="entry name" value="ATP_GRASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 10" id="KW-0317">Glutathione biosynthesis</keyword>
    <keyword evidence="3 10 13" id="KW-0436">Ligase</keyword>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="9" id="KW-0464">Manganese</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 10 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ATP-grasp" evidence="12">
        <location>
            <begin position="124"/>
            <end position="309"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ABF07129.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="317" mass="34790" checksum="2E251B06662FF180" modified="2006-05-30" version="1">MRILFITDPLETFKTYKDSTFAMMTEAAARGHELFFCLQSQLSLSANVVEAMVRPLELTDDAQAWYRLGDAALMPLSAFDAVLMRKDPPFDMEYVTSTWLLELAEAQGARVFNKPRAIRDHSEKLAIGQHPQFITPTLVTRDAGRIREFHAEHKDIIVKPLDGMGGMGVFRVGADAMNLGAIVETLGDNGARTLMVQRYIPAIKDGDKRILLIGGKPVPYSLARIPQGTEIRGNLAAGGVGRAQELSARDREIAETLAPGLWKQGLLLVGLDVIGDYLTEVNVTSPTCFQEIAQQTGFNVAGMFIDALEQAVAADRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LS13</accession>
    <name>Q1LS13_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative NAD-dependent epimerase/dehydratase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_0177</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07063.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515086.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0177"/>
    <dbReference type="EnsemblBacteria" id="ABF07063">
        <property type="protein sequence ID" value="ABF07063"/>
        <property type="gene ID" value="Rmet_0177"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0177"/>
    <dbReference type="eggNOG" id="COG0702">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007383_6_5_4"/>
    <dbReference type="OMA" id="FFNRFAA"/>
    <dbReference type="OrthoDB" id="1928091at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001509">
        <property type="entry name" value="Epimerase_deHydtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01370">
        <property type="entry name" value="Epimerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Epimerase" evidence="1">
        <location>
            <begin position="6"/>
            <end position="237"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF07063.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="340" mass="36308" checksum="9608D27397ADC318" modified="2006-05-30" version="1">MQTSNVLVIGGAGFIGSHLISRLAGAATASGAPLDPASHPDSPIAPERIIVGTRNVEHAQHLLLLPRVEVIELGLADDAVLDDAIGSLGVDGIVVNLVGVLHGERGDPYGPQFAAAHVDLPRQVVESCKRTGVRRLLHMSALGADPQGPSMYLRSKGDGGRIVRESGLDWTIFRPSVVFGPDDHFLNLFAQLQQMAPVVPLACAHARFQPVFVLDVVQAFVNAMVHPETIGQVYELGGPQVYTLEELVRFAGRASGHPRPVIALPDALARMQAAVLEHVPGGTVLSRDNLDSMRLDNVLAHPMAAELGVRPVSLEVVMTDVLSGRNRDSALQFMRGSVHR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="101" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LS18</accession>
    <name>Q1LS18_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Methylenetetrahydrofolate reductase</fullName>
            <ecNumber evidence="10">1.5.1.20</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">metF</name>
        <name evidence="11" type="ordered locus">Rmet_0172</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="2 10">
            <text>(6S)-5-methyl-5,6,7,8-tetrahydrofolate + NAD(+) = (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate + H(+) + NADH</text>
            <dbReference type="Rhea" id="RHEA:19821"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15636"/>
            <dbReference type="ChEBI" id="CHEBI:18608"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.5.1.20"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 10">
            <text>(6S)-5-methyl-5,6,7,8-tetrahydrofolate + NADP(+) = (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate + H(+) + NADPH</text>
            <dbReference type="Rhea" id="RHEA:19817"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15636"/>
            <dbReference type="ChEBI" id="CHEBI:18608"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.5.1.20"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3 10">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="4 10">One-carbon metabolism; tetrahydrofolate interconversion.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5 10">Belongs to the methylenetetrahydrofolate reductase family.</text>
    </comment>
    <dbReference type="EC" id="1.5.1.20" evidence="10"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07058.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515081.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0172"/>
    <dbReference type="EnsemblBacteria" id="ABF07058">
        <property type="protein sequence ID" value="ABF07058"/>
        <property type="gene ID" value="Rmet_0172"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0172"/>
    <dbReference type="eggNOG" id="COG0685">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025841_0_0_4"/>
    <dbReference type="OMA" id="EMHPQAR"/>
    <dbReference type="OrthoDB" id="1425269at2"/>
    <dbReference type="UniPathway" id="UPA00193"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106312">
        <property type="term" value="F:methylenetetrahydrofolate reductase NADH activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106313">
        <property type="term" value="F:methylenetetrahydrofolate reductase NADPH activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009086">
        <property type="term" value="P:methionine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035999">
        <property type="term" value="P:tetrahydrofolate interconversion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00537">
        <property type="entry name" value="MTHFR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029041">
        <property type="entry name" value="FAD-linked_oxidoreductase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003171">
        <property type="entry name" value="Mehydrof_redctse-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004620">
        <property type="entry name" value="MTHF_reductase_bac"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02219">
        <property type="entry name" value="MTHFR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51730">
        <property type="entry name" value="SSF51730"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00676">
        <property type="entry name" value="fadh2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0274">FAD</keyword>
    <keyword evidence="6 10" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="9" id="KW-0520">NAD</keyword>
    <keyword evidence="8 10 11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000431"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001287"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00006743"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU003862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ABF07058.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="276" mass="30897" checksum="61FC70654E9FA65B" modified="2006-05-30" version="1">MTDRYFSFEFFPPKTAEGAEKLRNTRAQLAPLQPKYISVTFGAGGTTQQGTLDAVLEIQREGIEAAPHLSCVGSTRDSIRQILQTYRDGGIRHIVALRGDMPSGMGEIGEFRYANELVEFIRAETGDWFNIEVAAYPEYHPQARSPRHDLENFVRKVKAGADSAITQYFFNADAYFRFVDDVRAMGVEVPIVPGIMPITNYSQLMRFSEMCGAEVPRWVAKRLESFGDDKEAIRAFGLDVVTDLCTRLLEAGVPGLHFYTLNAAGATKAIWQRLKL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-01-15" modified="2022-08-03" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LS37</accession>
    <name>DAPF_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Diaminopimelate epimerase</fullName>
            <shortName evidence="1">DAP epimerase</shortName>
            <ecNumber evidence="1">5.1.1.7</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">PLP-independent amino acid racemase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">dapF</name>
        <name type="ordered locus">Rmet_0153</name>
    </gene>
    <organism>
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the stereoinversion of LL-2,6-diaminoheptanedioate (L,L-DAP) to meso-diaminoheptanedioate (meso-DAP), a precursor of L-lysine and an essential component of the bacterial peptidoglycan.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(2S,6S)-2,6-diaminoheptanedioate = meso-2,6-diaminoheptanedioate</text>
            <dbReference type="Rhea" id="RHEA:15393"/>
            <dbReference type="ChEBI" id="CHEBI:57609"/>
            <dbReference type="ChEBI" id="CHEBI:57791"/>
            <dbReference type="EC" id="5.1.1.7"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-lysine biosynthesis via DAP pathway; DL-2,6-diaminopimelate from LL-2,6-diaminopimelate: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the diaminopimelate epimerase family.</text>
    </comment>
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        <property type="protein sequence ID" value="ABF07039.1"/>
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    <dbReference type="SMR" id="Q1LS37"/>
    <dbReference type="STRING" id="266264.Rmet_0153"/>
    <dbReference type="PRIDE" id="Q1LS37"/>
    <dbReference type="EnsemblBacteria" id="ABF07039">
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        <property type="gene ID" value="Rmet_0153"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0153"/>
    <dbReference type="eggNOG" id="COG0253">
        <property type="taxonomic scope" value="Bacteria"/>
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    <dbReference type="HOGENOM" id="CLU_053306_1_1_4"/>
    <dbReference type="OMA" id="HVAMRVH"/>
    <dbReference type="OrthoDB" id="1921631at2"/>
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        <property type="reaction ID" value="UER00025"/>
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    <dbReference type="Proteomes" id="UP000002429">
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    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="UniProtKB-UniRule"/>
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        <property type="term" value="P:lysine biosynthetic process via diaminopimelate"/>
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        <property type="match status" value="1"/>
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            <begin position="1"/>
            <end position="288"/>
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            <position position="75"/>
        </location>
    </feature>
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            <position position="226"/>
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        <location>
            <position position="13"/>
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        <ligand>
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        <ligand>
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        <ligand>
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    <feature type="binding site" evidence="1">
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        <ligand>
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        </location>
        <ligand>
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    <feature type="binding site" evidence="1">
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        <ligand>
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        </location>
        <ligand>
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        </ligand>
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        <location>
            <position position="168"/>
        </location>
    </feature>
    <feature type="site" description="Could be important to modulate the pK values of the two catalytic cysteine residues" evidence="1">
        <location>
            <position position="217"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00197"/>
        </source>
    </evidence>
    <sequence length="288" mass="31294" checksum="F58389E943A2B99A" modified="2006-05-30" version="1">MKLQFTKMHGAGNDFIVLDGIHQKLDLTDAQWRALANRHFGIGADQILIVEKSSRDDVDFRYRIVNADGGEVEHCGNGARCFVRFVTDRGMTDKQSVRVEVMNGVITLKLQDDGQVTVDMGEPELTPARVPFIADGLPTRAEAQDTLYGLEVNGRTEWISPVSMGNPHAVQIVDDVEQFPVLQDGPVIEHHKSFPNRVNAGFMQIVDRNTVRLRVFERGAGETLACGTGACAAVVAGIRRGLLDSPVKVHTHGGDLTIAWQGAGQPVQMTGPATTVFEGTIDLSTLPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="67" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LS45</accession>
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        </submittedName>
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    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
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                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="ABF07031"/>
        <property type="gene ID" value="Rmet_0145"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Acyl-CoA_hotdog"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029069">
        <property type="entry name" value="HotDog_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54637">
        <property type="entry name" value="SSF54637"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF07031.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="272" mass="29602" checksum="B109F625AE1E23E0" modified="2006-05-30" version="1">MTQAPLHPLDQALKLEPAGEHRYVGHTSQAYWNMIGPFGGATAAVMLQAALVHPERLGDPIALTVNFAGPIGEGDFEVEARPSRTNRSTQHWHLELRQNGEVTTTGSAVFAVRRQTWNSGEAVMPQVPAAESLPVMSGFAPVKWLGSYDIRAVRGAKPTTAPGTENPDSLSQFWIRDEPARTPDYASMAAWCDAFYPRIFLKRAGFVPAGTVSLTTYFHADAATLAAVGDSHVLGCAQARVFRQGFFDQSAEIWSASGELLATSHQIVYYKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-10-07" version="51" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LS51</accession>
    <name>Q1LS51_CUPMC</name>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_0139</name>
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    <organism evidence="2 3">
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        <lineage>
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        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
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        <property type="gene ID" value="Rmet_0139"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Cys_rich_CWC"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14375">
        <property type="entry name" value="Cys_rich_CWC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF07025.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="102" mass="11180" checksum="93D053B890F48D84" modified="2006-05-30" version="1">MRHPATAGADFTRNPRPHCNPDEWHTMTDPSTIQTGQLRPVEHCSRCGAAFVCGYVAGLPECWCASLPLLPSRKIVAGQHCLCPTCLRQRQDDVPQDAVAPD</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LSI4</accession>
    <name>Q1LSI4_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Transmembrane transport protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_0006</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
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        <lineage>
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            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
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        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF06892.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0006"/>
    <dbReference type="EnsemblBacteria" id="ABF06892">
        <property type="protein sequence ID" value="ABF06892"/>
        <property type="gene ID" value="Rmet_0006"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0006"/>
    <dbReference type="eggNOG" id="COG0697">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033863_4_3_4"/>
    <dbReference type="OMA" id="GFLLWYA"/>
    <dbReference type="OrthoDB" id="1515911at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000620">
        <property type="entry name" value="EamA_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00892">
        <property type="entry name" value="EamA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="35"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="68"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="100"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="124"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="148"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="181"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="213"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="245"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="270"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="domain" description="EamA" evidence="2">
        <location>
            <begin position="7"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="domain" description="EamA" evidence="2">
        <location>
            <begin position="152"/>
            <end position="285"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF06892.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="309" mass="31702" checksum="F4F61E4F975A2863" modified="2006-05-30" version="1">MSRRMHGYLYLTLAMITVGSTVVASKIVAAGLPPFTATALRFAIALPLFLVLMRVTRITWPILAPREWGLLLVQAGAGSVGYTTLLILGLRHASATDAGVIIGTLPVVSAAIAIMVLGERPRRAVLLAIALAAAGVLTIVFRNDAGSAHSLLGSLLIFGAVGCEGVFILLNKRIQTPIAPLALSTIMAVFGLAVAVIPAGFEMPWMHALSGNAVAAVAYYALIPTVGGFLLWYAGAARVSGAEAALFTAVAPVSAVVLAAALLGEQIGQSQIIGIGCVLVAVFSLGMSQSADMTDSAMADAEGDATSHR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-03-23" modified="2020-10-07" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>D3DXR0</accession>
    <name>D3DXR0_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_6469</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ADC45080.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_6469"/>
    <dbReference type="EnsemblBacteria" id="ADC45080">
        <property type="protein sequence ID" value="ADC45080"/>
        <property type="gene ID" value="Rmet_6469"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6469"/>
    <dbReference type="HOGENOM" id="CLU_2828117_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADC45080.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="66" mass="7903" checksum="D74BC37F0A9D7B7C" modified="2010-03-23" version="1">MILIYLLLILAFHALINFAQMQYFNVYFSCLIVNKHILENVARFSPHWTWLYPRLCPPLEGIFFAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-03-23" modified="2020-10-07" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>D3DXU4</accession>
    <name>D3DXU4_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_6506</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ADC45114.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_6506"/>
    <dbReference type="EnsemblBacteria" id="ADC45114">
        <property type="protein sequence ID" value="ADC45114"/>
        <property type="gene ID" value="Rmet_6506"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6506"/>
    <dbReference type="HOGENOM" id="CLU_3405040_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADC45114.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="30" mass="3375" checksum="0743E3E2C1ED3505" modified="2010-03-23" version="1">MWQARKMSTQGAVVVPWGCTYMVPNFVVST</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-03-23" modified="2020-12-02" version="51" xmlns="http://uniprot.org/uniprot">
    <accession>D3DXV9</accession>
    <name>D3DXV9_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Mercuric reductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">merA''</name>
        <name evidence="5" type="ordered locus">Rmet_2315</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ADC45129.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2315"/>
    <dbReference type="EnsemblBacteria" id="ADC45129">
        <property type="protein sequence ID" value="ADC45129"/>
        <property type="gene ID" value="Rmet_2315"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2315"/>
    <dbReference type="eggNOG" id="COG1249">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016755_3_6_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045454">
        <property type="term" value="P:cell redox homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.390.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023753">
        <property type="entry name" value="FAD/NAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016156">
        <property type="entry name" value="FAD/NAD-linked_Rdtase_dimer_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004099">
        <property type="entry name" value="Pyr_nucl-diS_OxRdtase_dimer"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07992">
        <property type="entry name" value="Pyr_redox_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02852">
        <property type="entry name" value="Pyr_redox_dim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="SSF51905"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55424">
        <property type="entry name" value="SSF55424"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pyr_redox_2" evidence="4">
        <location>
            <begin position="34"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="domain" description="Pyr_redox_dim" evidence="3">
        <location>
            <begin position="161"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF02852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF07992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ADC45129.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="285" mass="30040" checksum="07EEDD55D3CB1EB1" modified="2010-03-23" version="1">MVPPWWRSSWRKPSPGGQPGHDPGAQHAVLPRRPAIGEAVTDAFRAEGIEVLDHTQASHVAYAGGEFVLTTGQGEVRADKLLVATGRAPNTRSLNLEAAGVEVNAQGAIVIDRAMRTSAPHIFAAGDCTDQPQFVYVAAAAGTRAAINMTGGDAALDLTAMPAVVFTDPQVATVGYSEAEAHHDGIETDSRLLTLDNVPRALANFDTRGFIKLVAEAGSGRLIGVQAVAPEAGELIQTAALAIRHRMTVQELADQLFPYLTMVEGLKLAAQTFNKDVKQLSCCAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-03-23" modified="2020-10-07" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>D3DXY8</accession>
    <name>D3DXY8_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_6551</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ADC45158.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_6551"/>
    <dbReference type="EnsemblBacteria" id="ADC45158">
        <property type="protein sequence ID" value="ADC45158"/>
        <property type="gene ID" value="Rmet_6551"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6551"/>
    <dbReference type="HOGENOM" id="CLU_2828120_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003043436" evidence="1">
        <location>
            <begin position="25"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADC45158.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="66" mass="7204" checksum="1D07792017EDC412" modified="2010-03-23" version="1">MPRAFLLFRSIAGRLLFPWSDAIAAANAVAVGLDERHVTSYNFSRLPAPVQVQNAAPHPPSLRFSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-03-23" modified="2020-10-07" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>D3DY92</accession>
    <name>D3DY92_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_6668</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ADC45262.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADC45262">
        <property type="protein sequence ID" value="ADC45262"/>
        <property type="gene ID" value="Rmet_6668"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6668"/>
    <dbReference type="HOGENOM" id="CLU_3421144_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADC45262.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="24" mass="2918" checksum="6B294F6DDA8186D9" modified="2010-03-23" version="1">MSQMGHYYLWLTHTILRIIIQTAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-03-23" modified="2021-06-02" version="42" xmlns="http://uniprot.org/uniprot">
    <accession>D3DY94</accession>
    <name>D3DY94_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_6676</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ADC45264.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519059.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_6676"/>
    <dbReference type="EnsemblBacteria" id="ADC45264">
        <property type="protein sequence ID" value="ADC45264"/>
        <property type="gene ID" value="Rmet_6676"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6676"/>
    <dbReference type="HOGENOM" id="CLU_2156270_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003042618" evidence="2">
        <location>
            <begin position="27"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="70"/>
            <end position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ADC45264.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="111" mass="11445" checksum="30F2360F3FD06F36" modified="2010-03-23" version="1">MINHCRPAVRVFGIALLIAGAGHAYAQLASDMASPALPDWSHVCQGAGVAAKPAGCHASDAPAGSKYNLLYANDTSEGDEGEAPYLPANQPPRHAHEDASRDAPRDNASGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-03-23" modified="2020-10-07" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>D3DYE0</accession>
    <name>D3DYE0_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_6731</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ADC45310.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_6731"/>
    <dbReference type="EnsemblBacteria" id="ADC45310">
        <property type="protein sequence ID" value="ADC45310"/>
        <property type="gene ID" value="Rmet_6731"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6731"/>
    <dbReference type="HOGENOM" id="CLU_2411061_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADC45310.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="92" mass="10038" checksum="93BFF1B5E12D87DB" modified="2010-03-23" version="1">MTPNSILDRVDPLRQADLARRKIERSTSFTTECYVGLAGPLRDTALSEAFLIGWERIRSVAARSRSAPSEGRIVLTSRDFTVSDVAGRVGGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="59" xmlns="http://uniprot.org/uniprot">
    <accession>Q1L9N6</accession>
    <name>Q1L9N6_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_6281</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3 4">
        <name>pMOL28</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid pMOL28</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000355">
        <property type="protein sequence ID" value="ABF13140.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011514908.1">
        <property type="nucleotide sequence ID" value="NC_007972.2"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF13140">
        <property type="protein sequence ID" value="ABF13140"/>
        <property type="gene ID" value="Rmet_6281"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6281"/>
    <dbReference type="HOGENOM" id="CLU_2275146_0_0_4"/>
    <dbReference type="OMA" id="TQYERSA"/>
    <dbReference type="OrthoDB" id="2020070at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid pMOL28"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="20"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="57"/>
            <end position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF13140.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="102" mass="11358" checksum="F5DEDF80CB9330C4" modified="2006-05-30" version="1">MESFANNLIKRPLKLLAWSLALFSASQSFIVAMFVSAVLFFYLKEKAVQWSKVVPAAPKVSTPDNPAERKATPVQPAQPPETQYERSAVVTPIRRTGTHDRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LAN6</accession>
    <name>Q1LAN6_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Polysaccharide deacetylase family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_5931</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12790.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518136.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5931"/>
    <dbReference type="EnsemblBacteria" id="ABF12790">
        <property type="protein sequence ID" value="ABF12790"/>
        <property type="gene ID" value="Rmet_5931"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5931"/>
    <dbReference type="eggNOG" id="COG0726">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_076306_0_0_4"/>
    <dbReference type="OMA" id="RPPFWNY"/>
    <dbReference type="OrthoDB" id="1701876at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016810">
        <property type="term" value="F:hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011330">
        <property type="entry name" value="Glyco_hydro/deAcase_b/a-brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002509">
        <property type="entry name" value="NODB_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01522">
        <property type="entry name" value="Polysacc_deac_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88713">
        <property type="entry name" value="SSF88713"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51677">
        <property type="entry name" value="NODB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193261" evidence="1">
        <location>
            <begin position="26"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="domain" description="NodB homology" evidence="2">
        <location>
            <begin position="53"/>
            <end position="269"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF12790.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="299" mass="31858" checksum="74CCF7E94CB8C915" modified="2006-05-30" version="1">MRRLTLAAIATVTVIAALVVPPALAEGTVTAADADQPAALTKAPDAAQALAPVRFLLTFDDGPDSGPDGSTPVILRQLANNPIAPGIKAVFFVQTAHRRRGGSPEGQAQMRDTCAAGHLLAVHSGLPEGHVPHTRLTPEKLADSLRSGEADIVAQCGHVAPVVRPPDWAHNDATEAVYHQLGLDMLMADANARDGKIYGWHISLRRRSHMVSELERVGRAMTTHRLEPVDGVVPVIVAFHDTNDFTASHMTDYLQILVDAARENGLPLADPPFYTDTGAAEQAAVARAKAGLYARESWP</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="69" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LAW3</accession>
    <name>Q1LAW3_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative glycosyl transferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_5854</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12713.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518061.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5854"/>
    <dbReference type="CAZy" id="GT4">
        <property type="family name" value="Glycosyltransferase Family 4"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF12713">
        <property type="protein sequence ID" value="ABF12713"/>
        <property type="gene ID" value="Rmet_5854"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5854"/>
    <dbReference type="eggNOG" id="COG0438">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009583_5_2_4"/>
    <dbReference type="OMA" id="AVFCDST"/>
    <dbReference type="OrthoDB" id="635734at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001296">
        <property type="entry name" value="Glyco_trans_1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00534">
        <property type="entry name" value="Glycos_transf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glycos_transf_1" evidence="1">
        <location>
            <begin position="191"/>
            <end position="338"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF12713.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="381" mass="42667" checksum="D4935EF87D12D358" modified="2006-05-30" version="1">MIDIYHNILWPRYKGAVFSEAFSHAHKAGHKVRFFHISSTGYGRTAYSGVDTSYHRYPYEILFDEPYEAIPTWKMSLRLMREVWRSKNRLILMPGYHRVEFWAMLVACMLSGKRRAVFCDSTIQDQADHPMKRRLKRFFFSRCDGVFVYGRRSAEYAIAMGADPTKVICHCQAAALPHSYNAAEANRIRIANAPPAEAPRFLFAGLVCRAKGIEDLLRAFVEVRRQLPAATLVLAGPVVAADQLPELARSLGLGDAAQFLGAMDIDQLAQQYSRATCLVLPSHTEAWGLVVNEALNYGCPAVVSDCCGCIPELIKEGRTGHTFVTRDVDDLARVLLKATSSFADVQATMRNCVDVVRKYTPARAGRSIIKGCQKILKRART</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LB89</accession>
    <name>Q1LB89_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative glycosyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_5728</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12587.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5728"/>
    <dbReference type="CAZy" id="GT4">
        <property type="family name" value="Glycosyltransferase Family 4"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF12587">
        <property type="protein sequence ID" value="ABF12587"/>
        <property type="gene ID" value="Rmet_5728"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5728"/>
    <dbReference type="eggNOG" id="COG0438">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_075005_0_0_4"/>
    <dbReference type="OMA" id="HYHRPHL"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001296">
        <property type="entry name" value="Glyco_trans_1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00534">
        <property type="entry name" value="Glycos_transf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glycos_transf_1" evidence="1">
        <location>
            <begin position="160"/>
            <end position="293"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF12587.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="332" mass="36601" checksum="FABABA29E119D515" modified="2006-05-30" version="1">MLLVNHVMSNDVASGIFSDIISYYRSYAPPGIEHVASASAALGGMIRHYHRPNLESRLSGPCVVTVHHDLRDDDPSLTVQHFTDRYREANRVICLNSLQRDYLAADGITNTVVIPHGYHARYIRARGDATDTPYLENTGSGKNGRVVIGVFSRRYPRKVKGEGYIYELAQRLDPARFEFVLIGKGRTYDAAVLERFGFDVNAYEALPYKTVASAYRTVDALLMSSWFEGGPANIPEAVGGAVPVLCNPIGMAKDMVVDRENGLHLSMDAGSDAERISAWLLDPASRADLREGAMRNAGNAITWAIHASRVVDVYREVLNETLGSILTWQNAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="69" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LBH7</accession>
    <name>Q1LBH7_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_5640</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12499.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011520043.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5640"/>
    <dbReference type="EnsemblBacteria" id="ABF12499">
        <property type="protein sequence ID" value="ABF12499"/>
        <property type="gene ID" value="Rmet_5640"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5640"/>
    <dbReference type="eggNOG" id="COG3295">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_114004_0_0_4"/>
    <dbReference type="OMA" id="GFTLNHA"/>
    <dbReference type="OrthoDB" id="1921658at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032307">
        <property type="entry name" value="PepSY_TM-like_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40115">
        <property type="entry name" value="PTHR40115"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16357">
        <property type="entry name" value="PepSY_TM_like_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="23"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="173"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="201"/>
            <end position="222"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF12499.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="223" mass="24651" checksum="B6ABBC19AE4A4C8E" modified="2006-05-30" version="1">MTESTPASSQQRRAFWLKHLHQWHWISSAICLIGMLMFAVTGFTLNHAGQIEASPSVVTRHETAPRELLDALKRAAPANALAGNQKDQKDQKGPVPRVLADWLSKTMDIEAAGREAEWSADEIYVGLPRPGGDAWLSVALEDGEVQYEVTSRGWISYFNDLHKGRNTGHAWSWFIDIFALACLIFSVTGLFLLKLHAGGRMATWPLVGAGLVVPLLLAILFIH</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LBT0</accession>
    <name>Q1LBT0_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Dehydratase, MaoC-like domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_5537</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12396.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519942.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5537"/>
    <dbReference type="EnsemblBacteria" id="ABF12396">
        <property type="protein sequence ID" value="ABF12396"/>
        <property type="gene ID" value="Rmet_5537"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5537"/>
    <dbReference type="eggNOG" id="COG2030">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_094876_0_0_4"/>
    <dbReference type="OMA" id="DWTELHT"/>
    <dbReference type="OrthoDB" id="1563802at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029069">
        <property type="entry name" value="HotDog_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002539">
        <property type="entry name" value="MaoC-like_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01575">
        <property type="entry name" value="MaoC_dehydratas"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54637">
        <property type="entry name" value="SSF54637"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MaoC-like" evidence="1">
        <location>
            <begin position="19"/>
            <end position="130"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01575"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF12396.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="157" mass="17550" checksum="0A1E0683C2506064" modified="2006-05-30" version="1">MQEIEERKPRGMYFEDFEIGKTIVTSRRTVTLTDIVNFACLSGDHNAPHIDHEFCKTQSYGEPIAHGPLVLAIAGGLQCLSGINDGTIIAMLGNDQWRMHLPVKHGDTIHVVITPIEKKQSSKGTSGIVTFDRKVKNQRGEVVHSMITTLLYRLRPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LBZ3</accession>
    <name>Q1LBZ3_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Extra-cytoplasmic solute receptor protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">bug</name>
        <name evidence="4" type="ordered locus">Rmet_5474</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="5">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="5">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Periplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the UPF0065 (bug) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12333.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519879.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5474"/>
    <dbReference type="EnsemblBacteria" id="ABF12333">
        <property type="protein sequence ID" value="ABF12333"/>
        <property type="gene ID" value="Rmet_5474"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5474"/>
    <dbReference type="eggNOG" id="COG3181">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045683_0_0_4"/>
    <dbReference type="OMA" id="DKQRSAY"/>
    <dbReference type="OrthoDB" id="447734at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042597">
        <property type="term" value="C:periplasmic space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005064">
        <property type="entry name" value="BUG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042100">
        <property type="entry name" value="Bug_dom1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42928">
        <property type="entry name" value="PTHR42928"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03401">
        <property type="entry name" value="TctC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017082">
        <property type="entry name" value="YflP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-0675">Receptor</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004192992" evidence="3">
        <location>
            <begin position="25"/>
            <end position="327"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF12333.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="327" mass="35081" checksum="3930F8E5D7F94283" modified="2006-05-30" version="1">MKKRKLLLAAAGLFLGAAVPAANAAGAWPTAPITIVVPFAAGQTGDIIARLLGKELAERLKQPFVVDNKGGGGGRIGTAFVAKAKPDGYTLLMTSTGPYAIAPALYPRTMQYDPVRDFTGIAETATTPQIIAVSPKSGINTFADLVKQAKKSDMSYGSAGNGSTQHLTMELLKKELNFPLVHVPFKGSSESKTQVISGMIPVTSDSLPAIYTNIKSGQMKAIAVVDKQRSAYLPDVPTLAESGFPSLSTVAFFGLVAPKNTPKEVVNLLNKNLTEIYRTPDFQQKMKELAMTPPAEKTAEQFTAYLTEEVARWKKIVQDSNVKVEEY</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LC32</accession>
    <name>Q1LC32_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Alpha-methylacyl-CoA racemase</fullName>
            <ecNumber evidence="1">5.1.99.4</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_5435</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EC" id="5.1.99.4" evidence="1"/>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12294.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519841.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5435"/>
    <dbReference type="EnsemblBacteria" id="ABF12294">
        <property type="protein sequence ID" value="ABF12294"/>
        <property type="gene ID" value="Rmet_5435"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5435"/>
    <dbReference type="eggNOG" id="COG1804">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033975_5_0_4"/>
    <dbReference type="OMA" id="DCAAVNP"/>
    <dbReference type="OrthoDB" id="969868at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008111">
        <property type="term" value="F:alpha-methylacyl-CoA racemase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1540.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10540">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003673">
        <property type="entry name" value="CoA-Trfase_fam_III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044855">
        <property type="entry name" value="CoA-Trfase_III_dom3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023606">
        <property type="entry name" value="CoA-Trfase_III_dom_1_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02515">
        <property type="entry name" value="CoA_transf_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89796">
        <property type="entry name" value="SSF89796"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0413">Isomerase</keyword>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF12294.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="370" mass="39389" checksum="A077CFC9C96E08BB" modified="2006-05-30" version="1">MAGSLEGLNVVEFAGLGPAPFCCMLLADHGADVLRITRPTAPGVEAKVNPADVLARGRRTIPLDLREASGRDAALELIARADVVVEGFRPGVMERLGLGPDVCLARNPRLVYGRMTGWGQYGPLAQAAGHDINYIAISGALHAIGRAGEAPVPPLNLVGDFGGGGMMLAFGLMAALYEARQSGRGQVIDAAMTDGSALLSAMQYGMKAGGRWNNQRGENLLDGGAPFYDTYACADGKYVAVGAIEPQFYAALRERCGIEDPLFDGQMETARWPLLKLRLADIFRTRTRAEWCALLEGSDACFAPVLDWDEAPEHPHNREREAFVTLQGVVQPAAAPRFSRTPAAAPQSERRVSLDAALAHWGALQPASAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="96" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LC42</accession>
    <name>Q1LC42_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Putative short-chain specific (Butyryl-CoA dehydrogenase)</fullName>
            <ecNumber evidence="8">1.3.8.1</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Rmet_5425</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="9">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="9">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the acyl-CoA dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.3.8.1" evidence="8"/>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12284.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519833.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5425"/>
    <dbReference type="EnsemblBacteria" id="ABF12284">
        <property type="protein sequence ID" value="ABF12284"/>
        <property type="gene ID" value="Rmet_5425"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5425"/>
    <dbReference type="eggNOG" id="COG1960">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018204_0_2_4"/>
    <dbReference type="OMA" id="ICLEDAW"/>
    <dbReference type="OrthoDB" id="760677at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004085">
        <property type="term" value="F:butyryl-CoA dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.540.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.110.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006091">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046373">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036250">
        <property type="entry name" value="AcylCo_DH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009075">
        <property type="entry name" value="AcylCo_DH/oxidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013786">
        <property type="entry name" value="AcylCoA_DH/ox_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037069">
        <property type="entry name" value="AcylCoA_DH/ox_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009100">
        <property type="entry name" value="AcylCoA_DH/oxidase_NM_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00441">
        <property type="entry name" value="Acyl-CoA_dh_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02770">
        <property type="entry name" value="Acyl-CoA_dh_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02771">
        <property type="entry name" value="Acyl-CoA_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47203">
        <property type="entry name" value="SSF47203"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56645">
        <property type="entry name" value="SSF56645"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0274">FAD</keyword>
    <keyword evidence="3" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-0614">Plasmid</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Acyl-CoA_dh_N" evidence="7">
        <location>
            <begin position="6"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA_dh_M" evidence="6">
        <location>
            <begin position="122"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA_dh_1" evidence="5">
        <location>
            <begin position="226"/>
            <end position="372"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF02770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF02771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ABF12284.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="384" mass="41726" checksum="531BF5FB288F9450" modified="2006-05-30" version="1">MDFKLSEEHQAFADSVARFAQDKLKDGALARAHSPHYPWDTAALLAEQGLLGIAFSEEDGGQGGTLMHAVLAIEQIALVCPRSADIVQAGNFGPIRTFVEYASPEQKARYLPDLLAGRKVVSLGMSEPDAGSAVTELKTSARLDGDSYVINGSKVFSTHSPDAELFLIYVRFGPGVGGIGSVLVERGTPGFQVGEPSSFMNGEQWSQLYFDDCRIPVENVLLGPGGFKKQISGFNVERLGNSSRALALGRHAFNVAREHAMTRKQFGRELCEFQGIQWKFADMAMKLEQAQMMLYRAALEGEHGLPSAQSTAMAKLACNMAGWEVSNEALQVMGGMGFSQESLVEYCVRRTRGWMIAGGSIEILKNRVAEGVFGRTFSQRPTNG</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="60" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LC63</accession>
    <name>Q1LC63_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_5404</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12263.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519811.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5404"/>
    <dbReference type="EnsemblBacteria" id="ABF12263">
        <property type="protein sequence ID" value="ABF12263"/>
        <property type="gene ID" value="Rmet_5404"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5404"/>
    <dbReference type="eggNOG" id="ENOG502ZZ61">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1529777_0_0_4"/>
    <dbReference type="OMA" id="SEINWPR"/>
    <dbReference type="OrthoDB" id="1727434at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="48"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="82"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="128"/>
            <end position="149"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF12263.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="177" mass="20144" checksum="0AF8ADCA87D24FC7" modified="2006-05-30" version="1">MSKLIGTIKHEFMEMLPPTIYFFVILHIVAIIRALMVKGTGIELETTASVALAALILGKAVLLADMLPFINRFPEKPLIWNVGWKTVMYSLVALFIHYLERLYDFWKEAPGFAAANKLLVSEINWPRFWAIQILLLTLVLMYCTLAEMARAIGREKFKRAFFGPLPDNLANRSVNEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="64" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LCI6</accession>
    <name>Q1LCI6_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_5281</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12140.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519686.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF12140">
        <property type="protein sequence ID" value="ABF12140"/>
        <property type="gene ID" value="Rmet_5281"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5281"/>
    <dbReference type="eggNOG" id="ENOG5032B7Z">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_136209_0_0_4"/>
    <dbReference type="OMA" id="MAHAQDK"/>
    <dbReference type="OrthoDB" id="1855765at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193062" evidence="2">
        <location>
            <begin position="25"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="20"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="32"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="57"/>
            <end position="74"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF12140.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="113" mass="12114" checksum="B12705EED64845F9" modified="2006-05-30" version="1">MRKILLTSLIVATAAVAAAPMAHAQDKKQFDPYTQGAKSDQYNPYTDGARTGDKFDPYTQGANTSAQSNLVPDQKADPYTEGAKAGKYDPYTDGARTGKFDPFVDGAKSDSDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LCJ9</accession>
    <name>Q1LCJ9_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Oxidative demethylase of N1-methyladenine or N3-methylcytosine DNA lesions</fullName>
            <ecNumber evidence="7">1.14.11.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">alkB2</name>
        <name evidence="7" type="ordered locus">Rmet_5268</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="8">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="8">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
    </comment>
    <dbReference type="EC" id="1.14.11.-" evidence="7"/>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12127.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5268"/>
    <dbReference type="EnsemblBacteria" id="ABF12127">
        <property type="protein sequence ID" value="ABF12127"/>
        <property type="gene ID" value="Rmet_5268"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5268"/>
    <dbReference type="eggNOG" id="COG3145">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039677_1_1_4"/>
    <dbReference type="OMA" id="NCGPLGW"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051213">
        <property type="term" value="F:dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.590">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004574">
        <property type="entry name" value="Alkb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027450">
        <property type="entry name" value="AlkB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037151">
        <property type="entry name" value="AlkB-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005123">
        <property type="entry name" value="Oxoglu/Fe-dep_dioxygenase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16557">
        <property type="entry name" value="PTHR16557"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13532">
        <property type="entry name" value="2OG-FeII_Oxy_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51471">
        <property type="entry name" value="FE2OG_OXY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="2" id="KW-0227">DNA damage</keyword>
    <keyword evidence="5" id="KW-0234">DNA repair</keyword>
    <keyword evidence="7" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="4 7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-0614">Plasmid</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Fe2OG dioxygenase" evidence="6">
        <location>
            <begin position="123"/>
            <end position="223"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABF12127.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="223" mass="23988" checksum="468C524D5F8CDBE9" modified="2006-05-30" version="1">MPAMTFDLFDALPADSNAPPAVETLAPGAVVLHGFARDAGPELLAAVQTISAEAPFRHLITPGGLRMSVAMTNCGERGWVSDRTGYRYDAVDPESGRPWPAMPAVFRSLAEHAAEAAGYPGFAPDACLINRYLPGTRLSLHQDRDELDLRAPIVSVSMGLPAVFLWGGLRRADRPGRFRLAHGDVVVWGGPSRLVFHGIAPLADGDHALTGSERINLTFRKTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LCK4</accession>
    <name>Q1LCK4_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Flagellar assembly protein FliH</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">fliH</name>
        <name evidence="10" type="ordered locus">Rmet_5263</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="11">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="11">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Needed for flagellar regrowth and assembly.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the FliH family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12122.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519668.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5263"/>
    <dbReference type="EnsemblBacteria" id="ABF12122">
        <property type="protein sequence ID" value="ABF12122"/>
        <property type="gene ID" value="Rmet_5263"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5263"/>
    <dbReference type="eggNOG" id="COG1317">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_062625_4_1_4"/>
    <dbReference type="OMA" id="WALPSFD"/>
    <dbReference type="OrthoDB" id="1285795at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009288">
        <property type="term" value="C:bacterial-type flagellum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003774">
        <property type="term" value="F:cytoskeletal motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071973">
        <property type="term" value="P:bacterial-type flagellum-dependent cell motility"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000563">
        <property type="entry name" value="Flag_FliH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018035">
        <property type="entry name" value="Flagellar_FliH/T3SS_HrpE"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02108">
        <property type="entry name" value="FliH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01003">
        <property type="entry name" value="FLGFLIH"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1006">Bacterial flagellum protein export</keyword>
    <keyword evidence="10" id="KW-0966">Cell projection</keyword>
    <keyword evidence="10" id="KW-0969">Cilium</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0282">Flagellum</keyword>
    <keyword evidence="10" id="KW-0614">Plasmid</keyword>
    <keyword evidence="7" id="KW-0653">Protein transport</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="domain" description="FliH" evidence="9">
        <location>
            <begin position="137"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="27"/>
            <end position="41"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006602"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00016507"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF02108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ABF12122.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="278" mass="29705" checksum="18AD9BFCCD826AD2" modified="2006-05-30" version="1">MPTFDRGFSAGRGPGHGDPGFFGAPSTGTTGGLTNLHESDDGFQGFQPPARPRSRGESWQRWQMESLDPRDVLRKALAAQLPAEPPPPPAIDFEALDAMRDGARKEGYAQGYNQGQTQGYGDGHREGYARGLEAARNEAARMQQLAANFRDALGRVDNDISNALIGLALDVARQLVRKTVELDPAALLPAARELLTAEPPLSGAPCLLLNPEDIALVETHLSGELEAAGWTLRADPSIARGGCIASAASGEMDASLSTRWQRVVKALGRNDPWEPPHA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LDD3</accession>
    <name>Q1LDD3_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Putative permease MFS (Tn6048)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">mmfC1</name>
        <name evidence="2" type="ordered locus">Rmet_3001</name>
        <name evidence="3" type="ordered locus">Rmet_4036</name>
        <name evidence="4" type="ordered locus">Rmet_4981</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid</name>
    </geneLocation>
    <geneLocation type="plasmid" evidence="5">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="submission" date="2006-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of the chromosome of Ralstonia metallidurans CH34.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Copeland A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Barry K."/>
                <person name="Detter J.C."/>
                <person name="Glavina del Rio T."/>
                <person name="Hammon N."/>
                <person name="Israni S."/>
                <person name="Dalin E."/>
                <person name="Tice H."/>
                <person name="Martinez M."/>
                <person name="Goltsman E."/>
                <person name="Pitluck S."/>
                <person name="Schmutz J."/>
                <person name="Larimer F."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Kim E."/>
                <person name="Mergeay M."/>
                <person name="Benotmane M.A."/>
                <person name="Vallaeys T."/>
                <person name="Michaux A."/>
                <person name="Monchy S."/>
                <person name="Dunn J."/>
                <person name="McCorkle S."/>
                <person name="Taghavi S."/>
                <person name="van der Lelie D."/>
                <person name="Richardson P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">CH34</strain>
            <plasmid evidence="4">megaplasmid</plasmid>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="5">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2010-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Benotmane R."/>
                <person name="Lapidus A."/>
                <person name="McCorkle S."/>
                <person name="Monchy S."/>
                <person name="Taghavi S."/>
                <person name="van der Lelie N."/>
                <person name="Dunn J."/>
                <person name="Leys N."/>
                <person name="Mergeay M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">CH34</strain>
            <plasmid evidence="4">megaplasmid</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09874.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10904.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11843.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_005797951.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3001"/>
    <dbReference type="EnsemblBacteria" id="ABF09874">
        <property type="protein sequence ID" value="ABF09874"/>
        <property type="gene ID" value="Rmet_3001"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF10904">
        <property type="protein sequence ID" value="ABF10904"/>
        <property type="gene ID" value="Rmet_4036"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF11843">
        <property type="protein sequence ID" value="ABF11843"/>
        <property type="gene ID" value="Rmet_4981"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3001"/>
    <dbReference type="KEGG" id="rme:Rmet_4036"/>
    <dbReference type="KEGG" id="rme:Rmet_4981"/>
    <dbReference type="eggNOG" id="COG4705">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_070268_0_0_4"/>
    <dbReference type="OMA" id="RREIFYW"/>
    <dbReference type="OrthoDB" id="1466669at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007136">
        <property type="entry name" value="DUF347"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03988">
        <property type="entry name" value="DUF347"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0614">Plasmid</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="77"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="102"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="141"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="164"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="194"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="227"/>
            <end position="246"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF09874.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF10904.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF11843.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="260" mass="28101" checksum="329016CC82466906" modified="2006-05-30" version="1">MNKLPTSSTTGWLNKVPEVALSFWIIKIMSTTVGETGADFLAVNAGWGQGVTRTVMAALLAAALFMQLRTRRYTPWIYWLTVVLVSVVGTQITDLLTDGLGVSLYISTSAFAVALAAIFFVWYRIERTLSIHDIVTRSRELFYWAAILCTFALGTAAGDLATEALGLGFTWGAVAFGVLIGITYTAWRMGGNAVLTFWIAYILTRPFGAALGDLLTQAKTYGGLGMGAMWTSALFLTVIVMLVAVAQIGMNGRRSAQLVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LDH6</accession>
    <name>Q1LDH6_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Ubiquinone biosynthesis accessory factor UbiT</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">ubiT</name>
        <name evidence="3" type="ordered locus">Rmet_4938</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Required for O(2)-independent ubiquinone (coenzyme Q) biosynthesis. Likely functions as an accessory factor.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; ubiquinone biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the UbiT family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11800.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519360.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4938"/>
    <dbReference type="EnsemblBacteria" id="ABF11800">
        <property type="protein sequence ID" value="ABF11800"/>
        <property type="gene ID" value="Rmet_4938"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4938"/>
    <dbReference type="eggNOG" id="COG3154">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_111894_0_0_4"/>
    <dbReference type="OMA" id="AVPFRCE"/>
    <dbReference type="OrthoDB" id="1872111at2"/>
    <dbReference type="UniPathway" id="UPA00232"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006744">
        <property type="term" value="P:ubiquinone biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1050.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02231">
        <property type="entry name" value="UbiT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003033">
        <property type="entry name" value="SCP2_sterol-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036527">
        <property type="entry name" value="SCP2_sterol-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016830">
        <property type="entry name" value="UbiT"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02036">
        <property type="entry name" value="SCP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55718">
        <property type="entry name" value="SSF55718"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0831">Ubiquinone biosynthesis</keyword>
    <feature type="domain" description="SCP2" evidence="2">
        <location>
            <begin position="41"/>
            <end position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF11800.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="151" mass="16584" checksum="58B8CB5389F78694" modified="2006-05-30" version="1">MTMLTGLMSRVHRHMPAPARALPFVLAVEAMRRSGWLEPPAALNGHTFRLTVEDIGLEVRFRCVDGRFAPGPFDGSASDLTLRAKVADYLRLISGDADTDTLFFQRRLSISGDTELGLEVKYWLDAAPRPTWVGPFAARLSVLFPAPATAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LDL4</accession>
    <name>Q1LDL4_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">HTH cro/C1-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_4900</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11762.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4900"/>
    <dbReference type="EnsemblBacteria" id="ABF11762">
        <property type="protein sequence ID" value="ABF11762"/>
        <property type="gene ID" value="Rmet_4900"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4900"/>
    <dbReference type="eggNOG" id="COG3620">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2276247_0_0_4"/>
    <dbReference type="OMA" id="LEMMLVP"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00093">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001387">
        <property type="entry name" value="Cro/C1-type_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010982">
        <property type="entry name" value="Lambda_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="SMART" id="SM00530">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47413">
        <property type="entry name" value="SSF47413"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50943">
        <property type="entry name" value="HTH_CROC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH cro/C1-type" evidence="2">
        <location>
            <begin position="13"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="15"/>
            <end position="44"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF11762.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="105" mass="11235" checksum="8A2367166ADCD527" modified="2006-05-30" version="1">MSSIMSTALQSELESARRAAGVSQQELASRAGLSRMTVQRTESHEIDPRLSTVIEMARALGMDVMLVPTVLRPDLENFVRAGGKYLAQPPGGDAPPSVVDTLIAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LDR5</accession>
    <name>Q1LDR5_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative permease of the drug/metabolite transporter (DMT) superfamily</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_4849</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11711.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519274.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4849"/>
    <dbReference type="EnsemblBacteria" id="ABF11711">
        <property type="protein sequence ID" value="ABF11711"/>
        <property type="gene ID" value="Rmet_4849"/>
    </dbReference>
    <dbReference type="GeneID" id="60823897"/>
    <dbReference type="KEGG" id="rme:Rmet_4849"/>
    <dbReference type="eggNOG" id="COG0697">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033863_4_4_4"/>
    <dbReference type="OMA" id="WANEYVS"/>
    <dbReference type="OrthoDB" id="1515911at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000620">
        <property type="entry name" value="EamA_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00892">
        <property type="entry name" value="EamA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="38"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="70"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="99"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="127"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="156"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="188"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="222"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="253"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="278"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="domain" description="EamA" evidence="2">
        <location>
            <begin position="13"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="domain" description="EamA" evidence="2">
        <location>
            <begin position="158"/>
            <end position="293"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF11711.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="297" mass="32693" checksum="28CEECB843DBBAD5" modified="2006-05-30" version="1">MKTTRIDATTLFLLTFPPLAWAGNAIVGRLAAGTVPPITLNLVRWIIAALLLAPYAWRGVMTHRAALRQHLAMLATMGLLSIASYNAFQYLALTTSTPINVTLIGASTPLFLLLIGACFFHERFRPWQVAGALLCLLGVSFVLMRGEPERLAHLEFVPGDLYMLAATITWSFYTWLLRKHRPDLPLPVLLLAQIVAGVIASIPVAAWELGRLTEPLQWSGKVAWILLYVGTVPSLLAYFVWDRAISRAGAQLPVFFITLTPLFAAVLSTVLLGESPHWYHGMGLVCIGAGIWLAQRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="63" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LEG9</accession>
    <name>Q1LEG9_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Regulator, CzcI-like: Cobalt-zinc-cadmium resistance protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">czcI2</name>
        <name evidence="3" type="ordered locus">Rmet_4595</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11457.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519038.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4595"/>
    <dbReference type="EnsemblBacteria" id="ABF11457">
        <property type="protein sequence ID" value="ABF11457"/>
        <property type="gene ID" value="Rmet_4595"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4595"/>
    <dbReference type="eggNOG" id="ENOG5032Y09">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_151310_0_0_4"/>
    <dbReference type="OMA" id="LQFAWAG"/>
    <dbReference type="OrthoDB" id="2017919at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193125" evidence="2">
        <location>
            <begin position="21"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="43"/>
            <end position="68"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF11457.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="125" mass="13779" checksum="79D330DA1B9C6BB9" modified="2006-05-30" version="1">MRRLLLVFLAILLPLQFAWAGAAAYCAHEKATSVDARQWHFGHHTHEHKAADGDRQAQDAKDSKSAKLPDGDCNACHFPGSHGLFSELAMTGDYPFVTVHYQPRASAFGSIPSPVPDRPQWSRLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="105" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LER2</accession>
    <name>Q1LER2_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">RNA polymerase sigma J factor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">rpoJ</name>
        <name evidence="4" type="ordered locus">Rmet_4499</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="5">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="5">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the sigma-70 factor family. ECF subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11364.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008643876.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4499"/>
    <dbReference type="EnsemblBacteria" id="ABF11364">
        <property type="protein sequence ID" value="ABF11364"/>
        <property type="gene ID" value="Rmet_4499"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4499"/>
    <dbReference type="eggNOG" id="COG1595">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047691_12_1_4"/>
    <dbReference type="OMA" id="CYVLRYE"/>
    <dbReference type="OrthoDB" id="1914729at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016987">
        <property type="term" value="F:sigma factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006352">
        <property type="term" value="P:DNA-templated transcription, initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050896">
        <property type="term" value="P:response to stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039425">
        <property type="entry name" value="RNA_pol_sigma-70-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014284">
        <property type="entry name" value="RNA_pol_sigma-70_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007627">
        <property type="entry name" value="RNA_pol_sigma70_r2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013249">
        <property type="entry name" value="RNA_pol_sigma70_r4_t2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013325">
        <property type="entry name" value="RNA_pol_sigma_r2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013324">
        <property type="entry name" value="RNA_pol_sigma_r3/r4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43133">
        <property type="entry name" value="PTHR43133"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04542">
        <property type="entry name" value="Sigma70_r2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08281">
        <property type="entry name" value="Sigma70_r4_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88659">
        <property type="entry name" value="SSF88659"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88946">
        <property type="entry name" value="SSF88946"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02937">
        <property type="entry name" value="sigma70-ECF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0614">Plasmid</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Sigma70_r2" evidence="2">
        <location>
            <begin position="12"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="domain" description="Sigma70_r4_2" evidence="3">
        <location>
            <begin position="108"/>
            <end position="159"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF04542"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF08281"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF11364.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="166" mass="19007" checksum="D77B02337FB9942E" modified="2006-05-30" version="1">MDPGTRSQVDVLYSRHHAWLQGWLLRKIGNACDAADIAQDVFVRVLHRKVVEIREPRTYLGTIANGLVIDFWRRRELEQAWLETLAQLPEAHTPSPESRLHFLQTLIEIDQMLDTLKPNARTAFLLAQLDGLTCPQIAERIGMSLATVERYISQALRRCYALQFGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="109" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LEU6</accession>
    <name>Q1LEU6_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Two component heavy metal response transcriptional regulator, winged helix family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">czcR2</name>
        <name evidence="5" type="ordered locus">Rmet_4465</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="6">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="6">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11330.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518932.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4465"/>
    <dbReference type="EnsemblBacteria" id="ABF11330">
        <property type="protein sequence ID" value="ABF11330"/>
        <property type="gene ID" value="Rmet_4465"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4465"/>
    <dbReference type="eggNOG" id="COG0745">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_30_1_4"/>
    <dbReference type="OMA" id="PNDRSID"/>
    <dbReference type="OrthoDB" id="1020672at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00383">
        <property type="entry name" value="trans_reg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001867">
        <property type="entry name" value="OmpR/PhoB-type_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006291">
        <property type="entry name" value="PcoR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039420">
        <property type="entry name" value="WalR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48111">
        <property type="entry name" value="PTHR48111"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00486">
        <property type="entry name" value="Trans_reg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00862">
        <property type="entry name" value="Trans_reg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01387">
        <property type="entry name" value="cztR_silR_copR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51755">
        <property type="entry name" value="OMPR_PHOB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="5" id="KW-0614">Plasmid</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Response regulatory" evidence="3">
        <location>
            <begin position="2"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="domain" description="OmpR/PhoB-type" evidence="4">
        <location>
            <begin position="123"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="OmpR/PhoB-type" evidence="2">
        <location>
            <begin position="123"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="1">
        <location>
            <position position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABF11330.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="206" mass="23459" checksum="236919A058B3AE84" modified="2006-05-30" version="1">MRILIVEDEPKAGDYLLKGLTESGFVADLARDGVDGLAHAREQHYDLIVLDVMLPGMDGWQVLRELRRDKDTPVLCLTARDELSDRLKGLELGADDYMVKPFAFAELVARIRTILRRGPLRESEFIEVADLQIDTIRRRVTRAGQKIDLTSKEFALLQLLARRRGEVLSRSLIASQVWDMNFDSNTNVVDVSIRRLRAKVDDPFPN</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="65" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LEV6</accession>
    <name>Q1LEV6_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_4455</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11320.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518924.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4455"/>
    <dbReference type="EnsemblBacteria" id="ABF11320">
        <property type="protein sequence ID" value="ABF11320"/>
        <property type="gene ID" value="Rmet_4455"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4455"/>
    <dbReference type="eggNOG" id="COG1878">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030671_0_0_4"/>
    <dbReference type="OMA" id="DVIAMPL"/>
    <dbReference type="OrthoDB" id="831439at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004061">
        <property type="term" value="F:arylformamidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019441">
        <property type="term" value="P:tryptophan catabolic process to kynurenine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.30.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007325">
        <property type="entry name" value="KFase/CYL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037175">
        <property type="entry name" value="KFase_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04199">
        <property type="entry name" value="Cyclase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102198">
        <property type="entry name" value="SSF102198"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF11320.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="320" mass="35222" checksum="2BBB1FF3887DE2BF" modified="2006-05-30" version="1">MESKNVPHVAELLRDAPKNWGKWGSDDEVGSLNYLTQAEVLRGVAAVRSGKTFTLQIQMGNPKGDPVWPGRSQARRLNVMDKGHYLCGKAPIFPGQGEYADDMMIMYLQGSTQYDALGHVWYGDQLWNGYDAKSTIGGMEKASVLPIAERGIVGRGVLIDMARHRGKAVLDPGETFTHLDLLAAAEMQGVRIEKHDIVIIRTGWIGSFYERDQAEFYKNFIEPGLTYSPELVEWFHKMEIPNLVTDTIANEVTVDPVSGVALPLHNALMRNLGVTLTEIAQLDPLATDCADDGQWSFLYTAAPLKVVNGSGAPVNPVVIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LF75</accession>
    <name>Q1LF75_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Fatty acid desaturase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Rmet_4336</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="5">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="5">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11201.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4336"/>
    <dbReference type="EnsemblBacteria" id="ABF11201">
        <property type="protein sequence ID" value="ABF11201"/>
        <property type="gene ID" value="Rmet_4336"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4336"/>
    <dbReference type="eggNOG" id="COG3239">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_053288_0_0_4"/>
    <dbReference type="OMA" id="WYMSLQH"/>
    <dbReference type="OrthoDB" id="1032884at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005804">
        <property type="entry name" value="FA_desaturase_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00487">
        <property type="entry name" value="FA_desaturase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0614">Plasmid</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="54"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="domain" description="FA_desaturase" evidence="3">
        <location>
            <begin position="82"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="27"/>
            <end position="45"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF11201.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="343" mass="39634" checksum="7E2BFA34E11FD089" modified="2006-05-30" version="1">MSLVTPVSTGPATKTIESAPTGKAKVPATDYSTSPAHSTRLPTPKRSSHAWDRYELPTIALAAVLYAAWAALVWWHASIPGPLLFLAGGYVAQLHFSLQHESIHAMRHLPIWLRTAVVWPPINLWLPYPFYNKGHSTHHINVHLTHPQKDTETAYHSPTQWQAMSVTWRAIYRANQILPVRLICGPFLRLVRLARNEYRCLIAGDFSRLKIWAIHALSATPLLYFALALAGMRWWEYLLYFVYPGMMLGQLRTFTEHRWGERPVERTAIVESNSIGGLLYLYNNLHYVHHLFPTLPWYHIPRYFRAHRAAVLHGNGGFYYRGYAEIARRFWRTSVFDPVHPRW</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LFP3</accession>
    <name>Q1LFP3_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Putative transporter, exporter RND family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">hpnN</name>
        <name evidence="9" type="ordered locus">Rmet_4166</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="10">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="10">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11033.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518655.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4166"/>
    <dbReference type="EnsemblBacteria" id="ABF11033">
        <property type="protein sequence ID" value="ABF11033"/>
        <property type="gene ID" value="Rmet_4166"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4166"/>
    <dbReference type="eggNOG" id="COG4258">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009099_0_0_4"/>
    <dbReference type="OMA" id="AFKIYYV"/>
    <dbReference type="OrthoDB" id="149255at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017841">
        <property type="entry name" value="Hopanoid_biosynth_HpnN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004869">
        <property type="entry name" value="MMPL_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03176">
        <property type="entry name" value="MMPL"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03480">
        <property type="entry name" value="HpnN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0614">Plasmid</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="12"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="278"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="304"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="331"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="375"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="408"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="458"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="720"/>
            <end position="741"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="753"/>
            <end position="774"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="780"/>
            <end position="800"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="812"/>
            <end position="832"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="844"/>
            <end position="864"/>
        </location>
    </feature>
    <feature type="domain" description="MMPL" evidence="8">
        <location>
            <begin position="48"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="domain" description="MMPL" evidence="8">
        <location>
            <begin position="655"/>
            <end position="865"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="869"/>
            <end position="908"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="886"/>
            <end position="901"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF03176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ABF11033.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="908" mass="95866" checksum="4EECEBBB0FF3C1B3" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LFP6</accession>
    <name>Q1LFP6_CUPMC</name>
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        <recommendedName>
            <fullName evidence="2">LysM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_4163</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
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    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
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    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4163"/>
    <dbReference type="EnsemblBacteria" id="ABF11030">
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        <property type="gene ID" value="Rmet_4163"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4163"/>
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        <property type="taxonomic scope" value="Bacteria"/>
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    <dbReference type="OMA" id="RYMASEQ"/>
    <dbReference type="OrthoDB" id="1833920at2"/>
    <dbReference type="Proteomes" id="UP000002429">
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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            <begin position="1"/>
            <end position="27"/>
        </location>
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        <location>
            <begin position="28"/>
            <end position="556"/>
        </location>
    </feature>
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        <location>
            <begin position="37"/>
            <end position="84"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF11030.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="556" mass="57874" checksum="609D8BA72482270E" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LFX5</accession>
    <name>Q1LFX5_CUPMC</name>
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        <submittedName>
            <fullName evidence="3">Alkaline phosphatase</fullName>
            <ecNumber evidence="3">3.1.3.1</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">phoA1</name>
        <name evidence="3" type="ordered locus">Rmet_4084</name>
    </gene>
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        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
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            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
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    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the alkaline phosphatase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.1" evidence="3"/>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10951.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4084"/>
    <dbReference type="EnsemblBacteria" id="ABF10951">
        <property type="protein sequence ID" value="ABF10951"/>
        <property type="gene ID" value="Rmet_4084"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4084"/>
    <dbReference type="eggNOG" id="COG1785">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008539_4_1_4"/>
    <dbReference type="OMA" id="CANMQVA"/>
    <dbReference type="OrthoDB" id="585847at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004035">
        <property type="term" value="F:alkaline phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd16012">
        <property type="entry name" value="ALP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.720.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001952">
        <property type="entry name" value="Alkaline_phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017850">
        <property type="entry name" value="Alkaline_phosphatase_core_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11596">
        <property type="entry name" value="PTHR11596"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00245">
        <property type="entry name" value="Alk_phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00113">
        <property type="entry name" value="ALKPHPHTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00098">
        <property type="entry name" value="alkPPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53649">
        <property type="entry name" value="SSF53649"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193101" evidence="2">
        <location>
            <begin position="18"/>
            <end position="486"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU003946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF10951.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="486" mass="50449" checksum="B01EDEFEADF8A74D" modified="2006-05-30" version="1">MRRISVLAAAAMSVALAACGGDDNNGNNAGTTPPIEQPQGGTTPTKNVVFFLGDGMGMTTLTAARIYKVGEEGDLTIDTMPESGFVRTYSNDAQVTDSAPSMAAYMTGVKMNNEVISMTPETSAVDANGKAYVSGADSTCPATGNGKSVPTLLEQMKAAGYGTGVVTTTRITHATPAATYAHICHRDGENAIAAQLVPKGAGFNGALTDGVDVIFGGGRQHFLATTAGGKRTDGRDLVGEFKTAGYSYAASKSDFDKITASSAKVVGLFNASHMDYDLDRDPAKEPSLAEMTGKAIDVLAAKKKGFFLMVEGGRIDHALHETTARKALQDTAAFDDAIKAAIDKLNVIDPGLKNTLIVVTADHDHTLVLNGYAKRTGKTSDANPGVLGLVKDYVTGSAKLDSTGNPFSIIGFGNGENRPATRTALTDVQVFDKTYHQEAVIPMPAGGETHGGTDVFIGAMGNGATSFTGVMDNIEVFGLIKKAIGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="58" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LGB3</accession>
    <name>Q1LGB3_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_3945</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10813.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3945"/>
    <dbReference type="EnsemblBacteria" id="ABF10813">
        <property type="protein sequence ID" value="ABF10813"/>
        <property type="gene ID" value="Rmet_3945"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3945"/>
    <dbReference type="HOGENOM" id="CLU_140889_0_0_4"/>
    <dbReference type="OMA" id="ASQRQHC"/>
    <dbReference type="OrthoDB" id="1940591at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="59"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="59"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="82"/>
            <end position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF10813.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="105" mass="11329" checksum="4E26CC94335659BB" modified="2006-05-30" version="1">MSVAQVHAAPPQDPGTPIVAPLPPRMLDPATSTVEDTGGTLSASQVERCRQLLDQINALPSGPQWGQGKSSVTTADGRTYSTLERAPDRKRLEEAYRQECAQSRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="98" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LGG9</accession>
    <name>Q1LGG9_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Putative transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Rmet_3889</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="6">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="6">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10757.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518404.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3889"/>
    <dbReference type="EnsemblBacteria" id="ABF10757">
        <property type="protein sequence ID" value="ABF10757"/>
        <property type="gene ID" value="Rmet_3889"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3889"/>
    <dbReference type="eggNOG" id="COG1802">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017584_5_1_4"/>
    <dbReference type="OMA" id="VLLHTWE"/>
    <dbReference type="OrthoDB" id="1911275at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07377">
        <property type="entry name" value="WHTH_GntR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.530">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011711">
        <property type="entry name" value="GntR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008920">
        <property type="entry name" value="TF_FadR/GntR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000524">
        <property type="entry name" value="Tscrpt_reg_HTH_GntR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07729">
        <property type="entry name" value="FCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00392">
        <property type="entry name" value="GntR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00895">
        <property type="entry name" value="FCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00345">
        <property type="entry name" value="HTH_GNTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48008">
        <property type="entry name" value="SSF48008"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50949">
        <property type="entry name" value="HTH_GNTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0614">Plasmid</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH gntR-type" evidence="4">
        <location>
            <begin position="16"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABF10757.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="240" mass="26627" checksum="FA27350CA2E6286B" modified="2006-05-30" version="1">MSVTQALPKTARLRTLGMSAEIAARLRTMIQEGELPPGVRIDEKAFCEAFDVSKTPLREALKVLVAEGLVLHRQYIGYRVAPLDLDELRATFETLHGLEAHAGELAAQRLSDAAVTKIERRHQAMIDAHDAGRRTDYFRINQEIHQLIIDGAGNAVLAGIYAMLMSKVHRARGAANADMLRWQESHTEHEEIIAALREPGRPRLADVLRRHSENTAKEVLRVVEQTLAEPAARAAGGARR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LGW9</accession>
    <name>Q1LGW9_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 6">Flagellar basal-body rod protein FlgC</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">flgC</name>
        <name evidence="9" type="ordered locus">Rmet_3735</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="10">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="10">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="5 6">The basal body constitutes a major portion of the flagellar organelle and consists of four rings (L,P,S, and M) mounted on a central rod. The rod consists of about 26 subunits of FlgG in the distal portion, and FlgB, FlgC and FlgF are thought to build up the proximal portion of the rod with about 6 subunits each.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Bacterial flagellum basal body</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the flagella basal body rod proteins family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10607.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3735"/>
    <dbReference type="EnsemblBacteria" id="ABF10607">
        <property type="protein sequence ID" value="ABF10607"/>
        <property type="gene ID" value="Rmet_3735"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3735"/>
    <dbReference type="eggNOG" id="COG1558">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_123272_1_0_4"/>
    <dbReference type="OMA" id="YVAYPNI"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030694">
        <property type="term" value="C:bacterial-type flagellum basal body, rod"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071973">
        <property type="term" value="P:bacterial-type flagellum-dependent cell motility"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001444">
        <property type="entry name" value="Flag_bb_rod_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019776">
        <property type="entry name" value="Flagellar_basal_body_rod_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010930">
        <property type="entry name" value="Flg_bb/hook_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006299">
        <property type="entry name" value="FlgC"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00460">
        <property type="entry name" value="Flg_bb_rod"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06429">
        <property type="entry name" value="Flg_bbr_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01395">
        <property type="entry name" value="FlgC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00588">
        <property type="entry name" value="FLAGELLA_BB_ROD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0975">Bacterial flagellum</keyword>
    <keyword evidence="9" id="KW-0966">Cell projection</keyword>
    <keyword evidence="9" id="KW-0969">Cilium</keyword>
    <keyword evidence="9" id="KW-0282">Flagellum</keyword>
    <keyword evidence="9" id="KW-0614">Plasmid</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Flg_bb_rod" evidence="7">
        <location>
            <begin position="7"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="domain" description="Flg_bbr_C" evidence="8">
        <location>
            <begin position="88"/>
            <end position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00017941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023143"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00025933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU362062"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF06429"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ABF10607.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="135" mass="14198" checksum="173A029A8D69DA32" modified="2006-05-30" version="1">MGTMSIFDVAGSAMAAQSQRMNVTASNLANADSAVAPNGQPYRAKQVIFEMAPTPGQSDVGGVQVAGVMEDTAPARMVHNPSHPLADANGYVTMPNVNPVEEMVNMISASRSYQANVEVLNTAKNMMLKTLTIGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="63" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LGZ0</accession>
    <name>Q1LGZ0_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_3714</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10586.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008641111.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3714"/>
    <dbReference type="DNASU" id="4040685"/>
    <dbReference type="EnsemblBacteria" id="ABF10586">
        <property type="protein sequence ID" value="ABF10586"/>
        <property type="gene ID" value="Rmet_3714"/>
    </dbReference>
    <dbReference type="GeneID" id="60826268"/>
    <dbReference type="KEGG" id="rme:Rmet_3714"/>
    <dbReference type="eggNOG" id="ENOG5033A4Z">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_176022_4_1_4"/>
    <dbReference type="OMA" id="GVWGWIG"/>
    <dbReference type="OrthoDB" id="2089632at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021309">
        <property type="entry name" value="DUF2892"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11127">
        <property type="entry name" value="DUF2892"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="31"/>
            <end position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF10586.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="66" mass="7133" checksum="93FE82CF468C24D7" modified="2006-05-30" version="1">MQKNAGTLDRLVRVAIGVVLIALTLTDQIGVWGWIGAVPLVTGLLGYCPLYRVFGFNTCPITRRGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LH09</accession>
    <name>Q1LH09_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 7">Protein phosphatase CheZ</fullName>
            <ecNumber evidence="7">3.1.3.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6 7">Chemotaxis protein CheZ</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="primary">cheZ</name>
        <name evidence="10" type="ordered locus">Rmet_3695</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="11">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="11">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Plays an important role in bacterial chemotaxis signal transduction pathway by accelerating the dephosphorylation of phosphorylated CheY (CheY-P).</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the CheZ family.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.-" evidence="7"/>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10567.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518216.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3695"/>
    <dbReference type="EnsemblBacteria" id="ABF10567">
        <property type="protein sequence ID" value="ABF10567"/>
        <property type="gene ID" value="Rmet_3695"/>
    </dbReference>
    <dbReference type="GeneID" id="60826250"/>
    <dbReference type="KEGG" id="rme:Rmet_3695"/>
    <dbReference type="eggNOG" id="COG3143">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_080718_1_0_4"/>
    <dbReference type="OMA" id="EGPQIHA"/>
    <dbReference type="OrthoDB" id="1206193at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009288">
        <property type="term" value="C:bacterial-type flagellum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004721">
        <property type="term" value="F:phosphoprotein phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097588">
        <property type="term" value="P:archaeal or bacterial-type flagellum-dependent cell motility"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006935">
        <property type="term" value="P:chemotaxis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050920">
        <property type="term" value="P:regulation of chemotaxis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007439">
        <property type="entry name" value="Chemotax_Pase_CheZ"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04344">
        <property type="entry name" value="CheZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002884">
        <property type="entry name" value="CheZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0145">Chemotaxis</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 7" id="KW-0283">Flagellar rotation</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-0614">Plasmid</keyword>
    <keyword evidence="7" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="176"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="site" description="Enhances dephosphorylation of CheY-P" evidence="8">
        <location>
            <position position="145"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00018484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022500"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022779"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00029599"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR002884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR002884-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ABF10567.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="211" mass="23612" checksum="E8E3F03D115F6717" modified="2006-05-30" version="1">MSTTPTFSNEAAEQIIHRIGNLTRMLRDNMRELGLDKEIERAAQAIPDARDRLSYIAAMTEQAAERSLTAVELAQPLQEELEAKATALDKRWGEWFEQPVELGDARALVLDTREFLTEVPEKTKATGSHLLEIMMAQDFQDLTGQVIKKMMDMIRTLEQELLQVLIDNVPADRRVEAPPSLINGPQINPEGKADVVSDQSQVDDLLASLGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LH41</accession>
    <name>Q1LH41_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 7">Nuclease SbcCD subunit D</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7 10" type="primary">sbcD</name>
        <name evidence="10" type="ordered locus">Rmet_3663</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="11">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="11">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">SbcCD cleaves DNA hairpin structures. These structures can inhibit DNA replication and are intermediates in certain DNA recombination reactions. The complex acts as a 3'-&gt;5' double strand exonuclease that can open hairpins. It also has a 5' single-strand endonuclease activity.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2 7">Heterodimer of SbcC and SbcD.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the SbcD family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10535.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518190.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3663"/>
    <dbReference type="EnsemblBacteria" id="ABF10535">
        <property type="protein sequence ID" value="ABF10535"/>
        <property type="gene ID" value="Rmet_3663"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3663"/>
    <dbReference type="eggNOG" id="COG0420">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038045_0_1_4"/>
    <dbReference type="OMA" id="TSGNHDS"/>
    <dbReference type="OrthoDB" id="1831911at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008408">
        <property type="term" value="F:3'-5' exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00840">
        <property type="entry name" value="MPP_Mre11_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP_ApaH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041796">
        <property type="entry name" value="Mre11_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004593">
        <property type="entry name" value="SbcD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026843">
        <property type="entry name" value="SbcD_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12320">
        <property type="entry name" value="SbcD_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="SSF56300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00619">
        <property type="entry name" value="sbcd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="7" id="KW-0235">DNA replication</keyword>
    <keyword evidence="7" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="6 7 10" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="5 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 7" id="KW-0540">Nuclease</keyword>
    <keyword evidence="10" id="KW-0614">Plasmid</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Metallophos" evidence="8">
        <location>
            <begin position="1"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="domain" description="SbcD_C" evidence="9">
        <location>
            <begin position="264"/>
            <end position="352"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013365"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU363069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF12320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ABF10535.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="381" mass="40759" checksum="0D0DA47A9D2607FF" modified="2006-05-30" version="1">MRFLHTADWHLGRLFHARSLIEDQSHVLDQFVALVRDLRPDAVLIAGDVYDRAVPPPEAVALLDDVLARIVVDAGVPVVMIAGNHDSAQRLGFGARLLAARGLHVAGRTGPETSCVTLHDAHGEVRIYALPYAEPAAVRDALGTDLPTHEAALNAQLDAVRATHPAGVRSVAVAHAFVVGGAVSESERPLSVGGSGAVAADVFHGFDFVALGHLHRPQSIGARIHYSGSLLKYSLSETEHIKTVSLIDVDAEGGVRIEAIPLRPLRELRVLTGTLAGLLEAGATDPHRDDYVHAVLTDAGALLDPMARLRQVYPNALAIERAVLARSGQAGEAGRKLRELDTASLFANFFREVADTDLDDDQRRALDDLLAVMQASERESA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="54" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LH97</accession>
    <name>Q1LH97_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_3607</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10479.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008650831.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3607"/>
    <dbReference type="EnsemblBacteria" id="ABF10479">
        <property type="protein sequence ID" value="ABF10479"/>
        <property type="gene ID" value="Rmet_3607"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3607"/>
    <dbReference type="HOGENOM" id="CLU_1414147_0_0_4"/>
    <dbReference type="OMA" id="CEVEARA"/>
    <dbReference type="OrthoDB" id="1569461at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024422">
        <property type="entry name" value="Protein_unknown_function_OB"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12869">
        <property type="entry name" value="tRNA_anti-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="7"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="36"/>
            <end position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF10479.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="188" mass="20040" checksum="5C9C236BC2A35E22" modified="2006-05-30" version="1">MLSRLQQSLIAVVAVVVLGLLWFVGNDKGDVVPVETGAAPSSQPASIPRGIPRNGTLDPISIDPERPADVLPQYQADGLFADYRDNAGEADDRYRGKYFIVEGVASGIRRDDGNNVFLEIRTSNPGELVRANLLRRQICGPAGRVCEVEARATMVRRGQKVAVECTGAGQGEGGVPLLNDCLIRGGAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="96" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LHC6</accession>
    <name>Q1LHC6_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Glyoxylate reductase</fullName>
            <ecNumber evidence="4">1.1.1.95</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">gyaR</name>
        <name evidence="4" type="ordered locus">Rmet_3578</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.95" evidence="4"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10450.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3578"/>
    <dbReference type="EnsemblBacteria" id="ABF10450">
        <property type="protein sequence ID" value="ABF10450"/>
        <property type="gene ID" value="Rmet_3578"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3578"/>
    <dbReference type="eggNOG" id="COG0111">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019796_1_3_4"/>
    <dbReference type="OMA" id="KHGAWQQ"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004617">
        <property type="term" value="F:phosphoglycerate dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006139">
        <property type="entry name" value="D-isomer_2_OHA_DH_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006140">
        <property type="entry name" value="D-isomer_DH_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00389">
        <property type="entry name" value="2-Hacid_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02826">
        <property type="entry name" value="2-Hacid_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="2-Hacid_dh" evidence="2">
        <location>
            <begin position="52"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="domain" description="2-Hacid_dh_C" evidence="3">
        <location>
            <begin position="140"/>
            <end position="325"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU003719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF02826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF10450.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="366" mass="40374" checksum="E9EBB61E3E7493E6" modified="2006-05-30" version="1">MLCQEIQQYCRLRTDSVAVRTEPEVKESPMKIAVLDDYQDAVRKLPCFALLEGHDVKVFNNTVKGVGQLAARLSDVEALVLIRERTRVTRQLLEKLPKLRIISQTGRAGNHVDIEACTERGVVVLEGVGSPVAPAELTWALIMAAQRRIPQYVASLKHGAWQQSGLKSTTMPPNFGLGQVLRGQTLGIWGYGKIGRLLAGYGRAFGMNVLVWGREASQQGAREAGLNVAESVEQLFADSDVLSLHLRLNDETRGIVKLADLQRMKPTSLFVNTSRAELIEENALVAALNRGRPGMAAIDVFESEPILQGHALLRMENCICTPHLGYVERESYELYFRAAFQNILDVLAGKHDSIANPKALTPTLAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LHM1</accession>
    <name>Q1LHM1_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">L-serine dehydratase</fullName>
            <ecNumber evidence="9">4.3.1.17</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">sdaA</name>
        <name evidence="12" type="ordered locus">Rmet_3483</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 9">
            <text>L-serine = NH4(+) + pyruvate</text>
            <dbReference type="Rhea" id="RHEA:19169"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
            <dbReference type="EC" id="4.3.1.17"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 9">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the iron-sulfur dependent L-serine dehydratase family.</text>
    </comment>
    <dbReference type="EC" id="4.3.1.17" evidence="9"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10355.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517912.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3483"/>
    <dbReference type="EnsemblBacteria" id="ABF10355">
        <property type="protein sequence ID" value="ABF10355"/>
        <property type="gene ID" value="Rmet_3483"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3483"/>
    <dbReference type="eggNOG" id="COG1760">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022305_0_1_4"/>
    <dbReference type="OMA" id="ELHRDTY"/>
    <dbReference type="OrthoDB" id="225730at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003941">
        <property type="term" value="F:L-serine ammonia-lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006094">
        <property type="term" value="P:gluconeogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029009">
        <property type="entry name" value="ASB_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004644">
        <property type="entry name" value="Fe-S_L-Ser_mono"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005130">
        <property type="entry name" value="Ser_deHydtase-like_asu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005131">
        <property type="entry name" value="Ser_deHydtase_bsu"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03313">
        <property type="entry name" value="SDH_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03315">
        <property type="entry name" value="SDH_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143548">
        <property type="entry name" value="SSF143548"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00720">
        <property type="entry name" value="sda_mono"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 9" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="9" id="KW-0312">Gluconeogenesis</keyword>
    <keyword evidence="6 9" id="KW-0408">Iron</keyword>
    <keyword evidence="7 9" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="8 9 12" id="KW-0456">Lyase</keyword>
    <keyword evidence="5 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SDH_beta" evidence="11">
        <location>
            <begin position="4"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="domain" description="SDH_alpha" evidence="10">
        <location>
            <begin position="188"/>
            <end position="452"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008636"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU366059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF03313"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF03315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ABF10355.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="458" mass="48760" checksum="2989D854FAA8CD99" modified="2006-05-30" version="1">MAVSVFDLFKVGIGPSSSHTVGPMRAALMFAHGLERDGLLPQVASVRVELYGSLGATGKGHGTDKGVILGLMGEAPDTVDPNTIDAKLAALRASRQLSLLGTHTVPFVEREHIAFYRREAMAEHPNGMKFHAYDTDGTSLREARYLSVGGGFIVTAGSPNTQLQAAERQLPHPFRSGRAMLDMAHASGKSIARLMLENELTWRSEQEVMDGLLHIWDVMQQCVARGCRTDGELPGPFKVRRRAPELYRNLTERAERTLSDPLSVMDWVNLYAIAVNEENAAGGRVVTAPTNGAAGIIPAVLHYYDRFIPGANPQGAVDFLLTAGAIGLLYKLNASISGAEVGCQGEVGVACSMAAGALAAVLGGTPEQVENAAEIGMEHNLGLTCDPVGGLVQIPCIERNAMAAVKAVNAARMALRGDGTHYVSLDSVIKTMRETGADMKTKYKETARGGLAVNIVEC</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-12-02" version="62" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LHY8</accession>
    <name>Q1LHY8_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_3366</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10238.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3366"/>
    <dbReference type="EnsemblBacteria" id="ABF10238">
        <property type="protein sequence ID" value="ABF10238"/>
        <property type="gene ID" value="Rmet_3366"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3366"/>
    <dbReference type="HOGENOM" id="CLU_2976091_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011335">
        <property type="entry name" value="Restrct_endonuc-II-like"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52980">
        <property type="entry name" value="SSF52980"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF10238.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="58" mass="6562" checksum="5CF6A166293ECB55" modified="2006-05-30" version="1">MHSLCSGRSSSCMDVFWHGHSCKEGTREPKSNQACWVPRIAANHEQDCRQISELMELG</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="72" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LHZ8</accession>
    <name>Q1LHZ8_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">ATP-binding region, ATPase-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_3356</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10228.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517795.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3356"/>
    <dbReference type="EnsemblBacteria" id="ABF10228">
        <property type="protein sequence ID" value="ABF10228"/>
        <property type="gene ID" value="Rmet_3356"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3356"/>
    <dbReference type="eggNOG" id="ENOG5033RKN">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_751717_0_0_4"/>
    <dbReference type="OMA" id="TACPNTH"/>
    <dbReference type="OrthoDB" id="1667523at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="SSF55874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF10228.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="367" mass="41036" checksum="2D8D9138B37E3DA6" modified="2006-05-30" version="1">MKEQIRQILSDGQPRKARELAVALSIETTSVNQFLYRNLDTFTKNADHRWTLASLSELVVEFPDTEWLECDHFEDALSAVESPLESSAPIVVLRLQPDTKVMLDAIARFLALCNQLVVAGKTVVLDFSGCKNTSSYLDRLGFYEHLKPEVEVLPQRPSKSRAAAYRGQSSRLVELAAISVDEPDEDIPDRLHQVLEQQYENNAPVGLHTILTELFSNVHEHSQSKLPGFAALQLYKGRKPHIRTVFSDSGKGILATIKPALTKTLRTELGAETDDMTPDLGIALIERLFTQGGISRFRKGGRGLGLRNTGRFADKFAGSITVRQDTYEVTIHFGGDEHEFAARTGLNKLEGTHICVKFELTRWLKPH</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-01-15" modified="2022-08-03" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LI38</accession>
    <name>RL4_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">50S ribosomal protein L4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rplD</name>
        <name type="ordered locus">Rmet_3316</name>
    </gene>
    <organism>
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">One of the primary rRNA binding proteins, this protein initially binds near the 5'-end of the 23S rRNA. It is important during the early stages of 50S assembly. It makes multiple contacts with different domains of the 23S rRNA in the assembled 50S subunit and ribosome.</text>
    </comment>
    <comment type="function">
        <text evidence="1">Forms part of the polypeptide exit tunnel.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the 50S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL4 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10188.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008642923.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q1LI38"/>
    <dbReference type="SMR" id="Q1LI38"/>
    <dbReference type="STRING" id="266264.Rmet_3316"/>
    <dbReference type="EnsemblBacteria" id="ABF10188">
        <property type="protein sequence ID" value="ABF10188"/>
        <property type="gene ID" value="Rmet_3316"/>
    </dbReference>
    <dbReference type="GeneID" id="60826601"/>
    <dbReference type="KEGG" id="rme:Rmet_3316"/>
    <dbReference type="eggNOG" id="COG0088">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041575_5_2_4"/>
    <dbReference type="OMA" id="PQVHILE"/>
    <dbReference type="OrthoDB" id="1572673at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1370.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01328_B">
        <property type="entry name" value="Ribosomal_L4_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002136">
        <property type="entry name" value="Ribosomal_L4/L1e"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023574">
        <property type="entry name" value="Ribosomal_L4_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013005">
        <property type="entry name" value="Ribosomal_uL4/L1e"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10746">
        <property type="entry name" value="PTHR10746"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00573">
        <property type="entry name" value="Ribosomal_L4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52166">
        <property type="entry name" value="SSF52166"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03953">
        <property type="entry name" value="rplD_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <feature type="chain" id="PRO_1000052477" description="50S ribosomal protein L4">
        <location>
            <begin position="1"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="43"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="46"/>
            <end position="62"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="206" mass="22854" checksum="1D910CB9A5BBEBF8" modified="2006-05-30" version="1">MELKLLQDNGQIGAGVAASAEVFGRDYNEALVHQIVVAYQANARSGNRKQKDREEVKHTTKKPWRQKGTGRARAGMSSSPLWRGGGRIFPNSPEENFSQKVNKKMFRAGMRSIYSQLAREGRINVVDGFAVDAPKTKLLADKFKAMGLDSVLIITDNLDENLYLASRNLPNVAVVEPRHADPLSLVHYKKVLVTKAAVAQIEELLK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-03-18" modified="2022-08-03" version="103" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LIH4</accession>
    <name>TRPD_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Anthranilate phosphoribosyltransferase</fullName>
            <ecNumber evidence="1">2.4.2.18</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">trpD</name>
        <name type="ordered locus">Rmet_3180</name>
    </gene>
    <organism>
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the transfer of the phosphoribosyl group of 5-phosphorylribose-1-pyrophosphate (PRPP) to anthranilate to yield N-(5'-phosphoribosyl)-anthranilate (PRA).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>diphosphate + N-(5-phospho-beta-D-ribosyl)anthranilate = 5-phospho-alpha-D-ribose 1-diphosphate + anthranilate</text>
            <dbReference type="Rhea" id="RHEA:11768"/>
            <dbReference type="ChEBI" id="CHEBI:16567"/>
            <dbReference type="ChEBI" id="CHEBI:18277"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <dbReference type="EC" id="2.4.2.18"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="1">Binds 2 magnesium ions per monomer.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-tryptophan biosynthesis; L-tryptophan from chorismate: step 2/5.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the anthranilate phosphoribosyltransferase family.</text>
    </comment>
    <comment type="sequence caution" evidence="2">
        <conflict type="erroneous initiation">
            <sequence resource="EMBL-CDS" id="ABF10052" version="1"/>
        </conflict>
        <text>Extended N-terminus.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.18" evidence="1"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10052.1"/>
        <property type="status" value="ALT_INIT"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q1LIH4"/>
    <dbReference type="SMR" id="Q1LIH4"/>
    <dbReference type="STRING" id="266264.Rmet_3180"/>
    <dbReference type="EnsemblBacteria" id="ABF10052">
        <property type="protein sequence ID" value="ABF10052"/>
        <property type="gene ID" value="Rmet_3180"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3180"/>
    <dbReference type="eggNOG" id="COG0547">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_034315_2_1_4"/>
    <dbReference type="UniPathway" id="UPA00035">
        <property type="reaction ID" value="UER00041"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004048">
        <property type="term" value="F:anthranilate phosphoribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000162">
        <property type="term" value="P:tryptophan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1030.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00211">
        <property type="entry name" value="TrpD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005940">
        <property type="entry name" value="Anthranilate_Pribosyl_Tfrase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000312">
        <property type="entry name" value="Glycosyl_Trfase_fam3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017459">
        <property type="entry name" value="Glycosyl_Trfase_fam3_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036320">
        <property type="entry name" value="Glycosyl_Trfase_fam3_N_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035902">
        <property type="entry name" value="Nuc_phospho_transferase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43285">
        <property type="entry name" value="PTHR43285"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02885">
        <property type="entry name" value="Glycos_trans_3N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00591">
        <property type="entry name" value="Glycos_transf_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47648">
        <property type="entry name" value="SSF47648"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52418">
        <property type="entry name" value="SSF52418"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01245">
        <property type="entry name" value="trpD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword id="KW-0328">Glycosyltransferase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <keyword id="KW-0822">Tryptophan biosynthesis</keyword>
    <feature type="chain" id="PRO_0000325453" description="Anthranilate phosphoribosyltransferase">
        <location>
            <begin position="1"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="84"/>
        </location>
        <ligand>
            <name>5-phospho-alpha-D-ribose 1-diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="84"/>
        </location>
        <ligand>
            <name>anthranilate</name>
            <dbReference type="ChEBI" id="CHEBI:16567"/>
            <label>1</label>
        </ligand>
    </feature>
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        <location>
            <begin position="87"/>
            <end position="88"/>
        </location>
        <ligand>
            <name>5-phospho-alpha-D-ribose 1-diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="92"/>
        </location>
        <ligand>
            <name>5-phospho-alpha-D-ribose 1-diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="94"/>
            <end position="97"/>
        </location>
        <ligand>
            <name>5-phospho-alpha-D-ribose 1-diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="96"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
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        <location>
            <begin position="112"/>
            <end position="120"/>
        </location>
        <ligand>
            <name>5-phospho-alpha-D-ribose 1-diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
        </ligand>
    </feature>
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        <location>
            <position position="115"/>
        </location>
        <ligand>
            <name>anthranilate</name>
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            <label>1</label>
        </ligand>
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        <location>
            <position position="124"/>
        </location>
        <ligand>
            <name>5-phospho-alpha-D-ribose 1-diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
        </ligand>
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    <feature type="binding site" evidence="1">
        <location>
            <position position="170"/>
        </location>
        <ligand>
            <name>anthranilate</name>
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            <label>2</label>
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        <location>
            <position position="229"/>
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        <location>
            <position position="230"/>
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        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="342" mass="36748" checksum="ACC5506712E5359F" modified="2008-03-18" version="2">MSITPQEALTRCIEHREIFHDEMLHLMRQIMQGQISPVMAAAILTGLRVKKETIGEISAAAQVMREFANHVTVKDRENFVDIVGTGGDGSHTFNISTASMFVAAAAGAKIAKHGNRGVSSKSGSADVLEALGVNIMLTPEQVGECIEQTGIGFMFAPTHHPAMKNVAPIRKEMGVRTIFNILGPLTNPADAPNILMGVFHPDLVGIQVRVMQRLGAQHAIVVYGKDGMDEVSLGAATLVGELKDGEVREYEIHPEDFGLSMISNRGLKVADAVESKEMLLEALSDVPGTPREIVSLNAGTALYAANVASSIEDGIRRAREAIASGAAREKLDQFVRATQQFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="66" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LIU3</accession>
    <name>Q1LIU3_CUPMC</name>
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        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
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        <name evidence="1" type="ordered locus">Rmet_3061</name>
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        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
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    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
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    </reference>
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        <property type="protein sequence ID" value="ABF09933.1"/>
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    </dbReference>
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    <dbReference type="OMA" id="FCDDPKS"/>
    <dbReference type="OrthoDB" id="1659739at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF09933.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="227" mass="24714" checksum="B60854B4DEB2D506" modified="2006-05-30" version="1">MSRRMPSDPIRLREEIAQAAARMIAEDGADYATAKRKAARQILGDVRVAGEWLPDNEMIEDEVRAYQALFHGDHQPRILALMRQLALLAMDDLAAFRPYLVGAVLNGTATEHSDVYIQCFCDSPKDVALHLLNAGVDFDTSESRHFAGRGDVETLSFLWRGQWPATREARTLAGEVRATLGAPVGIHLALYDADDARGAVKADASGRVARADADAVRTLIASTDAGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="95" xmlns="http://uniprot.org/uniprot">
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        <name evidence="9" type="ordered locus">Rmet_2864</name>
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        <lineage>
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    <reference evidence="10" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
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                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
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                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
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    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
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        <property type="entry name" value="BCA_ABC_TP_C"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-1278">Translocase</keyword>
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        <location>
            <begin position="8"/>
            <end position="255"/>
        </location>
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            <begin position="40"/>
            <end position="47"/>
        </location>
        <ligand>
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    </feature>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ABF09737.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="258" mass="27967" checksum="3F1E56E80E099131" modified="2006-05-30" version="1">MSNSDFLLSVQGVNKRFGGLQALSDVGLQINPGEIYGLIGPNGAGKTTFFNVITGLYTPDSGEFVLGGKPYQPTAVHEVAKAGIARTFQNIRLFGDMTALENVMVGRHVRTKAGLFGAIFRPPAVRQEEEAVEDMAHDLLEYVGIGKYANFTSRNLSYGHQRRLEIARALATEPKLLALDEPAAGMNATEKVELRGLLEKIKNDGKTILLIEHDVKLVMGLCNRLTVLDYGKVIAQGLPHEVQNNPAVIEAYLGAAAH</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="61" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LJP1</accession>
    <name>Q1LJP1_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name evidence="1" type="ordered locus">Rmet_2762</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
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    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09635.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008651781.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2762"/>
    <dbReference type="EnsemblBacteria" id="ABF09635">
        <property type="protein sequence ID" value="ABF09635"/>
        <property type="gene ID" value="Rmet_2762"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2762"/>
    <dbReference type="HOGENOM" id="CLU_180794_2_1_4"/>
    <dbReference type="OMA" id="HIRKYYR"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014177">
        <property type="entry name" value="Formate_DH_TAT-contain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006311">
        <property type="entry name" value="TAT_signal"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036704">
        <property type="entry name" value="UCP036704"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02811">
        <property type="entry name" value="formate_TAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51318">
        <property type="entry name" value="TAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF09635.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="71" mass="7321" checksum="228A3AC6A489FE91" modified="2006-05-30" version="1">MEDKQPRVARRTFLAGAGVVAAAGAAVVATRGQPALAEAVAAKAEPADPAPGSDGYRVSAHIRKYYRTTLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="65" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LJZ6</accession>
    <name>Q1LJZ6_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">elaB</name>
        <name evidence="12" type="ordered locus">Rmet_2655</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell inner membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the ElaB/YgaM/YqjD family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09530.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_006162945.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2655"/>
    <dbReference type="EnsemblBacteria" id="ABF09530">
        <property type="protein sequence ID" value="ABF09530"/>
        <property type="gene ID" value="Rmet_2655"/>
    </dbReference>
    <dbReference type="GeneID" id="60820910"/>
    <dbReference type="KEGG" id="rme:Rmet_2655"/>
    <dbReference type="PATRIC" id="fig|381666.6.peg.3220"/>
    <dbReference type="eggNOG" id="COG4575">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_132623_0_2_4"/>
    <dbReference type="OMA" id="KDQAFSE"/>
    <dbReference type="OrthoDB" id="2003760at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043022">
        <property type="term" value="F:ribosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043605">
        <property type="entry name" value="DUF883_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043604">
        <property type="entry name" value="DUF883_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010279">
        <property type="entry name" value="YqjD/ElaB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35893">
        <property type="entry name" value="PTHR35893"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05957">
        <property type="entry name" value="DUF883"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19029">
        <property type="entry name" value="DUF883_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="70"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="domain" description="DUF883" evidence="10">
        <location>
            <begin position="1"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="domain" description="DUF883_C" evidence="11">
        <location>
            <begin position="62"/>
            <end position="91"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004377"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010423"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF05957"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF19029"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ABF09530.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="91" mass="9726" checksum="5D01902E4B8670E4" modified="2006-05-30" version="1">MSDVKTVLSDAEELLKQAASTTGEKAAELRERGMGLLKQAKEKAQDLQDAVVTKSKAAARATDDYVHDHPWRAVGVAAGVGLLIGLLLNRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LK15</accession>
    <name>Q1LK15_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Ankyrin repeat harboring signal peptide protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_2634</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09511.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2634"/>
    <dbReference type="EnsemblBacteria" id="ABF09511">
        <property type="protein sequence ID" value="ABF09511"/>
        <property type="gene ID" value="Rmet_2634"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2634"/>
    <dbReference type="eggNOG" id="COG0666">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000134_18_13_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="SSF48403"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="1">
        <location>
            <begin position="106"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="1">
        <location>
            <begin position="139"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="1">
        <location>
            <begin position="172"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="1">
        <location>
            <begin position="205"/>
            <end position="242"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF09511.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="269" mass="28272" checksum="62CBE3845D9D2F57" modified="2006-05-30" version="1">MTRSQTRPRRQALAEILGLMGLLVAGGLLAGQGGQTLARSVGGLGGASAPMPSRPWHGSNHTPLPSRGDGMAALDHNLITAASIGDLDLVNRLLKAGASAQATDERGRTALLAAVYNRRGDVMLVLIQAGADVNRKDDEGNSPFLLAAATNQIDVVRLALSHGADLNSTDRYDGTALIAASQHGNVEIVKLLISAGVPVDRVNQLGWTALLEAIILGDGSSRYEDIVQALLDAGADANLADREGVTPSRHARERGYKTMVRMLMRARGH</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="99" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LK85</accession>
    <name>Q1LK85_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="11">ATP-dependent RNA helicase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">rhlE2</name>
        <name evidence="11" type="ordered locus">Rmet_2564</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="6">Belongs to the DEAD box helicase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09441.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517144.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2564"/>
    <dbReference type="EnsemblBacteria" id="ABF09441">
        <property type="protein sequence ID" value="ABF09441"/>
        <property type="gene ID" value="Rmet_2564"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2564"/>
    <dbReference type="eggNOG" id="COG0513">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003041_28_3_4"/>
    <dbReference type="OMA" id="YDIELYQ"/>
    <dbReference type="OrthoDB" id="643629at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000629">
        <property type="entry name" value="RNA-helicase_DEAD-box_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014014">
        <property type="entry name" value="RNA_helicase_DEAD_Q_motif"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00039">
        <property type="entry name" value="DEAD_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51195">
        <property type="entry name" value="Q_MOTIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 6 11" id="KW-0347">Helicase</keyword>
    <keyword evidence="2 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Q_MOTIF" evidence="10">
        <location>
            <begin position="49"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="8">
        <location>
            <begin position="80"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="9">
        <location>
            <begin position="300"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="113"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="439"/>
            <end position="634"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Q motif" evidence="5">
        <location>
            <begin position="49"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="115"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="489"/>
            <end position="531"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="532"/>
            <end position="562"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="567"/>
            <end position="581"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="597"/>
            <end position="617"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ABF09441.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="634" mass="68795" checksum="E03176291B0E0416" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="69" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LKF2</accession>
    <name>Q1LKF2_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Spermidine synthase-like protein</fullName>
            <ecNumber evidence="2">2.5.1.16</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_2497</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.5.1.16" evidence="2"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09374.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2497"/>
    <dbReference type="EnsemblBacteria" id="ABF09374">
        <property type="protein sequence ID" value="ABF09374"/>
        <property type="gene ID" value="Rmet_2497"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2497"/>
    <dbReference type="eggNOG" id="COG0421">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_060070_0_0_4"/>
    <dbReference type="OMA" id="EYVQRMM"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004766">
        <property type="term" value="F:spermidine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="25"/>
            <end position="52"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF09374.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="310" mass="34474" checksum="E8F053CDA7C42463" modified="2006-05-30" version="1">MIRALSALYSSMTLLKRKSIEAVASRRPARGERETSRRKLSGDAGDVDNAGEKRMTPRFAPVTFSEMDGVRYLHFGTEWVQGAMRLRKPDAIELEYAQQMMAWLLFLSPSAADFHVAQLGLGAAALTKFCHRQFARAKVTAVELNPAVIVAGRSMFGLREDDARLSVREQDAWDFVMDGAQTASIDALQVDLYDATARGPVLDTTAFYRACRRVLKAPGVMTINLFGDHESFPKNIVRICDAFDNRVLVFPEVHDGNIIALAFNGPAIDVSWETLEARAEVVEATTKLPAKDWVKKLRAANARQEERLAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="99" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LKM4</accession>
    <name>Q1LKM4_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">RNA polymerase sigma factor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">rpoE</name>
        <name evidence="5" type="ordered locus">Rmet_2425</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the sigma-70 factor family. ECF subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09302.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008644807.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2425"/>
    <dbReference type="EnsemblBacteria" id="ABF09302">
        <property type="protein sequence ID" value="ABF09302"/>
        <property type="gene ID" value="Rmet_2425"/>
    </dbReference>
    <dbReference type="GeneID" id="60821101"/>
    <dbReference type="KEGG" id="rme:Rmet_2425"/>
    <dbReference type="eggNOG" id="COG1595">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047691_3_0_4"/>
    <dbReference type="OMA" id="LVDMQGY"/>
    <dbReference type="OrthoDB" id="1914729at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016987">
        <property type="term" value="F:sigma factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006352">
        <property type="term" value="P:DNA-templated transcription, initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006950">
        <property type="term" value="P:response to stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039425">
        <property type="entry name" value="RNA_pol_sigma-70-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014284">
        <property type="entry name" value="RNA_pol_sigma-70_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000838">
        <property type="entry name" value="RNA_pol_sigma70_ECF_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007627">
        <property type="entry name" value="RNA_pol_sigma70_r2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013249">
        <property type="entry name" value="RNA_pol_sigma70_r4_t2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014286">
        <property type="entry name" value="RNA_pol_sigma70_RpoE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013325">
        <property type="entry name" value="RNA_pol_sigma_r2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013324">
        <property type="entry name" value="RNA_pol_sigma_r3/r4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43133">
        <property type="entry name" value="PTHR43133"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04542">
        <property type="entry name" value="Sigma70_r2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08281">
        <property type="entry name" value="Sigma70_r4_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88659">
        <property type="entry name" value="SSF88659"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88946">
        <property type="entry name" value="SSF88946"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02939">
        <property type="entry name" value="RpoE_Sigma70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02937">
        <property type="entry name" value="sigma70-ECF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01063">
        <property type="entry name" value="SIGMA70_ECF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0731">Sigma factor</keyword>
    <keyword evidence="2" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Sigma70_r2" evidence="3">
        <location>
            <begin position="25"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="domain" description="Sigma70_r4_2" evidence="4">
        <location>
            <begin position="134"/>
            <end position="182"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU000716"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF04542"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF08281"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABF09302.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="199" mass="22755" checksum="491424E8D7816E4A" modified="2006-05-30" version="1">MSEREADQLLVERVQQGDKRAFELLVTKYHRKIIRLISRLVRDPAEVEDVAQDAFIKAYRALPQFRGESAFYTWLYRIAVNTAKNYLATQGRRPEASSDIDAEEAETFADGEQLRDINTPESMLHTRQVADTVNRAMEALPEELRTAITLREIEGLSYEEIAEAMGCPIGTVRSRIFRAREAIAEKLRPLLGTAEGKRW</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="113" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LKS0</accession>
    <name>Q1LKS0_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Metal transporter ATPase</fullName>
            <ecNumber evidence="8">3.6.3.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">ctpA1</name>
        <name evidence="8" type="ordered locus">Rmet_2379</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cell membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IB subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.3.-" evidence="8"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09256.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517003.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2379"/>
    <dbReference type="EnsemblBacteria" id="ABF09256">
        <property type="protein sequence ID" value="ABF09256"/>
        <property type="gene ID" value="Rmet_2379"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2379"/>
    <dbReference type="eggNOG" id="COG2217">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001771_0_3_4"/>
    <dbReference type="OMA" id="SWVLYLN"/>
    <dbReference type="OrthoDB" id="237367at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019829">
        <property type="term" value="F:ATPase-coupled cation transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00371">
        <property type="entry name" value="HMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1110.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023299">
        <property type="entry name" value="ATPase_P-typ_cyto_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018303">
        <property type="entry name" value="ATPase_P-typ_P_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023298">
        <property type="entry name" value="ATPase_P-typ_TM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008250">
        <property type="entry name" value="ATPase_P-typ_transduc_dom_A_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017969">
        <property type="entry name" value="Heavy-metal-associated_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006121">
        <property type="entry name" value="HMA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036163">
        <property type="entry name" value="HMA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027256">
        <property type="entry name" value="P-typ_ATPase_IB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001757">
        <property type="entry name" value="P_typ_ATPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00403">
        <property type="entry name" value="HMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55008">
        <property type="entry name" value="SSF55008"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SSF56784"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81653">
        <property type="entry name" value="SSF81653"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81665">
        <property type="entry name" value="SSF81665"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01525">
        <property type="entry name" value="ATPase-IB_hvy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01494">
        <property type="entry name" value="ATPase_P-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00154">
        <property type="entry name" value="ATPASE_E1_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01047">
        <property type="entry name" value="HMA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50846">
        <property type="entry name" value="HMA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="108"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="134"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="168"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="193"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="355"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="388"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="692"/>
            <end position="709"/>
        </location>
    </feature>
    <feature type="domain" description="HMA" evidence="7">
        <location>
            <begin position="22"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU362081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ABF09256.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="752" mass="78711" checksum="D8F12DFE48E11B68" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LL81</accession>
    <name>Q1LL81_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Cysteine dioxygenase type I</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_2216</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the cysteine dioxygenase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09095.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516923.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2216"/>
    <dbReference type="EnsemblBacteria" id="ABF09095">
        <property type="protein sequence ID" value="ABF09095"/>
        <property type="gene ID" value="Rmet_2216"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2216"/>
    <dbReference type="eggNOG" id="COG5553">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_118472_1_0_4"/>
    <dbReference type="OMA" id="DHRVWGL"/>
    <dbReference type="OrthoDB" id="1571420at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016702">
        <property type="term" value="F:oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010300">
        <property type="entry name" value="CDO_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011051">
        <property type="entry name" value="RmlC_Cupin_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12918">
        <property type="entry name" value="PTHR12918"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05995">
        <property type="entry name" value="CDO_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51182">
        <property type="entry name" value="SSF51182"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="2" id="KW-0408">Iron</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="2">
        <location>
            <position position="93"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="95"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="146"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR610300-51"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF09095.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="206" mass="22716" checksum="8AF0A77186587EEE" modified="2006-05-30" version="1">MSTSPTPAQALKPLRDFITAFTALLDQHPDEARILDEGGSLLAKLVARDDWLPDAYAQPHPEYYQQMLLHCDSAERFSIVSFVWGPGQRTPIHDHTVWGLIGMLRGSEYAQPFEIDAESTPQPQGEATRLEPGDVEAVSPTVGDIHRVHNAYDDRVSISIHVYGANIGAVRRHVFPETGGSKAFISGYSNSTLPNPWDRSKDPIAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="116" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LLJ2</accession>
    <name>Q1LLJ2_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Histidine--tRNA ligase</fullName>
            <ecNumber evidence="9">6.1.1.21</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Histidyl-tRNA synthetase</fullName>
            <shortName evidence="9">HisRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9 11" type="primary">hisS</name>
        <name evidence="11" type="ordered locus">Rmet_2105</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 9">
            <text>ATP + L-histidine + tRNA(His) = AMP + diphosphate + H(+) + L-histidyl-tRNA(His)</text>
            <dbReference type="Rhea" id="RHEA:17313"/>
            <dbReference type="Rhea" id="RHEA-COMP:9665"/>
            <dbReference type="Rhea" id="RHEA-COMP:9689"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57595"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78527"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.21"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="3 9">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the class-II aminoacyl-tRNA synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.21" evidence="9"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08984.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516818.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2105"/>
    <dbReference type="EnsemblBacteria" id="ABF08984">
        <property type="protein sequence ID" value="ABF08984"/>
        <property type="gene ID" value="Rmet_2105"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2105"/>
    <dbReference type="eggNOG" id="COG0124">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025113_1_1_4"/>
    <dbReference type="OMA" id="CDFDFIG"/>
    <dbReference type="OrthoDB" id="277998at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004821">
        <property type="term" value="F:histidine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006427">
        <property type="term" value="P:histidyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00773">
        <property type="entry name" value="HisRS-like_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00859">
        <property type="entry name" value="HisRS_anticodon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.800">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00127">
        <property type="entry name" value="His_tRNA_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004154">
        <property type="entry name" value="Anticodon-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036621">
        <property type="entry name" value="Anticodon-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015807">
        <property type="entry name" value="His-tRNA-ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041715">
        <property type="entry name" value="HisRS-like_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004516">
        <property type="entry name" value="HisRS/HisZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033656">
        <property type="entry name" value="HisRS_anticodon"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43707">
        <property type="entry name" value="PTHR43707"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03129">
        <property type="entry name" value="HGTP_anticodon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13393">
        <property type="entry name" value="tRNA-synt_His"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001549">
        <property type="entry name" value="His-tRNA_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="SSF55681"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00442">
        <property type="entry name" value="hisS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 9 11" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="6 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 9 11" id="KW-0436">Ligase</keyword>
    <keyword evidence="5 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7 9" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AA_TRNA_LIGASE_II" evidence="10">
        <location>
            <begin position="28"/>
            <end position="331"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ABF08984.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="447" mass="49695" checksum="86B4999E6074B71C" modified="2006-05-30" version="1">MSEDKTKAVKALQGVKGMNDMLPTDAPLWDMFESAARSMLRAYGYQQIRTPIVEHTQLFVRGIGEVTDIVEKEMYSFTDSLNGENLTLRPEGTAAAVRSTIEHNLLYDGPKRLWYIGPMFRHERPQRGRYRQFHQLGAEALGFAGPDVDAEIILMCQRLWDDLGLTNVRLELNSLGQAHERAAHREQLIKYLEGFQDILDEDSKRRLYTNPLRVLDTKNPALQEMAANAPKLIDFLGEESLAHFEGVQRILLANNIPFKINPRLVRGLDYYNLTVFEWITDKLGAQGTIAGGGRYDPLIQQMGGKPAPACGWAMGIERIIELIREENLVPDTQGCDVYMVHQGELANQQAMIAAERLRDAGLDVVLHATPDGKSGSFKSQMKRADASGASFAVIIGDDEIAAGTVQVKQLRGGNVAEGGQQTTVPAEQVVEFIIDAMVDEIEGDDEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LLK4</accession>
    <name>Q1LLK4_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transcriptional regulator, BadM/Rrf2 family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_2093</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08972.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516806.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2093"/>
    <dbReference type="EnsemblBacteria" id="ABF08972">
        <property type="protein sequence ID" value="ABF08972"/>
        <property type="gene ID" value="Rmet_2093"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2093"/>
    <dbReference type="eggNOG" id="COG1959">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_107144_4_0_4"/>
    <dbReference type="OMA" id="ACWYFKS"/>
    <dbReference type="OrthoDB" id="1794390at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000944">
        <property type="entry name" value="Tscrpt_reg_Rrf2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33221">
        <property type="entry name" value="PTHR33221"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02082">
        <property type="entry name" value="Rrf2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51197">
        <property type="entry name" value="HTH_RRF2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193652" evidence="1">
        <location>
            <begin position="21"/>
            <end position="144"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF08972.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="144" mass="14972" checksum="CF06DA153A4D67B9" modified="2006-05-30" version="1">MSTSSRFAVAVHILTLLAQAEGPVPSSMIAGSVGTNPALIRRMIGALTEAGFVTTTMGSTGGAMLTRPASQITLLEVFQATETTALITLHQSAPNPACLVGREITGTLQVVADRAQAAMDATLAEITIASMLGEVEHAARRRKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="67" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LLP0</accession>
    <name>Q1LLP0_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Hemagglutinin-related transmembrane protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_2057</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08936.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516768.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2057"/>
    <dbReference type="EnsemblBacteria" id="ABF08936">
        <property type="protein sequence ID" value="ABF08936"/>
        <property type="gene ID" value="Rmet_2057"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2057"/>
    <dbReference type="eggNOG" id="ENOG502Z9CN">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_034937_0_0_4"/>
    <dbReference type="OMA" id="IDANYNA"/>
    <dbReference type="OrthoDB" id="751525at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019501">
        <property type="entry name" value="Peptidase_M30_hyicolysin"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10460">
        <property type="entry name" value="Peptidase_M30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193289" evidence="2">
        <location>
            <begin position="29"/>
            <end position="561"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="30"/>
            <end position="62"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF08936.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="561" mass="60138" checksum="C1D477BEA35D0FF0" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-04-07" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LLV9</accession>
    <name>Q1LLV9_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_1988</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08867.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516708.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1988"/>
    <dbReference type="EnsemblBacteria" id="ABF08867">
        <property type="protein sequence ID" value="ABF08867"/>
        <property type="gene ID" value="Rmet_1988"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1988"/>
    <dbReference type="HOGENOM" id="CLU_173874_0_0_4"/>
    <dbReference type="OMA" id="KWTARYC"/>
    <dbReference type="OrthoDB" id="1880106at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF08867.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="86" mass="9593" checksum="991709D5F240F978" modified="2006-05-30" version="1">MGSEEPFGEVFEGCRVIWVVEQLQDGKWTARYCWHAGTSAESGRALQRDVLNGKSKRLLALCPTEAETIAAIKEAVLLEARWQGSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="69" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LM51</accession>
    <name>Q1LM51_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_1896</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08775.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516627.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1896"/>
    <dbReference type="EnsemblBacteria" id="ABF08775">
        <property type="protein sequence ID" value="ABF08775"/>
        <property type="gene ID" value="Rmet_1896"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1896"/>
    <dbReference type="eggNOG" id="COG1942">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_148073_0_0_4"/>
    <dbReference type="OMA" id="QIVHQHP"/>
    <dbReference type="OrthoDB" id="2013680at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.429.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037479">
        <property type="entry name" value="Tauto_MSAD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014347">
        <property type="entry name" value="Tautomerase/MIF_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38460">
        <property type="entry name" value="PTHR38460"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14552">
        <property type="entry name" value="Tautomerase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55331">
        <property type="entry name" value="SSF55331"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF08775.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="136" mass="14835" checksum="36B4FCC670F72E65" modified="2006-05-30" version="1">MPMTHVSMRKGQPAAFRAAVLEAIHESLQKSLDVHPEAFFMTISEHEDANFHANMTYPFVRSPNLLLIQITLTAGRSTGDKQRFYRNLVARLEAGSGIKPADVFINLIEVASENWSPGNGLPIATALQPDAAHGPG</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LM70</accession>
    <name>Q1LM70_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">D-amino acid dehydrogenase</fullName>
            <ecNumber evidence="2">1.4.99.1</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">dadA</name>
        <name evidence="2" type="ordered locus">Rmet_1877</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EC" id="1.4.99.1" evidence="2"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08756.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516602.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1877"/>
    <dbReference type="EnsemblBacteria" id="ABF08756">
        <property type="protein sequence ID" value="ABF08756"/>
        <property type="gene ID" value="Rmet_1877"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1877"/>
    <dbReference type="eggNOG" id="COG0665">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007884_9_2_4"/>
    <dbReference type="OMA" id="VWLNLGH"/>
    <dbReference type="OrthoDB" id="573710at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006076">
        <property type="entry name" value="FAD-dep_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01266">
        <property type="entry name" value="DAO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="SSF51905"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DAO" evidence="1">
        <location>
            <begin position="2"/>
            <end position="400"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF08756.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="429" mass="46713" checksum="67E4F87D8D27B8B7" modified="2006-05-30" version="1">MHVIIIGAGAIGVCSAWYLRQAGFEVTVLERRGAPAQEASFGNAGMIAPGYVTPWAAPGMPGKIVRSLFQHASPVVFRPSVDPAMWRWIVRWLRECNPESYRTNKLRMQRVAFYSRACLHELRSALEIEYEQSRGYLQLFRTQRDLDLASPALALLRENNVPHQLIDAAGCRRIEPGLTDDTPLAGGLHLPEDESGNCPIFVRRLHQHAEAAGVRFRFQTEVARLRRTDGRLTVELAALRGHAREVLDADRVLLAAGVGSETLLRPLGLRLPLYPVKGYSATVMVTDELQAPLGALMDESFKVAITRMGNRLRVAGTAELGSRKLDLRPAALQTLIKVARDWFPVAGHYNAATLWAGARPMLPDGPPLIGATRVPGLFLNLGHGSTGWAMSCGSGKIAADQIAASAGQGDGPAIDMDGLTPERYRLGPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LM80</accession>
    <name>Q1LM80_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Regulator of penicillin binding proteins and beta lactamase transcription (Morphogene)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">bolA</name>
        <name evidence="2" type="ordered locus">Rmet_1867</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the BolA/IbaG family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08746.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516592.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1867"/>
    <dbReference type="EnsemblBacteria" id="ABF08746">
        <property type="protein sequence ID" value="ABF08746"/>
        <property type="gene ID" value="Rmet_1867"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1867"/>
    <dbReference type="eggNOG" id="COG0271">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_109462_2_1_4"/>
    <dbReference type="OMA" id="TRVEIHR"/>
    <dbReference type="OrthoDB" id="1981704at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002634">
        <property type="entry name" value="BolA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036065">
        <property type="entry name" value="BolA-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01722">
        <property type="entry name" value="BolA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF003113">
        <property type="entry name" value="BolA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82657">
        <property type="entry name" value="SSF82657"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU003860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF08746.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="104" mass="11215" checksum="113C81513DB0E6F7" modified="2006-05-30" version="1">MAADPATIEALLQAALDPTRLAVRDDSAAHAGHAGAASGGGHYDVLIVSERFGGHSRVARHRMVYDALRELFPSQIHALAVNAYTDQEYQSREATRDATRNSQT</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LMA0</accession>
    <name>Q1LMA0_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Glutathione S-transferase</fullName>
            <ecNumber evidence="2">2.5.1.18</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_1847</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.5.1.18" evidence="2"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08726.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516576.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1847"/>
    <dbReference type="EnsemblBacteria" id="ABF08726">
        <property type="protein sequence ID" value="ABF08726"/>
        <property type="gene ID" value="Rmet_1847"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1847"/>
    <dbReference type="eggNOG" id="COG0625">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039745_2_0_4"/>
    <dbReference type="OMA" id="ADFSVYH"/>
    <dbReference type="OrthoDB" id="1819702at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004364">
        <property type="term" value="F:glutathione transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006749">
        <property type="term" value="P:glutathione metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036282">
        <property type="entry name" value="Glutathione-S-Trfase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004045">
        <property type="entry name" value="Glutathione_S-Trfase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13417">
        <property type="entry name" value="GST_N_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47616">
        <property type="entry name" value="SSF47616"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="SSF52833"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50404">
        <property type="entry name" value="GST_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="GST N-terminal" evidence="1">
        <location>
            <begin position="2"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF08726.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="310" mass="33857" checksum="34CB738068535026" modified="2006-05-30" version="1">MPDIILHQYATSPFSEKIRLLLGAKDLSWCAVEIPPILPKPDLVALTGGYRRTPVMQIGADIYCDTALICEVLDRMAPLPPLYPPDQAAASSVMAAWFDSALFTASVTYTMQPAGVQAMLAHLTPDQIKAFVADRKAMRGDTNALRMPLPEADAMLHETFERLQEQFAAGVLHVAGPMLSVADFSLYHNIWFIRRATALAAILDAYPLVKEWYARMQAYGHGKVRVIPAEDALAEALHNDPLAHDTTIQPDSGFAAGDAVTVTPTDYAKDPVAGVLMTLSADVVTIRRQDPRAGAVNVHFPRQHYQVQAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LMD8</accession>
    <name>Q1LMD8_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Phosphonopyruvate decarboxylase</fullName>
            <ecNumber evidence="4">4.1.1.82</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">ppd</name>
        <name evidence="4" type="ordered locus">Rmet_1809</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EC" id="4.1.1.82" evidence="4"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08688.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516538.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1809"/>
    <dbReference type="EnsemblBacteria" id="ABF08688">
        <property type="protein sequence ID" value="ABF08688"/>
        <property type="gene ID" value="Rmet_1809"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1809"/>
    <dbReference type="eggNOG" id="COG0028">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG4032">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_042853_0_0_4"/>
    <dbReference type="OMA" id="PILASWR"/>
    <dbReference type="OrthoDB" id="1219810at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033980">
        <property type="term" value="F:phosphonopyruvate decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030976">
        <property type="term" value="F:thiamine pyrophosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032923">
        <property type="term" value="P:organic phosphonate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044281">
        <property type="term" value="P:small molecule metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017684">
        <property type="entry name" value="Phosphono-pyrv_decarboxylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012001">
        <property type="entry name" value="Thiamin_PyroP_enz_TPP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000399">
        <property type="entry name" value="TPP-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011766">
        <property type="entry name" value="TPP_enzyme-bd_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02775">
        <property type="entry name" value="TPP_enzyme_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02776">
        <property type="entry name" value="TPP_enzyme_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="SSF52518"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03297">
        <property type="entry name" value="Ppyr-DeCO2ase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00187">
        <property type="entry name" value="TPP_ENZYMES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0456">Lyase</keyword>
    <keyword evidence="4" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TPP_enzyme_N" evidence="3">
        <location>
            <begin position="7"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="domain" description="TPP_enzyme_C" evidence="2">
        <location>
            <begin position="237"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="180"/>
            <end position="199"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF02776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF08688.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="399" mass="41832" checksum="4AD5F74CAE9EBAFB" modified="2006-05-30" version="1">MIEAVQFVEAARERGFEWYAGVPCSYLTPFINYVVQDPSLHYVSAANEGDAVAFIAGVTQGARNGVRGITMMQNSGLGNAVSPLTSLTWTFRLPQLLIVTWRGQPGGASDEPQHALMGPVTPAMLDTMEIPWELFPTEPDAVGPALDRAIAHMDATGRPYALIMQKGSVAPYPLKTQTPPVARAKATPQVSRSGATPLPSRQEALQRVIAHTPADSTVVLASTGFCGRELYALDDRPNQLYMVGSMGCLTPFALGLAMARPDLKVVAVDGDGAALMRMGVFATLGAYGPANLTHVLLDNNAHDSTGGQATVSHNVSFAGVAAACGYASAIEGDDLDMLDRVLASAATATSGPNFVCLQTRAGTPDGLPRPSVTPVEVKTRLGRQIGADQGHAGEKHAAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LMM8</accession>
    <name>Q1LMM8_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Taurine dioxygenase</fullName>
            <ecNumber evidence="3">1.14.11.17</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">tauD</name>
        <name evidence="3" type="ordered locus">Rmet_1717</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EC" id="1.14.11.17" evidence="3"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08598.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516450.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1717"/>
    <dbReference type="EnsemblBacteria" id="ABF08598">
        <property type="protein sequence ID" value="ABF08598"/>
        <property type="gene ID" value="Rmet_1717"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1717"/>
    <dbReference type="eggNOG" id="COG2175">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036005_2_1_4"/>
    <dbReference type="OMA" id="PPFGCVL"/>
    <dbReference type="OrthoDB" id="1742732at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000908">
        <property type="term" value="F:taurine dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.130.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042098">
        <property type="entry name" value="TauD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003819">
        <property type="entry name" value="TauD/TfdA-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02668">
        <property type="entry name" value="TauD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="1 3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TauD" evidence="2">
        <location>
            <begin position="2"/>
            <end position="273"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF08598.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="282" mass="32082" checksum="D2414A1D73D0D879" modified="2006-05-30" version="1">MQVKPLTCAIGAELVGVNLADAIKSDDLFAEIRAQLLKHRVVFLRDQTFSRAEHVAFARRFGELEDHPVAGSDPEHPGLVRIYKSPDQPNDRYENAWHSDTTWREAPQFGAVLRCVECPPVGGDTMWANMALAYENLPDHIKSQIADLRARHSIEASFGAVMPIEKRLALKERYPDAEHPVVRTHPDTGEKILYVNAFTTHFTNYHTPARVRFGQDGNPGATDLLRYLISQAYIPEYQVRWRWQPNSVAIWDNTATQHYAVMDYPPCYRKMERAGIIGSVPF</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="67" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LMN5</accession>
    <name>Q1LMN5_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Outer membrane heavy metal efflux protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Rmet_1710</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the outer membrane factor (OMF) (TC 1.B.17) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08591.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516443.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1710"/>
    <dbReference type="EnsemblBacteria" id="ABF08591">
        <property type="protein sequence ID" value="ABF08591"/>
        <property type="gene ID" value="Rmet_1710"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1710"/>
    <dbReference type="eggNOG" id="COG1538">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012817_13_0_4"/>
    <dbReference type="OMA" id="DFWGRFS"/>
    <dbReference type="OrthoDB" id="1619477at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015562">
        <property type="term" value="F:efflux transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003423">
        <property type="entry name" value="OMP_efflux"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010131">
        <property type="entry name" value="RND_efflux_OM_lipoprot_NodT"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02321">
        <property type="entry name" value="OEP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01845">
        <property type="entry name" value="outer_NodT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0564">Palmitate</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1134">Transmembrane beta strand</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="477"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="501"/>
            <end position="519"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU362097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF08591.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="519" mass="57438" checksum="A14A2F08B1E4B31A" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="97" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LMY4</accession>
    <name>Q1LMY4_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Transcriptional regulator, GntR family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_1609</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">In the C-terminal section; belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08492.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1609"/>
    <dbReference type="EnsemblBacteria" id="ABF08492">
        <property type="protein sequence ID" value="ABF08492"/>
        <property type="gene ID" value="Rmet_1609"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1609"/>
    <dbReference type="eggNOG" id="COG1167">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017584_0_1_4"/>
    <dbReference type="OMA" id="AFPITNW"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009058">
        <property type="term" value="P:biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07377">
        <property type="entry name" value="WHTH_GntR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000524">
        <property type="entry name" value="Tscrpt_reg_HTH_GntR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00392">
        <property type="entry name" value="GntR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00035">
        <property type="entry name" value="HTHGNTR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00345">
        <property type="entry name" value="HTH_GNTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="SSF53383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50949">
        <property type="entry name" value="HTH_GNTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH gntR-type" evidence="2">
        <location>
            <begin position="65"/>
            <end position="133"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF08492.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="569" mass="62708" checksum="24D90448A6DC22A0" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LN55</accession>
    <name>Q1LN55_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Hydrogenase expression/formation protein HypC</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">hypC2</name>
        <name evidence="2" type="ordered locus">Rmet_1538</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the HupF/HypC family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08421.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1538"/>
    <dbReference type="EnsemblBacteria" id="ABF08421">
        <property type="protein sequence ID" value="ABF08421"/>
        <property type="gene ID" value="Rmet_1538"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1538"/>
    <dbReference type="eggNOG" id="COG0298">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_159381_2_1_4"/>
    <dbReference type="OMA" id="VILHVGH"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001109">
        <property type="entry name" value="Hydrogenase_HupF/HypC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35177">
        <property type="entry name" value="PTHR35177"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01455">
        <property type="entry name" value="HupF_HypC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00445">
        <property type="entry name" value="HUPFHYPC"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00074">
        <property type="entry name" value="hypC_hupF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF08421.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="62" mass="6590" checksum="C11F51DF11C63819" modified="2006-05-30" version="1">MALIDLGGVRKEISLALVDDVAVGDYVIVHVGYALTKLDPEEAERTLKLFAEAGMVQTEGAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LNI1</accession>
    <name>Q1LNI1_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">D-aminopeptidase M55</fullName>
            <ecNumber evidence="3">3.4.11.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">dppA</name>
        <name evidence="3" type="ordered locus">Rmet_1412</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EC" id="3.4.11.-" evidence="3"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08295.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516167.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1412"/>
    <dbReference type="EnsemblBacteria" id="ABF08295">
        <property type="protein sequence ID" value="ABF08295"/>
        <property type="gene ID" value="Rmet_1412"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1412"/>
    <dbReference type="eggNOG" id="COG2362">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_086038_1_1_4"/>
    <dbReference type="OMA" id="ATGVTWP"/>
    <dbReference type="OrthoDB" id="1439650at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004177">
        <property type="term" value="F:aminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10780">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027476">
        <property type="entry name" value="DppA_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007035">
        <property type="entry name" value="Peptidase_M55"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036177">
        <property type="entry name" value="Peptidase_M55_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04951">
        <property type="entry name" value="Peptidase_M55"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015853">
        <property type="entry name" value="Pep_DppA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63992">
        <property type="entry name" value="SSF63992"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0031">Aminopeptidase</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0862">Zinc</keyword>
    <feature type="active site" description="Nucleophile" evidence="1">
        <location>
            <position position="115"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="8"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="10"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="60"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="104"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="134"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRSR" id="PIRSR015853-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR015853-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF08295.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="275" mass="29187" checksum="454D30E5833EA89A" modified="2006-05-30" version="1">MKILVSTDIEGVAGVFHAEQTRAGNGEYERARAWMTAEANAAVQGAFAGGATEVLVNDSHGGFRNLLPDQIDPRAQLVLGKPRYLSMMAGVEQDCDGVFMIGYHGRAQSRGVLAHTINSGAFARVWLNGLELGEAGIYAALAGEFGVPVMLASGDDVFIAETQPLMPWVRYVQTKAAGGQGSGTSLSPAAAREAIAVAAESVTRDAIVQAKARCFELPGPVSCRLQTTTTAHADLFCQWPEFERVDGVTLTFTGPTVQAVVRMLNCCSAMSFMLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="120" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LNL5</accession>
    <name>Q1LNL5_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Sulfate/thiosulfate transporter subunit ATP-binding component of ABC superfamilysulfate/thiosulfate transporter subunit ATP-binding component of ABC superfamily</fullName>
            <ecNumber evidence="11">3.6.3.25</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">cysA</name>
        <name evidence="11" type="ordered locus">Rmet_1378</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EC" id="3.6.3.25" evidence="11"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08261.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516139.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1378"/>
    <dbReference type="EnsemblBacteria" id="ABF08261">
        <property type="protein sequence ID" value="ABF08261"/>
        <property type="gene ID" value="Rmet_1378"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1378"/>
    <dbReference type="eggNOG" id="COG1118">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_1_4"/>
    <dbReference type="OMA" id="HLGWEIQ"/>
    <dbReference type="OrthoDB" id="1220708at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043190">
        <property type="term" value="C:ATP-binding cassette (ABC) transporter complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015419">
        <property type="term" value="F:ABC-type sulfate transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102025">
        <property type="term" value="F:ABC-type thiosulfate transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03296">
        <property type="entry name" value="ABC_CysA_sulfate_importer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041193">
        <property type="entry name" value="CysA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008995">
        <property type="entry name" value="Mo/tungstate-bd_C_term_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005666">
        <property type="entry name" value="Sulph_transpt1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024765">
        <property type="entry name" value="TOBE-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17850">
        <property type="entry name" value="CysA_C_terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12857">
        <property type="entry name" value="TOBE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50331">
        <property type="entry name" value="SSF50331"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00968">
        <property type="entry name" value="3a0106s01"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 9 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0764">Sulfate transport</keyword>
    <keyword evidence="6" id="KW-1278">Translocase</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ABC transporter" evidence="10">
        <location>
            <begin position="3"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="35"/>
            <end position="42"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ABF08261.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="367" mass="40778" checksum="19DC691B1CF82A33" modified="2006-05-30" version="1">MSIQVRNVHKRFGDFVALNDVSLDFAEGELTALLGPSGCGKTTLLRIIAGLERADAGQVVLAGQDASTQHVRQRNVGFVFQHYALFKHMTVFENVAFGLRVKPRGERPSEAKIREKVKSLLELVQLDWLADRFPAQLSGGQRQRIALARALAVEPRVLLLDEPFGALDAKVRKELRRWLRRLHDDLHVTSVFVTHDQEEALEVADQVVLMNRGSVEQFGTPEAVYNHPATPFVFGFLGNVNLFHGRLEVGDSGGLLHTGEAVLPVVGSGHETEGDAVAYVRPHDLDLERYAPGTDGIAVTLRRALTLGPVAQLELEREDNQDVIEVALPLERFRHHGFKEGELLAVRPRQLRVFNQGRTTQEQGAAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LNQ9</accession>
    <name>Q1LNQ9_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Universal stress protein, UspA family (Protein belongs to CMGI-2)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">uspA2</name>
        <name evidence="3" type="ordered locus">Rmet_1334</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal stress protein A family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08217.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516099.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1334"/>
    <dbReference type="EnsemblBacteria" id="ABF08217">
        <property type="protein sequence ID" value="ABF08217"/>
        <property type="gene ID" value="Rmet_1334"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1334"/>
    <dbReference type="eggNOG" id="COG0589">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_049301_11_0_4"/>
    <dbReference type="OMA" id="ANEFNAD"/>
    <dbReference type="OrthoDB" id="2040681at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006015">
        <property type="entry name" value="Universal_stress_UspA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006016">
        <property type="entry name" value="UspA"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00582">
        <property type="entry name" value="Usp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01438">
        <property type="entry name" value="UNVRSLSTRESS"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Usp" evidence="2">
        <location>
            <begin position="1"/>
            <end position="146"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008791"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF08217.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="154" mass="16994" checksum="2282304517B4EE17" modified="2006-05-30" version="1">MYSRFLVPLDGSPTSLLALDHAADMARLNGATVVLLHVIEPTRHSTGFERPKVYIEDVRPHFLAAGHALLDEAANRLRQDGIATETVLLESTAERVSEQIARQAKENDCDLVILGTHGRRGVDRLLLGSDAEQVARIAPVPVMLVRQSRRKEIT</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LP05</accession>
    <name>Q1LP05_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Putative ATP-dependent helicase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">yoaA</name>
        <name evidence="6" type="ordered locus">Rmet_1235</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08121.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1235"/>
    <dbReference type="EnsemblBacteria" id="ABF08121">
        <property type="protein sequence ID" value="ABF08121"/>
        <property type="gene ID" value="Rmet_1235"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1235"/>
    <dbReference type="eggNOG" id="COG1199">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012117_2_0_4"/>
    <dbReference type="OMA" id="VVTNHAM"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016818">
        <property type="term" value="F:hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006139">
        <property type="term" value="P:nucleobase-containing compound metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006555">
        <property type="entry name" value="ATP-dep_Helicase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045028">
        <property type="entry name" value="DinG/Rad3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014013">
        <property type="entry name" value="Helic_SF1/SF2_ATP-bd_DinG/Rad3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11472">
        <property type="entry name" value="PTHR11472"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13307">
        <property type="entry name" value="Helicase_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00491">
        <property type="entry name" value="HELICc2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51193">
        <property type="entry name" value="HELICASE_ATP_BIND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0347">Helicase</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="5">
        <location>
            <begin position="67"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="419"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="7"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABF08121.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="743" mass="81046" checksum="EAE5BC7AF472F811" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LPG2</accession>
    <name>Q1LPG2_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Inositol-1-monophosphatase</fullName>
            <ecNumber evidence="6">3.1.3.25</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">suhB</name>
        <name evidence="7" type="ordered locus">Rmet_1078</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 6">
            <text>a myo-inositol phosphate + H2O = myo-inositol + phosphate</text>
            <dbReference type="Rhea" id="RHEA:24056"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:17268"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:84139"/>
            <dbReference type="EC" id="3.1.3.25"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 6">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3 6">Belongs to the inositol monophosphatase superfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.25" evidence="6"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07964.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515866.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1078"/>
    <dbReference type="EnsemblBacteria" id="ABF07964">
        <property type="protein sequence ID" value="ABF07964"/>
        <property type="gene ID" value="Rmet_1078"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1078"/>
    <dbReference type="eggNOG" id="COG0483">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_044118_0_4_4"/>
    <dbReference type="OMA" id="VHQELEF"/>
    <dbReference type="OrthoDB" id="1741160at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008934">
        <property type="term" value="F:inositol monophosphate 1-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052832">
        <property type="term" value="F:inositol monophosphate 3-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052833">
        <property type="term" value="F:inositol monophosphate 4-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046855">
        <property type="term" value="P:inositol phosphate dephosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046854">
        <property type="term" value="P:phosphatidylinositol phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01639">
        <property type="entry name" value="IMPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033942">
        <property type="entry name" value="IMPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020583">
        <property type="entry name" value="Inositol_monoP_metal-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000760">
        <property type="entry name" value="Inositol_monophosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020550">
        <property type="entry name" value="Inositol_monophosphatase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022337">
        <property type="entry name" value="Inositol_monophosphatase_SuhB"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00459">
        <property type="entry name" value="Inositol_P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00377">
        <property type="entry name" value="IMPHPHTASES"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01959">
        <property type="entry name" value="SBIMPHPHTASE"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00629">
        <property type="entry name" value="IMP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00630">
        <property type="entry name" value="IMP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 6" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU364068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABF07964.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="273" mass="29755" checksum="1CD9305420DC1BE9" modified="2006-05-30" version="1">MHPMLNIAVKAARKAGSIINRASIDVDLVRVSRKQHNDFVTEVDRAAEAAIIDIIHTAYPEHAILAEESGQSWADGEEQSEFTWVIDPLDGTTNFIHGFPQYAVSIGLLHKGVPSQAVVYDPTRDELFTASKGAGAFLNNRRIRVTRRDKLADCLIGTGFPFRDLEGMDEYLEMFALMTRSCAGLRRPGAAALDLAYVACGRLDGFFERGLKPWDMAAGMLLITEAGGLVGNYAGESRQMEQGEVLAGNPKAFAQMVRLLAPFSLDNAKPAAV</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-12-12" modified="2022-08-03" version="104" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LPI5</accession>
    <name>ENO1_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Enolase 1</fullName>
            <ecNumber evidence="1">4.2.1.11</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">2-phospho-D-glycerate hydro-lyase 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">2-phosphoglycerate dehydratase 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">eno1</name>
        <name type="ordered locus">Rmet_1055</name>
    </gene>
    <organism>
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the reversible conversion of 2-phosphoglycerate into phosphoenolpyruvate. It is essential for the degradation of carbohydrates via glycolysis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(2R)-2-phosphoglycerate = H2O + phosphoenolpyruvate</text>
            <dbReference type="Rhea" id="RHEA:10164"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:58289"/>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
            <dbReference type="EC" id="4.2.1.11"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="activity regulation">
        <text evidence="1">The covalent binding to the substrate causes inactivation of the enzyme, and possibly serves as a signal for the export of the protein.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 4/5.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cell surface</location>
        </subcellularLocation>
        <text evidence="1">Fractions of enolase are present in both the cytoplasm and on the cell surface. The export of enolase possibly depends on the covalent binding to the substrate; once secreted, it remains attached to the cell surface.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the enolase family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.11" evidence="1"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07941.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008650102.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q1LPI5"/>
    <dbReference type="SMR" id="Q1LPI5"/>
    <dbReference type="STRING" id="266264.Rmet_1055"/>
    <dbReference type="EnsemblBacteria" id="ABF07941">
        <property type="protein sequence ID" value="ABF07941"/>
        <property type="gene ID" value="Rmet_1055"/>
    </dbReference>
    <dbReference type="GeneID" id="60821916"/>
    <dbReference type="KEGG" id="rme:Rmet_1055"/>
    <dbReference type="eggNOG" id="COG0148">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031223_2_1_4"/>
    <dbReference type="OMA" id="EFMIIPV"/>
    <dbReference type="OrthoDB" id="533698at2"/>
    <dbReference type="UniPathway" id="UPA00109">
        <property type="reaction ID" value="UER00187"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009986">
        <property type="term" value="C:cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000015">
        <property type="term" value="C:phosphopyruvate hydratase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004634">
        <property type="term" value="F:phosphopyruvate hydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006096">
        <property type="term" value="P:glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd03313">
        <property type="entry name" value="enolase"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Enolase"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Enolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036849">
        <property type="entry name" value="Enolase-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029017">
        <property type="entry name" value="Enolase-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020810">
        <property type="entry name" value="Enolase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020809">
        <property type="entry name" value="Enolase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020811">
        <property type="entry name" value="Enolase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11902">
        <property type="entry name" value="PTHR11902"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00113">
        <property type="entry name" value="Enolase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03952">
        <property type="entry name" value="Enolase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001400">
        <property type="entry name" value="Enolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00148">
        <property type="entry name" value="ENOLASE"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00002">
        <property type="entry name" value="enolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01192">
        <property type="entry name" value="Enolase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01193">
        <property type="entry name" value="Enolase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51604">
        <property type="entry name" value="SSF51604"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54826">
        <property type="entry name" value="SSF54826"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01060">
        <property type="entry name" value="eno"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00164">
        <property type="entry name" value="ENOLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0324">Glycolysis</keyword>
    <keyword id="KW-0456">Lyase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0964">Secreted</keyword>
    <feature type="chain" id="PRO_0000267084" description="Enolase 1">
        <location>
            <begin position="1"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="1">
        <location>
            <position position="205"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="1">
        <location>
            <position position="339"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="155"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="164"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="242"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="287"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="287"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="314"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="314"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" description="covalent; in inhibited form" evidence="1">
        <location>
            <position position="339"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="366"/>
            <end position="369"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="390"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00318"/>
        </source>
    </evidence>
    <sequence length="429" mass="45938" checksum="A5E3C4079BE94473" modified="2006-05-30" version="1">MSAIVDIIGREVLDSRGNPTVECDVLLESGVMGRAAVPSGASTGSREAIELRDGDKSRYLGKGVLKAVEHINTEISEAIMGLDASEQAFLDRTLIELDGTENKGRLGANAMLAVSMAVAKAAAEEAGLPLYRYFGGSGAMQMPVPMMNIVNGGAHANNSLDIQEFMIMPVSQTSFREALRCGAEVFHALKKILSDKGMSTAVGDEGGFAPNFSSNEECLNTVVQAIEKAGYKAGEDVLLALDCAASEFYHEAEGVYSLEGEGLKLTSTQFADYLANLCDKFPIVSIEDGMAEGDWEGWKTLTDKLGKRVQLVGDDLFVTNTKILKEGIEKGIGNSILIKINQIGTLTETFAAIEMAKRAGYTAVISHRSGETEDSTIADIAVGTNAGQIKTGSLSRSDRISKYNQLLRIEEDLGDIATYPGKSTFYNLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LPJ0</accession>
    <name>Q1LPJ0_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_1050</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07936.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1050"/>
    <dbReference type="EnsemblBacteria" id="ABF07936">
        <property type="protein sequence ID" value="ABF07936"/>
        <property type="gene ID" value="Rmet_1050"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1050"/>
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        <property type="taxonomic scope" value="Bacteria"/>
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    <dbReference type="HOGENOM" id="CLU_066183_0_0_4"/>
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        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016545">
        <property type="entry name" value="UCP009120_prtse"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF009120">
        <property type="entry name" value="UCP009120_prtse"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="SSF56235"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="244"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="249"/>
            <end position="268"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF07936.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="268" mass="30010" checksum="EDF431C7A4E17FB9" modified="2006-05-30" version="1">MTYCVAMRLDAGLVFLSDSRTNAGVDAISTARKMTVFEEPGDRVMVLLTSGNLAISQSVRQCLAEARDEKRSLWTARDMFEAVTIVGEAVRAVHKRDAHALREAGIEFSVNLIFGGQIRGERMRLFHVYAAGNFIEATPENCYFQIGEAKYGKPIVDRVITPSVPLGEAAKCALVSMDSTLKSNISVGLPLDLLVYEADSLRVTRFVSIDEKNAYFRMIRDTWGQRLRQVFGEIANPEWDASQQAEFPLARDEADRWGQPVRARRDRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LPN2</accession>
    <name>Q1LPN2_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_1008</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
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        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07894.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515808.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1008"/>
    <dbReference type="EnsemblBacteria" id="ABF07894">
        <property type="protein sequence ID" value="ABF07894"/>
        <property type="gene ID" value="Rmet_1008"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1008"/>
    <dbReference type="eggNOG" id="COG0697">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033863_9_3_4"/>
    <dbReference type="OMA" id="PFQIMAN"/>
    <dbReference type="OrthoDB" id="1515911at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000620">
        <property type="entry name" value="EamA_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00892">
        <property type="entry name" value="EamA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="16"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="46"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="78"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="106"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="134"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="158"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="183"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="224"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="domain" description="EamA" evidence="2">
        <location>
            <begin position="17"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="domain" description="EamA" evidence="2">
        <location>
            <begin position="158"/>
            <end position="296"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF07894.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="312" mass="31582" checksum="2722AE387C6E59DF" modified="2006-05-30" version="1">MTASATGTLGHARDKLAGVLLIALSASAFGAMAIFARFAYAAGADVYGLLAVRFLLAAIAMGFVMRARGISLPPWRRVLALAAMGGIGYVSQSYCFFTALNYAQASLVALLLYLYPLFVTILAAVFLKEHLTTSTVIALVLCSVGAGLTVGGGEGSSLGIALGVAAAVIYSIYIVVGARVTAGVNAIATTTVICTAAALVYVTLGLLRAGAGVPPQFPASAGGWLALVAIALVSTVLAILTFFAGLQRLGASKASMLSTLEPVVTVVLAAVLLGEHIGPTQAVGGGLILAGVLWLTRRASGTPPLKGSDERN</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="113" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LPP6</accession>
    <name>Q1LPP6_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Bifunctional glutamine synthetase adenylyltransferase/adenylyl-removing enzyme</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">ATP:glutamine synthetase adenylyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">ATase</fullName>
        </alternativeName>
        <domain>
            <recommendedName>
                <fullName evidence="6">Glutamine synthetase adenylyl-L-tyrosine phosphorylase</fullName>
                <ecNumber evidence="6">2.7.7.89</ecNumber>
            </recommendedName>
            <alternativeName>
                <fullName evidence="6">Adenylyl removase</fullName>
                <shortName evidence="6">AR</shortName>
                <shortName evidence="6">AT-N</shortName>
            </alternativeName>
        </domain>
        <domain>
            <recommendedName>
                <fullName evidence="6">Glutamine synthetase adenylyl transferase</fullName>
                <ecNumber evidence="6">2.7.7.42</ecNumber>
            </recommendedName>
            <alternativeName>
                <fullName evidence="6">Adenylyl transferase</fullName>
                <shortName evidence="6">AT</shortName>
                <shortName evidence="6">AT-C</shortName>
            </alternativeName>
        </domain>
    </protein>
    <gene>
        <name evidence="6 10" type="primary">glnE</name>
        <name evidence="10" type="ordered locus">Rmet_0994</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Involved in the regulation of glutamine synthetase GlnA, a key enzyme in the process to assimilate ammonia. When cellular nitrogen levels are high, the C-terminal adenylyl transferase (AT) inactivates GlnA by covalent transfer of an adenylyl group from ATP to specific tyrosine residue of GlnA, thus reducing its activity. Conversely, when nitrogen levels are low, the N-terminal adenylyl removase (AR) activates GlnA by removing the adenylyl group by phosphorolysis, increasing its activity. The regulatory region of GlnE binds the signal transduction protein PII (GlnB) which indicates the nitrogen status of the cell.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>[glutamine synthetase]-L-tyrosine + ATP = [glutamine synthetase]-O(4)-(5'-adenylyl)-L-tyrosine + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:18589"/>
            <dbReference type="Rhea" id="RHEA-COMP:10660"/>
            <dbReference type="Rhea" id="RHEA-COMP:10661"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:83624"/>
            <dbReference type="EC" id="2.7.7.42"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>[glutamine synthetase]-O(4)-(5'-adenylyl)-L-tyrosine + phosphate = [glutamine synthetase]-L-tyrosine + ADP</text>
            <dbReference type="Rhea" id="RHEA:43716"/>
            <dbReference type="Rhea" id="RHEA-COMP:10660"/>
            <dbReference type="Rhea" id="RHEA-COMP:10661"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:83624"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.7.89"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the GlnE family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.89" evidence="6"/>
    <dbReference type="EC" id="2.7.7.42" evidence="6"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07880.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0994"/>
    <dbReference type="EnsemblBacteria" id="ABF07880">
        <property type="protein sequence ID" value="ABF07880"/>
        <property type="gene ID" value="Rmet_0994"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0994"/>
    <dbReference type="eggNOG" id="COG1391">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006233_0_1_4"/>
    <dbReference type="OMA" id="EFMVQYA"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008882">
        <property type="term" value="F:[glutamate-ammonia-ligase] adenylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047388">
        <property type="term" value="F:[glutamine synthetase]-adenylyl-L-tyrosine phosphorylase"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008152">
        <property type="term" value="P:metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000820">
        <property type="term" value="P:regulation of glutamine family amino acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.460.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00802">
        <property type="entry name" value="GlnE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023057">
        <property type="entry name" value="GlnE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005190">
        <property type="entry name" value="GlnE_rpt_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043519">
        <property type="entry name" value="NT_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013546">
        <property type="entry name" value="PII_UdlTrfase/GS_AdlTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30621">
        <property type="entry name" value="PTHR30621"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08335">
        <property type="entry name" value="GlnD_UR_UTase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03710">
        <property type="entry name" value="GlnE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81301">
        <property type="entry name" value="SSF81301"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 6" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5 6" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="2 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="1 6 10" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 10" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="GlnE" evidence="8">
        <location>
            <begin position="86"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="domain" description="GlnD_UR_UTase" evidence="9">
        <location>
            <begin position="355"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="domain" description="GlnE" evidence="8">
        <location>
            <begin position="634"/>
            <end position="870"/>
        </location>
    </feature>
    <feature type="domain" description="GlnD_UR_UTase" evidence="9">
        <location>
            <begin position="892"/>
            <end position="981"/>
        </location>
    </feature>
    <feature type="region of interest" description="Adenylyl removase" evidence="6">
        <location>
            <begin position="1"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="17"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="region of interest" description="Adenylyl transferase" evidence="6">
        <location>
            <begin position="516"/>
            <end position="1012"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="522"/>
            <end position="548"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="18"/>
            <end position="49"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00802"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF03710"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF08335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ABF07880.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="1012" mass="111556" checksum="F5EBF97A46CF4F86" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LPQ3</accession>
    <name>Q1LPQ3_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Cytochrome c, class IC 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Rmet_0987</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07873.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008641741.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0987"/>
    <dbReference type="EnsemblBacteria" id="ABF07873">
        <property type="protein sequence ID" value="ABF07873"/>
        <property type="gene ID" value="Rmet_0987"/>
    </dbReference>
    <dbReference type="GeneID" id="60821829"/>
    <dbReference type="KEGG" id="rme:Rmet_0987"/>
    <dbReference type="eggNOG" id="COG2863">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_128253_1_2_4"/>
    <dbReference type="OMA" id="KVAMCIG"/>
    <dbReference type="OrthoDB" id="1939800at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.760.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009056">
        <property type="entry name" value="Cyt_c-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036909">
        <property type="entry name" value="Cyt_c-like_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46626">
        <property type="entry name" value="SSF46626"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51007">
        <property type="entry name" value="CYTC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 4" id="KW-0349">Heme</keyword>
    <keyword evidence="3 4" id="KW-0408">Iron</keyword>
    <keyword evidence="2 4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193684" evidence="5">
        <location>
            <begin position="20"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="domain" description="Cytochrome c" evidence="6">
        <location>
            <begin position="23"/>
            <end position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABF07873.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="114" mass="12290" checksum="3EF3E8A356B710ED" modified="2006-05-30" version="1">MKKLLTMVVLGAAATAAQAQDKGNVDAAKDKVAMCIGCHGIPGYRASFPEVYQVPMLGGQSAKYIENALKSYQKGDRKHPTMRGIADSLSDKDIAAVAAYYSQQKRDTQNNPLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="68" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LPR0</accession>
    <name>Q1LPR0_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_0980</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
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            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
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            <end position="18"/>
        </location>
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        </location>
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            <end position="243"/>
        </location>
    </feature>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="ABF07866.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="250" mass="25571" checksum="EA426E6CD04E8B81" modified="2006-05-30" version="1">MLSAAVAGAIALAGCATAPTDETTAVAGQPGSPAASTSTPVAPGPASATAAKTPTANGKSPPTTTVTLPDRGRVSLAEYRAQMRDQLMKTENASADVADCAAHASWVVPRSATYDALKIPTGALTQDQATEERWESRFSSGKQSVVVSSVVTFAASAHRRSGEPQWQPVKVRCGYDNGMMLAYELLNSSGETISEPAAAPAVATSHSKKKKGKSTAKASTSSKSSKASSTSKSTSSSKSTSSKTTTKKKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-12-02" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LPZ2</accession>
    <name>Q1LPZ2_CUPMC</name>
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    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
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                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
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            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
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    </dbReference>
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    </dbReference>
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            <begin position="28"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
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            <end position="836"/>
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        <source>
            <dbReference type="EMBL" id="ABF07784.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="851" mass="91628" checksum="642D104E9C99A5F7" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="80" xmlns="http://uniprot.org/uniprot">
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        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
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                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07755.1"/>
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    </dbReference>
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    <dbReference type="EnsemblBacteria" id="ABF07755">
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    </dbReference>
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    </dbReference>
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        <location>
            <begin position="216"/>
            <end position="270"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01593"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF07755.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="539" mass="58582" checksum="877545339682AC2F" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LQ46</accession>
    <name>Q1LQ46_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Putative Acyl-CoA dehydrogenase</fullName>
            <ecNumber evidence="8">1.3.8.1</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Rmet_0844</name>
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        <name type="synonym">Ralstonia metallidurans</name>
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            <taxon>Bacteria</taxon>
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            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
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        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
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        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the acyl-CoA dehydrogenase family.</text>
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        <property type="protein sequence ID" value="ABF07730.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
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    <dbReference type="EnsemblBacteria" id="ABF07730">
        <property type="protein sequence ID" value="ABF07730"/>
        <property type="gene ID" value="Rmet_0844"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0844"/>
    <dbReference type="eggNOG" id="COG1960">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
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        <property type="component" value="Chromosome"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom_sf"/>
    </dbReference>
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        <property type="entry name" value="AcylCo_DH-like_C"/>
    </dbReference>
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        <property type="entry name" value="AcylCo_DH/oxidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013786">
        <property type="entry name" value="AcylCoA_DH/ox_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037069">
        <property type="entry name" value="AcylCoA_DH/ox_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009100">
        <property type="entry name" value="AcylCoA_DH/oxidase_NM_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00441">
        <property type="entry name" value="Acyl-CoA_dh_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02770">
        <property type="entry name" value="Acyl-CoA_dh_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02771">
        <property type="entry name" value="Acyl-CoA_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47203">
        <property type="entry name" value="SSF47203"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56645">
        <property type="entry name" value="SSF56645"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0274">FAD</keyword>
    <keyword evidence="3" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Acyl-CoA_dh_N" evidence="7">
        <location>
            <begin position="24"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA_dh_M" evidence="6">
        <location>
            <begin position="116"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA_dh_1" evidence="5">
        <location>
            <begin position="240"/>
            <end position="369"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF02770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF02771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ABF07730.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="402" mass="42969" checksum="4A60A9E091955C69" modified="2006-05-30" version="1">MQLTLTETQTLLESSALHWLAGNADDIRQARDARAYWPAFAEMGWLALPFADADGGFGGGPIEVGLLMRAFGRYRVAAPGYRSGVLMAGKLIAALGSMQQRDTWLAPLITGERCLAMAHTEPGREDPWAMPATTATWDPRGERWTLRGKKSLVTGGADADALVISASIHAEGEHPRIGLFIVPSDADGMTSRACLVADGSRAADLVLDEVYLPDDARLGQNAPEQEIAEGFMTIVAEALVASCWEAAGAMRAALEATVSHVTQREQFGRPLSALQSVQHGLAEMAVCCEEAGAACELAALRIAADRRLARDAASLAQSCTGRAARYVAAQAVQLHGGMGVSEELHVAALFRLLTRFRLQDGHADWHSMQLGNDMYTGDWRMSQTLLDPATSGQAVAAYAEAH</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="104" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LQC9</accession>
    <name>Q1LQC9_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Carbon-phosphorus lyase complex subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">phnL</name>
        <name evidence="9" type="ordered locus">Rmet_0761</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07647.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0761"/>
    <dbReference type="EnsemblBacteria" id="ABF07647">
        <property type="protein sequence ID" value="ABF07647"/>
        <property type="gene ID" value="Rmet_0761"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0761"/>
    <dbReference type="eggNOG" id="COG4778">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_22_4"/>
    <dbReference type="OMA" id="MHLRDGI"/>
    <dbReference type="OrthoDB" id="1181903at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012701">
        <property type="entry name" value="CP_lyase_PhnL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02324">
        <property type="entry name" value="CP_lyasePhnL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="1" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-0456">Lyase</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-1278">Translocase</keyword>
    <feature type="domain" description="ABC transporter" evidence="8">
        <location>
            <begin position="10"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="49"/>
            <end position="56"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ABF07647.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="241" mass="26455" checksum="91A03BD40E353E0B" modified="2006-05-30" version="1">MMQANNQKLIEVRGLGKMFTLHNQGGIRLPVLRAIDFDASRGECLVLSGRSGTGKSTLLRCLYGNYLATEGTIRLRDTSEASEPWVELTNAPEQRVLKLRRDVIGYVSQFLRAIPRVSALDVVADPLQQRGASHEEARARAATLLERLNLPRRLWDLPPATFSGGEQQRVNIARGLIGGHPILLLDEPTASLDADNRAVVIELIREALAEGRSLIGIFHDEAVRDAVATRMLPLQPATALA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="112" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LQI7</accession>
    <name>Q1LQI7_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Cyanophycin synthetase</fullName>
            <ecNumber evidence="7">6.3.2.29</ecNumber>
            <ecNumber evidence="6">6.3.2.30</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Cyanophycin synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="primary">cphA1</name>
        <name evidence="12" type="ordered locus">Rmet_0703</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Catalyzes the ATP-dependent polymerization of arginine and aspartate to multi-L-arginyl-poly-L-aspartic acid (cyanophycin; a water-insoluble reserve polymer).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>[L-4-(L-arginin-2-N-yl)aspartate](n) + ATP + L-aspartate = [L-4-(L-arginin-2-N-yl)aspartate](n)-L-aspartate + ADP + H(+) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:13277"/>
            <dbReference type="Rhea" id="RHEA-COMP:13728"/>
            <dbReference type="Rhea" id="RHEA-COMP:13733"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:137986"/>
            <dbReference type="ChEBI" id="CHEBI:137990"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.2.29"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>[L-4-(L-arginin-2-N-yl)aspartate](n)-L-aspartate + ATP + L-arginine = [L-4-(L-arginin-2-N-yl)aspartate](n+1) + ADP + H(+) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:23888"/>
            <dbReference type="Rhea" id="RHEA-COMP:13732"/>
            <dbReference type="Rhea" id="RHEA-COMP:13733"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:32682"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:137986"/>
            <dbReference type="ChEBI" id="CHEBI:137990"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.2.30"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="5">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">In the C-terminal section; belongs to the MurCDEF family.</text>
    </comment>
    <dbReference type="EC" id="6.3.2.29" evidence="7"/>
    <dbReference type="EC" id="6.3.2.30" evidence="6"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07589.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515553.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0703"/>
    <dbReference type="EnsemblBacteria" id="ABF07589">
        <property type="protein sequence ID" value="ABF07589"/>
        <property type="gene ID" value="Rmet_0703"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0703"/>
    <dbReference type="eggNOG" id="COG0189">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG0769">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016806_0_0_4"/>
    <dbReference type="OMA" id="PFMIANA"/>
    <dbReference type="OrthoDB" id="1861122at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071161">
        <property type="term" value="F:cyanophycin synthetase activity (L-arginine-adding)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071160">
        <property type="term" value="F:cyanophycin synthetase activity (L-aspartate-adding)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004326">
        <property type="term" value="F:tetrahydrofolylpolyglutamate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009059">
        <property type="term" value="P:macromolecule biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1190.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011761">
        <property type="entry name" value="ATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005479">
        <property type="entry name" value="CbamoylP_synth_lsu-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011810">
        <property type="entry name" value="Cya_phycin_syn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044019">
        <property type="entry name" value="Cyanophycin_syn_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018109">
        <property type="entry name" value="Folylpolyglutamate_synth_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036565">
        <property type="entry name" value="Mur-like_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004101">
        <property type="entry name" value="Mur_ligase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036615">
        <property type="entry name" value="Mur_ligase_C_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013221">
        <property type="entry name" value="Mur_ligase_cen"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02786">
        <property type="entry name" value="CPSase_L_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18921">
        <property type="entry name" value="Cyanophycin_syn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02875">
        <property type="entry name" value="Mur_ligase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08245">
        <property type="entry name" value="Mur_ligase_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53244">
        <property type="entry name" value="SSF53244"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53623">
        <property type="entry name" value="SSF53623"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02068">
        <property type="entry name" value="cya_phycin_syn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50975">
        <property type="entry name" value="ATP_GRASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01011">
        <property type="entry name" value="FOLYLPOLYGLU_SYNT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="12" id="KW-0436">Ligase</keyword>
    <keyword evidence="10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ATP-grasp" evidence="11">
        <location>
            <begin position="209"/>
            <end position="462"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00000917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00003184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009060"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00013005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00031353"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ABF07589.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="856" mass="91431" checksum="2BABF4F926CAEA5A" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="97" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LQL3</accession>
    <name>Q1LQL3_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Gamma-Glu-gamma-aminobutyraldehyde dehydrogenase, NAD(P)H-dependent</fullName>
            <ecNumber evidence="6">1.2.1.3</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">puuC</name>
        <name evidence="6" type="ordered locus">Rmet_0677</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the aldehyde dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.2.1.3" evidence="6"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07563.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515532.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0677"/>
    <dbReference type="EnsemblBacteria" id="ABF07563">
        <property type="protein sequence ID" value="ABF07563"/>
        <property type="gene ID" value="Rmet_0677"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0677"/>
    <dbReference type="eggNOG" id="COG1012">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005391_0_2_4"/>
    <dbReference type="OMA" id="VTGPGHT"/>
    <dbReference type="OrthoDB" id="744602at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004029">
        <property type="term" value="F:aldehyde dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043878">
        <property type="term" value="F:glyceraldehyde-3-phosphate dehydrogenase (NAD+) (non-phosphorylating) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.309.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.605.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016161">
        <property type="entry name" value="Ald_DH/histidinol_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016163">
        <property type="entry name" value="Ald_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016160">
        <property type="entry name" value="Ald_DH_CS_CYS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029510">
        <property type="entry name" value="Ald_DH_CS_GLU"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016162">
        <property type="entry name" value="Ald_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015590">
        <property type="entry name" value="Aldehyde_DH_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00171">
        <property type="entry name" value="Aldedh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53720">
        <property type="entry name" value="SSF53720"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00070">
        <property type="entry name" value="ALDEHYDE_DEHYDR_CYS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00687">
        <property type="entry name" value="ALDEHYDE_DEHYDR_GLU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 4 6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aldedh" evidence="5">
        <location>
            <begin position="18"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="active site" evidence="2">
        <location>
            <position position="247"/>
        </location>
    </feature>
    <feature type="active site" evidence="3">
        <location>
            <position position="281"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABF07563.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="478" mass="51034" checksum="D1547C5C84CF007B" modified="2006-05-30" version="1">MHAQHFVANRLIAPDSGLRIKVFDPSSGQPFAEIARGNATDINVAVHAARRAFDGAWGAMSPADRVRLLNRVALTLIEHEDELAALEARDTGKPLRQARADARAVARYFEFYAGAADKLHGQTIPYQAGYTVLTVREPHGVTGHIIPWNYPLQIFGRSVGAALAAGNACVVKPAEDACLSLLRVAELAAEAGLPEGALNIVTGYGHEAGAALARHPDINHISFTGSPETGKLVSQLAAENHVPVTLELGGKSPQIVFADADLDALLPVVVNGIVQNAGQTCSAGSRLLVERPVYEELLDRVASVFESLRVGPSELDLECGPLISRKQQQRVWDFVSDAQHAGISVMAQGQIVAEAPESGYYQVPMLFRDVPATHRLAQEEVFGPLLAAMPFDTEDEALQLANGTAYGLVAGVWTRDGGRQLRLARKLRAGQVFINNYGAGGGVELPFGGTGQSGYGREKGFEALYGFTTLKTIAIQHG</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="57" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LQP4</accession>
    <name>Q1LQP4_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_0646</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07532.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515503.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0646"/>
    <dbReference type="EnsemblBacteria" id="ABF07532">
        <property type="protein sequence ID" value="ABF07532"/>
        <property type="gene ID" value="Rmet_0646"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0646"/>
    <dbReference type="eggNOG" id="ENOG50339RD">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_170147_0_0_4"/>
    <dbReference type="OMA" id="PSMTIDF"/>
    <dbReference type="OrthoDB" id="1988635at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193358" evidence="1">
        <location>
            <begin position="23"/>
            <end position="115"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF07532.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="115" mass="11568" checksum="20AB659F0CFC8794" modified="2006-05-30" version="1">MNKTLKLSRGATVLAVGTLACIGLSGCMTSTPSWDQHRGEALAAVKQQQIINPDAPAGLPTMDGTDGKTAVAAMRNFDRSLIRVQTGTGSPVGGFGIDFGAGGYGGVGMGGGSQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="95" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LQS0</accession>
    <name>Q1LQS0_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Putative OmpA/MotB (Family of outer membrane porins)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Rmet_0620</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07506.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515477.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0620"/>
    <dbReference type="EnsemblBacteria" id="ABF07506">
        <property type="protein sequence ID" value="ABF07506"/>
        <property type="gene ID" value="Rmet_0620"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0620"/>
    <dbReference type="eggNOG" id="COG2885">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035799_1_0_4"/>
    <dbReference type="OMA" id="GADEWIG"/>
    <dbReference type="OrthoDB" id="1074882at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd07185">
        <property type="entry name" value="OmpA_C-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006664">
        <property type="entry name" value="OMP_bac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006665">
        <property type="entry name" value="OmpA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036737">
        <property type="entry name" value="OmpA-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00691">
        <property type="entry name" value="OmpA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01021">
        <property type="entry name" value="OMPADOMAIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103088">
        <property type="entry name" value="SSF103088"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51123">
        <property type="entry name" value="OMPA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2 3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="34"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="339"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="domain" description="OmpA-like" evidence="4">
        <location>
            <begin position="435"/>
            <end position="553"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABF07506.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="572" mass="61068" checksum="F31F981D5ACCE1B7" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-12-02" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LQW6</accession>
    <name>Q1LQW6_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Fimbrial biogenesis outer membrane usher</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">htrE</name>
        <name evidence="13" type="ordered locus">Rmet_0574</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell outer membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the fimbrial export usher family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07460.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0574"/>
    <dbReference type="EnsemblBacteria" id="ABF07460">
        <property type="protein sequence ID" value="ABF07460"/>
        <property type="gene ID" value="Rmet_0574"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0574"/>
    <dbReference type="eggNOG" id="COG3188">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009120_3_1_4"/>
    <dbReference type="OMA" id="YVQRDIT"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009279">
        <property type="term" value="C:cell outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015473">
        <property type="term" value="F:fimbrial usher porin activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009297">
        <property type="term" value="P:pilus assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.2070">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.2610">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.410">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000015">
        <property type="entry name" value="Fimb_usher"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042186">
        <property type="entry name" value="FimD_plug_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025949">
        <property type="entry name" value="PapC-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043142">
        <property type="entry name" value="PapC-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025885">
        <property type="entry name" value="PapC_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037224">
        <property type="entry name" value="PapC_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30451">
        <property type="entry name" value="PTHR30451"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13953">
        <property type="entry name" value="PapC_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13954">
        <property type="entry name" value="PapC_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00577">
        <property type="entry name" value="Usher"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF141729">
        <property type="entry name" value="SSF141729"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0998">Cell outer membrane</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 10" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1134">Transmembrane beta strand</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193643" evidence="10">
        <location>
            <begin position="29"/>
            <end position="852"/>
        </location>
    </feature>
    <feature type="domain" description="PapC_N" evidence="12">
        <location>
            <begin position="41"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="domain" description="PapC_C" evidence="11">
        <location>
            <begin position="765"/>
            <end position="829"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004571"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF13953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF13954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ABF07460.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="852" mass="91241" checksum="218BAFE711DE7CD5" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LR44</accession>
    <name>Q1LR44_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Major facilitator superfamily (MFS_1) transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Rmet_0496</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07382.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008652156.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0496"/>
    <dbReference type="EnsemblBacteria" id="ABF07382">
        <property type="protein sequence ID" value="ABF07382"/>
        <property type="gene ID" value="Rmet_0496"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0496"/>
    <dbReference type="eggNOG" id="COG2814">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001265_46_6_4"/>
    <dbReference type="OMA" id="LATWVPT"/>
    <dbReference type="OrthoDB" id="1121832at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="SSF103473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="20"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="50"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="84"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="113"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="143"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="170"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="227"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="260"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="292"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="317"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="352"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="380"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="domain" description="MFS" evidence="7">
        <location>
            <begin position="15"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="413"/>
            <end position="441"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ABF07382.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="441" mass="47161" checksum="D211983D50D34CF5" modified="2006-05-30" version="1">MLDWFRELSRAERKGFYGAFLGHAVDVFDFMIYSFLISTLLAQWGMSKSMAGAIVTWTLVSSLVGAIGAGLLADRFGRVRVLRWTILVFAVSCFLCGISTSPEQLMMFRMLQGLGFGGESSLCMVLVTELIRNPAHRGKYSGFTASSYSFGWGGAAIAYAITFNLFEPEVAWRVCFFLGILPALVVVYLRRNLQEPEVFLKSRAERGNVSAAADLARVFRGPLLRKTVLCSLLSGGMLGAYYAIATWLPTFLKTERGLSVFGTSSYLAVTILGSLVGYVAGAYATDRWGRRLTYMAFAAGAFVMAMIYMVIPVSNTSMLFLGFPLGVLMQGVFSGIGATISESYPSSIRATGYGVSYNLGRVIGSFFPLAVGWLNSGRTTLALAIAMVAGVGYALVMVSAAMLPETSGIDLGQAGGGDDGEAKQQAAAEPVTVAPTAGRPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LR88</accession>
    <name>Q1LR88_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Putative enzyme with nucleoside triphosphate hydrolase domain</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">phoL</name>
        <name evidence="5" type="ordered locus">Rmet_0452</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PhoH family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07338.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515326.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0452"/>
    <dbReference type="EnsemblBacteria" id="ABF07338">
        <property type="protein sequence ID" value="ABF07338"/>
        <property type="gene ID" value="Rmet_0452"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0452"/>
    <dbReference type="eggNOG" id="COG1702">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_051654_0_0_4"/>
    <dbReference type="OMA" id="NLCGQFD"/>
    <dbReference type="OrthoDB" id="445854at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003714">
        <property type="entry name" value="PhoH"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02562">
        <property type="entry name" value="PhoH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PhoH" evidence="4">
        <location>
            <begin position="120"/>
            <end position="322"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010393"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF02562"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABF07338.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="331" mass="36943" checksum="CAC2481EA89EE4F3" modified="2006-05-30" version="1">MKIPTAEFVAPRDDNTRLQNLCGPLDENLRQIEQALDVTIQRRGHRMTVRGERAQDATLALERFYNNARVALSIDDVQLGLVESRQVAAHGAYLPSTEGNDGSSIDDESPVLHTRRTGLQGRTAMQRDYLRNILSHDLTLGVGPAGTGKTYLAVACAVDALERDVVKRIVLTRPAVEAGERLGFLPGDLAQKVDPYLRPLYDALYDLLGFDRTQKMFERQMIEIAPLAYMRGRTLNHAFIILDEAQNTTPEQMKMFLTRIGFGSKAVITGDTTQIDLPRGQKSGLVEAQHVLRDVRGVSLTRFTSVDVVRHPLVARIVEAYDEYHAQHKDA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-09-19" modified="2022-08-03" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LRC5</accession>
    <name>ANMK_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Anhydro-N-acetylmuramic acid kinase</fullName>
            <ecNumber evidence="1">2.7.1.170</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">AnhMurNAc kinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">anmK</name>
        <name type="ordered locus">Rmet_0415</name>
    </gene>
    <organism>
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the specific phosphorylation of 1,6-anhydro-N-acetylmuramic acid (anhMurNAc) with the simultaneous cleavage of the 1,6-anhydro ring, generating MurNAc-6-P. Is required for the utilization of anhMurNAc either imported from the medium or derived from its own cell wall murein, and thus plays a role in cell wall recycling.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>1,6-anhydro-N-acetyl-beta-muramate + ATP + H2O = ADP + H(+) + N-acetyl-D-muramate 6-phosphate</text>
            <dbReference type="Rhea" id="RHEA:24952"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58690"/>
            <dbReference type="ChEBI" id="CHEBI:58722"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.170"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-sugar metabolism; 1,6-anhydro-N-acetylmuramate degradation.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cell wall biogenesis; peptidoglycan recycling.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the anhydro-N-acetylmuramic acid kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.170" evidence="1"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07301.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515290.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q1LRC5"/>
    <dbReference type="SMR" id="Q1LRC5"/>
    <dbReference type="STRING" id="266264.Rmet_0415"/>
    <dbReference type="EnsemblBacteria" id="ABF07301">
        <property type="protein sequence ID" value="ABF07301"/>
        <property type="gene ID" value="Rmet_0415"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0415"/>
    <dbReference type="eggNOG" id="COG2377">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038782_0_0_4"/>
    <dbReference type="OMA" id="GQTIRHE"/>
    <dbReference type="OrthoDB" id="736294at2"/>
    <dbReference type="UniPathway" id="UPA00343"/>
    <dbReference type="UniPathway" id="UPA00544"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016773">
        <property type="term" value="F:phosphotransferase activity, alcohol group as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097175">
        <property type="term" value="P:1,6-anhydro-N-acetyl-beta-muramic acid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006040">
        <property type="term" value="P:amino sugar metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009254">
        <property type="term" value="P:peptidoglycan turnover"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01270">
        <property type="entry name" value="AnhMurNAc_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005338">
        <property type="entry name" value="Anhydro_N_Ac-Mur_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30605">
        <property type="entry name" value="PTHR30605"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03702">
        <property type="entry name" value="AnmK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="SSF53067"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword id="KW-0418">Kinase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000250038" description="Anhydro-N-acetylmuramic acid kinase">
        <location>
            <begin position="1"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="17"/>
            <end position="24"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01270"/>
        </source>
    </evidence>
    <sequence length="380" mass="39633" checksum="A4B5292064D0EBA7" modified="2006-05-30" version="1">MPASPQSSEMFIGLMSGTSMDGADGVLVDFSGAQPAVLAAAHTPFPAPLREAFGALQQPGDDEIHREALASNGLARVYADCVATLLRTASVTADRVAAIGAHGQTIRHRPGQYDGTGYTRQSQHPALLAELTGIDVVADFRSRDVAAGGQGAPLVPAVHAALFGAADETRVVCNIGGISNISILPAAGTGRPVTGFDCGPGNALLDDWIHRHRGTPYDDGGAWAASGKVDESLLDQMLAEPYFRALPPKSTGRDLFHPGWLQSLLDTYGNAQPQPVDIQATLATLTATAIAQDVQRYAPEARRLIVCGGGAHNDFVMTTMANQLPGVLVQTTGDFGVPVSQVEAIAFAWLARQCTRRAPGNVATVTGAAGPRVLGAIYPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LRM3</accession>
    <name>Q1LRM3_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Tyrosine-based site-specific recombinase CMGI-7</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">int</name>
        <name evidence="4" type="ordered locus">Rmet_0317</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07203.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515203.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0317"/>
    <dbReference type="EnsemblBacteria" id="ABF07203">
        <property type="protein sequence ID" value="ABF07203"/>
        <property type="gene ID" value="Rmet_0317"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0317"/>
    <dbReference type="eggNOG" id="COG0582">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_048231_3_1_4"/>
    <dbReference type="OMA" id="GERYCHV"/>
    <dbReference type="OrthoDB" id="745068at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.443.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011010">
        <property type="entry name" value="DNA_brk_join_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013762">
        <property type="entry name" value="Integrase-like_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002104">
        <property type="entry name" value="Integrase_catalytic"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00589">
        <property type="entry name" value="Phage_integrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56349">
        <property type="entry name" value="SSF56349"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51898">
        <property type="entry name" value="TYR_RECOMBINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tyr recombinase" evidence="3">
        <location>
            <begin position="168"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="239"/>
            <end position="259"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF07203.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="398" mass="45627" checksum="5B1A45241BC4C782" modified="2006-05-30" version="1">MHTRFHMVVVRHRDGERLPILLDADRQPIGWINEYAIQRLRSRLSANSLTKTLHVLGLLWLWAVQHSIPLQVRLLAGDGLSSDEIASQLYPWLRRDFQSRVAVRRLVVAPTTVAQRLQVVMQFVRWHLERVMARLPIGSPELSHVRERLTTMQRTFATAGPRRAEPVHHASPLSEERVSRLLSICRPGNDENPWKAAYQDRNYVIVLVLLLLGIRRGELLKLRVADCVLSGTIPEIRVERSPDDAKDPRRHEPQVKTESRQLPCDRALASLLNAYICRTRRAIPGADKSPFLFLSRDGAPMSLSRVNGLLTQVGAAHAEFRDLHPHCLRSTCATNFRARALRTGLDEERVEKHMMYFFGWRSSDSIRPYVWHAIRRESCELSLAYQSTIFNASMTGIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="124" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LRM7</accession>
    <name>Q1LRM7_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">UvrABC system protein A</fullName>
            <shortName evidence="14">UvrA protein</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Excinuclease ABC subunit A</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="primary">uvrA1</name>
        <name evidence="14" type="synonym">uvrA</name>
        <name evidence="17" type="ordered locus">Rmet_0313</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">The UvrABC repair system catalyzes the recognition and processing of DNA lesions. UvrA is an ATPase and a DNA-binding protein. A damage recognition complex composed of 2 UvrA and 2 UvrB subunits scans DNA for abnormalities. When the presence of a lesion has been verified by UvrB, the UvrA molecules dissociate.</text>
    </comment>
    <comment type="subunit">
        <text evidence="14">Forms a heterotetramer with UvrB during the search for lesions.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="14">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="14">Belongs to the ABC transporter superfamily. UvrA family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07199.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515199.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0313"/>
    <dbReference type="EnsemblBacteria" id="ABF07199">
        <property type="protein sequence ID" value="ABF07199"/>
        <property type="gene ID" value="Rmet_0313"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0313"/>
    <dbReference type="eggNOG" id="COG0178">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001370_0_2_4"/>
    <dbReference type="OMA" id="PFEGIIP"/>
    <dbReference type="OrthoDB" id="152379at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009380">
        <property type="term" value="C:excinuclease repair complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009381">
        <property type="term" value="F:excinuclease ABC activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006289">
        <property type="term" value="P:nucleotide-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009432">
        <property type="term" value="P:SOS response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00205">
        <property type="entry name" value="UvrA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013815">
        <property type="entry name" value="ATP_grasp_subdomain_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004602">
        <property type="entry name" value="UvrA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041552">
        <property type="entry name" value="UvrA_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041102">
        <property type="entry name" value="UvrA_inter"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17755">
        <property type="entry name" value="UvrA_DNA-bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17760">
        <property type="entry name" value="UvrA_inter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00630">
        <property type="entry name" value="uvra"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 14 15" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 14" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 14" id="KW-0227">DNA damage</keyword>
    <keyword evidence="6 14" id="KW-0228">DNA excision</keyword>
    <keyword evidence="13 14" id="KW-0234">DNA repair</keyword>
    <keyword evidence="12 14" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="10 14" id="KW-0267">Excision nuclease</keyword>
    <keyword evidence="2 14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 14 15" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 14" id="KW-0677">Repeat</keyword>
    <keyword evidence="14" id="KW-0742">SOS response</keyword>
    <keyword evidence="11" id="KW-1278">Translocase</keyword>
    <keyword evidence="8 14" id="KW-0862">Zinc</keyword>
    <keyword evidence="7 14" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="ABC transporter" evidence="16">
        <location>
            <begin position="619"/>
            <end position="948"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C4-type" evidence="14">
        <location>
            <begin position="261"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C4-type" evidence="14">
        <location>
            <begin position="751"/>
            <end position="777"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <begin position="31"/>
            <end position="38"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14 15">
        <location>
            <begin position="652"/>
            <end position="659"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="ABF07199.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="955" mass="105458" checksum="4D851827E8704212" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LRN5</accession>
    <name>Q1LRN5_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Lipopolysaccharide export system protein LptA</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">lptA</name>
        <name evidence="6" type="ordered locus">Rmet_0305</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Involved in the assembly of lipopolysaccharide (LPS). Required for the translocation of LPS from the inner membrane to the outer membrane.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Component of the lipopolysaccharide transport and assembly complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Periplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the LptA family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07191.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515191.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0305"/>
    <dbReference type="EnsemblBacteria" id="ABF07191">
        <property type="protein sequence ID" value="ABF07191"/>
        <property type="gene ID" value="Rmet_0305"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0305"/>
    <dbReference type="eggNOG" id="COG1934">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_095993_1_0_4"/>
    <dbReference type="OMA" id="VPTQQME"/>
    <dbReference type="OrthoDB" id="1696422at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042597">
        <property type="term" value="C:periplasmic space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001530">
        <property type="term" value="F:lipopolysaccharide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043165">
        <property type="term" value="P:Gram-negative-bacterium-type cell outer membrane assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015920">
        <property type="term" value="P:lipopolysaccharide transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01914">
        <property type="entry name" value="LPS_assembly_LptA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014340">
        <property type="entry name" value="LptA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005653">
        <property type="entry name" value="OstA-like_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03968">
        <property type="entry name" value="LptD_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03002">
        <property type="entry name" value="outer_YhbN_LptA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0574">Periplasm</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="1 3" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009018932" description="Lipopolysaccharide export system protein LptA" evidence="3">
        <location>
            <begin position="35"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="domain" description="OstA-like_N" evidence="5">
        <location>
            <begin position="45"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="188"/>
            <end position="214"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022764"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF03968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABF07191.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="214" mass="22709" checksum="F7B1F1DCEB08BE81" modified="2006-05-30" version="1" precursor="true">MTASLTTSSCRRTAPALMAAIAAIGLFLAQPAFAERADQTKPLVLEADNASYDDVKQIYTLTGNVVLTKGTMVLKSDAAELRTDPEGYQYAIATSKPGRQAYIRQKRDGVDEYIDGWGDRIEYDGKSEINKLIGNARAARVSGVGAKILDEIRGAVITYDSRNEFYTATGGDNNASAGNPSGRVRAVLSPRANQASAPAGAPLDLKPSSQPAKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="96" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LRR4</accession>
    <name>Q1LRR4_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">ABC-type transporter, permease component: NitT family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">nasE</name>
        <name evidence="8" type="ordered locus">Rmet_0276</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cell membrane</location>
            <topology evidence="5">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the binding-protein-dependent transport system permease family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07162.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0276"/>
    <dbReference type="EnsemblBacteria" id="ABF07162">
        <property type="protein sequence ID" value="ABF07162"/>
        <property type="gene ID" value="Rmet_0276"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0276"/>
    <dbReference type="eggNOG" id="COG0600">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046113_1_1_4"/>
    <dbReference type="OMA" id="CSIWPMI"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015112">
        <property type="term" value="F:nitrate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005889">
        <property type="entry name" value="NtrB"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="SSF161098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01183">
        <property type="entry name" value="ntrB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="1 5" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="48"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="118"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="149"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="175"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="242"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="274"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="7">
        <location>
            <begin position="111"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ABF07162.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="303" mass="32759" checksum="3C3C9B13B911F786" modified="2006-05-30" version="1">MNALAQSSPEGATPMNPEAVDPDAKEQSRRRAQEIAALEQRAARRDALAAFVLKCVPPLIGFALFVLVWHGIATLIPSIPTPGATWNAAVPLFADPFYRNGPNDQGIGWNVLASLARVAAGFGLAAVVGIPAGFLLGRFAFLNAMASPVISLLRPVSPLAWLPIGLLLFKAANPAAIWAIFICSIWPMIINTAVGVTRVPQDYLNVARVLDLSEWKIFRHVLFPAVLPYMLTGVRLSIGTAWLVIVAAEMLTGGTGIGFWLWDEWNNLKVEHIVIAIFIIGVVGLVLEHLLLALARRFSYGNT</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2011-05-03" modified="2022-08-03" version="100" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LRV9</accession>
    <name>TALY_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Tyrosine ammonia-lyase</fullName>
            <shortName evidence="5">CmTAL</shortName>
            <ecNumber>4.3.1.23</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Tyrosine 2,3-aminomutase</fullName>
            <ecNumber>5.4.3.6</ecNumber>
        </alternativeName>
    </protein>
    <gene>
        <name type="ordered locus">Rmet_0231</name>
    </gene>
    <organism>
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2009" name="ChemBioChem" volume="10" first="741" last="750">
            <title>Discovery of additional members of the tyrosine aminomutase enzyme family and the mutational analysis of CmdF.</title>
            <authorList>
                <person name="Krug D."/>
                <person name="Muller R."/>
            </authorList>
            <dbReference type="PubMed" id="19222035"/>
            <dbReference type="DOI" id="10.1002/cbic.200800748"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>CATALYTIC ACTIVITY</scope>
        <scope>BIOPHYSICOCHEMICAL PROPERTIES</scope>
    </reference>
    <comment type="function">
        <text evidence="4">Has ammonia-lyase and, to a lesser extent, aminomutase activity. Catalyzes the rearrangement of L-tyrosine to R-beta-tyrosine and S-beta-tyrosine. Does not accept L-histidine or L-phenylalanine as substrates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>L-tyrosine = (E)-4-coumarate + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:24906"/>
            <dbReference type="ChEBI" id="CHEBI:12876"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:58315"/>
            <dbReference type="EC" id="4.3.1.23"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>L-tyrosine = 3-amino-3-(4-hydroxyphenyl)propanoate</text>
            <dbReference type="Rhea" id="RHEA:15781"/>
            <dbReference type="ChEBI" id="CHEBI:57956"/>
            <dbReference type="ChEBI" id="CHEBI:58315"/>
            <dbReference type="EC" id="5.4.3.6"/>
        </reaction>
    </comment>
    <comment type="biophysicochemical properties">
        <kinetics>
            <KM evidence="4">348 uM for L-tyrosine (tyrosine 2,3-aminomutase activity)</KM>
        </kinetics>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homotetramer; dimer of dimers.</text>
    </comment>
    <comment type="PTM">
        <text evidence="2">Contains an active site 4-methylidene-imidazol-5-one (MIO), which is formed autocatalytically by cyclization and dehydration of residues Ala-Ser-Gly.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the TAL/TAM family.</text>
    </comment>
    <dbReference type="EC" id="4.3.1.23"/>
    <dbReference type="EC" id="5.4.3.6"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07117.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515131.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q1LRV9"/>
    <dbReference type="SMR" id="Q1LRV9"/>
    <dbReference type="STRING" id="266264.Rmet_0231"/>
    <dbReference type="EnsemblBacteria" id="ABF07117">
        <property type="protein sequence ID" value="ABF07117"/>
        <property type="gene ID" value="Rmet_0231"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0231"/>
    <dbReference type="eggNOG" id="COG2986">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014801_4_1_4"/>
    <dbReference type="OMA" id="MYVHSIP"/>
    <dbReference type="OrthoDB" id="715502at2"/>
    <dbReference type="SABIO-RK" id="Q1LRV9"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016841">
        <property type="term" value="F:ammonia-lyase activity"/>
        <property type="evidence" value="ECO:0000314"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050368">
        <property type="term" value="F:tyrosine 2,3-aminomutase activity"/>
        <property type="evidence" value="ECO:0000314"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052883">
        <property type="term" value="F:tyrosine ammonia-lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd00332">
        <property type="entry name" value="PAL-HAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.275.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001106">
        <property type="entry name" value="Aromatic_Lyase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024083">
        <property type="entry name" value="Fumarase/histidase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008948">
        <property type="entry name" value="L-Aspartase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022313">
        <property type="entry name" value="Phe/His_NH3-lyase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022314">
        <property type="entry name" value="Tyr_aminomutase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10362">
        <property type="entry name" value="PTHR10362"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00221">
        <property type="entry name" value="Lyase_aromatic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48557">
        <property type="entry name" value="SSF48557"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03832">
        <property type="entry name" value="Tyr_2_3_mutase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00488">
        <property type="entry name" value="PAL_HISTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0413">Isomerase</keyword>
    <keyword id="KW-0456">Lyase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000407374" description="Tyrosine ammonia-lyase">
        <location>
            <begin position="1"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="1">
        <location>
            <position position="57"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="87"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="200"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="305"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="modified residue" description="2,3-didehydroalanine (Ser)" evidence="2 3">
        <location>
            <position position="147"/>
        </location>
    </feature>
    <feature type="cross-link" description="5-imidazolinone (Ala-Gly)" evidence="2">
        <location>
            <begin position="146"/>
            <end position="148"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000250" key="2">
        <source>
            <dbReference type="UniProtKB" id="P21310"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="4">
        <source>
            <dbReference type="PubMed" id="19222035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000303" key="5">
        <source>
            <dbReference type="PubMed" id="19222035"/>
        </source>
    </evidence>
    <sequence length="533" mass="56163" checksum="37618DC17B19CC44" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="57" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LS10</accession>
    <name>Q1LS10_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_0180</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07066.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008641842.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0180"/>
    <dbReference type="EnsemblBacteria" id="ABF07066">
        <property type="protein sequence ID" value="ABF07066"/>
        <property type="gene ID" value="Rmet_0180"/>
    </dbReference>
    <dbReference type="GeneID" id="60822903"/>
    <dbReference type="KEGG" id="rme:Rmet_0180"/>
    <dbReference type="eggNOG" id="ENOG5033CJ3">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_181383_2_0_4"/>
    <dbReference type="OMA" id="CWPWLRK"/>
    <dbReference type="OrthoDB" id="2032133at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021320">
        <property type="entry name" value="DUF2905"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11146">
        <property type="entry name" value="DUF2905"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="44"/>
            <end position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF07066.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="64" mass="7205" checksum="13711ABB0758ABAA" modified="2006-05-30" version="1">MLRWTFTIFLCVIVLSASLPWLQKIGLGRLPGDVRFRLFGREYVLPFASTILLSLAALVIGKLL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-01-15" modified="2022-08-03" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LSI9</accession>
    <name>DNAA_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Chromosomal replication initiator protein DnaA</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">dnaA</name>
        <name type="ordered locus">Rmet_0001</name>
    </gene>
    <organism>
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Plays an important role in the initiation and regulation of chromosomal replication. Binds to the origin of replication; it binds specifically double-stranded DNA at a 9 bp consensus (dnaA box): 5'-TTATC[CA]A[CA]A-3'. DnaA binds to ATP and to acidic phospholipids.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the DnaA family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF06887.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011514936.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q1LSI9"/>
    <dbReference type="SMR" id="Q1LSI9"/>
    <dbReference type="STRING" id="266264.Rmet_0001"/>
    <dbReference type="EnsemblBacteria" id="ABF06887">
        <property type="protein sequence ID" value="ABF06887"/>
        <property type="gene ID" value="Rmet_0001"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0001"/>
    <dbReference type="eggNOG" id="COG0593">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026910_0_1_4"/>
    <dbReference type="OMA" id="REFNPLF"/>
    <dbReference type="OrthoDB" id="219876at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003688">
        <property type="term" value="F:DNA replication origin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006270">
        <property type="term" value="P:DNA replication initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006275">
        <property type="term" value="P:regulation of DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd06571">
        <property type="entry name" value="Bac_DnaA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1750.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.180">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00377">
        <property type="entry name" value="DnaA_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001957">
        <property type="entry name" value="Chromosome_initiator_DnaA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020591">
        <property type="entry name" value="Chromosome_initiator_DnaA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018312">
        <property type="entry name" value="Chromosome_initiator_DnaA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013317">
        <property type="entry name" value="DnaA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013159">
        <property type="entry name" value="DnaA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024633">
        <property type="entry name" value="DnaA_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038454">
        <property type="entry name" value="DnaA_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010921">
        <property type="entry name" value="Trp_repressor/repl_initiator"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30050:SF2">
        <property type="entry name" value="PTHR30050:SF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00308">
        <property type="entry name" value="Bac_DnaA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08299">
        <property type="entry name" value="Bac_DnaA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11638">
        <property type="entry name" value="DnaA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00051">
        <property type="entry name" value="DNAA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00760">
        <property type="entry name" value="Bac_DnaA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48295">
        <property type="entry name" value="SSF48295"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00362">
        <property type="entry name" value="DnaA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01008">
        <property type="entry name" value="DNAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0235">DNA replication</keyword>
    <keyword id="KW-0238">DNA-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_1000048700" description="Chromosomal replication initiator protein DnaA">
        <location>
            <begin position="1"/>
            <end position="579"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="131"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="212"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="166"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="284"/>
            <end position="291"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00377"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="579" mass="63551" checksum="A886C87BD81EE857" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2005-04-26" modified="2022-08-03" version="101" xmlns="http://uniprot.org/uniprot">
    <accession>Q58AF4</accession>
    <name>Q58AF4_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">SilA, pump of the three components proton antiporter cation efflux system involved in silver, copper resistance</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">silA</name>
        <name evidence="4" type="ordered locus">Rmet_6136</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4 5">
        <name>pMOL30</name>
    </geneLocation>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="5">Plasmid pMOL30</plasmid>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the resistance-nodulation-cell division (RND) (TC 2.A.6) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000354">
        <property type="protein sequence ID" value="ABF12995.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011229391.1">
        <property type="nucleotide sequence ID" value="NC_007971.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_145664.1">
        <property type="nucleotide sequence ID" value="NC_006466.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF12995">
        <property type="protein sequence ID" value="ABF12995"/>
        <property type="gene ID" value="Rmet_6136"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6136"/>
    <dbReference type="HOGENOM" id="CLU_002755_1_2_4"/>
    <dbReference type="OMA" id="EAEYMIT"/>
    <dbReference type="OrthoDB" id="91817at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid pMOL30"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008324">
        <property type="term" value="F:cation transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2090.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027463">
        <property type="entry name" value="AcrB_DN_DC_subdom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001036">
        <property type="entry name" value="Acrflvin-R"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004763">
        <property type="entry name" value="CzcA/CusA/SilA/NccA/HelA/CnrA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32063">
        <property type="entry name" value="PTHR32063"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00873">
        <property type="entry name" value="ACR_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00702">
        <property type="entry name" value="ACRIFLAVINRP"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82714">
        <property type="entry name" value="SSF82714"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00914">
        <property type="entry name" value="2A0601"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0614">Plasmid</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="363"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="446"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="486"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="538"/>
            <end position="555"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="874"/>
            <end position="891"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="898"/>
            <end position="918"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="924"/>
            <end position="948"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="980"/>
            <end position="997"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="1009"/>
            <end position="1035"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF12995.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="1056" mass="114924" checksum="80771F2DDE054AEF" modified="2005-04-26" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2005-01-04" modified="2022-08-03" version="97" xmlns="http://uniprot.org/uniprot">
    <accession>Q5NUU8</accession>
    <name>Q5NUU8_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Mercuric transport protein MerT</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Mercury ion transport protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="primary">merT</name>
        <name evidence="16" type="ordered locus">Rmet_6172</name>
        <name evidence="17" type="ordered locus">Rmet_6345</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="17 18">
        <name>pMOL28</name>
    </geneLocation>
    <geneLocation type="plasmid" evidence="16 18">
        <name>pMOL30</name>
    </geneLocation>
    <reference evidence="17" key="1">
        <citation type="submission" date="2006-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of the chromosome of Ralstonia metallidurans CH34.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Copeland A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Barry K."/>
                <person name="Detter J.C."/>
                <person name="Glavina del Rio T."/>
                <person name="Hammon N."/>
                <person name="Israni S."/>
                <person name="Dalin E."/>
                <person name="Tice H."/>
                <person name="Martinez M."/>
                <person name="Goltsman E."/>
                <person name="Pitluck S."/>
                <person name="Schmutz J."/>
                <person name="Larimer F."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Kim E."/>
                <person name="Mergeay M."/>
                <person name="Benotmane M.A."/>
                <person name="Vallaeys T."/>
                <person name="Michaux A."/>
                <person name="Monchy S."/>
                <person name="Dunn J."/>
                <person name="McCorkle S."/>
                <person name="Taghavi S."/>
                <person name="van der Lelie D."/>
                <person name="Richardson P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="17">CH34</strain>
            <plasmid evidence="17">pMOL28</plasmid>
            <plasmid evidence="16">pMOL30</plasmid>
        </source>
    </reference>
    <reference evidence="18" key="2">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="18">Plasmid pMOL28</plasmid>
            <plasmid evidence="18">Plasmid pMOL30</plasmid>
        </source>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2010-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Benotmane R."/>
                <person name="Lapidus A."/>
                <person name="McCorkle S."/>
                <person name="Monchy S."/>
                <person name="Taghavi S."/>
                <person name="van der Lelie N."/>
                <person name="Dunn J."/>
                <person name="Leys N."/>
                <person name="Mergeay M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="17">CH34</strain>
            <plasmid evidence="17">pMOL28</plasmid>
            <plasmid evidence="16">pMOL30</plasmid>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell inner membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the MerT family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000354">
        <property type="protein sequence ID" value="ABF13031.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP000355">
        <property type="protein sequence ID" value="ABF13204.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_006224622.1">
        <property type="nucleotide sequence ID" value="NC_007972.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_145638.1">
        <property type="nucleotide sequence ID" value="NC_006466.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_161729.1">
        <property type="nucleotide sequence ID" value="NC_006525.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF13031">
        <property type="protein sequence ID" value="ABF13031"/>
        <property type="gene ID" value="Rmet_6172"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF13204">
        <property type="protein sequence ID" value="ABF13204"/>
        <property type="gene ID" value="Rmet_6345"/>
    </dbReference>
    <dbReference type="GeneID" id="61089173"/>
    <dbReference type="KEGG" id="rme:Rmet_6172"/>
    <dbReference type="KEGG" id="rme:Rmet_6345"/>
    <dbReference type="HOGENOM" id="CLU_151729_0_0_4"/>
    <dbReference type="OMA" id="LASTCCI"/>
    <dbReference type="OrthoDB" id="1896057at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid pMOL28"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid pMOL30"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015097">
        <property type="term" value="F:mercury ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003457">
        <property type="entry name" value="Transprt_MerT"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02411">
        <property type="entry name" value="MerT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="7" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="13 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0475">Mercuric resistance</keyword>
    <keyword evidence="11" id="KW-0476">Mercury</keyword>
    <keyword evidence="10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="17" id="KW-0614">Plasmid</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12 15" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="12"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="53"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="91"/>
            <end position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004429"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00017053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00030934"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="ABF13031.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="ABF13204.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="116" mass="12484" checksum="641E5492AE513E6D" modified="2005-01-04" version="1">MSEPKTGRGALFTGGLAAILASACCLGPLVLIALGFSGAWIGNLAVLDPYRPIFIGVALVALFFAWRRIYRQAAACKPGEVCAIPQVRATYKLIFWIVAALVLVALGFPYVMPFFY</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-03-23" modified="2022-05-25" version="50" xmlns="http://uniprot.org/uniprot">
    <accession>D3DXP6</accession>
    <name>D3DXP6_CUPMC</name>
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        <submittedName>
            <fullName evidence="2">Regulator of plasmid copy number (Ribbon-helix-helix)</fullName>
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    <gene>
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        <name evidence="2" type="ordered locus">Rmet_6451</name>
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        <name type="synonym">Ralstonia metallidurans</name>
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        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="ADC45066.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_6451"/>
    <dbReference type="EnsemblBacteria" id="ADC45066">
        <property type="protein sequence ID" value="ADC45066"/>
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    </dbReference>
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    <dbReference type="eggNOG" id="ENOG5032YZW">
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    </dbReference>
    <dbReference type="HOGENOM" id="CLU_185285_0_0_4"/>
    <dbReference type="OrthoDB" id="1917231at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010985">
        <property type="entry name" value="Ribbon_hlx_hlx"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47598">
        <property type="entry name" value="SSF47598"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADC45066.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="94" mass="10328" checksum="929BC5707D5A6A65" modified="2010-03-23" version="1">MTAKPPPSSKRAAKRVGIGARPPANPHAEAWIRQGDADALGKGDLYTARLTLDITPAMRARIKVSAFTQGVTVAELLRGLLEREFPEHRRENTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-03-23" modified="2020-10-07" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>D3DXU8</accession>
    <name>D3DXU8_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_6510</name>
    </gene>
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        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
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        <lineage>
            <taxon>Bacteria</taxon>
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            <taxon>Betaproteobacteria</taxon>
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        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2194778_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADC45118.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="108" mass="11798" checksum="5BE3AA2D28A047E9" modified="2010-03-23" version="1">MTKRLSGAVAALYRQPDDDLRQVYGQLLTEAERLPCLSSVAVISITLPGRQPAGVDGFEHVLRVSFADVNLLTPTLSHRDTNSHAPSSLELNGVERRICPWVAGALYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-03-23" modified="2020-10-07" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>D3DXY1</accession>
    <name>D3DXY1_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
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        <lineage>
            <taxon>Bacteria</taxon>
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        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ADC45151.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_6544"/>
    <dbReference type="EnsemblBacteria" id="ADC45151">
        <property type="protein sequence ID" value="ADC45151"/>
        <property type="gene ID" value="Rmet_6544"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6544"/>
    <dbReference type="HOGENOM" id="CLU_3029133_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADC45151.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="55" mass="6095" checksum="F997B504F3EB1DD3" modified="2010-03-23" version="1">MYKATVGNGLFQRPSSSARPDPAASHYNRGLILGDFRRTGNGAYPDRSVRQWQGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-03-23" modified="2020-10-07" version="23" xmlns="http://uniprot.org/uniprot">
    <accession>D3DY04</accession>
    <name>D3DY04_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_6571</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
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        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ADC45174.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_6571"/>
    <dbReference type="EnsemblBacteria" id="ADC45174">
        <property type="protein sequence ID" value="ADC45174"/>
        <property type="gene ID" value="Rmet_6571"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6571"/>
    <dbReference type="HOGENOM" id="CLU_3383436_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADC45174.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="33" mass="3702" checksum="5314587598BE4597" modified="2010-03-23" version="1">MSVYIKDLTADIFHARVISLLVRLVCAHISITC</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-03-23" modified="2020-10-07" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>D3DY32</accession>
    <name>D3DY32_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_6601</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ADC45202.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADC45202">
        <property type="protein sequence ID" value="ADC45202"/>
        <property type="gene ID" value="Rmet_6601"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6601"/>
    <dbReference type="HOGENOM" id="CLU_2737163_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADC45202.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="71" mass="7957" checksum="B9A48715A840A2AE" modified="2010-03-23" version="1">MPSKRLSLYPLRCTSQATYTPQEALTGPYTSLTASRRGQEPLWPYSPYTHSQGFQNVSNPRHPFADAAVQT</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-03-23" modified="2020-10-07" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>D3DY88</accession>
    <name>D3DY88_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_6658</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ADC45258.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADC45258">
        <property type="protein sequence ID" value="ADC45258"/>
        <property type="gene ID" value="Rmet_6658"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6658"/>
    <dbReference type="HOGENOM" id="CLU_2957386_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADC45258.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="59" mass="6857" checksum="EB7256F49E422C47" modified="2010-03-23" version="1">MASRLPRNWPSAMVELLEVACLMVVSAYVHMDREEKYFPVFGDARGPTSNWRFRNVLVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-03-23" modified="2020-10-07" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>D3DYI3</accession>
    <name>D3DYI3_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_6358</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2 3">
        <name>pMOL30</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid pMOL30</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000354">
        <property type="protein sequence ID" value="ADC45353.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADC45353">
        <property type="protein sequence ID" value="ADC45353"/>
        <property type="gene ID" value="Rmet_6358"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6358"/>
    <dbReference type="HOGENOM" id="CLU_1775883_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid pMOL30"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003043242" evidence="1">
        <location>
            <begin position="26"/>
            <end position="146"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADC45353.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="146" mass="15221" checksum="FC7135671584FA3B" modified="2010-03-23" version="1">MKRRTLKTLTLLVGAALSLARVSHAGMLEDIGAGAYKAIGSPGYTTVIPPGTRLVVGGEATVISGSDLCPSEPVPPSKYLWFMGGHPVDGSGCVVFGPATKSVPVSLYGSGGKKTSETWSVEHLEHKGIPMMRLKRPNGDFVGAEN</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1994-10-01" modified="2022-08-03" version="136" xmlns="http://uniprot.org/uniprot">
    <accession>P37978</accession>
    <accession>P37976</accession>
    <accession>Q5NUX2</accession>
    <accession>Q7B057</accession>
    <name>CNRH_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName>RNA polymerase sigma factor CnrH</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">cnrH</name>
        <name type="ordered locus">Rmet_6207</name>
        <name type="ORF">RMe0086</name>
    </gene>
    <organism>
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid">
        <name>pMOL28</name>
    </geneLocation>
    <reference key="1">
        <citation type="journal article" date="1993" name="J. Bacteriol." volume="175" first="767" last="778">
            <title>Characterization of the inducible nickel and cobalt resistance determinant cnr from pMOL28 of Alcaligenes eutrophus CH34.</title>
            <authorList>
                <person name="Liesegang H."/>
                <person name="Lemke K."/>
                <person name="Siddiqui R.A."/>
                <person name="Schlegel H.G."/>
            </authorList>
            <dbReference type="PubMed" id="8380802"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC DNA]</scope>
    </reference>
    <reference key="2">
        <citation type="submission" date="2004-10" db="EMBL/GenBank/DDBJ databases">
            <title>Sequence and features of the Ralstonia metallidurans CH34 heavy metal plasmids pMOL28 and pMOL30.</title>
            <authorList>
                <person name="van der Lelie D."/>
                <person name="Monchy S."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="Benotmane M."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Mergeay M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference key="3">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <reference key="4">
        <citation type="journal article" date="1994" name="Proc. Natl. Acad. Sci. U.S.A." volume="91" first="7573" last="7577">
            <title>Analysis of the Streptomyces coelicolor sigE gene reveals the existence of a subfamily of eubacterial RNA polymerase sigma factors involved in the regulation of extracytoplasmic functions.</title>
            <authorList>
                <person name="Lonetto M.A."/>
                <person name="Brown K.L."/>
                <person name="Rudd K.E."/>
                <person name="Buttner M.J."/>
            </authorList>
            <dbReference type="PubMed" id="8052622"/>
            <dbReference type="DOI" id="10.1073/pnas.91.16.7573"/>
        </citation>
        <scope>IDENTIFICATION</scope>
        <scope>SIMILARITY TO OTHER ECF SIGMA FACTORS</scope>
    </reference>
    <reference key="5">
        <citation type="journal article" date="2000" name="J. Bacteriol." volume="182" first="1390" last="1398">
            <title>Regulation of the cnr cobalt and nickel resistance determinant from Ralstonia sp. strain CH34.</title>
            <authorList>
                <person name="Grass G."/>
                <person name="Grosse C."/>
                <person name="Nies D.H."/>
            </authorList>
            <dbReference type="PubMed" id="10671463"/>
            <dbReference type="DOI" id="10.1128/JB.182.5.1390-1398.2000"/>
        </citation>
        <scope>CHARACTERIZATION</scope>
        <scope>INDUCTION</scope>
    </reference>
    <reference key="6">
        <citation type="journal article" date="2000" name="J. Bacteriol." volume="182" first="1399" last="1409">
            <title>Regulation of the cnr cobalt and nickel resistance determinant of Ralstonia eutropha (Alcaligenes eutrophus) CH34.</title>
            <authorList>
                <person name="Tibazarwa C."/>
                <person name="Wuertz S."/>
                <person name="Mergeay M."/>
                <person name="Wyns L."/>
                <person name="van Der Lelie D."/>
            </authorList>
            <dbReference type="PubMed" id="10671464"/>
            <dbReference type="DOI" id="10.1128/JB.182.5.1399-1409.2000"/>
        </citation>
        <scope>DNA-BINDING</scope>
        <scope>INDUCTION</scope>
    </reference>
    <comment type="function">
        <text>Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor regulates the genes for a membrane-located efflux system that confers resistance to nickel and cobalt.</text>
    </comment>
    <comment type="function">
        <text evidence="2 3">CnrH alone is able to activate CNR expression, while both CnrY and CrnX are needed for nickel induction of cnrH (PubMed:10671463). Binds DNA in an RNA polymerase-dependent fashion (PubMed:10671464). CnrH may be controlled by a CnrYX transmembrane anti-sigma factor complex which binds CnrH in the absence of Ni(2+). If Ni(2+) appears in the periplasm, it may be bound by CnrR (CnrX); the signal then would be transmitted by CnrY into the cytoplasm and CnrH would be released.</text>
    </comment>
    <comment type="induction">
        <text evidence="2 3">By nickel and cobalt.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the sigma-70 factor family. ECF subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="M91650">
        <property type="protein sequence ID" value="AAA21967.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AJ276513">
        <property type="protein sequence ID" value="CAB82450.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="X90708">
        <property type="protein sequence ID" value="CAI30230.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP000355">
        <property type="protein sequence ID" value="ABF13066.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011239969.1">
        <property type="nucleotide sequence ID" value="NC_007972.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_161708.1">
        <property type="nucleotide sequence ID" value="NC_006525.1"/>
    </dbReference>
    <dbReference type="PDB" id="4CXF">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="1.75 A"/>
        <property type="chains" value="A=1-191"/>
    </dbReference>
    <dbReference type="PDBsum" id="4CXF"/>
    <dbReference type="AlphaFoldDB" id="P37978"/>
    <dbReference type="SMR" id="P37978"/>
    <dbReference type="PRIDE" id="P37978"/>
    <dbReference type="EnsemblBacteria" id="ABF13066">
        <property type="protein sequence ID" value="ABF13066"/>
        <property type="gene ID" value="Rmet_6207"/>
    </dbReference>
    <dbReference type="GeneID" id="60825775"/>
    <dbReference type="KEGG" id="rme:Rmet_6207"/>
    <dbReference type="HOGENOM" id="CLU_047691_3_0_4"/>
    <dbReference type="OMA" id="WHALDDF"/>
    <dbReference type="OrthoDB" id="1914729at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid pMOL28"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016987">
        <property type="term" value="F:sigma factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006352">
        <property type="term" value="P:DNA-templated transcription, initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006950">
        <property type="term" value="P:response to stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039425">
        <property type="entry name" value="RNA_pol_sigma-70-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014284">
        <property type="entry name" value="RNA_pol_sigma-70_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000838">
        <property type="entry name" value="RNA_pol_sigma70_ECF_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007627">
        <property type="entry name" value="RNA_pol_sigma70_r2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013249">
        <property type="entry name" value="RNA_pol_sigma70_r4_t2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013325">
        <property type="entry name" value="RNA_pol_sigma_r2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013324">
        <property type="entry name" value="RNA_pol_sigma_r3/r4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43133">
        <property type="entry name" value="PTHR43133"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04542">
        <property type="entry name" value="Sigma70_r2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08281">
        <property type="entry name" value="Sigma70_r4_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88659">
        <property type="entry name" value="SSF88659"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88946">
        <property type="entry name" value="SSF88946"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02937">
        <property type="entry name" value="sigma70-ECF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01063">
        <property type="entry name" value="SIGMA70_ECF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0170">Cobalt</keyword>
    <keyword id="KW-0238">DNA-binding</keyword>
    <keyword id="KW-0533">Nickel</keyword>
    <keyword id="KW-0614">Plasmid</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0731">Sigma factor</keyword>
    <keyword id="KW-0804">Transcription</keyword>
    <keyword id="KW-0805">Transcription regulation</keyword>
    <feature type="chain" id="PRO_0000094009" description="RNA polymerase sigma factor CnrH">
        <location>
            <begin position="1"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="H-T-H motif" evidence="1">
        <location>
            <begin position="156"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Polymerase core binding">
        <location>
            <begin position="49"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="helix" evidence="5">
        <location>
            <begin position="6"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="helix" evidence="5">
        <location>
            <begin position="19"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="helix" evidence="5">
        <location>
            <begin position="44"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="helix" evidence="5">
        <location>
            <begin position="62"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="helix" evidence="5">
        <location>
            <begin position="71"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="helix" evidence="5">
        <location>
            <begin position="126"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="helix" evidence="5">
        <location>
            <begin position="140"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="helix" evidence="5">
        <location>
            <begin position="156"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="helix" evidence="5">
        <location>
            <begin position="167"/>
            <end position="188"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000269" key="2">
        <source>
            <dbReference type="PubMed" id="10671463"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="3">
        <source>
            <dbReference type="PubMed" id="10671464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="4"/>
    <evidence type="ECO:0007829" key="5">
        <source>
            <dbReference type="PDB" id="4CXF"/>
        </source>
    </evidence>
    <sequence length="191" mass="20623" checksum="711DE56029AB4816" modified="1994-10-01" version="1">MNPEDADRILAAQAASGNQRAFGQLVARHGVALAQAARSFGIPETDVDDVVQDTFVAAWHALDDFDPDRPFRAWLFRIGLNKMRDLYRFRRVRQFLFGAENLGDLELAGGVANDEPGPEQQVAARLELARVASTLGKLDTGSREVIVLTAIVGMSQPEAAAVLGLSVKAVEGRIGRARAKLSALLDADSEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>Q1L9R4</accession>
    <name>Q1L9R4_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Putative integrase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Rmet_6253</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="8 9">
        <name>pMOL28</name>
    </geneLocation>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="9">Plasmid pMOL28</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000355">
        <property type="protein sequence ID" value="ABF13112.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF13112">
        <property type="protein sequence ID" value="ABF13112"/>
        <property type="gene ID" value="Rmet_6253"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6253"/>
    <dbReference type="HOGENOM" id="CLU_036297_1_0_4"/>
    <dbReference type="OMA" id="YACQRQI"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid pMOL28"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.443.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044068">
        <property type="entry name" value="CB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011010">
        <property type="entry name" value="DNA_brk_join_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013762">
        <property type="entry name" value="Integrase-like_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002104">
        <property type="entry name" value="Integrase_catalytic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010998">
        <property type="entry name" value="Integrase_recombinase_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00589">
        <property type="entry name" value="Phage_integrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56349">
        <property type="entry name" value="SSF56349"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51900">
        <property type="entry name" value="CB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51898">
        <property type="entry name" value="TYR_RECOMBINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0229">DNA integration</keyword>
    <keyword evidence="3" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="2 4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8" id="KW-0614">Plasmid</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Core-binding (CB)" evidence="7">
        <location>
            <begin position="59"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="domain" description="Tyr recombinase" evidence="6">
        <location>
            <begin position="178"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="161"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="165"/>
            <end position="179"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ABF13112.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="552" mass="63063" checksum="4BAEEDF9FE5B7C16" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-10-07" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>Q1L9S1</accession>
    <name>Q1L9S1_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_6246</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2 3">
        <name>pMOL28</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid pMOL28</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000355">
        <property type="protein sequence ID" value="ABF13105.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF13105">
        <property type="protein sequence ID" value="ABF13105"/>
        <property type="gene ID" value="Rmet_6246"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6246"/>
    <dbReference type="HOGENOM" id="CLU_106297_0_0_4"/>
    <dbReference type="OMA" id="DRVFWHP"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid pMOL28"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF13105.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="227" mass="25773" checksum="518D7BB6BF692DF8" modified="2006-05-30" version="1">MAAKVNGSRTKIPGPHRSQGRRRAKFAAKHFQGRKAGVGRERDRISCAFYRKPMSQVTTSMAREVSLGNRPKNEPQFIAPGEPQYGEIARLDAFIFKRYLDRVFWHPRPDVLRFEVRANPSPVGSIYDVVAIVGKGEGEVWFAEDAVPTRWDYVAITESTDGLGRLQREKAKADGRLPEDYVPFVGDGPVQDYSNLENPEEVEQRRWAVVNRLREANRLARHAAGKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="54" xmlns="http://uniprot.org/uniprot">
    <accession>Q1L9W3</accession>
    <name>Q1L9W3_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">ChrI, regulatory protein, involved in Chromate resistance</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">chrI</name>
        <name evidence="2" type="ordered locus">Rmet_6204</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2 3">
        <name>pMOL28</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid pMOL28</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000355">
        <property type="protein sequence ID" value="ABF13063.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF13063">
        <property type="protein sequence ID" value="ABF13063"/>
        <property type="gene ID" value="Rmet_6204"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6204"/>
    <dbReference type="HOGENOM" id="CLU_053665_2_0_4"/>
    <dbReference type="OMA" id="QWAYNGR"/>
    <dbReference type="OrthoDB" id="1797023at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid pMOL28"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005297">
        <property type="entry name" value="Lipoprotein_repeat"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03640">
        <property type="entry name" value="Lipoprotein_15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="144"/>
            <end position="164"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF13063.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="164" mass="17666" checksum="F675E8319E2E38BB" modified="2006-05-30" version="1">MADVWPAPMELQEFIAPCAYDRALYIRAIVLVAELGLFRRCLMKGLSIALIVMGYLAVPSAVAAAPHASEGILVDEQGMTLYVFAGKSSPDAKSCEGNCERNFPPALAASDDKPVGMLTLVPGRGGKSQWAYQGKPLYRGLMDKKPGDRSGDGLNEVWHSVPVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="69" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LC77</accession>
    <name>Q1LC77_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_5390</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12249.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519795.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5390"/>
    <dbReference type="EnsemblBacteria" id="ABF12249">
        <property type="protein sequence ID" value="ABF12249"/>
        <property type="gene ID" value="Rmet_5390"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5390"/>
    <dbReference type="eggNOG" id="ENOG503096N">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_134428_1_0_4"/>
    <dbReference type="OMA" id="AVEDQEY"/>
    <dbReference type="OrthoDB" id="2021063at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014469">
        <property type="entry name" value="DUF2271"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10029">
        <property type="entry name" value="DUF2271"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004192995" evidence="1">
        <location>
            <begin position="23"/>
            <end position="155"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF12249.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="155" mass="16474" checksum="39E196D00CCD8F2F" modified="2006-05-30" version="1">MKAALPVAMLVGALAVPSLASARQVSFETQMSNYGGDGAYVALYLTDNAGRYQRTLWVAGPKAKYHKNLRDWYRAAGGTAKVDGISGASVGAGKTLRVTLDLADTLIDAGYQVHIDTAVEDMKAYPSEVVVPLTRSGVNKPVSGNGYVKSFRYAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="100" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LCK8</accession>
    <name>Q1LCK8_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Flagellar hook-basal body complex protein FliE</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3 4" type="primary">fliE</name>
        <name evidence="4" type="ordered locus">Rmet_5259</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="5">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="5">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 3">Bacterial flagellum basal body</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 3">Belongs to the FliE family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12118.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519664.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5259"/>
    <dbReference type="EnsemblBacteria" id="ABF12118">
        <property type="protein sequence ID" value="ABF12118"/>
        <property type="gene ID" value="Rmet_5259"/>
    </dbReference>
    <dbReference type="GeneID" id="60826539"/>
    <dbReference type="KEGG" id="rme:Rmet_5259"/>
    <dbReference type="eggNOG" id="COG1677">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_147249_0_2_4"/>
    <dbReference type="OMA" id="QMQVKAL"/>
    <dbReference type="OrthoDB" id="1581972at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009425">
        <property type="term" value="C:bacterial-type flagellum basal body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003774">
        <property type="term" value="F:cytoskeletal motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005198">
        <property type="term" value="F:structural molecule activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071973">
        <property type="term" value="P:bacterial-type flagellum-dependent cell motility"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00724">
        <property type="entry name" value="FliE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001624">
        <property type="entry name" value="FliE"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34653">
        <property type="entry name" value="PTHR34653"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02049">
        <property type="entry name" value="FliE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01006">
        <property type="entry name" value="FLGHOOKFLIE"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00205">
        <property type="entry name" value="fliE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0975">Bacterial flagellum</keyword>
    <keyword evidence="4" id="KW-0966">Cell projection</keyword>
    <keyword evidence="4" id="KW-0969">Cilium</keyword>
    <keyword evidence="4" id="KW-0282">Flagellum</keyword>
    <keyword evidence="4" id="KW-0614">Plasmid</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00724"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF12118.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="106" mass="11390" checksum="5AF223CFE7C60278" modified="2006-05-30" version="1">MTISSIEGMLQQMRATAQVAGGGAAPQEASAATESFAGELQRSLQRLNATQDRAYGKAEAFEMGKAGVSLNDVMIDMQKANISFQTAVQVRNRLTAAYQEMMNMPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-12-02" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LCS0</accession>
    <name>Q1LCS0_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Putative cytochrome c (Class I)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Rmet_5197</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="7">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="7">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12056.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5197"/>
    <dbReference type="EnsemblBacteria" id="ABF12056">
        <property type="protein sequence ID" value="ABF12056"/>
        <property type="gene ID" value="Rmet_5197"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5197"/>
    <dbReference type="eggNOG" id="COG4654">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_133112_2_0_4"/>
    <dbReference type="OrthoDB" id="1939800at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.760.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009056">
        <property type="entry name" value="Cyt_c-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036909">
        <property type="entry name" value="Cyt_c-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002324">
        <property type="entry name" value="Cyt_c_ID"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00606">
        <property type="entry name" value="CYTCHROMECID"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46626">
        <property type="entry name" value="SSF46626"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51007">
        <property type="entry name" value="CYTC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 4" id="KW-0349">Heme</keyword>
    <keyword evidence="3 4" id="KW-0408">Iron</keyword>
    <keyword evidence="2 4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0614">Plasmid</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cytochrome c" evidence="5">
        <location>
            <begin position="23"/>
            <end position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABF12056.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="138" mass="14975" checksum="E4A0337E5A4ED020" modified="2006-05-30" version="1">MSARHALRGPAGRSFRRHDELMKSKMKSKKAFVFAVFSTWAAVLGLLNPAAHAAPSSSEETCRACHALDTVKVGPPFRAIASRYHDDADAVDKLKQSLLQGSSGKWGTAQMPPNPITPAEATRFAHWILQLAAPGQRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-10-07" version="52" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LCT4</accession>
    <name>Q1LCT4_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_5183</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12042.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF12042">
        <property type="protein sequence ID" value="ABF12042"/>
        <property type="gene ID" value="Rmet_5183"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5183"/>
    <dbReference type="eggNOG" id="ENOG502ZI2I">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1748147_0_0_4"/>
    <dbReference type="OMA" id="CHATAGI"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193319" evidence="1">
        <location>
            <begin position="20"/>
            <end position="149"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF12042.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="149" mass="16240" checksum="623F9C1CA10E7627" modified="2006-05-30" version="1">MEKIITTIALTTLCSVAHAGMFSTRTYGDGVENVGDKISDLVTDRFTQVFDANTYSVVVIFQHAQVGQDNVCMAIAGVSRRQSPDKQTLPIPAWRLTSTHLQRNSGTLDYARKEACLLSAIRSAVQDLMQVEPEAVRANSARSLANPAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LD64</accession>
    <name>Q1LD64_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_5050</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11912.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519462.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5050"/>
    <dbReference type="EnsemblBacteria" id="ABF11912">
        <property type="protein sequence ID" value="ABF11912"/>
        <property type="gene ID" value="Rmet_5050"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5050"/>
    <dbReference type="eggNOG" id="ENOG502ZAC8">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_397244_0_0_4"/>
    <dbReference type="OMA" id="DVNRYES"/>
    <dbReference type="OrthoDB" id="221823at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193364" evidence="2">
        <location>
            <begin position="20"/>
            <end position="700"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="21"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="23"/>
            <end position="43"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF11912.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="700" mass="70554" checksum="E129BF849A797D44" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LDF3</accession>
    <name>Q1LDF3_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Fimbrial protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">fimA</name>
        <name evidence="2" type="ordered locus">Rmet_4961</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11823.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519383.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4961"/>
    <dbReference type="EnsemblBacteria" id="ABF11823">
        <property type="protein sequence ID" value="ABF11823"/>
        <property type="gene ID" value="Rmet_4961"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4961"/>
    <dbReference type="eggNOG" id="COG3539">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_088965_2_1_4"/>
    <dbReference type="OMA" id="AEPPTNI"/>
    <dbReference type="OrthoDB" id="951513at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009289">
        <property type="term" value="C:pilus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007155">
        <property type="term" value="P:cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1090">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036937">
        <property type="entry name" value="Adhesion_dom_fimbrial_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008966">
        <property type="entry name" value="Adhesion_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039458">
        <property type="entry name" value="FimA-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16970">
        <property type="entry name" value="FimA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49401">
        <property type="entry name" value="SSF49401"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193376" evidence="1">
        <location>
            <begin position="24"/>
            <end position="184"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF11823.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="184" mass="18367" checksum="EC2C70A9740D25D6" modified="2006-05-30" version="1">MKTKLISATIVAIAAMGAQLAHAAPDGTITFNGNITATTCKINGGTAPVNFTVTMPTVSTTTLNALDATAGRTGFRIALTNCTPATGNVHTFFEAGSTVNLGTGRLINAGSATAVEIELLNKDTSVITVGASDGVGAGLQNSKSVPITAGAANLDYYAQYHSTSAAGSGAGAVSSTVTYDISYN</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LDH1</accession>
    <name>Q1LDH1_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative iron-containing alcohol dehydrogenase</fullName>
            <ecNumber evidence="3">1.1.1.1</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_4943</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EC" id="1.1.1.1" evidence="3"/>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11805.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519365.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4943"/>
    <dbReference type="EnsemblBacteria" id="ABF11805">
        <property type="protein sequence ID" value="ABF11805"/>
        <property type="gene ID" value="Rmet_4943"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4943"/>
    <dbReference type="eggNOG" id="COG1454">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007207_0_0_4"/>
    <dbReference type="OMA" id="PGELMDY"/>
    <dbReference type="OrthoDB" id="1456634at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004022">
        <property type="term" value="F:alcohol dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001670">
        <property type="entry name" value="ADH_Fe/GldA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018211">
        <property type="entry name" value="ADH_Fe_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039697">
        <property type="entry name" value="Alcohol_dehydrogenase_Fe"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11496">
        <property type="entry name" value="PTHR11496"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00465">
        <property type="entry name" value="Fe-ADH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00913">
        <property type="entry name" value="ADH_IRON_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fe-ADH" evidence="2">
        <location>
            <begin position="11"/>
            <end position="376"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF11805.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="385" mass="40365" checksum="31E60CBEBB7AD268" modified="2006-05-30" version="1">MNPFRFQTVPTVIVELGAARRLGALLREAFPQSQRLCVVTDGFLSKSGLLSPALDDLGKHGWQVTLIDDVIADPPEHIVLEATERARQADAEVVLGLGGGSSMDVAKLIAVLLPAAQSLKDMYGVKKVTGTRLPLVQMPTTAGTGSEVTAVSIVTTGETTKMGVVAPQLLADLAILDAELTLGLPRAATAATGIDAMVHAIEAYTSAHLKNPISDMLAVRALELLSQNLMLACDNGHDHGAREAMLLGAMFAGQAFANSPVAAVHALAYPIGGIFHVAHGLSNALVLPHVMKFNAPAASKLYAELADVILPGTTGSCETKTAALIAHIEGLVLATGIPRTLREVGVKENDLGRLARDAMLQTRLLVNNPREVTEADAFAIYSAAF</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LDK7</accession>
    <name>Q1LDK7_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative isomerase/hydrolase putative Fumarylacetoacetate (FAA) hydrolase</fullName>
            <ecNumber evidence="2">3.7.1.2</ecNumber>
            <ecNumber evidence="2">4.1.1.-</ecNumber>
            <ecNumber evidence="2">5.3.3.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_4907</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EC" id="3.7.1.2" evidence="2"/>
    <dbReference type="EC" id="4.1.1.-" evidence="2"/>
    <dbReference type="EC" id="5.3.3.-" evidence="2"/>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11769.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519328.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4907"/>
    <dbReference type="EnsemblBacteria" id="ABF11769">
        <property type="protein sequence ID" value="ABF11769"/>
        <property type="gene ID" value="Rmet_4907"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4907"/>
    <dbReference type="eggNOG" id="COG0179">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028458_5_1_4"/>
    <dbReference type="OMA" id="PPRTENY"/>
    <dbReference type="OrthoDB" id="775108at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004334">
        <property type="term" value="F:fumarylacetoacetase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016853">
        <property type="term" value="F:isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.850.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011234">
        <property type="entry name" value="Fumarylacetoacetase-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036663">
        <property type="entry name" value="Fumarylacetoacetase_C_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01557">
        <property type="entry name" value="FAA_hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56529">
        <property type="entry name" value="SSF56529"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0413">Isomerase</keyword>
    <keyword evidence="2" id="KW-0456">Lyase</keyword>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAA_hydrolase" evidence="1">
        <location>
            <begin position="27"/>
            <end position="228"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF11769.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="231" mass="24487" checksum="154FAAE86626EADA" modified="2006-05-30" version="1">MTEFVIQPAPQASVAVAGSQARFPIRRVFCVGRNYAAHAREMGKDPDREPPFFFTKPADAVVAAEGTVPHPPLTENLHHEIELVVAIGKGGTNVSPAQALDLVWGYAVGVDMTRRDLQDVAKKMSRPWDWSKAFDASGPCGPLHAASSIGHPSKGAIWLKVNGETRQEGDLTELIWPVADVIAYISEAVALEPGDLIFTGTPAGVGALNPGDVVSGGVEGIGEITFTIGKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="92" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LDS3</accession>
    <name>Q1LDS3_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Putative ABC transporter, membrane component</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Rmet_4841</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="7">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="7">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cell membrane</location>
            <topology evidence="4">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the binding-protein-dependent transport system permease family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11703.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008641400.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4841"/>
    <dbReference type="EnsemblBacteria" id="ABF11703">
        <property type="protein sequence ID" value="ABF11703"/>
        <property type="gene ID" value="Rmet_4841"/>
    </dbReference>
    <dbReference type="GeneID" id="60823906"/>
    <dbReference type="KEGG" id="rme:Rmet_4841"/>
    <dbReference type="eggNOG" id="COG0555">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016047_18_6_4"/>
    <dbReference type="OMA" id="CFATSMG"/>
    <dbReference type="OrthoDB" id="1584669at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="SSF161098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0614">Plasmid</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2 4" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="54"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="94"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="136"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="196"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="239"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="5">
        <location>
            <begin position="57"/>
            <end position="260"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABF11703.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="272" mass="28957" checksum="7264A09F63F34A61" modified="2006-05-30" version="1">MTRRSPALFLFALPALVVFLAFFCLPMARLIEVSLTGKDGAGVYWTVLTSGRYLHSLMVTVLLSALVTLATLVIAGISGTFLQRHPVPGKPVLVAMLTFPLAFPGVVIGFMVIMLGGRNGLLASIGDALTGERWTFAYGLAGLFVGYLYFSIPRVILTVMAAVDKLDTSLEEAARSLGASRWHVVRDVMLPALMPAMLSSGAICFATSMGAFGTAFTLATQLDVLPLTIYNEFTNYANFGMAAALSILLGAVTWMLLALARWYSGDAVAATA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="105" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LDS9</accession>
    <name>Q1LDS9_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Peptidoglycan glycosyltransferase</fullName>
            <ecNumber evidence="4">2.4.1.129</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="ordered locus">Rmet_4835</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="21">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="21" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="21">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>[GlcNAc-(1-&gt;4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)](n)-di-trans,octa-cis-undecaprenyl diphosphate + beta-D-GlcNAc-(1-&gt;4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-di-trans,octa-cis-undecaprenyl diphosphate = [GlcNAc-(1-&gt;4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)](n+1)-di-trans-octa-cis-undecaprenyl diphosphate + di-trans,octa-cis-undecaprenyl diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:23708"/>
            <dbReference type="Rhea" id="RHEA-COMP:9602"/>
            <dbReference type="Rhea" id="RHEA-COMP:9603"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:58405"/>
            <dbReference type="ChEBI" id="CHEBI:60033"/>
            <dbReference type="ChEBI" id="CHEBI:78435"/>
            <dbReference type="EC" id="2.4.1.129"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cell wall biogenesis; peptidoglycan biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">In the C-terminal section; belongs to the transpeptidase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">In the N-terminal section; belongs to the glycosyltransferase 51 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.129" evidence="4"/>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11697.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4835"/>
    <dbReference type="CAZy" id="GT51">
        <property type="family name" value="Glycosyltransferase Family 51"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF11697">
        <property type="protein sequence ID" value="ABF11697"/>
        <property type="gene ID" value="Rmet_4835"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4835"/>
    <dbReference type="eggNOG" id="COG5009">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006354_2_4_4"/>
    <dbReference type="OMA" id="HNGVDWK"/>
    <dbReference type="UniPathway" id="UPA00219"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004180">
        <property type="term" value="F:carboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008658">
        <property type="term" value="F:penicillin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008955">
        <property type="term" value="F:peptidoglycan glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009252">
        <property type="term" value="P:peptidoglycan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008360">
        <property type="term" value="P:regulation of cell shape"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3810.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.710.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012338">
        <property type="entry name" value="Beta-lactam/transpept-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001264">
        <property type="entry name" value="Glyco_trans_51"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023346">
        <property type="entry name" value="Lysozyme-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036950">
        <property type="entry name" value="PBP_transglycosylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001460">
        <property type="entry name" value="PCN-bd_Tpept"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00912">
        <property type="entry name" value="Transgly"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00905">
        <property type="entry name" value="Transpeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53955">
        <property type="entry name" value="SSF53955"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56601">
        <property type="entry name" value="SSF56601"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0121">Carboxypeptidase</keyword>
    <keyword evidence="10" id="KW-0133">Cell shape</keyword>
    <keyword evidence="14" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="7 20" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="17" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="11" id="KW-0573">Peptidoglycan synthesis</keyword>
    <keyword evidence="20" id="KW-0614">Plasmid</keyword>
    <keyword evidence="6" id="KW-0645">Protease</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="20" id="KW-0808">Transferase</keyword>
    <keyword evidence="8 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12 17" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="29"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="domain" description="Transgly" evidence="19">
        <location>
            <begin position="73"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="domain" description="Transpeptidase" evidence="18">
        <location>
            <begin position="401"/>
            <end position="632"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="723"/>
            <end position="750"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="763"/>
            <end position="826"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="16">
        <location>
            <begin position="785"/>
            <end position="810"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004752"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007739"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023988"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF00905"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF00912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="ABF11697.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="826" mass="90318" checksum="E2E4DB40C7129B9A" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="95" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LDT8</accession>
    <name>Q1LDT8_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Multidrug efflux system protein drug resistance MFS transporter EmrB/QacA subfamily</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">mdtD</name>
        <name evidence="7" type="ordered locus">Rmet_4826</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="8">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="8">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11688.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4826"/>
    <dbReference type="EnsemblBacteria" id="ABF11688">
        <property type="protein sequence ID" value="ABF11688"/>
        <property type="gene ID" value="Rmet_4826"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4826"/>
    <dbReference type="eggNOG" id="COG2814">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000960_28_0_4"/>
    <dbReference type="OMA" id="TALFMEN"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
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        <property type="entry name" value="EmrB-like"/>
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        <property type="entry name" value="MFS"/>
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        <property type="entry name" value="MFS_dom"/>
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        <property type="entry name" value="MFS_1"/>
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        <property type="entry name" value="SSF103473"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
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    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
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        <location>
            <begin position="21"/>
            <end position="40"/>
        </location>
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    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
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        <location>
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    <feature type="transmembrane region" description="Helical" evidence="5">
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        </location>
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    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
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        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
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        </location>
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    <feature type="transmembrane region" description="Helical" evidence="5">
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            <end position="226"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
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            <end position="255"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
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            <end position="296"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="341"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="365"/>
            <end position="385"/>
        </location>
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    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="406"/>
            <end position="428"/>
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            <begin position="440"/>
            <end position="462"/>
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        <location>
            <begin position="22"/>
            <end position="469"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABF11688.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="484" mass="51981" checksum="2AB7F3DD5DCBD7CA" modified="2006-05-30" version="1">MSTATPADHPSTMPIDRRLRFMLWVVAVGFFMQTLDSTIVNTALPSMAHSLGESPLRMQSVIIAYSLTMAVIIPASGWLADKFGTRTIFQIAIVLFVAGSLLCAAAPSLPFLTAARVVQGAGGAMLLPVGRLSVLRTFPREQYLQALSFVAIPGMIGPLIGPTLGGWITQTFSWHWIFLINVPVGLLGVLATIRYMPNSRLPGIGTFDAVGYAFLAIAMVTLSFSLDGLAEMGFQHATVLMLLIASMAALSAYVLHANKRKQPLFPLRLFRIHSFSVGLLGNLFARIGNGAMPFLIPLTLQVSLGYSPFDAGLMMLPVTAAGMASKRLATWLIERHGYRRVLVTNTFVVGLVMASFALITPTRPLALIVVLLAIFGAVNSIQFTAMNTVTLKDLSGTRASSGNSMLSMVQMLSMSLAVTSAGALLATFQEGIGEHHLDVLPAFHATFVCVGLITSASAWIFMQLSPEKAERHKAREDVKQGGEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-10-07" version="58" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LDW5</accession>
    <name>Q1LDW5_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_4799</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11661.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4799"/>
    <dbReference type="EnsemblBacteria" id="ABF11661">
        <property type="protein sequence ID" value="ABF11661"/>
        <property type="gene ID" value="Rmet_4799"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4799"/>
    <dbReference type="HOGENOM" id="CLU_1676646_0_0_4"/>
    <dbReference type="OMA" id="ENHASEY"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF11661.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="177" mass="20533" checksum="4A5A70478F0FAD97" modified="2006-05-30" version="1">MTTEDQKSQIQKAKRPTEGAMNNSDTSAYRKALLYRRANGRKIISSHLHKVAEICPSSDHKNLISLEETDSLLVEFNSRRTALLEFRRRISPHEIQEVLETISKTYKGGAYIFIDEDWRYCGALRIAESWMINKEFRFGPKIINDLIFIDVGMEKAISLDYFEINGDTLVDVIEWEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LDX0</accession>
    <name>Q1LDX0_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative transcriptional regulator, MarR family, involved in virulence</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">slyA</name>
        <name evidence="2" type="ordered locus">Rmet_4794</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11656.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008651197.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4794"/>
    <dbReference type="EnsemblBacteria" id="ABF11656">
        <property type="protein sequence ID" value="ABF11656"/>
        <property type="gene ID" value="Rmet_4794"/>
    </dbReference>
    <dbReference type="GeneID" id="60821618"/>
    <dbReference type="KEGG" id="rme:Rmet_4794"/>
    <dbReference type="eggNOG" id="COG1846">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_083287_18_2_4"/>
    <dbReference type="OMA" id="HLTAHAW"/>
    <dbReference type="OrthoDB" id="1744037at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000835">
        <property type="entry name" value="HTH_MarR-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12802">
        <property type="entry name" value="MarR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00598">
        <property type="entry name" value="HTHMARR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00347">
        <property type="entry name" value="HTH_MARR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50995">
        <property type="entry name" value="HTH_MARR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH marR-type" evidence="1">
        <location>
            <begin position="5"/>
            <end position="141"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF11656.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="152" mass="16242" checksum="DEDDA87E7468207F" modified="2006-05-30" version="1">MPKIDALLFAFTGQLMLAGRQWRQISQAAVTAHGISAAAAAPLLFIRRLGGGVRQVTLADYVGVEGATLVRLVDQLSAAGLVRREVDSSDRRANVLSLTEEGERMAELIEVALKQLRTEVFADISESDMAATLRVFDALARAAASTSGKQES</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="97" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LE33</accession>
    <name>Q1LE33_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Transcriptional regulator, AraC family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Rmet_4731</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="6">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="6">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11593.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4731"/>
    <dbReference type="EnsemblBacteria" id="ABF11593">
        <property type="protein sequence ID" value="ABF11593"/>
        <property type="gene ID" value="Rmet_4731"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4731"/>
    <dbReference type="eggNOG" id="COG2207">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_81_0_4"/>
    <dbReference type="OMA" id="PFLMVEV"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032783">
        <property type="entry name" value="AraC_lig"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018060">
        <property type="entry name" value="HTH_AraC"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12852">
        <property type="entry name" value="Cupin_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12833">
        <property type="entry name" value="HTH_18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00342">
        <property type="entry name" value="HTH_ARAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01124">
        <property type="entry name" value="HTH_ARAC_FAMILY_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0614">Plasmid</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH araC/xylS-type" evidence="4">
        <location>
            <begin position="187"/>
            <end position="284"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS01124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABF11593.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="289" mass="31854" checksum="02CC30E1C7B0C3C4" modified="2006-05-30" version="1">MSHPIESTPDRLSGLLERFRVRAHLFHTGALCGISRFDVKERGFLHVLREGHLTLSHSARGLPRRMEIREPSLLFYPRHVVHTFHNPPIEGSQFTCATLEFAGGAMNPVARALPPLIVLPLARVEGLDAALSLLFAETGRVRCGQRLLADRLFEVVLIQLLRWMLDHPADAGIQAGLITGLSDPRLARALVAMHEAPGETWNLDRMASCAGMSRSAFASAFREIVGQTPADYLAGWRISLAQARLRDGVPIKLLANELGYANPSALSRVFAAKVGKSPREWLQTQASEH</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LE34</accession>
    <name>Q1LE34_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative alkylhydroperoxidase-like protein, AhpD family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_4730</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11592.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF11592">
        <property type="protein sequence ID" value="ABF11592"/>
        <property type="gene ID" value="Rmet_4730"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4730"/>
    <dbReference type="eggNOG" id="COG2128">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_082760_5_0_4"/>
    <dbReference type="OMA" id="DYCLSAH"/>
    <dbReference type="OrthoDB" id="1925754at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004601">
        <property type="term" value="F:peroxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051920">
        <property type="term" value="F:peroxiredoxin activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1290.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029032">
        <property type="entry name" value="AhpD-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004675">
        <property type="entry name" value="AhpD_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003779">
        <property type="entry name" value="CMD-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010195">
        <property type="entry name" value="Uncharacterised_peroxidase-rel"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02627">
        <property type="entry name" value="CMD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69118">
        <property type="entry name" value="SSF69118"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00778">
        <property type="entry name" value="ahpD_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01926">
        <property type="entry name" value="peroxid_rel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="2" id="KW-0575">Peroxidase</keyword>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CMD" evidence="1">
        <location>
            <begin position="96"/>
            <end position="167"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02627"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF11592.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="225" mass="23933" checksum="BB526A8730F01D81" modified="2006-05-30" version="1">MNWYVSWTYGIRSCCARRHSGAHGRHGPSGSQYHSTYHPERSFIMTARVPLVDQRTAPAASVTAIEQIQTAFGVVPNMFRAVANSPAALQSMWGSFGALGGGTISAQLGEKIAVAVADRNRCEYCLAAHTALGRKAGASAEEMAAAQAGLSDEPKTAAALAFAVKVVEARGQISETEVASLRQVGFGDEEIVEILAHVALNLFTNYVNVVFNVPVDFPGVKLIAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LE70</accession>
    <name>Q1LE70_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Putative PpiC-type peptidyl-prolyl cis-trans isomerase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Rmet_4694</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="5">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="5">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11556.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4694"/>
    <dbReference type="EnsemblBacteria" id="ABF11556">
        <property type="protein sequence ID" value="ABF11556"/>
        <property type="gene ID" value="Rmet_4694"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4694"/>
    <dbReference type="eggNOG" id="COG0760">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_034646_1_1_4"/>
    <dbReference type="OMA" id="AYEKHIA"/>
    <dbReference type="OrthoDB" id="1838755at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003755">
        <property type="term" value="F:peptidyl-prolyl cis-trans isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.50.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046357">
        <property type="entry name" value="PPIase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000297">
        <property type="entry name" value="PPIase_PpiC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027304">
        <property type="entry name" value="Trigger_fact/SurA_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13145">
        <property type="entry name" value="Rotamase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109998">
        <property type="entry name" value="SSF109998"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50198">
        <property type="entry name" value="PPIC_PPIASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 4" id="KW-0413">Isomerase</keyword>
    <keyword evidence="4" id="KW-0614">Plasmid</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0697">Rotamase</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5007923078" evidence="2">
        <location>
            <begin position="31"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="domain" description="PpiC" evidence="3">
        <location>
            <begin position="148"/>
            <end position="247"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF11556.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="291" mass="31483" checksum="CD9CAF99CA7A027A" modified="2006-05-30" version="1">MNAIALSRLSGPARLALGLMLAACLAPCQAADDSGSGAGTGLPPGTLAVVNGVPIAQTQLDDALRVARQADTPQMRQFFTQELIVREVLRQSAEKDGYDRRPEVVQAANTAKASAEAELYLRDNLRLDPITDAQVKARYDEVVSMMGKEEYKVRIISVADDRTARTVLEKLRAGVSFDELVRMYSVASSKEGGGEMPWITFRVPVVEGKSQGVPVAVAEAVSHLEVGAVTQEPVVVDNLRVIVRLDAKRATQVPAFDDVKDDIRRQLHIVALNKARADVVGNLMQAATIRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="64" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LED9</accession>
    <name>Q1LED9_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Gly-zipper_YMGG domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_4625</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11487.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519063.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4625"/>
    <dbReference type="EnsemblBacteria" id="ABF11487">
        <property type="protein sequence ID" value="ABF11487"/>
        <property type="gene ID" value="Rmet_4625"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4625"/>
    <dbReference type="eggNOG" id="ENOG50330H2">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_126483_0_0_4"/>
    <dbReference type="OMA" id="NDDGACY"/>
    <dbReference type="OrthoDB" id="2075667at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027367">
        <property type="entry name" value="Gly-zipper_YMGG"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13441">
        <property type="entry name" value="Gly-zipper_YMGG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Gly-zipper_YMGG" evidence="1">
        <location>
            <begin position="83"/>
            <end position="126"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF11487.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="165" mass="16664" checksum="AB78B9D1F84C8C59" modified="2006-05-30" version="1">MSNRPDFALAACTAIVLISGALPGLARAQMIPQKPIAYPARGQSSYQQSNDDGACYSWARQQTGIDPLQIASSPVPVIVPGGERVRGAAGGAAAGAVVGAIAGDAGKGAAAGAAAGTIAGGVAHRQQRRQAVATSAQIQANNGWAVTSYWQAWRACMTGRGYSVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LEK4</accession>
    <name>Q1LEK4_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative membrane protein DUF6</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_4560</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11422.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519011.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4560"/>
    <dbReference type="EnsemblBacteria" id="ABF11422">
        <property type="protein sequence ID" value="ABF11422"/>
        <property type="gene ID" value="Rmet_4560"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4560"/>
    <dbReference type="eggNOG" id="COG0697">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033863_5_0_4"/>
    <dbReference type="OMA" id="WAVCILH"/>
    <dbReference type="OrthoDB" id="1515911at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000620">
        <property type="entry name" value="EamA_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00892">
        <property type="entry name" value="EamA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="21"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="50"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="82"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="109"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="137"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="164"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="197"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="228"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="257"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="284"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="domain" description="EamA" evidence="2">
        <location>
            <begin position="25"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="domain" description="EamA" evidence="2">
        <location>
            <begin position="166"/>
            <end position="300"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF11422.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="312" mass="32866" checksum="A5F3AA5750AFA213" modified="2006-05-30" version="1">MSATVPSTASSRAPATTGFRSFLPLIGAVVIWGGNWPVMKMGLAHISPLWFAACRFGSAALISFLVLGALRRLRLPSRQEWPLVIGVGLLQMGAFTALALWALQYVPPGRASVIAYATAIWVIPLSSLVLGERPSRLQWLATAFSYAGIAVIVAPALGGWELHTVMGLTMLLGASLAWSVSIIQLRANRAIRLGADMIPWECAVATVPLIALAWLRDGAPDLGAMVDIWPVIAYTGPLATALTFIVVLNVTQSLPPAATSIAMLGVPVIGLVLSTVAWHEQISLDLSIGLALIGMGVVTTALAPRLGRLAAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LEV3</accession>
    <name>Q1LEV3_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Acyl-CoA dehydrogenase-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">acdA</name>
        <name evidence="8" type="ordered locus">Rmet_4458</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="9">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="9">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the acyl-CoA dehydrogenase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11323.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518926.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4458"/>
    <dbReference type="EnsemblBacteria" id="ABF11323">
        <property type="protein sequence ID" value="ABF11323"/>
        <property type="gene ID" value="Rmet_4458"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4458"/>
    <dbReference type="eggNOG" id="COG1960">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018204_0_2_4"/>
    <dbReference type="OMA" id="GFPHDFH"/>
    <dbReference type="OrthoDB" id="760677at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016627">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-CH group of donors"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.540.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.110.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006091">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046373">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036250">
        <property type="entry name" value="AcylCo_DH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009075">
        <property type="entry name" value="AcylCo_DH/oxidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013786">
        <property type="entry name" value="AcylCoA_DH/ox_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037069">
        <property type="entry name" value="AcylCoA_DH/ox_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009100">
        <property type="entry name" value="AcylCoA_DH/oxidase_NM_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00441">
        <property type="entry name" value="Acyl-CoA_dh_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02770">
        <property type="entry name" value="Acyl-CoA_dh_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02771">
        <property type="entry name" value="Acyl-CoA_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47203">
        <property type="entry name" value="SSF47203"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56645">
        <property type="entry name" value="SSF56645"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0274">FAD</keyword>
    <keyword evidence="3" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="8" id="KW-0614">Plasmid</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Acyl-CoA_dh_N" evidence="7">
        <location>
            <begin position="6"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA_dh_M" evidence="6">
        <location>
            <begin position="123"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA_dh_1" evidence="5">
        <location>
            <begin position="235"/>
            <end position="382"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF02770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF02771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ABF11323.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="390" mass="42309" checksum="03A61E2BCBE24A0F" modified="2006-05-30" version="1">MNFERTPEQNAIVEAVTQLCSDFGAEYWAELDREHKFPEAFHRAMADAGWLGIAMPERFGGAGLGITEAALIMQTVSASGAGFSGASSIHMNVFGLNPVVVFGTEAQQAAALPPLIAGRDKACFAVTEPDAGLDTTHLKTQAVRTADGSAYRVDGRKIWISTAQVASRMLLLARTTPLDAVAKPTQGLSLFYTTLDRNHIEVREIDKMGRGAVDSNMLFIDGLMVPAEDRIGEEGRGFEYILHGLNPERILIAAESVGLGRAALEAATRYARERTVFGRPIGQNQGIQHPLAQAWMALEAADMMVWKAAWLYDNGKPCGAEANAAKYLAAEAAHQACQTAVLTLGGMGYAREYHVERYLRESYIPRIAPVSAQLIMCHIAERVLGLPKSY</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LEX9</accession>
    <name>Q1LEX9_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Transcriptional regulator, IclR family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Rmet_4432</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="7">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="7">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11297.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4432"/>
    <dbReference type="EnsemblBacteria" id="ABF11297">
        <property type="protein sequence ID" value="ABF11297"/>
        <property type="gene ID" value="Rmet_4432"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4432"/>
    <dbReference type="eggNOG" id="COG1414">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_062618_4_1_4"/>
    <dbReference type="OMA" id="YSLGHRV"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029016">
        <property type="entry name" value="GAF-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014757">
        <property type="entry name" value="Tscrpt_reg_IclR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005471">
        <property type="entry name" value="Tscrpt_reg_IclR_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09339">
        <property type="entry name" value="HTH_IclR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00346">
        <property type="entry name" value="HTH_ICLR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51077">
        <property type="entry name" value="HTH_ICLR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51078">
        <property type="entry name" value="ICLR_ED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0614">Plasmid</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH iclR-type" evidence="4">
        <location>
            <begin position="98"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="domain" description="IclR-ED" evidence="5">
        <location>
            <begin position="160"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="315"/>
            <end position="334"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABF11297.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="334" mass="35473" checksum="DCDFE13196130556" modified="2006-05-30" version="1">MEPAIQPSCSVERNIYSSEQNSSRIMDQKSSTTGARQAKICGRHEATCLCGRTGILLSPWQPVGSQRYVARRPCLFTSSTSDMLQTPPDAAAGQTDGVAAVDRAFAIAVALAQAGAPQTLADLARATGMYKSTLLRLLASLERSSLVVQRPDKRYTLGSLAFLFGRAFEASHGLQEAIYPVMEWLVSKGTESPSFHVPHGPDNRICLLRIDSSHSTLDRVRVGDVLPLRKGAPGKVLIAYGAGAPATADTPVVFTSFGERDPLCGAISSPVFGPGNTLLGALSLSGPLERFSELAVNRMTDLLLEAASRATQALGGRWPDDAAPDAPKAAKRRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="99" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LF20</accession>
    <name>Q1LF20_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">3-ketoacyl-(Acyl-carrier-protein) reductase</fullName>
            <ecNumber evidence="1">1.1.1.100</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">fabG</name>
        <name evidence="1" type="ordered locus">Rmet_4391</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EC" id="1.1.1.100" evidence="1"/>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11256.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518873.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4391"/>
    <dbReference type="EnsemblBacteria" id="ABF11256">
        <property type="protein sequence ID" value="ABF11256"/>
        <property type="gene ID" value="Rmet_4391"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4391"/>
    <dbReference type="eggNOG" id="COG1028">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010194_1_3_4"/>
    <dbReference type="OMA" id="GFCNTEQ"/>
    <dbReference type="OrthoDB" id="1601931at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004316">
        <property type="term" value="F:3-oxoacyl-[acyl-carrier-protein] reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011285">
        <property type="entry name" value="FabG-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01831">
        <property type="entry name" value="fabG_rel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF11256.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="243" mass="25998" checksum="8001A34066A63654" modified="2006-05-30" version="1">MTTQTVLVTGSSRGIGRAIALRLARDGYDVVIHCRARRNEAESVADAVRAFGRNARVLCFDVGQREAAASALLADIDHHGCYYGVVCNAGLARDAAFPAMSGEEWDEVIHTNLDAFFNVLNPVVLPMVQRRRPGRIVTLSSASGIVGNRGQTNYSAAKAGVIGATKALAIELAKRQITVNCVAPGLIDTEMIEEHVREEAMKMIPARRMGTPDEVAATVAFLLSPDAGYITRQVISVNGGMFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LF33</accession>
    <name>Q1LF33_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Acyl carrier protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">acpP</name>
        <name evidence="3" type="ordered locus">Rmet_4378</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11243.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518861.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4378"/>
    <dbReference type="EnsemblBacteria" id="ABF11243">
        <property type="protein sequence ID" value="ABF11243"/>
        <property type="gene ID" value="Rmet_4378"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4378"/>
    <dbReference type="eggNOG" id="COG0236">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_108696_14_1_4"/>
    <dbReference type="OMA" id="FIHAQRA"/>
    <dbReference type="OrthoDB" id="1943389at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1200.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036736">
        <property type="entry name" value="ACP-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009081">
        <property type="entry name" value="PP-bd_ACP"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00550">
        <property type="entry name" value="PP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47336">
        <property type="entry name" value="SSF47336"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50075">
        <property type="entry name" value="CARRIER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0596">Phosphopantetheine</keyword>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Carrier" evidence="2">
        <location>
            <begin position="2"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="modified residue" description="O-(pantetheine 4'-phosphoryl)serine" evidence="1">
        <location>
            <position position="41"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00258"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF11243.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="89" mass="9670" checksum="AFCDCDADC528F14B" modified="2006-05-30" version="1">MKSLEQEIKELIITSLSLEDIKAEDIETDAPLFVEGLGLDSIDALELGLALQKRYGITMKGEKDEVRARFASVATLAAYIASQRGQATA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LFC1</accession>
    <name>Q1LFC1_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Nucleoside-diphosphate-sugar epimerase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">yraR</name>
        <name evidence="2" type="ordered locus">Rmet_4289</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11155.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4289"/>
    <dbReference type="EnsemblBacteria" id="ABF11155">
        <property type="protein sequence ID" value="ABF11155"/>
        <property type="gene ID" value="Rmet_4289"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4289"/>
    <dbReference type="eggNOG" id="COG0702">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_071330_2_1_4"/>
    <dbReference type="OMA" id="FCCIGTT"/>
    <dbReference type="OrthoDB" id="948454at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016040">
        <property type="entry name" value="NAD(P)-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13460">
        <property type="entry name" value="NAD_binding_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAD(P)-bd_dom" evidence="1">
        <location>
            <begin position="14"/>
            <end position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF11155.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="223" mass="23208" checksum="E42478884F253CA5" modified="2006-05-30" version="1">MSTPIDQRKVLLAGATGLVGGLMLQALLADPTVAQVHALSRRPLRIRHPRLQVHIVDFSRLPALPQADEVYLALGTTIKVAGSQAAFRAVDLEANLAVAKAAFAAGASRAGLVSAVGANAKSSTFYSRVKGELEDALRSLGLTTLVIGRPSLLLDSRDGLQQPPRIGEQIAIPIAKLLAPLLPGAYRPVHARAVALSLVKTVPATEGVVILSSSMLASIGSEP</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LFH9</accession>
    <name>Q1LFH9_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Outer membrane protein (Porin)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">omp</name>
        <name evidence="7" type="ordered locus">Rmet_4230</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="8">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="8">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11097.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518720.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4230"/>
    <dbReference type="EnsemblBacteria" id="ABF11097">
        <property type="protein sequence ID" value="ABF11097"/>
        <property type="gene ID" value="Rmet_4230"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4230"/>
    <dbReference type="eggNOG" id="COG3203">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038238_2_0_4"/>
    <dbReference type="OMA" id="YQVIPAS"/>
    <dbReference type="OrthoDB" id="1064531at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046930">
        <property type="term" value="C:pore complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015288">
        <property type="term" value="F:porin activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006811">
        <property type="term" value="P:ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00342">
        <property type="entry name" value="gram_neg_porins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.160.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033900">
        <property type="entry name" value="Gram_neg_porin_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023614">
        <property type="entry name" value="Porin_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002299">
        <property type="entry name" value="Porin_Neis"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13609">
        <property type="entry name" value="Porin_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00184">
        <property type="entry name" value="NEISSPPORIN"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0406">Ion transport</keyword>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-0626">Porin</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1134">Transmembrane beta strand</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193094" evidence="5">
        <location>
            <begin position="25"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="domain" description="Porin_4" evidence="6">
        <location>
            <begin position="12"/>
            <end position="338"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF13609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABF11097.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="379" mass="39667" checksum="6ED0DC24D1D9DBD0" modified="2006-05-30" version="1">MKFKLLAIAATSAAASLAASSAYAQSSVTLYGVADAGVEYLNKAAASNGGVQPGTKGQSLFALQSGNQSGSRWGLRGIEDLGGGLKGIFTLESGFNVDDGKMGQSGRLFGRAAYVGLQNQWGRLTLGRHTTPGFDFGLAFDPMSISSRYSITSQDPWFASRADNSAKYMGTFGGLTVSALYSLNNNNQEVPGNFTNGREYSFSAIYAAGPFSVGAVYDQTNRSQATALSTNKLTRHAAIAGTYAFGQAKVFAGYRYAHAYNGDVLVGTPSHANGSSNLAWAGLGYQVTPALSLAGAAYYQNLRNANAGNPWQFVALADYSLSKRTDLYASMSYALNNGKSTLGVNGFNDQLGSATSSNSEGVQPGANQFGAVVGVRHKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LG75</accession>
    <name>Q1LG75_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Extra-cytoplasmic solute receptor protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">bug</name>
        <name evidence="4" type="ordered locus">Rmet_3983</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="5">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="5">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Periplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the UPF0065 (bug) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10851.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3983"/>
    <dbReference type="EnsemblBacteria" id="ABF10851">
        <property type="protein sequence ID" value="ABF10851"/>
        <property type="gene ID" value="Rmet_3983"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3983"/>
    <dbReference type="eggNOG" id="COG3181">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045683_0_0_4"/>
    <dbReference type="OMA" id="WAQEAPV"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042597">
        <property type="term" value="C:periplasmic space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005064">
        <property type="entry name" value="BUG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042100">
        <property type="entry name" value="Bug_dom1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006311">
        <property type="entry name" value="TAT_signal"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42928">
        <property type="entry name" value="PTHR42928"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03401">
        <property type="entry name" value="TctC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017082">
        <property type="entry name" value="YflP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51318">
        <property type="entry name" value="TAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-0675">Receptor</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193105" evidence="3">
        <location>
            <begin position="30"/>
            <end position="328"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF10851.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="328" mass="34571" checksum="77EA958F88872D4C" modified="2006-05-30" version="1">MFVRMFARRQVVAMLAATASLAAIPAAQANDAYPSKPIRMVVGFPSGGAPDILARIFSEKISLGQPVVVDNKPGAGGNIGAETVARSAPDGYTLALGTVGTHSINGALYSKMPYDMVKDFTPVILLASTPNVLVVHPSVPAKNVQELIALAKAKPGQLTFGTPGIGTSLHVAGELFNTMAGTKIEHVPYKGRAMAIPDLLGGHITMMFDNLPSALPVVKEGKLRALGVTSAQRSTSAPDIPTIAEQGLPGYEATSWFAVFVPAHTPKEIVAKLNAEMNRIFTLPDVQTKLKTLGLDPILGTPEKLAEYQRSEIAKWAKVVKDSGAKAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="70" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LGQ0</accession>
    <name>Q1LGQ0_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_3806</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10676.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008651474.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3806"/>
    <dbReference type="EnsemblBacteria" id="ABF10676">
        <property type="protein sequence ID" value="ABF10676"/>
        <property type="gene ID" value="Rmet_3806"/>
    </dbReference>
    <dbReference type="GeneID" id="60824335"/>
    <dbReference type="KEGG" id="rme:Rmet_3806"/>
    <dbReference type="eggNOG" id="COG1545">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_119412_1_2_4"/>
    <dbReference type="OMA" id="FPPTEHC"/>
    <dbReference type="OrthoDB" id="1756670at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002878">
        <property type="entry name" value="DUF35_OB-fold_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022002">
        <property type="entry name" value="DUF35_Znr_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12172">
        <property type="entry name" value="DUF35_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01796">
        <property type="entry name" value="OB_aCoA_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF35_N" evidence="2">
        <location>
            <begin position="23"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="domain" description="OB_aCoA_assoc" evidence="1">
        <location>
            <begin position="59"/>
            <end position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01796"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF10676.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="132" mass="14702" checksum="837C47BB1649E058" modified="2006-05-30" version="1">MTTPYVPTPYKAPDEQPDNTAFWQAAREGRLLVRQCKSCGKPHWYPRTLCPFCMGDTEWKTASGQGEIYSFSITRRAGPNPFCIAYVKLDDGVTMMTNIVDTDLDTVHIGQRVKVRFAETDGGAPVPVFAPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LH00</accession>
    <name>Q1LH00_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Outer membrane protein (Porin)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Rmet_3704</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="7">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="7">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10576.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518224.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3704"/>
    <dbReference type="EnsemblBacteria" id="ABF10576">
        <property type="protein sequence ID" value="ABF10576"/>
        <property type="gene ID" value="Rmet_3704"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3704"/>
    <dbReference type="eggNOG" id="COG3203">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038238_2_0_4"/>
    <dbReference type="OMA" id="KTACREW"/>
    <dbReference type="OrthoDB" id="1064531at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046930">
        <property type="term" value="C:pore complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015288">
        <property type="term" value="F:porin activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006811">
        <property type="term" value="P:ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00342">
        <property type="entry name" value="gram_neg_porins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.160.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033900">
        <property type="entry name" value="Gram_neg_porin_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023614">
        <property type="entry name" value="Porin_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13609">
        <property type="entry name" value="Porin_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0406">Ion transport</keyword>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-0626">Porin</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1134">Transmembrane beta strand</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193118" evidence="4">
        <location>
            <begin position="21"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="domain" description="Porin_4" evidence="5">
        <location>
            <begin position="7"/>
            <end position="331"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF13609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABF10576.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="363" mass="37998" checksum="FBB00A9A3DA02796" modified="2006-05-30" version="1">MKLRHLALAAALGLPAIANAQSVTLYGVIDTGIEYLNHVGTASNSVVKMANLSGTVPSRWGLRGTEDLGSGMKANFVLESGFAPDSGVSNQGGRLFGRQAWVGLSGNWGQVSLGRQYTMLFWATLDSDILGPNAYGSSSLDNYLPNARADNAIAYKGKFGGFTIGATYSFGRDTVNAGPSPSGTNCGGENPADKTACREWSAMLGYETNWWGILGAYDSQRGGPGAFGGLTSSSLKDDRATLGGYMLVDRAKIGAGWIGRRNGAIATPRSDMFYAGVAYDITPAVTLSGEAFYLKYHNSADKAWLGALRASYAFSKRTSVYTTLGYIDNGGKLALSVDSASTGASPAPGGSQFGAMLGMKHVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="60" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LH53</accession>
    <name>Q1LH53_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Flp/Fap pilin component</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">pilA</name>
        <name evidence="2" type="ordered locus">Rmet_3651</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10523.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518178.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3651"/>
    <dbReference type="EnsemblBacteria" id="ABF10523">
        <property type="protein sequence ID" value="ABF10523"/>
        <property type="gene ID" value="Rmet_3651"/>
    </dbReference>
    <dbReference type="GeneID" id="60826207"/>
    <dbReference type="KEGG" id="rme:Rmet_3651"/>
    <dbReference type="eggNOG" id="COG3847">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_171854_5_1_4"/>
    <dbReference type="OMA" id="ATKVTTW"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007047">
        <property type="entry name" value="Flp_Fap"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04964">
        <property type="entry name" value="Flp_Fap"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="21"/>
            <end position="40"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF10523.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="63" mass="6492" checksum="664FF3952FD18BB3" modified="2006-05-30" version="1">MRQVKASLFRKAQRGATAIEYGLIAGLIAVAIVAGVTNLGQNLGTGFSNLATKVTTWFAAVGP</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="97" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LH75</accession>
    <name>Q1LH75_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Transcriptional regulator, GntR family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Rmet_3629</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="6">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="6">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10501.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518159.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3629"/>
    <dbReference type="EnsemblBacteria" id="ABF10501">
        <property type="protein sequence ID" value="ABF10501"/>
        <property type="gene ID" value="Rmet_3629"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3629"/>
    <dbReference type="eggNOG" id="COG1802">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017584_5_1_4"/>
    <dbReference type="OMA" id="RARQNWD"/>
    <dbReference type="OrthoDB" id="1911275at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07377">
        <property type="entry name" value="WHTH_GntR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.530">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011711">
        <property type="entry name" value="GntR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008920">
        <property type="entry name" value="TF_FadR/GntR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000524">
        <property type="entry name" value="Tscrpt_reg_HTH_GntR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07729">
        <property type="entry name" value="FCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00392">
        <property type="entry name" value="GntR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00035">
        <property type="entry name" value="HTHGNTR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00895">
        <property type="entry name" value="FCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00345">
        <property type="entry name" value="HTH_GNTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48008">
        <property type="entry name" value="SSF48008"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50949">
        <property type="entry name" value="HTH_GNTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0614">Plasmid</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH gntR-type" evidence="4">
        <location>
            <begin position="25"/>
            <end position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABF10501.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="246" mass="26389" checksum="4948138211A39923" modified="2006-05-30" version="1">MNDATASAYSQIPDAPAASLGAQHSPLFALVTDTLRQRILGGQYEPGERLVENTLSLELGVSRIPVREALRALAAEGLVHIEPRRGASVARLSPNIAREMVEVRATLEGLNAKLAAQRRDPALIQELEKVLKEGNDAVDSGQTQRFVELNSRFHEVLGTIAANSVLQEMMRSLRERTALLFAPANLTRARTNWDEHAQILQAVIAGDADLASLLATRHVYSAAKAVEAMNPDATAASASADANKAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LH86</accession>
    <name>Q1LH86_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Organic hydroperoxide resistance protein OhrB, 0smC family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">ohrB</name>
        <name evidence="2" type="ordered locus">Rmet_3618</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the OsmC/Ohr family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10490.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008646921.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3618"/>
    <dbReference type="EnsemblBacteria" id="ABF10490">
        <property type="protein sequence ID" value="ABF10490"/>
        <property type="gene ID" value="Rmet_3618"/>
    </dbReference>
    <dbReference type="GeneID" id="60826175"/>
    <dbReference type="KEGG" id="rme:Rmet_3618"/>
    <dbReference type="eggNOG" id="COG1764">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_106355_2_1_4"/>
    <dbReference type="OMA" id="YSACFDS"/>
    <dbReference type="OrthoDB" id="1984062at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006979">
        <property type="term" value="P:response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015946">
        <property type="entry name" value="KH_dom-like_a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019953">
        <property type="entry name" value="OHR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003718">
        <property type="entry name" value="OsmC/Ohr_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036102">
        <property type="entry name" value="OsmC/Ohrsf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33797">
        <property type="entry name" value="PTHR33797"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02566">
        <property type="entry name" value="OsmC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82784">
        <property type="entry name" value="SSF82784"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03561">
        <property type="entry name" value="organ_hyd_perox"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007378"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF10490.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="140" mass="14510" checksum="11A7CDF82D9E4561" modified="2006-05-30" version="1">MKLEKVLYTAHASATGGRDGRAESSDGQLNVKLAVPKEMGGSGNGMNPEQLFAAGYSACFLGAIRFVAGQQKIAISPDAKIEGAVGIGQIPQGFGIQAELKISLPGLDKEAAQKLVEAAHQVCPYSNATRGNIDVTLIVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="102" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LHC9</accession>
    <name>Q1LHC9_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Sensor signal transduction histidine kinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">Rmet_3575</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10447.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3575"/>
    <dbReference type="EnsemblBacteria" id="ABF10447">
        <property type="protein sequence ID" value="ABF10447"/>
        <property type="gene ID" value="Rmet_3575"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3575"/>
    <dbReference type="eggNOG" id="COG4564">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_20_6_4"/>
    <dbReference type="OMA" id="RNLMHPR"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033480">
        <property type="entry name" value="sCache_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011712">
        <property type="entry name" value="Sig_transdc_His_kin_sub3_dim/P"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017171">
        <property type="entry name" value="Sig_transdc_His_kinase_MctS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07730">
        <property type="entry name" value="HisKA_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17200">
        <property type="entry name" value="sCache_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037314">
        <property type="entry name" value="STHK_MctS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01049">
        <property type="entry name" value="Cache_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="SSF55874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="9" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0808">Transferase</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="214"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="8">
        <location>
            <begin position="265"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="241"/>
            <end position="269"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ABF10447.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="475" mass="52152" checksum="C490CD5FAA98E529" modified="2006-05-30" version="1">MRSSRHRCLPPVQMKLRQKILLLAVAPLAVAMLGIALAVRFQATALVRHERMLVEAAYLQSKETELRHYVELAQSAIAPMVKSGRTDEATRQAAMDALARLDYGRDGYFFLYDLQGRNLMHPRQPELVGQDLWLLRDPEGALTIQKLVAAAKAGGGSVRYLWKKPSSQQVAPKLGYVVAVPAWNWMLGTGIYLDDVEQTLRQLDARAETDIRETMAWIGVIAAISILLVAASGLALNVSEHREADAKLRQLAQRVVQSQEEERARLSRELHDGISQLLVSVKLVLEAATNRIRLAPTEGASVAPILGMALNRLDSVFNEVRRVARNLRPALLDDLGLYAALQHLAREMQAGSTLTINVTQTGKPRSLADEPATALFRIAQEALTNVERHAHATRVDVTLDFADRATRLTVRDNGTGFDVARMQVDPSRGIGLRNLRERVAALGGSFEILSAPTGTLLTASLPTAPQPFSPETSQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LHG0</accession>
    <name>Q1LHG0_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Putative chloramphenicol resistance permease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ordered locus">Rmet_3544</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the EamA transporter family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10416.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517962.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3544"/>
    <dbReference type="EnsemblBacteria" id="ABF10416">
        <property type="protein sequence ID" value="ABF10416"/>
        <property type="gene ID" value="Rmet_3544"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3544"/>
    <dbReference type="eggNOG" id="COG2962">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_054508_1_0_4"/>
    <dbReference type="OMA" id="HRMVWSL"/>
    <dbReference type="OrthoDB" id="1391352at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005887">
        <property type="term" value="C:integral component of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000620">
        <property type="entry name" value="EamA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004626">
        <property type="entry name" value="RarD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22911:SF114">
        <property type="entry name" value="PTHR22911:SF114"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00892">
        <property type="entry name" value="EamA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00688">
        <property type="entry name" value="rarD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="5"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="34"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="67"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="99"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="123"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="146"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="174"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="206"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="239"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="269"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="domain" description="EamA" evidence="9">
        <location>
            <begin position="3"/>
            <end position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007362"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ABF10416.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="304" mass="33111" checksum="4B5F55287F239B51" modified="2006-05-30" version="1">MQLGIFYALLAYIIWGLLPLYIKSLHGVAPLEILLHRMVWSLVFLGAILLWRRHFGWLREVAANPRLVLNFGASAALLCCNWFLYIWAVSADRVVDASLGYFINPLFSVLLGVLLLHERLRPVQWLSIAIAAAGVIWLTVTAGQLPWIALGLAASFGGYGLLRKTGALGALEGLSLETLLLFPFAAAALAFLFLTGQDTTRAAAPGTQVLLVMAGPITAVPLLFFAAGARRIPLSLLGLLQYAGPTLQLALGIWLYHEPFPPQKQIGYALIWVSLAIYAAEGLLVSKLLQRKIISVKNEVSEST</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-10-07" version="38" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LHK2</accession>
    <name>Q1LHK2_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_3502</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10374.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF10374">
        <property type="protein sequence ID" value="ABF10374"/>
        <property type="gene ID" value="Rmet_3502"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3502"/>
    <dbReference type="HOGENOM" id="CLU_2510299_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF10374.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="85" mass="9645" checksum="65B28B1A59C301EE" modified="2006-05-30" version="1">MMAGRILRRNDLKRNWIAPALARMMSDPSATARRSFPSTAFGNRADQRVAVYVSRETDNLPATARNRLPECMEQVRRWLAVRAGA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2007-10-23" modified="2022-05-25" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LI17</accession>
    <name>RL1_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">50S ribosomal protein L1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rplA</name>
        <name type="ordered locus">Rmet_3337</name>
    </gene>
    <organism>
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Binds directly to 23S rRNA. The L1 stalk is quite mobile in the ribosome, and is involved in E site tRNA release.</text>
    </comment>
    <comment type="function">
        <text evidence="1">Protein L1 is also a translational repressor protein, it controls the translation of the L11 operon by binding to its mRNA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the 50S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10209.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517782.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q1LI17"/>
    <dbReference type="SMR" id="Q1LI17"/>
    <dbReference type="STRING" id="266264.Rmet_3337"/>
    <dbReference type="EnsemblBacteria" id="ABF10209">
        <property type="protein sequence ID" value="ABF10209"/>
        <property type="gene ID" value="Rmet_3337"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3337"/>
    <dbReference type="eggNOG" id="COG0081">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_062853_0_0_4"/>
    <dbReference type="OMA" id="GWTDVDV"/>
    <dbReference type="OrthoDB" id="1671455at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015934">
        <property type="term" value="C:large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006417">
        <property type="term" value="P:regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00403">
        <property type="entry name" value="Ribosomal_L1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.790">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01318_B">
        <property type="entry name" value="Ribosomal_L1_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005878">
        <property type="entry name" value="Ribosom_L1_bac-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002143">
        <property type="entry name" value="Ribosomal_L1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023674">
        <property type="entry name" value="Ribosomal_L1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028364">
        <property type="entry name" value="Ribosomal_L1/biogenesis"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016095">
        <property type="entry name" value="Ribosomal_L1_3-a/b-sand"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023673">
        <property type="entry name" value="Ribosomal_L1_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00687">
        <property type="entry name" value="Ribosomal_L1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002155">
        <property type="entry name" value="Ribosomal_L1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56808">
        <property type="entry name" value="SSF56808"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01169">
        <property type="entry name" value="rplA_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01199">
        <property type="entry name" value="RIBOSOMAL_L1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0678">Repressor</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <keyword id="KW-0810">Translation regulation</keyword>
    <keyword id="KW-0820">tRNA-binding</keyword>
    <feature type="chain" id="PRO_0000308082" description="50S ribosomal protein L1">
        <location>
            <begin position="1"/>
            <end position="231"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01318"/>
        </source>
    </evidence>
    <sequence length="231" mass="24072" checksum="C98CBB3AE4CDDC98" modified="2006-05-30" version="1">MAKVSKRVAANKAKIERTKFYPIDEALSLVKSCASAKFDESIDVAVQLGIDAKKSDQVVRGSVVLPAGTGKSVRVAVFAQGEKAEAAKAAGADVVGMEDLAEQVKAGNLDFDIVIASPDTMRIVGTLGQILGPRGLMPNPKVGTVTPDVAQAVKNAKAGQVQFRVDKAGIIHATIGRRSFEDTALKSNLSALLDALVKAKPATSKGVYLRKIAVSSTMGVGVRVEQTSLSA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-12-12" modified="2022-08-03" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LI27</accession>
    <name>RS12_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">30S ribosomal protein S12</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">rpsL</name>
        <name type="ordered locus">Rmet_3327</name>
    </gene>
    <organism>
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">With S4 and S5 plays an important role in translational accuracy.</text>
    </comment>
    <comment type="function">
        <text evidence="2">Interacts with and stabilizes bases of the 16S rRNA that are involved in tRNA selection in the A site and with the mRNA backbone. Located at the interface of the 30S and 50S subunits, it traverses the body of the 30S subunit contacting proteins on the other side and probably holding the rRNA structure together. The combined cluster of proteins S8, S12 and S17 appears to hold together the shoulder and platform of the 30S subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Part of the 30S ribosomal subunit. Contacts proteins S8 and S17. May interact with IF1 in the 30S initiation complex.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the universal ribosomal protein uS12 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10199.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008649178.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q1LI27"/>
    <dbReference type="SMR" id="Q1LI27"/>
    <dbReference type="STRING" id="266264.Rmet_3327"/>
    <dbReference type="PRIDE" id="Q1LI27"/>
    <dbReference type="EnsemblBacteria" id="ABF10199">
        <property type="protein sequence ID" value="ABF10199"/>
        <property type="gene ID" value="Rmet_3327"/>
    </dbReference>
    <dbReference type="GeneID" id="60826590"/>
    <dbReference type="KEGG" id="rme:Rmet_3327"/>
    <dbReference type="eggNOG" id="COG0048">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_104295_1_2_4"/>
    <dbReference type="OMA" id="SPALEKC"/>
    <dbReference type="OrthoDB" id="1707228at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015935">
        <property type="term" value="C:small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd03368">
        <property type="entry name" value="Ribosomal_S12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00403_B">
        <property type="entry name" value="Ribosomal_S12_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006032">
        <property type="entry name" value="Ribosomal_S12/S23"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005679">
        <property type="entry name" value="Ribosomal_S12_bac"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11652">
        <property type="entry name" value="PTHR11652"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00164">
        <property type="entry name" value="Ribosom_S12_S23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002133">
        <property type="entry name" value="Ribosomal_S12/S23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01034">
        <property type="entry name" value="RIBOSOMALS12"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00981">
        <property type="entry name" value="rpsL_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00055">
        <property type="entry name" value="RIBOSOMAL_S12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0488">Methylation</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <keyword id="KW-0820">tRNA-binding</keyword>
    <feature type="chain" id="PRO_0000263577" description="30S ribosomal protein S12">
        <location>
            <begin position="1"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="modified residue" description="3-methylthioaspartic acid" evidence="1">
        <location>
            <position position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00403"/>
        </source>
    </evidence>
    <sequence length="125" mass="13940" checksum="115083226E23387B" modified="2006-05-30" version="1">MPTINQLVRKPRVSEVIKSKSPALENCPQRRGVCTRVYTTTPKKPNSALRKVAKVRLTNGFEVISYIGGEGHNLQEHSVVLIRGGRVKDLPGVRYHIVRGSLDLQGVKDRRQARSKYGAKRPKAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="63" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LIE3</accession>
    <name>Q1LIE3_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_3211</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10083.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517691.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3211"/>
    <dbReference type="EnsemblBacteria" id="ABF10083">
        <property type="protein sequence ID" value="ABF10083"/>
        <property type="gene ID" value="Rmet_3211"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3211"/>
    <dbReference type="HOGENOM" id="CLU_1110676_0_0_4"/>
    <dbReference type="OMA" id="LPDCADK"/>
    <dbReference type="OrthoDB" id="1353781at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193148" evidence="2">
        <location>
            <begin position="22"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="206"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="222"/>
            <end position="241"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF10083.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="250" mass="26014" checksum="7B7CFCBBAA46ABA0" modified="2006-05-30" version="1">MRLPLAGLALASLLATSVAHAAPATPAKPATPAVAAAPAPTGTDALAQILGTLEIGNPLPALPDCADHRTPDGKDVTALCVELRGDGTMRQIGVPRDKRPVFMDGPHLLAFVDGNALVGVMVPTDGIKSQDAAEQSLSARYGKPFRKEMVDMKDKAGNAVKTIHAGWMHRPLTVELYAIPEDPSEGTIELLLPQARALMANRDAAVAKSLAPPASDAAAKPGKGTEKAEKAEKEKAEKKAPVKPVNPGSW</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="72" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LIJ0</accession>
    <name>Q1LIJ0_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Phenylacetate pathway hotdog-fold thioesterase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">paaI</name>
        <name evidence="2" type="ordered locus">Rmet_3164</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10036.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008647561.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3164"/>
    <dbReference type="EnsemblBacteria" id="ABF10036">
        <property type="protein sequence ID" value="ABF10036"/>
        <property type="gene ID" value="Rmet_3164"/>
    </dbReference>
    <dbReference type="GeneID" id="60825457"/>
    <dbReference type="KEGG" id="rme:Rmet_3164"/>
    <dbReference type="eggNOG" id="COG2050">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_089876_11_0_4"/>
    <dbReference type="OMA" id="FACNSHN"/>
    <dbReference type="OrthoDB" id="1814691at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016790">
        <property type="term" value="F:thiolester hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029069">
        <property type="entry name" value="HotDog_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011973">
        <property type="entry name" value="PaaD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003736">
        <property type="entry name" value="PAAI_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006683">
        <property type="entry name" value="Thioestr_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03061">
        <property type="entry name" value="4HBT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54637">
        <property type="entry name" value="SSF54637"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02286">
        <property type="entry name" value="PaaD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00369">
        <property type="entry name" value="unchar_dom_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="4HBT" evidence="1">
        <location>
            <begin position="52"/>
            <end position="124"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF10036.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="146" mass="15637" checksum="2CA20F387B8F9880" modified="2006-05-30" version="1">MTQDPQALAEAAAHAMYESDACSRWLGMVLEAVRPGYARISMPIRPEFLNGHGICHGGLMFTLADSTFAFACNSHNINTVASGCSIEFLRPVHGGDVLTAEAVEQVLSGRHGIYDIRVTNRAGEPVAMFRGKSAQIKGHVVPPAES</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="96" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LIK3</accession>
    <name>Q1LIK3_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Molybdopterin oxidoreductase</fullName>
            <ecNumber evidence="7">1.2.1.2</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Rmet_3151</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the prokaryotic molybdopterin-containing oxidoreductase family.</text>
    </comment>
    <dbReference type="EC" id="1.2.1.2" evidence="7"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10023.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517633.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3151"/>
    <dbReference type="EnsemblBacteria" id="ABF10023">
        <property type="protein sequence ID" value="ABF10023"/>
        <property type="gene ID" value="Rmet_3151"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3151"/>
    <dbReference type="eggNOG" id="COG0243">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000422_13_3_4"/>
    <dbReference type="OMA" id="CPLDCPD"/>
    <dbReference type="OrthoDB" id="88184at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043546">
        <property type="term" value="F:molybdopterin cofactor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02786">
        <property type="entry name" value="MopB_CT_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009010">
        <property type="entry name" value="Asp_de-COase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006657">
        <property type="entry name" value="MoPterin_dinucl-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006656">
        <property type="entry name" value="Mopterin_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006963">
        <property type="entry name" value="Mopterin_OxRdtase_4Fe-4S_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006655">
        <property type="entry name" value="Mopterin_OxRdtase_prok_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037920">
        <property type="entry name" value="YoaE_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04879">
        <property type="entry name" value="Molybdop_Fe4S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00384">
        <property type="entry name" value="Molybdopterin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01568">
        <property type="entry name" value="Molydop_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00926">
        <property type="entry name" value="Molybdop_Fe4S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50692">
        <property type="entry name" value="SSF50692"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51669">
        <property type="entry name" value="4FE4S_MOW_BIS_MGD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00490">
        <property type="entry name" value="MOLYBDOPTERIN_PROK_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="3 5" id="KW-0408">Iron</keyword>
    <keyword evidence="4 5" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="2 5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="4Fe-4S Mo/W bis-MGD-type" evidence="6">
        <location>
            <begin position="3"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="10"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="14"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="18"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="46"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010312"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51669"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABF10023.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="701" mass="76052" checksum="5C7968AB1E27F7ED" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-06-10" modified="2022-08-03" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LIL6</accession>
    <name>COQ7_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">3-demethoxyubiquinol 3-hydroxylase</fullName>
            <shortName evidence="1">DMQ hydroxylase</shortName>
            <ecNumber evidence="1">1.14.99.60</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">2-nonaprenyl-3-methyl-6-methoxy-1,4-benzoquinol hydroxylase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">coq7</name>
        <name type="ordered locus">Rmet_3138</name>
    </gene>
    <organism>
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the hydroxylation of 2-nonaprenyl-3-methyl-6-methoxy-1,4-benzoquinol during ubiquinone biosynthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a 6-methoxy-3-methyl-2-all-trans-polyprenyl-1,4-benzoquinol + AH2 + O2 = A + a 3-demethylubiquinol + H2O</text>
            <dbReference type="Rhea" id="RHEA:50908"/>
            <dbReference type="Rhea" id="RHEA-COMP:10859"/>
            <dbReference type="Rhea" id="RHEA-COMP:10914"/>
            <dbReference type="ChEBI" id="CHEBI:13193"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:17499"/>
            <dbReference type="ChEBI" id="CHEBI:84167"/>
            <dbReference type="ChEBI" id="CHEBI:84422"/>
            <dbReference type="EC" id="1.14.99.60"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </cofactor>
        <text evidence="1">Binds 2 iron ions per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; ubiquinone biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the COQ7 family.</text>
    </comment>
    <dbReference type="EC" id="1.14.99.60" evidence="1"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10010.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008650278.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q1LIL6"/>
    <dbReference type="SMR" id="Q1LIL6"/>
    <dbReference type="STRING" id="266264.Rmet_3138"/>
    <dbReference type="EnsemblBacteria" id="ABF10010">
        <property type="protein sequence ID" value="ABF10010"/>
        <property type="gene ID" value="Rmet_3138"/>
    </dbReference>
    <dbReference type="GeneID" id="60820486"/>
    <dbReference type="KEGG" id="rme:Rmet_3138"/>
    <dbReference type="eggNOG" id="COG2941">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_088601_0_0_4"/>
    <dbReference type="OMA" id="NPLWYGG"/>
    <dbReference type="OrthoDB" id="1382712at2"/>
    <dbReference type="UniPathway" id="UPA00232"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008682">
        <property type="term" value="F:3-demethoxyubiquinol 3-hydroxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006744">
        <property type="term" value="P:ubiquinone biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01042">
        <property type="entry name" value="DMQH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1260.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01658">
        <property type="entry name" value="COQ7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012347">
        <property type="entry name" value="Ferritin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009078">
        <property type="entry name" value="Ferritin-like_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011566">
        <property type="entry name" value="Ubq_synth_Coq7"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11237">
        <property type="entry name" value="PTHR11237"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03232">
        <property type="entry name" value="COQ7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47240">
        <property type="entry name" value="SSF47240"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0408">Iron</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0503">Monooxygenase</keyword>
    <keyword id="KW-0560">Oxidoreductase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0831">Ubiquinone biosynthesis</keyword>
    <feature type="chain" id="PRO_0000338727" description="3-demethoxyubiquinol 3-hydroxylase">
        <location>
            <begin position="1"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="22"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="26"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="56"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="86"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="86"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="89"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="138"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="170"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="170"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="173"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="207" mass="22809" checksum="70339D70AB002512" modified="2006-05-30" version="1">MDTLIREFDVALRAIAGATRAERANPADRLAPETEQMNPEERRHVSGLMRINHVGEVCAQALYQAQKLTARTPAVRAQMDAAAKEEEDHLAWCADRLRELGSRPSLLNPVWYAGAFAIGVLAGRAGDKVSLGFVAETERQVEHHLTSHLDRLPTTDSRSRAILEQMRDDEVRHGDAARNAGGIPLPGPVRALMQVASRVMTTTAYRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LJS1</accession>
    <name>Q1LJS1_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 7">dTDP-4-dehydrorhamnose 3,5-epimerase</fullName>
            <ecNumber evidence="3 7">5.1.3.13</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Thymidine diphospho-4-keto-rhamnose 3,5-epimerase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">rfbC</name>
        <name evidence="8" type="ordered locus">Rmet_2732</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2 7">Catalyzes the epimerization of the C3' and C5'positions of dTDP-6-deoxy-D-xylo-4-hexulose, forming dTDP-6-deoxy-L-lyxo-4-hexulose.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 7">
            <text>dTDP-4-dehydro-6-deoxy-alpha-D-glucose = dTDP-4-dehydro-beta-L-rhamnose</text>
            <dbReference type="Rhea" id="RHEA:16969"/>
            <dbReference type="ChEBI" id="CHEBI:57649"/>
            <dbReference type="ChEBI" id="CHEBI:62830"/>
            <dbReference type="EC" id="5.1.3.13"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="7">Carbohydrate biosynthesis; dTDP-L-rhamnose biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the dTDP-4-dehydrorhamnose 3,5-epimerase family.</text>
    </comment>
    <dbReference type="EC" id="5.1.3.13" evidence="3 7"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09605.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517306.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2732"/>
    <dbReference type="EnsemblBacteria" id="ABF09605">
        <property type="protein sequence ID" value="ABF09605"/>
        <property type="gene ID" value="Rmet_2732"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2732"/>
    <dbReference type="eggNOG" id="COG1898">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_090940_1_1_4"/>
    <dbReference type="OMA" id="RGVFLEW"/>
    <dbReference type="OrthoDB" id="1618609at2"/>
    <dbReference type="UniPathway" id="UPA00124"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008830">
        <property type="term" value="F:dTDP-4-dehydrorhamnose 3,5-epimerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019305">
        <property type="term" value="P:dTDP-rhamnose biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000888">
        <property type="entry name" value="dTDP_sugar_isom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011051">
        <property type="entry name" value="RmlC_Cupin_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21047">
        <property type="entry name" value="PTHR21047"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00908">
        <property type="entry name" value="dTDP_sugar_isom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51182">
        <property type="entry name" value="SSF51182"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01221">
        <property type="entry name" value="rmlC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8" id="KW-0413">Isomerase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Proton acceptor" evidence="5">
        <location>
            <position position="64"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="5">
        <location>
            <position position="133"/>
        </location>
    </feature>
    <feature type="site" description="Participates in a stacking interaction with the thymidine ring of dTDP-4-oxo-6-deoxyglucose" evidence="6">
        <location>
            <position position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001298"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001997"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00019595"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR600888-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR600888-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU364069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ABF09605.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="184" mass="20729" checksum="03B662D1A754D888" modified="2006-05-30" version="1">MSLTVTRTAIPDVMIIEPKVFGDARGFFFESFNQKAFGEATGLDLEFVQDNHSRSAKGVLRGLHYQIQQPQGKLVRVVRGRVFDVAVDIRKQSATFGKWVGVELSEDNHRQFWVPPGFAHGFVVLSESADFLYKTTDYYAPQFERTIAWNDPEIGIEWPQMNVVPLLSAKDQAGVSLSSAELFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LJS9</accession>
    <name>Q1LJS9_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Aminotransferase (DegT/DnrJ/EryC1/StrS-family)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Rmet_2724</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the DegT/DnrJ/EryC1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09597.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517297.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2724"/>
    <dbReference type="EnsemblBacteria" id="ABF09597">
        <property type="protein sequence ID" value="ABF09597"/>
        <property type="gene ID" value="Rmet_2724"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2724"/>
    <dbReference type="eggNOG" id="COG0399">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033332_7_2_4"/>
    <dbReference type="OMA" id="PWPSFTQ"/>
    <dbReference type="OrthoDB" id="1722208at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008483">
        <property type="term" value="F:transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00616">
        <property type="entry name" value="AHBA_syn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000653">
        <property type="entry name" value="DegT/StrS_aminotransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30244">
        <property type="entry name" value="PTHR30244"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01041">
        <property type="entry name" value="DegT_DnrJ_EryC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000390">
        <property type="entry name" value="PLP_StrS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="SSF53383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="2 3" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="active site" description="Proton acceptor" evidence="1">
        <location>
            <position position="185"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="2">
        <location>
            <position position="185"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRSR" id="PIRSR000390-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR000390-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU004508"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF09597.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="391" mass="43387" checksum="61A35544B04661D5" modified="2006-05-30" version="1">MLNTSFSPWPSFTKEEADAVQQVLLSNKVNYWTGTECREFEKEFAAWTGTRHAIALSNGTLALDLALKGLGIGPGDEVIVTPRTFIASASCVVNAGAIPVFADVDPESGNLSAETIARVLTPRTKAVICVHLAGWPCDMDPIMELAARHGLKVIEDCAQAHGARYKGRSVGSIGHVGAWSFCQDKIMTTGGEGGMVTLNDEALWREMWAYKDHGKSFEAVYEREHAPGFRWLHETFGTNWRMLEMQAVIGRIQLRRMTEWTARRTEHAEAIHSACKPFSAVRVPVAPASSTHAQYKCYVYVRPDHLAEGWTRDRIIQTIVDADVPCYQGSCSEVYLEKAFDDTGWRPAERLPIARAMGEDSLMFLVHPTLTKDEIAKTCRVLTDVLGRASR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-07-25" modified="2022-08-03" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LJY1</accession>
    <name>QUEC_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">7-cyano-7-deazaguanine synthase</fullName>
            <ecNumber evidence="1">6.3.4.20</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">7-cyano-7-carbaguanine synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">PreQ(0) synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Queuosine biosynthesis protein QueC</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">queC</name>
        <name type="ordered locus">Rmet_2672</name>
    </gene>
    <organism>
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the ATP-dependent conversion of 7-carboxy-7-deazaguanine (CDG) to 7-cyano-7-deazaguanine (preQ(0)).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>7-carboxy-7-deazaguanine + ATP + NH4(+) = 7-cyano-7-deazaguanine + ADP + H(+) + H2O + phosphate</text>
            <dbReference type="Rhea" id="RHEA:27982"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:45075"/>
            <dbReference type="ChEBI" id="CHEBI:61036"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.4.20"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="1">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Purine metabolism; 7-cyano-7-deazaguanine biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the QueC family.</text>
    </comment>
    <dbReference type="EC" id="6.3.4.20" evidence="1"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09545.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517247.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q1LJY1"/>
    <dbReference type="SMR" id="Q1LJY1"/>
    <dbReference type="STRING" id="266264.Rmet_2672"/>
    <dbReference type="EnsemblBacteria" id="ABF09545">
        <property type="protein sequence ID" value="ABF09545"/>
        <property type="gene ID" value="Rmet_2672"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2672"/>
    <dbReference type="eggNOG" id="COG0603">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_081854_1_1_4"/>
    <dbReference type="OMA" id="CESCMRR"/>
    <dbReference type="OrthoDB" id="1681374at2"/>
    <dbReference type="UniPathway" id="UPA00391"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016879">
        <property type="term" value="F:ligase activity, forming carbon-nitrogen bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008616">
        <property type="term" value="P:queuosine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01995">
        <property type="entry name" value="ExsB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01633">
        <property type="entry name" value="QueC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018317">
        <property type="entry name" value="QueC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42914">
        <property type="entry name" value="PTHR42914"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06508">
        <property type="entry name" value="QueC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006293">
        <property type="entry name" value="ExsB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00364">
        <property type="entry name" value="TIGR00364"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0671">Queuosine biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <feature type="chain" id="PRO_0000246896" description="7-cyano-7-deazaguanine synthase">
        <location>
            <begin position="1"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="9"/>
            <end position="19"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="189"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="199"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="202"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="205"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01633"/>
        </source>
    </evidence>
    <sequence length="227" mass="24079" checksum="7874E3425DA7C2FA" modified="2006-05-30" version="1">MTKRAIVLLSGGLDSATVLAMANAAGFETYALSMRYGQRHSSELEAAKRVAAELGAKRHEIIDLDLRRFGGSALTDDALDVPTDGAAGGIPITYVPARNTIMLSLALGWAEAIGGRDLFFGANAVDYSGYPDCRPEYVAAYETLANLATKAGVEGDRFHVHAPIIDMTKAEIILAGVKLGVDYGLTVSCYKADDDGRACGVCDSCRIRRAGFEAAGVPDPTRYQTPN</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-12-12" modified="2022-05-25" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LK40</accession>
    <name>RS212_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">30S ribosomal protein S21 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpsU2</name>
        <name type="ordered locus">Rmet_2609</name>
    </gene>
    <organism>
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the bacterial ribosomal protein bS21 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09486.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008649856.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q1LK40"/>
    <dbReference type="SMR" id="Q1LK40"/>
    <dbReference type="STRING" id="266264.Rmet_2609"/>
    <dbReference type="PRIDE" id="Q1LK40"/>
    <dbReference type="EnsemblBacteria" id="ABF09486">
        <property type="protein sequence ID" value="ABF09486"/>
        <property type="gene ID" value="Rmet_2609"/>
    </dbReference>
    <dbReference type="GeneID" id="60820954"/>
    <dbReference type="KEGG" id="rme:Rmet_2609"/>
    <dbReference type="eggNOG" id="COG0828">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_159258_1_2_4"/>
    <dbReference type="OMA" id="FKRSCQK"/>
    <dbReference type="OrthoDB" id="2088764at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.1150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00358">
        <property type="entry name" value="Ribosomal_S21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001911">
        <property type="entry name" value="Ribosomal_S21"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018278">
        <property type="entry name" value="Ribosomal_S21_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038380">
        <property type="entry name" value="S21_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21109">
        <property type="entry name" value="PTHR21109"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01165">
        <property type="entry name" value="Ribosomal_S21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00976">
        <property type="entry name" value="RIBOSOMALS21"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00030">
        <property type="entry name" value="S21p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01181">
        <property type="entry name" value="RIBOSOMAL_S21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <feature type="chain" id="PRO_0000266743" description="30S ribosomal protein S21 2">
        <location>
            <begin position="1"/>
            <end position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00358"/>
        </source>
    </evidence>
    <sequence length="70" mass="8608" checksum="7BFD4574EB41928B" modified="2006-05-30" version="1">MTTIRLKENEPFEVALRRFKRTIEKNGLLPELRAREFYEKPTAERKRKRDAAVKRNYKRLRSQMLPKKLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-10-07" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LK91</accession>
    <name>Q1LK91_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_2558</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09435.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2558"/>
    <dbReference type="EnsemblBacteria" id="ABF09435">
        <property type="protein sequence ID" value="ABF09435"/>
        <property type="gene ID" value="Rmet_2558"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2558"/>
    <dbReference type="HOGENOM" id="CLU_2495632_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF09435.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="86" mass="9637" checksum="5DFBDA09091EFA4F" modified="2006-05-30" version="1">MPSESNLAYARRLPQATSTVLRMRFEAELMGQLRAVQNNPALTVKRGRKPSTSVLVRRAVQRYAADISTMDEAELTAEVLAIHRLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LK93</accession>
    <name>Q1LK93_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Transposase IS1087B</fullName>
        </submittedName>
        <submittedName>
            <fullName evidence="2">Transposase catalytic site IS1087B</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">tnpB</name>
        <name evidence="2" type="ordered locus">Rmet_1993</name>
        <name evidence="3" type="ordered locus">Rmet_2556</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="submission" date="2006-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of the chromosome of Ralstonia metallidurans CH34.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Copeland A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Barry K."/>
                <person name="Detter J.C."/>
                <person name="Glavina del Rio T."/>
                <person name="Hammon N."/>
                <person name="Israni S."/>
                <person name="Dalin E."/>
                <person name="Tice H."/>
                <person name="Martinez M."/>
                <person name="Goltsman E."/>
                <person name="Pitluck S."/>
                <person name="Schmutz J."/>
                <person name="Larimer F."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Kim E."/>
                <person name="Mergeay M."/>
                <person name="Benotmane M.A."/>
                <person name="Vallaeys T."/>
                <person name="Michaux A."/>
                <person name="Monchy S."/>
                <person name="Dunn J."/>
                <person name="McCorkle S."/>
                <person name="Taghavi S."/>
                <person name="van der Lelie D."/>
                <person name="Richardson P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CH34</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2010-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Benotmane R."/>
                <person name="Lapidus A."/>
                <person name="McCorkle S."/>
                <person name="Monchy S."/>
                <person name="Taghavi S."/>
                <person name="van der Lelie N."/>
                <person name="Dunn J."/>
                <person name="Leys N."/>
                <person name="Mergeay M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08872.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09433.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1993"/>
    <dbReference type="EnsemblBacteria" id="ABF08872">
        <property type="protein sequence ID" value="ABF08872"/>
        <property type="gene ID" value="Rmet_1993"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF09433">
        <property type="protein sequence ID" value="ABF09433"/>
        <property type="gene ID" value="Rmet_2556"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1993"/>
    <dbReference type="KEGG" id="rme:Rmet_2556"/>
    <dbReference type="eggNOG" id="COG2801">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_052819_0_0_4"/>
    <dbReference type="OMA" id="PRRTMYY"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025948">
        <property type="entry name" value="HTH-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001584">
        <property type="entry name" value="Integrase_cat-core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13276">
        <property type="entry name" value="HTH_21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00665">
        <property type="entry name" value="rve"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="SSF53098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50994">
        <property type="entry name" value="INTEGRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Integrase catalytic" evidence="1">
        <location>
            <begin position="107"/>
            <end position="275"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF08872.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF09433.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="278" mass="31604" checksum="16123973DD356870" modified="2006-05-30" version="1">MKTVCDVLGVARSAVAAKQARSSEWRDGRRARQYDDTALVAEIHELVAGLPTYGYRRVWALLRRSHELSGASPVNAKRVYRVMHDHQLLLRRPGRRLDTRRHDGRVAVDRSNTRWCSDGFEFRCDDGSPLRVTFALDCHDREAISWAATTGGHSGDIVRDVMLAAVEQRFGAVQTEQTIEWLSDNGSAYIDHRTRSFARELGLEPLTTPVRSPQSNGMAESFVKTMKRDYIAFMNKPDVPTALSQLTVAFEQYNDWHPHKALKYRSPREFRRAATSST</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="52" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LKA7</accession>
    <name>Q1LKA7_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_2542</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09419.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517119.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF09419">
        <property type="protein sequence ID" value="ABF09419"/>
        <property type="gene ID" value="Rmet_2542"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2542"/>
    <dbReference type="HOGENOM" id="CLU_134415_0_0_4"/>
    <dbReference type="OMA" id="GMHARVV"/>
    <dbReference type="OrthoDB" id="1679774at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="58"/>
            <end position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF09419.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="174" mass="19981" checksum="553D02256751C7EB" modified="2006-05-30" version="1">MNNELDGVTAWPTTRLGHLQGESDPGRDPGEGVWQNTIAAADHALEEASRIQRGVQQNLKLMQEVRTLRDELRRAHAEIDRYRGMHARVVVSMRQLEDEHSVEMSRLQTDNELLLVRHRVYKLLAEHYATAALRFDPAVFAEHRDRVLEHVLFQRRKGMSLTQIGVGDIAFLLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LKD1</accession>
    <name>Q1LKD1_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Putative chromate transport protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Rmet_2518</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the chromate ion transporter (CHR) (TC 2.A.51) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09395.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517094.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2518"/>
    <dbReference type="EnsemblBacteria" id="ABF09395">
        <property type="protein sequence ID" value="ABF09395"/>
        <property type="gene ID" value="Rmet_2518"/>
    </dbReference>
    <dbReference type="GeneID" id="60821007"/>
    <dbReference type="KEGG" id="rme:Rmet_2518"/>
    <dbReference type="eggNOG" id="COG2059">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018106_3_0_4"/>
    <dbReference type="OMA" id="WIKEEEF"/>
    <dbReference type="OrthoDB" id="1884249at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015109">
        <property type="term" value="F:chromate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003370">
        <property type="entry name" value="Chromate_transpt"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02417">
        <property type="entry name" value="Chromate_transp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="89"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="123"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="154"/>
            <end position="183"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ABF09395.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="193" mass="20533" checksum="9FEAC4E35212CEEF" modified="2006-05-30" version="1">MSSTPIPVESELAPAPTPRTLFIEFARMGLSGFGGVLPFVRRSVVERNRWLNDRDFVEILSLGQVLPGPNVINLALMLGLRFAGLRGALAAFAGLVLMPLVVVLCAMGLYLRFQDVTAVRQMLAGMTAVAAGLVLSTGVKLAQSQPRTVRAIAVGVAAFVAIGLLRWPLLPVMAVLVPVALVLEYRAMRKGGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LKF4</accession>
    <name>Q1LKF4_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Predicted S-adenosylmethionine-dependent methyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_2495</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09372.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2495"/>
    <dbReference type="EnsemblBacteria" id="ABF09372">
        <property type="protein sequence ID" value="ABF09372"/>
        <property type="gene ID" value="Rmet_2495"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2495"/>
    <dbReference type="eggNOG" id="COG0220">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_078981_0_0_4"/>
    <dbReference type="OMA" id="LYPNPWP"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008176">
        <property type="term" value="F:tRNA (guanine-N7-)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003358">
        <property type="entry name" value="tRNA_(Gua-N-7)_MeTrfase_Trmb"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02390">
        <property type="entry name" value="Methyltransf_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51625">
        <property type="entry name" value="SAM_MT_TRMB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF09372.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="236" mass="26450" checksum="3FA3C840596F20F4" modified="2006-05-30" version="1">MPVTIHRCRPSTIAAMFANSRTISSAQSDIHEHLAALVERHLAEPFRKPIGAPSQHAFDAAIDAWQRAGSAPLILDAGCGVGESTLRLATAFPDHYVIGVDQSEKRLTAGKDWWDAPLPENFHWARADLVDFWRLVQSAGVRVTRHYVLYPNPWPKIGHLSRRWHGHAVFPALAACGDYLECRSNWKIYIDEFAAALTMVGRPTQTESWQPEASMTPFERKYAASGHGLWRCVSVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LKT1</accession>
    <name>Q1LKT1_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Alkylhydroperoxidase AhpD core</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_2368</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09245.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004350630.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2368"/>
    <dbReference type="EnsemblBacteria" id="ABF09245">
        <property type="protein sequence ID" value="ABF09245"/>
        <property type="gene ID" value="Rmet_2368"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2368"/>
    <dbReference type="eggNOG" id="COG0599">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_137228_2_0_4"/>
    <dbReference type="OMA" id="NAFTEAC"/>
    <dbReference type="OrthoDB" id="1925754at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004601">
        <property type="term" value="F:peroxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051920">
        <property type="term" value="F:peroxiredoxin activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1290.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029032">
        <property type="entry name" value="AhpD-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004675">
        <property type="entry name" value="AhpD_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003779">
        <property type="entry name" value="CMD-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02627">
        <property type="entry name" value="CMD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69118">
        <property type="entry name" value="SSF69118"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00778">
        <property type="entry name" value="ahpD_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="2" id="KW-0575">Peroxidase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CMD" evidence="1">
        <location>
            <begin position="24"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02627"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF09245.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="125" mass="12568" checksum="3D6C2CAEC1F5B1D0" modified="2006-05-30" version="1">MTTSYRQLTQDVVAHLAPLHKGVPQVMKSFGEMGKAAIADGALDAKTKELIALAIGVAARCDGCIGFHAKALVKLGATAAEVHEALGVAIYMGGGPSAMYASNAVAAFNEFAALSSIPGAPASAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-10-07" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LKZ1</accession>
    <name>Q1LKZ1_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_2306</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09185.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2306"/>
    <dbReference type="EnsemblBacteria" id="ABF09185">
        <property type="protein sequence ID" value="ABF09185"/>
        <property type="gene ID" value="Rmet_2306"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2306"/>
    <dbReference type="eggNOG" id="ENOG5032SWA">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1863431_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF09185.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="137" mass="14945" checksum="D785DB12311DB1D2" modified="2006-05-30" version="1">MSLIRQHHASSQSFQAAADSFRATRQSAIASSLRTRVTRDADAFFSTACGSCHPVRDEAPLFPRSPSMSQQASFGQLALIHCGKFLPLEILHSAAGHYIGTRDTEGPVSRESCQYFRSYAAAQRALERGGWSQLATP</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="52" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LL56</accession>
    <name>Q1LL56_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_2241</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09120.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008651933.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF09120">
        <property type="protein sequence ID" value="ABF09120"/>
        <property type="gene ID" value="Rmet_2241"/>
    </dbReference>
    <dbReference type="GeneID" id="60821186"/>
    <dbReference type="KEGG" id="rme:Rmet_2241"/>
    <dbReference type="HOGENOM" id="CLU_2685191_0_0_4"/>
    <dbReference type="OMA" id="PIYCEIE"/>
    <dbReference type="OrthoDB" id="1923045at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF09120.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="74" mass="7943" checksum="95D40D006C1F2CF0" modified="2006-05-30" version="1">MSKQVFTRAQYLDILNDSLRRHPGFQPGMAFVFLPPGASASQASGVGCTGPMEAMPIYCEIERVASGLIEVEPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="107" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LLA2</accession>
    <name>Q1LLA2_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 6">Carbamoyl-phosphate synthase small chain</fullName>
            <ecNumber evidence="6">6.3.5.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5 6">Carbamoyl-phosphate synthetase glutamine chain</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6 8" type="primary">carA</name>
        <name evidence="8" type="ordered locus">Rmet_2195</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>2 ATP + H2O + hydrogencarbonate + L-glutamine = 2 ADP + carbamoyl phosphate + 2 H(+) + L-glutamate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:18633"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58228"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.5.5"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 6">Amino-acid biosynthesis; L-arginine biosynthesis; carbamoyl phosphate from bicarbonate: step 1/1.</text>
    </comment>
    <comment type="pathway">
        <text evidence="6">Pyrimidine metabolism; UMP biosynthesis via de novo pathway; (S)-dihydroorotate from bicarbonate: step 1/3.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Composed of two chains; the small (or glutamine) chain promotes the hydrolysis of glutamine to ammonia, which is used by the large (or ammonia) chain to synthesize carbamoyl phosphate.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the CarA family.</text>
    </comment>
    <dbReference type="EC" id="6.3.5.5" evidence="6"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09074.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516902.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2195"/>
    <dbReference type="MEROPS" id="C26.954"/>
    <dbReference type="EnsemblBacteria" id="ABF09074">
        <property type="protein sequence ID" value="ABF09074"/>
        <property type="gene ID" value="Rmet_2195"/>
    </dbReference>
    <dbReference type="GeneID" id="60821232"/>
    <dbReference type="KEGG" id="rme:Rmet_2195"/>
    <dbReference type="eggNOG" id="COG0505">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035901_2_1_4"/>
    <dbReference type="OMA" id="CFNTGMT"/>
    <dbReference type="OrthoDB" id="662268at2"/>
    <dbReference type="UniPathway" id="UPA00068">
        <property type="reaction ID" value="UER00171"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00070">
        <property type="reaction ID" value="UER00115"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004088">
        <property type="term" value="F:carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006207">
        <property type="term" value="P:'de novo' pyrimidine nucleobase biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044205">
        <property type="term" value="P:'de novo' UMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006526">
        <property type="term" value="P:arginine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006541">
        <property type="term" value="P:glutamine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01744">
        <property type="entry name" value="GATase1_CPSase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.30.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01209">
        <property type="entry name" value="CPSase_S_chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006274">
        <property type="entry name" value="CarbamoylP_synth_ssu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002474">
        <property type="entry name" value="CarbamoylP_synth_ssu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036480">
        <property type="entry name" value="CarbP_synth_ssu_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035686">
        <property type="entry name" value="CPSase_GATase1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017926">
        <property type="entry name" value="GATASE"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00988">
        <property type="entry name" value="CPSase_sm_chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00117">
        <property type="entry name" value="GATase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01097">
        <property type="entry name" value="CPSase_sm_chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52021">
        <property type="entry name" value="SSF52021"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="SSF52317"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01368">
        <property type="entry name" value="CPSaseIIsmall"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="6" id="KW-0055">Arginine biosynthesis</keyword>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 6 8" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="6 8" id="KW-0436">Ligase</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0665">Pyrimidine biosynthesis</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="CPSase_sm_chain" evidence="7">
        <location>
            <begin position="6"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="region of interest" description="CPSase" evidence="6">
        <location>
            <begin position="1"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="6">
        <location>
            <position position="268"/>
        </location>
    </feature>
    <feature type="active site" evidence="6">
        <location>
            <position position="352"/>
        </location>
    </feature>
    <feature type="active site" evidence="6">
        <location>
            <position position="354"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007800"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00019036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00030197"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM01097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ABF09074.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="382" mass="41103" checksum="E48DB12FB10A16DA" modified="2006-05-30" version="1">MLPSFPSAILALADGTVFRGYSIGASGHTIGEVVFNTAITGYQEILTDPSYSRQIVTLTYPHIGNYGVNPEDVEATKVHAAGLIIKDLPILASNFRKEHTLAHYLKQEKVVAIAGIDTRKLTRILREKGAQNGCILAGEDNVQKAIDLARSFPGLAGMDLAKVVSVKEPYEWTQTEWKLGEGYGKQDKPQFHVVAFDFGVKFNILRMLAERGCKVTVLPAQASAADALALNPDGIFLSNGPGDPEPCDYAIAATREFIARGIPTFGICLGHQIMALAVGAKTLKMSTGHHGANHPVKDHDDGRVVITSQNHGFAVDASTLPANARVTHTSLFDGTLQGFALTDKPAFCFQGHPEASPGPHDIAYLFDRFTKLMQDARAKAAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="113" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LLP8</accession>
    <name>Q1LLP8_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 10">Dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex</fullName>
            <ecNumber evidence="5 10">2.3.1.61</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">2-oxoglutarate dehydrogenase complex component E2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="primary">sucB</name>
        <name evidence="13" type="ordered locus">Rmet_2049</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2 10">E2 component of the 2-oxoglutarate dehydrogenase (OGDH) complex which catalyzes the second step in the conversion of 2-oxoglutarate to succinyl-CoA and CO(2).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 10">
            <text>(R)-N(6)-dihydrolipoyl-L-lysyl-[2-oxoglutarate dehydrogenase complex component E2] + succinyl-CoA = (R)-N(6)-(S(8)-succinyldihydrolipoyl)-L-lysyl-[2-oxoglutarate dehydrogenase complex component E2] + CoA</text>
            <dbReference type="Rhea" id="RHEA:15213"/>
            <dbReference type="Rhea" id="RHEA-COMP:10581"/>
            <dbReference type="Rhea" id="RHEA-COMP:10582"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57292"/>
            <dbReference type="ChEBI" id="CHEBI:83100"/>
            <dbReference type="ChEBI" id="CHEBI:83120"/>
            <dbReference type="EC" id="2.3.1.61"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>(R)-lipoate</name>
            <dbReference type="ChEBI" id="CHEBI:83088"/>
        </cofactor>
        <text evidence="10">Binds 1 lipoyl cofactor covalently.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3 10">Amino-acid degradation; L-lysine degradation via saccharopine pathway; glutaryl-CoA from L-lysine: step 6/6.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 10">Belongs to the 2-oxoacid dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.61" evidence="5 10"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08928.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516760.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2049"/>
    <dbReference type="EnsemblBacteria" id="ABF08928">
        <property type="protein sequence ID" value="ABF08928"/>
        <property type="gene ID" value="Rmet_2049"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2049"/>
    <dbReference type="eggNOG" id="COG0508">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016733_0_0_4"/>
    <dbReference type="OMA" id="MKVPSPG"/>
    <dbReference type="OrthoDB" id="1626282at2"/>
    <dbReference type="UniPathway" id="UPA00868">
        <property type="reaction ID" value="UER00840"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045252">
        <property type="term" value="C:oxoglutarate dehydrogenase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004149">
        <property type="term" value="F:dihydrolipoyllysine-residue succinyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033512">
        <property type="term" value="P:L-lysine catabolic process to acetyl-CoA via saccharopine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.559.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.320.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003016">
        <property type="entry name" value="2-oxoA_DH_lipoyl-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001078">
        <property type="entry name" value="2-oxoacid_DH_actylTfrase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000089">
        <property type="entry name" value="Biotin_lipoyl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023213">
        <property type="entry name" value="CAT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036625">
        <property type="entry name" value="E3-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004167">
        <property type="entry name" value="PSBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011053">
        <property type="entry name" value="Single_hybrid_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006255">
        <property type="entry name" value="SucB"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00198">
        <property type="entry name" value="2-oxoacid_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00364">
        <property type="entry name" value="Biotin_lipoyl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02817">
        <property type="entry name" value="E3_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47005">
        <property type="entry name" value="SSF47005"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51230">
        <property type="entry name" value="SSF51230"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01347">
        <property type="entry name" value="sucB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50968">
        <property type="entry name" value="BIOTINYL_LIPOYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00189">
        <property type="entry name" value="LIPOYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51826">
        <property type="entry name" value="PSBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 13" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="9 10" id="KW-0450">Lipoyl</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 10 13" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 10" id="KW-0816">Tricarboxylic acid cycle</keyword>
    <feature type="domain" description="Lipoyl-binding" evidence="11">
        <location>
            <begin position="3"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="domain" description="Peripheral subunit-binding (PSBD)" evidence="12">
        <location>
            <begin position="116"/>
            <end position="153"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012945"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00019511"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU361138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ABF08928.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="419" mass="43473" checksum="529F56E1CBA31DDE" modified="2006-05-30" version="1">MAIVDVKVPQLSESVAEATMLNWKKKPGEAVAQDEILIEIETDKVVLEVPAPSAGVLSQIIKNDGDTVVADEVIAKIDTAATAGAVAPAAAAPAPAAAAPAPAAAAPAAAASAGAIAMPSAAKLMAEGGLSASQVAGTGKDGRITKGDVLAAGSAPAPAAKAAPAPAAAKPALPQVSAQVDFAALGDRPEERVPMSRLRARIAERLIQSQSTNAILTTFNEVNMKPVMDLRAKYKDRFEKEHGVKLGFMSFFVKAAVHALKKYPVINASVDGNDIVYHGYFDIGIAVGSPRGLVVPILRNADQMSLADIEKKIAEFGTKARDGKLSLEELTGGTFSISNGGTFGSMLSTPIINPPQSAILGVHATKDRAVVEDGQIVIRPMNYLAMSYDHRIIDGREAVLGLVAMKEALEDPARLLLDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-10-07" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LLS4</accession>
    <name>Q1LLS4_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Sterol desaturase family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Rmet_2023</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08902.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2023"/>
    <dbReference type="EnsemblBacteria" id="ABF08902">
        <property type="protein sequence ID" value="ABF08902"/>
        <property type="gene ID" value="Rmet_2023"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2023"/>
    <dbReference type="eggNOG" id="COG3000">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033631_1_1_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008610">
        <property type="term" value="P:lipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006694">
        <property type="entry name" value="Fatty_acid_hydroxylase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04116">
        <property type="entry name" value="FA_hydroxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="34"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="67"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="108"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="158"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="domain" description="Fatty acid hydroxylase" evidence="3">
        <location>
            <begin position="111"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="297"/>
            <end position="322"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF04116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF08902.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="322" mass="37915" checksum="B1FE729384AD7EC3" modified="2006-05-30" version="1">MRMEESLHAKLRKICTLSPMTPQTPAPEAFDPGLILLAFAPVFVLTIGWEAWYWWKRDRSVYSWRDTLSNATLALMHQASDALFLWLMVRTVYTWCYAHGLRAVPETWWSFALLLILQDFLYYWFHRASHQVRWMWASHVAHHSSEGMNFSTAFRQSLTYPISGMWIFWIPLALVGFTPDWVILAVGLNLAFQFFVHTRLGRRWPLVERLVNTPSVHRVHHAKNPQYIDRNYAGVLTIWDRMFGTFVPEEEAPQYGITRQVCTYNPLTLTFHEWRDMFADAWRERDPRYLWKPPEWRSPRSALMPSAGQTPAAEHGQRSDAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-10-07" version="55" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LLT1</accession>
    <name>Q1LLT1_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_2016</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08895.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF08895">
        <property type="protein sequence ID" value="ABF08895"/>
        <property type="gene ID" value="Rmet_2016"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2016"/>
    <dbReference type="eggNOG" id="ENOG502ZRQI">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1229052_0_0_4"/>
    <dbReference type="OMA" id="RHETIDN"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF08895.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="225" mass="24914" checksum="2D68CEF5019A1479" modified="2006-05-30" version="1">MRKLAAARSSVYGHLQVLRYVPSDAVSNSCPAMTKLRYFACAITVLLAATSSNAQQAKPRSGQGAARWAQEPSSFLGIKLGEPMAIKSCRETTDLCLDDGSTNRYAVFSNLPDLGFRYTLTSLIEEGKPISIELKTDHANYSALLSMLKQRFGLPQKAKTEAIQTRMGMKTSSTDSIWHGQKVSMLLNERHETIDNTRVVVMDRKLADELSSRQETEMKSKANAF</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-01-15" modified="2022-08-03" version="100" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LM63</accession>
    <name>CLPX_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">ATP-dependent Clp protease ATP-binding subunit ClpX</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">clpX</name>
        <name type="ordered locus">Rmet_1884</name>
    </gene>
    <organism>
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">ATP-dependent specificity component of the Clp protease. It directs the protease to specific substrates. Can perform chaperone functions in the absence of ClpP.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Component of the ClpX-ClpP complex. Forms a hexameric ring that, in the presence of ATP, binds to fourteen ClpP subunits assembled into a disk-like structure with a central cavity, resembling the structure of eukaryotic proteasomes.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the ClpX chaperone family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08763.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516610.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q1LM63"/>
    <dbReference type="SMR" id="Q1LM63"/>
    <dbReference type="STRING" id="266264.Rmet_1884"/>
    <dbReference type="EnsemblBacteria" id="ABF08763">
        <property type="protein sequence ID" value="ABF08763"/>
        <property type="gene ID" value="Rmet_1884"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1884"/>
    <dbReference type="eggNOG" id="COG1219">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014218_8_2_4"/>
    <dbReference type="OMA" id="HYKRVQA"/>
    <dbReference type="OrthoDB" id="718259at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.20.220.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00175">
        <property type="entry name" value="ClpX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019489">
        <property type="entry name" value="Clp_ATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004487">
        <property type="entry name" value="Clp_protease_ATP-bd_su_ClpX"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046425">
        <property type="entry name" value="ClpX_bact"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010603">
        <property type="entry name" value="Znf_CppX_C4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038366">
        <property type="entry name" value="Znf_CppX_C4_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07724">
        <property type="entry name" value="AAA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10431">
        <property type="entry name" value="ClpB_D2-small"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06689">
        <property type="entry name" value="zf-C4_ClpX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01086">
        <property type="entry name" value="ClpB_D2-small"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00994">
        <property type="entry name" value="zf-C4_ClpX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00382">
        <property type="entry name" value="clpX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51902">
        <property type="entry name" value="CLPX_ZB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0143">Chaperone</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <feature type="chain" id="PRO_1000024629" description="ATP-dependent Clp protease ATP-binding subunit ClpX">
        <location>
            <begin position="1"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="domain" description="ClpX-type ZB" evidence="2">
        <location>
            <begin position="3"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="15"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="18"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="37"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="40"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="123"/>
            <end position="130"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01250"/>
        </source>
    </evidence>
    <sequence length="425" mass="46627" checksum="BB9884E8C5A495FC" modified="2006-05-30" version="1">MADKKGSSSEKLLYCSFCGKSQHEVKKLIAGPSVFICDECIDLCNEIIRDEASATDKDATAATRSDLPTPHEIRESLDQYVIGQDQAKKILAVAVYNHYKRLKHLGKKDDVELSKSNILLIGPTGSGKTLLAQTLARLLNVPFVIADATTLTEAGYVGEDVENIIQKLLQNCNYEVEKAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGTMASVPPQGGRKHPNQDFLQVDTTNILFICGGAFDGLEKVIMQRSDKTGIGFAAQVQSKEEREVSEVLPQTEPEDLIKFGLIPELIGRLPVVATLAKLDEAALMQILIEPKNALVKQYQKLLAMEGVELEIRPAALSAIARKAIRRKTGARGLRSILEQSLMDVMYDLPNYKGVQKVVIDENTITGDAAPLLVYEEQQPKVAGSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-04-07" version="56" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LM78</accession>
    <name>Q1LM78_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_1869</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08748.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516593.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1869"/>
    <dbReference type="EnsemblBacteria" id="ABF08748">
        <property type="protein sequence ID" value="ABF08748"/>
        <property type="gene ID" value="Rmet_1869"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1869"/>
    <dbReference type="HOGENOM" id="CLU_169672_0_0_4"/>
    <dbReference type="OMA" id="WQLWHLS"/>
    <dbReference type="OrthoDB" id="1966465at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="92"/>
            <end position="115"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF08748.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="115" mass="12764" checksum="6F105A181659D2BD" modified="2006-05-30" version="1">MGVTSGYWQMWHIFSSGMVYCALGMPNIECTGGRWRSLLPHLPVRDRYAQAKLLQATVAFMEIRGEVKIPPGSDRLRLLYSRYLPWSARLQTQESAPTPSASPPSPADQPSTAVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LMD4</accession>
    <name>Q1LMD4_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Acyl-CoA transferase/carnitine dehydratase</fullName>
            <ecNumber evidence="1">5.1.99.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_1813</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EC" id="5.1.99.-" evidence="1"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08692.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516542.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1813"/>
    <dbReference type="EnsemblBacteria" id="ABF08692">
        <property type="protein sequence ID" value="ABF08692"/>
        <property type="gene ID" value="Rmet_1813"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1813"/>
    <dbReference type="eggNOG" id="COG1804">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033975_2_0_4"/>
    <dbReference type="OMA" id="ATYLGEF"/>
    <dbReference type="OrthoDB" id="969868at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016853">
        <property type="term" value="F:isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1540.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10540">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003673">
        <property type="entry name" value="CoA-Trfase_fam_III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044855">
        <property type="entry name" value="CoA-Trfase_III_dom3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023606">
        <property type="entry name" value="CoA-Trfase_III_dom_1_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02515">
        <property type="entry name" value="CoA_transf_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89796">
        <property type="entry name" value="SSF89796"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0413">Isomerase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF08692.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="406" mass="43562" checksum="625D99C83EC7CE78" modified="2006-05-30" version="1">MNPEAEQPGGPLAGVRILDMATVIAAPFSSTLCADMGADVVKLELPDGSDPLRGLAPVKGDLALYWKVTNRGKRGITLDVRKPAGRALFLRMLPDFDVLVENFRTGTLDRWGLDIDTLRRANPRLVVLRLTGFGQTGPYAQRAGFARIFEAMSGFTNLTGETGGAPLHMNYPMGDMVAGLFGAFAIASAIAERRRPGNETMPGQEIDLAATEALFRLLEPLAVEYEQLGQVRQRAGNRATYTAPSNMYATADGQWVSLVASSGPIFRRLCQAIGQPGVADDPRFATNPSRVRHLDALDEVVARWFGSHAYADVASTLEAHEIPFSKIFSIADIVDDPHFQERQAIVRLPDPDLGSIPAPCVVPRFAGHTPPPARSGPGVGEHNAEVYGALGLTAADLTGLREGGVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LMG4</accession>
    <name>Q1LMG4_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Phenol hydroxylase P4 protein (Phenol 2-monooxygenase P4 component)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">phyD</name>
        <name evidence="1" type="ordered locus">Rmet_1783</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08662.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008643948.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1783"/>
    <dbReference type="EnsemblBacteria" id="ABF08662">
        <property type="protein sequence ID" value="ABF08662"/>
        <property type="gene ID" value="Rmet_1783"/>
    </dbReference>
    <dbReference type="GeneID" id="60825723"/>
    <dbReference type="KEGG" id="rme:Rmet_1783"/>
    <dbReference type="eggNOG" id="ENOG5032U7S">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2057138_0_0_4"/>
    <dbReference type="OMA" id="LVYFGWD"/>
    <dbReference type="OrthoDB" id="1844114at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018662">
        <property type="term" value="F:phenol 2-monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.560">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006756">
        <property type="entry name" value="Phenol_hydroxylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043010">
        <property type="entry name" value="Phenol_hydroxylase_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04663">
        <property type="entry name" value="Phenol_monoox"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF08662.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="120" mass="13040" checksum="636FDA68138E9FEE" modified="2006-05-30" version="1">MPVIALEPGYTGEVRDRIENFHGNQLVYFGWDQHRLFCSPFTLPLPPDMPFGALVSDVIVPLLGAHPEGARIDWAQVQWLRGDTPFTPDAAASLVDNGLGHKSLLRLRTPGLPGIAGSAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LMJ2</accession>
    <name>Q1LMJ2_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Extra-cytoplasmic solute receptor protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">bug</name>
        <name evidence="4" type="ordered locus">Rmet_1755</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Periplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the UPF0065 (bug) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08634.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516486.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1755"/>
    <dbReference type="EnsemblBacteria" id="ABF08634">
        <property type="protein sequence ID" value="ABF08634"/>
        <property type="gene ID" value="Rmet_1755"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1755"/>
    <dbReference type="eggNOG" id="COG3181">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045683_0_0_4"/>
    <dbReference type="OrthoDB" id="447734at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042597">
        <property type="term" value="C:periplasmic space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005064">
        <property type="entry name" value="BUG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042100">
        <property type="entry name" value="Bug_dom1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006311">
        <property type="entry name" value="TAT_signal"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019546">
        <property type="entry name" value="TAT_signal_bac_arc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42928">
        <property type="entry name" value="PTHR42928"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10518">
        <property type="entry name" value="TAT_signal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03401">
        <property type="entry name" value="TctC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017082">
        <property type="entry name" value="YflP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51318">
        <property type="entry name" value="TAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0675">Receptor</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193323" evidence="3">
        <location>
            <begin position="30"/>
            <end position="332"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF08634.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="332" mass="35060" checksum="CEB3A8ACD9DC6EC3" modified="2006-05-30" version="1">MTKLLISRRQVLKAGAAASMAAVMPSVLAKTGWPDKFIKMVVAFPAGGPTDTAARIVAQKLSGRLGQQVIIENKPGASGSIGTASFIRSPADGYNLSMFGMPALLAPLMYKTNAYDVKKDFLSVATVYVLPMAIVINPAVVPHVETLPDLIRFAKASKTPLSYTSSGTGSFGHLAMEQLKDLGGFDVLHVPYRGSAPAVADLLGGQVGIMFADVVAALPHIRAGKLKAIALSSPNARVLLPGVKTVSEQGFPGFDFDSWGGLIAPLDTPQPIVDRIASETRDIIGKDKELQQKLVSAGAIASFQDGKEMQARLNKDYDRWSAIVKAKGISAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LMT3</accession>
    <name>Q1LMT3_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Transposase (DDE site) ISRme7</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">tnp</name>
        <name evidence="3" type="ordered locus">Rmet_1660</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08543.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1660"/>
    <dbReference type="EnsemblBacteria" id="ABF08543">
        <property type="protein sequence ID" value="ABF08543"/>
        <property type="gene ID" value="Rmet_1660"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1660"/>
    <dbReference type="eggNOG" id="COG3316">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_067322_1_0_4"/>
    <dbReference type="OMA" id="EINHRYD"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032874">
        <property type="entry name" value="DDE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13610">
        <property type="entry name" value="DDE_Tnp_IS240"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="SSF53098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DDE_Tnp_IS240" evidence="2">
        <location>
            <begin position="80"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="232"/>
            <end position="263"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13610"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF08543.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="263" mass="30188" checksum="4A1E9A24534B3C2C" modified="2006-05-30" version="1">MIKELPAGVAKVLKRLHHPLDVILLCVRWYVAYSLSLRDLEEMMAERGLAVDHSTVRRWVIKLLPLFEKAFRRHKQSVSKSWRMDETYLKVRGKWAYLYRAVDKAGNTIDFLLCARRDKVAARRYFEKAIGQNGAPDTVAIDKSAANLAALHAVNAHRETPIRIRQRKYLNNIVEQDHRAIKRRARPMLGFKNFRCARILIGGIETMHMIAKGRCGDPKAFACPPRSNSIPWFHRPAHSSPPTSTNRPYRDRTHQCTDPGCAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LNJ4</accession>
    <name>Q1LNJ4_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">MFS transporter, LplT family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_1399</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08282.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516156.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1399"/>
    <dbReference type="EnsemblBacteria" id="ABF08282">
        <property type="protein sequence ID" value="ABF08282"/>
        <property type="gene ID" value="Rmet_1399"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1399"/>
    <dbReference type="eggNOG" id="COG2814">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047399_0_0_4"/>
    <dbReference type="OMA" id="ICFGFNP"/>
    <dbReference type="OrthoDB" id="961884at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="SSF103473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="42"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="77"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="127"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="172"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="229"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="264"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="290"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="330"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="371"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="398"/>
            <end position="417"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF08282.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="439" mass="47476" checksum="C2C5A91A4A37395A" modified="2006-05-30" version="1">MKKGFYTIMAAQFFSSLADNALLIAAIALLTELHSPQWMTPLLKLFFVLSYVVLAAFVGAFADSMPKGKVMAITNTIKIVGCAIMMFGLHPLLAYGIVGFGAAAYSPAKYGILTELLPPEKLVMANGWIEGLTVGSIILGTVVGGALISVHVSTILLHFDMPYVDTGINTPAEAAMVVIMFFYVIASIFNLFIPDTGARYPQQEKNPVKLIAEFGDCFIALWRDKLGQISLAVTTLFWGVGATLQFIVLKWAEKSLGLNLSQGALLQAVVAVGVAVGAIMAAARIPLRKSLSVLPFGVAMGVTVMIMAFYTKDTIPDVTLDLFGLHMPLYMAIAYVFLMIVGAMSGYFVVPMNALLQHRGHVLLSAGHSIAVQNFNENVSVLLMLCLYALLIKLNVPIGFVIVALGLFICATMLLVMRLHKHNVKIYNSLAMIGEDKHH</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LNP4</accession>
    <name>Q1LNP4_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Conjugal transfer protein TrbG/VirB9/CagX putative exported protein(Protein belongs to CMGI-2)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">trbG</name>
        <name evidence="4" type="ordered locus">Rmet_1349</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TrbG/VirB9 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08232.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516113.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1349"/>
    <dbReference type="EnsemblBacteria" id="ABF08232">
        <property type="protein sequence ID" value="ABF08232"/>
        <property type="gene ID" value="Rmet_1349"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1349"/>
    <dbReference type="eggNOG" id="COG3504">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_058585_1_0_4"/>
    <dbReference type="OMA" id="NDMMIVD"/>
    <dbReference type="OrthoDB" id="1251301at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd06911">
        <property type="entry name" value="VirB9_CagX_TrbG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.2500">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010258">
        <property type="entry name" value="Conjugal_tfr_TrbG/VirB9/CagX"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014142">
        <property type="entry name" value="TrbG_Ti"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033645">
        <property type="entry name" value="VirB9/CagX/TrbG_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038161">
        <property type="entry name" value="VirB9/CagX/TrbG_C_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03524">
        <property type="entry name" value="CagX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02775">
        <property type="entry name" value="TrbG_Ti"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="64"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="70"/>
            <end position="86"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF08232.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="330" mass="35740" checksum="E0C41218BA687ADA" modified="2006-05-30" version="1">MNDLFRKSALPVILLALVGCASQGKPPPTISLDEPVQAQPLPEPPAPVEVVAVPEVLPMPAQLKPLPEAEDAKPAPEPADEKVRVSRANAEARVAPTREGYVNAIQVWPFTDGALYQVYAAVGRVTVVSLQPGEELVTVAAGDTVRWIVGDTSSGSGADLRVNVLVKPIRSGLKTNLVITTSRRTYLLELASTEKTWMASVSWEYPRDRMLALQRQAQAANAAAPVDAGLALENLRFRYAISGSNPPWKPLRAFDDGQKVYIQFPAGIAQGELPPLFVIGPEGDGQLVNYRFRSPYYIVDRLFGAAELRLGGDKGAMVRIERTDGVARRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LNU9</accession>
    <name>Q1LNU9_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">HoxL hydrogenase expression/formation protein hoxL (Belongs to CMGI-2)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">hoxL</name>
        <name evidence="2" type="ordered locus">Rmet_1292</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the HupF/HypC family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08177.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516059.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1292"/>
    <dbReference type="EnsemblBacteria" id="ABF08177">
        <property type="protein sequence ID" value="ABF08177"/>
        <property type="gene ID" value="Rmet_1292"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1292"/>
    <dbReference type="eggNOG" id="COG0298">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2104361_0_0_4"/>
    <dbReference type="OMA" id="EDGAGTC"/>
    <dbReference type="OrthoDB" id="2031925at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019812">
        <property type="entry name" value="Hydgase_assmbl_chp_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001109">
        <property type="entry name" value="Hydrogenase_HupF/HypC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35177">
        <property type="entry name" value="PTHR35177"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01455">
        <property type="entry name" value="HupF_HypC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00445">
        <property type="entry name" value="HUPFHYPC"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00074">
        <property type="entry name" value="hypC_hupF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01097">
        <property type="entry name" value="HUPF_HYPC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0371">Homeobox</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF08177.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="100" mass="10781" checksum="66F6FE2C18004607" modified="2006-05-30" version="1">MCIGIPMQVVEADGRFALCEGRGERRRINTALVGEVSSGDWLLVFLDDARERIDTDRATEVNAALDLVLGAMQGLEAQDDAGFALPSQMSPEQMRALTGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LNV8</accession>
    <name>Q1LNV8_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">HypC1 hydrogenase 2 accessory protein (CMGI-2)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">hypC1</name>
        <name evidence="2" type="ordered locus">Rmet_1283</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the HupF/HypC family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08168.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516050.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1283"/>
    <dbReference type="EnsemblBacteria" id="ABF08168">
        <property type="protein sequence ID" value="ABF08168"/>
        <property type="gene ID" value="Rmet_1283"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1283"/>
    <dbReference type="eggNOG" id="COG0298">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_159381_2_1_4"/>
    <dbReference type="OMA" id="HYPARVD"/>
    <dbReference type="OrthoDB" id="2031925at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019812">
        <property type="entry name" value="Hydgase_assmbl_chp_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001109">
        <property type="entry name" value="Hydrogenase_HupF/HypC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35177">
        <property type="entry name" value="PTHR35177"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01455">
        <property type="entry name" value="HupF_HypC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00445">
        <property type="entry name" value="HUPFHYPC"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00074">
        <property type="entry name" value="hypC_hupF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01097">
        <property type="entry name" value="HUPF_HYPC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF08168.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="75" mass="8008" checksum="933761A3D2CA07CA" modified="2006-05-30" version="1">MCLAIPARLVEKRDHDQAVVDLGGIRKPVSIALVPEAEVGDYVIVHVGHAIGMIDPEEAEKTLALFAEMQAMTGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="61" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LNY2</accession>
    <name>Q1LNY2_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Relaxase type IV secretory pathway VirD2(Protein belongs to CMGI-2)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">virD2</name>
        <name evidence="2" type="ordered locus">Rmet_1259</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08144.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1259"/>
    <dbReference type="EnsemblBacteria" id="ABF08144">
        <property type="protein sequence ID" value="ABF08144"/>
        <property type="gene ID" value="Rmet_1259"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1259"/>
    <dbReference type="eggNOG" id="COG3843">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027492_1_0_4"/>
    <dbReference type="OMA" id="NGDYIAH"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021795">
        <property type="entry name" value="DUF3363"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11843">
        <property type="entry name" value="DUF3363"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="48"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="80"/>
            <end position="94"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF08144.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="701" mass="77334" checksum="0B68D0D13BCA1F6C" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="57" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LNY4</accession>
    <name>Q1LNY4_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_1257</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08142.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516029.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1257"/>
    <dbReference type="EnsemblBacteria" id="ABF08142">
        <property type="protein sequence ID" value="ABF08142"/>
        <property type="gene ID" value="Rmet_1257"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1257"/>
    <dbReference type="eggNOG" id="ENOG502Z7UG">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_123182_0_0_4"/>
    <dbReference type="OMA" id="LKIEGWP"/>
    <dbReference type="OrthoDB" id="1450995at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021263">
        <property type="entry name" value="DUF2840"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11000">
        <property type="entry name" value="DUF2840"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF08142.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="177" mass="19361" checksum="C7F491715CE8A47B" modified="2006-05-30" version="1">MTASASPASGAATAALAAPSGQLASAPLTRVALAYIEPRFKLYLRFGEPARTHQLDRWRRCAVFLPGAMLCRIRWQANDYGTVRWQLMVMQACTPLDAAQRIPGVQPGARLLLHAEGENQVRAVLERIDAIEALGIAPAAVSPAYWRTLANRLAARLPLPEYTAERHAAWLIGRELP</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="103" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LPF2</accession>
    <name>Q1LPF2_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">tRNA(Ile)-lysidine synthase</fullName>
            <ecNumber evidence="7">6.3.4.19</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">tRNA(Ile)-2-lysyl-cytidine synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="7">tRNA(Ile)-lysidine synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7 9" type="primary">tilS</name>
        <name evidence="9" type="ordered locus">Rmet_1088</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionine to isoleucine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 7">
            <text>ATP + cytidine(34) in tRNA(Ile2) + L-lysine = AMP + diphosphate + H(+) + lysidine(34) in tRNA(Ile2)</text>
            <dbReference type="Rhea" id="RHEA:43744"/>
            <dbReference type="Rhea" id="RHEA-COMP:10625"/>
            <dbReference type="Rhea" id="RHEA-COMP:10670"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:32551"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:82748"/>
            <dbReference type="ChEBI" id="CHEBI:83665"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.3.4.19"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 7">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="7">The N-terminal region contains the highly conserved SGGXDS motif, predicted to be a P-loop motif involved in ATP binding.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the tRNA(Ile)-lysidine synthase family.</text>
    </comment>
    <dbReference type="EC" id="6.3.4.19" evidence="7"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07974.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1088"/>
    <dbReference type="EnsemblBacteria" id="ABF07974">
        <property type="protein sequence ID" value="ABF07974"/>
        <property type="gene ID" value="Rmet_1088"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1088"/>
    <dbReference type="eggNOG" id="COG0037">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018869_2_0_4"/>
    <dbReference type="OMA" id="QTETFFL"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016879">
        <property type="term" value="F:ligase activity, forming carbon-nitrogen bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006400">
        <property type="term" value="P:tRNA modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01992">
        <property type="entry name" value="PP-ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01161">
        <property type="entry name" value="tRNA_Ile_lys_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012796">
        <property type="entry name" value="Lysidine-tRNA-synth_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011063">
        <property type="entry name" value="TilS/TtcA_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012094">
        <property type="entry name" value="tRNA_Ile_lys_synt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012795">
        <property type="entry name" value="tRNA_Ile_lys_synt_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015262">
        <property type="entry name" value="tRNA_Ile_lys_synt_subst-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43033">
        <property type="entry name" value="PTHR43033"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01171">
        <property type="entry name" value="ATP_bind_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09179">
        <property type="entry name" value="TilS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11734">
        <property type="entry name" value="TilS_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00977">
        <property type="entry name" value="TilS_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02433">
        <property type="entry name" value="lysidine_TilS_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02432">
        <property type="entry name" value="lysidine_TilS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 7 9" id="KW-0436">Ligase</keyword>
    <keyword evidence="5 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="TilS_C" evidence="8">
        <location>
            <begin position="405"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="55"/>
            <end position="60"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ABF07974.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="487" mass="53060" checksum="0CA1B7BFDB3E47D6" modified="2006-05-30" version="1">MSAGNSTQLIARLTDKVAQALQASATFVVSGDRRLETGSQRAADGQPVTVAVALSGGRDSVALLHATREALAATWPLARLVALHVHHGLQAQADDWDRFCTARCGDLQVPLSSARVTVQAATGEGIEAAARRARHEALGRMCREQGARLLLFAHHRDDQVETVLLRLFRGTGVAGMAGMPARRPLDERGDIQLVRPWLGVTRAEIDDYCAWQGLRWIDDPSNADTRFARNALRAQLPALLQAFPALYENVAQAATHFAQAAGMLDALAAQSLASLVCAGRDADTISELDLAGLRALPSEHADAVLRHWLRDLGTQSPSTARLAAMRAQLIQHQGGEPAIAHDGLVLRRFRDRLLACPAVSAGMPAPFAFEWRGESRIVVPAWRGELHFERDDTFGVPESVLRVPLRLAGRVGGERIVLRPGGPARALKQAYQEADIPAWRRAHLPLLWSADVLVLAAGLGMHRRWPNDAPHAPRWRVEWLARPDLRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="103" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LPM6</accession>
    <name>Q1LPM6_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Endonuclease III</fullName>
            <ecNumber evidence="5">4.2.99.18</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">DNA-(apurinic or apyrimidinic site) lyase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5 7" type="primary">nth</name>
        <name evidence="7" type="ordered locus">Rmet_1014</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">DNA repair enzyme that has both DNA N-glycosylase activity and AP-lyase activity. The DNA N-glycosylase activity releases various damaged pyrimidines from DNA by cleaving the N-glycosidic bond, leaving an AP (apurinic/apyrimidinic) site. The AP-lyase activity cleaves the phosphodiester bond 3' to the AP site by a beta-elimination, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>2'-deoxyribonucleotide-(2'-deoxyribose 5'-phosphate)-2'-deoxyribonucleotide-DNA = a 3'-end 2'-deoxyribonucleotide-(2,3-dehydro-2,3-deoxyribose 5'-phosphate)-DNA + a 5'-end 5'-monophospho-2'-deoxyribonucleoside-DNA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:66592"/>
            <dbReference type="Rhea" id="RHEA-COMP:13180"/>
            <dbReference type="Rhea" id="RHEA-COMP:16897"/>
            <dbReference type="Rhea" id="RHEA-COMP:17067"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:136412"/>
            <dbReference type="ChEBI" id="CHEBI:157695"/>
            <dbReference type="ChEBI" id="CHEBI:167181"/>
            <dbReference type="EC" id="4.2.99.18"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="5">Binds 1 [4Fe-4S] cluster.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the Nth/MutY family.</text>
    </comment>
    <dbReference type="EC" id="4.2.99.18" evidence="5"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07900.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515812.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1014"/>
    <dbReference type="EnsemblBacteria" id="ABF07900">
        <property type="protein sequence ID" value="ABF07900"/>
        <property type="gene ID" value="Rmet_1014"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1014"/>
    <dbReference type="eggNOG" id="COG0177">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012862_3_0_4"/>
    <dbReference type="OMA" id="QIIWYGR"/>
    <dbReference type="OrthoDB" id="1381897at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140078">
        <property type="term" value="F:class I DNA-(apurinic or apyrimidinic site) endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019104">
        <property type="term" value="F:DNA N-glycosylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006284">
        <property type="term" value="P:base-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00056">
        <property type="entry name" value="ENDO3c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1670.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00942">
        <property type="entry name" value="Nth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011257">
        <property type="entry name" value="DNA_glycosylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004036">
        <property type="entry name" value="Endonuclease-III-like_CS2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003265">
        <property type="entry name" value="HhH-GPD_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023170">
        <property type="entry name" value="HhH_base_excis_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000445">
        <property type="entry name" value="HhH_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005759">
        <property type="entry name" value="Nth"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00633">
        <property type="entry name" value="HHH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00730">
        <property type="entry name" value="HhH-GPD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00478">
        <property type="entry name" value="ENDO3c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48150">
        <property type="entry name" value="SSF48150"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01083">
        <property type="entry name" value="nth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01155">
        <property type="entry name" value="ENDONUCLEASE_III_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="1 5" id="KW-0227">DNA damage</keyword>
    <keyword evidence="3 5" id="KW-0234">DNA repair</keyword>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="4 5" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="2 5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0408">Iron</keyword>
    <keyword evidence="5" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="5 7" id="KW-0456">Lyase</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0540">Nuclease</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ENDO3c" evidence="6">
        <location>
            <begin position="38"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="187"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="194"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="197"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="203"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABF07900.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="214" mass="23767" checksum="CAC627EF5DFC343F" modified="2006-05-30" version="1">MNSAKCRAIFETLREVNPTPTTELEYSSPFELLIAVLLSAQATDVGVNKATRLLFPVAHSPQQILALGEAGLIDYIKTIGLYKTKAKHVMETCRILVEKYDGKVPPVREALESLPGVGRKTANVVLNVAFGQPTIAVDTHIFRVSNRTGLAPGKNPDAVEQKLLKVVPKEFLHDAHHWLILHGRYVCKARKPECWHCAIEPLCEFKEKTPAPKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LPR7</accession>
    <name>Q1LPR7_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Acyl-CoA hydratase</fullName>
            <ecNumber evidence="4">4.2.1.17</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">paaG</name>
        <name evidence="4" type="ordered locus">Rmet_0973</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the enoyl-CoA hydratase/isomerase family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.17" evidence="4"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07859.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515777.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0973"/>
    <dbReference type="EnsemblBacteria" id="ABF07859">
        <property type="protein sequence ID" value="ABF07859"/>
        <property type="gene ID" value="Rmet_0973"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0973"/>
    <dbReference type="eggNOG" id="COG1024">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009834_7_2_4"/>
    <dbReference type="OMA" id="SLEGHMQ"/>
    <dbReference type="OrthoDB" id="1275789at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004300">
        <property type="term" value="F:enoyl-CoA hydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.12.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029045">
        <property type="entry name" value="ClpP/crotonase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018376">
        <property type="entry name" value="Enoyl-CoA_hyd/isom_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001753">
        <property type="entry name" value="Enoyl-CoA_hydra/iso"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014748">
        <property type="entry name" value="Enoyl-CoA_hydra_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00378">
        <property type="entry name" value="ECH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52096">
        <property type="entry name" value="SSF52096"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00166">
        <property type="entry name" value="ENOYL_COA_HYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0456">Lyase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF07859.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="259" mass="27317" checksum="AE55061BD7B59A9A" modified="2006-05-30" version="1">MTAQLLSERVDSTLVLTISNPEARNALHPDIYAASAEALEVAARDDSIRAVILTGADGVFCAGGNLNRLLGNRSQPPSVQADSIEVLNQWIESFHAFPKPIIAAVEGPAAGAGFSLVLACDFVVAASDAKFVMAYVKVGLTPDGGGSYEIARMLPRQLASEIMMEGKPVDPARLAHFGIVNRVAAPGQALTEALRIAENLAKESPNAVSGIKSLINHAGTATLTEHLAAERDSFVAALHHKDGGEGISAFLEKRKPNYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="104" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LPU0</accession>
    <name>Q1LPU0_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Cytochrome bo(3) ubiquinol oxidase subunit 3</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Cytochrome o ubiquinol oxidase subunit 3</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">Oxidase bo(3) subunit 3</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="15">Ubiquinol oxidase polypeptide III</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">Ubiquinol oxidase subunit 3</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="primary">cyoC</name>
        <name evidence="19" type="ordered locus">Rmet_0950</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Cytochrome bo(3) ubiquinol terminal oxidase is the component of the aerobic respiratory chain of E.coli that predominates when cells are grown at high aeration. Has proton pump activity across the membrane in addition to electron transfer, pumping 2 protons/electron.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Heterooctamer of two A chains, two B chains, two C chains and two D chains.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="16">Cell membrane</location>
            <topology evidence="16">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 16">Belongs to the cytochrome c oxidase subunit 3 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07836.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008643394.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0950"/>
    <dbReference type="EnsemblBacteria" id="ABF07836">
        <property type="protein sequence ID" value="ABF07836"/>
        <property type="gene ID" value="Rmet_0950"/>
    </dbReference>
    <dbReference type="GeneID" id="60825437"/>
    <dbReference type="KEGG" id="rme:Rmet_0950"/>
    <dbReference type="eggNOG" id="COG1845">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_044071_3_0_4"/>
    <dbReference type="OMA" id="ASGMLWM"/>
    <dbReference type="OrthoDB" id="1502742at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009486">
        <property type="term" value="F:cytochrome bo3 ubiquinol oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004129">
        <property type="term" value="F:cytochrome-c oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019646">
        <property type="term" value="P:aerobic electron transport chain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02863">
        <property type="entry name" value="Ubiquinol_oxidase_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024791">
        <property type="entry name" value="Cyt_c/ubiquinol_Oxase_su3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000298">
        <property type="entry name" value="Cyt_c_oxidase-like_su3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035973">
        <property type="entry name" value="Cyt_c_oxidase_su3-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013833">
        <property type="entry name" value="Cyt_c_oxidase_su3_a-hlx"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014206">
        <property type="entry name" value="Cyt_c_ubiqinol_oxidase_su3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033946">
        <property type="entry name" value="Ubiquinol_oxase_su3_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11403">
        <property type="entry name" value="PTHR11403"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00510">
        <property type="entry name" value="COX3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81452">
        <property type="entry name" value="SSF81452"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02842">
        <property type="entry name" value="CyoC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50253">
        <property type="entry name" value="COX3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-0249">Electron transport</keyword>
    <keyword evidence="10 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="19" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 16 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 17" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="39"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="78"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="108"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="145"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="192"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="domain" description="COX3" evidence="18">
        <location>
            <begin position="37"/>
            <end position="213"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010581"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011700"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00014687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00025694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00030072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00031884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00032189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00032717"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU003376"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50253"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="ABF07836.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="214" mass="23751" checksum="A9DCF64063539C5E" modified="2006-05-30" version="1">MSTEVLDRFGAKAPAHAHSHDEAHDHAHHDTSENTVFGFWLYLMSDCILFACLFAAFAVLRGEVAGGPSGKEIFELNYVLVETAILLFSSITYGFAMVSMQNHQKGRVMFWLGVTFLLGAAFMGMEINEFAHLIHEGAGPSRSAFLSSFFTLVGTHGLHVASGMLWMLVLMWQLSKKGLTPVVAKRLNCLSLFWHFLDVVWIGVFTVVYLMGAM</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="58" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LQ27</accession>
    <name>Q1LQ27_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_0863</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07749.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515690.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0863"/>
    <dbReference type="EnsemblBacteria" id="ABF07749">
        <property type="protein sequence ID" value="ABF07749"/>
        <property type="gene ID" value="Rmet_0863"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0863"/>
    <dbReference type="HOGENOM" id="CLU_2272700_0_0_4"/>
    <dbReference type="OMA" id="NYVLWPN"/>
    <dbReference type="OrthoDB" id="1833229at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF07749.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="96" mass="10924" checksum="7C185F4EBBD9F340" modified="2006-05-30" version="1">MTKSFLAAMRQYVWFKGVKHHVVQGVEALSDLLKSPSAAARAVAAIVAETEQGVQVWVSYFGKPMDPHTNYVLWPNRALRPVRRRATIPEHLLRDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="58" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LQ97</accession>
    <name>Q1LQ97_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">DUF1232 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Rmet_0793</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07679.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008645191.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF07679">
        <property type="protein sequence ID" value="ABF07679"/>
        <property type="gene ID" value="Rmet_0793"/>
    </dbReference>
    <dbReference type="GeneID" id="60825279"/>
    <dbReference type="KEGG" id="rme:Rmet_0793"/>
    <dbReference type="eggNOG" id="COG3339">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_139031_1_1_4"/>
    <dbReference type="OMA" id="MWKRFSA"/>
    <dbReference type="OrthoDB" id="2017850at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010652">
        <property type="entry name" value="DUF1232"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06803">
        <property type="entry name" value="DUF1232"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="DUF1232" evidence="5">
        <location>
            <begin position="35"/>
            <end position="68"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF06803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABF07679.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="97" mass="11030" checksum="B2EEFC422B08477D" modified="2006-05-30" version="1">MWKRFSALWTLVRRDGRLFWFALRHPDSPPWLKPAAAGLLLYAISPIDLIPDFIAGLGIVDDVVLIPLAIHLILKRLPPHILRQAEMRASATTVRSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="116" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LQA2</accession>
    <name>Q1LQA2_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 11">Uracil phosphoribosyltransferase</fullName>
            <ecNumber evidence="3 11">2.4.2.9</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10 11">UMP pyrophosphorylase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">UPRTase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11 12" type="primary">upp</name>
        <name evidence="12" type="ordered locus">Rmet_0788</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Catalyzes the conversion of uracil and 5-phospho-alpha-D-ribose 1-diphosphate (PRPP) to UMP and diphosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>diphosphate + UMP = 5-phospho-alpha-D-ribose 1-diphosphate + uracil</text>
            <dbReference type="Rhea" id="RHEA:13017"/>
            <dbReference type="ChEBI" id="CHEBI:17568"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57865"/>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <dbReference type="EC" id="2.4.2.9"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="11">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="11">Binds 1 Mg(2+) ion per subunit. The magnesium is bound as Mg-PRPP.</text>
    </comment>
    <comment type="activity regulation">
        <text evidence="11">Allosterically activated by GTP.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1 11">Pyrimidine metabolism; UMP biosynthesis via salvage pathway; UMP from uracil: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the UPRTase family.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.9" evidence="3 11"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07674.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008645177.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0788"/>
    <dbReference type="EnsemblBacteria" id="ABF07674">
        <property type="protein sequence ID" value="ABF07674"/>
        <property type="gene ID" value="Rmet_0788"/>
    </dbReference>
    <dbReference type="GeneID" id="60825274"/>
    <dbReference type="KEGG" id="rme:Rmet_0788"/>
    <dbReference type="eggNOG" id="COG0035">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_067096_2_2_4"/>
    <dbReference type="OMA" id="NLFCTPM"/>
    <dbReference type="OrthoDB" id="1581906at2"/>
    <dbReference type="UniPathway" id="UPA00574">
        <property type="reaction ID" value="UER00636"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004845">
        <property type="term" value="F:uracil phosphoribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044206">
        <property type="term" value="P:UMP salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006223">
        <property type="term" value="P:uracil salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06223">
        <property type="entry name" value="PRTases_typeI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2020">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01218_B">
        <property type="entry name" value="Upp_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000836">
        <property type="entry name" value="PRibTrfase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029057">
        <property type="entry name" value="PRTase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034332">
        <property type="entry name" value="Upp_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005765">
        <property type="entry name" value="Ura_phspho_trans"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53271">
        <property type="entry name" value="SSF53271"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01091">
        <property type="entry name" value="upp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 11" id="KW-0021">Allosteric enzyme</keyword>
    <keyword evidence="5 11 12" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="9 11" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="8 11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 11 12" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="5-phospho-alpha-D-ribose 1-diphosphate binding" evidence="11">
        <location>
            <begin position="135"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="region of interest" description="Uracil binding" evidence="11">
        <location>
            <begin position="205"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="85"/>
        </location>
        <ligand>
            <name>5-phospho-alpha-D-ribose 1-diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="110"/>
        </location>
        <ligand>
            <name>5-phospho-alpha-D-ribose 1-diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="200"/>
        </location>
        <ligand>
            <name>uracil</name>
            <dbReference type="ChEBI" id="CHEBI:17568"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="206"/>
        </location>
        <ligand>
            <name>5-phospho-alpha-D-ribose 1-diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00031082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01218"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ABF07674.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="216" mass="24073" checksum="E6310978C40A23E1" modified="2006-05-30" version="1">MKQDPRFPNLFILDHPLIQHKLSHMRDKETSTRTFRELLREITLLMGYEITRNLPLTTRRIDTPLVPLDAPVIAGKKLTIVPVLRAGVGMSDGLVELIPSARIGHIGVYRDDQHRPVEYLVRLPDVEDRSFILCDPMVATGYSAAHAVDVLKRRGVKDESITFVALVAAPEGVEVFQKAHPGVKLYVASLDNHLDDHAYIVPGLGDAGDRLFGTKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LQG1</accession>
    <name>Q1LQG1_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_0729</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07615.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0729"/>
    <dbReference type="EnsemblBacteria" id="ABF07615">
        <property type="protein sequence ID" value="ABF07615"/>
        <property type="gene ID" value="Rmet_0729"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0729"/>
    <dbReference type="eggNOG" id="COG1555">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_052011_4_1_4"/>
    <dbReference type="OMA" id="EGACSRF"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003583">
        <property type="entry name" value="Hlx-hairpin-Hlx_DNA-bd_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010994">
        <property type="entry name" value="RuvA_2-like"/>
    </dbReference>
    <dbReference type="SMART" id="SM00278">
        <property type="entry name" value="HhH1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47781">
        <property type="entry name" value="SSF47781"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193343" evidence="1">
        <location>
            <begin position="43"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="domain" description="HhH1" evidence="2">
        <location>
            <begin position="52"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="domain" description="HhH1" evidence="2">
        <location>
            <begin position="83"/>
            <end position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF07615.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="126" mass="13184" checksum="363C38A6FB8319BF" modified="2006-05-30" version="1">MRRTFDLSLPEISLHRCMRAARRVGAAIGLSMSILAMSPAYADVDVNSADEVALATVKGIGPATAKRIADERGKNGAFKDANDLVDRVPGIGPKSVANLQAAGLSFGKTPDVARKDARPAPKAATR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="113" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LQH9</accession>
    <name>Q1LQH9_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Ubiquinone biosynthesis O-methyltransferase</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">2-polyprenyl-6-hydroxyphenol methylase</fullName>
            <ecNumber evidence="1">2.1.1.222</ecNumber>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">3-demethylubiquinone 3-O-methyltransferase</fullName>
            <ecNumber evidence="1">2.1.1.64</ecNumber>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1 3" type="primary">ubiG</name>
        <name evidence="3" type="ordered locus">Rmet_0711</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">O-methyltransferase that catalyzes the 2 O-methylation steps in the ubiquinone biosynthetic pathway.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a 3-(all-trans-polyprenyl)benzene-1,2-diol + S-adenosyl-L-methionine = a 2-methoxy-6-(all-trans-polyprenyl)phenol + H(+) + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:31411"/>
            <dbReference type="Rhea" id="RHEA-COMP:9550"/>
            <dbReference type="Rhea" id="RHEA-COMP:9551"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:62729"/>
            <dbReference type="ChEBI" id="CHEBI:62731"/>
            <dbReference type="EC" id="2.1.1.222"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a 3-demethylubiquinol + S-adenosyl-L-methionine = a ubiquinol + H(+) + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:44380"/>
            <dbReference type="Rhea" id="RHEA-COMP:9566"/>
            <dbReference type="Rhea" id="RHEA-COMP:10914"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17976"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:84422"/>
            <dbReference type="EC" id="2.1.1.64"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; ubiquinone biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the methyltransferase superfamily. UbiG/COQ3 family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.222" evidence="1"/>
    <dbReference type="EC" id="2.1.1.64" evidence="1"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07597.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515561.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0711"/>
    <dbReference type="EnsemblBacteria" id="ABF07597">
        <property type="protein sequence ID" value="ABF07597"/>
        <property type="gene ID" value="Rmet_0711"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0711"/>
    <dbReference type="eggNOG" id="COG2227">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_042432_5_0_4"/>
    <dbReference type="OMA" id="RGTHDWE"/>
    <dbReference type="OrthoDB" id="1515497at2"/>
    <dbReference type="UniPathway" id="UPA00232"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102208">
        <property type="term" value="F:2-polyprenyl-6-hydroxyphenol methylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008425">
        <property type="term" value="F:2-polyprenyl-6-methoxy-1,4-benzoquinone methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008689">
        <property type="term" value="F:3-demethylubiquinone-9 3-O-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006744">
        <property type="term" value="P:ubiquinone biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00472">
        <property type="entry name" value="UbiG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010233">
        <property type="entry name" value="UbiG_MeTrfase"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01983">
        <property type="entry name" value="UbiG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 3" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="1 3" id="KW-0808">Transferase</keyword>
    <keyword evidence="3" id="KW-0830">Ubiquinone</keyword>
    <keyword evidence="1" id="KW-0831">Ubiquinone biosynthesis</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="52"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="71"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="92"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="136"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF07597.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="247" mass="27366" checksum="AA33BD48C15B6C3C" modified="2006-05-30" version="1">MMSQSTTLHSTPDTPSSRNADPKEIDKFSELAHRWWDPNSEFKPLHDLNPLRLGWIDDIAHLSGKQVIDVGCGGGILSESMARAGATVRGIDLSTKALKVADLHSLESGVAVTYEEIAVEDLAARAPASVDVVTCMEMLEHVPDPQSIVRACMMLTKPGGYVFFSTINRNLKAYLLAVVGAEYVLNMLPRGTHDYEKFITPSELARFARNVGLDMIEMRGMTYNPLTQIYSLGRDTDVNYMMAFRKP</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-01-15" modified="2022-08-03" version="96" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LQJ5</accession>
    <name>SYS_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Serine--tRNA ligase</fullName>
            <ecNumber evidence="1">6.1.1.11</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Seryl-tRNA synthetase</fullName>
            <shortName evidence="1">SerRS</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Seryl-tRNA(Ser/Sec) synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">serS</name>
        <name type="ordered locus">Rmet_0695</name>
    </gene>
    <organism>
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the attachment of serine to tRNA(Ser). Is also able to aminoacylate tRNA(Sec) with serine, to form the misacylated tRNA L-seryl-tRNA(Sec), which will be further converted into selenocysteinyl-tRNA(Sec).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + L-serine + tRNA(Ser) = AMP + diphosphate + H(+) + L-seryl-tRNA(Ser)</text>
            <dbReference type="Rhea" id="RHEA:12292"/>
            <dbReference type="Rhea" id="RHEA-COMP:9669"/>
            <dbReference type="Rhea" id="RHEA-COMP:9703"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78533"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.11"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + L-serine + tRNA(Sec) = AMP + diphosphate + H(+) + L-seryl-tRNA(Sec)</text>
            <dbReference type="Rhea" id="RHEA:42580"/>
            <dbReference type="Rhea" id="RHEA-COMP:9742"/>
            <dbReference type="Rhea" id="RHEA-COMP:10128"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78533"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.11"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Aminoacyl-tRNA biosynthesis; selenocysteinyl-tRNA(Sec) biosynthesis; L-seryl-tRNA(Sec) from L-serine and tRNA(Sec): step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer. The tRNA molecule binds across the dimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="1">Consists of two distinct domains, a catalytic core and a N-terminal extension that is involved in tRNA binding.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-II aminoacyl-tRNA synthetase family. Type-1 seryl-tRNA synthetase subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.11" evidence="1"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07581.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515546.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q1LQJ5"/>
    <dbReference type="SMR" id="Q1LQJ5"/>
    <dbReference type="STRING" id="266264.Rmet_0695"/>
    <dbReference type="EnsemblBacteria" id="ABF07581">
        <property type="protein sequence ID" value="ABF07581"/>
        <property type="gene ID" value="Rmet_0695"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0695"/>
    <dbReference type="eggNOG" id="COG0172">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023797_1_1_4"/>
    <dbReference type="OMA" id="SPCFRRE"/>
    <dbReference type="OrthoDB" id="353391at2"/>
    <dbReference type="UniPathway" id="UPA00906">
        <property type="reaction ID" value="UER00895"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004828">
        <property type="term" value="F:serine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016260">
        <property type="term" value="P:selenocysteine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097056">
        <property type="term" value="P:selenocysteinyl-tRNA(Sec) biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006434">
        <property type="term" value="P:seryl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00770">
        <property type="entry name" value="SerRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00176">
        <property type="entry name" value="Ser_tRNA_synth_type1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002314">
        <property type="entry name" value="aa-tRNA-synt_IIb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002317">
        <property type="entry name" value="Ser-tRNA-ligase_type_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015866">
        <property type="entry name" value="Ser-tRNA-synth_1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042103">
        <property type="entry name" value="SerRS_1_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033729">
        <property type="entry name" value="SerRS_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010978">
        <property type="entry name" value="tRNA-bd_arm"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43697">
        <property type="entry name" value="PTHR43697"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02403">
        <property type="entry name" value="Seryl_tRNA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00587">
        <property type="entry name" value="tRNA-synt_2b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001529">
        <property type="entry name" value="Ser-tRNA-synth_IIa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00981">
        <property type="entry name" value="TRNASYNTHSER"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46589">
        <property type="entry name" value="SSF46589"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="SSF55681"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00414">
        <property type="entry name" value="serS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_1000019788" description="Serine--tRNA ligase">
        <location>
            <begin position="1"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="240"/>
            <end position="242"/>
        </location>
        <ligand>
            <name>L-serine</name>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="271"/>
            <end position="273"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="294"/>
        </location>
        <ligand>
            <name>L-serine</name>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="358"/>
            <end position="361"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="393"/>
        </location>
        <ligand>
            <name>L-serine</name>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00176"/>
        </source>
    </evidence>
    <sequence length="435" mass="48013" checksum="BA5F152592791264" modified="2006-05-30" version="1">MLDIQLLRKDIDAVAQRLATRGFQLDVALFQALEAERKQLQTQTEDLQARRNSLSKQIGMLKGKGEDASGPMAEVAGIGDTLKASAQRLAEIQDQISDAMLSIPNLPHESVPTGKDETENVEVRRVGTPRTFDFEIKDHVDVGARLGLDFETATKVTGSRFAFLRGGVARMHRALVQLMVDTHTLEHGYTEMYVPYIVNAASMRGTGQLPKFEDDLFKVPRKVGGEDGQDAIENFYLIPTAEVPLTNVVRDAIVAAEKLPLRFVAHTPCFRSEAGSYGKDTRGMIRQHQFDKVEMVQVVPSEQSMDALEQMTGHAETILKKLELPFRTVVLCTGDMGFGSTKTYDIEVWIPAQNTYREISSCSSMGDFQARRMQARMRAGQGKPELVHTLNGSGLAVGRTLVAILENYQNADGSVTVPKALQPYMGGITRLEPEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LQL5</accession>
    <name>Q1LQL5_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Alcohol dehydrogenase</fullName>
            <ecNumber evidence="1">1.-.-.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">tsaC</name>
        <name evidence="1" type="ordered locus">Rmet_0675</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EC" id="1.-.-.-" evidence="1"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07561.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515531.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0675"/>
    <dbReference type="EnsemblBacteria" id="ABF07561">
        <property type="protein sequence ID" value="ABF07561"/>
        <property type="gene ID" value="Rmet_0675"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0675"/>
    <dbReference type="eggNOG" id="COG1028">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010194_1_0_4"/>
    <dbReference type="OMA" id="NISSMWG"/>
    <dbReference type="OrthoDB" id="1356861at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF07561.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="253" mass="26526" checksum="380EF8BE663E19A9" modified="2006-05-30" version="1">MARLSGKVAVVTGGGSGFGEGIAHTFAREGASVMVADLNLEAAERVAVAIREAGGQARAVRADVSQGDDVAAMRDATLAAFGDVHCVVNNAGTTHRNKPILEVTEAEFDRVYAVNVKSIYWSAHHFIPHFRKRGGGNFVNVASTAGIRPRPGLVWYNGSKGAVIVASKAMAAELGPDNIRVNCVNPVIGATGLLEQFMGLPDTPENRAKFLATIPMGRMSTPQDVANACLYLASDEAAFITGTCIEVDGGRCV</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LQQ2</accession>
    <name>Q1LQQ2_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Metallophosphoesterase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_0638</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07524.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515495.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0638"/>
    <dbReference type="EnsemblBacteria" id="ABF07524">
        <property type="protein sequence ID" value="ABF07524"/>
        <property type="gene ID" value="Rmet_0638"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0638"/>
    <dbReference type="eggNOG" id="COG1409">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_060372_3_0_4"/>
    <dbReference type="OMA" id="CNPRGYI"/>
    <dbReference type="OrthoDB" id="1690667at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP_ApaH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="SSF56300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Metallophos" evidence="1">
        <location>
            <begin position="2"/>
            <end position="223"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF07524.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="262" mass="28845" checksum="005F908D84BF8C21" modified="2006-05-30" version="1">MKIRILSDLHIEHNMPEDVPACGADLTILAGDIANGRDGIDWAARVFDGPVLYVPGNHEYYESTFDAVSRQMTDAAGESGNVTLLSEGIAEFEDADGQRVRVIGTTWWTDYCLYGVDRVDEAMQACSRVMLDHRLIATADADGQHRPFTPADALARHRAATAWLADALAQPFDGKTVVVTHHGPDLGSLDARYAHDIVSAGFISRRPDLVAQADLWIHGHTHTSFDYWIDSSRVVCNPRGYISRRSGEPENPAFDWSKAVAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="107" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LQV1</accession>
    <name>Q1LQV1_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Histidine kinase</fullName>
            <ecNumber evidence="3">2.7.13.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">gltS2</name>
        <name evidence="10" type="ordered locus">Rmet_0589</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.</text>
            <dbReference type="EC" id="2.7.13.3"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.7.13.3" evidence="3"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07475.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0589"/>
    <dbReference type="EnsemblBacteria" id="ABF07475">
        <property type="protein sequence ID" value="ABF07475"/>
        <property type="gene ID" value="Rmet_0589"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0589"/>
    <dbReference type="eggNOG" id="COG4191">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_94_2_4"/>
    <dbReference type="OMA" id="ATSNWRD"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00082">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033479">
        <property type="entry name" value="dCache_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003661">
        <property type="entry name" value="HisK_dim/P"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036097">
        <property type="entry name" value="HisK_dim/P_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029151">
        <property type="entry name" value="Sensor-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004358">
        <property type="entry name" value="Sig_transdc_His_kin-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017055">
        <property type="entry name" value="Sig_transdc_His_kinase_DctB"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02743">
        <property type="entry name" value="dCache_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036431">
        <property type="entry name" value="STHK_DctB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00344">
        <property type="entry name" value="BCTRLSENSOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00388">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103190">
        <property type="entry name" value="SSF103190"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47384">
        <property type="entry name" value="SSF47384"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="SSF55874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10" id="KW-0418">Kinase</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Histidine kinase" evidence="9">
        <location>
            <begin position="367"/>
            <end position="583"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ABF07475.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="590" mass="63689" checksum="1545261380C7B6B5" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="95" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LQX1</accession>
    <name>Q1LQX1_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Molybdate transporter subunit, ABC-type transporter, ATPase component: MolT family</fullName>
            <ecNumber evidence="9">3.6.3.29</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">modC</name>
        <name evidence="9" type="ordered locus">Rmet_0569</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EC" id="3.6.3.29" evidence="9"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07455.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0569"/>
    <dbReference type="EnsemblBacteria" id="ABF07455">
        <property type="protein sequence ID" value="ABF07455"/>
        <property type="gene ID" value="Rmet_0569"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0569"/>
    <dbReference type="eggNOG" id="COG1118">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_22_4"/>
    <dbReference type="OMA" id="HPPVYAP"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="1" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-1278">Translocase</keyword>
    <feature type="domain" description="ABC transporter" evidence="8">
        <location>
            <begin position="6"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="39"/>
            <end position="46"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ABF07455.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="244" mass="27133" checksum="5F8BA4A6A88074D5" modified="2006-05-30" version="1">MRPAMSMQITIRKRLVSRDRQFALDIGFESDSHRIAMFGPSGSGKTLTLRAIAGLLAPESGRIVLNGRTLFDSESGIDLPPQERRVAYLFQDYALFPHLTVAQNIAFGLNHGWRNPRRRELPPEAAHWVDAFGLREIVNQYPAEISGGQKQRVALARALAPKPDIVLLDEPFSALDPALRSRMREELLSLQANLDVPMVVISHDPQDVEILGDHVLEIREGRIFGSGTGKRHPPVYAPLPARVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LQX3</accession>
    <name>Q1LQX3_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">DNA-binding transcriptional dual regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">modE</name>
        <name evidence="7" type="ordered locus">Rmet_0567</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the ModE family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07453.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515429.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0567"/>
    <dbReference type="EnsemblBacteria" id="ABF07453">
        <property type="protein sequence ID" value="ABF07453"/>
        <property type="gene ID" value="Rmet_0567"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0567"/>
    <dbReference type="eggNOG" id="COG2005">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG3585">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_087839_1_0_4"/>
    <dbReference type="OMA" id="SYKTAWH"/>
    <dbReference type="OrthoDB" id="1582630at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030151">
        <property type="term" value="F:molybdenum ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015689">
        <property type="term" value="P:molybdate ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008995">
        <property type="entry name" value="Mo/tungstate-bd_C_term_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016462">
        <property type="entry name" value="ModE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003725">
        <property type="entry name" value="ModE-bd_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004606">
        <property type="entry name" value="Mop_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005116">
        <property type="entry name" value="Transp-assoc_OB_typ1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000847">
        <property type="entry name" value="Tscrpt_reg_HTH_LysR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00126">
        <property type="entry name" value="HTH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03459">
        <property type="entry name" value="TOBE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005763">
        <property type="entry name" value="Txn_reg_ModE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50331">
        <property type="entry name" value="SSF50331"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00637">
        <property type="entry name" value="ModE_repress"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00638">
        <property type="entry name" value="Mop"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51866">
        <property type="entry name" value="MOP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2 4 5" id="KW-0500">Molybdenum</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Mop" evidence="6">
        <location>
            <begin position="128"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="domain" description="Mop" evidence="6">
        <location>
            <begin position="200"/>
            <end position="266"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022505"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRNR" id="PIRNR005763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01213"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51866"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABF07453.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="268" mass="27803" checksum="F605BBA05231F45A" modified="2006-05-30" version="1">MLELQGAIWFRAGTHDWGGKDRIALLAAIGEHGSITAAARAVGISYKAAWDAIDAMNNSAGEPLVSRAAGGKGGGGTRLTERAERLIRTYHAMEAEHARFVAYLDKVGSALSDADADDLHLMRRFMVQTSARNKLFGRVEAVRGGAVNDEVTLALPGGQRVVSTITHESVETLGLAAGAEAFALVKASSVMIGLPDPNVRLSARNQLPGVVSRVQPGAVNAEVVLDLDGGGTVAAIITNAAVTELDLKVGTRAVAIFKASSVILGTVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LR03</accession>
    <name>Q1LR03_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Biopolymer transport channel protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">exbB2</name>
        <name evidence="9" type="ordered locus">Rmet_0537</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Membrane</location>
            <topology evidence="1 6">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the exbB/tolQ family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07423.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515398.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0537"/>
    <dbReference type="EnsemblBacteria" id="ABF07423">
        <property type="protein sequence ID" value="ABF07423"/>
        <property type="gene ID" value="Rmet_0537"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0537"/>
    <dbReference type="eggNOG" id="COG0811">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_053325_4_5_4"/>
    <dbReference type="OMA" id="LEKNVSW"/>
    <dbReference type="OrthoDB" id="1796180at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002898">
        <property type="entry name" value="MotA_ExbB_proton_chnl"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01618">
        <property type="entry name" value="MotA_ExbB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0653">Protein transport</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="110"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="154"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="domain" description="MotA_ExbB" evidence="8">
        <location>
            <begin position="66"/>
            <end position="190"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU004057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ABF07423.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="206" mass="22607" checksum="EFDC5C207920FD4A" modified="2006-05-30" version="1">MFSIIQAAGWPIWPLLLASVIALALIVERFISLKRSKILPPKLYEEALSAAQQRRATPEVVNNLEQNSPLGRVLAAGLRHVVLQPQTSRDAAKDVVEEAGRVVAHELERYLNALGTIASVAPLMGLLGTVIGMIEIFGNQGGTGANPEQLAHGISVALYNTAFGLIVAIPALIFWRYFRRRVDDYVAELEFRATSFLDAILPQRRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="111" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LR76</accession>
    <name>Q1LR76_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Sensor protein QseC</fullName>
            <ecNumber evidence="4">2.7.13.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ordered locus">Rmet_0464</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.</text>
            <dbReference type="EC" id="2.7.13.3"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell inner membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.7.13.3" evidence="4"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07350.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515339.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0464"/>
    <dbReference type="EnsemblBacteria" id="ABF07350">
        <property type="protein sequence ID" value="ABF07350"/>
        <property type="gene ID" value="Rmet_0464"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0464"/>
    <dbReference type="eggNOG" id="COG0642">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_89_37_4"/>
    <dbReference type="OMA" id="REIVQQH"/>
    <dbReference type="OrthoDB" id="692375at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00082">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013727">
        <property type="entry name" value="2CSK_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003660">
        <property type="entry name" value="HAMP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003661">
        <property type="entry name" value="HisK_dim/P"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036097">
        <property type="entry name" value="HisK_dim/P_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004358">
        <property type="entry name" value="Sig_transdc_His_kin-like_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08521">
        <property type="entry name" value="2CSK_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00512">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00344">
        <property type="entry name" value="BCTRLSENSOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00388">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47384">
        <property type="entry name" value="SSF47384"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="SSF55874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50885">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9 15" id="KW-0418">Kinase</keyword>
    <keyword evidence="11 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <keyword evidence="8 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="63"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="213"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="domain" description="HAMP" evidence="14">
        <location>
            <begin position="237"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="13">
        <location>
            <begin position="297"/>
            <end position="518"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004429"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00017234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="ABF07350.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="524" mass="57314" checksum="C0C9A0ECAFD8DA32" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LRH6</accession>
    <name>Q1LRH6_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative carbon-monoxide dehydrogenase, large subunt</fullName>
            <ecNumber evidence="2">1.2.99.2</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">coxL</name>
        <name evidence="2" type="ordered locus">Rmet_0364</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EC" id="1.2.99.2" evidence="2"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07250.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515242.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0364"/>
    <dbReference type="EnsemblBacteria" id="ABF07250">
        <property type="protein sequence ID" value="ABF07250"/>
        <property type="gene ID" value="Rmet_0364"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0364"/>
    <dbReference type="eggNOG" id="COG1529">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001681_2_0_4"/>
    <dbReference type="OMA" id="CTHNPLG"/>
    <dbReference type="OrthoDB" id="117507at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000674">
        <property type="entry name" value="Ald_Oxase/Xan_DH_a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036856">
        <property type="entry name" value="Ald_Oxase/Xan_DH_a/b_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016208">
        <property type="entry name" value="Ald_Oxase/xanthine_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008274">
        <property type="entry name" value="AldOxase/xan_DH_Mopterin-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037165">
        <property type="entry name" value="AldOxase/xan_DH_Mopterin-bd_sf"/>
    </dbReference>
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        <property type="entry name" value="PTHR11908"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Ald_Xan_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02738">
        <property type="entry name" value="Ald_Xan_dh_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01008">
        <property type="entry name" value="Ald_Xan_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54665">
        <property type="entry name" value="SSF54665"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56003">
        <property type="entry name" value="SSF56003"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ald_Xan_dh_C" evidence="1">
        <location>
            <begin position="22"/>
            <end position="142"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM01008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF07250.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="792" mass="84410" checksum="C94BCF6E1D02F8AD" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LRX4</accession>
    <name>Q1LRX4_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">ABC-type transporter, livH-like permease component</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Rmet_0216</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07102.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515121.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0216"/>
    <dbReference type="EnsemblBacteria" id="ABF07102">
        <property type="protein sequence ID" value="ABF07102"/>
        <property type="gene ID" value="Rmet_0216"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0216"/>
    <dbReference type="eggNOG" id="COG0559">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039929_1_1_4"/>
    <dbReference type="OMA" id="VAGIMWG"/>
    <dbReference type="OrthoDB" id="1529037at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001851">
        <property type="entry name" value="ABC_transp_permease"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02653">
        <property type="entry name" value="BPD_transp_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="7"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="34"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="60"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="92"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="145"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="191"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="230"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="272"/>
            <end position="290"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABF07102.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="304" mass="32490" checksum="EA7A043ABD6F2449" modified="2006-05-30" version="1">MSFFFEILIGGLLSGLMYSLVALGFVLIYKASGVFNFAQGAMVYFAALAVVGLMDKGLPMWAAVIGAFIVMIIVGMSTERFVLRKLVNQPPITLFMATIGLSFFLEGLGPLLFGNEVRPIQLGIVDEPIESILTRFNIVVSKFDLAAALIAAVLVAGLALFFQYTKVGRALRAVADDHQAALSLGIPLQNIWAIVWGVAGFVALVAGMLWGSRNGVQFALTLTALKALPVLILGGFTSVPGAIVGGLIIGASEKLAEIYIPPVFQSVFGGNFGGIEGWFPYVFALLFLLVRPEGLFGEKHIDRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="60" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LS33</accession>
    <name>Q1LS33_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_0157</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07043.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515069.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0157"/>
    <dbReference type="EnsemblBacteria" id="ABF07043">
        <property type="protein sequence ID" value="ABF07043"/>
        <property type="gene ID" value="Rmet_0157"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0157"/>
    <dbReference type="eggNOG" id="ENOG502Z7Z3">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_083861_0_0_4"/>
    <dbReference type="OMA" id="WRKDDTR"/>
    <dbReference type="OrthoDB" id="847210at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021266">
        <property type="entry name" value="Kdo_hydroxlase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11004">
        <property type="entry name" value="Kdo_hydroxy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF07043.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="297" mass="33827" checksum="C1A72506D0D3AC2A" modified="2006-05-30" version="1">MAMSLDLIRTQPYTDWSPEVSPETQATLRRELEQGAVLYFPNLHFRFAPGEERFLDSRYSDGKSKNINLRANEGAVRGAAGTQQDLTDLYNLIRRYAESTESLIHALFPEYGPHMTRAGTSLRPSEIAGRPVSWRKDDTRLHVDAFPSNPLLGKRLLRVFHNIDPEAPRVWRVGEPFADFAGRFVPKTHGMWPGQSWLMKTLHITKRARSEYDHRMLQLHDLAKADLDYQKSVPQQEFQFPPGSTWIVFSDQLLHAAMRGKAMMEQTIYLDPAAIGDRTHSPEAVLSKMLGKPMLAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="98" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LS41</accession>
    <name>Q1LS41_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Exodeoxyribonuclease III</fullName>
            <ecNumber evidence="3">3.1.11.2</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">xthA1</name>
        <name evidence="3" type="ordered locus">Rmet_0149</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the DNA repair enzymes AP/ExoA family.</text>
    </comment>
    <dbReference type="EC" id="3.1.11.2" evidence="3"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07035.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515063.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0149"/>
    <dbReference type="EnsemblBacteria" id="ABF07035">
        <property type="protein sequence ID" value="ABF07035"/>
        <property type="gene ID" value="Rmet_0149"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0149"/>
    <dbReference type="eggNOG" id="COG0708">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027539_3_0_4"/>
    <dbReference type="OMA" id="DCICVQE"/>
    <dbReference type="OrthoDB" id="1893707at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008853">
        <property type="term" value="F:exodeoxyribonuclease III activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004808">
        <property type="entry name" value="AP_endonuc_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020847">
        <property type="entry name" value="AP_endonuclease_F1_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005135">
        <property type="entry name" value="Endo/exonuclease/phosphatase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22748">
        <property type="entry name" value="PTHR22748"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03372">
        <property type="entry name" value="Exo_endo_phos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="SSF56219"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00633">
        <property type="entry name" value="xth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00726">
        <property type="entry name" value="AP_NUCLEASE_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51435">
        <property type="entry name" value="AP_NUCLEASE_F1_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Endo/exonuclease/phosphatase" evidence="2">
        <location>
            <begin position="6"/>
            <end position="247"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007092"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF03372"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF07035.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="258" mass="29537" checksum="F2F2E5A031172457" modified="2006-05-30" version="1">MLRIISANLNGIRSASKKGFFEWMGKQEADMVCVQELKAQEADMTEAFLAPHGYHGYFHYAEKKGYSGVGLYTRHKPERVIIGFGNTEFDAEGRYVEVQYPHLAVISVYVPSGSSSEERQQAKFRFMEAFLPHLLELKASGREIVLCGDVNIAHKEIDIKNWKGNLKNSGFLPEERAWIGELFDNHGYVDVFRQLDPRPEQYTWWSNRGQAYAKNVGWRIDYHLTTAKIAATAKATSIYKDEKFSDHAPLSIDYDFPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="99" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LS42</accession>
    <name>Q1LS42_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">N-acetyl-gamma-glutamyl-phosphate reductase</fullName>
            <shortName evidence="1">AGPR</shortName>
            <ecNumber evidence="1">1.2.1.38</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">N-acetyl-glutamate semialdehyde dehydrogenase</fullName>
            <shortName evidence="1">NAGSA dehydrogenase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="3" type="primary">argC1</name>
        <name evidence="1" type="synonym">argC</name>
        <name evidence="3" type="ordered locus">Rmet_0148</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the NADPH-dependent reduction of N-acetyl-5-glutamyl phosphate to yield N-acetyl-L-glutamate 5-semialdehyde.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>N-acetyl-L-glutamate 5-semialdehyde + NADP(+) + phosphate = H(+) + N-acetyl-L-glutamyl 5-phosphate + NADPH</text>
            <dbReference type="Rhea" id="RHEA:21588"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29123"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:57936"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.2.1.38"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-arginine biosynthesis; N(2)-acetyl-L-ornithine from L-glutamate: step 3/4.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the NAGSA dehydrogenase family. Type 2 subfamily.</text>
    </comment>
    <dbReference type="EC" id="1.2.1.38" evidence="1"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07034.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0148"/>
    <dbReference type="EnsemblBacteria" id="ABF07034">
        <property type="protein sequence ID" value="ABF07034"/>
        <property type="gene ID" value="Rmet_0148"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0148"/>
    <dbReference type="eggNOG" id="COG0002">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_077118_0_0_4"/>
    <dbReference type="OMA" id="PHLTPMI"/>
    <dbReference type="UniPathway" id="UPA00068">
        <property type="reaction ID" value="UER00108"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003942">
        <property type="term" value="F:N-acetyl-gamma-glutamyl-phosphate reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006526">
        <property type="term" value="P:arginine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01110">
        <property type="entry name" value="ArgC_type2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010136">
        <property type="entry name" value="AGPR_type-2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000534">
        <property type="entry name" value="Semialdehyde_DH_NAD-bd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01118">
        <property type="entry name" value="Semialdhyde_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00859">
        <property type="entry name" value="Semialdhyde_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01851">
        <property type="entry name" value="argC_other"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="1" id="KW-0055">Arginine biosynthesis</keyword>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="1" id="KW-0521">NADP</keyword>
    <keyword evidence="1 3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Semialdhyde_dh" evidence="2">
        <location>
            <begin position="24"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="136"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF07034.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="335" mass="36460" checksum="97DC9155601B28B7" modified="2006-05-30" version="1">MLVLRPQGRETFRIFQSESAMVFKVFVDGQEGTTGLRLLDYLSGRTDVELLRIADDKRKDPQERARFLNASDVSFLCLPDVASREAVSLVTNPDTCVIDASTAFRTADNWAYGLPELTRGQREKVRSSKRIAVPGCHASAFVLAVRPLVEAGLMPADYPASAFSLTGYSGGGKSMIAEFEAGGNPKLMSPRPYALGLAHKHLPEMRVQTGLSQAPIFNPIVGNFLKGLAVTVPVYPGLLSRKADAEAILDIYRKHYEGEQFVRVHPFNSDANLDNGFFDVQANNDTNRVDLFVFGNEERLNLVARLDNLGKGAAGAAVQCMNVHIGADEATGLVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-03-23" modified="2020-10-07" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>D3DXL2</accession>
    <name>D3DXL2_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_6414</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ADC45032.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADC45032">
        <property type="protein sequence ID" value="ADC45032"/>
        <property type="gene ID" value="Rmet_6414"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6414"/>
    <dbReference type="HOGENOM" id="CLU_2772941_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADC45032.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="69" mass="7967" checksum="1DCC67E1E9C937B1" modified="2010-03-23" version="1">MRELASGHAIPHEILRVSSNAMPARPRGFLSNSLFNKECEAFDMIYSQFHIAVSHFALQIFLQRLCRYV</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-03-23" modified="2020-10-07" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>D3DXT2</accession>
    <name>D3DXT2_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_6494</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ADC45102.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADC45102">
        <property type="protein sequence ID" value="ADC45102"/>
        <property type="gene ID" value="Rmet_6494"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6494"/>
    <dbReference type="HOGENOM" id="CLU_2993479_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADC45102.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="57" mass="6780" checksum="BE1C759673282611" modified="2010-03-23" version="1">MHRTCRWAEVCAESLSIACQRAYKTLSFAVENLSMRRIEYIRGDFREMALRRAGRLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-03-23" modified="2020-10-07" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>D3DY30</accession>
    <name>D3DY30_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_6599</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ADC45200.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADC45200">
        <property type="protein sequence ID" value="ADC45200"/>
        <property type="gene ID" value="Rmet_6599"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6599"/>
    <dbReference type="HOGENOM" id="CLU_3426662_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADC45200.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="21" mass="2251" checksum="938105DCB99D9EAA" modified="2010-03-23" version="1">MMLTGGVGLSPMAWPPLCIYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-03-23" modified="2020-10-07" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>D3DY68</accession>
    <name>D3DY68_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_6638</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ADC45238.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADC45238">
        <property type="protein sequence ID" value="ADC45238"/>
        <property type="gene ID" value="Rmet_6638"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6638"/>
    <dbReference type="HOGENOM" id="CLU_3011048_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADC45238.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="56" mass="6456" checksum="F8AC05146E1766BC" modified="2010-03-23" version="1">MLKLFGKCDENLGKCKRFQFPAQRRNAPGTALKWLGRYSTLRRKRLPPKRGIPPTG</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-03-23" modified="2020-10-07" version="27" xmlns="http://uniprot.org/uniprot">
    <accession>D3DY79</accession>
    <name>D3DY79_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_6650</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ADC45249.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_6650"/>
    <dbReference type="EnsemblBacteria" id="ADC45249">
        <property type="protein sequence ID" value="ADC45249"/>
        <property type="gene ID" value="Rmet_6650"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6650"/>
    <dbReference type="HOGENOM" id="CLU_2094831_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADC45249.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="116" mass="13081" checksum="8766534008913814" modified="2010-03-23" version="1">MVTDTDDKTLPSPCHSGERAFVYLQEFAGVFKEGYALRRQRHIPGGPLDQPTIEPLFEPLQLQADSGLRRPHGFSRARKAAKVRNAHESLDGIQVERVPFHFQLLLLILGHHGFSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-03-23" modified="2020-10-07" version="26" xmlns="http://uniprot.org/uniprot">
    <accession>D3DYA9</accession>
    <name>D3DYA9_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_6696</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ADC45279.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADC45279">
        <property type="protein sequence ID" value="ADC45279"/>
        <property type="gene ID" value="Rmet_6696"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6696"/>
    <dbReference type="HOGENOM" id="CLU_3295405_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADC45279.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="40" mass="4385" checksum="E591345BA4A7F446" modified="2010-03-23" version="1">MADCLRPHPEPGPLCFILLVSLRQEWEGSAAPHVKPCAKS</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1998-07-15" modified="2022-08-03" version="132" xmlns="http://uniprot.org/uniprot">
    <accession>O33522</accession>
    <accession>Q1LJ81</accession>
    <name>DNAK_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName>Chaperone protein DnaK</fullName>
        </recommendedName>
        <alternativeName>
            <fullName>HSP70</fullName>
        </alternativeName>
        <alternativeName>
            <fullName>Heat shock 70 kDa protein</fullName>
        </alternativeName>
        <alternativeName>
            <fullName>Heat shock protein 70</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">dnaK</name>
        <name type="ordered locus">Rmet_2922</name>
    </gene>
    <organism>
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="1997-10" db="EMBL/GenBank/DDBJ databases">
            <title>Construction and characterization of a DnaK mutant of Ralstonia eutropha strain CH34.</title>
            <authorList>
                <person name="Talbi S."/>
                <person name="van der Lelie D."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC DNA]</scope>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Acts as a chaperone.</text>
    </comment>
    <comment type="induction">
        <text evidence="1">By stress conditions e.g. heat shock.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the heat shock protein 70 family.</text>
    </comment>
    <dbReference type="EMBL" id="AJ001727">
        <property type="protein sequence ID" value="CAA04955.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09795.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517467.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="O33522"/>
    <dbReference type="SMR" id="O33522"/>
    <dbReference type="STRING" id="266264.Rmet_2922"/>
    <dbReference type="PRIDE" id="O33522"/>
    <dbReference type="EnsemblBacteria" id="ABF09795">
        <property type="protein sequence ID" value="ABF09795"/>
        <property type="gene ID" value="Rmet_2922"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2922"/>
    <dbReference type="eggNOG" id="COG0443">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005965_2_1_4"/>
    <dbReference type="OMA" id="ISIKRHM"/>
    <dbReference type="OrthoDB" id="161217at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.34.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00332">
        <property type="entry name" value="DnaK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012725">
        <property type="entry name" value="Chaperone_DnaK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018181">
        <property type="entry name" value="Heat_shock_70_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029048">
        <property type="entry name" value="HSP70_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029047">
        <property type="entry name" value="HSP70_peptide-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013126">
        <property type="entry name" value="Hsp_70_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19375">
        <property type="entry name" value="PTHR19375"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00012">
        <property type="entry name" value="HSP70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100920">
        <property type="entry name" value="SSF100920"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100934">
        <property type="entry name" value="SSF100934"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="SSF53067"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02350">
        <property type="entry name" value="prok_dnaK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00297">
        <property type="entry name" value="HSP70_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00329">
        <property type="entry name" value="HSP70_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01036">
        <property type="entry name" value="HSP70_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0143">Chaperone</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0597">Phosphoprotein</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0346">Stress response</keyword>
    <feature type="chain" id="PRO_0000078406" description="Chaperone protein DnaK">
        <location>
            <begin position="1"/>
            <end position="648"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="612"/>
            <end position="635"/>
        </location>
    </feature>
    <feature type="modified residue" description="Phosphothreonine; by autocatalysis" evidence="1">
        <location>
            <position position="200"/>
        </location>
    </feature>
    <feature type="sequence conflict" description="In Ref. 1; CAA04955." evidence="3" ref="1">
        <original>VL</original>
        <variation>AV</variation>
        <location>
            <begin position="119"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="sequence conflict" description="In Ref. 1; CAA04955." evidence="3" ref="1">
        <original>SQR</original>
        <variation>AQC</variation>
        <location>
            <begin position="149"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="sequence conflict" description="In Ref. 1; CAA04955." evidence="3" ref="1">
        <original>K</original>
        <variation>R</variation>
        <location>
            <position position="247"/>
        </location>
    </feature>
    <feature type="sequence conflict" description="In Ref. 1; CAA04955." evidence="3" ref="1">
        <original>L</original>
        <variation>C</variation>
        <location>
            <position position="263"/>
        </location>
    </feature>
    <feature type="sequence conflict" description="In Ref. 1; CAA04955." evidence="3" ref="1">
        <original>EYGEKLEAGEKEKIEAAIKDLEDAARGGD</original>
        <variation>RVRREARSWREGKDRSRDQGPGRRRPRVN</variation>
        <location>
            <begin position="553"/>
            <end position="581"/>
        </location>
    </feature>
    <feature type="sequence conflict" description="In Ref. 1." evidence="3" ref="1">
        <original>GGAQQQAQPQDDNVVDAEFKEVNDKK</original>
        <variation>RFSSRPSRRRQRVGCRVQGSERQEVIGDG</variation>
        <location>
            <begin position="623"/>
            <end position="648"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="648" mass="69787" checksum="0E962FFF93C6CCA4" modified="2006-07-11" version="2"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="53" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LAE7</accession>
    <name>Q1LAE7_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_6020</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2 3">
        <name>pMOL30</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid pMOL30</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000354">
        <property type="protein sequence ID" value="ABF12879.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF12879">
        <property type="protein sequence ID" value="ABF12879"/>
        <property type="gene ID" value="Rmet_6020"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6020"/>
    <dbReference type="HOGENOM" id="CLU_2536089_0_0_4"/>
    <dbReference type="OMA" id="APLVHTF"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid pMOL30"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="60"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF12879.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="86" mass="9435" checksum="FC8676D18A916092" modified="2006-05-30" version="1">MMPTMTALVAARVALVICAFIFLIAFQRRKRLFTVKTGNASSVPPDFDAFVTDLLVENRLTLVLSGFFATLCLVYVVYTLFVPGAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="68" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LAU4</accession>
    <name>Q1LAU4_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Peptidase (C39-family, bacteriocin processing)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_5873</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12732.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518082.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5873"/>
    <dbReference type="EnsemblBacteria" id="ABF12732">
        <property type="protein sequence ID" value="ABF12732"/>
        <property type="gene ID" value="Rmet_5873"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5873"/>
    <dbReference type="eggNOG" id="COG3271">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_092029_0_0_4"/>
    <dbReference type="OMA" id="YWLDVDE"/>
    <dbReference type="OrthoDB" id="1287580at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008233">
        <property type="term" value="F:peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005074">
        <property type="entry name" value="Peptidase_C39"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03412">
        <property type="entry name" value="Peptidase_C39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50990">
        <property type="entry name" value="PEPTIDASE_C39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193253" evidence="1">
        <location>
            <begin position="26"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase C39" evidence="2">
        <location>
            <begin position="56"/>
            <end position="186"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF12732.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="232" mass="25959" checksum="2911D8C2498885C4" modified="2006-05-30" version="1">MIMRSLLLAAATLAVALGGIAEARADTIRIPGDQGNSYTVPVTSLREARFRTTIRQQYDFSCGSAAVATLLTFQYGFPVNEETVFANMYVNGDQAKIRSEGFSLLDMKRFLESRGFLADGYELPLSKLEEAQIPAIVLIVENGYHHFVVIKGIKGDRVLIGDPARGTRSIERDHFEKIWDSQLLFVIHNRTDRARFNLAADWRVAPSGPYWMGVPRDSLFFTVMPKHGPADF</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LAU6</accession>
    <name>Q1LAU6_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_5871</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12730.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518080.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5871"/>
    <dbReference type="EnsemblBacteria" id="ABF12730">
        <property type="protein sequence ID" value="ABF12730"/>
        <property type="gene ID" value="Rmet_5871"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5871"/>
    <dbReference type="eggNOG" id="ENOG502ZC0P">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_040800_0_0_4"/>
    <dbReference type="OMA" id="MGFADKI"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004192974" evidence="2">
        <location>
            <begin position="23"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="19"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="383"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="20"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="386"/>
            <end position="405"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF12730.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="493" mass="42823" checksum="D1EE981209EC8690" modified="2006-05-30" version="1">MNKTLLASAIALAFGIGGQALANPTNNNEKTGTTNTQNATATSTQSGAGASANENSTALQANGNSSSSTTNKTKNTTNTNTNSKNHTDQDMHGYGNAAAANGSTATANFTNSFNSSKALAISKLEGYVTGNYIHNIGNVATNNGTANGGAGGKGGAGYGGDGKGGSGGSGGASGAGGGGGSGGSAINGSAKAGYASNGSATAGDGAAGGKAVGWAGDGGNAKSNGGGALTASLGVGGFGGSGKGSGGAAGAGALGGFGGDGGNAKGSGGGALGASGGLGGDSAAGSALGKVHGNAGNSSATSGAGGNGTGGTASGTGGAGSGTGGAGGAGGAATAAAAGGAGTGSGGAGGANTSTAGAATGGAATSTGGAGGAQNSVAGNGGAGGAATNGSASSMASNGSATAGNGAVGASSGAGGAGGVGMGGTGGTGNGAVGGAGGNGGTNYVDAGTFNMSNMMSSVGQSAAGIMMANQNSGMSSLVQQSVNVQANLAVGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="55" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LBC0</accession>
    <name>Q1LBC0_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_5697</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12556.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011520097.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF12556">
        <property type="protein sequence ID" value="ABF12556"/>
        <property type="gene ID" value="Rmet_5697"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5697"/>
    <dbReference type="HOGENOM" id="CLU_2275142_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025421">
        <property type="entry name" value="DUF4148"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13663">
        <property type="entry name" value="DUF4148"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193275" evidence="2">
        <location>
            <begin position="29"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="80"/>
            <end position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF12556.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="102" mass="10810" checksum="8B11DD998048626E" modified="2006-05-30" version="1">MESGSFATMCRACAIVLPMIAVCGYARAQTTTGTAPPPVTRASILRELQALKSVGFDINDENYPNSLQQSLRKLEAMEATHQTEAGAAVQGSEKPAMPSVPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-10-07" version="49" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LBC3</accession>
    <name>Q1LBC3_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_5694</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12553.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5694"/>
    <dbReference type="DNASU" id="4042558"/>
    <dbReference type="EnsemblBacteria" id="ABF12553">
        <property type="protein sequence ID" value="ABF12553"/>
        <property type="gene ID" value="Rmet_5694"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5694"/>
    <dbReference type="eggNOG" id="ENOG50330EQ">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1293431_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="101"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="132"/>
            <end position="155"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF12553.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="213" mass="21759" checksum="8EFF536995DC0E54" modified="2006-05-30" version="1">MNCDDSNCLHRLGGIGAEALRAGSICSAAPAVNWRLMAGQGVAVVAAIATADFIRPRNAPPLTSAHDPVTQCNGSSSMMESYKRGQSLAVKESEMRKSIRYTGIAVLSAAMLVAGSASAHDRYYRGGYHGGGGSSAGAALAVAAVAGLAIGALVASSQPVMAAPAPVYAPPQPVAYAAPQVPPGYCYRSYDGAYVPCRGAAPVNYAPQPYYGY</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LBE8</accession>
    <name>Q1LBE8_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Copper resistance C protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">copC2</name>
        <name evidence="6" type="ordered locus">Rmet_5669</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="7">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="7">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CopC family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12528.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011520074.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5669"/>
    <dbReference type="EnsemblBacteria" id="ABF12528">
        <property type="protein sequence ID" value="ABF12528"/>
        <property type="gene ID" value="Rmet_5669"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5669"/>
    <dbReference type="eggNOG" id="COG2372">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_087859_4_2_4"/>
    <dbReference type="OMA" id="GHKTKGS"/>
    <dbReference type="OrthoDB" id="1753926at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042597">
        <property type="term" value="C:periplasmic space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046688">
        <property type="term" value="P:response to copper ion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1220">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007348">
        <property type="entry name" value="CopC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014755">
        <property type="entry name" value="Cu-Rt/internalin_Ig-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04234">
        <property type="entry name" value="CopC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="SSF81296"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0186">Copper</keyword>
    <keyword evidence="6" id="KW-0614">Plasmid</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193293" evidence="4">
        <location>
            <begin position="26"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="domain" description="CopC" evidence="5">
        <location>
            <begin position="26"/>
            <end position="126"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010509"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF04234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABF12528.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="128" mass="13219" checksum="5A90D21095D1B50E" modified="2006-05-30" version="1">MTYIHTAAKALVAATALLASAAALAHPKLVSASPADKSEGPAPTRIELLFSENLVKQVSGANLVMTGMPGMADHPPMKIAASVSGGDDPKAMIITPKSPLVPGTYRLDWRAVSSDTHPVNGSITFTTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="66" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LBM2</accession>
    <name>Q1LBM2_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Membrane protein related to Kef-type K+ transport system, predicted NAD-binding component</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_5595</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12454.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011520003.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5595"/>
    <dbReference type="EnsemblBacteria" id="ABF12454">
        <property type="protein sequence ID" value="ABF12454"/>
        <property type="gene ID" value="Rmet_5595"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5595"/>
    <dbReference type="eggNOG" id="ENOG5031669">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_116321_1_0_4"/>
    <dbReference type="OMA" id="DYFYFSL"/>
    <dbReference type="OrthoDB" id="1991946at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013099">
        <property type="entry name" value="K_chnl_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07885">
        <property type="entry name" value="Ion_trans_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="47"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="119"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="domain" description="Ion_trans_2" evidence="2">
        <location>
            <begin position="87"/>
            <end position="140"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF12454.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="154" mass="16925" checksum="59698734C8117ABA" modified="2006-05-30" version="1">MPDTPINYLLAVGLTAGLTVICVGLHYEALRLISSIHPKRLSGKLSIAVMILLIILTHCVEAVVFSGGYWLGTELLGIGRLSGMQEHGPAAYIYFSLETFTTQSIGDIVPIGQLRLLAAVQPLVGLILIGWSTSFTFLIMRRDWQWDDERGARN</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-10-07" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LBQ0</accession>
    <name>Q1LBQ0_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_5567</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12426.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5567"/>
    <dbReference type="EnsemblBacteria" id="ABF12426">
        <property type="protein sequence ID" value="ABF12426"/>
        <property type="gene ID" value="Rmet_5567"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5567"/>
    <dbReference type="eggNOG" id="ENOG5032PTC">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1371235_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF12426.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="199" mass="22723" checksum="65F6C4DCDFB5A1C6" modified="2006-05-30" version="1">MRSLYSSSCMRLQRVAANPYTLYLLGLTTVSYAHPIYSVFSNLNRVAYEDGIDNEYIRWDREKRKFIGNSIYISQIDSLTTEFPRGAEFMYDRCIHYSLAVGCEGRDWHDVLAQMVGFHPAGGAEPGEEVPFREILHPSRTSATYGPKAAQKLVEDFAAWEERAKTFADPEFYLAYWWLSCCFGHASKGGAVQMNWVEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LBS9</accession>
    <name>Q1LBS9_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Putative acyl-CoA dehydrogenase</fullName>
            <ecNumber evidence="8">1.3.99.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Rmet_5538</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="9">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="9">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the acyl-CoA dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.3.99.-" evidence="8"/>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12397.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519943.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5538"/>
    <dbReference type="EnsemblBacteria" id="ABF12397">
        <property type="protein sequence ID" value="ABF12397"/>
        <property type="gene ID" value="Rmet_5538"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5538"/>
    <dbReference type="eggNOG" id="COG1960">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018204_3_5_4"/>
    <dbReference type="OMA" id="HNSFAHA"/>
    <dbReference type="OrthoDB" id="760677at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016627">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-CH group of donors"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.540.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.110.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006091">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046373">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036250">
        <property type="entry name" value="AcylCo_DH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009075">
        <property type="entry name" value="AcylCo_DH/oxidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013786">
        <property type="entry name" value="AcylCoA_DH/ox_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037069">
        <property type="entry name" value="AcylCoA_DH/ox_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009100">
        <property type="entry name" value="AcylCoA_DH/oxidase_NM_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00441">
        <property type="entry name" value="Acyl-CoA_dh_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02770">
        <property type="entry name" value="Acyl-CoA_dh_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02771">
        <property type="entry name" value="Acyl-CoA_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47203">
        <property type="entry name" value="SSF47203"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56645">
        <property type="entry name" value="SSF56645"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0274">FAD</keyword>
    <keyword evidence="3" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-0614">Plasmid</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Acyl-CoA_dh_N" evidence="7">
        <location>
            <begin position="10"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA_dh_M" evidence="6">
        <location>
            <begin position="127"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA_dh_1" evidence="5">
        <location>
            <begin position="237"/>
            <end position="382"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF02770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF02771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ABF12397.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="384" mass="42174" checksum="C49654E75D35AE55" modified="2006-05-30" version="1">MSLINTVGLTEDQRMMREGVLDLLNRVLPWEKVRKMDEAREFPLEAYEALADAGYIGLFYPEHLGGMGGSYRDITVLLETLGYYYTGIAQAITTSAVYAGMHIAKFGTPEMQQELIPKIISGKAKLALCMSEPGTGSDVAGLKTYAEPAGDEYILNGNKVWITCGHVADYFVVITRTDREGGRHQGISTLLVDAKAPGVTVRPLQMLGRRTTHANEVFFDNVRVPAKNLFGGEGRAWKNIMSCLGLERLGLAAISAGHCFKITEYARDYAKERVQFGKPISDFQVIQHKLADMLILSETARQVVYRAADVLDAGQAAVTEASIAKVVASENNFKCADIGMQIMAGAGYSMEYDMQMFFRDSRVGPIGGGTNEIQRNILAKQMDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LBT8</accession>
    <name>Q1LBT8_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Putative acyl-CoA dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Rmet_5529</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="9">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="9">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the acyl-CoA dehydrogenase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12388.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519932.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5529"/>
    <dbReference type="EnsemblBacteria" id="ABF12388">
        <property type="protein sequence ID" value="ABF12388"/>
        <property type="gene ID" value="Rmet_5529"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5529"/>
    <dbReference type="eggNOG" id="COG1960">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018204_5_2_4"/>
    <dbReference type="OMA" id="WESGIHV"/>
    <dbReference type="OrthoDB" id="760677at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016627">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-CH group of donors"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.540.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.110.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006091">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046373">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036250">
        <property type="entry name" value="AcylCo_DH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009075">
        <property type="entry name" value="AcylCo_DH/oxidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013786">
        <property type="entry name" value="AcylCoA_DH/ox_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037069">
        <property type="entry name" value="AcylCoA_DH/ox_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009100">
        <property type="entry name" value="AcylCoA_DH/oxidase_NM_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00441">
        <property type="entry name" value="Acyl-CoA_dh_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02770">
        <property type="entry name" value="Acyl-CoA_dh_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02771">
        <property type="entry name" value="Acyl-CoA_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47203">
        <property type="entry name" value="SSF47203"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56645">
        <property type="entry name" value="SSF56645"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0274">FAD</keyword>
    <keyword evidence="3" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="8" id="KW-0614">Plasmid</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Acyl-CoA_dh_N" evidence="7">
        <location>
            <begin position="6"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA_dh_M" evidence="6">
        <location>
            <begin position="139"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA_dh_1" evidence="5">
        <location>
            <begin position="231"/>
            <end position="374"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF02770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF02771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ABF12388.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="379" mass="40859" checksum="F99C3DD2893DEBF3" modified="2006-05-30" version="1">MNFELNEDQRALQESVHRLLNERYGFENRRQYVEADAGHDEKTWSALSELGLTALLVPEDLEGFGGGAVDLFPVMQELGAVLSLEPLLATNFLPATALRTYAGDARAKEALQSIASGERRIAWAHDEVEARHTDWWISTRATKTGEKWLLSGQKFNVVGANDAHQYLVSARVQGSTEDTDGCALFLVNADAKGLSVRNYKLVDDTPAGELTFSNVSALPLGEAGDGHQSLRAIESALAMGIAAACADMVGAAGAAFQLAIEYVNTRKQFGRLIGENQALRHRASEMLVSLELAKSMAIAAAVAVDDQDSEGAELDLHRAKLSVAKHARAVAHGAIQLHGGIGMTEEYAVGHYLRRVHVLDQLFGDGEAHTARLASLLHA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-10-07" version="51" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LBY1</accession>
    <name>Q1LBY1_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_5486</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12345.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5486"/>
    <dbReference type="EnsemblBacteria" id="ABF12345">
        <property type="protein sequence ID" value="ABF12345"/>
        <property type="gene ID" value="Rmet_5486"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5486"/>
    <dbReference type="eggNOG" id="ENOG5031DR3">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_923994_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="6"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="148"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="190"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="280"/>
            <end position="301"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF12345.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="301" mass="30976" checksum="F0C756B904A79F26" modified="2006-05-30" version="1">MVAAGVSALILALIVIAAVFALGVRTIFPDPSRSLRWPAEQSGLNGIVAWAHGGDTGGKQPNAEFNAARLNGEAVDRWITRCTSDLLQKPVINARVRAQFGKYGESYAADPSNLLTQDAVLPAATDLRFLSKSLLIGRGENALAAANAYLVLQIATLVAIALGLATTVLVSLSSTEFGKGDGRTARAIRILAIVFPALGTATAAVAAFYAPGQSYARASQALAGFRQVHDQIASDLGELTCPTSSDPGAANDLSAKLAGWKKSLRDVQSLAQAAALAAADATRGQVQPGGGVGNTGDGQKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="57" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LC02</accession>
    <name>Q1LC02_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_5465</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12324.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519869.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF12324">
        <property type="protein sequence ID" value="ABF12324"/>
        <property type="gene ID" value="Rmet_5465"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5465"/>
    <dbReference type="eggNOG" id="ENOG502ZX0F">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_140206_0_0_4"/>
    <dbReference type="OMA" id="DMFEMAS"/>
    <dbReference type="OrthoDB" id="2179521at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF12324.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="163" mass="18726" checksum="60222BE7B2566D04" modified="2006-05-30" version="1">MSLGPFYAAVKNLTKLEGYDPDYPGTWPHRTIEIEVDAEYPEYPEGLEVGALYQYESEENYWLNADQDMYFFKWREKLVQMVGEKSVEEAEGQFPFCELIRHQGSTGGTIGPVVCEKIASDFDAWEAAARSLGDERFFEYYVHIRRAFEFARGTGAVSLCVLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="59" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LC06</accession>
    <name>Q1LC06_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_5461</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12320.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF12320">
        <property type="protein sequence ID" value="ABF12320"/>
        <property type="gene ID" value="Rmet_5461"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5461"/>
    <dbReference type="eggNOG" id="ENOG5034BYR">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_122731_0_0_4"/>
    <dbReference type="OMA" id="MENCWAK"/>
    <dbReference type="OrthoDB" id="2072161at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045526">
        <property type="entry name" value="DUF6471"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20075">
        <property type="entry name" value="DUF6471"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF6471" evidence="1">
        <location>
            <begin position="2"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="domain" description="DUF6471" evidence="1">
        <location>
            <begin position="75"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF20075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF12320.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="164" mass="18320" checksum="BEE447D9C0F062ED" modified="2006-05-30" version="1">MATRTARGVLARKDFSFAQLAERISVDGMPESLRGTESKIHRGTYSLAFFLAMLDVVGAEYPTQWTKFIDSNKSWENAAKHVFLHEMKAHGLSVKDLSDRLEELGISLKPEKVEAQLANGELPFTAFLQLALVVPVSEFTRFVDRNDLLEAAGDREFSAFQGKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LC86</accession>
    <name>Q1LC86_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">AMP-dependent synthetase and ligase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_5381</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12240.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519787.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5381"/>
    <dbReference type="EnsemblBacteria" id="ABF12240">
        <property type="protein sequence ID" value="ABF12240"/>
        <property type="gene ID" value="Rmet_5381"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5381"/>
    <dbReference type="eggNOG" id="COG0318">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000022_59_0_4"/>
    <dbReference type="OMA" id="GQTECYP"/>
    <dbReference type="OrthoDB" id="377638at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016874">
        <property type="term" value="F:ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025110">
        <property type="entry name" value="AMP-bd_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045851">
        <property type="entry name" value="AMP-bd_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020845">
        <property type="entry name" value="AMP-binding_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_Synth/Lig"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13193">
        <property type="entry name" value="AMP-binding_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00455">
        <property type="entry name" value="AMP_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0436">Ligase</keyword>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AMP-binding" evidence="1">
        <location>
            <begin position="7"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="domain" description="AMP-binding_C" evidence="2">
        <location>
            <begin position="415"/>
            <end position="490"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF12240.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="510" mass="55105" checksum="33653E28FC0459FB" modified="2006-05-30" version="1">MLPIELFWRAADRWPDNVAIDAPGGSLRYRELAGQVAALATHFMTLDPAPQSRVGICAGNSAEHIVALLAVLASGKIWVPLNPKSTQPELRRIIDATTPSIVVLDAASEALLGDAPGTRLYTDYADHASGGADVLSAVVLRYPDATRPVLDLHRDATQAIKFTGGTTGAPKGVMQPYRAWMTNIVNQIHAWGFTEADRYVMAAPITHGTSTYVMPILAQGGCHVIMEGSGAEAVRATFRDRAGTVCFMPPTLIYMLMALPGTSRADYPALRRLIYGGAPMPPQKIQQAREFFGSVVATTYGQTEAPQLLTVIGPDDLADERRMASVGRATWFSEVAIMSPDGRLLPRGEVGEVVARGDLVMTGYWNRPDLTAATIVDGWLHTGDRGMLDAQGFLYLKDRLREVVITGGFNVYPIDVENALGQHPAVHECAVFGVPDDKWGEAVHAAVQLREGCAVQATELIAFVRERLGPVQTPKQIHFHTSLPRSTVGKVLKNTVRDQALTALQTGDQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="98" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LCA7</accession>
    <name>Q1LCA7_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Putative transcriptional regulator, LysR family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Rmet_5360</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="5">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="5">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the LysR transcriptional regulatory family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12219.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519765.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5360"/>
    <dbReference type="EnsemblBacteria" id="ABF12219">
        <property type="protein sequence ID" value="ABF12219"/>
        <property type="gene ID" value="Rmet_5360"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5360"/>
    <dbReference type="eggNOG" id="COG0583">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039613_39_0_4"/>
    <dbReference type="OMA" id="MEDEGMA"/>
    <dbReference type="OrthoDB" id="1591366at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd08417">
        <property type="entry name" value="PBP2_Nitroaromatics_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005119">
        <property type="entry name" value="LysR_subst-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000847">
        <property type="entry name" value="Tscrpt_reg_HTH_LysR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037402">
        <property type="entry name" value="YidZ_PBP2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00126">
        <property type="entry name" value="HTH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03466">
        <property type="entry name" value="LysR_substrate"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00039">
        <property type="entry name" value="HTHLYSR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50931">
        <property type="entry name" value="HTH_LYSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0614">Plasmid</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH lysR-type" evidence="3">
        <location>
            <begin position="35"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF12219.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="331" mass="36641" checksum="43BD26E6EB4F1846" modified="2006-05-30" version="1">MNESRFSDTPGSDGTETPEGRDSRRPALAELRKKDLNLLVVLAAMLESNSVSEAARRLDSTQPSMSRMLDRLRDDLGDPLLIKSAHKMLPTRRAQDIRRVLDDVLRAIEGVYQQGSDYNPAMETGTYVIGINDSLQALIAPAFLASLRQVAPRARVRMQPVPQQGGISAMTSGAIDLMVAFYPVDMESLRSELLLTTRFGCLSARSNPHIGAITSARELASLDCLDISQFGLITRLLDRYFQSHGLHRRIVGTLTSYLAVADAIHDTEIYAMIPDYLARPLCRHPGMRFSPMEDEGMAVPIHLCWHNNVHSHPFVTVLRSLLASAARHVAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LCB7</accession>
    <name>Q1LCB7_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_5350</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12209.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5350"/>
    <dbReference type="EnsemblBacteria" id="ABF12209">
        <property type="protein sequence ID" value="ABF12209"/>
        <property type="gene ID" value="Rmet_5350"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5350"/>
    <dbReference type="eggNOG" id="COG0446">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030742_2_0_4"/>
    <dbReference type="OMA" id="FPWVYYN"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015904">
        <property type="entry name" value="Sulphide_quinone_reductase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10632">
        <property type="entry name" value="PTHR10632"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="SSF51905"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF12209.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="396" mass="43400" checksum="0420C7CFCAB32F79" modified="2006-05-30" version="1">MASSLLARKPGLDIAIIDPADIHYYQPGWTLVGGGVFEPAHTARTMASVLPRGVSWIKATVTGFEPEANAVMLEGNRVVKYEHLVVCPGLKLDWDAIEGLPQALGRNGVTSNYRYDLAPYTWKLVQELVRKQGGKALFSQPPMPIKCAGAPQKAMYLSADAWRRAGVLDRVDIQFCNAGNVLFGVADYVPALMEYVERYAIDLNFGETLVAVDGPARCATFRRANADGTTALVTREFDMLHAVPPQKAPDFIRSSPLADAAGWIDVDPATLRHTRFGNIFGLGDAGNTTNAKTAAAARKQAPVVAHNVLAERGSARGEARYDGYGSCPLTVERGKIVLAEFTYGGKVAPTFPSWFIEGKRPSRMAWWLKERILPPLYWRGMLKGREWLANPELQGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="101" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LCT2</accession>
    <name>Q1LCT2_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 6">Carbonic anhydrase</fullName>
            <ecNumber evidence="3 6">4.2.1.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Carbonate dehydratase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Rmet_5185</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="8">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="8">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Reversible hydration of carbon dioxide.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 6">
            <text>H(+) + hydrogencarbonate = CO2 + H2O</text>
            <dbReference type="Rhea" id="RHEA:10748"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <dbReference type="EC" id="4.2.1.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the beta-class carbonic anhydrase family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.1" evidence="3 6"/>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12044.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519591.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5185"/>
    <dbReference type="EnsemblBacteria" id="ABF12044">
        <property type="protein sequence ID" value="ABF12044"/>
        <property type="gene ID" value="Rmet_5185"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5185"/>
    <dbReference type="eggNOG" id="COG0288">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_053879_5_3_4"/>
    <dbReference type="OMA" id="CHNCEKE"/>
    <dbReference type="OrthoDB" id="1841447at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004089">
        <property type="term" value="F:carbonate dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015976">
        <property type="term" value="P:carbon utilization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00884">
        <property type="entry name" value="beta_CA_cladeB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1050.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045066">
        <property type="entry name" value="Beta_CA_cladeB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001765">
        <property type="entry name" value="Carbonic_anhydrase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015892">
        <property type="entry name" value="Carbonic_anhydrase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036874">
        <property type="entry name" value="Carbonic_anhydrase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11002">
        <property type="entry name" value="PTHR11002"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00484">
        <property type="entry name" value="Pro_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00947">
        <property type="entry name" value="Pro_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53056">
        <property type="entry name" value="SSF53056"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00704">
        <property type="entry name" value="PROK_CO2_ANHYDRASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00705">
        <property type="entry name" value="PROK_CO2_ANHYDRASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6 7" id="KW-0456">Lyase</keyword>
    <keyword evidence="7" id="KW-0614">Plasmid</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0862">Zinc</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012925"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003956"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABF12044.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="225" mass="24975" checksum="C2E168C02EE27377" modified="2006-05-30" version="1">MRKLIMGIVEFREKMLPRYAEQFSKLALAQTPDALFITCSDSRVVPDLLASTHPGDLFTMRNVGNLIPPATAEGVSTGDLSEASAIEYAVLVLKVANIVVCGHSECGAMKAVYSRNPKLKAPNLDKWLYHASNAAFRLEHEGALDESLKPHDQLSQLNVLVQIEHLMTYPIVRRQVMAGALVLSGWWFDIATGDMYAYERASRSFEVIDRALAERLASRLASRAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LD29</accession>
    <name>Q1LD29_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative oligopeptide/dipeptide ABC transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">dppA</name>
        <name evidence="3" type="ordered locus">Rmet_5088</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11947.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519494.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5088"/>
    <dbReference type="EnsemblBacteria" id="ABF11947">
        <property type="protein sequence ID" value="ABF11947"/>
        <property type="gene ID" value="Rmet_5088"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5088"/>
    <dbReference type="eggNOG" id="COG0747">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017028_7_1_4"/>
    <dbReference type="OMA" id="GKPQPQM"/>
    <dbReference type="OrthoDB" id="162819at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043190">
        <property type="term" value="C:ATP-binding cassette (ABC) transporter complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030288">
        <property type="term" value="C:outer membrane-bounded periplasmic space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030678">
        <property type="entry name" value="Peptide/Ni-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039424">
        <property type="entry name" value="SBP_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000914">
        <property type="entry name" value="SBP_5_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30290">
        <property type="entry name" value="PTHR30290"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00496">
        <property type="entry name" value="SBP_bac_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002741">
        <property type="entry name" value="MppA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193354" evidence="1">
        <location>
            <begin position="26"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="domain" description="SBP_bac_5" evidence="2">
        <location>
            <begin position="72"/>
            <end position="432"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF11947.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="527" mass="57479" checksum="B842D2BEB4C87A14" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-08-12" version="68" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LD80</accession>
    <name>Q1LD80_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Periplasmic copper-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">cusF</name>
        <name evidence="2" type="ordered locus">Rmet_5034</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11896.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5034"/>
    <dbReference type="EnsemblBacteria" id="ABF11896">
        <property type="protein sequence ID" value="ABF11896"/>
        <property type="gene ID" value="Rmet_5034"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5034"/>
    <dbReference type="eggNOG" id="COG5569">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_140852_0_1_4"/>
    <dbReference type="OMA" id="FAGNDMA"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.320">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021647">
        <property type="entry name" value="CusF_Ec"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042230">
        <property type="entry name" value="CusF_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11604">
        <property type="entry name" value="CusF_Ec"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193331" evidence="1">
        <location>
            <begin position="32"/>
            <end position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF11896.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="123" mass="12854" checksum="38981E6DD3441D96" modified="2006-05-30" version="1">MKTSLTSRASRASLHAAVFALAVGLMPPTFAAGMDGSAGMDMKPAAKAMKTPQPVSAEVRKVDPAAGKVTLKHGPIMNLGMPPMTMAFPVKDPAMLTNLKEGDKVSATFDNVDGKITVTDLRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-10-07" version="65" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LDJ4</accession>
    <name>Q1LDJ4_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative phospholipid/glycerol acyltransferase</fullName>
            <ecNumber evidence="2">2.3.1.51</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_4920</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EC" id="2.3.1.51" evidence="2"/>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11782.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4920"/>
    <dbReference type="EnsemblBacteria" id="ABF11782">
        <property type="protein sequence ID" value="ABF11782"/>
        <property type="gene ID" value="Rmet_4920"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4920"/>
    <dbReference type="eggNOG" id="COG0204">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_054727_0_0_4"/>
    <dbReference type="OMA" id="HHSWADI"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003841">
        <property type="term" value="F:1-acylglycerol-3-phosphate O-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002123">
        <property type="entry name" value="Plipid/glycerol_acylTrfase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01553">
        <property type="entry name" value="Acyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00563">
        <property type="entry name" value="PlsC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PlsC" evidence="1">
        <location>
            <begin position="88"/>
            <end position="230"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF11782.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="300" mass="34380" checksum="06EB3A998D5C4AA3" modified="2006-05-30" version="1">MKRLLAFLLGTLSLTLLALNTIFWCTPLFVLAVIKLLLPFAGVRRLIDPILNRIATSWISCNGVWIGLLQREPWAVEGLGSLRPDSWYLVNCNHRSWVDIFVLQRVLNRRIPLLKFFLKQQLIYVPVIGLAWWALDFPFMRRHSKAALRKNPELRNQDRETTRRACAKFARIPTSVMTFAEGTRYTPVKHRAQSSPYRHLLKPKAGSLAMALNAMGEQFHALLDVSIAYPDGTPTFWQLASGQAGRVMVHIRELPIPPDFCTSDYNSDATFRGEFHRWLGQVWEEKDEQIATMLAQAARP</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LDM4</accession>
    <name>Q1LDM4_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">M20_dimer domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Rmet_4890</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="6">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="6">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11752.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519311.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4890"/>
    <dbReference type="EnsemblBacteria" id="ABF11752">
        <property type="protein sequence ID" value="ABF11752"/>
        <property type="gene ID" value="Rmet_4890"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4890"/>
    <dbReference type="eggNOG" id="COG0624">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031786_2_1_4"/>
    <dbReference type="OMA" id="GYAGHIE"/>
    <dbReference type="OrthoDB" id="906744at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016805">
        <property type="term" value="F:dipeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036264">
        <property type="entry name" value="Bact_exopeptidase_dim_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010964">
        <property type="entry name" value="M20A_pepV-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002933">
        <property type="entry name" value="Peptidase_M20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011650">
        <property type="entry name" value="Peptidase_M20_dimer"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07687">
        <property type="entry name" value="M20_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01546">
        <property type="entry name" value="Peptidase_M20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55031">
        <property type="entry name" value="SSF55031"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01887">
        <property type="entry name" value="dipeptidaselike"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0224">Dipeptidase</keyword>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193036" description="M20_dimer domain-containing protein" evidence="3">
        <location>
            <begin position="30"/>
            <end position="579"/>
        </location>
    </feature>
    <feature type="domain" description="M20_dimer" evidence="4">
        <location>
            <begin position="361"/>
            <end position="470"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022997"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF07687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABF11752.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="579" mass="62209" checksum="88586EBD50266263" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LE59</accession>
    <name>Q1LE59_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative secretion type II protein related to protein G</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_4705</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11567.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519138.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4705"/>
    <dbReference type="EnsemblBacteria" id="ABF11567">
        <property type="protein sequence ID" value="ABF11567"/>
        <property type="gene ID" value="Rmet_4705"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4705"/>
    <dbReference type="eggNOG" id="COG2165">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_091705_7_2_4"/>
    <dbReference type="OMA" id="IDHYKDA"/>
    <dbReference type="OrthoDB" id="2044924at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015627">
        <property type="term" value="C:type II protein secretion system complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015628">
        <property type="term" value="P:protein secretion by the type II secretion system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000983">
        <property type="entry name" value="Bac_GSPG_pilin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012902">
        <property type="entry name" value="N_methyl_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045584">
        <property type="entry name" value="Pilin-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07963">
        <property type="entry name" value="N_methyl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00813">
        <property type="entry name" value="BCTERIALGSPG"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54523">
        <property type="entry name" value="SSF54523"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02532">
        <property type="entry name" value="IV_pilin_GFxxxE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00409">
        <property type="entry name" value="PROKAR_NTER_METHYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="24"/>
            <end position="46"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF11567.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="176" mass="18925" checksum="83731E1BD91B704F" modified="2006-05-30" version="1">MNARPGSLCIARRGPLTGCGGRGFTLIELVVTLALVGIVALAVLPFSELIVQRQKEQELRVALREIRTALDAYKEASDFGSIEKKADMSGYPPTLDVLATGVVDAKDPKGGLLQFLRRVPRDPFYAGDVDVPPESTWNVRAYGKAPDGSQAGSADVYDVSSTSTRVGLNGIPYKDW</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="62" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LE81</accession>
    <name>Q1LE81_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Thioredoxin-like_fold protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_4683</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11545.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519117.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4683"/>
    <dbReference type="EnsemblBacteria" id="ABF11545">
        <property type="protein sequence ID" value="ABF11545"/>
        <property type="gene ID" value="Rmet_4683"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4683"/>
    <dbReference type="eggNOG" id="COG3219">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_086594_0_1_4"/>
    <dbReference type="OMA" id="MARFHIR"/>
    <dbReference type="OrthoDB" id="1675974at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.690">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018640">
        <property type="entry name" value="DUF2063"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044922">
        <property type="entry name" value="DUF2063_N_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09836">
        <property type="entry name" value="DUF2063"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF2063" evidence="1">
        <location>
            <begin position="8"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF09836"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF11545.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="258" mass="27960" checksum="F95EF61A9EFDB246" modified="2006-05-30" version="1">MNANGHHFQDDFLRAILAPPGIAPPWTPITPLTQQPGFKVYRNTVLKGCVDALAANFPTVLRLVGEDWFRAAALIHVCAEPPRSVCLIEYGQEFADFLSGFEPAAALPYLPDVARLDRLWTESHLAADDEALDAAALATLNGGDLLGAALRPCASVRWRWFETPAFTIWRANREQRDLDGELEWIGEGALLTRPEGAVVWQHATQGMCAFLDACAAGANLQIASQSAMVAEPGIDIARLLATLLGARVFADIHGADIH</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="99" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LEM3</accession>
    <name>Q1LEM3_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Transcriptional activator for superoxide response Fe-S center for redox-sensing (MerR family)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">soxR</name>
        <name evidence="9" type="ordered locus">Rmet_4538</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="10">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="10">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11403.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518994.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4538"/>
    <dbReference type="EnsemblBacteria" id="ABF11403">
        <property type="protein sequence ID" value="ABF11403"/>
        <property type="gene ID" value="Rmet_4538"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4538"/>
    <dbReference type="eggNOG" id="COG0789">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_060077_5_1_4"/>
    <dbReference type="OMA" id="IHFYEAK"/>
    <dbReference type="OrthoDB" id="1677723at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006979">
        <property type="term" value="P:response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01110">
        <property type="entry name" value="HTH_SoxR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009061">
        <property type="entry name" value="DNA-bd_dom_put_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000551">
        <property type="entry name" value="MerR-type_HTH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010211">
        <property type="entry name" value="Redox-sen_tscrpt-act_SoxR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015358">
        <property type="entry name" value="Tscrpt_reg_MerR_DNA-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30204:SF0">
        <property type="entry name" value="PTHR30204:SF0"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00376">
        <property type="entry name" value="MerR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09278">
        <property type="entry name" value="MerR-DNA-bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00040">
        <property type="entry name" value="HTHMERR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00422">
        <property type="entry name" value="HTH_MERR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46955">
        <property type="entry name" value="SSF46955"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01950">
        <property type="entry name" value="SoxR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00552">
        <property type="entry name" value="HTH_MERR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50937">
        <property type="entry name" value="HTH_MERR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0001">2Fe-2S</keyword>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0408">Iron</keyword>
    <keyword evidence="3" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0614">Plasmid</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH merR-type" evidence="8">
        <location>
            <begin position="18"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="148"/>
            <end position="169"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ABF11403.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="169" mass="18521" checksum="999362F31AC1C22E" modified="2006-05-30" version="1">MKRALEDQEDHPLRSGDPLTVGEVAARSGVAVSALHFYESKGLICSTRSAGNQRRYPRGILRRLAVIRVAQRMGLPLSVIAEAFSRLPDDKAPTVADWRRLSAVWRADLDARIRTLTQLRDQLDGCIGCGCLSLKECPLRNPQDALAEEGPGPHFGAGPCEHRKSGKAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LF70</accession>
    <name>Q1LF70_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Extra-cytoplasmic solute receptor, uncharacterized protein UPF0065</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">bug</name>
        <name evidence="4" type="ordered locus">Rmet_4341</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="5">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="5">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Periplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the UPF0065 (bug) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11206.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518828.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4341"/>
    <dbReference type="EnsemblBacteria" id="ABF11206">
        <property type="protein sequence ID" value="ABF11206"/>
        <property type="gene ID" value="Rmet_4341"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4341"/>
    <dbReference type="eggNOG" id="COG3181">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045683_0_0_4"/>
    <dbReference type="OMA" id="VVESWYG"/>
    <dbReference type="OrthoDB" id="447734at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042597">
        <property type="term" value="C:periplasmic space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005064">
        <property type="entry name" value="BUG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042100">
        <property type="entry name" value="Bug_dom1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42928">
        <property type="entry name" value="PTHR42928"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03401">
        <property type="entry name" value="TctC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017082">
        <property type="entry name" value="YflP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-0675">Receptor</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193136" evidence="3">
        <location>
            <begin position="23"/>
            <end position="320"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF11206.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="320" mass="33640" checksum="889CDF5D3C212A96" modified="2006-05-30" version="1">MLKSKSFALFMSLAALSLGVRAETYPSKPIMLVVPFAAGGTVNMMGRLVAEHMGEQLGQPVVVDNKAGAGGAIGAGFVAKATPDGYTLLLGSMGQAVQPLLTKRLPYDPKKLVPVALFATVSNVLAVSSNAPAKNVSDLVGYSKANPGKLNMASAGIGSINQLIGELFMSRTGANFVHVPYKGAGPAGVDLLSGQVQLIFANLPNVLPYARAGKVRLLAVASEKRDPAIPDVPTFAEAGVKDAVVESWYGLMAPAGTKPEVIKKLQDTLLVVTKDKRFISQLAEQGARPYPGSSADMTRLMEKEGKRWGEVIEHAKISLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LF94</accession>
    <name>Q1LF94_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Alkylhydroperoxidase AhpD core</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_4317</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11182.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518806.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4317"/>
    <dbReference type="EnsemblBacteria" id="ABF11182">
        <property type="protein sequence ID" value="ABF11182"/>
        <property type="gene ID" value="Rmet_4317"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4317"/>
    <dbReference type="eggNOG" id="COG2128">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_082760_6_0_4"/>
    <dbReference type="OMA" id="NRFGVTC"/>
    <dbReference type="OrthoDB" id="1893149at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004601">
        <property type="term" value="F:peroxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051920">
        <property type="term" value="F:peroxiredoxin activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1290.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029032">
        <property type="entry name" value="AhpD-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004675">
        <property type="entry name" value="AhpD_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003779">
        <property type="entry name" value="CMD-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02627">
        <property type="entry name" value="CMD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69118">
        <property type="entry name" value="SSF69118"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00778">
        <property type="entry name" value="ahpD_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="2" id="KW-0575">Peroxidase</keyword>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CMD" evidence="1">
        <location>
            <begin position="15"/>
            <end position="94"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02627"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF11182.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="145" mass="16473" checksum="9CD67B1C67C2E529" modified="2006-05-30" version="1">MTQRLDFYNANPNAIKALLGLEKRISESSLEKSLVELIRLRASLINGCAYCIDLHTADARKGGEDDRRLATLSVWRETPFFTDRERAALAWTEAVTLVSQNHVPDEVWTAVRPHFDDAELVDLTLLISTINTWNRFAISFRKTPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LG44</accession>
    <name>Q1LG44_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Protocatechuate (Dihydroxy benzoate) 3,4-dioxygenase, alpha subunit</fullName>
            <ecNumber evidence="5">1.13.11.3</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">pcaG</name>
        <name evidence="5" type="ordered locus">Rmet_4014</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="6">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="6">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the intradiol ring-cleavage dioxygenase family.</text>
    </comment>
    <dbReference type="EC" id="1.13.11.3" evidence="5"/>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10882.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518522.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4014"/>
    <dbReference type="EnsemblBacteria" id="ABF10882">
        <property type="protein sequence ID" value="ABF10882"/>
        <property type="gene ID" value="Rmet_4014"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4014"/>
    <dbReference type="eggNOG" id="COG3485">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027719_7_1_4"/>
    <dbReference type="OMA" id="YRFDIHL"/>
    <dbReference type="OrthoDB" id="1456634at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008199">
        <property type="term" value="F:ferric iron binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018578">
        <property type="term" value="F:protocatechuate 3,4-dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019439">
        <property type="term" value="P:aromatic compound catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03463">
        <property type="entry name" value="3_4-PCD_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.130.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000627">
        <property type="entry name" value="Intradiol_dOase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015889">
        <property type="entry name" value="Intradiol_dOase_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012786">
        <property type="entry name" value="Protocat_dOase_a"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00775">
        <property type="entry name" value="Dioxygenase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49482">
        <property type="entry name" value="SSF49482"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02423">
        <property type="entry name" value="protocat_alph"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00083">
        <property type="entry name" value="INTRADIOL_DIOXYGENAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 5" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="3 5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-0614">Plasmid</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="INTRADIOL_DIOXYGENAS" evidence="4">
        <location>
            <begin position="40"/>
            <end position="68"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS00083"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABF10882.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="189" mass="20309" checksum="192800AE7BEE8CED" modified="2006-05-30" version="1">MLYATASQTVGPYLHIGLAGLNCADLTADAPDLAAQRIVIEGRVIDGNGEPVPDGMVEIWQANPAGRYRHPEDKRELGLVPGFTGFGRVPTEGDGSFRFVTVKPGAVPDVEGRPQAPHIMVSVFMRGLVKHVASRIYFPEETTANAADGVLALVPAERRATLIAQPNGQNSYRWDVVLQGAGETVFFDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="102" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LG72</accession>
    <name>Q1LG72_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Two component transcriptional regulator, LuxR family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Rmet_3986</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="6">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="6">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10854.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518494.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3986"/>
    <dbReference type="EnsemblBacteria" id="ABF10854">
        <property type="protein sequence ID" value="ABF10854"/>
        <property type="gene ID" value="Rmet_3986"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3986"/>
    <dbReference type="eggNOG" id="COG2197">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_90_1_4"/>
    <dbReference type="OMA" id="SQKTVAM"/>
    <dbReference type="OrthoDB" id="1687028at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06170">
        <property type="entry name" value="LuxR_C_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016032">
        <property type="entry name" value="Sig_transdc_resp-reg_C-effctor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000792">
        <property type="entry name" value="Tscrpt_reg_LuxR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00196">
        <property type="entry name" value="GerE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00038">
        <property type="entry name" value="HTHLUXR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00421">
        <property type="entry name" value="HTH_LUXR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46894">
        <property type="entry name" value="SSF46894"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50043">
        <property type="entry name" value="HTH_LUXR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="5" id="KW-0614">Plasmid</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Response regulatory" evidence="4">
        <location>
            <begin position="7"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="domain" description="HTH luxR-type" evidence="3">
        <location>
            <begin position="150"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="2">
        <location>
            <position position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABF10854.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="217" mass="23577" checksum="65B4AB008698EF76" modified="2006-05-30" version="1">MIAKTIRVALADDHPLVVVALRDCLQRTPGFQVTCECTNGSELVDALTQNPVDVAVTDFSMGHGDTSLDGFNLLRKLHRRHPDTRIVVVSAQTNPGVVSRAMKLGARAFVSKEDELDEIVRACIHVTVSNGHFYSPSIRAVLDSATAAATAAPATDLTLRELDVVRLYAQGFQLTEIANKLGRSVSTISSQKTVAMRKLSVQTNTDLIRYAYENGLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LG86</accession>
    <name>Q1LG86_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative chemotaxis signal transduction protein (CheW)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_3972</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10840.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518479.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3972"/>
    <dbReference type="EnsemblBacteria" id="ABF10840">
        <property type="protein sequence ID" value="ABF10840"/>
        <property type="gene ID" value="Rmet_3972"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3972"/>
    <dbReference type="eggNOG" id="COG0835">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_048995_7_0_4"/>
    <dbReference type="OMA" id="IAQAPAW"/>
    <dbReference type="OrthoDB" id="1711729at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006935">
        <property type="term" value="P:chemotaxis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039315">
        <property type="entry name" value="CheW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036061">
        <property type="entry name" value="CheW-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002545">
        <property type="entry name" value="CheW-lke_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22617">
        <property type="entry name" value="PTHR22617"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01584">
        <property type="entry name" value="CheW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00260">
        <property type="entry name" value="CheW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50341">
        <property type="entry name" value="SSF50341"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50851">
        <property type="entry name" value="CHEW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CheW-like" evidence="1">
        <location>
            <begin position="1"/>
            <end position="147"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50851"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF10840.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="157" mass="16876" checksum="425BEB8BBCD86C18" modified="2006-05-30" version="1">MALFLLFRIGADRYALDSAEVAEVLPLGPVKQIPGAPEWVCGLMTRHGQPVPVIDITALATGTPATARASTRTALVHYRRARDSGTQPRVLGLRLEHANETLRCDPASFIDGGIDPTHARYLGPVRHDPRGLIQWVHVGALLPDAVHAILFPASSPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="101" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LGA6</accession>
    <name>Q1LGA6_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Rubredoxin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">Rmet_3952</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="10">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="10">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="6 7">
            <name>Fe(3+)</name>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
        </cofactor>
        <text evidence="6 7">Binds 1 Fe(3+) ion per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the rubredoxin family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10820.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008644636.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3952"/>
    <dbReference type="EnsemblBacteria" id="ABF10820">
        <property type="protein sequence ID" value="ABF10820"/>
        <property type="gene ID" value="Rmet_3952"/>
    </dbReference>
    <dbReference type="GeneID" id="60823457"/>
    <dbReference type="KEGG" id="rme:Rmet_3952"/>
    <dbReference type="eggNOG" id="COG1773">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_128747_1_1_4"/>
    <dbReference type="OMA" id="MSAYRCP"/>
    <dbReference type="OrthoDB" id="2047418at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00730">
        <property type="entry name" value="rubredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024922">
        <property type="entry name" value="Rubredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024934">
        <property type="entry name" value="Rubredoxin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024935">
        <property type="entry name" value="Rubredoxin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018527">
        <property type="entry name" value="Rubredoxin_Fe_BS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00301">
        <property type="entry name" value="Rubredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000071">
        <property type="entry name" value="Rubredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00163">
        <property type="entry name" value="RUBREDOXIN"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00202">
        <property type="entry name" value="RUBREDOXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50903">
        <property type="entry name" value="RUBREDOXIN_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0249">Electron transport</keyword>
    <keyword evidence="5 6 7" id="KW-0408">Iron</keyword>
    <keyword evidence="3 6 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0614">Plasmid</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Rubredoxin-like" evidence="8">
        <location>
            <begin position="8"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="13"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="16"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="46"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="49"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005337"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR000071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR000071-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50903"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ABF10820.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="61" mass="6969" checksum="B5173BF2FB2DD9E1" modified="2006-05-30" version="1">MQQEAVAYKTWVCLICGWVYDEELGLPEEGIAAGTRWEDIPDDWRCPECDVGKGDFSMIEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="102" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LGS3</accession>
    <name>Q1LGS3_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Putative transcriptional regulator, TetR family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Rmet_3783</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="5">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="5">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10653.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3783"/>
    <dbReference type="EnsemblBacteria" id="ABF10653">
        <property type="protein sequence ID" value="ABF10653"/>
        <property type="gene ID" value="Rmet_3783"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3783"/>
    <dbReference type="eggNOG" id="COG1309">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_952715_0_0_4"/>
    <dbReference type="OMA" id="LYVYVAN"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045892">
        <property type="term" value="P:negative regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001647">
        <property type="entry name" value="HTH_TetR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004111">
        <property type="entry name" value="Repressor_TetR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036271">
        <property type="entry name" value="Tet_transcr_reg_TetR-rel_C_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02909">
        <property type="entry name" value="TetR_C_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48498">
        <property type="entry name" value="SSF48498"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50977">
        <property type="entry name" value="HTH_TETR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0614">Plasmid</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH tetR-type" evidence="3">
        <location>
            <begin position="64"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="H-T-H motif" evidence="1">
        <location>
            <begin position="87"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF10653.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="292" mass="32124" checksum="D548417378F52343" modified="2006-05-30" version="1">MACGAIGDRIGIPPISSRSDRPTPMPAARKSPAAKVAAQPSPNPVPRRRGRPPKTESPIGAEPMLSRTTILQHAIKLTKTLPLDQISMVQLAKDFGVAPGLIHYYLGGRDQLVSGVLNDYYRQRTLRIPPLTGDWRADVERIARLSFQVAVENPGVSNYVASHNRYRLFQDVQPGETDYGLEFFNRMTSAIMQGGFSAEQVALGYHLLAQYLVAASMAEASRQLPAYHQAFIQNKLDSVSAEQYPGARFVSRSFSQLSSDTAFEEGLRITLDGIAAWQRDVKPAKKRSARSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LGW0</accession>
    <name>Q1LGW0_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Flagellar hook-filament junction protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">flgL</name>
        <name evidence="3" type="ordered locus">Rmet_3744</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10616.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518262.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3744"/>
    <dbReference type="EnsemblBacteria" id="ABF10616">
        <property type="protein sequence ID" value="ABF10616"/>
        <property type="gene ID" value="Rmet_3744"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3744"/>
    <dbReference type="eggNOG" id="COG1344">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024437_5_0_4"/>
    <dbReference type="OMA" id="MRVTQGM"/>
    <dbReference type="OrthoDB" id="1788350at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009424">
        <property type="term" value="C:bacterial-type flagellum hook"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005198">
        <property type="term" value="F:structural molecule activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071973">
        <property type="term" value="P:bacterial-type flagellum-dependent cell motility"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013384">
        <property type="entry name" value="Flagell_FlgL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001492">
        <property type="entry name" value="Flagellin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001029">
        <property type="entry name" value="Flagellin_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42792">
        <property type="entry name" value="PTHR42792"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00669">
        <property type="entry name" value="Flagellin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02550">
        <property type="entry name" value="flagell_flgL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0966">Cell projection</keyword>
    <keyword evidence="3" id="KW-0969">Cilium</keyword>
    <keyword evidence="3" id="KW-0282">Flagellum</keyword>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Flagellin_N" evidence="2">
        <location>
            <begin position="4"/>
            <end position="140"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00669"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF10616.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="412" mass="43147" checksum="D65A0F321F29187C" modified="2006-05-30" version="1">MRVATSTLYQQGLASMNNQQSKLLYVQQQLGTGRRILTPSDDPVAATRALGVAQAQSVNGQYATTRKQANASLGLEENTLQSVTTTIQNIQSLLVNAGNGTMSDTDRASLATALQGQYDQLMGLANADDGNGNYLFAGLQANTVPFAKNAAGAAVYQGDNSHQLLQVDVARQMESGNTGAEVFMSVTTNAGYVLNGNAGNAGTATYGPISVSDPTNPLYGTPLKLTFQPNATTGAMEYQVSNATTGTAIGTAQPYSDGTPINVGGVSFTVKGTPAAGDSLTAVPAQQAGADIFANLQNVISALKKPIDTGANTDAANANLQNVLATGIRQMGNSLDNVLTVRSSVGSRMQELDALDTIGQNRDLNSKSALSDLQDLNYASAITEYYQRQTALQGAQQSFMQIQGMNLFQYLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LH99</accession>
    <name>Q1LH99_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Succinylglutamate desuccinylase/aspartoacylase-family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Rmet_3605</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10477.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518020.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3605"/>
    <dbReference type="EnsemblBacteria" id="ABF10477">
        <property type="protein sequence ID" value="ABF10477"/>
        <property type="gene ID" value="Rmet_3605"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3605"/>
    <dbReference type="eggNOG" id="COG3608">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_056327_0_0_4"/>
    <dbReference type="OMA" id="TSMDFRF"/>
    <dbReference type="OrthoDB" id="632656at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016788">
        <property type="term" value="F:hydrolase activity, acting on ester bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007036">
        <property type="entry name" value="Aste_AspA"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04952">
        <property type="entry name" value="AstE_AspA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABF10477.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="324" mass="35194" checksum="E32FB8E423D5D793" modified="2006-05-30" version="1">MTQTAPAFDAYPVEVEFPDIGPYAESNTGIAYLHTFDSGLPGPHVMINALTHGNEVCGAITVAGLLDHGLRPRRGRLTLSFANVDAYARFDAAKPDASRFVDQDFNRVWTSAVLDDVSRDSSELRRARAMRPVIDTVDLLLDLHSMHEKSRPLIVSGPLDKGIALSRAIGAPADIIVDEGHPEGRRMRDYADFGDPASPRNALLIECGQHWETSAVDVARDSTARFLLSAGIVEEADLPAGWLRPLPAAQRVIRVTEPVVATSMDFRFAGPYTGLETFADAGTVIGWNDGAEVLTPYPNCVLVMPSLRQLRPGVTVVRLGRLEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LHU6</accession>
    <name>Q1LHU6_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Radical SAM</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_3408</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10280.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517844.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3408"/>
    <dbReference type="EnsemblBacteria" id="ABF10280">
        <property type="protein sequence ID" value="ABF10280"/>
        <property type="gene ID" value="Rmet_3408"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3408"/>
    <dbReference type="eggNOG" id="COG1032">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021572_4_2_4"/>
    <dbReference type="OMA" id="YRQFYFR"/>
    <dbReference type="OrthoDB" id="973846at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.30.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaB/NifB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017834">
        <property type="entry name" value="Hopanoid_synth-assoc_rSAM_HpnJ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034466">
        <property type="entry name" value="Methyltransferase_Class_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023404">
        <property type="entry name" value="rSAM_horseshoe"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00404">
        <property type="entry name" value="hopanetetrol_cyclitol_ether_sy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01123">
        <property type="entry name" value="methyltransferase_(Class_B)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03471">
        <property type="entry name" value="HpnJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Radical SAM core" evidence="2">
        <location>
            <begin position="192"/>
            <end position="415"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF10280.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="473" mass="53713" checksum="FD8C64105926743E" modified="2006-05-30" version="1">MKTLFLQAPSYDGFDGGAGSRYQAKREIKSFWYPTWLAQPAALVPDSRVLDAPADGLGVEQTLNIAVDYDLVIIHTSTPSFPTDARFAEQLKERRPDIMIGMVGAKAAVDPGGTLSATEAIDFVCREEFDYTCQDVAAGKPLAGIAGLSYRLPDGTIEHNPARPMIENMDELPFVAPVYKRDLKIQNYFIGYLMHPYVSIYTGRGCRSRCTFCLWPQTVGGHRYRTRSAQSVIDEVKWIKENMPEVREIMFDDDTFTDFKPRVEEIARGLGEIGLPWSCNAKANVPYNTLKIMKENGLRLLLVGYESGDDQILLNIKKGLRTDIARRFTEDCRKLGIQIHGTFILGLPGETRDTIEKTIEYAKEINPHTIQVSLAAPYPGTTLYKQAVDNGWLEENKVIHLVNDSGVQLAAISYPHLSREEIYHGVETFYKRFYFRPSKIWEIVREMAGSWNMTKRRLREGVEFFRFLRSHEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LHV8</accession>
    <name>Q1LHV8_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Pyruvate-flavodoxin oxidoreductase</fullName>
            <ecNumber evidence="5">1.2.7.8</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">porG</name>
        <name evidence="5" type="ordered locus">Rmet_3396</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the pyruvate:ferredoxin/flavodoxin oxidoreductase family.</text>
    </comment>
    <dbReference type="EC" id="1.2.7.8" evidence="5"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10268.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517831.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3396"/>
    <dbReference type="EnsemblBacteria" id="ABF10268">
        <property type="protein sequence ID" value="ABF10268"/>
        <property type="gene ID" value="Rmet_3396"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3396"/>
    <dbReference type="eggNOG" id="COG1014">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG4231">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009166_1_0_4"/>
    <dbReference type="OMA" id="PRVVGKF"/>
    <dbReference type="OrthoDB" id="37279at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043805">
        <property type="term" value="F:indolepyruvate ferredoxin oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030976">
        <property type="term" value="F:thiamine pyrophosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07034">
        <property type="entry name" value="TPP_PYR_PFOR_IOR-alpha_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.920.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019752">
        <property type="entry name" value="Pyrv/ketoisovalerate_OxRed_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002880">
        <property type="entry name" value="Pyrv_Fd/Flavodoxin_OxRdtase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002869">
        <property type="entry name" value="Pyrv_flavodox_OxRed_cen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011766">
        <property type="entry name" value="TPP_enzyme-bd_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01558">
        <property type="entry name" value="POR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02775">
        <property type="entry name" value="TPP_enzyme_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="SSF52518"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53323">
        <property type="entry name" value="SSF53323"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TPP_enzyme_C" evidence="4">
        <location>
            <begin position="481"/>
            <end position="629"/>
        </location>
    </feature>
    <feature type="domain" description="POR" evidence="3">
        <location>
            <begin position="754"/>
            <end position="940"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01558"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF02775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABF10268.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="1197" mass="130759" checksum="41355A27330EB475" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LHW5</accession>
    <name>Q1LHW5_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">General secretion pathway protein L</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">gspL</name>
        <name evidence="4" type="ordered locus">Rmet_3389</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10261.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517824.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3389"/>
    <dbReference type="EnsemblBacteria" id="ABF10261">
        <property type="protein sequence ID" value="ABF10261"/>
        <property type="gene ID" value="Rmet_3389"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3389"/>
    <dbReference type="eggNOG" id="COG3297">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_577064_0_0_4"/>
    <dbReference type="OMA" id="WDLAQFD"/>
    <dbReference type="OrthoDB" id="1819441at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024230">
        <property type="entry name" value="GspL_cyto_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025691">
        <property type="entry name" value="GspL_pp_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12693">
        <property type="entry name" value="GspL_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05134">
        <property type="entry name" value="T2SSL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="SSF53067"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="T2SSL" evidence="2">
        <location>
            <begin position="5"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="domain" description="GspL_C" evidence="3">
        <location>
            <begin position="343"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="170"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="471"/>
            <end position="509"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF05134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF12693"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF10261.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="509" mass="54930" checksum="E2E74504DDF9B951" modified="2006-05-30" version="1">MSTTLYVRLPHRPHDQPQPWQFGAMPFALVRTPVGDKNRRQAAEQAPELLREGHAVPSEMPPADRLVLIVAASDVLLTAAMVPPLPPVRMRLALPNLVEDVLATDAAPCHIALGPAFDGAGSARGPRRRLLMVTDRAWMRAALDQFAEHKHRRRHVVAAQLCLPLAAPVTAPEPAPEPEPALATADGPVSPARAAAQSAPTEVGTVDTSPEQPATLVVEAATSTLAQSALLLDTAAPAPGELSRLWQLTVRTGPYEGYGLLLDDRALSTWQLLAPAGQWHGDNAALANAPVPALQGKRPPMHASRASETWRIWLAGIEECLQQPQLDLAQFEFAQGRMDRWNVRAWRWPALLVVALLLVQIVGMNVQWLTLRKEAKRLEAAQVDLLHTAFPNVPPVAEPILLMRRQVEQLRTASGRSTPNDFLPLADGFARAARDLPPDALLQLEYRAATLYVTLKPGTNTSALQNAVRQAGLQMDEDRSPPDAASRSGGAPAPTGSHWTVKAEGGTAS</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-01-15" modified="2022-05-25" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LI37</accession>
    <name>RL3_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">50S ribosomal protein L3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rplC</name>
        <name type="ordered locus">Rmet_3317</name>
    </gene>
    <organism>
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">One of the primary rRNA binding proteins, it binds directly near the 3'-end of the 23S rRNA, where it nucleates assembly of the 50S subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the 50S ribosomal subunit. Forms a cluster with proteins L14 and L19.</text>
    </comment>
    <comment type="PTM">
        <text evidence="1">Methylated by PrmB.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL3 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10189.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517768.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q1LI37"/>
    <dbReference type="SMR" id="Q1LI37"/>
    <dbReference type="STRING" id="266264.Rmet_3317"/>
    <dbReference type="EnsemblBacteria" id="ABF10189">
        <property type="protein sequence ID" value="ABF10189"/>
        <property type="gene ID" value="Rmet_3317"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3317"/>
    <dbReference type="eggNOG" id="COG0087">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_044142_4_1_4"/>
    <dbReference type="OMA" id="KRMAGRY"/>
    <dbReference type="OrthoDB" id="1270636at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01325_B">
        <property type="entry name" value="Ribosomal_L3_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000597">
        <property type="entry name" value="Ribosomal_L3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019927">
        <property type="entry name" value="Ribosomal_L3_bac/org-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019926">
        <property type="entry name" value="Ribosomal_L3_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11229">
        <property type="entry name" value="PTHR11229"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00297">
        <property type="entry name" value="Ribosomal_L3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="SSF50447"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03625">
        <property type="entry name" value="L3_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00474">
        <property type="entry name" value="RIBOSOMAL_L3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0488">Methylation</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <feature type="chain" id="PRO_1000052120" description="50S ribosomal protein L3">
        <location>
            <begin position="1"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="133"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="modified residue" description="N5-methylglutamine" evidence="1">
        <location>
            <position position="153"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="214" mass="22435" checksum="2E88BA7B65F0AD6B" modified="2006-05-30" version="1">MSLGLVGRKVGMTRIFTDDGDSIPVTVVEVGDNRVTQIKTDETDGYTAVQVTFGSRRASRVTKPLAGHLAKAGVEAGEVIKEFRIDAAKAAELQAGSSLSVDLFQVGQKIDVQGVSIGKGYAGTIKRHHFASGRATHGNSRSHNVPGSIGMAQDPGRVFPGKRMTGHLGDATRTVQNLEIAKIDAERKLLLVKGAIPGSKGGKVIVTPAVKAKA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-01-15" modified="2022-05-25" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LI42</accession>
    <name>RL22_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">50S ribosomal protein L22</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rplV</name>
        <name type="ordered locus">Rmet_3312</name>
    </gene>
    <organism>
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">This protein binds specifically to 23S rRNA; its binding is stimulated by other ribosomal proteins, e.g. L4, L17, and L20. It is important during the early stages of 50S assembly. It makes multiple contacts with different domains of the 23S rRNA in the assembled 50S subunit and ribosome.</text>
    </comment>
    <comment type="function">
        <text evidence="1">The globular domain of the protein is located near the polypeptide exit tunnel on the outside of the subunit, while an extended beta-hairpin is found that lines the wall of the exit tunnel in the center of the 70S ribosome.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the 50S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL22 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10184.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008642930.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q1LI42"/>
    <dbReference type="SMR" id="Q1LI42"/>
    <dbReference type="STRING" id="266264.Rmet_3312"/>
    <dbReference type="EnsemblBacteria" id="ABF10184">
        <property type="protein sequence ID" value="ABF10184"/>
        <property type="gene ID" value="Rmet_3312"/>
    </dbReference>
    <dbReference type="GeneID" id="60826605"/>
    <dbReference type="KEGG" id="rme:Rmet_3312"/>
    <dbReference type="eggNOG" id="COG0091">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_083987_3_3_4"/>
    <dbReference type="OMA" id="KRIQPRA"/>
    <dbReference type="OrthoDB" id="1666043at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015934">
        <property type="term" value="C:large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00336">
        <property type="entry name" value="Ribosomal_L22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.470.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01331_B">
        <property type="entry name" value="Ribosomal_L22_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001063">
        <property type="entry name" value="Ribosomal_L22"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018260">
        <property type="entry name" value="Ribosomal_L22/L17_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036394">
        <property type="entry name" value="Ribosomal_L22/L17_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005727">
        <property type="entry name" value="Ribosomal_L22_bac/chlpt-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13501">
        <property type="entry name" value="PTHR13501"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00237">
        <property type="entry name" value="Ribosomal_L22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54843">
        <property type="entry name" value="SSF54843"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01044">
        <property type="entry name" value="rplV_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00464">
        <property type="entry name" value="RIBOSOMAL_L22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <feature type="chain" id="PRO_1000052634" description="50S ribosomal protein L22">
        <location>
            <begin position="1"/>
            <end position="109"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01331"/>
        </source>
    </evidence>
    <sequence length="109" mass="11900" checksum="7A861F23E587F0D2" modified="2006-05-30" version="1">MEVKAIHRGARISAQKTRLVADQIRGLPIERALNILAFSPKKAAGIVKKVVESAIANAEHNEGADIDELKVKSIFVDKATSLKRFTARAKGRGNRIEKQTCHITVTLGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="92" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LIU2</accession>
    <name>Q1LIU2_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Thioredoxin-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Rmet_3062</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09934.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008645582.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3062"/>
    <dbReference type="EnsemblBacteria" id="ABF09934">
        <property type="protein sequence ID" value="ABF09934"/>
        <property type="gene ID" value="Rmet_3062"/>
    </dbReference>
    <dbReference type="GeneID" id="60820560"/>
    <dbReference type="KEGG" id="rme:Rmet_3062"/>
    <dbReference type="eggNOG" id="COG0526">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_042529_11_1_4"/>
    <dbReference type="OMA" id="RKMGRVH"/>
    <dbReference type="OrthoDB" id="1617952at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015036">
        <property type="term" value="F:disulfide oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017004">
        <property type="term" value="P:cytochrome complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013740">
        <property type="entry name" value="Redoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017937">
        <property type="entry name" value="Thioredoxin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08534">
        <property type="entry name" value="Redoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="SSF52833"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00194">
        <property type="entry name" value="THIOREDOXIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0201">Cytochrome c-type biogenesis</keyword>
    <keyword evidence="3" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="domain" description="Thioredoxin" evidence="6">
        <location>
            <begin position="33"/>
            <end position="180"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABF09934.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="180" mass="19190" checksum="76B79C95F7E24EDD" modified="2006-05-30" version="1">MTASAPTRRSPFVLWIIIAIVAAAAGALVAHFALAPKAVSDQAVETLFHTKMPDPAGAEVDLSKFRGKTLVVNFWAPWCGPCVEEMPELTALHGEYAPRNVEFVGIGIDSASNIQDFLKKVPVAYPLTVAGFAGTELSRTFGNTQGGLPFTVVITPDGTVKYRKMGRVHADELRAVLPGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LIX2</accession>
    <name>Q1LIX2_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">GCN5-related N-acetyltransferase, GNAT family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_3031</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09904.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517543.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3031"/>
    <dbReference type="EnsemblBacteria" id="ABF09904">
        <property type="protein sequence ID" value="ABF09904"/>
        <property type="gene ID" value="Rmet_3031"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3031"/>
    <dbReference type="eggNOG" id="COG3153">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_081840_2_0_4"/>
    <dbReference type="OMA" id="CDAFIPE"/>
    <dbReference type="OrthoDB" id="1687413at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008080">
        <property type="term" value="F:N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="SSF55729"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="1">
        <location>
            <begin position="3"/>
            <end position="154"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF09904.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="172" mass="18242" checksum="FF19ACC902BB4A5A" modified="2006-05-30" version="1">MTIQLRHETTNDIAAIEAVTIAAFADAPHTSHTEQFIVRALRDASELTLSIVAEEHGQVVGHVALSPVTITNDHGHKAKGWCGLGPISVLPQRQGHGIGSRLMEQALSELRAMQAEGCVLLGEPAYYGRFGFQAHAGLQLPGVPPGYFLALAFHGPVPEGIAQYSDAFNAAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="68" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LJ19</accession>
    <name>Q1LJ19_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_2984</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09857.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517518.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2984"/>
    <dbReference type="EnsemblBacteria" id="ABF09857">
        <property type="protein sequence ID" value="ABF09857"/>
        <property type="gene ID" value="Rmet_2984"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2984"/>
    <dbReference type="eggNOG" id="COG3753">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_084747_0_0_4"/>
    <dbReference type="OMA" id="GMQNGPH"/>
    <dbReference type="OrthoDB" id="1984170at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.690">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045372">
        <property type="entry name" value="YidB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027405">
        <property type="entry name" value="YidB-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20159">
        <property type="entry name" value="YidB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140804">
        <property type="entry name" value="SSF140804"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF09857.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="209" mass="18998" checksum="B0689A2D6284E473" modified="2006-05-30" version="1">MGLLDSVLGGVLGQLGGGQHGEQAGGGLNPKMMMALGLLAMMAMRHKGQEADGGAASAGADAGAGGLGGLGGLGGVLGGLLGGAGAAGGGASAAGGLDLGSLLGGLLGGQGGAGDSAQAMGAAAGGIGALRDVLAQAGLGQQVDSWIGTGANQAVSASDLSSALGGTGALQSLAEKTGLSADDVASSLSEGLPEVIDRLTPHGAVPPAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="99" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LJF4</accession>
    <name>Q1LJF4_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Peptidase M48, Ste24p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">yggG</name>
        <name evidence="10" type="ordered locus">Rmet_2849</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="6">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the peptidase M48 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09722.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="MEROPS" id="M48.022"/>
    <dbReference type="EnsemblBacteria" id="ABF09722">
        <property type="protein sequence ID" value="ABF09722"/>
        <property type="gene ID" value="Rmet_2849"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2849"/>
    <dbReference type="eggNOG" id="COG0501">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_074068_0_0_4"/>
    <dbReference type="OMA" id="IFLTRGF"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001915">
        <property type="entry name" value="Peptidase_M48"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01435">
        <property type="entry name" value="Peptidase_M48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 6" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="1 6" id="KW-0645">Protease</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <keyword evidence="4 6" id="KW-0862">Zinc</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193552" evidence="8">
        <location>
            <begin position="24"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase_M48" evidence="9">
        <location>
            <begin position="82"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="225"/>
            <end position="249"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ABF09722.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="249" mass="25971" checksum="3846F956E8F8FAFD" modified="2006-05-30" version="1">MIRLTATTAAVACAALLAGCAGANMDSLTQAGTSMFSAATLSDANVKQLSDQSCAQMDKQNTLATPDSTYSKRLVKIMSGLQNTGLPLDAKVYMTKDVNAWAMANGCVRVYSGLMDMMSDDEVRGVLGHEIGHVALGHTKSAMQVAYSTAAARGLLGSTGNATLTALSSSQLGALGEQLVNAQFSQRQESQADDYSFDLLTKNGANRQALVTAFQKLAKLDGGQSSMLSSHPGSAARAQHIQDRIASGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-10-07" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LJF9</accession>
    <name>Q1LJF9_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_2844</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09717.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF09717">
        <property type="protein sequence ID" value="ABF09717"/>
        <property type="gene ID" value="Rmet_2844"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2844"/>
    <dbReference type="HOGENOM" id="CLU_1204015_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF09717.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="230" mass="25598" checksum="CD1BBF0F4E014AC6" modified="2006-05-30" version="1">MGNTNTWKARLQDAGNGSVDRILELPDEVMAVMGWGIGDELELVPSGKGEIRLRKVCPAEESDYSRHLGVTYALLLQLREVTAERDANKLTEIASSIDWSAIASEFETVAGRYRSAVFRHGRRIIRNLRRPYMLYHDCTELAIQVDMLSSAIEDSPSLRATAEKALEEAYHAVLSRETLRGRPPQGAPAASPWPTLEALMEAAEDKRRRGHALQAAAHTGYLNGQRVFLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LJR9</accession>
    <name>Q1LJR9_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 6">dTDP-4-dehydrorhamnose reductase</fullName>
            <ecNumber evidence="4 6">1.1.1.133</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">rfbD</name>
        <name evidence="8" type="ordered locus">Rmet_2734</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Catalyzes the reduction of dTDP-6-deoxy-L-lyxo-4-hexulose to yield dTDP-L-rhamnose.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 6">
            <text>dTDP-beta-L-rhamnose + NADP(+) = dTDP-4-dehydro-beta-L-rhamnose + H(+) + NADPH</text>
            <dbReference type="Rhea" id="RHEA:21796"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57510"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:62830"/>
            <dbReference type="EC" id="1.1.1.133"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="6">Binds 1 Mg(2+) ion per monomer.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 6">Carbohydrate biosynthesis; dTDP-L-rhamnose biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 6">Belongs to the dTDP-4-dehydrorhamnose reductase family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.133" evidence="4 6"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09607.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517308.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2734"/>
    <dbReference type="EnsemblBacteria" id="ABF09607">
        <property type="protein sequence ID" value="ABF09607"/>
        <property type="gene ID" value="Rmet_2734"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2734"/>
    <dbReference type="eggNOG" id="COG1091">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045518_1_0_4"/>
    <dbReference type="OMA" id="AGETTWH"/>
    <dbReference type="OrthoDB" id="1076083at2"/>
    <dbReference type="UniPathway" id="UPA00124"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008831">
        <property type="term" value="F:dTDP-4-dehydrorhamnose reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019305">
        <property type="term" value="P:dTDP-rhamnose biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005913">
        <property type="entry name" value="dTDP_dehydrorham_reduct"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029903">
        <property type="entry name" value="RmlD-like-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10491">
        <property type="entry name" value="PTHR10491"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04321">
        <property type="entry name" value="RmlD_sub_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01214">
        <property type="entry name" value="rmlD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0521">NADP</keyword>
    <keyword evidence="6 8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RmlD_sub_bind" evidence="7">
        <location>
            <begin position="10"/>
            <end position="304"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000079"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010944"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00017099"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU364082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF04321"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ABF09607.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="306" mass="32881" checksum="C867697C32E84BAA" modified="2006-05-30" version="1">MLREAQRAPTLLVTGSNGQVGFELRRSLAPLGRVVALDRSQFDLTRPDALRELVRSIRPDVIVNPAAYTAVDKAETEAEAAFAVNGVAPGVLAEEARRLGSLFVHYSTDYVFDGTKDGAYLEGDAVNPQSVYGKSKLAGEQVVAATGAASLVLRTCWVAGAHGGNFAKTMLRLARERDSLRVIADQFGAPTTASLIADVTAQIVARHWLHAPGREAFPTGVYHLAAGGETTWHGYATHVLSRAAAKGVEMKVDPTRIEPIPASQYPLPAPRPANSRLDTGKLRDTFNIHLPDWAEGIDLLLDQILS</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LJT8</accession>
    <name>Q1LJT8_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">ADP-heptose:LPS heptosyl transferase I</fullName>
            <ecNumber evidence="3">2.-.-.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">rfaC</name>
        <name evidence="3" type="ordered locus">Rmet_2715</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.-.-.-" evidence="3"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09588.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2715"/>
    <dbReference type="CAZy" id="GT9">
        <property type="family name" value="Glycosyltransferase Family 9"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF09588">
        <property type="protein sequence ID" value="ABF09588"/>
        <property type="gene ID" value="Rmet_2715"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2715"/>
    <dbReference type="eggNOG" id="COG0859">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038371_6_0_4"/>
    <dbReference type="OMA" id="ITHLAWA"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008920">
        <property type="term" value="F:lipopolysaccharide heptosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009244">
        <property type="term" value="P:lipopolysaccharide core region biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03789">
        <property type="entry name" value="GT9_LPS_heptosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002201">
        <property type="entry name" value="Glyco_trans_9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011908">
        <property type="entry name" value="LipoPS_heptosylTferase-I"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01075">
        <property type="entry name" value="Glyco_transf_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02193">
        <property type="entry name" value="heptsyl_trn_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 3" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF09588.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="363" mass="39736" checksum="59ED26F27B6E7BFF" modified="2006-05-30" version="1">MRSSRSDVVQVSNQETPSPQAAVSPFGLPARPRILIVKVSSLGDVVHNMPLVHDLRARWPDAEIDWVVEEGYVDLVRLLPDVGRVIPFALRRWRKRFWRGETWAEIGALRDAIRARPYDAVLETQGLLKTAIVARTAARVAGAPVIGLGNATQGSGYEPAARLFYTAPVKVPRQTHSVRRSRLLGAALTGITPPEPPRFFGPAANTLSVDDPLWADLPARYAVCFHATAGAKKRWAVENWHALGRRLEAEGLTVLLPWGSEAERRDAEALAAGIPGARVLPRFSVMQGFGLINRAEVVIGVDTGLVHIAAALCRPTVEIYTATWRWKTEGYWSDRIANVGDDGIVPTVDEVHDAAIRVRGVTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LJY2</accession>
    <name>Q1LJY2_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Putative yeeE-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">Rmet_2671</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the UPF0394 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09544.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517246.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2671"/>
    <dbReference type="EnsemblBacteria" id="ABF09544">
        <property type="protein sequence ID" value="ABF09544"/>
        <property type="gene ID" value="Rmet_2671"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2671"/>
    <dbReference type="eggNOG" id="COG2391">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_050656_2_0_4"/>
    <dbReference type="OMA" id="THFCTMG"/>
    <dbReference type="OrthoDB" id="1899767at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007272">
        <property type="entry name" value="Sulf_transp"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04143">
        <property type="entry name" value="Sulf_transp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="6"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="54"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="86"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="121"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="178"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="203"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="273"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="314"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="341"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="domain" description="Sulf_transp" evidence="8">
        <location>
            <begin position="30"/>
            <end position="358"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009590"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF04143"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ABF09544.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="371" mass="39046" checksum="B0056776B725E9C9" modified="2006-05-30" version="1">MPDIDIPALMRTVLLSTFVLTFLFGAILQRTHFCTMGAVSDIVNIGDWSRMRMWIMAIGVAMIGTGVLAWAGLIDPTKTIYTSARLGWLSTAVGGLMFGFGMVLASGCGSKTLVRIGAGNLKSLVVFVFLGLSAYMTLRGLFGVVRVSTVDTVAVTLPATQDLPSLIAHGTGLARGTLQLALGVVIGAVLVIWALLGNGFRTFDNLLGGLAVGLIIVGMWYVSGHIGYVSEDPNTLEELFVASNSGRMESLSFVAPYAYTLDWLMMFSDKSKVLTIGIVSVFGVIAGAAAYALVSRTFRWEGFGNAEDVANHMVGGILMGAGGVTALGCTVGQGLSGVSTLAVGSFIAFAAIIVGAVLAFKYQIWRLERMA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-01-15" modified="2022-08-03" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LK81</accession>
    <name>TYSY_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Thymidylate synthase</fullName>
            <shortName evidence="1">TS</shortName>
            <shortName evidence="1">TSase</shortName>
            <ecNumber evidence="1">2.1.1.45</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">thyA</name>
        <name type="ordered locus">Rmet_2568</name>
    </gene>
    <organism>
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the reductive methylation of 2'-deoxyuridine-5'-monophosphate (dUMP) to 2'-deoxythymidine-5'-monophosphate (dTMP) while utilizing 5,10-methylenetetrahydrofolate (mTHF) as the methyl donor and reductant in the reaction, yielding dihydrofolate (DHF) as a by-product. This enzymatic reaction provides an intracellular de novo source of dTMP, an essential precursor for DNA biosynthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(6R)-5,10-methylene-5,6,7,8-tetrahydrofolate + dUMP = 7,8-dihydrofolate + dTMP</text>
            <dbReference type="Rhea" id="RHEA:12104"/>
            <dbReference type="ChEBI" id="CHEBI:15636"/>
            <dbReference type="ChEBI" id="CHEBI:57451"/>
            <dbReference type="ChEBI" id="CHEBI:63528"/>
            <dbReference type="ChEBI" id="CHEBI:246422"/>
            <dbReference type="EC" id="2.1.1.45"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Pyrimidine metabolism; dTTP biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the thymidylate synthase family. Bacterial-type ThyA subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.45" evidence="1"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09445.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517148.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q1LK81"/>
    <dbReference type="SMR" id="Q1LK81"/>
    <dbReference type="STRING" id="266264.Rmet_2568"/>
    <dbReference type="EnsemblBacteria" id="ABF09445">
        <property type="protein sequence ID" value="ABF09445"/>
        <property type="gene ID" value="Rmet_2568"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2568"/>
    <dbReference type="eggNOG" id="COG0207">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021669_0_0_4"/>
    <dbReference type="OMA" id="KQYLDLC"/>
    <dbReference type="OrthoDB" id="1212177at2"/>
    <dbReference type="UniPathway" id="UPA00575"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004799">
        <property type="term" value="F:thymidylate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006231">
        <property type="term" value="P:dTMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006235">
        <property type="term" value="P:dTTP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00351">
        <property type="entry name" value="TS_Pyrimidine_HMase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.572.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00008">
        <property type="entry name" value="Thymidy_synth_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045097">
        <property type="entry name" value="Thymidate_synth/dCMP_Mease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023451">
        <property type="entry name" value="Thymidate_synth/dCMP_Mease_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036926">
        <property type="entry name" value="Thymidate_synth/dCMP_Mease_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000398">
        <property type="entry name" value="Thymidylate_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020940">
        <property type="entry name" value="Thymidylate_synthase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11548">
        <property type="entry name" value="PTHR11548"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00303">
        <property type="entry name" value="Thymidylat_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00108">
        <property type="entry name" value="THYMDSNTHASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55831">
        <property type="entry name" value="SSF55831"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03284">
        <property type="entry name" value="thym_sym"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00091">
        <property type="entry name" value="THYMIDYLATE_SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0489">Methyltransferase</keyword>
    <keyword id="KW-0545">Nucleotide biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_1000000656" description="Thymidylate synthase">
        <location>
            <begin position="1"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="1">
        <location>
            <position position="146"/>
        </location>
    </feature>
    <feature type="binding site" description="in other chain" evidence="1">
        <location>
            <position position="21"/>
        </location>
        <ligand>
            <name>dUMP</name>
            <dbReference type="ChEBI" id="CHEBI:246422"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="51"/>
        </location>
        <ligand>
            <name>(6R)-5,10-methylene-5,6,7,8-tetrahydrofolate</name>
            <dbReference type="ChEBI" id="CHEBI:15636"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="126"/>
            <end position="127"/>
        </location>
        <ligand>
            <name>dUMP</name>
            <dbReference type="ChEBI" id="CHEBI:246422"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="1">
        <location>
            <begin position="166"/>
            <end position="169"/>
        </location>
        <ligand>
            <name>dUMP</name>
            <dbReference type="ChEBI" id="CHEBI:246422"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="169"/>
        </location>
        <ligand>
            <name>(6R)-5,10-methylene-5,6,7,8-tetrahydrofolate</name>
            <dbReference type="ChEBI" id="CHEBI:15636"/>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="1">
        <location>
            <position position="177"/>
        </location>
        <ligand>
            <name>dUMP</name>
            <dbReference type="ChEBI" id="CHEBI:246422"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="1">
        <location>
            <begin position="207"/>
            <end position="209"/>
        </location>
        <ligand>
            <name>dUMP</name>
            <dbReference type="ChEBI" id="CHEBI:246422"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="263"/>
        </location>
        <ligand>
            <name>(6R)-5,10-methylene-5,6,7,8-tetrahydrofolate</name>
            <dbReference type="ChEBI" id="CHEBI:15636"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00008"/>
        </source>
    </evidence>
    <sequence length="264" mass="30509" checksum="BA01DF8DC547D57C" modified="2006-05-30" version="1">MKQYLDFMRHVYEHGTEKSDRTGTGTRSVFGYQMRFDLNEGFPVVTTKKLHLKSIIHELLWFLQGSTNIKYLKDNGVTIWDEWADANGELGPIYGYQWRAWPTPDGRHIDQITEVVQQIRDNPDSRRLIVSAWNVGEIPQMKLPPCHAFFQFYVADGKLSCQLYQRSADIFLGVPFNIASYALLTHMIAQQCDLGVGDFVWTGGDCHIYSNHFEQVETQLSREPMKLPTLRIKHRPDSIFDYKYDDFELVGYESHPAIKAPVAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="107" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LKC5</accession>
    <name>Q1LKC5_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative transcriptional regulator, LuxR-family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_2524</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09401.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2524"/>
    <dbReference type="EnsemblBacteria" id="ABF09401">
        <property type="protein sequence ID" value="ABF09401"/>
        <property type="gene ID" value="Rmet_2524"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2524"/>
    <dbReference type="eggNOG" id="COG2909">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006325_3_1_4"/>
    <dbReference type="OMA" id="SITERTC"/>
    <dbReference type="OrthoDB" id="1377603at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06170">
        <property type="entry name" value="LuxR_C_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016032">
        <property type="entry name" value="Sig_transdc_resp-reg_C-effctor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041617">
        <property type="entry name" value="TPR_MalT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000792">
        <property type="entry name" value="Tscrpt_reg_LuxR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13401">
        <property type="entry name" value="AAA_22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00196">
        <property type="entry name" value="GerE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17874">
        <property type="entry name" value="TPR_MalT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00038">
        <property type="entry name" value="HTHLUXR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00421">
        <property type="entry name" value="HTH_LUXR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46894">
        <property type="entry name" value="SSF46894"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50043">
        <property type="entry name" value="HTH_LUXR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH luxR-type" evidence="2">
        <location>
            <begin position="845"/>
            <end position="910"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF09401.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="914" mass="99373" checksum="FC6BACB1D7FA5968" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LKD5</accession>
    <name>Q1LKD5_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Glutathione S-transferase</fullName>
            <ecNumber evidence="3">2.5.1.18</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">h</name>
        <name evidence="3" type="ordered locus">Rmet_2514</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.5.1.18" evidence="3"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09391.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517089.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2514"/>
    <dbReference type="EnsemblBacteria" id="ABF09391">
        <property type="protein sequence ID" value="ABF09391"/>
        <property type="gene ID" value="Rmet_2514"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2514"/>
    <dbReference type="eggNOG" id="COG0625">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011226_6_2_4"/>
    <dbReference type="OMA" id="QKVVWCA"/>
    <dbReference type="OrthoDB" id="1752966at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004364">
        <property type="term" value="F:glutathione transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006749">
        <property type="term" value="P:glutathione metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010987">
        <property type="entry name" value="Glutathione-S-Trfase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036282">
        <property type="entry name" value="Glutathione-S-Trfase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004045">
        <property type="entry name" value="Glutathione_S-Trfase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004046">
        <property type="entry name" value="GST_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00043">
        <property type="entry name" value="GST_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13417">
        <property type="entry name" value="GST_N_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47616">
        <property type="entry name" value="SSF47616"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="SSF52833"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50405">
        <property type="entry name" value="GST_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50404">
        <property type="entry name" value="GST_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="GST N-terminal" evidence="1">
        <location>
            <begin position="1"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="domain" description="GST C-terminal" evidence="2">
        <location>
            <begin position="94"/>
            <end position="214"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF09391.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="214" mass="24450" checksum="1B5FED3C183F15E3" modified="2006-05-30" version="1">MLRIWGRLSSINVQKVVWCARELHLDHERIDIGNQKGELDTEAYVRLNPNRLIPVIEDFRDTDVAGEPFVLWESNAIVRYLCARYGEDTLWPSDVKARASADRWMDWQTTTFSPAMVQAFLQLVRTPADKRDEAVIEKSCERTEPLAKMLDDALEGREFIGGDRFTMADVSLACAAHRWMGMPKAHTARPNLERWLAAMRARPAATGILELPLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LKF1</accession>
    <name>Q1LKF1_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_2498</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09375.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517075.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2498"/>
    <dbReference type="EnsemblBacteria" id="ABF09375">
        <property type="protein sequence ID" value="ABF09375"/>
        <property type="gene ID" value="Rmet_2498"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2498"/>
    <dbReference type="eggNOG" id="COG4700">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_088596_0_0_4"/>
    <dbReference type="OMA" id="DHNREWL"/>
    <dbReference type="OrthoDB" id="1525278at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014562">
        <property type="entry name" value="UCP030959_TPR_rpt-cont"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF030959">
        <property type="entry name" value="UCP030959"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="SSF48452"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="31"/>
            <end position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF09375.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="253" mass="27849" checksum="594C307F0BA6749E" modified="2006-05-30" version="1">MSNLGIGGIGLHVIVAIFFAVHAVRTHQNMYWLLLLFLFPGLGSIVYFFAVYLPSLRDSRGARAASRAIKQIVDPNRAVREAREAFDRAPTVDHRMRLGAALLEVGNAAEALEHYQAAANGPFASDPALLEGLARAQVATGRHADAQATLEKLFAAHPQARDQAEPALLYARALSALDAPGTRDAFERSLTCASDAAPRCLYADWLSRQGDAADRERAKSLYSEIVHDARHWPRHAREHNREWLQRAQVALGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="98" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LKG1</accession>
    <name>Q1LKG1_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Transcriptional regulator, GntR-family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Rmet_2488</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09365.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008649351.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2488"/>
    <dbReference type="EnsemblBacteria" id="ABF09365">
        <property type="protein sequence ID" value="ABF09365"/>
        <property type="gene ID" value="Rmet_2488"/>
    </dbReference>
    <dbReference type="GeneID" id="60821037"/>
    <dbReference type="KEGG" id="rme:Rmet_2488"/>
    <dbReference type="eggNOG" id="COG2188">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_063236_3_0_4"/>
    <dbReference type="OMA" id="SRYSFEF"/>
    <dbReference type="OrthoDB" id="1775418at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006744">
        <property type="term" value="P:ubiquinone biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd07377">
        <property type="entry name" value="WHTH_GntR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1410.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028978">
        <property type="entry name" value="Chorismate_lyase_/UTRA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000524">
        <property type="entry name" value="Tscrpt_reg_HTH_GntR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011663">
        <property type="entry name" value="UTRA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00392">
        <property type="entry name" value="GntR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07702">
        <property type="entry name" value="UTRA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00035">
        <property type="entry name" value="HTHGNTR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00345">
        <property type="entry name" value="HTH_GNTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00866">
        <property type="entry name" value="UTRA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64288">
        <property type="entry name" value="SSF64288"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50949">
        <property type="entry name" value="HTH_GNTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0456">Lyase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0831">Ubiquinone biosynthesis</keyword>
    <feature type="domain" description="HTH gntR-type" evidence="4">
        <location>
            <begin position="51"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="21"/>
            <end position="36"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABF09365.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="284" mass="30911" checksum="FEE119B09B0E16E1" modified="2006-05-30" version="1">MSSLPTSLPAAAANGAPESGAPQSGAGTQPSTNAPGAAVAPATAAPSPTFSPLYQQIKALIMQSLQTGEWKPGEMIPSEMDLAARYKVSQGTVRKAIDELAAENLVARRQGKGTFVTTHYEDVVKFRFLRLVPDEGEPHYGSSRVLECKRLRAPAEIARLLDIRTGDSVVQIRRVLTFSNEATVLDEIWLLGANFKGLTAEKLNEWKGPMYALFEGEFGTRMIRATEKIRAVVADDTAAELLSVAPGSPLLSVERVSFTYGDRPVEVRRGLYVTTRHYYQNDLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LKH9</accession>
    <name>Q1LKH9_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Type IV-pili assembly fimV-related transmembrane protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_2470</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09347.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517060.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2470"/>
    <dbReference type="EnsemblBacteria" id="ABF09347">
        <property type="protein sequence ID" value="ABF09347"/>
        <property type="gene ID" value="Rmet_2470"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2470"/>
    <dbReference type="eggNOG" id="COG3170">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007099_0_0_4"/>
    <dbReference type="OMA" id="QHSVFGA"/>
    <dbReference type="OrthoDB" id="595000at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00118">
        <property type="entry name" value="LysM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.2200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.350.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038440">
        <property type="entry name" value="FimV_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018392">
        <property type="entry name" value="LysM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036779">
        <property type="entry name" value="LysM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020011">
        <property type="entry name" value="Motility_prot_FimV_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020012">
        <property type="entry name" value="Motility_prot_FimV_N"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03505">
        <property type="entry name" value="FimV_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03504">
        <property type="entry name" value="FimV_Cterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="164"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="321"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="421"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="168"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="328"/>
            <end position="342"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF09347.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="940" mass="97685" checksum="D1E40228DE370EC9" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LKL0</accession>
    <name>Q1LKL0_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Pseudouridine synthase</fullName>
            <ecNumber evidence="3">5.4.99.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">rluC</name>
        <name evidence="5" type="ordered locus">Rmet_2439</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Responsible for synthesis of pseudouridine from uracil.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>a uridine in RNA = a pseudouridine in RNA</text>
            <dbReference type="Rhea" id="RHEA:48348"/>
            <dbReference type="Rhea" id="RHEA-COMP:12068"/>
            <dbReference type="Rhea" id="RHEA-COMP:12069"/>
            <dbReference type="ChEBI" id="CHEBI:65314"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the pseudouridine synthase RluA family.</text>
    </comment>
    <dbReference type="EC" id="5.4.99.-" evidence="3"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09316.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2439"/>
    <dbReference type="EnsemblBacteria" id="ABF09316">
        <property type="protein sequence ID" value="ABF09316"/>
        <property type="gene ID" value="Rmet_2439"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2439"/>
    <dbReference type="eggNOG" id="COG0564">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016902_1_1_4"/>
    <dbReference type="OMA" id="PKTHVYR"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120159">
        <property type="term" value="F:rRNA pseudouridine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000455">
        <property type="term" value="P:enzyme-directed rRNA pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="CDD" id="cd02869">
        <property type="entry name" value="PseudoU_synth_RluA_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00165">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.290.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020103">
        <property type="entry name" value="PsdUridine_synth_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006225">
        <property type="entry name" value="PsdUridine_synth_RluC/D"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006224">
        <property type="entry name" value="PsdUridine_synth_RluC/D_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006145">
        <property type="entry name" value="PsdUridine_synth_RsuA/RluA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002942">
        <property type="entry name" value="S4_RNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036986">
        <property type="entry name" value="S4_RNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00849">
        <property type="entry name" value="PseudoU_synth_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01479">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00363">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55120">
        <property type="entry name" value="SSF55120"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00005">
        <property type="entry name" value="rluA_subfam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01129">
        <property type="entry name" value="PSI_RLU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0413">Isomerase</keyword>
    <keyword evidence="5" id="KW-0456">Lyase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="S4" evidence="4">
        <location>
            <begin position="35"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="active site" evidence="2">
        <location>
            <position position="155"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010876"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR606225-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU362028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABF09316.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="338" mass="37474" checksum="61F32D036E0C8138" modified="2006-05-30" version="1">MKMNELRHQIATEPQSRPSGLQVAYVTVDESSEGQRIDNFLLKVAKGVPKSHIYRVLRSGEVRVNKGRVDATYRLQNGDVVRIPPMRVAEKAAPTGGFVPAGEFPVLFEDAALLVIDKPAGVAVHGGSGVAFGVIEQLRRARPEAKFLELVHRLDRETSGILVLAKKRSVLVHLHEQIRGNGMDKRYFACVAGEFPNTRQHVKLPLYKYSTPDGERRVRVQADGLASHTVFNRMEAFDGYTLLEAELKTGRTHQIRVHLQSIGFPIVGDDKYGDFALNKALSRSGTKPGLKRMFLHAHRLTLTHPVTGAPMTVSAPLPAECVEFLQQLRQGRPARDAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LKM6</accession>
    <name>Q1LKM6_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Anti-sigma factor, negative regulator of sigma24 activity</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">rseB</name>
        <name evidence="8" type="ordered locus">Rmet_2423</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Periplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the RseB family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09300.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517019.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2423"/>
    <dbReference type="EnsemblBacteria" id="ABF09300">
        <property type="protein sequence ID" value="ABF09300"/>
        <property type="gene ID" value="Rmet_2423"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2423"/>
    <dbReference type="eggNOG" id="COG3026">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_054710_0_0_4"/>
    <dbReference type="OMA" id="DDFRYQY"/>
    <dbReference type="OrthoDB" id="1490766at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042597">
        <property type="term" value="C:periplasmic space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd16327">
        <property type="entry name" value="RseB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033436">
        <property type="entry name" value="MucB/RseB_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038484">
        <property type="entry name" value="MucB/RseB_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033434">
        <property type="entry name" value="MucB/RseB_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005588">
        <property type="entry name" value="MucB_RseB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38782">
        <property type="entry name" value="PTHR38782"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03888">
        <property type="entry name" value="MucB_RseB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17188">
        <property type="entry name" value="MucB_RseB_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005427">
        <property type="entry name" value="RseB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0574">Periplasm</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193530" evidence="5">
        <location>
            <begin position="37"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="domain" description="MucB_RseB" evidence="6">
        <location>
            <begin position="47"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="domain" description="MucB_RseB_C" evidence="7">
        <location>
            <begin position="244"/>
            <end position="351"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022764"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF03888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF17188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ABF09300.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="359" mass="39686" checksum="3D452EFEE97B8240" modified="2006-05-30" version="1">MHKGPSLANAAGVHGKKGLRRSFFLALCLTAAAATAQPSDSTLNRRDATAWLNKIHRAAQRENYVGTLIYQRGSIIHASRIQHYSDLVHNEYERLETLDGKPREVLRQNDVVHSLIPEAKLVVVEKQEAKDRFPALLATNKSDVLDLYDMHKLPAERVAGVECEVFSLDPHDTARYAVRLWADRNSGLLMRAQTIGEGGKVLEQVAFSQVDIGVPSDKQRILNAIKGASSWSHYEVTYQPASIADEGWSINTPIKGFQKIREVRRPLGELHAPTSANKSGQVFEVLQVVYSDGLTGLSVFIEPVSEQRARREGVASLGATQVVVRRVADFWITVVGEVPASTVRQFAAGVEYRPPKAVH</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-10-07" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LLE9</accession>
    <name>Q1LLE9_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Transcriptional regulator, LysR-family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_2148</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the LysR transcriptional regulatory family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09027.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2148"/>
    <dbReference type="EnsemblBacteria" id="ABF09027">
        <property type="protein sequence ID" value="ABF09027"/>
        <property type="gene ID" value="Rmet_2148"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2148"/>
    <dbReference type="eggNOG" id="COG0583">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039613_6_0_4"/>
    <dbReference type="OMA" id="DEFYTHY"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005119">
        <property type="entry name" value="LysR_subst-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000847">
        <property type="entry name" value="Tscrpt_reg_HTH_LysR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00126">
        <property type="entry name" value="HTH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03466">
        <property type="entry name" value="LysR_substrate"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00039">
        <property type="entry name" value="HTHLYSR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50931">
        <property type="entry name" value="HTH_LYSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH lysR-type" evidence="2">
        <location>
            <begin position="22"/>
            <end position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF09027.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="317" mass="34676" checksum="D6C142E3FCFC7F19" modified="2006-05-30" version="1">MRCGPIIQVQISYRSNAIYAYMNLKRLEHLVAVAEEGSLAAAARRVHLSQPALTRSIQTLEEEAGAVLFDRGTRGVTLTAVGRMVTERARRILFETHCLTRDLTLVRQHEIGSVQIGLGAFPAAMLLPEVLCLLHREWPGLQIATEVSDPRALLDALRAERLDFVVIEQSTVPTLAELEVQRLPEESAGLFVRQGHPLARRPVLLPALREAALVSVVFPPETHERLRKVLRCKPGEALPFQVESNDFRALTYLAKHTDAVLLAPARAVRDETAAGSLVPLDVPGLPAMTMQFAVVRLAQRTLSPAAGRAVAAIEAVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LLL7</accession>
    <name>Q1LLL7_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Cytochrome d1, heme region</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">nirF</name>
        <name evidence="2" type="ordered locus">Rmet_2080</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08959.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516793.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2080"/>
    <dbReference type="EnsemblBacteria" id="ABF08959">
        <property type="protein sequence ID" value="ABF08959"/>
        <property type="gene ID" value="Rmet_2080"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2080"/>
    <dbReference type="eggNOG" id="COG3391">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_040920_0_0_4"/>
    <dbReference type="OMA" id="KMPHLRG"/>
    <dbReference type="OrthoDB" id="302683at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.140.10.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003143">
        <property type="entry name" value="Cyt_cd1_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011048">
        <property type="entry name" value="Haem_d1_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51004">
        <property type="entry name" value="SSF51004"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193196" evidence="1">
        <location>
            <begin position="23"/>
            <end position="390"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF08959.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="390" mass="41923" checksum="1636ABC399CC99C9" modified="2006-05-30" version="1">MRRTALPVVLALSALLAGCATAVRGTGDMGVVVERAAGRIQVVETSGMTRLATIDGFGDLSHASVVFARDGRYAYVFGRDGGLTRVDLLNAKVTHRTIQAGNSIGGAITQDGRVVAAQNYEPGGIRLFDAATLEPLADIPAIGPDGRRSKVVGLADLPGKRLAASLFEAGEIWIIDVADPRHPKVTKLPAGKQPYDGLVTPDGRWYLAGLFGEDGLALVDLWQSPPAARHVLSGYGRGSAPLPVYKMPHLRGWAMAQGKAWLPAIGRHEVLIADTANGWQEAGRVEVAGQPVFVMAQPDGRQVWVNFAFPNNETVQVIDTATRKVVRTLKPCRGVLHMEFTPKGEAVWLSCRDDNRVVVYDTTSYEPLATLPADHPSGIFFTSRAQRFGM</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LLS1</accession>
    <name>Q1LLS1_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">RND efflux system (NodT family)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Rmet_2026</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the outer membrane factor (OMF) (TC 1.B.17) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08905.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2026"/>
    <dbReference type="EnsemblBacteria" id="ABF08905">
        <property type="protein sequence ID" value="ABF08905"/>
        <property type="gene ID" value="Rmet_2026"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2026"/>
    <dbReference type="eggNOG" id="COG1538">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012817_13_2_4"/>
    <dbReference type="OMA" id="PRVDANY"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015562">
        <property type="term" value="F:efflux transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003423">
        <property type="entry name" value="OMP_efflux"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010131">
        <property type="entry name" value="RND_efflux_OM_lipoprot_NodT"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02321">
        <property type="entry name" value="OEP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01845">
        <property type="entry name" value="outer_NodT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0564">Palmitate</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1134">Transmembrane beta strand</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="260"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="12"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU362097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABF08905.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="519" mass="56084" checksum="1D575A8F5589298A" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LM16</accession>
    <name>Q1LM16_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">N-carbamoyl-L-amino-acid hydrolase</fullName>
            <ecNumber evidence="5">3.5.1.87</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">amaB-2</name>
        <name evidence="5" type="ordered locus">Rmet_1931</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="3">Binds 2 Zn(2+) ions per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase M20 family.</text>
    </comment>
    <dbReference type="EC" id="3.5.1.87" evidence="5"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08810.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516662.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1931"/>
    <dbReference type="EnsemblBacteria" id="ABF08810">
        <property type="protein sequence ID" value="ABF08810"/>
        <property type="gene ID" value="Rmet_1931"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1931"/>
    <dbReference type="eggNOG" id="COG0624">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024588_2_1_4"/>
    <dbReference type="OMA" id="CLGQKWF"/>
    <dbReference type="OrthoDB" id="829830at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016813">
        <property type="term" value="F:hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amidines"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050538">
        <property type="term" value="F:N-carbamoyl-L-amino-acid hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010158">
        <property type="entry name" value="Amidase_Cbmase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036264">
        <property type="entry name" value="Bact_exopeptidase_dim_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002933">
        <property type="entry name" value="Peptidase_M20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011650">
        <property type="entry name" value="Peptidase_M20_dimer"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32494">
        <property type="entry name" value="PTHR32494"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07687">
        <property type="entry name" value="M20_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01546">
        <property type="entry name" value="Peptidase_M20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001235">
        <property type="entry name" value="Amidase_carbamoylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55031">
        <property type="entry name" value="SSF55031"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01879">
        <property type="entry name" value="hydantase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="M20_dimer" evidence="4">
        <location>
            <begin position="218"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="86"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="97"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="132"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="196"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="388"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006153"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR001235-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF07687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABF08810.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="415" mass="43994" checksum="568A8C9DC2C4B1E6" modified="2006-05-30" version="1">MDSQFTPRINGARLWQSLMDLGALGATPKGGVCRIALTEDDRKGRDLVASWCREAGLDVRVDEIGNVFARRAGTDPTAPAVATGSHIDTQPSGGKFDGNFGVLAGLEVMRTLNDLGIATRAPLEVAIWTNEEGTRFTPVMMGSGVFAGVFDAEFARRQQDRDGVSVGDALAAIGYRGTQRAGEVPGGMYSAYFEAHIEQGPVLEAAGLPIGVVSGALGQQWYDVTVTGQDAHAGPTPLALRHDALLASARMIEAVNRIARDEAPHGRGTVGFLEVTPNSRNVIPGRVDFSVDVRHLSQDGLDRMDAAMRGAFAGIADAAAVDVEIRQVVKFEPCLFAPACVDSVRRAAVGLDLPHMDVVSGAGHDAVYVAQRAPTGMIFVPCKDGISHNELEDALPEHIEAGANVLLRAMLEHAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LMA8</accession>
    <name>Q1LMA8_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Hydroxypyruvate isomerase</fullName>
            <ecNumber evidence="4">5.3.1.22</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">hyi1</name>
        <name evidence="4" type="ordered locus">Rmet_1839</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the hyi family.</text>
    </comment>
    <dbReference type="EC" id="5.3.1.22" evidence="4"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08718.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516569.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1839"/>
    <dbReference type="EnsemblBacteria" id="ABF08718">
        <property type="protein sequence ID" value="ABF08718"/>
        <property type="gene ID" value="Rmet_1839"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1839"/>
    <dbReference type="eggNOG" id="COG3622">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_050006_1_2_4"/>
    <dbReference type="OMA" id="CEYRPRA"/>
    <dbReference type="OrthoDB" id="1007505at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008903">
        <property type="term" value="F:hydroxypyruvate isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026040">
        <property type="entry name" value="HyI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036237">
        <property type="entry name" value="Xyl_isomerase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013022">
        <property type="entry name" value="Xyl_isomerase-like_TIM-brl"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01261">
        <property type="entry name" value="AP_endonuc_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006241">
        <property type="entry name" value="HyI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51658">
        <property type="entry name" value="SSF51658"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 4" id="KW-0413">Isomerase</keyword>
    <keyword evidence="4" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AP_endonuc_2" evidence="3">
        <location>
            <begin position="21"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="2">
        <location>
            <position position="143"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="2">
        <location>
            <position position="240"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRNR" id="PIRNR006241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR006241-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF08718.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="260" mass="28967" checksum="3671B9B6C383C40D" modified="2006-05-30" version="1">MPRFAANLSMMYTEHDFLDRFAAAAKDGFQAVEYLFPYDHPAAEIRKRLDDNGLTQALFNAPPGDWGAGERGIAALPGRETEFRTEFAKALEYAAVLGNDRVHVMAGIVPADADHARCRSIYLENLAYASASAASHGITVLIEPINTRDMPGYFLNRQDEGQTICRQVGAANLKVQFDCYHCQIVEGDIAKKLERDFAGIGHIQIAGVPERHEPDIGELNYPYLFDVIDRLGYTGWIGCEYRPRGGTSEGLGWIKPWLKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LME7</accession>
    <name>Q1LME7_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative transcriptional regulator, MarR family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_1800</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08679.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516529.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1800"/>
    <dbReference type="EnsemblBacteria" id="ABF08679">
        <property type="protein sequence ID" value="ABF08679"/>
        <property type="gene ID" value="Rmet_1800"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1800"/>
    <dbReference type="eggNOG" id="COG1846">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_083287_8_0_4"/>
    <dbReference type="OMA" id="RREWGMI"/>
    <dbReference type="OrthoDB" id="1744037at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000835">
        <property type="entry name" value="HTH_MarR-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12802">
        <property type="entry name" value="MarR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00598">
        <property type="entry name" value="HTHMARR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00347">
        <property type="entry name" value="HTH_MARR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50995">
        <property type="entry name" value="HTH_MARR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH marR-type" evidence="1">
        <location>
            <begin position="16"/>
            <end position="149"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF08679.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="183" mass="20115" checksum="F8B99033D332FE2A" modified="2006-05-30" version="1">MAQETSGTLQAPGRLVDFINYRLYHLSRVAMAASGLHLRAAAGVSRREWRMLAFLGEQPGTRLTELAASAGLDKVLASRAVHGLIDKGLVRRDSREGDRRAAAFRLTEAGESVYAAAFARARDFNRELAGCLNEDEAEMFSRCLDKLHAQAMTMLLAAKPLADRSDAADAPAFEDPLNVWRKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LMG6</accession>
    <name>Q1LMG6_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Catechol 1,2-dioxygenase</fullName>
            <ecNumber evidence="5">1.13.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">catA</name>
        <name evidence="11" type="ordered locus">Rmet_1781</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>catechol + O2 = cis,cis-muconate + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:23852"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:18135"/>
            <dbReference type="ChEBI" id="CHEBI:32379"/>
            <dbReference type="EC" id="1.13.11.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Fe(3+)</name>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Aromatic compound metabolism; beta-ketoadipate pathway; 5-oxo-4,5-dihydro-2-furylacetate from catechol: step 1/3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the intradiol ring-cleavage dioxygenase family.</text>
    </comment>
    <dbReference type="EC" id="1.13.11.1" evidence="5"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08660.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516513.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1781"/>
    <dbReference type="EnsemblBacteria" id="ABF08660">
        <property type="protein sequence ID" value="ABF08660"/>
        <property type="gene ID" value="Rmet_1781"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1781"/>
    <dbReference type="eggNOG" id="COG3485">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046727_0_0_4"/>
    <dbReference type="OMA" id="VWHANAL"/>
    <dbReference type="OrthoDB" id="1456634at2"/>
    <dbReference type="UniPathway" id="UPA00157">
        <property type="reaction ID" value="UER00258"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018576">
        <property type="term" value="F:catechol 1,2-dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008199">
        <property type="term" value="F:ferric iron binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042952">
        <property type="term" value="P:beta-ketoadipate pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019614">
        <property type="term" value="P:catechol-containing compound catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03460">
        <property type="entry name" value="1_2-CTD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.130.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007535">
        <property type="entry name" value="Catechol_dOase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012801">
        <property type="entry name" value="Cchol_dOase_prob"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000627">
        <property type="entry name" value="Intradiol_dOase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015889">
        <property type="entry name" value="Intradiol_dOase_core"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00775">
        <property type="entry name" value="Dioxygenase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04444">
        <property type="entry name" value="Dioxygenase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49482">
        <property type="entry name" value="SSF49482"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02439">
        <property type="entry name" value="catechol_proteo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00083">
        <property type="entry name" value="INTRADIOL_DIOXYGENAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 11" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="9" id="KW-0408">Iron</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="INTRADIOL_DIOXYGENAS" evidence="10">
        <location>
            <begin position="130"/>
            <end position="158"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001312"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001965"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004957"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00013118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS00083"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ABF08660.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="305" mass="33264" checksum="B9866D22BF6585B5" modified="2006-05-30" version="1">MDKIQIDTVLNRIEEATGQHANPRIAAIVHRMVRDLFHMIEDLDIQPEEFWSGLNFLAEAGRSGELGLIAPGLGFDHFLDLRMDEAESQAGIAGGTPRTIEGPLYVAGAPVAQREARLDDGTGEGEVLVMEGQVKDGAGQPLANAVVEVWHANALGNYSYFDPTQSAFNLRRSIVTDGQGRYRFRSIMPVGYSVPPNGATDRLLKQLDRHGTRPAHIHFFVSAPGHRKLTTQINIDGDPYLWDDFAFATREGLVPPITRVSDAAAMAALGVDKPFASIRFDFTLHADQADAPQAEVARQRASVAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="106" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LMU1</accession>
    <name>Q1LMU1_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">DNA-binding response regulator in two-component regulatory system with CreC</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">creB</name>
        <name evidence="6" type="ordered locus">Rmet_1652</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08535.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516380.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1652"/>
    <dbReference type="EnsemblBacteria" id="ABF08535">
        <property type="protein sequence ID" value="ABF08535"/>
        <property type="gene ID" value="Rmet_1652"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1652"/>
    <dbReference type="eggNOG" id="COG0745">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_30_4_4"/>
    <dbReference type="OMA" id="KRLGEPW"/>
    <dbReference type="OrthoDB" id="817710at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00383">
        <property type="entry name" value="trans_reg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001867">
        <property type="entry name" value="OmpR/PhoB-type_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016032">
        <property type="entry name" value="Sig_transdc_resp-reg_C-effctor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039420">
        <property type="entry name" value="WalR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48111">
        <property type="entry name" value="PTHR48111"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00486">
        <property type="entry name" value="Trans_reg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00862">
        <property type="entry name" value="Trans_reg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46894">
        <property type="entry name" value="SSF46894"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51755">
        <property type="entry name" value="OMPR_PHOB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 3 6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Response regulatory" evidence="4">
        <location>
            <begin position="5"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="domain" description="OmpR/PhoB-type" evidence="5">
        <location>
            <begin position="131"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="OmpR/PhoB-type" evidence="3">
        <location>
            <begin position="131"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="2">
        <location>
            <position position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABF08535.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="232" mass="26189" checksum="1D64445CD19BBE45" modified="2006-05-30" version="1">MKQPAVLVVEDESAIADTILYALRTEGMQVETCTLGSEALDRVRSRRPDLVILDVGLPDISGFEVCRSMRTFSDVPVIFLTARHEEIDRIVGLEIGADDYVVKPFSPRELAARVRVILRRAKGASDSPHPSPMAQEEADFVHDTDGARLAYRGRWLDLTRYEYRLLALLVQHPGRIFARSQLMDLVWHDSLDTVDRTVDTHVKTLRAKLREIAPAGDPIRTHRGMGYSLESR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="62" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LN38</accession>
    <name>Q1LN38_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Lipoprotein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_1555</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08438.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516292.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1555"/>
    <dbReference type="EnsemblBacteria" id="ABF08438">
        <property type="protein sequence ID" value="ABF08438"/>
        <property type="gene ID" value="Rmet_1555"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1555"/>
    <dbReference type="eggNOG" id="COG5314">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2168795_0_0_4"/>
    <dbReference type="OMA" id="DGAFCAQ"/>
    <dbReference type="OrthoDB" id="1918805at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193356" evidence="2">
        <location>
            <begin position="20"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="75"/>
            <end position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF08438.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="110" mass="11542" checksum="3675435EA846D9F0" modified="2006-05-30" version="1">MRCVLVLCAVLLAACGKQPANNLADALAADPVRLKALRAQCAAHPQQVMQATGEDACRAAAEAFRRRFFSGEAGPDEYQTAADLPPIPPSFDEPADGVAPAVPAQREDTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="60" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LNC7</accession>
    <name>Q1LNC7_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_1466</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08349.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516214.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1466"/>
    <dbReference type="EnsemblBacteria" id="ABF08349">
        <property type="protein sequence ID" value="ABF08349"/>
        <property type="gene ID" value="Rmet_1466"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1466"/>
    <dbReference type="eggNOG" id="ENOG5032BAX">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_183779_0_0_4"/>
    <dbReference type="OMA" id="ECRCGEF"/>
    <dbReference type="OrthoDB" id="2020445at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF08349.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="91" mass="10357" checksum="1A3B197BC62562AD" modified="2006-05-30" version="1">MARSPHPKKEVEEALRHAEGQGWRVEVGGSHAWGRIYCPYNDEECRCGEFCITSVWSTPKNPGNHARALRRVVDNCTTHRKQHEADDGSKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LND8</accession>
    <name>Q1LND8_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative protease, membrane anchored</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">ybbK</name>
        <name evidence="3" type="ordered locus">Rmet_1455</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the band 7/mec-2 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08338.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516204.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1455"/>
    <dbReference type="EnsemblBacteria" id="ABF08338">
        <property type="protein sequence ID" value="ABF08338"/>
        <property type="gene ID" value="Rmet_1455"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1455"/>
    <dbReference type="eggNOG" id="COG0330">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024949_2_2_4"/>
    <dbReference type="OMA" id="TQIRAEM"/>
    <dbReference type="OrthoDB" id="1213601at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008233">
        <property type="term" value="F:peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.479.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001107">
        <property type="entry name" value="Band_7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036013">
        <property type="entry name" value="Band_7/SPFH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032435">
        <property type="entry name" value="Band_7_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001972">
        <property type="entry name" value="Stomatin_HflK_HflKC_fam"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01145">
        <property type="entry name" value="Band_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16200">
        <property type="entry name" value="Band_7_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00721">
        <property type="entry name" value="STOMATIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00244">
        <property type="entry name" value="PHB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117892">
        <property type="entry name" value="SSF117892"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PHB" evidence="2">
        <location>
            <begin position="23"/>
            <end position="181"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008164"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF08338.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="312" mass="33838" checksum="A3781B726EC2E01C" modified="2006-05-30" version="1">MLAYQLGLFPLILLIAAIVLIAKSVKIVPQQHAWVLERLGRYHATLTPGLTVVVPFIDRVAYKHILKEIPLDVPSQVCITKDNTQLQVDGVLYFQVTDPMKASYGSSNFVVAITQLSQTTLRSVIGKLELDKTFEEREFINHSVVNALDEAAANWGVKVLRYEIKDLTPPKEILHAMQAQITAEREKRALIAASEGKRQEQINLASGAREAAIQKSEGERQAAINKAQGEAAAILAVAEANAQAIEKIGHAIRGEGGSEAVNLKVAEEYVAAFGNLAKQGNTLIVPGNLGEMSSMIASALQIVKGQKMEKAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LNJ7</accession>
    <name>Q1LNJ7_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">[Ribosomal protein S18]-alanine N-acetyltransferase</fullName>
            <ecNumber evidence="5">2.3.1.266</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5 7" type="primary">rimI</name>
        <name evidence="7" type="ordered locus">Rmet_1396</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Acetylates the N-terminal alanine of ribosomal protein S18.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>acetyl-CoA + N-terminal L-alanyl-[ribosomal protein bS18] = CoA + H(+) + N-terminal N(alpha)-acetyl-L-alanyl-[ribosomal protein bS18]</text>
            <dbReference type="Rhea" id="RHEA:43756"/>
            <dbReference type="Rhea" id="RHEA-COMP:10676"/>
            <dbReference type="Rhea" id="RHEA-COMP:10677"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:64718"/>
            <dbReference type="ChEBI" id="CHEBI:83683"/>
            <dbReference type="EC" id="2.3.1.266"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the acetyltransferase family. RimI subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.266" evidence="5"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08279.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516153.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1396"/>
    <dbReference type="EnsemblBacteria" id="ABF08279">
        <property type="protein sequence ID" value="ABF08279"/>
        <property type="gene ID" value="Rmet_1396"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1396"/>
    <dbReference type="eggNOG" id="COG0456">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013985_23_2_4"/>
    <dbReference type="OMA" id="GERYLNY"/>
    <dbReference type="OrthoDB" id="1826274at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008999">
        <property type="term" value="F:ribosomal protein S5-alanine N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017189">
        <property type="term" value="P:N-terminal peptidyl-alanine acetylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02210">
        <property type="entry name" value="RimI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006464">
        <property type="entry name" value="AcTrfase_RimI/Ard1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043690">
        <property type="entry name" value="RimI"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00583">
        <property type="entry name" value="Acetyltransf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="SSF55729"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01575">
        <property type="entry name" value="rimI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="2 5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5 7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="6">
        <location>
            <begin position="27"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="5">
        <location>
            <position position="129"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="5">
        <location>
            <position position="141"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="134"/>
        </location>
        <ligand>
            <name>acetyl-CoA</name>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005395"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABF08279.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="188" mass="20470" checksum="AC04BD08F5EF13B6" modified="2006-05-30" version="1">MSVAIAPVPDQPIWPATPTMPALPAGWALDRMSALDVEAVAEIEQRAFSHPWTRGNFENSLKAGHLGLTLRDPSAALIGYVMLMPVVDEMHLLNITIDPVFQRHGYGRLLLGIALATTHPHQLHTMLLEVRPSNTGALALYLDAGFVEIGRRKGYYPATALGREDALVLRRDWSADPSAALSTEEGTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="69" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LNL9</accession>
    <name>Q1LNL9_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative signalling protein EAL</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">eal</name>
        <name evidence="2" type="ordered locus">Rmet_1374</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08257.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516135.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1374"/>
    <dbReference type="EnsemblBacteria" id="ABF08257">
        <property type="protein sequence ID" value="ABF08257"/>
        <property type="gene ID" value="Rmet_1374"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1374"/>
    <dbReference type="eggNOG" id="COG2200">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_70_12_4"/>
    <dbReference type="OMA" id="VALDRMS"/>
    <dbReference type="OrthoDB" id="1635706at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.450">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001633">
        <property type="entry name" value="EAL_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035919">
        <property type="entry name" value="EAL_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00563">
        <property type="entry name" value="EAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF141868">
        <property type="entry name" value="SSF141868"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="EAL" evidence="1">
        <location>
            <begin position="39"/>
            <end position="252"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF08257.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="275" mass="30058" checksum="CCA6BBC40E16A92A" modified="2006-05-30" version="1">MTIDSALERYLQTLPREPVPGRELWRDVNGQVQGQYFQCSLTSAFEPLVTLADRGVVAHEGSIRTYAEDGVGLAAWKLFAMAADDTSLVSLDRLTRLVHAINYFAADGEHKLVLNVHNRLLAAVADDHGAAFRRALESLGLPIGRFVIQVPASANDDLPLLLHVVGNYRRNGFAVSLQASDPAEAGALMAHAMPDWLKLDMRRTWTDRQLSGLRASAEGAGVTLIGRRLQDDEAVERLQNAGIVLGQGRVIADEPVPARGLHVRAVPEQHKLERA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="67" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LNQ0</accession>
    <name>Q1LNQ0_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Conjugal transfer TrbD transmembrane protein (Protein belongs to CMGI-2)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">trbD</name>
        <name evidence="2" type="ordered locus">Rmet_1343</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08226.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_003138984.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1343"/>
    <dbReference type="EnsemblBacteria" id="ABF08226">
        <property type="protein sequence ID" value="ABF08226"/>
        <property type="gene ID" value="Rmet_1343"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1343"/>
    <dbReference type="eggNOG" id="COG5268">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_189159_0_0_4"/>
    <dbReference type="OMA" id="IWAVGHF"/>
    <dbReference type="OrthoDB" id="2047516at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016704">
        <property type="entry name" value="Conjugal_tfr_TrbD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007792">
        <property type="entry name" value="T4SS_VirB3/TrbD/AvhB"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05101">
        <property type="entry name" value="VirB3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017854">
        <property type="entry name" value="T4SS_TrbD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="49"/>
            <end position="68"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF08226.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="90" mass="9543" checksum="5890B9346CA761ED" modified="2006-05-30" version="1">MSGPDTFAAGFEVPLHRSLTEPILLGGAPRTVAIANGTLAAAVGLGLQLWIPGVVLWIVGHSLAVWGARVDPQFMAVFARHIKHRPLLDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LNT5</accession>
    <name>Q1LNT5_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">2-hydroxymuconic semialdehyde hydrolase (HMSH)-alpha/beta hydrolase superfamily (Belongs to CMGI-2)</fullName>
            <ecNumber evidence="2">3.4.11.5</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_1306</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EC" id="3.4.11.5" evidence="2"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08191.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516071.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1306"/>
    <dbReference type="ESTHER" id="burvg-a4jw31">
        <property type="family name" value="Carbon-carbon_bond_hydrolase"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF08191">
        <property type="protein sequence ID" value="ABF08191"/>
        <property type="gene ID" value="Rmet_1306"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1306"/>
    <dbReference type="eggNOG" id="COG2267">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020336_13_2_4"/>
    <dbReference type="OMA" id="HVPQIEC"/>
    <dbReference type="OrthoDB" id="1119700at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004177">
        <property type="term" value="F:aminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000639">
        <property type="entry name" value="Epox_hydrolase-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00561">
        <property type="entry name" value="Abhydrolase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00111">
        <property type="entry name" value="ABHYDROLASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00412">
        <property type="entry name" value="EPOXHYDRLASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="SSF53474"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0031">Aminopeptidase</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AB hydrolase-1" evidence="1">
        <location>
            <begin position="28"/>
            <end position="259"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF08191.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="276" mass="30788" checksum="F19547CB5EE8ED0D" modified="2006-05-30" version="1">MSDSLEIANTVTANGIKTNYHDRGQGVPVLLIHGSGPGVTAWANWRLTIPELAKQFRVIAPDMVGFGYTERPVGIRYDMDTWVGHALGLLDALGIDKAHVVGNSFGGALALALAIRAPERVRRLVLMGSVGISFPITEGLDKVWGYVPSIDNMRELLDVFAFDRNLVNDDLARLRYEASIRPNFQEAFSSMFPAPRQRWVDAMASREEDVGSLPHETLIIHGREDQVIPLSNSYTLLRLIPRAQLHVFGRCGHWTQIEHGERFNRLVGDFFSEEAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LNU8</accession>
    <name>Q1LNU8_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">HoxM protein involved in processing of HyaA and HyaB proteins Hydrolase Nickel Metalloprotease (Belongs to CMGI-2)Protease Absolutely required for hydrogenase activity</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">hoxM</name>
        <name evidence="4" type="ordered locus">Rmet_1293</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase A31 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08178.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516060.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1293"/>
    <dbReference type="MEROPS" id="A31.002"/>
    <dbReference type="EnsemblBacteria" id="ABF08178">
        <property type="protein sequence ID" value="ABF08178"/>
        <property type="gene ID" value="Rmet_1293"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1293"/>
    <dbReference type="eggNOG" id="COG0680">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_099037_0_1_4"/>
    <dbReference type="OMA" id="MGAKKMS"/>
    <dbReference type="OrthoDB" id="1858718at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008047">
        <property type="term" value="F:enzyme activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008237">
        <property type="term" value="F:metallopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016485">
        <property type="term" value="P:protein processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1450">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004419">
        <property type="entry name" value="Pept_A31_hyd_express"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023430">
        <property type="entry name" value="Pept_HybD-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000671">
        <property type="entry name" value="Peptidase_A31"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30302">
        <property type="entry name" value="PTHR30302"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01750">
        <property type="entry name" value="HycI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00446">
        <property type="entry name" value="HYDRGNUPTAKE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53163">
        <property type="entry name" value="SSF53163"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00140">
        <property type="entry name" value="hupD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00072">
        <property type="entry name" value="hydrog_prot"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="2" id="KW-0533">Nickel</keyword>
    <keyword evidence="4" id="KW-0645">Protease</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022596"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF08178.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="221" mass="24344" checksum="721E30D46B48A946" modified="2006-05-30" version="1">MRTTPTESTCHPGGDTLPARIVVLGIGNLLWADEGFGVRCIEALQRRWEFADHVQLIDGGTQGLYLIQHVQEADRLLIFDAIDYGLAPGTLKLVRDDDVPRFMGAKKMSLHQTGFQEVLSLATLTGKYPQQVLLIGCQPQELEDYGGSLRPVVKAAMEDALALGVAELERWGARPVERRTPLAPREAVTLDELALTAYEAQRPAAEEACRIGDDRLLVKDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LP14</accession>
    <name>Q1LP14_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Extracellular ligand-binding receptor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_1226</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08112.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516001.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1226"/>
    <dbReference type="EnsemblBacteria" id="ABF08112">
        <property type="protein sequence ID" value="ABF08112"/>
        <property type="gene ID" value="Rmet_1226"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1226"/>
    <dbReference type="eggNOG" id="COG0683">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027128_1_2_4"/>
    <dbReference type="OMA" id="NGFRMYV"/>
    <dbReference type="OrthoDB" id="538961at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028081">
        <property type="entry name" value="Leu-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028082">
        <property type="entry name" value="Peripla_BP_I"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13458">
        <property type="entry name" value="Peripla_BP_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53822">
        <property type="entry name" value="SSF53822"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0675">Receptor</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193712" evidence="1">
        <location>
            <begin position="29"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="domain" description="Peripla_BP_6" evidence="2">
        <location>
            <begin position="37"/>
            <end position="375"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF08112.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="397" mass="42408" checksum="D371776C029DA234" modified="2006-05-30" version="1">MHRNASRRLLPACLAVATLLATGSVALAQSAPATSSKIKVGFMLPYTGTYAALGGAIENGFRMYVQEQGGKLGGREVEYFKVDDESDPAKAPENATKLIKRDQVDVVVGTVHSGVQMGIVKVAKENNTLLIIPNAGVDEATGPLCGSNIFRSSFSNWQPGYAMGHVLADRGLKKVVTLTWKYAAGEQSVKGFKEAFEAKGGKVVKELSLPFPNVEFQALITEVASIKPDAVFVFFAGGGAVKFVKDWQAAGLKDKIPLYASGFLTDGTLEAQGVAAQGLETTLHYADGLTNPRDKSFRLDYAKLYKLQPDVYAVQGYDAAQLLAAGANAVKGDMSRKPEVIKAMEAAKVDSPRGAFTLSKAHNPVQDFYLRKVDGRDNKVSSVAVKALADPARGCRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LP54</accession>
    <name>Q1LP54_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative hydroxydechloroatrazine ethylaminohydrolase, AtzB (Hydroxyatrazine hydrolase)</fullName>
            <ecNumber evidence="3">3.5.99.3</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">atzB</name>
        <name evidence="3" type="ordered locus">Rmet_1186</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EC" id="3.5.99.3" evidence="3"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08072.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1186"/>
    <dbReference type="EnsemblBacteria" id="ABF08072">
        <property type="protein sequence ID" value="ABF08072"/>
        <property type="gene ID" value="Rmet_1186"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1186"/>
    <dbReference type="eggNOG" id="COG0402">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012358_2_3_4"/>
    <dbReference type="OMA" id="TWLAHGI"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016810">
        <property type="term" value="F:hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006680">
        <property type="entry name" value="Amidohydro-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011059">
        <property type="entry name" value="Metal-dep_hydrolase_composite"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01979">
        <property type="entry name" value="Amidohydro_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51338">
        <property type="entry name" value="SSF51338"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="SSF51556"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Amidohydro-rel" evidence="2">
        <location>
            <begin position="68"/>
            <end position="433"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF08072.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="467" mass="50359" checksum="3FC322A028512AED" modified="2006-05-30" version="1">MHITMTLILQHADVLVTMDAQRREIADGALVANGPAVEWVGPTAELPEQYRRLVDEGRAEVVDMRGHVVMPGLVNTHHHMYQSLTRAVPAAQDAELFGWLTNLYMLWSHLTPEMIRVSTQTAMAELMLSGCTTTSDHLYLYPNGSRLDDSIDAARQIGMRFHAARGSMSVGRSQGGLPPDVVVETEAAILRDSQRLVEQYHDASRHAMLRVVLAPCSPFSVSRDLMRESAKMARHYGVSLHTHLAENDNDIAYSREKFGMTPAQYAEDLGWVGHDVWHAHCVKLDEEGVALFARTGTGVAHCPCSNMRLASGIAPIRRMRDAGVPVGLGVGGSASNDGAHMLGEARQAMLLQRVGYGPAAMSAREALEIATLGGAKVLNRDDIGALAPGMSADIVAFDMSGVGYAGAGHDPVAALVFCTPGNVSMSVINGRVVIRDGHLLTADLPNVLSDHRRLARTLFERAQAAGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="99" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LP81</accession>
    <name>Q1LP81_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">(P)ppGpp synthetase I/GTP pyrophosphokinase</fullName>
            <ecNumber evidence="7">2.7.6.5</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">relA</name>
        <name evidence="7" type="ordered locus">Rmet_1159</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">In eubacteria ppGpp (guanosine 3'-diphosphate 5'-diphosphate) is a mediator of the stringent response that coordinates a variety of cellular activities in response to changes in nutritional abundance.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the relA/spoT family.</text>
    </comment>
    <dbReference type="EC" id="2.7.6.5" evidence="7"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08045.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515943.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1159"/>
    <dbReference type="EnsemblBacteria" id="ABF08045">
        <property type="protein sequence ID" value="ABF08045"/>
        <property type="gene ID" value="Rmet_1159"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1159"/>
    <dbReference type="eggNOG" id="COG0317">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012300_3_0_4"/>
    <dbReference type="OMA" id="TEIGHNC"/>
    <dbReference type="OrthoDB" id="204079at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008728">
        <property type="term" value="F:GTP diphosphokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016779">
        <property type="term" value="F:nucleotidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015969">
        <property type="term" value="P:guanosine tetraphosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05399">
        <property type="entry name" value="NT_Rel-Spo_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01668">
        <property type="entry name" value="TGS_RSH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.460.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045865">
        <property type="entry name" value="ACT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002912">
        <property type="entry name" value="ACT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012675">
        <property type="entry name" value="Beta-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043519">
        <property type="entry name" value="NT_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004811">
        <property type="entry name" value="RelA/Spo_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045600">
        <property type="entry name" value="RelA/SpoT_AH_RIS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007685">
        <property type="entry name" value="RelA_SpoT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004095">
        <property type="entry name" value="TGS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012676">
        <property type="entry name" value="TGS-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033655">
        <property type="entry name" value="TGS_RelA/SpoT"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13291">
        <property type="entry name" value="ACT_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19296">
        <property type="entry name" value="DUF5913"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04607">
        <property type="entry name" value="RelA_SpoT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02824">
        <property type="entry name" value="TGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00954">
        <property type="entry name" value="RelA_SpoT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55021">
        <property type="entry name" value="SSF55021"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81271">
        <property type="entry name" value="SSF81271"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81301">
        <property type="entry name" value="SSF81301"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00691">
        <property type="entry name" value="spoT_relA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51671">
        <property type="entry name" value="ACT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51880">
        <property type="entry name" value="TGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="2" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="1" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="TGS" evidence="6">
        <location>
            <begin position="411"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="domain" description="ACT" evidence="5">
        <location>
            <begin position="671"/>
            <end position="742"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABF08045.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="742" mass="82898" checksum="AB115DFA86DF5D17" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="59" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LPP3</accession>
    <name>Q1LPP3_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_0997</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07883.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515798.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0997"/>
    <dbReference type="EnsemblBacteria" id="ABF07883">
        <property type="protein sequence ID" value="ABF07883"/>
        <property type="gene ID" value="Rmet_0997"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0997"/>
    <dbReference type="HOGENOM" id="CLU_1746574_0_0_4"/>
    <dbReference type="OrthoDB" id="1774441at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="116"/>
            <end position="141"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF07883.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="141" mass="14846" checksum="C67B67946DF726AC" modified="2006-05-30" version="1">MTHGKSPSLHRRVVAATFAALAVTAVSGCASESKSFAPPRGSITDDRAIGDILVKFTPPDVTTSEDAGKLLAAVEGPVRYVVKRPMSGGVWLVTAITKSPDITADQAVATLQAAPRVESATPDRMLKPHRSMPVGRDMPQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LPT1</accession>
    <name>Q1LPT1_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Urea amidohydrolase (Urease) accessory protein UreJ</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">ureJ</name>
        <name evidence="3" type="ordered locus">Rmet_0959</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07845.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515767.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0959"/>
    <dbReference type="EnsemblBacteria" id="ABF07845">
        <property type="protein sequence ID" value="ABF07845"/>
        <property type="gene ID" value="Rmet_0959"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0959"/>
    <dbReference type="eggNOG" id="COG2370">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_088877_0_1_4"/>
    <dbReference type="OMA" id="PFGMGES"/>
    <dbReference type="OrthoDB" id="1908275at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007038">
        <property type="entry name" value="HupE_UreJ"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04955">
        <property type="entry name" value="HupE_UreJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016919">
        <property type="entry name" value="HupE_UreJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193686" evidence="2">
        <location>
            <begin position="25"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="79"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="105"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="130"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="161"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="192"/>
            <end position="210"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF07845.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="211" mass="21184" checksum="044E7DB39FEC0B79" modified="2006-05-30" version="1">MHRMLRSRLTAGVAMSLASVSAFAHPGHDAATVGASLWAGLAHPFTGADHLLAMAAVGVWSALASSPRSAFGQTSKMDLMRLPLAFVAMMLVGAALGLAGVTLPAVEPMIAVSLLVIGLLVAARARLSAWAGMAIVGGFALFHGYAHGAELPATAAALPSVLAYVGGFAVATMTLHVLGIGAGAFLRRHAGWLARVAGAGVALYGVGLLVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LPW4</accession>
    <name>Q1LPW4_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 10">Protein-export membrane protein SecG</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">secG</name>
        <name evidence="11" type="ordered locus">Rmet_0926</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Involved in protein export. Participates in an early event of protein translocation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Cell membrane</location>
            <topology evidence="10">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the SecG family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07812.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515747.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0926"/>
    <dbReference type="EnsemblBacteria" id="ABF07812">
        <property type="protein sequence ID" value="ABF07812"/>
        <property type="gene ID" value="Rmet_0926"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0926"/>
    <dbReference type="eggNOG" id="COG1314">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_094156_2_0_4"/>
    <dbReference type="OMA" id="VIHLMIV"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015450">
        <property type="term" value="F:protein-transporting ATPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009306">
        <property type="term" value="P:protein secretion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004692">
        <property type="entry name" value="SecG"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34182">
        <property type="entry name" value="PTHR34182"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03840">
        <property type="entry name" value="SecG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01651">
        <property type="entry name" value="SECGEXPORT"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00810">
        <property type="entry name" value="secG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 10" id="KW-0653">Protein transport</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 10" id="KW-0811">Translocation</keyword>
    <keyword evidence="5 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4 10" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="54"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008445"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00017876"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU365087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ABF07812.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="122" mass="11571" checksum="404A93A92321ABE8" modified="2006-05-30" version="1">MAIFKTLLVIVQVLSALGVIGLVLLQHGKGADVGAAFGSGASGSLFGATGSANFLSRTTAVLAALFFCCTLGLTLLGNYKPSASLGVMGAAQQSAPAAAAPASAPAAAAASASAPAAPAVPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="60" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LPY1</accession>
    <name>Q1LPY1_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_0909</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07795.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515735.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0909"/>
    <dbReference type="EnsemblBacteria" id="ABF07795">
        <property type="protein sequence ID" value="ABF07795"/>
        <property type="gene ID" value="Rmet_0909"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0909"/>
    <dbReference type="eggNOG" id="ENOG50333E6">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_131372_0_0_4"/>
    <dbReference type="OMA" id="AGIYQWQ"/>
    <dbReference type="OrthoDB" id="2054212at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022064">
        <property type="entry name" value="DUF3619"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12279">
        <property type="entry name" value="DUF3619"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="87"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF07795.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="145" mass="16226" checksum="08E128FC650C8BC8" modified="2006-05-30" version="1">MSRNERDIQERRFAHEIRLALDASADALSPDVAERLAAARRMALAAKKAEVRVHVPQLALPGGHHGYAFEEEDDTPLHRAGAWLRKLALVWTLVALGAGLIGIYHWQQQKRVEELADVDTAMLLDDLPPTAYADQGFHVYLKRGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LQ14</accession>
    <name>Q1LQ14_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Aminopeptidase N</fullName>
            <ecNumber evidence="4">3.4.11.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">pepN</name>
        <name evidence="15" type="ordered locus">Rmet_0876</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Release of an N-terminal amino acid, Xaa-|-Yaa- from a peptide, amide or arylamide. Xaa is preferably Ala, but may be most amino acids including Pro (slow action). When a terminal hydrophobic residue is followed by a prolyl residue, the two may be released as an intact Xaa-Pro dipeptide.</text>
            <dbReference type="EC" id="3.4.11.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the peptidase M1 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.11.2" evidence="4"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07762.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515702.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0876"/>
    <dbReference type="MEROPS" id="M01.005"/>
    <dbReference type="EnsemblBacteria" id="ABF07762">
        <property type="protein sequence ID" value="ABF07762"/>
        <property type="gene ID" value="Rmet_0876"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0876"/>
    <dbReference type="eggNOG" id="COG0308">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007993_2_0_4"/>
    <dbReference type="OMA" id="FKRWYSQ"/>
    <dbReference type="OrthoDB" id="771837at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004177">
        <property type="term" value="F:aminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008237">
        <property type="term" value="F:metallopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.390.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.50.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1730">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1840">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045357">
        <property type="entry name" value="Aminopeptidase_N-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042097">
        <property type="entry name" value="Aminopeptidase_N-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038438">
        <property type="entry name" value="PepN_Ig-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001930">
        <property type="entry name" value="Peptidase_M1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014782">
        <property type="entry name" value="Peptidase_M1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012779">
        <property type="entry name" value="Peptidase_M1_pepN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024601">
        <property type="entry name" value="Peptidase_M1_pepN_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037144">
        <property type="entry name" value="Peptidase_M1_pepN_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035414">
        <property type="entry name" value="Peptidase_M1_pepN_Ig-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027268">
        <property type="entry name" value="Peptidase_M4/M1_CTD_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46322">
        <property type="entry name" value="PTHR46322"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11940">
        <property type="entry name" value="DUF3458"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17432">
        <property type="entry name" value="DUF3458_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01433">
        <property type="entry name" value="Peptidase_M1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17900">
        <property type="entry name" value="Peptidase_M1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00756">
        <property type="entry name" value="ALADIPTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63737">
        <property type="entry name" value="SSF63737"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02414">
        <property type="entry name" value="pepN_proteo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="15" id="KW-0031">Aminopeptidase</keyword>
    <keyword evidence="8 15" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="6" id="KW-0645">Protease</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Peptidase_M1_N" evidence="14">
        <location>
            <begin position="50"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase_M1" evidence="11">
        <location>
            <begin position="228"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="domain" description="DUF3458" evidence="12">
        <location>
            <begin position="450"/>
            <end position="562"/>
        </location>
    </feature>
    <feature type="domain" description="DUF3458_C" evidence="13">
        <location>
            <begin position="566"/>
            <end position="895"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012564"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00015611"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF01433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF11940"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF17432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF17900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="ABF07762.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="897" mass="99936" checksum="FE517DF445E8EA05" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="95" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LQ22</accession>
    <name>Q1LQ22_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Regulatory protein GntR, HTH:Aminotransferase, class I and II</fullName>
            <ecNumber evidence="3">2.6.1.39</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">gntR</name>
        <name evidence="3" type="ordered locus">Rmet_0868</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">In the C-terminal section; belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.6.1.39" evidence="3"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07754.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0868"/>
    <dbReference type="EnsemblBacteria" id="ABF07754">
        <property type="protein sequence ID" value="ABF07754"/>
        <property type="gene ID" value="Rmet_0868"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0868"/>
    <dbReference type="eggNOG" id="COG1167">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017584_0_0_4"/>
    <dbReference type="OMA" id="SPTYWGA"/>
    <dbReference type="OrthoDB" id="1024978at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047536">
        <property type="term" value="F:2-aminoadipate transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009058">
        <property type="term" value="P:biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07377">
        <property type="entry name" value="WHTH_GntR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000524">
        <property type="entry name" value="Tscrpt_reg_HTH_GntR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00392">
        <property type="entry name" value="GntR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00345">
        <property type="entry name" value="HTH_GNTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="SSF53383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50949">
        <property type="entry name" value="HTH_GNTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="HTH gntR-type" evidence="2">
        <location>
            <begin position="134"/>
            <end position="202"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF07754.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="606" mass="65633" checksum="85D9790609490412" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LQA9</accession>
    <name>Q1LQA9_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Ribosomal silencing factor RsfS</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">rsfS</name>
        <name evidence="7" type="ordered locus">Rmet_0781</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Functions as a ribosomal silencing factor. Interacts with ribosomal protein L14 (rplN), blocking formation of intersubunit bridge B8. Prevents association of the 30S and 50S ribosomal subunits and the formation of functional ribosomes, thus repressing translation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Interacts with ribosomal protein L14 (rplN).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the Iojap/RsfS family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07667.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515621.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0781"/>
    <dbReference type="EnsemblBacteria" id="ABF07667">
        <property type="protein sequence ID" value="ABF07667"/>
        <property type="gene ID" value="Rmet_0781"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0781"/>
    <dbReference type="eggNOG" id="COG0799">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_092688_0_0_4"/>
    <dbReference type="OMA" id="VAHIMQP"/>
    <dbReference type="OrthoDB" id="1990650at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016779">
        <property type="term" value="F:nucleotidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042256">
        <property type="term" value="P:mature ribosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008152">
        <property type="term" value="P:metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090071">
        <property type="term" value="P:negative regulation of ribosome biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017148">
        <property type="term" value="P:negative regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.460.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01477">
        <property type="entry name" value="Iojap_RsfS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004394">
        <property type="entry name" value="Iojap/RsfS/C7orf30"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043519">
        <property type="entry name" value="NT_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21043">
        <property type="entry name" value="PTHR21043"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81301">
        <property type="entry name" value="SSF81301"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00090">
        <property type="entry name" value="rsfS_iojap_ybeB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="2" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0678">Repressor</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0810">Translation regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="128"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="130"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="166"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="185"/>
            <end position="242"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABF07667.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="256" mass="27453" checksum="B93B373B0A3E4435" modified="2006-05-30" version="1">MKLNKKDTMDIRKLQRAIVDGLEDVKAQDIKVFDTTHLTELFDRVVIASGNSNRQTKALAASVRDTVKDAGGHIVAVEGLETGEWVLVDCGDAVVHILQPQLRLYYNLEEIWGDKPVRVKLAAGKGLAKASEPMDDEDAEPAPRVRRASNLRPALARLPEGMKEPSPPMGHEDTDPDAIATIRKPARKTSSTGTAAKAPARKTTTARKTTTGTAAKTPARKSATGTATKTTARKTTATKTAAAKTAAPRKRATRSA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-05-20" modified="2022-05-25" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LQK4</accession>
    <name>LOLA_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Outer-membrane lipoprotein carrier protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">lolA</name>
        <name type="ordered locus">Rmet_0686</name>
    </gene>
    <organism>
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Participates in the translocation of lipoproteins from the inner membrane to the outer membrane. Only forms a complex with a lipoprotein if the residue after the N-terminal Cys is not an aspartate (The Asp acts as a targeting signal to indicate that the lipoprotein should stay in the inner membrane).</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Periplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the LolA family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07572.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515539.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q1LQK4"/>
    <dbReference type="SMR" id="Q1LQK4"/>
    <dbReference type="STRING" id="266264.Rmet_0686"/>
    <dbReference type="EnsemblBacteria" id="ABF07572">
        <property type="protein sequence ID" value="ABF07572"/>
        <property type="gene ID" value="Rmet_0686"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0686"/>
    <dbReference type="eggNOG" id="COG2834">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_087560_0_1_4"/>
    <dbReference type="OMA" id="YDPFVEQ"/>
    <dbReference type="OrthoDB" id="1933827at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042597">
        <property type="term" value="C:periplasmic space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044874">
        <property type="term" value="P:lipoprotein localization to outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042953">
        <property type="term" value="P:lipoprotein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd16325">
        <property type="entry name" value="LolA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00240">
        <property type="entry name" value="LolA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029046">
        <property type="entry name" value="LolA/LolB/LppX"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004564">
        <property type="entry name" value="OM_lipoprot_carrier_LolA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018323">
        <property type="entry name" value="OM_lipoprot_carrier_LolA_Pbac"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35869">
        <property type="entry name" value="PTHR35869"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03548">
        <property type="entry name" value="LolA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89392">
        <property type="entry name" value="SSF89392"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00547">
        <property type="entry name" value="lolA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0143">Chaperone</keyword>
    <keyword id="KW-0574">Periplasm</keyword>
    <keyword id="KW-0653">Protein transport</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0732">Signal</keyword>
    <keyword id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5000118536" description="Outer-membrane lipoprotein carrier protein">
        <location>
            <begin position="28"/>
            <end position="214"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00240"/>
        </source>
    </evidence>
    <sequence length="214" mass="23249" checksum="D538A20A16FD1B45" modified="2006-05-30" version="1" precursor="true">MRYRILAPLVSLGAALALLAAAPVAQAAATDQLQSFVTSVKTARGEFTQQQMKGKGADLKVASTSSGTFVFSRPGKFAWRYTKPYEQLLQADGQVLYIYDKDLNQVTERKLDGALGSSPAAILFGSNDLSKNFDVKNGATRDGVEWLELTPKEKDTQFERIGIGFKGGNLEAMELRDAFGNTTILTFSAIQKNPSLPADAFRFTVPKGADVMKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LQM5</accession>
    <name>Q1LQM5_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">LemA like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">lemA</name>
        <name evidence="7" type="ordered locus">Rmet_0665</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the LemA family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07551.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515522.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0665"/>
    <dbReference type="EnsemblBacteria" id="ABF07551">
        <property type="protein sequence ID" value="ABF07551"/>
        <property type="gene ID" value="Rmet_0665"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0665"/>
    <dbReference type="eggNOG" id="COG1704">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_056714_0_0_4"/>
    <dbReference type="OMA" id="ACGYNDF"/>
    <dbReference type="OrthoDB" id="1573349at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1440.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023353">
        <property type="entry name" value="LemA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007156">
        <property type="entry name" value="MamQ_LemA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34478">
        <property type="entry name" value="PTHR34478"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04011">
        <property type="entry name" value="LemA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140478">
        <property type="entry name" value="SSF140478"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193435" evidence="6">
        <location>
            <begin position="25"/>
            <end position="200"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008854"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABF07551.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="200" mass="22195" checksum="A112486D74316FCB" modified="2006-05-30" version="1">MRAATSTALFRWLAIAMLASLLSACGYNDFQAKDEAVKAAWGEVINQYQRRADLIPNLVATVKGYATHERETLEAVTKARAEATSIKVTPETLNDPEAFARFQQAQGQLTSALSRLLAVSENYPQLKADASFRDLQSQLEGTENRITVARQRYIAAVQDYNVLARSFPTNLTAMIFKYPVKPSFTVENEKSISTPPAVKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="70" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LQR5</accession>
    <name>Q1LQR5_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_0625</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07511.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515482.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0625"/>
    <dbReference type="EnsemblBacteria" id="ABF07511">
        <property type="protein sequence ID" value="ABF07511"/>
        <property type="gene ID" value="Rmet_0625"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0625"/>
    <dbReference type="eggNOG" id="COG3516">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_111033_1_0_4"/>
    <dbReference type="OMA" id="LMVMGDY"/>
    <dbReference type="OrthoDB" id="1278805at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008312">
        <property type="entry name" value="T6SS_TssB1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35850">
        <property type="entry name" value="PTHR35850"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05591">
        <property type="entry name" value="T6SS_VipA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF028301">
        <property type="entry name" value="UCP028301"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03358">
        <property type="entry name" value="VI_chp_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF07511.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="171" mass="19059" checksum="01204731EDA2F9AD" modified="2006-05-30" version="1">MAESFQNEVPKARVNIKLDLHTGGAQKKVELPLKLLVMGDYSNGQETRALAEREKVSINKNNFNSVLADFNPKARIAVESTLARDGSELPVDLDFKSMDDFKPEVVASRVPELRALMAARNLLRDLKSNLLDNAMFRRELEKILKDPGLSERLRADLQKISEAAPASQSAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LR58</accession>
    <name>Q1LR58_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_0482</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07368.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515353.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF07368">
        <property type="protein sequence ID" value="ABF07368"/>
        <property type="gene ID" value="Rmet_0482"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0482"/>
    <dbReference type="HOGENOM" id="CLU_1650724_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF07368.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="160" mass="18023" checksum="F163E293B9592CB0" modified="2006-05-30" version="1">MLYHLVMETSIWISLASLVISGSLSSAAFLHTRRDSLLKLRREALAHARASAVEWQCVLNDIEALKLGEVRSLLPMPSRAEFDAWLNAVHDTFKGSTERAQAIAENVSANFHKLTRHEAILVIRACEDGTPVVSAAREELRRKMEDFAKRLEREASRRVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="68" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LRB9</accession>
    <name>Q1LRB9_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Ysc84 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_0421</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07307.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515296.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF07307">
        <property type="protein sequence ID" value="ABF07307"/>
        <property type="gene ID" value="Rmet_0421"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0421"/>
    <dbReference type="eggNOG" id="COG2930">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_100983_0_0_4"/>
    <dbReference type="OMA" id="MTPEAYN"/>
    <dbReference type="OrthoDB" id="1808930at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007461">
        <property type="entry name" value="Ysc84_actin-binding"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04366">
        <property type="entry name" value="Ysc84"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193658" description="Ysc84 domain-containing protein" evidence="1">
        <location>
            <begin position="23"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="domain" description="Ysc84" evidence="2">
        <location>
            <begin position="108"/>
            <end position="190"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF04366"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF07307.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="194" mass="20003" checksum="4934FC0B5AFD3007" modified="2006-05-30" version="1">MNRRNFVTRMAGSGLLVATAMAAGCTTTGSSDSKDAAARRQEIDAGADGALNRLFTSVNGSRELADKAQGILVFPRVLAGGLVIGGEYGDGVLRSKGANVGYYRTLAASFGLTAGGQSRSVIIMFMTPEAYNKFLQAKGWTAGVDASVALAKVGANGKVDTVTGQQPVIGFVQTNAGLMFDVSLDGSKISKLDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="65" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LRK8</accession>
    <name>Q1LRK8_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Lactoylglutathione lyase (Glyoxalase family) CMGI-7</fullName>
            <ecNumber evidence="2">4.4.1.5</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">arsI</name>
        <name evidence="2" type="ordered locus">Rmet_0332</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EC" id="4.4.1.5" evidence="2"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07218.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515213.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0332"/>
    <dbReference type="EnsemblBacteria" id="ABF07218">
        <property type="protein sequence ID" value="ABF07218"/>
        <property type="gene ID" value="Rmet_0332"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0332"/>
    <dbReference type="eggNOG" id="COG0346">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_125391_0_0_4"/>
    <dbReference type="OMA" id="CCYALQD"/>
    <dbReference type="OrthoDB" id="1984437at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004462">
        <property type="term" value="F:lactoylglutathione lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.180.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029068">
        <property type="entry name" value="Glyas_Bleomycin-R_OHBP_Dase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037523">
        <property type="entry name" value="VOC"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54593">
        <property type="entry name" value="SSF54593"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51819">
        <property type="entry name" value="VOC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0456">Lyase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="VOC" evidence="1">
        <location>
            <begin position="2"/>
            <end position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF07218.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="162" mass="17730" checksum="ABF059CCB705C251" modified="2006-05-30" version="1">MKRFHVHVSVADLPASIRYYSALFDAEPTVVRDDYAKWALDDPRVNFAISNRTEKLGVDHLGIQVETDAELAEMQGRLASADLPMQSQSETNCCYATSNKHWSVDPTGIAWESFHTLGNVPTYGESRDRSAEASACCVPVQLNATPKPSTKTACCTPESGCC</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LRR2</accession>
    <name>Q1LRR2_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Probable response regulator (CheY-like) and ANTAR domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_0278</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07164.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0278"/>
    <dbReference type="EnsemblBacteria" id="ABF07164">
        <property type="protein sequence ID" value="ABF07164"/>
        <property type="gene ID" value="Rmet_0278"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0278"/>
    <dbReference type="eggNOG" id="COG3707">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_65_1_4"/>
    <dbReference type="OMA" id="QKTSMDR"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005561">
        <property type="entry name" value="ANTAR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008327">
        <property type="entry name" value="Sig_transdc_resp-reg_antiterm"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03861">
        <property type="entry name" value="ANTAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036382">
        <property type="entry name" value="RR_antiterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01012">
        <property type="entry name" value="ANTAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50921">
        <property type="entry name" value="ANTAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ANTAR" evidence="2">
        <location>
            <begin position="154"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50921"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF07164.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="219" mass="24103" checksum="32B924123A47EFD4" modified="2006-05-30" version="1">MHRNQAPSMTRRHPPPSAVAPPATTSRSLRILLVRDPHEADPLNVETIRAGLAQAGFTEVQTVDADLRLPDTITASQPDLVIIASESAARDTIEHVCVSTQHAPRPIVLFTDNDDAQRIKAALSAGITAYIVDGLRAERVKTVLDVAYARFQLDQQLRAELDATKLKLAERKTVERAKGLLMQARGISEDEAFKRLRSMAMERGIRLVDAAQRVIDVMA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LS01</accession>
    <name>Q1LS01_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative type-4 fimbrial biogenesis pilV transmembrane protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_0189</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07075.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515095.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0189"/>
    <dbReference type="EnsemblBacteria" id="ABF07075">
        <property type="protein sequence ID" value="ABF07075"/>
        <property type="gene ID" value="Rmet_0189"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0189"/>
    <dbReference type="eggNOG" id="COG4967">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_103234_3_0_4"/>
    <dbReference type="OMA" id="RMRANRT"/>
    <dbReference type="OrthoDB" id="1683932at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012902">
        <property type="entry name" value="N_methyl_site"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07963">
        <property type="entry name" value="N_methyl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02532">
        <property type="entry name" value="IV_pilin_GFxxxE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF07075.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="180" mass="18723" checksum="A41C9C6E97133418" modified="2006-05-30" version="1">MQLKTTGNRHAAGFSLLEVLIALVITVIGLFGIAKMQAAAIANTQVARGQSLIALQLESLAGAMHGNKGFWAAGLVPATFSMTGATVTDTTGKLSTTAPDCKATTCTPTQLAAYDVQGWAAEMNAHFPTYSAAVNCVVLINKPVTCTVSANWNESNLAYNQTTATLAGQTAQSLTLYVQP</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-01-15" modified="2022-08-03" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LS11</accession>
    <name>CCA_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Multifunctional CCA protein</fullName>
        </recommendedName>
        <domain>
            <recommendedName>
                <fullName evidence="1">CCA-adding enzyme</fullName>
                <ecNumber evidence="1">2.7.7.72</ecNumber>
            </recommendedName>
            <alternativeName>
                <fullName evidence="1">CCA tRNA nucleotidyltransferase</fullName>
            </alternativeName>
            <alternativeName>
                <fullName evidence="1">tRNA CCA-pyrophosphorylase</fullName>
            </alternativeName>
            <alternativeName>
                <fullName evidence="1">tRNA adenylyl-/cytidylyl-transferase</fullName>
            </alternativeName>
            <alternativeName>
                <fullName evidence="1">tRNA nucleotidyltransferase</fullName>
            </alternativeName>
            <alternativeName>
                <fullName evidence="1">tRNA-NT</fullName>
            </alternativeName>
        </domain>
        <domain>
            <recommendedName>
                <fullName evidence="1">2'-nucleotidase</fullName>
                <ecNumber evidence="1">3.1.3.-</ecNumber>
            </recommendedName>
        </domain>
        <domain>
            <recommendedName>
                <fullName evidence="1">2',3'-cyclic phosphodiesterase</fullName>
                <ecNumber evidence="1">3.1.4.-</ecNumber>
            </recommendedName>
        </domain>
        <domain>
            <recommendedName>
                <fullName evidence="1">Phosphatase</fullName>
                <ecNumber evidence="1">3.1.3.-</ecNumber>
            </recommendedName>
        </domain>
    </protein>
    <gene>
        <name evidence="1" type="primary">cca</name>
        <name type="ordered locus">Rmet_0179</name>
    </gene>
    <organism>
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the addition and repair of the essential 3'-terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing inorganic pyrophosphate. Also shows phosphatase, 2'-nucleotidase and 2',3'-cyclic phosphodiesterase activities. These phosphohydrolase activities are probably involved in the repair of the tRNA 3'-CCA terminus degraded by intracellular RNases.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a tRNA precursor + ATP + 2 CTP = a tRNA with a 3' CCA end + 3 diphosphate</text>
            <dbReference type="Rhea" id="RHEA:14433"/>
            <dbReference type="Rhea" id="RHEA-COMP:10465"/>
            <dbReference type="Rhea" id="RHEA-COMP:10468"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
            <dbReference type="ChEBI" id="CHEBI:74896"/>
            <dbReference type="ChEBI" id="CHEBI:83071"/>
            <dbReference type="EC" id="2.7.7.72"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="1">Magnesium is required for nucleotidyltransferase activity.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Ni(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:49786"/>
        </cofactor>
        <text evidence="1">Nickel for phosphatase activity.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Monomer. Can also form homodimers and oligomers.</text>
    </comment>
    <comment type="domain">
        <text evidence="1">Comprises two domains: an N-terminal domain containing the nucleotidyltransferase activity and a C-terminal HD domain associated with both phosphodiesterase and phosphatase activities.</text>
    </comment>
    <comment type="miscellaneous">
        <text evidence="1">A single active site specifically recognizes both ATP and CTP and is responsible for their addition.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. Bacterial CCA-adding enzyme type 1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.72" evidence="1"/>
    <dbReference type="EC" id="3.1.3.-" evidence="1"/>
    <dbReference type="EC" id="3.1.4.-" evidence="1"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07065.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515088.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q1LS11"/>
    <dbReference type="SMR" id="Q1LS11"/>
    <dbReference type="STRING" id="266264.Rmet_0179"/>
    <dbReference type="EnsemblBacteria" id="ABF07065">
        <property type="protein sequence ID" value="ABF07065"/>
        <property type="gene ID" value="Rmet_0179"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0179"/>
    <dbReference type="eggNOG" id="COG0617">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015961_1_1_4"/>
    <dbReference type="OMA" id="GWTFHGH"/>
    <dbReference type="OrthoDB" id="2018439at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052929">
        <property type="term" value="F:ATP:3'-cytidine-cytidine-tRNA adenylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052928">
        <property type="term" value="F:CTP:3'-cytidine-tRNA cytidylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052927">
        <property type="term" value="F:CTP:tRNA cytidylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004112">
        <property type="term" value="F:cyclic-nucleotide phosphodiesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016791">
        <property type="term" value="F:phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016437">
        <property type="term" value="F:tRNA cytidylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042245">
        <property type="term" value="P:RNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001680">
        <property type="term" value="P:tRNA 3'-terminal CCA addition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00077">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05398">
        <property type="entry name" value="NT_ClassII-CCAase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.460.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01261">
        <property type="entry name" value="CCA_bact_type1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012006">
        <property type="entry name" value="CCA_bact"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003607">
        <property type="entry name" value="HD/PDEase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006674">
        <property type="entry name" value="HD_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043519">
        <property type="entry name" value="NT_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002646">
        <property type="entry name" value="PolA_pol_head_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032828">
        <property type="entry name" value="PolyA_RNA-bd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01966">
        <property type="entry name" value="HD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01743">
        <property type="entry name" value="PolyA_pol"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12627">
        <property type="entry name" value="PolyA_pol_RNAbd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000813">
        <property type="entry name" value="CCA_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81301">
        <property type="entry name" value="SSF81301"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51831">
        <property type="entry name" value="HD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0511">Multifunctional enzyme</keyword>
    <keyword id="KW-0533">Nickel</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0692">RNA repair</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <keyword id="KW-0819">tRNA processing</keyword>
    <feature type="chain" id="PRO_1000054290" description="Multifunctional CCA protein">
        <location>
            <begin position="1"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="domain" description="HD" evidence="2">
        <location>
            <begin position="232"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="8"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="8"/>
        </location>
        <ligand>
            <name>CTP</name>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="11"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="11"/>
        </location>
        <ligand>
            <name>CTP</name>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="21"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="23"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="91"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="91"/>
        </location>
        <ligand>
            <name>CTP</name>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="143"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="143"/>
        </location>
        <ligand>
            <name>CTP</name>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="146"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="146"/>
        </location>
        <ligand>
            <name>CTP</name>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01175"/>
        </source>
    </evidence>
    <sequence length="414" mass="45494" checksum="3386A3BF95AC6D36" modified="2006-05-30" version="1">MQVYAVGGAVRDQLLGKPSQDRDYVVVGATPADMEAAGFKPVGKDFPVFLHPHTKAEYALARTERKTAVGYKGFAFYTGADVTLEEDLVRRDLTINAMAQAVDEHDNLVGPVIDPYGGQKDLAARKFRHVSDAFAEDPVRILRVARFAARFHEFTVAPETVALMRKIVDAGEVDALVPERVWQELSRGLMEAKPSRMFDVLRECGALAHLLPELDRLWGVPQRPEYHPEVDTGVHVMMVIDYAASVGASLPVRFAAMAHDLGKGTTPEDMLPRHIGHEQRGVKLLEAICQRLRVPNDCRELALVVAREHGNIHRSTEFGAAALTRLVERCDALRKPERFAEALLACEADARGRLGFADKDYPQADRLLAARDAAASVDAGAIAKACGDKVDQIKDRVHKARVAAVAQRLGMAEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LS39</accession>
    <name>Q1LS39_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Predicted esterase of the alpha-beta hydrolase superfamily</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Rmet_0151</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07037.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515064.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0151"/>
    <dbReference type="EnsemblBacteria" id="ABF07037">
        <property type="protein sequence ID" value="ABF07037"/>
        <property type="gene ID" value="Rmet_0151"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0151"/>
    <dbReference type="eggNOG" id="COG1752">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_042894_0_0_4"/>
    <dbReference type="OMA" id="YFDNTRQ"/>
    <dbReference type="OrthoDB" id="1149616at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016035">
        <property type="entry name" value="Acyl_Trfase/lysoPLipase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021095">
        <property type="entry name" value="DUF3734"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002641">
        <property type="entry name" value="PNPLA_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12536">
        <property type="entry name" value="DUF3734"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01734">
        <property type="entry name" value="Patatin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52151">
        <property type="entry name" value="SSF52151"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51635">
        <property type="entry name" value="PNPLA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="1 2" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PNPLA" evidence="4">
        <location>
            <begin position="69"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="GXGXXG" evidence="2">
        <location>
            <begin position="73"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="GXSXG" evidence="2">
        <location>
            <begin position="100"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="DGA/G" evidence="2">
        <location>
            <begin position="272"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="2">
        <location>
            <position position="102"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="2">
        <location>
            <position position="272"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABF07037.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="442" mass="49228" checksum="E90927A6C6E94113" modified="2006-05-30" version="1">MAVKRRNKEAIQREIGEQGGPPQDSSSPATPERAKRGASARGSVLSEHCGQPVEPGDGPPHEAYAIRALVLQGGGALGSYQAGVYQGLAEGGIHPNWIAGISIGALNAAVIAGNPPETRVAQLRAFWEYICAQPWLPGLPLEMLSDSAPTWPEPMRIWFDSFNAARAIVEGQRGFFSPRAVTDLWAHFEDPTRVSYYDTAPLKSTLERFADFDRINHPKGMRVSVGAVNVHTGNFAYFDNTRTTLRPEHFMASGALPPGFPAIEIDGEYYWDGGLVSNTPLQEVLTAQPRRDALIFQVDLWSARGGLPHNLQDVVERQKDIQYSSRTRAITDYMREQQNYRRMLGEVLALVPQSKRNDPWYQRAADHACDARRNVIQLIYRDKSFEGSAKDYQFGVLTMHEHWASGLEDIRETLKHPKWLAMPGPDHPFVTHDVHRGNGGND</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LS83</accession>
    <name>Q1LS83_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Short-chain dehydrogenase/reductase SDR</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_0107</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF06993.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515027.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0107"/>
    <dbReference type="EnsemblBacteria" id="ABF06993">
        <property type="protein sequence ID" value="ABF06993"/>
        <property type="gene ID" value="Rmet_0107"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0107"/>
    <dbReference type="eggNOG" id="COG1028">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010194_9_1_4"/>
    <dbReference type="OMA" id="GIGLEYC"/>
    <dbReference type="OrthoDB" id="968232at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF06993.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="226" mass="23809" checksum="D78C063D9981DC46" modified="2006-05-30" version="1">MPTALVLGASRGLGLEFVRQYRADGWRVIAAARSDEGVAALTALGAEAHKLDLTDAAAVAGLAWKLDGETLDVAIYNAGVIGPRSETAEPVTREEFDAVMHTNVLGPMMALPVLLPFVESGNHGRGGVLAVLSSRMGSIGAMDSNRSWLYRVSKASVNAALKSVSLDARHAICVALHPGWVQTDMGGPKADLTPQQSVAGMRRVLAGVARHDNGSFHNYDGTSIPW</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LSA9</accession>
    <name>Q1LSA9_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">2-Oxopent-4-enoate hydratase</fullName>
            <ecNumber evidence="3">4.2.1.80</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">mhpD2</name>
        <name evidence="3" type="ordered locus">Rmet_0081</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EC" id="4.2.1.80" evidence="3"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF06967.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0081"/>
    <dbReference type="EnsemblBacteria" id="ABF06967">
        <property type="protein sequence ID" value="ABF06967"/>
        <property type="gene ID" value="Rmet_0081"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0081"/>
    <dbReference type="eggNOG" id="COG0179">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_050185_0_0_4"/>
    <dbReference type="OMA" id="QLIAHIC"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008684">
        <property type="term" value="F:2-oxopent-4-enoate hydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.850.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041072">
        <property type="entry name" value="FAA_hydro_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011234">
        <property type="entry name" value="Fumarylacetoacetase-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036663">
        <property type="entry name" value="Fumarylacetoacetase_C_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18288">
        <property type="entry name" value="FAA_hydro_N_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01557">
        <property type="entry name" value="FAA_hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56529">
        <property type="entry name" value="SSF56529"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0456">Lyase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAA_hydro_N_2" evidence="2">
        <location>
            <begin position="21"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="domain" description="FAA_hydrolase" evidence="1">
        <location>
            <begin position="104"/>
            <end position="343"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF18288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF06967.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="346" mass="38365" checksum="E0453EAF2D9FBF3B" modified="2006-05-30" version="1">MRPRRSGRLWPIRFAYVRLRMKLATLKDGSRDGQLVVVSRDLKTAHFATDIAGKLQTVLDDWAFYAPQLQDLYDALNAGRSRHPFPFQTKDCMAPLPRAYQWADGSAYVNHVELVRKARGAEMPPEFWTDPLMYQGGSDDFIGPQDDIVCASEAFGIDFEAEVAVITGDVRMGATPDQAGEAIRLVMLANDVSLRNLIPAELGKGFGFFQSKPATAFSPVAVTPDELGDAWRERRVHRPMVVHWNSKKVGSPDCGTDMVFDFGQLIAHICRTRNVRAGSIVGSGTISNVDRSKGYCCIAEKRMIETIDGGKPETEFMKYGDAVRIEMFDAEHKSIFGAIDQVVAAP</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-03-18" modified="2022-08-03" version="99" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LSC9</accession>
    <name>LIPA_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Lipoyl synthase</fullName>
            <ecNumber evidence="1">2.8.1.8</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Lip-syn</fullName>
            <shortName evidence="1">LS</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Lipoate synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Lipoic acid synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Sulfur insertion protein LipA</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">lipA</name>
        <name type="ordered locus">Rmet_0061</name>
    </gene>
    <organism>
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>[[Fe-S] cluster scaffold protein carrying a second [4Fe-4S](2+) cluster] + 4 H(+) + N(6)-octanoyl-L-lysyl-[protein] + 2 oxidized [2Fe-2S]-[ferredoxin] + 2 S-adenosyl-L-methionine = (R)-N(6)-dihydrolipoyl-L-lysyl-[protein] + 2 5'-deoxyadenosine + [[Fe-S] cluster scaffold protein] + 4 Fe(3+) + 2 hydrogen sulfide + 2 L-methionine + 2 reduced [2Fe-2S]-[ferredoxin]</text>
            <dbReference type="Rhea" id="RHEA:16585"/>
            <dbReference type="Rhea" id="RHEA-COMP:9928"/>
            <dbReference type="Rhea" id="RHEA-COMP:10000"/>
            <dbReference type="Rhea" id="RHEA-COMP:10001"/>
            <dbReference type="Rhea" id="RHEA-COMP:10475"/>
            <dbReference type="Rhea" id="RHEA-COMP:14568"/>
            <dbReference type="Rhea" id="RHEA-COMP:14569"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17319"/>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
            <dbReference type="ChEBI" id="CHEBI:29919"/>
            <dbReference type="ChEBI" id="CHEBI:33722"/>
            <dbReference type="ChEBI" id="CHEBI:33737"/>
            <dbReference type="ChEBI" id="CHEBI:33738"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:78809"/>
            <dbReference type="ChEBI" id="CHEBI:83100"/>
            <dbReference type="EC" id="2.8.1.8"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="1">Binds 2 [4Fe-4S] clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Protein modification; protein lipoylation via endogenous pathway; protein N(6)-(lipoyl)lysine from octanoyl-[acyl-carrier-protein]: step 2/2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the radical SAM superfamily. Lipoyl synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.8.1.8" evidence="1"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF06947.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011514986.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q1LSC9"/>
    <dbReference type="SMR" id="Q1LSC9"/>
    <dbReference type="STRING" id="266264.Rmet_0061"/>
    <dbReference type="EnsemblBacteria" id="ABF06947">
        <property type="protein sequence ID" value="ABF06947"/>
        <property type="gene ID" value="Rmet_0061"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0061"/>
    <dbReference type="eggNOG" id="COG0320">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033144_2_1_4"/>
    <dbReference type="OMA" id="PYCDIDF"/>
    <dbReference type="OrthoDB" id="1184806at2"/>
    <dbReference type="UniPathway" id="UPA00538">
        <property type="reaction ID" value="UER00593"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016992">
        <property type="term" value="F:lipoate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102552">
        <property type="term" value="F:lipoyl synthase activity (acting on glycine-cleavage complex H protein"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102553">
        <property type="term" value="F:lipoyl synthase activity (acting on pyruvate dehydrogenase E2 protein)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009249">
        <property type="term" value="P:protein lipoylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00206">
        <property type="entry name" value="Lipoyl_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaB/NifB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031691">
        <property type="entry name" value="LIAS_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003698">
        <property type="entry name" value="Lipoyl_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10949">
        <property type="entry name" value="PTHR10949"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16881">
        <property type="entry name" value="LIAS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005963">
        <property type="entry name" value="Lipoyl_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00271">
        <property type="entry name" value="lipoyl_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00510">
        <property type="entry name" value="lipA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0004">4Fe-4S</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0408">Iron</keyword>
    <keyword id="KW-0411">Iron-sulfur</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000325300" description="Lipoyl synthase">
        <location>
            <begin position="1"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="domain" description="Radical SAM core" evidence="2">
        <location>
            <begin position="88"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="77"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="82"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="88"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="103"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="107"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="110"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="317"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="330" mass="36884" checksum="6610684F2FDA7F17" modified="2006-05-30" version="1">MSDAPIATSSEVTQSPADYDPTKKQKSAEKTARIPIKIVPAEKLKKPDWIRVKAATGNSRFYEIKDILRANNLVTVCEEASCPNIGECFGKGTATFMIMGDKCTRRCPFCDVGHGRPDPLDVNEPGNLARTIAQLKLNYVVITSVDRDDLRDGGAQHYVDCISQTRELSPNTRIEVLVPDFRGRLDRALDILQACPPDVMNHNMETVPRLYKQARPGADYAHSLKLLQDFKRRNPNVPTKSGLMVGLGETDEEILDVMRDMRAHDIDMLTIGQYLAPSNHHLPVTRYVHPDTFKMFEEEAYKMGFTHAAVGAMVRSSYHADQQAHQAGFA</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-03-23" modified="2020-10-07" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>D3DYH8</accession>
    <name>D3DYH8_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_6771</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ADC45348.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADC45348">
        <property type="protein sequence ID" value="ADC45348"/>
        <property type="gene ID" value="Rmet_6771"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6771"/>
    <dbReference type="HOGENOM" id="CLU_2772951_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADC45348.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="69" mass="8307" checksum="0E1FF006A1225336" modified="2010-03-23" version="1">MDRRDMKMDLFTHQAYPFLRSNWRCPSDPREKRTYFCVPMRMGWDEGTVQASVDGGRERTRFCERVRSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-10-07" version="57" xmlns="http://uniprot.org/uniprot">
    <accession>Q1L9H8</accession>
    <name>Q1L9H8_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Cupin 2 conserved barrel domain protein (0rf25)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_6339</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2 3">
        <name>pMOL28</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid pMOL28</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000355">
        <property type="protein sequence ID" value="ABF13198.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF13198">
        <property type="protein sequence ID" value="ABF13198"/>
        <property type="gene ID" value="Rmet_6339"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6339"/>
    <dbReference type="HOGENOM" id="CLU_137371_0_0_4"/>
    <dbReference type="OMA" id="AMWCLEP"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid pMOL28"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013096">
        <property type="entry name" value="Cupin_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011051">
        <property type="entry name" value="RmlC_Cupin_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07883">
        <property type="entry name" value="Cupin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51182">
        <property type="entry name" value="SSF51182"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cupin_2" evidence="1">
        <location>
            <begin position="78"/>
            <end position="147"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF07883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF13198.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="160" mass="17320" checksum="4EBDA21BC2B032AF" modified="2006-05-30" version="1">MNTDPQQRRVAPPFRAGYRQRRTLGYSCSVITSQAKLIFGVDMEMESRIFSVTEYIRPSDGEPIRSVVLETKDSAVVVWHAHPGQEITAHVHPDGQDTWTVISGEAEYYQGGGKVAHLKAGDIAIAKPGQVHGALNTSPVPFVFVSVVASGNAGFALAEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-10-07" version="50" xmlns="http://uniprot.org/uniprot">
    <accession>Q1L9N2</accession>
    <name>Q1L9N2_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">PLDc_N domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Rmet_6285</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="8 9">
        <name>pMOL28</name>
    </geneLocation>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="9">Plasmid pMOL28</plasmid>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP000355">
        <property type="protein sequence ID" value="ABF13144.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF13144">
        <property type="protein sequence ID" value="ABF13144"/>
        <property type="gene ID" value="Rmet_6285"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6285"/>
    <dbReference type="HOGENOM" id="CLU_2168944_0_0_4"/>
    <dbReference type="OMA" id="MEWITIA"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid pMOL28"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027379">
        <property type="entry name" value="CLS_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13396">
        <property type="entry name" value="PLDc_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0614">Plasmid</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="30"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="domain" description="PLDc_N" evidence="7">
        <location>
            <begin position="16"/>
            <end position="52"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF13396"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ABF13144.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="110" mass="12825" checksum="34CD657EED39C5C1" modified="2006-05-30" version="1">MKDWITIGFNFFLPAYLAFRWSGKEKRSKWAWTVACFVFSWFGLIAFALTRRGLPTVEEYARTNPGNAAGGMSCNRCGSRSIRVWREQAFIKVRQYHICNHCGTTLYRSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="72" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LBI4</accession>
    <name>Q1LBI4_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Metabolite and drug efflux pump</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">yddG</name>
        <name evidence="3" type="ordered locus">Rmet_5633</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12492.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011520036.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5633"/>
    <dbReference type="EnsemblBacteria" id="ABF12492">
        <property type="protein sequence ID" value="ABF12492"/>
        <property type="gene ID" value="Rmet_5633"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5633"/>
    <dbReference type="eggNOG" id="COG0697">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_058959_1_1_4"/>
    <dbReference type="OMA" id="VALWFHY"/>
    <dbReference type="OrthoDB" id="1515911at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000620">
        <property type="entry name" value="EamA_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00892">
        <property type="entry name" value="EamA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="37"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="68"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="96"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="124"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="161"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="189"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="213"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="248"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="274"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="domain" description="EamA" evidence="2">
        <location>
            <begin position="164"/>
            <end position="289"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF12492.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="311" mass="32878" checksum="BC2AB40EA209A3F6" modified="2006-05-30" version="1">MAVTRNSKQATLIGLVAVLFWSAIVGLIRGVSQSFGATGGAALIYTVASALLWLTVGPPRTRALPRIYLLWGSVLFVSYELCLSLSIGYANTGRQAIEVGMVNYLWPSFTMLAAILFNRQRANWLILPGFLVALLGIASVLGGDQGLDLPSMAANIQDNPLSYGLAFAGAMIWAGYCTVTSRYAQGKNAVTLFFMLTALALWGKYLFTDGETMVLSMTGAIYLLLAACAMGFGYAAWNVGILHGNMTVLAGASYFIPVLSAALAATLLHAPLSFAFWKGAAMVCAGSILCWIATRGKGERAAISSGSDNPT</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LBL7</accession>
    <name>Q1LBL7_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_5600</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12459.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5600"/>
    <dbReference type="EnsemblBacteria" id="ABF12459">
        <property type="protein sequence ID" value="ABF12459"/>
        <property type="gene ID" value="Rmet_5600"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5600"/>
    <dbReference type="eggNOG" id="COG0666">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000134_18_1_4"/>
    <dbReference type="OMA" id="TARMFGK"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13637">
        <property type="entry name" value="Ank_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01415">
        <property type="entry name" value="ANKYRIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="SSF48403"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="repeat" description="ANK" evidence="1">
        <location>
            <begin position="52"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="1">
        <location>
            <begin position="85"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="1">
        <location>
            <begin position="118"/>
            <end position="150"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF12459.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="170" mass="17910" checksum="5E0AFB4361DE6F16" modified="2006-05-30" version="1">MMEQPQATMSAEDEARMIAALGQVFDLARNGDAVALAALIERGMPPNLRNEKGDTLVMLAAYHGHANALRTLLEVGASPDIRNAMGQTPIAGAAYKGYTEIVEILLEHGADVEGASPDGRTALMTAAMFQRTDIVDILKAHGADPNARDNRGMTAADAAAFMAGRHGSTH</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LBU2</accession>
    <name>Q1LBU2_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Alpha-methylacyl-CoA racemase</fullName>
            <ecNumber evidence="1">5.1.99.4</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_5525</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EC" id="5.1.99.4" evidence="1"/>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12384.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519928.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5525"/>
    <dbReference type="EnsemblBacteria" id="ABF12384">
        <property type="protein sequence ID" value="ABF12384"/>
        <property type="gene ID" value="Rmet_5525"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5525"/>
    <dbReference type="eggNOG" id="COG1804">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033975_5_0_4"/>
    <dbReference type="OMA" id="YEVYECA"/>
    <dbReference type="OrthoDB" id="969868at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008111">
        <property type="term" value="F:alpha-methylacyl-CoA racemase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1540.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10540">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003673">
        <property type="entry name" value="CoA-Trfase_fam_III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044855">
        <property type="entry name" value="CoA-Trfase_III_dom3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023606">
        <property type="entry name" value="CoA-Trfase_III_dom_1_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02515">
        <property type="entry name" value="CoA_transf_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89796">
        <property type="entry name" value="SSF89796"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0413">Isomerase</keyword>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF12384.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="378" mass="40478" checksum="0F5CBD0721AA694C" modified="2006-05-30" version="1">MGPLKGIKVVEMAGIAPGPFAAMMLADMGAEVLCIERPTAAQHCQPTASNVLHRNRRSLALDLKNPVALRVLRRLISSANALIEGYRPGVMERLGLGPEICLADNPSLVYGRMTGWGQDGPMAQAAGHDVNYISLTGALHAIGRRDAPPPIPLNLVGDFGGGGMFLAFGVVCALLEAQTSGLGQVVDAAMVDGAAAMMAYMCAARASGRWSDQRGENVLDGAAPWYRVYETSDGQYISVAAAEPKFYAELLRLLELSDETLPDQFDRSGWPTLTERLTTVFGTRSRAEWSKLLEGTDACFAPVLSLAEAPGHPHLRARNTFIEVDGIIQPAPAPRFSRTVPAQPLPWTPAKTDDRATLTAWGIDEYEISQLESAGILG</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LBV3</accession>
    <name>Q1LBV3_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Cyclopentanol dehydrogenase (Short-chain dehydrogenase/reductase SDR)</fullName>
            <ecNumber evidence="1">1.1.1.163</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">cpnA</name>
        <name evidence="1" type="ordered locus">Rmet_5514</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EC" id="1.1.1.163" evidence="1"/>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12373.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519917.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5514"/>
    <dbReference type="EnsemblBacteria" id="ABF12373">
        <property type="protein sequence ID" value="ABF12373"/>
        <property type="gene ID" value="Rmet_5514"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5514"/>
    <dbReference type="eggNOG" id="COG1028">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010194_1_0_4"/>
    <dbReference type="OMA" id="WEVANVI"/>
    <dbReference type="OrthoDB" id="1356861at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055041">
        <property type="term" value="F:cyclopentanol dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00061">
        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF12373.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="264" mass="27472" checksum="2E93C923B1C9AA79" modified="2006-05-30" version="1">MQLEKKVVLVTGAASGIGRQTCVTLAAAGARVFASDLNEEGAAQTVALIRDAGGVADSLAHDVTEESAWHAVVQKIESASGRLDGLVNSAGLMVVRPFLETDLALYRRQQAVNVDSVWLGCQAVCKLMTKTAAEFGSASIVNLSSILGLKGGSMHSAYCTSKGAVRLLTKALAVELGRSRIRVNSVHPGLVETPLGLGSMKDMISHGVPLGSLDAAVDMVAHRTPLGRWAQPVDVANVIAFLCADESRFVTGTELTVDGGYSAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-10-07" version="63" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LBX8</accession>
    <name>Q1LBX8_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative p-hydroxybenzoate efflux system component</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_5489</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12348.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5489"/>
    <dbReference type="EnsemblBacteria" id="ABF12348">
        <property type="protein sequence ID" value="ABF12348"/>
        <property type="gene ID" value="Rmet_5489"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5489"/>
    <dbReference type="eggNOG" id="COG1289">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2635497_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006726">
        <property type="entry name" value="PHBA_efflux_AaeB/fusaric-R"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04632">
        <property type="entry name" value="FUSC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="9"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="33"/>
            <end position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF12348.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="77" mass="8521" checksum="B80154315EF4CE10" modified="2006-05-30" version="1">MSGATRSKAVYRLIGTLLGAAATVALVPNLVQMPELLCLAMAWWVSLCLYISLQDRTPCSYIFTLAGYTGKRPWQLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="70" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LC82</accession>
    <name>Q1LC82_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">AAA_28 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_5385</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12244.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519790.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5385"/>
    <dbReference type="EnsemblBacteria" id="ABF12244">
        <property type="protein sequence ID" value="ABF12244"/>
        <property type="gene ID" value="Rmet_5385"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5385"/>
    <dbReference type="eggNOG" id="COG3911">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_114480_0_0_4"/>
    <dbReference type="OMA" id="TPPWPEI"/>
    <dbReference type="OrthoDB" id="1392851at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038727">
        <property type="entry name" value="NadR/Ttd14_AAA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13521">
        <property type="entry name" value="AAA_28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA_28" evidence="1">
        <location>
            <begin position="7"/>
            <end position="182"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13521"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF12244.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="192" mass="21056" checksum="68E81AED8FAA4E24" modified="2006-05-30" version="1">MTAMSQCVVISGCSGGGKSSLLSELGARGYPVVEEPGRRVVVEETARHGNALPWVDMPAFLRRVIAMARADVLALEQSAGRIGLSEPGEPGKWIFFDRGLVDAFVALQHVSGKPQLAPLVHQVRPYHRVVFLTPPWPEIYVQDAERRHDLDAATAEYARLLEAYPALGYDVRIIPRASIAERADFVLNTLQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LCP1</accession>
    <name>Q1LCP1_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_5226</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12085.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519631.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5226"/>
    <dbReference type="EnsemblBacteria" id="ABF12085">
        <property type="protein sequence ID" value="ABF12085"/>
        <property type="gene ID" value="Rmet_5226"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5226"/>
    <dbReference type="eggNOG" id="COG5361">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027269_1_1_4"/>
    <dbReference type="OMA" id="MNANLEV"/>
    <dbReference type="OrthoDB" id="547506at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.600">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1610">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010621">
        <property type="entry name" value="DUF1214"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037049">
        <property type="entry name" value="DUF1214_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010679">
        <property type="entry name" value="DUF1254"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037050">
        <property type="entry name" value="DUF1254_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06742">
        <property type="entry name" value="DUF1214"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06863">
        <property type="entry name" value="DUF1254"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF1254" evidence="2">
        <location>
            <begin position="83"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="domain" description="DUF1214" evidence="1">
        <location>
            <begin position="352"/>
            <end position="464"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF06742"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF06863"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF12085.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="480" mass="52960" checksum="183AAB184AE8E375" modified="2006-05-30" version="1">MSMKDLLVAALLSVFALSGCQSVKIGPATEQATDERAASAVNEAEVRSIAREAYIYGYPVVEAYKTLYAQAVDQGGPNFKAPFNQIGNTANVFTPNDTAIITPNSDTPYSFVWMDLRAEPLVLTLPPIESKRYYSVQLIDLYTHNFAYLGTRTSGNQGGNYMIVGPDWSGEKPANVKAVIRSESSIAYAIYRTQLFNAKDLRRVRAIQNGYKVRTLSAFLGKPAPAPASPIDWPKPVADATDSLAFFRYLNFMLQFAPTVPSEQDVMARFAKIGVGRGLPFDKNSLSPAIQEAMAGGMADGKSQFVEFKKSQLDTRKLTSGDLFGTREHLKNNYMYRYAAAVLGIFGNSAEEAIYPGYFLDAAGKPLDAAATRYTLHFDKDKLPPADAFWSLTMYDGQSKLLVANPLNRYLINASMLGQLKRDRDGGLTLYVQKDNPGKDKASNWLPAPDGSFYAILRVYLPKQEMLSGQWRQPPMVVAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="56" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LCT7</accession>
    <name>Q1LCT7_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_5180</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12039.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5180"/>
    <dbReference type="EnsemblBacteria" id="ABF12039">
        <property type="protein sequence ID" value="ABF12039"/>
        <property type="gene ID" value="Rmet_5180"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5180"/>
    <dbReference type="eggNOG" id="ENOG50316QR">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2635506_0_0_4"/>
    <dbReference type="OMA" id="FTERWAY"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF12039.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="82" mass="9470" checksum="782BC3C4432CFDCD" modified="2006-05-30" version="1">MEMSMQRTTEYRGFAIRIDLLNTSEGMFDTWFQIKGPIRTRDAVYGARLKVLGSPFSRRWAHLVGELAARASIDQILDVDEY</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-12-12" modified="2022-05-25" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LCU1</accession>
    <name>IF13_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Translation initiation factor IF-1 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">infA3</name>
        <name type="ordered locus">Rmet_5176</name>
    </gene>
    <organism>
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">One of the essential components for the initiation of protein synthesis. Stabilizes the binding of IF-2 and IF-3 on the 30S subunit to which N-formylmethionyl-tRNA(fMet) subsequently binds. Helps modulate mRNA selection, yielding the 30S pre-initiation complex (PIC). Upon addition of the 50S ribosomal subunit IF-1, IF-2 and IF-3 are released leaving the mature 70S translation initiation complex.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Component of the 30S ribosomal translation pre-initiation complex which assembles on the 30S ribosome in the order IF-2 and IF-3, IF-1 and N-formylmethionyl-tRNA(fMet); mRNA recruitment can occur at any time during PIC assembly.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the IF-1 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12035.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008646230.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q1LCU1"/>
    <dbReference type="SMR" id="Q1LCU1"/>
    <dbReference type="STRING" id="266264.Rmet_5176"/>
    <dbReference type="EnsemblBacteria" id="ABF12035">
        <property type="protein sequence ID" value="ABF12035"/>
        <property type="gene ID" value="Rmet_5176"/>
    </dbReference>
    <dbReference type="GeneID" id="60824843"/>
    <dbReference type="KEGG" id="rme:Rmet_5176"/>
    <dbReference type="eggNOG" id="COG0361">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_151267_4_1_4"/>
    <dbReference type="OMA" id="VLLENDH"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043022">
        <property type="term" value="F:ribosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd04451">
        <property type="entry name" value="S1_IF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00075">
        <property type="entry name" value="IF_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006196">
        <property type="entry name" value="RNA-binding_domain_S1_IF1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004368">
        <property type="entry name" value="TIF_IF1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33370">
        <property type="entry name" value="PTHR33370"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01176">
        <property type="entry name" value="eIF-1a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00008">
        <property type="entry name" value="infA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50832">
        <property type="entry name" value="S1_IF1_TYPE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0396">Initiation factor</keyword>
    <keyword id="KW-0614">Plasmid</keyword>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <feature type="chain" id="PRO_0000263851" description="Translation initiation factor IF-1 3">
        <location>
            <begin position="1"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="domain" description="S1-like" evidence="1">
        <location>
            <begin position="1"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00075"/>
        </source>
    </evidence>
    <sequence length="73" mass="8472" checksum="DA3295E8B832B9C7" modified="2006-05-30" version="1">MAKEELVEFGGKVSEVLPDNRFRVILENGFEVWAYSSGRLKKNRIRVLAGDRVTLEMSPYDLTKGRINYRHKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="111" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LCY7</accession>
    <name>Q1LCY7_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Two component transcriptional regulator, OmpR/winged helix family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Rmet_5130</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="6">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="6">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11989.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519536.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5130"/>
    <dbReference type="EnsemblBacteria" id="ABF11989">
        <property type="protein sequence ID" value="ABF11989"/>
        <property type="gene ID" value="Rmet_5130"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5130"/>
    <dbReference type="eggNOG" id="COG0745">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_30_1_4"/>
    <dbReference type="OMA" id="HHQQVVP"/>
    <dbReference type="OrthoDB" id="1020672at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00383">
        <property type="entry name" value="trans_reg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001867">
        <property type="entry name" value="OmpR/PhoB-type_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039420">
        <property type="entry name" value="WalR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48111">
        <property type="entry name" value="PTHR48111"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00486">
        <property type="entry name" value="Trans_reg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00862">
        <property type="entry name" value="Trans_reg_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51755">
        <property type="entry name" value="OMPR_PHOB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="5" id="KW-0614">Plasmid</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Response regulatory" evidence="3">
        <location>
            <begin position="2"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="domain" description="OmpR/PhoB-type" evidence="4">
        <location>
            <begin position="124"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="OmpR/PhoB-type" evidence="2">
        <location>
            <begin position="124"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="1">
        <location>
            <position position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABF11989.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="223" mass="24468" checksum="37A5B3C987A782AA" modified="2006-05-30" version="1">MRILLIEDEATLARQLADTLSQAGYVVDHASDGIDAEHLGTENPYDAVILDLGLPKLDGLTVLKRWRQAGNGVPVLILTARDSWVEKVEGIDAGADDYLTKPFRAEELLARIRALIRRSTGHTSPVLVCGPVTLAPATGQVSVDGSPIVLTAHEYKVLDYLMHHQGVIVSKTTLTEHIYAQDHDRDSNTIEVFVGRLRRKLGVDVIETVRGLGYRLRPPEHGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LD02</accession>
    <name>Q1LD02_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Lysine exporter protein (LYSE/YGGA)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Rmet_5115</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="7">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="7">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11974.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519521.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5115"/>
    <dbReference type="EnsemblBacteria" id="ABF11974">
        <property type="protein sequence ID" value="ABF11974"/>
        <property type="gene ID" value="Rmet_5115"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5115"/>
    <dbReference type="eggNOG" id="COG1280">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_079569_0_0_4"/>
    <dbReference type="OMA" id="SVIWRVF"/>
    <dbReference type="OrthoDB" id="1927576at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006865">
        <property type="term" value="P:amino acid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001123">
        <property type="entry name" value="LeuE-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30086">
        <property type="entry name" value="PTHR30086"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01810">
        <property type="entry name" value="LysE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0614">Plasmid</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="6"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="40"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="68"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="125"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="147"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="182"/>
            <end position="201"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABF11974.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="203" mass="20980" checksum="DAEC2367FE8A58DC" modified="2006-05-30" version="1">MEYINTLLALTGVMMLSVASPGPNFMIVTSTAVASRRAGVITGLALGAASGTWALIAIAGLGLIVTHVAWIGTALRIAGAAYLIWLGAKMILTARHPLNAPTLVAPSGWTAAKKGYAVSMTNPKAVAFYGSIFALMVPAHAPAWFHVAVIAIAVAVSSAWYCGMALLASHPAVHRLLMRRKAVLDSVVGGLLIVLGGRMLAAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="111" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LD25</accession>
    <name>Q1LD25_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Oligopeptide/dipeptide ABC transporter, ATP-binding</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">dppE</name>
        <name evidence="10" type="ordered locus">Rmet_5092</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="11">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="11">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11951.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519497.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5092"/>
    <dbReference type="EnsemblBacteria" id="ABF11951">
        <property type="protein sequence ID" value="ABF11951"/>
        <property type="gene ID" value="Rmet_5092"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5092"/>
    <dbReference type="eggNOG" id="COG4608">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_23_4"/>
    <dbReference type="OMA" id="CPIAMDV"/>
    <dbReference type="OrthoDB" id="1303270at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015833">
        <property type="term" value="P:peptide transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013563">
        <property type="entry name" value="Oligopep_ABC_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08352">
        <property type="entry name" value="oligo_HPY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01727">
        <property type="entry name" value="oligo_HPY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 8 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-0614">Plasmid</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-1278">Translocase</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ABC transporter" evidence="9">
        <location>
            <begin position="18"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="61"/>
            <end position="68"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ABF11951.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="337" mass="36957" checksum="FFD1F058ED4198A6" modified="2006-05-30" version="1">MNPSQEPIAATAQAAPLLQVDGLRKHYTAPRRWPSPPPRPIQAVDNVSFTVARGETLSLVGESGCGKTTTAKSVLRLVEPTAGSVRLDGEELLTLSAGEMRRRRRELQIIFQDPYASLSPRLTAGEIVAEPLRNFGMRSRAEREARVQWLFGKVGLRPEATAKYPHEFSGGQRQRLGIARALAMQPKLIVCDEPVSALDVSVQAQVVNLLMDLQQEFGIAYLFVAHDLAVVRHISRRVAVMYLGRIVELADRDALFSTPRHPYTEILLSAAPVPNPRAPARRILLQGDPPSPANPPSGCHFHTRCPLAQPICREQAPALTARPSDAPGGHQVACHFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="66" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LD84</accession>
    <name>Q1LD84_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Protein involved in copper, silver resistance CusD</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">cusD</name>
        <name evidence="2" type="ordered locus">Rmet_5030</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11892.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519443.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5030"/>
    <dbReference type="EnsemblBacteria" id="ABF11892">
        <property type="protein sequence ID" value="ABF11892"/>
        <property type="gene ID" value="Rmet_5030"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5030"/>
    <dbReference type="eggNOG" id="ENOG5033WHC">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_121434_0_0_4"/>
    <dbReference type="OMA" id="HAILHCC"/>
    <dbReference type="OrthoDB" id="1970047at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193078" evidence="1">
        <location>
            <begin position="28"/>
            <end position="133"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF11892.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="133" mass="14534" checksum="1E2D348355BCB2E8" modified="2006-05-30" version="1">MRHLARRHSWIAGFLLILFLSVQLATAAYACTMGMNSMDEHPSMEATMSCADMAEQDAAQNDGQKALCMSHCQANAKHADHATPQIPAFIPFLVSIVVEPPVLDDAPMLAERAQSQPRAPPPPHAILHCCFRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LDM0</accession>
    <name>Q1LDM0_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Cyclic nucleotide-regulated FAD-dependent pyridine nucleotide-disulphide oxidoreductase</fullName>
            <ecNumber evidence="3">1.8.1.9</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_4894</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EC" id="1.8.1.9" evidence="3"/>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11756.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4894"/>
    <dbReference type="EnsemblBacteria" id="ABF11756">
        <property type="protein sequence ID" value="ABF11756"/>
        <property type="gene ID" value="Rmet_4894"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4894"/>
    <dbReference type="eggNOG" id="COG0492">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG0664">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031864_5_8_4"/>
    <dbReference type="OMA" id="WSGRAYE"/>
    <dbReference type="OrthoDB" id="692968at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004791">
        <property type="term" value="F:thioredoxin-disulfide reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd00038">
        <property type="entry name" value="CAP_ED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018490">
        <property type="entry name" value="cNMP-bd-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023753">
        <property type="entry name" value="FAD/NAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00027">
        <property type="entry name" value="cNMP_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07992">
        <property type="entry name" value="Pyr_redox_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00100">
        <property type="entry name" value="cNMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51206">
        <property type="entry name" value="SSF51206"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="SSF51905"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="2">
        <location>
            <begin position="43"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF11756.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="588" mass="63023" checksum="AA5FB15083702E70" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LDX3</accession>
    <name>Q1LDX3_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Putative secretion protein HlyD family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Rmet_4791</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="6">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="6">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11653.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519220.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4791"/>
    <dbReference type="EnsemblBacteria" id="ABF11653">
        <property type="protein sequence ID" value="ABF11653"/>
        <property type="gene ID" value="Rmet_4791"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4791"/>
    <dbReference type="eggNOG" id="COG1566">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018816_15_2_4"/>
    <dbReference type="OMA" id="EVEPTFN"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039562">
        <property type="entry name" value="MFP_biotin_lipoyl_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006143">
        <property type="entry name" value="RND_pump_MFP"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13533">
        <property type="entry name" value="Biotin_lipoyl_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01730">
        <property type="entry name" value="RND_mfp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0614">Plasmid</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Biotin_lipoyl_2" evidence="4">
        <location>
            <begin position="46"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="287"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="87"/>
            <end position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF13533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABF11653.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="328" mass="35470" checksum="3A23616043FD02E1" modified="2006-05-30" version="1">MKLRLSSLGPVVLTLAVTAVGAVVVKHLWDYYTVAPWTRDGHIRAEVVQVAPDVSGLVTRIAVKDNQHVKAGDVLFEIDHERYALALRQAEATAAAVRANLAQARREAKRSQTLSEVVSKEIVEEGLAKVQQGEAQLAQAEAAIGVAKLNLERTHVVSPVEGYVNDRLPRLGDYVVTGKPVLSMVDSNSFHVEGYFEETKLKGIHIGSPVDIRVMGEKRVLHGHVQSIAAGIEDRDRTNGSNLLPNVNPTFNWVRLAQRVPVRIQFDQMPEDVRLVAGRTATVSVKADDEKNIGETGDKPTAPAAGRSASQDKVALAGKRAPSTGAVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="62" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LE07</accession>
    <name>Q1LE07_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_4757</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11619.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519191.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4757"/>
    <dbReference type="EnsemblBacteria" id="ABF11619">
        <property type="protein sequence ID" value="ABF11619"/>
        <property type="gene ID" value="Rmet_4757"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4757"/>
    <dbReference type="eggNOG" id="ENOG502ZM54">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1394294_0_0_4"/>
    <dbReference type="OMA" id="QRWLPLM"/>
    <dbReference type="OrthoDB" id="1593220at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193385" evidence="1">
        <location>
            <begin position="27"/>
            <end position="196"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF11619.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="196" mass="20621" checksum="0B691A2A96FBD69B" modified="2006-05-30" version="1">MFSRFISRAALLAGACLCLATPIAYAESDGMGPMTPPMPNPAVRPVAAGALYQCQGPNGGTVFRASPREGCTLVASPDPAAPDPQRWMPLMGATGMVSYLDQTAIRRRGTEVGVVLVHNAPAGVIKTASGDTIRSSLKRMVLNCATSMYAVIEQTLYNKRYARGESLYTIRGPQAGMPQPAAPGTLAGELIGRLCH</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LE23</accession>
    <name>Q1LE23_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Putative 2OG-Fe(II) oxygenase Flavanone 3-dioxygenase / Isopenicillin-N synthase</fullName>
            <ecNumber evidence="5">1.14.11.9</ecNumber>
            <ecNumber evidence="5">1.21.3.1</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Rmet_4741</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="6">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="6">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the iron/ascorbate-dependent oxidoreductase family.</text>
    </comment>
    <dbReference type="EC" id="1.14.11.9" evidence="5"/>
    <dbReference type="EC" id="1.21.3.1" evidence="5"/>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11603.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519175.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4741"/>
    <dbReference type="EnsemblBacteria" id="ABF11603">
        <property type="protein sequence ID" value="ABF11603"/>
        <property type="gene ID" value="Rmet_4741"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4741"/>
    <dbReference type="eggNOG" id="COG3491">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010119_6_2_4"/>
    <dbReference type="OMA" id="FKEAMNF"/>
    <dbReference type="OrthoDB" id="400496at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016216">
        <property type="term" value="F:isopenicillin-N synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045486">
        <property type="term" value="F:naringenin 3-dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026992">
        <property type="entry name" value="DIOX_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044861">
        <property type="entry name" value="IPNS-like_FE2OG_OXY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027443">
        <property type="entry name" value="IPNS-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005123">
        <property type="entry name" value="Oxoglu/Fe-dep_dioxygenase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03171">
        <property type="entry name" value="2OG-FeII_Oxy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14226">
        <property type="entry name" value="DIOX_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51471">
        <property type="entry name" value="FE2OG_OXY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 5" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="2" id="KW-0408">Iron</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2 5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-0614">Plasmid</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fe2OG dioxygenase" evidence="4">
        <location>
            <begin position="183"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU003682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABF11603.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="330" mass="37487" checksum="45D7FD08E395BE69" modified="2006-05-30" version="1">MTNATLPLHELARESRMGAHGSETDTREIRCIDLSDFDTRKPEITDQLWRAATDIGFFQLRHHGIDLPAIRQAFAMAEQFFALPETVKARYPLQKANNAGWESRAQVRPSTGTPDQKESYQITRPHMAPLWPSETELPGFRQTMLEFEAQCWNVGMKVLSCFADRLGFDTDFFSRAHDPAAPDYQSTLRLLHYYAIPPEAQDKLDLWRAGAHTDFDCLTLLFQREGQGGLQVCPGKEMDAQAWTPIAPIEDVVTCNIGDMLMRWSDDHLPSNFHRVRNPAPGEYMGPRYSLAFFCQANRDAVIEGPSKRYPAITAGDFLRQRVAANFKAY</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="53" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LEE6</accession>
    <name>Q1LEE6_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_4618</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11480.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519055.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4618"/>
    <dbReference type="EnsemblBacteria" id="ABF11480">
        <property type="protein sequence ID" value="ABF11480"/>
        <property type="gene ID" value="Rmet_4618"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4618"/>
    <dbReference type="HOGENOM" id="CLU_1925843_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="43"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="67"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="106"/>
            <end position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF11480.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="131" mass="14774" checksum="D3714E0648FCCAC6" modified="2006-05-30" version="1">MHPRKWHTNLLILFLAGWSLVSLVGVSVLMIRAPVPVDPADCLLFLWTPILIGWASWLLFRQHRYQALPLCIVPAMYIMTAVRLSQGRLSFVNWRVNVWNLTSFPLGYGLCAAFFLACAFYAAHLAARASA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LEG8</accession>
    <name>Q1LEG8_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Heavy metal cation tricomponent efflux outer membrane porin (CzcC-like)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">czcC2</name>
        <name evidence="3" type="ordered locus">Rmet_4596</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the outer membrane factor (OMF) (TC 1.B.17) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11458.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4596"/>
    <dbReference type="EnsemblBacteria" id="ABF11458">
        <property type="protein sequence ID" value="ABF11458"/>
        <property type="gene ID" value="Rmet_4596"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4596"/>
    <dbReference type="eggNOG" id="COG1538">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012817_14_3_4"/>
    <dbReference type="OMA" id="YGYLDYV"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015562">
        <property type="term" value="F:efflux transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003423">
        <property type="entry name" value="OMP_efflux"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02321">
        <property type="entry name" value="OEP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193075" evidence="2">
        <location>
            <begin position="30"/>
            <end position="431"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF11458.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="431" mass="45775" checksum="26568462114F5291" modified="2006-05-30" version="1">MRNFLSPPGLAAVLLCLSLGPIVSPVASAQGTAAPAPAPVWVQVPAQEPPSALTLEAALALATAQNPTLSAARIELDSTEGGITQARVIPNPEIAVQMEDTRAATRATTGQVNLPIELGGKRSARIGAANRARDVAQAQLGTTQADLRASVIGAFFNVLVAQERVRLAAGSVDIATRGANAAARRVAAGKISPVEETKAQVELANAQIERSEADAALVAARQSLATLWGNPSPRFTEADGNLDALPARPQATMLRQSLDGAPALLASERELDRRQAEINVQRSRQYPDVTVSVGAKRDNSSPERGTYPVLGVAIPLPLFDRNQGNLYTAIRQADKAADELRATRLRLDHDLQQASSQLEVSRSSALSLRETVLPAAERAYQAASQGFEAGKFNYLEVLDAQRTLFQARIRYLGVVANSWQAATTIDRILGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LEP6</accession>
    <name>Q1LEP6_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Sensor kinase (Sensor kinase, two-component system)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Rmet_4515</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="5">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="5">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11380.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518973.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4515"/>
    <dbReference type="EnsemblBacteria" id="ABF11380">
        <property type="protein sequence ID" value="ABF11380"/>
        <property type="gene ID" value="Rmet_4515"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4515"/>
    <dbReference type="eggNOG" id="COG4585">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_114_0_4"/>
    <dbReference type="OMA" id="VVLFNPM"/>
    <dbReference type="OrthoDB" id="1755994at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00130">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000014">
        <property type="entry name" value="PAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000700">
        <property type="entry name" value="PAS-assoc_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035965">
        <property type="entry name" value="PAS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013767">
        <property type="entry name" value="PAS_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011712">
        <property type="entry name" value="Sig_transdc_His_kin_sub3_dim/P"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07730">
        <property type="entry name" value="HisKA_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00989">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00091">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55785">
        <property type="entry name" value="SSF55785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="SSF55874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00229">
        <property type="entry name" value="sensory_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50113">
        <property type="entry name" value="PAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50112">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="4" id="KW-0614">Plasmid</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PAS" evidence="2">
        <location>
            <begin position="20"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="domain" description="PAC" evidence="3">
        <location>
            <begin position="98"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="140"/>
            <end position="167"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF11380.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="383" mass="42265" checksum="DDD91E883226F624" modified="2006-05-30" version="1">MGPEAVTGTDDNGDRRIAENETRLAGIIRSSMEAIISVDEEQRVVLFNPMAEKLFGLPAEQAMGRPLGDFIPARFRTAHAAHVTRFGVTGVSDRQMGRQLTLHALRQDGTEFPIEASISQTSDGAGHKLFTVMLRDVTARVRAETALQRSREELQALSDSILAAREDEKRRVARELHDDLGQRLSALKMDLVMLETDLRTDCGAVRALGQVSAMHTVIDETIAAVRRISSDLRPALLDELGLAAALDWLAKDFSRRYAIPVSTRTPEEIDVTEQAATAVFRIVQEALNNVVHHADASEAWVTLTHQNPNQNHDHDQYVLRVHDDGRGWNGQPKDGARRSFGLLGIRERARLLGGTVSIEHSPGEGFELVVTFPGHPDCKETQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="66" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LEP8</accession>
    <name>Q1LEP8_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Beta-hydroxyacyl-(Acyl-carrier-protein) dehydratase, FabA/FabZ</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">fabA</name>
        <name evidence="1" type="ordered locus">Rmet_4513</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11378.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518971.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4513"/>
    <dbReference type="EnsemblBacteria" id="ABF11378">
        <property type="protein sequence ID" value="ABF11378"/>
        <property type="gene ID" value="Rmet_4513"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4513"/>
    <dbReference type="eggNOG" id="COG0764">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_983083_0_0_4"/>
    <dbReference type="OrthoDB" id="1763197at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013114">
        <property type="entry name" value="FabA_FabZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029069">
        <property type="entry name" value="HotDog_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30272">
        <property type="entry name" value="PTHR30272"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07977">
        <property type="entry name" value="FabA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54637">
        <property type="entry name" value="SSF54637"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF11378.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="283" mass="30202" checksum="304DA84CF122811D" modified="2006-05-30" version="1">MSHSEIPCAATALRDQLFVSGTDLFALGHYPGNPVYPGALMLDRMLIMAAALAGQAFGQEAWARRVKRVQYLGVVVPGDVVGLDVSIVERDGDQVTCLAVAHVQGNARVQATIVCVPNAEPVSAPLECDVASEAPALTHPDIARLLPHRYPFLLLDTVRSFTPREHILATKVINRESPILGAHPPAAYPASLAIESFGQAGVTLFCLSQDNASPAQVLVGLMRDVTVHRTIPYDTVLTLDVRIDRLRSNAVIFGGDIRIGEESVIHVGSLVVMIGPEQPLAGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LFQ0</accession>
    <name>Q1LFQ0_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">pelE</name>
        <name evidence="2" type="ordered locus">Rmet_4159</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11026.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518648.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4159"/>
    <dbReference type="EnsemblBacteria" id="ABF11026">
        <property type="protein sequence ID" value="ABF11026"/>
        <property type="gene ID" value="Rmet_4159"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4159"/>
    <dbReference type="eggNOG" id="COG0457">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_061179_1_0_4"/>
    <dbReference type="OMA" id="LYWELIY"/>
    <dbReference type="OrthoDB" id="1808875at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="29"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="63"/>
            <end position="87"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF11026.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="328" mass="36151" checksum="80110CD354972CF0" modified="2006-05-30" version="1">MGKLTFGGAAFQISALALLFSGGTGLPLLLGFLTLQGAAAALLGLVLWRLLPRRFRTPFVWSYGYLAAFCFFVPVGGILVCVGSLLFSKLFPRRGSNSGIASVALPEFVTHLIQRVTHGGGARLRAQLGNSRAPLPERMTALVAMQSMPTRTASPMLRELLADSTDDIRLLAYGMLDGAEKQLTQKILAELPRLESADSPQARGEINQRLADLYWELIYQNLVQGDVYRYTASQVERYASAALEIDGNIAALWYMRGRLALTRNAPAEAREFLARAEALGFARDRVLPLLAEAAYLERDYATVRRLMADFDSPSPLPLVRPLLRYWQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LG58</accession>
    <name>Q1LG58_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">FecR-like transmembrane sensor, Fe2+-dicitrate sensor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">fecR</name>
        <name evidence="3" type="ordered locus">Rmet_4000</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10868.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4000"/>
    <dbReference type="EnsemblBacteria" id="ABF10868">
        <property type="protein sequence ID" value="ABF10868"/>
        <property type="gene ID" value="Rmet_4000"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4000"/>
    <dbReference type="eggNOG" id="COG3712">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_050192_0_0_4"/>
    <dbReference type="OMA" id="RYWVRVE"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006860">
        <property type="entry name" value="FecR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032623">
        <property type="entry name" value="FecR_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012373">
        <property type="entry name" value="Ferrdict_sens_TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30273">
        <property type="entry name" value="PTHR30273"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16220">
        <property type="entry name" value="DUF4880"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04773">
        <property type="entry name" value="FecR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF018266">
        <property type="entry name" value="FecR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <feature type="domain" description="DUF4880" evidence="2">
        <location>
            <begin position="4"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="domain" description="FecR" evidence="1">
        <location>
            <begin position="105"/>
            <end position="199"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF04773"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF16220"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF10868.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="314" mass="33824" checksum="4DB3F4F09533501E" modified="2006-05-30" version="1">MALAAVEWLVTLQGGETDGASHAAWRHWLSQHPDHARAWARIEAVNARLRDVASPLTTAVAHAALTTPRSASRRRAVQALVVAFTGGAAAWAARDHAPLQDWLADVSTAVGEQRLLSLADGTQIRLNSDTAIDIAFTATERRVVLRRGEIHVQTAPDRHWPARPFIVATASATLRPMGTRFAVRVSGDAGWIAVTEGAVAVQPQADPTGGRIIAAGKQARFTPASVGAPTPLPESDTAWTDGMIVATDMRLADFLAEVDRYRPGRLRCAPDVADLRVSGTFPVADTDRILDALRTALPVRIHFLTRYWTTVLPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LG82</accession>
    <name>Q1LG82_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">ABC-type branched-chain amino acid transport systems, livK-like periplasmic component</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_3976</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10844.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518484.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3976"/>
    <dbReference type="EnsemblBacteria" id="ABF10844">
        <property type="protein sequence ID" value="ABF10844"/>
        <property type="gene ID" value="Rmet_3976"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3976"/>
    <dbReference type="eggNOG" id="COG0683">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027128_6_2_4"/>
    <dbReference type="OMA" id="HDYAKAW"/>
    <dbReference type="OrthoDB" id="776281at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028081">
        <property type="entry name" value="Leu-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028082">
        <property type="entry name" value="Peripla_BP_I"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13458">
        <property type="entry name" value="Peripla_BP_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53822">
        <property type="entry name" value="SSF53822"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193428" evidence="1">
        <location>
            <begin position="29"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="domain" description="Peripla_BP_6" evidence="2">
        <location>
            <begin position="32"/>
            <end position="342"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF10844.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="390" mass="41659" checksum="F6BF99446CEA42DC" modified="2006-05-30" version="1">MQYKTLGRFFPLAACAVAALVASAPTLAQEVVKIGYTGPLSGGAALYGKNVLSGIQMAVDEINASGLEVKGKKIKLEVVSLDDKYAPAEAAINARRLVQQYKTPAVFVPHSGGIFALQAFNEQEKFMVMAYSSVPRITDAGNKLTIRIPPAYTGYIEPFVKAQMKRFGKNVALVPADHDYAKAWVQAFVPAWEGAGGKVVGNNPMSYTKATDFYTGVSRAIADKPDVMFVGGPSEPTALVVKQARELGFKGGFIIMDQAKMDEMAKVTNGLAMLEGSIGVMPLVNDARPVAQAYNARYKKLHDGRDATTEMSLNYTMTWALANAMKLAGTTSDAAAIRAKMPEAVKTLPKDVNPNEVEGIDAKGGSMADTVVGWVQNGKIAPVRLSELGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="110" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LGC0</accession>
    <name>Q1LGC0_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Cobyrinic acid a,c-diamide synthase</fullName>
            <ecNumber evidence="6">6.3.5.9</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">cobB</name>
        <name evidence="6" type="ordered locus">Rmet_3938</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="7">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="7">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CobB/CobQ family. CobQ subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.3.5.9" evidence="6"/>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10806.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3938"/>
    <dbReference type="EnsemblBacteria" id="ABF10806">
        <property type="protein sequence ID" value="ABF10806"/>
        <property type="gene ID" value="Rmet_3938"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3938"/>
    <dbReference type="eggNOG" id="COG1797">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022752_0_2_4"/>
    <dbReference type="OMA" id="QPFKCGP"/>
    <dbReference type="OrthoDB" id="692368at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042242">
        <property type="term" value="F:cobyrinic acid a,c-diamide synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043802">
        <property type="term" value="F:hydrogenobyrinic acid a,c-diamide synthase (glutamine-hydrolysing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006541">
        <property type="term" value="P:glutamine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004484">
        <property type="entry name" value="CbiA_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002586">
        <property type="entry name" value="CobQ/CobB/MinD/ParA_Nub-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011698">
        <property type="entry name" value="GATase_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43873">
        <property type="entry name" value="PTHR43873"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01656">
        <property type="entry name" value="CbiA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07685">
        <property type="entry name" value="GATase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="SSF52317"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="6" id="KW-0436">Ligase</keyword>
    <keyword evidence="6" id="KW-0614">Plasmid</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CbiA" evidence="4">
        <location>
            <begin position="47"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="domain" description="GATase_3" evidence="5">
        <location>
            <begin position="276"/>
            <end position="463"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF07685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABF10806.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="473" mass="50257" checksum="629A82758CCED7AB" modified="2006-05-30" version="1">MSARYNPSHRMALPPCIATSEMPPLPIQSSPLGTTTRTGTRPVPALLVAAPASGQGKTTVTAALARLHARQGRTVRVFKIGPDFLDPTLLAAASGAPVQSIDLWMTGEVDARQRLALAAQTADLILVEGVMGLHDGTPSSADVARCFRLPVLAVIQAGAMAQTFGAVAHGLATYGEPFHAMHVLANGVGSARHAEMLRDSLPHDIHWAGALARDDAAALPERHLGLFSADELPDLTARLDRMADALAALPLSHLPEPIVFPDTPTQQLPRLLEGRRIAIARDDAFRFIYPANVETLNALGAHITWFSPLRDAALPHCDALWLPGGYPELHAAALAANVPMHNAIRAAHAHGLPMLAECGGMMALFDNLVDKEGQTHRMAGLLPGTVTMQKRLAAIGHQAIELPTREGMVGQPVRGHTFHYSTTDTPLTPLLHARSPRDDGTGEAVYRSGSLTASYVHLYFPSNPTAIAALFGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LGE5</accession>
    <name>Q1LGE5_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Extra-cytoplasmic solute receptor protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">bug</name>
        <name evidence="4" type="ordered locus">Rmet_3913</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="5">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="5">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Periplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the UPF0065 (bug) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10781.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518428.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3913"/>
    <dbReference type="EnsemblBacteria" id="ABF10781">
        <property type="protein sequence ID" value="ABF10781"/>
        <property type="gene ID" value="Rmet_3913"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3913"/>
    <dbReference type="eggNOG" id="COG3181">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045683_0_2_4"/>
    <dbReference type="OMA" id="GTQPHTI"/>
    <dbReference type="OrthoDB" id="447734at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042597">
        <property type="term" value="C:periplasmic space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005064">
        <property type="entry name" value="BUG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042100">
        <property type="entry name" value="Bug_dom1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42928">
        <property type="entry name" value="PTHR42928"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03401">
        <property type="entry name" value="TctC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017082">
        <property type="entry name" value="YflP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-0675">Receptor</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193472" evidence="3">
        <location>
            <begin position="30"/>
            <end position="332"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF10781.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="332" mass="35238" checksum="8AF58B91B501E710" modified="2006-05-30" version="1">MKQTIPPRRRWVMRAACAAVALLPLATFAQGSYPSKPIRMVVPYPAGGLTDTVARQLAEGLGKRLGQPVVVENRGGAGGIIGTDFVAKSPADGYTLLMTIPGPITANIALYKKLPYDPRTELRPISDVAMARTVLTVNSAVPVKTVTELIAYAKKEPGKLRMGSWGPGTQPHTIQAYMDKTYGVDILHVPYRGEGPMVTDLLAGQVNLTIGSVTTLRQYIAVGKLRPLAVAGTTRAKALPDVPTFTEAGYSDAAYKIVGPTTLLAPAKTPDAIIERLGREVSALVKSPELSAKIDELGAEPIGNTPAEAERAYKAYLPVVLKLTADTGVTLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="102" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LGI4</accession>
    <name>Q1LGI4_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Putative transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_3874</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10742.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518389.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3874"/>
    <dbReference type="EnsemblBacteria" id="ABF10742">
        <property type="protein sequence ID" value="ABF10742"/>
        <property type="gene ID" value="Rmet_3874"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3874"/>
    <dbReference type="eggNOG" id="COG1028">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010194_1_3_4"/>
    <dbReference type="OMA" id="VGTLGKQ"/>
    <dbReference type="OrthoDB" id="1356861at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF10742.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="256" mass="25851" checksum="4CEECD93CE34F2F9" modified="2006-05-30" version="1">MNSTPTLTSASAALSNKVAFVTGGSRGIGAAVVRRLAREGAAVAFTYQSSGAVAQALAESIQAAGGRARAYHADAADAAAVTRAIDAAAADFGKIDILVNNAGVLFLGPVDTFPLEDFDKTLAVNVRAVFVAAKAALAHMGQGGRIINIGSTNADRMPFPGGAAYAMSKSALKGLVQGMARDLGPKGITVNNVQPGPVNTDMNPEDSEFAASLHGLMALQRHAHPDEIASMVAYLAGPEAGFVTGASLNVDGGFAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="72" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LGI9</accession>
    <name>Q1LGI9_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative oxidoreductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_3869</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10737.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518384.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3869"/>
    <dbReference type="EnsemblBacteria" id="ABF10737">
        <property type="protein sequence ID" value="ABF10737"/>
        <property type="gene ID" value="Rmet_3869"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3869"/>
    <dbReference type="eggNOG" id="COG1902">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012153_0_0_4"/>
    <dbReference type="OMA" id="QHRIGMA"/>
    <dbReference type="OrthoDB" id="1064766at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001155">
        <property type="entry name" value="OxRdtase_FMN_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045247">
        <property type="entry name" value="Oye-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22893">
        <property type="entry name" value="PTHR22893"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00724">
        <property type="entry name" value="Oxidored_FMN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Oxidored_FMN" evidence="1">
        <location>
            <begin position="4"/>
            <end position="329"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00724"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF10737.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="359" mass="38455" checksum="BFF9754CE76D77BF" modified="2006-05-30" version="1">MSALFTPVRVGRYTLKNHLVMAPMTRSRASLDGTPGELAAEYYAQRASVGLIVTEGTQPSDDGQGYLATPGIYTEAHVDGWKNVTAAVHERGGRIFIQLMHAGRMSHPDNTPHHRQGVAPSAIAPGVPMFTLKGMQDIPAPRALTTAEVRQTVDDFRFAARQAIAAGADGVEIHGANGYLVQQFFAPNANTRTDKYGGSIDNRARFAIEVATAIAGEIGADRTAIRLSPGTPLWGIDEGAEGPALYRHLVTELDKLGLAYLHVSLQGNEPQLADIRERWRGTLILNRPGRTREAVGADVASGLADLEAYGQMVLANPDFVTRLKLGAPMNDALPNAFFGGDGRGYIDYPALALTDTVTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LGR7</accession>
    <name>Q1LGR7_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Extra-cytoplasmic solute receptor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">bugT</name>
        <name evidence="4" type="ordered locus">Rmet_3789</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="5">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="5">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Periplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the UPF0065 (bug) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10659.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518307.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3789"/>
    <dbReference type="EnsemblBacteria" id="ABF10659">
        <property type="protein sequence ID" value="ABF10659"/>
        <property type="gene ID" value="Rmet_3789"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3789"/>
    <dbReference type="eggNOG" id="COG3181">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045683_0_1_4"/>
    <dbReference type="OMA" id="VMMFTAD"/>
    <dbReference type="OrthoDB" id="447734at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042597">
        <property type="term" value="C:periplasmic space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005064">
        <property type="entry name" value="BUG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042100">
        <property type="entry name" value="Bug_dom1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42928">
        <property type="entry name" value="PTHR42928"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03401">
        <property type="entry name" value="TctC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017082">
        <property type="entry name" value="YflP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-0675">Receptor</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193161" evidence="3">
        <location>
            <begin position="25"/>
            <end position="326"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF10659.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="326" mass="34409" checksum="7C5BFAE3D4C4E9D9" modified="2006-05-30" version="1">MGLTRKLLGACALTLAFAGTTVHAQDSYPSKPIRFIVPFAPGGVTDLTARTFAKYMGDALKQPLVTENKAGAGATIGANFVAHAAPDGYTMLLGTNVTHAINPRLIAATPYDPLKDFQTVAIFGLNGNVLLVSNSFPAKTFAEFLAYVKARPGRVNYASGSVGSSAHLAAELLKQDVKGLDYTHVPYGGPSEAMSALMGGHVDFLFANIGAAVTQIQSGKVRALAVTTAKRAPQLPDVPTVAESGVPGFEVVGWMAAFVPKGTPAPTIARLNQVINQAQKNPDLRKTLDAAALIPIVESPEQSSRFVREEYEKWGRVIQAANIPPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LGR8</accession>
    <name>Q1LGR8_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Acyl-CoA synthetase (AMP-forming)/AMP-acid ligase II</fullName>
            <ecNumber evidence="3">6.2.1.26</ecNumber>
            <ecNumber evidence="3">6.2.1.3</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">fadD</name>
        <name evidence="3" type="ordered locus">Rmet_3788</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EC" id="6.2.1.26" evidence="3"/>
    <dbReference type="EC" id="6.2.1.3" evidence="3"/>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10658.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518306.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3788"/>
    <dbReference type="EnsemblBacteria" id="ABF10658">
        <property type="protein sequence ID" value="ABF10658"/>
        <property type="gene ID" value="Rmet_3788"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3788"/>
    <dbReference type="eggNOG" id="COG0318">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000022_59_0_4"/>
    <dbReference type="OMA" id="TVPMVYD"/>
    <dbReference type="OrthoDB" id="377638at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004467">
        <property type="term" value="F:long-chain fatty acid-CoA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008756">
        <property type="term" value="F:o-succinylbenzoate-CoA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025110">
        <property type="entry name" value="AMP-bd_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045851">
        <property type="entry name" value="AMP-bd_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020845">
        <property type="entry name" value="AMP-binding_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_Synth/Lig"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13193">
        <property type="entry name" value="AMP-binding_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00455">
        <property type="entry name" value="AMP_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0436">Ligase</keyword>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AMP-binding" evidence="1">
        <location>
            <begin position="25"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="domain" description="AMP-binding_C" evidence="2">
        <location>
            <begin position="429"/>
            <end position="504"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF10658.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="523" mass="56513" checksum="FFBDAA173E84D582" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-10-07" version="45" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LH38</accession>
    <name>Q1LH38_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_3666</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10538.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3666"/>
    <dbReference type="EnsemblBacteria" id="ABF10538">
        <property type="protein sequence ID" value="ABF10538"/>
        <property type="gene ID" value="Rmet_3666"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3666"/>
    <dbReference type="HOGENOM" id="CLU_2384047_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF10538.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="94" mass="10568" checksum="26E58739887DB0A8" modified="2006-05-30" version="1">MEFVSRTLWESGEQGRKLLTFAGIRSKHLHEAAARWLTKQLQDCWTVLDGPPRTERGDIRQDARPATAGDSFACRAPLRPVPVCAPDPSILERK</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LH47</accession>
    <name>Q1LH47_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Type II secretion system protein, pilus assembly, transmembrane part</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">tadB</name>
        <name evidence="8" type="ordered locus">Rmet_3657</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="9">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="9">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10529.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3657"/>
    <dbReference type="EnsemblBacteria" id="ABF10529">
        <property type="protein sequence ID" value="ABF10529"/>
        <property type="gene ID" value="Rmet_3657"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3657"/>
    <dbReference type="eggNOG" id="COG4965">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_065961_0_0_4"/>
    <dbReference type="OMA" id="ETRMSAW"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018076">
        <property type="entry name" value="T2SS_GspF_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00482">
        <property type="entry name" value="T2SSF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0614">Plasmid</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="6"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="85"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="109"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="253"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="283"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="domain" description="T2SSF" evidence="7">
        <location>
            <begin position="142"/>
            <end position="265"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00482"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ABF10529.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="309" mass="33932" checksum="F972E72BA7006A2E" modified="2006-05-30" version="1">MISSASLFIAALAMIMVGLGLLMLAAARSRSRAEDARAHLTAQTEQVRARYATVSDPTPQSATRDLRRRWDDLMRRADLKPTRRTYLVVGVPMVLAVLGGAIAGGGIGAGAMLVMVLTLLCFFVWLRIRRIKEKMLRQLPGFLDGVVRLMTIGSSVPAAFQNSVANTEPPLRQCLVQVIHLQRAGKELDQAVLQMGRAYDLDELVIVSSVLRLSQRYGGRADVVMERTATFMRDREEAQRELMALSAETRMSAWVLGLLPLIVGGGMFVFNAGYILLMWRDPGGRAMLLTAAGLEVAGAFLLYRLARSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LH82</accession>
    <name>Q1LH82_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Major Facilitator Superfamily MFS-1 (Multidrug resistance)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Rmet_3622</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="8">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="8">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10494.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3622"/>
    <dbReference type="EnsemblBacteria" id="ABF10494">
        <property type="protein sequence ID" value="ABF10494"/>
        <property type="gene ID" value="Rmet_3622"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3622"/>
    <dbReference type="eggNOG" id="COG2814">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000960_32_0_4"/>
    <dbReference type="OMA" id="VTHKKWF"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="SSF103473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0614">Plasmid</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="29"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="65"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="95"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="125"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="154"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="185"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="216"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="247"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="277"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="318"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="351"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="389"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="503"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="domain" description="MFS" evidence="6">
        <location>
            <begin position="30"/>
            <end position="525"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABF10494.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="525" mass="57508" checksum="B33C02E17012DB8F" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="96" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LHD4</accession>
    <name>Q1LHD4_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Transcriptional regulator, LysR family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_3570</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the LysR transcriptional regulatory family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10442.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517985.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3570"/>
    <dbReference type="EnsemblBacteria" id="ABF10442">
        <property type="protein sequence ID" value="ABF10442"/>
        <property type="gene ID" value="Rmet_3570"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3570"/>
    <dbReference type="eggNOG" id="COG0583">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039613_16_2_4"/>
    <dbReference type="OMA" id="CYASPEY"/>
    <dbReference type="OrthoDB" id="1661500at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005119">
        <property type="entry name" value="LysR_subst-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000847">
        <property type="entry name" value="Tscrpt_reg_HTH_LysR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00126">
        <property type="entry name" value="HTH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03466">
        <property type="entry name" value="LysR_substrate"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50931">
        <property type="entry name" value="HTH_LYSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH lysR-type" evidence="2">
        <location>
            <begin position="1"/>
            <end position="59"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF10442.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="310" mass="34543" checksum="F9E22BEE63C5BC71" modified="2006-05-30" version="1">MDRLQSMRVFAKLVELGSFARAAQQLEMSNAVVTRYVADLESHLGTRLLNRTTRSLSLTDAGETYLQRCSQILEDVEEAESIVAARSQSLSGTLRLVTPVMFGLHLLPQILERFQQLYPDVVFDVQLSDRMIDIVEEGRDVGVMLSDLGLGSHLVARPLISAEVILCASPGYLREHPPLRNAHDLSNHRAIAMRLPNAEHEWTLSGPEGEVTVPIRPGLLCSNAELAHQAALTNMGIAMLSSYLARPNIEAGRLTHILPQYQLPRRDVSVVYPSRKFLPTKVRAFIDFLLEESQQRAVDTENKPQMTAAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="116" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LHE4</accession>
    <name>Q1LHE4_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Histidine kinase</fullName>
            <ecNumber evidence="2">2.7.13.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">Rmet_3560</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.</text>
            <dbReference type="EC" id="2.7.13.3"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.7.13.3" evidence="2"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10432.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517976.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3560"/>
    <dbReference type="EnsemblBacteria" id="ABF10432">
        <property type="protein sequence ID" value="ABF10432"/>
        <property type="gene ID" value="Rmet_3560"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3560"/>
    <dbReference type="eggNOG" id="COG5000">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019564_0_0_4"/>
    <dbReference type="OMA" id="TELPHNE"/>
    <dbReference type="OrthoDB" id="692375at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00082">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003660">
        <property type="entry name" value="HAMP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003661">
        <property type="entry name" value="HisK_dim/P"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036097">
        <property type="entry name" value="HisK_dim/P_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017232">
        <property type="entry name" value="NtrY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035965">
        <property type="entry name" value="PAS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004358">
        <property type="entry name" value="Sig_transdc_His_kin-like_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00672">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00512">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037532">
        <property type="entry name" value="STHK_NtrY"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00344">
        <property type="entry name" value="BCTRLSENSOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00304">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00388">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47384">
        <property type="entry name" value="SSF47384"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55785">
        <property type="entry name" value="SSF55785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="SSF55874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50885">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 9" id="KW-0418">Kinase</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="13"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="46"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="85"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="365"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="domain" description="HAMP" evidence="8">
        <location>
            <begin position="390"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="7">
        <location>
            <begin position="592"/>
            <end position="827"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ABF10432.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="827" mass="89852" checksum="95B317ECC5992C3E" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="92" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LHG6</accession>
    <name>Q1LHG6_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Histone-like DNA-binding protein (HU)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">hup</name>
        <name evidence="4" type="ordered locus">Rmet_3538</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the bacterial histone-like protein family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10410.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008640984.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3538"/>
    <dbReference type="EnsemblBacteria" id="ABF10410">
        <property type="protein sequence ID" value="ABF10410"/>
        <property type="gene ID" value="Rmet_3538"/>
    </dbReference>
    <dbReference type="GeneID" id="60822643"/>
    <dbReference type="KEGG" id="rme:Rmet_3538"/>
    <dbReference type="eggNOG" id="COG0776">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_105066_3_1_4"/>
    <dbReference type="OMA" id="GAEMQIP"/>
    <dbReference type="OrthoDB" id="1847472at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030527">
        <property type="term" value="F:structural constituent of chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.520.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000119">
        <property type="entry name" value="Hist_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020816">
        <property type="entry name" value="Histone-like_DNA-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010992">
        <property type="entry name" value="IHF-like_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33175">
        <property type="entry name" value="PTHR33175"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00216">
        <property type="entry name" value="Bac_DNA_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01727">
        <property type="entry name" value="DNABINDINGHU"/>
    </dbReference>
    <dbReference type="SMART" id="SM00411">
        <property type="entry name" value="BHL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47729">
        <property type="entry name" value="SSF47729"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00045">
        <property type="entry name" value="HISTONE_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003939"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF10410.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="92" mass="9740" checksum="AE4549EFB539FAF5" modified="2006-05-30" version="1">MTKTELIDAIAAGVDGLTKAKAEQALNVTLGAIMDAVAKGETLSLIGFGTFSKGERGERMARNPRTGEEIKVEAAKTVKFKAGQKFKDAVNQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LHH7</accession>
    <name>Q1LHH7_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Ornithine cyclodeaminase</fullName>
            <ecNumber evidence="1">4.3.1.12</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_3527</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EC" id="4.3.1.12" evidence="1"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10399.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517946.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3527"/>
    <dbReference type="EnsemblBacteria" id="ABF10399">
        <property type="protein sequence ID" value="ABF10399"/>
        <property type="gene ID" value="Rmet_3527"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3527"/>
    <dbReference type="eggNOG" id="COG2423">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_042088_1_2_4"/>
    <dbReference type="OMA" id="QARWQTR"/>
    <dbReference type="OrthoDB" id="1193876at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008473">
        <property type="term" value="F:ornithine cyclodeaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1780.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003462">
        <property type="entry name" value="ODC_Mu_crystall"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023401">
        <property type="entry name" value="ODC_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13812">
        <property type="entry name" value="PTHR13812"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02423">
        <property type="entry name" value="OCD_Mu_crystall"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001439">
        <property type="entry name" value="CryM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0456">Lyase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF10399.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="322" mass="33888" checksum="7E3ED8636DBF8874" modified="2006-05-30" version="1">MTAPAASFVPLDAAATAARLPYPELAQAIAAMLAELRDGTAMAPPRIALPVGDAERGHGTLLVMPARNRDIVMTKNITVHPDNPQRGLPNILGEVVVANAHTGERIALLDGPTVTGRRTAAVSLLAAQSFAPVPDGELLIVGAGVQAQTHLEAFVAGLPVRKVWLHSRTRDKAEALAEHAGRLGVSATVIDDVAEVLPRVSMVVTVTSSRTPVLPDLDSGLWTDRHFIAAVGAFRPEMCELPPRLCHAAAASGRLLADTLFGIEDEAGDLLQAGIDWQTVQPFETAILEADALRARTGSPLVFKSVGYALWDLAACALASKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LHI2</accession>
    <name>Q1LHI2_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Lysophospholipase</fullName>
            <ecNumber evidence="3">3.1.1.5</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">pldB</name>
        <name evidence="3" type="ordered locus">Rmet_3522</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EC" id="3.1.1.5" evidence="3"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10394.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3522"/>
    <dbReference type="ESTHER" id="ralme-q1lhi2">
        <property type="family name" value="Monoglyceridelipase_lysophospholip"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF10394">
        <property type="protein sequence ID" value="ABF10394"/>
        <property type="gene ID" value="Rmet_3522"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3522"/>
    <dbReference type="eggNOG" id="COG2267">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026209_7_2_4"/>
    <dbReference type="OMA" id="FHGFSDH"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004622">
        <property type="term" value="F:lysophospholipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102545">
        <property type="term" value="F:phosphatidyl phospholipase B activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022742">
        <property type="entry name" value="Hydrolase_4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12146">
        <property type="entry name" value="Hydrolase_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00111">
        <property type="entry name" value="ABHYDROLASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="SSF53474"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Hydrolase_4" evidence="2">
        <location>
            <begin position="46"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF10394.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="302" mass="33070" checksum="F89B71CC8F79AF43" modified="2006-05-30" version="1">MTAAGLPGARESAVTGAVPTETRQRMRDGTELLLRTWLPDPQRFAEPLGTVLLVHGLAEHSGRYGHVAAALCALGLRVRAYDHRGHGASGGPRMVAPTPDSYLDDLAEIYDGAMRQWNELPIVLGHSMGGLIAARFATARVRPIRALVLSSPALALRLSNSMLTMHRVLLALMPRIRVPNPIDARLLSHDVEVGRAYRTDPLVQGTISASVLETFIRGMPQALADAPRLEAPMLMLVGGADRIVDPEGSRIFFESAPEDLREMVWFDTGYHEIFNEAEPLRGEVFAALTGWLRQRLETPAKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LHJ1</accession>
    <name>Q1LHJ1_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">ABC-type transporter, periplasmic component</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_3513</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10385.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008650179.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3513"/>
    <dbReference type="EnsemblBacteria" id="ABF10385">
        <property type="protein sequence ID" value="ABF10385"/>
        <property type="gene ID" value="Rmet_3513"/>
    </dbReference>
    <dbReference type="GeneID" id="60822618"/>
    <dbReference type="KEGG" id="rme:Rmet_3513"/>
    <dbReference type="eggNOG" id="COG0683">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027128_0_1_4"/>
    <dbReference type="OMA" id="PVSHGIM"/>
    <dbReference type="OrthoDB" id="776281at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028081">
        <property type="entry name" value="Leu-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028082">
        <property type="entry name" value="Peripla_BP_I"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13458">
        <property type="entry name" value="Peripla_BP_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53822">
        <property type="entry name" value="SSF53822"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193503" evidence="1">
        <location>
            <begin position="24"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="domain" description="Peripla_BP_6" evidence="2">
        <location>
            <begin position="24"/>
            <end position="375"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF10385.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="382" mass="40867" checksum="05D712568850B407" modified="2006-05-30" version="1">MTFKRASLLALAAASLFAGAANAQVKVGVTVSATGPAASLGIPEKNTFTLLPKEIAGKKIEYIVLDDATDTTTAVKNTRKLISEDKVDVVVGSTVTPNSLAMVDVAAESETPMISMAASARIIEPMDAKRAWIFKTPQNDSHMATAIAEHMTNNNVKTVAFIGFADAYGDSWGQEFAKVAEMRKIKVVANERFARTDNSVTGQVLKMMGANPDAVLIAGSGTPAALPEKALKERGYKGKIYQTHGVANPDFLRVCGKDCEGTFLPAGPLLVAEQLPDSNPVKKPALTYKTAYEKAFGGQVSTFGGHAWDAGLLLQNAIPEALKKGQPGTKEFRKALRDALEKTTNLPVSHGIINMTPTDHLGMDQRARVMVQIVDGKWKLQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LHK3</accession>
    <name>Q1LHK3_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Membrane-bound ATP synthase subunit, F1-F0-type proton-ATPase</fullName>
            <ecNumber evidence="8">3.6.3.14</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">atpI</name>
        <name evidence="8" type="ordered locus">Rmet_3501</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="3.6.3.14" evidence="8"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10373.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3501"/>
    <dbReference type="EnsemblBacteria" id="ABF10373">
        <property type="protein sequence ID" value="ABF10373"/>
        <property type="gene ID" value="Rmet_3501"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3501"/>
    <dbReference type="eggNOG" id="COG3312">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_121415_0_0_4"/>
    <dbReference type="OMA" id="GAICWVP"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005598">
        <property type="entry name" value="ATP_synth_I"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03899">
        <property type="entry name" value="ATP-synt_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="72"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="98"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="132"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="158"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ABF10373.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="179" mass="20043" checksum="092C9C2D0F5F3A09" modified="2006-05-30" version="1">MVRDRQQDRSQAGKASGNDRRDERQQDVWRDDDWDDSAEREEVAVDPLSHAEAVKLLGERALRPSRMTPGKVVLAQVAVTLLSALAWAIFAHEHAPSGWSALFGGMVCVVPSGFFALRLWMSRRQPTVGGLVAGEAIKVFSTVALFVLVVVLYRDLRWIPMLVTFLLVLKTYWVALAIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LHN8</accession>
    <name>Q1LHN8_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Major facilitator superfamily (MFS) transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Rmet_3466</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10338.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517896.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3466"/>
    <dbReference type="EnsemblBacteria" id="ABF10338">
        <property type="protein sequence ID" value="ABF10338"/>
        <property type="gene ID" value="Rmet_3466"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3466"/>
    <dbReference type="eggNOG" id="COG2814">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047644_2_0_4"/>
    <dbReference type="OMA" id="GWNFMFV"/>
    <dbReference type="OrthoDB" id="1121832at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="SSF103473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="48"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="73"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="98"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="135"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="167"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="229"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="281"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="305"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="366"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="domain" description="MFS" evidence="6">
        <location>
            <begin position="208"/>
            <end position="402"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABF10338.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="402" mass="41706" checksum="D8A38673D0AE2B57" modified="2006-05-30" version="1">MASHALRQARVLALCQALYTSALSVDLTLTGLVGYTLAPNKALATLPFALITVASALTAIAAAFLIERLGRRPAFCLGGLACMAGGLVSVLAIWRHDFVLFCLGTALVGVFQAFARFYRLAAADAAPLAEKPRAISAVLTGGVVAAVCGPAIAAWSADMMQPLPFAGSYAVVAVFGALSVAVLALAYREPPATAHAAAAVDNLPARPLGEIFRQPVFLASFANNGIGNAVMMFMMTATPIAAVACQHTIKQGAQIIEWHLVGMYAPSFFSGWLLQRFGNPVMLVSGIALSALAAIVALCSTTLPAFYLALLCLGVGWNFMFVGGTTLLTSSYRPAERARTQAASEFSAAALTALATLAAGQVLNQWGWQGVNLAVLPALAVALAVTLAWQRQERLAPSAAGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="104" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LHQ2</accession>
    <name>Q1LHQ2_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 11">Pyruvate kinase</fullName>
            <ecNumber evidence="3 11">2.7.1.40</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">pyk</name>
        <name evidence="14" type="ordered locus">Rmet_3452</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>ATP + pyruvate = ADP + H(+) + phosphoenolpyruvate</text>
            <dbReference type="Rhea" id="RHEA:18157"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.40"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 11">Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 5/5.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the pyruvate kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.40" evidence="3 11"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10324.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008646154.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3452"/>
    <dbReference type="EnsemblBacteria" id="ABF10324">
        <property type="protein sequence ID" value="ABF10324"/>
        <property type="gene ID" value="Rmet_3452"/>
    </dbReference>
    <dbReference type="GeneID" id="60822557"/>
    <dbReference type="KEGG" id="rme:Rmet_3452"/>
    <dbReference type="eggNOG" id="COG0469">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015439_8_2_4"/>
    <dbReference type="OMA" id="QVPIVQK"/>
    <dbReference type="OrthoDB" id="1098983at2"/>
    <dbReference type="UniPathway" id="UPA00109">
        <property type="reaction ID" value="UER00188"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030955">
        <property type="term" value="F:potassium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004743">
        <property type="term" value="F:pyruvate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.33.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1380.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001697">
        <property type="entry name" value="Pyr_Knase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015813">
        <property type="entry name" value="Pyrv/PenolPyrv_Kinase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040442">
        <property type="entry name" value="Pyrv_Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011037">
        <property type="entry name" value="Pyrv_Knase-like_insert_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015793">
        <property type="entry name" value="Pyrv_Knase_brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015795">
        <property type="entry name" value="Pyrv_Knase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036918">
        <property type="entry name" value="Pyrv_Knase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015806">
        <property type="entry name" value="Pyrv_Knase_insert_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11817">
        <property type="entry name" value="PTHR11817"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00224">
        <property type="entry name" value="PK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02887">
        <property type="entry name" value="PK_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01050">
        <property type="entry name" value="PYRUVTKNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50800">
        <property type="entry name" value="SSF50800"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51621">
        <property type="entry name" value="SSF51621"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52935">
        <property type="entry name" value="SSF52935"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01064">
        <property type="entry name" value="pyruv_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9 11" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="6 11 14" id="KW-0418">Kinase</keyword>
    <keyword evidence="8 11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10 14" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11 14" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PK" evidence="12">
        <location>
            <begin position="5"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="domain" description="PK_C" evidence="13">
        <location>
            <begin position="355"/>
            <end position="467"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004997"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF02887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ABF10324.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="488" mass="51797" checksum="C62D4B75235A7E35" modified="2006-05-30" version="1">MRRQRKAKIVATLGPASTDIAVIRQLFDAGADVFRLNFSHGTHEDHRARYDAVRQVEAETGRPIAILADLQGPKLRIGTFAAGKVAVSAGDTFVLDSDPTPGDAKRVYLPHPELFKAANAGQSLLIDDGKVRLNIESVASGSITTRVANNGTLSDRKGVNVPDAVIPIPALTEKDRKDLAFALELGADWIALSFVQRPSDILEARELIGTRAGVLSKIEKPAALQQLEEIVRVSDAVMVARGDLGVELPPERVPGVQKRILRICRQLGKPIVIATQMLESMIDSPVPTRAEASDVASAIYDGADAVMLSAESANGRHPVAAVSIMNRIITEVENDPLYRNMIDAQHEAPLSTRQDAICAALREVTQIIGAKATVTYTSSGATALRAARERPCAPIVSITPNLEIARRLAITWGVHSTVSPDVQSVDEMVEAAARAALVEGYAAPGDQITIAAGMPFGQGGTTNLLRVAEVGTEVAGLDIGKVPAAEAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LHR0</accession>
    <name>Q1LHR0_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">C4-dicarboxylic acid, orotate and citrate transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">dctA2</name>
        <name evidence="8" type="ordered locus">Rmet_3444</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10316.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517876.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3444"/>
    <dbReference type="EnsemblBacteria" id="ABF10316">
        <property type="protein sequence ID" value="ABF10316"/>
        <property type="gene ID" value="Rmet_3444"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3444"/>
    <dbReference type="eggNOG" id="COG1301">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019375_7_0_4"/>
    <dbReference type="OMA" id="GHGMNVD"/>
    <dbReference type="OrthoDB" id="781228at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015293">
        <property type="term" value="F:symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046942">
        <property type="term" value="P:carboxylic acid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3860.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001991">
        <property type="entry name" value="Na-dicarboxylate_symporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018107">
        <property type="entry name" value="Na-dicarboxylate_symporter_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036458">
        <property type="entry name" value="Na:dicarbo_symporter_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42865">
        <property type="entry name" value="PTHR42865"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00375">
        <property type="entry name" value="SDF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF118215">
        <property type="entry name" value="SSF118215"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00713">
        <property type="entry name" value="NA_DICARBOXYL_SYMP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00714">
        <property type="entry name" value="NA_DICARBOXYL_SYMP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0769">Symport</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="23"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="61"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="91"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="167"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="208"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="236"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="306"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="339"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="368"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="394"/>
            <end position="418"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ABF10316.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="467" mass="48861" checksum="D24DA8850CE000A5" modified="2006-05-30" version="1">MQQAVPSQRAAGARLHTRFTRSLFGQVLIALVIGTLLGLFFPEFAAKLKPLGDAFIKLIKMLIGPIVFCVVVAGICGAGELKKVGRVGIKAVVYFEVVTTIALALGVVLAYVFHPGTGMNVNPRSLDISAMSAYIESADKVKSAGMVDFLLKLIPNTIMGAFSSGDVLQVLLVSILFGCALSVVGESGRPLVKLIDTFSHTLFKMMGFIIKLAPLGVLGAVAFTVGKYGIGSLKQLGFLVILFYGAVVIFVVGVLGLIMRLCGFSVLKLIRYLRAELLVVLGTASSDSVLPQVMKKLEYMGIRKSVVGLVIPTGYSFNLDAFSIYLTLAAVFIAQATNTPLALGDLLGILAVALVTSKGAHGIPGSAIVILAATLSAHPAIPAIGLVLVLSVDWFIGIARAVGNLIGNCVATVVVAAWEHDIDRERAHGVLNGTIPAGELEEGLMVHDHGSGEVPVPAPLPGVASGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LHR7</accession>
    <name>Q1LHR7_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Phosphinothricin N-acetyltransferase</fullName>
            <ecNumber evidence="3">2.3.1.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">pat</name>
        <name evidence="3" type="ordered locus">Rmet_3437</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.3.1.-" evidence="3"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10309.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3437"/>
    <dbReference type="EnsemblBacteria" id="ABF10309">
        <property type="protein sequence ID" value="ABF10309"/>
        <property type="gene ID" value="Rmet_3437"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3437"/>
    <dbReference type="eggNOG" id="COG1247">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013985_4_2_4"/>
    <dbReference type="OMA" id="EAAGIWT"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008080">
        <property type="term" value="F:N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="SSF55729"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="2">
        <location>
            <begin position="32"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF10309.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="206" mass="22661" checksum="E91F3AE054FCC4BF" modified="2006-05-30" version="1">MASDDVPWQIDPSESPSESNVSALPTIARKPYRLRDATTDDVPAIHAIYAHHVQHGRASFEEVAPTVDEMRHRFGEVLKKGLPYIVAERDGEILGYAYASSYRARSAYRFAIEDSIYIDHRHTGEGLGRALLAELITRCETGPWRQMVAVVALTASGEGAGSVAVHERLGFRTVGRLESVGLKHGQWIDTLLMQRPLGVGDATIPD</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="70" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LHS6</accession>
    <name>Q1LHS6_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_3428</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10300.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517862.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3428"/>
    <dbReference type="EnsemblBacteria" id="ABF10300">
        <property type="protein sequence ID" value="ABF10300"/>
        <property type="gene ID" value="Rmet_3428"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3428"/>
    <dbReference type="eggNOG" id="COG2823">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_083606_1_0_4"/>
    <dbReference type="OMA" id="RVKKVHN"/>
    <dbReference type="OrthoDB" id="1363906at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007055">
        <property type="entry name" value="BON_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014004">
        <property type="entry name" value="Transpt-assoc_nodulatn_dom_bac"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04972">
        <property type="entry name" value="BON"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00749">
        <property type="entry name" value="BON"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50914">
        <property type="entry name" value="BON"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BON" evidence="2">
        <location>
            <begin position="63"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="domain" description="BON" evidence="2">
        <location>
            <begin position="140"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="217"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="217"/>
            <end position="270"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF10300.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="284" mass="29258" checksum="A8BCB4421B732AFC" modified="2006-05-30" version="1">MTNVTKTNQKKTSPARIARTAVTVAALIVSATQLAGCFPVLAGAVGTGVVMATDRRPTGTQTIDRGLQMEIDSTLSSRFSGDKARVEAAVFNRKVLLVGEVSNADVKQQVEQYVRGLQNTRDVVNELQVTASPGFMTRSNDAYLTSKVKTLMVTAEGVPANSIKVTTDKGVVYLLGIVTNAEGDKATDVARNSSGVQKVVKAFDYISESERARLDALGSSQNGNTGSEAVGTPAPVQSVPGSAPNAPVNSDAPASMGTSGGATTSPVAAPVSSPVALPPGRNLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LI35</accession>
    <name>Q1LI35_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative dehydrogenase/oxygenase subunit (Flavoprotein)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_3319</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10191.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517770.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3319"/>
    <dbReference type="EnsemblBacteria" id="ABF10191">
        <property type="protein sequence ID" value="ABF10191"/>
        <property type="gene ID" value="Rmet_3319"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3319"/>
    <dbReference type="eggNOG" id="COG0654">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033694_0_0_4"/>
    <dbReference type="OMA" id="GYEVTLM"/>
    <dbReference type="OrthoDB" id="1077270at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041654">
        <property type="entry name" value="StyA_sbd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17885">
        <property type="entry name" value="Smoa_sbd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="SSF51905"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Smoa_sbd" evidence="1">
        <location>
            <begin position="149"/>
            <end position="256"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF17885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF10191.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="413" mass="45343" checksum="07C9E897DEF683EF" modified="2006-05-30" version="1">MSQRIAIVGAGQSGLQMALGLQARGYRVTLLSNRTADQIRAGKVMSSQCMFDRALQTERDLDLNWWEEACPPVEGIGLAIPHPERRGEKLIDWAAPLDRVAQAVDQRLKMPAWMDAFVERGGDLRIVDVGLNELEALTLDHDLVLLAAGKGEIVSRFERDAARSPFSAPQRALALTYVTGMLPRTPFSRVCFNLIPGVGEYFVFPALTLSGPCEIMVFEGVPGGDMDRWSEARTPEAHLEESLRILRTYLPWEAERCDRVALTDANGILSGRFAPTVRKPVLTLPSGRLVFGMADAVVVNDPITGQGSNNAAKCCKVYLDAILARDGLPYDADWMNQTFERYWAYAGDVVNWTNSLLTPPPPHILGLLGAAGQSPALAARIANGFDNPPDFFPWWSDPLACQQLIDQQLAKAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="69" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LI72</accession>
    <name>Q1LI72_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="9">ABC-type transport system involved in cytochrome c biogenesis, permease component</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">Rmet_3282</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10154.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3282"/>
    <dbReference type="EnsemblBacteria" id="ABF10154">
        <property type="protein sequence ID" value="ABF10154"/>
        <property type="gene ID" value="Rmet_3282"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3282"/>
    <dbReference type="eggNOG" id="COG0755">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_049710_2_1_4"/>
    <dbReference type="OMA" id="MWYSVAR"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017004">
        <property type="term" value="P:cytochrome complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002541">
        <property type="entry name" value="Cyt_c_assembly"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017562">
        <property type="entry name" value="Cyt_c_biogenesis_CcsA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045062">
        <property type="entry name" value="Cyt_c_biogenesis_CcsA/CcmC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30071">
        <property type="entry name" value="PTHR30071"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01578">
        <property type="entry name" value="Cytochrom_C_asm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03144">
        <property type="entry name" value="cytochr_II_ccsB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0201">Cytochrome c-type biogenesis</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="48"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="96"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="134"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="167"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="196"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="231"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="275"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="314"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="348"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="377"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="domain" description="Cytochrom_C_asm" evidence="8">
        <location>
            <begin position="198"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01578"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ABF10154.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="407" mass="45593" checksum="9E8D97E700BFC989" modified="2006-05-30" version="1">MSSNVNHVNPGLTPRRAAATDDVSRDAAGQDTAYLEPSFLSRLGWTDWVFALLLVVGAGFALSRYGQWMNVYDKGVLIAAVPTFALLGWHWKPVRLLMIGIAAFSLFAIQLYQGDLARAEQAFFLKYFLSSQSAILWMSALVFLSTLFYWVGLISRSETGYSIGSKLCWAAVVLGFTGLMVRWYESYLIGADIGHIPISNLYEVFVLFSLITSLFYLYYQRRYATRALGPFVMLVVSAAVGFLLWYTVSRNAQEIQPLVPALQSWWMKIHVPANFIGYGTFALAAMVSVAYLLKQRGILAERLPSLELLDDVMYKAIAVGFAFFTIATILGALWAAEAWGGYWSWDPKETWALIVWLNYAAWLHMRLMKGLRGTVAAWWALVGLLVTTFAFLGVNMFLSGLHSYGEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="105" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LIA7</accession>
    <name>Q1LIA7_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Histidinol-phosphate aminotransferase</fullName>
            <ecNumber evidence="6">2.6.1.9</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Imidazole acetol-phosphate transaminase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6 8" type="primary">hisC</name>
        <name evidence="8" type="ordered locus">Rmet_3247</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>2-oxoglutarate + L-histidinol phosphate = 3-(imidazol-4-yl)-2-oxopropyl phosphate + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:23744"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:57766"/>
            <dbReference type="ChEBI" id="CHEBI:57980"/>
            <dbReference type="EC" id="2.6.1.9"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 6">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3 6">Amino-acid biosynthesis; L-histidine biosynthesis; L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 7/9.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4 6">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the class-II pyridoxal-phosphate-dependent aminotransferase family. Histidinol-phosphate aminotransferase subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.6.1.9" evidence="6"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10119.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517722.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3247"/>
    <dbReference type="EnsemblBacteria" id="ABF10119">
        <property type="protein sequence ID" value="ABF10119"/>
        <property type="gene ID" value="Rmet_3247"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3247"/>
    <dbReference type="eggNOG" id="COG0079">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017584_3_1_4"/>
    <dbReference type="OMA" id="IWLNANE"/>
    <dbReference type="OrthoDB" id="1248286at2"/>
    <dbReference type="UniPathway" id="UPA00031">
        <property type="reaction ID" value="UER00012"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004400">
        <property type="term" value="F:histidinol-phosphate transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000105">
        <property type="term" value="P:histidine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01023">
        <property type="entry name" value="HisC_aminotrans_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005861">
        <property type="entry name" value="HisP_aminotrans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="SSF53383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01141">
        <property type="entry name" value="hisC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="5 6 8" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="6" id="KW-0368">Histidine biosynthesis</keyword>
    <keyword evidence="6" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aminotran_1_2" evidence="7">
        <location>
            <begin position="43"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="6">
        <location>
            <position position="226"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001045"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ABF10119.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="366" mass="39667" checksum="8E91AD34E4BE69FB" modified="2006-05-30" version="1">MSAVDPSLIERIIRDDVRAMGAYHVADSHGLVKLDAMENPYRLPPELRAQLAARLGEVALNRYPVPSSEALRAKLKTVMNVPAGMDVLLGNGSDEIISMLSLAAARPGAKVMAPVPGFVMYAMSAQFAGLEFVGVPLRPDFTLDRAAMLAAMAEHKPAIIYLAYPNNPTGNLFDADDMAAIVRAAQGEVCQSLVVVDEAYQPFAQESWMPRLTEFGNLLVMRTVSKLGLAGIRLGYVAGAPEWLSQLDKVRPPYNVNVLTEATALFALEHVAVLDDQAAKLRAERSKLVEAMAGMPGVTVFPSAANFVLVRVPNAAQIFDRLLARKVLIKNVSKMHTLLANCLRVTVSTPEENAQFLEAFKASLQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="98" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LIE5</accession>
    <name>Q1LIE5_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Transcriptional regulator, AsnC/Lrp-family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Rmet_3209</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10081.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008643127.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3209"/>
    <dbReference type="EnsemblBacteria" id="ABF10081">
        <property type="protein sequence ID" value="ABF10081"/>
        <property type="gene ID" value="Rmet_3209"/>
    </dbReference>
    <dbReference type="GeneID" id="60825502"/>
    <dbReference type="KEGG" id="rme:Rmet_3209"/>
    <dbReference type="eggNOG" id="COG1522">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_091233_5_3_4"/>
    <dbReference type="OMA" id="NIFCKIR"/>
    <dbReference type="OrthoDB" id="1844486at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000485">
        <property type="entry name" value="AsnC-type_HTH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011008">
        <property type="entry name" value="Dimeric_a/b-barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019888">
        <property type="entry name" value="Tscrpt_reg_AsnC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019887">
        <property type="entry name" value="Tscrpt_reg_AsnC/Lrp_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01037">
        <property type="entry name" value="AsnC_trans_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13404">
        <property type="entry name" value="HTH_AsnC-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00033">
        <property type="entry name" value="HTHASNC"/>
    </dbReference>
    <dbReference type="SMART" id="SM00344">
        <property type="entry name" value="HTH_ASNC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54909">
        <property type="entry name" value="SSF54909"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50956">
        <property type="entry name" value="HTH_ASNC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH asnC-type" evidence="4">
        <location>
            <begin position="4"/>
            <end position="73"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50956"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABF10081.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="149" mass="16197" checksum="9E6734D1A3878D71" modified="2006-05-30" version="1">MAELDQTDRHLLALLQANARESAANLARALGIARTTVVARIARLEKIGVIAGYGVRLGRQMEDNAILAFCGLSVQPKASPAVVRALQRFPEIEELNSVSGPVDYMAVIRCDSHARLDKLLDEIGMLDGVNHTQTSIVLARKIDRRRAAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="61" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LIW2</accession>
    <name>Q1LIW2_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">WYL domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_3042</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09914.1"/>
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    </dbReference>
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    </dbReference>
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    <dbReference type="EnsemblBacteria" id="ABF09914">
        <property type="protein sequence ID" value="ABF09914"/>
        <property type="gene ID" value="Rmet_3042"/>
    </dbReference>
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    <dbReference type="HOGENOM" id="CLU_054168_2_0_4"/>
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    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="WYL_dom_s026"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="135"/>
            <end position="208"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF09914.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="300" mass="34227" checksum="75557D4953C4A674" modified="2006-05-30" version="1">MQKPSVPAAEPAEPKGLSWGLESRLQFIDFRLRWERRINRMDLTEHFGISVPQASLDISKYMELAPSNLTYDRSSKTYTAAQSFHPLYQRSSAQRYLAELLATKMGIVEPTASFIGSAPETDWAPSPWRTINEQTVEAVVRAIRQQESILVSYQSMTSLDESVRLLSPHALGNDGFRWHMRAFCHKRQRFSDFVLARILRIDGFEATQVDANQDSHWQTVLTLVLAPHPDLPAAKKRVLELDYGMEDGEVKLPCRQAFLYYTLRRLGLHTKEAPDPLAQQITLKNRDEIQPYIDALAAQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LJ37</accession>
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        <submittedName>
            <fullName evidence="3">Response regulator containing an atypical phosphorylation pocket</fullName>
        </submittedName>
    </protein>
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        <name evidence="3" type="ordered locus">Rmet_2966</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
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        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
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                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
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    </dbReference>
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    <dbReference type="EnsemblBacteria" id="ABF09839">
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
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    </dbReference>
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    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Response regulatory" evidence="2">
        <location>
            <begin position="18"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="1">
        <location>
            <position position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF09839.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="349" mass="39865" checksum="F760FE55FEB681AA" modified="2006-05-30" version="1">MTFSSHDRNLSVFHHPVLTVLVDDSKSFIDSLAFQMDASRAVVSFTDPREALAWIREAYATRHPAFLPVHVTHDDLTFLSERRTVQLDVDRIHRQIHDVKRFLQPGVIVVDYSMPQMDGLEFCQALEGLPCKTILLTGTADESIAVQGFNHGLIDRYVKKHDPNMVARLNEEIDALQQQYFASISRTLRDLLTRHSYSFLSDPAMADRVRQTAARYGFVEYYLYPNPVGMLLLTAQGHATLMVIETRAGLMSQVESAEAYEAPAALIEGLREGRIVPFFWPGNGMYTPACVDWEQYCLPAERCEGHEEYFYALFDLPRHMLQEPVVSLQSFLAEFTRNPELMTGRNKRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-10-07" version="57" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LJZ2</accession>
    <name>Q1LJZ2_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_2661</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
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            <taxon>Betaproteobacteria</taxon>
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        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09534.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2661"/>
    <dbReference type="EnsemblBacteria" id="ABF09534">
        <property type="protein sequence ID" value="ABF09534"/>
        <property type="gene ID" value="Rmet_2661"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2661"/>
    <dbReference type="HOGENOM" id="CLU_2540126_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF09534.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="83" mass="9367" checksum="B8BEC5A8ED2C86F0" modified="2006-05-30" version="1">MFWRCQRSANGEARGLVRRTVRAPFAANMVVIKATSWDLGSVLILASIVRRYRVDADPTHVPKVVGRVTVRSGNGVRVRLHRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LK30</accession>
    <name>Q1LK30_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Permease of the drug/metabolite transporter (DMT) superfamily</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_2619</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
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        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09496.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008649840.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2619"/>
    <dbReference type="EnsemblBacteria" id="ABF09496">
        <property type="protein sequence ID" value="ABF09496"/>
        <property type="gene ID" value="Rmet_2619"/>
    </dbReference>
    <dbReference type="GeneID" id="60820945"/>
    <dbReference type="KEGG" id="rme:Rmet_2619"/>
    <dbReference type="eggNOG" id="COG0697">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032828_0_1_4"/>
    <dbReference type="OMA" id="MGVCVKL"/>
    <dbReference type="OrthoDB" id="1420919at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000620">
        <property type="entry name" value="EamA_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00892">
        <property type="entry name" value="EamA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="33"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="67"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="94"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="121"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="146"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="177"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="202"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="232"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="261"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="domain" description="EamA" evidence="2">
        <location>
            <begin position="147"/>
            <end position="273"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF09496.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="306" mass="32692" checksum="9E3CC1F49E63F2F5" modified="2006-05-30" version="1">MQSLWMLFAAFSFSLMGVGVKLAAEFYTTGEIVFYRSLIGVTIMWIMLASRGVPVSTPHMATHIKRSVFGVTALLLWFTSISMLPLATAMTLNYMSPVWIALILGASAALAGTAGAADRKLIGAILLSFAGVICLLQPSVGQDQLTGGLIGLISGVFTALAYVEVRQLGQLGEPEGRIVFYFSAVGLVCGLVWMLFTGVSPHTWHSAGLLLAIGILATLGQTSMTRAYKRGNTLLTANLQYAGIVFSSLWGILVWSDKLNWISWLGMALIVASGIATTLTRARATDKHPTPATPVKSLEAEVHPEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LK53</accession>
    <name>Q1LK53_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Extra-cytoplasmic Solute Receptor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">bug</name>
        <name evidence="4" type="ordered locus">Rmet_2596</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Periplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the UPF0065 (bug) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09473.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517176.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2596"/>
    <dbReference type="EnsemblBacteria" id="ABF09473">
        <property type="protein sequence ID" value="ABF09473"/>
        <property type="gene ID" value="Rmet_2596"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2596"/>
    <dbReference type="eggNOG" id="COG3181">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045683_0_0_4"/>
    <dbReference type="OMA" id="ETSTIMV"/>
    <dbReference type="OrthoDB" id="447734at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042597">
        <property type="term" value="C:periplasmic space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005064">
        <property type="entry name" value="BUG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042100">
        <property type="entry name" value="Bug_dom1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42928">
        <property type="entry name" value="PTHR42928"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03401">
        <property type="entry name" value="TctC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017082">
        <property type="entry name" value="YflP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0675">Receptor</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193571" evidence="3">
        <location>
            <begin position="26"/>
            <end position="327"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF09473.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="327" mass="34916" checksum="CF1084BEFAD63FA0" modified="2006-05-30" version="1">MNRSFRVRLRPLLATLALAPALSFAQDKFPSRPITWIVPFTPGGITDTASRTIAKKMSETLKQSVVVENRPGAGGMVGTEVAARAPADGYTWIYGTQGTMAANLALYSKVRYDPLKDFAPVHALSETSTIMVVNASRPYKTVKELVEYAKQHPGKVNFGSAGAGTGTHLSAELFQASAGIKLTHIPYKGSSPAINDLLGGTVDVMFDYPSIVLPFIQSGKLRALATTGKDRLELLPNVPTIAESGFAMAQASSWSGILVPAGTPPQTVALISKATEEALRDPEVRKSIEPYGSMPMYGLSEAKFGSFIQAEIVKWGDVVKKSGAKLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LK60</accession>
    <name>Q1LK60_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">Rmet_2589</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the four-carbon acid sugar kinase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09466.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517168.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2589"/>
    <dbReference type="EnsemblBacteria" id="ABF09466">
        <property type="protein sequence ID" value="ABF09466"/>
        <property type="gene ID" value="Rmet_2589"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2589"/>
    <dbReference type="eggNOG" id="COG3395">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029424_0_1_4"/>
    <dbReference type="OMA" id="IASCVPW"/>
    <dbReference type="OrthoDB" id="771666at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10840">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.980.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037051">
        <property type="entry name" value="4-carb_acid_sugar_kinase_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010737">
        <property type="entry name" value="DUF1537"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031475">
        <property type="entry name" value="NBD_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042213">
        <property type="entry name" value="NBD_C_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17042">
        <property type="entry name" value="NBD_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07005">
        <property type="entry name" value="SBD_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="SBD_N" evidence="7">
        <location>
            <begin position="4"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="domain" description="NBD_C" evidence="8">
        <location>
            <begin position="245"/>
            <end position="400"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005715"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF07005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF17042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ABF09466.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="410" mass="42740" checksum="A44C069CF94B1CF9" modified="2006-05-30" version="1">MTWLIIADDLSGAADCAIGFAAHGARAVVTLDVADSVGIADAEIVAADVDSRRMTPEDATTSNIDAWHRGDGRHRRLYKKIDSTLRGNWAAETVALQPLAGLAIIAPAFPATGRTTRGGRMFVNGQPLEDTDIWRLEGLNGHADMVALLAARGLATALLPLDVVRAGAPAVLAELRARQADGIAAVVCDAETEQDLVTLADASNQLDTPAYWVGSGGLARSLSTVAQVTRAETTSPVTYHQGPVLTMVGSMSGISGKQAAYLCERARMEALVVAPQTLRDGPGHPAWTTTRQSITNCLSAGRDLLLSIGRDDAFDPAEGPRLSTALAQLALPSFEHVGGLIATGGETARAMLGAAGIAALALRHEVEPGVPLSDTIESLPGAPARRVATKAGAFGTEAALWLAWQAMRQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="67" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LKC1</accession>
    <name>Q1LKC1_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">MaoC_dehydrat_N domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_2528</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09405.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517104.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2528"/>
    <dbReference type="EnsemblBacteria" id="ABF09405">
        <property type="protein sequence ID" value="ABF09405"/>
        <property type="gene ID" value="Rmet_2528"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2528"/>
    <dbReference type="eggNOG" id="COG2030">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_116276_1_1_4"/>
    <dbReference type="OMA" id="HAPVCAG"/>
    <dbReference type="OrthoDB" id="1380653at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029069">
        <property type="entry name" value="HotDog_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039569">
        <property type="entry name" value="MaoC-like_dehydrat_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016709">
        <property type="entry name" value="UPF0336"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13452">
        <property type="entry name" value="MaoC_dehydrat_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF018072">
        <property type="entry name" value="UCP018072"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54637">
        <property type="entry name" value="SSF54637"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MaoC_dehydrat_N" evidence="1">
        <location>
            <begin position="5"/>
            <end position="140"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF09405.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="149" mass="16383" checksum="C8EC642D560E8105" modified="2006-05-30" version="1">MADKSMIGMDLGKGSIDVEKGRLRFFAKATGETSPEYVDESAARDAGHRSLPVPPTFFMCLNSDVAAASGIDRMKVLQLDLGRILHAEQAFDYHRMAYAGDVLQFETTLTDVYDKKGGALHFVVTTTRVTNQNGEHVADMRGTLVERRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="56" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LKK5</accession>
    <name>Q1LKK5_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_2444</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09321.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008648396.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2444"/>
    <dbReference type="DNASU" id="4039266"/>
    <dbReference type="EnsemblBacteria" id="ABF09321">
        <property type="protein sequence ID" value="ABF09321"/>
        <property type="gene ID" value="Rmet_2444"/>
    </dbReference>
    <dbReference type="GeneID" id="60821082"/>
    <dbReference type="KEGG" id="rme:Rmet_2444"/>
    <dbReference type="eggNOG" id="ENOG5032SQZ">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2383451_0_0_4"/>
    <dbReference type="OMA" id="LWIACKV"/>
    <dbReference type="OrthoDB" id="1807616at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="16"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="46"/>
            <end position="67"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF09321.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="93" mass="10353" checksum="F10D9EAEC0EB298F" modified="2006-05-30" version="1">MNQEIAYLHPVKTARALVLVYLCFSVPIVAMGLFVAFIRYGDLPGITVFTALILNALIGFGLLWLGCKAYNWVAARFGGIEVHVRELTPEDRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="61" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LKV2</accession>
    <name>Q1LKV2_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_2347</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09224.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_003153634.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2347"/>
    <dbReference type="EnsemblBacteria" id="ABF09224">
        <property type="protein sequence ID" value="ABF09224"/>
        <property type="gene ID" value="Rmet_2347"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2347"/>
    <dbReference type="eggNOG" id="ENOG502ZUPX">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_148565_1_0_4"/>
    <dbReference type="OMA" id="VAWHSLT"/>
    <dbReference type="OrthoDB" id="1923518at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021356">
        <property type="entry name" value="Integr_conj_element_PFL4702"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11190">
        <property type="entry name" value="DUF2976"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03745">
        <property type="entry name" value="conj_TIGR03745"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193181" evidence="2">
        <location>
            <begin position="30"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="53"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="90"/>
            <end position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF09224.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="119" mass="12733" checksum="A43F5E8C990526F6" modified="2006-05-30" version="1">MHNRILTSRLAQRAAMALGAAALPALSFAQGLPQLENPTRGTGNGIMETIRNYGYDIIMLVALLVVASMFIGVCYHAYGTYAEIHTGRKTWGQFGLTVAIGAVLLVIGIWLLTEATGIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="53" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LLC6</accession>
    <name>Q1LLC6_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_2171</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09050.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2171"/>
    <dbReference type="EnsemblBacteria" id="ABF09050">
        <property type="protein sequence ID" value="ABF09050"/>
        <property type="gene ID" value="Rmet_2171"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2171"/>
    <dbReference type="eggNOG" id="ENOG5033V0T">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_299730_0_0_4"/>
    <dbReference type="OMA" id="LLMINDG"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF09050.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="1000" mass="113072" checksum="8DBDA62ED62D9705" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LLJ8</accession>
    <name>Q1LLJ8_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Protein HflK</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">hflK</name>
        <name evidence="8" type="ordered locus">Rmet_2099</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">HflC and HflK could encode or regulate a protease.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">HflC and HflK may interact to form a multimeric complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the band 7/mec-2 family. HflK subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08978.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516812.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2099"/>
    <dbReference type="EnsemblBacteria" id="ABF08978">
        <property type="protein sequence ID" value="ABF08978"/>
        <property type="gene ID" value="Rmet_2099"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2099"/>
    <dbReference type="eggNOG" id="COG0330">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039173_2_0_4"/>
    <dbReference type="OMA" id="WNEPGGN"/>
    <dbReference type="OrthoDB" id="577287at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008233">
        <property type="term" value="F:peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03404">
        <property type="entry name" value="SPFH_HflK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.479.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001107">
        <property type="entry name" value="Band_7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036013">
        <property type="entry name" value="Band_7/SPFH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010201">
        <property type="entry name" value="HflK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020980">
        <property type="entry name" value="Membrane_HflK_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43327:SF2">
        <property type="entry name" value="PTHR43327:SF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01145">
        <property type="entry name" value="Band_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12221">
        <property type="entry name" value="HflK_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00244">
        <property type="entry name" value="PHB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117892">
        <property type="entry name" value="SSF117892"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01933">
        <property type="entry name" value="hflK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0645">Protease</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="105"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="domain" description="PHB" evidence="7">
        <location>
            <begin position="121"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="35"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="407"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="39"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="63"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="433"/>
            <end position="447"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU364113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ABF08978.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="447" mass="49411" checksum="123405016173870F" modified="2006-05-30" version="1">MPLFPRNPDPHIVPGSRALRFARWPRLRAIFSLNDPRWGRNGQDDEDKDNSRQQNQRPQDGPPDLDELWRDFNRRLNGLLGRKDNGGGGQGFGSGPRPSGKGSNVGIGVIIAAVIGIWLASGFFMVQEGQTAVILQFGKFKYSTGPGINWRMPWPIQSAEVVNLSAVRSVEVGRATSIKDSNLKDSSMLTQDENIIDVRFTVQYDIQDASEFLFFNKTDRGGDEELVTQAAETSVREIVGRNKMDAVLYENREQIAQSLAKSIQSILTAYKTGIRVISVNVQSVQPPEQVQAAFDDVNKASQDRERAISEGQAYANDIIPRAKGTAARLKEESEAYRSRVVAQAEGDAARFRSVQSEYAKAPQVTRDRIYLETMQQIYANSSKILVDAKSGNNLLYLPLDKLMTQVPGSSAAPSSAAPGTNAPAVPDATTPDIRSRESMRNRDRDIR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-01-15" modified="2022-05-25" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LLK0</accession>
    <name>HISZ_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">ATP phosphoribosyltransferase regulatory subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">hisZ</name>
        <name type="ordered locus">Rmet_2097</name>
    </gene>
    <organism>
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Required for the first step of histidine biosynthesis. May allow the feedback regulation of ATP phosphoribosyltransferase activity by histidine.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-histidine biosynthesis; L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 1/9.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Heteromultimer composed of HisG and HisZ subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="miscellaneous">
        <text>This function is generally fulfilled by the C-terminal part of HisG, which is missing in some bacteria such as this one.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-II aminoacyl-tRNA synthetase family. HisZ subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08976.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516810.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q1LLK0"/>
    <dbReference type="SMR" id="Q1LLK0"/>
    <dbReference type="STRING" id="266264.Rmet_2097"/>
    <dbReference type="EnsemblBacteria" id="ABF08976">
        <property type="protein sequence ID" value="ABF08976"/>
        <property type="gene ID" value="Rmet_2097"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2097"/>
    <dbReference type="eggNOG" id="COG3705">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025113_0_1_4"/>
    <dbReference type="OMA" id="ELVMPPM"/>
    <dbReference type="OrthoDB" id="1236894at2"/>
    <dbReference type="UniPathway" id="UPA00031">
        <property type="reaction ID" value="UER00006"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000105">
        <property type="term" value="P:histidine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00773">
        <property type="entry name" value="HisRS-like_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00125">
        <property type="entry name" value="HisZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041715">
        <property type="entry name" value="HisRS-like_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004516">
        <property type="entry name" value="HisRS/HisZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004517">
        <property type="entry name" value="HisZ"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43707">
        <property type="entry name" value="PTHR43707"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13393">
        <property type="entry name" value="tRNA-synt_His"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="SSF55681"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00443">
        <property type="entry name" value="hisZ_biosyn_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0368">Histidine biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_1000016282" description="ATP phosphoribosyltransferase regulatory subunit">
        <location>
            <begin position="1"/>
            <end position="383"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00125"/>
        </source>
    </evidence>
    <sequence length="383" mass="41947" checksum="5D389F57C5DC208D" modified="2006-05-30" version="1">MSNRWLLPENIADVLPSEARKIEELRRRMLDLFRTYGYELVMPPMLEYLESLLTGTGHDLDLRTLKLVDQLSGRTLGLRADITPQVARIDAHLLNRPGVTRLCYAGNVLHARPAGFNATREPIQIGAEIYGHAGLEADVEIQELMLAALQVAGLSDIRLDLCHAGILEALLAALPSIRQIEEAMFAALETKDVPALRELTQGLPDTERDALLALPTLYGGVEVIERARATLPASPAIGRALDELAALAAQVSNASVNIDLSDLRGYHYHSGVMFTAYVSGLPNYVARGGRYDKVGEAFGRARPATGFSLDLREVAALSPVEVRAQAIFAPWDADPALRFAITTLRANGEIVIQSLPGHTHELDEFNCDRQLQRQGANWVVVPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LLP6</accession>
    <name>Q1LLP6_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Glycerophosphodiester phosphodiesterase, cytosolic</fullName>
            <ecNumber evidence="2">3.1.4.46</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">ugpQ</name>
        <name evidence="2" type="ordered locus">Rmet_2051</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EC" id="3.1.4.46" evidence="2"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08930.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516762.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2051"/>
    <dbReference type="EnsemblBacteria" id="ABF08930">
        <property type="protein sequence ID" value="ABF08930"/>
        <property type="gene ID" value="Rmet_2051"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2051"/>
    <dbReference type="eggNOG" id="COG0584">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030006_3_2_4"/>
    <dbReference type="OMA" id="YRMFECD"/>
    <dbReference type="OrthoDB" id="1542724at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008889">
        <property type="term" value="F:glycerophosphodiester phosphodiesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.190">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030395">
        <property type="entry name" value="GP_PDE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017946">
        <property type="entry name" value="PLC-like_Pdiesterase_TIM-brl"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03009">
        <property type="entry name" value="GDPD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51695">
        <property type="entry name" value="SSF51695"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51704">
        <property type="entry name" value="GP_PDE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GP-PDE" evidence="1">
        <location>
            <begin position="7"/>
            <end position="245"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF08930.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="245" mass="26211" checksum="3B1945E9820FB1FF" modified="2006-05-30" version="1">MTNWPYPRFIAHRGAGKLAPENTLAAFRHGASFGYRMFEFDVKLSADGKPILMHDATLDRTTTGKGRVDALTLGELAALDAGGWHSPAYAGEPVPTLAAIARYTQANGFMVNIEIKPVPGTEYRTGAAVALDALSLWRGAGTPPLLSSFSEVALEAAHQVAPDLPRALLLDTLPADWLERLRQLECVALDANHRELDAAVIGAAHSAGFKVLCYTVNDRARAANLLSWGLDGLITDAVDQIAPQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LLX3</accession>
    <name>Q1LLX3_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_1974</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0111 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08853.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008650676.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1974"/>
    <dbReference type="EnsemblBacteria" id="ABF08853">
        <property type="protein sequence ID" value="ABF08853"/>
        <property type="gene ID" value="Rmet_1974"/>
    </dbReference>
    <dbReference type="GeneID" id="60821420"/>
    <dbReference type="KEGG" id="rme:Rmet_1974"/>
    <dbReference type="eggNOG" id="COG1392">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_086031_2_0_4"/>
    <dbReference type="OMA" id="LFNGKYD"/>
    <dbReference type="OrthoDB" id="1837604at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.220">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038078">
        <property type="entry name" value="PhoU-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018445">
        <property type="entry name" value="Put_Phosphate_transp_reg"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01865">
        <property type="entry name" value="PhoU_div"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008591"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF08853.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="208" mass="23502" checksum="2C91E736DD354009" modified="2006-05-30" version="1">MFGRFMPTEGKFFEYFNQHADCAVTAAHELEALVNDLPNAEAHARRVQATEKKADRVTHDTIDLLHKTFITPLDRDEIHKLITTMDDILDLMEDVAETISLYDVTSLTDEARRLAAICVQCCDQVKIAVALLEDMGNASAILKTTQQIDQLESEADRVMRSAMSKLFREETDVKRLIKLKAIYEQLESITDKCEDVANIIEGIVLENA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-10-07" version="59" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LM43</accession>
    <name>Q1LM43_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_1904</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08783.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1904"/>
    <dbReference type="EnsemblBacteria" id="ABF08783">
        <property type="protein sequence ID" value="ABF08783"/>
        <property type="gene ID" value="Rmet_1904"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1904"/>
    <dbReference type="eggNOG" id="COG2067">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_077404_2_0_4"/>
    <dbReference type="OMA" id="TANIWDG"/>
    <dbReference type="OrthoDB" id="1258929at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF08783.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="298" mass="31622" checksum="AE4FA09F3A16A452" modified="2006-05-30" version="1">MELVQRGRRLGRSDIEARISRLRGKRIGVAILFAIATGSGHAESPDAGGWKFAIAPYLWLPNVSGAFSFSGNSSTGGGRLDLGTGPDSYLQNLQFLLMVQAEASKGDWAIIADGAYLDFSHQQTTLNAVGAGGGGAVAIPRELATSTSSSLSGGLFGLAAAYTALRSQWGTVEPLGGLRYLNLSASANWTLSATFTQQGATLATQGSLSQTANIWDGIIGVRGRLRLGNLDRWYVPYYVDVGTGSSRYTVQASGGAAYAASWGDVQLTYRYLTYQLRSGDLVQRLTLKGPMLSVAFRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-10-07" version="41" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LM58</accession>
    <name>Q1LM58_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_1889</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08768.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF08768">
        <property type="protein sequence ID" value="ABF08768"/>
        <property type="gene ID" value="Rmet_1889"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1889"/>
    <dbReference type="HOGENOM" id="CLU_2587189_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF08768.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="80" mass="8696" checksum="AF4A20D6FC4CBE53" modified="2006-05-30" version="1">MSEVEFVSWLRGFVNARGAVPPTAVQWREIMARVSICSVDPLGPKQREAVELSEAIGRLRVAATQQVGVLVPQPLAAARQ</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-10-17" modified="2022-08-03" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LM62</accession>
    <name>CLPP_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">ATP-dependent Clp protease proteolytic subunit</fullName>
            <ecNumber evidence="1">3.4.21.92</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Endopeptidase Clp</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">clpP</name>
        <name type="ordered locus">Rmet_1885</name>
    </gene>
    <organism>
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Cleaves peptides in various proteins in a process that requires ATP hydrolysis. Has a chymotrypsin-like activity. Plays a major role in the degradation of misfolded proteins.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Hydrolysis of proteins to small peptides in the presence of ATP and magnesium. alpha-casein is the usual test substrate. In the absence of ATP, only oligopeptides shorter than five residues are hydrolyzed (such as succinyl-Leu-Tyr-|-NHMec, and Leu-Tyr-Leu-|-Tyr-Trp, in which cleavage of the -Tyr-|-Leu- and -Tyr-|-Trp bonds also occurs).</text>
            <dbReference type="EC" id="3.4.21.92"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Fourteen ClpP subunits assemble into 2 heptameric rings which stack back to back to give a disk-like structure with a central cavity, resembling the structure of eukaryotic proteasomes.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase S14 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.21.92" evidence="1"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08764.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008651661.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q1LM62"/>
    <dbReference type="SMR" id="Q1LM62"/>
    <dbReference type="STRING" id="266264.Rmet_1885"/>
    <dbReference type="MEROPS" id="S14.001"/>
    <dbReference type="EnsemblBacteria" id="ABF08764">
        <property type="protein sequence ID" value="ABF08764"/>
        <property type="gene ID" value="Rmet_1885"/>
    </dbReference>
    <dbReference type="GeneID" id="60821574"/>
    <dbReference type="KEGG" id="rme:Rmet_1885"/>
    <dbReference type="eggNOG" id="COG0740">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_058707_3_2_4"/>
    <dbReference type="OMA" id="RDYWMKA"/>
    <dbReference type="OrthoDB" id="1728970at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004176">
        <property type="term" value="F:ATP-dependent peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd07017">
        <property type="entry name" value="S14_ClpP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00444">
        <property type="entry name" value="ClpP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001907">
        <property type="entry name" value="ClpP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029045">
        <property type="entry name" value="ClpP/crotonase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023562">
        <property type="entry name" value="ClpP/TepA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033135">
        <property type="entry name" value="ClpP_His_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018215">
        <property type="entry name" value="ClpP_Ser_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10381">
        <property type="entry name" value="PTHR10381"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00574">
        <property type="entry name" value="CLP_protease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00127">
        <property type="entry name" value="CLPPROTEASEP"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52096">
        <property type="entry name" value="SSF52096"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00493">
        <property type="entry name" value="clpP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00382">
        <property type="entry name" value="CLP_PROTEASE_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00381">
        <property type="entry name" value="CLP_PROTEASE_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0645">Protease</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0720">Serine protease</keyword>
    <feature type="chain" id="PRO_0000252831" description="ATP-dependent Clp protease proteolytic subunit">
        <location>
            <begin position="1"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="1">
        <location>
            <position position="120"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="145"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00444"/>
        </source>
    </evidence>
    <sequence length="216" mass="23582" checksum="4174832E1DCC00B1" modified="2006-05-30" version="1">MTRNDLLDRLATTQASALETQGLGLVPMVVEQSGRGERAYDIYSRLLKERVVFMVGEVNDQTANLVVAQLLFLESENPDKDVSLYINSPGGSVSAGLAIYDTMQFIKPDVQTLCMGMAASMGAFLLAAGAKGKRSALPNSRIMIHQPLGGARGQASDIEIQAREILYLRERLNSILSEVTGQPVEKIARDTDRDNFMSGDQAVDYGLIDKVITRRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LMA3</accession>
    <name>Q1LMA3_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Macro domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_1844</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08723.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008650222.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1844"/>
    <dbReference type="EnsemblBacteria" id="ABF08723">
        <property type="protein sequence ID" value="ABF08723"/>
        <property type="gene ID" value="Rmet_1844"/>
    </dbReference>
    <dbReference type="GeneID" id="60825663"/>
    <dbReference type="KEGG" id="rme:Rmet_1844"/>
    <dbReference type="eggNOG" id="COG2110">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046550_5_1_4"/>
    <dbReference type="OMA" id="HYGKGLP"/>
    <dbReference type="OrthoDB" id="1812319at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.220.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002589">
        <property type="entry name" value="Macro_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043472">
        <property type="entry name" value="Macro_dom-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01661">
        <property type="entry name" value="Macro"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00506">
        <property type="entry name" value="A1pp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52949">
        <property type="entry name" value="SSF52949"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51154">
        <property type="entry name" value="MACRO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Macro" evidence="1">
        <location>
            <begin position="1"/>
            <end position="173"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF08723.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="173" mass="18265" checksum="D8EB6253876B42E8" modified="2006-05-30" version="1">MTGEHLQVVHGDITRMEVDAIVNAANSGLLGGGGVDGAIHGAGGPAIMDACRAIRDAQGGCPTGEAVITTGGLLPAPYVIHAVGPVWHGGSKEEETQLASAYRNSIRLAGEHNLRTVAFPNISTGIYGFPRERAVDIAIAAVREALPKSPSIEQVTFVCFDDENYRLYRERLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LMJ9</accession>
    <name>Q1LMJ9_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">RND efflux system, outer membrane lipoprotein, NodT</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">agrC</name>
        <name evidence="4" type="ordered locus">Rmet_1748</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the outer membrane factor (OMF) (TC 1.B.17) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08627.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1748"/>
    <dbReference type="EnsemblBacteria" id="ABF08627">
        <property type="protein sequence ID" value="ABF08627"/>
        <property type="gene ID" value="Rmet_1748"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1748"/>
    <dbReference type="eggNOG" id="COG1538">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012817_13_1_4"/>
    <dbReference type="OMA" id="TIRYKEG"/>
    <dbReference type="OrthoDB" id="1619477at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015562">
        <property type="term" value="F:efflux transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003423">
        <property type="entry name" value="OMP_efflux"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010131">
        <property type="entry name" value="RND_efflux_OM_lipoprot_NodT"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02321">
        <property type="entry name" value="OEP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01845">
        <property type="entry name" value="outer_NodT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0564">Palmitate</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1134">Transmembrane beta strand</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5001442052" evidence="2">
        <location>
            <begin position="19"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="468"/>
            <end position="488"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU362097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF08627.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="488" mass="51541" checksum="6331A7DA0DCEA6BC" modified="2006-05-30" version="1">MRLAISAVCIWLAGCSFAPTYHIPEMDLPAKYAEASDPVLSQIPAEWWTSYHDDDLDALIRRIDVTNQTLKRSIALLQDSRAQADAARAAYFPTAGANASATSSHVSANVVGRSLAGKTTPDRLIGFSATWEPDLFGRIGNAVDAATSRVQASADDVAAVRLTLQAEVATDYFAIRSLDREASLLRQTVDAYSSVLSMVTNRYKGGIAAESDVAQAQAQRDSTKAQLADLTLARTQLQHALATLVGEPASTFSIAPHDAYGTQLPVVPAAVPSELLKRRPDIAAVERRVSAANSQIGVAKAAFFPSLMLNLSGGVESSGLAGLLTAPGRFWAVGPALAATIFDGGKRQAGVDSAQAQFDAAAADYRQTVLTAVQDVEDSYAATRLLKEAASSQEAAVQASQRALAQATDRYKKGAAAYLDVNVVEATTLTNERTLESIHRRQLVSSVALFRSLGGGWQAKDGADAQSTAVDPLATSEPAATEGLHLKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LMS9</accession>
    <name>Q1LMS9_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Pili assembly chaperone, required for type 1 fimbriae</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">fimC</name>
        <name evidence="8" type="ordered locus">Rmet_1666</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Periplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the periplasmic pilus chaperone family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08547.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516395.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1666"/>
    <dbReference type="EnsemblBacteria" id="ABF08547">
        <property type="protein sequence ID" value="ABF08547"/>
        <property type="gene ID" value="Rmet_1666"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1666"/>
    <dbReference type="eggNOG" id="COG3121">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_070768_0_1_4"/>
    <dbReference type="OMA" id="YRPENIA"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030288">
        <property type="term" value="C:outer membrane-bounded periplasmic space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061077">
        <property type="term" value="P:chaperone-mediated protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008962">
        <property type="entry name" value="PapD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036316">
        <property type="entry name" value="Pili_assmbl_chap_C_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001829">
        <property type="entry name" value="Pili_assmbl_chaperone_bac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016148">
        <property type="entry name" value="Pili_assmbl_chaperone_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016147">
        <property type="entry name" value="Pili_assmbl_chaperone_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02753">
        <property type="entry name" value="PapD_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00345">
        <property type="entry name" value="PapD_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00969">
        <property type="entry name" value="CHAPERONPILI"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49354">
        <property type="entry name" value="SSF49354"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49584">
        <property type="entry name" value="SSF49584"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0143">Chaperone</keyword>
    <keyword evidence="3" id="KW-0574">Periplasm</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193627" evidence="5">
        <location>
            <begin position="23"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="domain" description="PapD_N" evidence="6">
        <location>
            <begin position="29"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="domain" description="PapD_C" evidence="7">
        <location>
            <begin position="177"/>
            <end position="240"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007399"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022764"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF02753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ABF08547.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="252" mass="27388" checksum="BC27E5F3CD8B22EC" modified="2006-05-30" version="1">MATRHPIPALCAALSVALALQAGVAIGSVTLTGTRVIYDGRSPGESLQFTNQGSSPSVMQVWVDSGNEQSTPKTADAPFIVTPPVFRIGPNAGQTIRLLYMGKDLPKDRESVFYLNTLEIPSLNATYANQNQMLVMLRNRIKILYRPTDIEGSPQKAIEKLSFQIVRQGADVSIVAKNESSYHISLASGNLNCGGDVATFTPGMIPPYSQLEWSVKGRCPIEKSPIRTKVQYVDDYGAVRESEHPVTVEGVH</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LMX2</accession>
    <name>Q1LMX2_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative acetylpolyamine aminohydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_1621</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the histone deacetylase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08504.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516350.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1621"/>
    <dbReference type="EnsemblBacteria" id="ABF08504">
        <property type="protein sequence ID" value="ABF08504"/>
        <property type="gene ID" value="Rmet_1621"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1621"/>
    <dbReference type="eggNOG" id="COG0123">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007727_8_3_4"/>
    <dbReference type="OMA" id="FRAEWIL"/>
    <dbReference type="OrthoDB" id="1443295at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.800.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000286">
        <property type="entry name" value="His_deacetylse"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023801">
        <property type="entry name" value="His_deacetylse_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037138">
        <property type="entry name" value="His_deacetylse_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023696">
        <property type="entry name" value="Ureohydrolase_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00850">
        <property type="entry name" value="Hist_deacetyl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01270">
        <property type="entry name" value="HDASUPER"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52768">
        <property type="entry name" value="SSF52768"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Hist_deacetyl" evidence="2">
        <location>
            <begin position="40"/>
            <end position="350"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF08504.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="357" mass="38501" checksum="37656C2C1DFCAA91" modified="2006-05-30" version="1">MRAFFSDDQLLHQPRQFMRAGRLCEPTDVPARAAVLQRALDARGIELVAPPDYGRAPLETVHSPAYLDFLTSAFERWMAQATPTLEPGIEVLPNLSPYHSGKIDKTRRGPCPSGSVIAEAGYYLGDLSCPLGPHSWQSILRSAHSAVAAARHVCAPHDGNGMAYALCRPSGHHAHADRAGGFCYVNNSAIAAQTLLERFDKVAVLDVDAHHGDGTQQIFYQRSDVMTISLHADPSSYYPFYTGYATERGHGAGYGYNLNFPLPHGTGDAGFLSALDSALDALRDYRPQALVLALGFDTYKHDPISVLQVGLEAYRGVGERIHALGVPTVVVQEGGYEVDAIGIALEAFLAGFVPAMA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="67" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LN24</accession>
    <name>Q1LN24_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_1569</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08452.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516306.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF08452">
        <property type="protein sequence ID" value="ABF08452"/>
        <property type="gene ID" value="Rmet_1569"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1569"/>
    <dbReference type="eggNOG" id="COG3644">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_084995_0_0_4"/>
    <dbReference type="OMA" id="SERAYHF"/>
    <dbReference type="OrthoDB" id="1705862at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018715">
        <property type="entry name" value="DUF2239"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09998">
        <property type="entry name" value="DUF2239"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="68"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="77"/>
            <end position="94"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF08452.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="198" mass="21554" checksum="CE456AF91D1F6FA8" modified="2006-05-30" version="1">MTNPPSYTSFNGHQRIATGPLLANALAVKHALAQGTTGPVLIFDDATGRTLDVDTRGTEQEITSRLQLATPHNKDPDAGLEAPEASEPKGRGRPKLGVVAREVTLLPRHWEWLAGQPGGASVALRKLVEEARRAGSEKDRMRQAHERAYHFMLAIGGDLPGFEEATRALFSNDASRLRELIGGWPADVRDHALHLADY</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LN28</accession>
    <name>Q1LN28_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Flavin-containing monooxygenase FMO:IMP dehydrogenase/GMP reductase:FAD dependent oxidoreductase</fullName>
            <ecNumber evidence="5">1.14.13.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Rmet_1565</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the FAD-binding monooxygenase family.</text>
    </comment>
    <dbReference type="EC" id="1.14.13.-" evidence="5"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08448.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516302.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1565"/>
    <dbReference type="EnsemblBacteria" id="ABF08448">
        <property type="protein sequence ID" value="ABF08448"/>
        <property type="gene ID" value="Rmet_1565"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1565"/>
    <dbReference type="eggNOG" id="COG2072">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032067_2_0_4"/>
    <dbReference type="OMA" id="PWRGPSI"/>
    <dbReference type="OrthoDB" id="324612at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004499">
        <property type="term" value="F:N,N-dimethylaniline monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020946">
        <property type="entry name" value="Flavin_mOase-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00743">
        <property type="entry name" value="FMO-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="SSF51905"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0274">FAD</keyword>
    <keyword evidence="2" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="5" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="4 5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABF08448.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="510" mass="58070" checksum="52B8F828FF8BB4B0" modified="2006-05-30" version="1">MNANILSGRQPARPVATEHFDVIIVGAGISGVGSAYHMRTQCPGKRFVVLESKDSFGGTWHTHRYPGVRSDSDLYTFGYRFKPWMGKPIATAEEILSYMAEVIEENDLAQHIRYRHRIDAASWSSAEGLWTLDTTRLESGERVRFTTNFLWMCQGYYRHDQGYLPEWTGMDNFKGPLIHAQTWPEELDVQGKRVVVIGSGATAATVVPGLARLGAKVTMLQRSPTYFISGRNANDLANELRRLEIDPMWIHAIIRKKVLMDGAIFARRSREEPEEVRKELLAGVRAQLGEDFELEPHFSPRYRPWQQRVAFIPDGDMFAEIREGNAIVVTDEIDRFVEDGVVLKSNEKLDADIVVAATGFQLSVLGDIPFSVDGQSLDFSKTVTYRGMMFTGVPNMVWVFGYFRASWTLRVDMVADFVCRLLKHMDATGAKQVTPQLREDEKDMPLSEWIDADNFNPGYLMRDIQLMPRSGNTREWQHTQDYWGEKDEFPEIDLTDGVFRYDANRPSGNA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="96" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LN85</accession>
    <name>Q1LN85_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">LysR-type transcriptional regulator (RuBisCO operon transcriptional regulator)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">cbbR2</name>
        <name evidence="3" type="ordered locus">Rmet_1508</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the LysR transcriptional regulatory family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08391.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516252.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1508"/>
    <dbReference type="EnsemblBacteria" id="ABF08391">
        <property type="protein sequence ID" value="ABF08391"/>
        <property type="gene ID" value="Rmet_1508"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1508"/>
    <dbReference type="eggNOG" id="COG0583">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039613_6_1_4"/>
    <dbReference type="OMA" id="VCKYYLM"/>
    <dbReference type="OrthoDB" id="750230at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005119">
        <property type="entry name" value="LysR_subst-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000847">
        <property type="entry name" value="Tscrpt_reg_HTH_LysR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00126">
        <property type="entry name" value="HTH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03466">
        <property type="entry name" value="LysR_substrate"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50931">
        <property type="entry name" value="HTH_LYSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH lysR-type" evidence="2">
        <location>
            <begin position="3"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF08391.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="307" mass="34091" checksum="9249927B5BFE3C99" modified="2006-05-30" version="1">MQITFRQLRVVSEVARYSSVIRAAEALHLTAPAVSLQIKEAERQVGLTLFDRAGRRMTLTTTGEYFLVYARRLLATLKDAEDMMARFKRLESGRLTIGMVGAASYFLPQLLAKFHAEHPAVDVRLRLASREKLGVMMQGNDVDLCIMGRPPQDPPTRAEPFASHPHVLVVSPQHRFARAEAVPAVALANEPFIVREPDSGTREALQKYLDAHRIQPTFVMEMPSNEAIKQAVMAGMGVSLLSLHTIALEWSNGLIAVPHVEGLPLERRWNVVNIAAKQLSPAAEAFRYFVLEHGESHLAAMMSHDAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="70" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LN95</accession>
    <name>Q1LN95_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Rubisco activation protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">cbbO</name>
        <name evidence="2" type="ordered locus">Rmet_1498</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08381.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516244.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1498"/>
    <dbReference type="EnsemblBacteria" id="ABF08381">
        <property type="protein sequence ID" value="ABF08381"/>
        <property type="gene ID" value="Rmet_1498"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1498"/>
    <dbReference type="eggNOG" id="COG4548">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020675_0_0_4"/>
    <dbReference type="OMA" id="IHGFCSD"/>
    <dbReference type="OrthoDB" id="39518at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.410">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002035">
        <property type="entry name" value="VWF_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036465">
        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00092">
        <property type="entry name" value="VWA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00327">
        <property type="entry name" value="VWA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53300">
        <property type="entry name" value="SSF53300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50234">
        <property type="entry name" value="VWFA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="VWFA" evidence="1">
        <location>
            <begin position="595"/>
            <end position="783"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF08381.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="785" mass="89216" checksum="8D65E3DF84827774" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="98" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LNI6</accession>
    <name>Q1LNI6_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Putative peptide transport fused subunits of ABC superfamily: ATP-binding components</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">dppD1</name>
        <name evidence="10" type="ordered locus">Rmet_1407</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08290.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516163.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1407"/>
    <dbReference type="EnsemblBacteria" id="ABF08290">
        <property type="protein sequence ID" value="ABF08290"/>
        <property type="gene ID" value="Rmet_1407"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1407"/>
    <dbReference type="eggNOG" id="COG4172">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_86_2_4"/>
    <dbReference type="OMA" id="DPMTRLD"/>
    <dbReference type="OrthoDB" id="1303270at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015833">
        <property type="term" value="P:peptide transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013563">
        <property type="entry name" value="Oligopep_ABC_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08352">
        <property type="entry name" value="oligo_HPY"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01727">
        <property type="entry name" value="oligo_HPY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 8 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-1278">Translocase</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ABC transporter" evidence="9">
        <location>
            <begin position="18"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="9">
        <location>
            <begin position="320"/>
            <end position="570"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="54"/>
            <end position="61"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="363"/>
            <end position="370"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ABF08290.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="630" mass="68243" checksum="EB536772F7EC6D22" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-10-07" version="61" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LNZ3</accession>
    <name>Q1LNZ3_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_1248</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08133.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1248"/>
    <dbReference type="EnsemblBacteria" id="ABF08133">
        <property type="protein sequence ID" value="ABF08133"/>
        <property type="gene ID" value="Rmet_1248"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1248"/>
    <dbReference type="eggNOG" id="COG5489">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_109334_1_0_4"/>
    <dbReference type="OMA" id="GPHFRIF"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007948">
        <property type="entry name" value="DUF736"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05284">
        <property type="entry name" value="DUF736"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF08133.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="117" mass="12556" checksum="FFDC23C68A921AAC" modified="2006-05-30" version="1">MGANLVQPKGANIMANIGTFTADKDGFTGTLRTLTLNVKVKLLPNDKGSSENAPDFRLQAAGHDIGAAWNKTSEAGREYKSVTLDDPSFPAPIYARLIEGEDGTHDLIWSRTKPQAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="104" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LP20</accession>
    <name>Q1LP20_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Benzoyl-CoA oxygenase component A</fullName>
            <ecNumber evidence="5">1.14.13.208</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">boxA</name>
        <name evidence="9" type="ordered locus">Rmet_1220</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>benzoyl-CoA + H(+) + NADPH + O2 = 2,3-epoxy-2,3-dihydrobenzoyl-CoA + H2O + NADP(+)</text>
            <dbReference type="Rhea" id="RHEA:48312"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:57369"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:88118"/>
            <dbReference type="EC" id="1.14.13.208"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="5">Homodimer.</text>
    </comment>
    <dbReference type="EC" id="1.14.13.208" evidence="5"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08106.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515995.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1220"/>
    <dbReference type="EnsemblBacteria" id="ABF08106">
        <property type="protein sequence ID" value="ABF08106"/>
        <property type="gene ID" value="Rmet_1220"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1220"/>
    <dbReference type="eggNOG" id="COG0369">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG1149">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_053066_0_0_4"/>
    <dbReference type="OMA" id="WLFMGVA"/>
    <dbReference type="OrthoDB" id="707164at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017634">
        <property type="entry name" value="Benzoyl_CoA_Oase_BoxA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017927">
        <property type="entry name" value="FAD-bd_FR_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001709">
        <property type="entry name" value="Flavoprot_Pyr_Nucl_cyt_Rdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015701">
        <property type="entry name" value="FNR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039261">
        <property type="entry name" value="FNR_nucleotide-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001433">
        <property type="entry name" value="OxRdtase_FAD/NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017938">
        <property type="entry name" value="Riboflavin_synthase-like_b-brl"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43314">
        <property type="entry name" value="PTHR43314"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00037">
        <property type="entry name" value="Fer4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00175">
        <property type="entry name" value="NAD_binding_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF501177">
        <property type="entry name" value="BoxA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000361">
        <property type="entry name" value="Frd-NADP+_RD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00371">
        <property type="entry name" value="FPNCR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52343">
        <property type="entry name" value="SSF52343"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63380">
        <property type="entry name" value="SSF63380"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03224">
        <property type="entry name" value="benzo_boxA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51384">
        <property type="entry name" value="FAD_FR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0274">FAD</keyword>
    <keyword evidence="5" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="3" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 6" id="KW-0521">NADP</keyword>
    <keyword evidence="5 9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="7">
        <location>
            <begin position="8"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="7">
        <location>
            <begin position="39"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="domain" description="FAD-binding FR-type" evidence="8">
        <location>
            <begin position="145"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="207"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="227"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="282"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="338"/>
            <end position="339"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="374"/>
            <end position="375"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="413"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRNR" id="PIRNR000361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR000361-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ABF08106.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="415" mass="45563" checksum="A5686B7C4E7CB5DC" modified="2006-05-30" version="1">MGAADIIKQHLIDPEICIRCNTCEDTCPIDAITHDDRNYVVKADVCNACNACLSPCPTGAIDNWRTMLKGQAYTIEAQLTWDELPEEVPLPEAEIEAAAAAGQVIEEASRGSKSVAVQEVEASRHTSSRAPWSAAHPYVNLHGVREPVTATVAGNHRLTAEDASSDIHHIVLDFGNHFFPVLEGQAIGIVPPGTDASGKPHYIRMYSVASPRDGERPGYNNLALTVKRVDTDHDGNPVRGVASNFLCDLAKGDPVQVVGPFGSTFLMPNHREASVMMICTGTGSAPMRAMTERMRRNMDHFSGRRLLFFGARNRRELPYFGPLLKLPKDFLDIHFAFSRDPEVPRRYVQDAIREASAQVAALLADPHGHIYICGLKGMEEGVLDAFAEVCATSGQSWQDIEPRLRAEGRLHIETY</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="101" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LP44</accession>
    <name>Q1LP44_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 10">Pyruvate dehydrogenase E1 component</fullName>
            <ecNumber evidence="4 10">1.2.4.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">aceE</name>
        <name evidence="15" type="ordered locus">Rmet_1196</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="16" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2 10">Component of the pyruvate dehydrogenase (PDH) complex, that catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9 10">
            <text>(R)-N(6)-lipoyl-L-lysyl-[dihydrolipoyllysine-residue acetyltransferase] + H(+) + pyruvate = (R)-N(6)-(S(8)-acetyldihydrolipoyl)-L-lysyl-[dihydrolipoyllysine-residue acetyltransferase] + CO2</text>
            <dbReference type="Rhea" id="RHEA:19189"/>
            <dbReference type="Rhea" id="RHEA-COMP:10480"/>
            <dbReference type="Rhea" id="RHEA-COMP:10481"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:83099"/>
            <dbReference type="ChEBI" id="CHEBI:83111"/>
            <dbReference type="EC" id="1.2.4.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="11">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 10">
            <name>thiamine diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58937"/>
        </cofactor>
    </comment>
    <comment type="subunit">
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                <person name="Monsieurs P."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <begin position="224"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="261"/>
            <end position="279"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ABF07901.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="279" mass="29609" checksum="BA14661B762917F8" modified="2006-05-30" version="1">MTTTPSAAASLADRLEALLPQTQCTKCGFDGCRPYAEAMARGEAASNRCPPGGAEGIVRLSAVLGVAPQPLDPERGVEQPRAVARIEESLCIGCTLCIQACPVDAIVGAPKQMHTVLPDWCTGCDLCVTPCPVDCIEMIPVTGEQTGWAAWSQEQADAAHARYAARGVRLVREREENDARLAAKAAAKLEALQAEPVANTAEQEAQARKKAIIQAAIERARQKQAAARPLNTENVSPAVQAQIDAAEARRAKLGMTTHEPAGEQGNKQENKQDNNNPST</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="68" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LPN0</accession>
    <name>Q1LPN0_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_1010</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07896.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515809.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1010"/>
    <dbReference type="EnsemblBacteria" id="ABF07896">
        <property type="protein sequence ID" value="ABF07896"/>
        <property type="gene ID" value="Rmet_1010"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1010"/>
    <dbReference type="eggNOG" id="COG5031">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_080698_1_0_4"/>
    <dbReference type="OMA" id="DIWHVLT"/>
    <dbReference type="OrthoDB" id="738359at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006744">
        <property type="term" value="P:ubiquinone biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007715">
        <property type="entry name" value="Coq4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12922">
        <property type="entry name" value="PTHR12922"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05019">
        <property type="entry name" value="Coq4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF07896.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="241" mass="26867" checksum="8293C9F34F898047" modified="2006-05-30" version="1">MAASTGFNPYKRDLPTAFDAIRKLLADGHDTEQVFRIMRALNGPSMPKNYSRLLSTPEGRRLAYLREELAERLCDPTYIASFAPGTVGAAYRGFLEKTGYSADGLAKVSNLNQEPVVEDAYMWFGRRTRDIHDIWHVLTGYRADESLGEAALVAFSYAQTGGKGWAFIAVAAALKSWRLTGSLAFARAVLEGYRLGKRAKWLLAEDYDVLLNEPIDAARERLGISEAKRYLACNPMEEWSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LPR1</accession>
    <name>Q1LPR1_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Zinc-carboxypeptidase</fullName>
            <ecNumber evidence="2">3.4.17.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_0979</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EC" id="3.4.17.-" evidence="2"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07865.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515783.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0979"/>
    <dbReference type="EnsemblBacteria" id="ABF07865">
        <property type="protein sequence ID" value="ABF07865"/>
        <property type="gene ID" value="Rmet_0979"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0979"/>
    <dbReference type="eggNOG" id="COG2866">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_042358_0_0_4"/>
    <dbReference type="OMA" id="FAYFEPY"/>
    <dbReference type="OrthoDB" id="397741at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004181">
        <property type="term" value="F:metallocarboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040626">
        <property type="entry name" value="Pepdidase_M14_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000834">
        <property type="entry name" value="Peptidase_M14"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18027">
        <property type="entry name" value="Pepdidase_M14_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00246">
        <property type="entry name" value="Peptidase_M14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00631">
        <property type="entry name" value="Zn_pept"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0121">Carboxypeptidase</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase_M14" evidence="1">
        <location>
            <begin position="113"/>
            <end position="377"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00631"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF07865.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="380" mass="42371" checksum="8DBD483F5F1F186E" modified="2006-05-30" version="1">MTSALHISTLFDSGAIEVEALDRADDIRLRIRADSHADFRQWFHFRLQGAAGQDCKLQFLNAGECTYPDGWRDYSVVASYDRAHWFRVPTRYDGTTMTVDFKPEHDSVWFAYFEPYPEERHLSLLASCQRSPLAKLSHLGSTVDGRDLTCVTLGQPGPGKKTVWMIARQHPGESMAEWFCEGVLQRLTGTGMWAGDPVARKLLDRAVFHIVPNMNPDGSARGNLRTNAAGANLNREWMQPSLETSPEVYHVRRAIEATGVDLFFDIHGDEALPYNFVAGSEMLPGFTAAQRDQQSRFIEAFKRATPDFQDVHGYAASKYNADALKLASKYIGHTFGCLALTLEMPFKDNADLPDPVLGWNGAKSGLLGTSMLQAVLEYLG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2009-07-28" modified="2022-05-25" version="100" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LPX2</accession>
    <name>LEU1_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">2-isopropylmalate synthase</fullName>
            <ecNumber evidence="1">2.3.3.13</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Alpha-IPM synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Alpha-isopropylmalate synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">leuA</name>
        <name type="ordered locus">Rmet_0918</name>
    </gene>
    <organism>
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>3-methyl-2-oxobutanoate + acetyl-CoA + H2O = (2S)-2-isopropylmalate + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:21524"/>
            <dbReference type="ChEBI" id="CHEBI:1178"/>
            <dbReference type="ChEBI" id="CHEBI:11851"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="EC" id="2.3.3.13"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-leucine biosynthesis; L-leucine from 3-methyl-2-oxobutanoate: step 1/4.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homotetramer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.3.3.13" evidence="1"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07804.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008643333.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q1LPX2"/>
    <dbReference type="SMR" id="Q1LPX2"/>
    <dbReference type="STRING" id="266264.Rmet_0918"/>
    <dbReference type="EnsemblBacteria" id="ABF07804">
        <property type="protein sequence ID" value="ABF07804"/>
        <property type="gene ID" value="Rmet_0918"/>
    </dbReference>
    <dbReference type="GeneID" id="60825405"/>
    <dbReference type="KEGG" id="rme:Rmet_0918"/>
    <dbReference type="eggNOG" id="COG0119">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022158_0_1_4"/>
    <dbReference type="OMA" id="NTMRMLV"/>
    <dbReference type="OrthoDB" id="840579at2"/>
    <dbReference type="UniPathway" id="UPA00048">
        <property type="reaction ID" value="UER00070"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003852">
        <property type="term" value="F:2-isopropylmalate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009098">
        <property type="term" value="P:leucine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01025">
        <property type="entry name" value="LeuA_type1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013709">
        <property type="entry name" value="2-isopropylmalate_synth_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002034">
        <property type="entry name" value="AIPM/Hcit_synth_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036230">
        <property type="entry name" value="LeuA_allosteric_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005671">
        <property type="entry name" value="LeuA_bact_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000891">
        <property type="entry name" value="PYR_CT"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00682">
        <property type="entry name" value="HMGL-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08502">
        <property type="entry name" value="LeuA_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00917">
        <property type="entry name" value="LeuA_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110921">
        <property type="entry name" value="SSF110921"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00973">
        <property type="entry name" value="leuA_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00815">
        <property type="entry name" value="AIPM_HOMOCIT_SYNTH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00816">
        <property type="entry name" value="AIPM_HOMOCIT_SYNTH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50991">
        <property type="entry name" value="PYR_CT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0100">Branched-chain amino acid biosynthesis</keyword>
    <keyword id="KW-0432">Leucine biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_1000149254" description="2-isopropylmalate synthase">
        <location>
            <begin position="1"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="domain" description="Pyruvate carboxyltransferase" evidence="2">
        <location>
            <begin position="5"/>
            <end position="268"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01151"/>
        </source>
    </evidence>
    <sequence length="513" mass="55793" checksum="9197ECBBEA35B1F2" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-04-07" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LQM1</accession>
    <name>Q1LQM1_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Type IV pilus response regulator PilG</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">pilG</name>
        <name evidence="3" type="ordered locus">Rmet_0669</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07555.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0669"/>
    <dbReference type="EnsemblBacteria" id="ABF07555">
        <property type="protein sequence ID" value="ABF07555"/>
        <property type="gene ID" value="Rmet_0669"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0669"/>
    <dbReference type="eggNOG" id="COG0745">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_69_17_4"/>
    <dbReference type="OMA" id="IIFIDIM"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Response regulatory" evidence="2">
        <location>
            <begin position="38"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="1">
        <location>
            <position position="87"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF07555.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="167" mass="17737" checksum="631F39B8EAC490A6" modified="2006-05-30" version="1">MRVTQTEIDSKSVDGVRIGADAGADEASAVSGRGVSGKILVIDDSSTIRRTAEIFLTNAGYQVMLAEDGFEALAKVGDLRPDVIFCDILMPRLDGYQTCSLIKKSPRFHAIPVIMLSSRDGVFDRSRGKLVGAHNHLAKPFSREALLQAVQSCLTGCVADEGAAVAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="66" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LQN5</accession>
    <name>Q1LQN5_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Rmet_0655</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the glycosyltransferase 87 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07541.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515512.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0655"/>
    <dbReference type="EnsemblBacteria" id="ABF07541">
        <property type="protein sequence ID" value="ABF07541"/>
        <property type="gene ID" value="Rmet_0655"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0655"/>
    <dbReference type="eggNOG" id="ENOG502Z9HB">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037296_1_0_4"/>
    <dbReference type="OMA" id="WYARYAW"/>
    <dbReference type="OrthoDB" id="1533723at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016758">
        <property type="term" value="F:hexosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018584">
        <property type="entry name" value="GT87"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09594">
        <property type="entry name" value="GT87"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="26"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="114"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="155"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="192"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="223"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="286"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="313"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="380"/>
            <end position="398"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00024033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ABF07541.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="412" mass="44262" checksum="45A2AF07EB2B599F" modified="2006-05-30" version="1">MATLQAAARRIRDDASRSHWLDASRLRLYSCVALACYALYFGIWLFRACVLKVPGVFAPGGDFVVFWSTAKLALSSGAAAPYDLGALRHVELSAVPGLAIGDGVLPWLYPPMSLWFVLPFGLLPYGLATFVFLVGGVAWFAWALCRTLSWRDARLAAVAFPGIVVVLATGQNSLWLAGCAGLALTCLRSRPLLAGVLLGVVAMKPQLALMVPVALLCARAWRALGAMVFTTLVLTVASLLVFGSEPFAAFLRNAAMARESVEQGSALMARMPTVFASIKLISGGLLLPYAIHGLVAAAALASVVYAWSRPCSFALRAAVLVVAGLLVPAYLYDYDLVFLGLAIAWLGAHGHRAGWLRGERELLVLLWLMPLWSHVTGPEIGFQPLPLGLMLALALGVWRIRLERMDKASFNA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-10-07" version="44" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LQR1</accession>
    <name>Q1LQR1_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative phospholipase protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_0629</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07515.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0629"/>
    <dbReference type="EnsemblBacteria" id="ABF07515">
        <property type="protein sequence ID" value="ABF07515"/>
        <property type="gene ID" value="Rmet_0629"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0629"/>
    <dbReference type="eggNOG" id="COG1502">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2828116_0_0_4"/>
    <dbReference type="OMA" id="PAKAFMQ"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="37"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF07515.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="66" mass="7809" checksum="60534FE5385A74D7" modified="2006-05-30" version="1">MTRPLRRKLWHDHTKGMGDHDDPAKAFMQWGKIIGENARRKKTGKEAPHASLIEFHRDDPKRTYKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-04-07" version="101" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LR02</accession>
    <name>Q1LR02_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Exodeoxyribonuclease 7 large subunit</fullName>
            <ecNumber evidence="5">3.1.11.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Exodeoxyribonuclease VII large subunit</fullName>
            <shortName evidence="5">Exonuclease VII large subunit</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5 10" type="primary">xseA</name>
        <name evidence="10" type="ordered locus">Rmet_0538</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Bidirectionally degrades single-stranded DNA into large acid-insoluble oligonucleotides, which are then degraded further into small acid-soluble oligonucleotides.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5 6">
            <text>Exonucleolytic cleavage in either 5'- to 3'- or 3'- to 5'-direction to yield nucleoside 5'-phosphates.</text>
            <dbReference type="EC" id="3.1.11.6"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="5">Heterooligomer composed of large and small subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5 6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5 6">Belongs to the XseA family.</text>
    </comment>
    <dbReference type="EC" id="3.1.11.6" evidence="5"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07424.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0538"/>
    <dbReference type="EnsemblBacteria" id="ABF07424">
        <property type="protein sequence ID" value="ABF07424"/>
        <property type="gene ID" value="Rmet_0538"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0538"/>
    <dbReference type="eggNOG" id="COG1570">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023625_3_1_4"/>
    <dbReference type="OMA" id="WPAVRFE"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009318">
        <property type="term" value="C:exodeoxyribonuclease VII complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008855">
        <property type="term" value="F:exodeoxyribonuclease VII activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006308">
        <property type="term" value="P:DNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd04489">
        <property type="entry name" value="ExoVII_LU_OBF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00378">
        <property type="entry name" value="Exonuc_7_L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003753">
        <property type="entry name" value="Exonuc_VII_L"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020579">
        <property type="entry name" value="Exonuc_VII_lsu_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025824">
        <property type="entry name" value="OB-fold_nuc-bd_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30008">
        <property type="entry name" value="PTHR30008"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02601">
        <property type="entry name" value="Exonuc_VII_L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13742">
        <property type="entry name" value="tRNA_anti_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00237">
        <property type="entry name" value="xseA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 5 6" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="3 5 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 5 6" id="KW-0540">Nuclease</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="tRNA_anti_2" evidence="9">
        <location>
            <begin position="44"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="domain" description="Exonuc_VII_L" evidence="8">
        <location>
            <begin position="159"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="19"/>
            <end position="38"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00378"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU004355"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF13742"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ABF07424.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="481" mass="53157" checksum="890C11DD90BDA02E" modified="2006-05-30" version="1">MTLPVCCGHVHPRPRARLRTMPDNENFPRAMPSQPPTRTRDVIQVSELNHAIAGVLERSFPLAWVRGEISNFTRAASGHWYFSLKDARAQIRCVMFRGRNQHVDFSPREGEAVEVRALVSMYEARGELQLGVEAMRRAGLGNLYEAFLRLKEKLAQAGLFDTERKRPIPDHPRVIGVVTSLQAAAMRDVLTTLQRRAPHVPVIVYPVPVQGAGAAEKIAAMLDTASARRECDVLILCRGGGSIEDLWSFNEEVVAHAIARCAVPVVSGVGHETDFTIADFVADVRAPTPTGAAELVSPDRAHLLQATRRARDALAQAMDRQLERRAQHLEWLGRRLRSPQAQLQERRAQVDNLARHMRSALRDTVAVQRHRVDVLAMRWRACKPDIGAAQAELGRLAARMAAAAARRHERETARLARASGALELLAPQRTLERGYAVLLDNRGRALRSPVELRAGSIVEAHLADGVADIEIAGVQAKLASF</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2007-03-20" modified="2022-05-25" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LRA4</accession>
    <name>LPTD_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">LPS-assembly protein LptD</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">lptD</name>
        <name type="synonym">imp</name>
        <name type="synonym">ostA</name>
        <name type="ordered locus">Rmet_0436</name>
    </gene>
    <organism>
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Together with LptE, is involved in the assembly of lipopolysaccharide (LPS) at the surface of the outer membrane.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Component of the lipopolysaccharide transport and assembly complex. Interacts with LptE and LptA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell outer membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the LptD family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07322.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515310.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q1LRA4"/>
    <dbReference type="SMR" id="Q1LRA4"/>
    <dbReference type="STRING" id="266264.Rmet_0436"/>
    <dbReference type="EnsemblBacteria" id="ABF07322">
        <property type="protein sequence ID" value="ABF07322"/>
        <property type="gene ID" value="Rmet_0436"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0436"/>
    <dbReference type="eggNOG" id="COG1452">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009039_0_0_4"/>
    <dbReference type="OMA" id="DYSHLDW"/>
    <dbReference type="OrthoDB" id="100018at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009279">
        <property type="term" value="C:cell outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043165">
        <property type="term" value="P:Gram-negative-bacterium-type cell outer membrane assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015920">
        <property type="term" value="P:lipopolysaccharide transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010033">
        <property type="term" value="P:response to organic substance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01411">
        <property type="entry name" value="LPS_assembly_LptD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020889">
        <property type="entry name" value="LipoPS_assembly_LptD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007543">
        <property type="entry name" value="LptD_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04453">
        <property type="entry name" value="LptD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0998">Cell outer membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5000118484" description="LPS-assembly protein LptD">
        <location>
            <begin position="49"/>
            <end position="824"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="67"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="91"/>
            <end position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01411"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="824" mass="91744" checksum="455B2DC58AA5187F" modified="2006-05-30" version="1" precursor="true"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-10-31" modified="2022-08-03" version="106" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LRT5</accession>
    <name>GPDA_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Glycerol-3-phosphate dehydrogenase [NAD(P)+]</fullName>
            <ecNumber evidence="1">1.1.1.94</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">NAD(P)H-dependent glycerol-3-phosphate dehydrogenase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">gpsA</name>
        <name type="ordered locus">Rmet_0255</name>
    </gene>
    <organism>
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>NAD(+) + sn-glycerol 3-phosphate = dihydroxyacetone phosphate + H(+) + NADH</text>
            <dbReference type="Rhea" id="RHEA:11092"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57597"/>
            <dbReference type="ChEBI" id="CHEBI:57642"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.1.1.94"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>NADP(+) + sn-glycerol 3-phosphate = dihydroxyacetone phosphate + H(+) + NADPH</text>
            <dbReference type="Rhea" id="RHEA:11096"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57597"/>
            <dbReference type="ChEBI" id="CHEBI:57642"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.1.1.94"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Membrane lipid metabolism; glycerophospholipid metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the NAD-dependent glycerol-3-phosphate dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.94" evidence="1"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07141.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008642656.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q1LRT5"/>
    <dbReference type="SMR" id="Q1LRT5"/>
    <dbReference type="STRING" id="266264.Rmet_0255"/>
    <dbReference type="EnsemblBacteria" id="ABF07141">
        <property type="protein sequence ID" value="ABF07141"/>
        <property type="gene ID" value="Rmet_0255"/>
    </dbReference>
    <dbReference type="GeneID" id="60822980"/>
    <dbReference type="KEGG" id="rme:Rmet_0255"/>
    <dbReference type="eggNOG" id="COG0240">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033449_0_2_4"/>
    <dbReference type="OMA" id="NRMFGNM"/>
    <dbReference type="OrthoDB" id="1419877at2"/>
    <dbReference type="UniPathway" id="UPA00940"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009331">
        <property type="term" value="C:glycerol-3-phosphate dehydrogenase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004367">
        <property type="term" value="F:glycerol-3-phosphate dehydrogenase [NAD+] activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046167">
        <property type="term" value="P:glycerol-3-phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046168">
        <property type="term" value="P:glycerol-3-phosphate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006650">
        <property type="term" value="P:glycerophospholipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008654">
        <property type="term" value="P:phospholipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1040.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00394">
        <property type="entry name" value="NAD_Glyc3P_dehydrog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008927">
        <property type="entry name" value="6-PGluconate_DH-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013328">
        <property type="entry name" value="6PGD_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006168">
        <property type="entry name" value="G3P_DH_NAD-dep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006109">
        <property type="entry name" value="G3P_DH_NAD-dep_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011128">
        <property type="entry name" value="G3P_DH_NAD-dep_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07479">
        <property type="entry name" value="NAD_Gly3P_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01210">
        <property type="entry name" value="NAD_Gly3P_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000114">
        <property type="entry name" value="Glycerol-3-P_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00077">
        <property type="entry name" value="GPDHDRGNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48179">
        <property type="entry name" value="SSF48179"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00957">
        <property type="entry name" value="NAD_G3PDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0444">Lipid biosynthesis</keyword>
    <keyword id="KW-0443">Lipid metabolism</keyword>
    <keyword id="KW-0520">NAD</keyword>
    <keyword id="KW-0560">Oxidoreductase</keyword>
    <keyword id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword id="KW-1208">Phospholipid metabolism</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000255351" description="Glycerol-3-phosphate dehydrogenase [NAD(P)+]">
        <location>
            <begin position="1"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="1">
        <location>
            <position position="202"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="7"/>
            <end position="12"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="112"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="112"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="151"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="266"/>
            <end position="267"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="266"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="292"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00394"/>
        </source>
    </evidence>
    <sequence length="342" mass="35365" checksum="7235098160D2614A" modified="2006-05-30" version="1">MKLTILGAGAWGTALASHAATAAADHDVVLWGRDPAQLAGIAATGVNATYLPGVSLSPRLVCEPDFDRAVDHALTDPEGLLIIGTPVSGLREMTRRLAARRKGPLAMLWLCKGFEAETHAMPHQMVHDELNALGVAPGEIEYGVLTGPSFAKEVAQGLPCALTVAGTAHVLIDRAQAAFHHHAMRIYGSDDLIGVEVGGAVKNVLAIATGASDGLGLGLNARAALITRGLAEMTRLGLALGGRVETFMGLAGMGDLILTATGDLSRNRKVGQQLAGGKTLDQILADLGHVAEGVRCAQAVAALAARVGVEMPITRAVCAVLFEGLPVADAVSQLLQRDARDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LS21</accession>
    <name>Q1LS21_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Bifunctional hydroxy-methylpyrimidine kinase and hydroxy-phosphomethylpyrimidine kinase</fullName>
            <ecNumber evidence="5">2.7.1.49</ecNumber>
            <ecNumber evidence="5">2.7.4.7</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">thiD</name>
        <name evidence="5" type="ordered locus">Rmet_0169</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>4-amino-2-methyl-5-(phosphooxymethyl)pyrimidine + ATP = 4-amino-2-methyl-5-(diphosphooxymethyl)pyrimidine + ADP</text>
            <dbReference type="Rhea" id="RHEA:19893"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57841"/>
            <dbReference type="ChEBI" id="CHEBI:58354"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.7"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>4-amino-5-hydroxymethyl-2-methylpyrimidine + ATP = 4-amino-2-methyl-5-(phosphooxymethyl)pyrimidine + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:23096"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16892"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58354"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.49"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Cofactor biosynthesis; thiamine diphosphate biosynthesis; 4-amino-2-methyl-5-diphosphomethylpyrimidine from 5-amino-1-(5-phospho-D-ribosyl)imidazole: step 3/3.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.49" evidence="5"/>
    <dbReference type="EC" id="2.7.4.7" evidence="5"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07055.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515079.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0169"/>
    <dbReference type="EnsemblBacteria" id="ABF07055">
        <property type="protein sequence ID" value="ABF07055"/>
        <property type="gene ID" value="Rmet_0169"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0169"/>
    <dbReference type="eggNOG" id="COG0351">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020520_0_1_4"/>
    <dbReference type="OMA" id="DNRHTHG"/>
    <dbReference type="OrthoDB" id="461201at2"/>
    <dbReference type="UniPathway" id="UPA00060">
        <property type="reaction ID" value="UER00138"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008902">
        <property type="term" value="F:hydroxymethylpyrimidine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008972">
        <property type="term" value="F:phosphomethylpyrimidine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009228">
        <property type="term" value="P:thiamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009229">
        <property type="term" value="P:thiamine diphosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd01169">
        <property type="entry name" value="HMPP_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1190.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045029">
        <property type="entry name" value="HMP/HMP-P_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004399">
        <property type="entry name" value="HMP/HMP-P_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013749">
        <property type="entry name" value="PM/HMP-P_kinase-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029056">
        <property type="entry name" value="Ribokinase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20858">
        <property type="entry name" value="PTHR20858"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08543">
        <property type="entry name" value="Phos_pyr_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53613">
        <property type="entry name" value="SSF53613"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00097">
        <property type="entry name" value="HMP-P_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Phos_pyr_kin" evidence="4">
        <location>
            <begin position="15"/>
            <end position="267"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00000565"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF08543"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABF07055.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="276" mass="27872" checksum="2189D8EBA1C32717" modified="2006-05-30" version="1">MLPTPPRTLTIAGSDSGGGAGIQADLKTFAALGCFGMSAITAITAQNTLGVTGVHAIPAEMVAAQIDAVASDIGVDAAKTGMLGTAAIVEAVAGAVDRHGIRKLVVDPVMISTSGATLSDDATSQAMVKLLFPRAMLVTPNLPEASYLLGRRITRRAEMEAAAADLLALGCKAVLLKGGHLEDDGPGSLGLDDLLMLADGTVRVYTHPRVDTPNLHGTGCTLAAAIAAQLARGDALPDAVAAALDFVAQAIRSGARLRLGDGNGPLNHSFAPRALG</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LS24</accession>
    <name>Q1LS24_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Thiamine monophosphate synthase</fullName>
            <ecNumber evidence="2">2.5.1.3</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">thiE</name>
        <name evidence="2" type="ordered locus">Rmet_0166</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.5.1.3" evidence="2"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07052.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515077.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0166"/>
    <dbReference type="EnsemblBacteria" id="ABF07052">
        <property type="protein sequence ID" value="ABF07052"/>
        <property type="gene ID" value="Rmet_0166"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0166"/>
    <dbReference type="eggNOG" id="COG0352">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018272_0_1_4"/>
    <dbReference type="OMA" id="NDHWQIA"/>
    <dbReference type="OrthoDB" id="461201at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004789">
        <property type="term" value="F:thiamine-phosphate diphosphorylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd00564">
        <property type="entry name" value="TMP_TenI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036206">
        <property type="entry name" value="ThiamineP_synth_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022998">
        <property type="entry name" value="ThiamineP_synth_TenI"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02581">
        <property type="entry name" value="TMP-TENI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51391">
        <property type="entry name" value="SSF51391"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="TMP-TENI" evidence="1">
        <location>
            <begin position="180"/>
            <end position="358"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02581"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF07052.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="376" mass="39588" checksum="EEABAB48D6220266" modified="2006-05-30" version="1">MTRQGVAAAIDAALFDDLMARYAAMFGRDETPWRIWSAANAPAALDRHDVVLSDGEATGDLIDRVVAADAVLIESEREGGRWIDTVRSPMGTWVLDVRADDDTPHSAAFVAVLRAALSLHFPAHDALCIARAWLPGATAWPNDFARFPQVRHAALVPPGQTAQPFAPCPPDLGLYAVMPSAAWIETLVPLAVPTVQLRFKSGDADAVKHEVARAAKAAKGSQSRLFINDHWRVAIDHHAACGGDSGIYGIHLGQEDLDDADLDAIRASGLRLGVSTHGYAEMLRVAAISPSYLALGAIFPTTTKVMPTQPQGMGRFQSYVALMQPVIPSLVGIGGVNAGNMGEVLAVGVGSAAVVRAITEASDVPAAVAGLNALFA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="70" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LS63</accession>
    <name>Q1LS63_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Cobalamin synthesis protein, P47K (Putative GTPase)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">yjiA</name>
        <name evidence="3" type="ordered locus">Rmet_0127</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SIMIBI class G3E GTPase family. CobW subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07013.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515045.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0127"/>
    <dbReference type="EnsemblBacteria" id="ABF07013">
        <property type="protein sequence ID" value="ABF07013"/>
        <property type="gene ID" value="Rmet_0127"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0127"/>
    <dbReference type="eggNOG" id="COG0523">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017452_0_0_4"/>
    <dbReference type="OMA" id="MWGVLRY"/>
    <dbReference type="OrthoDB" id="1267701at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1220.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011629">
        <property type="entry name" value="Cbl_biosynth_CobW-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036627">
        <property type="entry name" value="CobW-likC_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003495">
        <property type="entry name" value="CobW/HypB/UreG_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02492">
        <property type="entry name" value="cobW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07683">
        <property type="entry name" value="CobW_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00833">
        <property type="entry name" value="CobW_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CobW C-terminal" evidence="2">
        <location>
            <begin position="269"/>
            <end position="362"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF07013.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="363" mass="40621" checksum="2B9BBF62AFF755A2" modified="2006-05-30" version="1">MSKLIPVTILTGFLGSGKTTLLKRILNEQHGMKIAVIENEFGEENIDNEILVQDGREQIVQMSNGCICCTIRGDLVQALSDLVTQRDEGKIAFDRVVIETTGVANPGPVAQTFFMDEEIASRYLLDAVITLVDAKHANLQLDKQEEAQRQVGFADAIFITKADLVSEAEVADLRHRLLHMNPRAPINAAHFGEAPIDLIFDLRGFNLNEKLEIDPDFLRADEHDHEHDHEHGEHCGTDCGHDHGHEHGHDHGHGHAHDHHHHHHHTDRIASFVFRSDKPFHYGKLEEFLSGILSVYGEKLLRYKGVLYMEGVDRKVVFQGVHQLMGSDIGAKWEDEQPGTKMVFIGVDLPRDAILKGLAACLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LSB9</accession>
    <name>Q1LSB9_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="9">ABC-type transporter, ATPase component</fullName>
            <ecNumber evidence="9">3.6.3.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">Rmet_0071</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EC" id="3.6.3.-" evidence="9"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF06957.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008648922.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0071"/>
    <dbReference type="EnsemblBacteria" id="ABF06957">
        <property type="protein sequence ID" value="ABF06957"/>
        <property type="gene ID" value="Rmet_0071"/>
    </dbReference>
    <dbReference type="GeneID" id="60822793"/>
    <dbReference type="KEGG" id="rme:Rmet_0071"/>
    <dbReference type="eggNOG" id="COG1127">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_22_4"/>
    <dbReference type="OMA" id="RMKDFDA"/>
    <dbReference type="OrthoDB" id="1058434at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="1" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-1278">Translocase</keyword>
    <feature type="domain" description="ABC transporter" evidence="8">
        <location>
            <begin position="15"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="47"/>
            <end position="54"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ABF06957.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="279" mass="30337" checksum="5DADAF7F1D49D7BE" modified="2006-05-30" version="1">MSPTIERGTPRETIIEVRNLEKRFGDNVVHKDLNLEVYRGEVLSIVGGSGSGKTVLLRQIVGLDRPTSGTIRVFGENPAELSADQLQALRSRWGLQFQRGALFSALSVIDNVALPLREMRALPDDLICRASLLKLQLVGLSAKDADKMPSDLSGGMIKRVGLARALALEPELVFLDEPTAGLDPMASDDYVALIRELRRELGLTVVMITHDLDTLVALSDRVAVLADHRVLAAAPIPEVIKVDHPFIREYFLGERAQRALQALPAPKGPGAPDAHPGEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="58" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LSC5</accession>
    <name>Q1LSC5_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_0065</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF06951.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011514990.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0065"/>
    <dbReference type="EnsemblBacteria" id="ABF06951">
        <property type="protein sequence ID" value="ABF06951"/>
        <property type="gene ID" value="Rmet_0065"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0065"/>
    <dbReference type="eggNOG" id="COG3819">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_099769_0_0_4"/>
    <dbReference type="OMA" id="CAFLIHG"/>
    <dbReference type="OrthoDB" id="1682250at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010374">
        <property type="entry name" value="DUF969"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06149">
        <property type="entry name" value="DUF969"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="29"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="59"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="171"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="197"/>
            <end position="214"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF06951.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="244" mass="26004" checksum="2D7DA9237E9DF7A2" modified="2006-05-30" version="1">METALNLWPLLGVGVIILGFVLRFNPMMVVAAAAIVTGLAASMPVMQIFTAIGTAFVKARNLPLIILLPLAVIGLLERHGLREHAQAWISRIASATVGRLLIVYLGVRELTAAVGLTSLGGHPQMVRPLLAPMAEGAAENRFGHLPEPVRQRILAFCAATDNVGLFFGEDIFVAFGAIALMHTFLLSSNIDVEPLHIAVWGIPTAICAFLIHAVRLKRLDGWLERELGGKQAAGTPATAVAKGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-04-26" modified="2021-09-29" version="113" xmlns="http://uniprot.org/uniprot">
    <accession>Q58AJ4</accession>
    <name>Q58AJ4_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="14">PbrT, Pb(II)-uptake protein involved in Pb(II) resistance</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">pbrT</name>
        <name evidence="14" type="ordered locus">Rmet_5945</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="14 15">
        <name>pMOL30</name>
    </geneLocation>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="15">Plasmid pMOL30</plasmid>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the oxidase-dependent Fe transporter (OFeT) (TC 9.A.10.1) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000354">
        <property type="protein sequence ID" value="ABF12804.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008645747.1">
        <property type="nucleotide sequence ID" value="NC_007971.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_145624.1">
        <property type="nucleotide sequence ID" value="NC_006466.1"/>
    </dbReference>
    <dbReference type="SMR" id="Q58AJ4"/>
    <dbReference type="TCDB" id="2.A.108.2.1">
        <property type="family name" value="the iron/lead transporter (ilt) family"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF12804">
        <property type="protein sequence ID" value="ABF12804"/>
        <property type="gene ID" value="Rmet_5945"/>
    </dbReference>
    <dbReference type="GeneID" id="60823113"/>
    <dbReference type="KEGG" id="rme:Rmet_5945"/>
    <dbReference type="HOGENOM" id="CLU_027143_0_0_4"/>
    <dbReference type="OMA" id="MHDRRHA"/>
    <dbReference type="OrthoDB" id="433945at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid pMOL30"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033573">
        <property type="term" value="C:high-affinity iron permease complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005381">
        <property type="term" value="F:iron ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.760.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009056">
        <property type="entry name" value="Cyt_c-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036909">
        <property type="entry name" value="Cyt_c-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004923">
        <property type="entry name" value="FTR1/Fip1/EfeU"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31632">
        <property type="entry name" value="PTHR31632"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13442">
        <property type="entry name" value="Cytochrome_CBB3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03239">
        <property type="entry name" value="FTR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46626">
        <property type="entry name" value="SSF46626"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51007">
        <property type="entry name" value="CYTC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3 9" id="KW-0349">Heme</keyword>
    <keyword evidence="7 9" id="KW-0408">Iron</keyword>
    <keyword evidence="8 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-0614">Plasmid</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
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        <location>
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            <end position="445"/>
        </location>
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        <location>
            <begin position="457"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="495"/>
            <end position="512"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="532"/>
            <end position="553"/>
        </location>
    </feature>
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        <location>
            <begin position="565"/>
            <end position="587"/>
        </location>
    </feature>
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        <location>
            <begin position="607"/>
            <end position="631"/>
        </location>
    </feature>
    <feature type="domain" description="Cytochrome c" evidence="13">
        <location>
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            <end position="276"/>
        </location>
    </feature>
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        <location>
            <begin position="355"/>
            <end position="382"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008333"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS51007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ABF12804.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="642" mass="68264" checksum="EA3D7B9A5DB995B4" modified="2005-04-26" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2005-03-01" modified="2022-01-19" version="98" xmlns="http://uniprot.org/uniprot">
    <accession>Q5NUX6</accession>
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    </organism>
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        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
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        </source>
    </reference>
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    </dbReference>
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    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF13070">
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        <property type="gene ID" value="Rmet_6211"/>
    </dbReference>
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    <dbReference type="KEGG" id="rme:Rmet_6211"/>
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    <dbReference type="OMA" id="EPHSHWH"/>
    <dbReference type="OrthoDB" id="1515911at2"/>
    <dbReference type="Proteomes" id="UP000002429">
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    </dbReference>
    <dbReference type="GO" id="GO:0016021">
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="entry name" value="EamA_dom"/>
    </dbReference>
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        <property type="entry name" value="EamA"/>
        <property type="match status" value="2"/>
    </dbReference>
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    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
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        <location>
            <begin position="33"/>
            <end position="53"/>
        </location>
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        </source>
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    </evidence>
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        <source>
            <dbReference type="EMBL" id="ABF13070.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2005-01-04" modified="2021-09-29" version="88" xmlns="http://uniprot.org/uniprot">
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        <submittedName>
            <fullName evidence="5">Mating pair formation</fullName>
        </submittedName>
    </protein>
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        <name evidence="5" type="primary">trbG</name>
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        <name>pMOL28</name>
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    <reference evidence="6" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
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                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="6">Plasmid pMOL28</plasmid>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TrbG/VirB9 family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000355">
        <property type="protein sequence ID" value="ABF13159.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_007972.2"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_006525.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF13159">
        <property type="protein sequence ID" value="ABF13159"/>
        <property type="gene ID" value="Rmet_6300"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6300"/>
    <dbReference type="HOGENOM" id="CLU_058585_0_1_4"/>
    <dbReference type="OMA" id="DPITMPG"/>
    <dbReference type="OrthoDB" id="1251301at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid pMOL28"/>
    </dbReference>
    <dbReference type="CDD" id="cd06911">
        <property type="entry name" value="VirB9_CagX_TrbG"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010258">
        <property type="entry name" value="Conjugal_tfr_TrbG/VirB9/CagX"/>
    </dbReference>
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        <property type="entry name" value="VirB9/CagX/TrbG_C"/>
    </dbReference>
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        <property type="entry name" value="VirB9/CagX/TrbG_C_sf"/>
    </dbReference>
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        <property type="entry name" value="CagX"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
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        <location>
            <begin position="30"/>
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        </location>
    </feature>
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        <location>
            <begin position="181"/>
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        </location>
    </feature>
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        <location>
            <begin position="181"/>
            <end position="198"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00006135"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABF13159.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="303" mass="32827" checksum="75B25D56174BFCED" modified="2005-01-04" version="1">MEARTPSRFIPLAIALCMAMLLSAPDSHAADKNARRTGAGMAGLGIGDLSDAMNPANPFNGGVDPIALPGDSRLVVFTYSRDQIFRVLSAPLKITTIEFPDDEQIVGDPAWGENVRWDYDTDGANHLYVKPQAAGLVNTLSVNTNKRSYEFTLVSSPLGGIFYQKVRFKVPTSISAKVKARNDARGEAPDEAKGAEAEGSRNPDAVGVSPDQLNFEYAISGSASFKPETAFDDGKALWLRLPNAALQNWPVAFIKDGSDFVVVNAIRRGGYLVVQRLADTVVLRSGDDEVKIERGRRRILGLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-03-23" modified="2020-10-07" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>D3DXR4</accession>
    <name>D3DXR4_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name evidence="1" type="ordered locus">Rmet_6475</name>
    </gene>
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        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
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        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ADC45084.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_6475"/>
    <dbReference type="EnsemblBacteria" id="ADC45084">
        <property type="protein sequence ID" value="ADC45084"/>
        <property type="gene ID" value="Rmet_6475"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6475"/>
    <dbReference type="HOGENOM" id="CLU_3426660_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADC45084.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="21" mass="2320" checksum="8799489D24F4F208" modified="2010-03-23" version="1">MAFVCMVIARGMCFADPTRPC</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-03-23" modified="2020-10-07" version="29" xmlns="http://uniprot.org/uniprot">
    <accession>D3DXR7</accession>
    <name>D3DXR7_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_6479</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ADC45087.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_6479"/>
    <dbReference type="EnsemblBacteria" id="ADC45087">
        <property type="protein sequence ID" value="ADC45087"/>
        <property type="gene ID" value="Rmet_6479"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6479"/>
    <dbReference type="HOGENOM" id="CLU_2772944_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="28"/>
            <end position="47"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADC45087.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="69" mass="7216" checksum="DCF35EAEAAE1DEC4" modified="2010-03-23" version="1">MVRVPPACSKCCKASRAHTVFPLSHAMGEGRGSELSGSGGDLSQAAPQRMLPQPLCRLAGEGRFSPTAY</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-03-23" modified="2020-10-07" version="30" xmlns="http://uniprot.org/uniprot">
    <accession>D3DY05</accession>
    <name>D3DY05_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_6573</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ADC45175.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_6573"/>
    <dbReference type="EnsemblBacteria" id="ADC45175">
        <property type="protein sequence ID" value="ADC45175"/>
        <property type="gene ID" value="Rmet_6573"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6573"/>
    <dbReference type="HOGENOM" id="CLU_2181744_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="66"/>
            <end position="109"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADC45175.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="109" mass="11975" checksum="05438F8FE081BD12" modified="2010-03-23" version="1">MRNAGEWEAKRDASCMVFLLNKKAVEENRTPDSKIRSPAFRLFGAVGARNPVGPVATVFPEFIARDERERADGGRQGNKRRVGRPAGAAEDTALRALTAHGRGLQSRTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-03-23" modified="2020-10-07" version="25" xmlns="http://uniprot.org/uniprot">
    <accession>D3DYB7</accession>
    <name>D3DYB7_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_6705</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ADC45287.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_6705"/>
    <dbReference type="EnsemblBacteria" id="ADC45287">
        <property type="protein sequence ID" value="ADC45287"/>
        <property type="gene ID" value="Rmet_6705"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6705"/>
    <dbReference type="HOGENOM" id="CLU_1904992_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADC45287.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="133" mass="13231" checksum="FC072A1323DEDF05" modified="2010-03-23" version="1">MLSLIVDGMLAVPAVMIGGDVMVVGAPPSAKADLSSALVVCNSVTVAGGLVVVGGVTGVTGVDGVVPGAAGLAVPELPPEACVVPSPPPPPQPDTSPTARAIDKAASFDFIPFSFLFLNYLHEVSTAALRQRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-03-23" modified="2020-02-26" version="17" xmlns="http://uniprot.org/uniprot">
    <accession>D3DYD6</accession>
    <name>D3DYD6_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_6727</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ADC45306.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADC45306">
        <property type="protein sequence ID" value="ADC45306"/>
        <property type="gene ID" value="Rmet_6727"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6727"/>
    <dbReference type="HOGENOM" id="CLU_3419136_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADC45306.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="25" mass="3370" checksum="2BDC04CC4C60FE4C" modified="2010-03-23" version="1">MQKRREWKLSIRELRFDLFWLKRYA</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-03-23" modified="2020-10-07" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>D3DYF7</accession>
    <name>D3DYF7_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_6748</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ADC45327.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_6748"/>
    <dbReference type="EnsemblBacteria" id="ADC45327">
        <property type="protein sequence ID" value="ADC45327"/>
        <property type="gene ID" value="Rmet_6748"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6748"/>
    <dbReference type="HOGENOM" id="CLU_3383438_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADC45327.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="33" mass="3754" checksum="A0F9AFE77BBD1791" modified="2010-03-23" version="1">MKLSAYFKPVFSAGFKALHERLIWALSPAKFTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-03-23" modified="2021-06-02" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>D3DYG9</accession>
    <name>D3DYG9_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_6760</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ADC45339.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADC45339">
        <property type="protein sequence ID" value="ADC45339"/>
        <property type="gene ID" value="Rmet_6760"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6760"/>
    <dbReference type="HOGENOM" id="CLU_1925844_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="93"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="93"/>
            <end position="120"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ADC45339.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="131" mass="14365" checksum="6184ADD17CD1EE37" modified="2010-03-23" version="1">MDSFLIDHFAGKSTGSVIRAKHADLTVRLASAHGSHTWRSQAQATVHCGPCGHERQSIRGFNAQVRKLPEIGTGRLMFNQGVHAARTCGMDWHGDRLEGNLRTPSLRPRERGKHAGESVTGRERPLPPRAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-03-23" modified="2021-06-02" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>D3DYJ6</accession>
    <name>D3DYJ6_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_6396</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="1 2">
        <name>pMOL28</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid pMOL28</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000355">
        <property type="protein sequence ID" value="ADC45366.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011514935.1">
        <property type="nucleotide sequence ID" value="NC_007972.2"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ADC45366">
        <property type="protein sequence ID" value="ADC45366"/>
        <property type="gene ID" value="Rmet_6396"/>
    </dbReference>
    <dbReference type="GeneID" id="60824657"/>
    <dbReference type="KEGG" id="rme:Rmet_6396"/>
    <dbReference type="HOGENOM" id="CLU_2737171_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid pMOL28"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADC45366.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="71" mass="8138" checksum="062425B4A526CBA7" modified="2010-03-23" version="1">MTKTYEIWQAISDIDGSGQNALIEKGQFEAQAHLFEGTPVLLKTFDAETYDEAAQIYNDYFGWGKYHPMKD</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1990-01-01" modified="2022-05-25" version="134" xmlns="http://uniprot.org/uniprot">
    <accession>P13510</accession>
    <accession>Q58AM4</accession>
    <name>CZCB_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName>Cobalt-zinc-cadmium resistance protein CzcB</fullName>
        </recommendedName>
        <alternativeName>
            <fullName>Cation efflux system protein CzcB</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">czcB</name>
        <name type="ordered locus">Rmet_5981</name>
    </gene>
    <organism>
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid">
        <name>pMOL30</name>
    </geneLocation>
    <reference key="1">
        <citation type="journal article" date="1989" name="Proc. Natl. Acad. Sci. U.S.A." volume="86" first="7351" last="7355">
            <title>Expression and nucleotide sequence of a plasmid-determined divalent cation efflux system from Alcaligenes eutrophus.</title>
            <authorList>
                <person name="Nies D.H."/>
                <person name="Nies A."/>
                <person name="Chu L."/>
                <person name="Silver S."/>
            </authorList>
            <dbReference type="PubMed" id="2678100"/>
            <dbReference type="DOI" id="10.1073/pnas.86.19.7351"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC DNA]</scope>
    </reference>
    <reference key="2">
        <citation type="submission" date="1996-06" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="van der Lelie D."/>
                <person name="Schwuchow T."/>
                <person name="Wuertz S."/>
                <person name="Schwidetzky U."/>
                <person name="Baeyens W."/>
                <person name="Scheel P.O."/>
                <person name="Nies D.H."/>
            </authorList>
        </citation>
        <scope>SEQUENCE REVISION</scope>
    </reference>
    <reference key="3">
        <citation type="submission" date="2004-11" db="EMBL/GenBank/DDBJ databases">
            <title>Sequence and features of the Ralstonia metallidurans CH34 heavy metals plasmids pMOL28 and pMOL30.</title>
            <authorList>
                <person name="Monchy S."/>
                <person name="van der Lelie D."/>
                <person name="Vallaeys T."/>
                <person name="Taghavi S."/>
                <person name="Benotmane M."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="Lapidus A."/>
                <person name="Mergeay M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference key="4">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <reference key="5">
        <citation type="journal article" date="1995" name="J. Ind. Microbiol." volume="14" first="142" last="153">
            <title>The czc operon of Alcaligenes eutrophus CH34: from resistance mechanism to the removal of heavy metals.</title>
            <authorList>
                <person name="Diels L."/>
                <person name="Dong Q."/>
                <person name="van der Lelie D."/>
                <person name="Baeyens W."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="7766206"/>
            <dbReference type="DOI" id="10.1007/BF01569896"/>
        </citation>
        <scope>CHARACTERIZATION</scope>
        <scope>INDUCTION</scope>
        <scope>POSSIBLE MODE OF ACTION</scope>
    </reference>
    <comment type="function">
        <text>CzcA and CzcB together would act in zinc efflux nearly as effectively as the complete czc efflux system (CzcABC). The CzcB protein is thought to funnel zinc cations to the CzcA transport protein.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cell inner membrane</location>
            <topology evidence="4">Single-pass membrane protein</topology>
        </subcellularLocation>
        <text>May be anchored in the inner membrane, cross the periplasm and contact the outer membrane as proposed for membrane fusion proteins.</text>
    </comment>
    <comment type="induction">
        <text evidence="3">By zinc, cadmium and cobalt (zinc being the best and cobalt being the worst inducer).</text>
    </comment>
    <comment type="biotechnology">
        <text evidence="3">In the presence of 2 mM Cd(2+) or 4-10 mM Zn(2+) up to 99% of the metal is removed from the culture supernatant and is sequestered via bioprecipitation. Additionally Cu(2+), Co(2+), Ni(2+), Pb(2+), Y(3+) and Ge(4+) can also be removed from culture supernatants, although it is not clear which efflux system is responsible for all these substrates (PubMed:7766206).</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family.</text>
    </comment>
    <dbReference type="EMBL" id="X98451">
        <property type="protein sequence ID" value="CAA67083.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="X71400">
        <property type="protein sequence ID" value="CAI11243.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP000354">
        <property type="protein sequence ID" value="ABF12840.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B33830">
        <property type="entry name" value="B33830"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004635340.1">
        <property type="nucleotide sequence ID" value="NC_007971.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="YP_145594.1">
        <property type="nucleotide sequence ID" value="NC_006466.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="P13510"/>
    <dbReference type="SMR" id="P13510"/>
    <dbReference type="TCDB" id="2.A.6.1.2">
        <property type="family name" value="the resistance-nodulation-cell division (rnd) superfamily"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF12840">
        <property type="protein sequence ID" value="ABF12840"/>
        <property type="gene ID" value="Rmet_5981"/>
    </dbReference>
    <dbReference type="GeneID" id="60820445"/>
    <dbReference type="KEGG" id="rme:Rmet_5981"/>
    <dbReference type="HOGENOM" id="CLU_018816_13_0_4"/>
    <dbReference type="OMA" id="VIPKREY"/>
    <dbReference type="OrthoDB" id="1174564at2"/>
    <dbReference type="PRO" id="PR:P13510"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid pMOL30"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046873">
        <property type="term" value="F:metal ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046686">
        <property type="term" value="P:response to cadmium ion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005695">
        <property type="entry name" value="Co/Zn/Cd_resistance_CzcB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043602">
        <property type="entry name" value="CusB_dom_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032317">
        <property type="entry name" value="HlyD_D23"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006143">
        <property type="entry name" value="RND_pump_MFP"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00529">
        <property type="entry name" value="CusB_dom_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16576">
        <property type="entry name" value="HlyD_D23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00999">
        <property type="entry name" value="8a0102"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01730">
        <property type="entry name" value="RND_mfp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0105">Cadmium resistance</keyword>
    <keyword id="KW-0997">Cell inner membrane</keyword>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0170">Cobalt</keyword>
    <keyword id="KW-0175">Coiled coil</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-0614">Plasmid</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <keyword id="KW-0813">Transport</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <feature type="chain" id="PRO_0000201867" description="Cobalt-zinc-cadmium resistance protein CzcB">
        <location>
            <begin position="1"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="9"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="28"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="286"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="36"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="sequence conflict" description="In Ref. 1; CAA67083." evidence="4" ref="1">
        <original>D</original>
        <variation>A</variation>
        <location>
            <position position="338"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="3">
        <source>
            <dbReference type="PubMed" id="7766206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="4"/>
    <sequence length="520" mass="54631" checksum="0E4A460A887F79A4" modified="2006-07-11" version="2"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="63" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LA05</accession>
    <name>Q1LA05_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transcriptional regulator XRE family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_6162</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2 3">
        <name>pMOL30</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid pMOL30</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000354">
        <property type="protein sequence ID" value="ABF13021.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011514827.1">
        <property type="nucleotide sequence ID" value="NC_007971.2"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF13021">
        <property type="protein sequence ID" value="ABF13021"/>
        <property type="gene ID" value="Rmet_6162"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6162"/>
    <dbReference type="HOGENOM" id="CLU_113623_0_0_4"/>
    <dbReference type="OMA" id="IYWNSMT"/>
    <dbReference type="OrthoDB" id="1819195at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid pMOL30"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00093">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001387">
        <property type="entry name" value="Cro/C1-type_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010982">
        <property type="entry name" value="Lambda_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47413">
        <property type="entry name" value="SSF47413"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF13021.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="207" mass="23109" checksum="67D8E56BA5F5FDBB" modified="2006-05-30" version="1">MTKSAPAHGGGSHAAAPTRRVSAKSRKTTEVTAKMTTHEQHGAESTASEHEAEASGKRHVDGIRLINLIRKTLIDRGLPQRTISDILGVTPVYWNSIMSGNRSIKSLGKDRFDTIAEWLGIPTVQVLNQADYLSIEDFYSKKDLHEQLWLAIVKMANDPQWMAYAPTREEWDVLPLRVQIGYAALYERDYGRVLTAKAQIEVPDLVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="53" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LA46</accession>
    <name>Q1LA46_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Protein involved in Cu(II)/Cu(I) resistance(Secreted)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">copQ</name>
        <name evidence="3" type="ordered locus">Rmet_6121</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3 4">
        <name>pMOL30</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid pMOL30</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000354">
        <property type="protein sequence ID" value="ABF12980.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008652083.1">
        <property type="nucleotide sequence ID" value="NC_007971.2"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF12980">
        <property type="protein sequence ID" value="ABF12980"/>
        <property type="gene ID" value="Rmet_6121"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6121"/>
    <dbReference type="HOGENOM" id="CLU_136209_5_1_4"/>
    <dbReference type="OMA" id="DGAHAMG"/>
    <dbReference type="OrthoDB" id="940209at1224"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid pMOL30"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004192961" evidence="2">
        <location>
            <begin position="27"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="22"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF12980.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="78" mass="7865" checksum="19A0AE240A624A4F" modified="2006-05-30" version="1">MKTLKKSVLLVLAVATLGGTAGVANAGVTSPRDPYTDGGHSMGGRDPFTDGAHSMGTRDPFTDGGHSIGTRDTFSDGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LAY3</accession>
    <name>Q1LAY3_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">acidPPc domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_5834</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12693.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518043.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5834"/>
    <dbReference type="EnsemblBacteria" id="ABF12693">
        <property type="protein sequence ID" value="ABF12693"/>
        <property type="gene ID" value="Rmet_5834"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5834"/>
    <dbReference type="eggNOG" id="COG0671">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_079852_2_0_4"/>
    <dbReference type="OMA" id="AMSATLW"/>
    <dbReference type="OrthoDB" id="1525433at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036938">
        <property type="entry name" value="P_Acid_Pase_2/haloperoxi_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000326">
        <property type="entry name" value="P_Acid_Pase_2/haloperoxidase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01569">
        <property type="entry name" value="PAP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48317">
        <property type="entry name" value="SSF48317"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="39"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="72"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="98"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="156"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="domain" description="acidPPc" evidence="2">
        <location>
            <begin position="72"/>
            <end position="140"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01569"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF12693.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="226" mass="24229" checksum="6B18072556DB1A35" modified="2006-05-30" version="1">MSRWQLVSFFGESAFLLPCAVFLYAWLRWHGANAVARHWLIAFSLTACLVLVSKLAFMGWGIGSAALNFTGFSGHAMMAASILPVFACLAVPSRYRTLSLLAAVAGVLLALAVGMSRLALHAHSMSEVVSGLALGFCVSLPFIMRHEVPHGPVAMLLASGVLATVLVLPVSGVAGVTHIWVQGIATFLSGRDRPYERGEWAWQADCRVCQINVAARPDCATRRCAC</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="102" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LB16</accession>
    <name>Q1LB16_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Glucose-6-phosphate 1-dehydrogenase</fullName>
            <shortName evidence="5">G6PD</shortName>
            <ecNumber evidence="5">1.1.1.49</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5 9" type="primary">zwf</name>
        <name evidence="9" type="ordered locus">Rmet_5801</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="10">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="10">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Catalyzes the oxidation of glucose 6-phosphate to 6-phosphogluconolactone.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>D-glucose 6-phosphate + NADP(+) = 6-phospho-D-glucono-1,5-lactone + H(+) + NADPH</text>
            <dbReference type="Rhea" id="RHEA:15841"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:57955"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:61548"/>
            <dbReference type="EC" id="1.1.1.49"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="5">Carbohydrate degradation; pentose phosphate pathway; D-ribulose 5-phosphate from D-glucose 6-phosphate (oxidative stage): step 1/3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the glucose-6-phosphate dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.49" evidence="5"/>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12660.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5801"/>
    <dbReference type="EnsemblBacteria" id="ABF12660">
        <property type="protein sequence ID" value="ABF12660"/>
        <property type="gene ID" value="Rmet_5801"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5801"/>
    <dbReference type="eggNOG" id="COG0364">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013524_5_0_4"/>
    <dbReference type="OMA" id="PDEGIQM"/>
    <dbReference type="UniPathway" id="UPA00115">
        <property type="reaction ID" value="UER00408"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004345">
        <property type="term" value="F:glucose-6-phosphate dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006006">
        <property type="term" value="P:glucose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006098">
        <property type="term" value="P:pentose-phosphate shunt"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00966">
        <property type="entry name" value="G6PD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001282">
        <property type="entry name" value="G6P_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019796">
        <property type="entry name" value="G6P_DH_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022675">
        <property type="entry name" value="G6P_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022674">
        <property type="entry name" value="G6P_DH_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23429">
        <property type="entry name" value="PTHR23429"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02781">
        <property type="entry name" value="G6PD_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00479">
        <property type="entry name" value="G6PD_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000110">
        <property type="entry name" value="G6PD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00079">
        <property type="entry name" value="G6PDHDRGNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00871">
        <property type="entry name" value="zwf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00069">
        <property type="entry name" value="G6P_DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="1 5" id="KW-0313">Glucose metabolism</keyword>
    <keyword evidence="2 5" id="KW-0521">NADP</keyword>
    <keyword evidence="3 5 9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-0614">Plasmid</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="G6PD_N" evidence="7">
        <location>
            <begin position="21"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="domain" description="G6PD_C" evidence="8">
        <location>
            <begin position="196"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="5">
        <location>
            <position position="247"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="24"/>
            <end position="31"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="58"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="155"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="185"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5 6">
        <location>
            <position position="189"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="223"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="242"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="347"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022526"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ABF12660.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="496" mass="55906" checksum="DE0A30F126ED2DF7" modified="2006-05-30" version="1">MPGRPIPRKEPRVSLPDFDMVLFGGTGDLARRKLLPALFDAHRTGCLHPSSRILATGSHPHTTEQYKAMLEESVRPGLEHAPPEAWTSFMDRIGYVQADARQPAHFDALAEQVLARKPEVVVCYLATAPHIFVPICEQLGRTGLNAPNVRVVLEKPLGHDLDSSEAINNAVSRYFGEEQIYRIDHYLGKESVQNLMAIRFANALFEPLWRREWVQDVQITIAEELGVETRGDFYDRTGALRDMVQNHLVQLLCMVAMEPPASLSEDAIRDEKIKILKALKPILPQDVAEKTVRGQYRAGAVGGKPVVSYVDEQGIAADSTTETFVAIKAEIANWRWEGVPFYLRTGKRMQSRVAEIVVHFRDVPHAIFPRPLTLSPQNRLVIRLQPEESIRLYFLVKQPGDTVELTPTSLDLDFANSFKMRRAGAYERLLLDVIRGKLGLFVRRDEQVQAWRWVEPILDTWDQSTVPPKPYTAGTWGPAASSALMSRDGALWHEEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="61" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LB22</accession>
    <name>Q1LB22_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Oligopeptide transporter, OPT superfamily</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Rmet_5795</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="8">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="8">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12654.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5795"/>
    <dbReference type="EnsemblBacteria" id="ABF12654">
        <property type="protein sequence ID" value="ABF12654"/>
        <property type="gene ID" value="Rmet_5795"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5795"/>
    <dbReference type="eggNOG" id="COG1297">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_440635_0_0_4"/>
    <dbReference type="OMA" id="CDMQQDR"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035673">
        <property type="term" value="F:oligopeptide transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004813">
        <property type="entry name" value="OPT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045035">
        <property type="entry name" value="YSL-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31645">
        <property type="entry name" value="PTHR31645"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03169">
        <property type="entry name" value="OPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0614">Plasmid</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="71"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="98"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="131"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="161"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="217"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="248"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="290"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="332"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="357"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="441"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="509"/>
            <end position="528"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="567"/>
            <end position="586"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="606"/>
            <end position="633"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABF12654.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="634" mass="66069" checksum="5242FB11A6D8CF95" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="122" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LB93</accession>
    <name>Q1LB93_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Histidine kinase</fullName>
            <ecNumber evidence="2">2.7.13.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">bvgS2</name>
        <name evidence="9" type="ordered locus">Rmet_5724</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="10">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="10">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.</text>
            <dbReference type="EC" id="2.7.13.3"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.7.13.3" evidence="2"/>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12583.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011520124.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5724"/>
    <dbReference type="EnsemblBacteria" id="ABF12583">
        <property type="protein sequence ID" value="ABF12583"/>
        <property type="gene ID" value="Rmet_5724"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5724"/>
    <dbReference type="eggNOG" id="COG2205">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_114_15_4"/>
    <dbReference type="OMA" id="THERILP"/>
    <dbReference type="OrthoDB" id="1755994at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00082">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00130">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003661">
        <property type="entry name" value="HisK_dim/P"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036097">
        <property type="entry name" value="HisK_dim/P_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000014">
        <property type="entry name" value="PAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035965">
        <property type="entry name" value="PAS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013767">
        <property type="entry name" value="PAS_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004358">
        <property type="entry name" value="Sig_transdc_His_kin-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00512">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00989">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00344">
        <property type="entry name" value="BCTRLSENSOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00388">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00091">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47384">
        <property type="entry name" value="SSF47384"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55785">
        <property type="entry name" value="SSF55785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="SSF55874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00229">
        <property type="entry name" value="sensory_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50112">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9" id="KW-0614">Plasmid</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="6"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="domain" description="PAS" evidence="8">
        <location>
            <begin position="43"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="6">
        <location>
            <begin position="317"/>
            <end position="538"/>
        </location>
    </feature>
    <feature type="domain" description="Response regulatory" evidence="7">
        <location>
            <begin position="665"/>
            <end position="782"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="4">
        <location>
            <position position="714"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ABF12583.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="784" mass="85743" checksum="CF25EE6FEEB6DA8A" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="111" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LBA3</accession>
    <name>Q1LBA3_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Two component transcriptional regulator, LuxR family (Receiver domain) (BvgA-like)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">bvgA</name>
        <name evidence="5" type="ordered locus">Rmet_5714</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="6">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="6">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12573.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011520117.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5714"/>
    <dbReference type="EnsemblBacteria" id="ABF12573">
        <property type="protein sequence ID" value="ABF12573"/>
        <property type="gene ID" value="Rmet_5714"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5714"/>
    <dbReference type="eggNOG" id="COG2197">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_90_1_4"/>
    <dbReference type="OMA" id="PHVLTHM"/>
    <dbReference type="OrthoDB" id="1687028at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06170">
        <property type="entry name" value="LuxR_C_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016032">
        <property type="entry name" value="Sig_transdc_resp-reg_C-effctor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000792">
        <property type="entry name" value="Tscrpt_reg_LuxR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00196">
        <property type="entry name" value="GerE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00038">
        <property type="entry name" value="HTHLUXR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00421">
        <property type="entry name" value="HTH_LUXR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46894">
        <property type="entry name" value="SSF46894"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00622">
        <property type="entry name" value="HTH_LUXR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50043">
        <property type="entry name" value="HTH_LUXR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="5" id="KW-0614">Plasmid</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Response regulatory" evidence="4">
        <location>
            <begin position="3"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="domain" description="HTH luxR-type" evidence="3">
        <location>
            <begin position="142"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="2">
        <location>
            <position position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABF12573.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="210" mass="23018" checksum="167873FC0E8CD9B1" modified="2006-05-30" version="1">MITVLIVDDHPAVRLVIKTQLSQVLGVSSVLEADNGQAAIDVVRQCKPDLVILDLDIPKINGLEVIPRLKAIQPNIRVLVISGQDPMTFAPRAMQVGAKGFVSKMQDMTDIMRCVESVLAGYTLFPAEVVESGAVPGKAREDSELLQQLTDKEIQIMQMLSKGMSNKAIGDSLFISNKTVSSHKTRIMEKLHVTTLLELIDFARRCNIAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="67" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LBF5</accession>
    <name>Q1LBF5_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_5662</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12521.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011520066.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5662"/>
    <dbReference type="EnsemblBacteria" id="ABF12521">
        <property type="protein sequence ID" value="ABF12521"/>
        <property type="gene ID" value="Rmet_5662"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5662"/>
    <dbReference type="eggNOG" id="COG1917">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_174136_0_0_4"/>
    <dbReference type="OMA" id="ADHWCKK"/>
    <dbReference type="OrthoDB" id="1745958at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011051">
        <property type="entry name" value="RmlC_Cupin_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51182">
        <property type="entry name" value="SSF51182"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF12521.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="113" mass="12975" checksum="23FBFFBA3B7396DE" modified="2006-05-30" version="1">MKMDAFPFGTTDWSQIERTEHKGDTGAAYWRTQYFGEGDRRVRVRMVEYSPGYLADHWCKKGHVLFCLQGELETTLEDGRQFMLRPGMSYQVGDDAEAHQSRTSTGATLFIVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="66" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LBH8</accession>
    <name>Q1LBH8_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_5639</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12498.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011520042.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5639"/>
    <dbReference type="EnsemblBacteria" id="ABF12498">
        <property type="protein sequence ID" value="ABF12498"/>
        <property type="gene ID" value="Rmet_5639"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5639"/>
    <dbReference type="eggNOG" id="COG3656">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_129911_0_0_4"/>
    <dbReference type="OMA" id="EGTKWLK"/>
    <dbReference type="OrthoDB" id="2021063at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014469">
        <property type="entry name" value="DUF2271"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10029">
        <property type="entry name" value="DUF2271"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF014995">
        <property type="entry name" value="UCP014995"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193276" evidence="2">
        <location>
            <begin position="23"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="153"/>
            <end position="173"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF12498.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="173" mass="18857" checksum="B08C006DBA0DCE25" modified="2006-05-30" version="1">MRRLLTVTVTGLAAAPLAPAMAADMNVKVEIPRLNVAEYHRPYVSMWIERADQSPVTTLAVWYDAKNKESQGTKWLKDMRQWWRKTGRDMQLPADGVSGATRAPGEHALTFSDGKAPLGKLPAGDYQLVVEAAREVGGRELVRVPFAWPPKAAQSAGAKGEHELGGVSVDLKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="67" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LBW1</accession>
    <name>Q1LBW1_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_5506</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12365.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519908.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5506"/>
    <dbReference type="EnsemblBacteria" id="ABF12365">
        <property type="protein sequence ID" value="ABF12365"/>
        <property type="gene ID" value="Rmet_5506"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5506"/>
    <dbReference type="eggNOG" id="COG1917">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1508649_0_0_4"/>
    <dbReference type="OMA" id="NADCLYY"/>
    <dbReference type="OrthoDB" id="1928636at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011051">
        <property type="entry name" value="RmlC_Cupin_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51182">
        <property type="entry name" value="SSF51182"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF12365.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="184" mass="20443" checksum="C4AE284B0C892F04" modified="2006-05-30" version="1">MATEKTSTSSSEHRRPKRIQFFHAADAQDLLESGAMHFKPLPPERAELNPVFAQGVKEGSVSRLLFQAENGFSLVYSWFKSGFRLPAHSHDTDCLYYVISGEIRYGTQVLGCGDGFFVPAHTVYGYTPGPQGVEILEFRSVSEFDIELISAPMSAWERAAHMVQSSLERWRSENPPARASSGLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="108" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LCE5</accession>
    <name>Q1LCE5_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Histidine kinase</fullName>
            <ecNumber evidence="2">2.7.13.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">zniS</name>
        <name evidence="10" type="ordered locus">Rmet_5322</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="11">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="11">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.</text>
            <dbReference type="EC" id="2.7.13.3"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.7.13.3" evidence="2"/>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12181.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5322"/>
    <dbReference type="DNASU" id="4042183"/>
    <dbReference type="EnsemblBacteria" id="ABF12181">
        <property type="protein sequence ID" value="ABF12181"/>
        <property type="gene ID" value="Rmet_5322"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5322"/>
    <dbReference type="eggNOG" id="COG2205">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_508858_0_0_4"/>
    <dbReference type="OMA" id="SSWRIHQ"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00082">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003660">
        <property type="entry name" value="HAMP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003661">
        <property type="entry name" value="HisK_dim/P"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036097">
        <property type="entry name" value="HisK_dim/P_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024478">
        <property type="entry name" value="HlyB_4HB_MCP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004358">
        <property type="entry name" value="Sig_transdc_His_kin-like_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12729">
        <property type="entry name" value="4HB_MCP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00672">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00512">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00344">
        <property type="entry name" value="BCTRLSENSOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00304">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00388">
        <property type="entry name" value="HisKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47384">
        <property type="entry name" value="SSF47384"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="SSF55874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50885">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5 10" id="KW-0418">Kinase</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="10" id="KW-0614">Plasmid</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="20"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="198"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="domain" description="HAMP" evidence="9">
        <location>
            <begin position="219"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="8">
        <location>
            <begin position="304"/>
            <end position="522"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="88"/>
            <end position="115"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ABF12181.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="529" mass="58264" checksum="6706AD786F92766A" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LCK2</accession>
    <name>Q1LCK2_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Flagellar FliJ protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">fliJ</name>
        <name evidence="13" type="ordered locus">Rmet_5265</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="14">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="14">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
            <orientation evidence="2">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the FliJ family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12124.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519670.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5265"/>
    <dbReference type="EnsemblBacteria" id="ABF12124">
        <property type="protein sequence ID" value="ABF12124"/>
        <property type="gene ID" value="Rmet_5265"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5265"/>
    <dbReference type="eggNOG" id="COG2882">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_119965_2_0_4"/>
    <dbReference type="OMA" id="KRDQKLM"/>
    <dbReference type="OrthoDB" id="2042666at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009288">
        <property type="term" value="C:bacterial-type flagellum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003774">
        <property type="term" value="F:cytoskeletal motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044781">
        <property type="term" value="P:bacterial-type flagellum organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071973">
        <property type="term" value="P:bacterial-type flagellum-dependent cell motility"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006935">
        <property type="term" value="P:chemotaxis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018006">
        <property type="entry name" value="Flag_FliJ_proteobac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012823">
        <property type="entry name" value="Flagell_FliJ"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02050">
        <property type="entry name" value="FliJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF019404">
        <property type="entry name" value="FliJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01004">
        <property type="entry name" value="FLGFLIJ"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02473">
        <property type="entry name" value="flagell_FliJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1005">Bacterial flagellum biogenesis</keyword>
    <keyword evidence="6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="13" id="KW-0966">Cell projection</keyword>
    <keyword evidence="7" id="KW-0145">Chemotaxis</keyword>
    <keyword evidence="13" id="KW-0969">Cilium</keyword>
    <keyword evidence="11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="13" id="KW-0282">Flagellum</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-0614">Plasmid</keyword>
    <keyword evidence="9" id="KW-0653">Protein transport</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="115"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="28"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="122"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004287"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004413"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00020392"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022500"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022795"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ABF12124.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="150" mass="17234" checksum="F3D0A54E89FFFDF3" modified="2006-05-30" version="1">MVKHNPLNTLADLAQDDTDKAARELGRLQGLRTQAEQQLNALTQYRQEYRIRMQAIAAEGMSSSRWHDFSRFLDSLDHAIRQQTNALAKAEADLLAGRANWQQQKRRQNSFDTLITRAETREQQVSARREQRANDEYAARIARQPAAGRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LCZ8</accession>
    <name>Q1LCZ8_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Taurine catabolism dioxygenase TauD/TfdA (2-oxoglutarate-dependent)</fullName>
            <ecNumber evidence="3">1.14.11.17</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_5119</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EC" id="1.14.11.17" evidence="3"/>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11978.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519525.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5119"/>
    <dbReference type="EnsemblBacteria" id="ABF11978">
        <property type="protein sequence ID" value="ABF11978"/>
        <property type="gene ID" value="Rmet_5119"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5119"/>
    <dbReference type="eggNOG" id="COG2175">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036005_2_1_4"/>
    <dbReference type="OMA" id="WANTEAA"/>
    <dbReference type="OrthoDB" id="1742732at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000908">
        <property type="term" value="F:taurine dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.130.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042098">
        <property type="entry name" value="TauD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003819">
        <property type="entry name" value="TauD/TfdA-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02668">
        <property type="entry name" value="TauD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="1 3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TauD" evidence="2">
        <location>
            <begin position="3"/>
            <end position="274"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF11978.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="299" mass="33690" checksum="02AD5CD3DEBB8F63" modified="2006-05-30" version="1">MRIEPMTASIGAELVGVRLSDAIHDDGLFDEIRAALLRHKVLFLRDQDISRAEHVAFAERFGKLEDHPVAGSHPDHPGLVQIYKRPDQPMDRYENAWHTDATWREAPPLGCVLRCVESPEIGGDTMWANMVLAYENLPAHVKTQIAGLRARHSIEASFGAAMPIEKRLALKAQYPDPEHPVVRVHPETGEKVLFVNAFTTHFSNFHTPAHVRYGQDYNPGAGELLRYLISQAFIPEYQVRWRWKANSMAIWDNRSTQHYAVMDYAPCHRKMERAGIIGDKPFGIGEGEVKAESAQPATA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="104" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LD27</accession>
    <name>Q1LD27_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">ABC transporter permease protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">dppC</name>
        <name evidence="7" type="ordered locus">Rmet_5090</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="8">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="8">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cell membrane</location>
            <topology evidence="5">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the binding-protein-dependent transport system permease family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11949.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519495.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5090"/>
    <dbReference type="EnsemblBacteria" id="ABF11949">
        <property type="protein sequence ID" value="ABF11949"/>
        <property type="gene ID" value="Rmet_5090"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5090"/>
    <dbReference type="eggNOG" id="COG1173">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028518_1_1_4"/>
    <dbReference type="OMA" id="FIRSVYI"/>
    <dbReference type="OrthoDB" id="1734230at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025966">
        <property type="entry name" value="OppC_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12911">
        <property type="entry name" value="OppC_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="SSF161098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0614">Plasmid</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="36"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="100"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="144"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="216"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="258"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="6">
        <location>
            <begin position="100"/>
            <end position="284"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABF11949.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="299" mass="31785" checksum="741E4FEBC89351E3" modified="2006-05-30" version="1">MTATLPSRQDAAARPADDDAPFVLPRWYWARKHPTLVIGAVLLLAVAALAIAAPWISPFDPQDIDPLARMQPPSAEHWFGTDALGRDVFSRAVWGGRVSMIVALSVGLLSTVLGVSIGLTAGFVRWTDGFLMRVMDGLMAIPGILLAIALMALTQASLGAVIVAITVPEVPRVVRLVRSMALTLREQLYVEAAHAVGTRLPVILLRHVLPNMVAPLIVQATFVAAAAVLTEAALSFLGVGVPAQTPSWGNIMAEGRNFVAVAFHILLYPGLLLAATVLAINLLGDGLRDALDPRLARQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LD89</accession>
    <name>Q1LD89_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Acetyl-CoA/propanoyl-CoA C-acyltransferase (Thiolase)</fullName>
            <ecNumber evidence="6">2.3.1.16</ecNumber>
            <ecNumber evidence="6">2.3.1.176</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Rmet_5025</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="7">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="7">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the thiolase-like superfamily. Thiolase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.16" evidence="6"/>
    <dbReference type="EC" id="2.3.1.176" evidence="6"/>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11887.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519436.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5025"/>
    <dbReference type="EnsemblBacteria" id="ABF11887">
        <property type="protein sequence ID" value="ABF11887"/>
        <property type="gene ID" value="Rmet_5025"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5025"/>
    <dbReference type="eggNOG" id="COG0183">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035425_0_0_4"/>
    <dbReference type="OMA" id="PSLYAMM"/>
    <dbReference type="OrthoDB" id="311458at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003988">
        <property type="term" value="F:acetyl-CoA C-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033814">
        <property type="term" value="F:propanoyl-CoA C-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050632">
        <property type="term" value="F:propionyl-CoA C2-trimethyltridecanoyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.47.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002155">
        <property type="entry name" value="Thiolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016039">
        <property type="entry name" value="Thiolase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020615">
        <property type="entry name" value="Thiolase_acyl_enz_int_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020617">
        <property type="entry name" value="Thiolase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020613">
        <property type="entry name" value="Thiolase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020616">
        <property type="entry name" value="Thiolase_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02803">
        <property type="entry name" value="Thiolase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00108">
        <property type="entry name" value="Thiolase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000429">
        <property type="entry name" value="Ac-CoA_Ac_transf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53901">
        <property type="entry name" value="SSF53901"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00098">
        <property type="entry name" value="THIOLASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00737">
        <property type="entry name" value="THIOLASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="6" id="KW-0614">Plasmid</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3 6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Thiolase_N" evidence="4">
        <location>
            <begin position="5"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="domain" description="Thiolase_C" evidence="5">
        <location>
            <begin position="274"/>
            <end position="376"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF02803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABF11887.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="395" mass="41937" checksum="A57EF508DECF2977" modified="2006-05-30" version="1">MTRKVFVAGAGMIPFKKPGTSDTYDVMGATATRQALADAGVDYNLVQQAYVGYVYGDSTCGQKALYDVGMSGIPVINVNNNCATGSSALFLARQAVASGAVECALALGFEFMGPGALKSVWTDRPSALERALNLTDKLVGKTEGMSNAIRQFAGAGQTHMRKHGTKLETFAKIRAKASRHAANNPLAVFRTVVSTEDVMNAPMLWDGVLTRLMACPPTCGAAAAIVVSEAFARKHGLRTDVLIAAQSLTTDRASSFESDMIRLVGFDMTREGARQVYEQASIGPQDVDVIELHDCFAQNELLTYEGLGLCDEGCAEKLVNDGDNTYGGKWVVNPSGGLLSKGHPLGATGLAQCYEMVQQLRGNADQRQVAGARNALTHNLGLGGACVVTLYQKHD</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="97" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LD99</accession>
    <name>Q1LD99_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Choline dehydrogenase, a flavoprotein</fullName>
            <ecNumber evidence="7">1.1.99.1</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">betA3</name>
        <name evidence="7" type="ordered locus">Rmet_5015</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="8">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="8">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the GMC oxidoreductase family.</text>
    </comment>
    <dbReference type="EC" id="1.1.99.1" evidence="7"/>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11877.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519426.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5015"/>
    <dbReference type="EnsemblBacteria" id="ABF11877">
        <property type="protein sequence ID" value="ABF11877"/>
        <property type="gene ID" value="Rmet_5015"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5015"/>
    <dbReference type="eggNOG" id="COG2303">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002865_7_1_4"/>
    <dbReference type="OMA" id="WYPGGPT"/>
    <dbReference type="OrthoDB" id="543793at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008812">
        <property type="term" value="F:choline dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012132">
        <property type="entry name" value="GMC_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000172">
        <property type="entry name" value="GMC_OxRdtase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007867">
        <property type="entry name" value="GMC_OxRtase_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11552">
        <property type="entry name" value="PTHR11552"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05199">
        <property type="entry name" value="GMC_oxred_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00732">
        <property type="entry name" value="GMC_oxred_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000137">
        <property type="entry name" value="Alcohol_oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="SSF51905"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00623">
        <property type="entry name" value="GMC_OXRED_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00624">
        <property type="entry name" value="GMC_OXRED_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0274">FAD</keyword>
    <keyword evidence="3" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-0614">Plasmid</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GMC_OxRdtase_N" evidence="5">
        <location>
            <begin position="95"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="domain" description="GMC_OxRdtase_N" evidence="6">
        <location>
            <begin position="275"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="78"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="105"/>
            <end position="108"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="240"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010790"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR000137-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS00623"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS00624"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABF11877.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="557" mass="60321" checksum="D80CCE8587D899D4" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="111" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LDA3</accession>
    <name>Q1LDA3_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Pyridine nucleotide-disulfide oxidoreductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ordered locus">Rmet_5011</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="13">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="13">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="7">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
        <text evidence="7">Binds 1 FAD per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 9">Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11873.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519422.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5011"/>
    <dbReference type="EnsemblBacteria" id="ABF11873">
        <property type="protein sequence ID" value="ABF11873"/>
        <property type="gene ID" value="Rmet_5011"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5011"/>
    <dbReference type="eggNOG" id="COG1249">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016755_1_2_4"/>
    <dbReference type="OMA" id="RIAAYAM"/>
    <dbReference type="OrthoDB" id="267896at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016668">
        <property type="term" value="F:oxidoreductase activity, acting on a sulfur group of donors, NAD(P) as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045454">
        <property type="term" value="P:cell redox homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.390.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023753">
        <property type="entry name" value="FAD/NAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016156">
        <property type="entry name" value="FAD/NAD-linked_Rdtase_dimer_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001100">
        <property type="entry name" value="Pyr_nuc-diS_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004099">
        <property type="entry name" value="Pyr_nucl-diS_OxRdtase_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012999">
        <property type="entry name" value="Pyr_OxRdtase_I_AS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07992">
        <property type="entry name" value="Pyr_redox_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02852">
        <property type="entry name" value="Pyr_redox_dim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000350">
        <property type="entry name" value="Mercury_reductase_MerA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="SSF51905"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55424">
        <property type="entry name" value="SSF55424"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00076">
        <property type="entry name" value="PYRIDINE_REDOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7 9" id="KW-0274">FAD</keyword>
    <keyword evidence="2 9" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="7" id="KW-0520">NAD</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4 9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="12" id="KW-0614">Plasmid</keyword>
    <keyword evidence="5 9" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pyr_redox_2" evidence="11">
        <location>
            <begin position="6"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="domain" description="Pyr_redox_dim" evidence="10">
        <location>
            <begin position="345"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="6">
        <location>
            <position position="441"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="51"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="177"/>
            <end position="184"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="200"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="267"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="308"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="disulfide bond" description="Redox-active" evidence="8">
        <location>
            <begin position="42"/>
            <end position="47"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR000350-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR000350-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR000350-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF02852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF07992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ABF11873.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="459" mass="49333" checksum="B18F13FFE500687D" modified="2006-05-30" version="1">MAQRFDAIIIGTGQAGPALAARLAGAGMKLAVIERGRFGGTCVNTGCIPTKALIASAYAAQLARRAAEYGVTIGGPVAVDMRQVKARKDEISGRSSQGVEQWMRGLDNVTVHQGHARFEHARGVRVNGELLEADQIFINVGGRALIPPMPGLDQVSYLTNSSMMEVDFLPEHLIIVGGSYIGLEFGQMYRRFGARVTIVEKGPRLIQREDEDVSQAVREILEREGIEVRLDANCLSARKDGDHIAVGLDCAGGAPEVHGSHLLMAVGRVPNTDDLGLDKAGIETDARGYIKVDDQLRTSVPGIWALGDCNGRGAFTHTSYNDYEIVAANLLDNDPRAVTDRIPAYALFTDPPLGRIGMTEREARQSGRKVLVGTRPMSRVGRAVEKGESQGFMKILVDADTRLLLGAAILGLNGDEVVHSLLDVMYAGAPYTTISRAVHIHPTVSELVPTVLQGLRALT</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="101" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LDG0</accession>
    <name>Q1LDG0_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Transcriptional regulator, GntR family, aminotransferase related</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_4954</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">In the C-terminal section; belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11816.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519375.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4954"/>
    <dbReference type="EnsemblBacteria" id="ABF11816">
        <property type="protein sequence ID" value="ABF11816"/>
        <property type="gene ID" value="Rmet_4954"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4954"/>
    <dbReference type="eggNOG" id="COG1167">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017584_0_0_4"/>
    <dbReference type="OMA" id="NMVFPRH"/>
    <dbReference type="OrthoDB" id="1024978at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008483">
        <property type="term" value="F:transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009058">
        <property type="term" value="P:biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07377">
        <property type="entry name" value="WHTH_GntR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000524">
        <property type="entry name" value="Tscrpt_reg_HTH_GntR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00392">
        <property type="entry name" value="GntR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00345">
        <property type="entry name" value="HTH_GNTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="SSF53383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50949">
        <property type="entry name" value="HTH_GNTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="HTH gntR-type" evidence="2">
        <location>
            <begin position="21"/>
            <end position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF11816.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="466" mass="50920" checksum="433D5DE743CEFDD7" modified="2006-05-30" version="1">MSDMKGERHVEWLRPLTPGAGPRYMQIADLIARAVQTGELAVGDQIPSQRWLATQLGVDLTTVTRAYTEARNRGLISSFSGRGSFVAGVGEAGDEMRIDLSMNTPPQPASGSMGDALKLAIDEVLARQGIEAMSAYQDTWSGASIVQAGRAWLRPAVGAMAGQQELIVCAGTQAAIFSLLQSRTQRGDAVLAEPLTYPGFLLTAQQLGLRVVALEVDQDGVLPDAIERSHRETGAQVIYLNPTLQNPTASTMPEHRREAIGAMLRKLGMTLIEDDPYRYLLNDAPPPIVTYEGGERAYYLTSLSKCLWPSLRTSFVLLPRQHDAAMLQDALRASSMGCSLLLTAVAEHWMRSGQARHLMLDIQREVRARQTLARNLLPKDVNAHPTGLHLWMTLPAHWNRQLFALALEQQGVLVACSDAFSVDANPVDAVRLSVGGARSQTDLAHALQRIATLLREDRRRGVRAIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="114" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LE06</accession>
    <name>Q1LE06_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 7 8">3,4-dihydroxy-2-butanone 4-phosphate synthase</fullName>
            <shortName evidence="7 8">DHBP synthase</shortName>
            <ecNumber evidence="3 7 8">4.1.99.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7 10" type="primary">ribB</name>
        <name evidence="10" type="ordered locus">Rmet_4758</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="11">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="11">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 7 8">Catalyzes the conversion of D-ribulose 5-phosphate to formate and 3,4-dihydroxy-2-butanone 4-phosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7 8">
            <text>D-ribulose 5-phosphate = (2S)-2-hydroxy-3-oxobutyl phosphate + formate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:18457"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15740"/>
            <dbReference type="ChEBI" id="CHEBI:58121"/>
            <dbReference type="ChEBI" id="CHEBI:58830"/>
            <dbReference type="EC" id="4.1.99.12"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="7 8">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <cofactor evidence="7 8">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="7 8">Binds 2 divalent metal cations per subunit. Magnesium or manganese.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 7 8">Cofactor biosynthesis; riboflavin biosynthesis; 2-hydroxy-3-oxobutyl phosphate from D-ribulose 5-phosphate: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7 8">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7 8">Belongs to the DHBP synthase family.</text>
    </comment>
    <dbReference type="EC" id="4.1.99.12" evidence="3 7 8"/>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11620.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519192.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4758"/>
    <dbReference type="EnsemblBacteria" id="ABF11620">
        <property type="protein sequence ID" value="ABF11620"/>
        <property type="gene ID" value="Rmet_4758"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4758"/>
    <dbReference type="eggNOG" id="COG0108">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020273_3_0_4"/>
    <dbReference type="OMA" id="DAGGLIC"/>
    <dbReference type="OrthoDB" id="900513at2"/>
    <dbReference type="UniPathway" id="UPA00275">
        <property type="reaction ID" value="UER00399"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008686">
        <property type="term" value="F:3,4-dihydroxy-2-butanone-4-phosphate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030145">
        <property type="term" value="F:manganese ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009231">
        <property type="term" value="P:riboflavin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00180">
        <property type="entry name" value="RibB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017945">
        <property type="entry name" value="DHBP_synth_RibB-like_a/b_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000422">
        <property type="entry name" value="DHBP_synthase_RibB"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00926">
        <property type="entry name" value="DHBP_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55821">
        <property type="entry name" value="SSF55821"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00506">
        <property type="entry name" value="ribB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8" id="KW-0456">Lyase</keyword>
    <keyword evidence="7 8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7 8" id="KW-0464">Manganese</keyword>
    <keyword evidence="6 7 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0614">Plasmid</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7 8" id="KW-0686">Riboflavin biosynthesis</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="D-ribulose 5-phosphate binding" evidence="7">
        <location>
            <begin position="52"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="region of interest" description="D-ribulose 5-phosphate binding" evidence="7">
        <location>
            <begin position="165"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="53"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="57"/>
        </location>
        <ligand>
            <name>D-ribulose 5-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58121"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="168"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="site" description="Essential for catalytic activity" evidence="7">
        <location>
            <position position="151"/>
        </location>
    </feature>
    <feature type="site" description="Essential for catalytic activity" evidence="7">
        <location>
            <position position="189"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004904"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012153"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00018836"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022619"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ABF11620.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="228" mass="24299" checksum="18B6FC4E7BBFF666" modified="2006-05-30" version="1">MLTLTKSTSLPDSTAALAPSDADARPLAERIAQALDAMRDGRPVVLLDDDDRENEADLILAAERLTNSNMAMMIRECSGIVCLCLTPEKVRQLGLRPMVENNRSQYGTAFTVSIEAREGVTTGVSAADRITTIRAAIADEAGLDAVVSPGHVFPLVAVEGGVLARRGHTEGSVELARMAGLSPAAVLCELMNPDGTMARRPEALAFAEMYQLPVLTIADLVAWREINA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LE76</accession>
    <name>Q1LE76_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_4688</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11550.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519122.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4688"/>
    <dbReference type="EnsemblBacteria" id="ABF11550">
        <property type="protein sequence ID" value="ABF11550"/>
        <property type="gene ID" value="Rmet_4688"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4688"/>
    <dbReference type="eggNOG" id="ENOG502Z7KR">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_269979_0_0_4"/>
    <dbReference type="OMA" id="TELWHTR"/>
    <dbReference type="OrthoDB" id="849378at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="1185"/>
            <end position="1204"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="1216"/>
            <end position="1233"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="1245"/>
            <end position="1270"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="1282"/>
            <end position="1305"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF11550.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="1385" mass="146790" checksum="056A3CFFDAD28CF0" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LEB3</accession>
    <name>Q1LEB3_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Multidrug resistance protein (HlyD family), EmrA-like secretion</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Rmet_4651</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="5">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="5">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11513.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519088.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4651"/>
    <dbReference type="EnsemblBacteria" id="ABF11513">
        <property type="protein sequence ID" value="ABF11513"/>
        <property type="gene ID" value="Rmet_4651"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4651"/>
    <dbReference type="eggNOG" id="COG1566">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018816_15_1_4"/>
    <dbReference type="OMA" id="TVHTDHA"/>
    <dbReference type="OrthoDB" id="1680841at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039562">
        <property type="entry name" value="MFP_biotin_lipoyl_2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13533">
        <property type="entry name" value="Biotin_lipoyl_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0614">Plasmid</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="31"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="domain" description="Biotin_lipoyl_2" evidence="3">
        <location>
            <begin position="70"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF13533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF11513.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="380" mass="40144" checksum="6E88BB689CC09802" modified="2006-05-30" version="1">MTSTTTASRAPAEAAPSTAAPARRGTNRRALLIRGAGALLVVTAAGYGYHWWTDSRFTEETDDAYVGGDVTLIAAKVPGYVAEVLVTDNQQVHAGDLLARIDDRDYRAALAKAEGAVASEQALIANLDAREKLQQAVIAEARAGVAAVDAETKRARDDQARYASLVEKSAVSIQSSQKADADYKQSVANGQKAKASAEAALRQLDVIATQRQQAQASLTQAIAERDIARLNLGYTELRAPVDGTVGNRRARVGVYAPNGGQLLSIVPARGLWVDANFKEGQLAHMRPGMRATIVADVMPGKVFHGRVESLAPATGAQFSVLPPENATGNFTKIVQRVPVRIVLDDEDARLGTLRPGLSVVAEVDTKAAKVVTADTAASKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LEE0</accession>
    <name>Q1LEE0_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Phospholipase C</fullName>
            <ecNumber evidence="2">3.1.4.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">plcN2</name>
        <name evidence="5" type="ordered locus">Rmet_4624</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="6">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="6">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the bacterial phospholipase C family.</text>
    </comment>
    <dbReference type="EC" id="3.1.4.3" evidence="2"/>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11486.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4624"/>
    <dbReference type="EnsemblBacteria" id="ABF11486">
        <property type="protein sequence ID" value="ABF11486"/>
        <property type="gene ID" value="Rmet_4624"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4624"/>
    <dbReference type="eggNOG" id="COG3511">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008770_1_0_4"/>
    <dbReference type="OMA" id="GYYCSLH"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034480">
        <property type="term" value="F:phosphatidylcholine phospholipase C activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.720.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017850">
        <property type="entry name" value="Alkaline_phosphatase_core_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017767">
        <property type="entry name" value="Bact_PC-PLC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007312">
        <property type="entry name" value="Phosphoesterase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008475">
        <property type="entry name" value="PLipase_C_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006311">
        <property type="entry name" value="TAT_signal"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31956">
        <property type="entry name" value="PTHR31956"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05506">
        <property type="entry name" value="DUF756"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04185">
        <property type="entry name" value="Phosphoesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03396">
        <property type="entry name" value="PC_PLC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51318">
        <property type="entry name" value="TAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0614">Plasmid</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193411" description="Phospholipase C" evidence="3">
        <location>
            <begin position="24"/>
            <end position="745"/>
        </location>
    </feature>
    <feature type="domain" description="DUF756" evidence="4">
        <location>
            <begin position="548"/>
            <end position="636"/>
        </location>
    </feature>
    <feature type="domain" description="DUF756" evidence="4">
        <location>
            <begin position="647"/>
            <end position="729"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009717"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF05506"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABF11486.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="745" mass="81621" checksum="381750AAF0A13A74" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="117" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LEI2</accession>
    <name>Q1LEI2_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Multifunctional: 3-hydroxybutyryl-CoA epimerase, delta(3)-cis-delta(2)-trans-enoyl-CoA isomerase, enoyl-CoA hydratase (N-terminal) 3-hydroxyacyl-CoA dehydrogenase (C-terminal)</fullName>
            <ecNumber evidence="12">1.1.1.35</ecNumber>
            <ecNumber evidence="12">4.2.1.17</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">fadB</name>
        <name evidence="12" type="ordered locus">Rmet_4582</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="13">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="13">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>a (3S)-3-hydroxyacyl-CoA + NAD(+) = a 3-oxoacyl-CoA + H(+) + NADH</text>
            <dbReference type="Rhea" id="RHEA:22432"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57318"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:90726"/>
            <dbReference type="EC" id="1.1.1.35"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Lipid metabolism; fatty acid beta-oxidation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the enoyl-CoA hydratase/isomerase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">In the N-terminal section; belongs to the enoyl-CoA hydratase/isomerase family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.35" evidence="12"/>
    <dbReference type="EC" id="4.2.1.17" evidence="12"/>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11444.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519029.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4582"/>
    <dbReference type="EnsemblBacteria" id="ABF11444">
        <property type="protein sequence ID" value="ABF11444"/>
        <property type="gene ID" value="Rmet_4582"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4582"/>
    <dbReference type="eggNOG" id="COG1024">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG1250">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009834_16_3_4"/>
    <dbReference type="OMA" id="DPLFWKP"/>
    <dbReference type="OrthoDB" id="977512at2"/>
    <dbReference type="UniPathway" id="UPA00659"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003857">
        <property type="term" value="F:3-hydroxyacyl-CoA dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004300">
        <property type="term" value="F:enoyl-CoA hydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016853">
        <property type="term" value="F:isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070403">
        <property type="term" value="F:NAD+ binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006635">
        <property type="term" value="P:fatty acid beta-oxidation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006176">
        <property type="entry name" value="3-OHacyl-CoA_DH_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006108">
        <property type="entry name" value="3HC_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008927">
        <property type="entry name" value="6-PGluconate_DH-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029045">
        <property type="entry name" value="ClpP/crotonase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018376">
        <property type="entry name" value="Enoyl-CoA_hyd/isom_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001753">
        <property type="entry name" value="Enoyl-CoA_hydra/iso"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00725">
        <property type="entry name" value="3HCDH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02737">
        <property type="entry name" value="3HCDH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00378">
        <property type="entry name" value="ECH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48179">
        <property type="entry name" value="SSF48179"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52096">
        <property type="entry name" value="SSF52096"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00166">
        <property type="entry name" value="ENOYL_COA_HYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="12" id="KW-0413">Isomerase</keyword>
    <keyword evidence="5" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="7" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="12" id="KW-0456">Lyase</keyword>
    <keyword evidence="6" id="KW-0520">NAD</keyword>
    <keyword evidence="12" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="12" id="KW-0614">Plasmid</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="3HCDH_N" evidence="11">
        <location>
            <begin position="297"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="domain" description="3HCDH" evidence="10">
        <location>
            <begin position="478"/>
            <end position="570"/>
        </location>
    </feature>
    <feature type="domain" description="3HCDH" evidence="10">
        <location>
            <begin position="606"/>
            <end position="693"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023693"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF02737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ABF11444.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="696" mass="73494" checksum="3FD499AD5C456B39" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="116" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LEK7</accession>
    <name>Q1LEK7_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Peptide methionine sulfoxide reductase MsrA</fullName>
            <shortName evidence="4">Protein-methionine-S-oxide reductase</shortName>
            <ecNumber evidence="4">1.8.4.11</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">Peptide-methionine (S)-S-oxide reductase</fullName>
            <shortName evidence="4">Peptide Met(O) reductase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="4 6" type="primary">msrA</name>
        <name evidence="6" type="ordered locus">Rmet_4557</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="7">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="7">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Has an important function as a repair enzyme for proteins that have been inactivated by oxidation. Catalyzes the reversible oxidation-reduction of methionine sulfoxide in proteins to methionine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 4">
            <text>[thioredoxin]-disulfide + H2O + L-methionine = [thioredoxin]-dithiol + L-methionine (S)-S-oxide</text>
            <dbReference type="Rhea" id="RHEA:19993"/>
            <dbReference type="Rhea" id="RHEA-COMP:10698"/>
            <dbReference type="Rhea" id="RHEA-COMP:10700"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:50058"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:58772"/>
            <dbReference type="EC" id="1.8.4.11"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2 4">
            <text>[thioredoxin]-disulfide + H2O + L-methionyl-[protein] = [thioredoxin]-dithiol + L-methionyl-(S)-S-oxide-[protein]</text>
            <dbReference type="Rhea" id="RHEA:14217"/>
            <dbReference type="Rhea" id="RHEA-COMP:10698"/>
            <dbReference type="Rhea" id="RHEA-COMP:10700"/>
            <dbReference type="Rhea" id="RHEA-COMP:12313"/>
            <dbReference type="Rhea" id="RHEA-COMP:12315"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16044"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:44120"/>
            <dbReference type="ChEBI" id="CHEBI:50058"/>
            <dbReference type="EC" id="1.8.4.11"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the MsrA Met sulfoxide reductase family.</text>
    </comment>
    <dbReference type="EC" id="1.8.4.11" evidence="4"/>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11419.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519009.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4557"/>
    <dbReference type="EnsemblBacteria" id="ABF11419">
        <property type="protein sequence ID" value="ABF11419"/>
        <property type="gene ID" value="Rmet_4557"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4557"/>
    <dbReference type="eggNOG" id="COG0225">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031040_10_2_4"/>
    <dbReference type="OMA" id="WVLPRRS"/>
    <dbReference type="OrthoDB" id="1554384at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033744">
        <property type="term" value="F:L-methionine:thioredoxin-disulfide S-oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008113">
        <property type="term" value="F:peptide-methionine (S)-S-oxide reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1060.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01401">
        <property type="entry name" value="MsrA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002569">
        <property type="entry name" value="Met_Sox_Rdtase_MsrA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036509">
        <property type="entry name" value="Met_Sox_Rdtase_MsrA_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01625">
        <property type="entry name" value="PMSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55068">
        <property type="entry name" value="SSF55068"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00401">
        <property type="entry name" value="msrA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4 6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-0614">Plasmid</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PMSR" evidence="5">
        <location>
            <begin position="7"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="active site" evidence="4">
        <location>
            <position position="14"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001497"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01401"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01625"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABF11419.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="168" mass="18955" checksum="BA4C59128860D15C" modified="2006-05-30" version="1">MTTQIETAILAGGCFWGMQDLLRRYPGVLSTRVGYTGGDVPNATYRHHGTHAEGIEIAFDPSQISYRQILEFFFQIHDPTTKNRQGNDVGTSYRSAIFYLNDEQKQTAEDTVADVNASGLWPGKVVTEIAPAGPFWDAEPEHQDYLERYPNGYTCHFIRPGWKLPKRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LEV9</accession>
    <name>Q1LEV9_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Response regulator receiver domain protein (CheY-like)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_4452</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11317.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518921.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4452"/>
    <dbReference type="EnsemblBacteria" id="ABF11317">
        <property type="protein sequence ID" value="ABF11317"/>
        <property type="gene ID" value="Rmet_4452"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4452"/>
    <dbReference type="eggNOG" id="COG2197">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_69_15_4"/>
    <dbReference type="OMA" id="MISRTNT"/>
    <dbReference type="OrthoDB" id="2015384at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Response regulatory" evidence="2">
        <location>
            <begin position="11"/>
            <end position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF11317.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="136" mass="15009" checksum="41F6BF5DF7C4564F" modified="2006-05-30" version="1">MISRTNTDRLTVYLVERTPSVRRRQLRLLASLDGLRVAGAGHDAQAHQTELVDLQPDVVLIGLNATENTPLPRIRALAIALPASTLIVLANEGTPQMRRACLMAGVAHCFDKTLEIVELRNLLLAMASTARQHRQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="100" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LEY0</accession>
    <name>Q1LEY0_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">6-phosphogluconate dehydrogenase, NAD-binding</fullName>
            <ecNumber evidence="5">1.1.1.31</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Rmet_4431</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="6">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="6">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EC" id="1.1.1.31" evidence="5"/>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11296.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4431"/>
    <dbReference type="EnsemblBacteria" id="ABF11296">
        <property type="protein sequence ID" value="ABF11296"/>
        <property type="gene ID" value="Rmet_4431"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4431"/>
    <dbReference type="eggNOG" id="COG2084">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035117_1_1_4"/>
    <dbReference type="OMA" id="NYIMASS"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008442">
        <property type="term" value="F:3-hydroxyisobutyrate dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016054">
        <property type="term" value="P:organic acid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1040.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008927">
        <property type="entry name" value="6-PGluconate_DH-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013328">
        <property type="entry name" value="6PGD_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006115">
        <property type="entry name" value="6PGDH_NADP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015815">
        <property type="entry name" value="HIBADH-related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029154">
        <property type="entry name" value="NADP-bd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14833">
        <property type="entry name" value="NAD_binding_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03446">
        <property type="entry name" value="NAD_binding_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000103">
        <property type="entry name" value="HIBADH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48179">
        <property type="entry name" value="SSF48179"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0520">NAD</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-0614">Plasmid</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAD_binding_2" evidence="3">
        <location>
            <begin position="6"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="domain" description="NAD_binding_11" evidence="4">
        <location>
            <begin position="168"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="active site" evidence="2">
        <location>
            <position position="174"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR000103-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF03446"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF14833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABF11296.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="293" mass="30187" checksum="CBF6F2DA252E30D2" modified="2006-05-30" version="1">MQAIKTIGFIGVGVMGEPMCRHLAQKSGATVLACDRDAAPLARLAECGVRAATMAEVSAQADIVFLSLPSGEVVESVVLGENGLLAGGRAGQIIVDLSTSTMNTARKAAEAMAARGMKFADAPVMRTRAAAEAGTLAVPVGADDALFALVAPFIRMFASDVVHAGGVGAGQVVKILNNMVLYETVLALSEARAIGVRAGLSPQGLFEALSIGSADSFALRNHGMKAIAPQVFPERAFPVTYARKDISYAVALADQLGVEATGAKNLMNCLDQAIEAGHGDKYAPAISLVFEGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="62" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LF43</accession>
    <name>Q1LF43_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_4368</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11233.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518850.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4368"/>
    <dbReference type="EnsemblBacteria" id="ABF11233">
        <property type="protein sequence ID" value="ABF11233"/>
        <property type="gene ID" value="Rmet_4368"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4368"/>
    <dbReference type="HOGENOM" id="CLU_2715607_0_0_4"/>
    <dbReference type="OrthoDB" id="2062286at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="47"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="53"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF11233.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="72" mass="7315" checksum="CB46C7E410F9E36D" modified="2006-05-30" version="1">MNAVLLSAIALGLIATGVAVTYLLATRLPMRLLATAQDHGRFAGLGSTQEIDGAGRATPSTSTSVQPQLAHA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="69" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LF81</accession>
    <name>Q1LF81_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative ABC transporter periplasmic substrate-binding protein, Twin-arginine translocation pathway signal</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_4330</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11195.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518819.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4330"/>
    <dbReference type="EnsemblBacteria" id="ABF11195">
        <property type="protein sequence ID" value="ABF11195"/>
        <property type="gene ID" value="Rmet_4330"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4330"/>
    <dbReference type="eggNOG" id="COG0715">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028871_9_0_4"/>
    <dbReference type="OMA" id="LILMANR"/>
    <dbReference type="OrthoDB" id="1303199at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015168">
        <property type="entry name" value="SsuA/THI5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09084">
        <property type="entry name" value="NMT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NMT1" evidence="1">
        <location>
            <begin position="59"/>
            <end position="261"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF09084"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF11195.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="348" mass="37531" checksum="E997929F3B009E7D" modified="2006-05-30" version="1">MSSVFEASYVNGGRRSLLRGIGAAAAGVSGVLPGLSLAQQREQATLATGSAGYTWALTAVAESAGYWKKRNVDLRVQDFTTGRDAMQALLGGSANFSTTTDTPLVFAVLQGLKPAVLANFSRYSYDMKIVASERSGIDPRNPASLRGKKIGTPAGTSGQYALAKYLEFAKLAPGDITQINIAPSDLVGALVRGDIDAFSWTALAGSAAQRQSGGKVVFLTQNGYEKYFRSHQLLLVNQATLSSRKPLLSDAVNALLDAENHIHTNAAWPEQVSSRVRSTPEEIRQFTSTFEFKVEFNKSFVEDLVSQAQWAIDNKLAPRPTGDLRELFRSVIHDETLKSINPSRVSLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="61" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LFC6</accession>
    <name>Q1LFC6_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Bacteriolytic lipoprotein entericidin AB</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">encAB</name>
        <name evidence="6" type="ordered locus">Rmet_4284</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="7">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="7">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the EcnA/EcnB lipoprotein family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11150.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008652347.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF11150">
        <property type="protein sequence ID" value="ABF11150"/>
        <property type="gene ID" value="Rmet_4284"/>
    </dbReference>
    <dbReference type="GeneID" id="60823144"/>
    <dbReference type="KEGG" id="rme:Rmet_4284"/>
    <dbReference type="eggNOG" id="COG5510">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_193827_3_0_4"/>
    <dbReference type="OrthoDB" id="2055071at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009636">
        <property type="term" value="P:response to toxic substance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012556">
        <property type="entry name" value="Entericidin"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08085">
        <property type="entry name" value="Entericidin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0614">Plasmid</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="26"/>
            <end position="47"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABF11150.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="47" mass="4925" checksum="4E1623D221ED4503" modified="2006-05-30" version="1">MRKLYALITLAGMLLIAGCNTISGAGKDIERGGEKVQGAAESTKQKM</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-12-02" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LFF7</accession>
    <name>Q1LFF7_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Fimbrial protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_4253</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11119.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4253"/>
    <dbReference type="EnsemblBacteria" id="ABF11119">
        <property type="protein sequence ID" value="ABF11119"/>
        <property type="gene ID" value="Rmet_4253"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4253"/>
    <dbReference type="eggNOG" id="COG3539">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_058392_1_0_4"/>
    <dbReference type="OMA" id="YPITIHA"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009289">
        <property type="term" value="C:pilus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007155">
        <property type="term" value="P:cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1090">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000259">
        <property type="entry name" value="Adhesion_dom_fimbrial"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036937">
        <property type="entry name" value="Adhesion_dom_fimbrial_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008966">
        <property type="entry name" value="Adhesion_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00419">
        <property type="entry name" value="Fimbrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49401">
        <property type="entry name" value="SSF49401"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193447" evidence="1">
        <location>
            <begin position="32"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="domain" description="Fimbrial" evidence="2">
        <location>
            <begin position="190"/>
            <end position="336"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00419"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF11119.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="336" mass="34612" checksum="5179E8359FB6C61F" modified="2006-05-30" version="1">MNVTYRTIKAALRWFVLPVLLALGVNHGALAACTVTTANPDYIYTLPSTLTVARNAPVGTVILSYAPPGQNNPTASGTCPVTAYAVDAMAGSWTTTSVADVYATNVPGIGIKVLGKASNHPVPLLPTVYQWSGSATGPFSGYWDSFGYQLVVTGPVSPGTLSFASPVASMWLSTSTTALVDAAVVTNLQINGTTNIVARACTTPDVTVNLGNHLSSEFSGVGSKTSSTSFNIALNGCPSGLNTIQYQIDPVTSIVSGTGNTVVALNSGSTANRVGVQLLDGNGNPLALSSPITFSGYSSSTGGDYTIPLKARYYQIDTPVGPGSANTSMIFTMTYQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LFR0</accession>
    <name>Q1LFR0_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Phytoene synthase</fullName>
            <ecNumber evidence="2">2.5.1.32</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">crtB</name>
        <name evidence="2" type="ordered locus">Rmet_4149</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EC" id="2.5.1.32" evidence="2"/>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11016.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518641.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4149"/>
    <dbReference type="EnsemblBacteria" id="ABF11016">
        <property type="protein sequence ID" value="ABF11016"/>
        <property type="gene ID" value="Rmet_4149"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4149"/>
    <dbReference type="eggNOG" id="COG1562">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037269_1_1_4"/>
    <dbReference type="OMA" id="VKAPRIM"/>
    <dbReference type="OrthoDB" id="1449625at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004311">
        <property type="term" value="F:farnesyltranstransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016767">
        <property type="term" value="F:geranylgeranyl-diphosphate geranylgeranyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016117">
        <property type="term" value="P:carotenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00683">
        <property type="entry name" value="Trans_IPPS_HH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.600.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008949">
        <property type="entry name" value="Isoprenoid_synthase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017828">
        <property type="entry name" value="SQ_synth_HpnD-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002060">
        <property type="entry name" value="Squ/phyt_synthse"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019845">
        <property type="entry name" value="Squalene/phytoene_synthase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044843">
        <property type="entry name" value="Trans_IPPS_bact-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033904">
        <property type="entry name" value="Trans_IPPS_HH"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00494">
        <property type="entry name" value="SQS_PSY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01212">
        <property type="entry name" value="Phytoene_synthase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48576">
        <property type="entry name" value="SSF48576"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03465">
        <property type="entry name" value="HpnD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01045">
        <property type="entry name" value="SQUALEN_PHYTOEN_SYN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF11016.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="282" mass="30221" checksum="7D6B3B33041E07AB" modified="2006-05-30" version="1">MAGTEIATPGGPIAGPAVSSGSSFHAAMRILPASQRQAMFEIYAFCRAVDDIADGDTQHADRLAALEQWRADIAACYAGQAPAALRPLDEQIKAFNLQQQDFSDVIDGMIMDVVATIVAPDAATLDTYCDRVASAVGRLAVRVFGLDEAPGIALAHHLGRALQLTNILRDLDEDAEIGRLYLPAEALAAAGIPVGKPYDVIHHPAIAEVCTGIARDAQAHYDASAAIMARCPARVVRSPRIMSDVYHGILTRLLARGWQPPRARVRVPKAKLVWILLRHAIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LFS3</accession>
    <name>Q1LFS3_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Taurine dioxygenase (TauD/TfdA family)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">tauD</name>
        <name evidence="3" type="ordered locus">Rmet_4136</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11003.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518628.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4136"/>
    <dbReference type="EnsemblBacteria" id="ABF11003">
        <property type="protein sequence ID" value="ABF11003"/>
        <property type="gene ID" value="Rmet_4136"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4136"/>
    <dbReference type="eggNOG" id="COG2175">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036005_2_1_4"/>
    <dbReference type="OMA" id="DAGHYWH"/>
    <dbReference type="OrthoDB" id="1742732at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051213">
        <property type="term" value="F:dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.130.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042098">
        <property type="entry name" value="TauD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003819">
        <property type="entry name" value="TauD/TfdA-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02668">
        <property type="entry name" value="TauD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TauD" evidence="2">
        <location>
            <begin position="29"/>
            <end position="301"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF11003.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="308" mass="34624" checksum="5544B93A7BF2F233" modified="2006-05-30" version="1">MTIATADASVDTSANLATESSTRSLQDFEIRPLGAALGAEVIGVDLGRPLSHGDFQRIHQAHLDHHVLVFRDQRITPAQQIAFSRRFGPLQIHVLNQFQLPGHPEVLIVSNVIENGKPIGLGDAGHFWHSDLSYKETPSLGSLLHARELPAEGGDTLFANMHTAWESLPEALQRKVADLTAEHTYLARYAELQARSPWRPNLTPEQIAQVKPVVHPVVRTHPETGRRALFVSEHFTTRIVGLPDAESRAILDELFAHSVKPEHVYRHQWREHDLVFWDNRSLLHLAAGTPDQLRRVMYRTTIEGDQPF</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-10-07" version="51" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LFU1</accession>
    <name>Q1LFU1_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_4118</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10985.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4118"/>
    <dbReference type="EnsemblBacteria" id="ABF10985">
        <property type="protein sequence ID" value="ABF10985"/>
        <property type="gene ID" value="Rmet_4118"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4118"/>
    <dbReference type="HOGENOM" id="CLU_2540129_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF10985.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="83" mass="9147" checksum="7BEC2E95381DC121" modified="2006-05-30" version="1">MILRHSIVTDRNNGPGGGRQVPLAAPTADRRGAPARRAPGEPPPRMRREAHFNPVSPIPLHHRFLPYANRWIGIPAKCPGMSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LFW4</accession>
    <name>Q1LFW4_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">SnoaL-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_4095</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10962.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518589.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4095"/>
    <dbReference type="EnsemblBacteria" id="ABF10962">
        <property type="protein sequence ID" value="ABF10962"/>
        <property type="gene ID" value="Rmet_4095"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4095"/>
    <dbReference type="eggNOG" id="ENOG50325UW">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_125060_2_1_4"/>
    <dbReference type="OMA" id="FRYQPIA"/>
    <dbReference type="OrthoDB" id="1907687at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032710">
        <property type="entry name" value="NTF2-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037401">
        <property type="entry name" value="SnoaL-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12680">
        <property type="entry name" value="SnoaL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54427">
        <property type="entry name" value="SSF54427"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SnoaL-like" evidence="1">
        <location>
            <begin position="24"/>
            <end position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12680"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF10962.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="121" mass="13602" checksum="3F9383EFD3725321" modified="2006-05-30" version="1">MQNSIPTLLRRNLEEVFGEIDPARRRRAIDDIFTDDSVFYDPNSGIHRGRDAIDRIAGVIKASHPDFRYQPIAEPEVAGDGGRIRWVSGRPGEAPAYAGTDFIVTRDGRIVAVYLFFDELP</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="98" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LFW7</accession>
    <name>Q1LFW7_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Transcriptional regulator, LysR family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_4092</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the LysR transcriptional regulatory family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10959.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518586.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4092"/>
    <dbReference type="EnsemblBacteria" id="ABF10959">
        <property type="protein sequence ID" value="ABF10959"/>
        <property type="gene ID" value="Rmet_4092"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4092"/>
    <dbReference type="eggNOG" id="COG0583">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039613_16_2_4"/>
    <dbReference type="OMA" id="RFMVRYP"/>
    <dbReference type="OrthoDB" id="750230at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005119">
        <property type="entry name" value="LysR_subst-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000847">
        <property type="entry name" value="Tscrpt_reg_HTH_LysR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00126">
        <property type="entry name" value="HTH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03466">
        <property type="entry name" value="LysR_substrate"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50931">
        <property type="entry name" value="HTH_LYSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH lysR-type" evidence="2">
        <location>
            <begin position="5"/>
            <end position="62"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF10959.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="305" mass="33439" checksum="CAB482A067D009D8" modified="2006-05-30" version="1">MDQIPDLNDLRLFAEVVDRGSFTAAARSLGQQTSKLSRRIRALEEALGVRLLNRTSRSLSLTETGRRFHQHCLAVVAEARAAKEIVDQTRSQPQGTVRISCPVALLSSGVAETISRYVHDNPQVQVLVDATNRRVDVIEEGLDFAIRVRPAPLEDTDLAVRQLGLSTFVLVASPELARLHAPPASIESLQDWPTIAMASNNERYVWNLVDAGGKEQAFAHRPRLATDDIASLRIAAMFGVGVAMLPREFIEEDLQSGRLLHLLPELASTSGLVHAIFPTRRGMVPAVRHLLDALVAGFEAVNQGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="100" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LG62</accession>
    <name>Q1LG62_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Glucans biosynthesis protein G</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">mdoG</name>
        <name evidence="6" type="synonym">opgG</name>
        <name evidence="8" type="ordered locus">Rmet_3996</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="9">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="9">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Involved in the biosynthesis of osmoregulated periplasmic glucans (OPGs).</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 6">Glycan metabolism; osmoregulated periplasmic glucan (OPG) biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Periplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 6">Belongs to the OpgD/OpgG family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10864.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3996"/>
    <dbReference type="EnsemblBacteria" id="ABF10864">
        <property type="protein sequence ID" value="ABF10864"/>
        <property type="gene ID" value="Rmet_3996"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3996"/>
    <dbReference type="eggNOG" id="COG3131">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023403_2_0_4"/>
    <dbReference type="OMA" id="QENAAIW"/>
    <dbReference type="UniPathway" id="UPA00637"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042597">
        <property type="term" value="C:periplasmic space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009250">
        <property type="term" value="P:glucan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.98.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01069">
        <property type="entry name" value="MdoG_OpgG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011013">
        <property type="entry name" value="Gal_mutarotase_sf_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014718">
        <property type="entry name" value="GH-type_carb-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014438">
        <property type="entry name" value="Glucan_biosyn_MdoG/MdoD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007444">
        <property type="entry name" value="Glucan_biosyn_MdoG_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023704">
        <property type="entry name" value="MdoG_OpgG"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30504">
        <property type="entry name" value="PTHR30504"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04349">
        <property type="entry name" value="MdoG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006281">
        <property type="entry name" value="MdoG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF74650">
        <property type="entry name" value="SSF74650"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="SSF81296"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0574">Periplasm</keyword>
    <keyword evidence="8" id="KW-0614">Plasmid</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5009018926" description="Glucans biosynthesis protein G" evidence="6">
        <location>
            <begin position="37"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="domain" description="MdoG" evidence="7">
        <location>
            <begin position="37"/>
            <end position="520"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022764"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF04349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ABF10864.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="536" mass="59855" checksum="FAA9BFE1CCBE5CD5" modified="2006-05-30" version="1" precursor="true"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="92" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LGD4</accession>
    <name>Q1LGD4_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Efflux pump membrane transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">acrB</name>
        <name evidence="5" type="ordered locus">Rmet_3924</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="6">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="6">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 4">Cell inner membrane</location>
            <topology evidence="2 4">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 4">Belongs to the resistance-nodulation-cell division (RND) (TC 2.A.6) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10792.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518440.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3924"/>
    <dbReference type="EnsemblBacteria" id="ABF10792">
        <property type="protein sequence ID" value="ABF10792"/>
        <property type="gene ID" value="Rmet_3924"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3924"/>
    <dbReference type="eggNOG" id="COG0841">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002755_0_1_4"/>
    <dbReference type="OMA" id="QYESFII"/>
    <dbReference type="OrthoDB" id="91817at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015562">
        <property type="term" value="F:efflux transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042908">
        <property type="term" value="P:xenobiotic transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2090.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027463">
        <property type="entry name" value="AcrB_DN_DC_subdom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001036">
        <property type="entry name" value="Acrflvin-R"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004764">
        <property type="entry name" value="HAE1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32063">
        <property type="entry name" value="PTHR32063"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00873">
        <property type="entry name" value="ACR_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00702">
        <property type="entry name" value="ACRIFLAVINRP"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82714">
        <property type="entry name" value="SSF82714"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00915">
        <property type="entry name" value="2A0602"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0614">Plasmid</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="340"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="366"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="437"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="470"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="539"/>
            <end position="556"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="874"/>
            <end position="891"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="898"/>
            <end position="918"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="924"/>
            <end position="948"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="969"/>
            <end position="989"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="1001"/>
            <end position="1027"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004429"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU364070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABF10792.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="1048" mass="113214" checksum="95518BBD5C49AC2C" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="58" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LGE8</accession>
    <name>Q1LGE8_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_3910</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10778.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3910"/>
    <dbReference type="EnsemblBacteria" id="ABF10778">
        <property type="protein sequence ID" value="ABF10778"/>
        <property type="gene ID" value="Rmet_3910"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3910"/>
    <dbReference type="eggNOG" id="ENOG502ZNEU">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2094830_0_0_4"/>
    <dbReference type="OMA" id="VWSEAAH"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF10778.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="116" mass="12715" checksum="9D2A4263C7D1C898" modified="2006-05-30" version="1">MVCRKESADALQTQRPDVKQEIAMFQNTFAQADAVIVHDAAIAAAIDAAMLRRRDEYAGQPMAWKVFCEASHVATLCEPLRMAFINRVASERGADIALRLKTKAEAIRAAAIAELV</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LGI3</accession>
    <name>Q1LGI3_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_3875</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the LysR transcriptional regulatory family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10743.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518390.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3875"/>
    <dbReference type="EnsemblBacteria" id="ABF10743">
        <property type="protein sequence ID" value="ABF10743"/>
        <property type="gene ID" value="Rmet_3875"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3875"/>
    <dbReference type="eggNOG" id="COG0583">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039613_16_1_4"/>
    <dbReference type="OMA" id="IVPDWHF"/>
    <dbReference type="OrthoDB" id="1661500at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005119">
        <property type="entry name" value="LysR_subst-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000847">
        <property type="entry name" value="Tscrpt_reg_HTH_LysR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00126">
        <property type="entry name" value="HTH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03466">
        <property type="entry name" value="LysR_substrate"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00039">
        <property type="entry name" value="HTHLYSR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50931">
        <property type="entry name" value="HTH_LYSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH lysR-type" evidence="2">
        <location>
            <begin position="1"/>
            <end position="59"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF10743.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="307" mass="33650" checksum="4D25824E7E543F60" modified="2006-05-30" version="1">MEALNLLESFIQSAETGSFSAAARRLGLTPAAISKNVARLENHLGLRLFQRSTRRLTLTMGGEQFLQQVSDPFSTLRDAFASAAQEDGKPAGVLRVSMGVAFGREYLVPLLGEFLARYPGIVPDWHFENRTVDLIAGGFDAAIGGGIELTEGVVARELARTYVVVAASPAYMKDRAMPRHPSDLAGFDGIVRRSASTGRLRHWNLRNQIGEQALAEMRTRVILDDPEAMAHAAMSGLGIALLPMPHAAPWLESGALVRLLPGWYADNGPIVLYYPNRKLLPPKTRVFIDFIAESFQKNALAHRFDGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LGM8</accession>
    <name>Q1LGM8_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Metal-dependent phosphohydrolase, HD subdomain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_3828</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10698.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3828"/>
    <dbReference type="EnsemblBacteria" id="ABF10698">
        <property type="protein sequence ID" value="ABF10698"/>
        <property type="gene ID" value="Rmet_3828"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3828"/>
    <dbReference type="eggNOG" id="COG1896">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039453_5_1_4"/>
    <dbReference type="OMA" id="AEHSWHF"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002953">
        <property type="term" value="F:5'-deoxynucleotidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006674">
        <property type="entry name" value="HD_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039356">
        <property type="entry name" value="YfbR/HDDC2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11845">
        <property type="entry name" value="PTHR11845"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13023">
        <property type="entry name" value="HD_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HD" evidence="1">
        <location>
            <begin position="45"/>
            <end position="207"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF10698.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="224" mass="24846" checksum="14DCC74F7ED2257D" modified="2006-05-30" version="1">MSDGRRTAPRGRGGLLNFTAKHCPSAFNVNLSNLERQLSFLREIDLLKSVVRMTPLINQSRRENSAEHSWHLAMYALVLSEHAAAPVDALRVVKMLLIHDIVEIDAGDVPFHDPAARAGQAALEEQAAERIFSLLPAAQAAEFRSLWSEFEAGESDDARFAKSLDRLQPLLHNAATGGGSWVECDVTEEQAMARYGPPIERGSPALWEEASRLVREHFQKPREG</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="66" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LGV5</accession>
    <name>Q1LGV5_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Methyl-accepting chemotaxis protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_3749</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10621.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518269.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3749"/>
    <dbReference type="EnsemblBacteria" id="ABF10621">
        <property type="protein sequence ID" value="ABF10621"/>
        <property type="gene ID" value="Rmet_3749"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3749"/>
    <dbReference type="eggNOG" id="ENOG502ZB0I">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_053685_0_0_4"/>
    <dbReference type="OMA" id="IEGINMQ"/>
    <dbReference type="OrthoDB" id="1451426at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="300"/>
            <end position="318"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF10621.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="368" mass="38432" checksum="1C5530169BE64A44" modified="2006-05-30" version="1">MPLPFIIGAAALVTAAYGAKKGYDGYQKHSEADEIVKDAQARYERRKSAVDAQEEKTTLALDLLGKKELEIGKSIGEFKTLADKLLQQLNAGRDNKLNISIPKHELQKIENYSITAISVLGTAVGAGAAGAAVGFAVYGGVMALGAASTGTAISALSGVAATNATLAALGGGALSAGGLGMAGGTVILGAAVAAPVLAIAGWAYDSHGEEALINARKANDEVSRAVEKLMKASEQLAKTEVYARKIKSELTTISGQFQQYFDTLKGINRFIDDIKGRNVDVGAEIEKLGDDVLRVIENGFALAAILVNLIIVPIFRPAKSNGKVVHNKDGVPEMEKDADGSMILNDAELDLALRRAESQAGNIEAVRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="98" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LHB7</accession>
    <name>Q1LHB7_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">DNA-binding transcriptional repressor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">acrR</name>
        <name evidence="4" type="ordered locus">Rmet_3587</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10459.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011518001.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3587"/>
    <dbReference type="EnsemblBacteria" id="ABF10459">
        <property type="protein sequence ID" value="ABF10459"/>
        <property type="gene ID" value="Rmet_3587"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3587"/>
    <dbReference type="eggNOG" id="COG1309">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069356_12_3_4"/>
    <dbReference type="OMA" id="MMEIIFH"/>
    <dbReference type="OrthoDB" id="2047542at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023772">
        <property type="entry name" value="DNA-bd_HTH_TetR-type_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001647">
        <property type="entry name" value="HTH_TetR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036271">
        <property type="entry name" value="Tet_transcr_reg_TetR-rel_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013572">
        <property type="entry name" value="Tscrpt_reg_MAATS_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08361">
        <property type="entry name" value="TetR_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00440">
        <property type="entry name" value="TetR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00455">
        <property type="entry name" value="HTHTETR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48498">
        <property type="entry name" value="SSF48498"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01081">
        <property type="entry name" value="HTH_TETR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50977">
        <property type="entry name" value="HTH_TETR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0678">Repressor</keyword>
    <feature type="domain" description="HTH tetR-type" evidence="3">
        <location>
            <begin position="10"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="H-T-H motif" evidence="2">
        <location>
            <begin position="33"/>
            <end position="52"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF10459.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="242" mass="27206" checksum="B26EF90B7754060F" modified="2006-05-30" version="1">MVRRTKEEALETRNRILDAAEAVFHARGVARPSLADIAEAAGVTRGAIYWHFKNKSDVFAAMCDRVNLPVEALCSPERIASQEDPLGSIRDICAFVFRQTVNNPQWRRVFEIIFHKCEMVEDNGAIFVRQRQSHQEGTVKMREHLRLAMERGQLPADLDLPLAVNAFHAAIGGVLAHWLFSPEDFELAREAERLADAFIDTLRLSPALRVGYVPRPMAPLDAEMAALCERQPQQPQQALAPT</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LHF1</accession>
    <name>Q1LHF1_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">PNPLA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Rmet_3553</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10425.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517969.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3553"/>
    <dbReference type="EnsemblBacteria" id="ABF10425">
        <property type="protein sequence ID" value="ABF10425"/>
        <property type="gene ID" value="Rmet_3553"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3553"/>
    <dbReference type="eggNOG" id="COG1752">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_930250_0_0_4"/>
    <dbReference type="OMA" id="AYWGNLL"/>
    <dbReference type="OrthoDB" id="373926at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016035">
        <property type="entry name" value="Acyl_Trfase/lysoPLipase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002641">
        <property type="entry name" value="PNPLA_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01734">
        <property type="entry name" value="Patatin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52151">
        <property type="entry name" value="SSF52151"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51635">
        <property type="entry name" value="PNPLA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="1 2" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PNPLA" evidence="3">
        <location>
            <begin position="18"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="GXSXG" evidence="2">
        <location>
            <begin position="50"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="DGA/G" evidence="2">
        <location>
            <begin position="210"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="2">
        <location>
            <position position="52"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="2">
        <location>
            <position position="210"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF10425.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="303" mass="34194" checksum="2E5FDA2996CB5D6E" modified="2006-05-30" version="1">MSTSTSLPSTSAQPFDQIVFAGGGNRCWWQAGWWDTVEPELKLRPRVIAGISAGAATACMVYANDSKQTMAYYREVLANNSRNAYWGNLLRRERVFPHYGIYRNALLSLFGDSRLERLQEAPEIRIGVAHIPRWSGPRLAVAAGLLAYNIDKHLLKTLHPRLGRKLGFHPEFVRAQDCRSPEDLADLLLQSASTPPFTPVLRRDGRAVLDGGLVDNVPVDALDATPGNVLVLVTRLYPRPRRFVLEVDGQRRLYLQPSMRVPISSWDYTRPEAMTHAYDLGCRDAETFLREWPEIREELLPAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="95" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LHJ5</accession>
    <name>Q1LHJ5_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Transcriptional regulator, LysR-family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_3509</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the LysR transcriptional regulatory family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10381.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517933.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3509"/>
    <dbReference type="EnsemblBacteria" id="ABF10381">
        <property type="protein sequence ID" value="ABF10381"/>
        <property type="gene ID" value="Rmet_3509"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3509"/>
    <dbReference type="eggNOG" id="COG0583">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039613_37_1_4"/>
    <dbReference type="OMA" id="DEWQDYY"/>
    <dbReference type="OrthoDB" id="846433at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005119">
        <property type="entry name" value="LysR_subst-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000847">
        <property type="entry name" value="Tscrpt_reg_HTH_LysR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00126">
        <property type="entry name" value="HTH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03466">
        <property type="entry name" value="LysR_substrate"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00039">
        <property type="entry name" value="HTHLYSR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50931">
        <property type="entry name" value="HTH_LYSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH lysR-type" evidence="2">
        <location>
            <begin position="6"/>
            <end position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF10381.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="300" mass="33214" checksum="8D64DE64D9E1E683" modified="2006-05-30" version="1">MRIRSPSMSELHAFATAARLGSFTRAAETLCVTQGAISRAISRLESHFGQPLIHRNAHRLTLTDAGRSYLEAIAVHLAAIETASASMLATDRRHHLILSAVPTLASVWLVPRLPDFHRRHPEIHLDFVPYRRDEDFSGARPDAAILAGEAGQWPGWQADYVIGREMVPVCHPARAETRQWQSPDELLDEPLLYHTTAPANWQNWLRAAGVPSAAPTLARGFDQVSILLEAVKADMGVAVLQRCLVRDALQAGQVIAPFDLPIRLNRGYFLCAPRERRDHPALTAFRDWLLSAAAIDIGSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LHT6</accession>
    <name>Q1LHT6_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Thiol-disulfide isomerase-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">dsbX</name>
        <name evidence="3" type="ordered locus">Rmet_3418</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10290.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3418"/>
    <dbReference type="EnsemblBacteria" id="ABF10290">
        <property type="protein sequence ID" value="ABF10290"/>
        <property type="gene ID" value="Rmet_3418"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3418"/>
    <dbReference type="eggNOG" id="COG0526">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_042529_11_2_4"/>
    <dbReference type="OMA" id="WASWCPF"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016853">
        <property type="term" value="F:isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013740">
        <property type="entry name" value="Redoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08534">
        <property type="entry name" value="Redoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="SSF52833"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0413">Isomerase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193562" evidence="1">
        <location>
            <begin position="28"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="domain" description="Thioredoxin" evidence="2">
        <location>
            <begin position="22"/>
            <end position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF10290.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="166" mass="18248" checksum="E9557199AF9E97D0" modified="2006-05-30" version="1">MRARRGVVAWMLALVAAFGAAAQLAQAAEVGDVIHLPDVKLLDGRTVPASQWAGKPLVVEIWASWCPFCALQNPRLQALYDKTRGTPLQVLTISIDKHPHEAVAYLKQRGYTFPATMDSDALRHAFGKRKGLPELYVLDGQGRVVQKEVGEMLDDDVAALARYGKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="72" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LI71</accession>
    <name>Q1LI71_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="9">ResB-like cytochrome c maturation protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">Rmet_3283</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10155.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517757.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3283"/>
    <dbReference type="EnsemblBacteria" id="ABF10155">
        <property type="protein sequence ID" value="ABF10155"/>
        <property type="gene ID" value="Rmet_3283"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3283"/>
    <dbReference type="eggNOG" id="COG1333">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025116_0_0_4"/>
    <dbReference type="OMA" id="MLYVRER"/>
    <dbReference type="OrthoDB" id="1518243at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017004">
        <property type="term" value="P:cytochrome complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023494">
        <property type="entry name" value="Cyt_c_bgen_Ccs1/CcsB/ResB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007816">
        <property type="entry name" value="ResB-like_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31566">
        <property type="entry name" value="PTHR31566"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05140">
        <property type="entry name" value="ResB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0201">Cytochrome c-type biogenesis</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="28"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="81"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="175"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="644"/>
            <end position="662"/>
        </location>
    </feature>
    <feature type="domain" description="ResB" evidence="8">
        <location>
            <begin position="28"/>
            <end position="704"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="725"/>
            <end position="750"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="733"/>
            <end position="750"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF05140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ABF10155.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="750" mass="81552" checksum="3B12D09C2C2CCDAB" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="92" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LIH8</accession>
    <name>Q1LIH8_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="7 10 11">Ribulose-phosphate 3-epimerase</fullName>
            <ecNumber evidence="7 10 11">5.1.3.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">cbbE2</name>
        <name evidence="10" type="synonym">rpe</name>
        <name evidence="16" type="ordered locus">Rmet_3176</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Catalyzes the reversible epimerization of D-ribulose 5-phosphate to D-xylulose 5-phosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 10 11">
            <text>D-ribulose 5-phosphate = D-xylulose 5-phosphate</text>
            <dbReference type="Rhea" id="RHEA:13677"/>
            <dbReference type="ChEBI" id="CHEBI:57737"/>
            <dbReference type="ChEBI" id="CHEBI:58121"/>
            <dbReference type="EC" id="5.1.3.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Co(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:48828"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="4">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10 13">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
        <text evidence="10 13">Binds 1 divalent metal cation per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="10">Carbohydrate degradation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6 10 11">Belongs to the ribulose-phosphate 3-epimerase family.</text>
    </comment>
    <dbReference type="EC" id="5.1.3.1" evidence="7 10 11"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10048.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3176"/>
    <dbReference type="EnsemblBacteria" id="ABF10048">
        <property type="protein sequence ID" value="ABF10048"/>
        <property type="gene ID" value="Rmet_3176"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3176"/>
    <dbReference type="eggNOG" id="COG0036">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_054856_1_0_4"/>
    <dbReference type="OMA" id="WLQVDGG"/>
    <dbReference type="UniPathway" id="UPA00705"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004750">
        <property type="term" value="F:D-ribulose-phosphate 3-epimerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019323">
        <property type="term" value="P:pentose catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006098">
        <property type="term" value="P:pentose-phosphate shunt"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00429">
        <property type="entry name" value="RPE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02227">
        <property type="entry name" value="RPE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026019">
        <property type="entry name" value="Ribul_P_3_epim"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000056">
        <property type="entry name" value="Ribul_P_3_epim-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011060">
        <property type="entry name" value="RibuloseP-bd_barrel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11749">
        <property type="entry name" value="PTHR11749"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00834">
        <property type="entry name" value="Ribul_P_3_epim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001461">
        <property type="entry name" value="RPE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51366">
        <property type="entry name" value="SSF51366"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01163">
        <property type="entry name" value="rpe"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01085">
        <property type="entry name" value="RIBUL_P_3_EPIMER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01086">
        <property type="entry name" value="RIBUL_P_3_EPIMER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 11" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="13" id="KW-0170">Cobalt</keyword>
    <keyword evidence="9 10 11" id="KW-0413">Isomerase</keyword>
    <keyword evidence="13" id="KW-0464">Manganese</keyword>
    <keyword evidence="8 10 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13" id="KW-0862">Zinc</keyword>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="10 14">
        <location>
            <begin position="178"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="10">
        <location>
            <begin position="215"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate binding" evidence="10 14">
        <location>
            <begin position="237"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="10 12">
        <location>
            <position position="71"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="10 12">
        <location>
            <position position="215"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10 14">
        <location>
            <position position="44"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10 13">
        <location>
            <position position="69"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10 13">
        <location>
            <position position="71"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10 13 14">
        <location>
            <position position="102"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10 13">
        <location>
            <position position="215"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="217"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00001941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00001954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00009541"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00013188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02227"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRNR" id="PIRNR001461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR001461-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR001461-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR001461-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="ABF10048.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="266" mass="28659" checksum="6B9F4C58004FD735" modified="2006-05-30" version="1">MAGASGPARRSSRLRGPDRRKIPPIPPSARPNIMSSPTFRIAPSILSADFARLGEEVRRVTEAGADWIHFDVMDNHYVPNLTVGPLVCEAIRPHVTAPIDVHLMVRPVDRIIPDFARAGANIITFHPEASDHIDRSLALIRDNGCQAGLVFNPATPLHYLDHVMDRLDVILLMSVNPGFGGQSFIPETLNKLRAVRQRIDAYTARTGRRILLEVDGGVKVDNIAEIAAAGADTFVAGSAVFGKPDADGGYKGVIGALRAELATVGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="106" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LIP3</accession>
    <name>Q1LIP3_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Dephospho-CoA kinase</fullName>
            <ecNumber evidence="2">2.7.1.24</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">Dephosphocoenzyme A kinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="2 3" type="primary">coaE</name>
        <name evidence="3" type="ordered locus">Rmet_3111</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Catalyzes the phosphorylation of the 3'-hydroxyl group of dephosphocoenzyme A to form coenzyme A.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>3'-dephospho-CoA + ATP = ADP + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:18245"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57328"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.24"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Cofactor biosynthesis; coenzyme A biosynthesis; CoA from (R)-pantothenate: step 5/5.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the CoaE family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.24" evidence="2"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09983.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517607.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3111"/>
    <dbReference type="EnsemblBacteria" id="ABF09983">
        <property type="protein sequence ID" value="ABF09983"/>
        <property type="gene ID" value="Rmet_3111"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3111"/>
    <dbReference type="eggNOG" id="COG0237">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_057180_1_2_4"/>
    <dbReference type="OMA" id="QMDIEQK"/>
    <dbReference type="OrthoDB" id="1515383at2"/>
    <dbReference type="UniPathway" id="UPA00241">
        <property type="reaction ID" value="UER00356"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004140">
        <property type="term" value="F:dephospho-CoA kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015937">
        <property type="term" value="P:coenzyme A biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00376">
        <property type="entry name" value="Dephospho_CoA_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001977">
        <property type="entry name" value="Depp_CoAkinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01121">
        <property type="entry name" value="CoaE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00152">
        <property type="entry name" value="TIGR00152"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51219">
        <property type="entry name" value="DPCK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0173">Coenzyme A biosynthesis</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 3" id="KW-0418">Kinase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0808">Transferase</keyword>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="11"/>
            <end position="16"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00376"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF09983.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="214" mass="22883" checksum="E662EB6A8C4C4B57" modified="2006-05-30" version="1">MLEIGLTGGIGSGKTRVADMFAARGAALIDTDLIAHEITAPGGPAIAPLVAAFGPGCLRQDGAMDRDAMRALVFSDPSAKTRLEGITHPLIRQVTEARATAIREAGHNPYLIYVVPLLVESSTWRARVGRVLVVDCQEETQIARVMARNGFTRDQVLAIMRRQATRAERMAVADDLIDNDGPPEALNAQVAKLDALYRSLSDTSGNTSENAAHP</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="57" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LIX1</accession>
    <name>Q1LIX1_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_3032</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09905.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_003056207.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3032"/>
    <dbReference type="EnsemblBacteria" id="ABF09905">
        <property type="protein sequence ID" value="ABF09905"/>
        <property type="gene ID" value="Rmet_3032"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3032"/>
    <dbReference type="eggNOG" id="ENOG5032S4W">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_187484_0_0_4"/>
    <dbReference type="OMA" id="MWWIIET"/>
    <dbReference type="OrthoDB" id="1911865at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF09905.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="69" mass="7857" checksum="A6547303B2BA87E0" modified="2006-05-30" version="1">MTRTTTNRPRMAAVYAPGTVRARRWHGDGDVRGYRPPSGWSARADLTDIHPITGRALPRAVWWIIETKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="56" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LIX6</accession>
    <name>Q1LIX6_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Lipoprotein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_3027</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09900.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_003056199.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_3027"/>
    <dbReference type="EnsemblBacteria" id="ABF09900">
        <property type="protein sequence ID" value="ABF09900"/>
        <property type="gene ID" value="Rmet_3027"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_3027"/>
    <dbReference type="eggNOG" id="COG3616">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_119528_1_1_4"/>
    <dbReference type="OMA" id="RMCLLAN"/>
    <dbReference type="OrthoDB" id="1558145at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021341">
        <property type="entry name" value="DUF2958"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11171">
        <property type="entry name" value="DUF2958"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF09900.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="115" mass="12744" checksum="10567E8763880D05" modified="2006-05-30" version="1">MNQLMTEDERKQLLEHGRVRAAGRAIDPLPVVRLFTPDAHATWLLVSLDPADGDTAHGLIDLGISMPELGEIKLSDLASIVGPSKQPVMRDRYFRAVRPLSEYLRLAQENGSVLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="59" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LJ42</accession>
    <name>Q1LJ42_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_2961</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09834.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517497.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF09834">
        <property type="protein sequence ID" value="ABF09834"/>
        <property type="gene ID" value="Rmet_2961"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2961"/>
    <dbReference type="HOGENOM" id="CLU_2069179_0_0_4"/>
    <dbReference type="OMA" id="YRHGETT"/>
    <dbReference type="OrthoDB" id="2026753at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF09834.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="106" mass="11884" checksum="B71BCC2EFCDAD131" modified="2006-05-30" version="1">MRQSSNLAVAYASVPRRRIASNVQWIRLRLAVSSAQVFMLRQTLHRAIGNLARVYVVEVDHRHGETTLHVEVEREDREAAMHAIMAALPAAEFGVTTMLGDDHATH</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LJ49</accession>
    <name>Q1LJ49_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized YceI-like periplasmic protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_2954</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09827.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008641904.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2954"/>
    <dbReference type="EnsemblBacteria" id="ABF09827">
        <property type="protein sequence ID" value="ABF09827"/>
        <property type="gene ID" value="Rmet_2954"/>
    </dbReference>
    <dbReference type="GeneID" id="60820609"/>
    <dbReference type="KEGG" id="rme:Rmet_2954"/>
    <dbReference type="eggNOG" id="COG2353">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_071003_5_1_4"/>
    <dbReference type="OMA" id="FPQATFQ"/>
    <dbReference type="OrthoDB" id="1767227at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.128.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007372">
        <property type="entry name" value="Lipid/polyisoprenoid-bd_YceI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036761">
        <property type="entry name" value="TTHA0802/YceI-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04264">
        <property type="entry name" value="YceI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00867">
        <property type="entry name" value="YceI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101874">
        <property type="entry name" value="SSF101874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193600" evidence="1">
        <location>
            <begin position="29"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="domain" description="YceI" evidence="2">
        <location>
            <begin position="27"/>
            <end position="187"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00867"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF09827.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="191" mass="20301" checksum="28E5D5CD8A248BBD" modified="2006-05-30" version="1">MKRMSRPGVMLVSAALVTAGLAANIGWAQIDTAKSSVTATARQIGVPMEGKFKKFDATVDFDPAKLATSAAKVEIDVSSFEIGDAETTKEVKGREWFDAAKFPKAVFQSTSIKAGAAGKYDVAGKLTIKGKTVDVVVPASYKQDAGGQVFEGVLPIKRTVFNIGDGEWKDTSVVADDVQIKFRIVTPVKKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LJ87</accession>
    <name>Q1LJ87_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Deoxynucleoside kinase</fullName>
            <ecNumber evidence="3">2.7.1.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_2916</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.7.1.-" evidence="3"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09789.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517462.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2916"/>
    <dbReference type="EnsemblBacteria" id="ABF09789">
        <property type="protein sequence ID" value="ABF09789"/>
        <property type="gene ID" value="Rmet_2916"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2916"/>
    <dbReference type="eggNOG" id="COG1428">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030466_2_0_4"/>
    <dbReference type="OMA" id="EAMVMTP"/>
    <dbReference type="OrthoDB" id="1275854at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019136">
        <property type="term" value="F:deoxynucleoside kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01673">
        <property type="entry name" value="dNK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002624">
        <property type="entry name" value="DCK/DGK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031314">
        <property type="entry name" value="DNK_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01712">
        <property type="entry name" value="dNK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000705">
        <property type="entry name" value="DNK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="dNK" evidence="2">
        <location>
            <begin position="8"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="138"/>
            <end position="142"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRSR" id="PIRSR000705-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01712"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF09789.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="213" mass="24210" checksum="4D03AC0AB55D4292" modified="2006-05-30" version="1">MLDHLRRIVIEGPVGSGKAALAQRLARHLQAAELPDGARNNPFLERFYREPQRYALAMQLACLNQRVTQLQQWHSAMLAGQRMIGNFLFARDRVYASLTLEADELALYDALAARLQAPAQRVDLVIMLQATPSLLRERIARRGLPGESGIDEQYLQRLTDAYGELFHRYDEAPVLIVDTAHFNPVDNDVDFRTLLSRIENMRGRRAFLNLVAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LJK8</accession>
    <name>Q1LJK8_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Enoyl-CoA hydratase/isomerase</fullName>
            <ecNumber evidence="3">4.2.1.17</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_2795</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the enoyl-CoA hydratase/isomerase family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.17" evidence="3"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09668.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517363.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2795"/>
    <dbReference type="EnsemblBacteria" id="ABF09668">
        <property type="protein sequence ID" value="ABF09668"/>
        <property type="gene ID" value="Rmet_2795"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2795"/>
    <dbReference type="eggNOG" id="COG1024">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009834_7_3_4"/>
    <dbReference type="OMA" id="LTQALMC"/>
    <dbReference type="OrthoDB" id="1498685at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004300">
        <property type="term" value="F:enoyl-CoA hydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016853">
        <property type="term" value="F:isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.12.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029045">
        <property type="entry name" value="ClpP/crotonase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001753">
        <property type="entry name" value="Enoyl-CoA_hydra/iso"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014748">
        <property type="entry name" value="Enoyl-CoA_hydra_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00378">
        <property type="entry name" value="ECH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52096">
        <property type="entry name" value="SSF52096"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0413">Isomerase</keyword>
    <keyword evidence="3" id="KW-0456">Lyase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF09668.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="260" mass="27385" checksum="AD9A2B4446111344" modified="2006-05-30" version="1">MSDTVTFSREGSTAVVTLSNPGKLNAISVDMWQALAAGFATLAADMSLRCVLVRGADGNFAAGADIAEFPTVRGDEAAVRRYHTEIIAPALRAISECPHPTLAAIEGVCVGGGLEIACNCDLRVAAAGSRFGVPINRLGFPMAPGELRGLLALVGRAVTLEILLEGRVFDALEAERKGLLTRVVPAAAMHDEVTATVQRLSAGAPLAARINKTTIRRLSPDPTLLTDAEFDAHFRYATSRDHAEGVAAFLAHRPPDFTGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="69" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LJS2</accession>
    <name>Q1LJS2_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Polysaccharide biosynthesis protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_2731</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09604.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517305.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2731"/>
    <dbReference type="EnsemblBacteria" id="ABF09604">
        <property type="protein sequence ID" value="ABF09604"/>
        <property type="gene ID" value="Rmet_2731"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2731"/>
    <dbReference type="eggNOG" id="COG2244">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_040633_0_0_4"/>
    <dbReference type="OMA" id="EAFGFVH"/>
    <dbReference type="OrthoDB" id="564091at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="40"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="79"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="124"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="155"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="180"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="244"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="269"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="308"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="339"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="378"/>
            <end position="402"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF09604.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="419" mass="44571" checksum="545E54D094787AD6" modified="2006-05-30" version="1">MRASHISWNLFGLGLPLLIAAVTVPHLLSGLGSQRFGLLALAWGLIGYAGALDLGIGRATTQRVAALRSGGQEAVIPHVVATATRLTLVTGGIGMVLIIVSAILGVGELVHANDVPQAEIRSAVLMLGLALPMQAVSATYRGVSEAYLSFRNISVLRILLGAANFGIPYLIALFSTRVDLLIASLVVSRAIALWFYRIFALQCLSSGDTRGTYSRKLAHQLFQFGGWYTVSSILGPLMTQADRFLIAAILGAAAVTTYVIPYEVTVQTLILSGAVTTVAFPAISRMLVNDPDGAALLFRRWLLRVSGAMAIGLIILAIILPGLLTLWLRHPADPASVMVGRILCIGVLANAIGSMYFALLQAKRRTRATAMLHLVEVPLYLCALVALTKLFGVTGAAAAWSLRMCFDASGMKILVRQAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="66" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LJT1</accession>
    <name>Q1LJT1_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative exopolysaccharide chain length determinant protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_2722</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09595.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2722"/>
    <dbReference type="EnsemblBacteria" id="ABF09595">
        <property type="protein sequence ID" value="ABF09595"/>
        <property type="gene ID" value="Rmet_2722"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2722"/>
    <dbReference type="eggNOG" id="COG3206">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1045345_0_0_4"/>
    <dbReference type="OrthoDB" id="1184929at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="23"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="253"/>
            <end position="273"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF09595.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="285" mass="30880" checksum="A6AF04639EC4788E" modified="2006-05-30" version="1">MPIANSNASPDAWRLPPKSIKSIGLAGVIGALCGFAVFHFVPPRWTARITVQVGQISSPEGGAVTSRLVENQLTAVDRYNLPSSRLKVLNELGLPASSESKESRLIFETLLGSPGKGPNLINLQVSAYSREQAMAVMSSSVKLLAAEHQKLFAPSISRMSGDLANLTDKLKAAERDYANSYAWLVANSKQKNDPINSVRDVQLTNLTMLADRQSIELRHRIMQFQEALDPTSSYPTRPMGEIFAPDRPSTPGWTVFVAAGAVFGLALGTLLVVQRLASQARKPDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="62" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LK11</accession>
    <name>Q1LK11_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Putative ATP-ase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_2638</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09515.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517214.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2638"/>
    <dbReference type="EnsemblBacteria" id="ABF09515">
        <property type="protein sequence ID" value="ABF09515"/>
        <property type="gene ID" value="Rmet_2638"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2638"/>
    <dbReference type="eggNOG" id="COG0645">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_108060_1_0_4"/>
    <dbReference type="OMA" id="YRQRRFV"/>
    <dbReference type="OrthoDB" id="1574819at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF09515.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="192" mass="21507" checksum="E2D1A7C66E1D8C96" modified="2006-05-30" version="1">MPRLIFFCGHAGTGKTTLAHKLIGPLMQATGEPFCLLDKDTLYGRYSAAAMRAITGDPNDRDSPAYLDNLRDPEYEGLLDTARENLMLGISVIVVGPLSREIRAHQLTDPSWLRVPEGTQTHVVWVHLPESEAQARIVRRGNPNDAYKLAHWDAYRTRRFLPEPAEYPELIYFDNVAPQEADVNRLLAALAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LK47</accession>
    <name>Q1LK47_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Thiolase</fullName>
            <ecNumber evidence="4">2.3.1.176</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Rmet_2602</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the thiolase-like superfamily. Thiolase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.176" evidence="4"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09479.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011517182.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2602"/>
    <dbReference type="EnsemblBacteria" id="ABF09479">
        <property type="protein sequence ID" value="ABF09479"/>
        <property type="gene ID" value="Rmet_2602"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2602"/>
    <dbReference type="eggNOG" id="COG0183">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035425_4_0_4"/>
    <dbReference type="OMA" id="FEGKTAM"/>
    <dbReference type="OrthoDB" id="311458at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033814">
        <property type="term" value="F:propanoyl-CoA C-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050632">
        <property type="term" value="F:propionyl-CoA C2-trimethyltridecanoyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.47.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002155">
        <property type="entry name" value="Thiolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016039">
        <property type="entry name" value="Thiolase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020617">
        <property type="entry name" value="Thiolase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020616">
        <property type="entry name" value="Thiolase_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02803">
        <property type="entry name" value="Thiolase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00108">
        <property type="entry name" value="Thiolase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000429">
        <property type="entry name" value="Ac-CoA_Ac_transf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53901">
        <property type="entry name" value="SSF53901"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 4" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Thiolase_N" evidence="2">
        <location>
            <begin position="24"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="domain" description="Thiolase_C" evidence="3">
        <location>
            <begin position="260"/>
            <end position="350"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU003557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF02803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF09479.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="382" mass="39918" checksum="9483529AF9F93725" modified="2006-05-30" version="1">MNRLLQQTSVYVIGVGLHPYQFASDTPYVSLGLRAMREALSDAGIAWPEVQSAYVGTSAIGMATGRVMFRHLGSTGLAVTQVESASASGCSAFRQACLEVASGVSDVVVAIGVDKFGDGRKAADKDGLPHLAPTATMPPVKFALMAHEYLRQHGLKPEAMARVAVKNHGNAALNPYAQFRKARTLEQVMASPKVVGDLTVQQCCPRGDGAAAVIVVSEAAIKRLGLDKRRAVRVLASVANSEELEHGDSSAAVDMVRKSTAMAYEQAGVGPRDLNLVELHEAFSIEELVYTEAMDLCAPGEGAAWLERGASAIGGECAVNASGGLLGMGHPTGPTGIGQIAEITRQIRGEAHGRQHPGARLGLAHMIGLGSVAFAHILAASH</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="56" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LL33</accession>
    <name>Q1LL33_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_2264</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09143.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2264"/>
    <dbReference type="EnsemblBacteria" id="ABF09143">
        <property type="protein sequence ID" value="ABF09143"/>
        <property type="gene ID" value="Rmet_2264"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2264"/>
    <dbReference type="eggNOG" id="ENOG5032XZ6">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_697961_0_0_4"/>
    <dbReference type="OMA" id="ISEDFWA"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="47"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="73"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="102"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="141"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="180"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="220"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="259"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="331"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="369"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="399"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="421"/>
            <end position="440"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF09143.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="456" mass="50298" checksum="A81823BA9D2FD1F6" modified="2006-05-30" version="1">MDMSGTVYPNSASQQHDVTANVHRCAYCDNLDCYNAARTKPQTPLKMLFACVLMALVGLLISVKIGPGRGNPFTAFFCVWSAVTVGAFLAEDTFLTISEDFWAMLGAFLSIYLGCALYSSLRARVLPVESHISTISYRDKFVDIGQIVSVIALPIIYLKASEIAEMSVFSPAGYMALRAAFIAEVAASYGPIGYIVPVSFVIASIRLTQFLDKQTNKKNLAIAIAVALCMAYLTTGRTFFMMLFCFLLFPLIFRGKIKLGGVVIAGVVLAASFVMIALLTQRGVSATASVDDNVDGIIKTLRVYFLSPIFAMGSVFDGNGSATYGDYTFRFFYMLANVIGLNIEIPPLIRDYVLIPDMTNVFTVMDPYYRDFGVGGVLIFAALSGLAHDALYEKAKSEGGPYIFIHAAFMFPLVMQFFQDMYMSLLSTWIQVVFWYMLFVRVNSGKKNQQEIPQNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="113" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LLG2</accession>
    <name>Q1LLG2_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Transcription termination factor Rho</fullName>
            <ecNumber evidence="8">3.6.4.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">ATP-dependent helicase Rho</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8 11" type="primary">rho</name>
        <name evidence="11" type="ordered locus">Rmet_2135</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Facilitates transcription termination by a mechanism that involves Rho binding to the nascent RNA, activation of Rho's RNA-dependent ATPase activity, and release of the mRNA from the DNA template.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Homohexamer. The homohexamer assembles into an open ring structure.</text>
    </comment>
    <comment type="similarity">
        <text evidence="8 9">Belongs to the Rho family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.-" evidence="8"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF09014.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008647942.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2135"/>
    <dbReference type="EnsemblBacteria" id="ABF09014">
        <property type="protein sequence ID" value="ABF09014"/>
        <property type="gene ID" value="Rmet_2135"/>
    </dbReference>
    <dbReference type="GeneID" id="60821283"/>
    <dbReference type="KEGG" id="rme:Rmet_2135"/>
    <dbReference type="eggNOG" id="COG1158">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016377_4_3_4"/>
    <dbReference type="OMA" id="TRLCRAH"/>
    <dbReference type="OrthoDB" id="1619125at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008186">
        <property type="term" value="F:ATP-dependent activity, acting on RNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006353">
        <property type="term" value="P:DNA-templated transcription, termination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd04459">
        <property type="entry name" value="Rho_CSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01128">
        <property type="entry name" value="rho_factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01884">
        <property type="entry name" value="Rho"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000194">
        <property type="entry name" value="ATPase_F1/V1/A1_a/bsu_nucl-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011129">
        <property type="entry name" value="CSD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041703">
        <property type="entry name" value="Rho_factor_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011112">
        <property type="entry name" value="Rho_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036269">
        <property type="entry name" value="Rho_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011113">
        <property type="entry name" value="Rho_RNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004665">
        <property type="entry name" value="Term_rho"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46425">
        <property type="entry name" value="PTHR46425"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00006">
        <property type="entry name" value="ATP-synt_ab"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07498">
        <property type="entry name" value="Rho_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07497">
        <property type="entry name" value="Rho_RNA_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00357">
        <property type="entry name" value="CSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00959">
        <property type="entry name" value="Rho_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF68912">
        <property type="entry name" value="SSF68912"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00767">
        <property type="entry name" value="rho"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51856">
        <property type="entry name" value="RHO_RNA_BD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 8" id="KW-0347">Helicase</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8 9" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="7 8" id="KW-0804">Transcription</keyword>
    <keyword evidence="6 8" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="1 8" id="KW-0806">Transcription termination</keyword>
    <feature type="domain" description="Rho RNA-BD" evidence="10">
        <location>
            <begin position="48"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="169"/>
            <end position="174"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="181"/>
            <end position="186"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="212"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51856"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ABF09014.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="420" mass="47330" checksum="EC5F71897B8F4DA0" modified="2006-05-30" version="1">MHLTELKSLHVSALLEMAATLEIDNAQRMRKQELMFAILKKRAKMGETIYGDGTLEVLPDGFGFLRSPETSYLASTDDIYISPSQIRRFNLHTGDSIEGEVRTPKDGERYFALVKVDKVNGQPPEAVKNRIMFENLTPLHPNRPLTLERDIRAEENITGRIVDIIAPIGRGQRALLVASPKSGKTVMLQHIAHAIANNHPEADLFVLLIDERPEEVTEMQRSVRGEVVASTFDEPAIRHVQVAEMVIEKAKRLVELKRDVVILLDSITRLARAYNTVVPASGKVLTGGVDANALQRPKRFFGAARNLEEGGSLTIIATALIETGSRMDDVIYEEFKGTGNMEVHLERRLAEKRVYPAINLNKSGTRREELLIKPDILQKIWILRKFISDMDEVQAMEFILDKMKPTKNNAEFFDLMRRGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="47" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LLT0</accession>
    <name>Q1LLT0_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_2017</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08896.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_2017"/>
    <dbReference type="EnsemblBacteria" id="ABF08896">
        <property type="protein sequence ID" value="ABF08896"/>
        <property type="gene ID" value="Rmet_2017"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_2017"/>
    <dbReference type="eggNOG" id="COG0457">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_050666_1_0_4"/>
    <dbReference type="OMA" id="AKCEDEF"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021352">
        <property type="entry name" value="DUF2971"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11185">
        <property type="entry name" value="DUF2971"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF08896.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="315" mass="36513" checksum="5EBFE5B814F29A46" modified="2006-05-30" version="1">MPQDMASAVWQNCSPAPSQFDMPDIEDTTPPPVLYKFMSINGERWTWFQNLIRLGQIYLSSPLDFNDPFDCLPRLDIPSNQDKRVNKRLFRAGARQGHSRKVRREQVGIVRSQSSSVWRENYQNSAHRRIREVGVYCVSANNSHPLMWSHYAEKHTGLSIGFKTGVGIFRIAHKIEYSDIRPHYNHLSTNSKEFYDVYHKKAKFWEYEDEYRILSLNTGIDPKSLMRGIEDDADLMRFIGRPAGHGPATLSPSVIASITFGCNMPPNERTRIVDFVRAQGLNVDFFEAVLDEHKFALSIRPFHIEQGRVRNRRRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="57" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LLV2</accession>
    <name>Q1LLV2_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_1995</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08874.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516719.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1995"/>
    <dbReference type="EnsemblBacteria" id="ABF08874">
        <property type="protein sequence ID" value="ABF08874"/>
        <property type="gene ID" value="Rmet_1995"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1995"/>
    <dbReference type="eggNOG" id="COG3431">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_113758_2_1_4"/>
    <dbReference type="OrthoDB" id="1942205at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020948">
        <property type="entry name" value="P_starv_induced_PsiE-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06146">
        <property type="entry name" value="PsiE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="20"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="62"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="92"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="122"/>
            <end position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF08874.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="148" mass="16376" checksum="5B305020220A4DD1" modified="2006-05-30" version="1">MQVEKKITAKITFWFEQSILVAAQILLMAVILAMVIQLWIMFSSDILERVSSIDTVPELMKSVQTAFSGVLLIILGLELMETLRVYFRSHRVRLETILAVAIIAVGRHVINLDVEHMSGGSLMGVAAVVLSLTGGYFLVRFKSPPSAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LM04</accession>
    <name>Q1LM04_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Cation/multidrug efflux system, mebrane-fusion component (Modular protein) (RND family)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Rmet_1943</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08822.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1943"/>
    <dbReference type="EnsemblBacteria" id="ABF08822">
        <property type="protein sequence ID" value="ABF08822"/>
        <property type="gene ID" value="Rmet_1943"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1943"/>
    <dbReference type="eggNOG" id="COG0845">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018816_1_0_4"/>
    <dbReference type="OMA" id="PQSRTFA"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032317">
        <property type="entry name" value="HlyD_D23"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006143">
        <property type="entry name" value="RND_pump_MFP"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16576">
        <property type="entry name" value="HlyD_D23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01730">
        <property type="entry name" value="RND_mfp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HlyD_D23" evidence="3">
        <location>
            <begin position="197"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="501"/>
            <end position="523"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF16576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF08822.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="523" mass="55726" checksum="232F424011CF1568" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="95" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LMD0</accession>
    <name>Q1LMD0_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Transcriptional regulator, IclR-family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ordered locus">Rmet_1817</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08696.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516546.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1817"/>
    <dbReference type="EnsemblBacteria" id="ABF08696">
        <property type="protein sequence ID" value="ABF08696"/>
        <property type="gene ID" value="Rmet_1817"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1817"/>
    <dbReference type="eggNOG" id="COG1414">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_062618_0_0_4"/>
    <dbReference type="OMA" id="DIGMPIP"/>
    <dbReference type="OrthoDB" id="370329at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029016">
        <property type="entry name" value="GAF-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014757">
        <property type="entry name" value="Tscrpt_reg_IclR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005471">
        <property type="entry name" value="Tscrpt_reg_IclR_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09339">
        <property type="entry name" value="HTH_IclR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01614">
        <property type="entry name" value="IclR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00346">
        <property type="entry name" value="HTH_ICLR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51077">
        <property type="entry name" value="HTH_ICLR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51078">
        <property type="entry name" value="ICLR_ED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH iclR-type" evidence="3">
        <location>
            <begin position="13"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="domain" description="IclR-ED" evidence="4">
        <location>
            <begin position="76"/>
            <end position="259"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABF08696.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="260" mass="28762" checksum="4AF90F74E73BA552" modified="2006-05-30" version="1">MPTRPHHPDPAFATTLAHGLALLQCFRVGETVLGNKELADRTGLSKATISRLTYTLAARGLLLYDTHLRRYRLGSTALSLGYPLLASLRVRQLARPLMKALADQVGGSVSLGLRDRLRMVYVETSRGHDAIAFRPDIGASLPMLATAMGRAWLAWAPEDVRHRLLDALREAEPDDYERLAGAVDTAIEELATLGFTRSRGDWQRDVHAVAVPMRTPIDGEILVFNCGVPAVRMTPRKLEREMGPRLLKLVAQVETLVAWE</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LMG3</accession>
    <name>Q1LMG3_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Phenol hydroxylase P3 protein (Phenol 2-monooxygenase P3 component)</fullName>
            <ecNumber evidence="3">1.14.13.25</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">phyC</name>
        <name evidence="3" type="ordered locus">Rmet_1784</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EC" id="1.14.13.25" evidence="3"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08663.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008643949.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1784"/>
    <dbReference type="EnsemblBacteria" id="ABF08663">
        <property type="protein sequence ID" value="ABF08663"/>
        <property type="gene ID" value="Rmet_1784"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1784"/>
    <dbReference type="eggNOG" id="COG3350">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_040795_0_0_4"/>
    <dbReference type="OMA" id="FMPFMSG"/>
    <dbReference type="OrthoDB" id="147428at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106317">
        <property type="term" value="F:methane monooxygenase NADH activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106318">
        <property type="term" value="F:methane monooxygenase NADPH activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006725">
        <property type="term" value="P:cellular aromatic compound metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.620.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009078">
        <property type="entry name" value="Ferritin-like_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003430">
        <property type="entry name" value="Phenol_Hydrox"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012348">
        <property type="entry name" value="RNR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007029">
        <property type="entry name" value="YHS_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02332">
        <property type="entry name" value="Phenol_Hydrox"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04945">
        <property type="entry name" value="YHS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47240">
        <property type="entry name" value="SSF47240"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="YHS" evidence="2">
        <location>
            <begin position="420"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="493"/>
            <end position="515"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF04945"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF08663.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="515" mass="60237" checksum="E5945A37EC2BB794" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LMH0</accession>
    <name>Q1LMH0_CUPMC</name>
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        <submittedName>
            <fullName evidence="6">Putative Na/Pi cotransporter II-related</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ordered locus">Rmet_1777</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08656.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF08656">
        <property type="protein sequence ID" value="ABF08656"/>
        <property type="gene ID" value="Rmet_1777"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1777"/>
    <dbReference type="eggNOG" id="COG1283">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025623_2_0_4"/>
    <dbReference type="OMA" id="TWACHSS"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015114">
        <property type="term" value="F:phosphate ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005436">
        <property type="term" value="F:sodium:phosphate symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044341">
        <property type="term" value="P:sodium-dependent phosphate transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.220">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003841">
        <property type="entry name" value="Na/Pi_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030147">
        <property type="entry name" value="NptA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038078">
        <property type="entry name" value="PhoU-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10010">
        <property type="entry name" value="PTHR10010"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10010:SF46">
        <property type="entry name" value="PTHR10010:SF46"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02690">
        <property type="entry name" value="Na_Pi_cotrans"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
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        <location>
            <begin position="31"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="81"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="130"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="163"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="204"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="242"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="267"/>
            <end position="289"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABF08656.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="600" mass="63212" checksum="30747AB232BEAEA5" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="95" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LMJ0</accession>
    <name>Q1LMJ0_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Transcriptional regulator, LysR family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_1757</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
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        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the LysR transcriptional regulatory family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08636.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1757"/>
    <dbReference type="EnsemblBacteria" id="ABF08636">
        <property type="protein sequence ID" value="ABF08636"/>
        <property type="gene ID" value="Rmet_1757"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1757"/>
    <dbReference type="eggNOG" id="COG0583">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039613_16_2_4"/>
    <dbReference type="OMA" id="IRHELMA"/>
    <dbReference type="OrthoDB" id="1661500at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
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    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005119">
        <property type="entry name" value="LysR_subst-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000847">
        <property type="entry name" value="Tscrpt_reg_HTH_LysR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00126">
        <property type="entry name" value="HTH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03466">
        <property type="entry name" value="LysR_substrate"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50931">
        <property type="entry name" value="HTH_LYSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH lysR-type" evidence="2">
        <location>
            <begin position="1"/>
            <end position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF08636.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="297" mass="32870" checksum="B03572D20BD14833" modified="2006-05-30" version="1">MDLNALALLVEIIDAGNLSRAANQLGMSRANVSKRLNHFERQLGAELLRRSTRQLEPTELGWQLYEHGRAIRHELMAAQESVQSLGKRLSGTVRLSVPSGFGQHTMSAWFIEFMTMYPAITLNVIFDNDIEDLIKGAVDFSIRVMAETPANAVAHSLGDVEYVACVTPALVSQYGMPHTLDQFRDLPLITSELTGQKLHAAGRAGAAGGTRPIRPRLMSANFFFLRDAILQGLGVGLVPRYMVERDLAAGTLLQLPLPPQDLAFLRTTMYLLYMPARYQTRAFTTLVAFLRDKAAGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="66" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LMZ9</accession>
    <name>Q1LMZ9_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Rmet_1594</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the DoxX family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08477.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008649650.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1594"/>
    <dbReference type="EnsemblBacteria" id="ABF08477">
        <property type="protein sequence ID" value="ABF08477"/>
        <property type="gene ID" value="Rmet_1594"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1594"/>
    <dbReference type="eggNOG" id="COG2259">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_058421_6_3_4"/>
    <dbReference type="OMA" id="HGGYALE"/>
    <dbReference type="OrthoDB" id="2025630at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032808">
        <property type="entry name" value="DoxX"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07681">
        <property type="entry name" value="DoxX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="13"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="53"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="75"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="111"/>
            <end position="129"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABF08477.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="137" mass="14365" checksum="05DC76238A49CFF0" modified="2006-05-30" version="1">MSHSQGSQDLGKAVLRIVLGVLILLHGISKIMAGPGFVVKVVTDAGLPVELSYLVYVGEVLAPILLIVGLWTRAAALIVVVNMLFAFGLVHTKQLMEMAPTGGWALELQGMYLAGALAVALLGAGRLSFGGIDGRWN</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LN13</accession>
    <name>Q1LN13_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">MOSC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_1580</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08463.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516315.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1580"/>
    <dbReference type="EnsemblBacteria" id="ABF08463">
        <property type="protein sequence ID" value="ABF08463"/>
        <property type="gene ID" value="Rmet_1580"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1580"/>
    <dbReference type="eggNOG" id="COG2258">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_082566_1_1_4"/>
    <dbReference type="OMA" id="CWKLNDR"/>
    <dbReference type="OrthoDB" id="1643056at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030151">
        <property type="term" value="F:molybdenum ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005302">
        <property type="entry name" value="MoCF_Sase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011037">
        <property type="entry name" value="Pyrv_Knase-like_insert_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005163">
        <property type="entry name" value="YiiM-like_3-alpha_domain"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03475">
        <property type="entry name" value="3-alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03473">
        <property type="entry name" value="MOSC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50800">
        <property type="entry name" value="SSF50800"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51340">
        <property type="entry name" value="MOSC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MOSC" evidence="1">
        <location>
            <begin position="32"/>
            <end position="173"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51340"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF08463.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="235" mass="25939" checksum="13F6ED4CFE132D5E" modified="2006-05-30" version="1">MEAVINIPIAAVLAGRVKPFGPKGVPSGIEKTVVSGSVRITKLGLDIDEQGDPKHHGGPEKALHHYPRDHYPVWHDTLAAHGGNVDVLDAPGAFGENFSTHGLTEAEVCIGDRFRVGTALVEVSQARQPCWKLNHRFGYAAMSRSVQERGRTGWYYRVIEEGEVSAGDRIVLVARPYPQWTLQRLLHVLYVDRLNDTALAEMADLAPLAESWRKLARQRVASHVVEDMEKRLAGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="116" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LNF1</accession>
    <name>Q1LNF1_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Zinc metalloprotease</fullName>
            <ecNumber evidence="9">3.4.24.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">yaeL</name>
        <name evidence="11" type="ordered locus">Rmet_1442</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 9">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the peptidase M50B family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="3.4.24.-" evidence="9"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08325.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516192.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1442"/>
    <dbReference type="EnsemblBacteria" id="ABF08325">
        <property type="protein sequence ID" value="ABF08325"/>
        <property type="gene ID" value="Rmet_1442"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1442"/>
    <dbReference type="eggNOG" id="COG0750">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025778_0_2_4"/>
    <dbReference type="OMA" id="QYMVGFG"/>
    <dbReference type="OrthoDB" id="1395197at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001478">
        <property type="entry name" value="PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041489">
        <property type="entry name" value="PDZ_6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004387">
        <property type="entry name" value="Pept_M50_Zn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008915">
        <property type="entry name" value="Peptidase_M50"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42837">
        <property type="entry name" value="PTHR42837"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17820">
        <property type="entry name" value="PDZ_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02163">
        <property type="entry name" value="Peptidase_M50"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00228">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="SSF50156"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00054">
        <property type="entry name" value="TIGR00054"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50106">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9 11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="9" id="KW-0645">Protease</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6 9" id="KW-0862">Zinc</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="102"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="domain" description="PDZ" evidence="10">
        <location>
            <begin position="200"/>
            <end position="297"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU362031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ABF08325.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="463" mass="49234" checksum="7F93C34F0D28DDD8" modified="2006-05-30" version="1">MQTVIAFVVALCILIFVHEMGHYLAARACGVKVLRFSIGFGRPLVRWVGKGRDKTEWTLAAIPLGGYVKMLDERERDPETDPPIDPAELPRAFNRQPVGKRFVVVAAGPLANFLLAVVLYVVLFAGGMREPVPVVAAPAAGTLAAQAGVRDGDRVLSLTANDHTEAVRSWNDLRMAVFSQGFGDARAVLRVRGIDGAERDVALARLPNTGGDPEQDPLAVLGLALKGGPVTITEIVPDSAAQRAGLKAGDRVVAWDGQPLTQASALIRGVRARPGQEVTLGIERAGERIDVKAKLDAAPAPEGEARGGSQVGKLGAALNQSVQTEIVRYPLVQAVARAADQVWNTSALSLKLLGKMLVGQASLQNLSGPLTVADYAGRAANMGWQPFISFLALVSVSLGVLNLLPIPVLDGGHLLYYCVEFLTGRPVPDHWQAMLQKVGIACILLLTSLALFNDVSRLFLARG</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="101" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LNG7</accession>
    <name>Q1LNG7_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Chromosome partition protein Smc</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3 5" type="primary">smc</name>
        <name evidence="5" type="ordered locus">Rmet_1426</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Required for chromosome condensation and partitioning.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="3">Contains large globular domains required for ATP hydrolysis at each terminus and a third globular domain forming a flexible hinge near the middle of the molecule. These domains are separated by coiled-coil structures.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the SMC family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08309.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011516177.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1426"/>
    <dbReference type="EnsemblBacteria" id="ABF08309">
        <property type="protein sequence ID" value="ABF08309"/>
        <property type="gene ID" value="Rmet_1426"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1426"/>
    <dbReference type="eggNOG" id="COG1196">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001042_2_2_4"/>
    <dbReference type="OMA" id="HNKIAME"/>
    <dbReference type="OrthoDB" id="1149850at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030261">
        <property type="term" value="P:chromosome condensation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007062">
        <property type="term" value="P:sister chromatid cohesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01894">
        <property type="entry name" value="Smc_prok"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003395">
        <property type="entry name" value="RecF/RecN/SMC_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024704">
        <property type="entry name" value="SMC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010935">
        <property type="entry name" value="SMC_hinge"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036277">
        <property type="entry name" value="SMC_hinge_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011890">
        <property type="entry name" value="SMC_prok"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06470">
        <property type="entry name" value="SMC_hinge"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02463">
        <property type="entry name" value="SMC_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005719">
        <property type="entry name" value="SMC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00968">
        <property type="entry name" value="SMC_hinge"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75553">
        <property type="entry name" value="SSF75553"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02168">
        <property type="entry name" value="SMC_prok_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1 3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SMC hinge" evidence="4">
        <location>
            <begin position="518"/>
            <end position="625"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="170"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="314"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="405"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="729"/>
            <end position="756"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="785"/>
            <end position="901"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="32"/>
            <end position="39"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABF08309.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="1171" mass="130432" checksum="D58BF46E03F83A76" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LPD6</accession>
    <name>Q1LPD6_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Putative ferric siderophore receptor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">Rmet_1104</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cell outer membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the TonB-dependent receptor family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07990.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515891.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1104"/>
    <dbReference type="EnsemblBacteria" id="ABF07990">
        <property type="protein sequence ID" value="ABF07990"/>
        <property type="gene ID" value="Rmet_1104"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1104"/>
    <dbReference type="eggNOG" id="COG4773">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008287_22_1_4"/>
    <dbReference type="OMA" id="WGTITTT"/>
    <dbReference type="OrthoDB" id="196825at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009279">
        <property type="term" value="C:cell outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015344">
        <property type="term" value="F:siderophore uptake transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038023">
        <property type="term" value="F:signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.130.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.170.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030149">
        <property type="entry name" value="FcuA/FatA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012910">
        <property type="entry name" value="Plug_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037066">
        <property type="entry name" value="Plug_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039423">
        <property type="entry name" value="TonB-dep_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000531">
        <property type="entry name" value="TonB-dep_rcpt_b-brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036942">
        <property type="entry name" value="TonB_rcpt_b-brl_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010105">
        <property type="entry name" value="TonB_sidphr_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32552">
        <property type="entry name" value="PTHR32552"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32552:SF82">
        <property type="entry name" value="PTHR32552:SF82"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07715">
        <property type="entry name" value="Plug"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00593">
        <property type="entry name" value="TonB_dep_Rec"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01783">
        <property type="entry name" value="TonB-siderophor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0998">Cell outer membrane</keyword>
    <keyword evidence="3 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 9" id="KW-0675">Receptor</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0798">TonB box</keyword>
    <feature type="domain" description="Plug" evidence="8">
        <location>
            <begin position="87"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="domain" description="TonB_dep_Rec" evidence="7">
        <location>
            <begin position="275"/>
            <end position="726"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009810"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003357"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00593"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF07715"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ABF07990.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="728" mass="80563" checksum="24A80423FC9D5B59" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LPE2</accession>
    <name>Q1LPE2_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Cobalamin synthesis protein/P47K</fullName>
            <ecNumber evidence="3">3.6.-.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">cobW</name>
        <name evidence="3" type="ordered locus">Rmet_1098</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SIMIBI class G3E GTPase family. CobW subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.-.-" evidence="3"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07984.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515885.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1098"/>
    <dbReference type="EnsemblBacteria" id="ABF07984">
        <property type="protein sequence ID" value="ABF07984"/>
        <property type="gene ID" value="Rmet_1098"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1098"/>
    <dbReference type="eggNOG" id="COG0523">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017452_2_0_4"/>
    <dbReference type="OMA" id="WSQAGPN"/>
    <dbReference type="OrthoDB" id="1267701at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1220.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011629">
        <property type="entry name" value="Cbl_biosynth_CobW-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036627">
        <property type="entry name" value="CobW-likC_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003495">
        <property type="entry name" value="CobW/HypB/UreG_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02492">
        <property type="entry name" value="cobW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07683">
        <property type="entry name" value="CobW_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00833">
        <property type="entry name" value="CobW_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CobW C-terminal" evidence="2">
        <location>
            <begin position="258"/>
            <end position="373"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF07984.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="397" mass="44375" checksum="C479A7CA1DE3C579" modified="2006-05-30" version="1">MLPAKLPVTVLSGFLGAGKTTLLNHILHNREGRRVAVIVNDMSDVNIDAGLVRDGGANLSRTEETLVEMSNGCICCTLREDLLLEVERLAREGRFDQLVIESTGISEPLPVAETFTFEGEDGRSLNEVAHLDTMVTVVDAFNFLRDYGSRDSLQSRGESLGEEDARTVVDLLIDQVEFCDVLVLNKVDLIADADRERLLAILRALNPRARIEIAEFGRVPLERVLGTGLFDFEEASKAPGWLQEMRGQHVPETEEYGIRSFVYGARRPFHPQRFHAFVAAEWPGVVRSKGFFWLASHPTLAGTWSQAGAVARHGPAGYWWAAVPPERWPDDPESVALIRAKWDDSVGDARQEIVLIGVDMDEFELRRCLDACLLTDDEMAQGPDVWTTWHNPFPDWP</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LPJ1</accession>
    <name>Q1LPJ1_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">TGc domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_1049</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07935.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515841.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_1049"/>
    <dbReference type="EnsemblBacteria" id="ABF07935">
        <property type="protein sequence ID" value="ABF07935"/>
        <property type="gene ID" value="Rmet_1049"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_1049"/>
    <dbReference type="eggNOG" id="COG1305">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008973_1_2_4"/>
    <dbReference type="OMA" id="DHAHIFI"/>
    <dbReference type="OrthoDB" id="670657at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013589">
        <property type="entry name" value="Bac_transglu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002931">
        <property type="entry name" value="Transglutaminase-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08379">
        <property type="entry name" value="Bact_transglu_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01841">
        <property type="entry name" value="Transglut_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00460">
        <property type="entry name" value="TGc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="SSF54001"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TGc" evidence="1">
        <location>
            <begin position="183"/>
            <end position="248"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF07935.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="293" mass="31135" checksum="2B3F7554A980695E" modified="2006-05-30" version="1">MHVARDIPVKYKIHHKTIYRYTEPVRRSVHELRLAPRSGPLQSVQYWTLSAPGNLSEARDGFGNIVHNFTMAGPARTIAIVATGEVEVRIDPAPTGQAGAEAGEGGHGAFQDLPEAGESRVSPLYFLSGTPLTQAPADMQAFAAPYLAAGHEVPALLALADGIAERVQYRANTTDVGTSAVEAFRLGSGVCQDQAQVMVAACRALGIPARYVSGYFYDAAAASLASHAWADICLDADRGLWCSIDVTHRCITDGRHVRLAVGRDYASAAPIRGILEGGAGETMEVDIEITPLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="69" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LQ90</accession>
    <name>Q1LQ90_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative substrate-binding region of ABC-type glycine betaine transport system: QATfamily</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_0800</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07686.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515636.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0800"/>
    <dbReference type="EnsemblBacteria" id="ABF07686">
        <property type="protein sequence ID" value="ABF07686"/>
        <property type="gene ID" value="Rmet_0800"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0800"/>
    <dbReference type="eggNOG" id="COG1732">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038355_1_0_4"/>
    <dbReference type="OMA" id="APMNNTY"/>
    <dbReference type="OrthoDB" id="770506at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043190">
        <property type="term" value="C:ATP-binding cassette (ABC) transporter complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007210">
        <property type="entry name" value="ABC_Gly_betaine_transp_sub-bd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04069">
        <property type="entry name" value="OpuAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="domain" description="OpuAC" evidence="2">
        <location>
            <begin position="45"/>
            <end position="311"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF04069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF07686.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="316" mass="34453" checksum="158A25769A05DBB7" modified="2006-05-30" version="1">MRSNKNRHRAPFSESRLYWLVVALGAAFAAILLTTAPARAQSASDPVRIGGKNFTEQLILSSMTSQYLRAHGVDTTLTAGLGSTLMRQAMESGQLDVVWDYTGTALIVFNKVEEKLDGPQSYARVKALDAAKGLVWLDPSAINNTYALAMPKERAGTATTLSAYAEKMRADGEDKRHPLAVDMEFAARPDGLEPLKAAYGLPFTRKDVIQLDPGLVYTALKNNQVDLGLVYTTDGRVKGFDLVLLEDDLNFFPPYNAVPVVRKAALDAHPELADLLNALAAKLDNAAMTDMNYQVDIDQQPVDKVAGDFLRSHGLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="58" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LQK3</accession>
    <name>Q1LQK3_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Probable lipoprotein transmembrane putative exported protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_0687</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07573.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515540.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0687"/>
    <dbReference type="EnsemblBacteria" id="ABF07573">
        <property type="protein sequence ID" value="ABF07573"/>
        <property type="gene ID" value="Rmet_0687"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0687"/>
    <dbReference type="eggNOG" id="ENOG5030XPD">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1823761_0_0_4"/>
    <dbReference type="OMA" id="GERTCHG"/>
    <dbReference type="OrthoDB" id="1711283at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193753" evidence="1">
        <location>
            <begin position="35"/>
            <end position="138"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF07573.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="138" mass="14499" checksum="996335ABF33F6AB5" modified="2006-05-30" version="1">MTHAQAFLSLRRLAVPGATLAAALVLSACASSQASRFDVDAFLRGSDTALPEVLGNPDFLKATRIPQTDCATMLQSPHNGTLEDLPASNTARGPAWLLHPADTPDKVWLVVGEAGGERSCHGPLPADAMKKLTERAKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LQM9</accession>
    <name>Q1LQM9_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Major facilitator superfamily MFS_1 transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Rmet_0661</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07547.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515518.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0661"/>
    <dbReference type="EnsemblBacteria" id="ABF07547">
        <property type="protein sequence ID" value="ABF07547"/>
        <property type="gene ID" value="Rmet_0661"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0661"/>
    <dbReference type="eggNOG" id="COG2814">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001265_62_0_4"/>
    <dbReference type="OMA" id="WKISSFS"/>
    <dbReference type="OrthoDB" id="1345431at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="SSF103473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="24"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="63"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="96"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="122"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="149"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="181"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="230"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="265"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="296"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="324"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="356"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="391"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="domain" description="MFS" evidence="6">
        <location>
            <begin position="26"/>
            <end position="417"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABF07547.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="418" mass="45029" checksum="2D802F75820FCC90" modified="2006-05-30" version="1">MTASTTHPATDMASQPNHASGARWRVLAFFSLGFLVSYVFRGVNLGFAPYLTRELGLTAGDLGLLTSFYFLGFACAQLPAGILLDRFGPRRTEAVMLMLAVAGSLVFAWAPGMVGLAAGRAMIGVGVSVCLGAAIQALSMWFPLSRMPLLNGLVMAIGGLGAVVVGAPLSWLLSWTDWRTISAGMAVISFGMAALLWFGVPDVKRQGKESFAEQLRGTRQILSSERFWRVVPLTLLNQGIFLAVQTLWVTAFMRDVQDLGPGESARLVSVIGFAMMAGCIGAGWAARHLERRGISLYALAGTGMIGFIVIQLLLMARLPFMPLGLLWALYGVFGSTGILTYALLARRFPDALIGRATTAMTLTVFLATFVFQVGIGFVLDFFPLSGDHYPVSAHLMVWAGLVVIQVLAAIWYLMEKRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LQV6</accession>
    <name>Q1LQV6_CUPMC</name>
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        <submittedName>
            <fullName evidence="8">Putative CBS:Cl-channel, voltage gated</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ordered locus">Rmet_0584</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07470.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0584"/>
    <dbReference type="EnsemblBacteria" id="ABF07470">
        <property type="protein sequence ID" value="ABF07470"/>
        <property type="gene ID" value="Rmet_0584"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0584"/>
    <dbReference type="eggNOG" id="COG0038">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="eggNOG" id="COG0517">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015263_5_0_4"/>
    <dbReference type="OMA" id="LFEWKPR"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005247">
        <property type="term" value="F:voltage-gated chloride channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.580.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014743">
        <property type="entry name" value="Cl-channel_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001807">
        <property type="entry name" value="Cl-channel_volt-gated"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00571">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00654">
        <property type="entry name" value="Voltage_CLC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00762">
        <property type="entry name" value="CLCHANNEL"/>
    </dbReference>
    <dbReference type="SMART" id="SM00116">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="SSF54631"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81340">
        <property type="entry name" value="SSF81340"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51371">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0129">CBS domain</keyword>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="59"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="108"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="206"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="242"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="275"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="312"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="350"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="378"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="404"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="437"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="7">
        <location>
            <begin position="490"/>
            <end position="548"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="7">
        <location>
            <begin position="558"/>
            <end position="619"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ABF07470.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="637" mass="66822" checksum="CEF22427235F36FB" modified="2006-05-30" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="59" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LRD3</accession>
    <name>Q1LRD3_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_0407</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07293.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515283.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0407"/>
    <dbReference type="EnsemblBacteria" id="ABF07293">
        <property type="protein sequence ID" value="ABF07293"/>
        <property type="gene ID" value="Rmet_0407"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0407"/>
    <dbReference type="eggNOG" id="ENOG502ZBX5">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_067037_0_0_4"/>
    <dbReference type="OMA" id="DNLHDFF"/>
    <dbReference type="OrthoDB" id="1838579at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021390">
        <property type="entry name" value="DUF3025"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11227">
        <property type="entry name" value="DUF3025"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF07293.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="262" mass="28354" checksum="C0A7410AB5B44E3C" modified="2006-05-30" version="1">MLSTIDWTRPWFLPFAASASCLDGSDLREAFNAAALGSLNAQGLPLRFVPQDSLPEGVAYESHIFATGQVPTRDNLHDTFNALIWLHFPETKRLLNRLQAEAIERDGVQAVRGGLRDAATLFDENAVILVSEDATIANALRGFAWRKLFVSRRDAWRDTCAVVPFGHALLEKLVQPYKSVTAHAWWIPASPTTPIARLDGMLAASLAEAAEQGRLKGGRSFAPLPVMGIPGWCGESAAPGFYDDASVFRPGRRGAAANAEAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LRF3</accession>
    <name>Q1LRF3_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Acetyl-CoA C-acyltransferase</fullName>
            <ecNumber evidence="7">2.3.1.16</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">fadA</name>
        <name evidence="7" type="ordered locus">Rmet_0387</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the thiolase-like superfamily. Thiolase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.16" evidence="7"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07273.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0387"/>
    <dbReference type="EnsemblBacteria" id="ABF07273">
        <property type="protein sequence ID" value="ABF07273"/>
        <property type="gene ID" value="Rmet_0387"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0387"/>
    <dbReference type="eggNOG" id="COG0183">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031026_1_1_4"/>
    <dbReference type="OMA" id="DYYWGMG"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003988">
        <property type="term" value="F:acetyl-CoA C-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd00751">
        <property type="entry name" value="thiolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.47.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002155">
        <property type="entry name" value="Thiolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016039">
        <property type="entry name" value="Thiolase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020615">
        <property type="entry name" value="Thiolase_acyl_enz_int_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020610">
        <property type="entry name" value="Thiolase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020617">
        <property type="entry name" value="Thiolase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020613">
        <property type="entry name" value="Thiolase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020616">
        <property type="entry name" value="Thiolase_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02803">
        <property type="entry name" value="Thiolase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00108">
        <property type="entry name" value="Thiolase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000429">
        <property type="entry name" value="Ac-CoA_Ac_transf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53901">
        <property type="entry name" value="SSF53901"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01930">
        <property type="entry name" value="AcCoA-C-Actrans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00098">
        <property type="entry name" value="THIOLASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00737">
        <property type="entry name" value="THIOLASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00099">
        <property type="entry name" value="THIOLASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4 7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Thiolase_N" evidence="5">
        <location>
            <begin position="8"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="domain" description="Thiolase_C" evidence="6">
        <location>
            <begin position="277"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="active site" description="Acyl-thioester intermediate" evidence="3">
        <location>
            <position position="94"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="3">
        <location>
            <position position="354"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="3">
        <location>
            <position position="384"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR000429-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF02803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABF07273.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="398" mass="41626" checksum="91A76D8D39BF49AC" modified="2006-05-30" version="1">MKQLQDAYIVAATRSPIGKAPKGAFKNTRPDDLLATILQAAVAQVPSLDPALIEDAIVGCAIPEAQQGLNVARIGALLAGLPNTVGGITVNRFCASGLSAVAMAADRIRVGESDVMIAAGVESMSMVPMMGNSPSMSPSIFERDENIGIAYGMGLTAEKVATQWKVSREDQDAFSLASHQKAIAAQQAGEFKDEITPIEVVERFPDLASGQINVKTRTISLDEGPRPDTSVEGLGKLRAVFANKGSVTAGNSSQTSDGAGALILVSEKILKQFNLVPRARFVSFAVRGVPPEIMGIGPKAAIPAALKAAGLTQDQIDWIELNEAFAAQSLAVMRDLELDPAKVNRMGGAIALGHPLGATGAIRSATVVHALRRHNMKYGMVTMCVGTGMGAAGIFERV</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LRF7</accession>
    <name>Q1LRF7_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Cardiolipin synthase B</fullName>
            <shortName evidence="1">CL synthase</shortName>
            <ecNumber evidence="1">2.7.8.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">clsB</name>
        <name evidence="4" type="ordered locus">Rmet_0383</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the phosphatidyl group transfer from one phosphatidylglycerol molecule to another to form cardiolipin (CL) (diphosphatidylglycerol) and glycerol.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>2 1,2-diacyl-sn-glycero-3-phospho-(1'-sn-glycerol) = a cardiolipin + glycerol</text>
            <dbReference type="Rhea" id="RHEA:31451"/>
            <dbReference type="ChEBI" id="CHEBI:17754"/>
            <dbReference type="ChEBI" id="CHEBI:62237"/>
            <dbReference type="ChEBI" id="CHEBI:64716"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the phospholipase D family. Cardiolipin synthase subfamily. ClsB sub-subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.8.-" evidence="1"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07269.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0383"/>
    <dbReference type="EnsemblBacteria" id="ABF07269">
        <property type="protein sequence ID" value="ABF07269"/>
        <property type="gene ID" value="Rmet_0383"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0383"/>
    <dbReference type="eggNOG" id="COG1502">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038053_0_0_4"/>
    <dbReference type="OMA" id="EYCQRPL"/>
    <dbReference type="OrthoDB" id="1881748at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008808">
        <property type="term" value="F:cardiolipin synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032049">
        <property type="term" value="P:cardiolipin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01917">
        <property type="entry name" value="Cardiolipin_synth_ClsB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030872">
        <property type="entry name" value="Cardiolipin_synth_ClsB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025202">
        <property type="entry name" value="PLD-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001736">
        <property type="entry name" value="PLipase_D/transphosphatidylase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13091">
        <property type="entry name" value="PLDc_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00155">
        <property type="entry name" value="PLDc"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50035">
        <property type="entry name" value="PLD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="1" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="1" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword evidence="1" id="KW-1208">Phospholipid metabolism</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PLD phosphodiesterase" evidence="3">
        <location>
            <begin position="169"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="domain" description="PLD phosphodiesterase" evidence="3">
        <location>
            <begin position="363"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="17"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="174"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="176"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="181"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="368"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="370"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="375"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF07269.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="461" mass="51152" checksum="F6D3C575B4EF1386" modified="2006-05-30" version="1">MCRSRPFADNRAMSRINDRVSPPTSSAPGTPESPSSALHQEVGDPAATTVANATAPLMRWVRRRGSPVQGNLVDLLSNGQEYFPALIEAIDAARVHVVLETYIYADDDVGALVTDALIRAAQRGVDVRLVVDGFGAGDLPATLASRLRDGGVRLEVFRPVRGFRLARRHLRRLHRKMAVIDDSVAFVGGINIIDDHNYGPLELPEVGPRYDFAVRVRGPLVAQIALASARLWFRMAQQRGLRETATAFTEVETLTTAARQARREHHAGGVAAALLLRDNLRNRRTIEREYLYALGAARHDVIIANAYFLPGTKMRRALLACRERGVRVRLLLQGMVEYRLQHFATHALYARLLEAGIEIYEYEASFLHAKVAVIDNSWATVGSSNLDPFSLLLAREANVAVYDPSFAGVLRARLERAMAQRSARVVPEVHARLPRLRRFANWAAYYVLRLGVVIAGVTGRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LRK9</accession>
    <name>Q1LRK9_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Protein-tyrosine-phosphatase</fullName>
            <ecNumber evidence="2">3.1.3.48</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">arsC2</name>
        <name evidence="5" type="ordered locus">Rmet_0331</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>H2O + O-phospho-L-tyrosyl-[protein] = L-tyrosyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:10684"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:10137"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:82620"/>
            <dbReference type="EC" id="3.1.3.48"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="3.1.3.48" evidence="2"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07217.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515212.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0331"/>
    <dbReference type="EnsemblBacteria" id="ABF07217">
        <property type="protein sequence ID" value="ABF07217"/>
        <property type="gene ID" value="Rmet_0331"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0331"/>
    <dbReference type="eggNOG" id="COG0394">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_071415_3_0_4"/>
    <dbReference type="OMA" id="KPITAHW"/>
    <dbReference type="OrthoDB" id="1800563at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004725">
        <property type="term" value="F:protein tyrosine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023485">
        <property type="entry name" value="Ptyr_pPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036196">
        <property type="entry name" value="Ptyr_pPase_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01451">
        <property type="entry name" value="LMWPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00226">
        <property type="entry name" value="LMWPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52788">
        <property type="entry name" value="SSF52788"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="LMWPc" evidence="4">
        <location>
            <begin position="6"/>
            <end position="144"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABF07217.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="170" mass="18689" checksum="7D96CF4AA9C0D2A2" modified="2006-05-30" version="1">MTDKTFNVLVLCTGNSARSVMAEALFNVLGKGRFHAYSAGSHPSGTVNPFAIERCQAIGYDTTGLRSKSWDEFALPDAPRMDFVITVCDQAAGEVCPIWPGTPITAHWGFLDPAAIQGSDDEKRKIFAQVFRQILNRVSQFVNLPLHVLDRNAIQKEMHAIGEQPVESAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LRU8</accession>
    <name>Q1LRU8_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Glutamate-cysteine ligase</fullName>
            <ecNumber evidence="1">6.3.2.2</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">gshA</name>
        <name evidence="1" type="ordered locus">Rmet_0242</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EC" id="6.3.2.2" evidence="1"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07128.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515140.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0242"/>
    <dbReference type="EnsemblBacteria" id="ABF07128">
        <property type="protein sequence ID" value="ABF07128"/>
        <property type="gene ID" value="Rmet_0242"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0242"/>
    <dbReference type="eggNOG" id="COG0342">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_635836_0_0_4"/>
    <dbReference type="OMA" id="GMHFVPL"/>
    <dbReference type="OrthoDB" id="481578at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004357">
        <property type="term" value="F:glutamate-cysteine ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11280">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011718">
        <property type="entry name" value="GshA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042520">
        <property type="entry name" value="GshA_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08886">
        <property type="entry name" value="GshA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02049">
        <property type="entry name" value="gshA_ferroox"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0436">Ligase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF07128.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="431" mass="47956" checksum="6CE9DAA5ECD3F173" modified="2006-05-30" version="1">MVPHLITALNGPLLELEKKILDATPSIERWFRLEWQEHTPPFYCSVDLRNAGFKLAPVDTNLFPGGFNNLAPEMLPLAVQAAMAAIEKICPDAKNLLLIPERHTRNMFYLQNVARLSLIMRQAGLNVRLGSLSEEITEPTPIELPDGQTLVVEPLARLGAKGRRLGLKDFDPCSILLNNDLSAGIPPILENINEQYLLPPLHAGWSTRRKSSHFAAYDEVAKKFAKLIDIDPWMVNPYFARQTGVNFHERIGEEELADAVEGVLKKIAKKYREYGIKETPYVVVKADAGTYGMGIMTVRDPSEVKGLNRKERNKMSVVKEGLEVSDVIIQEGVHTFEKVNDAVAEPVVYMIDRYVVGGFYRVHTGRGIDENLNAPGMHFVPLAFAPNGMPDSHAKPGAAVPNRFYMYGVVARLALLAASLELEKTDPNPIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="115" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LRZ0</accession>
    <name>Q1LRZ0_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">3-isopropylmalate dehydratase large subunit</fullName>
            <ecNumber evidence="12">4.2.1.33</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Alpha-IPM isomerase</fullName>
            <shortName evidence="12">IPMI</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">Isopropylmalate isomerase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12 14" type="primary">leuC</name>
        <name evidence="14" type="ordered locus">Rmet_0200</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2 12">Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 12">
            <text>(2R,3S)-3-isopropylmalate = (2S)-2-isopropylmalate</text>
            <dbReference type="Rhea" id="RHEA:32287"/>
            <dbReference type="ChEBI" id="CHEBI:1178"/>
            <dbReference type="ChEBI" id="CHEBI:35121"/>
            <dbReference type="EC" id="4.2.1.33"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="12">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="12">Binds 1 [4Fe-4S] cluster per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3 12">Amino-acid biosynthesis; L-leucine biosynthesis; L-leucine from 3-methyl-2-oxobutanoate: step 2/4.</text>
    </comment>
    <comment type="subunit">
        <text evidence="12">Heterodimer of LeuC and LeuD.</text>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the aconitase/IPM isomerase family. LeuC type 1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.33" evidence="12"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07086.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0200"/>
    <dbReference type="EnsemblBacteria" id="ABF07086">
        <property type="protein sequence ID" value="ABF07086"/>
        <property type="gene ID" value="Rmet_0200"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0200"/>
    <dbReference type="eggNOG" id="COG0065">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006714_3_4_4"/>
    <dbReference type="OMA" id="GCGPCMG"/>
    <dbReference type="UniPathway" id="UPA00048">
        <property type="reaction ID" value="UER00071"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003861">
        <property type="term" value="F:3-isopropylmalate dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016853">
        <property type="term" value="F:isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009098">
        <property type="term" value="P:leucine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01583">
        <property type="entry name" value="IPMI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.499.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01026">
        <property type="entry name" value="LeuC_type1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004430">
        <property type="entry name" value="3-IsopropMal_deHydase_lsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015931">
        <property type="entry name" value="Acnase/IPM_dHydase_lsu_aba_1/3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001030">
        <property type="entry name" value="Acoase/IPM_deHydtase_lsu_aba"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018136">
        <property type="entry name" value="Aconitase_4Fe-4S_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036008">
        <property type="entry name" value="Aconitase_4Fe-4S_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033941">
        <property type="entry name" value="IPMI_cat"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00330">
        <property type="entry name" value="Aconitase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00415">
        <property type="entry name" value="ACONITASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53732">
        <property type="entry name" value="SSF53732"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00170">
        <property type="entry name" value="leuC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00450">
        <property type="entry name" value="ACONITASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01244">
        <property type="entry name" value="ACONITASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 12" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="6 12" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="11 12" id="KW-0100">Branched-chain amino acid biosynthesis</keyword>
    <keyword evidence="8 12" id="KW-0408">Iron</keyword>
    <keyword evidence="9 12" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="14" id="KW-0413">Isomerase</keyword>
    <keyword evidence="4 12" id="KW-0432">Leucine biosynthesis</keyword>
    <keyword evidence="10 12 14" id="KW-0456">Lyase</keyword>
    <keyword evidence="7 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aconitase" evidence="13">
        <location>
            <begin position="30"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="370"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="430"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="433"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ABF07086.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="493" mass="52423" checksum="542D14FBE9AEBD74" modified="2006-05-30" version="1">MDSAIRLRAFIHSNCIHPANAMTGRTLYQKLVDSHTVVTLDADNVLLYADLHIMNEYTSPQAFAGLAEQGRKVAAPGQNLAVVDHIIPTHAESPRVIQDPASALQARNLKRNCDLHGIPLFDTNDPLQGIEHVIAPEHGMIRPGMVVLCGDSHTTTYGAFGALGFGIGTSEVEHVLATQTLVYRLARDMRIRVDGPLPSGTTSKDLILHIIARISAQGARGYAVEFTGSTIRALSMEARMTLCNMTVEAGARGALIAPDATSIDYVLRRAPDIDADMAETARRAWAGLRSDADAGFDLELAFDAAAVEPFVTWGTSPDQAIPVSATVPQPADAGDSNARVSAERALEYIGVTPGVSLQGLPVQRVFIGSCTNGRIEDLRQVAAVVRGRRVAPGVRAMVVPGSGAVRDQAEAEGIAALLIDAGFEWRKPGCSMCLAMNDDVLAPGERCASTTNRNFEGRQGRAGRTHLMSPAMAAAAAVTGCITDVRELEIQHG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2008-01-15" modified="2022-08-03" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LS59</accession>
    <name>HSLV_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">ATP-dependent protease subunit HslV</fullName>
            <ecNumber evidence="1">3.4.25.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">hslV</name>
        <name type="ordered locus">Rmet_0131</name>
    </gene>
    <organism>
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Protease subunit of a proteasome-like degradation complex believed to be a general protein degrading machinery.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP-dependent cleavage of peptide bonds with broad specificity.</text>
            <dbReference type="EC" id="3.4.25.2"/>
        </reaction>
    </comment>
    <comment type="activity regulation">
        <text evidence="1">Allosterically activated by HslU binding.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">A double ring-shaped homohexamer of HslV is capped on each side by a ring-shaped HslU homohexamer. The assembly of the HslU/HslV complex is dependent on binding of ATP.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase T1B family. HslV subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.4.25.2" evidence="1"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF07017.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008642837.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q1LS59"/>
    <dbReference type="SMR" id="Q1LS59"/>
    <dbReference type="STRING" id="266264.Rmet_0131"/>
    <dbReference type="MEROPS" id="T01.006"/>
    <dbReference type="EnsemblBacteria" id="ABF07017">
        <property type="protein sequence ID" value="ABF07017"/>
        <property type="gene ID" value="Rmet_0131"/>
    </dbReference>
    <dbReference type="GeneID" id="60822853"/>
    <dbReference type="KEGG" id="rme:Rmet_0131"/>
    <dbReference type="eggNOG" id="COG5405">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_093872_1_0_4"/>
    <dbReference type="OMA" id="IMKGNAR"/>
    <dbReference type="OrthoDB" id="1129370at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009376">
        <property type="term" value="C:HslUV protease complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005839">
        <property type="term" value="C:proteasome core complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004298">
        <property type="term" value="F:threonine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051603">
        <property type="term" value="P:proteolysis involved in protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01913">
        <property type="entry name" value="protease_HslV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00248">
        <property type="entry name" value="HslV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022281">
        <property type="entry name" value="ATP-dep_Prtase_HsIV_su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001353">
        <property type="entry name" value="Proteasome_sua/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023333">
        <property type="entry name" value="Proteasome_suB-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32194">
        <property type="entry name" value="PTHR32194"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00227">
        <property type="entry name" value="Proteasome"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF039093">
        <property type="entry name" value="HslV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="SSF56235"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03692">
        <property type="entry name" value="ATP_dep_HslV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51476">
        <property type="entry name" value="PROTEASOME_BETA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0021">Allosteric enzyme</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0645">Protease</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0915">Sodium</keyword>
    <keyword id="KW-0888">Threonine protease</keyword>
    <feature type="chain" id="PRO_1000012655" description="ATP-dependent protease subunit HslV">
        <location>
            <begin position="1"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="7"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="162"/>
        </location>
        <ligand>
            <name>Na(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="165"/>
        </location>
        <ligand>
            <name>Na(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="168"/>
        </location>
        <ligand>
            <name>Na(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00248"/>
        </source>
    </evidence>
    <sequence length="178" mass="19031" checksum="4F0F3CA445A6C8B5" modified="2006-05-30" version="1">MEQYHGTTIVSVRRGNQVALGGDGQVTLGNIVMKGTARKVRRIYNGKVLVGFAGSTADAFSLLDRFEAKLEKYQGNLTRAAVDLAKDWRSDRALRRLEAMLITADKDTTLVITGNGDVLDPEGGIAAIGSGGAYAQSAAKALVENTELPPKDVVEKALTIAGELCIYTNTNFVIETLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LS85</accession>
    <name>Q1LS85_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Carbonic anhydrase</fullName>
            <ecNumber evidence="3">4.2.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">can</name>
        <name evidence="6" type="ordered locus">Rmet_0105</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>H(+) + hydrogencarbonate = CO2 + H2O</text>
            <dbReference type="Rhea" id="RHEA:10748"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <dbReference type="EC" id="4.2.1.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the beta-class carbonic anhydrase family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.1" evidence="3"/>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF06991.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515025.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0105"/>
    <dbReference type="EnsemblBacteria" id="ABF06991">
        <property type="protein sequence ID" value="ABF06991"/>
        <property type="gene ID" value="Rmet_0105"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0105"/>
    <dbReference type="eggNOG" id="COG0288">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_053879_3_0_4"/>
    <dbReference type="OMA" id="PANEIIG"/>
    <dbReference type="OrthoDB" id="1841447at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004089">
        <property type="term" value="F:carbonate dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015976">
        <property type="term" value="P:carbon utilization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1050.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001765">
        <property type="entry name" value="Carbonic_anhydrase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015892">
        <property type="entry name" value="Carbonic_anhydrase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036874">
        <property type="entry name" value="Carbonic_anhydrase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11002">
        <property type="entry name" value="PTHR11002"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00484">
        <property type="entry name" value="Pro_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00947">
        <property type="entry name" value="Pro_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53056">
        <property type="entry name" value="SSF53056"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00704">
        <property type="entry name" value="PROK_CO2_ANHYDRASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0456">Lyase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012925"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABF06991.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="223" mass="24625" checksum="6484BC5E7350602B" modified="2006-05-30" version="1">MSDAIAQLFRNNREWVDRVNAEDPTFFTRLANQQAPEYLWIGCSDSRVPANQILGLAPGEVFVHRNIANVISHSDLNALSVLQFAVDVLKVRHVTVVGHYGCGGVKVALKRERVGLADNWLRHVQDVADKHAGYLGTVLREDDAHTRLCELNVIEQVNNVCQTTVLQDAWARGQAVTVHGWVYGVSDGLLRDLGMAASNNDELHAQIAAALRQYGDPPQASIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="68" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LSA4</accession>
    <name>Q1LSA4_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative sulfurtransferase (Rhodanese-like protein)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_0086</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF06972.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_008644527.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0086"/>
    <dbReference type="EnsemblBacteria" id="ABF06972">
        <property type="protein sequence ID" value="ABF06972"/>
        <property type="gene ID" value="Rmet_0086"/>
    </dbReference>
    <dbReference type="GeneID" id="60822808"/>
    <dbReference type="KEGG" id="rme:Rmet_0086"/>
    <dbReference type="eggNOG" id="COG0607">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_089574_10_1_4"/>
    <dbReference type="OMA" id="FIDCRSE"/>
    <dbReference type="OrthoDB" id="2056793at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.250.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001763">
        <property type="entry name" value="Rhodanese-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036873">
        <property type="entry name" value="Rhodanese-like_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00581">
        <property type="entry name" value="Rhodanese"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00450">
        <property type="entry name" value="RHOD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52821">
        <property type="entry name" value="SSF52821"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50206">
        <property type="entry name" value="RHODANESE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Rhodanese" evidence="1">
        <location>
            <begin position="15"/>
            <end position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF06972.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="130" mass="14454" checksum="450BAE534A8173A1" modified="2006-05-30" version="1">MQTLSPNDARTLLEGEPDALFIDCRSEMEYLFVGHPRGAHNVPWNDGPDWEVNPHFVQAVKKLAGQASARPVVLICRSGNRSSAAARALEGAGFATIYCVLHGFEGDLDAQRHRNTVNGWRHDGLPWEQY</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LSA6</accession>
    <name>Q1LSA6_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Predicted hydrolase or acyltransferase (Alpha/beta hydrolase superfamily)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_0084</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF06970.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011515007.1">
        <property type="nucleotide sequence ID" value="NC_007973.1"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_0084"/>
    <dbReference type="ESTHER" id="ralme-q1lsa6">
        <property type="family name" value="Carboxymethylbutenolide_lactonase"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF06970">
        <property type="protein sequence ID" value="ABF06970"/>
        <property type="gene ID" value="Rmet_0084"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0084"/>
    <dbReference type="eggNOG" id="COG2021">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020336_50_3_4"/>
    <dbReference type="OMA" id="WGWQHAA"/>
    <dbReference type="OrthoDB" id="1196738at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047570">
        <property type="term" value="F:3-oxoadipate enol-lactonase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016746">
        <property type="term" value="F:acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042952">
        <property type="term" value="P:beta-ketoadipate pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026968">
        <property type="entry name" value="PcaD/CatD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00561">
        <property type="entry name" value="Abhydrolase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00111">
        <property type="entry name" value="ABHYDROLASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="SSF53474"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02427">
        <property type="entry name" value="protocat_pcaD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="AB hydrolase-1" evidence="1">
        <location>
            <begin position="32"/>
            <end position="258"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF06970.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="282" mass="30754" checksum="63F1F75D169E2B29" modified="2006-05-30" version="1">MSDTSASTASQTNRLRVGDVHLQVRVDGTEGPWVILAHALGANLTLWDDTARHLAARYRVLRFDMRGHGGSDAPVGAYTMTRLADDVVALMDELDIAQAHFCGVSVGGMVAQTLGVRHPERLLSLTLVDTIHHTPLEARAMWADRIGQVEAHGMGGTVESTLNRWLTAPFRERHPEIVERIRKMLLETPVRGYVGVAQAIEAFDLARAISRIHCPTLVVVGDKDEGSPVSIAEAIAREIHGSRLEVLPDAAHLSFIEQPERFHAMFDAFLGHAACGGQCDIP</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LSG7</accession>
    <name>Q1LSG7_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Major facilitator superfamily MFS_1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">fsr</name>
        <name evidence="7" type="ordered locus">Rmet_0023</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
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        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
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    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="8">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
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        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
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        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="EnsemblBacteria" id="ABF06909">
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        <property type="gene ID" value="Rmet_0023"/>
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        <property type="taxonomic scope" value="Bacteria"/>
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        <property type="component" value="Chromosome"/>
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        <property type="entry name" value="MFS_dom"/>
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    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="SSF103473"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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            <end position="50"/>
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            <begin position="62"/>
            <end position="83"/>
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        <location>
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        </location>
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        </location>
    </feature>
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            <end position="283"/>
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    </feature>
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            <end position="339"/>
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        <location>
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            <end position="399"/>
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    </feature>
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        <location>
            <begin position="26"/>
            <end position="405"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="ABF06909.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="409" mass="43827" checksum="CCC8D12C8E127BD5" modified="2006-05-30" version="1">MRPRTMSTTINPAHPADREEGTRFRVLYAISFAHFLNDMIQSLILAIYPMLKGGFNLSFTQIGLLTLTYQVTASLLQPVVGLYTDKHPKPHSLAIGMGFTLCGLLLLSVAPTYGVLLVAAALVGTGSSIFHPESSRVARLASGGQHGLAQSIFQVGGNGGSAMGPLLAAWIVHSQGSVAWFSLAALLAITVLWQIGGWYGRHLAERAAKKKGAGVVVNKLPKSTVVRAIVVLLLLIFSKYFYMASLNTYYTFYLMEKFGLARQTSQIYLFLFLFAVAAGTIMGGPIGDRIGRKRVIWASILGVAPFTLMLPHANLFWTAVLSVVIGFILASAFSAILVFAQELIPGKVGMVSGLFFGFAFGMGGIGAAVLGRLADAHGIETVYQLCAFLPLLGLLAVFLPDLRERAKAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2005-04-26" modified="2022-01-19" version="111" xmlns="http://uniprot.org/uniprot">
    <accession>Q58AF3</accession>
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        <submittedName>
            <fullName evidence="5">SilB,Silver efflux protein, MFP component of the three components proton antiporter metal efflux system</fullName>
        </submittedName>
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    <gene>
        <name evidence="5" type="primary">silB</name>
        <name evidence="5" type="ordered locus">Rmet_6135</name>
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        <lineage>
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    <geneLocation type="plasmid" evidence="5 6">
        <name>pMOL30</name>
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    <reference evidence="6" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="6">Plasmid pMOL30</plasmid>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="journal article" date="2011" name="Biochemistry" volume="50" first="2194" last="2204">
            <title>Structural and metal binding characterization of the C-terminal metallochaperone domain of membrane fusion protein SilB from Cupriavidus metallidurans CH34.</title>
            <authorList>
                <person name="Bersch B."/>
                <person name="Derfoufi K.M."/>
                <person name="De Angelis F."/>
                <person name="Auquier V."/>
                <person name="Ekende E.N."/>
                <person name="Mergeay M."/>
                <person name="Ruysschaert J.M."/>
                <person name="Vandenbussche G."/>
            </authorList>
            <dbReference type="PubMed" id="21299248"/>
            <dbReference type="DOI" id="10.1021/bi200005k"/>
        </citation>
        <scope>STRUCTURE BY NMR OF 440-521</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="journal article" date="2016" name="Biochemistry" volume="55" first="2883" last="2897">
            <title>Structural and Functional Investigation of the Ag(+)/Cu(+) Binding Domains of the Periplasmic Adaptor Protein SilB from Cupriavidus metallidurans CH34.</title>
            <authorList>
                <person name="Urbina P."/>
                <person name="Bersch B."/>
                <person name="De Angelis F."/>
                <person name="Derfoufi K.M."/>
                <person name="Prevost M."/>
                <person name="Goormaghtigh E."/>
                <person name="Vandenbussche G."/>
            </authorList>
            <dbReference type="PubMed" id="27145046"/>
            <dbReference type="DOI" id="10.1021/ACS.BIOCHEM.6B00022"/>
        </citation>
        <scope>STRUCTURE BY NMR OF 36-123 AND 344-426</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family.</text>
    </comment>
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        <property type="protein sequence ID" value="ABF12994.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_006466.1"/>
    </dbReference>
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        <property type="chains" value="A=440-521"/>
    </dbReference>
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        <property type="method" value="NMR"/>
        <property type="chains" value="A=36-123, A=344-426"/>
    </dbReference>
    <dbReference type="PDBsum" id="2L55"/>
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    <dbReference type="BMRB" id="Q58AF3"/>
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    <dbReference type="EnsemblBacteria" id="ABF12994">
        <property type="protein sequence ID" value="ABF12994"/>
        <property type="gene ID" value="Rmet_6135"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6135"/>
    <dbReference type="HOGENOM" id="CLU_018816_13_1_4"/>
    <dbReference type="OMA" id="WESHVEY"/>
    <dbReference type="OrthoDB" id="949250at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid pMOL30"/>
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        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="project" value="InterPro"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="CusF_Ec"/>
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    <dbReference type="InterPro" id="IPR042230">
        <property type="entry name" value="CusF_sf"/>
    </dbReference>
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        <property type="entry name" value="HlyD_D23"/>
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    <dbReference type="InterPro" id="IPR006143">
        <property type="entry name" value="RND_pump_MFP"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11604">
        <property type="entry name" value="CusF_Ec"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16576">
        <property type="entry name" value="HlyD_D23"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="RND_mfp"/>
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    </dbReference>
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    <keyword evidence="3" id="KW-0472">Membrane</keyword>
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    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
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        <location>
            <begin position="6"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="domain" description="HlyD_D23" evidence="4">
        <location>
            <begin position="123"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="421"/>
            <end position="445"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
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    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF16576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABF12994.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="7">
        <source>
            <dbReference type="PDB" id="2L55"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="8">
        <source>
            <dbReference type="PDB" id="5A4G"/>
        </source>
    </evidence>
    <sequence length="521" mass="54896" checksum="FEEEB11B2C6B7E19" modified="2005-04-26" version="1"> </sequence>
</entry>
<entry dataset="TrEMBL" created="2010-03-23" modified="2020-10-07" version="28" xmlns="http://uniprot.org/uniprot">
    <accession>D3DXM2</accession>
    <name>D3DXM2_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_6425</name>
    </gene>
    <organism evidence="1 2">
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        <name type="synonym">Ralstonia metallidurans</name>
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    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="EnsemblBacteria" id="ADC45042">
        <property type="protein sequence ID" value="ADC45042"/>
        <property type="gene ID" value="Rmet_6425"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6425"/>
    <dbReference type="eggNOG" id="ENOG50317IT">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2900983_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADC45042.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="62" mass="6782" checksum="924E602ED731D890" modified="2010-03-23" version="1">MGFMFGGAGGNRTRVRKPSTESSTYLVLSFDLTGASRTNTLYDGESLGFRPRARDTPEAYLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-03-23" modified="2020-10-07" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>D3DXW7</accession>
    <name>D3DXW7_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_6530</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
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        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ADC45137.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_6530"/>
    <dbReference type="EnsemblBacteria" id="ADC45137">
        <property type="protein sequence ID" value="ADC45137"/>
        <property type="gene ID" value="Rmet_6530"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6530"/>
    <dbReference type="HOGENOM" id="CLU_3188145_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADC45137.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="41" mass="4443" checksum="351BBE86A299E620" modified="2010-03-23" version="1">MRLAKGLGFLALAALLTLGFLAYLRPAFMVDLTNMVLAWCG</sequence>
</entry>
<entry dataset="TrEMBL" created="2010-03-23" modified="2020-10-07" version="34" xmlns="http://uniprot.org/uniprot">
    <accession>D3DYH1</accession>
    <name>D3DYH1_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_6762</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ADC45341.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_6762"/>
    <dbReference type="EnsemblBacteria" id="ADC45341">
        <property type="protein sequence ID" value="ADC45341"/>
        <property type="gene ID" value="Rmet_6762"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6762"/>
    <dbReference type="HOGENOM" id="CLU_2938426_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035919">
        <property type="entry name" value="EAL_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF141868">
        <property type="entry name" value="SSF141868"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ADC45341.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="60" mass="6559" checksum="A3D3D0A7A6DAF32B" modified="2010-03-23" version="1">MDSDHRSGRTFLWHPTTRCALVETIAQFNAMRELGCAFGQAQLGAPALPAAEFLALALQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-10-07" version="50" xmlns="http://uniprot.org/uniprot">
    <accession>Q1L9I6</accession>
    <name>Q1L9I6_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_6331</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3 4">
        <name>pMOL28</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid pMOL28</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000355">
        <property type="protein sequence ID" value="ABF13190.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF13190">
        <property type="protein sequence ID" value="ABF13190"/>
        <property type="gene ID" value="Rmet_6331"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6331"/>
    <dbReference type="HOGENOM" id="CLU_729317_0_0_4"/>
    <dbReference type="OrthoDB" id="2120135at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid pMOL28"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="318"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="347"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF13190.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="379" mass="42940" checksum="A4A1C903C69B71CF" modified="2006-05-30" version="1">MAARAAIQPDLHGDDQARRNGHQPCAFTAHQDPRFFMTDLQVVEPQQLELGVSSGMLEASAALCALKITLDRHARRFVGQAYLNDWMGGPGTCAFWGDEFLEVVIPFLRWEDGHSRPFQAFVDPRFVLGASIKGKPEDIAAAEVPSRIKKYAETFGSIDNVHYIWYRALGILCAHEGKHRVAFMRAHDQPAIAAWVSEAMYPDADRITIVKPDEGEEWLAILDGRYVQVLRRPEVSSAMMRAYGVKEARWRELVDMPDKDLVIQAVRQRNFHNQPRSSAEEDRALDLDELARKERSDAELVSRTIHDIEPFRLDWKRYWFPVIACMVVGILLKLFDSPKTDEPALVLLGVACGLAASMSLLKFWGPRSVAGRSRAFWRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="101" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LA93</accession>
    <name>Q1LA93_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Transposase catalytic site ISRme3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">tnpB</name>
        <name evidence="2" type="ordered locus">Rmet_0031</name>
        <name evidence="3" type="ordered locus">Rmet_1612</name>
        <name evidence="4" type="ordered locus">Rmet_3348</name>
        <name evidence="5" type="ordered locus">Rmet_3771</name>
        <name evidence="6" type="ordered locus">Rmet_3942</name>
        <name evidence="7" type="ordered locus">Rmet_4659</name>
        <name evidence="8" type="ordered locus">Rmet_5452</name>
        <name evidence="9" type="ordered locus">Rmet_5679</name>
        <name evidence="10" type="ordered locus">Rmet_5973</name>
        <name evidence="11" type="ordered locus">Rmet_6074</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="5">
        <name>megaplasmid</name>
    </geneLocation>
    <geneLocation type="plasmid" evidence="12">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <geneLocation type="plasmid" evidence="11 12">
        <name>pMOL30</name>
    </geneLocation>
    <reference evidence="11" key="1">
        <citation type="submission" date="2006-04" db="EMBL/GenBank/DDBJ databases">
            <title>Complete sequence of the chromosome of Ralstonia metallidurans CH34.</title>
            <authorList>
                <consortium name="US DOE Joint Genome Institute"/>
                <person name="Copeland A."/>
                <person name="Lucas S."/>
                <person name="Lapidus A."/>
                <person name="Barry K."/>
                <person name="Detter J.C."/>
                <person name="Glavina del Rio T."/>
                <person name="Hammon N."/>
                <person name="Israni S."/>
                <person name="Dalin E."/>
                <person name="Tice H."/>
                <person name="Martinez M."/>
                <person name="Goltsman E."/>
                <person name="Pitluck S."/>
                <person name="Schmutz J."/>
                <person name="Larimer F."/>
                <person name="Land M."/>
                <person name="Hauser L."/>
                <person name="Kyrpides N."/>
                <person name="Kim E."/>
                <person name="Mergeay M."/>
                <person name="Benotmane M.A."/>
                <person name="Vallaeys T."/>
                <person name="Michaux A."/>
                <person name="Monchy S."/>
                <person name="Dunn J."/>
                <person name="McCorkle S."/>
                <person name="Taghavi S."/>
                <person name="van der Lelie D."/>
                <person name="Richardson P."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">CH34</strain>
            <plasmid evidence="5">megaplasmid</plasmid>
            <plasmid evidence="11">pMOL30</plasmid>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="12">Plasmid megaplasmid CH34</plasmid>
            <plasmid evidence="12">Plasmid pMOL30</plasmid>
        </source>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2010-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Benotmane R."/>
                <person name="Lapidus A."/>
                <person name="McCorkle S."/>
                <person name="Monchy S."/>
                <person name="Taghavi S."/>
                <person name="van der Lelie N."/>
                <person name="Dunn J."/>
                <person name="Leys N."/>
                <person name="Mergeay M."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">CH34</strain>
            <plasmid evidence="5">megaplasmid</plasmid>
            <plasmid evidence="11">pMOL30</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF06917.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF08495.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP000352">
        <property type="protein sequence ID" value="ABF10220.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10641.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF10810.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11521.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12311.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12538.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP000354">
        <property type="protein sequence ID" value="ABF12832.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="CP000354">
        <property type="protein sequence ID" value="ABF12933.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF06917">
        <property type="protein sequence ID" value="ABF06917"/>
        <property type="gene ID" value="Rmet_0031"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF08495">
        <property type="protein sequence ID" value="ABF08495"/>
        <property type="gene ID" value="Rmet_1612"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF10220">
        <property type="protein sequence ID" value="ABF10220"/>
        <property type="gene ID" value="Rmet_3348"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF10641">
        <property type="protein sequence ID" value="ABF10641"/>
        <property type="gene ID" value="Rmet_3771"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF10810">
        <property type="protein sequence ID" value="ABF10810"/>
        <property type="gene ID" value="Rmet_3942"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF11521">
        <property type="protein sequence ID" value="ABF11521"/>
        <property type="gene ID" value="Rmet_4659"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF12311">
        <property type="protein sequence ID" value="ABF12311"/>
        <property type="gene ID" value="Rmet_5452"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF12538">
        <property type="protein sequence ID" value="ABF12538"/>
        <property type="gene ID" value="Rmet_5679"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF12832">
        <property type="protein sequence ID" value="ABF12832"/>
        <property type="gene ID" value="Rmet_5973"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF12933">
        <property type="protein sequence ID" value="ABF12933"/>
        <property type="gene ID" value="Rmet_6074"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_0031"/>
    <dbReference type="KEGG" id="rme:Rmet_1612"/>
    <dbReference type="KEGG" id="rme:Rmet_3348"/>
    <dbReference type="KEGG" id="rme:Rmet_3771"/>
    <dbReference type="KEGG" id="rme:Rmet_3942"/>
    <dbReference type="KEGG" id="rme:Rmet_4659"/>
    <dbReference type="KEGG" id="rme:Rmet_5452"/>
    <dbReference type="KEGG" id="rme:Rmet_5679"/>
    <dbReference type="KEGG" id="rme:Rmet_5973"/>
    <dbReference type="KEGG" id="rme:Rmet_6074"/>
    <dbReference type="eggNOG" id="COG2801">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027402_4_2_4"/>
    <dbReference type="OMA" id="RIMREDN"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid pMOL30"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025948">
        <property type="entry name" value="HTH-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001584">
        <property type="entry name" value="Integrase_cat-core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13276">
        <property type="entry name" value="HTH_21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00665">
        <property type="entry name" value="rve"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13333">
        <property type="entry name" value="rve_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="SSF53098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50994">
        <property type="entry name" value="INTEGRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="11" id="KW-0614">Plasmid</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Integrase catalytic" evidence="1">
        <location>
            <begin position="127"/>
            <end position="288"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF06917.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF08495.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF10220.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ABF10641.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ABF10810.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABF11521.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ABF12311.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ABF12538.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ABF12832.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ABF12933.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="297" mass="34147" checksum="D110782ABDA863EF" modified="2006-05-30" version="1">MRYAFIERNRRYWPVSVLCELLGVSPSGYHQRKQRTVSTDRPDRGRLSDDALLAHIKAIHAGVKGEYGWPRMWKELLARGVRVGKERVRKLMALHGIRARHKRKYIATTNSNHDLPVAPNLLQRDFSPAAPNQVWTSDITYVATAEGWLYLVVIIDLFSRQVVGWSMQPHMKAELVTDALRMAWFRRRPEAGVIVHTDRGSQYCSHLFQDALKAYGMRSSMSRRGDCWDNAPTESLWGSLKVARLHGRQFATRRAAMDEVIDWLGFYNASRLHSTLGYVSPMTFEKNWSAAQQHRAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-10-07" version="43" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LAD0</accession>
    <name>Q1LAD0_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_6037</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3 4">
        <name>pMOL30</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid pMOL30</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000354">
        <property type="protein sequence ID" value="ABF12896.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF12896">
        <property type="protein sequence ID" value="ABF12896"/>
        <property type="gene ID" value="Rmet_6037"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6037"/>
    <dbReference type="HOGENOM" id="CLU_2024815_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid pMOL30"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="30"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="74"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="103"/>
            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF12896.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="122" mass="12940" checksum="7E08353EC691E568" modified="2006-05-30" version="1">MAPGAISSPPQSQETGTGVRMKRSDMIGALMLGVTLAAFVMACIKSGVGLAFWNLPALSEEARAGFGKTFLMDFIMLNVFFVAGPLTLYPLFGQSQFFAGRPQGESDGAIHPGTHLNGMVER</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-10-07" version="46" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LAD9</accession>
    <name>Q1LAD9_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_6028</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="1 2">
        <name>pMOL30</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid pMOL30</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000354">
        <property type="protein sequence ID" value="ABF12887.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="ABF12887">
        <property type="protein sequence ID" value="ABF12887"/>
        <property type="gene ID" value="Rmet_6028"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_6028"/>
    <dbReference type="HOGENOM" id="CLU_2024816_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid pMOL30"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF12887.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="122" mass="13405" checksum="94A5AC22FC49BF33" modified="2006-05-30" version="1">MATEIVAMSRRRWKCESLSDGSSHMRIEFCQQGTWVALEAARAWCRENGLSVGQSCATGPSGLLFGKVDWIAKWRNLAEDERDALHGTMSGDFREGPIVIVLKDEAVAAHMAVMKAKNPPTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LAT5</accession>
    <name>Q1LAT5_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Extra-cytoplasmic solute receptor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">bug</name>
        <name evidence="4" type="ordered locus">Rmet_5882</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="5">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="5">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Periplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the UPF0065 (bug) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12741.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5882"/>
    <dbReference type="EnsemblBacteria" id="ABF12741">
        <property type="protein sequence ID" value="ABF12741"/>
        <property type="gene ID" value="Rmet_5882"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5882"/>
    <dbReference type="eggNOG" id="COG3181">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045683_0_0_4"/>
    <dbReference type="OMA" id="HAANEHL"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042597">
        <property type="term" value="C:periplasmic space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005064">
        <property type="entry name" value="BUG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042100">
        <property type="entry name" value="Bug_dom1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42928">
        <property type="entry name" value="PTHR42928"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03401">
        <property type="entry name" value="TctC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017082">
        <property type="entry name" value="YflP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-0675">Receptor</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193248" evidence="3">
        <location>
            <begin position="32"/>
            <end position="328"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF12741.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="328" mass="34487" checksum="F4CF9A4101D61ADB" modified="2006-05-30" version="1">MSAFRRPQAPAALLAFATFTCLALPAGSAWAEFPDKPIRFVVPFAAGSATDQLARAVGQAVTQEAKVTVVVDNKPGANGFIAAGDVAKAAPDGYTVLITTNTTHAANEHLFKKLPYDPVKDYAPLTALGRGGQIMVVNPQVPAKTVGEFIALAKKEPGKLSFGSGSSSSRIAGELFQQMAHVELLHVPYKSNPLAITDLLGNQIQMMITDTATGLPQVKSGKLRALGVSGKARSPLAPDVPTIDEAGVKGYEMSYWFAAYAPAGTPPAVVGKLNQMLVKAARSETAATFYQSTGTDVFTSSPAELAKFQAQESAKWGRIIKAANIQPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LB96</accession>
    <name>Q1LB96_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Outer membrane protein (Porin)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ordered locus">Rmet_5721</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="10">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="10">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12580.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011520121.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5721"/>
    <dbReference type="EnsemblBacteria" id="ABF12580">
        <property type="protein sequence ID" value="ABF12580"/>
        <property type="gene ID" value="Rmet_5721"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5721"/>
    <dbReference type="eggNOG" id="COG3203">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038238_2_0_4"/>
    <dbReference type="OMA" id="GIEHINN"/>
    <dbReference type="OrthoDB" id="1064531at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009279">
        <property type="term" value="C:cell outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046930">
        <property type="term" value="C:pore complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015288">
        <property type="term" value="F:porin activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034220">
        <property type="term" value="P:ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00342">
        <property type="entry name" value="gram_neg_porins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.160.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033900">
        <property type="entry name" value="Gram_neg_porin_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023614">
        <property type="entry name" value="Porin_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001702">
        <property type="entry name" value="Porin_Gram-ve"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002299">
        <property type="entry name" value="Porin_Neis"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13609">
        <property type="entry name" value="Porin_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00182">
        <property type="entry name" value="ECOLNEIPORIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00184">
        <property type="entry name" value="NEISSPPORIN"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0406">Ion transport</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0614">Plasmid</keyword>
    <keyword evidence="5" id="KW-0626">Porin</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1134">Transmembrane beta strand</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193270" evidence="7">
        <location>
            <begin position="21"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="domain" description="Porin_4" evidence="8">
        <location>
            <begin position="8"/>
            <end position="317"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF13609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ABF12580.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="352" mass="37317" checksum="05016E6565576F71" modified="2006-05-30" version="1">MKIKMLTAAVVALASTGAFAQSSVTMYGVADVGIEYLTHAGGDNSTVRMSSGNLSGSRWGLRGTEDLGGGLKGIFTLESGINIDTGSGADSSRMFNRQAFVGLQKDGIGTLTLGRQQTLLYDFGLNYDPMAIATRYSIIGQDGAFASRADNAIKYVGTFGGVTASALYSFRYNGQEVPGQTSRGAEYSAGLNYATGPLSVGAVYDQVRLGLTAGVADTGAKIQRATIAGTYMFGPAKVFGGWRWANQTMADNHVRSNLWWTGLGYQVTPALSLTGAVYYQAFHQTSADPWTLVASADYALSKRTDLYLNMAYAINKKDSDRGIVSVVGVSNADQPINGQNQFGAVVGIRHKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-10-07" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LBA4</accession>
    <name>Q1LBA4_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">HPt domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_5713</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12572.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5713"/>
    <dbReference type="EnsemblBacteria" id="ABF12572">
        <property type="protein sequence ID" value="ABF12572"/>
        <property type="gene ID" value="Rmet_5713"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5713"/>
    <dbReference type="eggNOG" id="COG2198">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1234188_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00088">
        <property type="entry name" value="HPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.160">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036641">
        <property type="entry name" value="HPT_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008207">
        <property type="entry name" value="Sig_transdc_His_kin_Hpt_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01627">
        <property type="entry name" value="Hpt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47226">
        <property type="entry name" value="SSF47226"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50894">
        <property type="entry name" value="HPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HPt" evidence="2">
        <location>
            <begin position="129"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="modified residue" description="Phosphohistidine" evidence="1">
        <location>
            <position position="168"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF12572.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="224" mass="23344" checksum="95FEED2546278620" modified="2006-05-30" version="1">MSRVIISGEEWSGAERDELAASLTSLGLFTEEASAAASTDAADVVVVRWPLSVASQACLSVHSPPCLRLLMGDAGSLPSELPLSIDAMLPLHAGTVVIAETFAGLGFVPFSAADGERLWPALDALTGGDADIRMELVQSLVLTNTEDLVALSAACHALDWDQARAMAHRIKGTARMLDCQATVAVCQAIETASIRQDGDTVTALVPLLTQAIQRLLAALGAVPD</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-10-07" version="48" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LBB3</accession>
    <name>Q1LBB3_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_5704</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12563.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5704"/>
    <dbReference type="EnsemblBacteria" id="ABF12563">
        <property type="protein sequence ID" value="ABF12563"/>
        <property type="gene ID" value="Rmet_5704"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5704"/>
    <dbReference type="HOGENOM" id="CLU_169672_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF12563.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="106" mass="11967" checksum="B03DF9B462D48849" modified="2006-05-30" version="1">MVPCRQHQTLSPEAPMNSKIHLTGFHRELWELRSRGTGFCGLGGILPSSLFKDWATIKPLLPARDRYSQAKLALVFYAYLAVRDPAIVTRSHGGTGVHFTYRPWQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-09-29" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LBC4</accession>
    <name>Q1LBC4_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_5693</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12552.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011520094.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5693"/>
    <dbReference type="EnsemblBacteria" id="ABF12552">
        <property type="protein sequence ID" value="ABF12552"/>
        <property type="gene ID" value="Rmet_5693"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5693"/>
    <dbReference type="eggNOG" id="COG3391">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1309265_0_0_4"/>
    <dbReference type="OMA" id="WHGTWEG"/>
    <dbReference type="OrthoDB" id="966287at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002372">
        <property type="entry name" value="PQQ_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011044">
        <property type="entry name" value="Quino_amine_DH_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13360">
        <property type="entry name" value="PQQ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50969">
        <property type="entry name" value="SSF50969"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="1" id="KW-0564">Palmitate</keyword>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF12552.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="212" mass="22680" checksum="BD6D827D2C436591" modified="2006-05-30" version="1">MKRTNATILREFGPFPGVEQVNGVTYDGKQVWMAVGNNLVALDPATGKTLQTIDVAAHAGTAFDGRHIFQIGDDRIRKIDPVTGRVLATIPAPGGGGDSGLAWAEGTLWVGEYRERKIHQIDPETGAILRTITSDRFVTGVSWVDGTLWHGAGEGDQGALRRIDAATGEVLEQIDMPPGVMVSGLESDGKEQFFCGGGASSKLRVVRRPARE</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-10-07" version="51" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LBJ6</accession>
    <name>Q1LBJ6_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_5621</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12480.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5621"/>
    <dbReference type="EnsemblBacteria" id="ABF12480">
        <property type="protein sequence ID" value="ABF12480"/>
        <property type="gene ID" value="Rmet_5621"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5621"/>
    <dbReference type="HOGENOM" id="CLU_210411_0_0_4"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF12480.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="45" mass="4747" checksum="622D2AA3A19FAB06" modified="2006-05-30" version="1">MNKRFYLFCATTWAAAAVLILGQGSMATDALSGVIALAGFDLLRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LBM7</accession>
    <name>Q1LBM7_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="3">RND efflux system, outer membrane lipoprotein, NodT family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">Rmet_5590</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="4">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="4">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the outer membrane factor (OMF) (TC 1.B.17) family.</text>
    </comment>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12449.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519998.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5590"/>
    <dbReference type="EnsemblBacteria" id="ABF12449">
        <property type="protein sequence ID" value="ABF12449"/>
        <property type="gene ID" value="Rmet_5590"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5590"/>
    <dbReference type="eggNOG" id="COG1538">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012817_13_0_4"/>
    <dbReference type="OMA" id="WWHALGD"/>
    <dbReference type="OrthoDB" id="1619477at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015562">
        <property type="term" value="F:efflux transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003423">
        <property type="entry name" value="OMP_efflux"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010131">
        <property type="entry name" value="RND_efflux_OM_lipoprot_NodT"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02321">
        <property type="entry name" value="OEP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01845">
        <property type="entry name" value="outer_NodT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0564">Palmitate</keyword>
    <keyword evidence="3" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1134">Transmembrane beta strand</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5001442026" evidence="2">
        <location>
            <begin position="19"/>
            <end position="485"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU362097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ABF12449.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="485" mass="51816" checksum="E13326C68C877199" modified="2006-05-30" version="1">MSVSFRATLLTLAAAMFAAGCTVGPDYRGAPQVAGNAIDTGKFPRAGEGTDSQTPRVADWWHALGDTQLDGLIESALKQSPDIRVARARVRQARAGLAGNKANLFPTVAVDAATLRTRSPNLSALSGQGSSNAGRGPLSLYIAAFDASWEIDLFGGTRRSIEAASAEADAADAQLADAHVQLAAEVAQAYITLREQQESLAHLTSSEQLEAEMFELTQQRRARGVASQLEVERLMTQLDNTRAQMSPVQASIAESLDQLAMLTGRSPGTLDAELSAPKPLPGVPETVAVGDPGALLKSRPDIRAAERRLASSTAQIGVRKADWFPKLSLLGSLGFAATDPSHLARKENGTWMTLPRLQWNILDFGRVAAGVEGAEGGRDEAEASYESVVLKALRDADVALSRYGHQREHVVVLRRVEASAERASTLQQQRYRAGTASMLEWLDVERTRVVAQQSRISGDADLLKDFVSLQKSLGLGWQSSAAPAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="107" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LBS2</accession>
    <name>Q1LBS2_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Alcohol dehydrogenase, NAD-dependent, Zn-containing</fullName>
            <ecNumber evidence="4">1.1.1.1</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">Rmet_5545</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="5">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="5">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the zinc-containing alcohol dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.1" evidence="4"/>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12404.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519948.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5545"/>
    <dbReference type="EnsemblBacteria" id="ABF12404">
        <property type="protein sequence ID" value="ABF12404"/>
        <property type="gene ID" value="Rmet_5545"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5545"/>
    <dbReference type="eggNOG" id="COG1064">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026673_11_2_4"/>
    <dbReference type="OMA" id="LDCRQCK"/>
    <dbReference type="OrthoDB" id="969875at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004022">
        <property type="term" value="F:alcohol dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013149">
        <property type="entry name" value="ADH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013154">
        <property type="entry name" value="ADH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002328">
        <property type="entry name" value="ADH_Zn_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020843">
        <property type="entry name" value="PKS_ER"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08240">
        <property type="entry name" value="ADH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00107">
        <property type="entry name" value="ADH_zinc_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00829">
        <property type="entry name" value="PKS_ER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="SSF50129"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00059">
        <property type="entry name" value="ADH_ZINC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1 4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-0614">Plasmid</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="PKS_ER" evidence="3">
        <location>
            <begin position="2"/>
            <end position="348"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU361277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ABF12404.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="352" mass="36725" checksum="BC4485C84609EA8D" modified="2006-05-30" version="1">MKSYDIVEWGKPLQEVLRDRPKPVGTEVLVKVQACGICHSDLHIRDGLLDLGNGKHISFESVGVKLPFTMGHEIVGVVAEAGSEASVKAGTPCVVYPWHGCGTCLNCTNGREVDCESGRALGTRKPGGYSDYVVVPHEQYLLDYGNLDPLLAATCACSGLTSFSALKKLPPMLEADTLVLIGAGGLGLAALGLAPQLTSARLVVADIDDEKLARASAAGAHEVVNLRSENAARDLRKAAPNGIRAIVDFVGSAQTTGVALESVARGGTVVVVGLFGGAISLSTALLPMRNVTLRGSYVGSVEEMRALLDLLQEKGKGSPVNLKSLPMSAVNEALEELAQGRVAGRLLAIPGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LBT4</accession>
    <name>Q1LBT4_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Nitroreductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_5533</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12392.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519937.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5533"/>
    <dbReference type="EnsemblBacteria" id="ABF12392">
        <property type="protein sequence ID" value="ABF12392"/>
        <property type="gene ID" value="Rmet_5533"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5533"/>
    <dbReference type="eggNOG" id="COG0778">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_070764_9_1_4"/>
    <dbReference type="OMA" id="WEFFRAP"/>
    <dbReference type="OrthoDB" id="1847197at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.109.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029479">
        <property type="entry name" value="Nitroreductase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000415">
        <property type="entry name" value="Nitroreductase-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00881">
        <property type="entry name" value="Nitroreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55469">
        <property type="entry name" value="SSF55469"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nitroreductase" evidence="1">
        <location>
            <begin position="17"/>
            <end position="204"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF12392.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="230" mass="25375" checksum="589F2BC967F2B005" modified="2006-05-30" version="1">MSIREQLPSVVLEGLLAARHSCRAFLPDEVPQDIVERIVSLAQRTASWCNAQPWQVVITRGAATDRFREVMLAPSESDDEKRPDFEWPREYQGVYQERRRECGLALYDAVGVARGDREASTRQARENFRLFGAPHVAIITSDEALSTYGAVDCGGYVANFMLAATSLGVATIAQAALAARPHRVRECLGLPASRRIVCGISFGYPDTHHPANGFRTSRAELSSAVKWLDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-01-19" version="70" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LBX4</accession>
    <name>Q1LBX4_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">NIPSNAP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_5493</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12352.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519898.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5493"/>
    <dbReference type="EnsemblBacteria" id="ABF12352">
        <property type="protein sequence ID" value="ABF12352"/>
        <property type="gene ID" value="Rmet_5493"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5493"/>
    <dbReference type="eggNOG" id="ENOG5032VT3">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_097061_2_1_4"/>
    <dbReference type="OMA" id="VVHLWGY"/>
    <dbReference type="OrthoDB" id="1925073at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011008">
        <property type="entry name" value="Dimeric_a/b-barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012577">
        <property type="entry name" value="NIPSNAP"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07978">
        <property type="entry name" value="NIPSNAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54909">
        <property type="entry name" value="SSF54909"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NIPSNAP" evidence="1">
        <location>
            <begin position="11"/>
            <end position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF07978"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF12352.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="115" mass="13227" checksum="697EBF5147D57285" modified="2006-05-30" version="1">MTAIARPLVDHRVYTIALRKMPEFMEVFNRLAMPILMETLEHPLGFYTSMVGPQNQFVHLWAYEDLADYERRCRARDCHPRFGEYLAASGHLITAQETRLIRTVDGFVATAAARA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2020-10-07" version="56" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LBZ9</accession>
    <name>Q1LBZ9_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_5468</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12327.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5468"/>
    <dbReference type="EnsemblBacteria" id="ABF12327">
        <property type="protein sequence ID" value="ABF12327"/>
        <property type="gene ID" value="Rmet_5468"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5468"/>
    <dbReference type="eggNOG" id="COG0457">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_150629_0_0_4"/>
    <dbReference type="OrthoDB" id="1837307at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193047" evidence="1">
        <location>
            <begin position="26"/>
            <end position="148"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF12327.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="148" mass="16138" checksum="16FC147C6C660C8B" modified="2006-05-30" version="1">MWKNGRLFAFLVAASLGGLASEASAQSTALLLFGGTSHKTFLGCLNCGQYESSSVCNAYGEFGSPYQADSIWNPYGTYGSKYNNESPWNTYGTSAPAIVDKDGNFYGYLSANRYLAKRTTIKGLVALTEAAEEFDDLEKLSDAFCRRR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2006-07-11" modified="2022-08-03" version="107" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LCS4</accession>
    <name>3HAO_CUPMC</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">3-hydroxyanthranilate 3,4-dioxygenase</fullName>
            <ecNumber evidence="1">1.13.11.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">3-hydroxyanthranilate oxygenase</fullName>
            <shortName evidence="1">3-HAO</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">3-hydroxyanthranilic acid dioxygenase</fullName>
            <shortName evidence="1">HAD</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">nbaC</name>
        <name type="ordered locus">Rmet_5193</name>
    </gene>
    <organism>
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid">
        <name>megaplasmid</name>
    </geneLocation>
    <reference key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2005" name="Biochemistry" volume="44" first="7623" last="7631">
            <title>The mechanism of inactivation of 3-hydroxyanthranilate-3,4-dioxygenase by 4-chloro-3-hydroxyanthranilate.</title>
            <authorList>
                <person name="Colabroy K.L."/>
                <person name="Zhai H."/>
                <person name="Li T."/>
                <person name="Ge Y."/>
                <person name="Zhang Y."/>
                <person name="Liu A."/>
                <person name="Ealick S.E."/>
                <person name="McLafferty F.W."/>
                <person name="Begley T.P."/>
            </authorList>
            <dbReference type="PubMed" id="15909977"/>
            <dbReference type="DOI" id="10.1021/bi0473455"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>ACTIVITY REGULATION</scope>
        <scope>IDENTIFICATION BY MASS SPECTROMETRY</scope>
    </reference>
    <reference key="3">
        <citation type="journal article" date="2005" name="Biochemistry" volume="44" first="7632" last="7643">
            <title>Structural studies on 3-hydroxyanthranilate-3,4-dioxygenase: the catalytic mechanism of a complex oxidation involved in NAD biosynthesis.</title>
            <authorList>
                <person name="Zhang Y."/>
                <person name="Colabroy K.L."/>
                <person name="Begley T.P."/>
                <person name="Ealick S.E."/>
            </authorList>
            <dbReference type="PubMed" id="15909978"/>
            <dbReference type="DOI" id="10.1021/bi047353l"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (1.9 ANGSTROMS) OF NATIVE PROTEIN AND COMPLEXES WITH SUBSTRATES OR INHIBITOR AND IRON</scope>
        <scope>COFACTOR</scope>
        <scope>KINETIC PARAMETERS</scope>
        <scope>MUTAGENESIS OF ARG-47; ARG-99 AND GLU-110</scope>
        <scope>SUBUNIT</scope>
        <scope>REACTION MECHANISM</scope>
    </reference>
    <comment type="function">
        <text evidence="1 2">Catalyzes the oxidative ring opening of 3-hydroxyanthranilate to 2-amino-3-carboxymuconate semialdehyde, which spontaneously cyclizes to quinolinate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>3-hydroxyanthranilate + O2 = (2Z,4Z)-2-amino-3-carboxymuconate 6-semialdehyde</text>
            <dbReference type="Rhea" id="RHEA:17953"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:36559"/>
            <dbReference type="ChEBI" id="CHEBI:77612"/>
            <dbReference type="EC" id="1.13.11.6"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 3">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
        <text evidence="1 3">Binds 2 Fe(2+) ions per subunit.</text>
    </comment>
    <comment type="activity regulation">
        <text evidence="2">Inhibited by 4-chloro-3-hydroxyanthranilate. Mechanism of inactivation involves the oxidation of the catalytic active site Fe(2+) to the catalytically inactive Fe(3+) oxidation state, superoxide production, and formation of two disulfide bonds between Cys-125 and Cys-128, and Cys-162 and Cys-165. Enzyme can be reactivated under reducing conditions.</text>
    </comment>
    <comment type="biophysicochemical properties">
        <kinetics>
            <KM evidence="3">22.4 uM for 3-hydroxyanthranilate</KM>
        </kinetics>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; NAD(+) biosynthesis; quinolinate from L-kynurenine: step 3/3.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1 3">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the 3-HAO family.</text>
    </comment>
    <dbReference type="EC" id="1.13.11.6" evidence="1"/>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF12052.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519599.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="PDB" id="1YFU">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="1.90 A"/>
        <property type="chains" value="A=1-174"/>
    </dbReference>
    <dbReference type="PDB" id="1YFW">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.00 A"/>
        <property type="chains" value="A=1-174"/>
    </dbReference>
    <dbReference type="PDB" id="1YFX">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.00 A"/>
        <property type="chains" value="A=1-174"/>
    </dbReference>
    <dbReference type="PDB" id="1YFY">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="3.20 A"/>
        <property type="chains" value="A=1-174"/>
    </dbReference>
    <dbReference type="PDB" id="4HSJ">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="1.88 A"/>
        <property type="chains" value="A=1-174"/>
    </dbReference>
    <dbReference type="PDB" id="4HSL">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.00 A"/>
        <property type="chains" value="A=1-174"/>
    </dbReference>
    <dbReference type="PDB" id="4HVO">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="1.75 A"/>
        <property type="chains" value="A=1-174"/>
    </dbReference>
    <dbReference type="PDB" id="4HVQ">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.81 A"/>
        <property type="chains" value="A=1-154"/>
    </dbReference>
    <dbReference type="PDB" id="4HVR">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.70 A"/>
        <property type="chains" value="A=1-174"/>
    </dbReference>
    <dbReference type="PDB" id="4I3P">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="1.96 A"/>
        <property type="chains" value="A=1-174"/>
    </dbReference>
    <dbReference type="PDB" id="4L2N">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="1.74 A"/>
        <property type="chains" value="A=1-174"/>
    </dbReference>
    <dbReference type="PDB" id="4R52">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="1.53 A"/>
        <property type="chains" value="A=1-174"/>
    </dbReference>
    <dbReference type="PDB" id="4WZC">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="1.84 A"/>
        <property type="chains" value="A=1-174"/>
    </dbReference>
    <dbReference type="PDB" id="5V26">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="1.79 A"/>
        <property type="chains" value="A/B=1-174"/>
    </dbReference>
    <dbReference type="PDB" id="5V27">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.35 A"/>
        <property type="chains" value="A=1-174"/>
    </dbReference>
    <dbReference type="PDB" id="5V28">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.72 A"/>
        <property type="chains" value="A=1-174"/>
    </dbReference>
    <dbReference type="PDB" id="6BVP">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="1.90 A"/>
        <property type="chains" value="A=1-174"/>
    </dbReference>
    <dbReference type="PDB" id="6BVQ">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.08 A"/>
        <property type="chains" value="A=1-174"/>
    </dbReference>
    <dbReference type="PDB" id="6BVR">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="1.90 A"/>
        <property type="chains" value="A=1-174"/>
    </dbReference>
    <dbReference type="PDB" id="6BVS">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.32 A"/>
        <property type="chains" value="A=1-174"/>
    </dbReference>
    <dbReference type="PDB" id="6CD3">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.61 A"/>
        <property type="chains" value="A=1-174"/>
    </dbReference>
    <dbReference type="PDB" id="6D60">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.22 A"/>
        <property type="chains" value="A=1-174"/>
    </dbReference>
    <dbReference type="PDB" id="6D61">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="1.74 A"/>
        <property type="chains" value="A=1-174"/>
    </dbReference>
    <dbReference type="PDB" id="6D62">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="1.77 A"/>
        <property type="chains" value="A=1-174"/>
    </dbReference>
    <dbReference type="PDB" id="6VI5">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="1.60 A"/>
        <property type="chains" value="A=1-174"/>
    </dbReference>
    <dbReference type="PDB" id="6VI6">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="1.90 A"/>
        <property type="chains" value="A=1-174"/>
    </dbReference>
    <dbReference type="PDB" id="6VI7">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.62 A"/>
        <property type="chains" value="A=1-174"/>
    </dbReference>
    <dbReference type="PDB" id="6VI8">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="1.95 A"/>
        <property type="chains" value="A=1-174"/>
    </dbReference>
    <dbReference type="PDB" id="6VI9">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.31 A"/>
        <property type="chains" value="A=1-174"/>
    </dbReference>
    <dbReference type="PDB" id="6VIA">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="1.59 A"/>
        <property type="chains" value="A=1-174"/>
    </dbReference>
    <dbReference type="PDB" id="6VIB">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="1.84 A"/>
        <property type="chains" value="A=1-174"/>
    </dbReference>
    <dbReference type="PDB" id="6X11">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.10 A"/>
        <property type="chains" value="A=1-174"/>
    </dbReference>
    <dbReference type="PDBsum" id="1YFU"/>
    <dbReference type="PDBsum" id="1YFW"/>
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</entry>
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                <person name="Van Houdt R."/>
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                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    </evidence>
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    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
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    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="157"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="158"/>
            <end position="182"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF11983.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="182" mass="19353" checksum="7558488806180D46" modified="2006-05-30" version="1">MAMLRSAATDPMLDATRAFLENSYKFAALAAETRRNLIETHLKSLEHRLTAMHSAQSRMAGVADWASLQAAQAQTLTDQAEAQAALARAYGKVLVEATSAWLEQCRECSSDWVKRVEATNETARGNGNGNGAAVLDPLSASLAEFYERLGKMTPFGVVPPSESSTTATSATRSRAAKGGATS</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="58" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LD34</accession>
    <name>Q1LD34_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_5083</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11942.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519489.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5083"/>
    <dbReference type="EnsemblBacteria" id="ABF11942">
        <property type="protein sequence ID" value="ABF11942"/>
        <property type="gene ID" value="Rmet_5083"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5083"/>
    <dbReference type="eggNOG" id="ENOG5031XRK">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_082347_0_0_4"/>
    <dbReference type="OMA" id="PVRFHFI"/>
    <dbReference type="OrthoDB" id="1625042at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021549">
        <property type="entry name" value="DUF2894"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11445">
        <property type="entry name" value="DUF2894"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF11942.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="232" mass="25067" checksum="E1989A3B8D61B2E9" modified="2006-05-30" version="1">MHNDGPHDPLTLLDGWRDSGADRADPVRFRFIETMAQRASGYSGEAGRLLDARVARLVAEYGAMLARDAGASDEVASPEAQSQPHSALAELAALRERLGQHTQPATGAITSHTHANANAQAQAHTPNALIPQRAAPVEPALADYFRDTWARVSVDRQMRQSQAGVPENAGPLNTNHLVHRSLSLMREVSPGYLEQFLSYVDALSWLEGMHPISLVPERKARAKSGKTGSGIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LDA5</accession>
    <name>Q1LDA5_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Putative flavoprotein oxidoreductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_5009</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11871.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519420.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5009"/>
    <dbReference type="EnsemblBacteria" id="ABF11871">
        <property type="protein sequence ID" value="ABF11871"/>
        <property type="gene ID" value="Rmet_5009"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5009"/>
    <dbReference type="eggNOG" id="COG2081">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025174_1_0_4"/>
    <dbReference type="OMA" id="RHRWLGW"/>
    <dbReference type="OrthoDB" id="1513550at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.260">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004792">
        <property type="entry name" value="BaiN-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022460">
        <property type="entry name" value="Flavoprotein_PP4765"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023166">
        <property type="entry name" value="HI0933-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42887">
        <property type="entry name" value="PTHR42887"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="SSF51905"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03862">
        <property type="entry name" value="flavo_PP4765"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00275">
        <property type="entry name" value="TIGR00275"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF11871.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="421" mass="44665" checksum="14DE5BCA272E696D" modified="2006-05-30" version="1">MPAVPAQEPIRHDGAIVIGGGPAGLMAAEMLAGQGLPVDVYDAMPSVGRKFLMAGKGGMNITHSEAAEAFLRRYGARADRIGPLLDSFDADKLRAWVHGLGIDTFVGSSGRVFPTDMKAAPLLRAWLHRLRESGVRIHVRHRWIGFGAPIDGWHVLRFATRDGEIEVAARAVVLALGGGSWARLGSDGAWLPMLAARGIDVAPLRPANCGFDLAWNEVFRAQHAGEPVKSVAIGITTVDGTALQRQGEFVISETGIEGSLVYALSAPIRDLIETHGFAVITVDLAPGRSPEDVAAEVSRPRGSRSVSSHLQSRLGITGVKARMLREVLTKEAMHDPVALAAAIKALPLRLVRARPIDEVISTAGGVRFEAMDDRLMLTALPGVFCAGEMLDWEAPTGGYLLTACFASGRVAGSGAVAYLRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LDA9</accession>
    <name>Q1LDA9_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative isochorismatase hydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_5005</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11867.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519417.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_5005"/>
    <dbReference type="EnsemblBacteria" id="ABF11867">
        <property type="protein sequence ID" value="ABF11867"/>
        <property type="gene ID" value="Rmet_5005"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_5005"/>
    <dbReference type="eggNOG" id="COG1335">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_068979_3_0_4"/>
    <dbReference type="OMA" id="YTVKDCC"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.850">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000868">
        <property type="entry name" value="Isochorismatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036380">
        <property type="entry name" value="Isochorismatase-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00857">
        <property type="entry name" value="Isochorismatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52499">
        <property type="entry name" value="SSF52499"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Isochorismatase" evidence="1">
        <location>
            <begin position="11"/>
            <end position="180"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF11867.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="189" mass="20483" checksum="84CD2E4F2ECFD341" modified="2006-05-30" version="1">MQIPVIEPARTALLVMHYQTDILELFPSVAPVLLSNTRRLCDAARAVGVGVYFAKIHFSAGYPEVSPLNRNGQVIKQLGLFVNDQISPELGQRADEPLILAHRASVFFGTDLEVRLSARGIDTLIMVGIASTGVVLSSVAHASDADFRLLTVKDCCYDPDPVVHDHLFATAFESRTTVLSLIDALALLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-08-03" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LDC0</accession>
    <name>Q1LDC0_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Porin, Gram-negative type</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ordered locus">Rmet_4994</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="8">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="8">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11856.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519405.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4994"/>
    <dbReference type="EnsemblBacteria" id="ABF11856">
        <property type="protein sequence ID" value="ABF11856"/>
        <property type="gene ID" value="Rmet_4994"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4994"/>
    <dbReference type="eggNOG" id="COG3203">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038238_2_0_4"/>
    <dbReference type="OrthoDB" id="1064531at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046930">
        <property type="term" value="C:pore complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015288">
        <property type="term" value="F:porin activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006811">
        <property type="term" value="P:ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00342">
        <property type="entry name" value="gram_neg_porins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.160.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033900">
        <property type="entry name" value="Gram_neg_porin_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023614">
        <property type="entry name" value="Porin_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002299">
        <property type="entry name" value="Porin_Neis"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13609">
        <property type="entry name" value="Porin_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00184">
        <property type="entry name" value="NEISSPPORIN"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0406">Ion transport</keyword>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0614">Plasmid</keyword>
    <keyword evidence="4" id="KW-0626">Porin</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1134">Transmembrane beta strand</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5004193084" evidence="5">
        <location>
            <begin position="21"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="domain" description="Porin_4" evidence="6">
        <location>
            <begin position="7"/>
            <end position="345"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF13609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABF11856.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="381" mass="40570" checksum="529E2E19E03A723F" modified="2006-05-30" version="1">MRKQAFALAALAAASVTAHAQSSVTLYGVIDGSIEYVNRVASSAATPNQGGSRFGMPSVGGLSASRWGLRGVEDLGGGNQALFVLESGFQWDTGALQSAPLFNRQSYLGIQNATVGKFSFGRQYTSFFDGMANFAPLRFAATYEPGIWWMGLNYREDNMVKYTGQFGPFQAVAHFSFGTGVAVQNATILLANGGAGEVPGHFSDNTGYGGSLMYLSNGLGFSLAADEWRPAAVTGQPGKMTKLGAAALYNTGPLKFTAGYRYNKGEFSNGGTLLRDDYWWAGVNYQATPALGLSLAYYFANVKQASAAANTPTSNPPNMHQVSFIADYNFSKRTDVYLSAGWAHNGGLSFDGAATAYAFKYPTMTGQQNMIGVTVGIRQIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LDS8</accession>
    <name>Q1LDS8_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Putative diguanylate phosphodiesterase (EAL domain)</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ordered locus">Rmet_4836</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="3">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="3">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11698.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519262.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4836"/>
    <dbReference type="EnsemblBacteria" id="ABF11698">
        <property type="protein sequence ID" value="ABF11698"/>
        <property type="gene ID" value="Rmet_4836"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4836"/>
    <dbReference type="eggNOG" id="COG2200">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_70_50_4"/>
    <dbReference type="OMA" id="INVFPST"/>
    <dbReference type="OrthoDB" id="1635706at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="CDD" id="cd01948">
        <property type="entry name" value="EAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.450">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001633">
        <property type="entry name" value="EAL_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035919">
        <property type="entry name" value="EAL_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00563">
        <property type="entry name" value="EAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00052">
        <property type="entry name" value="EAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF141868">
        <property type="entry name" value="SSF141868"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50883">
        <property type="entry name" value="EAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0614">Plasmid</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="EAL" evidence="1">
        <location>
            <begin position="1"/>
            <end position="248"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ABF11698.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="254" mass="27679" checksum="1B97F15307992A04" modified="2006-05-30" version="1">MTPTIADCLEQRYLRPVFQPIGSLATGDILGYEALIRGPVGTPLENPVALFAEAARAGALVRMERLAARTAIAAFMQARLPGKLFLNYSADSLRDIEDTREDVRNFLQRVDMPSDRIVLELTEQAPLGPLESLTKAVFSIRARGAQFALDDYGTGHANLGQWIAMKPDYVKIARVVIDGIATSTFQLEAVRALCKLAKLSGTKLVAEGIERVDDLLVCRELGIDYAQGYLLARPAEVPALTLSEEALRALRAAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LDU5</accession>
    <name>Q1LDU5_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Nitrite reductase, NAD(P)H-binding, small subunit</fullName>
            <ecNumber evidence="7">1.7.1.4</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">nirD</name>
        <name evidence="7" type="ordered locus">Rmet_4819</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="8">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="8">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EC" id="1.7.1.4" evidence="7"/>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11681.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519244.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4819"/>
    <dbReference type="EnsemblBacteria" id="ABF11681">
        <property type="protein sequence ID" value="ABF11681"/>
        <property type="gene ID" value="Rmet_4819"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4819"/>
    <dbReference type="eggNOG" id="COG2146">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_055690_3_0_4"/>
    <dbReference type="OMA" id="AAPLYKQ"/>
    <dbReference type="OrthoDB" id="1681886at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008942">
        <property type="term" value="F:nitrite reductase [NAD(P)H] activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019439">
        <property type="term" value="P:aromatic compound catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042128">
        <property type="term" value="P:nitrate assimilation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03529">
        <property type="entry name" value="Rieske_NirD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.102.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017881">
        <property type="entry name" value="NirD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012748">
        <property type="entry name" value="Rieske-like_NirD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017941">
        <property type="entry name" value="Rieske_2Fe-2S"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036922">
        <property type="entry name" value="Rieske_2Fe-2S_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40562">
        <property type="entry name" value="PTHR40562"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13806">
        <property type="entry name" value="Rieske_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50022">
        <property type="entry name" value="SSF50022"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02378">
        <property type="entry name" value="nirD_assim_sml"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51296">
        <property type="entry name" value="RIESKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0058">Aromatic hydrocarbons catabolism</keyword>
    <keyword evidence="3" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0534">Nitrate assimilation</keyword>
    <keyword evidence="7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-0614">Plasmid</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rieske" evidence="6">
        <location>
            <begin position="11"/>
            <end position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022797"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ABF11681.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="133" mass="14473" checksum="DB89A6A7BD86DF1D" modified="2006-05-30" version="1">MSQAHHPETWTAICTLRDIVPNTGVCALVDRKQVAVFRIGRGEEVYALANFDPNAQANVLSRGLVGNLGPRTVVASPIYKHHFDLRTGECLEAPEHSVDAYTARVYDGKVWVATAATAREVEAEAEAEEELAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2021-06-02" version="57" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LE43</accession>
    <name>Q1LE43_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ordered locus">Rmet_4721</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid" evidence="2">
        <name>megaplasmid CH34</name>
    </geneLocation>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
            <authorList>
                <person name="Janssen P.J."/>
                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
                <person name="Leys N."/>
                <person name="Vallaeys T."/>
                <person name="Lapidus A."/>
                <person name="Monchy S."/>
                <person name="Medigue C."/>
                <person name="Taghavi S."/>
                <person name="McCorkle S."/>
                <person name="Dunn J."/>
                <person name="van der Lelie D."/>
                <person name="Mergeay M."/>
            </authorList>
            <dbReference type="PubMed" id="20463976"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0010433"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">ATCC 43123 / DSM 2839 / NBRC 102507 / CH34</strain>
            <plasmid evidence="2">Plasmid megaplasmid CH34</plasmid>
        </source>
    </reference>
    <dbReference type="EMBL" id="CP000353">
        <property type="protein sequence ID" value="ABF11583.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_011519155.1">
        <property type="nucleotide sequence ID" value="NC_007974.2"/>
    </dbReference>
    <dbReference type="STRING" id="266264.Rmet_4721"/>
    <dbReference type="EnsemblBacteria" id="ABF11583">
        <property type="protein sequence ID" value="ABF11583"/>
        <property type="gene ID" value="Rmet_4721"/>
    </dbReference>
    <dbReference type="KEGG" id="rme:Rmet_4721"/>
    <dbReference type="eggNOG" id="ENOG5033AMK">
        <property type="taxonomic scope" value="Bacteria"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_134953_1_0_4"/>
    <dbReference type="OMA" id="EQFAWLH"/>
    <dbReference type="OrthoDB" id="1658250at2"/>
    <dbReference type="Proteomes" id="UP000002429">
        <property type="component" value="Plasmid megaplasmid CH34"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0614">Plasmid</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ABF11583.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000002429"/>
        </source>
    </evidence>
    <sequence length="141" mass="16126" checksum="D475E56C30FD8391" modified="2006-05-30" version="1">MDMPSEQFLAIQETRRLGLRALRAALLQVHKEVIGYDRGQYERLHGPIPAGQFVQLVTEESFFRWLDPLSRLIIEIDEELDGEEHHDETCRAVAAAAQKLFSERGDADFRKRYQEALQDEAAVTVAHGRLMSVIGQLRQLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2006-05-30" modified="2022-05-25" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>Q1LE96</accession>
    <name>Q1LE96_CUPMC</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Hydrogenase 4 subunit F</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">hyfF</name>
        <name evidence="4" type="ordered locus">Rmet_4668</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)</name>
        <name type="synonym">Ralstonia metallidurans</name>
        <dbReference type="NCBI Taxonomy" id="266264"/>
        <lineage>
            <taxon>Bacteria</taxon>
            <taxon>Proteobacteria</taxon>
            <taxon>Betaproteobacteria</taxon>
            <taxon>Burkholderiales</taxon>
            <taxon>Burkholderiaceae</taxon>
            <taxon>Cupriavidus</taxon>
        </lineage>
    </organism>
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                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
                <person name="Michaux A."/>
                <person name="Benotmane M.A."/>
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                <person name="Vallaeys T."/>
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</entry>
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        <citation type="journal article" date="2010" name="PLoS ONE" volume="5" first="E10433" last="E10433">
            <title>The complete genome sequence of Cupriavidus metallidurans strain CH34, a master survivalist in harsh and anthropogenic environments.</title>
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                <person name="Van Houdt R."/>
                <person name="Moors H."/>
                <person name="Monsieurs P."/>
                <person name="Morin N."/>
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